gebhart/patrepeval huggingface dataset:
Three Restriction Endonucleases from Anabaena flos-aquae (1985)

Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be: (formula; see text) AflII and AflIII are new specificities and may be useful in molecular cloning, as well as in the analysis of DNA. The distribution of type II restriction endonucleases in the cyanobacteria is briefly discussed.
Visuo-Spatial Working Memory Span: A Sensitive Measure of Cognitive Deficits in Children With ADHD (2004)

Working memory (WM) has been hypothesised to be impaired in attention-deficit/hyperactivity disorder (ADHD). However, there are few studies reported on tests measuring visuo-spatial WM (VSWM) in ADHD. Some of these studies used paradigms including episodic memory, others only used low memory loads. In the present study we used a VSWM test that has not been used previously in ADHD research. The sensitivity of the VSWM test and a choice reaction time (CRT) test was evaluated in a pilot study by comparing them to two commonly used tests in ADHD-research; the Continuous Performance Test (CPT) and a Go/no-go test, in children with and without ADHD. The groups differed significantly in performance on the VSWM test (P < .01) and CRT (P < .05) but not on the CPT (P > .1) or on the Go/no-go test (P > .1). The results from the VSWM and CRT tests were replicated in a larger sample of participants (80 boys; 27 boys with ADHD and 53 controls, mean age 11.4 years). The difference between the groups was significant for both the VSWM test (P < .01) and the CRT test (P < .01). The effect size (ES) of the VSWM test was 1.34. There was a significant age-by-group interaction on the VSWM test, with larger group differences for the older children (P < .01). Our results show that the VSWM test is a sensitive measure of cognitive deficits in ADHD and it supports the hypothesis that deficits in VSWM is a major component of ADHD.
Clinical use of CT1341 anaesthetic ('Saffan') in marmosets (<i>Callithrix jacchus</i>) (1975)

An intramuscular dose of 18 mg/kg bodyweight of this steroid anaesthetic produced surgical anaesthesia with rapid induction, safe, efficient maintenance and rapid recovery without side effects.
Correction of ornithine accumulation prevents retinal degeneration in a mouse model of gyrate atrophy of the choroid and retina (2000)

Deficiency of ornithine-delta-aminotransferase (OAT) in humans results in gyrate atrophy of the choroid and retina (GA), an autosomal recessive disorder characterized by ornithine accumulation and a progressive chorioretinal degeneration of unknown pathogenesis. To determine whether chronic, systemic reduction of ornithine can prevent this form of retinal degeneration, we used an arginine-restricted diet to maintain long term reduction of ornithine in a mouse model of OAT-deficiency (Oat(-/-)) produced by gene targeting. We evaluated the mice over a 12-month period by measurement of plasma amino acids, electroretinograms, and retinal histologic and ultrastructural studies. We found that an arginine-restricted diet substantially reduces plasma ornithine levels and completely prevents retinal degeneration in Oat(-/-). This result indicates that ornithine accumulation is a necessary factor in the pathophysiology of the retinal degeneration in GA and that restoration of OAT activity in retina is not required for effective treatment of GA.
High voltage charge pump using standard CMOS technology (2004)

An integrated high voltage charge pump circuit utilising intrinsic process features is introduced. It can produce +20 V to +50 V output from a typical 5 V input. The reported charge pumps achieved the highest density and highest output voltages of the industry. Measurements show output ripples of 400 mV for frequencies around 10 MHz and output load of 28 pF. The reported integrated high voltage charge pump circuits was implemented on 0.8 /spl mu/m DALSA semiconductor technology using standard CMOS devices.
Low-Dose-Rate Radiation Attenuates the Response of the Tumor Suppressor TP53 (1999)

