gebhart/patrepeval huggingface dataset:
Selective Capture and Identification of Pathogenic Bacteria Using an Immobilized Siderophore (2010)

Rapid identification of infectious pathogens constitutes an important step toward limiting the spread of contagious diseases. Whereas antibody-based detection strategies are often selected because of their speed, mutation of the pathogen can render such tests obsolete. In an effort to develop a rapid yet mutation-proof method for pathogen identification, we have explored the use of "immutable ligands" to capture the desired microbe on a detection device. In this "proof-of-principle" study, we immobilize pyoverdine, a siderophore that Pseudomonas aeruginosa must bind to obtain iron, onto gold-plated glass chips and then examine the siderophore's ability to capture P. aeruginosa for its subsequent identification. We demonstrate that exposure of pyoverdine-coated chips to increasing dilutions of P. aeruginosa allows detection of the bacterium down to concentrations as low as 10(2)/mL. We further demonstrate that printing of the siderophore in a periodic pattern on the detection chip enables a sensitive method of detecting the bound pathogen by a Fourier transform analysis of light scattered by the patterned chip. Because unrelated bacteria are not captured on the pyoverdine chip, we conclude that pyoverdine can be exploited for the specific binding and identification of P. aeruginosa. It follows that the utilization of other microbe-specific "immutable ligands" may allow the specific identification of their cognate pathogens.
Inhomogene Netzwerke durch Unverträglichkeit (1978)

Abstract Copolymerisate aus Methylacrylat (MA) bzw. n‐Hexylmethacrylat (HMA) und 4,4‐Dimethyl‐2‐isopropenyl‐oxazolon‐(5)(DIO) wurden mit Diaminen unterschiedlicher Kettenlänge vernetzt. Aus Torsionsschwingungsversuchen ist zu erkennen, daß je nach DIO‐Anteil und Netzbrückenlänge mehr oder weniger uneinheitliche Netzwerke erhalten werden. Die Heterogenität ist zunächst einmal abhängig vom Copolymerisationsverhalten der Monomeren; DIO wird bevorzugt eingebaut, was schon bei ca. 15 mol‐% DIO zu einer signifikanten Heterogenität führt. Dieser Effekt ist bei Poly‐MA‐co‐DIO stärker ausgeprägt als bei Poly‐HMA‐co‐DIO. Einen weiteren Beitrag zur Heterogenität liefert die intramolekulare Vernetzung, die (infolge Zyklisierung) zu einer Kettenversteifung führt. Obwohl sowohl der Zyklisierungsgrad am Gelpunkt als auch die mechanisch wirksame Vernetzungsausbeute in ähnlicher Weise von der Kettenlänge des Diamins abhängen, besteht nur ein bedingter Zusammenhang zwischen diesen beiden Größen. Längerkettige Diamine führen einerseits zu einheitlicheren Netzwerken, weil sie weniger zur intramolekularen Vernetzung neigen, andererseits zu uneinheitlicheren, weil die Netzbrücken selbst nicht mehr mit dem Copolymerisat verträglich sind.
Recommendations for Automatic Responses to Electronic Mail (2004)

This memo makes recommendations for software that automatically responds to incoming electronic mail messages, including "out of the office" or "vacation" response generators, mail filtering software, email-based information services, and other automatic responders. The purpose of these recommendations is to discourage undesirable behavior which is caused or aggravated by such software, to encourage uniform behavior (where appropriate) among automatic mail responders, and to clear up some sources of confusion among implementors of automatic email responders.
Temperature-sensitive yeast mutants deficient in asparagine-linked glycosylation. (1982)

A [3H]mannose suicide selection has been used to isolate mutants in yeast which contain temperature-sensitive defects in asparagine-linked glycosylation. The surviving cells were screened at the nonpermissive temperature for a decreased ability to incorporate [3H]mannose and for defects in glycosylation of the secreted protein invertase. One of these mutants (alg1-1) has been characterized and found to be blocked in the assembly of the lipid-linked oligosaccharide precursor. The alg1-1 cells synthesize mannosyl compounds at 60% of the wild type level at the nonpermissive temperature and 105% of the wild type level at the permissive temperature. In vivo labeling experiments have demonstrated that alg1-1 cells are able to synthesize GlcNAc2-lipid but are unable to synthesize any mannose-containing oligosaccharide-lipids. This result was confirmed by in vitro labeling of yeast membranes. When incubated with UDP-[3H]GlcNAc, alg1-1 membranes synthesized GlcNAc2-lipid but failed to elongate it when GDP-Man was added. The alg1-1 membranes also failed to elongate exogenous GlcNAc2-lipid but were able to convert Man1GlcNAc2-lipid to Man5-Glc-NAc2-lipid in the presence of GDP-Man. These results indicate that the alg1-1 mutant is blocked specifically in the addition of the first mannose residue to the lipid-linked oligosaccharide precursor.
Data Security Through Qr Code Encryption And Steganography (2016)

The art of information hiding has become an important issue in the recent years as security of information has become a big concern in this internet era. Cryptography and Steganography play major role for secured data transfer. Steganography stands for concealed writing; it hides the message inside a cover medium. Cryptography conceals the content of a message by encryption. QR (Quick Response) Codes are 2-dimensional bar codes that encode text strings. They are able to encode information in both vertical and horizontal direction, thus able to encode more information. In this paper a novel approach is proposed for secret communication by combining the concepts of Steganography and QR codes. The suggested method includes two phases: (i) Encrypting the message by a QR code encoder and thus creating a QR code (ii) Hiding the QR code inside a colour image. This hiding process embeds the quantised QR code so that it will not make any visible distortion in the cover image and it introduces very minimum Bit Error Rate (BER). Experimental result shows that the proposed method has high imperceptibility, integrity and security..
Avoidance and management of peri-wound maceration of the skin. (2002)

Excessive wound exudate or other bodily fluids, such as urine or sweat, can cause skin maceration to occur around a wound, which may delay healing and lead to other complications. This paper examines means of preventing maceration while ensuring a moist wound-healing environment and looks at management techniques if the condition develops.
The representation of auditory source characteristics: Simple geometric form (1996)

Two experiments examined listeners’ ability to discriminate the geometric shape of simple resonating bodies on the basis of their corresponding auditory attributes. In cross-modal matching tasks, subjects listened to recordings of pairs of metal bars (experiment 1) or wooden bars (experiment 2) struck in sequence, and then selected a visual depiction of the bar cross sections that correctly represented their relative widths and heights from two opposing pairs presented on a computer screen. Multidimensional scaling solutions derived from matching scores for metal and wooden bars indicated that subjects’ performance varied directly with increasing differences in the width–height ratios of both sets of bars. Subsequent acoustic analyses revealed that the frequency components from torsional vibrational modes and the ratios of frequencies of transverse bending modes in the bars correlated strongly with both of the bars’ width–height ratios and bar coordinates in the multidimensional configurations. The results suggest that listeners can encode the auditory properties of sound sources by extracting certain invariant physical characteristics of their gross geometric properties from their acoustic behavior. [Work supported by CNRS.]
Learning long-term dependencies is not as difficult with NARX recurrent neural networks (1995)

It has recently been shown that gradient descent learning algorithms for recurrent neural networks can perform poorly on tasks that involve long--term dependencies, i.e. those problems for which the desired output depends on inputs presented at times far in the past. In this paper we explore the long--term dependencies problem for a class of architectures called NARX recurrent neural networks, which have powerful representational capabilities. We have previously reported that gradient descent learning is more effective in NARX networks than in recurrent neural network architectures that have &quot;hidden states&quot; on problems including grammatical inference and nonlinear system identification. Typically, the network converges much faster and generalizes better than other networks. The results in this paper are an attempt to explain this phenomenon. We present some experimental results which show that NARX networks can often retain information for two to three times as long as conventional rec...
Multiscale Hybrid Micro‐Nanocomposites Based on Carbon Nanotubes and Carbon Fibers (2010)

Amino‐modified double wall carbon nanotube (DWCNT‐NH 2 )/carbon fiber (CF)/epoxy hybrid micro‐nanocomposite laminates were prepared by a resin infusion technique. DWCNT‐NH 2 /epoxy nanocomposites and carbon fiber/epoxy microcomposites were made for comparison. Morphological analysis of the hybrid composites was performed using field emission scanning electron microscope. A good dispersion at low loadings of carbon nanotubes (CNTs) in epoxy matrix was achieved by a bath ultrasonication method. Mechanical characterization of the hybrid micro‐nanocomposites manufactured by a resin infusion process included three‐point bending, mode I interlaminar toughness, dynamic mechanical analysis, and drop‐weight impact testing. The addition of small amounts of CNTs (0.025, 0.05, and 0.1 wt%) to epoxy resins for the fabrication of multiscale carbon fiber composites resulted in a maximum enhancement in flexural modulus by 35%, a 5% improvement in flexural strength, a 6% improvement in absorbed impact energy, and 23% decrease in the mode I interlaminar toughness. Hybridization of carbon fiber‐reinforced epoxy using CNTs resulted in a reduction in T g and dampening characteristics, presumably as a result of the presence of micron‐sized agglomerates.
Exploring Security Issues and Solutions in Cloud Computing Services – A Survey (2017)

Abstract Cloud computing is emerging as one of the powerful computing technologies in the field of Information Technology due to its flexibility and cost reduction. This paper provides a detailed survey on security issues of the services provided by cloud computing and solutions to mitigate them. The main objective of this paper is to empower a new researcher to figure out the concepts of cloud computing, the services provided by them, and the security issues in the services. It also provides solutions to avoid or mitigate the different security issues which occur in the services provided by cloud computing. Additionally, it provides insight into the cloud computing model proposed by the National Institute of Standards and Technology (NIST), data stages and data security basics in a multi-tenant environment. This paper explores the different security methods proposed by different researchers and analyzes them.
Notes and News (1949)

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Web-based knowledge management (1998)

Despite the tremendous penetration of the Web into a variety of markets, it has become increasingly clear that current Web structures are simply not well designed for customized information access. By creating CGI scripts, plug-ins, and gateways, and bolting all of it onto the Web in an ad hoc fashion, we have moved away from what could have been a carefully planned model for customized information access. If we implement changes now, before the current technology creates difficult-to-handle legacy structures, we might be able to make the Web a lot more useful. The author discusses the use of metatags, information customization and automated linking.
Determination of the content of polysaccharide in Mycoblastus alpinus by UV spectrometry. (2012)

Objective To establish a method for determining the content of polysaccharide in Mycoblastus alpinus.Methods The content of polysaccharide in Mycoblastus alpinus was determined by ultraviolet spectrometry.Results The standard curve was of good linearity at the range of 20.846-104.230 μg·mL-1,r=0.997 0,and the average recovery was 106.4% with RSD 2.6%(n=6).Conclusion The method constructed was simple,repeatable and could be used to determine the content of polysaccharide in Mycoblastus alpinus effectively.
Gene finding in novel genomes (2004)

Since gene prediction is sensitive to species-specific parameters, every genome needs a dedicated gene finder.
A new analytical model of ultimate water cut for light oil reservoirs with bottom-wate (2018)

Ultimate water cut (WCult) defines well's maximum water production for uncontained oil pay with bottom-water. The WCult is important to determine if the reservoir development is economical. Since presently-used WCult formula derives from simplifying assumption ignoring the effect of non-radial inflow, the formula needs to be redefined. A new analytical formula of WCult is developed by considering the inflow of oil and water into separate completions at the top of oil-zone and aquifer respectively. Then the formula is verified using the design of 46 simulated experiments representing wide variety of reservoir-bottomwater systems. It was found out that the for light-oil reservoirs, the presently-used theoretical formula may significantly diverge from the proposed formula which closely matches the simulated data and is more physics driven. Hence the proposed formula should be preferred. However, for the viscous oil reservoirs, the presently used formula conforms to the proposed formula, which is also proved mathematically.
Research on heterocyclic compounds. XXXIII--Synthesis and analgesic activity of imidazo[1,2-b]pyridazine-2-acetic acid derivatives. (1995)

A series of imidazo[1,2-b]pyridazine-2-acetic esters, acids and amides was synthesized and tested for antiinflammatory, analgesic and ulcerogenic activities. The ethyl esters were prepared by cyclocondensation of some 3-aminopyridazines with ethyl 4-chloroacetoacetate, followed by hydrolysis or ammonolysis in order to obtain the corresponding acids and amides. The capacity of inhibiting the carrageenan-induced edema in the rat paw and the writhes induced by acetic acid in mice were evaluated, as well as the ulcerogenic action in rats. The acidic derivatives showed significant analgesic activity which is comparable to that found in other series of imidazo[1,2-b]pyridazine analogs previously examined.
A Study Of The Food Habits Of Large-Mouth Black Bass Fingerlings (Huro salmoides) In Rearing Ponds (1942)

Although studies have been made on the feeding-habits of bass-fingerlings in northern and eastern parts of the United States, no studies seem to have been published in the Southwest. Differences, both qualitative and quantitative, have been found between the plankters produced in the Southwest and in northern waters; and it is to be expected that these differences are reflected in the diet of fingerling fish.
Wood resource in Aspen: Ecology and Management in the Western United States (1985)

Aspen has not been cut extensively in the West; in fact, it has been grossly underutilized. For example, as recently as 1975, the aspen harvest from National Forests in four Forest Service regions in the Rocky Mountain area was 7.64 million board feet.1 Additional minor volumes were cut on special-use permits for products such as fuel and corral poles. The total amount cut represented only 0.1% of the net volume available in these aspen forests.
HPLC−CD On-Line Coupling in Combination with HPLC−NMR and HPLC−MS/MS for the Determination of the Full Absolute Stereostructure of New Metabolites in Plant Extracts (1999)

The applicability of on-line coupling of gradient eluted nonchiral reversed-phase high-pressure liquid chromatography (HPLC) to circular dichroism (CD) spectroscopy for the stereochemical analysis of crude plant extracts was investigated. A detector measuring simultaneously UV absorbances and CD effects at constant wavelengths in the same detection cell was used. The solvent system water/acetonitrile was found to be suited to give high-quality CD chromatograms as a function of the elution time and even complete characteristic CD spectra in the stop-flow mode. The method was applied to the tropical liana Habropetalum dawei (Dioncophyllaceae). First, the extract solution was investigated by the combined application of HPLC−NMR and HPLC−ESI-MS/MS techniques. The constitution and relative configuration of two as yet unknown natural products thus detected, the isoquinoline phylline (5) and the naphthylisoquinoline alkaloid 5‘-O-methyldioncopeltine A (6), were characterized. Subsequent HPLC−CD experiments permitted the deduction of the absolute configurations of these new metabolites by empirical analysis of their CD data. Thus, using identical sample solutions and chromatographic conditions (including the same columns and solvent gradient programs), the combination of NMR, MS, and CD data permitted the complete structural elucidation of the two new metabolites in the extract matrix solution, without any isolation and sample purification prior to the coupling experiments.
Erratum: Noninvasive sheet charge density probe for integrated silicon devices [Appl. Phys. Lett. <b>4</b> <b>8</b>, 1066 (1986)] (1986)

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On-line shelf-life prediction in perishable goods chain through the integration of WSN technology with a 1st order kinetic model (2015)

The improvements in sensors and wireless technology offer an effective way to enhance food safety and certification along all the perishable goods supply-chain, in order to reduce food waste and losses, while guaranteeing a high degree of quality and preventing diseases directly related to the use of expired or harmful products. In this paper, a complete system for continuous environmental parameters (i.e. temperature, light exposition and relative humidity) acquisition and real-time shelf-life prediction of monitored product is proposed. An algorithm based on a 1 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">st</sup> order kinetic model of the product quality decay with a variation rate evaluated accordingly to the Arrhenius law is proposed. A case study is also shown, i.e.: data during the storage phase of agricultural product (tomatoes) have been acquired through a wireless sensor networks and uploaded to a cloud service. The collected data, a sample per 15 minutes, are processed by the computation algorithm implemented on laptop: the overall delay due to data download and processing is just about 0,3 s. As consequence, the remaining shelf-life of the food can be estimated with a 5% uncertainty with a 2K temperature sensor, highlighting critical situation in the manufacturing environment and allowing timely intervention.
Disruption of MyD88 signaling suppresses hemophagocytic lymphohistiocytosis in mice (2011)

Hemophagocytic lymphohistiocytosis (HLH) is a rare inflammatory disorder with a poor prognosis for affected individuals. To find a means of suppressing the clinical phenotype, we investigated the cellular and molecular mechanisms leading to HLH in Unc13d(jinx/jinx) mice, in which cytolytic function of NK and CD8(+) T cells is impaired. Unc13d(jinx/jinx) mutants infected with lymphochoriomeningitis virus (LCMV) present typical clinical features of HLH, including splenomegaly, elevated serum IFNγ, and anemia. Proteins mediating cell-cell contact, cytokine signaling or Toll-like receptor (TLR) signaling were analyzed. We show that neither the integrin CD18, which is involved in adhesion between antigen-presenting cells and effector T cells, nor tumor necrosis factor (TNF) made nonredundant contributions to the disease phenotype. Disruption of IFNγ signaling reduced immune cell activation in Unc13d(jinx/jinx) mice, but also resulted in uncontrolled viral proliferation and exaggerated release of inflammatory cytokines. Abrogating the function of myeloid differentiation primary response gene 88 (MyD88) in Unc13d(jinx/jinx) mice suppressed immune cell activation and controlled cytokine production in an IL-1 receptor 1 (IL-1R1)-independent way. Our findings implicate MyD88 as the key initiator of myeloid and lymphoid proliferation in HLH, and suggest that blockade of this signaling molecule may reduce immunopathology in patients.
Pharmacodynamic-mediated reduction of temozolomide tumor concentrations by the angiogenesis inhibitor TNP-470. (2001)

The angiogenic phenotype is associated with increased tumor neovascularization and a state of vascular hyperpermeability to macromolecules. Angiogenesis inhibitors could reverse these processes, resulting in tumor capillaries that have normal membrane permeability. It was proposed that the switch from a hyperpermeable to a normal permeable state could have the untoward effect of decreasing tumor concentrations of anticancer drugs coadministered with angiogenesis inhibitors. The current investigation evaluated a potential drug interaction between the angiogenesis inhibitor O-(N-chloroacetyl-carbamoyl)-fumagillol (TNP-470) and the alkylating agent temozolomide (TMZ), in xenograft models that differentially expressed vascular endothelial growth factor (VEGF), a driving force for angiogenesis. Nude rats bearing either s.c. low VEGF (V-) or high VEGF (V+) or intracerebral V+ gliomas were administered either a multiple-dose regimen of TNP-470 or vehicle control. One day after the last dose of vehicle or TNP-470, a steady-state dosing regimen of TMZ was administered with subsequent collection and high-performance liquid chromatography analysis of plasma and either tumor homogenate or tumor microdialysis steady-state TMZ concentrations, and in some cases [5-(3-methyltriazen-1-yl)imidazole-4-carboximide] MTIC, its active metabolite. Microvessel density (MVD) was quantitated by image analysis using an anti-CD31 method. Statistical analyses of pharmacokinetic and pharmacodynamic end points in the control and TNP-470 treatment groups were completed by nonparametric tests. In both the s.c. and intracerebral V+ models, TNP-470 treatment produced significant reductions in TMZ tumor concentrations and tumor:plasma concentration ratios compared with control, being reduced an average of 25% and 50% in the s.c. and intracerebral tumors, respectively. MTIC concentrations in V+ s.c. tumors also were reduced by 50% in the presence of TNP-470. Consistent with the lower extent of neovascularization in the V- tumors, TMZ and MTIC tumor concentrations were not different in TNP-470 and control treatment groups in s.c. tumors. MVD was reduced by TNP-470 compared with vehicle control in the V+ tumors, but was unaltered in V- tumors, attesting to the use of MVD as a pharmacodynamic end point and the effectiveness of TNP-470 as an angiogenesis inhibitor. Angiogenesis inhibitor's pharmacodynamic actions on tumor angiogenesis can produce a reduction in tumor concentrations of coadministered anticancer agents. It is increasingly important to understand the pharmacokinetic and pharmacodynamic behavior of each class of drug so that optimal dosing regimens can be designed.
The ASYST Software for Scientific Computing (1987)

The acceptance of ASYST by the scientific community could dramatically change the way scientific data are handled and reduce the need for extensive in-house software development for many applications. However, there is no substitute for a well-conceived use of any software. We feel that the full acceptance of the ideas and concepts pioneered by Adaptable Laboratory Software and other software houses will depend on the ability of the scientific community to fully test and verify the procedures used by such products. Only then can the results produced by these software packages be subjected to confirmation which is crucial to rigorous scientific endeavor.