gebhart/patrepeval huggingface dataset:
The Preliminary Study on the Structure of Cop Protein by CD and NMR (1999)

Cop protein is the transcription repressor protein in rolling circle replication plasmid. With antisense RNA, Cop protein controls the copy number of plasmid. Cop family proteins have been found in various plasmids. Among Cop family proteins, Cop studied in this paper consists of 55 amino acids (Mw. 6,400), and was known to have trimer structure. Since no structural facts are elucidated, we have carried out preliminary experiments aimed at the elucidation of its three dimensional structure. The secondary structure of Cop is studied by CD and NMR. To solve the aggregation of Cop at high concentration, we tested various detergents and salts. The addition of detergents and salts could not solve the aggregation problem. However, we found that concentration is important in solving the aggregation problem. We knew that 0.18mM in 50mM potassium phosphate without any other ingredients is maximum concentration not to aggregate. Wa also investigated the pH dependence of Cop protein, and knew that Cop protein is more stable in acid state. At various temperatures, 15N-1H HSQC spectra were measured in order to find the optimal experimental condition. To enhance the peak resolution, 3D NOESY-HSQC spectrum is acquired. Since there are NOE peaks in the NH-NH region, we knew that Cop protein has -helical content, which was also confirmed by CD.
ChemInform Abstract: Organosulfur Phosphorus Acid Compounds. Part 4. Fluorobenzylphosphono‐ sulfonic Acids. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Optimal wire-sizing formula under the Elmore delay model (1996)

In this paper, we consider non-uniform wire-sizing. Given a wire s e gment of length L, let f(x) be the width of the wire at position x, 0 x L . We show that the optimal wiresizing function that minimizes the Elmore delay through the wire i s f ( x ) = ae bx , where a > 0 and b > 0 are c onstants that can be c omputed i n O (1) time. In the case where lower bound (L > 0 ) and upper bound (U > 0 ) on the wire widths are given, we show that the optimal wire-sizing function f(x) is a truncated version of ae bx that can also be determined i n O (1) time. Our wire-sizing formula can be iteratively applied to optimally size the wire s e gments in a routing tree.
United Kingdom Policy: Foreign, Strategic, Economic (1950)

Journal Article United Kingdom Policy: Foreign, Strategic, Economic Get access United Kingdom Policy: Foreign, Strategic, Economic. Appreciations by Charles Webster, Ian Jacob and E. A. G. Robinson. Preface by Clement Jones. London and New York, Royal Institute of International Affairs, 1950. 101 pp. Tables, 714′​′×5′​′⁠. 4s. 75 cents. Harold Nicolson Harold Nicolson Search for other works by this author on: Oxford Academic Google Scholar International Affairs, Volume 26, Issue 4, October 1950, Page 555, https://doi.org/10.2307/2607180 Published: 01 October 1950
El papel de las escuelas en la construcción de una sociedad civil (2003)

