gebhart/patrepeval huggingface dataset:
Planificacion de trayectorias para sistemas multi robot en entornos dinamicos (2001)

Esta tesis presenta algunas aportaciones a la planificacion de trayectorias para robots articulados. El trabajo se centra en la planificacion en entornos dinamicos y sometidos a incertidumbres y esta orientado a sistemas de varios manipuladores roboticos. Los metodos de planificacion de trayectorias para robots articulados se pueden clasificar en dos grandes grupos: metodos basados en informacion global, completos, pero computacionalmente costosos y los basados en informacion local, rapidos y reactivos, pro excesivamente cortos de vista. En los ultimos anos se han desarrllado metodos de planificacion basados en informacion global capaces de responder a los problemas de robots de tipo industrial. Estos metodos quedan, sin embargo , reducidos a entornos estaticos y donde no existen incertidumbres. La presente tesis expone un numero metodo de planificacion de trayectorias para manipuladores roboticos en entornos dinamicos que pretende superar las carencias de los metodos puramente locales o globales. El metodo se basa en dos ideas. La primera de ellas es restringir los movimientos del robot a un subespacio de pocas dimensiones del espacio de configuracion. De esta forma la planificacion de trayectorias reduce su complejidad y puede realizarse una planificacion rectiva en linea, que permite al robot moverse en entornos dinamicos y donde la trayectoria de los obstaculos no es completamente conocida. La segunda idea consiste en realizar una etapa de analisis fuera de linea para elegir de forma conveniente el subespacio mencionado. El metodo se ha aplicado a un sistema multi robot compuesto por tres manipuladores tipo puma. Los experimentos se han realizado tanto sobre simulaciones del sistema como sobre el equipo de laboratorio.
Biophysical basis, stability, and directional response characteristics of multiplicative lateral inhibitory neural networks (1989)

Summary form only given, as follows. A directionally selective nonlinear lateral inhibitory neural network is proposed. The network originates from the biophysical mechanism of shunting inhibition. Its stability and some of its directional response characteristics are examined. The most significant property of this network is its differential response to stimuli moving in opposite directions. This directional response is found to vary with the mean input strength level, the size and speed of moving objects, as well as with coupling among elements of the network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Tumour necrosis factor (TNF) gene polymorphism influences TNF-α production in lipopolysaccharide (LPS)-stimulated whole blood cell culture in healthy humans (1998)

TNF-alpha is involved in infectious and immuno-inflammatory diseases. Different individuals may have different capacities for TNF-alpha production. This might determine a predisposition to develop some complications or phenotypes of these diseases. The aims of our study were to assess the inter-individual variability of TNF-alpha production and to correlate this variability to a single base pair polymorphism located at position -308 in TNF gene. We studied 62 healthy individuals. TNF-alpha production after LPS stimulation was evaluated using a whole blood cell culture model. The TNF gene polymorphism was studied by an allele-specific polymerase chain reaction. Other cytokines produced in the culture, soluble CD14 concentrations and expression of CD14 on blood cells were also measured. Among the 62 individuals, 57 were successfully genotyped. There were 41 TNF1 homozygotes and 16 TNF1/TNF2 heterozygotes. TNF-alpha production after LPS stimulation of whole blood cell culture was higher among TNF2 carriers than among TNFI homozygotes (929pg/ml (480-1473pg/ml) versus 521 pg/ ml (178-1307 pg/ml); P<0.05). This difference was even more significant after correction of TNF-alpha production for CD14 expression on blood cells. In conclusion, the single base pair polymorphism at position -308 in the TNF gene may influence TNF-alpha production in healthy individuals.
GaN:ZnO Solid Solution as a Photocatalyst for Visible-Light-Driven Overall Water Splitting (2005)

Photocatalytic overall water splitting has been studied extensively from the viewpoint of solar energy conversion. Despite numerous attempts, none have yielded satisfactory results for the development of photocatalysts, which work under visible light irradiation to efficiently utilize solar energy. We report here the first example of visible-light-driven overall water splitting on a novel oxynitride photocatalyst, a solid solution of GaN and ZnO with a band gap of 2.58-2.76 eV, modified with RuO2 nanoparticles. In contrast to the conventional non-oxide photocatalysts, such as CdS, the solid solution is stable during the overall water splitting reaction. This is the first example of achieving overall water splitting by a photocatalyst with a band gap in the visible light region, which opens the possibility of new non-oxide-type photocatalysts for energy conversion.
Chk2/hCds1 functions as a DNA damage checkpoint in G<sub>1</sub>by stabilizing p53 (2000)

Chk2/hcds1, the human homolog of the Saccharomyces cerevisiae RAD53/SPK1 and Schizosaccharomyces pombe cds1 DNA damage checkpoint genes, encodes a protein kinase that is post-translationally modified after DNA damage. Like its yeast homologs, the Chk2/hCds1 protein phosphorylates Cdc25C in vitro, suggesting that it arrests cells in G(2) in response to DNA damage. We expressed Chk2/hCds1 in human cells and analyzed their cell cycle profile. Wild-type, but not catalytically inactive, Chk2/hCds1 led to G(1) arrest after DNA damage. The arrest was inhibited by cotransfection of a dominant-negative p53 mutant, indicating that Chk2/hCds1 acted upstream of p53. In vitro, Chk2/hCds1 phosphorylated p53 on Ser-20 and dissociated preformed complexes of p53 with Mdm2, a protein that targets p53 for degradation. In vivo, ectopic expression of wild-type Chk2/hCds1 led to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2/hCds1 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization. Thus, in response to DNA damage, Chk2/hCds1 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G(1).
Photopatterned oil-reservoir micromodels with tailored wetting properties (2015)

Micromodels with a simplified porous network that represents geological porous media have been used as experimental test beds for multiphase flow studies in the petroleum industry. We present a new method to fabricate reservoir micromodels with heterogeneous wetting properties. Photopatterned, copolymerized microstructures were fabricated in a bottom-up manner. The use of rationally designed copolymers allowed us to tailor the wetting behavior (oleophilic/phobic) of the structures without requiring additional surface modifications. Using this approach, two separate techniques of constructing microstructures and tailoring their wetting behavior are combined in a simple, single-step ultraviolet lithography process. This microstructuring method is fast, economical, and versatile compared with previous fabrication methods used for multi-phase micromodel experiments. The wetting behaviors of the copolymerized microstructures were quantified and demonstrative oil/water immiscible displacement experiments were conducted.
Decoupling Energy Storage from Electrochemical Reactions in Li–Air Batteries toward Achieving Continuous Discharge (2017)

Intrinsic performance issues in Li–air batteries, such as poor reversibility and power, result from multiple functions at the cathode. Electrochemical reactions and discharge product storage are two such contradictory functions that interfere with each other. Here, we propose a new concept Li–air battery that can decouple those functions via simple yet drastic changes in the electrolyte composition, completely eliminating Li+ ions in the area around the cathode using an ionic liquid. The Li+-free environment for the cathode reaction realizes continuous discharge, in similar fashion to fuel cells, for more than 1000 h due to its unique concept of storing generated Li2O2 in the bulk electrolyte. The new concept opens an avenue to redesign Li–air battery systems with wide flexibility to balance power and capacity independently and efficiently.
Application of motors to household refrigeration compressors (1952)

Modern refrigeration demands a motor-compressor unit of long life, which will operate over long periods with little or no attention with high efficiency. To achieve this end, the designer of the hermetically sealed motors used today must take into consideration a number of factors peculiar to this application.
Further characterization of HeLa S3 plasma membrane ghosts (1978)

