gebhart/patrepeval huggingface dataset:
Antibodies specific for different T15 idiotopes induce neonatal suppression of the T15 idiotype (1983)

The response of BALB/c mice to Pneumococcus pneumoniae is dominated by a single idiotype defined by the phosphorylcholine-binding myeloma protein TEPC 15 (T15). Injection of newborn BALB/c mice with antibodies to the T15 idiotype will lead to suppression of this idiotype. Such mice will respond to the antigen Pneumococcus, but with idiotypically different anti-phosphorylcholine antibodies. In an attempt to further characterize the effect of anti-idiotypic antibodies on induction of tolerance, three monoclonal antibodies specific for different T15 idiotopes were used. Although these antibodies belonged to the mouse immunoglobulin classes, IgG1 or IgG2 alpha, and were derived from different mouse strains, in all cases, a long lasting suppression could be induced. The data show that injection of idiotope-specific antibodies will suppress the T15 idiotype, but not necessarily the idiotope itself. The results are discussed in terms of the mechanism which will lead to the suppression of a dominating idiotype in the newborn mouse.
Construction of phosphorylatable monoclonal antibody to a tumor-associated antigen. (1996)

A phosphorylation site was introduced into chimeric monoclonal antibody B72.3 (MAb-chB72.3) by site-specific mutation of the coding sequence. The phosphorylation site for the cAMP-dependent protein kinase was positioned at the carboxyl terminus of the heavy-chain constant region of MAb-chB72.3. The resultant modified MAb-chB72.3-P was expressed in 293 cells and purified. The MAb-chB72.3-P protein was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase with [gamma-32P]ATP to high radiospecific activity. The 32P-labeled MAb-chB72.3-P protein bound to cells expressing the tumor-associated glycoprotein 72 antigen. The introduction of phosphorylation sites into MAbs provides a new type of MAb for the diagnosis and treatment of cancers.
Isolation and characterization of a species-specific DNA probe for<i>Candida albicans</i> (1991)

As a model for the isolation of species-specific sequences of DNA, we isolated and characterized a species-specific DNA fragment from the Candida albicans genome. We used a series of differential colony hybridization experiments, in which a C. albicans genomic library was hybridized with genomic DNA probes from related organisms to minimize the number of potentially specific C. albicans DNA fragments to be tested. Six clones were tested by dot blot analysis, and one of them, a 1348 bp EcoRI DNA fragment, was confirmed as specific for C. albicans. This species-specific fragment could be utilized as a DNA probe for rapid, sensitive, and specific diagnosis of C. albicans DNA in clinical specimens.
The uPA/uPA receptor system as a target for tumor therapy (2001)

Invasiveness of a variety of tumors depends on the regulated expression of proteolytic enzymes that degrade the surrounding extracellular matrix and dissociate cell-cell and/or cell-matrix attachments. The tumor cell surface-associated urokinase-type plasminogen activator (uPA) system plays an especially important role in tumor cell invasion and metastasis. It consists of the serine protease uPA, its membrane-bound receptor (uPAR, CD87) and one of the natural inhibitors PAI-1 or PAI-2. There are strong indications based on animal experiments that interference with this system by inhibiting the enzymatic activity of uPA and/or antagonizing its binding to the receptor is of therapeutic relevance. With the recent solution of various X-ray structures of uPA/inhibitor complexes, structural information is available for optimizing existing lead compounds in their affinity and selectivity for uPA. Furthermore, peptide compounds capable of mimicking the structural epitope of uPA responsible for binding to the receptor efficiently antagonize this recognition process. Thus, both approaches prove to be well suited for the design of highly promising drugs in human medicine.
Experimental research on the rotating detonation in gaseous fuels–oxygen mixtures (2011)

An experimental study on rotating detonation is presented in this paper. The study was focused on the possibility of using rotating detonation in a rocket engine. The research was divided into two parts: the first part was devoted to obtaining the initiation of rotating detonation in fuel–oxygen mixture; the second was aimed at determination of the range of propagation stability as a function of chamber pressure, composition, and geometry. Additionally, thrust and specific impulse were determined in the latter stage. In the paper, only rich mixture is described, because using such a composition in rocket combustion chambers maximizes the specific impulse and thrust. In the experiments, two kinds of geometry were examined: cylindrical and cylindrical-conic, the latter can be simulated by a simple aerospike nozzle. Methane, ethane, and propane were used as fuel. The pressure–time courses in the manifolds and in the chamber are presented. The thrust–time profile and detonation velocity calculated from measured pressure peaks are shown. To confirm the performance of a rocket engine with rotating detonation as a high energy gas generator, a model of a simple engine was designed, built, and tested. In the tests, the model of the engine was connected to the dump tank. This solution enables different environmental conditions from a range of flight from 16 km altitude to sea level to be simulated. The obtained specific impulse for pressure in the chamber of max. 1.2 bar and a small nozzle expansion ratio of about 3.5 was close to 1,500 m/s.
Usable, Secure, Private Search (2011)

Real-world applications commonly require untrusting parties to share sensitive information securely. This article describes a secure anonymous database search (SADS) system that provides exact keyword match capability. Using a new reroutable encryption and the ideas of Bloom filters and deterministic encryption, SADS lets multiple parties efficiently execute exact-match queries over distributed encrypted databases in a controlled manner. This article further considers a more general search setting allowing similarity searches, going beyond existing work that considers similarity in terms of error tolerance and Hamming distance. This article presents a general framework, built on the cryptographic and privacy-preserving guarantees of the SADS primitive, for engineering usable private secure search systems.
AC133<sup>+</sup>G<sub>0</sub>Cells from Cord Blood Show a High Incidence of Long‐Term Culture–Initiating Cells and a Capacity for More Than 100 Million‐Fold Amplification of Colony‐Forming Cells In Vitro (2004)

AC133+ cells may provide an alternative to CD34+ cells as a target for cell expansion and gene therapy protocols. We examined the differences in proliferative potential between cord blood selected for AC133 or CD34 in serum-free, stroma cell-free culture for up to 30 weeks. Because most hemopoietic stem cells reside within the G0/G1 phase of the cell cycle, we combined enrichment according to AC133 or CD34 expression with G0 position in the cell cycle to identify populations enriched for putative stem cells. Our results show that AC133+ G0 cells demonstrated a long-term culture-initiating cell incidence of 1 in 4.2 cells, had a colony-forming cell incidence of 1 in 2.8 cells, were capable of producing 660 million-fold expansion of nucleated cells and 120 million-fold expansion of colony-forming units-granulocyte-macrophage over a period of 30 weeks, and were consistently superior to CD34+ G0 cells according to these parameters. Furthermore, we have shown that AC133+CD34- cells have the ability to generate CD34+ cells in culture, which suggests that at least some AC133+ cells are ancestral to CD34+ cells. We conclude that AC133 isolation provides a better means of selection for primitive hemopoietic cells than CD34 and that, in combination with isolation according to G0 phase of the cell cycle, AC133 isolation identifies a highly enriched population of putative stem cells.
TWO-PHASE FLOW, BOILING AND CONDENSATION IN CONVENTIONAL AND MINIATURE SYSTEMS (2010)

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A Novel Semi-biosynthetic Route for Artemisinin Production Using Engineered Substrate-Promiscuous P450 <sub>BM3</sub> (2009)

Production of fine chemicals from heterologous pathways in microbial hosts is frequently hindered by insufficient knowledge of the native metabolic pathway and its cognate enzymes; often the pathway is unresolved, and the enzymes lack detailed characterization. An alternative paradigm to using native pathways is de novo pathway design using well-characterized, substrate-promiscuous enzymes. We demonstrate this concept using P450(BM3) from Bacillus megaterium. Using a computer model, we illustrate how key P450(BM3) active site mutations enable binding of the non-native substrate amorphadiene. Incorporating these mutations into P450(BM3) enabled the selective oxidation of amorphadiene artemisinic-11S,12-epoxide, at titers of 250 mg L(-1) in E. coli. We also demonstrate high-yielding, selective transformations to dihydroartemisinic acid, the immediate precursor to the high-value antimalarial drug artemisinin.
Survey on inpatient medical experience at five public hospitals in Henan (2015)

Objective
To learn the medical experience of inpatients at public hospitals in Henan and the influencing factors.


Methods
Inpatient experience questionnaire(IPEQ)was customized for a random sampling of 500 inpatients at five tertiary public hospitals in Henan.


Results
Overall satisfaction of inpatients experience scored 8.48, of which the satisfaction for technical competence was the highest(4.19)and that for emotional support the lowest(3.31). The correlation analysis revealed that the doctors' technical competence score the highest correlation with the experiences(0.652).


Conclusion
Overall satisfaction of inpatients experience at public hospitals in Henan was found high in general, but humanistic care and service flow at the hospitals require further improvement.


Key words: 
Henan province; Public hospitals; Inpatients; Experiences; Satisfaction
Evidence for tissue‐specific forms of glutaminyl cyclase (1999)

Glutaminyl cyclase (QC) is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. An examination of the bovine tissue distribution of QC immunoreactivity, enzyme activity, and mRNA confirmed that QC was abundant in brain and pituitary by all three measures. However, enzymatic activity was considerably more widespread than either immunoreactivity or mRNA, suggesting multiple enzyme forms. Partially purified QC from bovine spleen differed significantly from the known bovine pituitary QC in physical and catalytic properties. We propose that this form of glutaminyl cyclase plays a role in the posttranslational processing of constitutively secreted pGlu-containing proteins.
Potential gains in life expectancy of Kuwaiti nationals through partial and complete elimination of infectious and parasitic disease mortality. (1988)

This study attempts to estimate potential gains in life expectancy in 1984 through partial and complete elimination of infectious and parasitic diseases among Kuwaiti nationals. For deriving these estimates, a multiple decrement life table approach was applied. The results of the study show that complete elimination of infectious and parasitic disease mortality would further add 0.45 years to the life expectancy at birth of Kuwaiti males and 0.54 yr to that of Kuwaiti females. A partial reduction by 50% would result in gains of just one-half of the years gained through complete elimination of these causes. Also, as partial reduction is increased from 10% to 90%, the gains in life expectancy at birth would go up from 0.04 yr to 0.40 yr in the case of Kuwaiti males and 0.06 to 0.48 yr for Kuwaiti females.
Semiconductor Superlattices-A New Material For Research and Applications (1981)

Progress in crystal growth techniques has made feasible the realization of periodic semiconductor structures composed of ultrathin layers. Essentially two types of such "man-made" superlattices may be distinguished: (i) Compositional superlattices, consisting of a periodic sequence of two different semiconductor materials with lattice matching (GaAs-AlxGa1-xAs, for example). A superlattice potential is induced in the conduction and the valence band by the periodic variation of the energy gap in the direction of crystal growth. (ii) Doping superlattices, a sequence of n- and p-doped layers, possibly with undoped layers in between ("nipi-structures"), grown in an otherwise homogeneous semiconductor bulk. The periodic potential in nipi-crystals is due to the electrostatic potential of fixed ionized impurities, which may be partly compensated by mobile electrons and holes confined to the n- and p-layers, respectively.
TNF-alpha reverses the disease-exacerbating effect of subcutaneous immunization against murine cutaneous leishmaniasis. (1991)

Earlier studies have demonstrated that mice injected subcutaneously or intramuscularly with leishmanial antigens develop significantly exacerbated disease compared with unimmunized controls when challenged with the cutaneous protozoan parasites Leishmania major. We report here that this disease enhancement can be prevented, and protective immunity induced, by the incorporation of recombinant tumour necrosis factor (TNF-alpha) in the immunizing inoculum. This effect of TNF-alpha is dose-dependent and is not evident when TNF-alpha and the antigens are injected into separate sites. Furthermore, TNF-alpha injected together with p183, a peptide known to preferentially stimulate Th2 cells and disease exacerbation in H-2d mice, activates spleen and lymph node cells secreting more interferon-gamma (IFN-gamma) and less interleukin-4 (IL-4) and induces a modest but significant degree of resistance against L. major infection in highly susceptible BALB/c mice.
Synthesis of PtRu Nanoparticles from the Hydrosilylation Reaction and Application as Catalyst for Direct Methanol Fuel Cell (2005)

