gebhart/patrepeval huggingface dataset:
Design of Decorated Self-Assembling Peptide Hydrogels as Architecture for Mesenchymal Stem Cells (2016)

Hydrogels from self-assembling ionic complementary peptides have been receiving a lot of interest from the scientific community as mimetic of the extracellular matrix that can offer three-dimensional supports for cell growth or can become vehicles for the delivery of stem cells, drugs or bioactive proteins. In order to develop a 3D "architecture" for mesenchymal stem cells, we propose the introduction in the hydrogel of conjugates obtained by chemoselective ligation between a ionic-complementary self-assembling peptide (called EAK) and three different bioactive molecules: an adhesive sequence with 4 Glycine-Arginine-Glycine-Aspartic Acid-Serine-Proline (GRGDSP) motifs per chain, an adhesive peptide mapped on h-Vitronectin and the growth factor Insulin-like Growth Factor-1 (IGF-1). The mesenchymal stem cell adhesion assays showed a significant increase in adhesion and proliferation for the hydrogels decorated with each of the synthesized conjugates; moreover, such functionalized 3D hydrogels support cell spreading and elongation, validating the use of this class of self-assembly peptides-based material as very promising 3D model scaffolds for cell cultures, at variance of the less realistic 2D ones. Furthermore, small amplitude oscillatory shear tests showed that the presence of IGF-1-conjugate did not alter significantly the viscoelastic properties of the hydrogels even though differences were observed in the nanoscale structure of the scaffolds obtained by changing their composition, ranging from long, well-defined fibers for conjugates with adhesion sequences to the compact and dense film for the IGF-1-conjugate.
Association Between the CYP3A5 Genotype and Blood Pressure (2005)

We tested the hypothesis that the presence of a CYP3A5*1 allele is associated with increases in blood pressure in 2 studies of subjects with a total of 683 participants. The first study involving 271 subjects was part of a longitudinal study conducted at Indiana University Medical Center that consisted of 2 phases. The first phase studied the relationship of salt sensitivity with blood pressure, whereas the second phase, conducted approximately 26 years later, studied the relationship between blood pressure, carbohydrate intolerance, and vascular compliance in the same subjects. The second study was a cross-sectional evaluation of 412 normotensive and hypertensive subjects conducted at the University of California San Diego. The second study (Mantel-Haenszel chi(2) test; P=0.05) showed that a greater proportion of black participants with poor blood pressure control had CYP3A5*1/*1 genotype. Evaluation of the untreated blood pressure from phase 1 of the first study showed that the blacks with CYP3A5*3/*3 (146+/-35 mm Hg) had a higher systolic blood pressure than those with the *1/*3 (119+/-14.1 mm Hg; P=0.0006) and *1/*1 (125+/-17.4 mm Hg; P=0.009) genotypes. For blacks in study 2, the CYP3A5*1 allele was more common in hypertensives (Fisher exact test; P=0.025) than normotensives. In whites there was no association between CYP3A5 genotype and blood pressure in either study. We conclude that although untreated blood pressure may be higher in blacks with the CYP3A5*3/*3 genotype, the CYP3A5*1 allele may be associated with hypertension that is more refractory to treatment in this ethnic group.
Novel Phosphonium Chloride-Catalyzed Dehydrohalogenative Si−C Coupling Reaction of Alkyl Halides with Trichlorosilane (2001)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTNovel Phosphonium Chloride-Catalyzed Dehydrohalogenative Si−C Coupling Reaction of Alkyl Halides with TrichlorosilaneYeon Seok Cho, Seung-Hyun Kang, Joon Soo Han, Bok Ryul Yoo, and Il Nam JungView Author Information Organosilicon Chemistry Laboratory Korea Institute of Science & Technology P.O. Box 131, Cheongryang, Seoul 130-650, Korea Cite this: J. Am. Chem. Soc. 2001, 123, 23, 5584–5585Publication Date (Web):April 21, 2001Publication History Received22 November 2000Revised6 April 2001Published online21 April 2001Published inissue 1 June 2001https://pubs.acs.org/doi/10.1021/ja005814uhttps://doi.org/10.1021/ja005814urapid-communicationACS PublicationsCopyright © 2001 American Chemical SocietyRequest reuse permissionsArticle Views2232Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Alkyls,Anions,Catalysts,Chemical reactions,Coupling reactions Get e-Alerts
Low-dose ARA-C and 1(OH) D3 administration in acute non lymphoid leukemia: pilot study. (1992)

The results are in line with those showing that low doses of ARA-C are an effective treatment in this type of leukemia. In some cases (7/11), a monocytic/monoblastic shift was detected. The demonstration of 1,25(OH)2D3 receptors in some blasts is also reported. Thus it is possible to suggest that the vitamin D metabolite displays "in vivo" the differentiating activity already shown "in vitro".
MUC4 Mucin Interacts with and Stabilizes the HER2 Oncoprotein in Human Pancreatic Cancer Cells (2008)

MUC4, a high-molecular weight transmembrane glycoprotein, is overexpressed in pancreatic cancer and is implicated in its pathogenesis. It is a heterodimeric protein containing a large extracellular, heavily glycosylated subunit, MUC4alpha, and a transmembrane growth factor-like subunit, MUC4beta. In the present study, we have shown the interaction of human MUC4 with the receptor tyrosine kinase HER2 in pancreatic adenocarcinoma cells by reciprocal coimmunoprecipitation and cocapping studies. MUC4 colocalized with HER2 at the cell surface and in the cytoplasm. Silencing of MUC4 by transient or stable expression of MUC4-targeted short-interfering RNA led to the down-regulation of HER2 with a concomitant decrease in its phosphorylated form (pY(1248)-HER2). Further analyses revealed that the MUC4-knockdown-mediated decrease in HER2 expression occurred due to the drop in the stability of the receptor. In MUC4-knockdown pancreatic cancer cells, we also observed a reduced phosphorylation of the focal adhesion kinase and p42/44 mitogen-activated protein kinase, which are downstream effector proteins in HER2 signaling. Our findings add a new dimension to MUC4 function as a modulator of cell signaling and provide mechanistic evidence for its role in pancreatic cancer progression.
Synthesis of pyrido[2,3-b]indoles and pyrimidoindoles via Pd-catalyzed amidation and cyclization (2012)

Biologically and pharmaceutically active core structures containing a new class of 4-hydroxy-α-carbolines, dihydropyrido[2,3-b]indoles, pyrimido[4,5-b] and [5,4-b]indoles have been synthesized in good yields via Pd-catalyzed amidation and cyclizations. The keto-enol tautomerism in 4-hydroxy-α-carbolines has been investigated by DFT calculations and spectroscopic techniques. The fluorescence studies of pyrimido[4,5-b] and [5,4-b]indoles were carried out with good quantum yields.
Speech signal modification to increase intelligibility in noisy environments (2007)

The role of transient speech components on speech intelligibility was investigated. Speech was decomposed into two components--quasi-steady-state (QSS) and transient--using a set of time-varying filters whose center frequencies and bandwidths were controlled to identify the strongest formant components in speech. The relative energy and intelligibility of the QSS and transient components were compared to original speech. Most of the speech energy was in the QSS component, but this component had low intelligibility. The transient component had much lower energy but was almost as intelligible as the original speech, suggesting that the transient component included speech elements important to speech perception. A modified version of speech was produced by amplifying the transient component and recombining it with the original speech. The intelligibility of the modified speech in background noise was compared to that of the original speech, using a psychoacoustic procedure based on the modified rhyme protocol. Word recognition rates for the modified speech were significantly higher at low signal-to-noise ratios (SNRs), with minimal effect on intelligibility at higher SNRs. These results suggest that amplification of transient information may improve the intelligibility of speech in noise and that this improvement is more effective in severe noise conditions.
Hierarchical Nanostructures of Organosilicate Nanosheets within Self-Organized Block Copolymer Films (2008)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTHierarchical Nanostructures of Organosilicate Nanosheets within Self-Organized Block Copolymer FilmsTomoyasu Hirai†‡, Melvina Leolukman†, Teruaki Hayakawa*‡, Masa-aki Kakimoto‡, and Padma Gopalan*†View Author Information Department of Materials Science and Engineering, University of Wisconsin—Madison, Madison, Wisconsin 53706, and Department of Organic and Polymeric Materials, Tokyo Institute of Technology, 2-12-1-S8-26 O-okayama, Meguro-ku Tokyo 152-8552, Japan* Corresponding authors: Tel 608-265-4258 (P.G.), +81-3-5734-2429 (T.H.); Fax 608-262-8353 (P.G.), +81-3-5734-2875 (T.H.); e-mail [email protected] (P.G.), [email protected] (T.H.).†University of Wisconsin—Madison.‡Tokyo Institute of Technology.Cite this: Macromolecules 2008, 41, 13, 4558–4560Publication Date (Web):June 11, 2008Publication History Received19 April 2008Revised20 May 2008Published online11 June 2008Published inissue 1 July 2008https://pubs.acs.org/doi/10.1021/ma800872vhttps://doi.org/10.1021/ma800872vrapid-communicationACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views2516Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Anionic polymerization,Chemical structure,Copolymers,Transmission electron microscopy,X-ray scattering Get e-Alerts
L-type voltage-dependent calcium channel alpha-1C subunit mRNA is present in ejaculated human spermatozoa (2000)

The current study adds to the growing body of evidence that RNA is present in mature ejaculated human spermatozoa. We report that a sodium dodecyl sulphate (SDS)/citric acid extraction method is superior to guanidinium isothiocyanate in terms of reproducibility of RNA recovery from motile sperm populations from individual ejaculates. Using the SDS/citric acid method, RNA was recovered from both fresh and frozen-thawed motile spermatozoa. Sperm RNA were used as templates in reverse transcription-polymerase chain reaction (RT-PCR), in an attempt to identify partial RNA transcripts of a highly conserved region within the alpha-1C (pore-forming) subunit of L-type voltage-dependent calcium channels from 11 individual donors. Control reactions employed primers derived from the human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) sequence. In nine of the 11 specimens, gene-specific PCR products were obtained with both the GAPDH and alpha-1C primer pairs. DNA sequencing analysis confirmed that the respective spliced transcripts were amplified. The two cases in which no amplification was obtained were attributed to reduced RNA yield. These data are consistent with results from in-situ RT-PCR of rat testis sections indicating that the testis-specific calcium channel of that species was expressed uniformly in all stages of the germinal epithelium, including mature spermatozoa.
Cryo IR Spectroscopy of N<sub>2</sub> and H<sub>2</sub> on Ru<sub>8</sub><sup>+</sup>: The Effect of N<sub>2</sub> on the H-Migration (2018)

We present the cryo IR-PD spectra of the coadsorbed [Ru<sub>8</sub>(H<sub>2</sub>)<sub>1</sub>(N<sub>2</sub>)<sub>4</sub>]<sup>+</sup> and [Ru<sub>8</sub>(N<sub>2</sub>)<sub>4</sub>(H<sub>2</sub>)<sub>1</sub>]<sup>+</sup> species differing in the adsorption sequence of H<sub>2</sub> and N<sub>2</sub>, which we record via application of tandem cryo ion trapping. We observe strong evidence for dissociative H<sub>2</sub> adsorption, and the spectra reveal differences in the Ru-H stretching region, which we assign to distal and proximal hydrogen atom locations on the Ru<sub>8</sub><sup>+</sup> cluster, their migration likely hindered by preloaded nitrogen molecules and unaffected by subsequent N<sub>2</sub> adsorption.
Bio-Ethanol Based Ethylene (2009)

The use of bioethanol-based ethylene as raw material for chemical products, especially to produce polyethylene and other polymers, is being considered an effective way to capture carbon dioxide from the atmosphere and offer to the market an alternative of a commodity plastic with a wide range of applications and a low carbon footprint. The present article discloses the aspects relatives to the ethanol manufacture in Brazil using sugarcane as feedstock, the catalytic dehydration reaction, the plants operated in the past with this technology and the main characteristics of the processes.
Fabrication and Imaging of Nanometer-Sized Protein Patterns (1999)

This Letter describes a new method for production of nanometer-sized protein patterns with precise control of the size and geometry. Self-assembled monolayers (SAMs) are first patterned using nanografting, an atomic force microscopy (AFM)-based lithographic method. Methyl-terminated n-alkanethiols serve as matrix layers within which nanopatterns of thiols with carboxylate or aldehyde terminal groups are fabricated. By controlling solution conditions, we selectively immobilize proteins on the patterned areas, through either electrostatic interactions or covalent binding. In our approach, AFM tips are used for both fabrication and characterization. AFM allows patterned SAMs and proteins to be visualized in situ. The individual proteins within the nanopatterns and their orientations can be clearly resolved from the AFM images.
Coming out soon: trillion-color TV (2004)

While film used in cinema contains pigments that can create an infinitely large number of color variations, TV sets combine discrete amounts of red, green, and blue light to create a much more limited color range. To produce more colors, TV sets would have to combine purer, or more saturated, red, green, and blue by using narrowband light filters that select colors more precisely but diminish brightness at the same time. Genoa Color Technologies Ltd., an Isreali company, has developed the software and hardware necessary to close this gap and make TV images more cinemalike without the unwanted dimming side effect. The trick is to add extra primaries, the basic colors that, combined, form all others. By adding yellow and cyan to the standard red, green, and blue, a display that could normally generate about 16.7 million colors would now be able to show more than one trillion. In a demonstration showing the overall color improvement, Genoa's multiprimary system exhibited colors that current television sets cannot display.
Decreased frequency and highly aberrant spectrum of ultraviolet-induced mutations in the hprt gene of mouse fibroblasts expressing antisense RNA to DNA polymerase zeta. (2003)

In the budding yeast Saccharomyces cerevisiae, DNA polymerase zeta (pol zeta) is responsible for the great majority of mutations generated during error-prone translesion replication of DNA that contains UV-induced lesions. The catalytic subunit of pol zeta is encoded by the Rev3 gene. The orthologue of Rev3 has been cloned from higher eukaryotic cells, including human, but its role in mutagenesis and carcinogenesis remains obscure. Investigation into the cellular function of pol zeta has been hindered by the fact that Rev3 knockout mice do not survive beyond midgestation, and embryonic stem cells used to derive these mice are not genetically stable. We have generated a transgenic mouse that expresses antisense RNA transcripts to mRev3 endogeneous RNA. These mice are viable, have greatly reduced levels of Rev3 transcript, and have reduced levels of B cells and impaired development of high-affinity memory B cells. Here, we report that exposure of fibroblasts derived from these mice to UV resulted in a 4-5-fold reduction in mutant frequency at the hprt locus at every dose examined, and the mutation spectrum was highly aberrant compared with the control cells. In the control cells, 80% of the mutations were transitions and approximately 75% of these arose from photoproducts in the putative leading strand template. Strikingly, in transgenic cells, most of the mutations were transversions and there was a complete loss of strand bias. This mutation spectrum is highly aberrant and is similar to that induced by UV in human xeroderma pigmentosum variant cells, which lack polymerase eta. These data indicate that most UV-induced mutations are dependent on DNA pol zeta, a function that has been conserved from yeast to higher eukaryotic cells. However, in mammalian cells, other DNA polymerase(s) may accomplish error-prone translesion replication and are responsible for residual UV mutagenesis observed in the absence of pol zeta. Further, these data support a central role for DNA polymerase eta in the error-free bypass of UV photoproducts. The antisense Rev3 mice should be a useful model to study mutagenic lesion bypass by pol zeta in mammalian cells and to investigate the role this polymerase plays in carcinogenesis.
Promoting convergence in multi-channel blind signal separation using PNLMS (2011)

The proportionate normalized least-mean squares (PNLMS) adaptation algorithm exploits the sparse nature of acoustic impulse responses and assigns adaptation gain proportional to the absolute value of filter coefficients, thereby resulting in faster convergence. In the past it has shown to improve con vergence of acoustic paths in echo-cancellation applications. In this paper, we investigate the use of PNLMS algorithm for blind speech separation and show that with a careful selection of operating parameters the PNLMS algorithm greatly helps promote convergence of the un-mixing filters when compared to the conventional normalized least-mean-squares (NLMS) adaptation. The PNLMS based blind speech separation is suitable for real-time implementation as it promises faster con vergence and requires only a modest increase in complexity as compared to the NLMS algorithm.
Cullin 5 regulates Dab1 protein levels and neuron positioning during cortical development (2007)

Many laminated regions of the mammalian brain develop by the migration of neuronal precursor cells, whose final positions are coordinated by signals from the secreted molecule Reelin. Early events in Reelin signaling have been identified, but the mechanism of signal down-regulation has been unclear. A possible source of negative feedback is the Reelin-induced degradation of the critical intracellular signaling component, Disabled-1 (Dab1). Here we show that degradation of Dab1 depends on Dab1 phosphorylation at specific tyrosine residues and on the E3 ubiquitin ligase component Cullin 5 (Cul5). Cul5 forms complexes with SOCS (suppressors of cytokine signaling) proteins, which bind to phosphorylated Dab1 and target it for degradation in tissue culture cells. Ablation of Cul5 in migrating neurons causes an accumulation of active Dab1 protein and a unique cortical layering defect, characterized by excess migration and buildup of neurons at the top of the cortical plate. The results implicate Cul5 and SOCS proteins in down-regulation of Dab1 in vivo and show that Cul5 plays an essential role in regulating neuron migrations during cortical development, possibly by opposing a promigratory effect of Dab1.
In Vivo Studies of an Implantable Energy Convertor for Skeletal Muscle Powered Cardiac Assist (1997)

A device that harnesses the mechanical energy of skeletal muscle contracting in a linear configuration has been implanted in goats. This energy convertor transforms muscle work to hydraulic energy that could drive a variety of cardiac assist devices. The device is mounted with a rib clamp and plate affixed to the sternum by cortical bone screws. A transcutaneous hydraulic line carries a silicon based working fluid to an external system that controls the muscle load. In 60 to 70 kg goats, the latissimus dorsi insertion was reattached to the energy convertor. A Telectronics myostimulator with intramuscular electrodes stimulated the latissimus dorsi. In acute implants, hydraulic pressures in excess of 150 psi were obtained. Chronic implantation of the device allowed system evaluation in the conscious unanesthetized animal. Two weeks after implant, hydraulic pressures in excess of 200 psi were obtained and energy transferred to the external loading system exceeded 1 J per contraction. Six weeks after implant, the device continued to cycle freely. These initial results are very promising and suggest an implantable energy convertor is feasible. Development of an energy convertor is an important step toward tether-free skeletal muscle powered cardiac assist devices.
Magnetic chelating nanoprobes for enrichment and selective recovery of metalloproteases from human saliva (2014)

New magnetic nanoprobes based on surface-functionalized magnetite particles were synthesized and used to selectively capture metalloproteases from human saliva samples. In addition to their high specific surface area, these nanoparticles have metal ion chelating moieties grafted on their surface by the reaction with the organosilane EDTA-TMS ((N-(trimethoxysilylpropyl)-ethylenediaminetriacetate trisodium salt). The most distinct feature of these particles is their capability to selectively recover metalloproteases even in highly diluted saliva samples. The high affinity of these materials for metalloproteases was attributed to the formation of chelates between the chelating moieties and the metal ions of metalloproteases. These magnetic particles exhibited high colloidal stability in the biological standard buffers MES, HEPES, TEAB and Tris-HCl, within a significant pH range (pH 5-9) and, due to their magnetic features, enabled a fast separation of the metalloproteases from the medium by simply applying an external magnetic field. Thus, these materials were proven to be valuable probes for the selective enrichment and rapid recovery of metalloproteases from human saliva, particularly when dealing with trace amounts of material.