gebhart/patrepeval huggingface dataset:
Amine Functionalization of Collagen Matrices with Multifunctional Polyethylene Glycol Systems (2010)

A method to functionalize collagen-based biomaterials with free amine groups was established in an attempt to improve their potential for tethering of bioactive molecules. Collagen sponges were incorporated with amine-terminated multifunctional polyethylene glycol (PEG) derivatives after N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide and N-hydroxysuccinimide (EDC/NHS) cross-linking. The extent of the incorporation of different amounts and different numbers of active moieties of amine-terminated PEG systems into the collagen scaffolds was evaluated using ninhydrin assay, Fourier transform infrared spectrophotometry (FTIR), collagenase degradation assay, denaturation temperature measurements, and in vitro cell studies. A 3% 8-arm amine-terminated PEG was found to be the minimum required effective concentration to functionalize EDC/NHS stabilized collagen scaffolds. EDC/NHS stabilized scaffolds treated with 3% 8-arm amine-terminated PEG exhibited significantly improved denaturation temperature and resistance to collagenase degradation over non-cross-linked scaffolds (p < 0.002). Biological evaluation using 3T3 cells demonstrated that the produced scaffolds facilitated maintenance of the cells' morphology, metabolic activity, and ability to proliferate in vitro. Overall, our results indicate that amine-terminated PEG systems can be used as means to enhance the functionality of collagenous structures.
Spinal Cord Injury in the Pediatric Population: A Systematic Review of the Literature (2011)

Spinal Cord Injury (SCI) in the pediatric population is relatively rare but carries significant psychological and physiological consequences. An interdisciplinary group of experts composed of medical and surgical specialists treating patients with SCI formulated the following questions: 1) What is the epidemiology of pediatric spinal cord injury and fractures?; 2) Are there unique features of pediatric SCI which distinguish the pediatric SCI population from adult SCI?; 3) Is there evidence to support the use of neuroprotective approaches, including hypothermia and steroids, in the treatment of pediatric SCI? A systematic review of the literature using multiple databases was undertaken to evaluate these three specific questions. A search strategy composed of specific search terms (Spinal Cord Injury, Paraplegia, Quadriplegia, tetraplegia, lapbelt injuries, seatbelt injuries, cervical spine injuries and Pediatrics) returned over 220 abstracts that were evaluated and by two observers. Relevant abstracts were then evaluated and papers were graded using the Downs and Black method. A table of evidence was then presented to a panel of experts using a modified Delphi approach and the following recommendation was then formulated using a consensus approach: Pediatric patients with traumatic SCI have different mechanisms of injury and have a better neurological recovery potential when compared to adults. Patients with SCI before their adolescent growth spurt have a high likelihood of developing scoliosis. Because of these differences, traumatic SCI should be highly suspected in the presence of abnormal neck or neurological exam, a high-risk mechanism of injury or a distracting injury even in the absence of radiological anomaly.
In Situ Assembly and Screening of Enzyme Inhibitors with Surface‐Tension Microarrays (2009)

Hundreds of reactions were conducted in parallel in droplets maintained on a glass slide through differential surface tension in a new approach to submicroliter-scale synthesis. This “surface-tension microarray” was applied to the in situ assembly of thousands of derivatives of phenylboronic acid and their profiling against the NS3/4A protease of the hepatitis C virus. Several potent inhibitors of the enzyme were identified.
A Differential Transformer with Temperature- and Excitation-Independent Output (1972)

This paper describes a new concept for compensating for sensitivity changes of a differential transformer due to variations in temperature and excitation conditions. The idea is based on utilization of the individual outputs from the secondary coils, not only as a differential output but also as a reference output. The differential transformer usually has two secondary coils connected in opposition to obtain a differential output. If the voltages induced in the individual secondary coils e1 and e2 are individually taken out and if the operation (e1 - e2)/(e1 + e2), is carried out, then the result becomes independent of temperature and excitation conditions. The experiments, which were made to confirm the effectiveness of this method, gave quite impressive and hopeful results: superior temperature, excitation independence and linearity.
Sequence analysis, tissue distribution, and expression of rat cathepsin S. (1992)

