gebhart/patrepeval huggingface dataset:
Molecular cloning of human and rabbit UDP-<i>N</i>-acetylglucosamine: α-3-D-mannoside <i>β</i>-1, 2-<i>N</i>-acetylglucosaminyltransferase I (1991)

Conference Article| August 01 1991 Molecular cloning of human and rabbit UDP-N-acetylglucosamine: α-3-D-mannoside β-1, 2-N-acetylglucosaminyltransferase I H. Schachter; H. Schachter *Research Institute, The Hospital for Sick Children, Toronto, Canada Search for other works by this author on: This Site PubMed Google Scholar E. Hull; E. Hull *Research Institute, The Hospital for Sick Children, Toronto, Canada†Department of Biochemistry, University of Toronto, Toronto, Canada Search for other works by this author on: This Site PubMed Google Scholar M. Sarkar; M. Sarkar *Research Institute, The Hospital for Sick Children, Toronto, Canada Search for other works by this author on: This Site PubMed Google Scholar R. J. Simpson; R. J. Simpson ‡Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research and The Walter and Eliza Hall Institute for Medical Research, Melbourne, Australia Search for other works by this author on: This Site PubMed Google Scholar R. L. Moritz; R. L. Moritz ‡Joint Protein Structure Laboratory, Ludwig Institute for Cancer Research and The Walter and Eliza Hall Institute for Medical Research, Melbourne, Australia Search for other works by this author on: This Site PubMed Google Scholar J. W. M. Höppener; J. W. M. Höppener §Institute of Molecular Biology, University of Utrecht, Utrecht, The Netherlands Search for other works by this author on: This Site PubMed Google Scholar R. Dunn R. Dunn ‖Montreal General Hospital, Montreal, Canada Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1991) 19 (3): 645–648. https://doi.org/10.1042/bst0190645 Article history Received: April 09 1991 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation H. Schachter, E. Hull, M. Sarkar, R. J. Simpson, R. L. Moritz, J. W. M. Höppener, R. Dunn; Molecular cloning of human and rabbit UDP-N-acetylglucosamine: α-3-D-mannoside β-1, 2-N-acetylglucosaminyltransferase I. Biochem Soc Trans 1 August 1991; 19 (3): 645–648. doi: https://doi.org/10.1042/bst0190645 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: GnT, N-acetylglucosaminyltransferase, RP-h.p.l.c, reversed-phase h.p.l.c. This content is only available as a PDF. © 1991 Biochemical Society1991 Article PDF first page preview Close Modal You do not currently have access to this content.
The design of multimedia Web-based phone and billing system with freeware over the VoIP network (2006)

In VoIP networks, SIP encourages the convergence of telephony and Web-based services. The Web phone has become an attractive tool for developing VoIP services. In this paper, we use open source software to build VoIP platform providing the Web phone, voice, video, IM, and prepaid services. A billing system and Web pages are also developed for the administrator providing the administrative functions. The VoIP users can also see their call detail records in real time. Experimentations are conducted showing that our system can accommodate 400 calls per second without loss. If the bottleneck component is moved to a standalone server, this number is nearly doubled.
Regulation and role of guanylate cyclase-cyclic GMP in vascular relaxation. (1987)

Three classes of vasodilators mediate their effects through the activation of guanylate cyclase and the increased synthesis of cyclic GMP. Nitrovasodilators such as nitroglycerin, nitroprusside, hydroxylamine, azide, etc. result in the generation of the nitric oxide free radical that activates the cytosolic (soluble) isoenzyme form of guanylate cyclase. These agents have been useful in increasing cyclic GMP synthesis in numerous model systems and these effects are independent of extracellular calcium. The increased synthesis of cyclic GMP and the activation of cyclic GMP-dependent protein kinase result in the altered phosphorylation of many smooth muscle proteins including the dephosphorylation of myosin light chain, which is associated with vascular and tracheal smooth muscle relaxation. These latter effects may result from cyclic GMP decreasing cytosolic free calcium concentrations and the activity of myosin light chain kinase. Another class of vasodilators, designated endothelium-dependent vasodilators, includes a long list of agents such acetylcholine, histamine, A23187, ATP, thrombin, etc. that relax vessels only when the endothelium is intact. These agents result in the increased endothelial synthesis and/or release of a factor(s) designated endothelial-derived relaxant factor (EDRF), the structure of which is unknown. This labile factor also activates the soluble isoenzyme form of guanylate cyclase in the smooth muscle resulting in cyclic GMP accumulation and the same cascade of events as above. There is evidence that even under basal, non-stimulated conditions there is EDRF release that influences vascular tone due to the increased synthesis of cyclic GMP. A third class of vasodilators, atrial natriuretic factor (ANF) or atriopeptins, includes a family of peptides that are produced in cardiac atria and other tissues and influence cardiovascular volume and dynamics by causing natriuresis, diuresis, vasodilation and decreased renin, aldosterone and vasopressin secretion. These peptide hormones also increase cyclic GMP synthesis in vascular, renal, adrenal and other tissues. These effects are mediated through specific ANF receptors that couple to and activate the membrane (particulate) isoenzyme form of guanylate cyclase and increase cyclic GMP-dependent protein kinase activity. There are two ANF receptor subtypes in most cells and tissues that are 130,000 and 66,000 daltons. The ANF receptor of about 130,000 daltons, designated receptor ANF-R1 copurifies with particulate guanylate cyclase through numerous procedures and may be part of the membrane-associated guanylate cyclase complex.(ABSTRACT TRUNCATED AT 400 WORDS)
[Isolation, cultivation and characterisation of stem cells in human periodontal ligament]. (2008)

Recent studies have revealed the presence of postnatal stem cells in tissues of dental origin. Our objective was to establish a standardized in vitro model system to investigate periodontal regenerative procedures for potential clinical application. We aimed to prepare primary cell cultures from human periodontal ligament and to identify clonogenic progenitor cells. After scraping PDL tissue from extracted wisdom teeth, the extracellular matrix was enzymatically degraded to obtain isolated cells for culturing. The effect of FCS and Emdogain on cell viability of the cultures was estimated by MTT-assay. Cell populations expressing STRO-1 mesenchymal, c-kit embryonic and CD34 hematopoietic stem cell markers were identified by FACS-analysis. We successfully established primary cell cultures from the human PDL. The proliferation rate of the cultures was enhanced by the supplementation of the culture medium by serum or Emdogain. The PDL cultures contained cells capable of colony-formation, as well as cells with STRO-1, c-kit and CD-34 expression. The primary cultures were maintained through multiple passages. These findings present a novel opportunity to further investigate the differentiation and proliferation of PDL derived cells potentially capable of periodontal regeneration.
Recognizing user communicative intention in a consultant system with a natural language interface (2003)

This paper defines communicative intentions instead of illocutionary acts defined in the speech act theory, and proposes a method of recognizing them from user utterances. Although the speech act theory deals with the complex mental states of humans, similar complexity is not necessary for an effective interface between humans and machines, whose relation is modelled as that of master and slave. The authors reduce the complexity so that the communicative intentions can be recoognized easily.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The excitatory synaptic action of climbing fibres on the Purkinje cells of the cerebellum (1966)

1. A single climbing fibre makes an extraordinarily extensive synaptic contact with the dendrites of a Purkinje cell. Investigation of this synaptic mechanism in the cerebellum of the cat has been based on the discovery by Szentagothai & Rajkovits (1959) that the climbing fibres have their cells of origin in the contralateral inferior olive.2. Stimulation in the accessory olive selectively excites fibres that have a powerful synaptic excitatory action on Purkinje cells in the contralateral vermis, evoking a repetitive spike discharge of 5-7 msec duration. Almost invariably this response had an all-or-nothing character. In every respect it corresponds with the synaptic action that is to be expected from climbing fibres.3. Intracellular recording from Purkinje cells reveals that this climbing fibre stimulation evokes a large unitary depolarization with an initial spike and later partial spike responses superimposed on a sustained depolarization.4. Typical climbing fibre responses can be excited, but in a much less selective manner, by stimulation of the olive-cerebellar pathway in the region of the fastigial nucleus, there being often a preceding antidromic spike potential of the Purkinje cell under observation.5. Impaled Purkinje cells rapidly deteriorate with loss of all spike discharge, the climbing fibre response being then reduced to an excitatory post-synaptic potential. This potential shows that stimulation of the inferior olive may evoke two or more discharges at about 2 msec intervals in the same climbing fibre. The complexity of neuronal connexions in the inferior olive is also indicated by the considerable latency range in responses.6. A further complication is that, with stimulation in the region of the fastigial nucleus, the initial direct climbing fibre response is often followed by a reflex discharge, presumably from the inferior olive, which resembles the responses produced by inferior olive stimulation in being often repetitive.7. Typical climbing fibre responses have been evoked by peripheral nerve stimulation and frequently occur spontaneously.8. An account is given of the way in which the responses evoked by climbing fibres in the individual Purkinje cells can account for the potential fields that an inferior olive stimulus evokes on the surface and through the depth of the cerebellar cortex.9. By the application of currents through the recording intracellular electrode it has been possible to effect large changes in the excitatory post-synaptic potential produced by a climbing fibre, it being diminished and even reversed with depolarizing currents and greatly increased by hyperpolarizing currents.
Retrieval of particle size distribution in the dependent model using the moment method (2007)

The problem of determining particle size distribution using the moment method in the spectral extinction technique is studied. The feasibility and reliability of the retrieval of spherical particle size distribution using the moment method are investigated. The single spherical particle extinction efficiency, which is derived theoretically using the Mie's solution to Maxwell's equation, is approximated with a higher order polynomial in order to apply the moment method. Simulation and experimental results indicate that a fairly reasonable representation of the particle size distribution can be obtained using the moment method in the dependent model algorithm. The method has advantages of simplicity, rapidity, and suitability for in-line particle size measurement.
Sperm surface galactosyltransferase activities during in vitro capacitation (1982)

Studies using genetic and biochemical probes have suggested that mouse sperm surface galactosyltransferases may participate during fertilization by binding N- acetylglucosamine (GlcNAc) residues in the egg zona pellucida. In light of these results, we examined sperm surface galactosyltransferase activity during in vitro capacitation to determine whether changes in enzymatic activity correlated with fertilizing ability. Results show that surface galactosyltransferases on uncapacitated sperm was preferentially loaded with poly N-acetyllactosamine substrates. As a consequence of capacitation in Ca(++)-containing medium, these polylactosaminyl substrates are spontaneously released from the sperm surface, thereby exposing the sperm galactosyltransferase for binding to the zona pellucida. Sperm capacitation can be mimicked, in the absence of Ca(++), either by washing sperm in Ca(++)-free medium, or by pretreating sperm with antiserum that reacts with the galactosyltransferase substrate. In both instances, sperm galgactosylation of endogenous polylactosaminyl substrates is reduced, coincident with increased galactosylation of exogenous GlcNAc, and increased binding to the zona pellucida. Binding of capacitated sperm to the egg can be inhibited by pronase-digested high molecular weight polyactosaminyl glycoside extracted from epidymal fluids or from undifferentiated F9 embryonal carninoma cells. Thus, these glycosides function as "decapacitation factors" when added back to in vitro fertilization assays. These glycoside "decapacitation factors" inhibit sperm-egg binding by competeing for the sperm surface galactosyltransferase, since (a) they are galactosylated by sperm in the presence of UDP[(3)H]galactose, and (b) enzymatic removal of terminal GlcNAc residues reduces "decapacitation factio" competition. On the other hand "conventional" low molecular weight glycosides, isolated from either epididymal fluid or differentiated F9 cells, fail to inhibit capacitated sperm binding to the zona pellucida. These results define a molecular mechanism for one aspect of sperm capacitation, and help explain why removal of "decapacitation factos" is a necessary prerequisite for sperm binding to the zona pellucida.
Elastic-scattering spectroscopy for cancer detection: What have we learned from preliminary clinical studies? (2004)

Several preliminary clinical studies have been carried out over the past several years to test proof-of-concept of fiber-mediated elastic-scattering spectroscopy for real-time, minimally- invasive diagnosis of cancer. This promising technique is now entering the translational research stage, with statistically larger clinical studies, to determine the efficacy of prospective diagnosis.
Essential role of <i>Helicobacter pylori</i>γ‐glutamyltranspeptidase for the colonization of the gastric mucosa of mice (1999)

Constitutive expression of gamma-glutamyltranspeptidase (GGT) activity is common to all Helicobacter pylori strains, and is used as a marker for identifying H. pylori isolates. Helicobacter pylori GGT was purified from sonicated extracts of H. pylori strain 85P by anion exchange chromatography. The N-terminal amino acid sequences of two of the generated endo-proteolysed peptides were determined, allowing the cloning and sequencing of the corresponding gene from a genomic H. pylori library. The H. pylori ggt gene consists of a 1681 basepair (bp) open reading frame encoding a protein with a signal sequence and a calculated molecular mass of 61 kDa. Escherichia coli clones harbouring the H. pylori ggt gene exhibited GGT activity at 37 degrees C, in contrast to E. coli host cells (MC1061, HB101), which were GGT negative at 37 degrees C. GGT activity was found to be constitutively expressed by similar genes in Helicobacter felis, Helicobacter canis, Helicobacter bilis, Helicobacter hepaticus and Helicobacter mustelae. Western immunoblots using rabbit antibodies raised against a His-tagged-GGT recombinant protein demonstrated that H. pylori GGT is synthesized in both H. pylori and E. coli as a pro-GGT that is processed into a large and a small subunit. Deletion of a 700 bp fragment within the GGT-encoding gene of a mouse-adapted H. pylori strain (SS1) resulted in mutants that were GGT negative yet grew normally in vitro. These mutants, however, were unable to colonize the gastric mucosa of mice when orally administered alone or together (co-infection) with the parental strain. These results demonstrate that H. pylori GGT activity has an essential role for the establishment of the infection in the mouse model, demonstrating for the first time a physiological role for a bacterial GGT enzyme.
Shaykh Mâl'aynîn et les composantes de l'identité musulmane dans l'ouest saharien (fin 19e-début 20e siècles)", in Pratiques et stratégies identitaires au Sahara, Rabat, Publications de l'Institut des Etudes Africaines, 2001, 153-190 (2011)

El presente texto es un resumen del artículo de C. Hamesen el que, a través de un análisis detallado de la personalidad de Chej Maelainin, nos muestra las formas de identidad musulmana presentes en la figura del Chej. En este análisis constata que algunas de estas identidades son una constante en el noroeste africano, el dirigente de la cofradía, el personaje santo y el santo guerrero, pero apunta una dimensión que no se ha tratado apenas, la del sabio musulmán. Una cuestión pendiente que es preciso descubrir.AbstractThis text is a summary of the article by C. Hames in which, through a detailed analysis of the personality of Chej Maelainin, differents forms of Muslim identity related to the figure of Cheij are shown . This analysis concludes that some of these identities are constant in northwest part of Africa, the leader of the brotherhood, the holy figure and holy warrior, but points to a dimension that has not been studied, the wise Muslim. One outstanding question to be discovered.
Orthogonal frequency division multiplexing for indoor optical wireless communications using visible light LEDs (2008)

Visible light LEDs are being used for indoor optical wireless systems as well as room lighting. In indoor diffuse optical wireless links multipath dispersion limits the maximum transmission data rates. In this paper we investigate OFDM system where multipath induced intersymbol interference (ISI) is reduced, enabling higher data rates. Pilot signals are uniformly inserted into data symbols (subcarriers) and are extracted at the receiver for channel estimation. Predicted and simulated results for the symbol error rate (SER) for an OFDM employing BPSK, QPSK and M-QAM for line of sight (LOS) and diffuse links are presented for a range of pilot signal.
Thermal oxidative degradation of isotactic polypropylene catalyzed by copper and copper oxide interfaces (1979)

Abstract The oxidative degradation of isotactic polypropylene films coated on well‐defined Cu(Cu 2 O), CuO 0.67 , and CuO films in a temperature range of 90–120°C in a quartz‐spoon‐gauge‐reaction vessel was studied. This catalytic reaction has been compared with the oxidation of polypropylene without copper or oxide films. The reaction vessel contained, if needed, P 2 O 5 and/or KOH as “getters” for H 2 O and CO 2 , these substances could be menitored continuously. Cu(Cu 2 O) films were transformed during oxidation of the polymer to yellow CuO 0.67 below 100°C and above this temperature to black CuO in the presence of H 2 O and CO 2 , whereas in the absence of these compounds CuO was formed below 100°C and CuO 0.67 at 120°C. Characteristic autoxidation curves obtained in the absence of H 2 O and CO 2 showed induction periods that were shorter for copper oxide‐polymer interfaces than for glass‐polymer interfaces (i.e., for uncatalyzed oxidation). Abnormalities were observed for Cu(Cu 2 O)‐polymer interfaces because of further oxidation of Cu during the reaction. The rates of oxygen consumption were faster for CuO 0.67 ‐polymer and CuO‐polymer than for the uncatalyzed reaction; the catalytic action of CuO 0.67 was somewhat larger than that of CuO. The important observation was made that the mechanism of oxidation is not the same in the absence and presence of reaction products; that is, H 2 O and CO 2 . This was confirmed by ion beam scattering experiments, which also revealed that an oxidation‐reduction process takes place at Cu and their oxide interfaces. A mechanism for the catalytic oxidation process, based on the ease by which copper ions are released from the metal oxides at the interface, was formulated. These ions diffuse subsequently as actions of carboxylate anions into the bulk of the polymer. Arrhenius equations of oxygen consumption are given for all cases; the energy of activation calculated for the initiation of the uncatalyzed oxidation agrees with its literature value. The energy of activation for the initiation of the catalyzed reaction was a few kilocalories lower than that for the uncatalyzed reaction. Catalytic action is mainly operative for the initiation reaction at the interface and for the decomposition of hydroperoxides by copper ions. Preventing the delivery of copper ions to the polymer would be the most efficient way of inhibiting the catalysis.
Development of High Resolution Molecular Resist Based on Tris((hydroxypheny)pheny)benzene (2010)

We developed a new chemically amplified molecular resist that showed high resolution by introducing an efficient acid-leaving group to 1,3,5-Tris(p-(p- hydroxyphenyl) phenyl) benzene (THTPPB). The lithographic properties such as sensitivity, developing rate, and adhesion are considered to be controlled using a suitable acid-leaving group. A molecular resist of THTPPB to which is attached with an alicyclic acid-leaving group, hyperlactyl group (HPVE) showed a high resolution for electron beam (EB) lithography and good etch resistance. Half-pitch (hp) 36 nm L&S (1:1) positive pattern was fabricated using 100 keV EB with a new molecular resist based on HPVETPPB.
Suppression of Inflammatory Reactions by Terpinen-4-ol, a Main Constituent of Tea Tree Oil, in a Murine Model of Oral Candidiasis and Its Suppressive Activity to Cytokine Production of Macrophages <i>in Vitro</i> (2013)

The onset of oral candidiasis is accompanied by inflammatory symptoms such as pain in the tongue, edema or tissue damage and lowers the quality of life (QOL) of the patient. In a murine oral candidiasis model, the effects were studied of terpinen-4-ol (T-4-ol), one of the main constituents of tea tree oil, Melaleuca alternifolia, on inflammatory reactions. When immunosuppressed mice were orally infected with Candida albicans, their tongues showed inflammatory symptoms within 24 h after the infection, which was monitored by an increase of myeloperoxidase activity and macrophage inflammatory protein-2 in their tongue homogenates. Oral treatment with 50 µL of 40 mg/mL terpinen-4-ol 3h after the Candida infection clearly suppressed the increase of these inflammatory parameters. In vitro analysis of the effects of terpinen-4-ol on cytokine secretion of macrophages indicated that 800 µg/mL of this substance significantly inhibited the cytokine production of the macrophages cultured in the presence of heat-killed C. albicans cells. Based on these findings, the role of the anti-inflammatory action of T-4-ol in its therapeutic activity against oral candidiasis was discussed.
Optimized coupling of a Gaussian beam into an optical waveguide with a grating coupler: comparison of experimental and theoretical results (1997)

The coupling efficiency of grating couplers is derived for a Gaussian incident beam. Its optimum value depends on the beam waist and on the position of a light spot with respect to the coupler edge for given grating parameters. The characteristic coupling length has been experimentally determined for the grating coupler studied. Relative measurements of the coupling efficiency as a function of incident beam characteristics are in good agreement with the numerical results.