gebhart/patrepeval huggingface dataset:
Evaluation of peroxyoxalate chemiluminescence for determination of enzyme generated peroxide (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvaluation of peroxyoxalate chemiluminescence for determination of enzyme generated peroxideDavid C. Williams, Glenn F. Huff, and W. Rudolf. SeitzCite this: Anal. Chem. 1976, 48, 7, 1003–1006Publication Date (Print):June 1, 1976Publication History Published online1 May 2002Published inissue 1 June 1976https://pubs.acs.org/doi/10.1021/ac60371a025https://doi.org/10.1021/ac60371a025research-articleACS PublicationsRequest reuse permissionsArticle Views234Altmetric-Citations80LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A location-based incentive mechanism for participatory sensing systems with budget constraints (2012)

Participatory sensing (PS) systems rely on the willingness of mobile users to participate in the collection and reporting of data using a variety of sensors either embedded or integrated in their cellular phones. However, this new data collection paradigm has not been very successful yet mainly because of the lack of incentives for participation. Although several incentive schemes have been proposed to encourage user participation, none has used location information and imposed budget and coverage constraints, which will make the scheme more realistic and efficient. We propose a recurrent reverse auction incentive mechanism with a greedy algorithm that selects a representative subset of the users according to their location given a fixed budget. Compared to existing mechanisms, our incentive scheme improves the area covered by more than 60 percent acquiring a more representative set of samples after every round while maintaining the same number of active users in the system and spending the same budget.
Technology for Controlling Eccentric Wear of Sucker Rods and Tubing in Highly Deviated Directional Well (2011)

Installing centralizer is a practicable measure for the problem of controlling eccentric wear of sucker rods in highly directional well. The location and quantity of centralizer is whether suited the eccentric wear of sucker rods and tubing in practical situation, and there is still not a measure to simulate the phenomenon after installing centralizer. With respect to this problem, this paper gives an introduction to a measure of calculation and three dimensional display.The main features to this measure are:utilize the logging data from measurement to simulate wellbore space trajectory, calculate the loads that make sucker rods bent in three dimensional space ( the location of eccentric wear);calculating the location of centralizer must be classified three situation: under pressure, excessive log-leg severity, considering the deflection;calculation analysis of force after installing centralizer on tubing and sucker rods;completed solution can be given by integrating the above situation and considering the engineer experience.Making positive effects on 5 oil wells by this method, instructive effect provide by the measure for controlling and decreasing eccentric wear of sucker rods.
Rotating Magnetic Fields Inhibit Mitochondrial Respiration, Promote Oxidative Stress and Produce Loss of Mitochondrial Integrity in Cancer Cells (2021)

Electromagnetic fields (EMF) raise intracellular levels of reactive oxygen species (ROS) that can be toxic to cancer cells. Because weak magnetic fields influence spin state pairing in redox-active radical electron pairs, we hypothesize that they disrupt electron flow in the mitochondrial electron transport chain (ETC). We tested this hypothesis by studying the effects of oscillating magnetic fields (sOMF) produced by a new noninvasive device involving permanent magnets spinning with specific frequency and timing patterns. We studied the effects of sOMF on ETC by measuring the consumption of oxygen (O<sub>2</sub>) by isolated rat liver mitochondria, normal human astrocytes, and several patient derived brain tumor cells, and O<sub>2</sub> generation/consumption by plant cells with an O<sub>2</sub> electrode. We also investigated glucose metabolism in tumor cells using <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance and assessed mitochondrial alterations leading to cell death by using fluorescence microscopy with MitoTracker™ and a fluorescent probe for Caspase 3 activation. We show that sOMF of appropriate field strength, frequency, and on/off profiles completely arrest electron transport in isolated, respiring, rat liver mitochondria and patient derived glioblastoma (GBM), meningioma and diffuse intrinsic pontine glioma (DIPG) cells and can induce loss of mitochondrial integrity. These changes correlate with a decrease in mitochondrial carbon flux in cancer cells and with cancer cell death even in the non-dividing phase of the cell cycle. Our findings suggest that rotating magnetic fields could be therapeutically efficacious in brain cancers such as GBM and DIPG through selective disruption of the electron flow in immobile ETC complexes.
A study of Linux Containers and their ability to quickly offer scalability for web services (2015)

The purpose of the thesis is to explore and investigate Linux container's ability to allow for quick scaling of web services.This is important for many web service providers, because there is a desire to keep a low amount of resources running when there are low amounts of traffic.This can have economical and environmental benefits.Being able to scale up the amount of resources quickly, is important when the traffic increases to keep within service level agreements and to keep the service running for the customers.Linux containers are a relatively new type of technology that allows for virtualization and running of applications.The focus of the thesis became to compare this new type of technology with a more commonly used technology, which is virtual machines based on templates.Two experiments were designed to test the two types of technologies.The first experiment wanted to test how long it would take to launch a new web server, while the second experiment was used to test how long it took to replace a web server with a newly launched database server.Kubernetes and Docker were the software used for Linux containers and MLN and Openstack were used for the virtual machine templates.The results showed a significant difference in relation to how quickly these two types of software combinations were able to scale.The results and analysis showed that Kubernetes was able to scale faster than MLN.Given the results, it became evident that Kubernetes should be chosen over MLN when considering quick scalability.Even though research and theory regarding Linux containers gives an indication of it being significantly faster than virtual machines and templates, it is impossible to definitely conclude that all software that uses Linux containers would perform better than software using virtual machines and templates.It is however likely to assume that it would be the case in most setups.iii My family: My parents, Kristine and Jan, and my
Abstract 2459: A Potentially Novel Role Of The Follistatin-activin Pathway In Heart Failure And Myocardial Recovery Following Lvad Combination Therapy (2007)

Background - Recent evidence suggests that the activin pathway may play an important role in the pathogenesis of heart failure. Follistatins are potent inhibitors of activin signalling but their role in the heart is unknown. The purpose of this study was to investigate myocardial expression of follistatin (FST) and follistatin-like (FSTL1 and FSTL3) in heart failure and following recovery in patients who received combined mechanical unloading using left ventricular assist device (LVAD) and pharmacologic therapy. Methods and results - Real time quantitative PCR was used to examine gene expression in myocardial samples from donors (n=9), end-stage heart failure patients (n=27) and patients who recovered from heart failure sampled at the time of LVAD implant, at the time of LVAD removal following recovery, and one year post explant. Both FSTL1 and FSTL3 were elevated in heart failure (1.51 and 2.50 fold respectively p<0.005) with expression returning to normal following recovery. Whereas FSTL3 correlated with molecular markers of disease severity, FSTL1 showed negative correlation with skeletal α-actin and positive correlation with the endothelial cell marker CD31, suggesting a potential link with extent of vascularization. FSTL1 expression levels at LVAD implant also correlated with significantly higher ejection fraction following recovery suggesting initial levels may have an influence on long term outcome. Immunohistochemical analysis showed that FST was localised in fibroblasts and endothelium, FSTL1 in myocytes, endothelium and smooth muscle cells, and FSLT3 in myocytes and endothelium. Microarray analysis showed that FST and FSTL1 expression correlate with extracellular matrix-related and calcium binding proteins, whereas FSTL3 is associated with cell signalling and transcription. Conclusion - These data show for the first time that elevated myocardial expression of follistatin-like genes is a feature of heart failure and that this may be linked both to disease severity and to mechanisms underlying recovery thereby revealing new insight into the pathogenesis of heart failure and offering novel therapeutic targets.
CD44 Monoclonal Antibody-Enhanced Clearance Of Chronic Myeloid Leukemia Stem Cells From The Malignant Niche (2013)

Abstract Introduction Leukemia stem cells (LSC) in chronic myeloid leukemia (CML) are important in disease progression and relapse. Conventional therapy with tyrosine kinase inhibitors (TKIs), although very effective at reducing bulk CML cells, frequently fail to eliminate LSC residing in the protective bone marrow niche. Thus, there is an unmet need for combination therapy that simultaneously targets bulk disease and eliminates LSC in order to prevent disease progression and relapse. Stem cell niches are often rich in hyaluronic acid. CD44, the main receptor for hyaluronic acid, and some of its splice variants are frequently overexpressed on cancer stem cells, including LSC. In this study we aimed to evaluate the in vivo anti-LSC activity of CD44 monoclonal antibody (mAb) RG7356. Methods and Results For anti-CD44 LSC inhibition studies, immunodeficient RAG2-/-γc-/- neonatal mice were intrahepatically transplanted with human BC LSC from imatinib responsive and resistant patients. Once engraftment was verified, mice were treated with control IgG1 (30mg/kg/week), dasatinib (25mg/kg/day), CD44 mAb (RG7356) (30mg/kg/week) or a combination of CD44 mAb (RG7356) and dasatinib. The mice were sacrificed after two weeks of therapy and bone marrow, spleen, peripheral blood and myeloid sarcomas were analyzed by FACS to assess CML LSC burden. In the imatinib responsive patient, CD44 mAb single agent therapy showed an 85% reduction of LSC in bone marrow, 98% reduction in spleen and 91% reduction in peripheral blood. CD44 mAb therapy thus showed an efficacy equal to that of dasatinib, which showed an 87%, 98% and 98% reduction in bone marrow, spleen and peripheral blood respectively. Combination therapy (CD44 mAb + dasatinib) completely eradicated any trace of CML LSC in all tissues analyzed. In the imatinib resistant patient, dasatinib very effectively reduced the number of myeloid sarcomas and blasts but did not inhibit LSC survival in bone marrow. In contrast, CD44 mAb single agent therapy effectively reduced the number of CML LSC to 51% in bone marrow, 96% in spleen and 93% in peripheral blood. Conclusions This study demonstrates that a human CD44 specific mAb, RG7356, significantly reduces CML LSC survival. Notably, TKI resistance of CML LSC, can be overcome by treatment with a human CD44 specific mAb, RG7356, as it sensitizes CML LSC residing in malignant niches to dasatinib. From these results, RG7356 appears to be an excellent antibody for future combination clinical studies aimed at eradicating CML. Disclosures: Runza: employee of Roche Diagnostics GmbH: Employment. Weigand:Roche Diagnostics GmbH: Employment. Jamieson:J&J: Research Funding; Sanofi: Consultancy; Roche: Research Funding.
Temperature-dependent internal friction in silicon nanoelectromechanical systems (2000)

We report the temperature-dependent mechanical properties of nanofabricated silicon resonators operating in the megahertz range. Reduction of temperature leads to an increase of the resonant frequencies of up to 6.5%. Quality factors as high as 1000 and 2500 are observed at room temperature in metallized and nonmetallized devices, respectively. Although device metallization increases the overall level of dissipation, internal friction peaks are observed in all devices in the T=160–180 K range.
Facilitating Trust in Privacy-Preserving E-Learning Environments (2011)

This research explores a new model for facilitating trust in online e-learning activities. We begin by protecting the privacy of learners through identity management (IM), where personal information can be protected through some degree of participant anonymity or pseudonymity. In order to expect learners to trust other pseudonymous participants, we realize that a reliable mechanism is needed for managing participants' reputations and assuring that such reputations are legitimately obtained. Further, because participants can hold multiple identities or can adopt new pseudonymous personas, a reliable and trustworthy mechanism for reputation transfer (RT) from one persona to another is required. Such a reputation transfer model must preserve privacy and at the same time prevent linkability of learners' identities and personas. In this paper, we present a privacy-preserving reputation management (RM) system which allows secure transfer of reputation. A prototypical implementation of our reputation transfer protocol and the successful experimental deployment of our reputation management solution in an e-learning discussion forum serve as a proof of concept.
On the Natural Convection in a Cavity With a Cooled Top Wall and Multiple Protruding Heaters (1995)

Natural convection in rectangular enclosures with multiple protruding heaters mounted on one side wall is of relevance to the cooling of electronic equipment. In some configurations, the top wall behaves as the heat sink while the opposing vertical wall and the bottom wall are insulated. The present work examines the peculiarities introduced in the natural convection process for such configurations. The enclosure considered had five protrusions, cavity width to heater length ratio of 1.2 and cavity height to heater length ratio of 11. It is shown that for such configurations, a stable flow exists only at lower Rayleigh numbers and that above a certain critical Rayleigh number, only quasi-steady solutions exist. At low Rayleigh numbers(Ra* ≤ 1.5 × 107), the flow is stable and characterized by the presence of a primary flow cell and a counter-rotating secondary cell at the top of the enclosure. At higher Rayleigh numbers (Ra* ≥ 3 × 108), however, the isothermal top wall causes a periodic flow pattern to develop within the enclosure. Several interesting characteristics of the flow and temperature fields are presented. Results compared with previous experimental and numerical work are found to be in good agreement.
Peptidoglycan synthesis and structure in Staphylococcus haemolyticus expressing increasing levels of resistance to glycopeptide antibiotics (1996)

The structures of cytoplasmic peptidoglycan precursor and mature peptidoglycan of an isogenic series of Staphylococcus haemolyticus strains expressing increasing levels of resistance to the glycopeptide antibiotics teicoplanin and vancomycin (MICs, 8 to 32 and 4 to 16 microg/ml, respectively) were determined. High-performance liquid chromatography, mass spectrometry, amino acid analysis, digestion by R39 D,D-carboxypeptidase, and N-terminal amino acid sequencing were utilized. UDP-muramyl-tetrapeptide-D-lactate constituted 1.7% of total cytoplasmic peptidoglycan precursors in the most resistant strain. It is not clear if this amount of depsipeptide precursor can account for the levels of resistance achieved by this strain. Detailed structural analysis of mature peptidoglycan, examined for the first time for this species, revealed that the peptidoglycan of these strains, like that of other staphylococci, is highly cross-linked and is composed of a lysine muropeptide acceptor containing a substitution at its epsilon-amino position of a glycine-containing cross bridge to the D-Ala 4 of the donor, with disaccharide-pentapeptide frequently serving as an acceptor for transpeptidation. The predominant cross bridges were found to be COOH-Gly-Gly-Ser-Gly-Gly-NH2 and COOH-Ala-Gly-Ser-Gly-Gly-NH2. Liquid chromatography-mass spectrometry analysis of the peptidoglycan of resistant strains revealed polymeric muropeptides bearing cross bridges containing an additional serine in place of glycine (probable structures, COOH-Gly-Ser-Ser-Gly-Gly-NH2 and COOH-Ala-Gly-Ser-Ser-Gly-NH2). Muropeptides bearing an additional serine in their cross bridges are estimated to account for 13.6% of peptidoglycan analyzed from resistant strains of S. haemolyticus. A soluble glycopeptide target (L-Ala-gamma-D-iso-glutamyl-L-Lys-D-Ala-D-Ala) was able to more effectively compete for vancomycin when assayed in the presence of resistant cells than when assayed in the presence of susceptible cells, suggesting that some of the resistance was directed towards the cooperativity of glycopeptide binding to its target. These results are consistent with a hypothesis that alterations at the level of the cross bridge might interfere with the binding of glycopeptide dimers and therefore with the cooperative binding of the antibiotic to its target in situ. Glycopeptide resistance in S. haemolyticus may be multifactorial.
The Possibility of Compensating Astronomical Seeing (1953)

