gebhart/patrepeval huggingface dataset:
P element transposition in Drosophila melanogaster: an analysis of sister-chromatid pairs and the formation of intragenic secondary insertions during meiosis. (1993)

The gap-repair model proposes that P elements move via a conservative, "cut-and-paste" mechanism followed by double-strand gap repair, using either the sister chromatid or homolog as the repair template. We have tested this model by examining meiotic perturbations of an X-linked ry+ transposon during the meiotic cycle of males, employing the mei-S332 mutation, which induces high frequency equational nondisjunction. This system permits the capture of both sister-X chromatids in a single patroclinous daughter. In the presence of P-transposase, transpositions within the immediate proximity of the original site are quite frequent. These are readily detectable among the patroclinous daughters, thereby allowing the combined analysis of the transposed element, the donor site and the putative sister-strand template. Molecular analysis of 22 meiotic transposition events provide results that support the gap-repair model of P element transposition. Prior to this investigation, it was not known whether transposition events were exclusively or predominantly premeiotic. The results of our genetic analysis revealed that P elements mobilize at relatively high frequencies during meiosis. We estimated that approximately 4% of the dysgenic male gametes have transposon perturbations of meiotic origin; the proportion of gametes containing lesions of premeiotic origin was estimated at 32%.
Seronegative patients vaccinated with cytomegalovirus gB-MF59 vaccine have evidence of neutralising antibody responses against gB early post-transplantation (2019)

We hypothesise that the vaccine primed the immune system of seronegative recipients which, when further challenged with virus at time of transplant, allowed the host to mount rapid immunological humoral responses even under conditions of T cell immune suppression during transplantation.
Symbolic partitioning and scheduling of parameterized task graphs (2002)

The DAG based task graph model has been found effective in scheduling for performance prediction and optimization of parallel applications. However the scheduling complexity and solution normally depend on the problem size. We propose a symbolic scheduling scheme for a parameterized task graph which models coarse grain DAG parallelism, independent of the problem size. The algorithm first derives symbolic clusters to a group of tasks in order to minimize communication while preserving parallelism, and then it evenly assigns task clusters to processors. The run time system executes clusters on each processor in a multithreaded fashion. The paper also presents preliminary experimental results to demonstrate the effectiveness of our techniques.
Spherical Four-Bar Linkage Mechanism for Continuous Passive Movement Rehabilitation Treatment of the Ankle (1996)

Abstract Continuous passive movement (CPM) is an important process in the post-operative rehabilitation treatment of the knee. The two-dimensional, flexion/extension motion provided by CPM has been shown to have beneficial effects on tendon, cartilage, and ligament health via increased blood flow to the knee and surrounding tissues. Similarly, CPM may be useful in the rehabilitation of the ankle. However, the ball and socket joint of the ankle would benefit more from a three-dimensional spherical motion instead of the traditional two-dimensional, flexion/extension motion. This study presents a four-bar linkage developed with the use of three dimensional mechanical modeling software. The linkage provides a spherical path of motion for the continuous passive movement of the ankle. This spherical CPM may ultimately be useful in the treatment of congenital musculoskeletal abnormalities, acquired joint disorders, and traumatic injury of the ankle.
Wafer fused p-InP/p-GaAs heterojunctions (1998)

This article reports on the fabrication and characterization of wafer fused heterojunctions between p-InP and p-GaAs. Secondary ion mass spectroscopy was used to characterize doping profiles across the interface as well as the interface contamination with oxygen or carbon. The crystalline quality of the fused material was characterized using cross section and plan-view transmission electron microscopy. The electrical properties of the fused interface were studied as a function of various doping elements such as Be and Zn in InP or Zn and C in GaAs as well as for different acceptor concentrations in GaAs. Finally, the electrical characteristics were analyzed using a numerical model that includes thermionic emission and tunneling across the heterobarrier.
Improved temperature dependence of 1.3 µmAlGaInAs-based MQW semiconductor diode lasers revealed by hydrostatic pressure (1998)

The threshold current Ith of 1.3 µm AlGaInAs devices increases by ~8% over a 1 GPa pressure range contrasting with decreases of 10–15% in 1.3 µm InGaAsP devices. This can be explained with 50% nonradiative recombination in InGaAsP and only ~20% in AlGaInAs devices, resulting in a much improved temperature dependence of Ith.
Ontology-Based Information Behaviour to Improve Web Search (2010)

Web Search Engines provide a huge number of answers in response to a user query, many of which are not relevant, whereas some of the most relevant ones may not be found. In the literature several approaches have been proposed in order to help a user to find the information relevant to his/her real needs on the Web. To achieve this goal the individual Information Behavior can been analyzed to ’keep’ track of the user’s interests. Keeping information is a type of Information Behavior, and in several works researchers have referred to it as the study on what people do during a search on the Web. Generally, the user’s actions (e.g., how the user moves from one Web page to another, or her/his download of a document, etc.) are recorded in Web logs. This paper reports on research activities which aim to exploit the information extracted from Web logs (or query logs) in personalized user ontologies, with the objective to support the user in the process of discovering Web information relevant to her/his information needs. Personalized ontologies are used to improve the quality of Web search by applying two main techniques: query reformulation and re-ranking of query evaluation results. In this paper we analyze various methodologies presented in the literature aimed at using personalized ontologies, defined on the basis of the observation of Information Behaviour to help the user in finding relevant information.
Interleukin 10 suppresses experimental chronic, granulomatous inflammation induced by bacterial cell wall polymers. (1996)

These results indicate that exogenous IL10 can inhibit experimental granulomatous inflammatory responses and suggest that IL10 treatment could be an effective new therapeutic approach in human disorders such as Crohn's disease, rheumatoid arthritis, and sarcoidosis.
Gingival crevicular fluid EMAP‐II, MIP‐1<i>α</i> and MIP‐1<i>β</i> levels of patients with periodontal disease (2005)

Our results suggest that elevated GCF EMAP-II could contribute to the pathogenesis of G-AgP. Alternatively, EMAP-II reflects the extent of the inflammatory activity in the periodontal tissues. At this point, MIP-1alpha and MIP-1beta levels in GCF do not seem to play a discriminatory role in periodontitis. Our data document for the first time the essential role of EMAP-II in the pathogenesis of different periodontal diseases.
Behavior-Decision Fuzzy Algorithm with the Ambiguous State Adaptability for Autonomous Mobile Robots (1993)

知能ロボットにおいて「人間らしい」行動決定機能を表現しようとする場合、あいまい状況認識を含む動作シーケンスの記述が重要となる。本研究では、クリスプ量およびファジィ量が混在するようなシーケンスフローが表現可能なファジィアルゴリズムの概念を用いて、人間が通常行っているのに近いマクロ行動決定アルゴリズムを記述することを試みる。本論文では、まずアルゴリズムチューニング機能を有する修正ファジィアルゴリズムを提案し、次にこれを用いた自律移動ロボットの行動決定アルゴリズムの構築方法について述べる。本方式の主な特長を以下に示す。1)ファジィアルゴリズムを用いることにより行動決定シーケンスの柔軟でかつマクロな知識表現が可能である。2)ファジィフレームによるあいまい概念および修正ファジィアルゴリズムによるあいまい状況の定義ができる。3)行動目的に従って制御されるスレショールド値をもつアルゴリズムチューニング機能をもつ。最後に本方式の検証のため、簡単な屋内環境を想定して行った計算機シミュレーションおよび移動実験の結果についても報告する。
Determination of Bacteriophage Genome Size by Pulsed-Field Gel Electrophoresis (2009)

Standard agarose gel electrophoresis is extensively used to resolve DNA fragments from 0.2 to 40-50 kb. Larger fragments of genomic DNA or whole viral genomes can only effectively be resolved by pulsed-field gel electrophoresis (PFGE), which extends the range of molecular separation from 200 bp to 12 Mb.
Translocation of globin fusion proteins across the endoplasmic reticulum membrane in Xenopus laevis oocytes. (1987)

We have studied the translocation of a normally cytoplasmic protein domain across the membrane of the endoplasmic reticulum in cell-free systems and in Xenopus laevis oocytes. Coding regions for the normally cytoplasmic protein globin were engineered in frame either 3' or 5' to the coding region for the signal sequence of either Escherichia coli b-lactamase or bovine preprolactin, respectively, in SP6 expression plasmids. RNA transcribed from these plasmids was microinjected into oocytes as well as translated in cell-free systems. We demonstrate that both in vivo and in vitro, a previously amino-terminal signal sequence can direct translocation of domains engineered to either side. Moreover, the domain preceding the signal sequence can be as large as that which follows it. While, in general, cell-free systems were found to faithfully reflect translocation events in vivo, our results suggest that a mechanism for clearance of signal peptides after cleavage is present in intact cells that is not reconstituted in cell-free systems.
Evaluating alternative hypotheses for the early evolution and diversification of ants (2006)

Ants are the world's most diverse and ecologically dominant eusocial organisms. Resolving the phylogeny and timescale for major ant lineages is vital to understanding how they achieved this success. Morphological, molecular, and paleontological studies, however, have presented conflicting views on early ant evolution. To address these issues, we generated the largest ant molecular phylogenetic data set published to date, containing approximately 6 kb of DNA sequence from 162 species representing all 20 ant subfamilies and 10 aculeate outgroup families. When these data were analyzed with and without outgroups, which are all distantly related to ants and hence long-branched, we obtained conflicting ingroup topologies for some early ant lineages. This result casts strong doubt on the existence of a poneroid clade as currently defined. We compare alternate attachments of the outgroups to the ingroup tree by using likelihood tests, and find that several alternative rootings cannot be rejected by the data. These alternatives imply fundamentally different scenarios for the early evolution of ant morphology and behavior. Our data strongly support several notable relationships within the more derived formicoid ants, including placement of the enigmatic subfamily Aenictogitoninae as sister to Dorylus army ants. We use the molecular data to estimate divergence times, employing a strategy distinct from previous work by incorporating the extensive fossil record of other aculeate Hymenoptera as well as that of ants. Our age estimates for the most recent common ancestor of extant ants range from approximately 115 to 135 million years ago, indicating that a Jurassic origin is highly unlikely.
Role and Regulation of PDGFRα Signaling in Liver Development and Regeneration (2013)

Aberrant platelet-derived growth factor receptor-α (PDGFRα) signaling is evident in a subset of hepatocellular cancers (HCCs). However, its role and regulation in hepatic physiology remains elusive. In the current study, we examined PDGFRα signaling in liver development and regeneration. We identified notable PDGFRα activation in hepatic morphogenesis that, when interrupted by PDGFRα-blocking antibody, led to decreased hepatoblast proliferation and survival in embryonic liver cultures. We also identified temporal PDGFRα overexpression, which is regulated by epidermal growth factor (EGF) and tumor necrosis factor α, and its activation at 3 to 24 hours after partial hepatectomy. Through generation of hepatocyte-specific PDGFRA knockout (KO) mice that lack an overt phenotype, we show that absent PDGFRα compromises extracelluar signal-regulated kinases and AKT activation 3 hours after partial hepatectomy, which, however, is alleviated by temporal compensatory increases in the EGF receptor (EGFR) and the hepatocyte growth factor receptor (Met) expression and activation along with rebound activation of extracellular signal-regulated kinases and AKT at 24 hours. These untimely increases in EGFR and Met allow for normal hepatocyte proliferation at 48 hours in KO, which, however, are aberrantly prolonged up to 72 hours. Intriguingly, such compensation also was visible in primary KO hepatocyte cultures but not in HCC cells after siRNA-mediated PDGFRα knockdown. Thus, temporal activation of PDGFRα in liver development is important in hepatic morphogenesis. In liver regeneration, despite increased signaling, PDGFRα is dispensable owing to EGFR and Met compensation, which is unique to normal hepatocytes but not HCC cells.
The <i>In Vitro</i> and <i>In Vivo</i> Ocular Pharmacology of Olopatadine (AL-4943A), an Effective Anti-Allergic/Antihistaminic Agent (1996)

Olopatadine (AL-4943A; KW-4679) [(z)-11-[3-(dimethylamino)propylidene]-6, 11-dihydrodibenz[b,e]oxepine-2 acetic acid hydrochloride] is an anti-allergic agent which inhibits mast cell mediator release and possesses histamine H1 receptor antagonist activity. Studies were conducted to characterize the in vitro and in vivo pharmacological profile of this drug relevant to its topical ocular use. AL-4943A inhibits histamine release in a concentration-dependent fashion (IC50 = 559 microM) from human conjunctival mast cell preparations in vitro. Histamine release was not stimulated by AL-4943A at concentrations as high as 10 mM. In contrast, ketotifen stimulated histamine release at concentrations slightly higher than effective inhibitory concentrations. AL-4943A did not display any in vitro cyclooxygenase or 5-lipoxygenase inhibition. Topical ocular application of AL-4943A effectively inhibits antigen- and histamine-stimulated conjunctivitis in guinea pigs. Passive anaphylaxis in guinea pig conjunctiva was attenuated by AL-4943A applied 30 min prior to intravenous or topical ocular antigen challenge (ED50 values 0.0067% and 0.0170%, w/v, respectively). Antihistaminic activity in vivo was demonstrated using a model of histamine-induced vascular permeability in guinea pig conjunctiva. AL-4943A applied topically from 5 min to 24 hrs prior to histamine challenge effectively and concentration-dependently inhibited the vascular permeability response, indicating the compound has an acceptable onset and a long duration of action. Drug concentrations 5-fold greater than those effective against histamine-stimulated conjunctival responses failed to inhibit vascular permeability responses induced with either serotonin or Platelet-Activating-Factor. These data indicate that the anti-histaminic effect observed with AL-4943A is specific. These anti-allergic/antihistaminic activities of AL-4943A observed in preclinical model systems have been confirmed in clinical trials in allergic patients.
Controlling Heterojunction Abruptness in VLS-Grown Semiconductor Nanowires via in situ Catalyst Alloying (2011)

For advanced device applications, increasing the compositional abruptness of axial heterostructured and modulation doped nanowires is critical for optimizing performance. For nanowires grown from metal catalysts, the transition region width is dictated by the solute solubility within the catalyst. For example, as a result of the relatively high solubility of Si and Ge in liquid Au for vapor-liquid-solid (VLS) grown nanowires, the transition region width between an axial Si-Ge heterojunction is typically on the order of the nanowire diameter. When the solute solubility in the catalyst is lowered, the heterojunction width can be made sharper. Here we show for the first time the systematic increase in interface sharpness between axial Ge-Si heterojunction nanowires grown by the VLS growth method using a Au-Ga alloy catalyst. Through in situ tailoring of the catalyst composition using trimethylgallium, the Ge-Si heterojunction width is systematically controlled by tuning the semiconductor solubility within a metal Au-Ga alloy catalyst. The present approach of alloying to control solute solubilities in the liquid catalyst may be extended to increasing the sharpness of axial dopant profiles, for example, in Si-Ge pn-heterojunction nanowires which is important for such applications as nanowire tunnel field effect transistors or in Si pn-junction nanowires.
Optically active solvents in nuclear magnetic resonance spectroscopy. IX. Direct determinations of optical purities and correlations of absolute configurations of .alpha.-amino acids (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOptically active solvents in nuclear magnetic resonance spectroscopy. IX. Direct determinations of optical purities and correlations of absolute configurations of .alpha.-amino acidsWilliam H. Pirkle and Steven D. BeareCite this: J. Am. Chem. Soc. 1969, 91, 18, 5150–5155Publication Date (Print):August 1, 1969Publication History Published online1 May 2002Published inissue 1 August 1969https://pubs.acs.org/doi/10.1021/ja01046a036https://doi.org/10.1021/ja01046a036research-articleACS PublicationsRequest reuse permissionsArticle Views469Altmetric-Citations99LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
New Class I and II HLA Alleles Strongly Associated with Opposite Patterns of Progression to AIDS (1999)

The genetics of resistance to infection by HIV-1 cohort consists of 200 slow and 75 rapid progressors to AIDS corresponding to the extremes of HIV disease outcome of 20,000 Caucasians of European descent. A comprehensive analysis of HLA class I and class II genes in this highly informative cohort has identified HLA alleles associated with fast or slow progression, including several not described previously. A quantitative analysis shows an overall HLA influence independent of and equal in magnitude (for the protective effect) to the effect of the CCR5-Delta32 mutation. Among HLA class I genes, A29 (p = 0.001) and B22 (p < 0.0001) are significantly associated with rapid progression, whereas B14 (p = 0.001) and C8 (p = 0.004) are significantly associated with nonprogression. The class I alleles B27, B57, C14 (protective), and C16, as well as B35 (susceptible), are also influential, but their effects are less robust. Influence of class II alleles was only observed for DR11. These results confirm the influence of the immune system on disease progression and may have implications on peptide-based vaccine development.
Correlative NAD(P)H‐FLIM and oxygen sensing‐PLIM for metabolic mapping (2016)

Cellular responses to oxygen tension have been studied extensively. Oxygen tension can be determined by considering the phosphorescence lifetime of a phosphorescence sensor. The simultaneous usage of FLIM of coenzymes as NAD(P)H and FAD(+) and PLIM of oxygen sensors could provide information about correlation of metabolic pathways and oxygen tension. We investigated correlative NAD(P)H-FLIM and oxygen sensing-PLIM for simultaneously analyzing cell metabolism and oxygen tension. Cell metabolism and pO2 were observed under different hypoxic conditions in squamous carcinoma cell cultures and in complex ex vivo systems. Increased hypoxia induced an increase of the phosphorescence lifetime of Ru(BPY)3 and in most cases a decrease in the lifetime of NAD(P)H which is in agreement to the expected decrease of the protein-bound NAD(P)H during hypoxia. Oxygen was modulated directly in the mitochondrial membrane. Blocking of complex III and accumulation of oxygen could be observed by both the decrease of the phosphorescence lifetime of Ru(BPY)3 and a reduction of the lifetime of NAD(P)H which was a clear indication of acute changes in the redox state of the cells. For the first time simultaneous FLIM/PLIM has been shown to be able to visualize intracellular oxygen tension together with a change from oxidative to glycolytic phenotype.
Ubiquitous grid resource monitoring (2004)

In any wide-area distributed system there is a need to communicate and interact with a range of networked devices and services ranging from computer-based ones (CPU, memory and disk), to network components (hubs, routers, gateways) and specialised data sources (embedded devices, sensors, data-feeds). In order for the ensemble of underlying technologies to provide an environment suitable for virtual organisations to flourish, the resources that comprise the fabric of the Grid must be monitored in a seamless manner that abstracts away from the underlying complexity. Furthermore, as various competing Grid middleware offerings are released and evolve, an independent overarching monitoring service should act as a corner stone that ties these systems together. GridRM is a standards-based approach that is independent of any given middleware and that can utilise legacy and emerging resource-monitoring technologies. The main objective of the project is to produce a standardised and extensible architecture that provides seamless mechanisms to interact with native monitoring agents across heterogeneous resources. 1.
Nonlinear Prediction of Head Movements for Helmet-Mounted Displays (1983)

Abstract : In head-and eye-slaved visual systems, lag times in the visual feedback loop are more apparent than they are in conventional fixed display systems. The available technology of digital image generators does not permit lag times to be reduced to the required amount. Therefore, appropriate prediction algorithms have to be developed. Accelerometers were used to measure the step response of the head in three axes of rotation. It could be shown that linear prediction does not provide the necessary accuracy in the simulated position. A further analysis of the recorded data revealed that it is possible to take advantage of the head's latency to improve the prediction. A simple nonlinear prediction algorithm based on acceleration data was successfully implemented in the fiber optic helmet-mounted display. (Author)
Differential expression of miRNAs in response to salt stress in maize roots (2008)

Salt-responsive miRNAs are involved in the regulation of metabolic, morphological and physiological adaptations of maize seedlings at the post-transcriptional level. The miRNA genotype-specific expression model might explain the distinct salt sensitivities between maize lines.