gebhart/patrepeval huggingface dataset:
Human antiglioma monoclonal antibodies from patients with astrocytic tumors (1992)

The current management of malignant gliomas is unsatisfactory compared to that of other solid tumors; the expected median survival period is less than 1 year with the patient undergoing conventional surgery, radiotherapy, and chemotherapy treatment. Immunological reagents could be a useful adjunct. Human monoclonal antibodies derived from patients with astrocytic tumors might recognize subtle antigenic specificities that would differ from those recognized by xenogeneic (murine) systems. Five hybridomas, designated as BT27/1A2, BT27/2A3, BT32/A6, BT34/A5, and BT54/B8, were produced from the fusion of peripheral blood lymphocytes of four patients with astrocytic tumors to the human myeloma-like cell line TM-H2-SP2. This cell line has a 46, XX karyotype and is negative for hypoxanthine guanine phosphoribosyltransferase. All five human monoclonal antibodies produced 2.4 to 44 micrograms/ml of immunoglobulin M, had a similar but not identical pattern of reactivity against a panel of human tumor cell lines, and failed to react with normal human astrocytes. Labeling of four neuroectodermal tumor explant cultures by BT27/2A3 was demonstrated by flow cytometry. Karyotyping of three of the five hybridomas demonstrated that two were pseudodiploid (2-3n) and one hypodiploid (less than 2n). The monoclonality of the hybridomas was evaluated by Southern blot analysis of JH gene rearrangements, revealing two types of rearrangements for each hybridoma, both consistent with monoclonality. Preliminary antigen characterization indicated that at least four of the five human monoclonal antibodies were directed to cell-surface glycolipids.
Copolymers, Polyblends, and Composites (1975)

The basic element of fibrous material, microfibril, is composed of alternating amorphous layers and crystalline blocks. The stacked lamellae of the spherulitic or cylindritic starting material are transformed into densely packed and aligned microfibrillar bundles (fibrils) of the highly oriented fibrous material. During the necking process, the few tie molecules connecting the stacked lamellae yield the interfibrillar tie molecules that connect the microfibrils laterally. Their number is small compared with that of intrafibrillar tie molecules, but it increases substantially during plastic deformation of the fibrous structure when the fibrils are sheared and axially displaced. Fibril shearing displaces the microfibrils in the fiber direction and enormously extends the interfibrillar tie molecules by chain unfolding without any substantial change in microfibrillar structure.
A review of radio frequency identification technology for the anatomic pathology or biorepository laboratory: Much promise, some progress, and more work needed (2011)

Patient safety initiatives throughout the anatomic laboratory and in biorepository laboratories have mandated increasing emphasis on the need for accurately identifying and tracking biospecimen assets throughout their production lifecycle and for archiving/retrieval purposes. However, increasing production volume along with complex workflow characteristics, reliance on manual production processes, and required asset movement to disparate destinations throughout asset lifecycles continue to challenge laboratory efforts. Radio Frequency Identification (RFID) technology, use of radio waves to communicate data between electronic tags attached to objects and a reader, shows significant potential to facilitate and overcome these hurdles. Advantages over traditional barcode labeling include readability without direct line-of-sight alignment to the reader, ability to read multiple tags simultaneously, higher data storage capacity, faster data transmission rate, and capacity to perform multiple read-writes of data to the tag. Most importantly, use of radio waves decreases the need to manually scan each asset, and at each step, identification or tracking event is needed. Temperature monitoring by on-board sensors and three-dimensional position tracking are additional potential benefits of using RFID technology. To date, barriers to implementation of RFID systems in the anatomic laboratory include increased associated costs of tags and readers, system software, data security concerns, lack of specific data standards for stored information, and potential for technological obsolescence during decades of specimen storage. Novel RFID production techniques and increased production capacity are projected to lower costs of some tags to a few cents each. Potentially, information security concerns can be addressed by techniques such as shielding, data encryption, and tag pseudonyms. Commitment by stakeholder groups to develop RFID tag data standards for anatomic pathology and biorepository laboratories could avoid or mitigate the "islands of data" dilemma presented by barcode usage where there are innumerable standards and a consequent paucity of hardware or software "plug and play" interoperability. Work remains to be done to establish the durability and appropriate shielding of individual tag types for use in harsh laboratory environmental conditions, and for long-term archival storage. Finally, given the requirements for long-term storage of biospecimen assets, consideration should be given to ways of mitigating data isolation due to eventual technological obsolescence of a particular RFID technology or software.
Camelysin Is a Novel Surface Metalloproteinase from <i>Bacillus cereus</i> (2003)

