gebhart/patrepeval huggingface dataset:
Growth of Vero E-6 cells on microcarriers in a cell bioreactor (1990)

Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated polystyrene (Kontes Life Sciences Products, Vineland, N.J.), Gelibead (Hazelton Research Products, Inc., Lenexa, Kans.), and Cytodex 3 (Pharmacia Fine Chemicals, Piscataway, N.J.), were used to compare the propagation of these anchorage-dependent cells. The number of viable cells recovered during three harvests over an 11-day incubation period was evaluated. The Vero E-6 cells grew equally well with both media. When the collagen-coated dextran microcarriers (Cytodex 3) were used, better cell attachment and higher-density growth were obtained and 5.5 x 10(8) to 7.0 x 10(8) cells were recovered from the system.
Mitomycin C and porfiromycin analogs with substituted ethylamines at position 7 (1983)

A series of 7-(2-substituted-ethyl)amino analogues of mitomycin C and porfiromycin was prepared and screened in standard antitumor systems. Certain of these analogues showed better activity than mitomycin C against P-388 leukemia, L-1210 leukemia, and/or B-16 melanocarcinoma in mice. Compounds also tested for their leukopenic effects in mice, the limiting toxicity of mitomycin C. Some of them were less leukopenic and some were more leukopenic than this clinical agent. No statistically significant correlations could be made between physicochemical properties and antitumor activities of the analogues.
Generation of Dominant Selectable Markers for Resistance to Pseudomonic Acid by Cloning and Mutagenesis of the <i>ileS</i> Gene from the Archaeon <i>Methanosarcina barkeri</i> Fusaro (2000)

Currently, only one selectable marker is available for genetic studies in the archaeal genus Methanosarcina. Here we report the generation of selectable markers that encode resistance to pseudomonic acid (PA(r)) in Methanosarcina species by mutagenesis of the isoleucyl-tRNA synthetase gene (ileS) from Methanosarcina barkeri Fusaro. The M. barkeri ileS gene was obtained by screening of a genomic library for hybridization to a PCR fragment. The complete 3,787-bp DNA sequence surrounding and including the ileS gene was determined. As expected, M. barkeri IleS is phylogenetically related to other archaeal IleS proteins. The ileS gene was cloned into a Methanosarcina-Escherichia coli shuttle vector and mutagenized with hydroxylamine. Nine independent PA(r) clones were isolated after transformation of Methanosarcina acetivorans C2A with the mutagenized plasmids. Seven of these clones carry multiple changes from the wild-type sequence. Most mutations that confer PA(r) were shown to alter amino acid residues near the KMSKS consensus sequence of class I aminoacyl-tRNA synthetases. One particular mutation (G594E) was present in all but one of the PA(r) clones. The MIC of pseudomonic acid for M. acetivorans transformed with a plasmid carrying this single mutation is 70 microgram/ml of medium (for the wild type, the MIC is 12 microgram/ml). The highest MICs (560 microgram/ml) were observed with two triple mutants, A440V/A482T/G594E and A440V/G593D/G594E. Plasmid shuttle vectors and insertion cassettes that encode PA(r) based on the mutant ileS alleles are described. Finally, the implications of the specific mutations we isolated with respect to binding of pseudomonic acid by IleS are discussed.
Selective Nucleation and Area-Selective OMCVD of Gold on Patterned Self-Assembled Organic Monolayers Studied by AFM and XPS: A Comparison of OMCVD and PVD (2000)

We demonstrate the area selective organometallic chemical vapor deposition (OMCVD) of ultrathin gold layers, using (trimethylphosphine)methylgold [(CH3)3P]AuCH3 as the gold source, onto self-assembled monolayers (SAMs) of ω-functionalized, long chain alkanethiols on gold and silver coated silicon and mica samples. The dependence on the reaction time of surface coverage with gold nano-clusters is analyzed by atomic force microscopy (AFM). X-ray photoelectron spectroscopy (XPS) shows that nucleation and growth occur only on thiol functionalized surfaces and not on methyl or hydroxyl functionalized SAMs. The selectivity of the growth is completely lost if gold is deposited by thermal evaporation of the pure metal, as shown in direct comparison with the OMCVD method employing mixed SAMs of different surface reactivity (–SH vs.–CH3) that were patterned by microcontact printing.
Scalar vs. parallel optimizations (2018)

In the realm of high performance and parallel computers, defining the order in which t o perform program optimizations and the interaction between optimizations is a difficult task. The compiler must perform some tradeoff between different optimizations, in particular trading traditional scalar optimizations against new parallel optimizations. Since the potential performance benefit of parallel optimizations can be a n order of magnitude higher, performing scalar pessimizations (inverting scalar optimizations) t o enable parallel optimizations can be the right overall decision. It is difficult t o treat parallel and scalar optimizations with a single framework, since they deal with different machine abstractions. We also show tha t no fixed ordering of optimizations will find the optimal transformed program; the ordering of optimizations must depend both on the target machine and on the program being compiled.
Functional expression of P-glycoprotein in Saccharomyces cerevisiae confers cellular resistance to the immunosuppressive and antifungal agent FK520. (1994)

We have recently reported that expression in yeast cells of P-glycoprotein (P-gp) encoded by the mouse multidrug resistance mdr3 gene (Mdr3) can complement a null ste6 mutation (M. Raymond, P. Gros, M. Whiteway, and D. Y. Thomas, Science 256:232-234, 1992). Here we show that Mdr3 behaves as a fully functional drug transporter in this heterologous expression system. Photolabelling experiments indicate that Mdr3 synthesized in yeast cells binds the drug analog [125I]iodoaryl azidoprazosin, this binding being competed for by vinblastine and tetraphenylphosphonium bromide, two known multidrug resistance drugs. Spheroplasts expressing wild-type Mdr3 (Ser-939) exhibit an ATP-dependent and verapamil-sensitive decreased accumulation of [3H]vinblastine as compared with spheroplasts expressing a mutant form of Mdr3 with impaired transport activity (Phe-939). Expression of Mdr3 in yeast cells can confer resistance to growth inhibition by the antifungal and immunosuppressive agent FK520, suggesting that this compound is a substrate for P-gp in yeast cells. Replacement of Ser-939 in Mdr3 by a series of amino acid substitutions is shown to modulate both the level of cellular resistance to FK520 and the mating efficiency of yeast mdr3 transformants. The effects of these mutations on the function of Mdr3 in yeast cells are similar to those observed in mammalian cells with respect to drug resistance and transport, indicating that transport of a-factor and FK520 in yeast cells is mechanistically similar to drug transport in mammalian cells. The ability of P-gp to confer cellular resistance to FK520 in yeast cells establishes a dominant phenotype that can be assayed for the positive selection of intragenic revertants of P-gp inactive mutants, an important tool for the structure-function analysis of mammalian P-gp in yeast cells.
Western blot identification of platelet proteins that bind normal serum immunoglobulins. Characteristics of a 95-Kd reactive protein (1990)

We have found that Western blots (WBs) of whole platelets exposed to normal autologous or homologous sera commonly have bands at 90 to 95 (95) Kd, and less often at 100 to 110, 80 to 85, 60 to 75, and 50 to 60 Kd when developed with antiglobulins. The percentages of normal sera producing a 95-Kd band with anti-immunoglobulin G (IgG), -IgA, and -IgM are 85, 50, and 30, respectively. Antiglobulin reagents alone also produce background bands on WBs that we have shown correspond to levels of platelet-associated Igs (PAIgs) or their derivatives. Titers of 95 Kd-reactive IgG in normal sera range from 10 to 1,280 (85% less than or equal to 50), and the reaction appears to be partially F(ab')2- mediated. The 95-Kd protein is internal and differs in many respects from surface glycoproteins IIIa, IV, and V of similar apparent molecular weight. In thrombocytopenic patients there was no correlation between severity of thrombocytopenia or PAIgG of platelet eluates and corresponding serum titers of 95 Kd-reactive IgG. Some WB reactions previously reported as evidence of autoimmunity may represent normal variations in reactions of Igs with internal platelet proteins. These reactions may be immunospecific or analogous to nonspecific, partially F(ab')2-dependent binding of Igs by certain bacterial proteins.
Asymmetric Organometallic-Catalyzed Reactions in Aqueous Media (2002)

