gebhart/patrepeval huggingface dataset:
[Comparative study of the effectiveness of several anti-arrhythmia preparations in patients with a frequent extrasystole]. (1986)

The efficiency and side effect rates of 7 antiarrhythmic drugs: mexitil, rhytmilen, propranolol, ethmosine, cordarone, finoptin, digoxin, were compared in 80 patients. Their heart rhythm was controlled by continuous Holter ECG monitoring. The efficacy of oral doses of the drugs was assessed both in acute pharmacologic tests and in short-term clinical courses. The smallest side-effect rates and the highest efficiency were shown by cordarone (in all clinical samples) and ethmosine (particularly in coronary patients), hence both can be recommended for long-term clinical use.
An ethnographic object-oriented analysis of explorer presence in a volcanic terrain environment: Claims and evidence (1994)

An ethnographic field study was conducted to investigate the nature of presence in field geology, and to develop specifications for domain-based planetary exploration systems utilizing virtual presence. Two planetary geologists were accompanied on a multi-day geologic field trip that they had arranged for their own scientific purposes, which centered on an investigation of the extraordinary xenolith/nodule deposits in the Kaupulehu lava flow of Hualalai Volcano, on the island of Hawaii. The geologists were observed during the course of their field investigations and interviewed regarding their activities and ideas. Analysis of the interview resulted in the identification of key domain entities and their attributes, relations among the entities, and explorer interactions with the environment. The results support and extend the author's previously reported continuity theory of presence, indicating that presence in field geology is characterized by persistent engagement with objects associated by metonymic relations. The results also provide design specifications for virtual planetary exploration systems, including an integrating structure for disparate data integration. Finally, the results suggest that unobtrusive participant observation coupled with field interviews is an effective research methodology for engineering ethnography.
Self-control in casual games: The relationship between Candy Crush Saga&#x2122; players' in-app purchases and self-control (2014)

Casual Games and free-to-play games have recently rapidly Increased In popularity, perhaps In part because of the success of in-app purchases and micro-transactions as an economic model. While these games are often touted for their success In the gaming market, the effect on players when faced with such frequent purchasing decisions in-game is not well-studied. Theories of self-control suggest that people have limited resource pools of self-control, and facing frequent frustration and purchasing decisions may deplete this resource. In this paper, we present the results of a Mechanical Turk study on a popular casual game, Candy Crush Saga™, to investigate various factors impacting player behaviour, with a specific focus on self-control. Our study reveals that the amount players spend on in-app purchases is correlated with lower levels of self-control. On the other hand, purchases and self-control levels were not significantly correlated with the amount of time people play, game addiction, or problem video game playing. We present design recommendations which can be applied to existing or new game designs in terms of both the economics of games and the psychology of games, including mechanics to account for low self-control and to avoid negative effects on self-control.
Share of extracellular mitochondrial DNA with a mutations increases in the plasma of patients lung cancer after radiotherapy (2010)

Quantitative and qualitative changes in circulating extracellular DNA (ec-DNA) of blood plasma are considered as markers for diagnosis and prognostic of tumor pathology. We investigated the content of mutant copies of the circulating extracellular mitochondrial DNA (ec-mtDNA) in blood plasma (using the enzymatic method, based on the cleavage of DNA with unpaired bases by CEL-I endonuclease) in 8 patients with lung cancer before and after radiotherapy, as well as in healthy young and elderly donors. It was found that in the plasma of healthy elderly donors share of ec-mtDNA with mutations (consisting of total circulating DNA) is much greater, than that of young donors. On the other hand, in the plasma of lung cancer patients (aged 70-76 years) before radiotherapy a substantial increase in the share of ec-mtDNA with mutations, compared with that of healthy elderly donors. Following radiotherapy, patients with lung cancer found a twofold increase of the proportion of ec-mtDNA with mutations in the total circulating plasma DNA. This increase is largely, perhaps due to the release of ec-mtDNA with mutations from dying tumor cells and cells damaged by normal tissues.
Using 45S5 bioglass cones as endosseous ridge maintenance implants to prevent alveolar ridge resorption: a 5-year evaluation. (1997)

