gebhart/patrepeval huggingface dataset:
Novel Infectivity-Enhanced Oncolytic Adenovirus with a Capsid-Incorporated Dual-Imaging Moiety for Monitoring Virotherapy in Ovarian Cancer (2009)

We sought to develop a cancer-targeted, infectivity-enhanced oncolytic adenovirus that embodies a capsid-labeling fusion for noninvasive dual-modality imaging of ovarian cancer virotherapy. A functional fusion protein composed of fluorescent and nuclear imaging tags was genetically incorporated into the capsid of an infectivity-enhanced conditionally replicative adenovirus. Incorporation of herpes simplex virus thymidine kinase (HSV-tk) and monomeric red fluorescent protein 1 (mRFP1) into the viral capsid and its genomic stability were verified by molecular analyses. Replication and oncolysis were evaluated in ovarian cancer cells. Fusion functionality was confirmed by in vitro gamma camera and fluorescent microscopy imaging. Comparison of tk-mRFP virus to single-modality controls revealed similar replication efficiency and oncolytic potency. Molecular fusion did not abolish enzymatic activity of HSV-tk as the virus effectively phosphorylated thymidine both ex vivo and in vitro. In vitro fluorescence imaging demonstrated a strong correlation between the intensity of fluorescent signal and cytopathic effect in infected ovarian cancer cells, suggesting that fluorescence can be used to monitor viral replication. We have in vitro validated a new infectivity-enhanced oncolytic adenovirus with a dual-imaging modality-labeled capsid, optimized for ovarian cancer virotherapy. The new agent could provide incremental gains toward climbing the barriers for achieving conditionally replicated adenovirus efficacy in human trials.
Yersinia pseudotuberculosis disrupts intestinal barrier integrity through hematopoietic TLR-2 signaling (2012)

Intestinal barrier function requires intricate cooperation between intestinal epithelial cells and immune cells. Enteropathogens are able to invade the intestinal lymphoid tissue known as Peyer's patches (PPs) and disrupt the integrity of the intestinal barrier. However, the underlying molecular mechanisms of this process are poorly understood. In mice infected with Yersinia pseudotuberculosis, we found that PP barrier dysfunction is dependent on the Yersinia virulence plasmid and the expression of TLR-2 by hematopoietic cells, but not by intestinal epithelial cells. Upon TLR-2 stimulation, Y. pseudotuberculosis-infected monocytes activated caspase-1 and produced IL-1β. In turn, IL-1β increased NF-κB and myosin light chain kinase activation in intestinal epithelial cells, thus disrupting the intestinal barrier by opening the tight junctions. Therefore, Y. pseudotuberculosis subverts intestinal barrier function by altering the interplay between immune and epithelial cells during infection.
Cloning and expression in Escherichia coli of a yeast mannosyltransferase from the asparagine-linked glycosylation pathway. (1984)

The yeast Saccharomyces cerevisiae temperature-sensitive lethal mutant alg1-1, has been previously shown to lack the activity necessary for the addition of the first mannose residue in the synthesis of lipid-linked precursor oligosaccharide. The gene ALG1 has been cloned by complementation of the temperature-sensitive mutation alg1-1 with a total genomic DNA library. The original DNA fragment isolated was 11,300 base pairs and has been subcloned to a 1,500-base pair fragment which is still capable of complementing alg1-1. The gene ALG1 has been mapped on chromosome II at a distance of 2.1 map units from LYS2. The ALG1 gene product has been shown to catalyze the transfer of a mannosyl residue from GDP-mannose to the lipid-linked acceptor GlcNAc2, yielding Man beta 1-4GlcNAc2-lipid, in lysates from Escherichia coli transformants. This result proves that ALG1 is the structural gene for the first mannosyltransferase in lipid-linked oligosaccharide assembly.
Adsorption Kinetics and Equilibria of Carbon Dioxide, Ethylene, and Ethane on 4A(CECA) Zeolite (2010)

The adsorption kinetics and equilibria of pure carbon dioxide (CO2), ethylene (C2H4), and ethane (C2H6) on 4A(CECA) commercial zeolite have been measured over the temperature range T = (293.15 to 353.15) K using a glass-vacuum volumetric device. The adsorption rates of the gases were measured automatically via a custom acquisitions data card that was capable of registering pressure and time (t) data five times per second in the first period for t = (0 to 180) s, simultaneously. All of the gases showed a decreasing adsorption affinity and isosteric heat of adsorption in the order CO2 > C2H4 > C2H6. Unlike CO2, an activated diffusion for C2H4 and C2H6 for low t was observed. The adsorption activation energy for ethane Ea = (14.6 ± 0.2) kJ·mol−1 was found to be slightly higher than that for ethylene Ea = (13.8 ± 1) kJ·mol−1. The dual Langmuir model described the CO2 adsorption isotherms, whereas those for C2H4 and C2H6 were fitted with the Sips equation at (293.15 and 323.15) K. The three gases were reversibly adsorbed, and the adsorption selectivity for gas binary mixtures decreased in the sequence CO2/C2H6 > C2H4/C2H6 > CO2/C2H4. The obtained results indicate that 4A(CECA) zeolite could be a good candidate for separating these binary gas mixtures at 293.15 K.
Mucin Production by Staphylococcus epidermidis (1986)

The production of an exopolysaccharide (mucin) by some Staphylococcus epidermidis strains facilitates bacterial adhesion to prosthetic vascular grafts and may play an important role in adherence-mediated growth. An in vitro model was developed to measure the differential adherence of mucin-producing (RP-12) and nonmucin-producing (SP-2) S epidermidis strains onto expanded polytef and velour knitted Dacron graft material. After incubation in a 10(7)/mL suspension of organisms, graft specimens were repeatedly washed to remove nonadherent organisms and then sonicated to dislodge adherent organisms. Bacterial adherence was calculated from a quantitative culture of the sonication effluent. Both S epidermidis strains adhered in ten to 100 times greater numbers to the knitted Dacron graft material than to expanded polytef. The production of an exopolysaccharide by the RP-12 strain significantly increased adherence to both graft types compared with the SP-2 strain. The increased adherence of the RP-12 strain was inhibited by adding D-mannosamine to the inoculum. The in vitro model developed is well suited for further study of the mechanisms by which bacteria adhere to and colonize vascular grafts.
Monitoring System Analysis for Evaluating a Building’s Envelope Energy Performance through Estimation of Its Heat Loss Coefficient (2018)

The present article investigates the question of building energy monitoring systems used for data collection to estimate the Heat Loss Coefficient (HLC) with existing methods, in order to determine the Thermal Envelope Performance (TEP) of a building. The data requirements of HLC estimation methods are related to commonly used methods for fault detection, calibration, and supervision of energy monitoring systems in buildings. Based on an extended review of experimental tests to estimate the HLC undertaken since 1978, qualitative and quantitative analyses of the Monitoring and Controlling System (MCS) specifications have been carried out. The results show that no Fault Detection and Diagnosis (FDD) methods have been implemented in the reviewed literature. Furthermore, it was not possible to identify a trend of technology type used in sensors, hardware, software, and communication protocols, because a high percentage of the reviewed experimental tests do not specify the model, technical characteristics, or selection criteria of the implemented MCSs. Although most actual Building Automation Systems (BAS) may measure the required parameters, further research is still needed to ensure that these data are accurate enough to rigorously apply HLC estimation methods.
2-Amino-3-phenyl-1,1,1-trifluoropropanes. Fluorine analogs of amphetamines (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT2-Amino-3-phenyl-1,1,1-trifluoropropanes. Fluorine analogs of amphetaminesRoger M. Pinder, Roger W. Brimblecombe, and David Morris GreenCite this: J. Med. Chem. 1969, 12, 2, 322–324Publication Date (Print):March 1, 1969Publication History Published online1 May 2002Published inissue 1 March 1969https://doi.org/10.1021/jm00302a031RIGHTS & PERMISSIONSArticle Views117Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (412 KB) Get e-Alerts Get e-Alerts
A furostanol glycoside from garlic, bulbs of Allium sativum L. (1988)

A new furostanol glycoside (1) was isolated from garlic, bulbs of Allium sativum L. (Liliaceae), and the structure was established to be 26-O-β-glucopyranosyl 22-hydroxy-25(R)-5α-furostane-3β, 6β, 26-triol 3-O-β-glucopyranosyl(1→2)-[β-glucopyranosyl(1→3)]-β-glucopyranosyl-(1→4)-β-galactopyranoside, i.e., proto-eruboside-B. Eruboside-B (3) obtained by enzymatic hydrolysis of 1 inhibited the growth of Candida albicans in vitro (25 μg/ml MIC).
Atomic configuration of the Er-O luminescence center in Er-doped GaAs with oxygen codoping (1994)

