gebhart/patrepeval huggingface dataset:
Stable and Functional Immobilization of Histidine-Tagged Proteins via Multivalent Chelator Headgroups on a Molecular Poly(ethylene glycol) Brush (2005)

We present a generic approach for immobilizing oligohistidine-tagged proteins with high stability and homogeneous functionality onto glass-type surfaces. Multivalent chelator heads (MCH) carrying two and three nitrilotriacetic acid (NTA) moieties were coupled with controlled surface concentration to glass surfaces premodified with an ultrathin two-dimensional polymer brush of a bifunctional poly(ethylene glycol). Low roughness and lateral homogeneity of these surfaces were confirmed by AFM and fluorescence microscopy, respectively. Protein immobilization and interactions at these interfaces were studied by label-free and fluorescence detection. Oligohistidine-tagged proteins bound specifically to NTA loaded with nickel(II) ions and could be eluted with imidazole. More than 90% of the immobilized protein preserved its activity. In contrast to mono-NTA, immobilized multivalent chelator heads bound oligohistidine-tagged proteins stoichiometrically and with high stability, even at very low chelator surface concentrations. Thus, an excess of the metal chelator sites was not necessary, and excessive binding sites could be quantitatively blocked with an indifferent protein. As a consequence, increased functional stability of the immobilized protein and a substantial reduction in nonspecific adsorption were achieved. Binding of histidine-tagged proteins to the MCH-modified surface was efficiently blocked by stoichiometric amounts of soluble MCH, and biomolecular interaction unbiased by the interaction of the histidine tag to the surface-bound MCH was observed. These excellent features and the compatibility with many solid-phase analytical techniques make this surface chemistry beneficial for functional protein analysis.
Hepatitis B virus X protein suppresses caveolin-1 expression in hepatocellular carcinoma by regulating DNA methylation (2012)

HBx protein induces methylation of the caveolin-1 promoter region and suppresses its expression.
Higher Numbers of T-Bet+ Tumor-Infiltrating Lymphocytes Associate with Better Survival in Human Epithelial Ovarian Cancer (2017)

Our data indicated that the key transcription factor T-bet might play an important role in the type I immune cells mediated antitumor response, and the density of T-bet+ lymphocytes in human epithelial ovarian cancer tissues could serve as a prognostic predictor for ovarian cancer patients.
A Double-Blind Randomized Controlled Trial Comparing Epidural Clonidine vs Bupivacaine for Pain Control During and After Lower Abdominal Surgery. (2015)

Compared to bupivacaine, epidural clonidine provided effective intraoperative and postoperative analgesia in selected patients, resulting in a decreased intravenous pain medication requirement and prolonged duration of analgesia after epidural infusion was discontinued.
Increased Serum Concentrations of Soluble Vascular Cell Adhesion Molecule-1 in Uterine Cervical Cancer (1998)

The purpose of this investigation was to determine the serum concentration of soluble vascular cell adhesion molecule-1 (sVCAM-1) in women with squamous cell carcinoma of the cervix. Serum samples were obtained from 38 Japanese women with squamous cell carcinoma of the cervix before initial treatment (12 in stage 0, 7 in stage I, 5 in stage II, 9 in stage III and 5 in stage IV), 7 patients with a recurrence of this cancer, and 18 healthy female volunteers. Serum sVCAM-1 was measured with a sandwich enzyme-linked immunosorbent assay. Serum concentrations (means+/-SD) of sVCAM-1 in the healthy volunteers, the patients with cervical cancer in stages 0-IV and in the patients with recurrent tumors were 597.2+/-151.4, 690.1+/-214.2, 1,234.8+/-466.1, 1,159.8+/-825.8, 1,529.6+/-662.0, 1,053.0+/-228.8, and 1,134.8+/-211.3 ng/ml, respectively. Values for patients with stages I-IV or recurrent cervical cancer were significantly increased compared to those for healthy volunteers (p < 0.05). Results suggest that sVCAM-1 is shed from endothelial cells in the cancerous tissue.
NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides. (1995)

A procedure for the enzymatic synthesis of uniformly 13C15N-labeled DNA oligonucleotides in milligram quantities for NMR studies is described. Deoxynucleotides obtained from microorganisms grown on 13C and 15N nutrient sources are enzymatically phosphorylated to dNTPs, and the dNTPs are incorporated into oligonucleotides using a 3'-5' exonuclease-deficient mutant of Klenow fragment of DNA polymerase I and an oligonucleotide template primer designed for efficient separation of labeled product DNA from unlabeled template. The labeling strategy has been used to uniformly label one or the other oligonucleotide strand in the DNA duplex dGGCAAAACGG.dCCGTTTTGCC in order to facilitate assignment and structure determination by NMR. Application of 15N and 13C heteronuclear NMR experiments to isotopically labeled DNA is presented.
Amylin deposition in the brain: A second amyloid in Alzheimer disease? (2013)

Metabolic disorders and aging promote accumulation of amylin amyloid in the cerebrovascular system and gray matter, altering microvasculature and tissue structure. Amylin amyloid formation in the wall of cerebral blood vessels may also induce failure of elimination of Aβ from the brain, thus contributing to the etiology of AD.
Life cycle and pathogenesis of hepatitis D virus: A review (2013)

Hepatitis D virus (HDV) is a defective RNA virus which requires the help of hepatitis B virus (HBV) virus for its replication and assembly of new virions. HDV genome contains only one actively transcribed open reading frame which encodes for two isoforms of hepatitis delta antigen. Post-translational modifications of small and large delta antigens (S-HDAg and L-HDAg) involving phosphorylation and isoprenylation respectively confer these antigens their specific properties. S-HDAg is required for the initiation of the viral genome replication, whereas L-HDAg serves as a principal inhibitor of replication and is essential for the assembly of new virion particles. Immune mediation has usually been implicated in HDV-associated liver damage. The pathogenesis of HDV mainly involves interferon-α signaling inhibition, HDV-specific T-lymphocyte activation and cytokine responses, and tumor necrosis factor-alpha and nuclear factor kappa B signaling. Due to limited protein coding capacity, HDV makes use of host cellular proteins to accomplish their life cycle processes, including transcription, replication, post-transcriptional and translational modifications. This intimate host-pathogen interaction significantly alters cell proteome and is associated with an augmented expression of pro-inflammatory, growth and anti-apoptotic factors which explains severe necroinflammation and increased cell survival and an early progression to hepatocellular carcinoma in HDV patients. The understanding of the process of viral replication, HBV-HDV interactions, and etio-pathogenesis of the severe course of HDV infection is helpful in identifying the potential therapeutic targets in the virus life cycle for the prophylaxis and treatment of HDV infection and complications.
Diagnosis of interstitial cystitis/ painful bladder syndrome in patients with overactive bladder symptoms. (2007)

Overactive bladder (OAB) and interstitial cystitis (IC) have similar symptoms, including urinary urgency/frequency and nocturia, making them difficult to differentiate on the basis of clinical presentation alone. Both conditions may represent a clinical manifestation of a hypersensitive bladder and should be included in the differential diagnosis for patients who present with urgency/ frequency. It is especially important that IC be considered in patients with OAB that is refractory to treatment. The proposed diagnostic framework may be useful for differentiating IC from OAB and for facilitating appropriate treatment.
Recruitment Failure in American Avocets and Black-Necked Stilts Nesting at Kesterson Reservoir, California, 1984-1985 (1989)

We evaluated recruitment by American Avocets (Recurvirostra americana) and Black-necked Stilts (Himantopus mexicanus) in 1984 and 1985 at the selenium-contaminated Kesterson Reservoir and at a nearby reference site, the Volta Wildlife Area, both in western Merced County, California. Nests were monitored to determine their outcomes, including hatching success, and broods were censused three times per week during the breeding season. Chicks were counted and broods classified according to age. Although large numbers of chicks were presumed to have hatched at Kesterson, only one chick older than about 2 weeks of age was observed there during the 2-year study. It is unlikely that chicks of either species survived to fledging at Kesterson during the study period, whereas one-month-old or older avocet and stilt broods were seen both years at Volta. Selenium occurred at high concentrations in food-chain organisms only at Kesterson and was the most likely cause of the recruitment failure of stilts and avocets at Kesterson Reservoir.
Flashover Tests on Large Air Gaps with DC Voltage and with Switching Surges Superimposed on DC Voltage (1970)

Part I of the paper gives the results of flashover tests carried out on rod-plane and rod-rod gaps of both polarities, with applied dc valtace up to 1600 kV.
Photocatalytic Hydrogen Generation of CuO and WO<sub>3</sub> Co-Loaded TiO<sub>2 </sub>Nanotubes (2011)

TiO 2 nanotube was prepared by anodization method. CuO and WO 3 were loaded by wet impregnation method. The microstructure, phase characteristic and photoelectrocatalytic hydrogen generation performance were studied. The annealed samples were soaked in a mixture solution containing tungstic acid and Cu (NO 3 ) 2 and then post-annealed to fabricate Cu-loaded TiO 2 nanotube. The obtained samples were characterized by SEM, EDX, XRD and the photoelectrocatalytic hydrogen generation under UV illumination was measured and compared.
A Symbolic Representation of the Bankruptcy of Amsterdam in the Year 1763 (1981)

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Energy Efficient RGBW Pixel Configuration for Light-Emitting Displays (2009)

A study on LED displays has been conducted exploring a more efficient method for color generation than the traditional method. The study is comprehensive and thoroughly performed employing various sets of experiments in order to examine the functionality of the new proposed scheme which includes a literature review, theoretical modeling based on a scientific study, experimental data measurements of a developed prototype, and statistical data based on a survey. This study resulted in very interesting outcomes that may lead to a tremendous change in the existing LED display technology.
Targeted Delivery of Magnetic Cobalt Nanoparticles to the Eye Following Systemic Administration (2010)

The eye offers a unique environment in the body to study progression and response to treatment of various ocular, vascular, and neurologic diseases as they occur in vivo. Due to its clear optical media, we can directly view blood vessels and nerve tissue, which often reflect the health of these tissues in the rest of the body. There are limitations to topical, periocular, or intraocular drug delivery that include access of the drug to the posterior segment of the eye and complications such as local scarring, hemorrhage, retinal detachment, cataract formation, or infection. The aim of this proof‐of‐concept study was to determine if systemically delivered magnetic cobalt nanoparticles (Co‐MNP) could be directed to the eye of C57Bl mice via a unidirectional magnetic field. Both radioactive biodistribution studies and confocal imaging confirmed the increased presence of magnetic particles in the eye following magnetic targeting.
Hyaluronan and synovial joint: function, distribution and healing (2013)

Synovial fluid is a viscous solution found in the cavities of synovial joints. The principal role of synovial fluid is to reduce friction between the articular cartilages of synovial joints during movement. The presence of high molar mass hyaluronan (HA) in this fluid gives it the required viscosity for its function as lubricant solution. Inflammation oxidation stress enhances normal degradation of hyaluronan causing several diseases related to joints. This review describes hyaluronan properties and distribution, applications and its function in synovial joints, with short review for using thiol compounds as antioxidants preventing HA degradations under inflammation conditions.
Long Hydrocarbon Chain Keto Diols and Diacids that Favorably Alter Lipid Disorders in Vivo (2004)

Keto-substituted hydrocarbons with 11-19 methylene and bis-terminal hydroxyl and carboxyl groups have been synthesized and evaluated in both in vivo and in vitro assays for their potential to favorably alter lipid disorders including metabolic syndrome. Compounds were assessed for their effects on the de novo incorporation of radiolabeled acetate into lipids in primary cultures of rat hepatocytes as well as for their effects on lipid and glycemic variables in obese female Zucker fatty rats [Crl:(ZUC)-faBR] following 1 and 2 weeks of oral administration. The most active compounds were found to be symmetrical with four to five methylene groups separating the central ketone functionality and the gem dimethyl or methyl/aryl substituents. Furthermore, biological activity was found to be greatest in both in vivo and in vitro assays for the tetramethyl-substituted keto diacids and diols (e.g., 10c, 10g, 14c), and the least active were shown to be the bis(arylmethyl) derivatives (e.g., 10e, 10f, 14f). Compound 14c dose-dependently elevated HDL-cholesterol, reduced triglycerides, and reduced NEFA, with a minimum effective dose of 30 mg/kg/day. Compound 1 g dose-dependently modified non-HDL-cholesterol, triglycerides, and nonesterified fatty acids, with a minimum effective dose of 10 mg/kg/day. At this dose, compound 10g elevated HDL-cholesterol levels 2-3 times higher than pretreatment levels, and a dose-dependent reduction of fasting insulin and glucose levels was observed.
<scp>T</scp>reg <scp>C</scp>ells <scp>A</scp>re <scp>N</scp>egatively <scp>C</scp>orrelated with <scp>I</scp>ncreased <scp>M</scp>emory <scp>B C</scp>ells in <scp>P</scp>re‐eclampsia <scp>W</scp>hile <scp>M</scp>aintaining <scp>S</scp>uppressive <scp>F</scp>unction on <scp>A</scp>utologous <scp>B</scp>‐<scp>C</scp>ell <scp>P</scp>roliferation (2013)

The study indicates that a negative correlation between Tregs and memory B cells is present in women with PE, characterized by a systemic decrease in Tregs and an increase in memory B cells. Although the quantitative deficit of Tregs is present in PE, the Tregs still have a suppressive role in autologous B-cell proliferation.
Modifikasi Permainan Tradisional Presean Tingkat Sekolah Dasar di SD Islam NW Tanah Abror (2019)

Penelitian ini bertujuan untuk menghasilkan modifikasi permainan tradisional peresean tingkat sekolah dasar yang baik, efektif dan menyenangkan yang dapat digunakan oleh guru pendidikan jasmani. Penelitian ini merupakan penelitian pengembangan ( education research and development (R & D) meliputi sebagai berikut: (1) pengembangan produk; (2) pengumpulan informasi; (3) desain produk (draf model); (4) validasi ahli dan revisi; (5) uji coba lapangan skala kecil dan revisi; (6) uji coba lapangan skala besar dan revisi; (7) pembuatan produk final, revisi uji coba skala kecil pada 10 siswa Sekolah Dasar Islam NW Tanah Abror; (8) uji coba produk skala besar pada 20 siswa Sekolah Dasar Islam NW Tanah Abror; (9) revisi produk dan, . Instrumen yaitu: (1) Lembar wawancara dan kuesioner. Teknik analisis data yaitu analisis deskriptif kualitatif dan kuantitatif. Hasil penelitian ini berupa modifikasi permainan tradisional peresean. Hasil menunjukkan bahwa modifikasi permainan olahraga tradisional peresean untuk sekolah dasar di Sekolah Dasar Islam NW Tanah Abror direspon baik dan efektif. Hal ini dibuktikan persentase, nilai rata-rata pada kuesioner ahli adalah 66% pada uji coba sekala kecil, 73% pada skala besar dan respon peserta didik positif sehingga dapat digunakan dalam pembelajaran. DOI: 10.29408/porkes.v2i1.1468
Metaplastic breast carcinoma (1996)

Metaplastic breast carcinoma, a rare entity, accounts for only about 0.02% of all breast carcinomas. It involves the transformation of mammary neoplasms into osteoid and chondroid substances. Because of the relatively small patient population and the limited number of controlled studies, there is confusion regarding its classification and staging. Its histogenesis is unknown. The authors describe a 65-year-old woman with findings consistent with metaplastic breast carcinoma. Theories as to etiology and prognosis as well as treatment are discussed.
CD28: a signalling perspective (1996)

CD28 and the related molecule cytotoxic T lymphocyte-associated molecule-4 (CTLA-4), together with their natural ligands B7.1 and B7.2, have been implicated in the differential regulation of several immune responses. CD28 provides signals during T cell activation which are required for the production of interleukin 2 and other cytokines and chemokines, and it has also been implicated in the regulation of T cell anergy and programmed T cell death. The biochemical signals provided by CD28 are cyclosporin A-resistant and complement those provided by the T cell antigen receptor to allow full activation of T cells. Multiple signalling cascades which may be independent of, or dependent on, protein tyrosine kinase activation have been demonstrated to be activated by CD28, including activation of phospholipase C, p21ran, phosphoinositide 3-kinase, sphingomyelinase/ceramide and 5-lipoxygenase. The relative contributions of these cascades to overall CD28 signalling are still unknown, but probably depend on the state of activation of the T cell and the level of CD28 activation. The importance of these signalling cascades (in particular the phosphoinositide 3-kinase-mediated cascade) to functional indications of CD28 activation, such as interleukin 2 gene regulation, has been investigated using pharmacological and genetic manipulations. These approaches have demonstrated that CD28-activated signalling cascades regulate several transcription factors involved in interleukin 2 transcriptional activation. This review describes in detail the structure and expression of the CD28 and B7 families, the functional outcomes of CD28 ligation and the signalling events that are thought to mediate these functions.
Terminal Functionalization of Polypropylene by Radical-Mediated Thiol−Ene Addition (2005)

The radical-mediated addition of alkanethiols to the terminal unsaturation of polypropylene (PP) is used to prepare functional polyolefin derivatives without altering the molecular weight of the parent material. The sulfide product of thiol−ene addition, as well as the internal olefin resulting from a remarkable thiyl radical-mediated olefin migration, is characterized using a model hydrocarbon, 2,4-dimethylhept-1-ene. The yield of 3-mercaptopropyltrimethoxysilane (MPTMS) addition to atactic-PP is examined in detail, and the functionalization of high molecular weight isotactic-PP is used to produce moisture-curing resins that bind to siliceous fillers. The limited unsaturation content of PP, along with the high temperatures needed to modify the resin in its melt state, is shown to impact negatively on the reaction conversion due to the reversibility of thiyl radical attack on olefin.
Analysis of repetitive sequence elements containing tRNA-like sequences (1985)

Several repetitive sequence elements from diverse species share extensive sequence homology with tRNA molecules. Analysis of the tRNA-like sequences within these elements suggest that they have originated from authentic tRNA sequences. Elements containing tRNA-like sequences can be divided into three distinct groups whose members share extensive sequence homology, have similar sequence organization and have unique species distribution. We suggest that these three groups represent independent examples of retroposon families that have originated from tRNAs.
Carbonation of polymeric organolithium compounds: Effects of chain end structure (1992)

Abstract The carbonation of the adduct of poly(styryl)lithium with 1,1‐diphenylethylene with gaseous carbon dioxide in benzene proceeds in high yield (>99%) compared to the inefficient functionalizations for poly(styryl)lithium (66%), poly(isoprenyl)lithium (59%), and polybutadienyl)lithium (27%). The quantitative carboxylation of poly(isoprenyl)lithium and poly(butadienyl)lithium requires the presence of larger amounts of Lewis bases such as THF ([THF]/[Li] = 300) or TMEDA ([TMEDA]/[Li] = 46) compared to poly(styryl)lithium in which a ratio of [TMEDA]/[Li] = 12 is effective. © 1992 John Wiley & Sons, Inc.
Photoaffinity labeling of the canine renal receptor for parathyroid hormone. (1982)

Studies were undertaken to identify and characterize components of the parathyroid hormone receptor. An analog of the bovine parathyroid hormone(1-34) sequence was derivatized at the 23-tryptophan position with the photoreactive reagent 2-nitro-5-azidophenylsulfenyl chloride. 2-Nitro-5-azidophenylsulfenyl-bovine parathyroid hormone (NAPS-bPTH) analog retained full biological activity with respect to receptor binding and activation of adenylate cyclase in canine renal cortical plasma membranes. 125I-bPTH(1-34) analog was also derivatized without loss of receptor-binding activity. When 125I-NAPS-bPTH(1-34) analog was incubated with canine renal plasma membranes and then photolyzed, at least two 125I-labeled membrane components were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The incorporation of 125I radioactivity into one of these components (Mr congruent to 60,000) was inhibited when incubation and photolysis were performed in the presence of excess, unlabeled bPTH(1-34). Furthermore, photolysis of membranes in the presence of NAPS-bPTH(1-34) analog led to activation of adenylate cyclase which persisted following washing to remove noncovalently bound peptide, suggesting a functional, covalent hormone-receptor complex had been formed. We conclude that the M r congruent to 60,000 membrane component may be a part of the renal receptor for parathyroid hormone.
Functional and prognostic relevance of the −173 polymorphism of the macrophage migration inhibitory factor gene in systemic‐onset juvenile idiopathic arthritis (2003)

Our study shows the functional relevance of the MIF-173 polymorphism and suggests that the MIF-173*C allele is a predictor of poor outcome in systemic-onset JIA.
Quasi-Orthogonal STBC Using Stretched Constellations for Low Detection Complexity (2007)

Space-time block codes (STBC) from orthogonal designs have been proposed by Alamouti and Tarokh-Jafarkhani-Calderbank, achieving full diversity in the order of the number of transmit antennas. However, orthogonal STBC always result in a symbol rate less than one, if complex symbols are transmitted from more than 2 antennas. Jafarkhani, Tirkkonen-Boariu-Hottinen and Papadias-Foschini have suggested to use quasi-orthogonal STBC (QO-STBC), and it has been shown by various authors that these schemes achieve transmission rate 1 and full diversity, if selected symbols are chosen from suitably rotated constellations. The schemes can, however, lead to a high detection complexity, which has been recently addressed through co-ordinate interleaved orthogonal designs (CIOD) and minimum detection complexity (MDC) QO-STBC, though leading to a slight degradation in performance. In this paper, we present a novel QO-STBC scheme that uses stretched, i.e. non-linearly preprocessed symbol constellations, achieving full diversity and a performance comparable to that of CIOD and MDC, while further reducing detection complexity.