Bioglass cones acting as space fillers after removal of tooth roots delay the resorption of alveolar ridges. In 1987, 242 implants in 29 patients with a mean postimplantation interval of 19.9 months were reported by the authors. Bioglass cones had been fitted snugly at least 2 mm below the alveolar crest, and dentures were placed no sooner than 6 weeks following tooth removal; 2.9% had been lost and 3.7% developed dehiscences. The present report on 168 implants in 20 recalled patients (mean postimplantation interval of 63.2 months) revealed a loss of 14.3% of the implants and 7.7% of the implants requiring recontouring. Literature indicates highest survival rates for implants in the anterior mandible; however, the present data demonstrate a statistically significant retention rate in the anterior maxilla. With this high rate of Bioglass cone retention (85.7%) after 5 years, their placement into fresh sockets to maintain the alveolar ridge is recommended.
Enteroaggregative<i>Escherichia coli</i>Produce Intestinal Inflammation and Growth Impairment and Cause Interleukin‐8 Release from Intestinal Epithelial Cells (1998)

Enteroaggregative E. coli (EAggEC) are emerging as an important cause of persistent diarrhea, especially in children in the developing world, yet the pathogenesis of EAggEC infection is poorly understood. In an ongoing prospective study of childhood diarrhea in an urban Brazilian slum, EAggEC are the leading cause of persistent diarrhea. Children from this study with EAggEC and persistent diarrhea had significant elevations in fecal lactoferrin, interleukin (IL)-8, and IL-1beta. Moreover, children with EAggEC without diarrhea had elevated fecal lactoferrin and IL-1beta concentrations. The children with EAggEC in their stool had significant growth impairment after their positive culture, regardless of the presence or absence of diarrhea. Finally, 2 EAggEC strains were shown to cause IL-8 release from Caco-2 cells, apparently via a novel heat-stable, high-molecular-weight protein. These findings suggest that EAggEC may contribute to childhood malnutrition, trigger intestinal inflammation in vivo, and induce IL-8 secretion in vitro.
Improved 2D nano-LC/MS for proteomics applications: a comparative analysis using yeast proteome. (2004)

The most commonly used method for protein identification with two-dimensional (2D) online liquid chromatography-mass spectrometry (LC/MS) involves the elution of digest peptides from a strong cation exchange column by an injected salt step gradient of increasing salt concentration followed by reversed phase separation. However, in this approach ion exchange chromatography does not perform to its fullest extent, primarily because the injected volume of salt solution is not optimized to the SCX column. To improve the performance of strong cation exchange chromatography, we developed a new method for 2D online nano-LC/MS that replaces the injected salt step gradient with an optimized semicontinuous pumped salt gradient. The viability of this method is demonstrated in the results of a comparative analysis of a complex tryptic digest of the yeast proteome using the injected salt solution method and the semicontinuous pump salt method. The semicontinuous pump salt method compares favorably with the commonly used injection method and also with an offline 2D-LC method.
In-depth Analyses of Kinase-dependent Tyrosine Phosphoproteomes Based on Metal Ion-functionalized Soluble Nanopolymers (2010)

The ability to obtain in-depth understanding of signaling networks in cells is a key objective of systems biology research. Such ability depends largely on unbiased and reproducible analysis of phosphoproteomes. We present here a novel proteomics tool, polymer-based metal ion affinity capture (PolyMAC), for the highly efficient isolation of phosphopeptides to facilitate comprehensive phosphoproteome analyses. This approach uses polyamidoamine dendrimers multifunctionalized with titanium ions and aldehyde groups to allow the chelation and subsequent isolation of phosphopeptides in a homogeneous environment. Compared with current strategies based on solid phase micro- and nanoparticles, PolyMAC demonstrated outstanding reproducibility, exceptional selectivity, fast chelation times, and high phosphopeptide recovery from complex mixtures. Using the PolyMAC method combined with antibody enrichment, we identified 794 unique sites of tyrosine phosphorylation in malignant breast cancer cells, 514 of which are dependent on the expression of Syk, a protein-tyrosine kinase with unusual properties of a tumor suppressor. The superior sensitivity of PolyMAC allowed us to identify novel components in a variety of major signaling networks, including cell migration and apoptosis. PolyMAC offers a powerful and widely applicable tool for phosphoproteomics and molecular signaling.
SEU Mitigation Techniques for Virtex FPGAs in Space Applications (1999)

SRAM based logic devices such as FPGAs have some susceptibility to SEU and functional interruption. This paper describes several reliable mitigation techniques for the Virtex series FPGA architecture, which will retain functional integrity while static upsets are detected and corrected. Additionally, this paper demonstrates how an SEU in an FPGA can be corrected in 3us without disrupting operation of the device, how to build hardened voting circuits, and that a single event has only 1 chance out of 3.25 million of causing a functional interrupt.
Cross-layer design proposals for wireless mobile networks: A survey and taxonomy (2008)

Third-generation (3G) and beyond 3G mobile communication systems must provide interoperability with the Internet, increase throughput for mobile devices, and optimize their operation for multimedia applications. The limited ability of traditional layered architectures to exploit the unique nature of wireless communication has fostered the introduction of cross-layer design solutions that allow optimized operation for mobile devices in the modern heterogeneous wireless environment. In this article we present the major cross-layer design solutions that handle such problems, and discuss cross-layer implementations with a focus on functional entities that support cross-layer processes and the respective signaling. In addition, we consider the associated architectural complexity and communication overhead they introduce. Furthermore, we point out the major open technical challenges in the cross-layer design research area. Finally, we conclude our article with a summary of cross-layer approaches developed thus far and provide directions for future work.
VPAC Receptor Modulation of Neuroexcitability in Intracardiac Neurons (2004)

Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) have been found within mammalian intracardiac ganglia, but the cellular effects of these neuropeptides remain poorly understood. Fluorometric calcium imaging and whole cell patch clamp recordings were used to examine the effects of PACAP and VIP on [Ca2+]i and neuroexcitability, respectively, in intracardiac neurons of neonatal rats. PACAP and VIP evoked rapid increases in [Ca2+]i that exhibited both transient and sustained components. Pharmacological experiments using PAC1 and VPAC receptor-selective antagonists demonstrated that the elevations in [Ca2+]i result from the activation of VPAC receptors. The transient increases in [Ca2+]i were shown to be the product of Ca2+ mobilization from caffeine/ryanodine-sensitive intracellular stores and were not due to inositol 1,4,5-trisphosphate-mediated calcium release. In contrast, the sustained [Ca2+]i elevations were dependent on extracellular Ca2+ and were blocked by the transient receptor channel antagonist, 2-aminoethoxydiphenyl borate, which suggests that they are due to Ca2+ entry via store-operated channels. In addition to elevating [Ca2+]i, both PACAP and VIP depolarized intracardiac neurons, and PACAP was further shown to augment action potential firing in these cells. Depolarization of intracardiac neurons by the neuropeptides was dependent on activation of VPAC receptors and the concomitant increases in [Ca2+]i. Although activation of PAC1 receptors alone had no direct effects on neuroexcitability, PAC1 receptor stimulation potentiated the VPAC receptor-induced depolarizations. Furthermore, enhanced action potential firing was only observed upon concurrent stimulation of PAC1 and VPAC receptors, which indicates that these receptors act synergistically to enhance neuroexcitability in intracardiac neurons.
Virtual Sensors of Tire Pressure and Road Friction (2001)

<div class="htmlview paragraph">The idea of a virtual sensor is to extract information of parameters that cannot be measured directly, or at least would require very costly sensors, by only using available information. Virtual sensors are described for the friction between road and tire, the tire inflation pressure and wheel imbalance. There are certain interconnections between these virtual sensors so they are preferably implemented in one unit. Results from a real-time implementation, using mainly sensor information from the CAN bus, are given.</div>
Multireflection Planar Time-of-Flight Mass Analyzer. II: The High-Resolution Mode (2005)

The feasibility of the high-resolution operating mode in a planar multireflection time-of-flight analyzer that is suggested in Part I of this work is demonstrated. Time-of-flight aberrations, which limit the resolution, are estimated. A resolution as high as 200 000 at a time of flight of 70 ms is achieved in experiments. It is shown that the maximal resolution is limited by the duration of the ion packet generated by the source. The resolution can be improved by closing the ion beam trajectory with the formation of repeating cycles. The number of the cycles depends on the beam intensity losses due to scattering by the residual gas. It seem likely that the resolution can be improved further by using a higher vacuum, refining the ion source, and applying more stable power supplies.
Potential Role for Hyaluronan and the Hyaluronan Receptor RHAMM in Mobilization and Trafficking of Hematopoietic Progenitor Cells (1999)

Although the mechanism(s) underlying mobilization of hematopoietic progenitor cells (HPCs) is unknown, detachment from the bone marrow (BM) microenvironment and motility are likely to play a role. This work analyzes the motile behavior of HPCs and the receptors involved. CD34(+)45(lo/med)Scatterlo/med HPCs from granulocyte colony-stimulating factor (G-CSF)-mobilized blood and mobilized BM were compared with steady-state BM for their ability to bind hyaluronan (HA), their expression of the HA receptors RHAMM and CD44, and their motogenic behavior. Although RHAMM and CD44 are expressed by mobilized blood HPCs, function blocking monoclonal antibodies (MoAbs) identified RHAMM as a major HA binding receptor, with a less consistent participation by CD44. Permeabilization of mobilized blood HPCs showed a pool of intracellular (ic) RHAMM and a smaller pool of icCD44. In contrast, steady-state BM HPCs have significantly larger pools of icRHAMM and icCD44. Also, in contrast to mobilized blood HPCs, for steady-state BM HPCs, MoAbs to RHAMM and CD44 act as agonists to upregulate HA binding. The comparison between mobilized and steady-state BM HPCs suggests that G-CSF mobilization is associated with depletion of intracellular stores of HA receptors and modulates HA receptor usage. To confirm that mobilization alters the HA receptor distribution and usage by HPCs, samples of BM were collected at the peak of G-CSF mobilization in parallel with mobilized blood samples. HA receptor distribution of mobilized BM HPCs was closely matched with mobilized blood HPCs and different from steady-state BM HPCs. Mobilized BM HPCs had lower pools of icHA receptors, similar to those of mobilized blood HPCs. Treatment of mobilized BM HPCs with anti-RHAMM MoAb decreased HA binding, in contrast to steady-state BM HPCs. Thus, G-CSF mobilization may stimulate an autocrine stimulatory loop for HPCs in which HA interacts with basal levels of RHAMM and/or CD44 to stimulate receptor recycling. Consistent with this, treatment of HPCs with azide, nystatin, or cytochalasin B increased HA binding, implicating an energy-dependent process involving lipid rafts and the cytoskeleton. Of the sorted HPCs, 66% were adherent and 27% were motile on fibronectin plus HA. HPC adherence was inhibited by MoAbs to beta1 integrin and CD44, but not to RHAMM, whereas HPC motility was inhibited by MoAb to RHAMM and beta1 integrin, but not to CD44. This finding suggests that RHAMM and CD44 play reciprocal roles in adhesion and motility by HPCs. The G-CSF-associated alterations in RHAMM distribution and the RHAMM-dependent motility of HPCs suggest a potential role for HA and RHAMM in trafficking of HPCs and the possible use of HA as a mobilizing agent in vivo.
Statistical limitations in x‐ray reconstructive tomography (1976)

Statistical fluctuations, or noise, in reconstructed tomograms are analyzed as a function of x‐ray energy. The result is essentially constant for energies above 50 keV, assuming constant dose and spatial resolution, and is in close agreement with the actual performance of the EMI‐Scanner (water‐bag model). The noise is inversely proportional to the square of cell dimensions. For scanners without a water bag, the theoretical noise is approximately 30% less.
Carbon Nanotube-Templated Assembly of Protein (2006)

This paper describes a novel general strategy for fabricating protein-polyion multilayers by electrostatic layer-by-layer (LBL) self-assembly on carbon nanotube templates. Such a noncovalent functionalization method is important for preserving the activity of biomolecular, the mechanical, and electrical properties of carbon nanotubes. Glucose oxidase and poly(diallyldimethylammonium) chloride polymer (PDDA) were used as models to investigate the LBL process on CNT templates. High-resolution TEM and electrochemical characterization confirm the formation of LBL nanostructures on carboxyl functionalized carbon nanotubes. We have also demonstrated the applications of these nanoshell bioreactors to direct electrochemistry of protein and biosensing. This strategy can be applied to assemble other biological molecules such as antibody, antigen, and DNA for wide bioassay applications.
Adeno-Associated Viral Gene Delivery in Neurodegenerative Disease (2011)

The advent of viral gene therapy technology has contributed greatly to the study of a variety of medical conditions, and there is increasing promise for clinical translation of gene therapy into human treatments. Adeno-associated viral (AAV) vectors provide one of the more promising approaches to gene delivery, and have been used extensively over the last 20 years. Derived from nonpathogenic parvoviruses, these vectors allow for stable and robust expression of desired transgenes in vitro and in vivo. AAV vectors efficiently and stably transduce neurons, with some strains targeting neurons exclusively in the brain. Thus, AAV vectors are particularly useful for neurodegenerative diseases, which have led to numerous preclinical studies and several human trials of gene therapy in patients with Parkinson's disease, Alzheimer's disease, and pediatric neurogenetic disorders. Here, we describe an efficient and reliable method for the production and purification of AAV serotype 2 vectors for both in vitro and in vivo applications.
Binary cooperative complementary nanoscale interfacial materials (2000)

Abstract Binary cooperative complementary nanoscale interfacial materials, i.e, materials with two complementary properties on the nanoscale, are introduced as a new concept for the design of functional materials. The concept is based on the generation of nanostructures with mutually compensating properties on the surface of a solid. Under certain coordinating conditions, unexpected properties may often appear at these kinds of interfaces, creating a huge potential for applications and theoretical research. Recent research indicates that the binary cooperative complementary concept is extremely useful for the design and creation of nanoscale functional materials.
[Clinical observation of tramadol on postoperative painment from LASEK]. (2005)

tramadol plays an important role in efficacy and safety for controlling ocular pain and inflammation after LASEK.
ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in <i>E. coli</i> (2012)

Harnessing cell factories for producing biofuel and pharmaceutical molecules has stimulated efforts to develop novel synthetic biology tools customized for modular pathway engineering and optimization. Here we report the development of a set of vectors compatible with BioBrick standards and its application in metabolic engineering. The engineered ePathBrick vectors comprise four compatible restriction enzyme sites allocated on strategic positions so that different regulatory control signals can be reused and manipulation of expression cassette can be streamlined. Specifically, these vectors allow for fine-tuning gene expression by integrating multiple transcriptional activation or repression signals into the operator region. At the same time, ePathBrick vectors support the modular assembly of pathway components and combinatorial generation of pathway diversities with three distinct configurations. We also demonstrated the functionality of a seven-gene pathway (~9 Kb) assembled on one single ePathBrick vector. The ePathBrick vectors presented here provide a versatile platform for rapid design and optimization of metabolic pathways in E. coli.
Acute and subacute bronchial effects of oral cannabinoids (1984)

The bronchodilating activity of oral cannabinoids was evaluated in three double-blind experiments that involved the study of dose-response and interactive relationships and the potential development of tolerance. Data indicated that delta 8-tetrahydrocannabinol (delta 8-THC), cannabinol (CBN), and cannabidiol (CBD) in maximal doses of 75 mg, 1200 mg, and 1200 mg, respectively, did not induce significant dose-related physiologic effects in experienced marijuana smokers. delta 8-THC (75 mg) was, however, associated with bronchodilation, tachycardia, and peak highs less than that after delta 9-tetrahydrocannabinol (delta 9-THC). The combinations of CBN and CBD with low-dose delta 9-THC (5 mg) did not induce significant bronchodilation but did exert interactive effects on heart rate and "high." A 20-day study of daily delta 9-THC (20 mg), CBN (600 mg), and CBD (1200 mg) did not indicate tolerance or reverse tolerance to any drug. We conclude that delta 9-THC and, to a lesser extent, delta 8-THC, have acute bronchodilator activity but that CBN, CBD, and their combinations do not provide effective bronchodilation. The daily use of delta 9-THC was not associated with clinical tolerance.
Highly efficient parametric conversion of femtosecond Ti:sapphire laser pulses at 1 kHz (1994)

Pulses with energies as high as 150 microJ and durations as low as 60 f(s) were generated from 1.1 to 2.6 microm by a traveling-wave parametric converter pumped by femtosecond pulses of a Ti:sapphire laser with chirped-pulse amplification.