gebhart/patrepeval huggingface dataset:
Charge determination of product ions formed from collision-induced dissociation of multiply protonated molecules via ion/molecule reactions (1991)

The use of ion/molecule reactions involving multiply protonated ions derived from electrospray for the determination of the charges of product ions formed from collision-induced dissociation is described. The experiments are carried out with a quadrupole ion trap capable of multiple stages of mass spectrometry. The approach is illustrated with proton transfer from a product ion from quadruply protonated melittin, and from a product ion from the (M + 20H)20+ ion from horse myoglobin, to 1,6-diaminohexane. The major product ion from quadruply protonated bovine insulin is used to illustrate the use of a clustering reaction with 1,6-diaminohexane. The ion trap is shown to be a particularly useful tool for employing both collisional activation and low-energy ion/molecule reactions in the same experiment to determine product ion charge.
A novel Au-nanoparticle biosensor for the rapid and simple detection of PSA using a sequence-specific peptide cleavage reaction (2013)

PSA (prostate-specific antigen) is one of the most widely used proteins for the diagnosis of breast and prostate cancer. Of note, PSA displays enzymatic activity for the specific peptide sequence HSSKLQ, which it recognizes and cleaves. In this study, we developed a site-specific enzymatic-cleavage-reaction-based biosensor for the detection of PSA using fluorescein isothiocyanate (FITC)/peptide-conjugated gold (Au) nanoparticle complexes (FPANs). The FPANs do not initially fluoresce in the spectral region associated with the fluorophore, due to the quenching effect of the Au nanoparticles. When PSA was added to a solution containing the FPANs, PSA recognized and cleaved the specific sequence of the peptides attached to the Au nanoparticles. As a result, FITCs were separated from the Au nanoparticles and emitted strong fluorescence in their spectral region. Using this detection method, PSA was successfully detected as a function of concentration (10 pM-100 nM). This approach is superior to the immunoassay with respect to the performance of sensor, which is very rapid, simple, and one-step method for the detection of PSA and other protein markers can be measured for the early detection of several diseases.
Effect of heparin on vascular smooth muscle cells. II. Specific protein synthesis (1985)

Abstract Heparin suppresses the proliferation of vascular smooth muscle cells both in vivo and in vitro. The mechanism of action of the antiproliferative activity of heparin is not known. We have detected differences in the synthesis of specific proteins when vascular smooth muscle cells are exposed to heparin and report here that many characteristics of these protein alterations parallel the properties of the antiproliferative activity. The induction into the culture medium of a pair of proteins of approximately 35,000 dalton mw in heparintreated smooth muscle cell cultures and the antiproliferative effect of heparin share the following characteristics: 1) the effect is reversible, 2) the effect is specific for smooth muscle cells, 3) anticoagulant and non‐anticoagulant heparin are equally effective, 4) the effect is lost with time in culture and, 5) thermore, heparin causes a transient suppression of a 48,000 dalton substrateattached protein, whereas chondroitin sulfate A and C and dermatan sulfate had much less effect. Dextran sulfate was almost as effective as heparin in suppressing the synthesis of the substrate‐attached protein. These proteins appear to be noncollagenous and the induced synthesis of the 35,000 dalton proteins is inhibited by actinomycin D. Although a direct relationship between these specific protein changes and the antiproliferative effect of heparin has not been proven, these protein alterations may play a crucial role in the effect of heparin on smooth muscle cell growth.
Orbital Overlap and Chemical Bonding (2006)

The chemical bonds in the diatomic molecules Li(2)-F(2) and Na(2)-Cl(2) at different bond lengths have been analyzed by the energy decomposition analysis (EDA) method using DFT calculations at the BP86/TZ2P level. The interatomic interactions are discussed in terms of quasiclassical electrostatic interactions DeltaE(elstat), Pauli repulsion DeltaE(Pauli) and attractive orbital interactions DeltaE(orb). The energy terms are compared with the orbital overlaps at different interatomic distances. The quasiclassical electrostatic interactions between two electrons occupying 1s, 2s, 2p(sigma), and 2p(pi) orbitals have been calculated and the results are analyzed and discussed. It is shown that the equilibrium distances of the covalent bonds are not determined by the maximum overlap of the sigma valence orbitals, which nearly always has its largest value at clearly shorter distances than the equilibrium bond length. The crucial interaction that prevents shorter bonds is not the loss of attractive interactions, but a sharp increase in the Pauli repulsion between electrons in valence orbitals. The attractive interactions of DeltaE(orb) and the repulsive interactions of DeltaE(Pauli) are both determined by the orbital overlap. The net effect of the two terms depends on the occupation of the valence orbitals, but the onset of attractive orbital interactions occurs at longer distances than Pauli repulsion, because overlap of occupied orbitals with vacant orbitals starts earlier than overlap between occupied orbitals. The contribution of DeltaE(elstat) in most nonpolar covalent bonds is strongly attractive. This comes from the deviation of quasiclassical electron-electron repulsion and nuclear-electron attraction from Coulomb's law for point charges. The actual strength of DeltaE(elstat) depends on the size and shape of the occupied valence orbitals. The attractive electrostatic contributions in the diatomic molecules Li(2)-F(2) come from the s and p(sigma) electrons, while the p(pi) electrons do not compensate for nuclear-nuclear repulsion. It is the interplay of the three terms DeltaE(orb), DeltaE(Pauli), and DeltaE(elstat) that determines the bond energies and equilibrium distances of covalently bonded molecules. Molecules like N(2) and O(2), which are usually considered as covalently bonded, would not be bonded without the quasiclassical attraction DeltaE(elstat).
Analytical Aspects of Chemical and Biological Assay in the U. S. Army CBR Agency (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalytical Aspects of Chemical and Biological Assay in the U. S. Army CBR AgencySamuel SassCite this: Anal. Chem. 1963, 35, 6, 25A–37APublication Date (Print):May 1, 1963Publication History Published online18 May 2012Published inissue 1 May 1963https://pubs.acs.org/doi/10.1021/ac60199a719https://doi.org/10.1021/ac60199a719research-articleACS PublicationsRequest reuse permissionsArticle Views27Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optical activity of insulin. I. Nature of the circular dichroism bands (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOptical activity of insulin. I. Nature of the circular dichroism bandsSerge N. Timasheff, Murray J. Ettinger, and Mrs. G. D. StrycharzCite this: Biochemistry 1971, 10, 5, 824–831Publication Date (Print):March 1, 1971Publication History Published online1 May 2002Published inissue 1 March 1971https://pubs.acs.org/doi/10.1021/bi00781a015https://doi.org/10.1021/bi00781a015research-articleACS PublicationsRequest reuse permissionsArticle Views483Altmetric-Citations57LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Emerging oral drugs for multiple sclerosis (2008)

It is unknown whether these oral drugs could be used as first-line treatment for MS; this will depend mostly on their safety profile. Alternatively, these drugs could be used as add-on treatment for failed first-line therapy, or as an effective induction agent.
β-amyloid induction of gelatinase B secretion in cultured microglia (1996)

Since administration of anti-inflammatory drugs has been reported to possibly slow the progression of Alzheimer's disease, an effort was made to define effector functions induced by beta-amyloid (A beta) which may be inhibited by these drugs. Rat microglia in culture were treated with various A beta fragments and gelatinase (GLase) activity was determined using gelatin-substrate zymography; the ability of indomethacin (INDO) and dexamethasone (DEX) to inhibit A beta-stimulated GLase, specifically matrix metalloproteinase-9 (MMP-9), activity was also evaluated. A beta (1-38) and A beta (1-40) were effective in stimulating MMP-9 activity in a dose-dependent fashion. Co-treatment with INDO (50 microM) or DEX (100 nM) resulted in a 54% and 66% inhibition, respectively, of A beta (1-40)-stimulated MMP-9 production in microglia.
Identification of Tasks Performed by Stroke Patients Using a Mobility Assistive Device (2006)

Many stroke patients are prescribed canes or other mobility assistive devices. Once taken home, these mobility assistive devices are often abandoned or misused. A means for assessing the use of the cane in the home and community settings is required to assist clinicians in the prescription of these devices. In this study, we propose the use of wearable sensors to identify tasks performed by stroke patients with a mobility assistive device. Subjects performed ten tasks with a three-axis accelerometer attached to their ankle and a neural network was trained to identify the task being performed. Results from 15 stroke patients indicated that these motor tasks can be reliably identified with a median sensitivity of 90 % at a median specificity of 95%. These results indicate that it is possible to use a single module with a three-axis accelerometer attached to the ankle to reliably identify motor tasks associated with the use of a cane. Therefore, we envision that the methodology presented in this paper could be used to evaluate the use of a cane in the context of the task being performed.
Combining Phosphate and Bacteria Removal on Chemically Active Filter Membranes Allows Prolonged Storage of Drinking Water (2013)

A chemically active filtration membrane with incorporated lanthanum oxide nanoparticles enables the removal of bacteria and phosphate at the same time and thus provides a simple device for preparation of drinking water and subsequent safe storage without using any kind of disinfectants.
Novel Fluorinated Polymer Materials Based on 2,3,5,6-Tetrafluoro-4-methoxystyrene (2003)

2,3,5,6-Tetrafluoro-4-methoxystyrene (TFMS) has been polymerized in bulk and in xylene solution by Atom Transfer Radical Polymerization (ATRP) in a conventional protocol at 110 °C. Relatively good control has been achieved with number-average molecular mass (Mn) up to 17,000 and corresponding polydispersity index (PDI) generally below 1.3. The ATRP of TFMS is the fastest observed for any substituted styrene in bulk or solution in organic solvents. The isolated poly(tetrafluoromethoxystyrene)s (PTFMS)s have been employed as macroinitiators for ATRP of 2,3,4,5,6-pentafluorostyrene (FS) and styrene (St) resulting in block copolymers with controlled characteristics. TFMS homo- and block copolymers with PS have better thermal stability than PS. The solubility of the PTFMS containing polymers is lower than that of PS. Furthermore, PTFMS has been demethylated and the resulting hydroxyl sites alkylated with different azobenzene side chains. The azobenzene derivatized polymer has additionally been copolymerized with St. Both homo- and block copolymers with azobenzene side chains form materials exhibiting liquid crystallinity.
Acute hormonal response to sublingual androstenediol intake in young men (2002)

The effectiveness of orally ingested androstenediol in raising serum testosterone concentrations may be limited because of hepatic breakdown of the ingested androgens. Because androstenediol administered sublingually with cyclodextrin bypasses first-pass hepatic catabolism, we evaluated the acute hormonal response to sublingual cyclodextrin androstenediol supplement in young men. Eight men (22.9 +/- 1.2 yr) experienced in strength training consumed either 20 mg androstenediol in a sublingual cyclodextrin tablet (Sl Diol) or placebo (Pl) separated by at least 1 wk in a randomized, double-blind, crossover manner. Blood samples were collected before supplementation and at 30-min intervals for 3 h after supplementation. Serum hormone concentrations did not change with Pl. Serum androstenedione concentrations were increased (P < 0.05) above baseline (11.2 +/- 1.1 nmol/l) with Sl Diol from 60 to 180 min after intake and reached a peak concentration of 25.2 +/- 2.9 nmol/l at 120 min. Serum free testosterone concentrations were increased from 86.2 +/- 9.1 pmol/l with Sl Diol from 30 to 180 min and reached a peak concentration of 175.4 +/- 12.2 pmol/l at 60 min. Serum total testosterone concentrations increased above basal (25.6 +/- 2.3 nmol/l) from 30 to 180 min with Sl Diol and reached a peak concentration of 47.9 + 2.9 nmol/l at 60 min. Serum estradiol concentrations were elevated (P < 0.05) above baseline (0.08 +/- 0.01 nmol/l) from 30 to 180 min with Sl Diol and reached 0.14 +/- 0.02 nmol/l at 180 min. These data indicate that sublingual cyclodextrin androstenediol intake increases serum androstenedione, free testosterone, total testosterone, and estradiol concentrations.
Measuring disease activity and functional status in patients with scleroderma and Raynaud's phenomenon (2002)

Our findings demonstrate that the significant activity, disability, pain, and psychological impact of RP and digital ulcers in SSc can be measured by a small set of valid and reliable outcome measures. These outcome measures provide information beyond the quantitative metrics of RP attacks. We propose a core set of measures for use in clinical trials of RP in SSc patients that includes the RCS, patient and physician VAS ratings of RP activity, a digital ulcer/infarct measure, measures of disability and pain (HAQ), and measures of psychological function (AIMS2).
The End of the Outsourcing Era (1996)

It’s 2020, and IS enterprises have emerged from their relationship with outsourcing a bit wiser. Why did the relationship seem so promising, and what made the parties go their separate ways? Is there hope for a reconciliation?
TOTAL SPONDYLECTOMY IN CHONDROSARCOMA ARISING FROM THE SEVENTH THORACIC VERTEBRA (1971)

1. A forty-nine-year-old man had a chondrosarcoma arising from the body of the seventh thoracic vertebra. The tumour protruded into the mediastinum and also into the spinal canal where it displaced the spinal cord. 2. At operation all the seventh thoracic vertebra and parts of the sixth and eighth were removed together with the tumour. The thoracic spine was reconstructed by inserting two iliac bone-blocks between the cut bodies of the sixth and eighth vertebrae and by wiring two strong "A. O." plates to the transverse processes of the third to the sixth and the eighth to the tenth vertebrae. 3. The patient was nursed in a plaster-of-Paris bed for three and a half months. 4. One year and three months after operation, the patient was walking and well, with no signs of recurrence or metastasis. Radiographs showed that a block-vertebra had been created from the iliac grafts and the two cut vertebrae.
A full-bridge zero-voltage-switched multi-resonant converter for pulse-load applications (1990)

The zero-voltage-switching multiresonant technique was used to fabricate a full-bridge converter for high-power, pulse-load applications. The complex operation of the full-bridge zero-voltage switched multiresonant converter is analyzed. The converter can operate in four modes, each representing a specific topological sequence. The DC voltage conversion-ratio characteristics encompassing all possible operating modes are derived. These characteristics are used to define a complete, step-by-step design procedure which minimizes the conduction loss in the primary side of the power stage by minimizing the RMS currents through the switches. The design procedure was used to fabricate a converter that operates at an input voltage of from 220 to 350 V and delivers 32 V to pulse load, with a peak current of 45 A. The maximum efficiency of the converter was measured at 90.5%.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
SmartEscape: A Mobile Smart Individual Fire Evacuation System Based on 3D Spatial Model (2018)

We propose SmartEscape, a real-time, dynamic, intelligent and user-specific evacuation system with a mobile interface for emergency cases such as fire. Unlike past work, we explore dynamically changing conditions and calculate a personal route for an evacuee by considering his/her individual features. SmartEscape, which is fast, low-cost, low resource-consuming and mobile supported, collects various environmental sensory data and takes evacuees’ individual features into account, uses an artificial neural network (ANN) to calculate personal usage risk of each link in the building, eliminates the risky ones, and calculates an optimum escape route under existing circumstances. Then, our system guides the evacuee to the exit through the calculated route with vocal and visual instructions on the smartphone. While the position of the evacuee is detected by RFID (Radio-Frequency Identification) technology, the changing environmental conditions are measured by the various sensors in the building. Our ANN (Artificial Neural Network) predicts dynamically changing risk states of all links according to changing environmental conditions. Results show that SmartEscape, with its 98.1% accuracy for predicting risk levels of links for each individual evacuee in a building, is capable of evacuating a great number of people simultaneously, through the shortest and the safest route.
Whole virus influenza vaccine activates dendritic cells (DC) and stimulates cytokine production by peripheral blood mononuclear cells (PBMC) while subunit vaccines support T cell proliferation (1998)

