gebhart/patrepeval huggingface dataset:
Filter Throughputs for WFC3 SYNPHOT Support (2006)

ABSTRACT To provide support for WFC3 in the SYNPHOT package, I have combined the measurements made of the WFC3 filters into a single throughput curve for each filter. Each filter was scanned twice, to characterize “in band” sensitivity and “out of band” sensitivity. The “out of band” measurements were typically done at much lower resolution than the “in band” measurements, which makes it difficult to combine these measurements when the throughput is changing rapidly in the region of overlap. Fortunately, this transition region from one type of measurement to another does not comprise a significant fraction of the integrated throughput of the filter, so in practice this mismatch in resolution should not affect the interpretation of photometric data or the prediction of count rates in the various observing modes. The purpose of this report is simply to record the details of how the distinct measurements for each filter were merged.
Purification and Properties of Prostaglandin H‐E Isomerase from the Cytosol of Human Brain: Identification as Anionic Forms of Glutathione <i>S</i>‐Transferase (1987)

Prostaglandin H-E isomerase (EC 5.3.99.3) was purified from human brain cytosol. Purification was by ammonium sulfate fractionation, diethylaminoethyl-Sepharose chromatography, gel filtration on a BioGel P-100 column, GSH-agarose chromatography, and MonoQ chromatography. The activity was eluted in two peaks from the MonoQ column, which were designated peaks 1 and 2. The molecular weights of peaks 1 and 2, determined by gel filtration, were 42,000 and 44,000, respectively. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, peak 1 showed two bands at the molecular weights of 24,500 and 25,000, and peak 2 showed a single band at the molecular weight of 25,000, results suggesting that both were dimeric proteins. The pI values of both enzymes were approximately 5.4. The enzymes catalyzed selective conversion of prostaglandin H2 to prostaglandin E2. The Km values for prostaglandin H2 of peaks 1 and 2 were 147 and 308 microM, respectively, and the Vmax values were 380 and 720 nmol/min/mg of protein, respectively. GSH was required for the catalysis of both enzymes, and no other sulfhydryl compounds could support the reaction. A part of glutathione S-transferase (EC 2.5.1.18) was copurified with peaks 1 and 2 of prostaglandin H-E isomerase. Prostaglandin H-E isomerase activity of peak 2 enzyme was competitively inhibited by 1-chloro-2,4-dinitrobenzene, a substrate of glutathione S-transferase. These results suggested that prostaglandin H-E isomerases in human brain cytosol were identical with anionic forms of glutathione S-transferase.
Alternative Schemes for ISO 9000 Harmonization in Europe (1994)

With the increasing use of ISO 9000, claims have surfaced that the process of certification is being carried out in different ways among the countries of Europe. This situation is already leading to a system of trade barriers as the ISO 9000 certificates, and certification bodies, from some countries are being thought of as being better or worse than others. Explains how an attempt is being made to reconcile these differences, and compares it with other possible strategies.
Superfamily-wide portrait of serine hydrolase inhibition achieved by library-versus-library screening (2010)

Serine hydrolases (SHs) are one of the largest and most diverse enzyme classes in mammals. They play fundamental roles in virtually all physiological processes and are targeted by drugs to treat diseases such as diabetes, obesity, and neurodegenerative disorders. Despite this, we lack biological understanding for most of the 110+ predicted mammalian metabolic SHs, in large part because of a dearth of assays to assess their biochemical activities and a lack of selective inhibitors to probe their function in living systems. We show here that the vast majority (> 80%) of mammalian metabolic SHs can be labeled in proteomes by a single, active site-directed fluorophosphonate probe. We exploit this universal activity-based assay in a library-versus-library format to screen 70+ SHs against 140+ structurally diverse carbamates. Lead inhibitors were discovered for ∼40% of the screened enzymes, including many poorly characterized SHs. Global profiles identified carbamate inhibitors that discriminate among highly sequence-related SHs and, conversely, enzymes that share inhibitor sensitivity profiles despite lacking sequence homology. These findings indicate that sequence relatedness is not a strong predictor of shared pharmacology within the SH superfamily. Finally, we show that lead carbamate inhibitors can be optimized into pharmacological probes that inactivate individual SHs with high specificity in vivo.
Bi<i>s</i>-enaminones as precursors for synthesis of novel 3,4-bis(heteroaryl)pyrazoles and 3,6-bis-(heteroaryl)-pyrazolo[3,4-<i>d</i>]pyridazines (2011)

