gebhart/patrepeval huggingface dataset:
Activity of herpes simplex virus type 1-specified glycoprotein C antigenic site II epitopes reversibly modulated by peripheral fucose or galactose units of glycoprotein oligosaccharides (1990)

We have previously shown that the herpes simplex virus type 1 (HSV-1)-specified glycoprotein C (gC-1) produced in epitheloid cells contains epitopes of peptide nature, which are dependent on galactose of oligosaccharides for their expression. In the present communication we report that these epitopes are expressed in a mouse neuroblastoma cell line (C1300) with low levels of galactosyl transferases. However, in place of galactose the glycoprotein from C1300 cells was found to contain oligosaccharides with additional fucose units. Fucosidase treatment, but not galactosidase treatment, abolished the antigenic activity of the carbohydrate-dependent epitopes. Altogether the results indicated that the carbohydrate-dependent epitopes of gC-1 from C1300 cells were stabilized by peripheral sugars of N-linked oligosaccharides rather than O-linked ones and that fucose could substitute for terminal galactose in promoting the activity of the carbohydrate-dependent epitopes. This is the first demonstration of the involvement of fucose in the establishment of a carbohydrate-dependent epitope of peptide nature. The results also demonstrated that reversible carbohydrate-peptide interactions were responsible for the activity of the carbohydrate-dependent epitopes.
PHTHALIDE FORMATION: IV. CONDENSATIONS WITH 5-METHOXY-3-METHYLBENZOIC ACID (1963)

The products of the condensation of 5-methoxy-3-methylbenzoic acid with aqueous formaldehyde and hydrochloric acid in the presence of glacial acetic acid are dependent on the length of the heating period. A very short period of heating (2 minutes) yields 6-methoxy-4-methylphthalide and the two chloromethylphthalides of m.p. 135° and 154 °C, related to the unisolated 4-methoxy-6-methylphthalide. If the period of heating is 20 minutes or longer, the above chloromethylphthalide melting at 135° is formed and in addition some of the 6-methoxy-4-methylphthalide is chloromethylated to 6-methoxy-7-chloromethyl-4-methylphthalide, melting at 179°. The orientation of all three chloromethylphthalides has been established.
Maraviroc: A coreceptor CCR5 antagonist for management of HIV infection (2009)

Available data support the use of maraviroc, the first CCR5 antagonist to receive FDA marketing approval, as part of an optimized antiretroviral regimen in treatment-experienced patients infected with CCR5-tropic HIV.
Crystal Structure and Activity of Human p23, a Heat Shock Protein 90 Co-chaperone (2000)

p23 is a co-chaperone for the heat shock protein, hsp90. This protein binds hsp90 and participates in the folding of a number of cell regulatory proteins, but its activities are still unclear. We have solved a crystal structure of human p23 lacking 35 residues at the COOH terminus. The structure reveals a disulfide-linked dimer with each subunit containing eight beta-strands in a compact antiparallel beta-sandwich fold. In solution, however, p23 is primarily monomeric and the dimer appears to be a minor component. Conserved residues are clustered on one face of the monomer and define a putative surface region and binding pocket for interaction(s) with hsp90 or protein substrates. p23 contains a COOH-terminal tail that is apparently less structured and is unresolved in the crystal structure. This tail is not needed for the binding of p23 to hsp90 or to complexes with the progesterone receptor. However, the tail is necessary for optimum active chaperoning of the progesterone receptor, as well as the passive chaperoning activity of p23 in assays measuring inhibition of heat-induced protein aggregation.
A TDMA indoor radio communications system using cyclical slow frequency hopping and coding-experimental results and implementation issues (2003)

