gebhart/patrepeval huggingface dataset:
Purification and Characterization of 210,000-Dalton Inhibitor of Calcium-Activated Neutral Protease from Rabbit Skeletal Muscle and Its Relation to 50,000-Dalton Inhibitor (1985)

An endogenous inhibitor of calcium-activated neutral protease (CANP), which was isolated from rabbit skeletal muscle with chemically drastic pretreatments, comprised major (high-molecular-weight form, HMW-inhibitor) and minor (low-molecular-weight form, LMW-inhibitor) components. HMW-inhibitor was purified to homogeneity using FPLC and preparative electrophoresis. The purified inhibitor appeared as a single protein with a molecular weight of 110,000 on SDS-polyacrylamide gel electrophoresis, and a molecular weight of 210,000 on gel filtration. It was therefore presumed that the inhibitor is a dimer protein under native conditions. It contained large amounts of glutamic acid, alanine, and proline, and small amounts of aromatic amino acids, showing an amino acid composition similar to that of LMW-inhibitor. HMW-inhibitor inhibited CANPs with both low (m-type) and high (mu-type) Ca2+-sensitivity but had no effect on any other proteases examined. It was demonstrated that the inhibition was due to the formation of a stoichiometric complex between rabbit mCANP and inhibitor subunit in the ratio of five to one. These results suggest that HMW-inhibitor might have several reactive sites per molecule and that LMW-inhibitor subunit might be a proteolytic fragment of HMW-inhibitor containing an active site.
Development of an optimized AAV2/5 gene therapy vector for Leber congenital amaurosis owing to defects in RPE65 (2016)

Leber congenital amaurosis is a group of inherited retinal dystrophies that cause severe sight impairment in childhood; RPE65-deficiency causes impaired rod photoreceptor function from birth and progressive impairment of cone photoreceptor function associated with retinal degeneration. In animal models of RPE65 deficiency, subretinal injection of recombinant adeno-associated virus (AAV) 2/2 vectors carrying RPE65 cDNA improves rod photoreceptor function, and intervention at an early stage of disease provides sustained benefit by protecting cone photoreceptors against retinal degeneration. In affected humans, administration of these vectors has resulted to date in relatively modest improvements in photoreceptor function, even when retinal degeneration is comparatively mild, and the duration of benefit is limited by progressive retinal degeneration. We conclude that the demand for RPE65 in humans is not fully met by current vectors, and predict that a more powerful vector will provide more durable benefit. With this aim we have modified the original AAV2/2 vector to generate AAV2/5-OPTIRPE65. The new configuration consists of an AAV vector serotype 5 carrying an optimized hRPE65 promoter and a codon-optimized hRPE65 gene. In mice, AAV2/5-OPTIRPE65 is at least 300-fold more potent than our original AAV2/2 vector.
Enhanced 3D surface related multiple elimination with dual‐sensor data (2011)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 2011Enhanced 3D surface related multiple elimination with dual‐sensor dataAuthors: Rob HeggeTony MartinRoald van BorselenRob HeggePGS, Tony MartinPGS, and Roald van BorselenPGShttps://doi.org/10.1190/1.3627928 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Abstract In recent years dual‐sensor recording has been introduced to marine seismic acquisition with reported benefits such as the increased band‐width of the acquired seismic signal, improved signal‐to‐noise ratios due to deep‐tow streamers, and operational efficiency due to an increased weather window. This abstract describes another advantage of dualsensor measurements, namely the opportunity it provides to better predict and subtract surface‐related multiples from marine seismic data compared to using conventional data acquisition. Using a dataset from offshore Nigeria, it is demonstrated that dual‐sensor acquisition leads to a more accurate set of predicted multiples with respect to phase and amplitudes. Consequently, the adaptive subtraction of the multiples can be carried out in a more constrained and robust manner, leading to better multiple elimination and better primary preservation.Permalink: https://doi.org/10.1190/1.3627928FiguresReferencesRelatedDetailsCited BySimultaneous Long Offset (SLO) towed streamer seismic acquisitionRoald van Borselen, Andrew Long, Eddy von Abendorff, Michael Purves, Justin Norris, and Alcindo Moritz15 January 2014 SEG Technical Program Expanded Abstracts 2011ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 2011 Pages: 4424 Publisher:Society of Exploration Geophysicists HistoryPublished: 08 Aug 2011 CITATION INFORMATION Rob Hegge, Tony Martin, and Roald van Borselen, (2011), "Enhanced 3D surface related multiple elimination with dual‐sensor data," SEG Technical Program Expanded Abstracts : 3510-3514. https://doi.org/10.1190/1.3627928 Plain-Language Summary PDF DownloadLoading ...
Development of a GPS-based Ionospheric TEC Mapping Model for the Australian Region (1996)

