gebhart/patrepeval huggingface dataset:
Trypsinization-dependent cell labeling with fluorescent nanoparticles (2014)

Trypsin is often used to detach adhered cell subculture from a substrate. However, the proteolytic activity of trypsin may harm cells by cleaving the cell membrane proteins. The present study shows that cellular uptake of fluorescent nanoparticles is remarkably increased within 24 h after trypsinization. These results highlight the trypsin-induced protein digestion, provoking leaky cell plasma membrane which leads to the strongly enhanced cellular uptake of the nanoparticles. To prevent this effect, one should expose cells to the nanoparticle (NP)-based fluorescent labels at least 48 h after trypsinization.
Crystal Structure of a Polyhistidine-Tagged Recombinant Catalytic Subunit of cAMP-Dependent Protein Kinase Complexed with the Peptide Inhibitor PKI(5−24) and Adenosine (1997)

The crystal structure of the hexahistidine-tagged mouse recombinant catalytic subunit (H6-rC) of cAMP-dependent protein kinase (cAPK), complexed with a 20-residue peptide inhibitor from the heat-stable protein kinase inhibitor PKI(5-24) and adenosine, was determined at 2.2 A resolution. Novel crystallization conditions were required to grow the ternary complex crystals. The structure was refined to a final crystallographic R-factor of 18.2% with good stereochemical parameters. The "active" enzyme adopts a "closed" conformation as found in rC:PKI(5-24) [Knighton et al. (1991a,b) Science 253, 407-414, 414-420] and packs in a similar manner with the peptide providing a major contact surface. This structure clearly defines the subsites of the unique nucleotide binding site found in the protein kinase family. The adenosine occupies a mostly hydrophobic pocket at the base of the cleft between the two lobes and is completely buried. The missing triphosphate moiety of ATP is filled with a water molecule (Wtr 415) which replaces the gamma-phosphate of ATP. The glycine-rich loop between beta1 and beta2 helps to anchor the phosphates while the ribose ring is buried beneath beta-strand 2. Another ordered water molecule (Wtr 375) is pentacoordinated with polar atoms from adenosine, Leu 49 in beta-strand 1, Glu 127 in the linker strand between the two lobes, Tyr 330, and a third water molecule, Wtr 359. The conserved nucleotide fold can be defined as a lid comprised of beta-strand 1, the glycine-rich loop, and beta-strand 2. The adenine ring is buried beneath beta-strand 1 and the linker strand (120-127) that joins the small and large lobes. The C-terminal tail containing Tyr 330, a segment that lies outside the conserved core, covers this fold and anchors it in a closed conformation. The main-chain atoms of the flexible glycine-rich loop (residues 50-55) in the ATP binding domain have a mean B-factor of 41.4 A2. This loop is quite mobile, in striking contrast to the other conserved loops that converge at the active site cleft. The catalytic loop (residues 166-171) and the Mg2+ positioning loop (residues 184-186) are a stable part of the large lobe and have low B-factors in all structures solved to date. The stability of the glycine-rich loop is highly dependent on the ligands that occupy the active site cleft with maximum stability achieved in the ternary complex containing Mg x ATP and the peptide inhibitor. In this ternary complex the gamma-phosphate is secured between both lobes by hydrogen bonds to the backbone amide of Ser 53 in the glycine-rich loop and the amino group of Lys 168 in the catalytic loop. In the adenosine ternary complex the water molecule replacing the gamma-phosphate hydrogen bonds between Lys 168 and Asp 166 and makes no contact with the small lobe. This glycine-rich loop is thus the most mobile component of the active site cleft, with the tip of the loop being highly sensitive to what occupies the gamma-subsite.
Specific Detection of <i>Aspergillus</i> Species in Blood and Bronchoalveolar Lavage Samples of Immunocompromised Patients by Two-Step PCR (1999)

The increasing incidence of aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the currently limited diagnostic tools. We developed a two-step PCR assay that specifically amplifies a region of the 18S rRNA gene that is highly conserved in Aspergillus species. A number of primers with the least homology to equivalent human or Candida gene sequences were screened for the pairs that gave the highest sensitivity and specificity. No cross-reaction with the wide range of fungal and bacterial pathogens so far tested was observed. This assay allows direct and rapid detection of down to 10 fg of Aspergillus DNA corresponding to 1 to 5 CFU per ml of blood. A total of 315 blood and bronchoalveolar lavage samples from 140 subjects, including 93 patients at risk for invasive fungal disease, were screened. The result was a 100% correlation between positive histology, culture, or high-resolution computed tomography findings and PCR results. The test specificity was 89%. Our data point to the considerable potential clinical value of this simple, specific, rapid, and inexpensive PCR assay for improving the means of early diagnosis of systemic aspergillosis in high-risk patients.
Bilayer Membranes of Four-Chained Ammonium Amphiphiles (1990)

Abstract Novel amphiphiles in which four long alkyl chains are connected to the single trimethylammonium head group via amino acid residues gave clear dispersions upon sonication. Formation of bilayer membranes was confirmed by electron microscopy, differential scanning calorimetry, and fluorescence depolarization.
Involvement of Tissue Factor Pathway Inhibitor in the Coronary Circulation of Patients With Acute Coronary Syndromes (2003)

TFPI is involved in the process of thrombus formation in vivo in patients with ACS, which suggests a potential role for TFPI in modulating coronary thrombosis.
A Kernel Classification Framework for Metric Learning (2013)

Learning a distance metric from the given training samples plays a crucial role in many machine learning tasks, and various models and optimization algorithms have been proposed in the past decade. In this paper, we generalize several state-of-the-art metric learning methods, such as large margin nearest neighbor (LMNN) and information theoretic metric learning (ITML), into a kernel classification framework. First, doublets and triplets are constructed from the training samples, and a family of degree-2 polynomial kernel functions are proposed for pairs of doublets or triplets. Then, a kernel classification framework is established, which can not only generalize many popular metric learning methods such as LMNN and ITML, but also suggest new metric learning methods, which can be efficiently implemented, interestingly, by using the standard support vector machine (SVM) solvers. Two novel metric learning methods, namely doublet-SVM and triplet-SVM, are then developed under the proposed framework. Experimental results show that doublet-SVM and triplet-SVM achieve competitive classification accuracies with state-of-the-art metric learning methods such as ITML and LMNN but with significantly less training time.
L'extrait sec et le rapport alcool/extrait des vins suisses (1973)

Les auteurs ont analysé 424 vins blancs rouges et rosés tous originaires de Suisse et ils ont déterminé la répartition statistique du rapport alcool/extrait sec réduit. Ils ont montré que la valeur limite de ce rapport, fixée à 4 pour les vins rouges, permettait de mettre en évidence un enrichissement du vin rouge par sucrage du moût. La valeur de 5,6, admise en France pour les vins blancs secs, par contre, ne permet pas de détecter le sucrage des blancs suisses. Les auteurs ont en outre pu montrer la précision, l'exactitude et la reproductibilité des méthodes d'analyse densimétrique de l'extrait total par pycnométrie (Méthode directe et méthode de Tabarié).
Double-blind Parallel Design Pilot Study of Acetyl Levocarnitine in Patients With Alzheimer's Disease (1992)

Acetyl levocarnitine hydrochloride has been reported to retard dementia in patients with Alzheimer's disease. In a double-blind, parallel design, placebo-controlled pilot study of 30 mild to moderately demented patients with probable Alzheimer's disease, tests of memory, attention, language, visuospatial, and constructional abilities were administered, and the level of acetyl levocarnitine was measured in the cerebrospinal fluid. Patients were then randomly assigned to receive acetyl levocarnitine hydrochloride (2.5 g/d for 3 months followed by 3 g/d for 3 months) or placebo. After 6 months, the acetyl levocarnitine group demonstrated significantly less deterioration in timed cancellation tasks and Digit Span (forward) and a trend toward less deterioration in a timed verbal fluency task. No differences were found in any other neuropsychological test results. A subgroup with the lowest baseline scores, receiving acetyl levocarnitine, had significantly less deterioration on the verbal memory test and a significant increase in cerebrospinal fluid acetyl levocarnitine levels compared with those receiving placebo. These results suggest that acetyl levocarnitine may retard the deterioration in some cognitive areas in patients with Alzheimer's disease and stress the need for a larger study of this drug.
Purification of Antibodies by Chromatographic Methods (2004)

