gebhart/patrepeval huggingface dataset:
Chi‐activated RecBCD enzyme possesses 5′→3′ nucleolytic activity, but RecBC enzyme does not: evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunit (1997)

We have shown that, despite the activation of a 5'-->3' exonuclease, recognition of chi by the RecBCD enzyme results in a net preservation of DNA. This new chi-activated nucleolytic action shows surprising variability in the exact location of its initial cleavage. We have demonstrated that purified RecBC enzyme is not an exact analogue of the chi-activated RecBCD enzyme, suggesting that the biochemical basis of chi-activation is not simply ejection of the RecD subunit.
Bile Salt-Stimulated Lipase Plays an Unexpected Role in Arthritis Development in Rodents (2012)

Our data strongly support BSSL as a key player in the inflammatory process, at least in rodents. It also suggests the possibility that BSSL-neutralizing agents could serve as a therapeutic model to reduce the inflammatory response in humans.
A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons (2014)

Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye. Our current device consists of a removable absorption filter interfaced with a CMOS imaging sensor and an external DG-4 lamp for excitation. Fura-2 loaded Lymnaea stagnalis neurons were stimulated with dual excitation wavelengths of 340 and 380 nm; our image sensor detected 510-nm emission. We show that our system is capable of detecting intracellular calcium changes in Fura-2 loaded neurons. Further, this sensor also enabled viewing of multiple neurons over a large surface area simultaneously, an option that is not readily available in conventional light microscopy.
Structure de Mg Si N2 et Mg Ge N2 (1970)

Les structures des deux nitrures MgSiN₂ et MgGeN₂ ont été étudiées à l'aide des diagrammes de poudre. Ces deux composés sont isotypes de BeSiN₂ et cristallisent dans le système orthorhombique. Le groupe spatial est Pna2₁ Le facteur R calculé en tenant compte de toutes les raies observées est 0,085 pour MgSiN₂ et 0,0645 pour MgGeN₂. Ces nitrures dérivent de la structure type wurtzite et forment une famille isostructurale avec BeSiN₂ et les composés oxygénés LiGaO₂ et β NaFeO₂.
Self-Assembling Supramolecular Nanostructures from a C60 Derivative: Nanorods and Vesicles (1999)

Simple modification of solution conditions provides facile access to supramolecular fullerene nanostructures. The fullerene derivative shown self-assembles to give nanorods or vesicles. The nanorods have diameters of 10-250 nm, depending on the counterion, and lengths greater than 70 µm. If ultrasonication is used, no nanorods form, but vesicles result having diameters of 10-70 nm and wall thicknesses of 3-6 nm.
Boğaz gargarası ile yapılan hızlı antijen testinin grup A beta-hemolitik streptokokları saptamadaki etkinliği (2008)

Objectives: The most important disadvantage of rapid antigen tests RAT is low sensitivity for the diagnosis of group A beta-hemolytic streptococci BHS . We determined the sensitivity, specificity, positive and negative predictive values of RAT in gargling samples. Patients and Methods: The study included 106 patients 53 females, 53 males, mean age 22±12 years; range 16-65 years with an initial diagnosis of tonsillopharyngitis. Swab samples were taken from the posterior pharyngeal wall and tonsil surface with a sterile cotton swab. Then, gargle samples were obtained by gargling for 10 seconds with sterile 0.9% NaCl to be examined by the RAT for group A BHS. Throat cultures were processed using standard microbiologic techniques. Results: Throat cultures were positive in 12 patients 11.3% and negative in 94 patients 88.6% . Rapid antigen test yielded five positive 4.7% and 101 negative 95.2% results. The sensitivity and specificity rates of RAT with throat gargling were 41.7% and 100%, with positive and negative predictive values of 100% and 93.1%, respectively. Conclusion: Despite satisfactory specificity, positive and negative predictive rates of RAT with throat gargling, its low sensitivity limits its use for the diagnosis of group A BHS.
Commercial Production and Management of Wild Bees-A New Entomological Industry (1970)

Journal Article Commercial Production and Management of Wild Bees-A New Entomological Industry Get access G. E. Bohart G. E. Bohart Entomology Research Division, Agricultural Research Service, Logan, Utah 84321 Search for other works by this author on: Oxford Academic Google Scholar Bulletin of the Entomological Society of America, Volume 16, Issue 1, 16 March 1970, Pages 8–9, https://doi.org/10.1093/besa/16.1.8 Published: 16 March 1970
Jahn-Teller Distortion in Dangling-Bond Linear Chains Fabricated on a Hydrogen-Terminated Si(100)-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mn>2</mml:mn><mml:mo>×</mml:mo><mml:mn>1</mml:mn></mml:math>Surface (1999)

