gebhart/patrepeval huggingface dataset:
Receptors on lymphocytes for endogenous splenic glycosaminoglycans. (1989)

Previous studies have shown that lymphocytes carry cell surface receptors for sulphated polysaccharides (SPS), and SPS recognition may play a role in lymphocyte migration and positioning in vivo. This paper describes attempts to isolate and characterize the endogenous glycosaminoglycans (GAGs) of murine spleen and determine whether splenic lymphocytes carry cell surface receptors for these GAGs. A procedure was devised for isolating GAGs from murine spleen in good yield and high purity and the GAG preparation was then radiolabelled for subsequent binding studies. It was found that the splenic GAGs bound to murine splenocytes in a saturable, rapid and reversible manner with only a small subpopulation of the splenic GAG preparation being involved in binding. This reactive species was chondroitinase ABC-resistant and nitrous acid-sensitive, indicative of a heparan sulphate/heparin-like molecule. Furthermore, using immunofluorescent flow cytometry studies it was demonstrated that the majority of spleen cells have receptors for these GAGs. Subsequent ion-exchange fractionation and SDS-PAGE analysis of chondroitinase ABC-resistant GAGs confirmed that the splenic GAG recognized by splenocytes was a heparan sulphate/heparin molecule of approximately 20,000 MW with a binding affinity to splenocytes of approximately 5 X 10(-8) M. Additional binding inhibition studies indicated two possible binding sites for splenic GAGs on the splenocyte surface, one being fully inhibited by a range of SPS such as heparin (both coagulant and anticoagulant forms), pentosan sulphate, fucoidan, dextran sulphate, lambda- and iota-carrageenan, and the second being partially inhibited by kappa-carrageenan. The possible relevance of these heparan sulphate/heparin receptors on splenocytes to lymphocyte positioning in vivo is discussed.
Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for stress proteins in oncogenic transformation. (1994)

The molecular mechanisms by which oncogenic tyrosine kinases induce cellular transformation are unclear. Herbimycin A, geldanamycin, and certain other benzoquinone ansamycins display an unusual capacity to revert tyrosine kinase-induced oncogenic transformation. As an approach to the study of v-src-mediated transformation, we examined ansamycin action in transformed cells and found that drug-induced reversion could be achieved without direct inhibition of src phosphorylating activity. To identify mechanisms other than kinase inhibition for drug-mediated reversion, we prepared a solid phase-immobilized geldanamycin derivative and affinity precipitated the molecular targets with which the drug interacted. In a range of cell lines, immobilized geldanamycin bound elements of a major class of heat shock protein (HSP90) in a stable and pharmacologically specific manner. Consistent with these binding data, we found that soluble geldanamycin and herbimycin A inhibited specifically the formation of a previously described src-HSP90 heteroprotein complex. A related benzoquinone ansamycin that failed to revert transformed cells did not inhibit the formation of this complex. These results demonstrate that HSP participation in multimolecular complex formation is required for src-mediated transformation and can provide a target for drug modulation.
Instrumentation for Chemical Cytometry (2000)

Capillary electrophoresis is ideally suited to chemical analysis of individual cells. Small mammalian somatic cells (approximately 15 microns in diameter) can be analyzed by injecting the intact cell into a capillary, lysing the cell, separating and detecting the cellular components, and reconditioning the capillary prior to the next injection. In this paper, we report on technical improvements to single-cell analysis. We designed an inexpensive multipurpose single-cell injector that facilitates the following: (i) monitoring of injection, (ii) reproducible pressure- or electrokinetic-driven injection of the cell, (iii) complete cell lysis by SDS within 30 s of injection, and (iv) pressure-driven capillary reconditioning. Furthermore, we report on the analysis of glycosylation and glycolysis in single human carcinoma cells (HT29 cell line). The reliability and quality of the analysis is confirmed by comparing electropherograms from single cells and those from purified cell extracts.
Heme coordination and structure of the catalytic site in nitric oxide synthase. (1993)

Nitric oxide (NO), recently found to play many physiological roles, is generated by the catalysis of L-arginine and O2 to L-citrulline and NO by nitric oxide synthases (NOSs). Resonance Raman spectra from the heme of resting, reduced, and CO-bound forms of rat brain NOS firmly establish that the enzyme belongs to the P-450 class of enzymes. The electron density marker line (V4) in the Raman spectrum of ligand-free ferrous NOS has a low frequency (1347 cm-1), indicating a thiolate axial ligand on the heme. The assignment of a thiolate axial ligand is confirmed in the CO-bound form of the enzyme by the frequency of the Fe-C-O bending mode at 562 cm-1. The heme is resting NOS is five-coordinate high spin and thereby differs from the resting state of most substrate-free P-450s, which are predominantly six-coordinate low spin. The frequency of the Fe-CO stretching mode in the CO-bound enzyme at 491 cm-1, identified by isotope substitution, in higher than that in substrate-free P-450s. Thus, in the ferric and the CO-bound forms of the enzyme, the sixth-ligand binding site on the heme is restricted by steric or hydrophobic interactions. In addition, the Fe-CO stretching mode is broad (30 cm-1) and may be resolved into two overlapping lines of equal intensity, indicating that the heme domains can adopt two distinct conformations.
Clinical Characteristics of Somatic Mutations in Chinese Patients With Aldosterone-Producing Adenoma (2015)

