gebhart/patrepeval huggingface dataset:
Identification of the <i>tliDEF</i> ABC Transporter Specific for Lipase in <i>Pseudomonas fluorescens</i> SIK W1 (1999)

Pseudomonas fluorescens, a gram-negative psychrotrophic bacterium, secretes a thermostable lipase into the extracellular medium. In our previous study, the lipase of P. fluorescens SIK W1 was cloned and expressed in Escherichia coli, but it accumulated as inactive inclusion bodies. Amino acid sequence analysis of the lipase revealed a potential C-terminal targeting sequence recognized by the ATP-binding cassette (ABC) transporter. The genetic loci around the lipase gene were searched, and a secretory gene was identified. Nucleotide sequencing of an 8.5-kb DNA fragment revealed three components of the ABC transporter, tliD, tliE, and tliF, upstream of the lipase gene, tliA. In addition, genes encoding a protease and a protease inhibitor were located upstream of tliDEF. tliDEF showed high similarity to ABC transporters of Pseudomonas aeruginosa alkaline protease, Erwinia chrysanthemi protease, Serratia marcescens lipase, and Pseudomonas fluorescens CY091 protease. tliDEF and the lipase structural gene in a single operon were sufficient for E. coli cells to secrete the lipase. In addition, E. coli harboring the lipase gene secreted the lipase by complementation of tliDEF in a different plasmid. The ABC transporter of P. fluorescens was optimally functional at 20 and 25 degrees C, while the ABC transporter, aprD, aprE, and aprF, of P. aeruginosa secreted the lipase irrespective of temperature between 20 and 37 degrees C. These results demonstrated that the lipase is secreted by the P. fluorescens SIK W1 ABC transporter, which is organized as an operon with tliA, and that its secretory function is temperature dependent.
Mutagenesis of <i>Escherichia coli</i>: a method for determining mutagenic specificity by analysis of tRNA suppressors (1992)

A method for estimating mutagenic specificity in Escherichia coli (argE3, hisG4, thr-1, supE44), based upon the isolation of Arg+ or His+ revertants and identification of tRNA suppressors, is described. The method gives an insight not only into mutagenic pathways but also into the functioning of tRNA. With N-methyl-N'-nitro-N-nitrosoguanidine, 98% of mutations are GC----AT transitions. With N4-hydroxycytidine, 100% are AT----GC transitions. With hydroxylamine, apart from GC----AT transitions, approximately 30% of Arg+ revertants are formed by GC (or AT)----TA transversions. When the chemistry of the mutagenic attack is known, the method allows us to discriminate whether mutations occur on the transcribed or non-transcribed strands of DNA. It has been found that reversion of argE3 to Arg+ is a better monitor of mutagenic pathways than reversion of hisG4 to His+.
Morphometric Analysis of the Lung in Bronchopulmonary Dysplasia (1991)

We studied lung development in children with or without bronchopulmonary dysplasia (BPD) using light microscopic morphometry and thick lung sections stained for elastic fibers. One lung was obtained at autopsy from each of eight patients with BPD (ages, 2 to 28 months) and six children (ages, 5 days to 51 months) who died without lung disease. Patients with BPD demonstrated severe somatic growth retardation and had reduced lung volumes with abnormal lobar volume proportions. In the central bronchi mean volume proportion of glands and smooth muscle was increased in BPD. Bronchiolar density was also increased, but it tended to normalize with advancing age. Mean bronchiolar diameter was slightly smaller in BPD, and bronchiolar smooth muscle hypertrophy was a constant histologic feature. The most striking change, however, was noted in alveolar structure and development. Total alveolar number was severely decreased in patients with BPD compared with that in control subjects, and there was little evidence of compensatory alveolar development with increasing age. Lung internal surface area was correspondingly reduced, and mean linear intercept was increased. Sections stained for elastic tissue demonstrated in the patients with BPD a simplified acinar structure with thickened, tortuous, and irregularly distributed alveolar elastic fibers. We conclude that in severe, fatal BPD there is marked impairment of lung development with alveolar hypoplasia and reduced internal surface area. In addition, bronchial and bronchiolar smooth muscle hypertrophy and bronchial gland hyperplasia may be important contributing factors to airflow limitation.
ChemInform Abstract: 4,5,6,7‐TETRAIODO‐3‐BENZALPHTHALIDES AND RELATED COMPOUNDS (1981)

Abstract Tetraiodphthalsäureanhydrid (I) reagiert mit den Phenylessigsäuren (II) zu den Phthaliden (III), die wie im Formelschema angegeben, zu den Tetraiodverbindungen (IV), (V), (VII) und zu dimeren Phthaliden (VIII) umgesetzt werden.
Cellular Response to Diesel Exhaust Particles Strongly Depends on the Exposure Method (2008)

In vitro exposure to aerosols at the air-liquid interface (ALI) preserves the physical and chemical characteristics of aerosol particles. Although frequently described as being a more physiologic exposure method, ALI exposure has not been directly compared with conventional in vitro exposures where the particles are suspended in medium. We exposed immortalized human bronchial epithelial cells (16HBE14o) to aerosolized diesel exhaust particles at the ALI and to suspensions of collected particles. The response of the cells was determined from measurements of the cell viability and interleukin-8 (IL-8) secretion. The deposited size distribution at the cell surface was measured with transmission electron microscopy to obtain a dose for the ALI exposure. Although exposure by either method caused a slight decrease in cell viability and induced IL-8 secretion, the response to ALI exposure occurred at doses several orders of magnitude lower than exposure to particles in suspension. The most likely sources for the different dose responses are the artifacts introduced during the collection and resuspension of particles for conventional suspension exposures. The number concentration of particles deposited at the ALI is similar to the modeled deposition in the tracheal-bronchial region in a human lung, but the ALI size distribution is skewed toward particles larger than those deposited in the lung.
Regulation of the biosynthesis of cephamycin C by <i>Streptomyces lactamdurans</i> (1984)

Conference Article| October 01 1984 Regulation of the biosynthesis of cephamycin C by Streptomyces lactamdurans JESÚS CORTÉS; JESÚS CORTÉS 1Departamento de Microbiología, Facultad de Biología, Universidad de León, León, Spain Search for other works by this author on: This Site PubMed Google Scholar PALOMA LIRAS; PALOMA LIRAS 1Departamento de Microbiología, Facultad de Biología, Universidad de León, León, Spain Search for other works by this author on: This Site PubMed Google Scholar JOSÉ M. CASTRO; JOSÉ M. CASTRO 1Departamento de Microbiología, Facultad de Biología, Universidad de León, León, Spain Search for other works by this author on: This Site PubMed Google Scholar JORGE ROMERO; JORGE ROMERO 1Departamento de Microbiología, Facultad de Biología, Universidad de León, León, Spain Search for other works by this author on: This Site PubMed Google Scholar JUAN F. MARTÍN JUAN F. MARTÍN 1Departamento de Microbiología, Facultad de Biología, Universidad de León, León, Spain Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1984) 12 (5): 863–864. https://doi.org/10.1042/bst0120863a Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation JESÚS CORTÉS, PALOMA LIRAS, JOSÉ M. CASTRO, JORGE ROMERO, JUAN F. MARTÍN; Regulation of the biosynthesis of cephamycin C by Streptomyces lactamdurans. Biochem Soc Trans 1 October 1984; 12 (5): 863–864. doi: https://doi.org/10.1042/bst0120863a Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: Mops, 4-morpholinepropanesulphonic acid This content is only available as a PDF. © 1984 Biochemical Society1984 Article PDF first page preview Close Modal You do not currently have access to this content.
THE COMPLETE AMINO ACID SEQUENCE OF HUMAN FACTOR V (1987)

cDNA clones encoding human factor V have been isolated and sequenced. The cDNA sequence of factor V obtained from overlapping clones includes a 6672 bp coding region, a 90 bp 5'-untranslated region and a 163 bp 3’-untranslated region including a poly-A tail. The deduced amino acid sequence consists of 2224 amino acids including a 28 amino acid leader peptide. A direct comparison to human factor VIII reveals considerable homology between both proteins with respect to amino acid sequence and domain structure. A triplicated "A" domain and duplicated "C" domain show an approximate 40% identity to the corresponding domains in factor VIII. Factor V and Factor VIII both possess a heavily glycosylated B domain that separates the heavy and light chains of the activated cofactors, although no significant homology is observed in this region. The B domain of factor V contains 35 tandem and approximately 9 additional semi - conserved repeats of nine amino acids of the form (D-L-S-Q-T-T-L-S-P) and 2 additional semi-conserved repeats of 17 amino acids. Factor V contains 37 potential N-linked glycosylation sites, 25 of which are in the B domain, and a total of 19 cysteine residues. By direct comparison to amino acid sequence obtained from both human and bovine factor V, the thrombin (IIa) cleavage sites have been assigned as Arg-709/Ser-710, Arg-1018/Thr-1019, and Are-1545/Ser-1546. (Supported by NIH Grant HL-34575)
Microstructure and flocculation properties of hydrophobically associating acrylamide-modified cationic copolymer (2007)

The hydrophobically associating copolymers,poly(BS/AM/DMDAAC)(PBAD) were synthesized from acrylamide (AM),butyl styrene (BS) and dimethyldiallyammonium chloride (DMDAAC) by the micellar free radical copolymerization technique.The copolymers were characterized with UV and FT-IR.The atomic force microscope(AFM) measurement showed that aggregates had been formed in the aqueous solution of 0.005 g.dl -1 PBAD, and with the increase of PBAD concentration, the number and size of aggregates increased due to the intermolecular hydrophobic association of butylphenyl groups.The optimum flocculant concentration of PBAD was 0.005 g·dl -1 in the aqueous solution of 3% diatomite and the transmittance was 96.5%.The NaCl concentration obviously influenced the bridging and adsorption behavior of the copolymer for celite suspensions, and calcium and aluminium ions strengthened the flocculation effect of the polymer.
Conformal equivalence of triangle meshes (2008)

We present a new algorithm for conformal mesh parameterization. It is based on a precise notion of discrete conformal equivalence for triangle meshes which mimics the notion of conformal equivalence for smooth surfaces. The problem of finding a flat mesh that is discretely conformally equivalent to a given mesh can be solved efficiently by minimizing a convex energy function, whose Hessian turns out to be the well known cot-Laplace operator. This method can also be used to map a surface mesh to a parameter domain which is flat except for isolated cone singularities, and we show how these can be placed automatically in order to reduce the distortion of the parameterization. We present the salient features of the theory and elaborate the algorithms with a number of examples.
Penicillin-Resistant Streptococcus pneumoniae Strains Recovered in Houston: Identification and Molecular Characterization of Multiple Clones (1993)

A sample of 48 penicillin-resistant Streptococcus pneumoniae (PRSP) strains recovered between January 1989 and May 1991, primarily from infected children in Houston, was characterized by multilocus enzyme electrophoresis and repetitive extragenic palindromic-polymerase chain reaction genomic profiling. A heterogeneous array of 22 clonal genotypes was identified, but 64% of the PRSP strains in the sample were assigned to five clones that are closely similar in overall chromosomal character and express serotype 6 capsule. A close genetic association between these five clones and penicillin-resistant 6B clones recovered in Alaska, Iceland, and Spain was identified by multilocus enzyme electrophoresis. Taken together, the results suggest either that the common resistant 6B clones in Alaska, Iceland, Spain, and Houston have a recent ancestor or that isolates of a certain pneumococcal phylogenetic lineage are more likely to develop penicillin resistance.
Biosynthesis of tissue inhibitor of metalloproteinases by human fibroblasts in culture. Stimulation by 12-O-tetradecanoylphorbol 13-acetate and interleukin 1 in parallel with collagenase. (1985)

Biosynthesis of the glycoprotein tissue inhibitor of metalloproteinases (TIMP) by human fibroblasts in culture has been characterized by functional assays, immunoprecipitation, and immunocytochemistry with a monospecific antiserum. As determined by radiolabeling with [35S]methionine, immunoprecipitation, and analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the secreted form of TIMP had an Mr of 29,000, whereas the form associated with the cell layer had an Mr of 24,000. Unstimulated human lung fibroblasts (HFL-1) secreted TIMP at the rate of approximately 2 micrograms/10(6) cells/24 h, and normal foreskin fibroblasts (HS 27) and skin fibroblasts from a patient with Hurler's disease (GM 1391) secreted TIMP at 0.3 and 0.2 micrograms/10(6) cells/24 h, respectively. Secretion of TIMP was stimulated up to 10-fold by treating the cells with 20-100 ng/ml of 12-O-tetradecanoylphorbol 13-acetate or 10 units/ml of human interleukin 1. In the stimulated HFL-1 cells, TIMP accounted for 0.03-0.09% of the total [35S]methionine incorporated into protein, and 0.3-0.8% of the [35S]methionine in secreted protein. Although TIMP accounted for a relatively small proportion of total protein synthesis of the fibroblasts, greater than 80% of untreated and greater than 95% of stimulated fibroblasts synthesized TIMP, as determined by indirect immunofluorescence. The treatments of the human fibroblasts that increased TIMP secretion also induced synthesis and secretion of proenzyme forms of collagenase, indicating that degradative enzymes and their controlling inhibitors may be synthesized in parallel under certain conditions.
Tobacco smoke particulate matter chemistry by NMR (2006)

The submicron liquid droplets constituting the particulate matter of mainstream tobacco smoke (PMMTS) are viscous and of a composition that is complex and poorly understood. PMMTS is often approximately 80% w/w 'tar' where 'tar'=total PMMTS- (nicotine+water). Many of the chemical agents in MTS responsible for smoking-related cancers are found at least partially in the PMMTS portion of MTS. The properties of PMMTS vary with brand and with puffing patterns. The chemical forms and total levels of nicotine, the identities/levels of other compositionally dominant compounds, and the identities/levels of carcinogens are of interest. Most studies of the composition of PMMTS have involved extraction then chromatography. Such methods allow the determination of low-level constituents, but alter the samples such that direct information regarding chemical conditions within the PMMTS cannot be obtained. Here, we utilize nuclear magnetic resonance spectroscopy (NMR) to examine native PMMTS in conventional cigarettes, including measurements of the brand-dependent fraction of PMMTS nicotine that is in the free-base form (increasing this fraction in inhaled tobacco smoke affects the rates of the processes governing nicotine deposition in the respiratory tract, and so has implications for smoking behavior and addiction). We also demonstrate the use of NMR for characterizing the composition of PMMTS (including the levels of selected cigarette additives) when the cosolvent DMSO-d6 is added to improve spectral resolution. The native and solvent-assisted results open the door to a range of future studies.
Honey Amylase Activity and Food Starch Degradation (2002)

ABSTRACT: Honey amylase was evaluated for potential to degrade food starch and cause viscosity loss. Honey was assayed for amylase activity with diastase number (DN). A viscosity assay, developed with unmodified waxy maize starch, measured honey amylase rate of viscosity decrease (RVD). The relationship between DN and RVD was linear (R 2 = 0.98). Modified waxy maize starches showed resistance to honey amylase. Honey heat treatment at 85 °C reduced amylase activity 2 to 5 DN, but confirmed enzyme heat resistance. Optimum pH for honey amylase was confirmed at pH 5.3 to 5.6. RVD activity declined as pH decreased. Preventing food viscosity loss involved selecting honey with lowered DN and/or using modified starches. Complete control of activity was achieved in barbecue sauce at pH < 3.9.
Method for Applying Interlink Correlation to Multilink MIMO Propagation Channel Estimation (2011)

This paper proposes a method for applying interlink correlation to evaluate a multiuser-multiple input multiple output (MU-MIMO) system and describes measurement of a multi-link MIMO propagation channel. The proposed method is validated through comparison of measurement results to those of the conventional MIMO propagation channel model. The results show that the proposed method employing the Weichselberger model can handle the interlink correlation property, and accurately reconstructs a MIMO propagation channel.
Water-solubilisation and bio-conjugation of a red-emitting BODIPY marker (2010)

The synthesis and preliminary bio-conjugation studies of a novel water-soluble red-emitting di-styryl BODIPY dye are disclosed. Aggregation behaviour of this compound under physiological conditions was suppressed by specific introduction of a di-sulfonated peptide-based linker at the meso phenyl substituent, sultonated styryl arms and short polyethyleneglycol chains at the boron center. Thus, a good quantum yield of 22% in PBS for this red-emitting BODIPY was obtained. Introduction of an activated ester function enabled successful bio-conjugation to monoclonal antibodies and proteins.
GMPPT of solar PV array under partial shading condition using LabVIEW FPGA (2015)

The performance of a solar photovoltaic (PV) system mainly depends upon the solar radiation level and temperature. The total power generated by the PV array is affected by partial shading conditions and it gets decreased upon shading. In order to extract maximum power under partial shading condition this paper proposes a two-step, maxima of all peak power points (MPPP) algorithm for global maximum power point tracking (GMPPT) using LabVIEW FPGA module. In the proposed algorithm the first step is to determine the value of solar PV array voltage at the global maxima of the P-V characteristics. The second step is a duty cycle control using perturb & observe (P&O) method. The proposed algorithm is executed to track the GMPP using boost DC-DC converter. The partial shading condition is detected by power threshold logic. The performance of the proposed method is evaluated through Power Simulation Computer Aided Design (PSCAD) simulation software. For real time implementation a solar array simulator (SAS) E4260A, is used for laboratory generation of solar power. The GMPPT algorithm for SAS and duty ratio control for boost DC-DC converter is implemented on FPGA controller using LabVIEW platform.
Thermal Effects in Kilowatt Fiber Lasers (2004)

Thermal effects and their influences on kilowatt ytterbium-doped double-clad (YDDC) fiber lasers are studied through numerical modeling. Solutions to suppress the thermal effects in the YDDC fiber lasers under bidirectional end pump and distributed pump are presented and compared for the first time. It is shown that lower operating temperature and more uniform heat dissipation in fibers can be obtained by optimizing the arrangement of pump powers, pump absorption coefficients, and fiber lengths.
Infant Brain Atlases from Neonates to 1- and 2-Year-Olds (2011)

We expect that the proposed infant 0-1-2 brain atlases would be significantly conducive to structural and functional studies of the infant brains. These atlases are publicly available in our website, http://bric.unc.edu/ideagroup/free-softwares/.
Liquid crystal alignment on surfactant treated obliquely evaporated surfaces (1980)

We report observations on the alignment of nematic liquid crystals on surfaces of obliquely deposited silicon monoxide, with and without additional treatment with surfactant. The liquid crystals reported on are 4.4-dihexylazoxybenzene and 4-heptyl-4’-cyanobipheny. The surfactants used are N,N-dimethyl-N-octadecyl-3-aminopropyltrimethoxysilyl chloride and plasma polymerized tetrafluoroethylene. While untreated silicon monoxide surfaces produce inclined alignment, with the liquid crystal director oriented in the same quadrant as the direction of deposition, it was found that treatment with surfactant can result in an inclined alignment away from the direction of deposition, with the director now oriented nearly perpendicular to the direction obtained in the absence of surfactant. A simple model to explain these observations is presented.
Shaping Early Reorganization of Neural Networks Promotes Motor Function after Stroke (2016)

Neural plasticity is a major factor driving cortical reorganization after stroke. We here tested whether repetitively enhancing motor cortex plasticity by means of intermittent theta-burst stimulation (iTBS) prior to physiotherapy might promote recovery of function early after stroke. Functional magnetic resonance imaging (fMRI) was used to elucidate underlying neural mechanisms. Twenty-six hospitalized, first-ever stroke patients (time since stroke: 1-16 days) with hand motor deficits were enrolled in a sham-controlled design and pseudo-randomized into 2 groups. iTBS was administered prior to physiotherapy on 5 consecutive days either over ipsilesional primary motor cortex (M1-stimulation group) or parieto-occipital vertex (control-stimulation group). Hand motor function, cortical excitability, and resting-state fMRI were assessed 1 day prior to the first stimulation and 1 day after the last stimulation. Recovery of grip strength was significantly stronger in the M1-stimulation compared to the control-stimulation group. Higher levels of motor network connectivity were associated with better motor outcome. Consistently, control-stimulated patients featured a decrease in intra- and interhemispheric connectivity of the motor network, which was absent in the M1-stimulation group. Hence, adding iTBS to prime physiotherapy in recovering stroke patients seems to interfere with motor network degradation, possibly reflecting alleviation of post-stroke diaschisis.