gebhart/patrepeval huggingface dataset:
Identification and Functional Characterization of a Novel Nuclear Localization Signal Present in the Yeast Nab2 Poly(A) <sup>+</sup> RNA Binding Protein (1998)

The nuclear import of proteins bearing a basic nuclear localization signal (NLS) is dependent on karyopherin alpha/importin alpha, which acts as the NLS receptor, and karyopherin beta1/importin beta, which binds karyopherin alpha and mediates the nuclear import of the resultant ternary complex. Recently, a second nuclear import pathway that allows the rapid reentry into the nucleus of proteins that participate in the nuclear export of mature mRNAs has been identified. In mammalian cells, a single NLS specific for this alternate pathway, the M9 NLS of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1), has been described. The M9 NLS binds a transport factor related to karyopherin beta1, termed karyopherin beta2 or transportin, and does not require a karyopherin alpha-like adapter protein. A yeast homolog of karyopherin beta2, termed Kap104p, has also been described and proposed to play a role in the nuclear import of a yeast hnRNP-like protein termed Nab2p. Here, we define a Nab2p sequence that binds to Kap104p and that functions as an NLS in both human and yeast cells despite lacking any evident similarity to basic or M9 NLSs. Using an in vitro nuclear import assay, we demonstrate that Kap104p can direct the import into isolated human cell nuclei of a substrate containing a wild-type, but not a defective mutant, Nab2p NLS. In contrast, other NLSs, including the M9 NLS, could not function as substrates for Kap104p. Surprisingly, this in vitro assay also revealed that human karyopherin beta1, but not the Kap104p homolog karyopherin beta2, could direct the efficient nuclear import of a Nab2p NLS substrate in vitro in the absence of karyopherin alpha. These data therefore identify a novel NLS sequence, active in both yeast and mammalian cells, that is functionally distinct from both basic and M9 NLS sequences.
Performance analysis for collaborative decoding with least-reliable-bits exchange on AWGN channels (2008)

Collaborative decoding is an approach that can achieve diversity and combining gain by exchanging decoding information among a cluster of physically separated receivers. On AWGN channels, the least-reliable-bits (LRB) exchange scheme can achieve performance close to equal-gain combining (EGC) of all received symbols from all receivers, while reducing the amount of information that must be exchanged. In this paper, we analyze the error performance of collaborative decoding with the LRB exchange scheme when nonrecursive convolutional codes are used. The analysis is based on the observation that the extrinsic information generated in the collaborative decoding of these convolutional codes can be approximated by Gaussian random variables. A density-evolution model based on a single maximum a posteriori decoder is used to obtain the statistical characteristics of the extrinsic information. With the statistical knowledge of the extrinsic information, we develop an approximate upper bound for the error performance of the collaborative decoding process. Numerical results show that our analysis gives very good predictions of the bit error rate for collaborative decoding with LRB exchange. At high signal-to-noise ratios collaborative decoding with properly chosen parameters can achieve the same error performance as EGC of all received symbols from all receiving nodes.
Tunnel Sprayer for Dwarf Fruit Trees (1994)

A tunnel sprayer was developed for dwarf fruit trees. Spray deposit studies with various delivery devices revealed that the most uniform deposition within the tree and on both leaf surfaces was achieved by using two pairs of opposing cross-flow fans with hydraulic nozzles. The tunnel sprayer showed significant drift reduction potential over a commercial air-blast sprayer.
γ-Alumina: a single-crystal X-ray diffraction study (2006)

The structure of gamma-alumina (Al21+1/3square2+2/3O32) crystals obtained as a product of a corrosion reaction between beta-sialon and steel was refined in the space group Fd3m. The oxygen sublattice is fully occupied. The refined occupancy parameters are 0.83 (3), 0.818 (13), 0.066 (14) and 0.044 (18) for Al ions in 8a, 16d, 16c and 48f positions, respectively. The Al ions are distributed over octahedral and tetrahedral sites in a 63:37 ratio, with 6% of all Al ions occupying non-spinel positions.
Cross-lingual portability of Chinese and english neural network features for French and German LVCSR (2011)

