gebhart/patrepeval huggingface dataset:
The Phosphoenolpyruvate-Dependent Phosphotransferase System of Staphylococcus aureus. 1. Amino-Acid Sequence of the Phosphocarrier Protein HPr (1977)

The primary structure of the histidine-containing phosphocarrier protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system from Staphylococcus aureus was determined by automated Edman degradation. The complete sequence was deduced from the direct analysis of the protein by automated Edman degradation in a liquid-phase sequencer of Edman and from the sequence of tryptic, thermolytic and cyanogen bromide peptides as obtained by automated Edman degradation in a solid-phase sequencer of Laursen. The amino-acid sequence was found to be Met-Glu-Gln-Asn-Ser-Tyr-Val-Ile-Ile-Asp-Glu-Thr-Gly-Ile-His-Ala-Arg-Pro-Ala-Thr-Met-Leu-Val-Gln-Thr-Ala-Ser-Lys-Phe-Asp-Ser-Ile-Asp-Gln-Gly-Gly-Tyr-Asp-Ser-Met-Gln-Leu-Lys-Ser-Leu-Gly-Val-Gly-Lys-Asp-Glu-Glu-Ile-Thr-Ile-Tm-Ser-Ala-Asp-Lys-Lys-Glu-Gly-Leu-Thr-Lys-Met-Ser-Ile-Val. The 70 residues correspond to a molecular weight of 7685. The one histidine involved in the phosphotransfer reaction of this protein was found at position 15 as part of a region of the sequence which has no predictable secondary structure. It is suggested that this protein belongs to the group of male proteins with the active center located on a protrusion rather than a cleft.
An Authentication Scheme in Peer-to-Peer Based Live Stream Sharing Management Systems (2008)

Peer-to-peer based live stream sharing platform is a novel technique for distributing visual data. Because of developments in computer equipment and network service, viewers can watch shows via the Internet rather than through the conventional broadcasted manner. Although data access via the Internet is more convenient, it is more difficult to manage the data transmission, and issues such as copyrighting become more of a problem. In this paper, we will propose an authentication scheme which accounts for this peer-to-peer based structure and tolerates a large amount of participants.
An experimental adaptive echo canceller for long distance telephone circuits (1973)

A new concept of eliminating spurious echoes in long distance telephone circuits by adaptive echo cancellation has been experimentally implemented. In this paper the principles underlying the experimental device and the techniques used in their implementation are presented. Experimental results obtained from measurements in the laboratory as well as in a practical telephone system environment are reported.
Construction of Elderly Mutual Aid Time Bank Based on Blockchain (2019)

With the increase of the aging population, the old-age care industry faces new challenges. Mutual support for the elderly as a new model of old-age care has attracted much attention. Although Time Banks have provided a mutual assistance pension solution, still, there are some problems. This paper combines the blockchain with the time bank to build a blockchain time bank, and solves the problems faced by the current time banks. At the same time, the article innovates the mutual help pension model and broadens the user base and participating institutions, which makes the pension system more complete and rich.
Modulation of the Endothelial Nitric-oxide Synthase-Caveolin Interaction in Cardiac Myocytes (1998)

The endothelial isoform of nitric oxide synthase (eNOS) is dually acylated and thereby targeted to signal-transducing microdomains termed caveolae. In endothelial cells, eNOS interacts with caveolin-1, which represses eNOS enzyme activity. In cardiac myocytes, eNOS associates with the muscle-specific caveolin-3 isoform, but whether this interaction affects NO production and regulates myocyte function is unknown. We isolated neonatal cardiac myocytes from mutant mice with targeted disruption of the eNOS gene and transfected them with wild-type (WT) eNOS or myristoylation-deficient (myr-) eNOS mutant cDNA. In myocytes expressing WT eNOS, the muscarinic cholinergic agonist carbachol completely abrogated the spontaneous beating rate and induced a 4-fold elevation of the cGMP level. By contrast, in the myr- eNOS myocytes, carbachol failed to exert its negative chronotropic effect and to increase cGMP levels. We then used a reversible permeabilization protocol to load intact neonatal rat myocytes with an oligopeptide corresponding to the caveolin-3 scaffolding domain. This peptide completely and specifically inhibited the carbachol-induced negative chronotropic effect and the accompanying cGMP elevation. Thus, our results suggest that acylated eNOS may couple muscarinic receptor activation to heart rate control and indicate a key role for eNOS/caveolin interactions in the cholinergic modulation of cardiac myocyte function.
Ferrocenyldimethyl-[2.2]-cryptand: solid state structure of the external hydrate and alkali and alkaline-earth-dependent electrochemical behaviour (1991)

