gebhart/patrepeval huggingface dataset:
Effect of Type IIB von Willebrand Disease Mutation Arg(545)Cys on Platelet Glycoprotein Ib Binding – Studies with Recombinant von Willebrand Factor (1993)

Type IIB von Willebrand disease (vWD) is characterized by a selective loss of high molecular weight von Willebrand factor (vWF) multimers in plasma due to their abnormally enhanced reactivity with platelets. Several missense mutations in the platelet glycoprotein Ib (GPIb) binding domain of vWF were recently characterized that cause type IIB vWD. The effect of type IIB mutation Arg(545)Cys on vWF binding to platelet GPIb was studied using recombinant wild type (rvWFWT) and mutant rvWFR545C expressed in COS-7 cells. In the absence of ristocetin, 50% of rvWFR545C bound spontaneously to platelet GPIb and the binding increased to 70% in the presence of 0.2 mg/ml ristocetin; rvWFWT did not bind significantly under either condition. Botrocetin-induced binding of rvWFR545C was only slightly increased compared to rvWFWT. These data demonstrate that the Arg(545)Cys mutation increases the affinity of vWF for GPIb, resulting in the characteristics gain-of-function type IIB vWD phenotype.
Infrared absorption band and vibronic structure of the nitrogen-vacancy center in diamond (2013)

Negatively charged nitrogen-vacancy (NV${}^{\ensuremath{-}}$) color centers in diamond have generated much interest for use in quantum technology. Despite the progress made in developing their applications, many questions about the basic properties of NV${}^{\ensuremath{-}}$ centers remain unresolved. Understanding these properties can validate theoretical models of NV${}^{\ensuremath{-}}$, improve their use in applications, and support their development into competitive quantum devices. In particular, knowledge of the phonon modes of the ${}^{1}{A}_{1}$ electronic state is key for understanding the optical pumping process. Using pump-probe spectroscopy, we measured the phonon sideband of the ${}^{1}E\ensuremath{\rightarrow}{}^{1}{A}_{1}$ electronic transition in the NV${}^{\ensuremath{-}}$ center. From this we calculated the ${}^{1}E\ensuremath{\rightarrow}{}^{1}{A}_{1}$ one-phonon absorption spectrum and found it to differ from that of the ${}^{3}E\ensuremath{\rightarrow}{}^{3}{A}_{2}$ transition, a result which is not anticipated by previous group-theoretical models of the NV${}^{\ensuremath{-}}$ electronic states. We identified a high-energy 169-meV localized phonon mode of the ${}^{1}{A}_{1}$ level.
Effect of polyethylene glycol on the liquid–liquid phase transition in aqueous protein solutions (2002)

We have studied the effect of polyethylene glycol (PEG) on the liquid-liquid phase separation (LLPS) of aqueous solutions of bovine gammaD-crystallin (gammaD), a protein in the eye lens. We observe that the phase separation temperature increases with both PEG concentration and PEG molecular weight. PEG partitioning, which is the difference between the PEG concentration in the two coexisting phases, has been measured experimentally and observed to increase with PEG molecular weight. The measurements of both LLPS temperature and PEG partitioning in the ternary gammaD-PEG-water systems are used to successfully predict the location of the liquid-liquid phase boundary of the binary gammaD-water system. We show that our LLPS measurements can be also used to estimate the protein solubility as a function of the concentration of crystallizing agents. Moreover, the slope of the tie-lines and the dependence of LLPS temperature on polymer concentration provide a powerful and sensitive check of the validity of excluded volume models. Finally, we show that the increase of the LLPS temperature with PEG concentration is due to attractive protein-protein interactions.
Membrane-disrupting iridium(<scp>iii</scp>) oligocationic organometallopeptides (2016)

A series of oligoarginine peptide derivatives containing cyclometallated iridium(iii) units display remarkable cytotoxicity, comparable to that of cisplatin. In vitro studies with unilamellar vesicles support a membrane-disrupting mechanism of action.
System Level Performance of Cellular WiMAX IEEE 802.16 with SDMA-enhanced Medium Access (2007)

