gebhart/patrepeval huggingface dataset:
Probabilistic Latent Variable Models as Nonnegative Factorizations (2008)

This paper presents a family of probabilistic latent variable models that can be used for analysis of nonnegative data. We show that there are strong ties between nonnegative matrix factorization and this family, and provide some straightforward extensions which can help in dealing with shift invariances, higher-order decompositions and sparsity constraints. We argue through these extensions that the use of this approach allows for rapid development of complex statistical models for analyzing nonnegative data.
The Function of Arginine 363 as the Substrate carboxyl‐binding Site in <i>Escherichia coli</i> Serine Hydroxymethyltransferase (1994)

Both the highly conserved Arg363 and Arg372 residues of Escherichia coli serine hydroxymethyltransferase were changed to alanine and lysine residues. Each of the four mutant proteins were purified to homogeneity and characterized with respect to spectral properties of the enzyme-bound pyridoxal phosphate and kinetic properties with substrates and substrate analogs. The R372A and R372 K mutant enzymes exhibited spectra and kinetic properties close to those of the wild-type enzyme. The R363 K mutant enzyme exhibited only 0.03% of the catalytic activity of the wild-type enzyme and a 15-fold reduction in affinity for glycine and serine. The R363A mutant enzyme did not bind serine and glycine and showed no activity with serine as the substrate. Both R363 K and R363A enzymes bound amino acid esters at the active site and catalyzed the retro-aldol cleavage of serine ethyl ester and serinamide. The catalytic activity of the R363 K and R363A enzymes with the serine ethyl ester were about 0.006% and 0.1% of wild-type enzyme activity with serine, respectively. The R363A mutant enzyme catalyzed the half transamination of D-alanine methyl ester and L-alanine methyl ester at rates similar to the rates of transamination of D-alanine and L-alanine by the wild-type enzyme. The results are interpreted to show that R363 is the binding site of the amino acid substrate carboxyl group and that forming an ion pair between R363 and the substrate carboxyl group is an important feature in catalysis by serine hydroxymethyltransferase. Evidence is also provided that R363 may play a role in the substrate-induced open to closed conformational change of the active site.
Molecular mechanisms of metal toxicity and (2001)

Many metals and metal-containing compounds have been identified to be potent mutagens and carcinogens. Recently, a new sub-discipline of molecular toxicology and carcinogenesis has been developed. The combination of newly developed molecular techniques and free radical approach makes it possible to insightfully examine metal-induced carcinogenesis in precise molecular terms so that intricate biological interrelationships can be elucidated. In consideration of the increased amount of new findings deciphered by utilizing these new methods, the 1 st Conference on Molecular Mechanisms of Metal Toxicity and Carcinogenesis was held. In this conference, more than 50 scientists from nine countries presented their novel discoveries concerning metal-induced carcinogenesis, delineated molecular mechanism of metal carcinogenesis, and pro­ posed novel therapeutic intervention and prevention strategies. This article reviews some of the state-of-the-art information presented at the meeting regarding the molecular mechanisms of metal cytotoxicity and carcinogenesis. (Mol Cell Biochem 222: 3-9,2001)
Outcome of Symptomatic Congenital Cytomegalovirus Infection: Results of Long-Term Longitudinal Follow-up (1980)

Thirty-four patients with congenital cytomegalovirus infection who were symptomatic as newborns were followed in a special clinic providing periodic medical and visual examinations as well as psychometric testing and audiometry. All patients had symptoms of congenital infection by 2 weeks of age, and 31 of 34 had virus isolated from urine within the first month of life. Age at latest follow-up varied from 9 months to 14 years with a mean of about 4 years. Ten patients died and 23 surviving patients had adequate follow-up examinations; all but two had evidence of central nervous system or auditory handicaps. Microcephaly was present in 16 (70%), mental retardation in 14 (61%), hearing loss in seven (30%), neuromuscular disorders in eight (35%), and chorioretinitis or optic atrophy in five (22%). Children with symptomatic congenital cytomegalovirus infection are at very high risk for handicaps that will significantly impair development.
The high background immune reactivity of mice to polymorphic determinants on xenogeneic erythrocytes: theoretical and practical implications. (1982)

Background responses have been assessed by fusing lipopolysaccharide- (LPS) stimulated spleen cells from unimmunized mice with MOPC 315.43 myeloma cells and screening the hybrids for the production of antibody against chicken red blood cells (CRBC). Clones specific for CRBC represented about 1% of total hybrid clones (1000 to 5000 clones were obtained per mouse). The majority of the anti-CRBC clones (greater than 95%) secreted antibody against polymorphic CRBC determinants (present on CRBC from some but not all chickens) rather than species-specific determinants present on all CRBC. Some of the polymorphic determinants were linked to the B locus (the MHC of the chicken) and some were non-B antigens. The relative amount of these 2 categories varied slightly according to the mouse strain. These results agree well with the specificities of natural mouse antibody and rosette-forming spleen cells. The response of immunized mice against CRBC and human RBC was also selective for polymorphic determinants. These results have considerable importance for the use of xenogeneic RBC as "standard" antigens, and are interpreted in terms of a model for the advantages of genetic polymorphism as a protection against antigen mimicry by parasites.
Research into bronchitis and emphysema. (1968)

Journal Article Research into bronchitis and emphysema. Get access C M Fletcher, C M Fletcher Royal Postgraduate Medical School, London Search for other works by this author on: Oxford Academic Google Scholar N L Jones, N L Jones Royal Postgraduate Medical School, London Search for other works by this author on: Oxford Academic Google Scholar E J Campbell E J Campbell Royal Postgraduate Medical School, London Search for other works by this author on: Oxford Academic Google Scholar Postgraduate Medical Journal, Volume 44, Issue 507, January 1968, Pages 48–52, https://doi.org/10.1136/pgmj.44.507.48 Published: 01 January 1968
Root Controlled Bridging: A scalable control protocol for Shortest Path Bridging (2008)

Standardization bodies are working on the development of new Carrier Ethernet solutions to remove the current scaling limitations and improve network management. One of them is the IEEE 802.1aq Shortest Path Bridging (SPB), which provides the transmission of unicast and multicast frames on the shortest path in a bridged network. In this paper we propose a novel control protocol for link-state SPB, which can be implemented as an extension to Intermediate System to Intermediate System (IS-IS) routing protocol. We show that our proposal solves scalability problems that arise when a link-state control protocol is applied in SPB. Furthermore, the proposed control protocol assures loop free operation, which is essential for Ethernet networks. We also provide a performance analysis of our new control protocol.
A kinetic-spectrophotometric method for the determination of glucose in solutions (2006)

A kinetic-spectrophotometric method is proposed to determine glucose in solutions. Measurements were performed at 400 nm; the negative peak was obtained by subtracting the absorption spectra of myoglobin (Mb) before and after oxidation. In this method, glucose is added to a mixture of Mb and glucose oxidase. Glucose is oxidized by glucose oxidase and oxygen to gluconate and hydrogen peroxide is generated. The liberated hydrogen peroxide oxidizes the Mb heme (Fe2+) into Fe3+. The higher the glucose concentration added, the more the H2O2 generation, and the more the Mb oxidation (Fe2+ to Fe3+) and, as a result, the higher the absorbance at 400 nm (negative peak, lower absorbance value). The increments of added glucose are monitored by measuring the absorbance decay versus time (0–250 s) at 400 nm. Each glucose concentration has an accompanying unique absorbance value at 250 s. The higher the glucose concentrations, the lower the absorbance at 250 s (measured at 400 nm). The calibration curve for glucose was linear from 0.1 to 3.0 mM; the detection limit was found to be 0.025 mM. There was no interference from major substances present; the only interference was from species that react with H2O2 (ascorbic acid, uric acid, and urea) or that react with glucose (Cu2+ and Fe3+). Standard deviation in the determination was ±0.01 mM for a 1.3 mM glucose solution (n = 10).
Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-α Expression for Microglial Aβ Phagocytosis (2011)

Liver X receptors (LXRs) regulate immune cell function and cholesterol metabolism, both factors that are critically involved in Alzheimer's disease (AD). To investigate the therapeutic potential of long-term LXR activation in amyloid-β (Aβ) peptide deposition in an AD model, 13-month-old, amyloid plaque-bearing APP23 mice were treated with the LXR agonist TO901317. Postmortem analysis demonstrated that TO901317 efficiently crossed the blood-brain barrier. Insoluble and soluble Aβ levels in the treated APP23 mice were reduced by 80% and 40%, respectively, compared with untreated animals. Amyloid precursor protein (APP) processing, however, was hardly changed by the compound, suggesting that the observed effects were instead mediated by Aβ disposal. Despite the profound effect on Aβ levels, spatial learning in the Morris water maze was only slightly improved by the treatment. ABCA1 (ATP-binding cassette transporter 1) and apolipoprotein E (ApoE) protein levels were increased and found to be primarily localized in astrocytes. Experiments using primary microglia demonstrated that medium derived from primary astrocytes exposed to TO901317 stimulated phagocytosis of fibrillar Aβ. Conditioned medium from TO901317-treated ApoE(-/-) or LXRα(-/-) astrocytes did not increase phagocytosis of Aβ. In APP23 mice, long-term treatment with TO901317 strongly increased the association of microglia and Aβ plaques. Short-term treatment of APP/PS1 mice with TO901317 also increased this association, which was dependent on the presence of LXRα and was accompanied by increased ApoE lipidation. Together, these data suggest that astrocytic LXRα activation and subsequent release of ApoE by astrocytes is critical for the ability of microglia to remove fibrillar Aβ in response to treatment with TO901317.
A Study on Cognition and Policy of Shutdown System of Adolescent (2011)

Recently, the side effects from the online game is appearing to adolescent to serious level. This research studied the system to protect adolescent from excessive game usage, the system to limit an online game service connection at the midnight time. Namely we found at a shutdown system introduction of adolescent out about cognition, problems with the game poisoning and effects with shutdown system and alternative policy. The investigation results were as follows. First, only 17.5% of adolescent knew a shutdown system. Second, they were recognizing about the game poisoning seriously. Also the half degree thought them efficiently about a shutdown system introduction. The policy about a shutdown system introduction were as follows. First, public education is activate, and we must keep adolescent from exposing to private education in the entrance examine competition. Second, we need the thing to increase the conversation time of the children with put parents to develop desirable family education program. Lastly, the supply must be achieved with leisure culture contents development of adolescent.
ERYTHROMYCIN. V. ISOLATION AND STRUCTURE OF DEGRADATION PRODUCTS (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTERYTHROMYCIN. V. ISOLATION AND STRUCTURE OF DEGRADATION PRODUCTSPaul F. Wiley, Koert Gerzon, Edwin H. Flynn, Max V. Sigal Jr., and U. Carol QuarckCite this: J. Am. Chem. Soc. 1955, 77, 13, 3677–3678Publication Date (Print):July 1, 1955Publication History Published online1 May 2002Published inissue 1 July 1955https://pubs.acs.org/doi/10.1021/ja01618a092https://doi.org/10.1021/ja01618a092research-articleACS PublicationsRequest reuse permissionsArticle Views91Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A NEW FLAVOR-COATED SACHET BAIT FOR DELIVERING ORAL RABIES VACCINE TO RACCOONS AND COYOTES (2002)

Research was conducted during 1996-2000 to develop baits for delivering an oral rabies vaccine to raccoons (Procyon lotor) and coyotes (Canis latrans). A bait was sought that: (1) was attractive to the target species, (2) could be distributed by aircraft, (3) was as effective (or more so) than the currently used fish meal polymer bait, and (4) could be produced in large numbers by automated procedures and could be purchased by user groups at substantially lower cost. Ten field trials were conducted to document raccoons' bait flavor preferences, evaluate a new vaccine sachet bait coated with various attractants, and determine if the sachet bait would effectively deliver Raboral V-RG oral rabies vaccine (Merial Limited, Athens, Georgia, USA) to this species. Raccoons preferred fish and crustacean-based flavors over those derived from plant materials. Raccoon visits to tracking stations, frequency of bait removals, and percent of sachets discarded by this species that were emptied of placebo vaccine indicated efficacy of the new bait was equal or superior to the currently used fish meal polymer bait. A field trial conducted in fall 1998 compared aerially distributed vaccine-laden sachet and polymer baits and showed there was no difference between the percent of raccoons from the test and reference areas subsequently found positive for rabies antibody. Four bait trials to determine coyote response to sachet baits were conducted in 1997-98. The propensity for canids to gulp or bolt smaller food items is well known. Thus, a first trial involved offering fish-flavored sachet baits of different sizes to 30 captive coyotes to determine if smaller size baits were more frequently swallowed intact. Two field trials were also conducted in fall 1997 to determine if free-ranging coyotes discriminated among sachet baits coated with different attractants. Finally, Raboral V-RG-laden poultry-flavored sachet baits were aerially dropped and the percent of seropositive coyotes was compared with coyotes from surrounding areas where fish meal polymer vaccine baits had been distributed. Captive coyotes did not swallow sachet baits intact, regardless of size. Bait preference field trials indicated that coyotes preferred poultry, cheese/beef tallow, and fish-flavored sachet baits and that such baits were taken at the same rate as polymer baits. A sample of coyotes from the area baited with vaccine-laden sachet baits had a markedly higher (P = 0.01) seropositivity rate than coyotes from areas where vaccine was distributed in polymer baits. Sachet bait production could be facilitated by automated technology and sachet baits used either as an alternative vaccine delivery device or in combination with the fish meal polymer bait.
Tomographic Reconstruction of Ultrasonic Attenuation with Correction for Refractive Errors (1981)

The nonionizing and noninvasive characteristics of ultrasonics promote its increasing use in medical applications. Computerized ultrasonic attenuation tomography is one area where medically significant images may be reconstructed for diagnostic purposes. The objective of this work is to make an initial correction for refraction error and other problems present in computerized ultrasonic tomography. As a refinement of the scanning technique, our method attempts to provide high-quality projection profiles to the reconstruction algorithms. The important aspect of this new scanning mode lies in its correction for refraction in the measured attenuation profiles. In reconstructing phantom targets scanned by our experimental system, results superior to traditional line-of-sight scanning were obtained.
Using shiftable content addressable memories to double memory capacity on embedded systems (2006)

We present a novel algorithm and architecture for memory compression using a series of shiftable content addressable memories (S-CAMs). The main contribution of this new algorithm is the use of a combination of an adaptive shared dictionary used across all memory pages, with one or more local adaptive dictionaries which are flushed after compressing a single memory page. Compression/decompression is capable of handling various types of memory content including application code and data. To this end, a fast compression/decompression architecture is necessary to move code and data from the non-compressed levels of memory hierarchy to the compressed ones and vice versa. Our technique takes advantage of fast parallel comparisons that can be achieved by S-CAMs to implement our dictionary based compression algorithm. Our results show memory reductions that are substantially better (more than doubling the available memory for certain applications) than existing CAM-based memory approaches such as X-Match Pro. These results have been achieved by using the newest embedded processor architectures such as the Xtensa platform that feature a dense instruction word encoding even without compression.
A signal processor for high counting rate gamma ray spectroscopy with NaI(Tl) detectors (1988)

A high-counting-rate signal processor for prompt gamma neutron activation analysis (PGNAA) using NaI(Tl) detectors is described. The specific application is a very-low-rate, high-energy gamma ray in the presence of a high rate at lower energies, producing pile-up at the energy of interest. Several methods for improving counting-rate performance, such as shortening of the detector signal, baseline inspection, pile-up rejection and filtering, have been investigated and implemented. Experimental evaluation shows that the processor allows up to 400000 counts per second without significant distortion of the spectrum due to pile-up and with a minimum degradation of resolution. Through the use of an effective pulse-width-sensitive pile-up rejector, the pile-up effect has been further reduced by a factor of three, while rejecting less than 10% of valid counts. High data through put makes the processor applicable to online process control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Role of B7-CD28/CTLA-4 Costimulation and NF-κB in Allergen-Induced T Cell Chemotaxis by IL-16 and RANTES (2000)

The mechanisms that cause T cell recruitment into inflamed airways of asthmatic individuals are poorly understood. It has been shown previously that both natural exposure to allergen and challenge in the laboratory induce T cell accumulation in the bronchial mucosa of sensitized asthmatics. To study the mechanisms involved in this process, we have used an explant model in which bronchial biopsies taken from mild atopic asthmatic volunteers during fiberoptic bronchoscopy were stimulated in culture for 24 h by the common aeroallergen house dust mite (Dermatophagoides pteronyssinus (Der p)). Analysis of culture supernatants showed that stimulation with Der p significantly enhanced both the generation of T cell chemotactic activity by the mucosal tissue, as assayed in microchemotaxis chambers, and the production of IL-16 and RANTES. Neutralization experiments showed that IL-16 contributed more to the chemotactic activity than RANTES. The fusion protein CTLA-4-Ig, blocking B7:CD28 costimulation, and dexamethasone both significantly reduced the ex vivo production of chemotactic activity and release of IL-16 and RANTES. The proteasome inhibitor Cbz-Ile-Glu(OtBu)-Ala-leucinal also had a significant inhibitory effect on T cell chemotactic activity and IL-16 but not RANTES generation, indicating a role for nuclear factor NF kappa B activation. These results indicate that allergen stimulates cells within the bronchial mucosa to increase IL-16 and RANTES release, both of which contribute to T cell accumulation in asthmatic airways. The allergen-induced chemotactic activity is dependent on cell activation via CD28 and involves, at least partly, NF-kappa B.
Free cholesterol in atherosclerotic plaques: where does it come from? (2007)

These recent findings emphasize the importance of intraplaque hemorrhage as a contributor of free cholesterol in plaques and point to its provocative role in lesion destabilization.
Functional defect in neutrophil cytosols from two patients with autosomal recessive cytochrome-positive chronic granulomatous disease. (1989)

The kinetics of activation of the respiratory burst oxidase in the cell-free oxidase-activating system have been explained by a three-stage mechanism in which the membrane-associated oxidase components M: (a) take up a cytosolic factor S to form a complex M.S that is (b) slowly converted in the second stage to a precatalytic species [M.S]*, which finally (c) takes up two more (possibly identical) cytosolic components, C alpha and C beta, to successively generate [M.S]*C alpha, a low-activity (i.e., high Km) oxidase, and finally [M.S]*C alpha C beta, the ordinary (i.e., low Km) oxidase (Babior, B.M., R. Kuver, and J.T. Curnutte. 1988. J. Biol. Chem. 263:1713-1718). Studies with the cell-free oxidase-activating system from normal neutrophils and from neutrophils obtained from two patients with type II (autosomal recessive cytochrome-positive) chronic granulomatous disease (CGD) have suggested that (a) the defective element in the cytosol from patient neutrophils is S; (b) in normal neutrophil cytosol, S is limiting with respect to M; and (c) C alpha and C beta interact cooperatively with the activated precursor complex [M.S]*. It was further speculated that S might be identical to the nonphosphorylated progenitor of the phosphorylated 48-kD proteins that are missing in certain forms of CGD, and that other forms of type II CGD besides the one described in this report remain to be discovered.