gebhart/patrepeval huggingface dataset:
REACTIVE OXYGEN SPECIES, NRF2 SIGNALING, AND HUMAN AGING AND DISEASES (2018)

Reactive oxygen species (ROS) are the underlying etiology of aging and many aging-related diseases. Our body has been equipped with many systems to cope with the production of ROS or oxidative stress. NRF2, a transcription factor, is critical for maintaining our body’s redox homeostasis by signaling the production of antioxidants and detoxifying enzymes. However, the basal level of NRF2 decreases as we age, which results in increased diseases in the aged. My laboratory has worked for many years to understand how NRF2 is regulated and to develop natural products or small synthetic molecules that activate the Nrf2 pathway for disease prevention. NRF2 activators have proven to be useful in protecting against many diseases both in rodent models and in human clinical trials. In this talk, I will present results from my laboratory that demonstrate the functional role of NRF2 in aging and human diseases (Parkinson’s disease and cancer).
The Synthesis of Diamond Films at Lower Pressure and Lower Temperature Using Magneto-Microwave Plasma CVD (1989)

Diamond films have been obtained using magneto-microwave plasma assisted CVD at 0.1 Torr. The reaction gas is a CO/H 2 mixture. By setting the ECR condition at the deposition area, a high density plasma (1×10 11 cm -3 ) is obtained around the substrate. The discharge area is quite uniform in the pressure. Diamond films are obtained on positively biased substrates. As the reaction pressure becomes lower, the substrate temperature for the diamond formation tends to decrease. The films were evaluated by SEM imaging, electron diffraction and Raman spectroscopy.
Cell-mediated immunity to <i>Malassezia furfur </i>in patients with seborrhoeic dermatitis and pityriasis versicolor (1999)

The lymphocyte transformation response to Malassezia furfur, Candida albicans, phytohaemagglutinin, concanavlin A and tuberculin purified protein derivative of 12 patients with pityriasis versicolor, 15 patients with seborrhoeic dermatitis and matched controls, was studied. Patients with pityriasis versicolor showed a significantly lower response to M. furfur than patients with seborrhoeic dermatitis and controls.
Imaging of Vx‐2 rabbit tumors with α<sub>ν</sub>β<sub>3</sub>‐integrin‐targeted <sup>111</sup>In nanoparticles (2007)

Earlier tumor detection can improve 5-year survival of patients, particularly among those presenting with cancers less than 1 cm in diameter. alpha(nu)beta(3)-Targeted (111)In nanoparticles (NP) were developed and studied for detection of tumor angiogenesis. Studies were conducted in New Zealand white rabbits implanted 12 days earlier with Vx-2 tumor. alpha(nu)beta(3)-Targeted (111)In/NP bearing approximately 10 (111)In/NP vs. approximately 1 (111)In/NP nuclide payloads were compared to nontargeted radiolabeled control particles. In vivo competitive binding studies were used to assess ligand-targeting specificity. alpha(nu)beta(3)-Integrin-targeted NP with approximately 10 (111)In/NP provided better (p < 0.05) tumor-to-muscle ratio contrast (6.3 +/- 0.2) than approximately 1 (111)In/NP (5.1 +/- 0.1) or nontargeted particles with approximately 10 (111)In/NP (3.7 +/- 0.1) over the initial 2-hr postinjection. At 18 hr, mean tumor activity in rabbits receiving alpha(nu)beta(3)-integrin-targeted NP was 4-fold higher than the nontargeted control. Specificity of the NP for the tumor neovasculature was supported by in vivo competition studies and by fluorescence microscopy of alpha(nu)beta(3)-targeted fluorescent-labeled NP. Biodistribution studies revealed that the primary clearance organ in rabbits as a %ID/g tissue was the spleen. Circulatory half-life (t(1/2)beta) was estimated to be approximately 5 hr using a 2-compartment model. alpha(nu)beta(3)-Targeted (111)In perfluorocarbon NP may provide a clinically useful tool for sensitively detecting angiogenesis in nascent tumors, particularly in combination with secondary high-resolution imaging modalities, such as MRI.
Size and Performance Tradeoffs in Micro-Inductors for High Frequency DC-DC Conversion (2009)