A plasma membrane fraction of HeLa S3 cells, consisting of ghosts, is characterized more fully. A simple procedure is described which permits light and electron microscope study of the plasma membrane fraction through the entire depth of the final product pellet and through large areas parallel to the surface. Contamination by nuclei is 0.14%, too little for DNA detection by the diphenylamine reaction. Contamination by rough endoplasmic reticulum and ribosomes is small, a single ghost containing about 3% of the RNA in a single cell. Mitochondria were not encountered. Electron microscopy also shows (a) small vesicles associated with the outer surface of the ghosts, and (b) a filamentous web at the inner face of the ghost membrane. Sodium dodecyl sulfate (SDS)-polyacrylamide gel analysis shows that of the many Coomassie Blue-stained bands two were prominent. One, 43,000 daltons, co-migrated with purified rabbit muscle actin and constituted about 7.5% of the plasma membrane protein. The other major band, 34,000 daltons, was concentrated in the plasma membrane fraction. Two major glycoproteins detected by autoradiography of [14C]fucose-labeled glycoproteins on the gels, had apparent molecular weights of 35,000 daltons and 32,000 daltons. These major bands did not stain with Coomassie Blue. There were many other minor glycoprotein bands in the 200,000- to 80,000-dalton range. Ouabain-sensitive, Na+, K+-adenosine triphosphatase (ATPase) activity of the ghost fraction is purified 9.1 (+/- 2.2) times over the homogenate; recover of the activity is 12.0 (+/- 3.8%) of the homogenate. Enrichment and recovery of fucosylglycoprotein parallel those for ouabain-sensitive Na+, K+-ATPase activity. Fucosyl glycoprotein is recovered more than the enzyme activity in a smooth membrane vesicle fraction probably containing the bulk of plasma membrane not recovered as ghosts.
An integrated workflow for robust alignment and simplified quantitative analysis of NMR spectrometry data (2011)

The workflow performance was evaluated using a previously published dataset. Correlation maps, spectral and grey scale plots show clear improvements in comparison to other methods, and the down-to-earth quantitative analysis works well for the CluPA-aligned spectra. The whole workflow is embedded into a modular and statistically sound framework that is implemented as an R package called "speaq" ("spectrum alignment and quantitation"), which is freely available from http://code.google.com/p/speaq/.
Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli (2001)

In order to facilitate the large-scale preparation of active class II polyhydroxyalkanoate (PHA) synthase, we constructed a vector pT7-7 derivative that contains a modified phaC1 gene encoding a PHA synthase from Pseudomonas aeruginosa possessing six N-terminally fused histidine residues. Overexpression of this phaC1 gene under control of the strong Ø10 promoter was achieved in Escherichia coli BL21(DE3). The fusion protein was deposited as inactive inclusion bodies in recombinant E. coli, and contributed approx. 30% of total protein. The inclusion bodies were purified by selective solubilization, resulting in approx. 70–80% pure PHA synthase, then dissolved and denatured by 6M guanidine hydrochloride. The denatured PHA synthase was reversibly immobilized on a Ni2+-nitrilotriacetate–agarose matrix. The matrix-bound fusion protein was refolded by gradual removal of the chaotropic reagent. This procedure avoided the aggregation of folding intermediates which often decreases the efficiency of refolding experiments. Finally, the refolded fusion protein was eluted with imidazole. The purified and refolded PHA synthase protein showed a specific enzyme activity of 10.8m-units/mg employing (R/S)-3-hydroxydecanoyl-CoA as substrate, which corresponds to 27% of the maximum specific activity of the native enzyme. The refolding of the enzyme was confirmed by CD spectroscopy. Deconvolution of the spectrum resulted in the following secondary structure prediction: 10% α-helix, 50% β-sheet and 40% random coil. Gel filtration chromatography indicated an apparent molecular mass of 69kDa for the refolded PHA synthase. However, light-scattering analysis of a 10-fold concentrated sample indicated a molecular mass of 128kDa. These data suggest that the class II PHA synthase is present in an equilibrium of monomer and dimer.
Molecular approaches to receptors as targets for drug discovery (2000)

Many receptors have been selected as viable drug discovery targets. One particular class of receptors that have received much interest and so far relatively good success are the receptor protein tyrosine kinases (RPTKs). Typically, RPTKs are activated following the binding of the peptide growth factor ligand to its receptor. The RPTKs play crucial roles in signal transduction pathways that regulate a number of cellular functions, such as cell differentiation and proliferation, both under normal physiological conditions as well as in a variety of pathological disorders. A variety of different tumour types have been shown to have dysfunctional RPTKs, either as a result of excess production of the growth factor, the receptor or both, or via mutations in the RPTKs structure. Irrespective of the cause, this leads to the over-activity of the particular RPTK system and in turn to the aberrant and inappropriate cellular signalling within the tumour cell. RPTKs are attractive targets in the search for therapeutic agents, not only against cancers but also against many other disease indications. Although an ever-increasing number of RPTKs have been selected as viable molecular targets for drug discovery programmes, four examples will be covered in this article. These are the epidermal growth factor receptor (EGF-R), platelet-derived growth factor receptor (PDGF-R), fibroblast growth factor receptor (FGR-R) and vascular endothelial growth factor receptor (VEGF-R), with the main emphasis of interest being on their role in oncology.
Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. (1965)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Synthesis and Conformation of Poly(<scp>l</scp>-2-anthraquinonylalanine) (1997)

A novel nonnatural amino acid that carries a 2-anthraquinonyl group, l-2-anthraquinonylalanine, was first synthesized and converted to the corresponding N-carboxyanhydride (NCA). The NCA was polymerized to give poly(l-2-anthraquinonylalanine) in the form of a diblock copolymer with poly(ethylene glycol) that is linked to the C-terminal. A triblock copolymer was also prepared with poly(γ-benzyl l-glutamate)s that are attached to both N- and C-terminals. CD spectra of the diblock copolymer showed a helical conformation that is different from a right-handed α-helix. Conformational analysis and theoretical CD calculation suggested that the poly(anthraquinonylalanine) unit prefers a left-handed α-helix with the lowest energy side-chain orientation.
Molecular dielectrophoresis of bio-polymers (2003)

Dielectrophoresis (DEP) has found wide applications in manipulations of micron-sized particles such as cells. The authors used a fluid integrated circuit (FIC) concept to fabricate micron-sized electrodes, with which a very high intensity high-frequency field, over 1*10/sup 6/ V/m at 1 kHz to 10 MHz, was created in the steady state. The observation of molecular DEP using Avidin and other biopolymers showed that DEP occurs at field strengths of 0.4-1.0*10/sup 6/ V/m, which is substantially lower than DEP theory predicts. As applications of the molecular DEP, a molecular patterning method and dielectrophoretic chromatography are proposed. In the latter, a biopolymer solution is allowed to flow through a high-intensity field region created in a FIC, and the difference in DEP attraction is used to analyze and/or separate the polymer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Demonstration of quantum synchronization based on second-order quantum coherence of entangled photons (2016)

Based on the second-order quantum interference between frequency entangled photons that are generated by parametric down conversion, a quantum strategic algorithm for synchronizing two spatially separated clocks has been recently presented. In the reference frame of a Hong-Ou-Mandel (HOM) interferometer, photon correlations are used to define simultaneous events. Once the HOM interferometer is balanced by use of an adjustable optical delay in one arm, arrival times of simulta- neously generated photons are recorded by each clock. The clock offset is determined by correlation measurement of the recorded arrival times. Utilizing this algorithm, we demonstrate a proof-of-principle experiment for synchronizing two clocks separated by 4 km fiber link. A minimum timing stability of 0.44 ps at averaging time of 16000 s is achieved with an absolute time accuracy of 73.2 ps. The timing stability is verified to be limited by the correlation measurement device and ideally can be better than 10 fs. Such results shine a light to the application of quantum clock synchronization in the real high-accuracy timing system.
The Effect of Different Lubrication Methods on the Performance of a Tilting-Pad Journal Bearing (1991)

This paper contains the results of comparisons between the effects of three different lubrication methods, flooded, directed and leading-edge, on the performance of a tilting-pad journal bearing. Experimental pad temperatures and power loss data are presented for a 127 mm diameter, four pad bearing, operating over a range of shaft speeds up to 7000 rpm (46.6 m/s) and loads up to 22 kN (1.3 MPa). The experimental data indicate that temperature and power loss reductions are attainable with the use of the different lubrication methods. However, these are not as significant as those achieved with their use in tilting-pad thrust bearings.
A soft magnetic underlayer with negative uniaxial magnetocrystalline anisotropy for suppression of spike noise and wide adjacent track erasure in perpendicular recording media (2006)

