gebhart/patrepeval huggingface dataset:
New insights into form and function of fibronectin splice variants (2008)

The extracellular matrix (ECM) is a highly dynamic structure that not only provides a physical framework for cells within connective tissues, but also imparts instructive signals for development, tissue homeostasis and basic cell functions through its composition and ability to exert mechanical forces. The ECM of tissues is composed of, in addition to proteoglycans and hyaluronic acid, a number of proteins, most of which are generated after alternative splicing of their pre-mRNA. However, the precise function of these protein isoforms is still obscure in most cases. Fibronectin (FN), one of the main components of the ECM, is also one of the best-known examples of a family of proteins generated by alternative splicing, having at least 20 different isoforms in humans. Over the last few years, considerable progress on elucidating the functions of the alternatively spliced FN isoforms has been achieved with the essential development of key engineered mouse strains. Here we summarize the phenotypes of the mouse strains having targeted mutations in the FN gene, which may lead to novel insights linking function of alternatively spliced isoforms of fibronectin to human pathologies.
Network management with Intelligent Trader (2002)

The authors describe the concept of Intelligent Trader and the network management architecture based on it. First, we describe problems in the modern network system, from the viewpoint of network management. Next, we describe the similarity and difference between network management and application integration. Then we discuss the merits and problems of the application integration technique when applying it to network management. Finally, we propose the intelligent trader system which incorporates the application integration technique into the network management.
Selective COX-2 inhibitor reduces bone healing in bone defects (2005)

Anti-inflammatory agents have been reported to regulate bone healing. The aim of this study was to investigate the effect of a selective cyclooxygenase-2 inhibitor (meloxicam) on bone healing in calvarial defects in rats. Thirty-six adult male Wistar rats were included. After anesthesia, a linear incision was made through the skin of the scalp, a full-thickness flap was reflected and a 4 mm round defect was made with a trephine drill. The animals were randomly assigned to one of the following 4 treatment groups (9 animals each), including daily subcutaneous injections: A: saline solution for 15 days; B: saline solution for 45 days; C: 3 mg/kg of meloxicam for 15 days and D: 3 mg/kg of meloxicam for 45 days. The animals were sacrificed and the specimens, routinely processed. The bone filling was histometrically measured and statistical analysis, performed. Intergroup comparisons demonstrated that the meloxicam groups presented a significant reduction in bone healing when compared to their respective controls (group A, 44.5 +/- 5.75%, against group C, 57.5 +/- 7.25%, p < 0.05; group B, 40.25 +/- 13.75%, against group D, 52.25 +/- 17.25%). Within the limits of the present study, it can be concluded that selective cyclooxygenase-2 inhibitors may reduce bone healing in calvarial defects in rats after continuous administration.
Statistical design techniques for high-speed circuit boards with correlated structure distributions (1991)

Statistical design techniques for the line structures of high-speed circuit boards have been developed. These techniques account for dimensional distribution correlations caused by the fabrication process. Monte Carlo simulation is introduced into the finite element method for calculating line parameters. The eye pattern at the receiving end of transmission lines with parallel and stub parts is simulated, using distributed circuit modes, for the calculated line parameter distributions. The simulator has an algorithm to save computer time. The calculated and experimental results are compared, and the accuracy of the simulation is discussed. Calculation results imply that the evaluation for the dimensional distribution of fabrication processes is very important. The timing and amplitude margins were found to strongly depend on fabrication deviations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The actuarial uses of health service indicators for the National Health Service in England: seminar, 8 July 1993 (1993)

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Using Extensible Query Language (XQL) for database applications (2002)

An important application of XML is the interchange of electronic data (EDI) between multiple data sources on the Web. As XML data proliferates on the Web, applications will need to integrate and aggregate data from multiple sources and clean and transform data to facilitate exchange. Data extraction, conversion, transformation, and integration are all well-understood database problems, and their solutions rely on a query language. A query language for XML, called XQL, is discussed, which is suitable for performing the above tasks. XQL is a declarative, "relational complete" query language and is simple enough that it can be optimized. XQL can extract data from existing XML documents and construct new XML documents.
A New Passive Hybrid Air-Core Foil Filter for Modern Power Drives (2010)

This paper introduces an alternative implementation of the traditional <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LC</i> filter, called hybrid <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LC</i> filter (HLCF). The construction described in this paper contains two foil windings that integrate inductance and capacitance into one unit. The main aspects of the HLCF design are considered. The experimental data show that the device proposed here can be used, instead of traditional <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LC</i> filters, as an alternative solution for the mitigation of the adverse effects of the pulse-width modulation (PWM).
Identification of Glu<sup>173</sup> as the critical amino acid residue for the ADP‐ribosyltransferase activity of <i>Clostridium botulinum</i> C3 exoenzyme (1995)

Clostridium botulinum C3 exoenzyme specifically ADP‐ribosylates rho‐p21 in eukaryotic cells. Trp 18 and Glu 173 of this enzyme were substituted with other amino acids via site‐directed mutagenesis. All substitutions at Glu 173 caused a significant reduction in affinity for NAD and diminished ADP‐ribosyltransferase activity. On the other hand, the activity of enzymes with the substitution at Trp 18 remained intact. Swiss 3T3 cells treated with the enzyme with the Trp 18 substitution showed the typical morphologic changes of the C3 exoenzyme phenotype. In contrast, no changes were found in cells incubated with the Glu 173 ‐substituted enzyme. These results indicate that the Glu 173 residue of the C3 exoenzyme plays a key role in interacting with NAD and in expression of ADP‐ribosyltransferase activity, which is essential for the phenotypic change by C3 exoenzyme treatment.
New insights into the t-complex and control of sperm function (1999)

The mouse t-complex, located on chromosome 17, contains genes known to influence male, but not female, fertility. Although some t-complex genes are recessive lethals, t-chromosomes are maintained in the population by transmission ratio distortion. When male mice heterozygous for the t-chromosome mate with wild-type females, most offspring will possess the t-chromosome, indicating a link between t-complex genes and sperm function. Several proteins coded for by t-complex genes have been localised in the sperm flagellum, suggesting roles relating to motility. Another t-complex protein appears able to regulate the adenylyl cyclase/cAMP signal transduction pathway, known to play an important role in capacitation. Defective motility and/or failure to capacitate ("switch on") would result in poorly fertile or infertile spermatozoa. Given the existence of human homologues for many genes in the t-complex and the prevalence of "male factor" infertility, information obtained about the t-complex not only will provide insight into basic biological mechanisms but may be of future clinical relevance as well.
An Analytical Assessment of NASA's N(+)1 Subsonic Fixed Wing Project Noise Goal (2010)

The Subsonic Fixed Wing Project of NASA s Fundamental Aeronautics Program has adopted a noise reduction goal for new, subsonic, single-aisle, civil aircraft expected to replace current 737 and A320 airplanes. These so-called "N+1" aircraft--designated in NASA vernacular as such since they will follow the current, in-service, "N" airplanes--are hoped to achieve certification noise goal levels of 32 cumulative EPNdB under current Stage 4 noise regulations. A notional, N+1, single-aisle, twinjet transport with ultrahigh bypass ratio turbofan engines is analyzed in this study using NASA software and methods. Several advanced noise-reduction technologies are empirically applied to the propulsion system and airframe. Certification noise levels are predicted and compared with the NASA goal.
A method for detecting ECG validity based on wavelet. (2009)

Objective:To investigate the validity of the collected electrocardiogram(ECG) and improve the accuracy of ECG diagnosis and analysis.Methods:Signals were preprocessed by suppressing the noise such as baseline wander,power-line and harmonic interferences and electromygram(EMG).Then,they were calculated by the proposed method with 4 s slipping windows.In order to evaluate the accuracy and general applicability of the algorithm for different ECG waveforms.This algorithm was evaluated on the complete MIT-BIH Arrhythmia Database,some datas from CU Database and the MIT-BIH Noise Stress Test Database.Results:Simulation results show that the method can successfully distinguish the valid and invalid ECG signals.The fallout ratio is below 5%.It is simple and easily implemented.Conclusion:This method has a higher accuracy and reduces the calculation for the subsequent diagnosis,such as ventricular fibrillation.It may serve in the real-time remote health monitor system.
Sensor and network technology for intelligent transportation systems (2012)

The current state of the art advancements in sensors, computation, and wireless communication technologies has made possible a vast range of emerging applications in large number of fields such as in civil and military transportation systems, personal communications, health care, industrial automation, smart grids, smart homes, etc. Even though the automobile does not seem to have changed much its mechanical image since its first introduction, the industry is currently adapting itself in response to the information technology revolution. Thus, today it is not uncommon to see GPS assisted navigation systems, driver assist technology for parallel parking, adaptive cruise control, and lane-departure warning. Technology is also making way into the transportation infrastructure such as in electronic toll collection, traffic monitoring, accident alert systems, etc. This paper reviews the current state of the technology deployed to support intelligent transportation systems and looks into the potential of wireless sensor network technology with the aim to reduce cost and power consumption, while at the same time enhancing flexibility as well as efficiency of service.
Molecular characterization of the amplified carboxylesterase gene associated with organophosphorus insecticide resistance in the brown planthopper, <i>Nilaparvata lugens</i> (2000)

Widespread resistance to organophosphorus insecticides (OPs) in Nilaparvata lugens is associated with elevation of carboxylesterase activity. A cDNA encoding a carboxylesterase, Nl-EST1, has been isolated from an OP-resistant Sri Lankan strain of N. lugens. The full-length cDNA codes for a 547-amino acid protein with high homology to other esterases/lipases. Nl-EST1 has an N-terminal hydrophobic signal peptide sequence of 24 amino acids which suggests that the mature protein is secreted from cells expressing it. The nucleotide sequence of the homologue of Nl-EST1 in an OP-susceptible, low esterase Sri Lankan strain of N. lugens is identical to Nl-EST1. Southern analysis of genomic DNA from the Sri Lankan OP-resistant and susceptible strains suggests that Nl-EST1 is amplified in the resistant strain. Therefore, resistance to OPs in the Sri Lankan strain is through amplification of a gene identical to that found in the susceptible strain.
The Synthesis of Heat-shock Proteins after a Decrease in Translational Capacity in Escherichia coli (1987)

Various conditions which decrease translational capacity and enhance the synthesis of ribosomal components were analysed with respect to the synthesis of heat-shock proteins in Escherichia coli: (a) deprivation of streptomycin from a streptomycin-dependent mutant, (b) addition of tetracycline to a partially tetracycline-resistant strain, and (c) nutritional shift-up conditions. In all cases, the rate of synthesis of the heat-shock proteins DnaK, GroEL and C62.5 decreased while the synthesis of ribosomal components increased. Thus inhibition of ribosome formation or a decrease in translational capacity do not induce the stress proteins, but have the opposite effect.
Isolation from opossum serum of a metalloproteinase inhibitor homologous to human .alpha.1B-glycoprotein (1992)

Fractionation of opossum (Didelphis virginiana) serum with (NH4)2SO4, followed by chromatography on DEAE-Sepharose, phenyl-Sepharose, and Mono Q HR 5/5, has resulted in the isolation in homogeneous condition of a metalloproteinase inhibitor designated oprin (opossum proteinase inhibitor). Oprin is a single-chain glycoprotein (26% carbohydrate) with an estimated Mr = 52,000, pI = 3.5, and E(1%/1 cm) = 11. Oprin inhibited snake venom metalloproteinases, but showed no activity on venom serine proteinases or on bacterial metalloproteinases. Incubation of Crotalus atrox alpha-proteinase (EC 3.4.24.1) with oprin, and analysis of the reaction products by chromatography on Mono Q HR 5/5 and by electrophoresis under nondenaturing conditions, indicated formation of an inactive enzyme/inhibitor complex. The complex dissociated during SDS/polyacrylamide gel electrophoresis. An opossum liver cDNA library was immunoscreened, and clones containing cDNA encoding for part of the open reading frame for oprin were isolated. The cDNA inserts contained nucleotide sequences corresponding to two internal amino acid sequences of oprin which had been separately determined by protein sequence analysis. Protein database screening using a 211 amino acid sequence deduced from one of the cDNA inserts showed no significant homology to known proteinase inhibitors. There was, however, a 36% identity with human alpha 1B-glycoprotein, a plasma protein of unknown function related to the immunoglobulin supergene family. In addition, the amino-terminal sequence of oprin showed 46% identity with human alpha 1B-glycoprotein in a 26 amino acid residue overlap.(ABSTRACT TRUNCATED AT 250 WORDS)
Solid-Phase Synthesis of Indolecarboxylates Using Palladium-Catalyzed Reactions (2003)

Polymer-supported, palladium-catalyzed cyclization reactions effectively synthesized indolecarboxylates. Palladium-catalyzed carbon-carbon bond-forming reactions of immobilized enaminoesters followed by transesterification yielded indole 2- or 3-carboxylates with various functional groups on the benzene ring. Indolecarboxylates were efficiently cyclized via an intramolecular palladium-catalyzed amination reaction of immobilized N-substituted dehydrohalophenylalanines, and immobilized N-acetyl-dehydroalanines were efficiently converted into indolecarboxylates via tandem Heck-amination reactions.
Characterization of a voltage-gated K+ channel beta subunit expressed in human heart. (1995)

Voltage-gated K+ channels are important modulators of the cardiac action potential. However, the correlation of endogenous myocyte currents with K+ channels cloned from human heart is complicated by the possibility that heterotetrameric alpha-subunit combinations and function-altering beta subunits exist in native tissue. Therefore, a variety of subunit interactions may generate cardiac K+ channel diversity. We report here the cloning of a voltage-gated K+ channel beta subunit, hKv beta 3, from adult human left ventricle that shows 84% and 74% amino acid sequence identity with the previously cloned rat Kv beta 1 and Kv beta 2 subunits, respectively. Together these three Kv beta subunits share > 82% identity in the carboxyl-terminal 329 aa and show low identity in the amino-terminal 79 aa. RNA analysis indicated that hKv beta 3 message is 2-fold more abundant in human ventricle than in atrium and is expressed in both healthy and diseased human hearts. Coinjection of hKv beta 3 with a human cardiac delayed rectifier, hKv1.5, in Xenopus oocytes increased inactivation, induced an 18-mV hyperpolarizing shift in the activation curve, and slowed deactivation (tau = 8.0 msec vs. 35.4 msec at -50 mV). hKv beta 3 was localized to human chromosome 3 by using a human/rodent cell hybrid mapping panel. These data confirm the presence of functionally important K+ channel beta subunits in human heart and indicate that beta-subunit composition must be accounted for when comparing cloned channels with endogenous cardiac currents.
Removal of N-Linked Glycosylations at Acidic pH by PNGase A Facilitates Hydrogen/Deuterium Exchange Mass Spectrometry Analysis of N-Linked Glycoproteins (2016)

Protein glycosylation is the most frequent post-translational modification and is present on more than 50% of eukaryotic proteins. Glycosylation covers a wide subset of modifications involving many types of complex oligosaccharide structures, making structural analysis of glycoproteins and their glycans challenging for most analytical techniques. Hydrogen/deuterium exchange monitored by mass spectrometry is a sensitive technique for investigation of protein conformational dynamics of complex heterogeneous proteins in solution. N-linked glycoproteins however pose a challenge for HDX-MS. HDX information can typically not be obtained from regions of the glycoprotein that contain the actual N-linked glycan as glycan heterogeneity combined with pepsin digestion yields a large diversity of peptic N-glycosylated peptides that can be difficult to detect. Here, we present a novel HDX-MS workflow for analysis of the conformational dynamics of N-linked glycoproteins that utilizes the enzyme PNGase A for deglycosylation of labeled peptic N-linked glycopeptides at HDX quench conditions, i.e., acidic pH and low temperature. PNGase A-based deglycosylation is thus performed after labeling (post-HDX) and the utility of this approach is demonstrated during analysis of the monoclonal antibody Trastuzumab for which it has been shown that the native conformational dynamics is dependent on the N-linked glycan. In summary, the HDX-MS workflow with integrated PNGase A deglycosylation enables analysis of the native HDX of protein regions containing N-linked glycan sites and should thus significantly improve our ability to study the conformational properties of glycoproteins.
Don't Mind the Gap (2016)

We develop Propane, a language and compiler to help network operators with a challenging, error-prone task—bridging the gap between network-wide routing objectives and low-level configurations of devices that run complex, distributed protocols. The language allows operators to specify their objectives naturally, using high-level constraints on both the shape and relative preference of traffic paths. The compiler automatically translates these specifications to router-level BGP configurations, using an effective intermediate representation that compactly encodes the flow of routing information along policy-compliant paths. It guarantees that the compiled configurations correctly implement the specified policy under all possible combinations of failures. We show that Propane can effectively express the policies of datacenter and backbone networks of a large cloud provider; and despite its strong guarantees, our compiler scales to networks with hundreds or thousands of routers.
Full-Duplex MAC Protocol Based on Adaptive Contention Window for Visible Light Communication (2015)

As an alternative technology to radio-frequency-based communication, visible light communication (VLC) has attracted tremendous interest from both academia and industry. For its medium access control (MAC) protocol, most existing design has focused on the half-duplex mode, which can be inefficient for two-way communications. In this paper, we propose a novel full-duplex MAC protocol for VLC networks. Different from half-duplex operation, full-duplex operation allows concurrent sending and receiving of data frames between the central node and terminal nodes. We show that under certain scenarios, it can achieve almost threefold downlink throughput from the central node to terminal nodes as compared with half-duplex operation. Unfortunately, owing to asymmetric traffic demand on the uplink and downlink, full-duplex transmission cannot always be realized. To increase the probability of full-duplex transmission, we further propose a self-adaptive minimum contention window full-duplex MAC protocol that can significantly increase the probability of duplex operation. Our experimental study shows that the proposed MAC protocol can effectively mitigate channel collisions and achieve significantly higher system throughput.
Preparation of Amidines from Amidoximes via Transfer Hydrogenation (2011)

Amidoximes are reduced into amidine using triethyl silane and PdCl2 in acetic acid. Keywords: AmidinesamidoximesPdCl2 reductiontriethyl silane ACKNOWLEDGMENT The authors thank Dr. Dhanjay Sathe, associate vice president of USV Limited, for valuable suggestions. Notes Note. Isolated yield: all products were characterized by NMR (1H and 13C).
SPECTRA:  A Spectral Information Management System Featuring a Novel Combined Search Function (1996)

The SPECTRA collection of software as a spectral information management system for organic compound structure determination is described. The SPECTRA (SPECTral Research and Analysis) system suggests candidate structures for chemical compounds based on analysis of their spectra, where mass spectra, infrared spectra, 1H-nuclear magnetic resonance spectra, and 13C-nuclear magnetic resonance spectra are possible input. The system computes the optimal matching of an input spectrum with stored spectra in a database and also retrieves the spectra of compounds that contain a substructure of the unknown compound. A novel combined search algorithm can be activated when two to four spectra are given as information of an unknown compound. Similarities between the input spectrum and each spectrum in the database are calculated, and the corresponding candidate compounds are ranked according to their similarity score.
Power Control of Domestic Microwave Ovens by Switching in the High-Voltage Circuitry (1978)

AbstractThe authors have developed a new power control method, in which a high-voltage switch is used, and the output power is controlled by changing the interval of half-wave current flow time in the secondary circuit of the transformer, The switch is actuated at zero-current (intermittent of half-wave current) by a controller and a phase-control device.With this method, there is no rush current, no electric arc and little magnetron filament temperature change, which results in the increased life of the magnetron.