gebhart/patrepeval huggingface dataset:
Emergence of insulin receptors upon alloimmune T cells in the rat. (1977)

Insulin, as well as other ligands which increase intracellular guanosine 3',5'-cyclic monophosphate (cGMP), augments thymic-derived (T)- lymphocyte effector activity as revealed by alloimmune lymphocyte-mediated cytotoxicity. The observation that insulin binds only to monocytes among circulating nonimmune human mononuclear cells fosterd reexamination of the mechanism by which insulin augments T-lymphocyte function. This report concerns a test of the hypothesis that the T cell is directly affected by insulin and that an insulin receptor emerges upon T lymphocytes consequent to immune activation. Spleens were removed from rats skin grafted across a major histocompatibility barrier. Lymphocytes were harvested from Ficoll-Hypaque density gradients and subsequently enriched for T cells by passage over one or two nylon wool columns. This population was composed of more than 98% T cells as assessed by surface marker techniques (Ig staining, erythrocyte antibody, and erythrocyte antibody complement rosetting, anti-T staining). There was no loss of augmentation of lymphocyte-mediated cytotoxicity induced by insulin, carbamycholine, and 8-bromo-cGMP in the purified cells when compared to unfractionated cells 7 days after transplantation. 125I-insulin bound saturably to the allostimulated T-enriched lymphocytes with maximum binding at 12.8 +/- 0.2 pg and a dissociation constant at equilibrium of 1.3 nM. In contrast, insulin receptors were not present on nonimmune T-enriched cells or on T cells from animals that received syngeneic grafts. The affinity of the lymphocyte insulin receptor was similar to that of more conventional insulin-sensitive tissues e.g., liver, adipocyte. After 89% of T cells from spleens on day 7 were lysed with anti-thy 1.1 antibody and complement, the ability to measure specific insulin binding was lost. These data confirm a physiologic role for insulin in T-lymphocyte effector function and describe the emergence of insulin receptors concomitant with cell sensitivity to ligand. Such receptors may play a role in hormonal modulation of the immune response.
Green Biosynthesis Silver Nanoparticles Using Leaf Extract (2018)

Using plant extract to synthesize silver nanoparticles is particularly worth of attention due to the simple, quick, affordable and friendly protocol with the environment. In our experiment, we have proved both the ability of common species (Mentha aquatic, Coleus blumei and Tagetes erecta) of achieving the synthesis of nanoparticles with a diameter between 8 and 300 nm, and the anti-bacterian effects of the obtained nano-silver.
An analysis of C machine support for other block-structured languages (1983)

Although the C machine proposed by Ditzel ( et al .) was originally intended to execute the C programming language, its utility would be enhanced if it could also execute programs written in other languages. Of particular interest is the Ada programming language, in part because it is likely to be a standard for government work, but more importantly because its support requirements are typical of many modern languages such as CHILL, MESA, EUCLID, MODULA, and others. The most salient characteristics of the C machine, e.g. its stack cache and address modes, are seen to map well onto the Ada language. In fact, many of the arguments for these features are more compelling for the Ada language than they are for C. One exception is the way the stack cache may perform on a heavily multi-tasked program, but there is no obvious solution to this, even on much more complex machines like the VAX11-780.
Comparative Study on Risk Perception and Coping Behavior of Beijing Residents During SARS Period (2004)

Objective:To investigate the risk perception and coping behavior of Beijing residents during SARS period.Methods:We investigated,at random,895 residents during May 5th～11th and 582 residents during May 27th-June 7th with questionnaires.Results:Residents tended to make their judgment on SARS after integrating the information from different sources when SARS was in a platform period; they paid more attention to the controllability of SARS when SARS cases increased dramatically and to the infection of SARS when it was in a platform period;the focus on negative information can increase residents' risk perception to SARS, which can predict the positive and negative presentiment indexes when SARS increased dramatically, while the negative information would decrease residents' risk perception, which still can predict the negative presentiment indexes but can't predict the positive ones when SARS was in a platform period.Conclusion:The residents deal with the information about SARS in a more rational manner when SARS was in a platform period,which influenced their risk perception to SARS and the presentiment indexes.
Effect of age on<i>in vivo</i>rates of mitochondrial protein synthesis in human skeletal muscle (1996)

A progressive decline in muscle performance in the rapidly expanding aging population is causing a dramatic increase in disability and health care costs. A decrease in muscle endurance capacity due to mitochondrial decay likely contributes to this decline in muscle performance. We developed a novel stable isotope technique to measure in vivo rates of mitochondrial protein synthesis in human skeletal muscle using needle biopsy samples and applied this technique to elucidate a potential mechanism for the age-related decline in the mitochondrial content and function of skeletal muscle. The fractional rate of muscle mitochondrial protein synthesis in young humans (24 +/- 1 year) was 0.081 +/- 0.004%.h-1, and this rate declined to 0.047 +/- 0.005%.h-1 by middle age (54 +/- 1 year; P < 0.01). No further decline in the rate of mitochondrial protein synthesis (0.051 +/- 0.004%.h-1) occurred with advancing age (73 +/- 2 years). The mitochondrial synthesis rate was about 95% higher than that of mixed protein in the young, whereas it was approximately 35% higher in the middle-aged and elderly subjects. In addition, decreasing activities of mitochondrial enzymes were observed in muscle homogenates (cytochrome c oxidase and citrate synthase) and in isolated mitochondria (citrate synthase) with increasing age, indicating declines in muscle oxidative capacity and mitochondrial function, respectively. The decrease in the rates of mitochondrial protein synthesis is likely to be responsible for this decline in muscle oxidative capacity and mitochondrial function. These changes in muscle mitochondrial protein metabolism may contribute to the age-related decline in aerobic capacity and muscle performance.
Human Papillomavirus Detection in Head and Neck Squamous Cell Carcinomas (2010)

Squamous cell carcinomas of the head and neck (HNSCC) are a frequent diagnosis in anatomic pathology practice. Tobacco use and heavy alcohol consumption are known risk factors for HNSCC but in other cases human papillomavirus (HPV) is linked to carcinogenesis. HPV proteins E6 and E7 promote oncogenesis by blocking the action of p53 and pRB, respectively. An absence of p53 mutations in addition to expression of p16 are part of the distinct molecular profile identified in the subset of HNSCCs because of HPV. Various methods are available for HPV detection but polymerase chain reaction and in situ hybridization techniques are commonly used. Both methods are amenable for testing formalin-fixed paraffin-embedded tissue that is a sample type readily available to the pathologist. HPV is detectable in approximately a quarter of all HNSCCs, and is particularly prevalent in the oropharynx in which the positivity rates approach 40%. A vast majority of HPV-related HNSCCs are owing to HPV type 16 with types 18, 31, and 33 accounting for almost all of the remaining cases. HPV-related HNSCCs are associated with better prognosis for both recurrence and survival. This group of tumors has also been shown to respond well to radiation treatment. As the clinical relevance of HPV in HNSCCs continues to emerge, anatomic pathologists are likely to receive increasing requests for testing. Herein, the authors review the biological and clinical aspects of HPV-associated HNSCC and review techniques for HPV detection.
Sustained delivery of retinoic acid from microspheres of biodegradable polymer in PVR. (1993)

The authors obtained a sustained release of RA from PLGA microspheres in vitro for 40 days. A single injection of RA-loaded microspheres in suspension in BSS was effective in reducing the incidence of TRD after 2 months in a rabbit model of PVR.
Optimization of Orally Bioavailable Enhancer of Zeste Homolog 2 (EZH2) Inhibitors Using Ligand and Property-Based Design Strategies: Identification of Development Candidate (<i>R</i>)-5,8-Dichloro-7-(methoxy(oxetan-3-yl)methyl)-2-((4-methoxy-6-methyl-2-oxo-1,2-dihydropyridin-3-yl)methyl)-3,4-dihydroisoquinolin-1(2<i>H</i>)-one (PF-06821497) (2017)

A new series of lactam-derived EZH2 inhibitors was designed via ligand-based and physicochemical-property-based strategies to address metabolic stability and thermodynamic solubility issues associated with previous lead compound 1. The new inhibitors incorporated an sp<sup>3</sup> hybridized carbon atom at the 7-position of the lactam moiety present in lead compound 1 as a replacement for a dimethylisoxazole group. This transformation enabled optimization of the physicochemical properties and potency compared to compound 1. Analysis of relationships between calculated log D (clogD) values and in vitro metabolic stability and permeability parameters identified a clogD range that afforded an increased probability of achieving favorable ADME data in a single molecule. Compound 23a exhibited the best overlap of potency and pharmaceutical properties as well as robust tumor growth inhibition in vivo and was therefore advanced as a development candidate (PF-06821497). A crystal structure of 23a in complex with the three-protein PRC2 complex enabled understanding of the key structural features required for optimal binding.
An integrating framework for efficient NFV monitoring (2016)

Most challenges associated with Network Functions Virtualisation (NFV) are related to the automated and large-scale deployment of virtualised network functions (VNFs) within an operational infrastructure, as well as their availability and performance. In order to effectively face these challenges, efficient and comprehensive monitoring of NFV services is a critical aspect. This paper discusses the challenges of proper NFV monitoring, focusing on the process of collecting NFV Infrastructure (NFVI) metrics and processing them at Virtualised Infrastructure Management (VIM) level. It also presents an open-source integrating framework for efficient NFV monitoring, developed in the frame of the EU-funded project T-NOVA. The presented framework enables agent-based and agent-less infrastructure monitoring as well as VNF application metrics collection, aggregation, processing and exposure in a lightweight and scalable manner.
Insect Attractants, The Development of Male Melon Fly Attractants (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInsect Attractants, The Development of Male Melon Fly AttractantsB. H. Alexander, Morton Beroza, T. A. Oda, L. F. Steiner, D. H. Miyashita, and W. C. MitchellCite this: J. Agric. Food Chem. 1962, 10, 4, 270–276Publication Date (Print):July 1, 1962Publication History Published online1 May 2002Published inissue 1 July 1962https://pubs.acs.org/doi/10.1021/jf60122a001https://doi.org/10.1021/jf60122a001research-articleACS PublicationsRequest reuse permissionsArticle Views161Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A widely expressed novel C2H2 zinc-finger protein with multiple consensus phosphorylation sites is conserved in mouse and man (1995)

We have cloned a murine cDNA whose deduced sequence encodes a 455-amino-acid (aa) zinc-finger protein (Zfp), PZf (penta Zf protein). Sequence analysis shows that PZf has multiple phosphorylation consensus sites for casein kinase II and protein kinase C in its N-terminal portion. This region (aa 1 to 197), which does not share significant homology to known aa sequences, has a number of charged (39 Glu, 15 Asp, 23 Lys, 22 Arg) and hydroxyl (20 Ser, 12 Thr) aa. This potentially phosphorylatable region is followed by five C2H2 Zf in the middle of the protein. The Pzf gene is transcribed as a 5-kb mRNA in all murine tissues examined. The human genome also seems to contain one or more Pzf-related genes.
Power-Combining Transformer Techniques for Fully-Integrated CMOS Power Amplifiers (2008)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> Fully integrated CMOS power amplifiers (PAs) with parallel power-combining transformer are presented. For the high power CMOS PA design, two types of transformers, series-combining and parallel-combining, are fully analyzed and compared in detail to show the parasitic resistance and the turn ratio as the limiting factor of power combining. Based on the analysis, two kinds of parallel-combining transformers, a two-primary with a 1:2 turn ratio and a three-primary with a 1:2 turn ratio, are incorporated into the design of fully-integrated CMOS PAs in a standard 0.18-<formula formulatype="inline"> <tex>$\mu \hbox{m}$</tex></formula> CMOS process. The PA with a two-primary transformer delivers 31.2 dBm of output power with 41% of power-added efficiency (PAE), and the PA with a three-primary transformer achieves 32 dBm of output power with 30% of PAE at 1.8 GHz with a 3.3-V power supply. </para>
Synthesis of Carbon Encapsulated Magnetic Nanoparticles with Giant Coercivity by a Spray Pyrolysis Approach (2007)

Carbon encapsulated magnetic (metal) nanoparticles (CEMNPs) have wide applications in biomedicine and the magnetic recording industry. However, synthesis of such particles with a high coercive force and good ferromagnetism is still a great challenge. The present study reports a new method for the continuous production of CEMNPs of high purity. This involves the spray pyrolysis of a mixture of iron pentacarbonyl and ethanol at 500-900 degrees C. Results show that the Fe (or Fe3C) particles synthesized at 700 and 900 degrees C were well encapsulated by graphitic layers with rare byproducts such as carbon nanotubes, nanofibers, or bulk amorphous carbon. Those synthesized at 700 degrees C had a particle size of 30-50 nm, a giant coercive force of 867 Oe, and a good magnetic remanence of 33% at room temperature. The present approach based on spray pyrolysis is advantageous over previous ones in suitability for large-scale production, and the synthesized material has wide applications in many fields.
Vascular Endothelial Growth Factor Receptor-1 and Neuropilin-2 Form Complexes (2001)

The products of the neuropilin-1 (Np-1) and neuropilin-2 (Np-2) genes are receptors for factors belonging to the class 3 semaphorin family and participate in the guidance of growing axons to their targets. In the presence of heparin-like molecules, both receptors also function as receptors for the heparin-binding 165-amino acid isoform of vascular endothelial growth factor (VEGF(165)). Both receptors are unable to bind to the 121-amino acid isoform of vascular endothelial growth factor (VEGF(121)), which lacks a heparin-binding domain. Interestingly, complexes corresponding in size to (125)I-VEGF(121).neuropilin complexes are formed when (125)I-VEGF(121) is bound and cross-linked to porcine aortic endothelial cells co-expressing VEGFR-1 and either Np-1 or Np-2. These complexes do not seem to represent complexes of (125)I-VEGF(121) with a truncated form of VEGFR-1, presumably formed as a result of the presence of Np-1 or Np-2 in the cells, because such truncated forms could not be detected with anti-VEGFR-1 antibodies. Antibodies directed against VEGFR-1 co-immunoprecipitated the (125)I-VEGF(121).Np-2 sized cross-linked complex along with (125)I-VEGF(121).VEGFR-1 complexes from cells expressing both VEGFR-1 and Np-2 but not from control cells, indicating that VEGFR-1 and Np-2 associate with each other. To perform the reciprocal experiment we have expressed in porcine aortic endothelial cells a Np-2 receptor containing an in-frame myc epitope at the C terminus. Surprisingly, the myc-tagged Np-2 receptor lost most of its VEGF(165) binding capacity but not its semaphorin-3F binding ability. Nevertheless, when Np-2myc was co-expressed in cells with VEGFR-1, it partially regained its VEGF(165) binding ability. Antibodies directed against the myc epitope co-immunoprecipitated (125)I-VEGF(165).Np-2myc and (125)I- VEGF(165).VEGFR-1 complexes from cells co-expressing VEGFR-1 and Np-2myc, indicating again that VEGFR-1 associates with Np-2. Our experiments therefore indicate that Np-2, and possibly also Np-1, associate with VEGFR-1 and that such complexes may be part of a cell membrane-associated signaling complex.
Assessment of credibility of some folk medicinal claims on Bombax ceiba L. (2014)

The present paper is an attempt to assess the credibility of various folk claims on medicinal uses of Bombax ceiba L. in view of scientific studies done so far. Almost all parts of the plant are widely used by tribal communities for gastrointestinal and skin diseases, gynecological and urino-genital disorders, general debility, diabetes and impotence. There are direct evidences of scientific validation for six pathological conditions while its anti-microbial, anti-inflammatory, antioxidant, analgesic and oxytocic potential provides indirect evidences for some of the folk medicinal claims and both these evidences authenticate the credibility of folk wisdom on B. ceiba L.
A Cooperative Role for the Counteranion in the PCl<sub>5</sub>-Initiated Living, Cationic Chain Growth Polycondensation of the Phosphoranimine Cl<sub>3</sub>P═NSiMe<sub>3</sub> (2012)

The counteranion associated with the cationic initiator [Cl(3)P═N═PCl(3)](+) ([4](+)) generated during the PCl(5)-initiated living, cationic chain growth polycondensation of the N-silylphosphoranimine Cl(3)P═NSiMe(3) (3) to give poly(dichlorophosphazene), [N═PCl(2)](n) (2), has been found to have a dramatic effect on the polymerization. When the counteranion of [4](+) was changed from PCl(6)(-) or Cl(-) to the weakly coordinating anions [BAr*(F)(4)](-) and [BAr(F)(4)](-) (Ar*(F) = 3,5-{CF(3)}(2)C(6)H(3), Ar(F) = C(6)F(5)) instead of the polymerization of 3 being complete in 4-6 h, no reaction was observed after 24 h. Remarkably, the polymerization of 3 may be initiated by Cl(-) anions even in the absence of an active cation such as [4](+). However, in the presence of [4](+), the reaction proceeded significantly faster and allowed for molecular weight control. These results reveal that the currently accepted mechanism for the PCl(5)-initiated living polymerization of 3 needs to be revised to reflect the key role of the counteranion present.
Coordinated targeting of cold and nicotinic receptors synergistically improves obesity and type 2 diabetes (2018)

Pharmacological stimulation of brown adipose tissue (BAT) thermogenesis to increase energy expenditure is progressively being pursued as a viable anti-obesity strategy. Here, we report that pharmacological activation of the cold receptor transient receptor potential cation channel subfamily M member 8 (TRPM8) with agonist icilin mimics the metabolic benefits of cold exposure. In diet-induced obese (DIO) mice, treatment with icilin enhances energy expenditure, and decreases body weight, without affecting food intake. To further potentiate the thermogenic action profile of icilin and add complementary anorexigenic mechanisms, we set out to identify pharmacological partners next to icilin. To that end, we specifically targeted nicotinic acetylcholine receptor (nAChR) subtype alpha3beta4 (α3β4), which we had recognized as a potential regulator of energy homeostasis and glucose metabolism. Combinatorial targeting of TRPM8 and nAChR α3β4 by icilin and dimethylphenylpiperazinium (DMPP) orchestrates synergistic anorexic and thermogenic pathways to reverse diet-induced obesity, dyslipidemia, and glucose intolerance in DIO mice.
Locations of the three primary binding sites for long-chain fatty acids on bovine serum albumin. (1991)

Binding of 13C-enriched oleic acid to bovine serum albumin and to three large proteolytic fragments of albumin--two complementary fragments corresponding to the two halves of albumin and one fragment corresponding to the carboxyl-terminal domain--yielded unique patterns of NMR resonances (chemical shifts and relative intensities) that were used to identify the locations of binding of the first 5 mol of oleic acid to the multidomain albumin molecule. The first 3 mol of oleic acid added to intact albumin generated three distinct NMR resonances as a result of simultaneous binding of oleic acid to three heterogeneous sites (primary sites). Two of these resonances were seen upon addition of 1 or 2 mol of oleic acid to fragments representing either the carboxyl-terminal half (residues 307-582) or the carboxyl-terminal domain (residues 377-582); the third resonance was seen upon addition of 1 mol of oleic acid to the fragment representing the amino-terminal half (residues 1-306). The resonance patterns for the fourth and fifth moles of oleic acid added to albumin (secondary sites) could not be duplicated by addition of more oleic acid to individual fragments. These resonance patterns were generated, however, when the two complementary fragments were mixed in equimolar proportions to form an albumin-like complex with a reconstituted middle domain. Thus, two primary fatty acid binding sites are assigned to the carboxyl-terminal domain, one primary site is assigned to the amino-terminal half, and the secondary sites are assigned to the middle domain. This distribution suggests albumin to be a less symmetrical binding molecule than theoretical models predict. This work also demonstrates the power of NMR for the study of microenvironments of individual fatty acid binding sites in specific domains.
Microchannel plate response to hard x-rays. [8-100 keV x-rays] (1977)

The current response and the pulse response of a microchannel plate, MCP, to x-rays incident at an angle of 45/sup 0/ in the energy range of 8 to 100 keV were measured. The x-ray detection efficiency, pulse height distributions, and sensitive depth of the MCP were determined from these data. Detection efficiency extrapolates to zero below 8 keV, and ranges between values of 1% and 2.6% above this energy, much higher than anticipated from previous measurements for oblique incidence below 6 keV. When compared to previous measurements at higher energies, it is concluded that MCP's have a relatively constant detection efficiency in the energy range 10 to 600 keV, which is a rather unique property as compared to other x-ray detectors. It was found that the shape of current response curves differs somewhat from the shape of efficiency curves, that pulse height distributions are proportional to pulse height to the -1.1 to -1.6 power, and that 90% of the current originates in the first 25 to 30% of the MCP thickness.