gebhart/patrepeval huggingface dataset:
IL‐1β Increases uPA and uPA Receptor Expression in Human Gingival Fibroblasts (2001)

The binding of urokinase-type plasminogen activator (uPA) to its receptor (uPAR) in various cell types has been proposed as an important feature of many cellular processes requiring extracellular proteolysis, cell adhesion, motility, and invasion. uPAR attaches to the cell surface with a glycosylphophatidylinositol (GPI) anchor, and serves to localize and accelerate the proteolysis cascade. In this study, we examined both uPA and uPAR levels in human gingival fibroblasts treated with an inflammatory cytokine, interleukin-1beta (IL-1beta). PA activity in the cell lysate was increased by treatment with IL-1beta. Further, PA activity released by phosphatidylinositol-specific phospholipase C, which detaches the GPI anchor, was also increased by IL-1beta. The activity was inhibited by amiloride, a specific inhibitor of uPA. In addition, IL-1beta increased the protein and mRNA levels of both uPA and uPAR in gingival fibroblasts. These findings suggest that the enhancement of uPA and uPAR levels by IL-1beta may play an important role in the progression of periodontal diseases through pericellular proteolysis, and subsequent cellular behavior.
A Geophysical Method for Detection and Quantification of Dense Non‐Aqueous Phase Liquids (DNAPL) in the Subsurface (2003)

PreviousNext No AccessSymposium on the Application of Geophysics to Engineering and Environmental Problems 2003A Geophysical Method for Detection and Quantification of Dense Non‐Aqueous Phase Liquids (DNAPL) in the SubsurfaceAuthors: Mark StewartLoren NorthMark StewartGeology Department, USF, Tampa, FL and Loren NorthCDM, Ft. Myers, FLhttps://doi.org/10.4133/1.2923108 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Abstract Introductory paragraph for this paper is available only in the PDF and GZipped PS filesPermalink: https://doi.org/10.4133/1.2923108FiguresReferencesRelatedDetails Symposium on the Application of Geophysics to Engineering and Environmental Problems 2003ISSN (online):1554-8015Copyright: 2003 Pages: 1491 publication data© 2003 Copyright © 2003 The Environmental and Engineering Geophysical SocietyPublisher:Environmental & Engineering Geophysical Society HistoryPublished Online: 30 Sep 2008 CITATION INFORMATION Mark Stewart and Loren North, (2003), "A Geophysical Method for Detection and Quantification of Dense Non‐Aqueous Phase Liquids (DNAPL) in the Subsurface," Symposium on the Application of Geophysics to Engineering and Environmental Problems Proceedings : 1042-1058. https://doi.org/10.4133/1.2923108 Plain-Language Summary PDF DownloadLoading ...
Novel Neuromodulation Approach to Improve Left Ventricular Contractility in Heart Failure (2020)

In this first-in-human study, we demonstrated that in humans with stable heart failure, left ventricular contractility could be accentuated without an increase in heart rate or left ventricular filling pressures. This benign increase in contractility may benefit patients admitted for acute decompensated heart failure.
Complex nonlinear dynamics in the limit of weak coupling of a system of microcantilevers connected by a geometrically nonlinear tunable nanomembrane (2014)

Intentional utilization of geometric nonlinearity in micro/nanomechanical resonators provides a breakthrough to overcome the narrow bandwidth limitation of linear dynamic systems. In past works, implementation of intentional geometric nonlinearity to an otherwise linear nano/micromechanical resonator has been successfully achieved by local modification of the system through nonlinear attachments of nanoscale size, such as nanotubes and nanowires. However, the conventional fabrication method involving manual integration of nanoscale components produced a low yield rate in these systems. In the present work, we employed a transfer-printing assembly technique to reliably integrate a silicon nanomembrane as a nonlinear coupling component onto a linear dynamic system with two discrete microcantilevers. The dynamics of the developed system was modeled analytically and investigated experimentally as the coupling strength was finely tuned via FIB post-processing. The transition from the linear to the nonlinear dynamic regime with gradual change in the coupling strength was experimentally studied. In addition, we observed for the weakly coupled system that oscillation was asynchronous in the vicinity of the resonance, thus exhibiting a nonlinear complex mode. We conjectured that the emergence of this nonlinear complex mode could be attributed to the nonlinear damping arising from the attached nanomembrane.
A Systematic Evaluation of the Bag-of-Frames Representation for Music Information Retrieval (2014)

There has been an increasing attention on learning feature representations from the complex, high-dimensional audio data applied in various music information retrieval (MIR) problems. Unsupervised feature learning techniques, such as sparse coding and deep belief networks have been utilized to represent music information as a term-document structure comprising of elementary audio codewords. Despite the widespread use of such bag-of-frames (BoF) model, few attempts have been made to systematically compare different component settings. Moreover, whether techniques developed in the text retrieval community are applicable to audio codewords is poorly understood. To further our understanding of the BoF model, we present in this paper a comprehensive evaluation that compares a large number of BoF variants on three different MIR tasks, by considering different ways of low-level feature representation, codebook construction, codeword assignment, segment-level and song-level feature pooling, tf-idf term weighting, power normalization, and dimension reduction. Our evaluations lead to the following findings: 1) modeling music information by two levels of abstraction improves the result for difficult tasks such as predominant instrument recognition, 2) tf-idf weighting and power normalization improve system performance in general, 3) topic modeling methods such as latent Dirichlet allocation does not work for audio codewords.
Oxygen Pressure as Biosensor for Rate Adaptive Cardiac Pacing (1994)

A new carbon based oxygen sensor for rate adaptive cardiac pacing has been developed. It measures oxygen partial pressure In mixed venous blood. The system was implanted in six sheep with a mean follow-up time of 261 days after implant. The sensor reacted in a physiological manner when the animals were at rest as well as during exercise. Long-term sensor stability and sensitivity were satisfactory, and we found excellent correlation with spontaneous heart rate variations and with oxygen saturation measurements. The sensor shows great promise although our experience is still limited and further development on the concept is on-going.
Random Numbers Generated from Audio and Video Sources (2013)

Random numbers are very useful in simulation, chaos theory, game theory, information theory, pattern recognition, probability theory, quantum mechanics, statistics, and statistical mechanics. The random numbers are especially helpful in cryptography. In this work, the proposed random number generators come from white noise of audio and video (A/V) sources which are extracted from high-resolution IPCAM, WEBCAM, and MPEG-1 video files. The proposed generator applied on video sources from IPCAM and WEBCAM with microphone would be the true random number generator and the pseudorandom number generator when applied on video sources from MPEG-1 video file. In addition, when applying NIST SP 800-22 Rev.1a 15 statistics tests on the random numbers generated from the proposed generator, around 98% random numbers can pass 15 statistical tests. Furthermore, the audio and video sources can be found easily; hence, the proposed generator is a qualified, convenient, and efficient random number generator.
Molecular targets of CD45 in B cell antigen receptor signal transduction (1997)

Expression of the phosphotyrosine phosphatase CD45 is essential for B cell Ag receptor (BCR)-mediated p21ras activation and calcium mobilization. To examine the molecular basis of this requirement, we analyzed signaling events following BCR ligation in CD45-deficient (CD45-) and CD45-reconstituted (CD45+) variants of J558Lmicrom3 cells. Ag stimulation resulted in tyrosine phosphorylation of cellular proteins in both cells. However, the spectrum of proteins phosphorylated in the CD45+ cells was qualitatively and/or quantitatively distinct from that in the CD45- cells. Among the protein tyrosine kinases examined, the Src family kinases Fyn and Blk were inducibly tyrosine phosphorylated and activated by receptor ligation only in CD45+ cells. While Ag-induced Btk tyrosine phosphorylation occurred in both cells, its activation was greatly diminished in the CD45- cells. Analysis of specific effector molecules revealed that tyrosine phosphorylation of Shc, but not rasGAP or Vav, correlated with the unique ability of BCR ligation to trigger p21ras activation in CD45+ cells. BCR-mediated Shc phosphorylation and recruitment of Grb2 depended on CD45 expression. Thus, Shc tyrosine phosphorylation may be the primary CD45-dependent mechanism by which Ag receptors are coupled to the p21ras pathway in J558Lmicrom3. In addition, phospholipase Cgamma1 (PLCgamma1) and PLCgamma2 were tyrosine phosphorylated upon Ag stimulation in CD45- cells, despite much reduced inositol trisphosphate production and lack of calcium mobilization. These findings suggest that CD45 may modulate events other than PLCgamma phosphorylation, which regulate phosphoinositide hydrolysis and the calcium mobilization response following BCR ligation.
Novel Aromatic Isothiouronium Derivatives Which Act as High Affinity Competitive Antagonists of Alkali Metal Cations on Na/K-ATPase (1995)