This paper investigates neural network (NN) based cross-lingual probabilistic features. Earlier work reports that intra-lingual features consistently outperform the corresponding cross-lingual features. We show that this may not generalize. Depending on the complexity of the NN features, cross-lingual features reduce the resources used for training -the NN has to be trained on one language only- without any loss in performance w.r.t. word error rate (WER). To further investigate this inconsistency concerning intra- vs. cross-lingual neural network features, we analyze the performance of these features w.r.t. the degree of kinship between training and testing language, and the amount of training data used. Whenever the same amount of data is used for NN training, a close relationship between training and testing language is required to achieve similar results. By increasing the training data the relationship becomes less, as well as changing the topology of the NN to the bottle neck structure. Moreover, cross-lingual features trained on English or Chinese improve the best intra-lingual system for German up to 2% relative in WER and up to 3% relative for French and achieve the same improvement as for discriminative training. Moreover, we gain again up to 8% relative in WER by combining intra- and cross-lingual systems.
Human Dental Age Estimation by Calculation of Pulp–Tooth Volume Ratios Yielded on Clinically Acquired Cone Beam Computed Tomography Images of Monoradicular Teeth* (2010)

Secondary dentine is responsible for a decrease in the volume of the dental pulp cavity with aging. The aim of this study is to evaluate a human dental age estimation method based on the ratio between the volume of the pulp and the volume of its corresponding tooth, calculated on clinically taken cone beam computed tomography (CBCT) images from monoradicular teeth. On the 3D images of 111 clinically obtained CBCT images (Scanora(®) 3D dental cone beam unit) of 57 female and 54 male patients ranging in age between 10 and 65 years, the pulp-tooth volume ratio of 64 incisors, 32 canines, and 15 premolars was calculated with Simplant(®) Pro software. A linear regression model was fit with age as dependent variable and ratio as predictor, allowing for interactions of specific gender or tooth type. The obtained pulp-tooth volume ratios were the strongest related to age on incisors.
SPECIAL SECTION: Medication Instruction Design: Younger and Older Adult Schemas for Taking Medication (1996)

We examined whether older and younger adults share a schema for taking medication and whether instructions are better recalled when they are organized to match this schema. Experiment 1 examined age difference in schema organization. Participants sorted medication items (e.g., purpose, dose, possible side effects) according to similarity and then ordered the items to create a preferred instruction set. Cluster analysis of the sort and order data showed that younger and older adults share a schema for taking medication. Secondary regression analyses found that verbal ability (i.e., vocabulary scores) predicted individual differences in schema organization. In Experiment 2 participants recalled instructions that were either compatible with this schema in terms of grouping and order of items or were presented in nonpreferred orders. Younger participants remembered more information than did older participants, but both age groups better remembered and preferred the more schema-compatible instructions. Secondary analyses showed that recall was also positively related to verbal ability. Along with our earlier research, this study suggests that older and younger adults possess a schema for taking medication and that instructions that are compatible with this schema provide an environmental support that improves memory for medication information.
Soft Magnetic Properties of Fe-N Films Composed of Two Phases (1994)

Fe-N films were synthesized by means of ion beam deposition. Nitridation was performed using an assist gun during the sputtering of Fe films. The as-deposited Fe-N films consisted of two phases, μ-Fe and γ'-Fe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> N, and had a high coercive force H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> of over 20 Oe. When the films were annealed in vacuum, their H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> improved drastically, and had a minimum value of less than 1 Oe at 400°C. The films also had a high permeability of over 2000 as well as high resistivity (around 80 μΩ-cm), in spite of a high saturation magnetization (around 18 kG). The small grain size and stress relaxation in the films are closely related to the soft magnetic properties. The volume fraction of the γ'-Fe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> N phase also affected the decrease in H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> , because the anisotropy normal to the film plane is reduced by increasing the magnetostatic energy when the volume fraction was 0.5.
Sequence-selective depurination, DNA interstrand cross-linking and DNA strand break formation associated with alkylated DNA (1992)

Alkylation of DNA by chloroethylnitrosourea (CNU) at the guanine N7 position has been shown to occur in a sequence-selective fashion. In this report we find that the depurination of these alkylated sites occurs with two distinct kinetic components--GG sequences depurinate within 30 min of exposure to CNU, while depurination at GT sequences is first observed after 1 h and continues to increase 16 h after drug exposure. These apurinic sites are converted to DNA strand breaks and constitute less than 10% of the total sites of guanine N7 alkylation. Spermidine was found to decrease alkylation in 5'-GG-3' sequences but increases alkylation at 5'-GTC-3' sequences. These findings suggest that the majority of the guanine N7 alkylations formed by CNU are stable, with a minor adduct being responsible for the slow depurination event. We propose that the rapid depurination induced by CNU occurs from an initial guanine O6 alkylation, which then depurinates via a guanine O6-N7 cyclized intermediate. We also propose that the resulting apurinic sites may lead to DNA interstrand cross-linking (ISC). In support of these hypotheses we show that (i) DNA modified with the monoalkylating agent dimethylsulfate forms DNA ISC upon depurination; (ii) ellagic acid enhances the level of guanine N7 alkylation and alters the pattern of sequence selectivity shown by three bifunctional chloroethylating agents CNU, mitozolomide and methyl 3-(2-chloroethyl)-4-oxoimidazo[5,1-d]-1,2,3,5-tetrazine-8-ca rboxylate but not with nitrogen mustard; (iii) ellagic acid has no effect upon the frequency of alkylation observed with the monofunctional alkylators N-methyl-N-nitrosourea, N-ethyl-N-nitrosourea and methylmethanesulfonate; (iv) ellagic acid increases the frequency of depurination and strand break formation induced by CNU without affecting the sequence-selective pattern of depurination.
SEAPORT: Assessing the Portability of Serverless Applications (2020)

\n The term serverless is often used to describe cloud applications\n that comprise components managed by third parties. Like any other\n cloud application, serverless applications are often tightly-coupled\n with providers, their features, models, and APIs. As a result, when\n their portability to another provider has to be assessed,\n application owners must deal with identification of heterogeneous\n lock-in issues and provider-specific technical details.\n Unfortunately, this process is tedious, error-prone, and requires\n significant technical expertise in the domains of serverless and\n cloud computing. In this work, we introduce SEAPORT, a method for\n automatically assessing the portability of serverless applications\n with respect to a chosen target provider or platform. The method\n introduces (i) a canonical serverless application model, and (ii)\n the concepts for portability assessment involving classification and\n components similarity calculation together with the static code\n analysis. The method aims to be compatible with existing migration\n concepts to allow using it as a complementary part for serverless\n use cases. We present an architecture of a decision support system\n supporting automated assessment of the given application model with\n respect to the target provider. To validate the technical\n feasibility of the method, we implement the system prototypically.\n
Purification and properties of Bacillus subtilis SA-22 endo-1, 4-beta-D-mannanase. (2003)

beta-mannanase (EC 3.2.1.78) from Bacillus subtilis SA-22 was purified successively by ammonium sulfate precipitation, hydroxyapatite chromatography, Sephadex G-75 gel filtration and DEAE-52 anion-exchange chromatography. Through these steps, the enzyme was concentrated 30.75-fold with a recovery rate of 23.43%, with a specific activity of 34780.56 u/mg. Molecular weight of the enzyme was determined to be 38 kD by SDS-PAGE and 34 kD by gel filtration. The results revealed that the optimal pH value for the enzyme was 6.5 and the optimal temperature was 70 degrees C. The enzyme is stable between pH 5 to 10. The enzyme remained most of its activity after a treatment of 4 h at 50 degrees C, but lost 25% of activity at 60 degrees C for 4 h, lost 50% of activity at 70 degrees C for 3 h. The enzyme activity was strongly inhibited by Hg2+. The Michaelis constants (Km) were measured as 11.30 mg/mL for locust bean gum and 4.76 mg/mL for konjac powder, while Vmax for these two polysaccharides were 188.68 (micromol x mL(-1) x min(-1)) and 114.94 (micromol x mL(-1) x min(-1)), respectively.
Green Polyurethane-- A new use for a low-value biodiesel byproduct (2011)

Since they were first developed in 1937, polyurethanes have become some of the most versatile polymers in the world. Flexible and rigid foams are the most common applications, and these polyurethane foams can be found in the automotive, construction, and insulation industries, among others. Unfortunately, the polyurethane industry is heavily petroleum-dependent because the two major raw materials (i.e., polyols and isocyantes) are largely petroleum-derived. To combat concerns over the depletion of global petroleum reserves and rising petroleum prices, extensive research has been conducted to produce biobased polyols (biopolyols) from renewable sources to replace petroleum-based polyols.
Do all birds tweet the same? (2011)

Social media services have spread throughout the world in just a few years. They have become not only a new source of information, but also new mechanisms for societies world-wide to organize themselves and communicate. Therefore, social media has a very strong impact in many aspects -- at personal level, in business, and in politics, among many others. In spite of its fast adoption, little is known about social media usage in different countries, and whether patterns of behavior remain the same or not. To provide deep understanding of differences between countries can be useful in many ways, e.g.: to improve the design of social media systems (which features work best for which country?), and influence marketing and political campaigns. Moreover, this type of analysis can provide relevant insight into how societies might differ. In this paper we present a summary of a large-scale analysis of Twitter for an extended period of time. We analyze in detail various aspects of social media for the ten countries we identified as most active. We collected one year's worth of data and report differences and similarities in terms of activity, sentiment, use of languages, and network structure. To the best of our knowledge, this is the first on-line social network study of such characteristics.
Solution-processed 6,13-bis(triisopropylsilylethynyl) (TIPS) pentacene thin-film transistors with a polymer dielectric on a flexible substrate (2008)

The authors report the fabrication of solution-processed 6,13-bis(triisopropylsilylethynyl) (TIPS) pentacene thin-film transistors with a cross-linked poly-4-vinylphenol (PVP) dielectric on a polyethersulphone (PES) substrate. The device exhibited useful electrical characteristics, including a saturation field effect mobility of 2.08 × 10−2 cm2 V−1 s−1, a current on/off ratio of 105, a threshold voltage of −2 V and an excellent subthreshold slope of 0.86 V/dec. It was demonstrated that the significant improvement in the subthreshold slope of TIPS-pentacene TFTs could be attributed to a decreased carrier trap density at the PVP/TIPS-pentacene film interface. Furthermore, a 1,2,3,4-tetrahydronaphthalene (Tetralin) solvent used in this study had a high boiling point, which had a positive effect on the morphology and the molecular ordering of the TIPS-pentacene film.
Chemically Synthesized SDF-1α Analogue, N33A, Is a Potent Chemotactic Agent for CXCR4/Fusin/LESTR-expressing Human Leukocytes (1997)

Stromal cell-derived factor (SDF) 1 is a potent chemoattractant for leukocytes through activation of the receptor CXCR4/Fusin/LESTR, which is a fusion co-factor for the entry of T lymphocytotropic human immunodeficiency virus type 1 (HIV-1). This CXCR4-mediated HIV-1 fusion can be inhibited by SDF-1. Because of its importance in the study of immunity and AIDS, large scale production of SDF-1 is desirable. In addition to recombinant technology, chemical synthesis provides means by which biologically active proteins can be produced not only in large quantity but also with a variety of designed modifications. In this study, we investigated the binding and function of an SDF-1alpha analogue, N33A, synthesized by a newly developed native chemical ligation approach. Radioiodinated N33A showed high affinity binding to human monocytes, T lymphocytes, as well as neutrophils, and competed equally well with native recombinant SDF-1alpha for binding sites on leukocytes. N33A also showed equally potent chemoattractant activity as native recombinant SDF-1alpha for human leukocytes. Further study with CXCR4/Fusin/LESTR transfected HEK 293 cells showed that N33A binds and induces directional migration of these cells in vitro. These results demonstrate that the chemically synthesized SDF-1alpha analogue, N33A, which can be produced rapidly in large quantity, possesses the same capacity as native SDF-1alpha to activate CXCR4-expressing cells and will provide a valuable agent for research on the host immune response and AIDS.
Laterally Extended Naphthalene Tetracarboxylic Bisimides (2010)

The colorless naphthalene tetracarboxylic bisimides were laterally extended with imidazole rings to give colored and highly fluorescent materials. Donor-substituted phenyl groups at the imidazole rings cause further bathochromic shifts in fluorescence where the strong solvent influence in the spectra is typical for a TICT process. Applications are discussed.
Nonisotopic competitive RT-PCR assay to measure MDR1 gene expression (1995)

We report an original application of competitive reverse transcription-polymerase chain reaction (RT-PCR) for the quantification of MDR1 mRNA in clinical specimens by simultaneous reverse transcription and PCR amplification of cellular RNA with decreasing amounts of an internal standard. The competitor RNA shares the same MDR1 primer sequences as the cellular mRNA, but yields a different-sized PCR product. This allows resolution of the amplified cDNA fragments after agarose gel electrophoresis and ethidium bromide staining. The concentration of MDR1 mRNA is derived from the ratio between the intensities of the bands corresponding to the amplified products. We have used this assay to measure MDR1 expression in breast carcinomas and assessed the precision, sensitivity, and accuracy of the method. Competitive RT-PCR is a simple, highly specific, nonradioactive procedure for the quantification of MDR1 mRNA and is particularly suitable for use in the clinical laboratory.
Introduction (2002)

Co-existing with China will be one of the great challenges facing the West in the twenty-first century. Indeed some see it as likely to be the greatest challenge, with China having the potential to develop as a super power capable of matching the West, in particular the United States, economically, politically, militarily. Whether or not that happens, there seems no doubt that, given the collapse of the Soviet empire, China is likely to emerge as the main counterweight to any continuing Western dominance in world affairs.
Paper E – Questions (2012)

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EUDRAGIT POLYMERS IN COLON TARGETED ORAL DELIVERY OF INSULIN (2014)

The colon targeted drug delivery has a number of important implications in the field of pharmacotherapy. Oral colon targeted drug delivery of insulin has recently gained attention. Targeting of insulin to the colon via oral administration protect the insulin from degradation or release in the stomach and small intestine. It also ensures abrupt or controlled release of the insulin in the proximal colon. Various drug delivery systems have been designed that deliver the drug quantitatively to the colon and then trigger the release of drug. This review will cover different types of Eudragit polymers which can be used in formulation of colon targeted drug delivery systems.
Activation Energies for Dissociation of Double Strand Oligonucleotide Anions:  Evidence for Watson−Crick Base Pairing in Vacuo (1998)

The dissociation kinetics of a series of complementary and noncomplementary DNA duplexes, (TGCA)(2) (3-), (CCGG)(2) (3-), (AATTAAT)(2) (3-), (CCGGCCG)(2) (3-), A(7)*T(7) (3-), A(7)*A(7) (3-), T(7)*T(7) (3-), and A(7)*C(7) (3-) were investigated using blackbody infrared radiative dissociation in a Fourier transform mass spectrometer. From the temperature dependence of the unimolecular dissociation rate constants, Arrhenius activation parameters in the zero-pressure limit are obtained. Activation energies range from 1.2 to 1.7 eV, and preexponential factors range from 10(13) to 10(19) s(-1). Dissociation of the duplexes results in cleavage of the noncovalent bonds and/or cleavage of covalent bonds leading to loss of a neutral nucleobase followed by backbone cleavage producing sequence-specific (a - base) and w ions. Four pieces of evidence are presented which indicate that Watson-Crick (WC) base pairing is preserved in complementary DNA duplexes in the gas phase: i. the activation energy for dissociation of the complementary dimer, A(7)*T(7) (3-), to the single strands is significantly higher than that for the related noncomplementary A(7)*A(7) (3-) and T(7)*T(7) (3-) dimers, indicating a stronger interaction between strands with a specific base sequence, ii. extensive loss of neutral adenine occurs for A(7)*A(7) (3-) and A(7)*C(7) (3-) but not for A(7)*T(7) (3-) consistent with this process being shut down by WC hydrogen bonding, iii. a correlation is observed between the measured activation energy for dissociation to single strands and the dimerization enthalpy (-DeltaH(d)) in solution, and iv. molecular dynamics carried out at 300 and 400 K indicate that WC base pairing is preserved for A(7)*T(7) (3-) duplex, although the helical structure is essentially lost. In combination, these results provide strong evidence that WC base pairing can exist in the complete absence of solvent.