gebhart/patrepeval huggingface dataset:
Trafficking-Mediated STING Degradation Requires Sorting to Acidified Endolysosomes and Can Be Targeted to Enhance Anti-tumor Response (2017)

STING is an endoplasmic reticulum (ER)-associated transmembrane protein that turns on and quickly turns off downstream signaling as it translocates from the ER to vesicles. How STING signaling is attenuated during trafficking remains poorly understood. Here, we show that trafficking-mediated STING degradation requires ER exit and function of vacuolar ATPase complex. Late-stage STING vesicles are sorted to Rab7-positive endolysosomes for degradation. Based on analysis of existing structures, we also identified the helix amino acid 281 (aa281)-297 as a motif required for trafficking-mediated STING degradation. Immuno-electron microscopy (EM) reveals the size and clustering of STING vesicles and topology of STING on the vesicle. Importantly, blockade of trafficking-mediated STING degradation using bafilomycin A1 specifically enhanced cyclic guanosine monophosphate (GMP)-AMP (cGAMP)-mediated immune response and anti-tumor effect in mice. Together, our findings provide biochemical and imaging evidence for STING degradation by the lysosome and pinpoint trafficking-mediated STING degradation as a previously unanticipated therapeutic target for enhancing STING signaling in cancer therapy.
Thermostable α-Diimine Nickel(II) Catalyst for Ethylene Polymerization: Effects of the Substituted Backbone Structure on Catalytic Properties and Branching Structure of Polyethylene (2009)

On the basis of the strategy of promoting thermostability of α-diimine nickel catalyst by ligand backbone framework, a series of α-diimine nickel(II) complexes with bulky camphyl or diaryl-substituted backbones, [2,6-(R2)2C6H3−N═C(R1)−C(R1)═N−2,6-(R2)2C6H3]NiBr2 (1a, R1 = Ph, R2 = CH3; 2a, R1 = 4-methylphenyl, R2 = CH3; 3a, R1 = 4-fluorophenyl, R2 = CH3; 4a, R1 = camphyl, R2 = CH3; 4b, R1 = camphyl, R2 = i-Pr), were synthesized and used as catalyst precursors for ethylene polymerization. Crystallographic analysis revealed that the bulky camphyl backbone has a valid steric-effect on the nickel center by blocking the axial site for the metal center and suppressing the potential rotation of the CAr−N bond. Ethylene polymerizations catalyzed by these nickel α-diimine complexes activated by MAO were systematically investigated and the influences of the substituted backbones as well as reaction temperature on the catalytic activity, molecular weight and branching structure of the polymers were evaluated. It was found that the catalysts containing a camphyl backbone have excellent thermal stability and polymer structure control for ethylene polymerizations. Even at 80 °C, the 4b/MAO system still kept high activity and relatively stable kinetics and produced high molecular weight polyethylene. Moreover, the branching degrees and branched chain distribution of the polyethylenes obtained by the complex could also be controlled by tuning the reaction temperature.
Lactoferrin: affinity purification from human milk and polymorphonuclear neutrophils using monoclonal antibody (II 2C) to human lactoferrin, development of an immunoradiometric assay using II 2C, and myelopoietic regulation and receptor-binding characteristics. (1986)

Several investigators have now confirmed our original report demonstrating the myelopoietic suppressive activity of lactoferrin (LF) in vitro. In order to further clarify this activity, we used the recently produced and purified neutralizing antibody (II 2C) to LF to set up an immunoradiometric assay specific for LF and to affinity purify LF from lysates of peripheral blood polymorphonuclear neutrophils (PMN) obtained from healthy donors. Iron-saturated purified PMN LF was as active as iron-saturated affinity purified milk LF as a suppressor of the release of granulocyte-macrophage colony stimulating factors (GM-CSF) from mononuclear human peripheral blood leukocytes. The activities of both the PMN LF and milk LF were inactivated by preincubation with monoclonal anti-LF antibody (II 2C). In order to evaluate the methods of iron saturation of LF in vitro as measures of their functional activities, milk LF was iron saturated by four different methods, including ferric citrate, ferric ammonium sulphate, ferric chloride with nitriloacetate, and ferric chloride alone. The functional characteristics of all four preparations of LF saturated with iron in vitro were relatively equal and were more active than native LF. Resident mouse peritoneal macrophages separated into subpopulations of GM-CSF-producing cells by velocity sedimentation were evaluated for their LF-receptor binding capacity and for sensitivity to the suppression of GM-CSF release by LF. Iron saturated LF suppressed release of GM-CSF from only those fractions containing LF-receptor bearing cells, although not all fractions containing cells bearing receptors for LF responded to the suppressive activity of LF. These studies provide further evidence for the myelopoietic regulatory activity in vitro of PMN-derived LF, which is mediated through populations of mononuclear phagocytes having receptors for LF.
On the application of multiple hypothesis tracking to the cyber domain (2011)

In cyberspace, attackers are becoming increasingly agile, coordinated, and advanced. In response, defenders have deployed cyber sensors in order to gain visibility into both network and host activities. However, the data generated by these sensors must be correlated to identify individual attacks. This is currently a highly manual process. Unfortunately, this approach does not scale as the number of endpoints increases, the number of networks grow, and limited skilled human analysts. Current state of the art analytic techniques have been developed to aid the human analyst in the association of sensor alerts to cyber attacks. However, these analytic systems require association decisions to be irrevocably made at the time of association. Our approach is to extend the multiple hypothesis tracking framework, from the standard kinematic domain, to the cyber domain. Combining the multiple hypothesis tracking framework with cyber association analytics will allow the formation of more accurate cyber attack tracks, reducing the computational burden currently residing on the human.
Laser Scattering Tomography on Magnetic CZ-Si for Semiconductor Process Optimization (2005)

Laser scattering tomography (LST) and band-to-band photoluminescence (PL) are applied for supporting a MEMS process optimization. Process wafers are based on magnetic CZ grown silicon material. LST allows the characterization of number-size distributions of oxygen precipitates in various stages of the process flow. Precipitation is shown to be affected by the design of high-temperature anneal post initial oxidation. PL gives useful information on relative concentration level and radial distribution of recombination centers within process wafers. The initial oxidation leads to significant reduction of recombination centers. The combined LST/PL information enables valuable conclusions towards process optimization.
Synthesis of 5-carboxymethyluridine. Nucleoside from transfer ribonucleic acid (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 5-carboxymethyluridine. Nucleoside from transfer ribonucleic acidJohn D. Fissekis and Frederick SweetCite this: Biochemistry 1970, 9, 16, 3136–3142Publication Date (Print):August 4, 1970Publication History Published online1 May 2002Published inissue 4 August 1970https://pubs.acs.org/doi/10.1021/bi00818a004https://doi.org/10.1021/bi00818a004research-articleACS PublicationsRequest reuse permissionsArticle Views309Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Equilibria between pyranoses and furanoses. II. Aldoses (1972)

The proton magnetic resonance spectra of the aldopentoses and aldohexoses were determined in deuterium oxide solution. The signals of the anomerio protons of the two furanose and the two pyranose forms were located; the identification of these signals is discussed. The proportions of the four forms in equilibrium solution were determined.
Bacteriocin of a Group A Streptococcus: Partial Purification and Properties (1973)

The production of bacteriocin-like inhibition by certain strains of group A streptococci was demonstrated during the growth of these organisms on solid nutrient media. Active bacteriocin could not be recovered from broth cultures, possibly because of its inactivation by streptococcal proteinase. The bacteriocin, streptocin A, produced by Streptococcus pyogenes strain FF-22 was partially purified by chemical precipitation and chromatography on carboxymethyl-cellulose. Streptocin A inhibited the growth of various gram-positive organisms but none of a wide range of different gram-negative strains. The bacteriocin was inactivated by proteolytic enzymes and, although labile in alkali, it was extremely stable to heating in mild acids.
SiGe HBTs Optimization for Wireless Power Amplifier Applications (2010)