This paper describes properties of a novel family of aromatic isothiouronium derivatives, which act as Na(+)-like competitive antagonists on renal Na/K-ATPase. The derivatives are reversible competitors of Rb+ and Na+ occlusion. Ki values of the most potent compounds, 1-bromo-2,4,6-tris(methylisothiouronium)benzene (Br-TITU) and 1,3-dibromo-2,4,6-tris(methylisothiouronium)benzene(Br2-TITU ), 0.65 and 0.32 microM, respectively, are 15-30-fold lower than Ki values of the bis-guanidinium derivatives described previously (David, P., Mayan, H., Cohen, H., Tal, D. M., and Karlish, S. J. D. (1992) J. Biol. Chem. 267, 1141-1149), and represent the lowest reported values for cation antagonists. Using fluorescein-labeled Na/K-ATPase, all derivatives have been shown to stabilize the E1 conformation when bound at high affinity sites (i.e. they are sodium-like). In addition, in one condition (10 mM Tris-HCl, pH 8.1), high concentrations of Br-TITU (KD approximately 10 microM) appear to stabilize an E2 conformation. We propose a model which allows for simultaneous binding of the antagonists to high affinity cytoplasmic sites and low affinity sites, which may be at the extracellular surface. Blockage of cation occlusion by the isothiouronium derivatives at the cytoplasmic surface probably occurs at the entrance to the occlusion sites, which is recognized both by Na+ antagonists and by Na+ or K+ ions. Unlike the alkali metal cations, the Na+ antagonists are not occluded or transported (see also Or, E., David, P., Shainskaya, A., Tal, D. M., and Karlish, S. J. D. (1993) J. Biol. Chem. 268, 16929-16937). The isothiouronium derivatives appear to be promising candidates for further development as affinity labels of cation binding domains, for kinetic analysis of isoforms or mutated Na/K pumps, or as probes of other cation transport proteins.
Crosslinking CD4 by human immunodeficiency virus gp120 primes T cells for activation-induced apoptosis. (1992)

During human immunodeficiency virus (HIV) infection there is a profound and selective decrease in the CD4+ population of T lymphocytes. The mechanism of this depletion is not understood, as only a small fraction of all CD4+ cells appear to be productively infected with HIV-1 in seropositive individuals. In the present study, crosslinking of bound gp120 on human CD4+ T cells followed by signaling through the T cell receptor for antigen was found to result in activation-dependent cell death by a form of cell suicide termed apoptosis, or programmed cell death. The data indicate that even picomolar concentrations of gp120 prime T cells for activation-induced cell death, suggesting a mechanism for CD4+ T cell depletion in acquired immune deficiency syndrome (AIDS), particularly in the face of concurrent infection and antigenic challenge with other organisms. These results also provide an explanation for the enhancement of infection by certain antibodies against HIV, and for the paradox that HIV appears to cause AIDS after the onset of antiviral immunity.
Antitumor Efficacy of Anti-GD2 IgG1 Is Enhanced by Fc Glyco-Engineering (2016)

The affinity of therapeutic antibodies for Fcγ receptors (FcγRs) strongly influences their antitumor potency. To generate antibodies with optimal binding and immunologic efficacy, we compared the affinities of different versions of an IgG1 Fc region that had an altered peptide backbone, altered glycans, or both. To produce IgG1 with glycans that lacked α1,6-fucose, we used CHO cells that were deficient in the enzyme UDP-N-acetylglucosamine: α-3-d-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT1), encoded by the MGAT1 gene. Mature N-linked glycans require this enzyme, and without it, CHO cells synthesize antibodies carrying only Man5-GlcNAc2, which were more effective in antibody-dependent cell-mediated cytotoxicity (ADCC). Our engineered IgG1, hu3F8-IgG1, is specific for GD2, a neuroendocrine tumor ganglioside. Its peptide mutant is IgG1-DEL (S239D/I332E/A330L), both produced in wild-type CHO cells. When produced in GnT1-deficient CHO cells, we refer to them as IgG1n and IgG1n-DEL, respectively. Affinities for human FcγRs were measured using Biacore T-100 (on CD16 and CD32 polymorphic alleles), their immunologic properties compared for ADCC and complement-mediated cytotoxicity (CMC) in vitro, and pharmacokinetics and antitumor effects were compared in vivo in humanized mice. IgG1n and IgG1n-DEL contained only mannose and acetylglucosamine and had preferential affinity for activating CD16s, over inhibitory CD32B, receptors. In vivo, the antitumor effects of IgG1, IgG1-DEL, and IgG1n-DEL were similar but modest, whereas IgG1n was significantly more effective (P < 0.05). Thus, IgG1n antibodies produced in GnT1-deficient CHO cells may have potential as improved anticancer therapeutics. Cancer Immunol Res; 4(7); 631-8. ©2016 AACR.
Multivariable control of a compact disc player using DSPs (2005)

This paper considers robust multivariable control of a compact disc mechanism using digital signal processors. The design problem is to achieve good track-following and focusing performance in the presence of disturbances and norm-bounded structured plant uncertainty. This robust performance problem has been solved in the /spl mu/-framework using the D-K-iteration scheme. After controller order reduction, the /spl mu/-based controller has been implemented using two parallel digital signal processors.
The Molecular Basis for the Treatment of Sickle Cell Crisis by Intravenous Urea. (1970)

Meeting Abstracts1 May 1970The Molecular Basis for the Treatment of Sickle Cell Crisis by Intravenous Urea.Robert M. Nalbandian, M.D., Raymond Henry, Ph.D., Bruce Nichols, B.A., Dale L. Kessler, M.D., Frank R. Camp Jr., Keats K. Vining Jr., M.D., (Associate)Robert M. Nalbandian, M.D.Search for more papers by this author, Raymond Henry, Ph.D.Search for more papers by this author, Bruce Nichols, B.A.Search for more papers by this author, Dale L. Kessler, M.D.Search for more papers by this author, Frank R. Camp Jr.Search for more papers by this author, Keats K. Vining Jr., M.D., (Associate)Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-72-5-795_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptWe show that the molecular basis for the use of intravenous urea in the treatment of sickle cell crisis is supported by molecular pathology and in vitro experiments. We have been able to inhibit and to reverse sickling in both heterozygous and homozygous S intact erythrocytes without hemolysis by using urea in an osmotically balanced invert sugar solution. Also, concentrated hemolysates of both heterozygous and homozygous hemoglobin S have been prevented from gelling at 37 C with the use of urea, indicating inhibition of sickling. The molecular basis for the effectiveness of urea in blocking and reversing sickling is implicit... This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAuthors: Robert M. Nalbandian, M.D.; Raymond Henry, Ph.D.; Bruce Nichols, B.A.; Dale L. Kessler, M.D.; Frank R. CampJr.; Keats K. ViningJr., M.D., (Associate)Affiliations: Grand Rapids, Mich. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics Cited byThe unexpected pathway to the creation of the HbA1c test and the discovery of AGE’sDesign and evaluation of a sickle cell anemia projects laboratory for freshmen medical studentsThe interactions of sickle hemoglobinModified Sickledex tube test: a specific test for S hemoglobin 1 May 1970Volume 72, Issue 5Page: 795-795KeywordsHemoglobinMolecular pathologyUrea ePublished: 1 December 2008 Issue Published: 1 May 1970 PDF downloadLoading ...
Leukotriene B4 mediates γδ T lymphocyte migration in response to diverse stimuli (2009)

