gebhart/patrepeval huggingface dataset:
Repeated Gene Transfer of Naked Prostacyclin Synthase Plasmid into Skeletal Muscles Attenuates Monocrotaline-Induced Pulmonary Hypertension and Prolongs Survival in Rats (2004)

A safer, less invasive method for repeated transgene administration is desirable for clinical application of gene therapy targeting chronic diseases, including pulmonary hypertension (PH). Thus, effects of prostaglandin I2 (prostacyclin) synthase (PGIS) gene transfer by the naked DNA method into skeletal muscle were investigated in monocrotaline (MCT)-induced PH rats. A single injection of rat PGIS cDNA-encoding plasmid into thigh muscle 3 days after bupivacaine pretreatment transiently increased muscle PGIS protein expression and muscle and serum levels of a stable prostacyclin metabolite (6-keto-prostaglandin F1). The muscle 6-keto-prostaglandin F1 level peaked on day 2 but was still elevated on day 7; prostacyclin selectively increased lung cyclic AMP levels as compared with liver and kidney. MCT induced a marked rise in right ventricular (RV) systolic pressure, pulmonary arterial wall thickening, and RV hypertrophy. Repeated PGIS gene transfer every week lowered RV systolic pressure and ameliorated RV and pulmonary artery remodeling in MCT-induced PH rats. Furthermore, repeated PGIS gene transfer significantly improved the survival rate of MCT-induced PH rats. In conclusion, repeated PGIS gene transfer into skeletal muscle not only attenuated the development of PH and cardiovascular remodeling but also improved the prognosis for MCT-induced PH rats. This study may provide insight into a new treatment strategy for PH.
Test bed for time jitter studies of laser-triggered gas-discharge switches (2011)

Laser-triggering of high-voltage spark gaps is a reliable method of obtaining low switching time jitter. To further the advantage of laser triggering compared to other trigger methods an optical fibre is used to guide the laser pulse into the switch. This paper presents a test bed for the study of laser-triggered spark gaps. An initial test series using a 1064-nm, 5-ns laser pulse shows that a time jitter down to, or even below, one nanosecond is readily achieved for a normalised voltage down to 0.6, the threshold laser intensity required to trigger the spark gap being below 2 TW/m <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> .
Market Segmentation Within Consolidated E-Markets: A Generalized Combinatorial Auction Approach (2006)

We analyze an e-market design that allows multiple market segments to be served simultaneously with a single generalized combinatorial auction. The mechanism uses rule-based bids designed to accommodate various kinds of bidders, such as those more sensitive to price or those more restricted in their requirements. We demonstrate experimentally—using agent-based simulation of the actual market for television advertising slots—that the rule-based approach effectively handles the wide range of market segments, while maintaining buyer and seller surplus and efficiently allocating goods.
The Dielectric Constant of Water at High Temperatures and in Equilibrium with its Vapor (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Dielectric Constant of Water at High Temperatures and in Equilibrium with its VaporG. C. Akerlof and H. I. OshryCite this: J. Am. Chem. Soc. 1950, 72, 7, 2844–2847Publication Date (Print):July 1, 1950Publication History Published online1 May 2002Published inissue 1 July 1950https://doi.org/10.1021/ja01163a006RIGHTS & PERMISSIONSArticle Views919Altmetric-Citations126LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (439 KB) Get e-Alerts Get e-Alerts
Radiosynthesis and preliminary evaluation of 11C-levetiracetam for PET imaging of SV2A expression (2014)

1795 Objectives Synaptic vesicle protein 2A (SV2A) has been identified as an interesting therapeutic and imaging target for epilepsy and other neurologic disorders. Herein, we report the first radiosynthesis of 11C-levetiracetam (11C-LEV) and its preliminary evaluation for PET imaging of SV2A expression. Methods The multi-step preparation of 11C-LEV was carried out by a one-pot radiosynthesis. Briefly, propionaldehyde was converted to propan-1-imine in situ as the labeling precursor. Without further separation, the imine was reacted with [11C]HCN to form 11C-aminonitrile, which was then reacted with 4-chlorobutyryl chloride under a basic condition. The crude was hydrolyzed to yield 11C-LEV, which was separated by chiral HPLC. After in vitro evaluation of 11C-LEV, a 60 min dynamic microPET/CT imaging was performed for assessment of SV2A expression in mice. Results A new tracer for PET imaging of SV2A expression, 11C-LEV, was designed and synthesized. The radiochemical yield (decay corrected) of 11C-LEV was 8.3 ± 1.6% (n = 8) based on [11C]HCN radioactivity. Both of the radiochemical and enantiomeric purities of 11C-LEV were > 98%. The total radiosynthesis time was 50 ± 5 min from the end of bombardment to the finish of HPLC purification. After 90 min of incubation with the formulation solution, > 98% of 11C-LEV remained stable. The log P was determined to be -0.60. The dynamic microPET/CT imaging in mice showed high initial uptake of the tracer in most organs, followed by gradual washout, but brain uptake was accumulated at 60 min. The imaging specificity of SV2A expression was subsequently validated through a blocking experiment with cold levetiracetam. Conclusions The radiosysthesis of 11C-LEV was first designed and achieved by one-pot multi-step reaction. Preliminary evaluations of 11C-LEV demonstrated its potential for specific PET imaging of SV2A expression.
Sonographic scoring index for prenatal detection of chromosomal abnormalities (1992)

Current indications for cytogenetic evaluation leave the majority of Down syndrome fetuses undetected. Using advanced maternal age and low maternal serum alpha-fetoprotein (AFP) levels as criteria, only 40% of fetuses with Down syndrome (trisomy 21) are identified (positive predictive value, 0.4% to 1%). We evaluate the sonographically detectable physical features of second trimester fetuses to determine whether these features are more sensitive and specific than maternal age for detecting fetuses with abnormal karyotypes. From March 1, 1990, to September 1, 1991, more than 5,000 fetuses between 14 and 20 weeks of development were referred for genetic amniocentesis because of advanced maternal age or abnormal AFP levels. Forty-three of these 5,000 fetuses were later found to have autosomal trisomies by karyotype (32 with trisomy 21, nine with trisomy 18, and two with trisomy 13). A sample of 588 consecutive normal fetuses from the total of more than 5,000 amniocenteses performed during this period of time was used as our control group for statistical analysis. The sonographic features of these 588 normal second trimester fetuses and the 43 trisomic fetuses recorded prospectively prior to knowledge of the karyotype were evaluated statistically. The femur and humerus lengths, nuchal fold, renal pelvic dimension, and major structural defects were compared in the normal and trisomic fetuses. On the basis of our results, a weighted sonographic score was developed to optimize the detection of fetuses at risk for aneuploidy. Using our previously published formulas and criteria for a short femur and humerus, 17/32 (53%) fetuses with Down syndrome and 23/588 (3.9%) of the normal fetuses were identified. Twenty two of 32 Down syndrome fetuses (69%) and 2/588 (0.34%) of normals had a nuchal fold > or = 6 mm, and 11 of 32 Down syndrome fetuses and all those with trisomies 18 and 13 had a major anomaly detected sonographically. The following scoring system was developed for the detection of aneuploidy: nuchal fold = 2, major structural defect = 2, and short femur, short humerus, and pyelectasis = 1 each. Selecting fetuses with a score of > or = 2 would identify 26/32 (81%) Down syndrome fetuses, and 9/9 (100%) and 2/2 (100%) fetuses with trisomies 18 and 13 respectively, but only 26/588 (4.4%) of the normal fetuses. Using the sonographic score of 2 results in a positive predictive value for a 1/250 risk group of 6.87% for identifying Down syndrome fetuses and 7.25% for all three trisomies.(ABSTRACT TRUNCATED AT 400 WORDS)
Experimental Vertebroplasty Using Osteoconductive Granular Material (2000)

These results demonstrate the osteoconductivity of coral in granular form for vertebral filling. Interestingly, interconnectivity between adjacent bone trabeculae and newly formed bone was restored; however, its mechanical significance remains to be determined. Further investigations are needed to evaluate the efficacy of coral in osteopenic animals and in relieving pain.
Newton road bridge (1974)

This paper describes the main features of the Newton Road bridge, which is part of the Auckland urban motorway system, its architectural form, seismic behaviour, and some of the more interesting problems arising out of the continuous prestressed concrete box-girder design.
Pattern specific optical models (2008)

Computational efficiency of the models used in optical proximity correction (OPC) continues to be important in photolithography employing resolution enhancement. Improved hardware and model efficiency can mitigate the increase in run time resulting from the rise in modeling complexity necessary to meet advanced semiconductor process requirements. The current approach for fast image calculations in OPC utilizes the Hopkins formulation of the imaging equations combined with the sum-of-coherent-systems (SOCS) approximation. In this paper, approaches to identify the SOCS terms, which can be neglected in an OPC model applied to a given layout, are explored. One approach is quantified in terms of computational efficiency and model accuracy. The pattern-specific computational efficiency improvement ranges from 7% to 55% for six different patterning conditions used in 193nm immersion lithography at the 45nm semiconductor technology node.
Cell Surface Localization of Tissue Transglutaminase Is Dependent on a Fibronectin-binding Site in Its N-terminal β-Sandwich Domain (1999)

Increasing evidence indicates that tissue transglutaminase (tTG) plays a role in the assembly and remodeling of extracellular matrices and promotes cell adhesion. Using an inducible system we have previously shown that tTG associates with the extracellular matrix deposited by stably transfected 3T3 fibroblasts overexpressing the enzyme. We now show by confocal microscopy that tTG colocalizes with pericellular fibronectin in these cells, and by immunogold electron microscopy that the two proteins are found in clusters at the cell surface. Expression vectors encoding the full-length tTG or a N-terminal truncated tTG lacking the proposed fibronectin-binding site (fused to the bacterial reporter enzyme beta-galactosidase) were generated to characterize the role of fibronectin in sequestration of tTG in the pericellular matrix. Enzyme-linked immunosorbent assay style procedures using extracts of transiently transfected COS-7 cells and immobilized fibronectin showed that the truncation abolished fibronectin binding. Similarly, the association of tTG with the pericellular matrix of cells in suspension or with the extracellular matrix deposited by cell monolayers was prevented by the truncation. These results demonstrate that tTG binds to the pericellular fibronectin coat of cells via its N-terminal beta-sandwich domain and that this interaction is crucial for cell surface association of tTG.
Fiber-handling robot and optical connection mechanisms for automatic cross-connection of multiple optical connectors (2013)

Conventionally, in intelligent buildings in a metropolitan area network and in small-scale facilities in the optical access network, optical connectors are joined manually using an optical connection board and a patch panel. In this manual connection approach, mistakes occur due to discrepancies between the actual physical settings of the connections and their management because these processes are independent. Moreover, manual cross-connection is time-consuming and expensive because maintenance personnel must be dispatched to remote places to correct mistakes. We have developed a fiber-handling robot and optical connection mechanisms for automatic cross-connection of multiple optical connectors, which are the key elements of automatic optical fiber cross-connect equipment. We evaluate the performance of the equipment, such as its optical characteristics and environmental specifications. We also devise new optical connection mechanisms that enable the automated optical fiber cross-connect module to handle and connect angled physical contact (APC) optical connector plugs. We evaluate the performance of the equipment, such as its optical characteristics. The evaluation results confirm that the automated optical fiber cross-connect equipment can connect APC connectors with low loss and high return loss, indicating that the automated optical fiber cross-connect equipment is suitable for practical use in intelligent buildings and optical access networks.
A reciprocity approach for calculating radiation patterns of arbitrarily shaped microstrip antennas mounted on circularly cylindrical platforms (2003)

The paper presents a convenient and efficient approach, based on the reciprocity theorem, for calculating the radiation patterns of arbitrarily shaped microstrip antennas with dielectric substrate and superstrate layers mounted on circularly cylindrical platforms. A detailed theoretical development followed by examples with numerical and graphical results illustrate the versatility of the technique. The reciprocity approach presented is very flexible and may be used in conjunction with any of the commonly employed computational electromagnetics modeling approaches such as the method of moments, finite-element methods, and finite-difference time-domain techniques.
Top‐down characterization of proteins and drug‐protein complexes using nanoelectrospray tandem mass spectrometry (2005)

We report a 'top-down' approach for characterization of proteins, and identification of binding sites in protein-drug complexes using nanoelectrospray ionization hybrid quadrupole time-of-flight tandem mass spectrometry (nanoESI-MS/MS). The efficiency of direct fragmentation of intact protein ions and the feasibility of this method were initially demonstrated using several well-characterized proteins with different molecular weights including metallothionein (6126 Da), cytochrome c (horse, 12360 Da), myoglobin (horse, 16592 Da), and hemoglobin (human, 64453 Da). Simply varying collision energy without enzyme digestion and gel or LC separation generated a range of peptide fragments of these proteins. Over 80% of these peptide ions matched those in the SWISS-PROT database with mass accuracy of 8 to 32 ppm with external calibration. This technique was further applied to fragment a cisplatin-metallothionein complex to identify the binding sites, demonstrating a potential application in the study of drug-protein binding.
Deep Integration of Navigation Solution and Signal Processing (2005)

The paper presents an overview on techniques to aid signal processing in a GNSS receiver by feeding back information from the navigation solution. Its main focus lies on the implementation of a deep coupling scheme, similar to a vector DLL (VDLL), resp. vector FLL (VFLL). As receiver platform the Institute of Geodesy and Navigation Experimental Software Receiver (ipexSR) was used. The paper discusses the deep coupling concept at system level and presents results from a test run where the vector loop is compared to a conventional DLL/FLL implementation. The test run is performed by driving with a car through a university campus and represents a low visibility scenario. It demonstrates the three main benefits of the vector loop: increased availability, realistic Doppler/pseudorange accuracy assessment from signal processing and less computational load in the software receiver.
Strip test for bedside detection of interleukin-6 in cervical secretions is predictive for impending preterm delivery. (2001)

Inflammatory cytokines in amniotic fluid are markers of prematurity which could characterize preterm labour of infectious origin. To avoid amniocentesis, we analyzed IL-6, IL-8, IL-10, and IL-13 by RT-PCR in cervical secretions (CS) of 307 women with preterm labour. IL-6 was detected in 26.3% patients who delivered at less than 34 weeks (specificity: 95.8%). In addition, IL-6 was associated with delivery within 7 days (specificity: 91.6%). To render the detection more rapid and cheaper, a strip test was designed and evaluated comparatively with RT-PCR in 76 women. This bedside strip test was twice more sensitive than RT-PCR, with little decrease in specificity.
Genetic and Biochemical Studies on Yeast Strains Able to Utilize Dextrins (1978)

AbstractMajor carbohydrates in brewer's wort are sucrose, glucose, fructose, maltose, maltotriose and dextrin material. Normally, strains of ale (Saccharomyces cerevisiae) and lager (S. uvarum [carlsbergensis]) yeast can use only the sugars of hexose units 1–3, but some Saccharomyces strains use dextrin as sole carbon source for growth and fermentation; this is possible because an extracellular dextrinase or permease enzyme is excreted. Segregants of an S. diastaticus strain have at least one gene coding for dextrinase production. This gene (DEX 1) is on the right arm of chromosome III distal from the centromere. The enzyme coded by this gene hydrolyzes dextrin to glucose units and is considered to be amyloglucosidase. A nonbrewing Saccharomyces sp. haploid with ability to use dextrin as sole carbon source also has been found. The gene coding for this ability is unlinked (ie, in chromosome location) to DEX 1. Of the longer chain sugars, this system uses only maltotetraose and, to a lesser extent, maltopentose; it is regarded as a permease. The permease gene facilitates transport of maltotetraose and maltopentose across the cell membrane into the cytoplasm where α-glucosidase hydrolyzes these sugars to glucose. A MAL gene must "turn on" the maltose utilization system before a culture containing the permease gene can use maltotetraose and maltopentose.KeywordsBrewingDextrinDextrinaseFermentationGeneticsYeast
A 24 GHz band FM-CW radar system for detecting closed multiple targets with small displacement (2015)

In this paper, detection method for 24 GHz band FM-CW radar system under closed multiple targets with small displacements environment is proposed, and its performances are analyzed. The proposed detection method was used a tunable FIR filter for signal processing, and the distance and small displacement can be separated for each target correctly from the received signal including all of signals from the targets at the FM-CW radar. Computer simulations were carried out for evaluating the proposed detection method for FM-CW radar and analyzing the performance according to the parameters under closed multiple targets environment. The results show that the proposed detection method can detect both the distance and small displacement correctly under closed multiple targets environment.
Utilization of a cationic ligand for the intercalation of catalytically active rhodium complexes in swelling, layer-lattice silicates (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUtilization of a cationic ligand for the intercalation of catalytically active rhodium complexes in swelling, layer-lattice silicatesW. H. Quayle and Thomas J. PinnavaiaCite this: Inorg. Chem. 1979, 18, 10, 2840–2847Publication Date (Print):October 1, 1979Publication History Published online1 May 2002Published inissue 1 October 1979https://pubs.acs.org/doi/10.1021/ic50200a042https://doi.org/10.1021/ic50200a042research-articleACS PublicationsRequest reuse permissionsArticle Views80Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The inflammatory cytokine, <scp>GM</scp>‐<scp>CSF</scp>, alters the developmental outcome of murine dendritic cells (2012)

Fms-like tyrosine kinase 3 ligand (Flt3L) is a major cytokine that drives development of dendritic cells (DCs) under steady state, whereas GM-CSF becomes a prominent influence on differentiation during inflammation. The influence GM-CSF exerts on Flt3L-induced DC development has not been thoroughly examined. Here, we report that GM-CSF alters Flt3L-induced DC development. When BM cells were cultured with both Flt3L and GM-CSF, few CD8⁺ equivalent DCs or plasmacytoid DCs developed compared to cultures supplemented with Flt3L alone. The disappearance of these two cell subsets in GM-CSF + Flt3L culture was not a result of simple inhibition of their development, but a diversion of the original differentiation trajectory to form a new cell population. As a consequence, both DC progeny and their functions were altered. The effect of GM-CSF on DC subset development was confirmed in vivo. First, the CD8⁺ DC numbers were increased under GM-CSF deficiency (when either GM-CSF or its receptor was ablated). Second, this population was decreased under GM-CSF hyperexpression (by transgenesis or by Listeria infection). Our finding that GM-CSF dominantly changes the regulation of DC development in vitro and in vivo has important implications for inflammatory diseases or GM-CSF therapy.