gebhart/patrepeval huggingface dataset:
Post‐translational modification of POU domain transcription factor Oct‐4 by SUMO‐1 (2007)

POU domain transcription factor Oct-4 plays a crucial role in maintaining self-renewal and pluripotency of embryonic stem (ES) cells in a concentration-dependent manner. However, the molecular mechanism controlling Oct-4 levels in ES cells remains largely unknown. To explore the molecular mechanism regulating Oct-4 function, we constructed a mouse ES cell cDNA library and performed yeast two-hybrid screening using the POU domain of Oct-4 as bait. Here, we present novel evidence for Oct-4 interaction with Ubc9, an E2 conjugation enzyme for SUMO modification, and its modification by SUMO-1. The SUMO acceptor site was identified at lysine residue 118. Importantly, disruption of Oct-4 sumoylation reduced Oct-4 protein stability and self-renewal capacity in ES cells. Interestingly, expression of cYes was found to reduce when Oct-4 sumoylation was disrupted or Oct-4 expression downregulated in ES cells. We further demonstrate that Oct-4 was recruited to the cYes promoter region, suggesting that cYes might be a novel downstream gene of Oct-4. Taken together, we first demonstrate the post-translational modification of endogenous Oct-4 by SUMO and the role of sumoylation in regulating Oct-4 protein stability and function. Our findings provide new evidence for the important role of post-translational modification in controlling Oct-4 function in ES cells.
Dose-Dependent Effects of Scopolamine on Nocturnal Growth Hormone Secretion in Normal Adult Men: Relation to<i>δ</i>-Sleep Changes* (1991)

To explore the sensitivity of nocturnal GH secretion to different degrees of cholinergic blockade, we investigated the effects of two doses of the muscarinic receptor antagonist scopolamine (SCOP; 3.0 and 6.0 micrograms/kg, im) and placebo, administered in a randomized fashion at 2300 h on three nights to eight normal male volunteers. Both doses of SCOP produced significant reductions in mean nocturnal GH concentration compared to the effects of the placebo; the higher dose of SCOP reduced GH to a greater degree than the lower dose, but this difference was not statistically significant (mean, 2.3 micrograms/L after 6 micrograms/kg vs. 3.0 micrograms/L after 3 micrograms/kg). Both SCOP doses significantly shifted GH secretion into later portions of the night, with a significantly greater delay observed after the larger dose. Similarly, a significant delay in the time of the GH rise was produced by SCOP. In contrast, the effects of both doses of SCOP on delta-sleep or sleep onset were small. These data confirm earlier reports demonstrating that cholinergic muscarinic input represents a potentially important source of regulation of nocturnal GH release and suggest that the magnitude of the reduction in GH and the extent of delay in the GH rise time may reflect quantitative differences in the degree of cholinergic blockade. These data are in agreement with recent studies suggesting that the timing of GH release need not be associated with delta-sleep per se.
Complexes of DNA Vaccines With Cationic, Antigenic Peptides Are Potent, Polyvalent CD8<sup>+</sup> T-Cell-Stimulating Immunogens (2006)

A priority in current vaccine research is the development of multivalent vaccines that support the efficient priming of long-lasting CD8(+) T-cell immunity. We developed a novel vaccination strategy that used synthetic, cationic (positively charged), and antigenic peptides complexed to negatively charged nucleic acids: antigenic, major histocompatibility complex-class I-binding epitopes fused with a cationic sequence derived from the HIV tat protein (tat50-57: KKRRQRRR) were mixed with nucleic acids (e.g., CpG-containing oligonucleotides) to quantitatively form peptide/nucleic acid complexes. The injection of these complexes efficiently primed long-lasting, specific CD8(+) T-cell immunity of high magnitude. This chapter describes a novel strategy to codeliver complexes of cationic/antigenic peptides bound to antigen-encoding plasmid DNA vaccines in a way that enhances the immunogenicity of both components for T cells.
Assessment of Brain Metastases with Dynamic Susceptibility-weighted Contrast-enhanced MR Imaging: Initial Results (2003)

The results suggest that dynamic susceptibility-weighted contrast-enhanced MR imaging is a useful method for the assessment of radiosurgically treated brain metastases. The implemented technique with determination of the arterial input function enables an absolute quantification of the regional CBV and prediction of tumor response.
A functional fault model for sequential machines (1992)

A fault model at the state transition level is proposed for finite state machines. In this model, a fault causes the destination state of a state transition to be faulty. Analysis shows that a test set that detects all single-state-transition (SST) faults will also detect most multiple-state-transition (MST) faults in practical finite state machines. The quality of the test set generated for SST faults is close to that of the sequences derived from the checking experiment. It is also shown that the upper bound of the length of the SST fault test is 2MN/sup 2/ for an N-state M-transition machine, while that of the checking sequence is exponential. An automatic test generation algorithm and a test generation system, FTG, based on the model show that the test set generated for SST faults achieves high single stuck-at-fault coverage as well as high transistor fault coverage for multilevel implementations of the machine.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A 57-to-64 GHz ultra-compact 0.027 mm<sup>2</sup> reflection type phase shifter with low insertion loss (2014)

An ultra-compact reflection-type phase shifter (RTPS) with more than 190° continuous phase shift and low insertion loss using 90-nm CMOS process is demonstrated in this paper. Two circuit implementation techniques are employed: LC-type reflection loads with switches and 3-dB quadrature coupler. First, the LC-type reflection loads with switches have been proposed to alleviate the issue of quality factor. Second, the 3-dB quadrature coupler is designed with broadside couple to reduce chip size and enhance couple factor. Measurements show signal losses of 9.3dB±1.5dB including pad loss, loss flatness of ±0.9 dB, and a more than 190° continuously tunable range across 57-64 GHz with 0-mW dc power consumption. To the best of our knowledge, the proposed phase shifter has the smallest die size of 0.027 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , among all reported V-band CMOS phase shifter, which is important for a multi-way phased-array system.
Alternative techniques for measuring nutrient digestion in ruminants. (1997)

Because in vivo measurement of nutrient digestion in the rumen and small intestine requires ruminally and intestinally cannulated animals that are expensive, labor-intensive, and subject to error associated with markers and inherent animal variation, alternative techniques have been developed. Researchers have proposed various in situ or in vitro procedures for estimating ruminal and small intestinal nutrient digestion. This review summarizes these alternative techniques.
The Use of Active Controls to Augment Rotor/Fuselage Stability (1985)

The use of active blade pitch control to increase helicopter rotor/body damping is studied. Control is introduced through a conventional nonrotating swashplate. State variable feedback of rotor and body states is used. Feedback parameters include cyclic rotor flap and lead-lag states, and body pitch and roll rotations. The use of position, rate, and acceleration feedback is studied for the various state variables. In particular, the influence of the closed loop feedback gain and phase on system stability is investigated. For the rotor/body configuration analyzed, rotor cyclic inplane motion and body roll-rate and roll-acceleration feedback can considerably augment system damping levels and eliminate ground resonance instabilities. Scheduling of the feedback state, phase, and gain with rotor rotation speed can be used to maximize the damping augmentation. This increase in lead-lag damping can be accomplished without altering any of the system modal frequencies. Investigating various rotor design parameters (effective hinge offset, blade precone, blade flap stiffness) indicates that active control for augmenting rotor/body damping will be particularly powerful for hingeless and bearingless rotor hubs.
FETAL DEATH AFTER EXPOSURE TO METHYLENE BLUE DYE DURING MID-TRIMESTER AMNIOCENTESIS IN TWIN PREGNANCY (1996)

Methylene blue dye use during mid-trimester amniocentesis in twin pregnancy is associated with a high risk of small intestinal atresia. It is plausible that the effects of methylene blue as a fetotoxic agent may also lead to fetal death. We conducted a retrospective cohort study of all women who had an amniocentesis during twin pregnancy from 1980 through 1991 in New South Wales, Australia. Women who were exposed to methylene blue dye during the procedure were compared with women who had amniocentesis without dye exposure. Fetal death occurred in 31.8 per cent of pregnancies that had exposure to a high concentration of methylene blue, compared with 14.5 per cent of pregnancies exposed to a low concentration and 4.3 per cent of pregnancies with no exposure to dye. The unadjusted and adjusted risks and 95 per cent confidence intervals (CIs) for fetal death after any exposure to dye were 5.03 (2.12-11.91) and 8.52 (2.28-31.80), respectively. The adjusted odds ratio and 95 per cent CIs for the low and high concentration dye solutions were 4.63 (0.93-23.13) and 14.98 (3.40-66.08), respectively (chi-squared test for trend P < 0.001). Fetus papyraceous was significantly more likely among pregnancies exposed to a high concentration of methylene blue (P < 0.001) than among unexposed pregnancies. These results support the hypothesis that methylene blue dye use during mid-trimester amniocentesis in twin pregnancy increases the risk of fetal death.
Apraclonidine (1988)

We performed a double-masked, cross-over, dose-response study of apraclonidine hydrochloride (formerly known as ALO 2145) in 20 patients with elevated intraocular pressure (IOP). We administered three concentrations of apraclonidine (0.125%, 0.25%, 0.5%) and vehicle alone bilaterally every 12 hours for one week. Patients were examined 2, 5, and 8 hours after the initial dose, and then on day 2 and day 8. We studied IOP, pupillary diameter, interpalpebral fissure width, blood pressure, and pulse. There was a two-week washout period after each one-week session. All concentrations of apraclonidine significantly lowered IOP. The 0.5% and 0.25% concentrations had equal maximal effects, lowering IOP in each patient by an average of 27% relative to vehicle alone. This corresponded to a mean decrease in IOP of 8.7 mm Hg, from a baseline of 24.9 mm Hg to 16.2 mm Hg. The 0.5% and 0.25% concentrations were significantly more effective than the 0.125% concentration at two and eight hours. Mean interpalpebral fissure width increased in a dose-dependent fashion; the pupillary effect was minimal. Blood pressure and pulse were unchanged. Thirty percent of subjects reported transient dry nose or dry mouth. These symptoms may be dose-dependent.
Adaptive FEC on a reconfigurable processor for wireless multimedia communications (2002)

Due to variations in environmental conditions, many computationally intensive algorithms that need to be processed in a compact form factor often require a more flexible solution than a specialized ASIC. A ReConfigurable Processor Board (RCP) was jointly developed by a University of California San Diego and L3 Communications Corporation for implementing Digital Signal Processing (DSP) algorithms for wireless multimedia communications. The flexible architecture of the RCP makes it ideal for performing parallel tasks and can process multiple algorithms concurrently. As an application example, an Adaptive Viterbi Decoder (AVD) was designed and implemented on the RCP. The AVD can adapt itself to varying channel characteristics and service requirements at run time. It is capable of processing data with constraint lengths from 7 to 15 and code rates form 1/2 to 1/6. The AVD provides robust decoding for video, audio and data communication channels.
Generation of electronic program guide for RF fed TV channels by recognizing the channel logo using fuzzy multifactor analysis (2010)

This paper presents a novel method for providing the Electronic Program Guide (EPG) for the Radio Frequency (RF) fed cable TV users by recognizing the channel logo and then extracting the EPG from their website. The prototype is developed in X86 platform and then ported on a real time DSP platform and performs with nearly 100% accuracy.
The electronic clinical brain atlas : multiplanar navigation of the human brain ; based on the classic Talairach-Tournoux and Schaltenbrand brain atlases (1997)

This title integrates several landmark atlases as well as magnetic resonance scans into a multi-purpose, multi-dimensional, interactive CD-ROM. The print atlases incorporated are: Co-Planar Sterotaxic of the Human Brain and Referentially Oriented Cerebral MRI Anatomy, both by J. Talairach and J. Tournoux, and Atlas for Stereotaxy of the Human Brain by G. Schaltenbrand and W. Wahren. The information contained in these print atlases has been digitized, pre-processed, enhanced and fully labelled. The CD-ROM offers several interactive features: All atlases are co-registered; interactive atlas, MR and CT registration and overlay; 3D animations of cerebral structures; navigation through the atlases and images in 3D sterotactic space; importing of atlas images with user annotations to other applications; three anatomical indices containing 600 structures and a selected glossary of structures promoting ease of use (allowing the user to choose any object from the anatomical index, find the corresponding object in MRI data, and visualize, manipulate and quantify in two and three dimensions). The minimum system requirements for PCs are: a 486 Windows-compatible computer, at least 8MB of RAM, a double-speed CD-ROM drive, a 256-colour display or better, Windows 3.1 or higher and DOS 3.3 or higher. Recommended system configuration: Pentium with 32MB RAM, 256-colour display, 11MB space available, Windows 95 and quad-speed CD-ROM drive. The minimum system requirements for Apple Macintosh computers are: 13 colour display, at least 9MB RAM, 256-colour display and a double-speed CD-ROM drive. Recommended system configuration: Power Macintosh with at least 8MB of RAM, System 7, a 13 or larger colour display, 256-colour display, approximately 11MB of space available, and a quad-speed CD-ROM drive.
High transport<i>J</i><sub>c</sub>in magnetic fields up to 28 T of stainless steel/Ag double sheathed Ba122 tapes fabricated by scalable rolling process (2014)

The recently discovered iron-based superconductors with very high upper critical field and small anisotropy have been regarded as a potential candidate material for high field applications. However, enhancements of superconducting properties are still needed to boost the successful use of iron-based superconductors in such applications. Here, we propose a new sheath architecture of stainless steel (SS)/Ag double sheath and investigate its influence on the microstructures and Jc-H property. We found that the transport Jc-H curves for rolled and pressed tapes both show extremely small magnetic field dependence and exceed 3*10^4A/cm^2 under 28 T, which are much higher than those of low-temperature superconductors. More interestingly, 12 cm long rolled tape shows very high homogeneity and sustains Jc as high as 7.7*10^4 A/cm^2 at 10 T. These are the highest values reported so far for iron-based superconducting wires fabricated by scalable rolling process. The microstructure investigations indicate that such high Jc was achieved by higher density of the core and uniform deformation resulting better texturing. These results indicate that our process is very promising for fabricating long Ba122 wires for high field magnet, i.e. above 20 T.
Synthesis of Heterocycles via Palladium π-Olefin and π-Alkyne Chemistry (2004)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Heterocycles via Palladium π-Olefin and π-Alkyne ChemistryGilson Zeni and Richard C. LarockView Author Information Department of Chemistry, Iowa State University, Ames, Iowa 50011 Cite this: Chem. Rev. 2004, 104, 5, 2285–2310Publication Date (Web):February 20, 2004Publication History Received30 September 2003Published online20 February 2004Published inissue 1 May 2004https://pubs.acs.org/doi/10.1021/cr020085hhttps://doi.org/10.1021/cr020085hresearch-articleACS PublicationsCopyright © 2004 American Chemical SocietyRequest reuse permissionsArticle Views10045Altmetric-Citations1070LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Catalysts,Cyclization,Heterocyclic compounds,Hydrocarbons,Palladium Get e-Alerts
Artificial compound eye inspired by imaging principle of Xenos peckii (2015)

This work reports an artificial compound eye inspired by the imaging principle of Xenos peckii, which is an endoparasite of paper wasps. The unique eye design exhibits higher spatial resolution and better sensitivity than conventional compound eyes. The biomimetic compound eye comprises three layers, a microprism arrays, a microlens arrays and an aperture arrays. All of the layers were formed on a planar substrate, the device can be directly integrated with a commercial image sensor. Each channel detects a different part of the whole scene, and the partial images are stitched in the following image processing step. The proposed artificial compound eye can create great opportunities for applications in medical, industrial and military fields.
Characterization of Adrenoceptor Involvement in Skeletal and Cardiac Myotoxicity Induced by Sympathomimetic Agents: Toward a New Bioassay for β-blockers (2003)

Excessive levels of catecholamines have long been known to be cardiotoxic, but less well known are their toxic effects on skeletal muscle. By using an antimyosin monoclonal antibody and quantitative methods to measure the extent of myocyte necrosis, and by employing modulators of adrenoceptors (ARs), including clenbuterol, bupranolol, propranolol, bisoprolol, atenolol, ICI-118551, phenoxybenzamine, prazosin, and yohimbine, the involvement of ARs in isoproterenol-induced myotoxicity was characterized. In the myocardium, the toxic effects were predominantly mediated via the beta(1)-ARs. In the soleus muscle, it was almost solely via the beta(2)-ARs. Myotoxicity was also observed in the myocardium when challenged with the beta(2)-AR agonist clenbuterol. This was found to be mediated via sympathetic presynaptic beta(2)-ARs, leading to enhanced release of norepinephrine. This effect was abolished by prior treatment with reserpine. The skeletal muscle was found to be more sensitive to the myotoxic effects than cardiac muscle at lower doses of beta-AR agonists. These experiments introduce a new way of assaying beta-AR antagonists by classifying them according to their ability to prevent catecholamine-induced myotoxicity. Further research along these lines may deepen understanding of which beta-blockers work best in heart failure therapy.
Cell-specific expression of the macrophage scavenger receptor gene is dependent on PU.1 and a composite AP-1/ets motif. (1994)

The type I and II scavenger receptors (SRs) are highly restricted to cells of monocyte origin and become maximally expressed during the process of monocyte-to-macrophage differentiation. In this report, we present evidence that SR genomic sequences from -245 to +46 bp relative to the major transcriptional start site were sufficient to confer preferential expression of a reporter gene to cells of monocyte and macrophage origin. This profile of expression resulted from the combinatorial actions of multiple positive and negative regulatory elements. Positive transcriptional control was primarily determined by two elements, located 181 and 46 bp upstream of the major transcriptional start site. Transcriptional control via the -181 element was mediated by PU.1/Spi-1, a macrophage and B-cell-specific transcription factor that is a member of the ets domain gene family. Intriguingly, the -181 element represented a relatively low-affinity binding site for Spi-B, a closely related member of the ets domain family that has been shown to bind with relatively high affinity to other PU.1/Spi-1 binding sites. These observations support the idea that PU.1/Spi-1 and Spi-B regulate overlapping but nonidentical sets of genes. The -46 element represented a composite binding site for a distinct set of ets domain proteins that were preferentially expressed in monocyte and macrophage cell lines and that formed ternary complexes with members of the AP-1 gene family. In concert, these observations suggest a model for how interactions between cell-specific and more generally expressed transcription factors function to dictate the appropriate temporal and cell-specific patterns of SR expression during the process of macrophage differentiation.
Focusing fluids and light (2008)

There exist two major technical challenges when developing on-chip single- particle detection systems: focusing the particles and focusing the light. Flow cytometry is used to measure the optical and fluorescence characteristics of single cells while cells pass through an optical interrogation area (with dimensions on the order of several micrometers to tens of micrometers) that is defined by a focused laser beam.
Thermoplasmonic Membrane Distillation (2017)

Membrane Distillation (MD) is a hybrid thermal/membrane technology emerging either as a promising alternative or as a complement to Reverse Osmosis, having the potential to concentrate saline solutions even up to supersaturation. Presently, performance of conventional MD systems is drastically affected by temperature polarization, a phenomenon intrinsically connected to the removal of latent heat due to evaporation, which causes the decrease of feed temperature at the membrane surface with respect to the bulk. As a consequence, the net driving force to mass transfer falls down along with the thermal efficiency of the process. Due to these adverse effects, technological applications of MD are still elusive. In this work, we prove that thermoplasmonic effect induced by photothermal excitations of silver nanoparticles (Ag NPs), incorporated into polyvinylidene (PVDF) membranes, remarkably increase the feed temperature at the membrane surface exposed to light radiation, thus achieving unmatched performance in a vacuum MD unit.