gebhart/patrepeval huggingface dataset:
Targeting N-Cadherin through Fibroblast Growth Factor Receptor-4: Distinct Pathogenetic and Therapeutic Implications (2006)

Several molecular aberrations have been implicated in the pathogenesis of pituitary tumors, but few have proven thus far to be of therapeutic value. Pituitary tumor-derived fibroblast growth factor receptor-4 (ptd-FGFR4) is an alternatively transcribed cytoplasmic isoform lacking most of the extracellular domain. This oncogene recapitulates the morphological features of human pituitary tumors in transgenic mice. To investigate the therapeutic potential of targeting ptd-FGFR4, we examined the impact of FGFR4 tyrosine kinase inhibition in xenografted mice. GH4 pituitary cells expressing ptd-FGFR4 develop into invasive tumors. Systemic treatment of mice bearing ptd-FGFR4 tumors with the FGFR-selective inhibitor PD173074 resulted in recovery of membranous N-cadherin staining and a significant reduction in tumor volume with less invasive growth behavior. Mutation of tyrosine Y754F in ptd-FGFR4 abrogated the effect of PD173074-mediated inhibition. The pivotal role of N-cadherin as a mediator of this pituitary cell growth was demonstrated by small interfering RNA mediated down-regulation, which promoted invasive growth in xenografted mice. To validate this model in primary human pituitary tumors, we examined the expression of ptd-FGFR4, N-cadherin, and clinical behavior. Loss of membranous N-cadherin correlated with cytoplasmic FGFR4 expression and with tumor invasiveness in surgically resected human pituitary tumors. Primary human pituitary tumor cells treated with PD173074 showed restoration of N-cadherin to the membrane with dephosphorylation of retinoblastoma protein. These data highlight the pathogenetic significance of N-cadherin misexpression and emphasize the importance of FGFR partnership in mediating its functions.
Matrix Metalloproteinase Inhibitors in Cancer Therapy: Turning Past Failures Into Future Successes (2018)

The matrix metalloproteinases (MMP) are a family of proteolytic enzymes that degrade multiple components of the extracellular matrix. A large body of experimental and clinical evidence has implicated MMPs in tumor invasion, neoangiogenesis, and metastasis, and therefore they represent ideal pharmacologic targets for cancer therapy. From the 1990s to early 2000s, synthetic inhibitors of MMPs (MMPI) were studied in various cancer types. Unexpectedly, despite strongly promising preclinical data, all trials were unsuccessful in reducing tumor burden or improving overall survival; in addition, MMPIs had unforeseen, severe side effects. Two main reasons can explain the failure of MMPIs in clinical trials. It has now become apparent that some MMPs have antitumor effects; therefore, the broad-spectrum MMPIs used in the initial trials might block these MMPs and result in tumor progression. In addition, although MMPs are involved in the early stages of tumor progression, MMPIs were tested in patients with advanced disease, beyond the stage when these compounds could be effective. As more specific MMPIs are now available, MMP targeting could be reconsidered for cancer therapy; however, new trials should be designed to test their antimetastatic properties in early-stage tumors, and endpoints should focus on parameters other than decreasing metastatic tumor burden. <i>Mol Cancer Ther; 17(6); 1147-55. ©2018 AACR</i>.
Aging on the input versus output side: Theoretical implications of age-linked asymmetries between detecting versus retrieving orthographic information. (1999)

This experiment tested for age-linked asymmetries predicted under Node Structure theory (NST; D. G. MacKay & D. M. Burke, 1990) between detecting versus retrieving orthographic information. Older adults detected that briefly presented words were correctly spelled (e.g., endeavor) or misspelled (e.g., endeavuor) as readily as did young adults. However, they were less able than young adults to retrieve the correctly and incorrectly spelled words that they had seen. These age-linked asymmetries were not due to educational factors, stimulus characteristics, sensory-level factors, task complexity, floor or ceiling effects, general slowing, or cohort-related activities, but they were consistent with NST predictions and with similar asymmetries in a wide range of other studies. By contrast, repetition deficits in detecting and retrieving repeated- versus unrepeated-letter misspellings (e.g., elderdly vs. elderkly) were symmetrical or equivalent in magnitude for young and older adults. Implications for a wide range of theories of cognitive aging and of repetition deficits are discussed.
Cleanroom Wipers: Particle Generation (1990)

