gebhart/patrepeval huggingface dataset:
Purification of histidase from Streptomyces griseus and nucleotide sequence of the hutH structural gene (1992)

Histidine ammonia-lyase (histidase) was purified to homogeneity from vegetative mycelia of Streptomyces griseus. The enzyme was specific for L-histidine and showed no activity against the substrate analog, D-histidine. Histidinol phosphate was a potent competitive inhibitor. Histidase displayed saturation kinetics with no detectable sigmoidal response. Neither thiol reagents nor a variety of divalent cations had any effect on the activity of the purified enzyme. High concentrations of potassium cyanide inactivated histidase in the absence of its substrate or histidinol phosphate, suggesting that, as in other histidases, dehydroalanine plays an important role in catalysis. The N-terminal amino acid sequence of histidase was used to construct a mixed oligonucleotide probe to identify and clone the histidase structural gene, hutH, from genomic DNA of the wild-type strain of S. griseus. The cloned DNA restored the ability of a histidase structural gene mutant to grow on L-histidine as the sole nitrogen source. The deduced amino acid sequence of hutH shows significant relatedness with histidase from bacteria and a mammal as well as phenylalanine ammonia-lyase from plants and fungi.
Ex vivo analysis identifies effective HIV-1 latency–reversing drug combinations (2015)

Reversal of HIV-1 latency by small molecules is a potential cure strategy. This approach will likely require effective drug combinations to achieve high levels of latency reversal. Using resting CD4+ T cells (rCD4s) from infected individuals, we developed an experimental and theoretical framework to identify effective latency-reversing agent (LRA) combinations. Utilizing ex vivo assays for intracellular HIV-1 mRNA and virion production, we compared 2-drug combinations of leading candidate LRAs and identified multiple combinations that effectively reverse latency. We showed that protein kinase C agonists in combination with bromodomain inhibitor JQ1 or histone deacetylase inhibitors robustly induce HIV-1 transcription and virus production when directly compared with maximum reactivation by T cell activation. Using the Bliss independence model to quantitate combined drug effects, we demonstrated that these combinations synergize to induce HIV-1 transcription. This robust latency reversal occurred without release of proinflammatory cytokines by rCD4s. To extend the clinical utility of our findings, we applied a mathematical model that estimates in vivo changes in plasma HIV-1 RNA from ex vivo measurements of virus production. Our study reconciles diverse findings from previous studies, establishes a quantitative experimental approach to evaluate combinatorial LRA efficacy, and presents a model to predict in vivo responses to LRAs.
Quantitative Phase Analysis of Synthetic Silicon Nitride by X-Ray Diffraction (1979)

Synthetically prepared silicon nitride is one of the more promising ceramic materials for structural components of gas turbines. Typical material may contain a-silicon nitride, Si 3 N 4 (which is believed to always contain oxygen and therefore, according to Grievson, Jack and Wild, is more properly written as Si 11.5 N 15 O 0.5 ), β-silicon nitride, Si 3 N 4 , silicon oxynitride, Si 2 ON 2 , silicon metal, Si, and α-cristobalite, SiO 2 . Because the physical properties of the ceramic parts are dependent on their phase composition, it is essential that a technique be available for performing a phase analysis. An X-ray diffraction procedure has been, developed for the quantitative phase analysis of synthetically prepared silicon nitride. This procedure converts experimentally measured intensities of selected X-ray diffraction peaks to weight fractions of components using empirically determined intensity coefficients.
The Association of Weight Change in Alzheimer's Disease with Severity of Disease and Mortality: A Longitudinal Analysis (1998)

The risk of weight loss tends to increase with severity and progression of AD. Weight loss is a predictor of mortality among subjects with AD, whereas weight gain appears to have a protective effect.
<i>Deinococcales</i> (2015)

