gebhart/patrepeval huggingface dataset:
NMR of <sup>13</sup>C Enriched Amino Acids and Peptides (1982)

The combination of isotopic enrichment and nuclear magnetic resonance spectroscopy provides an extremely powerful biochemical/biophysical probe. The magnetic properties (e.g. chemical shift, relaxation behavior) and the metabolic fates of individual nuclei can be studied in systems as complex as whole cells. Thus, the advantages of sensitivity gains, spectral simplification and spin labeling are the primary motivations for isotopic enrichment. In addition to these more obvious aspects of isotopic labeling, there are unique problems of interpretation and sources of information which require careful analysis and which represent important considerations in experimental design. Some of these aspects of the problem can be approached most directly by considering the spectral characteristics of relatively simple systems such as labeled amino acids and peptides. Such studies thus lay the groundwork for more sophisticated applications of isotopic labeling. The primary objective of the present article is to outline some of the characteristics of labeled systems, with
Gas-phase Microsequencing of Peptides and Proteins with a Fluorescent Edman-type Reagent, Fluorescein Isothiocyanate (1994)

A fluorescent Edman-type reagent, fluorescein isothiocyanate (FITC), was adapted to a gas-phase sequencer fitted with a miniature reaction chamber. Fluorescein thiohydantion amino acids were identified by on-line gradient HPLC with fluorescence monitoring. The optimized protocol for the coupling reaction using FITC and phenylisothiocyanate, and the degree of washing to remove the excess reagents were established. Myoglobin (5 pmol) and lysozyme (5 pmol) were analyzed by the sequencer to obtain a 35-51% initial yield (I.Y.) and 82-88% repetitive yield (R.Y.) for the former, and 51-66% I.Y. and 91-92% R.Y. for the latter. These figures permitted 20-25 amino acid residues to be identified from the N-terminus of 5 pmol samples applied to the sequencer.
Data-Parallel Line Relaxation Method for the Navier-Stokes Equations (1998)

The Gauss‐Seidel line relaxation method is modie ed for the simulation of viscous e ows on massively parallel computers. The resulting data-parallel line relaxation method is shown to have good convergence properties for a seriesoftestcases.Thenewmethodrequiressignie cantlymorememorythanthepreviouslydevelopeddata-parallel relaxation methods, but it reaches a steady-state solution in much less time for all cases tested to date. In addition, the data-parallel line relaxation method shows good convergence properties even on the high-cell-aspect-ratio grids required to simulate high-Reynolds-number e ows. The new method is implemented using message passing on the Cray T3E, and the parallel performance of the method on this machine is discussed. The data-parallel line relaxation method combines the fast convergence of the Gauss ‐Seidel line relaxation method with a high parallel efe ciency and thus shows promise for large-scale simulation of viscous e ows.
Partial Agonistic Activity of Naloxone on the Opioid Receptors Expressed from Complementary Deoxyribonucleic Acids in Chinese Hamster Ovary Cells (1998)

Naloxone is a widely used opioid antagonist.To analyze the cellular responses induced by naloxone in the absence of opioid agonists, Chinese hamster ovary (CHO) cells, which do not endogenously express the opioid receptors, have been permanently transfected with the cloned complementary DNAs to produce the [micro sign]-, delta-, and kappa-opioid receptors. Naloxone dose-dependently reduced forskolin-stimulated cyclic adenosine monophosphate (cAMP) formation in the cells expressing the [micro sign]- and kappa-opioid receptors, although the effect was less than that of opioid agonists [D-Ala2, N-Me-Phe4, Gly-ol5]enkephalin and U50,488, respectively. The naloxone-induced cAMP reduction was abolished by pretreatment of the cells with pertussis toxin, which suggests that pertussis toxin-sensitive G proteins (Gi and/or Go) are involved in the response. Cellular guanosine triphosphatase activity was significantly increased by naloxone in the cells expressing the [micro sign]- and kappa-opioid receptors, which suggests that the application of naloxone to these receptors induces activation of the G proteins. We conclude that naloxone possesses partial agonistic activity on the [micro sign]- and kappa-opioid receptors expressed from complementary DNAs in CHO cells. Implications: In this study, we examined whether naloxone has agonistic activity on the opioid receptors by using cultured cells transfected with delta-, [micro sign]-, and kappa-opioid receptor complementary DNAs. Our data indicate that naloxone is a partial agonist on the [micro sign]- and kappa-opioid receptors. (Anesth Analg 1998;87:450-5)
Targeting of Porcine Pancreatic Phospholipase A2 to Human Platelets: Introduction of an RGD Sequence by Genetic Engineering (1995)

