gebhart/patrepeval huggingface dataset:
WT1 mutation in 470 adult patients with acute myeloid leukemia: stability during disease evolution and implication of its incorporation into a survival scoring system (2010)

The impact of WT1 mutations in acute myeloid leukemia (AML) is not completely settled. We aimed to determine the clinical implication of WT1 mutation in 470 de novo non-M3 AML patients and its stability during the clinical course. WT1 mutations were identified in 6.8% of total patients and 8.3% of younger patients with normal karyotype (CN-AML). The WT1 mutation was closely associated with younger age (P < .001), French-American-British M6 subtype (P = .006), and t(7;11)(p15;p15) (P = .003). Multivariate analysis demonstrated that the WT1 mutation was an independent poor prognostic factor for overall survival and relapse-free survival among total patients and the CN-AML group. A scoring system incorporating WT1 mutation, NPM1/FLT3-ITD, CEBPA mutations, and age into survival analysis proved to be very useful to stratify CN-AML patients into different prognostic groups (P < .001). Sequential analyses were performed on 133 patients. WT1 mutations disappeared at complete remission in all WT1-mutated patients studied. At relapse, 3 of the 16 WT1-mutated patients who had paired samples lost the mutation and 2 acquired additional mutations, whereas 3 of 110 WT1-wild patients acquired novel mutations. In conclusion, WT1 mutations are correlated with poor prognosis in AML patients. The mutation status may be changed in some patients during AML progression.
Optimized PCR using Vent polymerase. (1994)

PCR has become an essential tool of the molecular biologist, and substantial literature exists characterizing the performance of Taq polymerase in this reaction. (~-4) Use of a thermostable polymerase that, unlike Taq, possesses 3'---> 5' exonuclease activity is desirable in situations where higher fidelity and/ or blunt-end products are required. (s--8) Vent polymerase is one such enzyme, but information on optimal use of this enzyme in PCR is limited. Ling et al. identified conditions that optimize the fidelity of PCR using Vent polymerase. (s) We commonly need high-fidelity blunt PCR products for use in DNA constructions. Our initial experience with Vent polymerase convinced us that, within the previously defined conditions that optimize fidelity, careful optimization for PCR product quantity in addition to quality was essential. Therefore, we systematically examined a number of potentially important PCR conditions in a stepwise manner. This analysis led to a highly optimized procedure described below.
Static and dynamic semantics processing (1991)

Article Free Access Share on Static and dynamic semantics processing Authors: Charles Consel Department of Computer Science, Yale University, P.O. Box 2158, New Haven, CT Department of Computer Science, Yale University, P.O. Box 2158, New Haven, CTView Profile , Olivier Danvy Department of Computing and Information Sciences, Kansas State University, Manhattan, KS Department of Computing and Information Sciences, Kansas State University, Manhattan, KSView Profile Authors Info & Claims POPL '91: Proceedings of the 18th ACM SIGPLAN-SIGACT symposium on Principles of programming languagesJanuary 1991 Pages 14–24https://doi.org/10.1145/99583.99588Online:03 January 1991Publication History 35citation515DownloadsMetricsTotal Citations35Total Downloads515Last 12 Months14Last 6 weeks4 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Multi-Keyword Searchable and Data Verifiable Attribute-Based Encryption Scheme for Cloud Storage (2019)

In a data sharing system, it is a basic requirement for a user, who has an appropriate privilege to perform keyword retrieval for encrypted documents stored in the cloud. Although traditional searchable encryption technology can provide data protection and retrieval characteristic, there are some main issues should also be considered. First, most existing attribute-based searchable encryption schemes only support single-keyword search, which may return abundant irrelevant search results, resulting in a waste of computational and broadband resources. Second, the user often needs to seek some data related to some particular keywords but his attributes may be altered frequently. Third, the cloud server is not completely loyal which sometimes returns a fraction of erroneous search results. Focus on these issues, a practical multi-keyword searchable encryption scheme is proposed for data integrity verification and attribute revocation by combining the ciphertext policy attribute-based encryption (CP-ABE) and auditing ideas. The scheme on one hand supports multi-keyword search which avoids the cloud server yield ample irrelevant documents by narrowing the search scope, and the other hand can implement effectively attribute revocation by entrusting ciphertext updates to the powerful cloud server, thereby preventing access by illegal users. Furthermore, third-party audits use verification algorithms to ensure the correctness of search results and reduce the amount of computing by end users. The most critically, the scheme proved to be resistant to selective plaintext attacks and selective keyword attacks under the general group model. The extensive experimental results demonstrate that the scheme is more expressive, efficient, and feasible in the practical applications.
Discovery of a proteinaceous cellular receptor for a norovirus (2016)

Noroviruses (NoVs) are a leading cause of gastroenteritis globally, yet the host factors required for NoV infection are poorly understood. We identified host molecules that are essential for murine NoV (MNoV)-induced cell death, including CD300lf as a proteinaceous receptor. We found that CD300lf is essential for MNoV binding and replication in cell lines and primary cells. Additionally, Cd300lf(-/-) mice are resistant to MNoV infection. Expression of CD300lf in human cells breaks the species barrier that would otherwise restrict MNoV replication. The crystal structure of the CD300lf ectodomain reveals a potential ligand-binding cleft composed of residues that are critical for MNoV infection. Therefore, the presence of a proteinaceous receptor is the primary determinant of MNoV species tropism, whereas other components of cellular machinery required for NoV replication are conserved between humans and mice.
Technique for determining the fibre distribution in the z-direction using confocal microscopy and image analysis (1997)

Confocal microscopy provides a nondestructive method for sectioning a sheet into a large number of discrete layers. Fibres in each layer can be identified using image analysis, by devising a technique that separates them from air and artefacts. In this paper, the image collection process using a confocal microscope is first briefly described and then the methods used to find fibre area in an image are discussed. A dynamic thresholding method is suggested to measure fibre area accurately. As an example, the fibre distribution from the top surface to 50 micrometres deep within a 60 g/m 2 handsheet is examined. The result shows that the top 20 micrometres of the sheet has an open structure and the fibre percentage varies between 50 and 70% at a depth of 20-50 μm.
Grafica, Storia, Italia: estendere il campo / Graphic Design, History, Italy: Expanding the Field (2013)

Testo introduttivo del numero 24 della rivista dedicato allo stato della storiografia sul graphic design italiano.
Combined Nurr1 and Foxa2 roles in the therapy of Parkinson's disease (2015)

Use of the physiological mechanisms promoting midbrain DA (mDA) neuron survival seems an appropriate option for developing treatments for Parkinson's disease (PD). mDA neurons are specifically marked by expression of the transcription factors Nurr1 and Foxa2. We show herein that Nurr1 and Foxa2 interact to protect mDA neurons against various toxic insults, but their expression is lost during aging and degenerative processes. In addition to their proposed cell-autonomous actions in mDA neurons, forced expression of these factors in neighboring glia synergistically protects degenerating mDA neurons in a paracrine mode. As a consequence of these bimodal actions, adeno-associated virus (AAV)-mediated gene delivery of Nurr1 and Foxa2 in a PD mouse model markedly protected mDA neurons and motor behaviors associated with nigrostriatal DA neurotransmission. The effects of the combined gene delivery were dramatic, highly reproducible, and sustained for at least 1 year, suggesting that expression of these factors is a promising approach in PD therapy.
DBP occurrence survey (1997)

Evidence gathered by the American Water System suggests USEPA should revisit the proposed Stage I DBP regulations, especially TOC removal requirements and related conditions for prechlorination. Results of a disinfection by‐product (DBP) survey and two years of distribution system DBP monitoring are presented for more than 100 treatment plants of the American Water System (AWS), the largest investor‐owned water utility in the United States. Monitoring of total organic carbon (TOC) indicated that 83 percent of the AWS surface water treatment plants that exceed a raw water TOC concentration of 2 mg/L will not receive predisinfection credit for the use of chlorine. With existing treatment practices, approximately 20 and 66 percent of the AWS water treatment plants studied will exceed the proposed Stage I and Stage II trihalomethane (THM) maximum contaminant levels (MCLs) of 80 and 40 μg/L, respectively. Similarly, approximately 16 and 52 percent of the AWS systems studied will exceed the proposed Stage I and II haloacetic acids (HAA5) MCLs of 60 and 30 μg/L, respectively. Based on these estimates, it is believed that Stage II requirements may significantly affect future water treatment practices and costs.
L'ITINERAIRE D'UN HISTORIEN DE L'AMERIQUE LATINE. ENTRETIEN AVEC FREDERIC MAURO (1998)

International audience
LY53857, a 5HT2 Receptor Antagonist, Delays Occlusion and Inhibits Platelet Aggregation in a Rabbit Model of Carotid Artery Occlusion (1991)

The present study was designed to evaluate the effectiveness of the ergoline 5HT2 receptor antagonist, LY53857 in a rabbit model of vascular arterial occlusion. LY53857 (1 and 10 microM) inhibited serotonin amplified platelet aggregation responses to threshold concentrations of ADP in rabbit platelets in vitro. LY53857 (1 microM) not only inhibited the serotonin component of rabbit platelet aggregation, but also inhibited in vitro aggregation induced by ADP (48.7 +/- 16.7% inhibition), collagen (76.1 +/- 15.9% inhibition) and U46619 (65.2 +/- 12.3% inhibition). The effectiveness of this ergoline 5HT2 receptor antagonist in blocking aggregation to ADP, collagen and U46619 may be related to its ability to inhibit a serotonin component of platelet aggregation since rabbit platelets possess high concentrations of serotonin that may be released during aggregation produced by other agents. Based on the effectiveness of LY53857 to inhibit rabbit platelet aggregation, we explored the ability of LY53857 to extend the time to carotid artery occlusion in rabbits following electrical stimulation of the artery. Reproducible carotid artery occlusion was induced in rabbits by moderate stenosis coupled to arterial cross clamping, followed by electrical stimulation. With this procedure, occlusion occurred at 47.0 +/- 7 min (n = 30) after initiation of the electrical stimulation. Animals pretreated with LY53857 (50 to 500 micrograms/kg i.v.) showed a delay in the time to carotid artery occlusion (at 100 micrograms/kg i.v. occlusion time extended to 164 +/- 16 min). Furthermore, ex vivo platelet aggregation from animals treated with LY53857 (300 micrograms/kg i.v.) resulted in 40.5% inhibition of platelet aggregation in response to the combination of ADP (1 microM) and serotonin (1 microM).(ABSTRACT TRUNCATED AT 250 WORDS)
Fast Algorithms for Interactive Coding (2013)

Consider two parties who wish to communicate in order to execute some interactive protocol π. However, the communication channel between them is noisy: An adversary sees everything that is transmitted over the channel and can change a constant fraction of the bits as he pleases, thus interrupting the execution of π (which was designed for an errorless channel). If π only contained one message, then a good error correcting code would have overcame the noise with only a constant overhead in communication, but this solution is not applicable to interactive protocols with many short messages. Schulman (FOCS 92, STOC 93) presented the notion of interactive coding: A simulator that, given any protocol π, is able to simulate it (i.e. produce its intended transcript) even with constant rate adversarial channel errors, and with only constant (multiplicative) communication overhead. Until recently, however, the running time of all known simulators was exponential (or sub-exponential) in the communication complexity of π (denoted N in this work). Brakerski and Kalai (FOCS 12) recently presented a simulator that runs in time poly(N). Their simulator is randomized (each party flips private coins) and has failure probability roughly 2−-N. In this work, we improve the computational complexity of interactive coding. While at least N computational steps are required (even just to output the transcript of π), the BK simulator runs in time . We present two efficient algorithms for interactive coding: The first with computational complexity O(N log N) and exponentially small failure probability; and the second with computational complexity O(N), but failure probability 1/poly(N). (Computational complexity is measured in the RAM model.)
Model-based fault diagnosis using fuzzy matching (1997)

A new fuzzy-model-based approach to fault detection and diagnosis is proposed. The scheme uses a set of fuzzy reference models which describe faulty and fault-free operation, and a classifier based on fuzzy matching for fault diagnosis. The reference models are obtained off-line from simulation data. A fuzzy model which describes the actual behavior of the plant is identified online from normal operating data and compared to each of the reference models. A degree of similarity is evaluated every time the online fuzzy model is identified. Dempster's rule of combination is used to combine new evidence with that already collected. The method of diagnosis accounts for any ambiguity (which may result from fault-free and faulty operation, or different faults, having similar symptoms at a given operating point) by comparing the fuzzy reference models with each other. Results are presented which demonstrate the effectiveness of the scheme when it is used to detect and identify faults in the cooling coil subsystem of the air-handling unit of both simulated and experimental air-conditioning plant.
Titelbild: Angew. Chem. 15/2002 (2002)