Length-dependent charge redistribution in dangling-bond (DB) linear chains fabricated on a hydrogen-terminated $\mathrm{Si}(100)\ensuremath{-}(2\ifmmode\times\else\texttimes\fi{}1)$ surface is analyzed by using scanning tunneling microscopy and first-principles calculations. The second-layer Si atoms are displaced alternately to form pairs with charge redistribution, which is explained by the Jahn-Teller distortion in an artificial pseudomolecule. In a short even-numbered (DB) structure, an unpaired second-layer Si atom exists and behaves as a soliton accompanied by the flip-flop motion of the structure. We point out that the odd-even problem, the edge effect, and the finite length of the DB structures are indispensable to understand the relaxation in the structures.
Frações orgânicas e índice de manejo de carbono do solo em diferentes sistemas de produção orgânica (2011)

O efeito do manejo orgânico pode influenciar positivamente nas frações da matéria orgânica do solo.Este estudo objetivou avaliar as frações orgânicas do solo e o índice de manejo do carbono em áreas submetidas a manejo orgânico sob diferentes sistemas de uso do solo.As áreas selecionadas apresentavam os seguintes sistemas de uso do solo: preparo convencional (PC) com cultivo de milho, plantio direto (PD) com cultivo de berinjela, preparo reduzido (PR) com cultivo de figo, consórcio maracujá -Desmodium sp e um sistema agroflorestal (SAF).Uma área de floresta secundária foi tomada como referência da condição original do solo.As amostras de terra foram coletadas nas profundidades de 0-5 e 5-10 cm.Foi quantificado o carbono orgânico total (COT), o carbono orgânico particulado (COp) e o carbono orgânico associado aos minerais (COam).Em seguida calcularam-se os respectivos estoques (EstCOT, EstCOp e EstCOam) e o índice de manejo de carbono (IMC).Na profundidade de 0-5 cm observaram-se maiores teores de COT na área de figo (PR) seguido de berinjela (PD), sendo na profundidade de 5-10 cm verificado maiores diferenças entre as áreas avaliadas para este atributo.O EstCOp foi maior na área sob plantio direto (0-5 cm), sendo observado diferenças entre todas as áreas nesta profundidade.Esta área também apresentou o maior IMC.Os maiores valores de EstCOam foram observados na área de figo (PR), nas duas profundidades, sendo constatadas diferenças entre as demais áreas.Os resultados encontrados indicam que o COT foi mais eficiente para identificar mudanças provenientes do manejo adotado na profundidade de 5-10 cm, sendo este comportamento observado para o EstCOp em superfície (0-5 cm).O
Goniodomin A induces modulation of actomyosin ATPase activity mediated through conformational change of actin. (1993)

Goniodomin A, one of the polyether macrolides, isolated from the dinoflagellate Goniodoma pseudogoniaulax stimulated skeletal actomyosin ATPase activity 2-3 times, with a peak at 3 x 10(-8) to 3 x 10(-7) M. The effect of goniodomin A was dependent on the concentration of actin, but not of myosin. The actomyosin ATPase activity was increased by pretreatment of actin (but not of myosin) with goniodomin A. Goniodomin A induced a sustained and concentration-dependent increase in the fluorescence intensity (excitation wavelength, 277 nm; emission wavelength, 329 nm) of actin. The maximum response was obtained with concentrations of goniodomin A in the 10(-5) to 10(-4) M range in the presence of 5 microM F-actin. However, the ATPase activity and fluorescence intensity of myosin were not changed by goniodomin A at concentrations from 10(-8) to 10(-5) M. Interestingly, goniodomin A induced a remarkable but transient increase in the fluorescence intensity of actomyosin in a concentration-dependent manner, with a peak at 3 x 10(-7) M. This profile was quite similar to that found in the stimulation of the actomyosin ATPase activity induced by goniodomin A. To investigate further the effect of goniodomin A, actin was labeled with N-(1-pyrenyl)iodoacetamide. Goniodomin A at 10(-6) M had no effect on the fluorescence intensity of pyrenyl-actin (excitation wavelength, 365 nm; emission wavelength, 407 nm), but increasing concentrations of goniodomin A to 3 x 10(-6) M remarkably decreased its intensity. This effect was potentiated by heavy meromyosin. Actin molecules treated with goniodomin A were completely sedimented by mild centrifugation (for 15 min at 12,000 x g). Electron microscopic observations suggest that actin filaments associate with each other to form a gel in the presence of 3 x 10(-6) M goniodomin A. The conformational change of actin molecules, resulting from stoichiometric binding of goniodomin A to actin monomers in filaments, may modify the interaction between actin and myosin.
miR-141 as a regulator of the mitochondrial phosphate carrier (Slc25a3) in the type 1 diabetic heart (2012)