Coupling of bis-enaminones with benzenediazonium chloride and 3-diazo-1,2,4-triazole proved to be convenient routes for the synthesis of novel 3,4-bis(pyrazol-3-yl)pyrazoles and 3,6-bis(heteroaryl)pyrazolo[3,4- d]pyridazines which have not been reported previously. The structures of the products were elucidated on the basis of their spectral properties, elemental analyses and, wherever possible, by alternate synthesis.
A granulocyte colony-stimulating factor from serum-free cultures of RSP-2P3 cells: Its separation from a macrophage colony-stimulating factor and its biological and molecular characterization. (1984)

A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A small amount of monocyte/macrophage CSF (M-CSF) also was separated from the same medium. The production of this M-CSF was increased markedly by bacterial lipopolysaccharides. The M-CSF had an apparent molecular weight of 77,000 in the absence of 0.1% SDS and 49,000 in its presence. The G-CSF was stable against 5 mM dithiothreitol, whereas the M-CSF was slowly inactivated. The two CSFs also differed in their heat-stability and resistance to trypsin. Neuraminidase changed the isoelectric point of both CSFs. Anti-L cell CSF serum severely inhibited the activity of M-CSF but not that of G-CSF. A 1 : 1 mixture of M-CSF and G-CSF developed colonies of the respective types, both in excess of the number predicted. The RSP -2 X P3 G-CSF reported here should prove very useful in the study of differentiation in myeloid stem cells.
Transforming Growth Factor-β <sub>1</sub> Stimulates <scp>l</scp> -Arginine Transport and Metabolism in Vascular Smooth Muscle Cells (2001)

These results demonstrate that TGF-beta(1) stimulates polyamine and L-proline synthesis by inducing the genes that regulate the transport and metabolism of L-arginine. In addition, they show that TGF-beta(1)-stimulated collagen production is dependent on L-proline formation. The ability of TGF-beta(1) to upregulate L-arginine transport and direct its metabolism to polyamines and L-proline may contribute to arterial remodeling at sites of vascular damage.
A bidirectional contactless power transfer system with dual-side power flow control (2012)

This paper proposed a bidirectional contactless power transfer (CPT) system with controllers at both primary and secondary sides to regulate the power flow. By involving energy feedback mode in the converters of both sides, a backward power flow channel from load to source is achieved which helps pumping out the surplus power from the output filter capacitor to the resonant tank at the secondary side and from the resonant tank to the power supply at the primary side. Furthermore, synchronous rectifying and zero current switching technologies are involved to reducing switching losses. Therefore, with the proposed dual-side control method, rapid regulation and high efficiency can be achieved. Simulation and experimental results show that the output voltage can be controlled to be constant against load variation by regulating the power flow direction dynamically.
Conductive polymers: Towards a smart biomaterial for tissue engineering (2014)

Developing stimulus-responsive biomaterials with easy-to-tailor properties is a highly desired goal of the tissue engineering community. A novel type of electroactive biomaterial, the conductive polymer, promises to become one such material. Conductive polymers are already used in fuel cells, computer displays and microsurgical tools, and are now finding applications in the field of biomaterials. These versatile polymers can be synthesised alone, as hydrogels, combined into composites or electrospun into microfibres. They can be created to be biocompatible and biodegradable. Their physical properties can easily be optimized for a specific application through binding biologically important molecules into the polymer using one of the many available methods for their functionalization. Their conductive nature allows cells or tissue cultured upon them to be stimulated, the polymers' own physical properties to be influenced post-synthesis and the drugs bound in them released, through the application of an electrical signal. It is thus little wonder that these polymers are becoming very important materials for biosensors, neural implants, drug delivery devices and tissue engineering scaffolds. Focusing mainly on polypyrrole, polyaniline and poly(3,4-ethylenedioxythiophene), we review conductive polymers from the perspective of tissue engineering. The basic properties of conductive polymers, their chemical and electrochemical synthesis, the phenomena underlying their conductivity and the ways to tailor their properties (functionalization, composites, etc.) are discussed.
Data Base: Structured Techniques for Design, Performance and Management (1980)

Data. Data Administration. Data Dictionary. Data Models. Data Base Design (Conceptual Model). Data Base Design (Logical Model). Data Storage and Access Methods. Hierarchical Data Base Management Systems. Network Data Base Management Systems. Inverted File Data Base Management Systems. Performance Issues.
Address aggregation in mobile ad hoc networks (2004)

Current mobile ad hoc network (MANET) routing protocols operate with networks as flat address spaces, treating all nodes in the network as independent peers. However, these nodes are not always independent; they can form groups due to physical or environmental restraints. By taking advantage of these natural groupings, there is an opportunity to optimize the performance of MANET routing in these settings. This paper examines address aggregation in MANETs, focusing primarily on its ability to reduce routing overhead. We specifically describe the work necessary to implement address aggregation in the ad hoc on-demand distance vector routing protocol (AODV). We found the benefits of aggregation to be quite significant, reducing overhead and delay by 42% to 82% and 30% to 62%, respectively.
Principles of knowledge representation and reasoning: proceedings of the Third International Conference (KR'92) (1992)

Stringently reviewed papers presented at the October 1992 meeting held in Cambridge, Mass., address such topics as nonmonotonic logic; taxonomic logic; specialized algorithms for temporal, spatial, and numerical reasoning; and knowledge representation issues in planning, diagnosis, and natural langu
Antimicrobial agents (1994)

From the Departments of Pediatrics (SLK) and Clinical Pediatrics and Microbiology and Immunology (EOM), Baylor College of Medicine, and the Infectious Diseases Service (SLK) and the C.T. Parker Laboratory (EOM), Texas Children's Hospital, Houston, TX.
Recent insights in nanotechnology-based drugs and formulations designed for effective anti-cancer therapy (2016)

The rapid development of nanotechnology provides alternative approaches to overcome several limitations of conventional anti-cancer therapy. Drug targeting using functionalized nanoparticles to advance their transport to the dedicated site, became a new standard in novel anti-cancer methods. In effect, the employment of nanoparticles during design of antineoplastic drugs helps to improve pharmacokinetic properties, with subsequent development of high specific, non-toxic and biocompatible anti-cancer agents. However, the physicochemical and biological diversity of nanomaterials and a broad spectrum of unique features influencing their biological action requires continuous research to assess their activity. Among numerous nanosystems designed to eradicate cancer cells, only a limited number of them entered the clinical trials. It is anticipated that progress in development of nanotechnology-based anti-cancer materials will provide modern, individualized anti-cancer therapies assuring decrease in morbidity and mortality from cancer diseases. In this review we discussed the implication of nanomaterials in design of new drugs for effective antineoplastic therapy and describe a variety of mechanisms and challenges for selective tumor targeting. We emphasized the recent advantages in the field of nanotechnology-based strategies to fight cancer and discussed their part in effective anti-cancer therapy and successful drug delivery.
Analysis of Volatile Flavor Components in Roasted Peanuts Using Supercritical Fluid Extraction and Gas Chromatography−Mass Spectrometry (1996)

A method for extraction and identification of volatile flavor components in roasted peanuts is described. The method is based on supercritical fluid extraction (SFE), with identification using gas chromatography−mass spectroscopy (GC−MS) in scan mode. Appropriate choice of supercritical fluid density (0.35 g/mL of CO2) and extraction temperature (50 °C), at a pressure of 96 bar, results in selective extraction of compounds associated with roasted flavor rather than nonvolatile lipid material. The compounds examined in this study were hexanol, hexanal, methylpyrrole, benzene acetaldehyde, methylpyrazine, 2,6-dimethylpyrazine, ethylpyrazine, 2,3-dimethylpyrazine, 2,3,5-trimethylpyrazine, 2-furancarboxaldehyde, 2-ethyl-5-methyl- and 2-ethyl-6-methylpyrazine, and 3-ethyl-2,5-dimethylpyrazine. Careful grinding of frozen samples and alternate layering with silanized glass wool in the extraction thimble allows good recovery of the volatiles (>85%) in a single extraction step. Comparison of chromatograms for samples produced over a range of roasting conditions and sensory panel results shows that this method can be used to relate roasting conditions and consumer acceptance of roast quality. Keywords: Supercritical fluid extraction; peanuts; roasting; GC−MS
[Studies on the chemical constituents of Viscum coloratum]. (2000)