The severe limitations imposed upon nearly all astronomical observations by the effects resulting from passage of light rays through the turbulent atmosphere of the earth are familiar to every observer. With a small instrument the effect may appear largely as a continual shifting and scintillation of the of a star, but with a large telescope poor usually manifests itself mainly as an enlargement and blurring of the image. The reason for this difference becomes apparent when one makes a knife-edge test with a large telescope on a bright star. The turbulent elements of the atmosphere appear as shifting dark and bright areas on the of the objective. In general these turbulent elements or waves have dimensions of the order of a foot or two small compared to the aperture of a large reflector. Thus the integrated effect of a number of turbulent elements leads to an unsteady enlargement of the image, perhaps with some irregular shifting in position. The seriousness of this is evident when one realizes that ideally the 200-inch Hale telescope is capable of giving diffraction images of stars about %0 oi a second of arc in diameter, yet the size of the seeing image produced is in the range j4 second to perhaps 5 or 10 seconds, being about 2 seconds in diameter on the average. Only rarely are images as small as yi second observed, and one may consider himself fortunate to experience one hour out of 1000 of the finest seeing, even at the best locations.
Relation of Arginine-Lysine Antagonism to Herpes simplex Growth in Tissue Culture (1981)

In the studies conducted, arginine deficiency suppressed herpes simplex virus replication in tissue culture. Lysine, an analog of arginine, as an antimetabolite, antagonized the viral growth-promoting action of arginine. The in vitro data may be the basis for the observation that patients prone to herpetic lesions and other related viral infections, particularly during periods of stress, should abstain from arginine excess and may also require supplemental lysine in their diet.
Communicating with databases in natural language (1984)

In the first chapters is a full description of natural languages and interface to relational database. Natural language processing and the use of PROLOG are discussed. Features include also a practical discussion of parsing natural language with accompanying programs in PROLOG
Degradation of macrolide-lincosamide-streptogramin antibiotics by Lactobacillus strains from animals. (1981)

Ability to degrade macrolide, lincosamide and streptogramin antibiotics was noted in Lactobacillus strains isolated from the caeca of pigs, cattle and poultry. Some of the strains degraded all the three classes of antibiotics while some degraded either macrolides and lincosamides or macrolidese and streptogramins. There were also strains which degraded only macrolide or streptogramin type A antibiotics. Strains with any of these degradation abilities were always resistant to the antibiotics concerned, but strains with resistance to these antibiotics were often, but not always able to degrade them.
The Detection of Epstein-Barr Virus DNA in Lung Tissue from Patients with Idiopathic Pulmonary Fibrosis (1999)

Idiopathic pulmonary fibrosis (IPF) is a clinical syndrome in which the precipitating factors are unclear. An association between Epstein-Barr Virus (EBV) and IPF had previously been suggested using serology and immunohistochemistry. This study sought confirmation of the presence of EBV DNA in the lung tissue of patients with IPF. Lung tissue obtained surgically from 27 patients with IPF and 28 control subjects was investigated for the presence of EBV by immunohistochemistry and polymerase chain reaction (PCR) analysis. Immunohistochemistry used antibodies specific for EBV lytic cycle antigens (gp340/220 and VCA). Nested PCR analysis used oligonucleotide primers specific for EBV and was sensitive to one copy of EBV DNA. Twelve of the 27 patients with IPF (44%) and three of the 28 control subjects (10%) were EBV positive by immunohistochemistry (p = 0.005). Thirteen of the patients with IPF (48%) and four of the control subjects (14%) were EBV positive by PCR (p = 0.007). Eleven of the patients with IPF (41%) and none of the control subjects were EBV positive by both immunohistochemistry and PCR (p = < 0.001). These data further suggest an association between EBV and IPF. In addition it defines a novel method for detecting EBV in lung tissue. EBV may be involved in the pathogenesis of the disease; however, further studies are required to establish a causal relationship.