gebhart/patrepeval huggingface dataset:
<i>TWIN SISTER OF FT</i>, <i>GIGANTEA</i>, and <i>CONSTANS</i> Have a Positive But Indirect Effect on Blue Light-Induced Stomatal Opening in Arabidopsis   (2013)

FLOWERING LOCUS T (FT) is the major regulatory component controlling photoperiodic floral transition. It is expressed in guard cells and affects blue light-induced stomatal opening induced by the blue-light receptor phototropins phot1 and phot2. Roles for other flowering regulators in stomatal opening have yet to be determined. We show in Arabidopsis (Arabidopsis thaliana) that TWIN SISTER OF FT (TSF), CONSTANS (CO), and GIGANTEA (GI) provide a positive effect on stomatal opening. TSF, which is the closest homolog of FT, was transcribed in guard cells, and light-induced stomatal opening was repressed in tsf-1, a T-DNA insertion mutant of TSF. Overexpression of TSF in a phot1 phot2 mutant background gave a constitutive open-stomata phenotype. Then, we examined whether CO and GI, which are upstream regulators of FT and TSF in photoperiodic flowering, are involved in stomatal opening. Similar to TSF, light-induced stomatal opening was suppressed in the GI and CO mutants gi-1 and co-1. A constitutive open-stomata phenotype was observed in GI and CO overexpressors with accompanying changes in the transcription of both FT and TSF. In photoperiodic flowering, photoperiod is sensed by photoreceptors such as the cryptochromes cry1 and cry2. We examined stomatal phenotypes in a cry1 cry2 mutant and in CRY2 overexpressors. Light-induced stomatal opening was suppressed in cry1 cry2, and the transcription of FT and TSF was down-regulated. In contrast, the stomata in CRY2 overexpressors opened even in the dark, and FT and TSF transcription was up-regulated. We conclude that the photoperiodic flowering components TSF, GI, and CO positively affect stomatal opening.
Dynamic Isoelectric Focusing for Proteomics (2006)

Dynamic isoelectric focusing is a new technique that is related to capillary isoelectric focusing but uses additional high-voltage power supplies to provide control over the shape of the electric field within the capillary. Manipulation of the electric field changes the pH gradient, enabling both the location and width of the focused protein bands to be controlled. The proteins can be migrated to a designated sampling point while remaining focused, where they can be collected for further analysis. This ability to collect and isolate the protein bands while maintaining a high peak capacity demonstrates that dynamic isoelectric focusing has great potential as a first dimension in a multidimensional separation system. Dynamic isoelectric focusing can achieve a peak capacity of over 1000, as shown by both mass spectrometry analysis and direct imaging.
Computational Study of Protein-Ligand Interaction with Application in Drug Discovery (2007)

Stony Brook University Libraries.\nSBU Graduate School in Chemistry.\nLawrence Martin (Dean of Graduate School), Jin Wang, Ph. D., Advisor\nDepartment of Chemistry, Stony Brook University, George Stell, Ph. D., Chairperson\nDepartment of Chemistry, Stony Brook University, Dale G. Drueckhammer, Ph. D., Third Member\nDepartment of Chemistry, Stony Brook University.
Input DM EMI filter design of interleaved CRM Boost PFC converter with coupled inductor (2011)

The interleaved Boost power factor correction (PFC) converter operating in critical current mode (CRM) features high input power factor, high efficiency and small magnetic components. If the inductors are coupled, the turns number can be reduced, but the input current ripple is increased. This paper discusses the two-phase interleaved CRM Boost converter with an inversely coupled inductor. The coupling effect on the input differential mode (DM) electromagnetic interface (EMI) filter is analyzed and the relations between input current ripple and DM noise are presented. The worst case of DM noise can be obtained by analyzing the input current ripple and experimental results verify the conclusions.
Dynamic management of TurboMode in modern multi-core chips (2014)

Dynamic overclocking of CPUs, or TurboMode, is a feature recently introduced on all x86 multi-core chips. It leverages thermal and power headroom from idle execution resources to overclock active cores to increase performance. TurboMode can accelerate CPU-bound applications at the cost of additional power consumption. Nevertheless, naive use of TurboMode can significantly increase power consumption without increasing performance. Thus far, there is no strategy for managing TurboMode to optimize its use across all workloads and efficiency metrics. This paper analyzes the impact of TurboMode on a wide range of efficiency metrics (performance, power, cost, and combined metrics such as QPS/W and ED2) for representative server workloads on various hardware configurations. We determine that TurboMode is generally beneficial for performance (up to +24%), cost efficiency (QPS/$ up to +8%), energy-delay product (ED, up to +47%), and energy-delay-squared product (ED2, up to +68%). However, TurboMode is inefficient for workloads that exhibit interference for shared resources. We use this information to build and validate a model that predicts the optimal TurboMode setting for each efficiency metric. We then implement autoturbo, a background daemon that dynamically manages TurboMode in real time without any hardware changes. We demonstrate that autoturbo improves QPS/$, ED, and ED2 by 8%, 47%, and 68% respectively over not using TurboMode. At the same time, autoturbo virtually eliminates all the large drops in those same metrics (-12%, -25%, -25% for QPS/$, ED, and ED2) that occur when TurboMode is used naively (always on).
Protecting private data in public (2006)

Current technologies allow users to access information in virtually any public setting. This creates situations where sensitive information, both organizational and personal in nature, can be seen and captured by nearby people and technology. Therefore, methods are necessary to ensure the privacy and security of information displayed in public spaces. The authors have developed Web browser privacy blinders, which hide sensitive information from view while leaving other information unobscured. Results of two pilot studies supported the viability and potential usefulness of the privacy blinder concept, and have set the stage for continued development of the technique through large-scale controlled studies and field tests.
PITUITARY AND ADRENAL RESPONSES TO THE ANTI-PROGESTERONE AND ANTI-GLUCOCORTICOID STEROID RU 486 IN PRIMATES1 (1983)

RU 486 is a synthetic steroid with anti-progesterone and anti-glucocorticoid properties. While studying its acute effects on pituitary hormone secretion in cynomolgus monkeys, we found that RU 486 inhibited PRL secretion induced by an estrogen-progesterone synergy (P less than 0.025). By contrast, plasma levels of ACTH, arginine vasopressin and cortisol increased following RU 486 administration (P less than 0.05). Plasma FSH, LH, GH and TSH were unaffected by RU 486 treatment. Our findings suggest potential diagnostic and therapeutic applications of RU 486.
Adeno-Associated Virus Vector-Mediated Minidystrophin Gene Therapy Improves Dystrophic Muscle Contractile Function in <i>mdx</i> Mice (2002)

Duchenne muscular dystrophy (DMD) is the most common disabling and lethal genetic muscle disorder, afflicting 1 of every 3500 males. Patients with DMD experience progressive muscle degeneration and weakness and succumb to respiratory or cardiac failure by their early twenties. No treatment is currently available for DMD. Mutations in the dystrophin gene result in lack of a functional dystrophin protein in striated muscle, which induces instability in the muscle cell membrane leading to persistent muscle injury after contraction. We have previously created novel minidystrophin genes and demonstrated that adeno-associated virus (AAV)-mediated intramuscular delivery of the minigenes effectively ameliorated mdx dystrophic histopathology and led to normal cell membrane integrity for more than 1 year. In this paper, we investigated whether AAV-minidystrophin could also improve mdx muscle contractile function. Two-month-old adult male mdx mice, with established muscular dystrophy, were given a single-dose injection of an AAV-minidystrophin vector in the tibialis anterior (TA) muscle of one leg, with the untreated contralateral leg used as a control. The treated TA muscle showed both (1) a significant increase in isometric force generation and (2) a significant increase in resistance to lengthening activation-induced muscle force decrements. We conclude that AAV-minidystrophin gene treatment is effective in improving mdx muscle contractile function.
Skeletal muscle phenotypes initiated by ectopic MyoD in transgenic mouse heart (1992)

