gebhart/patrepeval huggingface dataset:
Endothelial Cell-Pericyte Interactions Stimulate Basement Membrane Matrix Assembly: Influence on Vascular Tube Remodeling, Maturation, and Stabilization (2011)

Extracellular matrix synthesis and deposition surrounding the developing vasculature are critical for vessel remodeling and maturation events. Although the basement membrane is an integral structure underlying endothelial cells (ECs), few studies, until recently, have been performed to understand its formation in this context. In this review article, we highlight new data demonstrating a corequirement for ECs and pericytes to properly deposit and assemble vascular basement membranes during morphogenic events. In EC only cultures or under conditions whereby pericyte recruitment is blocked, there is a lack of basement membrane assembly, decreased vessel stability (with increased susceptibility to pro-regressive stimuli), and increased EC tube widths (a marker of dysfunctional EC-pericyte interactions). ECs and pericytes both contribute basement membrane components and, furthermore, both cells induce the expression of particular components as well as integrins that recognize them. The EC-derived factors--platelet derived growth factor-BB and heparin binding-epidermal growth factor--are both critical for pericyte recruitment to EC tubes and concomitant vascular basement membrane formation in vitro and in vivo. Thus, heterotypic EC-pericyte interactions play a fundamental role in vascular basement membrane matrix deposition, a critical tube maturation event that is altered in key disease states such as diabetes and cancer.
Compression and coding for cable-free land acquisition systems (2011)

ABSTRACT The use of cable-free equipment in land acquisition systems is one of the most promising technological breakthroughs toward the minimization of operating costs in high-density surveys. Even if solutions based on remote data storage with some quality data control are reaching a good degree of maturity, their limitations compared to cabled systems are now recognized as unacceptable in land operations. Wireless upload of the entire seismic dataset (or part of it) while shooting is the most challenging task of future generation cable-free systems. Data compression and coding before transmitting over the wireless medium is of strategic importance to reduce the data rate per seismic channel. We have designed data compression systems tailored for cable-free acquisitions. Cable-free receivers are connected by multihop topology so that the encoding of seismic traces optimally exploits the temporal and spatial coherence of data to maximize redundancy. Synthetic and real data are used to simulate the compression settings to evaluate their benefits. Finally, we also discuss the impact of the distortion on signal introduced by the wireless channel fluctuations.
A novel small molecule ameliorates ocular neovascularisation and synergises with anti-VEGF therapy (2016)

Ocular neovascularisation underlies blinding eye diseases such as retinopathy of prematurity, proliferative diabetic retinopathy, and wet age-related macular degeneration. These diseases cause irreversible vision loss, and provide a significant health and economic burden. Biologics targeting vascular endothelial growth factor (VEGF) are the major approach for treatment. However, up to 30% of patients are non-responsive to these drugs and they are associated with ocular and systemic side effects. Therefore, there is a need for small molecule ocular angiogenesis inhibitors to complement existing therapies. We examined the safety and therapeutic potential of SH-11037, a synthetic derivative of the antiangiogenic homoisoflavonoid cremastranone, in models of ocular neovascularisation. SH-11037 dose-dependently suppressed angiogenesis in the choroidal sprouting assay ex vivo and inhibited ocular developmental angiogenesis in zebrafish larvae. Additionally, intravitreal SH-11037 (1 μM) significantly reduced choroidal neovascularisation (CNV) lesion volume in the laser-induced CNV mouse model, comparable to an anti-VEGF antibody. Moreover, SH-11037 synergised with anti-VEGF treatments in vitro and in vivo. Up to 100 μM SH-11037 was not associated with signs of ocular toxicity and did not interfere with retinal function or pre-existing retinal vasculature. SH-11037 is thus a safe and effective treatment for murine ocular neovascularisation, worthy of further mechanistic and pharmacokinetic evaluation.
Systematic Review of the Relation Between Intestinal Microbiota and Toll-Like Receptors in the Metabolic Syndrome: What Do We Know So Far? (2015)

Current evidence suggests that innate immunity and intestinal microbiota may be the hidden link in the metabolic syndrome development mechanisms. In the near future, this can be the key in the development of new prophylactic and therapeutic strategies to treat metabolic syndrome patients.
Enabling Mobile Devices for Home Automation Using ZigBee (2013)

Home automation systems are collections of interconnected devices for controlling various functions within a house, such as light control, heating, air conditioning, etc. Mobile devices are ideal in providing a user interface in a home automation system, due to their portability and their wide range of capabilities. They can communicate with a home automation network through an Internet gateway, but cannot directly communicate with devices in the network, as these devices usually implement low power communication protocols, such as ZigBee. We investigate several methods to equip an Android device with a dongle capable of ZigBee communication. We propose a scalable architecture, with three abstraction layers, that scales over multiple communication channels, such as the TCP channel, for communication with the gateway, and the USB channel, for direct communication with devices through the dongle. We test the application with two client devices running Android 4.0 and a mock home automation network consisting of a couple of ZigBee devices and a PC as gateway. We estimate the energy consumption of WiFi transfers over several typical use cases and we conclude that using ZigBee can prove beneficial in some cases, both in terms of functionality and performance.
Preparation of Electrospun Nanocomposite Nanofibers of Polyaniline/Poly(methyl methacrylate) with Amino-Functionalized Graphene (2017)

In this paper we report upon the preparation and characterization of electrospun nanofibers of doped polyaniline (PANI)/poly(methyl methacrylate) (PMMA)/amino-functionalized graphene (Am-rGO) by electrospinning technique. The successful functionalization of rGO with amino groups is examined by Fourier transforms infrared (FTIR), X-ray photoelectron spectroscopy (XPS) and Raman microspectrometer. The strong electric field enables the liquid jet to be ejected faster and also contributes to the improved thermal and morphological homogeneity of PANI/PMMA/Am-rGO. This results in a decrease in the average diameter of the produced fibers and shows that these fibers can find promising uses in many applications such as sensors, flexible electronics, etc.
Data fusion framework for sand detection in pipelines (2009)

Reliable sand detection is an important component of oil production system. In practice, produced sand in oil pipelines poses a serious problem in many production situations, since a small amount of sand in the produced fluid can result in significant erosion in a very short time stage. A new data fusion framework for sand detection in pipeline is presented. The framework is collecting data from oil pipeline using acoustic sensors (SENACO AS100) and flow analyzer (MC-II) in real time. The framework combines two modules: a wireless receiving and transmission (ReT) module and a data fusion module (DaF). The ReT module implementation is based on TinyOS and Crossbow MICAz motes. In order to optimize between the complexity and accuracy needs, DaF module is implemented using two methods; fuzzy art (FA) and maximum likelihood estimator (MLE). The results show the efficient number of sensors needed and compare between FA and MLE redundant.
Cytotoxic effect of cis-parinaric acid in cultured malignant cells. (1991)

Parinaric acid, a naturally occurring 18-carbon fatty acid containing 4 conjugated double bonds, is toxic to human monocytic leukemia cells at concentrations of 5 microM or less. Conditioning of the medium reduces the cytotoxic effect, suggesting that parinaric acid and not a metabolite is the active agent. The mechanism of parinaric acid toxicity appears to involve lipid peroxidation because the toxic action can be blocked by the addition of butylated hydroxytoluene. When U-937 cells are differentiated to the monocytic form, they become resistant to as much as 30 microM parinaric acid. This difference in sensitivity may be explained in part by the fact that the undifferentiated cells take up 3 to 4 times more parinaric acid. Concentrations of parinaric acid less than 5 microM are also toxic to human THP-1 monocytic leukemia, HL-60 human promyelocytic leukemia, and Y-79 human retinoblastoma cells. Measurements of protein synthesis indicate that differentiated U-937 cells, confluent cultures of human fibroblasts, bovine aortic endothelial cells, and CaCo-2 colonic mucosal cells are much less sensitive to parinaric acid than the malignant cell lines tested, suggesting that the cytotoxic action may be selective for rapidly growing malignant tumors. Thus, parinaric acid may be the prototype of a new class of lipid chemotherapeutic agents that contain a conjugated system of double bonds and act by sensitizing tumor cells to peroxidation.
Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II. (1994)

Mammalian transcription factor IIF (TFIIF) is a heterodimer composed of approximately 30-kDa (RAP30) and approximately 70-kDa (RAP74) subunits. TFIIF has been shown to bind RNA polymerase II and control the activity of the enzyme in both the initiation and elongation stages of transcription. Although previous studies have established a role for RAP30 in assembly of the preinitiation complex and in transcription initiation, information on the function of RAP74 in these processes has been lacking. Using a highly purified transcription system and assays that permit sensitive measurement of the contributions of both RAP30 and RAP74 to TFIIF function, we have investigated the roles of these TFIIF subunits in transcription initiation and elongation. Results of template competition experiments indicate that both RAP30 and RAP74 contribute to the formation of stable preinitiation intermediates containing RNA polymerase II. Investigation of the role of TFIIF in transcription initiation indicates that both RAP30 and RAP74 function in synthesis of the first few phosphodiester bonds of nascent transcripts and in formation of Sarkosyl-resistant pre-initiation intermediates. Finally, kinetic experiments indicate that both RAP30 and RAP74 function in TFIIF-mediated stimulation of the rate of RNA chain elongation by RNA polymerase II.
Compound heterozygous D90A and D96N SOD1 mutations in a recessive amyotrophic lateral sclerosis family (2001)

We describe a French amyotrophic lateral sclerosis (ALS) family with two distinct mutations in the Cu/Zn superoxide dismutase (SOD1) gene. The D90A mutation has been well described and clearly shown to cause recessive ALS. In this family, affected individuals are heterozygous for the D90A mutation and also carry a single copy of a novel SOD1 mutation, D96N. We propose that in this family both mutations are required for the development of disease.
All-reflection Michelson interferometer: analysis and test for far ir Fourier spectroscopy (1978)

The beam splitter of the all-reflection Michelson interferometer consists of a combination of three parallel diffraction gratings. This paper extends the analysis of the instrument to include the effects of lateral errors in the grating adjustment (i.e., displacements parallel to the grating faces and perpendicular to the grooves). Such errors are shown to introduce a phase shift independent of wavenumber and proportional to grating order number. Tests of an instrument designed for Fourier transform spectroscopy in the 500-1000-microm spectral range are reported and shown to be in agreement with the analysis. For wavenumbers which pass through the instrument in 2 or more orders, cross-order interference effects are expected to occur which cause rapid variations in the efficiency vs wavenumber curve. This possibility should be eliminated in the design of a practical instrument. The resolution of the test instrument (1.6 cm(-1)) was insufficient to reveal this effect.
Kinetics of H<sub>2</sub>S absorption into aqueous ferric solutions of edta and hedta (1994)

Abstract Kinetics of the reaction of hydrogen sulfide with ferric chelates of ethylenediaminetetraacetic acid (EDTA) at 22°C and with ferric chelates of hydroxyethyle‐thylenediaminetriacetic acid (HEDTA) from 21°C to 60°C were studied in a stirred cell reactor under industrial conditions. Concentration of the ferric chelates ranged from 40 to 150 mol/m 3 and the pH was varied from 4 to 10. Only the hydroxy forms of the ferric chelates appear to react with hydrogen sulfide. Under the conditions applied, the reaction appeared to be first‐order in hydrogen sulfide and first‐order in hydroxy ferric chelate. At 22°C the rate constant for the EDTA complex is 14–21 m 3 /mol·s. Uncertainty in this constant is due to the characteristics of the EDTA complex at pH values above 7. For the HEDTA complex, the activation energy of the reaction of hydrogen sulfide with the monohydroxy ferric chelate is (26.4 ± 0.6) kJ/mol with k = 1.8 m 3 /mol·s at 25°C. The rate constant for the reaction with the dihydroxy ferric chelate is (300 ± 150) m 3 /mol·s in the temperature range investigated.
Effect of Fat Source on Duodenal Flow of Trans-C18:1 Fatty Acids and Milk Fat Production in Dairy Cows (1997)