Bacillus cereus frequently causes food poisoning or nosocomial diseases. Vegetative cells express the novel surface metalloproteinase camelysin (casein-cleaving metalloproteinase) during exponential growth on complex, peptide-rich media. Camelysin is strongly bound to the cell surface and can be solubilized only by detergents or butanol. Camelysin spontaneously migrates from the surface of intact bacterial cells to preformed liposomes. The complete sequence of the camelysin-encoding gene, calY, was determined by reverse PCR on the basis of the N-terminal sequence and some internal tryptic cleavage peptides. The calY gene codes for a polypeptide of 21.569 kDa with a putative signal peptide of 27 amino acids (2.513 kDa) preceding the mature protein (19.056 kDa). Although the predicted amino acid sequence of CalY does not exhibit a typical metalloprotease consensus sequence, high-pressure liquid chromatography-purified camelysin contains one zinc ion per protein molecule. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and tryptic peptide mass fingerprinting confirmed the identity of this zinc-binding protein as CalY. Disruption of the calY gene results in a strong decrease in the cell-bound proteolytic activity on various substrates.
ART (1940)

Art is not an aesthetic but a rhetorical activity. (Ananda Coomaraswamy) The Incarnation may be said to have for Its object the drawing of men from misery to happiness. Being the act of God It is the greatest of all rhetorical acts and therefore the greatest of all works of art. And as from the fatherhood of God all paternity is named in heaven and earth, so from His creative power all art is named. In the Incarnation we do not only know a fact of history or a truth of religion; we behold a work of art, a thing made. As a fact of history It is the most interesting and illuminating of all historical happenings. As a truth of religion It is of primary and fundamental importance. But It is as a work of art that It has a saving power, power to persuade, power to heal, power to rescue, power to redeem. But the word ‘art,’ in spite of the obsequious worship which the modern world gives to the works of painters and sculptors and musicians, is not a holy word in these days. Art, the word, which primarily means skill and thus human skill and thus human skill in doing and making, has, in literary circles and among the upper classes, come to mean only the fine arts, and the fine arts have ceased to be rhetorical and are now exclusively aesthetic; they aim only to give pleasure.
Drug-Drug Interaction Study of Ketoconazole and Ritonavir-Boosted Saquinavir (2008)

Saquinavir, a potent human immunodeficiency virus protease inhibitor, is extensively metabolized by CYP3A4. Saquinavir is coadministered with ritonavir, a strong CYP3A4 inhibitor, to boost its exposure. Ketoconazole is a potent CYP3A inhibitor. The objectives of this study were to investigate the effect of ketoconazole on the pharmacokinetics of saquinavir/ritonavir and vice versa using the approved dosage regimens of saquinavir/ritonavir at 1,000/100 mg twice daily and ketoconazole at 200 mg once daily. This was an open-label, randomized two-arm, one-sequence, two-period crossover study in healthy subjects. In study arm 1, 20 subjects received saquinavir/ritonavir treatment alone for 14 days, followed in combination with ketoconazole treatment for 14 days. In arm 2, 12 subjects received ketoconazole treatment for 6 days, followed in combination with saquinavir/ritonavir treatment for 14 days. The pharmacokinetics were assessed on the last day of each treatment (days 14 and 28 in arm 1 and days 6 and 20 in arm 2). The exposures C(max) and the area under the concentration-time curve from 0 to 12 h (AUC(0-12)) of saquinavir and ritonavir with or without ketoconazole were not substantially altered after 2 weeks of concomitant dosing with ketoconazole. The C(max) and AUC(0-12) of ketoconazole, dosed at 200 mg once daily, were increased by 45% (90% confidence interval = 32 to 59%) and 168% (90% confidence interval = 146 to 193%), respectively, after 2 weeks of concomitant dosing with ritonavir-boosted saquinavir (1,000 mg of saquinavir/100 mg of ritonavir given twice daily). The greater exposure to ketoconazole when given in combination with saquinavir/ritonavir was not associated with unacceptable safety or tolerability. No dose adjustment for saquinavir/ritonavir (1,000/100 mg twice daily) is required when coadministered with 200 mg of ketoconazole once daily, and high doses of ketoconazole (>200 mg/day) are not recommended.
Rectangular-apertured micro-Fresnel lens arrays fabricated by electron-beam lithography (1987)

Rectangular-apertured micro-Fresnel lens arrays are proposed. These lens arrays have been fabricated by an electron-beam writing system specially developed for the fabrication of microoptical devices. It is experimentally demonstrated that the lens arrays showed uniform focusing characteristics, and that each lens exhibited a diffraction-limited focusing characteristic with efficiency of 74%.
Protecting Key Exchange and Management Protocols Against Resource Clogging Attacks (1999)

Many cryptographic key exchange and management protocols involve computationally expensive operations, such as modular exponentiations, and are therefore vulnerable to resource clogging attacks. This paper overviews and discusses the basic principles and the rationale behind an anti-clogging mechanism that was originally designed and proposed to protect the Photuris Session Key Management Protocol against resource clogging attacks. The mechanism was later approved by the IETF IPsec WG to be included into the Internet Key Management Protocol (IKMP) or Internet Key Exchange (IKE) protocol respectively. The paper introduces and discusses the Photuris anti-clogging mechanism, derives some design considerations, and elaborates on possibilities to use similar techniques to improve an existing HTTP state management protocol and to protect TCP/IP implementations against TCP SYN flooding attacks.
BRAIN, SKELETAL MUSCLE AND PLATELET HOMOGENATE MITOCHONDRIAL FUNCTION IN PARKINSON'S DISEASE (1992)

The recent discovery of mitochondrial complex I deficiency in the substantia nigra of patients with idiopathic Parkinson's disease has provided new understanding into the possible mechanisms that may underlie this neurodegenerative disorder. The biochemical defect is identical to that induced in humans, primates and mice exposed to the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. We have studied mitochondrial respiratory chain function in various brain regions, in skeletal muscle and in blood platelets from patients with idiopathic Parkinson's disease and from matched controls. We provide evidence suggesting that the complex I deficiency in Parkinson's disease is limited to the brain and that this defect is specific for the substantia nigra. The tissue specificity of the complex I deficiency in Parkinson's disease and its localization to the substantia nigra support the proposition that complex I deficiency may be directly involved in the cause of dopaminergic cell death in Parkinson's disease. An understanding of the molecular basis of this biochemical defect will provide valuable insight into the cause of Parkinson's disease. Our findings of normal mitochondrial function in platelet homogenates suggests that this tissue cannot be used to develop a 'diagnostic test' for Parkinson's disease.
<i>In vivo</i> metal‐ion release from porous titanium‐fiber material (1984)

Dense and porous Ti, Ti-alloy, and stainless steel specimens were implanted in canine trabecular bone. After 6 and 12 months the specimens were retrieved together with bone tissue immediately adjacent. The trace metal content in the tissue samples was determined using neutron activation analysis, differential pulse polarography, graphite furnace atomic-absorption spectrophotometry, electron microprobe analysis, and laser microprobe analysis. The results are discussed in view of (i) the release of Ti ions, which is larger for porous than for bulk specimens, (ii) the various artifacts arising in electron microprobe (EMP) and laser microprobe mass analysis (LAMMA) determination of compositional gradients of trace metal-ion content in bone tissue, (iii) the absence of measurable quantities of V in bone tissue, and (iv) the difference in local tissue accumulation between Ti, released from Ti specimens, and Ni, released from stainless steel specimens.
Netscape ONE Sourcebook (1997)

NETSCAPE ONE SOFTWARE Netscape Communicator SuiteSpot Creating a Netscape Web Site NETSCAPE ONE TECHNOLOGIES Netscape Meets ActiveX JavaScript Internet Foundation Classes LiveConnect AppFoundry NETSCAPE ONE OBJECT SUPPORT CORBA, IIOP, and the Web Java Objects Appendix Index.
Estimation of wave phase speed and nearshore bathymetry from video imagery (2000)

A new remote sensing technique based on video image processing has been developed for the estimation of nearshore bathymetry. The shoreward propagation of waves is measured using pixel intensity time series collected at a cross‐shore array of locations using remotely operated video cameras. The incident band is identified, and the cross‐spectral matrix is calculated for this band. The cross‐shore component of wavenumber is found as the gradient in phase of the first complex empirical orthogonal function of this matrix. Water depth is then inferred from linear wave theory's dispersion relationship. Full bathymetry maps may be measured by collecting data in a large array composed of both cross‐shore and longshore lines. Data are collected hourly throughout the day, and a stable, daily estimate of bathymetry is calculated from the median of the hourly estimates. The technique was tested using 30 days of hourly data collected at the SandyDuck experiment in Duck, North Carolina, in October 1997. Errors calculated as the difference between estimated depth and ground truth data show a mean bias of −35 cm (rms error = 91 cm). Expressed as a fraction of the true water depth, the mean percent error was 13% (rms error = 34%). Excluding the region of known wave nonlinearities over the bar crest, the accuracy of the technique improved, and the mean (rms) error was −20 cm (75 cm). Additionally, under low‐amplitude swells (wave height H ≤ 1 m), the performance of the technique across the entire profile improved to 6% (29%) of the true water depth with a mean (rms) error of −12 cm (71 cm).
Enhancing write I/O performance of disk array RM2 tolerating double disk failures (2003)

With a large number of internal disks and the rapid growth of disk capacity, storage systems become more susceptible to double disk failures. Thus, the need for such reliable storage systems as RAID6 is expected to gain in importance. However RAID6 architectures such as RM2, P+Q, EVEN-ODD, and DATUM traditionally suffer from a low write I/O performance caused by updating two distinctive parity data associated with user data. To overcome such a low write I/O performance, we propose an enhanced RM2 architecture which combines RM2, one of the well-known RAID6 architectures, with a Lazy Parity Update (LPU) technique. Extensive performance evaluations reveal that the write I/O performance of the proposed architecture is about two times higher than that of RM2 under various I/O workloads with little degradation in reliability.
Electrochemistry of InSb as a Li Insertion Host: Problems and Prospects (2001)

Ballmilling of In and Sb has been used to produce InSb for use in electrochemical and in situ X-ray diffraction studies (XRD) of ethylene carbonate:diethyl carbonate/InSb cells. The cell capacity decays rapidly when cycled between 0 and 1.3 V, while the capacity reduction is less pronounced when cycling is restricted to the 0.65-1.4 V range. In situ XRD studies reveal that and In are formed during the first plateau (above 0.65 V), according to the reaction The indium product subsequently reacts with Li forming the phases InLi and in sequence. When cells are cycled above 0.6 V (i.e., in the absence of formation) capacity retention improves significantly, remaining relatively constant near 250 mAh/g. Detailed in situ XRD studies of these cells suggest that 0.27 Li atoms per InSb may be intercalated during a sharp drop in the cell potential, according to the reaction This intercalation accounts for only a small (about 30 mAh/g) fraction of the overall capacity of 680 mAh/g. Consequently, it appears that the reactivity of In and Sb with Li, not the structure type, determines the reaction path. Therefore, InSb is not an attractive intercalation host for Li, in contrast to the claims made in the literature. © 2001 The Electrochemical Society. All rights reserved.
LT-RTPG: a new test-per-scan BIST TPG for low switching activity (2006)

A new built-in self-test (BIST) test pattern generator (TPG) design, called low-transition random TPG (LT-RTPG), is presented. An LT-RTPG is composed of a linear feedback shift register (LFSR), a /spl kappa/-input AND gate, and a T flip-flop. When used to generate test patterns for test-per-scan BIST, it decreases the number of transitions that occur during scan shifting and, hence, decreases switching activity during testing. Various properties of LT-RTPGs are identified and a methodology for their design is presented. Experimental results demonstrate that LT-RTPGs designed using the proposed methodology decrease switching activity during BIST by significant amounts while providing high fault coverage.
Metal-dependent Self-assembly of Protein Tubes from Escherichia coli Glutamine Synthetase (1999)

Escherichia coli glutamine synthetase (GS) is a dodecameric assembly of identical subunits arranged as two back-to-back hexagonal rings. In the presence of divalent metal ions, the dodecamers "stack" along their six-fold axis of symmetry to yield elongated tubes. This self-assembly process provides a useful model for probing metal-dependent protein-protein interactions. However, no direct spectroscopic or structural data have confirmed the identity of the ligands to the shared metal ions in "stacked" GS. Here, 9-GHz Cu(2+) EPR studies have been used to probe the ligand structure and stoichiometry of the metal binding sites. The wild type protein, with N-terminal sequence (His-4)-X(3)-(Met-8)-X(3)-(His-12), exhibits a classic Cu(2+)-nitrogen spectrum, with g = 2.06 G, g = 2.24 G, and A = 19.3 x 10(-3) cm(-1). No superhyperfine structure is observed. The H4C mutant affords a spectrum that is the combination of two spectra at all stages of saturation. One of the overlapping spectra is nearly identical to the spectrum of wild type, and is due to His ligation. The second spectrum observed yields g = 2.28 and A = 17.1 x 10(-3) cm(-1). The linewidth and tensor values of the second component have been assigned to Cu(2+)-S ligation. In contrast, the H12C mutant exhibits an EPR spectrum at low Cu(2+) occupancy that is very similar to the second set of spectral features observed for H4C, and which is assigned to Cu(2+)-S ligation. No Cu(2+)-His ligation is apparent until the Cu(2+)/N-terminal helices ratio is >1.0. At saturation, the g = 2.00-2.06 region of the spectrum is essentially a mirror image of the spectrum obtained with H4C, and is due to overlapping Cu(2+)-N and Cu(2+)-S EPR spectra. The M8L and M8C mutants were also studied, in order to probe the role of position 8 in the N-terminal helix. Spectral parameters of these mutants are nearly identical to each other and to the wild type spectrum at saturating Cu(2+), suggesting that Met-8 does not act as a direct metal ligand. Together, the results provide the first direct evidence for a binuclear metal ion site between each N-terminal helix pair at the GS-GS interface, with both His-4 and His-12 providing metal ligands.
Polarization-insensitive high-dispersion total internal reflection diffraction gratings (2005)

We report the first experimental realization of total internal reflection (TIR) diffraction gratings. Performance of less than 0.7-dB insertion loss (IL) for both TE and TM polarizations and 0.5-dB polarization-dependent loss (PDL) are predicted over a 50-nm spectral bandwidth with simultaneous fabrication tolerances on the depth and the duty cycle of binary gratings of +/-5% and +/-14%, respectively. Nineteen gratings were fabricated that met these specifications, yielding IL and PDL values less than 0.6 and 0.2 dB, respectively, across the entire 50-nm bandwidth. Measurements made under the Littrow configuration resulted in high efficiency and low PDL across a 100-nm bandwidth, with up to 100% diffraction efficiency within the experimental measurement error.
A tool for integrating conceptual schemas and user views (2003)

An interactive tool has been developed to assist database designers and administrators (DDA) in integrating schemas. It collects the information required for integration from a DDA, performs essential bookkeeping, and integrates schemas according to the semantics provided. The authors present the capabilities of this tool by discussing the integration methodology and the user interface of the tool.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Synthesis and characterization of perovskite PbTiO3 nanoparticles with solution processability (2010)

Ferroelectrics with perovskite structure (ABO3), for example, lead titanate (PbTiO3), are the most studied ferroelectric oxides because of their versatile properties for use in thin film capacitors, electronic transducers, actuators, high-k dielectrics, pyroelectric sensors, and nonlinear optics. The studies on nanostructured PbTiO3 are limited, mainly due to the lack of facile synthetic techniques to prepare nano-sized PbTiO3, i.e., colloidal PbTiO3 nanoparticles. Herein, we presented a novel nonaqueous, relatively low temperature, and highly scalable route to PbTiO3 nanoparticles. The use of oleic acid as surface capping ligand provides nanoparticles with good solubility in organic solvents, thereby enabling the low-cost solution based processing. Rather than the use of expensive bimetallic alkoxide precursors, common chemicals were utilized in this non-hydrolytic thermal decomposition approach; moreover, there is no need for extremely high temperature and long reaction time, making it possible for the large scale synthesis.