gebhart/patrepeval huggingface dataset:
Undesirable Charge-Enhancement of Isobaric Tagged Phosphopeptides Leads to Reduced Identification Efficiency (2010)

The study of cellular dynamics by proteomics using mass spectrometry requires a quantitation strategy that is robust, sensitive, and of sufficient resolution to deal with subtle changes in protein expression or post-translational modification. The major quantitation strategies are stable isotopic labeling of proteins and peptides for in vitro cell culture systems (stable isotope labeling using amino acids in cell culture, SILAC) or isobaric peptide labels such as isobaric tags for relative and absolute quantitation (iTRAQ) and tandem mass tags (TMT) for both in vitro and in vivo systems. These quantitation strategies have also been successfully applied to phosphoproteomics studies for the investigation of signal transduction pathways. Here we describe major drawbacks associated with isobaric labeling for the identification and quantitation of phosphopeptides using electrospray tandem mass spectrometry. Phosphopeptide derivatization with isobaric tags results in significantly greater charging in electrospray ionization. This reduces phosphopeptide identification efficiency with multistage activation and HCD MS/MS by more than 50% and may contribute to the discrepancy observed between identifications observed for large cell- or tissue-based data sets from labeled and nonlabeled peptide mixtures. Ammonia vapor sprayed perpendicular to the electrospray needle during ionization resulted in an overall decrease in the average charge states and a concomitant increase in phosphopeptide identifications.
Multiplexer-Based Architecture for High-Density, Low-Power Gate Arrays (2005)

This paper presents a novel architecture that provides higher density and lower power dissipation than conventional basecells. The layout of transistors in this small basecell allows the efficient construction of multiplexers with minimal use of programmable layers. The multiplexer can be used to create any 2 input and some 3 input functions in one basecell. Internal fanout, rather than typical output load, defines the size of driver and multiplexer transistors, which can be independently tailored for the desired speed/area/power target. This basecell, which is well suited for implementing datapath elements, has been used to create a 16 × 16-b multiplier operating at 50 MHz in 314 500 μm 2 in 0.6 μm technology
Amphiphilic block copolymers of vinyl ethers by living cationic polymerization. 3. Anionic macromolecular amphiphiles with pendant carboxylate anions (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmphiphilic block copolymers of vinyl ethers by living cationic polymerization. 3. Anionic macromolecular amphiphiles with pendant carboxylate anionsMasahiko Minoda, Mitsuo Sawamoto, and Toshinobu HigashimuraCite this: Macromolecules 1990, 23, 7, 1897–1901Publication Date (Print):April 1, 1990Publication History Published online1 May 2002Published inissue 1 April 1990https://pubs.acs.org/doi/10.1021/ma00209a001https://doi.org/10.1021/ma00209a001research-articleACS PublicationsRequest reuse permissionsArticle Views111Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Production scheduling systems using PROVISA (1994)

Describes the application of AT&T Istel's simulation-based finite-capacity scheduling system, PROVISA. The strategies that differentiate a finite-capacity scheduling system from a general-purpose simulation system are described. A brief description of PROVISA and how it fits into the manufacturing environment is presented. In addition, the steps required for building, implementing and verifying a PROVISA system are given.
Minocycline treatment in acute stroke (2007)

Patients with acute stroke had significantly better outcome with minocycline treatment compared with placebo. The findings suggest a potential benefit of minocycline in acute ischemic stroke.
Pulsed electromagnetic fields stimulate nerve regeneration in vitro and in vivo (1990)

The influence of non-invasive, low level, pulsed electromagnetic fields (PEMF) on regeneration was tested on in vitro and in vivo models. Cultures of dorsal root ganglia were exposed to 2 Hz PEMF, amplitude of 0.05 mTesla while rats after a 'crush' lesion of sciatic nerves were exposed to 2 Hz PEMF, amplitude of 0.3 mTesla. In in vitro experiments, relative to controls, cultures treated with PEMF exhibited a significant increase in neurite outgrowth with dense labeling of neurons and neurites on autoradiographs after incorporation of [3H]proline into new proteins. In vivo exposure of rats to PEMF for 3, 4 or 6 days after lesioning produced a 22% increase in the regeneration rate relative to controls with no effect on the initial delay period. When rats were exposed to PEMF before lesioning without further treatment, the same degree of stimulation of axonal sprouting was obtained. Reduction of the amplitude from 0.3 mTesla to 0.06 mTesla eliminated this pre-exposure response. Alterations in the distribution of new proteins synthesized 2 weeks after PEMF treatment provide additional evidence for its influence at the whole body and cellular levels.
Critical Intermediates Reveal New Biosynthetic Events in the Enigmatic Colibactin Pathway (2015)

Abstract Colibactin is a potent genotoxin that induces DNA double‐strand breaks; it is produced by Escherichia coli strains harboring a pks + island. However, the structure of this compound remains elusive. Here, using transformation‐associated recombination (TAR) cloning to perform heterologous expression, we took advantage of the significantly increased yield of colibactin pathway‐related compounds to determine and isolate a series of vital (pre)colibactin intermediates. The chemical structures of compounds 8 , 10 and 11 were identified by NMR and MS n analyses. The new 1 H ‐pyrrolo[3,4‐ c ]pyridine‐3,6(2 H ,5 H )‐dione‐ and thiazole‐containing compound 10 provides new insights regarding the biosynthetic pathway to (pre)colibactin and establishes foundations for future investigation of the intriguing (pre)colibactin structures and its modes of action.
Specific and selective site reactions in alkanoate derivatives. 1. Factors affecting .omega.-1 chlorinations by N-chloroamines (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpecific and selective site reactions in alkanoate derivatives. 1. Factors affecting .omega.-1 chlorinations by N-chloroaminesD. A. Konen, R. J. Maxwell, and L. S. SilbertCite this: J. Org. Chem. 1979, 44, 20, 3594–3596Publication Date (Print):September 1, 1979Publication History Published online1 May 2002Published inissue 1 September 1979https://pubs.acs.org/doi/10.1021/jo01334a037https://doi.org/10.1021/jo01334a037research-articleACS PublicationsRequest reuse permissionsArticle Views131Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Performance analysis of an expanding-search algorithm for coarse acquisition of DSSS signals (2002)

For coarse acquisition of direct sequence spread spectrum (DSSS) signals, a search of the possible code phases is required. In cases such as signal reacquisition, where a priori information is available, the serial search may be abandoned in favor of an expanding search. In this paper, an expanding search algorithm is presented and compared to the expanding-sweep algorithms which have been treated in the literature. Performance results for the serial-search and the expanding-search algorithms are given. A discussion of the optimality of the serial-search and the expanding-search algorithms is presented. Performance comparisons of the serial search and the expanding search are made for different a priori code phase probability density functions (pdfs) and the results are discussed. A technique for determining when the expanding search is an optimal search algorithm is developed and applied to the performance curves shown previously. The results indicate that the expanding search provides a quantifiable improvement over the serial search when the pdf is somewhat peaked. The results also indicate that in many cases the expanding search may be a more effective search algorithm than the more common expanding sweep.
<title>ColorSync 2.5: building a color platform</title> (1998)

Since ColorSync 1.0 was introduced in 1993, color management systems (CMS) are becoming a standard building block of modern operating systems. However, because CMS is an enabling technology, without other components in the system taking advantage of its capability, the end users may not experience the best results from this technology. This paper examines the approach that ColorSync takes to build a complete color platform which includes the following components: color management system with open framework, color savvy imaging model, easy ways to navigate and select color, device setup and calibration, scriptable interfaces, tightly integrated system. The paper points out the improvements over the previously released version.
Nonregenerative photonic dual bus with optical amplifiers (1993)

Nonregenerative photonic dual buses with passive optical access and optical amplification are investigated for transmission performance and potential network space for MAN/WAN. A two-wavelength system is proposed to improve the network capability, with one wavelength transmitted by the head of bus for distributing the clock and regulating the amplified spontaneous emission. The second wavelength is used by all nodes for network operations. It is shown that a multigigabit photonic dual bus with 100 nodes spanning hundreds of kilometers is possible. Major advantages of such networks are improved flexibility, reliability, uninterruptive maintenance, and graceful upgrade.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Mica Window Geiger Counter Tube for Measuring Soft Radiations (1943)

A mica window Geiger counter tube is described which combines the speed of a metal walled tube with a sensitivity to soft radiations comparable to that of the screen walled tubes. It consists of a NO2 treated metal cylinder covered at one end by a thin sheet of mica (0.02 mm) and at the other end by a glass bell with a central capillary. Through this capillary is sealed a piece of 8-mil tungsten wire with a drop of glass fused over the end. The tube is operated at a pressure of 10 cm of Hg with a mixture of 10 percent ethanol and 90 percent argon.
Phase I trial of temozolomide using an extended continuous oral schedule. (1998)

Temozolomide, a methylating imidazotetrazinone, has antitumor activity against gliomas, malignant melanoma, and mycosis fungoides and is presently administered as a 5-day oral schedule every 4 weeks. This Phase I study aimed to determine the maximum tolerated dose of temozolomide administered as a single oral daily dose for a continuous 6- or 7-week period, evaluate the plasma pharmacokinetics on this schedule, and compare total plasma exposure over 7 weeks with the conventional 5-day regimen. Twenty-four patients with varying tumor types (17 of 24 gliomas) received temozolomide. All had clinically evaluable, refractory disease; normal renal, hepatic, and bone marrow function; and WHO performance status < or = 2. Temozolomide was administered at 50 mg/m2/day, increasing by 25 mg/m2/day/cohort until at 100 mg/m2/day grade 4 myelotoxicity forced dose reductions to 85 mg/m2/day, then 75 mg/m2/day. At 75 mg/m2/day the regimen was extended to 7 weeks, allowing the future potential combination with irradiation for primary gliomas. Patient responses (standard Union International Contre Cancer criteria; for gliomas objective response) and toxicity were assessed. Temozolomide plasma pharmacokinetics were determined on day 1 and at the beginning of the final week of administration (n = 5). The most frequent toxicities were myelosuppression and grades 1 and 2 nausea and vomiting. Grade 4 leucopenia and thrombocytopenia occurred in one of four patients receiving 100 mg/m2/day temozolomide and in one of seven patients receiving 85 mg/m2/day. These hematological toxicities did not exceed grade 2 in 10 patients receiving 75 mg/m2/day temozolomide. One of 4 malignant melanoma patients and 7 of 17 glioma patients (41%) demonstrated tumor responses. The overall response rate for this prolonged schedule was 33% (objective response, 7 of 24 patients; partial response, 1 of 24 patients); also, 6 of 17 glioma patients maintained SD. Peak plasma temozolomide concentrations were obtained 30-90 min after oral administration. Elimination in plasma was best described by a monoexponential equation with an elimination half-life of 96 +/- 16 min. No plasma accumulation of temozolomide occurred. Toxicity was greatest in higher dose cohorts, with a resultant maximum tolerated dose of 85 mg/m2/day, whereas lower dose cohorts tolerated the schedule well. The area under the temozolomide plasma versus time curve was noncumulative between the first and last week of the schedule. Temozolomide administration of 75 mg/m2/day over a 7-week period permits a 2.1-fold greater drug exposure/4 weeks in comparison with the 5-day schedule of 200 mg/m2/day repeated every 28 days. The overall response rate was 33% (glioma patients, 41% and a further 25% SD). Temozolomide (75 mg/m2/day) for 7 weeks is the recommended starting dose for further assessment of this schedule.
Role of Oscillations in Membrane Potential, Cytoplasmic Ca2+, and Metabolism for Plasma Insulin Oscillations (2002)

A model for the relationship between ionic and metabolic oscillations and plasma insulin oscillations is presented. It is argued that the pancreatic beta-cell in vivo displays two intrinsic frequencies that are important for the regulation of plasma insulin oscillations. The rapid oscillatory activity (2--7 oscillations [osc] per minute), which is evident in both ionic and metabolic events, causes the required elevation in cytoplasmic Ca(2+) concentration ([Ca(2+)](i)) for the exocytosis of insulin granules. This activity is important for regulation of the amplitude of plasma insulin oscillations. The frequency of the rapid oscillatory ionic activities is regulated by glucose and allows the beta-cell to respond in an analogous way, with gradual changes in [Ca(2+)](i) and insulin release in response to the alterations in glucose concentration. The slower oscillatory activity (0.2--0.4 osc/min), which is evident in the metabolism of the beta-cell, has a frequency corresponding to the frequency observed in plasma insulin oscillations. The frequency is not affected by changes in the glucose concentration. This activity is suggested to generate energy in a pulsatile fashion, which sets the frequency of the plasma insulin oscillations. It is proposed that the slow oscillations in [Ca(2+)](i) observed in vitro are a manifestation of the metabolic oscillations and do not represent an in vivo phenomenon.
Magnetically Controlled Microwave Directional Coupler (1955)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation R. W. Damon; Magnetically Controlled Microwave Directional Coupler. Journal of Applied Physics 1 October 1955; 26 (10): 1281–1282. https://doi.org/10.1063/1.1721889 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioJournal of Applied Physics Search Advanced Search |Citation Search
Stimulation by nitric oxide of an NAD linkage to glyceraldehyde-3-phosphate dehydrogenase. (1993)

Nitric oxide-stimulated modification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by [adenylate-32P]NAD has been interpreted in recent reports as ADP-ribosylation. Incubations of GAPDH with the NO-releasing agent sodium nitroprusside (SNP) and NAD resulted, however, in essentially equal incorporation of radiolabel from the adenine, phosphate, and nicotinamide moieties to the extent of approximately 0.02 mol of NAD.mol of GAPDH-1. Modification of GAPDH by free adenosine 5'-diphosphoribose (ADP-ribose) was only 10% of that by NAD. Exposure of GAPDH modified by NAD in the presence of SNP to HgCl2, which acts at thiol linkages, released two products. Both contained nicotinamide and adenylate but did not cochromatograph with NAD. GAPDH activity was inhibited by SNP in a dose-dependent manner in the presence of NAD. When inhibition was 80%, with 1 mM SNP and 1 mM dithiothreitol, covalent modification with NAD was < 2%. This result is consistent with the conclusion that inhibition of GAPDH activity by SNP in the presence of NAD is due primarily to active-site nitrosylation, as reported by other workers, and is not due to the minor modification with NAD. These results demonstrate that NO-stimulated modification of GAPDH with NAD is not ADP-ribosylation as previously reported but rather is covalent binding of NAD through a NO-dependent thiol intermediate, possibly providing an example of an unexpected, altered reactivity of a nitrosylated protein.
Telomerase-Independent Telomere Length Maintenance in the Absence of Alternative Lengthening of Telomeres–Associated Promyelocytic Leukemia Bodies (2005)

Immortal tumor cells and cell lines employ a telomere maintenance mechanism that allows them to escape the normal limits on proliferative potential. In the absence of telomerase, telomere length may be maintained by an alternative lengthening of telomeres (ALT) mechanism. All human ALT cell lines described thus far have nuclear domains of unknown function, termed ALT-associated promyelocytic leukemia bodies (APB), containing promyelocytic leukemia protein, telomeric DNA and telomere binding proteins. Here we describe telomerase-negative human cells with telomeres that contain a substantial proportion of nontelomeric DNA sequences (like telomerase-null Saccharomyces cerevisiae survivor type I cells) and that are maintained in the absence of APBs. In other respects, they resemble typical ALT cell lines: the telomeres are highly heterogeneous in length (ranging from very short to very long) and undergo rapid changes in length. In addition, these cells are capable of copying a targeted DNA tag from one telomere into other telomeres. These data show that APBs are not always essential for ALT-mediated telomere maintenance.
Electrically controlled optical switch for multimode fiber applications (1980)

An electrically controlled optical switch based on polarization principles is described. It uses a liquid crystal twist cell that rotates polarization by 90 degrees , and it operates on an ac voltage that switches between 0.8 and 2.5 V rms. The switch has a loss of ~0.4 dB, neglecting reflections, in an unpolarized incoherent beam and a cross-talk ratio of ~-20 dB. It operates in collimated light, but it can be applied to multimode fibers using collimating lenses, which are expected to add <0.8 dB to the loss.
Sub-10 nm Self-Enclosed Self-Limited Nanofluidic Channel Arrays (2008)

We report a new method to fabricate self-enclosed optically transparent nanofluidic channel arrays with sub-10 nm channel width over large areas. Our method involves patterning nanoscale Si trenches using nanoimprint lithography (NIL), sealing the trenches into enclosed channels by ultrafast laser pulse melting and shrinking the channel sizes by self-limiting thermal oxidation. We demonstrate that 100 nm wide Si trenches can be sealed and shrunk to 9 nm wide and that lambda-phage DNA molecules can be effectively stretched by the channels.
Swirl-Inducing Ducts (2019)

This chapter examines the flow of swirling liquid in a duct. In many cases, circumferential velocity in the cross-section of a cylindrical duct is a remarkably linear function of radius up to the proximity of the duct wall. This is similar to the behaviour of a twisting solid shaft and the analogy leads to a solid body model for swirl flow in ducts. Helically profiled lobate duct walls provide a twisting torque, while wall friction in simple circular ducts causes swirl to decay. The liquid counterpart of the solid body is represented as a firstorder system in downstream distance because of the way torque is transmitted by duct walls rather than by shaft stiffness as in the solid case. The effect of the inertia of the rotating and twisting cylinder is unchanged from its solid counterpart, and damping is related to the viscosity of the liquid acting over the annulus between the rotating liquid cylinder and the duct wall. The shear stress in the liquid is shown to be linearly related to the intensity of the swirl. The generation of swirl is briefly described with reference to lobate designs, their development of shape and helix.
Subacute combined degeneration of the cord due to folate deficiency: response to methyl folate treatment. (1986)

Subacute combined degeneration of the cord is a rare complication of folate deficiency. Disturbance of methylation reactions in nervous tissue probably underlie subacute combined degeneration of the cord arising from folate as well as vitamin B12 deficiency. Methyl tetrahydrofolate is the form in which folic acid is transported into the CNS. Therefore methyl tetrahydrofolate treatment of the neurological and psychiatric manifestations of folate deficiency would seem to be theoretically advantageous. A case of subacute combined degeneration of the cord due to dietary folate deficiency and associated with an organic brain syndrome is reported. There was striking haematological, neurological and psychiatric response to methyl folate treatment.