This article investigates the structure of an Er luminescence center in GaAs by using its intra-4f-shell luminescence spectrum as an atomic probe to identify the atomic configuration. This Er center is formed in GaAs by metalorganic chemical vapor deposition with O codoping and the center shows a high efficiency and a sharp luminescence spectrum under above-band-gap photoexcitation. A single luminescence line in the spectrum of a GaAs:Er,O splits into more than eight lines in the spectrum of Al0.01Ga0.99As:Er,O. This drastic change due to the addition of 1% Al can be explained by assuming that, because of the high tendency of Al atoms to react with O atoms, the Al atoms preferentially occupy the nearest-neighbor Ga sites of two O atoms, both of which are coupled with the Er atom. Based on the luminescence spectra and additional experiments of Rutherford backscattering and secondary ion mass spectroscopy, we propose that the structure of the Er luminescence center under study is Er occupying the Ga sublattice with two O atoms most likely located at the nearest-neighbor As sites. An Er-related spectrum of GaAs0.97P0.03:Er,O can also be understood based on this model if the chemical difference of Al and P is taken into account.
A New Correction Factor for Theoretical Prediction of Mass Transfer Coefficients in Bubble Columns (2006)

The volumetric liquid-side mass transfer coefficients kLa in 1-butanol, toluene, ethanol and tap water were used in order to show that, when the classical penetration theory is applied to ellipsoidal bubbles, it needs to be corrected. The kLa data were obtained in a 0.102 m diameter bubble column operated in the homogeneous flow regime at pressures varied from 0.1 to 1.0 MPa. The new correction factor (lower than unity) was correlated with the dimensionless Eötvös number, Eo (gravitational force to surface tension force ratio). The kLa data (32 points) are fitted with a mean error of 8.2%.
Reflection and transmission behaviour of building materials at 60 GHz (2002)

Mobile radio communications at very high data rates require wide bandwidths which are only available at very high frequency bands. In order to model millimetre wave radio scenarios profound knowledge of the reflection and transmission properties of building materials is required. For a variety of typical smooth and rough building materials reflection scattering, bistatic reflection and transmission measurements have been carried out at 50 GHz. This document introduces briefly the basic physical principles of reflection and transmission as well as the used measuring technique. The measurement results are presented graphically and in tabular form. Additionally, the comparison of measured and calculated profiles is given, using a heuristic approach for permittivity estimation.
Normative Influences on Aggression in Urban Elementary School Classrooms (2000)

We report a study aimed at understanding the effects of classroom normative influences on individual aggressive behavior, using samples of 614 and 427 urban elementary school children. Participants were assessed with measures of aggressive behavior and normative beliefs about aggression. We tested hypotheses related to the effects of personal normative beliefs, descriptive classroom norms (the central tendency of classmates' aggressive behavior), injunctive classroom normative beliefs (classmates' beliefs about the acceptability of aggression), and norm salience (student and teacher sanctions against aggression) on longitudinal changes in aggressive behavior and beliefs. injunctive norms affected individual normative beliefs and aggression, but descriptive norms had no effect on either. In classrooms where students and teachers made norms against aggression salient, aggressive behavior diminished over time. Implications for classroom behavior management and further research are discussed.
Near-Field Radiative Cooling of Nanostructures (2012)

We measure near-field radiative cooling of a thermally isolated nanostructure up to a few degrees and show that in principle this process can efficiently cool down localized hotspots by tens of degrees at submicrometer gaps. This process of cooling is achieved without any physical contact, in contrast to heat transfer through conduction, thus enabling novel cooling capabilities. We show that the measured trend of radiative cooling agrees well theoretical predictions and is limited mainly by the geometry of the probe used here as well as the minimum separation that could be achieved in our setup. These results also pave the way for realizing other new effects based on resonant heat transfer, like thermal rectification and negative thermal conductance.
Interrogation of fiber Bragg-grating resonators by polarization-spectroscopy laser-frequency locking (2007)

