gebhart/patrepeval huggingface dataset:
Possible role for peptide-oligosaccharide interactions in differential oligosaccharide processing at asparagine-107 of the light chain and asparagine-297 of the heavy chain in a monoclonal IgG1.kappa. (1984)

The carbohydrate attached at Asn-107 of the light chain of a human myeloma IgG1 kappa (Hom) was isolated and the structure determined by 1H NMR. Two oligosaccharides were found corresponding to mono- and disialylated forms of the bisected biantennary class of glycopeptides. Both structures had Fuc alpha 1-6 linked to the GlcNAc residue attached to Asn and NeuNAc residues linked alpha 2-6. Because of the unusual nature of these structures, the Asn-297 oligosaccharides of the same IgG were prepared from Fc fragments and heavy chains. Comparison of the structures of the latter glycopeptides with structures from the same site on a second human myeloma IgG1 kappa (Tem) showed them to be quite similar in that the majority of the structures were biantennary but not bisected. We suggest that the completely bisected nature of the light-chain oligosaccharides comes from a high level of activity of GlcNAc-T-III (the enzyme responsible for the attachment of the bisecting GlcNAc) in the cells producing the IgG. We suggest a mechanism for differential glycosylation between the Asn-107 and Asn-297 sites based on the stabilization of the Asn-297 oligosaccharide in a conformation with the torsional angle omega about the C5-C6 bond of the Man alpha 1-6 linkage equal to -60 degrees. It has previously been postulated that this conformation is not a substrate for GlcNAc-T-III [Brisson, J.-R., & Carver, J. P. (1983) Can. J. Biochem. 61, 1067-1078].(ABSTRACT TRUNCATED AT 250 WORDS)
Error resilient video coding with priority data classification using H.264 flexible macroblock ordering (2007)

Flexible macroblock ordering (FMO) is a new error resilience feature in the H.264 (MPEG-4 part 10) video coding standard. The paper exploits FMO to offer a new classification algorithm for prioritised video transmission. Instead of using the default mapping structures of FMO, an optimisation algorithm in which the more important macroblocks (MBs) are categorised in a separate slice group (SG), which corresponds to high-priority packets for transmission, is proposed. The importance of each MB is determined based on its eventual influence on picture quality. This is assessed by considering the fact that the successful transmission of an MB not only enhances the quality of the associated pixels, but also improves the quality of its adjacent lost MBs by improving the efficiency of error concealment. It is assumed that the network can offer a prioritised service for successful transmission of the high-priority SG. Based on this, a vulnerability factor for each MB is determined, and a certain proportion of high-vulnerability MBs at each frame to limit the impact of temporal error propagation is intra-updated. It is shown, where the proposed mapping algorithm outperforms the default mappings of the H.264 codec, that this prioritised transmission will improve the subjective and objective video quality in situations with a high probability of transmission errors.
Structure-Distribution Relationships for Metal-Labeled Myocardial Imaging Agents: Comparison of a Series of Cationic Gallium(III) Complexes with Hexadentate Bis(salicylaldimine) Ligands (1994)

A series of 10 cationic gallium(III) complexes with hexadentate bis(salicylaldimine) ligands were synthesized, characterized, radiolabeled with 67Ga, and screened in a rat model to assess their potential as 68Ga radiopharmaceuticals for imaging the heart with positron emission tomography. The tris(salicylaldimine) ligand precursors were synthesized by condensation of either bis(3-aminopropyl)ethylenediamine (BAPEN) or bis(2,2-dimethyl-3-aminopropyl)ethylenediamine (DM-BAPEN) with 3 equiv of a salicylaldehyde derivative containing alkyl, alkoxy, or alkylamino substituents in the 4, 5, or 6 position of the aromatic ring. The cationic six-coordinate gallium(III) bis(salicylaldimine) complexes were obtained by reaction of these tris(salicylaldimines) with tris(acetylacetonato)gallium(III). X-ray crystallographic confirmation of the molecular structure of Ga[(4,6-(MeO)2sal)2DM-BAPEN]+I- shows the Ga cation to adopt a pseudo-octahedral N4O2 coordination sphere with a trans configuration. All of the 67Ga complexes are lipophilic with measured octanol/water partition coefficients (P) varying from log P = 0.84 to 3.00. These 67Ga-labeled complexes are all found to exhibit significant myocardial uptake following intravenous administration to rats (ranging from 0.34 to 1.08% of the injected dose in myocardium at 1 min postinjection) combined with the desired myocardial retention of tracer.
CO-Induced apoptotic death of colorectal cancer cells by a luminescent photoCORM grafted on biocompatible carboxymethyl chitosan (2017)

A photo-active luminescent rhenium carbonyl complex namely, [Re(CO)<sub>3</sub>(phen)(pyAl)](CF<sub>3</sub>SO<sub>3</sub>) was grafted on a biocompatible carboxymethyl chitosan (CMC) matrix through Schiff base condensation reaction. The light-induced CO delivery from ReCMC has been shown to eradicate human colorectal adenocarcinoma cells (HT-29) very efficiently in a dose-dependent fashion. The onset of CO-induced apoptosis was realized by caspase-3,-7 detection aided by fluorescence confocal microscopy. ReCMC represents a unique example of a biocompatible and biodegradable antineoplastic agent that could find its use in cancer photopharmacology.
Are Mast Cells MASTers in HIV-1 Infection? (2001)

HIV-1 gp120 interacts with IgE V(H)3(+) on the surface of human basophils and mast cells (Fc epsilon RI(+) cells), acting as a viral immunoglobulin superantigen. gp120 from different clades induces mediator release from Fc epsilon RI(+) cells. gp120 also induces IL-4 and IL-13 synthesis in human basophils. The chemokine receptors CCR3 and CXCR4, which are coreceptors of HIV-1 infection, are expressed by human Fc epsilon RI(+) cells. HIV-1 Tat protein is a potent chemoattractant for basophils and lung mast cells, interacting with CCR3. Incubation of basophils with Tat protein upregulates the surface expression of the CCR3 receptor. There is evidence that human Fc epsilon RI(+) cells could be infected in vitro by M-tropic HIV-1 strains.
High-Performance Computer Modeling of the Cosmos-Iridium Collision (2009)

This paper describes the application of a new, integrated modeling and simulation framework, encompassing the space situational awareness (SSA) enterprise, to the recent Cosmos-Iridium collision. This framework is based on a flexible, scalable architecture to enable efficient simulation of the current SSA enterprise, and to accommodate future advancements in SSA systems. In particular, the code is designed to take advantage of massively parallel, high-performance computer systems available, for example, at Lawrence Livermore National Laboratory. We will describe the application of this framework to the recent collision of the Cosmos and Iridium satellites, including (1) detailed hydrodynamic modeling of the satellite collision and resulting debris generation, (2) orbital propagation of the simulated debris and analysis of the increased risk to other satellites (3) calculation of the radar and optical signatures of the simulated debris and modeling of debris detection with space surveillance radar and optical systems (4) determination of simulated debris orbits from modeled space surveillance observations and analysis of the resulting orbital accuracy, (5) comparison of these modeling and simulation results with Space Surveillance Network observations. We will also discuss the use of this integrated modeling and simulation framework to analyze the risks and consequences of future satellite collisions and to assess strategies for mitigating or avoiding future incidents, including the addition of new sensor systems, used in conjunction with the Space Surveillance
First off the mark: early seed germination (2011)

Most plant seeds are dispersed in a dry, mature state. If these seeds are non-dormant and the environmental conditions are favourable, they will pass through the complex process of germination. In this review, recent progress made with state-of-the-art techniques including genome-wide gene expression analyses that provided deeper insight into the early phase of seed germination, which includes imbibition and the subsequent plateau phase of water uptake in which metabolism is reactivated, is summarized. The physiological state of a seed is determined, at least in part, by the stored mRNAs that are translated upon imbibition. Very early upon imbibition massive transcriptome changes occur, which are regulated by ambient temperature, light conditions, and plant hormones. The hormones abscisic acid and gibberellins play a major role in regulating early seed germination. The early germination phase of Arabidopsis thaliana culminates in testa rupture, which is followed by the late germination phase and endosperm rupture. An integrated view on the early phase of seed germination is provided and it is shown that it is characterized by dynamic biomechanical changes together with very early alterations in transcript, protein, and hormone levels that set the stage for the later events. Early seed germination thereby contributes to seed and seedling performance important for plant establishment in the natural and agricultural ecosystem.
Distributed power control for throughput balancing in CDMA systems (2002)