Abstract Dei.no.coc.ca'les. M.L. masc. n. Deinococcus type genus of the order; ‐ ales ending to denote an order; M.L. fem. pl. n. Deinococcales the Deinococcus order. Taxonomic and Nomenclature Notes According to the List of Prokaryotic names with Standing in Nomenclature (LPSN), the taxonomic status of the order Deinococcales is: correct name (last update, February 2025) * . LPSN classification: Bacteria / Thermotogati / Deinococcota / Deinococci / Deinococcales The order Deinococcales can also be recovered in the Genome Taxonomy Database (GTDB) as o__Deinococcales (version v220) ** . GTDB classification: d__Bacteria / p__Deinococcota / c__Deinococci / o__Deinococcales * Meier‐Kolthoff et al. ( 2022 ). Nucleic Acids Res , 50 , D801 – D807 ; DOI: 10.1093/nar/gkab902 ** Parks et al. ( 2022 ). Nucleic Acids Res , 50 , D785 – D794 ; DOI: 10.1093/nar/gkab776
Design and control of a robotic exoskeleton form gait rehabilitation (2013)

Tesis de Máster escrita para la obtención del Título de Grado en el Official Master in Robotics and Automation. Universidad Carlos III de Madrid. xxiii, 71 p. : il., diagr.
A Hierarchical Bayesian Model for Learning Nonlinear Statistical Regularities in Nonstationary Natural Signals (2005)

Capturing statistical regularities in complex, high-dimensional data is an important problem in machine learning and signal processing. Models such as principal component analysis (PCA) and independent component analysis (ICA) make few assumptions about the structure in the data and have good scaling properties, but they are limited to representing linear statistical regularities and assume that the distribution of the data is stationary. For many natural, complex signals, the latent variables often exhibit residual dependencies as well as nonstationary statistics. Here we present a hierarchical Bayesian model that is able to capture higher-order nonlinear structure and represent nonstationary data distributions. The model is a generalization of ICA in which the basis function coefficients are no longer assumed to be independent; instead, the dependencies in their magnitudes are captured by a set of density components. Each density component describes a common pattern of deviation from the marginal density of the pattern ensemble; in different combinations, they can describe nonstationary distributions. Adapting the model to image or audio data yields a nonlinear, distributed code for higher-order statistical regularities that reflect more abstract, invariant properties of the signal.
Basis material decomposition in spectral CT using a semi-empirical, polychromatic adaption of the Beer–Lambert model (2016)

Following the development of energy-sensitive photon-counting detectors using high-Z sensor materials, application of spectral x-ray imaging methods to clinical practice comes into reach. However, these detectors require extensive calibration efforts in order to perform spectral imaging tasks like basis material decomposition. In this paper, we report a novel approach to basis material decomposition that utilizes a semi-empirical estimator for the number of photons registered in distinct energy bins in the presence of beam-hardening effects which can be termed as a polychromatic Beer-Lambert model. A maximum-likelihood estimator is applied to the model in order to obtain estimates of the underlying sample composition. Using a Monte-Carlo simulation of a typical clinical CT acquisition, the performance of the proposed estimator was evaluated. The estimator is shown to be unbiased and efficient according to the Cramér-Rao lower bound. In particular, the estimator is capable of operating with a minimum number of calibration measurements. Good results were obtained after calibration using less than 10 samples of known composition in a two-material attenuation basis. This opens up the possibility for fast re-calibration in the clinical routine which is considered an advantage of the proposed method over other implementations reported in the literature.
Unipoled Disk-type Piezoelectric Transformers (2002)

Unipoled piezoelectric transformers with different input and output area ratios were fabricated. The electrical properties in terms of voltage step-up ratio, output power, efficiency, and temperature rise were measured at various load resistance. The voltage step-up ratio increased proportionally with load resistance. The relative efficiency showed a maximum value of more than 98% in the transformer, which is 23.6 mm in diameter and 1.0 mm in thickness with input/out electrode area ratio of 0.62. With the driving voltage of 35 Vrms, the 8.1 W output power was obtained in the transformer with an input/output electrodes area ratio of 6.47. In such conditions, the temperature rise was only 15°C from room temperature. This transformer, which has approximately ten times higher power density than a rectangular Rosen-type piezoelectric transformer, was successfully applied to operate an 8 W compact fluorescent lamp.
Effect of sand and wood-shavings bedding on the behavior of broiler chickens (2005)

The purpose of this study was to determine the effect of 2 different bedding types, sand and wood shavings, on the behavior of broiler chickens. In experiment 1, 6 pens were divided down the center and bedded half with sand and half with wood shavings. Male broilers (10/pen) were observed by scan sampling at 5- or 12-min intervals throughout the 6-wk growth period during the morning (between 0800 to 0900 h), afternoon (1200 to 1500 h), and night (2300 to 0600 h). There was a significant behavior x substrate x week interaction during the day (P < 0.0001) and at night (P < 0.0002). Drinking, dustbathing, preening, and sitting increased in frequency on the sand side but decreased on the wood shavings side during the day, as did resting at night. In general, broilers performed a greater proportion of their total behavioral time budget on the sand (P < 0.0001) as they aged. Broilers used the divider between the 2 bedding types to perch; perching behavior peaked during wk 4. In experiment 2, male broilers were housed in 8 pens (50 birds/pen) bedded only in sand or wood shavings. Bedding type had no effect on behavioral time budgets (P = 0.8946), although there were age-related changes in behavior on both bedding types. These results indicate that when given a choice, broilers increasingly performed many of their behaviors on sand, but if only one bedding type was provided they performed those behaviors with similar frequency on sand or wood shavings.
The sonographic evaluation of tubal patency with stimulated acoustic emission imaging (2002)

SAE techniques were successfully applied to HyCoSy and allowed the visualization of the free spill of contrast agent into the peritoneal cavity in the majority of cases. SAE-HyCoSy showed good agreement with HSG in this preliminary study.
Bioelectricity and Regeneration (1979)

A wide variety of developing systems drive large steady electric current through themselves. In particular, the stumps of amputated amphibian limbs drive large currents out their cut ends. The effects of modulating these stump currents suggest that they help initiate regeneration.
Synergistic therapeutic effects of combined adenovirus‐mediated interleukin‐10 and interleukin‐12 gene therapy on airway inflammation in asthmatic mice (2009)

Taken together, the results obtained in the present study indicate that co-administration of IL-12 and IL-10 may have therapeutic potential for the immunomodulatory treatment of allergic asthma.
Separation of Alcohol-Soluble Proteins (Zeins) from Maize into Three Fractions by Differential Solubility (1986)

The prolamin of maize (Zea mays L.), zein, was extracted from endosperm meal with 60% (v/v) 2-propanol/1% (v/v) 2-mercaptoethanol either directly or subsequent to extraction with 90% (v/v) 2-propanol. The zein extracted with 90% 2-propanol was essentially made up of 20 to 24 kilodalton polypeptides (alpha-zein) while that extractable with 60% 2-propanol/1% 2-mercaptoethanol contained, in addition to alpha-zein, 17 to 18 kilodalton methionine-rich polypeptides and a 27 kilodalton proline-rich polypeptide. While zein was separated into three fractions by differential solubility in 90% 2-propanol and 30% 2-propanol/30 millimolar sodium acetate (pH 6) using two different fractionation protocols. Each of the three solubility fractions (SF1, SF2, and SF3) had a unique polypeptide composition. Based on results obtained from two inbreds, K55 and W64A, the SF1 constituted 75 to 80% of the total zein and included as major components 20 to 24 kilodalton polypeptides and a minor 10 kilodalton polypeptide. The SF2 made up 10 to 15% of the total zein and included exclusively 17 to 18 kD methionine-rich polypeptides. A 27 kilodalton proline-rich component constituted the SF3 and contributed 5 to 10% to total zein.
A Research and Construction on the Grid Resources Managing Model Based on Multi-Agent (2005)

With the maturity of Intemet technique, the presumption of grid computing has aroused attention throughout the world. However, the key factor of grid computing is to mediate among non-centralized controlled resources, to guarantee the high quality of service (QoS)through the standardized, open and generalized protocol and interface. This paper is trying to explore the Grid Resources Managing Model of distributed node autonomy based on Multi-Agent Technique, to provide the user with a distributive, easily extended, highly efficient, and practical Internet computing environment through the application of this model to the internet environment on campus.
Length‐dependent DNA separations using multiple end‐attached peptide nucleic acid amphiphiles in micellar electrokinetic chromatography (2008)

End-labeled free-solution electrophoresis (ELFSE) is an alternative approach to gel-based methods for size-based electrophoretic separation of DNA. In ELFSE, an electrically neutral "drag-tag" is appended to DNA to add significant hydrodynamic drag, thereby breaking its constant charge-to-friction ratio. Current drag-tag architecture relies on covalent attachment of polymers to each DNA molecule. We have recently proposed the use of micellar drag-tags in conjunction with sequence-specific hybridization of peptide nucleic acid amphiphiles (PNAAs). This work investigates the effect of multiple PNAA attachment on DNA resolution using MEKC. Simultaneous PNAA hybridization allows for the separation of long DNA targets, up to 1012 bases, using micellar drag-tags. Each PNAA handle independently interacts with the micellar phase, reducing the overall mobility of this complex relative to individual PNAA binding. The sequence- and size-based dependence of this separation technique is maintained with multiple PNAA binding over a range of DNA sizes. Results are accurately described by ELFSE theory, yielding alpha=54 for single-micelle tagging and alpha=142 for dual-micelle tagging. This method is the first example of a non-covalent drag-tag used to separate DNA of 1000 bases based on both size and sequence.
Museum recommendation system based on lifestyles (2008)

The existing recommendation system has been widely applied on electronic business field. In recent years, such system has been gradually stepped into the digital teaching field, the advantage of which is to know the preference and interest etc., factors of the learner so as to provide the learner with suitable learning contents. Due to the increasing number of new users and new displaying content, the recommendation system tends to confront with a situation that is incapable and invalid recommendation called ¿cold start¿ problem. In order to solve such problem, we introduce from the viewpoint of sociology to incorporate the lifestyle of the users into the museum recommendation system. This will enable the users inside the museum with a personalized learning service. The character of this system is to take the lifestyle of the users into consideration instead of the system act, further, to analyze the characteristics and to study the favorite displaying content of different clusters through questionnaire survey to take the lifestyle and cluster preference contents as the principle of recommendation items.
The many faces of Epstein-Barr virus (1988)

Epstein-Barr virus (EBV) persists for life in a person who has been infected. Investigators are uncertain what this means to the host, particularly an immunocompromised one. EBV is the most common cause of infectious mononucleosis, but the diagnosis must be based on clinical, hematologic, and serologic criteria because other agents are the cause in about 10% of cases. EBV is the first virus to be associated with a neoplasm--Burkitt's lymphoma. This childhood malignancy is relatively common in central Africa, and EBV is a cofactor in its development, although direct evidence for a causal relationship has not been found. In southern China, nasopharyngeal carcinoma is an important health problem in adults in areas where the infection rate of EBV in childhood is high. Rare cases of primary EBV infection that evolved into uncontrolled lymphoproliferative disease have also been reported. EBV's relationship to these diverse diseases, its varying effects on certain individuals and in certain geographic locations, and the testing of a vaccine against the virus are areas of ongoing study.
Designing Babies: Human Research Issues (2004)

A PUBLICATION OF THE HASTINGS CENTER uman genetic engineering was one of the earliest bioethics topics to attract widespread attention. During the 1960's, scientists and scholars began examining the possible benefits and harms that could come from attempts at human genetic alteration.' Germline genetic alterations attracted the most controversy. Genetic alterations made in earlystage human embryos would be present in the cells of children developing from such embryos, including the germ (sperm or egg) cells. As a result, this form of genetic alteration could affect not only children developing from genetically modified embryos, but their descendants, too. The long-running debate over the ethics of germline genetic alteration has focused on the technology's broad social implications, such as potential effects on parent-child relations, social inequalities, and the human gene pool.z These broad implications are only part of the story, however. Extensive human subjects research would be required to bring germline genetic alterations to the clinic. Yet the ethics of conducting such research has received comparatively little attention.
Thymoquinone as an anticancer agent: evidence from inhibition of cancer cells viability and invasion in vitro and tumor growth <i>in vivo</i> (2012)

Phytochemical compounds are emerging as a new generation of anticancer agents with limited toxicity in cancer patients. The purpose of this study was to investigate the potential impact of thymoquinone (TQ), the major constituent of black seed, on survival, invasion of cancer cells in vitro, and tumor growth in vivo. Exposure of cells derived from lung (LNM35), liver (HepG2), colon (HT29), melanoma (MDA-MB-435), and breast (MDA-MB-231 and MCF-7) tumors to increasing TQ concentrations resulted in a significant inhibition of viability through the inhibition of Akt phosphorylation leading to DNA damage and activation of the mitochondrial-signaling proapoptotic pathway. We provide evidence that TQ at non-toxic concentrations inhibited the invasive potential of LNM35, MDA-MB-231, and MDA-MB231-1833 cancer cells. Moreover, we demonstrate that TQ synergizes with DNA-damaging agent cisplatin to inhibit cellular viability. The anticancer activity of thymoquinone was also investigated in athymic mice inoculated with the LNM35 lung cells. Administration of TQ (10 mg/kg/i.p.) for 18 days inhibited the LNM35 tumor growth by 39% (P < 0.05). Tumor growth inhibition was associated with significant increase in the activated caspase-3. The in silico target identification suggests several potential targets of TQ mainly HDAC2 proteins and the 15-hydroxyprostaglandin dehydrogenase. In this context, we demonstrated that TQ treatment resulted in a significant inhibition of HDAC2 proteins. In view of the available experimental findings, we contend that thymoquinone and/or its analogues may have clinical potential as an anticancer agent alone or in combination with chemotherapeutic drugs such as cisplatin.
Monitoring the performance of commercial T1-rate transmission service (1991)

This paper gathers the scattered empirical and theoretical elements of the performance-management problem for commercial T1-rate transmission service and integrates these elements in a useful way. We propose two variants of a time-based performance-monitoring algorithm that are insensitive to the arrival pattern of transmission errors. The first variant compares a count of errored seconds accumulated over an interval of time to a fixed threshold, and issues an alert to the network operator indicating degraded transmission performance whenever the count exceeds the threshold before the measurement interval expires. The fixed-threshold test is calibrated with reference to the well-known Neyman model of transmission errors on metallic-conductor systems. This calibration is then shown to be suitable as well for monitoring the performance of fiber-optic transmission systems where errored seconds follow the cumulative binomial distribution. The second variant of the new performance-monitoring algorithm replaces the fixed-threshold test with a dual-threshold test having a lower threshold that remains fixed and a higher threshold that floats in response to changes in error characteristics. An analysis based on the difference equations that describe the movement of the floating threshold shows that the dual-threshold test is more responsive than the fixed-threshold test in detecting nonstationary trends toward degraded transmission and in detecting stable but mediocre performance levels.
Long-chain acyl-CoA esters and acyl-CoA binding protein are present in the nucleus of rat liver cells (2000)

A detailed analysis of the subcellular distribution of acyl-CoA esters in rat liver revealed that significant amounts of long-chain acyl-CoA esters are present in highly purified nuclei. No contamination of microsomal or mitochondrial marker enzymes was detectable in the nuclear fraction. C16:1 and C18:3-CoA esters were the most abundant species, and thus, the composition of acyl-CoA esters in the nuclear fraction deviates notably from the overall composition of acyl-CoA esters in the cell. After intravenous administration of the non-beta-oxidizable [(14)C]tetradecylthioacetic acid (TTA), the TTA-CoA ester could be recovered from the nuclear fraction. Acyl-CoA esters bind with high affinity to the ubiquitously expressed acyl-CoA binding protein (ACBP), and several lines of evidence suggest that ACBP functions as a pool former and transporter of acyl-CoA esters in the cytoplasm. By using immunohistochemistry, immunofluorescence microscopy, and immunoelectron microscopy we demonstrate that ACBP localizes to the nucleus as well as the cytoplasm of rat liver cell and rat hepatoma cells, suggesting that ACBP may also be involved in regulation of acyl-CoA-dependent processes in the nucleus.
Gold Superfill in Sub-Micrometer Trenches (2005)

Bottom-up feature fill, also called "superfill," of gold is demonstrated for the first time. Gold superfill is achieved in trenches as narrow as 60 nm, particularly relevant for interconnects on GaAs and GaN technologies. This was accomplished through the use of submonolayer coverage of a preadsorbed, deposition-rate accelerating surfactant followed by Au electrodeposition. The experimental results indicate the potential of this technologically relevant process and also support the generality of the CEAC mechanism for feature superfill and surface stabilization. © 2005 The Electrochemical Society. All rights reserved.