gebhart/patrepeval huggingface dataset:
Temperature-sensitive mutations in the vaccinia virus H4 gene encoding a component of the virion RNA polymerase (1992)

Four previously isolated temperature-sensitive (ts) mutants of vaccinia virus WR (ts1, ts31, ts55, and ts58) comprising a single complementation group (R. C. Condit, A. Motyczka, and G. Spizz, Virology 128:429-443, 1983) have been mapped by marker rescue to the H4L open reading frame located within the genomic HindIII-H DNA fragment. The H4 gene is predicted to encode a 93.6-kDa polypeptide expressed at late times during infection. Nucleotide sequence alterations responsible for thermolabile growth lead to amino acid substitutions in the H4 gene product. All four ts alleles display "normal" patterns of early and late viral protein synthesis at the nonpermissive temperature (40 degrees C). Mature virion particles, microscopically indistinguishable from wild-type virions, are produced in the cytoplasm of cells infected with ts1 at 40 degrees C. Western immunoblot analysis localizes the H4 protein to the virion core. After solubilization from cores, the H4 protein is associated during purification with transcriptionally active vaccinia virus DNA-dependent RNA polymerase.
Alanine scan of the opioid peptide dynorphin B amide (2017)

To date structure-activity relationship (SAR) studies of the dynorphins (Dyn), endogenous peptides for kappa opioid receptors (KOR), have focused almost exclusively on Dyn A with minimal studies on Dyn B. While both Dyn A and Dyn B have identical N-terminal sequences, their C-terminal sequences differ, which could result in differences in pharmacological activity. We performed an alanine scan of the non-glycine residues up through residue 11 of Dyn B amide to explore the roles of these side chains in the activity of Dyn B. The analogs were synthesized by fluorenylmethyloxycarbonyl (Fmoc)-based solid phase peptide synthesis and evaluated for their opioid receptor affinities and opioid potency and efficacy at KOR. Similar to Dyn A the N-terminal Tyr<sup>1</sup> and Phe<sup>4</sup> residues of Dyn B amide are critical for opioid receptor affinity and KOR agonist potency. The basic residues Arg<sup>6</sup> and Arg<sup>7</sup> contribute to the KOR affinity and agonist potency of Dyn B amide, while Lys<sup>10</sup> contributes to the opioid receptor affinity, but not KOR agonist potency, of this peptide. Comparison to the Ala analogs of Dyn A (1-13) suggests that the basic residues in the C-terminus of both peptides contribute to KOR binding, but differences in their relative positions may contribute to the different pharmacological profiles of Dyn A and Dyn B. The other unique C-terminal residues in Dyn B amide also appear to influence the relative affinity of this peptide for KOR versus mu and delta opioid receptors. This SAR information may be applied in the design of new Dyn B analogs that could be useful pharmacological tools.
The effect of epidural analgesia on the second stage of labor. (1998)

A retrospective review of 202 randomly selected records of parturient labors examined the relationship between cervical dilation at epidural analgesia administration and length of the second stage of labor. The epidural group received bupivacaine 0.11% or 0.125% with sufentanil 1 to 2 micrograms/mL using a Bard Patient Controlled Anesthesia II pump. Labor management and outcomes were compared with a nonepidural group who chose unmedicated childbirth, intravenous narcotics, or pudendal block. A significant inverse correlation was found between cervical dilation at epidural administration and second-stage length in labors that did not use oxytocin. However, linear regression explained only 13.5% of the variance, leaving 86.5% unexplained. In labors in the epidural group that used oxytocin, cervical dilation at epidural administration was not correlated with second-stage length. The epidural group experienced a significantly longer mean length of the second stage. Labors in the epidural group were 3.5 times more likely to have oxytocin induction or augmentation and 4.5 times more likely to experience instrument-assisted delivery. There were no significant differences in Apgar scores between the two infant groups.
Dynamic Performance of a Compression Thermoelastic Cooling Air-Conditioner under Cyclic Operation Mode (2014)

