gebhart/patrepeval huggingface dataset:
Zebrafish Tumor Assays: The State of Transplantation (2009)

Tumor transplant studies are important tools for studying cancer biology in a model organism. Transplantation is especially important for assaying tumor cell malignancy and migration capabilities, and is critical for identifying putative cancer stem cell populations. In this review, we discuss the current state of tumor transplantation studies performed in the zebrafish. We address several zebrafish-specific considerations for development of the transplant assay, including choosing recipient animals, transplant methods, and post-transplant observation. We also examine how the zebrafish is an advantageous model for transplantation, particularly with development of the translucent fish. Transplantation has already been critical for characterizing zebrafish models of leukemia, rhabdomyosarcoma, and melanoma. With further development of imaging techniques and other tools, zebrafish tumor transplantation will continue to contribute to our understanding of tumor cell biology.
Response Surface Optimization and Kinetics of Isolating Chitin from Pink Shrimp (<i>Solenocera melantho</i>) Shell Waste (1997)

Response surface methodology has been applied to the isolation of chitin from pink shrimp shell powder. The optimal deproteinization condition occurs at 75 °C, 2.5 N NaOH. A minimal solution to solid ratio of 5 mL/g is required to maintain fluidity during deproteinization. Deproteinization exhibits two-stage first-order reaction kinetics. The maximum deproteinization rate constant approaches 0.1 min-1 when the protein content is decreased from 16% to slightly above 7%. After the first 30 min, the deproteinization rate constant could decrease up to 2 orders of magnitude. The optimal demineralization condition is around 1.7 N HCl, with an acid solution to solid ratio of 9 mL/g at ambient temperature. Demineralization could be described as a pseudo-first-order reaction. The demineralization rate constant ranges from 0.00020 to 0.017 min-1. Keywords: Chitin; deproteinization; demineralization; optimization; kinetics
Two-particle quantum interference in tunnel-coupled optical tweezers (2014)

The quantum statistics of atoms is typically observed in the behavior of an ensemble via macroscopic observables. However, quantum statistics modifies the behavior of even two particles. Here, we demonstrate near-complete control over all the internal and external degrees of freedom of two laser-cooled (87)Rb atoms trapped in two optical tweezers. This controllability allows us to observe signatures of indistinguishability via two-particle interference. Our work establishes laser-cooled atoms in optical tweezers as a promising route to bottom-up engineering of scalable, low-entropy quantum systems.
Segmented Reinforcement Variable Stiffness Materials for Reconfigurable Surfaces (2010)

Reconfigurable and morphing structures may provide a range of new functionalities such as optimization over broad operational conditions and multi-mission capability. This article introduces a new generic approach to achieving large strains in materials with high elastic moduli (5-30 GPa). The work centers on creating variable stiffness composite materials which exhibit a controllable change in elastic modulus (bending or axial) and large reversible strains (5-15%). We have performed a simulation study to better understand the implication of various geometric design parameters on the elastic and deformation behavior. Using this information, a series of prototype materials were prepared using a commercial shape memory polymer, and measurements on these materials indicate a controllable change in stiffness as a function of temperature with large reversible strain accommodation. We have fabricated and tested several design variations of laminar morphing materials which exhibit structural stiffness values of 8-12 GPa, changes in modulus of 15-77x and large reversible axial of 2-10%. Results indicate that significant controllable changes in stiffness are possible. Further, agreement between simulations and prototype material properties indicate that simulations may be used an effective screening tool to specify micromechanical design variations for specific application requirements.
Motion estimation for H.264/AVC on multiple GPUs using NVIDIA CUDA (2009)

To achieve the high coding efficiency the H.264/AVC standard offers, the encoding process quickly becomes computationally demanding. One of the most intensive encoding phases is motion estimation. Even modern CPUs struggle to process high-definition video sequences in real-time. While personal computers are typically equipped with powerful Graphics Processing Units (GPUs) to accelerate graphics operations, these GPUs lie dormant when encoding a video sequence. Furthermore, recent developments show more and more computer configurations come with multiple GPUs. However, no existing GPU-enabled motion estimation architectures target multiple GPUs. In addition, these architectures provide no early-out behavior nor can they enforce a specific processing order. We developed a motion search architecture, capable of executing motion estimation and partitioning for an H.264/AVC sequence entirely on the GPU using the NVIDIA CUDA (Compute Unified Device Architecture) platform. This paper describes our architecture and presents a novel job scheduling system we designed, making it possible to control the GPU in a flexible way. This job scheduling system can enforce real-time demands of the video encoder by prioritizing calculations and providing an early-out mode. Furthermore, the job scheduling system allows the use of multiple GPUs in one computer system and efficient load balancing of the motion search over these GPUs. This paper focuses on the execution speed of the novel job scheduling system on both single and multi-GPU systems. Initial results show that real-time full motion search of 720p high-definition content is possible with a 32 by 32 search window running on a system with four GPUs.
2012 Annual Global Tax Competitiveness Ranking – A Canadian Good News Story (2012)

Since 2000, Canada has been remarkably successful in building a more competitive corporate tax system, principally by lowering tax rates and broadening the tax base. Canada’s marginal effective tax rate (METR) is now the lowest, and hence the most tax-competitive among the G-7, the 20th most tax-competitive in the 34-member OECD, and 57th among the 90 countries surveyed in this paper. The result has been greater investment and improved economic growth despite recessionary pressures. In particular, provincial sales tax harmonization with the GST has heightened Ontario’s competitiveness and promises to do the same for PEI, the latest convert to the cause. However, progress has not been uniform. Some provincial governments have lost focus by raising rates or introducing tax preferences that narrow the base, inevitably harming business efficiency. British Columbia’s decision to replace the new Harmonized Sales Tax with the old retail sales tax will cost it dearly, especially when it comes to public spending. On the other hand, corporate tax rate reductions of more than 30 percent (since 2000) have, contrary to the critics’ cries, failed to make an appreciable dent in tax revenues thanks to multinationals’ habit of shifting profits to Canada to take advantage of lower rates. This paper, in providing a candid snapshot of Canadian taxation measured against 89 other nations, serves as an invaluable foundation for understanding how far this country has come, and what its next steps should be.
Unified charge control model and subthreshold current in heterostructure field-effect transistors (1990)

A unified analytical charge control model covering the entire range of gate voltages from below and above threshold is developed for heterojunction field-effect transistors (HFETs). This model is based on a new interpretation of the quantized energy levels for the two-dimensional electron gas. It reduces to a classical charge sheet model in the limit of low surface field. The model is used to interpret the experimental data for the subthreshold regime of HFETs. The results indicate wide range variation of the effective acceptor concentration after device fabrication processing in the unintentionally doped GaAs buffer layer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Synthesis of colloidal nanoparticles during femtosecond laser ablation of gold in water (2003)

Femtosecond laser radiation has been used to ablate a gold target in pure deionized water to produce colloidal gold nanoparticles. We report evidence for two different mechanisms of material ablation in the liquid environment, whose relative contributions determine the size distribution of the produced particles. The first mechanism, associated with thermal-free femtosecond ablation, manifests itself at relatively low laser fluences F&lt;400 J/cm2 and leads to very small (3–10 nm) and almost monodispersed gold colloids. The second one, attributed to the plasma-induced heating and ablation of the target, takes place at high fluences and gives rise to a much larger particle size and broad size distribution. The fabricated nanoparticles exhibit plasmon-related optical absorption peak and are of significance for biosensing applications.
Assessment of the Variation Associated with Repeated Measurement of Gastrointestinal Transit Times and Assessment of the Effect of Oral Ranitidine on Gastrointestinal Transit Times Using a Wireless Motility Capsule System in Dogs (2012)

This study aimed to evaluate the variation associated with repeated measurement of gastrointestinal (GI) transit times and the effect of oral ranitidine on GI transit times in healthy dogs using a wireless motility capsule (WMC) system. Eight privately owned healthy adult dogs were enrolled, and one developed diarrhea and was removed from the study. For the first 3 repetitions, each dog was fed a standard meal followed by oral administration of a WMC. For the 4th repetition, each dog was given ranitidine hydrochloride (75 mg PO every 12 hours) prior to and during assessment of GI transit times. Mean between-subject coefficients of variation for gastric emptying time (GET), small and large bowel transit time (SLBTT), and total transit time (TTT) were 26.9%, 32.3%, and 19.6%, respectively. Mean within-subject coefficients of variation for GET, SLBTT, and TTT were 9.3%, 19.6%, and 15.9%, respectively. Median GET, SLBTT, and TTT without ranitidine were 719, 1,636, and 2,735 minutes, respectively. Median GET, SLBTT, and TTT with ranitidine were 757, 1,227, and 2,083 minutes, respectively. No significant differences in GI transit times were found between any of the 4 repetitions. Under these experimental conditions, no significant effects of oral ranitidine on GI transit times were observed.
The synthesis of novel pyrophosphate analogues and their antiviral activities (1989)

Several methods for the preparation of novel substituted methylene-bisphosphonates were examined. A new synthesis of monofluoromethylene-bisphosphonic acid was developed involving the in situ nucleophilic debromination of tetraisopropyl bromofluoromethylenebisphosphonate. The Michael reaction of tetraalkyl ethenylidenebisphosphonates with alkanethiols was shown to be a facile method of preparation of C- (thioalkyl)methyl methylenebisphosphonates. Tetraisopropyl cyclopropane-1,1-bisphosphonate was synthesised via an intramolecular alkylation of a thallium(I) salt. Thallium(I) salts of methylenebisphosphonates were also used in the synthesis of bisphosphonates related to arildone.\n\nThe reaction of trialkyl phosphites with thiophosgene was investigated and it was shown that in THF at low temperature thio- carbonylbisphosphonate was not formed but that phosphorus stabilised ylids were the principal products.\n\nThe bisphosphonates prepared were tested for inhibitory effect against the RNA polymerase of influenza A/X49; bisphosphonates with electron-withdrawing bridge substituents showing most inhibition. Selected bisphosphonates were found to have slight activity against the reverse transcriptase of HTLV-III; the causative agent of AIDS.
Analytical approach to layout generation of datapath cells (2002)

This paper addresses the problem of layout automation of datapath cells. It presents an analytical approach to transistor placement under full custom design style and demonstrates that it can be applied to practical datapath designs. The presented approach is based on a mathematical model which employs a mixed integer linear programming technique. The placement algorithm adopts the custom design techniques commonly used by datapath layout designers in generating hand-crafted designs. An important aspect of the presented method is the efficient management of the complexity of the underlying mathematical model which makes it applicable to real designs. The authors implemented the presented datapath design technique as an experimental software tool running in the industrial environment. The generated layout results are competitive with manual designs provided by experienced layout designers.
Ibrutinib, Obinutuzumab, Idelalisib, and Beyond: Review of Novel and Evolving Therapies for Chronic Lymphocytic Leukemia (2014)

Chronic lymphocytic leukemia (CLL) is a neoplasm resulting from the progressive accumulation of functionally incompetent monoclonal B lymphocytes in the blood, bone marrow, lymph nodes, and spleen. It is the most common leukemia in Western countries and typically occurs in elderly patients. Initial treatment of CLL often includes a first-generation anti-CD20 antibody (rituximab) with chemotherapy and is the current standard of treatment for "younger" old adults (< 70 yrs of age) or older, clinically fit patients. However, because disease progression and drug resistance are inevitable, patients typically die from their disease or treatment-related complications. Improved understanding of the B-cell receptor signaling pathway, which is essential for normal B-cell growth and tumorigenesis, has led to the development of targeted therapies, with improved short-term clinical outcomes. Ibrutinib, obinutuzumab, and idelalisib, three novel agents recently approved by the U.S. Food and Administration for CLL, all have the potential to change the treatment paradigm. In this article, we describe the pathogenesis of CLL and some of its prognostic factors. Emphasis is on the pharmacology, dosing, clinical efficacy, safety, and place of therapy of ibrutinib, obinutuzumab, and idelalisib. Investigational agents that target different parts of the CLL pathogenic pathway are also described.
Phase correction in two integrator loop filters using new variable-phase inverting amplifier (1980)

A novel variable-phase inverting amplifier is given. The proposed inverter has an adjustable phase which depends on the unity-gain bandwidth of only one of the two o.a.s employed in the circuit. Application of the inverter for phase correction in two-integrator loop filters is discussed.
Dendritic Polyglycerols for Biomedical Applications (2009)

The application of nanotechnology in medicine and pharmaceuticals is a rapidly advancing field that is quickly gaining acceptance and recognition as an independent area of research called "nanomedicine". Urgent needs in this field, however, are biocompatible and bioactive materials for antifouling surfaces and nanoparticles for drug delivery. Therefore, extensive attention has been given to the design and development of new macromolecular structures. Among the various polymeric architectures, dendritic ("treelike") polymers have experienced an exponential development due to their highly branched, multifunctional, and well-defined structures. This Review describes the diverse syntheses and biomedical applications of dendritic polyglycerols (PGs). These polymers exhibit good chemical stability and inertness under biological conditions and are highly biocompatible. Oligoglycerols and their fatty acid esters are FDA-approved and are already being used in a variety of consumer applications, e.g., cosmetics and toiletries, food industries, cleaning and softening agents, pharmaceuticals, polymers and polymer additives, printing photographing materials, and electronics. Herein, we present the current status of dendritic PGs as functional dendritic architectures with particular focus on their application in nanomedicine, in drug, dye, and gene delivery, as well as in regenerative medicine in the form of non-fouling surfaces and matrix materials.
Lightning Rates Relative to Tornadic Storm Evolution on 22 May 1981 (1989)

On 22 May 1981, we acquired lightning and Doppler radar data on two tornadic storms in Oklahoma. Cloud-to-ground lightning flash rates were measured with a magnetic direction-finder network, and total flash rates in the vicinity of the mesocyclone were measured with an L-band radar. In both storms, there was no clear relationship between tornado occurrence and ground flash rates of the storm as a whole, but the stroke rate of each storm was highest after it stopped producing tornadoes. For the second storm, we examined both intracloud and cloud-t-ground lightning rates relative to mesocyclone evolution, analyzing the region within 10 km of the mesocyclone core. Our analysis began during initial stages of the mesocyclone core associated with the fourth and strongest of five tornadoes in the storm and continued until all mesocyclone cores in the storm dissipated. During this period, intracloud lightning flash rates reached a peak of almost 14 min−1 approximately 10 min after the peak in cyclonic shear at the 6 km level and at the same time as the peak in cyclonic shear at the 1.5 km level. The peak in intracloud rates also occurred 5–10 min after the peak in the area within 40 and 45 dBZ contours at the 8 km level and at about the same time as the peak in the area within 50 dBZ contours at 8 km and within 40 dBZ at 6 km. However, ground flash rates in the mesocyclone region were usually less than 1 min−1 during periods when intracloud rates were high and were negatively correlated with cyclonic shear at both 1.5 and 6 km. The ground flash rate was the last parameter to peak, approximately 15 min after intracloud lightning and a few minutes after the latest reflectivity area (the area having >55 dBZ at the 1 km level). We suggest that intracloud rates were governed, in part, by particle interactions during the growth in reflectivity at 7–9 km and, in part, by some process associated with the evolution of cyclonic shear at low altitudes. Earlier studies of tornado storms indicate that the evolution of updrafts and downdrafts affects the evolution of both reflectivity and low-altitude cyclonic shear and so, as in previous storm studies, updraft evolution will affect intracloud rates. We suggest that the peaks in ground flash rates resulted from increasing the distance between the main positive and negative charge centers, from the sedimentation of negative charge to lower altitudes, or from the generation or advection of positive charge below the main negative charge. Although these data are from only a single day, consideration of sferics data from previous studies suggests that 1) most tornadic storms (80% or more) have an increase in total flash rates near the time of the tornado, and 2) the increase in total flash rates is often dominated by intracloud flashes.
Occurrence of multi-drug resistant Salmonella typhi in Calcutta. (1992)

Blood and faecal samples were collected from 122 hospitalised patients of Calcútta clinically suspected to have enteric fever, for isolation of S. typhi. It was isolated from 34.4, 4.9 and 4.1 per cent patients by blood culture, stool culture and by both respectively. The in vitro drug susceptibility testing showed that all the isolates were resistant to chloramphenicol, ampicillin and trimethoprim-sulphamethoxazole, but were uniformly susceptible to ciprofloxacin, norfloxacin and furazolidone. In view of the appearance of multi-drug resistant S. typhi in Calcutta, great care should be exercised in the use of newer quinolone derivatives.
INDUCTION OF VASCULAR ADHESION MOLECULES DURING REJECTION OF HUMAN CARDIAC ALLOGRAFTS (1992)

Adhesion of leukocytes to vascular endothelium is a necessary step leading to the migration of cells into underlying tissues. Vascular adhesion molecules regulate this process and may play an important role in graft rejection. Immunocytochemical studies have been used to investigate the expression of vascular adhesion molecules (ICAM-1, PECAM, VCAM-1, and ELAM-1) in normal donor heart (n = 15) and myocardial biopsies from heart transplant patients with acute rejection (n = 15). Sections were also stained with antibodies against endothelium, leukocytes, MHC antigens, and markers of cell activation. In donor heart EN4, vWF, ICAM-1, PECAM, MHC class I--and, to a lesser extent, VCAM-1 and DR antigen--are expressed on arterioles and venules, whereas ELAM-1 and Pal-E are restricted to venules. Expression of Pal-E, VCAM-1, ICAM-1, and DR antigen was increased during rejection. Capillary endothelium normally expresses EN4, ICAM-1, PECAM, MHC class I, and DR antigen but little, if any, VCAM-1 or ELAM-1. During rejection, however, there is an increased expression of all adhesion molecules. This is paralleled by an increased expression of vWF by capillary endothelium. In addition, ICAM-1 like MHC class I antigen is induced on the myocardial membrane and intercalating discs. Endocardium from donor heart expresses EN4, vWF, PECAM, MHC class I, and sometimes Pal-E and ICAM-1, but very little VCAM-1, ELAM-1 or DR antigen. There is an increased expression of Pal-E, ICAM-1, VCAM-1, and DR antigen on endocardium from rejecting heart biopsies. Proliferating Ki-67+ cells and activated T cells expressing the receptor for IL-2 were also found in biopsies during rejection episodes.
Preparation of 2-Thiazolines from (1,2)-Thioamido-Alcohols; DAST as a Useful Reagent (1995)

Diethylamidosulfur trifluoride (DAST) is shown to be a valuable reagent for the ready access to 2-thiazolines 4a-f starting from the corresponding (1,2)-thioamido-alcohols 3a-f. This mild intramolecular cyclization is fast (< 1 h) and occurs at a temperature as low as -78 °C.
D<sub>2</sub> dopamine receptor genotype and cerebrospinal fluid homovanillic acid, 5‐hydroxyindoleacetic acid and 3‐methoxy‐4‐hydroxyphenylglycol in alcoholics in Finland and the United States (1992)

If a genetic association between the D2 dopamine receptor genotype and alcoholism is mediated by altered dopamine function, then a stronger association might be found in alcoholics who are deviant in indices of dopamine function and by comparing alcoholics to nonalcoholics matched for ethnic origin. Therefore, we evaluated the D2/TaqI polymorphism in 29 impulsive violent alcoholic Finns, 17 nonimpulsive violent alcoholic Finns and 36 Finnish controls free of mental disorders, alcoholism and substance abuse. In 37 of the alcoholics, we measured cerebrospinal fluid (CSF) homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) and 3-methoxy-4-hydroxyphenylglycol. There was no relationship between D2/Taq 1 genotype and concentrations of these monoamine metabolites in this group, which exhibits lower CSF HVA and 5-HIAA as compared to controls. There was also no genotypic difference between Finnish alcoholics and nonalcoholic controls. The lack of relationship between D2/Taq1 genotype and HVA concentration was replicated in 24 Caucasian alcoholics in the United States.