gebhart/patrepeval huggingface dataset:
Escherichia coli exports previously folded and biotinated protein domains (1991)

Biotination of proteins is a post-translational modification that requires a folded acceptor domain. We previously showed that an acceptor domain fused to the carboxyl terminus of several cytosolic proteins results in biotinated fusion proteins in vivo. We now show that proteins encoded by translational gene fusions of two periplasmic proteins, alkaline phosphatase and TEM beta-lactamase, to carboxyl-terminal biotin-accepting sequences are biotinated and exported by Escherichia coli. Expression of the alkaline phosphatase fusion protein in wild type strains resulted in inefficient biotination of the fusion product. This result was due to the rapid export of the acceptor protein before biotination could occur since a very large increase in biotinated fusion protein levels was observed in strains lacking the SecB chaperone protein. The beta-lactamase fusion protein was biotinated but was only stable in strains lacking the DegP periplasmic protease. Both biotinated fusion proteins accumulated in the culture medium in strains possessing defective outer membranes. These results indicate that the export machinery can accommodate both a post-translational modification and a protein domain previously folded into its mature conformation in vivo.
LSI yields high reliability: Cost and size of telephone-interface equipment shrinks, while IC designs displace electromechanical versions (1979)

Describes how cost and size telephone-interface equipment shrinks, as integrated circuit designs displace electromechanical versions.
Isolation and structure-activity relationship of some amylostatins (F-1b fraction) produced by streptomyces diastaticus subsp. amylostaticus No.9410. (1982)

Six amylostatins were isolated from the jS-amylase hydrolyzate of crude amylostatins (F-lb fraction) produced by Streptomyces diastaticus subsp. amylostaticus No. 9410.
Modifications to the sliding-window kernel RLS algorithm for time-varying nonlinear systems: Online resizing of the kernel matrix (2009)

A kernel-based recursive least-squares algorithm that implements a fixed size ldquosliding-windowrdquo technique has been recently proposed for fast adaptive nonlinear filtering applications. We propose a methodology of resizing the kernel matrix to assist in system identification of time-varying nonlinear systems. To be applicable in practice, the modified algorithm must preserve its ability to operate online. Given a bound on the maximum kernel matrix size, we define the set of all obtainable sizes as the resizing range. We then propose a simple online technique that resizes the kernel matrix within the resizing range. The modified algorithm is applied to the nonlinear system identification problem that was used to evaluate the original algorithm. Results show that an increase in performance is achieved without increasing the original algorithm's computation time.
Protein S–S bridge reduction: a Raman and computational study of lysozyme interaction with TCEP (2009)

The role of protein structure in the reactivity of the four disulfide (S-S) bridges of lysozyme was studied using Raman spectroscopy and molecular modelling. The experimental kinetics of S-S bridge reduction by tris-2-carboxyethyl phosphine (TCEP) was obtained by monitoring the protein S-S Raman bands. The kinetics are heterogeneous and were fitted using two apparent reaction rate constants. Kinetic measurements performed at different pH values indicate only moderate charge effects. The two intrinsic reaction rate constants derived for the neutral TCEP species were 0.45 and 0.052 mol(-1) s(-1), respectively. The molecular dynamics simulation of the reactants encounter shows that the accessibility of the lysozyme S-S bridges by TCEP decreases in the following order: cys30-cys115 > cys6-cys127 > cys64-cys80 > cys76-cys94. This simulation also illustrates the reaction mechanism which consists of a local unfolding followed by the reduction of the exposed S-S bridge. The Gibbs free energy for local unfolding was evaluated by comparing the actual reaction rate constant with that of a model system containing a fully exposed S-S bridge (oxidized glutathione). These values corresponding to the fast- and slow-reaction rate-constants were 8.5 and 13.8 kJ mol(-1), respectively. On the other hand, Raman measurements, as well as the molecular dynamics simulations, strongly suggest that the protein global unfolding following S-S bridge cleavage has only limited effects in stabilizing the reaction products.
Efficient memory shadowing for 64-bit architectures (2010)

Shadow memory is used by dynamic program analysis tools to store metadata for tracking properties of application memory. The efficiency of mapping between application memory and shadow memory has substantial impact on the overall performance of such analysis tools. However, traditional memory mapping schemes that work well on 32-bit architectures cannot easily port to 64-bit architectures due to the much larger 64-bit address space.
Reviews Transactions of the Cambridge Bibliographical Society. Vol. II, Part III (1956). Bowes & Bowes, London. Annual subscription: 21 <i>s</i> . Price 25 <i>s</i> . to non-members. (1957)