This paper presents the design and measurement of optimized inductors-on-silicon (ldquomicro-inductorsrdquo) for use in high frequency DC-DC conversion applications. These ultra-compact low profile (< 70 mum) inductors range in area from 0.5-2.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The design process utilizes optimization software that finds the optimal physical dimensions, to maximize inductor efficiency, given certain technology constraints. Optimized micro-inductors achieve measured inductance densities of 66 nH/mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for a 2.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> area, 110 nH/mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for 1.3 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> device and 82 nH/mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for a 0.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> footprint area. Measured saturation currents range from 0.15-0.5 A for the optimized devices.
Atomistic simulation of the size and orientation dependences of thermal conductivity in GaN nanowires (2007)

The thermal conductivity of GaN nanowires has been determined computationally by applying nonequilibrium atomistic simulation methods using the Stillinger-Weber [Phys. Rev. B 31, 5262 (1985)] potentials. The simulation results show that the thermal conductivity of the GaN nanowires is smaller than that of a bulk crystal and increases with increasing diameter. Surface scattering of phonons and the high surface to volume ratios of the nanowires are primarily responsible for the reduced thermal conductivity and its size dependence behavior. The thermal conductivity is also found to decrease with increasing temperature and exhibits a dependence on axial orientation of the nanowires.
Characterization of B1(Fv)PE38 and B1(dsFv)PE38: single-chain and disulfide-stabilized Fv immunotoxins with increased activity that cause complete remissions of established human carcinoma xenografts in nude mice. (1995)

The mAb B1 (mouse IgG1 kappa) recognizes a carbohydrate epitope on human carcinoma cells (I. Pastan et al., Cancer Res., 51: 3781-3787, 1991). We have generated plasmids encoding immunotoxins in which the Fv domain of B1, either as a single-chain Fv or as a disulfide-stabilized Fv (dsFv), was fused to PE38, a truncated form of Pseudomonas exotoxin A. To compare the activities of the two types of recombinant immunotoxins, the proteins were prepared from cytoplasmic inclusion bodies produced in Escherichia coli. The immunotoxins were evaluated for stability, antigen binding, specific cytotoxicity, pharmacokinetics, and antitumor activity in a nude mouse model. Although the single-chain immunotoxin is relatively stable when incubated at 37 degreesC (t(1/2) approximately 4 h), the dsFv immunotoxin is much more stable, with no loss of activity after 8 h at 37 degreesC. The single-chain immunotoxin has a 2-fold better binding affinity and cytotoxicity toward antigen-positive cultured cells than the dsFv immunotoxin. The half-lives in the blood of mice of B1(Fv)PE38 (single-chain) and B1(dsFv)PE38 (disulfide-stabilized) are 23 and 27 min, respectively. Their therapeutic potential was evaluated in athymic nude mice bearing human epidermoid carcinoma xenografts. Both immunotoxins caused complete regressions of the s.c. (30-40 mm3) tumors when given i.v. in three doses of 0.025 mg/kg every other day. This is one-twentieth of the mouse LD50. Recombinant immunotoxins containing the B1(Fv) are 2-3-fold more potent antitumor agents than previously described immunotoxins containing the B3(Fv) (Brinkmann et al., Proc. Natl. Acad. Sci. USA, 88: 8616-8620, 1991), which also target LeY and related carbohydrates in human tumors, but have a similar toxicity in mice. Thus, their therapeutic window is 2-3-fold larger. In addition, B1(dsFv)PE38 has only a 50% decrease in the apparent binding affinity of B1(Fv)PE38, whereas B3(dsFv)PE38 has a much greater loss in antigen binding.
Studies on the Structure and Function of Muscle Aldolase (1969)

