gebhart/patrepeval huggingface dataset:
Structure‐activity relationships of biologically active oligosaccharides (1991)

Abstract. Oligosaccharides that exert biological effects on higher plants (other than as carbon or energy sources) have been termed‘oligosaccharins'. A limited number of specific oligo‐β‐glucans derived from fungal cell walls exhibit oligosaccharin activity, at very low doses, switching on the synthesis of phytoalexins. The structural requirements for this biological activity are stringent, and there is strong evidence that the oligosaccharins of fungal origin act through a receptor in the plant cell membrane. Chemically unrelated oligosaccharins can also be produced by partial digestion of pectins and xyloglucans from higher plant cell walls. In some cases, for example, the α‐ l ‐fucosylated oligoxyloglucans that antagonise the growth‐promoting effect of auxin, these plant‐derived oligosaccharins have relatively strict structural requirements for activity and act at ∼10 −6 mol m −3 ; these may act via specific receptors. In other cases, for example, the growth‐promoting action of other oligoxyloglucans, the stringency is somewhat lower and the activity is only seen at ∼10 −3 mol m −3 , and these oligosaccharins are proposed to influence growth through their ability to modulate the activity of an enzyme, cellulase. A third class of plant‐derived oligosaccharins, the oligo‐α‐galacturonides, appear to have much less stringent structural requirements; a number of unrelated physiological responses are evoked by the same range of oligo‐α‐galacturonides at ∼10 −3 to 1 mol m −3 . We suggest that these oligosaccharins may act via a mechanism not involving a specific receptor, perhaps by interacting with the plasma membrane to bring about a change in its physical properties.
Long noncoding RNA EWSAT1-mediated gene repression facilitates Ewing sarcoma oncogenesis (2014)

Chromosomal translocation that results in fusion of the genes encoding RNA-binding protein EWS and transcription factor FLI1 (EWS-FLI1) is pathognomonic for Ewing sarcoma. EWS-FLI1 alters gene expression through mechanisms that are not completely understood. We performed RNA sequencing (RNAseq) analysis on primary pediatric human mesenchymal progenitor cells (pMPCs) expressing EWS-FLI1 in order to identify gene targets of this oncoprotein. We determined that long noncoding RNA-277 (Ewing sarcoma-associated transcript 1 [EWSAT1]) is upregulated by EWS-FLI1 in pMPCs. Inhibition of EWSAT1 expression diminished the ability of Ewing sarcoma cell lines to proliferate and form colonies in soft agar, whereas EWSAT1 inhibition had no effect on other cell types tested. Expression of EWS-FLI1 and EWSAT1 repressed gene expression, and a substantial fraction of targets that were repressed by EWS-FLI1 were also repressed by EWSAT1. Analysis of RNAseq data from primary human Ewing sarcoma further supported a role for EWSAT1 in mediating gene repression. We identified heterogeneous nuclear ribonucleoprotein (HNRNPK) as an RNA-binding protein that interacts with EWSAT1 and found a marked overlap in HNRNPK-repressed genes and those repressed by EWS-FLI1 and EWSAT1, suggesting that HNRNPK participates in EWSAT1-mediated gene repression. Together, our data reveal that EWSAT1 is a downstream target of EWS-FLI1 that facilitates the development of Ewing sarcoma via the repression of target genes.
Airway tissue factor-dependent coagulation activity in response to sulfur mustard analog 2-chloroethyl ethyl sulfide (2011)