The possibility to induce specific disruption of activated platelets by binding of porcine pancreatic phospholipase A2 (PLA2) was tested by constructing a set of PLA2-mutants containing an Arg-Gly-Asp (RGD) sequence. One mutant was made with RGD as part of a surface-exposed loop (RGDloop). Four mutants were made with RGD as part of a C-terminal extension: one with RGD directly coupled to the C-terminus (RGDc) and three mutants (CRSx) with x= 22, 42 and 82 hydrophylic non-charged amino acids between RGD and the enzyme. All mutants retained 20-80% activity of native PLA2 and showed little binding to resting platelets. The binding of the native enzyme and RGDloop was not increased following stimulation. In contrast, the mutants RGDc and CRSx showed stimulation-dependent binding to the platelet receptor GPIIb/IIIa, since GRGDS-peptide and a monoclonal antibody against the complex interfered with binding. In alpha-thrombin-stimulated platelets, CRS42 and CRS82 induced about 5% hydrolysis of [3H]-arachidonic acid-labeled phospholipids. Stimulation with a combination of alpha-thrombin and collagen (known to expose phosphatidylserine) increased hydrolysis to 11%. Despite the membrane disruption, the cells did not leak lactate dehydrogenase. We conclude that PLA2 can be targeted to activated platelets by introducing RGD in a C-terminal extension with a minimum distance (42 amino acids) between RGD and the enzyme. However, more hydrolytic activity is required to eliminate activated platelets among a suspension of resting platelets and other blood cells.
Universidad-empresa: un estudio histórico-político de la conformación del CETRI Litoral (2010)

This paper analyzes, from a historical-political point of view, the shaping of university-industry\nrelations in terms of the emergence of a political realm made possible by the emergence and\ndevelopment of a new epistemic regime: the politicized science and technology. The case of\nstudy analyzed is the constitution of the Center for Transfer of Research Results (CETRI Litoral)\nof the Universidad Nacional del Litoral, which is the result of the cooperation with the Center for\nTechnology Transfer (CTT) of the Universidad Politécnica de Valencia. This work uses a\nconceptual framework to grasp the formation of university-industry ties and the emergence of a\npolitical realm which constitute new forms of legitimacy for the university to emerge and are\nconsolidated from the processes of self-understanding built by agents operating in the university\ngovernance. We also analyze how it enhances the emergence of a new ethos in the research\ncommunity that promotes university-industry relations. This work builds its explanatory\npondering specific processes occurring within the Universidad Nacional del Litoral, that plot with\nglobal processes of transformation of the mode of social existence of science and technology.
Improved Gene Targeting through Cell Cycle Synchronization (2015)

Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell cycle arrest, we obtained improved gene targeting rates in Yarrowia lipolytica, Arxula adeninivorans, Saccharomyces cerevisiae, Kluyveromyces lactis and Pichia pastoris demonstrating the broad applicability of the method. Hydroxyurea treatment enriches for S-phase cells that are active in homologous recombination and enables previously unattainable genomic modifications.
Noise Suppression Methods for Robust Speech Processing (1978)

Abstract : Robust speech processing in practical operating environments requires effective environmental and processor noise suppression. This report describes the technical findings and accomplishments to develop real time, compressed speech analysis-synthesis algorithms whose performance is invariant under signal contamination. Fulfillment of this requirement is necessary to insure reliable secure compressed speech transmission within realistic military command and control environments. Overall contributions resulting from this research program include the understanding of how environmental noise degrades narrow band, coded speech, development of appropriate real time noise suppression algorithms, and development of speech parameter identification methods that consider signal contamination as a fundamental element in the estimation process. This report describes results in the areas of noise suppression using the spectral subtraction algorithm, dual input adaptive noise cancellation using the LMS algorithm, pole-zero parameter estimation, nonparametric-rank order statistics with applications to Robust Speech Activity detection, spectral analysis and synthesis using the constant-Q transform, and pitch and rate changes to speech using the constant-Q transform.
139. Heterocyclic fluorine compounds. Part IV. Monofluoroindazoles (1960)

I. K. Barben and H. Suschitzky, J. Chem. Soc., 1960, 672 DOI: 10.1039/JR9600000672
Proteomic Characterization of Postmortem Amyloid Plaques Isolated by Laser Capture Microdissection (2004)

The presence of amyloid plaques in the brain is one of the pathological hallmarks of Alzheimer's disease (AD). We report here a comprehensive proteomic analysis of senile plaques from postmortem AD brain tissues. Senile plaques labeled with thioflavin-S were procured by laser capture microdissection, and their protein components were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified a total of 488 proteins co-isolated with the plaques, and we found multiple phosphorylation sites on the neurofilament intermediate chain, implicating the complexity and diversity of cellular processes involved in the plaque formation. More significantly, we identified 26 proteins enriched in the plaques of two AD cases by quantitative comparison with surrounding non-plaque tissues. The localization of several proteins in the plaques was further confirmed by the approach of immunohistochemistry. In addition to previously identified plaque constituents, we discovered novel association of dynein heavy chain with the plaques in human postmortem brain and in a double transgenic AD mouse model, suggesting that neuronal transport may play a role in neuritic degeneration. Overall, our results revealed for the first time the sub-proteome of amyloid plaques that is important for further studies on disease biomarker identification and molecular mechanisms of AD pathogenesis.
Evaluation of platinum-DNA adduct levels relative to known prognostic variables in a cohort of ovarian cancer patients. (1990)

Single-agent chemotherapy with cisplatin or carboplatin can induce remissions in approximately 30% of previously treated patients with advanced stage ovarian cancer. Previous studies have shown that the extent of platinum-DNA adduct formation measured in WBC DNA of ovarian cancer patients treated with cisplatin or carboplatin is directly associated with disease response (Reed et al., Proc. Natl. Acad. Sci. USA, 84: 5024-5028, 1987). It has been unclear whether adduct level in WBC DNA is independent of known prognostic variables in this disease, or whether adduct level parallels a known prognostic variable that can be more easily monitored. In a cohort of 24 ovarian cancer patients treated with single-agent cisplatin or carboplatin, we retrospectively assessed the relationship between disease response, platinum-DNA adducts in WBC DNA, and each of eight prognostic variables by both univariate analysis and multivariate analysis. The prognostic variables evaluated included: response to previous treatment, Karnofsky status, total platinum dose prior to current therapy, stage of disease, age, bulk of disease at initiation of therapy, histological type, and histological grade. By univariate analysis, adduct level was strongly associated with disease response (two-sided P = 0.0058), with the next strongest associations with disease response being held by Karnofsky status (P = 0.125), stage of disease (P = 0.189), response to previous treatment (P = 0.352), total previous platinum dose (P = 0.358), and age (P = 0.374). No significant associations were found between adduct level and histological type or histological grade. Further, when patients were stratified by the number of cycles studied (one cycle, two cycles, or three cycles), higher levels of adduct were consistently seen in those patients responding to therapy. We conclude that, in this small cohort of refractory ovarian cancer patients treated with single-agent cisplatin or carboplatin, adduct level in WBC DNA appears to be more closely related to disease response than other previously identified prognostic variables.
The Influence of a Horseshoe Vortex on Local Convective Heat Transfer (1990)

The objective of this research program has been to determine experimentally the extent to which horseshoe vortices modify turbulent convective heat transfer along a flat plate downstream of an appendage. The importance of appendage shape on the heat transfer behavior was evaluated by taking Stanton-number measurements downstream of both a cylindrical body and a streamlined body. The results indicate that a region of augmented heat transfer occurs near the centerline downstream of both obstacles, with Stanton numbers 10 to 50 percent over the undisturbed values. The streamlined cylinder produces the strongest modifications in heat transfer.
Facial nerve stimulation in normal pigs and healthy human volunteers: transitional development of a medical device for the emergency treatment of ischemic stroke (2018)

The minimal effective stimulation parameters defined by increased CBF, as identified in the pig study, translated into safe, tolerable, and effective stimulation of healthy volunteers. These results support the future development and evaluation of non-invasive facial nerve stimulation for the emergency treatment of ischemic stroke. Trial Registration retrospectively registered with clinicaltrials.gov NRV_P1_01_15 on June 6, 2017.
Binding and regulation of HIF‐1α by a subunit of the proteasome complex, PSMA7 (2001)

The hypoxia-inducible factor-1alpha (HIF-1alpha) is an important transcription factor for cellular responses to oxygen tension. It is rapidly degraded under normoxic conditions by the ubiquitin-dependent proteasome pathway. Here we report a critical role of the 20S proteasome subunit PSMA7 in HIF-1alpha regulation. PSMA7 was found to interact specifically with two subdomains of HIF-1alpha. PSMA7 inhibited the transactivation function of HIF-1alpha under both normoxic and hypoxia-mimicking conditions. In addition, we show that the PSMA7-mediated regulation of HIF-1alpha activity is associated with the proteasome pathway.
Thermal bonding of polypropylene films and fibers (2008)

Abstract The thermal bonding behavior of different grades of polypropylene was studied in this research. Initial bonding studies were done with polypropylene films of different grades of polymer with varying morphology, and the studies were extended to polypropylene fibers. Polypropylene fibers manufactured with different cross‐sections, deniers, polymer melt‐flow rates, and different processing conditions were bonded under various conditions. The effect of film structure, properties, and bonding conditions on the bonding efficiency was studied by comparing the tensile strength of bonded films. Thermal bonding was carried for a range of temperatures covering poor, optimum, and over bonding. Changes taking place to the polymer in bond point and the original surface were analyzed using the SEM. Higher bond strength was observed in the vicinity of melting temperature and the strength reduced with a further increase in bonding temperature. The frequent failure point was observed at the bond edge crossover points where film undergoes maximum thickness transition. Lower pressure and shorter time were found to be appropriate for bonding. Films with lower orientation formed better bonds. The optimum bond strength and the optimum bonding temperature observed were different for different polymers. Fiber bond strength results were similar to the results observed in the case of films with respect to the bonding temperatures studied. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Immunomodulating effect of human placenta‐eluted gamma globulins in rheumatoid arthritis (1982)

Patients who had active and severe rheumatoid arthritis (RA) were treated with human placenta-eluted gamma globulins (PEGG) composed of purified IgG eluted from trophoblastic cells. A control group of RA patients received retroplacental gamma globulins with no clinical effects. Seven of eleven treatments with PEGG showed good (4 patients) or very good (3 patients) clinical results. All patients had immunologic modifications demonstrated by a significant increase in active E-rosette-forming cells and mitogen responsiveness. The precise mechanism of immunomodulating effect of PEGG should be further investigated.
Procedure abstraction recovery from binary code (2002)

Binary translation (the automatic translation of executable programs from one machine to another) requires analyses and transformations that could be used in a wide variety of tools intended to reverse engineer binary codes. Our approach to binary translation, which is designed to allow both source and target machines to be changed at low cost, is based on a combination of machine descriptions, binary interface descriptions and machine-independent analyses. This paper deals with the recovery of high-level procedure calls from binary code (i.e. the recovery of parameters and return locations) in a machine-independent way. The use of a specification language called PAL (Procedure Abstraction Language) is described, as well as the machine-independent recovery analysis based on PAL. The work described in this paper has been integrated into UQBT (University of Queensland Binary Translator), which is a resourceable binary translation framework. Translations across binaries for SPARC/sup TM/, Pentium and Java/sup TM/ virtual machine architectures have been achieved.
The Beat Goes On, 1965–76 (2010)

The world of Senator Goldwater and the conservatives who championed his candidacy were handed humility on November 3, 1964. The thoughts of millions of Americans were now on trying to carry out the promises started by President John F. Kennedy and reinforced by President Lyndon Johnson during the 1964 campaign. What would citizen Goldwater now do? He would have to wait until a Senate seat was open, which would be in 1968, to run again.KeywordsPresidential ElectionVice PresidentRepublican PartyMilitary DraftWatergate ScandalThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
X-ray Phase Imaging Using Lau Effect (2011)

X-ray phase imaging based on the Lau effect is demonstrated with an incoherent laboratory X-ray source. Its optical configuration resembles the inverse geometry of the X-ray Talbot–Lau interferometer, which employs a large-area amplitude grating. However, the proposed approach avoids the use and hence fabrication difficulty of such a grating, which is advantageous when constructing an X-ray phase imaging apparatus. With a Mo-target source, differential phase images of polymer spheres were successfully obtained and a design concept for downsizing the system is also described.
DIETARY n-6 AND n-3 FATTY ACID BALANCE AND CARDIOVASCULAR HEALTH (2004)

▪ Abstract Epidemiological and clinical studies have established that the n-6 fatty acid, linoleic acid (LA), and the n-3 fatty acids, linolenic acid (LNA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) collectively protect against coronary heart disease (CHD). LA is the major dietary fatty acid regulating low-density lipoprotein (LDL)-C metabolism by downregulating LDL-C production and enhancing its clearance. Further, the available mass of LA is a critical factor determining the hyperlipemic effects of other dietary fat components, such as saturated and trans fatty acids, as well as cholesterol. By contrast, n-3 fatty acids, especially EPA and DHA, are potent antiarryhthmic agents. EPA and DHA also improve vascular endothelial function and help lower blood pressure, platelet sensitivity, and the serum triglyceride level. The distinct functions of these two families make the balance between dietary n-6 and n-3 fatty acids an important consideration influencing cardiovascular health. Based on published literature describing practical dietary intakes, we suggest that consumption of ∼6% en LA, 0.75% en LNA, and 0.25% en EPA + DHA represents adequate and achievable intakes for most healthy adults. This corresponds to an n-6/n-3 ratio of ∼6:1. However, the absolute mass of essential fatty acids consumed, rather than their n-6/n-3 ratio, should be the first consideration when contemplating lifelong dietary habits affecting cardiovascular benefit from their intake.
Influence of Fibrin Network Conformation and Fibrin Fiber Diameter on Fibrinolysis Speed (2000)

Abnormal fibrin architecture is thought to be a determinant factor of hypofibrinolysis. However, because of the lack of structural knowledge of the process of fibrin digestion, relationships between fibrin architecture and hypofibrinolysis remain controversial. To elucidate further structural and dynamic changes occurring during fibrinolysis, cross-linked plasma fibrin was labeled with colloidal gold particles, and fibrinolysis was followed by confocal microscopy. Morphological changes were characterized at fibrin network and fiber levels. The observation of a progressive disaggregation of the fibrin fibers emphasizes that fibrinolysis proceeds by transverse cutting rather than by progressive cleavage uniformly around the fiber. Plasma fibrin clots with a tight fibrin conformation made of thin fibers were dissolved at a slower rate than those with a loose fibrin conformation made of thicker (coarse) fibers, although the overall fibrin content remained constant. Unexpectedly, thin fibers were cleaved at a faster rate than thick ones. A dynamic study of FITC-recombinant tissue plasminogen activator distribution within the fibrin matrix during the course of fibrinolysis showed that the binding front was broader in coarse fibrin clots and moved more rapidly than that of fine plasma fibrin clots. These dynamic and structural approaches to fibrin digestion at the network and the fiber levels reveal aspects of the physical process of clot lysis. Furthermore, these results provide a clear explanation for the hypofibrinolysis related to a defective fibrin architecture as described in venous thromboembolism and in premature coronary artery disease.