gebhart/patrepeval huggingface dataset:
Is there a human [psi]? (1996)

The yeast Sup35p protein which is responsible for the [psi] phenotype, is a GTP-binding protein involved in translation termination. It was suggested recently that the [psi] determinant has prion-like properties that were localized in the 114 N-terminal amino acids of the protein. In this study, we show that the 5' end of the human SUP35 gene open reading frame is longer than previously reported by 138 codons. This N-terminal sequence presents similarities with the N-terminus of S. cerevisiae Sup35p protein, involved in [psi] maintenance. By transfection of human cells and Western blotting, we demonstrate that translation is initiated at the first AUG encountered at the 5' end of the human SUP35 gene. The longest form of the protein, which contains the N-terminal extension, is the major form of Sup35p protein in non transfected cells. Moreover, an analog of the long form of Sup35p protein is found in various mouse tissues. We suggest that the protein encoded by SUP35 gene could have, at least in human, the properties described for the yeast [psi] element.
Catalytic Enantioselective Synthesis of Vicinal Dialkyl Arrays (2008)

With a consecutive "asymmetric allylic alkylation (AAA)/cross-metathesis (CM)/conjugate addition (CA)" protocol it is possible to synthesize either stereoisomer of compounds containing a vicinal dialkyl array with excellent stereoselectivity. The versatility of this protocol in natural product synthesis is demonstrated in the preparation of the ant pheromones faranal and lasiol.
Brain Endothelial Cells Control Fertility through Ovarian-Steroid–Dependent Release of Semaphorin 3A (2014)

Neuropilin-1 (Nrp1) guides the development of the nervous and vascular systems, but its role in the mature brain remains to be explored. Here we report that the expression of the 65 kDa isoform of Sema3A, the ligand of Nrp1, by adult vascular endothelial cells, is regulated during the ovarian cycle and promotes axonal sprouting in hypothalamic neurons secreting gonadotropin-releasing hormone (GnRH), the neuropeptide controlling reproduction. Both the inhibition of Sema3A/Nrp1 signaling and the conditional deletion of Nrp1 in GnRH neurons counteract Sema3A-induced axonal sprouting. Furthermore, the localized intracerebral infusion of Nrp1- or Sema3A-neutralizing antibodies in vivo disrupts the ovarian cycle. Finally, the selective neutralization of endothelial-cell Sema3A signaling in adult Sema3aloxP/loxP mice by the intravenous injection of the recombinant TAT-Cre protein alters the amplitude of the preovulatory luteinizing hormone surge, likely by perturbing GnRH release into the hypothalamo-hypophyseal portal system. Our results identify a previously unknown function for 65 kDa Sema3A-Nrp1 signaling in the induction of axonal growth, and raise the possibility that endothelial cells actively participate in synaptic plasticity in specific functional domains of the adult central nervous system, thus controlling key physiological functions such as reproduction.
Reconfigurable frequency with circular polarization for on-body wearable textile antenna (2016)

This paper describes the development of reconfigurable frequency microstrip patch textile antenna. This antenna is composed with a truncated edges rectangular patch incorporated with the slot structure at the ground. Three BAR PIN diodes switches are placed on the slotted ground plane. By controlling the state of the switches, such antenna is able to operate at different operating frequencies. Besides, the antenna has successfully achieve an axial ratio (AR) of less than 3 dB for GPS operating frequency with configurations T2 until T7. The proposed antenna is potential for front end wireless communication system for GPS and WIFI applications.
A performance evaluation study of RPL: Routing Protocol for Low power and Lossy Networks (2010)

In this paper, a performance evaluation of the Routing Protocol for Low power and Lossy Networks (RPL) is presented. Detailed simulations are carried out to produce several routing performance metrics using a set of real-life scenarios. A real outdoor network was reproduced in simulation with the help of topology and link quality data. Behaviors of such a network in presence of RPL, their reason and potential areas of further study/improvement are pointed out.
Clinical assessment of spectral modulation detection for adult cochlear implant recipients: A non-language based measure of performance outcomes (2014)

The results of this study represent the direct clinical translation of a research-proven task of SMD into a quick, clinically feasible format.
Binary Azeotropic Systems - Effect of Temperature on Compositon and Pressure (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBinary Azeotropic Systems - Effect of Temperature on Compositon and PressureJoseph JoffeCite this: Ind. Eng. Chem. 1955, 47, 12, 2533–2535Publication Date (Print):December 1, 1955Publication History Published online1 May 2002Published inissue 1 December 1955https://pubs.acs.org/doi/10.1021/ie50552a047https://doi.org/10.1021/ie50552a047research-articleACS PublicationsRequest reuse permissionsArticle Views296Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Protective effects of pgms on experimental endothelial cells injury (1994)

The protective effects of Propylene Glucol Mannate Sulfate (PGMS), a newly designed mucopolysaccharide extracted from marine alga, on endothelial cells(ES)injured by histamine or NE were evaluated in vivo. The permeability of Evans blue in aortic wall or endothelium-depen-dent vasorelaxation of Ach was investigated. Rats were given PGMS 14mg/100g body weight per day orally for 14 days, the level of Evans blue in aortic wall was lower compared with control animals(P<0.001). Rabbits were given PGMS 75mg/kg body weight perday orally for 7 days, the endothelium dependent vasorelaxation of Ach was more compared with those untreated (P<0.01). The morphological changes of endothelial cell injure induced by NE were slight in animals had received PGMS under the scanning electron microscope. The results showed that PGMS has protective effects on endothelial cell injure induced by histamine or NE in experimental animals.
A new improved coil for tapered-tip catheter for arterial occlusion. (1980)

The original coil emboli were designed for untapered-lip catheters. New coils have been designed for tapered-lip (1mm) catheters and have two helical diameters of 3 and 5 mm, respectively. The adaptation of the new coil to a tapered-tip catheter greatly simplifies its use in arterial occlusion.
Effects of respiratory syncytial virus infection on cough-related airway function and neurotransmitter in guinea pigs (2011)

Objective To investigate the effects of respiratory syncytial virus (RSV) infection on cough-related airway function and neurotransmitter in guinea pigs and to explore the pathogenesis of cough after RSV infection. Methods Sixty SPF male guinea pigs were randomly divided into control group,asthma group and four RSV-infected groups (6,12,28 and 42 d group), ten in each group. Infected animals were inoculated by intranasal instillation of RSV suspension, Plethysmography was used to assess cough reflex sensitivity (CRS) and airway responsiveness (AR). Vanilloid receptor-1 (VR1) mRNA level was detected by real-time PCR. The protein expressions of VR1 and protein gene product-9.5 (PGP-9.5)were measured by immunohistochemistry. Results CRS in 6, 12, 28 and 42 d RSV-infected group [ (8. 00 ± 3. 86), (8. 70± 6.20 ), ( 7.60 ±4.40 ) and ( 6.70 ± 3. 71 ) CCnt] was higher than that in control group [(2. 50±1.43) CCnt,all P ＜0.05] and reached a peak in 12 d RSV-infected group ( P ＜0.01). AR to two high concentrations of methylocholine in 12 d RSV-infected group [( 1 069 ± 156 )%,( 1 846±285) %] was higher than that in control group (all P ＜0.05). VR1 mRNA level in 6,12,28 and 42 d RSV-infected group (1.57 ± 0.43,1.61 ±0. 47 and 1.68 ± 0. 56) was higher than that in control group (1.00±0.34, P ＜0.05), and remained at highest level in 28 d RSV-infected group. Compared with control group, VR1 protein expression in 28 d RSV-infected group and PGP-9.5 in 12 d RSV-infected group increased (all P ＜0. 05). Conclusions The increased CRS with increased AR in a certain degree is the feature of cough caused by RSV. The temporal and spatial variation of VR1 and PGP-9. 5 may be related to the pathogenesis of cough caused by RSV.

Key words: 
Respiratory syncytial virus;  Cough reflex sensitivity;  Airway responsiveness;  Vanilloid receptor;  Protein gene products-9.5
Chemical shrinkage and autogenous shrinkage of Portland cement—metakaolin pastes (1998)

Portland cement (PC)-metakaolin (MK) pastes of compositions 0, 5, 10, 15, 20 and 25% MK and water/binder ratio 0·55 have been subjected to tests for chemical shrinkage, autogenous shrinkage and expansion during soaking. Chemical shrinkage was carried out in 200 ml containers on water-covered 12 mm thick layers of paste, and the volume changes were monitored using 1 ml graduated pipettes. Autogenous shrinkage and expansion were determined by monitoring the dimensional changes of 285 × 25 × 25 mm paste bars, in, respectively, a sealed environment and in water. Volume changes are converted to millilitres per gram of binder for all three systems for purposes of comparison. Chemical shrinkage was found to increase for compositions between 0 and 15% MK and then decrease for compositions above 15% MK. Autogenous shrinkage was found to increase for compositions between 0 and 10% MK and then decrease for compositions above 15% MK. Expansion showed little variation for compositions between 0 and 10% MK and then increased for compositions above 15% MK. These changes are discussed in terms of formation of new hydrate phases and self-desiccation due to hydration.
Positron tomographic assessment of 16 alpha-[18F] fluoro-17 beta-estradiol uptake in metastatic breast carcinoma. (1991)

The positron-emitting estrogenic steroid 16 alpha-[18F]fluoro-17 beta-estradiol (FES) has been shown to exhibit selective uptake in primary breast carcinomas; the uptake of tracer by positron emission tomography (PET) is strongly correlated with the tumor estrogen-receptor concentration. We have now extended the use of this radiopharmaceutical for imaging of metastases of breast carcinoma by PET in 16 patients with clinical or radiographic evidence of metastatic disease. Increased uptake of FES was identified on PET images in 53 of 57 metastatic lesions (93%); only two apparent false-positive foci of FES uptake were seen. In seven of the patients, evaluable PET studies were obtained both before and after initiation of antiestrogen therapy. In all cases, there was a decrease in FES uptake in the tumor deposits after initiation of antiestrogen therapy, and the mean (+/- standard deviation) uptake decreased from 2.22 (+/- 1.23) to 0.80 (+/- 0.42) x 10(3)+ dose/ml. These results indicate that PET with FES has high sensitivity and specificity for detecting metastatic breast carcinoma and provide additional confirmatory evidence that the tumor uptake of this ligand is a receptor-mediated process.
Peptide nucleic acid clamp PCR: A novel K‐<i>ras</i> mutation detection assay for colorectal cancer micrometastases in lymph nodes (2004)

Inaccurate staging of colorectal cancer (CRC) has been attributed to the failure to detect lymph node metastases by conventional pathology. We have previously reported the use of lymphatic mapping to accurately identify those lymph nodes most likely to harbor micrometastatic disease and permit focused pathologic examination. Mutation of K-ras allele at codons 12 or 13 occurs frequently in early stages of CRC development. The purpose of our study was to assess sentinel lymph nodes (SLN) for occult CRC micrometastases using a unique peptide nucleic acid (PNA) clamp PCR assay specific for K-ras mutations. Seventy-two paraffin-embedded primary CRC and paired SLN were evaluated by PNA clamp PCR for K-ras mutations. Thirty primary tumors (42%) were positive for K-ras mutations, and in 5 of these cases the SLN were positive for metastases by Hematoxylin and Eosin staining. PNA clamp PCR identified occult metastases in an additional 6 patients, upstaging 24% of K-ras positive primary CRCs (p = 0.014). No K-ras mutations were detected among the 20 noncancer lymph nodes assessed. This study demonstrates the utility, specificity and sensitivity of PNA clamp PCR assay in identifying occult micrometastases in the SLN of CRC patients by single-base mutation analysis.
Intermediaries: New Places for Producing and Manipulating Web Content. (1998)

We propose a new approach to programming web applications that increases the web&apos;s computational power, the web&apos;s flexibility, and web programmer productivity. Whereas web servers have traditionally been responsible for producing all content, intermediaries now provide new places for producing and manipulating web data. We define intermediaries as computational elements that lie along the path of a web transaction. In this paper, we describe the fundamental ideas behind intermediaries and provide a collection of example applications. We also describe WBI, an implemented architecture for building intermediaries that we have used to construct many applications, including personal histories, password management, image distillation, collaborative filtering, targeted advertising, and web advising.
Virtual radiographs computed from TACT® volume data as a gold standard for image registration prior to subtraction (2002)

OBJECTIVE To develop a three-dimensional (3D) model for quantitative analysis of image subtraction methods simulating clinical conditions and relevant to dental radiology. METHOD A high-resolution volume representation of a formalin-preserved segment of a human maxilla was synthesized from a set of 51 digital radiographs equidistantly covering the entire sampling aperture by means of Tuned-Aperture Computed Tomography (TACT). Two-dimensional (2D) projection renderings of a 3D model were generated yielding arbitrary but well-known 2D projections with, and without, structured noise producing 'virtual radiographs'. RESULTS Virtual radiographs were found to be similar to actual clinical images with respect to appearance, structure, and texture. Because the TACT reconstruction process allows all possible positions and orientations of source, specimen, and image plane to be simulated with negligible under sampling over a reasonable range of solid angles (sampling aperture), the resulting 3D model provided a rigorous method for establishing a truly objective gold standard (ground truth) for testing different registration techniques. CONCLUSIONS TACT image registration can be assessed quantitatively by comparing actually observed vs theoretically professed parameters that presumably constrain the underlying projection geometries. Other attributes that vary from one method to the next, such as the use of nonlinear or region-specific techniques to facilitate registration, likewise, now can be rigorously measured by context-based methods such as quantitative determination of image similarity. Hence, a 3D model that renders idealized virtual radiographs from any desired projection geometry makes possible truly objective comparison of various digital subtraction techniques.
Aqueous Phase Behavior of a 1-<i>O</i>-Phytanyl-β-<scp>d</scp>-xyloside/Water System. Glycolipid-Based Bicontinuous Cubic Phases of Crystallographic Space Groups <i>Pn</i>3<i>m</i> and <i>Ia</i>3<i>d</i> (2004)

Temperature- and concentration-dependent aqueous phase diagram of a novel alkylglycoside, 1-O-phytanyl-beta-D-xyloside (beta-Xyl(Phyt)), was studied using small-angle X-ray scattering, polarizing optical microscopy, and differential scanning calorimetry. The phases found in this system include an Lc phase, an Lalpha phase, an HII phase, two inverted cubic phases of crystallographic space groups Pn3m and Ia3d, and a fluid isotropic phase, FI. The phase diagram of the beta-Xyl(Phyt)/water system is similar to that for the 1-monooleylglycerol (MO)/water system, suggesting that the phase behavior is largely determined by the overall molecular shape rather than the details of surfactant molecular structure. Moreover, the structural parameters of the beta-Xyl(Phyt) liquid crystals are also similar to those of the MO/water, due primarily to the similar molecular dimensions of two molecules. As compared to the MO/water system, however, the beta-Xyl(Phyt)/water system displays a lower value of TK ( approximately 8.(5) degrees C) and a wider temperature window for the mesophases (8.(5)-120 degrees C). Moreover, beta-Xyl(Phyt) is chemically more robust than MO, as the ether linkage is more stable against hydrolysis than the ester linkage and the phytanyl chain is fully saturated.
Effect of Photopolymerization on the Rate of Photocrosslink in Chalcone-based Oligomeric Compounds (2001)

Sang Joon Oh, Si Young Ban, and Kwang Yong OhCollege of Environment and Applied Chemistry, Materials Center for Information Technology, Kyung Hee University, Yongin, Kyungki 449-701, KoreaReceived August 13, 2001A new photochemically bifunctional epoxy and dimethacrylate compounds were synthesized for investigatingthe photosensitivity under UV irradiation. Photosensitivity of the synthesized chalcone-based compounds wasinvestigated by using UV-visible absorption and Fourier transformed infrared (FT-IR) spectroscopies. Theresult of spectroscopic analysis substantiated that the two chalcone-based compounds have functions of bothphotocrosslinking and photopolymerization by UV exposure in the presence of photoinitiator. Two kinds ofphotochemical reactions proceeded competitively during UV exposure. Particularly, we studied the effect ofphotopolymerization on the rate of photocrosslink due to cycloaddition in the two oligomeric compounds.Keywords : Photosensitivity, Chalcone-epoxy, Chalcone-dimethacrylate, Photocrosslink, Photopolymerization.IntroductionPhotoreactive materials have recently gained a remarkableinterest since the photochemical reactions in organic materialscan induce many changes in physicochemical properties suchas solubility, optical transparency, dielectric constant, andrefractive index.
Novel two-component regulatory systems play a role in biofilm formation of Lactobacillus reuteri rodent isolate 100-23 (2014)

This study characterized the two-component regulatory systems encoded by bfrKRT and cemAKR, and assessed their influence on biofilm formation by Lactobacillus reuteri 100-23. A method for deletion of multiple genes was employed to disrupt the genetic loci of two-component systems. The operons bfrKRT and cemAKR showed complementary organization. Genes bfrKRT encode a histidine kinase, a response regulator and an ATP-binding cassette-type transporter with a bacteriocin-processing peptidase domain, respectively. Genes cemAKR code for a signal peptide, a histidine kinase and a response regulator, respectively. Deletion of single or multiple genes in the operons bfrKRT and cemAKR did not affect cell morphology, growth or the sensitivity to various stressors. However, gene disruption affected biofilm formation; this effect was dependent on the carbon source. Deletion of bfrK or cemA increased sucrose-dependent biofilm formation in vitro. Glucose-dependent biofilm formation was particularly increased by deletion of cemK. The expression of cemK and cemR was altered by deletion of bfrK, indicating cross-talk between these two regulatory systems. These results may contribute to our understanding of the genetic factors related to the biofilm formation and competitiveness of L. reuteri in intestinal ecosystems.
Regulatory subunits of cAMP-dependent protein kinases are degraded after conjugation to ubiquitin: a molecular mechanism underlying long-term synaptic plasticity. (1993)

In Aplysia, behavioral sensitization of defensive reflexes and the underlying presynaptic facilitation of sensory-to-motor neuron synapses lasts for several minutes (short term) or days to weeks (long term). Short-term sensitization has been explained by modulation of ion-channel function through cAMP-dependent protein phosphorylation. Long-term facilitation requires additional molecular changes including protein synthesis. A key event is the persistent activation of the cAMP-dependent protein kinase at baseline concentrations of cAMP. This activation is due to selective loss of regulatory (R) subunits of PKA without any change in catalytic (C) subunits. To understand the molecular mechanisms that produce the loss of R subunits in long-term facilitation, we investigated how R subunits are degraded in extracts of Aplysia nervous tissue and in rabbit reticulocyte lysates. Degradation of Aplysia R subunits requires ATP, ubiquitin, and a particulate component that appears to be the proteasome complex. Degradation is blocked by hemin, which causes the accumulation of high molecular weight derivatives of R subunits that are likely to be ubiquitin conjugates of R subunits and intermediates in the degradative pathway. We also show that vertebrate RI and RII subunits can be degraded through the ubiquitin pathway. We suggest that degradation is initiated by cAMP, which causes the holoenzyme to dissociate and, further, that the altered R-to-C ratio in Aplysia sensory neurons is maintained in long-term facilitation by newly synthesized proteins that help target R subunits for accelerated degradation.
Commute UX: Voice Enabled In-car Infotainment System (2009)

Voice enabled dialog systems are well suited for in-car applications. Driving is an eyes-busy and hands-busy task and the only wideband communication channel left is speech. Such systems are in the midst of a transformation from a cool gadget to an integral part of the modern automobile. In this paper we highlight the major requirements for an in-car dialog system including usability during conditions of cognitive load, efficiency though multimodal user interface, dealing with locations, handling the noise in the car with better sound capture and robust speech recognition. We then present Commute UX, our prototype multi-modal dialog system for in-car infotainment system.
Spotting technique, new potassium-polymer blend give inexpensive shale inhibition (1986)

A new shale-control compound, combined with a novel method of application, can make cheap mud systems inhibitive. The compound is a nonionic polymer combined with a potassium phosphate complex. The preferred treatment is to spot small concentrations in open hole prior to trips. Formulating inhibitive water-based drilling fluids has been a goal of the mud industry since its inception. Early research focused on incorporating a single new additive into existing mud systems. Then, ''systems'' approaches evolved when the main additive created adverse side effects that necessitated a second additive, which in turn required a third additive.
Messenger RNA 3' End Formation in Plants (1994)

Crop loss due to soil salinization is an increasing threat to agriculture worldwide. This review provides an overview of cellular and physiological mechanisms in plant responses to salt. We place cellular responses in a time- and tissue-dependent context ...Read More