Nanosized Pt, PtRu, and Ru particles were prepared by a novel process, the hydrosilylation reaction. The hydrosilylation reaction is an effective method of preparation not only for Pt particles but also for other metal colloids, such as Ru. Vulcan XC-72 was selected as catalyst support for Pt, PtRu, and Ru colloids, and TEM investigations showed nanoscale particles and narrow size distribution for both supported and unsupported metals. All Pt and Pt-rich catalysts showed the X-ray diffraction pattern of a face-centered cubic (fcc) crystal structure, whereas the Ru and Ru-rich alloys were more typical of a hexagonal close-packed (hcp) structure. As evidenced by XPS, most Pt and Ru atoms in the nanoparticles were zerovalent, except a trace of oxidation-state metals. The electrooxidation of liquid methanol on these catalysts was investigated at room temperature by cyclic voltammetry and chronoamperometry. The results concluded that some alloy catalysts showed higher catalytic activities and better CO tolerance than the Pt-only catalyst; Pt56Ru44/C have displayed the best electrocatalytic performance among all carbon-supported catalysts.
Spatial Planning: A Configuration Space Approach (1983)

This paper presents algorithms for computing constraints on the position of an object due to the presence of ther objects. This problem arises in applications that require choosing how to arrange or how to move objects without collisions. The approach presented here is based on characterizing the position and orientation of an object as a single point in a configuration space, in which each coordinate represents a degree of freedom in the position or orientation of the object. The configurations forbidden to this object, due to the presence of other objects, can then be characterized as regions in the configuration space, called configuration space obstacles. The paper presents algorithms for computing these configuration space obstacles when the objects are polygons or polyhedra.
Efficient Lipase-Catalyzed Enantioselective Desymmetrization of Prochiral 2,2-Disubstituted 1,3-Propanediols and Meso 1,2-Diols Using 1-Ethoxyvinyl 2-Furoate (2001)

An efficient lipase-catalyzed desymmetrization of prochiral 2,2-disubstituted 1,3-propanediols was developed using 1-ethoxyvinyl 2-furoate 1b, for which the well-known method using vinyl or isopropenyl acetate has had limited success due to low reactivity and easy racemization of the products through acyl group migration. The reagent 1b is highly reactive and converts various prochiral 1,3-diols to the monoesters having a chiral quaternary carbon center with 82-99% ee. These products were stable against racemization under acidic conditions, and their furoyl groups were compatible with oxidative conditions. Prolonging the reaction time led to the kinetic resolution of the monoesters resulting in an increase of their optical purity. The similar desymmetrization of meso cis-1,2-cycloalkanediols gave the monoesters with 82-97% ee without racemization.
NMR analysis of human salivary mucin (MUC7) derived <i>O</i>‐linked model glycopeptides: comparison of structural features and carbohydrate–peptide interactions (1999)

Two series of glycopeptides with mono- and disaccharides, [GalNAc and Galbeta (1-3)GalNAc] O-linked to serine and threonine at one, two or three contiguous sites were synthesized and characterized by 1H NMR. The conformational effects governed by O-glycosylation were studied and compared with the corresponding non-glycosylated counterparts using NMR, CD and molecular modelling. These model peptides encompassing the aa sequence, PAPPSSSAPPE (series I) and APPETTAAPPT (series II) were essentially derived from a 23-aa tandem repeat sequence of low molecular weight human salivary mucin (MUC7). NOEs, chemical shift perturbations and temperature coefficients of amide protons in aqueous and nonaqueous media suggest that carbohydrate moiety in threonine glycosylated peptides (series II) is in close proximity to the peptide backbone. An intramolecular hydrogen bonding between the amide proton of GalNAc or Galbeta (1-3)GalNAc and the carbonyl oxygen of the O-linked threonine residue is found to be the key structure stabilizing element. The carbohydrates in serine glycosylated peptides (series I), on the other hand, lack such intramolecular hydrogen bonding and assume a more apical position, thus allowing more rotational freedom around the O-glycosidic bond. The effect of O-glycosylation on peptide backbone is clearly reflected from the observed overall differences in sequential NOEs and CD band intensities among the various glycosylated and non-glycosylated analogues. Delineation of solution structure of these (glyco)peptides by NMR and CD revealed largely a poly L-proline type II and/or random coil conformation for the peptide core. Typical peptide fragments of tandem repeat sequence of mucin (MUC7) showing profound glycosylation effects and distinct differences between serine and threonine glycosylation as observed in the present investigation could serve as template for further studies to understand the multifunctional role played by mucin glycoproteins.
Sensitivity analysis for the appraisal of hydrofractures in horizontal wells with borehole resistivity measurements (2013)

ABSTRACT We numerically evaluate the possibility of using borehole electromagnetic measurements to diagnose and quantify hydraulic fractures that have been artificially generated in a horizontal well. Hydrofractures are modeled as thin disks perpendicular to the well and filled with either sand-based or electrically conductive proppant. The study focuses on the effect of thickness and length (radius) of hydrofractures to assess their effects on specific configurations of borehole-resistivity instruments. Numerical results indicate that several measurements (e.g., those obtained with low- and high-frequency solenoids) could be used to assess the thickness of a fracture. However, only low-frequency measurements performed with electrodes and large-spacing between transmitter and receivers (18 m) exhibit the necessary sensitivity to reliably and accurately estimate the length of long hydrofractures (up to 150 m) in open-hole wells. In the case of steel-cased wells, the casing acts as a long electrode, whereby conventional low-frequency short-spaced, through-casing measurements are suitable for the accurate diagnosis of long hydrofractures (up to 150 m in length).
A new kinetic approach for studying phospholipase C (Clostridium perfringens .alpha. toxin) activity on phospholipid monolayers (1988)

The enzymatic activity of purified phospholipase C (alpha toxin) from Clostridium perfringens was investigated with various phospholipid monolayers. A two-step reaction was used. Enzymatic hydrolysis of insoluble lecithin films by phospholipase C, generating 1,2-diacylglycerol and water-soluble phosphocholine, was coupled with the action of pancreatic lipase in order to give rise to fatty acid and 2-monoacylglycerol, which are rapidly desorbed from the interface. With this new procedure, it is possible to obtain continuous and accurate kinetic measurements of the phospholipase C catalyzed reaction with phospholipid monolayers as the substrate. It is thus possible to avoid the use of radiolabeled substrates as necessary in previous studies, and the difficulties caused by diacylglycerol accumulation in the lipid film are minimized. No hydrolysis was detected when either phosphatidylethanolamine, phosphatidylserine, or phosphatidylglycerol films were used as substrates. By means of a film transfer technique, Ca2+ and Zn2+ ions were found to play a specific and critical role. The present study demonstrates clearly for the first time that Ca2+ is essential for enzyme binding to lipid films, whereas Zn2+ is specifically involved in the catalytic hydrolysis of the substrate.
Pathobiology of the stratum corneum. (1993)

The epidermis is a dynamic system whose metabolic activity is regulated in large part by the integrity of the permeability barrier. This barrier resides in the stratum corneum and comprises a unique 2-compartment system of structural protein-enriched corneocytes embedded in a lipid-enriched intercellular matrix. Lipid extraction or metabolic imbalances, such as essential fatty acid deficiency, produce barrier abnormalities that in turn result in epidermal hyperproliferation, scaling, and inflammation. When the barrier remains intact, lipid imbalances, such as an abnormal cholesterol sulfate:cholesterol ratio in recessive X-linked ichthyosis, can lead to abnormal corneocyte adhesion (visible scale). Both cellular and intercellular proteins also participate in normal desquamation, and protein abnormalities may provoke abnormal scaling (such as filaggrin in ichthyosis vulgaris). Thus, perturbations of the stratum corneum may be the catalyst for a number of skin diseases, rather than the end result of processes that are initiated in subjacent skin layers.
Continuous scanning from picoseconds to microseconds in time resolved linear and nonlinear spectroscopy (2004)

We describe a setup of two synchronized femtosecond 1 kHz oscillator/regenerative amplifier laser systems that allows to continuously delay the amplified pulses up to 50 μs, with a jitter of ⩽2 ps. It can be realized by facile modification and combination of equipment that already exists in many femtosecond laser laboratories. It opens up a variety of experimental possibilities, particularly for the investigation of systems that display dynamics over a wide range of time scales, such as peptides and proteins. As an example we discuss the application of this setup for time resolved IR spectroscopy.