gebhart/patrepeval huggingface dataset:
Cloning, purification, and characterization of a thermophilic ribulokinase from Anoxybacillus kestanbolensis AC26Sari (2014)

The gene encoding ribulokinase araB from Anoxybacillus kestanbolensis AC26Sari was cloned and sequenced. The recombinant protein was expressed in Escherichia coli BL21 under the control of isopropyl-\beta-D-thiogalactopyranoside-inducible T7 promoter. The enzyme, designated as AC26RK, was purified with the MagneHis Protein Purification System. The molecular mass of the native protein, as determined by SDS-PAGE, was about 61 kDa. AC26RK was active throughout a broad pH (pH 5.0-10.0) and temperature (50-75 °C) range, and it had an optimum pH of 9.0 and optimum temperature of 60 °C. The enzyme displayed about 90%-100% of its original activities after a 30-min incubation at a pH interval of 5.0-10.0. The enzyme exhibited a high level of D-ribulose activity with apparent K_m, V_{max}, and K_{cat} values of 0.94 mM, 3.197 U/mg, and 3.31 s^{-1}, respectively. AC26RK activity was strongly inhibited by Zn^{2+} but increased by Mg^{2+}. The effects of some chemicals on the ribulokinase activity revealed that Anoxybacillus kestanbolensis AC26Sari does not need metallic cations for its activity. In this paper, we describe for the first time the cloning and characterization of a thermophilic ribulokinase from thermophilic bacteria.
SEROLOGICAL STUDIES ON SUGAR (1941)

Journal Article SEROLOGICAL STUDIES ON SUGAR: II. REACTIONS OF THE ANTISERUMS OF TYPE 2 PNEUMOCOCCUS AND OF LEUCONOSTOC MESENTEBOIDES WITH CANE AND BEET SUGARS AND WITH CANE JUICE Get access JAMES M. NEILL, JAMES M. NEILL Search for other works by this author on: Oxford Academic PubMed Google Scholar JOHN Y. SUGG, JOHN Y. SUGG Search for other works by this author on: Oxford Academic PubMed Google Scholar EDWARD J. HEHRE, EDWARD J. HEHRE Search for other works by this author on: Oxford Academic PubMed Google Scholar EVELYN JAFFE EVELYN JAFFE Search for other works by this author on: Oxford Academic PubMed Google Scholar American Journal of Epidemiology, Volume 34-SectionB, Issue 2, September 1941, Pages 65–78, https://doi.org/10.1093/oxfordjournals.aje.a118736 Published: 01 September 1941 Article history Received: 22 May 1940 Published: 01 September 1941
Raman shifting of Nd:YAG laser radiation in methane: an efficient method to generate 3-μm radiation for medical uses (1989)

Generation of infrared radiation at lambda = 2.804 microm by stimulated Raman scattering of Nd:YAG laser radiation (lambda = 1.06 microm) in methane is investigated. Up to 12 mJ/pulse are obtained with 320-mJ input energy; the generated radiation shows a beam divergence of </=1 mrad. The values obtained meet the requirements established in the literature, e.g., for eye surgery.
The coupling of plasma membrane calcium entry to calcium uptake by endoplasmic reticulum and mitochondria (2013)

Cross-talk between organelles and plasma membrane Ca(2+) channels is essential for modulation of the cytosolic Ca(2+) ([Ca(2+)]C) signals, but such modulation may differ among cells. In chromaffin cells Ca(2+) entry through voltage-operated channels induces calcium release from the endoplasmic reticulum (ER) that amplifies the signal. [Ca(2+)]C microdomains as high as 20-50 μm are sensed by subplasmalemmal mitochondria, which accumulate large amounts of Ca(2+) through the mitochondrial Ca(2+) uniporter (MCU). Mitochondria confine the high-Ca(2+) microdomains (HCMDs) to beneath the plasma membrane, where exocytosis of secretory vesicles happens. Cell core [Ca(2+)]C is much smaller (1-2 μm). By acting as a Ca(2+) sink, mitochondria stabilise the HCMD in space and time. In non-excitable HEK293 cells, activation of store-operated Ca(2+) entry, triggered by ER Ca(2+) emptying, also generated subplasmalemmal HCMDs, but, in this case, most of the Ca(2+) was taken up by the ER rather than by mitochondria. The smaller size of the [Ca(2+)]C peak in this case (about 2 μm) may contribute to this outcome, as the sarco-endoplasmic reticulum Ca(2+) ATPase has much higher Ca(2+) affinity than MCU. It is also possible that the relative positioning of organelles, channels and effectors, as well as cytoskeleton and accessory proteins plays an important role.
Two new eremophilane sesquiterpene lactones from Ligularia myriocephala Ling (2005)

Two new eremophilane sesquiterpene lactones, 1 beta-angeloyloxy-6 beta, 10 beta-dihydroxy-8 beta-methoxyeremophila-7(11)en-8 alpha,12-olide and 1 beta-angeloyloxy-6 beta,10 alpha-dihydroxy-8 alpha-methoxyeremophila-7(11)en-8 beta,12-olide were isolated from the extract of the whole plant of Ligularia myriocephala Ling. Their structures and stereochemistry were elucidated by various spectroscopic methods including intensive 2D-NMR techniques (COSY, gHMQC, gHMBC and H-1-H-1 NOESY) and HR-ESIMS.
Post-acute brain injury rehabilitation for patients with stroke (2004)

While this investigation has limitations, findings suggest that PABIR is beneficial for some persons with strokes.
Environmental sensor networks in ecological research (2009)

Environmental sensor networks offer a powerful combination of distributed sensing capacity, real-time data visualization and analysis, and integration with adjacent networks and remote sensing data streams. These advances have become a reality as a combined result of the continuing miniaturization of electronics, the availability of large data storage and computational capacity, and the pervasive connectivity of the Internet. Environmental sensor networks have been established and large new networks are planned for monitoring multiple habitats at many different scales. Projects range in spatial scale from continental systems designed to measure global change and environmental stability to those involved with the monitoring of only a few meters of forest edge in fragmented landscapes. Temporal measurements have ranged from the evaluation of sunfleck dynamics at scales of seconds, to daily CO2 fluxes, to decadal shifts in temperatures. Above-ground sensor systems are partnered with subsurface soil measurement networks for physical and biological activity, together with aquatic and riparian sensor networks to measure groundwater fluxes and nutrient dynamics. More recently, complex sensors, such as networked digital cameras and microphones, as well as newly emerging sensors, are being integrated into sensor networks for hierarchical methods of sensing that promise a further understanding of our ecological systems by revealing previously unobservable phenomena.
ENDOR spectra of aminoxyls. Conformational study of alkyl and aryl spin adducts of deuterated .alpha.-phenyl-N-tert-butyl nitrone (PBN) based on proton and .beta.-carbon-13 hyperfine splittings (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTENDOR spectra of aminoxyls. Conformational study of alkyl and aryl spin adducts of deuterated .alpha.-phenyl-N-tert-butyl nitrone (PBN) based on proton and .beta.-carbon-13 hyperfine splittingsEdward G. Janzen, Uwe M. Oehler, D. Larry. Haire, and Yashige. KotakeCite this: J. Am. Chem. Soc. 1986, 108, 22, 6858–6863Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/ja00282a003https://doi.org/10.1021/ja00282a003research-articleACS PublicationsRequest reuse permissionsArticle Views138Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
IKEv2 Mobility and Multihoming Protocol (MOBIKE) (2006)

This document describes the MOBIKE protocol, a mobility and
multihoming extension to Internet Key Exchange (IKEv2). MOBIKE allows
the IP addresses associated with IKEv2 and tunnel mode IPsec Security
Associations to change. A mobile Virtual Private Network (VPN) client
could use MOBIKE to keep the connection with the VPN gateway active
while moving from one address to another. Similarly, a multihomed host
could use MOBIKE to move the traffic to a different interface if, for
instance, the one currently being used stops working. [STANDARDS-
TRACK]
Scheduling real-time transactions with disk resident data (1989)

Managing transactions with real-time requirements and disk resident data presents many new problems. In this paper we address several: How can we schedule transactions with deadlines? How do the real-time constraints affect concurrency control? How does the scheduling of IO requests affect the timeliness of transactions? How should exclusive and shared locking be handled? We describe a new group of algorithms for scheduling realtime transactions which produce serializable schedules. We present a model for scheduling transactions with deadlines on a single processor disk resident database system, and evaluate the scheduling algorithms through detailed simulation.
Cationic Polymerization (1970)

カチオン重合というと「ラジカル重合ほど親しみがない」という人が多い.しかしカチオン重合といっても何も特別の反応でない.低分子の反応で良く知られた,親電子性試薬のオレフィンへの付加反応の一つの例にすぎないという見方もできる.こう考えるとわりに気楽にカチオン重合がながめられる.また「カチオン重合は実用面から遠い反応」と感じる人も多い.しかしラジカル重合では得られないポリマーを合成できる可能性がある.例として環状エーテルの重合などがあげられる.とにかく,ある反応を通して,新しい反応,新しいポリマーの合成法を見出すためには,反応の基礎を理解しなくてはならない.そのため,ここではカチオン重合で重要な開始反応と生長反応を中心として,最近明らかになったこと,および研究されているが未解決の問題を紹介する.これらの問題を通じてカチオン重合に親しんでいただこうと思ったからである.これを機会に違った分野の方から,カチオン重合を研究している者にご批判をいただけると幸である.
Aortic Arch Reconstruction by Transluminally Placed Endovascular Branched Stent Graft (1999)

Background —Recently, thoracic aortic stent grafting has emerged as an alternative therapeutic modality for patients with thoracic aortic aneurysms and aortic dissections. However, its application has been limited to descending thoracic aortic aneurysms distal to the aortic arch. We report our initial clinical experience of endovascular branched stent graft repair for aortic arch aneurysms. Methods and Results —Endovascular grafting with Inoue branched stent grafts was attempted for 15 patients with thoracic aortic aneurysms and aortic dissections under local anesthesia (n=14) or general anesthesia (n=1). Single-branched stent grafts were used in 14 patients, and a triple-branched stent graft in one. The branched stent grafts were delivered through a 22F or a 24F sheath under fluoroscopic guidance and implanted across the aneurysmal aortic arch. In 2 patients, the single-branched stent graft did not pass through the 22F sheath used. Complete thrombosis of the aneurysm was ultimately achieved in 11 patients (73%). Of 4 persistent leaks, 1 minor leak spontaneously thrombosed and 1 major leak was successfully treated by additional straight stent graft placement. In 1 patient, the right external iliac artery ruptured during the withdrawal of the sheath and was successfully repaired by the implantation of a straight stent graft. One patient with severe stenosis of the aortic graft section was successfully managed by additional stent deployment. Peripheral microembolization to a toe occurred in 1 patient, and cerebral infarction occurred in 1 other patient. Two patients who had failed to receive endovascular stent grafts died during an average follow-up of 12.6 months, 1 of pneumonia and the other of rupture of a concomitant abdominal aortic aneurysm. Conclusions —This report demonstrates the technical feasibility of endovascular branched stent graft repair for aneurysms located at the aortic arch. Careful, longer follow-up and further extensive clinical trials are awaited toward establishing this technique as a recommendable alternative to surgical treatment of thoracic aortic aneurysms.
Electronic image archiving in the catheterization laboratory: multicenter comparison of enhanced optical storage with 1024/sup 2/ digital and 35 mm cine-film (2002)

The authors developed a bi-directional 1249/625 lines converter, able to store and to replay high resolution (1249 lines) video images, from Component Record Video (CRV) Optical Laser (Analogue) Videodisks. Image quality was evaluated four ways: (1) The authors evaluated Quantitative Coronary Angiography (QCA) on standard coronary test phantoms using enhanced CRV storage images digitally processed and also compared 78 arterial measurements ranging 0.65 to 4.85 mm both front the digital hard disc (D) and the CRV optical disc (CRV), using the same QCA analytical package. No systematic over or underestimation occurred, the mean variability was inferior to 0.1 mm and results were similar to the results obtained in the literature from digitally stored images. (2) Outside experts compared 593 static images stored on CRV discs to the same images stored in 1024/sup 2/ digital format blinded to source of image. The four experts found no visual or medical difference in 98% of evaluated images and minor differences in the remainder. (3) 90 representatives from 63 institutions compared images from both a digital hard drive and the enhanced CRV optical (analogue) storage. During the test they were blinded as to the source of the images. The representatives found no difference in image resolution, quality diagnostic accuracy, and medical relevance. (4) Two of the experts also compared the enhanced CRV optically stored image to the image obtained on simultaneously recorded 35 mm cine film, and found the enhanced CRV stored image to be superior or similar, but never inferior to the film image. It is concluded that High-Resolution CRV storage represents a cost effective solution for excellent image quality equivalent to digitally stored images, permitting permanent electronic archiving inside the cath-lab, and allowing digital image processing and digital DICOM network and CD-ROM based image communication.
Genotype of Varicella-Zoster Virus Isolates in South Korea (2011)

Information about the genotype of varicella-zoster virus (VZV) is useful to monitor outbreaks of vaccine strains. However, in South Korea, where varicella vaccine was introduced in 1988, there are limited data about the genotype of VZV. VZV was isolated from vesicular lesions of patients with herpes zoster or varicella in South Korea between January 2007 and June 2009. DNAs were purified from single-passage isolates. The genotype was determined by sequence analysis of open reading frame (ORF) 22. The PstI restriction enzyme site in ORF 38 and the BglI restriction enzyme site in ORF 54 were evaluated by restriction enzyme analysis. Forty-four patients with herpes zoster and nine patients with varicella were enrolled. The median age of patients with herpes zoster was 59.5 (range, 10 to 77) years, and the median age of patients with varicella was 8 (range, 6 to 9) years. In sequence analysis of ORF 22, all isolates were genotype J, irrespective of the age group. In restriction enzyme analysis, 51 of 54 (94.3%) isolates contained a PstI site in ORF 38, and all isolates contained a BglI site in ORF 54. Our data suggest that genotype J has been circulating since the 1940s in South Korea.
Measuring flushing symptoms with extended-release niacin using the flushing symptom questionnaire©: results from a randomised placebo-controlled clinical trial (2008)

In conclusion, the GFSS component of the FSQ was a sensitive and responsive quantitative measure of ER niacin-induced flushing that will aid in the objective comparison of novel strategies intended to improve tolerability and adherence to niacin, an agent proven to reduce cardiovascular risk.
Imaging tumors with peptide-based radioligands. (2001)

Regulatory peptides are small, readily diffusable and potent natural substances with a wide spectrum of receptor-mediated actions in humans. High affinity receptors for these peptides are (over-) expressed in many neoplasms, and these receptors may represent, therefore, new molecular targets for cancer diagnosis and therapy. This review aims to give an overview of the peptide-based radiopharmaceuticals which are presently already commercially available or which are in advanced stages of their clinical testing so that their broader availability is anticipated soon. Physiologically, these peptides bind to and act through G protein-coupled receptors in the cell membrane. Historically, somatostatin analogs are the first class of receptor binding peptides having gained clinical application. 111In-DTPA-[D-Phe1]-octreotide is the first and only radiopeptide which has obtained regulatory approval in Europe and the United States to date. Extensive clinical studies involving several thousands of patients have shown that the major clinical application of somatostatin receptor scintigraphy is the detection and the staging of gastroenteropancreatic neuroendocrine tumors (carcinoids). In these tumors, octreotide scintigraphy is superior to any other staging method. However, its sensitivity and accuracy in other, more frequent neoplasms is limited. Radiolabeled vasoactive intestinal peptide (VIP) has been shown to visualize the majority of gastrointestinal adenocarcinomas, as well as some neuroendocrine tumors, including insulinomas (the latter being often missed by somatostatin receptor scintigraphy). Due to the outstanding diagnostic accuracy of the pentagastrin test in detecting the presence, persistence, or recurrence of medullary thyroid cancer (MTC), we postulated the expression of the corresponding (ie. cholecystokinin [CCK-] -B) receptor type in human MTC. This receptor is also widely expressed on human small-cell lung cancer. Indeed, 111In-labeled DTPA derivatives of gastrin showed excellent targeting of CCK-B receptor expressing tissues in animals and patients. A variety of further peptide-based radioligands, e.g. among many others, gastrin-releasing peptide/bombesin, neurotensin, substance-P, pan-somatostatin (somatostatin derivatives which bind to all five receptor subtypes) or glucagon-like peptide-1 (glp-1) analogs (the latter for the specific detection of insulinomas), is currently under development. Summarizing, radiolabeled regulatory peptides have opened new horizons in nuclear oncology for diagnosis (and potential internal radionuclide therapy). Future work will probably reveal a multitude of novel potentially clinically useful peptide-based radioligands.
Home automation (1989)

The impact of home automation on domestic lifestyles will be as far ranging as was that of factory automation on industry and its benefits will be available to all sectors of society. Home automation will be achieved not with the household robot but with embedded computing power and memory within dozens of pieces of domestic equipment, each of which will communicate with the user and with other equipments. Within the integrated home system the communication media will include infra-red, radio, mainswires, installed twisted wires and coaxial cable, and later perhaps optical fibre. Applications will include security, lighting, heating, cooking, washing appliances, audio and video systems, energy management as well as a number ofnew applications such as health monitoring, home publishing etc. A large standards activity is in place by the major manufacturers of domestic equipment throughout Europe to ensure that their equipments are reliable and compatible.
DIRECT NUMERICAL SIMULATION: A Tool in Turbulence Research (1998)

▪ Abstract We review the direct numerical simulation (DNS) of turbulent flows. We stress that DNS is a research tool, and not a brute-force solution to the Navier-Stokes equations for engineering problems. The wide range of scales in turbulent flows requires that care be taken in their numerical solution. We discuss related numerical issues such as boundary conditions and spatial and temporal discretization. Significant insight into turbulence physics has been gained from DNS of certain idealized flows that cannot be easily attained in the laboratory. We discuss some examples. Further, we illustrate the complementary nature of experiments and computations in turbulence research. Examples are provided where DNS data has been used to evaluate measurement accuracy. Finally, we consider how DNS has impacted turbulence modeling and provided further insight into the structure of turbulent boundary layers.
Structure of Human Cytidine Deaminase Bound to a Potent Inhibitor (2005)

Human cytidine deaminase (CDA) is an enzyme prominent for its role in catalyzing metabolic processing of nucleoside-type anticancer and antiviral agents. It is thus a promising target for the development of small molecule therapeutic adjuvants. We report the first crystal structure of human CDA as a complex with a tight-binding inhibitor, diazepinone riboside 1. The structure reveals that inhibitor 1 is able to establish a canonical pi/pi-interaction with a key active site residue, Phe 137.
Effects of Seismic Acquisition Geometry on Seismic Processing - Analysis of the DMO Techniques (1995)

We have witnessed in recent years dramatic developments in the acquisition configurations deployed for Marine 3D seismic Surveying, a typical contemporary configuration may consist of dual sources and 6 x 3 km streamers covering a 250 m swath of the subsurface with each boat line. Such developments have reduced dramaticaIly the time taken for 3D acquisition and hence the cast of the technique, contributing to the widespread use of 3D surveying in exploration.
An Autonomous and Controllable Light‐Driven DNA Walking Device (2012)

Regulatable nanorobots: A DNA-based walking device was designed that is capable of autonomous locomotion, with light control of initiation, termination, and velocity (see picture). This device is reminiscent of cellular protein motors in nature, especially those of green plants.
New Tracers Identify Hydraulic Fracturing Fluids and Accidental Releases from Oil and Gas Operations (2014)

Identifying the geochemical fingerprints of fluids that return to the surface after high volume hydraulic fracturing of unconventional oil and gas reservoirs has important applications for assessing hydrocarbon resource recovery, environmental impacts, and wastewater treatment and disposal. Here, we report for the first time, novel diagnostic elemental and isotopic signatures (B/Cl, Li/Cl, δ11B, and δ7Li) useful for characterizing hydraulic fracturing flowback fluids (HFFF) and distinguishing sources of HFFF in the environment. Data from 39 HFFFs and produced water samples show that B/Cl (>0.001), Li/Cl (>0.002), δ11B (25-31‰) and δ7Li (6-10‰) compositions of HFFF from the Marcellus and Fayetteville black shale formations were distinct in most cases from produced waters sampled from conventional oil and gas wells. We posit that boron isotope geochemistry can be used to quantify small fractions (∼0.1%) of HFFF in contaminated fresh water and likely be applied universally to trace HFFF in other basins. The novel environmental application of this diagnostic isotopic tool is validated by examining the composition of effluent discharge from an oil and gas brine treatment facility in Pennsylvania and an accidental spill site in West Virginia. We hypothesize that the boron and lithium are mobilized from exchangeable sites on clay minerals in the shale formations during the hydraulic fracturing process, resulting in the relative enrichment of boron and lithium in HFFF.