gebhart/patrepeval huggingface dataset:
v-src Induces Prostaglandin Synthase 2 Gene Expression by Activation of the c-Jun N-terminal Kinase and the c-Jun Transcription Factor (1995)

A consensus cyclic AMP response element (CRE) in the murine prostaglandin synthase-2 (PGS2) promoter is essential for pgs2 gene expression induced by pp60v-src, the v-src oncogene product. In this study, we investigate (i) the transcription factors active at the PGS2 "CRE site" in response to v-src activation and (ii) the signal transduction pathways by which pp60v-src activates these transcription factors. Transient transfection assays with pgs2 promoter/luciferase reporter chimeric genes suggest that c-Jun mediates v-src-induced pgs2 gene expression. Antibody supershift experiments demonstrate that c-Jun can participate in a complex with the pgs2 promoter CRE site. Moreover, in vitro immuno-complex assays demonstrate that pp60v-src expression strongly activates c-Jun N-terminal kinase (JNK1) enzyme activity. Serines 63 and 73, the sites of c-Jun phosphorylation by JNK, are essential for v-src-induced, pgs2 promoter-mediated luciferase expression. Cotransfection studies with plasmids expressing wild-type JNK, dominant-negative JNK, and dominant-negative MEKK-1 confirm that activation of the Ras/MEKK-1/JNK/c-Jun pathway is required for v-src-induced pgs2 gene expression. Overexpression of either wild-type ERK-1 or ERK-2 proteins also potentiate v-src-mediated luciferase expression driven by the pgs2 promoter, and expression of dominant-negative mutants of ERK-1, ERK-2, or Raf-1 attenuate this response. Thus, in response to v-src expression, a Ras/MEKK-1/JNK signal transduction pathway activating c-Jun and a Ras/Raf-1/ERK pathway converge to mediate pgs2 gene expression via the CRE site in the pgs2 promoter.
Effect of UV radiation on interferon, immunoglobulins and complement components in serum from healthy individuals. (1987)

An infectivity inhibition micro-method was used to detect interferon (IFN) in sera from 20 healthy individuals before and after UVA irradiation, and in sera from 22 healthy individuals before and after UVB irradiation. The serum levels of IFN, immunoglobulins (Ig) and complement components (C) were examined before irradiation, after 10 and 20 exposures, and 4 weeks after irradiation. The median serum IFN level in both the UVA and the UVB group increased significantly during irradiation, most markedly during the 10 first exposures. Four weeks after irradiation the median IFN level was reduced in both groups, but had not reached the pre-irradiation levels. The IFN level was unchanged or reduced in 2 individuals in the UVA group and in 5 individuals in the UVB group. Characterization experiments indicated the presence of both acid-stable and acid-labile IFN-alpha and IFN-gamma. The mean serum IgG concentration was significantly reduced 4 weeks after UVA irradiation, whereas IgA, IgM, C3 and C4 were not affected. The mean serum concentrations of IgG and C4 were significantly reduced 4 weeks after UVB irradiation, whereas the mean concentrations of IgM and C3 were significantly increased. The concentration of IgA was not affected.
The chemistry of polynuclear compounds. Part XXVI. Products of the pyrolysis of dodecacarbonyl-triangulo-triruthenium and -triosmium (1975)

Pyrolysis of [Ru3(CO)12] in a sealed evacuated tube gives [Ru6(CO)17C] thereby clearly establishing that the ‘isolated’ carbon atom is derived from reduction of a CO group. Pyrolysis of [Os3(CO)12] under similar conditions leads to formation of polynuclear osmium carbonyl species based on five to eight osmium atoms, viz. [Os5(CO)16], [Os5(CO)15C], [Os6(CO)18], [Os7(CO)21], [Os8(CO)23], and [Os8(CO)21C]. The probable structures of these polynuclear complexes are discussed.
Amino acid sequence of locust neuroparsins (1989)

Neuroparsins A and B were isolated from the nervous part of the corpus cardiaca of Locusta migratoria via a two-step purification procedure. Both consist of two polypeptide chains linked by disulfide bridges. The N-terminal sequence of both native neuroparsins was determined: the N-terminal end of neuroparsin B was unique while that of neuroparsin A showed three different sequences. These sequences were that of neuroparsin B and two others having five and two extra N-terminal residues. Neuroparsin B was found as a homodimer and the complete sequence of the monomer, determined from peptide fragments generated by treatment with cyanogen bromide and endoprotease Glu-C, comprises 78 residues.
An in‐gel digestion procedure that facilitates the identification of highly hydrophobic proteins by electrospray ionization‐mass spectrometry analysis (2005)

A procedure is described for in-gel tryptic digestion of proteins that allows the direct analysis of eluted peptides in electrospray ionization (ESI) mass spectrometers without the need of a postdigestion desalting step. It is based on the following principles: (a) a thorough desalting of the protein in-gel before digestion that takes advantage of the excellent properties of acrylamide polymers for size exclusion separations, (b) exploiting the activity of trypsin in water, in the absence of inorganic buffers, and (c) a procedure for peptide extraction using solvents of proven efficacy with highly hydrophobic peptides. Quality of spectra and sequence coverage are equivalent to those obtained after digestion in ammonium bicarbonate for hydrophilic proteins detected with Coomassie blue, mass spectrometry-compatible silver or imidazole-zinc but are significantly superior for highly hydrophobic proteins, such as membrane proteins with several transmembrane domains. ATPase subunit 9 (GRAVY 1.446) is a membrane protein channel, lipid-binding protein for which both the conventional in-gel digestion protocol and in solution digestion failed. It was identified with very high sequence coverage. Sample handling after digestion is notably simplified as peptides are directly loaded into the ESI source without postdigestion processing, increasing the chances for the identification of hydrophobic peptides.
Acute Toxicology Testing: Perspectives and Horizons (1988)

Acute toxicology testing constitutes the first line of defense against potentially dangerous chemicals. This book provides a detailed presentation of protocols for each of the common designs, reviews their development and objectives, discusses the types of data they generate, and examines the current status of alternative test designs and models. F
Independent effects of weight and mass on plantar flexor activity during walking: implications for their contributions to body support and forward propulsion (2008)

The ankle plantar flexor muscles, gastrocnemius (Gas) and soleus (Sol), have been shown to play important roles in providing body support and forward propulsion during human walking. However, there has been disagreement about the relative contributions of Gas and Sol to these functional tasks. In this study, using independent manipulations of body weight and body mass, we examined the relative contribution of the individual plantar flexors to support and propulsion. We hypothesized that Gas and Sol contribute to body support, whereas Sol is the primary contributor to forward trunk propulsion. We tested this hypothesis by measuring muscle activity while experimentally manipulating body weight and mass by 1) decreasing body weight using a weight support system, 2) increasing body mass alone using a combination of equal added trunk load and weight support, and 3) increasing trunk loads (increasing body weight and mass). The rationale for this study was that muscles that provide body support would be sensitive to changes in body weight, whereas muscles that provide forward propulsion would be sensitive to changes in body mass. Gas activity increased with added loads and decreased with weight support but showed only a small increase relative to control trials when mass alone was increased. Sol activity showed a similar increase with added loads and with added mass alone and decreased in early stance with weight support. Therefore, we accepted the hypothesis that Sol and Gas contribute to body support, whereas Sol is the primary contributor to forward trunk propulsion.
Peyronie's disease revisited. (1979)

Various treatment modalities have been suggested for Peyronie's disease. Varying degrees of success have been reported. Certain numbers of cases will have spontaneous remission of the disease process. The treatment, however, should begin as early as possible and recognition should be achieved earlier. The injection technique utilizing a high-pressure metal syringe or syringe jacket with screw-type barrell is still the most successful for the "soft fibrosis" lesions. This was reported about twenty-five years ago. A small number of patients treated by plaque excision and full thickness skin graft replacement have also shown good results. The type of lesion present best dictates the proper treatment.
Identification of a Bifunctional Lipopolysaccharide Sialyltransferase in Haemophilus influenzae (2006)

The lipopolysaccharide (LPS) of non-typeable Haemophilus influenzae (NTHi) can be substituted at various positions by N-acetylneuraminic acid (Neu5Ac). LPS sialylation plays an important role in pathogenesis. The only LPS sialyltransferase characterized biochemically to date in H. influenzae is Lic3A, an alpha-2,3-sialyltransferase responsible for the addition of Neu5Ac to a lactose acceptor (Hood, D. W., Cox, A. D., Gilbert, M., Makepeace, K., Walsh, S., Deadman, M. E., Cody, A., Martin, A., Månsson, M., Schweda, E. K., Brisson, J. R., Richards, J. C., Moxon, E. R., and Wakarchuk, W. W. (2001) Mol. Microbiol. 39, 341-350). Here we describe a second sialyltransferase, Lic3B, that is a close homologue of Lic3A and present in 60% of NTHi isolates tested. A recombinant form of Lic3B was expressed in Escherichia coli and purified by affinity chromatography. We used synthetic fluorescent acceptors with a terminal lactose or sialyllactose to show that Lic3B has both alpha-2,3- and alpha-2,8-sialyltransferase activities. Structural analysis of LPS from lic3B mutant strains of NTHi confirmed that only monosialylated species were detectable, whereas disialylated species were detected upon inactivation of lic3A. Furthermore, introduction of lic3B into a lic3B-deficient strain background resulted in a significant increase in sialylation in the recipient strain. Mass spectrometric analysis of LPS indicated that glycoforms containing two Neu5Ac residues were evident that were not present in the LPS of the parent strain. These findings characterize the activity of a second sialyltransferase in H. influenzae, responsible for the addition of di-sialic acid to the LPS. Modification of the LPS by di-sialylation conferred increased resistance of the organism to the killing effects of normal human serum, as compared with mono-sialylated or non-sialylated species, indicating that this modification has biological significance.
Ficin. I. Its isolation and characterization (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFicin. I. Its isolation and characterizationPaul T. Englund, Te Piao. King, Lyman C. Craig, and A. WaltiCite this: Biochemistry 1968, 7, 1, 163–175Publication Date (Print):January 1, 1968Publication History Published online1 May 2002Published inissue 1 January 1968https://pubs.acs.org/doi/10.1021/bi00841a021https://doi.org/10.1021/bi00841a021research-articleACS PublicationsRequest reuse permissionsArticle Views486Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Isolated postaxial polydactyly type B with mosaicism of a submicroscopic unbalanced translocation leading to an extended phenotype in offspring (2003)

Postaxial polydactyly (PAP) is characterized by the presence of one or more extra ulnar or fibular digits or parts of it. PAP type B presents frequently as a skin tag on the hand(s). It is usually an isolated malformation, but in 6.6% it is associated with other congenital abnormalities, mostly well recognizable syndromes. We present a male with PAP-B only and his daughter with an extended phenotype including mental retardation and minor dysmorphisms. Both share a cytogenetically balanced t(4;7)(p15.2;q35), present in mosaicism in the father. We found microdeletions associated with the breakpoints. The chromosomal regions described here have not been previously associated with the PAP-B phenotype. We present the first case of an individual with isolated PAP-B and a submicroscopic chromosome abnormality.
Business Traveler Fitness (2008)

Occupational and environmental health nurses can counsel employees who travel about remaining healthy and fit on the road.
The effect of ingestion of amino acids, glucose and fat on circulating neurotensin‐like immunoreactivity (NTLI) in man (1979)

The effect of ingestion of amino acids (Vamin N), glucose, and fat (Intralipid) on the concentration of neurotensin-like immunoreactivity (NTLI) in plasma was determined in 6 healthy male volunteers. After ingestion of Intralipid (55 ml, 200 mgxml-1) there was a significant increase in the plasma concentration of NTLI. The calculated integrated total NTLI response (6.7 +/- 2.5 nM over 180 min) was statistically significant. Although isocaloric amounts of Vamin and glucose also increased the plasma concentration of NTLI, these increases were not statistically significant. The data indicate that fat is an important stimulus for the release of NTLI from the small intestine. It is suggested that neurotensin, or a metabolite, in blood may be a hormone involved in the postprandial inhibition of gastric motility and gastric acid secretion elicited from the small intestine.
Large-Signal Behavior of Junction Transistors (1954)

In the consideration of the junction transistor as a switch there are three characteristics of primary interest, the open impedance, the closed impedance, and the switching-time. A generalized two-terminal-pair theory of junction transistors is presented which is applicable, on a dc basis, in all regions of operation. Using this theory, the open and closed impedances of the transistor switch are expressible in terms of easily measurable transistor parameters. For the ideal transistor these parameters are the saturation currents of the emitter and collector junctions and the normal and inverted alphas. The transition of the transistor switch from open to closed, or vice versa, is discussed, including the effects of minority carrier storage. This transition can be expressed in analytic form in terms of the alphas and the normal and inverted alpha cut-off frequencies.
Electrical Stimuli Release ATP to Increase GLUT4 Translocation and Glucose Uptake via PI3Kγ-Akt-AS160 in Skeletal Muscle Cells (2012)

Skeletal muscle glucose uptake in response to exercise is preserved in insulin-resistant conditions, but the signals involved are debated. ATP is released from skeletal muscle by contractile activity and can autocrinely signal through purinergic receptors, and we hypothesized it may influence glucose uptake. Electrical stimulation, ATP, and insulin each increased fluorescent 2-NBD-Glucose (2-NBDG) uptake in primary myotubes, but only electrical stimulation and ATP-dependent 2-NBDG uptake were inhibited by adenosine-phosphate phosphatase and by purinergic receptor blockade (suramin). Electrical stimulation transiently elevated extracellular ATP and caused Akt phosphorylation that was additive to insulin and inhibited by suramin. Exogenous ATP transiently activated Akt and, inhibiting phosphatidylinositol 3-kinase (PI3K) or Akt as well as dominant-negative Akt mutant, reduced ATP-dependent 2-NBDG uptake and Akt phosphorylation. ATP-dependent 2-NBDG uptake was also inhibited by the G protein βγ subunit-interacting peptide βark-ct and by the phosphatidylinositol 3-kinase-γ (PI3Kγ) inhibitor AS605240. ATP caused translocation of GLUT4myc-eGFP to the cell surface, mechanistically mediated by increased exocytosis involving AS160/Rab8A reduced by dominant-negative Akt or PI3Kγ kinase-dead mutants, and potentiated by myristoylated PI3Kγ. ATP stimulated 2-NBDG uptake in normal and insulin-resistant adult muscle fibers, resembling the reported effect of exercise. Hence, the ATP-induced pathway may be tapped to bypass insulin resistance.
Activity of CeO <sub> <i>x</i> </sub> and TiO <sub> <i>x</i> </sub> Nanoparticles Grown on Au(111) in the Water-Gas Shift Reaction (2007)

The high performance of Au-CeO2 and Au-TiO2 catalysts in the water-gas shift (WGS) reaction (H2O + CO-->H2 + CO2) relies heavily on the direct participation of the oxide in the catalytic process. Although clean Au(111) is not catalytically active for the WGS, gold surfaces that are 20 to 30% covered by ceria or titania nanoparticles have activities comparable to those of good WGS catalysts such as Cu(111) or Cu(100). In TiO(2-x)/Au(111) and CeO(2-x)/Au(111), water dissociates on O vacancies of the oxide nanoparticles, CO adsorbs on Au sites located nearby, and subsequent reaction steps take place at the metal-oxide interface. In these inverse catalysts, the moderate chemical activity of bulk gold is coupled to that of a more reactive oxide.
Process optimization of buried Ag^+–Na^+ ion-exchanged waveguides: theory and experiment (1988)

We report optimization of the processing conditions for fabrication of single-mode Ag(+)-Na(+) ion-exchanged waveguides in a soda-lime glass. The surface-index change, the mobility and diffusion coefficient of Ag(+) have been measured at low melt concentrations and the results used for calculation of the index profile using a new simple numerical method for solving the diffusion equation in a two-step process where the index profile is buried by applying an electric field in the second step. The calculated profile is in agreement with that measured. This correlation allows solution of the Helmholtz equation for given process parameters. Single-mode waveguides with desired mode-field distribution have been designed and fabricated with optimized performance and fiber compatibility at 1.3 microm.
Community Work with Immigrant Groups (1990)