Traditional vapor compression cooling refrigerants are considered as high global-warming-potential (GWP) gases which face more and more legislation pressure nowadays. As an alternative option other than using synthetic low GWP refrigerants and natural refrigerants, solid-state cooling technologies show their advantages of zero GWP, and therefore recently attract more attentions. Apart from those well-studied solid-state cooling technologies, such as thermoelectric cooling, thermoacoustic cooling and magnetic cooling, thermoelastic cooling, a.k.a. elastocaloric cooling, is still under development and shows potential of better thermal performance compared with its competitors. In fact, from material perspective, it was estimated by literatures that the COPs for elastocaloric materials are 20% - 120% higher than other solid-state cooling materials under the same operating conditions. This study introduces the thermoelastic cooling concept at the beginning, and then demonstrates one method to operate the compression thermoelastic cooling cycle for air-conditioning application based on the reverse Martensitic phase transition principle. A dynamic model is developed to measure the temperature within the cycle in cyclic operation mode. The cyclic operation is a Brayton cycle consisting of an adiabatic Martensite-Austenite phase transition process, a constant strain heat transfer process between the solid-state refrigerant and the heat sink/source, and a heat recovery process aiming to improve the overall performance. The model uses experimental curve-fitted data to predict the work required to drive the cycle. The coefficient of performance (COP) and cooling capacity are then evaluated based on the power prediction. Parametric studies are conducted to investigate the influence of several significant parameters on the COP and cooling capacity from the model. It is found that the cycle duration parameters, the solid state refrigerant thickness, and operating flow rates are major contributing factors to the performance indices. Based on the parametric study results, a design guideline is then provided for the future researches.
An integrated and federated location service (1995)

With the advent of mobile computing devices and cheap location sensing systems, location information has become an important resource both for mobile and 'desktop' users. We describe some key concepts a scalable ubiquitous location service should be based on. Firstly, we show how such a service can accommodate multiple location sensing systems. Secondly, we discuss hierarchy-based access control policies as flexible and powerful mechanisms to protect users' privacy. Thirdly, we describe server federation as a means to incrementally build a global location service.
Hydrogen Peroxide Sterilization of Hydrophilic Contact Lenses (1975)

A new, simple, and effective procedure for the sterilization of soft contact lenses utilizes hydrogen peroxide, an inexpensive and readily availiable solution.
A new short‐path distillation system applied to the reduction of cholesterol in butter and lard (1994)

Abstract A molecular distillation plant, built particularly to increase the separation efficiency and to obtain safer working conditions, was tested to remove cholesterol from anhydrous butter and lard. A preliminary experiment was carried out with butter to evaluate the fractionation obtained at temperatures between 190 and 250°C and residual pressures between 10 −3 and 10 −4 torr. A second experiment was carried out at 185°C and at the maximum operational vacuum, evaluating the fractionation achieved within a time scale between 30 and 180 min. Cholesterol was almost completely removed during the second hour with minimal loss of low‐molecular weight triglycerides. An experiment was carried out with lard at 250°C and maximum achievable operational vacuum (10 −4 Torr), lasting approximately 6 h, and cholesterol was removed almost completely during the second hour without significant modifications in the triglyceride composition. This situation remained constant throughout the duration of the test.
Isolation, assay, and secretion of individual human neurophysins. (1975)

Human neurophysin was isolated from acetone-dried human posterior pituitaries and separated into two major neurophysin peptides by ion exchange chromatography and into four major peptides by preparative disk gel electrophoresis. Antisera raised in rabbits distinguished only two specific antigenic sites on the isolated neurophysin peptides. Individual sensitive and specific radioimmunoassays for two human neurophysins were developed. These assays were used to measure each neurophysin in unextracted human plasma. The two neurophysins are secreted independently in man. One assay measures a neurphysin that is specifically secreted in response to estrogen administration, estrogen-stimulated neurophysin (ESN). The other assay measures a neurophysin that is specifically secreted in response to cigarette smoking, nicotine-stimulated neurophysin (NSN). The mean ESN is 1.1 ng/ml plus or minus 0.7 SD in women and 1.0 ng/ml plus or minus 0.7 SD in men. The mean NSN is 0.9 ng/ml plus or minus 0.2 SD in women and 0.6 ng/ml plus or minus 0.3 SD in men. It is proposed that these may prove to be a specific human "oxytocinneurophysin," ESN, and a human "vasopressin-neurophysin," NSN.
Counter-example complete verification for higher-order functions (2015)