Forced expression of the myogenic regulatory gene MyoD in many types of cultured cells initiates their conversion into skeletal muscle. It is not known, however, if MyoD expression serves to activate all or part of the skeletal muscle program in vivo during animal development, nor is it known how limiting the influences of cellular environment may be on the regulatory effects of MyoD. To begin to address these issues, we have produced transgenic mice which express MyoD in developing heart, where neither MyoD nor its three close relatives--myogenin, Myf-5, and MRF4/herculin/Myf-6--are normally expressed. The resulting gross phenotype in offspring from multiple, independent transgenic founders includes abnormal heart morphology and ultimately leads to death. At the molecular level, affected hearts exhibit activation of skeletal muscle-specific regulatory as well as structural genes. We conclude that MyoD is able to initiate the program that leads to skeletal muscle differentiation during mouse development, even in the presence of the ongoing cardiac differentiation program. Thus, targeted misexpression of this tissue-specific regulator during mammalian embryogenesis can activate, either directly or indirectly, a diverse set of genes normally restricted to a different cell lineage and a different cellular environment.
Regression Modeling (2009)

Regression Modeling: Methods, Theory, and Computation with SAS provides an introduction to a diverse assortment of regression techniques using SAS to solve a wide variety of regression problems. The author fully documents the SAS programs and thoroughly explains the output produced by the programs.The text presents the popular ordinary least square
Electronic and Vibrational Structure of Complexes of Tetracyanoquinodimethane with Cadmium Chalcogenide Quantum Dots (2014)

This paper describes the use of visible, near-infrared, and mid-infrared steady-state optical spectroscopy to study the geometries in which tetracyanoquinodimethane (TCNQ) adsorbs to the surfaces of highly cadmium enriched and near-stoichiometric CdSe quantum dots (QDs) in the formation of QD-TCNQ charge transfer (CT) complexes. Several TCNQ molecules are spontaneously reduced by chalcogenides on the surface of each CdSe QD. The degree of CT depends on the geometry with which the TCNQ adsorbs and the degree of distortion of TCNQ’s geometry upon adsorption. Comparison of the electronic and vibrational spectra of CdSe QD-TCNQ complexes with those of CT complexes of TCNQ with molecular reductants (including molecular chalcogenides) and computer simulations of the geometries and vibrational spectra of the TCNQ-chalcogenide CT complexes show that (i) the Cd-enriched CdSe QDs reduce a factor of 7.4 more TCNQ molecules per QD than nearly stoichiometric CdSe QDs because surface selenides are more accessible in the Cd-enriched QDs than in the near-stoichiometric QDs and (ii) TCNQ interacts with surface selenides through several adsorption modes that result in different amounts of charge transfer and different degrees of geometric distortion of TCNQ. This study provides a framework for determining the range of adsorption geometries of small molecules on QD surfaces, and for optimizing QD surfaces to adsorb molecules in configurations with maximal electronic coupling between the QD and the adsorbate.
A novel method to quantitate methylation of specific genomic regions. (1994)

A new solid-phase primer extension method has been developed for the quantitation of methylation differences and is described here. The method is less cumbersome than Southern blot analysis, expresses the results in a numerical format, can be adapted to a microtitration well format, and thus allows the analysis of a large series of samples. The model gene analyzed here is the calcitonin gene, but the method can be adapted to the analysis of methylation alterations in any area of the genome. The primer extension method clearly differentiated hypermethylated samples from normally methylated samples and a range for normal values could be determined. In quantitation experiments the method showed linearity in a range from 2% to 100% malignant blasts diluted with normal leukocytes.
Fieldbus application layer and real-time distributed systems (2002)

The author presents the application layer of Fieldbus as part of a real-time distributed system. The services of the layer must warrant the distributed process synchronization in real-time, and temporal and spatial data consistency. A model that fulfils these requirements is presented and a protocol with very low network and computational overhead is proposed. This protocol is intended to be used in low-cost Fieldbus for hard real-time applications. The proposed model is discussed in detail and compared with other application layer models intended for Fieldbus. The communication protocol that implements this model is also discussed in detail.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Steroids. CXIII.<sup>1</sup> 6-Methyl Estrogens (1959)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSteroids. CXIII.1 6-Methyl EstrogensESPERANZA VELARDE, J. IRIARTE, H. J. RINGOLD, and CARL DJERASSICite this: J. Org. Chem. 1959, 24, 3, 311–313Publication Date (Print):March 1, 1959Publication History Published online1 May 2002Published inissue 1 March 1959https://pubs.acs.org/doi/10.1021/jo01085a006https://doi.org/10.1021/jo01085a006research-articleACS PublicationsRequest reuse permissionsArticle Views71Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optical fibres with an Al <sub>2</sub> O <sub>3</sub> -doped silicate core composition (1983)

Optical fibres consisting of Al2O3-SiO2 and Al2O3-P2O5-SiO2 cores have been fabricated by MCVD with a minimum attenuation of 0.7 dB/km at 1.6 μm. Both single-mode and multimode fibres were produced, the latter with a numerical aperture as high as 0.29. The tendency for Al2O3-SiO2 glasses produced by flame hydrolysis processes to devitrify during consolidation is diminished by MCVD processing, thus allowing higher Al2O3 doping and versatility with regard to the incorporation of other ions.
Identification and preliminary characterization of an O6-methylguanine DNA repair methyltransferase in the yeast Saccharomyces cerevisiae. (1990)

Saccharomyces cerevisiae contains a DNA repair methyltransferase (MTase) that repairs O6-methylguanine. Methyl groups are irreversibly transferred from O6-methylguanine in DNA to a 25-kilodalton protein in S. cerevisiae cell extracts, and methyl transfer is accompanied by the formation of S-methylcysteine. The yeast MTase is expressed at approximately 150 molecules/cell in exponentially growing yeast cultures but is not detectable in stationary phase cells. Unlike mammalian and bacterial MTases, the yeast MTase is very temperature-sensitive, having a half-life of about 4 min at 37 degrees C, which may explain why others have failed to detect it. Like other DNA repair MTases, the S. cerevisiae MTase repairs O6-methylguanine more efficiently in double-stranded DNA than in single-stranded DNA. Synthesis of the yeast DNA MTase is apparently not inducible by sublethal exposures to alkylating agent, but rather MTase activity is depleted in cells exposed to low doses of alkylating agent. Judging from its molecular weight and substrate specificity, the yeast DNA MTase is more closely related to mammalian MTases than to Escherichia coli MTases.
GLUT4 and Transferrin Receptor Are Differentially Sorted Along the Endocytic Pathway in CHO Cells (1998)

The trafficking of GLUT4, a facilitative glucose transporter, is examined in transfected CHO cells. In previous work, we expressed GLUT4 in neuroendocrine cells and fibroblasts and found that it was targeted to a population of small vesicles slightly larger than synaptic vesicles (Herman, G.A, F. Bonzelius, A.M. Cieutat, and R.B. Kelly. 1994. Proc. Natl. Acad. Sci. USA. 91: 12750-12754.). In this study, we demonstrate that at 37 degrees C, GLUT4-containing small vesicles (GSVs) are detected after cell surface radiolabeling of GLUT4 whereas uptake of radioiodinated human transferrin does not show appreciable accumulation within these small vesicles. Immunofluorescence microscopy experiments show that at 37 degrees C, cell surface-labeled GLUT4 as well as transferrin is internalized into peripheral and perinuclear structures. At 15 degrees C, endocytosis of GLUT4 continues to occur at a slowed rate, but whereas fluorescently labeled GLUT4 is seen to accumulate within large peripheral endosomes, no perinuclear structures are labeled, and no radiolabeled GSVs are detectable. Shifting cells to 37 degrees C after accumulating labeled GLUT4 at 15 degrees C results in the reappearance of GLUT4 in perinuclear structures and GSV reformation. Cytosol acidification or treatment with hypertonic media containing sucrose prevents the exit of GLUT4 from peripheral endosomes as well as GSV formation, suggesting that coat proteins may be involved in the endocytic trafficking of GLUT4. In contrast, at 15 degrees C, transferrin continues to traffic to perinuclear structures and overall labels structures similar in distribution to those observed at 37 degrees C. Furthermore, treatment with hypertonic media has no apparent effect on transferrin trafficking from peripheral endosomes. Double-labeling experiments after the internalization of both transferrin and surface-labeled GLUT4 show that GLUT4 accumulates within peripheral compartments that exclude the transferrin receptor (TfR) at both 15 degrees and 37 degrees C. Thus, GLUT4 is sorted differently from the transferrin receptor as evidenced by the targeting of each protein to distinct early endosomal compartments and by the formation of GSVs. These results suggest that the sorting of GLUT4 from TfR may occur primarily at the level of the plasma membrane into distinct endosomes and that the organization of the endocytic system in CHO cells more closely resembles that of neuroendocrine cells than previously appreciated.
Phosphororganische Verbindungen, 52. Beitrag zum Mechanismus der Arylphosphoniumsalzbildung aus tertiären Phosphinen nach der „Kobaltsalz‐Methode” (1966)

Abstract Die bei der Kharasch‐Reaktion erzeugten Aryl‐Radikale werden von tertiären Phosphinen unter Aufbau einer P‐Arylbindung abgefangen. Die Ausbeuten an quartären Phosphoniumsalzen werden studiert in Abhängigkeit von der Menge an Kobaltsalz, von der Natur des Lösungsmittels, von der Lösungsmittelmenge, von der Natur des Arylhalogenids, vom Anion des Kobaltsalzes, von der Reihenfolge der Substanzzugabe und von der Menge an gelöstem Kobaltchlorid in Diäthyläther. — In aliphatisch und aliphatisch‐aromatisch substituierten tertiären Phosphinen werden die aliphatischen Liganden — mit hoher Wahrscheinlichkeit auf der Stufe der tertiären Phosphine — durch Aryl‐Radikale verdrängt. Die experimentellen Befunde für diesen Radikalprozess werden mitgeteilt; ein Mechanismus wird zur Diskussion gestellt.
Demonstration that <i>fbiC</i> Is Required by <i>Mycobacterium bovis</i> BCG for Coenzyme F <sub>420</sub> and FO Biosynthesis (2002)

Using the nitroimidazopyran-based antituberculosis drug PA-824 as a selective agent, transposon-generated Mycobacterium bovis strain BCG (M. bovis) mutants that could not make coenzyme F(420) were identified. Four independent mutants that could not make F(420) or the biosynthesis intermediate FO were examined more closely. These mutants contained transposons inserted in the M. bovis homologue of the Mycobacterium tuberculosis gene Rv1173, which we have named fbiC. Complementation of an M. bovis FbiC(-) mutant with fbiC restored the F(420) phenotype. These data demonstrate that fbiC is essential for F(420) production and that FbiC participates in a portion of the F(420) biosynthetic pathway between pyrimidinedione and FO. Homologues of fbiC were found in all 11 microorganisms that have been fully sequenced and that are known to make F(420). Four of these homologues (all from members of the aerobic actinomycetes) coded for proteins homologous over the entire length of the M. bovis FbiC, but in seven microorganisms two separate genes were found to code for proteins homologous with either the N-terminal or C-terminal portions of the M. bovis FbiC. Histidine-tagged FbiC overexpressed in Escherichia coli produced a fusion protein of the molecular mass predicted from the M. bovis BCG sequence (approximately 95,000 Da), as well as three other histidine-tagged proteins of significantly smaller size, which are thought to be proteolysis products of the FbiC fusion protein.
Observations on the Etiology of Trigeminal Neuralgia, Hemifacial Spasm, Acoustic Nerve Dysfunction and Glossopharyngeal Neuralgia. Definitive Microsurgical Treatment and Results in 117 Patients (1977)

Microsurgical observations have been made of the cranial nerve root entry or exit zones 117 patients operated upon for the treatment of hyperactive-hypoactive dysfunction syndromes (trigeminal neuralgia, hemifacial spasm, acoustic nerve dysfunction, and glossopharyngeal neuralgia). Cross-compression or distortion of the appropriate nerve root at its entry or exit zone was noted in all patients. This compression or distortion was usually caused by normal or arteriosclerotic, elongated arterial loops, it was usually relieved by decompressive microsurgical techniques. A small percentage of patients were found to have compression of the nerve root at the entry-exit zone by a tumor, a vein, or some other structural abnormality; they were relieved by tumor excision or other measures as described. Relief was gradual postoperatively if the treated nerve was not stroked or manipulated at operation but it was immediate if the nerve was manipulated. Preoperative evidence of decreased nerve function improved postoperatively.