gebhart/patrepeval huggingface dataset:
Myofibrillar calcium sensitivity of isometric tension is increased in human dilated cardiomyopathies: role of altered beta-adrenergically mediated protein phosphorylation. (1996)

To examine the role of alterations in myofibrillar function in human dilated cardiomyopathies, we determined isometric tension-calcium relations in permeabilized myocytesized myofibrillar preparations (n = 16) obtained from left ventricular biopsies from nine patients with dilated cardiomyopathy (DCM) during cardiac transplantation or left ventricular assist device implantation. Similar preparations (n = 10) were obtained from six normal hearts used for cardiac transplantation. Passive and maximal Ca2+-activated tensions were similar for the two groups. However, the calcium sensitivity of isometric tension was increased in DCM compared to nonfailing preparations ([Ca2+]50=2.46+/-0.49 microM vs 3.24+/-0.51 microM, P < 0.001). In vitro treatment with the catalytic subunit of protein kinase A (PKA) decreased calcium sensitivity of tension to a greater degree in failing than in normal preparations. Further, isometric tension-calcium relations in failing and normal myofibrillar preparations were similar after PKA treatment. These findings suggest that the increased calcium sensitivity of isometric tension in DCM may be due at least in part to a reduction of the beta-adrenergically mediated (PKA-dependent) phosphorylation of myofibrillar regulatory proteins such as troponin I and/or C-protein.
Biochemical Composition and Electrolyte Balance of "Unstimulated" Whole Human Saliva (2000)

The biochemical composition of "unstimulated" whole saliva was determined in healthy adult subjects. Based on their relative concentration, salivary analytes could be classified into three arbitrary categories: concentration lower than in serum (saliva/serum ratio < 0.5; 12 analytes), similar to serum (ratio = 0.5-1.5; five analytes), and higher than in serum (ratio > 1.5; five analytes). Consistent with local production, an elevated lactate dehydrogenase (LDH) activity in the saliva was associated with a non-serum like LDH isoenzyme pattern: LDH5 >> LDH4 > LDH3 >> LDH2 > LDH1. Compared with serum, the concentrations of hydrogen (as reflected in the pH), potassium and inorganic phosphorus were much higher (saliva/serum ratio > or = 3), whereas that of sodium, total magnesium, chloride, and total carbon dioxide were lower (saliva/serum ratio < or = 0.3). The concentration of ionized calcium was similar in saliva and serum (saliva/serum ratio = 0.8), while ionized magnesium was unmeasurable in saliva. The salivary ionized calcium fraction was higher (0.76) than previously suggested (0.51). The difference between the main salivary cations (potassium, sodium), and anions (phosphate, chloride) was similar to serum (anion gap: 4 vs. 11 meq/l). Highly significant (p < or = 0.012) correlations occurred among salivary pH, dihydrophosphate, total calcium, and potassium. Our data suggest that calcium, potassium, chloride and phosphates are the major salivary complex-forming ions. The major compositional differences between serum and saliva show that saliva is not a passive "ultrafiltrate" of serum and salivary constituents may play a distinct physiological role.
Soluble mouse major histocompatibility complex class II molecules produced in <i>Drosophila</i> cells (1995)

We have exploited Drosophila melanogaster Schneider cells and compatible inducible expression vectors to produce large amounts of secreted major histocompatibility complex (MHC) class II molecules (I-Ed). A simple two-step purification protocol was developed. In the first step, recombinant molecules were enriched using a monoclonal anti-class II antibody column followed by a nickel chelate column which further purified and concentrated the recombinant protein to several mg/ml. Characterization of the purified material indicates that the molecules are correctly assembled into alpha beta heterodimers. Further analysis shows that the recombinant MHC class II molecules are devoid of endogenous peptides and, therefore, homogeneous peptide/MHC complexes could be prepared by adding exogenous I-Ed-specific peptides at slightly acidic pH. Upon peptide addition, molecules underwent a conformational change into a more compact form revealed by gel filtration analysis. In addition, the peptide/MHC complexes were biologically active. As little as 10 ng of these complexes coated on plastic from a 100 ng/ml solution were sufficient to trigger antigen-specific T cell hybridomas. These MHC class II molecules, together with various forms of soluble T cell receptor (TcR) proteins, provide valuable tools to analyze the molecular details of TcR/antigen recognition.
Regulation, linkage, and sequence of mouse metallothionein I and II genes. (1984)

The mouse metallothionein II (MT-II) gene is located approximately 6 kilobases upstream of the MT-I gene. A comparison of the sequences of mouse MT-I and MT-II genes (as well as those of other mammals) reveals that the coding regions are highly conserved even at "silent" positions but that the noncoding regions and introns are extremely divergent between primates and rodents. There are four blocks of conserved sequences in the promoters of mouse MT-I, mouse MT-II, and human MT-IIA genes; one includes the TATAAA sequence, and another has been implicated in regulation by heavy metals. Mouse MT-I and MT-II mRNAs are induced to approximately the same extent in vivo in response to cadmium, dexamethasone, or lipopolysaccharide. Mouse MT-I and MT-II genes are regulated by metals but not by glucocorticoids after transfection into HeLa cells.
The effects of nitrogen and silicon profile on high-k MOSFET performance and Bias Temperature Instability (2004)

Nitrogen profile has been modulated by inserting Si layer into HfO/sub x/N/sub y/. In this paper, the effects of nitrogen and silicon on MOSFET performance and BTI (Bias Temperature Instability) characteristics have been investigated. Nitrogen incorporation enhanced V/sub TH/ shift for both PBTI (Positive Bias Temperature Instability) and NBTI (Negative Bias Temperature Instability). However, BTI degradation is significantly suppressed by the Si insertion. This improvement can be attributed to the reduction of oxide bulk trapped as well as interface trapped charge generation resulting from the insertion of Si layer.
miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression (2014)

miRNAs are short, noncoding RNAs that regulate expression of target genes at post-transcriptional levels and function in many important cellular processes, including differentiation, proliferation, etc. In this study, we observed down-regulation of miR-199a-5p during monocyte/macrophage differentiation of HL-60 and THP-1 cells, as well as human CD34(+) HSPCs. This down-regulation of miR-199a-5p resulted from the up-regulation of PU.1 that was demonstrated to regulate transcription of the miR-199a-2 gene negatively. Overexpression of miR-199a-5p by miR-199a-5p mimic transfection or lentivirus-mediated gene transfer significantly inhibited monocyte/macrophage differentiation of the cell lines or HSPCs. The mRNA encoding an ACVR1B was identified as a direct target of miR-199a-5p. Gradually increased ACVR1B expression level was detected during monocyte/macrophage differentiation of the leukemic cell lines and HSPCs, and knockdown of ACVR1B resulted in inhibition of monocyte/macrophage differentiation of HL-60 and THP-1 cells, which suggested that ACVR1B functions as a positive regulator of monocyte/macrophage differentiation. We demonstrated that miR-199a-5p overexpression or ACVR1B knockdown promoted proliferation of THP-1 cells through increasing phosphorylation of Rb. We also demonstrated that the down-regulation of ACVR1B reduced p-Smad2/3, which resulted in decreased expression of C/EBPα, a key regulator of monocyte/macrophage differentiation, and finally, inhibited monocyte/macrophage differentiation.
Richard M. Bozorth (1981)

cavity referenced (for ESR) Model 210C...
Focused Ion Beam Fabrication of Graded Channel Field Effect Transistors (FETs) in GaAs and Si (1988)

