gebhart/patrepeval huggingface dataset:
Knee kinematics and kinetics during gait, step and hop in males with a 16 years old ACL injury compared with matched controls (2006)

The objective of this study was to compare subjects who sustained an ACL injury during soccer 16 years ago with a control group with regard to knee kinematics and kinetics of gait, step activity and cross over hop. Secondly, in the injured subjects, the purpose was to study the impact on kinematics and kinetics of characteristics such as operative status, meniscal resection, being symptomatic, having knee extensor weakness and of having radiographic knee OA. Data from a 3-dimensional gait analysis system (VICON) were used to calculate kinetics and kinematics during gait, step activity and cross over hop of 12 male subjects who had an anterior cruciate ligament injury 16 years earlier. Twelve uninjured subjects matched for age, sex, BMI and activity level served as controls. No significant differences in knee kinematics and kinetics between the ACL group and the control group were found. The variability of some parameters of step and all parameters of hop activity was generally larger in the ACL injured subjects compared with the controls. The ACL injured subjects had a significantly worse clinical status compared with the controls. Reduced knee extension strength was associated with joint moment reductions especially during step activity and cross over hop. Despite a significantly worse clinical status, as determined by self-report and isokinetic strength testing, no significant differences were seen in knee joint kinetics and kinematics in an ACL injured group 16 years after injury compared with a matched control group. The variation was larger among the ACL injured subjects indicating the need for larger study groups in gait and movement analysis in long-term follow-up of ACL injury.
Non-redundant error correction of uncoded M-DPSK using a modified Viterbi algorithm and a sliding block non-coherent multi-symbol detector (2002)

Differential phase shift keying (DPSK) is often used in applications where system requirements make a phase-coherent reference at the receiver difficult to obtain or economically unattractive. Previous methods of reducing the energy penalty associated with the lack of phase coherence in DPSK using non-coherent detection have focused on non-redundant error correction (NEC) or sequence estimation techniques, optimal non-coherent (block) detection, trellis code modulation (TCM), and combinations of these methods. In the present paper, a new and general sub-optimal non-redundant error correction method for non-coherent detection of M-DPSK is proposed. The new method decides from 1 to N DPSK data symbols using a sliding block of N+1 phase measurements without adding any redundancy to the data signal using a technique derived from the Viterbi decoder for TCM. Simulation results for the additive white Gaussian noise environment show that while retaining the same general structure for different PSK signaling constellations, the new method achieves much of the performance of NEC and block detection without the exponential complexity in block length and TCM without any coding or increase in the signal constellation space.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Synthesis of Four Lysine-Linked Cereulide Analogues Showing Ionophoric Activity Towards Potassium Cations as Lead Compounds for Emetic Toxin-Detection by Immunoassays (2009)

An improved total synthesis of the emetic toxin cereulide and the preparation of four lysine-linked cereulide analogues is described. The cereulide analogues are prepared by replacing one of the amino acid residues in cereulide with lysine. The cereulide analogues demonstrate ionophoric activity towards alkali metal ions and inorganic ammonium ions, and are currently being used to develop an enzyme-linked immunosorbent assay for cereulide.
Mimicry of a neutralizing epitope of the major outer membrane protein of Chlamydia trachomatis by anti-idiotypic antibodies (1994)

The major outer membrane protein (MOMP) is a primary target antigen for the development of chlamydial vaccine. This protein is composed of four variable domains (I to IV) flanked by constant regions. Some of the variable domains contain antigenic determinants that elicit a neutralizing antibody response. Murine monoclonal antibodies (MAbs) against three nonoverlapping epitopes of MOMP were developed. One of these, called DP10, bound to all serovars, as shown by immunoblot analysis, and neutralized chlamydial infectivity for hamster kidney (HaK) cells in a complement-independent in vitro assay. Furthermore, analysis of the fine specificity of this MAb showed that it recognized a synthetic peptide contained within variable domain IV of the MOMP. Anti-idiotypic antibodies (aId) directed against this anti-MOMP MAb were produced in rabbits. These aId specifically bound to the relevant idiotype (DP10) and inhibited the binding of anti-MOMP MAb (DP10) to MOMP preparations in a dose-dependent fashion. The specificity of our aId for the binding site of anti-MOMP MAb is further suggested by the binding inhibition of affinity-purified aId to DP10 by the synthetic peptide defined by the idiotype. In addition, these aId also reacted with anti-MOMP antisera from rats and mice, suggesting an idiotypic cross-reactivity between these species. Finally, immunization of naive mice with aId induced an antibody response directed against the peptide defined by our anti-MOMP MAb and with neutralizing activity. Taken together, these data suggest that aId mimic a neutralization site on MOMP and could serve as a surrogate antigen to induce protective immunity against Chlamydia trachomatis.
[Study on monoclonal antibodies against porcine serum E receptor]. (1993)

Three hybridoma cell lines producing monoclonal antibodies (McAbs) against porcine serum E receptor (PSER) were established by fusing mouse myeloma SP2/o cells with spleen cells of BALB/c mouse immunized with PSER. The ascites McAbs were purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. The McAbs were found to belong to the IgG1 and IgG2b subclasses, respectively. These McAbs inhibited reverse E-rosette formation and enhanced or depressed lymphocyte proliferation induced by PHA or ConA. The specificity of these McAbs was proved by immuno-dot blot and Western blot assay. The potential application of these McAbs in the study of structure and biological function of the E-receptor is discussed.
In Vitro Evaluation of Experimental Agents for Anti‐<scp>HIV</scp> Activity (1993)

This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.
Complications of foldable intraocular lenses requiring explantation or secondary intervention—2007 survey update (2008)

The tenth annual survey of complications associated with foldable intraocular lenses (IOLs) requiring explantation or secondary intervention was sent to members of the American Society of Cataract and Refractive Surgery and the European Society of Cataract and Refractive Surgeons. Preoperative data about visual acuity, patient signs and symptoms, and complications requiring IOL removal were evaluated. Complications were then tabulated for each of the following major foldable IOL groups: 1-piece (plate) silicone, 1-piece hydrophobic acrylic with haptics, 3-piece silicone, 3-piece hydrophobic acrylic, 3-piece hydrophilic acrylic (hydrogel), 3-piece unknown, multifocal acrylic, and multifocal silicone. One hundred forty-two surveys were returned for evaluation. Dislocation/decentration, incorrect IOL power, glare/optical aberrations, and IOL calcification were the most common reasons for removing foldable IOLs. Good surgical technique, accurate IOL power measurements, and high manufacturing standards for foldable IOL materials and designs are the most important factors in preventing complications.
Molecular Cloning and Characterization of a Mouse Oviduct-Specific Glycoprotein1 (1995)

In the present study, we have isolated the cDNA for the mouse oviduct-specific glycoprotein (MOGP) by screening the mouse oviduct cDNA library with the bovine oviduct-specific glycoprotein (BOGP)-cDNA probe and by the 5' rapid amplification of the cDNA end (5'RACE). The total length of cDNA was determined to be 2525 base pairs (bp) by sequence analysis. The coding region contained 2163 bp translating to 721 amino acids. Based on comparisons with the N-terminal amino acid sequences of purified-BOGP and of hamster oviduct-specific glycoprotein (oviductin), it was inferred that the derived amino acid sequence contained a signal peptide region of 21 amino acids and a mature MOGP (core protein) region of 700 amino acids (76,515 daltons). It was also inferred that the mature MOGP contained three potential N-linked glycosylation sites and 24 possible O-linked glycosylation sites, and had the unique seven-residue repeat sequence (21 repeats) within the predicted sequence in the C-terminal side. The amino acid sequence of a portion of MOGP was highly homologous to that of BOGP (71% identity), baboon oviduct-specific glycoprotein (61% identity), and human oviduct-specific glycoprotein (77% identity). Significant homologies were also observed with two mammalian secretory proteins that were reported as a mammalian member of a chitinase protein family. Northern blot hybridization with a DIG-labeled probe indicated that a single message of 2.8 kb was present in total RNA prepared from oviductal tissue. In situ hybridization using MOGP-cDNA showed that a MOGP message was only detected in the oviductal epithelial cells. These results strongly suggest that a significant degree of homology exists among oviduct-specific glycoproteins of various mammalian species.
Early Upregulation of Acute Respiratory Distress Syndrome-Associated Cytokines Promotes Lethal Disease in an Aged-Mouse Model of Severe Acute Respiratory Syndrome Coronavirus Infection (2009)

Several respiratory viruses, including influenza virus and severe acute respiratory syndrome coronavirus (SARS-CoV), produce more severe disease in the elderly, yet the molecular mechanisms governing age-related susceptibility remain poorly studied. Advanced age was significantly associated with increased SARS-related deaths, primarily due to the onset of early- and late-stage acute respiratory distress syndrome (ARDS) and pulmonary fibrosis. Infection of aged, but not young, mice with recombinant viruses bearing spike glycoproteins derived from early human or palm civet isolates resulted in death accompanied by pathological changes associated with ARDS. In aged mice, a greater number of differentially expressed genes were observed than in young mice, whose responses were significantly delayed. Differences between lethal and nonlethal virus phenotypes in aged mice could be attributed to differences in host response kinetics rather than virus kinetics. SARS-CoV infection induced a range of interferon, cytokine, and pulmonary wound-healing genes, as well as several genes associated with the onset of ARDS. Mice that died also showed unique transcriptional profiles of immune response, apoptosis, cell cycle control, and stress. Cytokines associated with ARDS were significantly upregulated in animals experiencing lung pathology and lethal disease, while the same animals experienced downregulation of the ACE2 receptor. These data suggest that the magnitude and kinetics of a disproportionately strong host innate immune response contributed to severe respiratory stress and lethality. Although the molecular mechanisms governing ARDS pathophysiology remain unknown in aged animals, these studies reveal a strategy for dissecting the genetic pathways by which SARS-CoV infection induces changes in the host response, leading to death.
Erlang capacity of a power controlled integrated voice and data CDMA system (2002)

In a previous paper by A.M. Viterbi and A.J. Viterbi (see IEEE J. Sel. Areas Commun., vol.11, no.6, p.892-900, 1993), an analytical approach to computing the Erlang capacity of a voice-only CDMA system was presented. Capacity was defined as the maximum Erlangs of voice traffic that can be supported while limiting the total received interference and noise below a threshold, 99% of the time. In this paper, we evaluate the Erlang capacity of a CDMA system with voice and data users. The outage condition is first related to power control feasibility. It is shown that the outage condition is more stringent and hence ensures power control feasibility. The outage condition is then extended to the case of a CDMA system with voice and data users. An approximate analysis that takes into account imperfect power control, activity factors and different quality requirements is presented. Simulations are used to validate the approximate analysis.
Fluorescence-Raman Dual Modal Endoscopic System for Multiplexed Molecular Diagnostics (2015)

Optical endoscopic imaging, which was recently equipped with bioluminescence, fluorescence, and Raman scattering, allows minimally invasive real-time detection of pathologies on the surface of hollow organs. To characterize pathologic lesions in a multiplexed way, we developed a dual modal fluorescence-Raman endomicroscopic system (FRES), which used fluorescence and surface-enhanced Raman scattering nanoprobes (F-SERS dots). Real-time, in vivo, and multiple target detection of a specific cancer was successful, based on the fast imaging capability of fluorescence signals and the multiplex capability of simultaneously detected SERS signals using an optical fiber bundle for intraoperative endoscopic system. Human epidermal growth factor receptor 2 (HER2) and epidermal growth factor receptor (EGFR) on the breast cancer xenografts in a mouse orthotopic model were successfully detected in a multiplexed way, illustrating the potential of FRES as a molecular diagnostic instrument that enables real-time tumor characterization of receptors during routine endoscopic procedures.
<title>Planar substrate surface plasmon resonance probe</title> (1996)

The theory and design of a new planar surface plasmon resonance probe are presented. The new sensor is based on a folded light pipe combined with a telecentric lens. The inexpensive sensing elements are constructed from pieces of microscope slide and are easy to manufacture. A prototype probe design capable of measuring 2 samples simultaneously was constructed. This is the first SPR probe capable of multiplexing and first order sensing. Initial experimental refractive index testing for 0% to 41% by weight glucose solutions exhibited a sensitivity of 3 by 10<SUP>-5</SUP> index of refraction units.
Instrumentation for Automated Molecular Marker Acquisition and Data Analysis (1996)

Developing molecular markers has become the most rapid method for constructing detailed genetic maps. This technology can be used to streamline breeding programs, to help identify specific individuals, and to quickly discern genetic variation in large populations. Since both breeding populations and genetic diversity tend to be relatively large in plants, the development and routine use of molecular genetic markers requires large numbers of samples to be processed. Thus, automation becomes a strong consideration. Automation should be thought of as modular. A modular approach to automation imparts the greatest flexibility for incremental improvements resulting from technological advances. Issues to consider when selecting among the different types of molecular marker assays available include: cost of developing new markers; cost of routine analysis; amount of information generated per marker; amenability of the assay technology to automation; and ease of incorporating markers into data bases of general utility (Figure 1 and Table 1).
The need for standardised broad scale bioassay testing: A case study using the red alga<i>Laurencia rigida</i> (1996)

Two major problems associated with biofouling studies are the lack of broad scale testing and failure to use consistent standards among different assays or studies. To address these issues the activity of two biologically active natural products, elatol and deschloroelatol, isolated from the marine red alga Laurencia rigida, and three commonly used biocides, Nopcocide N-96™, Irgarol 1051™ and Sea-Nine 211™, was compared, in a broad spectrum of bioassays. The activity of the different compounds varied substantially among different bioassay tests. Elatol and deschloroelatol had a narrow range of activity with strongest effects against invertebrate larvae. Both compounds were highly toxic. However, neither compound had strong activity against marine bacteria or the common epiphyte Ulva lactuca. Irgarol 1051 also had a narrow range of activity, only affecting algal settlement strongly. Nopcocide N-96 and Sea-Nine 211 had moderate to strong activity across the spectrum of bioassays, viz. growth of marine bacteria (Vibrio fischeri, Serratia sp.), inhibition of settlement of macroalgae (Ulva lactuca), toxicity (Balanus amphitrite), and inhibition of settlement of invertebrate larvae (Balanus amphitrite, Bugula neritina). Based on the results it is proposed that Sea-Nine 211, because of its broad spectrum activity, be used as a standard for comparative assessments of the antifouling activity of marine natural products and analogues.
Alveolina from Western Kutch-Systematics, Distribution and Stratigraphic Significance (1988)

Alveolina of Kutch Middle Eocene limestones and marls is identified as A. elliptica var. nuttalli . The variety is shown to have a characteristic shape (expressed as a ratio of polar and equatorial diameters), and more or less fixed number of flosculinised whorls; the variation in the development of non-flosculinised whor1s on the flosculinised ones is attributed to an ontogenetic development correlated with size increase. The variety shows a clear case of lateral facies variation, occurring in two 'apparent' horizons in deeper parts of the basin with vertical absence in between, but continuing throughout the rock unit in marginal shallower parts. The facies-governed nature of this larger benthic foraminiferal species and its presence in the Kutch Paleogene sequence without the earlier glomalveoline forms are considered important aspects in stratigraphic studies.
Effects of Blend Composition and Mixing Method on Mechanical and Morphological Properties of Zinc Dimethacrylate-Reinforced Acrylonitrile-Butadiene Copolymer Nanocomposites (2004)

