gebhart/patrepeval huggingface dataset:
Albumin modulates lateral assembly of fibrin polymers: evidence of enhanced fine fibril formation and of unique synergism with fibrinogen (1987)

We identified a new property of human albumin. It enhances formation of fine fibril (or leptofibril) structures during fibrin gelation, and by nephelometric and electron microscopic measurements, this property is independent of and synergistic with that of fibrinogen. We examined fibrin aggregation using physiologic temperatures and pH and albumin:fibrin concentration ratios below those at which the known accelerating effect on fibrin aggregation occurs. An albumin concentration dependent decrease in gel turbidity maxima was consistently demonstrable in buffers containing or lacking (2-5 mM) CaCl2. This decrease was shown to be induced by albumin preparations which had been exposed to 2 mM ethylene-diaminetetraacetate disodium salt (EDTA), dialyzed, and tested in EDTA-free buffer. A delay in the onset of aggregation was also shown in calcium-lacking buffers by use of either reaggregating fibrin or fibrinogen aggregated with low (0.01-0.05 unit/mL) thrombin concentrations. Rates of fibrin aggregation as well as those of fibrinopeptide release were not affected by albumin, and the decrease in gel absorbance was demonstrable when solubilized fibrin was reaggregated at all final fibrin concentrations (0.2-4 microM) examined. Computed from wavelength dependence turbidity measurements (1 microM fibrin, I = 0.20), albumin decreased the average mass:length ratio from 8.24 X 10(11) to 4.26 X 10(11) daltons/cm, or from that of an approximately six to a three protofibril-thick strand. It also decreased the mean fibril radius from 48.5 to 36.4 nm but had no effect on fibril density. Electron microscopic measurements of cross-sectional fibril widths, performed on sections of glutaraldehyde-fixed gels, disclosed differences between albumin-containing and control gels which were significant by chi 2 analysis (P greater than 0.001). Fibril groups of 7-20- and 21-40-nm width together comprised 77% of fibrils formed in the presence of albumin (n = 251) compared to 30% of controls (n = 309). Conversely, coarser fibrils of 41-60- and 61-97-nm width together comprised 23% of fibrils formed in the presence of albumin and 70% of controls. This albumin effect was demonstrable by use of different monomeric albumin preparations including defatted, undefatted (unexposed and exposed to 60 degrees C, 10 h), chromatographically [gel exclusion and (diethylaminoethyl)cellulose] pure, S-(carboxymethyl)albumin, and S-(N-ethylsuccinimidyl)albumin. Chromatographically isolated albumin oligomers lacked this property, suggesting that a specific site(s) on albumin was (were) required.(ABSTRACT TRUNCATED AT 400 WORDS)
Functional demonstrations of starch binding domains present in Ostreococcus tauri starch synthases isoforms (2015)

The results presented here suggest that differences between OsttaSSIII-A and SSIII-B SBDs in the number of and binding of amino acid residues may produce differential affinities for each isoform to polysaccharides. Increasing the knowledge about SBDs may lead to their employment in biomedical and industrial applications.
The Next Generation of Advanced Spectroscopy: Surface Enhanced Raman Scattering from Metal Nanoparticles (2010)

Shining light: Surface enhanced Raman scattering (SERS) offers much in terms of molecularly specific information at ultrasensitive levels. Two recent breakthroughs have occured in this field. First, a new surface was developed that provides SERS information previously inaccessible, and second, a nanoparticle SERS label was introduced to image antigens relating to cancer in tissue.
A family of modular type mannuronan C‐5‐epimerase genes controls alginate structure in <i>Azotobacter vinelandii</i> (1995)