We report on an optically-based technique that provides an efficient way to track static and dynamic strain by locking the frequency of a diode laser to a fiber Bragg-grating Fabry-Pérot cavity. For this purpose, a suitable optical frequency discriminator is generated exploiting the fiber natural birefringence and that resulting from the gratings inscription process. In our scheme, a polarization analyzer detects dispersive-shaped signals centered on the cavity resonances without need for additional optical elements in the resonator or any laser-modulation technique. This method prevents degradation of the resonator quality and maintains the configuration relatively simple, demonstrating static and dynamic mechanical sensing below the picostrain level.
Role of reactive oxygen species in contraction‐mediated glucose transport in mouse skeletal muscle (2006)

Exercise increases glucose transport into skeletal muscle via a pathway that is poorly understood. We investigated the role of endogenously produced reactive oxygen species (ROS) in contraction-mediated glucose transport. Repeated contractions increased 2-deoxyglucose (2-DG) uptake roughly threefold in isolated, mouse extensor digitorum longus (fast-twitch) muscle. N-Acetylcysteine (NAC), a non-specific antioxidant, inhibited contraction-mediated 2-DG uptake by approximately 50% (P < 0.05 versus control values), but did not significantly affect basal 2-DG uptake or the uptake induced by insulin, hypoxia or 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR, which mimics AMP-mediated activation of AMP-activated protein kinase, AMPK). Ebselen, a glutathione peroxidase mimetic, also inhibited contraction-mediated 2-DG uptake (by almost 60%, P < 0.001 versus control values). Muscles from mice overexpressing Mn2+-dependent superoxide dismutase, which catalyses H2O2 production from superoxide anions, exhibited a approximately 25% higher rate of contraction-mediated 2-DG uptake versus muscles from wild-type control mice (P < 0.05). Exogenous H2O2 induced oxidative stress, as judged by an increase in the [GSSG]/[GSH + GSSG] (reduced glutathione + oxidized glutathione) ratio to 2.5 times control values, and this increase was substantially blocked by NAC. Similarly, NAC significantly attenuated contraction-mediated oxidative stress as judged by measurements of glutathione status and the intracellular ROS level with the fluorescent indicator 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein (P < 0.05). Finally, contraction increased AMPK activity and phosphorylation approximately 10-fold, and NAC blocked approximately 50% of these changes. These data indicate that endogenously produced ROS, possibly H2O2 or its derivatives, play an important role in contraction-mediated activation of glucose transport in fast-twitch muscle.
Force-frequency effect on the Q-factor of thickness- shear mode quartz and langasite resonator at high temperatures (2011)

A theoretical study was conducted for a 20 MHz circular resonator to investigate the force-frequency effect on the Q-factor value for the slow (C-) and fast (B-) thickness-shear mode using the 3-D Lagrangian finite-element model (FEM), and the Sinha-Tiersten perturbation method. The calculations were carried out for doubly rotated quartz cuts and single rotated langasite (LGS) cut. Based on the frequency spectrum analysis, the best aspect ratio for each crystal cut angle was investigated for the force-frequency effect at 25 and 300°C. The Q-factor calculations are based on the intrinsic viscosity of crystalline quartz and LGS. This paper provides the foremost computational result for the force-frequency constants and the Q-factor at 300°C for doubly rotated quartz cuts, and single rotated LGS cut.
Fundamentals of Fluid Film Lubrication (2004)

1: Introduction 2: Bearing Classification and Selection 3: Surface Topography 4: Lubricant Properties 5: Bearing Materials 6: Viscous Flow 7: Reynolds Equation 8: Hydrodynamic Thrust Bearings - Analytical Solutions 9: Hydrodynamic Thrust Bearings - Numerical Solutions 10: Hydrodynamic Journal Bearings - Analytical Solutions 11: Dynamically Loaded Journal Bearings 12: Hydrodynamic Journal Bearings - Numerical Solutions 13: Hydrodynamic Squeeze Film Bearings 14: Hydrostatic Lubrication 15: Hydrodynamic Bearings - Considering Fluid Inertia 16: Gas-Lubricated Thrust Bearings 17: Gas-Lubricated Journal Bearings 18: Hydrodynamic Lubrication of Nonconformal Surfaces 19: Simplified Solutions for Stresses and Deformations 20: General Solution for Stresses and Deformations in Dry Contacts 21: Elastohydrodynamic Lubrication of Rectangular Conjunctions 22: Elastohydrodynamic Lubrication of Ellipitcal Conjunctions 23: Film Thicknesses for Different Regimes of Fluid Film Lubrication 24: Rolling-Element Bearings 25: Additional Elastohydrodynamic Lubrication Applications 26: Non-Newtonian Fluid Effects in Elastohydrodynamic Lubrication 27: Thermo Elastohydrodynamic Lubrication.
A Note on the Unsteady Cavity Flow in a Tunnel (1974)