1,1′-(1,4,10,13-Tetraoxa-7,16-diazacyclooctadecane-7,16-diyldimethyl)ferrocene, ferrocenyldimethyl-[2.2]-cryptand 1, binds water on its external face rather than internally and exhibits electrochemical behaviour that is strongly dependent on alkali and alkaline earth metal cations.
Overproduction of Nitric Oxide in Cytokine-Mediated and Septic Shock (1992)

bution to the apparent aggressiveness of breast tumors. Biological studies of a limited number of p53 mutations suggest, for instance, that some p53 mutations are more "oncogenic" than others (6). Similarly, p53 genes having mutations in exons 5 and 6 encode proteins which bind heat shock protein (hsp) 70, whereas exons 7 and 8 mutant proteins do not (14). In this regard, Davidoff et al. (14) have found an association between breast tumors having mutations in exons 5 and 6 and those patients who show a humoral response to p53. In contrast, all of the patients who had a p53-positive tumor, but who were p53 antibody negative, had the mutation in exon 7 or 8. Based on these and other studies, one future direction should be to determine the prognostic significance of p53 mutations as a function of the affected exon or domain.
Evaluation of Antimicrobial and Lipopolysaccharide-Neutralizing Effects of a Synthetic CAP18 Fragment against<i>Pseudomonas aeruginosa</i>in a Mouse Model (1998)

ABSTRACT CAP18 (cationic antimicrobial protein; 18 kDa) is a neutrophil-derived protein that can bind to and inhibit various activities of lipopolysaccharide (LPS). The 37 C-terminal amino acids of CAP18 make up the LPS-binding domain. A truncated 32-amino-acid C-terminal fragment of CAP18 had potent activity against Pseudomonas aeruginosa in vitro. We studied the antimicrobial and LPS-neutralizing effects of this synthetic truncated CAP18 peptide (CAP18 106–137 ) on lung injury in mice infected with cytotoxic P. aeruginosa . To determine its maximal effect, the CAP18 106–137 peptide was mixed with bacteria just prior to tracheal instillation, and lung injury was evaluated by determining the amount of leakage of an alveolar protein tracer ( 125 I-albumin) into the circulation and by the quantification of lung edema. The lung injury caused by the instillation of 5 × 10 5 CFU of P. aeruginosa was significantly reduced by the concomitant instillation of CAP18 106–137 . However, the administration of CAP18 106–137 alone, without bacteria, induced lung edema, suggesting that it has some toxicity. Also, the peptide did not significantly reduce the number of bacteria that had been simultaneously instilled, nor did it significantly improve the survival of the infected mice. The addition of CAP18 106–137 to aztreonam along with the bacteria did decrease the level of antibiotic-induced release of inflammatory mediators including tumor necrosis factor alpha, interleukin-6, and nitric oxide and also improved the survival of the mice. Therefore, more investigations are needed to confirm the toxicities and the therapeutic benefits of CAP18 106–137 as an adjunctive therapy to antibiotics in the treatment of infections caused by gram-negative bacteria.
131I–anti-CD45 antibody plus busulfan and cyclophosphamide before allogeneic hematopoietic cell transplantation for treatment of acute myeloid leukemia in first remission (2005)

In an attempt to improve outcomes for patients with acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (HCT), we conducted a phase 1/2 study in which targeted irradiation delivered by 131I-anti-CD45 antibody was combined with targeted busulfan (BU; area-under-curve, 600-900 ng/mL) and cyclophosphamide (CY; 120 mg/kg). Fifty-two (88%) of 59 patients receiving a trace 131I-labeled dose of 0.5 mg/kg anti-CD45 murine antibody had higher estimated absorbed radiation in bone marrow and spleen than in any other organ. Forty-six patients were treated with 102 to 298 mCi (3774-11 026 MBq) 131I, delivering an estimated 5.3 to 19 (mean, 11.3) Gy to marrow, 17-72 (mean, 29.7) Gy to spleen, and 3.5 Gy (n = 4) to 5.25 Gy (n = 42) to the liver. The estimated 3-year nonrelapse mortality and disease-free survival (DFS) were 21% and 61%, respectively. These results were compared with those from 509 similar International Bone Marrow Transplant Registry patients who underwent transplantation using BU/CY alone. After adjusting for differences in age and cytogenetics risk, the hazard of mortality among all antibody-treated patients was 0.65 times that of the Registry patients (95% CI 0.39-1.08; P = .09). The addition of targeted hematopoietic irradiation to conventional BU/CY is feasible and well tolerated, and phase 2 results are sufficiently encouraging to warrant further study.
Current trends in computer science graduate admissions (1999)

This article addresses some questions students have when completing applications to maximize their chances for admission to graduate school. We conducted a survey of the admissions process among the top computer science programs. Our study shows how admission committees weigh different sections of an application and overall trends of admission. The results are of interest to both applicants and computer science graduate faculty.
Synthesis of glycolipids as membrane‐bound stabilizing carbohydrates (1995)

Abstract Stereoselective synthesis of glycosides (glucosides, galactosides, mannosides, and xylosides) is reported using long‐chain alcohols with 0–4 ethoxy spacers as potential anchored cryoprotectors.
The keepup recommender system (2007)

In this short paper, we describe our RSS recommender system, KeepUP. Too often recommender systems are seen as black box systems, resulting in general perplexity and dissatisfaction from users who are treated as passive, isolated consumers. Recent literature observes that recommendations rarely occur within such isolation and that there may be potential within more socially-orientated approaches. With KeepUP, we outline the design of a recommendation process that is based on an implicit social network where the relevancy and meaning of information can be negotiated not only with the recommender system but also with other users. Our overall goal is to support the formation and development of online communities of interest.
The Grid: A New Infrastructure for 21st Century Science (2002)

As computer networks become cheaper and more powerful, a new computing paradigm is poised to transform the practice of science and engineering.
Immunotherapy for Glioblastoma: Adoptive T-cell Strategies (2018)

Glioblastoma (GBM) is a devastating disease with an extremely poor prognosis. Immunotherapy via adoptive cell transfer (ACT), especially with T cells engineered to express chimeric antigen receptors (CAR), represents a particularly promising approach. Despite the recent success of CAR T cells for blood cancers, the question remains whether this powerful anticancer therapy will ultimately work for brain tumors, and whether the primary immunologic challenges in this disease, which include antigenic heterogeneity, immune suppression, and T-cell exhaustion, can be adequately addressed. Here, we contextualize these concepts by reviewing recent developments in ACT for GBM, with a special focus on pioneering clinical trials of CAR T-cell therapy.
The complex structure of calmodulin bound to a calcineurin peptide (2008)

Abstract The activity of the protein phosphatase calcineurin (CN) is regulated by an autoinhibition mechanism wherein several domains from its catalytic A subunit, including the calmodulin binding domain (CaMBD), block access to its active site. Upon binding of Ca 2+ and calmodulin (Ca 2+ /CaM) to CaMBD, the autoinhibitory domains dissociate from the catalytic groove, thus activating the enzyme. To date, the structure of the CN/CaM/Ca 2+ complex has not been determined in its entirety. Previously, we determined the structure of a fusion protein consisting of CaM and a 25‐residue peptide taken from the CaMBD, joined by a 5‐glycine linker. This structure revealed a novel CaM binding motif. However, the presence of the extraneous glycine linker cast doubt on the authenticity of this structure as an accurate representation of CN/CaM binding in vivo . Thus, here, we have determined the crystal structure of CaM complexed with the 25‐residue CaMBD peptide without the glycine linker at a resolution of 2.1 Å. The structure is essentially identical to the fusion construction which displays CaM bound to the CaMBD peptide as a dimer with an open, elongated conformation. The N‐lobe from one molecule and C‐lobe from another encompass and bind the CaMBD peptide. Thus, it validates the existence of this novel CaM binding motif. Our experiments suggest that the dimeric CaM/CaMBD complex exists in solution, which is unambiguously validated using a carefully‐designed CaM‐sepharose pull‐down experiment. We discuss structural features that produce this novel binding motif, including the role of the CaMBD peptide residues Arg‐408, Val‐409, and Phe‐410, which work to provide rigidity to the otherwise flexible central CaM helix joining the N‐ and C‐lobes, ultimately keeping these lobes apart and forcing “head‐to‐tail” dimerization to attain the requisite N‐ and C‐lobe pairing for CaMBD binding. Proteins 2008. © 2008 Wiley‐Liss, Inc.
Effect of Microstructure and Sintering Temperature on Fabrication of NITE-SiC<sub>f</sub>/SiC Composite (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Central nervous system depressants. <b>15</b>. 1,4‐benzodiazepines (1979)

Abstract A number of 5‐phenyl‐1,4‐benzodiazepines and a few 2‐phenyl‐1,4‐benzodiazepines were prepared and screened for central nervous system activity in mice. Some were highly active. An unusual substitution of 7‐chloro‐1,3‐dihydro‐5‐phenyl‐2 H ‐1,4‐benzodiazepin‐2‐one by trichloro‐acetylisocyante is reported.
Combining Biometric Fractal Pattern and Particle Swarm Optimization‐Based Classifier for Fingerprint Recognition (2010)

This paper proposes combining the biometric fractal pattern and particle swarm optimization (PSO)‐based classifier for fingerprint recognition. Fingerprints have arch, loop, whorl, and accidental morphologies, and embed singular points, resulting in the establishment of fingerprint individuality. An automatic fingerprint identification system consists of two stages: digital image processing (DIP) and pattern recognition. DIP is used to convert to binary images, refine out noise, and locate the reference point. For binary images, Katz′s algorithm is employed to estimate the fractal dimension (FD) from a two‐dimensional (2D) image. Biometric features are extracted as fractal patterns using different FDs. Probabilistic neural network (PNN) as a classifier performs to compare the fractal patterns among the small‐scale database. A PSO algorithm is used to tune the optimal parameters and heighten the accuracy. For 30 subjects in the laboratory, the proposed classifier demonstrates greater efficiency and higher accuracy in fingerprint recognition.
A Monolithic High-Efficiency 2.4-GHz 20-dBm SiGe BiCMOS Envelope-Tracking OFDM Power Amplifier (2007)

A monolithic SiGe BiCMOS envelope-tracking power amplifier (PA) is demonstrated for 802.11g OFDM applications at 2.4 GHz. The 4-mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> die includes a high-efficiency high-precision envelope amplifier and a two-stage SiGe HBT PA for RF amplification. Off-chip digital predistortion is employed to improve EVM performance. The two-stage amplifier exhibits 12-dB gain, <5% EVM, 20-dBm OFDM output power, and an overall efficiency (including the envelope amplifier) of 28%.
Amyloidosis and female protein in the Syrian hamster. Concurrent regulation by sex hormones. (1990)

Previous results have shown that when compared to male Syrian hamsters, female Syrian hamsters have a distinct predisposition to acquire amyloidosis either normally with aging or experimentally with sodium caseinate or diethylstilbestrol (DES) treatments. In the present study, we tested the influence of testosterone on expression of amyloid to determine if this hormone was solely responsible for the sex-limited amyloidosis of the Syrian hamster. Males deprived of testosterone by castration acquired amyloid at an unusually young age, an age of onset similar to that in female hamsters. Also, the amyloidogenic effect of DES in male Syrian hamsters was inhibited by concomitant injections of testosterone, indicating that estrogens induce amyloid in male hamsters by inhibiting testosterone synthesis. When administered to female hamsters, testosterone inhibited expression of amyloid in aging female Syrian hamsters and extended the life span of this gender. Of the two components of amyloid, the major component Amyloid A-derived fibril or the minor constituent, Amyloid P component, only the P component is under sex hormone control in the Syrian hamster; testosterone inhibits the hepatic synthesis of the P component homologue (called female protein), which is normally expressed 100-200-fold greater in female vs. male Syrian hamster. In general, the serum level of female protein under various experimental conditions correlated with the presence of amyloid and indicated that in the Syrian hamster the P component homologue is of primary importance in the deposition of amyloid.
Recent airborne radar observations of migrant pests in the United States (1990)

Abstract An airborne radar was used to detect insets dispersing from an area of about 200 000 hectares of maize infested with Heliothis zea (Boddie) and Spodoptera frugiperda (J. E. Smith), in the lower Rio Grande River Valley (LRGRV) of northeast Mexico. During the night of 20 June 1989, a ground-based radar, located at the downwind edge of the maize production area, detected early evening take-off and departure of flying insects. Simultaneously, an airborne radar detected low numbers of airborne insects 50 km downwind. Large numbers of flying insects were detected 200-700 m above ground as the aircraft approached the source. The edge of the emigrating cloud of insects was clearly marked by a rapid change in numbers of airborne insects; the cloud was subsequently intercepted at various locations downwind of the source area. The downwind edge of the insect cloud appeared to travel northward at least 400 km in 7.7 h. Maximum H. zea moth emergence occurred on 18 June from maize in the LRGRV. Three days later, maximum oviposition occurred in cotton near Uvalde, 370 km from the LRGRV and downwind on the night of the mass migration observed.
Structure and development of rabbit pepsinogens. Stage-specific zymogens, nucleotide sequences of cDNAs, molecular evolution, and gene expression during development. (1990)

In order to clarify the structure and development of rabbit pepsinogens, purification and molecular cloning of these proteins were performed at various developmental stages. Several pepsinogens were isolated, and they were classified as pepsinogens F and M, and into pepsinogen groups I, II, and III. The relative levels and specific activities of the various pepsinogens changed significantly during development. Pepsinogens F and M were present only at the early postnatal stage, and their level was higher than those of other pepsinogens at this stage. Pepsinogens in groups I, II, and III were the predominant zymogens at the late postnatal stage. cDNA clones encoding all of these pepsinogens were obtained, with the exception of pepsinogens I and M, and the nucleotide sequences were determined. Each cDNA contained a leader region (signal peptide), a pro-region (activation segment), and a pepsin region, of 15, 44, and 328 residues, respectively, with the exception of the cDNA for pepsinogen F in which the pro- and pepsin regions were composed of 43 and 330 residues, respectively. Pepsinogens in groups II and III exhibited a high degree of similarity with one another, whereas many substitutions were found in pepsinogen F. A unique substitution in the activation segment of pepsinogen F, namely, Gly----Asp at position 21, was found, which made the structural features of this segment more specific. A phylogenic tree was constructed from the differences in nucleotide sequences and showed clearly that each pepsinogen in groups II and III could be classified as pepsinogen A, a major pepsinogen in mammals. Pepsinogen F diverged significantly from these groups and may be a new type of pepsinogen. Northern analysis revealed that the expression of the gene for pepsinogen F was restricted to the early postnatal stage, and the expression of genes for pepsinogens in groups II and III was detected predominantly at later stages, a result that shows the switching of gene expression from fetal pepsinogen to adult pepsinogens during development.
Isolation of rabbit single domain antibodies to B7-H3 via protein immunization and phage display (2020)

Single domain antibodies have certain advantages including their small size, high stability and excellent tissue penetration, making them attractive drug candidates. Rabbit antibodies can recognize diverse epitopes, including those that are poorly immunogenic in mice and humans. In the present study, we established a method to isolate rabbit VH single domain antibodies for potential cancer therapy. We immunized rabbits with recombinant human B7-H3 (CD276) protein, made a phage-displayed rabbit VH single domain library with a diversity of 7 × 10<sup>9</sup>, and isolated two binders (A1 and B1; also called RFA1 and RFB1) from phage panning. Both rabbit VH single domains exhibited antigen-dependent binding to B7-H3-positive tumor cell lines but not B7-H3 knockout tumor cell lines. Our study shows that protein immunization followed by phage display screening can be used to isolate rabbit single domain antibodies. The two single domain antibodies reported here may have potential applications in cancer immunotherapy.
Fabrication and experimental observation of monolithic multi-air-core fiber array for image transmission (2008)

Two kinds of multi-air-core optical fibers were designed and fabricated by extruding optical grade PMMA pellets and drawing to fiber. The imaging function of the fibers was investigated with home-made specialized microscopy. This new type of fiber provides strong potential for applications in endoscopy, chemical sensing, biosensors, fiber-optical faceplates.