Cysteine proteases are involved in many diverse cellular processes ranging from processing of precursor proteins to intracellular degradation. In an effort to identify novel cysteine proteases, we used the polymerase chain reaction and primers directed against the catalytic sites of previously cloned cysteine proteases. From rat brain mRNA, a 600-base pair band was amplified; cloning and partial sequence analysis of this band resulted in the identification of cathepsins B and L and five novel sequences. The novel cDNAs contained a number of residues conserved in lysosomal cysteine proteases, including the active site residue His159 (papain numbering). In addition, the amino acid homology between the novel sequences and either cathepsins B, L, or H, ranged from 63 to 32%. The insert with highest homology was used to screen a rat brain cDNA library; a 1334-base pair cDNA was isolated and the nucleotide sequence determined. This sequence encodes an open reading frame of 330 amino acids which is 82% homologous to human cathepsin S, suggesting that this sequence represents rat cathepsin S. Northern blot analysis for rat cathepsin S revealed tissue-specific expression distinct from the distribution of cathepsin B and L. The regulation of expression of rat cathepsin S mRNA in response to thyroid-stimulating hormone was studied in a rat thyroid cell line FRTL-5. The level of cathepsin S mRNA was substantially increased in response to thyroid-stimulating hormone, whereas cathepsin B and cathepsin L mRNA levels were not altered by this treatment. A portion of cDNA encoding the predicted mature protein of rat cathepsin S was expressed as a glutathione S-transferase-fusion protein. The affinity-purified protein exhibited proteolytic activity with properties similar to bovine cathepsin S. Taken together, these results imply highly specific functions for cathepsin S.
Characterization of a proton-driven carrier for sialic acid in the lysosomal membrane (1989)

Highly purified lysosomal membrane vesicles, obtained from rat liver lysosomes, were used to study characteristics of NeuAc transport across the lysosomal membrane. Uptake of [14C]NeuAc was found to be strongly influenced by a pH gradient across the membrane. When a proton gradient (pHin greater than pHout) was generated by impermeable buffers, NeuAc uptake above equilibrium level (overshoot) was observed. The influence of membrane diffusion potentials was ruled out by experiments where K+ and valinomycin were present. The overshoot appeared to be specifically produced by protons, since gradients of other cations (Na+ and K+) did not give stimulation. Proton-driven uptake was saturable (Kt = 0.24 mM) and mediated by a single system, as shown by linearity of the Scatchard plot. Stimulation of transport was also obtained by preincubation of vesicles with MgATP and the effect was blocked by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, but not by the protonophore carbonyl cyanide p-trifluoromethoxyphenyl hydrazone. Monocarboxylic sugars like glycuronic acids were competitive inhibitors of sialic acid transport. Transstimulation of [14C] NeuAc uptake was observed when vesicles were preloaded either with unlabeled NeuAc or with glucuronic acid. The data demonstrate that lysosomal membrane vesicles from rat liver are a suitable system for kinetic studies of solute transport events. The presence of a proton-driven carrier in the lysosomal membrane specific for sialic acid and other acidic sugars, including glucuronic acid, is shown. The possible physiological significance of these findings for the human lysosomal carrier and the patients with a sialic acid transport defect is discussed.
Driving cycle development for electric vehicle application using principal component analysis and k-means cluster: with the case of Shenyang, China (2017)

Using a typical driving cycle to implement and evaluate the established control strategy is quite essential in the investigation of electric vehicles (EVs) power management issue. How to build a representative driving cycle remains a challenge due to the complex urban driving conditions. In this paper, the principal component analysis (PCA) and k-means cluster are employed to develop the driving cycle with case of Shenyang, China. First of all, a large amount of road conditions test data are collected, which are made up of a series of data including driving time and the instantaneous velocity. On top of that, the PCA is applied to extract the main components of overall road information and the K-means cluster is used to select representative kinematic fragments. Several most representative fragments are chosen to form the driving cycle. At last, the proposed driving cycle is simulated and verified. The result shows that the proposed driving cycle can well match to overall road information.
The Use of Clay-Polymer Nanocomposites in Wastewater Pretreatment (2012)

Some agricultural effluents are unsuitable for discharge into standard sewage-treatment plants: their pretreatment is necessary to avoid clogging of the filtering devices by colloidal matter. The colloidal stability of the effluents is mainly due to mutual repulsive forces that keep charged particles in suspension. Pretreatment processes are based on two separate stages: (a) neutralization of the charges ("coagulation") and (b) bridging between several small particles to form larger aggregates that sink, leaving clarified effluent ("flocculation"). The consequent destabilization of the colloidal suspension lowers total suspended solids (TSSs), turbidity, and other environmental quality parameters, making the treatments that follow more efficient. Clay-based materials have been widely used for effluent pretreatment and pollutant removal. This study presents the use of nanocomposites, comprised of an anchoring particle and a polymer, as "coagoflocculants" for the efficient and rapid reduction of TSS and turbidity in wastewater with a high organic load. The use of such particles combines the advantages of coagulant and flocculant by neutralizing the charge of the suspended particles while bridging between them and anchoring them to a denser particle (the clay mineral), enhancing their precipitation. Very rapid and efficient pretreatment is achieved in one single treatment step.
Effects of Y Dopant on Lattice Distortion and Electrical Properties of In<sub>3</sub>SbTe<sub>2</sub> Phase‐Change Material (2017)

Using a computational high‐throughput screening method, 29 doping elements have been investigated for improving the thermal and electrical characteristics of In 3 SbTe 2 (IST) phase‐change material. Among the 29 dopants, it is found that Y offers largest distortion in the lattice structure of IST with negative doping formation energy while Y substitutes the In site. The atomic lattice images clearly show that the In site is substituted by Y and the distortion angles of the Y‐doped IST (Y‐IST) are well matched with the calculated results of density functional theory (DFT). Set/reset speed of the Y‐IST phase‐change memory is faster than IST and Ge 2 Sb 2 Te 5 (GST) devices, which is strongly related with the fast and stable phase transition due to the larger lattice distortion. The power consumption of the Y‐IST device is also less than a fourth of that of the GST device.
Copper-Catalyzed Asymmetric Conjugate Addition of Alkenyl- and Alkylalanes to α,β-Unsaturated Lactams (2013)

Alkenyl and alkyl groups have been successfully introduced to six-membered α,β-unsaturated lactams via a copper-catalyzed asymmetric 1,4-addition of the corresponding alanes. Moderate to good yields and good to excellent enantioselectivities are achieved by using a combination of the very cheap copper(II) naphthenate and a readily available phosphine amine ligand. The creation of an all-carbon quaternary stereogenic center, via Michael addition to a trisubstituted conjugated lactam, is also disclosed for the first time.
CpG methylation as a mechanism for the regulation of E2F activity (2000)

Regulation of gene expression in mammals through methylation of cytosine residues at CpG dinucleotides is involved in the development and progression of tumors. Because many genes that are involved in the control of cell proliferation are regulated by members of the E2F family of transcription factors and because some E2F DNA-binding sites are methylated in vivo, we have investigated whether CpG methylation can regulate E2F functions. We show here that methylation of E2F elements derived from the dihydrofolate reductase, E2F1, and cdc2 promoters prevents the binding of all E2F family members tested (E2F1 through E2F5). In contrast, methylation of the E2F elements derived from the c-myc and c-myb promoters minimally affects the binding of E2F2, E2F3, E2F4, and E2F5 but significantly inhibits the binding of E2F1. Consistent with these studies, E2F3, but not E2F1, activates transcription through methylated E2F sites derived from the c-myb and c-myc genes whereas both E2F1 and E2F3 fail to transactivate a reporter gene that is under the control of a methylated dihydrofolate reductase E2F site. Together, these data illustrate a means through which E2F activity can be controlled.
Clock synchronization through handshake signalling (2004)

