gebhart/patrepeval huggingface dataset:
Comparison of the ability of monoclonal and polyclonal anti-D antibodies to promote the binding of erythrocytes to lymphocytes, granulocytes and monocytes (1988)

Conference Article| October 01 1988 Comparison of the ability of monoclonal and polyclonal anti-D antibodies to promote the binding of erythrocytes to lymphocytes, granulocytes and monocytes ANTHONY H. MERRY; ANTHONY H. MERRY ‖ *The Blood Group Reference Laboratory, Radcliffe Infirmary, Oxford OX2 6HE, U.K. ‖To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar EVA BROJER; EVA BROJER †Department of Serology, Institute of Haematology, Warsaw, Poland Search for other works by this author on: This Site PubMed Google Scholar BARBARA ZUPANSKA; BARBARA ZUPANSKA †Department of Serology, Institute of Haematology, Warsaw, Poland Search for other works by this author on: This Site PubMed Google Scholar ANDREW G. HADLEY; ANDREW G. HADLEY *The Blood Group Reference Laboratory, Radcliffe Infirmary, Oxford OX2 6HE, U.K. Search for other works by this author on: This Site PubMed Google Scholar BELINDA M. KUMPEL; BELINDA M. KUMPEL ‡U.K. Transplant Centre, Southmead Road, Bristol, U.K. Search for other works by this author on: This Site PubMed Google Scholar NEVIN C. HUGHES-JONES NEVIN C. HUGHES-JONES §Institute for Animal Physiology, Babraham, Cambridge, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1988) 16 (5): 727–728. https://doi.org/10.1042/bst0160727 Article history Received: April 04 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation ANTHONY H. MERRY, EVA BROJER, BARBARA ZUPANSKA, ANDREW G. HADLEY, BELINDA M. KUMPEL, NEVIN C. HUGHES-JONES; Comparison of the ability of monoclonal and polyclonal anti-D antibodies to promote the binding of erythrocytes to lymphocytes, granulocytes and monocytes. Biochem Soc Trans 1 October 1988; 16 (5): 727–728. doi: https://doi.org/10.1042/bst0160727 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: EBV, Epstein Barr virus This content is only available as a PDF. © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.
Differential Impact of Immune Escape Mutations G145R and P120T on the Replication of Lamivudine-Resistant Hepatitis B Virus e Antigen-Positive and -Negative Strains (2009)

Immune escape variants of the hepatitis B virus (HBV) represent an emerging clinical challenge, because they can be associated with vaccine escape, HBV reactivation, and failure of diagnostic tests. Recent data suggest a preferential selection of immune escape mutants in distinct peripheral blood leukocyte compartments of infected individuals. We therefore systematically analyzed the functional impact of the most prevalent immune escape variants, the sG145R and sP120T mutants, on the viral replication efficacy and antiviral drug susceptibility of common treatment-associated mutants with resistance to lamivudine (LAM) and/or HBeAg negativity. Replication-competent HBV strains with sG145R or sP120T and LAM resistance (rtM204I or rtL180M/rtM204V) were generated on an HBeAg-positive and an HBeAg-negative background with precore (PC) and basal core promoter (BCP) mutants. The sG145R mutation strongly reduced HBsAg levels and was able to fully restore the impaired replication of LAM-resistant HBV mutants to the levels of wild-type HBV, and PC or BCP mutations further enhanced viral replication. Although the sP120T substitution also impaired HBsAg secretion, it did not enhance the replication of LAM-resistant clones. However, the concomitant occurrence of HBeAg negativity (PC/BCP), sP120T, and LAM resistance resulted in the restoration of replication to levels of wild-type HBV. In all clones with combined immune escape and LAM resistance mutations, the nucleotide analogues adefovir and tenofovir remained effective in suppressing viral replication in vitro. These findings reveal the differential impact of immune escape variants on the replication and drug susceptibility of complex HBV mutants, supporting the need of close surveillance and treatment adjustment in response to the selection of distinct mutational patterns.
SiO/sub 2/-nanoparticles enhancing Si band-edge electroluminescence to nearly lasing actions (2002)

Electroluminescence (EL) at Si bandgap energy is significantly enhanced with the insertion of SiO/sub 2/ nanoparticles in the oxide layer of the metal-oxide-silicon (MOS) structures on n-type Si. The nanoparticles have a size of 12 nm. The measured EL efficiency is enhanced to be near 10/sup -4/. Furthermore, near-lasing actions like the threshold behavior and resonance modes are observed. The reason is because nanoparticles cause the simultaneous localization of electrons and holes, making the process of the phonon-assisted radiative recombination of electron-hole pairs more like two-particle (exciton-phonon) collision than three-particle (electron-hole-phonon) collision. Thus the probability of radiative recombination increases to enhance EL at Si bandgap energy.
Visualization and Communication Tool for Emergency Response (2018)

The current procedure for assessing emergency situational awareness in the USA requires Police, Paramedic and Fire First Responders to go on site. This observation is termed “size up”. It is effective, but not efficient. Using advanced information and communication for size-up would save Responders time and allow them to begin actual response faster- possibly saving lives. The commercial software that exists for size up is not in wide use. We have developed a software application called an Indoor-Outdoor Viewer for size-up for emergencies so that Responders can get information about the incident before they arrive at the site. It is an easy-to-use Viewer that shows building surroundings and their interiors, and allows Responders to mark the map to organize their approach. It will be compatible with real-time emergency data streams.
Physicochemical controls on adsorbed water film thickness in unsaturated geological media (2011)

Adsorbed water films commonly coat mineral surfaces in unsaturated soils and rocks, reducing flow and transport rates. Therefore, it is important to understand how adsorbed film thickness depends on matric potential, surface chemistry, and solution chemistry. Here the problem of adsorbed water film thickness is examined by combining capillary scaling with the Derjaguin‐Landau‐Verwey‐Overbeek (DLVO) theory. Novel aspects of this analysis include determining capillary influences on film thicknesses and incorporating solution chemistry–dependent electrostatic potential at air‐water interfaces. Capillary analysis of monodisperse packings of spherical grains provided estimated ranges of matric potentials where adsorbed films are stable and showed that pendular rings within drained porous media retain most of the “residual” water except under very low matric potentials. Within drained pores, capillary contributions to thinning of adsorbed films on spherical grains are shown to be small, such that DLVO calculations for flat surfaces are suitable approximations. Hamaker constants of common soil minerals were obtained to determine ranges of the dispersion component to matric potential–dependent film thickness. The pressure component associated with electrical double‐layer forces was estimated using the compression and linear superposition approximations. The pH‐dependent electrical double‐layer pressure component is the dominant contribution to film thicknesses at intermediate values of matric potential, especially in lower ionic strength solutions (<10 mol m −3 ) on surfaces with higher‐magnitude electrostatic potentials (more negative than ≈−50 mV). Adsorbed water films are predicted to usually range in thickness from ≈1 to 20 nm in drained pores and fractures of unsaturated environments.
Blind maximum SINR receiver for the DS-CDMA downlink (2002)

We address the problem of downlink interference rejection in a DS-CDMA system. Periodic orthogonal Walsh-Hadamard sequences spread different users' symbols followed by scrambling by a symbol aperiodic base-station specific overlay sequence. The point-to-point propagation channel from the cell-site to a certain mobile station is the same for all downlink signals (desired user as well as the intracell interference). Orthogonality of the underlying Walsh-Hadamard sequences is destroyed by multipath propagation, resulting in multiuser interference if a coherent combiner (the RAKE receiver) is employed. In this paper, we propose a blind linear equalization algorithm which equalizes for the common downlink channel, thus rendering the user signals orthogonal again. A simple code matched filter subsequently suffices to cancel the multiple access interference (MAI) from intracell users. It is shown that the receiver maximizes the signal-to-interference plus noise ratio (SINR) at its output.
Integrated electrochromic iris device for low power and space-limited applications (2014)

We present a micro-electrooptical iris based on the electrochromic polymer poly(3,4-ethylenedioxythiophene). Two ring-shaped concentric polymer-segments embedded in a transparent electrochemical cell form the micro iris. The polymer layers change their absorption when an external voltage is applied. This iris device benefits from the absence of any mechanically moving part. This renders a very slim design possible, which is suited for small integrated camera systems. During operation the polymer maintains its absorbing state without power consumption. Its low driving voltage of maximum 1.5 V is beneficial for battery powered applications. The impact of the iris on the depth of focus and transmission control as well as its dynamical behavior will be addressed.
Methods and Techniques for the Design and Implementation of Domain-Specific Languages (2012)

Domain-Specific Languages (DSLs) are programming language aimed at a particular problem domain, e.g. banking, database querying or website page lay-outs. Through the use of high-level concepts, a DSL raises the level of abstraction and expressive power of the programmer, and reduces the size of programs. This dissertation covers various aspects of the design and implementations of such DSLs. Throughout the project, two DSLs were developed: WebDSL, a language for rapid web application development, and mobl, a DSL for mobile application development. Using these two case studies, the dissertation explores the design space, as well as techniques developed to implement the compiler and IDE for such DSLs. The general design principle applied is syntactic integration and separation of concerns. Rather than using a number of DSLs to build a single application, our approach is to develop a single, integrated DSL that can be used to develop the entire application, while still enabling clear separation of concerns. The result of this integration is static verification -- the ability to instantly be notified when your program is inconsistent, without having to run it. The dissertation covers five aspects of DSL design and implementation: (1) Verification, the ability to verify applications written using the DSL; (2) Coverage, how to ensure that a DSL enables its user to express what he needs to express; (3) Abstractions, the use as well as the definition of abstractions in a DSL; (4) Code generation, techniques for efficiently generating executable code from a DSL; (5) Portability, the ability to generate code from a DSL that is runnable on multiple platforms.
A Chemotactic Peptide from Laminin α5 Functions as a Regulator of Inflammatory Immune Responses via TNFα-mediated Signaling (2005)

Tissue injury triggers inflammatory responses that may result in release of degradation products or exposure of cryptic domains of extracellular matrix components. Previously, we have shown that a cryptic peptide (AQARSAASKVKVSMKF) in the alpha-chain of laminin-10 (alpha5beta1gamma1), a prominent basement membrane component, is chemotactic for both neutrophils (PMNs) and macrophages (Mphis) and induces matrix metalloproteinase-9 (MMP-9) production. To determine whether AQARSAASKVKVSMKF has additional effects on inflammatory cells, we performed microarray analysis of RNA from RAW264.7 Mphis stimulated with AQARSAASKVKVSMKF. Several cytokines and cytokine receptors were increased >3-fold in response to the laminin alpha5 peptide. Among these were TNF-alpha and one of its receptors, the p75 TNFR (TNFR-II), increasing 3.5- and 5.7-fold, respectively. However, the peptide had no effect on p55 TNFR (TNFR-I) expression. Corroborating the microarray data, the protein levels of TNF-alpha and TNFR-II were increased following stimulation of RAW264.7 cells with AQARSAASKVKVSMKF. In addition, we determined that the production of TNF-alpha and TNFR-II in response to AQARSAASKVKVSMKF preceded the production of MMP-9. Furthermore, using primary Mphis from mice deficient in TNFR-I, TNFR-II, or both TNF-alpha receptors (TNFRs), we determined that AQARSAASKVKVSMKF induces MMP-9 expression by Mphis through a pathway triggered by TNFR-II. However, TNF-alpha signaling is not required for AQARSAASKVKVSMKF-induced PMN release of MMP-9 or PMN emigration. These data suggest that interactions of inflammatory cells with basement membrane components may orchestrate immune responses by inducing expression of cytokines, recruitment of inflammatory cells, and release of proteinases.
Adsorption and Reactions of C<sub>6</sub>Hydrocarbons at High Pressures on Pt(111) Single-Crystal Surfaces Studied by Sum Frequency Generation Vibrational Spectroscopy:  Mechanisms of Isomerization and Dehydrocyclization of<i>n</i>-Hexane (2004)

The adsorption geometries and surface reactions of various C(6) hydrocarbons (n-hexane, 2-methylpentane, 3-methylpentane, and 1-hexene) adsorbed on Pt(111) were investigated using sum frequency generation (SFG) surface vibrational spectroscopy. The adsorptions and reactions were carried out in 1.5 Torr of C(6) hydrocarbons in the absence and presence of excess hydrogen (15 Torr) and in the temperature range 296-453 K. At 296 K and in the presence of excess hydrogen, n-hexane and 3-methylpentane adsorbed molecularly on Pt(111) mostly in "flat-lying" geometries. Upon heating the sample up to 453 K, the molecules underwent dehydrogenation to form new surface species in "standing-up" geometries, such as hexylidyne and metallacyclic species. However, 2-methylpentane and 1-hexene were dehydrogenated to metallacyclobutane and hexylidyne, respectively, at 296 K in the presence of excess hydrogen. The dehydrogenated species remained unreacted on the surface upon heating the sample up to 453 K. The absence of excess hydrogen enhanced dehydrogenation of n-hexane and 3-methylpentane to form pi-allyl c-C(6)H(9) and metallacyclohexane, respectively, at 296 K. Upon heating to 453 K, the pi-allyl c-C(6)H(9) species underwent irreversible dehydrogenation, while hexylidyne and metallacyclic species remained unreacted. On the basis of these results, the mechanisms for catalytic isomerization and dehydrocyclization of n-hexane, which are the important "reforming" reactions to produce high-octane fuels over platinum, were discussed.
Error Control Coding for Multi-Frequency Modulation (1990)

Multi-frequency modulation (MFM) has been developed at NPS using both quadrature-phase-shift-keyed (QPSK) and quadrature-amplitude-modulated (QAM) signals with good bit error performance at reasonable signal-to-noise ratios. Improved performance can be achieved by the introduction of error control coding. This report documents a Fortran simulation of the implementation of error control coding into an MFM communication link with additive white Gaussian noise. Four Reed-Solomon codes were incorporated, two for 16-QAM and two for 32- QAM modulation schemes. The error control codes used were modified from the conventional Reed-Solomon codes in that one information symbol was sacrificed to parity in order to use a simplified decoding algorithm which requires no iteration and enhances error detection capability. Bit error rates as a function of SNR and EbN0 were analyzed, and bit error performance waa weighed against reduction in information rate to determine the value of the codes.
Declarative Constraint Framework for SOA Deployment and Configuration (2008)

A generalized declarative constraint framework is proposed in this paper to address challenges of applying constraints to model-driven SOA deployment and configuration. By representing deployment metamodel and domain knowledge as patterns, constraints can be easily described, automatically validated and analyzed. This framework reduces the human-intensiveness and error-proneness in the activity of applying constraints to a SOA deployment topology. We implemented this framework over a model-driven SOA deployment platform leveraging OCL as the declarative constraint language, and verified it in industry deployment scenarios.
Controlling nanotube growth (2001)

In the past ten years or so, the remarkable electrical and mechanical properties of carbon nanotubes have captured the attention of researchers worldwide. This is largely because these novel structures are of significant fundamental interest and could lead to a huge range of potential applications worth billions of dollars. These range from nanoscale electronics and tools to manipulate individual atoms, to exceptionally strong materials, flat-panel displays and hydrogen fuel cells.
Combining Random Gene Fission and Rational Gene Fusion To Discover Near-Infrared Fluorescent Protein Fragments That Report on Protein–Protein Interactions (2014)

Gene fission can convert monomeric proteins into two-piece catalysts, reporters, and transcription factors for systems and synthetic biology. However, some proteins can be challenging to fragment without disrupting function, such as near-infrared fluorescent protein (IFP). We describe a directed evolution strategy that can overcome this challenge by randomly fragmenting proteins and concomitantly fusing the protein fragments to pairs of proteins or peptides that associate. We used this method to create libraries that express fragmented IFP as fusions to a pair of associating peptides (IAAL-E3 and IAAL-K3) and proteins (CheA and CheY) and screened for fragmented IFP with detectable near-infrared fluorescence. Thirteen novel fragmented IFPs were identified, all of which arose from backbone fission proximal to the interdomain linker. Either the IAAL-E3 and IAAL-K3 peptides or CheA and CheY proteins could assist with IFP fragment complementation, although the IAAL-E3 and IAAL-K3 peptides consistently yielded higher fluorescence. These results demonstrate how random gene fission can be coupled to rational gene fusion to create libraries enriched in fragmented proteins with AND gate logic that is dependent upon a protein-protein interaction, and they suggest that these near-infrared fluorescent protein fragments will be suitable as reporters for pairs of promoters and protein-protein interactions within whole animals.
Targeting FGFR/PDGFR/VEGFR Impairs Tumor Growth, Angiogenesis, and Metastasis by Effects on Tumor Cells, Endothelial Cells, and Pericytes in Pancreatic Cancer (2011)

Activation of receptor tyrosine kinases, such as fibroblast growth factor receptor (FGFR), platelet-derived growth factor receptor (PDGFR), and VEGF receptor (VEGFR), has been implicated in tumor progression and metastasis in human pancreatic cancer. In this study, we investigated the effects of TKI258, a tyrosine kinase inhibitor to FGFR, PDGFR, and VEGFR on pancreatic cancer cell lines (HPAF-II, BxPC-3, MiaPaCa2, and L3.6pl), endothelial cells, and vascular smooth muscle cells (VSMC). Results showed that treatment with TKI258 impaired activation of signaling intermediates in pancreatic cancer cells, endothelial cells, and VSMCs, even upon stimulation with FGF-1, FGF-2, VEGF-A, and PDGF-B. Furthermore, blockade of FGFR/PDGFR/VEGFR reduced survivin expression and improved activity of gemcitabine in MiaPaCa2 pancreatic cancer cells. In addition, motility of cancer cells, endothelial cells, and VSMCs was reduced upon treatment with TKI258. In vivo, therapy with TKI258 led to dose-dependent inhibition of subcutaneous (HPAF-II) and orthotopic (L3.6pl) tumor growth. Immunohistochemical analysis revealed effects on tumor cell proliferation [bromodeoxyuridine (BrdUrd)] and tumor vascularization (CD31). Moreover, lymph node metastases were significantly reduced in the orthotopic tumor model when treatment was initiated early with TKI258 (30 mg/kg/d). In established tumors, TKI258 (30 mg/kg/d) led to significant growth delay and improved survival in subcutaneous and orthotopic models, respectively. These data provide evidence that targeting FGFR/PDFGR/VEGFR with TKI258 may be effective in human pancreatic cancer and warrants further clinical evaluation.
Processing of Ameloblastin by MMP-20 (2007)

Ameloblastin (AMBN) cleavage products are the most abundant non-amelogenin proteins in the enamel matrix of developing teeth. AMBN N-terminal cleavage products accumulate in the sheath space between enamel rods, while AMBN C-terminal products localize within rods. We tested the hypothesis that MMP-20 is the protease that cleaves AMBN. Glycosylated recombinant porcine AMBN (rpAMBN) was expressed in human kidney 293F cells, and recombinant porcine enamelysin (rpMMP-20) was expressed in bacteria. The purified proteins were incubated together at an enzyme:substrate ratio of 1:100. N-terminal sequencing of AMBN digestion products determined that rpMMP-20 cleaved rpAMBN after Pro(2), Gln(130), Gln(139), Arg(170), and Ala(222). This shows that MMP-20 generates the 23-kDa AMBN starting at Tyr(223), as well as the 17-kDa (Val(1)-Arg(170)) and 15-kDa (Val(1)-Gln(130)) AMBN cleavage products that concentrate in the sheath space during the secretory stage. We conclude that MMP-20 processes ameloblastin in vitro and in vivo.
Theoretical Prediction of p<i>K</i><sub>a</sub> Values for Methacrylic Acid Oligomers Using Combined Quantum Mechanical and Continuum Solvation Methods (2008)

The acidity constant pKa for polymeric organic acid is expected to be different from its corresponding monomer value due to the change of chemical environment upon polymerization. Thermodynamic cycles were used to determine the free-energy changes for the proton dissociation processes in aqueous solution and the corresponding pKa values for monomer methacrylic acid and several similar carboxylic acids. First-principles calculations and continuum solvation model were used to determine the gas-phase and solvation free energies, respectively. A protocol was developed to use the efficient density functional calculations with B3LYP functional instead of the demanding CBS-QB3 method to determine the gas-phase free energies with relative high accuracy, thus enabling the determination of pKa values for the short oligomers of methacrylic acid. The predicted pKa values for the dimer and trimer of methacrylic acid are higher by about 0.8 pKa units than the predicted monomer value.