gebhart/patrepeval huggingface dataset:
Lymphohemopoietic reconstitution using wheat germ agglutinin-positive hemopoietic stem cell transplantation within but not across the major histocompatibility antigen barriers. (1993)

Nonadherent (NA), low density (LD), wheat germ agglutinin-positive (WGA+) murine hemopoietic stem cell-enriched preparations (HSCPs) were tested for the capability to reconstitute lymphohemopoietic elements in lethally irradiated mice. HSCPs from BALB/c mice reconstituted lethally irradiated, major histocompatibility complex (MHC)-matched DBA/2 mice to normal histology of the thymus and spleen and normal humoral and cellular immune functions. By contrast, lethally irradiated B6 mice could not be reconstituted after transplantation with NA, LD, WGA+ cells from MHC-mismatched BALB/c mice. We previously observed frequent survival, stable chimerism, and normally vigorous functioning immune systems in B6 mice transplanted with T-cell-depleted bone marrow from both BALB/c and B6 donors. To extend these findings to a stem cell transplantation system, lethally irradiated B6 mice were transplanted with NA, LD, WGA+ cells from both BALB/c and B6 mice. These mixed stem cell-enriched preparations did not reconstitute the lethally irradiated, MHC-mismatched mice. By contrast, such HSCPs from BALB/c plus DBA/2 into DBA/2 mice reconstituted the hematologic and lymphoid tissues and functional immune systems when the donor and the recipient pairs were matched at MHC and mismatched at multiminor histocompatibility barriers. These purified blood progenitors thus appear to lack certain cells/factors essential for engraftment and reconstituting recipients in a fully allogeneic environment.
La fouille du dolmen de l'Ustau-du-Loup (Puy-de-Dôme) (1964)

Gilbert Louis. La fouille du dolmen de l'Ustau-du-Loup (Puy-de-Dôme). In: Revue archéologique du Centre, tome 3, fascicule 3, 1964. pp. 262-269.
Isolation and characterization of new lignin streams derived from extractive-ammonia (EA) pretreatment (2016)

One of the key challenges facing lignin conversion to fuels and chemicals is related to the level of carbohydrate and ash impurities found in extracted lignin.
Patterns of loss of heterozygosity at loci from chromosome arm 13q suggest a possible involvement of <i>BRCA2</i> in sporadic breast tumors (1995)

Loss of heterozygosity (LOH) at loci from chromosome 13 is frequently observed in breast cancer. Chromosome 13 contains at least two cancer genes, the well-characterized RB1 gene located at 13q14 and the breast cancer-susceptibility gene, BRCA2, recently localized to 13q12. To investigate the possible involvement of BRCA2 in sporadic breast tumors, we looked at LOH at eight microsatellite (CA)n markers distributed along chromosome 13 in a panel of 59 primary breast carcinomas. We show that some LOH does not include the RB1 locus and is associated with the BRCA2 gene region.
Human Attribute Recognition by Rich Appearance Dictionary (2013)

We present a part-based approach to the problem of human attribute recognition from a single image of a human body. To recognize the attributes of human from the body parts, it is important to reliably detect the parts. This is a challenging task due to the geometric variation such as articulation and view-point changes as well as the appearance variation of the parts arisen from versatile clothing types. The prior works have primarily focused on handling geometric variation by relying on pre-trained part detectors or pose estimators, which require manual part annotation, but the appearance variation has been relatively neglected in these works. This paper explores the importance of the appearance variation, which is directly related to the main task, attribute recognition. To this end, we propose to learn a rich appearance part dictionary of human with significantly less supervision by decomposing image lattice into overlapping windows at multiscale and iteratively refining local appearance templates. We also present quantitative results in which our proposed method outperforms the existing approaches.
A connection admission control algorithm for low Earth orbit satellite networks (2003)

In this paper, a connection admission control algorithm, referred to as the geographical connection admission control (GCAC) algorithm, is introduced to handle the spot-beam handovers in the low Earth orbit (LEO) satellite networks. Spotbeam handovers occur frequently because of the small spotbeams and high satellite speed. The GCAC algorithm admits a new call into the network only if the expected handover call blocking probability of the new and the existing calls is not increased beyond a system threshold. The algorithm utilizes the deterministic satellite movement pattern and the user locations to estimate the expected handover call blocking probability. The experimental results show that the algorithm limits the handover call blocking probability and can handle nonuniform traffic distribution over the coverage area of the network.
Measurement of Inhibin and an Index of Inhibin Production by Rat Testes during Postnatal Development1 (1986)

Our previous studies have shown that inhibin activity in rat testes can be measured using an in vitro inhibin bioassay. In animals that have undergone unilateral efferent duct ligation (EDL), the difference in inhibin content between the ligated and nonligated testis can be used as an index of the rate of inhibin production in vivo. In the present study we examined postnatal changes in inhibin content in the testes, and the production rates of inhibin and seminiferous tubule fluid in groups of neonatal, immature, and adult rats from 1 to 80 days old. We detected inhibin activity in testes of 1-day-old rats; the activity level rose linearly to Day 8, subsequently increasing markedly from Day 30 until it reached a plateau at Day 45. Increments in the content of inhibin and weight of the testis after unilateral EDL, interpreted to represent production of inhibin and seminiferous tubule fluid, commenced at Day 20 and increased rapidly between Days 30 and 50, decreasing thereafter to Day 80. The increase in content and production of inhibin was directly correlated to the rise in serum follicle-stimulating hormone (FSH) and testosterone, suggesting that these two hormones are important in controlling inhibin secretion. In addition, the changes in serum FSH from the high, postnatal levels to those typical of adults were inversely correlated to the content and production rate inhibin in the testes and concentrations of testosterone in the serum. These data support the hypothesis that inhibin is specifically involved in the feedback control of pituitary FSH secretion during postnatal development, although a role for testosterone or a synergistic interaction between the two hormones cannot be excluded.
Docetaxel in breast cancer and a rationale for combination therapy. (1997)

Development of the taxoids has progressed rapidly in the 1990s. In vitro studies have demonstrated that docetaxel (Taxotere) has a longer residence time and higher accumulation within tumor cells than paclitaxel (Taxol), possibly accounting for its greater cytotoxicity. Animal studies have shown docetaxel to possess high antitumor activity. In clinical studies, docetaxel as a single agent has been shown to be highly active against a variety of solid tumors. It is at least as active in metastatic disease as other agents currently used, and it has demonstrated effectiveness against tumors resistant to anthracyclines or paclitaxel. Docetaxel shows some activity in cell lines resistant to fluorouracil (5-FU), vincristine, cisplatin (Platinol), and etoposide (VePesid). Its unique mechanism of action and lack of cross-resistance or overlapping toxicities with many agents may also contribute to its efficacy and safety in combination therapy.
Microencapsulation I. Phase Separation or Coacervation (1978)

AbstractPhase separation or coacervation is one of the oldest and perhaps the most widely used method of microencapsulation. This paper discusses phase separation or coacervation as a technique of preparing microcapsules. The basic differences between simple and complex coacervation are described and the various factors which influence simple and complex coacervation are discussed. Schematic ternary phase diagrams involved in the phase separation or coacervation processes are included and the basic schemes describing the process of microencapsulation are outlined.
Photolysis of HNCO vapor in the presence of NO and O<sub>2</sub> (1968)

The effect of NO at pressures up to 380 Torr on the photolysis of HNCO vapor (4.5 Torr) by light from an unfiltered medium-pressure mercury lamp has been studied at 37 °C. The yield of CO was reduced to about half its original value, which is attributed to reactions of NH and NCO with NO. The yield of N 2 showed a steady increase with increasing NO pressure, probably due to reactions of NO(A 2 ∑ + ) excited by absorption of light. The hydrogen yield fell rapidly to zero as the NO pressure was increased.Added oxygen at pressures up to 5 Torr had no effect on the CO and N 2 production at 37 °C; at 110 °C the CO yield decreased somewhat while the N 2 remained unaffected. These observations suggest that NH and NCO do not react readily with O 2 . The production of H 2 fell rapidly to zero with increasing O 2 pressure at both temperatures. The suppression of H 2 formation by both NO and O 2 supports the belief that it is formed by reactions of hydrogen atoms.
Evaluation of Formic-Acid-Based Hydrogen Storage Technologies (2014)

To make hydrogen a feasible energy carrier, its transformation into another chemical is advisible. Formic acid may constitute an attractive option to store hydrogen in a dense and safe form. The efficiency of formic-acid-based process chains for the storage of hydrogen energy has been evaluated. The efficiency is highly dependent upon the way formic acid is produced. Options based on reactions of hydrogen with carbon dioxide show a rather poor energetic performance, while routes using renewable production of intermediate carbon monoxide can reach competitive efficiencies. For the storage of electrical energy, an overall efficiency of about 12% is possible using the current state of the art technology, but alternative processes show potential for higher efficiencies.
Description d'un nouveau genre et de deux nouvelles espèces d'Ascalaphes afrotropicaux (Neuroptera, Ascalaphidae) (1998)

Michel Bruno. Description d'un nouveau genre et de deux nouvelles espèces d'Ascalaphes afrotropicaux (Neuroptera, Ascalaphidae). In: Bulletin de la Société entomologique de France, volume 103 (1), mars 1998. pp. 45-50.
R-Pulse Wave Arrival Timing with Cardiac Diseases in Pregnancy (1993)

Pulse wave arrival time (PWAT) can be measured noninvasively by using the R-wave of the electrocardiogram and the peripheral pressure pulse. This report describes our preliminary observations in the use of this physiologic parameter to study a group of 12 pregnant patients with a history of cardiac disease. Their PWATs were compared to those recorded from normal, uncomplicated pregnant women, which were reported previously. All patients were clinically stable and without signs or symptoms of congestive heart failure. In seven patients the PWAT was either normal or shortened; the latter occurred in three patients whose pregnancies were complicated by hypertension. The PWAT was prolonged in all five cases with positive laboratory evidence of cardiac compromise. These data suggest that the PWAT may be a useful screening adjunct in the diagnosis and management of early cardiac dysfunction, but does not supplant the need for conventional cardiac evaluation methods.
If you build it they might stay (2011)

We analyze mechanisms of player retention and commitment in massively multiplayer online games. Our ground assumptions on player retention are based on a marketing model of customer retention and commitment. To measure the influence of gameplay, in-game sociality, and real-life status on player commitment, we use the following metrics: weekly play time, stop rate and number of years respondents have been playing the game. The cross-cultural sample is composed of 2865 World of Warcraft players from North-America, Europe, Taiwan, and Hong-Kong who completed an online questionnaire. We differentiate players in terms of demographic categories including age, region, gender and marital status.
Murine Colonic Organoid Culture System and Downstream Assay Applications (2016)

Colonic organoids, three-dimensional colonic crypts grown in vitro that show realistic microanatomy, have many potential applications for studying physiology, developmental biology, and pathophysiology of intestinal diseases including inflammatory bowel disease and colorectal cancer. Here, we describe detailed protocols for mouse colonic crypt isolation, organoid culture, and downstream applications. Specific culture strategies including growth factor enriched Matrigel and Wnt and R-spondin conditioned media serve as key factors for enhancing the growth and cost efficiency of colonic organoid cultures.
Flexi Vector Cloning (2008)

A protocol for ligation-dependent cloning using the Flexi Vector method in a 96-well format is described. The complete protocol includes PCR amplification of the desired gene to append Flexi Vector cloning sequences, restriction digestion of the PCR products, ligation of the digested PCR products into a similarly digested acceptor vector, transformation and growth of host cells, analysis of the transformed clones, and storage of a sequence-verified clone. The protocol also includes transfer of the sequence-verified clones into another Flexi Vector plasmid backbone. Smaller numbers of cloning reactions can be undertaken by appropriate scaling of the indicated reaction volumes.
Inhibition of T-cell proliferation by a MYB antisense oligomer is accompanied by selective down-regulation of DNA polymerase alpha expression. (1990)

We recently found that inhibition of MYB protein synthesis in human peripheral blood mononuclear cells (PBMC) exposed to human c-myb (designated MYB) antisense oligodeoxynucleotides prevents entry into S phase and cell proliferation. To determine the mechanism(s) by which down-regulation of human c-myb protein (MYB) synthesis interferes with DNA synthesis, we analyzed mRNA levels of DNA polymerase alpha and proliferating cell nuclear antigen (PCNA), transcripts of two genes required for DNA synthesis, in normal and leukemic T lymphocytes exposed to MYB antisense oligodeoxynucleotides. Expression of DNA polymerase alpha was inhibited both in normal T lymphocytes progressing from G0 to S phase and in exponentially growing CCRF-CEM leukemic cells, whereas expression of PCNA was inhibited only in mitogen-stimulated PBMC and remained essentially unaffected in the leukemia T-cell line. The functional link between expression of MYB and DNA polymerase alpha mRNAs was further demonstrated by analyzing DNA polymerase alpha mRNA levels in a temperature-sensitive (ts) fibroblast cell line (TK-ts13; TK is thymidine kinase) constitutively expressing human MYB mRNA driven by the simian virus 40 (SV40) promoter. In the MYB-expressing TK-ts13 cells, DNA polymerase alpha mRNA levels were unaffected following shift to the nonpermissive temperature of 39.6 degrees C, whereas in the parental line, DNA polymerase alpha mRNA levels were readily down-regulated. These findings indicate that the expression of MYB is related to that of DNA polymerase alpha in cells expressing MYB at high levels and suggest that there is a functional link between c-myb and DNA polymerase alpha mRNA expression during cell cycle progression of normal T lymphocytes.
Electron paramagnetic resonance oxygen mapping (EPROM): Direct visualization of oxygen concentration in tissue (2000)

Tissue oxygen content is a central parameter in physiology but is difficult to measure. We report a novel procedure for spatial mapping of oxygen by electron paramagnetic resonance (EPR) utilizing a spectral-spatial imaging data set, in which an EPR spectrum is obtained from each image volume element. From this data set, spatial maps corresponding to local spin density and maximum EPR spectral line amplitude are generated. A map of local EPR spectral linewidth is then computed. Because linewidth directly correlates with oxygen concentration, the linewidth image provides a map of oxygenation. This method avoids a difficulty inherent in other oxygen content mapping techniques using EPR, that is, the unwanted influence of local spin probe density on the image. We provide simulation results and data from phantom studies demonstrating the validity of this method. We then apply the method to map oxygen content in rat tail tissue and vasculature. This method provides a new, widely applicable, approach to direct visualization of oxygen concentration in living tissue. Magn Reson Med 43:804-809, 2000.
Mechanical and electrical cold bonding based on metallic nanowire surface fasteners (2012)

Mass production of surface mount devices (SMDs) relies heavily on reflow soldering and has become the cornerstone of today's electronic industry. However, the traditional reflow soldering technique is characterized by high heating temperatures, toxic solder materials and low recycling rate of SMDs. Here, we propose a new patterned structure of Au nanowire arrays named a surface fastener through which cold bonding for surface mount technology can be realized. The mechanical bonding enables normal and shear bonding strengths of more than 5 N cm(-2). Simultaneously, the parasitic resistance of a pair of surface fasteners is only approximately 2 Ω. The present technique can be performed at room temperature, thereby improving the process compatibility and reliability of SMDs. Surface fasteners based on high melting point metallic nanowires are temperature-resistant for many critical applications. In addition, bonding without solder material is positive for the recycling of rare metals in SMDs.
Photolabelling the urotensin II receptor reveals distinct agonist- and partial-agonist-binding sites (2007)

The mechanism by which GPCRs (G-protein-coupled receptors) undergo activation is believed to involve conformational changes following agonist binding. We have used photoaffinity labelling to identify domains within GPCRs that make contact with various photoreactive ligands in order to better understand the activation mechanism. Here, a series of four agonist {[Bpa1]U-II (Bpa is p-benzoyl-L-phenylalanine), [Bpa2]U-II, [Bpa3]U-II and [Bpa4]U-II} and three partial agonist {[Bpa1Pen5D-Trp7Orn8]U-II (Pen is penicillamine), [Bpa2Pen5D-Trp7Orn8]U-II and [Pen5Bpa6D-Trp7Orn8]U-II} photoreactive urotensin II (U-II) analogues were used to identify ligand-binding sites on the UT receptor (U-II receptor). All peptides bound the UT receptor expressed in COS-7 cells with high affinity (Kd of 0.3-17.7 nM). Proteolytic mapping and mutational analysis led to the identification of Met288 of the third extracellular loop of the UT receptor as a binding site for all four agonist peptides. Both partial agonists containing the photoreactive group in positions 1 and 2 also cross-linked to Met288. We found that photolabelling with the partial agonist containing the photoreactive group in position 6 led to the detection of transmembrane domain 5 as a binding site for that ligand. Interestingly, this differs from Met184/Met185 of the fourth transmembrane domain that had been identified previously as a contact site for the full agonist [Bpa6]U-II. These results enable us to better map the binding pocket of the UT receptor. Moreover, the data also suggest that, although structurally related agonists or partial agonists may dock in the same general binding pocket, conformational changes induced by various states of activation may result in slight differences in spatial proximity within the cyclic portion of U-II analogues.
Use of NLM Medical Subject Headings with the MeSH2010 Thesaurus in the PORTAL-DOORS System (2010)

The NLM MeSH Thesaurus has been incorporated for use in the PORTAL-DOORS System (PDS) for resource metadata management on the semantic web. All 25588 descriptor records from the NLM 2010 MeSH Thesaurus have been exposed as web accessible resources by the PDS MeSH2010 Thesaurus implemented as a PDS PORTAL Registry operating as a RESTful web service. Examples of records from the PDS MeSH2010 PORTAL are demonstrated along with their use by records in other PDS PORTAL Registries that reference the concepts from the MeSH2010 Thesaurus. Use of this important biomedical terminology will greatly enhance the quality of metadata content of other PDS records thus improving cross-domain searches between different problem oriented domains and amongst different clinical specialty fields.
Systematic study of GeSn heterostructure-based light-emitting diodes towards mid-infrared applications (2016)

Temperature-dependent characteristics of GeSn light-emitting diodes with Sn composition up to 9.2% have been systematically studied. Such diodes were based on Ge/GeSn/Ge double heterostructures (DHS) that were grown directly on a Si substrate via a chemical vapor deposition system. Both photoluminescence and electroluminescence spectra have been characterized at temperatures from 300 to 77 K. Based on our theoretical calculation, all GeSn alloys in this study are indirect bandgap materials. However, due to the small energy separation between direct and indirect bandgap, and the fact that radiative recombination rate greater than non-radiative, the emissions are mainly from the direct Γ-valley to valence band transitions. The electroluminescence emissions under current injection levels from 102 to 357 A/cm2 were investigated at 300 K. The monotonic increase of the integrated electroluminescence intensity was observed for each sample. Moreover, the electronic band structures of the DHS were discussed. Despite the indirect GeSn bandgap owing to the compressive strain, type-I band alignment was achieved with the barrier heights ranging from 11 to 47 meV.
IL-6 and PD-L1 antibody blockade combination therapy reduces tumour progression in murine models of pancreatic cancer (2016)

These preclinical results indicate that targeted inhibition of IL-6 may enhance the efficacy of anti-PD-L1 in PDAC.
Swarming of<i>Pseudomonas aeruginosa</i>Is Controlled by a Broad Spectrum of Transcriptional Regulators, Including MetR (2009)

Pseudomonas aeruginosa exhibits swarming motility on semisolid surfaces (0.5 to 0.7% agar). Swarming is a more than just a form of locomotion and represents a complex adaptation resulting in changes in virulence gene expression and antibiotic resistance. In this study, we used a comprehensive P. aeruginosa PA14 transposon mutant library to investigate how the complex swarming adaptation process is regulated. A total of 233 P. aeruginosa PA14 transposon mutants were verified to have alterations in swarming motility. The swarming-associated genes functioned not only in flagellar or type IV pilus biosynthesis but also in processes as diverse as transport, secretion, and metabolism. Thirty-three swarming-deficient and two hyperswarming mutants had transposon insertions in transcriptional regulator genes, including genes encoding two-component sensors and response regulators; 27 of these insertions were newly identified. Of the 25 regulatory mutants whose swarming motility was highly impaired (79 to 97%), only 1 (a PA1458 mutant) had a major defect in swimming, suggesting that this regulator might influence flagellar synthesis or function. Twitching motility, which requires type IV pili, was strongly affected in only two regulatory mutants (pilH and PA2571 mutants) and was moderately affected in three other mutants (algR, ntrB, and nosR mutants). Microarray analyses were performed to compare the gene expression profile of a swarming-deficient PA3587 mutant to that of the wild-type PA14 strain under swarming conditions. PA3587 showed 63% homology to metR, which encodes a regulator of methionine biosynthesis in Escherichia coli. The observed dysregulation in the metR mutant of nine different genes required for swarming motility provided a possible explanation for the swarming-deficient phenotype of this mutant.