gebhart/patrepeval huggingface dataset:
Rostafuroxin: an ouabain antagonist that corrects renal and vascular Na<sup>+</sup>-K<sup>+</sup>- ATPase alterations in ouabain and adducin-dependent hypertension (2006)

The genetic and environmental heterogeneity of essential hypertension is responsible for the individual variability of antihypertensive therapy. An understanding of the molecular mechanisms underlying hypertension and related organ complications is a key aspect for developing new, effective, and safe antihypertensive agents able to cure the cause of the disease. Two mechanisms, among others, are involved in determining the abnormalities of tubular Na+ reabsorption observed in essential hypertension: the polymorphism of the cytoskeletal protein alpha-adducin and the increased circulating levels of endogenous ouabain (EO). Both lead to increased activity and expression of the renal Na+-K+ pump, the driving force for tubular Na transport. Morphological and functional vascular alterations have also been associated with EO. Rostafuroxin (PST 2238) is a new oral antihypertensive agent able to selectively antagonize EO, adducin pressor, and molecular effects. It is endowed with high potency and efficacy in reducing blood pressure and preventing organ hypertrophy in animal models representative of both adducin and EO mechanisms. At molecular level, in the kidney, Rostafuroxin antagonizes EO triggering of the Src-epidermal growth factor receptor (EGFr)-dependent signaling pathway leading to renal Na+-K+ pump, and ERK tyrosine phosphorylation and activation. In the vasculature, it normalizes the increased myogenic tone caused by nanomolar ouabain. A very high safety ratio and an absence of interaction with other mechanisms involved in blood pressure regulation, together with initial evidence of high tolerability and efficacy in hypertensive patients, indicate Rostafuroxin as the first example of a new class of antihypertensive agents designed to antagonize adducin and EO-hypertensive mechanisms.
Fast frequency acquisition via adaptive least squares algorithm (1986)

A new least squares algorithm is proposed and investigated for fast frequency and phase acquisition of sinusoids in the presence of noise. This algorithm is a special case of more general, adaptive parameter-estimation techniques. The advantages of the algorithms are their conceptual simplicity, flexibility and applicability to general situations. For example, the frequency to be acquired can be time varying, and the noise can be nonGaussian, nonstationary and colored. As the proposed algorithm can be made recursive in the number of observations, it is not necessary to have a priori knowledge of the received signal-to-noise ratio or to specify the measurement time. This would be required for batch processing techniques, such as the fast Fourier transform (FFT). The proposed algorithm improves the frequency estimate on a recursive basis as more and more observations are obtained. When the algorithm is applied in real time, it has the extra advantage that the observations need not be stored. The algorithm also yields a real time confidence measure as to the accuracy of the estimator.
Surface differentiation by parametric modeling of infrared intensity scans (2005)

We differentiate surfaces with different properties with simple low-cost IR emitters and detectors in a location-invariant manner. The intensity readings obtained with such sensors are highly dependent on the location and properties of the surface, which complicates the differentiation and localization process. Our approach, which models IR intensity scans parametrically, can distinguish different surfaces independent of their positions. Once the surface type is identified, its position (r,) can also be estimated. The method is verified experimentally with wood; Styrofoam packaging material; white painted matte wall; white and black cloth; and white, brown, and violet paper. A correct differentiation rate of 100% is achieved for six surfaces, and the surfaces are localized within absolute range and azimuth errors of 0.2 cm and 1.1 deg, respectively. The differentiation rate decreases to 86% for seven surfaces and to 73% for eight surfaces. The method demonstrated shows that simple IR sensors, when coupled with appropriate signal processing, can be used to recognize different types of surfaces in a location-invariant manner.
MADS-Box Gene Diversity in Seed Plants 300 Million Years Ago (2000)

MADS-box genes encode a family of transcription factors which control diverse developmental processes in flowering plants ranging from root development to flower and fruit development. Through phylogeny reconstructions, most of these genes can be subdivided into defined monophyletic gene clades whose members share similar expression patterns and functions. Therefore, the establishment of the diversity of gene clades was probably an important event in land plant evolution. In order to determine when these clades originated, we isolated cDNAs of 19 different MADS-box genes from Gnetum gnemon, a gymnosperm model species and thus a representative of the sister group of the angiosperms. Phylogeny reconstructions involving all published MADS-box genes were then used to identify gene clades containing putative orthologs from both angiosperm and gymnosperm lineages. Thus, the minimal number of MADS-box genes that were already present in the last common ancestor of extant gymnosperms and angiosperms was determined. Comparative expression studies involving pairs of putatively orthologous genes revealed a diversity of patterns that has been largely conserved since the time when the angiosperm and gymnosperm lineages separated. Taken together, our data suggest that there were already at least seven different MADS-box genes present at the base of extant seed plants about 300 MYA. These genes were probably already quite diverse in terms of both sequence and function. In addition, our data demonstrate that the MADS-box gene families of extant gymnosperms and angiosperms are of similar complexities.
Social security fraud, accountability and taming wicked problems (2012)

Background to the study Originally briefed from a heuristic from Legal Aid re types of cases prosecuted and 'cost'. Soon apparent that a typical cost benefit analysis was not only a flawed approach but inherently misleading. Mindful that arguing over 'numbers' was not going to approach the problem from a social accountability perspective. Framed as a Wicked Problem within the Whole-of-Government literature.
Now This/Editorial: Open (White) House (1993)

The White House Communications Office is distributing press releases on an experimental system developed during the Clinton campaign at the Artificial Intelligence Laboratory at the Massachusetts Institute of Technology.
Interactive simulation of surgical needle insertion and steering (2009)

We present algorithms for simulating and visualizing the insertion and steering of needles through deformable tissues for surgical training and planning. Needle insertion is an essential component of many clinical procedures such as biopsies, injections, neurosurgery, and brachytherapy cancer treatment. The success of these procedures depends on accurate guidance of the needle tip to a clinical target while avoiding vital tissues. Needle insertion deforms body tissues, making accurate placement difficult. Our interactive needle insertion simulator models the coupling between a steerable needle and deformable tissue. We introduce (1) a novel algorithm for local remeshing that quickly enforces the conformity of a tetrahedral mesh to a curvilinear needle path, enabling accurate computation of contact forces, (2) an efficient method for coupling a 3D finite element simulation with a 1D inextensible rod with stick-slip friction, and (3) optimizations that reduce the computation time for physically based simulations. We can realistically and interactively simulate needle insertion into a prostate mesh of 13,375 tetrahedra and 2,763 vertices at a 25 Hz frame rate on an 8-core 3.0 GHz Intel Xeon PC. The simulation models prostate brachytherapy with needles of varying stiffness, steering needles around obstacles, and supports motion planning for robotic needle insertion. We evaluate the accuracy of the simulation by comparing against real-world experiments in which flexible, steerable needles were inserted into gel tissue phantoms.
LASER INDUCED FORWARD TRANSFER FOR MATERIALS PATTERNING (2011)

Today, a strong research effort is directed towards the development and integration of new materials, especially of polymers and biomolecules which are essential in pharmaceutical, bioengineering, and sensing applications. In fact, patterning of organic and inorganic materials, in particular enabling precise positioning of different materials with nano and micro scale resolution over large areas, is the main demand in the development of next generation sensors, biosensors, and microarray chip devices.<br />In this study the application of laser induced forward transfer for printing of polymers in solid phase, and biomolecules e.g. liposomes and DNA in liquid phase is shown.
Ray Tracing And Global Illumination (2003)

In order to represent real-world images with a computer, a program has to relate three-dimensional images on a two-dimensional monitor screen. Several ways of doing this exist with varying degrees of realism. One of the most successful methods can be grouped in a "screen-to-world method" of viewing, which is also known as "ray-tracing."This computer graphics technology simulates light rays within a 3D environment. Since light rays have predictable physical properties, the raytracing algorithm can attempt to calculate the exact coloring of each ray/object intersection at any given pixel. Advanced levels of ray tracing allow light rays to bounce from object to object, mimicking what they do in real life."Local illumination" represents the basic form of ray tracing. It only takes into account the relationship between light sources and a single object, but does not consider the effects that result from the presence of multiple objects. For instance, a light source can be intersected by another surface and therefore be obscured to any point behind that surface. Similarly, light can be contributed not by a light source, but by a reflection of light from some other object. The local illumination model does not visually show this reflection of light. Therefore, special techniques have to be used to represent these effects. In real life there are often multiple sources of light and multiple reflecting objects that interact with each other in many ways."Global illumination," the more advanced form of ray tracing, adds to the local model by reflecting light from surrounding surfaces to the object. A global illumination model is more comprehensive, more physically correct, and it produces more realistic images.Ray tracing is an essential subject when it comes to computer graphics. It combines issues of efficiency and realism, thus finding a favorable balance of the time and effort involved to make realistic three dimensional images. In the process of researching the many different ways of implementing a ray tracer, the study began with local illumination and graduated to global illumination, using some pre-established techniques and the development of new techniques.
Rigid fluid (2004)

We present the Rigid Fluid method, a technique for animating the interplay between rigid bodies and viscous incompressible fluid with free surfaces. We use distributed Lagrange multipliers to ensure two-way coupling that generates realistic motion for both the solid objects and the fluid as they interact with one another. We call our method the rigid fluid method because the simulator treats the rigid objects as if they were made of fluid. The rigidity of such an object is maintained by identifying the region of the velocity field that is inside the object and constraining those velocities to be rigid body motion. The rigid fluid method is straightforward to implement, incurs very little computational overhead, and can be added as a bridge between current fluid simulators and rigid body solvers. Many solid objects of different densities ( e.g. , wood or lead) can be combined in the same animation.
Locanyms: Towards Privacy-Preserving Location-Based Services (2012)

International audience
Column Liquid Chromatography: Equipment and Instrumentation (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTColumn Liquid Chromatography: Equipment and InstrumentationCarsten A. Bruckner, Marc D. Foster, Lawrence R. Lima, Robert E. Synovec, Richard J. Berman, Curtiss N. Renn, and Edward L. JohnsonCite this: Anal. Chem. 1994, 66, 12, 1–16Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/ac00084a001https://doi.org/10.1021/ac00084a001research-articleACS PublicationsRequest reuse permissionsArticle Views233Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Influence of globin structure on the state of the heme. I. Human deoxyhemoglobin (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInfluence of globin structure on the state of the heme. I. Human deoxyhemoglobinMax F. Perutz, Jane E. Ladner, Sanford R. Simon, and Chien HoCite this: Biochemistry 1974, 13, 10, 2163–2173Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 May 1974https://pubs.acs.org/doi/10.1021/bi00707a026https://doi.org/10.1021/bi00707a026research-articleACS PublicationsRequest reuse permissionsArticle Views563Altmetric-Citations225LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Maturation Environment and Impact on Subsequent Developmental Competence of Bovine Oocytes (2013)

In vitro production (IVP) of bovine embryos has been improved immensely throughout the last decades. Nevertheless, embryos generated in vitro still differ from their in vivo-produced counterparts. It is possible to achieve blastocyst rates of up to 70% if in vivo-matured oocytes are used. In contrast, if oocytes are matured in vitro, blastocyst rates are only half that of those matured in vivo. This rather limited success may be attributed to the heterogeneous population of oocytes which are normally retrieved from follicles of 3-8 mm rather than from preovulatory follicles. In contrast to the in vivo-ovulated oocyte, these oocytes lack development up to the preovulatory stage and are matured in vitro. Therefore, much effort has been devoted to the establishment of non-invasive and non-perturbing means for selecting the most competent oocytes, for example the extensiveness and compactness of the cumulus-corona investment and the granulation of the ooplasm. In vitro culture (IVC) conditions have been enhanced in the last few years, mainly by adjustment of media formulations, whereas the in vitro maturation (IVM) protocols stay invariable. Consequently, maintaining or mimicking the in vivo situation in vitro will aid to improve the quality and developmental competence of the resulting matured oocyte. The scope of this review is to give an overview of the current situation of in vitro maturation of mammalian oocytes with emphasis on the bovine species. Special attention has been paid to the in vivo situation in the follicle and how a better understanding of these intrafollicular factors will aid to improve the in vitro maturation conditions.
Review of the Pharmacological and Clinical Profile of Rimcazole (2004)

Rimcazole is a carbazole derivative that acts in part as a sigma receptor antagonist. Wellcome Research Laboratories introduced this compound during the 1980s when it was hypothesized to be a novel antipsychotic with an improved side effect profile. However, subsequent clinical trials demonstrated that rimcazole lacked efficacy in schizophrenic patients and it is now primarily used as an experimental tool. In addition to its actions as a sigma receptor antagonist, rimcazole also has high affinity for dopamine transporters, and in recent years it has served as a lead compound for the development of novel dopamine transporter ligands. Although rimcazole cannot be considered a selective ligand for sigma receptors, the recent development of other selective agonists and antagonists for sigma receptors have aided in clarifying the involvement of these receptors in the actions of rimcazole. Many of the physiological and behavioral effects of rimcazole can in fact be ascribed to its action as a sigma receptor antagonist, although there are exceptions. Rimcazole is likely to have a continued role in elucidating sigma receptor function in either in vitro or in vivo systems where sigma receptor-mediated effects can be studied independently of the influence of dopamine and serotonin transporters.
Deleting multiple lytic genes enhances biomass yield and production of recombinant proteins by (2014)

Bacillus subtilis is widely used in agriculture and industrial biotechnology; however, cell autolysis significantly decreases its yield in liquid cultures. Numerous factors mediate the lysis of B. subtilis, such as cannibalism factors, prophages, and peptidoglycan (PG) hydrolases. The aim of this work was to use molecular genetic techniques to develop a new strategy to prevent cell lysis and enhance biomass as well as the production of recombinant proteins. Five genes or genetic elements representing three different functional categories were studied as follows: lytC encoding PG hydrolases, the prophage genes xpf and yqxG-yqxH-cwlA (yGlA), and skfA and sdpC that encode cannibalism factors. Cell lysis was reduced and biomass was enhanced by deleting individually skfA, sdpC, xpf, and lytC. We constructed the multiple deletion mutant LM2531 (skfA sdpC lytC xpf) and found that after 4 h of culture, its biomass yield was significantly increased compared with that of prototypical B. subtilis 168 (wild-type) strain and that 15% and 92% of the cells were lysed in cultures of LM2531 and wild-type, respectively. Moreover, two expression vectors were constructed for producing recombinant proteins (β-galactosidase and nattokinase) under the control of the P43 promoter. Cultures of LM2531 and wild-type transformants produced 13741 U/ml and 7991 U/ml of intracellular β-galactosidase, respectively (1.72-fold increase). Further, the level of secreted nattokinase produced by strain LM2531 increased by 2.6-fold compared with wild-type (5226 IU/ml vs. 2028 IU/ml, respectively). Our novel, systematic multigene deletion approach designed to inhibit cell lysis significantly increased the biomass yield and the production of recombinant proteins by B. subtili s. These findings show promise for guiding efforts to manipulate the genomes of other B. subtilis strains that are used for industrial purposes.
Evaluation of stimulus parameters and electrode geometry for an effective suprachoroidal retinal prosthesis (2010)

Several approaches have been proposed for placement of retinal prostheses: epiretinal, subretinal and suprachoroidal. We aimed to systematically evaluate the effectiveness of varying a range of stimulus parameters and electrode geometry for a suprachoroidal electrode array, using cortical evoked responses to monopolar electrical stimulation in cats. Our results indicate that charge thresholds were not dependent on electrode size, pulse widths or position of the return electrode tested, but were dependent on the number of sites stimulated in parallel. Further, we found that the combination of monopolar stimulation with large diameter electrodes, wide pulse widths and parallel stimulation minimized the voltage requirements for stimulation. These results provide useful insights for the design specifications of a low voltage suprachoroidal stimulator.
Three-Dimensional Porous Metal–Metalloporphyrin Framework Consisting of Nanoscopic Polyhedral Cages (2011)

An unprecedented nanoscopic polyhedral cage-containing metal-metalloporphyrin framework, MMPF-1, has been constructed from a custom-designed porphyrin ligand, 5,15-bis(3,5-dicarboxyphenyl)porphine, that links Cu(2)(carboxylate)(4) moieties. A high density of 16 open copper sites confined within a nanoscopic polyhedral cage has been achieved, and the packing of the porphyrin cages via an "ABAB" pattern affords MMPF-1 ultramicropores which render it selective toward adsorption of H(2) and O(2) over N(2), and CO(2) over CH(4).
Application of Recess Vaned Casing Treatment to Axial Flow Fans (1990)

The effect of applying a vaned recessed casing treatment to a single-stage axial flow fan has been investigated. The influences of the axial position of the recess relative to the rotor leading edge and other geometric modifications of the vane passage have been examined. Significant improvements in stall margin were observed without (in some builds) loss in peak efficiency. Slow and fast frequency response yawmeter probes have been used in the study to examine both the steady flow conditions and the unsteady flow caused by rotating stall.