The authors describe the principle, implementation, and performance of an experimental 1.5-GHz radio communications system within a medium-size office building. The system features 1-Mb/s TDMA (time division multiple access) for service flexibility and slow frequency hopping and coding for immunity against multipath fading and interference. Measurements show that with a hallway-mounted distributed antenna system and with the mobile unit transmitting only 1 mW of peak RF power, only a single 384-bit frame out of a total of about 200,000 transmitted frames suffered an unrecoverable error. The same excellent performance was obtained from a central antenna, but only with the transmitted power increased to 100 mW.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Inhibition of Gastric H<sup>+</sup>,K<sup>+</sup>-ATPase and<i>Helicobacter pylori</i>Growth by Phenolic Antioxidants of<i>Curcuma amada</i> (2007)

Gastric ulcer is the most prevalent gastrointestinal disorder, resulting from oxidative stress, Helicobacter pylori infection, up-regulation of proton potassium ATPase (PPA) activity, down-regulation of gastric mucosal defense, etc. In this paper it is reported that phenolic fractions of Curcuma amada, commonly known as mango ginger, acted as potent inhibitors of PPA and H. pylori growth. Mango ginger free phenolics (MGFP) and mango ginger bound phenolics (MGBP) inhibited PPA at IC50 values of 2.2 +/- 0.21 and 0.7 +/- 0.08 microg/mL, respectively, exhibiting 9-27-fold better potency over lansoprazole (IC(50) of 19.3 +/- 2.2 microg/mL). MGFP is constituted by caffeic (26%), gentisic (24%), ferulic (20%), gallic (10%), cinnamic (7%), and protocatechuic acids (7%) and MGBP by ferulic (47%), cinnamic (29%), p-coumaric acid (11%), and syringic (5%) acids as major phenolic acids. MGFP and MGBP further exhibited free radical scavenging (IC(50) of 2.2 +/- 0.17 and 4.2 +/- 0.36 microg/mL), reducing power abilities (193-104 units/g), inhibition of lipid peroxidation (IC(50) of 10.3 +/- 0.91 and 15.6 +/- 1.6 microg/mL), and DNA protection (80% at 4 microg), indicating strong antioxidative properties. MGFP and MGBP thus may be potential and inexpensive multistep blockers against ulcers.
Advanced Man-machine Interfaces And Control Architecture For Dexterous Teleoperations (2005)

will perform complex tasks under challenging operational constraints. Important applications include platform servicing, payload deployment-and-retrieval, underseas salvage, and telescience -- all of which are casually structured, non-repetitive activities that require good perception, planning, and control dexterity in the face of changing environmental conditions. For the foreseeable future, teleoperations, versus automation, will be the achievable system solution. Significant technical innovation and experimentation is needed to overcome control problems arising from data rate limitations, time delay, mobile platform and contact dynamics; further, operator workloads and training requirements must be reduced to viable levels. We have been developing a telerobotic system compvting architecture and supporting man-machine interfaces to address a number of these issues, as envisioned for future space servicing, construction, and science scenarios, Examples of work to date include: 1) 3-D graphic simulators for task preview, training, andprediction; 2) a distributed “local-remote” telemanipulation control architecture and supporting operator interfaces that enable dexterous dual-arm manipulation in position, force, rate, and compliance modes, also, flexible viewing; 3) computer-assists for shared task control and force control stabilization under time delay. We survey the key technical developments in this work, illustrating our progress with experimental results taken from generic laboratory tasks. We also describe our related laboratory telerobotic simulation of the 1984 NASA Solar Maximum Satellite EVA Repair mission. Ongoing work in support of the latter includes development of a new dual eight d.0.f. arm telemanipulator, design of a hierarchically structured graphics user interface allowing rapid on-line selection and parameterization of control modes, and a virtual task environment for high-fidelity stereo graphics task preview with simulated contact forces. Underseas robotics, like its space counterpart,
Self-Development Properties of Nitrocellulose (Dependence on Ion Energies) (1989)

