gebhart/patrepeval huggingface dataset:
3-Hydroxyphthaloyl β-Lactoglobulin. IV. Antiviral Activity in the Mouse Model of Genital Herpesvirus Infection (1998)

The spread of sexually transmitted infections caused by herpes simplex virus type 2 (HSV-2) has continued unabated despite educational efforts generated in response to the human immunodeficiency virus (HIV) epidemic. Given the absence of effective vaccines, this indicates the need to develop prophylactic measures such as topical antiviral agents. Chemical modification of bovine beta-lactoglobulin (beta-LG), the major protein of whey, by hydroxyphthalic anhydride (3HP) led to the generation of a potent HIV-1 inhibitor designated 3HP-beta-LG. This agent was shown to also have antiviral activity against HSV-2 and HSV-1 in vitro. Recent studies indicate that 3HP-beta-LG binds to HSV-1 virions, which, at least in part, involves the viral glycoprotein gE. Here we show that 3HP-beta-LG inhibits HSV-2 infection in the mouse model of genital HSV-2 infection. Simultaneous exposure to HSV-2 and 3HP-beta-LG caused a significant decrease in the proportion of infected animals (27% virus shedding, 5% lesion development and 0% fatality for 3HP-beta-LG as compared to 80% shedding, 60% lesion development and 53% fatality in mice treated with PBS). The proportion of animals with HSV-2 infection after treatment with beta-LG was similar to that in the PBS-treated group. Pretreatment with 3HP-beta-LG formulated in a gel, which prolongs the presence of the agent in the vagina, also significantly reduced the proportion of HSV-2-infected mice (5% virus shedding, 5% lesion development and 0% fatality for 3HP-beta-LG as compared to 70% shedding, 60% lesion development and 40% fatality in vehicle-treated mice). These differences were significant (P < or = 0.0005, 0.002 and 0.008 for shedding, lesion development and fatality, respectively). Virus titres in the minority of mice that developed infection were similar to those in untreated mice. HSV-2 infection was not inhibited by treatment of an ongoing infection, indicating that 3HP-beta-LG interferes with the initial infection. These data suggest that 3HP-beta-LG may be an efficacious agent for preventing vaginal transmission of genital herpesvirus infections.
Design of Genetic Algorithm-based Parking System for an Autonomous Vehicle (2009)

A Genetic Algorithm (GA) is a kind of search techniques used to find exact or approximate solutions to optimization and searching problems. This paper discusses the design of a genetic algorithm-based intelligent parking system. This is a search strategy based on the model of evolution to solve the problem of parking systems. A genetic algorithm for an optimal solution is used to find a series of optimal angles of the moving vehicle at a parking space autonomously. This algorithm makes the planning simpler and the movement more effective. At last we present some simulation results.
Pore and solid diffusion models for fixed‐bed adsorbers (1974)

Abstract Most models for fixed bed adsorbers have used either the homogeneoussolid or pore diffusion model for the pellets. When the adsorption isotherm is linear, the two models can lead to identical breakthrough curves. The conditions for this equivalence are presented here. It is shown that one of the bulk flow factors that was included in the formulation of one pore diffusion model will be significant only for feedstreams containing a relatively high concentration of adsorbate. The prosity factor of the pore model is shown to be very important, especially as the porosity decreases. The importance of the two diffusional models with respect to the predicted breakthrough curves is demonstrated. For comparable beds, it is shown that the breakthrough curve based on the homogeneous model is delayed with respect to that based on the pore model at early times, regardless of the shape of the isotherm. Finally, the various possible solutions for an irreverisble isotherm are reviewed for each of the models, and a solution is presented for the general case of a pore model with an outside film resistance.
Cloning, sequencing, and overexpression in Escherichia coli of the alpha-D-glucose-1-phosphate cytidylyltransferase gene isolated from Yersinia pseudotuberculosis (1994)

