gebhart/patrepeval huggingface dataset:
Modification of <i>Helicobacter pylori</i> outer membrane protein expression during experimental infection of rhesus macaques (2004)

Clinical isolates of Helicobacter pylori show marked diversity, which may derive from genomic changes that occur during the often lifelong association of the bacterium with its human host. We used the rhesus macaque model, together with DNA microarrays, to examine genomic changes in H. pylori that occur early during experimental infection. Microarray analysis showed that H. pylori recovered from challenged macaques had deleted babA, a member of a large family of paralogous outer membrane proteins (OMPs) that mediates attachment of H. pylori to the Lewis B blood group antigen on gastric epithelium. In some cases the babA gene was replaced by babB, an uncharacterized OMP that is closely related to babA. In other cases the babA gene was present but was not expressed because of alteration in dinucleotide CT repeats in the 5' coding region. In either case, strains lacking babA did not adhere to Lewis B, which is expressed on macaque gastric epithelium. Absence of babA and duplication of babB was also seen in H. pylori isolates derived from human clinical samples, suggesting that this gene conversion event is not unique to experimentally infected rhesus monkeys. These results demonstrate in real time with a relevant animal model that H. pylori regulates OMP expression in vivo by using both antigenic variation and phase variation. We suggest that changes in babA and babB after experimental infection of macaques represent a dynamic response in the H. pylori outer membrane that facilitates adherence to the gastric epithelium and promotes chronic infection.
An Observational Study from Long-Term AAV Re-administration in Two Hemophilia Dogs (2018)

Adeno-associated virus (AAV) vectors have been successfully applied in hemophilia clinical trials. However, this approach is limited to patients without AAV-neutralizing antibodies (NAbs). In this study, we explored the feasibility of AAV re-administration in hemophilia A dogs treated initially 8 years ago with AAV8.canine FVIII. After the re-administration in two NAb-negative dogs with AAV8 vectors carrying human factor VIII (hFVIII), along with the proteasome inhibitor bortezomib, we observed a phenotypic improvement in both dogs that persisted in one dog. Phenotypic improvement disappeared at 59 days after re-administration in the other dog, and specific cytotoxic T lymphocytes (CTLs) to the capsid were detected at day 17, but not to hFVIII. hFVIII inhibitors were observed at day 59 and gradually increased. Mechanistic studies demonstrated an increase in pro-inflammatory cytokines, a decrease in immunomodulatory cytokines, as well as lower Tregs after re-administration. These results suggest that hFVIII inhibitor development may contribute to the therapeutic failure via immune response activation. Interestingly, it takes about 30-50 days for AAV NAb titers to decrease by half. Collectively, this study suggests that re-administration of the same AAV serotype after long-term follow-up is feasible and that the study of AAV NAb kinetics will provide important information for predicating the efficacy of re-administration.
Comparative sorption kinetic studies of dye and aromatic compounds onto fly ash (1999)

Abstract A pseudo‐second order and a pseudo‐first order mechanism for the sorption of Omega Chrome Red ME as well as o‐cresol and p‐nitrophenol onto fly ash have been compared. Intraparticle diffusion processes and chemical sorption processes for the sorption also have been studied. The batch data for the sorption of Omega Chrome Red ME, o‐cresol and p‐nitrophenol onto fly ash have been analysed to predict the rate constant of sorption based on the assumption of a pseudo‐second order mechanism. The equilibrium capacity and initial sorption rate have also been determined to study the effect of initial solute concentration and temperature on the sorption process. An activation energy of sorption has also been evaluated using the pseudo‐second order rate constants. Key words: Fly ashkineticssorptiondyeo‐cresol p‐ nitrophenol
Equine Infectious Anemia Virus Utilizes Host Vesicular Protein Sorting Machinery during Particle Release (2003)

