gebhart/patrepeval huggingface dataset:
Cloning, Characterization, and Genetic Mapping of the Rat Type 2 Angiotensin II Receptor Gene (1995)

The renin-angiotensin system plays an important role in blood pressure homeostasis, but the contribution of the type 2 angiotensin II receptor (AT2R) is still unclear. The reports that the AT2R gene has been mapped to the X chromosome in human and rat and the previous report of a gene, Bp3, on the X chromosome responsible for an increase in blood pressure have suggested that the rat AT2R gene (Agtr2) could be this gene. To elucidate whether Agtr2 is Bp3, Agtr2 was cloned. A simple sequence repeat in the 3'-flanking region of this gene was identified and used as a genetic marker to map Agtr2 to the X chromosome at 18.1 cM distal to the androgen receptor locus. This map position is outside the confidence interval reported for Bp3, demonstrating that Agtr2 cannot be Bp3. However, these data will enhance the research into the AT2R biology as well as the study of the X chromosome.
Selective Aggregation of L1210 Leukemia Cells by the Polycation Chitosan<xref ref-type="fn" rid="FN2">2</xref><xref ref-type="fn" rid="FN3">3</xref> (1971)

The polycation chitosan, at concentrations ranging from 0.05–0.006 × 10−3 mEq./ml, selectively aggregated L1210 leukemia cells in vitro when compared to normal murine erythrocytes and bone marrow cells, as well as Sarcoma 37 and Ehrlich ascites cells. Poly-L-lysine and polyethylenimine, however, at these concentrations, did not discriminate between the erythrocytes and L1210 cells. Both chitosan and poly-L-lysine (3.2-0.2 × 10−3 mEq./ml) produced dense aggregation, cytoplasmic swelling, and/or cytolysis of the L1210 cells and increased the permeability of these cells to trypan blue. Poly-L-lysine (0.2 × 10−3 mEq./ml) produced similar morphological alterations of the erythrocytes, whereas chitosan, at this concentration, only minimally aggregated the erythrocytes and did not conspicuously alter their morphology. Incubation of the L1210 cells with chitosan or poly-L-lysine partially inhibited the transplantability of the cells, as measured by survival time, after inoculation into (C57BL × DBA)F1 (BDF1) mice. Immunosuppression with 180 m3/kg cyclophosphamide did not significantly alter this effect. Further, chronic chitosan treatment of BDF1 mice, bearing intraperitoneal implants of the L1210 ascites tumor, retarded the dissemination of the leukemic cells into the peripheral blood.
Biosynthesis of riboflavin in Bacillus subtilis: function and genetic control of the riboflavin synthase complex (1978)

Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729--732, in T. P. Singer (ed.), Flavins and Flavoproteins, Elsevier, Amsterdam, 1976). The formation of alpha and beta subunits is coordinately controlled. Mutants apparently deficient in beta subunits were isolated as riboflavin requires after mutagenesis of B. subtilis with ICR 191. The mutants could grow with diacetyl instead of riboflavin. Growth with diacetyl was associated with the accumulation of substantial amounts of the riboflavin precursor, 6,7-dimethyl-8-(D-ribityl)lumazine. It follows that the mutants are deficient in an enzyme activity required for the formation of the lumazine from the pyrimidine precursor. We conclude that heavy riboflavin synthase is a bifunctional enzyme. The riboflavin synthase activity is mediated by the alpha subunits, whereas the beta subunits are necessary for an earlier biosynthetic step.
Best site for embryo transfer: the upper or lower half of endometrial cavity? (2004)

The implantation or pregnancy rates were similar whether the embryos were deposited in the upper or lower half of the endometrial cavity.
Analysis of factoid questions for effective relation extraction (2005)

We present an analysis of the structured relationships observed in a randomly sampled set of question-like queries submitted to a search engine for a popular online encyclopedic document collection. Our study shows that a relatively small number of binary relationships account for most of the queries in the sample. This empirically validates an approach of analyzing query logs to identify the relationships most relevant to user needs and populating corresponding fact tables from the collection for factoid question answering. Our analysis shows that such an approach can lead to substantial coverage of user questions.
Expression of vascular endothelial growth factor protein in human renal cell carcinoma (2004)

VEGF protein was present in most RCC cells. There was no difference in VEGF expression among the different RCC types. The correlation between VEGF expression and tumour stage and with prognosis indicates the significance of VEGF within tumour growth and progression in RCC.
Using Prediction to Accelerate Coherence (1998)

Most large shared-memo? multiprocessors use directov protocols to keep per-processor caches coherent. Some memory references in such systems, however; suffer long latencies for misses to remotelycached blocks. To ameliorate this latency, researchers have augmented standard coherence protocols with optimizations for specific sharing patterns, such as read-mod@write, producer-consumer. and migratory sharing. This paper seeks to replace these directed solutions with general prediction logic that monitors coherence activity and triggers appropriate coherence actions. This paper takes the first step toward using general prediction IO accelerate coherence protocols by developing and evaluuting the Cosmos coherence message predictor: Cosmos predicts the source and type of the next coherence message for a cache block using logic that is an extension of Yeh and Patt’s two-level PAp branch predictor. For jive scientific applications running on 16 processors, Cosmos has prediction accuracies of 62% to 93%. Cosmos’ high prediction accuracy is a result of predictable coherence message signatures that arise from stable sharing patterns of cache blocks.
Transurethral Evaporation of the Prostate for Treatment of Benign Prostatic Hyperplasia: Results in 168 Patients With up to 12 Months of Followup (1997)

From these results transurethral evaporation of the prostate appears to be safe and effective for treatment of BPH at 12 months of followup.
Detecting deficits of sustained visual attention in delirium (2010)

Patients with delirium showed marked deficits in sustained visual attention, as measured by objective neuropsychological testing. These attentional deficits were mainly mild or absent in patients with dementia and in cognitively normal controls. Objective testing of sustained visual attention has promising utility in detecting delirium, and in discriminating delirium from dementia.
High expression of agrin in hepatocellular and cholangiocellular carcinoma (2005)

Introduction/Aim: The synthesis of proteoglycans, as one of the major components of ECM, was shown to be changed during neoplastic transformation both in tumors and surrounding tissues. Agrin, being a heparan sulfate proteoglycan constituent of the basement membrane, has been implicated in the regulation of cell growth, differentiation, adhesion and motility. The expression of agrin has already been studied in various organs, however, few reports are available about its involvement in carcinogenesis.
Laser heating of thick layers through the backwards, self-sustained propagation of a steep and steady state thermal front (2008)

We investigate theoretically a mode of heating layers using a laser beam. In this mode thermal energy, the temperature of the layer, propagates in a steady-state self sustained fashion from the bottom of the layer towards the surface and may exhibit a very steep front. The propagation exhibits a constant speed, related to the intensity of the power flux. To achieve this heating mode the absorption coefficient has to remain low in low temperatures and increase rapidly as a function of temperature in higher temperatures. Additionally, a significant temperature increase must be generated to trigger this propagation mode, for example through the presence of a strongly absorbing layer beneath the transparent layer. The mode is well suited to semiconductors, especially silicon. A few examples of applications are proposed.
Amino acid transporters: Emerging roles in drug delivery for tumor-targeting therapy (2020)

Amino acid transporters, which play a vital role in transporting amino acids for the biosynthesis of mammalian cells, are highly expressed in types of tumors. Increasing studies have shown the feasibility of amino acid transporters as a component of tumor-targeting therapy. In this review, we focus on tumor-related amino acid transporters and their potential use in tumor-targeting therapy. Firstly, the expression characteristics of amino acid transporters in cancer and their relationship with tumor growth are reviewed. Secondly, the recognition requirements are discussed, focusing on the “acid-base” properties, conformational isomerism and structural analogues. Finally, recent developments in amino acid transporter-targeting drug delivery strategies are highlighted, including prodrugs and nanocarriers, with special attention to the latest findings of molecular mechanisms and targeting efficiency of transporter-mediated endocytosis. We aim to offer related clues that might lead to valuable tumor-targeting strategies by the utilization of amino acid transporters.
Searchable encryption with decryption in the standard model. (2008)

A searchable public key encryption (PEKS) scheme allows to generate, for any given message W , a trapdoor TW , such that TW allows to check whether a given ciphertext is an encryption ofW or not. Of course, TW should not reveal any additional information about the plaintext. PEKS schemes have interesting applications: for instance, consider an email gateway that wants to prioritize or filter encrypted emails based on keywords contained in the message text. The email recipient can then enable the gateway to do so by releasing the trapdoors for the corresponding keywords. This way, the gateway can check emails for these keywords, but it learns nothing more about the email contents. PEKS schemes have first been formalized and constructed by Boneh et al.. But with one exception, no known construction of a PEKS scheme supports the decryption of ciphertexts. That is, known constructions allow to test for a certain message, but they do not allow to retrieve the message, even when having the full secret key. Besides being somewhat unnatural for an encryption scheme, this “no-decryption”-property also limits the applicability of a PEKS scheme. The one exception, a PEKS scheme with decryption due to Fuhr and Paillier, is formulated in the random oracle model, and inherently relies on the statistical properties of the random oracle. In fact, Fuhr and Paillier leave it as an open problem to construct a PEKS scheme with decryption in the standard model. In this paper, we construct the first PEKS scheme with decryption (PEKSD scheme) in the standard model. Our sole assumption is an anonymous IBE scheme. We explain the technical difficulties that arise with previous attempts to build a PEKS scheme with decryption and how we overcome these difficulties. Technically, we isolate a vital additional property of IBE schemes (a property we call well-addressedness and which states that a ciphertext is tied to an identity and will be rejected when trying to decrypt with respect to any other identity) and show how to generically achieve it. Our construction of a PEKSD scheme from an anonymous IBE scheme provides a natural example of a non-shielding construction (in which the decryption algorithm queries the encryption algorithm). Gertner et al. have shown that an IND-CCA secure public key encryption scheme cannot be constructed and proven from an IND-CPA secure scheme in a black-box and shielding way. However, our results give evidence that encryption queries in the decryption algorithm may well prove useful in a security reduction. ∗CWI, Amsterdam, The Netherlands. E-mail: Dennis.Hofheinz@cwi.nl †CWI, Amsterdam, The Netherlands. E-mail: e.n.weinreb@cwi.nl
Austrian consensus guidelines on the management and treatment of portal hypertension (Billroth III) (2017)

The Billroth III guidelines were developed during a consensus meeting of the Austrian Society of Gastroenterology and Hepatology (ÖGGH) and the Austrian Society of Interventional Radiology (ÖGIR) held on 18 February 2017 in Vienna. Based on international guidelines and considering recent landmark studies, the Billroth III recommendations aim to help physicians in guiding diagnostic and therapeutic strategies in patients with portal hypertension.
Development of a Method for Determination of Buckwheat Allergens Using Liquid Chromatography with Tandem Mass Spectrometry (2017)

An analysis technique using LC with tandem MS (MS/MS) has been developed for the determination of buckwheat proteins, including major allergens. A protein solution extracted from buckwheat was reduced, alkylated, and digested by trypsin. Peptide spectra were obtained using full-scan LC-MS/MS analysis, and peptide sequences were determined through a protein search. Nine peptides of the 13S globulin seed storage protein and one peptide of a 16 kDa allergen were selected as the marker peptides, and multiple reaction monitoring conditions were optimized. Using the conditions, different kinds of buckwheat noodles, powders, and other food ingredients were analyzed. As a result, buckwheat samples present all the fragment peaks, whereas other foods, including <em>Sesamum indicum</em>, wheat, and soybeans, are not detected at all. These findings indicate that LC-MS/MS analysis may be applied to the detection of buckwheat food allergens.
Comparison of kinematic and kinetic parameters calculated using a cluster-based model and Vicon’s plug-in gait (2014)

Gait analysis is an important clinical tool. A variety of models are used for gait analysis, each yielding different results. Errors in model outputs can occur due to inaccurate marker placement and skin motion artefacts, which may be reduced using a cluster-based model. We aimed to compare a custom-made cluster model (ClusBB) with Vicon's plug-in gait. A total of 21 healthy subjects wore marker sets for the ClusBB and plug-in gait models simultaneously while walking on a 6-m walkway. Marker and force plate data were captured synchronously and joint angles/moments were calculated using both models. There was good correlation between the models (coefficient of multiple correlations > 0.65) and good intra-session correlation for both models (coefficient of multiple correlations > 0.80). Inter-subject variability was high, ranging from 15° to 40° in the sagittal plane and 11° to 52° in the coronal and transverse planes. Intra-subject variability was small for both ClusBB and plug-in gait models. Inter-subject variance tended to be high in both models for knee abduction/adduction, but particularly so for plug-in gait, suggesting that a cluster-based model may reduce the variability. The inter-subject variance in out-of-sagittal plane data is of particular importance clinically, given the reliance on these datasets in clinical decision-making.
Rapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N‐desmethyl Clozapine (Norclozapine) in human serum (2009)

Clozapine is indicated for the treatment of schizophrenia and related psychotic disorders. Several methods have been developed for monitoring Clozapine levels; however, they possess limited specificity and are often laborious. This study describes a simple liquid chromatography/tandem mass spectrometer (LCMS) method in human serum. The ion transitions monitored were m/z 327, 270, 296 for Clozapine, m/z 313, 192, 227 for Norclozapine and m/z 328, 271 for Loxapine. The assay is linear (25-1000 ng/ml) and showed a good correlation (r=0.98) within the analytical range of 79-1210 ng/ml in human serum. This assay is highly specific and sensitive for the simultaneous measurements of Clozapine and Norclozapine. The simplification of this assay makes it ideal for high throughput analyses of the patient samples in a routine clinical laboratory staffed with general medical technologists.
Oxidative versus Non-oxidative Decarboxylation of Amino Acids: Conditions for the Preferential Formation of Either Strecker Aldehydes or Amines in Amino Acid/Lipid-Derived Reactive Carbonyl Model Systems (2015)

Comparative formation of both 2-phenylethylamine and phenylacetaldehyde as a consequence of phenylalanine degradation by carbonyl compounds was studied in an attempt to understand if the amine/aldehyde ratio can be changed as a function of reaction conditions. The assayed carbonyl compounds were selected because of the presence in the chain of both electron-donating and electron-withdrawing groups and included alkenals, alkadienals, epoxyalkenals, oxoalkenals, and hydroxyalkenals as well as lipid hydroperoxides. The obtained results showed that the 2-phenylethylamine/phenylacetaldehyde ratio depended upon both the carbonyls and the reaction conditions. Thus, it can be increased using electron-donating groups in the chain of the carbonyl compound, small amounts of carbonyl compound, low oxygen content, increasing the pH, or increasing the temperature at pH 6. Opposed conditions (use of electron-withdrawing groups in the chain of the carbonyl compound, large amounts of carbonyl compound, high oxygen contents, low pH values, and increasing temperatures at low pH values) would decrease the 2-phenylethylamine/phenylacetaldehyde ratio, and the formation of aldehydes over amines in amino acid degradations would be favored.
Adhesion reduction in the rabbit uterine horn model using an absorbable barrier, TC-7. (1987)

Use of a resorbable barrier (TC-7) to reduce adhesion formation was examined in the rabbit uterine horn model. Early evaluation (four days) established that the material remained where initially placed. Examination at two, four and eight weeks postoperatively in both control and treated groups revealed that the extent of adhesion formation did not change as a function of time. When compared to control animals without TC-7, the treated rabbits has a significant reduction (P less than .01) in postoperative adhesion formation. No complications from use of this fabric were noted, and no gross remnants were noted at the two-week time point. TC-7 appears to be an efficacious barrier for reducing adhesion formation in the rabbit uterine horn model.
Antimicrobial Resistance (2007)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Pleiotropic mutations in <i>Saccharomyces cerevisiae</i> affecting sterol uptake and metabolism (1988)

Sterol uptake control mutants (upc-) have been isolated via ethylmethanesulfonate mutagenesis from wild-type Saccharomyces cerevisiae. These mutants are heme and sterol competent but possess the ability to accumulate exogenous sterol(s) under aerobic conditions. Previous studies demonstrate sterol uptake only in a hem-, erg- background; however, the Upc- strains described here are Hem+ and do not require exogenous sterol for growth. We were unable to obtain viable hem+, erg-, upc+ recombinants; such combinations appear to be lethal. Isolates of Upc mutants demonstrated different levels of sterol uptake, and sterol analysis revealed a broad phenotypic range with regard to amounts and accumulation of ergosterol and non-ergosterol sterols. Assays of acyl CoA: ergosterol acyltransferase and sterol ester hydrolase showed no apparent difference in activity between Upc mutants and the wild type.
CTCFBSDB: a CTCF-binding site database for characterization of vertebrate genomic insulators (2007)

Recent studies on transcriptional control of gene expression have pinpointed the importance of long-range interactions and three-dimensional organization of chromatins within the nucleus. Distal regulatory elements such as enhancers may activate transcription over long distances; hence, their action must be restricted within appropriate boundaries to prevent illegitimate activation of non-target genes. Insulators are DNA elements with enhancer-blocking and/or chromatin-bordering functions. In vertebrates, the versatile transcription regulator CCCTC-binding factor (CTCF) is the only identified trans-acting factor that confers enhancer-blocking insulator activity. CTCF-binding sites were found to be commonly distributed along the vertebrate genomes. We have constructed a CTCF-binding site database (CTCFBSDB) to characterize experimentally identified and computationally predicted CTCF-binding sties. Biological knowledge and data from multiple resources have been integrated into the database, including sequence data, genetic polymorphisms, function annotations, histone methylation profiles, gene expression profiles and comparative genomic information. A web-based user interface was implemented for data retrieval, analysis and visualization. In silico prediction of CTCF-binding motifs is provided to facilitate the identification of candidate insulators in the query sequences submitted by users. The database can be accessed at http://insulatordb.utmem.edu/