gebhart/patrepeval huggingface dataset:
Living Radical Polymerization with Alkali and Alkaline Earth Metal Iodides as Catalysts (2016)

The generation of an alkyl radical (R•) from an alkyl iodide (R–I) with NaI playing a catalytic role was experimentally demonstrated. This catalytic reaction was exploited as an activation process for living radical polymerization. Alkali metal iodides, NaI, KI, and CsI, and alkaline earth metal iodides, MgI2 and CaI2, were systematically studied as catalysts. 18-crown-6-Ether and a polyether, that is, diethylene glycol dimethyl ether (diglyme), were utilized to solvate these catalysts in hydrophobic monomers. Among the five catalysts, NaI exhibited a particularly high reactivity. The polymer molecular weight and its distribution (Mw/Mn = 1.2−1.4) were well controlled with high conversions (e.g., 80–90%) in reasonably short periods of time (3–6 h) at mild temperatures (60–70 °C) in the polymerizations of methyl methacrylate. NaI is also amenable to styrene, acrylonitrile, and functional methacrylates. In addition to homopolymers, NaI also afforded well-defined block copolymers, chain-end functional polymers, and a star polymer. The high monomer versatility and accessibility to a wide range of polymer architectural designs are desirable features of this polymerization system.
Gibberellin Signaling (2002)

The power of molecular genetics has dramatically advanced our understanding of all aspects of gibberellin (GA) signaling. Many genes encoding GA response pathway components have been identified using Arabidopsis and cereal mutants, and more elaborate genetic screens are producing additional mutants
Added Value of Dialogic Parent–Child Book Readings: A Meta-Analysis (2008)

Book reading has been demonstrated to promote vocabulary. The current study was conducted to examine the added value of an interactive shared book reading format that emphasizes active as opposed to noninteractive participation by the child. Studies that included a dialogic reading intervention group and a reading-as-usual control group, and that reported vocabulary as an outcome measure were located. After extracting relevant data from 16 eligible studies, a meta-analysis was conducted to attain an overall mean effect size reflecting the success of dialogic reading in increasing children's vocabulary compared to typical shared reading. When focusing on measures of expressive vocabulary in particular (k = 9, n = 322), Cohen's d was .59 (SE = .08; 95% CI = 0.44, 0.75; p < .001), which is a moderate effect size. However, the effect size reduced substantially when children were older (4 to 5 years old) or when they were at risk for language and literacy impairments. Dialogic reading can change the home literacy activities of families with 2- to 3-year-old children but not those of families with children at greatest risk for school failure.
Calcium/phospholipid-dependent protein kinase (protein kinase C) phosphorylates and activates tyrosine hydroxylase. (1984)

Protein kinase C, purified to homogeneity, was found to phosphorylate and activate tyrosine hydroxylase that had been partially purified from pheochromocytoma PC 12 cells. These actions of protein kinase C required the presence of calcium and phospholipid. This phosphorylation of tyrosine hydroxylase reduced the Km for the cofactor 6-methyltetrahydropterine from 0.45 mM to 0.11 mM, increased the Ki for dopamine from 4.2 microM to 47.5 microM, and produced no change in the Km for tyrosine. Little or no change in apparent Vmax was observed. These kinetic changes are similar to those seen upon activation of tyrosine hydroxylase by cAMP-dependent protein kinase. Two-dimensional phosphopeptide maps of tyrosine hydroxylase were identical whether the phosphorylation was catalyzed by protein kinase C or by the catalytic subunit of cAMP-dependent protein kinase. Both protein kinases phosphorylated serine residues. The results suggest that protein kinase C and cAMP-dependent protein kinase phosphorylate the same site(s) on tyrosine hydroxylase and activate tyrosine hydroxylase by the same mechanism.
PPARγ and PGC-1α as Therapeutic Targets in Parkinson’s (2014)

The peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-activated transcriptional factor that belongs to the nuclear hormone receptor superfamily. PPARγ was initially identified through its role in the regulation of glucose and lipid metabolism and cell differentiation. It also influences the expression or activity of a number of genes in a variety of signalling networks. These include regulation of redox balance, fatty acid oxidation, immune responses and mitochondrial function. Recent studies suggest that the PPARγ agonists may serve as good candidates for the treatment of several neurodegenerative disorders including Parkinson's disease (PD), Alzheimer's disease, Huntington's disease and amyotrophic lateral sclerosis, even though multiple etiological factors contribute to the development of these disorders. Recent reports have also signposted a role for PPARγ coactivator-1α (PGC-1α) in several neurodegenerative disorders including PD. In this review, we explore the current knowledge of mechanisms underlying the beneficial effects of PPARγ agonists and PGC-1α in models of PD.
A key-chain-based keying scheme for many-to-many secure group communication (2004)

We propose a novel secure group keying scheme using hash chain for many-to-many secure group communication. This scheme requires a key predistribution center to generate multiple hash chains and allocates exactly one hash value from each chain to a group member. A group member can use its allocated hash values (secrets) to generate group and subgroup keys. Key distribution can be offline or online via the key distribution protocol. Once keys are distributed, this scheme enables a group member to communicate with any possible subgroups without the help of the key distribution center, and without having to leave the overall group, thus avoiding any setup delay. Our scheme is suitable for applications where the population of a system is stable, group size is moderate, subgroup formation is frequent, and the application is delay sensitive. Through analysis, we present effectiveness of our approach.
The Blackwell Guide to the Philosophy of Computing and Information (2003)

Notes on Contributors. Preface Part I: Four Concepts:1. B. Jack Copeland, Computation.2. Alasdair Urquhart, Complexity.3. Klaus Mainzer, System: An Introduction to Systems Science.4. Luciano Floridi, Information.Part II: Computers in Society:5. Deborah G Johnson, Computer Ethics.6. Charles Ess, Computer-mediated Communication and Human - Computer Interaction.7. Wesley Cooper, Internet Culture.8. Dominic McIver Lopes, Digital Art.Part III: Mind and AI:9. James H.Fetzer, The Philosophy of AI and its Critique.10. Brian P. McLaughlin, Computationalism, Connectionism and the Philosophy of Mind.Part IV: Real and Virtual Worlds:11. Barry Smith, Ontology.12. Derek Stanovsky, Virtual Reality.13. Eric Steinhart, The Physics of Information.14. Roberto Cordeschi, Cybernetics.15. Mark A. Bedau, Artificial Life.Part V: Language and Knowledge:16. Jonathan Cohen, Information and Content.17. Fred Adams, Knowledge.18. Graham White, The Philosophy of Computer Languages.19. Thierry Bardini, Hypertext.Part VI: Logic and Probability:20. G. Aldo Antonelli, Logic.21. Donald Gillies, Probability in Artificial Intelligence.22. Cristina Bicchieri, Game Theory: Nash Equilibrium.Part VII: Science and Technology:23. Paul Thagard, Computing in the Philosophy of Science.24. Timothy Colburn, Methodology of Computer Science.25. Carl Mitcham, Philosophy of Information Technology.26. Patrick Grim, Computational Modeling as a Philosophical Methodology.Index.
Dyspnea and Symptom Amplification in Asthma (2007)

A physician may make a reasonable estimate of an asthmatic patient's lung function from the intensity of his complaint only if he - the physician - considers the patient's tendency to symptom amplify as well.
Differential effects of transforming growth factor-beta on proliferation of normal and malignant rat liver epithelial cells in culture. (1986)

Transforming growth factors (TGF-betas) have been shown to cause both stimulatory and inhibitory effects on cellular growth in a variety of normal and neoplastic cells. The nature of the inhibitory effects of TGF-beta on proliferation of different cell types is at present unclear. We have used freshly isolated rat hepatocytes, a normal diploid rat liver epithelial cell line (NRLM), and a subline (AFB) derived from it which was transformed in vitro by aflatoxin B1 to study the nature of TGF-beta-induced growth inhibition and its alteration following chemically induced neoplastic transformation. TGF-beta had a vastly different effect on proliferation of normal rat liver epithelial cells (both freshly isolated and NRLM cells) compared to aflatoxin B1-transformed cells. TGF-beta at 20 pg/ml caused 83% inhibition of colony formation of NRLM, whereas the growth of AFB cells was unaffected by TGF-beta at concentrations as high as 10 ng/ml. A parallel dose-dependent inhibition of DNA synthesis by TGF-beta was observed in both primary hepatocytes and NRLM cells at concentrations between 10 pg and 10 ng/ml. No inhibition of DNA synthesis was observed in AFB cells. Furthermore, TGF-beta did neither induce anchorage-independent growth of NRLM cells nor affect the growth of AFB cells in soft agar. TGF-beta-induced inhibition of the NRLM cells was irreversible in nature, since treated cells were unable to proliferate and form colonies upon removal of TGF-beta from the medium. Also, NRLM cells showed, after 4 days in the presence of 20 pg of TGF-beta per ml morphological changes characterized by cytoplasmic hypertrophy and the formation of abundant liposomal derivatives, some of which resemble lipofuscin. The finding that TGF-beta caused a high degree of irreversible inhibition of NRLM cells emphasizes the need for caution in interpreting data from inhibition studies, since most assays presently used are designed for assessing growth stimulation in vitro and do not adequately distinguish between the possible cytotoxic and/or cytostatic action of growth inhibitors.
Review of Chitin and Chitosan as Fiber and Film Formers (1994)

Abstract Chitin is one of the most abundant polysaccharides found in nature. It is often considered a cellulose derivative although it does not occur in organisms producing cellulose. Cellulose consists of β-(1→4)-D-glucopyranose units. In contrast, chitin has the same backbone but the 2-hydroxy has been replaced by an acetamide group, resulting in mainly β-(1→4)-2-acetamido-2-deoxy-D- glucopyranose structural units (GLcNAc). Chitosan is the N-deacetylated derivative of chitin, though this N-deacetylation is almost never complete. A sharp nomenclature border has not been defined between chitin and chitosan based on the degree of N-acetylation [1, 2]. Structural examples of cellulose, chitin, and chitosan can be found in Fig. 1. The structural and chemical features will be elaborated on in a later section.
Rescue of sarcoglycan mutations by inhibition of endoplasmic reticulum quality control is associated with minimal structural modifications (2011)

Sarcoglycanopathies (SGP) are a group of autosomal recessive muscle disorders caused by primary mutations in one of the four sarcoglycan genes. The sarcoglycans (α-, β-, γ-, and δ-sarcoglycan) form a tetrameric complex at the muscle membrane that is part of the dystrophin-glycoprotein complex and plays an essential role for membrane integrity during muscle contractions. We previously showed that the most frequent missense mutation in α-sarcoglycan (p.R77C) leads to the absence of the protein at the cell membrane due to its blockade by the endoplasmic reticulum (ER) quality control. Moreover, we demonstrated that inhibition of the ER α-mannosidase I activity using kifunensine could rescue the mutant protein localization at the cell membrane. Here, we investigate 25 additional disease-causing missense mutations in the sarcoglycan genes with respect to intracellular fate and localization rescue of the mutated proteins by kifunensine. Our studies demonstrate that, similarly to p.R77C, 22 of 25 of the selected mutations lead to defective intracellular trafficking of the SGs proteins. Six of these were saved from ER retention upon kifunensine treatment. The trafficking of SGs mutants rescued by kifunensine was associated with mutations that have moderate structural impact on the protein.
Studies on the Mutation of Aspergillus niger (1982)

Several mutants were isolated from the parent strain of Aspergillus niger CF: the first mutant strain CF-11 was obtained by UV irradiation, and the second mutant strain CF-21 and CF-22 were from NTG (N-methyl-N'-nitroso-N-nitroso-guanidine) treatment on the CF-11. These mutants were characterized, and their enzyme and acid production on wheat bran Koji and wheat flour Koji were studied. Asp. niger CF-22 mutant appeared to be tan type which conidial heads were discolored. It's glucoamylase activity was inreased approximately two times and its about 50 percent as compared with that of the parent strain of Asp. niger CF, when grown on wheat bran Koji under the optimal conditions. Asp. niger CF-21 mutant showed slower growth rate and poor sporulation than the wild type, although its conidial heads were not discolored. Approximately 4-fold increment in its acid production was observed as compared with the weld type. The activities of glucoamylase and of the Asp. niger CF-22 and CF-21 mutants were higher than those of the wild type, but their protease activity was rather lower. The maximum production of glucoamylase by the Asp. niger CF-22 mutant was obtained after 2 to 3 days incubation on wheat bran at 30 to 2 days incubation at 30 to . The maximal levels of acid production by the mutant CF-21 was appeared after 2 days incubation on wheat bran Koji, and after 3 days on wheat flour Koji at . Little differences in the levels of acid production were observed between on wheat bran and flour Koji.
Zeta Potential of Extracellular Vesicles: Toward Understanding the Attributes that Determine Colloidal Stability (2020)

Extracellular vesicles (EVs), including exosomes and microvesicles (<200 nm), play a vital role in intercellular communication and carry a net negative surface charge under physiological conditions. Zeta potential (ZP) is a popular method to measure the surface potential of EVs, while used as an indicator of surface charge, and colloidal stability influenced by surface chemistry, bioconjugation, and the theoretical model applied. Here, we investigated the effects of such factors on ZP of well-characterized EVs derived from the human choriocarcinoma JAr cells. The EVs were suspended in phosphate-buffered saline (PBS) of various phosphate ionic concentrations (0.01, 0.1, and 1 mM), with or without detergent (Tween-20), or in the presence (10 mM) of different salts (NaCl, KCl, CaCl<sub>2</sub>, and AlCl<sub>3</sub>) and at different pH values (4, 7, and 10) while the ZP was measured. The ZP changed inversely with the buffer concentration, while Tween-20 caused a significant (<i>p</i> < 0.05) lowering of the ZP. Moreover, the ZP was significantly (<i>p</i> < 0.05) less negative in the presence of ions with higher valency (Al<sup>3+</sup>/Ca<sup>2+</sup>) than in the presence of monovalent ones (Na<sup>+</sup>/K<sup>+</sup>). Besides, the ZP of EVs became less negative at acidic pH, and <i>vice versa</i>. The integrated data underpins the crucial role of physicochemical attributes that influence the colloidal stability of EVs.
Running and cementing deep well liners (1975)

Relatively little attention is given to operations performed on a well after a liner has been set and cemented. However, post cementing procedures can be critically important to the success of a liner setting job and merit careful consideration. This discussion recommends some specific post cementing practices which have been used successfully on ultradeep liner jobs and are considered field proven. Thus, it is important that the recommended production liner cleanout procedures be closely followed, since they usually are performed under low clearance conditions with lower strength work strings and with a higher incidence of fishing jobs. Included are descriptions of equipment and techniques used for installing stub liners as well as full liner tieback strings.
A Practical Synthesis of Cabergoline (2002)

Cabergoline is an N-acylurea derived from 9,10-dihydrolysergic acid, which is a potent prolactin inhibitor. It is marketed by Pharmacia as Dostinex for the treatment of hyperprolactinemia and is currently under active development for the treatment of a variety of CNS disorders. In the existing process, the N-acylurea is formed by the reaction of an amide with a large excess of ethyl isocyanate at elevated temperatures. An improved process was developed that eliminates this hazardous reaction. The amide is reacted with phenyl chloroformate and then with ethylamine, which provides a mild and efficient means of forming the unsymmetrical N-acylurea.
Tuning Nanoparticle Uptake: Live-Cell Imaging Reveals Two Distinct Endocytosis Mechanisms Mediated by Natural and Artificial EGFR Targeting Ligand (2012)

Therapeutic nanoparticles can be directed to cancer cells by incorporating selective targeting ligands. Here, we investigate the epidermal growth factor receptor (EGFR)-mediated endocytosis of gene carriers (polyplexes) either targeted with natural EGF or GE11, a short synthetic EGFR-binding peptide. Highly sensitive live-cell fluorescence microcopy with single particle resolution unraveled the existence of two different uptake mechanisms; EGF triggers accelerated nanoparticle endocytosis due to its dual active role in receptor binding and signaling activation. For GE11, an alternative EGFR signaling independent, actin-driven pathway is presented.
Chronological aspects of ultrasonic, hormonal, and other indirect indices of ovulation (2001)

For accuracy and practical reasons, the cervical mucus peak symptom, the ratio of urinary metabolites and luteinising hormone initial rise might be better indices of ovulation than the luteinising hormone peak.
Competence‐specific induction of <i>recA</i> is required for full recombination proficiency during transformation in <i>Streptococcus pneumoniae</i> (1998)

Transcriptional activation of the recA gene of Streptococcus pneumoniae was previously shown to occur at competence. A 5.7 kb recA-specific transcript that contained at least two additional genes, cinA and dinF, was identified. We now report the complete characterization of the recA operon and investigation of the role of the competence-specific induction of recA. The 5.7 kb competence-specific recA transcript is shown to include lytA, which encodes the pneumococcal autolysin, a protein previously shown to contribute to virulence of S. pneumoniae. Uncoupling (denoted Ind-) of recA and/or the downstream genes was achieved through the placement of transcription terminators within the operon, either upstream or downstream of recA. Prevention of the competence-specific induction of recA severely affected spontaneous transformation. Transformation efficiencies of recA+ (Ind-) and of wild-type cells were compared under various conditions and with different donor DNA. Chromosomal transformation was reduced 17-(chromosomal donor) to 45-fold (recombinant plasmid donor), depending on the donor DNA, and plasmid establishment was reduced 129-fold. Measurement of uptake of radioactively labelled donor DNA in transformed cells in parallel with scoring for transformants (chromosomal donor) revealed normal uptake, but a 21-fold reduction in recombination in a recA+ (Ind-) strain, indicating that the transformation defect was primarily in recombination. Strikingly enough, a much larger (460-fold) reduction in recombination was observed for the shortest homologous donor fragment used (878 nucleotides long). Possible interpretations of the observation that basal RecA appears unable to promote efficient recombination whatever the number and the length of donor fragments taken up are proposed. The role of recA induction is discussed in view of the potential contribution of transformation to genome plasticity in this pathogen.
Interface state energy distribution and Pb defects at Si(110)/SiO2 interfaces: Comparison to (111) and (100) silicon orientations (2011)

Traps at the (110)Si/SiO2 interface are investigated by combining electrical methods with electron spin resonance (ESR) measurements, and the results are compared to the well studied (100) and (111)Si/SiO2 interfaces. At all three Si crystal faces, the interface trap density Dit as function of energy E in the Si band gap exhibits two peaks at about 0.25 and 0.85 eV above the Si valence band, found to be well correlated with Pb(0) centers (Si3≡Si• defects). By comparing capacitance-voltage (CV) curves at 300 and 77 K of both n- and p-type samples, the Pb(0) defects are confirmed to be amphoteric. Effective passivation of interface traps by H2 annealing suggests that Pb0 defects are responsible for most of interface traps observed in (110)Si/SiO2. The truly amphoteric behavior, implying that one Pb0 defect delivers two interface trap levels, was observed for the (100) and (111)Si faces but not for the (110) face. The estimated interface trap density Nit at the (110)Si/SiO2 interface oxidized at 930 °C is (6.7±0.5)×1012, while the Pb0 density as determined by ESR is about (6±1)×1012 cm−2. Lowering of the oxidation temperature leads to further reduction in the electrically active Pb0 centers fraction at the (110)Si/SiO2 interface.
A study of degradation of high density polyethylene in a corotating intermeshing twin screw extruder (1993)

Abstract A study of the effect of extrusion conditions on the degradation of high density polyethylene was carried out. The extent of degradation was quantified by dynamic viscometry. The effect of barrel temperature profile, screw speed, and feed rate on the degradation of high density polyethylene was determined. The viscosity response of high density polyethylene is complex with respect to extrusion conditions. Residence time studies were carried out to correlate the viscosity data. Simulations of simple theoretical models give an insight into the flow behavior of the polymer in a twin screw extruder.
Using Chemopreventive Agents to Enhance the Efficacy of Cancer Therapy (2006)

Emerging evidence suggests that cancer preventative agents might be combined with chemotherapy or radiotherapy for the more effective treatment of cancer. Recent studies suggest that genistein and other dietary compounds that prevent cancer may enhance the efficacy of cancer therapeutics by modifying the activity of key cell proliferation and survival pathways, such as those controlled by Akt, nuclear factor-kappaB, and cyclooxygenase-2. In this article, we summarize the findings of recent investigations of chemopreventive agents in combination with cancer treatment regimens.