Una escuela capaz de reconocer la diversidad y al mismo tiempo integrar diferentes grupos y miradas debe ser capaz de desarrollar iniciativas variadas. Esta posibilidad suele ser obstruida desde las mismas políticas públicas, que suelen ser uniformes, y de los modelos mentales, - construidos a través de la práctica misma -, que impiden revisar la experiencia e imaginar escuelas algo más autónomas, capaces de atender a rasgos culturales distintivos. Este trabajo se refiere a las reflexiones surgidas en el autor como miembro de un comité evaluador para otorgar empresas autogestionadas (chartered schools) en una provincia argentina. De la experiencia del jurado haciendo su tarea y de los cincuenta grupos espontáneos que presentaron propuestas, es posible aprender sobre el tipo de problemas que aparecen cuando se intenta pasar de una escuela que actúa como agencia de un sistema centralizado que toma todas las decisiones a una escuela diversa que sea expresión de diferentes propuestas sociales. Más allá del cambio de políticas parecen necesarios importantes aprendizajes colectivos para que una escuela capaz de atender a la diversidad cultural tenga lugar. Como saldo de la experiencia se señalan la variedad y riqueza de las propuestas que la sociedad puede generar cuando tiene una oportunidad más o menos creíble para hacerlo. También, dos dificultades recurrentes aún en grupos técnicamente muy aptos: la de reflexionar sobre la práctica enriqueciéndola a partir de nuevas teorías y la de definir sistemas de gobierno. A estas tres cuestiones: la variedad de propuestas y las dificultades para reconocer la propia práctica y proponer sistemas de gobierno se refiere el trabajo. Con respecto a la variedad de las propuestas, merece señalarse que si bien ésta era mucho más rica que la surge de los programas uniformes de las políticas públicas, aún parecía insuficiente para atender a la diversidad de la sociedad. Muchos de los grupos que presentaban propuestas habían recibido en los últimos tiempos buena formación docente y eran capaces de enunciar teorías que no parecían poder poner en práctica, lo que sugiere que estas dificultades no se solucionan solamente con agregados de información sino que requieren tiempo para la construcción de conocimientos colectivos a través del debate, la participación y la evaluación de los resultados. Se concluye afirmando que nuestras sociedades no son caóticas por tener muchas voces, sino porque no hay mecanismos institucionales capaces de escucharlas. Una escuela capaz de discutir sus propios problemas no solamente podría enseñar mejor a sus alumnos los contenidos disciplinares sino que configuraría un currículum donde imaginar reglas de juego para la acción colectiva, la evaluación autónoma de la experiencia y el respeto de los diferentes actores sociales, factores centrales para la constitución de una sociedad civil autónoma y plural, sean parte de la formación que la institución facilita.
Acid-Soluble Nacleotides of Colostrum, Milk, and Mammary Gland (1963)

Journal Article Acid-Soluble Nacleotides of Colostrum, Milk, and Mammary Gland Get access TETSU JOHKE TETSU JOHKE Laboratory of Physiology of Lactation, National Institute of Animal Industry, Chiba and Department of Physiological Chemistry and Nutrition, Faculty of Medicine, the University of TokyoTokyo Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 54, Issue 5, November 1963, Pages 388–397, https://doi.org/10.1093/oxfordjournals.jbchem.a127804 Published: 01 November 1963 Article history Received: 11 May 1963 Published: 01 November 1963
A novel compound from the marine bacterium<i>Bacillus pumilus</i>S6-15 inhibits biofilm formation in Gram-positive and Gram-negative species (2011)

Biofilm formation is a critical problem in nosocomial infections and in the aquaculture industries and biofilms show high resistance to antibiotics. The aim of the present study was to reveal a novel anti-biofilm compound from marine bacteria against antibiotic resistant gram-positive and gram-negative biofilms. The bacterial extract (50 μg ml(-1)) of S6-01 (Bacillus indicus = MTCC 5559) showed 80-90% biofilm inhibition against Escherichia coli, Shigella flexneri, Proteus mirabilis and S6-15 (Bacillus pumilus = MTCC 5560) showed 80-95% biofilm inhibition against all the 10 tested organisms. Furthermore, they also reduced the hydrophobicity index and extracellular polymeric substances (EPS) production. Structural elucidation of the active principle in S6-15 using GC-MS, (1)H NMR, and (13)C NMR spectral data revealed it to be 4-phenylbutanoic acid. This is the first report of 4-phenylbutanoic acid as a natural product. The purified compound (10-15 μg ml(-1)) showed potential activity against a wide range of biofilms. This study for the first time, reports a novel anti-biofilm compound from a marine bacterium with wide application in medicine and the aquaculture industry.
Studies on the mechanisms of autophagy: formation of the autophagic vacuole. (1990)

Autophagic vacuoles form within 15 min of perfusing a liver with amino acid-depleted medium. These vacuoles are bound by a "smooth" double membrane and do not contain acid phosphatase activity. In an attempt to identify the membrane source of these vacuoles, I have used morphological techniques combined with immunological probes to localize specific membrane antigens to the limiting membranes of newly formed or nascent autophagic vacuoles. Antibodies to three integral membrane proteins of the plasma membrane (CE9, HA4, and epidermal growth factor receptor) and one of the Golgi apparatus (sialyltransferase) did not label these vacuoles. Internalized epidermal growth factor and its membrane receptor were not found in nascent autophagic vacuoles but were present in lysosome-like degradative autophagic vacuoles. All these results suggested that autophagic vacuoles were not formed from plasma membrane, Golgi apparatus, or endosome constituents. Antisera prepared against integral membrane proteins (14, 25, and 40 kD) of the RER was found to label the inner and outer limiting membranes of almost all nascent autophagic vacuoles. In addition, ribophorin II was identified at the limiting membranes of many nascent autophagic vacuoles. Finally, secretory proteins, rat serum albumin and alpha 2u-globulin, were localized to the lumen of the RER and to the intramembrane space between the inner and outer membranes of some of these vacuoles. The results were consistent with the formation of autophagic vacuoles from ribosome-free regions of the RER.
The Reverse-Exchange Interconnection Network (1980)

Properties of the reverse-exchange interconnection network are used to develop a reconfiguration scheme and a two-pass structure for enhancing the efficiency of a class of multistage interconnection networks. Functional relationships among a class of multistage interconnection networks are first derived. According to the functional relationships, we propose a reconfiguration scheme which enables a network to accomplish various interconnection functions of other networks. Then the admissible permutations along with related recursive control algorithms of the reverse-exchange interconnection network are specified through a set of theorems. Using the reverse-exchange property, we also prove that the algorithms actually work. Finally, we prove that arbitrary permutations can be realized in two passes (or 2 · 1og2N switching steps where N is the network size). By taking advantage of Benes network control algorithms, a way to control the two-pass structure is also developed.
Performance Investigation on Lithium-ion Cells with Different Electrode Thicknesses for Application in Wafer-Integrated Microbatteries (2011)

Abstract not Available.
Photonically assisted generation of continuous arbitrary millimetre electromagnetic waveforms (2003)

A novel optical-to-electrical technique for the generation of continuous periodic arbitrarily shaped millimetre-wave electromagnetic waveforms at centre frequencies of 50 GHz is demonstrated. Tailored optical pulse sequences from a novel direct space-to-time pulse shaper driving a high-speed photodiode enable generation of arbitrary phase- and frequency-modulated waveforms on a cycle-by-cycle basis.
A transient calcium‐dependent chloride current in the immature Xenopus oocyte. (1983)

Ionic currents were studied in immature full-grown Xenopus oocytes using the two-micro-electrode voltage-clamp technique. Recordings of total membrane current showed a transient outward peak during depolarizations from the approximate resting voltage (-70 or -80 mV) to voltages more positive than -20 mV. The current-voltage relation for peak outward current was U-shaped, with a maximum at about 0 mV. Replacement of external Cl with methanesulphonate reversed this transient outward current to a transient inward current. Current relaxations recorded after the membrane potential was stepped to different voltages at the time of the peak showed a component that inverted at about -25 to -30 mV. This value was close to ECl as determined by measurement of the intracellular Cl ion concentration. The reversal potential for these current relaxations changed with the external Cl concentration as predicted by the Nernst relation. Replacement of external Ca with Mg, Sr or Ba, or addition of low concentrations of Ni in the presence of Ca, eliminated the transient outward current. Increasing the external Ca concentration increased the amplitude of the transient outward current without affecting the amplitude of the steady-state current. It was concluded that the outward peak in records of total membrane current represented the contribution of a transient outward current carried by Cl ions which was dependent on the entry of external Ca. It will be noted as ICl(Ca). Decay of ICl(Ca) could be described at the normal Ca concentration by a single exponential function whose time constant showed a shallow U-shaped voltage dependence. ICl(Ca) was maximally activatable by depolarizations from a holding potential of about -100 mV, but could not be activated by depolarizations from -40 mV. The amplitude of ICl(Ca) showed a large temperature dependence as compared to the steady-state current, suggesting complex control of its activation.
Input current ripple cancellation in synchronized, parallel connected critically continuous boost converters (2002)

In high power factor AC-to-DC applications, boost power converters operating on the boundary of continuous mode and discontinuous mode switch with variable frequency and draw high peak input currents. A method is presented to parallel two or more of these power converters to reduce the high peak input currents. Each power converter continues to operate on the boundary of continuous mode and discontinuous mode and maintains the benefits of zero-voltage switching.
Two independently regulated cytochromes P-450 in a Rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate (1993)

A red-pigmented coryneform bacterium, identified as Rhodococcus rhodochrous strain 116, that grew on 2-ethoxyphenol and 4-methoxybenzoate as sole carbon and energy sources was isolated. Phylogenetic analysis based on the 16S rDNA sequences indicates that the strain clusters more closely to other rhodococci than to other gram-positive organisms with a high G + C content. Each of the abovementioned growth substrates was shown to induce a distinct cytochrome P-450: cytochrome P-450RR1 was induced by 2-ethoxyphenol, and cytochrome P-450RR2 was induced by 4-methoxybenzoate. A type I difference spectrum typical of substrate binding was induced in cytochrome P-450RR1 by both 2-ethoxyphenol (KS = 4.2 +/- 0.3 microM) and 2-methoxyphenol (KS = 2.0 +/- 0.1 microM), but not 4-methoxybenzoate or 4-ethoxybenzoate. Similarly, a type I difference spectrum was induced in cytochrome P-450RR2 by both 4-methoxybenzoate (KS = 2.1 +/- 0.1 microM) and 4-ethoxybenzoate (KS = 1.6 +/- 0.1 microM), but not 2-methoxyphenol or 2-ethoxyphenol. A purified polyclonal antiserum prepared against cytochrome P-450RR1 did not cross-react with cytochrome P-450RR2, indicating that the proteins are immunologically distinct. The cytochromes appear to catalyze the O-dealkylation of their respective substrates. The respective products of the O-dealkylation are further metabolized via ortho cleavage enzymes, whose expression is also regulated by the respective aromatic ethers.
Use of a Pine Bark Extract and Antioxidant Vitamin Combination Product as Therapy for Migraine in Patients Refractory to Pharmacologic Medication (2006)

These data suggest that the antioxidant therapy used in this study may be beneficial in the treatment of migraine possibly reducing headache frequency and severity. Further clinical investigation into the efficacy of antioxidant as therapy for chronic migraine is warranted.
Anti-nutritive activities of cereal non-starch polysaccharides in broiler diets and strategies minimizing their effects (1991)

AbstractThe non-starch polysaccharides (NSPs) of cereals exhibit anti-nutritive activity when present in broiler diets. The high levels of arabinoxylans (pentosans) in rye and β-glucans in barley are responsible for the poor nutritive value of these cereals. Australian wheats vary considerably in their nutritive value with some having very low apparent metabolizable energy (AME <13 MJ/kg) values. Recent studies have shown that the low AME wheat problem is probably due to the variation in the levels of the water-soluble wheat NSPs which are predominantly pentosans. When pentosans isolated from rye or wheat are added to broiler diets, dose-dependent depressions in nutrient digestion and growth occur. Several treatments have been shown to be effective in improving the nutritive value of cereals. Soaking the cereals in water or adding NSP-degrading enzymes decreases the anti-nutritive activity of the NSP. Addition of antibiotics to diets also has been shown to increase the nutritive value of diets with high levels of NSP suggesting that the action of these materials is, at least in part, mediated by the gut microflora.Keywords:: Anti-nutritivebroilercerealdietenzymesnon-starch polysaccharidespentosans
New, High Performance, Airport Crash-Rescue Vehicles (1985)

<div class="htmlview paragraph">Airport crash rescue vehicles are required to match limit conditions of performance in regard to acceleration, mobility and load carrying capability, with a very high degree of “on-the-day” reliability to meet emergency conditions. This paper describes a unique range of vehicles, which comprises four, six, and eight wheel drive vehicles, with independent suspension and an all up weight from 12 to 28 tonnes. Installed power is from 340 to 550 horse power (255 to 413 kW) and all vehicles drive through an automatic change speed gear box to a transfer box and thence to rigidly mounted axles with controlled slip differentials. Ventilated disc brakes with servo actuation are used on all wheels and crew cabs are designed for optimum crew safety.</div>
Joint source-channel turbo decoding of entropy-coded sources (2001)

We analyze the dependencies between the variables involved in the source and channel coding chain. This analysis is carried out in the framework of Bayesian networks, which provide both an intuitive representation for the global model of the coding chain and a way of deriving joint (soft) decoding algorithms. Three sources of dependencies are involved in the chain: (1) the source model, a Markov chain of symbols; (2) the source coder model, based on a variable length code (VLC), for example a Huffman code; and (3) the channel coder, based on a convolutional error correcting code. Joint decoding relying on the hidden Markov model (HMM) of the global coding chain is intractable, except in trivial cases. We advocate instead an iterative procedure inspired from serial turbo codes, in which the three models of the coding chain are used alternately. This idea of using separately each factor of a big product model inside an iterative procedure usually requires the presence of an interleaver between successive components. We show that only one interleaver is necessary here, placed between the source coder and the channel coder. The decoding scheme we propose can be viewed as a turbo algorithm using alternately the intersymbol correlation due to the Markov source and the redundancy introduced by the channel code. The intermediary element, the source coder model, is used as a translator of soft information from the bit clock to the symbol clock.
Lithium aluminum hydride reduction of terpene sultones (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLithium aluminum hydride reduction of terpene sultonesJoseph Wolinsky and Ronald L. MarhenkeCite this: J. Org. Chem. 1975, 40, 12, 1766–1770Publication Date (Print):June 1, 1975Publication History Published online1 May 2002Published inissue 1 June 1975https://pubs.acs.org/doi/10.1021/jo00900a019https://doi.org/10.1021/jo00900a019research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of a novel InDel based molecular marker, a potential to differentiate most of the traditional Basmati from non-Basmati rice varieties (2017)

Owing to the presence of desirable characteristics like extralong and slender grains, delicious taste, superior aroma and distinct flavor, Basmati rice varieties are immensely popular are fetching higher prices in the market than non Basmati rice varieties. Basmati rice is commonly adulterated with many look alike, long grain non Basmati rice varieties. Microsatellite markers (SSRs) have proven to be the markers of choice to detect Basmati adulteration. Here, we report the development of a novel InDel based STS marker, which can differentiate most of the traditional Basmati from non-Basmati rice varieties.
Serotype-Specific Structural Differences in the Protease-Cofactor Complexes of the Dengue Virus Family (2009)

With an estimated 40% of the world population at risk, dengue poses a significant threat to human health, especially in tropical and subtropical regions. Preventative and curative efforts, such as vaccine development and drug discovery, face additional challenges due to the occurrence of four antigenically distinct serotypes of the causative dengue virus (DEN1 to -4). Complex immune responses resulting from repeat assaults by the different serotypes necessitate simultaneous targeting of all forms of the virus. One of the promising targets for drug development is the highly conserved two-component viral protease NS2B-NS3, which plays an essential role in viral replication by processing the viral precursor polyprotein into functional proteins. In this paper, we report the 2.1-A crystal structure of the DEN1 NS2B hydrophilic core (residues 49 to 95) in complex with the NS3 protease domain (residues 1 to 186) carrying an internal deletion in the N terminus (residues 11 to 20). While the overall folds within the protease core are similar to those of DEN2 and DEN4 proteases, the conformation of the cofactor NS2B is dramatically different from those of other flaviviral apoprotease structures. The differences are especially apparent within its C-terminal region, implicated in substrate binding. The structure reveals for the first time serotype-specific structural elements in the dengue virus family, with the reported alternate conformation resulting from a unique metal-binding site within the DEN1 sequence. We also report the identification of a 10-residue stretch within NS3pro that separates the substrate-binding function from the catalytic turnover rate of the enzyme. Implications for broad-spectrum drug discovery are discussed.