We consider joint rate and power control in a multi-cellular CDMA system utilizing variable processing gain. We investigate the problem of optimum power allocation for sharing the capacity in a fair way in the whole network. For this, we suggest a general distributed power control algorithm which directs the powers so that a uniform throughput is obtained among the users. We show that the powers converge towards the optimal solution at a geometric rate and how the maximum achievable throughput can be estimated during the power control.
A method for the chromatographic detection of penicillins and related compounds and of penicillinase (1961)

Research Article| June 01 1961 A method for the chromatographic detection of penicillins and related compounds and of penicillinase P. H. A. Sneath; P. H. A. Sneath 1National Institute for Medical Research, Mill Hill, London, N.W. 7 Search for other works by this author on: This Site PubMed Google Scholar J. F. Collins J. F. Collins 1National Institute for Medical Research, Mill Hill, London, N.W. 7 Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1961) 79 (3): 512–514. https://doi.org/10.1042/bj0790512 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation P. H. A. Sneath, J. F. Collins; A method for the chromatographic detection of penicillins and related compounds and of penicillinase. Biochem J 1 June 1961; 79 (3): 512–514. doi: https://doi.org/10.1042/bj0790512 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1961 CAMBRIDGE UNIVERSITY PRESS1961 Article PDF first page preview Close Modal You do not currently have access to this content.
Metal–ligand multiple bonds as frustrated Lewis pairs for C–H functionalization (2012)

The concept of frustrated Lewis pairs (FLPs) has received considerable attention of late, and numerous reports have demonstrated the power of non- or weakly interacting Lewis acid-base pairs for the cooperative activation of small molecules. Although most studies have focused on the use of organic or main-group FLPs that utilize steric encumbrance to prevent adduct formation, a related strategy can be envisioned for both organic and inorganic complexes, in which "electronic frustration" engenders reactivity consistent with both nucleophilic (basic) and electrophilic (acidic) character. Here we propose that such a description is consistent with the behavior of many coordinatively unsaturated transition-metal species featuring metal-ligand multiple bonds, and we further demonstrate that the resultant reactivity may be a powerful tool for the functionalization of C-H and E-H bonds.
Carbohydrate-Binding Module of a Rice Endo-β-1,4-glycanase, OsCel9A , Expressed in Auxin-Induced Lateral Root Primordia, is Post-Translationally Truncated (2006)

We report the cloning of a glycoside hydrolase family (GHF) 9 gene of rice (Oryza sativa L. cv. Sasanishiki), OsCel9A, corresponding to the auxin-induced 51 kDa endo-1,4-beta-glucanase (EGase). This enzyme reveals a broad substrate specificity with respect to sugar backbones (glucose and xylose) in beta-1,4-glycans of type II cell wall. OsCel9A encodes a 640 amino acid polypeptide and is an ortholog of TomCel8, a tomato EGase containing a carbohydrate-binding module (CBM) 2 sequence at its C-terminus. The expression of four rice EGase genes including OsCel9A showed different patterns of organ specificity and responses to auxin. OsCel9A was preferentially expressed during the initiation of lateral roots or subcultured root calli, but was hardly expressed during auxin-induced coleoptile elongation or in seed calli, in contrast to OsCel9D, a KORRIGAN (KOR) homolog. In situ localization of OsCel9A transcripts demonstrated that its expression was specifically up-regulated in lateral root primordia (LRP). Northern blotting analysis showed the presence of a single product of OsCel9A. In contrast, both mass spectrometric analyses of peptide fragments from purified 51 kDa EGase proteins and immunogel blot analysis of EGase proteins in root extracts using two antibodies against internal peptide sequences of OsCel9A revealed that the entire CBM2 region was post-translationally truncated from the 67 kDa nascent protein to generate 51 kDa EGase isoforms. Analyses of auxin concentration and time course dependence of accumulation of two EGase isoforms suggested that the translation and post-translational CBM2 truncation of the OsCel9A gene may participate in lateral root development.
A framework for adaptive real-time applications (2008)

Nowadays, more and more applications require OSGi to have some forms of real-time support, which is currently very limited. The resulting ad-hoc solutions forbid standard, system-wide policies for real-time system's adaptation, and reconfiguration. In order to tackle this problem, this paper proposes a declarative real-time component model. In this model, the distinguishing real-time contract of each component is declaratively described, and a general component real-time management interface is designed. They are used to maintain an accurate view of existing real-time components' promised contracts. A real-time component runtime service is designed to control the whole lifecycle of the components. By using global information and general control interface, it can adjust the system continue to operate without impairing the deployed components' real-time contracts in the face of run-time changes. This system allows itself to be easily extended with other constraint resolving policies to fit different contexts. The prototype has been tested into a simulated control system. The result shows this framework can provide good real time performance while still provides real-time component dynamicity support as well. To the best of our knowledge, this is the first comprehensive solution providing explicit real-time support from design to execution in OSGi framework.
Formation of Nickel Oxide Nanoribbons in the Cavity of Carbon Nanotubes (2001)

An attempt was made to encapsulate nickel into the cavity of the carbon nanotubes, which were prepared by the pyrolytic carbon deposition on the nanochannels of anodic aluminum oxide film. For nickel loading, the MOCVD (metal organic chemical vapor deposition) method using nickelocene was applied to the carbon-coated nanochannels and then the nickel/carbon nanotube composites were liberated from the anodic oxide film by alkaline treatment. The resultant carbon nanotubes contained numerous numbers of NiO nanoribbons with about 4 nm thickness and 20 nm width. The length of the longest nanoribbon was about 800 nm. The crystal structure of the NiO nanoribbons was determined by using the electron diffraction technique. Furthermore, the mechanism of the nanoribbon formation was discussed in relation to the formation processes of the nickel/carbon nanotube composites.
A Radio-Frequency Probe for the Mass-Spectrometric Analysis of Ion Concentrations (1955)

It has become increasingly evident of recent years that a detailed study of the processes occurring in gas discharges often requires a means of analysing and studying separately the various ionic species. This is true even in the case of the inert gases.
Phase separations in liquid crystal-colloid mixtures (2008)

We present a mean-field theory to describe phase separations in mixtures of a nematic liquid crystal and a colloidal particle. The theory takes into account an orientational ordering of liquid crystals and a crystalline ordering of colloidal particles. We calculate phase diagrams on the temperature-concentration plane, depending on interactions between a liquid crystal and a colloidal surface and a coupling between nematic and crystalline ordering. We find various phase separation processes, such as a nematic-crystal phase separation and nematic-isotropic-crystal triple point. Inside binodal curves, we find new unstable and metastable regions which are important in phase ordering dynamics. We also find a stable nematic-crystalline (NC) phase, where colloidal particles dispersed in a nematic phase can form a crystalline structure. The coexistence between two NC phases with different concentrations can be appear though the coupling between nematic and crystalline ordering.
Small molecule inhibitors of Aβ‐aggregation and neurotoxicity (2009)

Abstract Alzheimer disease (AD) is characterized pathologically by extracellular amyloid deposits composed of Aβ peptide, neurofibrillary tangles (NFTs) made up of hyperphosphorylated tau, and a deficit of cholinergic neurons in the basal forebrain. Presently, only symptomatic therapies are available for the treatment of AD and these therapies have a limited time frame of utility. Amyloid disorders represent the effects of chronic Aβ production and are not a secondary pathological effect caused by a distant trigger; therefore targeting Aβ is a viable pursuit. In this review, we will discuss the various small molecule anti‐aggregation inhibitors that have been reported in the literature, with emphasis on compounds that are presently being investigated in clinical trials. Drug Dev Res 70, 2009. © 2009 Wiley‐Liss, Inc.
Annotated receipts capture household food purchases from a broad range of sources (2009)

