gebhart/patrepeval huggingface dataset:
Detection of cytomegalovirus antibodies by an enzyme-linked immunosorbent assay using recombinant polypeptides of the large phosphorylated tegument protein pp150 (1992)

Parts of the large phosphorylated tegument protein, pp150, of human cytomegalovirus (HCMV) were expressed in bacteria. The resulting fusion proteins were tested in a Western blot (immunoblot) assay for reactivity with a monoclonal antibody against pp150, with a polyspecific rabbit antiserum, and with human reconvalescent-phase sera. Those fusion proteins that performed well in the Western blot assay were used as antigens in enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies against HCMV. Five different recombinant beta-galactosidase fusion proteins were evaluated by ELISA using 62 seropositive and 38 seronegative human serum samples. Of all the proteins tested, one peptide representing 162 amino acids of pp150 was superior to the others with regard to sensitivity and specificity. All sera known to be positive for antibodies against HCMV were identified by combining the results of the ELISAs with the different pp150 fusion proteins. Therefore, it appears that peptides from a single protein of HCMV might be sufficient to identify HCMV-seropositive individuals by recombinant ELISA.
Correction for distortion of echo‐planar images used to calculate the apparent diffusion coefficient (1996)

Abstract An algorithm for correcting the distortions that occur in diffusion‐weighted echo‐planar images due to the strong diffusion‐sensitizing gradients is presented. The dominant distortions may be considered to be only changes of scale coupled with a shear and linear translation in the phase‐encoding direction. It is then possible to correct for them by using an algorithm in which each line of the image in the phase‐encoding direction is considered in turn, with only one parameter (the scale) to be found by searching.
A two-dimensional direct-coupled standing-wave oscillator array (2013)

This paper presents a 2-D direct-coupled standing-wave oscillator (SWO) array implemented in a 90nm CMOS technology with 61.5GHz oscillation frequency. The SWO array can provide synchronous signals with identical frequencies, amplitudes, and phases at multiple locations over a chip. The reported SWO array is a reticular structure in a plane, and therefore it can be extended in two dimensions periodically in a CMOS process. Wireless measurement is a feasible solution to verify the synchronicity of the reported 2-D SWO at mm-wave regime. A mm-wave radiator which consists of the proposed SWO array, an RF driver array, and an on-chip loop antenna array is implemented in a single chip for wireless measurement purpose. The indirect evidence of synchronicity is provided from the correlation between the wireless measured effective isotropic radiated power (EIRP) and phase noise of 1 × 1, 2 × 2, and 3 × 3 arrays. The experimental and theoretical results are matched. The EIRP in the normal direction of the array is increasing by a factor of 10 log N <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and the phase noise at 1MHz offset is reducing by a factor of 10 log N over that of a single cell, where N is the number of unit cells in the array.
Long-lived keratin 15+ esophageal progenitor cells contribute to homeostasis and regeneration (2017)

The esophageal lumen is lined by a stratified squamous epithelium comprised of proliferative basal cells that differentiate while migrating toward the luminal surface and eventually desquamate. Rapid epithelial renewal occurs, but the specific cell of origin that supports this high proliferative demand remains unknown. Herein, we have described a long-lived progenitor cell population in the mouse esophageal epithelium that is characterized by expression of keratin 15 (Krt15). Genetic in vivo lineage tracing revealed that the Krt15 promoter marks a long-lived basal cell population able to self-renew, proliferate, and generate differentiated cells, consistent with a progenitor/stem cell population. Transcriptional profiling demonstrated that Krt15+ basal cells are molecularly distinct from Krt15- basal cells. Depletion of Krt15-derived cells resulted in decreased proliferation, thereby leading to atrophy of the esophageal epithelium. Further, Krt15+ cells were radioresistant and contributed to esophageal epithelial regeneration following radiation-induced injury. These results establish the presence of a long-lived and indispensable Krt15+ progenitor cell population that provides additional perspective on esophageal epithelial biology and the widely prevalent diseases that afflict this epithelium.
Cost-effective surgical registration using consumer depth cameras (2016)

The high costs associated with technological innovation have been previously identified as both a major contributor to the rise of health care expenses, and as a limitation for widespread adoption of new technologies. In this work we evaluate the use of two consumer grade depth cameras, the Microsoft Kinect v1 and 3DSystems Sense, as a means for acquiring point clouds for registration. These devices have the potential to replace professional grade laser range scanning devices in medical interventions that do not require sub-millimetric registration accuracy, and may do so at a significantly reduced cost. To facilitate the use of these devices we have developed a near real-time (1-4 sec/frame) rigid registration framework combining several alignment heuristics with the Iterative Closest Point (ICP) algorithm. Using nearest neighbor registration error as our evaluation criterion we found the optimal scanning distances for the Sense and Kinect to be 50-60cm and 70-80cm respectively. When imaging a skull phantom at these distances, RMS error values of 1.35mm and 1.14mm were obtained. The registration framework was then evaluated using cranial MR scans of two subjects. For the first subject, the RMS error using the Sense was 1.28 ± 0.01 mm. Using the Kinect this error was 1.24 ± 0.03 mm. For the second subject, whose MR scan was significantly corrupted by metal implants, the errors increased to 1.44 ± 0.03 mm and 1.74 ± 0.06 mm but the system nonetheless performed within acceptable bounds.
Contour based visualization of vessel movement predictions (2014)

We present a visualization method for the interactive exploration of predicted positions of moving objects, in particular, ocean-faring vessels. Two simple prediction models, one based on similarity to historical trajectories and one on Monte Carlo simulation, are presented. The prediction models generate temporal probability density fields starting from a known situation. We use contours to visualize spatio-temporal zones of these density fields. Predictions are split into a configurable number of segments for which we render one or more contours. Users, investigating and exploring the possible development of a situation, can see where a vessel will be in the near future according to a given prediction model. Through a number of real-world use cases and a discussion with users, we show our methods can be used in monitoring traffic for collision avoidance, and detecting illegal activities, like piracy or smuggling. By applying our methods to pedestrian movements, we show that our methods can also be applied to a different domain. Keywords: situational awareness; uncertainty; vessel prediction; visualization
Inhibitor Discovery of Full-Length New Delhi Metallo-β-Lactamase-1 (NDM-1) (2013)

New Delhi metallo-β-lactmase-1 (NDM-1) has recently attracted extensive attention for its biological activities to catalyze the hydrolysis of almost all of β-lactam antibiotics. To study the catalytic property of NDM-1, the steady-kinetic parameters of NDM-1 toward several kinds of β-lactam antibiotics have been detected. It could effectively hydrolyze most β-lactams (k cat/K m ratios between 0.03 to 1.28 µmol⁻¹.s⁻¹), except aztreonam. We also found that thiophene-carboxylic acid derivatives could inhibit NDM-1 and have shown synergistic antibacterial activity in combination with meropenem. Flexible docking and quantum mechanics (QM) study revealed electrostatic interactions between the sulfur atom of thiophene-carboxylic acid derivatives and the zinc ion of NDM-1, along with hydrogen bond between inhibitor and His189 of NDM-1. The interaction models proposed here can be used in rational design of NDM-1 inhibitors.
Biocatalytic Evolution of a Biocatalyst Marker: Towards the Ultrasensitive Detection of Immunocomplexes and DNA Analysis (2006)

Sensitive ELISA: A new enzyme-labeled immunosorbant assay (ELISA) protocol has been developed that makes use of two enzymatic reactions to detect antibody–antigen interactions: 1) the conversion of prothrombin to thrombin by ecarin and 2) the hydrolysis of a nonfluorescent substrate 1 to a fluorescent product 2 by thrombin. The system was used to detect bovine serum albumin, telomerase, and DNA with high sensitivity.
Comparison of Glycol with Silicon Oil as a Heat Transfer Medium (1997)

An investigation has been mado to compare the glycol aqueous solution with silicon oil used as a heat transfer medium. Detailed analysis has been made in the aspects of physical properties, prices, flow resistances, heat transfer coefficients and specific heats. This study showed that the glycol fluids have advantages as secondary fluid over the silicon oil because of low cost, better heat transfer performance and lower flow resistance.
A fully-monolithic SiGe differential voltage-controlled oscillator for 5 GHz wireless applications (2002)

A fully integrated and differential SiGe VCO was designed for 5 GHz wireless applications. The measured phase noise is -98 dBc/Hz at 100 kHz offset off the 5 GHz carrier. It has a tuning range of 12.3% with a control voltage from 0 to 3 V, and a figure of merit of more than -180 dBc/Hz, The current drawn from 3 V is 5 mA for the core and 2.2 mA for the output buffers.
Behavior of composite rigid frame bridge under bi-directional seismic excitations (2014)

Pushover analysis and time history analysis are conducted to explore the bi-directional seismic behavior of composite steel-concrete rigid frame bridge, which is composed of RC piers and steel-concrete composite girders. Both longitudinal and transverse directions excitations are investigated using OpenSees. Firstly, the applicability of pushover analysis based on the fundamental mode is discussed. Secondly, an improved pushover analysis method considering the contribution of higher modes is proposed, and the applicability on composite rigid frame bridges under bi-directional earthquake is verified. Based on this method, an approach to predict the displacement responses of composite rigid frame bridge under random bi-directional seismic excitations by revising the elasto-plastic demand curve is also proposed. It is observed that the developed method yield a good estimate on the responses of composite rigid frame bridges under bi-directional seismic excitations.
Asymmetric Cyclization of Achiral Olefinic Organolithiums Controlled by a Stereogenic Lithium:  Intramolecular Carbolithiation in the Presence of (−)-Sparteine (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTAsymmetric Cyclization of Achiral Olefinic Organolithiums Controlled by a Stereogenic Lithium: Intramolecular Carbolithiation in the Presence of (−)-SparteineWilliam F. Bailey and Michael J. MealyView Author Information Department of Chemistry, The University of Connecticut Storrs, Connecticut 06269-3060 Cite this: J. Am. Chem. Soc. 2000, 122, 28, 6787–6788Publication Date (Web):June 27, 2000Publication History Received4 February 2000Published online27 June 2000Published inissue 1 July 2000https://pubs.acs.org/doi/10.1021/ja000471lhttps://doi.org/10.1021/ja000471lrapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views1069Altmetric-Citations101LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cyclization,Ethers,Isomerization,Ligands,Stereoselectivity Get e-Alerts
Two Layered PKI Model for Device Authentication in Multi-Domain Home Networks (2006)

With the growing number of home network deployment, the security issues in home networks have been focused on as one of the challenging problems. We present a multi-domain device registration and authentication model that uses two-layered PKI approach in order to make the communication efficient and user intervention minimized. The two layers of our model are Global PKI layer and Localized PKI layer. Global PKI layer use conventional PKI model. There are only one global root CA and certificate verification is performed by validating the certificate-chain linked to the root CA. Otherwise, in Localized PKI layer, each home gateway take a role of root CA which is responsible for issuing device certificates to the devices belong to its domain. We use Global PKI layer for device registration and authentication of inter-home-network, but use Localized PKI layer to authenticate each end-device. Based on this separating, our model provides secure, efficient and user friendly multi-domain device authentication protocols.
Electrical characterization of GaN/SiC n-p heterojunction diodes (1998)

GaN/SiC heterojunction diodes have been fabricated and characterized. Epitaxial n-type GaN films were grown using metalorganic chemical vapor deposition (MOCVD) and electron cyclotron resonance assisted molecular beam epitaxy (ECR-MBE) on p-type Si-face 6H-SiC wafers. The I–V characteristics have diode ideality factors and saturation currents as low as 1.2 and 10−32 A/cm2, respectively. The built-in potential in the MOCVD- and ECR-MBE-grown n-p heterojunctions was determined from capacitance–voltage measurements at 2.90±0.08 eV and 2.82±0.08 eV, respectively. From the built-in potential the energy band offsets for GaN/SiC heterostructures are determined at ΔEC=0.11±0.10 eV and ΔEV=0.48±0.10 eV.
Sleep Spindles as an Electrographic Element: Description and Automatic Detection Methods (2016)

Sleep spindle is a peculiar oscillatory brain pattern which has been associated with a number of sleep (isolation from exteroceptive stimuli, memory consolidation) and individual characteristics (intellectual quotient). Oddly enough, the definition of a spindle is both incomplete and restrictive. In consequence, there is no consensus about how to detect spindles. Visual scoring is cumbersome and user dependent. To analyze spindle activity in a more robust way, automatic sleep spindle detection methods are essential. Various algorithms were developed, depending on individual research interest, which hampers direct comparisons and meta-analyses. In this review, sleep spindle is first defined physically and topographically. From this general description, we tentatively extract the main characteristics to be detected and analyzed. A nonexhaustive list of automatic spindle detection methods is provided along with a description of their main processing principles. Finally, we propose a technique to assess the detection methods in a robust and comparable way.
Role of the clotting system in the pathogenesis of neuroimmunologic disease. (1987)

Experimental allergic encephalomyelitis (EAE) is a prototypic neuroautoimmune disease involving sensitization to central nervous system myelin basic protein (MBP). Our studies of the clotting system and ensuing fibrinolysis implicate coagulation and cleavage of fibrin within or on the luminal surface of the cerebrovasculature as events initiating the inflammation characterizing EAE. Among recipient rats injected with MPB-primed, cultured-activated lymph node cells, opening of the blood-brain barrier (BBB) and deposition of perivascular fibrin within the spinal cord occur in parallel 1 day before onset of clinical signs of EAE. Daily treatment of recipient rats with trans-4-(aminomethyl)cyclohexanecarboxylic acid, a synthetic product that specifically inhibits plasminogen activator derived from endothelial cells, results in marked reduction of increased permeability of the BBB and suppression of clinical signs of EAE. We postulate that the critical event precipitating EAE is binding of circulating MBP-reactive immune effector cells to MBP immunodeterminants on the surface of cerebrovascular endothelial cells. Coagulation and ensuing fibrinolysis occur at sites of binding of effector cells to cerebrovascular endothelium. Release of biologically active peptides cleaved from fibrin open the BBB, thereby setting the stage for the cascade of inflammatory events culminating in clinical manifestations of EAE.
Bright betatron X-ray radiation from a laser-driven-clustering gas target (2013)

