gebhart/patrepeval huggingface dataset:
Sequestration of Trace Metals Using Water-Soluble and Fluorous Phase-Soluble Polymers (2000)

Ion-exchange polymers with “on”/“off” solubility can be prepared from N-isopropylacrylamide (PNIPAM) copolymers that incorporate hydroxamic acid ligands. Such complexing agents quantitatively remove trace amounts of FeIII from aqueous solutions (vial 1) by precipitating the metal – polymer complex on heating (suspension in vial 2). Physical separation of the insoluble polymer leaves a metal-free solution (vial 3). Similar fluorous phase-soluble hydroxamic acid derivatives of fluoroacrylate polymers remove FeIII ions from organic solutions by liquid/liquid phase separation.
A new modeling and optimization of gain-boosted cascode amplifier for high-speed and low-voltage applications (2006)

Because of limited output swing, the basic structure of a gain-boosted cascode amplifier (GBCA) cannot be used to design a low-voltage operational amplifier. In this paper, we investigate the design of a high-swing GBCA, and find the optimum bandwidth for the boosting or feedback amplifier. This bandwidth eliminates the slow-settling component in the step response, prevents the gain-boosting loop from being unstable, and results in the shortest settling time. Finally, we present a very high-speed and high-swing amplifier suitable for low-voltage applications.
Effect of training and nutrition on the development of skeletal muscle (1995)

Recent data suggest that an increased muscle mitochondrial oxidative capacity is not a prerequisite for a marked metabolic effect of endurance training, but instead may represent an adaptive phenomenon to the new metabolic situation. The muscle content of the glucose transporter GLUT-4 increases after one or only a few exercise sessions. The ensuring enhancement of the insulin sensitivity allows faster replenishment of muscle glycogen stores following exercise bouts. There is evidence that the muscle content of GLUT-4 declines more slowly with inactivity than the muscle oxidative capacity. This may be a sign that, as in the chronically stimulated rabbit muscle, adaptive changes in human skeletal muscle follow a "first-in, last-out' sequence. There is evidence that muscle (and possibly also plasma) triacylglycerol is more important as an energy source during exercise than was previously recognized. Endurance training increases the content of slow isoforms of myosin in the muscle, and information on changes in muscle shortening velocity and on the molecular regulation of muscle volume is emerging. beta 2-agonists are well documented to enhance muscle mass, whereas creatine supplementation appears to enhance performance during high-intensity exercise.
Predictive value of annual costs on 3-year mortality in COPD (2015)

Clinical signs and lung function are variably sensitive in predicting death in COPD phenotypes. <b>Aim:</b> To assess predictive value of annual COPD costs on 3-yr mortality. <b>Methods:</b> Gender, age, complete lung function and clinical data of 275 consecutive COPD patients (aged >40 yrs) were assessed at recruitment (T<sub>0</sub>) and annual costs calculated over preceding 12 mths. <b>Statistics:</b> t tests to compare means ±SD; regression analysis to determine relationship between variables and survival (days) (p<0.05). <b>Results:</b> Mortality: 40.4% over 3 yrs (n=12; 47 and 52 subjects deceased). At T<sub>0</sub>, survivors (n=164 after 3 yrs) were significantly different from those (n=111) who deceased over 3 yrs by mean age, FEV1 (in L), RV, DLCO/VA, 69 wt, BODE index, and Charlson index (all p<0.001), but not by FEV1% pred. or FEV1/FVC (p=ns). At T<sub>0</sub>, mean total cost was €3,290.7[95%CI: 2,539.9; 4,051.2] and €1,161.0 [95% CI: 968.4; 1,353.6] in survivors and €6,158.9 [95% CI: 5,508.0; 6,809.8] in deceased (p<0.001). Hospitalization cost was 78.2% of total annual cost in deceased subjects, and their absolute value was 6-fold higher than in survivors. Regression between cost and survival showed the highest significance (p<0.0001; r=0.60). All cost components were also discriminant, independently of gender. <b>Conclusions:</b> 1) Clinical and lung function variables contribute to predicting mortality in COPD. 2)Total annual cost was the most sensitive predictor of 3-yr mortality. 3) Present data support the high predictive value of 9economic phenotyping9 in COPD.
DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis (2012)

Cyclic dinucleotides, including cyclic di-AMP (c-di-AMP), are known to be ubiquitous second messengers involved in bacterial signal transduction. However, no transcriptional regulator has been characterized as a c-di-AMP receptor/effector to date. In the present study, using a c-di-AMP/transcription factor binding screen, we identified Ms5346, a TetR family regulator in Mycobacterium smegmatis, as a c-di-AMP receptor in bacteria. Ms5346 could specifically bind c-di-AMP with K(d) of 2.3 ± 0.5 μM. Using EMSA and DNase I footprinting assays, c-di-AMP was found to stimulate the DNA binding activity of Ms5346 and to enhance its ability to protect its target DNA sequence. A conserved 14-bp palindromic motif was identified as the DNA-binding site for Ms5346. Further, Ms5346 was found to negatively regulate expression of three target genes including Ms5347 (encoding a major facilitator family transporter), Ms5348 (encoding a medium chain fatty acyl-CoA ligase), and Ms5696 (encoding a cold shock protein, CspA). Ms5346 is the first cyclic di-AMP receptor regulator to be identified in bacteria, and we have designated it as DarR. Our findings enhance our understanding of the function and regulatory mechanism of the second messenger c-di-AMP in bacteria.
Human and mouse LSP1 genes code for highly conserved phosphoproteins. (1990)

With use of the mouse LSP1 cDNA we isolated a human homologue of the mouse LSP1 gene from a human CTL cDNA library. The predicted protein sequence of human LSP1 is compared with the predicted mouse LSP1 protein sequence and regions of homology are identified in order to predict structural features of the LSP1 protein that might be important for its function. Both the human and mouse LSP1 proteins consist of two domains, an N-terminal acidic domain and a C-terminal basic domain. The C-terminal domains of the mouse and human LSP1 proteins are highly conserved and include several conserved, putative serine/threonine phosphorylation sites. Immunoprecipitation of LSP1 protein from 32P-orthophosphate-loaded cells show that both the mouse and human LSP1 proteins are phosphoproteins. The sequences of the putative Ca2(+)-binding sites present in the N-terminal domain of the mouse LSP1 protein are not conserved in the human LSP1 protein; however, a different Ca2(+)-binding site may exist in the human protein, indicating a functional conservation rather than a strict sequence conservation of the two proteins. The expression of the human LSP1 gene follows the same pattern as the expression of the mouse LSP1 gene. Southern analysis of human genomic DNA shows multiple LSP1-related fragments of varying intensity in contrast to the simple pattern found after similar analysis of mouse genomic DNA. By using different parts of the human LSP1 cDNA as a probe, we show that most of these multiple bands contain sequences homologous to the conserved C-terminal region of the LSP1 cDNA. This suggests that there are several LSP1-related genes present in the human genome.
Synthesis and Photophysical Properties of Pyrene-Based Multiply Conjugated Shaped Light-Emitting Architectures: Toward Efficient Organic-Light-Emitting Diodes (2011)

Since the pioneering works on the first double-layer thin-film Organic electroluminescence (EL) devices (OLEDs) by C.
Application of Hydrogen Peroxide as an Innovative Method of Treatment for Legionella Control in a Hospital Water Network (2017)

Hydrogen peroxide demonstrated good efficacy in controlling <i>Legionella</i>. Although in the initial phases of treatment it appeared unable to eliminate all <i>Legionella</i> species, by maintaining HP levels at 25 mg/L and adding food-grade polyphosphates, a progressive and complete control of colonization was obtained.
전도성 고분자 패턴을 이용한 전기변색 회절 편광자 (2011)

Polydimethylsiloxane(PDMS) 몰드를 사용하여 소프트 리소그래피 방법을 통해 Poly(3-hexylthiophene) (P3HT)가 서브파장(subwavelength) 사이즈로 나노 패턴된 전기변색소자를 제작하였다. 나노 패턴된 전기변색소자를 사용하여 착색 및 소색 상태에 따른 빛의 편광효과를 패턴의 방향을 바꿔가며 측정하였으며, 인가전압에 따라 변화되는 고분자의 도핑 상태에 의해서 편광된 빛의 세기를 손쉽게 가역적으로 변화시킬 수 있었다. 편광 효율을 최대값과 최소값의 비로 정의하여 실험을 통해 1.7의 편광 효율값을 계산할 수 있었다. 산화, 환원 반응에 따른 고분자의 구조변화에 의해 도핑 상태에 따라 굴절률 차이가 생기게 되고 이에 따라 편광된 빛의 세기를 인가전압의 스위칭만으로 조절할 수 있었다. 전기변색소자에 -2V와 2V 전압을 인가해 주었을 때 고분자 패턴과 전해질 사이의 굴절륯 차이는 0.61과 0.99의 값으로 나타났다.
Structural and functional analysis of PTPMT1, a phosphatase required for cardiolipin synthesis (2011)

PTPMT1 (PTP localized to the Mitochondrion 1) is a member of the protein tyrosine phosphatase superfamily that is localized exclusively to the mitochondrion. We recently reported that PTPMT1 dephosphorylates phosphatidylglycerol phosphate, an essential intermediate of cardiolipin biosynthesis. To gain further insights into the molecular basis of PTPMT1 function, we determined the crystal structures of the phosphatase domain of PTPMT1. PTPMT1 exhibits a canonical protein tyrosine phosphatase domain fold, resembling many dual-specificity phosphatases such as phosphatase and tensin homolog and vaccinia H1-related phosphatase. We also determined the structure of the catalytically inactive phosphatase in complex with a surrogate substrate, phosphatidylinositol 5-phosphate, which sheds light on the substrate recognition and specificity of PTPMT1. Comparison of the apo and substrate-bound structures of PTPMT1 suggests that it undergoes significant conformational change during catalysis, and we further demonstrated that an evolutionarily conserved EEYE loop is important for its activity.
Über Diacetyl‐indigo (1919)

Mitteilung aus dem Chemiechen Inetitut der Univeraitfit Halle.] (Eingegangen am 1.
Solvent‐free synthesis of polyols from 1‐butene metathesized palm oil for use in polyurethane foams (2016)

ABSTRACT Green Polyols were synthesized from a 1‐butene cross metathesized palm oil (PMTAG) using a green, solvent free epoxidation and hydroxylation pathway. The synthetic strategy was adapted to control the degree of double bond epoxidation and ultimately the hydroxyl value of the polyols. The polyols comprised diol and tetrol monomers with terminal hydroxyl groups content as high as ∼18 mol %, and achieved hydroxyl values between 83 and 119 mg KOH g −1 . Functional Rigid and highly flexible foams were prepared from two designer Green Polyols. The foams presented a high thermal stability ( T on of degradation of ∼270 °C), suitable glass transition temperatures (∼−12 °C and ∼50 °C) and compressive strength (0.21 MPa at 10% strain and ∼1 MPa at 10% strain for the flexible and rigid foams, respectively) which are superior to existing lipid‐based counterparts. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 43509.
Chemoattractant properties of PR-39, a neutrophil antibacterial peptide (1997)

The proline-arginine (PR) -rich antibacterial peptide, PR-39, kills bacteria by a non-pore-forming mechanism. Because this neutrophil peptide possesses several distinct functional properties and because other antimicrobial peptides are chemoattractants, we sought to determine whether PR-39 was a chemoattractant for porcine leukocytes. The peptide was synthesized by the solid-phase method using t-Boc chemistry and purified by reversed-phase high-performance liquid chromatography. Leukocyte migration was assessed with the use of a 48-well microchemotaxis chamber. PR-39 induced the directed migration of neutrophils. The peak chemotaxis response occurred at 0.5-2 microM, which was slightly lower than the minimal inhibitory and bactericidal concentrations for PR-39. However, the peptide was not a chemoattractant for mononuclear cells. Truncation of PR-39 suggested that the neutrophil chemoattractant domain may be contained within the first 26 amino acid residues. Intracellular Ca2+ fluxes in response to PR-39 were monitored by flow cytometry and showed a transient increase that peaked at approximately 40 s and approached basal values by 4 min. However, in a Ca2+-free environment, the PR-39-induced Ca2+ increase was abrogated. Furthermore, PR-39 did not induce neutrophil chemotaxis in the absence of extracellular Ca2+, and pertussis toxin inhibited both neutrophil chemotaxis and Ca2+ mobilization. Taken together, these data suggest that PR-39 is a Ca2+-dependent chemoattractant of neutrophils. The finding of a neutrophil antibacterial peptide that is also a neutrophil chemoattractant is intriguing and may indicate an important role for PR-39 in inflammation.
Agglutination of Jelly Coat and Cortical Granule Components and the Block to Polyspermy in the Amphibian <i>Xenopus laevis</i> (1974)

