gebhart/patrepeval huggingface dataset:
Two‐Dimensional Gel Electrophoresis (1999)

While one-dimensional SDS-PAGE separates proteins on the basis of size, two-dimensional gel electrophoresis separates proteins first on the basis of isoelectric point, then on the basis of size. This method is capable of resolving 1000 to 2000 separate proteins when combined with sensitive detection methods. This unit describes methods for characterizing cell lysates by two-dimensional gel electrophoresis, including modifications for acidic and basic proteins, the use of immobilized pH gradients, and nonreducing/reducing electrophoretic separations. In addition there are support protocols for determining pH profiles of gels, casting Immobiline gels, preparing cell and tissue samples for isoelectric focusing, preparing molecular weight standards, and using two-dimensional protein databases.
Graphene Oxide-Assisted Dispersion of Pristine Multiwalled Carbon Nanotubes in Aqueous Media (2010)

Graphene oxide (GO) sheets, considered as “soft” two-dimensional macromolecules containing multiple aromatic regions and hydrophilic oxygen groups, can adsorb the pristine multiwalled carbon nanotubes (MWNTs) through the π-stacking interaction, thus causing pristine MWNTs to stably disperse and fractionate in aqueous media. The water-soluble and insoluble complexes can be obtained by changing the initial proportion of MWNTs to GO sheets, which are important for noncovalent approaches toward solubilizing CNTs and open a new way for GO applications in colloidal chemistry. Solubility results indicate that the GO sheets are prone to stabilize MWNTs with larger diameters. When the diameters of CNTs decrease to a critical value, the π-stacking interactions are weakened by large surface energy among CNTs themselves; the CNTs are inclined to form bundles or twisted structures instead of CNT−GO complexes. This can be probably developed into a practical method to fractionate MWNTs with different outer diameters in water.
Infrared Assessment of an Optimized Vane Pressure Side Film Cooling Array (2012)

The following experimental work described here entails the investigation by infrared thermography (IRT) of full-scale flat plates intended to model the pressure side (PS) of a modern fully-cooled turbine inlet vane called the High-Impact Technologies (HIT) Research Turbine Vane (RTV). The imaging system is used to make detailed full-coverage, two-dimensional, steady-state measurements of flat plate surface temperature. The PS has a total of 282 film cooling holes including three rows of showerhead holes near the leading edge and a handful of rows downstream depending on the design. The flat plates precisely match the material, thickness, and cooling hole sizes on the RTV, however they are not intended to match the external pressure field or the characteristics of internal cooling beneath the airfoil surface. Surface temperature relative to individual trial freestream gas temperatures is reported for an uncooled plate, a plate with the baseline RTV cooling scheme, and for four different hole types on a plate with a 3D-optimized cooling array designed for the RTV in previous work using genetic algorithms and computational fluid dynamics (CFD). The four different cooling hole shapes tested on the downstream rows of the optimized array plates include cylindrical holes, fan-shaped holes, Vehr holes, and a new cooling hole called a mini-trench shaped (MTS) hole. Experimentation on flat plate models using infrared thermography provides large amounts of valuable data, is inexpensive and highly repeatable relative to large rotating blowdown rigs. The results provide key insights into the differences between full-PS film cooling performance on the plate versus the 3D RTV and suggests to designers the best cooling hole shape for the next build of the RTV which will soon be tested in a full-scale blowdown rig instrumented with heat flux gauges. Overall, results clearly corroborate how cooling was redistributed and improved over the PS of the RTV in the original computational design effort and suggest that certain hole shapes are best suited for certain locations on the flat plate models.
Non-invasive technologies for intracranial pressure/volume measurement (2005)

The paper shows that innovative technologies for noninvasive monitoring, of the cerebral blood flow autoregulation, cerebral blood flow pulse and slow waves also for the registration of the reactions to the neurodiagnostic tests can be developed on the basis of the precise measurement of brain parenchyma acoustic characteristics. The innovative technological equipment for such measurement has been developed. The clinical studies proving the validity of the concepts chosen have been carried out. For the first time the innovative non-invasive method has been designed for the ICP absolute value measurement without the necessity of individual calibration of the system "non-invasive meter - patient".
Three-step approach for wafer sawing lane inspection (2009)

Wafer sawing performance must be closely monitored to ensure a satisfactory integrated circuits manufacturing yield. The inspection must allow the GO/NG decision to be fast and reliable, while also assuring that the training of the inspector is simple and not time consuming. The traditional neural-network approach to inspect images, while simple to implement, presents some disadvantages, including training efficiency and model effectiveness. Based on contour detection of the sawing lane, this work proposes a novel method combined with cross-center localization of sawing lanes, detection of sawing track, and four signatures to detect the abnormality of sawing effectively and timely. Our method does not need pretraining but runs faster and provides a better method with more effectiveness, higher flexibility, and immediate feedback to the sawing operation. An experiment using real data collected from an international semiconductor package factory is conducted to validate the performance of the proposed framework. The accurate acceptance rate and the accurate rejection rate are both 100%, while the false acceptance rate and false rejection rate are both zero as well. The results demonstrate that the proposed method is sound and useful for sawing inspection in industries.
Design of the 3D Input Method Based on Touch Device for Mobile (2009)

This thesis describes the technology to express 3D gesture as an input from common 2-dimension touch input panel without additional hardware development or change to provide near real 3D UI and algorithm to implement such technology. It introduces how 3D user interface has become the major issues among the major mobile vendors and briefly describes existing touch devices which can be divided into resistive and capacitive touch panels. It also introduces Open GLES which takes a major role in graphical presentation of 3D user interface. Algorithm to achieve the 3D gesture in 2D space is presented at last.
Effects of the cold pressor test on cardiac autonomic control in normal subjects (2009)

The cold pressor test (CPT) triggers in healthy subjects a vascular sympathetic activation and an increase in blood pressure. The heart rate (HR) response to this test is less well defined, with a high inter-individual variability. We used traditional spectral analysis together with the non-linear detrended fluctuation analysis to study the autonomic control of HR during a 3-min CPT. 39 healthy young subjects (23.7+/-3.2 years, height 180.4+/-4.7 cm and weight 73.3+/-6.4 kg) were divided into two groups according to their HR responses to CPT. Twenty subjects have a sustained increase in HR throughout the test with reciprocal autonomic interaction, i.e. increase in sympathetic activity and decrease vagal outflow. In the 19 remainders, HR decreased after an initial increase, with indication of involvement of both sympathetic and vagal outflow. Baseline evaluation of the subjects revealed no difference between the two groups. Nevertheless, a higher sympathetic activity at the skin level during CPT was present in the group with decreased HR. Further studies are needed to explain why healthy subjects react differently to the CPT and if this has potential clinical implications.
Connexin 32 dominant-negative mutant transgenic rats are resistant to hepatic damage by chemicals (2004)

Connexins are subunits of gap junction channels, which allow direct transfer of ions, secondary messenger molecules, and other metabolites between contacting cells. Gap junctions are believed to be involved in tissue homeostasis, embryonic development, and control of cell proliferation. Several studies have shown that cell damage signals are transmitted through gap junctions when cells are irradiated or when cells bearing the herpes simplex virus-thymidine kinase (HSV-TK) gene are treated with ganciclovir. We established 2 lines of transgenic rats with a dominant-negative mutant of connexin 32 gene under control of the albumin promoter. In the livers of transgenic rats, membrane localization of normal endogenous connexin 32 protein is disturbed, and gap junction capacity measured by scrape dye-transfer assay in vivo is markedly decreased when compared with wild-type rats. The present investigation concerned susceptibility to the liver-toxic substances D-galactosamine and carbon tetrachloride. These toxicants induced massive liver cell death and elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in the wild-type rats; however, much fewer liver cells were damaged and serum enzyme elevation was much lower in the transgenic rats. In conclusion, gap junctional intercellular communication (GJIC) plays an important role in toxic effects of chemicals; damage or death signals may pass through gap junctions in the rat liver in vivo.
Poststack Velocity Analysis in the Dip-angle Domain Using Diffractions (2008)

In this study a method to analyze diffraction data for migration velocity analysis in the time or depth domain is presented. The method is based on the clear distinction between diffractions and reflections in the post-migration dip-angle domain. The attractive possibility to perform the analysis, using only stacked data as an input, is demonstrated on synthetic and real data examples.
Axenically Grown Amastigotes of <i>Leishmania infantum</i> Used as an In Vitro Model To Investigate the Pentavalent Antimony Mode of Action (1998)

The mechanism(s) of activity of pentavalent antimony [Sb(V)] is poorly understood. In a recent study, we have shown that potassium antimonyl tartrate, a trivalent antimonial [Sb(III)], was substantially more potent than Sb(V) against both promastigotes and axenically grown amastigotes of three Leishmania species, supporting the idea of an in vivo metabolic conversion of Sb(V) into Sb(III). We report that amastigotes of Leishmania infantum cultured under axenic conditions were poorly susceptible to meglumine [Glucantime; an Sb(V)], unlike those growing inside THP-1 cells (50% inhibitory concentrations [IC50s], about 1.8 mg/ml and 22 microg/ml, respectively). In order to define more precisely the mode of action of Sb(V) agents in vivo, we first induced in vitro Sb(III) resistance by direct drug pressure on axenically grown amastigotes of L. infantum. Then we determined the susceptibilities of both extracellular and intracellular chemoresistant amastigotes to the Sb(V)-containing drugs meglumine and sodium stibogluconate plus m-chlorocresol (Pentostam). The chemoresistant amastigotes LdiR2, LdiR10, and LdiR20 were 14, 26, and 32 times more resistant to Sb(III), respectively, than the wild-type one (LdiWT). In accordance with the hypothesis described above, we found that intracellular chemoresistant amastigotes were resistant to meglumine [Sb(V)] in proportion to the initial level of Sb(III)-induced resistance. By contrast, Sb(III)-resistant cells were very susceptible to sodium stibogluconate. This lack of cross-resistance is probably due to the presence in this reagent of m-chlorocresol, which we found to be more toxic than Sb(III) to L. infantum amastigotes (IC50s, of 0.54 and 1.32 microg/ml, respectively). Collectively, these results were consistent with the hypothesis of an intramacrophagic metabolic conversion of Sb(V) into trivalent compounds, which in turn became readily toxic to the Leishmania amastigote stage.
The Child, Before and After (1921)

Journal Article The Child, Before and After Get access THE PRINCIPLES OF ANTE-NATAL AND POST-NATAL PHYSIOLOGY, pure and applied. By W. M. Feldman, asst. physician to and lecturer on child physiology at the Infants' Hospital. With 6 plates and 129 illustrations. Pp. 694. London, Longmans, Green and Co., 1920. P.P. P.P. Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Heredity, Volume 12, Issue 3, March 1921, Page 109, https://doi.org/10.1093/jhered/12.3.109 Published: 01 March 1921
Lithium Isotope Separation on Complex Formation I. Equilibrium Study (1963)

Lithium isotope separation on molecular complex formation has been studied in lithium chloride- 1, 4-dioxane systems. The complex shows an absorption maximum at 270 mμ, and the molar ratio LiCl/dioxane in the complex has been found to be 1:1. The isotope ratio has been determined by a mass spectrometer using a surface ionization ion source. The results show that Li is enriched in the complex and the single stage separation factor is 1.026 in equilibrium at 100 °C. The separation of lithium isotopes seems to occur during the isotope exchange reaction between LiCl-dioxane complex and uncomplexed LiCl.
An overview of the Blu-ray disc application format for HD-movie distribution (2005)

Blu-ray Disc (BD) is the next generation optical disc format, currently being standardized by a large consortium of leading CE, PC, authoring companies and major movie studios. BD offers more than five times the capacity of DVD at 25 GB and 50 GB (dual layer). The BD application format addresses the limitations of DVD-Video by providing a complete user experience. The high storage capacity guarantees the best quality HD video available to the consumer. BD standardizes how content publishers can include executable applications on the disc to realize fully enhanced interactivity. By using a programming platform like Java, the standard need not define the allowable set of features, instead it defines the playback platform and disc publishers are free to implement any features they desire. Furthermore, BD supports seamless integration of Internet content with disc content, allowing synchronized presentation of updated content from the Internet with disc content. With this combination of features, highest quality HD video, enhanced interactivity and Internet connectivity, BD offers consumers a compelling experience. This paper will present an overview of the features that will be supported by the BD application format and the technical solutions used to realize these features.
Information Warfare – what has been happening? (2005)

Information warfare (IW) where countries attacks enemies electronically with computers, is likely to be actively used today, especially by China. IW has been around since the 1990s. Although it was deemed to be the next big thing in computers, the speciality hasn't exploded as expected. Since September 11, these more subtle forms of attack have been overshadowed by the ‘war against terrorism.’ However, there have been may recent cases where information warfare has been used. Many of these cases involved China in one way or the other. Also, earlier this year the UK National Infrastructure Security Coordination Centre (NISCC) issued a warning about a plethora of information warfare attacks from Asia targeting big companies. Information warfare had definitely matured. Indeed it was used by the UK in the recent war on Iraq and many US Government departments carry it out as part of their duties. But, nevertheless, it could suffer from a lack of credibility on the actual battlefield… This article brings to light the level of activity of information warfare today. It is an underground threat that is ongoing between countries. And China is one of the biggest perpetrators.
Probing Internalization Effects and Biocompatibility of Ultrasmall Zirconium Metal-Organic Frameworks UiO-66 NP in U251 Glioblastoma Cancer Cells (2018)

The synthesis of ultrasmall UiO-66 nanoparticles (NPs) with an average size of 25 nm, determined by X-ray powder diffraction and electron microscopies analysis, is reported. The NPs were stabilized in water by dialyzing the NP from the DMF used for the synthesis. DLS measurements confirmed the presence of particles of 100 nm, which are spherical aggregates of smaller particles of 20⁻30 nm size. The NP have a BET surface area of 700 m²/g with an external surface area of 300 m²/g. UiO-66_N (UiO-66 nanoparticles) were loaded with acridine orange as fluorescent probe. UV-vis spectroscopy analysis revealed no acridine loss after 48 h of agitation in simulated body fluid. The biocompatibility of UiO-66_N was evaluated in human glioblastoma (GBM) cell line U251, the most malignant (IV grade of WHO classification) among brain tumors. In U251 cells, UiO-66_N are inert since they do not alter the cell cycle, the viability, migration properties, and the expression of kinases involved in cancer cell growth. The internalization process was evident after a few hours of incubation. After 24 h, UiO-66_N@Acr (UiO-66_N loaded with acridine orange) were detectable around the nuclei of the cells. These data suggest that small UiO-66 are biocompatible NP and could represent a potential carrier for drug delivery in glioblastoma therapies.
Superlog, a unified design language for system-on-chip (2000)

Article Free Access Share on Superlog, a unified design language for system-on-chip Authors: Peter L. Flake Co-Design Automation, Inc., San Jose, CA Co-Design Automation, Inc., San Jose, CAView Profile , Simon J. Davidmann Co-Design Automation, Inc., San Jose, CA Co-Design Automation, Inc., San Jose, CAView Profile Authors Info & Claims ASP-DAC '00: Proceedings of the 2000 Asia and South Pacific Design Automation ConferenceJanuary 2000 Pages 583–586https://doi.org/10.1145/368434.368814Published:28 January 2000Publication History 7citation248DownloadsMetricsTotal Citations7Total Downloads248Last 12 Months58Last 6 weeks9 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Plasmid-directed mechanisms for bacteriophage defense in lactic streptococci (1987)

Genetic studies with lactic streptococci have identified a variety of plasmids coding for systems that interfere with phage adsorption, direct restriction and modification activities, and disrupt various stages in the phage lytic cycle. This review describes mechanisms of phage defense that are plasmid-directed in lactic streptococci, examines the physical and genetic properties of the plasmids involved, and discusses genetic strategies for construction of phage-insensitive starter cultures for dairy fermentations.
Light-Responsive Iron(III)–Polysaccharide Coordination Hydrogels for Controlled Delivery (2015)

Visible-light responsive gels were prepared from two plant-origin polyuronic acids (PUAs), alginate and pectate, coordinated to Fe(III) ions. Comparative quantitative studies of the photochemistry of these systems revealed unexpected differences in the photoreactivity of the materials, depending on the polysaccharide and its composition. The roles that different functional groups play on the photochemistry of these biomolecules were also examined. Mannuronic-rich alginates were more photoreactive than guluronic acid-rich alginate and than pectate. The microstructure of alginates with different mannuronate-to-guluronate ratios changed with polysaccharide composition. This influenced the gel morphology and the photoreactivity. Coordination hydrogel beads were prepared from both Fe-alginate and Fe-pectate. The beads were stable carriers of molecules as diverse as the dye Congo Red, the vitamin folic acid, and the antibiotic chloramphenicol. The photoreactivity of the hydrogel beads mirrored the photoreactivity of the polysaccharides in solution, where beads prepared with alginate released their cargo faster than beads prepared with pectate. These results indicate important structure-function relationships in these systems and create guidelines for the design of biocompatible polysaccharide-based materials where photoreactivity and controlled release can be tuned on the basis of the type of polysaccharide used and the metal coordination environment.
Management of chronic sinusitis in cystic fibrosis (1995)

Chronic rhinosinusitis is extremely common in patients with cystic fibrosis. It causes numerous problems in these patients and can put them at risk for life-threatening illness. Potential problems include nasal obstruction, congestion, sinus pain and pressure, infection (usually with Pseudomonas organisms), hyposmia or anosmia, and the seeding of bacteria into the lower respiratory tract. Cystic fibrosis patients with chronically infected sinuses are at increased risk for pneumonia following lung transplantation. A prophylactic protocol has been developed for the management of chronic sinusitis in patients with cystic fibrosis. These patients are fully evaluated at the Nasal Dysfunction Clinic of the University of California, San Diego (UCSD), Medical Center. Based on the results of the evaluation, they are treated with endoscopic sinus surgery, partial middle turbinectomy, septoplasty, and a large middle meatal maxillary antrostomy. Surgery is followed by a rigorous regimen of pulsatile hypotonic saline nasal irrigation to wash away tenacious cystic secretions. Tobramycin (Nebcin) is given once daily in the nasal irrigant to inhibit the growth of Pseudomonas organisms. At the USCD Nasal Dysfunction Clinic, this prepulmonary transplantation protocol is now used in all cystic fibrosis patients with chronic sinusitis.
Fatty Acids in Eleven Species of Blue-Green Algae: Geochemical Significance (1967)

Analyses of the total lipids of 11 species of blue-green algae showed a simple but qualitatively variable fatty acid composition. The species can be grouped in three categories on the basis of their oleic, linoleic, and linolenic acid content. One species was unusual in that the ten-carbon acid accounts for one-half of its total fatty acid. Branched chain acids are absent in the algae, but are major components of marine bacteria. The geochemical significance of the data is discussed.
Impact of <i>Burkholderia dolosa</i> on Lung Function and Survival in Cystic Fibrosis (2005)

B. dolosa chronic infection in cystic fibrosis is associated with accelerated loss of lung function and decreased survival.