The objective of the study was to determine the effects of dietary fat source on duodenal flow, apparent absorption, and milk fat incorporation of trans-C18:1 fatty acids. Four ruminally and duodenally cannulated multiparous Holsteins cows in mid to late lactation were fed a basal diet containing 36% corn silage, 24% alfalfa haylage, and 40% concentrate (dry matter basis). Diets contained 0% supplemental fat (control diet), 3.7% high oleic sunflower oil, 3.7% high linoleic sunflower oil, or 3.7% partially hydrogenated vegetable shortening; treatments were administered in a 4 x 4 Latin square design with 3-wk experimental periods. The flow of trans-C18:1 to the duodenum was higher for cows fed diets supplemented with fat than for cows fed the control diet (283 vs. 64 g/d). Incomplete biohydrogenation accounted for the increased flow of trans-C18:1 to the duodenum in cows fed diets containing high oleic and high linoleic sunflower oil. Increased flow of trans-C18:1 in cows fed the diet containing partially hydrogenated vegetable shortening most likely originated from the trans-C18:1 in the diet. Milk fat percentages were 3.48, 3.07, 3.18, and 3.38% for cows fed the control diet and diets containing high oleic sunflower oil, high linoleic sunflower oil, and vegetable shortening, respectively. Milk trans-C18:1 increased from 2.9 to 11.2% of the total fatty acids for cows fed the control diet and the diets supplemented with fat, respectively. Milk trans-C18:1 were equal across all diets supplemented with fat. Ruminal and total tract digestion of organic matter, neutral detergent fiber, and N did not change with fat supplementation. Results showed that trans-C18:1 is increased in the milk of cows with reduced milk fat; however, excessive amounts of trans-C18:1 in milk do not necessarily correspond directly to milk fat depression.
Association of ADAM33 gene polymorphisms with COPD in a northeastern Chinese population (2009)

These preliminary results suggest an association between ADAM33 polymorphisms and COPD in a Chinese Han population.
Thrombin plays a key role in late platelet thrombus growth and/or stability. Effect of a specific thrombin inhibitor on thrombogenesis induced by aortic subendothelium exposed to flowing rabbit blood. (1994)

Thrombin is involved in the pathogenesis of venous and arterial thrombosis. This study addressed the question of the relative importance of thrombin in the early and late phases of thrombogenesis. The effect of Ro 46-6240 (1.43 mg/kg bolus and 0.1 mg/kg per minute i.v.), a novel, selective thrombin inhibitor on thrombogenesis induced by rabbit aorta subendothelium, was measured ex vivo in a perfusion chamber model after a short (5-minute) and long (30-minute) exposure time to rabbit native blood. The role of the perfusion time was assessed at shear rates of 100/s, 650/s, and 2600/s. These shear rates mimic blood flow conditions found in veins, arteries, and small or stenosed arteries, respectively. Fibrin deposition and platelet thrombus formation on subendothelium were evaluated by microscopic morphometry. In the presence of Ro 46-6240, fibrin deposition was abolished at both perfusion times and at all shear rates. In the 5-minute experiments, thrombus height was reduced by Ro 46-6240 at shear rates of 100/s (85%) and 650/s (35%) but not at a shear rate of 2600/s, whereas thrombus area was not affected at any shear rate. In contrast, both thrombus height and thrombus area were reduced (60% to 90%) by Ro 46-6240 in the 30-minute perfusion groups at all wall shear rates. The antithrombotic effect of Ro 46-6240 after 30-minute perfusion was confirmed by the minimal increase in the pressure difference between the entrance and the exit of the perfusion chamber compared with the control groups.(ABSTRACT TRUNCATED AT 250 WORDS)
Enhanced Production and Secretion of Heterologous Proteins by the Filamentous Fungus <i>Aspergillus oryzae</i> via Disruption of Vacuolar Protein Sorting Receptor Gene <i>Aovps10</i> (2010)

Filamentous fungi have received attention as hosts for heterologous protein production because of their high secretion capability and eukaryotic posttranslational modifications. However, despite these positive attributes, a bottleneck in posttranscriptional processing limits protein yields. The vacuolar protein sorting gene VPS10 encodes a sorting receptor for the recognition and delivery of several yeast vacuolar proteins. Although it can also target recombinant and aberrant proteins for vacuolar degradation, there is limited knowledge of the effect of its disruption on heterologous protein production. In this study, cDNA encoding AoVps10 from the filamentous fungus Aspergillus oryzae was cloned and sequenced. Microscopic observation of the transformant expressing AoVps10 fused with enhanced green fluorescent protein showed that the fusion protein localized at the Golgi and prevacuolar compartments. Moreover, disruption of the Aovps10 gene resulted in missorting and secretion of vacuolar carboxypeptidase AoCpyA into the medium, indicating that AoVps10 is required for sorting of vacuolar proteins to vacuoles. To investigate the extracellular production levels of heterologous proteins, DeltaAovps10 mutants expressing either bovine chymosin (CHY) or human lysozyme (HLY) were constructed. Interestingly, the DeltaAovps10 mutation increased the maximum extracellular production levels of CHY and HLY by 3- and 2.2-fold, respectively. Western blot analysis of extracellular heterologous proteins also demonstrated an improvement in productivity. These results suggest that AoVps10 plays a role in the regulation of heterologous protein secretion in A. oryzae and may be involved in the vacuolar protein degradation through the Golgi apparatus.
Clinical features and pathophysiology of polycystic kidney disease in man. (1976)

Adult polycystic kidney disease is recognized as the traditional form of cystic kidney disease in man. It was the first described. It is the best studied. It is the most common among adults, affecting roughly one of every 500 persons. It accounts for renal failure in some 5% of patients coming to renal transplantation and ranks third as a cause of end-stage renal disease. Its cause(s) and prevention (other than genetic control through counseling) remain unknown, although some evidence of a preliminary nature is beginning to accumulate.
Levels of diphtheria and tetanus specific IgG of Portuguese adult women, before and after vaccination with adult type Td. Duration of immunity following vaccination (2007)

Our study suggests that, to protect against tetanus, there is no need to administer decennial boosters to the Portuguese adults who have complied with the childhood/adolescent schedule (6 doses of tetanus toxoid). The adult booster intervals could be wider, probably of 20 years. This also seems to apply to protection against diphtheria, but issues on the herd immunity and on the circulation of toxigenic strains need to be better understood.
Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein. (1981)

The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC 1.1.1.1) was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid sequence of the ADH. Two intervening DNA sequences (introns) were identified within the protein encoding region: one was 65 nucleotides and located between the codons for amino acid residues 32 and 33, and one was 70 nucleotides and located between the codons for amino acid residues 167 and 168. Both contained the 5' G-T and 3' A-G dinucleotides characteristic of intron boundaries of eukaryotic genes. On the basis of secondary structure predictions, the first 140 amino acid residues of Drosophila ADH are in an alternating beta-sheet/alpha-helix arrangement which is characteristic of the coenzyme binding domain of dehydrogenases. The smaller of the two introns interrupts the domain predicted to bind the adenine portion of the coenzyme.
DEVELOPMENT OF A TESTBED FOR EVALUATION OF ELECTRIC VEHICLE DRIVE PERFORMANCE (1997)

This thesis develops and implements a testbed for the evaluation of inverter fed motor drives used in electric vehicles. The testbed consists of a computer-controlled dynamometer connected to power analysis and data collection tools. The programming and operation and of the testbed is covered. Then it is used to evaluate three pairs of identical rating inverters. The goal is to analyze the effect of topology and software improvements on motor drive efficiency. The first test analyzes the effect of a soft-switching circuit on inverter and motor efficiency. The second test analyzes the difference between space vector modulation (SVM) and current-band hysteresis. The final test evaluates the effect of both soft-switching and SVM on drive performance. The tests begin with a steady state analysis of efficiency over a wide range of torque and speed. Then drive cycles tests are used to simulate both city and highway driving. Together, these dynamic and steady state test results provide a realistic assessment of electric vehicle drive performance.
Draft Genome Sequence of Thermotoga maritima A7A Reconstructed from Metagenomic Sequencing Analysis of a Hydrocarbon Reservoir in the Bass Strait, Australia (2013)

The draft genome sequence of Thermotoga maritima A7A was obtained from a metagenomic assembly obtained from a high-temperature hydrocarbon reservoir in the Gippsland Basin, Australia. The organism is predicted to be a motile anaerobe with an array of catabolic enzymes for the degradation of numerous carbohydrates.