gebhart/patrepeval huggingface dataset:
Retroviral Gene Transfer into the Intestinal Epithelium (1995)

The epithelial cells of the gastrointestinal tract may be attractive targets for somatic gene therapy. In these studies, we have used rats and mice to explore the feasibility of gene transfer into the small intestinal epithelium using retroviral vectors. The first series of experiments was conducted in mature Sprague-Dawley rats using an ecotropic retroviral vector that has bacterial beta-galactosidase (beta-Gal) as the reporter gene. The vector was introduced into the lumen of ligated segments of terminal ileum. After a 4-hr exposure period, the ligatures were removed. Sham-operated animals were subjected to the same ligation procedure but received only tissue culture medium in the ligated segment. All animals were sacrificed 6 days later, and tissue from both the experimental segment and an upstream control segment was assessed for cytoplasmic beta-Gal activity using X-Gal histochemistry. Expression of the reporter gene was observed in the crypt epithelium of tissue exposed to the vector. In the villus epithelium, high background staining precluded accurate assessment of reporter gene expression. To obviate the latter problem, we sought an alternative reporter gene for which there would be no background staining in control animals. We repeated the experiments with beta-glucuronidase as the reporter gene in MPS VII mutant mice, which are devoid of this enzyme. In these studies, ileal segments exposed to the vector demonstrated expression of the reporter gene in both the crypt and villus epithelium 4 days after exposure. These results indicate that genes can be transferred into the intestinal epithelium using retroviral vectors introduced luminally.(ABSTRACT TRUNCATED AT 250 WORDS)
SIFT-BASED MEASUREMENTS FOR VEHICLE MODEL RECOGNITION (2009)

Model Recognition (VMMR) method was utilized to tackle the problem of vehicle security. Distinctive parts of the vehicle frontal view such as the headlights, grill and logo area were segmented. A series of experiments were conducted in a variety of outdoor conditions, where a query image that was rotated, scaled, shifted or set in different lighting conditions, matched against a database of model images. In this work, is shown that image processing functions based on Scale Invariant Feature Transform (SIFT) measurements can be used to obtain high performance object features recognition, creating a keypoint fingerprint (pattern) for each image class. In the majority of the cases, SIFT method performs very well, in terms of efficiency and robustness.
Native and recombinant human hepatocyte growth factors are highly potent promoters of DNA synthesis in both human and rat hepatocytes. (1991)

Human hepatocyte growth factor (hHGF) has recently been expressed as a recombinant polypeptide from Chinese hampster ovary cell transfectants. Using a primary rat hepatocyte bioassay, we have tested the biological activity of recombinant hHGF and compared it with native hHGF. Dose-response curves were almost identical, with half-maximal stimulation of DNA synthesis at 1-2 ng/ml (equivalent to approximately 10 pM). S-phase labeling index was similarly enhanced and numerous mitotic cells were observed. Recombinant and native hHGF also stimulated DNA synthesis and S-phase labeling index in primary adult human hepatocytes. Human cells were more responsive than rat hepatocytes, with recombinant hHGF slightly more potent than native hHGF (half-maximal stimulation 0.3 and 0.6 ng/ml, respectively). Since HGF levels rise in patients with fulminant hepatic failure and in animals after partial hepatectomy or administration of hepatotoxins, situations where liver regeneration occurs, HGF is suggested to play a key role in regulation of hepatic growth. The high potency of the factor on human hepatocytes reinforces its candidacy as a critical mitogen in human liver growth. The availability of a recombinant hHGF opens the way for in vivo experimental studies and to the possibility of using hHGF as a clinical therapeutic agent, either alone or in combination with other factors.
Altered Glycosylation of 63- and 68-Kilodalton Microvillar Proteins in <i>Heliothis virescens</i> Correlates with Reduced Cry1 Toxin Binding, Decreased Pore Formation, and Increased Resistance to <i>Bacillus thuringiensis</i> Cry1 Toxins (2002)

The binding and pore formation abilities of Cry1A and Cry1Fa Bacillus thuringiensis toxins were analyzed by using brush border membrane vesicles (BBMV) prepared from sensitive (YDK) and resistant (YHD2) strains of Heliothis virescens. 125I-labeled Cry1Aa, Cry1Ab, and Cry1Ac toxins did not bind to BBMV from the resistant YHD2 strain, while specific binding to sensitive YDK vesicles was observed. Binding assays revealed a reduction in Cry1Fa binding to BBMV from resistant larvae compared to Cry1Fa binding to BBMV from sensitive larvae. In agreement with this reduction in binding, neither Cry1A nor Cry1Fa toxin altered the permeability of membrane vesicles from resistant larvae, as measured by a light-scattering assay. Ligand blotting experiments performed with BBMV and 125I-Cry1Ac did not differentiate sensitive larvae from resistant larvae. Iodination of BBMV surface proteins suggested that putative toxin-binding proteins were exposed on the surface of the BBMV from resistant insects. BBMV protein blots probed with the N-acetylgalactosamine-specific lectin soybean agglutinin (SBA) revealed altered glycosylation of 63- and 68-kDa glycoproteins but not altered glycosylation of known Cry1 toxin-binding proteins in YHD2 BBMV. The F1 progeny of crosses between sensitive and resistant insects were similar to the sensitive strain when they were tested by toxin-binding assays, light-scattering assays, and lectin blotting with SBA. These results are evidence that a dramatic reduction in toxin binding is responsible for the increased resistance and cross-resistance to Cry1 toxins observed in the YHD2 strain of H. virescens and that this trait correlates with altered glycosylation of specific brush border membrane glycoproteins.
Variable-Rate Transmission for Rayleigh Fading Channels (1972)

A modulation system is proposed that continuously adjusts its data rate in response to signal strength variations in a fading channel. The optimum variation of data rate with channel conditions is determined, and includes the effects of feedback channel time delay, the interval between rate changes, and restriction of the number of allowable rates. Application of these results to a full duplex situation allows determination of the optimum fraction of the data stream to be devoted to service information. Comparison of this scheme with diversity transmission on the basis of error probability and bandwidth utilization reveals a reduction on the order of 14 dB in transmitter power for a typical duplex link.
Analogy‐driven 3D style transfer (2014)

Abstract Style transfer aims to apply the style of an exemplar model to a target one, while retaining the target's structure. The main challenge in this process is to algorithmically distinguish style from structure, a high‐level, potentially ill‐posed cognitive task. Inspired by cognitive science research we recast style transfer in terms of shape analogies . In IQ testing, shape analogy queries present the subject with three shapes: source, target and exemplar , and ask them to select an output such that the transformation, or analogy , from the exemplar to the output is similar to that from the source to the target. The logical process involved in identifying the source‐to‐target analogies implicitly detects the structural differences between the source and target and can be used effectively to facilitate style transfer. Since the exemplar has a similar structure to the source, applying the analogy to the exemplar will provide the output we seek. The main technical challenge we address is to compute the source to target analogies, consistent with human logic. We observe that the typical analogies we look for consist of a small set of simple transformations, which when applied to the exemplar generate a continuous, seamless output model. To assemble a shape analogy, we compute an optimal set of source‐to‐target transformations, such that the assembled analogy best fits these criteria. The assembled analogy is then applied to the exemplar shape to produce the desired output model. We use the proposed framework to seamlessly transfer a variety of style properties between 2D and 3D objects and demonstrate significant improvements over the state of the art in style transfer. We further show that our framework can be used to successfully complete partial scans with the help of a user provided structural template, coherently propagating scan style across the completed surfaces.
Injection-Locked Push-Push Oscillator at 72 GHz Band Using Cross-Coupled HEMTs (2003)

An injection-locked push-push oscillator at V-band is developed for high purity millimeter-wave signal source. Cross-coupled HEMTs are applied for the negative resistance generation across wide frequency range and virtually grounded short-ended lines are used for the resonant circuit instead of lumped inductors. Thanks to the differential operation of cross-coupled HEMTs, the push-push oscillator was easily implemented by combing the 2 nd harmonics of oscillation signal at the drain terminals. Injection signal at the fundamental oscillation frequency is applied at the gate of current source FET of cross-coupled FETs for stabilizing the oscillation, which performs the amplification and harmonic generation. The designed oscillator was fabricated using 0.15μm GaAs pHEMT process. The measurement showed the free-running oscillation around 35.8 GHz and the maximum lock range of 1.48 GHz (2.1%) around 71.6 GHz with the average output power of - 6 dBm.
10-Gb/s asynchronous digital optical regenerator (1999)

We describe the first experimental demonstration of an asynchronous digital optical regenerator. Error-free regeneration of 10-Gb/s optical packets up to 40000 bits long has been demonstrated without clock recovery. It is demonstrated that the optical nodes in this network may operate with independent clocks, within certain practical limits, offering the possibility of arbitrary mesh optical time-division multiplexing networks.
Hair-crag (1870)

Journal Article Hair-crag Get access W. E. W. E. Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume s4-VI, Issue 147, 22 October 1870, Pages 355–356, https://doi.org/10.1093/nq/s4-VI.147.355-f Published: 22 October 1870
SAW/BAW band reject filters embedded in impedance converter (2016)

This paper discusses SAW-BAW-based band reject filters. They are composed of the impedance converters, where capacitive elements are replaced with SAW/BAW resonators. First, basic properties of the unit cell are studied. It is shown how basic properties of a unit cell change with the design. It is also shown that when two notches caused by the resonators are placed in proximity, two synergy effects occur: (i) an extra matching point appears on one side of the transition band. This make the insertion loss at the point smaller and the transition band steeper, and (ii) the dip level becomes deeper, and the total rejection level becomes better. Then, the wide rejection band filter is designed by cascading multi-stages, and effectiveness of the device configurations is demonstrated.
Development of Artificial Salivas (1993)

Salivary research is at a critical crossroads regarding the clinical application of basic knowledge. Studies by numerous salivary researchers over the last 5 years using advanced technologies (e.g., protein chemistry, molecular biology, and biophysics) have demonstrated that the structural requirements for salivary function are quite complex. Nevertheless, several patterns or principles have evolved. First, the majority if not all salivary molecules are multifunctional. Second, the conformation of a molecule is an important factor in biological activity. Third, many molecules have overlapping functions (e.g., mucins and amylase interact with viridans streptococci; statherin and proline-rich proteins are involved in mineralization). Thus, saliva has "built-in" compensatory or redundant properties. Nevertheless, it must be determined which molecule is more potent or effective with respect to a particular function. Fourth, salivary molecules may be "amphifunctional". In other words, the different functions of a single molecule may be protective or potentially harmful depending on the intraoral site of action. Examples of amphifunctional molecules are amylase and statherin. Fifth, functional relationships may exist between different salivary components. The principles mentioned above can provide experimental strategies for the design and synthesis of a first generation of salivary substitutes that can be topically applied to oral surfaces. These molecules should be used to combat microbial mediated diseases and occlusal disharmony in subjects with normal salivary flow as well as those with xerostomia. In general, these substitutes should be long-lasting, biocompatible, biodegradable, and provide specific protective qualities that can be targeted to selected intraoral sites.(ABSTRACT TRUNCATED AT 250 WORDS)
Sn-Beta zeolites with borate salts catalyse the epimerization of carbohydrates via an intramolecular carbon shift (2012)

Carbohydrate epimerization is an essential technology for the widespread production of rare sugars. In contrast to other enzymes, most epimerases are only active on sugars substituted with phosphate or nucleotide groups, thus drastically restricting their use. Here we show that Sn-Beta zeolite in the presence of sodium tetraborate catalyses the selective epimerization of aldoses in aqueous media. Specifically, a 5 wt% aldose (for example, glucose, xylose or arabinose) solution with a 4:1 aldose:sodium tetraborate molar ratio reacted with catalytic amounts of Sn-Beta yields near-equilibrium epimerization product distributions. The reaction proceeds by way of a 1,2 carbon shift wherein the bond between C-2 and C-3 is cleaved and a new bond between C-1 and C-3 is formed, with C-1 moving to the C-2 position with an inverted configuration. This work provides a general method of performing carbohydrate epimerizations that surmounts the main disadvantages of current enzymatic and inorganic processes.
Insulin-secreting cell lines: classification, characteristics and potential applications. (1996)

The use of primary beta-cells in biochemical and molecular research is limited by the availability of pancreatic endocrine tissue. Numerous investigators have attempted to establish an insulin-secreting cell line that retains normal regulation of insulin secretion. Different approaches have been used, including induction of pancreatic tumors by irradiation or viral infection, immortalization of beta-cells in vitro, and development of transgenic mice with targeted expression of a recombinant oncogene in the beta-cell. Few of these attempts have proven successful, because cell differentiation and proliferation capacities are mutually exclusive. The most widely used insulin-secreting cell lines are RIN, HIT, beta TC, MIN6 and INS-1 cells. These cells contain mainly insulin and small amounts of glucagon and somatostatin. RIN cells, except for the subclone RIN-38, are not glucose-responsive. HIT cells and beta TC cells secrete insulin in response to glucose, but their dose-response curve is markedly shifted to the left MIN6, INS-1 and a newly available subclone of beta TC cells (beta TC-6 F7) are reported to retain normal regulation of glucose-induced insulin secretion. Although the behaviour of none of these cell lines perfectly mimics primary beta-cell physiology, they are extremely valuable tools for the study of molecular events underlying beta-cell function and dysfunction. In addition, insulin-secreting cell lines represent a potential source of transplantable tissue to overcome the limited availability of primary islets for this procedure.
Automatic Facial Feature Extraction for Face Recognition (2007)

The subject of this chapter is the presentation of a novel method for the automatic and precise localization of facial features in 2D still images. The method follows the top-down paradigm and consists of subsequent steps to decompose the initial problem in increasingly easier tasks: assuming a rough localization of the face in the image, first comes the application of an eye detector with the aim of discriminating between real face regions and possible false positives. The accuracy of the detection is nearly optimal. Successively, an eye locator is applied on a small neighborhood of the detector output to improve the localization precision. Finally, the eye center positions are used to derive 27 facial fiducial points, either extracted directly from the image or inferred on the basis of simple geometrical considerations. The eye localization module has been extensively tested on five publicly available databases with different characteristics to remark its generality. In the overall, the results are comparable to or better than those obtained by the state-of-the-art methods. The performance evaluation is carried out according to two objective performance measures in order to favor the comparison with other techniques. Concerning the fiducial point localization, results on the XM2VTS show high precision. In the last years many research works have pointed out the importance of facial feature localization as the fundamental step for the initialization of other methods, mostly face recognition techniques. In general, not all types of error affect the subsequent processing in the same way: for instance scale errors usually affect a FR technique more than translational
Making better maize plants for sustainable grain production in a changing climate (2015)

Achieving grain supply security with limited arable land is a major challenge in the twenty-first century, owing to the changing climate and increasing global population. Maize plays an increasingly vital role in global grain production. As a C4 plant, maize has a high yield potential. Maize is predicted to become the number one cereal in the world by 2020. However, maize production has plateaued in many countries, and hybrid and production technologies have been fully exploited. Thus, there is an urgent need to shape maize traits and architectures for increased stress tolerance and higher yield in a changing climate. Recent achievements in genomics, proteomics, and metabolomics have provided an unprecedented opportunity to make better maize. In this paper, we discuss the current challenges and potential of maize production, particularly in China. We also highlight the need for enhancing maize tolerance to drought and heat waves, summarize the elite shoot and root traits and phenotypes, and propose an ideotype for sustainable maize production in a changing climate. This will facilitate targeted maize improvement through a conventional breeding program combined with molecular techniques.
<i>Drosophila</i> Nipped-B Protein Supports Sister Chromatid Cohesion and Opposes the Stromalin/Scc3 Cohesion Factor To Facilitate Long-Range Activation of the <i>cut</i> Gene (2004)

The Drosophila melanogaster Nipped-B protein facilitates transcriptional activation of the cut and Ultrabithorax genes by remote enhancers. Sequence homologues of Nipped-B, Scc2 of Saccharomyces cerevisiae, and Mis4 of Schizosaccharomyces pombe are required for sister chromatid cohesion during mitosis. The evolutionarily conserved Cohesin protein complex mediates sister chromatid cohesion, and Scc2 and Mis4 are needed for Cohesin to associate with chromosomes. Here, we show that Nipped-B is also required for sister chromatid cohesion but that, opposite to the effect of Nipped-B, the stromalin/Scc3 component of Cohesin inhibits long-range activation of cut. To explain these findings, we propose a model based on the chromatin domain boundary activities of Cohesin in which Nipped-B facilitates cut activation by alleviating Cohesin-mediated blocking of enhancer-promoter communication.
Ceramic Oxide Coatings for the Corrosion Protection of Silicon Carbide (1991)

Silicon carbide is currently used as a structural material for heat exchanger tubes and related applications because of its excellent thermal properties and oxidation resistance. Silicon carbide suffers corrosion degradation, however, in the aggressive furnace environments of industrial processes for aluminum remelting, advanced glass melting, and waste incineration. Adherent ceramic oxide coatings developed at Solar Turbines Incorporated with the support of the Gas Research Institute, have shown to afford corrosion protection to silicon carbide in a simulated aluminum remelt furnace environment as well as in laboratory type corrosion testing. The coatings are also protective to silicon carbide-based ceramic matrix composites.
Spleens of myelofibrosis patients contain malignant hematopoietic stem cells (2012)

Cancer stem cell behavior is thought to be largely determined by intrinsic properties and by regulatory signals provided by the microenvironment. Myelofibrosis (MF) is characterized by hematopoiesis occurring not only in the marrow but also in extramedullary sites such as the spleen. In order to study the effects of these different microenvironments on primitive malignant hematopoietic cells, we phenotypically and functionally characterized splenic and peripheral blood (PB) MF CD34+ cells from patients with MF. MF spleens contained greater numbers of malignant primitive HPCs than PB. Transplantation of PB MF CD34+ cells into immunodeficient (NOD/SCID/IL2Rγ(null)) mice resulted in a limited degree of donor cell chimerism and a differentiation program skewed toward myeloid lineages. By contrast, transplanted splenic MF CD34+ cells achieved a higher level of chimerism and generated both myeloid and lymphoid cells that contained molecular or cytogenetic abnormalities indicating their malignant nature. Only splenic MF CD34+ cells were able to sustain hematopoiesis for prolonged periods (9 months) and were able to engraft secondary recipients. These data document the existence of MF stem cells (MF-SCs) that reside in the spleens of MF patients and demonstrate that these MF-SCs retain a differentiation program identical to that of normal hematopoietic stem cells.
Complement (C5)-derived chemotactic activity accounts for accumulation of polymorphonuclear leukocytes in cerebrospinal fluid of rabbits with pneumococcal meningitis (1984)

Experiments were performed to identify the chemoattractant for polymorphonuclear leukocytes that appears in the cerebrospinal fluid of rabbits with experimental pneumococcal meningitis. Meningitis was induced in anesthetized New Zealand white rabbits by injecting 10(4) cells of stationary-phase Streptococcus pneumoniae type III intracisternally. Before bacteria were injected, cerebrospinal fluid contained neither polymorphonuclear leukocytes nor chemotactic activity. Significant chemotactic activity for rabbit polymorphonuclear leukocytes was detected 12 h after inoculation with bacteria and was maximal after 18 to 20 h. Chemotactic activity appeared in cerebrospinal fluid while concentrations of pneumococci and total protein were increasing but before there was any accumulation of polymorphonuclear leukocytes. The chemotactic activity in cerebrospinal fluid was heat stable (56 degrees C for 30 min), eluted from Sephadex G-75 with a profile identical to that of the chemotactic activity in zymosan-activated rabbit serum, and was inhibited by treatment with antibodies to native human C5. In addition, preincubation of polymorphonuclear leukocytes with partially purified rabbit C5a selectively inhibited their subsequent chemotactic responses to cerebrospinal fluid. These data indicate that complement (C5)-derived chemotactic activity appears in cerebrospinal fluid during the course of experimental pneumococcal meningitis in rabbits and suggest that this activity accounts for the accumulation of polymorphonuclear leukocytes observed in this infection.