gebhart/patrepeval huggingface dataset:
A Novel Grading System Based on Tumor Budding and Cell Nest Size Is a Strong Predictor of Patient Outcome in Esophageal Squamous Cell Carcinoma (2017)

The determination of prognosis in patients with esophageal squamous cell carcinoma (ESCC) is primarily based on staging according to the TNM-classification, whereas conventional grading is of minor clinical importance because of its deficiencies in prognostic patient stratification. Recently, a novel, highly prognostic grading scheme based on budding activity and cell nest size has been proposed for squamous cell carcinoma (SCC) of both pulmonary as well as oral origin. In order to investigate the utility and transferability of this approach to ESCC, we evaluated budding activity and cell nest size, as well as other histomorphologic characteristics, in a cohort of 135 primarily resected tumors and correlated the results with clinicopathologic and outcome parameters. High budding activity and small cell nest size showed a strong association with reduced overall, disease-specific, and disease-free survival (P<0.001, respectively) in ESCC. The combination of both markers in a 3-step grading system showed excellent prognostic separation of well-differentiated (G1), moderately differentiated (G2), and poorly differentiated (G3) carcinomas (P<0.001). The hazard ratio for disease-free survival in multivariate analysis under inclusion of stage was 2.97 for G2 and 5.42 for G3 ESCC (P<0.001). World Health Organization-based grading had no prognostic impact. Taken together, our data prove the value of tumor budding and cell nest size as excellent outcome predictors in ESCC and validate the utility of a previously established grading scheme proposed for oral and pulmonary SCC in this tumor entity. Ultimately, these combined efforts may result in a universal grading system for SCC regardless of the site of origin.
Heterocycles. VIII. Syntheses of 12-acetoxybenzo[c]phenanthridines. (1980)

The compound obtained from the phenacylimide (5) by treatment with p-toluenesulfonic acid and ethylene glycol in benzene is the furoisocarbostyril (7), in contrast to a previous report. Reduction of 5 with sodium borohydride followed by treatment with hydrochloric acid and then hydrogen chloride affords the styrylisocarbostyril (10), photolysis of which provides the benzo [c] phenanthridines (11) and (12), and the amides (13) and (14). The ethylenedioxyimide (8) obtained from 5 affords the styrylisocarbostyrils (18) and (19) by a similar procedure together with enol acetylation. Photolysis of a mixture of 18 and 19 provides the 12-acetoxybenzo [c] phenanthridines (20) and (21).
Human respiratory mucins (1992)

Human respiratory mucins are secreted by goblet cells and mucous glands of the respiratory mucosa. They consist of a broad family of complex glycoproteins with different peptides, or apomucins, corresponding to several genes located on at least three different chromosomes. Glycosylation, the major posttranslational phenomenon, is responsible for about 70-80% of the weight of mucins: it produces an extraordinary diversity of O-glycosidically linked carbohydrate chains which are expressed as several hundreds of different chains in the mucins of a single individual. The variety of mucin peptides and the diversity of carbohydrate chains probably allows many interactions, especially with microorganisms: this may be an essential factor in the defence of the underlying respiratory mucosa.
Diagnosis and Management of Immune Thrombocytopenia in the Era of Thrombopoietin Mimetics (2011)

The recognition of that patients with Immune Thrombocytopenia (ITP) have functional thrombopoietin deficiency and decreased platelet production due to immune-mediated megakaryocytic injury has challenged the traditional view of this disease as predominantly a disorder of antibody-mediated platelet destruction. The therapy of chronic refractory ITP has been transformed by the approval of the thrombopoietin minetics, romiplostim and eltrombopag, which have shown remarkable efficacy in randomized trials. The use of these agents earlier in the disease course after failure of corticosteroid therapy remains controversial. In this article, we review the current data on the efficacy and safety of thrombopoietin receptor agonists and discuss other therapies as well as diagnostic work up of ITP.
Effect of cold development on improvement in electron-beam nanopatterning resolution and line roughness (2006)

