gebhart/patrepeval huggingface dataset:
Salinity Constraints on Subsurface Archaeal Diversity and Methanogenesis in Sedimentary Rock Rich in Organic Matter (2007)

ABSTRACT The diversity of microorganisms active within sedimentary rocks provides important controls on the geochemistry of many subsurface environments. In particular, biodegradation of organic matter in sedimentary rocks contributes to the biogeochemical cycling of carbon and other elements and strongly impacts the recovery and quality of fossil fuel resources. In this study, archaeal diversity was investigated along a salinity gradient spanning 8 to 3,490 mM Cl − in a subsurface shale rich in CH 4 derived from biodegradation of sedimentary hydrocarbons. Shale pore waters collected from wells in the main CH 4 -producing zone lacked electron acceptors such as O 2 , NO 3 − , Fe 3+ , or SO 4 2− . Acetate was detected only in high-salinity waters, suggesting that acetoclastic methanogenesis is inhibited at Cl − concentrations above ∼1,000 mM. Most-probable-number series revealed differences in methanogen substrate utilization (acetate, trimethylamine, or H 2 /CO 2 ) associated with chlorinity. The greatest methane production in enrichment cultures was observed for incubations with salinity at or close to the native pore water salinity of the inoculum. Restriction fragment length polymorphism analyses of archaeal 16S rRNA genes from seven wells indicated that there were links between archaeal communities and pore water salinity. Archaeal clone libraries constructed from sequences from 16S rRNA genes isolated from two wells revealed phylotypes similar to a halophilic methylotrophic Methanohalophilus species and a hydrogenotrophic Methanoplanus species at high salinity and a single phylotype closely related to Methanocorpusculum bavaricum at low salinity. These results show that several distinct communities of methanogens persist in this subsurface, CH 4 -producing environment and that each community is adapted to particular conditions of salinity and preferential substrate use and each community induces distinct geochemical signatures in shale formation waters.
Pharmacogenomics of gemcitabine: can genetic studies lead to tailor-made therapy? (2007)

Gemcitabine is a deoxycytidine analogue that has a broad spectrum of antitumour activity in many solid tumours including pancreatic cancer. We have recently carried out a pharmacogenomic study in cancer patients treated with gemcitabine, and found that one genetic polymorphism of an enzyme involved in gemcitabine metabolism can cause interindividual variations in the pharmacokinetics and toxicity of this agent. In this paper, we review recent genetic studies of gemcitabine, and discuss the possibility of individualised cancer chemotherapy based on a pharmacogenomic approach.
THE DIAGNOSIS OF SILICOSIS, WITH SPECIAL REFERENCE TO ROENTGENOLOGICAL MANIFESTATIONS (1936)

Article1 August 1936THE DIAGNOSIS OF SILICOSIS, WITH SPECIAL REFERENCE TO ROENTGENOLOGICAL MANIFESTATIONSLEROY U. GARDNER, M.D.LEROY U. GARDNER, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-10-2-166 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptBecause conditions caused by the inhalation of dust are so frequently involved in litigation it is necessary that they be defined with the greatest clarity. Our terminology developed forty to fifty years ago, before the pathology of such conditions had been worked out. Pneumoconiosis was the term coined to designate disease of the lungs due to inhaled dust, regardless of its nature. Anthracosis, siderosis, chalicosis, silicosis were specific forms of pneumoconiosis due to inhaled coal, iron, stone dust and silica respectively. All were considered of equal significance as it was believed that any dust was merely a foreign body which,...References1. MEREWETHER ER: Memorandum on asbestosis, Tubercle, 1934, xv, 69-81, 109. Google Scholar2. GLOYNE SR: Effect of inhalation of asbestos dust on lungs of asbestos workers, Tubercle, 1932-33, xiv, 445, 493, 450. Google Scholar3. LANZAMCCONNELLFEHNEL AJWJJW: Morbid anatomy and histology of asbestosis, U. S. Pub. Health Rep., 1935, l, 1-12. CrossrefGoogle Scholar This content is PDF only. To continue reading please click on the PDF icon. Author, Article, and Disclosure InformationAffiliations: Saranac Lake, N. Y.*Read at the Detroit meeting of the American College of Physicians, March 5, 1936. PreviousarticleNextarticle Advertisement FiguresReferencesRelatedDetails Metrics 1 August 1936Volume 10, Issue 2Page: 166-173KeywordsDustInhalationLungsPneumoconiosesSilicosis ePublished: 1 December 2008 Issue Published: 1 August 1936 PDF downloadLoading ...
Healing of Large Defects Treated With Calcium Sulfate Pellets Containing Demineralized Bone Matrix Particles (2003)