Cleanroom wipers are essential in precision cleaning processes. Particles are to be removed by these wipers, and testing and quality control are extremely important. This presentation offers results obtained by various methods used to test particle generation from commercially available wipers. This study explores possible mechanisms of particle generation and attempts to identify a realistic testing method that simulates conditions of wiper use.
Synthesis and Use of Tris(trimethylsilyl)antimony for the Preparation of InSb Quantum Dots (2008)

A straightforward experimental procedure was developed for the synthesis of highly crystalline InSb quantum dots (QDs). Spherical QDs were obtained via transsilyation of In(O2CCH3)3 with tris(trimethylsilyl)-antimony in the presence of coordinating ligands.
Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system. (2001)

This study aims to investigate whether the immunohistochemical expression of galectin-8 could be used as a diagnostic marker in tumor tissues of various histogenetic origins including specimens from epithelial (n=145), mesenchymatous (n=16), adipous (n=10) and central and peripheral nervous system (n=25) tissue, and 4 mesotheliomas. Immunohistochemical reactions were carried out with a polyclonal anti-galectin-8 antibody and histological slides from tissues derived from the files of the Laboratory of Anatomopathology of University Erasmus Hospital, Brussels. Formalin-fixed paraffin-embedded tissues of 45 normal cases as well as 41 benign and 114 malignant tumors were studied. Marked decreases in immunohistochemical galectin-8 expression were observed in colon (p=0.001), pancreas (p=0.007), liver (p=0.0008), skin (p=0.002) and larynx (p=0.02) tissue when comparing malignant tissue to normal tissue and/or benign tumors. The reverse relationship was observed for breast tissue (p=0.007). No statistically significant differences (p>0.05) were detected when comparing normal tissue and/or benign to malignant tumors in lung, bladder, kidney, prostate and stomach tissue. Significant galectin-8 expression was also measured in non-epithelial tissue including tumors of the central and peripheral nervous system as well as in skeletal muscle and mesotheliomas. Immunohistochemical monitoring of galectin-8 thus reveals an organ-type-dependent regulation of expression upon malignant transformation of various tissue types of epithelial origin. This observation will prompt further studies to delineate any relationship with prognosis.
MEMS Technology Sensors as a More Advantageous Technique for Measuring Foot Plantar Pressure and Balance in Humans (2016)

Locomotor activities are part and parcel of daily human life. During walking or running, feet are subjected to high plantar pressure, leading sometimes to limb problems, pain, or foot ulceration. A current objective in foot plantar pressure measurements is developing sensors that are small in size, lightweight, and energy efficient, while enabling high mobility, particularly for wearable applications. Moreover, improvements in spatial resolution, accuracy, and sensitivity are of interest. Sensors with improved sensing techniques can be applied to a variety of research problems: diagnosing limb problems, footwear design, or injury prevention. This paper reviews commercially available sensors used in foot plantar pressure measurements and proposes the utilization of pressure sensors based on the MEMS (microelectromechanical systems) technique. Pressure sensors based on this technique have the capacity to measure pressure with high accuracy and linearity up to high pressure levels. Moreover, being small in size, they are highly suitable for this type of measurement. We present two MEMS sensor models and study their suitability for the intended purpose by performing several experiments. Preliminary results indicate that the sensors are indeed suitable for measuring foot plantar pressure. Importantly, by measuring pressure continuously, they can also be utilized for body balance measurements.
Transmission characteristics of dielectric-coated metallic waveguide for infrared transmission: Slab waveguide model (1983)

A metallic hollow waveguide with inner dielectric multilayers is proposed for the transmission of infrared light and its basic transmission characteristics are fully analyzed by using a two-dimensional slab waveguide model. The power loss in the waveguide is shown to be extremely reduced by coating dielectric layers properly. Simple loss formulas are also presented which consider absorptions of dielectric materials, and minimum possible losses are theoretically estimated. Finally, relations to bending losses in the waveguide are discussed.
An integrated 6DoF video camera and system design (2019)

Designing a fully integrated 360° video camera supporting 6DoF head motion parallax requires overcoming many technical hurdles, including camera placement, optical design, sensor resolution, system calibration, real-time video capture, depth reconstruction, and real-time novel view synthesis. While there is a large body of work describing various system components, such as multi-view depth estimation, our paper is the first to describe a complete, reproducible system that considers the challenges arising when designing, building, and deploying a full end-to-end 6DoF video camera and playback environment. Our system includes a computational imaging software pipeline supporting online markerless calibration, high-quality reconstruction, and real-time streaming and rendering. Most of our exposition is based on a professional 16-camera configuration, which will be commercially available to film producers. However, our software pipeline is generic and can handle a variety of camera geometries and configurations. The entire calibration and reconstruction software pipeline along with example datasets is open sourced to encourage follow-up research in high-quality 6DoF video reconstruction and rendering 1 .
Elastin synthesis by ligamentum nuchae fibroblasts: effects of culture conditions and extracellular matrix on elastin production. (1981)

Fetal bovine ligamentum nuchae fibroblasts maintained in culture synthesized soluble elastin but were unable to form the insoluble elastic fiber. Secreted elastin precursors accumulated in culture medium and were measured using a radioimmunoassay for elastin. When elastin production was examined in ligament tissue from fetal calves of various gestational ages, cells from tissue taken during the last trimester of development produced significantly more elastin than did cells from younger fetal tissue, with maximal elastin synthesis occurring shortly before birth. Soluble elastin was detected in ligament cells plated at low density until proliferation began to be density inhibited and the cells became quiescent. Also, soluble elastin production per cell declined with increasing population doubling or with age in culture. Cells grown in the presence of 5% fetal calf serum produced approximately four times as much soluble elastin as cells grown in serum-free medium. The addition of dexamethasone (0.1 microM) and bleomycin (1 microgram/ml) increased soluble elastin production by cultured cells 180% and 50%, respectively, whereas theophylline (5 micrograms/ml) depressed production 50% and antagonized stimulation by dexamethasone. Ascorbate (50 micrograms/ml), soybean trypsin inhibitor (1 mg/ml), insulin (100 microunits/ml), and aminoacetonitrile (50 micrograms/ml) had no effect, but cycloheximide at 10(-4) M completely inhibited soluble elastin production. In contrast to cells in culture, ligament tissue minces (ligament cells surrounded by in vivo extracellular matrix) efficiently incorporated soluble elastin precursors into insoluble, cross-linked elastin. In addition, soluble elastin production per cell (per microgram of DNA) was higher in tissue minces than elastin production by cells maintained on plastic. These results suggest a role for extracellular matrix in formation of the elastic fiber and in stabilizing elastin phenotypic expression by ligament fibroblasts. Fibroblasts from the bovine ligamentum nuchae present an excellent model for in vitro studies of elastin biosynthesis.
Development of green, yellow, and amber light emitting diodes using InGaN multiple quantum well structures (2007)

The authors present optical and electrical data for long wavelength (573–601nm) InGaN∕GaN multiple quantum well light emitting diodes (LEDs) grown by metal organic chemical vapor deposition. These results are achieved by optimizing the active layer growth temperature and the quantum well width. Also, the p-GaN is grown at low temperature to avoid the disintegration of the InGaN quantum wells with high InN content. A redshift is observed for both the green and yellow LEDs upon decreasing the injection current at low current regime. In the case of the yellow LED, this shift is enough to push emission into the amber (601nm).
Substrate specificity of α‐1,6‐mannosyltransferase that initiates <i>N</i>‐linked mannose outer chain elongation in <i>Saccharomyces cerevisiae</i> (1997)

Yeast Saccharomyces cerevisiae OCH1 gene encodes the mannosyltransferase that is essential for the outer chain elongation of N-linked oligosaccharides. Mannosyltransferase activity of OCH1 gene product (Och1p) was measured on HPLC by using pyridylaminated Man8GlcNAc2 (Man8GlcNAc2-PA) as an acceptor and the reaction product was observed at the retention time corresponding to Man9GlcNAc2-PA. 1H-NMR and fast atom bombardment mass spectrometry (FAB-MS) fragmentation analysis of Man9GlcNAc2-PA showed that the additional mannose was attached with an alpha-1,6 linkage at the site where mannose outer chain elongation initiates. Substrate specificity of Och1p was investigated by using various high mannose-type oligosaccharides as acceptors. Man8GlcNAc2 was the best acceptor for Och1p. The loss of one or two alpha-1,2-mannoses from Man8GlcNAc2 reduced the mannosyltransferase activity and the Man5GlcNAc2 completely lacking alpha-1,2-mannose residues did not serve as an acceptor. Man8GlcNAcOH that involves an open sugar ring by reduction of reducing terminal GlcNAc residue did not serve as an acceptor for Och1p. The loss of three mannoses at the alpha-1,6-branch also reduced the Och1p activity. These results suggest that Och1p is an initiation specific alpha-1,6-mannosyltransferase that requires the intact structure of Man8GlcNAc for efficient mannose outer chain initiation.
A nano-liposome formulation of the PARP inhibitor Talazoparib enhances treatment efficacy and modulates immune cell populations in mammary tumors of BRCA-deficient mice (2019)

Two recently approved PARP inhibitors provide an important new therapeutic option for patients with BRCA-mutated metastatic breast cancer. PARP inhibitors significantly prolong progression-free survival in patients, but conventional oral delivery of PARP inhibitors is hindered by limited bioavailability and off-target toxicities, thus compromising the therapeutic benefits and quality of life for patients. Here, we developed a new delivery system, in which the PARP inhibitor Talazoparib is encapsulated in the bilayer of a nano-liposome, to overcome these limitations. <b>Methods</b>: Nano-Talazoparib (NanoTLZ) was characterized both <i>in vitro</i> and <i>in vivo</i>. The therapeutic efficacy and toxicity of Nano-Talazoparib (NanoTLZ) were evaluated in BRCA-deficient mice. The regulation of NanoTLZ on gene transcription and immunomodulation were further investigated in spontaneous BRCA-deficient tumors. <b>Results</b>: NanoTLZ significantly (p<0.05) prolonged the overall survival of BRCA-deficient mice compared to all of the other experimental groups, including saline control, empty nanoparticles, and free Talazoparib groups (oral and i.v.). Moreover, NanoTLZ was better tolerated than treatment with free Talazoparib, with no significant weight lost or alopecia as was observed with the free drug. After 5 doses, NanoTLZ altered the expression of over 140 genes and induced DNA damage, cell cycle arrest and inhibition of cell proliferation in the tumor. In addition, NanoTLZ favorably modulated immune cell populations <i>in vivo</i> and significantly (p<0.05) decreased the percentage of myeloid derived suppressor cells in both the tumor and spleen compared to control groups. <b>Conclusions</b>: Our results demonstrate that delivering nanoformulated Talazoparib not only enhances treatment efficacy but also reduces off-target toxicities in BRCA-deficient mice; the same potential is predicted for patients with BRCA-deficient breast cancer.
Über die Darstellung und Fragmentierung von β‐Chlor‐acroleinen (1965)

Abstract Darstellung und Eigenschaften einiger β‐Alkyl‐ und β‐Aryl‐β‐chlor‐acroleine und Versuche zur Fragmentierung zu Acetylenderivaten unter verschiedenen Bedingungen werden beschrieben.
Learning to Ask Good Questions: Ranking Clarification Questions using Neural Expected Value of Perfect Information (2018)

Inquiry is fundamental to communication, and machines cannot effectively collaborate with humans unless they can ask questions. In this work, we build a neural network model for the task of ranking clarification questions. Our model is inspired by the idea of expected value of perfect information: a good question is one whose expected answer will be useful. We study this problem using data from StackExchange, a plentiful online resource in which people routinely ask clarifying questions to posts so that they can better offer assistance to the original poster. We create a dataset of clarification questions consisting of ~77K posts paired with a clarification question (and answer) from three domains of StackExchange: askubuntu, unix and superuser. We evaluate our model on 500 samples of this dataset against expert human judgments and demonstrate significant improvements over controlled baselines.
On the design of digital filters as a sum of two all-pass filters (1985)

The necessary and sufficient conditions are given for a digital filter transfer function to be implementable as a sum of two all-pass filters. The conditions are derived directly in the <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">z</tex> -plane. The class of filters satisfying these conditions is shown to be wider than the class of filters obtained via the bilinear transformation from the corresponding conventional analog filters. An example shows that the given conditions enable us to design complementary filter pairs with different numerator and denominator orders directly using magnitude squared functions. These filters compare favorably with the corresponding classical filters.
Methylated [(benzene)(1,3‐butadiene)Ru<sup>0</sup>] Derivatives as Novel MOCVD Precursors with Favorable Properties (2011)

Abstract [(Benzene)(2‐methyl‐1,3‐butadiene)Ru 0 ] ( 1 ), [(benzene)(2,3‐dimethyl‐1,3‐butadiene)Ru 0 ] ( 2 ), and [(2,3‐dimethyl‐1,3‐butadiene)(toluene)Ru 0 ] ( 3 ) are prepared and tested as new metal‐organic (MO) ruthenium precursor complexes with favorable deposition properties for the CVD of thin ruthenium films. X‐ray diffraction (XRD) studies of single crystals of the complexes are characteristic for true Ru 0 π‐complexes without molecular structure peculiarities or significant intermolecular interactions in the solid state, which can hinder undecomposed evaporation. Differential thermal analysis (DTA) and vapor pressure data qualify the compounds as almost ideal MOCVD precursors. Thin ruthenium films are deposited successfully on silicon wafers at substrate temperatures between 200 and 400 °C in a nitrogen gas atmosphere. X‐ray photoelectron spectroscopy (XPS), four‐point probe conductivity measurements, and atomic force microscopy (AFM) are used to characterize the films. All films consist of polycrystalline metallic ruthenium with a low surface roughness.
A novel approach to the design of potent bioactive peptides by incorporation of proline brackets: antiplatelet effects of Arg‐Gly‐Asp peptides (1995)

Enhancing the potency of peptides is a critical and important step in the development of peptide drugs. We have proposed that proline residues flanking protein-protein interaction sites perform a structural role in enhancing their interaction [R.M. Kini and H.J. Evans, Biochem. Biophys. Res. Commun. 212 (1995) 1115-1124]. To test this theory, we incorporated proline residues on either or both sides of the interaction site of an antiplatelet peptide, IARGDMNA and determined the inhibitory potency of the peptides in whole blood aggregation. Inclusion of one proline residue, on either the amino or carboxy terminal side of the interaction site, enhances the antiplatelet activity to approximately the same extent (1.5- to 2.5-fold). Incorporation of proline residues on both sides enhances the activity by 7- to 13-fold. This enhancement of the biological activity of the peptide is probably due to a reduction in the number of possible conformations of the peptide, without introducing the rigidity that would accompany cyclization. Incorporation of proline brackets thus provides a novel approach to the design and development of more potent peptide drugs and ligands.
Pharmacological treatment of premature ejaculation (1999)

Ejaculatory dysfunction is the most common male sexual disorder and premature ejaculation the most common presentation of ejaculatory dysfunction. Convincing data are lacking from controlled clinical studies to support sustainable long term efficacy of psychosexual counselling in the management of premature ejaculation. The pharmacological treatment of premature ejaculation is now receiving increased attention from both physicians with an interest in sexual medicine and from the pharmaceutical industry.
Influência da diferença cátion-aniônica da dieta sobre o balanço de cálcio, fósforo e magnésio em ovinos (2004)

O efeito do balanço cátion-aniônico da dieta (BCAD) no balanço macromineral (cálcio, fósforo e magnésio), no pH urinário e fecal, na concentração sérica de cálcio, fósforo e magnésio foi estudado utilizando-se 16 carneiros machos, da raça Santa Inês, por um período de 26 dias, sendo sete de adaptação. O delineamento experimental foi em blocos ao acaso, com quatro tratamentos. Para a manipulação do BCAD foram adicionados cloreto de cálcio e bicarbonato de sódio, obtendo-se os seguintes tratamentos: -12; +30; +76 e +133mEq/kg MS da ração. Não foram encontradas diferenças (P>0,10) no pH fecal, pH urinário, balanço de fósforo e magnésio. Com o aumento do BCAD houve decréscimo da absorção e retenção de cálcio, e aumento da excreção fecal. A manipulação do BCAD interferiu no metabolismo de macrominerais, principalmente no de cálcio.