The suppression of spike noise and wide adjacent track erasure (WATE) are important technical issues in the development of a perpendicular recording medium (PRM). As a solution to both of these problems, this paper presents a type of soft magnetic underlayer (SUL) with negative uniaxial perpendicular magnetic anisotropy. The magnetic anisotropy is achieved by employing a material with negative uniaxial magnetocrystalline anisotropy (Kugrain). WATE is suppressed in the SUL by realizing wide distribution of magnetic flux below the edge of the return yoke, while spike noise is eliminated by ensuring the formation of a Néel wall instead of a Bloch wall in SUL domains. CoIr with the disordered hcp structure is selected as a negative Kugrain material, and c-plane-oriented CoIr films with various Ir contents are prepared for experimental evaluation. Among the films tested, the CoIr film with 22at.% Ir is found to provide the minimum Kugrain value of −6×106ergs∕cm3. Under a field applied parallel to the film plane, this film exhibits soft magnetic properties, attributable to the high crystallographic symmetry of the c-plane sheet texture. A PRM fabricated using the CoIr SUL is confirmed to display substantially lower spike noise and WATE compared to conventional structures.
The water rights situation: Increasing population and complexities of distribution of state's water supply comprise problem of major proportions (1957)

California Agriculture - All Issues
Evolution of competence and DNA uptake specificity in the Pasteurellaceae. (2006)

Competence and DNA uptake specificity are ancestral properties of the Pasteurellaceae, with divergent USSs and uptake specificity distinguishing only the two major subclades. The conservation of most competence genes over the approximately 350 million year history of the family suggests that lineages that lose competence may be evolutionary dead ends.
Über Die Gemeinsame Einwirkung Von Elementarem Schwefel Und Gasförmigem Ammoniak Auf Ketone II Die Gemeinsame Einwirkung Von Schwefel Und Ammoniak Auf Cyclohexanon (1957)

Abstract Die Einwirkung von elementarem Schwefel und gasförmigem Ammoniak auf Cyclohexanon führt bei Normaldruck und Raumtemperatur in fast quantitativ verlaufender Reaktion unter Abscheidung von Wasser zur Bildung von 2.2‐Pentamethylen‐4.5‐tetramethylen‐thiazolin‐Δ 3 (V) mit dem Schmp. 81.5—82°. Die Verbindung läßt sich in saurem Medium in Cyclohexanon, Ammoniak und 2‐Mercapto‐cyclohexanon spalten, das in seiner dimeren Form erhalten wird. Dabei bildet sich Bis‐2.3,5.6‐tetramethylen‐2.5‐endoxy‐1.4‐dithian. V kann auch durch Einwirkung von Cyclohexanon und gasförmigem Ammoniak auf 2‐Mercapto‐cyclohexanon hergestellt werden.
Collaboration management by smart voice messaging for physical and adaptive intelligent services (2013)

In light of the ongoing transformation from a traditional industrial society to a post-industrial society as a service society, it is unquestionably required to improve efficiency and quality of services. Especially, we focus on physical and adaptive intelligent services (PAI services) like nursing and caregiving where efficiency and quality are critical for an aging society. In these PAI services, several people have to collaborate to provide services, and a collaboration support system would be necessary. This paper proposes an information supervisory control model for a collaboration support system; furthermore, we have developed a smart voice messaging system based on this model. This model has a hypothesis that appropriate information sharing based on awareness of clients situation and task processes can be utilized and effective for improving efficiency and quality of services. We then formulate several hypotheses including the above one to be examined through field tests, virtual field tests, and simulation from the perspective of information supervisory control, and show some preliminary experimental results.
Streamlining workflow and automation to accelerate laboratory scale protein production (2017)

Protein production facilities are often required to produce diverse arrays of proteins for demanding methodologies including crystallography, NMR, ITC and other reagent intensive techniques. It is common for these teams to find themselves a bottleneck in the pipeline of ambitious projects. This pressure to deliver has resulted in the evolution of many novel methods to increase capacity and throughput at all stages in the pipeline for generation of recombinant proteins. This review aims to describe current and emerging options to accelerate the success of protein production in Escherichia coli. We emphasize technologies that have been evaluated and implemented in our laboratory, including innovative molecular biology and expression vectors, small-scale expression screening strategies and the automation of parallel and multidimensional chromatography.