gebhart/patrepeval huggingface dataset:
Conformational Changes of the Reactive‐Centre Loop and β‐Strand 5A Accompany Temperature‐Dependent Inhibitor‐Substrate Transition of Plasminogen‐Activator Inhibitor 1 (1996)

We have studied conformational changes of type-1 plasminogen-activator inhibitor (PAI-1) during a temperature-dependent inhibitor-substrate transition by measuring susceptibility of the molecule to non-target proteinases. When incubated at 0 degree C instead of the normally used 37 degrees C, a tenfold decrease in the specific inhibitory activity of active PAI-1 was observed. Accordingly, PAI-1 was recovered in a reactive-centre-cleaved form from incubations with urokinase-type plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) at 0 degree C, but not at 37 degrees C. It thus behaved as a substrate for the target proteinases at the lower temperature. Active PAI-1 was exposed to a variety of non-target proteinases, including elastase, papain, thermolysin, trypsin, and V8 proteinase. It was found that specific peptide bonds in the reactive centre loop (RCL) and strand 5 in beta-sheet A (s5A) had a temperature-dependent proteolytic susceptibility, while the P17-P16 (E332-S333) bond, forming the hinge between s5A and the RCL, showed indistinguishable susceptibility to proteolysis by V8 proteinase at 0 degree and 37 degrees C. In latent and reactive-centre-cleaved PAI-1, all the bonds were resistant to proteolysis at the higher as well as the lower temperature. An anti-PAI-1 monoclonal antibody maintained the inhibitory activity of PAI-1 and prevented reactive centre cleavage at 0 degree C, and thus prevented substrate behaviour. Concomitantly, it caused specific changes in proteolytic susceptibility of s5A and the RCL, but it did not affect cleavage of the P17-P16 bond by V8 proteinase. Our observations suggest that temperature-dependent conformational changes of beta-sheet A and the RCL determine whether the serpin act as an inhibitor or a substrate. Furthermore they suggest that the RCL of PAI-1 is fully extracted from beta-sheet A in the inhibitory as well as in the substrate form, favoring a so-called induced conformational state model to explain why inhibitory activity requires partial insertion of the RCL into beta-sheet A.
Streaming Video in Wireless Networks : Service and Technique (2001)

The purpose of this thesis is to present an attractive service for the third generation mobile network that includes streaming video. A prototype application for this service is to be built. The technique behind streaming video is to be presented so that it comes clear what kind of problems and solutions that are associated with streaming. Finally, a platform for streaming video is to be tested and evaluated through different channels. The attractive service presented in this thesis is MMS - Multimedia Messaging Service. Today's popular SMS is evolving beyond text to multimedia. Multimedia will be part of the next generation messaging service called MMS, which will, in an advanced shape, include streaming video. MMS is expected to be a successful service for the next generation?s cell phones. The WAP Forum and the 3GPP industry groups are responsible for standardizing MMS. The standard defines an MMS architecture, which has a number of key elements that interact with each other. The prototype application that was built is called mVideo Messaging and is an MMS that is built on the basis of the MMS standard. The kernel of the prototype is a platform from PacketVideo that makes it possible to stream video over wireless networks. Theories and tests makes it clear that the parameters affecting the video quality can be found at the source/target while coding and compressing, as well as at the streaming-channel. At the channel there are above all three network problems - packet loss, end-to-end delay and delay jitter. To deal with these matters, new protocols have been developed. At the source/target it is important use an efficient compression scheme. MPEG-4 is a new compression scheme that suits very well for streaming video through wireless channels. MPEG- 4 make use of scalability, is object oriented, and is optimized for streaming between 9,6 kbps and 4 Mbps. The service proposed in this thesis as a future service for 3G is practicable. It is also shown that the service can be built using the technology of today.
Angiogenesis-directed implantation of genetically modified endothelial cells in mice. (1995)

By virtue of their location within blood vessels and their ability to express foreign genes, endothelial cells are attractive vehicles for the delivery of therapeutic molecules in vivo. We wished to determine whether i.v.-injected, genetically modified endothelial cells can become incorporated into sites of active angiogenesis in vivo. To do so, we studied the fate of i.v.-injected, lacZ-expressing human umbilical vein endothelial cells in athymic nude mice bearing lethally irradiated NIH 3T3 murine fibroblast cells transfected with a sp-hst/KS3:fibroblast growth factor-1 chimera that forces the secretion of the angiogenic protein, fibroblast growth factor-1. Following i.v. injection, lacZ-labeled human umbilical vein endothelial cells accumulated at sites of fibroblast growth factor-1-induced angiogenesis, persisting for at least 4 weeks. These results suggest that i.v.-administered, genetically modified endothelial cells can migrate into and survive within an angiogenic site. This strategy may be useful for delivery of therapeutic molecules to sites of pathological angiogenesis during tumor metastasis.
An Empirical Study of Consumer Switching from Traditional to Electronic Channels: A Purchase-Decision Process Perspective (2004)

This paper examines the relationship between purchase decisions and channel-switching intentions. A theoretical model that explains channel-switching intentions was tested with a sample of 337 consumers. The results show a 52 percent tendency to switch from off-line to on-line across four product categories: books, airline tickets, wine, and stereo systems. The order of the switching tendency was consistent with the products' search and experience attributes. Logistic regression analysis across product categories shows that differences in channel-risk perceptions, price-search intentions, evaluation effort, and waiting time have a significant impact on consumer switching from off-line to online shopping. Consumers who purchase on-line perceive significantly lower channel risk, search effort, evaluation effort, and waiting (delivery) time on-line than off-line and express significantly higher price-search intentions on-line than off-line. Consumers attracted to off-line channels also perceive lower search-cost and higher price-search intentions online than off-line, but their perceived on-line search effort and price-search intentions are significantly lower than for consumers attracted to on-line channels. These results support the important influence of the examined factors on channel switching. They also suggest that demographics may not be an effective basis for market segmentation.
Chemical modification of sputtered amorphous-carbon surfaces (2001)

Methods to chemically passivate the surfaces of amorphous-carbon films (a-C) produced by dc magnetron sputtering were studied. The chemical composition of carbon surfaces produced via sputtering are dependent upon the environment to which the carbon is exposed immediately following deposition. When the sputtered film is vented to ambient conditions, free radicals produced at the surface during the deposition process are quenched by reaction with oxygen and/or water to form an oxidized, hydrophilic surface. If the sputtered carbon film is, however, exposed to a reactive gas prior to venting to ambient, the chemical nature of the resulting surface can be modified substantially. Specifically, a less highly oxidized and much more hydrophobic carbon surface is produced when the surface free radicals are quenched via either an addition reaction (demonstrated with a fluorinated olefin) or a hydrogen abstraction reaction (demonstrated with two alkyl amines). Chemical modification of amorphous-carbon films can also be accomplished by performing the sputtering in a reactive plasma formed from mixtures of argon with molecular hydrogen, amines, and perfluorocarbons. The elemental composition of these films, and the relative reactivity of the surfaces formed, were investigated via x-ray photoelectron spectroscopy and contact-angle goniometry, respectively. In the case of sputtering with a mixture of argon and hydrogen, increasing the hydrogen flow results in an increase in the amount of hydrogen incorporated into the carbon film and a decrease in the surface free energy. Sputtering in diethylamine produces an amorphous-carbon film into which nitrogen is incorporated. The free energies of the a-C:N surfaces produced in this process are similar to those of the a-C:H films. Sputtering in a fluorocarbon vapor results in the incorporation of fluorine into the film structure and the formation of very low free-energy surfaces. Increasing the concentration of the fluorocarbon in the sputtering plasma increases the amount of F incorporated into the film. At the highest fluorocarbon flow rates employed, a-C films were produced with stoichiometries and surface free energies comparable to those of bulk Teflon.
Resistance to α‐Amylase Digestion in Four Native High‐Amylose Maize Starches (2004)

ABSTRACT Native and processed high‐amylose maize starch (HAMS) is an important source of resistant starch (RS). The objectives of this work were to use an in vitro procedure to estimate the RS content of native granules from a series of ae ‐containing HAMS genotypes, and to examine the nature of the α‐amylase resistant starch (ARS). By the method of Englyst et al (1992), RS for ae V, ae VII, ae su2 , and ae du were estimated to be 66.0, 69.5, 69.5, and 40.6%, respectively. By transmission electron microscopy, most of the residual granules from ae V, ae VII, and ae su2 showed little evidence of digestion. Partially digested granules had a radial digestion pattern in the interior and an enzyme‐resistant layer near the surface. Size and chain‐length profile of constituents of ARS were similar to those of the native HAMS (unlike type 3 RS), consistent with complete hydrolysis in susceptible granule regions. Between crossed polarizers, many iodine‐stained native and residual HAMS granules had blue centers and pink exteriors, which may be due to a difference in orientation of the amylose‐iodine complexes in the exterior. Four granule color types were observed for ae du , differing in enzyme resistance. The high‐enzyme resistance of native HAMS granules may result from altered granule organization, which appears to vary among and within granules from ae ‐containing genotypes.
More than Just Pneumonia: Acute Pulmonary Embolism in Two Middle-Aged Patients with COVID-19 (2020)

The discussion of a hypercoagulable state induced by coronavirus disease 19 has been well documented; however, the exact mechanisms remain unknown. We suspect that a prothrombotic inflammatory response provoked by coronavirus disease could be the culprit, acting as an additive effect on middle-aged patients with known risk factors for venous thromboembolism. We recommend clinicians closely monitor those with known risk factors for pulmonary embolism.
Porosity-Thermal Conductivity Relationships in Plasma Sprayed Zirconia Coatings (2000)

Abstract Thermal barrier coatings (TBCs) are used on heat engine parts to impart protection to components against failure under excessive heat loads, to increase inlet temperatures with consequent improvements in efficiency, and to reduce requirements for cooling. Control of thermal conductivity is addressed since low thermal conductivity depends not only on the nature of the yttria stabilized zirconia (YSZ) layer, but also on the morphology of pores and cracks, which are closely linked to process parameters. This paper will present the influence of feedstock characteristics (particle size distribution and powder morphology) and thermal cycling on porosity content and thermal conductivity of zirconia coatings. The results show increased porosity with particle size, due to an increase in the degree of particle fragmentation and unmelted particles, leading to lower thermal conductivity. Coatings sprayed with powders of different powder morphology yielded changes in porosity and interlamellar contact, thus, influenced thermal conductivity. Sintering effects during thermal cycling resulted in reduced porosity and increased thermal conductivity.
Modification of MyD88 mRNA Splicing and Inhibition of IL-1β Signaling in Cell Culture and in Mice with a 2′- <i>O</i> -Methoxyethyl-Modified Oligonucleotide (2006)

A number of proinflammatory cytokines, including IL-1beta, signal through the adaptor protein MyD88. This signaling leads to phosphorylation of IL-1R-associated kinase-1 (IRAK-1) and, ultimately, activation of the NF-kappaB transcription factor. A splice variant of MyD88 (MyD88(S)), which lacks the ability to couple IRAK-1 to NF-kappaB, has been described. A chemically modified antisense oligonucleotide (ASO) that alters the splicing ratio of MyD88 to MyD88(S) in both cell culture and in animals has been identified. The ASO (ISIS 337846) binds to exon II donor sites in the MyD88 pre-mRNA. By manipulating levels of MyD88 splicing, proinflammatory signaling through the IL-1R has been shown to be diminished, both in cell culture and in mouse liver. To our knowledge, this represents the first example of modulation of RNA splicing of an endogenous gene target in animals after systemic ASO dosing and suggests that this mechanism may be useful as a novel modulator of inflammatory stimuli.
Control of Physical Structure of Silica-Alumina Catalyst (1952)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTControl of Physical Structure of Silica-Alumina CatalystK. D. Ashley and W. B. InnesCite this: Ind. Eng. Chem. 1952, 44, 12, 2857–2863Publication Date (Print):December 1, 1952Publication History Published online1 May 2002Published inissue 1 December 1952https://pubs.acs.org/doi/10.1021/ie50516a029https://doi.org/10.1021/ie50516a029research-articleACS PublicationsRequest reuse permissionsArticle Views399Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Band-edge lasers based on randomly mixed photonic crystals (2010)

By employing two-dimensional InGaAsP photonic band-edge lasers, we have experimentally demonstrated that a random mixture of two different photonic crystals (PCs) possesses a new band structure that is intermediate to that of the two host PCs. The photonic band-edges shift monotonically, but with a strong bowing effect, as the mixed PC system is systematically transformed from one PC to the other. The experimental observations are in excellent agreement with finite-difference time-domain simulations and model calculations based on virtual-crystal approximation with compositional disorder effect included.
High‐level expression of functional cGMP‐dependent protein kinase using the baculovirus system (1993)

The understanding of the structure and function of cGMP-dependent protein kinase (cGMP kinase) has been hindered by the difficulty to obtain large quantities of functional enzyme. A recombinant baculovirus encoding bovine cGMP kinase I alpha was constructed and purified. Infected insect cells synthesized large amounts of soluble and biologically active cGMP kinase I alpha representing up to 10% of the total cell extract protein. The recombinant enzyme had an identical apparent molecular mass, cGMP affinity and kinase activity as the native bovine lung enzyme. The high-level expression of functional cGMP kinase I alpha should provide an excellent tool to study further the structure and function of cGMP kinase.
An Intelligent Driver Assistance System (I-DAS) for Vehicle Safety Modelling using Ontology Approach (2010)

This paper proposes an ontology modelling approach for assisting vehicle drivers through safety warning messages during time critical situation. Intelligent Driver Assistance System (I-DAS) is a major component of InVANET[12], which focuses on generating the alert messages based on the context aware parameters such as driving situations, vehicle dynamics, driver activity and environment. I-DAS manages the parameter representation, consistent update /maintenance in XML format while the interpretation of a critical situation is done using ontology modeling. Related safety technologies such as Adaptive Cruise Control, Collision Avoidance System, Lane Departure Warning System, Driver Drowsiness detection system, Parking Assistance System, which generate warnings and alerts to driver continuously, for assistance according to context which is integrated in Vehicle and Vehicle 2 Driver (V2D) communications by DVI(Driver Vehicle Interface) had been applied. The simulation test bed developed using Java framework [21] to generate safety alerts in various driving situations shows the usefulness of this approach. The response time graph for the simulation of context I-DAS is depicted and analysed. The effective performance of the driving scenarios in various modes like day and night for single, 2-way and 4-way road scenario for the best, worst and average cases of simulation had been studied. The system works in VANET scenario, which needs to be adaptive for environment changes and to vary according to the context. The presented approach shows the simulation that can be implemented to all vehicles in real time scenario with promising results.
IANA Considerations for IPv4 Internet Group Management Protocol (IGMP) (2002)

guidance for the IANA to use in assigning parameters for those fields.
Catalyst-Transfer Polycondensation. Mechanism of Ni-Catalyzed Chain-Growth Polymerization Leading to Well-Defined Poly(3-hexylthiophene) (2005)

We studied the mechanism of the chain-growth polymerization of 2-bromo-5-chloromagnesio-3-hexylthiophene (1) with Ni(dppp)Cl2 [dppp = 1,3-bis(diphenylphosphino)propane], in which head-to-tail poly(3-hexylthiophene) (HT-P3HT) with a low polydispersity is obtained and the M(n) is controlled by the feed ratio of the monomer to the Ni catalyst. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectra showed that the HT-P3HT uniformly had a hydrogen atom at one end of each molecule and a bromine atom at the other. The reaction of the polymer with aryl Grignard reagent gave HT-P3HT with aryl groups at both ends, which indicates that the H-end was derived from the propagating Ni complex. The degree of polymerization and the absolute molecular weight of the polymer could be evaluated from the 1H NMR spectra of the Ar/Ar-ended HT-P3HT, and it was found that one Ni catalyst molecule forms one polymer chain. Furthermore, by reaction of 1 with 50 mol % Ni(dppp)Cl2, the chain initiator was found to be a bithiophene-Ni complex, formed by a coupling reaction of 1 followed by insertion of the Ni(0) catalyst into the C-Br bond of the dimer. On the basis of these results, we propose that this chain-growth polymerization involves the coupling reaction of 1 with the polymer via the Ni catalyst, which is transferred intramolecularly to the terminal C-Br bond of the elongated molecule. We call this mechanism "catalyst-transfer polycondensation".
Structure-Function Analysis of the  -Adrenergic Receptor (1988)

A major goal of medicinal chemistry and pharmacology is to understand the molecular basis for the interactions of drugs with their targets. Many drugs act by binding to specific cell-surface receptors, either as agonists, which stimulate receptor function, or as antagonists, which block the action of endogenous agonists. In recent years, it has become clear that cell-surface receptors can be classified into groups on the basis of their ligand-binding characteristics, their mechanisms of signal transduction, and their structural properties (Dixon et al. 1988). One such class of receptors, which binds small molecule or peptide hormones and neurotransmitters, couples through guanine-nucleotide-binding regulatory proteins (G proteins) to modulate the intracellular levels of a variety of second-messenger molecules (Fig. 1). On binding agonists, receptors of this class catalyze the exchange of a bound GDP molecule for GTP on one of several G proteins in the cell membrane. Most receptors appear to associate with...
Polygon Mapping with Two-Dimensional Solubility Parameters (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPolygon Mapping with Two-Dimensional Solubility ParametersIrwin A. WieheCite this: Ind. Eng. Chem. Res. 1995, 34, 2, 661–673Publication Date (Print):February 1, 1995Publication History Published online1 May 2002Published inissue 1 February 1995https://pubs.acs.org/doi/10.1021/ie00041a028https://doi.org/10.1021/ie00041a028research-articleACS PublicationsRequest reuse permissionsArticle Views376Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts