gebhart/patrepeval huggingface dataset:
Physical and Functional Interaction of the Arabidopsis K<sup>+</sup> Channel AKT2 and Phosphatase AtPP2CA (2002)

The AKT2 K(+) channel is endowed with unique functional properties, being the only weak inward rectifier characterized to date in Arabidopsis. The gene is expressed widely, mainly in the phloem but also at lower levels in leaf epiderm, mesophyll, and guard cells. The AKT2 mRNA level is upregulated by abscisic acid. By screening a two-hybrid cDNA library, we isolated a protein phosphatase 2C (AtPP2CA) involved in abscisic acid signaling as a putative partner of AKT2. We further confirmed the interaction by in vitro binding studies. The expression of AtPP2CA (beta-glucuronidase reporter gene) displayed a pattern largely overlapping that of AKT2 and was upregulated by abscisic acid. Coexpression of AtPP2CA with AKT2 in COS cells and Xenopus laevis oocytes was found to induce both an inhibition of the AKT2 current and an increase of the channel inward rectification. Site-directed mutagenesis and pharmacological analysis revealed that this functional interaction involves AtPP2CA phosphatase activity. Regulation of AKT2 activity by AtPP2CA in planta could allow the control of K(+) transport and membrane polarization during stress situations.
A molecular understanding of alphavirus entry (2020)

Alphaviruses cause severe human illnesses including persistent arthritis and fatal encephalitis. As alphavirus entry into target cells is the first step in infection, intensive research efforts have focused on elucidating aspects of this pathway, including attachment, internalization, and fusion. Herein, we review recent developments in the molecular understanding of alphavirus entry both in vitro and in vivo and how these advances might enable the design of therapeutics targeting this critical step in the alphavirus life cycle.
Signal Detection Methods for Magnetostrictive Sensors (1997)

<div class="htmlview paragraph">Magnetostrictive sensors offer many attractive features. Their strain sensitivity is significantly higher than that of strain gages. They are simple, rugged and inexpensive. Signal conditioning requirements for the magnetostrictive sensors are determined by the selection of a signal detection method. This article experimentally investigates different signal detection methods for single branch magnetostrictive sensors with conventional excitation and detection coils. Monitoring excitation voltage while holding excitation current constant, or monitoring excitation current while holding excitation voltage constant produces much better output signal compared to the detection voltage. Furthermore, this study suggest that the detection coil is not really needed, leading to a novel single branch magnetostrictive sensor without a detection coil.</div>
Identification and Characterization of <i>alcR</i> , a Gene Encoding an AraC-Like Regulator of Alcaligin Siderophore Biosynthesis and Transport in <i>Bordetella pertussis</i> and <i>Bordetella bronchiseptica</i> (1998)

A Bordetella bronchiseptica iron transport mutant was isolated following an enrichment procedure based on streptonigrin resistance. The mutant displayed a growth defect on iron-restricted medium containing ferric alcaligin as the sole iron source. In addition to the apparent inability to acquire iron from the siderophore, the mutant failed to produce alcaligin as well as two known iron-regulated proteins, one of which is the AlcC alcaligin biosynthesis protein. A 1.6-kb KpnI-PstI Bordetella pertussis DNA fragment mapping downstream of the alcaligin biosynthesis genes alcABC restored both siderophore biosynthesis and expression of the iron-regulated proteins to the mutant. Nucleotide sequencing of this complementing 1.6-kb region identified an open reading frame predicted to encode a protein with strong similarity to members of the AraC family of transcriptional regulators, for which we propose the gene designation alcR. Primer extension analysis localized an iron-regulated transcription initiation site upstream of the alcR open reading frame and adjacent to sequences homologous to the consensus Fur repressor binding site. The AlcR protein was produced by using an Escherichia coli expression system and visualized in electrophoretic gels. In-frame alcR deletion mutants of B. pertussis and B. bronchiseptica were constructed, and the defined mutants exhibited the alcR mutant phenotype, characterized by the inability to produce and transport alcaligin and express the two iron-repressed proteins. The cloned alcR gene provided in trans restored these siderophore system activities to the mutants. Together, these results indicate that AlcR is involved in the regulation of Bordetella alcaligin biosynthesis and transport genes and is required for their full expression.
A Qualitative Discrepancy between Censored Data Rank Tests (1979)

