gebhart/patrepeval huggingface dataset:
Long-Term Effects of Postovulatory Aging of Mouse Oocytes on Offspring: A Two-Generational Study1 (1999)

Aims of this study were to analyze the long-term effects of postovulatory aging of mouse oocytes on 1) reproductive traits of parental (F(0)) and first (F(1))-generation females (pregnancy rate, gestation length, litter size, perinatal death, and sex ratio of offspring) and 2) developmental and behavioral variables of F(1) and second-generation (F(2)) offspring (birth weight and weight gain during preweaning development, postnatal day of attainment of immediate righting, spontaneous motor activity, and passive and active conditioned learning ability). Hybrid (C57BL/6JIco x CBA/JIco) females were artificially inseminated at 13 h (control group) or 22 h (oocyte-aged group) after GnRH injection. Experimental (oocyte-aged group) F(0) females exhibited lower pregnancy rate, shortened gestation length, decreased litter size, higher perinatal death of their pups, and increased percentage of male offspring compared to control F(0) females. Postovulatory aging of oocytes was also associated with increased number of growth-retarded pups, delayed development of the righting reflex, and higher spontaneous motor activity and emotionality of F(1) offspring. Postovulatory aging of F(0) oocytes did not affect birth weight, weight gain during preweaning development, passive and active conditioned learning ability of F(1) offspring, or reproductive traits of F(1) females or developmental and behavior variables of F(2) offspring.
Effect of Polymer Hygroscopicity on the Phase Behavior of Amorphous Solid Dispersions in the Presence of Moisture (2009)

It has been previously observed that exposure to high relative humidity (RH) can induce amorphous-amorphous phase separation in solid dispersions composed of certain hydrophobic drugs and poly(vinylpyrrolidone) (PVP). The objective of this study was to investigate if this phenomenon occurred in solid dispersions prepared using less hygroscopic polymers. Drug-polymer miscibility was investigated before and after exposure to high RH using infrared (IR) spectroscopy and differential scanning calorimetry (DSC). PVP, poly(vinylpyrrolidone-co-vinyl acetate) (PVPVA), and hypromellose acetate succinate (HPMCAS) were selected as model polymers, and felodipine, pimozide, indomethacin, and quinidine were selected as model drugs. Drug-polymer mixing at the molecular level was confirmed for all model systems investigated. Moisture-induced drug-polymer demixing was observed in felodipine-PVPVA, quinidine-PVP, quinidine-PVPVA, pimozide-PVPVA, and pimozide-HPMCAS systems, but was absent in the other HPMCAS dispersions and for indomethacin-PVPVA. It is concluded that the balance between the thermodynamic factors (enthalpy and entropy of mixing) in a ternary water-drug-polymer system is the important factor in determining which solid dispersion systems are susceptible to moisture-induced amorphous-amorphous phase separation. Systems with strong drug-polymer interactions and a less hygroscopic polymer will be less susceptible to moisture-induced phase separation, while more hydrophobic drugs will be more susceptible to this phenomenon even at low levels of sorbed moisture.
Wavelength conversion for frequency-modulated light using optical modulation to oscillation frequency of a DFB laser diode (1990)

A technique for wavelength conversion for frequency-modulated (FM) light is described. It utilizes the light-injection-induced frequency shift of a distributed-feedback (DFB) laser diode and the frequency-modulated-to-intensity-modulated (FM-IM) conversion function of the Mach-Zehnder (M-Z) optical filter. Input FM light is converted into IM light by the M-Z filter. The IM light is injected into the DFB-LD oscillating at another wavelength, modulating its oscillation frequency. The output from the DFB-LD has an FM signal identical to the input FM light. Thus, wavelength conversion for FM light is achieved. The characteristics of the frequency shift induced by this optical injection were experimentally studied using a lambda /4 phase-shift DFB-LD. A wavelength conversion experiment was performed. In the experimental demonstration, FM light at 1.534 mu m was converted into FM light at 1.550 mu m.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Outage performance improvement using relay in device-to-device underlying cellular networks (2014)

Due to the severe interference from the cellular users who share the same spectrum resources in cellular networks, the reliability of D2D communication has been a great concern. Relay has always been considered a importantl tool in improving the quality of wireless system. This paper combines D2D communication system with relay to make D2D communication more reliable. We can conclude from the simulation results that relay does help efficiently to reduce the outage probability of D2D communication by making the D2D received signal-to-interference-and-noise-ratio (SINR) more stable, under the condition that the target SINR of D2D communication is in low regime.
PD-1 Blockade Promotes Emerging Checkpoint Inhibitors in Enhancing T Cell Responses to Allogeneic Dendritic Cells (2017)

Immune checkpoint inhibitors, which target coinhibitory T cell molecules to promote anticancer immune responses, are on the rise to become a new pillar of cancer therapy. However, current immune checkpoint-based therapies are successful only in a subset of patients and acquired resistances pose additional challenges. Finding new targets and combining checkpoint inhibitors might help to overcome these limitations. In this study, human T cells stimulated with allogeneic dendritic cells (DCs) were used to compare immune checkpoint inhibitors targeting TIM-3, BTLA, LAG-3, CTLA-4, and TIGIT alone or in combination with a PD-1 antibody. We found that PD-1 blockade bears a unique potency to enhance T cell proliferation and cytokine production. Other checkpoint inhibitors failed to significantly augment T cell responses when used alone. However, antibodies to TIM-3, BTLA, LAG-3, and CTLA-4 enhanced T cell proliferation in presence of a PD-1 antibody. Upregulation of coinhibitory T cell receptors upon PD-1 blockade was identified as a potential mechanism for synergistic effects between checkpoint inhibitors. Donor-specific variation in response to immune checkpoint inhibitors was attributed to the T cells rather than DCs. Additionally, we analyzed the regulation of checkpoint molecules and their ligands on T cells and allogeneic DCs in coculture, which suggested a PD-1 blockade-dependent crosstalk between T cells and APC. Our results indicate that several immune checkpoint inhibitors have the capacity to enhance T cell responses when combined with PD-1 blockade. Additional <i>in vitro</i> studies on human T cells will be useful to identify antibody combinations with the potential to augment T cell responses in cancer patients.
Nocolok Brazing Aluminum Heat Exchangers (1995)

<div class="htmlview paragraph">Nocolok<sup>®</sup> flux brazing is becoming widely accepted as the principal manufacturing process for brazed aluminum heat exchangers in the automotive industry. This paper summarizes brazing profiles, production results, and quality control details about the Nocolok<sup>®</sup> flux brazing process in relation to its use with heavy duty heat exchangers and oil coolers. The results presented suggest that Nocolok<sup>®</sup> flux brazing is a relatively forgiving process. Acceptable products were brazed using heat rates and temperatures beyond standard requirements suggesting manufacturers will have more flexibility to braze large size heavy duty heat exchangers with the Nocolok<sup>®</sup> process. Pre-brazed gaps of 0.2 mm could be tolerated in the joining of parts while in some cases, gaps up to 0.5 mm were satisfactorily brazed by applying filler alloys such as Al-Si in paste or sheet form to the joint gaps. Material selection and failure modes are discussed in detail as well as relationships between process parameters and scrap rates. The results suggest that component cleanliness and flux loads are critical parameters in the control of production quality and scrap rates.</div>
Magnetostatic interactions between sub-micrometer patterned magnetic elements (2001)

We have investigated the effect of the magnetostatic interaction field between submicrometer patterned magnetic elements in arrays (/spl sim/10/sup 8/ elements) using Alternating Gradient Force Magnetometry. Single layer NiFe elements were studied over a range of width (0.12 to 0.48 /spl mu/m), length to width aspect ratio (1.5 to 4) and thickness (30 /spl Aring/ to 60 /spl Aring/). The arrays were patterned using e-beam lithography in a rectangular lattice with bit separation equal to element width. Effective interaction fields were obtained using a novel application of /spl Delta/M plots to these patterned arrays. The /spl Delta/M plot is derived by subtracting remanent magnetization curves that had initial states of full magnetization from those that were initially demagnetized. Interaction fields were quantified using H/sub int/=/spl Delta/M//spl chi//sub irr/, where /spl Delta/M is the difference in these remanent loops and /spl chi//sub irr/ is the derivative of the remanent magnetization with respect to field. We found H/sub int/ to increase with element aspect ratio, thickness and inverse width. For structures with the largest aspect ratio, thickness and smallest width, H/sub int//spl ap/6 Oe. We calculated the actual dipolar interaction fields using micromagnetic simulation and used these fields in a simple 2-d Ising model to simulate the experiment. We found H/sub int/ that is a good measure of the difference in interaction fields tending to magnetize or demagnetize the sample.
Antigenic mapping of human low density lipoprotein with monoclonal antibodies (1982)

Monoclonal anti-LDL antibodies were produced in a mouse spleen-myeloma system and purified by affinity chromatography on insolubilized low density lipoprotein (LDL). Five antibodies with different specificities could be distinguished by their immunoreactivities with chemically modified LDL preparations, and by their competition for binding to LDL. One of the antibodies inhibited the binding of (125)I-labeled LDL to the apoB,E receptors of cultured human fibroblasts. The same degree of inhibition was achieved using isolated Fab fragments. This antibody may bind to an antigenic site located near the cellular binding site of LDL-apoB.-Tikkanen, M. J., R. Dargar, B. Pfleger, B. Gonen, J. M. Davie, and G. Schonfeld. Antigenic mapping of human low density lipoprotein with monoclonal antibodies.
Effects of GW274150, a novel and selective inhibitor of iNOS activity, in acute lung inflammation (2004)

1. The aim of this study was to investigate the effect of GW274150, a novel, potent and selective inhibitor of inducible nitric oxide synthase (iNOS) activity in a model of lung injury induced by carrageenan administration in the rats. 2. Injection of carrageenan into the pleural cavity of rats elicited an acute inflammatory response characterized by: fluid accumulation in the pleural cavity which contained a large number of polymorphonuclear cells (PMNs) as well as an infiltration of PMNs in lung tissues and subsequent lipid peroxidation, and increased production of nitrite/nitrate (NO(x)), tumour necrosis factor alpha (TNF-alpha) and interleukin-1beta (IL-1beta). 3. All parameters of inflammation were attenuated in a dose-dependent manner by GW274150 (2.5, 5 and 10 mg x kg(-1) injected i.p. 5 min before carrageenan). 4. Carrageenan induced an upregulation of the intracellular adhesion molecules-1 (ICAM-1), as well as nitrotyrosine and poly (ADP-ribose) (PAR) as determined by immunohistochemical analysis of lung tissues. 5. The degree of staining for the ICAM-1, nitrotyrosine and PAR was reduced by GW274150. These results clearly confirm that NO from iNOS plays a role in the development of the inflammatory response by altering key components of the inflammatory cascade. 6. GW274150 may offer a novel therapeutic approach for the management of various inflammatory diseases where NO and related radicals have been postulated to play a role.
Cysteamine revisited: repair of arginine to cysteine mutations (2017)

Cysteamine is a small aminothiol endogenously derived from coenzyme A degradation. For some decades, synthetic cysteamine has been employed for the treatment of cystinosis, and new uses of the drug continue to emerge. In this review, we discuss the role of cysteamine in cellular and extracellular homeostasis and focus on the potential use of aminothiols to reconstitute the function of proteins harboring arginine (Arg) to cysteine (Cys) mutations, via repair of the Cys residue into a moiety that introduces an amino group, as seen in basic amino acid residues Lys and Arg. Cysteamine has been utilized in vitro and ex vivo in four different genetic disorders, and thus provides "proof of principle" that aminothiols can modify Cys residues. Other aminothiols such as mercaptoethylguanidine (MEG) with closer structural resemblance to the guanidinium moiety of Arg are under examination for their predicted enhanced capacity to reconstitute loss of function. Although the use of aminothiols holds clinical potential, more studies are required to refine specificity and treatment design. The efficacy of aminothiols to target proteins may vary substantially depending on their specific extracellular and intracellular locations. Redox potential, pH, and specific aminothiol abundance in each physiological compartment are expected to influence the reactivity and turnover of cysteamine and analogous drugs. Upcoming research will require the use of suitable cell and animal models featuring Arg to Cys mutations. Since, in general, Arg to Cys changes comprise about 8% of missense mutations, repair of this specific mutation may provide promising avenues for many genetic diseases.
Polymers as Rheology Modifiers (1991)

The chapter summarizes the basis of the use of polymers to control or influence fluid rheology and outlines typical applications. Electrorheological fluids, a new class of polymeric rheology modifiers, are discussed.
A Survey of The Problem of Insulator Contamination in The United States and Canada-Part I (1971)

Because of the growing problem caused by insulator contamination due to both industrial pollution and natural deposits, a Working Group of the Lightning and Insulator Subcommittee was formed to study the problem. The first objective was to determine the extent of the problem in the U. S. and Canada, and to determine the various kinds of contaminants and the most severe weather conditions that cause transmission line outages. A survey was conducted by sending questionnaires to 90 utilities. A total of 309 case histories were reported. This report summarizes the answers to questions regarding contaminants and insulator types. It also sheds some light on the preventive measures most frequently undertaken by the utilities and the degree of success of each action.
Cloning and Characterization of a Second Human CTP:Phosphocholine Cytidylyltransferase (1998)

CTP:phosphocholine cytidylyltransferase (CCT) is a key regulator of phosphatidylcholine biosynthesis, and only a single isoform of this enzyme, CCTalpha, is known. We identified and sequenced a human cDNA that encoded a distinct CCT isoform, called CCTbeta, that is derived from a gene different from that encoding CCTalpha. CCTbeta transcripts were detected in human adult and fetal tissues, and very high transcript levels were found in placenta and testis. CCTbeta and CCTalpha proteins share highly related, but not identical, catalytic domains followed by three amphipathic helical repeats. Like CCTalpha, CCTbeta required the presence of lipid regulators for maximum catalytic activity. The amino terminus of CCTbeta bears no resemblance to the amino terminus of CCTalpha, and CCTbeta protein was localized to the cytoplasm as detected by indirect immunofluorescent microscopy. Whereas CCTalpha activity is regulated by reversible phosphorylation, CCTbeta lacks most of the corresponding carboxyl-terminal domain and contained only 3 potential phosphorylation sites of the 16 identified in CCTalpha. Transfection of COS-7 cells with a CCTbeta expression construct led to the overexpression of CCT activity, the accumulation of cellular CDP-choline, and enhanced radiolabeling of phosphatidylcholine. CCTbeta protein was posttranslationally modified in COS-7 cells, resulting in slower migration during polyacrylamide gel electrophoresis. Expression of CCTbeta/CCTalpha chimeric proteins showed that the amino-terminal portion of CCTbeta was required for posttranslational modification. These data demonstrate that a second, distinct CCT enzyme is expressed in human tissues and provides another mechanism by which cells regulate phosphatidylcholine production.
Transposon Tagging and Molecular Analysis of the Maize Regulatory Locus <i>opaque-2</i> (1987)

Genetic analyses suggested that the opaque-2 (o2) locus in maize acts as a positive, transacting, transcriptional activator of the zein seed storage-protein genes. Because isolation of the gene is requisite to understanding the molecular details of this regulation, transposon mutagenesis with the transposable element suppressor-mutator (Spm) was carried out, and three mutable o2 alleles were obtained. One of these alleles contained an 8.3-kilobase autonomous Spm, another a 6.8-kilobase nonautonomous Spm, and the third an unidentified transposon that is unrelated to Spm. A DNA sequence flanking the autonomous Spm insertion was verified to be o2-specific and provided a probe to clone a wild-type allele. Northern blots indicated that the gene is expressed in wild-type endosperm but not in leaf tissues or in endosperms homozygous for a mutant allele of the O2 gene. A transcript was detected in endosperms homozygous for mutations at opaque-7 and floury-2, an indication that O2 expression is independent of these two other putative regulators of zein synthesis.
High-speed BiCMOS technology with a buried twin well structure (1987)

A buried twin well and polysilicon emitter structure is developed for high-speed BiCMOS VLSI's. A bipolar transistor of high cutoff frequency (f <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</inf> = 4 GHz) and small size (500 µm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) has been fabricated on the same chip with a standard 2-µm CMOS, without degrading the device characteristics of the MOSFET. Latchup immunity is improved due to the low well resistance of the buried layer. The well triggering current is a 0.5-1.0 order of magnitude higher than that of a standard n-well CMOS. To evaluate the utility of this technology, a 15-stage ring oscillator of the 2NAND BiCMOS gate is fabricated. The gate has a 0.71-ns propagation delay time and 0.25-mW power dissipation at 0.85-pF loading capacitance and 4-MHz operation. Drive ability is 0.24 ns/pF, which is 2.5 times larger than that of the equal-area CMOS gate.
ChemInform Abstract: Nuclear Synthons: Mesyltriflone as an Olefin Polyanion Equivalent. (1986)

Abstract Sequemial treatment of dimethyl sulfone (I) with ethyl magnesium bromide and lrifyl fluoride (II) gives mesyItri?one (III), which is alkylated via its ma‐dianion to give compounds (V).
<title>Enhanced semi-passive damping using continuous switching of a piezoelectric device on an inductor</title> (2000)

The SSD technique proposed here addresses the problem of resonance damping on a mechanical structure. SSD stands for Synchronized Switch Damping. Apart from active techniques, passive ones consist in connecting a piezoelectric insert attached to the structure to a passive electric network in which the energy generated by the piezoelectric inserts is degraded. In the semi passive approach, the piezoelectric inserts are continuously switched from open circuit to short circuit synchronously to the structure motion. Due to this switching mechanism, a phase shift appears between the piezoelectric strain and the resulting voltage, thus creating energy dissipation. For the new technique proposed here, instead of discharging the piezoelectric inserts during a brief short circuit, they are connected on a small inductor, allowing the inversion of the voltage and then released to open circuit. In this case the voltage amplitude is optimized and is 90 degrees out of phase with the motion then enhancing the damping mechanism. The technique is applicable at any frequency without the need for a large tuned inductor, especially for low frequency applications. There is no need for external power supply unless for the low power circuitry of the switch device. The implementation of the switch drive with a very cheap micro-controller is described. Experimental results measured on cantilever beams made with different materials are proposed. Damping ability ranges from 6 dB on a very viscoelastic epoxy beam to nearly 20 dB on a steel beam. Harmonic excitation and transient results are both proposed and compared. Finally, an electromechanical model is proposed, giving an interpretation of the damping mechanism. Theoretical predictions are in good agreement with the experiments.
Photocatalytic Titanium Dioxide Nanostructures for Self-Regenerating Relative Humidity Sensors (2010)

Relative humidity sensors with a nanostructured titanium dioxide sensing layer were fabricated using glancing angle deposition. Such sensors exhibit very quick response times and high sensitivity but are prone to aging. In previous work, ultraviolet irradiation has been shown to regenerate and improve the sensor performance. Here, the effect of ultraviolet wavelength and power on sensor performance, aging, and regeneration was studied. In order to quantify the change in sensor response a figure of merit (FOM) was defined as the integrated area between the sensor response curves before and after regeneration or aging. FOM values ranged from 2.1 to 61 for treatment with a mercury vapor lamp. Treated sensors aged at an average rate of <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$-$</tex></formula> 0.43 FOM/day. Regeneration of sensors using commercial ultraviolet light-emitting diodes was demonstrated, resulting in FOMs significantly larger than irradiation with comparable mercury vapor lamp wavelengths.
CHARACTERIZATION OF HYDRODYNAMIC LIFT FORCES BY FIELD-FLOW FRACTIONATION. INERTIAL AND NEAR-WALL LIFT FORCES (1994)

Abstract Sedimentation/steric FFF has been used to measure hydrodynamic lift forces exerted on 2-30 μm latex microspheresdnven by flow through a 95 cm long ribbonlike FFF channel of ∼ 127 um thickness. Following a previous study, lift forces are examined as a function of shear rate, distance from the wall, and sphere size. Here, in contrast to the earlier study, measured lift forces are extended downward into a range corresponding to theoretical values of the inertial lift force. After corrections are made for secondary relaxation, it is found, as before, that a near-wall lift force proportional to l/δ (where δ is the particle-wall distance) dominates lift effects at small δ's. As δ increases and this force decays, the measured lift force assumes a value in good agreement with the inertial lift force predicted by the theory of Cox and Brenner as extended by subsequent workers. Over a broad range of conditions explored in almost 300 measurements, the results are consistent with a total lift force that equals the sum of near-wall and inertial contributions. Possible sources of error in the analysis are examined and various explanations for the near-wall lift force are discussed.