gebhart/patrepeval huggingface dataset:
ELECTRICAL, MAGNETIC, AND OPTICAL PROPERTIES OF π-CONJUGATED POLYMER NANOTUBES AND NANOWIRES (2004)

Nanotubes and nanowires of π-conjugated polypyrrole (PPy) and poly (3,4-ethylenedioxythiophene) were synthesized using Al 2 O 3 nanoporous template through electrochemical polymerization method. From the SEM and TEM photographs, the formation of conducting polymer nanotube (CPNT) and nanowire (CPNW) was confirmed. From FT-IR and UV/Vis absorbance spectra, we observed the effect of doping and de-doping through HF or NaOH dissolving of Al 2 O 3 template. DC conductivity and I–V characteristics as a function of temperature and gate bias were measured for the CPNTs and CPNWs prepared with various synthetic conditions. Magnetic properties were measured through EPR experiments. Based on the results, we compare the intrinsic properties between bulk and nanoscale π-conjugated polymers.
Hericenones and erinacines: stimulators of nerve growth factor (NGF) biosynthesis in<i>Hericium erinaceus</i> (2010)

This review surveys the chemical and biological literature dealing with the isolation, structural elucidation and bioactivity of hericenones and erinacines from the fruiting body and mycelium of Hericium erinaceus, concentrating on work that has appeared in the literature up to December 2009.
Characterization of the binding of purified human C1q to heart mitochondrial membranes. (1981)

The binding of purified, radioiodinated human C1q to baboon heart mitochondrial membranes was investigated. The interaction of C1q with heart mitochondrial membranes was shown to be readily saturable, specific for C1q, and reversible upon addition of unlabeled C1q or increasing salt concentrations. Scatchard plots of the binding data were biphasic and yielded association constants on the order of 1 X 10(10) and 1 X 10(9) M-1 and binding capacities of approximately 0.16 and 0.24 nmol of C1q/mg of mitochondrial protein. The binding of C1q to isolated cardiac-derived mitochondrial membranes is implicated in the antibody-independent activation of the classical complement pathway by cellular membranes.
Passive treatment of coal mine drainage. Information circular/1994 (1993)

Passive methods of treating mine water use chemical and biological processes that decrease metal concentrations and neutralize acidity. Compared with conventional chemical treatment, passive methods generally require more land area, but use less costly reagents and require less operational attention and maintenance. Currenlty, three types of passive technologies exist: aerobic wetlands, organic substrate wetlands, and anoxic limestone drains. Aerobic wetlands promote mixed oxidation and hydrolysis reactions, and are most effective when the raw mine water is net alkaline. Organic substrate wetlands promote anaerobic bacterial activity that results in the precipitation of metal sulfides and the generation of bicarbonate alkalinity. Anoxic limestone drains generate bicarbonate alkalinity and can be useful for the pretreatment of mine water before it flows into a wetland.
Chemiluminescence fiber optic probe for hydrogen peroxide based on the luminol reaction (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemiluminescence fiber optic probe for hydrogen peroxide based on the luminol reactionThompson M. Freeman and W. Rudolf. SeitzCite this: Anal. Chem. 1978, 50, 9, 1242–1246Publication Date (Print):August 1, 1978Publication History Published online1 May 2002Published inissue 1 August 1978https://pubs.acs.org/doi/10.1021/ac50031a012https://doi.org/10.1021/ac50031a012research-articleACS PublicationsRequest reuse permissionsArticle Views540Altmetric-Citations110LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Structural and Functional Analysis of the C-Terminus of Gαq in Complex with the Human Thromboxane A<sub>2</sub>Receptor Provides Evidence of Constitutive Activity (2010)

The human thromboxane A2 (TXA2) receptor (TP) is known to mediate platelet aggregation and vasoconstriction. The receptor predominantly interacts with the Gq protein, thereby activating phospholipase C and increasing the intracellular calcium level. In this study, we synthesized a 15-residue peptide corresponding to the C-terminal domain of the Gq protein α subunit (Gαq-Ct peptide) and characterized its interaction with recombinant TP purified from a baculovirus expression system in the presence and absence of an agonist using fluorescence and NMR spectroscopic studies. With fluorescence binding assays, we demonstrated that the Gαq-Ct peptide was bound to TP, in the absence of the agonist, with a Kd value of approximately 17 μM. Interestingly, upon addition of the agonist, U46619, the Gαq-Ct peptide’s binding affinity for this activated TP was reduced, thereby increasing the Kd value to approximately 240 μM. NMR experiments demonstrated that the TP-bound Gαq-Ct peptide shows a different affinity and conformation, in the absence and presence of the agonist, U46619. This suggested there is the possibility of ligand-free constitutive TP signaling through Gα binding. Thus, an HEK293 cell line that stably expresses human TP and lacks the ability to produce TXA2 was created by gene transfer and G418 selection. In comparison with the control cells, the stable cell line showed significant Gα-mediated ligand-free calcium signaling. The study indicates a promising new outlook for the examination of prostanoid receptor−G-protein interactions in greater detail using integrated NMR spectroscopy, the purified receptor, and the stable cell line.
In vitro complement-dependent binding and in vivo kinetics of pneumococcal polysaccharide TI-2 antigens in the rat spleen marginal zone and follicle (1996)

For a better understanding of the spleen-dependent induction of the humoral immune response against thymus-independent type 2 antigens, we have studied the in vitro and in vivo localization of different capsular pneumococcal polysaccharides (PPSs) in the rat spleen. In this study, we found that in vitro binding of PPS types 3, 4, 6B, 9N/V, 14, and 23F was dependent on complement (probably a C3 fragment) and that the localization was predominantly restricted to the marginal-zone B lymphocytes and the follicular dendritic cells. In vivo, we observed with increase of time a shift of localized antigens. Shortly after injection, all PPS types localized in the marginal-zone B lymphocytes, then localized in the outer follicular mantle, and finally were found to be diffuse in the complete follicle and follicle corona. PPS types 3 and 9N/V and later also PPS type 23F localized additionally in red pulp macrophages. In particular, the localization in the marginal zone is important since the low flow in this area in combination with strongly CD21+ B cells, which are activated early, gives a maximum opportunity for the induction of a primary humoral immune response with subsequent differentiation into plasma cells or migration to the germinal center. In addition, the localization of PPSs at follicular dendritic cells should be considered important in the induction of an ongoing immune response not restricted to the spleen.
Genetic Requirements for Potassium Ion-Dependent Colony Spreading in<i>Bacillus subtilis</i> (2005)

Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but independent of flagella, as verified with a mutant with a hag gene replacement; this latter finding highlights the importance of sliding motility in colony spreading. Exploring the K(+) requirement, directed mutagenesis of the higher-affinity K(+) transporter KtrAB, but not the lower-affinity transporter KtrCD, was found to inhibit surface colonization unless sufficient KCl was added. To identify other genes involved in K(+)-dependent colony spreading, transposon insertion mutants in wild-type strain 3610 were screened. Disruption of genes for pyrimidine (pyrB) or purine (purD, purF, purH, purL, purM) biosynthetic pathways abolished the K(+)-dependent spreading phase. Consistent with a requirement for functional nucleic acid biosynthesis, disruption of purine synthesis with the folic acid antagonist sulfamethoxazole also inhibited spreading. Other transposon insertions disrupted acetoin biosynthesis (the alsS gene), acidifying the growth medium, glutamine synthetase (the glnA gene), and two surfactin biosynthetic genes (srfAA, srfAB). This work identified four classes of surface colonization mutants with defective (i) potassium transport, (ii) surfactin formation, (iii) growth rate or yield, or (iv) pH control. Overall, the ability of B. subtilis to colonize surfaces by spreading is highly dependent on balanced nucleotide biosynthesis and nutrient assimilation, which require sufficient K(+) ions, as well as growth conditions that promote sliding motility.
A 9 pW/Hz adjustable clock generator with 3-decade tuning range for dynamic power management in subthreshold SCL systems (2010)

A widely-tunable and power-scalable clock generator for ultra-low power (ULP) applications is presented. Benefitting from a novel self-adjustable loop frequency response, the proposed phase-locked loop based clock generator exhibits a tuning range of three decades. Implemented in 0.13 μm CMOS, the circuit occupies 0.06 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , while its power dissipation is 9 pW/Hz, proportional to the output clock frequency with 350 nW stand-by power. The circuit remains stable with scalable dynamics for frequency steps (upward and downward) as large as a factor of ×1024. The presented clock generator has been designed to be compatible with subthreshold source-coupled logic (STSCL) topology that can be used for ultra-low power applications such as in bio-medical systems.
Evaluation of platelet glycoprotein Ib by fluorescence flow cytometry (1985)

Platelet glycoprotein Ib (GpIb), a receptor for von Willebrand's factor (vWF), was studied by way of fluorescence flow cytometry. Using a sandwich staining technique, GpIb was identified by a monoclonal antibody (6D1) directed against an epitope close to the vWF binding site. Platelets from normal individuals were symmetrically distributed with respect to GpIb content. Treatment of washed platelets with plasmin resulted in progressive loss of GpIb as measured by fluorescence flow cytometry and by loss of agglutination response when combined with ristocetin in the presence of vWF. In mixing experiments with GpIb-deficient and normal platelets, it was possible to detect a subpopulation of deficient cells comprising 2% of the total population. Streptokinase treatment of platelet-rich plasma caused loss of the agglutination response to ristocetin and the emergence of a population of GpIb-deficient platelets. Fluorescence flow cytometry appears to be an important new technique by which to study platelet surface receptors.
A Portable Slurry Packing Apparatus for High Performance Liquid Chromatography (1979)

Abstract A portable apparatus for slurry packing high performance microparticulate liquid chromatographic columns is described. The packer offers lab to lab portability and ease of operations. Columns up to 50 cm in length can be accommodated with the described configuration. Material costs per column are $40 – $70, offering a substantial saving over commercially prepared columns, in situations where labor costs are minimal. Efficiencies of columns packed with this apparatus have been equivalent to or better than those obtained with commercially available columns. Columns are packed using a staged pressurization/depressurization method. This method also leads to longer column life. Evaluation of relative column efficiency is also discussed.
SOY-NEW HEALTH BENEFITS ASSOCIATED WITH AN ANCIENT FOOD (2000)

Soy has been a dietary staple in many Asian diets for centuries. Scientists recently have become increasingly interested in components of soy and its possible contribution to disease prevention and overall health. This article discusses the potential impact of soy protein and various biologically active components associated with soy protein on cardiovascular disease, menopausal symptoms, and cancer. It also addresses protein quality and the potential for incorporating soy foods into the American diet.
Response to immune checkpoint inhibition and survival in <i>BRCA-</i>associated recurrent ovarian cancer. (2019)

2615 Background: Alterations in the DNA mismatch repair pathway increase susceptibility to immune checkpoint inhibition (ICI). Tumors with BRCA-related DNA repair defects may have increased antigenicity, which could drive response to ICI. Responses to ICI in ovarian cancer (OC) have been modest. We seek to evaluate the association of BRCA mutations with response to ICI and survival in recurrent OC. Methods: A single-center, retrospective review identified 103 women with recurrent OC and known BRCA mutation status (90 germline and 33 somatic testing) who received ICI between 1/2013-7/2018 (98 on study). Clinical characteristics and duration of ICI (Long > = 24 vs. Short < 24 weeks) were compared by BRCA status. Kaplan Meier survival analysis was used to calculate progression-free (PFS) and overall survival (OS) from start of ICI, and CoxPH models/logrank test were used to assess survival differences by BRCA status. Results: Deleterious germline (g) or somatic (s) BRCA 1/2 mutations were present in 29 (28%) women (12 g BRCA1, 9 g BRCA2, 3 s BRCA1, 5 s BRCA2, 1 g BRCA1/s BRCA1, 3 g BRCA2/s BRCA2, and 1 g BRCA2/s BRCA1). Patients (pts) with BRCA mutations had more lines of treatment prior to ICI (median 5 vs. 4, p = 0.03) and a longer time from diagnosis to ICI (median 54 vs. 38.5 months (mo), p = 0.01), but there were no significant differences in other variables including histology (86% high grade serous), stage at diagnosis (96% Stage III/IV), and platinum status (83% resistant), p > 0.05. Four pts (15%) with BRCA mutations had long duration of ICI as compared with 20 pts (27%) in those without mutations, p = 0.20. Median PFS was 2.2 mo (95% CI 1.7-2.7) in those with BRCA mutations and 3.4 mo (95% CI 2.1-4.0) in those without mutations, HR 1.22 (95% CI 0.78-1.91, p = 0.38). At a median follow-up of 23.3 mo, median OS was 21.3 mo (95% CI 13.7-31.8) in those with BRCA mutations and 19.8 mo (95% CI 13.8-25.3) in those without. This was not significantly different, HR 1.00 (95% CI 0.54-1.87, p = 0.99), after adjustment for prior lines and time from diagnosis to ICI. Conclusions: In our study of heavily pretreated OC pts receiving ICI, BRCA 1/2 mutations were not associated with improved response or survival. These findings should be validated in larger studies.
Effects of laser vs ultrasound on bone healing after distraction osteogenesis: A histomorphometric analysis (2014)

This study concluded that bone healing is accelerated with the application of laser irradiation. The greatest effects were observed with combined ultrasound and laser treatment.
Effect of Dehydration on the Formation of Levofloxacin Pseudopolymorphs. (1995)

Differential scanning calorimetry (DSC) curves of levofloxacin hemihydrate measured under various conditions showed different thermograms. These phenomena were attributed to be the dehydration. Dehydration caused a multiple-phase transition. Dehydration at a higher temperature (above 70°C) gave a sharp endothermic peak on the DSC curve due to the melting of the γ form, and at a lower temperature (below 50°C) gave a sharp endothermic peak due to the melting of the α form.In contrast, the thermal behavior of levofloxacin monohydrate was not affected by dehydration. The difference in the thermal behavior between the hemihydrate and the monohydrate might be attributed to a difference in the interaction between levofloxacin and crystal water. Observations by thermomicroscopy, the changes in powder X-ray diffraction patterns during heating, and single X-ray analysis all supported the above interpretation.
Engineering microbial biofuel tolerance and export using efflux pumps (2011)

Biofuels can be produced by microbes, but biofuel toxicity is a major obstacle to efficient production. Here, the authors identify efflux pumps that can effectively export biofuels, improving cell viability and increasing biofuel yields.
Optimal Features Subset Selection and Classification for Iris Recognition (2008)

The selection of the optimal features subset and the classification have become an important issue in the field of iris recognition. We propose a feature selection scheme based on the multiobjectives genetic algorithm (MOGA) to improve the recognition accuracy and asymmetrical support vector machine for the classification of iris patterns. We also suggest a segmentation scheme based on the collarette area localization. The deterministic feature sequence is extracted from the iris images using the 1D log-Gabor wavelet technique, and the extracted feature sequence is used to train the support vector machine (SVM). The MOGA is applied to optimize the features sequence and to increase the overall performance based on the matching accuracy of the SVM. The parameters of SVM are optimized to improve the overall generalization performance, and the traditional SVM is modified to an asymmetrical SVM to treat the false accept and false reject cases differently and to handle the unbalanced data of a specific class with respect to the other classes. Our experimental results indicate that the performance of SVM as a classifier is better than the performance of the classifiers based on the feedforward neural network, the k-nearest neighbor, and the Hamming and the Mahalanobis distances. The proposed technique is computationally effective with recognition rates of 99.81% and 96.43% on CASIA and ICE datasets, respectively.
Neutrophil microvesicles resolve gout by inhibiting C5a-mediated priming of the inflammasome (2015)