Das Titelbild zeigt das Innere einer rotfigurigen Trinkschale aus der Zeit 440–430 v. Chr. (British Museum, London), auf der die großen Taten des Theseus, eines Helden der griechischen Mythologie, abgebildet sind. In der Bildmitte schleppt Theseus den getöteten Minotaurus aus dem Labyrinth, rechts erhebt er die Hand gegen Skeiron mit seiner menschenfressenden Schildkröte. Im Uhrzeigersinn sind weiter der Fang des Stieres von Marathon, die Bestrafung des Sinnis, das Erschlagen des Schweins Phäa, der Kampf gegen Kerkyon und schließlich die Bestrafung des Prokrustes dargestellt. Die Mühen des Theseus unterscheiden sich kaum von denen heutiger Synthesechemiker, die auf dem Gebiet der Totalsynthese arbeiten. Ein solches Abenteuer, die Totalsynthese der CP-Moleküle mit ihren Herausforderungen, ihren Windungen und Wendungen und Sackgassen, aber auch mit ihrem Lohn, stellen K. C. Nicolaou und P. S. Baran in ihrem Aufsatz auf S. 2800 ff. der Suche nach dem Minotaurus gegenüber.
Platinized Chloroplasts: A Novel Photocatalytic Material (1985)

Colloidal platinum was prepared and precipitated directly onto photosynthetic thylakoid membranes from aqueous solution and entrapped on fiberglass filter paper. This composition of matter was capable of sustained simultaneous photoevolution of hydrogen and oxygen when irradiated at any wavelength in the chlorophyll absorption spectrum. Experimental data support the interpretation that part of the platinum metal catalyst is precipitated adjacent to the photosystem I reduction site of photosynthesis and that electron transfer occurs across the interface between photosystem I and the catalyst. Photoactivity of the material was dependent on the nature of the ionic species from which the platinum was precipitated. All photoactive samples were prepared from the hexachloroplatinate(IV) ion, whereas samples prepared by precipitation of the tetraammineplatinum(II) ion showed no hydrogen evolution activity and only transient oxygen activity. This system is among the simplest known for photosynthetically splitting water into molecular hydrogen and oxygen.
Soluble haemoglobin scavenger receptor (sCD163) in patients with suspected community‐acquired infections (2006)

The aim of our study was to evaluate soluble haemoglobin scavenger receptor (sCD163) as a molecular marker in patients with community-acquired infections. One hundred and ninety-four adult patients admitted to the Department of Internal Medicine, Odense University Hospital, with suspected community-acquired infection were included in a prospective study. Plasma and serum were sampled from all patients on day of admission and sCD163 and interleukin-6 levels were measured. Demographic data, co-morbidity, microbiological aetiology, biochemical parameters, focus of infection, severity score and mortality on day 28 were recorded. Median age was 68 (range 18-92) years. Mortality rate among infected patients on day 28 was 3.8%. sCD163 concentrations (median and range) were: 2.99 mg/l (1.22-12.65) in non-infected patients, 3.62 mg/l (1.59-74.04) (p=0.08) in infected patients without systemic inflammatory response syndrome, 3.2 mg/l (0.54-22.51) (p=0.4) in patients with sepsis, 3.63 mg/l (1.71-28.4) (p=0.01) in patients with severe sepsis, and 4.9 mg/l (2.66-28.4) (p=0.003) in patients with bacteraemia. In this cohort dominated by mild infections, a moderate elevation of sCD163 was observed only in patients with severe sepsis and/or bacteraemia. sCD163 did not discriminate between infected and non-infected patients.
Tuning the lower critical solution temperature of thermoresponsive polymers by biospecific recognition (2011)