Calcium sulfate (OsteoSet, Wright Medical Technology, Inc, Arlington, Tenn) and calcium sulfate/demineralized bone matrix (DBM) pellets (OsteoSet DBM, Wright Medical Technology, Inc) have been evaluated preclinically in a bilateral medullary defect model of a canine humerus. In this model, both short (6 week) and long (26 week) time points have been evaluated. An analysis of bone response to the pellets was conducted using radiological, histological, mechanical, and quantification techniques. The calcium sulfate/DBM pellets exhibited more rapid trabecular bone remodeling as demonstrated by the absence of the ringlet bone structure typically seen with calcium sulfate pellets. We concluded that calcium sulfate and calcium sulfate/DBM pellets are both effective bone graft substitutes.
Wound Healing Bionanocomposites Based on Castor Oil Polymeric Films Reinforced with Chitosan-Modified ZnO Nanoparticles (2015)

Castor oil (CO), which is a readily available, relatively inexpensive, and environmentally benign nonedible oil, has been successfully used as matrix material to prepare biocompatible and biodegradable nanocomposite films filled with chitosan (CS)-modified ZnO nanoparticles. The biocomposites were synthesized via a simple and versatile solution mixing and casting method. The morphology, structure, thermal stability, water absorption, biodegradability, cytocompatibility, barrier, mechanical, viscoelastic, antibacterial, and wound healing properties of the films have been analyzed. FT-IR spectra were used to obtain information about the nanoparticle-matrix interactions. The thermal stability, hydrophilicity, degree of porosity, water absorption, water vapor transmission rate (WVTR), oxygen permeability (Dk), and biodegradability of the films increased with the CS-ZnO loading. The WVTR and Dk data obtained are within the range of values reported for commercial wound dressings. Tensile tests demonstrated that the nanocomposites displayed a good balance between elasticity, strength, and flexibility under both dry and simulated body fluid (SBF) environments. The flexibility increased in a moist atmosphere due to the plasticization effect of absorbed water. The nanocomposites also exhibited significantly enhanced dynamic mechanical performance (storage modulus and glass transition temperature) than neat CO under different humidity conditions. The antibacterial activity of the films against Escherichia coli, Staphylococcus aureus, and Micrococcus luteus bacteria was investigated in the presence and the absence of UV light. The biocide effect increased progressively with the CS-ZnO content and was systematically stronger against Gram-positive cells. Composites with nanoparticle loading ≤5.0 wt % exhibited very good in vitro cytocompatibility and enabled a faster wound healing than neat CO and control gauze, hence showing great potential to be applied as antibacterial wound dressings.
Effect of Chemical and Microbial Amendments on Ammonia Volatilization from Composting Poultry Litter (2004)

Research has shown that aluminum sulfate (alum) and phosphoric acid greatly reduce ammonia (NH3) volatilization from poultry litter; however, no studies have yet reported the effects of these amendments on field-scale composting of poultry litter. The objectives of this study were to (i) evaluate NH3 volatilization from composting litter by measuring both NH3 volatilization and changes in total nitrogen (N) in the litter and (ii) evaluate potential methods of reducing NH3 losses from composting poultry litter. Poultry litter was composted for 68 d the first year and 92 d the second year. Eleven treatments were screened in Year 1, which included an unamended control, a microbial mixture, a microbial mixture with 5% alum incorporated into the litter, 5 and 10% alum rates either surface-applied or incorporated, and 1 and 2% phosphoric acid rates either surface-applied or incorporated. Treatments in Year 2 included an unamended control, a microbial mixture, alum (7% by fresh wt.), and phosphoric acid (1.5% by fresh wt.). Alum and phosphoric acid reduced NH3 volatilization from composting poultry litter by as much as 76 and 54%, respectively. The highest NH3 emission rates were from microbial treatments each year. Compost treated with chemical amendments retained more initial N than all other treatments. Due to the cost and N loss associated with composting poultry litter, composting is not economical from an agronomic perspective compared with the use of fresh poultry litter.
p-Cymene Studies. VI. Vapor Pressure of p-Cymene, Some of its Derivatives and Related Compounds (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTp-Cymene Studies. VI. Vapor Pressure of p-Cymene, Some of its Derivatives and Related CompoundsKenneth A. Kobe, Thomas S. Okabe, Marshall T. Ramstad, and Philip M. HuemmerCite this: J. Am. Chem. Soc. 1941, 63, 12, 3251–3252Publication Date (Print):December 1, 1941Publication History Published online1 May 2002Published inissue 1 December 1941https://pubs.acs.org/doi/10.1021/ja01857a004https://doi.org/10.1021/ja01857a004research-articleACS PublicationsRequest reuse permissionsArticle Views269Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Role of β-Adrenergic Receptors in the Oral Activity of Zinc-α2-Glycoprotein (ZAG) (2012)