Dysfunctional mitochondria are central in the pathogenesis of diabetic cardiomyopathy. Mitochondrial proteomic alterations resulting from diabetes mellitus have been reported although the mechanisms driving changes in proteomic signatures are unknown. microRNAs (miRNAs) have been considered as potential regulators of proteins. The goal of this study was to determine whether miRNAs play a role in diabetes-induced mitochondrial proteomic alterations. Quanitative RT-PCR miRNA screening in diabetic mice, 5 wk following multiple low-dose streptozotocin treatment was associated with alteration in the expression of 29 miRNAs in the diabetic heart compared with control. Among those miRNAs upregulated in the diabetic heart was miR-141 (P < 0.002). miRNA target prediction analyses identified miR-141 as a potential regulator of the inner mitochondrial membrane phosphate transporter, solute carrier family 25 member 3 (Slc25a3), which provides inorganic phosphate to the mitochondrial matrix and is essential for ATP production. With the use of a luciferase reporter construct with a Slc25a3 3'-untranslated region (UTR) target sequence, overexpression of miR-141 downregulated luciferase activity levels confirming miR-141/Slc25a3 3'-UTR binding. miR-141 overexpression in HL-1 cells elicited a decrease in Slc25a3 protein content, ATP production and a decrease in ATP synthase activity, similar to the diabetic phenotype (P < 0.05, for both). Diabetic interfibrillar mitochondria (IFM) displayed decreased Slc25a3 protein content, which was inversely correlated with increased miR-141 expression. Further, diabetic IFM ATP synthase activity was also decreased (P < 0.05). Together these results indicate that miR-141 can regulate Slc25a3 protein expression in the diabetic heart. Further, diabetes-induced miRNA changes may influence mitochondrial proteomes and functional processes such as mitochondrial ATP production.
Digital image databasing (1995)

A previous two-part article by Simon Brown described the acquisition, editing, compression and initial storage of digital images taken in the clinical studio. Due to the higher-than-expected demand for this clinical service, additional factors had to be considered when dealing with large numbers of images. These included storage on writable compact disc (CD) drives and a system for retrieving images from these devices which was flexible enough to handle the requirements of different specialities and be usable by all medical illustration staff.
Neural Correlates of Natural Human Echolocation in Early and Late Blind Echolocation Experts (2011)

These findings suggest that processing of click-echoes recruits brain regions typically devoted to vision rather than audition in both early and late blind echolocation experts.
Bioavailability of resveratrol (2011)

This paper reviews our current understanding of the absorption, bioavailability, and metabolism of resveratrol, with an emphasis on humans. The oral absorption of resveratrol in humans is about 75% and is thought to occur mainly by transepithelial diffusion. Extensive metabolism in the intestine and liver results in an oral bioavailability considerably less than 1%. Dose escalation and repeated dose administration of resveratrol does not appear to alter this significantly. Metabolic studies, both in plasma and in urine, have revealed major metabolites to be glucuronides and sulfates of resveratrol. However, reduced dihydroresveratrol conjugates, in addition to highly polar unknown products, may account for as much as 50% of an oral resveratrol dose. Although major sites of metabolism include the intestine and liver (as expected), colonic bacterial metabolism may be more important than previously thought. Deconjugation enzymes such as β-glucuronidase and sulfatase, as well as specific tissue accumulation of resveratrol, may enhance resveratrol efficacy at target sites. Resveratrol analogs, such as methylated derivatives with improved bioavailability, may be important in future research.
Insulin-like growth factor I receptors in neuronal and glial cells. Characterization and biological effects in primary culture. (1987)