This article presents the results about the effect of cold development on the improvement in patterning resolution and line edge roughness for electron-beam lithography resists. Cold development shows improved resolution and contrast for resists that are exposed by polymer chain scission. It does not work for chemically amplified resists. A discussion on why this works for resists like ZEP 520 and PMMA and not for positive chemically amplified resists (such as UV113) is presented. Results for 13 nm structures obtained after metal liftoff using a 30 kV e-beam tool using ZEP 520 resist are shown. These results have impact in the photomask industry and other manufacturers that require squeezing out as much resolution out of their existing tools and materials. It is found that, even with the improvement by cold development, there is a “shot noise” of 2% uncertainty limit that is not surpassed for resists exposed at 100kV. This explains why high throughput and high resolution electron-beam nanolithography is not possible.
Studies on mitochondrial and cytoplasmic malate dehydrogenase in childhood myelodysplastic syndrome (1983)

Three cases of uncommon childhood hematologic disorders are reported. At presentation, one patient had refractory anemia with an excess of blasts (RAEB) with partial 7-monosomy and was reclassified into RAEB “in transformation” thereafter. Another case was diagnosed as acute myelogenous leukemia with complete 7-monosomy. The other case was diagnosed as RAEB “in transformation” without chromosome aberrations. The cytogenetic studies of the patients with 7-monosomy revealed abnormal karyotypes on bone marrow cells, but normal karyotypes on peripheral blood cells. Polymorphonuclear cells from the two patients with 7-monosomy revealed reduced mitochondrial malate dehydrogenase activity, but those from the patient with RAEB “in transformation” without chromosome aberrations did not. Cytoplasmic malate dehydrogenase activity, having been defined as located on chromosome 2, was within the normal range in those three patients. The decreased mitochondrial enzyme activity in the two patients with 7-monosomy would be a dosage effect of the chromosome aberration, but not caused by their hematologic disorders. The level of mitochondrial enzyme activity in the patients with 7-monosomy was reduced in polymorphonuclear cells, but not in mononuclear cells in peripheral blood. This fact would indicate that such chromosome evolution had involved myeloid cells only, but not lymphoid cells. Both enzymes from leukemic cells of four patients with active disease revealed much higher activities than controls, an expression of partially enhanced oxidative phosphorylation.
An efficient two-dimensional blocking strategy for sparse matrix-vector multiplication on GPUs (2014)

Sparse matrix-vector multiplication (SpMV) is one of the key operations in linear algebra. Overcoming thread divergence, load imbalance and non-coalesced and indirect memory access due to sparsity and irregularity are challenges to optimizing SpMV on GPUs.
Indentation responses of a graded zirconium phosphate–filled epoxy resin (2001)

The effects of load and time on the Vickers indentation responses of a graded zirconium phosphate (ZP)–filled epoxy resin are described. The hardness of this material is dependent on the concentration of ZP dispersed within the epoxy matrix. In the region poor in ZP, the hardness response is independent of load. In contrast, the hardness response in the region rich in ZP is profoundly load-dependent as a combined result of particle agglomeration and an indentation-size effect. When compared with the ZP-rich-epoxy, the ZP-poor epoxy exhibits a larger creep and a more pronounced elastic recovery in the Vickers impression. The nature and degree of deformation in the vicinity of Vickers contacts are also studied. During indentation the ZP-rich epoxy exhibits no contact-induced cracks but displays microscale plasticity, which can be associated with intergrain sliding, debonding, and grain push-out. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 931–935, 2001
The Inspiration-Expiration Ratio During Truth and Falsehood. (1921)

EXPER. PSYCH, (to appear shortly
Enhanced parasitization of platelets by plasmodium berghei yoelii (1984)

In previous studies plasmodia have been found by electron microscopy within human platelets naturally infected with Plasmodium vivax and within platelets of mice infected intraperitoneally with P. berghei. In both situations the number of parasitized platelets was low. An enhancement of platelet parasitization was attempted in order to study in greater detail the mechanisms and implications of such a phenomenon. Various in vitro incubation mixtures of normal mouse platelets and free merozoites of the 17X strain of P. berghei yoelii failed to produce any recognizable parasitization of platelets. In vivo, however, large numbers of invaded platelets were obtained by the use of massive intraperitoneal inocula of plasmodia (5 X 10(8) infected erythrocytes). By the 5th day of infection the proportion of parasitized platelets was 13.2 times higher in the animals receiving the large dose than in those receiving the regular passage inoculum (6 X 10(6) infected erythrocytes). Ultrastructural study of 266 intrathrombocytic parasites over eight days of infection failed to show schizogonic maturation beyond the trophozoite state.
DSP based space vector PWM for three-level inverter with DC-link voltage balancing (2002)

The authors describe a novel PWM (pulse width modulation) method for a three-level inverter considering the DC link capacitor balancing problem. Each voltage vector on the space vector plane is classified in relation to the charging/discharging action of DC capacitors, and a novel modulation method is suggested based on the voltage vector selection principle. The algorithm was implemented on the Motorola DSP 56000 and tested with the 7.5 kVA prototype three-level transistor inverter. The effectiveness of the proposed PWM method was verified by the experimental results. It was shown that the proposed PWM techniques are suitable for a high-power GTO (gate turn-off thyristor) three-level inverter satisfying capacitor voltage balancing.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Mapping function to structure in a channel-blocking peptide: electrostatic mutants of charybdotoxin (1992)

Electrostatic interactions between charybdotoxin (CTX), a specific peptide pore blocker of K+ channels, and a Ca(2+)-activated K+ channel were investigated with a genetically manipulable recombinant CTX. Point mutations at certain charged residues showed only small effects on the binding affinity of the toxin molecule: Lys11, Glu12, Arg19, His21, Lys31, and Lys32. Replacement by Gln at Arg25, Lys27, or Lys34 strongly decreased the affinity of the toxin. These affinity changes were mainly due to large increases of toxin dissociation rates without much effect on association rates, as if close-range interactions between the toxin and its receptor site of the channel were disrupted. We also found that the neutralization of Lys27 to Gln removed the toxin's characteristic voltage dependence in dissociation rate. Mutation and functional mapping of charged residues revealed a molecular surface of CTX which makes direct contact with the extracellular mouth of the K+ channel.
Fluoroenzymometric method to measure cardiac troponin I in sera of patients with myocardial infarction (1996)

The aim of our study was to evaluate the clinical relevance of serum troponin I (TnI) as a marker of ischemic myocardial injury by using an automated fluoroenzymometric assay. The reference range for serum TnI was established by measuring serum TnI concentrations in blood from 75 healthy donors. The concentration was then compared with serum creatine kinase (CK) activity, CK-MB mass, and myoglobin concentrations in 20 patients with myocardial infarction diagnosed according to the WHO criteria, 20 patients with chest pain of nonischemic origin, 9 patients with unstable angina, 11 with stable angina, 11 patients with chronic muscular diseases, 6 patients with muscular trauma without chest contusion, and 13 patients with chronic renal disease. We found that: (a) 99% of the blood donors had TnI concentrations <0.26 microgram/L (detection limit of the assay in our study); (b) TnI values in acute myocardial infarction (AMI) patients 4 h after onset of chest pain showed a sensitivity of 0.769 and a specificity of 1.0 at a decisional concentration for AMI of 1 microgram/L, even in the presence of severe skeletal muscle injuries or renal diseases; (c) the increase in TnI concentrations after infarction (interquartile range 3.25-6 h) and the peak occurred later (interquartile range 11.5-24 h) than the rise found in myoglobin and CK-MB, but the increase persisted much longer (>96 h); (d) receiver-operating characteristic curve analysis showed the high diagnostic accuracy of TnI in diagnosing AMI even in patients in whom traditional biochemical markers are adversely influenced by underlying clinical situations.
In search of an osteoblast cell model for in vitro research (2012)

The process of bone formation, remodelling and healing involves a coordinated action of various cell types. Advances in understanding the biology of osteoblast cells during these processes have been enabled through the use of various in vitro culture models from different origins. In an era of intensive bone tissue engineering research, these cell models are more and more often applied due to limited availability of primary human osteoblast cells. While they are a helpful tool in developing novel therapies or biomaterials; concerns arise regarding their phenotypic state and differences in relation to primary human osteoblast cells. In this review we discuss the osteoblastic development of some of the available cell models; such as primary human, rat, mouse, bovine, ovine and rabbit osteoblast cells; as well as MC3T3-E1, MG-63 and SaOs-2 cell lines, together with their advantages and disadvantages. Through this, we provide suggestions on the selection of the appropriate and most relevant osteoblast model for in vitro studies, with specific emphasis on cell-material based studies.
Evidence for Translational Regulation by the Herpes Simplex Virus Virion Host Shutoff Protein (2010)

ABSTRACT The herpes simplex virus (HSV) virion host shutoff protein (vhs) encoded by gene UL41 is an mRNA-specific RNase that triggers accelerated degradation of host and viral mRNAs in infected cells. We report here that vhs is also able to modulate reporter gene expression without greatly altering the levels of the target mRNA in transient-transfection assays conducted in HeLa cells. We monitored the effects of vhs on a panel of bicistronic reporter constructs bearing a variety of internal ribosome entry sites (IRESs) located between two test cistrons. As expected, vhs inhibited the expression of the 5′ cistrons of all of these constructs; however, the response of the 3′ cistron varied with the IRES: expression driven from the wild-type EMCV IRES was strongly suppressed, while expression controlled by a mutant EMCV IRES and the cellular ApaF1, BiP, and DAP5 IRES elements was strongly activated. In addition, several HSV type 1 (HSV-1) 5′ untranslated region (5′ UTR) sequences also served as positive vhs response elements in this assay. IRES activation was also observed in 293 and HepG2 cells, but no such response was observed in Vero cells. Mutational analysis has yet to uncouple the ability of vhs to activate 3′ cistron expression from its shutoff activity. Remarkably, repression of 5′ cistron expression could be observed under conditions where the levels of the reporter RNA were not correspondingly reduced. These data provide strong evidence that vhs can modulate gene expression at the level of translation and that it is able to activate cap-independent translation through specific cis -acting elements.
Pathwise Properties and Performance Bounds for a Perishable Inventory System (2001)

We study a perishable inventory system under a fixed-critical number order policy. By using an appropriate transformation of the state vector, we derive several key sample-path relations. We obtain bounds on the limiting distribution of the number of outdates in a period, and we derive families of upper and lower bounds for the long-run number of outdates per unit time. Analysis of the bounds on the expected number of outdates shows that at least one of the new lower bounds is always greater than or equal to previously published lower bounds, whereas the new upper bounds are sometimes lower than and sometimes higher than the existing upper bounds. In addition, using an expected cost criterion, we compare optimal policies and different choices of critical-number policies.
Recommendations for Achieving Service Levels within Large-scale Resolution Service Networks (2015)

A new recommendation framework that addresses the correct and quick resolution of incidents that occur within the complex systems of an enterprise is introduced here. It uses statistical learning to mediate problem solving by large-scale Resolution Service Networks (with nodes as technical expert groups) that collectively resolve the incidents logged as tickets. Within the enterprise a key challenge is to resolve the tickets arising from operational big data (1) to the customers' satisfaction, and (2) within a time constraint. That is, meet the service level (SL) goals. The challenge in meeting SL is the lack of a global understanding of the types of needed problem solving expertise. Consequently, this often leads to ticket misrouting to experts that are inappropriate for solving the next increment of the problem. The solution here proposes a general two-level classification framework to recommend a SL-efficient sequence of expert groups that jointly can resolve an incoming ticket. The experimental validation shows 34% accuracy improvement over existing locally applied generative models. Additionally, recommended sequences are above 96% likely to meet the enterprise SL goals, which reduces the SL violation rate by 29%. Recommendations are suppressed in the case of non-routine content which is automatically flagged for special attention by humans, since here the humans outperform statistical models.
Low cost guidance for the Multiple Launch Rocket System (MLRS) artillery rocket (2002)

The US Army Aviation and Missile Command has demonstrated the application of advanced technology to significantly improve the accuracy and range of the US Army's Multiple Launch Rocket System (MLRS) through the Guided MLRS Advanced Technology Demonstration (ATD). The addition of a cost-effective guidance and control package to the rocket results in a weapon system that can defeat the target at ranges up to 70 km with significantly fewer rounds. This not only increases the destructive capability of the system but also reduces the cost of the expended ammunition, the cost to transport the ammunition to the combat zone, and the number of launchers required to execute the mission. The guidance kit is housed in the nose of the MLRS and consists of an inertial measurement unit (IMU), four independent electro-mechanically actuated canards, a Global Position System (GPS) receiver, GPS antennas, a thermal battery, a guidance computer, and power supply electronics. Roll decoupling of the warhead and motor section was required to allow roll control of the guidance section to enable accurate inertial navigation and was accomplished by joining the two sections with a roll bearing. Five flight missiles were built and tested during the ATD. Two IMU vendors were selected to provide flight units (3 flights used vendor A and 2 flights used vendor B). A tightly coupled 8 channel GPS receiver was flown on all flights. This paper discusses the ATD development effort and presents flight test results.
Regulation of cell pH by Ca+2-mediated exocytotic insertion of H+-ATPases. (1986)

Exposure to CO2 acidifies the cytosol of mitochondria-rich cells in turtle bladder epithelium. The result of the decrease in pH in these, the acid-secreting cells of the epithelium, is a transient increase in cell calcium, which causes exocytosis of vesicles containing proton-translocating ATPase. Because mitochondria-rich cells have rapid luminal membrane turnover, we were able to identify single mitochondria-rich cells by their endocytosis of rhodamine-tagged albumin. Using fluorescence emission of 5,6-carboxyfluorescein at two excitation wavelengths, we measured cell pH in these identified mitochondria-rich cells and found that although the cell pH fell, it recovered within 5 min despite continuous exposure to CO2. This pH recovery also occurred at the same rate in Na+-free media. However, pH recovery did not occur when luminal pH was 5.5, a condition under which the H+-pump does not function, suggesting that recovery of cell pH is due to the luminally located H+ ATPase. Chelation of extracellular calcium by EGTA prevented the CO2-induced rise in cell calcium measured with the intracellular fluorescent dyes Quin 2 or Fura 2 and also prevented recovery of cell pH. When the change in cell calcium was buffered by loading the cells with high concentrations of Quin 2, the CO2-induced decrease in pH did not return back to basal levels. We had found previously that buffering intracellular calcium transients prevented CO2-stimulated exocytosis. Further, we show here that the increased H+ current in voltage-clamped turtle bladders, which is directly proportional to the number of H+-pump-containing vesicles that fuse with the luminal membrane, was significantly reduced in calcium-depleted bladders. These results suggest that pH regulation in these acid-secreting cells occurs by calcium-dependent exocytosis of vesicles containing proton pumps, whose subsequent turnover restores the cell pH to its initial levels.
Prostate Specific Antigen and Local Recurrence after Radical Prostatectomy (1990)

We evaluated the location of recurrent disease in 63 patients with carcinoma of the prostate who had abnormal levels of prostate specific antigen (greater than 0.4 ng./ml., Tandem-R assay) 6 to 240 months after radical prostatectomy but who were otherwise considered to be without evidence of disease. The evaluation involved physical examination including digital rectal examinations by 3 urologists, isotopic bone scans, computerized tomography scans of the abdomen and pelvis, cystoscopy, and random needle biopsies of the urethrovesical anastomotic area. In 6 patients metastatic disease to the bone and/or lymph nodes was found and local prostate cancer was discovered in 5. Among 57 patients without evidence of disease by the usual methods of evaluation needle biopsies of the anastomosis revealed local disease in 42%. No local disease was discovered in 30 post-radical prostatectomy patients with normal prostate specific antigen levels. There was a wide range of transrectally palpable contours after radical prostatectomy in patients with and without elevated prostate specific antigen levels. We conclude that prostate specific antigen but not digital rectal examination is an excellent early indicator of possible local recurrence after radical prostatectomy. Whether the prevalence of local disease after radical prostatectomy using prostate specific antigen levels and needle biopsies of the anastomosis is greater than heretofore has been appreciated will require further study.
Blood DNA methylation and COVID-19 outcomes (2021)

Our data contribute to the awareness that DNA methylation may influence the expression of genes that regulate COVID-19 progression and represent a targetable process in that setting.