gebhart/patrepeval huggingface dataset:
Collagen Mimetic Dendrimers (2002)

The synthesis of single-chain, scaffold (TRIS)- and dendrimer-assembled collagen mimetics (both Gly-Pro-Nleu and Gly-Nleu-Pro sequences) is reported. From the CD spectra and the thermal denaturation studies it can be readily seen that mimetics prepared from the Gly-Nleu-Pro sequence form more thermally stable triple helices than the Gly-Pro-Nleu sequence. Furthermore, the 162-residue collagen mimetic dendrimers exhibit enhanced triple helical stability compared to equivalent scaffold-terminated structures by a substantial increase in the melting temperature in H2O and 2:1 EG/H2O. The concentration dependence for the melting transition was measured which determined that the stabilization effect arises from the intramolecular clustering of the triple helical arrays about the core structure. This ensemble excludes solvent from the interior portion of the array which stabilizes the triple helical bundle.
Sensitive and Selective Detection of Copper Ions with Highly Stable Polyethyleneimine-Protected Silver Nanoclusters (2013)

Copper is a highly toxic environmental pollutant with bioaccumulative properties. Therefore, sensitive Cu(2+) detection is very important to prevent over-ingestion, and visual detection using unaugmented vision is preferred for practical applications. In this study, hyperbranched polyethyleneimine-protected silver nanoclusters (hPEI-AgNCs) were successfully synthesized using a facile, one-pot reaction under mild conditions. The hPEI-AgNCs were very stable against extreme pH, ionic strength, temperature, and photoillumination and could act as sensitive and selective Cu(2+) sensing nanoprobes in aqueous solutions with a 10 nM limit of detection. In addition, hPEI-AgNCs-doped agarose hydrogels were developed as an instrument-free and regenerable platform for visual Cu(2+) and water quality monitoring.
Peroxide model reaction for placental aromatase (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPeroxide model reaction for placental aromatasePhilip A. Cole and Cecil H. RobinsonCite this: J. Am. Chem. Soc. 1988, 110, 4, 1284–1285Publication Date (Print):February 17, 1988Publication History Published online1 May 2002Published inissue 17 February 1988https://pubs.acs.org/doi/10.1021/ja00212a043https://doi.org/10.1021/ja00212a043research-articleACS PublicationsRequest reuse permissionsArticle Views149Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
High resolution subsurface microscopy technique (2002)

The numerical aperture increasing lens (NAIL) technique increases the NA and subsequent resolution while preserving stigmatic imaging, and is therefore a significant improvement over standard optical microscopy for subsurface imaging at the diffraction limit. We give experimental data which demonstrates the NAIL technique capability.
The presence of UDP-N-acetylglucosamine:alpha-3-D-mannoside beta 1,2-N-acetylglucosaminyltransferase I activity in Spodoptera frugiperda cells (IPLB-SF-21AE) and its enhancement as a result of baculovirus infection. (1993)

A Golgi preparation from Spodoptera frugiperda (IPLB-SF-21AE) cells was incubated in the presence of the mannosidase II inhibitor, swainsonine, with the oligosaccharide, M(alpha 1,3)[[M(alpha 1,3)[M(alpha 1,6)]M(alpha 1,6)]] M(beta 1,4)Gn(beta 1,4)Gn (M5Gn2), the preferred substrate for the enzyme, UDP-N-acetylglucosamine:alpha-3-D-mannoside beta 1,2-N-acetylglucosaminyltransferase I (Gn-TI). This resulted in formation of the product, Gn(beta 1,2)M(alpha 1,3)[[M(alpha 1,3)[M(alpha 1,6)]M(alpha 1,6)]]- M(beta 1,4) Gn(beta 1,4)Gn (Gn(I)M5Gn2). A significantly increased (> 4-fold) rate of conversion of M5Gn2 to Gn(I)M5Gn2 occurred with insect cell-derived Golgi preparations that had been infected with a recombinant baculovirus for 66 h, a time at which significant amounts of complex-type oligosaccharides were assembled on a heterologous protein, human plasminogen, expressed in this system. Coupled with previous results (Davidson, D.J., Bretthauer, R.K., and Castellino, F.J. (1991) Biochemistry 30, 9811-9815) that demonstrated the occurrence of virally induced activation of a specific M6-mannosidase in IPLB-SF-21AE cells, it is clear that viral infection of lepidopteran insect cells makes available enzymes that provide and utilize the substrate, M5Gn2-protein. This is a key feature in the explanation of the previous original observations made by this laboratory, that lepidopteran insect cells are capable of assembly of complex-type oligosaccharides on glycoproteins.
Cerebrin prohormone processing, distribution and action in <i>Aplysia californica</i> (2001)

The isolation, characterization, and bioactivity in the feeding circuitry of a novel neuropeptide in the Aplysia californica central nervous system are reported. The 17-residue amidated peptide, NGGTADALYNLPDLEKIamide, has been termed cerebrin due to its primary location in the cerebral ganglion. Liquid chromatographic purification guided by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allowed the isolation of the peptide with purity adequate for Edman sequencing. The cerebrin cDNA has been characterized and encodes an 86 amino acid prohormone that predicts cerebrin and one additional peptide. Mapping using in situ hybridization and immunocytochemistry showed that cerebrin containing neuronal somata are localized almost exclusively in the cerebral ganglion, mostly in the F- and C-clusters. Both immunostaining and mass spectrometry demonstrated the presence of cerebrin in the neurohemal region of the upper labial nerve. In addition, immunoreactive processes were detected in the neuropil of all of the ganglia, including the buccal ganglia, and in some interganglionic connectives, including the cerebral-buccal connective. This suggests that cerebrin may also function as a local signaling molecule. Cerebrin has a profound effect on the feeding motor pattern elicited by the command-like neuron CBI-2, dramatically shortening the duration of the radula protraction in a concentration-dependent manner, mimicking the motor-pattern alterations observed in food induced arousal states. These findings suggest that cerebrin may contribute to food-induced arousal in the animal. Cerebrin-like immunoreactivity is also present in Lymnaea stagnalis suggesting that cerebrin-like peptides may be widespread throughout gastropoda.
Router-scheduler for the modular anatomy of service-oriented automation components (2008)

Automation and production systems are evolving in the direction of autonomous and collaborative components, approaching the idea of an ecosystem. A single habitant of this system is responsible for different and concurrent activities and thus it requires a special adapted anatomy that is balanced for the several requirements. This work introduces an anatomical-like structure for the development of functional and reusable modules of service-oriented automation components. The central attention will be given to their internal structure and the mechanism that bind the modules together, called the Event Router-Scheduler. The resulting software automation components are customized for different tasks due to the inclusion and management of the specialized functional modules and provide the ability to operate in a service-oriented automation and production environment.
Additive neuroprotective effects of 24(S)-hydroxycholesterol and allopregnanolone in an ex vivo rat glaucoma model (2018)

In a rat ex vivo acute glaucoma model, high pressure (75 mmHg) causes swelling of ganglion cell axons and elevates levels of the endogenous steroids 24(S)-hydroxycholesterol (24SH) and allopregnanolone (AlloP). Furthermore, 24SH (0.1 µM) alone elevates AlloP levels via NMDA receptors. With this model, we now investigate possible interactions between 24SH and AlloP. We found that inhibition of AlloP synthesis with dutasteride under high pressure results in severe excitotoxicity in addition to axonal swelling. The excitotoxicity is prevented by exogenous AlloP but not 24SH, indicating that endogenous AlloP is crucial for protection. However, inhibition of 24SH synthesis with voriconazole induces severe excitotoxicity under normal pressure. Paradoxically, the excitotoxicity by voriconazole is better prevented by AlloP than 24SH. These findings suggest that inhibition of 24SH synthesis becomes excitotoxic in the absence of AlloP. We also observed that co-administration of sub-micromolar 24SH (0.1 µM) and AlloP (0.1 µM), concentrations that are only partially effective when administered alone, prevents axonal swelling under high pressure. This apparent enhanced protection indicates strong interaction between the two neurosteroids to preserve neuronal integrity, with 24SH contributing to AlloP synthesis via NMDA receptors and with AlloP playing an essential role in neuroprotection via GABA<sub>A</sub> receptors.
High-average-power harmonic generation (1985)

High-average-power harmonic generation has been limited in the past by the average-power capabilities of the fundamental frequency source. The power densities required for efficient harmonic generation could be reached only by focusing the fundamental in the nonlinear medium. This approach gives rise to large transverse temperature gradients which disrupt phase matching and is inherently not scalable to large apertures. Recent work1 at LLNL has indicated that solid-state lasers may be capable of very high average power. At these high average powers, focusing the beam will result in optical damage to the nonlinear medium.
[Rosacea as a multisystemic disease]. (2004)

Rosacea is a chronic skin disorder, affecting the face and chest, and develops mostly in the 3rd to 6th decades of life. It is characterized by erythema, telangiectasias, and recurrent flushings. During the time of this chronic inflammation, skin typically develops papules, pustules, and swelling. Ocular involvement occurs in 3 to 58% of patients with skin changes. Common ocular signs include blepharoconjunctivitis, meibomitis, and dry eyes. Rosacea keratitis, when present, however, has a poor prognosis and may lead to blindness. Among skin diseases, Helicobacter pylori infection has been often related with rosacea. A higher prevalence of indigestion and Helicobacter pylori infection in rosacea patients than in healthy controls has been reported. However, no causal relation has been identified. On the other hand, oral treatment with metronidazole is beneficial in all of three mentioned manifestations of rosacea (skin, eye, indigestion). There is obvious need for multidisciplinary approach, and investigation to rosacea.
Chemokine Receptor CCR2 Is Not Essential for the Development of Experimental Cerebral Malaria (2003)

Infection with Plasmodium berghei ANKA induces cerebral malaria in susceptible mice. Brain-sequestered CD8(+) T cells are responsible for this pathology. We have evaluated the role of CCR2, a chemokine receptor expressed on CD8(+) T cells. Infected CCR2-deficient mice were as susceptible to cerebral malaria as wild-type mice were, and CD8(+) T-cell migration to the brain was not abolished.
Discovery methods for information extraction (2003)

At the present state of the art, however, performance varies widely depending on the nature of the language being processed and the complexity of the relation being extracted. For restricted sublanguages and simple relations, levels of accuracy comparable to human coders are possible. This has been achieved, for example, for some types of medical records, where both physicians and an extraction system identified diseases with 70-80% accuracy (Friedman et al. 1995). High performance has also been achieved for semi-structured Web documents — documents with some explicit mark-up (Cohen and Jensen 2001). In contrast, for more complex relations and more general texts, accuracies of 50-60% are more typical. Even at these levels IE can be of significant value in situations where the text is too voluminous to be reviewed manually; for example, to provide a document search tool much richer than current keyword systems (Grishman et al 2002). IE is also being used in other applications where perfect recall is not required, such as data mining from text collections and the generation of time lines for texts.
Experimental Evaluation of Effects of Channel Doping on Characteristics of FinFETs (2007)

We investigated channel doping in fin-type double-gate (DG) MOSFETs. We demonstrated through experiments that the threshold voltage was more sensitive to the dopants in the accumulation mode than in the inversion mode. We also found that significant deviation in the threshold voltage from the expected value arose in ultrathin fin-type DG MOSFETs. We attributed this phenomenon to the unexpected dopant loss from the ultrathin channels due to segregation. This finding means that careful doping adjustments must be made in ultrathin-channel devices.
PARADOXIC ELEVATION OF THE LID (1939)

So far as I can ascertain, the case report which follows will in itself be of no practical value to any one. It is presented, however, because no exactly similar phenomenon has been found in the literature and because it has stimulated certain reflections on the movements of the lids. REPORT OF A CASE Beverly L., aged 10 years, was first seen in 1935. At the age of 6 years she had suffered a severe attack of pneumococcic meningitis, after which the left eye had turned out and the upper lid had drooped. Immediately after the illness paralysis of the right leg and arm was present, but this cleared up completely. There were also cerebral changes, resulting in a peculiarly unstable temperament, with extreme restlessness and flight of ideas. The child presented the picture of complete paralysis of the left third nerve, with, in addition, complete paralysis of elevation and
Development of genetic systems for the mycobacteria. (1989)

Requisite to a detailed understanding of the molecular basis of bacterial pathogenesis is a genetic system which allows for the transfer, mutation, and expression of specific genes. Genetic analysis of mycobacteria has been exceedingly difficult since the mycobacteria grow slowly and no natural efficient method of gene transfer within the pathogenic has thus far been found. Using a molecular genetic approach, we have developed both the vectors and the methodology for efficient gene transfer in the mycobacteria. Initially, a novel of type of mycobacteriophage vector was developed, termed a shuttle phasmid. This hybrid shuttle vector replicates in Escherichia coli as a plasmid and in mycobacteria as a phage, capable of introducing foreign DNA into a wide variety of mycobacterial species. A set of shuttle phasmids, constructed from a temperate mycobacteriophage, retained their ability to lysogenize their mycobacterial hosts and could thus introduce foreign DNA stably into mycobacterial cells. An E. coli gene conferring kanamycin-resistance was cloned into these vectors and shown to express in the mycobacteria, thus providing the first selectable marker gene for subsequent genetic studies. Using kanamycin-resistance gene as a selection, the M. fortuitum plasmid pAL5000 replicon, and electroporation; a plasmid transformation system has been developed for both M. smegmatis and BCG. We now plan to use these phage and plasmid systems to analyze, genetically, the virulence attributes of the pathogenic mycobacteria. In addition, by introducing and expressing foreign antigens in BCG, we hope to develop a novel recombinant multi-vaccine vehicle capable of conferring immunity to a variety of bacterial, viral, and parasitic pathogens.
Rapamycin Enhances the Number of Alloantigen-Induced Human CD103+CD8+ Regulatory T Cells In Vitro (2007)

Alloreactive CD103+ CD8+ T(reg) cells may expand and exert their suppressive function during immunosuppressive treatment with rapamycin. These data are relevant in the design of immunosuppressive drug regimens intended to induce and/or maintain transplantation tolerance.
Self-sensing control as applied to a PZT stack actuator used as a micropositioner (1994)

Self-sensing actuation is a control technique that can be applied to many smart materials including piezoceramics. It involves extracting a sensing signal from the actuating material by use of a bridge circuit, then properly feeding this signal back to improve the actuator's performance. The research presented is concerned with the feasibility of applying this technique to a soft PZT stacked actuator used as a micropositioner. The result of applying this control technique was a reduction of the micropositioner's decay time from over 1 ms to under 0.3 ms. A major contributor to the success of this research was the insertion of a nonlinear element in the self-sensing bridge which negated the nonlinear effects of the PZT stack thus enabling an increase in the self-sensing signal-to-noise ratio.
Efficient Content-Based Image Retrieval Method Using Color and Texture (1998)

In this paper, we propose efficient content-based image retrieval methods using the automatic extraction of the low-level visual features as image content. Two new feature extraction methods are presented. The first one is an advanced color feature extraction derived from the modification of Stricker's method. The second one is a texture feature extraction using some DCT coefficients which represent some dominant directions and gray level variations of the image. In the experiment with an image database of 200 natural images, the proposed methods show higher performance than other methods. They can be combined into an efficient hierarchical retrieval method.
Genetic analysis of the <i>Legionella pneumophila</i> DotB ATPase reveals a role in type IV secretion system protein export (2005)

The pulmonary pathogen Legionella pneumophila uses the Dot/Icm type IV secretion system (T4SS) to replicate inside host cells. This apparatus translocates proteins into macrophages to alter their endocytic pathway and enable bacterial growth. Although the secretion ATPase DotB is critical for T4SS function, its specific role in type IV secretion remains undefined. Due to similarity to the VirB11 and PilT ATPases, DotB has been proposed to play a role in assembly of the T4SS, retraction of pili and/or export of substrates. With the goal of understanding the protein's function(s), we isolated and characterized 30 dotB alleles using a variety of phenotypic and biochemical assays. Twenty-four of these alleles possess several dot/icm mutant phenotypes, including a complete lack of intracellular replication, plasmid mobilization and contact-dependent cytotoxicity. These 24 non-functional alleles fall into three classes: those with a known biochemical defect, those with a predicted enzymatic defect and those with an unknown defect. Six other alleles display partial activity in dot/icm phenotypic assays, thus constituting a fourth class. Two mutants in this class are unable to export a subset of T4SS substrates, providing the first evidence for a DotB function in substrate export and suggesting a possible role in substrate selection.
The different roles of charged and neutral atomic and molecular oxidising species in silicon oxidation from ab initio calculations (2001)

We examine the roles of charged and neutral oxidising species based on extensive ab initio DFT calculations. Six species are considered: interstitial atomic O, O−, O2− and molecular species: O2, O2−, O22−. We calculate their incorporation energies into bulk silicon dioxide, vertical electron affinities and diffusion barriers. In our calculations, we assume that the electrons responsible for the change of charge state come from the silicon conduction band, however the generalisation to any other source of electrons is possible and hence our results are also relevant to electron-beam assisted and plasma oxidation. The calculations yield information about the relative stability of oxidising species, and the possible transformations between them and their charging patterns. We discuss the ability to exchange O atoms between the mobile species and the host lattice during diffusion, since this determines whether or not isotope exchange is expected. Our results show very clear trends: (1) molecular species are energetically preferable over atomic ones, (2) charged species are energetically more favourable than neutral ones, (3) diffusion of atomic species (O, O−, O2−) will result in oxygen exchange, whereas the diffusion of molecular species (O2, O2−, O22−) is not likely to lead to significant exchange with the lattice. Our results show thermodynamic trends for oxidising species to capture electrons from Si during oxidation. We identify very different roles for atomic and molecular species and also for different charge states of those species. This points out to opportunities, usually not considered, for optimising thin oxide layers and interface properties for use in electronics devices.
The role of trust in protected mail (1990)

TMail is a privacy-enhanced electronic mail system on a trusted operating system base. A description is given of the TMail cryptographic processes that protect the mail in transit from disclosure, detect modification, and assure source authentication. The trusted operating system is used to protect the mail and the cryptographic modules on the host system. The trust requirements for the system are discussed. Both aspects of the system, cryptography and trust, are needed to assure protection for the mail within the broader system in which it exists.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Design of GPRS System Based on S3C2410A (2011)

The GPRS communication system designed in this paper uses S3C2410A embedded processor and MC39i as the GPRS communication module.This paper designs hardware system,serial driver changes,PPP protocol application porting kit and GPRS communication program etc.,which can be used for wireless data transmission,environmental monitoring and other occasions.