gebhart/patrepeval huggingface dataset:
<i>Actinobacillus actinomycetemcomitans</i> Immunosuppressive Protein Is a Member of the Family of Cytolethal Distending Toxins Capable of Causing a G2 Arrest in Human T Cells (1999)

We have previously shown that Actinobacillus actinomycetecomitans produces an immunosuppressive factor (ISF) capable of impairing human lymphocyte function by perturbing cell cycle progression. We now report that ISF is the product of the cdtB gene, one of three genes encoding the family of cytolethal distending toxins (Cdt). The ISF polypeptide exhibits >/=95% identity with Hemophilus ducreyi CdtB protein and </=60% homology with Escherichia coli or Campylobacter jejuni CdtB. Pretreatment of PHA-activated lymphocytes with 5-25 ng ISF results in G2 arrest of CD4+ and CD8+ T cells. Similarly, treatment of HeLa cells results in G2 arrest and cell elongation and distension. However, lymphocytes are at least 5 times more sensitive to ISF than HeLa cells and do not undergo the elongation and distension that characterizes interactions of Cdts with cell lines. ISF-treated lymphocytes express normal cyclin A and B1 levels, but contain reduced levels of cell cycle-dependent kinase-1 (Cdk1). Additionally, the majority of Cdk1 is in the hyperphosphorylated, inactive, form. In contrast, PHA-induced G2 cells contain elevated levels of the hypophosphorylated, active Cdk1. Failure of ISF-treated cells to dephosphorylate Cdk1 is not associated with decreased availability of Cdc25. These studies suggest that the CdtB protein alone is capable of inducing G2 arrest in lymphocytes and cell cycle arrest, elongation, and distension of HeLa cells. Our studies also suggest that lymphocytes may be primary targets for A. actinomycetemcomitans CdtB (ISF) and possibly for other Cdt family members as well. Thus, Cdts may function to impair host immunity and contribute to the pathogenesis of disease associated with Cdt-producing organisms.
Two generations of synthetic membranes for biological/medical applications (2004)

Two generations of amphiphilic membranes synthesized in our laboratories for biological/medical applications are highlighted. Recent results obtained with representative 2nd generation membranes designed specifically for immunoencapsulation of pancreatic islets and cells are summarized. The latter tri-component membranes comprise well-defined hydrophilic poly(ethylene glycol) and hydrophobic/oxyphilic polydimethylsiloxane segments crosslinked by oxyphilic polycyclosiloxane domains, which also provide reinforcement. Select membranes are demonstrated to allow the diffusion of glucose and insulin but prevent the permeation of immunoglobulin G. These membranes remain permeable despite long incubation in IgG solution.
Interleukin-10 Abrogates the Inhibition of Epstein-Barr Virus–Induced B-Cell Transformation by Memory T-Cell Responses (1998)

In vitro infection of human B lymphocytes by Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalized lymphoblastoid cell lines. The virus was found to encode a homologue of the pleiotropic cytokine interleukin-10 (IL-10), which has wide-ranging effects on the immune system. We investigated the effect of human IL-10 (hIL-10) and viral IL-10 (vIL-10) on EBV-specific immunological memory, as assessed by the inhibition of EBV-induced B-cell transformation by the autologous T cells. We found that IL-10 abrogates the inhibitory capacity of T cells. This IL-10 effect is mediated through suppression of T-cell activation-induced IL-2 and interferon-γ production and through a direct enhancement of EBV-infected B-cell growth.
A WEAK STRUCTURAL TEXTURE ANALYSIS TECHNIQUE TO WAVE HEIGHTS FOR OCEAN WAVES IMAGE (1988)

A weak structural texture analysis technique to wave heights for ocean waves image is presented. Based upon the representation of ocean waveform by relational trees, the absolute peak heights, the relative peak heights and the peak width are extracted. The histogram of peak heights for given width is obtained. In accordance with it, a weak structural texture measure
The Reactivity of the Methyl Group in 2-Methyl-3-nitronaphthalene<sup>1</sup> (1954)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Reactivity of the Methyl Group in 2-Methyl-3-nitronaphthalene1Leonard E. Miller, W. W. Hanneman, W. L. St. John, and R. R. SmebyCite this: J. Am. Chem. Soc. 1954, 76, 1, 296–297Publication Date (Print):January 1, 1954Publication History Published online1 May 2002Published inissue 1 January 1954https://pubs.acs.org/doi/10.1021/ja01630a093https://doi.org/10.1021/ja01630a093research-articleACS PublicationsRequest reuse permissionsArticle Views590Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Recent advances in non-surgical and surgical periodontics. (2002)

The role of periodontal surgery in the management of periodontal diseases has continued to develop and benefit from advances in clinical as well as basic science research. The periodontist now has a wide variety of surgical treatment options to help patients who present with the manifestations of periodontal disease. This article looks at the surgical treatment modalities that are currently being used in clinical practice. In addition, it looks at the role of genetic testing in the overall patient management sequence.
Conditional DNA repair mutants enable highly precise genome engineering (2014)

Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial genome editing. The efficient application of this technique requires the inactivation of the endogenous methyl-directed mismatch repair system that in turn leads to a drastically elevated genomic mutation rate and the consequent accumulation of undesired off-target mutations. Here, we present a novel strategy for mismatch repair evasion using temperature-sensitive DNA repair mutants and temporal inactivation of the mismatch repair protein complex in Escherichia coli. Our method relies on the transient suppression of DNA repair during mismatch carrying oligonucleotide integration. Using temperature-sensitive control of methyl-directed mismatch repair protein activity during multiplex genome engineering, we reduced the number of off-target mutations by 85%, concurrently maintaining highly efficient and unbiased allelic replacement.
Paying for the Skills, Knowledge, and Competencies of Knowledge Workers (1995)

Companies will see a "tremendous increase" in experimentation with competency-based pay plans for knowledge workers during the next ten years. While solid outcomes research is still lacking, design choices are becoming clearer.
Counterfactual reasoning about intent for interactive navigation in dynamic environments (2015)

Many modern robotics applications require robots to function autonomously in dynamic environments including other decision making agents, such as people or other robots. This calls for fast and scalable interactive motion planning. This requires models that take into consideration the other agent's intended actions in one's own planning. We present a real-time motion planning framework that brings together a few key components including intention inference by reasoning counterfactually about potential motion of the other agents as they work towards different goals. By using a light-weight motion model, we achieve efficient iterative planning for fluid motion when avoiding pedestrians, in parallel with goal inference for longer range movement prediction. This inference framework is coupled with a novel distributed visual tracking method that provides reliable and robust models for the current belief-state of the monitored environment. This combined approach represents a computationally efficient alternative to previously studied policy learning methods that often require significant offline training or calibration and do not yet scale to densely populated environments. We validate this framework with experiments involving multi-robot and human-robot navigation. We further validate the tracker component separately on much larger scale unconstrained pedestrian data sets.
From RavensbrÃ¼ck to Algiers and Noisy-le-Grand: Dialogues with Deportation (2004)

Faced with a troubled past, national collectivities can negotiate identities through iconic figures. Prescient hero Charles de Gaulle and later Resistance martyr Jean Moulin played this role in France in the decades after World War II. More recently, other individuals from the same generation have come to the fore as exemplary actors through whom the French enact reconciliation with their nation’s wartime history. Marc Bloch, a Jew executed for his Resistance activity, has become a figure who allows French republicans to work their way out of what Henry Rousso terms the obsessive phase of the Vichy Syndrome.
The Block-Oriented Computer (1969)

LSI will have a profound effect on the design of computer systems ranging from evolutionary changes in implementation to revolutionary changes in basic architecture. This paper discusses a computer composed of an array of processors. Entire wafers are used, rather than wafers diced into chips. The computer utilizes software to substitute process elements for the purpose of compensating for imperfect yield on the wafer. The computer organization possesses a property called block orientation which permits the use of whole LSI wafers with low yields without requiring a secondary metalization manufacturing step. Through the use of a unique computing algorithm, the parallelism and communication problems inherent to array processors are minimized. Processing systems of the sort discussed can possess a self-healing capability.
(3-14) Numerical Simulations of Spray Formation Process in High and Low Ambient Pressures Using a Swirl-Type Injector((FS-1)Fuel Sprays 1-Gasoline sprays) (2001)

Predictions of the mixture formation process inside gasoline DI engines are strongly required to improve both the fuel consumption rate and the exhaust emissions. Swirl-type injectors are commonly used for gasoline DI engines, as its spray characteristics are favorable for gasoline DI engines. The spray formation of a swirl-type injector consists of a liquid sheet and droplets that arise from the breakup of the liquid sheet. Numerical simulations of free sprays formed by a swirl-type injector are carried out on the basis of a method of DDM (Discrete Droplet Model) with employing different models for the breakup of the liquid sheet and that of droplets downstream. The breakup of the liquid sheet is modeled by Reitz's wave breakup model while that of droplets downstream adopts TAB (Taylor Analogy Breakup) model. Schematic diagram is illustrated in Figure 1. In this study, the boundary condition of the ambient pressure is set at two values ; a negative pressure and a high pressure. The droplet deformation calculated by the breakup model is incorporated into the drag force term to take the influence of the drag variations into account. The results of calculated parcels on centered vertical cross-section are shown in Figure 2. Figure 2(a) is the result assuming that the drag force is of a rigid sphere (without droplet deformation), while Figure 2(b) is the result assuming that the drag force is of an ellipsoid (with droplet deformation). As a result, by taking the drag force variation due to deformation of droplets into account, calculations under high ambient pressure can reproduce the change of spray shape. The drag force of droplets and the pressure difference of spray inside and outside cone contribute to the change of the spray shape. This pressure difference is small when the ambient pressure is low.
Electron Microscopy Of Subcellular Dynamics (1989)

Current Trends in the Electron Microscopic Analysis of Dynamic Processes in the Field of Cell and Molecular Biology (H. Plattner). Self-Assembly of Crystalline Bacterial Cell Surface Layers (S-Layers) (U.B. Sleytr and P. Messner). Gap Junctions: Correlated Electrophysiological Recordings and Ultrastructural Analysis by Fast Freezing and Freeze-Fracturing (D.F. Hulser, D. Paschke, and J. Greule). Analysis of Irregular Distributions and Dynamics of Membrane Components by Computer-Aided Image Recognition and Statistical Evaluation (S.W. Hui). Lipid Phase Transitions and Micro-Domains in Biomembranes and Their Possible Relevance for Biological Processes (A.J. Verkleij). Ultrastructural Analysis of Biological Membrane Fusion and a Tentative Correlation with Biochemical and Biophysical Aspects (G. Knoll and H. Plattner). Analysis of Dynamic Cell Processes by Rapid Freezing and Freeze Substitution (E. Morgenstern and L. Edelmann). Topography and Internalization of Cell-Surface Receptors as Analyzed by Affinity- and Immuno-Labeling Combined with Surface Replication and Ultrathin Sectioning Techniques (H. Robenek). Enzymatic Labeling of Cell-Surface Glycoproteins as an Autoradiographic Probe for Morphometric Analysis of Intracellular Membrane Traffic (L. Thilo and C. de Chastellier). Low-Temperature Embedding and Cryosectioning in the Immunocytochemical Study of Membrane Recycling (D. Brown). Pre-embedding Labeling Techniques Applicable to Intracellular Binding Sites (M. Pavelka and A. Ellinger). Organizational Principles of the Golgi Apparatus: Contributions from Postembedding Gold Labeling Techniques (J. Roth and D.J. Taatjes). Neoglycoproteins as Tools to Analyze Endogenous Lectins and Glycoconjugates (M. Monsigny, J. Hubert, A. Obrenovitch, and J. Schrevel). Interaction between Cytoskeleton and Plasma Membrane in Virus Morphogenesis (W. Bohn). Scanning Electron Microscopy of Cell Surface Antigens Labeled with Colloidal Gold Markers (E. de Harven). Immunocytochemistry of Contractile and Cytoskeletal Proteins (J.V. Small). Analysis of Complex Three-Dimensional Structures Involved in Dynamic Processes by High Voltage Electron Microscopy and Low Voltage High Resolution Scanning Electron Microscopy (H. Ris and J.B. Pawley). X-Ray Microanalysis of Ion Changes during Fast Processes in Cells, as Exemplified by Trichocyst Exocytosis of Paramecium caudatum (M. Schmitz and K. Zierold). Correlated Electrostimulation and Electron Probe X-Ray Microanalysis of Heart Muscle Cells (M.F. Wendt-Gallitelli and G. Isenberg). Index.
Techniques and cost effectiveness of enteroatmospheric fistula isolation . (2010)

Successful isolation of enteroatmospheric fistulae using a NPWT system has the potential to lower healthcare system costs by allowing for earlier hospital discharge.
Prevention of Postoperative Adhesions in Rabbits with Streptococcal Metabolites. (1950)

The results in this series of animals were sufficiently encouraging as to justify the further investigation of streptokinase-streptodornase in the prevention of experimental postoperative adhesions.
The Ergot Alkaloids. The Structure of Lysergic Acid (1936)

Article MetricsDownloadsCitationsNo data available.01JulAugSepOctNovDec90Total6 Months12 MonthsTotal number of downloads for the most recent 6 whole calendar months.
Efficient magnetic resonance imaging methods for automated quantitation of magnetic resonance parameters from multiple samples (1997)

Magnetic resonance imaging methods have been developed which simultaneously measure the bulk magnetic resonance parameters of many samples contained in stacks of multi-well sample plates using either a 2.35 T, 31 cm or a 2 T, 100 cm horizontal bore magnet each with a quadrature ‘birdcage’ resonator. Computerized automatic edge detection software detects the position of each well within the first spin-echo image which can then determine the mean magnetic resonance parameters of the sample in each well. An example is given of the automated quantitation of T1, T2 and M0 values for 2% (w/v) agar gel in four plates each with 30 wells per plate (120 samples in total). It was found that the maximum number of samples that can be analysed is limited by the homogeneity of the probe B1 field and the B0 field of the magnet. The applicability of this technology for future applications is discussed. © 1977 John Wiley & Sons, Ltd.
Chained declustering: a new availability strategy for multiprocessor database machines (2002)

A new strategy for increasing the availability of data in multiprocessor, shared-nothing database machines is presented. This technique, termed chained declustering, is demonstrated to provide superior performance in the event of failures while maintaining a very high degree of data availability. Furthermore, unlike most earlier replication strategies, the implementation of chained declustering requires no special hardware and only minimal modifications to existing software.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pitfalls of the Biographer (1933)

Research Article| March 01 1933 Pitfalls of the Biographer Rupert Hughes Rupert Hughes Search for other works by this author on: This Site PubMed Google Scholar Pacific Historical Review (1933) 2 (1): 1–33. https://doi.org/10.2307/3633424 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Rupert Hughes; Pitfalls of the Biographer. Pacific Historical Review 1 March 1933; 2 (1): 1–33. doi: https://doi.org/10.2307/3633424 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search nav search search input Search input auto suggest search filter All ContentPacific Historical Review Search This content is only available via PDF. Copyright 1933 The Arthur H. Clark Company Article PDF first page preview Close Modal You do not currently have access to this content.
Pressure and pressure gradient fiber-optic lever hydrophones (1983)

Fiber-optic levers have been found useful in the design of intracardiac pressure transducers, low-displacement biological measurements, and more generally as noncontact optical proximity detectors. In this paper the utilization of a bifurcated fiber-optical lever in the design of hydrophones of the pressure and pressure gradient types has been considered. This approach leads to simplicity, low cost, and relative ease in implementation. Some optical and acoustical considerations are given to establish the validity of the experimental data presented. It is shown that with little effort these devices can provide acoustic sensitivities at least equivalent to those of piezoceramic elements within the same frequency range and can generate acceptable directivity patterns in the pressure and pressure gradient modes.
Abstract 287: Specific delivery of immunostimulatory RNA via nanoparticles blocks growth of primary and disseminated ovarian tumors (2015)

Abstract One important mechanism of immune evasion is the accumulation of tumor-infiltrating regulatory T cells creating an immunosuppressive microenvironment in situ. Many new therapies target immune checkpoint receptors, CTLA-4 and PD-1, on immunosuppressive T-cells to reverse tumor immune evasion. Small interfering RNAs (siRNAs) are double stranded, sequence-specific inhibitors of gene expression. The paradigm for using siRNA to block cancer is to target mRNA sequences of oncogenes. Several limitations have suppressed the use of siRNA in human cancer therapy, such as off-target effects and lack of tumor-specific delivery. Additionally, certain siRNA sequence motifs activate toll-like receptors that induce the systemic innate immune response, including induction of interferon and interleukin-6 release that is traditionally considered as an undesirable clinical outcome. The hypothesis proposed here is to control the innate immune response by directing it only to tumors and not other organs by delivering an immunostimulatory siRNA (isRNA) directly to tumors. An isRNA designed to silence no human genes was synthesized as an unmodified triphosphate 19-mer double-stranded RNA with a GUGA motif on the 5′ antisense end. This isRNA was delivered into human ovarian tumors grown from SK-OV-3 cells surgically implanted into the ovaries of immunosuppressed mice by an L1CAM-targeted elastin-like polypeptide (ELP-L) nanoparticle. The isRNA/ELP-L nanocomplex was dosed into ten mice by i.p. injection 14 times at 10 mg/kg with respect to the isRNA over 28 days. The isRNA was delivered to the tumors, not to liver, spleen, heart, or lungs. The total tumor mass and number of disseminated nodules both decreased by 50% with respect to vehicle control. Six ovaries harboring tumors were resected from isRNA/ELP-L nanocomplex-dosed mice and each displayed elevated levels of interferon alpha and interleukin 6 mRNA as determined by RT-PCR. Mean AST, ALT and BUN serum biomarkers of liver and kidney function were elevated by 68%, 128% and 14%, respectively, compared to vehicle dosed mice; none of which were outside average normal levels. These results indicate that specifically targeting isRNA to tumors using a targeted nanoparticle may provide an effective therapy for ovarian cancer, either alone or in combination with agents that affect immune check point pathways in tumors. Citation Format: Thomas Primiano, Bey-ih Chang. Specific delivery of immunostimulatory RNA via nanoparticles blocks growth of primary and disseminated ovarian tumors. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 287. doi:10.1158/1538-7445.AM2015-287
Graph cuts based left atrium segmentation refinement and right middle pulmonary vein extraction in C-arm CT (2013)

Automatic segmentation of the left atrium (LA) with the left atrial appendage (LAA) and the pulmonary vein (PV) trunks is important for intra-operative guidance in radio-frequency catheter ablation to treat atrial fibrillation (AF). Recently, we proposed a model-based method<sup>1, 2</sup> for LA segmentation from the C-arm CT images using marginal space learning (MSL).<sup>3</sup> However, on some data, the mesh from the model-based segmentation cannot exactly fit the true boundary of the left atrium in the image since the method does not make full use of local voxel-wise intensity information. Furthermore, due to the large variations of the PV drainage pattern, extra right middle pulmonary veins are not included in the LA model. In this paper, a graph-based method is proposed by exploiting the graph cuts method to refine results from the model-based segmentation and extract right middle pulmonary veins. We first build regions of interest to constrain the segmentation. The region growing method is used to construct graphs within the regions of interest for the graph cuts optimization. The graph cuts optimization is then performed and newly segmented foreground voxels are assigned into different parts of the left atrium. For the extraction of right middle pulmonary veins, occasional false positive PVs are removed by examining multiple criteria. Experiments demonstrate that the proposed graph-based method is effective and efficient to improve the LA segmentation accuracy and extract right middle PVs.