gebhart/patrepeval huggingface dataset:
POISON RETENTION IN REACTOR FUSES (1957)

Recent inquiries have revealed a surprisingly large number (>2500) of naturally occurring antisense transcripts, but their function remains largely undiscovered. A better understanding of antisense mechanisms is clearly needed because of their potentially diverse roles in gene regulation and disease. A well-documented case occurs in X inactivation, the mechanism by which X-linked gene expression is equalized between XX females and XY males. The antisense gene Tsix determines X chromosome choice and represses the noncoding silencer, Xist. In principle, Tsix action may involve RNA, the act of transcription, or local chromatin. Here, we create novel Tsix alleles to distinguish transcription- versus RNA-based mechanisms. When Tsix transcription is terminated before Xist (TsixTRAP), Tsix cannot block Xist upregulation, suggesting the importance of overlapping antisense transcription. To separate the act of transcription from RNA, we knocked in Tsix cDNA in the reverse orientation (Tsix(cDNA)) to restore RNA levels in cis without concurrent transcription across Xist. However, Tsix(cDNA) cannot complement TsixTRAP. Surprisingly, both mutations disrupt choice, indicating that this epigenetic step requires transcription. We conclude that the processed antisense RNA does not act alone and that Tsix function specifically requires antiparallel transcription through Xist. A mechanism of transcription-based feedback regulation is proposed.
<title>Multiview 3D LCD</title> (1996)

The combination of lenticular screens with liquid crystal displays has long been recognized as an excellent way of making autostereoscopic displays. Now, as active matrix video rate displays with full color VGA and higher resolution are becoming readily available, interest in this form of autostereoscopic imaging is increasing. An important consequence of the high resolution capability is that the `resolution/number of views' trade-off can now be made in more in favor of the `number of views' instead of the traditionally favored `resolution' side of the equation. This means that head tracking is no longer the only option for providing a more natural viewing environment. The Philips group is a manufacturer of both Active Matrix LCDs and lenticular screens. We have been able to experiment with multiview displays by combining customized lenticular screens with special liquid crystal modules. In particular, a monochrome display with four views, in which each view has a 480 X 480 resolution, has been made. Compared with two view systems in which the user has to maintain a fixed head position, the extra freedom afforded by the four views is experienced as a great improvement and most people have little difficulty avoiding the one remaining pseudoscopic head position. These multiview displays are receiving an enthusiastic response in applications areas such as medical radio diagnostics and multimedia entertainment.
Eag1 Gene and Protein Expression in Human Retinoblastoma Tumors and its Regulation by pRb in HeLa Cells (2020)

Retinoblastoma is the most common pediatric intraocular malignant tumor. Unfortunately, low cure rates and low life expectancy are observed in low-income countries. Thus, alternative therapies are needed for patients who do not respond to current treatments or those with advanced cases of the disease. <i>Ether à-go-go-1</i> (Eag1) is a voltage-gated potassium channel involved in cancer. <i>Eag1</i> expression is upregulated by the human papilloma virus (HPV) oncogene E7, suggesting that retinoblastoma protein (pRb) may regulate <i>Eag1</i>. Astemizole is an antihistamine that is suggested to be repurposed for cancer treatment; it targets proteins implicated in cancer, including histamine receptors, ATP binding cassette transporters, and Eag channels. Here, we investigated Eag1 regulation using pRb and Eag1 expression in human retinoblastoma. The effect of astemizole on the cell proliferation of primary human retinoblastoma cultures was also studied. HeLa cervical cancer cells (HPV-positive and expressing Eag1) were transfected with <i>RB1</i>. <i>Eag1</i> mRNA expression was studied using qPCR, and protein expression was assessed using western blotting and immunochemistry. Cell proliferation was evaluated with an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. <i>RB1</i> transfection down-regulated Eag1 mRNA and protein expression. The human retinoblastoma samples displayed heterogeneous Eag1 mRNA and protein expression. Astemizole decreased cell proliferation in primary retinoblastoma cultures. Our results suggest that Eag1 mRNA and protein expression was regulated by pRb in vitro, and that human retinoblastoma tissues had heterogeneous Eag1 mRNA and protein expression. Furthermore, our results propose that the multitarget drug astemizole may have clinical relevance in patients with retinoblastoma, for instance, in those who do not respond to current treatments.
Research progress on cancer-related fatigue (2012)

Fatigue is one of the most common complaints in cancer patients that profoundly affects all aspects of quality of life.However,cancer-related fatigue remains under recognized and poorly treated.This rcview has been conducted with the causes and potential treatments of fatigue in cancer patients.The methods for evaluating fatigue,and possible therapeutic options are also discussed.

Key words: 
Neoplasms;  Mental fatigue;  Pathogenesis;  Evaluation;  Treatment
Comparisons of sequence labeling algorithms and extensions (2007)

In this paper, we survey the current state-of-art models for structured learning problems, including Hidden Markov Model (HMM), Conditional Random Fields (CRF), Averaged Perceptron (AP), Structured SVMs (SVMstruct), Max Margin Markov Networks (M3N), and an integration of search and learning algorithm (SEARN). With all due tuning efforts of various parameters of each model, on the data sets we have applied the models to, we found that SVMstruct enjoys better performance compared with the others. In addition, we also propose a new method which we call the Structured Learning Ensemble (SLE) to combine these structured learning models. Empirical results show that our SLE algorithm provides more accurate solutions compared with the best results of the individual models.
Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism. Induction of de novo synthesis of prostaglandin H synthase. (1988)

We studied the effects of natural and recombinant human IL-2 (rIL-2) on secretion of prostacyclin (PGI2), vWf, and tissue-type plasminogen activator (tPA). IL-2 elicited a steady increase in PGI2 synthesis by cultured human umbilical vein endothelial cells (HUVECS) and bovine aortic endothelial cells but had no effect on vWf or tPA. Both purified natural IL-2 (nIL-2) and rIL-2 induced significant PGI2 synthesis. Substitution of the cysteine residue at position 125 of rIL-2 with serine or alanine led to loss of PGI2-stimulatory activity in HUVECS without affecting thymidine incorporation in lymphocytes. HPLC analysis of arachidonate metabolites detected predominantly 6 keto-PGF1 alpha (6KPGF1 alpha) peak. Treatment of cultured endothelial cells with cycloheximide and actinomycin D resulted in inhibition of 6KPGF1 alpha synthesis. The Western blot using a polyclonal antibody against PGH synthase revealed an increment in the 70-kD subunit of PGH synthase by nIL-2 and rIL-2, but not by alanine-substituted rIL-2. We conclude that IL-2 stimulated sustained PGI2 production by a mechanism that includes the de novo synthesis of PGH synthase. This mechanism for regulating AA metabolism probably has important physiologic implications.
Computation of coastal sea water absorption coefficients and retrieval of water quality parameters from EO1 Hyperion data (2003)

This work aims to extract optical parameters of coastal sea water by fitting the hyperspectral reflectance spectra acquired from the Hyperion instrument on-board the EO-1 satellite to a coupled sea water reflectance and atmospheric transmission model. This method is able to retrieve the absorption and scattering coefficients of the various components of coastal sea water.
Characterization of partially transesterified poly(β-hydroxyalkanoate)s using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (1999)

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was used for the characterization of a partially transesterified poly(beta-hydroxyalkanoate), PHA, polymer produced by the bacterial strain Alcaligenes eutrophus using saponified vegetable oils as the sole carbon sources. The transesterification was carried out separately under acidic and basic conditions to obtain PHA oligomers weighing less than 10 kDa. The intact oligomers were detected in their cationized [M + Na](+) and [M + K](+) forms by MALDI-TOFMS. A composition analysis, using the MALDI-TOF spectra, indicate that the oligomers obtained via acid catalysis were terminated with a methyl 3-hydroxybutyrate end group, and those obtained by base catalysis had a methyl crotonate (olefinic) termination. In addition to HB (hydroxy butyrate), the oligomers were found to contain a small percentage of HV (hydroxy valerate). This was independently confirmed using gas chromatography/mass spectrometry (GC/MS). In comparison, the analysis of a commercial PHA polymer, transesterified under identical conditions, only showed the presence of HB, i.e. a pure PHB homopolymer. Copyright 1999 John Wiley & Sons, Ltd.
RAPD and SCAR Markers Linked to an Introgressed Gene Conditioning Resistance to <i>Peronospora tabacina</i> D.B. Adam. in Tobacco (2005)

Blue mold, caused by the fungal pathogen Peronospora tabacina D.B. Adam, is one of the most important foliar diseases of tobacco ( Nicotiana tabacum L.). Identification of molecular markers linked to genetic factors controlling resistance would facilitate development of resistant cultivars. Bulked segregant analysis was used to screen 1216 random amplified polymorphic DNA (RAPD) primers for their ability to reveal polymorphism between DNA bulks from susceptible doubled haploid (DH) lines and resistant DH lines possessing resistance derived from cultivar Ovens 62. Fifteen RAPD markers were tentatively identified as being linked to a major gene conditioning resistance to blue mold. These 15 markers (12 in coupling phase linkage with resistance and three in repulsion phase) were found to lie within a single linkage group of 36.6 cM and were subsequently tested on 122 DH lines derived from crosses between resistant and susceptible parents. F tests revealed statistically significant associations between resistance and each of the 15 RAPD markers. Interval mapping was used to more accurately place the quantitative trait locus (QTL) controlling resistance on the linkage map. The RAPD markers were screened on a set of 45 resistant and susceptible cultivars or breeding lines and four Nicotiana species. At variance with previous reports, marker genotypes indicated that resistance in Ovens 62 and most other blue mold resistant lines likely originated from N. debneyi Domin. Two RAPD markers flanking the most likely QTL position were converted to sequence characterized amplified region (SCAR) markers. These markers should aid in development of blue mold‐resistant tobacco cultivars worldwide.
Antibacterial Activity and Uptake into Escherichia coli of Backbone-modified Analogues of Small Peptides (1983)

Analogues of di- and tripeptides in which the peptide backbone is modified have been examined for antibacterial activity in vitro, and for uptake into Escherichia coli. Aminoxy and hydrazino types, in which the peptide linkage is replaced, respectively, by -CO-NHO- or -CO-NH-NH-, were active against E. coli, Staphylococcus aureus, and Salmonella dublin; retro, alpha-aza, tetrazole, and hydroxamic types were inactive. Highest potency against all three species was found in aminoxy analogues containing D-2-aminoxypropionic acid (D-OAla) residues, Ala-D-OAla being active at less than 1 mg 1-1. Uptake into E. coli was seen with all active types, but, with the exception of hydroxamic analogues not with the inactive types. Following uptake the toxic analogues were rapidly hydrolysed and the constituent amino acid residues underwent exodus. The substrate specificities of the peptide transport systems have been further defined on the basis of our results.
Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation/hydrolysis ratio (2008)

The reuteransucrase enzymes of Lactobacillus reuteri strain 121 (GTFA) and L. reuteri strain ATCC 55730 (GTFO) convert sucrose into alpha-d-glucans (labelled reuterans) with mainly alpha-(1-->4) glucosidic linkages (50% and 70%, respectively), plus alpha-(1-->6) linkages. In the present study, we report a detailed analysis of various hybrid GTFA/O enzymes, resulting in the identification of specific regions in the N-termini of the catalytic domains of these proteins as the main determinants of glucosidic linkage specificity. These regions were divided into three equal parts (A1-3; O1-3), and used to construct six additional GTFA/O hybrids. All hybrid enzymes were able to synthesize alpha-glucans from sucrose, and oligosaccharides from sucrose plus maltose or isomaltose as acceptor substrates. Interestingly, not only the A2/O2 regions, with the three catalytic residues, affect glucosidic linkage specificity, but also the upstream A1/O1 regions make a strong contribution. Some GTFO derived hybrid/mutant enzymes displayed strongly increased transglucosylation/hydrolysis activity ratios. The reduced sucrose hydrolysis allowed the much improved conversion of sucrose into oligo- and polysaccharide products. Thus, the glucosidic linkage specificity and transglucosylation/hydrolysis ratios of reuteransucrase enzymes can be manipulated in a relatively simple manner. This engineering approach has yielded clear changes in oligosaccharide product profiles, as well as a range of novel reuteran products differing in alpha-(1-->4) and alpha-(1-->6) linkage ratios.
A New Current-Sensing Device (1963)

Development of a reliable, low-cost current sensing device was undertaken for use in capacitor switching, load study, and protective relaying. The unit takes the form of an inductive pick-up coil imbedded in a porcelain insulator. It may be used on straight conductor runs with an accuracy of ±6% before applying correction factors which depend on physical configuration and size of conductor. Output impedance is high, suggesting use of high-impedance loads. Sufficient power is available to operate electronic equipment and sensitive relays.
Magnetostatic wave interdigital transducers (1978)

The design and operation of a novel type of magnetostatic wave transducer is described. The transducer is formed from an interdigital arrangement of narrow, open circuited microstrip fingers, each half an electromagnetic wavelength long. Experimental results in the frequency range 9–9.4 GHz are presented for 2, 4 and 10 finger transducers which are in good agreement with the sin(Nx/2)/sin(x/2) response predicted by simple theory.
Structure of a phonological rule component for a synthesis-by-rule program (1976)

A synthesis-by-rule program can be thought of as a functional model of human sentence production. The phonological component of this model accepts as input a linear string of symbols that have been produced by the semantics component, syntactic component, and lexical component of a grammar of English. This abstract representation for an utterance is transformed by the phonological component into a narrow phonetic transcription and a specification of stress levels, segmental durations, and aspects of the fundamental frequency contour.
In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis (1991)

In order to examine potential regulatory steps in plant fatty acid biosynthesis, we have developed procedures for the analysis of the major acyl-acyl carrier protein (ACP) intermediates of this pathway. These techniques have been used to separate and identify acyl-ACPs with chain configurations ranging from 2:0 to 18:1 and to determine the relative in vivo concentrations of acyl-ACPs in spinach leaf and developing seed. In both leaf and seed as much as 60% of the total ACPs were nonesterified (free), with the remaining proportion consisting of acyl-ACP intermediates leading to the formation of palmitate, stearate, and oleate. In spinach leaf the proportions of the various acyl groups esterified to each ACP isoform were indistinguishable, indicating that these isoforms are utilized similarly in de novo fatty acid biosynthesis in vivo. However, the acyl group distribution pattern of seed ACP-II differed significantly from that of leaf ACP-II. The malonyl-ACP levels were less than the 4:0-ACP and 6:0-ACP levels in leaf, and in contrast, the malonyl-ACP-II levels in seed were approximately 3-fold higher than the 4:0-ACP-II and 6:0-ACP-II levels. In addition, the ratio of oleoyl-ACP-II (18:1) to stearoyl-ACP-II (18:0) was higher in seed than in leaf. These data suggest that the differences in acyl-ACP patterns reflect a tissue/organ-specific difference rather than an isoform-specific difference. In extracts prepared from leaf samples collected in the dark, the levels of acetyl-ACPs were approximately 5-fold higher compared to samples collected in the light. The levels of free ACPs showed an inverse response, increasing in the light and decreasing in the dark. Notably there was no concomitant increase in the malonyl-ACP levels. The most likely explanation for the major increase in acetyl-ACP levels in the dark is that light/dark control over the rate of fatty acid biosynthesis occurs at the reaction catalyzed by acetyl-CoA carboxylase.
A Simple Fiber-Optic Flowmeter Based on Bending Loss (2009)

The characteristics of a macrobending fiber used as an optical flowmeter are discussed. When a liquid flows through the bending area of the fiber sensor, it will result to the variation of the bending curvature and modulate the bending loss of the fiber, due to the impact or/and the drawing force of the flow which is relative to the speed of the flow. Different curvatures in initial state have different performances in the measurement. An optimum performance of the curvature radius of 8 mm in the range of flow rate from 0 to 3 m/s is found in the experiment. A block hung up the bending fiber to enhance the sensitivity and the dynamic range is demonstrated. Twenty times larger of the loss change is achieved by hanging an 11 times 11 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> block. The experiment shows the influences of temperature and hysteresis can be ignored.
Room-Temperature Conversion of Soybean Oil and Poultry Fat to Biodiesel Catalyzed by Nanocrystalline Calcium Oxides (2006)

A promising route for the production of biodiesel (fatty acid methyl esters, FAMES) via transesterification of soybean oil (SBO) and poultry fat with methanol in quantitative conversions at room temperature has been developed using nanocrystalline calcium oxides as catalysts. Under the same conditions, laboratory-grade CaO gave only 2% conversion in the case of SBO, and there was no observable reaction with poultry fat. The soybean oil/methanol ratio in our protocol is 1:27. With our most active catalyst, deactivation was observed after eight cycles with SBO and after three cycles with poultry fat. Deactivation may be associated with one or more of the following factors: the presence of organic impurities or adventitious moisture and enolate formation by the deprotonation of the carbon alpha to the carboxy group in the triglyceride or FAMES. The biodiesel from our protocol meets the ASTM D-874 standard for sulfated ash for both substrates.
Characterisation of the metal‐ion–GDP complex at the active sites of transforming and nontransforming p21 proteins by observation of the <sup>17</sup>O‐Mn superhyperfine coupling and by kinetic methods (1987)

Kinetic studies on the interaction of three Ha-ras-encoded p21 proteins with GDP and MgGDP have yielded values for the association (10(6)-10(7) M-1 s-1) and dissociation (10(-3)-10(-5) s-1) rate constants at 0 degrees C. Dramatic differences in the rate constants were not observed for the three proteins. Under non-physiological conditions (absence of Mg2+), the rate constant for GDP release was an order of magnitude faster for the viral protein p21v than for the cellular form p21c or the T24 mutant p21t, but this was reduced to a factor of about 3 in the presence of Mg2+. In all cases, there was an increase of about one order of magnitude in the rate of GDP release on removing magnesium. The binding affinities ranged from 5.7 X 10(10) M-1 for p21c to 1.3 X 10(11) M-1 for p21v. Electron paramagnetic resonance (EPR) measurements on Mn2+ bound together with stereospecifically 17O-labelled GDP showed direct coordination of a beta-phosphate oxygen to the metal ion with a superhyperfine coupling constant of 0.16-0.22 mT, but no interaction with the alpha-phosphate oxygens at the active site of all three proteins. The association constant of Mn(II) to p21 proteins in the absence of nucleotides was estimated to be greater than 10(5) M-1. In agreement with the EPR results, experiments on the metal ion dependence of the binding of thiophosphate analogs of GDP provided further evidence for the absence of direct coordination of the metal ion to the alpha-phosphate group. These results have been used to construct a model for the interactions of Mg X GDP with the active site of p21 proteins.
Spin-Decoupled Projectiles: Relationship of Spin to Muzzle Velocity (1976)

A rationale is presented to explain the decrease in spin rate which frequently accompanies an increase in muzzle velocity for gun projectiles fitted with plastic sealing bands.
Domain Knowledge Uncertainty and Probabilistic Parameter Constraints (2012)

Incorporating domain knowledge into the modeling process is an effective way to improve learning accuracy. However, as it is provided by humans, domain knowledge can only be specified with some degree of uncertainty. We propose to explicitly model such uncertainty through probabilistic constraints over the parameter space. In contrast to hard parameter constraints, our approach is effective also when the domain knowledge is inaccurate and generally results in superior modeling accuracy. We focus on generative and conditional modeling where the parameters are assigned a Dirichlet or Gaussian prior and demonstrate the framework with experiments on both synthetic and real-world data.
A fast motion estimation algorithm based on the block sum pyramid (1997)

In this correspondence, a fast approach to motion estimation is presented. The algorithm uses the block sum pyramid to eliminate unnecessary search positions. It first constructs the sum pyramid structure of a block. Successive elimination is then performed hierarchically from the top level to the bottom level of the pyramid. Many search positions can be skipped from being considered as the best motion vector and, thus, the search complexity can be reduced. The algorithm can achieve the same estimation accuracy as the full search block matching algorithm with much less computation time.
Continuous Topical Administration of a Petrolatum Formulation by a Novel Disposable Diaper (2000)

The results demonstrated the clinical benefits associated with continuous topical administration of a petrolatum-based formulation by this novel diaper. We anticipate that this advance in diaper design will contribute significantly to further reduce the prevalence and severity of irritant contact dermatitis in the diaper area.
A lysine walk to high relaxivity collagen-targeted MRI contrast agents (2008)

A strategy for preparing peptide-based magnetic resonance contrast agents with multiple gadolinium chelates is described.