gebhart/patrepeval huggingface dataset:
Expression profiling reveals multiple myelin alterations in murine succinate semialdehyde dehydrogenase deficiency (2006)

Succinic semialdehyde dehydrogenase (SSADH) deficiency, a rare genetic defect of GABA degradation recently modelled in mice (SSADH(-/-) mice), manifests early absence seizures that evolve into generalized convulsive seizures and lethal status epilepticus in gene-ablated mice. Disrupted GABA homeostasis, in conjunction with the epileptic phenotype and increased gamma-hydroxybutyric acid (GHB), suggested that expression profiling with the U74Av2 Affymetrix system would reveal dysregulation of receptor genes associated with GABAergic and glutamatergic neurotransmission. Unexpectedly, we found significant downregulation for genes associated with myelin biogenesis and compaction, predominantly in hippocampus and cortex. These results were confirmed by: (1) myelin basic protein (MBP) immunohistochemistry; (2) western blotting of myelin-associated glycoprotein (MAG) and MBP; (3) qRT-PCR analyses of myelin-associated oligodendrocytic basic protein (MOBP), MAG, MBP and proteolipid protein (PLP) in hippocampus, cortex and spinal cord; (4) quantitation of ethanolamine and choline plasmalogens, all core myelin components; (5) evaluation of myelin content in brain sections employing toluidine blue staining; and (6) ultrastructural evaluation of myelin sheath thickness via electron microscopy. We speculate that increased GABA/GHB, acting through GABAergic systems, results in decreased levels of the neurosteroids progesterone and allopregnanolone [Gupta et al (2003) Ann Neurol 54(Supplement 6): S81-S90] and phosphorylation of mitogen-activated protein (MAP) kinase, with resulting myelin protein abnormalities primarily in the cortex of SSADH(-/-) mice.
Local area network characteristics, with implications for broadband network congestion management (1991)

The authors examine the phenomenon of congestion to better understand the congestion management techniques that will be needed in high-speed, cell-based networks. The first step of this study is to use high time-resolution local area network (LAN) traffic data to explore the nature of LAN traffic variability. Then the data are used for a trace-driven simulation of a connectionless service that provides LAN interconnection. The simulation allows one to characterize what congestion might look like in a high-speed, cell-based network. When realistic data are applied to simple models of LAN interconnection, it is observed that during periods, congestion persists and losses can be significant; congestion losses cannot be avoided by modest increases in buffer capacity; consequences of misengineering can be serious; and, fortunately, most congested periods are preceded by signs of impending danger.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Effect of solutes on the cold-induced insolubility of monoclonal cryoimmunoglobulins. (1977)

The effects of a variety of compounds upon the cold-induced insolubility of the IgM-K cryoglobulin McE have been examined. Cryoprecipitation was found to be inhibited by certain neutral salts, ureas, amides, tetraalkylammonium salts, long chain sodium alkyl sulfates, sugars, and a number of other agents. Cryoprecipitation was enhanced by increasing the hydrophobicity of alcohols, ureas, amides, and tetraalkylammonium salts, as well as by low concentrations of many solutes. With the exception of the alkyl sulfates, inhibition was not accompanied by detectable changes in conformation of the cryoimmunoglobulin. These inhibitions and enhancements were also associated with changes in the temperature at which cryoprecipitation was initiated as well as the temperature at which a low temperature-induced conformation change occurs in the McE protein. The effects of solutes on McE are compared to results obtained with five other (two IgG, three IgM) cryoimmunoglobulins, and it is hypothesized that electrostatic and dispersion forces are primarily responsible for the cold insolubility of monoclonal cryoimmunoglobulins.
Algorithm for solving the Welch-Berlekamp key-equation, with a simplified proof (1993)

An alternative technique due to Welch and Berlekamp for decoding Reed-Solomon codes has a key equation different in form from the key equation solved by the conventional Berlekamp-Massey algorithm or by the so-called Euclidean algorithm. The Letter presents an algorithm for solving the key equation which has a simple structure and which is readily shown to work.
Platelet Activation in Acute Myocardial Infarction and Unstable Angina Is Inhibited by Nitric Oxide Donors (1996)

