gebhart/patrepeval huggingface dataset:
Effect of L-2286, a Poly(ADP-ribose)polymerase Inhibitor and Enalapril on Myocardial Remodeling and Heart Failure (2008)

Increased activation of poly(ADP-ribose) polymerase (PARP) enzyme has been implicated in the pathogenesis of acute and chronic myocardial dysfunction. We have demonstrated the protective effect of PARP inhibitors against postinfarction myocardial remodeling and heart failure. The primary aim of our recent work was to compare the effect and efficacy of a potent PARP-inhibitor (L-2286) to enalapril, a widely used angiotensin-converting enzyme (ACE) inhibitor. in experimental heart failure model. Both L-2286 and enalapril were tested in a rat model of chronic heart failure after isoproterenol-induced myocardial infarction. After a 12-week treatment period, echocardiography was performed, cardiac hypertrophy and interstitial collagen deposition were assessed, and the phosphorylation state of Akt-1/GSK-3beta pathway as well as the PKC and MAPK kinases were determined. Both PARP and ACE inhibition reduced the progression of postinfarction heart failure by attenuating cardiac hypertrophy and interstitial fibrosis. More importantly, PARP inhibition increased the activity of the prosurvival signal transduction factors (Akt-1/GSK-3beta pathway, PKCepsilon). Due to these effects, L-2286 improved the systolic left ventricular function. Enalapril treatment exerted a similar, but weaker protective effect against postinfarction myocardial remodeling and heart failure. In conclusion, we demonstrated in an experimental heart failure model that L-2286 decreased the postinfarction myocardial remodeling more effectively than enalapril treatment.
Efficient visualization of 3D medical scenes for remote interactive applications (2003)

In this paper, we propose a software framework for developing interactive medical applications that execute on remote servers. The 3D visualization interface of the application can be disconnected from the computation core to allow rendering of the medical scene on the user display while the heavy computations are executed on a remote workstation or a computational grid. We introduce a software architecture designed for reducing the communication overhead between the remote and the local processes and for easing software development by offering transparent management of communications. Execution of a 2D and a 3D segmentation applications are demonstrated and performances are evaluated.
Vehicular mounted repeater in emergency medical services (EMS) communications (1979)

The operation of vehicular mounted repeaters as used in emergency medical services (EMS) on the UHF MED channels is discussed. Advantages and disadvantages are examined critically as well as the compromises. The effect of this concept on the shared use of MED channels and mutual aid is explored in detail. Both one-way and two-way repeat concepts are covered. The intent of the FCC rules affected by vehicular mounted repeaters is carefully considered. The author demonstrates that the vehicular mounted repeater is not merely a piece of equipment but a very different system concept, and therefore must be considered a major decision by the system designers.
Activation of a p38 mitogen-activated protein kinase in human neutrophils by lipopolysaccharide (1996)

Stimulation of human neutrophils by LPS is central to the pathogenesis of sepsis and the adult respiratory distress syndrome. The intracellular signaling pathway that results in cellular responses following LPS stimulation in neutrophils is unknown. We report that exposure of neutrophils to LPS results in the phosphorylation and activation of a p38 mitogen-activated protein (MAP) kinase, occurring in a concentration-dependent manner, with maximum response at 20 to 25 min. Partial purification of a p38 MAP kinase by ion exchange chromatography established it as distinct from the p42/p44 (extracellular signal-regulated kinases (ERK-1 and ERK-2) MAP kinases). Activation of the p38 MAP kinase by LPS in human neutrophils occurs via CD14, a proposed LPS receptor, and requires the presence of plasma containing the LPS-binding protein. This intracellular signaling pathway is independent of protein kinase C and does not involve Raf, MAP/ERK kinase kinase-1, MAP/ERK kinase-1, or MAP/ERK kinase-2 and does not result in the activation of the p42/p44 ERK MAP kinases or the c-jun N-terminal kinases.
Identification of fatty acid hydroperoxide cofactors in the cytochrome P450-mediated oxidation of estrogens to quinone metabolites. Role and balance of lipid peroxides during estrogen-induced carcinogenesis. (1994)

Lipid hydroperoxide-supported metabolic redox cycling between diethylstilbestrol (DES) or catechol-estrogens and their corresponding quinones has been postulated previously to play a role in estrogen-induced toxicity and carcinogenesis. As part of an examination of this postulate, we investigated (a) the effectiveness of various lipid hydroperoxides as oxidants in microsome-mediated quinone formation, and (b) the dependence of DES oxidation to quinone on lipid hydroperoxide levels in liver and kidney of hamsters. Kinetic analyses of the lipid hydroperoxide-mediated conversion of DES to DES quinone revealed that of the peroxides tested (9Z,11E,13(S))-13-hydroperoxyoctadecadien-1-oic acid was the most powerful oxidant (Vmax = 10.5 nmol min-1; Km = 21.3 microM) followed by (5(S),6E,8Z,11Z,14Z)-5-hydroperoxyeicosatetra en-1-oic acid (Vmax = 1.0 nmol min-1; Km 10.0 microM). The other pure fatty acid hydroperoxide isomers or mixtures had a lower affinity for the enzyme (Km values ranging from 23.1 to 130 microM) and/or lower maximal velocity (Vmax values ranging from 0.2 to 7.0 nmol min-1). Levels of Z,Z-dienestrol, which arises from spontaneous rearrangement of DES quinone, were not affected in liver of hamsters treated with estradiol implants for 9 days and an injection of 20 mg/kg DES but increased in kidney by 120% over levels observed in animals without implants (5.5 and 12.1 nmol/g of wet tissue, respectively). Z,Z-Dienestrol concentrations correlated directly with lipid hydroperoxide concentrations in liver and kidney of control and estradiol-treated hamsters. The increased conversion of DES to quinone in kidney compared with that in liver was catalyzed by comparable activities of cytochrome P450 IA, the enzyme family catalyzing this oxidation. These results demonstrated that the oxidation of DES to quinone was directly dependent on the lipid hydroperoxide levels in the organ of metabolic conversion in addition to the enzyme activity catalyzing this reaction. The elevated lipid hydroperoxide levels in kidney of estrogen-treated hamsters indicate enhanced estrogen quinone formation and therefore enhanced generation of free radicals by metabolic redox cycling of estrogens in this organ prior to the appearance of malignancy.
The Use of Dermal Autograft as an Adjunct to Breast Reconstruction with Tissue Expanders (2012)

Therapeutic, IV.
Application of laser-accelerated protons to the demonstration of DNA double-strand breaks in human cancer cells (2009)

We report the demonstrated irradiation effect of laser-accelerated protons on human cancer cells. In vitro (living) A549 cells are irradiated with quasimonoenergetic proton bunches of 0.8–2.4 MeV with a single bunch duration of 15 ns. Irradiation with the proton dose of 20 Gy results in a distinct formation of γ-H2AX foci as an indicator of DNA double-strand breaks generated in the cancer cells. This is a pioneering result that points to future investigations of the radiobiological effects of laser-driven ion beams. Unique high-current and short-bunch features make laser-driven proton bunches an excitation source for time-resolved determination of radical yields.
A study of the polarization properties of a rectangular polarization-maintaining fiber (1989)

An experimental study of the polarization properties of a rectangular polarization-maintaining (PM) fiber is reported. The extinction ratio is measured at 1320 nm for lengths of 20, 40, 100, 310, and 804 m. The length dependence of the extinction ratio is demonstrated to be in excellent agreement with the predictions of a model derived from random coupling theory. This agreement is due to the careful winding and packaging of the rectangular PM fiber, whereby minimal external perturbations are introduced. This is facilitated by the rectangular geometry of the fiber. The polarization-holding parameter is 2.08+or-0.09*10/sup -6/ m/sup -1/.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Distribution, Levels, and Activity of Glycogen Synthase Kinase-3 in the Alzheimer Disease Brain (1997)

A number of studies have implicated a proline-directed protein kinase, glycogen synthase kinase-3 (GSK-3) in the hyperphosphorylation of tau in Alzheimer's disease (AD). Toward understanding the role of GSK-3 in the abnormal hyperphosphorylation of tau in AD we have found that GSK-3 is prominently present in neuronal cell bodies and their processes and co-localizes with neurofibrillary changes in AD brain. Furthermore, the levels of GSK-3 as determined by indirect ELISA are approximately 50% increased in the postsynaptosomal supernatant from AD brains as compared to the controls. However, no increase in GSK-3 enzyme activity was detected. In AD brain, with its reduced phosphatase activity, even normal levels of GSK-3 activity might be sufficient for the hyperphosphorylation of tau.
Polyethylene-glycol-Stabilized Ag Nanoparticles for Surface-Enhanced Raman Scattering Spectroscopy: Ag Surface Accessibility Studied Using Metalation of Free-Base Porphyrins (2014)

Silver nanoparticles (Ag NPs) stabilized with a permeable layer of long-chain polyethylene glycol (PEG) represent a new generation of surface-enhanced Raman scattering (SERS) substrates for bioanalytical applications. This paper will shed light on their efficiency in the SERS detection of biomolecules. Two types of thiol-terminated PEG (average molecular weight 5000 g/mol) were used. Metalation of free-base porphyrins (10–9–10–6 M) of different size and charge was employed as the probe of accessibility of the Ag-PEG NP surface. The influence of NaCl (0.01 M) on the system was examined. The metalation was significantly decreased by the interaction of porphyrins with propionylamino groups of PEG1, while no interaction with the neutral chain of PEG2 was observed. Sterical hindrance of the porphyrin side groups seemed to cause a rather perpendicular porphyrin orientation on both types of Ag-PEG NP surfaces, inhibiting the metalation. Moreover, chloride anions influenced the structure of the polymer coating and improved the porphyrin accessibility on the Ag-PEG1 NPs. Finally, the studied Ag-PEG NPs remained isolated even after addition of porphyrins and chlorides, therefore being favorable for SERS applications inside the living cells.
[Whole sequence analysis of brain-derived neurotrophic factor gene in Asiatic Black Bear through faecal sampling]. (2006)

Using our lab's improved protocol for faecal DNA extraction, the entire 753-bp DNA coding sequence of the nuclear brain-derived neurotrophic factor (BDNF) gene was cloned for the first time from Asiatic Black Bear Selenarctos thibetanus faecal samples with primers based on the reported sequence of the Malayan Bear BDNF gene. Hair was used as a positive control and the experiments were repeated several times to obtain reliable and identical results. Sequence analysis showed that the BDNF gene of Asiatic Black Bear was highly conserved compared to those of human and giant panda, with an identity of 94.5% and 98.9%, respectively. The deduced amino acid sequence of the mature protein was found to be identical to those of all the reported mammalians. According to gene sequence alignment, the giant panda appeared to be phylogenetically closer to Asiatic Black Bear than the lesser panda. This study represents the first time that a non-invasive method such as faecal sampling was used to analyze a functional nuclear BDNF gene of Asiatic Black Bear. It will not only provide important reference for the conservation and breeding of Asiatic Black Bear and open up new avenues of non-invasive sampling in the study of endangered wildlife, but also provide another molecular evidence for the study of relationship of Asiatic Black Bear and its related species.
HIV-1 protease inhibitors decrease proliferation and induce differentiation of human myelocytic leukemia cells (2000)

Inhibitors of the protease of human immunodeficiency virus type 1 (HIV-1) may inhibit cytoplasmic retinoic acid-binding proteins, cytochrome P450 isoforms, as well as P-glycoproteins. These features of the protease inhibitors might enhance the activity of retinoids. To explore this hypothesis, myeloid leukemia cells were cultured with all-trans retinoic acid (ATRA) either alone or in combination with the HIV-1 protease inhibitors indinavir, ritonavir, and saquinavir. Consistent with the hypothesis, the HIV-1 protease inhibitors enhanced the ability of ATRA to inhibit growth and induce differentiation of HL-60 and NB4 myeloid leukemia cells, as measured by expression of CD11b and CD66b cell surface antigens, as well as reduction of nitroblue tetrazolium. Growth of ATRA-resistant UF-1 cells was also inhibited when cultured with the combination of ATRA and indinavir. Moreover, indinavir enhanced the ability of ATRA to induce expression of the myeloid differentiation-related transcription factor C/EBPepsilon messenger RNA in NB4 cells by 9.5-fold. Taken together, the results show that HIV-1 protease inhibitors enhance the antiproliferative and differentiating effects of ATRA on myeloid leukemia cells. An HIV-1 protease inhibitor might be a useful adjuvant with ATRA for patients with acute promyelocytic leukemia and possibly retinoid-resistant cancers.
Evaluation of a commercial ELISA for diagnosis of paratuberculosis in cattle (2001)

Results obtained with use of the new ELISA agreed favorably with those of a previous ELISA. Practitioners must be aware of variability in the sensitivity of the test, which depends on the clinical and shedding status of the cattle, because this may affect interpretation of test results.
Aripiprazole: Initial Clinical Experience with 142 Hospitalized Psychiatric Patients (2005)

Aripiprazole was used in a range of disorders and was generally well tolerated. Adverse effects may reflect its unique dopamine partial-agonist activity. Since aripiprazole is likely to be considered for obese patients, body weight should be considered in establishing adequate doses. Controlled trials of this antipsychotic agent in disorders other than schizophrenia are needed.
Surface Acoustic Waves in Reverse-Biased AlGaN/GaN Heterostructures (2008)

Properties of surface acoustic waves (SAWs) in reverse-biased AlGaN/GaN heterostructures on (0001) sapphire substrates were studied by examining the characteristics of SAW filters composed of interdigital Schottky and ohmic contacts. The fundamental and higher frequency SAW signals in measured -parameters were attributed to Rayleigh and Sezawa modes, respectively. The onsets of the SAW signals, which were close to the threshold voltage of HEMTs in the vicinities of the respective filters, changed in response to the spatial variation of the threshold voltage. The onset of Sezawa mode was deeper than that of Rayleigh mode, and the difference in onset was larger for longer SAW wavelengths. These results are possibly explained by the change of the input capacitance of interdigital transducers due to the reverse-bias voltages or by the difference in the distribution of SAW energy between the two modes.
Compatibility characterization of poly(lactic acid)/poly(propylene carbonate) blends (2005)

Abstract The compatibility of poly(lactic acid) (PLA)/poly(propylene carbonate) (PPC) blends was investigated with Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and tensile testing. The PLA/PPC blends were prepared over the whole composition range. FTIR spectroscopy revealed that there were several specific interactions between the chains of PLA and PPC: the interaction between CH and OC and CO···OC or CO···OC dipole–dipole interactions. Moreover, PLA and PPC were compatible. DSC indicated that PLA and PPC were partially miscible but compatible to some extent because of the similar chemical natures of the blend components. TGA showed that the compatibility of PLA and PPC enhanced the thermal stability of PPC in the blends. As calculated by the Horowitz–Metzger equation, the activation energy for decomposition ( E t ) of PPC in PLA/PPC (70/30) was 200.6 kJ/mol, whereas E t of pure PPC was only 56.0 kJ/mol. A study of the mechanical properties versus the composition and the strain versus the stress illustrated that there was good compatibility between PLA and PPC, and the phase inversion of the PLA/PPC system occurred between 70 and 60 wt % PLA in the PLA/PPC blends. The Pukanszky model gave credit to very strong interfacial adhesion between PLA and PPC. © 2005 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 94–101, 2006
Analysis of Gas-Lubricated Foil Journal Bearings (1983)

This work is concerned with an evaluation of the performance of a gas journal bearing using a spring supported compliant foil as the bearing surface. The analysis, conducted for both single and multipad configurations, is concerned with the effects that the various structural, geometric, and operational variables have on bearing behavior. Following the solution of the relevant differential equation, tabular or graphical solutions are provided for a range of relevant geometric and operational parameters. The solutions include values of the colinear and cross-coupled spring coefficients due to both structural and hydrodynamic stiffness. Desirable design features with regard to start of bearing arc, selection of load angle, number of pads and degree of compliance are discussed.
Assessing the reliability of calculated catalytic ammonia synthesis rates (2014)

We introduce a general method for estimating the uncertainty in calculated materials properties based on density functional theory calculations. We illustrate the approach for a calculation of the catalytic rate of ammonia synthesis over a range of transition-metal catalysts. The correlation between errors in density functional theory calculations is shown to play an important role in reducing the predicted error on calculated rates. Uncertainties depend strongly on reaction conditions and catalyst material, and the relative rates between different catalysts are considerably better described than the absolute rates. We introduce an approach for incorporating uncertainty when searching for improved catalysts by evaluating the probability that a given catalyst is better than a known standard.
Isolation and Characterization of Adult Stem Cells from Human Salivary Glands (2008)

Currently, adult stem cells are attracting significant interest in regenerative medicine and tissue engineering. These cells have been isolated from various tissue sources; however, in most cases, adult stem cells useful for tissue engineering and regeneration are present at a low frequency. High numbers of stem cells with an effective and reliable potential for differentiation are needed for clinical applications. Thus, the identification of new stem cell sources and the establishment of optimized cell culture conditions that allow for the amplification of stem cells are of utmost relevance. In addition, the isolation procedure should ideally be minimally invasive and possibly be performed under local anesthesia. We report here for the first time on the identification of adult stem cells with mesenchymal characteristics in human parotid gland tissue. Cells were isolated from freshly resected specimens of parotid glands using enzymatic digestion and plastic adhesion protocols. Following an initial proliferation period and short-term culture for four passages, immunophenotyping revealed the presence of mesenchymal stem cell markers. In the presence of tissue-specificinduction medium, stem cells could be differentiated into adipogenic, osteogenic, and chondrogenic cell types. Tissue-specific differentiation was confirmed by histochemical and immunocytochemical staining as well as by RT-PCR for defined marker genes. This study is, to the best of our knowledge, the first report on the isolation and differentiation of stem cells from adult human parotid glands. Although isolated from an endodermal tissue source, these stem cells share many characteristics with MSCs. Easy accessibility and a high differentiation potential make salivary gland-derived stem cells a promising source for future applications in regenerative medicine.
Semen Collection by Electrical Stimulation (1955)

Frequently bulls are encountered that refuse or are unable to serve a female or .an artificial vagina. Massage of the internal genitalia has been found to be an unsatisfactory way of obtaining semen. Semen collection by electroejaculation has been demonstrated in rams, boars and bulls. Failure of a young Guernsey bull at the Iowa State College Dairy Farm to serve a female or an artificial vagina prompted the development of a unit to collect semen by electrical stimulus. The instrument was designed by the Instrument Shop at Iowa State College.
Great thermoelectric power factor enhancement of CoSb3 through the lightest metal element filling (2011)

Lithium, the lightest metal element with a small ionic radius, is successfully filled into the voids of CoSb3 by utilizing the high pressure synthesis technique. The synthesized Li0.4Co4Sb12 shows the largest thermoelectric power factor of 6000 μW m−1 K−2 among all elemental filled CoSb3 materials. This significantly enhanced thermoelectric power factor is attributed to the large carrier mobility of Li0.4Co4Sb12, 61 cm2 V−1 s−1, featuring a good electron crystal property for the Li-filled CoSb3 samples.
Cutting Edge: Differential Constitutive Expression of Functional Receptors for Lysophosphatidic Acid by Human Blood Lymphocytes (2000)

Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) from platelets and macrophages mediate T cell functions. Endothelial differentiation gene-encoded G protein-coupled receptors (Edg Rs) are specific for S1P (Edg-1, -3, -5, and -8 Rs) and LPA (Edg-2, -4, and -7 Rs). Human T cell tumors express many Edg Rs for both LPA and S1P. In contrast, human blood CD4+ T cells express predominantly Edg-4, and CD8+ T cells show only traces of Edg-2 and -5, by quantification of mRNA and Edg R Ags. LPA at 10-10-10-6 M suppressed significantly the secretion of IL-2 from anti-CD3 plus anti-CD28 Ab-challenged CD4+ T cells, but not CD8+ T cells. Monoclonal anti-Edg-4 R Ab, like LPA, suppressed stimulated IL-2 secretion from CD4+ T cells, but not CD8+ T cells. Constitutive expression of Edg-4 by CD4+, but not CD8+, human T cells accounts for differential functional responsiveness of the T cell subsets to LPA.