gebhart/patrepeval huggingface dataset:
Peritoneal inflammation – A microenvironment for Epithelial Ovarian Cancer (EOC) (2004)

Epithelial ovarian cancer (EOC) is a significant cause of cancer related morbidity and mortality in women. Preferential involvement of peritoneal structures contributes to the overall poor outcome in EOC patients. Advances in biotechnology, such as cDNA microarray, are a product of the Human Genome Project and are beginning to provide fresh opportunities to understand the biology of EOC. In particular, it is now possible to examine in depth, at the molecular level, the complex relationship between the tumor itself and its surrounding microenvironment.This review focuses on the anatomy, physiology, and current immunobiologic research of peritoneal structures, and addresses certain potentially useful animal models. Changes in both the inflammatory and non-inflammatory cell compartments, as well as alterations to the extracellular matrix, appear to be signal events that contribute to the remodeling effects of the peritoneal stroma and surface epithelial cells on tumor growth and spread. These alterations may involve a number of proteins, including cytokines, chemokines, growth factors, either membrane or non-membrane bound, and integrins. Interactions between these molecules and molecular structures within the extracellular matrix, such as collagens and the proteoglycans, may contribute to a peritoneal mesothelial surface and stromal environment that is conducive to tumor cell proliferation and invasion. These alterations need to be examined and defined as possible prosnosticators and as therapeutic or diagnostic targets.
Correction of chromatic and spherical aberration in electron microscopy utilizing the time structure of pulsed excitation sources (2002)

A theoretical ansatz for correction of the chromatic and spherical aberration of round-lens systems in photoemission and low-energy electron microscopy (PEEM/LEEM) is presented. The method is based on fast switching of electrical acceleration or lens fields. It exploits the highly precise time structure of pulsed photon sources like electron storage rings for synchrotron radiation or pulsed lasers as well as pulsed photocathodes of a LEEM. The initial results indicate that the approach is a promising alternative to the implementation of multipole or mirror correctors in the electron optical column of a microscope.
Augmented autologous transfusions in major reconstructive spine surgery (1998)

Intraoperative autologous transfusion during major reconstructive spine surgery decreased allogeneic red blood cell transfusions, but patients were exposed to significant numbers of allogeneic blood products. This study reports a prospective study of 160 randomized patients undergoing major reconstructive spine surgery. Without delaying start of surgery, 80 patients underwent hemapheresis with coincidental normovolemic hemodilution in the operating room to sequester autologous blood components. A therapeutic dose plateletpheresis product and an average of 2 U each of freshly collected autologous red cells and fresh plasma were prepared prior to surgical incision. The same supplies and equipment were used subsequently to carry out intraoperative autologous transfusion (IAT). Autologous plasma and platelets were transfused to Sequestration patients, contributing to a significant decrease of total allogeneic donor exposures. One or more autologous red blood cell unit equivalents were cost effectively salvaged and retransfused to 78% of the Sequestration patients. Altogether, autologous red cells comprised 87% of the total red cells transfused. During the same time period, 80 age-, sex-, and weight-matched controls, who received IAT only, were accrued for comparison with Sequestration patients. Of all red cells transfused to control patients, autologous units comprised 47%. Both patient groups received the same total perioperative red blood cell support. The per patient cost for IAT, with or without sequestration, was competitive with supplying one unit of cross-matched allogeneic red cells. IAT only patients had greater allogeneic blood donor exposures than Sequestration patients, in whom the numbers of allogeneic red cells, plasma and platelet transfusions were decreased. Compared with IAT alone, the hospital post-operative stay of Sequestration patients was 23% less than IAT only patients.
Phosphorylated serine 28 of histone H3 is associated with destabilized nucleosomes in transcribed chromatin (2007)

