gebhart/patrepeval huggingface dataset:
Broad-band single-patch circularly polarized microstrip antenna with dual capacitively coupled feeds (2001)

New design of a circular microstrip antenna with dual capacitively coupled feeds for broad-band circular polarization radiation is presented. The dual feeds are with a small top-loaded disk and are connected to a Wilkinson power divider with a 90/spl deg/ phase shift between its two output feedlines. The radiating circular patch, printed on a thin substrate, is supported by nonconducting posts on a conducting ground plane and is excited capacitively through the dual feeds. With a distance less than 10% times the center operating wavelength between the circular patch and the ground plane, the present proposed antenna can provide an impedance bandwidth (VSWR /spl les/2) of about 49% and a 3-dB axial-ratio bandwidth of about 35%. The antenna gain bandwidth, defined to be within 1-dB gain variation in the axial-ratio bandwidth, is as large as 28%, with the antenna gain level at about 7.0 dBi.
An advanced power processor for utility Grid Infrastructure (2010)

The United States National Grid Infrastructure, its transmission, its distribution, its security and its controls, rely on equipment dating back to the 19 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</sup> Century. In the last 17 years, it became painfully apparent that these legacy systems could not support the ever-increasing demand for energy, regardless of the energy generation source: wind, solar, geothermal, hydro or nuclear. These legacy infrastructure systems are aging rapidly and are directly responsible for increasing costs of operation, maintenance and directly contributing to down-stream failures, an unsustainable condition. With recent achievements, enhancements and maturity of power semiconductors devices and applicable systems by industry leaders, economically viable options to upgrade this critical infrastructure are market ready today. When properly planned, these scalable solid-state systems will seamlessly integrate into our national electrical energy infrastructure, support smart-grid technology, increase security, increase capacity, reduce environmental concerns and reduce operational and maintenance costs.
Droplet-based microfluidic system to form and separate multicellular spheroids using magnetic nanoparticles (2013)

The importance of creating a three-dimensional (3-D) multicellular spheroid has recently been gaining attention due to the limitations of monolayer cell culture to precisely mimic in vivo structure and cellular interactions. Due to this emerging interest, researchers have utilized new tools, such as microfluidic devices, that allow high-throughput and precise size control to produce multicellular spheroids. We have developed a droplet-based microfluidic system that can encapsulate both cells and magnetic nanoparticles within alginate beads to mimic the function of a multicellular tumor spheroid. Cells were entrapped within the alginate beads along with magnetic nanoparticles, and the beads of a relatively uniform size (diameters of 85% of the beads were 170-190 μm) were formed in the oil phase. These beads were passed through parallel streamlines of oil and culture medium, where the beads were magnetically transferred into the medium phase from the oil phase using an external magnetic force. This microfluidic chip eliminates additional steps for collecting the spheroids from the oil phase and transferring them to culture medium. Ultimately, the overall spheroid formation process can be achieved on a single microchip.
Transformation of <i>Arthrobacter</i> and studies on the transcription of the <i>Arthrobacter ermA</i> gene in <i>Streptomyces lividans</i> and <i>Escherichia coli</i> (1987)

We report the development of a plasmid-mediated transformation system for Arthrobacter sp. NRRLB3381, using the Streptomyces cloning vector pIJ702. Our procedure gives a transformation frequency of 10(3)/micrograms of plasmid DNA. In addition we have explored the expression of the Arthrobacter ermA gene in Streptomyces lividans and Escherichia coli, and shown that the ermA promoter is recognized in S. lividans not E. coli. The relationship between Arthrobacter, Streptomyces and E. coli promoters is discussed.
Cancer Vaccines. Any Future? (2011)

The idea that vaccination can be used to fight cancer is not new. Approximately 100 years ago, researchers attempted to stimulate a tumor-specific, therapeutic immune response to tumors by injecting patients with cells and extracts from their own tumors, or tumors of the same type from different individuals. During the last decade, great efforts have been made to develop immunotherapeutic approaches for the treatment of malignant diseases as alternatives to traditional chemo- and radiotherapy. A quintessential goal of immunotherapy in cancer is treatment with vaccines that elicit potent anti-tumor immune responses without side effects. In this article, we have attempted to review some of the most problematic issues facing the development of cancer vaccines. With the prospect of immunosuppression, an ill-designed cancer vaccine can be more harmful than a no-benefit therapy. We have noted that "immunoediting" and "immunodominance" are the premier setbacks in peptide-based vaccines and therefore it appears necessary not only to manipulate the activity of a vast number of principal components but also to finely tune their concentrations in time and space. In the face of all these quandaries, it is at least doubtful that any reliable anti-cancer vaccine strategy will emerge in the near future.
Microstructure of Nunas: Andean Popping Beans (Phaseolus Vulgaris L.) (1989)

Nuñas, popping beans (Phaseolus vulgaris L.), burst and expand when heated rapidly. Differences in seed microstructure between popping and conventional (non-popping) bean genotypes conceivably contribute to popping in ñuñas. However, the microstructural characteristics which contribute to the popping attribute and sites of expansion have not been identified. Seeds and excised cotyledons of unpopped and popped ñuñas were examined using scanning electron microscopy (SEM). Protoplasts of unpopped ñuñas were similar to protoplasts of conventional beans. Intercellular spaces of unpopped ñuñas were occuled by schizogenous cell walls. The occluded form of intercellular spaces differed distinctively from the open form in popped ñuñas and untreated conventional beans. The expansion of cotyledon mesophyll in popped ñuñas came primarily from expansion of cell walls and secondarily by expansion of the intercellular spaces. Cell wall thickness and dimensions of protoplasts were not changed during popping. Expansion of cell walls away from protoplasts created intracellular voids. SEM images indicated that starch granules (grains) in popped ñuñas were generally not altered by popping. Starch granules did not gelatinize or melt during popping as indicated by retention of birefringence. In contrast to popcorn (Zea mays L.), starch granules did not contribute to expansion of popped ñuña cotyledons
Effective Suicide Gene Therapy for Leukemia in a Model of Insertional Oncogenesis in Mice (2006)

The safety of gene therapy using hematopoietic stem cells may be increased by including a suicide gene in the therapeutic vector to eliminate adverse events like insertional oncogenesis while retaining the clinical benefits. We have developed a model of experimental insertional oncogenesis by transducing the murine factor-dependent leukemia cell line Ba/F3 with a bicistronic Moloney murine leukemia virus retroviral vector encoding a murine oncogene (cKit(D814V)) in addition to one of three suicide genes: Herpes simplex virus thymidine kinase (HSV-TK); SR39, an HSV-TK mutant with an increased affinity for the drug substrate Ganciclovir (GCV); or sc39, a splice-corrected version of SR39. Following intravenous challenge with transduced Ba/F3 clones and treatment with GCV, leukemia developed in mice given cells expressing HSV-TK, but not SR39 or sc39. In vitro GCV resistance was observed in heterogeneously transduced Ba/F3 pools at 2.5-14%, and single-nucleotide changes or partial loss of the suicide gene were identified as mechanisms of drug escape. However, GCV treatment resulted in 80-100% survival of mice challenged even with pools of partially resistant Ba/F3 cells expressing SR39 or sc39. Thus, in this model of vector-driven insertional oncogenesis, a suicide gene approach was effective for eliminating leukemia using modified HSV-TK variants with improved biological activity.
Expression and Effects of IL-33 and ST2 in Allergic Bronchial Asthma: IL-33 Induces Eotaxin Production in Lung Fibroblasts (2011)

IL-33 may contribute to the induction and maintenance of eosinophilic inflammation in the airways by acting on lung fibroblasts. IL-33 and ST2 may play important roles in allergic bronchial asthma.
TNF- <i>alpha</i> production by eosinophils in upper airways inflammation (nasal polyposis). (1994)

