gebhart/patrepeval huggingface dataset:
Expression of canine interferon‐β by a recombinant vaccinia virus (2000)

A recombinant vaccinia virus expressing canine interferon (IFN)-beta was constructed (vv/cIFN-beta). In rabbit kidney (RK13) and canine A72 cells infected with vv/cIFN-beta, the recombinant canine IFN-beta was detected in both cell extracts and supernatants, and the IFN activities of the culture supernatants were also detected. Inhibition of N-linked glycosylation by tunicamycin treatment indicated that the recombinant canine IFN-beta was modified by N-linked glycosylation in a different way between RK13 and A72 cells, and that N-linked glycosylation is essential for its secretion. The growth of vv/cIFN-beta at a low multiplicity of infection was inhibited by antiviral activity of canine IFN-beta, indicating that this recombinant virus could be used as a suicide viral vector.
Cloning and Characterization of Two K+ Inward Rectifier (Kir) 1.1 Potassium Channel Homologs from Human Kidney (Kir1.2 and Kir1.3) (1997)

The DNA sequence encoding the rat brain inward rectifier-10 K+ channel was amplified from rat brain RNA using reverse transcription-polymerase chain reaction and used to clone the human homolog. Low stringency screening of a human kidney cDNA library and subsequent DNA sequence analysis identified two related K+ inward rectifier cDNAs, referred to as Kir1.2 and Kir1.3, which were derived from transcription of distinct human genes. Kir1.2 represents the human homolog of the rat BIRK-10 sequence, whereas Kir1.3 was unique compared with all available sequence data bases. The genes that encode Kir1.2 and Kir1.3 were mapped to human chromosomes 1 and 21, respectively. Both genes showed tissue-specific expression when analyzed by Northern blots. Kir1.2 was only detected in brain >> kidney and was detected at high levels in all brain regions examined. Kir1.3 was most readily detected in kidney and was also expressed in pancreas > lung. Comparative analysis of the predicted amino acid sequences for Kir1.2 and Kir1.3 revealed they were 62% identical. The most remarkable difference between the two polypeptides is that the Walker Type A consensus binding motif present in both Kir1.1 and Kir1.2 was not conserved in the Kir1.3 sequence. Expression of the Kir1.2 polypeptide in Xenopus oocytes resulted in the synthesis of a K+-selective channel that exhibited an inwardly rectifying current-voltage relationship and was inhibited by external Ba2+ and Cs+. Kir1.2 current amplitude was reduced by >85% when the pH was decreased from pH 7.4 to 5.9 using the membrane-permeant buffer acetate but was relatively unaffected when pH was similarly lowered using membrane-impermeant biphthalate. The inhibition by intracellular protons was voltage-independent with an IC50 of pH 6.2 and a Hill coefficient of 1.9, suggesting the cooperative binding of 2 protons to the intracellular face of the channel. In contrast, Kir1.3 expression in Xenopus oocytes was not detectable despite the fact that the cRNA efficiently directed the synthesis of a polypeptide of the expected Mr in an in vitro translation system. Co-expression of Kir1.3 with either Kir1.1 or Kir1.2 reduced currents resulting from expression of these inward-rectifier subunits alone, consistent with a dominant negative influence on Kir1.1 and Kir1.2 expression.
Case 16-1962 (1962)

Presentation of CaseA twenty-five-year-old woman entered the hospital because of pain in the left flank.The patient was well until seven weeks before admission, when a "kidney infection" developed after a normal pregnancy and delivery. She complained of aching pain, which was localized to the left flank and exacerbated by lying down. The temperature rose once or twice daily, usually in association with chills. She did not respond to tetracycline, nitrofurantoin (Furadantin) and sulfisoxazole (Gantrisin), and intravenous pyelograms showed a cyst of the left kidney. She had a chronic, dry cough, without hemoptysis, for two months before admission and . . .
Chemoprevention in prostate cancer: The role of difluoromethylornithine (DFMO) (1992)

The polyamines are normal cell constituents considered to have an important role in the regulation of proliferation and differentiation. DFMO is an irreversible, enzyme-activated, suicide inhibitor of ornithine decarboxylase (ODC), the enzyme responsible for the first and rate-limiting step in mammalian polyamine synthesis. Preliminary data show that DFMO inhibits tumor cell growth in vitro and in vivo, and that it demonstrates chemopreventive activity in a variety of animal tumors. The prostate contains some of the highest concentrations of polyamines and of polyamine-synthetic enzymes (including ODC) in the mammalian organism. ODC activity in the prostate was shown to be more susceptible to DFMO inhibition than in other organs. We have found the ODC activity of the Dunning R3327 rat prostatic carcinomas to be as sensitive to inhibition by DFMO as the normal rat prostate. Furthermore, DFMO was inhibitory to the growth of the tumor both in vitro and in vivo. Given the slow growth rate and long latency period of human prostate cancer and the preliminary DFMO data, we suggest that clinical trials to evaluate the chemopreventive potential of DFMO in prostatic carcinoma deserve serious consideration.
Re-organization of the immunoglobulin kappa gene on both alleles is not an obligatory prerequisite for Ig lambda gene expression in human cells. (1991)

Re-arrangement and expression of the immunoglobulin (Ig) genes in B cells occurs under an ordered developmental control. The sequential model of Ig light chain exclusion predicts that only after non-productive re-organization or deletion of both Ig kappa alleles would re-arrangement of Ig lambda gene segments occur. To prove this model, we asked whether expression of Ig lambda light chains is always associated with rearrangement and/or deletion of both Ig kappa alleles in human cells. Therefore, we established human diploid B-cell clones in vitro that produce Ig lambda light chains. Southern blot analysis of the Ig kappa alleles revealed that three Ig lambda expressing cell lines (out of six Ig lambda+ cell lines tested) harbour one Ig kappa allele in germline configuration. Furthermore, a 1.5 kb RNA derived from the germline Ig kappa locus was detected by Northern blot hybridizations. The results implicate that the mechanism of Ig light chain exclusion is not precisely sequential and that it does not necessarily need re-arrangement or deletion of both Ig kappa alleles as a prerequisite for Ig lambda light chain expression in human cells.
Regression Analysis of Microwave Spectra for Temperature-Compensated and Density-Independent Determination of Wheat Moisture Content (1998)

Partial least-squares regression (PLSR) was used to generate wheat moisture content predictive models from eight-frequency microwave attenuation (A) and phase (P) spectra in the 10.36 to 18.0 GHz range, as obtained by a free-space technique with a 10.4 cm thick sample. Spectra ( n = 379) were measured for a set of grain samples that had been treated to span the agriculturally practical ranges of moisture content (M) (10.6 to 19.2% g/g wet ), temperature (K) (–1 to 42°C), and bulk density (D) (0.72 to 0.88 g/mL). The sample– property space formed by M, K, and D was used to prune redundant samples and select representative subsets for calibration ( n = 279), cross-validation ( n = 40 segments), and testing ( n = 31). Twelve model types are reported and vary from attenuation or phase alone to the combination of attenuation, phase, temperature, and density (i.e., APKD). For optimization of each PLSR model, the raw spectral, temperature, and density data were preprocessed with variable ratios, mathematical transformations, and/or variable scaling. The lowest moisture prediction errors were for temperature-and density-corrected models with variables AKD or APKD; these produced root-mean-square cross-validation and prediction errors (RMSECV and RMSEP) of 0.19 to 0.20% in moisture content units. The more practical unifrequency models, APK at 15.2 GHz, and AK at 18.0 GHz, yielded RMSECV values of 0.21% and 0.35%, respectively. Addition of temperature to dielectric data always substantially reduced the model error. However, the multiplicative effect of density is well corrected by using the ratio A/P, or partly corrected by using the features in the attenuation spectra. Data trends suggest that dual-frequency PK models might benefit from a wider frequency range, and unifrequency AK models might be better at frequencies higher than 18.0 GHz. The results presented make it possible to evaluate a wide variety of instrumental configurations that might be proposed to suit particular engineering criteria such as measurement accuracy, range of operating conditions, and hardware complexity.
An Analytical Approach To 2.5D Sound Field Reproduction Employing Circular Distributions Of Non-Omnidirectional Loudspeakers (2009)

Publication in the conference proceedings of EUSIPCO, Glasgow, Scotland, 2009
Athermal silicon waveguides with bridged subwavelength gratings for TE and TM polarizations (2012)

In this paper, athermal silicon waveguides using bridged subwavelength grating (BSWG) structures are proposed and investigated. The realization of temperature-independent BSWG waveguides for both polarizations is demonstrated numerically and experimentally. SU-8 polymer is used as the cladding material to compensate for the positive thermo-optic (TO) coefficient (dn/dT) of silicon. We investigate the dependence of the effective TO coefficient of BSWG waveguides on both the bridge width and grating duty cycle. The BSWG waveguides have a width of 490 nm, a height of 260 nm, and a grating pitch of 250 nm. Athermal behavior is achieved for both the transverse-magnetic (TM) and the transverse-electric (TE) polarized light for a variety of bridge width and duty cycle combinations. Furthermore, the BSWGs can be designed to be athermal for both TE and TM polarization simultaneously.
2‘-Ribose-Ferrocene Oligonucleotides for Electronic Detection of Nucleic Acids (2001)

We have synthesized two novel phosphoramidites with a ferrocenyl moiety at the 2'-ribose position linked through a butoxy linker. Using automated DNA/RNA synthesis techniques, oligonucleotides containing ferrocene at various positions were prepared and characterized by HPLC, MALDI-TOF mass spectrometry, and electrochemistry. Thermal stability studies of the ferrocene-modified DNA duplexes revealed that introduction of one or two ferrocenyl complexes does not result in an observed change of the T(m) values of the corresponding DNA duplexes when compared to the nonmodified hybrids. These data indicate that the introduction of a ferrocenyl group at the 2'-position of the ribose ring containing either a purine or pyrimidine base has no effect on the stability of the modified DNA. The electrochemical behavior of the ferrocene-containing DNA was examined by cyclic voltammetry. The modified 2'-ferrocene-oligonucleotides are electrochemically active and can be used as signaling probes for the electronic detection of nucleic acids on bioelectronic sensors.
Collaborative study procedures of the Association of Official Analytical Chemists (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCollaborative study procedures of the Association of Official Analytical ChemistsElwyn D. SchallCite this: Anal. Chem. 1978, 50, 3, 337A–340APublication Date (Print):March 1, 1978Publication History Published online21 September 2008Published inissue 1 March 1978https://pubs.acs.org/doi/10.1021/ac50025a003https://doi.org/10.1021/ac50025a003research-articleACS PublicationsRequest reuse permissionsArticle Views39Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optical Haar wavelet transforms using computer generated holography (1992)

This research introduces an optical implementation of the continuous wavelet transform to filter images. The wavelet transform is modeled as a correlation process and is implemented with a Vander Lugt correlator. The orthonormal basis set used is composed of two one-dimensional Haar functions and a two-dimensional Haar function. Each wavelet, as well as its first two dyadic scalings, is implemented with computer generated holography. The Interferogram method, which represents a function only in terms of its phase, is used to encode the wavelet functions. The results are imaged onto a CCD array and captured using a framegrabber. The optical results are compared to digital simulation. The results show that an optical implementation of the continuous wavelet transform was performed and that the results compared favorably to digital simulation. Wavelets, Holography, Optical correlators.
How Attractive is Bromine as a Protecting Group in Aromatic Chemistry? (2002)

The reversibility of electrophilic bromination of aromatic compounds has been known for a long time, but the possibility of using bromine as a protecting group in aromatic or heterocyclic chemistry has now been shown for the first time (see scheme for an example). Compared with typical protective groups (SO3H, COOH, NH2, CHMe2, CMe3) bromine offers many advantages; a decisive aspect for its successful application is the choice of optimized reaction conditions which are predictable from the reaction mechanism.
Line art rendering via a coverage of isoparametric curves (1995)

A line art nonphotorealistic rendering scheme of scenes composed of freeform surfaces is presented. A freeform surface coverage is constructed using a set of isoparametric curves. The density of the isoparametric curves is set to be a function of the illumination of the surface determined using a simple shading model, or of regions of special importance such as silhouettes. The outcome is one way of achieving an aesthetic and attractive line art rendering that employs isoparametric curve based drawings that is suitable for printing publication.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Probabilistic estimation of temporary lanes at road work zones (2012)

Road work zones pose a great challenge for the environmental perception with sensors and the correct modelling of the scenery. Especially the recognition of the valid lane is of great interest for driver assistance systems. At road work zones temporary lanes invalidate the original lanes while the latter are still present on the road. All lines are detected with a greyscale video camera. In this paper we present an approach which generates different hypotheses for the lanes originated from the two different types of lines. A particle filter is applied for smoothing and filtering while a JIPDA algorithm is used for association and with its existence estimation helps to find the most plausible hypothesis of the currently valid lane.
A Series of <i>Bis</i>(phosphinic)diamido Yttrium Complexes As Initiators for Lactide Polymerization (2008)

A series of new bis(phosphinic)diamido yttrium complexes have been synthesized and fully characterized. The complexes adopt dimeric structures, both in solution and in the solid state, where one phosphinic group bonds to one yttrium center and the other bonds to two yttrium centers. The complexes have all been tested as initiators for the ring-opening polymerization of lactide; they are all highly active. The rate of polymerization is controlled by the diamine backbone substituent with the rate depending on the backbone flexibility. The order of decreasing rates were 2,2-dimethyl-1,3-propylene > trans-1,2-cyclohexylene > 1,2-ethylene >> 1,2-phenylene. The polymerization kinetics showed, in most cases, an initiation period, during which the percentage conversion and the rate of polymerization were much lower than during propagation. This was attributed to relatively slow initiation by the bulky amido group. The initiator structure was probed using (31)P{ (1)H} NMR spectroscopy, which showed that the dimeric structure was maintained throughout the polymerization. The initiators give rise to controlled ring-opening polymerization as shown by the linear relationship between the percentage conversion and the number-average molecular weight.
Transient response of a peak voltage detector for sinusoidal signals (1992)

A fast peak detector for constant frequency sinusoidal signals is proposed. This detector is based on the concept of the orthogonal function set. The theoretical response time of this detector is less than one-fourth of a cycle. When this detector is used, the transient response of the output voltage is different under different inception angles. The mathematical equations to describe transient phenomena are derived and simulated. Because its transient response performance is superior to the conventional sinusoidal amplitude detector used in an analog controller, it can improve the transient response performance of the controller. For practical applications, the effects of harmonics and frequency variation are also discussed, simulated, and tested.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A 250 <italic>μ</italic>m × 57 <italic>μ</italic>m Microscale Opto-electronically Transduced Electrodes (MOTEs) for Neural Recording (2018)

Recording neural activity in live animals in vivo with minimal tissue damage is one of the major barriers to understanding the nervous system. This paper presents the technology for a tetherless opto-electronic neural interface based on 180 nm CMOS circuits, heterogeneously integrated with an AlGaAs diode that functions as both a photovoltaic and light emitting diode. These microscale opto-electrically transduced electrodes (MOTEs) are powered by and communicate through an optical interface, simultaneously enabling high temporal-resolution electrical measurements without a tether or a bulky RF coil. The MOTE presented here is 250 μm × 57 μm, consumes 1 μW of electrical power, and is capable of capturing and encoding neural signals before transmitting the encoded signals. The measured noise floor is as low as 15 μV<sub>RMS</sub> at a 15 kHz bandwidth.
Greenland Ice Sheet Elevation Change in Winter and Influence of Atmospheric Teleconnections in the Northern Hemisphere (2009)

在在冬季和海水平压力的格陵兰冰表(GIS ) 的表面举起的可变性之间的关系从忍受卫星的雷达高度表通过数据的分析被识别，和在 19932005 期间的气象学的数据域。我们发现那两个北方太平洋摆动(NPO ) 和北方大西洋摆动(NAO ) ，大气的表面的二个主要 teleconnection 模式在北半球迫使地，显著地影响 GIS 冬季举起变化。进一步， NPO 可以由影响 NAO 影响 GIS 累积，这被建议，特别地由改变紧张和地点冰岛低。
Brain specificity of diffuse optical imaging: improvements from superficial signal regression and tomography (2010)

Functional near infrared spectroscopy (fNIRS) is a portable monitor of cerebral hemodynamics with wide clinical potential. However, in fNIRS, the vascular signal from the brain is often obscured by vascular signals present in the scalp and skull. In this paper, we evaluate two methods for improving in vivo data from adult human subjects through the use of high-density diffuse optical tomography (DOT). First, we test whether we can extend superficial regression methods (which utilize the multiple source-detector pair separations) from sparse optode arrays to application with DOT imaging arrays. In order to accomplish this goal, we modify the method to remove physiological artifacts from deeper sampling channels using an average of shallow measurements. Second, DOT provides three-dimensional image reconstructions and should explicitly separate different tissue layers. We test whether DOT's depth-sectioning can completely remove superficial physiological artifacts. Herein, we assess improvements in signal quality and reproducibility due to these methods using a well-characterized visual paradigm and our high-density DOT system. Both approaches remove noise from the data, resulting in cleaner imaging and more consistent hemodynamic responses. Additionally, the two methods act synergistically, with greater improvements when the approaches are used together.
Oxygen Equilibrium and Kinetics of Isolated Subunits from Hemoglobin Kansas (1973)

The isolated beta subunit of hemoglobin Kansas has an oxygen affinity that is as low relative to the oxygen affinity of the beta(A) subunit as the affinity of hemoglobin Kansas is low relative to hemoglobin A. Thus the low affinity properties of hemoglobin Kansas are almost completely reflected in the properties of the isolated subunits. The kinetic results show that the equilibrium affinity difference results both from a much larger oxygen dissocation rate constant in beta(Kansas) (k = 37 sec(-1) and 18 sec(-1) for beta(Kansas) and beta(A), respectively) and from a lower association reaction rate, The properties of the alpha chains from hemoglobins A and Kansas appear to be identical, as expected.
Giant Bent-Core Mesogens in the Thread Forming Process of Marine Mussels (2004)

In marine mussels (Mytilus), byssal threads are made in minutes from prefabricated smectic polymer liquid crystals by a process resembling reaction injection molding. The mesogens in these arrays are known to be natural block copolymers with rodlike collagen cores. Using atomic force microscopy, it was shown that these collagenous mesogens are bent-core or banana-shaped in a manner that is consistent with and predictable from their amino acid sequence. The overall bend angle in preCOL-NG in Mytilus galloprovincialis is about 130°. The mesogens have a center-to-center separation of approximately 22 nm and a length of 200 nm. It is evident that the smectic structure of the prefabricated mesophases remains largely intact over 1−3 μm distances in the molded fibers and is presumably locked in place during molding by cross-linking. Like the smectic liquid crystals of many synthetic banana mesogens, the collagenous mesogens of the byssal threads exhibit SmC2 symmetry with a characteristic tilt of 24.6°. At about 100% extension, this tilt is considerably reduced and the globular end domains are no longer visible presumably because they have been unraveled.
Design, Evaluation, and Selection Of Heavy-Duty Rear Axles (1955)

<div class="section abstract"><div class="htmlview paragraph">HEAVY-DUTY rear axles are analyzed in this paper - the first L. Ray Buckendale Lecture to be delivered.</div><div class="htmlview paragraph">The author presents a practical method of evaluating heavy-duty rear axles, with emphasis on performance and axle ratio.</div><div class="htmlview paragraph">The major part of the paper is devoted to a discussion of the four functions of rear axles, as follows: (1) To carry the load, (2) to transmit driving torque, (3) to allow differential action, and (4) to help stop the vehicle.</div></div>