gebhart/patrepeval huggingface dataset:
Plant pharming of a full‐sized, tumour‐targeting antibody using different expression strategies (2008)

The aims of this work were to obtain a human antibody against the tumour-associated antigen tenascin-C (TNC) and to compare the yield and quality of plant-produced antibody in either stable transgenics or using a transient expression system. To this end, the characterization of a full-sized human immunoglobulin G (IgG) [monoclonal antibody H10 (mAb H10)], derived from a selected single-chain variable fragment (scFv) and produced in plants, is presented. The human mAb gene was engineered for plant expression, and Nicotiana tabacum transgenic lines expressing both heavy (HC) and light (LC) chain were obtained and evaluated for antibody expression levels, in vivo assembly and functionality. Affinity-purified H10 from transgenics (yield, 0.6-1.1 mg/kg fresh weight) revealed that more than 90% of HC was specifically degraded, leading to the formation of functional antigen-binding fragments (Fab). Consequently, H10 was transiently expressed in Nicotiana benthamiana plants through an Agrobacterium-mediated gene-transfer system. Moreover, the use of the p19 silencing suppressor gene from artichoke mottled crinkle virus raised antibody expression levels by an order of magnitude (yields of purified H10, 50-100 mg/kg fresh weight). Approximately 75% of purified protein consisted of full-sized antibody functionally binding to TNC (K(D) = 14 nm), and immunohistochemical analysis on tumour tissues revealed specific accumulation around tumour blood vessels. The data indicate that the purification yields of mAb H10, using a transient expression system boosted by the p19 silencing suppressor, are exceptionally high when compared with the results reported previously, providing a technique for the over-expression of anticancer mAbs by a rapid, cost-effective, molecular farming approach.
Contribution to the determination of vanishing points using Hough transform (1994)

We propose a method to locate three vanishing points on an image, corresponding to three orthogonal directions of the scene. This method is based on two cascaded Hough transforms. We show that, even in the case of synthetic images of high quality, a naive approach may fail, essentially because of the errors due to the limitation of the image size. We take into account these errors as well as errors due to detection inaccuracy of the image segments, and provide a method efficient, even in the case of real complex scenes.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The anti-lymphoma effect of antibody-mediated immunotherapy is based on an increased degranulation of peripheral blood natural killer (NK) cells (2006)

We were able to quantify and characterize NK cells mediating ADCC with a new and feasible method. The CD107a assay may be useful for predicting treatment responses of individual patients and may help find the optimal dosage and timing for treatment with mAb.
Genetic control of inflammatory gene induction and NF-kappa B-like transcription factor activation in response to an atherogenic diet in mice. (1993)

A high fat, high cholesterol "atherogenic" diet induced considerably greater hepatic levels of conjugated dienes and expression of several inflammatory and oxidative stress responsive genes (JE, the mouse homologue of monocyte chemotactic protein-1, colony-stimulating factors, heme oxygenase, and members of the serum amyloid A family) in fatty streak susceptible C57BL/6 mice compared to fatty streak resistant C3H/HeJ mice. Since serum amyloid A proteins bind exclusively to HDL and influence the properties of HDL, serum amyloid A expression may contribute to the decrease in HDL levels seen in the susceptible strains. Induction of a similar set of genes was observed upon injection of minimally oxidized low density lipoprotein. The transcription factor NF-kappa B is known to be activated by oxidative stress and is involved in the transcriptional regulation of several of these genes. On the atherogenic diet the susceptible C57BL/6 mice exhibited significant NF-kappa B-like activation whereas the resistant C3H/HeJ mice exhibited little or no activation. These results are consistent with the hypothesis that the atherogenic diet resulted in the accumulation of oxidized lipids in certain tissues (e.g., liver and arteries) and the resulting inflammatory response to this oxidative stress was genetically determined.
Automatic human brain vessel segmentation from 3D 7 Tesla MRA images using fast marching with anisotropic directional prior (2012)

Accurate 3D models of the human brain vessels can greatly help to diagnose serious diseases. Such models can be constructed by segmentation of 3D MRA images, especially the recently introduced high resolution 7T MRA. We propose a new two-step approach for fully automatic segmentation of 7T MRA images of the human cerebrovascular system. First, a 3D model-based approach is applied to segment thick vessels and most parts of thin vessels. Then, the missing vessel parts, which are caused by low contrast and high noise, are completed using a novel fast marching approach with anisotropic directional prior. An evaluation of our approach and a comparison with two previous approaches have been conducted using high resolution 3D 7T MRA images.
Glucose uptake and glycogen synthesis in normal and chronically active muscles (1993)

The hindlimb muscles of the C57Bl6J dy2J/dy2J (dy2J) mouse suffer from a chronic neural stimulation (pseudomyotonia), resulting in increased contractile activity. In response to the increased contractile activity, these muscles store increased amounts of glycogen. In this study, glucose uptake and glycogenesis (glycogen synthesis from glucose) were analyzed in chronically active and normal muscles. In vivo experiments demonstrate increased 3-O-methylglucose (3-MG) uptake rates and glycogenesis by chronically active dy2J gastrocnemius muscles (Gast) vs. normal control Gast. The chronically active diaphragm muscle (Dia) showed the highest rates of 3-MG uptake, as well as glycogenesis in vivo when compared with other skeletal muscles. No differences were observed between dy2J and control Dia. The levels of blood glucose were similar between dy2J and control animals. In vitro experiments demonstrated an increased sensitivity and responsiveness to insulin for glucose uptake in the dy2J soleus muscle (Sol). Glycogenesis by dy2J Sol was elevated only at the highest insulin concentration tested (10,000 microU/ml). In contrast, the dy2J extensor digitorum longus muscle had an increased sensitivity and responsiveness to insulin for both glucose uptake and glycogenesis. This study demonstrates that chronically active muscles have elevated capacities for glucose uptake and glycogenesis and may help to explain the elevated glycogen levels in the dy2J hindlimb muscles.
Next generation pixel scaling for the digital micromirror device (2005)

Digital light processing (DLP/spl trade/) technology, is the leader in the microdisplay market using the digital micromirror device (DMD) technology. As the market for display resolution and performance grows, our strategy is focusing on decreasing the pixel pitch for the latest high resolution devices. This paper presents the issues that are associated with shrinking the micromirror and actuation circuitry, as well as design and process techniques that are used to overcome the scaling requirements. Simulation results illustrate the ability of the next generation DMD to meet these scaling requirements to overcome the electrostatic, mechanical, and optical constraints at the smaller pixel pitch.
Characterization of chromosome 1 abnormalities in malignant melanomas (2000)

Chromosome 1 abnormalities are the most commonly detected aberrations in many cancers including malignant melanomas. Specific breakpoints are reported for malignant melanomas throughout the chromosome but especially at 1p36 and at several sites throughout 1p22-q21. In addition, partial deletions and loss of heterozygosity have been found on 1p indicating the possible location of tumor suppressor genes. Here we have characterized the involvement of chromosome 1 in a series of seven malignant melanoma cell lines. Initial chromosome painting studies revealed that six of the cell lines had chromosome 1 rearrangements. Deletions involving 1p10-32, 1q11-44, and 1q25-44 were observed. The other rearrangement breakpoints included three in the 1q10-p11 region with the rest at 1p36, 1p34, 1p32, 1p31, 1p12-13, 1q21, and 1q23. The breaks at 1q10-p11 were investigated further using an alpha-satellite 1 centromere probe and yeast artificial chromosomes (YACs) from the region. Two of the 1q10-p11 breaks mapped in the centromeric region, while the others mapped to variable sites. This suggests that the role of these rearrangements in the pathogenesis of melanomas does not involve the alteration of specific oncogenes in the breakpoint region. During the YAC mapping a previously undetected, small (<1 Mbp) del(1)(p10p11) was identified. This deletion lies within minimal overlapping deleted regions reported in head and neck as well as breast carcinomas and it could therefore facilitate the isolation of a carcinoma-associated tumor suppressor gene.
Synthesis and Characterization of Calcium Silicate from Rice Husk Ash and Shell of Snail <i>Pomacea canaliculata</i> by Solid State Reaction (2015)

Calcium silicate was successfully synthesized using agricultural byproduct such as rice husk ash and shell of snail namely Pomacea canaliculata by solid state reaction. Chemical composition of silica in rice husk ash and calcium oxide in shell were 91.50 and 98.25%, respectively by X-ray fluorescent determination. The rice husk ash and shell in molar ratio of 1:1 were milled for 5-8 hr and calcined at 800-1000 ° C for 2 hr. The Fourier transforms infrared spectra and X-ray diffractometry pattern were confirmed the formation of calcium silicate as the milling time increased.
Synthesis via vinyl sulfones. 21. Total synthesis of dl-morphine (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis via vinyl sulfones. 21. Total synthesis of dl-morphineJ. E. Toth and P. L. FuchsCite this: J. Org. Chem. 1987, 52, 3, 473–475Publication Date (Print):February 1, 1987Publication History Published online1 May 2002Published inissue 1 February 1987https://pubs.acs.org/doi/10.1021/jo00379a038https://doi.org/10.1021/jo00379a038research-articleACS PublicationsRequest reuse permissionsArticle Views1604Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Nonredundant successive approximation register forA/D converters (1996)

A successive approximation register for N bit A/D converters is presented. It codes the possible 2N conversion output values with the minimum number of FF (log2N), as it is nonredundant and very simple. It allows area optimisation and minor code probability error.
Full vector wave calculation of photonic band structures in face-centered-cubic dielectric media (1990)

The photonic band structure of a face-centered-cubic lattice of spheres is calculated using the plane-wave method for Maxwell's equations. Comparisons with the available experimental results show rather good agreement, except that we do not find a true gap for this configuration. This we believe is due to symmetry reasons at the W point.
Comparison of Power Densities and Chemical Potential Variation in Solid Oxide Fuel Cells with Multilayer and Single‐Layer Oxide Electrolytes (1998)

Several multilayer and single‐layer mixed conducting oxide structures are compared for their use as electrolytes in solid oxide fuel cells. Detailed calculations of the power density characteristics and the variation of the oxygen chemical potential gradient as a function of the external load and thickness of the oxide layers are provided. Engineering implications of the analysis in terms of designing efficient as well as mechanically and chemically stable fuel cells with a layered electrolyte structure are also provided.
Study of depression and anxiety in patients with type-2 diabetes mellitus (2012)

OBJECTIVE To explore the emotion and its effective factors in patients with type-2 diabetes mellitus,which will provide clinical,experimental and theoretical bases on practicing new treating methods.METHODS 80 type-2 diabetes mellitus cases which meeting criteria of World Health Organization(1999)for diabetes,including 40 complicated cases and 40 no-complicated cases;40 healthy people were selected as the healthy control group.All the subjective were tested by the following questionnaires:general condition questionnaires,EPQ,SCSQ,LES.However,HAMD and HAMA were rated by the researcher.RESULTS The type-2 diabetes mellitus cases had obviously depression and anxious,the total scores of HAMD and HAMA in the complicated group were higher than that in the no-complicated group(P﹤0.01);The total scores of LES,especially the negative life events scale,in type-2 diabetes mellitus group were higher than that in the healthy person group(P﹤0.01);The total scores of the positive coping style in the complicated group were lower than that in the no-complicated group and the healthy person group(P﹤0.01);The complicated group had higher EPQ-N、EPQ-L scores than the no-complicated group and the healthy people group(P﹤0.01,P﹤0.05);The type-2 diabetes mellitus group had lower SES scores than the healthy person group,and the complicated group was the lowest(P﹤0.01);The correlative analysis showed significant positive correlation between the EPQ-N,the negative life events scale and HAMD and HAMA(P﹤0.01);But there were significant negative correlations between the positive coping style,SES scores and HAMD and HAMA(P﹤0.01).CONCLUSION The ratio of depression and anxiety in type-2 diabetes mellitus patients,especially complicating chronical complication are higher;the emotion of depression and anxious is related to the life events,especially the negative life events;It is concerned with unstable personality,coping style and the degree of self-esteem.
TOPLUMA HİZMET UYGULAMALARI DERSİNİN DEĞERLENDİRİLMESİ (2012)

Bu calismanin amaci, topluma hizmet uygulamalari (THU) dersinin, paydas goruslerini esas alarak degerlendirmesini yapmaktir. 2006 yilindan itibaren zorunlu okutulan THU dersinin amaci ogretmen adaylarina toplumsal duyarlilik ve ogretmenlik mesleginin duygusal boyutunu kazandirmaktir. Bu calisma katilimci/isbirlikci degerlendirme modeli kullanilarak yapilmistir. Arastirmanin evrenini Kilis 7 Aralik Universitesi’nde dersi alan 200 ogretmen adayi, orneklemini anketin uygulandigi 100 ogretmen adayi olusturmaktadir. Ayrica 34 ogretmen adayi, 11 kurum/kurulus yetkilisi ve 7 ogretim elemaniyla gorusulmustur. THU dersinin islenisinde ortaya cikan temel sorunlarin resmi izinler, plansizlik, maddi kaynaklarin saglanamamasi, iletisim problemleri ve olcme degerlendirme konularinda yogunlastigi tespit edilmistir. Dersin daha iyi islenebilmesi icin danisman ogretim elemanlarinin projenin yapildigi kurum/kuruluslara giderek calismalara ogretmen adaylariyla birlikte daha fazla katilmasi, valilikte bir THU koordinatoru belirlenmesi, dersin paydaslarinin bir araya gelerek donem calisma plani olusturmasi, proje tanitiminin ve sonuclarinin universitenin internet sayfasinda ve yerel basinda tanitilmasi ve degerlendirmenin not ile degil performansa dayali basarili/basarisiz seklinde yapilmasinin uygun olacagi sonucuna ulasilmistir.
Basis Functions for Divergence Constraints in the Finite Element Method (2010)

A method for implementing divergence constraints in the finite element method is given. This method yields a linearly independent, sparse set of constraints for a mesh of brick elements. The method is used to obtain the resonant frequencies of rectangular cavities with perfectly conducting walls. There is good agreement with exact values and there are no spurious modes.
Relating Electrospray Ionization Response to Nonpolar Character of Small Peptides (2000)

Nonpolar regions in biological molecules are investigated as a determining factor governing their electrospray ionization (ESI) mass spectrometric response. Response is compared for a series of peptides whose C-terminal residue is varied among amino acids with increasingly nonpolar side chains. Increased ESI response is observed for peptides with more extensive nonpolar regions. The basis for this increase is examined by comparing values of nonpolar surface area and Gibbs free energy of transfer for the different amino acid residues. Comparisons of response with octadecylamine are also made, and this highly surface-active ion is observed to outcompete all other analytes in ESI response. These observations are rationalized on the basis of the equilibrium partitioning model, which is used successfully to fit experimental data throughout the concentration range for several two-analyte systems. This model suggests that because excess charge exists on ESI droplet surfaces, an analyte's relative affinity for the droplet surface determines its relative ESI response. Increased nonpolar character, which leads to enhanced affinity for the surface phase, results in more successful competition for excess charge and higher ESI response.
Response of <i>Bacillus</i> Spores to Combinations of Germinative Compounds (1966)

Foerster, Harold F. (University of Texas, Austin), and J. W. Foster. Response of Bacillus spores to combinations of germinative compounds. J. Bacteriol. 91:1168-1177. 1966.-Spores of 21 strains of Bacillus megaterium and 25 other strains representing 13 species of Bacillus were produced under standardized conditions. The germination of a washed spore suspension of each strain was measured as a response to various combinations of 30 different germinative compounds. The strains were first typed with respect to their response to "primary" germination compounds, i.e., glucose, l-alanine, inosine, and l-alanine-inosine mixture, and also Na(+) and K(+). The second stage was the determination of the response to various organic and inorganic anions and cations, each strain being supplied with the "primary" compounds best for it. Marked differences in germination patterns were observed among species and strains of the same species. No relation to established taxonomic lines was evident. A nonspecific requirement for ions was found for all strains, but not all ions were effective. A striking degree of interchangeability of germinative chemicals was found. "Fractional germination" was very common. A mixture of l-alanine and inosine and various ions was the best germinative solution for most strains. Some anomalous germination patterns were encountered. Those studied included a strain whose cells lysed spontaneously upon germination and other strains for which l-leucine had striking germinative powers.
Linker-Directed Vertex Desymmetrization for the Production of Coordination Polymers with High Porosity (2010)

Five non-interpenetrated microporous coordination polymers (MCPs) are derived by vertex desymmetrization using linkers with symmetry inequivalent coordinating groups, and these MCPs include properties such as rare metal clusters, new network topologies, and supramolecular isomerism. Gas sorption in polymorphic frameworks, UMCM-152 and UMCM-153 (based upon a copper-coordinated tetracarboxylated triphenylbenzene linker), reveals nearly identical properties with BET surface areas in the range of 3300-3500 m(2)/g and excess hydrogen uptake of 5.7 and 5.8 wt % at 77 K. In contrast, adsorption of organosulfur compounds dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (DMDBT) shows remarkably different capacities, providing direct evidence that liquid-phase adsorption is not solely dependent on surface area or linker/metal cluster identity. Structural features present in MCPs derived from these reduced symmetry linkers include the presence of more than one type of Cu-paddlewheel in a structure derived from a terphenyl tricarboxylate (UMCM-151) and a three-bladed zinc paddlewheel metal cluster in an MCP derived from a pentacarboxylated triphenylbenzene linker (UMCM-154).
Compact and low-loss integrated box-like passband multiplexer (2003)

A fully packaged 40-channel 100-GHz-spacing full-coupler-type synchronized-gratings multiplexer with a 1-dB bandwidth >57 GHz, total adjacent crosstalk /spl plusmn/35 GHz around the ITU grid <-18 dB, and loss <3.5 dB for all channels is presented.
Post injection transmission scanning in a volume imaging PET camera (1994)

Quantitative, low noise, measured attenuation correction has been established for the PENN PET 240H Volume Imaging Camera. This is achieved, without septa, using a narrow energy (450-570 keV) and sinogram position (2 cm wide mask) gate to minimize scatter contamination. Twelve minute transmission acquisitions with a 0.5 mCi rod source are adequate for this purpose. Post injection transmission scans (with emission activity in the FOV) suffer from emission contamination simulating transmitted gamma ray flux. This emission contamination may be measured by performing a transmission acquisition with normal transmission energy and position gating but without a transmission source. This contamination is then subtracted from the measured post injection transmission scan. Emission activity within the FOV adds to detector deadtime, whereas only a small fraction of events are accepted into the transmission position gate, resulting in a net loss of scan statistics when compared to pre-injection transmission scanning. Removal of emission contamination and compensation for this excess deadtime results in corrected attenuation coefficients close to pre-injection values. For residual activity levels typical of FDG whole-body cancer and cardiac studies the post injection measurements are within 4% of pre-injection values.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Intersubject Synchronization of Cortical Activity During Natural Vision (2004)

To what extent do all brains work alike during natural conditions? We explored this question by letting five subjects freely view half an hour of a popular movie while undergoing functional brain imaging. Applying an unbiased analysis in which spatiotemporal activity patterns in one brain were used to "model" activity in another brain, we found a striking level of voxel-by-voxel synchronization between individuals, not only in primary and secondary visual and auditory areas but also in association cortices. The results reveal a surprising tendency of individual brains to "tick collectively" during natural vision. The intersubject synchronization consisted of a widespread cortical activation pattern correlated with emotionally arousing scenes and regionally selective components. The characteristics of these activations were revealed with the use of an open-ended "reverse-correlation" approach, which inverts the conventional analysis by letting the brain signals themselves "pick up" the optimal stimuli for each specialized cortical area.