Recent studies have shown that somatic mutations in the KCNJ5, ATP1A1, ATP2B3, and CACNA1D genes are associated with the pathogenesis of aldosterone-producing adenoma. Clinical profile and biochemical characteristics of the mutations in Chinese patients with aldosterone-producing adenoma remain unclear. In this study, we performed DNA sequencing in 168 Chinese patients with aldosterone-producing adenoma and found 129 somatic mutations in KCNJ5, 4 in ATP1A1, 1 in ATP2B3, and 1 in CACNA1D. KCNJ5 mutations were more prevalent in female patients and were associated with larger adenomas, higher aldosterone excretion, and lower minimal serum K(+) concentration. More interestingly, we identified a novel somatic KCNJ5 mutation (c.445-446insGAA, p.T148-T149insR) that could enhance CYP11B2 mRNA upregulation and aldosterone release. This mutation could also cause membrane depolarization and intercellular Ca(2+) increase. In conclusion, somatic KCNJ5 mutations are conspicuously more popular than mutations of other genes in aldosterone-producing adenomas of Chinese patients. The T148-T149insR mutation in KCNJ5 may influence K(+) channel selectivity and autonomous aldosterone production.
High-voltage DC transmission: a power electronics workhorse (1996)

Thyristor-based HVDC converter technology is used for highly reliable power transfer across natural or national boundaries or between AC systems designed for different frequencies or incompatible frequency controls. The author discusses the benefits of HVDC transmission and describes HVDC transmission system configurations. The author then describes the use of thyristors in HVDC converters.
Tensor video coding (2013)

In this paper, we exploit the intrinsic tensor-nature of video and propose a Tensor Video Coding (TVC) framework that is based on tensor decomposition. We develop a Progressive Canonical-decomposition Parallel-factor (PCP) framework that is tailored for video representation and coding. Our simulation results show that the proposed TVC approach outperforms state-of-the-art video coding methods that do not rely on motion estimation or compensation.
Novel Receiver Sensor for Visible Light Communications in Automotive Applications (2015)

This paper presents an easy-to-use sensor aimed for traffic safety applications using visible light communications. A central problem in this area is the design of a suitable sensor able to enhance the conditioning of the signal and to avoid disturbances due to the environmental conditions. The visible light communication sensor proposed in this paper addresses these issues and enables a robust communication for short to medium distances. The presentation is focused on hardware aspects and low-level coding techniques. The experimental validation of the proposed sensor has been conducted by analyzing communication performances between a commercial traffic light and the sensor, for distances up to 50 m. The measurements exhibit bit error ratio lower than $10^{-7}$ in an outdoor configuration , using two well-known codes (Manchester and Miller) without any error-correcting codes or complex signal processing. The prototype is aimed for automotive applications but other outdoor configurations can also be addressed by slight changes in the system design.
A Hybrid Photonic Integrated Wavelength Converter on a Silicon-on-Insulator Substrate (2012)

We present fabrication and testing of a wavelength converter integrated on a silicon-on-insulator substrate. The chip employs a hybrid integrated SOA and delay-interferometers integrated on 4μm SOI. We demonstrate 40Gb/s error-free performance.
Generalization error bounds in semi-supervised classification under the cluster assumption (2006)

We consider semi-supervised classification when part of the available data is unlabeled. These unlabeled data can be useful for the classification problem when we make an assumption relating the behavior of the regression function to that of the marginal distribution. Seeger (2000) proposed the well-known "cluster assumption" as a reasonable one. We propose a mathematical formulation of this assumption and a method based on density level sets estimation that takes advantage of it to achieve fast rates of convergence both in the number of unlabeled examples and the number of labeled examples.
Monoclonal antibodies in biology and biotechnology:theoretical and practical aspects (1990)

Monoclonal antibodies are one of the most exciting developments in biotechnology in recent years; this book provides a comprehensive description of principles, methodologies and applications of this powerful technology to modern science and industry. The immune system of the body, in particular the role of antibodies, is described; and details provided on the isolation of antibody-producing cells, how hybridomas are formed and maintained in culture such that the required antibody can be produced to a very high degree of purity. The material is presented in such a way that students, researchers and managers in research and development will be able to make choices as to which are the most suitable techniques for their particular requirements. The text is comprehensively referenced and, in short, the book is a necessary resource for all those who have the need to be involved in the production of monoclonal antibodies from hybridoma cells in culture.
A simple turbulence model for stably stratified wall‐bounded flows (2014)

Abstract In this study, we present a simple zero‐equation (algebraic) turbulence closure scheme as well as the standard k ‐ ϵ model for stably stratified wall‐bounded flows. We do this by proposing a parameterization for the turbulent Prandtl number ( Pr t ) for stably stratified flows under the influence of a smooth solid wall. The turbulent Prandtl number is the linking bridge between the turbulent momentum and scalar fluxes in the context of Reynolds‐averaged Navier‐Stokes (RANS) simulations. Therefore, it is important to use appropriate parameterizations for Pr t in order to define the right level of momentum and scalar mixing in stably stratified flows. To date, most of the widely used parameterizations for Pr t in stably stratified flows are based on data obtained from homogeneous shear flows experiments and/or direct numerical simulations (i.e., statistics are invariant under translations) and are usually formulated as functions of the gradient Richardson number ( Ri g ). The effect of the wall boundary is completely neglected. We introduce a modified parameterization for Pr t that takes into account the inhomogeneity caused by the wall coupled with the effects of density stratification. We evaluate the performance of the modified Pr t by using a zero‐equation turbulence model for the turbulent viscosity that was proposed by Munk and Anderson (1948) as well as the standard k ‐ ϵ model to simulate a one‐dimensional stably stratified channel flow. Comparison of the one‐dimensional simulation results with direct numerical simulation (DNS) of stably stratified channel flow results show remarkable agreement.
Metascape provides a biologist-oriented resource for the analysis of systems-level datasets (2019)

A critical component in the interpretation of systems-level studies is the inference of enriched biological pathways and protein complexes contained within OMICs datasets. Successful analysis requires the integration of a broad set of current biological databases and the application of a robust analytical pipeline to produce readily interpretable results. Metascape is a web-based portal designed to provide a comprehensive gene list annotation and analysis resource for experimental biologists. In terms of design features, Metascape combines functional enrichment, interactome analysis, gene annotation, and membership search to leverage over 40 independent knowledgebases within one integrated portal. Additionally, it facilitates comparative analyses of datasets across multiple independent and orthogonal experiments. Metascape provides a significantly simplified user experience through a one-click Express Analysis interface to generate interpretable outputs. Taken together, Metascape is an effective and efficient tool for experimental biologists to comprehensively analyze and interpret OMICs-based studies in the big data era.
On the Fixed-Point Accuracy Analysis of FFT Algorithms (2008)

In this paper, we investigate the effect of fixed-point arithmetics with limited precision for different fast Fourier transform (FFT) algorithms. A matrix representation of error propagation model is proposed to analyze the rounding effect. An analytic expression of overall quantization loss due to the arithmetic quantization errors is derived to compare the performance with decimation-in-time (DIT) and decimation-in-frequency (DIF) configurations. From the simulation results, the radix-2 DIT FFT algorithm has better accuracy in term of signal-to-quantization-noise ratio (SQNR). Based on the results, a simple criterion of wordlength optimization is proposed to yield comparable accuracy with fewer bit budget.
LENGTH SCALES IN THE STATIC AND DYNAMIC TORSION OF A CIRCULAR CYLINDRICAL MICRO-BAR (2001)

The static and dynamic torsion of a circular cylindrical micro-bar is analyzed within the couple stress theory The static shear stresses and natural frequencies are solved for the circular cylinder with couple stresses The maximum shear stress will eventually drop and the natural frequencies will increase with the cylinder radius close to the material length scale
Determination of Isosmolar Blood Anticoagulant Solutions by the Freezing-Point Method (1958)

Abstract Freezing-point depressions of several anticoagulants were determined from which concentrations isosmolar with the blood of any species may be computed. The method is not applicable to solutes of high molecular weight.
Effect of the Electric Field Generated by the Helix Dipole on Photoinduced Intramolecular Electron Transfer in Dichromophoric α-Helical Peptides (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTEffect of the Electric Field Generated by the Helix Dipole on Photoinduced Intramolecular Electron Transfer in Dichromophoric α-Helical PeptidesElena Galoppini and Marye Anne FoxView Author Information Department of Chemistry and Biochemistry University of Texas at Austin, Austin, Texas 78712 Cite this: J. Am. Chem. Soc. 1996, 118, 9, 2299–2300Publication Date (Web):March 6, 1996Publication History Received12 May 1995Published online6 March 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/ja951555ahttps://doi.org/10.1021/ja951555arapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views1415Altmetric-Citations191LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information SUBJECTS:Charge transfer,Electric fields,Molecular structure,Peptides and proteins,Solvents Get e-Alerts
The Application of Value Stream Mapping Based Lean Production System (2010)

Many enterprises in Chinese Mainland have deployed lean production in consideration of its great success inother countries, but many of them failed to realize their original targets because they began to deploy leanproduction from tool level and haven’t understood the actual value stream of their business, which is essential foran enterprise to implement lean production and keep improving. The paper proposes a value stream mappingbased lean production system for Chinese enterprises to help them deploy lean production systematically, whichcan make them have a overall look at total efficiency, tell them where they are, where they want to go and map aroute to get there. We hope the paper will help them eliminate roots of wastes, rearrange overall value streambetter and increase the competitive ability of Chinese enterprises.
Reaction of bis(2,2,2-trinitroethyl) formal with potassium cyanide (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReaction of bis(2,2,2-trinitroethyl) formal with potassium cyanideMortimer J. KamletCite this: J. Chem. Eng. Data 1969, 14, 1, 116–117Publication Date (Print):January 1, 1969Publication History Published online1 May 2002Published inissue 1 January 1969https://pubs.acs.org/doi/10.1021/je60040a038https://doi.org/10.1021/je60040a038research-articleACS PublicationsRequest reuse permissionsArticle Views33Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Towards Creating Public Key Authentication for IoT Blockchain (2019)

Besides confidentiality and privacy, trust is an important factor for any IoT system. When a sensor send data that is signed with its private key, the receiving nodes verify it using the public key of the sensor. Hence, it is understood that authenticating the public key of the system is part of creating trust within the system. Traditionally, trust is maintained using Public Key Infrastructure (PKI) where a centralized Certificate Authority (CA) is used for authenticating the public keys. However, a centralized system can result in single point of failure where CA can be compromised or can act maliciously. Decentralizing this system using blockchain and by automating the process of certificate authentication without the need for a central third party can overcome the abovementioned limitations. We identify the challenges in creating such a system and propose a generic framework for PKI in IoT infrastructure using blockchain that can provide the functions of a CA.
α-Synuclein Is Required for the Fibrillar Nature of Ubiquitinated Inclusions Induced by Proteasomal Inhibition in Primary Neurons (2004)

Proteasomal dysfunction may underlie certain neuro-degenerative conditions such as Parkinson disease. We have shown that pharmacological inhibition of the proteasome in cultured neuronal cells leads to apoptotic death and formation of cytoplasmic ubiquitinated inclusions. These inclusions stain for alpha-synuclein and assume a fibrillar structure, as assessed by thioflavine S staining, and therefore resemble Lewy bodies. alpha-Synuclein is thought to be a central component of Lewy bodies. Whether alpha-synuclein is required for inclusion formation or apoptotic death has not been formally assessed. The present study examines whether alpha-synuclein deficiency in neurons alters their sensitivity to proteasomal inhibition-induced apoptosis or inclusion formation. Cortical neurons derived from alpha-synuclein-null mice showed a similar sensitivity to death induced by the proteasomal inhibitor lactacystin compared with neurons derived from wild-type mice. Furthermore, the absence of alpha-synuclein did not influence the percentage of lactacystin-treated neurons harboring cytoplasmic ubiquitinated inclusions or alter the solubility of such inclusions. In contrast, however, ubiquitinated inclusions in alpha-synuclein-deficient neurons lacked amyloid-like fibrillization, as determined by thioflavine S staining. This indicates that although alpha-synuclein deficiency does not affect the formation of ubiquitinated inclusions, it does significantly alter their structure. The lack of effect on survival in alpha-synuclein knock-out cultures further suggests that the fibrillar nature of the inclusions does not contribute to neuronal degeneration in this model.
Monitoring of Power Station Plant within Latrobe Valley-based Power Stations (1980)

The monitoring of turbogenerators operated by the State Electricity Commission of Victoria (SECV) in the Latrobe Valley is described. The techniques discussed include performance and vibration monitoring and the use of automatic data processing is forecast.
A comparison study of succinct data structures for use in GWAS (2013)

In this work, we were concerned with comparing the performance benefits and disadvantages of using more densely packed genotype data representations in Genome Wide Associations Studies (GWAS). We implemented a 2-bit encoding for genotype data, and compared it against a more commonly used 3-bit encoding scheme. We also developed a C++ library, libgwaspp, which offers these data structures, and implementations of several common GWAS algorithms. In general, the 2-bit encoding consumes less memory, and is slightly more efficient in some algorithms than the 3-bit encoding.