gebhart/patrepeval huggingface dataset:
Effect of Nuclear Factor κB Inhibition on Serotype 9 Adeno-Associated Viral (AAV9) Minidystrophin Gene Transfer to the mdx Mouse (2012)

Gene therapy studies for Duchenne muscular dystrophy (DMD) have focused on viral vector-mediated gene transfer to provide therapeutic protein expression or treatment with drugs to limit dystrophic changes in muscle. The pathological activation of the nuclear factor (NF)-κB signaling pathway has emerged as an important cause of dystrophic muscle changes in muscular dystrophy. Furthermore, activation of NF-κB may inhibit gene transfer by promoting inflammation in response to the transgene or vector. Therefore, we hypothesized that inhibition of pathological NF-κB activation in muscle would complement the therapeutic benefits of dystrophin gene transfer in the mdx mouse model of DMD. Systemic gene transfer using serotype 9 adeno-associated viral (AAV9) vectors is promising for treatment of preclinical models of DMD because of vector tropism to cardiac and skeletal muscle. In quadriceps of C57BL/10ScSn-Dmd(mdx)/J (mdx) mice, the addition of octalysine (8K)-NF-κB essential modulator (NEMO)-binding domain (8K-NBD) peptide treatment to AAV9 minidystrophin gene delivery resulted in increased levels of recombinant dystrophin expression suggesting that 8K-NBD treatment promoted an environment in muscle tissue conducive to higher levels of expression. Indices of necrosis and regeneration were diminished with AAV9 gene delivery alone and to a greater degree with the addition of 8K-NBD treatment. In diaphragm muscle, high-level transgene expression was achieved with AAV9 minidystoophin gene delivery alone; therefore, improvements in histological and physiological indices were comparable in the two treatment groups. The data support benefit from 8K-NBD treatment to complement gene transfer therapy for DMD in muscle tissue that receives incomplete levels of transduction by gene transfer, which may be highly significant for clinical applications of muscle gene delivery.
Delayed Thermal Excimer Fluorescence of Acriflavine in a Stretched PVA Sheet (1967)

Abstract A long-lived emission of acriflavine absorbed into a polyvinylalcohol sheet has been investigated. At higher concentrations, a new, previously-unobserved emission band has been observed. It has been shown that the newly-observed band can be interpreted by a mechanism in which the thermal excitation of the triplet state to the excimer state takes place, followed by an emission. The term “delayed thermal excimer fluorescence” has been given to specify the emission of this type.
Attenuated live cholera vaccine strain CVD 103-HgR elicits significantly higher serum vibriocidal antibody titers in persons of blood group O (1995)

Persons of blood group O are at increased risk of developing cholera gravis. In a randomized, placebo-controlled, double-blind safety-immunogenicity trial of live oral cholera vaccine CVD 103-HgR in 5- to 9-year-old Chilean children, vibriocidal antibody seroconversion (74% overall) did not differ by blood group. However, the reciprocal geometric mean titer (GMT) in blood group O vaccines (GMT = 486) was higher than that in non-O vaccines (GMT = 179) (P < 0.02).
Secure multimedia streaming with trusted digital rights management (2005)

Content protection is now becoming more and more important for digital rights management (DRM), which involves rights embedding, identification, rights validation digital multimedia resource is popular in the real world, how to protect multimedia content from be violated without rights control is an important thing especially to resist copy-spread kind violation. In this paper, an novel approach for multimedia rights management is proposed based on partial encryption method, which can control multimedia resource played in a rights-constraint environment, which can protect multimedia resource from being copying and spreading, and in the authorization usage environment, the protected multimedia is properly played as normal, however once the resource is beyond the authorization environment, the protected resource will not be played correctly. Experiments showed our proposed partial encryption approach was efficient with real-time quality of service, which was suitable for online multimedia streaming in content delivery network.
Electron‐impact‐induced fragmentation of some isomeric cyclopropyl picolyl and pyridyl ketones (1975)

Abstract The electron‐impact‐induced fragmentation of three cyclopropyl picolyl ketones and three cyclopropyl pyridyl ketones has been studied with the aid of deuterium labeling, high resolution mass measurements and metastable mode operation.
Transition structures for the allylboration reactions of formaldehyde by allylborane and allylboronic acid (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTransition structures for the allylboration reactions of formaldehyde by allylborane and allylboronic acidYi Li and Kendall N. HoukCite this: J. Am. Chem. Soc. 1989, 111, 4, 1236–1240Publication Date (Print):February 1, 1989Publication History Published online1 May 2002Published inissue 1 February 1989https://pubs.acs.org/doi/10.1021/ja00186a011https://doi.org/10.1021/ja00186a011research-articleACS PublicationsRequest reuse permissionsArticle Views784Altmetric-Citations77LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Speed and Load Stress in a Sensori-Motor Skill (1951)

Experimental psychology has long been dominated by the proposition that a knowledge of sensory thresholds would provide a framework for the study of human behaviour. Some serious shortcomings of the traditional psychophysical methods for ascertaining these thresholds have been pointed out by Bartlett (1947), who stressed the fact that the discrete stimulus-response situation of psycho physical experiments can provide little information about the sensory processes involved in life, where the subject is dealing always with a series of stimuli. At the same time, psychology has been equally guilty of failing to recognize that under natural conditions perception of a single series of stimuli is not much more than a theoretical possibility. In fact, behaviour must be determined by the percep tion not only of concurrent series of stimuli in different sensory modes, but also of concurrent, but not necessarily related, series of stimuli in the same mode. Particularly one cannot assume that any one sensory threshold determines the framework of behaviour without taking into account the operation of the other thresholds which are almost certain to be present.
Pressure–Volume Curves and Compliance in Acute Lung Injury: Evidence of Recruitment Above the Lower Inflection Point (1999)

Measuring elastic pressure-volume (Pel-V) curves of the respiratory system and the volume recruited by a positive end-expiratory pressure (PEEP) allows one to study the pressure range over which recruitment occurs in acute lung injury (ALI), and to explain how recruitment affects the compliance. Pel-V curves were measured with the low flow inflation technique in 11 patients mechanically ventilated for ALI. Curve I was recorded during inflation from the volume attained after a prolonged expiration (6 s) at PEEP (9.0 +/- 2.2 cm H2O), and Curve II after expiration to the elastic equilibrium volume at zero end-expiratory pressure (ZEEP). By using the end-expiratory volume of the breaths, the curves were aligned on a common volume axis to determine the effect of a single complete expiration. In each patient, Curve II (from ZEEP) was shifted toward lower volumes than Curve I. The volume shift, probably due to derecruitment, was 205 +/- 100 ml at 15 cm H2O (p < 0.01) and 78 +/- 93 ml at 30 cm H2O (p < 0.01); thus, during inflation from ZEEP, the volume deficit was successively regained over a pressure range up to at least 30 cm H2O. At any pressure, compliance was higher on the curve from ZEEP than from PEEP, by 10.0 +/- 8.7 ml/cm H2O at 15 cm H2O (p < 0.01), and by 5.4 +/- 5.5 at 30 cm H2O (p < 0.01). It is concluded that in ALI, a single expiration to ZEEP leads to lung collapse. High compliance during insufflation from ZEEP indicates that lung recruitment happens far above the lower inflection point of the Pel-V curve.
Characteristics of CMA adaptive array for selective fading compensation in digital land mobile radio communications (1991)

Abstract This paper considers CMA adaptive array for the high‐speed TDMA transmission system using GMSK, which is the most ordinary constant‐envelope modulation system in the digital mobile land communication. A computer simulation is executed for the case where a four‐element adaptive array is applied to cope with the selective fading. CMA is employed as the array control algorithm, which is suited to the compensation of the constant‐envelope modulated signal. Assuming a model with two arriving waves, the convergence and the BER performances of CMA are evaluated. It is shown that the BER is improved greatly up to the limit that no more error can be reduced, compared to the case without an adaptive array. It is seen also that the error rate is improved better when the delay difference between the two arriving waves is large. Thus, the proposed method is shown to be an effective technique to cope with selective fading.
Hydrogenation of carboxylic acids by rhenium‐osmium bimetallic catalyst (1990)

Abstract Hydrogenation of carboxylic acids to alcohols at low temperature and under low pressure was achieved by using a new catalyst system, a rhenium‐osmium bimetallic catalyst. The most active catalyst was prepared by the reductionof the corresponding metal oxides with hydrogen in the presence of succinic acid. Decanoic acid was hydrogenated to decanol in high conversions at 25∼100 atm and 100∼120°C. Decane was formed as a by‐product by overreduction of the alcohol. The selectivity of alcohol was improved by the addition of thiophene as a modifier of the catalyst.
Reducing the environmental impact of poultry breeding by genetic selection1 (2012)

Improving the sustainability of poultry production involves limiting its environmental impact and maintaining effectiveness. It has recently been shown that genetic selection on the ability of chickens to digest wheat at 23 d of age can decrease chicken excretion without decreasing BW at this age. The aim of this study was to check whether selection on digestibility modified excretion and growth performance over the whole production cycle. The 2 divergent lines selected for high (D+) and low (D-) apparent metabolizable energy corrected for 0 N balance (AMEn) values were compared with a reference line used at the beginning of the selection experiment (RL) to evaluate the potential excretion improvement that could be expected with such selection. These 3 lines were therefore compared for growth and excretion (raw and relative to feed intake, fresh and dry excreta weights, and moisture content of excreta) from 4 to 53 d. Between 4 and 7 d, 17 and 21 d, and 49 and 53 d, AMEn and N and P excretion rates were also compared between the 3 lines. Furthermore, body composition (breast meat and abdominal fat yields), bone breaking strength, and meat quality traits (lightness, redness, yellowness, and ultimate pH) were compared between lines at 53 d. Over the whole rearing period, D+ birds excreted significantly less fresh and dry excreta (-56 and -61%) than D- and RL birds (-6 and -26%). Similarly, N and P excretion rates of D+ birds were 13% to 30% less than those of D- birds and 12% to 19% less than RL birds, depending on age. These excretion differences may be related to the differential development of the gastrointestinal tract. Differences between lines were already present at 7 d for relative gizzard weight and the weight of the upper to the lower part of the gastrointestinal tract ratio. Anatomic differences were maximum at 23 d for all traits except for relative weight of the duodenum. At slaughter age, BW, breast and fat yields, and meat color did not differ between D+ and RL birds, but D- birds were fatter than D+ and RL birds. Finally, ultimate meat pH was 1% to 2% greater in RL birds than in the D+ and D- lines. In conclusion, this study showed that selection of chickens for AMEn is a possible way to reduce the environmental impact of production over the whole rearing period without a negative impact on growth, body composition, or meat quality.
A NEW LIQUID PHASE FOR GAS CHROMATOGRAPHIC SEPARATIONS OF STEROIDS (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA NEW LIQUID PHASE FOR GAS CHROMATOGRAPHIC SEPARATIONS OF STEROIDSW. J. A. VandenHeuvel, E. O. A. Haahtl, and E. C. HorningCite this: J. Am. Chem. Soc. 1961, 83, 6, 1513–1514Publication Date (Print):March 1, 1961Publication History Published online1 May 2002Published inissue 1 March 1961https://pubs.acs.org/doi/10.1021/ja01467a054https://doi.org/10.1021/ja01467a054research-articleACS PublicationsRequest reuse permissionsArticle Views42Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Steroid regulation of monoamine oxidase activity in the adrenal medulla (1989)

Administration of different steroid hormones in vivo has distinct and specific effects on the MAO activity of the adrenal medulla. In an effort to reconstitute these effects in defined cells, we have isolated endothelial cells and chromaffin cells from the bovine adrenal medulla and tested each cell type for sensitivity to these steroids. As in the intact animal, we found that endothelial cell MAO activity was stimulated 1.5- 2.5-fold by 10 microM progesterone, hydrocortisone, and dexamethasone, inhibited by ca. 50% by 17-alpha-estradiol, but unaffected by testosterone. The type of MAO in the endothelial cells was found to be exclusively of the A type. The chromaffin cells had MAO B exclusively and were inert to treatment with dexamethasone. The mode of action of the various steroids on MAO A activity in endothelial cells seemed to be that of affecting the number of MAO molecules, as binding of [3H]pargyline, an MAO inhibitor, changed in proportion to changes in enzyme activity. Consistently, the kinetic parameters for MAO A showed changes in Vmax but not Km under all conditions. The specificity of steroid action on MAO A activity was also supported by the fact that steroid-induced changes in total cell division ([14C]thymidine incorporation) and total protein synthesis ([14C]leucine incorporation) were seen after changes in MAO A. We conclude that the differential effects of steroids on MAO activity in the intact adrenal medulla can be reproduced in cultured adrenal medullary endothelial cells but not in chromaffin cells. Therefore we suggest that the action of these steroid hormones on the intact adrenal medulla may be restricted to the endothelial cell component of this tissue.
Diastereoselective Synthesis of Chiral (Triazolinylidene)rhodium Complexes Containing an Axis of Chirality (1998)

Deprotonation of chiral triazolium salts 1 and reaction of the resulting nucleophilic carbenes with [(COD)RhCl]2 or [(NBD)RhCl]2 afforded square-planar complexes 2–6 in yields of 65–95%. The complexes contain an axis of chirality and a diastereomeric excess of up to 97% was achieved. The relative and absolute configurations of these complexes were determined by NMR spectroscopic investigations and X-ray structure analysis. The application of the rhodium(COD) complexes as catalysts in an asymmetric hydrosilylation reaction has been examined, resulting in enantiomeric excesses of up to 44%. Similar results were achieved for aromatic and aliphatic ketones and a nonlinear temperature effect (principle of isoinversion) was observed.
Enantioselective Nitrene Transfer to Sulfides Catalyzed by a Chiral Iron Complex (2013)

Iron works: Enantioselective nitrene transfer to sulfide was accomplished by a chiral iron(III)/PyBOX catalyst (see scheme). Various sulfimides were thus obtained in high enantioselectivities and yields. Applications of this protocol to the syntheses of enantioenriched sulfoximines and an epoxide were also demonstrated. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Cathelicidin Antimicrobial Peptides with Reduced Activation of Toll-Like Receptor Signaling Have Potent Bactericidal Activity against Colistin-Resistant Bacteria (2016)

Cathelicidins are antimicrobial peptides that can also increase inflammatory responses. This combination of activities can cause complications in the treatment of bacterial infections despite the pressing need for new antimicrobials. We have identified cathelicidins with decreased activation of inflammatory responses. The peptides kill Gram-negative bacteria at low micromolar concentrations by binding to and perturbing the integrity of the bacterial membrane. The peptides were also engineered to further decrease lysis of human red blood cells. The peptides have activities comparable to those of the polymyxins, a class of antibiotics to which plasmid-borne resistance is rapidly spreading and can kill colistin-resistant bacteria. These peptides are promising candidates for the development of novel antibacterial agents.
Cutaneous wound healing of chronically stressed mice is improved through catecholamines blockade (2010)

Stress impairs cutaneous wound healing; however, it is unclear how beta-adrenoceptors participates in alterations induced by stress on skin wound repair. Therefore, the aim of this study was to investigate the effects of propranolol, a non-selective beta-blocker, administration on cutaneous wound healing of chronically stressed mice. Male mice were spun at 115 rpm for 15 min every hour from three days before wounding until euthanasia. Control animals were not submitted to stress. Stressed and control animals were treated with propranolol dissolved in water; controls received only water. Propranolol administration began one day before wounding and was continued daily until euthanasia. A full-thickness excisional lesion was performed. Seven and fourteen days later, animals were killed, and lesions were formalin-fixed and paraffin-embedded. Sections were stained with hematoxylin-eosin and immunostained against F4/80 to quantify macrophages, alpha-smooth muscle actin to quantify the myofibroblast density and proliferating cell nuclear antigen to quantify the cell proliferation. Furthermore, matrix metalloproteinases (MMP)-2 and MMP-9 activity, nitrite and hydroxyproline levels and tumor necrosis factor-alpha (TNF-alpha) expression were measured in wound. Stress and control + propranolol groups presented a delay in wound contraction, re-epithelialization, F4/80-positive macrophages, neutrophils and mast cells infiltration, cellular proliferation, angiogenesis, myofibroblastic differentiation, MMP-2 and MMP-9 activation and TNF-alpha expression, whereas an increase in the nitrite levels. Stress + propranolol group presented results similar to control group. In conclusion, stress impairs cutaneous wound healing in mice through beta1- adrenoceptors and beta2-adrenoceptors activation.
THE SPECIFICITY OF INTERFERON INDUCTION IN CHICK EMBRYO CELLS BY HELICAL RNA (1969)

Treatment of chick embryo cells growing in culture with rI:rC and many other RNA-like polymers results in the induction of interferon. DEAE dextran is required to facilitate the uptake of the RNA into the cells. Interferon-inducing activity is found with a variety of double-stranded helical polynucleotides, provided that all the sugar residues are ribose. However, the effectiveness of different active polynucleotides at a given concentration varies considerably. The differences in activity among the various polynucleotides do not appear to reflect differences in the rate or amount of uptake into the cells or in the rate of intracellular breakdown. Thehigh degree of specificity of the induction process is consistent with the existence of a specific intracellular receptor site, which may be a protein.
Response of L-929 Fibroblasts, Human Gingival Fibroblasts, and Human Tissue Mast Cells to Various Metal Cations (1995)

Recent data suggest that under certain conditions, various metal cations are released from dental alloys. These ions may produce adverse effects in various cell types in vivo. In this study, the cytopathogenic effects of 13 metal cations on murine L-929 fibroblasts, human gingival fibroblasts, and human tissue mast cells were analyzed in vitro. Several metal cations (dose range, from 0.0033 to 1.0 mmol/L) were found to induce dose-dependent inhibition of 3H-thymidine incorporation into cultured fibroblasts. The rank order of potency (lowest observed effect level, LOEL) for L-929 fibroblasts was: Ag+ > Pt4+ > Co2+ > In3+ > Ga3+ > Au3+ > Cu2+ > Ni2+ > Zn2+ > Pd2+ > Mo5+ > Sn2+ > Cr2+. A similar rank order of potency was obtained for primary human gingival fibroblasts: Pt4+ > Ag+ > Au3+ > In3+ > Ga3+ > Ni2+ > Co2+ > Zn2+ > Cu2+ > Cr2+ > Pd2+ > Mo5+ > Sn2+. In primary human mast cells, Ag+ and Au3+ caused dose-dependent toxic histamine release, whereas the other metal cations were ineffective over the dose range tested. To investigate the mechanism of metal cation-induced effects, we performed DNA as well as electron microscopic analyses on cultured fibroblasts. Both the DNA pattern and the ultrastructure of L-929 cells and gingival fibroblasts after exposure to cytopathogenic metal cations revealed signs of necrosis but no signs of apoptosis. Together, our data provide evidence that various metal cations produce dose-dependent cytopathogenic effects in distinct cell types, including human gingival fibroblasts and human tissue mast cells.(ABSTRACT TRUNCATED AT 250 WORDS)