gebhart/patrepeval huggingface dataset:
A microfluorometric assay for the measurement of <i>de novo</i> DNA synthesis in individual cells. (1981)

A microfluorometric assay has been developed for the quantitation of de novo DNA synthesis. The assay reliably detects newly synthesized DNA in individual cells and provides a relative measure of the proportion of new DNA in each cell. When combined with microscopic techniques for cell identification, selected subpopulations can be examined in samples containing a complex mixture of interacting cell types. The assay employs the quenching of Hoechst-33258 fluorescence by bromodeoxyuridine incorporated into newly synthesized DNA. The ability of BUdR to quench H-33258 fluorescence is lost after a brief exposure of ultraviolet fluorescence excitation. Therefore, quenched and unquenched fluorescence intensity measurements from the same cell can be compared. Increases in fluorescence intensity occur only in cells that have synthesized DNA after the addition of BUdR. In addition the change in fluorescence intensity is proportional to the degree of BUdR substitution within the range of the assay and provides a relative measure of DNA synthesis.
Probing the Near-Field Inductive Coupling in Broadside Coupled Terahertz Metamaterials (2016)

We discuss near-field coupling between the pair of split ring resonators (SRRs) in broadside coupled terahertz (THz) metamaterials and examine resonance split and tuning of resonances in such metamaterials system. The metamolecule design is comprised of two orthogonally twisted broadside coupled SRRs separated by a thin microscale polyimide layer. We analyze the interaction between the metalayers numerically and analytically by displacing the top resonator w.r.t. the bottom resonator both in the horizontal and vertical directions. The THz transmission through proposed configuration results in the split of fundamental resonances due to the resonance mode hybridization effect. In the case of successive horizontal displacements between the resonators, we observe a complete shift from the coupled to uncoupled state, however, in vertical displacements, we notice the transition from coupled to uncoupled state at much larger displacement. We employed a semianalytical transmission line model to understand and describe the coupling aspect between the bilayer resonators and found that it corroborates the numerical findings. The ability to tune resonance behavior that we have examined through this work, could be significant in the development of frequency tunable THz devices and build other promising applications such as THz modulators and antennas in the near future.
Molecular Forms of Hypothalamic Ghrelin and Its Regulation by Fasting and 2-Deoxy-d-Glucose Administration (2005)

Ghrelin, an endogenous ligand for the GH secretagogue receptor, is a hormone expressed in stomach and other tissues, such as hypothalamus, testis, and placenta. This hormone acts at a central level to stimulate GH secretion and food intake. Little is known, however, about the molecular forms and physiological roles of ghrelin within the hypothalamus. In this report, we detail the molecular forms, mRNA expression patterns, and peptide contents of ghrelin within the rat hypothalamus. Using the combination of reverse-phase HPLC and ghrelin-specific RIA, we determined that the rat hypothalamus contains both n-octanoyl-modified and des-acyl ghrelins. Fasting for 24 and 48 h significantly decreased ghrelin mRNA expression in the hypothalamus to 24% and 28% of control values, respectively. Both n-octanoyl-modified and des-acyl ghrelin content in the hypothalamus decreased after 24 and 48 h of fasting. These results contrast the changes in gastric ghrelin after fasting, which decreased in content despite increased mRNA expression. Two hours after injection of 2-deoxy-d-glucose (2-DG), a selective blocker of carbohydrate metabolism, ghrelin peptide levels also decreased. Thus, induction of glucoprivic states, such as fasting and 2-DG treatment, decreased ghrelin gene expression and peptide content within the hypothalamus.
Investigations regarding the maskless pendeo-epitaxial growth of GaN films prior to coalescence (2002)