Four compounds were isolated from Viscum coloratum (Kom.) Nakai by means of both solvent extraction and chromatographic separation methods. These constituents were identified through spectral analysis of IR, 1H-NMR. 13C-NMR and MS. They are syringin, oleanolic acid, beta-acetylamyrin and homoeriodictyol-7-beta-D-glucoside. It is the first separation from freshly collected Viscum colortum (Kom.) Nakai, which parasitizing on pear tree.
Improvement in toughness and heat resistance of poly(lactic acid)/polycarbonate blend through twin‐screw blending: Influence of compatibilizer type (2012)

Abstract The thermal mechanical properties and morphological changes of modified poly(lactic acid) (PLA) and polycarbonate (PC) polymer blends based on the equal weight fraction of each component were investigated. Several blend samples were prepared by melt processing with a twin screw extruder using both poly(butylene succinate‐ co ‐lactate) (PBSL) and epoxy (EP) as compatibilizers for the PLA/PC binary system. Differential scanning calorimetry (DSC) of PLA/PBSL and PC/PBSL binary blends showed that individual components were immiscible. Scanning electron microscopy (SEM) analysis of these blends revealed the domain size of PBSL was ∼ 0.5–1 μm in PLA/PBSL blend, and reduced to around 0.1 μm in PC/PBSL blend. The notched Izod impact strength (IS) of PLA/PC/PBSL ternary blends increased with PBSL content up to 10 phr PBSL due to enhanced interfacial interaction and proper domain size of the dispersed phase on the basis of DMA, DSC, and SEM analysis. The heat deflection temperature (HDT) showed a maximum at 5 phr PBSL, and it dropped with increasing PBSL content which is a ductile polymer. However, the HDT of PLA/PC/EP ternary blends increased considerably with 10 phr EP due to rigid interphase formation, and it increased further with 1 phr quaternary amine catalyst, however, the IS dropped nearly the same as that of unmodified PLA/PC blend. To take advantage of the two compatibilizers, PBSL and EP were added to the PLA/PC blend at 10 phr each plus 1 phr catalyst and both IS and HDT were improved significantly over unmodified PLA/PC pair. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Synthesis of chitosan derivative with dual‐antibacterial functional groups and its antibacterial activity (2015)

ABSTRACT The O‐fumaryl ester (OFTMCS) of N,N,N‐trimethyl chitosan (TMCS) has been synthesized as a water‐soluble chitosan (CS) derivative bearing dual‐functional groups, with the aim of discovering novel CS derivatives with good water solubility and enhanced the antibacterial activity compared with unmodified CS. OFTMCS was characterized by FT‐IR, 13 C NMR, XPS, XRD and Zeta potential analyses. The XPS results indicated that the degree of substitution (DS) on the C 2 ‐NH 2 group of the CS was 0.78, and that the DS on its C 6 ‐OH group was 0.31. The TGA results showed that the thermal stability of OFTMCS was lower than that of unmodified CS. The antibacterial activities of OFTMCS were investigated by assessing the mortality rates of the representative Gram‐positive and Gram‐negative bacteria Staphylococcus aureus and Escherichia coli , respectively. The results indicated that OFTMCS exhibited superior antibacterial activity to CS at a lower dosage. The synthesis of CS derivatives bearing dual‐functional groups could therefore be used as a promising strategy to enhance the antibacterial activity of CS. The antimicrobial mechanism of action of OFTMCS was discussed. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42663.
Fibrinogen induces endothelial cell adhesion and spreading via the release of endogenous matrix proteins and the recruitment of more than one integrin receptor (1990)

We have previously shown that fibrinogen (fg) acts as a subendothelial matrix protein in promoting human endothelial cell (EC) adhesion and cytoskeletal organization. In this study we report that EC spreading on fg, at variance with other matrix proteins, requires endogenous matrix protein synthesis and secretion. ECs, upon seeding on fg, promptly released and organized a fibronectin (fn) matrix. Fg was more effective than vitronectin (vn) in promoting the deposition of this protein. ECs treated with monensin to block matrix protein secretion still adhered to fg but did not properly organize their cytoskeleton and adhesion structures. In contrast, monensin did not affect EC spreading either on vn or on fn. Using antibodies to the alpha and beta chains of fn (alpha 5 beta 1) and vn (alpha v beta 3) receptors, it was found that ECs adherent to fg show clustering and organization in adhesion structures of both type of receptors. A faint staining of adhesion structures with alpha 2 but not alpha 3 and alpha 6 antibodies was also observed. Antibodies either to vn or fn receptors were able to disrupt the EC monolayer and to induce EC retraction and detachment, thus indicating that both receptors are important in maintaining a sustained EC adhesion to fg. However, when ECs were treated with monensin only the vn receptor was organized in adhesion structures while the fn receptor was diffusely distributed. This suggests that clustering of the fn receptor is mediated by the release of endogenous matrix proteins induced by the exposure to fg. In conclusion, fg has a peculiar and complex type of interaction with ECs since it requires endogenous matrix protein release and the recruitment of more than one adhesive receptor. This suggests a specific way of response of ECs to each extracellular matrix component.
Performance analysis of a CC-NUMA operating system (2002)

The performance of a CC-NUMA system heavily depends on the amount of interconnection traffic. To reduce the number of remote accesses, an operating system needs to exploit the potential locality of the architecture. In this paper, we present a UNIX-based operating system enhanced for CC-NUMA. The kernel data structures and algorithms are revised for the architecture. We analyze the performance of the enhanced kernel by running SDET and AIM VII commercial benchmarks on a real CC-NUMA system.
Dynamic Software Evolution and The K-Component Model (2001)

There are known classes of software systems that can benefit from dynamic software\nevolution, including 24x7 systems that require on-line upgrades and adaptive systems that need to\nadapt to frequent changes in their execution environment. This paper investigates the use of\ndynamic software architectures and architectural reflection in building adaptive systems. We\nintroduce the K-Component model and its architecture meta-model for building a dynamic\nsoftware architecture. We address the issues of the integrity and safety of dynamic software\nevolution by modelling dynamic reconfiguration as graph transformations on a software\narchitecture, and cleanly separate adaptation-specific code from functional code by encapsulating it\nin reflective programs called adaptation contracts. The paper also introduces the prototype\nimplementation of our K-Component model.
Experimental analysis of adaptive optics compensation in free-space coherent laser communications (2016)

In this work we present a practical, experimental analysis of the effects of adaptive optics compensation on the performance of free-space coherent optical receivers. In order to fulfill this objective, we have developed a laboratory test bed for simulating atmospheric turbulence using Kolmogorov statistics; we have implemented a digital-signal-processing-based phase shift keying heterodyne coherent receiver; and we have integrated a compact module operating a low-cost adaptive optics system that applies modal and zonal wavefront correction. We have checked our experimental results against previously reported analytical models describing the performance of coherent receivers using atmospheric compensation techniques.
Residual Solvents in Biodegradable Microparticles. Influence of Process Parameters on the Residual Solvent in Microparticles Produced by the Aerosol Solvent Extraction System (ASES) Process (1997)

Different batches of microparticles were produced by the aerosol solvent extraction system (ASES) utilizing the extraction properties of supercritical carbon dioxide. Using a central composite design, the influence of production conditions such as spraying rate of the polymer solution and pump rate of the circulating carbon dioxide phase, on the characteristics of microparticles was studied. The experiments were carried out with regard to the residual solvent in the particles, the residual methylene chloride in the carbon dioxide flow of the ASES, the particle size, the morphology, and the yield. The results showed an influence of the pump rate of carbon dioxide on the yield, on the residual solvent in the microparticles, and on the circulating carbon dioxide. There was no major influence of the production conditions on the size and morphology of the microparticles, indicating that the ASES process seems to be a precipitation process.