gebhart/patrepeval huggingface dataset:
Mobile manifestations of alertness (2016)

Our body clock causes considerable variations in our behavioral, mental, and physical processes, including alertness, throughout the day. While much research has studied technology usage patterns, the potential impact of underlying biological processes on these patterns is under-explored. Using data from 20 participants over 40 days, this paper presents the first study to connect patterns of mobile application usage with these contributing biological factors. Among other results, we find that usage patterns vary for individuals with different body clock types, that usage correlates with rhythms of alertness, that app use features such as duration and switching can distinguish periods of low and high alertness, and that app use reflects sleep interruptions as well as sleep duration. We conclude by discussing how our findings inform the design of biologically-friendly technology that can better support personal rhythms of performance.
Activity‐based kinase profiling of approved tyrosine kinase inhibitors (2012)

The specificities of nine approved tyrosine kinase inhibitors (imatinib, dasatinib, nilotinib, gefitinib, erlotinib, lapatinib, sorafenib, sunitinib, and pazopanib) were determined by activity-based kinase profiling using a large panel of human recombinant active kinases. This panel consisted of 79 tyrosine kinases, 199 serine/threonine kinases, three lipid kinases, and 29 disease-relevant mutant kinases. Many potential targets of each inhibitor were identified by kinase profiling at the K(m) for ATP. In addition, profiling at a physiological ATP concentration (1 mm) was carried out, and the IC(50) values of the inhibitors against each kinase were compared with the estimated plasma-free concentration (calculated from published pharmacokinetic parameters of plasma C(trough) and C(max) values). This analysis revealed that the approved kinase inhibitors were well optimized for their target kinases. This profiling also implicates activity at particular off-target kinases in drug side effects. Thus, large-scale kinase profiling at both K(m) and physiological ATP concentrations could be useful in characterizing the targets and off-targets of kinase inhibitors.
A molecular signature in blood identifies early Parkinson’s disease (2012)

The findings provide evidence on the ability of a five-gene panel to diagnose early/mild PD, with a possible diagnostic value for detection of asymptomatic PD before overt expression of the disorder.
Tracking Vital Signs During Sleep Leveraging Off-the-shelf WiFi (2015)

Tracking human vital signs of breathing and heart rates during sleep is important as it can help to assess the general physical health of a person and provide useful clues for diagnosing possible diseases. Traditional approaches (e.g., Polysomnography (PSG)) are limited to clinic usage. Recent radio frequency (RF) based approaches require specialized devices or dedicated wireless sensors and are only able to track breathing rate. In this work, we propose to track the vital signs of both breathing rate and heart rate during sleep by using off-the-shelf WiFi without any wearable or dedicated devices. Our system re-uses existing WiFi network and exploits the fine-grained channel information to capture the minute movements caused by breathing and heart beats. Our system thus has the potential to be widely deployed and perform continuous long-term monitoring. The developed algorithm makes use of the channel information in both time and frequency domain to estimate breathing and heart rates, and it works well when either individual or two persons are in bed. Our extensive experiments demonstrate that our system can accurately capture vital signs during sleep under realistic settings, and achieve comparable or even better performance comparing to traditional and existing approaches, which is a strong indication of providing non-invasive, continuous fine-grained vital signs monitoring without any additional cost.
<i>O</i>‐Trifluoromethylation of <i>N</i>,<i>N</i>‐Disubstituted Hydroxylamines with Hypervalent Iodine Reagents (2014)

