gebhart/patrepeval huggingface dataset:
Serum Levels of Surfactant Proteins A and D Are Useful Biomarkers for Interstitial Lung Disease in Patients with Progressive Systemic Sclerosis (2000)

To find a less-invasive and lung-specific clinical biomarker, we measured serum levels of surfactant proteins A and D (SP-A and SP-D) by sandwich enzyme-linked immunosorbent assays in 42 patients with progressive systemic sclerosis (PSS) to evaluate their significance in relation to the presence of interstitial lung disease (ILD) and to assess their diagnostic merits. The patients were divided into two groups based on findings by chest computed tomography (CT): 30 patients with ILD (CT-positive ILD group), and 12 patients without any lung abnormalities (CT-negative ILD group). The CT-positive ILD group was further divided into two groups: 24 patients with ILD detectable by chest plain radiography (X-ray-positive ILD group) and six patients with ILD showing no abnormality (X-ray-negative ILD group). The levels of SP-A and SP-D in sera were significantly higher in the CT-positive ILD group than in the CT-negative ILD group. They were also significantly higher in the X-ray-positive ILD group than in the CT-negative ILD group. In the X-ray-negative ILD group, their levels were higher than those of the CT-negative ILD group. We next estimated sensitivity and specificity of SP-A, SP-D, and X-ray for detecting ILD on CT. Sensitivity of SP-D was high (77%) as well as that of X-ray (80%), whereas SP-A showed a low sensitivity (33%). Remarkably, five of six patients in the X-ray-negative ILD group showed SP-D concentrations over its cut-off level, thereby demonstrating that an SP-D assay contributes to the detection of ILD overlooked by X-ray. Moreover, a combination of X-ray and SP-D dramatically increases sensitivity to 97%. Specificity of SP-A, SP-D, and X-ray to the CT-negative ILD group was 100%, 83%, and 100%, respectively. In conclusion, this study indicates that elevated levels of serum SP-A and SP-D reflect well the presence of ILD and that the combination of SP-D and X-ray contributes to reduce the risk of clinicians overlooking ILD complicated by PSS, although a repetition in another set of subjects is needed to confirm these indications.
A randomized, controlled trial of aprotinin in neonates undergoing open‐heart surgery (2008)

Aprotinin was not shown to be efficacious in neonates undergoing open-heart surgery. It is unclear whether adult aprotinin safety data are relevant to neonates undergoing open-heart surgery.
DESIGN AND SYNTHESIS OF SPECIFIC INHIBITORS OF THE 3′-PROCESSING STEP OF HIV-1 INTEGRASE (2005)

The novel dinucleotide 5'-phosphate, [(L,D)-pIsodApdC], discovered in our laboratory, is a strong inhibitor of HIV-1 integrase for both the 3'-processing and the strand transfer steps. The rationale used in this molecular design was that residues immediately upstream of the dinucleotide cleavage site in the 3'-processing step might provide critical recognition/binding sites on integrase. The rationale for the second type of inhibitors was based on the elimination products (linear and cyclic dinucleotides) of 3'-processing. However, while the linear dinucleotide 5'-phosphate (pdGpdT) was active, its cyclic counterpart was inactive against both wild-type and mutant HIV integrase.
Die an der Verholzung beteiligten Enzyme. Die Dehydrierung des Sinapinalkohols (1958)

Abstract Zur Dehydrierung der p ‐Hydroxy‐zimtalkohole in vitro dient ein Enzymgemisch aus Pilzen (Psalliota) , in dem Laccase der wichtigste Bestandteil ist. Laccase spielt auch die Hauptrolle bei der Dehydrierung des Coniferylalkohols in der Fichte. Die Anreicherung und Wirkung dieser Enzyme wird beschrieben. Kupfersulfat und Sauerstoff sowie Sauerstoff allein bilden aus p ‐Hydroxyzimtalkoholen dieselben Dehydrierungsprodukte wie die Enzyme, wenn auch bedeutend langsamer. Sinapinalkohol wird mit hoher Ausbeute zu Syringaresinol dehydriert.
Disinfection Efficacy in an Integrated Ultraviolet Light Contact Lens Care System (1994)

We evaluated a new integrated contact lens care system that combines fluid turbulence for lens cleaning with ultraviolet (UV) light for solution sterilization. The ultraviolet light system was used to clean and disinfect 42 soft contact lenses (water contents: 38.6%, 43%, 55%, and 70%) and two rigid gas permeable lenses. Test lenses were contaminated with 10(6) cells/mL of Bacillus pumilus, Aspergillus niger, Pseudomonas aeruginosa, and Acanthamoeba castellanii and subjected to a 15-minute cleaning-disinfection cycle. Bathing solutions and contact lenses were cultured at various time intervals and at the end of the cycle. All bathing solutions and all lenses but one were found to be sterile after one cycle. All units effectively disinfected solutions and contact lenses. This device may be an effective alternative to existing contact lens care systems.
Study of control parameters for separation mitigation using an asymmetric single dielectric barrier plasma actuator (2006)

Separation mitigation using asymmetric dielectric barrier discharges is studied by considering the neutral gas flow past a flat plate at an angle of attack. A self-consistent plasma actuator model is employed to couple the electric force field to the momentum of the neutral gas. The equations governing the motion of electrons, ions and neutrals are solved with Poisson's equation to study effective control of flow separation. The impact of select parameters such as amplitude of the excitation, dielectric constants, the initial ionization level and the electrode shape is elucidated. It is found that the dielectric surface just downstream of the exposed electrode becomes negatively charged during part of the cycle for the chosen work parameters and a time averaged force acts on the plasma predominantly downstream, with a transverse component towards the wall. The momentum of the plasma couples to neutral gas through collisions, which results in the enhancement of near-wall momentum yielding a wall-jet feature that effectively eliminates the separation bubble.
Emission spectrometry (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEmission spectrometryMarvin. Margoshes and Bourdon F. ScribnerCite this: Anal. Chem. 1970, 42, 5, 398–417Publication Date (Print):April 1, 1970Publication History Published online1 May 2002Published inissue 1 April 1970https://pubs.acs.org/doi/10.1021/ac60287a030https://doi.org/10.1021/ac60287a030research-articleACS PublicationsRequest reuse permissionsArticle Views47Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Yeast Surface Two-hybrid for Quantitative in Vivo Detection of Protein-Protein Interactions via the Secretory Pathway (2009)

A quantitative in vivo method for detecting protein-protein interactions will enhance our understanding of protein interaction networks and facilitate affinity maturation as well as designing new interaction pairs. We have developed a novel platform, dubbed "yeast surface two-hybrid (YS2H)," to enable a quantitative measurement of pairwise protein interactions via the secretory pathway by expressing one protein (bait) anchored to the cell wall and the other (prey) in soluble form. In YS2H, the prey is released either outside of the cells or remains on the cell surface by virtue of its binding to the bait. The strength of their interaction is measured by antibody binding to the epitope tag appended to the prey or direct readout of split green fluorescence protein (GFP) complementation. When two alpha-helices forming coiled coils were expressed as a pair of prey and bait, the amount of the prey in complex with the bait progressively decreased as the affinity changes from 100 pM to 10 microM. With GFP complementation assay, we were able to discriminate a 6-log difference in binding affinities in the range of 100 pM to 100 microM. The affinity estimated from the level of antibody binding to fusion tags was in good agreement with that measured in solution using a surface plasmon resonance technique. In contrast, the level of GFP complementation linearly increased with the on-rate of coiled coil interactions, likely because of the irreversible nature of GFP reconstitution. Furthermore, we demonstrate the use of YS2H in exploring the nature of antigen recognition by antibodies and activation allostery in integrins and in isolating heavy chain-only antibodies against botulinum neurotoxin.
Une Guyane brésilienne ? (2003)

Existe-t-il une Guyane brésilienne ? Malgré ses similitudes avec les Guyanes voisines, l’Extrême Nord du pays n’est pas perçu comme faisant partie de cet ensemble, mais comme une lointaine périphérie, abandonnée ou laissée en réserve. Mais les changements récents, notamment l’ouverture de nouveaux axes routiers vers les pays voisins, dans le cadre d’une nouvelle politique de développement, change rapidement la région et, tout en y introduisant de fortes différences internes, l’intègre davantage à l’ensemble guyanais.
A role for complement receptor-like molecules in iron acquisition by Candida albicans. (1992)

Candida albicans, an opportunistic fungal pathogen of humans, is dependent upon iron for growth. Consequently, human serum inhibits C. albicans growth due to the presence of high affinity iron-binding proteins that sequester serum iron, making it unavailable for use by the organism. We report that in the inhibitory environment of human serum, the growth of C. albicans can be restored by the addition of exogenous hemoglobin or heme, but not by protoporphyrin IX, the heme precursor that does not contain iron. We further report that C. albicans can utilize cell surface proteins that are homologues of the mammalian complement receptors (CR) to rosette complement-coated red blood cells (RBC) and obtain RBC-derived iron for growth. The ability of Candida to acquire RBC-derived iron under these conditions is dependent upon Candida-RBC rosetting mediated by CR-like molecules. Unopsonized RBC do not support Candida growth in serum, and restoration of Candida growth in serum by complement-opsonized RBC is inhibited by monoclonal antibodies to the human CR type 3 (CR3). In addition, activation of the human alternative pathway of complement by Candida leads to "bystander" deposition of C3 fragments on the surface of autologous, unopsonized RBC, generating the ligands necessary for Candida-RBC rosetting. These results suggest that C. albicans has evolved a unique strategy for acquiring iron from the host, which exploits the host complement system, and which may contribute to the pathogenic potential of the organism.
Role of Neuropoietic Cytokines in Development and Progression of Diabetic Polyneuropathy: From GlucoseMetabolism to Neurodegeneration (2003)

Diabetic neuropathy develops as a result of hyperglycemia-induced local metabolic and microvascular changes in both type I and type II diabetes mellitus. Diabetic neuropathy shows slower impulse conduction, axonal degeneration, and impaired regeneration. Diabetic neuropathy affects peripheral, central, and visceral sensorimotor and motor nerves, causing improper locomotor and visceral organ dysfunctions. The pathogenesis of diabetic neuropathy is complex and involves multiple pathways. Lack of success in preventing neuropathy, even with successful treatment of hyperglycemia, suggests the presence of early mediators between hyperglycemia-induced metabolic and enzymatic changes and functional and structural properties of Schwann cells (SCs) and axons. It is feasible that once activated, such mediators can act independently of the initial metabolic stimulus to modulate SC-axonal communication. Neuropoietic cytokines, including interleukin-1 (IL-1), interleukin-6 (IL-6), leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), tumor necrosis factor alpha (TNF-alpha), and transforming growth factor beta (TGF-beta), exhibit pleiotrophic effects on homeostasis of glia and neurons in central, peripheral, and autonomic nervous system. These cytokines are produced locally by resident and infiltrating macrophages, lymphocytes, mast cells, SCs, fibroblasts, and sensory neurons. Metabolic changes induced by hyperglycemia lead to dysregulation of cytokine control. Moreover, their regulatory roles in nerve degeneration and regeneration may potentially be utilized for the prevention and/or therapy of diabetic neuropathy.
Safety and reliability analysis of wireless data communication concepts for flight control systems (2014)

Wireless communication systems take the place of wired communication systems in various fields to overcome cable-based problems and their limitations. Avionics is one of the fields in which aging and damaging of cables can cause severe accidents. The most important advantages of wireless systems in avionics are weight reduction, resulting in less fuel consumption, flexibility, ease of layout, maintenance and modification. In this contribution, replacement of wired-based safety-critical data communication systems in an aircraft with wireless systems, in terms of safety and reliability is discussed. Under the scope of this work, the conceptual baseline study on wireless flight control systems is carried out. A point-to-point data transmission for a wireless flight control network is simulated considering safety constraints. Therefore, relevant transmission parameters of the wireless system are evaluated, which improve safety level of the wireless transmission. To assess the safety and reliability of the system, failure sources are studied and effects of most influential environmental disturbing factors on the simulated data link are investigated. As a result, effects of changing environmental conditions on the wireless data link for the concept of flight control system are analyzed.
Unsaturated eight-membered ring compounds. XI. Synthesis of sym-dibenzo-1,5-cyclooctadiene-3,7-diyne and sym-dibenzo-1,3,5-cyclooctatrien-7-yne, presumably planar conjugated eight-membered ring compounds (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnsaturated eight-membered ring compounds. XI. Synthesis of sym-dibenzo-1,5-cyclooctadiene-3,7-diyne and sym-dibenzo-1,3,5-cyclooctatrien-7-yne, presumably planar conjugated eight-membered ring compoundsHenry N. C. Wong, Peter J. Garratt, and Franz SondheimerCite this: J. Am. Chem. Soc. 1974, 96, 17, 5604–5605Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://pubs.acs.org/doi/10.1021/ja00824a066https://doi.org/10.1021/ja00824a066research-articleACS PublicationsRequest reuse permissionsArticle Views1168Altmetric-Citations106LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
MPSS: A New Scattering Technique for Sensitive Measuring of the Total Absorption of Fluids (1997)

Optical methods are appropriate tools to detect organic micro-pollutants in fluids. A simple technique is introduced which uses radiation scattered elastically by the fluid. Two different signals are measured: (1) the linear or conventional scattering (CS), which occurs at short pathlengths of the incidence radiation in the fluid, and (2) the multipath-saturation scattering (MPSS) originating at long pathlengths, e.g., in multipath-reflection cuvettes, where the radiation is fully absorbed by the fluid. A relation between the scattering signals and the total absorption coefficient of the fluid is theoretically derived. A simple experiment is performed that takes into consideration the scattering of distilled water polluted with azobenzene and quinine-sulfate. A xenon lamp and a nitrogen laser are used to generate CS and MPSS. The scattering ratio CS/MPSS yields the total absorption directly. In comparison to the conventional absorption technique, CS/MPSS is characterized by a higher sensitivity.
Detection and Quantification of β2AR Internalization in Living Cells Using FAP-Based Biosensor Technology (2010)

Ligand-dependent receptor internalization is a feature of numerous signaling systems. In this article, the authors describe a new kind of live-cell biosensor of receptor internalization that takes advantage of fluorogen-activating protein (FAP) technology. Recombinant genes that express the human beta2 adrenergic receptor (beta2AR) with FAP domains at their extracellular N-termini were transduced into mammalian cells. Exposure of the cells to membrane-impermeant fluorogens led to a strong fluorescent signal from the cell surface. Agonist-dependent translocation of the receptor from the surface to the cell interior was readily observed and quantified by fluorescence microscopy or flow cytometry in a homogeneous format without wash or separation steps. The approach described here is generalizable to other receptors and cell surface proteins and is adaptable to a variety of fluorescence-based high-throughput screening platforms.
Monitoring of recruitment and derecruitment by electrical impedance tomography in a model of acute lung injury (2000)

Electrical impedance tomography is a technique that can show impedance changes resembling recruitment and derecruitment of alveoli in the anterior and posterior parts of the lung. Therefore, electrical impedance tomography may help in determining the optimal mechanical ventilation in a patient with acute lung injury.
Silver Nanoparticles Confined in SBA‐15 Mesoporous Silica and the Application as a Sensor for Detecting Hydrogen Peroxide (2008)

Silver nanoparticles within the pore channels of selectively grafted mesoporous silica SBA‐15 were synthesized. Silanols on the external surface of as‐SBA‐15 were first capped by – groups. After removal of the template of capped SBA‐15 by calcination, silanols on the internal surface of SBA‐15 were modified by 3‐aminopropyltrimethoxysilane (APTMS), and then formaldehyde was grafted by amino groups of APTMS, and further AgSBA‐15). High‐resolution transmission electron microscopy (HRTEM), X‐ray diffraction (XRD), Fourier transformation infrared spectroscopy (FTIR), nitrogen adsorption/desorption isotherms, and UV‐vis spectra confirm that the silver nanoparticles have been confined inside the channels of SBA‐15. In addition, the Ag‐ m SBA‐15 modified electrode (Ag‐ m SBA‐15/GC) exhibited an excellent electrocatalytic activity toward the reduction of hydrogen peroxide (H 2 O 2 ). The proposed H 2 O 2 sensor exhibits a linear range of 48.5 μ M–0.97 M with a detection limit of 12 μ M (S/N = 3 ) and analytical time of 10 seconds per sample.
Tetrathiomolybdate anticopper therapy for Wilson's disease inhibits angiogenesis, fibrosis and inflammation (2003)

The need for agents to lower body copper in Wilson's disease, a disease which results from copper toxicity has been the driving force for the development of the effective anticopper drugs penicillamine, trientine, zinc, and now tetrathiomolybdate (TM). Because of its rapid action, potency, and safety, TM is proving to be a very effective drug for initial treatment of acutely ill Wilson's disease patients. Beyond this, TM has antiangiogenic effects, because many proangiogenic cytokines require normal levels of copper. This has led to use of TM in cancer, where it is generally effective in animal tumor models, and has shown efficacy in preliminary clinical studies. Most recently, it has been found that TM has antifibrotic and antiinflammatory effects through inhibition of profibrotic and proinflammatory cytokines.
Effects of artesunate on cytokinesis and G₂/M cell cycle progression of tumour cells and budding yeast. (2011)

Artesunate, a semi-synthetic derivative of artemisinin, is an effective and safe anti-malaria drug, which also exhibits activity towards cancer cells. The present investigation studied the effect of artesunate on the mitosis of cancer and yeast cells by fluorescence microscopy and mRNA microarrays with a focus on the mitotic spindle checkpoint. The cytotoxicity of artesunate towards seven cell lines from six different cancer types was determined using the XTT assay. Furthermore, the cell cycle distribution of artesunate-treated cells was investigated by flow cytometry and immunofluorescence. To elucidate the genes mediating the effect of artesunate in the mitotic spindle checkpoint, knockout mutants of Saccharomyces cerevisiae were generated, since yeast knockouts are easier to generate than knockout strains of mammalian cells. Four out of the seven tested cell lines showed a G₂/M arrest upon artesunate exposure. Cells residing in the G₂/M arrest revealed multiple centrosomes, small multiple spindles and multi-nucleated cells, suggesting a defect in cytokinesis. The mitotic spindle checkpoint genes bub1, bub2, bub3, mad1, mad2 and mad3 were individually deleted and the sensitivity of these mutants towards artesunate was determined by monitoring the cell growth. The Δbub3 and Δmad3 mutants showed an increased sensitivity and the Δmad2 mutant a slightly decreased sensitivity to artesunate in comparison to the respective wild type. Bub3, Mad3 and Mad2 are the main regulators of the mitotic spindle checkpoint, suggesting that artesunate may interfere with this control mechanism.
Experimental investigation of channel bank vegetation on scouring characteristics around a wing wall abutment (2018)

Local abutment scouring leads to the failure of highway bridges in extreme cases. In this research work, experiments were performed in order to estimate the effect of vegetation on local scouring around abutment under two different scenarios i.e. with and without channel bank vegetation. It was observed that vegetation on channel banks resulted in reduced local scouring around the abutments. The scour depth around abutments with vegetated bank was half of the scour depth as compared to that of non-vegetated banks. The time for attaining the equilibrium condition after sediment scouring in case of vegetated banks was reduced 42% as compared to that of non-vegetated banks.
Blood‐Oxygenation‐Level‐Dependent‐(BOLD‐) Based R2′ MRI Study in Monkey Model of Reversible Middle Cerebral Artery Occlusion (2011)

BOLD-based R2' MRI can be used to describe changes of cerebral oxygen extract in acute ischemic stroke, and it can provide additional information in detecting IP. The time evolution rR2' in infarcted core, IP, and oligemia is in accordance with the underlying pathophysiology.
Cellular Physiology of Cholesterol Efflux in Vascular Endothelial Cells (2004)

Endothelial cells may use nontraditional pathways for cholesterol efflux.