Zinc-α2-glycoprotein (ZAG) is an adipokine with the potential as a therapeutic agent in the treatment of obesity and type 2 diabetes. In this study we show that human ZAG, which is a 41-kDa protein, when administered to ob/ob mice at 50 μg/d(-1) orally in the drinking water produced a progressive loss of body weight (5 g after 8 d treatment), together with a 0.5 C increase in rectal temperature and a 40% reduction in urinary excretion of glucose. There was also a 33% reduction in the area under the curve during an oral glucose tolerance test and an increased sensitivity to insulin. These results were similar to those after iv administration of ZAG. However, tryptic digestion was shown to inactivate ZAG. There was no evidence of human ZAG in the serum but a 2-fold elevation of murine ZAG, which was also observed in target tissues such as white adipose tissue. To determine whether the effect was due to interaction of the human ZAG with the β-adrenergic (β-AR) in the gastrointestinal tract before digestion, ZAG was coadministered to ob/ob mice together with propanolol (40 mg/kg(-1)), a nonspecific β-AR antagonist. The effect of ZAG on body weight, rectal temperature, urinary glucose excretion, improvement in glucose disposal, and increased insulin sensitivity were attenuated by propanolol, as was the increase in murine ZAG in the serum. These results suggest that oral administration of ZAG increases serum levels through interaction with a β-AR in the upper gastrointestinal tract, and gene expression studies showed this to be in the esophagus.
Electronic damping of orthogonal bending modes in a cylindrical mast - Theory (1981)

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.
Distinct mechanisms of extrathymic T cell tolerance due to differential expression of self antigen (1992)

Self-reactive T lymphocytes escaping thymic tolerance induction can be rendered non-responsive by contact with antigens in the periphery. In order to determine the parameters controlling peripheral tolerance induction we followed the fate of one well-defined self-reactive T cell population in three different mice expressing the self antigen in various nonlymphoid tissues outside the thymus. This was achieved by crossing anti-Kb T cell receptor (TCR) transgenic mice with transgenic animals expressing the Kb antigen exclusively on hepatocytes or keratinocytes or neuroectodermal cells. Due to this differential expression clonotype+, anti-Kb reactive T cells were found to exist at three different levels of tolerance. These levels were distinct with regard to downregulation of TCR and CD8 molecules, and the requirements for reexpression of TCR in vitro. This differential induction of peripheral tolerance suggest that some tissues are more likely to be affected in autoimmune diseases than others.
Coherent detection imaging for laser-absorption-computed tomography (1991)

We present the first demonstration of computerassisted laser absorption tomography of actual biological objects that use the coherent detection imaging (CDI) method. This method is based on the optical heterodyne detection technique for directional resolution and image reconstruction by using the projection slice theorem from sets of line integrals of laser absorption along a large number of rays crossing the object with parallel-beam geometry.1,2
Neuroprotective effects of metabotropic glutamate receptor ligands in a 6‐hydroxydopamine rodent model of Parkinson's disease (2005)

