gebhart/patrepeval huggingface dataset:
Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA (2004)

We use lentiviral-delivered RNA interference (RNAi) to inhibit the growth of a model of primary effusion lymphoma (PEL) in vitro and in vivo. RNAi is a phenomenon allowing the sequence-specific targeting and silencing of exogenous and endogenous gene expression and is being applied to inhibit viral replication both in vitro and in vivo. We show that silencing of genes believed to be essential for the Kaposi sarcoma-associated herpesvirus (KSHV) latent life cycle (the oncogenic cluster) has a varied effect in PEL cell lines cultured in vitro, however, concomitant silencing of the viral cyclin (vcyclin) and viral FLICE (Fas-associating protein with death domain-like interleukin-1beta-converting enzyme) inhibitory protein (vFLIP) caused efficient apoptosis in all PEL lines tested. We demonstrate that in a murine model of PEL, lentiviral-mediated RNA interference both inhibits development of ascites and can act as a treatment for established ascites. We also show that the administered lentiviral vectors are essentially limited to the peritoneal cavity, which has advantages for safety and dosage in a therapeutic setting. This shows the use of lentiviral-mediated RNA interference in vivo as a potential therapeutic against a virally driven human cancer.
1,25‐Dihydroxyvitamin D<sub>3</sub> is a potent inducer of nerve growth factor synthesis (1991)

1,25-Dihydroxyvitamin D3 (1,25-(OH)2D3), a metabolically active form of vitamin D, is shown to increase in a dose-dependent manner the cellular pool of NGF mRNA in murine L-929 fibroblasts cultured in a serum-free medium. This effect can be detected as early as 3 hours after 1,25-(OH)2D3 addition and persists for at least 28 hours. It is accompanied by an enhancement of the amount of NGF protein secreted in the culture medium. Since the proto-oncogene c-fos appears involved in the regulation of the NGF gene (Mocchetti et al.: Proceedings of the National Academy of Sciences of the United States of America 86: 3871-895, 1989; Hengerer et al: Proceedings of the National Academy of Sciences of the United States of America 87:3899-3903, 1990), the effect of 1,25-(OH)2D3 on c-fos expression was analysed and compared to that elicited by other inducers of the NGF gene, serum (Wion et al: FEBS Letters 189:37-41, 1985) and phorbol 12-myristate 13-acetate (PMA) (Wion et al: FEBS Letters 262:42-44, 1990). Addition of serum or PMA to L-929 cells was rapidly followed by a transient activation of the c-fos gene. In contrast, c-fos transcripts remained undetected in the presence of 1,25-(OH)2D3. The failure to find any evidence of c-fos expression suggests that 1,25-(OH)2D3 could enhance the pool of NGF mRNA by a mechanism independent of the c-fos pathway.(ABSTRACT TRUNCATED AT 250 WORDS)
Library Support in an Actor-Based Parallel Programming Platform (2011)

Actor model-based design is actively researched for parallel embedded SW design since the model exposes the potential parallelism explicitly in an architecture-neutral form. In most actor-oriented models, actors are self-contained and data channels are the only sharable object between actors, and they compose a system in a flat layer. In contrast, it is common to use shared library functions and construct vertically layered software for efficiency and modularity. To fill this gap between modeling and implementation, we propose a special actor, library task, with new types of ports: library master port and library slave port. It is a sharable and mappable object that defines a set of function interfaces inside. N:1 master-slave connection allows sharing a library task and the master-slave connection can specify vertically layered software and client-server applications naturally. To support the library task in our embedded software design environment, we develop an automatic mapping algorithm as well as an automatic code generator. The design environment with the library task is applied for two target platforms: IBM CELL Broad band Engine and an ARM-based multicore simulator. Preliminary experiments show that the special actor, or library task, extends the expression power of the previous actor model with efficiently generated codes.
Effects of corticosteroids on bronchodilator action in chronic obstructive lung disease. (1992)