The L-guluronic acid residues in the Azotobacter vinelandii polysaccharide alginate originate from a post-polymerization reaction catalysed by the enzyme mannuronan C-5-epimerase (ME). We have previously reported the cloning and expression of an A. vinelandii gene encoding this enzyme, and we show here that the organism encodes at least four other ME genes originating from a common ancestor gene by a complex rearrangement process. The biological function of the corresponding enzymes is probably to catalyse the formation of alginates with a variety of physical properties. This model may explain the origin of the structural variability found in alginates isolated both from prokaryotic and eukaryotic organisms. The A. vinelandii enzymes may also potentially be useful for certain medical and biotechnological applications of this commercially important polysaccharide.
Ultrafast Intersystem Crossing and Spin Dynamics of Photoexcited Perylene-3,4:9,10-bis(dicarboximide) Covalently Linked to a Nitroxide Radical at Fixed Distances (2009)

Time-resolved transient optical absorption and EPR (TREPR) spectroscopies are used to probe the interaction of the lowest excited singlet state of perylene-3,4:9,10-bis(dicarboximide) ((1*)PDI) with a stable tert-butylphenylnitroxide radical ((2)BPNO(*)) at specific distances and orientations. The (2)BPNO(*) radical is connected to the PDI with the nitroxide and imide nitrogen atoms either para (1) or meta (3) to one another, as well as through a second intervening p-phenylene spacer (2). Transient absorption experiments on 1-3 reveal that (1*)PDI undergoes ultrafast enhanced intersystem crossing and internal conversion with tau approximately = 2 ps to give structurally dependent 8-31% yields of (3*)PDI. Energy- and electron-transfer quenching of (1*)PDI by (2)BPNO(*) are excluded on energetic and spectroscopic grounds. TREPR experiments at high magnetic fields (3.4 T, 94 GHz) show that the photogenerated three-spin system consists of the strongly coupled unpaired electrons confined to (3*)PDI, which are each weakly coupled to the unpaired electron on (2)BPNO(*) to form excited doublet (D(1)) and quartet (Q) states, which are both spectrally resolved from the (2)BPNO(*) (D(0)) ground state. The initial spin polarizations of D(1) and Q are emissive for 1 and 2 and absorptive for 3, which evolve over time to the opposite spin polarization. The subsequent decays of D(1) and Q to ground-state spin polarize D(0). The rates of polarization transfer depend on the molecular connectivity between PDI and (2)BPNO(*) and can be rationalized in terms of the dependence on molecular structure of the through-bond electronic coupling between these species.
Ship movement anomaly detection using specialized distance measures (2015)

This paper provides a solution for anomaly detection in maritime traffic domain based on the clustering results presented in a previous work. That work created clusters for vessels moving close to shores by associating vessel movements with International Maritime Organization Rules (especially Traffic Separation Scheme Boundaries). In this paper, we show how three division distances with the clusters can detect anomalous navigational behaviors. The proposed method decides for each trajectory point if the vessel is anomalous, considering longitude, latitude, speed and direction. Although the approach is point-based, which is applicable for real-time AIS surveillance, it is also flexible enough for analysts to set their own threshold for labeling whole trajectories.
Preclinical Efficacy and Safety Assessment of an Antibody–Drug Conjugate Targeting the c-RET Proto-Oncogene for Breast Carcinoma (2015)

RET is broadly expressed in breast cancer specimens and thus represents a potential therapeutic target; Y078-DM1 and Y078-DM4 demonstrated antitumor activity in preclinical models. Optimization of the dosing schedule or an alternate cytotoxic agent with a different mechanism of action may reduce the potential risk of neuropathy. Clin Cancer Res; 21(24); 5552-62. ©2015 AACR.
Étude analytique et critique de l'ouvrage : Les races humaines du Soudan Français de M. Sarrazin (1902)

Anthony R., Huguet J. Étude analytique et critique de l'ouvrage : Les races humaines du Soudan Français de M. Sarrazin. In: Bulletins de la Société d'anthropologie de Paris, V° Série. Tome 3, 1902. pp. 842-849.
Pulmonary Hypertension in Pulmonary Langerhans Cell Granulomatosis (2012)

Introduction. Pulmonary Langerhans cell granulomatosis is a rare disease with a variable course. In pulmonary Langerhans cell granulomatosis pulmonary hypertension is frequent and has an independent prognostic impact. A vasculopathy which ist not related to ventilatory disturbance and fibrosis has been identified. An arteriopathy and even a venulopathy have been described. Due to this possible venulopathy vasodilators carry a significant risk for pulmonary congestion and edema. No drugs have been approved until now. Case Presentation. One female with PLCG developed severe PH four years after primary diagnosis of pulmonary Langerhans cell granulomatosis. Retrospective analysis of lung biopsies revealed an arterial vasculopathy at the time of primary diagnosis without clinical signs of PH at this time. Sildenafil led to a sustained improvement of hemodynamic features and exercise capacity. Conclusion. This paper underlines that patients with PLCG with an arterial vasculopathy-related PH might improve under sildenafil. Further trials addressing treatment of PH and vasculopathy are needed.
Islet   cell failure in type 2 diabetes (2006)

The major focus of this Review is on the mechanisms of islet beta cell failure in the pathogenesis of obesity-associated type 2 diabetes (T2D). As this demise occurs within the context of beta cell compensation for insulin resistance, consideration is also given to the mechanisms involved in the compensation process, including mechanisms for expansion of beta cell mass and for enhanced beta cell performance. The importance of genetic, intrauterine, and environmental factors in the determination of "susceptible" islets and overall risk for T2D is reviewed. The likely mechanisms of beta cell failure are discussed within the two broad categories: those with initiation and those with progression roles.
Influence of Pancreatic Islets on Growth and Differentiation of Hepatocytes in Co-Culture (1999)

Improvement of cell culture conditions in hepatic tissue engineering may permit cell/tissue banking and the generation of liver tissue equivalents for transplantation. In these systems, continuous hepatotrophic stimulation is still necessary. We investigated the stimulatory effects of pancreatic islets on hepatocytes in co-culture and characterized the stimulatory mechanisms. Hepatocytes and pancreatic islets were harvested from Lewis rats. Cells were cultured on collagen dishes either with nonstimulated media (controls and co-cultures with low or high islet rate) or stimulated media (controls and co-cultures). To characterize stimulatory mechanisms, additional co-cultures with membrane separation, with antiinsulin, antiglucagon, and with both antibodies were examined. Hepatocyte numbers, albumin secretion rate by enzyme-linked immunoadsorbent assay, and monoethylglycinxylidid biotransformation values by fluorescence polarization immunoassay were assessed. A radioimmunoassay measured insulin and glucagon concentrations. In groups with nonstimulated media, cell number was higher in co-cultures with low islet rate, and albumin secretion rate was increased in co-cultures with high islet rate compared to controls. MEGX biotransformation was decreased in co-cultures. In groups with stimulated media, co-culture had no impact on cell number or albumin secretion rate. Hepatocyte numbers and albumin secretion rates were not changed in co-cultures after membrane separation. Islet effects on hepatocytes were reduced in co-cultures with antiinsulin, antiglucagon, or both antibodies. Pancreatic islets provide stimulation for hepatocytes in vitro. Islet effects were mediated by soluble factors, and are dependent on insulin and glucagon. These results permit further investigations towards three-dimensional transplantable hepatocyte-islet devices for continuous in vitro and in vivo stimulation.
Reproducible Dendronized PEG Hydrogels via SPAAC Cross-Linking (2017)

A common issue with hydrogel formulations is batch-to-batch irreproducibility originating from poorly defined polymer precursors. Here, we report the use of dendritic polymer end-groups to address this issue and maintain reproducibility between batches of poly(ethylene glycol) (PEG) hydrogels. Specifically, we synthesized two end-functionalized PEG chains: one with azide-terminated first- and second-generation dendrons and the other with strained cyclooctynes. The two complementary azide and alkyne polymers react via strain-promoted alkyne-azide cycloaddition (SPAAC) to produce hydrogels quickly in the absence of additional reagents or catalyst at low polymer concentrations. Hydrogels made with first-generation dendrons gelled in minutes and exhibited a small degree of swelling when incubated in PBS buffer at 37 °C, whereas hydrogels made from second-generation dendrons gelled in seconds with almost no swelling upon incubation at 37 °C. In both cases, the hydrogels proved reproducible, resulting in identical Young's modulus values from different batches. The hydrogels prepared with second-generation dendrons were seeded with human mesenchymal stem cells and showed high cell viability as well as cell spreading over a two-week time frame. These studies show that the SPAAC hydrogels are noncytotoxic and are capable of supporting cell growth.
Compressive Residual Strains in Mineral Nanoparticles as a Possible Origin of Enhanced Crack Resistance in Human Tooth Dentin (2015)

The tough bulk of dentin in teeth supports enamel, creating cutting and grinding biostructures with superior failure resistance that is not fully understood. Synchrotron-based diffraction methods, utilizing micro- and nanofocused X-ray beams, reveal that the nm-sized mineral particles aligned with collagen are precompressed and that the residual strains vanish upon mild annealing. We show the link between the mineral nanoparticles and known damage propagation trajectories in dentin, suggesting a previously overlooked compression-mediated toughening mechanism.
Novel synthesis of .alpha.-substituted acrylic acids (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel synthesis of .alpha.-substituted acrylic acidsS. Serota, J. R. Simon, E. B. Murray, and W. M. LinfieldCite this: J. Org. Chem. 1981, 46, 21, 4147–4151Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/jo00334a008https://doi.org/10.1021/jo00334a008research-articleACS PublicationsRequest reuse permissionsArticle Views569Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Impact of strengthening fluids on roughness of 3D printed models (2015)

For some applications, 3D printed parts usually do not have satisfactory mechanical properties, so to broaden their usage, additive technologies should be combined with the well-known metallurgical processes, such as investment and others casting techniques.3D printing developers persistently introduce new base materials and strengthening fluids which may cause different surface roughness.Therefore, in this paper, the authors have tested the roughness of 3D printed samples strengthened with common, but also with alternative fluids.Measurements proved that fluids do have significant influence on the roughness.
Improvements to Digital Carrier Phase Recovery Algorithm for High-Performance Optical Coherent Receivers (2010)

The Viterbi-and-Viterbi (V-V) algorithm is widely used to recover the carrier phase in optical digital coherent receivers. For simplicity, the basic V-V algorithm assumes constant carrier phase within the average duration. However, this basic assumption is probably violated by factors such as laser frequency offset and nonlinear XPM. In order to improve the basic V-V carrier phase recovery, five methods are introduced, verified, and analyzed. All these methods are compatible with parallel implementation that is mandatory for a realistic DSP circuit. The Q-improvement brought by each algorithm is analyzed together with the complexity of each. Among the five methods, the laser frequency offset compensation expands the tolerable frequency offset to 0.37 symbol rate, and the optimum weighted averaging in conjunction with normalization processing improves the Q-value by 2 dB under severe XPM condition.
Using types to analyze and optimize object-oriented programs (2001)

Object-oriented programming languages provide many software engineering benefits, but these often come at a performance cost. Object-oriented programs make extensive use of method invocations and pointer dereferences, both of which are potentially costly on modern machines. We show how to use types to produce effective, yet simple, techniques that reduce the costs of these features in Modula-3, a statically typed, object-oriented language. Our compiler performs type-based alias analysis to disambiguate memory references. It uses the results of the type-based alias analysis to eliminate redundant memory references and to replace monomorphic method invocation sites with direct calls. Using limit, static, and running time evaluation, we demonstrate that these techniques are effective, and sometimes perfect for a set of Modula-3 benchmarks.
CDMA unslotted ALOHA systems with finite buffers (2002)

A CDMA unslotted ALOHA system with finite size of queueing buffers is discussed. We introduce an analytical model in which the system is divided into two Markov chains; one is in user part, and the other is in channel part. We analyze the system performance using this analytical model, and evaluate the effect of buffer capacity in terms of the throughput, rejection probability and the average delay.
RETRACTED: Purification and Identification of p68 RNA Helicase Acting as a Transcriptional Coactivator Specific for the Activation Function 1 of Human Estrogen Receptor α (1999)

The estrogen receptor (ER) regulates the expression of target genes in a ligand-dependent manner. The ligand-dependent activation function AF-2 of the ER is located in the ligand binding domain (LBD), while the N-terminal A/B domain (AF-1) functions in a ligand-independent manner when isolated from the LBD. AF-1 and AF-2 exhibit cell type and promoter context specificity. Furthermore, the AF-1 activity of the human ERalpha (hERalpha) is enhanced through phosphorylation of the Ser(118) residue by mitogen-activated protein kinase (MAPK). From MCF-7 cells, we purified and cloned a 68-kDa protein (p68) which interacted with the A/B domain but not with the LBD of hERalpha. Phosphorylation of hERalpha Ser(118) potentiated the interaction with p68. We demonstrate that p68 enhanced the activity of AF-1 but not AF-2 and the estrogen-induced as well as the anti-estrogen-induced transcriptional activity of the full-length ERalpha in a cell-type-specific manner. However, it did not potentiate AF-1 or AF-2 of ERbeta, androgen receptor, retinoic acid receptor alpha, or mineralocorticoid receptor. We also show that the RNA helicase activity previously ascribed to p68 is dispensable for the ERalpha AF-1 coactivator activity and that p68 binds to CBP in vitro. Furthermore, the interaction region for p68 in the ERalpha A/B domain was essential for the full activity of hERalpha AF-1. Taken together, these findings show that p68 acts as a coactivator specific for the ERalpha AF-1 and strongly suggest that the interaction between p68 and the hERalpha A/B domain is regulated by MAPK-induced phosphorylation of Ser(118).
Tratamiento quirúrgico de la atrofia congenita del prepucio posterior en el perro (1986)

Las anomalias congenitas del prepucio en el perro que con mas incidencia se presentan en la Clinica Canina son la fimosis y la parafimosis. Sin embargo, la ausencia congenita de la vaina prepucial, tanto completa como incompleta, en las que el pene se encuentra mas o menos desprotegida y por lo tanto sometido a posibles traumatismos y desecacion ambiental son muy infrecuentes, siendo muy reducida tambien la bibliografia existente al respecto.
En el presente trabajo, y sobre la iconografia aportada, se describe un caso de atrofia 
congenita del segmento caudal del prepucio en un perro Mastin Leones de tres meses de edad, para el cual hemos puesto a punto una intervencion quirurgica plastica correctora, de caracter original, con objeto de su tratamiento.
Synthesis and properties of surfactants based on carbohydrates I. Synthesis of some O-dodecyl aldoses (1978)

Article Synthesis and properties of surfactants based on carbohydrates I. Synthesis of some O-dodecyl aldoses was published on March 1, 1978 in the journal Tenside Surfactants Detergents (volume 15, issue 2).
Multiple Effects of Leucine on Glue agon, Insulin, and Somatostatin Secretion from the Perfused Rat Pancreas* (1985)

The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucose concentration (3.3 mmol/liter), leucine caused a dose-related biphasic increase in glucagon output in the absence of arginine, but only a transient increase in the presence of the latter amino acid. These positive responses were markedly reduced and, on occasion, abolished at a high glucose concentration (8.3 mmol/liter). Moreover, at a low glucose concentration (3.3 mmol/liter) and in the presence of arginine, the highest concentration of leucine (15.0 mmol/liter) provoked a sustained and reversible inhibition of glucagon release. Likewise, leucine (15.0 mmol/liter) reversibly inhibited glucagon secretion evoked by glutamine in the absence of glucose. Thus, leucine exerted a dual effect on the secretion of glucagon, the inhibitory effect of leucine prevailing at a high concentration of the branched chain amino acid and when glucagon secretion was already stimulated by arginine or glutamine. At a physiological concentration (0.2 mmol/liter), however, leucine was a positive stimulus for glucagon release, especially in the absence of another amino acid. Concomitantly, leucine was always a positive stimulus for both insulin and somatostatin secretion. The intimate mechanisms involved in the dual effect of leucine on glucagon secretion remain to be elucidated.