gebhart/patrepeval huggingface dataset:
Effects of Repeated Exhaustive Exercise on Myocardial Subcellular Membrane Structures (1988)

The effects of a single vs repeated bouts of exhaustive exercise on myocardial integrity were evaluated in the rodent. Rats were randomly divided into three groups: a sedentary control, single swim-to-exhaustion, and seven consecutive daily swims-to-exhaustion groups (n = 5 in each group). Overall morphology was evaluated histologically and sarcolemmal (SL), sarcotubular (ST), and mitochondrial (MITO) membranes were examined ultrastructurally using transmission electron microscopy. Neither single nor repeated bouts of exhaustive exercise produced myocardial hypoxia, as assessed by MITO morphology. However, increased interstitial space as well as significant swelling (P less than 0.01) was observed in ST from both 1- and 7-day exhausted animals. These findings were not observed at all in control myocardium. Higher magnification (x 45,000) in some instances revealed whole disruption of sarcotubular membranes in myocardium from swim-exhausted animals. The incidence of membrane disruption was higher (P less than 0.01) in 7-day vs 1-day swim-exhausted groups and not observed at all in control rats. Although in no instance did we observe disruption of SL membranes, their convoluted nature in myocardium from both exercised groups indicated exhaustion-induced fluid and/or ionic shifts within the left ventricle. These findings provide a structural basis for the reduction in myocardial sarcoplasmic reticulum and MITO Ca++ uptake previously observed following repeated bouts of exhaustive exercise.
CT-guided radiofrequency ablation of a bronchogenic carcinoma (2003)

We report on the successful use of percutaneous CT-guided radiofrequency ablation (RFA) of a peripheral bronchogenic carcinoma in a 73-year-old patient. RFA was the favoured treatment option in this patient, who owing to comorbid factors was not a candidate for surgery. A 15 G LeVeen Needle Electrode (RadioTherapeutics, Sunnyvale, USA) with an array diameter of 3.0 cm was connected to a 200 Watt Generator (RF 3000, RadioTherapeutics, Sunnyvale, USA) and inserted into a 3.5 cm squamous cell carcinoma of the axillary subsegment of the right upper lobe. RFA resulted in complete tumour necrosis confirmed by histopathological examination. No complications such as a pneumothorax or bleeding occurred. Further clinical experience and prospective studies are necessary to determine the long-term efficacy of RFA in the treatment of lung tumours.
Development and Bench Validation of Real-Time Reverse Transcription Polymerase Chain Reaction Protocols for Rapid Detection of the Subtypes H6, H9, and H11 of Avian Influenza Viruses in Experimental Samples (2007)

Real-time reverse transcription polymerase chain reaction (RRT-PCR) is commonly used for the rapid detection, as well as to determine the subtype, of avian influenza viruses (AIVs). There are 16 known serologically distinct hemagglutinin (HA) subtypes of AIV described. Currently, determination of the subtypes of AIVs by RRT-PCR tests has been limited to the H5 and H7 subtypes. In this study, RRT-PCR assays were developed in simplex formats for rapid detection of AIV subtypes H6, H9, and H11. The primers and probes for RRT-PCR were designed from nucleotide sequences of the HA genes, which were either downloaded from GenBank (for H6 and H9) or sequenced for this study. The specificity and sensitivity of the RRT-PCR assays were determined based on the detection of the virus from a proficiency panel consisting of 15 different HA subtypes of AIVs and from serial dilutions of target viral RNA. The subtype-specific RRT-PCR assays were used to detect the virus in cloacal and oropharyngeal swabs of experimental chickens inoculated with H6, H9, and H11 AIVs, and the test results were compared with validated RRT-PCR assays based on the amplification of AI matrix (MA) gene. A high correlation of the matrix test and the specific H6, H9, and H11 by the RRT-PCR assays was observed; kappa coefficients for the agreement of test results in cloacal and oropharyngeal swabs combined were 0.927, 0.962, and 0.981, respectively.
Fast object detection for robots in a cluttered indoor environment using integral 3D feature table (2011)

Realizing automatic object search by robots in an indoor environment is one of the most important and challenging topics in mobile robot research. If the target object does not exist in a nearby area, the obvious strategy is to go to the area in which it was last observed. We have developed a robot system that collects 3D-scene data in an indoor environment during automatic routine crawling, and also detects objects quickly through a global search of the collected 3D-scene data. The 3D-scene data can be obtained automatically by transforming color images and range images into a set of color voxel data using self-location information. To detect an object, the system moves the bounding box of the target object by a certain step in the color voxel data, extracts 3D features in each box region, and computes the similarity between these features and the target object's features, using an appropriate feature projection learned beforehand. Taking advantage of the additive property of our 3D features, both feature extraction and similarity calculation are considerably accelerated. In the object learning process, the system obtains the feature-projection matrix by weighting unique features of the target object rather than its common features, resulting in reducing object detection errors.
Linear phase detection using two-phase latch (2003)

Modified two-phase latch and flip-flop are introduced to implement a linear phase-detector (LPD) for 1/N-rate clock recovery applications. This technique greatly simplifies the required circuitry of the LPD and makes it suitable for higher speed applications while consuming less power compared to the conventional techniques.
Clinical evaluation of NCA in patients with chronic myelocytic leukemia (1982)

NCA, a normal colon and granulocyte antigen, which has been found in large amounts in myelocytes and metamyelocytes and in smaller amounts in neutrophil granulocytes, was studied in 50 CML patients in various stages of the disease. Radioimmunoassay was used to demonstrate NCA in serum. Untreated CML patients had a mean level of 732 micrograms NCA/l, poorly controlled patients 421 micrograms/l and well-controlled patients 160 mu/l. These values differ significantly from the mean of healthy persons, which was 71 micrograms NCA/l. The serum NCA levels were related to the number of maturing myeloid cells in blood, and to the clinical course in the chronic phase of CML. In blast crisis low values with a mean of 109 micrograms NCA/l was found. Patients with ANLL had a low mean level, 50 micrograms/l. Low levels of NCA could not be attributed to antibodies to NCA. NCA is a normal myeloid differentiation antigen. Despite this, its occurrence in serum in leukemic patients differs from normal. This probably has to do with the abnormal amount as well as the release of NCA by leukemic maturing myeloid cells.
The Histochemical Localization of Several Enzymes of Soybeans Infected with the Root-knot Nematode Meloidogyne incognita acrita. (1969)

The sites of activity of alkaline phosphatase, acid phosphatase, esterase, peroxidase, adenosine triphosphatase, and cytochrome oxidase were demonstrated histochemically in fresh sections of 'Lee' soybeans infected by the root-knot nematode Meloidogyne incognita acrita. Each of the six enzymes was more active at the sites of infection than in adjacent non-infected tissue. During the early stages of infection, an increase in enzyme activity was observed in several cells in the proximity of the lip region of the nematode. However, when definite syncytia were observed, increased enzyme activity was confined primarily within the limits of the syncytium. Increased activity paralleled syncytial development and nematode maturation.
Expression and occupancy of BAFF‐R on B cells in systemic lupus erythematosus (2005)

BAFF-R is consistently occupied on blood B cells in SLE. Occupancy of BAFF-R on blood B cells is likely to contribute to disease mechanisms in SLE and could serve as a biomarker of disease activity. Targeting BLyS as a therapeutic strategy will require overcoming the persistent binding of BLyS to BAFF-R.
<sup>13</sup>C-Pattern of Natural Glycerol:  Origin and Practical Importance (1997)

The average δ13C-value of glycerol from plant origin is 4−5‰ more negative than that of carbohydrates from the same source. This depletion is exclusively dealing with position C-1 of the molecule. This is also observed for glycerol from other natural sources, although to a smaller extent, while synthetic glycerol shows a statistical 13C-pattern. On the basis of these data an illegal addition of glycerol to wine should be detectable. In fermentations of glucose with yeast the extent of the depletion in position C-1 of glycerol proved to be reciprocal to its yield. Furthermore a total carbon and isotope balance showed that the depletion of glycerol is a compensation for small 13C-enrichments in the corresponding positions of main products. An isotope effect on the aldolase reaction and a noncomplete equilibration of the triose phosphates are discussed as main reasons for these findings. Keywords: Authenticity proof; carbon-13; glycerol; isotopic pattern; stable isotopes; wine
Sleep Patterns and Mortality among Elderly Patients in a Geriatric Hospital (2000)

Sleep disturbance may be one of the symptoms indicating poor health or functional deficits, and be an independent risk factor for survival.
Polar Assembly of <i>N</i>,<i>N</i>‘-Bis(4-substituted benzyl)sulfamides (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTPolar Assembly of N,N'-Bis(4-substituted benzyl)sulfamidesBing Gong, Chong Zheng, Huaqiang Zeng, and Jin ZhuView Author Information Department of Chemistry, The University of Toledo Toledo, Ohio 43606 Department of Chemistry and Biochemistry Northern Illinois University, DeKalb, Illinois 60115 Cite this: J. Am. Chem. Soc. 1999, 121, 41, 9766–9767Publication Date (Web):October 4, 1999Publication History Received12 July 1999Published online4 October 1999Published inissue 1 October 1999https://pubs.acs.org/doi/10.1021/ja992432jhttps://doi.org/10.1021/ja992432jrapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views411Altmetric-Citations39LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Crystal structure,Crystals,Layers,Molecules,Space group Get e-Alerts
Morphometric assessment of mature and diminished-maturity human spermatozoa: sperm regions that reflect differences in maturity* (1999)

As part of our studies on sperm maturity and function, we examined the head, midpiece and tail of human spermatozoa using computerized morphometry in order to determine which regions reflect the differences between mature spermatozoa and spermatozoa of diminished cellular maturity. We studied 20 men, who were divided into two groups based on their lower (LCKM: 14.6 +/- 7.0%, n = 8) and higher sperm creatine kinase (CK-M) isoform ratios (HCKM: 48.0 +/- 4.3%, n = 12) in the initial semen. Using a sequential centrifugation method which relies on the lower density of immature spermatozoa with retained extra cytoplasm, we prepared three sperm fractions with progressively declining maturity, as confirmed with CK-M isoform ratio measurements. Following the sequential fractionation, we affixed the spermatozoa to glass slides, stained the midpiece and the sperm contour, and photographed 25 spermatozoa in each of the 60 fractions (1509 spermatozoa in all). The spermatozoa were then individually digitized on the Image-1 system, and the dimensions of the head, midpiece, and tail were determined. While the data showed significant differences in the midpiece and tail dimensions between the mature and diminished-maturity sperm fractions, the head dimensions were similar and did not reflect sperm maturity. We postulated that the relationship between the biochemical markers of sperm maturity and sperm morphology is based on common spermiogenic events. The data support this idea. In immature spermatozoa in which cytoplasmic extrusion, CK-M isoform expression, and tail sprouting are all diminished, the retained extra cytoplasm in the midpiece and shorter tail length contribute to the morphological variations that we identified by morphometry and considered in sperm morphology. These morphometric features, in association with fluorochrome-coupled biochemical probes, can facilitate the identification of mature spermatozoa in computer-assisted semen analysis.
Native Recombinant Cyclophilins A, B, and C Degrade DNA Independently of Peptidylprolyl cis-trans-Isomerase Activity (1997)

Previous work in our laboratory (Montague, J., Gaido, M., Frye, C., and Cidlowski, J. (1994) J. Biol. Chem. 269, 18877-18880) has shown that human recombinant cyclophilins A, B, and C have sequence homology with the apoptotic nuclease NUC18 and that denatured cyclophilins can degrade DNA. We have now evaluated the nucleolytic activity of recombinant cyclophilins under native conditions. We show that nuclease activity inherent to cyclophilins is distinct from cis-trans-peptidylprolyl isomerase activity and is similar to that described for apoptotic nucleases. Cyclophilin nucleolytic activity is stimulated by Ca2+ and/or Mg2+, with a combination of the two being optimal for cyclophilins A and B. Mg2+ alone is sufficient for cyclophilin C nuclease activity. pH optimums are in the range of pH 7.5-9.5. Cyclophilins can degrade both single-stranded and double-stranded DNA. Additionally, cyclophilins produce 3'-OH termini in linear double-stranded substrates, suggesting the cuts produced are similar to those of apoptotic cells. Cyclophilins also display endonucleolytic activity, demonstrated by their ability to degrade supercoiled DNA. In the absence of ions, cyclophilins bind linearized DNA. When added to nuclei from nonapoptotic cells, cyclophilin C induces 50-kilobase pair DNA fragmentation but not internucleosomal fragmentation. Together, these data suggest that cyclophilins are involved in degradation of the genome during apoptosis.
A Frequency- and Pattern-Reconfigurable Center-Shorted Microstrip Antenna (2016)

A frequency- and pattern-reconfigurable antenna based on a center-shorted microstrip patch is presented. The novel design utilizes two resonance modes of a microstrip antenna with shorting vias at the patch center. To set up the antenna tuning mechanism, two groups of varactors with a measured tuning range of [0.149, 1.304] pF are placed at two opposite sides of the antenna, followed by open-circuited loading stubs. For a particular bias voltage configuration, the structure operates as a dual-band antenna with broadside radiation at the upper resonance frequency and monopole-like radiation at the lower band. By varying the dc bias voltage, both resonance frequencies can be changed simultaneously. Based on the proposed concept, a demonstration antenna has been designed so that the two types of aforementioned patterns can be reconfigured across a continuous fractional frequency range of more than 20%. Experimental results are provided, which validate the proposed concept and design procedure.
NSAIDs and fracture healing (2013)

These animal data, together with the view of limited scientifically robust clinical evidence in humans, indicate that physicians consider only short-term administration of COX-2 inhibitors or other drugs in the pain management of patients who are in the phase of fracture or other bone defect healing. COX-2-inhibitors should be considered a potential risk factor for fracture healing, and therefore to be avoided in patients at risk for delayed fracture healing.
Electrical characterization of dielectrically isolated silicon substrates containing buried metallic layers (1997)

Dielectrically isolated substrates containing buried highly conducting WSi <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> layers are characterized for the first time using MOS capacitors. The active silicon layer is approximately 3 μm thick with a buried WSi <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> layer adjacent to the isolation layer. The buried metal forms the back contact of the capacitor and excellent MOS characteristics are observed. Minority carrier lifetimes in excess of 200 μs were measured indicating the suitability of these substrates for use in device manufacture.
Outer shell actuator driving central bending shaft by balloon arrays circumferentially-arranged inside of shell (2009)

This paper proposes a novel outer shell actuator having circumferentially-arranged micro balloon arrays on an inner wall of the shell. The actuator can drive central bending shaft through the cylindrical shell by swelling pneumatic balloon arrays. Surrounding balloons push the central shaft independently. It is possible to manipulate a head of the shaft by controlling individual deformation of surrounding balloons. The outer shell actuator is designed and developed in order to manipulate a medical forceps for endoscopic surgery as a practical example of the central shaft. Manipulating of forceps requires manipulating whole endoscope, which causes undesired motion of other medical tools for the endoscope such as a camera. Presented outer shell actuator allows independent manipulation of forceps.
Coincidence of pro- and anti-inflammatory responses in the early phase of severe sepsis: Longitudinal study of mononuclear histocompatibility leukocyte antigen-DR expression, procalcitonin, C-reactive protein, and changes in T-cell subsets in septic and postoperative patients (2002)

Decreases in monocytic HLA-DR expression occurred simultaneously with signs of hyperinflammation as early as the onset of severe sepsis and usually developed in opposite directions than inflammatory markers and sepsis severity scores.
Electro-optic effects in thin-film lanthanum-doped lead zirconate titanate (1990)

We report the deposition and characterization of highly oriented thin-film lanthanum-doped lead zirconate titanate (28/0/100) on fused-silica substrates by rf planar magnetron sputtering. The quadratic electro-optic effect gives a birefringence of Deltan ~ 0.018 at an applied field of 39 kV/mm. Optical modulation at a switching speed of 3 nsec (instrumentation limited) is achieved. Results of optically induced decay of the birefringence in these films are also reported.
Quantitation and kinetics of polymorphonuclear leukocyte and lymphocyte accumulation in joints during adjuvant arthritis in the rat. (1991)

Infiltration of polymorphonuclear leukocytes (PMNL) and lymphocytes into joints is a prominent feature of human and experimental arthritis. Here we evaluated the usefulness of techniques previously employed for measuring blood PMNL and small, inflammatory site-seeking T-lymphocytes, used in models of dermal inflammation, to monitor the migration of these leukocytes into the joints of rats with adjuvant arthritis. One week after immunization of rats with adjuvant (Mycobacterium butyricum in oil), 51Cr-labeled rat blood PMNL migrated into the hind- and forelimb joints, 5- to 7-fold more than in control animals. This preceded 111In-labeled lymphocyte accumulation, plasma 125I-labeled albumin extravasation, and clinical disease by 4 to 6 days. At 2 weeks, PMNL accumulation in joints increased further, and T-lymphocyte accumulation increased to 6 times that in control animals. PMNL localization in joints maximized at 3 weeks, reaching 20 to 35 times that in control animals; T-lymphocyte accumulation plateaued between 2 and 3 weeks; and all parameters tended to decline by 4 weeks. Migration of T lymphocytes to lymph nodes and to skin inflammatory sites was normal, whereas PMNL migration to zymosan activated serum (C5adesArg) was increased in rats with adjuvant arthritis. Treatment of arthritic rats with dexamethasone (1 mg/kg for 2 to 3 days) caused a dramatic inhibition of plasma albumin extravasation and PMNL migration into arthritic joints and improved clinical scores. In contrast, small T-lymphocyte migration into the joints or into lymph nodes was not inhibited even though lymphocyte migration in the same animals into dermal inflammatory reactions induced by interferon gamma, tumor necrosis factor-alpha, endotoxin, and polyinosine-cytosine was markedly suppressed. These results indicate distinctive patterns of lymphocyte migration into arthritic joint inflammation as compared with dermal inflammation. The experiments demonstrate that the radiolabeled PMNL and lymphocyte migration assays employed here are sensitive and reproducible and allow simultaneous quantitation of leukocyte infiltration during arthritis. These techniques should be useful for studies of the mechanisms involved in leukocyte migration in arthritis and the modulation of joint inflammation.
Irreversible and highly enantioselective acylation of 2-halo-1-arylethanols in organic solvents catalyzed by a lipase from Pseudomonas fluorescens (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIrreversible and highly enantioselective acylation of 2-halo-1-arylethanols in organic solvents catalyzed by a lipase from Pseudomonas fluorescensJun Hiratake, Minoru Inagaki, Takaaki Nishioka, and Junichi OdaCite this: J. Org. Chem. 1988, 53, 26, 6130–6133Publication Date (Print):December 1, 1988Publication History Published online1 May 2002Published inissue 1 December 1988https://pubs.acs.org/doi/10.1021/jo00261a033https://doi.org/10.1021/jo00261a033research-articleACS PublicationsRequest reuse permissionsArticle Views795Altmetric-Citations96LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts