gebhart/patrepeval huggingface dataset:
The gene for human leukemia inhibitory factor (LIF) maps to 22q12. (1989)

The gene for human leukemia inhibitory factor (LIF) has been mapped by Southern analysis of a series of mouse/human somatic cell hybrids and by in situ hybridization to the chromosomes of two normal males and some individuals with chromosomal rearrangements. The gene maps to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2. From the grain distribution over high resolution chromosome preparations, the most likely location is 22q12.1----q12.2. Southern analysis of DNA from one Ewing sarcoma with t[11;22][q24;q12] showed that the breakpoint on chromosome 22 is more than 15 kb 5' or 8 kb 3' from the LIF gene. The location of the LIF gene indicates that translocations of this gene are unlikely to play a role in myeloid leukemia and myeloproliferative disorders.
Partial Purification of An Oxygen Scavenging Cell Membrane Fraction for use in Anaerobic Biochemical Reactions (1987)

Anaerobic conditions are necessary to prevent the autoxidation of tetrahydrobiopterin. An enzymatic method for achieving such an anaerobic condition is shown to be obtained by a lactate oxidase activity in the membrane of Escherichia coli when the enzyme concentration exceeds 0.05 unit/ml. A procedure is described for partial purification of the membrane fragment that involves salt precipitation and gel sieve chromatography on Bio-Gel A-50m. The rate of removal of oxygen is used to define the enzyme activity, to determine stability during storage, and to define conditions for stabilization of tetrahydrobiopterin. A second assay procedure that measures the rate of reduction of resazurin is described and is useful when the enzyme concentration is low.
Autoantibodies in IDDM Primarily Recognize the 65,000-<i>M</i>r Rather Than 67,000-<i>M</i>r Isoform of Glutamic Acid Decarboxylase (1993)

Glutamic acid decarboxylase autoantibodies may aid in rapid screening strategies predicting IDDM before clinical onset. Rat islets contain GAD65 and GAD67 autoantibody targets, but human islets express only GAD65, now confirmed by direct immunoprecipitation from radiolabeled rat and human islets. Because human IDDM involves beta-cell-specific autoimmunity, we tested 190 new IDDM patients and 51 healthy control subjects for antibodies to recombinant human islet GAD65, rat islet GAD67, or human insulinoma/cerebellum GAD67, each expressed separately in hamster fibroblasts. By using immunoprecipitation, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and densitometric fluorogram scanning, 132 of 190 (70%) of new IDDM patients had GAD65 autoantibodies, whereas only 17 of 190 (9%) had antibodies to rat GAD67 (P < 0.001). Of healthy control subjects, 2 of 51 (3.9%) and 1 of 51 (1.9%) had antibodies to GAD65 and GAD67, respectively. All 17 GAD67 antibody-positive patients also had GAD65 antibodies; 14 of 17 with greater GAD65 than GAD67 index. Control studies showed comparable reactivity between recombinant rat and human GAD67 and between different subcellular preparations of recombinant GAD67 of either species. In conclusion, only GAD65 is expressed in human islets, the autoantibody response is primarily to this isoform, and GAD67 antibodies add little to IDDM detection.
Stabilization of three time-varying linear systems using ZG method with pseudo division-by-zero phenomena displayed (2017)

In this paper, we research the stabilization of three time-varying linear (TVL) systems with pseudo division-by-zero (PDBZ) phenomena displayed. Thereinto, Zhang-gradient (also known as zeroing-gradient, ZG) method consisting of Zhang neurodynamics (ZN) and gradient neurodynamics (GN) is applied to designing three ZG stabilization controllers. Through correlative numerical experiments, we more intuitively see that the ZG method is capable of realizing the stabilization control of three TVL systems. In addition, the ZG method is also able to solve the PDBZ (pseudo division-by-zero) problems. Moreover, we discuss the effects of design parameters as well as random initial values on three TVL systems, respectively.
Covert channel vulnerabilities in anonymity systems (2021)

The spread of wide-scale Internet surveillance has spurred interest in anonymity systems that protect users’ privacy by restricting unauthorised access to their identity. This requirement can be considered as a flow control policy in the well established field of multilevel secure systems. I apply previous research on covert channels (unintended means to communicate in violation of a security policy) to analyse several anonymity systems in an innovative way. One application for anonymity systems is to prevent collusion in competitions. I show how covert channels may be exploited to violate these protections and construct defences against such attacks, drawing from previous covert channel research and collusion-resistant voting systems. In the military context, for which multilevel secure systems were designed, covert channels are increasingly eliminated by physical separation of interconnected single-role computers. Prior work on the remaining network covert channels has been solely based on protocol specifications. I examine some protocol implementations and show how the use of several covert channels can be detected and how channels can be modified to resist detection. I show how side channels (unintended information leakage) in anonymity networks may reveal the behaviour of users. While drawing on previous research on traffic analysis and covert channels, I avoid the traditional assumption of an omnipotent adversary. Rather, these attacks are feasible for an attacker with limited access to the network. The effectiveness of these techniques is demonstrated by experiments on a deployed anonymity network, Tor. Finally, I introduce novel covert and side channels which exploit thermal effects. Changes in temperature can be remotely induced through CPU load and measured by their effects on crystal clock skew. Experiments show this to be an effective attack against Tor. This side channel may also be usable for geolocation and, as a covert channel, can cross supposedly infallible air-gap security boundaries. This thesis demonstrates how theoretical models and generic methodologies relating to covert channels may be applied to find practical solutions to problems in real-world anonymity systems. These findings confirm the existing hypothesis that covert channel analysis, vulnerabilities and defences developed for multilevel secure systems apply equally well to anonymity systems.
Methicillin-Resistant<i>Staphylococcus aureus</i>Carriage and Risk Factors for Skin Infections, Southwestern Alaska, USA (2010)

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are common in southwestern Alaska. Outbreak strains have been shown to carry the genes for Panton-Valentine leukocidin (PVL). To determine if carriage of PVL-positive CA-MRSA increased the risk for subsequent soft tissue infection, we conducted a retrospective cohort study by reviewing the medical records of 316 persons for 3.6 years after their participation in a MRSA nasal colonization survey. Demographic, nasal carriage, and antimicrobial drug use data were analyzed for association with skin infection risk. Skin infections were more likely to develop in MRSA carriers than in methicillin-susceptible S. aureus carriers or noncarriers of S. aureus during the first follow-up year, but not in subsequent years. Repeated skin infections were more common among MRSA carriers. In an area where PVL-containing MRSA is prevalent, skin infection risk was increased among MRSA nasal carriers compared with methicillin-susceptible S. aureus carriers and noncarriers, but risk differential diminished over time.
Current status and perspective of CAR-T and CAR-NK cell therapy trials in Germany (2021)

Chimeric antigen receptor (CAR)-T cell therapies are on the verge of becoming powerful immunotherapeutic tools for combating hematological diseases confronted with pressing medical needs. Lately, CAR-NK cell therapies have also come into focus as novel therapeutic options to address hurdles related to CAR-T cell therapies, such as therapy-induced side effects. Currently, more than 500 CAR-T and 17 CAR-NK cell trials are being conducted worldwide including the four CAR-T cell products Kymriah, Yescarta, Tecartus and Breyanzi, which are already available on the market. Most CAR-T cell-based gene therapy products that are under clinical evaluation consist of autologous enriched T cells, whereas CAR-NK cell-based approaches can be generated from allogeneic donors. Besides modification based on a second-generation CAR, more advanced CAR-immune cell therapeutics are being tested, which utilize precise insertion of genes to circumvent graft-versus-host disease (GvHD) or employ a dual targeting approach and adapter CARs in order to avoid therapy resistance caused by antigen loss. In this review, we are going to take a closer look at the commercial CAR-T cell therapies, as well as on CAR-T and CAR-NK cell products, which are currently under evaluation in clinical trials, that are being conducted in Germany.
MFIX documentation theory guide (1993)

This report describes the MFIX (Multiphase Flow with Interphase exchanges) computer model. MFIX is a general-purpose hydrodynamic model that describes chemical reactions and heat transfer in dense or dilute fluid-solids flows, flows typically occurring in energy conversion and chemical processing reactors. MFIX calculations give detailed information on pressure, temperature, composition, and velocity distributions in the reactors. With such information, the engineer can visualize the conditions in the reactor, conduct parametric studies and what-if experiments, and, thereby, assist in the design process. The MFIX model, developed at the Morgantown Energy Technology Center (METC), has the following capabilities: mass and momentum balance equations for gas and multiple solids phases; a gas phase and two solids phase energy equations; an arbitrary number of species balance equations for each of the phases; granular stress equations based on kinetic theory and frictional flow theory; a user-defined chemistry subroutine; three-dimensional Cartesian or cylindrical coordinate systems; nonuniform mesh size; impermeable and semi-permeable internal surfaces; user-friendly input data file; multiple, single-precision, binary, direct-access, output files that minimize disk storage and accelerate data retrieval; and extensive error reporting. This report, which is Volume 1 of the code documentation, describes the hydrodynamic theory used in the model: the conservation equations, constitutive relations, and the initial and boundary conditions. The literature on the hydrodynamic theory is briefly surveyed, and the bases for the different parts of the model are highlighted.
Does no‐till farming induce water repellency to soils? (2010)

Abstract Soil water repellency (SWR) is an intrinsic and dynamic soil property that can influence soil hydrology and crop production. Although several land use systems have been shown to induce water repellency in soil, the specific effects of no‐till cropping on SWR are poorly understood. This article reviews the impacts of no‐till on SWR and identifies research needs. No‐till cropping generally induces 1.5 to 40 times more SWR than conventional tillage, depending on soil type. This may result from near‐surface accumulation of hydrophobic organic C compounds derived from crop residues, microbial activity and reduced soil disturbance. While large SWR may have adverse impacts on soil hydrology and crop production, the level of SWR under no‐till relative to conventional tillage may contribute to aggregate stabilization and intra‐aggregate C sequestration. More research is needed to discern the extent and relevance of no‐till induced SWR. This includes: (1) further assessment of SWR under different tillage systems across a wide range of soil textures and climates, (2) comparison of the various methods for measuring SWR over a range of water contents, (3) inclusion of SWR in routine soil analysis and its use as a parameter to evaluate management impacts, (4) assessment of the temporal and spatial changes in SWR under field conditions, (5) further assessment of the impacts of the small differences in SWR between no‐till and conventionally tilled soils on crop production, soil hydrology and soil C sequestration, and (6) development of models to predict SWR for different tillage systems and soils.
A New Optics for Dark-Field Imaging in X-Ray Region `Owl' (2001)

An X-ray optics for X-ray dark-field imaging named `Owl' is proposed. It involves an asymmetric monochromator and a monolithic X-ray optics comprising a collimator C and an analyzer A. The C and A have 4,4,0 diffraction and thickness of 1.08 mm±10 µm. The X-ray energy used was 35 keV. These diffractions have an angular width of approximately 0.3 µrad. The analyzer A accepts only the refracted component from a sample S which is located between C and A, while eliminating the straight component without reacting with it. Two samples, one Al matrix containing boron fibers of 300 µm diameter and the other a wax block containing a nylon fiber simulating breast cancer, show very clear fiber imaging usually not visible by the conventional X-ray technique.
Echocardiographic Lesion Characteristics Associated with Successful Ablation of Inappropriate Sinus Tachycardia (2001)

An echocardiographically guided anatomic approach to RF ablation of inappropriate sinus tachycardia is safe and effective. A characteristic echocardiographic signature suggesting transmural/epicardium damage appears to be present at the time of successful heart rate reduction and may serve as an appropriate guide for directing additional RF when using this anatomic echocardiographically based approach.
FORCES UNDER THE FOOT (1973)

1. A method is described for measurement of the vertical load carried by different areas of the foot while walking barefoot. The results of tests on fourteen subjects, seven with some foot abnormality, are reported. 2. The results show that the load carried by the normal midfoot is low. Measurements in this area could be useful in the quantitative assessment of some foot abnormalities. 3. Considerable variation between individuals is seen in the distribution of vertical load across the forefoot. The metatarsal bones do not necessarily carry loads in proportion to their size.
Identification, cloning, and nucleotide sequence of a silent S-layer protein gene of Lactobacillus acidophilus ATCC 4356 which has extensive similarity with the S-layer protein gene of this species (1995)

The bacterial S-layer forms a regular structure, composed of a monolayer of one (glyco)protein, on the surfaces of many prokaryotic species. S-layers are reported to fulfil different functions, such as attachment structures for extracellular enzymes and major virulence determinants for pathogenic species. Lactobacillus acidophilus ATCC 4356, which originates from the human pharynx, possesses such an S-layer. No function has yet been assigned to the S-layer of this species. Besides the structural gene (slpA) for the S-layer protein (S-protein) which constitutes this S-layer, we have identified a silent gene (slpB), which is almost identical to slpA in two regions. From the deduced amino acid sequence, it appears that the mature SB-protein (44,884 Da) is 53% similar to the SA-protein (43,636 Da) in the N-terminal and middle parts of the proteins. The C-terminal parts of the two proteins are identical except for one amino acid residue. The physical properties of the deduced S-proteins are virtually the same. Northern (RNA) blot analysis shows that only the slpA gene is expressed in wild-type cells, in line with the results from sequencing and primer extension analyses, which reveal that only the slpA gene harbors a promoter, which is located immediately upstream of the region where the two genes are identical. The occurrence of in vivo chromosomal recombination between the two S-protein-encoding genes will be described elsewhere.
Recognition Requirements for the Activation, Differentiation and Function of T‐Helper Cells Specific for Class I MHC Alloantigens (1987)

The present review has focused on the specificity of the T-helper cell populations initiating MHC class I alloreactions. In contrast to conventional immune responses against soluble antigens, responses against membrane-bound class I alloantigens are initiated by two distinct antigen-specific T-helper cell populations that can be distinguished by their Lyt phenotype, MHC restriction specificity, antigen specificity, and requirement for thymically determined self-recognition. Alloresponses were shown to be a composite consisting of two distinct components: one mediated by L3T4+ Th cells and very similar to conventional self + X responses; and one mediated by Lyt2+ Th cells and unique to alloresponses against MHC class I antigens. As would befit an unusual Th cell population, the recognition/response spectrum of Lyt2+ Th cells was highly unusual and was found to be the basis for much of the uniqueness we attribute to immune alloreactions, including rapid rejection of tissue allografts in vivo.
VEGF–PLCγ1 pathway controls cardiac contractility in the embryonic heart (2005)

The strength of the heart beat can accommodate in seconds to changes in blood pressure or flow. The mechanism for such homeostatic adaptation is unknown. We sought the cause of poor contractility in the heart of the embryonic zebrafish with the mutation dead beat. We find through cloning that this is due to a mutation in the phospholipase C gamma1 (plcgamma1) gene. In mutant embryos, contractile function can be restored by PLCgamma1 expression directed selectively to cardiac myocytes. In other situations, PLCgamma1 is known to transduce the signal from vascular endothelial growth factor (VEGF), and we show here that abrogation of VEGF also interferes with cardiac contractility. Somewhat unexpectedly, FLT-1 is the responsible VEGF receptor. We show that the same system functions in the rat. Blockage of VEGF-PLCgamma1 signaling decreases calcium transients in rat ventricular cardiomyocytes, whereas VEGF imposes a positive inotropic effect on cardiomyocytes by increasing calcium transients. Thus, the muscle of the heart uses the VEGF-PLCgamma1 cascade to control the strength of the heart beat. We speculate that this paracrine system may contribute to normal and pathological regulation of cardiac contractility.
Collaborative Multi-Object Tracking as an Edge Service using Transfer Learning (2020)

Video cameras have been pervasively deployed in Smart Cities and timely processing of the huge amount of video data brings up new challenges. Each individual camera is only able to cover a limited range due to the effective monitoring distances and the complicated layout of a building or an area. Once the object of interest moves away from the camera field of view, the system may lose object tracking. When the object appears and is captured by another camera, object detection, feature abstraction, and behavior classification require an association with other video data exploitation results. Hence, pragmatic approaches for object re-verification (or re-acquisition) are needed so as to not infer the object as a new track. In this paper, we propose a novel Collaborative Multi-Object Tracking system as an Edge service (CoMOTE) based on transfer learning technology. A transfer learning-based human detection and tracking method is introduced, which allocates most of the computing tasks on edge devices. An edge server is adopted to integrate information from edge cameras for decision-making to enable multi-view surveillance. The CoMOTE scheme not only reduces the dependence on a central server but also leads to a lower communication cost. Implemented on edge nodes (Raspberry Pi 4) and tested with WildTrack multi-view video database, extensive experimental results verify that the CoMOTE prototype achieved the design goal and outperforms multiple state-of-the-art methods.
The Effect of Capacity, Rotational Speed and Storage on Crystallization and Rheological Properties of Puff Pastry Butter (2013)

Abstract In the margarine and butter industry, sustainable and more efficient refrigerants, such as CO 2 , are introduced to industrial scraped surface heat exchangers, allowing an increased capacity compared to conventional use of NH 3 . The effect of such changes in capacity and a varied rotational speed was studied in relation to the structural behavior of puff pastry butter during 4 weeks of isothermal storage at 20 °C. The physical properties of the fat crystal network were studied in detail at several length scales by combining X‐ray diffraction with differential scanning calorimetry, confocal laser scanning microscopy, LR‐NMR and rheology. Our data shows that a high capacity combined with high rotational speed decreases the brittleness of puff pastry butter after 7 days of storage. This effect is, however, diminished after 28 days of storage. Likewise, changes in capacity and rotational speed are shown to induce no microstructural and polymorphic differences after 28 days of storage. However, the degree of work softening is related to the manufacturing conditions: a high capacity and a high rotational speed increase the ability of the puff pastry butter to resist structural breakdown during working. With this being the only observed difference; a wide operational window exists on an industrial level to produce puff pastry butter with similar structural behavior.
Nuclear Power from Thorium:Different Options (2016)

Thorium is a fertile material that has drawn attention as a potential source of nuclear energy since the 1950s due to several attractive features of the Th-U 233 fuel cycle. In view of the renewed interest in thorium, the possibilities of thorium utilization in different reactor systems, namely pressurized heavy water reactors (PHWRs), light water reactors, molten salt breeder reactors (MSBRs), fast reactors and accelerator-driven sub-critical systems have been examined. Extraction of energy from thorium essentially requires prior conversion of thorium to fissile U 233 . For in situ burning of thorium, a high burn-up is therefore essential. It is shown that the use of thorium in currently deployed PHWRs will reduce the requirement of uranium by about 30% in once through fuel cycle, while MSBRs with closed fuel cycle can achieve near breeding capability in thermal reactors. The most effective thorium utilization can be achieved only by adopting a closed fuel cycle which will not only enhance the fissile inventory many fold but also reduce nuclear waste burden significantly. While in conventional fast breeder reactors, thorium, partly converted into U 233 in the blanket region, is reprocessed for the recovery of the fissile material; in the breed and burn concept, the converted material is transferred to the core region without any reprocessing. Availability of spallation neutrons produced by bombardments of high-energy protons on heavy nuclides can augment fertile to fissile conversion leading to thorium utilization. The various options, which appear technologically feasible for generating power from thorium and the key issues connected with these schemes, are discussed in this article.