gebhart/patrepeval huggingface dataset:
Identification of Androgen-Regulated Genes in Mouse Kidney by Representational Difference Analysis and Random Arbitrarily Primed Polymerase Chain Reaction<sup>1</sup> (1998)

The molecular nature of tissue-specific gene regulation by androgens has not been well defined, partly as a result of the variable expression and incomplete regulation of currently available gene models. We have therefore aimed to establish more informative models by identifying alternative genes whose expression is tightly and coordinately regulated by androgens. Female C57BL/6 mice were dosed with dihydrotestosterone- or sham-treated for 8 days, after which kidneys were removed and complementary DNA (cDNA) prepared. We then applied the subtractive hybridization techniques of random arbitrarily primed-PCR and PCR-coupled subtractive hybridization method of cDNA representational difference analysis to the isolated cDNA. In addition to well characterized androgen-regulated genes [e.g. KAP (kidney androgen-regulated protein)], we demonstrate the differential expression of six genes previously not known to be under androgen control. RNA levels of SA, Cytochrome P450 4B1, IL-6ST (interleukin-6 signal transducer), OATP (organic anion transporter), and a newly identified gene, MJAM, were up-regulated by androgen, while 16-alpha-hydroxylase was decreased. Expression of these transcripts was inhibited in dihydrotestosterone-treated females by flutamide and in males by castration, confirming their dependence on androgens. Although all the genes demonstrate tissue-specific regulation by androgen, SA showed both kidney specificity and absolute requirement for androgen for its expression. These newly identified androgen-regulated genes will constitute very useful models for studying the nature of tissue-specific gene regulation by androgens.
Stretching single molecular DNA by temperature gradient (2007)

The authors present a method to control the conformation of DNA by using temperature gradient. The conformations of one end tethered and two ends tethered DNA are measured in different temperature gradients up to 3K∕μm. The results show that temperature gradient can exert force on a single DNA and create internal tension within it. The magnitude of the force is of the order of 0.1pN and is enough to manipulate and stretch DNA. This way of manipulating DNA requires no beads and provides local control, while none of the other methods can satisfy these two requirements at the same time.
[In vitro activities of 23 antimicrobial agents against 4,993 gram-positive and gram-negative bacterial strains isolated from multicenter of Japan during 1994--in vitro susceptibility surveillance.Levofloxacin-Surveillance Group]. (1999)

In a surveillance study conducted during 1994 at 24 medical institutes from different geographical areas of Japan, the susceptibility of clinical isolates to twenty three comparative agents, such as ofloxacin, levofloxacin, ciprofloxacin, tosufloxacin, ampicillin, clavulanic acid/amoxicillin, oxacillin, piperacillin, cefaclor, cefotiam, cefdinir, cefclidin, ceftazidime, cefpirome, imipenem, aztreonam, vancomycin, minocycline, chloramphenicol, clarithromycin, sulfamethoxazole/trimethoprim, amikacin, and gentamicin, were tested by the standard broth micro-dilution method. A total of 4,993 isolates tested in this study included Streptococcus pneumoniae, methicillin susceptible Staphylococcus aureus (MSSA), methicillin resistant Staphylococcus aureus (MRSA), coagllase negative streptococci (CNS), Enterococcus faecalis, Enterococcus faecium, Enterobactericeae, Pseudomonas aeruginosa from patients with urinary tract infections or respiratory tract infections, and Haemophilus influenzae. For MSSA, S. pneumoniae, Enterobacteriaceae, and H. influenzae, more than 70% of the isolates was susceptible to fluoloquinolones. However, resistance occurred in more than 50% of MRSA and P. aeruginosa isolated from UTI. Fluoroquinolones were found to be effective against high level penicillin-resistant S. pneumoniae, the third generation cephem-resistant Enterobacteriaceae and ampicillin-resistant H. influenzae.
Passivation of Sodium Ions in Metal Oxide Semiconductor Structures by Annealing with Ultraviolet Light (1996)