The unsteady internal cavitating flow such as the one observed in a pump or a turbine is studied for a simple two-dimensional model of a base-cavitating wedge in an infinite tunnel and it is shown how the cavitation compliance can be calculated using the linearized free streamline theory. Numerical values are obtained for the limiting case of a free jet. Two important features are: First, the cavitation compliance is found to be of complex form, having additional resistive and reactive terms beyond the purely inertial oscillation of the whole channel in “slug flow.” Second, the compliance has a strong dependence on frequency.
L'enrichissement isotopique de l'uranium en 1979 (1979)

L'auteur décrit l'usine d'enrichissement de l'uranium d'Eurodif, construite sur le site du Tricastin et, après avoir présenté les installations de séparation isotopique de l'uranium en fonctionnement à l'étranger, dégage les caractéristiques essentielles du marché international de l'enrichissement.
Action-mode of Antimicrobial Altersolanol A in<i>Pseudomonas aeruginosa</i> (1992)

The antimicrobial mechanism of altersolanol A was studied by using Pseudomonas aeruginosa IFO 3080. This reduced anthraquinone antibiotic inhibited the incorporation of radioactive precursors into macromolecules (DNA, RNA, and Protein) in whole cells nonspecifically. It increased respiration of P. aeruginosa cells, though it had no effect on proton condution. Oxidation of NADH in the membrane fraction isolated from P. aeruginosa was enhanced by altersolanol A. Furthermore, altersolanol A stimulated the oxidation of NADH by the enzyme preparation of cytochrome c reductase in the absence of cytochrome c. Altersolanol A seemed to interfere with the respiratory chain in the bacterial membrane as an electron acceptor.
Comparison of Supervised Exercise With and Without Manual Physical Therapy for Patients With Shoulder Impingement Syndrome (2000)

Manual physical therapy applied by experienced physical therapists combined with supervised exercise in a brief clinical trial is better than exercise alone for increasing strength, decreasing pain, and improving function in patients with shoulder impingement syndrome.
Structural distortion and ferroelectric properties of SrBi2(Ta1−xNbx)2O9 (2000)

Ferroelectric materials of the SrBi2(Ta1−xNbx)2O9 solid-solution system were synthesized, and their structural and ferroelectric properties were investigated. Atomic displacements of the ions in the (Ta,Nb)O6 octahedron significantly increase as x increases, which leads to more structural distortion of the perovskite-type unit. The Bi2O2 layer, in contrast, is less distorted in SrBi2Nb2O9 than in SrBi2Ta2O9. The contribution of the perovskite-type unit to total ferroelectric polarization is greater in the SrBi2Nb2O9 sample, while that of the Bi2O2 layer is less; consequently, the total calculated polarization slightly increases. The ferroelectric Curie temperature also increases from 300 (SrBi2Ta2O9) to 440 °C (SrBi2Nb2O9). Three short (Ta,Nb)–O bonds in the (Ta,Nb)O6 octahedron, whose lengths are less than 2 Å, have a covalent character, and the substitution of Nb for Ta makes the bonds more covalent. The strong covalent interaction of the (Ta,Nb)–O bonds increases the structural distortion, resulting in the higher ferroelectric Curie temperature and the larger contribution of the perovskite-type unit to the total spontaneous ferroelectric polarization.
Radiolabeled Chemokine Binding Assays (2003)

Knowledge of a receptor’ pharmacology provides information which is often essential to unraveling the biological function of that receptor. The chemokine system represents a striking example as most receptors in the family bind multiple chemokines, often with complex overlapping selectivities. Two basic methodologies are used to characterize the binding properties of a receptor. In saturation protocols, the binding of a given ligand, usually labeled to allow detection, is determined over a range of concentrations appropriate for that ligand. Competition experiments measure the ability of varying concentrations of an unlabeled ligand to inhibit the binding of a fixed concentration of a labeled ligand. Although saturation protocols have the advantage of theoretical simplicity, they have three significant disadvantages: