gebhart/patrepeval huggingface dataset:
Disruption of the monocarboxylate transporter-4-basigin interaction inhibits the hypoxic response, proliferation, and tumor progression (2017)

We have previously shown that glioblastoma stem cells (GSCs) are enriched in the hypoxic tumor microenvironment, and that monocarboxylate transporter-4 (MCT4) is critical for mediating GSC signaling in hypoxia. Basigin is involved in many physiological functions during early stages of development and in cancer and is required for functional plasma membrane expression of MCT4. We sought to determine if disruption of the MCT-Basigin interaction may be achieved with a small molecule. Using a cell-based drug-screening assay, we identified Acriflavine (ACF), a small molecule that inhibits the binding between Basigin and MCT4. Surface plasmon resonance and cellular thermal-shift-assays confirmed ACF binding to basigin in vitro and in live glioblastoma cells, respectively. ACF significantly inhibited growth and self-renewal potential of several glioblastoma neurosphere lines in vitro, and this activity was further augmented by hypoxia. Finally, treatment of mice bearing GSC-derived xenografts resulted in significant inhibition of tumor progression in early and late-stage disease. ACF treatment inhibited intratumoral expression of VEGF and tumor vascularization. Our work serves as a proof-of-concept as it shows, for the first time, that disruption of MCT binding to their chaperon, Basigin, may be an effective approach to target GSC and to inhibit angiogenesis and tumor progression.
Continuous pressure measurements in the pharynx and esophagus during sleep in patients with obstructive sleep apnea syndrome (1992)

Pressure measurements were made preoperatively at six sites in the pharynx and esophagus in 18 obstructive sleep apnea syndrome (OSAS) patients, 3 social snorers, and 6 healthy normal controls. Repeat measurements were made approximately 2 months after uvulopalatopharyngoglossoplasty (UPPGP) in 10 of the OSAS patients. The pressure transducers were contained in a thin silicone tube inserted through one nostril, and measurements were made both in the awake state and throughout a night's sleep. Oxygen saturation was also continuously recorded and, additionally, nasal and oral airflow measurements were made as the postoperative control. The majority of patients had obstruction in more than one pharyngeal segment, and the site(s) of obstruction differed in the awake and sleeping states. After UPPGP, 3 of the patients had neither subjective nor objective evidence of obstruction, while the remaining 7, although subjectively improved, had varying degrees of residual obstruction in the nasopharynx and at the level of the soft palate.
Lung Ultrasonography Score to Evaluate Oxygenation and Surfactant Need in Neonates Treated With Continuous Positive Airway Pressure (2015)

The LUS score is well correlated with oxygenation status in both term and preterm neonates, and it shows good reliability to predict surfactant administration in preterm babies with a GA less than 34 weeks under continuous positive airway pressure.
Initial Observations of Electronic Medical Record Usage During CT and MRI Interpretation: Frequency of Use and Impact on Workflow (2010)

For CT and MRI interpretation, EMR usage is frequent and comprises a significant fraction of diagnostic effort.
Carbon-13 nuclear magnetic resonance studies of organometallic compounds. VII. 1,5-Cyclooctadieneplatinum(II) derivatives (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-13 nuclear magnetic resonance studies of organometallic compounds. VII. 1,5-Cyclooctadieneplatinum(II) derivativesMalcolm H. Chisholm, Howard C. Clark, Leo E. Manzer, J. B. Stothers, and John E. H. WardCite this: J. Am. Chem. Soc. 1975, 97, 4, 721–727Publication Date (Print):February 1, 1975Publication History Published online1 May 2002Published inissue 1 February 1975https://pubs.acs.org/doi/10.1021/ja00837a007https://doi.org/10.1021/ja00837a007research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Acetylation and deacetylation of Cdc25A constitutes a novel mechanism for modulating Cdc25A functions with implications for cancer (2016)

The dual specificity phosphatase Cdc25A is a key regulator of the cell cycle that promotes cell cycle progression by dephosphorylating and activating cyclin-dependent kinases. In response to genotoxicants, Cdc25A undergoes posttranslational modifications which contribute to its proteasome-mediated degradation and consequent cell cycle checkpoint arrest. The most thoroughly studied Cdc25A modification is phosphorylation. We now provide the first evidence that Cdc25A can be acetylated and that it directly interacts with the ARD1 acetyltransferase which acetylates Cdc25A both biochemically and in cultured cells. When acetylated, Cdc25A has an extended half-life. We have also identified the class IV histone deacetylase, HDAC11, as a Cdc25A deacetylase. We further show that DNA damage, such as exposure to methyl methanesulfonate (MMS), etoposide or arsenic, increases Cdc25A acetylation. Importantly, this acetylation modulates Cdc25A phosphatase activity and its function as a cell cycle regulator, and may reflect a cellular response to DNA damage. Since Cdc25A, ARD1, and HDAC11 are frequently dysregulated in multiple types of cancer, our findings may provide insight into a novel mechanism in carcinogenesis.
Stereocontrol in organic synthesis using silicon-containing compounds. A formal synthesis of prostaglandins controlling the stereochemistry at C-15 using a silyl-to-hydroxy conversion following a stereochemically convergent synthesis of an allylsilane (1998)

Hydrosilylation of isoprene with chloro(diphenyl)silane gave (Z)-chloro(2-methylbut-2-enyl)diphenylsilane 7. The cuprate reagent derived from this chloride underwent conjugate addition to methyl cinnamate 11, 1,2-silylcupration with hex-1-yne 16 and allene 18, and allylic displacement reactions with 1-vinylcyclohexyl acetate 20 and (Z)-1-cyclopentyloct-2-en-1-yl acetate 22. The silyl group in each of the products was converted into a hydroxy, with the removal of the 2-methylbut-2-enyl group taking place under much milder acidic conditions than those needed to remove the phenyl group from the dimethyl(phenyl)silyl group, and making this group suitable for the conversion of an allylsilane into an allyl alcohol. A stereospecifically anti conjugate displacement of the allylic benzoate group in (Z)-(1S,5R,6R,7R,1′S)-7-benzoyloxy-6-(1′-benzoyloxyoct-2′-enyl)-2-oxabicyclo[3.3.0]octan-3-one 52, and a stereospecifically syn conjugate displacement of the carbamate group in (Z)-(1S,5R,6R,7R,1′R)-7-benzoyloxy-6-(1′-N-phenylcarbamoyloxyoct-2′-enyl)-2-oxabicyclo[3.3.0]octan-3-one 51, gave stereoconvergently the same allylsilane (1′E,2″Z)-(1S,5R,6R,7R,3′S)-7-benzoyloxy-6-[3′-(2″-methylbut-2″-enyl)diphenylsilyloct-1′-enyl]-2-oxabicyclo[3.3.0]octan-3-one 53. Silyl-to-hydroxy conversion gave the allyl alcohol (E)-(1S,5R,6R,7R,3′S)-7-benzoyloxy-6-(3′-hydroxyoct-1′-enyl)-2-oxabicyclo[3.3.0]octan-3-one 54, having the relative and absolute stereochemistry at C-15 of the prostaglandins.
A Comparative Study of Inference Engines (2010)

Understanding and using the data and knowledge encoded in semantic web documents requires an inference engine. Inference engines, also called reasoners, are software applications that derive new facts or associations from existing information. Inference and inference rules allow for deriving new data from data that is already known. Thus, new pieces of knowledge can be added based on previous ones. By creating a model of the information and relationships, we enable reasoners to draw logical conclusions based on the model. The use of inference engines in the semantic web allows applications to inquire why a particular conclusion has been reached, i.e. semantic applications can give proof of their conclusions. Proof traces or explains the steps involved in logical reasoning. This paper is a survey and study work, which presents a comparison of different types of inference engines in context of semantic web. It will enable to differentiate among different types of inference engines which may be beneficial to realize the various proposed prototype systems with different ideas and views on what an inference engine for semantic web should do.
SegTrack: A novel tracking system with improved object segmentation (2013)

Most tracking methods depend on a rectangle or an ellipse mask to segment and track objects. Typically, using a larger or smaller mask will lead to loss of tracked objects. In this paper, we propose an object tracking system (SegTrack) that deals with partial and full occlusions by employing improved segmentation methods. Our improved mixture of Gaussians segments foreground objects from the background and solves stop-then-move and move-then-stop problems. Then, the KLT tracker tracks objects in consecutive frames and detects partial and full occlusions. In partial occlusion, a novel silhouette segmentation algorithm evolves the silhouettes of occluded objects by matching the location and appearance of occluded objects between successive frames. In full occlusion, one or more feature vectors for each tracked object are used to re-identify the object after reappearing. Our experimental results show that SegTrack provides more accurate and robust tracking when compared to other state-of-the-art trackers.
Digital TV Over Broadband (2001)

The Digital Destiny From Show Biz to Show Bits Digitology 101 HDTV Digital Shape Shifting: The Many Forms of DTV Two-faced TV: The Interactive Promise From Network TV to Networked TV Fiber Fever: The Growth of Global Networks Wired and Wireless Bitpipes Next Generation Networks Living Digitally From Reel (Movies) to Real (Networks) Cache Flow: From Bitstreams to Revenue Streams Brave New WWWorld
Comparison of Step-Scan and Rapid-Scan Approaches to the Measurement of Mid-Infrared Fourier Transform Vibrational Circular Dichroism (1997)

We report mid-infrared Fourier transform vibrational circular dichroism (FT-VCD) spectra obtained by using three different measurement schemes: the traditional sequential rapid-scan method, a new simultaneous rapid-scan method, and the step-scan method. We also provide a description of the optical setup used to obtain the VCD spectra. Using this optical layout, we are able to measure FT-VCD spectra for single enantiomers of the chiral sample with low levels of interfering artifacts and for collection times as fast as one minute. For approximately 20 min of spectral collection, the three FT-VCD measurements approaches tested here yield nearly the same signal quality. The approach with the highest quality, by a small margin, was the simultaneous rapid-scan method. Next was the sequential rapid-scan approach, followed closely by the step-scan method. VCD spectra and noise curves obtained with these different measurement techniques are presented and compared, and their relative advantages and disadvantages are discussed.
Designing and fabricating mechanical automata from mocap sequences (2013)

Mechanical figures that mimic human motions continue to entertain us and capture our imagination. Creating such automata requires expertise in motion planning, knowledge of mechanism design, and familiarity with fabrication constraints. Thus, automaton design remains restricted to only a handful of experts. We propose an automatic algorithm that takes a motion sequence of a humanoid character and generates the design for a mechanical figure that approximates the input motion when driven with a single input crank. Our approach has two stages. The motion approximation stage computes a motion that approximates the input sequence as closely as possible while remaining compatible with the geometric and motion constraints of the mechanical parts in our design. Then, in the layout stage, we solve for the sizing parameters and spatial layout of all the elements, while respecting all fabrication and assembly constraints. We apply our algorithm on a range of input motions taken from motion capture databases. We also fabricate two of our designs to demonstrate the viability of our approach.
Characterization of RO4583298 as a novel potent, dual antagonist with<i>in vivo</i>activity at tachykinin NK<sub>1</sub>and NK<sub>3</sub>receptors (2010)

RO4583298 is a high-affinity, non-competitive, long-acting in vivo NK₁/NK₃ antagonist; hence providing a useful in vitro and in vivo pharmacological tool to investigate the roles of NK₁ and NK₃ receptors in psychiatric disorders.
A framework for aerial inspection of siltation in waterways (2015)

Silt accumulation and sedimentation in canal beds leads to deterioration of watercourses over time. Every year a forced closure of the canals in the Indus basin is inevitable for canal cleaning, entailing a very large scale and costly operation. Silt removal precision is prone to inefficiencies due to subjective decision making in the cleaning process. In this paper, we lay out a theoretical framework to map the semi-structured (emptied) canal bed terrains with an Unmanned Aerial Vehicle (UAV) system for quantitative inspection of deposited silt. The study employs Gaussian process regression on sampled points to determine a continuous distribution of silt surface, thereby, predicting the volume of silt on canal bed. Our theoretical analysis builds upon certain mathematical bounds on the variance of estimated volume, while explicitly considering localization error and sensor noise. Essentially, we setup a framework for studying how tolerable are the process and measurement uncertainties, while achieving a desired accuracy in silt profile and corresponding volume. We demonstrate the regression results in simulations as well as lab-scaled-model (LMS151 laser scanner) with different sets of parameters. Volume estimation is verified practically and mathematical performance limits are proposed in established aerial canal inspection system.
A Simple Method for Imaging <em>Arabidopsis</em> Leaves Using Perfluorodecalin as an Infiltrative Imaging Medium (2012)

The problem of acquiring high-resolution images deep into biological samples is widely acknowledged. In air-filled tissue such as the spongy mesophyll of plant leaves or vertebrate lungs further difficulties arise from multiple transitions in refractive index between cellular components, between cells and airspaces and between the biological tissue and the rest of the optical system. Moreover, refractive index mismatches lead to attenuation of fluorophore excitation and signal emission in fluorescence microscopy. We describe here the application of the perfluorocarbon, perfluorodecalin (PFD), as an infiltrative imaging medium which optically improves laser scanning confocal microscopy (LSCM) sample imaging at depth, without resorting to damaging increases in laser power and has minimal physiological impact. We describe the protocol for use of PFD with Arabidopsis thaliana leaf tissue, which is optically complex as a result of its structure (Figure 1). PFD has a number of attributes that make it suitable for this use. The refractive index of PFD (1.313) is comparable with that of water (1.333) and is closer to that of cytosol (approx. 1.4) than air (1.000). In addition, PFD is readily available, non-fluorescent and is non-toxic. The low surface tension of PFD (19 dynes cm⁻¹) is lower than that of water (72 dynes cm⁻¹) and also below the limit (25-30 dyne cm⁻¹) for stomatal penetration, which allows it to flood the spongy mesophyll airspaces without the application of a potentially destructive vacuum or surfactant. Finally and crucially, PFD has a great capacity for dissolving CO₂ and O₂, which allows gas exchange to be maintained in the flooded tissue, thus minimizing the physiological impact on the sample. These properties have been used in various applications which include partial liquid breathing and lung inflation, surgery, artificial blood, oxygenation of growth media, and studies of ice crystal formation in plants. Currently, it is common to mount tissue in water or aqueous buffer for live confocal imaging. We consider that the use of PFD as a mounting medium represents an improvement on existing practice and allows the simple preparation of live whole leaf samples for imaging.
Magnetic microrheometer for <i>in situ</i> characterization of coating viscosity (2010)

A magnetic microrheometer has been designed to characterize the local viscosity of liquid-applied coatings in situ during solidification. The apparatus includes NdFeB magnets mounted on computer-controlled micropositioners for the manipulation of ∼1 μm diameter superparamagnetic particles in the coating. Magnetic field gradients at 20-70 T/m are generated by changing magnet size and the gap distance between the magnets. A specimen stage located between two magnets is outfitted with a heater and channels to control process conditions (temperature and air flow), and a digital optical microscope lens above the stage is used to monitor the probe particle position. Validation studies with glycerol and polyimide precursor solution showed that microrheometry results match traditional bulk rheometry within an error of 5%. The viscosities of polyvinyl alcohol (PVA) solution and polyimide precursor solution coatings were measured at different shear rates (0.01-5 s(-1)) by adjusting the magnetic field gradient. The effect of proximity to the substrate on the particle motion was characterized and compared with theoretical predictions. The magnetic microrheometer was used to characterize the time-viscosity profile of PVA coatings during drying at several temperatures. The viscosity range measured by the apparatus was 0.1-20 Pa s during drying of coatings at temperatures between room temperature and 80 °C.
Updated guidance for trusted systematic reviews: a new edition of the Cochrane Handbook for Systematic Reviews of Interventions (2019)

On a shelf in the sunny, open-plan o ice of Cochrane Australia in Melbourne, there's a large, white ring-binder that, it's fair to say, hasn't been opened in a while.It's a printed copy of the original, 1994 edition of the Cochrane Collaboration Handbook, edited by Dave Sackett,[1] and within it the original guidance on the methods to be used.The section on preparing and maintaining systematic reviews, edited by Andy Oxman, weighs in at a total of 76 pages.[2
Polydopamine hydrophilic modification of polypropylene separator for lithium ion battery (2014)

ABSTRACT The modified polypropylene (PP) separators with self‐polymerization of dopamine on the surfaces are prepared by a simple solution‐immersion method to improve the interfacial hydrophilic and discharge performance. The contact angle test and the liquid electrolyte uptake capacity test results show that the wettability and the electrolyte‐retention ability of polydopamine‐modified separator are improved significantly. The robust and thin polydopamine layer on the surface also enhances thermal performance and tensile strength of the modified PP separator certified by DSC and tensile strength tests. The ionic conductivity of the modified PP separator is up to 3.08 mS·cm −1 , ∼2.5 times of the bare separator. Good discharge capacity retention and C‐rate discharge performance are demonstrated by a 2025 coin half‐cell with the liquid electrolyte‐soaked polydopamine modified PP separator sandwiched between lithium metal anode and LiFePO 4 cathode. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40543.
Interference in astronomical speckle patterns (1976)

We discuss the experimental conditions for which the resolution limit for astronomical speckle interferometry may be the diffraction limit. We show that the point spread function is not spatially statistically stationary with wavelength across the speckle patterns.
Quantification of cardiolipin molecular species in <i>Escherichia coli</i> lipid extracts using liquid chromatography/electrospray ionization mass spectrometry (2012)

This work characterizes the CL composition of E. coli cells grown at various temperatures. By quantifying CL species at a molecular level we have illuminated the molecular complexity of the CL in this relatively simple model organism. This data will be useful for understanding CL function in E. coli and other organisms.
Using digital maps to enhance lane keeping support systems (2007)

The development of a system that can be used for a safe, reliable, highly available onboard lane keeping support system is a critical research topic. One of the most important functions in driver assistant systems is the detection of unintentional lane departures. Current lane departure warning systems focus mainly in the detection of lane markings using vision sensors, such as CMOS cameras. In order to increase accuracy and robustness of such systems the utilization of digital maps is necessary. The goal of combining camera and map data is to extend the road geometry in further distances and eliminate false alarms based on unintentional maneuvers caused by the driver. The overall system efficiency is increased furthermore by using also vehicle dynamics and road geometry calculated using radar data.
Extraction of High-Level Musical Structure From Audio Data and Its Application to Thumbnail Generation (2006)

A method for segmenting musical audio with a hierarchical timbre model is introduced. New evidence is presented to show that music segmentation can be recast as clustering of timbre features, and a new clustering algorithm is described. A prototype thumbnail-generating application is described and evaluated. Experimental results are given, including comparison of machine and human segmentations
Abnormal splice in a mutant human beta-globin gene not at the site of a mutation. (1983)

We have studied the expression of a cloned mutant human beta-globin gene in tissue culture cells. The gene, which was previously isolated from the chromosomal DNA of an individual with a low level of normal beta-globin expression (beta+-thalassemia), contains five mutations inside the large intervening sequence (IVS2), as well as a silent change in codon 2. This beta-thalassemia gene (thal) was inserted into a plasmid that is replicated and transcribed in a line of monkey kidney cells in culture. S1 nuclease mapping of the beta-globin RNA transcribed from this gene indicates that some of the beta-globin RNA is spliced abnormally by using a cryptic 3' splice sequence normally present in IVS2 but not used in processing the normal beta-globin transcript. The cryptic 3' splice site is not the site of a mutation in the thal gene. Because neither the 5' or 3' splice junction nor the cryptic site is mutated in this gene, it is most likely that the mutation at position 705 of IVS2, the only nonpolymorphic change in the gene, interferes indirectly with normal processing. These results suggest that certain sequences within IVS must be conserved to prevent abnormal splicing and loss of gene function.