A thermosensitive statistical copolymer based on oligo(ethylene glycol) methacrylates incorporating biotin was synthesized by free radical copolymerisation. The influence of added avidin on its thermoresponsive behaviour was investigated. The specific binding of avidin to the biotinylated copolymers provoked a marked increase of the lower critical solution temperature.
Interictal Spikes Precede Ictal Discharges in an Organotypic Hippocampal Slice Culture Model of Epileptogenesis (2010)

In organotypic hippocampal slice cultures, principal neurons form aberrant excitatory connections with other principal cells in response to slicing induced deafferentation, similar to mechanisms underlying epileptogenesis in posttraumatic epilepsy. To investigate the consequences of this synaptogenesis, the authors recorded field-potential activity from area CA3 during perfusion with the complete growth medium used during incubation. At 7 days in vitro, slice cultures only displayed multiunit activity. At 14 days in vitro, the majority displayed population bursts reminiscent of interictal-like spikes, but sustained synchronous activity was rare. Band-pass filtering of interictal discharges revealed fast ripple-like complexes, similar to in vivo recordings. Spontaneous ictal-like activity became progressively more prevalent with age: at 21 days in vitro, 50% of organotypic hippocampal slice cultures displayed long-lasting, ictal-like discharges that could be suppressed by phenytoin, whereas interictal activity was not suppressed. The fraction of cultures displaying ictal events continually increased with incubation time. Quantification of population spike activity throughout epileptogenesis using automatic detection and clustering algorithms confirmed the appearance of interictal-like activity before ictal-like discharges and also revealed high-frequency pathologic multiunit activity in slice cultures at 14 to 17 days in vitro. These experiments indicate that interictal-like spikes precede the appearance of ictal-like activity in a reduced in vitro preparation. Epileptiform activity in cultures resembled in vivo epilepsy, including sensitivity to anticonvulsants and steadily increasing seizure incidence over time, although seizure frequency and rate of epileptogenesis were higher in vitro. Organotypic hippocampal slice cultures comprise a useful model system for investigating mechanisms of epileptogenesis as well as developing antiepileptic and antiepileptogenic drugs.
A simple bounded disorder file organization with good performance (1988)

A bounded-disorder (BD) file is one in which data are organized into nodes that are indexed, e.g., by means of a B-tree. The data nodes are multibucket nodes that are accessed by hashing. In this paper we present two important improvements to the BD organization as originally described. First, records in a data node that overflow their designated primary bucket are stored in a single overflow bucket which is itself a bucket of the data node. Second, when file space needs to be increased, partial expansions are used that employ elastic buckets. Analysis and simulation results demonstrate that this variant of the BD organization has utilization, random access performance, and file growth performance that can be competitive with good extendible hashing methods, while supporting high-performance sequential access. The simplicity of the organization results in simple algorithms for realizing the organization.
Use of a Genetic Algorithm to Analyze Robust Stability Problems (1991)

This note resents a genetic algorithm technique for testing the stability of a characteristic polynomial whose coefficients are functions of unknown but bounded parameters. This technique is fast and can handle a large number of parametric uncertainties. We also use this method to determine robust stability margins for uncertain polynomials. Several benchmark examples are included to illustrate the two uses of the algorithm. 27 refs., 4 figs.
Field-theoretic simulations of directed self-assembly in cylindrical confinement: placement and rectification aspects (2014)

We have investigated the directed self-assembly (DSA) of cylinder-forming block copolymers inside cylindrical guiding templates. To complement and corroborate our experimental study, we use field-theoretic simulations to examine the fluctuations-induced variations in the size and position of the cylindrical microdomain that forms in the middle of the guiding hole. Our study goes beyond the usual mean-field approximation and self-consistent field theory simulations (SCFT) and incorporates the effects of thermal fluctuations in the description of the self-assembly process using complex Langevin (CL) dynamics. In both our experimental and modeling efforts, we focus on minor-block-attractive sidewalls and bottom substrates and neutral top surfaces and explore the properties of the formed cylinders, including fluctuations in the center position and the size of the domain, for various prepattern conditions. Our results indicate robust critical dimensions (CD) of the DSA cylinders relative to the incoming CD, with a sigma CD < 0.9nm. Likewise, we find that the DSA cylinders are accurately registered in the center of the guiding hole, with deviations in the hole-inhole distance on the order of ≈ 0.7-1nm, translating to errors in the hole-to-hole distance of ≈ 1-1.5nm.