Journal Article Reviews Get access Transactions of the Cambridge Bibliographical Society. Vol. II, Part III ( 1956). Bowes & Bowes, London. Annual subscription: 21s. Price 25s. to non-members. J. C. T. O. J. C. T. O. Search for other works by this author on: Oxford Academic Google Scholar The Library, Volume s5-XII, Issue 3, September 1957, Page 219, https://doi.org/10.1093/library/s5-XII.3.219 Published: 01 September 1957
Expression changes of notch1 signaling and notch1 signaling-related miRNA in the hippocampus after traumatic brain injury (2011)

Objective To observe the expression of notch1-related miRNA, notch1 and nestin,and explore the regulatory mechanism of neural stem cells proliferation in hippocampus of mice after traumatic brain injury (TBI). Methods Mice were suffered closed head injury, and then sacrificed at 4th h,1st day and 3rd day post-injury. Real-time polymerase chain reaction (PCR) was used to detect the expression levels of mir-326, mir-34a and notch1 mRNA in mice hippocampus at 4th h, 1st day and 3rd day post TBI. Immunofluorescence was conducted to determine protein expression levels of notch1 and nestin in mice hippocampus at 3rd day post TBI. Results ( 1 ) Relative to sham group, the levels of mir-326 and mir-34a at 4th h, 1st day and 3rd day after TBI in the hippocampus were respectively (0. 36 ± 0. 16),(0. 16±0.04), (0.36±0. 17) and (0.48±0. 15), (0.50±0.04), (0.25 ±0. 14) fold (P＜0.01);(2) Relative to sham group, the levels of notch1 mRNA at 4th h, 1st day and 3rd day post TBI injury in the hippocampus were respectively (1.77 ±0. 17), (2.55 ±0.48) and (2.44±0.58) fold (P＜0.05).Notch1 + cells in the DG at 3rd day post TBI were significantly increased compared to sham group ( 18.20± 3.56 vs 0. 40 ±0. 55 ,P ＜0. 01 ); (3) Nestin + cells in the DG at 3rd day post TBI were increased apparently compared to sham group (21.80 ± 5. 07 vs 0.80 ± 0. 45, P ＜ 0. 01 ). Conclusion mir-326 and mir-34a may play a key role in trauma-induced neural stem cells proliferation in hippocampus in vivo by targeting notch1 signaling.

Key words: 
Traumatic brain injury;  Hippocampus;  Neural stem cells;  Proliferation
High-yield processing and single-mode operation of passive antiguide region vertical-cavity lasers (1997)

We report the first continuous-wave (CW) operation single-mode, low-threshold, passive antiguide region (PAR) vertical-cavity surface-emitting laser (VCSEL) using organometallic chemical vapor deposition (OMCVD) for the regrowth of the PAR structure. With improved laser wafer design, fabrication process and regrowth design, a stable single fundamental mode at high currents has been achieved experimentally for laser aperture as large as 16 /spl mu/m diameter. Single-mode power of 1.7 mW is achieved with more than 20-dB high-order mode suppression. The lasers show a more than 99% yield with high uniformity. Isolation etch and wet-thermal oxidation are used to study and eliminate the finite-leakage current in the PAR VCSEL's. Using multimode rate equations in conjunction with beam propagation method, single-mode lasing criteria is derived for PAR VCSEL. PAR VCSEL is shown to be a very efficient structure to meet the single-mode lasing condition.
79 GHz Slot Antennas Based on Substrate Integrated Waveguides (SIW) in a Flexible Printed Circuit Board (2009)

The design, fabrication and characterization of 79 GHz slot antennas based on substrate integrated waveguides (SIW) are presented in this paper. All the prototypes are fabricated in a polyimide flex foil using printed circuit board (PCB) fabrication processes. A novel concept is used to minimize the leakage losses of the SIWs at millimeter wave frequencies. Different losses in the SIWs are analyzed. SIW-based single slot antenna, longitudinal and four-by-four slot array antennas are numerically and experimentally studied. Measurements of the antennas show approximately 4.7%, 5.4% and 10.7% impedance bandwidth (S <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">11</sub> =-10 dB) with 2.8 dBi, 6.0 dBi and 11.0 dBi maximum antenna gain around 79 GHz, respectively. The measured results are in good agreement with the numerical simulations.
Enterprise architecture and object-oriented development (2002)

The paper describes the benefits of using an enterprise information architecture to reusable software components-specifically objects. The paper also describes Enterprise Engineering-a rigorous and repeatable methodology that allows organizations to realize the full potential of component development. Enterprise Engineering features technology independence and tightly defined links between business requirements, logical component models, and physical component designs.
An Electropolishing Technique for Germanium and Silicon (1963)

An electrochemical technique has been developed for polishing germanium and silicon to yield smooth and flat semiconductor surfaces. This technique is based on the anodic dissolution of a semiconductor whose surface is close to a moving cathode. The average surface roughness of electropolished semicon‐ductors was found to be ±25Aå and the deviation from flatness was typically 1 µ/cm.
Coronary Artery Aneurysm Induced by Kawasaki Disease in Children Show Features Typical Senescence (2007)

Histological features of senescence and active remodeling gene expression show that the KD aneurysm is not a silent vasculitis terminal. The future fate of KD aneurysms, including atherosclerosis, should be monitored carefully.
Is there an ideal facelift procedure? (2007)

This article provides surgeons performing facial rejuvenation surgery with a reference for evaluating which surgical techniques to add into their repertoires and a consensus of the experts as to the indications for each procedure, from major operative procedures to techniques that can be performed in the office.
ABC of prostate cancer (2012)

Contributors vii Foreword ix 1 Applied Anatomy of the Prostate 1 Prasanna Sooriakumaran Karl-Dietrich Sievert Abhishek Srivastava and Ashutosh Tewari 2 Pathology of Prostate Cancer 5 Murali Varma and Ashish Chandra 3 Biology of Prostate Cancer 8 R. William G. Watson and John M. Fitzpatrick 4 Prostate Cancer Diagnosis 11 Robin Weston Anthony J. Costello and Declan G. Murphy 5 Imaging of Prostate Cancer 14 James Halls and Uday Patel 6 Screening for Prostate Cancer 18 Richard J. Bryant and Freddie C. Hamdy 7 Active Surveillance 22 Judith Dockray Pardeep Kumar Lars Holmberg and Gordon Muir 8 Open Radical Prostatectomy 25 Wim van Haute and Hein van Poppel 9 Laparoscopic Radical Prostatectomy 29 Pardeep Kumar and Declan Cahill 10 Robotic Radical Prostatectomy 33 Ben Challacombe and Jim Peabody 11 Prostate Brachytherapy 37 Peter Acher and Rick Popert 12 HIFU 40 Louise Dickinson Hashim U. Ahmed and Mark Emberton 13 Cryotherapy for Prostate Cancer 44 Alastair Henderson and John Davies 14 Advances in External Beam Radiotherapy 48 David Landau Nadia Walsh and Asad Qureshi 15 Recent Advances in Hormonal Therapy 51 Heather Payne Reena Davda and Mark R. Feneley 16 Management of Castration-Resistant Prostate Cancer 54 Roger S. Kirby Prokar Dasgupta and John M. Fitzpatrick 17 Immunology of Prostate Cancer 57 Oussama Elhage Christine Galustian Richard Smith and Prokar Dasgupta 18 New Approaches to Prostate Cancer 63 Megan S. Schober Roger S. Kirby and Prokar Dasgupta Index 67
Nasopharyngeal airway stenting devices for obstructive sleep apnoea: A systematic review and meta-analysis (2014)

Some studies have demonstrated limited effectiveness and low tolerability of nasopharyngeal airway stenting devices, while other studies have shown a significant benefit in treating obstructive sleep apnoea, with a high level of patient acceptance. Nasal trumpets have been successful in decreasing airway obstruction in the short term.
The Modeling Process with System Dynamics (2007)

This chapter contains sections titled: System Dynamics Background General System Behaviors Modeling Overview Problem Definition Model Conceptualization Model Formulation and Construction Simulation Model Assessment Policy Analysis Continuous Model Improvement Software Metrics Considerations Project Management Considerations Modeling Tools Major References Chapter 2 Summary Exercises
Efficient crosstalk estimation (2003)

With the reducing distances between wires in deep sub-micron technologies, coupling capacitances are becoming significant as their magnitude becomes comparable to the area capacitance and fringing capacitance of a wire. This causes an increasing susceptibility to failure due to inadvertent noise, and leads to a requirement for accurate noise estimation. An incorrect estimation of the noise could lead either to circuit malfunction in the case of under-estimation, or to wasted design resources due to overestimation. This paper presents a new time-efficient method for the precise estimation of crosstalk noise. While existing fast noise estimation metrics may overestimate the coupling noise by several orders of magnitude, the proposed metric computes the coupling noise with a good accuracy as compared to SPICE.
Attraction of zebrafish, <i>Brachydanio rerio</i>, to alanine and its suppression by copper (1990)

Preference responses of zebrafish to 10 −3 , 10 −4 and 10 −5 M alanine (Ala) were concentration‐ dependent. Behavioural responses to copper (Cu) and Cu + Ala mixtures were also assessed. Zebrafish avoided 100 and 10 μg Cu l −1 , but not 1 μg l −1 . Mixtures of 10 −3 m Ala+ 100 μg Cu l −1 and 10 4 M Ala + 10 μg Cu 1 −1 were avoided as intensely as was Cu alone. Responses to 10 −3 M Ala + 10 or 1 μg Cu l −1 and 10 4 M Ala +1 μg Cu l −1 did not differ statistically from controls (no detectable preference or avoidance). These results demonstrate, firstly, that a concentration of a pollutant avoided by itself (10 μg Cu l −1 ) may not be avoided when encountered with an attractant chemical stimulus (Ala) and may suppress the preference for an attractant stimulus, and secondly, that a concentration of a pollutant not avoided by itself and not considered deleterious (1 μg Cu l −1 ) suppresses attraction to Ala (an important constituent of prey odours for many fishes).
An abundant nucleolar phosphoprotein is associated with ribosomal DNA in Tetrahymena macronuclei. (1997)

An abundant 52-kDa phosphoprotein was identified and characterized from macronuclei of the ciliated protozoan Tetrahymena thermophila. Immunoblot analyses combined with light and electron microscopic immunocytochemistry demonstrate that this polypeptide, termed Nopp52, is enriched in the nucleoli of transcriptionally active macronuclei and missing altogether from transcriptionally inert micronuclei. The cDNA sequence encoding Nopp52 predicts a polypeptide whose amino-terminal half consists of multiple acidic/serine-rich regions alternating with basic/proline-rich regions. Multiple serines located in these acidic stretches lie within casein kinase II consensus motifs, and Nopp52 is an excellent substrate for casein kinase II in vitro. The carboxyl-terminal half of Nopp52 contains two RNA recognition motifs and an extreme carboxyl-terminal domain rich in glycine, arginine, and phenylalanine, motifs common in many RNA processing proteins. A similar combination and order of motifs is found in vertebrate nucleolin and yeast NSR1, suggesting that Nopp52 is a member of a family of related nucleolar proteins. NSR1 and nucleolin have been implicated in transcriptional regulation of rDNA and rRNA processing. Consistent with a role in ribosomal gene metabolism, rDNA and Nopp52 colocalize in situ, as well as by cross-linking and immunoprecipitation experiments, demonstrating an association between Nopp52 and rDNA in vivo.
Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and epithelial cells in culture (1998)

Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non-permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell-cell interactions, possibly involving signalling via reactive oxygen species.
A Location Prediction Algorithm for Directional Communication (2008)

Directional antennas concentrate the transmitted power into a specified direction and form a directional beam pointing to a receiver. Directional communication can bring benefits in terms of power consumption, spatial reuse and security. Using direction antennas implies the transmitters must know the directions of receivers. In this paper, we propose an efficient algorithm to predict the future location of a mobile node in Ad hoc networks where nodes use directional antennas to communicate with each other. Our algorithm is based on the mobility characteristic we extracted from a real military mobile Ad hoc networks trace, and the algorithm efficiency is about 95.3%. Our algorithm can be employed by directional antennas with adaptive beamwidths or fixed beamwidth.
Acquired Immune Deficiency Syndrome in the United States: The First 1,000 Cases (1983)

Between June 1981 and February 1983, the Centers for Disease Control (Atlanta) received reports of 1,000 patients living in the United States who met a surveillance definition for the acquired immune deficiency syndrome (AIDS). Seventy-three percent of these patients were diagnosed after January 1, 1982. The 1,000 patients included 284 with Kaposi's sarcoma (KS), 497 with Pneumocystis carinii pneumonia (PCP), 83 with KS and PCP, and 136 with opportunistic infections other than PCP. The overall mortality has been 39.2%. Cases have been reported from 32 states and the District of Columbia; New York, California, New Jersey, and Florida account for 82.7% of the reports. All but 61 of the patients could be classified into one or more of the following groups: homosexual or bisexual men, intravenous drug abusers, Haitian natives, or patients with hemophilia. Epidemiologic trends in AIDS cases are consistent with the gradual extension of an infectious agent into new populations.
Synthesis and reactions of some novel imidazobenzoxazines and related systems (1982)

A novel synthesis of the new 4H-imidazo[2,1-c][1,4]benzoxazine system (2) has been carried out under two sets of conditions, leading to products with differing functionalities. The effect of substituents on the cyclization step has been investigated and the reactions of the functional groups of the new ring system have been studied. Derivatives of the related 4,5-dihydroimidazo[2,1 -d][1,5]benzoxazepine (3) have also been prepared.
Ultrafast imaging using gradient echoes. (1991)

Ultrafast magnetic resonance (MR) imaging techniques can reduce scan times to less than 1 s. The rapid acquisition minimizes motion artifacts that have plagued MR studies of the heart and abdomen, and facilitates dynamic studies to observe physiological function. We first discuss fast gradient-echo methods, including various spoiled and steady-state gradient-echo techniques. Ultrafast methods are then considered, with the focus on turbo-fast low-angle shot (FLASH) (also known as snapshot or subsecond FLASH) imaging. Although turbo-FLASH is a subset of gradient echo methods, there are several distinguishing features. For instance, with T1- or T2-weighted turbo-FLASH, the magnetization never reaches a steady state, so that the phase encode order becomes an important imaging parameter. Furthermore, image contrast is obtained by adjusting the magnetization preparation module, which is independent of the data acquisition module that follows. The signal behavior and strategies for contrast optimization are discussed. Potential clinical applications, including perfusion imaging, cardiac cine, breath-hold abdominal imaging, angiography, diffusion imaging, and three-dimensional studies, are explored.
Linkage and Association to Genetic Markers (2015)

Genetic markers that are sufficiently polymorphic (as measured by their heterozygosities) can be used in linkage and association analyses to detect Mendelian segregation underlying disease phenotypes. Each type of analysis can either be based on a specific genetic model or not make any assumptions about the mode of inheritance of the disease. Principles underlying these methods are reviewed, and the assumptions underlying them stressed. Association analyses are more powerful, provided there is linkage disequilibrium between the marker and disease loci; however, only linkage analyses have power in the absence of such disequilibrium. For this reason, models that allow for both kinds of tests are preferred, and such models must adequately approximate the complexity of the disease being studied.
Curved-channel microchannel array plates (1981)

Microchannel array plates (MCPs) in which the channels are curved to inhibit ion-feedback, in an identical manner to that employed in a conventional channel electron multiplier, have been fabricated and their performance characteristics have been evaluated. These devices represent a major advance in MCP technology since a single curved-channel MCP can be operated stably at high gain in the pulse-counting mode. Gains of greater than 106 electrons pulse−1 have been obtained at applied potentials of the order of 2000 V with maximum count-rate capabilities exceeding 105 counts mm−2s−1 and lifetimes exceeding 2.5×1011 counts mm−2.
Structures of reaction products from 1-hydrazinophthalazine and some carbonyls. (1976)

Reaction of 1-hydrazinophthalazine (1) with mesityl oxide gave five crystalline compounds, including hydrazones and their cyclization products. Isomerization of the hydrazones which were elucidated to be two geometrical isomers was discussed briefly. Structures of several products obtained by reactions of 1 with acetylacetone, tiglic aldehyde were also discussed on the basis of their spectral properties.
A Compact Polarization-Independent Wavelength Duplexer Using a Polarization-Diversity SOI Photonic Wire Circuit (2007)

We present a wavelength duplexer in silicon-on-insulator photonic wires. We made a polarization-diversity circuit using fiber grating couplers with polarization splitter and a bidirectional AWG, resulting in a polarization dependent loss of only 0.66dB.