gebhart/patrepeval huggingface dataset:
Human Elongator facilitates RNA polymerase II transcription through chromatin (2002)

A human Elongator complex was purified from HeLa cells and found to be composed of three polypeptides. Human Elongator contains histone acetyltransferase activity with specificity to histone H3 and, to a much lesser extent, to histone H4. Although many reports have suggested a role for the yeast Elongator in transcription elongation through chromatin templates, no direct evidence supporting this function exists. In the present study, we demonstrate that the human Elongator facilitates transcription by RNA polymerase II in a chromatin- and acetyl-CoA-dependent manner. The complex was found to directly interact with RNA polymerase II but failed to interact with other factors that facilitated RNA polymerase II to traverse through nucleosomes. From our results, we postulate that different mechanisms operate to ensure efficient transcription by RNA polymerase II on chromatin templates.
Optimal bridge maintenance planning based on probabilistic performance prediction (2004)

Current automated maintenance planning procedures for deteriorating bridges are usually based on deterministic prediction of bridge performance and whole-life maintenance costing. In these procedures, uncertainties associated with the deterioration process under no maintenance and under maintenance are not taken into consideration. In this paper, such uncertainties are confined to the parameters that define the selected computational models and their effects are evaluated by means of Monte Carlo simulations. A multiobjective genetic algorithm based numerical procedure is used to locate, in the Pareto optimal sense, the best possible tradeoff maintenance planning solutions with respect to three objective functions, namely, condition index, safety index, and cumulative life-cycle maintenance cost. By computing these objectives in terms of either sample mean or sample percentile values, bridge managers’ specific confidence levels on the performance of maintenance solutions can therefore be conveniently incorporated into the optimization process.
Automatic functional analysis of left ventricle in cardiac cine MRI. (2013)

The proposed technique automatically and rapidly detects endocardial, epicardial, papillary muscles' and trabeculations' contours providing accurate and reproducible quantitative MRI parameters, including LV mass and EF.
CHL1 Is a Dual-Affinity Nitrate Transporter of Arabidopsis Involved in Multiple Phases of Nitrate Uptake (1999)

Higher plants have both high- and low-affinity nitrate uptake systems. These systems are generally thought to be genetically distinct. Here, we demonstrate that a well-known low-affinity nitrate uptake mutant of Arabidopsis, chl1, is also defective in high-affinity nitrate uptake. Two to 3 hr after nitrate induction, uptake activities of various chl1 mutants at 250 microM nitrate (a high-affinity concentration) were only 18 to 30% of those of wild-type plants. In these mutants, both the inducible phase and the constitutive phase of high-affinity nitrate uptake activities were reduced, with the inducible phase being severely reduced. Expressing a CHL1 cDNA driven by the cauliflower mosaic virus 35S promoter in a transgenic chl1 plant effectively recovered the defect in high-affinity uptake for the constitutive phase but not for the induced phase, which is consistent with the constitutive level of CHL1 expression in the transgenic plant. Kinetic analysis of nitrate uptake by CHL1-injected Xenopus oocytes displayed a biphasic pattern with a Michaelis-Menten Km value of approximately 50 microM for the high-affinity phase and approximately 4 mM for the low-affinity phase. These results indicate that in addition to being a low-affinity nitrate transporter, as previously recognized, CHL1 is also involved in both the inducible and constitutive phases of high-affinity nitrate uptake in Arabidopsis.
Theoretical and Computational Models of Word Learning: Trends in Psychology and Artificial Intelligence (2013)

"This book combines cross-disciplinary research into one comprehensive volume to help readers gain a fuller understanding of the developmental processes and influences that makeup the progression of word learning" - Provided by publisher
Microwave Characteristics of (Zr, Sn)TiO <sub>4</sub> and BaO‐PbO‐Nd <sub>2</sub> O <sub>3</sub> ‐TiO <sub>2</sub> Dielectric Resonators (1984)