The dependence of self-development properties of nitrocellulose on ion energy was investigated in order to clarify the mechanism for the self-development of nitrocellulose. Experimental results were studied comparing the calculated electronic and nuclear stopping powers and projected range. It was concluded from the study that it was difficult to remove nitrocellulose film through self-development when it was irradiated with light ions such as He + and Ne + which have small nuclear stopping powers. As such, irradiation with ions which have sufficient nuclear stopping power, such as Ar + , is required in order to completely remove nitrocellulose through self-development.
Fast modelling of the collimator–detector response in Monte Carlo simulation of SPECT imaging using the angular response function (2005)

Interactions of incident photons with the collimator and detector, including septal penetration, scatter and x-ray fluorescence, are significant sources of image degradation in applications of SPECT including dual isotope imaging and imaging using radioisotopes that emit high- or medium-energy photons. Modelling these interactions using full Monte Carlo (MC) simulations is computationally very demanding. We present a new method based on the use of angular response functions (ARFs). The ARF is a function of the incident photon's direction and energy and represents the probability that a photon will either interact with or pass through the collimator, and be detected at the intersection of the photon's direction vector and the detection plane in an energy window of interest. The ARFs were pre-computed using full MC simulations of point sources that include propagation through the collimator-detector system. We have implemented the ARF method for use in conjunction with the SimSET/PHG MC code to provide fast modelling of both interactions in the patient and in the collimator-detector system. Validation results in the three cases studied show that there was good agreement between the projections generated using the ARF method and those from previously validated full MC simulations, but with hundred to thousand fold reductions in simulation time.
Water expandable polystyrene (WEPS): Part 4. Synthesis of the water expandable blend of polystyrene and poly(2,6- dimethyl-1,4-phenylene ether) (2006)

Abstract In previous studies, we discussed the use of water as an environmentally benign blowing agent for foaming of polystyrene beads, resulting in so-called WEPS, viz. water expandable polystyrene foam. Several grades of precursors to WEPS have been prepared by varying the water content and the molar mass, to control the melt strength of the polystyrene matrix. Additionally, we have used (endcapped) poly(2,6-dimethyl-1,4-phenylene ether) (PPE) to control both the meltviscosity and the glass transition temperature Tg. The advantage of using the blend PS/PPE is that the processing window for foaming is increased in view of the difference between the Tg and the boiling point of water. Using the novel WEPS route with some adjustments, for the first time a water expandable homogeneous blend of polystyrene and end-capped PPE was successfully obtained.
Fully updatable three-dimensional holographic stereogram display device based on organic monolithic compound (2013)

Holographic technique is a unique method to reproduce object on a device in three dimensions (3D). It allows us real 3D images with full parallax without special eye glasses or any special optical devices. we present fully updatable holographic 3D display system using a holographic stereographic technique with a transparent optical device of poly(methylmethacrylate) doped organic compound of 3-[(4-nitrophenyl)azo]-9H-carbazole-9-ethanol (NACzE). 100 elemental holograms which are a series of pictures of object took from different angles can completely reproduce updatable entire hologram of object. Former hologram of object can be over-recorded and immediately replaced by new hologram of object without erasing process. Typical recording time for an elemental hologram is 200 ms, and total recording time including translational stage movement for 100 elemental holograms is 28 s. The present system with preferred memory is a good candidate for 3D signage application.
Superhard nanocomposite coatings. From basic science toward industrialization (2002)

Abstract A variety of superhard coatings with Vickers plastic hardness exceeding 40 GPa have been reported by several research groups during the last five years (for recent reviews see refs [1,2]). However, one has to distinguish between superhard nanocomposites, such as nc-TiN/a-Si3N4, nc-TiN/a-Si3N4/a- and nc-TiSi2, nc-(Ti1-xAlx)N/a-Si3N4, nc-TiN/TiB2, nc-TiN/BN, etc. where the high hardness originates from the nanostrucutre and, therefore, remains stable upon annealing to high temperatures [1], and coatings, such as CrN/Ni, ZrN/Ni, and others [2] in which the measured high hardness is due to a high compressive stress that is induced in the coatings due to energetic ion bombardment during their deposition (e.g., by magnetron sputtering). We also summarize the recent progress in the industrial applications of the superhard nanocomposite coatings on machining tools.
Spectral studies on the deoxyribonucleic acid-aflatoxin B<sub>1</sub> system. Binding interactions (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleSpectral studies on the deoxyribonucleic acid-aflatoxin B1 system. Binding interactionsW. C. Neely, J. A. Lansden, and J. R. McDuffieCite this: Biochemistry 1970, 9, 8, 1862–1866Publication Date (Print):April 1, 1970Publication History Published online1 May 2002Published inissue 1 April 1970https://pubs.acs.org/doi/10.1021/bi00810a029https://doi.org/10.1021/bi00810a029research-articleACS PublicationsRequest reuse permissionsArticle Views61Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Editing the Epigenome: Reshaping the Genomic Landscape (2018)

The eukaryotic epigenome has an instrumental role in determining and maintaining cell identity and function. Epigenetic components such as DNA methylation, histone tail modifications, chromatin accessibility, and DNA architecture are tightly correlated with central cellular processes, while their dysregulation manifests in aberrant gene expression and disease. The ability to specifically edit the epigenome holds the promise of enhancing understanding of how epigenetic modifications function and enabling manipulation of cell phenotype for research or therapeutic purposes. Genome engineering technologies use highly specific DNA-targeting tools to precisely deposit epigenetic changes in a locus-specific manner, creating diverse epigenome editing platforms. This review summarizes these technologies and insights from recent studies, describes the complex relationship between epigenetic components and gene regulation, and highlights caveats and promises of the emerging field of epigenome editing, including applications for translational purposes, such as epigenetic therapy and regenerative medicine.
Clathrin-coated vesicle assembly polypeptides: physical properties and reconstitution studies with brain membranes. (1988)

The assembly polypeptides are an integral component of coated vesicles and may mediate the linkage of clathrin to the vesicle membrane. We have purified assembly polypeptides in milligram quantities from bovine brain by an improved procedure. Hydrodynamic and chemical crosslinking studies indicate that the protein is an asymmetric heterotetramer with a molecular weight of 252,000, containing two subunits of Mr 98,000-115,000, one subunit of 52,000, and one subunit of 16,000. Two-dimensional peptide maps of the subunits show that the 16- and 52-kD polypeptides are not derived from the higher molecular weight species, and that the group of bands at 98-115 kD are related. Electron microscopic visualization shows an essentially globular protein with one or two knob-like tails. We demonstrate a specific membrane protein binding site for 125I-labeled assembly polypeptides in 0.1 N sodium hydroxide-extracted bovine brain membranes based on the following criteria: (a) binding is displaceable by unlabeled ligand, (b) the binding site is destroyed by protease treatment of the membranes, and (c) the distribution of binding between vesicle-depleted membranes and coated vesicle membranes parallels the in vivo localization of assembly polypeptides and clathrin. This binding site is likely to be an integral membrane protein because (a) it is enriched in the sodium hydroxide-extracted membranes stripped of most of their peripheral membrane proteins, and (b) the binding site is partially extracted by 0.5% Triton X-100. A similar binding site appears to be present in coated vesicles. Clathrin binds to the hydroxide-stripped membranes in an assembly polypeptides dependent manner, and this binding is diminished by Triton extraction of the membranes. This assay may aid in identification of the membrane receptor for the assembly polypeptides.
Attenuation of lightning‐produced sferics in the Earth‐ionosphere waveguide and low‐latitude ionosphere (2013)

