gebhart/patrepeval huggingface dataset:
Clinical, genomic, and imaging predictors of malignancy: Analysis of the first U.S. cooperative group prospective clinical trial in asymptomatic monoclonal gammopathies (SWOG S0120). (2013)

8515 Background: Asymptomatic monoclonal gammopathies (AMG) are the most common plasma cell dyscrasia classified as either monoclonal gammopathy of undetermined significance (MGUS) or asymptomatic multiple myeloma (AMM). Clinical outcome in AMGs can be highly variable and there is an unmet need to identify newer clinical, genomic and imaging parameters from prospective studies to guide patient management. Methods: We analyzed clinical, genomic and imaging data from AMG patients (n=334) enrolled in a prospective observational clinical trial (S0120) conducted under the auspices of SWOG. Baseline data from clinical variables, as well as gene expression profiles of purified tumor cells and the findings on magnetic resonance imaging (MRI) were correlated with the risk of progression to symptomatic myeloma (MM) requiring therapy. Results: In addition to serum M spike, percent bone marrow plasma cells and the ratio of involved/uninvolved serum free light chains, the level of serum beta-2-microglobulin was associated with an increased risk of progression to clinical myeloma requiring therapy. Gene expression profiles (GEP) of purified tumor cells revealed that all of the known molecular GEP subtypes of human MM are also represented in the precursor phase. An increased risk score (> - 0.26) based on a 70-gene signature (GEP70) was an independent predictor of the risk of progression to clinical MM requiring therapy. The presence of focal lesions on MRI also conferred an increased risk of disease progression but was not independent of other clinical and genomic features. Conclusions: These data represent the first comprehensive evaluation of clinical, genomic and imaging features of AMGs in the context of a prospective US-cooperative group trial, and demonstrate that while all genetic subtypes of MM have a precursor phase, genetic signatures previously associated with high risk myeloma also predict the risk of progression to clinical malignancy requiring therapy. These findings suggest the need to integrate both clinical assessment of tumor bulk and genomic properties of tumor cells in the clinical management of these patients. Clinical trial information: NCT00900263.
Fatigue compensation of the electromyographic signal for prosthetic control and force estimation (1993)

During a sustained muscle contraction, the amplitude of electromyographic (EMG) signals increases and the spectrum of the EMG signal shifts toward lower frequencies. These effects are due to muscular fatigue and can cause problems in the control of myoelectric prostheses and in the estimation of contraction level from the EMG signal. It has been well known that the fatigue effects can be explained by the conduction velocity changes during the fatigue process and by the idea that the conduction velocity is linearly proportional to the median frequency of EMG signals. Hence the fatigue process can be monitored by measuring the median frequency. A fatigue compensation preprocessor has been developed. It uses the widely accepted power spectrum density model of EMG signals that contains the conduction velocity as a measure of fatigue. It was verified that the preprocessor scales down the amplitude of the fatigued EMG signal and decompresses the spectrum. Hence, the preprocessor eliminates the increase in amplitude and the shift in frequency and enables consistent EMG signals to be used to control prostheses.
Fungal β-glucosidase expression in <i>Saccharomyces cerevisiae</i> (2012)

Recombinant Saccharomyces cerevisiae strains expressing β-glucosidases from Thermoascus aurantiacus (Tabgl1) and Phanerochaete chrysosporium (PcbglB and Pccbgl1) were constructed and compared to S. cerevisiae Y294[SFI], previously identified as the best β-glucosidase-producing strain. The PcbglB was also intracellularly expressed in combination with the lac12 lactose permease of Kluyveromyces lactis in S. cerevisiae Y294[PcbglB + Lac12]. The recombinant extracellular β-glucosidases indicated maximum activity in the pH range 4-5 and temperature optima varying from 50 to 75 °C. The S. cerevisiae Y294[Pccbgl1] strain performed best under aerobic and anaerobic conditions, producing 2.6 times more β-glucosidase activity than S. cerevisiae Y294[SFI] and an ethanol concentration of 4.8 g l(-1) after 24 h of cultivation on cellobiose as sole carbohydrate source. S. cerevisiae Y294[Tabgl1] was unable to grow on cellobiose (liquid medium), whereas S. cerevisiae Y294[PcbglB + Lac12] exhibited limited growth.
Amino- and Nitrofluorescein Derivatives (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAmino- and Nitrofluorescein DerivativesRoger M. McKinney, Janet T. Spillane, and George W. PearceCite this: J. Org. Chem. 1962, 27, 11, 3986–3988Publication Date (Print):November 1, 1962Publication History Published online1 May 2002Published inissue 1 November 1962https://pubs.acs.org/doi/10.1021/jo01058a055https://doi.org/10.1021/jo01058a055research-articleACS PublicationsRequest reuse permissionsArticle Views567Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting (2016)

The functional maturation and preservation of hepatic cells derived from human induced pluripotent stem cells (hiPSCs) are essential to personalized in vitro drug screening and disease study. Major liver functions are tightly linked to the 3D assembly of hepatocytes, with the supporting cell types from both endodermal and mesodermal origins in a hexagonal lobule unit. Although there are many reports on functional 2D cell differentiation, few studies have demonstrated the in vitro maturation of hiPSC-derived hepatic progenitor cells (hiPSC-HPCs) in a 3D environment that depicts the physiologically relevant cell combination and microarchitecture. The application of rapid, digital 3D bioprinting to tissue engineering has allowed 3D patterning of multiple cell types in a predefined biomimetic manner. Here we present a 3D hydrogel-based triculture model that embeds hiPSC-HPCs with human umbilical vein endothelial cells and adipose-derived stem cells in a microscale hexagonal architecture. In comparison with 2D monolayer culture and a 3D HPC-only model, our 3D triculture model shows both phenotypic and functional enhancements in the hiPSC-HPCs over weeks of in vitro culture. Specifically, we find improved morphological organization, higher liver-specific gene expression levels, increased metabolic product secretion, and enhanced cytochrome P450 induction. The application of bioprinting technology in tissue engineering enables the development of a 3D biomimetic liver model that recapitulates the native liver module architecture and could be used for various applications such as early drug screening and disease modeling.
Human Placentae: View From an Immunological Bias (1989)

Human placentae form a major part of the interface between maternal and embryonic tissues. Placentae are morphologically complex organs that are lined with a trophoblastic tissue that provides anatomical constraints over which flows maternal blood. Maternal antibodies and immune cells thus contact the lining syncytiotrophoblast, and this paper considers some of the antigens that maternal immune components might encounter. The antigens have been grouped into those of unknown function, major histocompatibility antigens, and antigens of suspected function. The paper also details certain antigens of cytotrophoblasts. We discuss consequences of maternal immune responses to extraembryonic antigens and present evidence for amplification of these responses through the activation of hemostasis and fibrinolysis within the placenta and placental bed.
Requirements for Management of Overload in the Session Initiation Protocol (2008)

Overload occurs in Session Initiation Protocol (SIP) networks when proxies and user agents have insufficient resources to complete the processing of a request. SIP provides limited support for overload handling through its 503 response code, which tells an upstream element that it is overloaded. However, numerous problems have been identified with this mechanism. This document summarizes the problems with the existing 503 mechanism, and provides some requirements for a solution.
Optimization of urinary FDG excretion during PET imaging. (1999)

Hydrochlorothiazide increases urine volume without enhancing FDG excretion. The hydration of patients before PET scanning may lead to more FDG reaching the bladder. Reduction of bladder activity by more frequent voiding facilitated by increased urine volume in hydrated patients may be offset by increased delivery of FDG to the bladder. A preferable means of increasing urinary volume without increasing delivery of FDG to the bladder may be the use of a diuretic.
Purification of a Plasminogen Activator Inhibitor Indistinguishable from α<sub>1</sub>-Antitrypsin and an Urokinase Inhibitor in Pregnancy Plasma (1976)

An inhibitor of urokinase present in increased concentrations in plasma during pregnancy was purified. The inhibitor was identified by its pronounced effect on urokinase-induced fibrinolysis as opposed to the absence of effect on fibrinolysis induced by a tissue plasminogen activator. The inhibitor progressively inactivated urokinase upon incubation and was found to be indistinguishable from alpha1-antitrypsin.
Neural cell integration into 3D bioprinted skeletal muscle constructs accelerates restoration of muscle function (2020)

A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration.
Human plasma R-type vitamin B12-binding proteins. I. Isolation and characterization of transcobalamin I. TRANSCOBALAMIN III. and the normal granulocyte vitamin B12-binding protein. (1975)

Transcobalamin I and transcobalamin III have been purified approximately 6,000,000- and 3,000,000-fold, respectively, from normal human plasma using a purification scheme consisting of immunoadsorption, dialysis against 7.5 M guanidine HCl to remove endogenous vitamin B12, and affinity chromatography on vitamin B12-Sepharose. The two proteins were separated from each other subsequently by chromatography on DEAE-cellulose. The vitamin B12-binding protein present in granulocytes obtained from normal subjects has been purified approximately 5000-fold using affinity chromatography on vitamin B12-Sepharose as the sole purification technique. The final preparations of all three proteins were homogeneous based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Transcobalamin I and transcobalamin III belong to the R-typed class of vitamin B12-binding proteins and are indistinguishable from each other, and from the human granulocyte, milk, and saliva R-type vitamin B12-binding proteins, when studied by immunodiffusion with rabbit anti-human milk vitamin B12-binding protein sera. The carbohydrate compositions, expressed as moles of carbohydrate per mole of vitamin B12, of transcobalamin I, transcobalamin III, and the normal granulocyte vitamin B12-binding protein, respectively, are: sialic acid, 18, 11, 11; fucose, 9, 20, 24; galactose, 41, 51, 46; mannose, 24, 22, 20; galactosamine, 2, 2, 2; and glucosamine, 46, 54, 46. The high sialic acid content of transcobalamin I appears to account for the fact that this protein elutes after transcobalamin III and the normal granulocyte vitamin B12-binding protein during chromatography on DEAE-cellulose. This observation provides support for the hypothesis that differences among the R-type vitamin B12-binding proteins are due to differences in carbohydrate content. The similarities in carbohydrate composition and other properties of transcobalamin III and the granulocyte vitamin B12-binding protein provide support for the hypothesis that human plasma transcobalamin III is derived from granulocytes. The differences observed between transcobalamin I and the normal granulocyte vitamin B12-binding protein suggest that transcobalamin I may not be derived from granulocytes.
Timing-Failure Risk Assessment of UML Design Using Time Petri Net Bound Techniques (2010)

Software systems that do not meet their timing constraints can cause risks. In this work, we propose a comprehensive method for assessing the risk of timing failure by evaluating the software design. We show how to apply best practises in software engineering and well-known Time Petri Net (TPN) modeling and analysis techniques, and we demonstrate the effectiveness of the method with reference to a case study in the domain of real-time embedded systems. The method customizes the Australian standard risk management process, where the system context is the UML-based software specification, enriched with standard MARTE profile annotations to capture nonfunctional system properties. During the risk analysis, a TPN is derived, via model transformation, from the software design specification and TPN bound techniques are applied to estimate the probability of timing failure. TPN bound techniques are also exploited, within the risk evaluation and treatment steps, to identify the risk causes in the software design.
Optimizing beta-blocker use after myocardial infarction. (2000)

Although beta-adrenergic blockers can significantly reduce mortality after a myocardial infarction, these agents are prescribed to only a minority of patients. Underutilization of beta blockers may be attributed, in part, to fear of adverse effects, especially in the elderly and in patients with concomitant disorders such as diabetes or heart failure. However, studies have shown that such patients are precisely the ones who derive the greatest benefit from beta blockade. Advancing age or the presence of potentially complicating disease states is usually not a justification for withholding beta-blocker therapy. With use of cardioselective agents and through careful dosing and monitoring, the benefits of beta blockers after myocardial infarction far outweigh the potential risks in most patients.
The role of desensitization to capsaicin in chili pepper ingestion and preference (1981)

It has been suggested that part of the widespread preference for chih pepper can be explained as a desensitization of the chemical irritant receptors in the mouth, produced by continued exposure to chili pepper. To evaluate this possibility, we measure detection thresholds and salivary response of subjects who vary in liking for chili pepper and amount of exposure to it. The stimuli used are aqueous solutions of capsaicin, the purified piquant agent in chili peppers. We find very small but reliable and significant decreases in sensitivity to capsaicin for both measures by chili likers or users. We conclude that there is a slight desensitization effect, but also that it cannot be a major factor in explaining the acquisition of chili preference. There is too much overlap between groups, and chili likers claim to like chili because of its chemical irritant (“mouth burn”) effect, not in spite of it.
<title>Diagnosing the predisposition for diabetes mellitus by means of mid-IR spectroscopy</title> (2002)

The vicious circle of insulin resistance and hyperinsulinemia is considered to precede the manifestation of diabetes type-2 by decades and the corresponding cluster of risk factors is described as the 'insulin resistance syndrome' or 'metabolic syndrome'. Since the present diagnosis of insulin resistance is expensive, time consuming and cumbersome, there is a need for diagnostic alternatives. We conducted a clinical study on 129 healthy volunteers and 99 patients suffering from the metabolic syndrome. We applied mid-infrared spectroscopy to dried serum samples from these donors and evaluated the spectra by means of disease pattern recognition (DPR). Substantial differences were found between the spectra originating from healthy volunteers and those spectra originating from patients with the metabolic syndrome. A linear discriminant analysis was performed using approximately one half of the sample set for teaching the classification algorithm. Within this teaching set, a classification sensitivity and specificity of 84 percent and 81 percent respectively can be derived. Furthermore, the resulting discriminant function was applied to an independent validation of the remaining half of the samples. For the discrimination between 'healthy' and 'metabolic syndrome' a sensitivity and a specificity of 80 percent and 82 percent respectively is obtained upon validating the algorithm with the independent validation set.
Studying the Effects of Interference on GNSS Signals (2013)

Global Navigation Satellite System (GNSS) signals, such as those from United States Global Positioning System (GPS), Europe’s Galileo, and Russia’s Global Navigation Satellite System (GLONASS), typically have extremely low received signal strengths at the Earth’s surface and are therefore susceptible to a range of interference signals. Such interference examples include, but not limited to, intentional sources such as Personal Privacy Devices (PPD) and unintentional sources such as Digital Enhanced Cordless Telecommunications (DECT) and Long Term Evolution (LTE) signal. Recent studies into such interference sources have identified GNSS jamming as having a major impact on geo-location positioning and communications technologies. One such study is the U.K. government funded Sentinel trial which investigated the density of jamming technologies at various road-side locations across the U.K. Another is the THV Galatea trial conducted by the Ministry of Defence in 2009 which documented the impact of interference signals on maritime navigation and communication systems [6]. Assessing the vulnerability of GNSS signals to such interference sources in real world scenarios is a challenging and time-consuming task which is subject to numerous environmental uncertainties. This paper describes a synthetic test environment which can model the effects of interference sources on GNSS signals and thus provide accurate, repeatable control of the signal characteristics in the laboratory. The system provides real-time control of both the GNSS and interference signal characteristics including definition of the receiving vehicle dynamics, signal modulation type, on/off periods, power level and center frequency. Of particular interest is the ability to introduce custom or user-defined modulation types which enables easy utilization of application critical waveforms during testing. This paper demonstrates the effect of several common broadband noise signals, as might be seen with a PPD, on a GNSS receiver. The results and conclusions of the investigation are supported by examining the receiver’s carrier-to-noise density (C/N0) and performance impact as a result of the interference sources throughout the test cases.
Hot Stamping of a B-Pillar Outer from High Strength Aluminum Sheet AA7075 (2014)

<div class="section abstract"><div class="htmlview paragraph">This work demonstrates the feasibility of hot stamping a B-pillar outer panel from aluminum alloy 7075. AA7075 is characterized by a high strength to weight ratio with yield strengths comparable to those of DP and TRIP advanced high strength steels. Applications using AA7075 have typically been limited to the aerospace industry due to the high variable cost associated with forming and joining of these materials. A primary key to implementation in the automotive industry is the development of metal forming methods that produce non-compromised stamped parts at automotive manufacturing volumes and costs. This work explores the feasibility of die quenching a hot blank within a cold die as a means of delivering high strength aluminum sheet parts. A die made from kirksite was used to evaluate the hot stamping process for a B-pillar outer. After the forming/quenching operation, the parts were subjected to an artificial aging process to regain the properties of the T6-temper. The results show that hot stamping, followed by an adequate artificial aging treatment, produces a high strength component with material properties equivalent to the as-received T6 properties delivered by the material sheet supplier. In addition, the hot stamped B-pillar component demonstrated limited springback with a high level of dimensional accuracy. </div></div>
Preparation of monolithium acetylide in tetrahydrofuran. Reaction with aldehydes and ketones (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of monolithium acetylide in tetrahydrofuran. Reaction with aldehydes and ketonesM. Mark MidlandCite this: J. Org. Chem. 1975, 40, 15, 2250–2252Publication Date (Print):July 1, 1975Publication History Published online1 May 2002Published inissue 1 July 1975https://doi.org/10.1021/jo00903a029RIGHTS & PERMISSIONSArticle Views1106Altmetric-Citations136LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (452 KB) Get e-Alertsclose Get e-Alerts