gebhart/patrepeval huggingface dataset:
Dengue Fever in US Military Personnel in Haiti (1997)

Active surveillance, with clinical and laboratory evaluation directed by an epidemiologic team, led to the timely recognition of an outbreak of febrile illness among US troops in Haiti. Viral isolation and serological studies were essential in confirming DF. During the surveillance period, DF accounted for at least 30% of the febrile illnesses among hospitalized US troops. Dengue fever is a significant threat to military personnel and civilian travelers in Haiti and has the potential for introduction to and transmission in the United States.
Generation of Human Alloantigen-Specific Regulatory T Cells under Good Manufacturing Practice-Compliant Conditions for Cell Therapy (2014)

Natural regulatory T cells (Tregs) may have a great therapeutic potential to induce tolerance in allogeneic cells and organ transplantations. In mice, we showed that alloantigen-specific Tregs (spe-Tregs) were more efficient than polyclonal Tregs (poly-Tregs) in controlling graft-versus-host disease (GVHD). Here we describe a clinical-grade compliant method for generating human spe-Tregs. Tregs were enriched from leukapheresis products with anti-CD25 immunomagnetic beads, primed twice by allogeneic mature monocyte-derived dendritic cells (mDCs), and cultured during 3 weeks in medium containing interleukin 2 (IL-2), IL-15, and rapamycin. After 3 weeks of culture, final cell products were expanded 8.3-fold from the initial CD25(+) purifications. Immunophenotypic analyses of final cells indicate that they were composed of 88 ± 2.6% of CD4(+) T cells, all expressing Treg-specific markers (FOXP3, Helios, GARP, LAP, and CD152). Spe-Tregs were highly suppressive in vitro and also in vivo using a xeno-GVHD model established in immunodeficient mice. The specificity of their suppressive activity was demonstrated on their ability to significantly suppress the proliferation of autologous effector T cells stimulated by the same mDCs compared to third-party mDCs. Our data provide evidence that functional alloantigen Tregs can be generated under clinical-grade compliant conditions. Taking into account that 130 × 10(6) CD25(+) cells can be obtained at large scale from standard leukapheresis, our cell process may give rise to a theoretical final number of 1 × 10(9) spe-Tregs. Thus, using our strategy, we can propose to prepare spe-Tregs for clinical trials designed to control HLA-mismatched GVHD or organ transplantation rejection.
Fabrication, characterization and modelling of electrostatic micro-generators (2009)

This paper presents an electrostatic energy-harvesting device for electrical energy extraction from vibrations. We successfully fabricated prototypes of completely packaged micro-generators with a chip size of 5 mm by 6 mm. This was achieved using a modified SOI technology developed for inertial sensors at HSG-IMIT. Micro-generators produce a maximum rms power of 3.5 µW when they are excited at their resonance frequency with an input excitation of 13 g. During a long-term experiment over a period of 2 h, the electrostatic energy harvester generated a total net energy of 13.38 mJ corresponding to an average power of 1.58 µW. The effect of mechanical stoppers and the bias voltage on the generated power is also evaluated. In order to get a more profound understanding of the dynamic behaviour of the micro-generator, we have developed a signal-flow model for numerical simulation of the electrostatic transducer on system level. This model includes a mechanical and an electrical domain which are coupled by electrostatic forces. The limited displacement of the proof mass is also considered using an elastic stopper model. We show that the numerical model is capable of providing good predictions of the device behaviour.
<i>APOBEC3B</i>expression in breast cancer reflects cellular proliferation, while a deletion polymorphism is associated with immune activation (2015)