In non-allergic subjects with chronic obstructive lung disease short term treatment with high doses of inhaled or oral corticosteroids does not modify the bronchodilator response to salbutamol or ipratropium or the protection provided by either drug against histamine. Salbutamol produces greater protection from histamine induced bronchoconstriction than ipratropium.
Anticoagulation Monitoring during Cardiac Surgery  (1999)

The literature does not consistently support the importance of anticoagulation monitoring techniques during CPB. This is best reflected by studies that have evaluated the impact of the ACT method on blood loss and transfusion outcomes. Inconsistent findings from studies that evaluated the impact of ACT monitoring may be related to either suboptimal study design (i.e., retrospective, unblinded, nonrandomized) or possibly the diagnostic inprecision of the ACT method used in these studies. There are a small number of well-controlled studies, some of which suggest that bleeding and transfusion outcomes can be improved by refining heparin monitoring techniques, either by sustaining better anticoagulation during CPB or by optimizing protamine doses (i.e., when empiric protocols result in excessive protamine doses). More well-controlled studies are needed to better define the importance of anticoagulation management during CPB.
Dinuclear Complexes Containing Ferrocenyl and Oxomolybdenum(V) Groups Linked by Conjugated Bridges:  A New Class of Electrochromic Near-Infrared Dye (1998)

Dinuclear complexes with ferrocenyl and oxomolybdenum(V) fragments linked by a conjugated spacer have been prepared and studied by spectroelectrochemistry; the first oxidation (ferrocene → ferrocenium) affords spectra characteristic of localized ferrocenium and oxo-Mo(V) fragments, but the second oxidation [Mo(V)→Mo(VI)] results in very intense new transitions in the near-IR region which we ascribe to a fully allowed π → π* transition of the bridging group, which now has quinonoidal character.
Electrochemical Sensors (2012)

This chapter contains sections titled: Basic Principles Carbon Nanotube Field-effect Transistors Chemical Resistors Temperature Sensors Smart Textiles Molecularly Imprinted Polymers Other Analytes
Non-linear multivariable predictive control of an alcoholic fermentation process using functional link networks (2005)

In this work a MIMO non-linear predictive controller was developed for an extractive alcoholic fermentation process. The internal model of the controller was represented by two MISO Functional Link Networks (FLNs), identified using simulated data generated from a deterministic mathematical model whose kinetic parameters were determined experimentally. The FLN structure presents as advantages fast training and guaranteed convergence, since the estimation of the weights is a linear optimization problem. Besides, the elimination of non-significant weights generates parsimonious models, which allows for fast execution in an MPC-based algorithm. The proposed algorithm showed good potential in identification and control of non-linear processes.
A T-cell response to a liver-stage <i>Plasmodium</i> antigen is not boosted by repeated sporozoite immunizations (2013)

Development of an antimalarial subunit vaccine inducing protective cytotoxic T lymphocyte (CTL)-mediated immunity could pave the way for malaria eradication. Experimental immunization with sporozoites induces this type of protective response, but the extremely large number of proteins expressed by Plasmodium parasites has so far prohibited the identification of sufficient discrete T-cell antigens to develop subunit vaccines that produce sterile immunity. Here, using mice singly immunized with Plasmodium yoelii sporozoites and high-throughput screening, we identified a unique CTL response against the parasite ribosomal L3 protein. Unlike CTL responses to the circumsporozoite protein (CSP), the population of L3-specific CTLs was not expanded by multiple sporozoite immunizations. CSP is abundant in the sporozoite itself, whereas L3 expression does not increase until the liver stage. The response induced by a single immunization with sporozoites reduces the parasite load in the liver so greatly during subsequent immunizations that L3-specific responses are only generated during the primary exposure. Functional L3-specific CTLs can, however, be expanded by heterologous prime-boost regimens. Thus, although repeat sporozoite immunization expands responses to preformed antigens like CSP that are present in the sporozoite itself, this immunization strategy may not expand CTLs targeting parasite proteins that are synthesized later. Heterologous strategies may be needed to increase CTL responses across the entire spectrum of Plasmodium liver-stage proteins.
High-performance of deep eutectic solvent based aqueous bi-phasic systems for the extraction of DNA (2016)