Antibodies belong to the immunoglobulin family, i.e. a class of proteins that constitute the so-called humoral branch of the immune system. They represent approximately 20 % of the proteins in human blood plasma. Besides their physiological function, antibodies are also important tools for general research as well as for the diagnosis and treatment of a vast array of diseases. This is largely due to the fact that antibodies can be purposedly raised against many natural and synthetic molecules, which they later recognize in a highly specific way, even in very complex matrices.
Research on casing wear monitoring technique based on detection of tool joints and its field test (2011)

Casing wear monitoring is basis of scientific casing wear prevention work, and now there is no scientific and convenient casing wear monitoring techniques in current drilling engineering. Wear characteristics of the casing — drill pipe system is further analyzed, basic theory and analysis methods of casing wear monitoring based on detection of tool joints are established through reasonable assumptions, and corresponding computer software is developed to form a complete set of casing wear monitoring technique which achieves quantitative calculation of casing wear at any depth of the well. Field tests indicate that this casing wear monitoring technique based on detection of tool joints can calculate the well casing wear volume (or weight) at any depth easily, its monitoring and computation are simple and the process has no impact on drilling, so it is suitable method for field operations.
Self sustaining bicycle robot with steering controller (2004)

Bicycle is a transportation device without any environmental burden. However, bicycle is unstable in itself and it is fall down without human assistance like steering handle or moving upper body. In these days, electric power assistance bicycles are used practically, but all of those bicycles merely assist human with pedal driving and there are no bicycles that help to stabilize its position. Hence, stabilizing the posture and realizing stable driving of a bicycle have been researched. Dynamic model of running bicycle is complicated and it's hard to recognize completely. However, assuming that the rider doesn't move upper body, dynamics of bicycle is represented in equilibrium of gravity and centrifugal force. Centrifugal force is risen out from the running velocity and turning radius determined by steering angle. Under these conditions, it is possible to stabilize bicycle posture by controlling its steering. In this paper, the dynamic model derived from equilibrium of gravity and centrifugal force is proposed. Then the control method for bicycle steering based on acceleration control is proposed. Finally, the validity of this method is proved by the simulations and experimental results.
Rational Synthesis and Structural Characterizations of Complex TiSi<sub>2</sub> Nanostructures (2009)

We present in this article our successes in synthesizing TiSi2 nanostructures with various complexities using a chemical vapor deposition (CVD) method. Attention has been paid to understanding the growth mechanism. The governing factor was found to be the surface energy differences between various crystal planes of orthorhombic TiSi2 (C54 and C49), because of their specific atomic arrangements of Si and Ti on the surfaces. This understanding has allowed us to control the growth morphologies and obtained one-dimensional (1D) nanowires, two-dimensional (2D) nanonets and three-dimensional (3D) complexes with rational designs by tuning the precursor chemical reactions. Careful studies of the atomically-resolved microstructures revealed the existence of distorted C54 phases in 3D complexes, which was attributed to playing the key role in the unique structure formation. These results are expected to shed light on metal silicide nanostructure growths broadly and to present opportunities for novel nanostructure syntheses.
Embolization of cerebral arteriovenous malformations with n-butyl-2-cyanoacrylate. (2000)

Endovascular treatment of brain AVMs with an NBCA mixture has a lower complication rate than shown in previous studies using other materials. The percentage of obliteration of the nidus is increased because NBCA penetrates AVMs better than polyvinyl alcohol particles. NBCA can be used at a lower concentration than other acrylates, and therefore causes almost no catheter gluing. The embolic mass formed by NBCA is more biocompatable than that formed by other acrylates.
Nicotine pharmacokinetics and its application to intake from smoking. (1985)

Five subjects were given 25 micrograms/kg nicotine intravenously over 1 min, before and after a loading period involving the smoking of six cigarettes. Plasma nicotine concentrations declined in a biphasic manner, the half-lives of the initial and terminal phases averaging 9 min and 133 min respectively. Terminal half-lives before and after the loading period were essentially the same suggesting the absence of saturation kinetics at nicotine concentrations that build up during smoking. The plasma clearance of nicotine and the volume of distribution were very high averaging 915 ml/min and 1731, respectively. Two approaches were used to calculate the nicotine intake from smoking. The average dose of nicotine absorbed from one cigarette was 1.06 mg which was 82% of the standard machine-smoked yield of 1.3 mg. To illustrate their potential use in 'nicotine titration' studies, these approaches were used to compare nicotine intake from smoking a high (2.4 mg) and low (0.6 mg) nicotine cigarette. The dose of nicotine absorbed averaged 1.14 mg and 0.86 mg per cigarette respectively, being 48% and 143% of the machine-smoked yields.
Modified phenol chemical face peels: recognizing the role of application technique. (2001)

The use of phenol to achieve chemexfoliation of facial tissue, following the standard Baker-Gordon formula or modifications of that formula, can produce a range of results depending on a variety of factors. This article-based on 16 new cases of modified phenol peels, three human biopsy studies, and one triple-blind study-examines the relative roles of phenol concentration, croton oil concentration, and application technique in the production of the chemical peel result. Results of this data suggest that the application technique is more important than the concentration of phenol or croton oil and underscore the importance of standardizing the application process.
The Shannon channel capacity of dispersion-free nonlinear optical fiber transmission (2001)

We extend the Shannon information theory to a nonlinear system and present a model for calculating the channel capacity of an optical-fiber transmission system using dispersion-free fiber. For this particular fiber, a closed-form solution for the nonlinear Schroedinger equation exists. This allows us to derive an analytical result for the channel capacity that is exact and valid for arbitrary input power. We will study the single-span case and examine the dependence of the capacity on operating input power, the number of channels (N/sub c/), the noise power (P/sub W/), etc. The maximum capacity is shown to follow a simple scaling law with log/sub 2/(1+CN/sub c//sup -2/3/P/sub W//sup -2/3/) dependence, multiplexing (WDM) systems.
Durkheim and Postmodern Culture. By Stjepan G. Mestrovic. Aldine de Gruyter, 1992. 187 pp. Cloth, $38.95; paper, $20.95 (1993)

Durkheim and Postmodern Culture.By Stjepan G. Meštrović. Aldine de Gruyter, 1992. 187 pp. Cloth, $38.95; paper, $20.95 Get access Ira J. Cohen Ira J. Cohen Rutgers University Search for other works by this author on: Oxford Academic Google Scholar Social Forces, Volume 72, Issue 1, September 1993, Pages 265–266, https://doi.org/10.1093/sf/72.1.265 Published: 01 September 1993
Crossword Compilation Using Integer Programming (1989)

Attention has been given over the last few years to the problem of efficiently compiling a crossword puzzle using a computerised algorithm. This paper considers the problem and formulates it as an integer program. The process of solving the integer program using standard commercial software provides some insight but other simpler approaches to crossword compilation appear to be preferable.
Good Word Attacks on Statistical Spam Filters. (2005)

This paper is concerned with the properties of a stochastic integral which arises in the study of a modified Markov branching process. Explicit expressions are found for the mean and the limit distribution of the integral.
Long-term prognosis of symptomatic lacunar infarcts. A hospital-based study. (1994)

Our study suggests that with a high survival rate, a low recurrence rate, and a relatively good functional recovery, lacunar infarcts have a relatively favorable prognosis.
Management of the patient with acute myocardial infarction (1989)

David Thompson looks at the goals designed to help patients return to normal life after acute myocardial infarction
Detection of number of sources via exploitation of centro-symmetry property (1994)

