gebhart/patrepeval huggingface dataset:
HIV-1 Residual Viremia and Proviral DNA in Patients with Suppressed Plasma Viral Load ( < 400 HIV-RNA cp/ml) During Different Antiretroviral Regimens (2008)

Low levels of plasma viremia (below 50 copies/ml of HIV-1 RNA) can be detected in the majority of HIV+ subjects successfully treated with HAART. Aim of our study was to evaluate the impact of different antiretroviral regimens on this residual viremia and on proviral HIV-1 DNA in HAART-treated subjects with plasma HIV RNA <400 cp/ml and no history of virological failure. To this purpose, a cross-sectional analysis of 319 HIV-positive patients on HAART with plasma HIV RNA <400 cp/ml was performed. Subjects had been on HAART for a median of 3.6 years: the current regimen included two nucleoside reverse transcriptase inhibitors (NRTIs) plus a protease inhibitor (PI) in 104 (32.6%) cases, of which 73 treated with a boosted PI; two NRTIs plus a non-NRTI (NNRTI) were prescribed in 166 (52.2%) cases, and NRTIs-only in 49 cases (15.4%). Patients treated with PI had the lowest nadir CD4 cell count (237+191 cells/microl) compared to patients treated with NNRTI (384+192 cells/microl) or NRTIs-only (387+222 cells/microl). Cell-associated HIV-1 DNA was measured in 231 subjects. Residual viremia was measured in 238 subjects with plasma HIV-1 RNA levels < 50 copies/ml. Multivariate analysis showed that the use of NNRTI was independently associated to low levels of residual viremia and high levels of HIV-1DNA, whereas the use of PI was independently associated to low levels of HIV-1 DNA. The better virological performance of NNRTI in terms of low residual viremia is consistent with specific literature data, whereas the greater impact of PI on the viral reservoirs is noteworthy and needs further investigations.
Local Feature Hashing for face recognition (2009)

In this paper, we present Local Feature Hashing (LFH), a novel approach for face recognition. Focusing on the scalability of face recognition systems, we build our LFH algorithm on the p-stable distribution Locality-Sensitive Hashing (pLSH) scheme that projects a set of local features representing a query image to an ID histogram where the maximum bin is regarded as the recognized ID. Our extensive experiments on two publicly available databases demonstrate the advantages of our LFH method, including: i) significant computational improvement over naive search; ii) hashing in high-dimensional Euclidean space without embedding; and iii) robustness to pose, facial expression, illumination and partial occlusion.
Evaluation of CPS ID2 medium for detection of urinary tract bacterial isolates in specimens from a rehabilitation center (1995)

CPS ID2 medium (bioMérieux) enables the presumptive identification of Escherichia coli and enterococci as well as the detection of indologenous or nonindologenous Proteeae and bacteria belonging to the Klebsiella, Enterobacter, and Serratia group with a specificity ranging from 98 to 100%. When the cultures were polymicrobial, the sensitivity varied from 70 to 97%, and the sensitivity varied from 97 to 100% when they were monomicrobial.
Broad-band determination of the FET small-signal equivalent circuit (1990)

A method to determine the broadband small-signal equivalent circuit of field-effect transistors (FETs) is proposed. This method is based on an analytic solution of the equations for the Y parameters of the intrinsic device and allows direct determination of the circuit elements at any specific frequency or averaged over a frequency range. The validity of the equivalent circuit can be verified by showing the frequency independence of each element. The method can be used for the whole range of measurement frequencies and can be applied to devices exhibiting severe low-frequency effects.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Structure-Function Relationships in Staphylokinase as Revealed by "Clustered Charge to Alanine" Mutagenesis (1995)

Eighteen mutants of recombinant staphylokinase (SakSTAR) in which clusters of two or three charged residues were converted to alanine ("clustered charge-to-alanine scan") were characterized. Fifteen of these mutants had specific plasminogen-activating activities of > 20% of that of wild-type SakSTAR, whereas three mutants, SakSTAR K11A D13A D14A (SakSTAR13), SakSTAR E46A K50A (SakSTAR48), and SakSTAR E65A D69A (SakSTAR67) had specific activities of 3%. SakSTAR13 had an intact affinity for plasminogen and a normal rate of active site exposure in equimolar mixtures with plasminogen. The plasmin-SakSTAR13 complex had a 14-fold reduced catalytic efficiency for plasminogen activation but was 5-fold more efficient for conversion of plasminogen-SakSTAR13 to plasmin-SakSTAR13. SakSTAR48 and SakSTAR67 had a 10-20-fold reduced affinity for plasminogen and a markedly reduced active site exposure; their complexes with plasmin had a more than 20-fold reduced catalytic efficiency toward plasminogen. Thus, plasminogen activation by catalytic amounts of SakSTAR is dependent on complex formation between plasmin(ogen) and SakSTAR, which is deficient with SakSTAR48 and SakSTAR67, but also on the induction of a functional active site configuration in the plasmin-SakSTAR complex, which is deficient with all three mutants. These findings support a mechanism for the activation of plasminogen by SakSTAR involving formation of an equimolar complex of SakSTAR with traces of plasmin, which converts plasminogen to plasmin and, more rapidly, inactive plasminogen-SakSTAR to plasmin-SakSTAR.
[Clinical usefulness of urinary anti HIV antibody test--a large scale study from 11 institutes in Japan]. (1995)

An enzyme immuno assay kit has been developed to detect anti-HIV antibody in urine. In order to examine the clinical utility of the kit, 1333 urine samples were assayed. These samples consisted of 233 urine samples from HIV infected patients, 472 samples from HIV uninfected patients including 203 samples from patients with urogenital diseases, and 628 samples from normal subjects. Anti-HIV antibodies were detected in all the urine samples from HIV infected patients, and the diagnostic sensitivity for HIV infection was 100% with no false negative cases. A variety of anti-HIV antibody titers were found in the urine samples from HIV infected patients. However, no significant differences were found in the distribution patterns of urinary anti-HIV antibody titers among AC, ARC and AIDS patients. False positives were determined in only five samples in 628 healthy subjects (0.8%), one in 19 patients with hepatitis (5.3%), one in 45 patients with hemophilia (2.2%) and two in 105 pregnant women (1.9%). The antibody titers of all the false positive samples in these groups were less than the cut-off index multiplied by two. However, relatively high positive rates were demonstrated in the samples from urogenital diseases (11.8%), diabetes mellitus (20.0%) and auto-immune diseases (7.3%). False positive results were found to be directly correlated to the protein concentration of urinary protein, especially the immunoglobulin concentration in urine. The assay system was also evaluated by various reproducibility tests performed by different operators at different laboratories. The test results were satisfactory.(ABSTRACT TRUNCATED AT 250 WORDS)
Temperature dependence of optical constants for InSb films including molten phases (2008)

We have measured the temperature dependence of optical constants of InSb films including molten phases. It has been found that the optical constants differ between the as-sputtered amorphous and the molten states. Also, the optical constants exhibit the large change associated with the crystalline-to-molten phase transition. In the crystalline states, the extinction coefficient increases as the temperature increases. This temperature dependence can be explained by the decrease of the band gap energy.
Automated module fabrication (1994)

The Automated Module Fabrication (AMF) line was developed to improve quality and reduce the cost of custom microwave modules, analog and digital hybrids and MCM's. The approach stresses flexibility and the ability to run small or large lot sizes with the efficiency normally only associated with mass production. Processes included are computer automated die inspection and sort, epoxy and solder die attach, a robotic wire bonding workcell, automated carrier probing and related process monitors such as X-ray void detection and wire pull. A custom computer integrated manufacturing system was developed to interface with the equipment and provide paperless documentation. This report details the work performed, problem areas encountered, and some unique solutions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Evaluation of an immunochromatographic assay for rapid detection of <i>Salmonella enterica</i> serovars Typhimurium and Enteritidis (2011)

An immunochromatographic assay was developed to detect Salmonella enterica serovars Typhimurium and Enteritidis in a single strip. The assay was constructed in the form of a sandwich, using 2 specific anti-S. Typhimurium and anti-S. Enteritidis antibodies immobilized on a nitrocellulose membrane at separated test lines, while the other specific antibody to Salmonella spp. was conjugated with gold nanoparticles. The test strips can immediately detect S. Typhimurium and S. Enteritidis specifically in a culture medium at levels as low as 10(4) and 10(6) cfu/ml, respectively. The contamination of S. Typhimurium and S. Enteritidis at 1 cfu/ml or greater can be detected by the test strips after 6-24 hr incubation. The specificities of the test strips to detect S. Typhimurium and S. Enteritidis, in spiked samples, were obtained at 100%; the sensitivities were at 98.89% (89/90) and 87.50% (70/80), respectively, compared with the conventional method. The newly developed multiplex immunochromatographic assay is the first report on the efficient detection of both S. Typhimurium and S. Enteritidis simultaneously in single strip. This test strip also provides advantages of simplicity and very rapid detection of these specific bacterial contaminants in chicken and can be useful for mass detection on chicken farms and in other veterinary products.
Inactivation of the Mitogen-Activated Protein Kinase Pathway as a Potential Target-Based Therapy in Ovarian Serous Tumors with KRAS or BRAF Mutations (2005)

Activation of mitogen-activated protein kinase (MAPK) occurs in response to various growth stimulating signals and as a result of activating mutations of the upstream regulators, KRAS and BRAF, which can be found in many types of human cancer. To investigate the roles of MAPK activation in tumors harboring KRAS or BRAF mutations, we inactivated MAPK in ovarian tumor cells using CI-1040, a compound that selectively inhibits MAPK kinase, an upstream regulator of MAPK and thus prevents MAPK activation. Profound growth inhibition and apoptosis were observed in CI-1040-treated tumor cells with mutations in either KRAS or BRAF in comparison with the ovarian cancer cells containing wild-type sequences. Long serial analysis of gene expression identified several differentially expressed genes in CI-1040-treated MPSC1 cells harboring an activating mutation in BRAF (V599L). The most striking changes were down-regulation of cyclin D1, COBRA1, and transglutaminase-2 and up-regulation of tumor necrosis factor-related apoptosis-induced ligand, thrombospondin-1, optineurin, and palladin. These patterns of gene expression were validated in other CI-1040-treated tumor cells based on quantitative PCR. Constitutive expression of cyclin D1 partially reversed the growth inhibitory effect of CI-1040 in MPSC1 cells. Our findings indicate that an activated MAPK pathway is critical in tumor growth and survival of ovarian tumors with KRAS or BRAF mutations and suggest that the CI-1040 induced phenotypes depend on the mutational status of KRAS and BRAF in ovarian tumors.
Magnetically controlled rotation and torque of uniaxial microactuators for lab-on-a-chip applications (2009)

We demonstrate the controlled rotation and torque generated by uniaxial magnetic microactuators formed by two bound superparamagnetic particles in a fluid. The torque and rotation are precisely controlled by rotating magnetic fields, generated by an external electromagnet or by on-chip current wires. We present the magnetic energy equations and the equations of motion for two-particle microactuators, with contributions from the permanent and induced magnetic moments of the particles. A comparison of theory and experiments allows an estimation of the different moments with accuracy better than 10% across a wide frequency range. At low frequencies and low magnitudes of the applied magnetic field, both the permanent and induced moments of the particles have contributions to the torque. At either high fields or high frequencies, the torque is dominated by the induced moment. The predictability of the torque is highest in the regime of low frequencies and high field, where the torque has a large magnitude and is determined by the magnetic shape anisotropy of the microactuator. A comparison of rotation in bulk fluid and on a chip surface shows an increase of friction by a factor 9 originating from the surface proximity. The detailed understanding of the torque and rotation of two-particle uniaxial magnetic microactuators opens a range of possibilities in lab-on-a-chip applications, such as the actuation of single molecules, fluid mixing in microfluidic chambers, and novel cluster-based assays.
Extraction Of 3D Measurements From Stereo X-ray Images (2005)