Abstract : The aim of our focused ion beam research program at M.I.T. is to exploit the unique capabilities of this novel technology in fabricating devices which have a lateral gradient of doping. (This program of research will be continued so that this final report will be exhaustive.) Using internal institute funds, M.I.T. purchased a focused ion beam system capable of producing beams of the common dopants of Si and GaAs (i.e., B, Si, and Be) at energies up to 300 keV. In order to carry out the proposed implantations the system needed to be upgraded in several ways. Pattern writing software was developed, as well as software for translating patterns written in MAGIC, a layout system in use at M.I.T., into the FIB format. To achieve the minimum beam diameter the ion column had to be realigned mechanically and electrical noise needed to be reduced in the circuits which deflect the beam. With the upgraded system we have written patterns in PMMA with 50 nm line width, and achieved metal lift-off of 50 nm width lines.
Gene discovery in rheumatoid arthritis highlights the CD40/NF‐ĸB signaling pathway in disease pathogenesis (2009)

During the past several years, substantial progress has been made in the identification of genetic variants that predispose to rheumatoid arthritis (RA) and other autoimmune disorders. Progress in this area has been facilitated by the availability of new technology that allows for a much more comprehensive screen of the genome than was possible before, in conjunction with large samples of RA patients with well-characterized disease. Recent RA genetic studies have identified genes with important functions related to intracellular signaling mechanisms, transcription factors, cytokines, membrane receptors, costimulatory molecules, and enzymes. In particular, recent discoveries highlight the importance of the CD40/NF-kappaB signaling pathway in RA, based on genetic association with several genes relevant to this pathway, including CD40, TRAF1, TNFAIP3, and REL. Progress in the identification of genes that contribute to RA is proceeding at a very rapid pace. These genetic discoveries shed light on underlying disease mechanisms in RA and provide targets for the development of novel diagnostic and therapeutic tools for future use in this chronic inflammatory disorder.
Accreditation in business education (1999)

Business school accreditation has been long established in North America. Its development in the UK and Europe is of much more recent origin and has been fuelled primarily by concerns to protect the MBA brand. It is only recently that a European process of whole school accreditation (EQUIS) has emerged, and the process is now continuing with the “second wave” of schools. This paper traces the development of accreditation processes in the USA, the UK, across the European Union and Central and Eastern Europe. It also explores the implications of accreditation and recognition systems for providers and consumers of business qualifications, whether employers or prospective students. The relationship between these systems and national and institutional approaches to academic quality standards is explored. Finally, we analyse the future of the range of accrediting systems in the UK and the wider European Union.
Errors in Inhalation Technique and Efficiency in Inhaler Use in Asthmatic Children (1986)

256 asthmatic children receiving regular inhalation therapy demonstrated how they used their inhalers. Pulmonary function measurements (PFM) were made before and after the demonstrations, and errors in technique were recorded. 242 children had reversible airway obstruction on the day of study. In only 109 (45%) did the inhalation result in an increase in FEV1 greater than or equal to 15% (efficient technique). An efficient inhalation technique was found in 46% of children who demonstrated a pressurized aerosol, 59% who demonstrated a tube spacer aerosol and 46% who demonstrated a rotahaler, and the frequency of efficient technique varied from 17% to 84% between six different groups of instructors. 87% of children controlled and 25% not controlled with PFM at the time of prescription had an efficient technique. Children under 6 years had a more inefficient and a more faulty technique than older children, but otherwise age did not influence the result. Neither was time since instruction of any importance for efficiency or number of errors. The errors recorded that seem to influence efficiency most were: coordination problems, rapid inspirations, ceasing to inspire when the aerosol was fired, and inhalation through the nose. The results emphasize the paramount importance of clear instructions and control of inhalation technique at the time the treatment is prescribed.
Control of delivered gene expression in chondrocytes using heat shock protein 70B promoter. (1999)

HSP70B promoter could regulate the expression of delivered genes according to the intensity of heat stress.
Complete Genome Sequence of a Severe Acute Respiratory Syndrome-Related Coronavirus from Kenyan Bats (2019)

We identified a strain of betacoronavirus BtKY72/<i>Rhinolophus</i> sp./Kenya/2007 (here BtKY72) from rectal swab samples in Kenyan bats. This paper reports the complete genomic sequence of BtKY72, which is closely related to BtCoV/BM48-31/Bulgaria/2008, a severe acute respiratory syndrome (SARS)-related virus from <i>Rhinolophus</i> bats in Europe.
A simplified formula for viscous and chemical absorption in sea water (1998)

A simplified expression is presented for predicting the absorption of sound in sea water, retaining the essential dependence on temperature, pressure, salinity, and acidity of the more complicated formula on which it is based [R. E. Francois and G. R. Garrison, “Sound absorption based on ocean measurements. Part II: Boric acid contribution and equation for total absorption,” J. Acoust. Soc. Am. 72, 1879–1890 (1982)]. The accuracy of the simplified formula is demonstrated by comparison with the original one for a range of oceanographic conditions and frequencies between 100 Hz and 1 MHz.
High dielectric constant rutile–polystyrene composite with enhanced percolative threshold (2012)

TiO2 (rutile) nanocrystals, obtained by hydrothermal synthesis, are coated with polystyrene, grown by RAFT polymerization, and are dispersed into a polystyrene matrix at various concentrations. The morphology of both the polystyrene coating shell and TiO2 filler particles dispersed in the polymeric matrix is investigated. The polymer molecules attached to the surfaces of TiO2 nanoparticles exist in a “brush” regime; rutile nanoparticles self-assemble in chestnut-burr aggregates whose number increases with the filler amount. By increasing the filler concentration, the composites display a high dielectric constant, which is ascribed to the self-assembling of rutile nanoparticles in chestnut-burr aggregates, where a number of rutile crystals share the lateral faces and form capacitive microstructures. The crystals in these aggregates are separated by a polymer thin layer and allow a high percolative threshold, 41% v/v of filler amount, before the formation of a continuous network responsible for the sudden change of the dielectric characteristics. Despite the high content of inorganic filler, the dissipation factor remains low, even approaching the lower frequencies. The material is easily processable because of its polymeric nature and good reproducibility, thanks to the morphology control of the filler particles and their aggregates.
Don’t ignore the green light: exploring diverse roles in plant processes (2017)

The pleasant green appearance of plants, caused by their reflectance of wavelengths in the 500-600 nm range, might give the impression that green light is of minor importance in biology. This view persists to an extent. However, there is strong evidence that these wavelengths are not only absorbed but that they also drive and regulate physiological responses and anatomical traits in plants. This review details the existing evidence of essential roles for green wavelengths in plant biology. Absorption of green light is used to stimulate photosynthesis deep within the leaf and canopy profile, contributing to carbon gain and likely crop yield. In addition, green light also contributes to the array of signalling information available to leaves, resulting in developmental adaptation and immediate physiological responses. Within shaded canopies this enables optimization of resource-use efficiency and acclimation of photosynthesis to available irradiance. In this review, we suggest that plants may use these wavelengths not just to optimize stomatal aperture but also to fine-tune whole-canopy efficiency. We conclude that all roles for green light make a significant contribution to plant productivity and resource-use efficiency. We also outline the case for using green wavelengths in applied settings such as crop cultivation in LED-based agriculture and horticulture.
Anchoring fibrils contain the carboxyl-terminal globular domain of type VII procollagen, but lack the amino-terminal globular domain. (1987)

