gebhart/patrepeval huggingface dataset:
Active/de‐active state transition of the mitochondrial complex I as revealed by specific sulfhydryl group labeling (1999)

The sensitivities of NADH oxidase and/or NADH-ubiquinone reductase activities of submitochondrial particles and purified complex I towards N-ethylmaleimide (NEM) and other SH-reagents were studied. Only thermally de-activated preparations IA.D. Vinogradov (1998) Biochim. Biophys. Acta 1364, 169-185] were inhibited by SH-reagents whereas the redox-pulsed, activated enzyme was resistant to the inhibitors. The pH profile of the pseudo-first order inhibition rate suggested a pKa of about 10 for the de-activation-dependent, NEM-reactive sulfhydryl group. NADH-ubiquinone reductase of activated particles treated with an excess of NEM followed by removal of the inhibitor was still capable of slow reversible active/de-active transition. When active, NEM-treated particles were de-activated and further inhibited by N-fluorescein maleimide, specific incorporation of the fluorescence label into low molecular mass polypeptide was evident. Comparison of the specific fluorescence labeling of submitochondrial particles, crude and purified complex I showed that the active/de-active state-dependent SH-group is located in a 15 kDa polypeptide (most likely in the 15 kDa IP subunit of the iron-sulfur protein-containing fraction of complex I).
Isolation and structure of daphnetoxin, the poisonous principle of Daphne species (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsolation and structure of daphnetoxin, the poisonous principle of Daphne speciesGeorge H. Stout, Wayne J. Balkenhol, Mitchell Poling, and Gary L. HickernellCite this: J. Am. Chem. Soc. 1970, 92, 4, 1070–1071Publication Date (Print):February 1, 1970Publication History Published online1 May 2002Published inissue 1 February 1970https://pubs.acs.org/doi/10.1021/ja00707a058https://doi.org/10.1021/ja00707a058research-articleACS PublicationsRequest reuse permissionsArticle Views475Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Thermostable 35-Residue Subdomain within Villin Headpiece (1996)

The actin-bundling protein villin contains, at its extreme C terminus, a compact f-actin binding domain called "headpiece". This 76-amino acid domain from chicken is highly thermostable. Here, we show that the stable folded structure in headpiece is localized to a subdomain formed by the C-terminal 35 residues. The subdomain, denoted HP-35, is monomeric and retains high thermostability, with a Tm of 70( +/- 1) degree C at PH 7.0. There are no cysteine residues in HP-35 and its folding is not dependent on the binding of metals or other ligands. HP-35 is not a molten globule, but instead, has properties expected for a fully folded protein with a unique structure. In particular, the slowly exchanging amide protons in HP-35 have protection factors that are slightly larger than those predicted if exchange occurred only from globally unfolded molecules. NMR studies indicate that the headpiece subdomain contains three short alpha-helices, and that these same helices are present in the corresponding regions of intact headpiece. HP-35 is the smallest monomeric polypeptide characterized consisting of only naturally occurring amino acids that autonomously folds into a unique and thermostable structure without disulfide bonds or ligand binding.
A Randomized Trial Comparing the Safety, Adherence, and Pharmacodynamics Profiles of Two Doses of Sodium Bicarbonate in CKD: the BASE Pilot Trial (2019)

Both NaHCO<sub>3</sub> doses were well tolerated over 28 weeks with no significant difference in adverse events or hospitalization compared with placebo. The higher dose lowered urinary ammonium excretion and increased serum bicarbonate more than the lower dose but was associated with a greater increase in ACR. The higher 0.8 meq/kg of lean body wt per day dose of NaHCO<sub>3</sub> may be a reasonable choice for future trials.
KIR3DL1 and HLA-B Density and Binding Calibrate NK Education and Response to HIV (2016)

NK cells recognize self-HLA via killer Ig-like receptors (KIR). Homeostatic HLA expression signals for inhibition via KIR, and downregulation of HLA, a common consequence of viral infection, allows NK activation. Like HLA, KIR are highly polymorphic, and allele combinations of the most diverse receptor-ligand pair, KIR3DL1 and HLA-B, correspond to hierarchical HIV control. We used primary cells from healthy human donors to demonstrate how subtype combinations of KIR3DL1 and HLA-B calibrate NK education and their consequent capacity to eliminate HIV-infected cells. High-density KIR3DL1 and Bw4-80I partnerships endow NK cells with the greatest reactivity against HLA-negative targets; NK cells exhibiting the remaining KIR3DL1/HLA-Bw4 combinations demonstrate intermediate responsiveness; and Bw4-negative KIR3DL1(+) NK cells are poorly responsive. Cytotoxicity against HIV-infected autologous CD4(+) T cells strikingly correlated with reactivity to HLA-negative targets. These findings suggest that the programming of NK effector function results from defined features of receptor and ligand subtypes. KIR3DL1 and HLA-B subtypes exhibit an array of binding strengths. Like KIR3DL1, subtypes of HLA-Bw4 are expressed at distinct, predictable membrane densities. Combinatorial permutations of common receptor and ligand subtypes reveal binding strength, receptor density, and ligand density to be functionally important. These findings have immediate implications for prognosis in patients with HIV infection. Furthermore, they demonstrate how features of KIR and HLA modified by allelic variation calibrate NK cell reactive potential.
Virtual Network Reconfiguration in Optical Substrate Networks (2013)

This paper studies virtual network service provisioning over substrate optical networks and presents a novel reconfiguration scheme to improve efficiency. Simulation results show lower blocking and increased revenues as compared to existing strategies.
Selective inhibition of <i>Rhizopus</i> eumelanin biosynthesis by novel natural product scaffold-based designs caused significant inhibition of fungal pathogenesis (2020)

Melanin is a dark color pigment biosynthesized naturally in most living organisms. Fungal melanin is a major putative virulence factor of Mucorales fungi that allows intracellular persistence by inducing phagosome maturation arrest. Recently, it has been shown that the black pigments of Rhizopus delemar is of eumelanin type, that requires the involvement of tyrosinase (a copper-dependent enzyme) in its biosynthesis. Herein, we have developed a series of compounds (UOSC-1-14) to selectively target Rhizopus melanin and explored this mechanism therapeutically. The compounds were designed based on the scaffold of the natural product, cuminaldehyde, identified from plant sources and has been shown to develop non-selective inhibition of melanin production. While all synthesized compounds showed significant inhibition of Rhizopus melanin production and limited toxicity to mammalian cells, only four compounds (UOSC-1, 2, 13, and 14) were selected as promising candidates based on their selective inhibition to fungal melanin. The activity of compound UOSC-2 was comparable to the positive control kojic acid. The selected candidates showed significant inhibition of Rhizopus melanin but not human melanin by targeting the fungal tyrosinase, and with an IC50 that are 9 times lower than the reference standard, kojic acid. Furthermore, the produced white spores were phagocytized easily and cleared faster from the lungs of infected immunocompetent mice and from the human macrophages when compared with wild-type spores. Collectively, the results suggested that the newly designed derivatives, particularly UOSC-2 can serve as promising candidate to overcome persistence mechanisms of fungal melanin production and hence make them accessible to host defenses.
Speaking Clearly for the Hard of Hearing II (1986)

The first paper of this series (Picheny, Durlach, & Braida, 1985) presented evidence that there are substantial intelligibility differences for hearing-impaired listeners between nonsense sentences spoken in a conversational manner and spoken with the effort to produce clear speech. In this paper, we report the results of acoustic analyses performed on the conversational and clear speech. Among these results are the following. First, speaking rate decreases substantially in clear speech. This decrease is achieved both by inserting pauses between words and by lengthening the durations of individual speech sounds. Second, there are differences between the two speaking modes in the numbers and types of phonological phenomena observed. In conversational speech, vowels are modified or reduced, and word-final stop bursts are often not released. In clear speech, vowels are modified to a lesser extent, and stop bursts, as well as essentially all word-final consonants, are released. Third, the RMS intensities for obstruent sounds, particularly stop consonants, is greater in clear speech than in conversational speech. Finally, changes in the long-term spectrum are small. Thus, speaking clearly cannot be regarded as equivalent to the application of high-frequency emphasis.
Recognizing and reporting child abuse. (1996)

Studies have shown that up to two-thirds of child abuse injuries are inflicted on the head and neck region. Licensed dental providers are required by state law to report any cases of suspected child abuse or neglect. Therefore, it is important that the dental team learn the signs of child abuse and the simple mechanics of reporting suspected abuse. This article details some of these signs and how to document and report them.
Isogeny cordillera algorithm to obtain cryptographically good elliptic curves (2007)

The security of most elliptic curve cryptosystems is based on the intractability of the Elliptic Curve Discrete Logarithm Problem (ECDLP). Such a problem turns out to be computationally unfeasible when elliptic curves are suitably chosen. This paper provides an algorithm to obtain cryptographically good elliptic curves from a given one. The core of such a procedure lies on the usage of successive chains of isogenies, visiting different volcanoes of isogenies which are located in different l-cordilleras.
Chiral Induction in Originally Racemic Amino Acids <i>via</i> 5‐Acyl and 5‐Acyloxyaminooxazoles (1986)

Abstract A variety of chiral 2,4‐disubstituted‐5‐trifluoroacetamido‐,5‐carbobenzyloxyamino, and 5‐β,β,β,‐trichloroethoxycarbonylaminooxazoles have been prepared from diamide, dipeptide, or amide nitrile precursors. Conversion to their (poly)amide equivalents via a deacylation/hydration sequence is described. The extent of induction of chirality realized upon ring opening in each case is determined by HPLC.
Neuraxial (epidural and intrathecal) opioids for intractable pain (2012)

1. Neuraxial opioids are considered for use in patients who have resistant intractable pain that fails to respond to other treatment options or pain that responds to analgesia but for which the doses required result in unacceptable side-effects. 2. Neuraxial opiods can be considered for both chronic non-malignant pain and chronic cancer-related pain. 3. Effectiveness in chronic non-malignant pain and cancer pain is exerted through the use of either single-agent drugs (opioids) or a combination of drugs: opioids, local anaesthetics and other drugs such as clonodine and ziconotide. 4. Complications of long-term continuous infusion therapy are related to the insertion process (haematoma), the mechanical device (both pump and catheter) and the long-term effects of the drugs. 5. Patients will require ongoing ambulatory monitoring and supportive care.
Task Scheduling of Real-time Systems on Multi-Core Architectures (2009)

This paper proposes a hybrid scheduling method for real-time system on homogeneous multi-core architecture. To make the best of the available parallelism in these systems, first an application is partitioned into some parallel tasks as much as possible. Then the parallel tasks are dispatched to different cores, so as to execute in parallel. In each core, real-time applications can run concurrently with non-real-time applications. The scheduling architecture uses a two-level scheduling scheme. At the top level, a sporadic server is assigned to each scheduling policy. Each sporadic server is used to schedule the dispatched tasks according to its scheduling policy. At the bottom level, a rate-monotonic OS scheduler is adopted to schedule the top level sporadic servers.
Mask aligner process enhancement by spatial filtering (2011)

Mask Aligners are used in the Semiconductor Industry to transfer structures with moderate resolution requirements onto substrates. With the casting of the shadow a photochemical reactive resist is exposed. As diffraction appears at the mask structures the exposure wavelength and the proximity gap between mask and wafer influence the quality of the image in the resist. As both parameters are very often not changeable for processes there is a big need to find another way to improve the resist image. In this paper a new approach to enhance the exposure result will be presented. MO Exposure Optics, a novel illumination system for Mask Aligners, uses a combination of two microlens Köhler Integrators. MO Exposure Optics decouples the illumination system in a Mask Aligner from the lamp and ensures a uniform angular spectrum over the whole mask plane. Spatial filtering of the illumination light allows to reduce the diffraction effects at the mask structures and to improve the lithographic process in a Mask Aligner.
Differential Regulation of CCL22 Gene Expression in Murine Dendritic Cells and B Cells (2005)

The activated T cell-attracting CC chemokine CCL22 is expressed by stimulated B cells and mature dendritic cells (DC). We have cloned and sequenced the complete mouse gene, including 4 kb of the 5'-flanking promoter region, and detected two distinct sites for initiation of transcription by 5'-RACE. Reporter gene assays indicate that the promoter reflects the specificity of the endogenous gene. Within the proximal promoter region, we identified potential binding sites for NF-kappaB, Ikaros, and a putative GC box. All three regions bind proteins. The NF-kappaB site was shown to specifically bind NF-kappaB subunits p50 and p65 from nuclear extracts of LPS-stimulated B cells, B cell line A20/2J, TNF-alpha-stimulated bone marrow-derived DC, and DC line XS106. Furthermore, promoter activity was affected by targeted mutagenesis of the NF-kappaB site and transactivation with p50 and p65. The region harboring the putative Ikaros site contributes to promoter activity, but the binding protein does not belong to the Ikaros family. The GC box was shown to specifically bind Sp1 using extracts from LPS-stimulated B cells and A20/2J but not from DC and DC line XS106. Additionally, Sp1 transactivated the promoter in A20/2J but not in XS106 cells, and mutation of the Sp1 site diminished transactivation. Furthermore, binding of the protein complex at the GC box is required for NF-kappaB activity, and the spatial alignment of the binding sites is of critical importance for promoter activity. Thus, identical and distinct proteins contribute to expression of CCL22 in DC and B cells.
Autonomous development of a grounded object ontology by a learning robot (2007)

We describe how a physical robot can learn about objects from its own autonomous experience in the continuous world. The robot identifies statistical regularities that allow it to represent a physical object with a cluster of sensations that violate a static world model, track that cluster over time, extract percepts from that cluster, form concepts from similar percepts, and learn reliable actions that can be applied to objects. We present a formalism for representing the ontology for objects and actions, a learning algorithm, and the results of an evaluation with a physical robot.
Theoretical and Experimental Results on Active Vibration Dampers (1964)

The use of active feedback elements to produce damping of multiresonant structures is examined analytically, and the analysis is compared with experimental results obtained for a 48 in.-long uniform beam damped at its midpoint. The analysis method involves an adaption of the conventional Nichols-chart technique and appears to be quite reliable. By using commercially available components, a simultaneous reduction in resonant mobilities of resonances below 10 kc/sec is predicted and demonstrated. Reductions were proportional to the mobility values prior to active-damping applications and also to the gain in the feedback path. The limitations on the amount of damping possible are imposed by stability conditions for the feedback loop, which in turn depend primarily upon the transfer function of the forcing and pickup components of this loop. The stability analysis for particular components used in the beam experiment is presented.
Serum interferon gamma level predicts recurrence in hepatocellular carcinoma patients after curative treatments (2013)

Host immunity may have important role in the prognosis of hepatocellular carcinoma (HCC). The aim of this study was to evaluate the correlation between circulating immune regulators and clinical outcome in patients with HCC. Sixty-three HCC patients were prospectively enrolled. Serum levels of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), interferon-gamma (IFN-γ) and interferon gamma-inducible protein 10 (IP-10) were measured, as well as the prevalence of regulatory T cells (Treg), NK(+) T cells, invariant natural killer T cells (iNKT), programmed cell death-1 (PD-1)(+) CD8(+) T cells, T helper 17 cells (Th17), CD69(+) and CD45RO(+) T cells in peripheral blood mononuclear cells (PBMC). Correlation between these immune regulators and clinical outcome were analyzed. A low serum IFN-γ level (<50 pg/mL) was significantly associated tumor stage (BCLC stage B: 61.25% vs. stage A: 25%, p = 0.010) and tumor size (>5 cm: 53.8% vs. <5 cm: 25%, p = 0.047). Recurrence-free survival was evaluated in 48 patients receiving curative treatment of HCC. By multivariate analysis, BCLC stage [hazard ratio (HR) = 32.180, p < 0.001], tumor size (HR = 15.373, p = 0.005), AST (HR = 3.796, p = 0.011) and IFN-γ (HR = 0.354, p = 0.018) levels were independent factors associated with recurrence-free survival. In conclusion, serum IFN-γ level correlates with tumor stage and tumor size in HCC patients. Patients with lower baseline IFN-γ levels have a higher risk of tumor recurrence after curative treatment. IFN-γ may reflect host anti-tumor immunity and may be a potential marker of HCC recurrence after curative treatment.
<i>N</i>‐(pyrid‐3‐yl)thioureas and derivatives as acaricides. I. Synthesis and biological properties (1994)

Abstract In the course of optimization studies around diafenthiuron, the central aromatic nucleus linked directly to the thiourea unit was replaced by a pyridine moiety. A series of N ‐(pyrid‐3‐yl)thioureas, ‐isothioureas and ‐carbodiimides was synthesised and evaluated for acaricidal activity. The synthetic methodology used and the screening results against some spider mites ( Tetranychus spp. and Panonychus ssp.) are discussed.
Serotonin Metabolism Is Dysregulated in Cholangiocarcinoma, which Has Implications for Tumor Growth (2008)

Cholangiocarcinoma is a devastating cancer of biliary origin with limited treatment options. Symptoms are usually evident after blockage of the bile duct by the tumor, and at this late stage, they are relatively resistant to chemotherapy and radiation therapy. Therefore, it is imperative that alternative treatment options are explored. We present novel data indicating that the metabolism of serotonin is dysregulated in cholangiocarcinoma cell lines, compared with normal cholangiocytes, and tissue and bile from cholangiocarcinoma patients. Specifically, there was an increased expression of tryptophan hydroxylase 1 and a suppression of monoamine oxidase A expression (enzymes responsible for the synthesis and degradation of serotonin, respectively) in cholangiocarcinoma. This resulted in an increased secretion of serotonin from cholangiocarcinoma and increased serotonin in the bile from cholangiocarcinoma patients. Increased local serotonin release may have implications on cholangiocarcinoma cell growth. Serotonin administration increased cholangiocarcinoma cell growth in vitro, whereas inhibition of serotonin synthesis decreases tumor cell growth both in vitro and in vivo. The data presented here represent the first evidence that serotonin metabolism is dysregulated in cholangiocarcinoma and that modulation of serotonin synthesis may represent an alternative target for the development of therapeutic strategies.
The Novels (1968)

Hardy's first novel was written in the second half of 1867, after his return to Dorset from London. The first draft was completed in January 1868; the revised copy was finished by the early part of the following June. Initially described as 'A story with no plot, containing some original verses', it was never published. Portions of it survive, with modifications, in Hardy's other works; the manuscript was lost or destroyed.KeywordsSerial PublicationHappy EndingParadise LostRhine ValleyWeekly SerializationThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
A two-port digital discrete-time non-Foster circuit designed for negative capacitance (2015)

A digital discrete-time approach is presented for the design of two-port non-Foster circuits. The proposed digital approach offers the potential for effective implementation of complex non-Foster circuits in a wide variety of applications such as wideband metamaterials, broadband impedance matching, electrically small antennas, and artificial magnetic conductors. To illustrate the proposed digital design approach, a straightforward discrete-time implementation of a floating two-port negative capacitance is considered. Simulation results demonstrate the effectiveness of the implementation and design approach in producing -50 pF capacitance.