Acute low-dose irradiation (0.1-1 Gy, 1.33 Gy/min) of cells of a human glioblastoma cell line, A-172, induced a dose-dependent monophasic accumulation of TP53 (formerly known as p53) and CDKN1A (formerly known as WAF1). In contrast, chronic gamma irradiation (0.001 Gy/min) produced a clear biphasic response of accumulation TP53 with the first peak at 1.5 h (0.09 Gy) and the second peak at 10 h (0.54 Gy). Significantly, when the cells were preirradiated with a chronic dose of gamma irradiation for 24 h (1.44 Gy) or 50 h (3 Gy), they no longer responded to an acute challenging dose to produce a dose-dependent response of the TP53 pathway. These findings suggest that chronic irradiation at low dose rate alters the TP53-dependent signal transduction pathway. Wearing away of the TP53 pathway by chronic exposure to radiation may have important implications for radiation protection.
Triangulation-based 3D surveying borescope (2016)

In this work, a measurement concept based on triangulation was developed for borescopic 3D-surveying of surface defects. The integration of such measurement system into a borescope environment requires excellent space utilization. The triangulation angle, the projected pattern, the numerical apertures of the optical system, and the viewing angle were calculated using partial coherence imaging and geometric optical raytracing methods. Additionally, optical aberrations and defocus were considered by the integration of Zernike polynomial coefficients. The measurement system is able to measure objects with a size of 50 μm in all dimensions with an accuracy of ± 5 μm. To manage the issue of a low depth of field while using an optical high resolution system, a wavelength dependent aperture was integrated. Thereby, we are able to control depth of field and resolution of the optical system and can use the borescope in measurement mode with high resolution and low depth of field or in inspection mode with low resolution and higher depth of field. First measurements of a demonstrator system are in good agreement with our simulations.
Ventilatory response to exercise in subjects breathing CO<sub>2</sub> or HeO<sub>2</sub> (1997)

To investigate the effects of mechanical ventilatory limitation on the ventilatory response to exercise, eight older subjects with normal lung function were studied. Each subject performed graded cycle ergometry to exhaustion once while breathing room air; once while breathing 3% CO2-21% O2-balance N2; and once while breathing HeO2 (79% He and 21% O2). Minute ventilation (VE) and respiratory mechanics were measured continuously during each 1-min increment in work rate (10 or 20 W). Data were analyzed at rest, at ventilatory threshold (VTh), and at maximal exercise. When the subjects were breathing 3% CO2, there was an increase (P < 0.001) in VE at rest and at VTh but not during maximal exercise. When the subjects were breathing HeO2, VE was increased (P < 0.05) only during maximal exercise (24 +/- 11%). The ventilatory response to exercise below VTh was greater only when the subjects were breathing 3% CO2 (P < 0.05). Above VTh, the ventilatory response when the subjects were breathing HeO2 was greater than when breathing 3% CO2 (P < 0.01). Flow limitation, as percent of tidal volume, during maximal exercise was greater (P < 0.01) when the subjects were breathing CO2 (22 +/- 12%) than when breathing room air (12 +/- 9%) or when breathing HeO2 (10 +/- 7%) (n = 7). End-expiratory lung volume during maximal exercise was lower when the subjects were breathing HeO2 than when breathing room air or when breathing CO2 (P < 0.01). These data indicate that older subjects have little reserve for accommodating an increase in ventilatory demand and suggest that mechanical ventilatory constraints influence both the magnitude of VE during maximal exercise and the regulation of VE and respiratory mechanics during heavy-to-maximal exercise.
Silicon nanowire optical waveguide (SNOW) (2010)

In this paper, we propose a novel optical waveguide consisting of arrays of silicon nanowires in close proximity. We show that such a structure can guide an optical mode provided the electric field is polarized along the length of the nanowires. Furthermore, such guidance can happen even if the nanowires are arranged randomly albeit at a higher scattering loss. On the other hand, high radiation losses are observed if the electric field is polarized in the transverse direction to the nanowires. We calculate the optical radiation loss for different structures using Finite Difference Time Domain (FDTD) method. We also show that the arrayed nanowire region can be approximated using an effective index bulk waveguide. The approximation allows for design and optimization of optical structures using integrated optics methodology resulting in significant savings in time and resources. The advantage of the proposed waveguide structure is that it allows for increased optical confinement while using the enhanced optical interactions of nanowire structures compared to single nanowire photonic waveguide for diameters smaller than 100 nm. For a diameter of 50 nm for the silicon nanowire, an optical confinement factor of 33 % was achieved in the proposed waveguide as opposed to 0.1 % that is achieved for a single nanowire photonic waveguide. A radiation loss of 0.12 cm(-1) is achieved for nanowires of the same diameter spaced 75 nm apart. While our analysis is done on silicon nanowires at 1550 nm, the proposed structures can be extended to other materials and wavelength regimes also.
CRC Standard Mathematical Tables and Formulae (2011)

With over 6,000 entries, CRC Standard Mathematical Tables and Formulae, 32nd Edition continues to provide essential formulas, tables, figures, and descriptions, including many diagrams, group tables, and integrals not available online. This new edition incorporates important topics that are unfamiliar to some readers, such as visual proofs and sequ
Seeded emulsion polymerization of <i>n</i>‐butyl acrylate utilizing miniemulsions (1991)

Abstract Nucleation of polymer particles in the seeded emulsion polymerization of n ‐butyl acrylate (BuA) was studied through experiments designed to control the amount of new particles formed. The results show that for the batch and semicontinuous seeded polymerization of BuA, a small amount of new particles was formed in the system in which the monomer was added neat, whereas a singificant amount of new particles was formed when the monomer was added as a miniemulsion. This suggests that new particles formed in the miniemulsion process were from nucleation of the monomer droplets. These experiments also showed that monomer‐droplet nucleation decreased with increasing seed concentration in the reactor. For the seeded semicontinuous polymerizations, monomer‐droplet nucleation decreases with decreasing BuA miniemulsion feed rate. The results also show that monomerdroplet nucleation takes place whenever miniemulsion droplets exist in the reactor. This study suggests that miniemulsions can be used to control the particle size distribution of a polymer latex system.
Reactive ion etch studies of DUV resists (1999)

The morphology and degradation of the polymeric DUV resist of polyhydroxystyrene/t-butyl acrylate copolymers after CF<SUB>4</SUB>/O<SUB>2</SUB>/Ar/CHF<SUB>3</SUB> reactive ion etching (RIE) was studied. The surface pitting in the area of 10 nm with spikes as large as 85 nm were found. DUV resists with higher t-butylacrylate content in the main chain were susceptible to main chain scission and surface pitting. The entrapment of volatile fragments by the deposited amorphous fluorocarbon polymer during the RIE process leads to surface distortions. Pre-hardening of the resist by electron beam reduces the surface pitting by two fold. Negative resist based on polyhydroxystyrene homopolymer with minimum volatile produced very smooth films with a tenfold reduction in roughness factor. The future design of positive resist should include the RIE performance factor of minimal outgassing and minimum sidewall surface roughness.
Both the N-terminal Loop and Wing W2 of the Forkhead Domain of Transcription Factor Foxo4 Are Important for (2007)

FoxO4 belongs to the “O” subset of forkhead transcription factors, which participate in various cellular processes. The forkhead DNA binding domain (DBD) consists of three-helix bundle resting on a small antiparallel -sheet from which two extended loops protrude and create two wing-like structures. The wing W2 of FoxO factors contains a 14-3-3 protein-binding motif that is phosphorylated by protein kinase B in response to insulin or growth factors. In this report, we investigated the role of the N-terminal loop (portion located upstream of first helix H1) and the C-terminal region (loop known as wing W2) of the forkhead domain of transcription factor FoxO4 in DNA binding. Although the deletion of either portion partly reduces the FoxO4-DBD binding to the DNA, the simultaneous deletion of
Stable Load-Carrying and Rotational Loss Characteristics of Diamagnetic Bearings (2004)

We investigated the use of diamagnetic materials in magnetic bearings using an experimental apparatus to study the static equilibrium, load-carrying capacity, and dynamic characteristics of the bearings. The apparatus consisted of a 0.79-g NdFeB disk-shaped permanent-magnet rotor levitated by a ferrite magnet with a diamagnetic stabilizer made of either bismuth or graphite, all in a bell-jar vacuum chamber to eliminate air friction on the rotor. The equilibrium position of the rotor was statically and dynamically stable, according to theoretical calculations based on the magnetic-image method. In dynamic tests, the rotor of the diamagnetic bearing was spun up to 100 Hz by a tangential jet of nitrogen gas and then was allowed to spin down freely to measure the rotational losses. The rotational losses varied with the frequency of the rotation, indicating that most of the losses were mainly due to eddy currents. Minor losses due to magnetic inhomogeneities in the permanent magnets and diamagnetic parts were also observed over a small frequency range.
Rapid methods for concurrent measurement of the RF‐pulse flip angle and the longitudinal relaxation time (2009)

Measuring both the flip angle (FA) and the longitudinal relaxation time T(1) is essential in quantitative and longitudinal studies because the signal amplitude is dependent on these quantities. Conventional methods can only measure one of them at a time and require long scan times. In this work, two mutually consistent methods are developed; each can acquire multislice data for determining both the FA and T(1) in a scan time about half the time needed for a conventional FA measurement. On the basis of a recent development of longitudinal-relaxation measurement (Hsu and Lowe, J Magn Reson 2004;169:270-278; Hsu and Glover, J Magn Reson 2006;181:98-106), one of the methods uses RF pulse trains of two FAs whereas the other uses pulse trains of different pulse spacing. When only the FA or T(1) is needed, the present methods can still be faster than conventional methods for the needed quantity. In benchmarking with a uniform-density sample, both methods generate precise T(1) values independent of the FA chosen (except at and near 90 degrees ). In the demonstration with three normal volunteers at 3 T, the T(1) values of frontal and occipital white matter, putamen, and caudate are compared; the T(1) values are in agreement with literature values and the intrasubject deviation is 0.2%-2.8%.
Determination of Serotypes of Shiga Toxin-Producing<i>Escherichia coli</i>Isolates by Intact Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (2010)

Shiga toxin-producing Escherichia coli (STEC) isolates representing the serotypes O165:H25, O26:H11/H32, and O156:H25 were analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectra of whole cells, a procedure also known as intact cell mass spectrometry (ICMS or IC-MALDI MS) or MALDI-typing. We demonstrate that within the given species the three serotypes can be well discriminated by ICMS. Conditions for the construction of serotype-specific prototypic mass spectra were systematically optimized by filtering out masses that do not contribute to the discrimination of the serotypes. Binary distances between prototypic spectra and sample spectra were used to determine serotypes of unknown samples. With parameters optimized, only 0.7% of the assignments were incorrect compared to 31% when distances were calculated from alignments of unfiltered mass spectra. Within the different serotypes, clusters of genetically related E. coli most probably originating from single clones could be distinguished by restriction fragment length polymorphism analysis. Since ICMS did not reproduce these clusters, we conclude that the power of ICMS is just sufficient to discriminate E. coli serotypes under certain conditions but fails for the differentiation of E. coli below this level.
A comparison of range-Doppler and wavenumber domain SAR focusing algorithms (1992)

Focusing of SAR data requires a space-variant two-dimensional correlation. Different algorithms are compared with each other in terms of their focusing quality and their ability to handle the space-variance of the correlation kernel: the range-Doppler approach with and without secondary range compression, modified range-Doppler algorithms, and four versions of the wavenumber domain processor. The phase aberrations of the different algorithms are given in analytic form. Numerical examples are presented for Seasat and ERS-1. A novel systems theoretical derivation of the wavenumber domain algorithm is presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Acoustic vector-sensor correlations in ambient noise (2001)

