gebhart/patrepeval huggingface dataset:
Nerve growth factor enhances voltage‐gated Na<sup>+</sup> channel activity and Transwell migration in Mat‐LyLu rat prostate cancer cell line (2006)

The highly dynamic nature of voltage-gated Na+ channel (VGSC) expression and its controlling mechanism(s) are not well understood. In this study, we investigated the possible involvement of nerve growth factor (NGF) in regulating VGSC activity in the strongly metastatic Mat-LyLu cell model of rat prostate cancer (PCa). NGF increased peak VGSC current density in a time- and dose-dependent manner. NGF also shifted voltage to peak and the half-activation voltage to more positive potentials, and produced currents with faster kinetics of activation; sensitivity to the VGSC blocker tetrodotoxin (TTX) was not affected. The NGF-induced increase in peak VGSC current density was suppressed by both the pan-trk antagonist K252a, and the protein kinase A (PKA) inhibitor KT5720. NGF did not affect the Nav1.7 mRNA level, but the total VGSC alpha-subunit protein level was upregulated. NGF potentiated the cells' migration in Transwell assays, and this was not affected by TTX. We concluded that NGF upregulated functional VGSC expression in Mat-LyLu cells, with PKA as a signaling intermediate, but enhancement of migration by NGF was independent of VGSC activity.
Dystonia-associated mutations cause premature degradation of torsinA protein and cell-type-specific mislocalization to the nuclear envelope (2008)

An in-frame 3 bp deletion in the torsinA gene resulting in the loss of a glutamate residue at position 302 or 303 (torsinA DeltaE) is the major cause for early-onset torsion dystonia (DYT1). In addition, an 18 bp deletion in the torsinA gene resulting in the loss of residues 323-328 (torsinA Delta323-8) has also been associated with dystonia. Here we report that torsinA DeltaE and torsinA Delta323-8 mutations cause neuronal cell-type-specific mislocalization of torsinA protein to the nuclear envelope without affecting torsinA oligomerization. Furthermore, both dystonia-associated mutations destabilize torsinA protein in dopaminergic cells. We find that wild-type torsinA protein is degraded primarily through the macroautophagy-lysosome pathway. In contrast, torsinA DeltaE and torsinA Delta323-8 mutant proteins are degraded by both the proteasome and macroautophagy-lysosome pathways. Our findings suggest that torsinA mutation-induced premature degradation may contribute to the pathogenesis of dystonia via a loss-of-function mechanism and underscore the importance of both the proteasome and macroautophagy in the clearance of dystonia-associated torsinA mutant proteins.
A survey on indoor positioning systems (2014)

This paper aims to provide the reader with a review of the main technologies explored in the literature to solve the indoor localization issue. Furthermore, some systems that use these enabling technologies in real-world scenarios are presented and discussed. This could deliver a better understanding of the state-of-the-art and motivate new research efforts in this promising field. Finally, focusing on one of the major challenges in the indoor localization field, i.e., the indoor animal tracking, existing indoor tracking systems have been reviewed and compared by analyzing advantages and drawbacks.
Your personal virtual heart (2014)

A poet might say that each human being's heart is a unique mystery. Those of us working in the brand new field of computational medicine, however, can now model each of those unique hearts with marvelous accuracy and reveal their secrets. In my laboratory at Johns Hopkins University, my team creates computer models to simulate individual patients' hearts, which can help cardiologists carry out life-saving treatments. Such models may soon transform medicine, ushering in a new kind of personalized health care with radically improved outcomes. · Biomedical engineers have learned how to use numerical models to generate increasingly sophisticated "virtual organs" over the past decade, and rapid developments in cardiac simulation have made the virtual heart the most complete model of all. It's a complex replica, as it must mimic the heart's workings at the molecular scale, through the cellular scale, and up to the level of the whole organ, where muscle tissue expands and contracts with every heartbeat. What's more, the modeling at these different scales must be tightly integrated to accurately render the constant feedback interactions that govern the functions of the heart.
KLF8 promotes human breast cancer cell invasion and metastasis by transcriptional activation of MMP9 (2010)

