gebhart/patrepeval huggingface dataset:
Differential Temperature-dependent Chaperone-like Activity of αA- and αB-crystallin Homoaggregates (1999)

alpha-Crystallin, a heteromultimeric protein made up of alphaA- and alphaB-crystallins, functions as a molecular chaperone in preventing the aggregation of proteins. We have shown earlier that structural perturbation of alpha-crystallin can enhance its chaperone-like activity severalfold. The two subunits of alpha-crystallin have extensive sequence homology and individually display chaperone-like activity. We have investigated the chaperone-like activity of alphaA- and alphaB-crystallin homoaggregates against thermal and nonthermal modes of aggregation. We find that, against a nonthermal mode of aggregation, alphaB-crystallin shows significant protective ability even at subphysiological temperatures, at which alphaA-crystallin or heteromultimeric alpha-crystallin exhibit very little chaperone-like activity. Interestingly, differences in the protective ability of these homoaggregates against the thermal aggregation of beta(L)-crystallin is negligible. To investigate this differential behavior, we have monitored the temperature-dependent structural changes in both the proteins using fluorescence and circular dichroism spectroscopy. Intrinsic tryptophan fluorescence quench-ing by acrylamide shows that the tryptophans in alphaB-crystallin are more accessible than the lone tryptophan in alphaA-crystallin even at 25 degrees C. Protein-bound 8-anilinonaphthalene-1-sulfonate fluorescence demonstrates the higher solvent accessibility of hydrophobic surfaces on alphaB-crystallin. Circular dichroism studies show some tertiary structural changes in alphaA-crystallin above 50 degrees C. alphaB-crystallin, on the other hand, shows significant alteration of tertiary structure by 45 degrees C. Our study demonstrates that despite a high degree of sequence homology and their generally accepted structural similarity, alphaB-crystallin is much more sensitive to temperature-dependent structural perturbation than alphaA- or alpha-crystallin and shows differences in its chaperone-like properties. These differences appear to be relevant to temperature-dependent enhancement of chaperone-like activity of alpha-crystallin and indicate different roles for the two proteins both in alpha-crystallin heteroaggregate and as separate proteins under stress conditions.
Neutrophil Elastase Degrades Cystic Fibrosis Transmembrane Conductance Regulator via Calpains and Disables Channel Function <i>In Vitro</i> and <i>In Vivo</i> (2012)

These data provide a new mechanism and show for the first time a link between NE-calpains activation and CFTR loss of function in bacterial lung infections relevant to CF and to other chronic inflammatory lung conditions.
Synergism of Cytotoxic Effects of Vinorelbine and Paclitaxel in Vitro (2000)

Empiric combinations of vinca alkaloids with taxanes have been recently used in clinical oncology. To enhance the activity of these two classes of agents, we evaluated the sequence and duration of exposure, looking for synergistic effects. Cell lines DU 145, PC 3, LnCaP, LL 86, MCF7wt, and MCF7/ADR (NCI/ADR-RES) were incubated with varying concentrations of paclitaxel or vinorelbine. Cytotoxicity was evaluated by a semiautomated MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) method. Synergism or antagonism of these two agents either sequentially or in combination was determined by median effect analysis. Prolonged exposure of cells to either drug enhanced cytotoxic effect. Synergism or antagonism with vinorelbine and paclitaxel were both sequence dependent and cell line specific. In the case of MCF7wt, synergism was seen when a 48-hr exposure to vinorelbine preceded paclitaxel, whereas antagonism was noted when both agents were applied simultaneously or when the sequence was reversed. Concurrent vinorelbine and paclitaxel were synergistic in four of six cell lines when the exposure was extended to 96 hr but not for shorter durations of exposure. Sequential exposure of vinorelbine preceding paclitaxel or prolonged exposure to both agents concurrently needs to be tested clinically to determine whether the antitumor activity of this combination can be enhanced. In addition, these studies suggest concurrent administration of these two agents may lead to a less than optimal cytotoxic result.
Fédération internationale des sociétés et instituts de la Renaissance. Colloque de San Francisco - 26 et 27 août 1975 (1975)

Béné Charles. Fédération internationale des sociétés et instituts de la Renaissance. Colloque de San Francisco - 26 et 27 août 1975. In: Bulletin de l'Association d'étude sur l'humanisme, la réforme et la renaissance, n°2, 1975. pp. 46-48.
Differences defined by bone marrow transplantation suggest that lpr and gld are mutations of genes encoding an interacting pair of molecules. (1990)