PMN-Ecto-mediated control of inflammasome-driven inflammation is a compelling concept of autoregulation initiated early on during PMN activation in gout.
Contribution of Multiparameter Flow Cytometry Immunophenotyping to the Diagnostic Screening and Classification of Pediatric Cancer (2013)

Pediatric cancer is a relatively rare and heterogeneous group of hematological and non-hematological malignancies which require multiple procedures for its diagnostic screening and classification. Until now, flow cytometry (FC) has not been systematically applied to the diagnostic work-up of such malignancies, particularly for solid tumors. Here we evaluated a FC panel of markers for the diagnostic screening of pediatric cancer and further classification of pediatric solid tumors. The proposed strategy aims at the differential diagnosis between tumoral vs. reactive samples, and hematological vs. non-hematological malignancies, and the subclassification of solid tumors. In total, 52 samples from 40 patients suspicious of containing tumor cells were analyzed by FC in parallel to conventional diagnostic procedures. The overall concordance rate between both approaches was of 96% (50/52 diagnostic samples), with 100% agreement for all reactive/inflammatory and non-infiltrated samples as well as for those corresponding to solid tumors (n = 35), with only two false negative cases diagnosed with Hodgkin lymphoma and anaplastic lymphoma, respectively. Moreover, clear discrimination between samples infiltrated by hematopoietic vs. non-hematopoietic tumor cells was systematically achieved. Distinct subtypes of solid tumors showed different protein expression profiles, allowing for the differential diagnosis of neuroblastoma (CD56(hi)/GD2(+)/CD81(hi)), primitive neuroectodermal tumors (CD271(hi)/CD99(+)), Wilms tumors (>1 cell population), rhabdomyosarcoma (nuMYOD1(+)/numyogenin(+)), carcinomas (CD45(-)/EpCAM(+)), germ cell tumors (CD56(+)/CD45(-)/NG2(+)/CD10(+)) and eventually also hemangiopericytomas (CD45(-)/CD34(+)). In summary, our results show that multiparameter FC provides fast and useful complementary data to routine histopathology for the diagnostic screening and classification of pediatric cancer.
Efficient mining of association rules in text databases (1999)

In this paper, we propose two new algorithms for mining association rules between words in text databases. The characteristics of text databases are quite different from those of retail transaction databases, and existing mining algorithms cannot handle text databases efficiently because of the large number of itemsets (i.e., words) that need to be counted. Two well-known mining algorithms, Apriori algorithm and Direct Hashing and Pruning (DHP) algorithm, are evaluated in the context of mining text databases, and are compared with the new proposed algorithms named Multipass-Apriori (M-Apriori) and Multipass-DHP (M-DHP). It has been shown that the proposed algorithms have better performance for large text databases.
T-lymphocyte subpopulations in relation to immunosuppression in measles and varicella (1983)

Patients with measles or varicella were studied during the acute phase (first week) of illness and, after recovery, by lymphocyte stimulation tests and determination of T-lymphocyte subpopulations, using the monoclonal antibodies Leu 2a and Leu 3a directed at the suppressor/cytotoxic and the helper T-cell subsets, respectively. Low proliferative responses to phytohemagglutinin were found during the acute phase of both diseases. The response to purified protein derivate of tuberculin was also low in all measles patients tested and in some of the varicella patients. In both infections increased spontaneous DNA synthesis was demonstrated. In the acute phase of measles there was T lymphocytopenia but no change of the ratio between T lymphocytes of helper and suppressor/cytotoxic cell phenotypes. In the acute phase of varicella the percentage and the absolute number of Leu 2-positive (suppressor/cytotoxic) T cells were increased. Measurement of the size of the lymphocytes indicated activation of this subset. Cryopreserved blood mononuclear cells from the acute phase of varicella could suppress the phytohemagglutinin response of autologous convalescent-phase cells. This was not seen when cells from measles patients were tested. The suppression of the lymphocyte stimulation response in varicella is probably in part caused by activation of suppressor cells, whereas the suppression of the stimulation response in measles seems to be due mainly to other mechanisms.