Abstract Two kinds of rubber composites were prepared by melt mixing of acrylonitrile-butadiene rubber (NBR) with zinc dimethacrylate (ZMA). In one system ZMA was added in the form of powder; and, in another system, the ZMA was prepared by in-situ reaction of ZnO and methacrylic acid (MA) during melt mixing. Both systems showed similar tensile strength, however the elongation at break, tear strength, and fatigue resistance were higher for the in-situ prepared system. The composite prepared by direct addition of ZMA powder showed a corresponding peak for ZMA particles in the wide angle x-ray diffraction (WAXD) spectra, indicating the existence of two phases, while there was no such peak for the in-situ prepared blend. The comparison of cross-link densities of these two blends showed a higher proportion of total cross-link density (calculated from covalent and ionic cross-linking) for the blend prepared from the direct addition method. The morphology of torn surfaces based on field-emission scanning electron microscopy (FE-SEM), showed a different nature of fracture behavior in these blends. The mean particle size and distribution of ZMA in NBR matrix as measured by atomic force microscopy (AFM) and small angle x-ray scattering (SAXS) indicated the formation of nanostructured domains of ZMA of particle size in the range of 10–40 nm with more uniform distribution in the in-situ blends.
Quantum computing with distant single photon sources with insurance (2004)

We demonstrate the possibility to perform quantum computations using only single photon sources, linear optics elements and photon detectors. In contrast to common linear optics quantum computing proposals, the described scheme can be operated with insurance without relying on highly entangled ancilla photons. Universality is achieved by employing the properties of certain single photon sources, namely the fact that it is possible to encode the logical qubit within the state of a source as well as in the state of the generated photon. The proposed Ising gate allows to build cluster states for one-way quantum computing. Furthermore we describe the implementation of the quantum parity filter, enabling teleportation with insurance, and the generation of multiphoton entanglement on demand.
Interaction of LDL receptor‐related protein 4 (LRP4) with postsynaptic scaffold proteins via its C‐terminal PDZ domain‐binding motif, and its regulation by Ca<sup>2+</sup>/calmodulin‐dependent protein kinase II (2006)

We cloned here a full-length cDNA of Dem26[Tian et al. (1999)Mol. Brain Res., 72, 147-157], a member of the low-density lipoprotein (LDL) receptor gene family from the rat brain. We originally named the corresponding protein synaptic LDL receptor-related protein (synLRP) [Tian et al. (2002) Soc. Neurosci. Abstr., 28, 405] and have renamed it LRP4 to accord it systematic nomenclature (GenBank(TM) accession no. AB073317). LRP4 protein interacted with postsynaptic scaffold proteins such as postsynaptic density (PSD)-95 via its C-terminal tail sequence, and associated with N-methyl-D-aspartate (NMDA)-type glutamate receptor subunit. The mRNA of LRP4 was localized to dendrites, as well as somas, of neuronal cells, and the full-length protein of 250 kDa was highly concentrated in the brain and localized to various subcellular compartments in the brain, including synaptic fractions. Immunocytochemical study using cultured cortical neurons suggested surface localization in the neuronal cells both in somas and dendrites. Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) phosphorylated the C-terminal cytoplasmic region of LRP4 at Ser1887 and Ser1900, and the phosphorylation at the latter site suppressed the interaction of the protein with PSD-95 and synapse-associated protein 97 (SAP97). These findings suggest a postsynaptic role for LRP4, a putative endocytic multiligand receptor, and a mechanism in which CaMKII regulates PDZ-dependent protein-protein interactions and receptor dynamics.
Comment on the Proposed Suppression of Amphisbaena Dubia Rathke, 1863 (1962)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.