gebhart/patrepeval huggingface dataset:
Genetic Diversity of<i>Mycobacterium avium</i>Isolates Recovered from Clinical Samples and from the Environment: Molecular Characterization for Diagnostic Purposes (2008)

Isolation of Mycobacterium avium complex (MAC) organisms from clinical samples may occur in patients without clinical disease, making the interpretation of results difficult. The clinical relevance of MAC isolates from different types of clinical samples (n = 47) from 39 patients in different sections of a hospital was assessed by comparison with environmental isolates (n = 17) from the hospital. Various methods for identification and typing (commercial probes, phenotypic characteristics, PCR for detection of IS1245 and IS901, sequencing of the hsp65 gene, and pulsed-field gel electrophoresis) were evaluated. The same strain was found in all the environmental isolates, 21 out of 23 (91.3%) of the isolates cultured from urine samples, and 5 out of 19 (26.3%) isolates from respiratory specimens. This strain did not cause disease in the patients. Testing best characterized the strain as M. avium subsp. hominissuis, with the unusual feature that 81.4% of these isolates lacked the IS1245 element. Contamination of certain clinical samples with an environmental strain was the most likely event; therefore, characterization of the environmental mycobacteria present in health care facilities should be performed to discard false-positive isolations in nonsterile samples, mainly urine samples. Molecular techniques applied in this study demonstrated their usefulness for this purpose.
Fluorescence pH probe based on microstructured polymer optical fiber (2007)

A kind of optical pH sensor was demonstrated that is based on a pH-sensitive fluorescence dye-doped (eosin) cellulose acetate (CA) thin-film modified microstructured polymer optical fiber (MPOF). It was obtained by directly inhaling an eosin-CA-acetic acid mixed solution into array holes in a MPOF and then removing the solvent (acetic acid). The sensing film showed different fluorescence intensities to different pH solutions in a pH range of 2.5-4.5. Furthermore, the pH response range could be tailored through doping a surfactant, hexadecyl trimethyl ammonium bromide (CTAB), in the sensing film.
Dynamic logic CMOS circuits (1984)

A structure of dynamic CMOS logic based on the direct interconnection of p-channel logic and n-channel logic dynamic gates is reported. Prevention of glitches and other circuit problems are discussed. Application to a 16-bit parallel-adder design resulted in improved speed as well as important savings in layout area when compared to standard static design.
HUMAN ERYTHROCYTE BUT NOT BRAIN ACETYLCHOLINESTERASE HYDROLYSES HEROIN TO MORPHINE (1999)

1. In human blood, heroin is rapidly hydrolysed by sequential deacylation of two ester bonds to yield first 6-monoacetylmorphine (6-MAM), then morphine. 2. Serum butyrylcholinesterase (BuChE) hydrolyses heroin to 6-MAM with a catalytic efficiency of 4.5/min per mumol/L, but does not proceed to produce morphine. 3. In vitro, human erythrocyte acetylcholinesterase (AChE) hydrolyses heroin to 6-MAM, with a catalytic efficiency of 0.5/min per mumol/L under first-order kinetics. Moreover, erythrocyte AChE, but not BuChE is capable of further hydrolysing 6-MAM to morphine, albeit at a considerably slower rate. 4. Both hydrolysis steps by erythrocyte AChE were totally blocked by the selective AChE inhibitor BW284c51 but were not blocked by the BuChE-specific inhibitor, iso-OMPA (tetraisopropylpyrophosphoramide). 5. The brain synaptic form of AChE, which differs from the erythrocyte enzyme in its C-terminus, was incapable of hydrolysing heroin. 6. Heroin suppressed substrate hydrolysis by antibody-immobilized erythrocyte but not by brain AChE. 7. These findings reveal a new metabolic role for erythrocyte AChE, the biological function of which is as yet unexplained, and demonstrate distinct biochemical properties for the two AChE variants, which were previously considered catalytically indistinguishable.
Controlling data in the cloud (2009)

Cloud computing is clearly one of today's most enticing technology areas due, at least in part, to its cost-efficiency and flexibility. However, despite the surge in activity and interest, there are significant, persistent concerns about cloud computing that are impeding momentum and will eventually compromise the vision of cloud computing as a new IT procurement model. In this paper, we characterize the problems and their impact on adoption. In addition, and equally importantly, we describe how the combination of existing research thrusts has the potential to alleviate many of the concerns impeding adoption. In particular, we argue that with continued research advances in trusted computing and computation-supporting encryption, life in the cloud can be advantageous from a business intelligence standpoint over the isolated alternative that is more common today.
Methods of improving the performance of drilled piers in weak rock (1983)

Investigations were made of two methods to improve the load–displacement performance of concrete piers socketed into very weak rock. Results of load tests on six full-size piers are reported.One method involved cutting grooves into the socket wall to roughen the pier–rock interface and thus increase the shaft resistance component of load support. Two types of piers were tested: piers with a void at the base (shaft resistance only) and piers having both shaft resistance and end-bearing resistance. In the latter case, flatjack load cells were installed to measure base loads. The test results indicated that increasing the roughness of the socket wall can cause important increases in shaft resistance.The second method involved the application of preload to the socket base to increase the end-bearing component of load support at small displacements. The test results showed that preloading the socket base resulted in a stiffer load–displacement behaviour of the pier–socket system.The initial portions of the load–displacement curves from all the tests were linear, reflecting elastic behaviour of the pier–socket system. This elastic behaviour did not appear to rely on socket roughness. Beyond the limit of proportionality, the load–displacement behaviour of each pier departed from that of an elastic system, and this departure was more rapid for the piers with smooth sockets than for those with roughened socket walls. Preloading the socket base caused the elastic range of loading to be extended.Two design approaches, limit-state analysis and elastic-state analysis, are discussed. Keywords: drilled piers and caissons, large-bored piles, shaft resistance, grooved shaft, load transfer, preload, shale, weak rock.
Development of Plant Prime-Editing Systems for Precise Genome Editing (2020)

Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an HPT<sup>-ATG</sup> reporter in rice. Our results showed that the pPE2 system could induce programmable editing at different genome sites. In transgenic T<sub>0</sub> plants, pPE2-generated mutants occurred with 0%-31.3% frequency, suggesting that the efficiency of pPE2 varied greatly at different genomic sites and with prime-editing guide RNAs of diverse structures. To optimize editing efficiency, guide RNAs were introduced into the pPE2 system following the PE3 and PE3b strategy in human cells. However, at the genomic sites tested in this study, pPE3 systems generated only comparable or even lower editing frequencies. Furthemore, we developed a surrogate pPE2 system by incorporating the HPT<sup>-ATG</sup> reporter to enrich the prime-edited cells. The nucleotide editing was easily detected in the resistant calli transformed with the surrogate pPE2 system, presumably due to the enhanced screening efficiency of edited cells. Taken together, our results indicate that plant prime-editing systems we developed could provide versatile and flexible editing in rice genome.
Hand gestures to control infotainment equipment in cars (2014)

Controlling multiple devices while driving steals drivers' attention from the road and is becoming the cause of accidents in 1 out of 3 cases. Many research efforts are being dedicated to design, manufacture and test Human-Machine Interfaces that allow operating car devices without distracting the drivers' attention. A complete system for controlling the infotainment equipment through hand gestures is explained in this paper. The system works with a visible-infrared camera mounted on the ceiling of the car and pointing to the shift-stick area, and is based in a combination of some new and some well-known computer vision algorithms. The system has been tested by 23 volunteers on a car simulator and a real vehicle and the results show that the users slightly prefer this system to an equivalent one based on a touch-screen interface.
Functional tests for arbitration SRAM-type FIFOs (2003)

First-In-First-Out (FIFO) memories are becoming increasingly popular as buffer storage between subsystems operating at different data rates. One way to implement a FIFO is to use a single-port SRAM memory with arbitration logic to resolve conflicts due to simultaneous read and write requests. The well-know functional tests for SRAMs (van de Goor, 1990 and 1991) cannot be applied to FIFOs because of their built in access restrictions. Functional fault models and functional tests for FIFOs are presented before, together with a set of tests and their correctness proofs for arbitration SRAM-type FIFOs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Jasmonate-Based Wound Signal Transduction Requires Activation of WIPK, a Tobacco Mitogen-Activated Protein Kinase (1999)

Shigemi Seo, Hiroshi Sano, Yuko Ohashi, Jasmonate-Based Wound Signal Transduction Requires Activation of WIPK, a Tobacco Mitogen-Activated Protein Kinase, The Plant Cell, Vol. 11, No. 2 (Feb., 1999), pp. 289-298
Low-loss subwavelength plastic fiber for terahertz waveguiding (2006)

We report a simple subwavelength-diameter plastic wire, similar to an optical fiber, for guiding a terahertz wave with a low attenuation constant. With a large wavelength-to-fiber-core ratio, the fractional power delivered inside the lossy core is reduced, thus lowering the effective fiber attenuation constant. In our experiment we adopt a polyethylene fiber with a 200 microm diameter for guiding terahertz waves in the frequency range near 0.3 THz in which the attenuation constant is reduced to of the order of or less than 0.01 cm(-1). Direct free-space coupling efficiency as high as 20% can be achieved by use of an off-axis parabolic mirror. Furthermore, all the plastic wires are readily available, with no need for complex or expensive fabrication.
Role of Immune Response in Nonalcoholic Fatty Liver Disease (2008)

Nonalcoholic fatty liver disease (NAFLD) is a spectrum of hepatic pathology ranging from nonalcoholic fatty liver (NAFL; steatosis) at the most clinically benign end of the spectrum to cirrhosis at the opposite extreme, where most liver-related morbidity and mortality occur. Nonalcoholic steatohepatitis (NASH) is a lesion of intermediate severity. NASH is characterized by overt hepatocyte injury and death. It is often accompanied by hepatic infiltration with inflammatory cells (1). Some individuals with NASH gradually accumulate hepatic fibrosis, and eventually develop cirrhosis (2). Over time, hepatocellular carcinomas emerge in a small proportion of individuals with NAFLD-related cirrhosis (3). The pathogenesis of NAFLD is the subject of much research because NAFLD is one of the most common causes of chronic liver disease, particularly in countries such as the United States that are in the midst of an obesity epidemic (4,(5)).
A Simplified Format for the Model Elimination Theorem-Proving Procedure (1969)

article Free AccessA Simplified Format for the Model Elimination Theorem-Proving Procedure Author: D. W. Loveland Carnegie-Mellon University, Pittsburgh, Pennsylvania Carnegie-Mellon University, Pittsburgh, PennsylvaniaView Profile Authors Info & Claims Journal of the ACMVolume 16Issue 3pp 349–363https://doi.org/10.1145/321526.321527Published:01 July 1969Publication History 112citation494DownloadsMetricsTotal Citations112Total Downloads494Last 12 Months26Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Antitumor Activity of Prosopis glandulosa Torr. on Ehrlich Ascites Carcinoma (EAC) Tumor Bearing Mice. (2011)

The antitumor activity of ethanol extract of Prosopis glandulosa Torr. (EPG) was evaluated against Ehrlich ascites carcinoma (EAC) tumor model in Swiss albino mice on dose dependent manner. The activity was assessed using survival time, average increase in body weight, hematological parameters and solid tumor volume. Oral administration of EPG at the dose of 100, 200 and 400 mg/Kg, significantly (p < 0.001) increased the survival time and decreased the average body weight of the tumor bearing mice. After 14 days of inoculation, EPG was able to reverse the changes in the hematological parameters, protein and PCV consequent to tumor inoculation. Oral administration of EPG was effective in reducing solid tumor mass development induced by EAC cells. The results indicate that EPG possess significant antitumor activity on dose dependent manner.
Xanthohumol, a prenylated chalcone from hops, modulates hepatic expression of genes involved in thyroid hormone distribution and metabolism (2010)

In the present study, we analyzed the influence of xanthohumol (XN) on thyroid hormone (TH) distribution and metabolism in rats. A potent and selective competition of XN for thyroxine (T4) binding to transthyretin (IC(50)=1 microM at 1.7 nM [(125)I]T4) was found in human and rat sera in vitro. Female rats treated orally with XN showed increased hepatic expression of T4-binding globulin and decreased transthyretin and albumin. Thyrotropin levels and hepatic type 1 deiodinase activity were moderately increased. Northern blot analysis revealed diminished expression of liver sulfotransferase (Sult1a1) and uridine-diphosphate glucuronosyltransferase (Ugt1a1) after XN treatment. The transcript levels of constitutive androstane receptor (CAR), known to be involved in regulation of enzymes metabolizing hormones, drugs and xenobiotics, was lower in rats treated with >10 mg XN/kg body weight per day. Immunoblot analysis indicates reduced amounts of CAR protein. The phenobarbital-inducible cytochrome P450 mRNA level was decreased in rats treated with >10 mg XN/kg/day, in agreement with reduced CAR protein. Although only moderate changes in TH serum levels were observed, the XN-dependent altered expression of components involved in TH homeostasis might be important not only for hormone metabolism, but also for hepatic phase I and II elimination of drug metabolites and xenobiotics.
Using Monte Carlo simulations to commission photon beam output factors—a feasibility study (2003)

This study investigates the feasibility of using Monte Carlo methods to assist the commissioning of photon beam output factors from a medical accelerator. The Monte Carlo code, BEAMnrc, was used to model 6 MV and 18 MV photon beams from a Varian linear accelerator. When excellent agreements were obtained between the Monte Carlo simulated and measured dose distributions in a water phantom, the entire geometry including the accelerator head and the water phantom was simulated to calculate the relative output factors. Simulated output factors were compared with measured data, which consist of a typical commission dataset for the output factors. The measurements were done using an ionization chamber in a water phantom at a depth of 10 cm with a source-detector distance of 100 cm. Square fields and rectangular fields with widths and lengths ranging from 4 cm to 40 cm were studied. The result shows a very good agreement (< 1.5%) between the Monte Carlo calculated and the measured relative output factors for a typical commissioning dataset. The Monte Carlo calculated backscatter factors to the beam monitor chamber agree well with measured data in the literature. Monte Carlo simulations have also been shown to be able to accurately predict the collimator exchange effect and its component for rectangular fields. The information obtained is also useful to develop an algorithm for accurate beam modelling. This investigation indicates that Monte Carlo methods can be used to assist commissioning of output factors for photon beams.
Untersuchung von O‐, S‐ und N‐vinylverbindungen. IV. Darstellung und polymerisation von formaldehyd‐S‐vinylmercaptalen (1966)

Abstract Formaldehyd‐S‐vinylmercaptale wurden durch Dehydratisieren aus den entsprechenden β‐Hydroxyäthylmercaptalen dargestellt. Die Monomeren konnten radikalisch (mit AIBN) und kationisch (mit BF 3 ‐ätherat) polymerisiert werden. Die für die radikalische Polymerisation ermittelten Polymerisationsgrade (P̄ ∼ 100) sind durch die Reaktionsfähigkeit der ‐S‐CH 2 ‐S‐Gruppe gegenüber Radikalen von der Initiatorkonzentration weitgehend unabhängig. Acylperoxide konnten als Initiatoren nicht verwendet werden, da die Mercaptalgruppe einen induzierten Peroxidzerfall bedingt. Die kationische Polymerisation gab über Sulfoniumsalze oligomere Produkte.
Reversal of spatial memory impairments in aged rats by nerve growth factor and neurotrophins 3 and 4/5 but not by brain-derived neurotrophic factor. (1994)

Aged rats, displaying impairments in spatial learning and memory associated with marked cellular atrophy of forebrain cholinergic neurons, received intracerebroventricular infusions of one of the four neurotrophins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3), or neurotrophin 4/5 (NT-4/5), or a combination of NGF and BDNF, or vehicle. During the 4-week infusion period rats receiving NGF, NT-3, or NT-4/5 showed improved acquisition and retention of spatial memory. With NGF and NT-3, but not NT-4/5, this was accompanied by a significant reduction in cholinergic neuron atrophy in septum, nucleus basalis, and striatum. BDNF, in contrast, was without effect either alone or in combination with NGF. These results show that memory deficits associated with aging can be reversed by several members of the neurotrophin family and that this effect may be mediated through activation of multiple neurotrophin receptors associated with cholinergic and possibly noncholinergic systems in the brain.
[Specific binding of human bone morphogenetic protein (2A) with mouse osteoblastic cells]. (1996)

Human bone morphogenetic protein 2A (hBMP2A) cDNA terminal S67 nucleotides were cloned and expressed in a phage display vector pCSM21. Human BMP2A C-terminal peptide displayed on the surface of the phage can bind specifically to the surface of mouse osteoblastic cell (MC3T3) membrane. ELISA assay showed a positive signal of the binding by using antibody against M13 phage gene 8 protein. After labeling with 3HTdR, the counts of the binding groups were 3 to 10 times higher than the control groups. It suggests that the surface of MC3T3 cells exist the receptor for hBMP2A.
Fault-tolerant clock synchronization for distributed systems using continuous synchronization messages (2002)

We present a probabilistic synchronization algorithm which sends periodic synchronization messages, instead of periodic bursts of synchronization messages as other algorithms do. Our "continuous" approach therefore avoids the burst network loads of other algorithms. Nodes always have current estimates of other nodes' clocks, allowing them to monitor the state of system synchronization, and adjust their clocks as needed. The algorithm is fault-tolerant, and may be easily adapted to a wide variety of systems and networks. We analyze and simulate the algorithm's performance on a 64-node hypercube, and show that the algorithm provides tight synchronization while imposing only a light load on the network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Strong Coupling between Localized Plasmons and Organic Excitons in Metal Nanovoids (2006)

Hybrid emitting exciton-plasmonic composites are constructed by coating arrays of spherical nanovoids embedded in a gold film with organic semiconducting molecular J-aggregate films. In such plasmonic crystals, localized plasmons confined inside the voids can be excited. We report the first observation of polaritonic spectral narrowing and strong coupling between localized plasmons and J-aggregate excitons with Rabi splittings of 230 meV at room temperature.
Relations between <i>in vitro</i> cytotoxicity and crosslinked dermal sheep collagens (1992)

Collagen-based biomaterials have found various applications in the biomedical field. However, collagen-based biomaterials may induce cytotoxic effects. This study evaluated possible cytotoxic effects of (crosslinked) dermal sheep collagen (DSC) using a 7-d-methylcellulose cell culture with human skin fibroblasts. Non-crosslinked DSC (NDSC), hexamethylene-diisocyanate-crosslinked DSC (HDSC), and glutaraldehyde-crosslinked DSC (GDSC), their extracts (1 x 10 d to 4 x 10 d extracts), or the corresponding extracted DSC samples were tested. Cell growth was evaluated by cell counting, while cell morphology was assessed by light microscopy and transmission-electron microscopy. Both GDSC and, to a lesser extent, HDSC, induced cytotoxicity, observed as inhibited cell growth and deviant cell morphology. The deviant morphology consisted of extensive accumulations of lipid, reduction in the amount and dilatation of rough endoplasmatic reticulum, increased inclusions of cell remnants, and relatively rounded cell membranes. With HDSC, both primary cytotoxicity, due to extractable products from the material, and secondary cytotoxicity, possibly due to a release of cytotoxic products resulting from enzymatic cell-biomaterial interactions, could be discriminated. With GDSC, however, no clear distinction between primary and secondary cytotoxicity could be made. With NDSC, only primary cytotoxicity, measured as low inhibition of cell proliferation, but without deviant morphology, was observed. These remarkable differences in cytotoxicity are discussed in relation to residual agents and specific crosslinks present in DSCs as a consequence of processing and the crosslinking agents used. The residual agents and the specific crosslinks give rise to differences in direct release of products and in sensitivity to hydrolysis and enzymatic breakdown.
Review—Electromobility: Batteries or Fuel Cells? (2015)

This study provides an analysis of the technological barriers for all-electric vehicles, either based on batteries (BEVs) or on H2-powered proton exchange membrane (PEM) fuel cells (FCEVs). After an initial comparison of the two technologies, we examine the likely limits for lithium ion batteries for BEV applications, and compare the projected cell- and system-level energy densities with those which could be expected from lithium-air and lithium-sulfur batteries. Subsequently, we will review the current development status of H2 PEM fuel cells, with particular attention to their viability with regards to the required amount of platinum and the resulting cost and availability constraints.