A block to polyspermy in amphibians is established at fertilization by the conversion of the vitelline envelope to the fertilization envelope. In Xenopus laevis a major ultrastructural change in the envelope at fertilization is the appearance of an electron-dense layer, termed the F layer, between the envelope and the inner-most jelly coat layer, J(1). The F layer is derived, at least in part, from materials released from the cortical granules. Further definition of the origin and chemical nature of the F layer was sought by using isolated cortical granule (CG) exudate and jelly coat layer J(1). In double diffusion experiments, the isolated components interacted in an agglutination reaction producing a band of precipitation. The agglutination involved alpha-galactoside residues and metal ions (Ca(++)). Employing chemically modified jelly, we demonstrated that sulfhydryl-disulfide interchanges were not involved in the agglutination and, with (35)S-labeled jelly, that the agglutinating J(1) component possessed sulfate esters. Both the CG exudate and the J(1) components contained carbohydrate, as evidenced by their lectin reactivity. A number of ionic polymers, both natural and synthetic, were tested as chemical analogs of CG exudate and J(1); none gave an agglutination band. Dissolved jelly coat material from eggs of two different species of frogs agglutinated with CG exudate, while jelly from sea urchin eggs and hyaluronic acid from mammalian eggs did not. Thus, the agglutination reaction was chemically and phylogenetically specific. An electron-dense layer, similar to the F layer, formed on the outer of the vitelline envelope when jellied unfertilized eggs were immersed in CG exudate; such eggs were not fertilizable. We suggest that in Xenopus laevis, and perhaps other organisms as well, an agglutination type of reaction between cortical granule components and egg integuments is a participant in the structural and molecular events establishing a block to polyspermy.
A Highly Tunable Biocompatible and Multifunctional Biodegradable Elastomer (2012)

Biodegradable elastomers synthesized under mild conditions with highly tunable mechanical properties are described. These elastomeric biomaterials are biocompatible, exhibit minimal deformation following cyclical tensile loading, and permit tight control over the release kinetics of encapsulated bioactive molecules. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Experimental Investigations of Noise Control in Planetary Gear Set by Phasing (2014)

Now a days reduction of gear noise and resulting vibrations has received much attention of the researchers. The internal excitation caused by the variation in tooth mesh stiffness is a key factor in causing vibration. Therefore to reduce gear noise and vibrations several techniques have been proposed in recent years. In this research the experimental work is carried out to study the effect of planet phasing on noise and subsequent resulting vibrations of Nylon-6 planetary gear drive. For this purpose experimental set-up was built and trials were conducted for two different arrangements (i.e., with phasing and without phasing) and it is observed that the noise level and resulting vibrations were reduced by planet phasing arrangement. So from the experimental results it is observed that by applying the meshing phase difference one can reduce planetary gear set noise and vibrations.
Programmed Synthesis of Natural Product-like Non-standard Peptides Using the Translation System and Its Application (2010)

The translation machinery is generally designated to synthesize polypeptides consisting of 20 proteinogenic (natural) amino acids. Significantly, the ribosomal synthesis is employed in mRNA–template dependent manner, i.e. the sequence of mRNA dictates that of polypeptide according to the genetic code, enabling us to design the peptide sequence by simply preparing the corresponding mRNA or its DNA as the template for transcription. From the synthetic point of view, however, the fact that the useable monomers are limited to 20 proteinogenic amino acids is a serious restriction for the preparation of peptide library with high chemical diversities. In this review, we shall discuss an emerging new technology, referred to as genetic code reprogramming, that allows us to express natural product–like non–standard peptides using a reconstituted cell–free translation system. This technology will open a new avenue for the discovery of a new class of non–standard peptides.
SOME EFFECTS OF METHANE INHIBITION IN RUMINANTS (STEERS) (1972)

Four steers were used in a 4 × 4 latin square experiment to assess comparative antimethanogenic effects of including bromochloromethane (BCM) (5.5 g once daily), unsaturated fat (corn oil), and saturated fat (tallow) at 5% in experimental diets. Basic dietary ingredients were citrus pulp and beet pulp plus hay. Average daily gain (ADG) and ruminal parameters including soluble carbohydrate and total volatile fatty acid (TVFA) concentrations, VFA’s percentages, and pH were measured. Only BCM proved significantly (P < 0.05) antimethanogenic. Maximal inhibition occurred 0–6 hr post-feeding, by 15 hr inhibition was reduced by 50% and absent at 24 hr. Ruminal TVFA, pH, and average daily gain were not significantly affected (P > 0.05) by CH 4 inhibition, although average daily gain showed the following trend: BCM > saturated fat > unsaturated fat > control. Inhibition caused lower (P < 0.05) ruminal acetate, and higher (P < 0.05) propionate over 24 hr post-feeding, higher (P < 0.05) butyrate from 3–15 hr post-feeding, and consistent measurable quantities of valerate and isovalerate. Soluble CHO concentration was maximal 0–6 hr post-feeding.
Dielectric resonators raise your high-Q (2001)

High-quality resonating elements are the key to the function of most microwave circuits and systems. They are fundamental to the operation of filters and oscillators, and the performance of these circuits is primarily limited by the resonator quality factor. At microwave frequencies, the quality factor (Q) of metal transmission line resonant circuits is proportional to volume. As a result, waveguide structures are often employed to increase Q at the expense of size, weight, and cost. Dielectric resonators overcome these limitations due to the fact that their losses are dominated by dielectric loss (loss tangent), and only in small part to metallic losses (housing). Dielectric losses are improving constantly, whereas metal losses, with the exception of superconductors, have remained substantially the same. These resonators can be made to perform the same functions as waveguide filters, but are, in contrast, very small, stable and lightweight. The popularization of low-loss dielectric resonators roughly coincides with the miniaturization of many of the other associated elements of most microwave circuits. When taken together, these technologies permit the realization of small, reliable, lightweight and stable microwave systems.
Participación social y salud. Sujetos y actores de la democratización (2014)

In this essay I propose to review some related to social participation from my theoretical and practical experience in this field elements. Is not only about the participation in health, also about the participation of the people in general, at all levels referenced. For this, my reflections about the following topics are grouped: a) social participation or popular participation, b) rethink power and democracy, c) social subject and social actor, d) permanent construction of hegemony and counter-hegemony.
Computerized Doppler Tomography and Spectrum Analysis of Carotid Artery Flow (1981)

Contrast angiography remains the definitive study in the evaluation of atherosclerotic occlusive vascular disease. However, a safer technique for serial screening of symptomatic patients and for routine follow up is necessary. Computerized pulsed Doppler ultrasonic arteriography is a noninvasive technique developed by Miles(6) for imaging lateral, antero-posterior and transverse sections of the carotid artery. We [ill] this system with new software and hardware to analyze the three-dimensional blood flow data. The system now provides information about the location of the occlusive process in the artery and a semi-quantitative evaluation of the degree of obstruction. In addition, we interfaced a digital signal analyzer to the system which permits spectrum analysis of the pulsed Doppler signal. This addition has allowed us to identify lesions which are not yet hemodynamically significant.
The Expression of Phospho-AKT, Phospho-mTOR, and PTEN in Extrahepatic Cholangiocarcinoma (2009)

The AKT pathway is activated in a subset of extrahepatic cholangiocarcinoma. Elevated PTEN expression correlates with longer survival. Quantitative data obtained by multiplex tissue immunoblotting may provide additional information than assessment of immunohistochemistry alone. Quantitative analysis of PTEN, PTEN/p-AKT and PTEN/p-mTOR shows differences in survival by univariate analysis.
Small-molecule inhibitors of dimeric transcription factors: Antagonism of protein–protein and protein–DNA interactions (2012)

Transcription factors are DNA-binding proteins that – usually in combination with other proteins to form the pre-initiation complex (PIC) – regulate the transcription of specific DNA sequences (genes) into mRNA by controlling the recruitment of RNA polymerase II. Constitutive activation of transcription factors can lead to a variety of cancers, and are, therefore, important therapeutic targets. However, in stark contrast to targeting enzyme active sites, disruption of protein–protein or protein–DNA interactions involved in the transcriptional machinery is particularly challenging owing to the large interfacial areas involved, a lack of obvious binding sites and often non-contiguous contact points. Especially problematic for the development of small-molecules is the need by such agents to overcome the large free energy of association between protein–protein and, to a lesser extent, protein–DNA interfaces. Nevertheless, recent years have seen considerable success in this area of medicinal chemistry, cementing the notion that disruption of such interactions is feasible with small-molecule, drug-like compounds. We discuss, in particular, the disruption of dimeric transcription factors, such as STAT3–STAT3, c-Myc–Max and c-Jun–c-Fos (AP-1), with small-molecules that block their protein–protein interactions or their interactions with DNA.