gebhart/patrepeval huggingface dataset:
An Electrospun Poly(vinylidene fluoride) Nanofibrous Membrane and Its Battery Applications (2003)

A polymer nanofibrous matrix suitable for polymer electrolytes in high‐performance lithium polymer batteries is presented. The poly(vinylidene fluoride) (PVdF) nanofibrous membrane (see Figure) was produced by electrospinning, which is briefly outlined. The characterization of the membrane—its porosity, network structure, mechanical strength, and electrochemical properties—is described.
Optical phased array radiating optical vortex with manipulated topological charges (2015)

Optical antennas are key elements in quantum optics emitting and sensing, and behave wide range applications in optical domain. However, integration of optical antenna radiating orbital angular momentum is still a challenge in nano-scale. We theoretically demonstrate a sub-wavelength phased optical antenna array, which manipulates the distribution of the orbital angular momentum in the near field. Orbital angular momentum with topological charge of 4 can be obtained by controlling the phase distribution of the fundamental mode orbital angular momentum in each antenna element. Our results indicate this phased array may be utilized in high integrated optical communication systems.
Release of biochemical markers of damage to neuronal and glial brain tissue is associated with short and long term neuropsychological outcome after traumatic brain injury (2001)

The analysis of post-traumatic release patterns of neurobiochemical markers of brain damage might help to identify patients with traumatic brain injury who run a risk of long term neuropsychological dysfunction.
Heparin-induced thrombocytopenia: an update (2005)

Heparin-induced thrombocytopenia (HIT) is the most important and most frequent drug-induced, immune-mediated type of thrombocytopenia. It is associated with significant morbidity and mortality if unrecognized. In this review, we briefly discuss the main features of heparin-induced thrombocytopenia, particularly analyzing the most recent advances in the pathophysiology, diagnosis and treatment of this syndrome.
Variational principles, surface evolution, PDEs, level set methods, and the stereo problem (1998)

We present a novel geometric approach for solving the stereo problem for an arbitrary number of images (= or >2). It is based upon the definition of a variational principle that must be satisfied by the surfaces of the objects in the scene and their images. The Euler-Lagrange equations that are deduced from the variational principle provide a set of partial differential equations (PDE's) that are used to deform an initial set of surfaces which then move toward the objects to be detected. The level set implementation of these PDE's potentially provides an efficient and robust way of achieving the surface evolution and to deal automatically with changes in the surface topology during the deformations, i.e., to deal with multiple objects. Results of an implementation of our theory also dealing with occlusion and visibility are presented on synthetic and real images.
Resource Allocation for CoMP With Multiuser MIMO-OFDMA (2011)

In this paper, we propose new resource allocation schemes with (or without) adaptive modulation for coordinated multipoint (CoMP) with multiuser multiple-input multiple-output orthogonal frequency-division multiple-access (MIMO-OFDMA). In the proposed schemes, a practical linear pre- and postprocessing technique is used to cancel interuser interference and decompose a single-user MIMO channel into parallel noninterfering spatial layers. The transmit power is allocated to spatial layers with (or without) adaptive modulation under the total base station (BS) power constraint and the per-BS power constraint. The set of users for each subcarrier is determined by the exhaustive search user allocation, the sum rate-based user allocation, and the random user allocation. Simulation results show that the proposed schemes enhance the sum rate.
Synthesis, purification and structural and functional characterization of recombinant form of a common genetic variant of human luteinizing hormone (2002)

