gebhart/patrepeval huggingface dataset:
Relevance of experimental paradigms of anesthesia induced neurotoxicity in the mouse (2019)

Routine general anesthesia is considered to be safe in healthy individuals. However, pre-clinical studies in mice, rats, and monkeys have repeatedly demonstrated that exposure to anesthetic agents during early post-natal periods can lead to acute neurotoxicity. More concerning, later-life defects in cognition, assessed by behavioral assays for learning and memory, have been reported. Although the potential for anesthetics to damage the neonatal brain is well-documented, the clinical significance of the pre-clinical models in which damage is induced remains quite unclear. Here, we systematically evaluate critical physiological parameters in post-natal day 7 neonatal mice exposed to 1.5% isoflurane for 2-4 hours, the most common anesthesia induced neurotoxicity paradigm in this animal model. We find that 2 or more hours of anesthesia exposure results in dramatic respiratory and metabolic changes that may limit interpretation of this paradigm to the clinical situation. Our data indicate that neonatal mouse models of AIN are not necessarily appropriate representations of human exposures.
Melatonergic Involvement in Diurnal Changes of Intraocular Pressure in Rabbit Eyes (1985)

Endogenous biochemical regulation of diurnal changes in intraocular pressure was investigated in rabbits. Various biochemical parameters in eye tissues, particularly the iris and ciliary body, were studied at peak (21:00 hr) and trough (09:00 hr) points of IOP. No statistical difference in choline acetyltransferase activity, adrenergic transmitter levels and dopamine concentration could be detected at these points. On the other hand, serotonin N-acetyltransferase activity was significantly higher at 21:00 hr (2.84 +/- 0.14 nmoles/mg protein/hr) than at 09:00 hr (2.18 +/- 0.16 nmoles/mg protein/hr) indicating that melatonin might be involved in the diurnal changes in IOP. Intracameral injections of various agents into rabbit eyes revealed that melatonin but not serotonin nor N-acetylserotonin raised IOP markedly, indicating that melatonin but not its precursors is involved in IOP regulation. Topical application of melatonin did not affect the IOP presumably because it does not cross the cornea effectively.
Purification and kinetic analysis of pea (Pisum sativum L.) NADPH:protochlorophyllide oxidoreductase expressed as a fusion with maltose-binding protein in Escherichia coli (1997)

NADPH:protochlorophyllide oxidoreductase (POR) catalyses the light-dependent reduction of protochlorophyllide to chlorophyllide, a key reaction in the chlorophyll biosynthetic pathway. To facilitate structure-function studies, POR from pea (Pisum sativum L.) has been overexpressed in Escherichia coli as a fusion with maltose-binding protein (MBP) at 5-10% of the total soluble cell protein. The fusion protein (MBP-POR) has been purified to greater than 90% homogeneity by a two-step affinity-purification procedure. This represents the first successful overexpression and purification of a plant POR. MBP-POR was found to be active, and the kinetic properties were determined using a continuous assay in which the rate of chlorophyllide formation was measured. The Vmax was 20.6+/-0.9 nmol.min-1.mg-1 and the Km values for NADPH and protochlorophyllide were 8.7+/-1.9 microM and 0.27+/-0.04 microM respectively. These results represent the first determination of the kinetic properties of a pure POR and the first report on the kinetics of POR from a dicotyledenous plant. The experiments described here demonstrate that the enzyme is not a 'suicide' enzyme, and the only components required for catalysis are NADPH, protochlorophyllide and light. Size-exclusion chromatography on a Superose 6 HR column indicated that MBP-POR has a molecular mass of 155 kDa (compared with the molecular mass of 80 kDa estimated by SDS/PAGE), indicating that it behaves as a dimer in solution. This is the first direct determination of the oligomerization state of POR.
HPLC determination of the cholesterol content of egg noodles as an indicator of egg solids (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHPLC determination of the cholesterol content of egg noodles as an indicator of egg solidsW. Jeffrey Hurst, M. D. Aleo, and Robert A. Martin Jr.Cite this: J. Agric. Food Chem. 1985, 33, 5, 820–822Publication Date (Print):September 1, 1985Publication History Published online1 May 2002Published inissue 1 September 1985https://pubs.acs.org/doi/10.1021/jf00065a014https://doi.org/10.1021/jf00065a014research-articleACS PublicationsRequest reuse permissionsArticle Views131Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Direct writing of optical gratings using a scanning electron microscope (1978)

The refractive-index change effect due to electron beam irradiation has been successfully applied to write directly optical gratings as large as 0.5 x 0.65 mm(2), with periods of 0.5-2 microm, in amorphous chalcogenide (for example, As(2)S(3), As(40)Se(10)S(40)Ge(10)) films, using a common scanning electron microscope. Fabrication techniques and optical characteristics are discussed.
158. The nutritive value of raw and pasteurized milk for mice (1937)

1. Experiments were carried out during the years 1931–5 in which mice on a basal diet of raw milk and white-flour biscuit were compared with mice on a similar diet except that the raw milk was replaced by commercially holderpasteurized (145–150° F. for 30 min.) milk belonging to the same batch. Each group of mice comprised in aggregate about 1100 animals, which were usually 4–6 weeks old at the commencement of observation. For reasons given on p. 206 no attempt was made to standardize the volume of milk consumed by the individual mice. Except in the preliminary experiments, when the milk was given in excess, the amount supplied was regulated more or less in accordance with the requirements of the average animal.
HSV vector-mediated gene delivery to the central nervous system. (2001)

The efficient and targeted transfer of genes is the goal of gene therapy. In the central nervous system (CNS), this is challenging due in part to the exquisite anatomy of the brain. Herpes simplex virus (HSV) vectors are particularly amenable to CNS therapies as they are capable of transducing a variety of cells, have a large transgene capacity and can exist as either oncolytic or non-immunogenic vectors. The versatility of this vector platform and its potential molecular therapeutic use in two CNS disorders, Alzheimer's disease and malignant brain tumors, will be discussed.
Accurate timed RTOS model for transaction level modeling (2010)

In this paper, we present an accurate timed RTOS model within transaction level models (TLMs). Our RTOS model, implemented on top of system level design language (SLDL), incorporates two key features: RTOS behavior model and RTOS overhead model. The RTOS behavior model provides dynamic scheduling, inter-process communication (IPC), and external communication for timing annotated user applications. While the RTOS behavior model is running, all RTOS events, such as context switch and interrupt handling, are passed to RTOS over-head model to adopt the overhead during system execution. Our RTOS overhead model has processor- and RTOS-specific pre-characterized overhead information to provide cycle approximate estimation. We demonstrate the applicability of our model using a multi-core platform executing a JPEG encoder. Experimental results show that the proposed RTOS model provides the high accuracy, 7% off compared to on-board measurements while simulating at speeds close to the reference C code.
Real-Time Bioluminometric Method for Detection of Nucleoside Diphosphate Kinase Activity (1999)

A real-time, simple and sensitive method for detection of nucleoside diphosphate (NDP) kinase activity has been developed. The assay is based on detection of ATP, generated in the NDP kinase reaction between a nucleoside triphosphate and adenosine diphosphate (ADP), by the firefly luciferase system. In the presence of 0.3 mM dGTP, the Km for ADP was found to be approximately 30 microM for the NDP kinase from Baker's yeast. In the presence of 250 microM ADP, the Km for dATP alpha S, dTTP alpha S, dGTP, dTTP, dCTP and GTP was found to be approximately 0.01, 0.03, 0.05, 0.25, 0.75 and 0.2 mM, respectively. The assay is sensitive and yields linear responses between 0.05-50 mU. The detection limit was found to be 0.05 mU of NDP kinase. The method was used to detect NDP kinase contamination in commercial enzyme preparations.
Inhibition of RNA-dependent DNA polymerase by thymidylate derivatives. (1972)

The effects of several thymidylate derivatives with reactive substituents on the RNA-dependent DNA polymerase of Rauscher murine leukemia virus were investigated. The reactive substituents include haloacetamido groups potentially capable of forming covalent bonds with the enzyme. The kinetics of inhibition with thymidine-3′,5′-di- m -bromoacetamidophenyl phosphate, the most active agent of the series, was competitive with poly(rA)·oligo(dT), the model primer-template used, and noncompetitive with the deoxynucleoside triphosphates. Preincubation of the virus with thymidine-3′,5′-di- m -bromoacetamidophenyl phosphate magnified the inhibition of DNA synthesis.

Inhibition of the reactions directed by DNA templates, such as salmon sperm DNA and poly[d(A-T)], was comparable to that found with poly(rA)·oligo(dT). The inhibition of tumor virus DNA polymerase is not selective, since we observed comparable inhibition of a DNA-dependent DNA-polymerase partly purified from phytohemagglutinin-stimulated normal human blood lymphocytes.
A Close Look at Proteins: Submolecular Resolution of Two- and Three-Dimensionally Folded Cytochrome c at Surfaces (2012)

Imaging of individual protein molecules at the single amino acid level has so far not been possible due to the incompatibility of proteins with the vacuum environment necessary for high-resolution scanning probe microscopy. Here we demonstrate electrospray ion beam deposition of selectively folded and unfolded cytochrome c protein ions on atomically defined solid surfaces in ultrahigh vacuum (10–10 mbar) and achieve unprecedented resolution with scanning tunneling microscopy. On the surface folded proteins are found to retain their three-dimensional structure. Unfolded proteins are observed as extended polymer strands displaying submolecular features with resolution at the amino acid level. On weakly interacting surfaces, unfolded proteins refold into flat, irregular patches composed of individual molecules. This suggests the possibility of two-dimensionally confined folding of peptides of an appropriate sequence into regular two-dimensional structures as a new approach toward functional molecular surface coatings.
Molecular analysis of a t(11;14)(q23;q11) from a patient with null‐cell acute lymphoblastic leukemia (1993)

/lp;&-3qChromosome 11, band q23, is the frequent site of recurring cytogenetic rearrangements in human leukemia. We have cloned and sequenced the breakpoint junctions from a patient who had null-cell acute lymphoblastic leukemia (ALL) with a t(11;14)(q23;q11). The chromosome 14 breakpoints occurred within the TCRD locus, close to two diversity segments. The chromosome 11 breakpoint occurred between two head-to-head heptamer sequences, and junctional diversity was evident at both derivative junctions, suggesting involvement of the V(D)J recombinase. The TCRA/D locus on the normal chromosome 14 had undergone a V delta 2-D delta 3-psi J alpha joining. Two phage clones with this VDJ rearrangement were isolated; one of these contained an intra-J alpha region deletion. Two clones with the derivative 11 junction were isolated; one of these had a similar, but not identical, deletion. A heptamer-nonamer recognition sequence (located approximately 70 kb 5' to C alpha), not associated with a TCR gene coding segment, was found in the immediate vicinity of both 5' breakpoints. We have designated this sequence 5'del for 5' deleting element. An intra-J alpha region deletion involving this heptamer-nonamer was previously identified in the leukemia cells recovered from a patient who had T-cell ALL. Fifty kilobases of DNA on 11q23 surrounding the breakpoint were cloned and analyzed. No CpG islands or conserved sequences were identified within this region.(ABSTRACT TRUNCATED AT 250 WORDS)
Augmented Reality Imaging System: 3D Viewing of a Breast Cancer. (2016)

The augmented reality system provided 3D visualization of the breast cancer with depth perception and visualization of the mass's spiculations. The augmented reality system should be further researched to determine the utility in clinical practice.
Fabrication of nanopillars by nanosphere lithography (2006)

