gebhart/patrepeval huggingface dataset:
Orally Administered Thermostable <i>N</i> -Acyl Homoserine Lactonase from Bacillus sp. Strain AI96 Attenuates Aeromonas hydrophila Infection in Zebrafish (2012)

N-Acylated homoserine lactone (AHL) lactonases are capable of degrading signal molecules involved in bacterial quorum sensing and therefore represent a new approach to control bacterial infection. Here a gene responsible for the AHL lactonase activity of Bacillus sp. strain AI96, 753 bp in length, was cloned and then expressed in Escherichia coli. The deduced amino acid sequence of Bacillus sp. AI96 AiiA (AiiA(AI96)) is most similar to those of other Bacillus sp. AHL lactonases (~80% sequence identity) and was consequently categorized as a member of the metallo-β-lactamase superfamily. AiiA(AI96) maintains ~100% of its activity at 10°C to 40°C at pH 8.0, and it is very stable at 70°C at pH 8.0 for at least 1 h; no other Bacillus AHL lactonase has been found to be stable under these conditions. AiiA(AI96) resists digestion by proteases and carp intestinal juice, and it has broad-spectrum substrate specificity. The supplementation of AiiA(AI96) into fish feed by oral administration significantly attenuated Aeromonas hydrophila infection in zebrafish. This is the first report of the oral administration of an AHL lactonase for the efficient control of A. hydrophila.
<title>Overwrite characteristics of magneto-optical disks by magnetic field modulation</title> (1990)

We have been experimenting with overwriting by magnetic field modulation using flying magnetic heads and single-sided disks. We developed a new protective layer for the disks. This is a spin-coated polymer layer containing α-Fe<sub>2</sub>0<sub>3</sub> powder. It holds lubricating oil well and increases durability. We obtained good read-write characteristics using the protective layer on a reflective structure disk.
Transrectal ultrasound guided manipulation of the canine prostate with minimum intervention (1997)

The canine prostate is a widely used and well established animal model for the assessment of therapeutic effects of laser technology in the search for better options for the treatment of benign prostatic hyperplasia. Conventionally for such experiments, the canine prostate is approached by laparotomy or transperineal urethrotomy. We have demonstrated a technique involving the use of ultrasound guided biopsy of the liver and prostate, and percutaneous laser treatment of the prostate, which has proved to be effective in reducing adverse effects on the experimental animals but without compromising scientific requirements for the experiments. We conclude that state-of-the-art percutaneous procedures not only refine animal intervention significantly but are also technically feasible for most laser studies using the canine prostate as an experimental model in the live animal.
Proton beams from intense laser-solid interaction: Effects of the target materials (2020)

We report systematic studies of laser-driven proton beams produced with micrometer-thick solid targets made of aluminum and plastic, respectively. Distinct effects of the target materials are found on the total charge, cutoff energy, and beam spot of protons in the experiments, and these are described well by two-dimensional particle-in-cell simulations incorporating intrinsic material properties. It is found that with a laser intensity of 8 × 1019 W/cm2, target normal sheath acceleration is the dominant mechanism for both types of target. For a plastic target, the higher charge and cutoff energy of the protons are due to the greater energy coupling efficiencies from the intense laser beams, and the larger divergence angle of the protons is due to the deflection of hot electrons during transport in the targets. We also find that the energy loss of hot electrons in targets of different thickness has a significant effect on the proton cutoff energy. The consistent results obtained here further narrow the gap between simulations and experiments.
Diode-pumped 1-W continuous-wave Er:YAG 3-?m laser (1999)

We have demonstrated 1.15 W of Gaussianlike (M(2) = 2) cw output at 2.94 mum from a diode laser end-pumped monolithic laser crystal composed of Er-doped yttrium aluminum garnet (YAG) bonded to undoped YAG. The laser was pumped with two polarization-coupled 2.5-W flared laser diodes that provided a 45-mum pump waist with a low N.A. (<0.04) . Output at 2.94 mum was generated with a 34% slope efficiency and a greater-than-unity quantum efficiency.
Codon 72 polymorphism of the TP53 gene (1990)