Primary cultures of neuronal and glial cells from 1-day-old neonatal rats contain high affinity receptors for insulin-like growth factor I (IGF-I). The IC50 for displacement of 125I-IGF-I binding by unlabeled IGF-I was 3 nM for neuronal cells and 4 nM for glial cells. Unlabeled insulin was 20-50 times less potent. Apparent molecular mass of the alpha subunits of the IGF-I receptor was 125 kDa in neuronal and 135 kDa in glial cells. IGF-I induced autophosphorylation of the IGF-I receptor beta subunit in lectin-purified membrane preparations in a dose-dependent manner. The major phosphoamino acid of the beta subunit in both cell types was tyrosine in the IGF-I-stimulated state and serine in the basal state. Apparent molecular mass of the beta subunits of the IGF-I receptors was 91 kDa for neuronal and 95 kDa for glial cells. Tyrosine kinase activity of the IGF-I receptors was demonstrated by IGF-I-induced phosphorylation of the exogenous substrate poly(Glu, Tyr) 4:1 in both cell types. IGF-I had no effect on 2-deoxyglucose uptake in neuronal cells. In contrast, in glial cells, IGF-I stimulated 2-deoxyglucose uptake at very high doses, presumably acting via the insulin receptor. The effect of IGF-I as a neurotrophic growth factor in both neuronal and glial cells was demonstrated by its stimulation of [3H]thymidine incorporation. These findings suggest the IGF-I is an important growth factor in nervous tissue-derived cells.
Retroviral transfer of genes into canine hemopoietic progenitor cells in culture: a model for human gene therapy. (1986)

Amphotropic retroviral vectors containing either a mutant dihydrofolate reductase gene (DHFR) or the bacterial neomycin phosphotransferase gene (neo) were used to infect canine hemopoietic cells. We report successful transfer and expression of the DHFR and neo genes in canine hemopoietic progenitor cells (colony-forming units, granulocyte/macrophage) as measured by the ability of the viruses to confer resistance to either methotrexate or the aminoglycoside G418, respectively. Transfer was achieved in the absence of helper virus by using retrovirus packaging cell lines. Successful transfer of these genes into canine hemopoietic progenitor cells in vitro indicates the feasibility of gene transfer into canine marrow for autologous reconstitution. Studies of transfer of new genetic information into a large, outbred animal such as the dog will provide a preclinical model for future gene therapy in humans.
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement (2001)

The abundant chromosome abnormalities in most carcinomas are probably a reflection of genomic instability present in the tumor, so the pattern and variability of chromosome abnormalities will reflect the mechanism of instability combined with the effects of selection. Chromosome rearrangement was investigated in 17 colorectal carcinoma-derived cell lines. Comparative genomic hybridization showed that the chromosome changes were representative of those found in primary tumors. Spectral karyotyping (SKY) showed that translocations were very varied and mostly unbalanced, with no translocation occurring in more than three lines. At least three karyotype patterns could be distinguished. Some lines had few chromosome abnormalities: they all showed microsatellite instability, the replication error (RER)+ phenotype. Most lines had many chromosome abnormalities: at least seven showed a surprisingly consistent pattern, characterized by multiple unbalanced translocations and intermetaphase variation, with chromosome numbers around triploid, 6-16 structural aberrations, and similarities in gains and losses. Almost all of these were RER-, but one, LS411, was RER+. The line HCA7 showed a novel pattern, suggesting a third kind of genomic instability: multiple reciprocal translocations, with little numerical change or variability. This line was also RER+. The coexistence in one tumor of two kinds of genomic instability is to be expected if the underlying defects are selected for in tumor evolution.
Development of micro-active forceps for future microsurgery (2001)

A new eye microsurgical system is proposed. The final goal of our research project has been not only to improve surgical instruments, but also to establish a total surgical system applicable to today's most difficult microsurgery, within the eye. The new prototype micro-forceps, which have a joint that enlarges the surgical area and a built-in thin optical fibrescope to increase the dexterity of retinal microsurgery, was designed as the first step in a long-term research plan. The micro-active forceps is equipped with a thin optical fibre inside the stem, giving a lateral view of the retina and a drastic improvement in fine operations. Moreover, this new tool, with an active joint and an end-effector, could find application in other microsurgical fields, such as in the brain, ear, nose and throat.
An Execution Service for Grid Computing (2004)