A final step in retrovirus assembly, particle release from the cell, is modulated by a small motif in the Gag protein known as a late domain. Recently, human immunodeficiency virus type 1 (HIV-1) and Moloney murine leukemia virus (M-MuLV) were shown to require components of the cellular vacuolar protein sorting (VPS) machinery for efficient viral release. HIV-1 interacts with the VPS pathway via an association of HIV-1 Gag with TSG101, a component of the cellular complexes involved in VPS. Equine infectious anemia virus (EIAV) is unique among enveloped viruses studied to date because it utilizes a novel motif, YPDL in Gag, as a late domain. Our analysis of EIAV assembly demonstrates that EIAV Gag release is blocked by inhibition of the VPS pathway. However, in contrast to HIV-1, EIAV Gag release is insensitive to TSG101 depletion and EIAV particles do not contain significant levels of TSG101. Finally, we demonstrate that fusing EIAV Gag directly with another cellular component of the VPS machinery, VPS28, can restore efficient release of an EIAV Gag late-domain mutant. These results provide evidence that retroviruses can interact with the cellular VPS machinery in several different ways to accomplish particle release.
Metabolism of arginine in lactating rat mammary gland (1977)

Significant activities of the four enzymes needed to convert arginine into proline and glutamate (arginase, ornithine aminotransferase, pyrroline-5-carboxylate reductase and pyrroline-5-carboxylate dehydrogenase) develop co-ordinately in lactating rat mammary glands in proportion to the increased production of milk. No enzymes were detected to carry out the reactions of proline oxidation or reduction of glutamate to pyrroline-5-carboxylate. Minces of the gland converted ornithine into proline and into glutamate plus glutamine. These conversions increased during the cycle of lactation in proportion to the increased milk production and to the content of the necessary enzymes. The minced gland did not convert labelled ornithine into citrulline, confirming the absence from the gland of a functioning urea cycle, and did not convert labelled proline or glutamate into ornithine. A metabolic flow of labelled arginine to proline and glutamate in mammary gland was confirmed in intact animals with experiments during which the specific radioactivity of proline in plasma remained below that of the proline being formed from labelled arginine within the gland. It was concluded that arginase in this tissue had a metabolic role in the biosynthesis of extra proline and glutamate needed for synthesis of milk proteins.
Salermide up‐regulates death receptor 5 expression through the ATF4‐ATF3‐CHOP axis and leads to apoptosis in human cancer cells (2011)

Sirtuins (a class III histone deacetylase) have emerged as novel targets for cancer therapy. Salermide, a reverse amide compound that inhibits Sirtuin 1 (Sirt1) and Sirtuin 2 (Sirt2), has been shown to induce apoptosis in human cancer cells. The mechanism underlying cellular apoptotic signalling by salermide remains unclear. In this study, we show that salermide up-regulates the expression of death receptor 5 (DR5) in human non-small cell lung cancer (NSCLC) cells. Blocking DR5 expression by gene silencing technology results in a decrease in activated forms of several pro-apoptotic proteins (caspase-8, caspase-9, caspase-3, PARP). Increasing DR5 protein expression correlates with salermide-induced apoptosis in human NSCLC cells. We discovered that IRE-1α, Bip, activating transcription factor 3 (ATF4), activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP) are induced by salermide, which suggests that DR5-dependent apoptosis is induced by endoplasmic reticulum stress. Moreover, knockdown of Sirt1 and Sirt2 expression resulted in up-regulation of ATF4, CHOP and DR5. Transfected NSCLC cells with ATF4, ATF3 or CHOP siRNA results in a decline in pro-apoptotic proteins (such as caspase-8, caspase-9, caspase-3 and PARP) despite salermide treatment. We demonstrate that salermide induces expression of ATF4, and ATF4 up-regulates ATF3 and subsequently modulates CHOP. This suggests that DR5 is modulated by the ATF4-ATF3-CHOP axis in NSCLC after Sirt1/2 inhibition or salermide treatment. This study highlights the importance of DR5 up-regulation in apoptosis induced by Sirt1/2 inhibition and elucidates the underlying mechanism in human NSCLC cells.
Caspase-14 Is a Novel Developmentally Regulated Protease (1998)

Caspases are a family of cysteine proteases related to interleukin-1 converting enzyme (ICE) and represent the effector arm of the cell death pathway. The zymogen form of all caspases is composed of a prodomain plus large and small catalytic subunits. Herein we report the characterization of a novel caspase, MICE (for mini-ICE), also designated caspase-14, that possesses an unusually short prodomain and is highly expressed in embryonic tissues but absent from all adult tissues examined. In contrast to the other short prodomain caspases (caspase-3, caspase-6, and caspase-7), MICE preferentially associates with large prodomain caspases, including caspase-1, caspase-2, caspase-4, caspase-8, and caspase-10. Also unlike the other short prodomain caspases, MICE was not processed by multiple death stimuli including activation of members of the tumor necrosis factor receptor family and expression of proapoptotic members of the bcl-2 family. Surprisingly, however, overexpression of MICE itself induced apoptosis in MCF7 human breast cancer cells, which was attenuated by traditional caspase inhibitors.
Texture features for land cover change detection at 250 m resolution-an application of machine learning feature subset selection (2002)

Texture measures have been widely studied in addition to spectral features to characterize land cover type. Texture measures can be used to update land cover maps and enhance classification accuracy. Most previous studies have focussed only on fine resolution data such as Landsat, SPOT and Synthetic Aperture Radar imagery. Texture analysis at coarser resolution (250 m) has not been investigated because data are only beginning to become available with the launch of MODIS (Moderate Resolution Imaging Spectroradiometer). To meet the need of global change research, the MODIS instrument is planned to provide global coverage at 250 m resolution in the red and infrared bands. Early warning of human-induced land cover changes, such as urbanization and deforestation, is expected from MODIS. This paper examines whether texture analysis at this scale would be useful for detecting changes. There are at least two issues in using texture analysis: 1) which texture measures should be used, and 2) what window size is most appropriate for capturing textures. In this study we test a large number of texture measures and window sizes on MODIS data simulated from Landsat data using the data mining technique of feature selection.
Improved Stability of OPALMON Tablets under Humid Conditions IV: Effect of Polysaccharides and Disintegrants on the Stability and Dissolution Property of OPALMON Tablets (2008)

We studied the effects of dextran, dextrin, and disintegrants on the chemical stability of Opalmon tablets containing Limaprost-alfadex (Limaprost/alpha-cyclodextrin complex) and found that the addition of dextran or dextrin significantly improved the chemical stability of Opalmon tablets under high humidity, compared to lactose. We also examined how dextran stabilizes Limaprost in Opalmon tablets and studied the formulation of Opalmon tablets in order to achieve higher chemical stability, rapid dissolution and reduced stickiness. The results suggested that dextran increases stabilization after moisture adsorption by decreasing the dissociation of Limaprost-alfadex to the free drug and alpha-cyclodextrin in the dextran matrix, when compared with the lactose matrix. The stickiness of Opalmon tablets containing dextran and dextrin was negligible when dextran and dextrin amounted to less than 20% of the formulation. By selecting a proper disintegrant, we obtained Opalmon tablets with higher chemical stability and rapid dissolution properties.
Continuous Infusion or Bolus Injection in Cancer Chemotherapy (1983)

Continuous intravenous infusion of anticancer drugs is being incorporated into more experimental chemotherapy protocols. The rationale for use of continuous infusions generally includes the restriction of cytotoxic mechanisms of a drug to a specific phase of the cell cycle and the short half-life of some drugs. Two such agents are cytarabine and bleomycin; continuous exposure to these drugs greatly increases their antitumor effects in cell cultures and animal models. Toxicity to normal tissues may also be reduced by continuous infusion, notably the pulmonary toxicity of bleomycin, the cardiac toxicity of doxorubicin, and the myelosuppression of fluorouracil. Recent advances in infusion pump technology have made continuous intravenous infusion therapy more practical. Unfortunately, most clinical studies of the continuous infusion of anticancer drugs have been uncontrolled. Further randomized, controlled clinical trials comparing schedules of drug administration are necessary before definitive recommendations can be made.
Bluetooth enabled printer adapter using raspberry pi (2015)

The common data printing procedure has to use notebook or desktop computer as a relay medium, first receiving the data from mobile and then sending it to printer using an appropriate printing Method. This is rather cumbersome. This paper proposes a design of a device which directly prints the data stored inside the Mobile without the assistance of a notebook or desktop computer. The design compromises Raspberry pi having Debian flavor of Unix. The configured Bluetooth dongle accept file from Mobile and gives to RPi. The Printer driver is a program to control a printer that is connected to your system. The CUPs installed on RPi do the print job.
A Primal-Dual Randomized Algorithm for Weighted Paging (2007)

In the weighted paging problem there is a weight (cost) for fetching each page into the cache. We design a randomized O(log k) -competitive online algorithm for the weighted paging problem, where k is the cache size. This is the first randomized o(k)-competitive algorithm and its competitiveness matches the known lower bound on the problem. More generally, we design an O(log(k/(k - h + I)))-competitive online algorithm for the version of the. problem where, the online algorithm has-cache size k and the online algorithm has cache size h les k. Weighted paging is a special case (weighted star metric) of the well known k-server problem for which it is a major open question whether randomization can be useful in obtaining sub-linear competitive algorithms. Therefore, abstracting and extending the insights from paging is a key step in the resolution of the k-server problem. Our solution for the weighted paging problem is based on a two-step approach. In the first step we obtain an O(log k)-competitive fractional algorithm which is based on a novel online primal-dual approach. In the second step we. obtain a randomized algorithm by rounding online the fractional solution to an actual distribution on integral cache, solutions. We conclude with a randomized O(log N)-competitive algorithm for the well studied Metrical Task System problem (MTS) on a metric defined by a weighted star on N leaves, improving upon a previous O(log <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> N)-competitive algorithm of Blum et al. [9].
Improving the performance of Luffa Hash Algorithm (2010)

Luffa is a new hash algorithm that has been accepted for round two of the NIST hash function competition SHA-3. Computational efficiency is the second most important evaluation criteria used to compare candidate algorithms. In this paper, we describe a fast software implementation of the Luffa hash algorithm for the Intel Core 2 Duo platform. We explore the use of the perfect shuffle operation to improve the performance of 64-bit implementation and 128-bit implementation with the Intel Supplemental SSSE3 instructions. In addition, we introduce a new way of implementing Luffa based on a Parallel Table Lookup instruction. The timings of our 64-bit implementation (C code) resulted in a 16 to 32% speed improvement over the previous fastest implementation.
Loss of the SMRT/NCoR2 Corepressor Correlates with JAG2 Overexpression in Multiple Myeloma (2009)

Multiple myeloma (MM) is a clonal B-cell neoplasm that accounts for 10% of all malignant hematologic neoplasms and that affects terminally differentiated B cells (i.e., plasma cells). It is now well recognized that the cytokine interleukin-6 (IL-6) is a major cytokine that promotes the proliferation of malignant plasma cells in MM. The IL-6 gene can be regulated by the NOTCH genes products. We have previously shown that the NOTCH ligand, JAG2, is overexpressed in MM. To investigate the mechanism(s) leading to JAG2 overexpression in MM, we assessed potential epigenetic modifications of the JAG2 promoter. We showed that the JAG2 promoter region is aberrantly acetylated in MM cell lines and patient samples. The acetylation state of histones is regulated by the recruitment of histone deacetylases (HDAC). HDACs are typically recruited to promoter regions through interaction with nuclear corepressors such as SMRT. SMRT levels were therefore investigated. Interestingly, MM cell lines and patient samples presented significantly reduced SMRT levels. The experiments suggest a correlation between constitutive acetylation of the JAG2 core promoter in the MM cell lines and reduced levels of the SMRT corepressor that recruits HDAC to promoter regions. Finally, SMRT function restoration induced JAG2 down-regulation as well as MM cell apoptosis.
The NKp46 Receptor Contributes to NK Cell Lysis of Mononuclear Phagocytes Infected with an Intracellular Bacterium (2002)

We used human tuberculosis as a model to investigate the role of NK cytotoxic mechanisms in the immune response to intracellular infection. Freshly isolated NK cells and NK cell lines from healthy donors lysed Mycobacterium tuberculosis-infected monocytes to a greater extent than uninfected monocytes. Lysis of infected monocytes was associated with increased expression of mRNA for the NKp46 receptor, but not the NKp44 receptor. Antisera to NKp46 markedly inhibited lysis of infected monocytes. NK cell-mediated lysis was not due to reduced expression of MHC class I molecules on the surface of infected monocytes or to enhanced production of IL-18 or IFN-gamma. NK cell lytic activity against M. tuberculosis-infected monocytes and NKp46 mRNA expression were reduced in tuberculosis patients with ineffective immunity to M. tuberculosis compared with findings in healthy donors. These observations suggest that 1) the NKp46 receptor participates in NK cell-mediated lysis of cells infected with an intracellular pathogen, and 2) the reduced functional capacity of NK cells is associated with severe manifestations of infectious disease.
A Review on Single Sign on Enabling Technologies and Protocols (2016)

In today's digital era, users are increasingly accessing countless number of applications every day. For accessing these services, the users first have to authenticate themselves and need to maintain a separate set of username and password for each application. This led to the development of Single Sign On (SSO). This paper presents review on SSO enabling technologies and discusses SSO architectures, protocols and analysis related to growing use of SSO.
Fog‐enabled vehicular networks: A new challenge for mobility management (2019)

With the increasing presence of latency‐critical applications and services along with the high demand for ubiquitous connectivity, a new challenge arise in the design of the next generation of vehicular networks. The requirements of low latency services and powerful computational functionalities have decreased the performance of cloud centers, being unable to satisfy the fast growing number of vehicles on roads and road assistance based applications. Vehicular Fog Networks is an emerging network architecture that elevates the burden on the cloud and shifts Cloud services to the edge of the network. However, the integration of fog‐computing and vehicular networks raises several issues and challenges, including mobility management. In this paper, we provide insights into the latest solutions for mobility management protocols in fog‐enabled vehicular networks. Then, we explore related open issues and point out future research directions.
High-strength carbon nanotube buckypaper composites as applied to free-standing electrodes for supercapacitors (2013)

Buckypaper is an attractive candidate material for free-standing electrodes in supercapacitors due to its high electrochemical performance, light weight, and thin dimensions. At present, however, free-standing buckypapers exhibit severe limitations in terms of product quality, especially mechanical properties, which hinder their commercial applications. We here report a new method of co-packaging buckypaper with conducting polymer and thermosetting resin to fabricate cellular SWNT buckypaper materials with excellent mechanical properties, high electrical conductivity, and enhanced electrochemical properties. This new fabrication method involves wrapping of the as-prepared buckypaper with a uniform coaxial coating of polypyrrole (PPy) on the individual SWNT or SWNT bundle surfaces via a pulsed electrochemical deposition method, followed by further packaging with cyanate ester resin via a full dip infiltration. The resulting material exhibits a significant improvement in mechanical properties (improvement over unmodified buckypaper of about 400% in tensile modulus and strength) and enhanced electrochemical performance (320 F g−1 at a current density of 1 A g−1) without sacrificing electrical and thermal properties. This material is a promising candidate for use as a free-standing electrode material in small-size, light-weight, and high-temperature supercapacitors.
Clinical observations of a titanium-alloy prefabricated post. (1990)

Clinical observations led to the development of this article in order that the dental profession may become cognizant of the ethereal radiographic appearance of titanium-alloy posts.
Macromolecular Properties of Polyvinylpyrrolidone:Molecular Weight Distribution (1953)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMacromolecular Properties of Polyvinylpyrrolidone:Molecular Weight DistributionL. E. Miller and F. A. HammCite this: J. Phys. Chem. 1953, 57, 1, 110–122Publication Date (Print):January 1, 1953Publication History Published online1 May 2002Published inissue 1 January 1953https://pubs.acs.org/doi/10.1021/j150502a024https://doi.org/10.1021/j150502a024research-articleACS PublicationsRequest reuse permissionsArticle Views364Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts