gebhart/patrepeval huggingface dataset:
Reduction of Hox Gene Expression by Histone H1 Depletion (2012)

The evolutionarily conserved homeotic (Hox) genes are organized in clusters and expressed collinearly to specify body patterning during embryonic development. Chromatin reorganization and decompaction are intimately connected with Hox gene activation. Linker histone H1 plays a key role in facilitating folding of higher order chromatin structure. Previous studies have shown that deletion of three somatic H1 subtypes together leads to embryonic lethality and that H1c/H1d/H1e triple knockout (TKO) embryonic stem cells (ESCs) display bulk chromatin decompaction. To investigate the potential role of H1 and higher order chromatin folding in the regulation of Hox gene expression, we systematically analyzed the expression of all 39 Hox genes in triple H1 null mouse embryos and ESCs by quantitative RT-PCR. Surprisingly, we find that H1 depletion causes significant reduction in the expression of a broad range of Hox genes in embryos and ESCs. To examine if any of the three H1 subtypes (H1c, H1d and H1e) is responsible for decreased expression of Hox gene in triple-H1 null ESCs, we derived and characterized H1c(-/-), H1d(-/-), and H1e(-/-) single-H1 null ESCs. We show that deletion of individual H1 subtypes results in down-regulation of specific Hox genes in ESCs. Finally we demonstrate that, in triple-H1- and single-H1-null ESCs, the levels of H3K4 trimethylation (H3K4me3) and H3K27 trimethylation (H3K27me3) were affected at specific Hox genes with decreased expression. Our data demonstrate that marked reduction in total H1 levels causes significant reduction in both expression and the level of active histone mark H3K4me3 at many Hox genes and that individual H1 subtypes may also contribute to the regulation of specific Hox gene expression. We suggest possible mechanisms for such an unexpected role of histone H1 in Hox gene regulation.
Pyrimidine-Core Extended π-Systems:  General Synthesis and Interesting Fluorescent Properties (2004)

We have developed a simple but powerful synthetic strategy that permits the assembly of pi-systems onto a pyrimidine core in a programmable and diversity-oriented format. The nucleophilic addition of ArLi to 2-methylthiopyrimidine, followed by 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) oxidation, resulted in the production of 4-aryl-2-methylthiopyrimidines. The iterative reaction sequence then gave 4,6-diaryl-2-methylthiopyrimidines. The resulting adduct was further allowed to react with ArMgBr under the catalytic influence of NiCl2(dppe) to afford 2,4,6-triarylpyrimidines. By following this synthetic scheme, interesting pyrimidine-core pi-systems were rapidly constructed in a programmable fashion. The successful discovery of a number of interesting fluorescent materials and properties (e.g., solvatofluorochromism) speaks well for the potential of our platform strategy in the development of functional organic materials.
Rational Optimization of Drug-Target Residence Time: Insights from Inhibitor Binding to the <i>Staphylococcus aureus</i> FabI Enzyme–Product Complex (2013)

Drug-target kinetics has recently emerged as an especially important facet of the drug discovery process. In particular, prolonged drug-target residence times may confer enhanced efficacy and selectivity in the open in vivo system. However, the lack of accurate kinetic and structural data for a series of congeneric compounds hinders the rational design of inhibitors with decreased off-rates. Therefore, we chose the Staphylococcus aureus enoyl-ACP reductase (saFabI)--an important target for the development of new anti-staphylococcal drugs--as a model system to rationalize and optimize the drug-target residence time on a structural basis. Using our new, efficient, and widely applicable mechanistically informed kinetic approach, we obtained a full characterization of saFabI inhibition by a series of 20 diphenyl ethers complemented by a collection of 9 saFabI-inhibitor crystal structures. We identified a strong correlation between the affinities of the investigated saFabI diphenyl ether inhibitors and their corresponding residence times, which can be rationalized on a structural basis. Because of its favorable interactions with the enzyme, the residence time of our most potent compound exceeds 10 h. In addition, we found that affinity and residence time in this system can be significantly enhanced by modifications predictable by a careful consideration of catalysis. Our study provides a blueprint for investigating and prolonging drug-target kinetics and may aid in the rational design of long-residence-time inhibitors targeting the essential saFabI enzyme.
Protein fold recognition without Boltzmann statistics or explicit physical basis (1998)

Abstract We present a fast method for finding optimal parameters for a low‐resolution (threading) force field intended to distinguish correct from incorrect folds for a given protein sequence. In contrast to other methods, the parameterization uses information from >10 7 misfolded structures as well as a set of native sequence‐structure pairs. In addition to testing the resulting force field's performance on the protein sequence threading problem, results are shown that characterize the number of parameters necessary for effective structure recognition.
NOVEL PICOLINIC ACID-CONTAINING PYRROLE-IMIDAZOLE POLYAMIDES: SYNTHESIS AND T-G MISMATCHED BASE PAIR RECOGNITION (2005)

Article NOVEL PICOLINIC ACID-CONTAINING PYRROLE-IMIDAZOLE POLYAMIDES: SYNTHESIS AND T-G MISMATCHED BASE PAIR RECOGNITION was published on April 1, 2005 in the journal Heterocyclic Communications (volume 11, issue 2).
Automatic Person Detection and Tracking using Fuzzy Controlled Active Cameras (2007)

This paper presents an automatic system for the monitoring of indoor environments using pan-tilt-zoomable cameras. A combination of Haar-feature classifier-based detection and color histogram filtering is used to achieve reliable initialization of person tracks even in the presence of camera movement. A combination of adaptive color and KLT feature trackers for face and upper body allows for robust tracking and track recovery in the presence of occlusion or interference. The continuous recomputation of camera parameters, coupled with a fuzzy controlling scheme allow for smooth tracking of moving targets as well as acquisition of stable facial close ups, similar to the natural behavior of a human cameraman. The system is tested on a series of natural indoor monitoring scenarios and shows a high degree of naturalness, flexibility and robustness.
Polymerization of lactones, 17. Synthesis of ethylene glycol‐<scp>L</scp>‐lactide block copolymers (1993)

Abstract The anionic polymerization of L ‐lactide in the presence of the sodium poly(ethylene glycol)ate in tetrahydrofuran at 25°C yields “tailored” ABA triblock copolymers having the expected compositions and molecular weights. During the polymerization, a slight racemization of L‐lactide was observed. The water absorption experiments indicate that the introduction of the poly(ethylene glycol) block into the L‐lactide polymer chain markedly increases its hydrophilicity. It was revealed that the morphology and mechanical properties of block copolymers depend strongly on their composition and thermal treatment.
Fluorescent Gas Sensors Based on Nanoporous Optical Resonators (Microcavities) Infiltrated With Sensory Emissive Polymers (2011)

In this contribution, we report fluorescent gas chemosensors for detection of nitroaromatic vapors and other classes of volatile organic compounds based on porous silicon (PSi) microcavity (MC) infiltrated with sensory emissive polymers. Such hybrid functional sensors have several advantages over traditional fluorescent chemosensors, where the sensory polymers are deposited on flat substrate. This includes a high interfacial area of nanoporous Si (high sensitivity), narrow fluorescent band due to photon confinement, dependence of the spectral peak position on nature of analyte (enhanced selectivity), and fast recovery time. We demonstrated that deep and uniform polymer infiltration is critical for effective gas sensing and investigated the experimental conditions required for preparation of high-quality hybrid sensors. In the case of deep infiltration, the broad polymer fluorescence (FWHM ~100 nm) shows a narrowing to the resonance peak (FWHM ~10 nm) with narrow intensity angle diagram. In addition, the potential of the sensor array platform and sensor recovery under ultrasound power is discussed.
요가프로그램이 정신장애여성의 체력과 신체구성에 미치는 영향 (2011)

본 연구의 목적은 신체활동이 절대적으로 부족한 장애인들에게 신체적, 정신적으로 효과적인 요가프로그램을 실시하여 건강 체력과 신체구성성분에 변화를 보여줌으로써 적절한 운동프로그램을 제시하고자 하는데 있다. 정신장애여성을 실험군(n=10)과 대조군(n=10)으로 구분하여 주3회 50-60분 정도의 요가프로그램을 실시하여 다음과 같은 결과를 얻었다. 첫째, 체력 요소 중 지구력, 유연성, 민첩성과 평형성에서 유의한 변화를 보였다. 둘째, 요가프로그램 실시 후 신체구성 성분 중 체중, 체지방량(p=0.001)과 체지방률(p=0.002)에서 매우 유의하게 감소하였고, 두 그룹 간 요가프로그램 실시 후 제지방량(p=0.014)에서 유의하게 증가하였다. 요가프로그램이 일반적으로 알려진 체중감량을 위해 실시하는 유산소성 운동프로그램과 유사한 정도의 운동효과를 보인 것은 눈여겨 볼만한 결과라고 생각한다. 이는 수용시설 하루 일과 프로그램을 살펴보면 많은 시간을 신체활동 프로그램으로 할애하고 있지만 효과적인 운동프로그램이 부족하다는 것을 보여주는 것이다. 본 연구는 신체활동이 부족한 정신 장애인들을 대상으로 한 실험으로 신체구성 성분 중 체지방과 관련된 변인에서 매우 유의한 감소를 보여주고 있다. 이를 통해 신체활동 프로그램을 할 수 없는 장애인들에게 좀 더 적극적으로 운동프로그램을 실시할 수 있도록 다양한 종목의 운동프로그램 개발을 위해 운동의 효과를 규명하는 후속연구가 이루어져야 할 것이다.
ChemInform Abstract: Synthesis of Uracil‐5‐phosphinic and 5‐Thiophosphonic Acids. (1989)

Abstract Reaction of 5‐bromo‐2,4‐dimethoxypyrimidine (I) with butyllithium and diethyl chlorophosphite (II) produces the phosphinate (III) which is hydrolyzed to yield uracil‐5‐phosphinic acid (IV).
Effects of Cyclic Amp and Dibutyryl Cyclic Amp on the Heart and Coronary Circulation (1974)

Using the canine heart-lung preparation supported by a donor dog and the isolated right and left atrial preparations of the guinea-pig and the rat, the cardiac actions of dibutyryl cyclic AMP were studied and compared with those of cyclic. AMP. Dibutyryl cyclic AMP produced positive inotropic and chronotropic effects associated with an increase in the coronary flow, while cyclic AMP produced negative inotropic and chronotropic effects, together with an increase in the coronary flow. Propranolol did not alter the effects of dibutyryl cyclic AMP. The negative inotropic and chronotropic effect of cyclic AMP was antagonized by aminophylline, while the positive inotropic and chronotropic effect of dibutyryl cyclic AMP was potentiated by this compound. Whereas the coronary flow increase by dibutyryl cyclic AMP was associated with an increase in the myocardial oxygen consumption, there was no increase in the myocardial oxygen consumption after cyclic AMP. It is concluded that dibutyryl cyclic AMP passed the surface membrane and exerted its effect after having been converted to cyclic AMP within the cell. Cyclic AMP was presumed to remain outside the cell and produce its effect in the same way as adenine nucleotides and adenosine produce their effect.
DOLL PLAY AS A FUNCTION OF THE REALISM OF THE MATERIALS AND THE LENGTH OF THE EXPERIMENTAL SESSION1 (1945)

Projective doll play has become a widely used clinical procedure for the analysis of children's motivational systems. The fertility and complexity of data secured from it, however, have led its users to devote more consideration to problems of interpretation than to methodology. As a result, many variants of the basic technique have been reported, but no systematic investigation has been made of their effects on performance. If doll play is to become a fruitful, objective method for motivational analysis, detailed study must be made of the influence of important elements in the method itself. The present study was designed to discover the effects, on certain significant aspects of doll play performance, of varying two characteristics of the procedure: kind of materials used and duration of the play session.2 Various opinions have been expressed concerning materials, but for the most part investigators' preferences appear to have been largely a function of theoretical predisposition rather than objective information concerning the influence of different kinds of materials.
Enzyme electrode for the substrate urea (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzyme electrode for the substrate ureaGeorge G. Guilbault and Joseph G. Montalvo Jr.Cite this: J. Am. Chem. Soc. 1970, 92, 8, 2533–2538Publication Date (Print):April 1, 1970Publication History Published online1 May 2002Published inissue 1 April 1970https://pubs.acs.org/doi/10.1021/ja00711a052https://doi.org/10.1021/ja00711a052research-articleACS PublicationsRequest reuse permissionsArticle Views295Altmetric-Citations135LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Metabolic and cytoskeletal modulation of transferrin receptor mobility in mitogen-activated human lymphocytes. (1980)

The transferrin receptors which appear on mitogen-activated human peripheral blood lymphocytes were found by the use of immunofluorescence techniques to display temperature-dependent patching and capping reactions upon binding of transferrin. Lateral mobility of ligand-occupied membrane sites was accompanied by both shedding and endocytosis of receptor-transferrin complexes. In the presence of sodium azide or the microfilament inhibitor cytochalasin B, cap formation and shedding were markedly inhibited. In contrast, endocytosis of patched receptor-ligand complexes was inhibited by azide and microtubule inhibitors, including colchicine, vinblastine and vincristine. Co-capping experiments performed to elucidate further the alterations in membrane configuration involved in these reactions failed to reveal any topographical relationship between transferrin receptors and lectin-binding sites in these cells. These studied indicate that temperature-dependent mobility of transferrin receptors upon mitogen-activated peripheral blood lymphocytes is dependent upon the integrity of the cytoskeletal system and metabolic function of the cell.
America Through Women's Eyes (1934)

America Through Women's Eyes. Edited by Mary R. Beard. (New York: The Macmillan Company, 1933. 558 pp. Bibliography. $3.50.) Get access Kathleen Bruce Kathleen Bruce Hollins CollegeVirginia Search for other works by this author on: Oxford Academic Google Scholar Journal of American History, Volume 20, Issue 4, March 1934, Pages 592–593, https://doi.org/10.2307/1897227 Published: 01 March 1934
Kinetics of the reactions of a zinc ferrite sorbent in high-temperature coal gas desulfurization (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKinetics of the reactions of a zinc ferrite sorbent in high-temperature coal gas desulfurizationMark C. Woods, Santosh K. Gangwal, Douglas P. Harrison, and Kandaswamy JothimurugesanCite this: Ind. Eng. Chem. Res. 1991, 30, 1, 100–107Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/ie00049a015https://doi.org/10.1021/ie00049a015research-articleACS PublicationsRequest reuse permissionsArticle Views266Altmetric-Citations43LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Simulation and experiment on SIW slot array antennas (2004)

By etching longitudinal slots on the top metallic surface of the substrate integrated waveguide (SIW), an integrated slot-array antenna is proposed in this letter. The whole antenna and feeding system are fabricated on a single substrate, which takes the advantage of small size, low profile, and low cost, etc. The design process and experimental results of a four-by-four SIW slot array antenna at X-band are presented.
Instability in lumbar spondylolisthesis: a radiologic study of several concepts (1980)

An attempt was made to define more precisely the notion of spine instability in lumbar spondylolisthesis. By means of lateral radiographs in flexion and extnsion, the axes of movement and the degree of mobility at the L3--L4, L4--L5, and L5--S1 levels were determined in 24 cases of true (spondylolytic) spondylolisthesis. Axes of movement at the spondylolisthetic levels showed a somewhat larger spread in and around the discs than at the normal levels, but instability in the form of parallel displacement of vertebral endplates was not observed. Hypermobility at the spondylolisthetic level proved to be a regular finding. The still current concept of "instability" as presented by Knutsson in 1944 was critically reviewed and denied as valid. Imprecision in the literature regarding instability may partly be attributed to insufficient discrimination between vertebral displacement as anatomic relationship and as abnormal type of movement. By designing a two-dimensional model other movements than flexion-extension could be studied; a special form of instability at the spondylolisthetic level was shown to appear during forward and backward movement of the lumbar spine above it. This detailed analysis facilitates understanding of the effect of different surgical procedures on vertebral movement and instability in lumbar spondylolisthesis.
An Accurate DNA Sensing and Diagnosis Methodology Using Fabricated Silicon Nanopores (2006)

Nanopore-based biomolecular sensing is an emerging nanotechnology which relies on the ability to measure changes in ionic conductance of single nanoscale pores as biomolecular analytes are driven through them, one at a time, by an applied electric field. Nanopores constructed from self-assembled proteins as well as using silicon-based fabrication techniques have been demonstrated to allow sizing and identification of DNA, RNA, proteins, and other biomolecules many times faster than with current technology. Despite the potential of nanopore sensing to produce "next generation" biomolecule analysis devices, its current demonstrations are based on the use of a simple dc stimulus across the nanopore. As a result, the resolution obtained is insufficient for many practical applications. In this paper, we report a novel diagnosis methodology for nanopore sensors based on optimization of a generalized electrical stimulus and a microscopic model of the biomolecule transport process. This methodology is applied to analyze the size distribution of an arbitrary mixture of DNA strands, which is a critical step in DNA sequencing. A transport model for long polymers in nanopores is built and parameterized to reproduce existing experimental data. The electrical stimulus is optimized "on-the-fly" using the model, to obtain a significant increase in the sizing resolution for any given range of DNA sizes and hence a clear identification of all sizes of DNA in the mixture. Hence, it is proposed that nanopore-based DNA sensing can be advanced significantly incurring no (or minimal) hardware overhead, by a combination of optimized stimuli and microscopic transport modeling
Using Word 97 to create documents for an intranet (1998)

Word 97 allows new and existing documents to be saved into html format. This file format (or more accurately coding), is used to mark up documents stored on part of the Internet known as the world wide web, or simply the web. In addition to text, these documents may contain multimedia elements , such as pictures, animation, audio and video, forms linking to information held in a spreadsheet or database, or programs known as applets or controls, which download with a document to run on a local machine. Documents can also contain hyperlinks, sections of text or graphics which when selected by the user, link to other related files. The linked files may be stored on a local or remote computer, the exact nature and location of which is transparent to the user. Documents stored on the web can be accessed using a web browser such as Microsoft's Internet Explorer.
CD-34 Is Expressed by a Distinctive Cell Population in Peripheral Nerve, Nerve Sheath Tumors, and Related Lesions (1993)

The pattern of CD-34 antigen (human progenitor cell antigen) immunoreactivity was studied within normal nerve, and a variety of nerve sheath and neuroectodermal tumors. Besides normal nerves, 111 soft tissue tumors were studied, including 17 neurofibromas, 10 neurilemomas, 12 malignant peripheral nerve sheath tumors, 1 melanocytic schwannoma, 21 fibroblastic lesions, 31 fibrohistiocytic lesions, seven neuroectodermal lesions, and 10 miscellaneous tumors. CD-34-positive dendritic cells were consistently identified within the endoneurium of normal nerve, all neurofibromas, dermatofibrosarcomas, and Antoni B (but not Antoni A) areas of neurilemomas. CD-34 was not expressed in the majority (eight of 10 cases) of malignant peripheral nerve sheath tumors. CD-34 was also lacking in all fibroblastic lesions (nodular fasciitis, fibromatosis, keloid, fibrosarcoma) and in neuroectodermal tumors that are not generally considered to show true nerve sheath differentiation (neurotropic melanoma, clear cell sarcoma, neuroepithelioma). We conclude that CD-34 (or a closely related epitope) defines a normally occurring nerve sheath cell that appears to be cytologically and immunophenotypically distinct from a fibroblast and conventional Schwann cell. The antigen can also be localized to benign nerve sheath tumors, but tends to be lost in malignant ones. The consistent presence of CD-34 within all 13 cases of dermatofibrosarcoma protuberans can be used as evidence in support of the view that these lesions are variants of nerve sheath tumors, and distinct from benign fibrous histiocytomas which consistently lack the antigen. Finally, expression of CD-34 by one of three giant cell fibroblastomas reinforces the close relationship between this tumor and dermatofibrosarcoma protuberans.