gebhart/patrepeval huggingface dataset:
Cyclins and CDKS in development and cancer: lessons from genetically modified mice (2006)

From yeast to humans, cell cycle progression and cell division are driven by the sequential activation of a group of serine-threonine kinases called cyclin-dependent kinases (Cdks). Multiple Cdks control the cell cycle in mammals and have been long considered essential for normal proliferation, development and homeostasis. The importance of the Cdk-cyclin complexes in cell proliferation is underscored by the finding that deregulation of the Cdk activity is found in virtually the whole spectrum of human tumors. Recent information from gene-targeted mouse models for the various cyclins and Cdks have made some of the generally accepted concepts of cell cycle regulation to be revised and new and exciting questions to be investigated. Unexpectedly, most of the canonical Cdk-cyclin complexes have turned out to be dispensable for cell proliferation due to a high level of functional redundancy, promiscuity and compensatory mechanisms. As a consequence, a "yeast-like" model where only one Cdk is essential to drive all stages of cell cycle progression is starting to be envisioned for mammalian cells. Moreover, the specific molecular players that drive the cell cycle in mammals seem to be cell-type-specific, and new, non-canonical functions of cyclins and Cdks have been revealed. This review will discuss these new findings and their implications for cancer therapy.
Evidence for a Structural Role for Acid-Fast Lipids in Oocyst Walls of <i>Cryptosporidium</i> , <i>Toxoplasma</i> , and <i>Eimeria</i> (2013)

Oocysts, which are essential for the fecal-oral spread of coccidia, have a wall that is thought responsible for their survival in the environment and for their transit through the stomach and small intestine. While oocyst walls of Toxoplasma and Eimeria are strengthened by a porous scaffold of fibrils of β-1,3-glucan and by proteins cross-linked by dityrosines, both are absent from walls of Cryptosporidium. We show here that all oocyst walls are acid fast, have a rigid bilayer, dissolve in organic solvents, and contain a complex set of triglycerides rich in polyhydroxy and long fatty acyl chains that might be synthesized by an abundant polyketide synthase. These results suggest the possibility that coccidia build a waxy coat of acid-fast lipids in the oocyst wall that makes them resistant to environmental stress.
Tannic acid is an inhibitor of CXCL12 (SDF-1alpha)/CXCR4 with antiangiogenic activity. (2003)

These studies indicate that tannic acid is a novel selective CXCL12/CXCR4 antagonist and consequently may provide a mechanistic basis for the reported antitumor and anti-inflammatory properties of tannic acid.
Inhibition of human ovarian cancer cell growth in vitro and in nude mice by prostaglandin D2. (1986)

In vitro and in vivo effects of prostaglandin D2 on human ovarian tumor growth were examined by using a cell line, designated HR, derived from ascites of a patient with serous cystadenocarcinoma of the ovary. The HR cell proliferation in vitro was dose dependently inhibited between concentrations of 0.1 and 4.0 micrograms of prostaglandin D2 per ml. From results of 51Cr release assays and trypan blue dye exclusion tests the inhibitory effect seemed to result from a direct cytotoxic effect by prostaglandin D2. All DNA, RNA, and protein syntheses by the HR cells were also inhibited in a dose-dependent manner with exposure time of 48 h to prostaglandin D2. When 5 X 10(5) HR cells were inoculated to nude mice, the 50% survival time of them in untreated groups was 52 days after inoculation. Although 4 mg of prostaglandin D2 per kg caused inhibition of the tumor growth, a significant prolongation of the survival time was not observed. On the other hand, the 50% survival time of nude mice treated with 12 mg of prostaglandin D2 per kg was significantly (P less than 0.05) prolonged to 67 days, in addition to a significant inhibition of the tumor growth.
Portable information filtering system: network information servers (2002)

A communications link can only be considered successful when the desired information is delivered to a user of the communications service. In these days of overwhelming digital information broadcast via numerous wide-area networks including the Internet and corporate intranets, the ability of an individual user to obtain relevant, needed information is becoming increasingly difficult. Information filtering systems perform a data extraction function, analyzing an incoming stream of information and selecting information that matches the needs of its users. Inclusion of an information filtering mechanism in the communications system ensures that the desired information is delivered to a user. Currently, information filtering systems are based on proprietary implementations that are not easily moved to other platforms, do not readily scale for larger volumes of information, and cannot be easily modified to handle new forms of data. This paper presents an overview of the work we have done using the relational database model to implement an information filtering system. We describe the prototype information filtering system we have developed and its initial system accuracy and performance measurements. Use of the relational model provides the information filter system with the critical features of portability, scalability, and flexibility.
Effect of Particle Size of Filler on Properties of Nylon-6 (2004)

Particulate reinforced thermoplastic composites are designed to improve the properties and to lower the overall cost of engineering plastics. In this study the effects of adding mica with variable particle size on the mechanical, thermal, electrical and rheological properties of nylon-6 was investigated. Composites of nylon-6 with v arying concentrations (viz. 5 to 40 weights %) of mica were prepared by twin screw extrusion. The composite showed improved mechanical, thermal as well electrical properties on addition of filler. It is also observed that mechanical properties, electrical properties as well as thermal properties increases with decrease in particle size.
560. GNE Gene Replacement in Hereditary Inclusion Body Myopathy (2006)

Hereditary inclusion body myopathy (HIBM) is an adult onset neuromuscular disorder currently without treatment options. The disease has been recently found to be associated with mutations in the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) gene, which is the rate limiting bi-functional enzyme that catalyzes the first two steps of sialic acid biosynthesis (Huizing, Hermos et al. 2002). Sialic acid (Sia) is the negatively charged terminal carbohydrate on a variety of N-linked and O-linked glycoproteins, and mediates numerous cell-cell and protein-protein interactions. Reduced GNE activity in HIBM impairs Sia production, consequently leading to decreased sialylation of a variety of glycoproteins, including a critical muscle protein, alpha-dystroglycan (α-DG). Altered glycosylation levels observed in HIBM are likely associated with less active membrane potential, and hypo-excitable myofilament function, causing hyporeflexia early in disease course. Preliminary studies demonstrate that replacement of the GNE gene in a GNE gene mutated cell line (Lec3) rescues UDP-GlcNAc 2-epimerase activity and cell surface polysialic acid (PSA) activity. Our hypothesis is that replacement of GNE function through liposomal particle-GNE gene delivery may result in increased myofiber Sia production and subsequent improved muscle function. We are using both the normal and sialuria forms of GNE cDNA togenerate expression vectors. These liposomal particle vectors will be tested in the Lec3 and other cell lines to demonstrate production of GNE mRNA and protein and restoration of GNE enzymatic function. Data will be shown demonstrating the development of expression vectors and their function in cell lines. In addition, mouse GNE:p.M712T knock in mice (GNE null mice do not develop beyond embryonic day 7–10) have been developed to test the GNE vectors physiological effects. We are next studying the effects of different delivery routes, dosages, physiological responses and the duration of effects in these mouse mutants. Our intention is to develop a gene therapy GNE vehicle, which can be repeatedly administered to patients (via compassionate IND) without provoking nullifying immune responses while achieving therapeutically significant numbers of transfected cells. Updated results will be presented.
Construction and use of a nontoxigenic strain of Pseudomonas aeruginosa for the production of recombinant exotoxin A (1995)

To express recombinant forms of Pseudomonas aeruginosa exotoxin A in high yield, we have developed a nontoxigenic strain of P. aeruginosa derived from the hypertoxigenic strain PA103. The nontoxigenic strain, designated PA103A, was produced by the excision marker rescue technique to replace the toxA structural gene in PA103 with an insertionally inactivated toxA gene. The PA103A strain (ToxA-) was used subsequently as the host strain for the expression and production of several recombinant versions of exotoxin A, and the results were compared with exotoxin A production in other P. aeruginosa and Escherichia coli strains. Use of the PA103A strain transformed with the high-copy-number pRO1614 plasmid bearing various toxA alleles resulted in final purification yields of exotoxin A averaging 23 mg/liter of culture. By comparison, exotoxin A production in other expression systems and host strains yields approximately 1/4 to 1/10 as much toxin.
Perceptually optimized gain function for cochlear implant signal-to-noise ratio based noise reduction (2012)

Noise reduction in cochlear implants has achieved significant speech perception improvements through spectral subtraction and signal-to-noise ratio based noise reduction techniques. Current methods use gain functions derived through mathematical optimization or motivated by normal listening psychoacoustic experiments. Although these gain functions have been able to improve speech perception, recent studies have indicated that they are not optimal for cochlear implant noise reduction. This study systematically investigates cochlear implant recipients' speech perception and listening preference of noise reduction with a range of gain functions. Results suggest an advantageous gain function and show that gain functions currently used for noise reduction are not optimal for cochlear implant recipients. Using the cochlear implant optimised gain function, a 27% improvement over the current advanced combination encoder (ACE) stimulation strategy in speech weighted noise and a 7% improvement over current noise reduction strategies were observed in babble noise conditions. The optimized gain function was also most preferred by cochlear implant recipients. The CI specific gain function derived from this study can be easily incorporated into existing noise reduction strategies, to further improve listening performance for CI recipients in challenging environments.
Chemoselective Zinc/HCl Reduction of Halogenated β-Nitrostyrenes: Synthesis of Halogenated Dopamine Analogues (2014)

A detailed account regarding the synthesis of 2- and 5-halogenated dopamine is given. The key step is a chemoselective reduction of a nitrostyrene by Zn/HCl at 0 °C. These conditions represent a simple, low-cost alternative to reduction by water-sensitive hydride donors and two-step procedures. Under these conditions, aryl fluoride, chloride, and bromide groups are stable. However, ­iodine undergoes significant reductive dehalogenation.
An investigation of GaAs films grown by MBE at low substrate temperatures and growth rates (1983)

We have achieved the first successful growth of GaAs films with good electrical properties at substrate temperatures below 480 °C. The electrical and photoluminescence properties of films grown at Ts≊380 and 450 °C have been dramatically improved by reducing the growth rate significantly below the usual rate of ∼1 μm/h. Reductions in the growth rate can be expected to improve the quality of MBE films by allowing sufficient time for Ga and As adatoms to jump to equilibrium lattice positions before they are incorporated into the growing crystal by interacting with other adatoms. We further observed that the density of a major morphological defect of MBE GaAs films—the oval defect—was strongly correlated with the growth rate. The density of such defects was ∼500 cm−2 for films grown at ∼0.02 μm/h.
Industrial Brazing Practice (2003)

Written by an expert with over forty years of experience, this book covers the six rules of brazing and how they impact the various procedures that are an essential part of brazing technology. Exploring what is and what is not good brazing practice, the author presents readers with a reference that will help them deal with the problems they face in
Interaction of β-Catenin and TIF2/GRIP1 in Transcriptional Activation by the Androgen Receptor (2005)

The multifunctional oncoprotein beta-catenin interacts with the activation function-2 domain of androgen receptor (AR) to stimulate androgen receptor transcriptional activity, increase sensitivity, and broaden specificity of ligand interactions. beta-Catenin interacts with androgen receptor in close proximity to the binding groove for P160 coactivators such as transcriptional intermediary factor-2 (TIF2)/glucocorticoid receptor interacting protein-1 (GRIP1). beta-Catenin can also bind directly to TIF2/GRIP1. Both N- and C-terminal regions of beta-catenin are needed for optimal interaction with TIF2/GRIP1. We show that distinct residues of beta-catenin are responsible for both binding and functional interactions with androgen receptor and with TCF4, thus allowing the introduction of missense mutations that selectively affect these interactions. beta-Catenin and TIF2/GRIP1 are each able to mediate binding between the other and androgen receptor in functional interactions that enhance ligand-dependent transcription. The data strongly imply that AR, beta-catenin, and TIF2/GRIP1 bind in a three-way interaction that mediates transcription. Lastly, we observed that a beta-catenin C-terminal peptide containing 229 amino acids can bind TIF2/GRIP1 and AR but has a profound dominant inhibitory effect on ligand-dependent transcription. We propose that beta-catenin may play an integral role in formation of the androgen-receptor transcriptional complex.
Grafted Crystalline Poly‐Perfluoroacrylate Structures for Superhydrophobic and Oleophobic Functional Coatings (2012)

This report describes the preparation of superhydrophobic and oleophobic surfaces by grafting of poly(perfluorodecylacrylate) chains with initiated chemical vapor deposition on silicon substrates. The grafting enhances the formation of a semicrystalline phase. The crystalline structures reduce the polymer chain mobility, resulting in nonwetting surfaces with both water and mineral oil. On the contrary, the same contacting liquid easily wets the amorphous ungrafted polymer.
Silicon Carbide Ultraviolet Photodetector Modeling, Design and Experiments (2012)

We report measurements and modeling of silicon carbide (SiC) based ultraviolet photodetectors for the detection of light in the mid-to-short ultraviolet range where SiC’s absorption coefficients are high and the corresponding penetration depths are low. These large absorption coefficients result in increased susceptibility of photo-generated electron and holes to surface recombination and therefore give rise to lower quantum efficiencies. To increase responsivity and extend the detection capability of these photodetectors to short ultraviolet wavelengths (or UVC), we measure an existing silicon carbide avalanche photodiode (APD) designed and fabricated for 280 nm operation by General Electric Global Research Center, and then develop models and techniques to increase their operation range to lower UV wavelengths. The measurements aid the development and calibration of a silicon carbide modeling and design suite that is currently being used to assist the design of a new silicon carbide APD for UVC detection. Here the design considerations require low operating voltages, low noise, low dark count rate and high responsivity. We plan to satisfy design criteria by engineering thickness and doping of stacked layers as well as by designing an APD surface that gives rise to minimal recombination of electrons and holes generated by the incident light.
Metalloboranes. II. Transition metal complexes of B10H122- (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMetalloboranes. II. Transition metal complexes of B10H122-Frank Klanberg, P. A. Wegner, G. W. Parshall, and E. L. MuettertiesCite this: Inorg. Chem. 1968, 7, 10, 2072–2077Publication Date (Print):October 1, 1968Publication History Published online1 May 2002Published inissue 1 October 1968https://pubs.acs.org/doi/10.1021/ic50068a025https://doi.org/10.1021/ic50068a025research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Transcription Factor Gene <i>AP-2</i>γ Essential for Early Murine Development (2002)

Transcription factor gene AP-2 gamma belongs to a family of four closely related genes. AP-2 gamma had been implicated in multiple functions during proliferation and differentiation based on its expression pattern in trophoblast, neural crest, and ectoderm cells in murine embryos. In order to address the question of the role of AP-2 gamma during mammalian development, we generated mice harboring a disrupted AP-2 gamma allele. AP-2 gamma heterozygous mice are viable and display reduced body sizes at birth but are fertile. Mice deficient for AP-2 gamma, however, are growth retarded and die at days 7 to 9 of embryonic development. Immunohistochemical analysis revealed that the trophectodermal cells that are found to express AP-2 gamma fail to proliferate, leading to failure of labyrinth layer formation. As a consequence, the developing embryo suffers from malnutrition and dies. Analysis of embryo cultures suggests that AP-2 gamma is also implicated in the regulation of the adenosine deaminase (ADA) gene, a gene involved in purine metabolism found expressed at the maternal-fetal interface. Therefore, AP-2 gamma seems to be required in early embryonic development because it regulates the genetic programs controlling proliferation and differentiation of extraembryonic trophectodermal cells.
Quality of security service (2001)

Abstract : We examine the concept of security as a dimension of Quality of Service in distributed systems. Implicit to the concept of Quality of Service is the notion of choice or variation. Security services also offer a range of choice both from the user perspective and among the underlying resources. We provide a discussion and examples of user-specified security variables and show how the range of service levels associated with these variables can support the provision of Quality of Security Service, whereby security is a constructive network management tool rather than a performance obstacle. We also discuss various design implications regarding security ranges provided in a QoS-aware distributed system.
Androgen receptor expression and DNA content of paraffin‐embedded archival human prostate tumors (2002)

Flow cytometric analysis of archival prostate tumor can be used for rapid determination of aneuploid DNA content and AR expression in subpopulations of nuclei isolated from formalin-fixed/paraffin-embedded prostate tumor blocks.
LINGUAGEM: MÚSICA, ARTE, CULTURA E INFLUÊNCIA NO EMI, CAMPUS CHAPECÓ (2012)

Este trabalho apresenta uma pesquisa com a finalidade de analisar de forma cientifica a musica como forma de linguagem e expressao artistica e a influencia que ela exerce nos alunos do Ensino Medio Integrado,IF-SC Campus Chapeco.
Pioglitazone increases secretion of high-molecular-weight adiponectin from adipocytes (2006)

Adiponectin is an adipocyte-derived serum protein that plays important roles in energy homeostasis, obesity, and insulin sensitivity. Using sucrose gradients and Western blotting of nondenaturing gels, we examined the adiponectin isoforms secreted from human adipose tissue, human and mouse adipocytes, and cell lines in response to pioglitazone added in vitro. The predominant form secreted from adipose tissue in vitro was the high-molecular-weight (HMW) isoform, with small amounts of low-molecular-weight (LMW) forms present. The addition of pioglitazone (1-3 micromM) in vitro increased the secretion of the HMW isoform, with no significant effect on the other isoforms. Human adipose tissue was also examined for changes in adiponectin mRNA levels upon pioglitazone treatment. No difference was detected, suggesting that the effect of pioglitazone is not at the transcriptional level but, rather, at a posttranscriptional phase of the secretory pathway. Additional experiments were conducted to determine whether adiponectin expression was mechanistically similar in other adipose cells. Examination of primary human adipocytes revealed an increase in intracellular HMW isoform with a decline in LMW forms following pioglitazone treatment, with a corresponding increase in the secreted HMW form. Similar results were observed with primary mouse adipocytes, 3T3-F422A cells, and SGBS human adipocyte cells, although differences in the distribution of HMW and LMW isoforms were apparent between cell types. Although there are differences in isoforms between species, in all cases pioglitazone served to increase the secretion of the HMW form of adiponectin.
Look Wider to Match Image Patches With Convolutional Neural Networks (2016)

When a human matches two images, the viewer has a natural tendency to view the wide area around the target pixel to obtain clues of right correspondence. However, designing a matching cost function that works on a large window in the same way is difficult. The cost function is typically not intelligent enough to discard the information irrelevant to the target pixel, resulting in undesirable artifacts. In this letter, we propose a novel convolutional neural network (CNN) module to learn a stereo matching cost with a large-sized window. Unlike conventional pooling layers with strides, the proposed per-pixel pyramid-pooling layer can cover a large area without a loss of resolution and detail. Therefore, the learned matching cost function can successfully utilize the information from a large area without introducing the fattening effect. The proposed method is robust despite the presence of weak textures, depth discontinuity, illumination, and exposure difference. The proposed method achieves near-peak performance on the Middlebury benchmark.