Forward/backward (FB) averaging is a technique which can be employed in sensor array signal processing for exploiting a so-called centro-symmetry property of the signal subspace to improve parameter estimation accuracy. Although FB averaging effectively doubles the number of data samples, it also introduces correlation among these data vectors. This correlation complicates not only the performance analysis, but also the detection of the number of sources. When FB averaging is employed, existing detection procedures such as sequential hypothesis (SH) likelihood ratio tests, minimum description length (MDL), and an information criterion (AIC) must be modified to correctly account for this preprocessing of the data. Herein, proper exploitation of the centro-symmetry property of the FB averaged covariance is described and a rigorous analysis is conducted. It is shown that the general scheme of existing methods is still applicable to the FB averaging case, but that the parameters (e.g., degrees of freedom) need to be adjusted.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Microgel‐Stabilized Metal Nanoclusters: Size Control by Microgel Nanomorphology (2003)

Soluble crosslinked macromolecules (microgels) used as stabilizers of catalytically active metal nanoclusters allow control of the nanocluster size by simply changing the crosslinking degree of the microgel. Microgel‐stabilized metal nanoclusters are easily prepared and stored in the solid state, and those of Pd (see Figure) exhibit high catalytic activity in the Heck reaction of activated aryl bromides.
A brief history of the Wason selection task (2016)

My talk at Ken Manktelow’s Festschrift conference was titled ‘Whatever happened 
to the selection task?’. However, some recent study of Web of Science has convinced me that my reports of its demise were somewhat exaggerated. While certainly past its prime, research on the task continues to feature in a number of recent 
publications, as we shall see. What the task has contributed overall to the psychology 
of reasoning in its 50 odd years of life is, however, quite remarkable. I offer here a 
brief, selective, affectionate and doubtless biased account of its history.
Photo Essay: The Rio Grande Borderland (1990)

The Rio Grande border of the United States and Mexico is over 1,000 miles long. This presentation of that border is by means of a photo essay interpreting the physical, economic, political, and cultural complex that has evolved. These photos were all taken in 1990 on a reconnaissance that followed both sides of the border for the entire distance from Ciudad Juarez and El Paso on the west to the Gulf of Mexico on the east. Numerous crossings of the border were made at official and non-official points. Informal field interviews were conducted on both sides of the border with government officials, agricultural workers, tourists, farmers, industrial managers, factory laborers, retirees, undocumented migrants, beachcombers, shoe-shiners in the plaza, sportsmen, clergymen, children, and loafers. The photos with an accompanying text are presented in an order that is conducive to a comprehension of the salient aspects of the physical and human geography and the complex social issues found in the borderland. The final photos and text are on the maquiladoras and the international interplay of history, markets, labor, and technology resulting in striking structural change.
Prediction Accuracy Measures for a Nonlinear Model and for Right-Censored Time-to-Event Data (2016)

This paper studies prediction summary measures for a prediction function under a general setting in which the model is allowed to be misspecified and the prediction function is not required to be the conditional mean response. We show that the R2 measure based on a variance decomposition is insufficient to summarize the predictive power of a nonlinear prediction function. By deriving a prediction error decompo- sition, we introduce an additional measure, L2, to augment the R2 measure. When used together, the two measures provide a complete summary of the predictive power of a prediction function. Furthermore, we extend these measures to right-censored time-to-event data by establishing right-censored data analogs of the variance and prediction error decompositions. We illustrate the usefulness of the proposed mea- sures with simulations and real data examples. Supplementary materials for this article are available online.
Vorinostat and Bortezomib Synergistically Cause Ubiquitinated Protein Accumulation in Prostate Cancer Cells (2012)

Vorinostat and bortezomib inhibit the growth of prostate cancer cells synergistically by causing ubiquitinated proteins to accumulate in cells. The current study provides a framework for testing the combination in patients with advanced prostate cancer.
NOTES ON THE POSITIVE HYDROTROPISM OF GERRIS MARGINATUS SAY AND DINEUTES ASSIMILIS AUBE (1914)

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