gebhart/patrepeval huggingface dataset:
Activation of human papillomavirus type 18 gene expression by herpes simplex virus type 1 viral transactivators and a phorbol ester (1989)

Several viral trans-activators and a tumor promoter were examined for the ability to activate human papillomavirus type 18 (HPV-18) gene expression. A plasmid containing the HPV-18 noncoding region placed upstream of the chloramphenicol acetyltransferase reporter gene was cotransfected with different herpes simplex virus type 1 (HSV-1) genes into several cell lines. Both HSV-1 TIF and ICP0 activated HPV-18 expression; however, activation by TIF was observed only in epithelial cells, while ICP0 stimulated expression in a wide variety of cells. The element activated by both TIF and ICP0 was mapped to a 229-base-pair fragment which also contains an HPV-18 epithelial cell-preferred enhancer. The inclusion of a papillomavirus E2 trans-activator with TIF and ICP0 further increased HPV-18 expression. In contrast, the HSV-1 ICP4 and ICP27 genes, as well as the human T-cell lymphotropic virus type I and human immunodeficiency virus type 1 tat genes, were found to have no effect on HPV-18 expression. In transient assays, the addition of the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) also activated HPV-18 expression. The region of HPV-18 activated by TPA was localized to a sequence which is homologous to other TPA-responsive elements.
Content based video indexing and retrieval (1994)

Video management tools and techniques are based on pixels rather than perceived content. Thus, state-of-the-art video editing systems can easily manipulate such things as time codes and image frames, but they cannot "know," for example, what a basketball is. Our research addresses four areas of content-based video management.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Invasive Cortical Stimulation to Promote Recovery of Function After Stroke (2009)

Future cortical stimulation trials should consider the factors that may have contributed to the peculiar results of the Phase III trial and address those in future study designs.
Adherent Invasive <i>Escherichia coli</i> Strains from Patients with Crohn's Disease Survive and Replicate within Macrophages without Inducing Host Cell Death (2001)

Escherichia coli strains recovered from Crohn's disease (CD) lesions are able to adhere to and invade cultured intestinal epithelial cells. We analyzed the behavior within macrophages of adherent invasive E. coli (AIEC) strains isolated from patients with CD. All the 15 AIEC strains tested were able to replicate extensively within J774-A1 cells: the numbers of intracellular bacteria increased 2.2- to 74.2-fold at 48 h over that at 1 h postinfection. By use of murine peritoneal macrophages and human monocyte-derived-macrophages, the reference AIEC strain LF82 was confirmed to be able to survive intracellularly. Transmission electron micrographs of AIEC LF82-infected macrophages showed that at 24 h postinfection, infected cells harbored large vacuoles containing numerous bacteria, as a result of the fusion of several vacuoles occurring after 8 h postinfection. No lactate dehydrogenase (LDH) release, no sign of DNA fragmentation or degradation, and no binding to fluorescein isothlocyanate-labeled annexin V were observed with LF82-infected J774-A1 cells, even after 24 h postinfection. LF82-infected J774-A1 cells secreted 2.7-fold more tumor necrosis factor alpha (TNF-alpha) than cells stimulated with 1 microg of lipopolysaccharide (LPS)/ml. No release of interleukin-1beta was observed with LPS-prestimulated J774-A1 cells infected with AIEC LF82. These findings showed that (i) AIEC strains are able to survive and to replicate within macrophages, (ii) AIEC LF82 replication does not induce any cell death of the infected cells, and (iii) LF82-infected J774-A1 cells release high levels of TNF-alpha. These properties could be related to some features of CD and particularly to granuloma formation, one of the hallmarks of CD lesions.
On Collision Course: Sir Baldwin Spencer and the Finke River Mission 1894 - 1924 (2010)

The various crises that the Finke River Mission (FRM) in Central Australia has had to face in its 133 year history from 1894-1924 are discussed. The ways in which Sir Baldwin Spencer, a scientist, administrator and anthropologist made life quite difficult for the FRM are highlighted.
Nuclear Substituted 2-Amino-1-(2-pyridyl)propanes<sup>1</sup> (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNuclear Substituted 2-Amino-1-(2-pyridyl)propanes1Alfred Burger and Helen Hu OngCite this: J. Med. Chem. 1963, 6, 2, 205–207Publication Date (Print):March 1, 1963Publication History Published online1 May 2002Published inissue 1 March 1963https://doi.org/10.1021/jm00338a030RIGHTS & PERMISSIONSArticle Views100Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (448 KB) Get e-Alerts Get e-Alerts
Therapeutic Challenges to Retinitis Pigmentosa: From Neuroprotection to Gene Therapy (2011)

Syndromic retinitis pigmentosa (RP) is the result of several mutations expressed in rod photoreceptors, over 40 of which have so far been identified. Enormous efforts are being made to relate the advances in unraveling the patho-physiological mechanisms to therapeutic approaches in animal models, and eventually in clinical trials on humans. This review summarizes briefly the current clinical management of RP and focuses on the new exciting treatment possibilities. To date, there is no approved therapy able to stop the evolution of RP or restore vision. The current management includes an attempt at slowing down the degenerative process by vitamin supplementation, trying to treat ocular complications and to provide psychological support to blind patients. Novel therapeutic may be tailored dependant on the stage of the disease and can be divided in three groups. In the early stages, when there are surviving photoreceptors, the first approach would be to try to halt the degeneration by correction of the underlying biochemical abnormality in the visual cycle using gene therapy or pharmacological treatment. A second approach aims to cope with photoreceptor cell death using neurotrophic growth factors or anti-apoptotic factors, reducing the production of retino-toxic molecules, and limiting oxidative damage. In advanced stages, when there are few or no functional photoreceptors, strategies that may benefit include retinal transplantation, electronic retinal implants or a newly described optogenetic technique using a light-activated channel to genetically resensitize remnant cone-photoreceptor cells.
A chronic intracortical electrode array: preliminary results. (1989)

Two sets of electrode arrays made of either 25- or 50-microns-diameter Teflon-insulated platinum-iridium wire and Teflon have been developed for chronic intracortical electrical stimulation. Cortical histological studies were performed following acute and chronic implantation in cats. While some neural damage resulted from the implantations of either array configuration, a unique set of problems was associated with each diameter wire. Arrays with 50-microns electrodes and lead wires tended to maintain interelectrode spacing upon implantation, but the percutaneous leads retained residual stress which made array implantation difficult. Arrays with 25-microns electrodes and lead wires suffered from changes in interelectrode spacing upon implantation, but were much easier to manipulate during surgery. Both array configurations demonstrated some movement after implantation. It is concluded that a chronic intracortical stimulating electrode array of this geometry should have the following properties: 1) the penetrating electrodes and supporting substrate must be stiff (to maintain interelectrode spacings upon implantation), and 2) the percutaneous leads must be extremely flexible (to avoid array movement after implantation).
Mixed Co−Rh Nitrido-Encapsulated Carbonyl Clusters. Synthesis, Solid-State Structure, and Electrochemical/EPR Characterization of the Anions [Co<sub>10</sub>Rh(N)<sub>2</sub>(CO)<sub>21</sub>]<sup>3-</sup>, [Co<sub>10</sub>Rh<sub>2</sub>(N)<sub>2</sub>(CO)<sub>24</sub>]<sup>2-</sup>, and [Co<sub>11</sub>Rh(N)<sub>2</sub>(CO)<sub>24</sub>]<sup>2-</sup> (2006)

The nitrido-encapsulated heterometallic cluster anions [Co(10)Rh(N)2(CO)21](3-) (1), [Co(10)Rh2(N)2(CO)24](2-) (2), and [Co(11)Rh(N)2(CO)24](2-) (3) have been obtained by tailored redox-condensation reactions. These three anions are rare high-nuclearity mixed-metal clusters containing two interstitial nitrogen atoms. Their structures have been determined by single-crystal X-ray diffraction on their [NR4]+ salts (R = Me for 1 and 3, R = Et for 2), and their electrochemical and ESR properties have been studied in detail. It is noteworthy that 1 has an unprecedented stereochemistry that does not exhibit a close geometrical resemblance with the isoelectronic homometallic anion [Co(11)N2(CO)11(mu2-CO)10](3-), and 2, despite its even number of electrons, is a paramagnetic species.
Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR (2011)

Molecular characterization of CTCs has revealed a remarkable heterogeneity of gene expression between breast cancer patients. In a small percentage of patients, CTCs were positive for all six genes tested, while in some patients only one of these genes was expressed. The clinical significance of these findings in early breast cancer remains to be elucidated when the clinical outcome for these patients is known.
Blockade of A <sub>2A</sub> receptors potently suppresses the metastasis of CD73 <sup>+</sup> tumors (2013)

CD73 inhibits antitumor immunity through the activation of adenosine receptors expressed on multiple immune subsets. CD73 also enhances tumor metastasis, although the nature of the immune subsets and adenosine receptor subtypes involved in this process are largely unknown. In this study, we revealed that A2A/A2B receptor antagonists were effective in reducing the metastasis of tumors expressing CD73 endogenously (4T1.2 breast tumors) and when CD73 was ectopically expressed (B16F10 melanoma). A2A(-/-) mice were strongly protected against tumor metastasis, indicating that host A2A receptors enhanced tumor metastasis. A2A blockade enhanced natural killer (NK) cell maturation and cytotoxic function in vitro, reduced metastasis in a perforin-dependent manner, and enhanced NK cell expression of granzyme B in vivo, strongly suggesting that the antimetastatic effect of A2A blockade was due to enhanced NK cell function. Interestingly, A2B blockade had no effect on NK cell cytotoxicity, indicating that an NK cell-independent mechanism also contributed to the increased metastasis of CD73(+) tumors. Our results thus revealed that CD73 promotes tumor metastasis through multiple mechanisms, including suppression of NK cell function. Furthermore, our data strongly suggest that A2A or A2B antagonists may be useful for the treatment of metastatic disease. Overall, our study has potential therapeutic implications given that A2A/A2B receptor antagonists have already entered clinical trials in other therapeutic settings.
Back-propagation heuristics: a study of the extended delta-bar-delta algorithm (1990)

An investigation is presented of an extension, proposed by A.A. Minai and R.D. Williams (Proc. Int. Joint Conf. on Neural Networks, vol.1, p.676-79, Washington, DC, 1990), to an algorithm for training neural networks in real-valued, continuous approximation domains. Specifically, the most effective aspects of the proposed extension are isolated. It is found that while momentum is particularly useful for the delta-bar-delta algorithm, it cannot be used conveniently because of sensitivity considerations. It is also demonstrated that by using more subtle versions of the algorithm, the advantages of momentum can be retained without any significant drawbacks
<title>Wavelet-based scalable image compression</title> (1995)

In this paper, we present scalable image compression algorithms based on wavelet transform. Recently, the International Standard Organization (ISO) has proposed the JPEG standard for still image compression. JPEG standard not only provides the basic features of compression (baseline algorithm) but also provides the framework of reconstructing images in different picture qualities and sizes. These features are referred to as SNR and spatial scalability, respectively. Spatial scalability can be implemented using the hierarchical mode in the JPEG standard. However, the standard does not specify the downsampling filters to be used for obtaining the progressively lower size images. A straightforward implementation would employ mean downsampling filters. However, this filter does not perform very well in extracting the features from the full size image resulting in poor quality images and a lower compression ratio. We present a wavelet transform based technique for achieving spatial scalability (within the framework of hierarchical mode). Our simulation results confirm the substantial performance improvement and superior subjective quality images using the proposed technique. Most importantly, the wavelet based technique does not require any modifications to existing JPEG decoders.
Experimental Treatment with Favipiravir for COVID-19: An Open-Label Control Study (2020)

There is currently an outbreak of respiratory disease caused by a novel coronavirus. The virus has been named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the disease it causes has been named coronavirus disease 2019 (COVID-19). More than 16% of patients developed acute respiratory distress syndrome, and the fatality ratio was 1%-2%. No specific treatment has been reported. Herein, we examined the effects of favipiravir (FPV) versus lopinavir (LPV)/ritonavir (RTV) for the treatment of COVID-19. Patients with laboratory-confirmed COVID-19 who received oral FPV (Day 1: 1600 mg twice daily; Days 2-14: 600 mg twice daily) plus interferon (IFN)-α by aerosol inhalation (5 million international unit (IU) twice daily) were included in the FPV arm of this study, whereas patients who were treated with LPV/RTV (Days 1-14: 400 mg/100 mg twice daily) plus IFN-α by aerosol inhalation (5 million IU twice daily) were included in the control arm. Changes in chest computed tomography (CT), viral clearance, and drug safety were compared between the two groups. For the 35 patients enrolled in the FPV arm and the 45 patients in the control arm, all baseline characteristics were comparable between the two arms. A shorter viral clearance median time was found for the FPV arm versus the control arm (4 d (interquartile range (IQR): 2.5-9) versus 11 d (IQR: 8-13), <i>P</i> < 0.001). The FPV arm also showed significant improvement in chest CT compared with the control arm, with an improvement rate of 91.43% versus 62.22% (<i>P</i> = 0.004). After adjustment for potential confounders, the FPV arm also showed a significantly higher improvement rate in chest CT. Multivariable Cox regression showed that FPV was independently associated with faster viral clearance. In addition, fewer adverse events were found in the FPV arm than in the control arm. In this open-label before-after controlled study, FPV showed better therapeutic responses on COVID-19 in terms of disease progression and viral clearance. These preliminary clinical results provide useful information of treatments for SARS-CoV-2 infection.
Cytochrome P-450-dependent oxidation of lanosterol in cholesterol biosynthesis. Microsomal electron transport and C-32 demethylation. (1984)

Electron transfer to rat liver microsomal cytochrome P-450 of 14 alpha-methyl group demethylation of 24,25-dihydrolanosterol (C30-sterol) has been studied with a new radio-high-performance liquid chromatography assay. The monooxygenase is dependent upon NADPH plus oxygen, insensitive to CN-, and sensitive to CO. Microsomal oxidation is also sensitive to trypsin digestion, and reactivation is dependent upon the addition of purified, detergent-solubilized cytochrome P-450 reductase. Electron transport of C-32 sterol demethylation can be fully supported by very low concentrations of NADPH (approximately 10 microM) only in the presence of saturating concentrations of NADH (approximately 200 microM) suggesting involvement of cytochrome b5-dependent electron transfer in addition to the NADPH-supported pathway. The cytochrome P-450 of 14 alpha-demethylation has been solubilized with detergents, resolved chromatographically from cytochrome P-450 reductase and cytochrome b5, and fully active C-32 demethylase reconstituted. Incubation of intact microsomes with NADH and very low concentrations of NADPH described above leads to interruption of demethylation without 14 alpha-methyl group elimination. Under these conditions, C-32 oxidation products of the C30-sterol substrate accumulate at the expense of formation of demethylated, C29-sterol products. This enzymic interruption of C-32 demethylation, accumulation of oxygenated C30-sterols, along with subsequent demethylation of the isolated C30-oxysterols under similar oxidative conditions supports the suggestion that 14 alpha-hydroxymethyl and aldehydic sterols are metabolic intermediates of sterol 14 alpha-demethylation. Only very modest inductions of the constitutive cytochrome P-450 isozyme of 14 alpha-methyl sterol oxidase can be obtained with just 2 out of 12 known, potent inducers of mammalian hepatic cytochrome P-450s. Alternatively, administration of complete adjuvant in mineral oil drastically reduces amounts of total microsomal cytochrome P-450 while activity of 14 alpha-methyl sterol oxidase is not affected dramatically. Thus, as much as 2.5-fold enhancement of C-32 oxidase specific activity is obtained when expressed per unit of cytochrome P-450.
Hapalindoles: new alkaloids from the blue-green alga Hapalosiphon fontinalis (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHapalindoles: new alkaloids from the blue-green alga Hapalosiphon fontinalisRichard E. Moore, Chad Cheuk, and Gregory M. L. PattersonCite this: J. Am. Chem. Soc. 1984, 106, 21, 6456–6457Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/ja00333a079https://doi.org/10.1021/ja00333a079research-articleACS PublicationsRequest reuse permissionsArticle Views1111Altmetric-Citations188LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Breakthroughs in Photonics 2014: Phase Change Materials for Photonics (2015)

Materials that undergo an electronic phase change in a reversible manner open up new directions for research in light-matter interactions and photonic devices. The highly tunable dielectric properties, along with the spatial control of insulating and metallic domains, create photonic-crystal-like environments to control light propagation. In this brief overview, recent advances in photonics that utilize solid-state phase-change systems such as vanadium dioxide (VO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) and chalcogenides are discussed. The controllable optical properties such as the refractive index and the optical conductance between two distinct phases in these materials pave the way for switchable photonic devices with memory. Furthermore, the intermediate states with the coexistence of two phases in the vicinity of phase transition in these materials behave as a tunable metamaterial with extraordinary optical properties that is promising for applications such as perfect absorbers.
Changes in the PCB world with new packaging technologies and the integration of passive components into the PCB (1998)

New developments in the semi‐conductor industry lead to higher I/O counts. Packaging is changing to new, smaller packages, like TCPs and CSPs, and the pitch density increases as well. For the fanout of such pages on the PCB, new design rules have to be applied. Blind via holes, sequential build‐up technologies, new ways to form holes, new materials, a lot of questions for the PCB manufacturer. The integration of passive components, such as bypass capacitors and pull up and pull down resistors, into the PCB, go along with the next generation of packaging technology. This adds complexity to the printed circuit boards, leading to a new generation of PCBs that could better be called Integrated Component Boards (ICB). These boards offer a much higher price/area for the PCB manufacturer and at the same time give the OEM better performance with fewer assembly steps and much smaller units.
A Solution-Based Temperature Sensor Using the Organic Compound CuTsPc (2014)

An electrochemical cell using an organic compound, copper (II) phthalocyanine-tetrasulfonic acid tetrasodium salt (CuTsPc,) has been fabricated and investigated as a solution-based temperature sensor. The capacitance and resistance of the ITO/CuTsPc solution/ITO chemical cell has been characterized as a function of temperature in the temperature range of 25-80 °C. A linear response with minimal hysteresis is observed. The fabricated temperature sensor has shown high consistency and sensitive response towards a specific range of temperature values.