TNF-alpha is a cytokine with a wide spectrum of proinflammatory activities. Nasal polyps (NP), which occur in association with allergic rhinitis and asthma, are characterized by a marked infiltration of activated eosinophils, epithelial damage, and varying degrees of stromal fibrosis. By using Southern blot analysis after a reverse transcription-PCR, we detected a signal specific for TNF-alpha mRNA in five of seven NP samples, but not in control nasal mucosal samples. With in situ hybridization, we detected cells expressing mRNA specific for TNF-alpha in eight of thirteen NP samples, but not in four control samples. Counterstaining with chromotrope 2R demonstrated that virtually all cells expressing TNF-alpha message were eosinophils. The ratio of eosinophils expressing TNF-alpha to the total number of eosinophils varied greatly among tissues; as an average, we observed 24% TNF-alpha-positive eosinophils. Using a mAb against human TNF-alpha, we demonstrated TNF-alpha localization in 12 NP tissues (48.8 +/- 16.5 positive cells/mm2) but not in three control samples. Morphologically, cells localizing TNF-alpha were both mononucleated and polynucleated with only a small number of eosinophils, as determined by aniline blue counterstaining. Studies of purified blood eosinophils from a patient with hypereosinophilic syndrome and from four healthy donors indicated that TNF-alpha is produced, but rapidly secreted, so that TNF-alpha mRNA-positive cells contain little detectable protein. Furthermore, cells that retain detectable TNF-alpha may not contain sufficient TNF-alpha mRNA to be detected by using the probe developed for our studies. Together, these findings identify a novel mechanism by which eosinophils may contribute to mucosal inflammation and provide an approach to future investigation.
Molecular and cellular mechanisms of pharmacoresistance in epilepsy (2005)

Epilepsy is a common and devastating neurological disorder. In many patients with epilepsy, seizures are well-controlled with currently available anti-epileptic drugs (AEDs), but a substantial (∼30%) proportion of patients continue to have seizures despite carefully optimized drug treatment. Two concepts have been put forward to explain the development of pharmacoresistance. The transporter hypothesis contends that the expression or function of multidrug transporters in the brain is augmented, leading to impaired access of AEDs to CNS targets. The target hypothesis holds that epilepsy-related changes in the properties of the drug targets themselves may result in reduced drug sensitivity. Recent studies have started to dissect the molecular underpinnings of both transporter- and target-mediated mechanisms of pharmacoresistance in human and experimental epilepsy. An emerging understanding of these underlying molecular and cellular mechanisms is likely to provide important impetus for the development of new pharmacological treatment strategies.
Genome-Wide Linkage Analysis Assessing Parent-of-Origin Effects in the Inheritance of Type 2 Diabetes and BMI in Pima Indians (2001)

We examined the hypothesis that imprinted genes may affect the propensity to type 2 diabetes and obesity in Pima Indians. Multipoint variance component methods were used to assess linkage of BMI (kg/m(2)) and age-adjusted diabetes to loci derived from either father (LOD(FA)) or mother (LOD(MO)) in a genome-wide scan. Tentative evidence of loci where imprinted genes might be acting was found for diabetes with maternally derived alleles on chromosomes 5 (LOD(MO) = 1.5) and 14 (LOD(MO) = 1.6). Evidence of linkage of BMI to maternally derived alleles was found on chromosome 5 (LOD(MO) = 1.7) and to paternally derived alleles on chromosome 10p (LOD(FA) = 1.7). Additional analyses of sibling pairs who were affected by diabetes and younger than 25 years of age showed an increase of sharing of maternally derived alleles on chromosome 6 (LOD(MO) = 3.0). We also examined sites of a priori interest where action of imprinted genes has been proposed in diabetes or obesity. We found no evidence of parent-specific linkage (of either diabetes or BMI) on chromosome 11p, a region that contains several imprinted genes, but observed weak evidence of linkage of diabetes to paternally derived alleles (LOD(FA) = 0.9) in the region of chromosome 6q, believed to contain an exclusively paternally expressed gene or genes that cause transient neonatal diabetes mellitus. In conclusion, we determined regions of interest on chromosomes 5, 6, and 10 where imprinted genes might be affecting the risk of type 2 diabetes or obesity in Pima Indians.
Aero-Assisted Orbital Transfer Vehicle (AOTV) (1987)

The AOTV will make use of the atmosphere to provide braking on return from a planetary mission or geosynchronous orbit. The minimum altitude for aerobraking is typically 255,000 ft at the equator. Time of the braking maneuver is typically 480 sec from 400,000 ft to 255,000 ft and back out - about 8 min. The problem is to design a control system that will be able to handle density irregularities such as those that have shown up in shuttle data near 280,000 ft. To obtain data, one has to use model-produced statistics or information obtained during the atmospheric transit time. The Global Reference Atmosphere Model (GRAM) appears to bracket the shuttle data, but it is not clear that the statistics are correct. The model-data exhibits strong density shears over small step size that are probably an artifact.
Studies on Quinone-Thioethers. I. Mechanism of Formation and Properties of Thiodione<sup>*</sup> (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Quinone-Thioethers. I. Mechanism of Formation and Properties of Thiodione*Walter J. Nickerson, Giuseppe Falcone, and George StraussCite this: Biochemistry 1963, 2, 3, 537–543Publication Date (Print):May 1, 1963Publication History Published online1 May 2002Published inissue 1 May 1963https://doi.org/10.1021/bi00903a025RIGHTS & PERMISSIONSArticle Views376Altmetric-Citations117LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alerts Get e-Alerts
Testing the disaster recovery plan (1995)

Organizations need to have undertaken an analysis of business risk and to have formulated a recovery plan. However, only a small number of businesses have made any attempt to minimize their risks and even fewer have a recovery plan which has been tested. Most are a token gesture to auditors. An out‐of‐date, untested plan can often be more dangerous than not having a plan at all as it lulls the organization into a false sense of security. But how do you test plans? If the strategy is to use a computer‐processing facility in another place – perhaps a commercial hot and cold site – the costs of regular testing can be high and the disruption to the business great. In tight economic times, senior management is too often prepared to gamble with the organization′s future. Develops a testing methodology, based on many years of designing plans and hands‐on testing, which reduces costs by breaking down the test procedure into components, modules and full tests.
Decreased Urinary Sodium-to-urinary Creatinine Ratio Identifies Sodium Depletion in Pediatric Acute Gastroenteritis (2015)

The majority of AG patients was found to have NNaD as determined by uNa/uCr and FENa. Calculation of uNa/uCr may be useful for diagnosing NNaD in AG.
Interleukin 10, a novel B cell stimulatory factor: unresponsiveness of X chromosome-linked immunodeficiency B cells. (1990)

Highly purified, small dense splenic B cells from unstimulated mice showed increased expression of class II major histocompatibility complex (MHC) antigens and enhanced viability when cultured with affinity-purified recombinant interleukin 10 (rIL-10), compared with B cells cultured in medium alone. These responses were blocked by a monoclonal antibody (mAb) specific for IL-10, but not by an isotype-matched control antibody. IL-10 did not upregulate the expression of Fc epsilon receptors (CD23) or class I MHC antigens on small dense B cells or induce their replication as monitored by [3H]thymidine incorporation. While these B cell-stimulatory properties of IL-10 are also mediated by IL-4, the two cytokines appear to act independently in these assays; anti-IL-10 antibodies blocked IL-10 but not IL-4-mediated B cell viability enhancement, and vice versa. Similarly, since IL-4 upregulates CD23 on small dense B cells, the inability of IL-10 to do so argues against its acting via endogenously generated IL-4. Finally, IL-10 did not upregulate class II MHC antigens on B cells from X chromosome-linked immunodeficiency (XID) mice, while the same cells showed normal upregulation of class II antigens in response to IL-4. This report also extends our understanding of the relationship between IL-10 and the highly homologous Epstein-Barr virus (EBV)-encoded Bam HI fragment C rightward reading frame no. 1 (BCRFI) protein. It has previously been shown that BCRFI protein exhibits the cytokine synthesis inhibitory activity of IL-10. This report indicates that BCRFI protein also enhances in vitro B cell viability, but does not upregulate class II MHC antigens on B cells. One explanation for these data is that IL-10 contains at least two functional epitopes, only one of which has been conserved by EBV.
Direct Formation of Thin Single Crystals of Organic Semiconductors onto a Substrate (2007)

We have developed a novel and convenient method of crystal growth in a liquid phase. This method produces directly onto a substrate well-defined polygon organic thin crystals with uniform thickness. The thin crystals are found to be single crystals of high quality. We show various illustrations including thiophene/phenylene co-oligomers and an oligophenylene. The organic crystal transistors based on these crystals showed good device performance. These thin single crystals are expected to be suitably applied to electronic devices.
In Vitro Activity of Human Immunodeficiency Virus Protease Inhibitors against<i>Pneumocystis carinii</i> (2000)

Since 1996, the introduction of protease inhibitors (PIs) has led to a dramatic decrease of human immunodeficiency virus-related Pneumocystis carinii pneumonia. This effect is clearly due, in large part, to the induction of immune reconstitution by highly active antiretroviral therapy (HAART). However, it is conceivable that PIs had other beneficial effects, including direct activity against Pneumocystis. In this study, the occurrence of specific aspartyl proteases in Pneumocystis is described. These protease targets seemed to be affected in vitro by antiretroviral PIs. These data suggest intriguing implications for the possible antipneumocystis benefit of receiving indinavir, ritonavir, nelfinavir, or saquinavir during HAART.
RIL-Contour: a Medical Imaging Dataset Annotation Tool for and with Deep Learning (2019)

Deep-learning algorithms typically fall within the domain of supervised artificial intelligence and are designed to "learn" from annotated data. Deep-learning models require large, diverse training datasets for optimal model convergence. The effort to curate these datasets is widely regarded as a barrier to the development of deep-learning systems. We developed RIL-Contour to accelerate medical image annotation for and with deep-learning. A major goal driving the development of the software was to create an environment which enables clinically oriented users to utilize deep-learning models to rapidly annotate medical imaging. RIL-Contour supports using fully automated deep-learning methods, semi-automated methods, and manual methods to annotate medical imaging with voxel and/or text annotations. To reduce annotation error, RIL-Contour promotes the standardization of image annotations across a dataset. RIL-Contour accelerates medical imaging annotation through the process of annotation by iterative deep learning (AID). The underlying concept of AID is to iteratively annotate, train, and utilize deep-learning models during the process of dataset annotation and model development. To enable this, RIL-Contour supports workflows in which multiple-image analysts annotate medical images, radiologists approve the annotations, and data scientists utilize these annotations to train deep-learning models. To automate the feedback loop between data scientists and image analysts, RIL-Contour provides mechanisms to enable data scientists to push deep newly trained deep-learning models to other users of the software. RIL-Contour and the AID methodology accelerate dataset annotation and model development by facilitating rapid collaboration between analysts, radiologists, and engineers.
Colloidal Properties of Aqueous Suspensions of Acid-Treated, Multi-Walled Carbon Nanotubes (2008)

Grafting oxygen-containing functional groups onto carbon nanotubes (CNTs) by acid treatment improves their dispersion in aqueous solutions, but there is a lack of quantitative information on the colloidal properties of oxidized CNTs. We have studied the influence that pH and electrolytes have in determining the colloidal stability of oxidized multiwalled carbon nanotubes (O-MWCNTs), prepared by refluxing pristine MWCNTs in nitric acid. The acid-treated MWCNTs contained oxygen predominantly in the form of carboxyl groups. Colloidal suspensions of O-MWCNTs were prepared by low-power sonication and contained negatively charged, individual MWCNTs with an average length of approximately 650 nm. Time-resolved dynamic light scattering revealed that the aggregation rate of O-MWCNTs exhibited both reaction and mass-transport limited regimes in the presence of different electrolytes and as a function of pH. Particle stability profiles constructed from aggregation rate data allowed for the determination of critical coagulation concentrations (CCC), a metric of colloidal stability. The CCC values of O-MWCNTs varied with counterion concentration and valence in a manner consistentwith DLVO theory. Potentiometric measurements of surface charge correlated well with the observed pH-dependent variations in the O-MWCNT's colloidal stability. Electrophoretic mobility was also a diagnostic of particle stability, but only in neutral and acidic conditions.