gebhart/patrepeval huggingface dataset:
Characterization of the DNA-dependent ATPase and a DNA unwinding activity associated with the yeast DNA polymerase .alpha. complex (1993)

We have analyzed the ATPase and dATPase activities associated with the yeast DNA polymerase alpha complex. The ATPase/dATPase was primarily a single-stranded DNA-dependent ATPase. Analysis of the stimulatory effect of a large number of DNA substrates demonstrated that polynucleotides longer than 60 nucleotides (nts) had the maximal effect. The stimulation by oligonucleotides smaller than 60 nts, in general, decreased proportionally with decreased length of the oligomer. Poly- or oligopyrimidines were twice as stimulatory as the poly- or oligopurines of the same length. In addition to DNA, replication protein A (RP-A), a single-stranded DNA (ssDNA) binding protein, also stimulated the ATPase activity. Photo-cross-linking of the ATP binding component of the pol alpha complex to [alpha-32P]ATP at 0 degree C resulted in the exclusive labeling of a 90-kDa polypeptide. The labeling was inhibited by ATP and dATP but not by any other ribo- or deoxynucleotides, which suggest that the 90-kDa polypeptide is specific for ATP/dATP binding and possibly the active site for the ATPase/dATPase. We have also reported here a novel DNA unwinding activity associated with the multiprotein complex of DNA polymerase alpha. The complex was able to unwind M13mp19 ssDNA hybridized to an oligonucleotide (17-60 nucleotides long) with a protruding 3'-terminus. Regardless of the size of the duplex, the DNA unwinding was significantly stimulated by RP-A, while RP-A itself did not have any DNA unwinding activity. Consequently, it appeared that the DNA polymerase alpha complex possessed a putative RP-A-dependent helicase activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Low Anticoagulant Heparin Retains Anti-HIV Type 1 Activity <i>in Vitro</i> (1995)

Heparin is a potent inhibitor of HIV-1 replication, in addition to being a well-established inhibitor of blood coagulation. The major anticoagulant activity of heparin results from binding to the plasma protein antithrombin (AT). The high-affinity binding site for AT is a specific pentasaccharide sequence that is of low abundance and completely absent from the majority of heparin chains. We have examined the anti-HIV-1 activity of both conventional and low molecular weight heparins fractionated according to affinity for AT. The high- and low-affinity fractions, despite differing markedly in anticoagulant activity, are identical in their ability to bind to the envelope glycoprotein of HIV-1, and in their inhibitory effect on HIV-1 replication in vitro (EC50 1 and 8 micrograms/ml for conventional and low molecular weight fractions, respectively). Our study shows that the anti-HIV activity of heparin is independent of its antithrombin-mediated inhibition of coagulation proteases. Therefore, heparin preparations retaining full anti-HIV-1 activity in vitro but with greatly reduced anticoagulant activity may be readily produced for further clinical investigation in the prophylaxis and therapy of HIV infection.
Bimodal distribution of proteinase 3 (PR3) surface expression reflects a constitutive heterogeneity in the polymorphonuclear neutrophil pool (1995)

Proteinase 3, which is known as an intracellular serine protease of neutrophils, was detected at the surface of a subpopulation of freshly isolated PMN. The proportion of PR3-positive and -negative PMN, observed by flow cytometry with anti-PR3 mAbs or ANCA autoantibodies, varies among individuals but is extremely stable for each individual over prolonged time periods. After PMN degranulation by FMLP with cyt. B, membrane PR3 expression increases but the proportion of low and high PR3-expressing cells remains stable. The existence of a subset of PMN which spontaneously expresses PR3 and varies among individuals, may be relevant to the pathogenesis of anti-PR3 ANCA autoantibody-related vasculitis.
Market milk and related products (1938)

Market milk and related products , Market milk and related products , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
Electronic Kanji Dictionary Based on "Dasher" (2006)

A new interface is proposed for efficiently searching ideographic character sets such as the Japanese Kanji alphabet. The proposed interface is based on MacKay's Dasher, an interface originally designed to enable high-speed computer input for disabled persons. The Dasher interface allows a user to form words and sentences by "gliding" through a field of characters. This paper considers adapting the Dasher approach for gliding through a complex and unfamiliar character space. Information-theoretic algorithms are proposed to make the interface tolerant to variations among individuals' interpretations of Kanji characters, so that it is immediately usable by persons with no prior knowledge of Kanji. A software concept demonstration of the proposed dictionary is available, implementing some of the proposed features and algorithms
Study on improvement of efficiency of gas filter at the natural gas valve station (1998)

To eliminate foreign substance like metal rust and sand in natural gas pipeline, 250um strainer filter is used present in Korea Gas Corporation. But particles smaller than 250um passing the filter have bad effects to the valves and the measuring equipments. In order to eliminate small paticle in the pipeline, it is necessary to cut down the pore size of filter When we cut down the pore size of filter, the pressure difference between the front of filter and that of the rear part is increasing and disturb normal condition of gas supply. So it is very important to control the condition between the pore size of filter and the pressure difference. In this study, using head loss coefficient K, the estimation method of efficiency of gas filter according to the pore size and the pressure difference is presented.
Improving Performance of End-to-End ASR on Numeric Sequences (2019)

Recognizing written domain numeric utterances (e.g. I need $1.25.) can be challenging for ASR systems, particularly when numeric sequences are not seen during training. This out-of-vocabulary (OOV) issue is addressed in conventional ASR systems by training part of the model on spoken domain utterances (e.g. I need one dollar and twenty five cents.), for which numeric sequences are composed of in-vocabulary numbers, and then using an FST verbalizer to denormalize the result. Unfortunately, conventional ASR models are not suitable for the low memory setting of on-device speech recognition. E2E models such as RNN-T are attractive for on-device ASR, as they fold the AM, PM and LM of a conventional model into one neural network. However, in the on-device setting the large memory footprint of an FST denormer makes spoken domain training more difficult. In this paper, we investigate techniques to improve E2E model performance on numeric data. We find that using a text-to-speech system to generate additional numeric training data, as well as using a small-footprint neural network to perform spoken-to-written domain denorming, yields improvement in several numeric classes. In the case of the longest numeric sequences, we see reduction of WER by up to a factor of 8.
A comparison of cortical bone atrophy secondary to fixation with plates with large differences in bending stiffness (1976)

Two internal-fixation plates with large differences in bending stiffness were applied to pairs of intact femora of six adult mongrel dogs to study the osteoporosis induced by rigid fixation. After nine and twelve months of plate application a significant, increased amount of bone atrophy was seen on the rigidly plated side. Biomechanical measurements of specimens from various segments of both femoral diaphyses showed the bones to have similar mechanical properties (as tissue), but different structural properties (as organ). These findings suggest that the osteoporosis due to rigid plate fixation occurs by thinning of the cortex rather than by reduction of the mechanical properties of the osseous tissue.
A double-blind, randomized, placebo-controlled, active reference study of Lu AA21004 in patients with major depressive disorder (2011)

The efficacy, safety, and tolerability of Lu AA21004 vs. placebo using venlafaxine XR as active reference in patients with DSM-IV-TR major depressive disorder (MDD) were evaluated. Lu AA21004 is a novel antidepressant that is a 5-HT3 and 5-HT7 receptor antagonist, 5-HT1A receptor agonist, 5-HT1B receptor partial agonist and inhibitor of the 5-HT transporter in recombinant cell lines. In this 6-wk, multi-site study, 429 patients were randomly assigned (1:1:1:1) to 5 or 10 mg Lu AA21004, placebo or 225 mg venlafaxine XR. All patients had a baseline Montgomery-Åsberg Depression Rating Scale (MADRS) total score ≥ 30. The primary efficacy analysis was based on the MADRS total score adjusting for multiplicity using a hierarchical testing procedure starting with the highest dose vs. placebo. Lu AA21004 was statistically significantly superior to placebo (n=105) in mean change from baseline in MADRS total score at week 6 (p<0.0001, last observation carried forward), with a mean treatment difference vs. placebo of 5.9 (5 mg, n=108), and 5.7 (10 mg, n=100) points. Venlafaxine XR (n=112) was also significantly superior to placebo at week 6 (p<0.0001). In total, 30 patients withdrew due to adverse events (AEs)--placebo: four (4%); 5 mg Lu AA21004: three (3%); 10 mg Lu AA21004: seven (7%); and venlafaxine: 16 (14%). The most common AEs were nausea, headache, hyperhidrosis, and dry mouth. No clinically relevant changes over time were seen in the clinical laboratory results, vital signs, weight, or ECG parameters. In this study, treatment with 5 mg and 10 mg Lu AA21004 for 6 wk was efficacious and well tolerated in patients with MDD.
The Relationship Between Job Satisfaction and Performance: Evaluation and Utility for Management (1982)

Three major theoretical perspectives are postulated, viz, the hypotheses that: (1) satisfaction leads to performance;(2) the relationship is moderated by a number of variables; and (3) performance leads to satisfaction. Relevantresearch results are found to be equivocal at best, thereby implying that the relationship between satisfaction and performance is highly complex. It is suggested that, for more definitive interpretation to be facilitated, future studiesshould concentrate on attempting to discover potential moderating personal and situational variables. Important areas of concern for management arising out of the review include the psychological effects of job content,the matching of jobs to individual needs, the appropriateness of reward systems, and the use of performance appraisaland salary evaluation. All are seen as being pertinent to the quality of organisational functioning. It is therefore contended that a holistic contingency approach which takes into account the needs of workers, the characteristics of theirwork environment and the requirements of the organisation should be adopted.
Democracy and Danger on the Beach (2002)

Through an ethnographic study of a stretch of beach in Rio de Janeiro's Copacabana and Ipanema neighborhoods, the author argues that the public space of the city can act as a sort of public sphere where the politics of everyday class and race interaction can be part of larger scale politics, even in a very divided city like Rio de Janeiro. But Rio's beaches only confer a sort of marginal citizenship on their users. They are not the location of discursive democracy idealized by some social theorists, nor are they the egalitarian classless and color-blind spaces mythologized by the Brazilian elite. Rather, they are the site of an unequal, often confrontational politics of class whereby the legitimacy of the social order is challenged, renegotiated, and ultimately reproduced.
Segment-before-Detect: Vehicle Detection and Classification through Semantic Segmentation of Aerial Images (2017)

Like computer vision before, remote sensing has been radically changed by the introduction of deep learning and, more notably, Convolution Neural Networks. Land cover classification, object detection and scene understanding in aerial images rely more and more on deep networks to achieve new state-of-the-art results. Recent architectures such as Fully Convolutional Networks can even produce pixel level annotations for semantic mapping. In this work, we present a deep-learning based segment-before-detect method for segmentation and subsequent detection and classification of several varieties of wheeled vehicles in high resolution remote sensing images. This allows us to investigate object detection and classification on a complex dataset made up of visually similar classes, and to demonstrate the relevance of such a subclass modeling approach. Especially, we want to show that deep learning is also suitable for object-oriented analysis of Earth Observation data as effective object detection can be obtained as a byproduct of accurate semantic segmentation. First, we train a deep fully convolutional network on the ISPRS Potsdam and the NZAM/ONERA Christchurch datasets and show how the learnt semantic maps can be used to extract precise segmentation of vehicles. Then, we show that those maps are accurate enough to perform vehicle detection by simple connected component extraction. This allows us to study the repartition of vehicles in the city. Finally, we train a Convolutional Neural Network to perform vehicle classification on the VEDAI dataset, and transfer its knowledge to classify the individual vehicle instances that we detected.
Production of essential <scp>L</scp>‐branched‐chain amino acids in bioreactors containing artificial cells immobilized multienzyme systems and dextran–NAD<sup>+</sup> (1990)

We prepared artificial cells each containing leucine dehydrogenase (EC 1.4.1.9), urease (EC 3.5.1.5), soluble dextran-NAD(+), and one of the following coenzyme regenerating dehydrogenases: glucose dehydrogenase (EC 1.1.1.47); yeast alcohol dehydrogenase (EC 1.1.1.1); malate dehydrogenase (EC 1.1.1.37); or lactate dehydrogenase (EC 1.1.1.27). Artificial cells were packed in small columns. L-Leucine, L-valine, and L-isoleucine were continuously produced with simultaneous dextran-NADH regeneration. The maximum production ratios depended on the coenzyme regenerating systems used: 83-93% for D-glucose and glucose dehydrogenase system; 90% for ethanol and yeast alcohol dehydrogenase system; 45-55% for L-malate and malate dehydrogenase system; and 64-78% for L-lactate and lactate dehydrogenase system. Kinetic experiments were also carried out. The apparent K(m) values are as follows: 0.33 mM for alpha-ketoisocaproate (KIC); 0.51 mM for alpha-ketoisovalerate (KIV); 0.58 mM for DL-alpha-keto-beta-methyl-n-valerate (KMV); 3.52 mM for urea; 27.82 mM for D-glucose; 3.89 mM for ethanol; 3.02 mM for L-malate; and 16.67 mM for L-lactate. Kinetic analysis showed that KIC, KIV, and KMV were all competitive inhibitors in the reactions catalyzed by leucine dehydrogenase. Their inhibitor constants were the corresponding K(m) values.
Piezoelectric ceramic implants: <i>in vivo</i> results (1981)

The suitability of barium titanate (BaTiO3) ceramic for direct substitution of hard tissues was evaluated using both electrically stimulated (piezoelectric) and inactive (nonpolarized) test implants. Textured cylindrical specimens, half of them made piezoelectric by polarization in a high electric field, were implanted into the cortex of the midshaft region of the femora of dogs for various periods of time. Interfacial healing and bio-compatibility of the implant material were studied using mechanical, microradiographical, and histological techniques. Our results indicate that barium titanate ceramic shows a very high degree of biocompatibility as evidenced by the absence of inflammatory or foreign body reactions at the implant-tissue interface. Furthermore, the material and its surface porosity allowed a high degree of bone ingrowth as evidenced by microradiography and a high degree of interfacial tensile strength. No difference was found between the piezoelectric and the electrically neutral implant-tissue interfaces. Possible reasons for this are discussed. The excellent mechanical properties of barium titanate, its superior biocompatibility, and the ability of bone to form a strong mechanical interfacial bond with it, makes this material a new candidate for further tests for hard tissue replacement.
Physical access to computers: can your computer be trusted? (2002)

The proliferation of low cost computers has been a boon to productivity. Easier physical access, however, puts computer-based information at greater risk. The information at risk is not limited to unencrypted files stored in the exposed computer. Files encrypted using the exposed computer may be vulnerable. Any computer accessed through the exposed computer, or another computer on the same LAN, may be vulnerable. This paper examines some of the threats that can result from physical access. These include data scavenging, keystroke monitoring and network packet monitoring. Targeted information may be of value in its own right (primary information), or may provide the keys (secondary information) to protected information. Examples of secondary information targets include passwords or encryption keys.
Comparative biomechanical analysis of energy-storing prosthetic feet. (1992)

Gait analysis was performed on eight men who had unilateral traumatic below-knee amputation and on nine control subjects. Each subject was given two prostheses--the Seattle Foot and the Flex Foot--which differed only in the energy-storing foot component. Analysis of subjects consisted of clinical gait observation, forceplate analysis of the ground reaction force (GRF) while using each prosthesis during level walking at the natural cadence, and evaluation of subject preference between the two prosthetic feet. In the control subjects, there was no significant asymmetry in any averaged GRF patterns or parameters. In the subjects with amputations, the amputated limb had a weaker propulsion and the nonamputated limb had a stronger propulsion than controls. This was true for both prostheses. During ambulation with the Flex Foot, there was a pattern of larger late vertical forces but smaller late anteroposterior and mediolateral forces. This is consistent with a medial heel whip, and it was observed when the Flex Foot was used. Three months after the biomechanical studies, four subjects used the Flex Foot exclusively, two used the Seattle Foot exclusively, and two used both, ie, the Flex Foot for sports and the Seattle Foot for work. Application of these results to the choice of prosthetic components is discussed.
Involvement of Thrombin Anion-binding Exosites 1 and 2 in the Activation of Factor V and Factor VIII (1996)

The role of anion-binding exosites of thrombin in the activation of factor V and factor VIII was studied using thrombin Arg93 --> Ala, Arg97 --> Ala, and Arg101 --> Ala (thrombin RA), a recombinant exosite 2 defective mutant, and a synthetic N-acetylated dodecapeptide, Ac-Asn-Gly-Asp-Phe-Glu-Glu-Ile-Pro-Glu-Glu-Tyr-O-SO4Leu (hirugen), which competitively inhibits binding of macromolecules to exosite 1. The catalytic efficiency of the activation of factor VIII or of the first step of factor V activation by thrombin RA was approximately 10% that of wild-type thrombin. The overall rate of conversion to factor Va was not influenced by the mutation. In contrast to factor V, the slow activation of factor VIII by thrombin RA was associated with a decreased rate of cleavage at all three proteolytic sites (Arg372, Arg740, and Arg1689). Hirugen inhibited factor V and factor VIII activation. These results indicate that both anion-binding exosites of thrombin are involved in the recognition of factor V and factor VIII.
Integration of Chromatographic and Spectroscopic Techniques for the Characterization of Residual Oils (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntegration of Chromatographic and Spectroscopic Techniques for the Characterization of Residual OilsDouglas M. Jewell, Edgar W. Albaugh, Burl E. Davis, and Raffaele G. RubertoCite this: Ind. Eng. Chem. Fundamen. 1974, 13, 3, 278–282Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://pubs.acs.org/doi/10.1021/i160051a022https://doi.org/10.1021/i160051a022research-articleACS PublicationsRequest reuse permissionsArticle Views306Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Determination of iron, cobalt, copper, zinc, rubidium, molybdenum, and cesium in human serum by inductively coupled plasma mass spectrometry (1989)

A method was developed for the determination of seven trace elements (Fe, Co, Cu, Zn, Rb, Mo, and Cs) in human serum by inductively coupled plasma mass spectrometry (ICP-MS). Sample preparation was kept as limited as possible. As the only sample pretreatment serum samples were diluted with nitric acid and indium was added as an internal standard. The results for iron, cobalt, copper, and zinc were corrected for interferences from polyatomic ions by using a blank solution containing the same concentration of sodium, sulfur, chlorine, and calcium as human serum. For copper and zinc the corrections are small, whereas for iron and cobalt they are important. No interferences occur for the considered isotopes of rubidium, molybdenum, and cesium. In order to test the accuracy and precision of the analytical technique, a "second-generation biological reference material (human serum)" was analyzed. The results obtained by ICP-MS for the seven elements considered showed good agreement with the certified values.
Hydrogen-bonds structure in poly(2-hydroxyethyl methacrylate) studied by temperature-dependent infrared spectroscopy (2014)

Hydrogen-bonds structure in poly(2-hydroxyethyl methacrylate) (PHEMA) were investigated by means of temperature-dependent infrared (IR) spectroscopy. Spectral variations involved with the OH…OH and C=O…HO types of hydrogen-bonds were found around the glass transition temperature of 80°C. Hydrogen-bonds among the hydroxyl groups gradually dissociate with increasing temperature. In contrast, discontinuous variation in the carbonyl bands was observed around the glass transition temperature. An association of the C=O…HO type of hydrogen-bond with increasing temperature above the glass transition temperature was revealed. These were concluded from the present study that hydrogen-bonds among the hydroxyl groups in each side chain terminal suppress the main chain mobility in the polymer matrix below the glass transition temperature, while the dissociation of the OH…OH type of hydrogen-bonds induces the association of the C=O…HO type of hydrogen-bond. As a result, the mobility of the main chain is induced by the change in hydrogen-bonds structure at the glass transition temperature.