IPS Radio and Space Services is developing a real-time ionospheric total electron content (TEC) mapping model based on the Global Positioning System (GPS). This real-time model may be used for monitoring ionospheric conditions and providing the time-delay calibration information for the GPS users in the Australia region. In this paper the GPS-based TEC modelling approach, data processing procedures and a Kalman filtering implementation algorithm are discussed. Preliminary results of a regional ionospheric TEC monitoring network are presented and applications of real-time ionospheric modelling are addressed.
Hacienda Colonial Venezolana: Contadores Mayores e Intendentes de Ejército y Real Hacienda. By Héctor García Chuecos. Publicaciones de la Comisión Preparatoria de a IV Asamblea General del Instituto Panamericano de Geografía e Historia. (Caracas: Editorial Crisol, 1946. Pp. 175.) (1947)

Hacienda Colonial Venezolana: Contadores Mayores e Intendentes de Ejército y Real Hacienda. By Héctor García Chuecos. Publicaciones de la Comisión Preparatoria de a IV Asamblea General del Instituto Panamericano de Geografía e Historia. (Caracas: Editorial Crisol, 1946. Pp. 175.) - Volume 3 Issue 4
Safeguarding Stem Cell-Based Regenerative Therapy against Iatrogenic Cancerogenesis: Transgenic Expression of DNASE1, DNASE1L3, DNASE2,DFFB Controlled By POLA1 Promoter in Proliferating and Directed Differentiation Resisting Human (2013)

Herein, we describe attaining the proof-of-concept for the strategy, whereby transgenic expression of the genetically engineered human recombinant DNases in proliferating and directed differentiation resisting stem cells leads to their death. This novel strategy reduces the risk of iatrogenic neoplasms in stem cell therapy.
310-MHz SAW resonator with <i>Q</i> at the material limit (1977)

Two-port SAW resonators have been made on ST-cut quartz to operate at 310 MHz with unloaded Q values in vacuum in excess of 32 000 (the limit on Q imposed by intrinsic material loss at this frequency is approximately 33 000). Design principles and fabrication techniques employed in the realization of these devices are described.
Location Based Beamforming in 5G Ultra-Dense Networks (2016)

In this paper we consider transmit (Tx) and receive (Rx) beamforming schemes based on the location of the device. In particular, we propose a design methodology for the Tx/Rx beamforming weight-vectors that is based on the departure and arrival angles of the line-of-sight (LoS) path between access-nodes (ANds) and user-nodes (UNds). A network-centric extended Kalman filter (EKF) is also proposed for estimating and tracking the directional parameters needed for designing the Tx and Rx beamforming weights. The proposed approach is particularly useful in 5G ultra-dense networks (UDNs) since the high-probability of LoS condition makes it possible to design geometric beams at both Tx and Rx in order to increase the signal-to-interference-plus-noise ratio (SINR). Moreover, relying on the location of the UNd relative to the ANds makes it possible to replace fullband uplink (UL) reference signals, commonly employed for acquiring the channel-state-information-at-transmitter (CSIT) in time-division-duplex (TDD) systems, by narrowband UL pilots. Also, employing the EKF for tracking the double-directional parameters of the LoS-path allows one to reduce the rate at which UL reference signals are transmitted. Consequently, savings in terms of time-frequency resources are achieved compared to beamforming schemes based on full-band CSI. Extensive numerical results are included using a realistic ray-tracing based system-level simulator in ultra-dense 5G network context. Results show that position based beamforming schemes outperform those based on full-band CSI in terms of mean user-throughput even for highly mobile users.
Predicting synergism in binary mixtures of surfactants (2007)