A clone of Yersinia pseudotuberculosis DNA carrying the ascA gene was constructed, and the corresponding protein was successfully overexpressed in Escherichia coli. A protocol consisting of DEAE-cellulose and Sephadex G-100 column chromatography was developed and led to a nearly homogeneous purification of the ascA product. Initial characterization showed that the ascA-encoded protein is actually the alpha-D-glucose-1-phosphate cytidylyltransferase which catalyzes the first step of the biosynthesis of CDP-ascarylose (CDP-3,6-dideoxy-L-arabino-hexose), converting alpha-D-glucose-1-phosphate to CDP-D-glucose. In contrast to early studies suggesting that this enzyme was a monomeric protein of 111 kDa, the purified cytidylyltransferase from Y. pseudotuberculosis was found to consist of four identical subunits, each with a molecular mass of 29 kDa. This assignment is supported by the fact that the ascA gene, as a part of the ascarylose biosynthetic cluster, exhibits high sequence homology with other nucleotidylyltransferases, and its product shows high cytidylyltransferase activity. Subsequent amino acid comparison with other known nucleotidylyltransferases has allowed a definition of the important active-site residues within this essential catalyst. These comparisons have also afforded the inclusion of the cytidylyltransferase into the mechanistic convergence displayed by this fundamental class of enzyme.
Automating ECU Identification for Vehicle Security (2016)

The field of vehicular cybersecurity has received considerable media and research attention in the past few years. Given the increasingly connected aspect of consumer automobiles, along with the inherent danger of these machines, there has been a call for experienced security researchers to contribute towards the vehicle security domain. The proprietary nature of Controller Area Network (CAN) bus messages, however, creates a barrier of entry for those unfamiliar, due to the need to identify what the messages on a given vehicle's bus are broadcasting. This work aims to automate the process of correlating CAN bus messages with specific Electronic Control Unit (ECU) functions in a new vehicle, by creating a machine learning classifier that has been trained on a dataset of multiple vehicles from different manufacturers. The results show that accurate classification is possible, and that some ECUs that broadcast similar vehicle dynamics broadcast similar CAN messages.
The Electromechanical Response of Multilayered Piezoelectric Structures (2004)

The constitutive equations of multilayered piezoelectric structures are derived in a new form. In this form, the electromechanical coupling is presented as an additional stiffness matrix. This matrix is a true property of the piezoelectric structure and is independent of specific mechanical boundary conditions that may apply to the structure. A novel model of the electromechanical response of such structures is presented. This model accounts for the three-dimensional (3-D) kinematics of the structure deformation. Solution of example problems using the new model shows excellent agreement with full 3-D finite element simulations. These solutions are also compared to the results of previous two-dimensional (2-D) model approximations presented in literature, and the inaccuracies associated with these previous models are discussed.
Physician Recruitment Strategies for a Rural Hospital (2001)

St. Vincent Randolph Hospital, Winchester, Indiana James Full, FACHE, serves as CEO for St. Vincent Randolph Hospital in Indiana. Mr. Fuller has previously worked for Alliant Management Services in Louisville, Kentucky, and was the executive director for the Greater Randolph Community Health Plan in Winchester, Indiana. In these positions he has coordinated the merging of a rural hospital system with the St. Vincent Hospital System/Ascension Health; is coordinating the planning for construction of a new critical access hospital; and has activated specialty clinics, comprehensive wellness and occupational medical programs, and model rural health clinics and training centers to provide rural exposure for medical residents and students. Prior to these experiences, Mr. Full worked as a CEO for a Charter Medical Corporation Hospital in Lafayette, Indiana, and as administrator for the Veterans Administration in Colorado and Illinois. Mr. Full is a member of the National Association of Social Workers, the Indiana Hospital and Health Association Council on Rural Health, the Indiana Primary Care Association, and the Indiana Rural Health Association. He has received several awards for innovative program development and volunteers his time with Junior Achievement, Boy Scout Medical Explorers, Rotary International, and the Randolph County United Way. He has been a member of the American College of Healthcare Executives since 1985 and achieved Fellow status in 1999. This case study represents a part of his Fellow project and was voted one of the best case studies in 1999.
[Regional anticoagulation with sodium citrate: chronic utilization in the hemodialysis patient]. (1993)

We used an alternative technique for regional citrate anticoagulation (citrate 0.48 Mol; bicarbonate containing dialysate with Ca 1.75 Mmol) in three patients at chronic risk of bleeding and for a mean follow-up period of ten months. No bleeding recurrence was observed nor any complication related to citrate. Hemoglobin and hematocrit rose without transfusions. Serum bicarbonate increased during the first four months of treatment. No significant change was observed in serum calcium nor serum sodium concentration.
Clinical Evaluation of a Computer-controlled Pressure Support Mode (2000)

We have designed a computerized system providing closed-loop control of the level of pressure support ventilation (PSV). The system sets itself at the lowest level of PSV that maintains respiratory rate (RR), tidal volume (VT), and end-tidal CO(2) pressure (PET(CO(2))) within predetermined ranges defining acceptable ventilation (i.e., 12 < RR < 28 cycles/min, VT > 300 ml [> 250 if weight < 55 kg], and PET(CO(2)) < 55 mm Hg [< 65 mm Hg if chronic CO(2) retention]). Ten patients received computer-controlled (automatic) PSV and physician-controlled (standard) PSV, in random order, during 24 h for each mode. An estimation of occlusion pressure (P(0.1)) was recorded continuously. The average time spent with acceptable ventilation as previously defined was 66 +/- 24% of the total ventilation time with standard PSV versus 93 +/- 8% with automatic PSV (p < 0.05), whereas the level of PSV was similar during the two periods (17 +/- 4 cm H(2)O versus 19 +/- 6 cm H(2)O). The time spent with an estimated P(0.1) above 4 cm H(2)O was 34 +/- 35% of the standard PSV time versus only 11 +/- 17% of the automatic PSV time (p < 0.01). Automatic PSV increased the time spent within desired ventilation parameter ranges and apparently reduced periods of excessive workload.
Synthetic applications of 2‐chloro‐3‐formylquinoline (1987)

Abstract New examples of synthetic applications of 2‐chloro‐3‐formylquinoline, as evident from the novel and facile syntheses of 3‐aminoisooxazolo[5,4‐ b ]quinoline ( 4 ), 3‐hydroxyfuro[2,3‐ d ]theino[2,3‐ b ]quinoline‐2‐carboxamide (7) and 3‐hydroxymethyl‐2‐(3‐formyl)phenylquinoline ( 13 ), have been furnished.
Evidence for a second alpha 2-macroglobulin receptor. (1994)

alpha 2-Macroglobulin (alpha 2M)-methylamine binds to purified low density lipoprotein receptor-related protein (LRP), and it is assumed that LRP functions as the alpha 2M receptor in vivo. Binding of alpha 2M-methylamine to macrophage receptors elevates intracellular calcium ([Ca2+]i), inositol phosphates, and cyclic AMP. We have employed human alpha 2M-methylamine and recombinant receptor binding fragment (RBF) to study transduction mechanisms. Macrophages exposed to either ligand demonstrated a rapid rise in [Ca2+]i. Since the 39-kDa LRP/alpha 2M receptor-associated protein (RAP) blocks alpha 2M binding to LRP, we explored the effects of RAP upon signaling. Pretreatment of macrophages with RAP did not block the increase in [Ca2+]i elicited by alpha 2M-methylamine or RBF, suggesting a distinct binding site. RBF also elicited a transient 1.5-2.0-fold increase in inositol 1,4,5-triphosphate. In permeabilized macrophages, GTP gamma S and Gp-p(NH)p potentiated and sustained this inositol 1,4,5-triphosphate increase. Preincubation of permeabilized macrophages with GDP beta S abrogated the effects of GTP gamma S. Our results suggest that the signaling alpha 2M receptor is coupled to a pertussis toxin-insensitive G protein and possibly to a cholera toxin-sensitive G protein. We conclude that macrophages contain a second alpha 2M receptor that is G protein-coupled.
Automated Identification of Flying Insects by Analysis of Wingbeat Frequencies (1986)

A microcomputer-based instrument was designed to record and analyze flight movements of individual insects flying through a light beam. In an experiment with Aedes aegypti (L.) and A. triseriatus Say, species and sex of individuals were identified correctly with an accuracy of 84% using wingbeat frequency measurements. This technology may be useful in automated monitoring of flying insects in the field.
A review on Berberis species reported from Gilgit-Baltistan and Central Karakoram National Park, Pakistan. (2015)

Genus Berberis is medicinally, ecologically, commercially and ethnobotanically important yellow woody plants. There are two subspecies of Berberis pseudumbellata Parker found in Gilgit-Baltistan, however, since 1935 more than 33 researchers have reported fourteen (14) Berberis species and subspecies. Species found exhibit close morphological similarity. This review is compiled for the first time and will help researchers to overcome ongoing confusion during filed based discrimination. Berberis pseudumbellata Parker subsp. gilgitica is endemic to Gilgit-Baltistan and has become critically endangered.
Analysis of the phosphatidylinositol 3'-kinase signaling pathway in glioblastoma patients in vivo. (2003)

Deregulated signaling through the phosphatidylinositol 3'-kinase (PI3K) pathway is common in many types of cancer, including glioblastoma. Dissecting the molecular events associated with activation of this pathway in glioblastoma patients in vivo presents an important challenge that has implications for the development and clinical testing of PI3K pathway inhibitors. Using an immunohistochemical analysis applied to a tissue microarray, we performed hierarchical clustering and multidimensional scaling, as well as univariate and multivariate analyses, to dissect the PI3K pathway in vivo. We demonstrate that loss of the tumor suppressor protein PTEN, which antagonizes PI3K pathway activation, is highly correlated with activation of the main PI3K effector Akt in vivo. We also show that Akt activation is significantly correlated with phosphorylation of mammalian target of rapamycin (mTOR), the family of forkhead transcription factors (FOXO1, FOXO3a, and FOXO4), and S6, which are thought to promote its effects. Expression of the mutant epidermal growth factor receptor vIII is also tightly correlated with phosphorylation of these effectors, demonstrating an additional route to PI3K pathway activation in glioblastomas in vivo. These results provide the first dissection of the PI3K pathway in glioblastoma in vivo and suggest an approach to stratifying patients for targeted kinase inhibitor therapy.
Effect of environmental pH on the fermentation balance of Lactobacillus reuteri (1994)

The influence of environmental pH on the regulation of glucose catabolism by Lactobacillus reuteri was examined in anaerobic batch cultures. Under acidic conditions both glucose consumption and end‐products formation were low. Maximum biomass was reached at pH 5·0, with a specific growth rate of µ= 0·78 h ‐1 . The shift in pH values from 4.3 to 6.5 reflected an increase in glucose uptake as well as in the yield ( Y p/x ) of acetate, lactate and ethanol after 12 h of incubation. Ethanol was the major metabolite produced at all pH values assayed.
[The antagonistic properties of bacteria isolated from the digestive tract of female mink housed in the area of the Chernobyl Atomic Electric Power Station]. (1997)

Differences in species composition, number and level of antagonistic activity of bacteria isolated from the digestive tract of Chernobyl female minks of various age and with different immunological state have been established. Prevalence of anaerobes (bifidobacteria) and microaerophils (lactic acid bacteria) with the increase of microorganisms concentration along the channel: stomach, small and large intestine (10(7)-10(10)/g) was found in all the departments of digestive tract of minks. Among the identified lactic-acid bacteria Lactobacillus helveticus (10(7)-10(8)/g) prevailed in the stomach of the studied female minks, L. coryniformis (10(9)-10(10)/g) in the small intestine, L. casei (10(10)/g) in the large one. Antagonistic activity was most expressed in the strains of L. helveticus and L. casei, isolated from the younger (1.5 year-old) minks. Enterococcus faecalis isolated from the stomach of 1.5 year-old female minks was distinguished by the greatest antagonistic activity among identified enterococci. Strains of E. faecium isolated from the thin intestine of the young female minks (1.5 year-old) and from the large intestine of more nature animals (2.5 years) who received thymogen were characterized by the most expressed antibiosis among enterococci isolated bacteria a conclusion was made that the mechanisms of inhibitory effect of the studied microorganisms are underlied by not only their capacity to form organic acids but also by the capacity to produce antibiotic products.
A Monoclonal Antibody with Thyrotropin (TSH) Receptor Inverse Agonist and TSH Antagonist Activities Binds to the Receptor Hinge Region as Well as to the Leucine-Rich Domain (2009)

Monoclonal antibody CS-17 is a TSH receptor (TSHR) inverse agonist (suppresses constitutive activity) and a TSH antagonist. Elucidation of the CS-17 epitope will provide insight into TSHR structure and function. Present information on its epitope conflicts with recent data regarding another TSHR inverse agonist antibody. To characterize further the CS-17 epitope, we exploited the observation that CS-17 does not recognize a chimeric receptor with TSHR hinge region residues 261-289 replaced with homologous rat LH receptor residues (13 mismatches). We generated individual and double TSHR mutations corresponding to these mismatches. On flow cytometry, only T273L/R274V reduced CS-17 recognition. No mutation affected TSH-stimulated cAMP generation. Because the immunogen for CS-17 generation was highly glycosylated, we also investigated whether the glycan moiety at N198, topologically adjacent to Y195 (a previously identified epitopic component), could contribute to the CS-17 epitope. Elimination of this N-linked glycan (mutations of N198 and T200) abrogated CS-17 binding without altering TSH responsiveness. However, studies with tunicamycin suggested that these mutations affected CS-17 binding by altering the polypeptide backbone rather than eliminating the glycan moiety. TSHR residues N198 and T200, like Y195, are on the convex facet of the leucine-rich domain. In summary, the present data indicate that the discontinuous epitope of CS-17, a TSHR inverse agonist and TSH antagonist, includes a component in the hinge region as well as the convex surface of the TSHR leucine-rich domain. These findings expand our present concept of glycoprotein hormone binding and function.
Using noise for controlled disassembly of nanoscale gold wires (2006)

Gold wires 90 nm wide were fabricated on a 100 nm thin silicon nitride membrane using electron beam lithography. Application of a bias voltage across the wires caused them to break, due to resistive heating and electromigration, and produced a nanoscale gap in their structure. Placing the gold wires on a thin silicon nitride membrane allowed us to image the wires with transmission electron microscopy as they proceeded through the breaking process with a very high resolution. The images revealed extensive rearrangement of the metal grains and the exact shape of the final gap. A voltage-ramp algorithm using the effective conductance of the wires as the feedback parameter was developed to reproducibly generate 2 nm gaps in the wire structure. We observed that the measured relative current noise increased more than two orders of magnitude more than the resistance in the first stage of the disassembly process. The large change of the current noise makes it an attractive candidate as a parameter to be used in the control feedback loop to break the wires. Initial results for using noise to control the disassembly process are presented.
A Kinetic Study of the ortho-Claisen Rearrangement<sup>1</sup> (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Kinetic Study of the ortho-Claisen Rearrangement1Harlan L. Goering and Robert R. JacobsonCite this: J. Am. Chem. Soc. 1958, 80, 13, 3277–3285Publication Date (Print):July 1, 1958Publication History Published online1 May 2002Published inissue 1 July 1958https://pubs.acs.org/doi/10.1021/ja01546a024https://doi.org/10.1021/ja01546a024research-articleACS PublicationsRequest reuse permissionsArticle Views978Altmetric-Citations116LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts