gebhart/patrepeval huggingface dataset:
SMADS AS INTRACELLULAR MEDIATORS OF AIRWAY INFLAMMATION (2004)

Transforming growth factor-beta (TGF-beta) plays an important role in the pathogenesis of allergic asthma and other airway diseases. Signals from the activated TGF-beta receptor complex are transduced to the nucleus of airway cells by Smad proteins, which represent a family of transcription factors that have recently been implicated to play a major role as intracellular mediators of inflammation. The Smad family consists of the receptor-regulated Smads, a common pathway Smad, and inhibitory Smads. Receptor-regulated Smads (R-Smads) are phosphorylated by the TGF-beta type Ireceptor. They include Smad2 and Smad3, which are recognized by TGF-beta and activin receptors, and Smads 1, 5, 8, and 9, which are recognized by bone morphogenetic protein (BMP) receptors. Smad4 is a common pathway Smad, which is also defined as cooperating Smad (co-Smad) and is not phosphorylated by the TGF-beta type I receptor. Inhibitory Smads(anti-Smads) include Smad6 and Smad7, which down-regulate TGF-beta signaling. To date, the Smads are the only TGF-beta receptor substrates with a demonstrated ability to propagate signals and with regard to the growing number of investigations of Smad-mediated effects in the airways, Smads may prove to be an important target for future development of new therapeutic strategies for asthma and chronic obstructive pulmonary disease.
Automatic Discovery of Part-Whole Relations (2006)

An important problem in knowledge discovery from text is the automatic extraction of semantic relations. This paper presents a supervised, semantically intensive, domain independent approach for the automatic detection of part-whole relations in text. First an algorithm is described that identifies lexico-syntactic patterns that encode part-whole relations. A difficulty is that these patterns also encode other semantic relations, and a learning method is necessary to discriminate whether or not a pattern contains a part-whole relation. A large set of training examples have been annotated and fed into a specialized learning system that learns classification rules. The rules are learned through an iterative semantic specialization (ISS) method applied to noun phrase constituents. Classification rules have been generated this way for different patterns such as genitives, noun compounds, and noun phrases containing prepositional phrases to extract part-whole relations from them. The applicability of these rules has been tested on a test corpus obtaining an overall average precision of 80.95% and recall of 75.91%. The results demonstrate the importance of word sense disambiguation for this task. They also demonstrate that different lexico-syntactic patterns encode different semantic information and should be treated separately in the sense that different clarification rules apply to different patterns.
Evaluating strategies and systems for content based indexing of person images on the Web (2000)

Content based indexing of multimedia has always been a challenging task. The enormity and the diversity of the multimedia content on the web adds another dimension to this challenge. In this paper, we examine ways of combining visual and textual information for content based indexing of multimedia on the web. In particular, we examine different methods of combining evidences due to face detection, Text/HTML analysis and face recognition for identifying person images. We provide experimental evaluation of the following strategies: i) Face detection on the image followed by Text/HTML analysis of the containing page; ii) face detection followed by face recognition; iii) face detection followed by a linear combination of evidences due to text/HTML analysis and face recognition; and iv) face detection followed by a Dempster-Shafer combination of evidences due to text/HTML analysis and face recognition. These strategies were implemented in an automatic web search agent named Diogenes1 and compared against some well known web image search engines. The latter includes commercial systems such as Alta Vista, Lycos and Ditto, and a research prototype, WebSEEk. We report the results of our experimental retrievals where Diogenes outperformed these search engines for celebrity image queries in terms of average precision.
Overexpression, purification and crystallization of<i>Bam</i>HI endonuclease (1991)

The type II restriction endonuclease BamHI has been expressed in E. coli, producing 100-fold more enzyme than the wild type Bacillus amyloliquefaciens H strain. This high yield has facilitated purification to homogeneity of large amounts of the enzyme, along with its crystallization in a form which diffracts to at least 1.9 A in X-ray analysis.
Formation of nuclear stress granules involves HSF2 and coincides with the nucleolar localization of Hsp70 (2003)

The heat-shock response is characterized by the activation of heat-shock transcription factor 1 (HSF1), followed by increased expression of heat-shock proteins (Hsps). The stress-induced subnuclear compartmentalization of HSF1 into nuclear stress granules has been suggested to be an important control step in the regulation of stress response and cellular homeostasis in human cells. In this study, we demonstrate that the less-well characterized HSF2 interacts physically with HSF1 and is a novel stress-responsive component of the stress granules. Based on analysis of our deletion mutants, HSF2 influences to the localization of HSF1 in stress granules. Moreover, our results indicate that the stress granules are dynamic structures and suggest that they might be regulated in an Hsp70-dependent manner. The reversible localization of Hsp70 in the nucleoli strictly coincides with the presence of HSF1 in stress granules and is dramatically suppressed in thermotolerant cells. We propose that the regulated subcellular distribution of Hsp70 is an important regulatory mechanism of HSF1-mediated heat shock response.
Dual-wavelength slightly off-axis digital holographic microscopy (2012)

We propose dual-wavelength digital holographic microscopy with a slightly off-axis configuration. The axial measurement range without phase ambiguity is extended to the micrometer range by synthesizing a beat wavelength between the two wavelengths with separation of 157 nm. Real-time measurement of the specimen is made possible by virtue of the high wavelength selectivity of the Bayer mosaic filtered color CCD camera. The principle of the method is exposed, and the practicability of the proposed configuration is demonstrated by the experimental results on a vortex phase plate and a rectangular phase step.
Direct conversion of spent fuel to High-Level-Waste (HLW) glass (1994)

The Glass Material Oxidation and Dissolution System (GMODS) is a recently invented process for the direct, single-step conversion of spent nuclear fuel (SNF) to high-level waste (HLW) glass. GMODS converts metals, ceramics, organics, and amorphous solids to glass in a single step. Conventional vitrification technology can not accept feeds containing metals or carbon. The GMODS has the potential to solve several issues associated with the disposal of various US Department of Energy (DOE) miscellaneous SNFs: (1) chemical forms unacceptable for repository disposal; (2) high cost of qualifying small quantities of particular SNFs for disposal; (3) limitations imposed by high-enriched SNF in a repository because of criticality and safeguards issues; and (4) classified design information. Conversion of such SNFs to glass eliminates these concerns. A description of the GMODS, {open_quotes}strawman{close_quotes} product criteria, experimental work to date, and product characteristics are included herein.
A Comparative Analysis of Smartphone Security Behaviors and Practices (2021)

This study on smartphone security behaviors and practices is a continuation of the work in Jones and Chin (2015), where results of a 2011 student survey at a regional comprehensive university were compared to results from a 2014 survey at the same institution. That study found that students continued to be lax in their mobile security practices. In December 2019, we again surveyed undergraduate business students to determine their current level of awareness, attitudes, and security-related practices. In this current study, we compare and contrast the results of our 2019 survey to those from our 2014 administration of the same survey instrument (Jones & Chin, 2015). Our findings help us to clearly identify the specific areas where security behaviors have improved and pinpoint those areas where necessary precautions are still lacking. We believe that our results will prove useful in developing educational programs and in providing training on proper smartphone security protocols.
Validation of HLAMatchmaker algorithm in identifying acceptable HLA mismatches for thrombocytopenic patients refractory to platelet transfusions (2008)

HLAMM (both TMM and EMM) successfully identified donors associated with good transfusion outcomes in refractory recipients and represents an acceptable method of choosing donors for refractory patients.
Group II Metabotropic Glutamate Receptors Regulate the Vulnerability to Hypoxic Brain Damage (2003)

We examined the expression of metabotropic glutamate (mGlu) receptors in species of fish that differ for their vulnerability to anoxic brain damage. Although expression of mGlu1a and mGlu5 receptors was similar in the brain of all species examined, expression of mGlu2/3 receptors was substantially higher in the brain of anoxia-tolerant species (i.e., the carp Carassius carassius and the goldfish Carassius auratus) than in the brain of species that are highly vulnerable to anoxic damage, such as the trouts Salmo trutta and Oncorhynchus mykiss. This difference was confirmed by measuring the mGlu2/3 receptor-mediated inhibition of forskolin-stimulated cAMP formation in slices prepared from the telencephalon of C. auratus and S. trutta. We exposed the goldfish C. auratus to water deprived of oxygen for 4 hr for the induction of hypoxic brain damage. Although the goldfish survived this treatment, the occurrence of apoptotic cell death could be demonstrated by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling staining and by the assessment of caspase-3 activity in different brain region. The extent of cell death was highest in the medulla oblongata, followed by the optic tectum, cerebellum, and hypothalamus. No cell death was found in the telencephalon. This regional pattern of hypoxic damage was inversely related to the expression of mGlu2/3 receptors, which was lowest in the medulla oblongata and highest in the telencephalon. Treatment of the goldfish with the brain permeant mGlu2/3 receptor antagonist LY341495 (1 mg/kg, i.p.) amplified anoxic damage throughout the brain and enabled the induction of cell death by anoxia in the telencephalon. In contrast, treatment of the goldfish with the mGlu2/3 receptor agonist LY379268 (0.5 or 1 mg/kg, i.p.) was highly protective against anoxic brain damage. Finally, exposure to the antagonist LY341495 (0.5 microm) greatly amplified the release of glutamate induced by hypoxia in slices prepared from the medulla oblongata and the telencephalon of the goldfish. We conclude that expression of mGlu2/3 receptors provides a major defensive mechanism against brain damage in anoxia-tolerant species.
Colorimetric determination of hexuronic acids in plant materials (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTColorimetric determination of hexuronic acids in plant materialsRalph W. ScottCite this: Anal. Chem. 1979, 51, 7, 936–941Publication Date (Print):June 1, 1979Publication History Published online1 May 2002Published inissue 1 June 1979https://pubs.acs.org/doi/10.1021/ac50043a036https://doi.org/10.1021/ac50043a036research-articleACS PublicationsRequest reuse permissionsArticle Views1449Altmetric-Citations471LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Bacteria Detection and Differentiation Using Impedance Flow Cytometry (2018)

Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential to accurately assess bacteria levels in drinking water in real-time. Multi frequency electrical impedance spectroscopy is used to monitor a liquid sample, while it is continuously passed through the sensor. We investigate three aspects of this sensor: First we show that the sensor is able to differentiate <i>Escherichia coli</i> (Gram-negative) bacteria from solid particles (polystyrene beads) based on an electrical response in the high frequency phase and individually enumerate the two samples. Next, we demonstrate the sensor's ability to measure the bacteria concentration by comparing the results to those obtained by the traditional CFU counting method. Last, we show the sensor's potential to distinguish between different bacteria types by detecting different signatures for <i>S. aureus</i> and <i>E. coli</i> mixed in the same sample. Our investigations show that the sensor has the potential to be extremely effective at detecting sudden bacterial contaminations found in drinking water, and eventually also identify them.
Glycoconjugate glycans as viral receptors (2005)

The carbohydrate parts of cell surface glycoproteins, glycolipids, and proteoglycans constitute receptors for many enveloped as well as non-enveloped human viruses. The majority of viral receptors of carbohydrate nature are negatively charged, including sulfated glycosaminoglycans (GAGs) or glycans containing sialic acid. Not uncommonly, virus-carbohydrate interactions are responsible for specific tissue tropism, where the affinity of influenza virus for glycans in the respiratory tract containing (a2-6)-linked sialic acid is an important example. Similarly, the number and spacing of sulfates may guide viruses to optimal GAG molecules, although this remains unproven on tissue level. A further understanding of structure and tissue distribution of carbohydrate virus receptors and their viral ligands is essential for elucidating the pathogenesis of such viruses. Also neutral glycans such as histo-blood group substances may function as virus receptors. Here, natural resistance to a given viral disease may occur in a human subpopulation due to lack of such receptors caused by deletion-mutants in critical human genes. As regards antiviral applications, the receptor-destroying enzymes, in contrast to receptor binding proteins, at the surface of, for example, influenza virus have proven to be an excellent target for intervention, which is why sialic acid analogues are now in clinical use both for prophylaxis and treatment.
Micromotion-Induced Limit to Atom-Ion Sympathetic Cooling in Paul Traps (2012)

We present, and derive analytic expressions for, a fundamental limit to the sympathetic cooling of ions in radio-frequency traps using cold atoms. The limit arises from the work done by the trap electric field during a long-range ion-atom collision and applies even to cooling by a zero-temperature atomic gas in a perfectly compensated trap. We conclude that in current experimental implementations, this collisional heating prevents access to the regimes of single-partial-wave atom-ion interaction or quantized ion motion. We determine conditions on the atom-ion mass ratio and on the trap parameters for reaching the s-wave collision regime and the trap ground state.
Capability maturity model, version 1.1 (1993)

The capability maturity model (CMM), developed to present sets of recommended practices in a number of key process areas that have been shown to enhance software-development and maintenance capability, is discussed. The CMM was designed to help developers select process-improvement strategies by determining their current process maturity and identifying the issues most critical to improving their software quality and process. The initial release of the CMM, version 1.0, was reviewed and used by the software community during 1991 and 1992. A workshop on CMM 1.0, held in April 1992, was attended by about 200 software professionals. The current version of the CMM is the result of the feedback from that workshop and ongoing feedback from the software community. The technical report that describes version 1.1. is summarised.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Dendrimeric Molecular Transporters: Synthesis and Evaluation of Tunable Polyguanidino Dendrimers that Facilitate Cellular Uptake. (2005)

Nine fluorescently labeled structurally varied polyguanidino dendrimers based on diamino acid monomeric units were individually synthesized\nin an efficient, scalable sequence using a trifluoroacetamide protecting group−perguanidinylation strategy. While the dendrimers varied\nsignificantly in their ability to enter a human lymphocyte cell line, the best transporters out-performed an oligoarginine reference standard.
Managing Cross-Layer Constraints for Interactive Mobile Multimedia∗ (2003)

Streaming multimedia content to heterogeneous handheld devices in an real environment, under power, bandwidth and load constraints is a significant design challenge, due to the various capabilities of these devices and the real-time character of the streaming workloads. A unified framework that integrates low level architectural optimizations, OS power-saving mechanisms and adaptive middleware techniques can provide significant improvements in both the system performance and user experience. In this paper, we present such an integrated framework and investigate the trade-o#s involved in managing such a distributed, real-time system, while meeting the constraints imposed by the environment and maintaining acceptable QoS levels for each client. We demonstrate how the framework, which supports tight coupling of inter-level parameters can enhance user experience on handheld devices.
Altered RECQ Helicase Expression in Sporadic Primary Colorectal Cancers (2013)

Deregulation of DNA repair enzymes occurs in cancers and may create a susceptibility to chemotherapy. Expression levels of DNA repair enzymes have been shown to predict the responsiveness of cancers to certain chemotherapeutic agents. The RECQ helicases repair damaged DNA including damage caused by topoisomerase I inhibitors, such as irinotecan. Altered expression levels of these enzymes in colorectal cancer (CRC) may influence the response of the cancers to irinotecan. Thus, we assessed RECQ helicase (WRN, BLM, RECQL, RECQL4, and RECQL5) expression in primary CRCs, matched normal colon, and CRC cell lines. We found that BLM and RECQL4 mRNA levels are significantly increased in CRC (P = .0011 and P < .0001, respectively), whereas RECQL and RECQL5 are significantly decreased (P = .0103 and P = .0029, respectively). RECQ helicase expression patterns varied between specific molecular subtypes of CRCs. The mRNA and protein expression of the majority of the RECQ helicases was closely correlated, suggesting that altered mRNA expression is the predominant mechanism for deregulated RECQ helicase expression. Immunohistochemistry localized the RECQ helicases to the nucleus. RECQ helicase expression is altered in CRC, suggesting that RECQ helicase expression has potential to identify CRCs that are susceptible to specific chemotherapeutic agents.
Immunological evaluation of CTL precursor‐oriented vaccines for advanced lung cancer patients (2003)

Recent clinical trials of peptide vaccine for cancer patients have rarely resulted in tumor regression. One of the reasons for this failure could be an insufficient induction of anti-tumor responses in these regimens, in which peptide-specific memory cytotoxic T lymphocytes (CTLs) were not measured prior to vaccination. We investigated in this study whether pre-vaccination measurement of peptide-specific CTLs can provide any advantages in lung cancer patients receiving peptide vaccination with regard to safety and immunological responses. Ten patients with advanced lung cancer received vaccination with peptides under a regimen of CTL precursor-oriented vaccination, in which pre-vaccination peripheral blood mononuclear cells (PBMCs) were at first screened for reactivity in vitro to each of 14 peptides, followed by in vivo administration of only the reactive peptides. Profiles of the vaccinated peptides varied markedly among the 10 patients. This regimen was generally well-tolerated, although local skin reactions, diarrhea, and colitis were observed in 8, 2, and 1 patient, respectively. Increased CTL responses against the immunized peptides and tumor cells were observed in the post-vaccination PBMCs from 4 of 8 and 3 of 10 patients tested, respectively. Peptide-specific IgG became detectable in post-vaccination sera in 4 of 10 patients tested, and these 4 patients had a long progression-free survival. Furthermore, the median survival time of 9 patients with non-small cell lung cancer was 668.0 +/- 164.2 days. These results encourage further development of CTL precursor-oriented peptide vaccination for lung cancer patients.
A novel Plasmodium falciparum sporozoite and liver stage antigen (SALSA) defines major B, T helper, and CTL epitopes (1996)

Abstract In the search for subunit vaccines that are able to induce the type of sterile, protective immunity achieved by irradiated sporozoites, there is increasing evidence that defense mechanisms directed at the intrahepatic stage and Ags expressed at this stage are critical. We have initiated a systematic search for such molecules and report here the identification and partial characterization of a novel Plasmodium falciparum gene encoding a 70-kDa protein, expressed in both sporozoite and liver stages (SALSA), with a vaccine potential that stems from its antigenic features. Antigenicity and immunogenicity studies were conducted in individuals exposed to malaria, in immunized mice, and in chimpanzees, using a recombinant protein and two synthetic peptides. Results show that the SALSA nonrepetitive sequence defines 1) major B cell epitopes, as shown by a high prevalence of Abs to each peptide in three African areas differing in their level of endemicity; 2) Th epitopes, as demonstrated by lymphoproliferation and IFN-γ secretion in cells from the individuals from one of the low transmission areas, as well as helper effect upon Ab secretion in mice; and 3) epitopes for cytolytic lymphocytes, demonstrated in immunized and sporozoite-challenged chimpanzees, and associated with MHC class I leukocyte Ags. The latter are of particular importance, because this is the only part of the malaria life cycle in which the parasite is located in a cell expressing class I Ags and because CD8+ lymphocytes were found to be responsible for protection in experimental models.
Prognostic Value of the Metabolic Syndrome for Long-Term Outcomes in Patients Undergoing Percutaneous Coronary Intervention (2006)

The presence of MS significantly increased the risks of subsequent cardiac events among patients who underwent PCI.