Two popular censored data rank tests were used to compare cancer incidence rates between dogs receiving bone marrow transplantation and control dogs. The logrank test gave a significance level of 0.01. In contrast, Gehan's generalized Wilcoxon test gave a significance level of 0.76. The statistics are displayed in a manner that shows how such contrasting significance levels can arise. The Gehan statistic is shown to be subject to a serious criticism that does not apply to other Wilcoxon generalizations. Further insight into the data is obtained using a time-dependent logrank test and the proportional hazards regression model.
Acellular Pertussis Vaccine Protects against Exacerbation of Allergic Asthma Due to<i>Bordetella pertussis</i>in a Murine Model (2005)

The prevalence of asthma and allergic disease has increased in many countries, and there has been speculation that immunization promotes allergic sensitization. Bordetella pertussis infection exacerbates allergic asthmatic responses. We investigated whether acellular pertussis vaccine (Pa) enhanced or prevented B. pertussis-induced exacerbation of allergic asthma. Groups of mice were immunized with Pa, infected with B. pertussis, and/or sensitized to ovalbumin. Immunological, pathological, and physiological changes were measured to assess the impact of immunization on immune deviation and airway function. We demonstrate that immunization did not enhance ovalbumin-specific serum immunoglobulin E production. Histopathological examination revealed that immunization reduced the severity of airway pathology associated with sensitization in the context of infection and decreased bronchial hyperreactivity upon methacholine exposure of infected and sensitized mice. These data demonstrate unequivocally the benefit of Pa immunization to health and justify selection of Pa in mass vaccination protocols. In the absence of infection, the Pa used in this study enhanced the interleukin-10 (IL-10) and IL-13 responses and influenced airway hyperresponsiveness to sensitizing antigen; however, these data do not suggest that Pa contributes to childhood asthma overall. On the contrary, wild-type virulent B. pertussis is still circulating in most countries, and our data suggest that the major influence of Pa is to protect against the powerful exacerbation of asthma-like pathology induced by B. pertussis.
Application-Aware Traffic Redirection: A Mobile Edge Computing Implementation Toward Future 5G Networks (2017)

With the development of network technology, there are billions of devices accessing resources and services on the cloud through mobile telecommunication network. A great number of connections and data packets must be handled by the mobile network. It not only consumes the limited spectrum resources and network bandwidth, but also reduces the service quality of applications. To alleviate the problem, the concept of Mobile Edge Computing (MEC) has been proposed by European Telecommunications Standard Institute (ETSI) in 2014. MEC suggests to provide IT and cloud computing capabilities at the network edge to offer low-latency and high-bandwidth service. The architecture and the benefits of MEC have been discussed in many recent literature. But the implementation of underlying network is rarely discussed or evaluated in practice. In this paper, we present our prototype implementation of a MEC platform by developing an application-aware traffic redirection mechanism at edge network to reduce service latency and network bandwidth consumption. Our implementation is based on OAI, an open source project of 5G SoftRAN cellular system. To the best of our knowledge, it is also one of the few MEC solutions that have been built for 5G networks in practice.
Isoelectric focussing behavior of bovine plasma albumin, mercaptalbumin, and β-lactoglobulins A and B (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsoelectric focussing behavior of bovine plasma albumin, mercaptalbumin, and β-lactoglobulins A and BJoseph F. Foster and Lawrence J. KaplanCite this: Biochemistry 1971, 10, 4, 630–636Publication Date (Print):February 16, 1971Publication History Published online1 May 2002Published inissue 16 February 1971https://pubs.acs.org/doi/10.1021/bi00780a014https://doi.org/10.1021/bi00780a014research-articleACS PublicationsRequest reuse permissionsArticle Views199Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Overview of Oral Cancer (2012)

Cancer is the second most common cause of death in the Western world, after cardiovascular diseases (Johnson, 1991; 2001). Worldwide, an estimated cancer incidence of about 10 million was reported for the year 2009 (Jemal et al., 2010), and 1 out of every 3 persons is estimated to suffer from cancer by the age of 75 years (Johnson, 1991; 2001). It is also estimated that about 7.9 million people world-wide will die from cancer this year (Jemal et al., 2010), accounting for nearly 12% of deaths worldwide (Jemal et al., 2010). In the United States alone, an estimated 569,490 deaths from cancer are projected for the 2010 (Jemal et al., 2010). Recent published estimates of worldwide frequency of the 16 major cancers indicate that in developing countries with a high prevalence of infectious and nutritional diseases, cancer remains a major cause of death (Parkin, Laara and Muir, 1988). This may account partly for the current statistics whereby more than half the global incidence of cancer is from the so-called developing countries, since an estimated 70-80% of the global population resides in these areas (Parkin et al., 1998). The estimated annual incidence of cancer ranges from 48 to 225 per 100,000 in developing countries (Parkin et al., 1998).
Fibronectin‐binding proteins of <i>Staphylococcus aureus</i> mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcγRIIa receptor (2005)

Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we report that the fibrinogen/fibronectin-binding proteins FnBPA and FnBPB are major platelet-activating factors on the surface of S. aureus from the exponential phase of growth. Truncated derivatives of FnBPA, presenting either the fibrinogen-binding A domain or the fibronectin-binding BCD region, each promoted platelet activation when expressed on the surface of S. aureus or Lactococcus lactis, indicating two distinct mechanisms of activation. FnBPA-promoted platelet activation is mediated by fibrinogen and fibronectin bridges between the A domain and the BCD domains, respectively, to the low affinity form of the integrin GPIIb/IIIa on resting platelets. Antibodies recognizing the FnBPA A domain or the complex between the FnBPA BCD domains and fibronectin were essential for activation promoted by bacteria expressing the A domain or the BCD domain respectively. Activation was inhibited by a monoclonal antibody (IV-3) specific for the FcgammaRIIa IgG receptor on platelets. We propose that the activation of quiescent platelets by bacteria expressing FnBPs involves the formation of a bridge between the bacterial cell and the platelet surface by (i) fibronectin and fibrinogen interacting with the low affinity form of GPIIb/IIIa and (ii) by antibodies specific to FnBPs that engage the platelet Fc receptor FcgammaRIIa. Platelet activation by S. aureus clinical IE isolates from both the exponential and stationary phases of growth was completely inhibited by monoclonal antibody IV-3 suggesting that the IgG-FcgammaRIIa interaction is of fundamental importance for platelet activation mediated by this organism. This suggests new avenues for development of therapeutics against vascular infections.
A mathematical modeling approach to quantify the role of phlebotomy losses and need for transfusions in neonatal anemia (2012)

The present modeling results indicate that while phlebotomy reduction can significantly decrease the number of RBCTx administered to preterm infants, total elimination of all RBCTx will likely require other approaches, for example, stimulation of erythropoiesis with erythropoiesis-stimulating agents.
Simple CMOS current-mode exponential function generator circuit (2008)

A low-power and low-voltage simple CMOS exponential function generator circuit is proposed. It is based on simple current mirrors able to operate at low supply voltage. The design of the circuit is based on Taylor series expression for realizing the exponential characteristic and of MOS transistor operating in the saturation region. Simulation using the PSPICE program with 0.5 mum CMOS model of MIETEC. The simulation results show a 16 dB linear output current range with the linearity error less than 1 dB at 1.5 V supply voltage.
Interlaboratory Validation of the Vidas SET2 Kit for Detection of Staphylococcal Enterotoxins in Milk Products (2007)

An earlier intralaboratory validation study based on the EN ISO 16140 Standard conducted by the Community Reference Laboratory for coagulase-positive staphylococci including Staphylocococcus aureus showed that, after an extraction step using dialysis concentration, the Vidas SET2 detection kit could be used to screen staphylococcal enterotoxins in milk and milk products. In order to fully validate Vidas SET2, an interlaboratory study was organized. Six freeze-dried samples and 3 ready-to-use concentrated extracts were analyzed by 21 laboratories according to the method, including a detection with Vidas SET2. Results did not show false-positive or -negative results. Accordance and concordance parameters were equal to 100%, corresponding to a concordance odds ratio of 1. This interlaboratory study confirmed the satisfactory outcome of the preliminary tests and of the intralaboratory study performed previously. The Vidas SET2 detection kit can be used as a method for the detection of staphylococcal enterotoxins in milk and milk products as well as the Transia Plate SET detection kit in the European screening method for official control purposes, after an extraction step followed by dialysis concentration.
Is Oil Palm the Next Emerging Threat to the Amazon? (2009)

The Amazon Basin appears poised to experience rapid expansion of oil palm agriculture. Nearly half of Amazonia is suitable for oil palm cultivation, and Malaysian corporations are now moving into the region to establish new plantations while the Brazilian government is considering a law that would count oil palm as “forest” towards a landowner's forest reserve requirement. Strong economic incentives for a major Amazonian oil palm industry are likely, given growing global demands for edible oils, oil-based products, and biofuel feedstocks. We have two main concerns. First, oil palm plantations are ecologically depauperate, supporting little forest-dependent wildlife. Second, we disbelieve political and corporate statements suggesting that oil palm plantations will be concentrated on previously deforested lands in Amazonia. In reality, oil palm producers strongly favor clearing primary forest for plantations because they can reap immediate profits from timber production. These profits subsidize the costs of plantation establishment and maintenance for the initial 3-5 years until the oil palm plantations become profitable. Hence, oil palm agriculture could soon emerge as a major new threat to the Amazonian environment.
Muscle Quality, Aerobic Fitness and Fat Mass Predict Lower-Extremity Physical Function in Community-Dwelling Older Adults (2007)

Muscle quality is the most important predictor, and aerobic fitness and fat mass are secondary predictors of LEPF in community-dwelling older adults. These findings support the importance of exercise, especially strength training, for optimal body composition, and maintenance of strength and physical function in older adults.
The intracellular distribution patterns of myosin and actin are different among human neutrophils and monocytes during locomotion. (1998)

Findings indicate that differing patterns of arrangement of myosin and actin in the lamellipodia and cytoplasm of neutrophils and monocytes may contribute to their movement, in vitro.
Vision Based Modeling of Plants Phenotyping in Vertical Farming under Artificial Lighting (2019)

In this paper, we present a novel method for vision based plants phenotyping in indoor vertical farming under artificial lighting. The method combines 3D plants modeling and deep segmentation of the higher leaves, during a period of 25-30 days, related to their growth. The novelty of our approach is in providing 3D reconstruction, leaf segmentation, geometric surface modeling, and deep network estimation for weight prediction to effectively measure plant growth, under three relevant phenotype features: height, weight and leaf area. Together with the vision based measurements, to verify the soundness of our proposed method, we also harvested the plants at specific time periods to take manual measurements, collecting a great amount of data. In particular, we manually collected 2592 data points related to the plant phenotype and 1728 images of the plants. This allowed us to show with a good number of experiments that the vision based methods ensure a quite accurate prediction of the considered features, providing a way to predict plant behavior, under specific conditions, without any need to resort to human measurements.
Dendritic and Fractal Patterns in Electrolytic Metal Deposits (1986)

Pattern formation in the electrodeposition of Zn from a thin layer of ZnS${\mathrm{O}}_{4}$ solution was studied as a function of electrolyte concentration and applied voltage. We found several qualitatively different growth forms in this system. Most strikingly, a transition from dendritic crystals (where crystalline anisotropy dominates) to disordered ramified patterns is found when the electrolyte concentration is reduced. The disordered patterns may be described as fractal below a concentration-dependent cutoff length.
A study of optical parameters of amorphous Se90InxGe10- x thin films before and after heat treatment (2007)

Amorphous thin film of Se 90 In x Ge 10-x (x = 2 and 4) were thermally evaporated onto chemically cleaned glass substrates. Some optical parameters of these films were studied before and after thermal annealing above glass transition temperature of the respective alloys. The optical transmission spectra were measured in thin films of the above glasses in the wavelength range 400-2000 nm using spectrophotometer. The straight forward analysis proposed by Swanepoel has been successfully employed to determine the optical constants. It is observed from the measurements that the values of refractive index (n) and real dielectric constant (e') decrease with increase in wavelength while extinction coefficient (k) and imaginary dielectric constant (e) increase with increase in wavelength. The absorption coefficient (a) measured at strong absorption region also increases with photon energy (hv). Optical band gap (Eg) decreases with In concentration and also after thermal annealing of the films above glass transition temperature. This decrease in band gap could be accounted for by the generation of excess electronic delocalized states on incorporation of Indium as well as on thermal annealing in binary Se-Ge system.
Robust Algorithm for Heart Rate (HR) Detection and Heart Rate Variability (HRV) Estimation (2010)

We present algorithm for Heart Rate detection based on Short-Term Autocorrelation Center Clipping method. This algorithm is dedicated for biological signal detection, electrocardiogram, in noisy environment with lot of artifacts. Using this algorithm is also possible detect the R pointers in the PQRST complex of the ECG signal. In this paper the new implementation of the heart rate variability estimation is also presented. HRV module is based on parametric and non-parametric methods of the power spectral density computation.
The primary structure of bovine monoamine oxidase type A. Comparison with peptide sequences of bovine monoamine oxidase type B and other flavoenzymes (1989)

We have isolated cDNA clones believed to encompass the full-length coding sequences for a subunit of bovine monoamine oxidase type A (MAO-A). The clones code for an apoprotein of 527 amino acid residues corresponding to a molecular mass of 59,806 Da. The inferred protein sequences show an overall similarity of 68% with partial amino acid sequences of bovine type B MAO (about 41% of the total sequence), as well as a greater similarity (greater than 90%) with some regions including that for the published sequence of the flavin-binding region. Sequence comparisons indicate that these two forms of MAO are encoded by distinct genes. Comparison of this sequence with other flavoenzymes showed similarity with regions associated with non-covalent flavin-binding sites. Analysis of mRNAs coding for MAO enzymes showed a heterogeneity of transcripts consistent with several different forms of monoamine oxidase.
Echocardiographic Assessment of Ductus Arteriosus Shunt in Premature Infants (1974)

Echocardiograms were performed on premature infants with a patent ductus arteriosus (PDA) in order to assess the magnitude of the left-to-right shunt. Ten of 14 infants with a hemodynamically significant PDA required ligation because of intractable cardiac failure. Twenty infants served as controls. These consisted of seven infants without heart disease, ten infants who were examined echocardiographically after surgical ligation of a PDA, and three infants who were considered to have closed the PDA spontaneously prior to echocardiographic examination. We measured the left atrial (LA) and aortic root (Ao) diameters and an LA/Ao ratio was used to assess left atrial size. The LA/Ao ratio was 1.28 ± 0.23 ( sd ) in the group with PDA and 0.86 ± 0.10 in the control group. Infants who required surgical ligation all had an LA/Ao ratio above 1.15 (mean 1.38 ± 0.19). After ligation of the PDA the ratio returned to normal levels (0.87 ± 0.12). Sequential studies performed on seven infants demonstrated the usefulness of the LA/Ao ratio in following the changes in left-to-right ductus shunting and left ventricular failure as well as the effects of medical and surgical therapy. The LA/Ao ratio is helpful in differentiating infants with respiratory distress due to ductus shunting and left ventricular failure from intrinsic pulmonary disease.