gebhart/patrepeval huggingface dataset:
Genetic Engineering of an Ectomycorrhizal Fungus Laccaria bicolor for Use as a Biological Control Agent (1999)

We are developing genetically engineered ectomycorrhizal fungi for use as biological control agents. Using the gold particle bombardment technique, we transformed Laccaria bicolor, an ectomycorrhizal fungus capable of forming ectomycorrhizae on a wide range of plant hosts. Structural gene sequences of the hygromycin B phosphotransferase (hph) gene (hygromycin resistance for selection of transformants), β-glucuronidase (GUS) gene (reporter gene for tracking transformants) and Bacillus thuringiensis δ-endotoxin (BtCryIIIA) gene (Coleopteran specific insecticidal gene from Bacillus thuringiensis var tenebrionis) were introduced into L. bicolor simultaneously. Southern and western blot analyses showed that the introduced sequences were inserted into the fungal genome and transcribed and translated. One of the transformants produced the insecticidal toxin; however, the levels were not sufficient to perform toxicity assays. The technique developed can be employed to generate superior strains of L. bicolor suitable for use as biological control agents in various soil and plant conditions.
Roadmap: Tough times ahead as technology gap widens (2006)

Logic designers may have to become even more intimate with the vagaries of manufacturing processes unless new techniques are found, claims researchers.
Integration of offshore wind farm with multiple oil and gas platforms (2011)

This paper discusses stability and control issues for an isolated offshore system consisting of a wind farm and five oil and gas platforms.
Catalysts for silylations with 1,1,1,3,3,3-hexamethyldisilazane (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalysts for silylations with 1,1,1,3,3,3-hexamethyldisilazaneCornelis A. Bruynes and Theodorus K. JurriensCite this: J. Org. Chem. 1982, 47, 20, 3966–3969Publication Date (Print):September 1, 1982Publication History Published online1 May 2002Published inissue 1 September 1982https://pubs.acs.org/doi/10.1021/jo00141a031https://doi.org/10.1021/jo00141a031research-articleACS PublicationsRequest reuse permissionsArticle Views2571Altmetric-Citations138LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Generic Implementation of a Distress Sound Extraction System for Elder Care (2006)

Medical remote monitoring at home is an alternative to improve the patient's comfort, to detect distress situation rapidly and reduce hospitalization costs. Physiologic and position sensors give already numerous information, but sound classification can give interesting additional information. A real-time implementation of a smart sound system capable of detecting and identifying sound events in noisy conditions is presented in this paper. The advantage of this implementation is the use of a generic PC station: the hardware requirements are only a sound card, a microphone and an Internet link used to transmit alarm. In the case of an alarm, the information can be sent through network to a remote monitoring center and/or to a close person by email or SMS. The system is composed of 2 modules: detection and classification. The event detection module is carried out in real time in order to extract possible alarm sounds. The sound classification module is runs in a parallel task; it carries out a first segmentation between sound and speech. In the speech case, a speech recognition system is launched (not described in this paper) and in the sound case, a classification between predefined classes is carried out.
A new quadrupole PPM focusing scheme for high density electron beams (2002)

The paper discusses a new periodic focusing scheme employing periodic quadrupole magnets. The basic mechanism of focusing is explained as well as the basic dynamics of high density beams subject to a periodic quadrupole field. The main advantage appears to be small demagnetization effects in the quadrupole magnets, so that a larger focusing field is available. Furthermore, since pole pieces are not required, saturation problems are absent. The quadrupole scheme is therefore expected to have merit predominantly for focusing high power beams at extremely high frequencies where dimensions are minute.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Extracting text from WWW images (2002)

The authors examine the problem of locating and extracting text from images on the World Wide Web. They describe a text detection algorithm which is based on color clustering and connected component analysis. The algorithm first quantizes the color space of the input image into a number of color classes using a parameter-free clustering procedure. It then identifies text-like connected components in each color class based on their shapes. Finally, a post-processing procedure aligns text-like components into text lines. Experimental results suggest this approach is promising despite the challenging nature of the input data.
Identification of the tyrosine phosphatase PTP1C as a B cell antigen receptor-associated protein involved in the regulation of B cell signaling. (1995)

Recent data implicating loss of PTP1C tyrosine phosphatase activity in the genesis of the multiple hemopoietic cell defects found in systemic autoimmune/immunodeficient motheaten (me) and viable motheaten (mev) mice suggest that PTP1C plays an important role in modulating intracellular signaling events regulating cell activation and differentiation. To begin elucidating the role for this cytosolic phosphatase in lymphoid cell signal transduction, we have examined early signaling events and mitogenic responses induced by B cell antigen receptor (BCR) ligation in me and mev splenic B cells and in CD5+ CH12 lymphoma cells, which represent the lymphoid population amplified in motheaten mice. Despite their lack of functional PTP1C, me and mev B cells proliferated normally in response to LPS. However, compared with wild-type B cells, cells from the mutant mice were hyperresponsive to normally submitogenic concentrations of F(ab')2 anti-Ig antibody, and they exhibited reduced susceptibility to the inhibitory effects of Fc gamma IIRB cross-linking on BCR-induced proliferation. Additional studies of unstimulated CH12 and wild-type splenic B cells revealed the constitutive association of PTP1C with the resting BCR complex, as evidenced by coprecipitation of PTP1C protein and phosphatase activity with BCR components and the depletion of BCR-associated tyrosine phosphatase activity by anti-PTP1C antibodies. These results suggest a role for PTP1C in regulating the tyrosine phosphorylation state of the resting BCR complex components, a hypothesis supported by the observation that PTP1C specifically induces dephosphorylation of a 35-kD BCR-associated protein likely representing Ig-alpha. In contrast, whereas membrane Ig cross-linking was associated with an increase in the tyrosine phosphorylation of PTP1C and an approximately 140-kD coprecipitated protein, PTP1C was no longer detected in the BCR complex after receptor engagement, suggesting that PTP1C dissociates from the activated receptor complex. Together these results suggest a critical role for PTP1C in modulating BCR signaling capacity, and they indicate that the PTP1C influence on B cell signaling is likely to be realized in both resting and activated cells.
Probiotic potential of Enterococcus faecalis strains isolated from meconium (2015)

107 bacterial isolates with Gram positive staining and negative catalase activity, presumably assumed as lactic acid bacteria, were isolated from samples of meconium of 6 donors at Roubaix hospital, in the north of France. All these bacterial isolates were identified by MALDI-TOF mass spectrometry as Enterococcus faecalis. However, only six isolates among which E. faecalis 14, E. faecalis 28, E. faecalis 90, E. faecalis 97, and E. faecalis 101 (obtained from donor 3), and E. faecalis 93 (obtained from donor 5) were active against some Gram-negative bacteria and Gram-positive bacteria , through production of lactic acid, and bacteriocin like inhibitory substances. The identification of these isolates was confirmed by 16rDNA sequencing and their genetic relatedness was established by REP-PCR and pulsed field gel electrophoresis methods. Importantly, the aforementioned antagonistic isolates were sensitive to various classes of antibiotics tested, exhibited high scores of coaggregation and hydrophobicity, and were not hemolytic. Taken together, these properties render these strains as potential candidates for probiotic applications.
Software Tools for Analysis of Mass Spectrometric Lipidome Data (2006)

New software tools for quantitative analysis of mass spectrometric lipidome data have been developed. The LIMSA tool finds and integrates peaks in a mass spectrum, matches the peaks with a user-supplied list of expected lipids, corrects for overlap in their isotopic patterns, and quantifies the identified lipid species according to internal standards. Three different algorithms for isotopic correction (deconvolution) were implemented and compared. LIMSA has a convenient user interface and can be applied on any type of MS spectrum. Typically, analysis of one spectrum takes only a few seconds. The SECD tool, designed for analysis of LC-MS data sets, provides an intuitive and informative display of MS chromatograms as two-dimensional "maps" for visual inspection of the data and allows the user to extract mass spectra, to be further analyzed with LIMSA, from arbitrary regions of these maps. More reliable analysis of complex lipidome data with improved signal-to-noise ratio is obtained when compared to standard time-range averaged spectra. The functionality of these tools is demonstrated by analysis of standard mixtures as well as complex biological samples. The tools described here make accurate, high-throughput analysis of extensive sample sets feasible and are made available to the scientific community free of charge.
The administration of drugs during resuscitation. (2007)

There are a few drugs that are indicated during the initial management of a cardiac arrest. They can be considered once defibrillation has been performed (if indicated) and CPR has started.
Analysis of insertion-deletion from deep-sequencing data: software evaluation for optimal detection (2012)

Insertion and deletion (indel) mutations, the most common type of structural variance in the human genome, affect a multitude of human traits and diseases. New sequencing technologies, such as deep sequencing, allow massive throughput of sequence data and greatly contribute to the field of disease causing mutation detection, in general, and indel detection, specifically. In order to infer indel presence (indel calling), the deep-sequencing data have to undergo comprehensive computational analysis. Selecting which indel calling software to use can often skew the results and inherent tool limitations may affect downstream analysis. In order to better understand these inter-software differences, we evaluated the performance of several indel calling software for short indel (1-10 nt) detection. We compared the software's sensitivity and predictive values in the presence of varying parameters such as read depth (coverage), read length, indel size and frequency. We pinpoint several key features that assist successful experimental design and appropriate tool selection. Our study may also serve as a basis for future evaluation of additional indel calling methods.
Alternative transcription factor sigmaSB of Staphylococcus aureus: characterization and role in transcription of the global regulatory locus sar (1997)

A homolog of the multiple-stress-responsive transcription factor sigmaB of Bacillus subtilis was predicted from the DNA sequence analysis of a region of the Staphylococcus aureus chromosome. A hybrid between the coding sequence of the first 11 amino acids of the gene 10 leader peptide of phage T7 (T7.Tag) and the putative sigB gene of S. aureus was constructed and cloned into Escherichia coli BL21(DE3)pLysS for overexpression from a T7 promoter. A homogeneous preparation of the overproduced protein was obtained by affinity chromatography with a T7.Tag monoclonal antibody coupled to agarose. The amino-terminal amino acid sequence of the first 22 residues of the purified protein matched that deduced from the nucleotide sequence. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified protein, designated sigmaSB, indicated that it migrated as an approximately 39-kDa polypeptide. Promoter-specific transcription from the B. subtilis sigmaB-dependent PB promoter of the sigB operon was stimulated by sigmaSB in a concentration-dependent fashion when reconstituted with the S. aureus core RNA polymerase (RNAP). Specific transcript from the predicted sigmaB-dependent PB promoter of the sigB operon of S. aureus was obtained by the reconstituted RNAP in a runoff transcription reaction. The sar operon of S. aureus contains three promoter elements (P1, P2, and P3) and is known to partly control the synthesis of a number of extracellular toxins and several cell wall proteins. Our in vitro studies revealed that transcription from the P1 promoter is dependent on the primary sigma factor sigmaSA, while that of the P3 promoter is dependent on sigmaSB. As determined by primer extension studies, the 5' end of the sigmaSB-initiated mRNA synthesized in vitro from the sar P3 promoter is in agreement with the 5' end of the cellular RNA.
Foundations and Technological Landscape of Cloud Computing (2013)

The cloud computing paradigm has brought the benefits of utility computing to a global scale. It has gained paramount attention in recent years. Companies are seriously considering to adopt this new paradigm and expecting to receive significant benefits. In fact, the concept of cloud computing is not a revolution in terms of technology; it has been established based on the solid ground of virtualization, distributed system, and web services. To comprehend cloud computing, its foundations and technological landscape need to be adequately understood. This paper provides a comprehensive review on the building blocks of cloud computing and relevant technological aspects. It focuses on four key areas including architecture, virtualization, data management, and security issues.
Determination of a 12 kb nucleotide sequence around the 76° region of the Bacillus subtilis chromosome (1996)

The nucleotide sequence of a 12361 bp DNA segment in the 76 degrees region of the Bacillus subtilis chromosome has been determined. Ten putative ORFs were identified. The deduced amino acid sequences of the products of two of them (glv-1 and glv-2) exhibited high similarity to those of glvG (6-phospho-beta-glucosidase gene) and glvC [permease (the IIC domain) of the phosphotransferase system (PTS)], respectively, in the glv operon of Escherichia coli. The C-terminal region of Glv-2 exhibited similarity to the entire region of GlvB (the IIB domain of PTS) of E. coli, suggesting fusion of the glvC and glvB genes in B. subtilis. glv-1, yfiA and glv-2 seem to form an operon of a phosphoenolpyruvate:sugar PTS, followed by a presumed four-membered operon of an ABC transport system. Moreover, a presumed sugar symporter and its regulatory genes were located in this region.
Universal state inversion and concurrence in arbitrary dimensions (2001)

Wootters [Phys. Rev. Lett. 80, 2245 (1998)] has given an explicit formula for the entanglement of formation of two qubits in terms of what he calls the concurrence of the joint density operator. Wootters's concurrence is defined with the help of the superoperator that flips the spin of a qubit. We generalize the spin-flip superoperator to a ``universal inverter,'' which acts on quantum systems of arbitrary dimension, and we introduce the corresponding generalized concurrence for joint pure states of ${D}_{1}\ifmmode\times\else\texttimes\fi{}{D}_{2}$ bipartite quantum systems. We call this generalized concurrence the I concurrence to emphasize its relation to the universal inverter. The universal inverter, which is a positive, but not completely positive superoperator, is closely related to the completely positive universal-NOT superoperator, the quantum analogue of a classical NOT gate. We present a physical realization of the universal-NOT superoperator.
Atomic Layer Deposition Functionalized Composite SOFC Cathode La<sub>0.6</sub>Sr<sub>0.4</sub>Fe<sub>0.8</sub>Co<sub>0.2</sub>O<sub>3-δ</sub> -Gd<sub>0.2</sub>Ce<sub>0.8</sub>O<sub>1.9</sub>: Enhanced Long-Term Stability (2013)

We report that the long-term stability of a conventional mixed oxide-ion and electron conducting solid oxide fuel cell cathode, La0.6Sr0.4Fe0.8Co0.2O3−δ-Gd0.2Ce0.8O1.9 (LSCF-GDC) composite, can be markedly improved by functionalizing its surfaces with a conformal layer of nanoscale ZrO2 films through atomic layer deposition (ALD). Over a >1100 h testing at 800 °C, the overcoated LSCF-GDC cathode exhibited respective polarization and ohmic area-specific-resistances 3 and 1.5 times lower than the pristine sample, whereas the pristine LSCF-GDC cathode degraded at a rate 4 times faster than the overcoated one. The multifunctionality of porosity, mixed conductivity, and suppressed Sr-enrichment enabled by the nanoscaled ALD-ZrO2 overcoats are attributed to the performance retention observed for the overcoated cathode.
Sensitive and label-free biosensing of RNA with predicted secondary structures by a triplex affinity capture method (2012)

A novel biosensing approach for the label-free detection of nucleic acid sequences of short and large lengths has been implemented, with special emphasis on targeting RNA sequences with secondary structures. The approach is based on selecting 8-aminoadenine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating a stable triplex-stranded helix at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. With this strategy, we have detected short DNA sequences (32-mer) and purified RNA (103-mer) at the femtomol level in a few minutes in an easy and level-free way. This approach is particularly suitable for the detection of RNA molecules with predicted secondary structures, reaching a limit of detection of 50 fmol without any label or amplification steps. Our methodology has shown a marked enhancement for the detection (18% for short DNA and 54% for RNA), when compared with the conventional duplex approach, highlighting the large difficulty of the duplex approach to detect nucleic acid sequences, especially those exhibiting stable secondary structures. We believe that our strategy could be of great interest to the RNA field.
The Estimation of Personalized HRTFs in Individual VAS (2008)

The synthesis of individual virtual auditory system (VAS) is an important and state-of-the-art technology in virtual reality. One of the key factors for individual VAS is to obtain a set of proper head related transfer functions (HRTFs). A personalization method is presented in this paper. First, multiple linear regression analysis is applied to get the linear relationship between HRTFs and some anthropometric parameters; second, the magnitude response of the individual HRTF is estimated; finally the center frequencies of the two prominent notches in the estimated magnitude response are modified by using the center frequencies estimated from the subject's pinna parameters. The results show that the modified HRTF is more approximate to the measured one.
Diffusion and outdiffusion of aluminium implanted into silicon (1993)

The diffusion of aluminium in single-crystal silicon has been studied in the temperature range 1000-1290 degrees C. A low implantation dose (1*1014 Al cm-2) was used to avoid dopant precipitation and a high energy (6.0 MeV) to reduce the influence, if any, of the surface. The experimental profiles, measured by spreading resistance, have been fitted taking into account the escape of Al from the Si surface into the ambient atmosphere. A pre-exponential value of 8.88 cm2 s-1 and an activation energy of 3.44 eV have been found in this experiment. When aluminium is implanted through a capping layer, a reduction of the residual amount has been observed at medium energy (300 keV), while no difference has been measured at high energy (6 MeV).
On the buffer dynamics of scalable video streaming over wireless network (2005)

The 3G mobile standard offers an unprecedented high speed digital service that can carry multimedia traffic such as video streaming. The distributed system buffers in the conventional client-server architecture absorb the fluctuations of channel conditions such as bit rate variations, delays and jitters. To enable easy control of the 3G system, the MPEG committee is developing scalable video coding technology that provides flexibility for transmission over wireless networks. This paper address issues in controlling these buffers that can impact and benefit the end-to-end system performance using the novel scalable video technology. Specifically, we explore the effects of diverse channel characteristics on the buffers performance tolerance, and its impact on the quality of service. A novel model-based adaptive media playout (AMP) buffer control is proposed to mitigate the risks of buffer outage.
Effects of nasal continuous positive airway pressure on apnoea index and sleep in infants (1995)

We conclude that nasal CPAP is an effective treatment for infantile apnoea. Sleep apnoea in these infants is associated with profound sleep fragmentation, which is reversed by nasal CPAP.
Detecting and Tracking Spatio-temporal Clusters with Adaptive History Filtering (2008)

This paper addresses the problem of detecting and tracking moving clusters in spatio-temporal data sets. Spatio-temporal data sets contain data elements that move in space over time. Traditional data clustering algorithms work well on static data sets that contain well separated clusters. When traditional techniques are applied to spatio-temporal data they breakdown when the moving data elements intersect the space occupied by elements from another cluster. The goal of this work is to improve the accuracy of traditional data clustering algorithms on spatio-temporal data sets. Many clustering algorithms create clusters based on the distance between the elements. We extend this distance measure to be a function of the position history of the elements. We show through a series of experiments that the use of the history based distance measures greatly improves the performance of existing data clustering algorithms on spatio-temporal data sets. In random data sets we achieve up to a 90% improvement in cluster accuracy. To evaluate the clustering algorithms we created 102 spatio-temporal data sets. We also defined a set of metrics that are used to evaluate the performance of the clustering algorithms on the spatio-temporal data sets.