Hard X-ray sources from femtosecond (fs) laser-produced plasmas, including the betatron X-rays from laser wakefield-accelerated electrons, have compact sizes, fs pulse duration and fs pump-probe capability, making it promising for wide use in material and biological sciences. Currently the main problem with such betatron X-ray sources is the limited average flux even with ultra-intense laser pulses. Here, we report ultra-bright betatron X-rays can be generated using a clustering gas jet target irradiated with a small size laser, where a ten-fold enhancement of the X-ray yield is achieved compared to the results obtained using a gas target. We suggest the increased X-ray photon is due to the existence of clusters in the gas, which results in increased total electron charge trapped for acceleration and larger wiggling amplitudes during the acceleration. This observation opens a route to produce high betatron average flux using small but high repetition rate laser facilities for applications.
Charge Carriers Removal from 4H-SiC Using Field Enhanced by Optical Activation Diffusion Method (2002)

This paper presents results of charge carriers removal from 4H-SiC wafers. A new chemical reactor has been tested for forced diffusion purification (reversed diffusion) of SiC films. Different types of conditions have been used to purify SiC samples. A 5 mW (630-680 nm) laser has been used to improve results. I-V characteristic curves have been measured to verify changes in electrical properties of the samples. SIMS has been used to analyze the concentrations of impurities in the SiC samples before and after treatment. It has been demonstrated that the field enhanced by optical activation diffusion method can remove impurities such as N and B form SiC films. As a result, the electrical properties of the SiC wafers have been significantly improved during treatment, especially in cases where a laser is emplaced.
Microstructured Optical Fibers—Fundamentals and Applications (2005)

In recent years optical fibers having a complex microstructure in the transverse plane have attracted much attention from both researchers and industry. Such fibers can either guide light through total internal reflection or the photonic bandgap effect. Among the many unique applications offered by these fibers are mode guidance in air, highly flexible dispersion engineering, and the use of very heterogeneous material combinations. In this paper, we review the different types and applications of microstructured optical fibers, with particular emphasis on recent advances in the field.
Natural paucity of anaplerotic enzymes: basis for dependence of Arthrobacter pyridinolis on L-malate for growth (1976)

Previous work has shown that in Arthrobacter pyridinolis the transport systems for glucose and several amino acids are respiration coupled, with malate oxidation occurring concomitantly with transport. The requisite malate has to be supplied exogenously, so that growth on glucose or certain amino acids only occurs if malate is also present in the medium. These and other data suggested that A. pyridinolis might be deficient in anaplerotic enzymes, which maintain intracellular levels of dicarboxylic acids. A comparative study was undertaken of anaplerotic enzymes in A. pyridinolis and in a closely related species, A. crystallopoietes, which has respiration-coupled transport of glucose but can grow on glucose without added malate. The paucity of anaplerotic enzymes in A. pyridinolis and its probable relationship to the malate requirement for growth on glucose were documented as follows: (i) A. crystallopoietes, but not A. pyridinolis, possesses phosphoenolpyruvate carboxylase activity, and neither species contains pyruvate carboxylase; (ii) both A. pyridinolis and A. crystallopoietes possess glyoxylate pathways that are induced by acetate but not by hexoses; (iii) isocitrate lyase-deficient mutants of A. pyridinolis fail to grow on rhamnose and fructose as well as acetate; and (iv) mutants of A. crystallopoietes that require malate for growth on glucose are deficient in phosphoenolpyruvate carboxylase.
Voice conversion based on static speaker characteristics (2002)

Voice conversion has recently emerged as an interesting branch of speech processing that deals with the modification of a speaker's perceived identity. This technology has applications in speech recognition, the entertainment and security industries. This paper provides a brief introduction to current voice conversion approaches, and discusses the development of the PASS system, a parametric voice conversion algorithm based on static speaker characteristics. The system is easy to implement, requires no phonetic transcription of the speech data, and is shown to be valuable in the case where very little training data is available. Particular mention is made of the pitch extraction subsystem, which uses a novel pitch determination algorithm to ensure the robust estimation of pitch statistics.