Journal Article Codon 72 polymorphism of the TP53 gene Get access S. Ara, S. Ara Search for other works by this author on: Oxford Academic PubMed Google Scholar P.S.Y. Lee, P.S.Y. Lee Search for other works by this author on: Oxford Academic PubMed Google Scholar M.F. Hansen, M.F. Hansen 1Department of Neuro-Oncology and Department of Molecular Genetics, The University of Texas M.D.Anderson Cancer CenterHouston, TX 77030, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar H. Saya H. Saya * *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 18, Issue 16, 25 August 1990, Page 4961, https://doi.org/10.1093/nar/18.16.4961 Published: 25 August 1990
A Robust Audio Watermark Representation Based on Linear Chirps (2006)

In this paper, we introduce a novel watermark representation for audio watermarking, where we embed linear chirps as watermark signals. Different chirp rates, i.e., slopes on the time–frequency (TF) plane, represent watermark messages such that each slope corresponds to a unique message. These watermark signals, i.e., linear chirps, are embedded and extracted using an existing watermarking algorithm. The extracted chirps are then postprocessed at the receiver using a line detection algorithm based on the Hough–Radon transform (HRT). The HRT is an optimal line-detection algorithm, which detects directional components that satisfy a parametric constraint equation in the image of a TF plane, even at discontinuities corresponding to bit errors. Simulation results show that HRT correctly detects the embedded watermark message after common signal processing operations for bit error rates up to 20%. The new watermark representation and the postprocessing stage based on HRT significantly improve the performance of the watermark detection process and can be combined with existing watermark embedding/extraction algorithms for increased robustness.
<scp>L</scp>‐DOPA‐induced dyskinesia in adult rats with a unilateral 6‐OHDA lesion of dopamine neurons is paralleled by increased c‐fos gene expression in the subthalamic nucleus (2006)

Levodopa (L-DOPA), the metabolic precursor of dopamine, is widely used as a pharmacological agent for the symptomatic treatment of Parkinson's disease. However, long-term L-DOPA use results in abnormal involuntary movements such as dyskinesias. There is evidence that abnormal cell signaling in the basal ganglia is involved in L-DOPA-induced dyskinesia. The subthalamic nucleus (STN) plays a key role in the circuitry of the basal ganglia and in the pathophysiology of Parkinson's disease. However, the contribution of the STN to L-DOPA-induced dyskinesias remains unclear. The objective of this work was to study the effects of acute or chronic systemic administration of L-DOPA to adult rats with a unilateral 6-hydroxydopamine (6-OHDA) lesion of dopamine neurons on c-fos expression in the STN and test the hypothesis that these effects correlate with L-DOPA-induced dyskinesias. c-fos mRNA expression was measured in the STN by in situ hybridization histochemistry at the single cell level. Our results confirm earlier evidence that the chronic administration of L-DOPA to rats with a unilateral 6-OHDA lesion increases c-fos expression in the STN. We also report that c-fos expression can be increased following an acute injection of L-DOPA to 6-OHDA-lesioned rats but not following a chronic injection of L-DOPA to sham-operated, unlesioned rats. Finally, we provide evidence that the occurrence and severity of dyskinesia is correlated with c-fos mRNA levels in the ipsilateral STN. These results suggest that altered cell signaling in the STN is involved in some of the behavioral effects induced by systemic L-DOPA administration.
Comparison of Three D-Dimer Assays for the Diagnosis of DVT: ELISA, Latex and an Immunofiltration Assay (NycoCard D-Dimer) (1994)

Ninety-two consecutive patients referred for suspicion of deep venous thrombosis (DVT) were analyzed for D-dimer using ELISA, latex test, and a new immunofiltration method (NycoCard D-Dimer). Contrast venography verified the diagnosis in 40, and excluded the diagnosis in 52 patients. The sensitivity, negative predictive values, specificity and positive predictive values were, for ELISA 98%, 95%, 38% and 54, for NycoCard D-Dimer 100%, 100%, 42% and 57% and for the latex test 73%, 78%, 75%, and 69%, respectively. Sensitivity and specificity were inversely related with increasing pathological cut-off value. Comparison of test results by concentration category revealed a good agreement between ELISA and NycoCard D-Dimer, but to less extent between latex and the two other tests. It is concluded that NycoCard D-Dimer and D-dimer ELISA are well-suited as exclusion tests for DVT. A plasma sample is tested with NycoCard D-Dimer in less than 2 min. Thus, this test combines advantageous analytical properties comparable to the ELISA-test, with rapidity and simplicity comparable to the latex test.
PWM AC chopper control of single-phase induction motor for variable-speed fan application (2008)

This work presents a variable speed control method for fan applications. A pulse-width-modulated (PWM) AC chopper changes the effective value of the supply voltage applied to a single-phase induction motor. This variable supply voltage gives the ability to control the speed of the motor. Harmonics generated by the speed control unit are filtered by an input filter according to EN 61000-3-2 limits for harmonic current emissions standards. Experimental results on a 230 V, 210 W in-line centrifugal fan for exhaust ventilation application shows that PWM AC chopper is simple and cost effective control method compared to other methods in terms of simplicity and input harmonic content.
Occurrence of a 2-bp (AT) deletion allele and a nonsense (G-to-T) mutant allele at the E2 (DBT) locus of six patients with maple syrup urine disease: multiple-exon skipping as a secondary effect of the mutations. (1993)

We have identified two novel mutant alleles in the transacylase (E2) gene of the human branched-chain alpha-keto acid dehydrogenase (BCKAD) complex in 6 of 38 patients with maple syrup urine disease (MSUD). One mutation, a 2-bp (AT) deletion in exon 2 of the E2 gene, causes a frameshift downstream of residue (-26) in the mitochondrial targeting presequence. The second mutation, a G-to-T transversion in exon 6 of the E2 gene, produces a premature stop codon at Glu-163 (E163*). Transfection of constructs harboring the E163* mutation into an E2-deficient MSUD cell line produced a truncated E2 subunit. However, this mutant E2 chain is unable to assemble into a 24-mer cubic structure and is degraded in the cell. The 2-bp (AT) deletion and the E163* mutant alleles occur in either the homozygous or compound-heterozygous state in the 6 of 38 unrelated MSUD patients studied. Moreover, an array of precise single- and multiple-exon deletions were observed in many amplified E2 mutant cDNAs. The latter results appear to represent secondary effects on RNA processing that are associated with the MSUD mutations at the E2 locus.
Synthesis and Catalytic Properties of Imidazole-Functionalized Poly(propylene imine)Dendrimers (2002)

The synthesis and characterization of third- and fifth-generation poly(propylene imine) dendrimers terminated with imidazole moieties is reported. Functionalization was achieved using simple peptide coupling reagents. These materials were characte rized by MALDI-MS, NMR, and titration. The use of these endgroup-functionalized dendrimers as catalysts for the hydrolysis of 2,4-dinitrophenyl acetate is described. Molecular simulations provide a basis for interpreting the catalytic data.
Peripheral Blood Neutrophil Granulocytes from Patients with Head and Neck Squamous Cell Carcinoma Functionally Differ from Their Counterparts in Healthy Donors (2011)

Solid tumors such as head and neck squamous cell carcinoma (HNSCC) display an intense interaction between tumoral factors and the immune system. Functional modulation of tumor-infiltrating and peripheral blood immune cells plays an important role during tumor progression. In this pilot study we compared biological functions of polymorphonuclear granulocytes (PMN) from the peripheral blood of HNSCC patients and healthy subjects. PMN were simultaneously isolated from the peripheral blood of HNSCC patients and healthy donors for functional analysis (apoptosis, production of reactive oxygen species (ROS), cytokine release and immunophenotyping). PMN from HNSCC patients showed a significantly lower inducible production of ROS (P = 0.02) and reduced spontaneous apoptosis (P= 0.008) compared with PMN from healthy donors. Under standard culture conditions, there was no significant difference regarding the release of inflammatory cytokines between PMN from HNSCC patients and PMN from healthy donors. Confirming previous observations, serum concentrations of PMN-related cytokines were significantly higher in the peripheral blood of HNSCC patients than in that of controls. Importantly, immunophenotyping revealed an increased number of immature PMN in PMN fractions isolated from HNSCC patients. Peripheral blood PMN from HNSCC patients and healthy donors show distinct functional differences. The presence of increased numbers of immature stages of PMN in HNSCC patients may partly contribute to the changes observed. After recruitment to and infiltration of the tumor, PMN may be further modulated in the local tumor microenvironment. This pilot study justifies functional analyses of myeloid cells in larger cohorts of patients with HNSCC.
논문 : 공간구조론을 적용한 종합병원 외래부 유도사인 배치 및 평가에 관한 연구 -G.D.Weisman의 길찾기 요소를 중심으로 (2015)

Purpose: Signs that are installed at unnoticeable places or that disconnect before the destination can bring errors of location information delivery. Therefore, this study aims to find out the spatial relation between structure of space and signs in the perspective of visual exposure possibility, operating arrangement and assesment by applying spatial structure theory. Methods: Effectiveness of organization of guidance signs was evaluated after the four way-finding factors(Plan Configuration, Sign System, Perceptual Access, Architectural Difference) that G.D.Weisman suggested were interpreted by spatial structure theory(J-Graph analysis, Space Syntax, Visual Graph Analysis) under the premise that it is closely related to the structure of space. Results: 1) Because the south corridor that connects each department of outpatient division is located in the hierarchy center of the space, and walking density is expected to be high, guidance signs need to be organized at the place with high integration value. 2) The depth to the destination space can be estimated through J-Graph analysis. The depth means a switch of direction, and the guidance signs are needed according to the number. 3) According to visibility graph analysis, visual exposure can be different in the same hierarchy unit space according to the shape of the flat surface. Based on these data, location adjustment of signs is possible, and the improvement effect can be estimated quantitatively. Implications: Spatial structure theory can be utilized to design and evaluate sign systems, and it helps to clearly understand the improvement effect. It is desirable to specify design and estimation of sign systems in the order of J-Graph analysis→Space Syntax Theory→ visibility graph analysis.
Recent Advances in LWIR Type-II InAs/GaSb Superlattice Photodetectors and Focal Plane Arrays at the Center for Quantum Devices (2009)

In recent years, Type-II InAs/GaSb superlattice photodetectors have experienced significant improvements in material quality, structural designs, and imaging applications. They now appear to be a possible alternative to the state-of-the-art HgCdTe (MCT) technology in the long (LWIR) and very long wavelength infrared regimes. At the Center for Quantum Devices, we have successfully realized very high quantum efficiency, very high dynamic differential resistance R <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> A-product LWIR Type-II InAs/GaSb superlattice photodiodes with efficient surface passivation techniques. The demonstration of high-quality LWIR focal plane arrays that were 100% fabricated in-house reaffirms the pioneer position of this university-based laboratory.
Essential Oils from Mediterranean Lamiaceae as Weed Germination Inhibitors (2003)

The essential oils obtained from rosemary (Rosmarinus officinalis L.), thyme (Thymus vulgaris L.), and savory (Satureja montana L.) and the four monoterpenes that are their major constituents have been analyzed by GC and GC-MS and tested for their allelopathic properties on the seeds of three different annual weeds (Chenopodium album, Portulaca oleracea, and Echinochloa crus-galli) and three crops (Raphanus sativus, Capsicum annuum, and Lactuca sativa), with the aim to evaluate in vitro their potential as germination inhibitors. The essential oil composition varied with the species, thymol being the main constituent (44%) of thyme and carvacrol (57%) that of savory oil. Differences in essential oil composition were observed within two different rosemary ecotypes, type A, with alpha-pinene (37%) and 1,8-cineole (23%), and type B, characterized by a 2-fold content of 1,8-cineole (47%). This latest essential oil inhibited completely the germination of weeds while concurrently displaying little effect on pepper. The other two oils showed less selective action. S. montana essential oil, with 57% carvacrol, is the most active compound, completely inhibiting germination both of crops and weeds. Borneol, one of the main constituents of the oil of rosemary type B, showed an activity comparable to that of the whole oil. Crop and weed seeds treated with 1,8-cineole showed germination values that were not significantly different from controls, even if a slowing of the germination process expressed in terms of a significant increase in mean germination time was observed. Monoterpene compounds also present in the essential oils mainly represented the volatile fraction released from the crops and their residues into the soil.
Electronic transport studies and photo-detecting properties of indium oxide nanowires (2004)

Single crystalline In/sub 2/O/sub 3/ nanowires were synthesized and then utilized to construct field effect transistors (FET) consisting of individual nanowires. These nanowire transistors exhibited nice n-type semiconductor characteristics with well-defined linear and saturation regimes, and on off ratios as high as 10/sup 4/ were observed at room temperature. The temperature-dependence of the conductance revealed thermal emission as the dominating transport mechanism. Furthermore, photo-conducting properties of In/sub 2/O/sub 3/ nanowires were also studied. These nanowire transistors showed substantial increase in conductance and also significant shifts in the gate threshold voltage upon exposure to ultraviolet (UV) light.
A multi-sample, multi-tree approach to bag-of-words image representation for image retrieval (2009)

The state-of-the-art content based image retrieval systems has been significantly advanced by the introduction of SIFT features and the bag-of-words image representation. Converting an image into a bag-of-words, however, involves three non-trivial steps: feature detection, feature description, and feature quantization. At each of these steps, there is a significant amount of information lost, and the resulted visual words are often not discriminative enough for large scale image retrieval applications. In this paper, we propose a novel multi-sample multi-tree approach to computing the visual word codebook. By encoding more information of the original image feature, our approach generates a much more discriminative visual word codebook that is also efficient in terms of both computation and space consumption, without losing the original repeatability of the visual features. We evaluate our approach using both a ground-truth data set and a real-world large scale image database. Our results show that a significant improvement in both precision and recall can be achieved by using the codebook derived from our approach.
Sensor assisted 3D personal navigation on a smart phone in GPS degraded environments (2011)

Compared to 2D navigation, 3D navigation requires much better performance of locating and heading. In an urban environment, the signals from a built-in GPS on a smart phone are always so weak that it causes considerably low position and heading accuracy. To address this issue and improve the user experience, two contexts, Navigation and Look-Around, are defined in 3D personal navigation. The following methods are applied in this paper: 1) the built-in accelerometer is used for recognizing motion modes of an end-user; 2) a map matching algorithm is implemented for forcing positioning results to be attached on the road; 3) A Bayesian network based heading change detection algorithm is developed by using the built-in digital compass. Finally, the demonstrations in Shanghai World EXPO 2010, China, and Tapiola, Finland are presented.
Mitochondrial Aspartate Aminotransferase Expressed on the Surface of 3T3-L1 Adipocytes Mediates Saturable Fatty Acid Uptake (1995)

Physicochemical studies have suggested that the 43-kDa plasma membrane fatty acid binding protein (FABPpm) is closely related to the mitochondrial isoform of aspartate aminotransferase (mAspAT). In the present studies, mAspAT was not detected immunohistochemically or by immunoblotting in plasma membranes of proliferating 3T3-L1 fibroblasts. During controlled differentiation to an adipocyte phenotype, mAspAT became detectable by the second day of confluent growth, prior to accumulation of visible lipid droplets, and was strongly expressed in 8-day differentiated 3T3-L1 adipocytes. The pattern of expression paralleled the previously reported expression both of FABPpm and of the Vmax for saturable uptake of long chain free fatty acids. As with anti-FABPpm, antibodies to mAspAT selectively inhibited the uptake of [3H]-oleate in 3T3-L1 adipocytes but not in fibroblasts, while having no effect on uptake of either 2-deoxyglucose or the medium chain fatty acid octanoate. Preabsorption of anti-FABPpm with mAspAT, or of anti-mAspAT with FABPpm, abolished immunopositivity in immunohistochemical and immunoblotting studies, as well as the ability of either antibody to inhibit [3H]-oleate uptake. These studies provide strong biologic evidence for the identity of FABPpm and mAspAT, and for the hypothesis that FABPpm/mAspAT mediates the uptake of long chain free fatty acids.