gebhart/patrepeval huggingface dataset:
High Performance Control Design for Dynamic Voltage Scaling Devices (2011)

Dynamic voltage scaling (DVS) is an important method in managing dynamically the system supply voltage for efficient power reduction. This approach is applied in very large scale integration (VLSI). A dc-dc converter is an electronic device which allows to vary the voltage and, thus, to implement DVS technique. In this paper, a high-performance controller is presented for a novel discrete DVS converter. This controller is developed with the aim to deal with the unknown resistive component of the load as well as to minimize the dissipated energy and current peaks, what is very important in the field of microelectronics. Current peaks and power consumption are minimized by computing an optimal evolution for the voltage reference. Likewise, an adaptive controller is proposed to deal with the unknown load resistive parameter. Consequently, the obtained advanced controller can acquires a high consideration on electronic devices.
High-Performance MIM Capacitors for a Secondary Power Supply Application (2018)

Microstructure is important to the development of energy devices with high performance. In this work, a three-dimensional Si-based metal-insulator-metal (MIM) capacitor has been reported, which is fabricated by microelectromechanical systems (MEMS) technology. Area enlargement is achieved by forming deep trenches in a silicon substrate using the deep reactive ion etching method. The results indicate that an area of 2.45 × 10³ mm² can be realized in the deep trench structure with a high aspect ratio of 30:1. Subsequently, a dielectric Al₂O₃ layer and electrode W/TiN layers are deposited by atomic layer deposition. The obtained capacitor has superior performance, such as a high breakdown voltage (34.1 V), a moderate energy density (≥1.23 mJ/cm²) per unit planar area, a high breakdown electric field (6.1 ± 0.1 MV/cm), a low leakage current (10<sup>-7</sup> A/cm² at 22.5 V), and a low quadratic voltage coefficient of capacitance (VCC) (≤63.1 ppm/V²). In addition, the device's performance has been theoretically examined. The results show that the high energy supply and small leakage current can be attributed to the Poole⁻Frenkel emission in the high-field region and the trap-assisted tunneling in the low-field region. The reported capacitor has potential application as a secondary power supply.
Tetraspanins in Extracellular Vesicle Formation and Function (2014)

Extracellular vesicles (EVs) represent a novel mechanism of intercellular communication as vehicles for intercellular transfer of functional membrane and cytosolic proteins, lipids, and RNAs. Microvesicles, ectosomes, shedding vesicles, microparticles, and exosomes are the most common terms to refer to the different kinds of EVs based on their origin, composition, size, and density. Exosomes have an endosomal origin and are released by many different cell types, participating in different physiological and/or pathological processes. Depending on their origin, they can alter the fate of recipient cells according to the information transferred. In the last two decades, EVs have become the focus of many studies because of their putative use as non-invasive biomarkers and their potential in bioengineering and clinical applications. In order to exploit this ability of EVs many aspects of their biology should be deciphered. Here, we review the mechanisms involved in EV biogenesis, assembly, recruitment of selected proteins, and genetic material as well as the uptake mechanisms by target cells in an effort to understand EV functions and their utility in clinical applications. In these contexts, the role of proteins from the tetraspanin superfamily, which are among the most abundant membrane proteins of EVs, will be highlighted.
Identification and Characterization of the Bel 3 Protein of Human Foamy Virus (1994)

The human foamy virus (HFV) is a complex retrovirus that contains several regulatory and auxiliary bel genes besides the gag, pol, and env genes. In contrast to the gene products of bel 1 and bel 2/bet that were identified previously, the Bel 3 protein has not been described to date. Here we report the identification of Bel 3 in HFV-infected cells by immunoprecipitation, indirect immunofluorescence, and expression cloning under the control of a strong heterologous promoter. Bel 3 was immunoprecipitated with an antiserum directed against a bacterially expressed and purified form of recombinant Bel 3 antigen. Bel 3 was found to be expressed in low amounts in the cytoplasm of HFV-infected cells and to migrate with an apparent molecular mass of 19.4 kDa on electrophoresis in SDS-polyacrylamide gels, consistent with the calculated value of 18.2 kDa. Radioimmunoprecipitation of HFV-infected cell lysates with the hyperimmune serum against Bel 3 revealed at least two additional immunoreactive bands of 15.5 and 10.6 kDa. The results indicate that Bel 3 was labile, because it was partially degraded even at early time points after infection. On transfection and expression in transfected COS cells, recombinant Bel 3 was immunoprecipitated and migrated in three polypeptide bands of 18.7, 14.8, and 9.3 kDa under denaturing conditions. In the absence of reducing agents, the bacterially expressed and purified recombinant Bel 3 protein of 16.1 kDa can form homodimers of 30 kDa.
Whole-genome sequencing and microarray analysis of ex vivo <i>Plasmodium vivax</i> reveal selective pressure on putative drug resistance genes (2010)

Plasmodium vivax causes 25-40% of malaria cases worldwide, yet research on this human malaria parasite has been neglected. Nevertheless, the recent publication of the P. vivax reference genome now allows genomics and systems biology approaches to be applied to this pathogen. We show here that whole-genome analysis of the parasite can be achieved directly from ex vivo-isolated parasites, without the need for in vitro propagation. A single isolate of P. vivax obtained from a febrile patient with clinical malaria from Peru was subjected to whole-genome sequencing (30× coverage). This analysis revealed over 18,261 single-nucleotide polymorphisms (SNPs), 6,257 of which were further validated using a tiling microarray. Within core chromosomal genes we find that one SNP per every 985 bases of coding sequence distinguishes this recent Peruvian isolate, designated IQ07, from the reference Salvador I strain obtained in 1972. This full-genome sequence of an uncultured P. vivax isolate shows that the same regions with low numbers of aligned sequencing reads are also highly variable by genomic microarray analysis. Finally, we show that the genes containing the largest ratio of nonsynonymous-to-synonymous SNPs include two AP2 transcription factors and the P. vivax multidrug resistance-associated protein (PvMRP1), an ABC transporter shown to be associated with quinoline and antifolate tolerance in Plasmodium falciparum. This analysis provides a data set for comparative analysis with important potential for identifying markers for global parasite diversity and drug resistance mapping studies.
Association of <scp>RNASEL</scp> and 8q24 variants with the presence and aggressiveness of hereditary and sporadic prostate cancer in a hispanic population (2013)

To study the association between the polymorphisms Arg462Gln and Asp541Glu from the RNASEL gene (1q25), and the polymorphisms rs620861, rs1447295, rs6983267, rs7837328 from the chromosome 8q24 with the risk of presenting prostate cancer (PCa) and its clinical characteristics in a Hispanic (Chilean) population. The study was performed on 21 control patients and 83 patients diagnosed with PCa. Polymorphisms were analysed from blood samples through real-time PCR by using TaqMan probes, and the genetic analysis was performed with the SNPStats program. Also, a comparison was performed between clinical characteristics of PCa and the presence of the different polymorphism genotypes by using the Minitab software. There was a significant association between the genotype G/G from the polymorphism rs6983267 with an overall increased risk of PCa, in patients both with or without family history of PCa (OR = 4.47, 95% CI = 1.05-18.94, P = 0.034 and OR = 3.57, 95% CI = 0.96-13.35, P = 0.037, respectively). Regarding clinical parameters, patients carrying the genotype C/C from the polymorphism Asp541Glu had significantly higher prostate-specific antigen (PSA) levels than patients carrying the other genotypes (P = 0.034). Moreover, patients with the genotype G/G of rs6983267 had higher PSA levels (P = 0.024). The polymorphism rs6983267 from region 3 of the chromosome 8q24 appears to be a prominent risk factor for PCa and a biomarker for cancer aggressiveness in the group of patients who presented higher levels of PSA at the time of diagnosis.
Heck Reaction in Ionic Liquids and the in Situ Identification of <i>N-</i>Heterocyclic Carbene Complexes of Palladium (2000)

The Heck reaction was found to proceed markedly more efficiently in the ionic liquid 1-butyl-3-methylimidazolium bromide ([bmim][Br]) than in the analogous tetrafluoroborate salt ([bmim][BF4]). In relation to this observation, 1-butyl-3-methylimidazol-2-ylidene (bmiy) complexes of palladium [PdBr(μ-Br)(bmiy)]2 (1) and [PdBr2(bmiy)2] (2) have been isolated in [bmim][Br] but not in [bmim][BF4] under conditions similar to those employed for the Heck reaction. In both the ionic liquid [bmim][Br] and molecular solvents, complex 2 was formed via the intermediate 1. 2 has been shown to be active in the Heck reaction in [bmim][Br] but much less so in [bmim][BF4] due to transformation by the latter into less active species.
Mechanical Properties and Flame Retardancy of SEBS-based Composites Filled with Magnesium Hydroxide (2009)

The flame-retardancy and mechanical properties of styrene-ethylene/butylene-styrene block copolymer (SEBS) based composites, containing SEBS blended with a paraffinic hydrocarbon extender oil, copolymer polypropylene (PP), and magnesium hydroxide (MH) or silane coupling agent surface-treated magnesium hydroxide (m-MH) were investigated. A maleic anhydride-grafted SEBS (MA-SEBS) was used as a polymer modifier for some composites. As the amount of MH or m-MH increased, the flame-retardancy of composites improved, while the tensile strength and elongation at break decreased, and the melt property of the composites became worse as well. When the oil-filled SEBS (O-SEBS) was partially replaced by MA-SEBS, the tensile strength of the composites increased, and elongation at break decreased. Furthermore replacing O-SEBS with MA-SEBS and treating MH with silane coupling agent (simultaneously) improved the tensile strength of the composites by a large margin. The phase structure of the composites was characterised using scanning electron microscopy (SEM) and this revealed that the particle-matrix interfacial bonding was improved through the addition of MA-SEBS. The stability of the composites was investigated by thermogravimetric analysis (TGA).
Chitosan-<i>graft</i>-6-mercaptonicotinic Acid: Synthesis, Characterization, and Biocompatibility (2009)

Thiolated chitosans are relatively new thiolated biopolymers exhibiting mucoadhesive, enzyme inhibitory, and permeation enhancing properties. A drawback, however, is their pH dependent reactivity. The aim of this study was therefore to develop a novel thiolated chitosan showing a non pH dependent reactivity of its thiol groups. For this purpose, 6-mercaptonicotinic acid (6-MNA) was covalently attached to chitosan by a carbodiimide mediated reaction. The obtained conjugate was characterized in vitro by quantification of immobilized thiol groups, cytotoxicity, in situ gelling properties, and disulfide bond formation at different pH. The synthesized conjugate displayed up to 973.80 micromol thiol groups per gram of polymer in reduced form. The polymer was nontoxic and showed in situ gelling properties. Furthermore, disulfide bond formation and therefore gelling properties occurred at various pH ranges. The reactivity of thiol groups was in the same range at pH 3 and pH 6.8. According to these results, chitosan-6 mercaptonicotinic acid seems to have some promising features to be used particularly for mucoadhesive formulations.
Solubilizing Buried Domains of Proteins: A Self-Assembling Interface Domain from Glutathione Reductase (1994)

In the dimeric glutathione reductase (GR) from Escherichia coli, the interface domain is largely surrounded by the other three domains in each subunit of the protein. Subgenes encoding three forms of the interface domain have been expressed in E. coli and the products purified from inclusion bodies: INT is the excised interface domain, as it is found in native GR; INTN and INTFN are variants carrying exchanges of surface residues in what would have been hydrophobic contact regions with other neighboring domains. The isolated INT domain was found to be a soluble and folded protein, but it was isolated as a mixture of the dimer and at least two species of higher molecular weight. The latter were believed to arise by further association of the dimer via the newly exposed and unsatisfied hydrophobic contact regions. In the variant INTN, three hydrophobic residues normally involved in the contact with the NADPH-binding domain in GR were replaced. This partly suppressed the further aggregation of the dimers. However, continued aggregation at high protein concentrations suggested that at least one further site of unwanted aggregation was still present. After four additional amino acid replacements in the region normally in contact with the FAD-binding domain, the resulting variant INTFN exhibited no unspecific aggregation, even at concentrations as high as 3.2 mg/mL.(ABSTRACT TRUNCATED AT 250 WORDS)
Cost-Effective Crowdsourced Entity Resolution (2016)

Crowdsourced entity resolution has recently attracted significant attentions because it can harness the wisdom of crowd to improve the quality of entity resolution. However existing techniques either cannot achieve high quality or incur huge monetary costs. To address these problems, we propose a cost-effective crowdsourced entity resolution framework, which significantly reduces the monetary cost while keeping high quality. We first define a partial order on the pairs of records. Then we select a pair as a question and ask the crowd to check whether the records in the pair refer to the same entity. After getting the answer of this pair, we infer the answers of other pairs based on the partial order. Next we iteratively select pairs without answers to ask until we get the answers of all pairs. We devise effective algorithms to judiciously select the pairs to ask in order to minimize the number of asked pairs. To further reduce the cost, we propose a grouping technique to group the pairs and we only ask one pair instead of all pairs in each group. We develop error-tolerant techniques to tolerate the errors introduced by the partial order and the crowd. Experimental results show that our method reduces the cost to 1.25% of existing approaches (or existing approaches take 80* monetary cost of our method) while not sacrificing the quality.
El emplazamiento económico de Austria en el panorama internacional (2002)

En este artículo se pone de relieve la presencia de empresas multinacionales en Austria debido a las buenas infraestructuras, al capital humano altamente cualificado, clima social y laboral estable y tranquilo, procesos de liberalización e incremento de la producción, siendo una de las preocupaciones principales la investigación para la innovación
Usable AI Requires Commonsense Knowledge (2008)

Artificial Intelligence techniques are increasingly being applied to the user interface, as evidenced by growing numbers of CHI papers which have some AI aspect, and standalone conferences on the subject, such as the Intelligent User Interfaces (IUI) Conference (and this workshop!). I argue that an important, but underappreciated component for assuring the adherence of AI interfaces to CHI principles for usable interfaces, is capturing Commonsense knowledge. Commonsense knowledge can be viewed as a collection of simple facts about people and everyday life, such as &quot;Things fall down, not up&quot;, and &quot;People eat breakfast in the morning&quot;. One reason that conventional interfaces are stupid and frustrating to use, is that they lack such knowledge. At the MIT Media Lab, we have a large body of experience in creating applications across a wide variety of domains that make use of such knowledge [6]. We distill from our experience some principles for application of Commonsense knowledge to make interfaces more usable.
The Molecular Organization of Lipids in the Membrane of <i>Escherichia coli:</i> Phase Transitions (1971)

X-ray diffraction techniques have been used to investigate the physical state of the lipids in the membrane of an unsaturated fatty acid auxotroph of Escherichia coli. Thermotropic phase transitions have been detected in membranes prepared from cells grown on various fatty acid supplements. Below the transition temperature, the X-ray diffraction pattern features a sharp ring at 420 pm (4.2 A) due to the close hexagonal packing of the apolar groups of the lipids; above the transition temperature the lipids are in a less organized liquid-crystalline state that gives rise to a diffuse band centered at a Bragg spacing of 460 pm (4.6 A). This transition occurs at or below the temperature at which the cells were grown. Disparities between this transition temperature and the temperature of discontinuities in the Arrhenius plots for proline transport and succinic dehydrogenase activity lead us to conclude that the distribution of lipids within the membrane is heterogeneous.
Graft copolymerization onto starch. I. Complexes of Mn3 as initiators (1977)

Advantages in using the pyrophosphate complex of trivalent managanese over the sulfate complex as initiator for graft copolymerization onto starch are discussed. The first successful attempts to graft copolymerize acrylonitrile, methyl methacrylate, and acrylamide to starch and starch derivatives are described using managanic pyrophosphate as initiator. Selective solvent extraction of the reaction products and very low conversions of monomer to homopolymer in absence of starch substrates provide evidence for high grafting efficiencies obtained with acrylonitrile and methyl methacrylate. With acrylamide as monomer, however, low grafting efficiencies and considerable amounts of homopolymer are obtained under the experimental conditions investigated. Reaction mechanisms responsible for initiation of graft copolymerization are discussed. These are (a) glycol cleavage in the anhydroglucose units by Mn3+ ions leading to formation of a radical, and (b) enolization and further oxidation of oxidized starch by Mn3+ ions also leading to radical species. Mechanisms are also proposed for homopolymerization through Mn3+ oxidation of enols which probably are formed by “vinylogous” addition of water molecules to acrylamide and methyl methacrylate.
Rapid Screening of Environmental Chemicals for Estrogen Receptor Binding Capacity (1998)

Over the last few years, an increased awareness of endocrine disrupting chemicals (EDCs) and their potential to affect wildlife and humans has produced a demand for practical screening methods to identify endocrine activity in a wide range of environmental and industrial chemicals. While it is clear that in vivo methods will be required to identify adverse effects produced by these chemicals, in vitro assays can define particular mechanisms of action and have the potential to be employed as rapid and low-cost screens for use in large scale EDC screening programs. Traditional estrogen receptor (ER) binding assays are useful for characterizing a chemical's potential to be an estrogen-acting EDC, but they involve displacement of a radioactive ligand from crude receptor preparations at low temperatures. The usefulness of these assays for realistically determining the ER binding interactions of weakly estrogenic environmental and industrial compounds that have low aqueous solubility is unclear. In this report, we present a novel fluorescence polarization (FP) method that measures the capacity of a competitor chemical to displace a high affinity fluorescent ligand from purified, recombinant human ER-[alpha] at room temperature. The ER-[alpha] binding interactions generated for 15 natural and synthetic compounds were found to be similar to those determined with traditional receptor binding assays. We also discuss the potential to employ this FP technology to binding studies involving ER-ss and other receptors. Thus, the assay introduced in this study is a nonradioactive receptor binding method that shows promise as a high throughput screening method for large-scale testing of environmental and industrial chemicals for ER binding interactions.
Soil Tillage Management Affects Maize Grain Yield by Regulating Spatial Distribution Coordination of Roots, Soil Moisture and Nitrogen Status (2015)

The spatial distribution of the root system through the soil profile has an impact on moisture and nutrient uptake by plants, affecting growth and productivity. The spatial distribution of the roots, soil moisture, and fertility are affected by tillage practices. The combination of high soil density and the presence of a soil plow pan typically impede the growth of maize (Zea mays L.).We investigated the spatial distribution coordination of the root system, soil moisture, and N status in response to different soil tillage treatments (NT: no-tillage, RT: rotary-tillage, SS: subsoiling) and the subsequent impact on maize yield, and identify yield-increasing mechanisms and optimal soil tillage management practices. Field experiments were conducted on the Huang-Huai-Hai plain in China during 2011 and 2012. The SS and RT treatments significantly reduced soil bulk density in the top 0-20 cm layer of the soil profile, while SS significantly decreased soil bulk density in the 20-30 cm layer. Soil moisture in the 20-50 cm profile layer was significantly higher for the SS treatment compared to the RT and NT treatment. In the 0-20 cm topsoil layer, the NT treatment had higher soil moisture than the SS and RT treatments. Root length density of the SS treatment was significantly greater than density of the RT and NT treatments, as soil depth increased. Soil moisture was reduced in the soil profile where root concentration was high. SS had greater soil moisture depletion and a more concentration root system than RT and NT in deep soil. Our results suggest that the SS treatment improved the spatial distribution of root density, soil moisture and N states, thereby promoting the absorption of soil moisture and reducing N leaching via the root system in the 20-50 cm layer of the profile. Within the context of the SS treatment, a root architecture densely distributed deep into the soil profile, played a pivotal role in plants' ability to access nutrients and water. An optimal combination of deeper deployment of roots and resource (water and N) availability was realized where the soil was prone to leaching. The correlation between the depletion of resources and distribution of patchy roots endorsed the SS tillage practice. It resulted in significantly greater post-silking biomass and grain yield compared to the RT and NT treatments, for summer maize on the Huang-Huai-Hai plain.