We compare radio atmospherics (sferics) detected by the World Wide Lightning Location Network (WWLLN) to very low frequency (VLF) whistler waves observed in the low‐latitude ionosphere by the Vector Electric Field Instrument of the Communications/Navigation Outage Forecasting System (C/NOFS) satellite. We also model the propagation of these sferics through the Earth‐ionosphere waveguide to the subsatellite point using the Long‐Wavelength Propagation Capability software and compare this result to the same C/NOFS data set. This unprecedentedly expansive data set allows comparison to theory and prior observation of VLF radio wave propagation in the Earth‐ionosphere waveguide and low‐latitude ionosphere. We show that WWLLN and C/NOFS observe the well‐known effect of variable attenuation with direction within the Earth‐ionosphere waveguide. Propagation within the ionosphere is also examined, and a lack of attenuation above 400 km is observed. Finally, in comparison to recent works using Detection of Electro‐Magnetic Emissions Transmitted from Earthquake Regions (DEMETER) data by Fiser et al. and Chum et al., we find that C/NOFS successfully detects whistlers with comparable amplitudes at much greater distances, compared to those reported for DEMETER.
Synthesis, structural determination and dynamic behavior of 2-chloro-4,6-bis(pyrazolylamino)-1,3,5-triazines (2003)

A series of 2-chloro-4,6-bis(pyrazolylamino)-1,3,5-triazines with applications in crystal engineering have been prepared. At low temperature, the presence of two or three isomers has been detected and these assigned to 4,6-diamino-1,3,5-triazine structures on the basis of comparison with model compounds. 2D-Exchange spectroscopy studies in various solvents and at different temperatures have been used to determine the equilibrium constants and the activation free energies of the restricted rotation about the amino-triazine bond. A plot of the activation free energy versus temperature showed a good linear correlation and confirmed that the same process is present in all of the compounds under investigation. Comparison with model compounds also confirmed both the occurrence of the restricted rotation and the 4,6-diamino-1,3,5-triazine tautomerism for triazines 1-4 in solution. The structure of compound 1 has been determined in the solid state by X-ray crystallography and consists ofa 4,6-diamino- 1,3,5-triazine structure stabilized by intra and intermolecular hydrogen bonds.
Extracellular DNA traps are associated with the pathogenesis of TRALI in humans and mice (2012)

Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related death. The biologic processes contributing to TRALI are poorly understood. All blood products can cause TRALI, and no specific treatment is available. A "2-event model" has been proposed as the trigger. The first event may include surgery, trauma, or infection; the second involves the transfusion of antileukocyte antibodies or bioactive lipids within the blood product. Together, these events induce neutrophil activation in the lungs, causing endothelial damage and capillary leakage. Neutrophils, in response to pathogens or under stress, can release their chromatin coated with granule contents, thus forming neutrophil extracellular traps (NETs). Although protective against infection, these NETs are injurious to tissue. Here we show that NET biomarkers are present in TRALI patients' blood and that NETs are produced in vitro by primed human neutrophils when challenged with anti-HNA-3a antibodies previously implicated in TRALI. NETs are found in alveoli of mice experiencing antibody-mediated TRALI. DNase 1 inhalation prevents their alveolar accumulation and improves arterial oxygen saturation even when administered 90 minutes after TRALI onset. We suggest that NETs form in the lungs during TRALI, contribute to the disease process, and thus could be targeted to prevent or treat TRALI.
Detachable-Fingered Hands for Manipulation of Large Internal Organs in Laparoscopic Surgery (2007)

In laparoscopic surgery, surgeons can use only rod-shaped instruments that can be inserted through a trocar and therefore high technique is required. To overcome this problem, much work has been done to develop multi-degree-of-freedom forceps. However they are still inadequate to grasp, manipulate or push aside large internal organs, like a spleen, pancreas, and liver. This paper proposes mechanical hands with detachable fingers that can be assembled in the abdominal cavity, each of whose parts can be inserted through a trocar. Two types of such three-fingered hands are developed. The three fingers of one are dependently driven and those of the other are independently driven. For each hand, we show fixing and power transmission mechanisms. Power of each hand is transmitted from operator's hand to its fingers by connecting wires with a ball and socket. Experimental results verify that both hands can grasp large and oily objects like internal organs. One goal of this study is to develop simple hands that can be used as a retractor. The other goal is to develop skillful hands that can replace surgeons' hands in hand assisted laparoscopic surgery which is still invasive by making a 7-8cm incision to insert a human hand into the abdominal cavity.