gebhart/patrepeval huggingface dataset:
Purification and specificity of lipases from<i>Geotrichum candidum</i> (1990)

A crude, commercialGeotrichum candidum lipase (EC 3.1.1.3) preparation (Amano GC-20) was purified by hydrophobic interaction chromatography on Octyl Sepharose. The purified enzyme is a microheterogeneous glycoprotein containing isozymes varying in molecular weight, pI and specificity. It consists of 64, 62 and 59 kDa species as determined by denaturing polyacrylamide gel electrophoresis. Five isozymes (pI 4.40, 4.47, 4.58, 4.67 and 4.72) are detected by isoelectric focusing using both silver and activity stains. Chromatofocusing was used to separate the isozymes according to pI. Although all the isozymes are specific for oleatevs stearate esters, one isozyme (pI 4.72) is also specific for oleatevs palmitate. The number of isozymes is reduced to two (pI 4.67 and 4.72) after carbohydrate removal using endoglycosidase F/N-glycosidase. These isozymes may be products of two lipase genes.
A Picosecond Resolution Time Digitizer for Laser Ranging (1978)

The Time Digitizer capable of covering a range of 0.34 sec in 9.76 psec increments is described. The time interval between a pair of start-stop pulses is digitized coarsely in 20 nsec periods by a very accurate 50 MHz reference clock. The residual fractions of a clock period at the start and the stop end of the measured interval are stretched in two interpolators and digitized in 9.76 psec increments. An equivalent digitizing frequency of 102.4 GHz is thus achieved. The digitizer is built in a CAMAC minicrate and communicates via a standard crate controller. It is intended for use in the laser ranging between ground stations and the Laser Geodetic Satellite (LAGEOS). It is shown that the distribution in any two adjacent 9.76 psec channels of a small number of identical test time intervals is essentially binomial. The performance of the digitizer and test results are given.
Cerebrospinal Fluid Neurochemical Phenotypes in Vascular Dementias: Original Data and Mini-Review (2008)

CSF neurochemical phenotypes sufficiently discriminate probable AD and VAD from each other, but their diagnostic value is limited in case of no clear-cut clinical appearance, such as possible VAD. Conversely, CSF Abeta peptides and p-tau levels may help estimate the involvement of AD-like pathophysiological pathways in VAD subgroups.
Modelling perfume deposition on fabric during a washing cycle: theoretical approach (2008)

Abstract The effect of surfactant micelles on the deposition of perfume molecules on fabric has been estimated theoretically for a full washing cycle, using mass transfer mechanisms. In the absence of a surfactant phase, the fraction of fragrance molecules deposited on fabric is well characterized by a sigmoidal curve with the logP value of the perfume molecules, which is a well‐known result. However, when surfactant is added at a concentration higher than the critical micellar concentration (CMC), high logP value molecules are lost, due to the hydrophobic phase created by the micelles. This phenomenon results in a modified sigmoidal behaviour of the fraction of fragrance molecules deposited, with the logP value that tends to reach the zero deposition for very high logP values. A maximum fraction deposited is located at intermediate logP values (between 1.5 and 3). Evidence of redeposition of high logP value molecules is shown when rinsing is considered. In this case, the micelles break if the overall surfactant concentration is below the CMC, and the trapped perfume molecules are released and are able to deposit on the fabric. This model is successfully subjected to measured performance of the key raw perfume materials. Copyright © 2008 John Wiley & Sons, Ltd.
Fly Ash Penetration through Electrostatic Precipitator and Flue Gas Condenser in a 6 MW Biomass Fired Boiler (2002)

