gebhart/patrepeval huggingface dataset:
Renin Regulation in Cultured Proximal Tubular Cells (1996)

Recent studies have documented the presence of a complete renin-angiotensin system in the proximal tubule of the kidney: however, little is known about the regulation of renin in this proximal tubular system. Therefore, we performed the present studies to learn whether the behavior of the renin system in cultured proximal tubule is similar to that of the juxtaglomerular renin system. Basal renin secretion from rabbit proximal tubular cells in primary culture was low and not affected by isoproterenol (10(-5) mol/L), diltiazem (10(-5) mol/L), or a zero-calcium bath (O nmol/L). Only the calcium ionophore A23187 (10(-4) mol/L) significantly reduced renin secretion in these cells (from 2.44 +/- 0.37 to 1.14 +/- O.08 ng angiotensin I/mg protein per hour, P<.05). When the proximal tubular cells were lysed so the effects of the test agents on intracellular renin content could be assessed, isoproterenol caused a significant twofold (107 percent) increase (from 2.02 +/- 0.56 to 4.18 +/- 0.81 ng angiotensin I/mg protein per hour, P<.05), whereas diltiazem, A23187, and zero- and high-calcium baths did not produce a significant change. The effects of these agents on renin mRNA were examined in rabbit and rat proximal tubular cells in primary culture with the use of an S1 nuclease protection assay. Densitometry analysis of renin mRNA and either GAPDH mRNA (rat) or alpha-actin (rabbit) showed no significant alterations in renin mRNA abundance. In summary, these results confirm the presence of renin mRNA in cultured proximal tubular cells and suggest that a low-level, constitutive secretion of renin occurs in this system that is decreased by A23187. Moreover, the results also suggest that proximal tubular renin is regulated, albeit differently from the juxtaglomerular renin system. Finally, short-term increments in proximal tubular renin occur without a change in renin mRNA.
Communicating everyday experiences (2004)

In this paper, we present our approach to the problem of communicating everyday experiences. This is a challenging problem, since media from everyday events are unstructured, and often poorly annotated. We first attempt to communicate everyday experiences using a dramatic framework, by categorizing media and by introducing causal relations. Based on our experience of the dramatic framework for the everyday media, we introduce an event based framework as well as a viewpoint centric visualization that allows the viewer to have agency, in a highly interactive, non-linear manner. Our approach focuses on structured interaction for consumption of everyday experiences, in contrast to non-interactive consumption of structured communication. Our results indicate that dramatic structures do not work well with everyday media, and novel interactions / visualizations are needed. Experimental results indicate that the viewpoint centric visualization works well. We are in the process of creating a large event database of everyday events, and we are creating the necessary recording and annotation tools.
Kinetics of cytokine expression during primary human immunodeficiency virus type 1 infection. (1996)

In the present study, we have determined the kinetics of constitutive expression of a panel of cytokines [interleukin (IL) 2, IL-4, IL-6, IL-10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha)] in sequential peripheral blood mononuclear cell samples from nine individuals with primary human immunodeficiency virus infection. Expression of IL-2 and IL-4 was barely detected in peripheral blood mononuclear cells. However, substantial levels of IL-2 expression were found in mononuclear cells isolated from lymph node. Expression of IL-6 was detected in only three of nine patients, and IL-6 expression was observed when transition from the acute to the chronic phase had already occurred. Expression of IL-10 and TNF-alpha was consistently observed in all patients tested, and levels of both cytokines were either stable or progressively increased over time. Similar to IL-10 and TNF-alpha, IFN-gamma expression was detected in all patients; however, in five of nine patients, IFN-gamma expression peaked very early during primary infection. The early peak in IFN-gamma expression coincided with oligoclonal expansions of CD8+ T cells in five of six patients, and CD8+ T cells mostly accounted for the expression of this cytokine. These results indicate that high levels of expression of proinflammatory cytokines are associated with primary infection and that the cytokine response during this phase of infection is strongly influenced by oligoclonal expansions of CD8+ T cells.
Middleware support for global access to integrated computational collaboratories (2002)

The growth of the Internet and the advent of the computational "Grid" have made it possible to develop and deploy advanced computational collaboratories. These systems build on high-end computational resources and communication technologies underlying the Grid, and provide seamless and collaborative access to particular resources, services or applications. Integrating these "focused" collaboratories presents significant challenges. Key among these is the design and development of robust middleware support that addresses scalability, service discovery, security and access control, and interaction and collaboration management for consistent access. The authors first investigate the architecture of such a middleware that enables global (Web-based) access to collaboratories. They then present the design and implementation of a middleware substrate that enables a peer-to-peer integration of and global (collaborative) access to geographically distributed instances of the DISCOVER computational collaboratory for interaction and steering.
The Perils of Pathogen Discovery: Origin of a Novel Parvovirus-Like Hybrid Genome Traced to Nucleic Acid Extraction Spin Columns (2013)

Next-generation sequencing was used for discovery and de novo assembly of a novel, highly divergent DNA virus at the interface between the Parvoviridae and Circoviridae. The virus, provisionally named parvovirus-like hybrid virus (PHV), is nearly identical by sequence to another DNA virus, NIH-CQV, previously detected in Chinese patients with seronegative (non-A-E) hepatitis. Although we initially detected PHV in a wide range of clinical samples, with all strains sharing ∼99% nucleotide and amino acid identity with each other and with NIH-CQV, the exact origin of the virus was eventually traced to contaminated silica-binding spin columns used for nucleic acid extraction. Definitive confirmation of the origin of PHV, and presumably NIH-CQV, was obtained by in-depth analyses of water eluted through contaminated spin columns. Analysis of environmental metagenome libraries detected PHV sequences in coastal marine waters of North America, suggesting that a potential association between PHV and diatoms (algae) that generate the silica matrix used in the spin columns may have resulted in inadvertent viral contamination during manufacture. The confirmation of PHV/NIH-CQV as laboratory reagent contaminants and not bona fide infectious agents of humans underscores the rigorous approach needed to establish the validity of new viral genomes discovered by next-generation sequencing.
Novel N-Terminal Variant of Human VDR (2001)

The importance of N-terminal regions of nuclear hormone receptors in transcriptional regulation is increasingly recognized. As variant VDR gene transcripts indicated possible N-terminally extended receptors, we investigated their natural occurrence, transactivation capacity, and subcellular localization. A novel 54-kDa VDRB1 protein, in addition to the previously recognized 48-kDa VDRA form, was detected in human kidney tissue as well as in osteoblastic (MG63), intestinal (Int-407, DLD-1, and COLO 206F), and kidney epithelial (786) human cell lines by Western blots using isoform-specific and nonselective anti-VDR antibodies. VDRB1 was present at approximately one-third the level of VDRA. Isoform-specific VDRB1 expression constructs produced lower ligand-dependent transactivation than VDRA when transiently transfected with a vitamin D-responsive promoter into cell lines with low endogenous VDR. Intracellular localization patterns of the green fluorescent protein-tagged VDR isoforms differed. VDRB1 appeared as discrete intranuclear foci in the absence of 1,25-dihydroxyvitamin D3, whereas VDRA produced diffuse nuclear fluorescence. After 1,25-dihydroxyvitamin D3 treatment, both VDR isoforms exhibited similar diffuse nuclear signal. In the absence of 1,25-dihydroxyvitamin D3, the VDRB1 foci partially colocalized with SC-35 speckles and a subset of promyelocytic leukemia nuclear bodies. These data provide the first evidence of VDRB1, a novel N-terminally variant human VDR that is expressed at a level comparable to VDRA in human tissue and cell lines. It is characterized by reduced transactivation activity and a ligand-responsive speckled intranuclear localization. The intranuclear compartmentalization and altered functional activity of VDRB1 may mediate a specialized physiological role for this receptor isoform.
Young measures and the absence of fine microstructures in a class of phase transitions (1992)

It is shown that for a class of pairs of energy wells the only Young measures having these wells as support must reduce to spatially constant Dirac masses. This implies the prediction that fine structures will be absent in certain crystal phase transitions.
Low-noise amplifiers-then and now (2002)

The evolution in the performance of low-noise amplifiers (LNAs) has been dynamic over the past years. From the early LNAs that were complex, large, and heavy, to the present day InP high electron-mobility transistors that have virtually transformed the industry by their performance and extension into frequency bands that were not even considered in the past. This paper summarizes the transformation that has occurred in the LNA field, viewing where they were in the past, and where they are now.
The Photocycle of a Flavin-binding Domain of the Blue Light Photoreceptor Phototropin (2001)

The plant blue light receptor, phot1, a member of the phototropin family, is a plasma membrane-associated flavoprotein that contains two ( approximately 110 amino acids) flavin-binding domains, LOV1 and LOV2, within its N terminus and a typical serine-threonine protein kinase domain at its C terminus. The LOV (light, oxygen, and voltage) domains belong to the PAS domain superfamily of sensor proteins. In response to blue light, phototropins undergo autophosphorylation. E. coli-expressed LOV domains bind riboflavin-5'-monophosphate, are photochemically active, and have major absorption peaks at 360 and 450 nm, with the 450 nm peak having vibronic structure at 425 and 475 nm. These spectral features correspond to the action spectrum for phototropism in higher plants. Blue light excitation of the LOV2 domain generates, in less than 30 ns, a transient approximately 660 nm-absorbing species that spectroscopically resembles a flavin triplet state. This putative triplet state subsequently decays with a 4-micros time constant into a 390 nm-absorbing metastable form. The LOV2 domain (450 nm) recovers spontaneously with half-times of approximately 50 s. It has been shown that the metastable species is likely a flavin-cysteine (Cys(39) thiol) adduct at the flavin C(4a) position. A LOV2C39A mutant generates the early photoproduct but not the adduct. Titrations of LOV2 using chromophore fluorescence as an indicator suggest that Cys(39) exists as a thiolate.
External control of transgene expression in tobacco plastids using the bacterial <i>lac</i> repressor (2005)

Although several induction systems have been described for plants containing transgenes in the nucleus, to date there is only one method for controlling transgene expression in plastids. This consists of chemical induction of a nuclear gene and import of the gene product into plastids, so that transformation of two cellular compartments is required. Here we describe a system for external control of plastid gene expression which is based entirely on plastid components and can therefore be established in a single transformation step. Our system uses modified promoters containing binding sites for the bacterial lac repressor. Chemical induction can be made with intact plants or after harvesting, which provides ecological and economic benefits.
Influence of cholesterol oxides on endocytosis of cultured endothelial and smooth muscle cells. (1990)

Human umbilical vein endothelial cells and rabbit aortic smooth muscle cells in culture were incubated for intervals up to 24 hrs with varying concentrations of cholesterol, 7-ketocholesterol, 25-hydroxycholesterol, cholestane-3 beta,5 alpha,6 beta-triol or cholesterol-5 alpha, 6 beta-epoxide. Endocytosis, as measured by uptake of horseradish peroxidase (HRP), was inhibited in a dose and time dependent manner in both endothelial and smooth muscle cell cultures by cholestane-3 beta,5 alpha,6 beta-triol and 25-hydroxycholesterol. Inhibition by 7-ketocholesterol in endothelial cells occurred only at higher concentrations, and cholesterol and cholesterol epoxide showed no significant inhibitory effects. The viability of the cells exposed to the cholesterol oxides at the concentrations that inhibited the uptake of HRP was not changed. Cholesterol oxides induce functional endothelial injury, not morphologically apparent, which may be involved in atherogenesis.
Defective insulin secretion in pancreatic β cells lacking type 1 IGF receptor (2002)

Defective insulin secretion is a feature of type 2 diabetes that results from inadequate compensatory increase of beta cell mass and impaired glucose-dependent insulin release. beta cell proliferation and secretion are thought to be regulated by signaling through receptor tyrosine kinases. In this regard, we sought to examine the potential proliferative and/or antiapoptotic role of IGFs in beta cells by tissue-specific conditional mutagenesis ablating type 1 IGF receptor (IGF1R) signaling. Unexpectedly, lack of functional IGF1R did not affect beta cell mass, but resulted in age-dependent impairment of glucose tolerance, associated with a decrease of glucose- and arginine-dependent insulin release. These observations reveal a requirement of IGF1R-mediated signaling for insulin secretion.
ChemInform Abstract: APPLICATION OF REACTIONS OF β‐KETO SULFOXIDES TO ESTRONE SYNTHESIS (1976)

Abstract Durch thermische Eliminierung der Sulfoxidreste der β‐Ketosulfoxide (II), die aus den Estern (I) und Sulfoxiden erhältlich sind, entstehen in Gegenwart von Methylcyclopentandion das Trion (III) (Ausbeute bis 70 %) und das Dion (IV) (Ausbeute bis 35 %).
Mapping nested loop algorithms into multidimensional systolic arrays (1990)

Consideration is given to transforming depth p-nested for loop algorithms into q-dimensional systolic VLSI arrays where 1<or=q<or=p-1. Previously, there existed complete characterizations of correct transformation only for the cases where q=p-1 or q=1. This gap is filled by giving formal necessary and sufficient conditions for correct transformation of a p-nested loop algorithm into a q-dimensional systolic array for any q, 1<or=q<or=p-1. Practical methods are presented. The techniques developed are applied to the automatic design of special purpose and programmable systolic arrays. The results also contribute toward automatic compilation onto more general purpose programmable arrays. Synthesis of linear and planar systolic array implementations for a three-dimensional cube-graph algorithm and a reindexed Warshall-Floyd path-finding algorithm are used to illustrate the method.<<ETX>>
Microbial lipases: production and applications. (1996)

Lipases occupy a prominent place among biocatalysts and have a wide spectrum of biotechnological applications. Lipases are unique as they hydrolyse fats into fatty acids and glycerol at the water-lipid interface and can reverse the reaction in non-aqueous media. The stability of these enzymes in organic solvents have pushed them into the frontier areas of organic synthesis leading to the designing of novel drugs, surfactants, bioactive compounds and oleochemicals. In addition, lipase-catalysed trans-esterification and inter-esterification reactions have been exploited in the fat industry. Looking into the wide scenario of lipase applications, commercialization of lipase production is a prime area of interest for microbiologists, process engineers and biochemists. Research carried out in this field has revealed that microbes, especially fungi and bacteria, are the tools of choice for commercial production. Recently, the structure determination of a few microbial lipases has widened our knowledge about the unique mechanism of catalysis of this enzyme.
Microwave-Assisted Cu-Catalyzed Protodecarboxylation of Aromatic Carboxylic Acids (2009)

An effective protocol has been developed that allows the smooth protodecarboxylation of diversely functionalized aromatic carboxylic acids within 5-15 min. In the presence of at most 5 mol % of an inexpensive catalyst generated in situ from copper(I) oxide and 1,10-phenanthroline, even nonactivated benzoates were converted in high yields and with great preparative ease.
<i>Helicobacter pylori</i> -Induced Mucosal Inflammation Is Th1 Mediated and Exacerbated in IL-4, But Not IFN-γ, Gene-Deficient Mice (2000)

To elucidate the pathogenesis of Helicobacter pylori-associated gastritis, we studied immune responses of C57BL/6J wild-type (WT), SCID, and gene deficient (IFN-gamma-/- and IL-4-/-) mice following infection with a pathogenic isolate of H. pylori (SPM326). During early infection in WT mice, mononuclear and polymorphonuclear cells accumulated in the gastric lamina propria, and the numbers of cells in the inflamed mucosa expressing IFN-gamma, but not IL-4, mRNA rose significantly (p < 0.005), consistent with a local Th1 response. Splenic T cells from the same infected WT mice produced high levels of IFN-gamma, no detectable IL-4, and low amounts of IL-10 following in vitro H. pylori urease stimulation, reflecting a systemic Th1 response. Infected C57BL/6J SCID mice did not develop gastric inflammation despite colonization by many bacteria. Infected C57BL/10J and BALB/c mice also did not develop gastric inflammation and displayed a mixed Th1/Th2 splenic cytokine profile. These data imply a major role for the Th1 cytokine IFN-gamma in H. pylori-associated gastric inflammation in C57BL/6J mice. Compared with WT animals, infected IL-4-/- animals had more severe gastritis and higher levels of IFN-gamma production by urease-stimulated splenocytes (p < 0.01), whereas IFN-gamma-/- mice exhibited no gastric inflammation and higher levels of IL-4 production by stimulated splenocytes. These findings establish C57BL/6J mice as an important model for H. pylori infection and demonstrate that up-regulated production of IFN-gamma, in the absence of the opposing effects of IL-4 (and possibly IL-10), plays a pivotal role in promoting H. pylori-induced mucosal inflammation.
Experimental Validation of Fatigue Life of CCGA 624 Package with Initial Contact Pressure of Thermal Gap Pads under Random Vibration Excitation (2018)

Thermal gap pads are widely used for effective heat transfer from high-heat-dissipating components to a heat sink. The initial contact pressure of thermal pads is an important parameter for enhancing the heat transfer capability of thermal design during the assembly process of the heat sink. However, this causes stress on the solder joint of the component as a result of the pad’s inherent resistance to deformation. In this study, we investigated the effect of thermal pad on the fatigue life of the solder joint of CCGA 624 package for space usage. A random vibration fatigue test for several specimen assembly sets with various pressurization levels of thermal gap pads was performed, and their fatigue lives were compared with that of the package without the initial pressure of thermal gap pads.
Pigskin as a topical hemostat in arterial, liver, and splenic injuries. (1986)

Pigskin made commercially available as porcine-derived dermal collagen (PDC) contains a macrofibrillar collagen surface that initiates platelet aggregation and the intrinsic pathway clotting mechanism. Sterile-irradiated PDC, nonirradiated PDC, and lyophilized PDC were tested for hemostatic effectiveness and cost for hemostasis against each other and Avitene powder, Avitene web, Collastat, Gelfoam, Superstat, and Thrombostat. Canine arterial, liver, and splenic bleeding models were used. In arterial wounds PDC was more effective than Avitene powder, Avitene web, and Gelfoam (p less than 0.05) and also Superstat and Thrombostat (p less than 0.01). First application success rates revealed that PDC agents were more successful overall (85%) than the next three most successful agents: Avitene powder, Avitene web, and Collastat (56%) (p less than 0.01). Differences in success rates of hepatic and splenic wounds were less marked but still significant. Cost of hemostasis was relatively low for PDC agents in comparison to the other agents. PDC is an effective topical hemostatic agent in experimental canine arterial, liver, and splenic wounds when compared with several currently used agents and can also result in cost savings.
The E-Cadherin expression and its clinical significance of lymph node micrometastases in colorectal carcinoma (2010)

Objective Purpose To detect the expression of E-cadherin in colorectal carcinoma and lymph node micrometastases,and investigate their relationship.Methods 82 cases of lymph node simple was dectected with RT-PCR method using CK20 mRNA acridinium ester.in screening colorectal carcinoma lymph node micrometastases.E-Cadherin expression analysed by immuno-histochemical staining(the SP method) in colorectal cancer tissue,tissue nearby cancer,the normal control tissue.Results In 60 cases of colorectal carcinoma,the expression of E-Cadherin was 71.67%.Number of positive nodes was 55.8%,the expression of E-cadherin was 56.7%(34 cases) and number of positive nodes was 45.5%(356 cases) through routine pathological examination.The low level expression of E-Cadherin was positively correlated with the Dukes staging,serosa infiltration,lymph node and liver metastasis of colorectal carcinoma(x2 = 16.72,P ＜ 0.05).The expression of E-Cadherin was negative in 6 of the 11 patients with lymph node micrometastases,and in 6.67% 1 of the 15 patients without lymph node micrometastases(x2 = 4.160,P = 0.041).Conclusion The negative expression of E-Cadherin may contribute to lymph node micrometastases in colorectal carcinoma.

Key words: 
colorectal carcinoma; E-Cadherin; Lymphatic metastasis; Rt-pcr
Remote Denial of Service Attacks and Countermeasures (2001)

In November 2002, Chief Judge Judith Kaye attended the National Adoption Day festivities in Albany County and New York County (Manhattan). Although pleased that 600 adoptions were being finalized statewide on this special day, she was concerned to learn more than 6,000 other children were free for adoption but had not yet found permanent families. Judge Kaye reached out to New York State Office of Children and Family Services (OCFS) Commissioner John A. Johnson and New York City Administration for Children's Services (ACS) then-Commissioner William Bell to come together and begin a discussion to identify and resolve systemic barriers to adoption. At a press conference in May 2003, Chief Judge Kaye, Governor Pataki, New York City Mayor Bloomberg, and Judges and Commissioners representing counties from across the state announced the Adoption Now initiative that set a goal of finalizing adoptions for 5,000 children (3800 in New York City and 1200 upstate) by the end of 2003.