Histone modifications and variants have key roles in the activation and silencing of genes. Phosphorylation of histone H3 at serine 10 and serine 28 is involved in transcriptional activation of genes responding to stress or mitogen-stimulated signaling pathways. The distribution of H3-modified isoforms in G0 phase chicken erythrocyte chromatin was investigated. H3 phosphorylated at serine 28 was found highly enriched in the active/competent gene fractions, as was H3 di- and trimethylated at lysine 4. The H3 variant H3.3 in this chromatin fraction was preferentially phosphorylated at serine 28. Conversely, H3 phosphorylated at serine 10 was present in all chromatin fractions, while H3 dimethylated at lysine 9 was associated with the chromatin-containing repressed genes. H3 phosphorylated at serine 28 was located at the promoter region of the transcriptionally active, but not competent, histone H5 and beta-globin genes. We provide evidence that H3.3 phosphorylated at serine 28 was present in labile nucleosomes. We propose that destabilized nucleosomes containing H3.3 phosphorylated at serine 28 aid in the dynamic disassembly-assembly of nucleosomes in active promoters.
Building an Effective Representation for Dynamic Networks (2006)

A dynamic network is a special type of network composed of connected transactors which have repeated evolving interaction. Data on large dynamic networks such as telecommunications networks and the Internet are pervasive. However, representing dynamic networks in a manner that is conducive to efficient large-scale analysis is a challenge. In this article, we represent dynamic graphs using a data structure introduced in an earlier article. We advocate their representation because it accounts for the evolution of relationships between transactors through time, mitigates noise at the local transactor level, and allows for the removal of stale relationships. Our work improves on their heuristic arguments by formalizing the representation with three tunable parameters. In doing this, we develop a generic framework for evaluating and tuning any dynamic graph. We show that the storage saving approximations involved in the representation do not affect predictive performance, and typically improve it. We motivate our approach using a fraud detection example from the telecommunications industry, and demonstrate that we can outperform published results on the fraud detection task. In addition, we present a preliminary analysis on Web logs and e-mail networks.
Delays in Construction of Nuclear Power Plants (1979)

Fifteen separate interviews were conducted with 49 design and construction staff personnel representing five organizations. The results indicated that the most important items in the design-construction cycle of a nuclear power plant are overall coordination, equipment availability, design-construct lead-time, and handling of design changes. Coordination encompasses activities necessary to handle all the other high impact delay cases. Use of computerized systems facilitates storing, handling, and sorting of the data for the many different tasks of the coordinating teams. The southeastern utilities have taken major steps in development and implementation of management information systems and innovative project control systems. The Tennessee Valley Authority and Duke Power Company have made impressive progress in objective identification and system implementation. Other companies have requested their design-construct contractors to develop and use computer-based systems for scheduling, procurement, and startup testing.
Mammalian <i>EGLN</i> genes have distinct patterns of mRNA expression and regulation (2002)

The mammalian EGLN family contains three paralagous genes (EGLN1, EGLN2, and EGLN3) encoding prolyl hydroxylase isoforms that mediate the oxygen-dependent targeting of the transcription factor hypoxia inducible factor alpha to the proteosome. The rat orthologue of EGLN3 (SM-20) exhibits tissue-restricted expression, is induced by growth factors in cultured vascular smooth muscle, and is up-regulated during myogenesis. To determine if all three EGLN genes are coordinately regulated, we examined their mRNA expression in murine tissues and in cultured cells. We now report that the three murine EGLN mRNAs have unique but overlapping patterns of tissue expression. The most striking differences were in the heart, where EGLN3 had its highest levels of expression, and the testis, where EGLN2 was the only isoform expressed. In cultured vascular smooth muscle cells, serum treatment led to up-regulation of EGLN1 and EGLN3, but not EGLN2, and only EGLN3 was superinduced by cyclohexamide. In cultured C2C12 myocytes, EGLN3 was up-regulated during differentiation, whereas EGLN1 and EGLN2 were constitutively expressed. The abundance of EGLN3 mRNA in the heart, its induction by growth factors in vascular smooth muscle, and its regulation during C2C12 differentiation suggest a unique role for EGLN3 and might justify the development of isoformspecific inhibitors.
Co-design of interleaved memory systems (2000)

Memory interleaving is a cost-efficient approach to increase bandwidth. Improving data access locality and reducing memory access conflicts are two important aspects to achieve high efficiency for interleaved memory. In this paper, we introduce a design framework that integrates these two optimizations, in order to find out minimal memory banks and channels required in the embedded system under performance restriction. Several important techniques, loop and data layout transformations for data access locality, extracting data streams, conflict cache miss reduction as well as data placement and optimally reordered access for interleaved memories, are incorporated in the design framework. Experiments show that our co-design method results in substantially less hardware requirement compared to the implementations without optimization.
Comparison of the Stem-Loop and Linear Probe-Based Electrochemical DNA Sensors by Alternating Current Voltammetry and Cyclic Voltammetry (2011)

