gebhart/patrepeval huggingface dataset:
Evidence for alpha‐melanocyte‐stimulating hormone (α‐MSH) receptors on human malignant melanoma cells (1988)

The presence of alpha-MSH receptors on human melanoma has so far been suggested in the literature but not proved. We describe a reproducible and specific binding assay of alpha-MSH on human melanoma cells, using a high-specific-activity 125I-labelled hormone (1.5 to 2 mCi/micrograms) with consistent receptor binding (usually exceeding 2 pg/10(6) cells) and stable for 3 weeks. Asynchronized cells in suspension were incubated for 15 min at 37 degrees C with the tracer and various concentrations of unlabelled hormones. Synthetic alpha-MSH was compared to beta-MSH, ACTH1-24, ACTH4-10, beta-LPH, CLIP, CRF, MIF I, A8VP and beta-endorphin. Out of a panel of 8 human melanoma cell lines, 3 showed specific and reproducible alpha-MSH binding curves. No significant binding to human fibroblast and human carcinoma cells was seen. alpha-MSH, beta-MSH and, to a lesser extent ACTH4-10 (a part of the alpha-MSH sequence) were the only peptides able to displace labelled alpha-MSH from its binding sites, indicating the high specificity of the MSH receptor. Affinity constants (Ka) ranged from 10(8) to 10(9) l/mole and the estimated receptor number was 1,000 to 2,000 per cell. We conclude that some human melanoma cell lines expressed specific MSH receptors with stable affinity but which are low in number.
Exfoliated Nanosheets as a New Strong Solid Acid Catalyst (2003)

Two-dimensional metal oxide sheets in HTiNbO(5) and HSr(2)Nb(3)O(10), cation-exchangeable layered metal oxides, were examined as solid acid catalysts. Exfoliation of HTiNbO(5) and HSr(2)Nb(3)O(10) in aqueous solutions formed colloidal single-crystal TiNbO(5)(-) and Sr(2)Nb(3)O(10)(-) nanosheets, which precipitated under an acidic condition to form aggregates of HTiNbO(5) nanosheets and HSr(2)Nb(3)O(10) nanosheets. Although esterification of acetic acid, cracking of cumene, and dehydration of 2-propanol were not catalyzed by original HTiNbO(5) because of the narrow interlayer distance, which prevents the insertion of organic molecules, HTiNbO(5) nanosheets functioned as a strong solid acid catalyst for the reactions. Nanosheets of HSr(2)Nb(3)O(10) exhibited no or slight catalytic activity for these reactions. NH(3) temperature-programmed desorption and (1)H magic-angle spinning nuclear magnetic resonance spectroscopy revealed that HTiNbO(5) nanosheets have strong Brønsted acid sites, whereas HSr(2)Nb(3)O(10) nanosheets do not.
Integration of computer aided design and computer aided manufacturing using STEP data exchange standard (2016)

This paper proposes shop-floor programming system for integration of CAD and CAM for 3D prismatic parts having different features like slot, pocket, hole etc. The major modules of the proposed methodology are (i) Modeling module (ii) Interpreter module (iii)Automatic Feature Recognition (AFR) module (iv)Process planning module (v) Part Program generation module. In this paper, an object oriented approach has been used to model the 3D prismatic part in VC++ using ST-Developer software package. The part model is based on STEP AP 203, the resulting part 21 file is interpreted and converted in to a Parasolid, which is given has an input to the AFR module where the manufacturing view software is used to recognize the features automatically. By using workingstep list obtained from AFR module, machining time is calculated for each operation and an automatic process sequence has been generated with alternative sequence of operations having different machining times. The sequence which is having lowest machining time is given input to the commercial CAM system to generate part program.
Web-based tool for estimating the environmental impacts of telework (2002)

This paper describes a WWW-based decision support tool for analyzing the environmental and economic impacts of telework over a set of likely parameters and scenarios, exposing crucial system components and sensitivities. It is expected that the analysis provided by this web tool will enable commuters, employees, company managers, and regulators to gain a better understanding of telework-related environmental impacts.
Deep Fake Image Detection Based on Pairwise Learning (2020)