Epithelial to mesenchymal transition (EMT) and extracellular matrix degradation are critical for the initiation and progression of tumor invasion. We have recently identified Krüppel-like factor 8 (KLF8) as a critical inducer of EMT and invasion. KLF8 induces EMT primarily by repressing E-cadherin transcription. However, how KLF8 promotes invasion is unknown. Here, we report a novel KLF8-to- matrix metalloproteinase (MMP)9 signaling that promotes human breast cancer invasion. To identify the potential KLF8 regulation of MMPs in breast cancer, we established two inducible cell lines that allow either KLF8 overexpression in MCF-10A or knockdown in MDA-MB-231 cells. KLF8 overexpression induced a strong increase in MMP9 expression and activity as determined by quantitative real-time PCR and zymography. This induction was well correlated with the MMP inhibitor-sensitive Matrigel invasion. Conversely, KLF8 knockdown caused the opposite changes that could be partially prevented by MMP9 overexpression. Promoter-reporter assays and chromatin and oligonucleotide precipitations determined that KLF8 directly bound and activated the human MMP9 gene promoter. Three-dimensional (3D) glandular culture showed that KLF8 expression disrupted the normal acinus formation, which could be prevented by the MMP inhibitor, whereas KLF8 knockdown corrected the abnormal 3D architecture, which could be protected by MMP9 overexpression. KLF8 knockdown promoted MDA-MB-231 cell aggregation in suspension culture, which could be prevented by MMP9 overexpression. KLF8 knockdown inhibited the lung metastasis of MDA-MB-231 cells in nude mice. Immunohistochemical staining strongly correlated the co-expression of KLF8 and MMP9 with the patient tumor invasion, metastasis and poor survival. Taken together, this work identified the KLF8 activation of MMP9 as a novel and critical signaling mechanism underlying human breast cancer invasion and metastasis.
Multiple Selection Criteria in Soybeans<sup>1</sup> (1959)

Synopsis Selection indexes involving 15 combinations of 6 characters were computed for 2 populations of soybeans. For a 1:.6 price ratio of oil to protein, the advances expected from selection based on oil and seed weight were 101 and 118% of those based on oil, protein, and yield in populations 1 and 2, respectively; and 84 and 95% of those based on all 6 characters.
Increased Reactivity to a Met-Enkephalin Analogue in the Control of Autonomic Responses in Migraine Patients (1990)

The effects of naloxone and a met-enkephalin analogue on head pain, vascular responses, and autonomic-associated symptoms were studied in 24 migraine patients, 12 patients suffering from tension-type headache, and 24 normal subjects in whom headache was induced by intravenous injections of increasing doses of histamine (histamine test). A hypersensitivity to histamine was found in migraine patients. Naloxone slightly increased the intensity of pain in migraine and tension-type headache sufferers. The met-enkephalin analogue did not affect the intensity of pain in migraine patients, tension-type headache patients, and normal subjects, but it reduced the intensity and duration of facial flushing (p less than 0.001) and the autonomic symptoms (p less than 0.001) in migraine patients when the pretreatment was not given shortly before histamine. In migraine patients, there seems to be an increased reactivity (receptor supersensitivity?) to the met-enkephalin analogue at the level of systems that inhibit facial vasodilatation and autonomic symptoms.
Fabrication of a Wideband Antenna on a Low-Resistivity Silicon Substrate Using a Novel Micromachining Technique (2007)

Fabrication of a wideband antenna on a low-resistivity silicon substrate using a novel micromachining technique is described. The wideband antenna is based on a differential-fed dipole based design that is fabricated on a modified silicon substrate and this eliminates problems associated with antenna efficiency and strength. Results from our fabricated antenna reveal that it resonates at around 5.8 GHz and has a bandwidth of more than 43% making it potentially suitable for IEEE 802.11a and ultrawideband (UWB) applications. The size of the antenna is 12 mm times 2.8 mm times 0.4 mm. Furthermore, the fabricated antenna exhibits an efficiency of more than 20% and has a gain of - 1.35 dBi, which is good for a compact antenna fabricated on a low resistivity silicon substrate.
Deterministic Domain Wall Motion Orthogonal To Current Flow Due To Spin Orbit Torque (2015)

Spin-polarized electrons can move a ferromagnetic domain wall through the transfer of spin angular momentum when current flows in a magnetic nanowire. Such current induced control of a domain wall is of significant interest due to its potential application for low power ultra high-density data storage. In previous reports, it has been observed that the motion of the domain wall always happens parallel to the current flow - either in the same or opposite direction depending on the specific nature of the interaction. In contrast, here we demonstrate deterministic control of a ferromagnetic domain wall orthogonal to current flow by exploiting the spin orbit torque in a perpendicularly polarized Ta/CoFeB/MgO heterostructure in presence of an in-plane magnetic field. Reversing the polarity of either the current flow or the in-plane field is found to reverse the direction of the domain wall motion. Notably, such orthogonal motion with respect to current flow is not possible from traditional spin transfer torque driven domain wall propagation even in presence of an external magnetic field. Therefore the domain wall motion happens purely due to spin orbit torque. These results represent a completely new degree of freedom in current induced control of a ferromagnetic domain wall.
Diffusion anisotropy changes in the brains of professional boxers. (2006)

Increased BD(av) and the decreased FA in the CC and IC may represent preclinical signs of subtle brain injury in professional boxers.
Secuencias inferopaleolíticas en la cuenca media del Duero (2000)

Síntesis de posibles secuencias de terrazas fluviales pleistocénicas, en la cuenca del Duero, con industrias líticas datables en el Paleolítico Inferior.
Subsurface transport of contaminants (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubsurface transport of contaminantsJohn F. McCarthy and John M. ZacharaCite this: Environ. Sci. Technol. 1989, 23, 5, 496–502Publication Date (Print):May 1, 1989Publication History Published online1 May 2002Published inissue 1 May 1989https://pubs.acs.org/doi/10.1021/es00063a001https://doi.org/10.1021/es00063a001research-articleACS PublicationsRequest reuse permissionsArticle Views3145Altmetric-Citations113LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Isolation of human monoclonal antibodies binding to B fragment of diphtheria toxin (1992)

Human tonsillar lymphocytes immunized in vitro with diphtheria toxoid were fused with the heteromyeloma cell line SP2/HPT. Hybrids were selected on hypoxanthine-thymidine aminopterin (HAT) medium and assayed for antibody production both by ELISA and Western blotting. Four stable hybridoma cell lines producing 3-7 micrograms/mL of human IgG1 antibodies were isolated. In Western blotting studies these antibodies show binding to the B fragment of diphtheria toxin. Cells from the subclone P2-13/G35 were injected i.p. in pristane-primed nude mice, and ascites fluid was collected after 4-6 weeks. The monoclonal antibody neutralized the cytotoxicity of diphtheria toxin in the Vero cell assay system. This human monoclonal antibody may have a potential in the immunotherapy of diphtheric disease.
[Feasibility of in vivo isotope labeled autologous mononuclear bone marrow cells monitoring post cardiac transplantation in a swine model of myocardial infarction]. (2010)

Distribution and viability of stem cell after cardiac transplantation could be effective monitored by 18F-FDG labeled autologous mononuclear bone marrow cell technique in acute stage in this model.
RNase E: still a wonderfully mysterious enzyme (1997)

Ribonuclease E (RNase E), which is encoded by an essential Escherichia coli gene known variously as rne, ams, and hmp, was discovered initially as an rRNA-processing enzyme but it is now known to have a general role in RNA decay. Multiple functions, including the ability to cleave RNA endonucleolytically in AU-rich single-strand regions, RNA-binding capabilities, and the ability to interact with polynucleotide phosphorylase and other proteins implicated in the processing and degradation of RNA, are encoded by its 1,061 amino acid residues. The presence of homologues and functional analogues of the rne gene in a variety of prokaryotic and eukaryotic species suggests that its functions have been highly conserved during evolution. While much has been learned in recent years about the structure and functions of RNase E, there is continuing mystery about possible additional activities and molecular interactions of this enzyme.
Kalman filter based localization and tracking estimation for HIMR RFID systems (2018)

Fine-scale RFID motion capture and localization systems are important for emerging applications such as robotic wayfaring, virtual reality, remote motion capture, and tracking. Among all RFID localization and tracking systems, hybrid schemes have the highest accuracy (5-400 cm) and largest range (hundreds of meters) by combining a variety of sources. Recently, linear estimators have been introduced to hybrid inertial microwave reflectometry (HIMR) RFID systems for location and tracking estimation. In this paper, we add a Kalman filter to the output of the original HIMR estimator as a way to refine the precision of the technique. Simulation results show that compared to sensor outputs, fixed-gain and variable-gain versions of the Kalman filter track position much more accurately, with around 5 mm of rms error at a range of 1.2 m.
Concise Syntheses of (−)-Galanthamine and (±)-Codeine via Intramolecular Alkylation of a Phenol Derivative (2009)

Suzuki coupling of 7 to 8 gave the biphenyl derivative 9. Reaction of 9 with ethyl vinyl ether/bromine/base gave 10, which on treatment with CsF/DMF at 130 degrees C resulted in the cross-conjugated 2,5-cyclohexadienone 6. Acid hydrolysis of 6 gave 11, which was reductively aminated to give (+/-)-narwedine 2. Since 2 has been converted into (-)-galanthamine 1 in two steps, this synthesis proceeds in eight steps with an overall yield of 63%. Also treatment of the cross-conjugated cyclohexadienone 6 with nitromethane/base gave 12, which was reduced to provide 13. Reduction of the nitro group in 13 to an amine, followed by reductive amination under acidic conditions, arrives at the codeine skeleton 15. Elaboration of 15 into (+/-)-codeine proceeds via the previously unknown alpha-epoxide derivative 18. This is the shortest synthesis of codeine (13 steps, 20% overall yield) and, for the first time, allows access to codeine without having to reduce codeinone.
JASMONATES INDUCE APOPTOSIS AND CELL CYCLE ARREST IN NON-SMALL CELL LUNG CANCER LINES (2006)

The jasmonates, cis-jasmone (CJ) and methyl jasmonate (MJ), were investigated for their effects against NSCLC cell lines A549 and H520. CJ or MJ inhibited the proliferation of both cell lines in a dose-dependent manner as well as induced cell cycle arrest in the G2/M phase. Apoptosis was observed following treatment with CJ or MJ as indicated by Hoechst staining and confirmed by dual annexin V-fluorescein isothiocyanate (FITC)/prodium iodide (PI) and DAPI (4',6-diamidine-2'-phenylindole dihydrochloride) staining. p38 and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation was observed with increased expression of bax, p21, and caspase-3 activity. These observations indicate that jasmonates may have a therapeutic value in the treatment of lung cancer.
Evolution of Five Anthocyanidin-3-Glucosides in the Skin of the Tempranillo, Moristel, and Garnacha Grape Varieties and Influence of Climatological Variables (1992)

Anthocyanins in skins of Tempranillo [Valdepeñas (California)], Moristel, and Garnacha (Grenache) skin grape varieties were separated and quantified by HPLC in two different vintages (1987 and 1988). The relationship between this evolution and the climatology data for the sampled vineyards was studied. The changes in the anthocyanin content were greater between the same varieties in different years than the different varieties in the same year. Nevertheless, differences were found between varieties. The delphinidin- and petunidin-3-glucosides were found in much higher relative content in Tempranillo grapes than in Garnacha and Moristel. Garnacha presents a higher relative content in cyanidin- and peonidin-3-glucosides, while Moristel presents higher malvidin-3-glucoside relative content.
Evidence of intra‐ and extracellular modifications of monoclonal IgG polypeptide chains generating charge heterogeneity (1998)

The heavy and light chains of IgG monoclonal antibodies (mAbs) can be shown to be heterogeneous, with respect to isoelectric points, when analyzed by two-dimensional electrophoresis (2-DE). The molecular basis for this charge heterogeneity has not been clearly defined but it has been suggested that it could be due, in part, to differences in glycosylation. To investigate this possibility we have compared the 2-DE pattern of glycosylated and aglycosylated forms of the mouse IgG1 mAb (1B7-11), produced in vitro in the presence and absence of tunicamycin. Charge heterogeneity was shown not to be a consequence of glycosylation status. Intracellular and secreted IgG mAbs were also analyzed to investigate the time course of change in charge properties of the heavy and light chains. The charge heterogeneity was found to be generated intracellularly, and alterations in charge properties could be induced during incubation under physiological conditions. Semilogarithmic plots of the density of the principal heavy and light chain spots against incubation time showed linear relationships, suggesting that the charge shifts result from a first-order reaction. The semilogarithmic plot for the light chain correlated well with the time after IgG synthesis. These results suggest that the charge heterogeneity of an IgG mAb is due to intra- and extracellular modifications of the polypeptide chains which reflect "aging" of antibody molecules.
Protein-Rich Residue from Wheat Alcohol Distillation: Fractionation and Characterization (1984)

Cereal Chern. 61(5): 423-427 Whole wheat and flour were fermented to make alcohol. The residue, after alcohol was distilled, was fractionated into distillers' grains, centrifuged solids, and distillers' solubles. Distillers' grains and centrifuged solids had protein contents of 29 and 57%, respectively, and accounted for Fermentation of cereal grains to make alcohol produces a protein-rich material (stillage) after the alcohol is distilled. The fermentation process predominantly consumes the starch in cereal grains, and other nutrients such as protein are thereby concentrated. Alcohol fermentation of corn is done commercially, and a small amount of wheat is also used for this purpose. Fractionation and characterization of corn stillage and of corn distillers' dried grains with and without solubles were reported previously (Wu et al 1981, Wu and Stringfellow 1982). Satterlee et al (1976) prepared protein concentrate from fermented corn and fermented wheat by extraction with alkali. Tsen et al (1982, 1983) incorporated distillers' dried grain flours in bread and cookies. Prentice (1978) and Prentice et al (1978) blended brewers' spent grain with flour for muffin and cookie formulations. Finley and Hanamoto (1980) incorporated various fractions of brewers' spent grains into bread. Some composition data on wheat distillers' grains and wheat distillers' grains with solubles are available
Impact of Qt Interval Prolongation Following Antiarrhythmic Drug Therapy On Left Ventricular Function (2016)

QT interval prolongation following antiarrhythmic therapy does not affect significantly left ventricular function.
Bitsquatting (2013)

Over the last fifteen years, several types of attacks against domain names and the companies relying on them have been observed. The well-known cybersquatting of domain names gave way to typosquatting, the abuse of a user's mistakes when typing a URL in her browser's address bar. Recently, a new attack against domain names surfaced, namely bitsquatting. In bitsquatting, an attacker leverages random bit-errors occurring in the memory of commodity computers and smartphones, to redirect Internet traffic to attacker-controlled domains.
On the construction of layered space-time coded modulation (STCM) codes employing MTCM code design techniques (2003)

The field of space-time coded modulation (STCM) has recently attracted interest as a means of improving link reliability in mobile environments. It has been shown that these space-time multiple-antenna systems provide very high capacity when compared to single antenna systems in a Rayleigh fading environment. Specifically for the forward link, space-time coded transmit diversity should be incorporated into the wireless communication system in order to take full advantage of the combined space- (multiple-transmit antennas) and time- (forward error correction coding) diversity scheme. In this paper, we adopt an heuristic approach to the design and evaluation of diagonally layered STCM schemes by utilising classical multiple-trellis coded-modulation (MTCM) techniques. We focus mainly on the construction of simple 4-state diagonally layered space-time convolutionally coded systems. The designed STCM systems will be used in a comparative performance investigation with the originally proposed STCM codes. Computer simulations are used to evaluate the performance of the code designs on flat Rayleigh fading channels.
Extraction of rare earths from monazine sand, rare earth residues (2009)

[Abstract not available]