The effects of an electrostatic precipitator and a flue gas condenser on size resolved fly ash particle concentration and composition were studied in a 6 MW biomass combustion unit, fired with moist forest residue. The boiler was of moving grate type. The fly ash particles were sampled upstream and downstream of the electrostatic precipitator and flue gas condenser, respectively. Fine particle number size distributions were measured using an electric mobility spectrometer (SMPS) and coarse particle number size distributions were measured using a time-of-flight instrument (APS). The mass size distributions were measured using a multi-jet low pressure cascade impactor (DLPI). For chemical analyses of the impactor substrates particle induced X-ray emission analysis (PIXE) was used. After the flue gas passed the electrostatic precipitator (ESP), the fly ash particle concentration was reduced by approximately 96% by number and 83% by mass. After the particles passed the flue gas condenser, particle number concentration was only marginally altered, whereas the mass concentration was reduced by half. Both the ESP and the condenser showed, size dependent particle separation efficiency. The main elements (Z > 12) in the fine fly ash fraction were K, S, and Cl, whereas the main elements in the coarse fraction were Ca, K, S, and Cl. After passing the ESP the mass ratio of Ca decreased in the coarse fraction, while the ratios of K, S, and Cl increased, indicating transference of fly ash material from the fine to the coarse particle fraction. There was no significant difference in the elemental composition for any particle size fraction sampled upstream or downstream of the condenser.
Fairness and Accountability Design Needs for Algorithmic Support in High-Stakes Public Sector Decision-Making (2018)

Calls for heightened consideration of fairness and accountability in algorithmically-informed public decisions-like taxation, justice, and child protection-are now commonplace. How might designers support such human values? We interviewed 27 public sector machine learning practitioners across 5 OECD countries regarding challenges understanding and imbuing public values into their work. The results suggest a disconnect between organisational and institutional realities, constraints and needs, and those addressed by current research into usable, transparent and 'discrimination-aware' machine learning-absences likely to undermine practical initiatives unless addressed. We see design opportunities in this disconnect, such as in supporting the tracking of concept drift in secondary data sources, and in building usable transparency tools to identify risks and incorporate domain knowledge, aimed both at managers and at the 'street-level bureaucrats' on the frontlines of public service. We conclude by outlining ethical challenges and future directions for collaboration in these high-stakes applications.
Macrophage Targeted <i>N</i>-(2-Hydroxypropyl)methacrylamide Conjugates for Magnetic Resonance Imaging (2006)

This study describes the synthesis, characterization and in vitro evaluation of targetable N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-gadolinium (Gd) chelates for enhanced magnetic resonance imaging (MRI) of macrophages. Copolymers of HPMA, methacryloylglycylglycyl-mannosamine (MA-GG-ManN), aminopropylmethacrylamide-benzyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (APMA-DOTA), and 5-(3-(methacryloylaminopropyl)thioureidyl) fluorescein (MA-AP-FITC) were synthesized and characterized. Gd was chelated to the polymeric precursors. The conjugates were characterized for gadolinium content by inductively coupled plasma optical emission spectrometry (ICP-OES) and T1 relaxivity (r1) at room temperature and 1.5 T. The effect of ManN content on mannose receptor mediated uptake of THP-1 human macrophages was evaluated as a function of time and temperature. The polymer conjugates showed relaxivities in the range of 21.8-24.9 s(-1) mM(-1) Gd. Relaxivities of the conjugates per mM Gd were up to 7 times higher than that of a commercially available MR contrast agent Gd-DOTA. Significantly (p < 0.042) higher uptake was observed for targeted conjugates compared to nontargeted conjugates. The uptake of polymeric conjugates was time and concentration dependent and appears to be mannose receptor mediated. The increased relaxivity coupled with the ability to target these carriers to cells containing ManN receptors shows promise for the application of these agents in clinical MR imaging of macrophage mediated malignancies.
The effect of preweaning and postweaning housing on the behaviour of the laboratory mouse (<i>Mus musculus</i>) (2007)