This review provides an analysis of asymmetric organometallic-catalyzed reactions in aqueous media. The different methodologies for the preparation of chiral water-soluble ligands, and more particularly phosphorus derivatives, are summarized. The applications of these ligands in association with organometallic complexes in water only, as well as other conditions such as reaction in water in the presence of surfactants or in mixtures of water+organic solvents are described. Although asymmetric hydrogenation is one of the most studied catalyzed reactions in aqueous media, some preliminary results concerning asymmetric carbonylation, alkylation, oxidation, cyclopropanation, Diels–Alder reaction, and aldol reaction have appeared in the literature.
Minimizing Development Cost With Reliability Goal for Automotive Functional Safety During Design Phase (2017)

ISO 26262 is a functional safety standard specifically made for automotive systems, in which the automotive safety integrity level (ASIL) is the representation of the criticality level. Recently, most studies have used ASIL decomposition to reduce the development cost of automotive functions. However, these studies have not paid special attention to the problem that the reliability goal may not be satisfied when ASIL decomposition is performed. In this study, we solve the problem of minimizing the development cost of a distributed automotive function while satisfying its reliability goal during the design phase by presenting two heuristic algorithms, reliabilitycalculation of scheme (RCS) and minimizing development cost with reliability goal (MDCRG). We first use RCS to calculate the reliability value of each ASIL decomposition scheme; then, the MDCRG is used to select the scheme with the minimum development cost while satisfying the reliability goal. Real-life benchmark and simulated functions based on real parameter values are used in experiments, and results show the effectiveness of the proposed algorithms.
Functional Expression of a Heterologous Major Histocompatibility Complex Class I Gene in Transgenic Mice (1987)

The regulated expression of major histocompatibility complex class I antigens is essential for assuring proper cellular immune responses. To study H-2 class I gene regulation, we have transferred a foreign class I gene to inbred mice and have previously shown that the heterologous class I gene was expressed in a tissue-dependent manner. In this report, we demonstrate that these mice expressed the transgenic class I molecule on the cell surface without any alteration in the level of endogenous H-2 class I antigens. Skin grafts from transgenic mice were rapidly rejected by mice of the background strain, indicating that the transgenic antigen was expressed in an immunologically functional form. As with endogenous H-2 class I genes, the class I transgene was inducible by interferon treatment and suppressible by human adenovirus 12 transformation. Linkage analysis indicated that the transgene was not closely linked to endogenous class I loci, suggesting that trans-regulation of class I genes can occur for class I genes located outside the major histocompatibility complex.
Array-based Electromyographic Silent Speech Interface (2013)

An electromygraphic (EMG) Silent Speech Interface is a system which recognizes speech by capturing the electric potentials of the human articulatory muscles, thus enabling the user to communicate silently. This study is concerned with introducing an EMG recording system based on multi-channel electrode arrays. We first present our new system and introduce a method to deal with undertraining effects which emerge due to the high dimensionality of our EMG features. Second, we show that Independent Component Analysis improves the classification accuracy of the EMG array-based recognizer by up to 22.9% relative, which is a first example of an EMG signal processing method which is specifically enabled by our new array-based system. We evaluate our system on recordings of audible speech; achieving an optimal average word error rate of 10.9% with a training set of less than 10 minutes on a vocabulary of 108 words.
Mechanistic Aspects of Diels‐Alder Reactions: A Critical Survey (1980)

Abstract The question of concerted or consecutive bond formation arises in all types of cycloaddition reactions. Stereochemical investigations and studies of regiospecificity as well as intensive kinetic investigations with regard to substituent and solvent effects, and the dependence of the reaction on temperature and pressure permit in many cases mechanistic conclusions concerning the Diels‐Alder reaction. Efforts towards a theoretical interpretation, ab initio and semiempirical calculations, the application of frontier molecular orbital theory (FMO) as well as thermochemical measurements permit a description of the energy hypersurface of these [4+2]‐cycloadditions. An attempt is made here to draw a line of distinction between the mechanistic alternatives—one‐step reaction versus two‐step reaction—considering all experimental and theoretical criteria.
Synthetic alpha-thrombin receptor peptides activate G protein-coupled signaling pathways but are unable to induce mitogenesis. (1992)

alpha-Thrombin (thrombin) stimulates phospholipase C and modulates the activity of adenylate cyclase in a number of cell types via G protein-coupled receptors. It is also a potent growth factor, notably for a line of hamster fibroblasts (CCL39 cells). Recently, predicted amino acid sequences for human and hamster thrombin receptors have been reported that display a putative thrombin cleavage site in the N-terminal extracellular domain. Synthetic peptides corresponding to 14 residues carboxyl to the presumed thrombin cleavage site of the human receptor have been shown to activate platelets as well as the thrombin receptor expressed in Xenopus oocytes. In the present study we have examined the effects of synthetic peptides corresponding to the same region of the hamster receptor (S-42-L-55) and shorter peptides (2-7 residues) on signal transducing systems in CCL39 cells. Our results indicate that hamster receptor peptides of greater than or equal to 5 residues effectively stimulate phospholipase C in CCL39 cells via the thrombin receptor and induce rapid desensitization of the response. The same peptides also inhibit adenylate cyclase in a pertussis toxin-sensitive manner. Although the peptides are potent agonists of serotonin release in platelets, unlike thrombin, by themselves they are not mitogenic. However, they potentiate DNA synthesis in cooperation with growth factors possessing tyrosine kinase receptors. Hence, we conclude that the potent mitogenic action of thrombin cannot be accounted for solely by the activation of the cloned receptor. We postulate the existence of an additional receptor activated by thrombin, which is required for its full mitogenic potential.
Portable piezoelectric crystal detector for field monitoring of environmental pollutants (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPortable piezoelectric crystal detector for field monitoring of environmental pollutantsMat H. Ho, George G. Guilbault, and Bernd. ReitzCite this: Anal. Chem. 1983, 55, 11, 1830–1832Publication Date (Print):September 1, 1983Publication History Published online1 May 2002Published inissue 1 September 1983https://pubs.acs.org/doi/10.1021/ac00261a048https://doi.org/10.1021/ac00261a048research-articleACS PublicationsRequest reuse permissionsArticle Views58Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Load Indicating Washers (1989)

Kentucky Transportation Center (KTC) personnel monitored the installation of load indicating washers (LIWs) on the US 60 bridge at Catlettsburg, KY during construction. On completion of the bridge, KTC personnel performed torque measurements on a number of LIW installations. LIWs previously installed on the Thirteenth Street Bridge over the Ohio River at Ashland, KY were also inspected. The KTC construction inspections revealed no significant problems in installing LIWs. Workers were able to use them to determine proper bolt assembly installation. They also permitted inspectors to rapidly check for proper bolt tension. As with other bolt tensioning methods, LIWs had to be tested prior to installation. The follow-up field torque tests indicated that the LIWs provided consistent bolt tension values. The only potential service problem with LIWs, as discerned on the Thirteenth Street bridge, is related to corrosion. Uncoated LIWs must be hand painted to prevent rusting.
Selections from Lactantius: Divinae Institutiones, With Introduction, Commentary and Vocabulary. (2nd edition). By W. T. Radius. Grand Rapids, Michigan: Wm. B. Eerdmans, 1954. xviii, 139 pp. $2.50. (1959)

Selections from Lactantius: Divinae Institutiones, With Introduction, Commentary and Vocabulary. (2nd edition). By W. T. Radius. Grand Rapids, Michigan: Wm. B. Eerdmans, 1954. xviii, 139 pp. $2.50. - Volume 28 Issue 1
Qualification of a system technology for selective laser-based quasi-simultaneous soldering with an integrated pyrometric process control (2020)

Due to the tendency of miniaturization of electronic components, the increased use of thermally sensitive circuit boards e.g. LCDs, a large number of requirements arises for a selective laser beam soldering process. To enhance the possibilities of selective soldering, a system technology for quasi-simultaneous, pyrometric controlled laser soldering was qualified. The systems capability is to enable a controlled laser soldering process at different spot-sizes, varying electronic device layouts and different irradiation strategies. An inline emission measurement via pyrometer gains live-data from the soldering process, evaluates them and transfers them in a closed loop cycle to the laser power control. To enable a process control over a large scanning field, a chromatic corrected f-theta optic, which compensates the chromatic aberration between laser and pyrometer wavelength was used. Different scanning strategies and varying laser power profiles have been evaluated regarding their influence on the process quality.
Analysis of absorbing and leaky planar waveguides: a novel method (1989)

We present a simple and general approach for analysis of absorbing and leaky waveguides. The real and imaginary parts of the propagation constant beta of a planar optical waveguide are obtained by evaluating a function, defined through the eigenvalue equation, in real beta. The applicability of the method is demonstrated for simple structures. To apply the method for arbitrarily graded inhomogeneous or multilayered structures we use a simple matrix approach to obtain the eigenvalue equation. The method is straightforward, accurate, and requires no iteration in the complex beta plane.
γ‐Secretase component presenilin is important for microglia β‐amyloid clearance (2010)

We suggest for the first time, a dual role for γ-secretase in Alzheimer's disease. One role is the cleavage of the amyloid precursor protein for pathologic β-amyloid production and the other is to regulate microglia activity that is important for clearing neurotoxic β-amyloid deposits. Further studies of γ-secretase-mediated cellular pathways in microglia may provide useful insights into the development of Alzheimer's disease and other neurodegenerative diseases, providing future avenues for therapeutic intervention.
Intelligent agent based auction by economic generation scheduling for microgrid operation (2010)

This paper presents the implementation of distributed controls in a microgrid operation. The approach utilizes the advantages of using Multi Agent Systems technology for controlled operation of a microgrid. The auctioneer aims to optimize the operation of the microgrid by optimizing the production of local distributed generators. An artificial immune system based algorithm is applied on a typical study case network assuming realistic market prices for power and distributed generators bids reflecting realistic operational costs. Simulation results clearly indicate that the agent based control framework is effective in coordinating the various distributed energy resources economically.
Design, development, and fabrication of a portable spectrometer-type monitor Technical summary report, 29 Jun. 1964 - 31 Jan. 1966 (1966)

Portable spectrometer for monitoring oxygen and hydrogen as free gas or as dissociation products in inert gas shield of gas tungsten arc welding process
Structural Stability of Iodide Perovskite: A Combined Cluster Expansion Method and First-Principles Study (2017)

To aid the development of Pb-free perovskite solar cells, the stability of the iodide perovskite structure ABI3 has been investigated by first-principles calculations, Bader charge analysis, and the cluster expansion method. At the A sites, methylammonium (MA, CH3NH3+), formamidinium (FA, CH(NH2)2+), and Cs+ were modeled, while at the B sites, one or two elements from Pb, Sn, Ge, In, Ga, Bi, and Sr were examined. For the partially substituted system A(B,B′)I3, we found that the stability strongly depends on the identity of the A-site cation. For example, Cs(B,B′)I3 structures are stabilized by a mixture of divalent cations, such as Pb, Sn, and Ge, at the B site. Concerning the stabilization mechanisms, Coulomb energy gain seems to be the origin of the structural stability in A = Cs structures. From our results, Cs(B,B′)I3, where the B site is occupied by divalent cations, are possible candidates for high stability, lead-free solar cell materials.
Experience with bovine arteriografts for haemodialysis. (1979)

Twenty-five bovine arteriografts were placed in 18 patients for maintenance of haemodialysis. Type of vascular access, complications and their management and long-term results are discussed.