A common genetic variant (V) of luteinizing hormone (LH), with two mutations (Trp(8)Arg and Ile(15)Thr) and an extra glycosylation consensus site (Asn(13)-Ala-Thr), is associated with abnormalities of reproductive function. To address the molecular basis of the functional differences between V- and wild-type (WT)-LH, recombinant (rec) forms of WT- and V-LH were synthesized in human embryonic kidney (HEK 293) cells. The rec hormones synthesized were rigorously purified employing affinity, immunoaffinity and ion exchange chromatographies (final purity approximately 12 000 IU/mg, 180-fold purification, 28% recovery). Functional properties of the hormone preparations were compared in vitro and in vivo. The molecular size of both rec LHs was 31 kDa, as determined by SDS-PAGE. Although the mutations in V-LHbeta did not significantly affect the affinity of LH receptor (LHR) binding (Kd approximately 0.4 nmol/L), V-LH had higher in vitro biopotency than WT-LH, in terms of mLTC-1 mouse Leydig tumor cell cAMP and progesterone (P) production, and steroidogenic acute regulatory protein (StAR) expression. In addition, in HEK 293 cells expressing the human LHR, V-LH demonstrated 1.8-fold higher response of inositol trisphosphate (IP(3)) production than WT-LH. Furthermore, HEK 293 cells expressing the ElK1 trans-reporting plasmids displayed 2.7-fold greater luciferase response to V-LH than WT-LH, documenting stimulation of the mitogen-activated protein kinase (MAPK) pathway. The in vivo half-life of V-LH was clearly faster (5-9 min) than that of WT-LH (12-22 min) and human chorionic gonadotropin (hCG; 50-70 min), when injected into rat circulation. It is worth noting that analysis by matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) demonstrated clear differences in structures of carbohydrate side chains attached to the two forms of rec LHs, including incomplete processing of high mannose glycans (Man(5,8,9)) in V-LH, suggesting different pathways in its intracellular trafficking. Collectively, the present findings provide the molecular basis for the qualitative and quantitative differences in LH action that are observed in carriers of the V-LHbeta allele.
Sol-gel PZT and Mn-doped PZT thin films for pyroelectric applications (2001)

Thin films of ferroelectric lead zirconate titanate (PbZr0.3Ti0.7O3 PZT30/70) and manganese-doped lead zirconate titanate ((Pb(Zr0.3Ti0.7)1-xMnx)O3, where x = 0.01, PM01ZT30/70; and x = 0.03, PM03ZT30/70) have been prepared using sol-gel processing techniques. These materials can be used as the pyroelectric thin films in uncooled infrared detectors. The thin films were prepared via a sol-gel route based on a hybrid solvent of methanol and ethanol with acetic acid, ethanolamine and ethylene glycol as additives. The final solution is non-moisture sensitive and stable. Films deposited on Pt/Ti/SiO2/Si substrates and annealed on a hot plate at 500-530 °C for a few minutes were seen to fully crystallize into the required perovskite phase and showed excellent ferroelectric behaviour, demonstrated by reproducible hysteresis loops (Pr = 33-37 µC cm-2, Ec( + ) = 70-100 kV cm-1, Ec(-) = -170 to -140 kV cm-1). The pyroelectric coefficient (p) was measured using the Byer-Roundy method. At 20 °C, p was 2.11×10-4 C m-2 K-1 for PZT30/70, 3.00×10-4 C m-2 K-1 for PM01ZT30/70 and 2.40×10-4 C m-2 K-1 for PM03ZT30/70 thin films. The detectivity figure-of-merit (FD) was 1.07×10-5 Pa-0.5 for PZT30/70, 3.07×10-5 Pa-0.5 for PM01ZT30/70 and 1.07×10-5 Pa-0.5 for PM03ZT30/70. These figures compare well with values reported previously.
TRAP Display: A High-Speed Selection Method for the Generation of Functional Polypeptides (2013)

Here, we describe a novel method that enables high-speed in vitro selection of functional peptides, peptidomimetics, and proteins via a simple procedure. We first developed a new cell-free translation system, the TRAP system (transcription-translation coupled with association of puromycin linker), which automatically produces a polypeptide library through a series of sequential reactions: transcription, association of puromycin-DNA linker, translation, and conjugation between the nascent polypeptide and puromycin-DNA linker. We then applied the TRAP system for the selection of macrocyclic peptides against human serum albumin. Six rounds of selection using TRAP display were performed in approximately 14 h, yielding macrocyclic peptides with nanomolar affinity to their target protein. Because TRAP display enables high-speed selection of functional polypeptides, it will facilitate the generation of various polypeptides that are useful for biological and therapeutic applications.
GeoEcho: Inferring User Interests from Geotag Reports in Network Traffic (2014)

Being transmitted as part of numerous Internet services, geo location data is increasingly bringing hints of people's real-world activities into Internet traffic. This paper focuses on the discovery of key properties that motivate personal activities - locational interests. We propose and design GeoEcho, a mobile traffic analysis system that extracts and analyses a wealth of latitude-longitude geotag reports with the purpose of identifying the points of interest (PoI) which people actually visit. The key challenge in such identification is that geotag reports are commonly sent arbitrarily, sparsely and without a sufficient accuracy to uniquely identify any PoI. In our analysis of a two-week trace from a large North-American cell phone operator, we show that 22% of geo reports do not even represent actual people's positions, while another 45% of the reports have low accuracy, such that they ambiguously indicate a number of potential PoIs. We devise methods that effectively identify and prune irrelevant geo information and infer personal interests of individuals. Thereby creating representative profiles of personal interests, our key results reveal that users show interest in a limited number of topics, and their interests are largely unique and stable over time. Our analysis shows a significant GeoEcho usability in various contexts ranging from generic user profile and user group analysis, to advertising and security applications.
3-D Rat Brain Phantom for High-Resolution Molecular Imaging (2009)

With the steadily improving resolution of novel small-animal single photon emission computed tomography (SPECT) and positron emission tomography devices, highly detailed phantoms are required for testing and optimizing these systems. We present a three-dimensional (3-D) digital and physical phantom pair to represent, e.g., cerebral blood flow, glucose metabolism, or neuroreceptor binding in small regions of the rat brain. The anatomical structures are based on digital photographs of the uncut part of a rat brain cryosection block. The photographs have been segmented into ventricles and gray and white matter and have been stacked afterwards. In the resulting voxelized digital phantom, tracer concentration in gray and white matter can be scaled independently. This is of relevance since, e.g., cerebral blood flow or metabolism are much higher in gray than in white matter. The physical phantom is based on the digital phantom and has been manufactured out of hardened polymer using rapid prototyping, a process in which complicated 3-D objects can be built up layer by layer. X-ray computed tomography and high-resolution SPECT images of the physical phantom are compared with the digital phantom. The detailed physical phantom can be filled bubble-free. Excellent correspondence is shown between details in the digital and physical phantom. Therefore, this newly developed brain phantom will enable the optimization of high-resolution imaging for recovery of complex shaped molecular distributions.
Image segmentation and registration for the analysis of joint motion from 3D MRI (2006)

We report an image segmentation and registration method for studying joint morphology and kinematics from <i>in vivo</i> MRI scans and its application to the analysis of ankle joint motion. Using an MR-compatible loading device, a foot was scanned in a single neutral and seven dynamic positions including maximal flexion, rotation and inversion/eversion. A segmentation method combining graph cuts and level sets was developed which allows a user to interactively delineate 14 bones in the neutral position volume in less than 30 minutes total, including less than 10 minutes of user interaction. In the subsequent registration step, a separate rigid body transformation for each bone is obtained by registering the neutral position dataset to each of the dynamic ones, which produces an accurate description of the motion between them. We have processed six datasets, including 3 normal and 3 pathological feet. For validation our results were compared with those obtained from 3DViewnix, a semi-automatic segmentation program, and achieved good agreement in volume overlap ratios (mean: 91.57%, standard deviation: 3.58%) for all bones. Our tool requires only 1/50 and 1/150 of the user interaction time required by 3DViewnix and NIH Image Plus, respectively, an improvement that has the potential to make joint motion analysis from MRI practical in research and clinical applications.
Imprinted Gene Dosage Is Critical for the Transition to Independent Life (2012)

Neonatal survival in mammals is crucially dependent upon maintenance of body temperature. Neonatal body temperature is largely maintained by thermogenesis in brown adipose tissue (BAT). BAT develops perinatally in mice requiring integration of adipogenic and thermoregulatory gene pathways. We describe a regulatory mutation in the imprinted gene cluster on mouse chromosome 12 resulting in early postnatal lethality. Maternal inheritance of this mutation impairs the ability of young mice to maintain body temperature. While mechanisms of perinatal BAT development are well understood, our work highlights a second phase of BAT recruitment necessary to support small animals newly independent of the nest. We show that the imprinted delta-like homolog 1/preadipocyte factor (Dlk1/Pref1) and iodothyronine deiodinase type 3 (Dio3) functions converge on the development of brown fat at the transition to independent life. This shows that appropriate dosage control at imprinted loci can act as a critical determinant in postnatal survival during phases of physiological adaptation.
Sputtering Highly C-Axis-Oriented AlN Films on Y-128° LiNbO<sub>3</sub> (2000)

Highly c -axis-oriented aluminum nitride (AlN) films were successfully prepared, without heating the substrate, on Y-128° LiNbO 3 substrate by rf magnetron sputtering. The dependence of the sputtering pressure and the physical structures of the films (crystalline structure and micromorphology) were investigated. The results showed that the highly c -axis-oriented AlN films appeared at the lower sputtering pressures and the films with the best orientation and microstructure were prepared at 3 m Torr. The deposition rate also increased as the sputtering pressure decreased.
Zebrafish as a “Biosensor”? Effects of Ionizing Radiation and Amifostine on Embryonic Viability and Development (2006)

The zebrafish (Danio rerio) has emerged as a popular vertebrate model system for cancer and treatment-related research. Benefits include ease of care, rapid development, optical clarity of embryos, which allows visualization of major organ systems, and opportunities for genetic manipulation. However, specific parameters of radiation sensitivity have not been systematically documented. We investigated the effects of radiation and a radiomodifier on zebrafish viability and embryonic development. Embryos were exposed to gamma-radiation (5, 10, or 20 Gy) at sequential times postfertilization and serially assessed for viability and morphologic abnormalities. As expected, lethality and morphologic perturbations were more pronounced earlier in embryogenesis and with higher radiation doses and were partially reversed by amifostine. The effects of radiation and concurrent treatment with amifostine on the developmental organization of the eye and brain were striking. Radiation resulted in hypocellularity and disorganization of the cellular layers of the retina, effects partially reversed by amifostine, as well as lens opacification. Radiation strikingly reduced the volume of brain, but the volume loss was substantially blocked by amifostine. Increased terminal deoxyribonucleotidyl transferase-mediated dUTP nick end labeling signal was noted in both the irradiated eye and brain, but reduced by amifostine. Finally, irradiating embryos resulted in caspase activation detectable in 96-well microplates, which was proportional to the number of embryos and radiation dose; the degree of activation was markedly reduced by amifostine. These results together suggest the power and versatility of the zebrafish in assessing the effects of radiation and radiomodifiers on organ and tissue development.
Visual servoed micropositioning for protein manipulation tasks (2003)

In this paper, we present a framework for cell manipulation tasks with visual servoing micromanipulation strategies. A vision based micropositioner is designed in order to address the requirement of high precision needed to perform manipulation of objects under 100 /spl mu/m in size. The system calibration (microscope-camera-micropositioner) and the model of the observed scene are not known. Experimental results for micropositioning tasks with respect to protein cells are presented and demonstrate the validity of the proposed approach.
Protein crystal structure solution by fast incorporation of negatively and positively charged anomalous scatterers (2001)

The preparation of derivatives by the traditional methods of soaking is one of the most time-consuming steps in protein crystal structure solution by X-ray diffraction techniques. The 'quick cryosoaking' procedure for derivatization with halides (monovalent anions) offers the possibility of significantly speeding up this process [Dauter et al. (2000), Acta Cryst. D56, 232-237]. In the present work, an extension of this technique is proposed and the use of two different classes of compounds (monovalent and polyvalent cations) that can be successfully utilized in the quick cryosoaking procedure for the derivatization and phasing of protein crystals is described. This approach has been tested on hen egg-white lysozyme and has been successfully used to solve the structure of a novel trypsin inhibitor. The possibility of using cations in the fast cryosoaking procedure gives additional flexibility in the process of derivatization and increases the chances of success in phase determination. This method can be applied to high-throughput crystallographic projects.
Analysis of Factors Influencing Consumers' Purchase Intention Based on Perceived Value in E-commerce Clothing Pre-sale Model (2020)

The e-commerce pre-sale model for apparel is a kind of marketing method for retailers to generate actual consumption through online consumers' payment of deposits or full payments. The online apparel's re-sale model, an effective competition, can effectively reduce inventory, predict production and increase the actual profits of enterprises. Based on the perceived value of consumers, SPSS21.0 and AMOS21.0 are used to analyze the collected information, verify the hypotheses of the initial model of consumers' purchase intention under the pre-sale model, and then make modifications. The relationship among variables can be revealed and this paper explains the performance of the influence of pre-sale mode on consumers' purchase intention, by analyzing correlations among perceived benefit, perceived sacrifice and perceived value, as well as the impact on purchase intention.
The endothelium in acute lung injury/acute respiratory distress syndrome (2008)

Mechanistic studies have delivered several interventions, which are effective in preventing and treating experimental acute lung injury and have thus provided objectives for translational studies. Some of these modalities may evolve into clinically useful tools in the treatment of this devastating illness.
Aerial manipulator with perching and door-opening capability (2015)

This paper presents an aerial robot with a manipulator to implement door opening mission. Although general aerial robots have advantages of flying in the three-dimensional space freely, they don't have any capability of moving to another room when the door is closed. To overcome this problem, we propose a new configuration of an aerial manipulator with perching function, knob-twisting function, and door-pushing function. Perching function can be achieved by integration of door-approach control, the mechanism for perching and attitude-change control. With regard to knob-twisting function, the design concept of a light-weight manipulator generating large enough force to twist the knob is introduced, which is composed of a soft-bag actuator with variable restriction to perform the arbitrary curved trajectory. On the other hand, the door pushing force is aimed to be generated by the lift of the propeller, which is helpful to avoid gaining the additional weight. The validity of the proposed methods is experimentally verified by using the developed prototype.
The Binding of Cu(II) and Zn(II) Ions by Heparin (1982)

Abstract A method is described for measuring the ion binding capacity of heparin. The method is based on the separation of the zinc or copper complex of heparin by gel permeation. It was found that the number of the cation per average molecule of heparin depends on the pH and on the initial concentration of metal cation in solution. The absorption spectrum of heparin-Cu complex is described. The molar absorptivity of this complex is determined, as is the complex formation constant.
The Human T Cell Receptor: Analysis with Cytotoxic T Cell Clones (1983)

Employing human T lymphocyte clones and monoclonal antibodies to their surface glycoproteins, the antigen receptors on these cells have been defined. Based on functional and biochemical data, it is shown that each T cell displays two major recognition units on its surface. One structure consists of the antigen-binding region (Tin-T3) which views antigen X in the context of a polymorphic region of an MHC molecule. A second (T8 or T4) serves as an associative recognition structure for a constant region of the class I or class II molecule. The antigen-binding structure is a heterodimer of disulfide linked 49KD and 43KD subunits, which contains clonally unique variable regions. These are non-covalently associated with the 20/25KD monomorphic T3 molecule expressed on all mature human T lymphocytes. The associative-recognition element on an individual clone is either T8 or T4, depending on its subset derivation. It is likely that these glycoproteins bind to constant regions of class I or class II molecules, respectively, and are independent of the Tin-T3 complex complex.