A low cost nanosphere lithography method for patterning and generation of semiconductor nanostructures provides a potential alternative to the conventional top-down fabrication techniques. Forests of silicon pillars of sub-500 nm diameter and with an aspect ratio up to 10 were fabricated using a combination of the nanosphere lithography and deep reactive ion etching techniques. The nanosphere etch mask coated silicon substrates were etched using oxygen plasma and a time-multiplexed 'Bosch' process to produce nanopillars of different length, diameter and separation. Scanning electron microscopy data indicate that the silicon etch rates with the nanoscale etch masks decrease linearly with increasing aspect ratio of the resulting etch structures.
Lyme Disease and the Nervous System (1991)

Epidemiology and vectors Borrelia Burgdorfen pathogenesis and pathophysiology pathology general clinical features neurologic abnormalities in early Lyme disease neurologic abnormalities in late Lyme disease diagnosis of nervous system Lyme disease treatment prevention.
A single-instance incremental SAT formulation of proof- and counterexample-based abstraction (2010)

This paper presents an efficient, combined formulation of two widely used abstraction methods for bit-level verification: counterexample-based abstraction (CBA) and proof-based abstraction (PBA). Unlike previous work, this new method is formulated as a single, incremental SAT-problem, interleaving CBA and PBA to develop the abstraction in a bottom-up fashion. It is argued that the new method is simpler conceptually and implementation-wise than previous approaches. As an added bonus, proof-logging is not required for the PBA part, which allows for a wider set of SAT-solvers to be used.
Mechanism of emulsion polymerization of styrene using a reactive surfactant (2001)

Abstract The emulsion polymerization of styrene using the reactive surfactant sodium dodecyl allyl sulfosuccinate (TREM LF‐40) was studied. The polymerization kinetics were found to be unusual in that R p was not directly proportional to N p ( R p ∝ N p 0.67 ). Several reasons are stated to explain the unusual kinetics, including chain transfer to TREM LF‐40, copolymerization of styrene with TREM LF‐40, and the influence of the homopolymer of TREM LF‐40 [poly(TREM)] and/or the copolymer [poly(TREM‐ co ‐styrene)] on the entry and exit rates of free radicals. The possibility of both chain transfer and copolymerization exists primarily at the oil/water interface, whereas both can also occur in the aqueous and monomer phases. Bulk polymerizations of styrene in the presence of TREM LF‐40 and poly(TREM) were conducted, and the results show that the reaction rate decreased for the styrene/TREM LF‐40 system. Latex characterization by serum replacement and titration measurements provided evidence for the chemical bonding of TREM LF‐40 to the polymer particles. The fraction of chemically bound reactive surfactant decreased with increasing surfactant concentration and increased with increasing initiator concentration. Relatively high contact angles of water on films cast from the latexes showed that TREM LF‐40 did not migrate significantly to the surface of the film, which was consistent with the latex‐surface characterization results. © 2001 John Wiley & Sons, Inc. J Polym Sci Part A: Polym Chem 39: 3093–3105, 2001
Simple Microfluidic Devices for <em>in vivo</em> Imaging of <em>C. elegans</em>, <em>Drosophila</em> and Zebrafish (2012)

Micro fabricated fluidic devices provide an accessible micro-environment for in vivo studies on small organisms. Simple fabrication processes are available for microfluidic devices using soft lithography techniques. Microfluidic devices have been used for sub-cellular imaging, in vivo laser microsurgery and cellular imaging. In vivo imaging requires immobilization of organisms. This has been achieved using suction, tapered channels, deformable membranes, suction with additional cooling anesthetic gas, temperature sensitive gels, cyanoacrylate glue and anesthetics such as levamisole. Commonly used anesthetics influence synaptic transmission and are known to have detrimental effects on sub-cellular neuronal transport. In this study we demonstrate a membrane based poly-dimethyl-siloxane (PDMS) device that allows anesthetic free immobilization of intact genetic model organisms such as Caenorhabditis elegans (C. elegans), Drosophila larvae and zebrafish larvae. These model organisms are suitable for in vivo studies in microfluidic devices because of their small diameters and optically transparent or translucent bodies. Body diameters range from -10 μm to -800 μm for early larval stages of C. elegans and zebrafish larvae and require microfluidic devices of different sizes to achieve complete immobilization for high resolution time-lapse imaging. These organisms are immobilized using pressure applied by compressed nitrogen gas through a liquid column and imaged using an inverted microscope. Animals released from the trap return to normal locomotion within 10 min. We demonstrate four applications of time-lapse imaging in C. elegans namely, imaging mitochondrial transport in neurons, pre-synaptic vesicle transport in a transport-defective mutant, glutamate receptor transport and Q neuroblast cell division. Data obtained from such movies show that microfluidic immobilization is a useful and accurate means of acquiring in vivo data of cellular and sub-cellular events when compared to anesthetized animals (Figure 1J and 3C-F). Device dimensions were altered to allow time-lapse imaging of different stages of C. elegans, first instar Drosophila larvae and zebrafish larvae. Transport of vesicles marked with synaptotagmin tagged with GFP (syt.eGFP) in sensory neurons shows directed motion of synaptic vesicle markers expressed in cholinergic sensory neurons in intact first instar Drosophila larvae. A similar device has been used to carry out time-lapse imaging of heartbeat in -30 hr post fertilization (hpf) zebrafish larvae. These data show that the simple devices we have developed can be applied to a variety of model systems to study several cell biological and developmental phenomena in vivo.
Chemistry of Squaraine-Derived Materials:  Near-IR Dyes, Low Band Gap Systems, and Cation Sensors (2005)

Squaraines belong to an important class of organic dyes with intense absorption and emission properties in the visible to near-IR wavelength range. The optical properties of squaraines, which are sensitive to the surrounding medium, make them ideal candidates to photophysists to study the excited-state properties and to material chemists for designing a variety of materials that are useful for wide-ranging applications. The present Account pertains to the recent developments in the materials chemistry of squaraines, highlighting our contributions to the study of squaraine-based near-IR dyes, low band gap polymers, and cation sensors.
An active rectifier/regulator combo circuit for powering biomedical implants (2013)

A circuit that combines an active rectifier and a linear regulator, which will be referred to as a rectulator, is proposed in this paper. The main transistor in the rectulator is used for both the rectification of the AC input (V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">AC</sub> ) and the regulation of the DC output (V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">O</sub> ) to reduce the overall voltage drop between V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">AC</sub> and V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">O</sub> . The rectulator has a high power efficiency (η <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">P</sub> ) and very good amplitude modulation (AM) rejection on V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">AC</sub> against AC field strength variations. A full-wave rectulator implemented in a 0.18μm CMOS process showed an AM rejection > 45dB for an AM frequency <; 2kHz. A η <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">P</sub> of 90.7% was measured for an input frequency of 5MHz, V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">O</sub> = 2.5V and an output power of 10.5mW.
Continuously tunable laser diodes: longitudinal versus transverse tuning scheme (1990)

The operating principles and performances of the two most powerful approaches to monolithic tunable laser diodes-the longitudinally integrated three-section (3S) distributed Bragg reflector (DBR) laser and the transversely integrated tunable twin-guide (TTG) distributed feedback (DFB) laser-are described and compared. Both laser configurations are capable of being continuously tuned over several nanometers. The practically useful tuning range strongly depends on structure geometry parameters and is limited by either the required amplification or the tuning densities. It is shown that, with comparable parameters, the useful continuous tuning range of the TTG-DFB laser is 30% larger than that of the 3S-DBR laser. Wide-range continuous tuning of the TTG-DFB laser requires only a single control current, which is particularly advantageous for the manufacture and design of the wavelength control circuitry.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Low RF loss and noise of transmission lines on Si substrates using an improved ion implantation process (2003)

Very low power loss /spl les/0.6 dB at 110 GHz and noise of <0.25 dB at 18 GHz have been measured on transmission lines fabricated on Si substrates and implanted with protons. In contrast, a much worse power loss of 5 dB and higher noise of 2.5 dB were measured without implantation. This large improvement arises from the high resistivity by proton implantation, which was also done after forming the transmission lines and at a reduced energy of /spl sim/ 4 MeV for easier process integration into current VLSI technology.