gebhart/patrepeval huggingface dataset:
Hypertension and abnormal fat distribution but not insulin resistance in mice with P465L PPARγ (2004)

Peroxisome proliferator-activated receptor gamma (PPARgamma), the molecular target of a class of insulin sensitizers, regulates adipocyte differentiation and lipid metabolism. A dominant negative P467L mutation in the ligand-binding domain of PPARgamma in humans is associated with severe insulin resistance and hypertension. Homozygous mice with the equivalent P465L mutation die in utero. Heterozygous mice grow normally and have normal total adipose tissue weight. However, they have reduced interscapular brown adipose tissue and intra-abdominal fat mass, and increased extra-abdominal subcutaneous fat, compared with wild-type mice. They have normal plasma glucose levels and insulin sensitivity, and increased glucose tolerance. However, during high-fat feeding, their plasma insulin levels are mildly elevated in association with a significant increase in pancreatic islet mass. They are hypertensive, and expression of the angiotensinogen gene is increased in their subcutaneous adipose tissues. The effects of P465L on blood pressure, fat distribution, and insulin sensitivity are the same in both male and female mice regardless of diet and age. Thus the P465L mutation alone is sufficient to cause abnormal fat distribution and hypertension but not insulin resistance in mice. These results provide genetic evidence for a critical role for PPARgamma in blood pressure regulation that is not dependent on altered insulin sensitivity.
Higher expression of fab antibody fragments in a CHO cell line at reduced temperature (2003)

A chimeric Fab was expressed in Chinese hamster ovary cells under the control of the CMV promoter in a two-stage production process. Cells were first grown to 90% confluence at 37 degrees C in a proliferation phase, followed by a production phase at either 37 degrees C or 28 degrees C. Medium supplemented with serum and medium free from serum was tested in the production phase at both temperatures. Comparison of Fab expression revealed that reducing the temperature to 28 degrees C resulted in a 14-fold increase in product yield when cells were cultivated in serum-containing medium, and in a 38-fold increase in product yield when serum-free medium was applied.
A Case for Lehi’s Bondage in Arabia (1997)

Abstract The lengthy sojourn of Lehi’s family in the Arabian desert invites the almost inevitable question whether circumstances forced family members to live in the service of tribesmen either for protection or for food. In my view, enough clues exist in the Book of Mormon—they have to be assembled—to bring one to conclude that the family lived for a time in a servile condition, a situation that apparently entailed suffering and conflict.
Gas chromatographic method for measuring free lysine in the products of microbiological synthesis (1978)

The paper presents a gas chromatography technique for measuring free lysine. The technique allows lysine evaluation in the products and semi-products of microbiological synthesis for 50--80 min with an error of 4--5%.
HIVE-Hexagon: High-Performance, Parallelized Sequence Alignment for Next-Generation Sequencing Data Analysis (2014)

https://hive.biochemistry.gwu.edu/hive/
Quantitative Formation Permeability Evaluation from Stoneley Waves (1998)

Abstract Reservoir engineers need a continuous measurement of permeability to optimize well completion and produce the formation effectively. The borehole Stoneley wave has been shown to be sensitive to fluid mobility, the ratio of permeability and viscosity. The phenomenon is well described using the Biot theory, and models to account for the effect of the mudcake have been proposed. Existing techniques use either the Stoneley slowness or its attenuation at low frequency to estimate the fluid mobility with simplified Biot models. However, the results of these techniques usually show unexplained differences from core measured permeabilities, or with well test results. The objective of the methodology presented in this paper is to improve the reliability of the estimation and to take into account important factors that have been neglected. The evaluation technique described in this paper uses the Stoneley waveforms over a wide frequency range and inverts for fluid mobility applying the full Biot theory. The mudcake is modeled as an elastic membrane. The most important parameters in this evaluation are the mud slowness, mud attenuation, membrane stiffness and pore fluid compressibility. Practical techniques are proposed to obtain these parameters with sufficient accuracy from the recorded data. Stoneley waveform inversion provides a direct and continuous fluid mobility estimate near the wellbore (i.e., in the invaded zone). The intrinsic permeability of the rock can then be derived, provided that the various fluid components, their relative permeabilities and their viscosities are known. Finally the presence of irreducible water in the rock is also discussed as it can affect the estimation of mobility through the reduction of the effective porosity. The application of this methodology to actual data sets is the subject of an accompanying paper. The Stoneley wave provides a continuous estimation of the formation permeability along the well. Core measurements are not required although they can be used for verification. This technique will find applications in reservoir engineering optimization of well production through better placement of the perforated intervals. P. 389
[Correlations of CpG island methylator phenotype and OPCML gene methylation to carcinogenesis of hepatocellular carcinoma]. (2006)

CIMP exists in HCC, and may be a prognostic factor of HCC. Promoter methylation of OPCML gene may play an important role in hepatocarcinogenesis.
Prevention of pain on injection of propofol: systematic review and meta-analysis (2011)

The two most efficacious interventions to reduce pain on injection of propofol were use of the antecubital vein, or pretreatment using lidocaine in conjunction with venous occlusion when the hand vein was chosen. Under the assumption of independent efficacy a third practical alternative could be pretreatment of the hand vein with lidocaine or ketamine and use of a propofol emulsion containing medium and long chain triglycerides. Although not the most effective intervention on its own, a small dose of opioids before induction halved the risk of pain from the injection and thus can generally be recommended unless contraindicated.
Theoretical development of in situ optical particle separator: cross-type optical chromatography (2006)

An in situ separation system, cross-type optical chromatography, is developed theoretically, and an analytic solution of the retention distance is derived. Particle trajectories in the cross-type optical chromatography are calculated for various sizes and materials of the particles and for flow velocities. Further, cross-type optical chromatography assisted by a particle beam generation system is designed.
Microwave-Assisted Synthesis Utilizing Supported Reagents:  A Rapid and Efficient Acylation Procedure (2003)

[reaction: see text] The application of microwave heating to a polymer-assisted solution-phase (PASP) synthesis technique has been utilized to develop a rapid and efficient protocol for the solution-phase synthesis of amides from either amine or carboxylic acid cores.
Estimation of Brushing and Flowing Properties of Paints from Plasticity Data (1929)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEstimation of Brushing and Flowing Properties of Paints from Plasticity DataR. V. Williamson, G. D. Patterson, and James K. HuntCite this: Ind. Eng. Chem. 1929, 21, 11, 1111–1115Publication Date (Print):November 1, 1929Publication History Published online1 May 2002Published inissue 1 November 1929https://pubs.acs.org/doi/10.1021/ie50239a036https://doi.org/10.1021/ie50239a036research-articleACS PublicationsRequest reuse permissionsArticle Views31Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Molecular Imprinting of Biotin Derivatives and Its Application to Competitive Binding Assay Using Nonisotopic Labeled Ligands (2000)

Synthetic biotin-binding polymers were prepared by molecular imprinting. Methacrylic acid (MAA) was copolymerized with ethylene glycol dimethacrylate in the presence of biotin methyl ester (B-Me) in chloroform. Hydrogen-bonding-based complexation of B-Me with MAA generates the binding sites complementary to B-Me after extracting B-Me from the resulting copolymers. Data from NMR titration suggest a one-to-one prepolymerization complex formation of B-Me with MAA in chloroform. A possible complex structure was estimated by docking of the most stable conformers by intermolecular Monte Carlo conformational search under the assumption of a one-to-one association. The selectivity of the imprinted polymers was investigated and an imprinted polymer-based competitive binding assay for B-Me was demonstrated using biotin p-nitrophenyl ester as a nonisotopic-labeled ligand.
SERS-based detection of barcoded gold nanoparticle assemblies from within animal tissue:Non-invasive detection of barcoded gold nanoparticles (2013)

We have explored the potential of deep Raman spectroscopy, specifically surface‐enhanced spatially offset Raman spectroscopy (SESORS), for non‐invasive detection from within animal tissue, by employing SERS‐barcoded nanoparticle (NP) assemblies as the diagnostic agent. This concept has been experimentally verified in a clinically‐relevant backscattered Raman system with an excitation line of 785 nm under ex vivo conditions. We have shown that our SORS system, with a fixed offset of 2–3 mm, offered sensitive probing of injected 2‐quinolinethiol‐barcoded NP assemblies through animal tissue containing both protein and lipid. In comparison with that of non‐aggregated SERS‐barcoded gold NPs, we have demonstrated that the tailored SERS‐barcoded aggregated NP assemblies have significantly higher detection sensitivity. We report that these NP assemblies can be readily detected at depths of 7–8 mm from within animal proteinaceous tissue with high signal‐to‐noise ratio. In addition, they could also be detected from beneath 1–2 mm of animal tissue with high lipid content, which generally poses a challenge because of high absorption of lipids in the near‐infrared region. We have also shown that the signal intensity and signal‐to‐noise ratio at a particular depth is a function of the SERS tag concentration used and that our SORS system has a 2‐quinolinethiol detection limit of 10−6 M. Higher detection depths may possibly be obtained with optimization of the NP assemblies, along with improvements in the instrumentation. Such NP assemblies offer prospects for in vivo, non‐invasive detection of tumours along with scope for incorporation of drugs and their targeted and controlled release at tumour sites. These diagnostic agents combined with drug delivery systems could serve as a ‘theranostic agent’, an integration of diagnostics and therapeutics into a single platform.
The nematode parasite Meria coniospora Drechsler in pure culture and its classification (1985)

Volume: 22
Evaluation of Interleaved Source Coding over memoryless Binary Erasure Channel (2009)

Interleaved Source Coding (ISC) is one of the error resilient coding methods for predictive video coded frames transmitted over a single erasure channel. ISC has shown to significantly improve the overall quality of predictive coded video stream over a lossy channel. In this paper, ISC is evaluated over memoryless Binary Erasure Channel (ISC-BEC). Simulations have shown the superiority of ISC over memoryless BEC compared to the traditional method.
Microbe-Host Interactions: Structure and Role of Gram-Negative Bacterial Porins (2012)

Gram negative bacteria have evolved many mechanisms of attaching to and invading host epithelial and immune cells. In particular, many outer membrane proteins (OMPs) are involved in this initial interaction between the pathogen and their host. The outer membrane (OM) of Gram-negative bacteria performs the crucial role of providing an extra layer of protection to the organism without compromising the exchange of material required for sustaining life. The OM, therefore, represents a sophisticated macromolecular assembly, whose complexity has yet to be fully elucidated. This review will summarize the structural information available for porins, a class of OMP, and highlight their role in bacterial pathogenesis and their potential as therapeutic targets. The functional role of porins in microbe-host interactions during various bacterial infections has emerged only during the last few decades, and their interaction with a variety of host tissues for adhesion to and invasion of the cell and for evasion of host-defense mechanisms have placed bacterial porins at the forefront of research in bacterial pathogenesis. This review will discuss the role that porins play in activating immunological responses, in inducing signaling pathways and their influence on antibiotic resistance mechanisms that involve modifications of the properties of the OM lipid barrier.
Reorganization of endothelial cord‐like structures on basement membrane complex (Matrigel): Involvement of transforming growth factor β1 (1994)

The formation of capillary-like network structures by cultured vascular endothelial cells on reconstituted basement membrane matrix, Matrigel, models endothelial cell differentiation, the final step of angiogenesis (Kubota et al., 1988; Grant et al., 1989). When endothelial cells derived from bovine aorta and brain capillaries were plated on Matrigel, DNA synthesis was suppressed and a network of capillary-like structures rapidly formed in 8-12 h. With time, the network broke down, resulting in dense cellular cords radiating from multiple cellular clusters in 16-24 h. Finally, multicellular aggregates of cells were formed as the network underwent further retraction. Network regression was prevented when either dithiothreitol (DTT) or anti-TGF-beta 1 antibodies were added during the assay. The addition of exogenous TGF-beta 1 promoted the regression of endothelial cells into the clusters. This response to TGF-beta 1 was blocked by potent serine threonine protein kinase inhibitors, H-7 and HA100. TGF-beta 1 was released from polymerized Matrigel by incubation with Dulbecco's modified eagle's medium (DMEM) in the absence of cells. The Matrigel-conditioned DMEM inhibited endothelial DNA synthesis even in the presence of anti-TGF-beta 1 antibodies. These results suggest that TGF-beta 1 and possibly other soluble factors from Matrigel may be important for differentiation and remodeling of endothelial cells in a capillary network with possible implications for wound healing and development.
Naphtho[2,3-<i>b</i>:6,7-<i>b</i>′]dichalcogenophenes: Syntheses, Characterizations, and Chalcogene Atom Effects on Organic Field-Effect Transistor and Organic Photovoltaic Devices (2011)

New linear-shaped naphtho[2,3-b:6,7-b′]-difurans (NDFs) and -selenophenes (NDSs) were synthesized selectively from 3,7-dibromo-2,6-dihydroxynaphthalene and evaluated as organic semiconductors in comparison to corresponding naphtho[2,3-b:6,7-b′]dithiophenes (NDTs). Evaluation of the electronic structures of the parent compounds by means of electrochemical and optical measurements clearly indicated that NDT and NDS are quite similar to each other, whereas only NDF has a marked different electronic structure. Thin film devices, including organic field-effect transistors (OFETs) and bilayer photovoltaics (OPVs) with C60 or C70 as an acceptor layer, were fabricated with the diphenyl derivatives using vacuum deposition. The thin films were found to be nicely crystalline with the edge-on molecular orientation both on Si/SiO2 (for OFETs) and ITO substrates (for OPVs). The thin films acted as active semiconducting layer in OFETs with mobility higher than 0.1 cm2 V–1 s–1 and as a donor layer in OPVs with power conversion efficiencies of up to 2.0%, indicating that the present naphthodichalcogenophenes are potential core structures for the development of new organic semiconductors.
A Platform for Gaze-Contingent Virtual Environments (2000)