Homozygosity for either of the lymphoproliferation (lpr) or generalized lymphoproliferative disease (gld) mutations of mice causes the development of systemic lupus erythematosus-like autoimmune syndromes that are characterized by severe lymphadenopathy and highly elevated serum immunoglobulin levels. Although the mutations are nonallelic, analysis of homozygous lpr/lpr and gld/gld mice on the same strain background has indicated that the pathology and severity of the autoimmune syndromes induced by these mutations are indistinguishable. To explain this, it has previously been suggested that lpr and gld may represent mutations in molecules involved in sequential steps of an intracellular metabolic pathway of T cells. We have now investigated the behavior of both lpr and gld in a variety of bone marrow chimeras and have found that functional differences between lpr and gld become apparent after bone marrow transfer. Transfer of lpr/lpr bone marrow to irradiated congenic +/+ recipients caused the development of a graft-vs.-host-like lymphoid wasting syndrome, whereas transfer of gld/gld bone marrow to +/+ recipients resulted in development of a gld-like autoimmune syndrome. Additionally, gld/gld hosts behaved like +/+ hosts irrespective of the genotype of the donor bone marrow, whereas lpr/lpr hosts behaved unlike +/+ hosts when reconstituted with either lpr/lpr, gld/gld, or +/+ bone marrow. These are the first clear differences between these two mutations yet described. Our studies indicate that the molecule altered by the gld mutation is expressed only by bone marrow-derived cells, whereas the molecule altered by the lpr mutation is expressed by both bone marrow-derived cells and by one or more peripheral radioresistant cell populations. To reconcile these differences with the fact that homozygous lpr/lpr and gld/gld mice are indistinguishable, we suggest an alternative model for the relationship between the lpr and gld mutations in which the two molecules affected represent an interacting ligand-receptor pair expressed by different cells.
Implantation of Low-Level Laser Irradiated Mesenchymal Stem Cells into the Infarcted Rat Heart Is Associated with Reduction In Infarct Size and Enhanced Angiogenesis (2009)

The findings of the present study provide the first evidence that LLLT can significantly increase survival and/or proliferation of MSCs post-implantation into the ischemic/infarcted heart, followed by a marked reduction of scarring and enhanced angiogenesis. The mechanisms associated with this phenomenon remain to be elucidated in further studies.
IPP@HDL: Efficient Intellectual Property Protection Scheme for IP Cores (2007)

In this paper, a procedure for intellectual property protection (IPP) of digital circuits called IPP@HDL is presented. Its aim is to protect the author rights in the development and distribution of reusable modules by means of an electronic signature. The technique relies on hosting the bits of the digital signature within memory structures or combinational logic that are part of the system, at the high level description of the design. Thus, the area of the system is not increased and the signature is difficult to change or to remove without damaging the design. The technique also includes a procedure for secure signature extraction requiring minimal modifications to the system and without interfering its normal operation. The benefits of the presented procedure are illustrated with programmable logic and cell-based application-specific integrated circuit examples with several signature lengths. These design examples show no performance degradation and a negligible area increase, while probabilistic analyses show that the proposed IPP scheme offers high resistance against attacks.
Nanoparticle Plasmonics for 2D-Photovoltaics: Mechanisms, Optimization, and Limits (2009)

Plasmonic nanostructures placed within or near photovoltaic (PV) layers are of high current interest for improving thin film solar cells. We demonstrate, by electrodynamics calculations, the feasibility of a new class of essentially two dimensional (2D) solar cells based on the very large optical cross sections of plasmonic nanoparticles. Conditions for inducing absorption in extremely thin PV layers via plasmon near-fields, are optimized in 2D-arrays of (i) core-shell particles, and (ii) plasmonic particles on planar layers. At the plasmon resonance, a pronounced optimum is found for the extinction coefficient of the PV material. We also characterize the influence of the dielectric environment, PV layer thickness and nanoparticle shape, size and spatial distribution. The response of the system is close to that of a 2D effective medium layer, and subject to a 50% absorption limit when the dielectric environment around the 2D layer is symmetric. In this case, a plasmon induced absorption of about 40% is demonstrated in PV layers as thin as 10 nm, using silver nanoparticle arrays of only 1 nm effective thickness. In an asymmetric environment, the useful absorption may be increased significantly for the same layer thicknesses. These new types of essentially 2D solar cells are concluded to have a large potential for reducing solar electricity costs.
Advances in treatment of chronic myelogenous leukemia – new treatment options with tyrosine kinase inhibitors (2009)

Imatinib is considered standard therapy for patients with chronic myelogenous leukemia (CML), inducing a high rate of hematologic and cytogenetic responses. Despite these excellent results, several patients develop resistance to imatinib. Mechanisms of resistance are varied and include BCR-ABL1 kinase domain mutations, decreased entry of imatinib into cells, acquisition of secondary genetic changes and activation of alternate signaling pathways. Second-generation tyrosine kinase inhibitors (TKI) (dasatinib, nilotinib) were developed as an alternative for patients that develop resistance or are intolerant to imatinib. Dasatinib is a dual Abl/Src kinase TKI that is structurally unrelated to imatinib and is approved for therapy of all phases of CML in patients who are resistant or intolerant to imatinib. Nilotinib is a compound related to imatinib that has greater specificity and improved binding characteristics, and has clinical activity in the setting of imatinib failure. Resistance to multiple TKIs does occur, particularly in patients with the T315I mutation. Several new agents are in development including new TKIs, aurora kinase inhibitors and homoharringtonine.
Experimental inoculation of porcine circovirusestype 1 (PCV1) and type 2 (PCV2) in rabbits and mice (2002)

The objective of this work was to investigate the susceptibility of rabbits and mice experimentally inoculated with porcine circoviruses type 1 (PCV1) and type 2 (PCV2) to infection and development of disease and/or lesions. Forty six New Zealand rabbits and 50 ICR-CDI mice were both divided into two groups comprising PCVI and PCV2 inoculated animals, and a third group inoculated with non-infected cell culture medium. Rabbits were inoculated intranasally while mice were inoculated intraperitoneally. Clinical signs and body weights were recorded at the start of the experiment and at necropsy. Animals were bled, euthanised and necropsied at days 0, 3, 7, 10, 14 and 20 post-inoculation and samples were collected for histopathological, serological, in situ hybridisation and PCR analysis. No clinical signs or gross and microscopic lesions compatible with PCV2 infections such as those seen in pigs were observed. No presence of PCV2 nucleic acid was detected in rabbits and mice by in situ hybridisation. Only one mouse inoculated with PCV1 seroconverted on day 20 P1. PCV1 and PCV2 genome was detected in serum by PCR in mice inoculated with each porcine circovirus, while rabbits were negative for both viral types. These studies indicated that porcine circoviruses did not cause any disease or microscopic lesions in inoculated rabbits and mice during the experimental period. However, intraperitoneally inoculated mice might have harboured PCV2 in circulation without evidence of viral replication.
Predicting childhood obesity using electronic health records and publicly available data (2019)

We were able to predict obesity at age five using EHR data with an AUC comparable to cohort-based studies, reducing the need for investment in additional data collection. Our results suggest that machine learning approaches for predicting future childhood obesity using EHR data could improve the ability of clinicians and researchers to drive future policy, intervention design, and the decision-making process in a clinical setting.
A fully-integrated highly linear zero-IF CMOS cellular CDMA receiver (2005)

A 0.25 /spl mu/m highly linear zero-IF CMOS receiver with integrated LNA, downconverter, baseband filter, and VCO supports USA and Japanese CDMA applications. The receiver IC occupies 5.8mm/sup 2/ and draws 56mA at 2.5V.
Handbook on Radiation Probing, Gauging, Imaging and Analysis (2004)

The need for this book arose from my teaching, engineering, and - search experience in the non-power aspects of nuclear technology. The lack of a comprehensive textbook in industrial applications of radiation frustrated my students, who had to resort to a multitude of textbooks and research publications to familiarize themselves with the fundam- tal and practical aspects of radiation technology. As an engineer, I had to acquire the design aspects of radiation devices by trial-and-error, and often by accidental reading of a precious publication. As a researcher and a supervisor of graduate students, I found that the needed literature was either hard to find, or too scattered and diverse. More than once, I discovered that what appeared to be an exciting new idea was an old concept that was tried a few decades earlier during the golden era of "Atom for Peace". I am hoping, therefore, that this book will serve as a single comprehensive reference source in a growing field that I expect will continue to expand. This book is directed to both neophytes and experts, and is written to combine the old and the new, the basic and the advanced, the simple and the complex. It is anticipated that this book will be of help in - viving older concepts, improving and expanding existing techniques and promoting the development of new ones
Supporting highly speculative execution via adaptive branch trees (2002)

Most of the prediction mechanisms predict a single path to continue the execution on a branch. Alternatively, we may exploit parallelism from either possible paths of a branch, discarding wrong paths once the branch is resolved. This paper proposes a concept of adaptive branch trees (ABT) to support highly speculative execution for processors with deeper pipelines and wide issue widths. The basic idea of the adaptive branch tree is to dynamically keep track of alternative branch paths and to speculatively execute the code on the most likely path. Hence, unlike branch prediction mechanisms, the ABT scheme would not miss out misprediction paths since the scheme can eventually go back to other alternative paths when the machine has explored more pending branches. The branch tree is realized by an adaptive branch tree table. A token is associated with each basic block and operations in the entire basic blocks are tagged with the token. With a novel token assignment strategy, we can reconfigure the ABT by a shift operation once one branch is resolved. Our experiment results on the SPEC95 benchmarks show that the proposed approach can achieve significant branch penalty reduction for wide-issue processors.
Photochemical Reaction of Ubiquinone (35). VI. Coenzymatic Activity of Ubiquinone (35) and Related Compounds (1965)