Abstract In 0.05 m potassium phosphate buffer (pH 7.0, 20°), native rabbit muscle aldolase has been found to exhibit a molecular weight (Mn ∼ Mw ∼ Mz) close to 160,000, in agreement with previous findings. Upon exposure to cold alkaline borate buffer (pH 12.5, µ = 0.17, 0°), the enzyme spontaneously dissociates into its monomers which subsequently undergo slow hydrolytic degradation. Compensating for electrostatic charge effects and extrapolating the data to zero time in alkali, a subunit molecular weight of 41,400 to 42,000 is obtained. If the native enzyme is exposed to alkaline conditions at 20°, the subunits undergo an initial rapid degradation which eventually yields alkali-resistant material. Similar results are obtained with succinyl aldolase subunits under equivalent conditions. Acrylamide gel electrophoresis of alkali-treated succinyl aldolase (pH 12.5, 30°) results in the appearance of a limited number of electrophoretic bands. This finding suggests that specific alkali-labile peptide bonds are involved in the degradation process. As a further consequence of the action of alkali, native aldolase shows increased solubility properties. Both native and succinyl aldolase manifest new NH2-terminal serine, threonine, and glycine residues after treatment with borate buffer at pH 12.5 and 30°. Prolonged exposure to alkali results in increased liberation of the above end groups, but no evidence is obtained for other NH2-terminal residues except for a small amount of proline.
Correlation-based pattern recognition for implantable defibrillators. (1996)

An estimated 300,000 Americans die each year from cardiac arrhythmias. Historically, drug therapy or surgery were the only treatment options available for patients suffering from arrhythmias. Recently, implantable arrhythmia management devices have been developed. These devices allow abnormal cardiac rhythms to be sensed and corrected in vivo. Proper arrhythmia classification is critical to selecting the appropriate therapeutic intervention. The classification problem is made more challenging by the power/computation constraints imposed by the short battery life of implantable devices. Current devices utilize heart rate-based classification algorithms. Although easy to implement, rate-based approaches have unacceptably high error rates in distinguishing supraventricular tachycardia (SVT) from ventricular tachycardia (VT). Conventional morphology assessment techniques used in ECG analysis often require too much computation to be practical for implantable devices. In this paper, a computationally-efficient, arrhythmia classification architecture using correlation-based morphology assessment is presented. The architecture classifies individuals heart beats by assessing similarity between an incoming cardiac signal vector and a series of prestored class templates. A series of these beat classifications are used to make an overall rhythm assessment. The system makes use of several new results in the field of pattern recognition. The resulting system achieved excellent accuracy in discriminating SVT and VT.
Protein phosphorylation and the hormonal control of hepatic cholesterol synthesis (1983)

Conference Article| December 01 1983 Protein phosphorylation and the hormonal control of hepatic cholesterol synthesis THOMAS S. INGEBRITSEN THOMAS S. INGEBRITSEN 1Department of Pharmacology, University of Colorado Health Sciences Center, Denver, CO 80262, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1983) 11 (6): 644–646. https://doi.org/10.1042/bst0110644 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation THOMAS S. INGEBRITSEN; Protein phosphorylation and the hormonal control of hepatic cholesterol synthesis. Biochem Soc Trans 1 December 1983; 11 (6): 644–646. doi: https://doi.org/10.1042/bst0110644 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: HMG-CoA, 3-hydroxy-3-methylglutaryl-CoA This content is only available as a PDF. © 1983 Biochemical Society1983 Article PDF first page preview Close Modal You do not currently have access to this content.
7. More about the Regularity of Compactly Supported Wavelets (1992)

The regularity of the Meyer or the Battle—Lemarié wavelets is easy to assess: the Meyer wavelet has compact Fourier transform, so that it is , and the Battle-Lemarié wavelets are spline functions, more precisely, piecewise polynomial of degree k, with (k − 1) continuous derivatives at the knots. The regularity of compactly supported orthonormal wavelets is harder to determine. Typically, they have a non-integer Hölder exponent; moreover, they are more regular in some points than in others, as is already illustrated by Figure 6.3. This chapter presents a collection of tools that have been developed over the past few years to study the regularity of these wavelets. All of these techniques rely on the fact that ϕ(x)=∑cnϕ(2x−n),7.0.1 where only finitely many are nonzero; the wavelet ψ, as a finite linear combination of translates of ϕ(2x), then inherits the same regularity properties. It follows that the techniques exposed in this chapter are not restricted to wavelets alone; they apply as well to the basic functions in subdivision schemes (see §6.5). Some of the tools discussed here were in fact first developed for subdivision schemes, and not for wavelets.
<title>Computer-generated holograms and diffraction gratings in optical security applications</title> (2000)

The term 'computer generated hologram' (CGH) describes a diffractive structure strictly calculated and recorded to diffract light in a desired way. The CGH surface profile is a result of the wavefront calculation rather than of interference. CGHs are able to form 2D and 3D images. Optically, variable devices (OVDs) composed of diffractive gratings are often used in security applications. There are various types of optically and digitally recorded gratings in security applications. Grating based OVDs are used to record bright 2D images with limited range of cinematic effects. These effects result form various orientations or densities of recorded gratings. It is difficult to record high quality OVDs of 3D objects using gratings. Stereo grams and analogue rainbow holograms offer 3D imaging, but they are darker and have lower resolution than grating OVDs. CGH based OVDs contains unlimited range of cinematic effects and high quality 3D images. Images recorded using CGHs are usually more noisy than grating based OVDs, because of numerical inaccuracies in CGH calculation and mastering. CGH based OVDs enable smooth integration of hidden and machine- readable features within an OVD design.
Very low‐dose prednisolone in early rheumatoid arthritis retards radiographic progression over two years: A multicenter, double‐blind, placebo‐controlled trial (2005)

The very low daily dose of 5 mg prednisolone given over 2 years in combination with background DMARD therapy substantially decreased radiographic progression in early RA at low risk.
TCR Repertoire as a Novel Indicator for Immune Monitoring and Prognosis Assessment of Patients With Cervical Cancer (2018)

There is increasing evidence that deep sequencing-based T cell repertoire can sever as a biomarker of immune response in cancer patients; however, the characteristics of T cell repertoire including diversity and similarity, as well as its prognostic significance in patients with cervical cancer (CC) remain unknown. In this study, we applied a high throughput T cell receptor (TCR) sequencing method to characterize the T cell repertoires of peripheral blood samples from 25 CC patients, 30 cervical intraepithelial neoplasia (CIN) patients and 20 healthy women for understanding the immune alterations during the cervix carcinogenesis. In addition, we also explored the signatures of TCR repertoires in the cervical tumor tissues and paired sentinel lymph nodes from 16 CC patients and their potential value in predicting the prognosis of patients. Our results revealed that the diversity of circulating TCR repertoire gradually decreased during the cervix carcinogenesis and progression, but the circulating TCR repertoires in CC patients were more similar to CIN patients than healthy women. Interestingly, several clonotypes uniquely detected in CC patients tended to share similar CDR3 motifs, which differed from those observed in CIN patients. In addition, the TCR repertoire diversity in sentinel lymphatic nodes from CC patients was higher than in tumor tissues. More importantly, less clonotypes in TCR repertoire of sentinel lymphatic node was associated with the poor prognosis of the patients. Overall, our findings suggested that TCR repertoire might be a potential indicator of immune monitoring and a biomarker for predicting the prognosis of CC patients. Although functional studies of T cell populations are clearly required, this study have expanded our understanding of T cell immunity during the development of CC and provided an experimental basis for further studies on its pathogenesis and immunotherapy.
Preclinical activity of a novel CRM1 inhibitor in acute myeloid leukemia (2012)

Chromosome maintenance protein 1 (CRM1) is a nuclear export receptor involved in the active transport of tumor suppressors (e.g., p53 and nucleophosmin) whose function is altered in cancer because of increased expression and overactive transport. Blocking CRM1-mediated nuclear export of such proteins is a novel therapeutic strategy to restore tumor suppressor function. Orally bioavailable selective inhibitors of nuclear export (SINE) that irreversibly bind to CRM1 and block the function of this protein have been recently developed. Here we investigated the antileukemic activity of KPT-SINE (KPT-185 and KPT-276) in vitro and in vivo in acute myeloid leukemia (AML). KPT-185 displayed potent antiproliferative properties at submicromolar concentrations (IC50 values; 100-500 nM), induced apoptosis (average 5-fold increase), cell-cycle arrest, and myeloid differentiation in AML cell lines and patient blasts. A strong down-regulation of the oncogene FLT3 after KPT treatment in both FLT3-ITD and wild-type cell lines was observed. Finally, using the FLT3-ITD-positive MV4-11 xenograft murine model, we show that treatment of mice with oral KPT-276 (analog of KPT-185 for in vivo studies) significantly prolongs survival of leukemic mice (P < .01). In summary, KPT-SINE are highly potent in vitro and in vivo in AML. The preclinical results reported here support clinical trials of KPT-SINE in AML.
Impact of Oxidation Catalysts on Exhaust NO<sub>2</sub>/NO<sub>x</sub> Ratio from Lean-Burn Natural Gas Engines (2010)

