gebhart/patrepeval huggingface dataset:
An efficient measurement report mechanism for Long Term Evolution networks (2011)

LONG-TERM EVOLUTION (LTE) is expected to ensure 3GPP's competitive edge over other cellular technologies. The advantages of LTE include improving end-user throughputs, and reducing latency. LTE systems utilize a network-controlled and the user equipment (UE) assisted handover procedure for mobility in connected mode. In this paper, we first introduce the measurement report mechanism defined in the specification of LTE systems. We further explore possible problems and the impact on handover decision. Followed, we propose rules for timer adjustment, including timeToTrigger and reportInterval. Our mechanism aims at reducing incorrect handover decisions and maximizing the throughput performance. By conducting simulation, we evaluate the designed mechanism. The simulation results show that our proposed mechanism does achieve our design goals.
The expression of nicotinic acetylcholine receptors by PC12 cells treated with NGF (1992)

The expression of neuronal nicotinic ACh receptors (nAChRs) and the subunits that compose these receptors by PC12 cells exposed to NGF has been studied. The analysis of total RNA reveals that the neuronal nAChR subunits alpha 3, alpha S, beta 2, beta 3, and beta 4, but not alpha 2 and alpha 4, are expressed in our PC12 cells. Within 48 hr of adding NGF to cultures, the RNA corresponding to alpha 3, alpha 5, beta 3, and beta 4 is decreased, but the beta 2 RNA increases for up to 6 d after NGF treatment. To determine the influence of NGF treatment on subunit protein expression, subunit-specific antisera were prepared. Immunocytochemistry detected antigen for alpha 3, alpha 5, beta 2, beta 3, and beta 4 (but not alpha 2 and alpha 4) in both NGF-treated and nontreated PC12 cells. The expression of nAChR subunit proteins, as measured by direct binding of antibodies to PC12 cells, does not change subsequent to 6 d of treatment with NGF. Whole-cell recording of PC12 cells shows that both the individual cell current density and response to the agonist cytisine were not altered after 5-7 d in NGF. However, the number of cells exhibiting detectable ACh-induced currents doubled. These results indicate that NGF increases the number of PC12 cells expressing ACh-sensitive nAChR currents but the activation is not the result of altering the amounts of individual nAChR subunit proteins. These data, taken together with the decrease in most nAChR subunit RNAs (except beta 2), suggest that NGF regulation of nAChRs may be through a posttranscriptional mechanism.
Conductive metal–organic frameworks and networks: fact or fantasy? (2012)

Electrical conduction is well understood in materials formed from inorganic or organic building blocks, but their combination to produce conductive hybrid frameworks and networks is an emerging and rapidly developing field of research. Self-assembling organic-inorganic compounds offer immense potential for functionalising material properties for a wide scope of applications including solar cells, light emitters, gas sensors and bipolar transparent conductors. The flexibility of combining two distinct material classes into a single solid-state system provides an almost infinite number of chemical and structural possibilities; however, there is currently no systematic approach established for designing new compositions and configurations with targeted electronic or optical properties. We review the current status in the field, in particular, the range of hybrid systems reported to date and the important role of materials modelling in the field. From theoretical arguments, the Mott insulator-to-metal transition should be possible in semiconducting metal-organic frameworks, but has yet to be observed. The question remains as to whether electro-active hybrid materials will evolve from chemical curiosities towards practical applications in the near term.
Cell Biology, A Short Course (2003)

"This text tells the story of cells as the units of life in a colorful and student-friendly manner, taking an "essentials only" approach. By using the successful model of previously published "Short Courses," this text succeeds in conveying the key points without overburdening the reader with secondary information. The authors skillfully present concepts by illustrating them with clear diagrams and examples from current research. Special boxed sections focus on the importance of cell biology in medicine and industry today. This text is completely updated from the successful "Cell Biology, A Short Course, 3e," includes new chapters"--
The core oligosaccharide component from <i>Mannheimia (Pasteurella) haemolytica</i> serotype Al lipopolysaccharide contains <scp>L</scp>-<i>glycero</i>-<scp>D</scp>-<i>manno</i>- and <scp>D</scp>-<i>glycero</i>-<scp>D</scp>-<i>manno</i>-heptoses: Analysis of the structure and conformation by high-resolution NMR spectroscopy (2002)

Previous studies from our laboratory have indicated that the lipopolysaccharide (LPS) from Mannheimia haemolytica serotype A1 contains both L-glycero-D-manno-heptose and D-glycero-D-manno-heptose residues. NMR methods making use of 1D 1 H selective excitation and 2D ( 1 H, 13 C) and ( 1 H, 31 P) heteronuclear experiments were used for the structural determination of the major core oligosaccharide components of the deacylated low-molecular-mass LPS obtained following sequential treatment with anhydrous hydrazine and aq KOH. The core oligosaccharide region was found to be composed of a branched octasaccharide linked to the deacylated lipid A moiety via a 3-deoxy-4-phospho-D-manno-oct-2-ulosonate residue having the structure,[Formula: see text]Heterogeneity was found to be present at several linkages. NMR methods were devised to distinguish between the diastereomeric forms of the heptose residues. Synthesized monosaccharides of L-D- and D-D-heptose were used as model compounds for analysis of the 1 H and 13 C NMR chemical shifts and proton coupling constants. Molecular modeling using a Monte Carlo method for conformational analysis of saccharides was used to determine the conformation of the inner core of the oligosaccharide and to establish the stereochemical relationships between the heptoses.Key words: LPS, NMR, conformation, oligosaccharide, heptose.
On Some Fundamental Issues in Political Economy: An Exchange of Correspondence (1975)

Abstract The letter printed below are published by the correspondents with the hope that they will stimulate discussion and creative rethinking about some fundamental and interesting issues that are often ignored in the conduct of economic analysis and research and the application of traditional tools and concepts. The issues, we are aware, are not novel. Our conflicts and apprehensions replicate, in varying degree, past methodological controversies. But we are convinced that the fundamental nature of the topics argued warrants continuing re-examination. Just as each generation of economists has the burden of interpreting for itself the history of the discipline, each generation also confronts, directly or indirectly, the problem of the methodological foundations of economic analysis. Moreover, as the discussions in the letters illustrate, methodological issues are closely related to normative or policy positions, although the relations are often ambiguous and equivocal.
Voxel Selection Framework in Multi-Voxel Pattern Analysis of fMRI Data for Prediction of Neural Response to Visual Stimuli (2014)

Multi-voxel pattern analysis (MVPA) of functional magnetic resonance imaging (fMRI) data is an emerging approach for probing the neural correlates of cognition. MVPA allows cognitive states to be modeled as distributed patterns of neural activity and classified according to stimulus conditions. In practice, building a robust, generalizable classification model can be challenging because the number of voxels (features) far exceeds the number of stimulus instances/data observations. To avoid model overfitting, there is a need to select informative voxels before building a classification model. In this paper, we propose a robust feature (voxel) selection framework using mutual information (MI) and partial least square regression (PLS) to establish an informativeness index for prioritizing selection of voxels based on the degree of their association to the experimental conditions. We evaluated the robustness of our proposed framework by assessing performance of standard classification algorithms, when combined with our feature selection approach, in a publicly-available fMRI dataset of object-level representation widely used to benchmark MVPA performance (Haxby, 2001). The computational results suggest that our feature selection framework based on MI and PLS drastically improves the classification accuracy relative to those previously reported in the literature. Our results also suggest that highly informative voxels may provide meaningful insight into the functional-anatomic relationship of brain activity and stimulus conditions.
Novel Clinical and Diagnostic Aspects of Antineutrophil Cytoplasmic Antibodies (2014)

Antineutrophil cytoplasmic antibodies (ANCA) are the serological hallmark of some idiopathic systemic vasculitides. Besides the investigation of ANCA-associated vasculitis (AAV) and constant effort for a standardized nomenclature and classification of the AAV, a main focus of research during the last few years has been to constantly improve the performance of enzyme immunoassays. With the latest so called third generation ELISA, this goal seemed to be fulfilled. The International Consensus Statement on Testing and Reporting of ANCA gave recommendations for standardized strategies for the serological diagnosis of ANCA. New developments now target the system immanent drawbacks of the respective diagnostic methods, be it the need for batching and the long time to result for ELISA, or the high likelihood of error and subjectivity of indirect immunofluorescence (IIF). Random access technology and multiplexing for solid phase assays as well as digital imaging for IIF are tools which may help to expedite and simplify routine diagnostics in the lab and in emergency settings. Recent findings indicate that PR3-ANCA have clinical utility beyond the diagnosis of AAV. PR3-ANCA can also serve as an aid for the differentiation between ulcerative colitis (UC) and Crohn's disease (CrD) and the stratification of UC patients. This review provides a detailed review of what is known about ANCA and highlights the latest research and state-of-the-art developments in this area.
Full-length CD4 electroinserted in the erythrocyte membrane as a long-lived inhibitor of infection by human immunodeficiency virus. (1991)

Recombinant full-length CD4 expressed in Spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (RBC) membranes. Of the inserted CD4, 70% was "correctly" oriented as shown by fluorescence quenching experiments with fluorescein-labeled CD4. The inserted CD4 displayed the same epitopes as the naturally occurring CD4 in human T4 cells. Double-labeling experiments (125I-CD4 and 51Cr-RBC) showed that the half-life of CD4 electroinserted in RBC membrane in rabbits was approximately 7 days. Using the fluorescence dequenching technique with octadecylrhodamine B-labeled human immunodeficiency virus (HIV)-1, we showed fusion of the HIV envelope with the plasma membrane of RBC-CD4, whereas no such fusion could be detected with RBC. The dequenching efficiency of RBC-CD4 is the same as that of CEM cells. Exposure to anti-CD4 monoclonal antibody OKT4A, which binds to the CD4 region that attaches to envelope glycoprotein gp120, caused a significant decrease in the dequenching of fluorescence. In vitro infectivity studies showed that preincubation of HIV-1 with RBC-CD4 reduced by 80-90% the appearance of HIV antigens in target cells, the amount of viral reverse transcriptase, and the amount of p24 core antigen produced by the target cells. RBC-CD4, but not RBCs, aggregated with chronically HIV-1-infected T cells and caused formation of giant cells. These data show that the RBC-CD4 reagent is relatively long lived in circulation and efficient in attaching to HIV-1 and HIV-infected cells, and thus it may have value as a therapeutic agent against AIDS.
Development of finasteride polymer microspheres for systemic application in androgenic alopecia (2019)

The use of finasteride for alleviating hair loss has been investigated, and it has been applied as an oral dose medication. However, due to the inconvenience of daily drug administration over long period of time, novel controllable finasteride delivery has been actively investigated. As a novel method of finasteride delivery, the development of finasteride‑loaded microspheres for subcutaneous administration is becoming increasingly pharmaceutically important. Therefore, the present study aimed to use finasteride‑loaded microspheres in a controlled manner in an attempt to overcome the limitations of the oral administration of finasteride and to cause fewer adverse effects. Finasteride‑loaded microspheres containing poly(lactic‑co‑glycolic acid) and finasteride at a ratio of 4:1 were prepared, and a testosterone‑induced androgenic alopecia mouse model was used. Following observation for 10 weeks, the percentage hair growth was 86.7% (total hair growth 60%, partial hair growth 26.7%) in the orally‑applied finasteride‑treated group as a positive control, and 93.3% (total hair growth 60%, partial hair growth 33.3%) in the finasteride‑loaded microspheres‑treated group. Serum dihydrotestosterone levels began to decrease at week 6 in the orally‑applied finasteride‑ and finasteride‑loaded microsphere‑treated groups. In addition, the finasteride‑loaded microspheres‑treated group exhibited similar follicular number, follicular length, anagen/telogen ratio and hair bulb diameter values to those of the orally‑applied finasteride‑treated group. Furthermore, the finasteride‑loaded microspheres increased the activities of phosphoinositide 3‑kinase/protein kinase B and Wnt/β‑catenin in relation to hair follicle cell growth signaling in mouse skin, and suppressed the apoptosis of hair follicle cells by reducing the expression of transforming growth factor‑β2 and caspase‑3, which are indicators of apoptosis. In conclusion, the administration of a single injection of finasteride‑loaded microspheres was effective in treating testosterone‑induced alopecia. Furthermore, it led to equivalent hair growth effects when compared with orally‑applied finasteride, thus revealing the possibility of effective treatment via different routes of administration.
High Performance Computing Productivity Model Synthesis (2004)

The Defense Advanced Research Projects Agency (DARPA) High Productivity Computing System (HPCS) program is developing systems that deliver increased value to users at a rate commensurate with the rate of improvement in the underlying technologies. For example, if the relevant technology was silicon, the goal of such a system would be to double in productivity (or value) every 18 months, following Moore's law. The key questions are how we define and measure productivity, and what the underlying technologies that affect productivity are. The goal of this paper is to synthesize from several different productivity models a single model that captures the main features of all the models. In addition we will start the process of putting the model on an empirical foundation by incorporating selected results from the software engineering and high performance computing (HPC) communities. An asymptotic analysis of the model is conducted to check that it makes sense in certain special cases. The model is extrapolated to a HPC context and several examples are explored, including HPC centers, HPC users, and interactive grid computing. Finally, the model hints at a profoundly different way of viewing HPC systems, where the user must be included in the equation, and innovative hardware is a key aspect to lowering the very high costs of HPC software.
Tissue Engineering in the Trachea (2013)

This review summarizes efforts to generate an autologous tissue-engineered trachea (TET) using various biomaterial or cell sources to make tracheal cartilage to form the structural components of a functional tracheal replacement. Biomechanical assessments of the TET showed that the cartilage stiffness was excellent in the nude models; however, the sheep autologous TET did not provide sufficient support and collapsed easily. As a result, tissue engineering technology is still far from allowing the functional recovery of patients who suffer from severe tracheal disease. On the other hand, there are several clinical reports seeding cells to decellularized tissue using tissue engineering techniques. However, the working mechanisms of the tissue-engineered trachea remain unclear. Nevertheless, we believe that the field of tissue engineering has great potential for surmounting these obstacles and allowing us to generate functional tracheal replacements in the near future.
<i>Chlamydia trachomatis</i> Mip‐like protein has peptidylprolyl <i>cis/trans</i> isomerase activity that is inhibited by FK506 and rapamycin and is implicated in initiation of chlamydial infection (1993)

The Mip-like protein of Chlamydia trachomatis has sequence similarity with both the Mip protein of Legionella pneumophila, a virulence factor necessary for optimal intracellular infection, and FK506-binding proteins (FKBPs) of both prokaryotic and eukaryotic origin. FKBPs contain a site for peptidyl-prolyl cis/trans isomerase activity, which is blocked upon binding of the drugs, FK506 or rapamycin. In this paper we report that the recombinant chlamydial Mip-like protein exhibits a peptidyl-prolyl cis/trans isomerase activity which is inhibited by either rapamycin or FK506. To assess the role of the Mip-like protein in chlamydial infection, rapamycin or FK506 (25 microM), were used in either treatment of chlamydial organisms prior to inoculation, or were present at different intervals through the infection. Pretreatment of organisms alone reduced infectivity for McCoy cells by 30%, with inhibition rising to 80% on more prolonged exposure from 0 to 8h and 8 to 16h post-inoculation and declining thereafter. When drug was present during the developmental cycle at intervals from 0 to 24h post-inoculation abnormal chlamydiae were induced in residual inclusions. The results suggest that inhibition of the isomerase of the Mip-like protein interferes with one or more early events in the infective process that determine productive intracellular infection.
A novel PFC scheme for AC to DC converter with reduced losses (2002)

A novel single phase AC to DC power factor correction (PFC) scheme named boost bypass control (BBC) is presented. Here, the conventional boost PFC circuit during a sinewave input current is bypassed during input AC voltage peaks by a rectifier resulting in lower rated power components and acceptable power factor. The proposed control scheme controls directly the power shared between the boost path and the bypass path. The viability of such a scheme is established theoretically and experimentally. Practical implementation of the BBC scheme as well as experimental results are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Dialysis‐Induced Oxidative Stress: Biological Aspects, Clinical Consequences, and Therapy (2001)

Oxidative stress, which results from a rupture in the natural balance between pro- and antioxidant systems, is considered as a major factor in dialysis-associated morbidity and mortality. Emerging pharmacologic and dialytic antioxidant therapeutic and dialysis strategies should enable us to reduce the harmful consequences of oxidative stress in dialysis patients. Moreover, since there is increasing evidence of oxidative stress long before the initiation of maintenance dialysis, antioxidant therapeutic strategies should probably be developed very early in the course of renal failure.
Mixed Chimerism and Growth Factors Augment β Cell Regeneration and Reverse Late-Stage Type 1 Diabetes (2012)

Type 1 diabetes (T1D) results from an autoimmune destruction of insulin-producing β cells. Currently, islet transplantation is the only curative therapy for late-stage T1D, but the beneficial effect is limited in its duration, even under chronic immunosuppression, because of the chronic graft rejection mediated by both auto- and alloimmunity. Clinical islet transplantation is also restricted by a severe shortage of donor islets. Induction of mixed chimerism reverses autoimmunity, eliminates insulitis, and reverses new-onset but not late-stage disease in the nonobese diabetic (NOD) mouse model of T1D. Administration of gastrin and epidermal growth factor (EGF) also reverses new-onset but not late-stage T1D in this animal model. Here, we showed that combination therapy of induced mixed chimerism under a radiation-free nontoxic anti-CD3/CD8 conditioning regimen and administration of gastrin/EGF augments both β cell neogenesis and replication, resulting in reversal of late-stage T1D in NOD mice. If successfully translated into humans, this combination therapy could replace islet transplantation as a long-term curative therapy for T1D.
Prostaglandin-E2 enhances EPO-mediated STAT5 transcriptional activity by serine phosphorylation of CREB (2002)

Erythroid colony formation in response to erythropoietin (EPO) stimulation is enhanced by costimulating the cells with prostaglandin-E2 (PGE2). The present study further analyzed the underlying mechanisms and demonstrated that EPO-mediated STAT5 transactivation in the erythroid AS-E2 cell line was enhanced 6-fold by PGE2 (10 microM), without affecting the STAT5 tyrosine phosphorylation or STAT5-DNA binding. Moreover, the PGE2-enhancing effect was independent of STAT5 serine phosphorylation. In AS-E2 cells STAT5 is constitutively phosphorylated on Ser780 (STAT5A) and EPO-dependently phosphorylated on Ser726/731 (STAT5A/STAT5B), but overexpression of STAT5 serine mutants did not affect STAT5 transactivation. In addition, PGE2 did not affect STAT5 serine phosphorylation. Instead, the stimulatory effect of PGE2 on STAT5 signaling could be mimicked by dibutyryl-cyclic adenosine monophosphate (cAMP) and the phosphodiesterase inhibitor IBMX, suggesting that the effect was mediated by cAMP. Activation of the cAMP pathway resulted in cAMP-response element binding protein (CREB) phosphorylation, which was sustained in the presence of EPO plus PGE2 and transient on EPO stimulation alone. The costimulatory effect of PGE2 on EPO-mediated STAT5 transactivation was inhibited by overexpression of serine-dead CREB or protein kinase A (PKA) inhibitor (PKI), in contrast to EPO-mediated transactivation, which was PKA independent. Furthermore, CREB-binding protein (CBP)/p300 was shown to be involved in EPO-mediated STAT5 transactivation, and a CBP mutant with increased affinity for CREB resulted in an additional enhancement of the PGE2 effect. Finally, we demonstrated that the STAT5 target genes Bcl-X, SOCS2, and SOCS3 were up-regulated by costimulation with PGE2. In summary, these studies demonstrate that PGE2 enhancement of EPO-induced STAT5 transactivation is mediated by the cAMP/PKA/CREB pathway.