Recent years have witnessed an ever-increasing interest in smart antenna technologies to boost the capacity of existing and future wireless systems. As one of the first standards the wireless metropolitan area network IEEE 802.16 (WiMAX) provides means to support these techniques. This paper investigates the potential of smart antenna beamforming and space division multiple access (SDMA) in the context of a cellular IEEE 802.16 deployment. It presents the system level performance evaluation of a joint TDMA/SDMA scheduling approach taking into account the influence of intra- and inter-cell interference generated by concurrent SDMA transmissions. The performance of single- and multi-user beamforming is compared with the non-beamforming reference case.
An Optical Neural Network Architecture based on Highly Parallelized WDM-Multiplier-Accumulator (2019)

Future applications such as anomaly detection in a network and autonomous driving require extremely low, submicrosecond latency processing in pattern classification. Towards the realization of such an ultra-fast inference processing, this paper proposes an optical neural network architecture which can classify anomaly patterns at sub-nanosecond latency. The architecture fully exploits optical parallelism of lights using wavelength division multiplexing (WDM) in vector-matrix multiplication. It also exploits a linear optics with passive nanophotonic devices such as microring resonators, optical combiners, and passive couplers, which make it possible to construct low power and ultra-low latency optical neural networks. Optoelectronic circuit simulation using optical circuit implementation of multi-layer perceptron (MLP) demonstrates sub-nanosecond processing of optical neural network.
Dynamic Mathematical Model for the Biodegradation of VOCs in a Biofilter:  Biomass Accumulation Study (1998)

Although biofiltration is a firmly established technology for the control of emissions of volatile organic compound (VOCs), more fundamental research is still needed. This work uses a mathematical model describing the dynamic physical and biological processes occurring in a packed trickle-bed air biofilter to analyze the relationship between biofilter performance, biomass accumulation in the reactor, and mathematical description of the packed bed porous media. In this study a biofilter packed with pelletized support media was used to treat toluene achieving removal efficiencies over 99% and 97% for 4.1 and 6.2 kg COD/m3 day toluene loading, respectively. Experimental results showed that as biomass accumulates in the reactor, the available area for the contaminant to diffuse into the biofilm decreases causing a drop in removal efficiency. This effect is specially important for biofilters where there is a high degree of biomass accumulation that significantly affects biofilter performance. In response to these observations, a new approach for the calculation of the biofilm specific surface area of the reactor as a function of biomass growth was developed. Three models of the reactor porous medium were analyzed. The medium was represented as a bed of equivalent spheres in the first model, as an equivalent set of parallel pipes in the second model, and as an equivalent set of flat parallel plates in the third model. The first two models, spheres and pipes, were proven superior in their ability to explain the system performance. The effect of contaminant solubility on biofilter performance was also analyzed.
Preparation of silicon carbide‐silicon nitride fibers by the pyrolysis of polycarbosilazane precursors: A review (1986)

Abstract The development of silicon carbide‐silicon nitride (SiC‐Si 3 N 4 ) fibers by the pyrolysis of polycarbosilazane precursors that was carried out in this laboratory is reviewed. Precursor resin, which was prepared by heating tris(N‐methylamino)methylsilane or tris(N‐methylamino)phenyl‐silane to about 520°C, was drawn into fibers from the melt and then made unmeltable by humidity conditioning at 100°C and 95 percent relative humidity. The humidity‐treated precursor fibers were pyrolyzed to ceramic fibers with good mechanical properties and electrical resistivity. For example, SiCSi 3 N 4 fibers derived from tris(N‐methyl‐amino)‐methylsilane had a tensile rupture modulus of 29 × 10 6 psi and electrical resistivity of 6.9 × 10 8 Ω‐cm, which is 10 12 times greater than a value obtained for graphite fibers.
Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger (2011)

Filamentous fungi are the cause of serious human and plant diseases but are also exploited in biotechnology as production platforms. Comparative genomics has documented their genetic diversity, and functional genomics and systems biology approaches are under way to understand the functions and interaction of fungal genes and proteins. In these approaches, gene functions are usually inferred from deletion or overexpression mutants. However, studies at these extreme points give only limited information. Moreover, many overexpression studies use metabolism-dependent promoters, often causing pleiotropic effects and thus limitations in their significance. We therefore established and systematically evaluated a tunable expression system for Aspergillus niger that is independent of carbon and nitrogen metabolism and silent under noninduced conditions. The system consists of two expression modules jointly targeted to a defined genomic locus. One module ensures constitutive expression of the tetracycline-dependent transactivator rtTA2(S)-M2, and one module harbors the rtTA2(S)-M2-dependent promoter that controls expression of the gene of interest (the Tet-on system). We show here that the system is tight, responds within minutes after inducer addition, and allows fine-tuning based on the inducer concentration or gene copy number up to expression levels higher than the expression levels of the gpdA promoter. We also validate the Tet-on system for the generation of conditional overexpression mutants and demonstrate its power when combined with a gene deletion approach. Finally, we show that the system is especially suitable when the functions of essential genes must be examined.
Different Roles for Cyclins D1 and E in Regulation of the G<sub>1</sub>-to-S Transition (1995)

Ectopic expression of cyclins D1 and E was previously shown to accelerate the G1/S-phase transition, indicating that both classes of G1 cyclin control an event(s) that is rate limiting for entry into S phase. In order to determine whether cyclins D1 and E control the same or two different rate-limiting events, we have created cell lines that express both cyclins in an inducible manner. We show here that ectopic expression of both cyclins E and D1 in the same cell has an additive effect on shortening of the G1 interval relative to expression of any single cyclin. In order to further explore the molecular basis for G1 cyclin action, we used cell lines capable of expressing cyclin D1, E, or both prematurely and measured the effect of cyclin expression in early G1 on phosphorylation of the retinoblastoma susceptibility gene product (pRb). We show here that while premature expression of either cyclin alone advances the G1/S-phase transition to the same extent, premature expression of cyclin D1 leads to immediate appearance of hyperphosphorylated pRb, while premature expression of cyclin E does not. Ectopic expression of both cyclins E and D1 in the same cell has an additive effect on shortening of the G1 interval, while the effect on pRb phosphorylation is similar to the effect of cyclin D1 alone. These results suggest that cyclins E and D1 control two different events, both rate limiting for the G1/S-phase transition, and that pRb phosphorylation might be the rate-limiting event controlled by cyclin D1.
Prognostic implications of BCL6 rearrangement in uniformly treated patients with diffuse large B-cell lymphoma - a Nordic Lymphoma Group study (2002)

The purpose of this study was to investigate the prognostic implications of BCL6 rearrangement in a uniformly treated population of patients with diffuse large B-cell lymphoma (DLBCL) and to characterise the relationship between BCL6 rearrangement and prognostic factors. A total of 269 patients with DLBCL entered a randomised trial comparing the chemotherapy regimen CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) to the MACOP-B (methotrexate, doxorubicin, cyclophosphamide, vincristine, prednisone, bleomycin) regimen. In 44 cases, frozen tissue was available for assessment of BCL6 status by Southern blot analysis. BCL6 was rearranged in six of 43 evaluable cases (14%), and was associated with elevated lactate dehydrogenase (LDH), and a higher patient age. No association between BCL6 status and expression of BCL2, Ki-67 or TP53 was found. Patients presenting with BCL6 rearrangement displayed a weak trend towards better overall and failure-free survival (67 and 67% at 5 years), compared to patients with germline BCL6 (63 and 52%), but the difference was not statistically significant. In accordance with previously published series, the presence of BCL6 rearrangement does not define a prognostically distinct subgroup of DLBCL. Assessment of BCL6 status may, however, be of clinical interest when related to other prognostic variables.
Biomechanical regulation of mesenchymal cell function (2012)

Progress in understanding biomechanical regulation of mesenchymal cell function is leading to novel approaches for improving clinical outcomes in fibrotic diseases and wound healing. These approaches include interventions aimed at modifying the tissue biomechanical environment, and efforts to target mesenchymal cell activation by, and reciprocal interactions with, the mechanical environment.
A Bacterial Guanine Nucleotide Exchange Factor Activates ARF on <i>Legionella</i> Phagosomes (2002)

The intracellular pathogen Legionella pneumophila subverts vesicle traffic in eukaryotic host cells to create a vacuole that supports replication. The dot/icm genes encode a protein secretion apparatus that L. pneumophila require for biogenesis of this vacuole. Here we show that L. pneumophila produce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases). The RalF protein is required for the localization of ARF on phagosomes containing L. pneumophila. Translocation of RalF protein through the phagosomal membrane is a dot/icm-dependent process. Thus, RalF is a substrate of the Dot/Icm secretion apparatus.
An integrated design and characterization environment for the development of a standard cell library (2002)

A design environment is described for the development of a standard cell library. This environment controls a central database called the Cell Data File (CDF). The CDF is a flexible database format for describing a cell technology library. CDF contains all the information necessary to build icon, schematic, and layout views from module generators or custom-crafted cells. Characterization data (transport delays, delay/pf, setup and hold times) can be automatically measured and inserted back in the CDF for translation to logic synthesis timing tables and functional models.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Porous Glass‐Ceramic in the CαO–TiO <sub>2</sub> –P <sub>2</sub> O <sub>5</sub> System (1989)

A porous glass‐ceramic in the CaO–TiO 2 —P 2 O 5 system has been prepared by crystallization and subsequent chemical leaching of the corresponding glass. By applying a two‐step heat treatment to 45CaO · 25TiO 2 · 30P 2 O 5 glasses containing a few mol% of Na 2 O, volume crystallization results in the formation of dense glass‐ceramics composed of CaTi 4 (PO 4 ) 6 and β‐Ca 3 (PO 4 ) 2 phases. By leaching the resultant glass ceramics with HCI, β‐Ca 3 (PO 4 ) 2 is selectively dissolved out, leaving a crystalline CaTi 4 (PO 4 ) 6 skeleton. The surface area and mean pore radius of the porous glass‐ceramics were approximately 40 m 2 /g and 13 nm, respectively.
Synthesis of four methoxy-substituted 1,8-naphthalic anhydrides and of the three monomethyl-1,8-naphthalic anhydrides (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of four methoxy-substituted 1,8-naphthalic anhydrides and of the three monomethyl-1,8-naphthalic anhydridesJames Cason, Andreas Weiss, and Stephen A. MontiCite this: J. Org. Chem. 1968, 33, 9, 3404–3408Publication Date (Print):September 1, 1968Publication History Published online1 May 2002Published inissue 1 September 1968https://doi.org/10.1021/jo01273a010Request reuse permissionsArticle Views490Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (797 KB) Get e-Alertsclose Get e-Alerts
Spektroskopische Untersuchungen an Orange I und Orange II (1952)

Abstract Das Lösungsspektrum der α‐Naphtochinon‐methylphenylhydra‐zon‐p‐sulfosäure (N‐Methyl‐Orange I) wurde erstmals aufgenommen. Durch Vergleich konnte festgestellt werden, dass Orange I in neutraler, wässeriger Lösung („saure”︁ Form) in der Chinonhydrazon‐Form vorliegt. Ebenso besteht die „saure”︁ Form von Orange II ganz überwiegend aus der Chinonhydrazon‐Form.
A centrifugo-pneumatic cascade for fully integrated and multiplexed biological analysis (2012)

This work introduces a toolkit of structures based on centrifugo-pneumatic actuation that allows for liquid loading/transfer, metering, mixing and sedimentation in a microstructured polymer disc. This pneumatic cascade leverages full integration and automation of a range of bioassays initiated by a single sample reagent loading phase and using the same fluidic pathway for a sequence of sample and reagents. The centrifugo-pneumatic cascade also reduces manufacturing complexity as there is no need for valving based on surface coatings, geometrical microfeatures or sacrificial inlays common in other automation schemes. As a proof of concept of a homogeneous bioassay, we detect nitric oxide in whole blood by colorimetry.
Distributed source localization using ESPRIT algorithm (2001)

A new algorithm based on ESPRIT is proposed for the estimation of the central angle and angular extension of distributed sources. The central angles are estimated using TLS-ESPRIT for both incoherently distributed (ID) and coherently distributed (CD) sources. For CD sources, the extension width is estimated by constructing a one-dimensional (1-D) distributed source parameter estimator (DSPE) spectrum for each source. For ID sources, the extension widths are estimated using the central moments of the distribution. The algorithm can be used for sources with different angular distributions.
Generative Event Schema Induction with Entity Disambiguation (2015)

Conference of 53rd Annual Meeting of the Association for Computational Linguistics and the 7th International Joint Conference on Natural Language Processing of the Asian Federation of Natural Language Processing, ACL-IJCNLP 2015 ; Conference Date: 26 July 2015 Through 31 July 2015; Conference Code:114195
Lentivector Transduction Improves Outcomes Over Transplantation of Human HSCs Alone in NOD/SCID/Fabry Mice (2012)

Fabry disease is a lysosomal storage disorder caused by a deficiency of α-galactosidase A (α-gal A) activity that results in progressive globotriaosylceramide (Gb(3)) deposition. We created a fully congenic nonobese diabetic (NOD)/severe combined immunodeficiency (SCID)/Fabry murine line to facilitate the in vivo assessment of human cell-directed therapies for Fabry disease. This pure line was generated after 11 generations of backcrosses and was found, as expected, to have a reduced immune compartment and background α-gal A activity. Next, we transplanted normal human CD34(+) cells transduced with a control (lentiviral vector-enhanced green fluorescent protein (LV-eGFP)) or a therapeutic bicistronic LV (LV-α-gal A/internal ribosome entry site (IRES)/hCD25). While both experimental groups showed similar engraftment levels, only the therapeutic group displayed a significant increase in plasma α-gal A activity. Gb(3) quantification at 12 weeks revealed metabolic correction in the spleen, lung, and liver for both groups. Importantly, only in the therapeutically-transduced cohort was a significant Gb(3) reduction found in the heart and kidney, key target organs for the amelioration of Fabry disease in humans.
Exercise in pregnancy. (2002)

This article is a brief overview of a vast area of interest, in which we are learning that women can be encouraged to help their bodies cope with the physiological adaptations of pregnancy, and to enjoy what is usually a very special time in their lives. Although the studies carried out have only been very small, moderate exercise has not shown any harmful effects in healthy pregnant women, and these findings encourage us, as healthcare professionals, to support women in their choice to exercise during pregnancy, with known beneficial outcomes for both mother and baby.
Design and Implementation of a Rate Control Mechanism for Image-based Virtual Exploration over Wireless Networks (2006)

In recent years, we have witnessed a growing interest in multimedia streaming applications over wireless networks. However, while a number of wireless multimedia applications have already been proposed, many issues related to bandwidth management of 3D multimedia streaming over wireless networks remains one of the most challenging task one has to deal with. In this paper, we propose an adaptive rate control algorithm that uses an immediate feedback mechanism in order to determine the network status. In our scheme, both packet loss and round-trip time are used as indicators of whether or not a network is congested, so as to differentiate end-to-end traffic congestion from wireless link errors. Furthermore, in order to pre-fetch images to the client, we propose to use a motion prediction technique. We discuss our schemes, describe their implementation, and present an extensive set of simulation experiments in order to evaluate their performance using ns-2 simulator.
Epidemiology of Central Line-Associated Bloodstream Infections in the Pediatric Intensive Care Unit (2011)

The 2008 CLA-BSI definition change decreased the measured incidence. The daily CLA-BSI risk was very low in patients during the first 7 days of catheterization but doubled thereafter. The risk of CLA-BSI was lower in patients with lines inserted in the jugular vein and higher in patients with gastrointestinal and oncologic diagnoses. These patients are target populations for additional study and intervention.