gebhart/patrepeval huggingface dataset:
Influence of the Secondary Cell Wall Polymer on the Reassembly, Recrystallization, and Stability Properties of the S-Layer Protein from <i>Bacillus stearothermophilus</i> PV72/p2 (1998)

The high-molecular-weight secondary cell wall polymer (SCWP) from Bacillus stearothermophilus PV72/p2 is mainly composed of N-acetylglucosamine (GlcNAc) and N-acetylmannosamine (ManNAc) and is involved in anchoring the S-layer protein via its N-terminal region to the rigid cell wall layer. In addition to this binding function, the SCWP was found to inhibit the formation of self-assembly products during dialysis of the guanidine hydrochloride (GHCl)-extracted S-layer protein. The degree of assembly (DA; percent assembled from total S-layer protein) that could be achieved strongly depended on the amount of SCWP added to the GHCl-extracted S-layer protein and decreased from 90 to 10% when the concentration of the SCWP was increased from 10 to 120 microg/mg of S-layer protein. The SCWP kept the S-layer protein in the water-soluble state and favored its recrystallization on solid supports such as poly-L-lysine-coated electron microscopy grids. Derived from the orientation of the base vectors of the oblique S-layer lattice, the subunits had bound with their charge-neutral outer face, leaving the N-terminal region with the polymer binding domain exposed to the ambient environment. From cell wall fragments about half of the S-layer protein could be extracted with 1 M GlcNAc, indicating that the linkage type between the S-layer protein and the SCWP could be related to that of the lectin-polysaccharide type. Interestingly, GlcNAc had an effect on the in vitro self-assembly and recrystallization properties of the S-layer protein that was similar to that of the isolated SCWP. The SCWP generally enhanced the stability of the S-layer protein against endoproteinase Glu-C attack and specifically protected a potential cleavage site in position 138 of the mature S-layer protein.
A new trench isolation technology as a replacement of LOCOS (1984)

A new trench isolation technology that is replaceable to LOCOS and is suitable for submicron VLSIs is presented. The technology is featured with bird's beak free, planar surface, low defect generation, and adaptable to any isolation width from submicron to very large dimensions. The key process steps consist of filling the trench with polysilicon to a half of the trench depth by utilizing photoresist etch back, and subsequent full oxidization of the recessed polysilicon to fill the trench by the oxide. Device characteristics examined experimentally are equivalent to those of LOCCS isolated devices. The feasibility of this technology has been verified successfully by fabricating a 64K bit DRAM.
A functional polymorphism of apolipoprotein C1 detected by mass spectrometry (2006)

A survey of plasma proteins in approximately 1,300 individuals by MALDI-TOF MS resulted in identification of a structural polymorphism of apolipoprotein C1 (ApoC1) that was found only in persons of American Indian or Mexican ancestry. MS/MS analysis revealed that the alteration consisted of a T45S variation. The methyl group of T45 forms part of the lipid-interacting surface of ApoC1. In agreement with an impact on lipid contact, the S45 variant was more susceptible to N-terminal truncation by dipeptidylpeptidase IV in vitro than was the T45 variant. The S45 protein also displayed greater N-terminal truncation (loss of Thr-Pro) in vivo than the T45 variant. The S45 variant also showed preferential distribution to the very-low-density lipoprotein fraction than the T45 protein. These properties indicate a functional effect of the S45 variant and support a role for residue 45 in lipid contact and lipid specificity. Further studies are needed to determine the effects of the variant and its altered N-terminal truncation on the metabolic functions of ApoC1.
Real-time Terascale Implementation of Tele-immersion (2003)

Abstract. Tele-immersion is a new medium that enables a user to share a virtual space with remote participants, by creating the illusion that users at geographically dispersed locations reside at the same physical space. A person is immersed in a remote world, whose 3D representation is acquired remotely, then transmitted and displayed in the viewer’s environment. Tele-immersion is effective only when the three components, computation, transmission, and rendering- all operate in real time. In this paper, we describe the real-time implementation of scene reconstruction on the Terascale Computing System at the Pittsburgh Supercomputing Center.
The influence of ZrO/sub 2/ addition on phase composition in the Nd-Dy-Fe-B system and improved corrosion resistance of the magnets (1994)

The positive influence of ZrO/sub 2/ addition on the magnetic properties, temperature coefficients and corrosion resistance of Nd-Dy-Fe-B magnets were established. The improvements in these parameters were attributed to microstructural changes. The phase composition was analyzed using HREM, and besides the known phases, an additional Zr boride phase was found, which was identified using crystal structure analysis as a ZrB/sub 2/ plate-like phase. Due to the formation of the new phase and oxidation of Nd from the Nd-rich grain boundary phase, the sensitivity of the grain boundaries is diminished to a great extent, and as a consequence the corrosion process does not proceed in the sample. It remains limited to the exposed surface, which is a worthwhile contribution to the improvement of the resistance against corrosive conditions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Affinity-Based Screening of Peptides Recognizing Assembly States of Self-Assembling Peptide Nanomaterials (2009)

Novel peptides capable of binding to self-assembled peptide nanomaterials were identified from a random heptapeptide library displayed on phages. Affinity-dependent peptide screening against helically coiled nanofibers constructed of beta-sheet peptides gave phage clones displaying peptides with a variety of affinities and selectivities. An enzyme-linked immunosorbent assay using phage-displayed peptides revealed that the screened peptides specifically bind to target nanofibers, in contrast to reference nanofibers comprised of peptides with slightly different amino acid sequences. A Dot blot assay using chemically synthesized peptides demonstrated that peptide 01 (p01), with the sequence Thr-Gly-Val-Lys-Gly-Pro-Gly, showed an affinity constant (3.7 x 10(5) M(-1)) for the target nanofibers 200 times greater than its affinity for monomeric peptides and 60 times greater than for short nanofibers. These results suggested that p01 selectively recognizes the assembly state of the target peptide. ATR/IR secondary structure analyses clearly showed that when p01 binds to target nanofibers, it undergoes a structural transition from random-coil to parallel beta-sheet structures, resulting in greater affinity and high specificity for the target fiber. Surface modification of the peptide nanofibers by p01 demonstrated that the peptide specifically binds to the edge of the nanofibers. Using p01, uniform arrays of gold nanoparticles (proteins) could be generated on the peptide nanomaterials.
Angiostatic peptides use plasma fibronectin to home to angiogenic vasculature (2005)

A group of angiogenesis inhibitors are derived from fragments of extracellular matrix or blood proteins. Endostatin, antithrombin, and anastellin are members of this group of substances. The plasma adhesion proteins fibronectin and vitronectin serve as cofactors for these three antiangiogenic proteins. Anginex is a synthetic 33-amino acid peptide that was originally modeled to reproduce the beta-sheet structure of antiangiogenic proteins. Here, we show that anginex initiates fibronectin polymerization and is inactive in mice that lack plasma fibronectin. Anginex shares these characteristics with anastellin. Fluorescein-labeled anginex and anastellin specifically localized in angiogenic vessels in vivo. This localization was dependent on plasma fibronectin and inhibited by an Arg-Gly-Asp peptide. Thus, anginex shares with several physiological angiogenesis inhibitors a dependence on plasma adhesion proteins. The role of the adhesion protein interaction apparently is to form integrin-binding complexes that deliver the antiangiogenic proteins to sites of angiogenesis. This functional convergence of several antiangiogenic factors has important implications for antiangiogenic therapies.
Local Dimensionality Reduction and Supervised Learning Within Natural Clusters for Biomedical Data Analysis (2006)

Inductive learning systems were successfully applied in a number of medical domains. Nevertheless, the effective use of these systems often requires data preprocessing before applying a learning algorithm. This is especially important for multidimensional heterogeneous data presented by a large number of features of different types. Dimensionality reduction (DR) is one commonly applied approach. The goal of this paper is to study the impact of natural clustering--clustering according to expert domain knowledge--on DR for supervised learning (SL) in the area of antibiotic resistance. We compare several data-mining strategies that apply DR by means of feature extraction or feature selection with subsequent SL on microbiological data. The results of our study show that local DR within natural clusters may result in better representation for SL in comparison with the global DR on the whole data.
Visual Analytics of Stratigraphic Correlation for Multi-Attribute Well-Logging Data Exploration (2019)

Stratigraphic correlation based on well-logging data is able to help geological interpreters analogize and deduce underground sedimentary morphology. A great deal of traditional methods has been studied to automatically or manually achieve stratigraphic correlation, the courses of which are usually time-consuming and not intuitive. Many uncertainties are easily generated to reduce the effectiveness of stratigraphic correlation and the accuracy of geological interpretation. To address this issue, this paper introduces an interactive visual analytics system for identifying correlation patterns and improving correlation accuracies using large-scale well-logging data. First, we propose a novel stratigraphic correlation model with the composition of multi-log curve integration, layer identification, and layer matching. Then, a visualization framework is designed by working closely with domain experts in an iterative manner to get deeper insights into the course of stratigraphic correlation based on a few visual interfaces such as map view, correlation view, matrix view and attribute view. Also, a rich set of interactions is provided allowing interpreters to refine the results of stratigraphic correlation according to domain knowledge and user requirements. Furthermore, case studies based on real-world datasets and interviews with domain experts have demonstrated the effectiveness of our system for the stratigraphic correlation and geological interpretation.
Analysis of the Mechanic Behavior and Rutting of Asphalt Concrete Using a Sand Model (1999)

Abstract The stress-strain relationship of the sand and asphalt concrete materials is one of the most important research subjects in the past, and many conctitutive laws for these materials have been proposed in the last two decades. In this study, the Vermeer plasticity model is modified and used to predict the behavior of the sand and asphalt concrete materials under different stress path conditions. The results show that the predictions and test results agree well under different stress path conditions. However, the orignal Vermeer model can not predict the stress-strain behavior of the asphalt concrete. Finally, the modified Vermeer plasticity model is incorporated with the pavement rutting model to predict the rut depth of pavement structure under traffic loadings.
Sequential trisomization of chromosomes 6 and 7 in mouse skin premalignant lesions (1989)

Using a direct cytogenetic technique, we identified a nonrandom trisomy of chromosome 6 in 12 of 12 aneuploid mouse skin papillomas and in 10 of 11 squamous cell carcinomas induced by chemical carcinogenesis. The second most common abnormality observed was trisomy of chromosome 7 found in most dysplastic papillomas and in 10 of 11 carcinomas. The two trisomies were the only abnormalities found in all aneuploid papillomas and in several carcinomas. Mutation at codon 61 of the Ha-ras gene, which resides on chromosome 7, was also a common feature of the tumors sampled. Extensive homology exists between mouse chromosome 6 and human chromosome 7, the trisomy of which was recently suggested as a primary cytogenetic event in several human epithelial cancers. We propose a multistep model of tumor progression in which a sequence of specific nonrandom chromosomal abnormalities appear to be required for malignant transformation.
A Novel Paradigm of Fatty Acid β-Oxidation Exemplified by the Thioesterase-Dependent Partial Degradation of Conjugated Linoleic Acid That Fully Supports Growth of <i>Escherichia coli</i> (2008)

An alternative pathway of beta-oxidation for unsaturated fatty acids was studied in Escherichia coli. 9- cis,11- trans-Octadecadienoic acid (conjugated linoleic acid), a potential substrate of this pathway, was shown to support growth of E. coli in the absence of any other carbon source. The identification of 3,5-dodecadienoic acid in the growth medium revealed the partial beta-oxidation of conjugated linoleic acid to 3,5-dodecadienoyl-CoA, which was hydrolyzed to 3,5-dodecadienoic acid and released from cells. The involvement of acyl-CoA thioesterases in this process was evaluated by determining the substrate specificity of thioesterase II and comparing it with that of a novel thioesterase (thioesterase III) and by assessing mutant strains devoid of one or both of these thioesterases for growth on conjugated linoleic acid. Both thioesterases were highly active with 3,5-dodecadienoyl-CoA as substrate. A deficiency of either thioesterase decreased the growth rate of cells on conjugated linoleic acid but not on palmitic acid. The absence of both thioesterases reduced the cellular growth in a cumulative manner but did not abolish it. It is concluded that thioesterases II and III and at least one other thioesterase function in the partial degradation of conjugated linoleic acid via the thioesterase-dependent pathway of beta-oxidation, which provides all energy and carbon precursors required for the growth of E. coli.
UML Modeling of Network Topologies for Distributed Computer System (2009)

Nowadays distributed computer systems have become very popular approach due to its availability at low cost and high performance computers, which are connected through a communication network. For connection of the distributed computer systems, network topologies are must for the communication lines. In the present paper a detailed study of network topologies is done for the distributed computer systems. A most popular Unified Modeling Language (UML) is used for designing the different network topologies. A comparative study is done for 2D Mesh, Torus, and Hypercube topologies and performance is evaluated after designing the UML Class, Sequence, and Activity diagrams for these topologies.
Advanced Well Control using Rapid Cross-linking Polymers (2017)

Abstract Industry maintains well control through proper well design and appropriate controls and barriers. This has made loss of well control a very low probability event. Currently the final barrier to maintain control is a valve system (blowout preventer or BOP) located on top of wells capable of sealing around or shearing through obstructions that might be in the well (e.g. drilling pipe and casing) to isolate the well. Although the risk is low when proper drilling practices and design are employed, there are still concerns about well control especially for operations in sensitive environments. Adding an additional barrier could alleviate these concerns. One scenario for well control loss is if the BOP fails to seal allowing drilling fluids and reservoir fluids to flow. We are currently evaluating a concept to respond to such an event and seal leaking BOPs by injecting a liquid monomer and a catalyst below a BOP leak point to form a polymer-plug seal. Mixtures of dicyclopentadiene (DCPD) and other monomers mixed with a ruthenium-based catalyst cause a rapid polymerization reaction that forms a stable solid. These reactions can occur under extreme temperatures and pressures and withstand significant contamination from other fluids and solids. Lab studies have shown that DCPD-based polymer plugs can withstand axial stress of 15,000 psi without significant deformation even at temperatures of 200°C and with 20% drilling fluid contamination. For well control, one option is to preposition monomer mixes and catalyst into pressurized cannisters located at or near subsea BOPs while drilling high-complexity wells. Connecting the pressurized cannisters to appropriate ports on the BOP will allow rapid transfer. During a well-control event, actuating valves would rapidly force the monomer mixes and catalyst from the cannisters into the BOP to initiate polymerization. Polymerization reactions can be as short as a few seconds depending on the monomer mix and catalyst. The resulting solid polymer plug will block the leak path to potentially seal the well. This paper describes the concept details and summarizes the current status of research.
Assessment of disease severity in parkinsonism with fluorine-18-fluorodeoxyglucose and PET. (1995)

These findings indicate that regional metabolic covariance patterns are robust imaging markers of disease severity. FDG-PET may be useful clinically in assessing parkinsonian disability and disease progression.
Slip-rolling resistance of novel Zr(C,N) thin film coatings under high Hertzian contact pressures (2010)

Heutzutage sind Beschichtungen in breiten technologischen Anwendungsfeldern von Dekorierungszwecken bis zur Verbesserung der Leistung von Werkzeugen in der Massenfertigung, von medizinischen Werkzeugen oder noch von Computer Bestandteilen und vielen Anderen verbreitet. Besonders rechnet die Automobilindustrie mit einem Leistungsgewinn bei der Verwendung von Dünnschichten in mechanischen Bauteilen des Antriebstranges und des Getriebes. Das auf einer kostengünstigen Alternative basierte Konzept ist die Leistungssteigerung durch Erhöhung der Tragfähigkeit durch Aufbringung von Dünnschichten, die zu zusätzlichen Eigenschaften beitragen könnten, ohne das Design der Bauteile grundlegend zu verändern. Es würde auch einen Weg in Richtung Downsizing darstellen. In der vorliegenden Arbeit ist eine kurz gefasste Literaturübersicht bezüglich der Wälzbeständigkeit von verschiedenen Dünnschichten zusammengestellt. Bei der Durchführung von Wälzversuchen sind kristalline Zr(C,N) Dünnschichten als wälzbeständig nachgewiesen worden und zwar bis zu einer mittleren Hertzschen Kontaktpressung von P0mitteln = 1,94 GPa (P0max = 2,91 GPa) bei 120°C in Erstbefüllungsmotoröl bis zu zehn Millionen Zyklen. Grundsätzlich stellt dieses Ergebnis hier eine Verdoppelung der auf die Oberfläche wirkenden Normalkraft gegenüber unbeschichteter Kontaktkonfiguration dar, die herkömmlich mit gebrauchsfertig formulierten Ölen (d.h. mit hohem Anteil an Additiven) geschmiert sind. Typischerweise bestehen die zu beschichtenden Substrate aus vergütetem Lagerstahl Cronidur 30. Die Zr(C,N) Dünnschichten sind mittels verschiedenen Untersuchungsmethoden charakterisiert worden, um die Ursachen festgestellter Ergebnisunterschiede bezüglich der Wälzbeständigkeit unter diesen hohen tribologischen Beanspruchungen zu klären. Die Wälzbeständigkeit verschiedener Beschichtungschargen ist mittels eines festgelegten, leistungsfähigen Prüfverfahrens evaluiert worden. Verschiedene Standzeitergebnisse sind zwischen den einzelnen Chargen erreicht worden, ohne wesentliche Änderungen bei den Abscheidungsprozessen vorzunehmen. Die durchgeführte Charakterisierung stellt mikrostrukturelle Unterschiede fest, die die Wälzbeständigkeit beeinflussen und als Ursache der Wälzverhaltensunterschiede der Zr(C,N) Dünnschichten sein können. Außerdem ist die Leistung der Zr(C,N) Dünnschicht nicht nur im Bezug auf die Steigerung der Wälzbeständigkeit sondern auch hinsichtlich des tribologischen Einflusses (u. a. Nachwirkungen auf den Verschleiß und die Reibung) bewertet worden. Darum wurden die tribologischen Ergebnisse mit den entsprechenden gemessenen Größen von unlängst entwickelten DLC-Dünnschichten (DLC, Diamond Like Carbon) verglichen, die auch bei der gleichen Prüfprozedur getestet worden sind und gleiche Überrollungszahlen erwiesen hatten.
Volume holographic lenses and their applications in white light imaging and concentration of solar energy (2002)

In this paper we present the design and development of volume holographic lenses for white light imaging and solar energy concentration. Details of design analysis and process of manufacturing of these lenses on dichromated gelatin is also given.
Synthesis of 2-substituted 5-acetyl-1(H)-imidazoles via 3-chloro-4,4-dimethoxy-2-butanone and related 3,4-disubstituted 3-buten-2-ones (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 2-substituted 5-acetyl-1(H)-imidazoles via 3-chloro-4,4-dimethoxy-2-butanone and related 3,4-disubstituted 3-buten-2-onesLawrence A. ReiterCite this: J. Org. Chem. 1984, 49, 19, 3494–3498Publication Date (Print):September 1, 1984Publication History Published online1 May 2002Published inissue 1 September 1984https://pubs.acs.org/doi/10.1021/jo00193a012https://doi.org/10.1021/jo00193a012research-articleACS PublicationsRequest reuse permissionsArticle Views626Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Study of Habitat Preferences in the Grey Kangaroo (1981)

In a state forestry reserve in south-eastern Queensland, indices of grey kangaroo abundance were obtained from faecal pellet counts and were related to habitat structure by analysis of variance, multiple regression and cluster analysis. Of these, cluster analysis proved the most effective technique. Results identified a preference for habitats displaying an abundance of food reserves and shelter. Climax forest and open woodland type habitats were relatively little used.
60 YEARS AGO (2001)

AbstractWith my look back a year ago I tried to generate some correspondence but not a single letter. I am hoping for better luck this time in getting a lively letters page back in the journal.