Generative adversarial networks (GANs) can be used to generate a photo-realistic image from a low-dimension random noise. Such a synthesized (fake) image with inappropriate content can be used on social media networks, which can cause severe problems. With the aim to successfully detect fake images, an effective and efficient image forgery detector is necessary. However, conventional image forgery detectors fail to recognize fake images generated by the GAN-based generator since these images are generated and manipulated from the source image. Therefore, in this paper, we propose a deep learning-based approach for detecting the fake images by using the contrastive loss. First, several state-of-the-art GANs are employed to generate the fake–real image pairs. Next, the reduced DenseNet is developed to a two-streamed network structure to allow pairwise information as the input. Then, the proposed common fake feature network is trained using the pairwise learning to distinguish the features between the fake and real images. Finally, a classification layer is concatenated to the proposed common fake feature network to detect whether the input image is fake or real. The experimental results demonstrated that the proposed method significantly outperformed other state-of-the-art fake image detectors.
Spatial separation of endothelial small‐ and intermediate‐conductance calcium‐activated potassium channels (K<sub>Ca</sub>) and connexins: possible relationship to vasodilator function? (2006)

Activation of endothelial cell small- (S) and intermediate- (I) conductance calcium-activated potassium channels (K(Ca)) and current or molecular transfer via myoendothelial gap junctions underlies endothelium-derived hyperpolarization leading to vasodilation. The mechanism underlying the K(Ca) component of vasodilator activity and the characteristics of gap junctions are targets for the selective control of vascular function. In the rat mesenteric artery, where myoendothelial gap junctions and connexin (Cx) 40 are critical for the transmission of the endothelial cell hyperpolarization to the smooth muscle, SK(Ca) and IK(Ca) provide different facets of the endothelium-derived hyperpolarization response, being critical for the hyperpolarization and repolarization phases, respectively. The present study addressed the question of whether this functional separation of responses may be related to the spatial localization of the associated channels? The distribution of endothelial SK(Ca) and IK(Ca) and Cx subtype(s) were examined in the rat mesenteric artery using conventional confocal and high-resolution ultrastructural immunohistochemistry. At the internal elastic lamina-smooth muscle cell interface at internal elastic lamina holes (as potential myoendothelial gap junction sites), strong punctate IK(Ca), Cx37 and Cx40 expression was present. SK(Ca), Cx37, Cx40 and Cx43 were localized to adjacent endothelial cell gap junctions. High-resolution immunohistochemistry demonstrated IK(Ca) and Cx37-conjugated gold to myoendothelial gap junction-associated endothelial cell projections. Clear co-localization of K(Ca) and Cxs suggests a causal relationship between their activity and the previously described differential functional activation of SK(Ca) and IK(Ca). Such precise localizations may represent a selective target for control of vasodilator function and vascular tone.
Comparison of novel cannabinoid partial agonists and SR141716A in the guinea‐pig small intestine (2000)

The controversial nature of the CB(1) receptor antagonist, SR141716A, in the guinea-pig small intestine was investigated by comparing it with four analogues of Delta(8)-tetrahydrocannabinol (Delta(8)-THC): O-1184, O-1238, O-584 and O-1315. These compounds (10 - 1000 nM) inhibited the electrically-evoked contractions with a rank order of potency of O-1238>O-1184>O-584>O-1315. Log concentration-response curves for O-1238, O-1184 and O-1315 were significantly shifted to the right by SR141716A and the maxima were significantly less than that of the CB(1) agonist, WIN55212-2, an indication of partial agonism. Partial saturation of the triple bond in O-1184 to a cis double bond (O-1238) increased its potency as an agonist (pEC(50) from 6.42 to 7.63) and as an antagonist of WIN55212-2, (pK(B), from 8.36 to 9.49). Substitution of the terminal azide group by an ethyl group (O-584) or removal of the phenolic hydroxyl group (O-1315) had no significant effect on the agonist or antagonist potency. None of these analogues increased the twitch response in a manner resembling that of SR141716A. O-1184 (10 and 100 nM) shifted the log concentration-response curve of WIN55212-2 for inhibition of the twitch responses to the right with pK(B) values of 8.29 and 8.38, respectively. We conclude that these Delta(8)-THC analogues behave as partial agonists rather than silent antagonists at CB(1) binding sites in this tissue. There was no evidence of antagonism of endocannabinoids thus supporting the hypothesis that, in this tissue, SR141716A is an inverse agonist of constitutively active CB(1) receptors.
An Inverse Model for Seasonal Circulation over the Southern Brazilian Shelf: Near-Surface Velocity from the Heat Budget (1998)