This paper is based on the findings of an evaluation of a community work project in a multi-ethnic housing estate in Villawood, N.S.W. 1987–1988. Analysis of the work reveals the importance of extensive personal contacting and ethnic specific work, before attempts are made to organise cross cultural groups which can work on common issues.
Stage-specific alterations in murine B lymphopoiesis with age (1996)

Although it is reported that B lymphopoiesis declines with age, the precursor stage(s) affected and the age of onset are ambiguous. Each progressive phase of B cell differentiation has distinct requirements; therefore, precise identification of the stage(s) that decline would yield insight into the age-related mechanisms affecting humoral immunity. We analysed the composition of B lineage cells of mice 1, 4, 12 and 24 months of age using flow cytometry. Numbers of prepro-B and pro-B cells were unchanged, and a profound decrease occurred only in the numbers of pre-B cells. This decrease occurred in two distinct phases: between 1 and 4 months and between 12 and 24 months. Notably, the numbers of newly formed B cells did not decline in parallel, suggesting that mechanisms are established to overcome the deficiency of pre-B cells. Since the age-related changes are limited to the pre-B cell stage, we hypothesized that the impairment acts at the pro-B to pre-B transition. We therefore evaluated whether the pro-B cells or the supporting stromal cells, which are necessary for normal progression of this stage, changed with age. The ability of pro-B cells to proliferate in the presence of stromal cells was reduced by 24 months of age, as was the ability of the stromal cells to support pro-B cell proliferation. In contrast, the ability to mature into IgM+ cells was unchanged. Thus, strategies that supplement the stromal environment may enhance B lymphopoiesis in aged animals.
Experimental meningococcal infection in neonatal animals: models for mucosal invasiveness (1984)

A more complete understanding of meningococcal disease has been hampered by lack of an appropriate animal model. We subjected 5-day-old guinea pigs, rats, and mice to intranasal challenge with meningococci and we measured rates of bacteremia as a marker of mucosal invasion. After a single intranasal instillation of 10(7) serotype 2 meningococci, positive blood cultures were found in 0% of guinea pigs, 16% of rats, and 39% of mice, and so mice were used for further studies. Death occurred in 4% of mice and was associated with a purulent leptomeningitis and ventriculitis. Forty percent of mice had nasopharyngeal colonization which increased to 65% with repeated injections. Carrier strains were avirulent, a nonserotype 2 disease strain had low invasiveness, and serotype 2 strains were most virulent. Iron dextran increased rates of bacteremia after challenge with serotype 2 strains. Adult animals were not susceptible to bacteremia after intranasal challenge. The neonatal mouse model fulfills most of the criteria for an appropriate experimental model of meningococcal disease.
Wireless sensor networks - Part I: topology and design issues (2006)

Wireless sensor networks (WSNs) have a wide range of invaluable civil and military applications. Nodes in WSNs act as information sources, sensing and collecting data samples from their environment. They perform routing functions, creating multi-hop wireless networking fabric that conveys data samples to other sensor nodes. A number of important performance measures have to be considered in designing WSN. Among these, energy efficiency and adaptive medium access control (MAC) and scalability. In part I of this series, we review a number of topologies and design techniques used in the deployment of WSNs. In particular, we cover the star, mesh and hybrid topologies. Factors influencing the design of WSNs include power consumption, scalability, operating environment, transmission media, fault tolerance/reliability, and topology changes. We also present a number of WSNs communication architectures
Kinetic Nephelometric Procedure for Measurement of Amylase Activity in Serum (1972)

Abstract We developed a kinetic procedure for determination of amylase activity in serum by use of nephelometric measurements. Light scattering from the substrate, a stable suspension of starch, is decreased as amylase hydrolyzes the starch to soluble fragments. Values obtained for serum amylase correlate closely with those determined by a method in which a starch-dye complex is used. Precision of the new procedure is 3.4% (relative standard deviation). Units of amylase activity can either be expressed in terms of milligrams of starch consumed or converted to more conventional amylase units.