Herein, we investigated the involvement of the 5-LO-derived lipid mediator LTB(4) in gammadelta T cell migration. When injected into the i.pl. space of C57BL/6 mice, LTB(4) triggered gammadelta T lymphocyte mobilization in vivo, a phenomenon also observed in in vitro chemotaxis assays. The i.pl. injection of Escherichia coli endotoxin (LPS) triggered increased levels of LTB(4) in pleural cavities. The in vivo inhibition of LTB(4) biosynthesis by the 5-LO inhibitor zileuton or the FLAP inhibitor MK886 attenuated LPS-induced gammadelta T cell accumulation into pleural cavities. Accordingly, 5-LO KO mice failed to recruit gammadelta T cells into the inflammatory site after i.pl. LPS. Antagonists of the high-affinity LTB(4) receptor BLT1, CP105,696, and LY292476 also attenuated LPS-induced gammadelta T cell accumulation in pleural cavities as well as in vitro chemotaxis toward pleural washes obtained from LPS-simulated mice. LTB(4)/BLT1 also accounted for gammadelta T cell migration induced by i.pl. administration of Mycobacterium bovis BCG or antigen in sensitized mice. BLT1 was expressed on naïve, resident as well as LPS-recruited gammadelta T cells. Isolated gammadelta T cells were found to undergo F-actin cytoskeleton reorganization when incubated with LTB(4) in vitro, confirming that gammadelta T lymphocytes can respond directly to LTB(4). In addition to its direct effect on gammadelta T cells, LTB(4) triggered their accumulation indirectly, via modulation of CCL2 production in mouse pleural cavities. These data show that gammadelta T cell migration into the pleural cavity of mice during diverse inflammatory responses is dependent on LTB(4)/BLT1.
Two improved methods for preparing ferritin-protein conjugates for electron microscopy. (1975)

Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin-antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells.
Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents (1981)

Changes in the membrane potential, pH gradient, proton motive force, and intracellular pH of Escherichia coli were followed during the chemotactic responses to a variety of potentially membrane-active compounds. Lipophilic weak acids, decreases in extracellular pH, and nigericin each caused a repellent response. Lipophilic weak bases, increases in extracellular pH, and valinomycin in the presence of K+ each caused an attractant response. Changes in membrane potential, pH gradient, and proton motive force did not correlate with the behavioral responses to these treatments, but changes in intracellular pH did correlate. Furthermore, the strength of the response to a weak acid was correlated with the magnitude of the change of the intracellular pH, and many compounds which could alter the intracellular pH were found to be chemotactically active. Apparently these attractants and repellents are not detected by specific chemoreceptors but rather are detected via the ability of cells to sense and respond to changes in intracellular pH. The pathway of sensory transduction which proceeds through methyl-accepting chemotaxis protein I was found to be involved in the response to a change in intracellular pH.
RF module using MCM-L and BGA technology for 5 GHz WLAN application (2003)

This paper presents the design concept and experimental results about small size radio frequency (RF) module for 5 GHz wireless local area network (WLAN). Functions of the RF module include from base band input/output to antenna switch. The size is 20 mm/spl times/15 mm/spl times/3 mm and that is minimum size in modules with same functions as far as the authors get to know. For the purpose of small size and low cost, generally known technologies are used. They are multi chip module on laminated-base dielectric substrate (MCM-L) technology, interconnection of each layer using build-up technology, flip chip technology and ball grid array (BGA) technology. Parts and ICs are on both sides of the RF module's substrate and are vertically connected in the substrate. In order to ensure electrical performance at 5 GHz frequency band in the high-density package, transmission line characteristics in the substrate and BGA characteristic are simulated by 3 dimensional (3D) electromagnetic (EM) software. The fabricated RF module is evaluated using the test set up for 5 GHz WLAN application and the result is shown.
The Effect of Wire Pens, Floor Pens and Cages on Bone Characteristics of Laying Hens (1970)

SEVERAL factors which may lead to osteoporosis or bone fragility include a deficiency of sex hormones, calcium deficiency, malnutrition, or a lack of normal stimulation due to stress and/or strain such as encountered by prolonged recumbency, plaster casts, etc. (Jackson, 1967). It has been well established by Trueta (1968) that immobilization, bed rest, and lack of muscular activity are all factors contributing to osteoporosis in humans. It was also demonstrated that bone rarefaction occurred in rabbits whose feet and legs were immobilized by plaster casts. It has been demonstrated (Rowland et al., 1968) that hens maintained in cages had a lower bone breaking strength and a lower tibia ash than those kept in floor pens. It is possible that hens kept in cages develop osteoporosis or bone fragility more rapidly than floor birds due to a lack of exercise. Therefore, these studies were conducted to determine if exercise could be…