Platelet activation and thrombus formation within the coronary artery are major factors in acute myocardial infarction (AMI) and unstable angina (UA), and continuing platelet activation is associated with an adverse prognosis. We assessed platelet activation by using flow cytometry to measure platelet surface expression of P-selectin and glycoprotein IIb/IIIa in 20 patients with AMI and 20 with UA, all of whom were treated with aspirin. Platelet studies were repeated after the infusion of a nitric oxide donor (glyceryl trinitrate or S-nitrosoglutathione) that produced a fall in mean arterial pressure of no more than 10 mm Hg. P-selectin was expressed on 2.5% (range, 1.4% to 6.3%) of platelets from AMI and 2.3% (range, 1.6% to 3.3%) from UA subjects compared with 1.0% (range, 0.6% to 1.9%) of platelets from 20 control volunteers without angina (P < .001). Glycoprotein IIb/IIIa expression was 101.6 +/- 2.7 arbitrary units of relative fluorescence in AMI and 100.2 +/- 3.3 in UA compared with 87.8 +/- 2.5 in control subjects (P < .01). In both AMI and UA, S-nitrosoglutathione reduced P-selectin (P < .001) and glycoprotein IIb/IIIa (P < .05) expression, as did glyceryl trinitrate (P < .02 and P < .01, respectively). In 3 of 20 patients receiving glyceryl trinitrate the lowest dose was not tolerated due to headache or hypotension. These findings show that platelet activation persists in AMI and UA despite aspirin treatment and that this can be inhibited by using glyceryl trinitrate or S-nitrosoglutathione. S-nitrosoglutathione is better tolerated at the doses required.
Alkylcopper(I) in the coupling of Grignard reagents with alkyl halides (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlkylcopper(I) in the coupling of Grignard reagents with alkyl halidesJay K. Kochi and Masuhiko TamuraCite this: J. Am. Chem. Soc. 1971, 93, 6, 1485–1487Publication Date (Print):March 1, 1971Publication History Published online1 May 2002Published inissue 1 March 1971https://pubs.acs.org/doi/10.1021/ja00735a029https://doi.org/10.1021/ja00735a029research-articleACS PublicationsRequest reuse permissionsArticle Views1566Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
FT-SERS Studies on Molecular Recognition Capabilities of Monolayers of Novel Nucleolipid Amphiphiles (2000)

The molecular recognition effect in nucleic acids was simulated in the monolayers formed by six novel nucleolipid amphiphiles. Fourier transform surface-enhanced Raman scattering (FT-SERS) technique was introduced into the research area of molecular recognition occurring in an interface system. High-quality FT-SERS spectra of a single Langmuir−Blodgett (LB) monolayer of the nucleolipid amphiphiles were obtained. Characteristic vibrational modes of the corresponding complementary nucleic acid bases, which transferred along with the monolayers of nucleolipid amphiphiles into the LB films, were clearly seen. The mechanism of molecular recognition through multiple hydrogen bonds between complementary bases was described. It was proved that this technique can be used as a powerful tool for studying molecular recognition in interface systems because of its high sensitivity.
A system for efficient dissemination of weather forecasts for sustainable solar-powered sensors (2015)

Depletion-safe and efficient operation of sensor networks powered by miniature solar harvesters demands precise prediction of the future energy intake. Only recently, methods that join local knowledge and global weather forecasts have been shown to improve the prediction performance. However, making use of global weather forecasts requires network-wide dissemination with low energy and resource consumption. We present and evaluate a system architecture that automatically obtains global weather forecasts from freely available online resources and disseminates them into the sensor network. We implemented our system for sensor network hardware based on TinyOS and Java. We conducted a system integration test to verify the functionality of all components and evaluated the performance of the dissemination algorithm with testbed experiments. While we find that energy consumption is negligible and resource usage is low, dissemination was reliable with a low delay compared to the hourly interval of forecast updates.
Restoration of noisy images blurred by a random medium (2003)

An image restoration model is given to restore images blurred by a random medium. The overall distortion process caused by the random medium is represented by a stochastic point spread function. To estimate the original images degraded by this model, two restoration algorithms are formulated based on the Wiener filter and the constrained least-squares filter. Computer simulations are included to compare the performances of the proposed algorithms with the conventional ones.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
7-Nitroindazole, but Not Aminoguanidine, Attenuates the Acute Inflammatory Responses and Brain Injury during the Early Phase of <i>Escherichia coli</i> Meningitis in the Newborn Piglet (2001)

We evaluated the anti-inflammatory and neuroprotective effects of the selective neuronal nitric oxide synthase inhibitor 7-nitroindazole and aminoguanidine, which predominantly inhibits inducible nitric oxide synthase, during the early phase of experimental bacterial meningitis in the newborn piglet. Meningitis was induced by intracisternal injection of 10(8) colony-forming units of Escherichia coli in 100 microl of saline. 7-Nitroindazole significantly attenuated the meningitis-induced acute inflammatory responses such as increased intracranial pressure, decreased cerebrospinal fluid (CSF) glucose concentration, and CSF leukocytosis at 2 h. However, meningitis-induced CSF leukocytosis at 4 h and increased CSF lactate and tumor necrosis factor alpha levels were not significantly attenuated. Reduced cerebral cortical cell membrane Na(+),K(+)-ATPase activity and increased lipid peroxidation products, indicative of meningitis-induced brain cell membrane dysfunction, were also significantly improved with 7-nitroindazole treatment. In contrast, although aminoguanidine significantly attenuated the increase in the CSF tumor necrosis factor alpha level, it failed to attenuate the acute inflammation and the ensuing brain injury in bacterial meningitis. In summary, 7-nitroindazole, but not aminoguanidine, significantly attenuated the acute inflammatory responses and brain injury during the early phase of neonatal bacterial meningitis.
GemSIM: general, error-model based simulator of next-generation sequencing data (2012)

Next-generation sequencing has unprecedented potential for assessing genetic diversity, however analysis is complicated as error profiles can vary noticeably even between different runs of the same technology. Simulation with GemSIM can help overcome this problem, by providing insights into the error profiles of individual sequencing runs and allowing researchers to assess the effects of these errors on downstream data analysis.
Tina: Its achievements and its future directions (2000)

The telecommunications information networking architecture (TINA) has reached a mature state and TINA solutions are now appearing in the market. This article presents a brief sketch of TINA's past and the overall objectives as they are seen today. It further describes the current technological state of the architecture with its business model, the computing environment requirements, the session model, the networking resource model, and its impact on the network technology evolution. Eventually, the path TINA will take into the future is indicated by outlining the TINA Trial project and the workgroup activities; the driving forces during TINA's current phase.
Genetic Variation for Lettuce Seed Thermoinhibition Is Associated with Temperature-Sensitive Expression of Abscisic Acid, Gibberellin, and Ethylene Biosynthesis, Metabolism, and Response Genes     (2008)

Lettuce (Lactuca sativa 'Salinas') seeds fail to germinate when imbibed at temperatures above 25 degrees C to 30 degrees C (termed thermoinhibition). However, seeds of an accession of Lactuca serriola (UC96US23) do not exhibit thermoinhibition up to 37 degrees C in the light. Comparative genetics, physiology, and gene expression were analyzed in these genotypes to determine the mechanisms governing the regulation of seed germination by temperature. Germination of the two genotypes was differentially sensitive to abscisic acid (ABA) and gibberellin (GA) at elevated temperatures. Quantitative trait loci associated with these phenotypes colocated with a major quantitative trait locus (Htg6.1) from UC96US23 conferring germination thermotolerance. ABA contents were elevated in Salinas seeds that exhibited thermoinhibition, consistent with the ability of fluridone (an ABA biosynthesis inhibitor) to improve germination at high temperatures. Expression of many genes involved in ABA, GA, and ethylene biosynthesis, metabolism, and response was differentially affected by high temperature and light in the two genotypes. In general, ABA-related genes were more highly expressed when germination was inhibited, and GA- and ethylene-related genes were more highly expressed when germination was permitted. In particular, LsNCED4, a gene encoding an enzyme in the ABA biosynthetic pathway, was up-regulated by high temperature only in Salinas seeds and also colocated with Htg6.1. The temperature sensitivity of expression of LsNCED4 may determine the upper temperature limit for lettuce seed germination and may indirectly influence other regulatory pathways via interconnected effects of increased ABA biosynthesis.
SS18 Together with Animal-Specific Factors Defines Human BAF-Type SWI/SNF Complexes (2012)

SWI/SNF complexes are found in most eukaryotes and in the course of evolution new SWI/SNF subunits appeared. SS18 is found in plants as well as animals. Our results suggest that in both protostome and deuterostome animals, a class of BAF-type SWI/SNF complexes will be found that harbor SS18 or its paralogs, along with ARID1, DPF and BCL7 paralogs. Those BAF complexes are proteomically distinct from the eukaryote-wide PBAF-type SWI/SNF complexes. Finally, our results suggests that the human bromodomain factors BRD7 and BRD9 associate with PBAF and BAF, respectively.
Separation and Characterization of Vanadyl Porphyrins in Venezuela Orinoco Heavy Crude Oil (2013)

Venezuela Orinoco heavy crude oil was sequentially separated into several subfractions to determine the contents and types of vanadyl porphyrins contained in the products. Vanadium contents in each subfraction were detected using an atomic absorption spectrometer (AAS) combined with the characterization of vanadyl porphyrins by ultraviolet–visible (UV–vis) spectroscopy and positive-ion electrospray ionization (ESI) Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). Six types of petroleum vanadyl porphyrins, which have been identified previously, were well-characterized with the detailed fractionation. Three new series of vanadyl porphyrins corresponding to molecules of CnHmN4VO2, CnHmN4VO3, and CnHmN4VO4, respectively, were identified in addition to the six known types of vanadyl porphyrins.
A mechanism for secretory IgA-mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. (1979)

The ability of Eimeria tenella sporozoites to develop normally in cultured chick kidney cells was used as an indicator of the anticoccidial effects of sera and extracts of caecal contents or tissue. Pre-treatment of sporozoites with normal serum globulin enhanced the frequency of intracellular development but pre-treatment in balanced salt solution, without protein, damaged sporozoites so that most had lost the ability to differentiate, even when they were able to invade host cells. The same inhibitory effect was seen when sporozoites were incubated in extracts of caecal contents from non-immunized chickens, although parasitic development was unaffected when sporozoites were pretreated in similar extracts of mucosae. Extracts of immune caecal contents impaired both cell penetration and subsequent development. These results show that sporozoites can lose the ability to differentiate before the ability to penetrate cells and provide evidence of a possible synergism between non-specific factors and secretory antibodies in anticoccidial immunity.
Demonstration of the functional importance of the Gal epitope in an ex vivo model of xenotransplantation (1997)

Abstract: The galactose a 1‐3 galactose terminal disaccharide (Gal epitope) has been identified as the major porcine xenoantigen recognised by xenoantibody in human plasma. Elimination or suppression of the epitope or antibody will be a major factor in overcoming hyperacute rejection. Inhibition of the antibody by depletion or elimination of the epitope by gene knockout may reveal the importance of other xenoantibodies, and in addition elimination of the epitope may unmask or produce other xenoantibody combinations. This study aims to determine the relative importance of anti‐Gal antibody and Gal epitope elimination in a functional model of xenotransplantation, ex vivo perfusion of mouse hearts with human plasma on a Langendorff apparatus. Perfusion of mouse hearts with human plasma depleted of anti‐Gal antibody demonstrates a protective effect compared to hearts perfused with undepleted plasma with prolongation of survival time from 24.1 to 44.5 min. Similarly, elimination of the epitope is also protective. Hearts from Gal knockout mice, which were generated by gene targeting of the al,3 galactosyltransferase gene, and hearts from appropriate control mice were perfused with human plasma. Gal knockout mice hearts demonstrated an increase in survival time from 10.2 to 33.8 min compared to control hearts. This was accompanied by a decrease in C3c and IgM, but little change in IgG deposition. The protective effect is incomplete, probably due to the effect of antibodies against non‐Gal xenoantigens. There was no functional evidence for generation of neo‐antigens in the Gal KO mice that were I recognised by naturally occurring human xenoantibodies.
Evaluation of delta-aminolaevulinic acid in blood of workers exposed to lead. (1993)

Exposure-effect and exposure-response relation between exposure to lead and delta-aminolaevulinic acid concentration in blood (ALA-B) were examined in 238 male workers exposed to lead. Concentrations of ALA-B ranged from 26 to 352 micrograms/l and lead concentrations in blood (Pb-B) from 7.1 to 86.0 micrograms/dl. Concentrations of ALA-B correlated closely with concentrations of Pb-B (r = 0.74), and increased ALA-B concentration occurred at Pb-B concentrations of around 30 micrograms/dl. Exposure-response curves indicated that the 50 percentile response doses were roughly 30, 40, and 50 micrograms/dl Pb-B when cut off points of ALA-B were set at 50, 60, and 70 micrograms/l respectively. The sensitivity and specificity of measurements of ALA-B concentrations for health screening were sufficiently high when the health based exposure limits of lead were set at 30-50 micrograms/dl. Moreover, a pronounced increase in ALA-B concentrations occurred when the inhibition rate of erythrocyte ALA dehydratase exceeded 85%. These findings suggest that ALA-B is a useful indicator for assessing the early effects of exposure to lead on haem biosynthesis.
Alkaloide aus <i>Cynanchum vincetoxicum</i> (L.) Pers., II<sup>1)</sup> Synthese des (±)‐6‐Hydroxy‐2.3‐dimethoxy‐9.11.12.13.13a.14‐hexahydro‐dibenzo[<i>f</i>,<i>h</i>]pyrrolo[1.2‐<i>b</i>]isochinolins (1970)

Abstract Die Synthese von 3 wird beschrieben. Dessen UV‐, IR‐ und Massenspektren sind identisch mit denen des natürlich vorkommenden, linksdrehenden Alkaloids. (±)‐ 3 wird zu (±)‐ 2 methyliert, das mit dem aus optisch aktivem 2 gewonnenen Racemat in allen analytischen Kriterien übereinstimmt.
Reduced Infectivity in Cattle for an Outer Membrane Protein Mutant of Anaplasma marginale (2015)

Anaplasma marginale is the causative agent of anaplasmosis in cattle. Transposon mutagenesis of this pathogen using the Himar1 system resulted in the isolation of an omp10 operon insertional mutant referred to as the omp10::himar1 mutant. The work presented here evaluated if this mutant had morphological and/or growth rate defects compared to wild-type A. marginale. Results showed that the morphology, developmental cycle, and growth in tick and mammalian cell cultures are similar for the mutant and the wild type. Tick transmission experiments established that tick infection levels with the mutant were similar to those with wild-type A. marginale and that infected ticks successfully infected cattle. However, this mutant exhibited reduced infectivity and growth in cattle. The possibility of transforming A. marginale by transposon mutagenesis coupled with in vitro and in vivo assessment of altered phenotypes can aid in the identification of genes associated with virulence. The isolation of deliberately attenuated organisms that can be evaluated in their natural biological system is an important advance for the rational design of vaccines against this species.
Effect of Rhamnolipids on Growth of Pseudomonas aeruginosa Mutant Deficient in n-Paraffin-utilizing Ability (1972)

Journal Article Effect of Rhamnolipids on Growth of Pseudomonas aeruginosa Mutant Deficient in n-Paraffin-utilizing Ability Get access Seiga Itoh, Seiga Itoh Tokyo Research Laboratory, Kyowa Hakko Kogyo, Co., Ltd., Machida-shi, Tokyo, Japan Search for other works by this author on: Oxford Academic Google Scholar Takeo Suzuki Takeo Suzuki Tokyo Research Laboratory, Kyowa Hakko Kogyo, Co., Ltd., Machida-shi, Tokyo, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 36, Issue 12, 1 December 1972, Pages 2233–2235, https://doi.org/10.1080/00021369.1972.10860546 Published: 01 December 1972 Article history Received: 08 March 1972 Published: 01 December 1972