gebhart/patrepeval huggingface dataset:
Effect of chelating agents and metal ions on the degradation of DNA by bleomycin (1978)

The degradation of DNA by bleomycin was studied in the absence and in the presence of added reducing agents, including 2-mercaptoethanol, dithiothreitol, reduced nicotinamide adenine dinucleotide phosphate, H2O2, and ascorbate, and in the presence of a superoxide anion generating system consisting of xanthine oxidase and hypoxanthine. In all cases, breakage of DNA was inhibited by low concentrations of chelators; where examined in detail, deferoxamine mesylate was considerably more potent than (ethylenedinitrilo)tetraacetic acid. Iron was found to be present in significant quantities in all reaction mixtures. Thus, the pattern of inhibition observed is attributed to the involvement of contaminating iron in the degradation of DNA by bleomycin. Cu(II), Zn(II), and Co(II) inhibit degradation of DNA by bleomycin and Fe(II) in the absence of added reducing agents. A model is proposed in which the degradation of DNA in these systems is dependent on the oxidation of an Fe(II)-bleomycin-DNA complex.
24-Hour ambulatory blood pressure monitoring in primary care. (2001)

This project shows that a 24-hour ABPM consultation service can provide useful information for determining which patients have isolated office hypertension and in guiding drug regimen modification for patients with diabetes, suspected resistant hypertension, or drug-induced alterations in blood pressure.
Genetic and biochemical analyses of yeast TATA-binding protein mutants. (1993)

We have taken a combined genetic and biochemical approach to study TATA-binding protein (TBP) structure-function relationships. Using site-directed mutagenesis coupled with a screen for conditional lethal growth, we have isolated a number of temperature-sensitive TBP alleles in the region of amino acid positions 188, 189, and 190. Conditional growth is not a result of increased TBP turnover as most of the mutant proteins are stable in vivo as evidenced by immunoblot detection of TBP steady-state levels. DNA binding assays reveal that mutations at position 188 do not affect DNA binding activity of these mutants, even at high temperatures. Utilizing whole cell extracts which contain mutant TBPs in in vitro transcription experiments, we confirm that TBP is required for transcription by all three nuclear polymerases. However, certain of our TBP mutants are only compromised for RNA polymerase II transcription.
Topography of obliquely evaporated silicon oxide films and its effect on liquid-crystal orientation (1977)

The topography of silicon oxide films deposited at an angle of incidence of either 60° or 83° has been investigated using transmission electron microscopy. Columnar-growth structure arrayed in rowlike order has been detected for films deposited at both angles of incidence. The columns lie in the plane of incidence in both cases. The column angle of inclination from the normal to the substrate is 35° for the 60° deposition and it is 50° for the 83° deposition. The rows are, on the average, perpendicular to the plane of incidence. The ratio of the column width to the column periodicity is 0.70 for the 60° evaporation and is about 0.20-0.25 for films deposited at an angle of incidence of 83°. It is asserted that the topography of films deposited at the two angles is the primary factor determining the directional orientation of liquid crystals on obliquely deposited silicon oxide.
Immune-response profiles induced by human immunodeficiency virus type 1 vaccine DNA, protein or mixed-modality immunization: increased protection from pathogenic simian–human immunodeficiency virus viraemia with protein/DNA combination (2008)