Three types of trivalent influenza vaccines were analysed for their in vitro stimulatory properties on immune cells from young healthy volunteers. A whole inactivated virus (WV) vaccine, a conventional subunit (c-SU) preparation and a new virosomal subunit (v-SU) vaccine were used. Blood-derived DC up-regulated MHC class II, CD54, CD80 and CD86 after exposure to WV vaccine, indicating their functional maturation, but were only moderately affected by subunit (SU) vaccines. In addition, IL-12 and tumour necrosis factor-alpha (TNF-alpha) secretion by DC were markedly enhanced by WV, but not by SU vaccines. The production of IL-2 and interferon-gamma (IFN-gamma) by PBMC was also strongly stimulated by WV, but much less by SU vaccines, among which the v-SU vaccine was a better stimulator of IL-2 secretion. In contrast to WV vaccine both SU vaccines were powerful stimulators of PBMC proliferation. Our results suggest that the presence of influenza core components leads to the activation of DC and triggers the production of cytokines by PBMC. SU vaccines are in contrast excellent stimulators of T cell growth. A combination of WV and SU vaccines in immunization regimes might allow optimal T cell priming as well as the efficient generation and maintenance of memory cells.
From UML sequence diagrams and statecharts to analysable petri net models (2002)

In this paper we study the use of Sequence Diagrams and Statecharts of the Unified Modeling Language (UML) for the validation and the performance evaluation of systems. We assume that the system is specified as a set of Statecharts and that Sequence Diagrams are used to represent "executions of interest". UML lacks a formal semantics and hence it is not possible to apply, directly, mathematical techniques on UML models for system validation. To reach this goal we propose an automatic translation of Statecharts and Sequence Diagrams into Generalized Stochastic Petri Nets, and a composition of the resulting net models suitable for reaching a given analysis goal. The translation into Petri nets is based on the abstract syntax of the UML Collaborations and of the State Machines packages (from which Sequence Diagrams and Statecharts are derived) and the construction of the complete model heavily uses compositionality.
A Bayesian Network Based Approach for Change Coupling Prediction (2008)

Source code coupling and change history are two important data sources for change coupling analysis. The popularity of public open source projects in recent years makes both sources available. Based on our previous research, in this paper, we inspect different dimensions of software changes including change significance or source code dependency levels, extract a set of features from the two sources and propose a Bayesian network-based approach for change coupling prediction. By combining the features from the co-changed entities and their dependency relation, the approach can model the underlying uncertainty. The empirical case study on two medium-sized open source projects demonstrates the feasibility and effectiveness of our approach compared to previous work.
[Clinicopathological study of 212 children with primary focal segmental glomerular sclerosis]. (2010)

FSGS is defined as a clinicopathologic syndrome manifesting proteinuria and focal and segmental glomerular sclerosis with foot process effacement. The location of the sclerosis within the glomeruli proved to have prognostic significance. Collapsing glomerulopathy is the most aggressive variant of FSGS. Compared with other variants, GTL variant may be the best type. Different histologic variants of FSGS have substantial differences in clinical features at the time of biopsy diagnosis and substantial differences in renal outcomes. Prolonged treatment of FSGS-NS with corticosteroids and immune suppressive agents may have some effects in achieving sustained remission and improve prognosis in children.