gebhart/patrepeval huggingface dataset:
Analysis of Synthesis, Stability, Phosphorylation, and Interacting Polypeptides of the 34-Kilodalton Product of Open Reading Frame 6 of the Early Region 4 Protein of Human Adenovirus Type 5 (1999)

The 34-kDa early-region 4 open reading frame 6 (E4orf6) product of human adenovirus type 5 forms complexes with both the cellular tumor suppressor p53 and the viral E1B 55-kDa protein (E1B-55kDa). E4orf6 can inhibit p53 transactivation activity, as can E1B-55kDa, and in combination these viral proteins cause the rapid turnover of p53. In addition, E4orf6-55kDa complexes play a critical role at later times in the regulation of viral mRNA transport and shutoff of host cell protein synthesis. In the present study, we have further characterized some of the biological properties of E4orf6. Analysis of extracts from infected cells by Western blotting indicated that E4orf6, like E1A and E1B products, is present at high levels until very late times, suggesting that it is available to act throughout the infectious cycle. This pattern is similar to that of E4orf4 but differs markedly from that of another E4 product, E4orf6/7, which is present only transiently. Synthesis of E4orf6 is maximal at early stages but ceases completely with the onset of shutoff of host protein synthesis; however, it was found that unlike E4orf6/7, E4orf6 is very stable, thus allowing high levels to be maintained even at late times. E4orf6 was shown to be phosphorylated at low levels. Coimmunoprecipitation studies in cells lacking p53 indicated that E4orf6 interacts with a number of other proteins. Five of these were shown to be viral or virally induced proteins ranging in size from 102 to 27 kDa, including E1B-55kDa. One such species, of 72 kDa, was shown not to represent the E2 DNA-binding protein and thus remains to be identified. Another appeared to be the L4 100-kDa nonstructural adenovirus late product, but it appeared to be present nonspecifically and not as part of an E4orf6 complex. Apart from p53, three additional cellular proteins, of 84, 19, and 14 kDa were detected by using an adenovirus vector that expresses only E4orf6. The 19-kDa species and a 16-kDa cellular protein were also shown to interact with E4orf6/7. It is possible that complex formation with these viral and cellular proteins plays a role in one or more of the biological activities associated with E4orf6 and E4orf6/7.
Dye free automated cell counting and analysis (2012)

We have developed an automated cell counting method that uses images obtained at multiple focal heights to enumerate cells in confluent culture. By taking the derivative of image intensity with respect to focal height using two complementary images, we are able to count high-density monolayers of cells over a large image area. Our method resists errors arising from variability in the focal plane caused by flatness or tilt non-uniformities with a minimal amount of focal plane alignment, allowing the automated collection of images across a large area.
SPEC Process III. Synthesis of a Macroporous Silica-Based Crown Ether-Impregnated Polymeric Composite Modified with 1-Octanol and its Adsorption Capacity for Sr(II) Ions and Some Typical Co-Existent Metal Ions (2008)

A macroporous silica-based 4,4′,(5′)-di(t-butylcyclohexano)-18-crown-6 (DtBuCH18C6)-impregnated polymeric composite, (DtBuCH18C6 + Oct)/SiO 2 -P, was synthesized by molecular modification of DtBuCH18C6 with 1-octanol through hydrogen bonding. This was achieved by impregnating and immobilizing DtBuCH18C6 and 1-octanol molecules in the pores of macroporous SiO 2 -P particles possessing a mean pore diameter of 50 μm. The adsorption of some fission and non-fission products contained in highly active liquid waste (HLW) such as Ru(III), Mo(VI), Pd(II), Na(I), K(I), Cs(I), Sr(II), Ba(II), La(III) and Y(III) onto (DtBuCH18C6 + Oct)/SiO 2 -P was investigated at 298 K. The effects of contact time and HNO 3 concentration within the range 0.1–5.0 M were examined. Increasing the HNO 3 concentration from 0.1 M to 2.0 M led to a significant increase in the adsorption of Sr(II), followed by a decrease when the HNO 3 concentration was increased further to 5.0 M. The optimum concentration of HNO 3 in the adsorption of Sr(II) onto (DtBuCH18C6 + Oct)/SiO 2 -P was determined as 2.0 M. For the other ions examined with the exception of Ba(II), very weak or almost non-existent adsorption capacities were observed at all HNO 3 concentrations. The leaching of total organic carbon (TOC) from (DtBuCH18C6 + Oct)/SiO 2 -P into the aqueous phase was evaluated. Over the HNO 3 concentration range 0.1–3.0 M, the quantity of TOC leached was ca. 40 ppm compared with 355.8–373.3 ppm at HNO 3 concentrations above 4.0 M. Significant reduction of TOC leaching from the macroporous silica-based DtBuCH18C6-impregnated polymeric composite was achieved in 2.0 M HNO 3 . Such behaviour could be of great benefit in using (DtBuCH18C6 + Oct)/SiO 2 -P for the partitioning of Sr(II) from HLW by extraction chromatography.
Response to Comment on “Arsenic Removal Using Mesoporous Alumina Prepared via a Templating Method” (2004)

ADVERTISEMENT RETURN TO ISSUEPREVCorrespondence/Rebut...Correspondence/RebuttalResponse to Comment on "Arsenic Removal Using Mesoporous Alumina Prepared via a Templating Method"Younghun Kim, Changmook Kim, Inhee Choi, Selvaraj Rengaraj, and Jongheop YiView Author Information School of Chemical Engineering Seoul National University San 56-1, Shillim-dong, Kwanak-gu Seoul 151-742, KoreaCite this: Environ. Sci. Technol. 2004, 38, 11, 3216Publication Date (Web):April 27, 2004Publication History Published online27 April 2004Published inissue 1 June 2004https://pubs.acs.org/doi/10.1021/es0403864https://doi.org/10.1021/es0403864article-commentaryACS PublicationsCopyright © 2004 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views322Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (15 KB) Get e-AlertscloseSUBJECTS:Adsorption,Arsenic,Kinetics,Oxides,Sorption Get e-Alerts
Characterisation of coplanar waveguideon epitaxial layers (1997)

The effect of thin AlInAs/GaInAs epitaxial layers on the propagation of electrical signals in coplanar waveguide transmission lines fabricated on semi-insulating indium phosphide substrates is examined. It is shown that argon isolation implants effectively reduce conduction losses in these layers to negligible levels at radio, microwave, and millimetre-wave frequencies.
Fuzzy Optimization Techniques Applied to the Design of a Digital PMSM Servo Drive (2004)

This paper presents a novel design approach by applying gradient optimization with fuzzy step-sizing techniques to the design of a digital permanent magnet synchronous motor (PMSM) servo drive. The servo specifications and design variables are specified and analyzed to formulate a controller optimization problem. The servo responses are then fed back to evaluate the overall system performances, which can be expressed as objective functions with respect to the servo control parameters. According to the objective functions and design specifications, the servo control parameters can be properly tuned toward their optimal values by using the proposed optimization techniques. In order to improve the convergent rate of the optimization process, a fuzzy-logic based step-size tuning strategy is presented. Because of the nonlinear property of the digital servo drives, the tuned servo control parameters may be only optimal for a particular operating point, therefore, once the optimum design is achieved, the proposed fuzzy optimizing controller can perform as an intelligent tuner for on-line gain adaptation under different loading conditions. The proposed fuzzy optimization servo tuner has been realized under a PC-MATLAB-based environment with an on-line controlled digital PMSM servo drive. Simulation and experimental results indicate that the control parameters of a digital PMSM servo drive can be optimized for its dynamic responses under various load conditions.
An efficient sign prediction method for DCT coefficients and its application to reversible data embedding in scrambled JPEG image (2013)

In this paper, an efficient DCT sign prediction method is proposed. Unlike the conventional methods that depend on information from both spatial and frequency domains, the proposed method operates solely in the frequency domain by exploiting the pixel value patterns represented by the corresponding DCT basis vectors. In particular, each block is classified into five categories, namely, complex-pattern, complex-nonpattern, simple-smooth, simple-pattern and simple-texture, and each is treated differently using the proposed predictor. The proposed sign prediction method is then applied to realize reversible data embedding using sign information in a scrambled JPEG compressed image. This work is the first of its kind in using sign information for data embedding purposes. Basic performance of the proposed sign prediction and the proposed reversible data embedding method in scrambled JPEG image are verified using standard test images.
HIGHWAY PLANNING, WITH PARTICULAR REFERENCE TO TRAFFIC CAPACITIES. ROAD ENGINEERING DIVISION. (1946)

Keywords TRAFFIC, GROWTH, FORECASTS, ROADS, CAPACITY, SPEED, LIMITS, INTERSECTIONS, RATIOS, DESIGN, PLANNING, HIGHWAYS, WIDTH, CALCULATIONS, VEHICLES, CARS, MOTORCYCLES, LANES, PARKING, SIGNALS, ROUNDABOUTS, COSTS, PEAK HOURS UK, USA... Show All
Nectin-like proteins mediate axon–Schwann cell interactions along the internode and are essential for myelination (2007)

Axon-glial interactions are critical for the induction of myelination and the domain organization of myelinated fibers. Although molecular complexes that mediate these interactions in the nodal region are known, their counterparts along the internode are poorly defined. We report that neurons and Schwann cells express distinct sets of nectin-like (Necl) proteins: axons highly express Necl-1 and -2, whereas Schwann cells express Necl-4 and lower amounts of Necl-2. These proteins are strikingly localized to the internode, where Necl-1 and -2 on the axon are directly apposed by Necl-4 on the Schwann cell; all three proteins are also enriched at Schmidt-Lanterman incisures. Binding experiments demonstrate that the Necl proteins preferentially mediate heterophilic rather than homophilic interactions. In particular, Necl-1 on axons binds specifically to Necl-4 on Schwann cells. Knockdown of Necl-4 by short hairpin RNA inhibits Schwann cell differentiation and subsequent myelination in cocultures. These results demonstrate a key role for Necl-4 in initiating peripheral nervous system myelination and implicate the Necl proteins as mediators of axo-glial interactions along the internode.
Surgical Treatment of Erectile Dysfunction (2012)

Erectile dysfunction (ED) is defined as the consistent or recurrent inability of a man to attain and/or maintain penile erection sufficient for sexual activity. According to current guidelines, a 3-month symptom duration is accepted to establish the diagnosis except for some cases of trauma or surgically induced ED (Lewis R 2010). ED affects physical and psychosocial health and has a significant impact on the quality of life (QoL) of sufferers and their partners and families. Epidemiologic studies for ED estimate the prevalence of ED ranging between 20-30 % among men aged between 40-80 years (Laumannn et al 2005, Lewis R 2010). These differences in reported incidences are probably due to variations in the methodology and the age and socioeconomic status of the study populations.
A Curcumin Analog Exhibits Multiple Biologic Effects on the Pathogenesis of Alzheimer’s Disease and Improves Behavior, Inflammation, and β-Amyloid Accumulation in a Mouse Model (2020)

Drugs for the treatment of Alzheimer's disease (AD) are in urgent demand due to the unmet need and the social burden associated with the disease. Curcumin has been historically considered as a beneficial product for anti-aging and AD. However, many efforts to develop curcumin for clinical use are hindered mainly due to its poor bioavailability. Recent development in drug delivery and structural design has resolved these issues. In this study, we identified a small molecule, TML-6, as a potential drug candidate for AD through screening a panel of curcumin derivatives using six biomarker platforms related to aging biology and AD pathogenesis. The structural modification of TML-6 is designed to improve the stability and metabolism of curcumin. Cell biological studies demonstrated that TML-6 could inhibit the synthesis of the β-amyloid precursor protein and β-amyloid (Aβ), upregulate Apo E, suppress NF-κB and mTOR, and increase the activity of the anti-oxidative <i>Nrf2</i> gene. In the 3x-Tg AD animal model, TML-6 treatment resulted in significant improvement in learning, suppression of the microglial activation marker Iba-1, and reduction in Aβ in the brain. Although TML-6 exhibited a greater improvement in bioavailability as compared to curcumin, formulation optimization and toxicological studies are under development to assure its druggability. Taken together, TML-6 meets the current strategy to develop therapeutics for AD, targeting the combination of the Aβ cascade and aging-related biology processes.
The Electrochemical Behavior and Passivity of Titanium (1959)

In the active condition, corrosion of titanium is controlled by activation polarization. The metal presents a surface with a low exchange current for a variety of electrochemical reactions. Low exchange currents are not conducive to establishment of passivity. However, this is overcome by unusually active critical potentials for passivity. This accounts for the ease with which titanium is passivated by many substances which are only slightly oxidizing. It also explains the ease with which the metal can be protected anodically by galvanic coupling to other metals and alloys. Anodic deposition of thick titanium oxide films is observed under some experimental conditions.
Parallel-optical-interconnecting multiwavelength star network (POIMS Net) for the ultimate in high-capacity switching (2002)

POIMS Net is a passive optical network that combines time-, space-, and wavelength-division techniques to field network capacities far beyond 100 Gb/s. We describe its concept, practical implementation with an arrayed-waveguide-grating router, and application to the ATM switching fabric.
Virulence properties and attaching-effacing activity of Escherichia coli O45 from swine postweaning diarrhea (1994)

Escherichia coli O45 isolates associated with swine postweaning diarrhea in Québec were characterized with respect to virulence determinants genetically and investigated for their attaching and effacing (A/E) activities by experimental inoculation of gnotobiotic piglets and by the HEp-2 cell adherence assay. All of 32 isolates tested were negative for enterotoxigenic and verotoxigenic E. coli virulence determinants, heat-labile enterotoxin (LT), heat-stable enterotoxins (STap, STb), verotoxins (VT1, VT2), and F4 (K88), F5 (K99), F6 (987P), and F41, except one STb-positive and two F4-positive isolates. A total of 25 isolates hybridized with an EaeA probe, and 11 hybridized with an enteropathogenic E. coli adherence factor (EAF) probe. None of 32 isolates hybridized with a bundle-forming pilus (BFP) probe. The EAF, EaeA, and BFP factors have been associated with human enteropathogenic E. coli strains. A total of 10 of 12 eaeA-positive porcine O45 isolates induced A/E lesions characterized by intimate adherence of bacteria to the intestinal epithelial cell membrane with effacement of the microvilli, similar to those of human attaching-effacing E. coli. However, A/E lesions were not observed in the piglets inoculated with any one of three eaeA-negative O45 isolates. All E. coli O45 isolates were non-adherent to HEp-2 cells. Thus, we have demonstrated the production of typical A/E lesions by nonenterotoxigenic E. coli O45 isolates from swine postweaning diarrhea. The results indicate the significance of the eaeA gene in A/E activities of these isolates and suggest that EAF and BFP are not involved in O45 E. coli infection of weaning piglets.
Relation extraction between bacteria and biotopes from biomedical texts with attention mechanisms and domain-specific contextual representations (2019)

We demonstrated that our proposed contributions to this task can be used to extract rich lexical, syntactic, and semantic features that effectively boost the model's performance. Moreover, we analyzed the trade-off between precision and recall to choose the proper cut-off to use in real-world applications.
Uncoupling of the proliferation and differentiation signals mediated by the murine macrophage colony-stimulating factor receptor expressed in myeloid FDC-P1 cells. (1995)

The macrophage colony-stimulating factor (M-CSF) regulates proliferation and differentiation of cells belonging to the monocytic lineage. We have investigated the nature and origin of the proliferation and differentiation signals derived from the M-CSF receptor (Fms) by mutating Fms at the four tyrosine autophosphorylation sites and examining their biological effects in an FDC-P1 clone. Wild-type Fms stimulated both growth and differentiation of FDC-P1 cells in response to M-CSF stimulation. In contrast, both proliferation and differentiation were differentially disrupted by mutations affecting the four tyrosine autophosphorylation sites. These analyses revealed that: (a) none of the four autophosphorylation sites studied (Y697, Y706, Y721, and Y807) were essential for M-CSF-dependent proliferation of the FDC-P1 clone; (b) Y697, Y706, and Y721 sites, located in the kinase insert region of Fms, were not necessary for differentiation, but their presence augmented this process; (c) mutation of the Y807 site totally abrogated the differentiation of the FDC-P1 clone and simultaneously increased the rate of M-CSF-dependent proliferation; and (d) conversely, increasing the intracellular cAMP level blocked the growth signal in the FDC-P1 clone but had no effect on differentiation. These results suggest that autophosphorylation of Fms at the Y807 site controls the balance between signals for growth and differentiation.
Characteristic analysis of paper feeding with rubber-covered roller (2004)

Paper feeding problems find technical applications in numerous information equipments. For example, copy machine, printer, automatic teller machine and etc.. This paper deals with the features of paper feeding as a form of frictional drives and the two schemes in finite element analysis of paper transport velocity with a pair of a rubber-covered roller and a rigid roller.; One is an incremental theory explaining transitional behaviors of frictional drive from slipping between a rubber covered roller and a paper to stationary tractive rolling step by step of revolution.. The other is a direct solution using by the Galilean transform converting the governing equations of motion to a spatially dependent form, which is applicable to viscoelastic and inertial effects.
Effect of variable breath-hold positions during cardiac magnetic resonance on measures of left ventricular mechanics (2014)

Background Measures of left ventricular cardiac mechanics such as strains and torsion are becoming increasingly important for assessing heart function. Cardiac magnetic resonance (CMR) can be used to quantify cardiac mechanics using several methods such as tagged CMR or cine Displacement Encoding with Stimulated Echoes (DENSE). These images are generally acquired during an end-expiratory breath-hold. Unfortunately, it is difficult for subjects to hold their breath at the exact same position when undergoing a series of breath-holds during a typical CMR study. For example, end-expiratory breath-hold positions have an average range of about 8 millimeters (mm). The effects of different breath-hold positions on measures of cardiac mechanics have not been investigated. We hypothesized that the normal variability in breath-hold positions would significantly affect the quantification of left ventricular strains and torsion.
Osmia Spp. Reared in Artificial Nesting Sites in a Backyard Environment (1999)

Controlled pollination in field cages is used at the United States Department of Agriculture - Agricultural Research Service North Central Regional Plane Introduction Station (NCRPIS) for seed increase of several plane species. Honey bees, Apis mellifera L., have been used almost exclusively for several years. Recently we began investigating other pollinating insects for controlled pollination. 0smia cornifrons (Radoszkowski), a solitary bee imported from Japan, has been an excellent early-season pollinator. We placed domiciles of these bees in backyards of the NCRPIS staff to aid us in providing adequate numbers of bees for use in field cages the following growing season. We used an X-ray technique to aid in counting the number of bees present in rearing straws. We also note some of the different planes which the bees visited for food.
Driver somatic mutations identify distinct disease entities within myeloid neoplasms with myelodysplasia (2014)

Our knowledge of the genetic basis of myelodysplastic syndromes (MDS) and myelodysplastic/myeloproliferative neoplasms (MDS/MPN) has considerably improved. To define genotype/phenotype relationships of clinical relevance, we studied 308 patients with MDS, MDS/MPN, or acute myeloid leukemia evolving from MDS. Unsupervised statistical analysis, including the World Health Organization classification criteria and somatic mutations, showed that MDS associated with SF3B1-mutation (51 of 245 patients, 20.8%) is a distinct nosologic entity irrespective of current morphologic classification criteria. Conversely, MDS with ring sideroblasts with nonmutated SF3B1 segregated in different clusters with other MDS subtypes. Mutations of genes involved in DNA methylation, splicing factors other than SF3B1, and genes of the RAS pathway and cohesin complex were independently associated with multilineage dysplasia and identified a distinct subset (51 of 245 patients, 20.8%). No recurrent mutation pattern correlated with unilineage dysplasia without ring sideroblasts. Irrespective of driver somatic mutations, a threshold of 5% bone marrow blasts retained a significant discriminant value for identifying cases with clonal evolution. Comutation of TET2 and SRSF2 was highly predictive of a myeloid neoplasm characterized by myelodysplasia and monocytosis, including but not limited to, chronic myelomonocytic leukemia. These results serve as a proof of concept that a molecular classification of myeloid neoplasms is feasible.