gebhart/patrepeval huggingface dataset:
Hypoxia-induced epigenetic modifications are associated with cardiac tissue fibrosis and the development of a myofibroblast-like phenotype (2013)

Ischemia caused by coronary artery disease and myocardial infarction leads to aberrant ventricular remodeling and cardiac fibrosis. This occurs partly through accumulation of gene expression changes in resident fibroblasts, resulting in an overactive fibrotic phenotype. Long-term adaptation to a hypoxic insult is likely to require significant modification of chromatin structure in order to maintain the fibrotic phenotype. Epigenetic changes may play an important role in modulating hypoxia-induced fibrosis within the heart. Therefore, the aim of the study was to investigate the potential pro-fibrotic impact of hypoxia on cardiac fibroblasts and determine whether alterations in DNA methylation could play a role in this process. This study found that within human cardiac tissue, the degree of hypoxia was associated with increased expression of collagen 1 and alpha-smooth muscle actin (ASMA). In addition, human cardiac fibroblast cells exposed to prolonged 1% hypoxia resulted in a pro-fibrotic state. These hypoxia-induced pro-fibrotic changes were associated with global DNA hypermethylation and increased expression of the DNA methyltransferase (DNMT) enzymes DNMT1 and DNMT3B. Expression of these methylating enzymes was shown to be regulated by hypoxia-inducible factor (HIF)-1α. Using siRNA to block DNMT3B expression significantly reduced collagen 1 and ASMA expression. In addition, application of the DNMT inhibitor 5-aza-2'-deoxycytidine suppressed the pro-fibrotic effects of TGFβ. Epigenetic modifications and changes in the epigenetic machinery identified in cardiac fibroblasts during prolonged hypoxia may contribute to the pro-fibrotic nature of the ischemic milieu. Targeting up-regulated expression of DNMTs in ischemic heart disease may prove to be a valuable therapeutic approach.
Direct VLSI Implementation of Combinatorial Algorithms (1979)

We present new algorithms for dynamic programming and transtivc closure which arc appropriate\nfor very large-scale integration implementation.
Fundamentals and applications of spectroscopic ellipsometry (2002)

This paper describes the use of ellipsometry as a precise and accurate technique for characterizing substrates and overlayers. A brief historical development of ellipsometry and the basic principles necessary to understand how an ellipsometer works are presented. There are many examples of studies performed in addressing materials science issues, and several are presented here: measurements of thickness, optical properties, and modeling of surface roughness. These selected results obtained in our laboratory for substrates, Si/SiO2 interfaces, and polymers provide evidence that ellipsometry can play a critical role in characterizing different types of materials.
Forward error correction for CELP encoded speech (2002)

Communication systems use low-rate vocoders to reduce the bandwidth requirements of transmitted speech. However, these algorithms introduce a new problem in that the quality of the reconstructed voice exhibits a much greater sensitivity to compressed parameter bit errors than it would to sampled speech bit errors. Furthermore, within each compressed packet, individual bits vary widely as to their contribution to overall speech quality. This paper analyzes the bit-error performance of CELP encoded speech and develops a forward error correction technique which maximizes speech quality using a given number of error correction bits.
The influence of a residual group in low‐molecular‐weight allergoids of <i>Artemisia vulgaris</i> pollen on their allergenicity, IgE‐ and IgG‐binding properties (2002)

According to our results, all three modification procedures yielded allergoids with a similar extent of modification. No single biochemical parameter investigated in the study could predict the degree of reduced allergenicity in vivo. The most reduced allergenicity was seen in the succinyl derivative while the preservation of IgG binding epitopes was of the highest degree for the carbamyl derivative.
Effect of salts and sugars on the clarity of gellan gels (2011)

Summary The effect of glucose, fructose and sucrose (0–15 wt%) on the clarity of low acyl gellan gels (0.5 wt%) in the presence of potassium chloride (40–100 m m ), calcium chloride (10–80 m m ) and their mixtures at a total molar concentration of 80 m m , was investigated by light absorbance experiments (490 nm). The effect on gel clarity (at 100 m m KCl) of all sugar combinations, at a final concentration of 15 wt%, was also investigated. All gels with potassium were less turbid than the ones with calcium. In the case of salt mixtures, increasing concentration of calcium in the mixtures resulted in decreased clarity. In addition, turbidity increased with increasing salt concentration. Moreover, and at the higher salt concentrations, the presence of sugars resulted in clearer gels. When mixtures of sugars were added, all combinations resulted in increased clarity compared to the individual sugars.
Effects of islet neogenesis-associated protein pentadecapeptide on cell mass and insulin secretion of pancreatic β-cells. (2012)

INGAP-PP increased the insulin secretion, enhanced the proliferation and might reduce apop tosis of β-cells. The mechanism may contribute to the changed expression of some genes related to cell cycle.
Precise Control of Cell Adhesion by Combination of Surface Chemistry and Soft Lithography (2012)

The adhesion of cells on an extracellular matrix (ECM) (in vivo) or the surfaces of materials (in vitro) is a prerequisite for most cells to survive. The rapid growth of nano/microfabrication and biomaterial technologies has provided new materials with excellent surfaces with specific, desirable biological interactions with their surroundings. On one hand, the chemical and physical properties of material surfaces exert an extensive influence on cell adhesion, proliferation, migration, and differentiation. On the other hand, material surfaces are useful for fundamental cell biology research and tissue engineering. In this Review, an overview will be given of the chemical and physical properties of newly developed material surfaces and their biological effects, as well as soft lithographic techniques and their applications in cell biology research. Recent advances in the manipulation of cell adhesion by the combination of surface chemistry and soft lithography will also be highlighted.
Melatonin-lnduced Decrease of Body Temperature in Women: A Threshold Event (1994)

Whether the biological effect of melatonin in humans is directly related to the circulating levels of the hormone, has not heretofore been investigated. In this study, we investigated whether previously described hypothermic melatonin properties are dose related. The nocturnal decline of the body temperature (BT) observed in 16 early follicular phase women, following placebo administration at 18.00 h, was compared with that observed during the preceding or following night, after melatonin suppression with the beta 1-adrenergic antagonist atenolol (100 mg). In 6 subjects (37.5%) with lower nocturnal melatonin levels (p < 0.05) atenolol induced a complete melatonin suppression and an attenuation of the nocturnal BT decline (p < 0.02), whereas in the remaining 10 subjects (62.5%) atenolol induced an incomplete melatonin suppression with no modification of the nocturnal BT decline. During a 3rd night, 2 of the 6 subjects with complete and 6 of the 10 subjects with incomplete melatonin suppression blindly received atenolol plus melatonin (1 mg at 19.30 h and 0.75 mg at 21.00 and 23.00 h). Exogenous melatonin restored the full expression of the nocturnal BT decline in the 2 subjects with complete melatonin suppression, but did not modify the BT decline in the 6 subjects with atenolol-induced incomplete melatonin suppression. Our data show that markedly, but not completely attenuated nocturnal melatonin levels are sufficient to exert maximal thermoregulatory effects, indicating rather a threshold than a dose-response effect of melatonin action on human BT.
Gene expression pattern of the epidermal growth factor receptor family and LRIG1 in renal cell carcinoma (2012)

This study revealed that the previously described up-regulation of EGFR and down-regulation of ERBB4 occurred in all analyzed RCC types, whereas down-regulation of ERBB2 and LRIG1 was only present in ccRCC. These observations illustrate the need to evaluate the different RCC types individually when analyzing molecules of interest and potential biological markers.
Gray scale features of hematomas: an ultrasonic spectrum (1978)

Forty-eight B-scan ultrasound examinations were performed on 25 patients with hematomas. Hematomas are usually spherical or ovoid within the abdomen, and lentiform within the pleural space or abdominal wall. They generally decrease in size with time, and usually have irregular walls. Hematomas contain a variable amount of internal echoes during the first month, and then gradually become anechoic. The sonographic appearance is not specific; an abscess may have a similar appearance. An old, anechoic hematoma may contain a gelatinous material which cannot be aspirated.
For the Analysis of Propaganda (1938)

FOR THE ANALYSIS OF PROPAGANDA Get access CLYDE R. MILLER CLYDE R. MILLER Search for other works by this author on: Oxford Academic Google Scholar Public Opinion Quarterly, Volume 2, Issue 1, January 1938, Pages 133–134, https://doi.org/10.1086/265163 Published: 01 January 1938
One-pass encoding of connected components in multivalued images (2002)

A method of representing connected components in multivalued images is proposed, and an efficient, sequential, one-pass algorithm for generating the border-line chain code for each component is introduced. Simultaneously, the algorithm produces for each component a list of all neighboring components that are encountered during a walk around the border line, thus providing full topological information. The number of different pixel values does not affect performance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Evaluation of an Active Safety Light using Virtual Test Drive within Vehicle in the Loop (2010)

Driving at night time increases the risk of accidents because the driver's perception is reduced to the limited area of the environment which is illuminated by the car's head lights. In the study presented in this paper, the tool Vehicle in the Loop (VIL) is used the first time to research the potential of a new light function. VIL is a Virtual Reality application and simulation setup which allows for testing driver assistance systems in critical driving situations while driving a real car, but without the risk of collisions with real objects. VIL is based on the Virtual Test Drive (VTD) simulation software. The Active Safety Light is an advanced light functionality which illuminates the potential escape path a driver should take in order to avoid an imminent accident. The two main results of this first field study are, that VIL is suitable to study advanced light functions and that the Active Safety Light supports the driver in critical situations. The driver reacts nearly 60 ms faster and the situation is estimated as more critical so that the driver brakes instinctively harder, i.e. the break pressure is increased by almost a factor of two.
The dielectric-filled parabola: a new high frequency integrated receiver or transmitter front end (2003)

A design is presented for a high-frequency front end that can be used as a receiver or transmitter, in a heterodyne or direct detection mode, as a single device or in conjunction with a focal-plane array. The novel feed system combines the simplicity and robustness of a dielectric substrate lens with the high gain of a parabolic reflector in a single structure, the dielectric parabola. The design is easy to fabricate, can be scaled to very high frequencies, and is cryogenically coolable. Beam patterns and antenna input impedances have been measured for a number of different planar antenna elements on the dielectric-filled parabola from 4 to 12 GHz. The results of these measurements show clearly that the system performs well.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Real-Time Planning for Multiple Autonomous Vehicles in Dynamic Uncertain Environments (2004)

In a highly dynamic environment, an adaptive real-time mission planner is essential for controlling a team of autonomous vehicles to execute a set of tasks. An optimal plan computed prior to the operation will no longer be optimal when the vehicles execute the plan. This dissertation presents a framework and algorithms for solving real-time task and path planning problems by combining Evolutionary Computation (EC) based techniques with a Market-based planning architecture. The planning system takes advantage of the flexibility of EC-based techniques and the distributed structure of Market-based planning. This property allows the vehicles to evolve their task plans and routes in response to the changing environment in real time, and under varying computational time windows.* 
*This dissertation is a compound document (contains both a paper copy and a CD as part of the dissertation). The CD requires the following system requirements: Windows MediaPlayer or RealPlayer.
High-density 16/8/4-bit configurable multiplier (1997)

A configurable serial–parallel multiplier based on Braun's and Baugh–Wooley's algorithms is presented. The multiplier can be configured to perform either signed or unsigned multiplications and to achieve variable precision. In this device one factor A(m) is fed serially with an arbitrary wordlength m while the other B(n) is stored in parallel with a configurable number of bits n = 4, 8 or 16 bits. Switch elements are used to change the hardware connection between adjacent 4-bit multiplier basic blocks. This reconfiguration concept provides a higher precision multiplier by grouping adjacent cells or a higher throughput at low levels of precisions. A prototype of this multiplier has been fabricated using a full custom 1.0 µm CMOS technology. The active area contains 3450 transistors and occupies 0.47 mm2 corresponding to a very high gate density of 1532 gates/mm2.
Monoclonal antibodies to Actinobacillus actinomycetemcomitans (1988)

Murine hybridoma cell lines were developed which synthesized monoclonal antibodies against Actinobacillus actinomycetemcomitans-associated antigens. Monoclonal antibodies specific for an antigen(s) common to all A. actinomycetemcomitans isolates tested but not detected on other gram-negative oral plaque microorganisms or other Actinobacillus species were identified. Monoclonal antibodies specific for each serotype group of A. actinomycetemcomitans which did not bind to other Actinobacillus species or oral plaque microorganisms were also identified.
The Proton-Pump Inhibitor Lansoprazole Enhances Amyloid Beta Production (2013)

A key event in the pathogenesis of Alzheimer's disease (AD) is the accumulation of amyloid-β (Aβ) species in the brain, derived from the sequential cleavage of the amyloid precursor protein (APP) by β- and γ-secretases. Based on a systems biology study to repurpose drugs for AD, we explore the effect of lansoprazole, and other proton-pump inhibitors (PPIs), on Aβ production in AD cellular and animal models. We found that lansoprazole enhances Aβ37, Aβ40 and Aβ42 production and lowers Aβ38 levels on amyloid cell models. Interestingly, acute lansoprazole treatment in wild type and AD transgenic mice promoted higher Aβ40 levels in brain, indicating that lansoprazole may also exacerbate Aβ production in vivo. Overall, our data presents for the first time that PPIs can affect amyloid metabolism, both in vitro and in vivo.
Cell Adhesion Assays (2011)

Standard adhesion assays measure cell binding either to immobilized ligands or to cell monolayers in flat-well microtiter plates under static conditions. Typically, these test systems require several washing steps to separate adherent from nonadherent cells. Here, we describe an adhesion assay which avoids these washing steps by employing V-bottom 96-well plates. In this assay, fluorescently labeled leukocytes are allowed to adhere to V-well plates coated with soluble ligand for a fixed time. Thereafter, centrifugal force is applied to separate adherent cells from nonadherent cells. Nonadherent cells accumulate in the nadir of the V-shaped wells and are quantified using a fluorometer with a narrow aperture. This simple and reproducible method has been validated with different classes of adhesion molecule families (selectins and integrins) and is adaptable to several other adhesive interactions. The assay format is suitable for screening applications and may also be used for diagnostic testing. The receptor/ligand interaction chosen as an example to describe the assay methodology is the interaction between the integrin lymphocyte function-associated molecule-1 (LFA-1, αLβ2) and intercellular adhesion molecule-1 (ICAM-1).
Glutathione as a treatment for male infertility (1996)

The excessive generation of reactive oxygen species (ROS) by abnormal spermatozoa and by contaminating leukocytes has been identified as one of the few defined aetiologies for male infertility. As a consequence, work has begun on evaluating the role of antioxidants in the management of these patients. Glutathione plays a significant role in the antioxidant defences of the spermatogenic epithelium, the epididymis, and perhaps in ejaculated spermatozoa. The use of antioxidants in vitro appears to be of value in preserving fertilizing capacity, although no clinical data are available. Glutathione administered in vivo to patients who may have infertility secondary to excessive oxidative stress appears to act at the epididymis and during spermatogenesis, to improve the function of ejaculated spermatozoa. However, fertility studies have not yet been conducted. Controlled studies of glutathione and other antioxidants in patients with defined ROS pathology are urgently required.
METHOD FOR BROADENING THE BEAMWIDTHS OF CROSSED DIPOLE FOR WIDEBAND MARINE GPS APPLICATIONS (2009)

In this paper, a method to broaden the beamwidths of a crossed dipole antenna is proposed. By introducing four parasitic strips around the crossed dipole antenna, the beamwidths of the crossed dipole antenna in the vertical plane are broadened efiectively, making the patterns uniform over a wide frequency band. An L-band prototype, operating
Size- and Structure-Controlled Synthesis and Characterization of Nickel Nanoparticles (2009)

Nearly monodisperse face-centered cubic (fcc) and hexagonal close-packed (hcp) nickel nanoparticles have been prepared via the thermal decomposition of nickel organometallic precursors in a reaction mixture containing alkylamine, and characterized by powder X-ray diffraction, transmission electron microscopy and magnetic measurement. The employed alkylamine can serve as both solvent and reducing agent. The as-synthesized nickel nanoparticles are air-stable, and their sizes can be readily tuned by the variation of surfactant concentration and reaction temperature. The crystalline phase control was achieved by adjusting alkylamine concentration, heating rate and reaction temperature. Magnetic measurements showed that hcp Ni nanoparticles have quite different magnetic properties compared to fcc Ni nanoparticles.
Crosstalk between SDF-1/CXCR4 and SDF-1/CXCR7 in cardiac stem cell migration (2015)

Stromal cell-derived factor 1 (SDF-1) is a chemokine that can be expressed in injured cardiomyocytes after myocardial infarction (MI). By combining with its receptor CXCR4, SDF-1 induced stem and progenitor cells migration. CXCR7, a novel receptor for SDF-1, has been identified recently. We aimed to explore the roles of SDF-1/CXCR4 and SDF-1/CXCR7 pathway and their crosstalk in CSCs migration. In the present study, CXCR4 and CXCR7 expression were identified in CSCs. Transwell assay showed that SDF-1 caused CSCs migration in a dose- and time-dependent manner, which could be significantly suppressed by CXCR4 or CXCR7 siRNA. Phospho-ERK, phospho-Akt and Raf-1 significantly elevated in CSCs with SDF-1 stimulation. Knockdown of CXCR4 or CXCR7 significantly decreased phospho-ERK or phospho-Akt, respectively, and eventually resulted in the inhibition of CSCs migration. Moreover, western blot showed that MK2206 (Akt inhibitor) increased the expression of phospho-ERK and Raf-1, whereas PD98059 (ERK inhibitor) had no effect on phospho-Akt and Raf-1. GW5074 (Raf-1 inhibitor) upregulated the expression of phospho-ERK, but had no effect on phospho-Akt. The present study indicated that SDF-1/CXCR7/Akt and SDF-1/CXCR4/ERK pathway played important roles in CSCs migration. Akt phosphorylation inhibited Raf-1 activity, which in turn dephosphorylated ERK and negatively regulated CSCs migration.
Evaluation of Immediate and Short-Term Efficacy of DualStim Therapy with and without Intracavernosal Umbilical Cord-Derived Wharton’s Jelly in Patients with Erectile Dysfunction: Study Protocol for a Pilot Randomised Controlled Trial (2020)

Abstract Background Erectile Dysfunction (ED) affects a significant portion of the U.S. population and causes negative psychological burdens that affects men and their partner’s quality of life and satisfaction. Extracorporeal shock therapy (ESWT) utilizing focused ESWT (fESWT) and radial ESWT (rESWT) in Low-intensity shock wave therapy (LSIWT) has been used to treat ED with some success. Wharton’s Jelly (WJ) is a biologic substance with large amounts of stem cells, growth factors, cytokines and extracellular components. The use of simultaneous fESWT and rESWT (DualStim Therapy) combined with WJ have potential uses in ED that may have advantages over current treatments. Methods A randomized, single-blinded, controlled clinical trial will be conducted to evaluate the efficacy and safety of DualStim Therapy and ICIs of WJ in moderate to severe ED. A total of 60 patients with moderate to severe ED will be enrolled and treated with DualStim Therapy with intracavernosal injection (ICIs) of WJ or saline for a period of 7 weeks. The International Index of Erectile Function – Erectile Function (IIEF-EF) score will be used to gauge the treatment related changes in relation to the subject’s baseline. The scores will be recorded at baseline and compared to follow-ups 1,3 and 6 months post-treatment. Any Adverse events (AEs) or severe AEs will be recorded in the corresponding case report forms (CRFs). Sexual Encounter Profile (SEP), as well as the Global Assessment Questionnaire (GAQ) and the Erection Hardness Score (EHS) will be used to determine the sexual activity improvement from baseline leading to optimal penetration at follow-ups 1,3 and 6 months post-treatment. Discussion This clinical trial is a feasibility study to determine the immediate and short-term efficacy of DualStim Therapy, with and without ICIs of formulated umbilical cord-derived WJ to improve and/or restore erectile function in patients with moderate to severe ED. This study will also provide insight into the safety and efficacy of WJ. We anticipate clinically significant improvement in patients suffering from moderate and severe ED treated with DualStim therapy with WJ compared to their baseline and DualStim with saline. Trial registration Registered on ClinicalTrials.gov; the trial number is NCT04424394
An Optimally Controlled Respirator (1971)

An optimally controlled respirator was developed. It has three main features: 1) ventilation is controlled by the patient's metabolic rate from continuously measured C0 <L xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</L> output, 2) physiologic dead space approximated as a linear function of tidal volume is used to estimate alveolar ventilation, and 3) respiratory rate is computed to minimize ventilatory work.