Standard housing for laboratory mice severely restricts natural behaviour and the control that the animal has over its environment. Providing the cage with objects is a method that has been used to both increase environmental complexity, promote the performance of natural behaviour and provide greater controllability for the animal. This method of furnishing cages has mostly been studied in adult animals, and little is known about the influence that the preweaning environment has on the behaviour of mice as adults. This study aimed to investigate the effects on mice behaviour of preweaning and postweaning housing environment. In this experiment, 64 pairs of animals of the strain C57BL/6J were used. Half of the animals were born and reared until weaning in standard cages and the other half in cages twice the size of the standard and furnished with nesting material, a cardboard tube, a PVC nest box and a wooden chewblock. After weaning, half the animals in each group were changed to the other type of cage, whereas the other half remained in the same environment; in both cases they were kept in single-sex pairs of littermates. Behaviour during the dark, active period was studied through video recordings. We found no main effects of preweaning environment on behaviour; however, mice moved from furnished to standard cages at weaning showed a decrease in inactive behaviour at four weeks of age. Mice housed after weaning in standard cages spent less time inactive, and more time engaging in activities like feeding and drinking, self-grooming and allogrooming. A sex difference was also found, in that females showed a greater performance of exploratory behaviour as well as a greater prevalence of stereotypies. The use of different objects and locations within the furnished cage was also analysed at both ages. Results show that at eight weeks of age mice spent more time at the top of the cage, and that the use of the nest box (although not for resting) increased between four and eight weeks. Mice were found to use the nest box as a nesting site/sleeping place only at age four weeks, whereas they always used the nesting material for sleeping.
An Improved Film Cryotron and Its Application to Digital Computers (1960)

A crossed film cryotron deposited on an insulated superconductor is described and analyzed. It has a time constant of less than one μsec and is approximately 100 times faster than the original crossed film cryotron [4]. The dc dissipation is less than five μw and the active area of each element is approximately 5×10-4 cm2. These cryotrons and all their interconnecting circuitry can be deposited at one and the same time in a few simple steps. A cryotron storage circuit and a shift register is described, based upon a principle unique to superconductors. The shift register shown is deposited in an area corresponding to 18,000 active elements per square foot. Calculations are presented to show that with this component density, a computer or memory containing more than one million elements can be operated in a one-cubic-foot container using a one-watt output liquid helium refrigerator.
Antiviral Activities of Methylated Nordihydroguaiaretic Acids. 2. Targeting Herpes Simplex Virus Replication by the Mutation Insensitive Transcription Inhibitor Tetra-<i>O</i>-methyl-NDGA (1998)

We had previously reported that tetramethyl-O-NGDA (M4N), a synthetic derivative of the naturally occurring nordihydroguaiaretic acid (NDGA), is able to inhibit HIV Tat transactivation by blocking host Sp1 protein at the Sp1 cognate binding site on the HIV LTR promoter. The present studies were undertaken to examine whether M4N is able to inhibit the replication of herpes simplex virus (HSV), another Sp1-regulated virus. The results showed that in Vero cells, M4N inhibits at micromolar levels (IC50 = 43.5 microM) the expression of the herpes immediate early gene (alpha-ICP4), which is essential for HSV replication. An electrophoretic mobility shift assay, examining Sp1 binding to the alpha-ICP4 promoter, showed a significant inhibition of the control bands: 88% inhibition of the fast moving band (FMB) and 45% of the slow moving band (SMB), at 100 microM of drug concentration. Comparative studies between M4N and acycloguanosine (acyclovir, ACV) in cultured Vero cells revealed an interesting pattern in the drug sensitivity (IC50) and cytotoxicity (TC50) parameters. For M4N, the IC50 varied between 11.7 and 4 microM in 10 passages of HSV-1 and 4 passages of HSV-2 with no indication for a requirement of higher drug concentration. In contrast, for acyclovir, the IC50 increased from 7 microM in the first passage to 444 microM in the tenth passage of HSV-1, and >88 microM for the fourth passage of HSV-2, indicating a rapid build-up of drug resistance against acyclovir. While the selective index (SI), defined as the ratio: TC50/IC50, remained relatively constant for M4N; it dropped 60-fold for acyclovir in the endpoints of viral passages. Drug sensitivity for M4N toward the acyclovir-sensitive strain (sm44) and the acyclovir-resistant strain (ACV-10) of HSV-1 was similar, indicating no cross-resistance between M4N and acyclovir in their anti-HSV effects. These results may have an important clinical relevance since HSV has been shown to be a factor for spreading of HIV.
Ultrasound data acquisition system design for collecting high quality RF data from beef carcasses in the slaughterhouse environment (2003)

Ultrasound has considerable potential to accurately and precisely grade beef. The ultimate goal is an automated and objective grading process. The current phase of the work does not involve construction of an ultrasound grading device but rather the determination of which of these ultrasound-related and derived quantities best correlate to each grade. In order to make this determination, an ultrasound data acquisition system capable of acquiring large amounts of high-quality RF data from beef carcasses has been constructed. The engineering concepts and construction details of an ultrasound data acquisition device to be used as a research tool for this application are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Colostrinine: a proline-rich polypeptide from ovine colostrum is a modest cytokine inducer in human leukocytes. (1996)

A proline-rich polypeptide (PRP), now named colostrinine, molecular weight 18,000, was isolated from ovine colostrum and characterized by Janusz, Lisowski et al. The nonapeptide (NP) which is an active fragment of PRP was obtained by chemical synthesis. In mice, PRP has many regulatory effects on the humoral and cellular immune response. The present paper describes PRP as a cytokine inducer. PRP at concentration of 1-100 micrograms/ml was found to induce production of interferon (IFN) and tumor necrosis factor (TNF) in human peripheral blood leukocytes and in whole blood cultures. The effects were dose related. The identified till now cytokines induced by PRP were IFN-gamma and TNF-alpha but many other cytokines may be stimulated also. NP was considerably less active as the cytokine inducer than the natural PRP. Two volunteers given orally once daily for two to three weeks 100 or 200 micrograms PRP in tablets were found to develop the tolerance of IFN induction and had the modified TNF response. Furthermore, the PRP-treated volunteers showed signs of psycho-stimulation. Taken together our observations suggest that ovine PRP is active in humans and may have therapeutic value as an immunostimulant and/or neurotropic cytokine.
Dengue fever and dengue haemorrhagic fever in adolescents and adults (2012)

Dengue fever (DF) is endemic in tropical and subtropical zones and the prevalence is increasing across South-east Asia, Africa, the Western Pacific and the Americas. In recent years, the spread of unplanned urbanisation, with associated substandard housing, overcrowding and deterioration in water, sewage and waste management systems, has created ideal conditions for increased transmission of the dengue virus in tropical urban centres. While dengue infection has traditionally been considered a paediatric disease, the age distribution of dengue has been rising and more cases have been observed in adolescents and adults. Furthermore, the development of tourism in the tropics has led to an increase in the number of tourists who become infected, most of whom are adults. Symptoms and risk factors for dengue haemorrhagic fever (DHF) and severe dengue differ between children and adults, with co-morbidities and incidence in more elderly patients associated with greater risk of mortality. Treatment options for DF and DHF in adults, as for children, centre round fluid replacement (either orally or intravenously, depending on severity) and antipyretics. Further data are needed on the optimal treatment of adult patients.
Pain, 86 (2000) 321-322 Pain 4202 (2000)

Pain Research, Nuffield Department of Anaesthetics, University of Oxford, The Churchill, Oxford Radcliffe Hospital, Headington, Oxford OX3 7LJ, UK
Antinuclear Antibodies in Diagnosis and Management (1983)

Antinuclear antibodies are known to be present in a variety of autoimmune diseases, and they appear to play a primary role in the pathogenesis of a subgroup of "ANA diseases." It has been shown that each presents a distinct ANA profile, characterized by the presence and titer of certain antibodies and the absence of others. These profiles are of practical value in diagnosis.
Conflict-free Replicated Data Types (2011)

Replicating data under Eventual Consistency (EC) allows any replica to accept updates without remote synchronisation. This ensures performance and scalability in large-scale distributed systems (e.g., clouds). However, published EC approaches are ad-hoc and error-prone. Under a formal Strong Eventual Consistency (SEC) model, we study sufficient conditions for convergence. A data type that satisfies these conditions is called a Conflict-free Replicated Data Type (CRDT). Replicas of any CRDT are guaranteed to converge in a self-stabilising manner, despite any number of failures. This paper formalises two popular approaches (state- and operation-based) and their relevant sufficient conditions. We study a number of useful CRDTs, such as sets with clean semantics, supporting both add and remove operations, and consider in depth the more complex Graph data type. CRDT types can be composed to develop large-scale distributed applications, and have interesting theoretical properties.
Visual and Eye Movement Functions of the Posterior Parietal Cortex (1989)

Lesions of the posterior parietal area in humans produce interesting spatial-perceptual and spatial-behavioral deficits. Among the more important deficits observed are loss of spatial memories, problems representing spatial relations in models or drawings, disturbances in the spatial distribution of attention, and the inability to localize visual targets. Posterior parietal lesions in nonhuman primates also produce visual spatial deficits not unlike those found in humans. Mountcastle and his colleagues were the first to explore this area, using single cell recording techniques in behaving monkeys over 13 years ago. Subsequent work by Mountcastle, Lynch and colleagues, Hyvarinen and colleagues, Robinson, Goldberg & Stanton, and Sakata and colleagues during the period of the late 1970s and early 1980s provided an informational and conceptual foundation for exploration of this fascinating area of the brain. Four new directions of research that are presently being explored from this foundation are reviewed in this article. \n\n1. The anatomical and functional organization of the inferior parietal lobule is presently being investigated with neuroanatomical tracing and single cell recording techniques. This area is now known to be comprised of at least four separate cortical fields. \n\n2. Neural mechanisms for spatial constancy are being explored. In area 7a information about eye position is found to be integrated with visual inputs to produce representations of visual space that are head-centered (the meaning of a head-centered coordinate system is explained on p. 13).\n3. The role of the posterior parietal cortex, and the pathways projecting into this region, in processing information about motion in the visual world is under investigation. Visual areas within the posterior parietal cortex may play a role in extracting higher level motion information including the perception of structure-from-motion. \n\n4. A previously unexplored area within the intraparietal sulcus has been found whose cells hold a representation in memory of planned eye movements. Special experimental protocols have shown that these cells code the direction and amplitude of intended movements in motor coordinates and suggest that this area plays a role in motor planning.
Induction of isotype switching and Ig production by CD5+ and CD10+ human fetal B cells (1992)

In the present study the capacity of early fetal B cells to produce Ig was investigated. It is shown that B cells from fetal liver, spleen, and bone marrow (BM) can be induced to produce IgM, IgG, IgG4, and IgE, but not IgA, in response to IL-4 in the presence of anti-CD40 mAb or cloned CD4+ T cells. Even splenic B cells from a human fetus of only 12 wk of gestation produced these Ig isotypes. IFN-alpha, IFN-gamma, and transforming growth factor-beta inhibited IL-4-induced IgE production in fetal B cells, as described for mature B cells. The majority of B cells in fetal spleen expressed CD5 and CD10 and greater than 99% of B cells in fetal BM were CD10+. Highly purified CD10+, CD19+ immature B cells and CD5+, CD19+ B cells could be induced to produce Ig, including IgG4 and IgE, in similar amounts as unseparated CD19+ B cells. Virtually all CD19+ cells still expressed CD10 after 12 days of culture. However, the IgE-producing cells at the end of the culture period were found in the CD19-,CD10- cell population, suggesting differentiation of CD19+,CD10+ B cells into CD19-,CD10- plasma cells. Pre-B cells are characterized by their lack of expression of surface IgM (sIgM). Only 30 to 40% of BM B cells expressed sIgM. However, in contrast to sIgM+,CD10+,CD19+ immature B cells, sorted sIgM-,CD10+,CD19+ pre-B cells failed to differentiate into Ig-secreting cells under the present culture conditions. Addition of IL-6 to these cultures was ineffective. Taken together, these results indicate that fetal CD5+ and CD10+ B cells are mature in their capacity to be induced to Ig isotype switching in vitro as soon as they express sIgM.