The microwave characteristics of two dielectric resonator materials were investigated. This research included (Zr, Sn)TiO 4 , a material having the characteristics of a dielectric constant K = 38, Q = 7000 at 7 GHz, and temperature coefficient of resonant frequency τ f , = 0 ppm/°C. The investigation determined the relations between the dielectric loss and micro‐structures of this ceramic. Analysis by X‐ray microanalyzer made it clear that the addition of Fe 2 O 3 increased the dielectric loss of this ceramic because the Fe ions diffused into the grain. The other material investigated was BaO‐PbO‐Nd 2 O 3 ‐TiO 2 , a ceramic having a dielectric constant of K = 88, Q = 5000 at 1 GHz, and τ f = 0 ppm/°C. As this ceramic has a very high dielectric constant, it is useful for applications at frequencies <1 GHz.
Regulation of hematopoiesis in vitro by alloreactive natural killer cell clones. (1993)

Natural killer (NK) cells lyse autologous and allogeneic target cells even in the absence of major histocompatibility complex (MHC) class I antigens on the target cells. Recently, however, human allospecific NK cell clones have been generated that recognize at least five distinct specificities inherited recessively and controlled by genes linked to the MHC. Because the genetic specificity of these alloreactive NK cells in vitro appears analogous to that of in vivo NK cell-mediated murine hybrid resistance, i.e., the rejection of parental bone marrow in irradiated F1 animals, we tested the ability of human alloreactive NK clones to recognize allogeneic hematopoietic progenitor cells. NK cells from two specificity 1 alloreactive NK clones, ES9 and ES10, significantly and often completely suppressed colony formation by purified peripheral blood hematopoietic progenitor cells from specificity 1-susceptible donors, but had no significant effect on the cells of specificity 1-resistant donors. Activated polyclonal NK cells were less efficient than the NK clones in inhibiting colony formation and had a similar effect on cells from both specificity 1-susceptible and -resistant donors. The alloreactive NK clones produced cytokines with a suppressive effect on in vitro hematopoiesis, such as interferon gamma (IFN-gamma) and tumor necrosis factor alpha (TNF-alpha), when exposed to phytohemagglutinin blasts from specificity 1-susceptible, but not -resistant donors. However, the mechanism by which alloreactive NK cells inhibit colony formation is more consistent with a direct cytotoxic effect than with the production of inhibitory cytokines because antibodies (anti-IFN-gamma, alpha-TNF-alpha, and -lymphotoxin) that completely blocked the inhibition by polyclonal NK cells had only a minimal effect on the inhibition by the alloreactive clones. Moreover, the alloreactive clones were directly cytolytic in a 51Cr release assay against enriched preparations of peripheral blood progenitor cells from specificity 1-susceptible donors. These data indicate that the alloreactive NK cells are likely the human counterpart of the cells mediating murine hybrid resistance and that these cells might play clinically important roles in rejection or in graft-versus-leukemia reactions after allogeneic bone marrow transplantation.
Integrated Nanostructures Based on Self-Assembled Amphiphilic Polypeptides (2017)

Nano-ordered materials are requiring on demand shape control in various fields; biomaterials, medicals, pharmaceuticals and so on. Self-assembly of molecules is a typical bottom-up phenomenon observed in nature to form nano-materials. Self-assembly leads to the formation of various structures that impart functions. We found that amphiphilic block polypeptides having helical structure as a hydrophobic block self-assemble to form different structures. First, the hydrophobic segments form helical structures that define specific structures of self-assemblies such as fibers, tubes, vesicles and sheets depending on the diameter and length of helix. Small diameter of 310-helix induced fibers and micelles reasonably due to small critical packing parameter. On the other hand, sheet-like assembly were obtained by peptide having large sectional area of α-helix. Depending on the length of hydrophobic helix, these sheets transformed into various morphology by heat treatment. Length of helix changes the thickness and size of sheet and thus, changes the flexibility of the sheet, resulting that the difference of just two residues induced drastically different shapes of assembly, tube, vesicle and sheet. Next, hydrophilic polymers, polyethylene glycol (PEG) and poly(sarcosine), didn’t affect drastically the shape but did the size of assembly owing to the difference of hydrophilicity and flexibility. Poly(sarcosine) made even small assembly of ~200 nm length and 80 nm diameter more stable and PEG enhanced the elongation of tubular assembly (more than 1 μm length) with the same diameter of 80 nm. Furthermore, we demonstrate some integration of the structures by mixing with other components such as lipids, cholesterol, or amphiphilic polypeptides having a different hydrophilic chain of polyethylene glycol or a different-length hydrophobic helix. This work shows one of the methods to prepare complex morphology of molecular assembly.
Protein Folding Failure Sets High-Temperature Limit on Growth of Phage P22 in <i>Salmonella enterica</i> Serovar Typhimurium (2004)

