gebhart/patrepeval huggingface dataset:
Hormone Interactions to Leu-rich Repeats in the Gonadotropin Receptors (2001)

The luteinizing hormone receptor (LHR) consists of an approximately 350-amino acid-long N-terminal extracellular exodomain and a membrane-associated endodomain of similar size. Human chorionic gonadotropin (hCG) binds to the exodomain, and then hCG/exodomain complex is thought to make a secondary contact with the endodomain and generate hormone signals. The sequence alignment of the exodomain shows imperfectly matching eight to nine Leu-rich repeats (LRRs). In the preceding article (Song, Y., Ji, I., Beauchamp, J., Isaacs, N., and Ji, T. (2001) J. Biol. Chem. 276, 3426-3435), we have shown that LRR2 and LRR4 are crucial for hormone binding. In this work, we have examined the residues of LRR4, in particular Leu(103) and Ile(105) in the putative beta strand. Our data show that Leu(103) and Ile(105) are involved in the specific, hydrophobic interaction of the LRR4 loop, likely to form the hydrophobic core. This loop is crucial for the structural integrity of all of the LRRs. In contrast, the downstream sequence consisting of Asn(107), Thr(108), Gly(109), and Ile(110) of LRR4 is crucial for cAMP induction but not for hormone binding, folding, and surface expression. This implicates, for the first time, its involvement in the interaction with the endodomain and signal generation. The evidence for the interaction is presented in the following article.
CT ESP for Yme, Converting the Yme Field Offshore Norway from a Conventional Rig-Operated Field to CT-Operated for Workover and Drilling Applications (1998)

Abstract Conventional tubing deployed ESP was initially chosen as the artificial lift method for the Yme field offshore Norway. Two years of operational experience proved that the method chosen did not meet the original design criteria with regards to preventing fluid loss to the formation during workovers and access to the reservoir with wireline/coiled tubing. In addition, the average ESP lifetime was initially well below the target runlife. The current ESP-completion design is described. Main operational problems experienced during two years of operation is highlighted. The revised field requirements and a re-evaluation of artificial lift methods proved the need for a change in completion philosophy. Coiled Tubing Deployed ESPs (CT-ESP) utilizing an internally installed power cable became the preferred method. The value of installing CT-ESP in a live well is discussed. System design for true underbalanced CT-ESP installation is described. Well control and barrier philosophy during installation, production and workover mode is also assessed.
Effects of a Microbial Inoculant on Fermentation Characteristics and Nutritional Value of Corn Silage (1989)

A study was conducted to evaluate the effect of a microbial inoculant on whole plant corn silage fermentation characteristics and nutritive value. Microbial inoculant was added to the corn silage at the rate of 200 000 colony forming units (CFU)/g of wet crop. The epiphytic population of lactic acid‐producing microorganisms was 4 million CFU/g. Silages were stored in bunker silos for 151 d prior to feeding. Collection of effluent and measurements of temperature and pH were done for 72 d post‐harvest. Ninety‐six Hereford × Angus ( Bos taunts ) crossbred steers were used to evaluate nutritional value of the fermented silages. Rate of deterioration and temperature changes upon exposure to oxygen were monitored to evaluate bunk stability. Treated silage had a lower temperature between 25–90 d post‐ensiling ( P < 0.01) than the control silage. During the feedout period, lactic acid levels were greater ( P < 0.01) for the inoculated silage. No differences in numbers of Clostridia, lactobacilli, yeast, and molds were found between treatments. The increase in lactic acid production and lower temperature without increased numbers of lactic acid producing microorganisms for the inoculated silage suggests a shift in the fermentation pattern occurred. The inoculated silage upon exposure to air deteriorated at a faster rate than the control silage. Animal weight gains and dry matter intakes were similar for both silage treatments. The results of this study suggest inoculating corn silage with microbial inoculant did not affect its nutritive value but appeared to lower the stability of corn silage to air exposure.
Spiropyran-doped poly(vinyl carbazole): a new photopolymer recording medium for erasable holography (1995)

The photosensitive organic system of poly(vinyl carbazole) doped with spiropyran is studied for real-time reversible holography. The photoinduced reversible color change between thermally stable and metastable states of spiropyran molecules can modulate the absorption and the refractive index of the spiropyran-doped polymer film. Erasable holograms can be recorded in either stable or metastable states of such films of thickness 10 and 50 μm prepared by a gravity settling method. The grating constant achieved was δ= 0.58 μm, using the 350-nm UV line of a krypton-ion laser. It has been found that write-read-erase cycles can be performed repeatedly. High-efficiency erasable holographic recording at 350 nm has been performed in these films, and a diffraction efficiency greater than 10% has been achieved.
Magnetic Recording Heads (1999)

Abstract The sections in this article are Basic Operation Types of Recording Heads Head Materials and Processing Theory of Operation Recording Head Testing Future Directions
Decision Lists for Lexical Ambiguity Resolution: Application to Accent Restoration in Spanish and French (1994)

This paper presents a statistical decision procedure for lexical ambiguity resolution. The algorithm exploits both local syntactic patterns and more distant collocational evidence, generating an efficient, effective, and highly perspicuous recipe for resolving a given ambiguity. By identifying and utilizing only the single best disambiguating evidence in a target context, the algorithm avoids the problematic complex modeling of statistical dependencies. Although directly applicable to a wide class of ambiguities, the algorithm is described and evaluated in a realistic case study, the problem of restoring missing accents in Spanish and French text.
Synthetic studies directed toward cembranolides. Synthesis of the basic nucleus of crassin acetate (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic studies directed toward cembranolides. Synthesis of the basic nucleus of crassin acetateWilliam G. Dauben, R. Kent Saugier, and Ilona FleischhauerCite this: J. Org. Chem. 1985, 50, 20, 3767–3774Publication Date (Print):October 1, 1985Publication History Published online1 May 2002Published inissue 1 October 1985https://pubs.acs.org/doi/10.1021/jo00220a018https://doi.org/10.1021/jo00220a018research-articleACS PublicationsRequest reuse permissionsArticle Views555Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens (1989)

Microbiology Society journals contain high-quality research papers and topical review articles. We are a not-for-profit publisher and we support and invest in the microbiology community, to the benefit of everyone. This supports our principal goal to develop, expand and strengthen the networks available to our members so that they can generate new knowledge about microbes and ensure that it is shared with other communities.
Variable Microelectronic Inductors (1978)

A thin-film microelectronic inductor is proposed. It is suitable for use in the frequency range from 5 to 20 MHz. The inductor is in the shape of a square spiral and is surrounded by patterned permalloy thin films. The pattern allows these films to be driven in their easy axes of magnetization by the high-frequency signal current. In the presence of a small dc magnetic field applied in the hard direction and equal to the anisotropy field, the resulting permeability is very high, and thus the inductance of the pattern is greatly increased. Since the inductance is a function of the dc magnetic field applied in the hard direction, variable inductors and tunable planar resonant circuits are readily implemented using the proposed device. The design considerations of the device are described, and the significance of the theoretical results is discussed. Experimental work is being carried out to verify the above results.
A Neural Circuit for Memory Specificity and Generalization (2013)

Increased fear memory generalization is associated with posttraumatic stress disorder, but the circuit mechanisms that regulate memory specificity remain unclear. Here, we define a neural circuit-composed of the medial prefrontal cortex, the nucleus reuniens (NR), and the hippocampus-that controls fear memory generalization. Inactivation of prefrontal inputs into the NR or direct silencing of NR projections enhanced fear memory generalization, whereas constitutive activation of NR neurons decreased memory generalization. Direct optogenetic activation of phasic and tonic action-potential firing of NR neurons during memory acquisition enhanced or reduced memory generalization, respectively. We propose that the NR determines the specificity and generalization of memory attributes for a particular context by processing information from the medial prefrontal cortex en route to the hippocampus.
A portable flow-through fluorescent immunoassay lab-on-a-chip device using ZnO nanorod-decorated glass capillaries (2013)

We report a portable flow-through fluorescent immunoassay lab-on-a-chip device using inexpensive disposable glass capillaries for medical diagnostics. The device is made up of a number of serially connected glass capillaries, of which each interior surface is grown using zinc oxide (ZnO) nanorods, which different probe antibodies are attached to. The ZnO nanorods not only provide a large surface area for high density probe attachment, but also enhance the fluorescent signals to significantly improve the detection signal responses. The glass capillary also allows for convenient flow-through detection. Coupled with a homemade handheld analyzer integrated with an automatic pump system and a fluorescence readout module, a portable immunoassay capillary device enables quantitative detection of multiple biomarkers in 30 min with detection limits of 1-5 ng mL(-1) and wide dynamic ranges for prostate specific antigen (PSA), α-Fetoprotein (AFP) and carcinoembryonic antigen (CEA) in serum. This new conceptual lab-on-a-chip device eliminates the need for expensive micro-fabrication processes, while offering inexpensive and disposable, but replaceable tube-type "microchannels" for multiplexed detection in portable clinical diagnostics.
Quantitative force versus distance measurements in amplitude modulation AFM: a novel force inversion technique (2009)

A new method for extracting quantitative data from amplitude modulation dynamic force-distance measurements is developed. The method is based on the harmonic oscillator model of vibrating atomic force microscope cantilevers, and is capable of extracting both the conservative and dissipative parts of the tip-sample interaction from a measurement of oscillation amplitude and phase as a function of distance. Numerical simulations are used to demonstrate the validity of the method. Further proof of the accuracy of this method is provided by a measurement of electrostatic forces between an AFM tip and a graphite sample.
Identification of a thyroid hormone response element in the malic enzyme gene. (1990)

We have studied the rat malic enzyme gene as a model for thyroid hormone (triiodothyronine (T3)) regulation of transcription. Our previous studies showed that transcription of this gene is controlled by T3 in vivo. In this study, we used COS-7 cells in culture to determine the location of a T3 response element (TRE) within this gene. Cotransfection of the rat T3 alpha-receptor cDNA with a reporter gene linked to 5'-deletion mutants of the malic enzyme gene 5'-flanking region revealed the presence of a TRE between positions -315 and -248. Using T3 receptors synthesized in vitro from their cDNAs, we have identified, through the use of gel shift assays and footprinting, a single DNA-binding site (positions -281 to -261) for the receptor within the rat malic enzyme gene TRE. The site was capable of binding either the rat alpha- or human beta-receptor with similar affinities. Competition binding studies indicated that the apparent affinity of receptor binding to the malic enzyme gene was similar to that of the rat alpha-myosin heavy chain gene TRE (positions -151 to -122) and was significantly greater than that of the rat growth hormone TRE (positions -192 to -163). These results indicate that both the alpha- and beta-forms of the nuclear T3 receptor are capable of binding directly to the malic enzyme gene 5'-flanking region at a site which functions as a hormone-inducible cis-regulatory element. In conjunction with our previous finding that T3 induces or activates essential transcription factors which bind to the promoter, it appears that the regulation of transcription of the malic enzyme gene by thyroid hormone involves at least two regulatory pathways.
A MOF-derived coral-like NiSe@NC nanohybrid: an efficient electrocatalyst for the hydrogen evolution reaction at all pH values (2018)

A coral-like NiSe@NC nanohybrid as an effective electrocatalyst for the hydrogen evolution reaction (HER) at all pH values, constructed via the in situ selenation of a Ni-MOFs precursor, is reported. The electrocatalyst shows overpotentials of 123 mV, 250 mV and 300 mV in 0.5 M H2SO4, 1.0 M KOH and 1.0 M PBS, respectively, to afford a current density of 10 mA cm-2. Meanwhile, NiSe@NC also exhibits a small Tafel slope and superior long-term stability over a wide pH range. The excellent electrocatalytic performance should be ascribed to the unique coral-like structure with a large BET specific surface area (125.4 m2 g-1) and mesoporous features, as well as synergistic effects between NiSe nanocrystals and highly conductive N-doped porous carbon.
Effects Of Digital Filtering On The Waveform & Peak Parameters Of The Auditory Brainstem Response (1983)

Since the auditory brainstem response (ABR) has a narrower bandwidth than the spontaneous EEG, filtering can be used to improve the signal-to-noise ratio (SNR) prior to averaging. The purpose of this study is to compare the effects of different digital filters. Filters evaluated included lowpass (40–1600 Hz), bandpass (200–1600 Hz), and time invariant a-posterioriWiener filters. All filters were digitally implemented and had zero phase-shift. The filters were applied to averaged auditory brainstem responses based on different numbers of individual responses (128 to 1,024). The effects of the filters on both root-mean-square (rms) error and on Jewett wave parameters (peak latency and amplitude) were considered. The lowpass filter did not affect the rms error or wave amplitude; it did reduce the wave latency variability, but it did not affect the mean latency value. It greatly facilitated wave identification by removing high frequency noise. The bandpass filter behaved similarly, except that a slow wave activity in the response was removed also, resulting in an increased rms error. The performance of the Wiener filters was essentially the same as the lowpass filters except for a tendency to attenuate the waveform. In evaluating these filtering techniques, both waveform mean square error and wave latency and amplitude variability should be considered.
In vitro antimicrobial activity of Luffa operculata (2014)

L. operculata extract showed promising antibacterial activity in vitro against the studied agents.
Direct HPLC Analysis of Quercetin and <i>trans</i>-Resveratrol in Red Wine, Grape, and Winemaking Byproducts (2003)

A simple and fast reversed-phase HPLC method using diode array detection was developed and validated for the simultaneous determination of trans-resveratrol and quercetin in Sicilian red wine from the Nero d'Avola red grape variety. Investigation was also extended to the quantitative determination of resveratrol and quercetin in grape skins and winemaking byproducts obtained from the same cultivar. Samples were eluted using a C18 narrow-bore column under isocratic conditions in less than 20 min. Quantification of trans-resveratrol and quercetin in red wine was performed without any sample pretreatment, whereas the determination of these phenolic compounds in grape skins and wine pomage required a solvent extraction procedure. Linearity was demonstrated over the 0.39-12.5 and 0.45-57.6 microg/mL range for trans-resveratrol and quercetin, respectively. Detection limits in real samples were in the low ppm level (0.07 mg/L for trans-resveratrol and 0.12 mg/L for quercetin). The HPLC-UV/DAD method was applied for the routine analyses of red wine and grape skin and winemaking byproduct extracts to evaluate their trans-resveratrol and quercetin content. In particular, a very high content of quercetin was found in wine pomace, suggesting the use of this wine byproduct as a potential source of this health-promoting phenolic compound.
Potentiation of a sodium–calcium exchanger in the nuclear envelope by nuclear GM1 ganglioside (2002)

Calcium is recognized as an important intracellular messenger with a pivotal role in the regulation of many cytosolic and nuclear processes. Gangliosides of various types, especially GM1, are known to have a role in some aspects of Ca2+ regulation, operating through a variety of mechanisms that are gradually coming to light. The present study provides evidence for a sodium-calcium exchanger in the nuclear envelope of NG108-15 neuroblastoma cells that is potently and specifically activated by GM1. Immunoblot analysis revealed an unusually tight association of GM1 with the exchanger in the nuclear envelope but not with that in the plasma membrane. Exchanger and associated GM1 were located in the inner membrane of the nuclear envelope, suggesting this system could function to transfer Ca2+ between nucleoplasm and the envelope lumen. The GM1-enhanced exchange was blocked by cholera toxin B subunit while C2-ceramide, a recently discovered inhibitor of the exchanger, blocked all transfer. Exchanger activity was significantly elevated in nuclei isolated from cells that were induced to differentiate by KCl + dibutyryl-cAMP, a treatment previously shown to promote up-regulation of nuclear GM1 in conjunction with axonogenesis. Similar enhancement was achieved by addition of exogenous GM1 to nuclei from undifferentiated cells. These results suggest a prominent role for nuclear GM1 in regulation of nuclear Ca2+ homeostasis.
Quantitative Evaluation of Carotid Plaque Composition by In Vivo MRI (2004)

MRI-based tissue quantification is accurate and reproducible. This application can be used in therapeutic clinical trials and in prospective longitudinal studies to examine carotid atherosclerotic plaque progression and regression.
Genetics and Utilization of Pathogen Resistance in Plants (1996)

Ian R. Crute, David A. C. Pink, Genetics and Utilization of Pathogen Resistance in Plants, The Plant Cell, Vol. 8, No. 10, Special Review Issue on Plant-Microbe Interactions (Oct., 1996), pp. 1747-1755
Bradykinin Antagonists: The State of the Art (1992)

The availability of practical bradykinin antagonists has revolutionized research in the kinin field. Development of the first antagonists and progress to the present time are reviewed.
Thermally isolated microelectronic devices for gas sensing applications. (2001)

Note: Jury: M. Koudelka-Hep (IMT-UniNE), I. Lunstrom (Linkoping Uni, Sweden), B. van der Schoot (Seyonic, Neuchâtel) Reference SAMLAB-THESIS-2001-002 Record created on 2009-05-12, modified on 2016-08-08
Ultrasound-guided botulinum toxin injection technique for the iliopsoas muscle (2003)

Intramuscular botulinum toxin A injections are beneficial for the treatment of functional shortening of the iliopsoas muscle, but it is difficult to achieve precise needle positioning and injection. As a solution to this we present an ultrasound-guided injection technique for the iliopsoas muscle using an anterior approach from the groin. The procedure was performed 26 times in 13 patients (seven males, six females; mean age 11 years, SD 9 years 8 months; age range 4 to 31 years), 10 times bilaterally. Indications were functional iliopsoas shortening due to cerebral palsy (17 hips), hereditary spastic paraplegia (four hips), and Perthes disease (five hips). In all cases the iliopsoas muscle was identified easily by ultrasound; the placement of the injection needle and injection into the site of interest were observed during real time. No complications were encountered. Botulinum toxin A (BTX-A) injections have become established as a standard procedure for the treatment of functional shortening of different muscles in persons with spasticity or dystonia (Kessler et al. 1999, Bakheit et al. 2001, Kirschner et al. 2001). Optimal needle placement is essential to avoid severe side effects and to assess lack of response to the drug or incorrect region of injection. While injection into superficial, very palpable muscles is quite easy, the approach to other muscles such as the iliopsoas muscle may be more difficult and the placement of the needle for an optimal injection site is harder to control. As a solution to this, we present an ultrasound-guided injection technique. The main indications for BTX-A injections in the iliopsoas muscle are dynamic hip flexion deformities mostly due to spastic conditions which may compromise walking (increased anterior pelvic tilt during the whole gait cycle, decreased hip extension at terminal stance, increased peak hip flexion during swing; Molenaers et al. 1999. Another indication might be decentration of the femoral head (as part of an injection programme which also includes other muscles like the adductors and the medial hamstrings) for pain relief, reducing care difficulties and, possibly, prevention of further decentration (Porta 2000, Foster et al. 2001, Deleplanque et al. 2002, Lubik et al. 2002). In Perthes disease, BTX-A injections in the iliopsoas muscle and the adductors may prevent a fixed deformity, which is a negative prognostic factor.