Genomic sequencing studies of breast and other cancers have identified patterns of mutations that have been attributed to the endogenous mutator activity of APOBEC3B (A3B), a member of the AID/APOBEC family of cytidine deaminases. A3B gene expression is increased in many cancers, but its upstream drivers remain undefined. Furthermore, there exists a common germ-line deletion polymorphism (A3B(del)), which has been associated with a paradoxical increase in breast cancer risk. To examine causes and consequences of A3B expression and its constitutive absence in breast cancer, we analyzed two large clinically annotated genomic datasets [The Cancer Genome Atlas (TCGA) and the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC)]. We confirmed that A3B expression is associated with aggressive clinicopathologic characteristics and adverse outcomes and show that A3B expression is highly correlated with proliferative features (mitosis and cell cycle-related gene expression) in breast and 15 of 16 other solid tumor types. However, breast cancers arising in homozygous A3B(del) individuals with A3B absent did not differ in these features, indicating that A3B expression is a reflection rather than a direct cause of increased proliferation. Using gene set enrichment analysis (GSEA), we detected a pattern of immune activation in A3B(del) breast cancers, which seems to be related to hypermutation arising in A3B(del) carriers. Together, these results provide an explanation for A3B overexpression and its prognostic effect, giving context to additional study of this mutator as a cancer biomarker or putative drug target. In addition, although immune features of A3B(del) require additional study, these findings nominate the A3B(del) polymorphism as a potential predictor for cancer immunotherapy.
In vivo interactions of the NahR transcriptional activator with its target sequences. Inducer-mediated changes resulting in transcription activation (1991)

The nahR gene from the NAH7 naphthalene degradation plasmid encodes a LysR-type transcriptional activator of the nah and sal promoters (Pnah and Psal, respectively) that responds to the inducer salicylate. In vivo methylation protection experiments with dimethyl sulfate showed that in the absence of inducer, NahR interacts in a similar manner with its target sites at Psal and Pnah. Both target sites also have very similar sequences comprised of a 4-base pair interrupted dyad containing two symmetrical guanines (-73 and -64 of Pnah; -71 and -62 of Psal), each located in adjacent major grooves on the same helical face, and both strongly protected by NahR. When inducer was present, several additional guanines of Pnah (-35, -45, and -58) and Psal (-42 and -40) became protected from methylation, while a guanine at -52 of Pnah became markedly enhanced for methylation, indicating that inducer and NahR-dependent interactions with these downstream sites of each promoter are quite different. Deletion of Psal sequences downstream of -30 did not affect its methylation patterns suggesting that NahR alone is responsible for the altered reactivities of these nucleotides. Similar in vivo methylation analyses with inducer-insensitive or inducer-independent NahR mutants also suggested that all alterations in methylation sensitivity are directly caused by NahR. It is more probable that the salicylate-induced reactivity changes result from direct NahR-guanine contacts which are required for, but not sufficient for transcription activation; however, they could also result from NahR-induced DNA contortions caused by upstream protein-DNA contacts.
The GGS spacecraft, solar arrays for space physics (1996)

Two Global Geospace Science (GGS) spacecraft, WIND and POLAR, support highly sensitive instruments for measurements in the plasma source and plasma storage regions of geospace. The increased sensitivity of the electric and magnetic field instruments on the GGS missions required NASA Goddard Space Flight Center and Lockheed Martin Astro Space (LMAS) to explore new and innovative methods of providing an electrostatically clean, equipotential surface that would minimize interference with the surrounding plasma environment. This paper summarizes the qualification program, test results, flight build and on orbit performance of the GGS solar arrays.
The role of acylation in exopolysaccharides including those for food use (1992)

Abstract Microbial exopolysaccharides with potential for food usage, frequently carry acyl groups which may profoundly affect their interactive properties. Thus xanthan with or without acylation shows marked differences in synergis‐tic gelling with plant gluco‐ and galacto‐mannans, although the poly‐saccharides with different acylation patterns show similar viscosity. Similarly gellan from Auromonas elodea is of greater potential value after deacetylation, when it provides a valuable gelling agent, than it is as a viscosifier in the natural acylated form. The Enterobacter XM6 polymer only forms gels when it, too, has been chemically deacetylated. Both these polysaccharides form gels due to the enhanced interaction with cations following deacylation. Recent work in our laboratory suggests that deacetylation of certain bacterial alginates also significantly increases ion binding.
AF10 is split by MLL and HEAB, a human homolog to a putative Caenorhabditis elegans ATP/GTP-binding protein in an invins(10;11)(p12;q23q12). (1996)

Invins(10;11)(p12;q23q12) is one of the rare but recurring chromosome rearrangements seen in acute monoblastic leukemia. We cloned the proximal 10p breakpoint from one patient and showed that the MLL gene at 11q23 was fused to the 3' portion of AF10 at 10p12. In addition, we cloned the telomeric 10p junction and we found that the 5' portion of AF10 was juxtaposed to a previously unidentified gene at 11q12, which we call HEAB (a human homolog to a hypothetical Caenorhabditis elegans ATP/GTP-binding protein). These results indicate that the AF10 gene is split into a 5' AF10 and a 3' AF10 portion by the 11q23q12 chromosome segment and that both breakpoint junctions result in fusion transcripts of 5' AF10/HEAB and MLL/3' AF10. Only the MLL/3' AF10 fusion mRNA results in an in-frame fusion. Northern blot analysis of HEAB expression shows that a 2.0-kb major transcript is expressed ubiquitously in human tissues and is especially abundant in testis and skeletal muscle, whereas a 3.2-kb minor transcript is noted with the highest level of expression in thymus and peripheral blood leukocytes. The HEAB gene encodes a 425-amino acid protein that is rich in valine and leucine. HEAB protein shows high homology in its entire amino acid sequence to a putative C elegans protein and contains an adenosine triphosphate (ATP)/guanosine triphosphate (GTP)-binding motif that has homology to the ATP-binding transporter superfamily or to GTP-binding proteins. Our results could explain the high frequency of complex insertion and other rearrangement events that involve 10p12 and 11q12 and 11q23. The finding that different portions of a single gene are involved in fusions with two independent genes in the same leukemic cell is unique in the analysis of chromosome translocations.
Capacitive Analysis of Twisted Nematic Liquid Crystal Displays (1977)

Abstract Magnetocapacitive null (MCN) and capacitance versus voltage (C-V) measurements are valuable tools for analyzing electro-optical properties of twisted bematic LCDs. the C-V technique is particularly useful when resulrs are presented in the form of reduced capacitance CR(V) = [C(V) - C]/(C∥ - C1) = sin2α, where αe(V) is an effective molecular tilt. Thus, the effective voltage induced distortion of the LC is determined by monitoring CR-V.
MoodViews: Tracking and Searching Mood-Annotated Blog Posts (2007)

We demonstrate the next release of MoodViews, a set online tools mood analysis in blogs. Since its initial launch in mid-2005, MoodViews has provided a window into aggregate states of mind of masses of people. In addition to the tracking functionalities that MoodViews has offered so far, we demonstrate several types of mood-related search tools. These include searching for moods most closely associated with a given topic, and ranking blog posts not just by publication date or relevancy for a topic (as most blog search engines do), but also by mood (e.g., the “happiest” post on a given topic).
Identification of the heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1. (1989)

The heat-labile hemolysin of Actinobacillus pleuropneumoniae serotype 1 strain CM-5 was partially purified using ammonium sulfate precipitation and gel permeation chromatography. This partially purified material was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotted onto nylon filters. The filters were treated with convalescent pig serum and subsequently with CM-5 culture supernatant containing active hemolysin. A 104 kd peptide was identified as the hemolysin because it bound antibodies in convalescent pig serum which cross-linked active hemolysin. The same 104 kd protein when injected into a rabbit produced neutralizing antibodies to the CM-5 hemolysin in culture supernatant.
Assembled Structures and Chiroptical Properties of Amphiphilic Block Copolymers Synthesized by RAFT Polymerization of <i>N</i>‐Acryloyl‐<scp>L</scp>‐alanine (2008)

Abstract Amino acid‐based amphiphilic block copolymers involving poly( N ‐acryloyl‐ L ‐alanine), poly(A‐Ala‐OH), which exhibits a characteristic chiroptical property and pH‐dependent solubility, have been synthesized by reversible addition‐fragmentation chain transfer (RAFT) polymerization. The direct polymerization of A‐Ala‐OH without any protecting chemistry using the dithiocarbamate‐terminated polystyrene or poly( N ‐acryloyl‐ L ‐phenylalanine methyl ester) as a macrochain transfer agent produced well‐defined amphiphilic block copolymers. The self‐assembly behaviors and chiroptical properties of these amphiphilic block copolymers in selective solvents were investigated by dynamic light scattering, circular dichroism, and UV–Vis spectroscopic methods. magnified image
Electroless metalization of halloysite, a hollow cylindrical 1:1 aluminosilicate of submicron diameter (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectroless metalization of halloysite, a hollow cylindrical 1:1 aluminosilicate of submicron diameterSubhash Baral, Susan Brandow, and Bruce P. GaberCite this: Chem. Mater. 1993, 5, 9, 1227–1232Publication Date (Print):September 1, 1993Publication History Published online1 May 2002Published inissue 1 September 1993https://pubs.acs.org/doi/10.1021/cm00033a008https://doi.org/10.1021/cm00033a008research-articleACS PublicationsRequest reuse permissionsArticle Views367Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterization of detergent-solubilized sarcoplasmic reticulum calcium ATPase by high-performance liquid chromatography (1986)

Sarcoplasmic reticulum Ca2+-ATPase solubilized by the nonionic detergent octaethylene glycol monododecyl ether was studied by molecular sieve high-performance liquid chromatography (HPLC) and analytical ultracentrifugation. Significant irreversible aggregation of soluble Ca2+-ATPase occurred within a few hours in the presence of less than or equal to 50 microM Ca2+. The aggregates were inactive and were primarily held together by hydrophobic forces. In the absence of reducing agent, secondary formation of disulfide bonds occurred. The stability of the inactive dimer upon dilution permitted unambiguous assignment of its elution position and sedimentation coefficient. At high Ca2+ concentration (500 microM), monomeric Ca2+-ATPase was stable for several hours. Reversible self-association induced by variation in protein, detergent, and lipid concentrations was studied by large-zone HPLC. The association constant for dimerization of active Ca2+-ATPase was found to be 10(5)-10(6) M-1 depending on the detergent concentration. More detergent was bound to monomeric than to dimeric Ca2+-ATPase, even above the critical micellar concentration of the detergent. Binding of Ca2+ and vanadate as well as ATP-dependent phosphorylation was studied in monomeric and in reversibly associated dimeric preparations. In both forms, two high-affinity Ca2+ binding sites per phosphorylation site existed. The delipidated monomer purified by HPLC was able to form ADP-insensitive phosphoenzyme and to bind ATP and vanadate simultaneously. These results suggest that formation of Ca2+-ATPase oligomers in the membrane is governed by nonspecific forces (low affinity) and that each polypeptide chain constitutes a functional unit.
Cell Surface Glycan Alterations in Epithelial Mesenchymal Transition Process of Huh7 Hepatocellular Carcinoma Cell (2013)

The findings of this study systematically clarify the alterations of cell surface glycan in cancer EMT, and may provide novel insight for HCC metastasis.
Designing Fast-Dissolving Orodispersible Films of Amphotericin B for Oropharyngeal Candidiasis (2019)

Amphotericin B possesses high activity against <i>Candida</i> spp. with low risk of resistance. However, Amphotericin B's high molecular weight compared to other antifungal drugs, such as miconazole and clotrimazole, and poor water solubility hampers its efficacy at the physiological conditions of the oropharyngeal cavity (saliva pH, limited volume for dissolution) and thereby limits its clinical use in oropharyngeal candidiasis. We have prepared fast-dissolving orodispersible films with high loading (1% <i>w/w</i>) using solvent casting that enables amphotericin B to remain solubilised in saliva in equilibrium between the monomeric and dimeric states, and able to produce a local antifungal effect. Optimisation of the amphotericin B-loaded orodispersible films was achieved by quality by design studies combining dextran and/or maltodextrin as dextrose-derived-polymer film formers with cellulose-derived film formers (hydroxypropylmethyl/hydroxypropyl cellulose in a 1:4 weight ratio), sorbitol for taste masking, microcrystalline cellulose (Avicel 200) or microcrystalline cellulose-carboxymethylcellulose sodium (Avicel CL-611) for enhancing the mechanical strength of the film, and polyethylene glycol 400 and glycerol (1:1 <i>w/w</i>) as plasticizers. The optimised amphotericin B orodispersible films (containing 1% AmB, 25% dextran, 25% maltodextrin, 5% sorbitol, 10% Avicel 200, 10% polyethylene glycol 400, 10% glycerol, 3% hydroxypropylmethyl cellulose acetate succinate, 12% hydroxypropyl cellulose) possessed a fast disintegration time (60 ± 3 s), quick release in artificial saliva (>80% in 10 min), high burst strength (2190 mN mm) and high efficacy against several <i>Candida</i> spp. (<i>C. albicans</i>, <i>C. parapsilosis</i> and <i>C. krusei</i>) (>15 mm inhibition halo). Amphotericin B orodispersible films are stable for two weeks at room temperature (25 °C) and up to 1 year in the fridge. Although further toxicological and in vivo efficacy studies are required, this novel Amphotericin B orodispersible films is a promising, physicochemically stable formulation with potential wide application in clinical practice, especially for immunocompromised patients suffering from oropharyngeal candidiasis.
Wear-Resistance Assessment for Heavy-Duty Internal Combustion Engine Valvetrain Materials Application (2013)

The effects of pre-oxidation on the wear resistance of valve/valve-seat insert pairs for heavy-duty internal combustion engines were investigated using a reciprocating pin-on-plate testing procedure. This procedure, which is based on ASTM G133 [2010, “Standard Test Method for Linearly Reciprocating Ball-on-Flat Sliding Wear,” Annual Book of ASTM Standards, Vol. 03.02, ASTM International, West Conshohocken, PA], successfully ranked the wear resistance of four valve/valve-seat insert material pairs from 100°C to 550°C in 50°C increments. Wear of the pin and plate varied with temperature, but results suggest that promising improvements can be made in performance when one of the surfaces is pre-oxidized. Further research is needed to fully explore the friction and wear benefits of pre-oxidation treatments on the performance of valve and valve-seat alloys.
To Experience It Is to Know It (1973)

Here is some practical advice from a man who's been in the "hot seat of American education" long enough to want to talk about it.
An Approach to Evaluating Enterprise Financial Performance Based on the Control Inheritance of Family Businesses with Intuitionistic Fuzzy Information (2014)

We investigate the multiple attribute decision making problems for evaluating the enterprise financial performance based on the control inheritance of family businesses with intuitionistic fuzzy information. We utilize the intuitionistic fuzzy Bonferroni mean (IFBM) operator to aggregate the intuitionistic fuzzy information corresponding to each alternative and get the overall value of enterprise financial performance and then rank the enterprises and select the most desirable one(s) by using the overall value of enterprise financial performance. Finally, an illustrative example for evaluating the enterprise financial performance based on the control inheritance of family businesses is given to verify the developed approach and to demonstrate its practicality and effectiveness.
The Politics of Urban Design: The Center City Urban Renewal Project in Kansas City, Kansas (2003)

In contrast to other urban renewal projects that erased the presence of minority and working-class residents, the design of the Center City Plaza in downtown Kansas City, Kansas, was an attempt to provide a democratic space for a diverse citizenry. Initiated by local officials, the project was intended to alter the “image” of the downtown. Environmental planner Elpidio Rocha was hired to design a pedestrian mall that included abstract sculptural forms. In the context of deindustrialization and suburbanization, however, urban renewal did not halt downtown decline and local political interests dismantled the pedestrian mall, setting the stage for a new round of redevelopment.