The high-temperature limit for growth of microorganisms differs greatly depending on their species and habitat. The importance of an organism's ability to manage thermal stress is reflected in the ubiquitous distribution of the heat shock chaperones. Although many chaperones function to reduce protein folding defects, it has been difficult to identify the specific protein folding pathways that set the high-temperature limit of growth for a given microorganism. We have investigated this for a simple system, phage P22 infection of Salmonella enterica serovar Typhimurium. Production of infectious particles exhibited a broad maximum of 150 phage per cell when host cells were grown at between 30 and 39 degrees C in minimal medium. Production of infectious phage declined sharply in the range of 40 to 41 degrees C, and at 42 degrees C, production had fallen to less than 1% of the maximum rate. The host cells maintained optimal division rates at these temperatures. The decrease in phage infectivity was steeper than the loss of physical particles, suggesting that noninfectious particles were formed at higher temperatures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a decrease in the tailspike adhesins assembled on phage particles purified from cultures incubated at higher temperatures. The infectivity of these particles was restored by in vitro incubation with soluble tailspike trimers. Examination of tailspike folding and assembly in lysates of phage-infected cells confirmed that the fraction of polypeptide chains able to reach the native state in vivo decreased with increasing temperature, indicating a thermal folding defect rather than a particle assembly defect. Thus, we believe that the folding pathway of the tailspike adhesin sets the high-temperature limit for P22 formation in Salmonella serovar Typhimurium.
Chemical transformation of uronic acids leading to aminocyclitols. III. Syntheses of aminocyclitols and aminocyclitol-oligoglycosides from uronic acids and glucuronide-saponins by means of electrolytic decarboxylation. (1981)

Conversions of uronic acids [e.g. methyl 2, 3, 4-tri-O-methyl-β-D-glucopyranosiduronic acid (1), D-glucuronic acid (9), and D-galacturonic acid (17)] into the corresponding aminocyclitols [e.g. 6b, 7b, 8b ; 14b, c, 15b, c, 16b, c ; 18b] have been accomplished by means of initial electrolytic decarboxylation and subsequent treatment with alkaline nitromethane, followed by catalytic reduction, and acetylation. The conversion method has also been applied to neutral sugar [e.g. D-mannose (19)] to affording L-neo-aminocyclitol hexaacetate (22b) and also to two glucuronide-saponins [sakuraso-saponin (23) and desacyl-jegosaponin (27)] to afford aminocyclitol-oligoglycosides (26a, 28a). The present electrolytic conversion is noteworthy since prior protection of hydroxyl groups in the starting uronic acids is not necessary.
IP development and management of IP DB enabling efficient system-on-chip design (2003)

For efficient system-on-chip (SOC) design, we need an IP DB (intellectual property database) where everyone can register IP and access IP they want. To quickly collect and register IP from companies and universities, we have to give some benefits to the IP providers while minimizing the IP standard they must observe. To provide IP suitable for IP users, we have to have an IP DB which contains rated IP and has sufficient and convenient functions such as IP search, compare and download. Finally, to satisfy the various requirements of both IP providers and IP users, we have to have many business models and license models working in reality. We present KETI's works to solve these problems, focusing on the development and management of an IP DB.
POEM-an office system for international use (2003)

A description is given of a versatile office system called POEM (Paperless Office and Electronic Mail). This system runs on PCs as well as on mini- and microcomputers. It has been designed for compatibility with the international standardization activities of ISO/CCITT within the area of office environments, i.e. information and communication technologies. The power of POEM is enhanced by its multilingual user interface, the possibility of handling multilingual documents in all standardized formats, and its support of communication services in accord with international standards.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Calcium pentosan polysulfate directly inhibits enzymatic activity of ADAMTS4 (aggrecanase‐1) in osteoarthritic chondrocytes (2008)