Acute lung injury is a principal cause of morbidity and mortality in response to mustard gas (SM) inhalation. Obstructive, fibrin-containing airway casts have recently been reported in a rat inhalation model employing the SM analog 2-chloroethyl ethyl sulfide (CEES). The present study was designed to identify the mechanism(s) causing activation of the coagulation cascade after CEES-induced airway injury. Here we report that CEES inhalation elevates tissue factor (TF) activity and numbers of detached epithelial cells present in lavage fluid (BALF) from rats after exposure (18 h). In vitro studies using 16HBE cells, or with rat BALF, indicated that detached epithelial cells could convert factor X (FX) to the active form FXa when incubated with factor VII and could elicit rapid clotting of plasma. In addition, immunocytochemical analysis demonstrated elevated cell surface (TF) expression on CEES-exposed 16HBE cells as a function of time. However, total cell TF expression did not increase. Since membrane surfaces bearing TF are important determinants of clot initiation, anticoagulants directed against these entities were tested for ability to limit plasma clotting or FX activation capacity of BALF or culture media. Addition of tifacogin, a TF pathway inhibitor, effectively blocked either activity, demonstrating that the procoagulant actions of CEES were TF pathway dependent. Lactadherin, a protein capable of competing with clotting factors for phospholipid-binding sites, was partially effective in limiting these procoagulant actions. These findings indicate that TF pathway inhibition could be an effective strategy to prevent airway obstruction after SM or CEES inhalation.
Cold Eyes and Warm Hearts: changing perspectives on teachers’ work and careers (1990)

Until fairly recently views of teacher careers were dominated by notions of an objective, progressive structure populated by males. Though the majority of teachers were women, they were not reckoned to have careers within the profession. They occupied the broad, flat base of the squat opportunity pyramid, together with some starter, and older 'failed' males. Furthermore, their residence there was intermittent, as they took time off for child-bearing and -rearing. Teaching was thus a useful occupation for women, as their role was perceived, as it allowed for such exits and re-entries.
PepAssem, a Peptide Assembly Algorithm for a Complex Protein Mixture (2001)

Analysis of a complex protein mixture is one of the main topics in proteomics. While the traditional 2D-PAGE method separates proteins first and then identifies each spot, an alternative method was proposed which cleavages the whole protein mixture with an enzyme like trypsin, separates the resulted peptide fragments with multi-dimensional LC, identifies each fragment with mass spectrometry, and then reconstructs the protein components via database searching [3, 6]. Although this new methodology is very promising, it cannot detect proteins that do not exist in databases or the corresponding DNA sequences are not known. We investigated a new shotgun approach using multiple cleavage schemes and a peptide assembly program. Ideal peptide fragments resulting from multiple digestions with several peptidases partially overlap each other and can be assembled as shotgun sequencing of DNA fragments. Starting from the non-redundant set of proteome in the SWISS-PROT database, we theoretically generated peptide fragments and used it as a test set. Our simulation showed that a part of the original complex protein mixture could be identified without any additional information other than short peptide sequences and masses of original proteins.
On the Precision Attainable with Various Floating-Point Number Systems (1973)

For scientific computations on a digital computer the set of real numbers is usually approximated by a finite set F of ``floating-point'' numbers. We compare the numerical accuracy possible with different choices of F having approximately the same range and requiring the same word length. In particular, we compare different choices of base (or radix) in the usual floating-point systems. The emphasis is on the choice of F, not on the details of the number representation or the arithmetic, but both rounded and truncated arithmetic are considered. Theoretical results are given, and some simulations of typical floating-point computations (forming sums, solving systems of linear equations, finding eigenvalues) are described. If the leading fraction bit of a normalized base-2 number is not stored explicitly (saving a bit), and the criterion is to minimize the mean square roundoff error, then base 2 is best. If unnormalized numbers are allowed, so the first bit must be stored explicitly, then base 4 (or sometimes base 8) is the best of the usual systems.
Adding Slow-Silent Virtual Channels for Low-Power On-Chip Networks (2008)

In this paper, we introduce the use of slow-silent virtual channels to reduce the switching power of on-chip networks while keeping the leakage power small. Adding virtual channels to a network improves the throughput until each link bandwidth is saturated. This enables us to reduce the switching power of on-chip networks by decreasing their operating frequency and supply voltage. However, adding virtual channels increases the leakage power of routers as well as the area due to their large buffers; so the runtime power gating is applied to individual virtual channels to eliminate this problem. We evaluate the performance of slow-silent virtual channels by using real application traces, and their power consumption (switching and leakage) is evaluated based on the detailed design of a virtual-channel router placed and routed with a 90 nm technology. These evaluation results show that a network with three or four virtual channels achieves the best energy efficiency in a uniform traffic. In the cases of neighboring communications, a network with two virtual channels is better than the other networks with more virtual channels, because the performance improvement from no virtual channel to two virtual channels is the largest and their frequency and supply voltage can also be reduced well in these cases.
Location-Transparent Integration of Distributed OSGi Frameworks and Web Services (2009)

The OSGi Alliance defines an open, modular, and scalable service delivery platform. The DPWS specification standardizes the process of consuming and exposing Web Services in a lightweight footprint. In our work, we provide a solution for the mutual integration of OSGi and DPWS. The approach adopts the mechanisms of distributed object systems. It employs OSGi-based service proxies and service skeletons.
CD123 AML targeting by chimeric antigen receptors (2014)

Chimeric antigen receptor (CAR) modified T cells have emerged as powerful tools for controlling leukemias. We recently showed that anti-CD123 CAR-expressing cytokine-induced killer T cell treatment is an effective immunotherapeutic approach to eradicate Acute Myeloid Leukemia (AML) cells. Here, we discuss how this genetically modified cell-based strategy could be relevant to the field of AML therapeutics.
Diurnal Variation of Plasma Minerals and Trace Elements in a Group of Japanese Male Adults. (1993)

When the nutritional status of minerals and essential trace elements is assessed by their levels in plasma, intra-individual variation in the measured values, particularly diurnal variation, must be considered. In this paper, concentrations of nine elements (Na, Mg, P, K, Ca, Fe, Cu, Zn, and Se) in plasma collected 7 times in a 24-h period from 10 healthy Japanese adults were measured with hemoglobin and plasma concentrations of proteins (total protein, albumin, retinol binding protein (RBP), ceruloplasmin, and transferrin), total cholesterol, and cortisol. Then the pattern of diurnal variation in, and the interrelationships among, these parameters were clarified in subjects who consumed an ordinary meal. Significant diurnal variation examined by two-way analysis of variance (variations due to subject and sampling time) was found for Zn, RBP, and cortisol. Plasma Zn level was higher in the forenoon samples than in those taken in the afternoon. The pattern of diurnal variation found for cortisol was similar to, but slightly different from, that for Zn. The correlation coefficient between Zn and cortisol was significant (r = 0.555) using the standardized values for individuals, and in the result of multiple regression analysis, cortisol level was selected as the significant explanatory factor for Zn variation; however, the sampling time was the most significant factor. For other elements besides Zn, no significant diurnal variation was found. Moreover, no meaningful factors were selected for variations of those elements in the result of multiple regression analysis. These results indicate that, in assessing Zn nutriture with its plasma levels, it is necessary to unify the sampling time.
One-Step Construction of the Pentacyclic Skeleton of Saframycin A from a “Trimer” of α-Amino Aldehydes (2000)

The entire skeleton of the saframycin antitumor antibiotics is assembled in one remarkable transformation (8 --> 9) from an N-linked oligomer of three alpha-amino aldehyde components, a reaction pathway that may parallel the biosynthetic route to the saframycins.
Reactive Oxygen-Mediated Protein Oxidation in Aging and Disease (1997)

ADVERTISEMENT RETURN TO ISSUEPREVOpen ForumNEXTReactive Oxygen-Mediated Protein Oxidation in Aging and DiseaseEarl R. Stadtman and Barbara S. BerlettView Author Information Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Building 3, Room 222, Bethesda, Maryland 20892-0342 Cite this: Chem. Res. Toxicol. 1997, 10, 5, 485–494Publication Date (Web):May 19, 1997Publication History Received30 July 1996Published online19 May 1997Published inissue 1 May 1997https://doi.org/10.1021/tx960133rCopyright © 1997 American Chemical SocietyRIGHTS & PERMISSIONSArticle Views2385Altmetric-Citations541LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit Read OnlinePDF (291 KB) Get e-AlertscloseSUBJECTS:Carbonyls,Modification,Monomers,Oxidation,Peptides and proteins Get e-Alerts
Ultrafast Digital Printing toward 4D Shape Changing Materials (2016)

Ultrafast 4D printing (<30 s) of responsive polymers is reported. Visible-light-triggered polymerization of commercial monomers defines digitally stress distribution in a 2D polymer film. Releasing the stress after the printing converts the structure into 3D. An additional dimension can be incorporated by choosing the printing precursors. The process overcomes the speed limiting steps of typical 3D (4D) printing.
The Isoforms of the p53 Protein (2009)

p53 is a transcription factor with a key role in the maintenance of genetic stability and therefore preventing cancer formation. It belongs to a family of genes composed of p53, p63, and p73. The p63 and p73 genes have a dual gene structure with an internal promoter in intron-3 and together with alternative splicing, can express 6 and 29 mRNA variants, respectively. Such a complex expression pattern had not been previously described for the p53 gene, which was not consistent with our understanding of the evolution of the p53 gene family. Consequently, we revisited the human p53 gene structure and established that it encodes nine different p53 protein isoforms because of alternative splicing, alternative promoter usage, and alternative initiation sites of translation. Therefore, the human p53 gene family (p53, p63, and p73) has a dual gene structure. We determined that the dual gene structure is conserved in Drosophila and in zebrafish p53 genes. The conservation through evolution of the dual gene structure suggests that the p53 isoforms play an important role in p53 tumor-suppressor activity. We and others have established that the p53 isoforms can regulate cell-fate outcome in response to stress, by modulating p53 transcriptional activity in a promoter and stress-dependent manner. We have also shown that the p53 isoforms are abnormally expressed in several types of human cancers, suggesting that they play an important role in cancer formation. The determination of p53 isoforms' expression may help to link clinical outcome to p53 status and to improve cancer patient treatment.
Thermal Conductivity, Heat Capacity, and Elastic Constants of Water-Soluble Polymers and Polymer Blends (2016)

We use time-domain thermoreflectance (TDTR), and the generation and detection of longitudinal and surface acoustic waves, to study the thermal conductivity, heat capacity, and elastic properties of thin films of poly(vinyl alcohol) (PVA), poly(acrylic acid) (PAA), polyacrylamide (PAM), poly(vinylpyrrolidone) (PVP), methyl cellulose (MC), poly(4-styrenesulfonic acid) (PSS), poly(N-acryloylpiperidine) (PAP), poly(methyl methacrylate) (PMMA), and a polymer blend of PVA/PAA. The thermal conductivity of six water-soluble polymers in the dry state varies by a factor of ≈2, from 0.21 to 0.38 W m–1 K–1, where the largest values appear among polymers with a high concentration of hydrogen bonding (PAA, PAM, PSS). The longitudinal elastic constants range from 7.4 to 24.5 GPa and scale linearly with the shear elastic constants, suggesting a narrow distribution of Possion’s ratio 0.35 < ν < 0.40. The thermal conductivity increases with the average sound velocity, as expected based on the model of the minimum thermal conductivity. The thermal conductivity of polymer blends of PVA (0.31 W m–1 K–1) and PAA (0.37 W m–1 K–1) is in agreement with a simple rule of mixtures.
Disarming <i>Staphylococcus aureus</i> from destroying human cells by simultaneously neutralizing six cytotoxins with two human monoclonal antibodies (2017)

Pathogenesis of Staphylococcus aureus is increasingly recognized to be driven by powerful toxins. Staphylococcus aureus employs up to six pore-forming toxins to subvert the human host defense and to promote bacterial invasion: alpha-hemolysin that disrupts epithelial and endothelial barriers and five leukocidins that lyse phagocytes involved in bacterial clearance. Previously, we described two human monoclonal antibodies (mAbs), ASN-1 that neutralizes alpha-hemolysin and four leukocidins (LukSF-PV, LukED, HlgAB, HlgCB), and ASN-2 that inactivates the 5<sup>th</sup> leukocidin, LukGH. In this study we tested the individual and combined effects of ASN-1 and ASN-2 in multiple in vitro models employing relevant human target cells. We found that diverse S. aureus isolates with different genetic backgrounds (based on MLST- and spa-typing) and antibiotic sensitivity (both MRSA and MSSA) displayed greatly different cytotoxin expression patterns influenced by the type of growth medium used. Both mAbs were required to fully prevent the lysis of human neutrophils exposed to the mixture of recombinant cytotoxins or native toxins present in the culture supernatants of S. aureus isolates. Flow cytometry confirmed the protective effects of ASN-1 + ASN-2 (known as ASN100) on granulocytes, monocytes, NK-cells and T-lymphocytes. ASN-1 alone preserved the integrity of a 3D-primary culture of human tracheal/bronchial mucociliary epithelial tissue infected with S. aureus. We conclude that simultaneous inhibition of alpha-hemolysin and five leukocidins by ASN100 blocks cytolytic activity of S. aureus towards human target cells in vitro.
Activated factor X and thrombin formation triggered by tissue factor on endothelial cell matrix in a flow model: effect of the tissue factor pathway inhibitor (1994)

The procoagulant subcellular matrix of stimulated endothelial cells that contains tissue factor (TF) was used to investigate the mechanism by which TF pathway inhibitor (TFPI) inhibits thrombin formation initiated by TF/factor VIIa (FVIIa) under flow conditions. Purified coagulation factors VII, X, and V and prothrombin were perfused at a wall shear rate of 100 s-1 through a flow chamber containing a coverslip covered with matrix of cultured human umbilical vein endothelial cells. This resulted in a TF- and FVII-dependent FXa and thrombin generation as measured in the effluent at the outlet of the system. Inhibition of this TF/FVIIa-triggered thrombin formation by TFPI purified from plasma was dependent on the amount of TF present on the endothelial cell matrix. The rate of prothrombinase assembly and steady-state levels of thrombin formation were decreased by TFPI. Because persistent albeit decreased steady-state levels of thrombin formation occurred in the presence of TFPI, we conclude that plasma-TFPI does not inhibit FXa present in the prothrombinase complex. The addition of FIX and FVIII to perfusates containing FVII and FX increased the FXa generation on endothelial matrices, and counteracted the inhibition of thrombin formation on endothelial cell matrices by TFPI. Our data provide further evidence for the hypothesis that the rapid inactivation of TF/FVIIa by TFPI in combination with the absence of either FVIII or FIX causes the bleeding tendency of patients with hemophilia A or B.
Stretchable electronics based on Ag-PDMS composites (2014)

Patterned structures of flexible, stretchable, electrically conductive materials on soft substrates could lead to novel electronic devices with unique mechanical properties allowing them to bend, fold, stretch or conform to their environment. For the last decade, research on improving the stretchability of circuits on elastomeric substrates has made significant progresses but designing printed circuit assemblies on elastomers remains challenging. Here we present a simple, cost-effective, cleanroom-free process to produce large scale soft electronic hardware where standard surface-mounted electrical components were directly bonded onto all-elastomeric printed circuit boards, or soft PCBs. Ag-PDMS tracks were stencil printed onto a PDMS substrate and soft PCBs were made by bonding the top and bottom layers together and filling punched holes with Ag-PDMS to create vias. Silver epoxy was used to bond commercial electrical components and no mechanical failure was observed after hundreds of stretching cycles. We also demonstrate the fabrication of a stretchable clock generator.
Speech Training Helps Preaching (1954)

My college training in speech has been in valuable to me in my profession as a minister. 1 can best summarize its help as follows.
Changes in estrogen receptor, progesterone receptor and Her-2/neu status with time: discordance rates between primary and metastatic breast cancer. (2009)

This series suggests that significant discordance exists for hormone receptor status between primary and metastatic breast cancer samples. Loss of PR was particularly frequent.
Behavioral Side Effects in Obsessive-Compulsive Patients Treated With Fluvoxamine (1994)

Diaferia, Griseppina MD; Mundo, Emanuela MD; Bianchi, Yvonne MD; Ronchi, Paolo MD Author Information
GOLYTELY: A NEW APPROACH TO FAECAL IMPACTION IN OLD AGE (1986)

Forty-five elderly subjects with faecal impaction were entered into a study of the efficacy of golytely. Twenty-three were treated with golytely 2 l/day for 2 days and then lactulose 30 ml b.d. and daily enemas. Twenty-two were treated with lactulose 30 ml b.d. and daily enemas alone. A good response was achieved in 13 (56%) within 1 week and in 20 (87%) within 2 weeks when golytely was used, as compared to only 5 (23%) within 1 week and 9 (41%) within 2 weeks when it was not used.
Contrast-enhanced synthetic MRI for the detection of brain metastases (2016)

Synthetic T1IR imaging created better contrast compared with synthetic T1W or conventional T1IR imaging. The ability to detect brain metastases was comparable among these imaging.