We present a method for synchronizing pausible clocks in GALS (Globally Asynchronous, Locally Synchronous) systems. In contrast to most conventional GALS schemes the method is not based on including in each ring oscillator a synchronizing element (such as for instance an arbiter) which on one side can pause the clock and on the other side offers a handshake interface. Instead, we propose a scheme in which each synchronous module has both an incoming and an outgoing clock signal, which have been obtained by opening the module's ring oscillator. Since these clock signals also behave as handshake signals, handshake circuits can be used to synchronize the clocks. We demonstrate the technique in the context of processors and memories. All the designs have been simulated and showed functionally correct.
PERFORMANCE OF A LARGE SCALE, NON-EQUILLIBRIUM MHD GENERATOR WITH RARE GASES. PART II. (1970)

Noble gas mixture large shock tunnel driven linear MHD generator, examining electron density, operating characteristics and electrical properties
Frontal Screens on Head-Mounted Displays to Increase Awareness of the HMD Users' State in Mixed Presence Collaboration (2019)

In the everyday context, e.g., a household, HMD users remain a part of the social life for Non-HMD users being co-located with them. Due to the social context situations arise that demand interaction between the HMD and the Non-HMD user. We focus on the challenge that the Non-HMD user is not able to interpret the HMD user's state -- e.g., attentiveness; the need for assistance --, as the HMD covers the wearer's face. We propose a front facing display attached to the HMD that supports collaboration by showing the state. We explore the impact of abstract and realistic visualizations for such displays on collaborative performance and social presence in a within-subject user study (N=25). We present to the Non-HMD user (1) a blank screen (baseline), (2) textual representation of the user's state and (3) a representation that looks like the HMD is see-through. The results show positive effects for textual representation on collaborative performance and a positive effect of realistic representation on social presence. We conclude that when developing HMDs we need to take into account the social needs of everyday life to reduce the risk of social separation in a household context.
Automatic acquisition of high-fidelity facial performances using monocular videos (2014)

This paper presents a facial performance capture system that automatically captures high-fidelity facial performances using uncontrolled monocular videos ( e.g ., Internet videos). We start the process by detecting and tracking important facial features such as the nose tip and mouth corners across the entire sequence and then use the detected facial features along with multilinear facial models to reconstruct 3D head poses and large-scale facial deformation of the subject at each frame. We utilize per-pixel shading cues to add fine-scale surface details such as emerging or disappearing wrinkles and folds into large-scale facial deformation. At a final step, we iterate our reconstruction procedure on large-scale facial geometry and fine-scale facial details to further improve the accuracy of facial reconstruction. We have tested our system on monocular videos downloaded from the Internet, demonstrating its accuracy and robustness under a variety of uncontrolled lighting conditions and overcoming significant shape differences across individuals. We show our system advances the state of the art in facial performance capture by comparing against alternative methods.
Nitric oxide causes ADP-ribosylation and inhibition of glyceraldehyde-3-phosphate dehydrogenase. (1992)

Nitric oxide and nitric oxide-generating agents like 3-morpholinosydnonimine (SIN-1) stimulate the mono-ADP-ribosylation of a cytosolic, 39-kDa protein in various tissues. This protein was purified from human platelet cytosol by conventional and fast protein liquid chromatography techniques. N-terminal sequence analysis identified the isolated protein as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Nitric oxide stimulates the auto-ADP-ribosylation of GAPDH in a time and concentration-dependent manner with maximal effects after about 60 min. Associated with ADP-ribosylation is a loss of enzymatic activity. NAD(+)-free enzyme is not inhibited by SIN-1, indicating the absolute requirement of NAD+ as the substrate of the ADP-ribosylation reaction. Inhibition of the glycolytic enzyme GAPDH may be relevant as a cytotoxic effect of NO complementary to its inhibitory actions on iron-sulfur enzymes like aconitase and electron transport proteins of the respiratory chain.
Real-time optical holographic tracking of multiple objects (1989)

A coherent optical correlation technique for real-time simultaneous tracking of several different objects making independent movements is described, and experimental results are presented. An evaluation of this system compared with digital computing systems is made. The real-time processing capability is obtained through the use of a liquid crystal television spatial light modulator and a dichromated gelatin multifocus hololens. A coded reference beam is utilized in the separation of the output correlation plane associated with each input target so that independent tracking can be achieved.
Patient-size-dependent radiation dose optimisation technique for abdominal CT examinations (2011)

Since patient doses from computed tomography (CT) are relatively high, risk-benefit analysis requires dose to patients and image quality be optimised. The aim of this study was to develop a patient-dependent optimisation technique that uses patient diameter to select a combination of CT scanning parameters that minimise dose delivered to patients undergoing abdominal CT examinations. The study was performed using cylindrical phantoms of diameters ranging from 16 to 40 cm in order to establish the relationship between image degradation, CT scanning techniques, patient dose and patient size from two CT scanners. These relationships were established by scanning the phantoms using standard scanning technique followed by selected combinations of scanning parameters. The image noises through phantom images were determined using region of interest software available in both scanners. The energy depositions to the X-ray detector through phantoms were determined from measurements of CT dose index in air corrected for attenuation of the phantom materials. The results demonstrate that exposure settings (milliampere seconds) could be reduced by up to 82 % for smaller phantom relative to standard milliampere seconds, while detector signal could be reduced by up to 93 % for smaller phantom relative to energy depositions required when scanned using standard scanning protocols. It was further revealed that the use of the object-specific scanning parameters on studies performed with phantom of different diameters could reduce the incident radiation to small size object by up to 86 % to obtain the same image quality required for standard adult object. In view of the earlier mentioned fact, substantial dose saving from small-sized adults and children patients undergoing abdomen CT examinations could be achieved through optimal adjustment of CT scanning technique based on the patient transverse diameter.
Channel Quality Indication Reporting Schemes for UTRAN Long Term Evolution Downlink (2008)

In this paper we evaluate the performance of channel quality indicator (CQI) reporting schemes in 3GPP UTRAN long term evolution (LTE) downlink (DL). In LTE, time and frequency dependent CQI is needed for DL packet scheduling (PS) and fast link adaptation (LA). Studies have indicated that frequency domain PS (FD-PS) and LA are essential techniques in improving the LTE performance, giving e.g. both cell throughput and coverage gain of around 40 % over a distributed multiplexing scheme. However, there is a tradeoff with signaling overhead related to the CQI feedback and overall LA and PS performance, which is rather overlooked in the literature. We analyze four different CQI reporting schemes with respect to system spectral efficiency and conclude that the best-M average and threshold based CQI reporting schemes seem to be the most promising in terms of the compromise between system performance and signaling overhead.
An improved HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products. (2003)

An improved HPLC qualitative and quantitative method of six triterpenes (asiaticoside, madecassoside, asiatic acid, madecassic acid, terminolic acid, and asiaticoside-B) in Centella asiatica (raw plant material and preparations) is described in this paper. After 50 minutes the six active triterpenes were separated and detected in the methanolic extract at a limit of 0.01 microg/ml. The method uses a Phenomenex Aqua 5mu C18 (200 A) column as the stationary phase, a gradient mobile phase of water (0.1% TFA), acetonitrile (0.1% TFA), and methyl tert-butyl ether (0.1% TFA), and UV detection at 206 nm. The correlation coefficients for the calibration curves and the recovery rates ranged from 0.995 to 0.999 and from 98.39% to 100.02%, respectively. The qualitative and quantitative results are discussed.
The Discovery of Polyacetylene Film: The Dawning of an Era of Conducting Polymers (Nobel Lecture) (2001)

This lecture is not directly related to our discovery and development of conducting polymers to which the Nobel Prize in Chemistry 2000 was awarded. However, I would like to present my previous work that I had carried out just before we reached the discovery of chemical doping. I hope that this will be of use and deepen your understandings by learning what had happened before and how we reached the idea of chemical doping.