gebhart/patrepeval huggingface dataset:
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells (2010)

Human embryonic stem cells (hESC) represent a new source of stem cells that can be propagated and expanded in vitro indefinitely, providing a potentially inexhaustible and donorless source of cells for human therapy. The ability to create banks of hESC lines with matched or reduced incompatibility could potentially reduce or eliminate the need for immunosuppressive drugs and/or immunomodulatory protocols altogether, for example, O-type RhD(-) lines for generation of universal red blood cells (RBC). Hematopoietic differentiation of hESCs has been extensively investigated in vitro, and hematopoietic precursors as well as differentiated progeny representing erythroid, myeloid, macrophage, megakaryocytic, and lymphoid lineages have been identified in differentiating hESC cultures. Previous studies also generated primitive erythroid cells from hESCs by embryoid body (EB) formation and coculturing with stromal cells. However, the efficient and controlled differentiation of hESCs into homogeneous RBC populations with oxygen-carrying capacity has not been previously achieved. In this chapter, we describe a robust system that can efficiently generate large numbers of hemangioblasts from multiple hESC lines using well-defined conditions and produce functional homogeneous RBCs with oxygen-carrying capacity in large scale. The homogeneous erythroid cells can be used for further mechanism studies.
Oxidative Cross‐Linking of Pentosans by a Fungal Laccase and Horseradish Peroxidase: Mechanism of Linkage Between Feruloylated Arabinoxylans (1998)

ABSTRACT The potential of a laccase from the fungus Pycnoporus cinnabarinus to cross‐link feruloylated soluble wheat arabinoxylans was investigated using capillary viscometry, size‐exclusion HPLC, and reverse‐phase HPLC of phenolic compounds. The laccase results were compared with those for a hydrogen peroxide/horseradish peroxidase system. The oxidants provoked an increase in viscosity of a 0.2% (w/v) arabinoxylan solution. A gel was formed after 30 min with laccase. Hydrogen peroxide was consumed rapidly before a gel could be formed. Free ferulic acid, methyl ferulate, and vanillic acid inhibited the gelation, whereas fumaric acid had no effect. This suggests that the aromatic ring, and not the propenoic chain of ferulic acid, was the initiating site for arabinoxylan cross‐linking. Ferulic acid and its 8‐ O ‐4′, 8‐5′, and 5‐5′ dehydrodimers were present in nonoxidized arabinoxylans. Upon oxidation, the 8‐8′ and 8‐5′ benzofuran dehydrodimers appeared and the 8‐ O ‐4′ and 8‐5′ dimers increased. The production of dimers was proportional to the consumption of ester‐bound ferulic acid. In cross‐linked arabinoxylans, the major dimers were 8‐5′ benzofuran, 8‐8′, and 8‐ O ‐4′, whereas the 5‐5′ dehydrodimer remained at the same level as in the nonoxidized solution.
Endothelial cell loss after small incision cataract surgery (1970)

There is 15.83 % reduction in endothelial cell count after SICS with PC IOL implantation, which is comparable with other modes of cataract surgery like extra-capsular cataract extraction and phacoemulsification.
Understanding Java stack inspection (2002)

Current implementations of Java make security decisions by searching the runtime call stack. These systems have attractive security properties, but they have been criticized as being dependent on specific artifacts of the Java implementation. The paper models the stack inspection algorithm in terms of a well understood logic for access control and demonstrates how stack inspection is a useful tool for expressing and managing complex trust relationships. We show that an access control decision based on stack inspection corresponds to the construction of a proof in the logic, and we present an efficient decision procedure for generating these proofs. By examining the decision procedure, we demonstrate that many statements in the logic are equivalent and can thus be expressed in a simpler form. We show that there are a finite number of such statements, allowing us to represent the security state of the system as a pushdown automaton. We also show that this automaton may be embedded in Java by rewriting all Java classes to pass an additional argument when a procedure is invoked. We call this security passing style and describe its benefits over previous stack inspection systems. Finally, we show how the logic allows us to describe a straightforward design for extending stack inspection across remote procedure calls.
Hypersensitivity to mGluR5 and ERK1/2 Leads to Excessive Protein Synthesis in the Hippocampus of a Mouse Model of Fragile X Syndrome (2010)

Fragile X syndrome (FXS) is caused by loss of the FMR1 gene product FMRP (fragile X mental retardation protein), a repressor of mRNA translation. According to the metabotropic glutamate receptor (mGluR) theory of FXS, excessive protein synthesis downstream of mGluR5 activation causes the synaptic pathophysiology that underlies multiple aspects of FXS. Here, we use an in vitro assay of protein synthesis in the hippocampus of male Fmr1 knock-out (KO) mice to explore the molecular mechanisms involved in this core biochemical phenotype under conditions where aberrant synaptic physiology has been observed. We find that elevated basal protein synthesis in Fmr1 KO mice is selectively reduced to wild-type levels by acute inhibition of mGluR5 or ERK1/2, but not by inhibition of mTOR (mammalian target of rapamycin). The mGluR5-ERK1/2 pathway is not constitutively overactive in the Fmr1 KO, however, suggesting that mRNA translation is hypersensitive to basal ERK1/2 activation in the absence of FMRP. We find that hypersensitivity to ERK1/2 pathway activation also contributes to audiogenic seizure susceptibility in the Fmr1 KO. These results suggest that the ERK1/2 pathway, and other neurotransmitter systems that stimulate protein synthesis via ERK1/2, represent additional therapeutic targets for FXS.
Mutant Huntingtin Fragments Form Oligomers in a Polyglutamine Length-dependent Manner in Vitro and in Vivo (2010)

Huntington disease (HD) is caused by an expansion of more than 35-40 polyglutamine (polyQ) repeats in the huntingtin (htt) protein, resulting in accumulation of inclusion bodies containing fibrillar deposits of mutant htt fragments. Intriguingly, polyQ length is directly proportional to the propensity for htt to form fibrils and the severity of HD and is inversely correlated with age of onset. Although the structural basis for htt toxicity is unclear, the formation, abundance, and/or persistence of toxic conformers mediating neuronal dysfunction and degeneration in HD must also depend on polyQ length. Here we used atomic force microscopy to demonstrate mutant htt fragments and synthetic polyQ peptides form oligomers in a polyQ length-dependent manner. By time-lapse atomic force microscopy, oligomers form before fibrils, are transient in nature, and are occasionally direct precursors to fibrils. However, the vast majority of fibrils appear to form by monomer addition coinciding with the disappearance of oligomers. Thus, oligomers must undergo a major structural transition preceding fibril formation. In an immortalized striatal cell line and in brain homogenates from a mouse model of HD, a mutant htt fragment formed oligomers in a polyQ length-dependent manner that were similar in size to those formed in vitro, although these structures accumulated over time in vivo. Finally, using immunoelectron microscopy, we detected oligomeric-like structures in human HD brains. These results demonstrate that oligomer formation by a mutant htt fragment is strongly polyQ length-dependent in vitro and in vivo, consistent with a causative role for these structures, or subsets of these structures, in HD pathogenesis.
Clinical Inertia in Individualising Care for Diabetes: Is There Time to do More in Type 2 Diabetes? (2014)

Clinical inertia is defined as the failure to establish appropriate targets and escalate treatment to achieve treatment goals. It accounts for a significant proportion of failure to achieve targets in the management of diabetes and contributes to up to 200,000 adverse diabetes- related outcomes per year. Despite a growing awareness of the phenomenon, and newer, better-tolerated agents for the control of diabetes, there has been little improvement over the last decade in the prevalence of clinical inertia. Although common-place in clinical practice, clinical inertia does not appear to affect clinical trials. There are lessons that may be translated from these randomised controlled trials to clinical practice, which that may improve the care for those with diabetes. Key amongst these interventions are good education, clear treatment strategy and more time for interaction between physician and patients, all of which appears to reduce clinical inertia as evidenced by the "placebo effect" of clinical trials. We plan to review here, the lessons that can be learnt from clinical trials and how these may translate to better care for people with diabetes.
Across the Board (2008)

At the end of the two-year term of the Australian Publishers Association (APA) Board, APA president Juliet Rogers looks back at the achievements of the past year.
Mixed Dementia (2004)

Currently available medications provide only modest clinical benefits once a patient has developed mixed dementia. Cardiovascular risk factor control, especially for hypertension and hyperlipidemia, as well as other interventions to prevent recurrent stroke, likely represent important strategies for preventing or slowing the progression of mixed dementia. Additional research is needed to define better what individuals and families hope to achieve from dementia treatment and to determine the most appropriate use of medication to achieve these goals.
A spectral/temporal method for robust fundamental frequency tracking (2008)

In this paper, a fundamental frequency (F(0)) tracking algorithm is presented that is extremely robust for both high quality and telephone speech, at signal to noise ratios ranging from clean speech to very noisy speech. The algorithm is named "YAAPT," for "yet another algorithm for pitch tracking." The algorithm is based on a combination of time domain processing, using the normalized cross correlation, and frequency domain processing. Major steps include processing of the original acoustic signal and a nonlinearly processed version of the signal, the use of a new method for computing a modified autocorrelation function that incorporates information from multiple spectral harmonic peaks, peak picking to select multiple F(0) candidates and associated figures of merit, and extensive use of dynamic programming to find the "best" track among the multiple F(0) candidates. The algorithm was evaluated by using three databases and compared to three other published F(0) tracking algorithms by using both high quality and telephone speech for various noise conditions. For clean speech, the error rates obtained are comparable to those obtained with the best results reported for any other algorithm; for noisy telephone speech, the error rates obtained are lower than those obtained with other methods.
Mitomycin C "straight scleral tunnel incision"--trabeculectomy with a releasable suture. (2006)

"Straight scleral tunnel incision" -trabeculectomy with a releasable suture supplemented with MMC can reduce complications and get satisfactory results in reducing IOP and SIA.
Alteration of Lipoprotein Profile during Total Parenteral Nutrition with Intralipid 10% (1986)

Eight patients were studied for lipoprotein profiles over a period of 3-7 weeks. Four patients received total parenteral nutrition (TPN), including 1000 ml/day of Intralipid 10%. Three patients received fat-free TPN, and one patient was tube fed 1000 ml/day of Intralipid 10% enterally. Fat-free TPN lowered plasma lipid, especially low density lipoprotein (LDL) and high density lipoproteins (HDL). On the other hand, intravenous administration of Intralipid 10% caused a marked increase of LDL, together with increases of phospholipid and cholesterol, especially free cholesterol. Triglyceride, VLDL, and HDL remained within the normal range in this group. Enteral administration of the same amount of Intralipid 10% did not cause a rise of LDL. Lipid composition of the increased LDL approximated that of lipoprotein X with the intravenous Intralipid 10%. From these findings, we suggest that phospholipids in Intralipid 10% formed abnormal LDL as the result of mobilization of cholesterol from extravascular tissues, when administered intravenously.
Emission power of an electric dipole in the photonic band structure of the fcc lattice (1995)

It has been suggested that spontaneous emission can be inhibited if atomic transition frequencies fall inside photonic band gaps, that is, three-dimensional frequency stop bands of electromagnetic waves generated by three-dimensional periodic dielectric materials (photonic crystals). There has been a growing interest in how atomic emission spectra are changed quantitatively inside the photonic crystals. We develop a classical theory for the calculation of the emission power from the electric dipole located in three-dimensional photonic crystals by incorporating the plane-wave method, dyadic Green’s function, Poynting theorem, and tetrahedron k-space integration. With the method we perform numerical computations for the emission power of an electric dipole located in the photonic crystals of the fcc lattice structure with spherical atoms. The results show the total inhibition of emission in the photonic band gap as well as strong enhancement around the band edges. In addition, the data indicate the strong dependencies of the emission spectrum on the dipole position and the dipole moment in the photonic crystal.
Epigenetic silencing of TET2 and TET3 induces an EMT-like process in melanoma (2016)

Epithelial-Mesenchymal Transition (EMT) is a critical step in the progression of cancer. Malignant melanoma, a cancer developed from pigmented melanocytes, metastasizes through an EMT-like process. Ten-eleven translocation (TET) enzymes, catalyzing the conversion of 5-methylcytosine (5mC) to 5-hydroxylmethylcytosine (5-hmC), are down regulated in melanoma. However, their roles in the progression and the EMT-like process of melanoma are not fully understood. Here we report that DNA methylation induced silencing of TET2 and TET3 are responsible for the EMT-like process and the metastasis of melanoma. TET2 and TET3 are down regulated in the TGF-β1-induced EMT-like process, and the knocking down of TET2 or TET3 induced this EMT-like process. A DNA demethylating agent antagonized the TGF-β-induced suppression of TET2 and TET3. Furthermore, a ChIP analysis indicated that enhanced recruitment of DNMT3A (DNA Methyltransferase 3A) is the mechanism by which TGF-β induces the silencing of TET2 and TET3. Finally, the overexpression of the TET2 C-terminal sequence partially rescues the TGF-β1-induced EMT-like process in vitro and inhibits tumor growth and metastasis in vivo. Hence, our data suggest an epigenetic circuitry that mediates the EMT activated by TGF-β. As an effector, DNMT3A senses the TGF-β signal and silences TET2 and TET3 promoters to induce the EMT-like process and metastasis in melanoma.
Resource management of task oriented distributed sensor networks (2002)

In this paper we provide a foundation for a unified analysis of both the decision fusion and congestion avoidance aspects of a task-oriented distributed sensor network (DSN). Such a framework allows network resource management to be carried out in a manner that is sensitive to the overall objectives of the DSN rather than decoupling them via perhaps simple fairness strategies. The proposed approach associates importance measures related to the degradation and relevance of incoming data lines at each network node. These are then used to carry out intelligent resource management and avoid congestion in a manner which is implicitly related to the decision objectives of the DSN and explicitly related to the resource availability. To achieve this, we propose a 'per-flow' technique that decouples data flow among nodes at different hierarchical levels of the DSN. The resulting framework allows seamless integration of the importance measures providing resource management decisions that are sensitive to the overall DSN objectives.
A modular control station for semiautonomous mobile systems (2005)

In both the Research Institute for Human Engineering (FAT) and the Bochum Polytechnic a modular control station is being used for the development of autonomous mobile systems in a cooperative effort. In the FAT this control station is used to operate a robot vehicle with the dimensions of a fork lift truck. This vehicle performs driving missions in a factory hall. At the Bochum Polytechnis the same control station is used to operate a smaller robot vehicle (100 cm high and 50 cm wide) in the campus building. The development of the control station had to take into account completely different vehicles travelling at incompatible speeds in a different environment. Thus the systems approach started with a general design of a control station for an operator to guide a mobile land robot for a wide range of possible missions. The base software is composed of numerous hardware independent modules. The operator uses an approach with two monitors which present for example digital maps, display sensor data but also a normal TV picture from cameras on the robot vehicle. With the modular control station approach it should be easy to develop new applications for completely different environments and vehicle cinematics and to cooperate in the ongoing development of autonomous system components.
Prevalence of serious eye disease and visual impairment in a north London population: population based, cross sectional study (1998)

Untreated visual impairment and eye disorders affect a substantial proportion of people aged 65 years and older. These findings should contribute to the setting up of future strategies for preservation of sight and eye health services in general.
Prospective Trial of Chemotherapy and Donor Leukocyte Infusions for Relapse of Advanced Myeloid Malignancies After Allogeneic Stem-Cell Transplantation (2002)

PURPOSE: Patients with advanced myeloid malignancies who experience relapse after allogeneic bone marrow transplantation (BMT) have a poor prognosis. Long-term survival after chemotherapy alone, second myeloablative transplant, or donor leukocyte infusions (DLIs) alone is unusual. DLIs may have minimal effectiveness in advanced disease because adequate cellular responses are not able to develop in the presence of bulky, fast-growing disease. A chemotherapy strategy was used to debulk disease before administration of granulocyte colony-stimulating factor (G-CSF)–primed DLIs. PATIENTS AND METHODS: Sixty-five patients experiencing hematologic relapse of myeloid malignancy after HLA-matched sibling BMT were prospectively treated with cytarabine-based chemotherapy, then G-CSF–primed DLIs. No prophylactic immunosuppression was provided. RESULTS: Twenty-seven of 57 assessable patients experienced a complete response. Graft-versus-host disease (GVHD) was observed in 56% of the patients. Treatment-related mortality was 23%. Overall survival at 2 years for the entire cohort was 19%. Patients with a complete response were more likely to survive, with 1- and 2-year survival rates of 51% and 41%, respectively, with a median follow-up of more than 2 years. The 1-year survival for nonresponders was 5%. A posttransplant remission lasting more than 6 months before relapse was associated with a higher likelihood of response. GVHD was not required for durable remission. CONCLUSION: Salvage treatment with chemotherapy before DLI can help some patients with advanced myeloid relapse and is not dependent on GVHD. Patients with short remissions after BMT are unlikely to benefit from this approach, and the approach is associated with significant treatment-related mortality. Modifications of this approach or entirely different approaches will be required for most patients with this difficult clinical problem.
Continuous Stress Detection Using Wearable Sensors in Real Life: Algorithmic Programming Contest Case Study (2019)

The negative effects of mental stress on human health has been known for decades. High-level stress must be detected at early stages to prevent these negative effects. After the emergence of wearable devices that could be part of our lives, researchers have started detecting extreme stress of individuals with them during daily routines. Initial experiments were performed in laboratory environments and recently a number of works took a step outside the laboratory environment to the real-life. We developed an automatic stress detection system using physiological signals obtained from unobtrusive smart wearable devices which can be carried during the daily life routines of individuals. This system has modality-specific artifact removal and feature extraction methods for real-life conditions. We further tested our system in a real-life setting with collected physiological data from 21 participants of an algorithmic programming contest for nine days. This event had lectures, contests as well as free time. By using heart activity, skin conductance and accelerometer signals, we successfully discriminated contest stress, relatively higher cognitive load (lecture) and relaxed time activities by using different machine learning methods.
On the Performance of Nyquist-WDM Terabit Superchannels Based on PM-BPSK, PM-QPSK, PM-8QAM or PM-16QAM Subcarriers (2010)

We investigated through simulations the performance of Nyquist-WDM Terabit superchannels implemented using polarization-multiplexed phase shift-keying based on 2 (PM-BPSK) and 4 (PM-QPSK) signal points or polarization-multiplexed quadrature amplitude modulation based on 8 (PM-8QAM) and 16 (PM-16QAM) signal points. Terabit superchannels are obtained through the aggregation of multiple subcarriers using the Nyquist-WDM technique, based on a tight spectral shaping of each subcarrier which allows very narrow spacing. We first studied the optimum transmitter/receiver filtering in a back-to-back configuration. Then we investigated the maximum reach for different spectral efficiencies, after nonlinear propagation over uncompensated links with lumped amplification. Performance for systems based on both standard single-mode fiber (SSMF) and large effective area non-zero dispersion-shifted fiber (NZDSF) has been analyzed. Assuming SSMF with 25-dB span loss, we found that PM-BPSK can reach 6480 km at a net capacity of 4 Tb/s across the C band. Conversely, PM-16QAM can deliver 27 Tb/s, but over 270 km only. Note that a lower span length, the use of Raman amplification and/or pure silica-core fibers (PSCFs) can significantly increase the maximum reach, but without changing the hierarchy among the performance of modulation formats. We also show that the maximum reachable distance is approximately 2/3 of the one achievable in linear propagation at the optimum launch power, regardless of the modulation format, spacing and fiber type. As additional results, we also verified that the optimum launch power per subcarrier linearly depends on the span loss, varies with the fiber type, but it is independent of the modulation format, and that the relationship between the maximum reachable distance and the span loss is almost linear.