We present a verification procedure for pure higher-order functional Scala programs with parametric types. We show that our procedure is sound for proofs, as well as sound and complete for counter-examples. The procedure reduces the analysis of higher-order programs to checking satisfiability of a sequence of quantifier-free formulas over theories such as algebraic data types, integer linear arithmetic, and uninterpreted function symbols, thus enabling the use of efficient satisfiability modulo theory (SMT) solvers. Our solution supports arbitrary function types and arbitrarily nested anonymous functions (which can be stored in data structures, passed as arguments, returned, and applied). Among the contributions of this work is supporting even those cases when anonymous functions cannot be statically traced back to their definition, ensuring completeness of the approach for finding counter-examples. We provide a proof of soundness and counter-example completeness for our system as well as initial evaluation in the Leon verifier.
<title>Photodynamic activity of a number of photosensitizers in vitro</title> (1996)

The comparative study of the photosensitizers with different chemical, spectral-luminescent and photophysical properties (phthalocyanines -- photosense, di- and trisulphonated zinc (II) phthalocyanines; chlorines -- chlorine p6, desvinyl-3- formyl-chlorine p6 and 3-desvinyl-3-acetyl-chlorine p6; xanthenes --rhodamine 123, rhodamine 6G-chloride, rhodamine 6G-acetate, rhodamine 6G-iodide; arylamines -- oxazinne perchlorate, oxazine-1 zinc salt, 9-diethylamino-5- ethylaminobenzophe-nothiazonium acetate, methylene blue) was performed. The cyto- and the phototoxicity of these compounds were studied on the cells of two human tumor cell lines (Raji B-cell lymphoma and A-549 lung adenocarcinoma) by MTT-test. It was shown that phthalocyanines and chlorines were not cytotoxic, whereas xanthenes and arylamines possessed dark toxicity. On the basis of the IC<SUB>50</SUB> (the substance concentration, which induced 50% inhibition of cell proliferation in the cell culture) the rows of phototoxicity of the compounds of various classes were set out: in phthalocyanines: ZnPcS<SUB>2</SUB> greater than Photosence greater than ZnPcS<SUB>3</SUB>; in chlorines: 3dV-3F-Chl p6 greater than 3 dV-3Ac Chl p6 greater than Chl p6; in xanthenes: R6G- Ac greater than R6G-Cl greater than R6G-I greater than R 123; in arylamines: Meth B greater than or equal to 9-DE-B- Ac greater than Ox1-1/2ZnCl<SUB>4</SUB><SUP>2-</SUP> very much greater than Ox1-ClO<SUB>4</SUB><SUP>-</SUP>. Phototoxicity of the studied compounds depended on the nature of the substitutes and of the counter ions in photosensitizers molecules as well as on the concentration of the photosensitizer, on the light doses and on the regimes of irradiation. The fractionation of the light doses increased the efficiency of the phototoxic effect of the dyes on the tumor cells significantly. It was shown by the luminescent microscopy that the dynamics and the intensity of the accumulation of the rhodamines derivatives in lung adenocarcinoma cells depended on the nature of the counter ion in the photosensitizer molecule and correlated with their cyto- and pho-toxicity. Thus, photosense, di- and trisulphonated zinc phthalocyanines, 3- desvinyl-3-formyl-chlorine p6, rhodamine 6G-acetate and methylene blue turned out to be promising for their further study as photodynamic agents and efficient modifiers of chemoradiotherapy and of PDT.
A novel 10bit 90MS/s 2b/cycle SAR ADC (2013)

A 10-bit 90MS/s Successive Approximation Register (SAR) analog-to-digital converter (ADC) is realized in TSMC 0.18μm CMOS process. With 2b/cycle technique and new proposed asynchronous control logic, the proposed SAR ADC achieves rapid conversion rate, low power, leading to SNDR of 55.3 and SFDR of 64.7 at 90MS/s with 43M input. The active area with the digital calibration is 0.6×0.8mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
Telomerase reverse-transcriptase homozygous mutations in autosomal recessive dyskeratosis congenita and Hoyeraal-Hreidarsson syndrome (2007)

Dyskeratosis congenita (DC) is a multisystem bone marrow failure syndrome characterized by a triad of mucocutaneous abnormalities and an increased predisposition to malignancy. X-linked DC is due to mutations in DKC1, while heterozygous mutations in TERC (telomerase RNA component) and TERT (telomerase reverse transcriptase) have been found in autosomal dominant DC. Many patients with DC remain uncharacterized, particularly families displaying autosomal recessive (AR) inheritance. We have now identified novel homozygous TERT mutations in 2 unrelated consanguineous families, where the index cases presented with classical DC or the more severe variant, Hoyeraal-Hreidarsson (HH) syndrome. These TERT mutations resulted in reduced telomerase activity and extremely short telomeres. As these mutations are homozygous, these patients are predicted to have significantly reduced telomerase activity in vivo. Interestingly, in contrast to patients with heterozygous TERT mutations or hemizygous DKC1 mutations, these 2 homozygous TERT patients were observed to have higher-than-expected TERC levels compared with controls. Collectively, the findings from this study demonstrate that homozygous TERT mutations, resulting in a pure but severe telomerase deficiency, produce a phenotype of classical AR-DC and its severe variant, the HH syndrome.
A Role for SIRT2-Dependent Histone H3K18 Deacetylation in Bacterial Infection (2013)

Pathogens dramatically affect host cell transcription programs for their own profit during infection, but in most cases, the underlying mechanisms remain elusive. We found that during infection with the bacterium Listeria monocytogenes, the host deacetylase sirtuin 2 (SIRT2) translocates to the nucleus, in a manner dependent on the bacterial factor InlB. SIRT2 associates with the transcription start site of a subset of genes repressed during infection and deacetylates histone H3 on lysine 18 (H3K18). Infecting cells in which SIRT2 activity was blocked or using SIRT2(-/-) mice resulted in a significant impairment of bacterial infection. Thus, SIRT2-mediated H3K18 deacetylation plays a critical role during infection, which reveals an epigenetic mechanism imposed by a pathogenic bacterium to reprogram its host.
A convenient synthesis of methacrylates (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA convenient synthesis of methacrylatesAllen R. Banks, Richard F. Fibiger, and Ted JonesCite this: J. Org. Chem. 1977, 42, 24, 3965–3966Publication Date (Print):November 1, 1977Publication History Published online1 May 2002Published inissue 1 November 1977https://pubs.acs.org/doi/10.1021/jo00444a042https://doi.org/10.1021/jo00444a042research-articleACS PublicationsRequest reuse permissionsArticle Views1489Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Corrections of the NIST Statistical Test Suite for Randomness. (2004)

It is well known that the NIST statistical test suite was used for the evaluation of AES candidate algorithms. We have found that the test setting of Discrete Fourier Transform test and Lempel-Ziv test of this test suite are wrong. We give four corrections of mistakes in the test settings. This suggests that re-evaluation of the test results should be needed.
Electron paramagnetic resonance in boron carbide (2002)

Electron paramagnetic resonance (EPR) signals from powdered samples of boron carbide B4C are recorded at g factor 2.0028±0.0002. The dependence on temperature and thermal treatment of the samples is studied. We demonstrated that native defects of boron carbide and conduction electrons are responsible for EPR absorption. The temperature dependence of the EPR line width ΔB(T) is accounted for via the Korringa mechanism. By fitting experimental and theoretical curves for ΔB(T), distances of donor levels from the edge of the conduction band ΔEd are obtained. The process of sample preparation strongly effects the value of ΔEd. We assume a radical formation of the type B3C with local concentration Nloc∼1019–2×1020 cm−3 is responsible for the EPR signal under these conditions.
Identification of a novel function for 67-kDa laminin receptor: increase in laminin degradation rate and release of motility fragments. (2002)

The 67-kDa laminin receptor (67LR) is a high-affinity laminin-binding protein that is overexpressed on the tumor cell surface in a variety of cancers. We report here that the 67LR molecule also functions in the proteolytic cleavage of laminin-1, a relevant event in basement membrane degradation and tumor dissemination. In the presence of a synthetic peptide (peptide G) corresponding to the 67LR laminin binding site, the rate of laminin-1 degradation by the cysteine proteinase cathepsin B was significantly increased, and a new proteolytic fragment particularly active in in vitro cell migration assays was generated. The YIGSR peptide, corresponding to the 67LR binding site on laminin-1, blocked the peptide G-dependent proteolytic degradation. Our results shed light on the mechanism by which an adhesion receptor such as the 67LR plays a major role in tumor aggressiveness and metastasis.
Multifaceted roles of extracellular DNA in bacterial physiology (2015)

In textbooks, DNA is generally defined as the universal storage material for genetic information in all branches of life. Beyond this important intracellular role, DNA can also be present outside of living cells and is an abundant biopolymer in aquatic and terrestrial ecosystems. The origin of extracellular DNA in such ecological niches is diverse: it can be actively secreted or released by prokaryotic and eukaryotic cells by means of autolysis, apoptosis, necrosis, bacterial secretion systems or found in association with extracellular bacterial membrane vesicles. Especially for bacteria, extracellular DNA represents a significant and convenient element that can be enzymatically modulated and utilized for multiple purposes. Herein, we discuss briefly the main origins of extracellular DNA and the most relevant roles for the bacterial physiology, such as biofilm formation, nutrient source, antimicrobial means and horizontal gene transfer.
Resonant free power network design using extended adaptive voltage positioning (EAVP) methodology (2001)

Extended adaptive voltage positioning (EAVP) is a new robust methodology for the design and analysis of a low impedance resonant free power delivery network, which utilizes and extends the theory of adaptive voltage positioning (AVP) that is commonly used in voltage regulator module (VRM) design and operation. Using EAVP, uncertainties in design guardband noise budget can be removed, resulting in significant performance bin-split improvement and cost reduction. Design optimization of decoupling capacitors with EAVP will be illustrated by using both time and frequency domain analysis.
Advances in the pathophysiology of status epilepticus (2007)

Status epilepticus (SE) describes an enduring epileptic state during which seizures are unremitting and tend to be self-perpetuating. We describe the clinical phases of generalized convulsive SE, impending SE, established SE, and subtle SE. We discuss the physiological and biochemical cascades which characterize self-sustaining SE (SSSE) in animal models. At the transition from single seizures to SSSE, GABA(A) (gamma-aminobutyric acid) receptors move from the synaptic membrane to the cytoplasm, where they are functionally inactive. This reduces the number of GABA(A) receptors available for binding GABA or GABAergic drugs, and may in part explain the development of time-dependent pharmacoresistance to benzodiazepines and the tendency of seizures to become self-sustaining. At the same time, 'spare' subunits of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) and NMDA (N-methyl-D-aspartic acid) receptors move from subsynaptic sites to the synaptic membrane, causing further hyperexcitability and possibly explaining the preserved sensitivity to NMDA blockers late in the course of SE. Maladaptive changes in neuropeptide expression occur on a slower time course, with depletion of the inhibitory peptides dynorphin, galanin, somatostatin and neuropeptide Y, and with an increased expression of the proconvulsant tachykinins, substance P and neurokinin B. Finally, SE-induced neuronal injury and epileptogenesis are briefly discussed.