Stereoi or two-eyed, image processing technique has been widely used in many fields such as biostereometrics, street intersection designs; forest resource studies and so on. In 1980 Zhang et al developed a stereo X-ray imaging system with a simple structure and clear steroescopic effect which has been stereo X-ray applied to the diagnosis in medicine. This paper based on the stereo X-ray imaging system) has made a further research into the space features of stereo X-ray images. A method for extracting three- dimensional measurements from stereo X-ray images has been given. The method consists of imaging modelycorrection of the distortion resulted from the system non-linearity and point match of stereo X-ray images. The obtained results have shown the relative error of measuring depth is less than 0.7% at distance of 550.0 mm.
Percutaneous Absorption (2005)

Mechanisms of absorption: mathematical models in percutaneous absorption cutaneous metabolism during in vitro percutaneous absorption cutaneous metabolism of Xenobiotics occlusion does not uniformly enhance penetration in vivo regional variationin percutaneous absorption. Methodology: in vivo methods for percutaneous absorption measurements determination of percutaneous absorption by in vitro techniques will cutaneous levels of absorbed material be systemically absorbed dermaldecontamination and percutaneous absorption partioning of chemicals from water into powdered human stratum corneum (Callus) - a model study in vitro percutaneous absorption of model compounds glyphosate and malathion from fabric into and through humanskin vaginal absorption of prostaglandin E, in rhesus monkey human cadaver skin viability for in vitro percutaneous absorption - storage and detrimental effects of heat separation and freezing. Drug and cosmetic absorption: percutaneous penetration as amethod of delivery to skin and underlying tissues phonophoresis facilitated transdermal delivery by iontophoresis percutaneous penetration as it relates to the safety evaluation of cosmetic ingredients percutaneous absorption of fragrances hair dyeabsorption percutaneous absorption of alpha-hydroxy acids in human skin optimizing patch test delivery - a model permeation of N-nitrosamines through human skin nail penetration - focus on topical delivery of antifungal drugs for onchomycosistreatment topical dermatological vehicles - a holistic approach.
Alkylation of enamines derived from sterically hindered amines (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlkylation of enamines derived from sterically hindered aminesThomas J. Curphey, Jennie C. Y. Hung, and Christine C. C. ChuCite this: J. Org. Chem. 1975, 40, 5, 607–614Publication Date (Print):March 1, 1975Publication History Published online1 May 2002Published inissue 1 March 1975https://pubs.acs.org/doi/10.1021/jo00893a014https://doi.org/10.1021/jo00893a014research-articleACS PublicationsRequest reuse permissionsArticle Views1165Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The quest for wholeness in riders in the chariot (1975)

Like all of Patrick White's major novels, Riders in the Chariot contrasts the lives of the spiritually gifted and the spiritually impoverished. What makes reading it unusually demanding is its wealth of allusion. Despite everything critics have written about the connectedness of the elements within White's broadly religious frame of reference - so that echoes from Genesis Exodus, Esther, Daniel, Isaiah, Ezekiel, the Gospels, the Mishnah (Jewish law), the Zohar (a central Kabbalistic text), Blake Kierkegaard, and Jung seem as natural as they are apt - there are readers who consider his religious symbolism artificial, and who believe that its presence in the novel signals White's scepticism about the value of religious - and especially mystical - experience.
Protein Hydration, Thermodynamic Binding, and Preferential Hydration (2002)

ADVERTISEMENT RETURN TO ISSUECurrent Topics/Persp...Current Topics/PerspectivesNEXTProtein Hydration, Thermodynamic Binding, and Preferential HydrationSerge N. TimasheffView Author Information Department of Biochemistry, MS 009, Brandeis University, 415 South Street, Waltham, Massachusetts 02453-2728 Cite this: Biochemistry 2002, 41, 46, 13473–13482Publication Date (Web):October 26, 2002Publication History Received29 April 2002Published online26 October 2002Published inissue 1 November 2002https://pubs.acs.org/doi/10.1021/bi020316ehttps://doi.org/10.1021/bi020316ereview-articleACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views3876Altmetric-Citations419LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Free energy,Hydration,Molecules,Solvents,Thermodynamics Get e-Alerts
Plasmid DNA in the erythromycin producing microorganism, Streptomyces erythreus NRRL 2338. (1982)

Streptomyces erythreus NRRL 2338, the erythromycin producing microorganism, contains extrachromosomal (plasmid) DNA. Four different plasmids, pSE1, pSE2, pSE4 and pSE6 present in the wild-type strain have characteristic mobilities on agarose gel electrophoresis, molecular weight and restriction endonuclease digestion patterns. Treatment of the wild-type strain with ethidium bromide or acridine orange gave two variants, one that could not convert erythronolide B to 3 (alpha)-mycarosylerythronolide B and another than produced 2 approximately 3 times more erythromycin A than the parental strain. Although the plasmid DNA profile of these two variants is different from the wild-type strain, it is not possible to conclude that any of the structural genes for erythromycin biosynthesis are located on the plasmids of S. erythreus NRRL 2338.
Guide to Bluetooth Security: Recommendations of the National Institute of Standards and Technology (Special Publication 800-121 Revision 1) (2012)

The National Institute of Standards and Technology Special Publication 800-121 Revision 1, Guide to Bluetooth Security is the first revision to NIST SP 800-121, Guide to Bluetooth Security. Bluetooth is an open standard for short-range radio frequency communication. Bluetooth technology is used primarily to establish wireless personal area networks. It has been integrated into many types of business and consumer devices, including cellular phones, personal digital assistants, laptops, automobiles, printers, and headsets. This publication provides information on the security capabilities of Bluetooth and gives recommendations to organizations employing Bluetooth technologies on securing them effectively. Updates in this revision include the latest vulnerability mitigation information for Secure Simple Pairing, introduced in Bluetooth v2.1 + Enhanced Data Rate (EDR), as well as an introduction to and discussion of Bluetooth v3.0 + High Speed and Bluetooth v4.0 security mechanisms and recommendations.~
Nucleotide Sequence Analysis of the T24 Human Bladder Carcinoma Oncogene (1983)

The nucleotide sequence of the T24 human bladder carcinoma oncogene was determined, and the coding and noncoding sequences of the genome were identified. The amino acid sequence of p21, the translational product of the T24 oncogene, was predicted from the nucleotide sequence of the oncogene. Comparison of this sequence with that of the normal cellular homolog showed that a single point mutation in the coding sequences of the T24 oncogene resulted in the acquisition of transforming properties. Other differences between the T24 oncogene and its normal cellular homolog were found in the 5' noncoding and 3' noncoding sequences, but these differences appear to be due to polymorphism and do not play a significant role in the transformation process.
Induction of Delayed Hypersensitivity to Human Tumor Cells With a Human Monoclonal Anti-idiotypic Antibody (1991)

There is considerable interest in the development of anti-idiotypic antibodies as vaccines in a number of diseases, including cancer. We have developed a human anti-idiotypic monoclonal antibody (105AD7) which binds at or very near to the binding site of mouse antitumor monoclonal antibody 791T/36. The 791T/36 antibody binds to a tumor-associated antigen (gp72) expressed on a number of human tumors, including colorectal and ovarian carcinomas and osteogenic sarcoma. This study shows that, in rats and mice, 105AD7 induces delayed-type hypersensitivity to human tumor cells bearing the gp72 antigen. Local transfer of delayed hypersensitivity was also demonstrated using lymphocytes from mice primed with 105AD7. These findings show that the human monoclonal anti-idiotypic antibody 105AD7 is likely to induce cellular immune responses to tumors in cancer patients.