Aggrecanases that include ADAMTS1, 4, 5, 8, 9 and 15 are considered to play key roles in aggrecan degradation in osteoarthritic cartilage. Here we demonstrate that calcium pentosan polysulfate (CaPPS) directly inhibits the aggrecanase activity of ADAMTS4 without affecting the mRNA expression of the ADAMTS species in interleukin-1alpha-stimulated osteoarthritic chondrocytes. Synthetic peptides corresponding to specific regions of the thrombospondin type 1 repeat, cysteine-rich or spacer domain of ADAMTS4 inhibit the binding to immobilized CaPPS. These data suggest that CaPPS could function as chondroprotective agent for the treatment of osteoarthritis by inhibition of ADAMTS4 through interaction with the C-terminal ancillary domain.
Glucocorticoids and Chromosomal Position Modulate Murine Mammary Tumor Virus Transcription by Affecting Efficiency of Promoter Utilization (1983)

The rate of transcription of murine mammary tumor virus (MTV) sequences in MTV-infected rat hepatoma tissue culture cells is strongly affected by both glucocorticoid hormones and the chromosomal position of provirus integration. We have characterized MTV RNAs produced in J2.17 and M1.54, independent isolates containing, respectively, 1 and 10 proviruses integrated at distinct chromosomal loci. M1.54, but not J2.17, synthesized MTV RNA in the absence of glucocorticoids; the rate of hormone-stimulated viral gene transcription in M1.54 was 50- to 100-fold higher than in J2.17. In each case in which MTV genes were expressed (J2.17 induced, M1.54 basal and induced), the viral RNAs produced were indistinguishable. RNA blotting revealed accumulation of two transcripts, 7.8 and 3.8 kilobases; the latter was likely produced from the former by RNA splicing. Sites used for transcription initiation, polyadenylation, and splicing have been identified from the sizes of end-labeled hybridization probes protected from digestion with mung bean nuclease; the unique initiation and polyadenylation sites were both encoded within the MTV long-terminal-repeat sequence. The efficiencies of splicing and of utilization of the polyadenylation signal did not appear to vary as functions of chromosomal position or hormonal stimulation. Differences in rates of viral gene transcription were reflected in the differential accumulation of the 5'-terminal 136 nucleotides of MTV RNA. Thus, glucocorticoids and chromosomal position appeared to affect solely the efficiency of utilization of the MTV promoter, leaving unchanged the sites of initiation, splicing, and polyadenylation, as well as the efficiencies of the latter two processes.
Fault modeling of differential ECL (1995)

The paper deals with testability analysis of differential ECL. The logic behaviour and the drop in performance concerning a very detailed list of possible defects of a high speed ECL-Design was examined. The test circuit, an AND gate (bandwidth: dc to 3.4 Gb/s), was designed taking into account a low power consumption and a small overhead as it is used for weighted random pattern generation and signature analysis (edge counting) within a Built-In-Self-Test (BIST)-Architecture. It was realized using a 1.2 /spl mu/m bipolar technology. It is shown that defects in differential ECL devices may cause redundant or delay faults in respect to operation speed.
Separation and purification of lithium by solvent extraction and supported liquid membrane, analysis of their mechanism: a review (2016)

Abstract Separation and purification of lithium by solvent extraction and supported liquid membrane using various commercial and non‐commercial extraction has been reviewed. In solvent extraction, extraction by chelating extractants, acidic extractants, solvation extractants and mechanism involved in the extraction has been discussed. Solvent extraction of lithium by solvation extractants like; TOPO, DBM, TBP, and LIX and synergism of diketone with various solvation extractant combinations have been reviewed. Finally, lithium extraction by supported liquid membrane using various extractants has been reviewed. The reported processes mainly lacking in analysis of chemical mechanism involved in the lithium extraction using all these extractants are analyzed and discussed. © 2016 Society of Chemical Industry
Optical properties of thin semiconductor device structures with reflective back-surface layers (1999)

Ultrathin semiconductor device structures incorporating reflective internal or back surface layers have been investigated recently as a means of improving photon recuperation eliminating losses associated with free carrier absorption in conductive substrates and increasing the above bandgap optical thickness of thermophotovoltaic device structures. However, optical losses in the form of resonance absorptions in these ultrathin devices have been observed. This behavior in cells incorporating epitaxially grown FeAl layers and in devices that lack a substrate but have a back-surface reflector (BSR) at the rear of the active layers has been studied experimentally and modeled effectively. For thermophotovoltaic devices, these resonances represent a significant loss mechanism since the wavelengths at which they occur are defined by the active TPV cell thickness of ∼2–5 microns and are in a spectral range of significant energy content for thermal radiators. This study demonstrates that ultrathin semiconductor structures that are clad by such highly reflective layers or by films with largely different indices of refraction display resonance absorptions that can only be overcome through the implementation of some external spectral control strategy. Effective broadband below-bandgap spectral control using a back-surface reflector is only achievable using a large separation between the TPV active layers and the back-surface reflector.
Deferoxamine-induced attenuation of brain edema and neurological deficits in a rat model of intracerebral hemorrhage (2004)

Deferoxamine and other iron chelators may be potential therapeutic agents for ICH. They may act by reducing the oxidative stress caused by the release of iron from the hematoma.
When Baseball Met Big Bill Haywood: The Battle for Manchester, New Hampshire, 1912–1916 (2019)

Book Review| April 01 2019 When Baseball Met Big Bill Haywood: The Battle for Manchester, New Hampshire, 1912–1916 Roper, Scott C., and Stephanie Abbot Roper. When Baseball Met Big Bill Haywood: The Battle for Manchester, New Hampshire, 1912–1916. Jefferson, NC: McFarland, 2018. Pp. 210. Bibliography, index, and notes. $29.95, pb. Lindsay John Bell Lindsay John Bell Iowa State University Search for other works by this author on: This Site Google Journal of Sport History (2019) 46 (1): 129–130. https://doi.org/10.5406/jsporthistory.46.1.0129 Cite Icon Cite Share Icon Share Facebook Twitter LinkedIn MailTo Permissions Search Site Citation Lindsay John Bell; When Baseball Met Big Bill Haywood: The Battle for Manchester, New Hampshire, 1912–1916. Journal of Sport History 1 January 2019; 46 (1): 129–130. doi: https://doi.org/10.5406/jsporthistory.46.1.0129 Download citation file: Zotero Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All Scholarly Publishing CollectiveUniversity of Illinois PressJournal of Sport History Search Advanced Search The text of this article is only available as a PDF. Copyright 2019 by the Board of Trustees of the University of Illinois2019 Article PDF first page preview Close Modal You do not currently have access to this content.
Electrochemical method for entrapment of oligonucleotides in polymer-coated electrodes (1999)

Oligonucleotides were incorporated into conducting films of poly(3, 4-ethylene dioxythiophene) by an electrochemical method that involves two steps. The electrode is first coated with the polymer film by electropolymerization followed by electrooxidation of the formed polymer in the presence of the oligonucleotide. Neutral water soluble polymers such as poly(vinylpyrrolidone) or poly(ethylene glycol) were added to the polymerizing medium resulting in higher incorporation yields of oligonucleotides. The results showed that a payload of about 1.5 nmol/cm2 can be achieved in an 8 micron thick film for an oligomer concentration of about 70 microM in the working solution. Various physical methods were used to analyze the surface of the polymer-coated electrodes. Results showed the presence of domains of varying sizes at the film surface, corresponding to the insertion of the hydrophilic polymer within the matrix of the conductive polymer. The release of entrapped oligonucleotides from the coated film exhibited a three-step profile: a "burst" period during the first 5 min followed by an intermediate and a very slow release period lasting several days. Such polymer films could be exploited as useful reservoirs of biologically active substances for in vivo delivery to targeted tissues. For example, coated stents that can release antiproliferative agents such as antisense oligonucleotides at the site of balloon dilatation may be of interest in the treatment of postangioplasty restenosis.
Ignition and combustion of powdered metals in the atmospheres of Venus, earth, and Mars. (1965)

Ignition temperatures of powdered metals in simulated atmospheres of Venus and Mars suggest potential chemical energy sources
Real-Time PCR Detection of <i>gyrA</i> and <i>parC</i> Mutations in <i>Streptococcus pneumoniae</i> (2008)

Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.