This paper describes the design and implementation of the IPG Execution Service that reliably executes complex jobs on a computational grid. Our Execution Service is part of the IPG service architecture whose goal is to support location-independent computing. In such an environment, once n user ports an npplicntion to one or more hardware/software platfrms, the user can describe this environment to the grid the grid can locate instances of this platfrm, configure the platfrm as required for the application, and then execute the application. Our Execution Service runs jobs that set up such environments for applications and executes them. These jobs consist of a set of tasks for executing applications and managing data. The tasks have user-defined starting conditions that allow users to specih complex dependencies including task to execute when tasks fail, afiequent occurrence in a large distributed system, or are cancelled. The execution task provided by our service also configures the application environment exactly as specified by the user and captures the exit code of the application, features that many grid execution services do not support due to dflculties interfacing to local scheduling systems.
Inventing for Dummies (2004)

Foreword.Introduction.Part I: Making Your Idea Yours.Chapter 1: The Innovation Process.Chapter 2: Patent Basics.Chapter 3: Conducting a Patent Search.Chapter 4: Applying For and Receiving Your Patent.Chapter 5: Maintaining and Defending Your Patent.Part II: Securing Other Intellectual Property.Chapter 6: Trademarks and Trade Secrets.Chapter 7: Stopping Copycats with a Copyright.Chapter 8: Mum's the Word: Keeping it Confidential.Part III: Developing Your Idea.Chapter 9: Prototyping: Making It Work.Chapter 10: Hiring Helpers and Working with Work-for-Hire Agreements.Chapter 11: Evaluating Your Invention's Potential.Chapter 12: Looking at the Production Process.Part IV: Commercializing Your Invention.Chapter 13: Developing a Business Plan.Chapter 14: Finding Funding.Chapter 15: Keeping Control with Your Own Business.Chapter 16: Partnering and Manufacturing Arrangements.Chapter 17: Preparing to Take Your Invention to Market.Chapter 18: Marketing Your Product.Chapter 19: Advertising Your Product.Chapter 20: Licensing Your Product.Chapter 21: Negotiating a License.Part V: The Part of Tens.Chapter 22: Ten Key Contacts.Chapter 23: Ten Inventions (and Inventors) That Changed the World.Chapter 24: Ten Inventors to Emulate.Part VI: Appendixes.Appendix A: Sample Agreements.Appendix B: Online Resources.Index.
Interventional radiologic procedures with CT guidance in cancer pain management. (1996)

Reduction of pain without systematic side effects can be achieved in advanced stages of cancer with precise percutaneous techniques guided with computed tomography (CT). CT guidance allows exact needle positioning, reducing complications and improving the results. Regional analgesia with neurolytic block (neurolysis) is achieved by injection of alcohol or phenol and involves intentional destruction of a nerve or nerves to interrupt nociceptive pathways for weeks or months. Percutaneous alcoholization of bone metastasis is indicated in patients with painful, severe, osteolytic bone metastasis if conventional anticancer therapy is ineffective and high doses of opiates are necessary to control pain and when rapid pain relief is necessary. Bone packing with acrylic glue (methyl methacrylate) is used to prevent pathologic fractures and pain in patients with vertebral body tumors and acetabular metastasis. With these techniques, the radiologist is able to play an active role in pain management and in improving the quality of life of patients with malignancies.
[Quantitative study of the granulopexy activity of the reticuloendothelial system by intravenous injection of India ink in different animal species. I. Method of quantitative study of the granulopexy activity of the reticuloendothelial system by intravenous injection of carbon particles of known dimensions]. (1951)

Abstract : The report describes a method which permits the quantitative evaluation of the granulopectic activity of the reticuloendothelial system in contact with the blood in various animal species. This method consists of introducing, intravenously, a homogenous and standardized suspension of carbon, in order to study the kinetics of its disappearance from the blood and its accumulation in the various organs by means of quantitative measurements. In the rat, during the injection of carbon doses of up to 16 mg per 100 g, the disappearance of the carbon from the blood takes place as a direct function of the injected dose. For these doses only the hepatosplenic territories of the reticuloendothelial system and of the hemopoietic tissue participate in the fixation of carbon granules. In the mouse the rate of disappearance of carbon from the blood is directly proportional to the amount injected, up to the dose of 32 mg per 100 g. The phenomenon of the acceleration of blood cleaning appears in this animal species only at doses that are essentially the double of those used in the rat. However, even at these high doses the phenomenon of acceleration of blood cleansing and the degree of pulmonary fixation of the carbon are more attenuated and irregular in the mouse than in the rat.