gebhart/patrepeval huggingface dataset:
Recombinant human interferon-<i>γ</i>. Differences in glycosylation and proteolytic processing lead to heterogeneity in batch culture (1990)

Recombinant human interferon-gamma (Hu-IFN-gamma) produced by Chinese-hamster ovary (CHO) cells was analysed by immunoprecipitation and SDS/PAGE. Up to twelve molecular-mass variants were secreted by this cell line. Three variants were recovered after enzymic removal of all N-linked oligosaccharides or when glycosylation was inhibited by tunicamycin. The presence of three polypeptide forms rather than a single form suggested that proteolytic cleavage had occurred at two sites in both the glycosylated and non-glycosylated forms. Proteolytically cleaved IFN-gamma was more prevalent in cell lysates than in the secreted glycoprotein. In common with naturally produced IFN-gamma, both fully glycosylated IFN-gamma (asparagine residues 28 and 100 occupied) and partially glycosylated product (thought to be substituted at position Asn28) were secreted. This was deduced from the Mr of the glycosylated products and the relative amounts of sialic acid expressed by each variant. In contrast with naturally produced IFN-gamma, non-glycosylated IFN-gamma was also secreted by the transfected CHO cells. When the cells were grown in batch culture in serum-free medium under pH and dissolved-oxygen control, the proportion of non-glycosylated IFN-gamma increased from 3 to 5% after 3 h, to 30% of the total IFN-gamma present after 195 h. This change in the proportion of glycosylated protein produced was not seen when metabolically labelled IFN-gamma was incubated for 96 h with cell-free supernatant from actively growing CHO cells. This implied that an alteration in intracellular glycosylation was occurring rather than a degradation of oligosaccharide side chains after secretion. The decrease in IFN-gamma glycosylation was independent of the glucose concentration in the culture medium, but could be related to specific growth and IFN-gamma production rates, as these declined steadily after 50 h of culture, in line with the increased production of non-glycosylated IFN-gamma.
A new combinatorial algorithm for QRS detection (2013)

QRS detection is the most important step for analyzing electrocardiogram (ECG) signals. In this paper, a new combinatory algorithm based on Pan-Tompkins' method and wavelet transform is proposed to keep their strengths and avoid the weakpoints. It consists of two stages: preprocessing and decision. In the preprocessing stage, the wavelet transform is used to remove undesired details of the ECG signal and amplify QRS complexes. Then, the local energy of the signal is computed by using a Gaussian kernel. Finally, in the decision stage, similar to Pan-Tompkins' method, an adaptive threshold selection algorithm is employed to detect QRS complexes. Experimental results on the MIT-BIH database demonstrated outstanding solution quality of the proposed algorithm compared to a number of QRS detection algorithms. Furthermore, interesting properties such as low computational burden and straightforward implementation nominate the proposed algorithm as an appropriate candidate for real-time QRS detection applications.
Micro-thermal analysis of polymers: current capabilities and future prospects (2001)

The current state of development of micro-thermal analysis (micro-TA) and related techniques are briefly reviewed. Results for a PET/epoxy resin composite and a bilayer polymer film are given as illustrations. Details are given of a new interface that enables the micro-TA unit to be placed inside a conventional FTIR spectrometer to carry out photothermal IR microscopy. New results are presented for a micro-pyrolysis-mass spectroscopy technique. The limitations of the current instrumentation are discussed in terms of the overriding problem being one of spatial resolution. Images obtained using pulsed force mode AFM with a high-resolution heated tip indicate the scope for future development of this technique. The possibility of even higher spatial resolution with other forms of probe are discussed along with the potential for imaging micro-pyrolysis time of flight mass spectroscopy and even tomography. It is concluded that these methods offer excellent prospects for characterising a wide range of polymer systems.
Hypertrophy and functional alterations in hyperdynamic phospholamban-knockout mouse hearts under chronic aortic stenosis (2002)

The hyperdynamic phospholamban-knockout hearts are able to compensate against a sustained aortic stenosis similar to wild-types.
A Metal‐Metal Bonded Dinuclear Ruthenium Ketene Complex (1985)

Abstract We report the synthesis of metal‐metal bonded dinuclear ruthenium ketene complex [CpRu(CO)] 2 (μ‐CH 2 CO)(μ‐CO), 3 , (Cp=η 5 ‐C 5 H 5 ) which contains the unique structural feature, of a μ‐η 2 (C,C) ketene group bridging two metal centers. Complex 3 is prepared by a rare intramolecular CO insertion to the methylene group of a Ru complex [CpRu(CO) 2 ] 2 (μ‐CH 2 ), 1 , in the absence of CO or any donor ligand. The cis and trans isomers of 3 originating from the arrangement of the Cp groups and the unsymmetrically bridging ketene group, are observed in the NMR spectra. Unlike a ketene complex without a metal‐metal bond [CpRu(CO) 2 ] 2 (μ‐CH 2 CO), 2 , complex 3 exhibits the chemistry of a ketene molecule and might be used as a stoichiometric ketene precusor in organic reactions.
The Influence of Residence Hall Community on Academic Success of Male and Female Undergraduate Students. (2004)

Presents information on a study on the influences of gender and residence hall living on academic success of male and female undergraduate students in the U.S. Difference in patterns of involvement and interaction with peers and with the environment in their college community among males and females; Use of factor analysis and regression model in the study; Significance of self-assessment of academic progress in academic success.
Efficient Rescue of Gutted Adenovirus Genomes Allows Rapid Production of Concentrated Stocks Without Negative Selection (2002)

Gutted adenoviral (Ad) vectors have a greater cloning capacity and elicit less immune response than conventional Ad vectors. Unfortunately, clinical use of gutted vectors has been slowed by production difficulties, including low yield and a tendency for recombinant virus to emerge. These two problems are related, because expansion of dilute vector stocks requires selective pressure against helper virus. The ability to rescue gutted virus at high titer would lessen the requirement for selective pressure, thereby limiting the advantage afforded to undesirable recombinants. We tested gutted virus rescue from plasmids and from synthetic terminal protein (TP)-DNA complexes by transfection/infection or cotransfection with various forms of helper viral DNA. Optimal rescue required cotransfection of gutted and helper genomes with identical origins of replication. Transfection/infection, which introduces unequal origins, was 30 times less efficient than cotransfection of genomes that had been released from plasmid DNA and bore identical origins. Cotransfection of TP-linked genomes was several times more efficient than that of unlinked genomes, yielding average gutted viral titers above 10(7) transducing units (TU)/ml. In addition, we found that limited expression of Cre recombinase doubled the yield of gutted virus. Using these techniques, gutted viruses can be rescued at titers greater than 3 x 10(7) TU/ml, about 100 times higher than is usually achieved. Finally, we found that high-titer lysates could be serially passaged on Cre-negative cells without loss of titer, further reducing selective pressure. These methods allow large-scale production of gutted virus in three or four serial passages, while minimizing exposure to Cre recombinase.
Association Between Adoption of Evidence-Based Treatment and Survival for Patients With ST-Elevation Myocardial Infarction (2011)

In a Swedish registry of patients with STEMI, between 1996 and 2007, there was an increase in the prevalence of evidence-based treatments. During this same time, there was a decrease in 30-day and 1-year mortality that was sustained during long-term follow-up.
REVIEWS (1980)

REVIEWS Get access The Prefaces to the Waverley Novels. Edited by MARK A. WEINSTEIN. Pp. xviii+270. Lincoln and London: University of Nebraska Press, 1978. £8.40 net. DONALD A. LOW DONALD A. LOW Search for other works by this author on: Oxford Academic Google Scholar The Review of English Studies, Volume XXXI, Issue 121, 1980, Pages 90–91, https://doi.org/10.1093/res/XXXI.121.90 Published: 01 January 1980
[Value of the neuropsychological evaluation in cerebral arterial aneurysms surgically treated]. (1991)

Early examination (3 months) of 81 patients, operated on for ruptured cerebral aneurysms, by neurosurgeons and neuropsychologists show significant differences between them both; the neuropsychological evaluation has the best predictive value as far as the outcome is concerned especially regarding the return to their initial job (69% after a year in this series). The outcome also depends on the age, the preoperative grade, and the professional qualification. The aneurysm location and the date of the operation are not so important. Early neuropsychological evaluation enable us to start a rehabilitation program for socio professional reinsertion and to improve the quality of life.
Nav1.1 haploinsufficiency in excitatory neurons ameliorates seizure-associated sudden death in a mouse model of Dravet syndrome (2013)

Dravet syndrome is a severe epileptic encephalopathy mainly caused by heterozygous mutations in the SCN1A gene encoding a voltage-gated sodium channel Nav1.1. We previously reported dense localization of Nav1.1 in parvalbumin (PV)-positive inhibitory interneurons in mice and abnormal firing of those neurons in Nav1.1-deficient mice. In the present study, we investigated the physiologic consequence of selective Nav1.1 deletion in mouse global inhibitory neurons, forebrain excitatory neurons or PV cells, using vesicular GABA transporter (VGAT)-Cre, empty spiracles homolog 1 (Emx1)-Cre or PV-Cre recombinase drivers. We show that selective Nav1.1 deletion using VGAT-Cre causes epileptic seizures and premature death that are unexpectedly more severe than those observed in constitutive Nav1.1-deficient mice. Nav1.1 deletion using Emx1-Cre does not cause any noticeable abnormalities in mice; however, the severe lethality observed with VGAT-Cre-driven Nav1.1 deletion is rescued by additional Nav1.1 deletion using Emx1-Cre. In addition to predominant expression in PV interneurons, we detected Nav1.1 in subpopulations of excitatory neurons, including entorhino-hippocampal projection neurons, a subpopulation of neocortical layer V excitatory neurons, and thalamo-cortical projection neurons. We further show that even minimal selective Nav1.1 deletion, using PV-Cre, is sufficient to cause spontaneous epileptic seizures and ataxia in mice. Overall, our results indicate that functional impairment of PV inhibitory neurons with Nav1.1 haploinsufficiency contributes to the epileptic pathology of Dravet syndrome, and show for the first time that Nav1.1 haploinsufficiency in excitatory neurons has an ameliorating effect on the pathology.
Functional Analysis of the ComK Protein of Bacillus coagulans (2013)

The genes for DNA uptake and recombination in Bacilli are commonly regulated by the transcriptional factor ComK. We have identified a ComK homologue in Bacillus coagulans, an industrial relevant organism that is recalcitrant for transformation. Introduction of B. coagulans comK gene under its own promoter region into Bacillus subtilis comK strain results in low transcriptional induction of the late competence gene comGA, but lacking bistable expression. The promoter regions of B. coagulans comK and the comGA genes are recognized in B. subtilis and expression from these promoters is activated by B. subtilis ComK. Purified ComK protein of B. coagulans showed DNA-binding ability in gel retardation assays with B. subtilis- and B. coagulans-derived probes. These experiments suggest that the function of B. coagulans ComK is similar to that of ComK of B. subtilis. When its own comK is overexpressed in B. coagulans the comGA gene expression increases 40-fold, while the expression of another late competence gene, comC is not elevated and no reproducible DNA-uptake could be observed under these conditions. Our results demonstrate that B. coagulans ComK can recognize several B.subtilis comK-responsive elements, and vice versa, but indicate that the activation of the transcription of complete sets of genes coding for a putative DNA uptake apparatus in B. coagulans might differ from that of B. subtilis.
A distributed proxy server system for wireless mobile Web service (2002)

The transcoding proxy strategy has been widely used as a means to reduce the delay in retrieving Web pages over wireless mobile data networks. However, this strategy has a serious drawback of being a potential point of failure or a bottleneck of the service. We developed a distributed proxy server system in which multiple proxy servers are installed at geographically dispersed locations and share the workloads among themselves by serving mobile hosts within assigned regions. A new handoff (HO) message protocol is proposed to enable handoffs between proxies as the mobile hosts move between regions. With this proxy server handoff protocol a client agent at the mobile host requests a proxy server to initiate handoff processing and two proxy servers synchronize distilled data for a HTTP session disconnected by the handoff.
Optical parametric amplification in periodically poled KTiOPO_4 seeded by an Er–Yb:glass microchip laser (2001)

An optical parametric amplifier based on periodically poled KTiOPO(4) was used to generate 3-ns pulses at 1.544 mum . The device was pumped by a Q-switched Nd:YAG laser, and the signal output-pulse energy reached 71 muJ ; the maximum gain was 66 dB. The seed source was an Er-Yb:glass microchip laser. A theoretical fit to experimental data gave an effective nonlinear coefficient of 9.7 pm/V , close to the highest values reported for periodically poled KTiOPO(4) . Furthermore, the broad parametric gain observed could be used for broadband pulse amplification.
Toward a Source Coding Theory for Sets (2006)

The problem of communicating (unordered) sets, rather than (ordered) sequences is formulated. Elementary results in all major branches of source coding theory, including lossless coding, high-rate and low-rate quantization, and rate distortion theory are presented. In certain scenarios, rate savings of log n! bits for sets of size n are obtained. Asymptotically in the set size, the entropy rate is zero and for sources with an ordered parent alphabet, the (0,0) point is the rate distortion function.
Animal Models of GWAS-Identified Type 2 Diabetes Genes (2013)

More than 65 loci, encoding up to 500 different genes, have been implicated by genome-wide association studies (GWAS) as conferring an increased risk of developing type 2 diabetes (T2D). Whilst mouse models have in the past been central to understanding the mechanisms through which more penetrant risk genes for T2D, for example, those responsible for neonatal or maturity-onset diabetes of the young, only a few of those identified by GWAS, notably TCF7L2 and ZnT8/SLC30A8, have to date been examined in mouse models. We discuss here the animal models available for the latter genes and provide perspectives for future, higher throughput approaches towards efficiently mining the information provided by human genetics.
Robot Motion Trajectory-Measurement with Linear Inertial Sensors (2005)

The previous investigations have shown that it is possible to measure dynamic trajectory properties of industrial robots with gyro-less inertial navigation systems. As typical for INS, there are certain sources of errors which distort the overall analysis results to a certain extent. The proposed measurement principle is especially very sensitive to misalignment of the transducers in the sensor head and to bias errors of the accelerometers. With redundant configurations (e. g. a higher number of transducers than spatial degrees of freedom) there are certain possibilities to improve the overall accuracy by means of complementary filters and Kalman filters. Further research should investigate the use of low-cost gyroscopes as a source of additional motion information. One interesting â€œcandidateâ€ would be a piezoelectric vibration type gyro, like the Murata GyrostrarÂ®. Utilizing hybrid, highly-redundant sensor heads made from low-cost components could be a feasible solution for highaccurate trajectory analysis heads for affordable prices.
Characterization of the porosity developed in a new titania-alumina catalyst support prepared by the sol gel route (2003)

Titanium oxide (TiO 2 ) is a good candidate for support of hydrotreating catalysts but has the disadvantage of presenting a low surface area and a poor thermal stability when compared with Al 2 O 3 . A mixed TiO 2 -Al 2 O 3 support was proposed as an alternative that is expected to be free from these drawbacks. The variation during firing of the nanoporous texture of supports composed of TiO 2 -Al 2 O 3 , TiO 2 and Al 2 O 3 was studied by small-angle X-ray scattering (SAXS). The supports were prepared by the sol-gel route using Ti and Al isopropoxides. We have particularly analyzed the effects of acid and basic hydrolysis on the nanostructural features of catalyst supports fired at different temperatures. The nanopore radius distribution functions were determined from SAXS results assuming a simple model of spherical nanopores embedded in a homogeneous solid matrix. The modal pore radius in both pure TiO 2 and pure Al 2 O 3 supports grows from 1.3 to 2.2 nm as the firing temperature increases from 673 to 973 K. On the other hand, the modal pore radius in the mixed TiO 2 -Al 2 O 3 support remains below 1.2 nm over the same range of firing temperatures. These results demonstrate the good thermal stability of the nanoporous texture of mixed TiO 2 -Al 2 O 3 supports.
The Dual Role of HLA-G in Cancer (2014)

We here review the current data on the role of HLA-G in cancer based on recent findings of an unexpected antitumor activity of HLA-G in hematological malignancies. For the past decade, HLA-G has been described as a tumor-escape mechanism favoring cancer progression, and blocking strategies have been proposed to counteract it. Aside from these numerous studies on solid tumors, recent data showed that HLA-G inhibits the proliferation of malignant B cells due to the interaction between HLA-G and its receptor ILT2, which mediates negative signaling on B cell proliferation. These results led to the conjecture that, according to the malignant cell type, HLA-G should be blocked or conversely induced to counteract tumor progression. In this context, we will here present (i) the dual role of HLA-G in solid and liquid tumors with special emphasis on (ii) the HLA-G active structures and their related ILT2 and ILT4 receptors and (iii) the current knowledge on regulatory mechanisms of HLA-G expression in tumors.
Cognitive tests for the detection of mild cognitive impairment (MCI), the prodromal stage of dementia: Meta‐analysis of diagnostic accuracy studies (2018)

Multiple cognitive tests have comparable diagnostic accuracy. The Memory Alteration Test is short and has the highest sensitivity. New cognitive tests for MCI diagnosis should not be compared with the MMSE.
Formation of Nanometer‐Size Silicon Particles in a Laser Induced Plasma in SiH<sub>4</sub> (1993)

Abstract Crystalline silicon particles of cubic structure are formed in the plasma which is initiated in an argon‐silane mixture by a focussed 694 nm Ruby laser flash. Part of the particles can be dissolved as colloid in organic solvents. The solubilization is increased by small amounts of 1,3‐propane‐dithiol. The raw material has a broad size distribution. Centrifugation and filtration methods are applied to obtain samples with narrow size distribution. Small silicon particles have structured absorption spectra. The particles luminesce after etching with HF under air. The larger particles have a red luminescence (peaking at 720 nm) and the smaller particles emit a blue luminesce. The luminescence effects are explained by size quantization of the electronic energy levels in the particles.
Constant geometry fast Fourier transforms on array processors (1993)

Matrix algebra is used to design and validate parallel algorithms for large constant-geometry fast Fourier transforms (FFTs) on fixed-size array processors. The N-point radix 2 case for a linear array processor with N/2 cells is identical to the usual procedure corresponding to the matrix factorization of M.C. Pease, (1968). The algorithms are engendered by matrix factorizations, which themselves depend on a basic factorization of the perfect shuffle. The resulting data movement is realized in parallel as relatively small perfect shuffles inside each local memory and along each row and column of the array processor, without requiring that the complete array itself have the shuffle-exchange network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Structure and Properties of Bright Tin-Nickel Alloy Films Formed by Thermal Diffusion Method. (1994)

After nickel plating on copper substrates and tin plating on top of that, the effects of heat treatment on crystal structure, contact resistance and hardness of the double plating films were investigated. X-ray diffraction measurements showed that heating the tin /nickel double plating films at 180 for one hour resulted in the formation of tinnickel intermetallic compounds.
The Effect of Automated Underwriting on the Profitability of Mortgage Securitization (1997)

Over the past two years, many mortgage market analysts have praised automated underwriting as a technological innovation that will lower the costs of processing mortgage applications. However, automated underwriting is unlikely to decrease processing costs uniformly for all mortgage applications. Instead, it makes identifying and processing low-risk mortgage borrowers less costly, but may not significantly lower the costs of identifying and processing relatively high-risk applicants. Our results suggest that after the one-time cost reduction produced by automated underwriting, the resulting mortgage market equilibrium is characterized by lower mortgage rates and lower profits for the mortgage securitizer.
Metal-Triggered Radial Self-Assembly of Collagen Peptide Fibers (2008)

A metal-triggered self-assembling collagen peptide was designed and synthesized to generate fibers through a radial growth mechanism. The assembly of the fibers was made possible through the placement of a bipyridine ligands within the center of the triple helix and was triggered by the addition of Fe(II).
Cellular localisation of human epidermal growth factor receptor (1984)

We show here, using immunohistological techniques and a monoclonal antibody to the receptor for epidermal growth factor (EGFR) (Waterfield et al. 1982) that EGFR is present on a wide range of normal epithelial tissues and tumours arising from those sites. The distribution of the receptor suggests that EGF may be involved in the control of proliferation and possibly differentiation of surface epithelia. The strong tumour cell staining suggests an increased expression of the receptor in certain carcinomas.