gebhart/patrepeval huggingface dataset:
Peptide‐Based Nanotubes and Their Applications in Bionanotechnology (2005)

In nature, biological nanomaterials are synthesized under ambient conditions in a natural microscopic-sized laboratory, such as a cell. Biological molecules, such as peptides and proteins, undergo self-assembly processes in vivo and in vitro, and these monomers are assembled into various nanometer-scale structures at room temperature and atmospheric pressure. The self-assembled peptide nanostructures can be further organized to form nanowires, nanotubes, and nanoparticles via their molecular-recognition functions. The application of molecular self-assemblies of synthetic peptides as nanometer-scale building blocks in devices is robust, practical, and affordable due to their advantages of reproducibility, large-scale production ability, monodispersity, and simpler experimental methods. It is also beneficial that smart functionalities can be added at desired positions in peptide nanotubes through well-established chemical and peptide syntheses. These features of peptide-based nanotubes are the driving force for investigating and developing peptide nanotube assemblies for biological and non-biological applications.
A Rapid Seedling Bioassay for the Study of Allelopathy (1987)

A bioassay was developed to evaluate the biological activity of pure allelopathic compounds qualitatively and quantitatively and to monitor activity of interest during the purification of chemical components in extracts. The effects of allelopathic compounds and analogs on various growth parameters (GP) were measured using the test species Echinochloa crusgalli (L.) Beauvois and Sesbania exaltata (Raf.) Cory. Root length and root fresh weight were the most sensitive GP, but these measurements were extremely time consuming. Therefore, a model was developed to ascertain predicted shoot-plus-root fresh weight (PSRFW) from total plant fresh weight, which is less sensitive due to seed weight, but rapidly measured. It was concluded that a seedling bioassay using PSRFW is an efficient method to evaluate the phytotoxicity of biological samples.
BJT class-F power amplifier near transition frequency (2005)

This paper presents the analysis and the design rules of bipolar junction transistor (BJT) class-F power amplifier operating at a frequency comparable with the transition one. Since the impulse of collector current becomes stretched with operation frequency increasing, its spectral content is changed. Thus, the requirements for the amplifier's output network and bias conditions should be corrected compared with the classic low-frequency case in order to achieve high-efficiency class-F operation. Using the simple BJT model, these new requirements were obtained in this paper. The presented results of simulation and experimental verification show the advantages of proposed approach.
A Rapid Procedure for Cloning Genes from λ Libraries by Complementation of <i>E. coli</i> Defective Mutants: Application to the <i>fabE</i> Region of the <i>E. coli</i> Chromosome (1989)

I describe a general and rapid procedure allowing the isolation of specialized lambda transducing phages from a lambda library by lysogenic complementation of defective mutants of Escherichia coli. As an example, the cloning of the E. coli fabE gene and of two other adjacent genetic determinants is presented. Subcloning and determination of its nucleotide sequence reveals that fabE codes for the biotin carboxyl carrier protein (BCCP), one of the three subunits of acetyl coenzyme A carboxylase.
The unresponsiveness of aged mice to polysaccharide antigens is a result of a defect in macrophage function (2005)

A reduction in macrophage (MPhi) function with aging makes mice less responsive to bacterial capsular polysaccharides, such as those present in the pneumococcal polysaccharide vaccine, a model of thymus independent (TI) antigen (Ag). Using trinitrophenol (TNP)-lipopolysaccharide (LPS) and TNP-Ficoll, two other well-studied TI Ag, we studied the mechanistic basis of reduced MPhi function in the aged. We show that aged mice are profoundly hyporesponsive to these TI Ag. As a result of a requirement for MPhi, highly purified B cells from young-adult mice do not respond to TI Ag. When purified, young B cells were immunized with TNP-Ficoll, the antibody production from those cultures reconstituted with MPhi from aged mice was significantly lower than that seen with young MPhi. Consequently, this unresponsiveness can be overcome by a mixture of interleukin (IL)-1beta and IL-6. Upon stimulation with LPS, in comparison with young MPhi, aged MPhi secreted reduced amounts of IL-6, tumor necrosis factor alpha, IL-1beta, and IL-12, cytokines necessary for B cells to respond to TI Ag. LPS also induced aged MPhi to produce an excess of IL-10. Neutralization of IL-10 enhanced the production of proinflamatory cytokines by MPhi upon LPS stimulation and also induced Ab production by aged splenocytes. Thus, the inability of aged MPhi to help the B cell response appears to be caused by an excess of IL-10. As aged MPhi have a reduced number of cells expressing Toll-like receptor 4 and CD14, the imbalance in cytokine production might be partly a result of fewer cells expressing key components of the LPS receptor complex.
Management of Neurogenic Bladders Secondary to Cord Injury (1963)

No AccessJournal of Urology1 Aug 1963Management of Neurogenic Bladders Secondary to Cord Injury Chaules L. Reynolds Chaules L. ReynoldsChaules L. Reynolds More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)64383-0AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail © 1963 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 90Issue 2August 1963Page: 172-174 Advertisement Copyright & Permissions© 1963 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Chaules L. Reynolds More articles by this author Expand All Advertisement Loading ...
Improving organic transistor performance with Schottky contacts (2004)

Organic field-effect transistors (OFETs) with non-Ohmic contacts, e.g., pentacene with gold electrodes, exhibit a linearly growing threshold voltage with increased film thickness due to tunnel injection [R. Schroeder et al., Appl. Phys. Lett. 83, 3201 (2003)]. In this letter, we demonstrate gold/pentacene OFETs with a low threshold voltage independent of pentacene thickness. By doping the pentacene in the contact area with FeCl3 (iron-III-chloride), the metal-insulator-type tunneling barrier was changed to a metal-semiconductor Schottky barrier. Since the injection through a Schottky barrier depends on the potential and not on the electric field, the threshold voltage is no longer a function of the semiconductor thickness. Through selective doping of the area under the electrode, the channel remains undoped, and large on/off ratios are retained.
Quantification of DNA in simple eukaryotic cells using Fourier transform infrared spectroscopy (2012)

A technique capable of detecting and monitoring nucleic acid concentration offers potential in diagnosing cancer and further developing an understanding of the biochemistry of disease. The application of Fourier transform infrared (FTIR) spectroscopy has previously been hindered by the supposed non-Beer-Lambert absorption behavior of DNA in intact cells making elucidation of the DNA bands difficult. We use known composition DNA/hemoglobin standards to successfully estimate the DNA content in avian erythrocyte nuclei (44.2%) and intact erythrocytes (12.8%). Furthermore we demonstrate that the absorption of cellular DNA does follow the Beer-Lambert Law and highlights the role of conformation and hydration in FTIR spectroscopy of biological samples.
Polymorphisms in interleukin‐1 receptor‐associated kinase 4 are associated with total serum IgE (2009)

These results demonstrate a clear association between polymorphisms in the IRAK4 gene and serum IgE levels in patients with CRS and asthma. IRAK4 may be important in the regulation of IgE levels in patients with inflammatory diseases of the airways.
Hybrid calibration methodology for building energy models coupling sensor data and stochastic modeling (2013)

Calibrated detailed energy models are often used to identify economic deep retrofit opportunities in existing buildings. But the uncertainty in building performance makes it technically unrealistic to reach a single “best fit” model without extensive sub-metering and domain experts on the project which is typically labor intensive. To address this problem, researchers have been adopting stochastic modeling as a more reliable approach to calibrate building energy models. A set of all plausible models is found, rather than a best fit model, which accounts for uncertainties in existing building properties and conditions. In addition, sensor data collected from within a building can be used to identify key operational characteristics such as setpoint temperatures, carbon-dioxide levels, light levels, and temperature setbacks. This paper presents a hybrid calibration methodology for building energy models using a combination of short-term wireless sensor data, 15-min interval smart meter data and stochastic modeling. The hybrid approach provides a means to calibrate the operational variables and physical variables separately, reducing potential bias and errors and to reach a set of plausible model solutions. A case study is presented to demonstrate the strength of the calibration methodology.
Changes in the stretch reflex of the human first dorsal interosseous muscle during different tasks. (1992)

1. Subjects flexed the interphalangeal joint of the index finger against a lever which was mounted on the shaft of a torque motor. 2. There were two different tasks. In one, the subject attempted to maintain a constant finger position in the face of changing forces, whereas in the other the subject attempted to maintain a constant force while the motor moved the lever. 3. Each of the tasks was interrupted by ramp extensions. These evoked stretch reflexes which were recorded in the first dorsal interosseous (FDI) muscle electromyogram (EMG). 4. Long-latency (55-90 ms) reflex responses were larger during the 'maintain position' task than during the 'maintain force' task, although the ramp extensions began from a similar finger position, a similar flexing force, and with a similar amount of FDI EMG activity. 5. It is concluded that the nature of the task has an effect on the magnitude of the long-latency stretch reflex.
Vascular endothelial growth factor receptor-1 mediates migration of human colorectal carcinoma cells by activation of Src family kinases (2006)

Vascular endothelial growth factor (VEGF) is the predominant pro-angiogenic cytokine in human malignancy, and its expression correlates with disease recurrence and poor outcomes in patients with colorectal cancer. Recently, expression of vascular endothelial growth factor receptors (VEGFRs) has been observed on tumours of epithelial origin, including those arising in the colon, but the molecular mechanisms governing potential VEGF-driven biologic functioning in these tumours are not well characterised. In this report, we investigated the role of Src family kinases (SFKs) in VEGF-mediated signalling in human colorectal carcinoma (CRC) cell lines. Vascular endothelial growth factor specifically activated SFKs in HT29 and KM12L4 CRC cell lines. Further, VEGF stimulation resulted in enhanced cellular migration, which was effectively blocked by pharmacologic inhibition of VEGFR-1 or Src kinase. Correspondingly, migration studies using siRNA clones with reduced Src expression confirmed the requirement for Src in VEGF-induced migration in these cells. Furthermore, VEGF treatment enhanced VEGFR-1/SFK complex formation and increased tyrosine phosphorylation of focal adhesion kinase, p130 cas and paxillin. Finally, we demonstrate that VEGF-induced migration is not due, at least in part, to VEGF acting as a mitogen. These results suggest that VEGFR-1 promotes migration of tumour cells through a Src-dependent pathway linked to activation of focal adhesion components that regulate this process.
Implementation of Discontinuous Galerkin (DG) method for antenna applications (2007)

In this paper, we have presented representative applications of the DG based TEMPUS solver for modeling and simulation of complex broadband antennas. Numerical results demonstrate the excellent performance of the code in typical antenna applications such as monopole, microstrip and cavity-backed slots. The code has a powerful underlying structure enabling its applicability to large and complex geometry problems in antennas and scattering.
Sobre la variedad de aproximaciones mentalistas al lenguaje. Comparación de enfoques y perspectivas de encuentro (2021)

En este artículo revisamos dos aproximaciones mentalistas al estudio del lenguaje: el enfoque generativista y el cognitivista. En primer lugar, exponemos los supuestos básicos de la lingüística generativa, centrada en el estudio formal de las propiedades computacionales del lenguaje, y, en segundo lugar, los comparamos con los de la corriente alternativa de la lingüística cognitiva, según la cual el lenguaje no se analiza como una propiedad computacional específica de la mente humana sino más bien como una capacidad que participa de los principios generales de la cognición. Una vez presentados los distintos fundamentos teóricos de ambas corrientes, ofrecemos algunas perspectivas de encuentro entre ellas. El trabajo concluye con un llamamiento a la necesidad de establecer puentes de diálogo entre los diferentes enfoques para superar los retos que plantean los diferentes avances en las ciencias del lenguaje y la ciencia cognitiva en general.
ALL-1 gene rearrangements in DNA topoisomerase II inhibitor-related leukemia in children (1995)

We examined clinical, morphologic, and cytogenetic features and ALL-1 (MLL, Htrxl, HRX) gene rearrangements in 17 cases of secondary leukemia that occurred 11 months to 9 years from diagnoses of primary cancers in children who received topoisomerase II inhibitors or developed secondary leukemias typical of those associated with this therapy. Primary diagnoses included nine solid tumors and eight leukemias. Ten secondary leukemias were acute myeloid leukemia (AML), one was of mixed lineage, two were acute lymphoblastic leukemia (ALL), and four presented as myelodysplasia. Of 15 cases with 11q23 involvement, 11 (73%) were cytogenetically identifiable; four cases had molecular rearrangement only. By Southern blot, rearrangements within the ALL-1 gene were similar to sporadic cases. The results of this analysis suggest the following: (1) In most pediatric cases of topoisomerase II inhibitor-associated leukemia, there is disruption of the breakpoint cluster region of the ALL-1 gene at chromosomal band 11q23. (2) Exposure histories vary in secondary 11q23 leukemia, as the only topoisomerase II inhibitor was dactinomycin in one case, and, in another case, no topoisomerase II inhibitor was administered. (3) There is clinical, morphologic, cytogenetic, and molecular heterogeneity in pediatric secondary 11q23 leukemia. (4) There are some survivors of pediatric secondary 11q23 leukemia, but the outcome is most often fatal.
Semantic Ideation Learning for Agent-Based E-Brainstorming (2008)

Brainstorming can assist organizations in generating creative ideas using teamwork and collaboration. However, the role of information technology in brainstorming is merely that of an assistant that passively supports the progression of brainstorming sessions rather than proactively engaging in the sessions. This paper integrates the unique association thinking of humans with an intelligent agent technique to devise an automated decision agent called the Semantic Ideation Learning Agent (SILA) that can represent a session participant who is actively participating in brainstorming. SILAs are grounded on the three association capabilities of human thinking (similarity, contiguity, and contrast). Furthermore, a Collective Brainstorming Decision System (CBDS) is built to construct an environment where SILAs can learn and share their knowledge with each other. Additionally, CBDS is integrated into an intelligent care project (iCare) for the purpose of innovated e-service recommendation. Preliminarily, evaluation results indicate that the proposed system advances e-brainstorming by crossing the three key boundaries of human ideation capability (understanding, cognition boundary, and endurance).
Jamming Resilient Communication Using MIMO Interference Cancellation (2016)

Jamming attack is a serious threat to the wireless communications. Reactive jamming maximizes the attack efficiency by jamming only when the targets are communicating, which can be readily implemented using software-defined radios. In this paper, we explore the use of the multi-input multi-output (MIMO) technology to achieve jamming resilient orthogonal frequency-division multiplexing (OFDM) communication. In particular, MIMO interference cancellation treats jamming signals as noise and strategically cancels them out, while transmit precoding adjusts the signal directions to optimize the decoding performance. We first investigate the reactive jamming strategies and their impacts on the MIMO-OFDM receivers. We then present a MIMO-based anti-jamming scheme that exploits MIMO interference cancellation and transmit precoding technologies to turn a jammed non-connectivity scenario into an operational network. We implement our jamming resilient communication scheme using software-defined radios. Our testbed evaluation shows the destructive power of reactive jamming attack, and also validates the efficacy and efficiency of our defense mechanisms in the presence of numerous types of reactive jammers with different jamming signal powers.
Functional expression of Na(+)-dependent nucleoside transport systems of rat intestine in isolated oocytes of Xenopus laevis. Demonstration that rat jejunum expresses the purine-selective system N1 (cif) and a second, novel system N3 having broad specificity for purine and pyrimidine nucleosides. (1993)

Isolated stage VI oocytes from Xenopus laevis expressed uridine transport activity after microinjection of mRNA from rat jejunum. Uridine uptake during 30 min (10 microM, 20 degrees C) by mRNA-injected oocytes reached 2.5 pmol/oocyte, compared with endogenous uptake by water-injected oocytes of about 0.05 pmol/oocyte. The expressed transport activity was 96% Na(+)-dependent, saturable (apparent Km = 15 microM) and inhibited by phloridzin (IC50 = 100 microM). Nucleoside inhibition studies resolved the expressed transport activity into two components: 1) a novel Na(+)-dependent system of broad purine and pyrimidine specificity that was inhibited by low concentrations of guanosine, inosine, adenosine, uridine, thymidine, and cytidine and 2) a Na(+)-dependent system of narrower specificity that was inhibited by low concentrations of guanosine, inosine, adenosine, and uridine and by high concentrations of thymidine and cytidine. The characteristics of the latter system are consistent with those of the Na(+)-dependent nucleoside transport system N1 (cif), previously identified in a number of cell types and tissues, including intestinal epithelia and cultured cells of intestinal origin. The broad specificity system, which was also detected in mRNA-injected oocytes using thymidine as permeant, has been given the provisional designation N3 to distinguish it from the previously described N1 (purine-selective) and N2 (pyrimidine-selective) Na(+)-linked nucleoside transporters. Rat jejunal transporters N1 and N3 were both expressed maximally by the same mRNA size fraction (1.6-3.0 kb, peak 2.3 kb).
Molecular basis of age-dependent gastric inactivation of rhesus rotavirus in the mouse. (1992)

Rotavirus requires specific proteolytic activation by trypsin for efficient replication in tissue culture. To observe the nature of intestinal proteolytic activation of rotavirus in vivo, metabolically labeled rhesus rotavirus (RRV) grown in the presence of trypsin inhibitors was administered to adult and 10-d-old suckling mice by gavage. In the adult stomach, vp4 was cleaved in a manner distinct from in vitro trypsin cleavage. In the suckling stomach, RRV vp4 remains largely uncleaved. The alternative cleavage in the adult stomach was associated with a profound decrease in viral infectivity. vp4 from RRV recovered from the suckling small intestinal lumen was cleaved in a pattern similar or identical to in vitro trypsin-activated virus with bands comigrating with vp5* and vp8*. In contrast, vp4 was not observed in any recognizable form in RRV recovered from adult intestines. Comparison of infectivity of virus recovered from suckling and adult intestines revealed a 10,000-fold decrease in titer in the virus recovered from the adult intestine. In vitro digestions of RRV revealed that pepsin digestion can cleave RRV vp4 and markedly enhance acid-induced loss of rotavirus infectivity. Subsequent digestion with chymotrypsin removes most of the pepsin cleavage products of vp4. Virus injected directly into jejunal loops of adult mice and virus administered orally to adult mice pretreated with antiacid drugs retained infectivity. These studies indicate the development of gastric acid and pepsin secretion may be an important host defense factor in rotavirus gastroenteritis.
Hybrid analysis of three-dimensional structures by the method of lines using novel nonequidistant discretization (2003)

Many microwave structures contain various elements with very big difference in their size. Exact modeling of such structures with equidistant discretization requires considerable numerical effort and large memory capacity. Alternative formulas for nonequidistant discretization are proposed. New difference operators for nonequidistant discretization are given. The accuracy of these operators is compared with the previous ones used with the Method of Lines. Numerical results of two microwave filters are presented to check the accuracy of the new algorithm.
Efficacy of 4-allylanisole-based products for protecting individual loblolly pines from <i>Dendroctonus frontalis</i> Zimmermann (Coleoptera: Scolytidae) (2004)

We evaluated the effectiveness of 4-allylanisole (4AA) as a protective treatment for loblolly pines threatened by the southern pine beetle, Dendroctonus frontalis Zimmermann. Three products were evaluated in combination with two methods that promoted attack of trees by D. frontalis. One method used attractive semiochemicals and the other decreased host resistance, both of which are important scenarios for implementing protective treatments of individual trees. Each method promoted mass attack of unprotected control trees, thus providing a statistically verifiable challenge to the candidate protectants. In trees with increased susceptibility, mortality ranged from 63% (untreated) to 77% (4AA applied in paintball formulation), and two products appeared to alter the relative composition of scolytid species that attacked at two heights; however, tree mortality was unaffected. In trees challenged with semiochemical attractants, mortality ranged from 54% (4AA released from vials) to 82% (untreated and paintball application of 4AA). Although 4AA consistently reduces catch of D. frontalis in traps, it was not efficacious for protecting individual loblolly pines over a period of 30 or 60 days in this experiment.