gebhart/patrepeval huggingface dataset:
Perturbations in Endothelial Dysfunction-Associated Pathways in the Nitrofen-Induced Congenital Diaphragmatic Hernia Model (2017)

Although it is well known that nitrofen induces congenital diaphragmatic hernia (CDH), including CDH-associated lung hypoplasia and pulmonary hypertension (PH) in rodents, the mechanism of pathogenesis remains largely unclear. It has been reported that pulmonary artery (PA) endothelial cell (EC) dysfunction contributes to the development of PH in CDH. Thus, we hypothesized that there is significant alteration of endothelial dysfunction-associated proteins in nitrofen-induced CDH PAs. Pregnant SD rats received either nitrofen or olive oil on gestational day 9.5. The newborn rats were sacrificed and divided into a CDH (n = 81) and a control (n = 23) group. After PA isolation, the expression of PA endothelial dysfunction-associated proteins was assessed on Western blot and immunostaining. We demonstrate that the expression of C-reactive protein and endothelin-1 and its receptors, ETA and ETB, were significantly increased in the CDH PAs. Levels of phosphorylated myosin light chain were significantly elevated, but those of phosphorylated endothelial nitric oxide synthase, caveolin-1, and mechanistic target of rapamycin were significantly decreased in the CDH PAs. In this work, we elucidate alterations in the expression of endothelial dysfunction-associated proteins specific to nitrofen-induced CDH rodent PAs, thereby advancing our understanding of the critical role of endothelial dysfunction-associated pathways in the pathogenesis of nitrofen-induced CDH.
Characterisation of glutathione transferases and glutathione peroxidases in pea (<i>Pisum sativum</i>) (1996)

Glutathione peroxidases (GPOXs) and glutathione transferases, also termed glutathione S ‐transferases (GST, EC 2.5.1.18), with activities toward a range of xenobiotic substrates including herbicides, have been characterized in etiolated pea ( Pisum sativum L. cv. Feltham's First) seedlings. Crude extracts showed high activity toward a range of GST substrates including 1‐chloro‐2,4‐dinitrobenzene (GSTC activity) and the herbicide fluorodifen (GSTF) but low activities toward chloroacetanilides and atrazine. Treatment of the pea seedlings with the herbicide safener dichlormid selectively increased the activity of GSTC and the GST which detoxified atrazine. This induction was restricted to the roots and was not observed with any of the other GST or GPOX activities. In contrast, treatment with CuCl 2 increased GPOX activity in the root but had no effect on any GST activity, while treatment of epicotyls with elicitors of the phytoalexin response increased GST activity toward ethacrynic acid, but had no effect on other GST or GPOX activities. The major enzymes with GSTC, GSTF and GPOX activities were purified from pea epicotyls 3609‐fold, 1431‐fold and 1554‐fold, respectively. During purification by hydrophobic interaction chromatography and affinity chromatography using S‐hexyl‐glutathione as ligand all three activities co‐eluted but could be partially resolved by anion exchange chromatography and gel filtration chromatography. Both GSTC and GPOX had a molecular mass of 48 kDa and their activities were associated with a similar 27.5‐kDa subunit but distinct 29‐kDa subunits. GSTF could be resolved into two isoenzymes with molecular masses of 49.5 and 54 kDa. GSTF activity was associated with a unique 30‐kDa subunit in addition to 27.5‐ and 29‐kDa peptides, suggesting that the two isoenzymes were composed of differing subunits. These results demonstrate that peas contain multiple GST isoenzymes some of which have GPOX activity and that the various activities are differentially responsive to biotic and abiotic stress.
Texas Ranger, April 1949 (2016)

Table of Contents: Firing Line (p. 5) -- Guide book (p. 18) -- Bed Ridden (p. 20) -- Key Chain (p. 22) -- Twisted Tube (p. 24) -- Poetry Possibly (p. 25)
<i>APOL1</i> Risk Variants, Race, and Progression of Chronic Kidney Disease (2013)

Renal risk variants in APOL1 were associated with the higher rates of end-stage renal disease and progression of chronic kidney disease that were observed in black patients as compared with white patients, regardless of diabetes status. (Funded by the National Institute of Diabetes and Digestive and Kidney Diseases and others.).
Intranasal delivery of a bivalent norovirus vaccine formulated in an in situ gelling dry powder (2017)

The global health community is beginning to understand the burden of norovirus-associated disease, which has a significant impact in both developed and developing countries. Norovirus virus like particle (VLP)-based vaccines are currently under development and have been shown to elicit systemic and mucosal immune responses when delivered intranasally. In the present study, we describe the use of a dry powder formulation (GelVac™) with an in situ gelling polysaccharide (GelSite™) extracted from Aloe vera for nasal delivery of a bivalent vaccine formulation containing both GI and GII.4 norovirus VLPs. Dose-ranging studies were performed to identify the optimal antigen dosages based on systemic and mucosal immune responses in guinea pigs and determine any antigenic interference. A dose-dependent increase in systemic and mucosal immunogenicity against each of the VLPs were observed as well as a boosting effect for each VLP after the second dosing. A total antigen dose of ≥50 μg of each GI and GII.4 VLPs was determined to be the maximally immunogenic dose in guinea pigs. The immunogenicity results of this bivalent formulation, taken together with previous work on monovalent GelVac™ norovirus vaccine formulation, provides a basis for future development of this norovirus VLP vaccine.
The role of morphology and crystallographic structure of metal oxides in response of conductometric-type gas sensors (2008)

This review paper discusses the influence of morphology and crystallographic structure on gas-sensing characteristics of metal oxide conductometric-type sensors. The effects of parameters such as film thickness, grain size, agglomeration, porosity, faceting, grain network, surface geometry, and film texture on the main analytical characteristics (absolute magnitude and selectivity of sensor response (S), response time (τres), recovery time (τrec), and temporal stability) of the gas sensor have been analyzed. A comparison of standard polycrystalline sensors and sensors based on one-dimension structures was conducted. It was concluded that the structural parameters of metal oxides are important factors for controlling response parameters of resistive type gas sensors. For example, it was shown that the decrease of thickness, grain size and degree of texture is the best way to decrease time constants of metal oxide sensors. However, it was concluded that there is not universal decision for simultaneous optimization all gas-sensing characteristics. We have to search for a compromise between various engineering approaches because adjusting one design feature may improve one performance metric but considerably degrade another.
A new torque guide wire. (1987)

A new guide wire that provides markedly improved torque control is described. It was compared with a conventional guide wire in two models, one simulating a drainage tube with numerous side holes and one, the biliary tree. The new wire was much more easily controlled than the conventional guide wire and passed through both models significantly faster (P less than .01 and P less than .005). Although some deterioration in control was noted when it was inserted through a catheter in vivo, the new torque-control wire still exhibited a definite improvement over conventional wires in directional control.
Production of human interferons: an overview. (1983)

The world-wide increased demand for human interferons (IFNs), mainly for clinical trials, has led to the development of procedures for production of various types of IFNs. Today, human IFNs: leukocyte, fibroblast, lymphoblastoid, immune and genetically-engineered (recombinant) IFNs are produced in large quantities. Each IFN has its own production methodology based on the characteristics of the producer cells, IFN inducer and the IFN itself. Various equipment is being used for production of crude IFN, its concentration and purification. Various IFN preparations are being supplied in different specific activities. Final IFN preparations are tested according to strict regulations established by Health authorities.
Significance of micrometastasis in bone marrow and blood of operable breast cancer patients: research tool or clinical application? (2007)

Approximately 25% of breast cancer patients without lymph node metastases develop systemic relapse. A growing body of data supports the notion that hematogenous dissemination of breast cancer cells occurs independently of lymphatic spread of disease; however, current clinical practice does not involve routine analysis of circulating or disseminated cells. Recent studies have documented that both circulating tumor cells (CTCs) within the blood and disseminated tumor cells (DTCs) in bone marrow can be identified using a variety of techniques. It is now clear that the presence of DTCs correlates with subsequent development of clinically evident bone metastases, and a worse outcome from breast cancer. While there are data identifying prognostic significance of CTCs in patients with metastatic breast cancer, there are few data regarding CTCs in operable patients. Factors such as presence of a cancer stem cell phenotype and/or certain microenvironmental conditions are involved in the establishment of distant metastases from a primary breast cancer, emphasizing the need for further studies within this area. The purpose of this report is to review the data regarding CTCs and DTCs in patients with operable breast cancer.
DNA fingerprinting in domestic animals using four different minisatellite probes (1988)

Four probes known to allow DNA fingerprinting in the human (M13, Jeffreys' core sequence, the human alpha globin hypervariable region [HVR], and a mouse probe related to the Drosophila Per gene) were checked for their ability to reveal "genetic bar codes" in cattle, horses, pigs, dogs, chickens, and a European cyprinid fish, the barbel (Barbus barbus L.). Individual-specific patterns were obtained in cattle using M13, Jeffreys' core sequence, and the alpha globin HVR, in horses, dogs, and pigs using M13, Jeffreys' core sequence, and the Per probe, and in chicken and fish using the four different probes. Although we observed a considerable heterogeneity in the extent of interindividual variation, depending on the particular probe-species combination, the fingerprints are polymorphic enough to be used efficiently in animal identification, paternity testing, and as a source of genetic markers for linkage analysis. These markers should substantially accelerate the mapping of genes affecting economically important traits.
Protective Effects of Murine Monoclonal Antibodies in Experimental Septicemia: E. coli Antibodies Protect Against Different Serotypes of E. coli (1989)

Murine monoclonal antibodies that bind outer membrane antigens of the J5 mutant of Escherichia coli O111:B4 were derived from spleen cells of BALB/c mice immunized with killed whole cells and boosted with lipopolysaccharide (LPS) and LPS-associated proteins. Seven hybridomas were selected for their reactivity against the J5 LPS; they cross-reacted with O111, O55, O127, and O128 E. coli LPS. One (B7B3) also reacted with the Serratia marcescens LPS and Klebsiella pneumoniae lipid A. A protective effect was obtained with D6B4 antibody in a lethal endotoxemia model induced by LPS from O111, O127, and O128 E. coli serotypes in D-galactosamine-sensitized mice. D6B4 and D6B3 antibodies protected mice infected with E. coli O111:B4, when administered before infection. The D6B4 antibody was also protective when administered after infection. The antibodies D6B3 and D4B5 were protective in heterologous infection induced by E. coli O2:K1.
Amplification of autoimmune disease by infection. (2005)

Reports of infection with certain chronic persistent microbes (herpesviruses or Chlamydiae) in human autoimmune diseases are consistent with the hypothesis that these microbes are reactivated in the setting of immunodeficiency and often target the site of autoimmune inflammation. New experimental animal models demonstrate the principle. A herpesvirus or Chlamydia species can be used to infect mice with induced transient autoimmune diseases. This results in increased disease severity and even relapse. The evidence suggests that the organisms are specifically imported to the inflammatory sites and cause further tissue destruction, especially when the host is immunosuppressed. We review the evidence for the amplification of autoimmune inflammatory disease by microbial infection, which may be a general mechanism applicable to many human diseases. We suggest that patients with autoimmune disorders receiving immunosuppressing drugs should benefit from preventive antiviral therapy.
Predicting clicks: CTR estimation of advertisements using Logistic Regression classifier (2015)

Search engine advertising in the present day is a pronounced component of the Web. Choosing the appropriate and relevant ad for a particular query and positioning of the ad critically impacts the probability of being noticed and clicked. It also strategically impacts the revenue, the search engine shall generate from a particular Ad. Needless to say, showing the user an Ad that is relevant to his/her need greatly improves users satisfaction. For all the aforesaid reasons, its of utmost importance to correctly determine the click-through rate (CTR) of ads in a system. For frequently appearing ads, CTR is empirically measurable, but for the new ads, other means have to be devised. In this paper we propose and establish a model to predict the CTRs of advertisements adopting Logistic Regression as the effective framework for representing and constructing conditions and vulnerabilities among variables. Logistic Regression is a type of probabilistic statistical classification model that predicts a binary response from a binary predictor, based on one or more predictor variables. Advertisements that have the most elevated to be clicked are chosen using supervised machine learning calculation. We tested Logistic Regression algorithm on a one week advertisement data of size around 25 GB by considering position and impression as predictor variables. Using this prescribed model we were able to achieve around 90% accuracy for CTR estimation.
On the feasibility of beamforming in millimeter wave communication systems with multiple antenna arrays (2014)

The use of the millimeter (mm) wave spectrum for next generation (5G) mobile communication has gained significant attention recently. The small carrier wavelengths at mmwave frequencies enable synthesis of compact antenna arrays, providing beamforming gains that compensate the increased propagation losses. In this work, we investigate the feasibility of employing multiple antenna arrays (at the transmitter and/or receiver) to obtain diversity/multiplexing gains, where each of the arrays is capable of beamforming independently. Considering a codebook based beamforming system (the set of possible beamforming directions is fixed a priori, e.g., to facilitate limited feedback), we observe that the complexity of jointly optimizing the beamforming directions across the multiple arrays is highly prohibitive, even for very reasonable system parameters. To overcome this bottleneck, we develop two complementary approaches, premised on the sparse multipath structure of the mmwave channel. Specifically, we reduce the complexity of the joint beamforming optimization problem by restricting attention to a small set of candidate directions, based on: (a) computation of a novel spatial effective power metric, and (b) estimation of the channel's angles of arrival. Our methods enable a drastic reduction of the optimization search space (a factor of 100 reduction), while delivering close to optimal performance, thereby indicating the potential feasibility of achieving diversity and multiplexing gains in mmwave systems.
Early Lymphatic Tumor Cell Dissemination in Pancreatic Cancer (1997)

Tumor relapse occurs frequently in patients with ductal pancreatic head cancer despite the absence of residual tumor detectable at primary surgery. Therefore it has to be assumed that current tumor staging procedures fail to detect minimal amounts of disseminated tumor cells present in secondary organs, which might be the seed for subsequent metastatic relapse. We evaluated lymph nodes from 18 patients without overt metastases who had undergone radical tumor resection (R0 resection). Lymph nodes judged as "tumor-free" by routine histopathology were further examined for the presence of single tumor cells using immunohistochemistry with the antiepithelial monoclonal antibody Ber-EP4. Sixteen of 37 "tumor-free" lymph nodes (43.2%), obtained from 13 of 18 patients (72.2%), displayed Ber-EP4+ tumor cells. Twelve of these 18 patients presented at pT2 stage. Nine of 12 patients (75%) staged as pN0 had these cells. Two of six pN1 patients had no Ber-EP4+ in histopathologically tumor-free lymph nodes. Using multivariate Cox's regression analysis, the presence of Ber-EP4+ cells in "tumor-free" lymph nodes was an independent factor for a significantly reduced relapse-free survival (p = 0.006) and overall survival (p = 0.01). Remarkably, all patients who were restaged as lymph node negative by both histopathology and immunohistochemistry survived the observation period without recurrence. The frequent occurrence of disseminated tumor cells in patients with pancreatic cancer and their prognostic impact support the need for a refined staging system of excised lymph nodes, which should include immunohistochemical examination. Thus patients with a minimal residual tumor load who might benefit from an adjuvant therapy could be selected.
A Simplified Two-Stage Equalizer with a Reduced Number of Multiplications for Data Transmission over Voiceband Telephone Links (1984)

The paper presents an equalization scheme based on the two-stage equalizer originally introduced by Proakis. Mostly additions are required for implementation of the equalizer structure and the adaptation algorithm. The equalizer can be applied to data transmission Systems which use four-phase modulation, especially in 1200/2400 bit/s modems, thus replacing the fixed equalizer recommended by CCITT. The results presented in the paper deal with the analog and digital implementation of the proposed equalizer. Its performance is compared to the performance of the conventional transversal equalizer, a decision-feedback equalizer, an ideal linear canceller, and an ideal QPSK system.
Calcium-Dependent Ligand Binding and G-protein Signaling of Family B GPCR Parathyroid Hormone 1 Receptor Purified in Nanodiscs (2012)

GPCRs mediate intracellular signaling upon external stimuli, making them ideal drug targets. However, little is known about their activation mechanisms due to the difficulty in purification. Here, we introduce a method to purify GPCRs in nanodiscs, which incorporates GPCRs into lipid bilayers immediately after membrane solubilization, followed by single-step purification. Using this approach, we purified a family B GPCR, parathyroid hormone 1 receptor (PTH1R), which regulates calcium and phosphate homeostasis and is a drug target for osteoporosis. We demonstrated that the purified PTH1R in nanodiscs can bind to PTH(1-34) and activate G protein. We also observed that Ca(2+) is a weak agonist of PTH1R, and Ca(2+) in millimolar concentration can switch PTH(1-34) from an inverse agonist to an agonist. Hence, our results show that nanodiscs are a viable vehicle for GPCR purification, enabling studies of GPCRs under precise experimental conditions without interference from other cellular or membrane components.
Transcranial Electrical Neuromodulation Based on the Reciprocity Principle (2016)

A key challenge in multi-electrode transcranial electrical stimulation (TES) or transcranial direct current stimulation (tDCS) is to find a current injection pattern that delivers the necessary current density at a target and minimizes it in the rest of the head, which is mathematically modeled as an optimization problem. Such an optimization with the Least Squares (LS) or Linearly Constrained Minimum Variance (LCMV) algorithms is generally computationally expensive and requires multiple independent current sources. Based on the reciprocity principle in electroencephalography (EEG) and TES, it could be possible to find the optimal TES patterns quickly whenever the solution of the forward EEG problem is available for a brain region of interest. Here, we investigate the reciprocity principle as a guideline for finding optimal current injection patterns in TES that comply with safety constraints. We define four different trial cortical targets in a detailed seven-tissue finite element head model, and analyze the performance of the reciprocity family of TES methods in terms of electrode density, targeting error, focality, intensity, and directionality using the LS and LCMV solutions as the reference standards. It is found that the reciprocity algorithms show good performance comparable to the LCMV and LS solutions. Comparing the 128 and 256 electrode cases, we found that use of greater electrode density improves focality, directionality, and intensity parameters. The results show that reciprocity principle can be used to quickly determine optimal current injection patterns in TES and help to simplify TES protocols that are consistent with hardware and software availability and with safety constraints.
Innovations in thermoplastic die attach adhesives for microelectronic packaging (2002)

This paper reviews the traditional use of thermoset (epoxy) polymer adhesives and highlights some of the limitations for current microelectronic applications. The development of thermoplastic materials offers many advantages in material options in terms of form and processing. The past two or three years has seen a rapid uptake of these new thermoplastic adhesive materials for attaching large area silicon to a wide range of substrate and package materials. Furthermore, new innovative concepts in die attach, ranging from dry adhesive preform, ribbon and extruded rod processes have been developed in both the USA and Far East, and this paper outlines the techniques used for some of these process options. An outline of some equipment developments to use these new adhesive technologies especially for die attach is given and summary data on "end user" case histories is also presented. The paper concludes with a prediction for the future in that even more novel thermoplastic adhesive materials will be developed, offering a range of both isotropic and anisotropic form options.