gebhart/patrepeval huggingface dataset:
CD123 expression levels in 846 acute leukemia patients based on standardized immunophenotyping (2018)

Authors evaluated CD123 expression in a large cohort of acute leukemia patients, based on standardized and reproducible methodology. Our results may facilitate stratification of patients most likely to respond to CD123 targeted therapies and serve as reference for CD123 expression (in health and disease). © 2018 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.
Sensitivity analysis and parameter identifiability for colloid transport in geochemically heterogeneous porous media (2001)

Effective use of colloid transport models for heterogeneous subsurface porous media requires the development of methodologies to identify the key model parameters. The inverse problem of a two‐dimensional model for colloid transport in geochemically heterogeneous porous media is systematically investigated in this paper. Sensitivity analysis prior to the parameter identification provided valuable insights into the identifiability of the six model parameters. The hydraulic conductivity and longitudinal dispersivity were identified from tracer breakthrough data and then were used in the remaining parameter identification. The four colloid deposition and release parameters, favorable (fast) colloid deposition rate coefficient, geochemical heterogeneity, unfavorable (slow) colloid deposition rate coefficient, and colloid release rate coefficient from the unfavorable surface fraction, were found to be highly interrelated, and the inverse solution of the four‐parameter set was not unique. When either the geochemical heterogeneity or favorable colloid deposition rate coefficient is known, the other three colloid deposition and release parameters can be identified via the inverse solution if the duration of the colloid injection is sufficiently long. The colloid release rate coefficient, however, cannot be identified when using a short (pulse‐like) colloid injection. Neglecting the colloid release rate results in estimation errors of the other model parameters and thus adversely affects the subsequent prediction of colloid transport.
1,3‐Dialkoxycalix[4]arenecrowns‐6 in 1,3‐Alternate Conformation: Cesium‐Selective Ligands that Exploit Cation‐Arene Interactions (1994)

Exceptionally high selectivity for cesium ions (α(Cs/Na) > 33000) is displayed by calix[4]arenecrowns-6 in the 1,3-alternate conformation. They also remove 137Cs quantitatively (>96%) from radioactive waste that is 1 M in HNO3. The complexation properties result from the simultaneous operation of several effects: the size of the crown ether ring, the polarity of the calix conformation, and the strength of the cation/π-electron interactions. The latter interaction was evident in the X-ray crystal structure of the cesium complex (shown on the right).
Two-Photon Excitation of Flavins and Flavoproteins with Classical and Quantum Light (2018)

In this contribution, the entangled two-photon absorption (ETPA) process on naturally occurring flavoproteins was studied. Low temperature responsive protein (LOT6P) and b-type dihydroorotate dehydrogenase (DHOD B), which possess flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) chromophores embedded in the protein environment, were investigated. The ETPA cross-section was measured, and we found that it increases when going from an aqueous solution of the free flavin chromophore to the chromophore embedded in the protein. This enhancement is particularly evident when entangled photons are used as excitation light compared to classical light. Our results prove the potential of ETPA as a sensing technique for fluorescent proteins even for those whose classical TPA cross-section is small compared to well-known fluorescent proteins.
Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: A marriage made in heaven (1999)

Carboxyfluorescein diacetate succinimidyl ester (CFSE) labelling of naïve lymphocyte populations provides unique insights into the immune response. The clonal nature of immune responses, necessitating clonal expansion to achieve a sufficiently large number of Ag-reactive effector cells, combined with the dependence of lymphocyte differentiation on cell division, underlie the usefulness of CFSE in understanding the factors that regulate responses both in vitro and in vivo. We have combined CFSE labelling with Ag receptor transgenic models, using seven channel flow cytometry to track the correlation between cell division and a number of other parameters, such as surface expression of activation markers, cytokine receptors and homing receptors, cytokine production, cytotoxic activity and indicators of apoptosis. Our data have allowed us to classify and understand immune responses in novel ways, suggesting many further avenues of enquiry and indicating previously unrecognized relationships between cell division and eventual cell fate.
Cell Surface Major Histocompatibility Complex Class II Proteins Are Regulated by the Products of the γ<sub>1</sub>34.5 and U<sub>L</sub>41 Genes of Herpes Simplex Virus 1 (2002)

