gebhart/patrepeval huggingface dataset:
Susceptibility of Nrf2-Null Mice to Steatohepatitis and Cirrhosis upon Consumption of a High-Fat Diet Is Associated with Oxidative Stress, Perturbation of the Unfolded Protein Response, and Disturbance in the Expression of Metabolic Enzymes but Not with Insulin Resistance (2014)

Mice lacking the transcription factor NF-E2 p45-related factor 2 (Nrf2) develop more severe nonalcoholic steatohepatitis (NASH), with cirrhosis, than wild-type (Nrf2(+/+)) mice when fed a high-fat (HF) diet for 24 weeks. Although NASH is usually associated with insulin resistance, HF-fed Nrf2(-/-) mice exhibited better insulin sensitivity than HF-fed Nrf2(+/+) mice. In livers of HF-fed mice, loss of Nrf2 resulted in greater induction of lipogenic genes, lower expression of β-oxidation genes, greater reduction in AMP-activated protein kinase (AMPK) levels, and diminished acetyl coenzyme A (CoA) carboxylase phosphorylation than in the wild-type livers, which is consistent with greater fatty acid (FA) synthesis in Nrf2(-/-) livers. Moreover, primary Nrf2(-/-) hepatocytes displayed lower glucose and FA oxidation than Nrf2(+/+) hepatocytes, with FA oxidation partially rescued by treatment with AMPK activators. The unfolded protein response (UPR) was perturbed in control regular-chow (RC)-fed Nrf2(-/-) mouse livers, and this was associated with constitutive activation of NF-κB and JNK, along with upregulation of inflammatory genes. The HF diet elicited an antioxidant response in Nrf2(+/+) livers, and as this was compromised in Nrf2(-/-) livers, they suffered oxidative stress. Therefore, Nrf2 protects against NASH by suppressing lipogenesis, supporting mitochondrial function, increasing the threshold for the UPR and inflammation, and enabling adaptation to HF-diet-induced oxidative stress.
Optically Transparent, Ultrathin Pt Films as Versatile Metal Substrates for Molecular Optoelectronics (2006)

Abstract This contribution reports a simple, straightforward method (cool sputtering) of fabricating robust, homogeneous, conductive, and optically transparent ultrathin Pt films. Their morphological, structural, mechanical, electrical, and optical properties are reported. The morphology and structure of these Pt films are investigated by scanning electron microscopy, transmission electron microscopy, atomic force microscopy, and X‐ray diffraction. The ultrathin Pt films, approximately 20 nm thick, are characterized by a homogenous, polycrystalline structure, with a tendency to adopt a (111) texture upon the thermal treatment. Moreover, thermal treatment (annealing or flaming) of the as‐prepared films also substantially improves their chemical and mechanical robustness. F films behave as bulk Pt in terms of electrical resistivity and suitability as working electrodes in cyclic voltammetry experiments. Overall, the unique combination of these excellent features: homogeneity, robustness, and conductivity, in addition to the high optical transparency in the 300–800 nm range of the electromagnetic spectrum, make ultrathin Pt films appropriate for a variety of applications in the field of molecular optoelectronics. The formation of functional molecular self‐assembled monolayers (SAMs) on these transparent, conductive films allows their optical monitoring using transmission optical spectroscopy, as well as the probing of their electrical properties. The potential of such Pt films as suitable metal substrates in opto‐ and nanoelectronics is proven by representative applications, including switching of prototypical photochromic and electrochromic species in SAMs and molecule–metal junctions.
Fluorescent Probes of the Molecular Environment within Mesostructured Silica/Surfactant Composites under High Pressure (2000)

The molecular environment of the organic component of periodic silica/surfactant composites under elevated pressure is studied using a rigidochromic Re complex as a molecular probe. This amphiphilic organometallic complex is incorporated into the organic component of the silica/surfactant composite during synthesis. Shifts in the luminescence peak position of the Re complex indicate changes in the rigidity or viscosity of the surrounding matrix. In the experiment, under elevated pressure, the luminescence from the complex provides a measure of the changing molecular environment inside the mesophase. The results confirm conclusions from earlier experiments on the mechanical properties of silica/surfactant composites under high pressure: Although an increase in viscosity is observed with increasing pressure, the molecular environment does not become fully rigid until pressures as high as 10 GPa have been reached. This pressure is significantly higher than the solidification point of most pure organic phases and suggests that the mechanical strength of the silica framework provides a protected molecular environment for organic molecules situated within the silica nanopores.
Immune Modulation by Adjuvants Combined with Diphtheria Toxoid Administered Topically in BALB/c Mice After Microneedle Array Pretreatment (2009)

The potency and the quality of the immune response against DT administered by microneedle array mediated TCI can be modulated by co-administration of adjuvants.
Plans and Situated Actions: The Problem of Human-Machine Communication (1990)

Preface Acknowledgements 1. Introduction 2. Interactive artefacts 3. Plans 4. Situated actions 5. Communicative resources 6. Case and methods 7. Human-machine communication 8. Conclusion References Indices.
Comparison of the Formation of 8-Hydroxy-2‘-deoxyguanosine and Single- and Double-Strand Breaks in DNA Mediated by Fenton Reactions (1998)

The formation of 8-hydroxydeoxyguanosine (8-OHdG) and both single- and double-strand breaks in DNA by Fenton-type reactions has been investigated. Salmon sperm DNA was exposed to hydrogen peroxide (50 mM) and one of nine different transition-metal ions (25 microM-1 mM). Modified DNA was isolated and subjected to analysis by liquid chromatography coupled to an electrochemical detection system (LC-ECD), to evaluate the formation of 8-OHdG. The highest yield of 8-OHdG was obtained following treatment of DNA with the chromium(III) Fenton reaction (a maximum of 19 400/10(6) nucleotides), followed by iron(II) (13 600), vanadium(III) (5800), and copper(II) (5200). The chromium(VI) Fenton reaction generated a moderate yield of 8-OHdG (3600/10(6) nucleotides), while the yield obtained in DNA treated with cobalt(II), nickel(II), cadmium(II), and zinc Fenton reactions was not significantly higher than in control incubations of DNA with hydrogen peroxide alone. Similar treatment of the double-stranded plasmid pBluescript K+ with hydrogen peroxide (1 mM) and each transition-metal ion (1-100 microM) followed by quantitative agarose gel electrophoresis demonstrated that open-circle DNA, resulting from single-strand breaks, was generated in Fenton reactions involving all nine metal ions. In contrast, linear DNA was only formed in Fenton reactions involving chromium(III), copper(II), iron(II), and vanadium(III) ions. Formation of linear DNA, under conditions that generated relatively few single-strand breaks, suggests that these four transition-metal ions partake in Fenton reactions to generate true double-strand breaks. Furthermore, the generation of 8-OHdG exhibits a good correlation with the formation of double-strand breaks, suggesting that they arise by a similar mechanism.
Factors Predictive of Tumor-Positive Nonsentinel Lymph Nodes After Tumor-Positive Sentinel Lymph Node Dissection for Melanoma (2004)

Thicker primary and larger SN tumor size are factors that correlate best with tumor-positive NSN. Although none of these factors are absolutely predictive of residual nodal disease, these factors must be strongly considered if the SN contains metastasis, as they provide enhanced risk assessment for NSN tumor-positivity.
Design and analysis of a new parallel grasper having spherical motion (2005)

A new parallel grasper mechanism having 3-DOF spherical motions is proposed. The mechanism consists of one parallel four-bar chain and four serial sub-chains that are connected in parallel to the four-bar chain. Particularly, all joint axes of the mechanism are placed to have a common intersection point securing that all joint motion spaces of the mechanism are confined within the 3-DOF spherical motion spaces. Thus, the mechanism can not only grasp irregular shaped objects by changing configuration of its four-bar chain, but also exhibit 3 DOF spherical motions after grasping. The position analysis and kinematic analysis of the mechanism are performed. Also, its kinematic characteristics are investigated through simulation in aspects of three kinematic design indices. Lastly, a prototype was developed and tested through motion tracking and grasping experiments.
A direct radioimmunoassay for aldosterone in plasma. (1983)

This rapid radioimmunoassay for aldosterone is performed directly on 100 microL of unprocessed plasma, with 125I-labeled aldosterone as the labeled antigen. Our use of steroid-free plasma in preparing the standard curve resulted in an overestimate of aldosterone; this problem was overcome by adding to such plasma a mixture of other steroids to provide a constant steroid/aldosterone ratio. Over a wide range of aldosterone concentrations, results agreed well between the present assay and a routine method involving solvent extraction and paper chromatography (r = 0.85), and sensitivity (20 ng/L) and inter- (10.4%) and intra- (3.9%) assay CVs were better with the present assay. Our assay is especially useful for multiple samples and (or) when only small-volume samples are available.
Further breeding records from Zambia (No. 7) (1969)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
Rapid identification of elaiophylin and geldanamycin inStreptomyces fermentation broths using CPC coupled with a photodiode array detector and LC-MS methodologies (1995)

During the course of screening microbial broth extracts in various high through-put bioassays (eg receptor binding or enzyme inhibition), several actinomycete cultures were discovered to produce active metabolites. The natural products elaiophylin and/or geldanamycin are produced by several Streptomyces violaceusniger strains, and the bioactivity of the extracts from these cultures was frequently associated with the fractions containing these metabolites. CPC coupled to a photodiode array detector and LC-MS techniques were applied to these broth extracts to ascertain rapidly when these natural products were present. These methodologies allowed us to identify the metabolites quickly in the crude extract, and the application demonstrated further the utility of CPC-photodiode array detection and LC-MS as powerful, initial analytical tools in analyses of the complex metabolite profiles produced by microorganisms.
Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by α5 subunit-containing γ-aminobutyric acid type A receptors (2004)

The principal inhibitory neurotransmitter in the mammalian brain, gamma-aminobutyric acid (GABA), is thought to regulate memory processes by activating transient inhibitory postsynaptic currents. Here we describe a nonsynaptic, tonic form of inhibition in mouse CA1 pyramidal neurons that is generated by a distinct subpopulation of GABA type A receptors (GABA(A)Rs). This tonic inhibitory conductance is predominantly mediated by alpha5 subunit-containing GABA(A)Rs (alpha5GABA(A)Rs) that have different pharmacological and kinetic properties compared to postsynaptic receptors. GABA(A)Rs that mediate the tonic conductance are well suited to detect low, persistent, ambient concentrations of GABA in the extracellular space because they are highly sensitive to GABA and desensitize slowly. Moreover, the tonic current is highly sensitive to enhancement by amnestic drugs. Given the restricted expression of alpha5GABA(A)Rs to the hippocampus and the association between reduced alpha5GABA(A)R function and improved memory performance in behavioral studies, our results suggest that tonic inhibition mediated by alpha5GABA(A)Rs in hippocampal pyramidal neurons plays a key role in cognitive processes.
Hydroxynitrile Lyases: Biological Sources and Application as Biocatalysts (2004)

We review the state of the art regarding the application of hydroxynitrile lyases to obtain, enantioselectively, (R)- and (S)-cyanohydrins of aldehydes and ketones. Special emphasis is given to recent preparative applications and to research for extending the number of plants serving as sources for the enzyme. Depending on the plant family, the mechanism of the enzyme-catalysed reaction can be different. A novel area of research is the consideration of evolutionary aspects on the basis of structure comparisons.
3‐Nitro‐2‐pyridinesulfenates as Efficient Solution‐ and Solid‐Phase Disulfide Bond Forming Agents (2017)

In this paper, a new disulfide-forming agent based on the finding that alkoxy 3-nitro-2-pyridinesulfenates (Npys-OR) can oxidize thiol groups is reported. Methyl 3-nitro-2-pyridinesulfenate (Npys-OMe), which is easily prepared from 3-nitro-2-pyridinesulfenyl chloride in a one-step reaction and has a reduction peak potential (E<sub>pc</sub> ) of -0.541 V versus Ag/AgCl, produces the cyclic nonapeptide oxytocin from its linear form in good yield (92 %) with minimal oligomer formation. Npys-OMe in the solid phase also demonstrated excellent results in oxytocin synthesis. Other disulfide-containing peptides, such as α-human atrial natriuretic peptide and α-conotoxin ImI, were also successfully synthesized. During these syntheses, no side reactions of methionine (Met) and tryptophan (Trp) residues or the S-acetamidomethyl (Acm) protecting group were detected. These results suggested that Npys-OMe or its solid-phase analog provides a new strategy for regioselective disulfide bond formation to assist the synthesis of complex disulfide-rich peptides.
Novel heterometal-organic complexes as first single source precursors for up-converting NaY(Ln)F<sub>4</sub>(Ln = Yb, Er, Tm) nanomaterials (2011)

First heterometal-organic single source precursors for NaYF(4) nanomaterials as a host matrix for up-conversion emission are reported. These novel heterobimetallic derivatives NaY(TFA)(4)(diglyme) (1), [Na(triglyme)(2)][Y(2)(TFA)(7)(THF)(2)] (2) and Na(2)Y(TFA)(5)(tetraglyme) (3) (TFA = trifluoroacetate), which were fully characterized by elemental analysis, FT-IR and (1)H NMR spectroscopy, TG-DTA data as well as single crystal X-ray structures, are advantageous in terms of being anhydrous and having lower decomposition temperatures in comparison to the homometallic precursor Y(TFA)(3)(H(2)O)(3). In addition, they also contain chelating glyme ligands, which act as capping reagents during decomposition to control the NaYF(4) particle size and render them monodisperse in organic solvents. On decomposition in 1-octadecene, the molecular derivatives 1 and 3 are converted, in the absence of any surfactant or capping reagent, to cubic NaYF(4) nanocrystals at significantly lower temperatures (below 250 °C). At higher temperature, a mixture of the cubic and hexagonal phases was obtained, the relative ratio of the two phases depending on the reaction temperature. A pure hexagonal phase, which is many folds more efficient for UC emission than the cubic phase, was obtained by calcining nanocrystals of mixed phase at 400 °C. In order to co-dope this host matrix with up-converting lanthanide cations, analogous complexes NaLn(TFA)(4)(diglyme) [Ln = Er (4), Tm (5), Yb (6)] and Na(2)Ln(TFA)(5)(tetraglyme) [Ln = Er (7), Yb (8)] were also prepared and characterized. The decomposition in 1-octadecene of suitable combinations and appropriate molar ratios of these yttrium, ytterbium and erbium/thulium derivatives gave cubic and/or hexagonal NaYF(4): Yb(3+), Er(3+)/Tm(3+) nanocrystals (NCs) capped by diglyme or tetraglyme ligands, which were characterized by IR, TG-DTA data, EDX analysis and TEM studies. Surface modification of these NCs by ligand exchange reactions with poly acrylic acid (PAA) and polyethyleneglycol (PEG) diacid 600 was also carried out to render them water soluble. The THF solutions of suitable combinations of the diglyme derivatives were also used to elaborate the thin films of NaYF(4):Yb(3+), Er(3+)/Tm(3+) on a glass or Si wafer substrate by spin coating. The multicolour up-conversion fluorescence was successfully realized in the Yb(3+)/Er(3+) (green/red) and Yb(3+)/Tm(3+) (blue/violet) co-doped NaYF(4) nanoparticles and thin films, which demonstrates that they are promising UC nanophosphors of immense practical interest. The up-conversion excitation pathways for the Er(3+)/Yb(3+) and Tm(3+)/Yb(3+) co-doped materials are discussed.
TGF-β3: A potential biological therapy for enhancing chondrogenesis (2009)

There is robust in vitro evidence suggesting a positive dose- and time-dependent effect of TGF-beta3 on anabolic chondrogenic gene markers such as alpha1-collagen type II and cartilage oligomeric matrix protein in human mesenchymal stem cells. TGF-beta3 cultured with silk elastin-like polymer scaffold carrier exhibits significantly increased glycosaminoglycan and collagen content. In vivo data showed that TGF-beta3 cultured with ovine mesenchymal stem cells in a chitosan scaffold stimulated the growth of hyaline cartilage that was fully integrated into host cartilage tissue of sheep. We highlight the potential for the clinical enhancement of cartilage formation through the use of TGF-beta3 with a suitable dose and scaffold carrier.
Applying Immittance Spectroscopy to Monitoring Hydrogen Peroxide in the Presence of Ascorbic Acid. Part I: Theoretical Considerations (2001)

The theoretical prospect of utilizing AC immittance characterization of an electrochemical system to tailor the response for the selective amperometric monitoring of a kinetically less-favored analyte in the presence of a kinetically more-favored species is explored. The selective measurement of hydrogen peroxide in the presence of ascorbic acid is considered as a model case and an AC immittance model is developed from a first-principles approach based upon the partial differential equations governing transport and the kinetics of the electron transfers. The influence of various system parameters on the AC immittance spectra are investigated to identify the measurement conditions whereby the AC signal from hydrogen peroxide oxidation will be significantly larger than the AC signal from ascorbic acid.
Myeloproliferative neoplasms can be initiated from a single hematopoietic stem cell expressing <i>JAK2</i> -V617F (2014)

The majority of patients with myeloproliferative neoplasms (MPNs) carry a somatic JAK2-V617F mutation. Because additional mutations can precede JAK2-V617F, it is questioned whether JAK2-V617F alone can initiate MPN. Several mouse models have demonstrated that JAK2-V617F can cause MPN; however, in all these models disease was polyclonal. Conversely, cancer initiates at the single cell level, but attempts to recapitulate single-cell disease initiation in mice have thus far failed. We demonstrate by limiting dilution and single-cell transplantations that MPN disease, manifesting either as erythrocytosis or thrombocytosis, can be initiated clonally from a single cell carrying JAK2-V617F. However, only a subset of mice reconstituted from single hematopoietic stem cells (HSCs) displayed MPN phenotype. Expression of JAK2-V617F in HSCs promoted cell division and increased DNA damage. Higher JAK2-V617F expression correlated with a short-term HSC signature and increased myeloid bias in single-cell gene expression analyses. Lower JAK2-V617F expression in progenitor and stem cells was associated with the capacity to stably engraft in secondary recipients. Furthermore, long-term repopulating capacity was also present in a compartment with intermediate expression levels of lineage markers. Our studies demonstrate that MPN can be initiated from a single HSC and illustrate that JAK2-V617F has complex effects on HSC biology.
Real-time Linear Projection Speed Sign Detection System in Low Light Conditions (2007)

In this paper, a real-time algorithm for detecting speed signs in low light conditions is presented. This application oriented algorithm starts with performing the popular difference of Gaussian (DoG) filter as a pre-processing step. After that pixels are thresholded and classified into predefined classes. The paper introduce an efficient voting method called linear projection, in which each pixel that belongs to any predefined classes continuously votes on an incremental image along its gradient vector to identify centre of the speed sign candidates. This algorithm is fast, reliable, suitable for real-time hardware (FPGA) implementation. A Matlab simulation model is built to verify the algorithm's operation in nighttime driving conditions. A FPGA initial prototype of this algorithm has been implemented and showed promising results.
Asymmetric Conjugate Addition to α‐Halo Enones: Dramatic Effect of Styrene on the Enantioselectivity (2006)

A simple trick could prove generally valuable for asymmetric copper-catalyzed conjugate addition reactions. It was found that the enantioselectivities of such reactions of dialkyl zinc reagents with α-halo enones in the presence of chiral phosphorimidite ligands were dramatically improved upon the addition of styrene, which acts as a radical scavenger to suppress the competitive non-asymmetric radical pathway (see example).
Location- And Time-Dependent Vpd For Privacy-Preserving Wireless Accesses To Cloud Services (2018)

The advent of smartphones in recent years has changed the wireless landscape. Smartphones have become a platform for online user interface to cloud databases. Cloud databases may provide a large set of userprivate and sensitive data (i.e., objects), while smartphone users (i.e., subjects) provide location-sensitive information. Secure and private services in wireless accessing to cloud databases have been discussed actively for the past recent years. However, the previous techniques are unsatisfactory for dynamism of moving subjects’ wireless accesses. In this paper, we propose a novel technique to dynamically generate virtual private databases (VPD) for each access by taking subjects’ location and time information into account. The contribution of this paper includes a privacy-preserving access control mechanism for dynamism of wireless access.
[Factors affecting upper airway patency]. (1990)

We studied the following functional factors which affect upper airway patency. First, the effects of inspiratory resistive loading, increased pulmonary resistance, hypercapnic gas or hypoxic gas loading on the upper airway pressure-flow (P-F) relationship were studied. The upper airway P-F curves under these loading conditions shifted upward with load dependency. Secondly, effects of limb muscle contraction on upper airway resistance (Rua) were studied. Rua decreased reflexly during limb muscle contraction. Thirdly, effects of body position on the stability of the upper airway were studied. The upper airway was kept patent in the lateral position. Fourthly, effects of electrical stimulation of the genioglossus muscle on P-F relationship of upper airway were studied. P-F curves of upper airway shifted upward with increase in stimulation frequency. Fifthly, effects of nasal or oropharyngeal lubrication by artificial surfactant on the critical stimulation frequency (CSF) for upper airway opening were studied. Nasal or oropharyngeal lubrication decreased CSF. Based on these fundamental studies, we applied constrained lateral position, submental stimulation due to demand-type stimulator and nasal or oropharyngeal lubrication for treatment of patients with obstructive sleep apnea syndrome (OSAS). We found these three methods were effective in treatment of OSAS patients.