gebhart/patrepeval huggingface dataset:
Evaluation of Immunological Responses in Patients with Ovarian Cancer Treated with the Anti-idiotype Vaccine ACA125 by Determination of Intracellular Cytokines—A Preliminary Report (1999)

In a first clinical trial, 45 patients with advanced ovarian carcinoma and recurrences were treated with the murine monoclonal anti-idiotypic antibody (Ab2) designated ACA125 for active immunotherapy. The monoclonal antibody (MAb) ACA125 mimics a specific epitope of the tumor-associated antigen CA125 expressed by most malignant ovarian tumors. Patients with CA125-positive tumors are immunologically tolerant to CA125, which could be overcome by the use of an anti-idiotypic antibody as a surrogate for the tumor antigen CA125. An immunological response to the anti-idiotype ACA125 in these patients was associated with a statistically significant survival prolongation. Humoral immunity to ACA125 was assessed by induction of anti-anti-idiotypic antibodies (Ab3) directed against CA125. Using flow cytometric detection methods we observe alterations of the intracellular cytokines IFN-gamma, IL-2, and IL-4 at the single-cell level during the course of immunization. There was a strong increase of intracellular IFN-gamma and IL-2 characteristic for a Th1 cell type immune response after treatment with ACA125. A delayed induction of Th2 type response, which promotes antibody-mediated immunity by B cells, could also be detected. The understanding of the kinetics of Th1 and Th2 responses could be important to improve treatment schedules for effective immunotherapy with anti-idiotype vaccines.
Antisense Phosphorodiamidate Morpholino Oligomer Length and Target Position Effects on Gene-Specific Inhibition in<i>Escherichia coli</i> (2004)

Phosphorodiamidate morpholino oligomers (PMOs) are synthetic DNA analogs that inhibit gene expression in a sequence-dependent manner. PMOs of various lengths (7 to 20 bases) were tested for inhibition of luciferase expression in Escherichia coli. Shorter PMOs generally inhibited luciferase greater than longer PMOs. Conversely, in bacterial cell-free protein synthesis reactions, longer PMOs inhibited equally or more than shorter PMOs. Overlapping, isometric (10-base) PMOs complementary to the region around the start codon of luciferase inhibited to different extents in bacterial cell-free protein expression reactions. Including the anti-start codon in PMOs was not required for maximal inhibition. PMOs targeted to 5' nontranslated or 3' coding regions within luciferase mRNA did not inhibit, except for one PMO targeted to the ribosome-binding site. Inhibition of luciferase expression correlated negatively with the predicted secondary structure of mRNA regions targeted by PMO but did not correlate with C+G content of targeted regions. The effects of PMO length and position were corroborated by using PMOs (6 to 20 bases) targeted to acpP, a gene required for viability. Because inhibition by PMOs of approximately 11 bases was unexpected based on previous results in eukaryotes, we tested an 11-base PMO in HeLa cells and reticulocyte cell-free protein synthesis reactions. The 11-base PMO significantly inhibited luciferase expression in HeLa cells, although less than did a 20-base PMO. In reticulocyte cell-free reactions, there was a trend toward more inhibition with longer PMOs. These studies indicate that strategies for designing PMOs are substantially different for prokaryotic than eukaryotic targets.
A novel palladium-catalyzed synthesis of 2-arylbenzimidazoles (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA novel palladium-catalyzed synthesis of 2-arylbenzimidazolesRobert J. Perry and B. David WilsonCite this: J. Org. Chem. 1993, 58, 25, 7016–7021Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/jo00077a019https://doi.org/10.1021/jo00077a019research-articleACS PublicationsRequest reuse permissionsArticle Views1009Altmetric-Citations122LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Antimicrobial Effects and Resistant Regulation of Magnolol and Honokiol on Methicillin-Resistant<i>Staphylococcus aureus</i> (2015)

The antimicrobial killing activity toward methicillin-resistant Staphylococcus aureus (MRSA) has been a serious emerging global issue. In a continuing search for compounds with antibacterial activity against several microorganisms including S. aureus and MRSA, an n-hexane extract of Magnolia officinalis was found to contain magnolol. This compound exhibited potent activity against S. aureus, standard methicillin-susceptible S. aureus (MSSA), and MRSA as well as clinical MRSA isolates. When combined with oxacillin, the antibacterial activities of magnolol and honokiol against the MRSA strain were increased compared to single treatment without antibiotics at 10 µg/mL and 25 µg/mL, respectively. These activities of magnolol and honokiol were dose dependent. Also, magnolol showed synergistic effects with oxacillin against 13 clinical isolates of MRSA. It was determined that magnolol and honokiol had a synergistic effect with oxacillin against MRSA strain. Furthermore, the magnolol inhibited the expression of the resistant genes, mecA, mecI, femA, and femB, in mRNA. We concluded that the antibacterial activity of magnolol against MRSA strain is more related to the mecI's pathway and components of the cell wall than mecR1. Therefore, the results obtained in this study suggest that the combination of magnolol and antibiotics could lead to the development of new combination antibiotics against MRSA infection.
Microporous Hydrophobic Hollow Fiber Modules for Gas-Liquid Phase Separation in Microgravity (1993)

<div class="htmlview paragraph">Gas-liquid interphase mass transfer operations, such as gas-liquid phase separation, gas absorption into liquid or dissolved gas separation from liquid, gas humidification and drying via liquid contact, and evaporative cooling are readily accomplished on the Earth with settling/spray chambers, packed towers, or bubble columns. The inability of these gravity-dependent phase contact and/or separation devices to function in microgravity has almost completely precluded the use of gas-liquid mass transfer as a available unit operation in spacecraft and spacesuit fluid processing systems. Recent advances in hollow fiber membrane performance have made this technology suitable for gas-liquid mass transfer operations in microgravity, in which the walls of many small hollow fibers provide very high volume-specific-surface-area for fluid phase separation or contact without gravity dependence or phase intermixing. This paper reports on gas-water mass transfer tests performed utilizing microporous hydrophobic Hollow Fiber Modules (HFMs) of the type currently employed as blood oxygenators in heart-lung machines. In these HFMs, gases are transferred to and from water or other hydrophilic liquids through the microporous fiber walls; liquid water does not enter the pores of the highly hydrophobic wall material. The experiments included air-water phase separation, absorption of oxygen and carbon dioxide into water and separation of these dissolved gases from water, air humidification and drying by contact with temperature-controlled water, and controlled evaporation of water into vacuum. In each of these experiments, a small, light HFM successfully performed the mass transfer function, with no leakage of liquid water through the porous walls of the hollow fibers, even with high pressure across the fiber walls for extended periods of time. These results demonstrate that gas-liquid mass transfer unit operations on hydrophilic liquids, implemented with microporous hydrophobic HFM technology, are ready for use in microgravity fluid processing systems.</div>
Nitrogen incorporation in diamond films homoepitaxially grown by chemical vapour deposition (2000)

Photoluminescence, optical absorption and electron spin-resonance results are reported for diamond films homoepitaxially grown by chemical vapour deposition on ⟨100⟩-oriented natural IIa diamond. Measured dependencies of the corresponding signals on the growth temperature in the range 950-2100 °C suggest that while most of the nitrogen is present in our films as single atoms, a small part (about 10-3 of all of the nitrogen) could be incorporated as N2 molecules.
Deposition and Plasma Measurements of Zr-Oxide Films with Low Impurity Concentrations by Remote PEALD (2005)

Zr-oxide film was deposited by remote plasma-enhanced atomic layer deposition (PEALD) and showed relatively low impurity contamination. In the Zr-oxide film deposition process, the plasma diagnostics were performed to investigate the effects and characteristics of remote plasma. The carbon contents in Zr-oxide film were decreased with increasing rf power. The electron density and optical emission of species were measured using cutoff and optical emission spectroscopy during the deposition. The electron density in remote plasma was about to The dominant emission species of remote plasma was the excited atomic and molecular oxygen. © 2005 The Electrochemical Society. All rights reserved.
CEA-Directed Second-look Surgery in the Asymptomatic Patient after Primary Resection of Colorectal Carcinoma (1985)

Since 1971, serial carcinoembryonic antigen (CEA) levels have been measured to monitor patients after primary resection of colorectal cancer. Based solely on a rise in CEA level above the baseline established after primary resection, 146 patients were readmitted to the hospital. Chest films, liver-spleen scan, colonoscopy, bone scan, abdominal and pelvic CAT scan, and hepatic arteriograms were performed, and elevated CEA levels were confirmed before reexploration was undertaken. In the 146 patients, 139 (95%) had recurrences, and 81 (58%) of these were resectable for potential cure. Two of the first 22 patients re-explored between 1971 and 1975 are still living 11 and 14 years after second look; of 45 patients reoperated upon from 1976 through 1979 and followed for at least 5 years, 14 (31%) are still living. A rise in CEA above the baseline established after primary resection proved to be a sensitive indicator of recurrence and prompted reexploration before symptoms developed. Early alternative therapy was begun in patients with unresectable recurrences.
Mesoscale Origin of the Enhanced Cycling-Stability of the Si-Conductive Polymer Anode for Li-ion Batteries (2014)

Electrode used in lithium-ion battery is invariably a composite of multifunctional components. The performance of the electrode is controlled by the interactive function of all components at mesoscale. Fundamental understanding of mesoscale phenomenon sets the basis for innovative designing of new materials. Here we report the achievement and origin of a significant performance enhancement of electrode for lithium ion batteries based on Si nanoparticles wrapped with conductive polymer. This new material is in marked contrast with conventional material, which exhibit fast capacity fade. In-situ TEM unveils that the enhanced cycling stability of the conductive polymer-Si composite is associated with mesoscale concordant function of Si nanoparticles and the conductive polymer. Reversible accommodation of the volume changes of Si by the conductive polymer allows good electrical contact between all the particles during the cycling process. In contrast, the failure of the conventional Si-electrode is probed to be the inadequate electrical contact.
Nitric Oxide Mediation of Active Immunosuppression Associated With Graft-Versus-Host Reaction (1999)

In the immunosuppression accompanying the lethal systemic graft-versus-host reaction (GVHR) directed against minor histocompatibility antigens in irradiated adult mice, we previously determined that non-T, non-B, L-leucine methyl ester (LME)-sensitive cells were implicated via two different mechanisms: one, which is interferon-gamma (IFN-gamma)-dependent and affects both T-cell proliferative responses and thymus-independent antibody production by CD5(+) B cells; and a second, which is IFN-gamma-independent and affects B-cell proliferative responses. Because IFN-gamma induces the production of nitric oxide (NO), a potent immunosuppressive molecule, we investigated the involvement of NO in the suppression mediated by the LME-sensitive cells. Inducible NO synthase (iNOS) mRNA, iNOS protein, and the stable end products of iNOS pathway, L-citrulline and nitrite, were detected early in GVHR in LME-sensitive spleen cells taken ex vivo and could be amplified in vitro by T and B mitogens. Inhibition of NO production with arginine analogs (aminoguanidine, N(G)-monomethyl-L-arginine [LMMA]), like anti-IFN-gamma antibodies, reversed suppression of both T-cell responses to concanavalin A and CD5(+) B-cell responses, but not of B-cell response to lipopolysaccharides (LPS). The GVHR-associated, IFN-gamma-dependent immunosuppression of T-cell proliferation and of antibody synthesis by CD5(+) B cells is the consequence of NO production by LME-sensitive cells. Immunohistochemical analyses indicate that these cells belong to the macrophage lineage.
Preparation of porous aromatic framework/ionic liquid hybrid composite coated solid-phase microextraction fibers and their application in the determination of organochlorine pesticides combined with GC-ECD detection (2015)

A novel hybrid material incorporating porous aromatic frameworks and an ionic liquid, 1-(triethoxy silyl)propyl-3-aminopropyl imidazole hexafluorophosphate, was prepared as solid-phase microextraction coating and employed for the extraction of organochlorine pesticides. Combining the advantages of porous aromatic frameworks and an ionic liquid, the fiber exhibited a high adsorption capacity for organochlorine pesticides. Under optimized experimental conditions, enhancement factors of 247-1696 were obtained with good linearity in the range of 1-500 μg L(-1). The detection limits and quantification limits were determined to be in the range of 0.11-0.29 μg L(-1) and 0.35-0.93 μg L(-1). The relative standard deviations for six replicates of organochlorine pesticides were in the range of 4.4%-7.2% and 5.7%-10.1% for one fiber and fiber-to-fiber, respectively. By coupling with a gas chromatography-electron capture detector, the novel fiber was successfully used for the determination of organochlorine pesticides in juice and milk samples with recoveries of 76.1%-121.3%.
MoS<sub>2</sub> Devices Using Pencil Circuits (2015)

As a semiconducting analog of two-dimensional (2D) graphene, MoS 2 monolayer is a three-atom-thick layered transition-metal dichalcogenide, composed of a stratum of molybdenum atoms sandwiched between two layers of sulfur atoms. MoS 2 ’s physical, chemical and opto- electrical properties can be tuned over a wide range by changing its interface and chemistry, opening potential for next generation two-dimensional flexible electronics. Here, we have developed several electronic circuits on paper using MoS 2 and graphite (from pencil) and have studied electrical, capacitive and optoelectrical properties of the flexible composite. Via this process, we envision the evolution of on-demand device fabrication by writing prescribed or innovative ‘scripts’ on paper by combining art and science. Further, such 2D circuitry will evolve into writable, accessible, cost-effective (and disposable) electronics.
A magnetic climbing robot for steel bridge inspection (2014)

Corrosion can cause section loss or cracks in the steel members which is one of the most important causes of deterioration of steel bridges. For some critical components of a steel bridge, it is fatal and could even cause the collapse of the whole bridge. Nowadays the most common approach to steel bridge inspection is visual inspection by inspectors with inspection trucks. This paper mainly presents a climbing robot with magnetic wheels which can move on the surface of steel bridge. Experiment results shows that the climbing robot can move on the steel bridge freely without disrupting traffic to reduce the risks to the inspectors.
A study of the surface structure of decationized Y zeolite by quantitative infrared spectroscopy (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA study of the surface structure of decationized Y zeolite by quantitative infrared spectroscopyThomas Robert Hughes and Harry M. WhiteCite this: J. Phys. Chem. 1967, 71, 7, 2192–2201Publication Date (Print):June 1, 1967Publication History Published online1 May 2002Published inissue 1 June 1967https://pubs.acs.org/doi/10.1021/j100866a035https://doi.org/10.1021/j100866a035research-articleACS PublicationsRequest reuse permissionsArticle Views1618Altmetric-Citations345LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Electrostatic Self‐Assembly of a Pt‐around‐Au Nanocomposite with High Activity towards Formic Acid Oxidation (2010)

Opposites attract: A Pt-around-Au nanocomposite is synthesized by electrostatic self-assembly (see picture; PDDA=poly(diallyldimethylammonium chloride)). This catalyst shows significantly improved activity towards formic acid oxidation versus pure Pt catalysts. The possible reason is the efficient spillover of HCOO from Au to the surrounding Pt nanoparticles, where HCOO is further oxidized to CO2. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
[POSTER] Optimizing Background Subtraction for OST-HMD (2017)

Transparency of optical see-through head-mounted displays (OST-HMDs) makes them suffer from background blending. Existing works have tackled this problem by color correction, but have not addressed how to estimate the background color accurately. In this paper, we apply colorimetric estimation to the subtraction compensation for background blending. Moreover, we propose an optimization framework that trades-off the overall color distortion and clipping to achieve the optimal color correction, constrained to keeping image structures and details.
An Investigation of the Electrode Kinetics and Electrochemistry of Refractory Metal Deposition. (1986)

Electrochemical methods represent important ways in which the refractory metal coatings can be applied to the more active metals and alloys to take advantage, at the new surface, of their superior properties to provide erosion and corrosion protection. Currently, of the refractory metals, only chromium can be plated from aqueous solution, albeit in a form that is not entirely free of cracks and pores. These aberrations in the coating can lead to protection failure under severe conditions. The remaining metals can be plated from molten fluoride electrolytes at temperatures in excess of 700 C. It is clear that less severe operating conditions are required for the plating of these metals, to take advantage of their properties in coatings upon as wide a range of materials as possible. The plating process involves the transport of ionic species, established in an ionic medium, to the solution electrode interface at which these species undergo electron transfer reactions and ligand removal to form metal atoms at favored sites. The aggregation of these atoms into clusters of nuclei, their growth into crystallites, and the accumulation of crystallites into a coherent polycrystalline metal coating, represents the final stages of the complex process of metal plating. This program of work has explored some aspects of each of these contributing processes to develop a scientific basis for the design of new plating baths and techniques which can be operated for chromium and molybdenum at substantially lower temperatures.
Ca2+-stimulated Basal Adenylyl Cyclase Activity Localization in Membrane Lipid Microdomains of Cardiac Sinoatrial Nodal Pacemaker Cells (2008)

Spontaneous, rhythmic subsarcolemmal local Ca(2+) releases driven by cAMP-mediated, protein kinase A (PKA)-dependent phosphorylation are crucial for normal pacemaker function of sinoatrial nodal cells (SANC). Because local Ca(2+) releases occur beneath the cell surface membrane, near to where adenylyl cyclases (ACs) reside, we hypothesized that the dual Ca(2+) and cAMP/PKA regulatory components of automaticity are coupled via Ca(2+) activation of AC activity within membrane microdomains. Here we show by quantitative reverse transcriptase PCR that SANC express Ca(2+)-activated AC isoforms 1 and 8, in addition to AC type 2, 5, and 6 transcripts. Immunolabeling of cell fractions, isolated by sucrose gradient ultracentrifugation, confirmed that ACs localize to membrane lipid microdomains. AC activity within these lipid microdomains is activated by Ca(2+) over the entire physiological Ca(2+) range. In intact SANC, the high basal AC activity produces a high level of cAMP that is further elevated by phosphodiesterase inhibition. cAMP and cAMP-mediated PKA-dependent activation of ion channels and Ca(2+) cycling proteins drive sarcoplasmic reticulum Ca(2+) releases, which, in turn, activate ACs. This feed forward "fail safe" system, kept in check by a high basal phosphodiesterase activity, is central to the generation of normal rhythmic, spontaneous action potentials by pacemaker cells.