Current data suggest that prophylactic human immunodeficiency virus type 1 (HIV) vaccines will be most efficacious if they elicit a combination of adaptive humoral and T-cell responses. Here, we explored the use of different vaccine strategies in heterologous prime-boost regimes and evaluated the breadth and nature of immune responses in rhesus monkeys induced by epidermally delivered plasmid DNA or recombinant HIV proteins formulated in the AS02A adjuvant system. These immunogens were administered alone or as either prime or boost in mixed-modality regimes. DNA immunization alone induced cell-mediated immune (CMI) responses, with a strong bias towards Th1-type cytokines, and no detectable antibodies to the vaccine antigens. Whenever adjuvanted protein was used as a vaccine, either alone or in a regime combined with DNA, high-titre antibody responses to all vaccine antigens were detected in addition to strong Th1- and Th2-type CMI responses. As the vaccine antigens included HIV-1 Env, Nef and Tat, as well as simian immunodeficiency virus (SIV)mac239 Nef, the animals were subsequently exposed to a heterologous, pathogenic simian-human immunodeficiency virus (SHIV)89.6p challenge. Protection against sustained high virus load was observed to some degree in all vaccinated groups. Suppression of virus replication to levels below detection was observed most frequently in the group immunized with protein followed by DNA immunization, and similarly in the group immunized with DNA alone. Interestingly, control of virus replication was associated with increased SIV Nef- and Gag-specific gamma interferon responses observed immediately following challenge.
The Good Earth (1996)

In january, 1931, Lossing and Pearl spent several weeks sailing south along the Chinese coast toward Hong Kong and Canton (Guangzhou). Lossing was trying to make arrangements for an expanded survey of agriculture, while Pearl was exploring parts of China she had not seen before. Several times, the Bucks nearly stepped into the fighting that swept back and forth across the countryside, devastating thousands of farms and keeping the peasants in a constant state of terror. Armed struggle had spread everywhere. Bandits were fighting Chiang Kaishek's Kuomintang troops and each other, the KMT was battling Communists as well as local warlords, Communists were harassing KMT soldiers at every opportunity and also attacking bandits and landlords. On more than one occasion, the armed men who fought and killed each other literally did not know who their enemies had been. The slide into luan, chaos, which the Chinese feared above all else, seemed to have become irreversible.
Hypercalcitoninemia, Hypocalcemia, and Toxic Shock Syndrome (1990)

Toxic shock syndrome is a multisystem illness frequently complicated by hypocalcemia. The etiology of the hypocalcemia, which may be severe, is not well understood. We report two cases of fatal toxic shock syndrome accompanied by severe hypocalcemia; each patient also had an inappropriately elevated serum calcitonin level, which in one case was as high as 179,000 pg/mL. Hypercalcitoninemia may be a cause of the low serum calcium levels as well as of certain clinical manifestations of toxic shock syndrome.
Messianic Motifs of Qumran and the New Testament (1956)

The relationship between the New Testament and the Qumran Literature is much more basic and fundamental than occasional words and phrases reminiscent of one another; for the messianic motifs of the Qumran Community are combined, modified, and adapted in the New Testament portrayal of Jesus the Christ. As we here use the term Messianic, we intend to include all that relates to the great eschatological figures of the sect, the Teacher of Righteousness, the coming prophet like unto Moses, and the two Messiahs of Aaron and Israel.
Frequent Detection of Escape from Cytotoxic T-Lymphocyte Recognition in Perinatal Human Immunodeficiency Virus (HIV) Type 1 Transmission: the Ariel Project for the Prevention of Transmission of HIV from Mother to Infant (1999)

Host immunologic factors, including human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL), are thought to contribute to the control of HIV type 1 (HIV-1) replication and thus delay disease progression in infected individuals. Host immunologic factors are also likely to influence perinatal transmission of HIV-1 from infected mother to infant. In this study, the potential role of CTL in modulating HIV-1 transmission from mother to infant was examined in 11 HIV-1-infected mothers, 3 of whom transmitted virus to their offspring. Frequencies of HIV-1-specific human leukocyte antigen class I-restricted CTL responses and viral epitope amino acid sequence variation were determined in the mothers and their infected infants. Maternal HIV-1-specific CTL clones were derived from each of the HIV-1-infected pregnant women. Amino acid substitutions within the targeted CTL epitopes were more frequently identified in transmitting mothers than in nontransmitting mothers, and immune escape from CTL recognition was detected in all three transmitting mothers but in only one of eight nontransmitting mothers. The majority of viral sequences obtained from the HIV-1-infected infant blood samples were susceptible to maternal CTL. These findings demonstrate that epitope amino acid sequence variation and escape from CTL recognition occur more frequently in mothers that transmit HIV-1 to their infants than in those who do not. However, the transmitted virus can be a CTL susceptible form, suggesting inadequate in vivo immune control.
Efficient joint 2D and 3D palmprint matching with alignment refinement (2010)

Palmprint verification is a relatively new but promising personal authentication technique for its high accuracy and fast matching speed. Two dimensional (2D) palmprint recognition has been well studied in the past decade, and recently three dimensional (3D) palmprint recognition techniques were also proposed. The 2D and 3D palmprint data can be captured simultaneously and they provide different and complementary information. 3D palmprint contains the depth information of the palm surface, while 2D palmprint contains plenty of textures. How to efficiently extract and fuse the 2D and 3D palmprint features to improve the recognition performance is a critical issue for practical palmprint systems. In this paper, an efficient joint 2D and 3D palmprint matching scheme is proposed. The principal line features and palm shape features are extracted and used to accurately align the palmprint, and a couple of matching rules are defined to efficiently use the 2D and 3D features for recognition. The experiments on a 2D+3D palmprint database which contains 8000 samples show that the proposed scheme can greatly improve the performance of palmprint verification.
Exploring information visualization (2010)

Interactive Information Visualization methods engage users in exploratory behavior. Detailed information about such processes can help developers to improve the design of such methods. The following study which is based on software logging describes patterns of such behavior in more detail. Subjects in our study engaged in some activities (e.g. adding data, changing form of visualization) significantly more than in others. They adapted their activity patterns to different tasks, but not fundamentally so. In addition, subjects adopted very systematic sequences of actions. These sequences were quite similar across the whole sample, thus indicating that such sequences might reflect specific problem solving behavior. Davidson's [7] framework of problem solving behavior is used to interpret the results. More research is necessary to show whether similar interaction patterns can be found for the usage of other InfoVis methodologies as well.
Cloning and over‐expression in <i>Escherichia coli</i> of the gene encoding NADPH group III alcohol dehydrogenase from <i>Thermococcus hydrothermalis</i> (1999)

A NADP-dependent group III alcohol dehydrogenase (ADH) was purified from the hyperthermophilic strictly anaerobic archaeon Thermococcus hydrothermalis, which grows at an optimum temperature of 85 degrees C and an optimum pH of 6. The gene encoding this enzyme was cloned, sequenced, and over-expressed in Escherichia coli. The recombinant enzyme was purified, characterized and compared with the native form of the enzyme. The enzyme structure is pH-dependent, being a 197-kDa tetramer (subunit of 45 kDa) at pH 10.5, the pH optimum for alcohol oxidation, and a 80.5-kDa dimer at pH 7.5, the pH optimum for aldehyde reduction. The kinetic parameters of the enzyme show that the affinity of the enzyme is greater for the aldehyde substrate and NADPH cofactor, suggesting that the dimeric form of the enzyme is probably the active form in vivo. The ADH of T. hydrothermalis oxidizes a series of primary aliphatic and aromatic alcohols preferentially from C2 to C8 but is also active towards methanol and glycerol and stereospecific for monoterpenes. T. hydrothermalis ADH is the first Thermococcale ADH to be cloned and overproduced in a mesophilic heterologous expression system, and the recombinant and the native forms have identical main characteristics.
Access to Enantiopure α-Hydrazino Acids for <i>N</i>-Amino Peptide Synthesis (2017)

Backbone N-methylation of α-peptides has been widely employed to enhance the bioavailability and bioactivity of parent sequences. Heteroatomic peptide amide substituents have received less attention due, in part, to the lack of practical synthetic strategies. Here, we report the synthesis of α-hydrazino acids derived from 19 out of the 20 canonical proteinogenic amino acids and demonstrate their use in the solid-phase synthesis of N-amino peptide derivatives.
A dominant-negative pleiotrophin mutant introduced by homologous recombination leads to germ-cell apoptosis in male mice (1999)

Pleiotrophin (PTN) is an 18-kDa heparin-binding secretory growth/differentiation factor for different cell types. Its gene is differentially expressed in both mesenchyme and central nervous system during development and highly expressed in a number of different human tumors. Recently, a PTN mutant was found to act as a dominant-negative effector of PTN signaling. We have now used homologous recombination to introduce the dominant-negative PTN mutant into embryonic stem cells to generate chimeric mice. All highly chimeric male mice with germinal epithelium exclusively derived from embryonic stem cells with the heterologous PTN mutation were sterile. Their testes were uniformly atrophic, and the spermatocytes were strikingly apoptotic at all stages of development. The results support a central role of PTN signaling in normal spermatogenesis and suggest that interruption of PTN signaling may lead to sterility in males.
Fault Detection and Classification in Transmission Lines Based on Wavelet Transform and ANN (2006)

This paper proposes a novel method for transmission-line fault detection and classification using oscillographic data. The fault detection and its clearing time are determined based on a set of rules obtained from the current waveform analysis in time and wavelet domains. The method is able to single out faults from other power-quality disturbances, such as voltage sags and oscillatory transients, which are common in power systems operation. An artificial neural network classifies the fault from the voltage and current waveforms pattern recognition in the time domain. The method has been used for fault detection and classification from real oscillographic data of a Brazilian utility company with excellent results
A Comparison Study of Multiple Measures of Adherence to HIV Protease Inhibitors (2001)

Different measures applied to the same patient suggest different levels of adherence. Adherence may be underestimated by MEMS and overestimated by pill count and interview. A summary measure combining several measures is more strongly related to a clinical response, but more practical measurement methods are needed for clinical use.
Space-Time Joint Multi-layer Segmentation and Depth Estimation (2012)

Video-based segmentation and reconstruction techniques are predominantly extensions of techniques developed for the image domain treating each frame independently. These approaches ignore the temporal information contained in input videos which can lead to incoherent results. We propose a framework for joint segmentation and reconstruction which explicitly enforces temporal consistency by formulating the problem as an energy minimisation generalised to groups of frames. The main idea is to use optical flow in combination with a confidence measure to impose robust temporal smoothness constraints. Optimisation is performed using recent advances in the field of graph-cuts combined with practical considerations to reduce run-time and memory consumption. Experimental results with real sequences containing rapid motion demonstrate that the method is able to improve spatio-temporal coherence both in terms of segmentation and reconstruction without introducing any degradation in regions where optical flow fails due to fast motion.
Modeling complex astrophysics data (2002)

Scientific data are often characterized by large volumes of data, complex data relationships, and complex algorithms that operate on those data. Databases at the Space Telescope Science Institute are used to illustrate some of the differences between traditional data management schemes and the requirements of scientific data. Data from radio astronomy are modeled using conceptual enhancements to flexible image transport system (FITS) binary tables, which are compared to relational database tables. These enhancements are used to capture features of a more general notion of an observation. Finally, it is argued that accessing and manipulating each data, in order to support the entire process of data analysis, requires the construction of an abstract data model that intuitively models how a domain specialist views the data.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Interaction of an apolipoprotein (apoLP-alanine) with phosphatidylcholine (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteraction of an apolipoprotein (apoLP-alanine) with phosphatidylcholineJoel D. Morrisett, Joshua S. K. David, Henry J. Pownall, and Antonio M. Gotto, Jr.Cite this: Biochemistry 1973, 12, 7, 1290–1299Publication Date (Print):March 27, 1973Publication History Published online1 May 2002Published inissue 27 March 1973https://pubs.acs.org/doi/10.1021/bi00731a008https://doi.org/10.1021/bi00731a008research-articleACS PublicationsRequest reuse permissionsArticle Views743Altmetric-Citations261LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
ROBOTICS FOR ASSEMBLY, INSPECTION, AND MAINTENANCE OF SPACE MACROFACILITIES (2000)

complex, sometimes fragile and often less accessible than the satellites and space stations of today. The need is for AIM robots to assemble, inspect, and maintain such facilities, but significant technical challenges exist. This paper profiles early results towards extensive, constrained, and humanoid approaches to AIM robots. Like the visionary facilities these robots will enable, visionary AIM robots call for their own significant development campaign.
DOES HYPERTRIGLYCERIDEMIA INCREASE RISK FOR CAD? Growing evidence suggests it plays a role (2000)

High triglyceride levels are associated with several risk factors that substantially increase the risk of CAD. The metabolic syndrome is a constellation of signs and symptoms (e.g., postprandial hypertriglyceridemia, low LDL cholesterol levels, insulin resistance) that has been linked to a high incidence of heart disease. Treatment of hypertriglyceridemia begins with an aggressive lifestyle modification program. Dietary restriction of alcohol and carbohydrates can significantly lower triglyceride levels in many patients. Pharmacotherapy should be considered for patients at high risk of cardiac disease.
Study of the effects of austenitizing and tempering heat treatments on the alloy HT-9 (1982)

This paper investigates the potential use of the ferritic alloy Sandvik HT-9 (12 Cr - 1 Mo) as an alternative to stainless steels used in high-neutron-fluence environments. The neutron radiation influences embrittlement where the impact-energy versus test-temperature curve is seen displaced to the right. As a result, commercially effective solutioning and tempering processes are needed to suppress this effect in the pre-irradiated condition. The effects of austenitizing treatments on the impact energy of HT-9 were identified. 18 figures, 6 tables. (DLC)
Case-Control Study of Fetal Microchimerism and Breast Cancer (2008)

Results suggest that the enigma of why some parous women are not afforded protection from breast cancer by pregnancy might in part be explained by differences in FMc. Mechanistic studies of FMc-derived protection against breast cancer are warranted.
Engineering disulfide-linked single-chain Fv dimers [(sFv')<sub>2</sub>] with improved solution and targeting properties: anti-digoxin 26–10 (sFv')<sub>2</sub> and anti-c-erbB-2 741F8 (sFv')<sub>2</sub> made by protein folding and bonded through C-terminal cysteinyl peptides (1995)

Single-chain Fv fusions with C-terminal cysteinyl peptides (sFv') have been engineered using model sFv proteins based upon the 26-10 anti-digoxin IgG and 741F8 anti-c-erbB-2 IgG monoclonal antibodies. As part of the 741F8 sFv construction process, the PCR-amplified 741F8 VH gene was modified in an effort to correct possible primer-induced errors. Genetic replacement of the N-terminal beta-strand sequence of 741F8 VH with that from the FR1 of anti-c-erbB-2 520C9 VH resulted in a dramatic improvement of sFv folding yields. Folding in urea-glutathione redox buffers produced active sFv' with a protected C-terminal sulfhydryl, presumably as the mixed disulfide with glutathione. Disulfide-bonded (sFv')2 homodimers were made by disulfide interchange or oxidation after reductive elimination of the blocking group. Both 26-10 (sFv')2 and 741F8 (sFv')2 existed as stable dimers that were well behaved in solution, whereas 741F8 sFv and sFv' exhibited considerable self-association. The 741F8 sFv binds to the extracellular domain (ECD) of the c-erbB-2 oncogene protein, which is often overexpressed in breast cancer and other adenocarcinomas. The recombinant ECD was prepared to facilitate the analysis of 741F8 binding site properties; the cloned ECD gene, modified to encode a C-terminal Ser-Gly-His6 peptide, was transfected into Chinese hamster ovary cells using a vector that also expressed dihydrofolate reductase to facilitate methotrexate amplification. Optimized cell lines expressed ECD-His6 at high levels in a cell bioreactor; after isolation by immobilized metal affinity chromatography, final ECD yields were as high as 47 mg/l. An animal tumor model complemented physicochemical studies of 741F8 species and indicated increased tumor localization of the targeted 741F8 (sFv')2 over other monovalent 741F8 species.