DES-based ABSs were initially applied to extraction and back extraction of DNA. The remarkable performance on selective separation of DNA and protein was evaluated. The binding characteristics and mechanism of extraction process were investigated.
Target Gene Specificity of E2F and Pocket Protein Family Members in Living Cells (2000)

E2F-mediated transcription is thought to involve binding of an E2F-pocket protein complex to promoters in the G(0) phase of the cell cycle and release of the pocket protein in late G(1), followed by release of E2F in S phase. We have tested this model by monitoring protein-DNA interactions in living cells using a formaldehyde cross-linking and immunoprecipitation assay. We find that E2F target genes are bound by distinct E2F-pocket protein complexes which change as cells progress through the cell cycle. We also find that certain E2F target gene promoters are bound by pocket proteins when such promoters are transcriptionally active. Our data indicate that the current model applies only to certain E2F target genes and suggest that Rb family members may regulate transcription in both G(0) and S phases. Finally, we find that a given promoter can be bound by one of several different E2F-pocket protein complexes at a given time in the cell cycle, suggesting that cell cycle-regulated transcription is a stochastic, not a predetermined, process.
What decline in pain intensity is meaningful to patients with acute pain? (2003)

Despite widespread use of the 0-10 numeric rating scale (NRS) of pain intensity, relatively little is known about the meaning of decreases in pain intensity assessed by means of this scale to patients. We aimed to establish the meaning to patients of declines in pain intensity and percent pain reduction. Upon arrival to the postanesthesia care unit, postsurgical patients rated their baseline pain intensity on both a 0-10 NRS and on a 4-point verbal scale. Patients whose NRS was higher than 4/10 received intravenous opioids until their pain intensity declined to 4/10 or lower. During opioid titration, patients were asked every 10 min to rate pain intensity on a NRS and to indicate the degree of pain improvement on a 5-point Likert scale from 'no improvement' to 'complete pain relief'. Seven hundred adult patients were enrolled. For patients with moderate pain, a decrease of 1.3 units (20% reduction) corresponded to 'minimal' improvement, a decrease of 2.4 (35% reduction) to 'much' improvement, a decrease of 3.5 units (45% reduction) corresponded to 'very much' improvement. For patients with severe pain, the decrease in NRS pain score and the percentage of pain relief had to be larger to obtain similar degrees of pain relief. The change in pain intensity that is meaningful to patients increases as the severity of their baseline pain increases. The present findings are applicable in the clinical setting and research arena to assess treatment efficacy.
Interactive virtual 3D gallery using motion detection of mobile device (2011)

Abstract- Normally all digital images are display in a flat two-dimensional (2D) image space. However, the three dimensional (3D) techniques exist that allow users to interact and walk through a virtual environment in mobile device using openglES. So in this paper, an interactive virtual three dimensional (3D) photo gallery on mobile device is presented. The system will allow users to take pictures with the mobile device and exhibit in the form of virtual 3D gallery and navigate or walk through in the gallery by pressing the button or moving the device. The device has the computational unit which is capable of determining motion data from the g-sensor or accelerometer sensor. The motion data describes the movement of the device including a rotation of the device. Therefore, the benefit of the sensor could be applied such as modifying the view displayed on the screen vertically or horizontally automatically. Also the system can detect tilting left – right device for viewing image and navigating in the virtual 3D Gallery as well. The result shows that the interactive virtual 3D photo gallery contains digital photos taken by a user and the user can navigates through the gallery by pressing buttons or moving the mobile device.
Identifying task-based sessions in search engine query logs (2011)

The research challenge addressed in this paper is to devise effective techniques for identifying task-based sessions, i.e. sets of possibly non contiguous queries issued by the user of a Web Search Engine for carrying out a given task. In order to evaluate and compare different approaches, we built, by means of a manual labeling process, a ground-truth where the queries of a given query log have been grouped in tasks. Our analysis of this ground-truth shows that users tend to perform more than one task at the same time, since about 75% of the submitted queries involve a multi-tasking activity. We formally define the Task-based Session Discovery Problem (TSDP) as the problem of best approximating the manually annotated tasks, and we propose several variants of well known clustering algorithms, as well as a novel efficient heuristic algorithm, specifically tuned for solving the TSDP. These algorithms also exploit the collaborative knowledge collected by Wiktionary and Wikipedia for detecting query pairs that are not similar from a lexical content point of view, but actually semantically related. The proposed algorithms have been evaluated on the above ground-truth, and are shown to perform better than state-of-the-art approaches, because they effectively take into account the multi-tasking behavior of users.
A study of the timing properties of Cd/sub 0.9/Zn/sub 0.1/Te (2002)

CdZnTe has become a material of great interest in the field of X- and /spl gamma/-ray imaging, and shows great promise as a highly efficient, room-temperature operation detector. However, data on the timing resolution obtainable with this material is scarce. It is known that CdZnTe, in common with all compound semi-conductors, gives pulses of varying rise-time depending on the interaction location, hence causing a broadening of the time spectrum. We therefore assembled and characterized an appropriate electronic measuring set-up and took data with 2 different sets of detectors, varying the experimental parameters such as bias voltage, threshold and temperature during the course of the experiment. The results obtained with planar detectors of 9 mm/sup 2//spl times/2 mm thick were superior to the results obtained with detectors of 25 mm/sup 2//spl times/10 mm thick, even when the difference in effective bias field strength is considered. Timing resolutions of 5.3 ns and 20.9 ns (FWHM) respectively were obtained. Reducing the temperature to 0/spl deg/C reduced the resolution to 4.5 ns, thereby indicating that the performance is limited by signal-to-noise ratio considerations.
Bioactivity Profiling with Parallel Mass Spectrometry Reveals an Assemblage of Green Tea Metabolites Affording Protection against Human Huntingtin and α-Synuclein Toxicity (2007)

Aberrant protein aggregation and misfolding are key pathological features of many neurodegenerative disorders, including Huntington's and Parkinson's diseases. Compounds that offer protection from toxicity associated with aggregation-prone neurodegenerative proteins may have applications for the treatment of a multitude of disorders. A high-throughput bioassay system with parallel electrospray ionization mass spectrometry screening has been designed for critical evaluation of milligram quantities of natural product extracts, including dietary substances, for compounds of pharmacological relevance to the treatment of human neurodegenerative diseases. Using Saccharomyces cerevisiae strains engineered to express mutant human huntingtin and alpha-synuclein, we are able to identify extracts and compounds that protect cells from toxicity associated with these proteins. Applying this screening paradigm, we determined that a bioactive green tea extract contains an assemblage of catechins that were individually characterized for their respective protective effects against huntingtin and alpha-synuclein toxicity.
Stabilization of Copper Catalysts for Liquid‐Phase Reactions by Atomic Layer Deposition (2013)

Abstract Atomic layer deposition (ALD) of an alumina overcoat can stabilize a base metal catalyst (e.g., copper) for liquid‐phase catalytic reactions (e.g., hydrogenation of biomass‐derived furfural in alcoholic solvents or water), thereby eliminating the deactivation of conventional catalysts by sintering and leaching. This method of catalyst stabilization alleviates the need to employ precious metals (e.g., platinum) in liquid‐phase catalytic processing. The alumina overcoat initially covers the catalyst surface completely. By using solid state NMR spectroscopy, X‐ray diffraction, and electron microscopy, it was shown that high temperature treatment opens porosity in the overcoat by forming crystallites of γ‐Al 2 O 3 . Infrared spectroscopic measurements and scanning tunneling microscopy studies of trimethylaluminum ALD on copper show that the remarkable stability imparted to the nanoparticles arises from selective armoring of under‐coordinated copper atoms on the nanoparticle surface.
Extracellular Proteases of<i>Trichoderma</i>Species (2005)

