gebhart/patrepeval huggingface dataset:
Mapping and identification of interferon gamma-regulated HeLa cell proteins separated by immobilized pH gradient two-dimensional gel electrophoresis (1999)

Interferon gamma (IFN-gamma) is a potent immunomodulatory lymphokine, secreted by activated T-lymphocytes and NK-cells during the cellular immune response. Actions of IFN-gamma are mediated through binding to the IFN-gamma-receptor, present on most cells, and the subsequent activation of a great magnitude of IFN-gamma responsive genes has been reported previously. Our goal is to identify and map IFN-gamma-regulated HeLa cell proteins to the two-dimensional polyacrylamide gel electrophoresis with the immobilized pH gradient (IPG) two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) system. A semiconfluent layer of HeLa cells was grown on tissue culture plates, and changes in protein expression due to 100 U/mL IFN-gamma were investigated at different periods after treatment, using pulse labeling with [35S]methionine/cysteine in combination with 2-D PAGE (IPG). The identity of eight protein spots was elucidated by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), and several variants of the IFN-gamma-inducible tryptophanyl-tRNA synthetase (hWRS) were detected by immunoblotting.
The interactive online SKY/M‐FISH & CGH Database and the Entrez Cancer Chromosomes search database: Linkage of chromosomal aberrations with the genome sequence (2005)

To catalog data on chromosomal aberrations in cancer derived from emerging molecular cytogenetic techniques and to integrate these data with genome maps, we have established two resources, the NCI and NCBI SKY/M-FISH & CGH Database and the Cancer Chromosomes database. The goal of the former is to allow investigators to submit and analyze clinical and research cytogenetic data. It contains a karyotype parser tool, which automatically converts the ISCN short-form karyotype into an internal representation displayed in detailed form and as a colored ideogram with band overlay, and also has a tool to compare CGH profiles from multiple cases. The Cancer Chromosomes database integrates the SKY/M-FISH & CGH Database with the Mitelman Database of Chromosome Aberrations in Cancer and the Recurrent Chromosome Aberrations in Cancer database. These three datasets can now be searched seamlessly by use of the Entrez search and retrieval system for chromosome aberrations, clinical data, and reference citations. Common diagnoses, anatomic sites, chromosome breakpoints, junctions, numerical and structural abnormalities, and bands gained and lost among selected cases can be compared by use of the "similarity" report. Because the model used for CGH data is a subset of the karyotype data, it is now possible to examine the similarities between CGH results and karyotypes directly. All chromosomal bands are directly linked to the Entrez Map Viewer database, providing integration of cytogenetic data with the sequence assembly. These resources, developed as a part of the Cancer Chromosome Aberration Project (CCAP) initiative, aid the search for new cancer-associated genes and foster insights into the causes and consequences of genetic alterations in cancer.
Improved techniques for rotor fault detection in three-phase induction motors (2002)

A technique has been developed whereby faults in 3 phase induction motors can be detected by an analysis of a single, no-load, starting transient. The system has been developed on laboratory test-rigs and validated via computer simulation and industrial testing. The basis of the technique is explained and simulation and test data presented. Possible areas of future application and development are discussed.
The Laser Gingivectomy: The Use of the CO<sub>2</sub> Laser for the Removal of Phenytoin Hyperplasia (1985)

A new technique for the removal of phenytoin hyperplasia using the carbon dioxide (CO2) surgical laser is presented as well as a review of laser physics and the current uses of the CO2 laser in dentistry. Twelve cases of phenytoin hyperplasia removed surgically by the CO2 laser are presented; hence a laser gingivectomy. Advantages of the procedure include lack of hemorrhage yielding a dry field, noncontact surgery, sterilization of the surgical area, prompt healing, minimal post operative discomfort, and minimal time spent to perform the procedure. At the present time the use of the laser in dentistry is new and limited. In the future the laser may offer an alternative or an advancement to current procedures now used in dentistry.
Reduction of 1-Substituted 3-Oxidopyridiniums (1981)

v e 1-substituted 3-hydroxypiperidines b u t much
Laser glass composition and the possibility of eliminating electrostrictive effects (1971)

Many authors have stated that electrostrictive self-trapping of the light beam initiates laser damage in solid transparent dielectrics. By systematically varying composition, it may be possible to find a glass in which electrostrictive effects are eliminated.
Measurement of Interleukin‐21 (2007)

This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8(+) T cells using a flow cytometry-based analysis is described.
57.4L: <i>Late‐News Paper</i> : <i>Intelligent Backlight</i> : A Controllable Illumination System for High Efficiency and Sunlight Readable Mobile Displays (2014)

Abstract A novel directional backlight system for transmissive LCD illumination is described for the first time. By controlling the angular light distribution from the display, very low power consumption can be achieved for a required display luminance, or the backlight can operate with a conventional luminance distribution. Additionally very high display luminance can be achieved at the same power consumption levels as conventional backlight designs. Such a display can achieve good readability in high illuminance conditions, for example in direct sunlight. The backlight optical stack comprises a stepped waveguide Directional Light Guide Plate (D‐LGP), a micro‐structured reflective film and a polarisation recirculation structure that cooperates with an LED array to direct viewing windows to an observer with high coupling efficiency. The system implementation is described and measured performance from demonstrator displays is presented.
Question Similarity Calculation for FAQ Answering (2007)

Frequently asked question (FAQ) answering is a very useful module in automatic question answering (QA) systems where calculation of question similarity is a key problem. In this paper, we propose a new method for measuring the similarity between users' questions and the questions in a FAQ database. Both statistic measure and semantic information are employed. Statistic similarity is calculated based on dynamically formed vectors while semantic similarity is calculated by utilizing word similarity based on WordNet. Overall similarity is a combination of statistic similarity and semantic similarity. Preliminary results show that our method achieves a good performance.
Nucleotide sequence of the Korean strain of hop stunt viroid (HSV) (1988)

Journal Article Nucleotide sequence of the Korean strain of hop stunt viroid (HSV) Get access Jai Youl Lee, Jai Youl Lee Max-Planck-Institut für Biochemic, Abteilung ViroidforschungD-8033 Martinsried bei München, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Holger Puchta, Holger Puchta Max-Planck-Institut für Biochemic, Abteilung ViroidforschungD-8033 Martinsried bei München, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Karla Ramm, Karla Ramm Max-Planck-Institut für Biochemic, Abteilung ViroidforschungD-8033 Martinsried bei München, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Heinz L. Sänger Heinz L. Sänger Max-Planck-Institut für Biochemic, Abteilung ViroidforschungD-8033 Martinsried bei München, FRG Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 16, Issue 17, 12 September 1988, Page 8708, https://doi.org/10.1093/nar/16.17.8708 Published: 12 September 1988 Article history Received: 08 August 1988 Published: 12 September 1988
Comparison of the Tamoxifen Regulated Chimeric Cre Recombinases MerCreMer and CreMer (1999)

The site-directed recombinase Cre can be employed to delete or assemble genes in the genome of living cells. We have constructed expression vectors for chimeric Cre recombinases carrying a mutated hormone binding domain either at the C-terminus only (CreMer) or at both the N and C-termini (MerCreMer). The chimeric Cre proteins can be activated by culturing transfected cells with 4-hydroxytamoxifen. In transfected embryonic stem (ES) cells, we compared the extent of recombination of a floxed gene with the expression levels of the chimeric Cre proteins. Our data demonstrate that the bulky MerCreMer protein is not less active than the CreMer protein. Thus, the tighter control and low background activity of the MerCreMer enzyme is not due to a generally low recombinase efficiency.
ASSOCIATION OF ANTIIDIOTYPIC ANTIBODY WITH SUCCESSFUL SECOND TRANSPLANT OF A KIDNEY SHARING HLA ANTIGENS WITH THE PREVIOUS HYPERACUTELY REJECTED FIRST KIDNEY (1989)

The evolution of HLA antibodies and autoantiidiotypic antibodies (AB2) were studied during an 18-month period in a patient who hyperacutely rejected an HLA-A2-positive kidney, but tolerated a second HLA-A2-positive kidney one year later. Following rejection of the first kidney, the patient's serum contained an HLA-A2 antibody that reacted with 100% of HLA-A2-positive panel cells. After several months, the HLA-A2 antibody activity was precipitously lost over a one-month period and could no longer be identified by sensitive lymphocytotoxicity procedures. Approximately one year later, the patient received a second HLA-A2-positive kidney that has survived for a 2-year period and was not associated with significant rejection episodes during the early posttransplantation period. Prior to and episodically following the second transplant, the patient's sera contained antiidiotypic-like antibodies that specifically inhibited HLA alloantibodies directed against HLA-A2. AB2, with specificity for a putative idiotype on HLA-A2 alloantibodies, existed concurrently with other HLA alloantibodies in the patient's serum that had not been lost over the course of several months. This case study demonstrates a temporal association between the loss of a specific HLA antibody and the development of an AB2 with inhibitory specificity for the antibody. The study also confirms that anamnestic responses to donor-specific antigens do not always occur in previously alloimmunized patients rechallenged with the same HLA antigens.
Cytotoxic and Antioxidant Activity of 4-Methylthio-3-butenyl Isothiocyanate from Raphanus sativus L. (Kaiware Daikon) Sprouts (2008)

There is high current interest in the chemopreventive potential of Brassica vegetables (cruciferae), particularly due to their content in glucosinolates (GL), which upon myrosinase hydrolysis release the corresponding isythiocyanates (ITC). Some ITCs, such as sulforaphane (SFN) from broccoli ( Brassica oleacea italica), have been found to possess anticancer activity through induction of apoptosis in selected cell lines, as well as indirect antioxidant activity through induction of phase II detoxifying enzymes. Japanese daikon ( Raphanus sativus L.) is possibly the vegetable with the highest per capita consumption within the Brassicaceae family. Thanks to a recently improved gram scale production process, it was possible to prepare sufficient amounts of the GL glucoraphasatin (GRH) as well as the corresponding ITC 4-methylthio-3-butenyl isothiocyanate (GRH-ITC) from its sprouts. This paper reports a study on the cytotoxic and apoptotic activities of GRH-ITC compared with the oxidized counterpart 4-methylsulfinyl-3-butenyl isothiocyanate (GRE-ITC) on three human colon carcinoma cell lines (LoVo, HCT-116, and HT-29) together with a detailed kinetic investigation of the direct antioxidant/radical scavenging ability of GRH and GRH-ITC. Both GRH-ITC and GRE-ITC reduced cell proliferation in a dose-dependent manner and induced apoptosis in the three cancer cell lines. The compounds significantly ( p < 0.05) increased Bax and decreased Bcl2 protein expression, as well as producing caspase-9 and PARP-1 cleavage after 3 days of exposure in the three cancer cell lines. GRH-ITC treatment was shown to have no toxicity with regard to normal human lymphocytes (-15 +/- 5%) in comparison with SFN (complete growth inhibition). GRH and GRH-ITC were able to quench the 2,2-diphenyl-1-picrylhydrazyl radical, with second-order rate constants of 14.0 +/- 2.8 and 43.1 +/- 9.5 M(-1) s(-1), respectively (at 298 K in methanol), whereas the corresponding value measured here for the reference antioxidant alpha-tocopherol was 425 +/- 40 M (-1) s (-1). GRH reacted with H2O2 and tert-butyl hydroperoxide in water (pH 7.4) at 37 degrees C, with rate constants of 1.9 +/- 0.3 x 10(-2) and 9.5 +/- 0.3 x 10(-4) M(-1) s (-1) (paralleling recently developed synthetic antioxidants) being quantitatively (>97%) converted to GRE. It is demonstrated that GRH-ITC has interesting antioxidant/radical scavenging properties, associated with a selective cytotoxic/apoptotic activity toward three human colon carcinoma cell lines, and very limited toxicity on normal human T-lymphocytes.
Sedentary Sphere (2015)

These data support the efficacy of the Sedentary Sphere for classification of posture from a wrist-worn accelerometer in adults. The approach is equally valid with data from both the GENEActiv and ActiGraph accelerometers.
Experience with anthracite - sand filters (1960)

The General Electric Company operates eight large filter plants for the Atomic Energy Commission at the Hanford works in the state of Washington. Because of the importance of water to the process, research and development on water treatment has been an important part of the overall Hanford research and development program. The research and development on water treatment has resulted in important capital and operating savings and in the production of better quality water. It is the purpose of this paper to present some of the information developed by the programs. 3 tabs.
The Anthrax Toxin Receptor 1 (ANTXR1) Is Enriched in Pancreatic Cancer Stem Cells Derived from Primary Tumor Cultures (2019)

Pancreatic ductal adenocarcinoma (PDAC) is currently the fourth leading cause of cancer-related mortality. Cancer stem cells (CSCs) have been shown to be the drivers of pancreatic tumor growth, metastasis, and chemoresistance, but our understanding of these cells is still limited by our inability to efficiently identify and isolate them. While a number of markers capable of identifying pancreatic CSCs (PaCSCs) have been discovered since 2007, there is no doubt that more markers are still needed. The anthrax toxin receptor 1 (ANTXR1) was identified as a functional biomarker of triple-negative breast CSCs, and PDAC patients stratified based on <i>ANTXR1</i> expression levels showed increased mortality and enrichment of pathways known to be necessary for CSC biology, including TGF-<i>β</i>, NOTCH, Wnt/<i>β</i>-catenin, and IL-6/JAK/STAT3 signaling and epithelial to mesenchymal transition, suggesting that ANTXR1 may represent a putative PaCSC marker. In this study, we show that ANTXR1<sup>+</sup> cells are not only detectable across a panel of 7 PDAC patient-derived xenograft primary cultures but ANTXR1 expression significantly increased in CSC-enriched 3D sphere cultures. Importantly, ANTXR1<sup>+</sup> cells also coexpressed other known PaCSC markers such as CD44, CD133, and autofluorescence, and ANTXR1<sup>+</sup> cells displayed enhanced CSC functional and molecular properties, including increased self-renewal and expression of pluripotency-associated genes, compared to ANTXR1<sup>-</sup> cells. Thus, this study validates ANTXR1 as a new PaCSC marker and we propose its use in identifying CSCs in this tumor type and its exploitation in the development of CSC-targeted therapies for PDAC.
A Conversation about Land Cancellation and Release with H. Murphey “Murf” McCloy (2009)

Land Cancellation and Release in mine action is looked at by many experts as the next logical step to the safe and time-effective return of mined areas. This interview examines the benefits of the land-release method and addresses its criticisms.
Multi-View Clustering via Deep Matrix Factorization (2017)

Multi-View Clustering (MVC) has garnered more attention recently since many real-world data are comprised of different representations or views. The key is to explore complementary information to benefit the clustering problem. In this paper, we present a deep matrix factorization framework for MVC, where semi-nonnegative matrix factorization is adopted to learn the hierarchical semantics of multi-view data in a layer-wise fashion. To maximize the mutual information from each view, we enforce the non-negative representation of each view in the final layer to be the same. Furthermore, to respect the intrinsic geometric structure in each view data, graph regularizers are introduced to couple the output representation of deep structures. As a non-trivial contribution, we provide the solution based on alternating minimization strategy, followed by a theoretical proof of convergence. The superior experimental results on three face benchmarks show the effectiveness of the proposed deep matrix factorization model.
The novel β‐secretase inhibitor KMI‐429 reduces amyloid β peptide production in amyloid precursor protein transgenic and wild‐type mice (2005)

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by the accumulation of amyloid plaques and neurofibrillary tangles in the brain. The major component of the plaques, amyloid beta peptide (Abeta), is generated from amyloid precursor protein (APP) by beta- and gamma-secretase-mediated cleavage. Because beta-secretase/beta-site APP cleaving enzyme 1 (BACE1) knockout mice produce much less Abeta and grow normally, a beta-secretase inhibitor is thought to be one of the most attractive targets for the development of therapeutic interventions for AD without apparent side-effects. Here, we report the in vivo inhibitory effects of a novel beta-secretase inhibitor, KMI-429, a transition-state mimic, which effectively inhibits beta-secretase activity in cultured cells in a dose-dependent manner. We injected KMI-429 into the hippocampus of APP transgenic mice. KMI-429 significantly reduced Abeta production in vivo in the soluble fraction compared with vehicle, but the level of Abeta in the insoluble fraction was unaffected. In contrast, an intrahippocampal injection of KMI-429 in wild-type mice remarkably reduced Abeta production in both the soluble and insoluble fractions. Our results indicate that the beta-secretase inhibitor KMI-429 is a promising candidate for the treatment of AD.
Metabolism of [14C]acrolein (Magnacide H® herbicide): Nature and magnitude of residues in freshwater fish and shellfish (1998)

Abstract Arolein, anaquaticherbicide, is appliedas MAGNAIDE H® herbicide directly into the water of irrigation canals at concentrations of 1 to 15 ppm. Two representative freshwater fish species, the bluegill sunfish (Lepomis macrochirus) and the channel catfish (Ictalurus punctatus), and two representative freshwater shellfish species, a unionacean clam (Elliptio complanata) and the northern crayfish (Orconectes virilis), were exposed separately to two applications of [14C]acrolein, with 7 d between the two dosings. The nominal water concentrations were 0.02 and 0.1 ppm acrolein for fish and shellfish, respectively. Exposures were terminated 1 d after the second application. Metabolites were identified from the edible tissues of each test species; there was very little similarity in the metabolism of acrolein between them. The most notable observation was that acrolein was never detected in any of the tissues sampled. Glycidol, glycerol, 1,3-propanediol, and glyceric acid were the major metabolites found in the catfish, crayfish, bluegill, and clams, respectively. This study demonstrated that these test species, when exposed under static conditions to [14C]acrolein, are able to metabolize the parent compound and its residues in their edible tissues. The metabolism of [14C]acrolein is so rapid in the edible tissues of these species that neither acrolein nor its major oxidative and reductive metabolites, acrylic acid and allyl alcohol, respectively, were detected.
Site-Selective Doping of Dyes into Polystyrene-block-Poly(4-vinyl pyridine) Diblock Copolymer Films and Selective Laser Ablation of the Dye-Doped Films (2007)

The site-selective doping of dyes into spin-cast thin films with different phase separation structures of polystyrene-block-poly(4-vinyl pyridine) (PS-b-P4VP) diblock copolymers and the dopant-induced laser ablation of the dye-doped films fabricated were investigated. The selective doping of dyes into P4VP parts was carried out by immersing the films in a methanol solution containing the dyes, in which the driving force was induced by hydrogen bonds between the nitrogen atoms of pyridyl groups and the carboxylic groups of the dyes. Schematic models for the adsorption process of the dyes in the methanol solution were discussed. Dopant-induced laser ablation was carried out on the dye-doped block copolymer films, resulting in the ablation of dye-doped P4VP parts only. Factors affecting selective ablation, such as dyes, the morphologies of phase separation structures, the number-average molecular weights of the diblock copolymers, and irradiation environments, were discussed.
Distinct alterations in lineage committed progenitor cells exist in the peripheral blood of patients with rheumatoid arthritis and primary Sjogren's syndrome. (1996)

Distinct alterations in the distribution of PB progenitors are present in RA and primary SS. Since progenitor cells retain a proliferative capacity, their infiltration into the synovial/glandular environment may contribute to the accumulation of inflammatory cells within these sites. We propose that PB progenitors enter the diseased microenvironment through similar mechanisms as mature hematopoietic elements.
A hybrid parametric-waveform approach to bit stream scalable audio coding (2005)

In this paper we present a wideband (44.1 kHz sampling rate) audio and speech coder that combines two different strategies, namely, parametric and waveform coding. It is shown how this approach can be used to design a layered bit stream scalable coder offering a wide variety of decoding bit rates with little scalability loss. Moreover, the bit rates associated with the different layers are competitive, in terms of quality, to those of standardized coders (MP3, AAC) tuned at a particular bit rate.