An inverse model was developed of the seasonal upper-layer circulation in the region adjacent to the coast of southern Brazil between 30° and 35°S, 50° and 55°W near the boundary of the Brazil–Malvinas confluence. In this region there are no in situ data on circulation, while a large array of data on sea surface temperature and surface heat fluxes acquired through the last 30 years is available. The model is based on the joint analysis of observed seasonal changes of SST along with observed fields of insolation and surface heat fluxes. From the equation of heat conservation integrated over the variable depth of the mixed layer the “residual” flux fields required to explain the observed temperature changes between seasons were computed. Generally, this residual flux accounts for advection, diffusion, and entrainment. Diffusion has been neglected based on scaling estimates of term sizes, while entrainment was parameterized through observed changes of the mixed layer depth, with the assumption that the temperature in the seasonal thermocline was linear in depth. The remaining “residual flux” must be associated with advection. In this way, the seasonal fields of surface advection were constructed. The fields of advection together with the fields of SST determine the cross-isotherm components of velocity fields, while the along-isotherm components are in the “null space” of the inversion. However, given the cross-isotherm components, along-isotherm components can be determined from the equation of continuity, with the condition of no motion at the rigid coastal boundary. Following this scheme, total near-surface velocity fields were computed for each season on a regular grid (20′ × 20′) in the chosen region. Results suggested that in all seasons the coastal part of the region is dominated by northward flow, probably related to the alongshore spreading of waters discharged from the Plata estuary. The offshore part of the region is involved in the southward motion associated with the Brazil Current. The impact of some specific dynamical features of the region, such as bottom topography and water discharge from the Lagoa dos Patos, is also evident in the model results.
Supervised learning with decision tree-based methods in computational and systems biology (2009)

At the intersection between artificial intelligence and statistics, supervised learning allows algorithms to automatically build predictive models from just observations of a system. During the last twenty years, supervised learning has been a tool of choice to analyze the always increasing and complexifying data generated in the context of molecular biology, with successful applications in genome annotation, function prediction, or biomarker discovery. Among supervised learning methods, decision tree-based methods stand out as non parametric methods that have the unique feature of combining interpretability, efficiency, and, when used in ensembles of trees, excellent accuracy. The goal of this paper is to provide an accessible and comprehensive introduction to this class of methods. The first part of the review is devoted to an intuitive but complete description of decision tree-based methods and a discussion of their strengths and limitations with respect to other supervised learning methods. The second part of the review provides a survey of their applications in the context of computational and systems biology.
Thermal and flicker phase noise analysis in rotary traveling‐wave oscillator (2019)

Summary This paper analyzes the thermally induced phase noise and the up‐conversion of flicker noise into phase noise of rotary traveling‐wave oscillator (RTWO). Based on the analyses, this paper extracts the closed‐form formulas for the thermal and flicker phase noise of the RTWO. This paper compares the theoretical results with appropriate simulations to evaluate the accuracy of the derived closed‐form formulas. Comparisons confirm the accuracy of the extracted phase noise formulas. By using the presented straightforward approach along with accurate phase noise formulas, the designers can understand the RTWO ' s design tradeoffs. Also, they can design the RTWO for a specific phase noise without needing lengthy simulations.
Adaptive control of surviving symbol replica candidates in QRM-MLD for OFDM MIMO multiplexing (2006)

This paper proposes adaptive control of the number of surviving symbol replica candidates, S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> (m denotes the stage index), based on the minimum accumulated branch metric of each stage in maximum-likelihood detection employing QR decomposition and the M-algorithm (QRM-MLD) in orthogonal frequency-division multiplexing with multiple-input-multiple-output (MIMO) multiplexing. In the proposed algorithm, S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> at the mth stage (1lesmlesN <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> , N <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">t</sub> is the number of transmission antenna branches) is independently controlled using the threshold value calculated from the minimum accumulated branch metric at that stage and the estimated noise power. We compared the computational complexity of QRM-MLD employing the proposed algorithm with that of conventional methods at the same average packet error rate assuming the information bit rate of 1.048 Gb/s in a 100-MHz channel bandwidth (i.e., frequency efficiency of approximately 10 bit/s/Hz) using 16QAM modulation and turbo coding with the coding rate of 8/9 in 4-by-4 MIMO multiplexing. Computer simulation results show that the average computational complexity of the branch metrics, i.e., squared Euclidian distances, of the proposed adaptive independent S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> control method is decreased to approximately 38% that of the conventional adaptive common S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> control and to approximately 30% that of the fixed S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> method (S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</sub> =M=16), assuming fair conditions such that the maximum number of surviving symbol replicas at each stage is set to Mcirc=16
INFLUENCE OF DOSE AND ROUTE OF ANTIGEN INJECTION ON THE IMMUNOLOGICAL INDUCTION OF T CELLS (1974)

Delayed-type hypersensitivity (DTH) develops in the absence of an adjuvant when mice are injected intravenously or subcutaneously with an appropriate dose of sheep red blood cells (SRBC). The optimal intravenous dose of 10(5) SRBC (in CD-1 mice) produces maximum DTH which decays exponentially from its peak on day 4. Increasing the dose of SRBC reduces and eventually abolishes all evidence of DTH. DTH fails to reappear in respose to secondary stimulation except in splenectomized mice in whom the development of DTH is not suppressed, even by massive doses of SRBC. Hence the suppression cannot be due to antigen as such. The optimal dose of SRBC for sensitization by footpad inoculation is 100-fold higher (10(7) SRBC in CD-1 mice), but even 10(9) SRBC do not block the induction of DTH by this route of immunization. A blocking dose of SRBC, given intravenously 1 day before footpad inoculation, completely suppresses cell proliferation in the draining lymph node, prevents PFC production there, and blocks the induction of DTH by a sensitizing dose of SRBC. If given 1 day after footpad sensitization, intravenous antigen has little effect on the cellular response in the regional node but DTH is still completely suppressed. Blocking of induction and expression may depend, therefore, on different mechanisms.
Identification of a Novel Toll‐like Receptor 7 Endogenous Ligand in Rheumatoid Arthritis Synovial Fluid That Can Provoke Arthritic Joint Inflammation (2015)

The results of this study underscore the importance of miR-let-7b ligation to TLR-7 in the joint during the effector phase of RA.
Electrochemical oxidation of cholesterol (2015)

Indirect cholesterol electrochemical oxidation in the presence of various mediators leads to electrophilic addition to the double bond, oxidation at the allylic position, oxidation of the hydroxy group, or functionalization of the side chain. Recent studies have proven that direct electrochemical oxidation of cholesterol is also possible and affords different products depending on the reaction conditions.
Image-based nanocrystallography with online database support (2006)

The crystallographic phase and morphology of many materials change with the crystal size so that new needs arise to determine the crystallography of nanocrystals. Direct space high-resolution phase-contrast transmission electron microscopy (HRTEM) and atomic resolution scanning TEM (STEM) when combined with tools for image-based nanocrystallography in two (2D) and three (3D) dimensions possess the capacity to meet these needs. After a concise discussion of lattice-fringe visibility spheres and maps, this paper discusses lattice-fringe fingerprinting in 2D and tilt protocol applications. On-line database developments at Portland State University (PSU) that support image-based nanocrystallography are also mentioned.
Very Small Embryonic-Like Stem Cells: Biology and Therapeutic Potential for Heart Repair (2011)