Cellulolytic, xylanolytic, chitinolytic and beta-1,3-glucanolytic enzyme systems of species belonging to the filamentous fungal genus Trichoderma have been investigated in details and are well characterised. The ability of Trichoderma strains to produce extracellular proteases has also been known for a long time, however, the proteolytic enzyme system is relatively unknown in this genus. Fortunately, in the recent years more and more attention is focused on the research in this field. The role of Trichoderma proteases in the biological control of plant pathogenic fungi and nematodes has been demonstrated, and it is also suspected that they may be important for the competitive saprophytic ability of green mould isolates and may represent potential virulence factors of Trichoderma strains as emerging fungal pathogens of clinical importance. The aim of this review is to summarize the information available about the extracellular proteases of Trichoderma. Numerous studies are available about the extracellular proteolytic enzyme profiles of Trichoderma strains and about the effect of abiotic environmental factors on protease activities. A number of protease enzymes have been purified to homogeneity and some protease encoding genes have been cloned and characterized. These results will be reviewed and the role of Trichoderma proteases in biological control as well as their advantages and disadvantages in biotechnology will be discussed.
Flatback Airfoil Wind Tunnel Experiment (2008)

An experimental investigation of blunt trailing edge or flatback airfoils was conducted in the University of California, Davis Aeronautical Wind Tunnel. The flatback airfoil was created by symmetrically adding thickness to both sides of the camber line of a baseline airfoil, while maintaining the maximum thickness-to-chord ratio of 35%. Three airfoils, with geometries based on the baseline airfoil, of various trailing edge thicknesses (0.5%, 8.75%, and 17.5% chord) are discussed in this report. In the present study, each airfoil was tested under free and fixed boundary layer transition flow conditions at Reynolds numbers of 333,000 and 666,000. The fixed transition conditions, used to simulate surface soiling effects, were achieved by placing artificial tripping devices at 2% chord on the suction surface and 5% chord on the pressure surface of each airfoil. The results of this investigation show the blunt trailing edge airfoils reduced the well-documented sensitivity to leading edge transition for thick airfoils. The nominally sharp trailing edge airfoil, with trailing edge thickness of 0.5% chord, performed well under free transition conditions, but the lift characteristics deteriorated significantly when the flow was tripped at the leading edge. As the trailing edge thickness was increased, the effect of leading edge transition diminished, that is, the airfoil lift performance became increasingly similar for free and fixed transition. The flatback airfoils yield increased drag coefficients over the sharp trailing edge airfoil due to an increase in base drag. To address the base drag increment, six different trailing edge devices were investigated for the airfoil with 17.5% chord trailing edge thickness at a Reynolds number of 333,000 under tripped flow conditions. Several of the trailing edge devices caused significant reductions in base drag.
Cyclins A and B associate with chromatin and the polar regions of spindles, respectively, and do not undergo complete degradation at anaphase in syncytial Drosophila embryos. (1992)

Maternally contributed cyclin A and B proteins are initially distributed uniformly throughout the syncytial Drosophila embryo. As dividing nuclei migrate to the cortex of the embryo, the A and B cyclins become concentrated in surface layers extending to depths of approximately 30-40 microns and 5-10 microns, respectively. The initiation of nuclear envelope breakdown, spindle formation, and the initial congression of the centromeric regions of the chromosomes onto the metaphase plate all take place within the surface layer occupied by cyclin B on the apical side of the blastoderm nuclei. Cyclin B is seen mainly, but not exclusively, in the vicinity of microtubules throughout the mitotic cycle. It is most conspicuous around the centrosomes. Cyclin A is present at its highest concentrations throughout the cytoplasm during the interphase periods of the blastoderm cycles, although weak punctate staining can also be detected in the nucleus. It associates with the condensing chromosomes during prophase, segregates into daughter nuclei in association with chromosomes during anaphase, to redistribute into the cytoplasm after telophase. In contrast to the cycles following cellularization, neither cyclin is completely degraded upon the metaphase-anaphase transition.
Rollable QVGA Active-Matrix Displays Based on Organic Electronics (2005)

<p>A 100μm thin QVGA active-matrix electrophoretic display is produced using organic transistors. The display can be bent to a radius less than 1 cm without any performance loss. Higher contrast and low crosstalk was achieved by addition of a field shield to our backplane</p>