gebhart/patrepeval huggingface dataset:
Evaluation of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by Rhodococcus equi in foals (2002)

Intravenous administration of the commercially available hyperimmune plasma was safe, and the product contained high concentrations of anti-R equi antibodies. However, within this limited foal population, the difference in incidence of pneumonia caused by R equi observed between foals that received plasma and control foals was not significant.
The Cell Surface Protein Gene<i>ecm33</i><sup>+</sup>Is a Target of the Two Transcription Factors Atf1 and Mbx1 and Negatively Regulates Pmk1 MAPK Cell Integrity Signaling in Fission Yeast (2009)

The highly conserved fission yeast Pmk1 MAPK pathway plays a key role in cell integrity by regulating Atf1, which belongs to the ATF/cAMP-responsive element-binding (CREB) protein family. We identified and characterized ecm33(+), which encodes a glycosyl-phosphatidylinositol (GPI)-anchored cell surface protein as a transcriptional target of Pmk1 and Atf1. We demonstrated that the gene expression of Ecm33 is regulated by two transcription factors Atf1 and a MADS-box-type transcription factor Mbx1. We identified a putative ATF/CREB-binding site and an RLM1-binding site in the ecm33(+) promoter region and monitored the transcriptional activity of Atf1 or Mbx1 in living cells using a destabilized luciferase reporter gene fused to three tandem repeats of the CRE and six tandem repeats of the Rlm1-binding sequence, respectively. These reporter genes reflect the activation of the Pmk1 pathway by various stimuli, thereby enabling the real-time monitoring of the Pmk1 cell integrity pathway. Notably, the Deltaecm33 cells displayed hyperactivation of the Pmk1 signaling together with hypersensitivity to Ca(2+) and an abnormal morphology, which were almost abolished by simultaneous deletion of the components of the Rho2/Pck2/Pmk1 pathway. Our results suggest that Ecm33 is involved in the negative feedback regulation of Pmk1 cell integrity signaling and is linked to cellular Ca(2+) signaling.
Randomised, Double Blind, Multicentre, Placebo Controlled Study of Sulodexide in the Treatment of Venous Leg Ulcers (2002)

Sulodexide, a highly purified glycosaminoglycan, was investigated for treatment of venous leg ulcers. Patients (n = 235) undergoing local treatment including wound care and compression bandaging, were randomised to receive either sulodexide or matching placebo for three months. Primary study endpoint was complete ulcer healing after 2 months; secondary endpoints were ulcer healing at 3 months and the time-course changes of ulcer areas. The proportion of patients with complete ulcer healing was higher with sulodexide at 2 months (p = 0.018) and 3 months. The "number needed to treat" to obtain one additional patient healed with sulodexide was 7 at 2 months and 5 at 3 months. The changes in ulcer surface area with time were significant for sulodexide only (p = 0.004). Fibrinogen significantly decreased in sulodexide patients (p = 0.006). In conclusion, sulodexide associated with local treatment proved to be effective and well tolerated in the management of venous leg ulcers.
Achieving range-free localization beyond connectivity (2009)

Wireless sensor networks have been proposed for many location-dependent applications. In such applications, the requirement of low system cost prohibits many range-based methods for sensor node localization; on the other hand, range-free localization depending only on connectivity may underutilize the proximity information embedded in neighborhood sensing. In response to the above limitations, this paper presents a range-free approach to capturing a relative distance between 1-hop neighboring nodes from their neighborhood orderings that serve as unique high-dimensional location signatures for nodes in the network. With little overhead, the proposed design can be conveniently applied as a transparent supporting layer for many state-of-the-art connectivity-based localization solutions to achieve better positioning accuracy. We implemented our design with three well-known localization algorithms and tested it in two types of outdoor test-bed experiments: an 850-foot-long linear network with 54 MICAz motes, and a regular 2D network covering an area of 10000 square feet with 49 motes. Results show that our design helps eliminate estimation ambiguity with sub-hop resolution, and reduces localization errors by as much as 35%. In addition, extensive simulations reveal an interesting feature of robustness for our design under unevenly distributed radio propagation path loss, and confirm its effectiveness for large-scale networks.
Adaptive cancelation of motion artifact in photoplethysmyographic blood volume pulse measurements for exercise evaluation (2002)

An adaptive transversal filter was designed to minimize the impact of motion artifact in the measurement of photoplethysmographic Blood Volume pulse (BVP) in an exercising subject. The rationale of the design is introduced and results from off-line testing with signals recorded from an experimental setup are presented.
Mechanism of DNA binding to the gene 5 protein of bacteriophage fd (1984)

A model for the bimolecular complex arising from the interaction of single-stranded DNA with the gene 5 DNA binding protein (G5BP) of bacteriophage fd is proposed on the basis of difference Fourier analyses and the correlation between structural and physicochemical data. The essential DNA binding element is the G5BP dimer which provides two antiparallel DNA binding channels, each constructed from amino acid contributions of both monomers within the pair. These channels display identical bonding environments but opposite polarities as a consequence of the inherent dyad symmetry within each dimer unit. The two channels are separated by 30 A, and each is 10 A wide and 40 A long. We propose that DNA binding is a consequence of two general sets of interactions. Aromatic side chains of G5BP stack upon nucleic acid bases, and the DNA phosphate backbone is bound by a series of appropriately positioned lysyl and arginyl side chains. The DNA conformation is fully extended, and each binding channel can accommodate up to five nucleotides. Only minor conformational changes in the native G5BP structure are required to optimize the binding of DNA. The G5BP-DNA complexation model presented here serves to explain some of the mechanistic features associated with the role this protein plays in the formation of a nucleoprotein helix during the bacteriophage fd life cycle.
Moisture solubility for differently conditioned transformer oils (2001)

It is important to monitor the moisture content of transformer oil in a transformer. One parameter of particular interest is the moisture solubility of transformer oil. It has been reported that transformer oils under different conditions have different solubility. Measurements of solubility for four different types of conditioned oil are presented in this paper: fresh Shell Diala AX oil, lab-aged Shell Diala A oil, Texas Utility used transformer oil, and Ramapo Substation used transformer oil. To avoid the difficulty of achieving full saturation, this paper proposes an alternative method of measuring the moisture solubility in transformer oil using a relative humidity sensor. It utilizes the linearity between the relative humidity of the oil and the moisture content of the oil, to measure the solubility indirectly. The measured results of fresh oil, lab-aged oil, and the Texas Utility oil are very close, and only Ramapo oil shows different sensor response characteristics and solubility.
Analytical Prediction of Chatter Stability in Milling—Part I: General Formulation (1998)

A new analytical method of chatter stability prediction in milling is presented. A general formulation for the dynamic milling system is developed by modeling the cutter and workpiece as multi-degree-of-freedom structures. The dynamic interaction between the milling cutter and workpiece is modeled considering the varying dynamics in the axial direction. The dynamic milling forces are governed by a system of periodic differential equations with delay whose stability analysis leads to an analytical relation for chatter stability limit in milling. The model can be used to determine the chatter free axial and radial depth of cuts without resorting to time domain simulations.
Structural Elucidation of Oxygenated Storage Lipids in Cucumber Cotyledons (1997)

At early stages of germination, a special lipoxygenase is expressed in cotyledons of cucumber and several other plants. This enzyme is localized at the lipid storage organelles and oxygenates their storage triacylglycerols. We have isolated this lipid body lipoxygenase from cucumber seedlings and found that it is capable of oxygenating in vitro di- and trilinolein to the corresponding mono-, di-, and trihydroperoxy derivatives. To investigate the in vivo activity of this enzyme during germination, lipid bodies were isolated from cucumber seedlings at different stages of germination, and the triacylglycerols were analyzed for oxygenated derivatives by a combination of high pressure liquid chromatography, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy. We identified as major oxygenation products triacylglycerols that contained one, two, or three 13S-hydroperoxy-9(Z),11(E)-octadecadienoic acid residues. During germination, the amount of oxygenated lipids increased strongly, reaching a maximum after 72 h and declining afterward. The highly specific pattern of hydroperoxy lipids formed suggested the involvement of the lipid body lipoxygenase in their biosynthesis. These data suggest that this lipoxygenase may play an important role during the germination process of cucumber and other plants and support our previous hypothesis that the specific oxygenation of the storage lipids may initiate their mobilization as a carbon and energy source for the growing seedling.
Targeted delivery of antigen to hamster nasal lymphoid tissue with M-cell-directed lectins (1997)

The nasal cavity of a rodent is lined by an epithelium organized into distinct regional domains responsible for specific physiological functions. Aggregates of nasal lymphoid tissue (NALT) located at the base of the nasal cavity are believed to be sites of induction of mucosal immune responses to airborne antigens. The epithelium overlying NALT contains M cells which are specialized for the transcytosis of immunogens, as demonstrated in other mucosal tissues. We hypothesized that NALT M cells are characterized by distinct glycoconjugate receptors which influence antigen uptake and immune responses to transcytosed antigens. To identify glycoconjugates that may distinguish NALT M cells from other cells of the respiratory epithelium (RE), we performed lectin histochemistry on sections of the hamster nasal cavity with a panel of lectins. Many classes of glycoconjugates were found on epithelial cells in this region. While most lectins bound to sites on both the RE and M cells, probes capable of recognizing alpha-linked galactose were found to label the follicle-associated epithelium (FAE) almost exclusively. By morphological criteria, the FAE contains >90% M cells. To determine if apical glycoconjugates on M cells were accessible from the nasal cavity, an M-cell-selective lectin and a control lectin in parallel were administered intranasally to hamsters. The M-cell-selective lectin was found to specifically target the FAE, while the control lectin did not. Lectin bound to M cells in vivo was efficiently endocytosed, consistent with the role of M cells in antigen transport. Intranasal immunization with lectin-test antigen conjugates without adjuvant stimulated induction of specific serum immunoglobulin G, whereas antigen alone or admixed with lectin did not. The selective recognition of NALT M cells by a lectin in vivo provides a model for microbial adhesin-host cell receptor interactions on M cells and the targeted delivery of immunogens to NALT following intranasal administration.
Characterization of the class III collagen receptor, a phosphorylated, transmembrane glycoprotein expressed in nucleated human cells. (1988)

We previously identified a 90-kDa cell surface glycoprotein, termed the class III collagen receptor (CRIII), that bound to collagen in affinity chromatography experiments (Wayner, E. A., and Carter, W. G. (1987) J. Cell Biol. 105, 1873-1884). Here, we utilize monoclonal antibodies to define three domains of the CRIII, hydrophobic transmembrane, phosphorylated cytoplasmic, and glycosylated extracellular. The domain designations are based on the following characteristics. (i) Differential extraction, phase partitioning with Triton X-114, and incorporation into liposomes all indicate that the CRIII is an intrinsic membrane receptor with a hydrophobic domain. After incorporation into liposomes the CRIII binds collagen. (ii) Immunofluorescence microscopy reveals that most nucleated cells express the CRIII and that after extraction with Triton X-100, the Triton-insoluble CRIII distributes in a fibrillar pattern at the cell periphery and in closed loops that partially co-distributed with vimentin. The CRIII contains phosphoserine residues which are located on a cytoplasmic domain that may interact with the cytoskeleton. (iii) The CRIII contains 25% carbohydrate in 8-10 asparagine-linked carbohydrate chains of 2800 daltons each bound to a 65-kDa core peptide in the extracellular domain. Peptide mapping with trypsin defined a glycosylated 27-kDa extracellular fragment and a phosphorylated and glycosylated 35-kDa transmembrane fragment. These data suggest a model for the CRIII that links the cytoskeleton with the extracellular matrix.
RITUAL: a performance driven placement algorithm (1992)

An algorithm for obtaining a placement of large scale cell-based ICs subject to performance constraints is described. The problem is formulated as a constrained programming problem and is solved in two phases: continuous and discrete. Constraints are placed on total path delays including cell and interconnect delays, and the behavior of all the paths is captured. Mathematical techniques and heuristics based on Lagrangian relaxation are used to find an approximate solution to the constrained problem. The algorithm yields good results, as shown on a set of real examples. On the average, between 8% and 30% improvement in the interconnect delay of these examples is obtained with little or no impact on chip area after routing by modifying the placement alone.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Lytic versus stimulatory synapse in cytotoxic T lymphocyte/target cell interaction: Manifestation of a dual activation threshold (2003)

Activation of biological functions in T lymphocytes is determined by the molecular dynamics occurring at the T cell/opposing cell interface. In the present study, a central question of cytotoxic T lymphocyte (CTL) biology was studied at the single-cell level: can two distinct activation thresholds for cytotoxicity and cytokine production be explained by intercellular molecular dynamics between CTLs and targets? In this study, we combine morphological approaches with numerical analysis, which allows us to associate specific patterns of calcium mobilization with different biological responses. We show that CTLs selectively activated to cytotoxicity lack a mature immunological synapse while exhibiting a low threshold polarized secretion of lytic granules and spike-like patterns of calcium mobilization. This finding is contrasted by fully activated CTLs, which exhibit a mature immunological synapse and smooth and sustained calcium mobilization. Our results indicate that intercellular molecular dynamics and signaling characteristics allow the definition of two activation thresholds in individual CTLs: one for polarized granule secretion (lytic synapse formation) and the other for cytokine production (stimulatory synapse formation).
Genetics of Parkinsonʼs disease (2005)

The combination of genetic, pathologic and molecular findings provide increasing evidence that the pathways identified through the cloning of different disease genes are interacting on different levels and share several major pathogenic mechanisms.
High-yield amplification of<i>Cryptosporidium parvum</i>in interferon γ receptor knockout mice (2008)

To date, large-scale production of Cryptosporidium parvum oocysts has only been achieved by amplification in neonatal calves and sheep. Many laboratories currently depend on supplies from external sources and store oocysts for prolonged periods which results in progressive loss of viability. Six to 8-week-old interferon gamma receptor knockout (IFN gamma R-KO) mice on a C57BL/6 background were inoculated by gavage (2000 oocysts/animal). Fecal pellets were collected daily from 7 days post-infection (p.i.) up to 2 weeks p.i. Intestinal oocyst yield was assessed at days 11, 12 and 14 p.i. by homogenization of intestinal tissues. Ether extraction and one or more NaCl flotations were used to purify oocysts. Total recoveries averaged 2.6 x 10(6) oocysts/mouse from fecal material and 3.8 x 10(7) oocysts/mouse from intestinal tissues. Overall, 2.3 x 10(9) purified oocysts were obtained from 60 mice. Recovered oocysts were capable of sporulation and were shown to be infectious both in vitro and in vivo. Oocyst amplification was achieved in only 11-14 days with minimal expense. The simplicity of this method presents a practical alternative for the routine passage, maintenance and storage of C. parvum in biomedical laboratories.
Anodic oxide films on aluminum (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleAnodic oxide films on aluminumJ. W. Diggle, Thomas C. Downie, and C. W. GouldingCite this: Chem. Rev. 1969, 69, 3, 365–405Publication Date (Print):June 1, 1969Publication History Published online1 May 2002Published inissue 1 June 1969https://pubs.acs.org/doi/10.1021/cr60259a005https://doi.org/10.1021/cr60259a005research-articleACS PublicationsRequest reuse permissionsArticle Views6579Altmetric-Citations1032LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Organometallic compounds of the lanthanides. 59. [1,1'-(3-Oxapentamethylene)dicyclopentadienyl]yttrium and -lutetium 3,5-dimethylpyrazolates. X-ray crystal structure of [O(CH2CH2C5H4)2Ln(.mu.-N2C3HMe2)(.mu.-OH)Ln(C5H4CH2CH2)2O] (Ln = yttrium, lutetium) (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic compounds of the lanthanides. 59. [1,1'-(3-Oxapentamethylene)dicyclopentadienyl]yttrium and -lutetium 3,5-dimethylpyrazolates. X-ray crystal structure of [O(CH2CH2C5H4)2Ln(.mu.-N2C3HMe2)(.mu.-OH)Ln(C5H4CH2CH2)2O] (Ln = yttrium, lutetium)Herbert Schumann, Joerg Loebel, Joachim Pickardt, Changtao Qian, and Zuowei XieCite this: Organometallics 1991, 10, 1, 215–219Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/om00047a052https://doi.org/10.1021/om00047a052research-articleACS PublicationsRequest reuse permissionsArticle Views142Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
100 Gbit/s, 200 km optical transmission experimentusingextremely low jitter PLL timing extraction and all-opticaldemultiplexing based on polarisation insensitive four-wave mixing (1994)

Fully time-division-multiplexed 100 Gbit/s optical transmission is successfully demonstrated through a 200 km fibre, followed by PLL timing extraction and 100 to 6.3 Gbit/s all-optical demultiplexing based on polarisation-insensitive four-wave mixing. The prescaled, 0.3 ps jitter 6.3 GHz clock is recovered from the 100 Gbit/s signal using the four-wave mixing light generated in a polarisation-insensitive 100 GHz laser-diode-amplifier phase detector. Polarisation-insensitive four-wave mixing demultiplexing is achieved by a polarisation rotating loop mirror configuration.
Dielectric behavior of three-phase percolative Ni–BaTiO3/polyvinylidene fluoride composites (2002)

A three-phase percolative composite with a ferroelectric phase (BaTiO3) and metallic inclusions (Ni) embedded into polyvinylidene fluoride matrix was prepared by using a simple blending and hot-molding technique. Effective medium approximations and percolation theory were employed in order to design and describe the dielectric behavior of such three-phase composites. Our experimental results showed that the static dielectric constant of such a three-phase composite can reach above 800 when the Ni concentration is close to its percolation threshold. Such composites have a potential to become capacitors and can be easily fabricated into various shapes due to its flexibility.