Abstract A mild trifluoromethylation reaction of N , N ‐disubstituted hydroxylamines that is tolerant towards a variety of functional groups, including nitriles, alcohols, ketones, esters, amides, imides, and nitrogen heterocycles, is reported. The key feature of this reaction is the activation of the CF 3 reagent with either trimethylsilyl triflate or LiClO 4 and partial or full deprotonation of the substrate with tetramethylguanidine or lithium diisopropylamide. Products were obtained in up to 80 % yield. Preliminary mechanistic studies suggested that the reaction follows a radical pathway in which the deprotonated hydroxylamine and a Lewis or Brønsted acid activated CF 3 reagent engages in a single‐electron‐transfer step to generate a pair of radicals that recombine to afford the desired product. The trifluoromethylation procedure was successfully used in the modification of secondary nitrogen groups of pharmaceutically relevant targets (Fluoxetine and Mefloquine), which afforded new derivatives containing a novel N ‐trifluoromethoxy moiety.
Transendothelial Migration of CD34+ and Mature Hematopoietic Cells: An In Vitro Study Using a Human Bone Marrow Endothelial Cell Line (1997)

To study the role of bone marrow endothelial cells (BMEC) in the regulation of hematopoietic cell trafficking, we have designed an in vitro model of transendothelial migration of hematopoietic progenitor cells and their progeny. For these studies, we have taken advantage of a human BMEC-derived cell line (BMEC-1), which proliferates independent of growth factors, is contact inhibited, and expresses adhesion molecules similar to BMEC in vivo. BMEC-1 monolayers were grown to confluency on 3 microns microporous membrane inserts and placed in 6-well tissue culture plates. Granulocytecolony stimulating factor (G-CSF)-mobilized peripheral blood CD34+ cells were added to the BMEC-1 monolayer in the upper chamber of the 6-well plate. After 24 hours of coincubation, the majority of CD34+ cells remained nonadherent in the upper chamber, while 1.6 +/- 0.3% of the progenitor cells had transmigrated. Transmigrated CD34 cells expressed a higher level of CD38 compared with nonmigrating CD34+ cells and may therefore represent predominantly committed progenitor cells. Accordingly, the total plating efficiency of the transmigrated CD34+ cells for lineage-committed progenitors was higher (14.0 +/- 0.1 v 7.8% +/- 1.5%). In particular, the plating efficiency of transmigrated cells for erythroid progenitors was 27-fold greater compared with nonmigrating cells (8.0% +/- 0.8% v 0.3% +/- 0.1%) and 5.5-fold compared with unprocessed CD34+ cells (2.2% +/- 0.4%). While no difference in the expression of the beta 1-integrin very late activation antigen (VLA)-4 and beta 2-integrin lymphocyte function-associated antigen (LFA)-1 was found, L-selectin expression on transmigrated CD34+ cells was lost, suggesting that shedding had occurred during migration. The number of transmigrated cells was reduced by blocking antibodies to LFA-1, while L-selectin and VLA-4 antibodies had no inhibitory effect. Continuous coculture of the remaining CD34+ cells in the upper chamber of the transwell inserts resulted in proliferation and differentiation into myeloid and megakaryocytic cells. While the majority of cells in the upper chamber comprised proliferating myeloid precursors such as promyelocytes and myelocytes, only mature monocytes and granulocytes were detected in the lower chamber. In conclusion, BMEC-1 cells support transmigration of hematopoietic progenitors and mature hematopoietic cells. Therefore, this model may be used to study mechanisms involved in mobilization and homing of CD34+ cells during peripheral blood progenitor cell transplantation and trafficking of mature hematopoietic cells.
Carbon Dioxide-in-Brine Foams at High Temperatures and Extreme Salinities Stabilized with Silica Nanoparticles (2017)