Receipt data provided important quantitative information about HFPB from a wide range of sources and food categories. Two weeks may be adequate to reliably characterize HFPB using annotated receipts.
Ultra high permittivity and significantly enhanced electric field induced strain in PEDOT:PSS–RGO@PU intelligent shape-changing electro-active polymers (2014)

Large deformation of soft materials is harnessed to provide functions in the nascent field of soft machines. In this work, PEDOT:PSS noncovalent functionalized graphene–polyurethane dielectric elastomer composites (PEDOT:PSS–RGO@PU) have been synthesized as promising candidate materials for micro-actuator electromechanical applications. PEDOT:PSS conducting polymer chains not only reinforce the interaction between the polyurethane matrix and graphene sheets, but also prevent graphene sheets from aggregating and connecting, which are beneficial to forming microcapacitors in the matrix and suppressing the leakage current. The PEDOT:PSS–RGO@PU composite exhibits ultra high permittivity (350 at 1 kHz), low dielectric loss (∼0.2 at 1 kHz), low loss modulus (200 MPa), and low loss tangent (∼0.4), all being essential to create a high performance electric-induced strain material. The maximum thickness strain of 164% is significantly higher than reported values for polyurethane elastomers and nanocomposites.
Assembly and budding of a hepatitis B virus is mediated by a novel type of intracellular vesicles (2007)

Our data indicate a strong reorganization of endomembranes during DHBV infection, resulting in the biogenesis of novel organelles serving as multifunctional platforms for assembly and budding of virus progeny.
Micromachined water vapor sensors: a review of sensing technologies (2001)

The measurement of water vapor is important in many applications ranging from predicting changes in the weather to ensuring heating and cooling comfort in homes. In manufacturing, water vapor measurements help to control performance properties of engineered materials and optimize fuel efficiency in power generation. This paper presents a technology review of water vapor sensors and manufacturing techniques. Micromachining, more commonly known as MEMS or microelectromechanical systems, is an enabling technology based upon standard semiconductor manufacturing. MEMS technology makes possible solid-state sensors with greatly reduced power consumption and low operating voltage that are fully compatible with digital electronics and can be manufactured in high volumes at low cost. A water vapor microsensor just becomes another part on the circuit board. A "technology roadmap" for water vapor microsensors is defined where the sensing technologies have been organized into four major transduction schemes: capacitive, mass-sensitive, optical and resistive. Sensing element type, key geometric features and excitation scheme provides further classification. Operating principles and general performance characteristics are also presented.
Neoplastic Conversion of Human Keratinocytes by Adenovirus 12-SV40 Virus and Chemical Carcinogens (1986)

Efforts to investigate the progression of events that lead human cells of epithelial origin to become neoplastic in response to carcinogenic agents have been aided by the development of tissue culture systems for propagation of epithelial cells. In the present study, nontumorigenic human epidermal keratinocytes immortalized by adenovirus 12 and simian virus 40 (Ad 12-SV40) were transformed by treatment with the chemical carcinogens N-methyl-N'-nitro-N-nitrosoguanidine or 4-nitroquinoline-1-oxide. Such transformants showed morphological alterations and induced carcinomas when transplanted into nude mice, whereas primary human epidermal keratinocytes treated with these chemical carcinogens failed to show any evidence of transformation. This in vitro system may be useful in assessing environmental carcinogens for human epithelial cells and in detecting new human oncogenes.
Advances in electrochemical immunosensors (1997)

Abstract The developments in the field of electrochemical immunosensors and immunoassays are reviewed. The enzyme labels suitable for amperometric, potentiometric and conductimetric detection are mentioned. Indirect immunosensors (i.e., that involving enzymatic or electroactive labels) use either heterogeneous or homogeneous formats. Immunoelectrodes are based on conventional electrodes, semiconductor devices are represented by ISFETs and light addressable potentiometric sensors (LAPS). Direct label‐free monitoring of immunoreactions is demonstrated on capacitance systems and pulsed detection using polypyrrole‐modified electrodes. The approaches to regeneration of sensing surfaces are discussed. Examples are given on applications of electrochemical immunodevices in clinical medicine and in environmental monitoring.