Chemicals in the environment continue to cause concern. One method of decreasing the environmental impact of surfactants is to design new molecules that are more efficient and effective in their interfacial activity. New types of surfactants with two hydrophilic and two or three hydrophobic groups in the molecule, called gemini surfactants, exhibit these characteristics. When properly designed, they can be as much as three orders of magnitude more efficient in reducing the surface tension of aqueous solutions than comparable conventional surfactants. A second method is to use mixtures of known surfactants that exhibit synergism in their interfacial properties. This paper will discuss the second method.The requirements, in quantitative terms, for the existence of synergy in mixtures of surfactants have been elucidated for several interfacial phenomena. They involve an interaction (β) parameter whose various values, for mixed monolayer formation at different interfaces and for mixed micelle formation in the solution phase, can be determined from surface, interfacial, or adhesion tension data on the individual interface-active components and at least one mixture of them. This, together with relevant data on the individual components, permits one to predict not only whether the mixture will exhibit synergy, but also the ratio of the two surfactants needed to reach the point of maximum synergism and the value of the investigated property at that point. Since synergy depends so greatly on the value of the β parameter, this discussion will also cover the effect on the β value of the chemical structures of the interface-active components of the mixture, the nature of the interface, and the molecular environment.
Just Say No to ATOH: How <i>HIC1</i> Methylation Might Predispose Medulloblastoma to Lineage Addiction (2008)

Hypermethylated in cancer-1 (HIC1) is a tumor suppressor frequently targeted for promoter hypermethylation in medulloblastoma, an embryonal tumor of the cerebellum. Recently, we showed that HIC1 is a direct transcriptional repressor of ATOH1, a proneural transcription factor required for normal cerebellar development, as well as for medulloblastoma cell viability. Because demethylating agents can induce reexpression of silenced tumor suppressors, restoring HIC1 function may present an attractive therapeutic avenue in medulloblastoma by exploiting an apparent addiction to ATOH1.
Growth and Resistivity Characteristics of Undoped Semi-Insulating GaAs Crystals with Low Dislocation Density (1984)

Remarkably low dislocation density of approximately 1000/cm 2 (minimum 600/cm 2 ) and uniform distribution were attained in two inch diameter undoped GaAs single crystals grown by an improved LEC technique. Resistivity was revealed to be high enough (1×10 8 Ω·cm) and homogeneity was remarkably improved over that of conventional high dislocation density LEC crystal.
Role of antioxidant property of carvedilol in mild to moderate hypertensive patients: A prospective open-label study (2016)

<b>Objective:</b> Carvedilol is a nonselective third generation β-blocker that does not display the negative effects of traditional β-blockers. Regarding the antioxidant, anti-inflammatory and distinct metabolic properties of carvedilol which are similar to that of high-density lipoprotein (HDL) and paraoxonase 1 (PON1), the present study intends to investigate the effects of carvedilol treatment on malondialdehyde (MDA) and soluble lectin-like ox-low-density lipoprotein (LDL) receptor (sLOX-1) as markers of oxidative stress in association to lipid profiles, apolipoproteins (apo), and PON1 activity in hypertensive patients.<br><b>Patients and Methods:</b> This clinical trial study was performed on forty patients with mild to moderate essential hypertension. Subjects were studied before and after 2 months treatment with carvedilol, 25 mg daily. Lipids and lipoproteins were measured using a biochemistry analyzer. PON and arylesterase activity were assayed using paraoxon and phenyl acetate as substrates, respectively. MDA was quantified using a chemical colorimetric assay. ELISA was used to measure sLOX-1.<br><b>Results:</b> Our results showed that carvedilol treatment decreased systolic and diastolic blood pressure as much as forty and 16 mmHg, respectively (<i>P</i> < 0.001). It also increased HDL, total cholesterol, and serum PON1 activity (<i>P</i> < 0.05), but the levels of triglyceride, LDL, apo A-I, and apo B did not significantly change. There was an inverse correlation between serum PON1 activity and serum MDA.<br><b>Conclusion:</b> This study confirmed the antihypertensive effect of the drug and its beneficial metabolic effects through augmenting HDL and PON1 activity. We propose that the antioxidant effects of carvedilol can be partially attributed to increased PON-1 activity.
Varus–valgus alignment: Reduced risk of subsequent cartilage loss in the less loaded compartment (2011)

Neutral and valgus alignment were each associated with a reduction in the risk of subsequent cartilage loss in certain medial subregions and neutral and varus alignment with a reduction in the risk of cartilage loss in certain lateral subregions. These results support load redistribution as an in vivo mechanism of the long-term alignment effects on cartilage loss in knee OA.
Characterization of <i>Staphylococcus aureus</i> Isolates with a Partial or Complete Absence of Staphylococcal Cassette Chromosome Elements (2010)

Detection of methicillin-resistant Staphylococcus aureus (MRSA) by single-locus PCR assays that target the extremity of the staphylococcal cassette chromosome-mec (SCCmec) and part of the adjacent S. aureus-specific open reading frame gene (orfX) is a significant diagnostic advancement, since it provides real-time detection directly from screening specimens. However, isolates harboring mecA deletions within SCCmec may result in false-positive identification of MRSA in these assays. We characterized 24 methicillin-susceptible S. aureus (MSSA) isolates that tested positive in one such assay to investigate this phenomenon. Seven isolates resembled USA100 and carried SCCmec II elements with mecA deletions that spanned 20 to 46 kbp. The mecA excisions in USA100-resembling isolates appeared to be linked with IS431 transposable elements present in SCCmec II. For 17 isolates that resembled USA400 and/or MSSA476, the identity and possible excision of SCC elements could not be confirmed. The downstream common sequence (dcs) shared by SCCmec I, II, and IV elements was detected in these isolates. Sequence analysis of the chromosomal regions flanking the missing SCC element revealed an intact SCC integration site, a duplicate dcs, and the enterotoxin gene cluster downstream of orfX. An annealing sequence for one of the SCCmec-specific primers (mecii574) in the single-locus PCR assay was identified in the duplicate dcs. In the absence of SCC, a 176-bp amplicon can be generated from this mecii574 annealing sequence to yield a false-positive result. In conclusion, partial SCCmec II excisions via IS431 elements in strains that resembled USA100 and the presence of a duplicate mecii574 annealing sequence in strains that resembled USA400/MSSA476 were identified as causes for false-positive results in a single-locus PCR assay that targets the SCCmec/orfX junction.
Quad/Triangle Subdivision (2003)

Abstract In this paper we introduce a new subdivision operator that unifies triangular and quadrilateral subdivision schemes. Designers often want the added flexibility of having both quads and triangles in their models. It is also well known that triangle meshes generate poor limit surfaces when using a quad scheme, while quad‐only meshes behave poorly with triangular schemes. Our new scheme is a generalization of the well known Catmull‐Clark and Loop subdivision algorithms. We show that our surfaces are C 1 everywhere and provide a proof that it is impossible to construct such a C 2 scheme at the quad/triangle boundary. However, we provide rules that produce surfaces with bounded curvature at the regular quad/triangle boundary and provide optimal masks that minimize the curvature divergence elsewhere. We demonstrate the visual quality of our surfaces with several examples. ACM CSS: I.3.5 Computer Graphics— Curve, surface, solid, and object representations
Self-Assembled Au/TiO<SUB>2</SUB>/CNTs Ternary Nanocomposites for Photocatalytic Applications (2010)

10.1166/sam.2010.1116
Separation of monoglycerides, diglycerides, and triglycerides by liquid‐liquid extraction (1956)

Summary Ternary‐equilibrium data were obtained at 60°C. for mono‐ and dilaurin, and di‐ and trilaurin with aqueous alcohols. Distribution between phases and solvent selectivity are shown graphically. Variation of solvent polarity by water addition caused a shift in the phase boundary curve so that either Type 1 or Type 2 diagrams could be obtained. Pure mono‐, di‐, and trilaurin mixtures, and commercial monoglycerides were studied as quaternary systems for monoglyceride distribution with 50 wt.‐% aqueous ethanol. Pure and commercial systems showed similar distribution tendencies even with differences in molecular weight and degree of unsaturation. Solvent selectivity data (β) for quaternary systems were predicted from ternary systems studied with a common solvent. Aqueous ethanol will separate mixtures of mono‐, di‐, and triglycerides by selective liquid‐liquid extraction; however the literature favors more complex systems based on two immiscible solvents. Continuous countercurrent extraction of commercial monoglycerides by 40% aqueous ethanol solution in a four‐stage agitated column gave a 15% over‐all stage efficiency. Cooling the extract to 0°C. precipitated 96.6% of the extract solids. Flow diagrams were prepared for batch and continuous extraction processes.
Isolation characteristics for laser diode optical switches (1984)

The isolation spectra for laser diode optical switches, which are the level difference between the on and off state attenuation, are calculated for n- and p-type GaAlAs and InGaAsP using a Gaussian fit to the Halperin–Lax band tails and Stern’s matrix element model. The measured and calculated isolation spectra for a GaAlAs laser diode are closely coincident with one another. The calculated results show that p-type materials, which contain small background carrier concentrations, are suitable for high isolation laser diode optical switches. Especially, it becomes clear that p-type In0.533Ga0.467As laser diode optical switches, whose operating wavelength is 1.65 μm, give the best isolation characteristics.
Copy protection for automotive electronic control units using authenticity heartbeat signals (2012)

Protection of intellectual property rights is a vital aspect for the future automotive supplier market, in particular for the aftersales market for ECUs. Computer security can deliver the required protection mechanisms and sustain the according business models. We propose an approach to facilitate the rigorous checking of components for originality in a vehicle. In our system model, a security controller receives special messages (i.e., the authenticity heartbeat signal) from relevant ECUs and it performs subsequent authentication and plausibility checks. As a result, the security controller can tell, if the current setup of components in the vehicle is original. We evaluate our authentication architecture for the Battery Management System (BMS) of a hybrid car. Here, the security controller detects reliably, if the BMS is an original component, and whether an attacker has modified the operational limits of the battery. In this paper, we reason that an effective copy protection scheme needs to fuse relevant information from different sources. Therefore, various security techniques have to be combined in a sound architectural approach. The distinctive feature of our architecture is that it takes into account application-specific knowledge of the real-time entities under control.
GaN-FET based dual active bridge DC-DC converter (2011)

This paper describes a high step-down unregulated, fixed-ratio DC-DC converter (DCX) based on the dual active bridge (DAB) power stage operating at high switching frequency using enhancement-mode Gallium-Nitride-on-Silicon (GaN) transistors. The DAB power stage design as well as a comparison of losses using GaN and silicon MOS devices is based on a detailed state-plane analysis of resonant transitions. Experimental results are presented for a 150 W, 150-to-12 V prototype DCX operating at 1 MHz switching frequency.
Combining CSAMT and MT in difficult exploration environments (1997)

The application of electromagnetic tools has become an important part of exploration for natural resources, particularly in environments (such as areas with volcanic or carbonate cover) where collection of seismic data is difficult. A commonly applied alternative to seismic, particularly when searching for hydrocarbons or deep geothermal resources, is the natural source magnetotelluric (MT) method. Since this method’s depth of investigation is independent of the set-up geometry, soundings can be made to several kilometers. This is significantly more than logistically reasonable sounding limits when using time-domain or DC electrical methods.