Increasing evidence implicates glutamate-mediated excitotoxicity as a contributory factor in dopaminergic cell death in the substantia nigra pars compacta (SNc) in Parkinson's disease (PD). Previous studies have suggested that metabotropic glutamate receptor (mGluR) ligands are neuroprotective against excitotoxicity in vitro. In the present study, the neurotoxin 6-hydroxydopamine (6-OHDA) produced a significant loss (61.2 +/- 8.9%; P < 0.01) of tyrosine hydroxylase-immunopositive (TH+) cells in both the SNc and striatal dopamine (58.02 +/- 1.27%; P < 0.05) in control male Sprague-Dawley rats. Both losses were significantly attenuated by sub-chronic (7 day) treatment with the Group I mGluR antagonists, 2-methyl-6(phenylethynyl)-pyridine (MPEP) or (S)-(+)-alpha-amino-4-carboxy-2-methylbenzeneacetic acid (LY367385); the Group II mGluR agonist (2R,4R)-4-aminopyrrolidine-2,4-dicarboxylate (2R,4R-APDC); or the Group III mGluR agonist, L(+)-2-amino-4-phosphonobutyric acid (L-AP4). These data demonstrate a neuroprotective action of mGluR ligands in vivo against 6-OHDA toxicity that has important implications for the treatment of PD.
Isolation and Characterization of a Novel Calmodulin-binding Protein from Potato (2002)

Tuberization in potato is controlled by hormonal and environmental signals. Ca(2+), an important intracellular messenger, and calmodulin (CaM), one of the primary Ca(2+) sensors, have been implicated in controlling diverse cellular processes in plants including tuberization. The regulation of cellular processes by CaM involves its interaction with other proteins. To understand the role of Ca(2+)/CaM in tuberization, we have screened an expression library prepared from developing tubers with biotinylated CaM. This screening resulted in isolation of a cDNA encoding a novel CaM-binding protein (potato calmodulin-binding protein (PCBP)). Ca(2+)-dependent binding of the cDNA-encoded protein to CaM is confirmed by (35)S-labeled CaM. The full-length cDNA is 5 kb long and encodes a protein of 1309 amino acids. The deduced amino acid sequence showed significant similarity with a hypothetical protein from another plant, Arabidopsis. However, no homologs of PCBP are found in nonplant systems, suggesting that it is likely to be specific to plants. Using truncated versions of the protein and a synthetic peptide in CaM binding assays we mapped the CaM-binding region to a 20-amino acid stretch (residues 1216-1237). The bacterially expressed protein containing the CaM-binding domain interacted with three CaM isoforms (CaM2, CaM4, and CaM6). PCBP is encoded by a single gene and is expressed differentially in the tissues tested. The expression of CaM, PCBP, and another CaM-binding protein is similar in different tissues and organs. The predicted protein contained seven putative nuclear localization signals and several strong PEST motifs. Fusion of the N-terminal region of the protein containing six of the seven nuclear localization signals to the reporter gene beta-glucuronidase targeted the reporter gene to the nucleus, suggesting a nuclear role for PCBP.
Single Wafer Furnace and Its Thermal Processing Applications (2000)

A resistively heated, vacuum and atmospheric pressure compatible, single wafer furnace (SWF) system is proposed to improve operational flexibility of conventional furnaces and productivity of single wafer rapid thermal processing (RTP) systems. The design concept and hardware configuration of the SWF system are described. The temperature measurement/control techniques and thermal characteristics of the SWF system are described. Typical process results in TiSi formation, implant anneal and thin oxide formation using the SWF system are reported. Due to the vertically stacked, dual chamber configuration and steady state temperature control, very flexible operation with a high throughput at a minimal power consumption (<3.5 kW per process chamber at 1150°C) was realized. Many thermal processes used in furnaces and RTP systems can easily be converted to SWF processes without decreasing cost performance and/or deteriorating process results by using the SWF system.
Identification of Pulmonary T-Lymphocyte and Serum Antibody Isotype Responses Associated with Protection against<i>Rhodococcus equi</i> (2002)

Rhodococcus equi infects and causes pneumonia in foals between 2 and 4 months of age but does not induce disease in immunocompetent adults, which are immune and remain clinically normal upon challenge. Understanding the protective response against R. equi in adult horses is important in the development of vaccine strategies, since those mechanisms likely reflect the protective phenotype that an effective vaccine would generate in the foal. Twelve adult horses were challenged with virulent R. equi and shown to be protected against clinical disease. Stimulation of cells obtained from bronchoalveolar lavage fluid with either R. equi or the vaccine candidate protein VapA resulted in significant proliferation and a significant increase in the level of gamma interferon (IFN-gamma) expression by day 7 postchallenge. The levels of interleukin-4 expression were also increased at day 7 postchallenge; however, this increase was not antigen specific. Anamnestic increases in the levels of binding to R. equi and VapA of all immunoglobulin G (IgG) antibody isotypes [IgGa, IgGb, IgG(T)] examined were detected postchallenge. The levels of R. equi- and VapA-specific IgGa and IgGb antibodies, the IgG isotypes that preferentially opsonize and fix complement in horses, were dramatically enhanced postchallenge. The antigen-specific proliferation of bronchoalveolar lavage fluid cells, the levels of IFN-gamma expression by these cells, and the anamnestic increases in the levels of opsonizing IgG isotypes are consistent with stimulation of a memory response in immune adult horses and represent correlates for vaccine development in foals.
Electromagnetics in South Africa: Perspectives and Recent Achievements (2006)

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Membrane-Bound and Exosomal Metastasis-Associated C4.4A Promotes Migration by Associating with the α6β4 Integrin and MT1-MMP (2012)

Metastasis-associated C4.4A, which becomes upregulated during wound healing and, in some tumors, during tumor progression, is known to be frequently associated with hypoxia. With the function of C4.4A still unknown, we explored the impact of hypoxia on C4.4A expression and functional activity. Metastatic rat and human tumor lines upregulate C4.4A expression when cultured in the presence of CoCl(2). Although hypoxia-inducible factor 1α (HIF-1α) becomes upregulated concomitantly, HIF-1α did not induce C4.4A transcription. Instead, hypoxia-induced C4.4A up-regulation promoted in vivo and in vitro wound healing, where increased migration on the C4.4A ligands laminin-111 and -332 was observed after a transient period of pronounced binding. Increased migration was accompanied by C4.4A associating with α(6)β(4), MT1-MMP1, and TACE and by laminin fragmentation. Hypoxia also promoted the release of C4.4A in exosomes and TACE-mediated C4.4A shedding. The association of C4.4A with α(6)β(4) and MT1-MMP1 was maintained in exosomes and exosomal α(6)β(4)- and MT1-MMP1-associated C4.4A but not shed C4.4A sufficient for laminin degradation. Hypoxia-induced recruitment of α(6)β(4) toward raft-located C4.4A, MT1-MMP, and TACE allows for a shift from adhesion to motility, which is supported by laminin degradation. These findings provide the first explanation for the C4.4A contribution to wound healing and metastasis.
Dynamic regulation of <i>Pdx1</i> enhancers by Foxa1 and Foxa2 is essential for pancreas development (2008)

The onset of pancreas development in the foregut endoderm is marked by activation of the homeobox gene Pdx1 (IPF1). Pdx1 is essential for the expansion of the pancreatic primordium and the development of endocrine islets. The control of Pdx1 expression has been only partially elucidated. We demonstrate here that the winged-helix transcription factors Foxa1 and Foxa2 co-occupy multiple regulatory domains in the Pdx1 gene. Compound conditional ablation of both Foxa1 and Foxa2 in the pancreatic primordium results in complete loss of Pdx1 expression and severe pancreatic hypoplasia. Mutant mice exhibit hyperglycemia with severely disrupted acinar and islet development, and die shortly after birth. Assessment of developmental markers in the mutant pancreas revealed a failure in the expansion of the pancreatic anlage, a blockage of exocrine and endocrine cell differentiation, and an arrest at the primitive duct stage. Comparing their relative developmental activity, we find that Foxa2 is the major regulator in promoting pancreas development and cell differentiation. Using chromatin immunoprecipitations (ChIP) and ChIP sequencing (ChIPSeq) of fetal pancreas and islet chromatin, we demonstrate that Foxa1 and Foxa2 predominantly occupy a distal enhancer at -6.4 kb relative to the transcriptional start site in the Pdx1 gene. In addition, occupancy of the well-characterized proximal Pdx1 enhancer by Foxa1 and Foxa2 is developmental stage-dependent. Thus, the regulation of Pdx1 expression by Foxa1 and Foxa2 is a key early event controlling the expansion and differentiation of the pancreatic primordia.