gebhart/patrepeval huggingface dataset:
The sperm acrosomal matrix contains a novel member of the pentaxin family of calcium-dependent binding proteins. (1994)

The sperm acrosome is a regulated secretory granule that undergoes exocytosis during fertilization. To elucidate the structural organization of the contents within the acrosome, guinea pig sperm acrosomal apical segments were isolated and mapped by two-dimensional polyacrylamide gel electrophoresis (PAGE). Although complex, the two-dimensional PAGE map was dominated by two M(r) 50,000 polypeptides (p50 and proacrosin), a M(r) 67,000 polypeptide (p67), and a M(r) 32,000 polypeptide (sp32). Proacrosin (pI > 8.0), p67, and sp32 were extracted from apical segments by 1 M NaCl. Protein p50, a relatively acidic polypeptide, was not extracted in 1 M NaCl and/or 1% Triton X-100 at 4 degrees C, but was solubilized with 6 M urea. Protein p50 was purified from the urea extract by elution from DEAE-Sephacel with 100 mM guanidine HCl and appeared homogeneous by SDS-PAGE. Antibodies to p50 were monospecific as judged by Western blot analysis. Indirect immunofluorescence indicated that p50 was restricted to the acrosomal apical segment. Incubation of apical segments at pH 7.5 in the presence of 1 mM EDTA at 37 degrees C resulted in the release of p50 into the 200,000 x g supernatant fluid, a process that was reversed by a subsequent incubation with 1.5 mM CaCl2, but not with MgCl2. The Ca(2+)-dependent reassociation of p50 with the acrosomal apical segments was reversed by the addition of 2.0 mM EGTA, indicating that p50 binding is dependent on free Ca2+ concentrations. When acrosomal matrices were purified following Triton X-100 extraction, p50 was the major component, with p67, proacrosin, and sp32 as less prominent constituents. Molecular cloning demonstrated that p50 is a unique, testis-specific member of the pentaxin family of calcium-dependent binding proteins.
On-line scheduling in the presence of overload (2002)

The preemptive scheduling of sporadic tasks on a uniprocessor is considered. A task may arrive at any time, and is characterized by a value that reflects its importance, an execution time that is the amount of processor time needed to completely execute the task, and a deadline by which the task is to complete execution. The goal is to maximize the sum of the values of the completed tasks. An online scheduling algorithm that achieves optimal performance when the system is underloaded and provides a nontrivial performance guarantee when the system is overloaded is designed. The algorithm is implemented using simple data structures to run at a cost of O(log n) time per task, where n bounds the number of tasks in the system at any instant. Upper bounds on the best performance guarantee obtainable by an online algorithm in a variety of settings are derived.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Mutational analysis of ComS: evidence for the interaction of ComS and MecA in the regulation of competence development in <i>Bacillus subtilis</i> (1999)

The development of Bacillus subtilis genetic competence is a highly regulated adaptive response to stationary-phase stress. A key step in competence development is the activation of the transcriptional regulator ComK, which is required for the expression of genes encoding the products that function in DNA uptake. In log-phase cultures, ComK is trapped in a complex composed of MecA and ClpC, in which it is rendered inactive. The comS gene, contained within the srf operon, is induced in response to high culture cell density and nutritional stress. Its product functions to release active ComK from the complex, allowing ComK to stimulate the transcription initiation of its own gene as well as that of the late competence operons. Western analysis showed that ComS accumulates to maximal levels between T3 and T4, mirroring the pattern of competence cell development and late competence gene expression. Experiments to examine the target of ComS activity in vitro showed that ComS binds to MecA. This is further supported by coimmunoprecipitation using anti-MecA antiserum. To clarify the role of ComS in competence regulation, a system for evaluating the effect of comS and mutant derivatives on the expression of comG, one of the late competence operons, was constructed. comS mutations, created by alanine-scanning mutagenesis, that significantly reduced comG-lacZ expression were clustered within two regions, one at the N-terminus and the other at the C-terminus of ComS. ComSI13 --> A and ComSW43 --> A were selected for further analysis as representative mutants for both regions required for ComS activity. We observed that ComSI13 --> A showed significantly reduced affinity for MecA, whereas ComSW43 --> A showed near normal binding affinity for MecA. The results show that binding to MecA is critical for ComS function, but do not rule out the possibility that ComS possesses other activities.
Organic Matter Extraction from Soils with Water at High Pressure and Temperatures (1991)

Abstract The objective of this research was to develop a procedure for the more specific extraction of major organic matter (OM) components from soils. The method was tested on Ap horizons of a Haplaquoll, a Cryoboroll, and a Haploboroll, and on the Bh horizon of a Haplaquod. The four soils were extracted for 2 h with distilled water at a constant pressure of 17.2 MPa and at temperatures of 150, 200, and 250 °C. The OM extracts obtained were characterized by chemical methods, infrared (IR) and nuclear magnetic resonance (NMR) spectroscopy, and pyrolysis‐field ionization mass spectrometry (Py‐FIMS). With one exception, relatively more of the soil N (18–53%) than soil C (17–40%) was extracted from the four soils at the three temperatures. Recoveries of N at 200 and 250 °C were superior to those reported for extractions by 0.1 M Na 4 P 2 O 7 solution from the same soils, while C recoveries were higher from the Bainsville and Regina soils. In general, extracts obtained at 200 °C were richest in carbohydrates and especially in N components. The 13 C‐NMR spectra showed that most of the C in the extracts was aliphatic, occurring in the form of noncarbohydrate carbons bonded to OH and O. The Py‐FI mass spectra indicated that the extracts contained polysaccharides, n ‐fatty acids, n ‐alkanes, n ‐alcohols, sterols, N compounds (pyrroles and pyridines), and mono‐ and dilignins, all typical OM components. Extracts obtained at 150 °C were enriched in n ‐alcohols and diols, whereas extracts at 250 °C were rich in fatty acids. For the extraction of carbohydrates and N components, we recommend an extraction temperature of 200 °C; for soils rich in clay minerals, extractions at 250 °C may be more efficient. For studies on paraffinic OM components, extraction at 150 °C may be appropriate.
Super-resolution for real-time volumetric MR-temperature monitoring (2011)

Real time magnetic resonance (MR) thermometry provides continuous temperature mapping inside the human body and allows precise monitoring and control of thermal therapies. In practice it is still difficult to acquire images with 3D isotropic resolution. A high in-plane resolution is therefore preferred at the cost of the slice thickness, which may limit the accuracy of the thermometry.
The Actions and Metabolic Fate of Disulfiram (1981)

Due to the massive data sets available for drug candidates, modern drug discovery has advanced to the big data era. Central to this shift is the development of artificial intelligence approaches to implementing innovative modeling based on the dynamic, ...Read More
Cell biology of spinal cord injury and repair (2017)

Spinal cord injury (SCI) lesions present diverse challenges for repair strategies. Anatomically complete injuries require restoration of neural connectivity across lesions. Anatomically incomplete injuries may benefit from augmentation of spontaneous circuit reorganization. Here, we review SCI cell biology, which varies considerably across three different lesion-related tissue compartments: (a) non-neural lesion core, (b) astrocyte scar border, and (c) surrounding spared but reactive neural tissue. After SCI, axon growth and circuit reorganization are determined by neuron-cell-autonomous mechanisms and by interactions among neurons, glia, and immune and other cells. These interactions are shaped by both the presence and the absence of growth-modulating molecules, which vary markedly in different lesion compartments. The emerging understanding of how SCI cell biology differs across lesion compartments is fundamental to developing rationally targeted repair strategies.
M/sup 3/VDS-the cheapest, quickest, and least obtrusive means of providing multichannel domestic TV? (1988)

The authors advocate a new means of delivering multichannel TV to the home, using the as-yet untapped millimetre-wave region of the radio spectrum. A Millimetre-wave Multichannel Multipoint Video Distribution Service (M/sup 3/VDS) would be compatible with satellite broadcasting and complementary to cable TV by assisting expansion of fringe-area coverage and economic in-filling. In addition it offers an early and rapid expansion of the customer base for programming, since service can be quickly established over the complete coverage area without disruptive major engineering work. Recent gallium arsenide (GaAS) monolithic integrated circuit developments are the key to the system, now enabling cost effective MM-wave receivers to be built. >
NMR principle analysis based object detection for intelligent measurement of crude oil moisture content (2019)

Tracking crude oil moisture content in real time is critical in the oil refinery production process. Traditional methods are time-consuming, low precision and lack of mechanism analysis. Aiming to solve these problems, we present an object detection method of crude oil moisture content based on NMR principle and deep learning, which named convex region search. It realizes the location of water information in the crude oil 1H NMR spectrum and classification of all the sample points. Then corresponding regression models are established based on the captured features of disparate categories. The experimental results show that the proposed method can not only locate the position of water peak in NMR spectrum quickly and precisely, but also predict the moisture content value accurately.
Selective Oxidation of Alkanes and Ethers with Iodine Tris(trifluoroacetate) (1976)

Angewandte Chemie International Edition in EnglishVolume 15, Issue 7 p. 436-436 Communication Selective Oxidation of Alkanes and Ethers with Iodine Tris(trifluoroacetate)† Priv.-Doz. Dr. Joachim Buddrus, Priv.-Doz. Dr. Joachim Buddrus Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Search for more papers by this authorDipl.-Chem. Horst Plettenberg, Corresponding Author Dipl.-Chem. Horst Plettenberg Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Search for more papers by this author Priv.-Doz. Dr. Joachim Buddrus, Priv.-Doz. Dr. Joachim Buddrus Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Search for more papers by this authorDipl.-Chem. Horst Plettenberg, Corresponding Author Dipl.-Chem. Horst Plettenberg Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Institut für Spektrochemie und Angewandte Spektroskopie, Postfach 778, 4600 Dortmund 1 (Germany)Search for more papers by this author First published: July 1976 https://doi.org/10.1002/anie.197604361Citations: 28 † This work was supported by the Deutsche Forschungsgemeinschaft. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume15, Issue7July 1976Pages 436-436 RelatedInformation
Dynamical description of sinoatrial node pacemaking: improved mathematical model for primary pacemaker cell (2002)

We developed an improved mathematical model for a single primary pacemaker cell of the rabbit sinoatrial node. Original features of our model include 1) incorporation of the sustained inward current (I(st)) recently identified in primary pacemaker cells, 2) reformulation of voltage- and Ca(2+)-dependent inactivation of the L-type Ca(2+) channel current (I(Ca,L)), 3) new expressions for activation kinetics of the rapidly activating delayed rectifier K(+) channel current (I(Kr)), and 4) incorporation of the subsarcolemmal space as a diffusion barrier for Ca(2+). We compared the simulated dynamics of our model with those of previous models, as well as with experimental data, and examined whether the models could accurately simulate the effects of modulating sarcolemmal ionic currents or intracellular Ca(2+) dynamics on pacemaker activity. Our model represents significant improvements over the previous models, because it can 1) simulate whole cell voltage-clamp data for I(Ca,L), I(Kr), and I(st); 2) reproduce the waveshapes of spontaneous action potentials and ionic currents during action potential clamp recordings; and 3) mimic the effects of channel blockers or Ca(2+) buffers on pacemaker activity more accurately than the previous models.
Freezing Rates of Water Droplets (1975)

The freezing rates of water droplets have been calculated. The interfacial position is specified as a power series in time, and expressions for determining the first five coefficients are given. The five–term series solution is compared with an exact numerical one for a particular case, and the agreement is within 1% during most of the freezing process. For small droplets, the solution reduces to a previously published one, and the results of experiments in which the freezing times of droplets were measured confirm this.
Combining ability and heterosis for disease index of red rot in sugarcane (Saccharum of ficinarum L.) (2005)

Combining ability and heterosis for disease index of red rot were studied in the progenies of nine sugarcane (Saccharum of ficinarum L.) clones to identify suitable parents and crosses for further exploitation in breeding programmes. Based on the combining ability results, Co 8347, ISH-021 and Co 86011 were identified as good general combiners for imparting red rot resistance. Use of these clones, with high (negative) gca effects, would be advantageous in poly cross method of breeding. The association between sca effects and heterosis over mid-parent indicated that the sea effects were not so prominent to cause unidirectional heterosis. Hence, per se performance, sea effects and heterosis were considered to identify Co 7201 x Co 86011, Co 8347 x Co 1148, Co 89009 x Co 86011 and ISH-021 x Co 1148 as the best specific combinations for red rot disease index. Both additive and dominance variances were equally important and heritability (in narrow sense) was 0.51 for red rot resistance in sugarcane. High value (0.97) of heritability (in broad sense) indicated the negligible influence of environment on red rot disease development in sugarcane.
Synthesis and properties of di‐ and trinitrobenzyl substituted pyridine derivates (2010)

Abstract A new method to obtain di‐ and trinitrobenzyl substituted pyridines is presented in this paper. By systematic variation of reaction parameters, the reaction conditions were optimized. The novel synthesis circumvents the commonly used nitration of benzyl pyridines, and thus avoids the nitration of the heterocycle which is a common side reaction. Furthermore, the starting materials for the synthesis of a variety of photochromic nitrobenzyl pyridines are easily accessible. The half‐lives of the phototautomers of several new di‐ and trinitrobenzyl‐substituted pyridines were determined. Copyright © 2010 John Wiley & Sons, Ltd.
A study to determine the prevalence of postpartum depression among postnatal mothers at Venkatachalam Village, Nellore, Andhra Pradesh (2019)

Background: Mood disturbance represents the most frequent from of maternal psychiatric morbidity in the postpartum period. Maternal sadness affects approximately 50-80% of women in the puerperal period with about 20% of those women developing postpartum depression. Aim: The aim of the study was to assess the prevalence of postpartum depression among postnatal mothers. Objectives: To determine the prevalence of postpartum depression among postnatal mothers. 2. To associate the prevalence of postpartum depression among postnatal mothers with their selected socio demographic variables. Methodology: 100 postnatal mothers living in Venkatachalam Village, Nellore were selected by using Non-probability convenience sampling method. Results: Regarding the prevalence of postpartum depression, among 100 samples, 52(52%) had no depression, 30 (30%) had mild depression and 18 (18%) had moderate depression and no one had severe depression.
Pseudocholinesterase Deficiency: A Case Report and Literature Review (2012)

A 72-year-old male underwent neck dissection and parotidectomy with facial nerve preservation. Endotracheal intubation was facilitated with succinylcholine. Prolonged muscle paralysis which was first detected after failure to stimulate the facial nerve with electrocautery, lasted five hours. Laboratory tests indicated pseudocholinesterase (PChE) deficiency. A thyroidectomy one month later was performed uneventfully using rocuronium as a muscle relaxant. Literature review revealed a total of 40 PChE deficiency cases being reported since 1956.
Misdirected antibody responses against an N‐terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections (2011)

Rhinoviruses (RVs) are the primary cause of upper respiratory tract infections, generally known as the common cold. Moreover, RV infections can trigger severe exacerbations of asthma and chronic obstructive pulmonary disease (COPD). We expressed the 4 major RV capsid proteins, VP1-VP4, in Escherichia coli and used these proteins as well as recombinant and synthetic VP1 fragments to study and map antibody responses in RV-infected humans. VP1, which on infection binds to ICAM 1, was identified as a major target for the memory immune response, residing in the IgG1 subclass and IgA class. Interestingly, this response was mainly directed against an N-terminal 20 mer peptide in VP1, P1a, which becomes exposed on intact RV only when it docks to its receptor ICAM 1. Molecular modeling using the 3-dimensional RV capsid structures revealed that P1a was localized inside the capsid and outside the areas involved in receptor binding or RV neutralization. Our results suggest misdirection of antibody responses against a nonprotective epitope as a mechanism how RV escapes immunity and causes recurrent infections. Based on these findings, it may be possible to design vaccines against RV infections and RV-induced respiratory diseases.
Expression Pattern of Connexin Gene Products at the Early Developmental Stages of the Mouse Cardiovascular System (1997)

The synchronized contraction of myocytes in cardiac muscle requires the structural and functional integrity of the gap junctions present between these cells. Gap junctions are clusters of intercellular channels formed by transmembrane proteins of the connexin (Cx) family. Products of several Cx genes have been identified in the mammalian heart (eg, Cx45, Cx43, Cx40, and Cx37), and their expression was shown to be regulated during the development of the myocardium. Cx43, Cx40, and Cx45 are components of myocyte gap junctions, and it has also been demonstrated that Cx40 was expressed in the endothelial cells of the blood vessels. The aim of the present work was to investigate the expression and regulation of Cx40, Cx43, and Cx37 during the early stages of mouse heart maturation, between 8.5 days post coitum (dpc), when the first rhythmic contractions appear, and 14.5 dpc, when the four-chambered heart is almost completed. At 8.5 dpc, only the reverse-transcriptase polymerase chain reaction technique has allowed identification of Cx43, Cx40, and Cx37 gene transcripts in mouse heart, suggesting a very low activity level of these genes. From 9.5 dpc, all three transcripts became detectable in whole-mount in situ-hybridized embryos, and the most obvious result was the labeling of the vascular system with Cx40 and Cx37 anti-sense riboprobes. Cx40 and Cx37 gene products (transcript and/or protein) were demonstrated to be expressed in the vascular endothelial cells at all stages examined. By contrast, only Cx37 gene products were found in the endothelial cells of the endocardium. In heart, Cx37 was expressed exclusively in these cells, which rules out any direct involvement of this Cx in the propagation of electrical activity between myocytes and the synchronization of contractions. Between 9.5 and 11.5 dpc, Cx40 gene activation in myocytes was demonstrated to proceed according to a caudorostral gradient involving first the primitive atrium and the common ventricular chamber (9.5 dpc) and then the right ventricle (11.5 dpc). During this period of heart morphogenesis, there is clearly a temporary and asymmetrical regionalization of the Cx40 gene expression that is superimposed on the functional regionalization. In addition, comparison of Cx40 and Cx43 distribution at the above developmental stages has shown that these Cxs have overlapping (left ventricle) or complementary (atrial tissue and right ventricle) expression patterns.
Tunneling leakage in Ge preamorphized shallow junctions (1988)

CMOS shallow junctions with depths less than 0.2 mu m were fabricated using Ge preamorphization and rapid thermal annealing. A low bulk generation current (<1 nA/cm/sup 2/) for both poly-gated and Al gated diodes was obtained by placing the extended end-of-range defects inside the heavily doped junction. However, gated diode characterization shows a large tunneling current component in addition to the bulk generation current when the surface is accumulated by the gate. By comparing preamorphized with nonpreamorphized junctions for both poly-gated and Al gated diodes, it is concluded that the tunneling effect is due to the presence of the midgap states within or near the depletion region at the surface of the junction edge. These midgap states are a result of the crystal damage associated with Ge preamorphization. PISCES simulations of this tunneling current show good agreement with the experimental results, which show that gate-induced drain leakage in n/sup +/ poly-gate PMOS devices at high drain voltages is strongly dependent on removal of junction implantation damage.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>