Here we systematically characterized the sensor performance of the stem-loop probe (SLP) and linear probe (LP) electrochemical DNA sensors using alternating current voltammetry (ACV) and cyclic voltammetry (CV), with the goal of generating the set of operational criteria that best suits each sensor architecture, in addition to elucidating the signaling mechanism behind these sensors. Although the LP sensor shows slightly better % signal suppression (SS) upon hybridization with the perfect match target at 10 Hz, our frequency-dependent study suggests that it shows optimal % SS only in a very limited AC frequency range. Similar results are observed in CV studies in which the LP sensor, when compared to the SLP sensor, displays a narrower range of voltammetric scan rates where the optimal % SS can be achieved. More importantly, the difference between the two sensors' performance is particularly pronounced if the change in integrated charge (Q) upon target hybridization, rather than the peak current (I), is measured in CV. The temperature-dependent study further highlights the differences between the two sensors, where the LP sensor, owing to the flexible linear probe architecture, is more readily perturbed by temperature changes. Both SLP and LP sensors, however, show a loss of % SS when operated at elevated temperatures, despite the significant improvement in the hybridization kinetics. In conjunction with the ACV, CV, and temperature-dependent studies, the electron-transfer kinetics study provides further evidence in support of the proposed signaling mechanism of these two sensors, in which the SLP sensor's signaling efficiency and sensor performance is directly linked to the hybridization-induced conformational change in the redox-labeled probe, whereas the performance of the LP sensor relies on the hybridization-induced change in probe dynamics.
DIETHYLBARBITURATE BUFFER (1930)

In the series of buffers now used, there is no other buffer system to cover the range around pH = 8 with a sufficient reliability except for the borate buffer of Sorensen.This buffer has, however, certain disadvantages which are strongly felt in practical use.The borate buffer Eannot be used in compounds containing two or more hydroxyl groups, such as polyphenols, polyhydric alcohols, and especially carbohydrates of any kind.These substances combine with boric acid to form complex acids of an appreciable strength.Not only is the pH of the borate buffer perceptibly shifted in the presence of those substances but, what is worse, those substances themselves are no longer present in their original state in contact with the borate buffer.This disadvantage was encountered on the occasion of the investigation of the effect of pH on the activity of all carbohydrate-splitting enzymes and on the measurement of oxidation-reduction potentials of certain dyestuffs containing two hydroxyl groups, to be described in a subsequent paper.This gap in the buffer series is due to the fact that practically no acid was known with a dissociation constant around lo-*.Recently Kolthoff (1) found a dissociation constant of approximately lo+ for diethyl,barbituric acid (veronal).So it seemed likely to me that this acid might be useful as a buffer for the wanted range.This turned out to be true.The following data, calculated from the experimental calibration with the hydrogen electrode, will suffice for practical purposes.10.30 gm. of sodium diethylbarbiturate (Verona1 sodium) are dissolved in COz-free water to a volume of 500 cc.The quality of the commercial preparation was satisfactory without recrystalliza-33
High Stem Cell Frequency in Acute Myeloid Leukemia at Diagnosis Predicts High Minimal Residual Disease and Poor Survival (2005)

Both in vivo data, as well as the correlation studies, show that AML stem cell frequency at diagnosis offers a new prognostic factor. From our data, it is tempting to hypothesize that a large CD34(+)CD38(-) population at diagnosis reflects a higher percentage of chemotherapy-resistant cells that will lead to the outgrowth of MRD, thereby affecting clinical outcome. Ultimately, future therapies should be directed toward malignant stem cells.
The study of diffusion weighted imaging and MR spectroscopy in auditory cortex and related area of prelingual hearing-loss patients (2008)

Objective To study the time-dependent changes and mechanisms of auditory cortex in prelingual sensorineural hearing loss. Methods Two groups (9-12, 19-22-year-old) of hearing-loss patients and matched normal hearing subjects received MR diffusion and MRS examination. The ROI for ADC measurement were placed on the Heschl gyrus (HG), and for NAA/Cr, Cho/Cr on the superior temporal gyrus(STG). Results There was no statistical difference among groups both in the NAA/Cr and Cho/Cr (P>0.05), Left side NAA/Cr 2.46±0.51, 2.49±0.52, 2.26±0.33;Right side NAA/Cr 2.26±0. 46, 2. 44±0. 45, 2. 27±0. 46. Left side Cho/Cr 0. 88±0. 21,0. 92±0. 18, 0. 87±0. 13 ; Right side Cho/Cr 0. 88 ± 0. 18, 0. 87 ± 0. 22, 0. 81 ± 0. 16. There was statistical difference of ADC value among normal hearing subjects and 9-12, 19-22-year-old hearing-loss patients(F = 4. 42, P /s, Right side (848 ± 73),(880 ± 61), (902 ± 52) mm2/s. Conclusion Compared with the normal hearing, the ADC value increased in 20-year-length of deafness. The NAA/Cr and Cho/Cr of STG showed no changes among the three groups.

Key words: 
Hearing loss, sonsorineural;  Magnetic resonance imaging, diffusion;  Magneticresonance spectroscopy
Genome‐wide analysis of acute myeloid leukemia with normal karyotype reveals a unique pattern of homeobox gene expression distinct from those with translocation‐mediated fusion events (2003)

Gene expression profiles were determined from presentation peripheral blood and bone marrow samples of 28 patients with acute myeloid leukemia (AML). Hierarchical clustering sorted the profiles into separate groups, each representing one of the major cytogenetic classes in AML [i.e., t(8;21), t(15;17), inv(16), 11q23, and normal karyotype]. Statistical group comparison identified genes whose expression was strongly correlated with these chromosomal classes. Moreover, the normal karyotype AMLs were characterized by distinctive up-regulation of certain members of the class I homeobox A and B gene families, implying a common underlying genetic lesion. These data reveal novel diagnostic and therapeutic targets and demonstrate the potential of microarray-based dissection of AML.
Changes in hsp70 alter thermotolerance and heat-shock regulation in Drosophila. (1991)

To test the role of the heat shock protein hsp70 in induced thermotolerance and in the regulation of the heat-shock response, we established cell lines with altered expression of the Hsp70 gene. Underexpressing cells were created by transformation with antisense Hsp70 genes, and overexpressing cells by transformation with extra copies of the wild-type gene. Expression at normal temperatures was achieved by placing Hsp70 coding sequences under the control of the metallothionein promoter. Cells that expressed mutant hsp70s were created by transforming cells with deletion and frameshift mutations. The results indicate that hsp70 plays a major role in both thermotolerance and regulation. Surprisingly, they also indicate that these functions can be separated. Overexpression affected thermotolerance more than regulation; underexpression affected regulation more than thermotolerance. A carboxyl-terminal deletion of Hsp70 had a severe dominant-negative effect on thermotolerance but only a minor effect on regulation; an amino-terminal deletion strongly affected regulation but not thermotolerance. A model that explains these observations is presented.
A Multiple-Wire Proportional Counter for Fast Neutron Detection (1954)

A cylindrical chamber, 8.5 in. in length and 8.5 in. in diameter, containing two sets of parallel multiple-wire grids has been built and operated as a proportional counter for fast neutron detection. The collecting wires are 0.001 in. in diameter. Filled with pure CH4 at about atmospheric pressure and operated at 3400 v, it serves to detect neutrons up to 10 Mev, with an average efficiency of 0.17 percent. The energy distribution of neutrons from 0.3 to 3 Mev can be estimated. The multiple-wire counter is completely insensitive to gamma radiation under these operating conditions. This instrument has been used to make a survey of fast neutrons around the U. C. L. A. cyclotron. At higher pressures the instrument can presumably be used to measure neutron spectra of higher energy.
Biotransformation of linoleic acid and bile acids by Eubacterium lentum (1984)

Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. Mahony, and L. V. Holdeman, Appl. Environ. Microbiol. 37:992-1000, 1979). All strains alkalized (pH 8 to 8.5) arginine-containing (2 to 15 mg/ml) culture media, and growth of the majority of the strains was stimulated by arginine. All strains converted linoleic acid into transvaccenic acid by shifting the 12,13-cis double bond of linoleic acid into an 11,12-trans(?) double bond followed by biohydrogenation of the 9,10-cis double bond. Hence, biohydrogenation of linoleic acid is a new general characteristic of E. lentum. The 33 strains were also studied for bile acid deconjugase and hydroxysteroid dehydrogenase (HSDH) activities. The 6 strains in group D were steroid inactive; the 27 strains in groups A, B, C, and E were steroid active. The steroid-active group contained bile acid deconjugase-producing strains (groups C and E, plus strain 116 in group A) and nondeconjugating strains. All nondeconjugating strains of groups A and B developed 7 alpha- and 12 alpha-HSDH activities and contained 3 alpha-HSDH-positive strains and 3 alpha-HSDH-negative strains. Deconjugating strains varied in HSDH activities.
Structure of Ni(II) and Ru(III) Ammine Complexes Grafted onto Mesoporous Silicate Sieve (2003)

Ru(III) and Ni(II) ammine complexes were grafted onto the pore surface of MCM-41 by ion exchange from basic aqueous solution. Covalent anchoring of the type M−O−Si (M = Ru or Ni) is obtained by this method. The structure of the grafted complexes was studied by UV−vis, FT-IR, and X-ray absorption spectroscopy. [Ru(NH3)5Cl]2+ readily yielded monopodal grafting, while dehydration was needed in the case of [Ru(NH3)6]3+ to achieve substitution of a NH3 ligand with a siloxy group. The interaction of charge-compensating siloxy groups with remaining NH3 ligands further stabilized the grafted Ru(III) centers. Dipodal grafting of Ni(II) was obtained with [Ni(NH3)6-x(H2O)x]2+ (x = 1 or 2). Upon dehydration, the dipodally grafted Ni(II) complexes are converted in a reversible process to a tripodally anchored form through substitution of a H2O ligand by a silanol group. The formation of layered Ni silicate structures, a dominant product in the Ni grafted amorphous silica, was not observed. This demonstrates that simple coordination complexes can be used for accomplishing covalent attachment of isolated metal centers on a mesoporous silicate sieve in high yield. Grafting of Ni(II) onto MCM-41 containing isolated Ti(IV) centers resulted in the formation Ti(IV)−O−Ni(II) moieties, which are of special interest for photochemical applications.
Elevated Local and Serum CX3CL1(Fractalkine) Expression and Its Association with Disease Severity in Patients with Psoriasis. (2015)

These results suggest that FKN may play an important role in the pathogenesis of psoriasis and represent a reliable biomarker to reflect disease severity. Therapeutic interventions that target FKN in psoriasis deserve further study.
Effect of Electromagnetic Field Exposure on Chemically Induced Differentiation of Friend Erythroleukemia Cells (2000)

Whether exposure of humans to extremely low frequency electromagnetic fields (ELF-EMF) can cause cancer is controversial and therefore needs further research. We used a Friend erythroleukemia cell line that can be chemically induced to differentiate to determine whether ELF-EMF could alter proliferation and differentiation in these cells in a manner similar to that of a chemical tumor promoter. Exposure of this cell line to 60 Hz ELF-EMF resulted in a dose dependent inhibition of differentiation, with maximal inhibition peaking at 40% and 40 mG (4 microT). ELF-EMF at 10 mG (1.0 microT) and 25 mG (2.5 microT) inhibited differentiation at 0 and 20%, respectively. ELF-EMF at 1.0 (100) and 10.0 G (1,000 microT) stimulated cell proliferation 50% above the sham-treated cells. The activity of telomerase, a marker of undifferentiated cells, decreased 100[times] when the cells were induced to differentiate under sham conditions, but when the cells were exposed to 0.5 G (50 microT) there was only a 10[times] decrease. In summary, ELF-EMF can partially block the differentiation of Friend erythroleukemia cells, and this results in a larger population of cells remaining in the undifferentiated, proliferative state, which is similar to the published results of Friend erythroleukemia cells treated with chemical-tumor promoters.