Modulation of host immune responses has emerged as a common strategy employed by herpesviruses both to establish life-long infections and to affect recovery from infection. Herpes simplex virus 1 (HSV-1) blocks the major histocompatibility complex (MHC) class I antigen presentation pathway by inhibiting peptide transport into the endoplasmic reticulum. The interaction of viral gene products with the MHC class II pathway, however, has not been thoroughly investigated, although CD4(+) T cells play an important role in human recovery from infection. We have investigated the stability, distribution, and state of MHC class II proteins in glioblastoma cells infected with wild-type HSV-1 or mutants lacking specific genes. We report the following findings. (i) Wild-type virus infection caused a decrease in the accumulation of class II protein on the surface of cells and a decrease in the endocytosis of lucifer yellow or dextran conjugated to fluorescein isothiocyanate but no decrease in the total amount of MHC class II proteins relative to the levels seen in mock-infected cells. (ii) Although the total amount of MHC class II protein remained unchanged, the amounts of cell surface MHC class II proteins were higher in cells infected with the U(L)41-negative mutant, which lacks the virion host shutoff protein, and especially high in cells infected with the gamma(1)34.5-negative mutant. We conclude that infected cells attempt to respond to infection by increased acquisition of antigens and transport of MHC class II proteins to the cell surface and that these responses are blocked in part by the virion host shutoff protein encoded by the U(L)41 gene and in large measure by the direct or indirect action of the infected cell protein 34.5, the product of the gamma(1)34.5 gene.
Molecular Epidemiology of an SHV-5 Extended-Spectrum  -Lactamase in Enterobacteriaceae Isolated from Infants in a Neonatal Intensive Care Unit (1995)

Klebsiella oxytoca that produced extended-spectrum beta-lactamase (ESBL) and were resistant to ceftazidime were isolated from infants in a neonatal intensive care unit (NICU). During a 30-week period, 3 infants developed infections and an additional 60 infants were colonized with these bacteria. The molecular typing data suggested transmission of a single strain of ceftazidime-resistant K. oxytoca among 48 of the 63 infants. The ESBL of 46 of the 48 similar isolates, 14 of the remaining 15 isolates, and 6 other Enterobacteriaceae appeared to be associated with a conjugative plasmid of approximately 85 kb. The ESBL gene was cloned, and DNA sequencing confirmed that the ESBL was an SHV-5. Hybridization data suggested that the SHV-5 gene was transmitted to other Enterobacteriaceae in vivo. The spread of the ESBL was reduced through adherence to infection control practices.
Biochemical nature of an Fc mu receptor on human B-lineage cells. (1990)

An IgM-binding protein of approximately 60 kD has been identified on activated B cells, but not on resting and activated T cells, monocytes, or granulocytes. Here, we characterize this IgM-binding protein as a receptor for the Fc portion (CH3 and/or CH4 domains) of IgM molecules (Fc microR). The Fc microR can be expressed as a cell surface activation antigen throughout the pre-B and B cell stages in differentiation. Receptor expression is not directly linked with IgM production, as both mu- pre-B cells and isotype-switched B cells may express the Fc microR. The receptor molecules produced by both pre-B and B cells are identical in size and are characterized as an acidic sialoglycoprotein with O-linked, but no N-linked, oligosaccharide. The Fc microR is anchored to the surface of B-lineage cells via a glycosyl phosphatidylinositol linkage. The Fc microR is thus the third member of a family of Fc receptors expressed on B-lineage cells, and its preferential expression on activated B cells suggests a potential role in the response to antigens.
Stoffwechselprodukte von Actinomyceten. 3. Mitteilung. Nonactin (1955)

Abstract Aus Kulturfiltrat und Mycel eines Streptomyces viridochromogenes oder Streptomyces olivochromogenes nahestehenden Streptomyceten wurde neben Actidion eine neutrale, farblose, kristalline Verbindung C 30 H 48 O 9 , das Nonactin, isoliert, die chemisch wenig reaktiv ist und gegenüber den untersuchten Mikroorganismen unwirksam oder nur schwach wirksam war.
Comparison of classifier methods: a case study in handwritten digit recognition (2002)

This paper compares the performance of several classifier algorithms on a standard database of handwritten digits. We consider not only raw accuracy, but also training time, recognition time, and memory requirements. When available, we report measurements of the fraction of patterns that must be rejected so that the remaining patterns have misclassification rates less than a given threshold.
Post-Translational Modifications of Natural Antimicrobial Peptides and Strategies for Peptide Engineering (2012)

Natural antimicrobial peptides (AMPs) are gene-coded defense molecules discovered in all the three life domains: Eubacteria, Archaea, and Eukarya. The latter covers protists, fungi, plants, and animals. It is now recognized that amino acid composition, peptide sequence, and post-translational modifications determine to a large extent the structure and function of AMPs. This article systematically describes post-translational modifications of natural AMPs annotated in the antimicrobial peptide database (http://aps.unmc.edu/AP). Currently, 1147 out of 1755 AMPs in the database are modified and classified into more than 17 types. Through chemical modifications, the peptides fold into a variety of structural scaffolds that target bacterial surfaces or molecules within cells. Chemical modifications also confer desired functions to a particular peptide. Meanwhile, these modifications modulate other peptide properties such as stability. Elucidation of the relationship between AMP property and chemical modification inspires peptide engineering. Depending on the objective of our design, peptides may be modified in various ways so that the desired features can be enhanced whereas unwanted properties can be minimized. Therefore, peptide design plays an essential role in developing natural AMPs into a new generation of therapeutic molecules.
Inflammatory Bowel Disease: MR- and SPECT/CT-based Macrophage Imaging for Monitoring and Evaluating Disease Activity in Experimental Mouse Model—Pilot Study (2014)

SPIO nanoparticles and (111)In-labeled macrophages could be observed in vivo at MR imaging and SPECT/CT in mice with IBD. Percentage NE at MR imaging correlates with disease activity.
Comparative dose-effect relations at several dosing intervals for beta-lactam, aminoglycoside and quinolone antibiotics against gram-negative bacilli in murine thigh-infection and pneumonitis models. (1990)

Relatively few animal studies have investigated the influence of dosing regimens on the efficacy of antibiotics possessing different pharmacodynamic characteristics. We evaluated the impact of dosing interval on the relative efficacy and potency of beta-lactams, aminoglycosides, and ciprofloxacin against Pseudomonas aeruginosa. Escherichia coli, and Klebsiella pneumoniae in murine pneumonitis and thigh-infection models. We used a sigmoid dose-response model to determine the maximal bactericidal effect at 24 hours (Emax) and the total dose required to achieve 50% of Emax (P50) at several dosing intervals. P50S (a measure of drug potency, in mg/kg/day) for beta-lactams increased 14- to 73-fold with longer intervals in both models. Dosing interval had little impact on P50S for aminoglycosides or ciprofloxacin. Emax varied among drugs but displayed no dependence on dosing interval. This method of analysis allows comparison of efficacy and dependence of potency on dosing regimen among different classes of antibiotics.
Building pattern recognition in topographic data: examples on collinear and curvilinear alignments (2011)

Building patterns are important features that should be preserved in the map generalization process. However, the patterns are not explicitly accessible to automated systems. This paper proposes a framework and several algorithms that automatically recognize building patterns from topographic data, with a focus on collinear and curvilinear alignments. For both patterns two algorithms are developed, which are able to recognize alignment-of-center and alignment-of-side patterns. The presented approach integrates aspects of computational geometry, graph-theoretic concepts and theories of visual perception. Although the individual algorithms for collinear and curvilinear patterns show great potential for each type of the patterns, the recognized patterns are neither complete nor of enough good quality. We therefore advocate the use of a multi-algorithm paradigm, where a mechanism is proposed to combine results from different algorithms to improve the recognition quality. The potential of our method is demonstrated by an application of the framework to several real topographic datasets. The quality of the recognition results are validated in an expert survey.
Anti-human CD40 monoclonal antibody therapy is potent without FcR crosslinking (2014)

Antibody agonists targeting tumor necrosis factor (TNF) superfamily receptors, including CD40, are being tested therapeutically as anticancer agents. Studies in mice have shown that anti-CD40 monoclonal antibody (mAb) requires Fc-receptor (FcR) engagement to activate antitumor immunity. In contrast, we have reported that clinically active anti-human CD40 mAb CP-870,893 does not require FcR crosslinking, a finding with translational implications.
α- and β-Adducin polymorphisms affect podocyte proteins and proteinuria in rodents and decline of renal function in human IgA nephropathy (2009)

Adducins are cytoskeletal actin-binding proteins (alpha, beta, gamma) that function as heterodimers and heterotetramers and are encoded by distinct genes. Experimental and clinical evidence implicates alpha- and beta-adducin variants in hypertension and renal dysfunction. Here, we have addressed the role of alpha- and beta-adducin on glomerular function and disease using beta-adducin null mice, congenic substrains for alpha- and beta-adducin from the Milan hypertensive (MHS) and Milan normotensive (MNS) rats and patients with IgA nephropathy. Targeted deletion of beta-adducin in mice reduced urinary protein excretion, preceded by an increase of podocyte protein expression (phospho-nephrin, synaptopodin, alpha-actinin, ZO-1, Fyn). The introgression of polymorphic MHS beta-adducin locus into MNS (Add2, 529R) rats was associated with an early reduction of podocyte protein expression (nephrin, synaptopodin, alpha-actinin, ZO-1, podocin, Fyn), followed by severe glomerular and interstitial lesions and increased urinary protein excretion. These alterations were markedly attenuated when the polymorphic MHS alpha-adducin locus was also present (Add1, 316Y). In patients with IgA nephropathy, the rate of decline of renal function over time was associated to polymorphic beta-adducin (ADD2, 1797T, rs4984) with a significant interaction with alpha-adducin (ADD1, 460W, rs4961). These findings suggest that adducin genetic variants participate in the development of glomerular lesions by modulating the expression of specific podocyte proteins.
New Testament Use of the Old Testament (1994)

U ovome članku moje razumijevanje ovog predmeta preraslo je u čvrsto uvjerenje da novozavjetni citati starozavjetnih redaka ne uključuju „kristološku reinterpretaciju“, helenističku alegoriju, rabinski midraš, kumransku vrstu pešera, ili druge metode tumačenja koje iskrivljuju izvorno značenje starozavjetnih citata. Umjesto toga, novozavjetni pisci ostaju dosljedni i vjerni izvornim redcima u neposrednom i širem starozavjetnom kontekstu.
High-throughput plasmid DNA purification for 3 cents per sample (1999)

To accommodate the increasingly rapid rates of DNA sequencing we have developed and implemented an inexpensive, expeditious method for the purification of double-stranded plasmid DNA clones. The robust nature, high throughput, low degree of technical difficulty and extremely low cost have made it the plasmid DNA preparation method of choice in both our expressed sequence tag (EST) and genome sequencing projects. Here we report the details of the method and describe its application in the generation of more than 700 000 ESTs at a rate exceeding 16 000 per week.
Carbohydrate Metabolism and Insulin. (1926)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
Calcium, Phosphorus, and Bone in Renal Disease and Transplantation (1969)

Calcium-phosphate-bone interrelationships become disrupted progressively in advancing renal disease. Parathyroid hormone (PTH) release, normally attuned to calcium homeostasis requirements, becomes dominated by alterations in phosphate balance. With each wave of nephron destruction, there is believed to occur transient hyperphosphatemia, transient hypocalcemia, and a step-wise rise in PTH levels. When the glomerular filtration rate falls below 25 to 30 ml per minute, hyperphosphatemia persists, hypocalcemia tends to persist, and the stimulus to PTH release is exaggerated. Vitamin D resistance contributes both to hypocalcemia (thus to accelerated PTH secretion) and to osteomalacia. In patients receiving long-term hemodialysis, if hyperphosphatemia and hypocalcemia persist, PTH levels may remain high and osteitis fibrosa may progress. Successful renal transplantation typically leads to regression of hyperparathyroidism and restoration of vitamin D sensitivity; however, hyperparathyroidism may regress slowly, and severe hypercalcemia must be guarded against. True autonomy of PTH release apparently occurs rarely in chronic renal disease.
Increasing believability in animated pedagogical agents (1997)

Article Free Access Share on Increasing believability in animated pedagogical agents Authors: James C. Lester Multimedia Laboratory, Department of Computer Science, North Carolina State University, Raleigh, NC Multimedia Laboratory, Department of Computer Science, North Carolina State University, Raleigh, NCView Profile , Brian A. Stone Multimedia Laboratory, Department of Computer Science, North Carolina State University, Raleigh, NC Multimedia Laboratory, Department of Computer Science, North Carolina State University, Raleigh, NCView Profile Authors Info & Claims AGENTS '97: Proceedings of the first international conference on Autonomous agentsFebruary 1997 Pages 16–21https://doi.org/10.1145/267658.269943Online:08 February 1997Publication History 82citation762DownloadsMetricsTotal Citations82Total Downloads762Last 12 Months50Last 6 weeks7 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Risk Factors for Physical Restraint Use in Nursing Homes: Pre- and Post-Implementation of the Nursing Home Reform Act (1997)

The purposes of this article are (1) to identify resident and facility risk factors for the use of physical restraints since the implementation of the Nursing Home Reform Act (NHRA), and (2) to compare these results with resident and facility risk factors for the use of physical restraints prior to the implementation of the NHRA. Data were collected in 1990 and 1993, and represent residents drawn from 268 facilities in 10 states. In the 1993 cohort activities of daily living (ADL) scores, cognitive performance scale (CPS) scores, age, taking antipsychotic medications, a history of falls, and mobility problems were resident-level variables significantly associated with the use of restraints. Full-time-equivalent (FTE) nurse aides per resident, FTE RNs per resident, average occupancy, Herfindahl index, Alzheimer's special care unit, and Medicaid reimbursement policy were facility-level variables significantly associated with the use of restraints. When the 1990 cohort was compared to the 1993 cohort (pre- and post-NHRA), however, only three risk factors for the use of restraints were significant. We propose that, to date, the NHRA may have been successful in reducing the use of physical restraints, but it appears to have had less impact on the types of residents who are restrained.
Comparative neurophysiology of spatial luminance contrast sensitivity. (2011)

The luminance contrast sensitivity function has been investigated using behavioral and electrophysiological methods in many vertebrate species. Some features are conserved across species as a shape of the function, but other features, such as the contrast sensitivity peak value, spatial frequency contrast sensitivity peak, and visual acuity have changed. Here, we review contrast sensitivity across different classes of vertebrates, with an emphasis on the frequency contrast sensitivity peak and visual acuity. We also correlate the data obtained from the literature to test the power of the association between visual acuity and the spatial frequency of the contrast sensitivity function peak.