This paper deals with SiGe HBTs optimization for power amplifier applications dedicated to wireless communications. In this work, we investigate the f T -BV CEO tradeoff by various collector optimization schemes such as epilayer thickness and dopant concentration, and SIC and CAP characteristics. Furthermore, a new trapezoidal base Germanium (Ge) profile is proposed. Thanks to this profile, precise control of Ge content at the metallurgical emitter-base junction is obtained. Gain stability is obtained for a wide range of temperatures through tuning the emitter-base junction Ge percent. Finally, a comprehensive investigation of Ge introduction into the collector (backside Ge profile) is conducted in order to improve the f T values at high injection levels.
Detecting Movement Type by Route Segmentation and Classification (2012)

Data about people movement is nowadays easy to collect by the GPS technology embedded in smartphones. GPS routes provide information about position, time and speed, but further conclusion requires either prior information or data analysis. We propose a method to detect the movement type by segmentat
Anisotropic interactions of a single spin and dark-spin spectroscopy in diamond (2005)

The nitrogen-vacancy (N-V) center in diamond is a promising atomic-scale system for solid-state quantum information processing. Its spin-dependent photoluminescence has enabled sensitive measurements on single N-V centers, such as: electron spin resonance, Rabi oscillations, single-shot spin readout and two-qubit operations with a nearby 13C nuclear spin. Furthermore, room temperature spin coherence times as long as 58 microseconds have been reported for N-V center ensembles. Here, we have developed an angle-resolved magneto-photoluminescence microscopy apparatus to investigate the anisotropic electron spin interactions of single N-V centers at room temperature. We observe negative peaks in the photoluminescence as a function of both magnetic field magnitude and angle that are explained by coherent spin precession and anisotropic relaxation at spin level anti-crossings. In addition, precise field alignment unmasks the resonant coupling to neighboring dark nitrogen spins that are not otherwise detected by photoluminescence. The latter results demonstrate a means of investigating small numbers of dark spins via a single bright spin under ambient conditions.
Process monitoring of fibre reinforced composites using a multi-measurand fibre-optic sensor (2015)

This paper reports on the design, fabrication, characterisation and deployment of a multi-measurand optical fibre sensor (MMS) that is capable of simultaneously monitoring strain, temperature, refractive index and cross-linking chemistry. The sensor design is based on the extrinsic fibre Fabry–Perot interferometer. A feature of this sensor system is that a conventional multi-channel fibre-coupled near-infrared spectrometer is used to monitor the four independent parameters. The issues relating to the measurement resolution of the individual sensors and the associated interrogation equipment are discussed. The MMS was embedded in between the fourth and fifth plies of an eight-ply E-glass plain-weave fabric. A commercially available thermosetting epoxy/amine resin system was used to impregnate the fabric layers manually. The laminated preform was vacuum-bagged and cured in an autoclave. The following parameters were monitored: the depletion rates of the epoxy and amine functional groups in the resin system; the temperature in close proximity to the “chemical sensor”; the evolution of strain; and the refractive index of the resin system. The effect of post-processing on the output from the embedded optical fibre sensors is also considered.
Industrialization of AMB systems with standard drive technology (2010)

Around two decades ago, active magnetic bearings (AMB) were introduced into O&G-applications, but never gained any relevant market share in large turbo machinery. This paper discusses how standard drive technology from machine tool applications can be used to control AMB systems. As a proof of the concept, results of prototype testing with a 5 MW, induction motor are presented.
Solubility of carbon dioxide and hydrogen sulfide in aqueous alkanolamines (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolubility of carbon dioxide and hydrogen sulfide in aqueous alkanolaminesRalph H. Weiland, Tanmoy Chakravarty, and Alan E. MatherCite this: Ind. Eng. Chem. Res. 1993, 32, 7, 1419–1430Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/ie00019a016https://doi.org/10.1021/ie00019a016research-articleACS PublicationsRequest reuse permissionsArticle Views1540Altmetric-Citations143LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Aircraft failure detection and identification using neural networks (1993)

In this paper, a neural network is proposed as an approach to the task of failure detection following damage to an aerodynamic surface of an aircraft flight control system. Several drawbacks of other failure detection techniques can be avoided by taking advantage of the flexible learning and generalization capabilities of a neural network. This structure, used for state estimation purposes, can be designed and trained on line in flight and generates a residual signal indicating the damage as soon as it occurs. From an analysis of the cross-correlation functions between some key state variables, the identification of the damage type can also be achieved. The results of a nonlinear numerical simulation for a damaged control surface are reported and discussed.
Bringing the standards to life (2013)

Two rights standards - health and housing - were recognised by the Australian National Human Rights Consultation Committee (along with the right to education) as 'the primary economic and social rights... that are of the greatest concern to those who participated in all aspects of the [2009] Consultation', and the Committee recommended that they be included by the Federal Government in an interim list of rights to be protected and promoted immediately, as the question of how Australia should best recognise human rights at law is resolved. This recommendation has not been implemented to date.
Mining genomes and mapping proteomes: identification and characterization of protein subunit vaccines. (2000)

Currently, there is an extensive and unprecedented effort to obtain the complete nucleotide sequence of the complex genomes of many micro-organisms. In this post-genomic era, based on the availability of the entire genome sequence of an organism, three new disciplines of molecular biology have emerged: genomics, transcriptional profiling and proteomics. All these technologies have the potential to accelerate the process of identifying protective protein antigens as subunit vaccine targets as well as validating and extending the range of available candidate antigens. The progress of these technologies has led to the origination of the science of bioinformatics for management and critical evaluation of the large amount of information generated. Although genomics, transcriptional profiling and proteomics are each based on different principles, there is considerable synergy between them. Appropriate application of any one, or a combination of two or more of these approaches, coupled with bioinformatics, would allow identification of a short-list of vaccine candidates from the entire list of several hundreds to thousands of proteins encoded by the genome. These candidates would then require usual channelling through the subsequent process involving recombinant expression, purification and testing for immunogenicity and protective efficacy.
한국 직업변천사의 시대별 특성 비교분석에 관한 탐색적 연구 – 해방이후(1945) ~ 현재(2009)까지 – (2009)

본 연구는 해방이후 급격한 전개과정을 거쳐온 한국 직업변천사를 종합적·체계적으로 고찰, 분석하는 데 목적을 두고 있다. 해방이후 한국직업사는 각 시대의 산업환경과 전환기적 사건에 의해 명멸을 거듭해온 만큼 접근방법 또한 시대별 직업세계를 창출시킨 산업환경의 요인분석과 직업의 태생학적 메커니즘을 분석하는데 초점을 맞추었다. 해방이 후 70년간 전개되어온 한국의 직업변천사의 특징과 시사점을 요약하면 다음과 같다. 첫째, 한국의 직업변천사는 시대별 산업환경과 산업특성을 반영하고 있다. 둘째, 한국의 직업변천사와 산업변천사 간에는 상호 유기적 관계를 형성하고 있다. 셋째, 한국의 직업변천사는 전환기적 사건과 요인에 따라서 양태와 특징을 달리하고 있다. 넷째, 한국의 직업변천사는 시대별 산업구조와 고용구조를 읽을 수 있는 척도이다. 다섯째, 한국의 직업변천사는 시대별 직업인들의 속성과 직업의 사회적 입지를 나타내고 있다. 본 연구는 해방이후(1945) 현재(2009)까지 산업환경과 맞물려 전개과정을 거쳐온 직업세계를 시대별로 상호 비교분석하여 그 현황과 추이, 양태와 특징을 체계적으로 파악할 수 있도록 접근했다. 이는 해방이후 전개되어온 한국직업사를 시대별·산업별·직업별·특징별로 체계화시킨다는 의미와 더불어 향후 보다 발전된 직업세계와 직업문화를 유인하고 정착시키는 데 필요한 사적 직업자료와 직업정보를 동시에 구축해준다는 목적도 함께 내포하고 있다.
Deep Gesture Video Generation With Learning on Regions of Interest (2019)

Generating videos with semantic meaning, such as gestures in sign language, is a challenging problem. The model should not only learn to generate videos with realistic appearance, but also take notice of crucial details in frames to convey precise information. In this paper, we focus on the problem of generating long-term gesture videos containing precise and complete semantic meanings. We develop a novel architecture to learn the temporal and spatial transforms in regions of interest, i.e., gesticulating hands or face in our case. We adopt a hierarchical approach for generating gesture videos, by first making predictions on future pose configurations, and then using the encoder-decoder architecture to synthesize future frames based on the predicted pose structures. We develop the scheme of action progress in our architecture to represent how far the action has been performed during its expected execution, and to instruct our model to synthesize actions with various paces. Our approach is evaluated on two challenging datasets for the task of gesture video generation. Experimental results show that our method can produce gesture videos with more realistic appearance and precise meaning than the state-of-the-art video generation approaches.
In Vitro Performance of Floating Sustained-Release Capsule of Verapamil (1998)

Capsules filled with mixtures of verapamil, hydroxypropoxyl cellulose (HPC), and effervescent are proposed to provide floating sustained release over 10 hr. The effects of weight filled in the capsule, amount of HPC, and the addition of effervescent on the dissolution kinetics are studied. The conventional capsules were filled with different amounts and weights of the mixtures of verapamil, HPC, and effervescent. The release of verapamil from the capsules followed the Higuchi release model. However, when effervescent was added, a zero-order drug release was observed after the burst phase. The conventional capsule, when filled with active ingredients, polymers, and effervescent, can achieve a zero-order release system. Entrapped air was considered as a barrier to diffusion and matrix relaxation in drug release.
Thermotropic polyesters with non‐linear links (1980)

Abstract The use of different linking units to promote liquid crystal behaviour in rigid rod like polyarylates is reviewed. By systematically varying the proportion of non linear, meta, ortho , or bent bisphenol units in one particular series based on chloro‐ 1,4 phenylene: 4,4′ oxydibenzoate/terephthalate, complete series of compositions, extending from liquid crystal through to isotropic behaviour, have been prepared, characterised and compared. Using this data, parallels between thermotropic and lyotropic behaviour are illustrated and used to postulate physical models for the structure of liquid crystalline melts and solids.
Bax interacts with the permeability transition pore to induce permeability transition and cytochrome<i>c</i>release in isolated mitochondria (1998)

Cytochrome c release and the mitochondrial permeability transition (PT), including loss of the transmembrane potential (Deltapsi), play an important role in apoptosis. Using isolated mitochondria, we found that recombinant Bax and Bak, proapoptotic members of the Bcl-2 family, induced mitochondrial Deltapsi loss, swelling, and cytochrome c release. All of these changes were dependent on Ca2+ and were prevented by cyclosporin A (CsA) and bongkrekic acid, both of which close the PT pores (megachannels), indicating that Bax- and Bak-induced mitochondrial changes were mediated through the opening of these pores. Bax-induced mitochondrial changes were inhibited by recombinant Bcl-xL and transgene-derived Bcl-2, antiapoptotic members of the Bcl-2 family, as well as by oligomycin, suggesting a possible regulatory effect of F0F1-ATPase on Bax-induced mitochondrial changes. Proapoptotic Bax- and Bak-BH3 (Bcl-2 homology) peptides, but not a mutant BH3 peptide nor a mutant Bak lacking BH3, induced the mitochondrial changes, indicating an essential role of the BH3 region. A coimmunoprecipitation study revealed that Bax and Bak interacted with the voltage-dependent anion channel, which is a component of PT pores. Taken together, these findings suggest that proapoptotic Bcl-2 family proteins, including Bax and Bak, induce the mitochondrial PT and cytochrome c release by interacting with the PT pores.