The passivation effect of ultraviolet light annealing on sodium‐contaminated metal oxide semiconductor (MOS) devices was studied. MOS devices were intentionally contaminated with sodium during the interconnect formation process after the gate electrode was formed. After aluminum pad formation, these devices were irradiated with ultraviolet light (wavelength 250 to 370 nm) at elevated temperatures. Sodium‐induced threshold voltage of active and parasitic MOS transistors returned to original values during the annealing. It is assumed that positive sodium ions in the oxides are neutralized with electrons excited by ultraviolet light. This passivation effect becomes more prominent as the temperature becomes higher, suggesting that the diffusion of sodium ions in the oxides is closely related to the passivation effect. A model for this temperature‐dependent passivation effect is presented. Bias‐temperature testing revealed that this passivation effect is remarkably stable for 100 h even at 200°C.
Both Interleukin 4 and Interleukin 13 Induce Tyrosine Phosphorylation of the 140-kDa Subunit of the Interleukin 4 Receptor (1995)

We have investigated tyrosine phosphorylation of cellular proteins induced by interleukin (IL) 4 and compared it with the effects of three related cytokines, IL-2, IL-7, and IL-13. We show here that both IL-4 and IL-13 stimulate tyrosine phosphorylation of the 140-kDa IL-4 receptor subunit, which suggests that this receptor protein is used by both cytokines. Receptor phosphorylation induced by IL-13 was both weaker and slower than with IL-4. Stimulation of cells with IL-2 and IL-7 induced identical phosphorylation patterns to each other but not phosphorylation of the 140-kDa IL-4 receptor subunit. The only signal appearing upon stimulation with any of the four cytokines was the weak phosphorylation of an unidentified protein of 160 kDa. SH2 domains of p56lck and p59fyn precipitated the same proteins as anti-phosphotyrosine antibodies after IL-4 stimulation, which suggests that a src-type kinase may be involved in signal transduction through the IL-4 receptor.
Management of Status Epilepticus (1982)

IN 1824 Calmeil first described "grand mal" status epilepticus in his thesis, De l'Epilepsie.1 He had observed patients who had repeated tonic-clonic seizures and did not recover consciousness between attacks. Fifty-two years later, Bourneville referred to status epilepticus as "etat de mal" in his book published in 1876.2 The full meaning of "status epilepticus," however, was not fully appreciated until 1962, when it was defined as "epileptic seizures that are so frequently repeated or so prolonged as to create a fixed and lasting epileptic condition."3 The term was applied to continuous seizures lasting at least 30 minutes, even when . . .
Investigation of Q-learning applied to DVFS management of a System-on-Chip (2016)

This paper presents a new Q-learning based strategy to manage Dynamic Voltage Frequency Scaling (DVFS) on a system on chip (SoC) such that the energy consumption is reduced. We address software applications with throughput constraints. The proposed Q-learning formulation has two main advantages: it has a reduced state space to limit the overhead and it embeds a new reward function tailored for throughput-constrained applications. The DVFS manager is evaluated on a test chip executing an HMAX object recognition application. We perform an experimental investigation of the main Q-learning parameters. The results suggest that the proposed method reduces the energy consumed with up to 44% at the cost of occasionally increasing the number of throughput violations, when compared to two state-of-the-art feedback controllers that address the same application domain.
Mammographic Images Enhancement and Denoising for Breast Cancer Detection Using Dyadic Wavelet Processing (2008)

Mammography is the most effective method for the early detection of breast diseases. However, the typical diagnostic signs such as microcalcifications and masses are difficult to detect because mammograms are low-contrast and noisy images. In this paper, a novel algorithm for image denoising and enhancement based on dyadic wavelet processing is proposed. The denoising phase is based on a local iterative noise variance estimation. Moreover, in the case of microcalcifications, we propose an adaptive tuning of enhancement degree at different wavelet scales, whereas in the case of mass detection, we developed a new segmentation method combining dyadic wavelet information with mathematical morphology. The innovative approach consists of using the same algorithmic core for processing images to detect both microcalcifications and masses. The proposed algorithm has been tested on a large number of clinical images, comparing the results with those obtained by several other algorithms proposed in the literature through both analytical indexes and the opinions of radiologists. Through preliminary tests, the method seems to meaningfully improve the diagnosis in the early breast cancer detection with respect to other approaches.
Cytoplasmic Delivery of Liposomes into MCF-7 Breast Cancer Cells Mediated by Cell-Specific Phage Fusion Coat Protein (2010)

Earlier, we have shown that doxorubicin-loaded liposomes (Doxil) modified with a chimeric phage fusion coat protein specific toward MCF-7 breast cancer cells identified from a phage landscape library demonstrated a significantly enhanced association with target cells and an increased cytotoxicity. Based on some structural similarities between the N-terminus of the phage potein and known fusogenic peptides, we hypothesized that, in addition to the specific targeting, the phage protein may possess endosome-escaping potential and an increased cytotoxicity of drug-loaded phage protein-targeted liposomes may be explained by an advantageous combination of both, cell targeting and endosomal escape of drug-loaded nanocarrier. The use of the fluorescence resonance energy transfer (FRET) technique allowed us to clearly demonstrate the pH-dependent membrane fusion activity of the phage protein. Endosomal escape and cytosolic delivery of phage-liposomes was visualized with fluorescence microscopy. Endosome acidification inhibition by bafilomycin A 1 resulted in decreased cytotoxicity of the phage-Doxil, while the endosome disruption by chloroquine had a negligible effect on efficacy of phage-Doxil, confirming its endosomal escape. Our results demonstrated an endosome-escaping property of the phage protein and provided an insight on mechanism of the enhanced cytotoxicity of phage-Doxil.
China, Asia, and the World Economy： The Implications of an Emerging Asian Core and Periphery (2006)

证据的成长身体建议中国的出现在亚洲的先进、开发的乡村上正在有微分效果。这个区域的先进乡村为他们的大写的商品，部件和技术正在得益于一个大、很快成长的中国市场的存在，而它的发展中的乡村在第三个市场与中国面对面竞争。这些事实为寻求赶上这个区域的工业领导人的迟了使工业化的亚洲乡村创造另外的挑战。接着，一个亚洲核心和圆周的出现不鼓励一个连贯的亚洲经济、政治的团体的发展。亚洲 regionalism 将是开的 regionalism 以便阻止地区性的行动产生昂贵的贸易转向。支持亚洲金融开发和集成的努力是不大可能的与世界的其余部分在损坏这个区域的金融连接的情况下来。向有联系普通钱的位置的普通汇率政体的一个早行动将是有问题的。并且当这些普通政策不在时，迫使让强大的地区性的机构的发展提出普通钱的位置将最多谦虚。
Spruce Budworm Egg Mass Density on Balsam Fir and White Spruce: Low Population Levels (Lepidoptera: Tortricidae) (2017)

As part of a study to develop improved foliage sampling methods for spruce budworm, Choristoneura fumiferana (Clemens), egg masses, two balsam fir (four in one stand), Abies balsamea, and two white spruce, Picea glauca, trees were chosen from each of five spruce- fir stands in Michigan's Upper Peninsula in 1980. All stands had very low to low population densities. Each tree was completely enumerated so that the number of new egg masses, foliage surface area, and egg mass density could be determined for the entire tree, three crown classes, four quadrants, and the tree top. Results indicated (1) considerable tree-to- tree and stand-to-stand variation; (2) no meaningful or consistent differences among quad- rants within or between species; (3) the average density in white spruce trees was 3.2 times larger than that in balsam fir trees; (4) the tree-la-tree variation of density in white spruce trees was 8.4 times larger than that in balsam fir trees; (5) densities in the mid-crown, upper-crown, and tree top are considerably higher than that in the lower-crown for both species; the relative differences for balsam fir are about twice that of white spruce; and (6) on the average, density at mid-crown was close to that of the entire tree for balsam fir, but density at mid-crown was 17.9% lower than that of the entire tree for white spruce. These results have important implications to the development of sampling plans for estimating egg mass density in spruce-fir stands.
High-sensitivity fiber-optic accelerometer (1989)

We describe a novel accelerometer in which the sensing element is a weighted diaphragm. The displacement of the diaphragm produced by acceleration is measured using a miniature hemispherical air-spaced Fabry-Perot interferometer, of which one mirror is mounted on the diaphragm. The interferometer is illuminated by a diode laser and addressed through a monomode optical fiber.
Preparation and Formulation of a Human Chorionic Gonadotropin Antifertility Vaccine: Selection of a Peptide Immunogen* (1981)

ABSTRACT: Peptides representing the amino acid sequence of the carboxy‐terminal of the human chorionic gonadotropin (HCG) beta subunit were used in studies to determine whether an immunogen could be prepared that would be suitable for an HCG antifertility vaccine. Peptides of varying length were conjugated to several macro‐molecular carriers, in varying peptide‐carrier ratios, via an N‐ or C‐terminal group, with and without amino acid spacers. Antibody levels to peptides and intact HCG were used as the criteria for conjugate utility. Immunizations with a peptide of 37 amino acid residues elicited the highest antibody levels to HCG. No differences were found between N‐ or C‐terminal conjugates, but peptides with amino acid spacers elicited higher responses than peptides without spacer. Conjugates with a peptide:carrier ratio greater than 20 peptides/10 5 daltons carrier were more immunogenic than those with a lower ratio. Diphtheria and tetanus toxoids were consistently the most effective carriers for enhancing antibody responses to peptides. It was concluded that conjugates of β‐HCG peptide 109–145 coupled to a toxoid carrier via its N‐terminus in a ratio of 20–30 peptides per 10 5 daltons carrier was a suitable immunogen for further studies for the development of an anti‐fertility vaccine.
Virtual Flow Meter pilot: based on Data Validation and Reconciliation Approach (2012)

Abstract To face the new challenges of production metering and back allocation, TOTAL is making an important effort to develop and test new approaches to improve its current production metering systems particularly for multiphase conditions. New technologies are tested through pilot applications within different subsidiaries worldwide. Thanks to these pilots, the suitability of various approaches to the needs of each asset can be analyzed and tested in detail. The SPE-149908 (M. Haouche 2012) paper presented a Virtual Flow Meter (VFM) field pilot in Qatar. The approach used on this VFM is the Data Validation and Reconciliation (DVR). The single Well model of the VFM is an assembly of three main units: Reservoir unit, Electrical Submersible Pump (ESP) unit and Production Tubing unit. The conclusion was that the VFM was catching-up the production trends of all the Wells of the pilot. However, the VFM results are not quantitatively comparable to the production measurements, especially when the VFM is running far away from the calibration period. A recommendation was to enhance the ESP model that controls mainly the information on the flow rate. The present paper describes a new method for modeling the ESP performances in the VFM. This method takes into account the impact of the Gas on the ESP performances. Results are presented for three operational cases to see the benefits of this method and where it is applicable. The ESP operational data have been considered for a long time period in order to get a representative range of operational conditions. Conclusions are made on the capabilities of the new method to assess the impact of Gas on the ESP operational performances.
Active vacuolar H+ATPase is required for both endocytic and exocytic processes during viral infection of BHK-21 cells (1994)

Bafilomycin A1 (Baf), a specific inhibitor of the vacuolar-type proton pump, inhibited the entry of Semliki Forest virus and vesicular stomatitis virus into BHK-21 cells. The inhibition occurred at concentrations that dissipated intracellular acidic compartments. Viral infection was totally inhibited by 30 nM Baf while endocytosis of the virus or fluorescein isothiocyanate-dextran was not affected. Thus, a vacuolar-type proton pump was responsible for acidification of the endosomes needed for virus entry. When the cells were exposed to 100 nM Baf after virus entry, viral glycoprotein synthesis continued normally. The viral glycoproteins acquired resistance to endoglycosidase H, indicating arrival in the medial Golgi apparatus. However, maturation processes occurring in the trans-Golgi compartment were inhibited, and the amounts of viral glycoproteins appearing at the cell surface were reduced. Double immunofluorescence studies showed that in the presence of Baf the viral glycoproteins were found in and around mannosidase II-positive Golgi structures. To analyze whether Baf blocked transport from the trans-Golgi compartment to the cell surface, the viral glycoproteins were allowed to accumulate in the trans-Golgi network by utilizing a 20 degrees C block. Subsequent incubation at 37 degrees C in the presence of Baf did not inhibit the terminal maturation processes or transport to the cell surface, suggesting that the block was before the trans-Golgi network. These results indicate that an active vacuolar-type proton pump in the Golgi apparatus is essential for protein transport in BHK-21 cells.
Foaming and Air Entrainment in Automatic Transmission Fluids (1976)

<div class="htmlview paragraph">The Ford Dynamic Aeration Test Apparatus is used to evaluate the foaming, air entrainment and air release properties of selected automatic transmission fluids. By this method it is possible to evaluate fluids under conditions which approach those of the automatic transmission operation.</div> <div class="htmlview paragraph">The effects of changes in aeration rate, temperature, pressure and air humidity on the foaming and air entrainment characteristics are evaluated and a rating procedure for automatic transmission fluid behavior is suggested.</div>
5-methoxyindole metabolites of L-tryptophan: control of COX-2 expression, inflammation and tumorigenesis (2014)

Cyclooxygenase-2(COX-2) overexpression promotes inflammation and tumorigenesis. COX-2 expression in response to diverse stimuli is tightly controlled to avoid persistent overexpression. 5-methoxyindole metabolites of L-tryptophan represent a new class of compounds that control COX-2 expression at the transcriptional level. Two of the metabolites, the newly discovered 5-methoxytryptophan (5-MTP, also known as cytoguardin) and N-acetyl 5-methoxytryptamine (melatonin) are the focus of this review. 5-MTP is produced by mesenchymal cells such as fibroblasts via 5-hydroxytryptophan (5-HTP). It inhibits COX-2 transcriptional activation induced by diverse proinflammatory and mitogenic factors. Cancer cells are deficient in cytoguardin production which contributes to COX-2 overexpression. Fibroblast-generated 5-MTP is capable of restoring the control of COX-2 overexpression in cancer cells. 5-MTP blocks cancer cell migration and invasion in vitro and inhibits tumor growth and cancer metastasis in a xenograft model. Melatonin possesses similar COX-2 suppressing and anti-cancer properties albeit at supra-pharmacological concentrations. By contrast, 5-hydroxyindole metabolites of L-tryptophan such as 5-hydroxytryptamine (serotonin), 5-hydroxytryptophol and other serotonin catabolites do not control COX-2 expression. 5-hydroxytryptophan inhibits COX-2 expression through conversion to 5-MTP. The physiological relevance of 5-MTP as an endogenous regulator of inflammation and cancer metastasis remains to be investigated. On the other hand, 5-methoxyindole metabolites of tryptophan are valuable lead compounds for development of new anti-inflammatory drugs and cancer chemoprevention.
Outer membrane proteins of Pseudomonas aeruginosa as vaccine candidates. (1994)

We tested the ability of recombinant outer membrane proteins of Pseudomonas aeruginosa to serve as a protective vaccine against this gram negative pathogen under two main pathophysiological events leading to P. aeruginosa sepsis. i) systemic infection during immunosuppression, and ii) bacterial translocation. A hybrid vaccine was cloned combining protective epitopes of outer membrane protein F (OprF) and outer membrane protein I (OprI). This vaccine proved to be highly protective against an intraperitoneal challenge with P. aeruginosa in immunosuppressed mice. Oral immunization of mice, with recombinant Salmonella dublin expressing OprI induced s-IgA antibodies in the gut mucosa against OprI and provided protection against translocation of P. aeruginosa in an immunosuppressed mouse model. To test whether OprI is safe for use in humans, recombinant OprI was purified and used for immunization of volunteers. Vaccination was well tolerated and no major side effects were observed. The induction of serum antibodies against OprI was found to be dose-dependent and was observed in total in 65% of the volunteers.
ARAP2 effects on the actin cytoskeleton are dependent on Arf6-specific GTPase-activating-protein activity and binding to RhoA-GTP (2006)

ARAP2 is a protein that contains both ArfGAP and RhoGAP domains. We found that it is a phosphatidylinositol (3,4,5)-trisphosphate-dependent Arf6 GAP that binds RhoA-GTP but lacks RhoGAP activity. In agreement with the hypothesis that ARAP2 mediates effects of RhoA, endogenous ARAP2 associated with focal adhesions (FAs) and reduction of ARAP2 expression, by RNAi, resulted in fewer FAs and actin stress fibers (SFs). In cells with reduced levels of endogenous ARAP2, FAs and SFs could be restored with wild-type recombinant ARAP2 but not mutants lacking ArfGAP or Rho-binding activity. Constitutively active Arf6 also caused a loss of SFs. The Rho effector ROKalpha was ineffective in restoring FAs. Conversely, overexpression of ARAP2 did not restore SFs in cells treated with a ROK inhibitor but induced punctate accumulations of paxillin. We conclude that ARAP2 is an Arf6GAP that functions downstream of RhoA to regulate focal adhesion dynamics.