Pendeo-epitaxy employs lateral growth from etched seed forms to achieve a marked reduction in dislocation density in a material. In this research, high-resolution X-ray diffraction and atomic force microscopy of GaN stripes and the laterally grown wings confirmed transmission electron microscopy results regarding the reduction in dislocations in the latter regions. Micro-Raman and X-ray diffraction measurements showed the wings to be tilted /spl les/0.15/spl deg/ due to tensile stresses in the stripes induced primarily by the mismatch in the coefficients of thermal expansion between the GaN stripe and the SiC substrate. A strong, low-temperature D/spl deg/X peak at /spl ap/3.466 eV with a FWHM of /spl les/300 /spl mu/eV was measured in the wing material by micro-photoluminescence. Films grown at 1020/spl deg/C exhibited similar vertical [0001] and lateral [112~0] growth rates. Increasing the growth temperature increased the latter due to the higher thermal stability of the (112~0) GaN and initiated growth of spiral hillocks on the (0001) surface of the stripes. The latter were due to adatom diffusion to heterogeneous steps previously nucleated at the intersections of pure screw or mixed dislocations. The (112~0) surface was atomically smooth under all growth conditions with a root mean square roughness value of 0.17 nm.
Simulation and Implementation of High-Performance Collision Warning System for Motor Vehicle Safety Using Embedded ANFIS Prediction (2008)

A good driver-vehicle interface needs two functions: (a) how to effectively prevent traffic crash and (b) how to record event data precisely for facilitating the traffic crash investigation after accident. This study is to explore how to realize high-performance collision warning system (CWS), providing the precaution against traffic crash in transit. An embedded adaptive network-based fuzzy inference system (ANFIS) built in XScale-NAV270 platform was employed to realize collision warning system and we also installed motor vehicle event data recorder (MVEDR). Finally, simulation and verification of the proposed approach were successfully done to achieve better accuracy and more effectiveness on warning operation and event data record to motor vehicle.
Several Novel N-Donor Tridentate Ligands Formed in Chemical Studies of New<i>fac</i>-Re(CO)<sub>3</sub>Complexes Relevant to<i>fac</i>-<sup>99m</sup>Tc(CO)<sub>3</sub>Radiopharmaceuticals: Attack of a Terminal Amine on Coordinated Acetonitrile (2010)

To evaluate syntheses of fac-[Re(CO)(3)L](+) complexes in organic solvents, we treated fac-[Re(CO)(3)(CH(3)CN)(3)]PF(6)/BF(4) in acetonitrile with triamine ligands (L). When L had two primary or two tertiary terminal amine groups, the expected fac-[Re(CO)(3)L](+) complexes formed. In contrast, N,N-dimethyldiethylenetriamine (N,N-Me(2)dien) formed an unusual compound, fac-[Re(CO)(3)(DAE)]BF(4) {DAE = (Z)-N'-(2-(2-(dimethylamino)ethylamino)ethyl)acetimidamide = (Me(2)NCH(2)CH(2))NH(CH(2)CH(2)N=C(NH(2))Me)}. DAE is formed by addition of acetonitrile to the N,N-Me(2)dien terminal primary amine, converting this sp(3) nitrogen to an sp(2) nitrogen with a double bond to the original acetonitrile sp carbon. The three Ns bound to Re derive from N,N-Me(2)dien. The pathway to fac-[Re(CO)(3)(DAE)]BF(4) is suggested by a second unusual compound, fac-[Re(CO)(3)(MAE)]PF(6) {MAE = N-methyl-N-(2-(methyl-(2-(methylamino)ethyl)amino)ethyl)acetimidamide = MeN(H)-CH(2)CH(2)-N(Me)-CH(2)CH(2)-N(Me)-C(Me)=NH}, isolated after treating fac-[Re(CO)(3)(CH(3)CN)(3)]PF(6) with N,N',N''-trimethyldiethylenetriamine (N,N',N''-Me(3)dien). MAE chelates via a terminal and a central sp(3) N from N,N',N''-Me(3)dien and via one sp(2) NH in a C(Me)=NH group. This group is derived from acetonitrile by addition of the other N,N',N''-Me(3)dien terminal amine to the nitrile carbon. This addition creates an endocyclic NMe group within a seven-membered chelate ring. The structure and other properties of fac-[Re(CO)(3)(MAE)]PF(6) allow us to propose a reaction scheme for the formation of the unprecedented DAE ligand. The new compounds advance our understanding of the spectral and structural properties of Re analogues of (99m)Tc radiopharmaceuticals.
Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity (2014)

Nanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines. Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines. Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects.
A tensor-based algorithm for high-order graph matching (2009)

This paper addresses the problem of establishing correspondences between two sets of visual features using higher-order constraints instead of the unary or pairwise ones used in classical methods. Concretely, the corresponding hypergraph matching problem is formulated as the maximization of a multilinear objective function over all permutations of the features. This function is defined by a tensor representing the affinity between feature tuples. It is maximized using a generalization of spectral techniques where a relaxed problem is first solved by a multi-dimensional power method, and the solution is then projected onto the closest assignment matrix. The proposed approach has been implemented, and it is compared to state-of-the-art algorithms on both synthetic and real data.
Deficient DNA Damage Signaling Leads to Chemoresistance to Cisplatin in Oral Cancer (2012)

Abstract Activation of the cellular DNA damage response (DDR) is an important determinant of cell sensitivity to cisplatin and other chemotherapeutic drugs that eliminate tumor cells through induction of DNA damage. It is therefore important to investigate whether alterations of the DNA damage-signaling pathway confer chemoresistance in cancer cells and whether pharmacologic manipulation of the DDR pathway can resensitize these cells to cancer therapy. In a panel of oral/laryngeal squamous cell carcinoma (SCC) cell lines, we observed deficiencies in DNA damage signaling in correlation with cisplatin resistance, but not with DNA repair. These deficiencies are consistent with reduced expression of components of the ataxia telangiectasia mutated (ATM)-dependent signaling pathway and, in particular, strong upregulation of Wip1, a negative regulator of the ATM pathway. Wip1 knockdown or inhibition enhanced DNA damage signaling and resensitized oral SCC cells to cisplatin. In contrast to the previously reported involvement of Wip1 in cancer, Wip1 upregulation and function in these SCC cells is independent of p53. Finally, using xenograft tumor models, we showed that Wip1 upregulation promotes tumorigenesis and its inhibition improves the tumor response to cisplatin. Thus, this study reveals that chemoresistance in oral SCCs is partially attributed to deficiencies in DNA damage signaling, and Wip1 is an effective drug target for enhanced cancer therapy. Mol Cancer Ther; 11(11); 2401–9. ©2012 AACR.
The World Survey of Cardiac Pacing and Cardioverter Defibrillators: Calendar Year 1997 ‐ Asian Pacific, Middle East, South America, and Canada (2001)

A comprehensive cardiac pacing and ICD survey of the Asian Pacific region, Middle East, South America, and Canada was undertaken for calendar year 1997. Thirteen countries in the Asian Pacific region contributed to the survey. There were also two countries from the Middle East, three from South America, and Canada. Accurate numbers of new implants and replacements were obtained for cardiac pacemakers. In the Asian Pacific region, Japan with almost 20,000 implants, had the largest number of pacemaker implants, but Australia had the highest implant rate with 345 new implants per million population. However, Canada and Uruguay had higher new implant rates per million. The major indication for implantation of a cardiac pacemaker was high degree atrioventricular block. There was a high incidence of single chamber ventricular pacing in the developing countries of Asia. Only a few countries used substantial numbers of single lead VDD and AAIR pacing systems. Pacing leads were predominantly transvenous, bipolar, passive fixation, and steroid eluting. Active-fixation leads were also used in the atrium. With ICD use, Australia had the highest new implant rate per million population. The major indications were sudden death and ventricular arrhythmias. A group of enthusiastic survey coordinators has now been established. It is hoped to continue physician and country recruitment, particularly in the Middle East and South American regions to establish a fully global experience of cardiac pacing and ICD use.
Registries for Evaluating Patient Outcomes: A User's Guide [Internet] (2014)

This User's Guide is intended to support the design, implementation, analysis, interpretation, and quality evaluation of registries created to increase understanding of patient outcomes. For the purposes of this guide, a patient registry is an organized system that uses observational study methods to collect uniform data (clinical and other) to evaluate specified outcomes for a population defined by a particular disease, condition, or exposure, and that serves one or more predetermined scientific, clinical, or policy purposes. A registry database is a file (or files) derived from the registry. Although registries can serve many purposes, this guide focuses on registries created for one or more of the following purposes: to describe the natural history of disease, to determine clinical effectiveness or cost-effectiveness of health care products and services, to measure or monitor safety and harm, and/or to measure quality of care.Registries are classified according to how their populations are defined. For example, product registries include patients who have been exposed to biopharmaceutical products or medical devices. Health services registries consist of patients who have had a common procedure, clinical encounter, or hospitalization. Disease or condition registries are defined by patients having the same diagnosis, such as cystic fibrosis or heart failure.
Homogeneous Assays of Second Messenger Signaling and Hormone Secretion Using Thermofluorimetric Methods That Minimize Calibration Burden (2017)

Homogeneous assays are characterized by rapidity, low cost, and simple workflows. However, relatively few specialized homogeneous platforms have garnered significant use in biological studies. Inconsistencies in matrix interferences, limited multiplexability, and the requirement for specialized instrumentation are among the various reasons for delayed acceptance. Recently, we have shown that DNA-driven protein assays using thermofluorimetric analysis (TFA) can limit matrix interference and promote multiplexing, all while requiring only a standard qPCR instrument for readout. Here, we show that homogeneous, one step (mix-and-read) TFA methods can be extended to the analysis of both a small molecule second messenger, cyclic adenosine monophosphate (cAMP), and a downstream cell-secreted hormone, insulin. Differential thermal analysis of DNA melting in these assays allowed analytical discrimination of background and signal without physical separation. The direct-readout, differential nature of TFA also promoted assay consistency and minimized calibration burden; analyte response curves were shown to be highly repeatable for up to 7 months. TFA protocols were validated by homogeneous quantification of both cAMP and insulin from single pancreatic islets undergoing a variety of treatments (glucose, KCl, glucose-responsive insulinotropic peptide (GIP), forskolin) that act upon glucose transporters, potassium and calcium channels, and G-protein-coupled receptors to modulate exocytosis. The results of this study suggest that TFA should be applicable to homogeneous quantification of a variety of small molecule messengers and protein analytes with standard instrumentation, thereby simplifying workflows in studies of cell-signaling cascades.
Urethral Replacement with Tunica Vaginalis: A Pilot Study (1989)

A vascularized tube flap of tunica vaginalis was used to replace the penile urethra in 12 rabbits. Ten flaps were available for assessment, 2 flaps became necrotic as a result of compression of the vascular pedicle and 1 rabbit had a proximal anastomotic stricture. The healing process was satisfactory clinically and histologically in the remaining 7 flaps.
Main HCI issues for the design of interfaces for ubiquitous interactive multimedia broadcast (2004)

article Share on Main HCI issues for the design of interfaces for ubiquitous interactive multimedia broadcast Authors: Anxo Cereijo Roibás University of Brighton, Moulsecoomb, United Kingdom University of Brighton, Moulsecoomb, United KingdomView Profile , Riccardo Sala Politecnico di Milano University, Milan, Italy Politecnico di Milano University, Milan, ItalyView Profile Authors Info & Claims InteractionsVolume 11Issue 2March + April 2004 pp 51–53https://doi.org/10.1145/971258.971274Online:01 March 2004Publication History 9citation1,769DownloadsMetricsTotal Citations9Total Downloads1,769Last 12 Months219Last 6 weeks15 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Synthesis of Isotopically Labeled Arachidonic Acids To Probe the Reaction Mechanism of Prostaglandin H Synthase (2002)

Prostaglandin H synthase (PGHS) catalyzes the conversion of arachidonic acid to prostaglandin G(2) in the cyclooxygenase reaction. The first step of the mechanism has been proposed to involve abstraction of the pro-S hydrogen atom from C13 to generate a pentadienyl radical spanning C11-C15. We report here the synthesis of six site-specifically deuterated arachidonic acids to investigate the structure of the radical intermediate. The preparation of these compounds was achieved using a divergent scheme that involved one advanced intermediate for all targets. The synthetic design introduced the label late in the routes and allowed the utilization of common synthetic intermediates in the preparation of various targets. Both 13(R)- and 13(S)-deuterium-labeled arachidonic acids were synthesized in high enantiomeric purity as deduced from soybean lipoxygenase assays and mass spectrometric analysis of the resulting enzymatic products. Each synthetic compound was reacted under anaerobic conditions with the wide singlet tyrosyl radical of PGHS-2 to generate a radical intermediate that was analyzed by EPR. Deuterium substitution at positions 11, 13(S), and 15 resulted in the loss of one hyperfine interaction, indicating that the protons at these positions interact with the unpaired electron. Simulation of the spectra was achieved with one set of parameters that are consistent with the assignment of a pentadienyl radical. Use of 16-[(2)H(2)]-arachidonic acid indicated that only one of the protons at C16 gives rise to a strong hyperfine interaction. The findings are discussed in the context of two proposed mechanisms for the cyclooxygenase reaction.
SYNTHESIS AND STUDY OF CORROSION PERFORMANCE OF EPOXY COATING CONTAINING MULTI-WALLED CARBON NANOTUBE/ POLY ORTHO AMINOPHENOL NANOCOMPOSITE (2016)

The epoxy coatings containing multi-walled carbon nanotube/ poly ortho aminophenol nanocomposite were prepared and used as anticorrosive coatings. The nanocomposites with different contents of carbon nanotube were synthesized in a solution of sodium dodecyl sulfate and ammonium peroxy disulfate as a surfactant and an oxidant, respectively. The morphology and structural properties were confirmed by Fourier transform infrared spectroscopy and scanning electron microscopy methods. The mean size of nanocomposite particles was 20-35 nm determined by scanning electron microscopy. The epoxy coatings containing the nanocomposites were applied over mild steel panels and their corrosion performance was investigated using electrochemical impedance spectroscopy and potentiodynamic polarization measurements in a 3.5 % sodium chloride solution. The results showed that epoxy coatings consisting of nanocomposite with 1 wt.% multi-walled carbon nanotube exhibited higher anticorrosive properties than other prepared coatings of different carbon nanotube contents, which could be due to the strong interaction between the mild steel surface and the conjugated nanocomposite.
Noonan Syndrome: Clinical Features, Diagnosis, and Management Guidelines (2010)

Noonan syndrome (NS) is a common, clinically and genetically heterogeneous condition characterized by distinctive facial features, short stature, chest deformity, congenital heart disease, and other comorbidities. Gene mutations identified in individuals with the NS phenotype are involved in the Ras/MAPK (mitogen-activated protein kinase) signal transduction pathway and currently explain ∼61% of NS cases. Thus, NS frequently remains a clinical diagnosis. Because of the variability in presentation and the need for multidisciplinary care, it is essential that the condition be identified and managed comprehensively. The Noonan Syndrome Support Group (NSSG) is a nonprofit organization committed to providing support, current information, and understanding to those affected by NS. The NSSG convened a conference of health care providers, all involved in various aspects of NS, to develop these guidelines for use by pediatricians in the diagnosis and management of individuals with NS and to provide updated genetic findings.
The formation of GaN dots on AlxGa1−xN surfaces using Si in gas-source molecular beam epitaxy (1998)

Nanoscale GaN dots were successfully formed on AlxGa1−xN/6H-SiC(0001) surfaces by gas-source molecular beam epitaxy. It was found that the growth mode can be changed by introducing Si before GaN growth, where the Si is believed to play an important role in the change of the AlxGa1−xN surface free energy. Without introducing Si, the GaN growth mode was two dimensional and (1×3) reconstruction was observed. The growth mode of GaN was changed from two-dimensional to three-dimensional by introducing Si on the AlxGa1−xN surface. In situ reflection high-energy electron diffraction and atomic force microscopy observations were used to monitor and characterize the growth processes and surface morphology.