gebhart/patrepeval huggingface dataset:
Quantitative In Situ Detection of Phosphoproteins in Fixed Tissues Using Quantum Dot Technology (2009)

Detection and quantitation of phosphoproteins (PPs) in fixed tissues will become increasingly important as additional inhibitors of protein kinases enter clinical use and new disease entities are defined by molecular changes affecting PP levels. We characterize fixation conditions suitable for accurate PP quantitation that are achievable in a clinical laboratory and illustrate the utility of in situ quantitation of PPs by quantum dot (QD) nanocrystals in two models: (1) a therapeutic model demonstrating effects of a targeted therapeutic (quantitative reduction of phospho-GSK3beta) in xenografts treated with enzastaurin; and (2) a diagnostic model that identifies elevated levels of nuclear phospho-STAT5 in routine bone marrow biopsies from patients with acute myeloid leukemia based on the presence of the activating FLT3-ITD mutation. Finally, we document production of a well-characterized tissue microarray of widely available cell lines as a multilevel calibrator for validating numerous phosphoprotein assays. QD immunofluorescence is an ideal method for in situ quantitation of PPs in fixed samples, providing valuable cell type-specific and subcellular information about pathway activation in primary tissues.
Isolation of equine bone marrow‐derived mesenchymal stem cells: a comparison between three protocols (2010)

These data suggest that the Percoll gradient density separation protocol was the best in terms of MSC yield and self-renewal potential of the MSCs retrieved and that MSCs retrieved with the Ficoll protocol had the lowest self-renewal but only at passage 0. Then, the 3 protocols were equivalent. However, the Percoll protocol should be considered for equine MSC isolation to minimise culture time.
Fast Microwave Logic Circuits (1959)

In a carrier-type digital computer system, binary information can be represented by the presence or absence of an RF pulse in a given time interval. Using strip-line printed circuit techniques and point-contact diodes, passive AND and NOT gates were constructed which operate with RF pulses of less than 2 mμsec duration (i.e., an effective pulse repetition rate of 500 mc), at a carrier frequency of 3000 mc. The basic gates were combined to form half-adders. Unlike other carrier approaches, these circuits keep the information in RF form through all steps of the logic operations; i.e., both inputs and outputs of all elements are RF pulses.
Extraction with Supercritical Gases—A Foreword (1978)

A novel method of separation , extraction with supercritical gases, was the topic of a symposium held at Essen in June of this year. The present issue of Angewandte Chemie International Edition contains all the papers read at this symposium, and thus provides first‐hand information about the state of the art. An industrial plant for decaffeination of green coffee beans with supercritical CO 2 will shortly be coming on stream.
Increased production of superoxide anion by macrophages exposed in vitro to muramyl dipeptide or lipopolysaccharide. (1980)

After in vitro exposure to lipopolysaccharide (LPS) or muramyl dipeptide (MDP), cultured resident mouse peritoneal macrophages were primed to display enhanced generation of superoxide anion (O2-) in response to stimulation by phorbol myristate acetate (PMA) or opsonized zymosan. Priming with LPS (1 microgram/ml) produced a sevenfold enhancement of PMA-stimulated O2- generation; priming was detected within 30 min and persisted for at least 4 d. Exposure to MDP (1 muM) primed the macrophages to double their O2- release; the response was first observed after 4 h and persisted for at least 3 d. The priming response was not observed with stereoisomers of MDP, which are inactive as adjuvants. LPS and MDP appeared to work directly on the macrophages rather than indirectly by interacting with adherent lymphocytes: (a) Addition of nonadherent cell populations that contained lymphocytes had no effect on the response. (b) The response was normal with cells from nude mice, which lack mature T lymphocytes. (c) Macrophages from C3H/HeJ mice, whose B lymphocytes fail to respond to LPS, were weak in their response to priming LPS; the addition of normal (C3Heb/FeJ) nonadherent cells had no effect on this weak response. (d) The macrophage-like cell line J774.1 also showed enhanced O2--generating capacity after a 4-h exposure to LPS or MDP. The O2--generating capacity of macrophages primed with LPS in vitro was equivalent to that previously observed with cells elicited in vivo by injection of LPS or activated by infection with Bacille Calmette-Guérin. The data suggest that previous exposure to bacterial products could prime macrophages to respond with increased production of toxic oxygen metabolites on contact with invading microorganisms or tumor cells.
A 512-bit shift register using compact two-phase single flux quantum clock generators with large margins and low power (1993)

A 512-b shift register was built and tested up to 14.5 GHz. The shift register uses a two-phase clock which is generated by coupling a master control line over many asymmetrically biased two-junction SQUIDs. Compared with other shift registers with Josephson transmission lines to deliver clock, this new clock system provides short delay, low power dissipation, and large DC bias margins. The shift register uses about 3000 Nb/AlO/sub x//Nb Josephson junctions and consumes about 0.1 mW.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Ligand Binding Analysis for Human α5β1 Integrin:  Strategies for Designing New α5β1 Integrin Antagonists (2005)

We report a three-dimensional model of the alpha5beta1 integrin headgroup bound to the most potent and selective ligand (SJ749) known to date. The model was built using the comparative protein modeling method, and it is consistent with experimental data. From this study, we identified two potentially important regions in the alpha5beta1 receptor that are peculiar to this integrin and might be worth considering for drug targeting.
New Table of the Refractive Index of Pure Glycerol at 20°C (1934)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew Table of the Refractive Index of Pure Glycerol at 20°CL. F. HoytCite this: Ind. Eng. Chem. 1934, 26, 3, 329–332Publication Date (Print):March 1, 1934Publication History Published online1 May 2002Published inissue 1 March 1934https://pubs.acs.org/doi/10.1021/ie50291a023https://doi.org/10.1021/ie50291a023research-articleACS PublicationsRequest reuse permissionsArticle Views3319Altmetric-Citations131LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Double-pass self-spectral-calibration of a polarization state analyzer (2012)

We present a new method that allows efficient spectral calibration for a polarization state analyzer. The procedure does not require any additional polarization optical element other than the polarization state analyzer itself. It uses a double-pass technique that can be achieved up to a very good precision. The method is illustrated using real measurements done at several wavelengths with a rotating wave plate polarization state analyzer. Alignment of axis as well as true retardation at a specific wavelength are easily obtained by a standard function fitting.
Reducing the Influence of Anecdotal Reasoning on People’s Health Care Decisions: Is a Picture Worth a Thousand Statistics? (2005)

. Presenting statistical information using a pictograph can reduce the undue influence of anecdotal reasoning on treatment choices.
Filter-based NRD-guide duplexer with low loss and high isolation at 60 GHz (2004)

A filter-based duplexer has been developed for a wireless broadband asymmetric digital subscriber line by using NRD guide technology. The isolation between the transmitter port and the receiver port is more than 45 dB, and the insertion loss is less than 3 dB in the 60 GHz band.
Transient-State Analysis of Metabolic Fluxes in Crabtree-Positive and Crabtree-Negative Yeasts (1990)

In bakers' yeast, an immediate alcoholic fermentation begins when a glucose pulse is added to glucose-limited, aerobically grown cells. The mechanism of this short-term Crabtree effect was investigated via a comparative enzymic analysis of eight yeast species. It was established that the fermentation rate of the organisms upon transition from glucose limitation to glucose excess is positively correlated with the level of pyruvate decarboxylase (EC 4.1.1.1). In the Crabtree-negative yeasts, the pyruvate decarboxylase activity was low and did not increase when excess glucose was added. In contrast, in the Crabtree-positive yeasts, the activity of this enzyme was on the average sixfold higher and increased after exposure to glucose excess. In Crabtree-negative species, relatively high activities of acetaldehyde dehydrogenases (EC 1.2.1.4 and EC 1.2.1.5) and acetyl coenzyme A synthetase (EC 6.2.1.1), in addition to low pyruvate decarboxylase activities, were present. Thus, in these yeasts, acetaldehyde can be effectively oxidized via a bypass that circumvents the reduction of acetaldehyde to ethanol. Growth rates of most Crabtree-positive yeasts did not increase upon transition from glucose limitation to glucose excess. In contrast, the Crabtree-negative yeasts exhibited enhanced rates of biomass production which in most cases could be ascribed to the intracellular accumulation of reserve carbohydrates. Generally, the glucose consumption rate after a glucose pulse was higher in the Crabtree-positive yeasts than in the Crabtree-negative yeasts. However, the respiratory capacities of steady-state cultures of Crabtree-positive yeasts were not significantly different from those of Crabtree-negative yeasts. Thus, a limited respiratory capacity is not the primary cause of the Crabtree effect in yeasts. Instead, the difference between Crabtree-positive and Crabtree-negative yeasts is attributed to differences in the kinetics of glucose uptake, synthesis of reserve carbohydrates, and pyruvate metabolism.
A DVB/IP streaming testbed for hybrid digital media content synchronization (2012)

In the HbbTV specification, high precision hybrid inter-media synchronization is still an uncovered topic. In this paper we propose a new synchronization mechanism, which provides frame accurate synchronization for media from hybrid sources, like DVB and IP. Our solution is flexible in its application, as it can handle all kinds of audio/video content, and it is easy to implement in terms of solution complexity.
Twin boundaries and critical current densities of YBa<sub>2</sub>Cu<sub>3</sub>O<sub>7</sub>thick films fabricated by the BaF<sub>2</sub>process (2003)