1) Using the mitochondria treated with acetone, measurement was made about the activity-restoring effect of ubiquinone (35)(I) and related compounds on the succinate oxidase system, and as the partial structure required for the effect the following points were made clear : i) the quinone nucleus is necessary, ii) the methoxyl at position of 3 may be changed to a hydroxyl but if it is converted to acetoxyl the effect is lost, iii) the length of the isoprene side chain has no influence so long as the number of the isoprene is 7∼10, but when the double bonds of the side chain are saturated, the activity decreases to 1/2, and when one of the double bonds is conjugated with the quinone nucleus, the activity lowers to 1/3. 2) When added to beef-heart mitochondria, some substances in Table IV other than ubiquinone (35)(I) and ubichromenol (35) acetate (IX) show slight inhibition of the succinate oxidase system.
Primary sequence of the alpha-toxin gene from Staphylococcus aureus wood 46 (1984)

The complete DNA sequence of a cloned alpha-toxin gene from Staphylococcus aureus was determined. The amino acid sequence of the alpha-toxin protein, predicted from the DNA sequence, was described and compared with published data. The primary product of the cloned alpha-toxin gene contained a 26-amino-acid leader sequence which possessed characteristic features of a signal sequence involved in secretion. The mature alpha-toxin protein had a molecular size of 33,000 and contained only three short regions of high hydrophobicity in addition to a number of short, weakly hydrophobic regions.
Mixed-function oxidase involvement in the biochemistry of insecticide synergists (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMixed-function oxidase involvement in the biochemistry of insecticide synergistsJohn E. CasidaCite this: J. Agric. Food Chem. 1970, 18, 5, 753–772Publication Date (Print):May 1, 1970Publication History Published online1 May 2002Published inissue 1 May 1970https://pubs.acs.org/doi/10.1021/jf60171a013https://doi.org/10.1021/jf60171a013research-articleACS PublicationsRequest reuse permissionsArticle Views587Altmetric-Citations296LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Transfer of synaptic vesicle antigens to the presynaptic plasma membrane during exocytosis. (1981)

We have utilized immunofluorescence techniques to look for synaptic vesicle antigens on the plasma membrane of resting and active nerve terminals. Rabbit antiserum was raised against purified cholinergic synaptic vesicles from the electric organ of Narcine brasiliensis, a marine electric ray. Antibodies to synaptic vesicles were shown to bind selectively to nerve terminals in cryostat sections of frog nerve-muscle preparations. Binding was demonstrated indirectly by using fluorescein-labeled goat anti-rabbit antibodies. Structures in cross sections that bound antiserum were identified as nerve terminals because of their size, shape, and position and because they coincided with sites that bound rhodamine-conjugated alpha-bungarotoxin and had acetylcholine esterase activity. Presumably, sectioning gave antibodies access to binding sites within the nerve terminal. However, when antibodies to synaptic vesicles were added to the bathing medium of intact neuromuscular preparations prior to sectioning, antibody binding was marginal or undetectable, suggesting that few vesicle antigens were normally accessible on the outer surface of resting nerve terminals. When intact preparations were stimulated to release their vesicular acetylcholine by the addition of 1 mM LaCl3, antibody binding to the intact nerve terminals became striking. These findings suggest that the synaptic vesicle membrane and the synaptic terminal plasma membrane differ in composition. They also provide further support for the exocytotic hypothesis of neurotransmitter release, which predicts that vesicle markers should be exposed on the outside of nerve terminals when vesicles fuse with the plasma membrane during stimulation.
Photonic crystal fiber devices (2012)

The properties of photonic crystal fibers can be radically modified by deforming, enlarging and selective filling of the air-holes. Such post-processing would allow useful fiber devices such as polarizers, polarization controllers, in-fiber interferometers, and etc.
Dijkstra's Shortest Path Algorithm (2003)

Summary. The article formalizes Dijkstra’s shortest path algorithm [11]. A path from a source vertex v to a target vertex u is said to be the shortest path if its total cost is minimum among all v-to-u paths. Dijkstra’s algorithm is based on the following assumptions: • All edge costs are non-negative. • The number of vertices is finite. • The source is a single vertex, but the target may be all other vertices. The underlying principle of the algorithm may be described as follows: the algorithm starts with the source; it visits the vertices in order of increasing cost, and maintains a set V of visited vertices (denoted by UsedVx in the article) whose cost from the source has been computed, and a tentative cost D(u) to each unvisited vertex u. In the article, the set of all unvisited vertices is denoted by UnusedVx. D(u) is the cost of the shortest path from the source to u in the subgraph induced by V ∪ {u}. We denote the set of all unvisited vertices whose Dvalues are not infinite (i.e. in the subgraph each of which has a path from the source to itself) by OuterVx. Dijkstra’s algorithm repeatedly searches OuterVx for the vertex with minimum tentative cost (this procedure is called findmin in the article), adds it to the set V and modifies