Very small embryonic-like stem cells (VSELs) represent a population of extremely small nonhematopoietic pluripotent cells that are negative for lineage markers and express Sca-1 in mice and CD133 in humans. Their embryonic-like characteristics include the expression of markers of pluripotency; the ability to give rise to cellular derivatives of all three germ-layers; and the ability to form embryoid-like bodies. Indeed, quiescent VSELs may represent the remnants of epiblast-derived cells in adult organs. After tissue injury, including acute myocardial infarction (MI), bone marrow-derived VSELs are mobilized into the peripheral blood and home to the damaged organ. Given the ability of VSELs to differentiate into cardiomyocytes and endothelial cells, and their ability to secrete various cardioprotective growth factors/cytokines, VSELs may serve as an ideal cellular source for cardiac repair. Consistently, transplantation of VSELs after an acute MI improves left ventricular (LV) structure and function, and these benefits remain stable during long-term follow-up. Although the mechanisms remain under investigation, effects of secreted factors, regeneration of cellular constituents, and stimulation of endogenous stem/progenitors may play combinatorial roles. The purpose of this review is to summarize the current evidence regarding the biologic features of VSELs, and to discuss their potential as cellular substrates for therapeutic cardiac repair.
Interacting with hidden content using content-aware free-space transparency (2004)

We present <i>content-aware free-space transparency</i>, an approach to viewing and manipulating the otherwise hidden content of obscured windows through unimportant regions of overlapping windows. Traditional approaches to interacting with otherwise obscured content in a window system render an entire window uniformly transparent. In contrast, content-aware free-space transparency uses opaque-to-transparent gradients and image-processing filters to minimize the interference from overlapping material, based on properties of that material. By increasing the amount of simultaneously visible content and allowing basic interaction with otherwise obscured content, without modifying window geometry, we believe that free-space transparency has the potential to improve user productivity.
Hybrid upconversion nanomaterials for optogenetic neuronal control (2015)

Nanotechnology-based approaches offer the chemical control required to develop precision tools suitable for applications in neuroscience. We report a novel approach employing hybrid upconversion nanomaterials, combined with the photoresponsive ion channel channelrhodopsin-2 (ChR2), to achieve near-infrared light (NIR)-mediated optogenetic control of neuronal activity. Current optogenetic methodologies rely on using visible light (e.g. 470 nm blue light), which tends to exhibit high scattering and low tissue penetration, to activate ChR2. In contrast, our approach enables the use of 980 nm NIR light, which addresses the short-comings of visible light as an excitation source. This was facilitated by embedding upconversion nanomaterials, which can convert NIR light to blue luminescence, into polymeric scaffolds. These hybrid nanomaterial scaffolds allowed for NIR-mediated neuronal stimulation, with comparable efficiency as that of 470 nm blue light. Our platform was optimized for NIR-mediated optogenetic control by balancing multiple physicochemical properties of the nanomaterial (e.g. size, morphology, structure, emission spectra, concentration), thus providing an early demonstration of rationally-designing nanomaterial-based strategies for advanced neural applications.
Recommended tests for association in 2×2 tables (2009)

The asymptotic Pearson's chi-squared test and Fisher's exact test have long been the most used for testing association in 2x2 tables. Unconditional tests preserve the significance level and generally are more powerful than Fisher's exact test for moderate to small samples, but previously were disadvantaged by being computationally demanding. This disadvantage is now moot, as software to facilitate unconditional tests has been available for years. Moreover, Fisher's exact test with mid-p adjustment gives about the same results as an unconditional test. Consequently, several better tests are available, and the choice of a test should depend only on its merits for the application involved. Unconditional tests and the mid-p approach ought to be used more than they now are. The traditional Fisher's exact test should practically never be used.