2 µm thick epitaxial YBa2Cu3O7, YBCO, films on SrTiO3 substrates were prepared at the reaction temperatures of 725, 750 and 800 °C by the BaF2 process. The dc critical current densities of these films were found to vary by a factor of 2 at 77 K in perpendicular dc magnetic fields measured up to 1.5 T. The twin boundary spacing was essentially unchanged among these films while the size of Y2O3 precipitates in the films varied by a factor of ∼5. Also, the measured ratios of the lattice parameters, a/b, of the films were surprisingly larger than those found in standard fully oxygenated YBCO. This unusual property of the films appeared to be caused by the presence of the Y2O3 precipitates which provided a strain constraint in limiting the full tetragonal-to-orthorhombic phase transformation due to the oxygenation. Differences in the degree of the constraint possibly led to the observed difference in Jc among these films.
Male reproductive health: cause for concern? (2000)

A substantial body of evidence has accumulated in recent years that human semen quality may be deteriorating. This has been associated with evidence of other changes in male reproductive health, including increases in congenital malformations and testicular cancer in humans, and similar problems in wildlife. Unfortunately, the evidence remains inconclusive. It has been suggested that these changes may be due to environmental xeno-oestrogens acting during development. Although there is now a large quantity of data indicating that this is a plausible hypothesis, evidence of causality, rather than association, remains to be provided. The potential importance of these changes for human health is considerable and urgent research is required to clarify the situation.
The danger of turning a drama into an emergency (2002)

What does the Nursing and Midwifery Council define as an emergency (letters July 17)? Any injury may be perceived to be an emergency at the time.
RADIOIMMUNOASSAY OF LUTEINIZING HORMONE RELEASING FACTOR (1973)

The decapeptide luteinizing hormone releasing factor (LH-RF) has recently been isolated, sequenced and synthesized (Schally, Arimura, Kastin, Matsuo, Baba, Redding, Nair, Debeljuk & White, 1971). This has made possible the development of radioimmunoassays for this factor enabling it to be measured in biological fluids in vivo and in vitro. Two milligrammes of the synthetic decapeptide (Hoechst) were conjugated to 2 mg bovine serum albumin (BSA) using 75 mg 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide in 0·25 ml water (Goodfriend, Levine & Fasman, 1964). After dialysis overnight, 1 mg of the conjugate in 2 ml water, emulsified in Freund's complete adjuvant, was injected into 20 intradermal sites in a white New Zealand rabbit. A blood sample was obtained 8 weeks after this primary immunization and the assay developed using this antiserum. LH-RF (0·1–1 μg) was iodinated with 125I (0·5–1 mCi) by the chloramine-T technique (5 μg of chloramine-T), specific activities between 100 and 500 μCi/μg being obtained.
Advances In Organic Electro-Optic Devices (1988)

Organic and polymeric materials have many useful features for thin-film electro-optic devices. These include low dielectric constants, moderate-to-large electro-optic coefficients, and low optical loss. This paper presents a review of the useful features of organic materials for device applications, summarizes the current optical response levels of some organic materials, and describes the performance of several device prototypes fabricated from poled organic films.
P2X7 and NRAMP1/SLC11 A1 gene polymorphisms in Mexican mestizo patients with pulmonary tuberculosis (2007)

Tuberculosis remains one of the most important infectious diseases worldwide. Several studies have suggested that genetic factors may affect susceptibility to tuberculosis, but the specific genes involved have not yet been fully characterized. NRAMP1/SLC11 A1 and P2X(7) genes have been linked to increased risk for tuberculosis in some African and Asiatic populations. To explore the potential role of these genes in the susceptibility to pulmonary tuberculosis in a Mexican mestizo population, we evaluated the association of D543N and 3'-UTR polymorphisms in NRAMP1/SLC11 A1 and - 762 and A1513C polymorphisms in P2X(7) genes with the risk for tuberculosis. Polymerase chain reaction (PCR) amplification of genomic DNA followed by restriction fragment length polymorphism analysis, and allelic-specific PCR was employed. We found no significant differences in allelic frequency in NRAMP1/SLC11 A1 gene polymorphisms in 94 patients with tuberculosis compared to 100 healthy contacts. Similarly, no significant association of the P2X(7)-762 gene polymorphism with tuberculosis was detected. In contrast, the P2X(7) A1513C polymorphism was associated significantly with tuberculosis (P = 0.02, odds ratio = 5.28, 95% CI, 0.99-37.69), an association that had not been reported previously. However, when the function of P2X(7) was assessed by an L-selectin loss assay, we did not find significant differences in patients compared to healthy contacts or between PPD(+) and PPD(-) control individuals. This study further supports the complex role of P2X(7) gene in host regulation of Mycobacterium tuberculosis infection, and demonstrates that different associations of gene polymorphisms and tuberculosis are found in distinct racial populations.
Self-Defense, The Law of Armed Conflict and Port Security (2009)

Symposium colleagues have analyzed the law of the sea (LOS) and U.S. law as they relate to ports security.'This article surveys other situations, i.e., the intersection of port security issues with law of selfdefense and actions under the U.N. Charter, and the law of armed conflict (LOAC) as it affects the oceans, historically styled the law of naval warfare and neutrality.Jus cogens principles, i.e., fundamental norms transcending the usual sources of international law, may apply in these situations.Sometimes the results are the same as those under the law of the sea or U.S. law; in others they are, or can be, radically different.Part I analyzes the law of self-defense and U.N. Charter law that may affect ports and ports security.Part II briefly discussesjus cogens, a relatively new concept of superior norms trumping traditional sources of international law.Part III surveys armed conflict situations that may affect port security issues and the conduct of military operations.Part IV discusses the legitimacy under international law of certain state actions relating to ports.
The separation of the intramolecular isotope effect for the cytochrome P-450 catalyzed hydroxylation of n-octane into its primary and secondary components (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe separation of the intramolecular isotope effect for the cytochrome P-450 catalyzed hydroxylation of n-octane into its primary and secondary componentsJefferey P. Jones and William F. TragerCite this: J. Am. Chem. Soc. 1987, 109, 7, 2171–2173Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/ja00241a040https://doi.org/10.1021/ja00241a040research-articleACS PublicationsRequest reuse permissionsArticle Views139Altmetric-Citations27LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ferrite particles: a superparamagnetic MR contrast agent for the reticuloendothelial system. (1987)

The potential of superparamagnetic ferrite particles as a contrast agent for magnetic resonance (MR) imaging was studied by in vitro MR spectroscopy and in vivo MR imaging in laboratory animals. After aqueous preparations of ferrite particles were administered intravenously, MR spectroscopy showed greatly decreased T2 relaxation times of liver and spleen, with only minimally altered T1, and no changes in lung, kidney, or muscle. Effects occurred within 30 minutes of injection and persisted for more than 6 months. MR imaging with pulse sequences that provide T2-dependent contrast demonstrated that ferrite produced profound signal loss from liver, spleen, and bone marrow. Sequestration of ferrite particles in hepatic reticuloendothelial cells was confirmed by means of light and electron microscopy. Because ferrite has a potent effect on MR signal and exhibits tissue-specific localization, it warrants further study as a contrast agent for MR imaging of the reticuloendothelial system (i.e., liver, spleen, and bone marrow).
Reversal of the inhibitory action of abscisic acid by benzyladenine in excised watermelon cotyledons (1981)

Cotyledons of watermelon ( Citrullus vulgaris Schrad. cv. Fairfax) were excised from the embryo after 24 h of imbibition and cultured for several days on filter paper with water or abscisic acid (ABA) solution. In some experiments the cotyledons were pretreated with benzyladenine (BA) for times ranging from 5 min to 2 h before transfer to ABA. A treatment with 10 −5 M ABA blocked all developmental parameters examined (growth and increase in appropriate markers for glyoxysome, peroxisome and plastid development). This blocking can be prevented by an initial treatment with 10 −4 M BA for 2 h. This pretreatment with BA overrides the action of ABA: the final developmental responses are not just restored to the level of the water control, but they are almost as high as those obtained by treating the cotyledons with BA only. If BA is administered for three days together with ABA the reversal of inhibition is much less efficient.
A novel uplink channel estimation in OFDM-CDMA systems (2004)

Orthogonal frequency division multiplexing-code division multiplex access (OFDM-CDMA) is one of the best candidates for the future broadband wireless multimedia communication systems. This paper proposes a novel channel estimation algorithm for OFDM-CDMA systems on the uplink. With reasonable constraint and well-designed preambles for each user, the DFT-based uplink channel estimation algorithm can achieve good estimation accuracy without sacrificing much system overhead. Computer simulation demonstrates effectiveness of this method and conclusion is followed.
Handbook of Encapsulation and Controlled Release (2015)

The field of encapsulation, especially microencapsulation, is a rapidly growing area of research and product development. The Handbook of Encapsulation and Controlled Release covers the entire field, presenting the fundamental processes involved and exploring how to use those processes for different applications in industry. Written at a level comp
Composition dependence of Au/In<i>x</i>Al1−<i>x</i>As Schottky barrier heights (1986)

The surface barrier heights φbn and room-temperature band gaps Eg of Si-doped InxAl1−xAs layers grown by molecular beam epitaxy on n-type (100) oriented InP substrates have been determined as a function of composition with capacitance versus voltage, internal photoemission, photoluminescence, and double-crystal x-ray rocking curve measurements for 0.45&lt;x&lt;0.55. The results indicate that Eg and φbn are linear functions of x; they also suggest that φbn (0.78)=0 and, for x&gt;0.78, n-type surfaces might be accumulated and p-type surfaces are likely to be inverted.