Type VII procollagen has been characterized as a product of epithelial cell lines. As secreted, it contains a large triple-helical domain terminated by a multi-globular-domained carboxyl terminus (NC-1), and a smaller amino-terminal globule (NC-2). The triple helix and the NC-1 domain have previously been identified in anchoring fibril-containing tissues by biochemical and immunochemical means, leading to the conclusion that type VII collagen is a major component of anchoring fibrils. In order to better characterize the tissue form of type VII collagen, we have produced a panel of monoclonal antibodies which recognize the NC-1 domain. Peptide mapping of these epitopes indicate that they are independent and span approximately 125,000 kDa of the total 150,000 kDa of each alpha chain contained in NC-1. All these antibodies elicit immunofluorescent staining of the basement membrane zone in tissues. Type VII collagen has been extracted from tissues. As previously reported, it is smaller than type VII procollagen, (Woodley, D. T., Burgeson, R. E., Lunstrum, G. P., Bruckner-Tuderman, L., and Briggaman, R. A., submitted for publication), and we now find that it predominantly occurs as a dimer. Following clostridial collagenase digestion, intact NC-1 has been recognized, indicating that the difference in apparent Mr between the tissue form of the molecule and type VII procollagen results from modification of the amino terminus. The size of the amino-terminal globule has been determined to be between approximately 96 and 102 kDa. Rotary shadowing analyses of extracted molecules indicate that dimeric molecules contain the NC-1 domain, but are missing intact NC-2. We propose that the tissue form monomer, Mr = 960,000, be referred to as "type VII collagen." These studies strongly suggest that anchoring fibrils contain dimeric molecules with intact NC-1 domains. The data also support the previous suggestion that the NC-2 domain is involved in the formation of disulfide bond-stabilized type VII collagen dimers, and is subsequently removed by physiological proteolytic processing.
Dynamic behaviours of piston rings and their practical impact. Part 2: Oil transport, friction and wear of ring/liner interface and the effects of piston and ring dynamics (2002)

The paper discusses several important processes that have great impact on the lubrication between the top two rings and the liner. The analysis is conducted on the basis of the calculation results on a heavy-duty (HD) diesel engine using theoretical models. Oil supply mechanisms to different liner regions are analysed, and emphasis is given to the oil transport to the top liner region that is found critical to friction, wear and oil consumption in HD diesel engines. Additionally, the paper discusses the oil supply to the second ring, its uncertainties and the effect on the prediction of the performance of the top two rings. Furthermore, the effects of dynamics of the piston and rings on friction, wear and oil transport are illustrated and the effects of bore distortion on oil transport are discussed. For practical purposes, a formula to describe the second ring running surface profile is given based on simple geometrical constraint. A new truncation method is rendered for plateau surface roughness in order to effectively use the existing mixed lubrication models.
Randomized, Double-Blind, Phase III Trial of Ipilimumab Versus Placebo in Asymptomatic or Minimally Symptomatic Patients With Metastatic Chemotherapy-Naive Castration-Resistant Prostate Cancer (2016)

Purpose Ipilimumab increases antitumor T-cell responses by binding to cytotoxic T-lymphocyte antigen 4. We evaluated treatment with ipilimumab in asymptomatic or minimally symptomatic patients with chemotherapy-naive metastatic castration-resistant prostate cancer without visceral metastases. Patients and Methods In this multicenter, double-blind, phase III trial, patients were randomly assigned (2:1) to ipilimumab 10 mg/kg or placebo every 3 weeks for up to four doses. Ipilimumab 10 mg/kg or placebo maintenance therapy was administered to nonprogressing patients every 3 months. The primary end point was overall survival (OS). Results Four hundred patients were randomly assigned to ipilimumab and 202 to placebo; 399 were treated with ipilimumab and 199 with placebo. Median OS was 28.7 months (95% CI, 24.5 to 32.5 months) in the ipilimumab arm versus 29.7 months (95% CI, 26.1 to 34.2 months) in the placebo arm (hazard ratio, 1.11; 95.87% CI, 0.88 to 1.39; P = .3667). Median progression-free survival was 5.6 months in the ipilimumab arm versus 3.8 with placebo arm (hazard ratio, 0.67; 95.87% CI, 0.55 to 0.81). Exploratory analyses showed a higher prostate-specific antigen response rate with ipilimumab (23%) than with placebo (8%). Diarrhea (15%) was the only grade 3 to 4 treatment-related adverse event (AE) reported in ≥ 10% of ipilimumab-treated patients. Nine (2%) deaths occurred in the ipilimumab arm due to treatment-related AEs; no deaths occurred in the placebo arm. Immune-related grade 3 to 4 AEs occurred in 31% and 2% of patients, respectively. Conclusion Ipilimumab did not improve OS in patients with metastatic castration-resistant prostate cancer. The observed increases in progression-free survival and prostate-specific antigen response rates suggest antitumor activity in a patient subset.