This paper describes hardware and software requirements for the development of a gaze-contingent virtual reality system which incorporates several cues to presence. The user&apos;s gaze direction, as well as head position and orientation, are tracked to allow dynamic level-of-detail changes for rendering. Users can see themselves, rather than representations thereof, within blue-screened virtual environments, and limited vestibular feedback is provided through a motion simulator. The aesthetic appearance of environments is driven by advanced graphical techniques (i.e., radiosity) motivated by the goal of photorealistic representation of natural scenes. Taken together, the components identified in this paper describe a platform suitable for development of a variety of &quot;smart&quot; virtual environments. Introduction The extent to which subjective telepresence enhances human performance remains undetermined, largely due to difficulties in delivering and measuring varying degrees of s...
Pharmacokinetic Investigation of Commercially Available Edible Marijuana Products in Humans: Potential Influence of Body Composition and Influence on Glucose Control (2021)

The purpose of the study was to describe and compare the pharmacokinetics of five commercial edible marijuana products, determine the influence of body composition on pharmacokinetics, and, in light of epidemiology suggesting marijuana may offer diabetes protection, explore the influence of edible marijuana on glucose tolerance. Seven regular users of marijuana self-administered five edible products in a randomized crossover design; each product contained 10 mg of delta-9-tetrahydrocannabinol (THC). Thirty minutes following marijuana ingestion, participants imbibed a 75 g glucose beverage. Time-to-peak plasma THC concentration ranged between 35 and 90 min; maximal plasma THC concentration (C<sub>max</sub>) ranged between 3.2 and 5.5 ng/mL. Differences between products in plasma THC concentration during the first 20-30 min were detected (<i>p</i> = 0.019). Relations were identified between body composition and pharmacokinetic parameters for some products; however, none of these body composition characteristics were consistently related to pharmacokinetics across all five of the products. Edible marijuana had no effect on oral glucose tolerance compared with a marijuana-free control (Matsuda Index; <i>p</i> > 0.395). Commercially available edible marijuana products evoke different plasma THC concentrations shortly after ingestion, but do not appear to influence acute glucose regulation. These data may allow recreational marijuana users to make informed decisions pertaining to rates of edible marijuana ingestion and avoid overdose.
Glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins (2000)

Metal-catalyzed oxidation results in loss of function and structural alteration of proteins. The oxidative process affects a variety of side amino acid groups, some of which are converted to carbonyl compounds. Spectrophotometric measurement of these moieties, after their reaction with 2,4-dinitrophenylhydrazine, is a simple, accurate technique that has been widely used to reveal increased levels of protein carbonyls in aging and disease. We have initiated studies aimed at elucidating the chemical nature of protein carbonyls. Methods based on gas chromatography/mass spectrometry with isotopic dilution were developed for the quantitation of glutamic and aminoadipic semialdehydes after their reduction to hydroxyaminovaleric and hydroxyaminocaproic acids. Analysis of model proteins oxidized in vitro by Cu2+/ascorbate revealed that these two compounds constitute the majority of protein carbonyls generated. Glutamic and aminoadipic semialdehydes were also detected in rat liver proteins, where they constitute approximately 60% of the total protein carbonyl value. Aminoadipic semialdehyde was also measured in protein extracts from HeLa cells, and its level increased as a consequence of oxidative stress to cell cultures. These results indicate that glutamic and aminoadipic semialdehydes are the main carbonyl products of metal-catalyzed oxidation of proteins, and that this reaction is a major route leading to the generation of protein carbonyls in biological samples.
Visualization of MG53-mediated Cell Membrane Repair Using <em>in vivo</em> and <em>in vitro</em> Systems (2011)

Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular understanding of the basic biology of tissue repair, as well as for potential translational applications in regenerative medicine. Here we detail the experimental protocols for exploring the in vivo function of MG53 in repair of muscle injury using treadmill exercise protocols on mouse models, for testing the ex vivo membrane repair capacity by measuring dye entry into isolated muscle fibers, and for monitoring the dynamic process of MG53-mediated vesicle trafficking and cell membrane repair in cultured cells using live cell confocal microscopy.
Iterative THP Transceiver Optimization for Multi-User MIMO Systems Based on Weighted Sum-Mse Minimization (2006)

We address the joint optimization of transmitter and receivers for a multi-user multiple-input multiple-output (MIMO) broadcast channel (BC) system under the assumption of perfect channel state information (CSI) at both transmitter and receivers. Tomlinson Harashima precoding (THP) is employed for interuser interference presubtraction and the mean square error (MSE) is minimized. Since the downlink problem is difficult to handle, we formulate an equivalent uplink problem by exploiting the duality between THP and decision feedback equalization (DFE). We present an iterative solution, which delivers suboptimum transmit and receive matrices as well as a suboptimum precoding order. The performance of the algorithm is studied theoretically and experimentally
Object-based analog VLSI vision circuits (2003)

Describes two analog VLSI chips that perform object-based computations. The first chip computes length of various contours in an image and the second chip performs figure-ground separation of a scene.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>