Most array-processing methods require knowledge of the correlation structure of the noise. While such information may sometimes be obtained from measurements made when no sources are present, this may not always be possible. Furthermore, measurements made in-situ can hardly be used to analyze system performance before deployment. The development of models of the correlation structure under various environmental assumptions is therefore very important. In this paper, we obtain integral and closed form expressions for the auto- and cross-correlations between the components of an acoustic vector sensor (AVS) for a wideband-noise field, under the following assumptions concerning its spatial distribution: 1) azimuthal independence; 2) azimuthal independence and elevational symmetry; and 3) spherical isotropy. We also derive expressions for the cross-covariances between all components of two spatially displaced AVSs in a narrowband-noise field under the same assumptions. These results can be used to determine the noise-covariance matrix of an array of acoustic vector sensors in ambient noise. We apply them to a uniform linear AVS array to asses its beamforming capabilities and localization accuracy, via the Cramer-Rao bound, in isotropic and anisotropic noise.
Low-level image processing and edge enhancement using a self-organizing neural network (1990)

A self-organizing artificial neural network has been described to enhance and restore gray-level images for applications in low-level image processing. The image is described by a set of interconnected neurons with their values equal to the gray-level values of corresponding pixels. The first-order and second-order contrast links are defined among the neurons which are analyzed for a change in their values in the adaptive constrained environment. Each selected neuron is analyzed only once per iteration, in which its value may be readjusted by incrementing or decrementing the current value. As a result, at the end of each iteration the image data is reorganized. The structure and algorithm of the proposed neural network are presented along with various experimental results showing the capability of such a network to restore and enhance the gray-level images
PHOG, a candidate gene for involvement in the short stature of Turner syndrome (1997)

The abnormalities seen in Turner syndrome (monosomy X) presumably result from haploinsufficiency of certain genes on the X chromosome. Gene dosage considerations lead to the prediction that the culpable genes escape X inactivation and have functional homologs on the Y chromosome. Among the genes with these characteristics are those residing in the pseudoautosomal regions (PAR) of the sex chromosomes. A pseudoautosomal location for a dosage-sensitive locus involved in stature has been suggested based on the analyses of patients with deletions of a specific segment of the short arm PAR; hemizygosity for this putative locus probably also contributes to the short stature in Turner individuals. We have isolated a gene from the critical deleted region that encodes a novel homeodomain-containing transcription factor and is expressed at highest levels in osteogenic cells. We have named the gene PHOG, for pseudoautosomal homeobox-containing osteogenic gene. Its deletion in patients with short stature, the predicted altered dosage in 45,X individuals, along with the nature of the encoded protein and its expression pattern, make PHOG an attractive candidate for involvement in the short stature of Turner syndrome. We have also found that the mouse homolog of PHOG is autosomal, which may help to explain the lack of a growth abnormality in mice with monosomy X.
<i>α</i> 11β1 Integrin-Dependent Regulation of Periodontal Ligament Function in the Erupting Mouse Incisor (2007)

The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated a null allele of the alpha11 gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective incisors. Mutant incisors are characterized by disorganized periodontal ligaments, whereas molar ligaments appear normal. The primary defect in the incisor ligament leads to halted tooth eruption. alpha11beta1-defective embryonic fibroblasts displayed severe defects in vitro, characterized by (i) greatly reduced cell adhesion and spreading on collagen I, (ii) reduced ability to retract collagen lattices, and (iii) reduced cell proliferation. Analysis of matrix metalloproteinase in vitro and in vivo revealed disturbed MMP13 and MMP14 synthesis in alpha11(-/-) cells. We show that alpha11beta1 is the major receptor for collagen I on mouse embryonic fibroblasts and suggest that alpha11beta1 integrin is specifically required on periodontal ligament fibroblasts for cell migration and collagen reorganization to help generate the forces needed for axial tooth movement. Our data show a unique role for alpha11beta1 integrin during tooth eruption.