The stabilization of carbon dioxide-in-water (C–W) foams with nanoparticles (NPs) becomes highly challenging as the temperature and salinity increase, particularly for divalent ions, as the nanoparticles often aggregate in the brine phase. For silica nanoparticles with a medium coverage (MC) and high coverage (HC) of organic ligands, the hydrophilic–CO2-philic balance (HCB) was found to be in the appropriate range to produce a large reduction in the C–W interfacial tension (IFT). Furthermore, the nanoparticles were colloidally stable in concentrated brine (15% total dissolved solids, TDS) up to 80 °C. With these interfacially active nanoparticles, C–W foams were stabilized with apparent foam viscosities up to 35 cP and foam textures with bubble sizes on the order of 40 μm at various gas fractional flows (foam qualities) in beadpack experiments. At the foam quality where the apparent viscosity was a maximum (transition quality) in the beadpack, we also produced CO2 foams in Boise and Berea cores versus temperature with apparent viscosities up to 26 cP at 70 °C and 15% TDS and hysteresis in the apparent viscosity versus the interstitial velocity. The reductions in the IFT and foam strength at elevated temperature were modestly larger for the HC nanoparticles than for the MC nanoparticles but were low for the low-coverage case. Given that the interfacial adsorption increased with salinity up to 15% TDS, the screening of the charge helped drive the particles from the brine phase to the interface, which was necessary to stabilize the foams.
A bacteriological investigation of artificial mineral waters (1893)

The points investigated were-1.The source of the water and the methods employed in its 2. The number and nature of the micro-organisms present in 3. The action of CO, on the microbes present in artificial waters.4. The action of CO, under varying pressures on certain pathogenic organisms.The mineral waters examined were either simple a6rated or alkaline waters, obtained, by the courtesy of various leading London makers, direct from the factories, and were selected to show what was the influence of variation in source of supply, or subsequent treatment on the bacteriology of the finished waters.purification.mineral waters derived from various sources. I. THE SOURCES AND METHODS OF PURIFICATION.It was found that the waters employed in London might be grouped into four chief varieties, according to the original sources of the water or the methods of purification employed before a6ration.These groups are-A.River or deep-well water as supplied by one of the London water companies.The water was used direct from the mains or after charcoal filtration and subjection to a softening process.B. Artesian-well water from private wells situated in different parts of the London district.This water was generally filtered through either animal or block charcoal.This was water derived from one of the water companies, distilled under pressure, the pressure varying from 20 to 35 lbs.per square inch.Occasionally the water was distilled a t 100" C. The distilled water after condensation, which was effected in tin-lined condensers, was filtered through either animal or wood charcoal, in order to remove the peculiar mawkish flavour of distilled water.The charcoal apparently acts by oxidation of a volatile organic compound which, as well as the want of aeration, is the cause of the distinctive taste.The water supplied by a water company after filtration through spongy iron filters was treated with sodium carbonate (a softening process) and potassium permanganate, and then boiled by steam coils, in covered tanks.The openings to the tanks were protected by cotton-wool plugs in inverted U-tubes.The water was then syphoned off and filtered through pounded glass into another covered and protected tank, from which it passed to the acrating machines.The waters employed, then, are very varied, and include river, deepwell, artesian-well, distilled and boiled water ; and as these varieties are known to be biologically very different, it might be expected that the C. Distilled water. D. Boiled water.' finished agrated waters prepared from these varied sources would present strongly marked differences.
Prediction of perceived phonetic distance from short-term spectra—a first step (1981)

Some models of speech perception (and many speech recognition devices) posit a process whereby short-term speech spectra are compared with a set of reference templates. These schemes will succeed only to the extent that metrics can be found that are (1) sensitive to phonetically relevant spectral differences such as those caused by formant frequency changes, and (2) relatively insensitive to phonetically irrelevant spectral differences associated with a change in speaker identity or recording conditions. Judgements of phonetic distance between pairs of static synthetic vowels and fricatives have been collected in which the stimulus ensemble included formant frequency changes and a number of acoustic changes that turn out to have little phonetic relevance (e.g., spectral tilt, relative formant amplitudes, high-pass and low-pass filtering). These data can be used to evaluate a spectral distance metric. For example, distance calculations based on the sum-of-squares of differences in critical-band filter bank outputs and those based on the linear-prediction residual correlate poorly with the vowel distance-judgement data. A metric based on spectral slope differences near the peaks in the criticalband spectra to be compared can be made to correlate very well (0.95) with the perceptual data. Sensitivity of the correlation coefficient to several design parameters of this metric (such as number of critical-band filters, filter bandwidths, and aspects of the weighting function) will be discussed. [Work supported in part by an NSF grant.]
Calculations in apheresis (2014)

It's important to work smoothly with your apheresis equipment when you are an apheresis nurse. Attention should be paid to your donor/patient and the product you're collecting. It gives additional value to your work when you are able to calculate the efficiency of your procedures. You must be capable to obtain an optimal product without putting your donor/patient at risk. Not only the total blood volume (TBV) of the donor/patient plays an important role, but also specific blood values influence the apheresis procedure. Therefore, not all donors/patients should be addressed in the same way. Calculation of TBV, extracorporeal volume, and total plasma volume is needed. Many issues determine your procedure time. By knowing the collection efficiency (CE) of your apheresis machine, you can calculate the number of blood volumes to be processed to obtain specific results. You can calculate whether you need one procedure to obtain specific results or more. It's not always needed to process 3× the TBV. In this way, it can be avoided that the donor/patient is needless long connected to the apheresis device. By calculating the CE of each device, you can also compare the various devices for quality control reasons, but also nurses/operators.
Decolorization of Molasses Waste Water by a Thermophilic Strain,<i>Aspergillus fumigatus</i>G-2-6 Sadahiro (1987)

We screened for fungi that can decolorize molasses melanoidin in the tropical zone and isolated some strains, mainly in the genus Aspergillus. Of these, strain No. G-2–6 was most active and was identical with Aspergillus fumigatus based on detailed morphological studies. This strain decolorized about 75% of a molasses melanoidin solution when the strain was cultivated on a glycerol-peptone medium at 45°C for 3 days with shaking. In successive decolorization reusing the mycelia, this strain had more than 60% of the melanoidin-decolorizing activity at the eighth replacement in the presence of 4% glycerol. Continuous decolorization of molasses melanoidin solution in a jar fermentor had an almost constant decolorization yield of about 70% at a dilution rate of 0.014 hr-1. At the same time, about 51 % of the chemical oxygen demand and 56% of the total organic carbon in the initial solution were removed. In contrast, continuous decolorization of non-dialyzed molasses melanoidin solution removed a little more chemical oxygen demand and total organic carbon than those of dialyzed molasses melanoidin solution, but had a lower level of melanoidin-decolorizing activity (about 40%).
Intervención psicológica inmediata con víctimas de una catástrofe natural (1997)

En este articulo se describe la intervencion psicologica inmediata y sobre el terreno, con los familiares y las victimas de la riada que arraso el camping Nieves en Biescas (Huesca) el 7 de Agosto de 1996.
Concretamente se analiza:
- Las caracteristicas de las personas que acudian a la Pista de Hielo de Jaca para realizar las identificaciones de los cadaveres.
- El proceso de estructuracion familiar que posibilitaba el afrontamiento de esta tarea.
- El procedimiento de atencion seguido por los psicologos y los voluntarios.
- Cuestiones relativas al diseno ambiental del espacio en el que trabajaban.
- Los objetivos generales que orientaban la intervencion segun se tratase de personas que cumplian con los criterios diagnosticos de estres post-traumatico o los familiares que no habian sufrido la riada.
- Las tecnicas cognitivo-conductuales aplicadas a estos casos.
- El impacto emocional sobre el personal que realizaba tareas asistenciales.
Y, por ultimo, se ofrecen las razones que a juicio del autor, ponen de manifiesto la necesidad de contar con profesionales de la psicologia clinica en la respuesta institucional ante este tipo de catastrofe natural.
Reactive Oxygen Species, Cell growth, Cell Cycle Progression and Vascular Remodeling in Hypertension (2006)

Reactive oxygen species (ROS) include superoxide, hygrogen peroxide and hydroxyl radical. Under physiological conditions, all vascular cell types produce ROS in a controlled and regulated fashion, mainly through nonphagocyte NADPH oxidase. An imbalance between pro-oxidants and antioxidants results in oxidative stress. ROS are important intracellular signaling molecules. There is growing evidence that increased oxidative stress and associated oxidative damage are mediators of vascular injury in hypertension, as well as in other cardiovascular diseases. Oxidative stress causes vascular injury by reducing nitric oxide bioavailability, altering endothelial function and vascular contraction/dilation, promoting vascular smooth muscle cell proliferation and hypertrophy, and increasing extracellular matrix deposition and inflammation. The present review focuses on the regulatory role of ROS on cell growth and cell cycle progression and discusses implications of these events in vascular remodeling in hypertension.
Detection of apoptosis and changes in mitochondrial membrane potential with chloromethyl-X-rosamine (1999)

In a recent issue of Cytometry, the interpretation of flow cytometric data obtained after chloromethyl-X-rosamine (CMXRos) staining of apoptotic cells became the subject of a debate Ferlini et al. (1) analyzed data in an article by Macho et al. in Cytometry (2) and found discrepant changes in ''red'' fluorescence in experiments in which cells were stained with CMXRos alone and with a combination of DiOC 6 (3). They also noted that in fixed cells the retention of CMXRos depends on the amount of thiol level and that this may be a determinant of changes in CMXRos fluorescence. They concluded that further experimental work is needed to demonstrate the usefulness of CMXRos in measuring m .
A Reusable Platform for Building Sovereign Information Sharing Applications (2004)

Sovereign information sharing allows autonomous organizations to compute queries across databases in such a manner that nothing but the result is revealed. We present the design of a platform that can be used for building sovereign information sharing applications efficiently and quickly. In addition to the core protocols, the platform incorporates components for resource discovery, schema mapping, authentication, and hardwareassisted performance enhancement. We also describe a national security application we have built using this platform that shows the practicality of sovereign information sharing in virtual organizations.
Update on the Basic Helix-Loop-Helix Transcription Factor Gene Family in <i>Arabidopsis thaliana</i> (2003)

Basic helix-loop-helix (bHLH) transcription factors represent a family of proteins that contain a bHLH domain, a motif involved in binding DNA. Recently, two groups independently analyzed the BHLH gene family of Arabidopsis thaliana ([Heim et al., 2003][1]; [Toledo-Ortiz et al., 2003][2]). These
Game-Informed Learning: Applying Computer Game Processes to Higher Education (2005)

Computer games have made a significant cultural, social, economic, political, and technological impact on society (Newman 2004). Given the widespread popularity of video games, their ability to sustain long-term player engagement with challenging tasks (Gee 2003), and their tendency to elicit proactive player comm unities (Rheingold 1994), it should come as no great surprise that educators have become increasingly interested in the potential of such games as learning tools.
The Endocytic Recycling Protein EHD2 Interacts with Myoferlin to Regulate Myoblast Fusion (2008)

Skeletal muscle is a multinucleated syncytium that develops and is maintained by the fusion of myoblasts to the syncytium. Myoblast fusion involves the regulated coalescence of two apposed membranes. Myoferlin is a membrane-anchored, multiple C2 domain-containing protein that is highly expressed in fusing myoblasts and required for efficient myoblast fusion to myotubes. We found that myoferlin binds directly to the eps15 homology domain protein, EHD2. Members of the EHD family have been previously implicated in endocytosis as well as endocytic recycling, a process where membrane proteins internalized by endocytosis are returned to the plasma membrane. EHD2 binds directly to the second C2 domain of myoferlin, and EHD2 is reduced in myoferlin null myoblasts. In contrast to normal myoblasts, myoferlin null myoblasts accumulate labeled transferrin and have delayed recycling. Introduction of dominant negative EHD2 into myoblasts leads to the sequestration of myoferlin and inhibition of myoblast fusion. The interaction of myoferlin with EHD2 identifies molecular overlap between the endocytic recycling pathway and the machinery that regulates myoblast membrane fusion.