gebhart/patrepeval huggingface dataset:
Tumor Dormancy and Interplay with Hypoxic Tumor Microenvironment (2019)

The tumor microenvironment is a key factor in disease progression, local resistance, immune-escaping, and metastasis. The rapid proliferation of tumor cells and the aberrant structure of the blood vessels within tumors result in a marked heterogeneity in the perfusion of the tumor tissue with regions of hypoxia. Although most of the tumor cells die in these hypoxic conditions, a part of them can adapt and survive for many days or months in a dormant state. Dormant tumor cells are characterized by cell cycle arrest in G0/G1 phase as well as a low metabolism, and are refractive to common chemotherapy, giving rise to metastasis. Despite these features, the cells retain their ability to proliferate when conditions improve. An understanding of the regulatory machinery of tumor dormancy is essential for identifying early cancer biomarkers and could provide a rationale for the development of novel agents to target dormant tumor cell populations. In this review, we examine the current knowledge of the mechanisms allowing tumor dormancy and discuss the crucial role of the hypoxic microenvironment in this process.
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution (1999)

The three-dimensional structure of the catalytic core of the family 6 cellobiohydrolase II, Cel6A (CBH II), from Humicola insolens has been determined by X-ray crystallography at a resolution of 1.92 Å. The structure was solved by molecular replacement using the homologous Trichoderma reesei CBH II as a search model. The H. insolens enzyme displays a high degree of structural similarity with its T. reeseiequivalent. The structure features both O- (α-linked mannose) and N-linked glycosylation and a hexa-co-ordinate Mg2+ ion. The active-site residues are located within the enclosed tunnel that is typical for cellobiohydrolase enzymes and which may permit a processive hydrolysis of the cellulose substrate. The close structural similarity between the two enzymes implies that kinetics and chain-end specificity experiments performed on the H. insolens enzyme are likely to be applicable to the homologous T. reesei enzyme. These cast doubt on the description of cellobiohydrolases as exo-enzymes since they demonstrated that Cel6A (CBH II) shows no requirement for non-reducing chain-ends, as had been presumed. There is no crystallographic evidence in the present structure to support a mechanism involving loop opening, yet preliminary modelling experiments suggest that the active-site tunnel of Cel6A (CBH II) is too narrow to permit entry of a fluorescenyl-derivatized substrate, known to be a viable substrate for this enzyme.Co-ordinates for the structure described in this paper have been deposited with the Brookhaven Protein Data Bank with accession code 1BVW.PDB.
Influence of chemical doping on the performance of organic photovoltaic cells (2009)

The power conversion efficiency of organic photovoltaic cells can be greatly enhanced by chemical doping to control the conductivity of the organic thin films. We demonstrate a nearly twofold improvement in the efficiency of planar heterojunction copper phthalocyanine/fullerene cells by n-doping the electron acceptor layer with decamethylcobaltocene in the vicinity of the fullerene/cathode interface. Doping improves the charge extraction efficiency and decreases the series resistance of the organic films, improving the current density and fill factor, respectively.
Benchtop fabrication of PDMS microstructures by an unconventional photolithographic method (2010)

Poly(dimethylsiloxane) (PDMS) microstructures have been widely used in bio-microelectromechanical systems (bio-MEMS) for various types of analytical, diagnostic and therapeutic applications. However, PDMS-based soft lithographic techniques still use conventional microfabrication processes to generate a master mold, which requires access to clean room facilities and costly equipment. With the increasing use of these systems in various fields, the development of benchtop systems for fabricating microdevices is emerging as an important challenge in their widespread use. Here we demonstrate a simple, low-cost and rapid method to fabricate PDMS microstructures by using micropatterned poly(ethylene glycol) diacrylate (PEGDA) master molds. In this method, PEGDA microstructures were patterned on a glass substrate by photolithography under ambient conditions and by using simple tools. The resulting PEGDA structures were subsequently used to generate PDMS microstructures by standard molding in a reproducible and repeatable manner. The thickness of the PEGDA microstructures was controllable from 15 to 300 µm by using commonly available spacer materials. We also demonstrate the use of this method to fabricate microfluidic channels capable of generating concentration gradients. In addition, we fabricated PEGDA microstructures by photolithography from the light generated from commonly available laminar cell culture hood. These data suggest that this approach could be beneficial for fabricating low-cost PDMS-based microdevices in resource limited settings.
Herpes simplex virus types 1 and 2 induce shutoff of host protein synthesis by different mechanisms in Friend erythroleukemia cells (1983)

Herpes simplex virus type 1 (HSV-1) and HSV-2 disrupt host protein synthesis after viral infection. We have treated both viral types with agents which prevent transcription of the viral genome and used these treated viruses to infect induced Friend erythroleukemia cells. By measuring the changes in globin synthesis after infection, we have determined whether expression of the viral genome precedes the shutoff of host protein synthesis or whether the inhibitor molecule enters the cells as part of the virion. HSV-2-induced shutoff of host protein synthesis was insensitive to the effects of shortwave (254-nm) UV light and actinomycin D. Both of the treatments inhibited HSV-1-induced host protein shutoff. Likewise, treatment of HSV-1 with the cross-linking agent 4,5',8-trimethylpsoralen and longwave (360-nm) UV light prevented HSV-1 from inhibiting cellular protein synthesis. Treatment of HSV-2 with 4,5',8-trimethylpsoralen did not affect the ability of the virus to interfere with host protein synthesis, except at the highest doses of longwave UV light. It was determined that the highest longwave UV dosage damaged the HSV-2 virion as well as cross-linking the viral DNA. The results suggest that HSV-2 uses a virion-associated component to inhibit host protein synthesis and that HSV-1 requires the expression of the viral genome to cause cellular protein synthesis shutoff.
Open-source testing tools for smart grid communication network (2013)

In any smart grid communication implementation, to determine the performance factor of the network, a testing of an end-to-end process flow is required. Therefore, an effective testing tool plays a crucial role in evaluating the performance of smart grid communications. Currently, there are a large number of tools and utilities that are available to study different performance parameters of a network. However, there have been a few studies investigating the testing tools for smart grid communication system. Moreover, most research works done previously focused on only one or two performance metrics such as delay and/or throughput. Plus, diverse communication technologies in smart grid creates major challenges for conventional testing software, for legacy testing programs are designed only for a single communication technology or standard in the network. Therefore, this paper discusses various open-source monitoring and testing tools and determines which set of tools is the most suitable for evaluating the performance of smart grid communication network.
The role of pulmonary intravascular macrophages in porcine reproductive and respiratory syndrome virus infection (2000)

The objective of this article is to summarize the current state of knowledge of the complex interaction of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine pulmonary intravascular macrophages (PIMs). PIMs play an important role in pulmonary surveillance, and in the past few years we have investigated their role in PRRSV infection. PRRSV antigens and nucleic acids have been demonstrated in PIMs both in vitro and in vivo. Examination of cultured PIMs infected with PRRSV revealed the accumulation of viral particles in the smooth-walled vesicles. PRRSV-infected PIMs in vitro yielded a virus titer similar to pulmonary alveolar macrophages. PRRSV infection induces either apoptosis or cell lysis of PIMs. The in vitro bactericidal activity of PRRSV-infected PIMs is significantly decreased. Phagocytic activity of PIMs, as measured by pulmonary copper clearance, is significantly decreased in PRRSV-infected pigs. This evidence supports the hypothesis that PRRSV-induced damage to PIMs results in increased susceptibility to bacteremic diseases. Recent studies with PRRSV and Streptococcus suis coinfection confirmed that PRRSV predisposes pigs to S. suis infection and bacteremia. These results could explain the increase in bacterial respiratory diseases and septicemias observed in PRRSV-infected pigs.
Cannabis Oil: chemical evaluation of an upcoming cannabis-based medicine (2013)

Concentrated cannabis extracts, also known as Cannabis oils because of their sticky and viscous appearance, are becoming increasingly popular among self-medicating patients as a claimed cure for cancer. In general, preparation methods for Cannabis oils are relatively simple and do not require particular instruments. The most well-known example of such a product is called ‘Simpson oil’. The purpose of the extraction, often followed by a solvent evaporation step, is to make cannabinoids and other beneficial components such as terpenes available in a highly concentrated form. Although various preparation methods have been recommended for Cannabis oils, so far no studies have reported on the chemical composition of such products. Recognizing the need for more information on quality and safety issues regarding Cannabis oils, an analytical study was performed to compare several generally used preparation methods on the basis of content of cannabinoids, terpenes, and residual solvent components. Solvents used include ethanol, naphtha, petroleum ether, and olive oil. The obtained results are not intended to support or deny the therapeutic properties of these products, but may be useful for better understanding the experiences of self-medicating patients through chemical analysis of this popular medicine.
Campus-Wide Computing : Early Results Using Legion At the University of Virginia (1997)

The Legion project at the University of Virginia is an architecture for designing and building system services that provide the illusion of a single virtual machine to users, a virtual machine that provides both improved response time via parallel execution and greater throughput. Legion targets workstation clusters and larger wide area assem blies of workstations, supercomputers, and parallel super computers. The authors have built a working Legion pro totype called the Campus-Wide Virtual Computer (CWVC). The CWVC extends an existing object-oriented parallel processing system by aggressively incorporating lessons learned in the last 20 years of heterogeneous distributed computing. In this paper, the authors describe the challenges that they overcame to realize a working CWVC and characterize the performance of a production biochemistry application.
La paz significa (2015)

Darle posibilidades a que el pais realice , en un ambiente propicio, una serie de reformas que la nueva realidad del pais exige. Se requiere ante todo modernizar el aparato del Estado lo que significa, democratizar la vidad del pais; lo primero son las reformas politicas, sin reformas politicas no se pueden dar las reformas sociales y economicas.
Thermally tunable optical aperture based on a segmented thin-film resonator (2014)

Apertures are basic elements which can be found in many optical systems. Since optical systems are continuously being miniaturized and integrated, there is a need for small and inexpensive apertures to control beam shape and light intensity. Current aperture concepts for the micrometer regime rely on moving MEMS lamella or controlling fluids by capillary or electrostatic forces. We demonstrate an aperture concept for single-wavelength operation based on thermal tuning of a segmented thin film resonator. Thermal tuning changes the optical thickness of the elastomer cavity. This allows for adjusting the intensity to any level between constructive and destructive interference in a specific aperture segment. In order to demonstrate aperture operation we simulate thermal, mechanical and optical properties using finite element method and transfer-matrix method. We confirm our simulation results by experimental beam shape measurements and spatially-resolved spectral transmission and light intensity measurements.
Red-Emitting (Sr,Ca)AlSiN<sub>3</sub>:Eu<sup>2+</sup>Phosphors Synthesized by Spark Plasma Sintering (2012)

Red-emitting (SrxCa1−x)AlSiN3:Eu2+ (SCASN:Eu2+) phosphors were successfully prepared by spark plasma sintering (SPS) at a relatively low temperature of 1700°C in vacuum (200 mtorr). The synthesized phosphors showed a broad absorption band from near UV to yellow region (300 ∼ 600 nm) and exhibited a single broad emission band centered at 626 ∼ 653 nm under the excitation of 450 nm. The effects of Sr occupancy and Eu doping concentration on the photoluminescence (PL) properties of SCASN:Eu2+ phosphors were examined. In addition, the temperature dependence of the PL intensity and the emission characteristics of white light-emitting InGaN-based β-sialon:Eu2+/SCASN:Eu2+ LED were compared with those of commercial YAG:Ce3+ phosphor and InGaN-based YAG:Ce3+ LED, respectively.
Multicell MIMO Communications Relying on Intelligent Reflecting Surfaces (2020)

Intelligent reflecting surfaces (IRSs) constitute a disruptive wireless communication technique capable of creating a controllable propagation environment. In this paper, we propose to invoke an IRS at the cell boundary of multiple cells to assist the downlink transmission to cell-edge users, whilst mitigating the inter-cell interference, which is a crucial issue in multicell communication systems. We aim for maximizing the weighted sum rate (WSR) of all users through jointly optimizing the active precoding matrices at the base stations (BSs) and the phase shifts at the IRS subject to each BS's power constraint and unit modulus constraint. Both the BSs and the users are equipped with multiple antennas, which enhances the spectral efficiency by exploiting the spatial multiplexing gain. Due to the non-convexity of the problem, we first reformulate it into an equivalent one, which is solved by using the block coordinate descent (BCD) algorithm, where the precoding matrices and phase shifts are alternately optimized. The optimal precoding matrices can be obtained in closed form, when fixing the phase shifts. A pair of efficient algorithms are proposed for solving the phase shift optimization problem, namely the Majorization-Minimization (MM) Algorithm and the Complex Circle Manifold (CCM) Method. Both algorithms are guaranteed to converge to at least locally optimal solutions. We also extend the proposed algorithms to the more general multiple-IRS and network MIMO scenarios. Finally, our simulation results confirm the advantages of introducing IRSs in enhancing the cell-edge user performance.
The Repeatability of Corneal Thickness Measures (2000)

The Orbscan system is the most repeatable technique for measuring corneal thickness but shows a significant bias toward greater corneal thickness measures than both ultrasound and optical pachymetry.
Solid phase phosphorus reagents. Conversion of alcohols to alkyl chlorides (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolid phase phosphorus reagents. Conversion of alcohols to alkyl chloridesSteven L. Regen and Dan P. LeeCite this: J. Org. Chem. 1975, 40, 11, 1669–1670Publication Date (Print):May 1, 1975Publication History Published online1 May 2002Published inissue 1 May 1975https://doi.org/10.1021/jo00899a042RIGHTS & PERMISSIONSArticle Views374Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (272 KB) Get e-Alerts Get e-Alerts
26 S Proteasome-mediated Production of an Authentic Major Histocompatibility Class I-restricted Epitope from an Intact Protein Substrate (1999)

Peptides displayed on the cell surface by major histocompatibility class I molecules (MHC class I) are generated by proteolytic processing of protein-antigens in the cytoplasm. Initially, antigens are degraded by the 26 S proteasome, most probably following ubiquitination. However, it is unclear whether this proteolysis results in the generation of MHC class I ligands or if further processing is required. To investigate the role of the 26 S proteasome in antigen presentation, we analyzed the processing of an intact antigen by purified 26 S proteasome. A recombinant ornithine decarboxylase was produced harboring the H-2K(b)-restricted peptide epitope, derived from ovalbumin SIINFEKL (termed ODC-ova). Utilizing recombinant antizyme to target the antigen to the 26 S proteasome, we found that proteolysis of ODC-ova by the 26 S proteasome resulted in the generation of the K(b)-ligand. Mass spectrometry analysis indicated that in addition to SIINFEKL, the N-terminally extended ligand, HSIINFEKL, was also generated. Production of SIINFEKL was linear with time and directly proportional to the rate of ODC-ova degradation. The overall yield of SIINFEKL was approximately 5% of the amount of ODC-ova degraded. The addition of PA28, the 20 S, or the 20 S-PA28 complex to the 26 S proteasome did not significantly affect the yield of the antigenic peptide. These findings demonstrate that the 26 S proteasome can efficiently digest an intact physiological substrate and generate an authentic MHC class I-restricted epitope.
Effects of the repartitioning agent ractopamine on the growth and body composition of rainbow trout, Oncorhynchus mykiss (Walbaum), fed three levels of dietary protein (1998)

Ractopamine is a synthetic catecholamine analogue which is known to affect the growth performance of both terrestrial and aquatic livestock species when it is incorporated into their feed. To investigate the effect of ractopamine on fish, rainbow trout, Oncorhynchus mykiss (Walbaum), (initial weight = 385.2 ± 1.4 g) were fed six iso-energetic (16.6 MJ kg−1) practical diets supplying two levels of ractopamine (0 and 10mgkg−1) at three levels of crude protein (CP; 25%, 35% and 45%) for 112 days. The mean weight gain (MWT), specific growth rate (SGR), condition factor (CF), viscerosomatic index (VSI), hepatosomatic index (HSI), muscle pigmentation intensity, and the proximate composition of eviscerated carcasses and viscera were measured at 28-day intervals up to day 112. There were no significant (P > 0.05) main effects of ractopamine on any of the responses measured. Significant (P & < 0.05) main effects of dietary protein were observed for MWT, SGR, CF, HSI, pigmentation, eviscerated-carcass ash, and visceral lipid and visceral ash content. Modest but significant (P < 0.05) interactions between ractopamine and protein were observed for HSI, pigmentation intensity and eviscerated carcass moisture content. The protein and lipid contents of both whole viscera and eviscerated carcasses were not affected (P > 0.05) by the inclusion of 10mgkg−1 ractopamine in the diet. These results indicate that ractopamine may not invoke the beneficial effects of enhancing protein accretion or reducing lipid content of rainbow trout when fed at 10mgkg−1 of diet at the protein levels tested.
Hexamethylmelamine and Low or Moderate Dose Cisplatin With or Without Pyridoxine for Treatment of Advanced Ovarian Carcinoma: A Study of the Eastern Cooperative Oncology Group (1992)

A total of 248 analyzable patients with Stages III-IV ovarian epithelial cancer (114 with and 134 without prior chemotherapy) were randomized to one of four cisplatin (DDP)-hexamethylmelamine (HMM) regimens. In each, HMM, 200 mg/m2 was given orally daily on days 8-21 of each 21-day cycle. DDP was given i.v. on Day 1 at a dose of 37.5 mg/m2 (regimens A and B) or 75 mg/m2 (regimens C and D). In addition, since pyridoxine administration has been reported to reduce the neurotoxicity of HMM, that agent was given at a dose of 300 mg/m2 orally on Days 1-21 in regimens B and D. Randomization was stratified for performance status (0-1, 2-3) and largest tumor diameter at entry (greater than 2- less than or equal to 10 cm, greater than 10 cm) for previously untreated patients, and for performance status and time from initial diagnosis to entry on study (less than or equal to 1 year, greater than 1 year) for previously treated patients. The overall response rate (PR + CR) was 54%, with 25% of patients achieving a complete response. The 61% response rate with the higher dose DDP regimens was significantly greater than the 47% response rate with the lower dose regimens (p = 0.031). Multivariate analysis identified higher DDP dose, age less than 60 years, no prior chemotherapy, small tumor bulk and favorable tumor grade as significant prognosticators for response. The overall median response duration was 8.3 months (range 1-70 months). Prior chemotherapy, pyridoxine administration, recent diagnosis, and large tumor size were identified by multivariate analysis as factors adversely affecting response duration. Patients treated with the higher dose DDP regimens had more severe nausea, vomiting, and neurotoxicity. This study demonstrates that the combination of DDP + HMM is an effective regimen for advanced ovarian carcinoma that yields response rates comparable to other more complex regimens, and that there is a dose-response relationship for DDP in ovarian cancer. Although pyridoxine administration significantly reduced neurotoxicity, its adverse effect on response duration suggests that the agent should not be administered with DDP or HMM. The mechanism by which pyridoxine may unfavorably affect response duration deserves further investigation.