Oxides of nitrogen (NOx) emitted from internal combustion engines are composed primarily of nitric oxide (NO) and nitrogen dioxide (NO2). Exhaust from most combustion sources contains NOx composed primarily of NO. There are two important scenarios specific to lean-burn natural gas engines in which the NO2/NOx ratio can be significant: (1) when the engine is operated at ultralean conditions and (2) when an oxidation catalyst is used. Large NO2/NOx ratios may result in additional uncertainty in NOx emissions measurements because the most common technique (chemiluminescence) was developed for low NO2/NOx ratios. In this work, scenarios are explored in which the NO2/NOx ratio can be large. Additionally, three NOx measurement approaches are compared for exhaust with various NO2/NOx ratios. The three measurement approaches are chemiluminescence, chemical cell, and Fourier-transform infrared spectroscopy. A portable analyzer with chemical cell technology was found to be the most accurate for measuring exhaust NOx with large NO2/NOx ratios.
Molecular addresses of tumors: selection by in vivo phage display (2006)

In vivo phage display has been used extensively to screen for novel targets of tumor therapy. Phage display peptide libraries can express random peptides or protein fragments and the aim of phage display is to identify peptide molecules that bind stably to a given target. Angiogenesis is essential to tumor development. Both blood and lymphatic vessels of tumors are different from those of normal tissues. Phage display has been used to analyze the structure and molecular diversity of tumor vasculature and to select tumor-specific antigens which have revealed stage- and type-specific markers of tumor blood vessels. Furthermore, peptides identified by in vivo phage display also work as vehicles to transport cargo therapeutic reagents to tumors. These peptides and their corresponding cellular proteins and ligands may provide molecular tools to selectively target the addresses of tumors and their pathological blood vessels and might increase the efficacy of therapy while decreasing side effects.
The Mixed-Lineage Kinase Inhibitor URMC-099 Protects Hippocampal Synapses in Experimental Autoimmune Encephalomyelitis (2018)

Treatments to stop gray matter degeneration are needed to prevent progressive disability in multiple sclerosis (MS). We tested whether inhibiting mixed-lineage kinases (MLKs), which can drive inflammatory microglial activation and neuronal degeneration, could protect hippocampal synapses in C57BL/6 mice with experimental autoimmune encephalomyelitis (EAE), a disease model that recapitulates the excitatory synaptic injury that occurs widely within the gray matter in MS. URMC-099, a broad spectrum MLK inhibitor with additional activity against leucine-rich repeat kinase 2 (LRRK2) and other kinases, prevented loss of PSD95-positive postsynaptic structures, shifted activated microglia toward a less inflammatory phenotype, and reversed deficits in hippocampal-dependent contextual fear conditioning in EAE mice when administered after the onset of motor symptoms. A narrow spectrum inhibitor designed to be highly selective for MLK3 failed to protect synapses in EAE hippocampi, and could not rescue cultured neurons from trophic deprivation in an <i>in vitro</i> model of MLK-driven neuronal degeneration. These results suggest that URMC-099 may have potential as a neuroprotective treatment in MS and demonstrate that a broad spectrum of inhibition against a combination of MLK and other kinases is more effective in neuroinflammatory disease than selectively targeting a single kinase.
A Novel Flexible Thermoelectric Sensor for Intravascular Flow Assessment (2013)

To accurately assess the severity of coronary artery disease, intracoronary pressure and flow measurements are required. In this paper, a novel flexible flow sensor, intended to be mounted on a medical guidewire, is tested experimentally. The device consists of a heating element and two thermopiles embedded in polyimide to measure flow-dependent heat transfer. The main aim of this paper is to determine whether constant temperature (CT) operation of the heater is feasible and is an improvement of the sensor response in unsteady flow compared with constant power (CP) operation. Thus, the flexible devices are glued to a surface and subjected to steady and unsteady water flows. From the relation between the sensor output and the applied shear rate it is shown that CT operation is superior over CP operation. Subsequently, a quasi-steady relation is identified and tested for coronary shear rate dynamics and found to be very accurate when the heater is operated at a CT difference of only 5 K, with an average error of 5%.