gebhart/patrepeval huggingface dataset:
Coordinate expression of multiple proteins in plant cells by exploiting endogenous kex2p‐like protease activity (2011)

Simultaneous expression of multiple proteins in plants finds ample applications. Here, we examined the biotechnological application of native kex2p-like protease activity in plants for coordinate expression of multiple secretory proteins from a single transgene encoding a cleavable polyprotein precursor. We expressed a secretory red fluorescent protein (DsRed) or human cytokine (GMCSF), fused to a downstream green fluorescent protein (GFP) by a linker containing putative recognition sites of the kex2p-like protease in tobacco cells and referred to them as RKG and GKG cells, respectively. Our analyses showed that GFP is cleaved off the fusion proteins and secreted into the media by both RKG and GKG cells. The cleaved GFP product displayed the expected fluorescence characteristics. Using GFP immunoprecipitation and fluorescence analysis, the cleaved DsRed product in the RKG cells was found to be functional as well. However, DsRed was not detected in the RKG culture medium, possibly due to its tetramer formation. Cleaved and biologically active GMCSF could also be detected in GKG cell extracts, but secreted GMCSF was found to be only at a low level, likely because of instability of GMCSF protein in the medium. Processing of polyprotein precursors was observed to be similarly effective in tobacco leaf, stem and root tissues. Importantly, we also demonstrated that, via agroinfiltration, polyprotein precursors can be efficiently processed in plant species other than tobacco. Collectively, our results demonstrate the utility of native kex2p-like protease activity for the expression of multiple secretory proteins in plant cells using cleavable polyprotein precursors containing kex2p linker(s).
Effect of adhesive antibiotic TA on plaque and gingivitis in man (1989)

The adhesive antibiotic TA was applied to the dento-gingival junction of 8 human volunteers, suffering from moderate to severe gingivitis. 2 diametrically opposed quadrants of the mouth received 4 applications of 0.1 mg TA, while the other 2 quadrants were treated with a placebo and served as controls. The plaque index, gingival index and bleeding index were scored periodically for 2 weeks and in 4 patients for up to 30 days. The TA-treated quadrants showed a rapid decrease in all 3 indices following 2 treatments. A further improvement was observed with the 3rd and 4th treatments. 30 days after the onset of the experiment and 23 days after the last application, the indices were still considerably lower than the initial values.
A New System for Continuous Monitoring of Breathing and Kinetic Activity (2010)

We present a new system for acquiring simultaneously breathing rate and kinetic activity over a period of twenty-four hours. The system is based on a couple of sensors, which are very light, absolutely noninvasive, and compatible with everyday life. The proposed breathing sensor is cheap and uses a conductive rubber as active material. An analog signal representing the breathing rate is obtained from the sensor's signal, breath by breath, without any averaging or filtering. The kinetic activity sensor is based on a tiny accelerometer whose signal is averaged and filtered, so that both sensors have voltage compatible with a slow data logging.
Moderate and high amounts of tamoxifen in <i>α-MHC-MerCreMer</i> mice induce a DNA damage response, leading to heart failure and death (2013)

Numerous mouse models have utilized Cre-loxP technology to modify gene expression. Adverse effects of Cre recombinase activity have been reported, including in the heart. However, the mechanisms associated with cardiac Cre toxicity are largely unknown. Here, we show that expression of Cre in cardiomyocytes induces a DNA damage response, resulting in cardiomyocyte apoptosis, cardiac fibrosis and cardiac dysfunction. In an effort to increase the recombination efficiency of a widely used tamoxifen-sensitive Cre transgene under control of the α-myosin-heavy-chain promoter (αMHC-MerCreMer), we observed myocardial dysfunction and decreased survival, which were dependent on the dose of tamoxifen injected. After excluding a Cre-independent contribution by tamoxifen, we found that Cre induced myocardial fibrosis, activation of pro-fibrotic genes and cardiomyocyte apoptosis. Examination of the molecular mechanisms showed activation of DNA damage response signaling and p53 stabilization in the absence of loxP sites, suggesting that Cre induced illegitimate DNA breaks. Cardiomyocyte apoptosis was also induced by expressing Cre using adenoviral transduction, indicating that the effect was not dependent on genomic integration of the transgene. Cre-mediated homologous recombination at loxP sites was dose-dependent and had a ceiling effect at ∼80% of cardiomyocytes showing recombination. By titrating the amount of tamoxifen to maximize recombination while minimizing animal lethality, we determined that 30 μg tamoxifen/g body weight/day injected on three consecutive days is the optimal condition for the αMHC-MerCreMer system to induce recombination in the Rosa26-lacZ strain. Our results further highlight the importance of experimental design, including the use of appropriate genetic controls for Cre expression.
Experimental Validation of INS Monitor against GNSS Spoofing (2018)

Global Navigation Satellite System (GNSS) spoofing attacks are a critical threat to navigation integrity. We previously developed an analytical framework to evaluate the performance of Inertial Navigation System (INS) monitors against spoofing attacks, which is necessary for eventual clarification and certification of the INS monitor. In this paper, we develop and execute an experimental setup to verify the resistance of the monitor against worstcase GNSS spoofing attacks. The test setup includes generation of the worst-case spoofed code and carrier phase GPS signals (using a GNSS simulator) that feed the receiver through a cable without needing to broadcast via transmitter. Using these signals, the miss-detection rates of the Kalman filter innovations-based monitor, has been observed through a tightly-coupled integration of a tactical grade inertial and a standalone GNSS receiver on a static platform. The experimental evaluation technique in this paper is fundamental because it is the first hardware test to quantify spoofing resistance of a widely used IMU with worst-case spoofing signals. We realistically demonstrate that, even if the spoofer tracks the position of the receiver and filters the high-frequency tracking errors, the monitor is still capable of detecting that with low missed detection probability.
A Microprocessor-Controlled High-Accuracy Wide-Range Speed Regulator for Motor Drives (1982)

A new speed measurement system, which is suitable for microprocessor-based motor drive, and its application to speed regulator are explained.
Low-cost flat antennas for mobile and wireless communications (2002)

A class of flat antenna arrays that uses multi-layer printed circuit technology offers several advantages that make them attractive for mobile and wireless communications. Lower profile and lighter weight are among the desirable features. This paper surveys the applications that may use this type of antenna and reviews some of the developments for consumer terminals in a number of mobile and wireless communications systems. The first example is for a Ku-band briefcase mobile terminal in a code-division multiple access (CDMA) system in which interference due to high sidelobes can be tolerated. Two realizations of the transmit/receive antenna are discussed. The second example is for a briefcase mobile terminal at S-band for the INSAT satellite system. The third example is at Ka-band where considerable interest exists for local multimedia distribution systems (LMDS) and a number of emerging satellite systems that are planned.
In Vitro Antibiotic Susceptibilities of Ocular Isolates in North and South America (1998)

The fluoroquinolones ofloxacin, ciprofloxacin, and norfloxacin had higher overall in vitro efficacy than the other antibiotics tested. Of the fluoroquinolones, ofloxacin had the highest in vitro efficacy against gram-positive organisms. All three fluoroquinolones were equivalent in efficacy against gram-negative organisms.
An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruses (2015)

Adeno-associated viruses (AAV) have evolved to exploit the dynamic reorganization of host cell machinery during co-infection by adenoviruses and other helper viruses. In the absence of helper viruses, host factors such as the proteasome and DNA damage response machinery have been shown to effectively inhibit AAV transduction by restricting processes ranging from nuclear entry to second-strand DNA synthesis. To identify host factors that might affect other key steps in AAV infection, we screened an siRNA library that revealed several candidate genes including the PHD finger-like domain protein 5A (PHF5A), a U2 snRNP-associated protein. Disruption of PHF5A expression selectively enhanced transgene expression from AAV by increasing transcript levels and appears to influence a step after second-strand synthesis in a serotype and cell type-independent manner. Genetic disruption of U2 snRNP and associated proteins, such as SF3B1 and U2AF1, also increased expression from AAV vector, suggesting the critical role of U2 snRNP spliceosome complex in this host-mediated restriction. Notably, adenoviral co-infection and U2 snRNP inhibition appeared to target a common pathway in increasing expression from AAV vectors. Moreover, pharmacological inhibition of U2 snRNP by meayamycin B, a potent SF3B1 inhibitor, substantially enhanced AAV vector transduction of clinically relevant cell types. Further analysis suggested that U2 snRNP proteins suppress AAV vector transgene expression through direct recognition of intact AAV capsids. In summary, we identify U2 snRNP and associated splicing factors, which are known to be affected during adenoviral infection, as novel host restriction factors that effectively limit AAV transgene expression. Concurrently, we postulate that pharmacological/genetic manipulation of components of the spliceosomal machinery might enable more effective gene transfer modalities with recombinant AAV vectors.
Effects of Cotton Variety Selection and Irrigation Level on Economic Returns: Texas Southern High Plains (2010)

As technology increases and new varieties are released for cotton production, producers have more options when choosing inputs and input levels. Studies have been conducted at the Agricultural Complex for Advanced Research and Extension Systems (AG-CARES) research facilities at Lamesa, Texas to determine the agronomic impacts of variety selection and applied irrigation level on cotton production. This study analyzes the effects of variety selection and irrigation level and allocation of limited irrigation water for irrigated cotton producers in the Texas Southern High Plains. For this study, the authors considered three varieties with three irrigation levels. The results show that newer indeterminate picker type varieties yielded higher, had greater response to irrigation, and produced larger profit margins than traditional determinate stripper type varieties.
Clear correspondence between theoretical and experimentallight propagation characteristics in photonic crystal waveguides (2001)

Single line defect optical waveguides in a photonic crystal airbridge fabricated into a silicon-on-insulator wafer with a minimum propagation loss of 11 dB/mm are demonstrated. The observed propagation loss spectrum and near field patterns at fibre communication wavelengths agree well with those expected from the photonic band calculation.
A novel compensation method of damping asymmetry based on piezoelectric electrodes for cylindrical resonators (2018)

The damping asymmetry of cylindrical resonators is one of the major sources which result in the gyroscope's drift. In this paper, a new approach for compensating the damping asymmetry of cylindrical resonators is proposed. The damping asymmetry compensation model is established to analyze the additional damping's influences. Furthermore, piezoelectric electrodes' effects on the cylindrical resonator's damping characteristic are figured out through the finite element simulation. At last, these theoretical analysis and simulation results are utilized to compensate the damping asymmetry of cylindrical resonators, which is also verified by experiments.
The advanced improvement of PN mesa junction diode prepared by silicon-wafer direct bonding (2002)

The key processes of Silicon-Wafer Direct Bonding (SDB), hydrophilic surface formation and optimum two-step heat treatment have been developed. However, there are still many unknown factors, such as native oxide, defects etc., influencing the characteristics of the bonded interface of Si/Si. The additional wafer treatment in diluted HF solution after hydrophilic surface formation can not only etch off native oxide but also form hydrophilic surface, and provide a voidless Si/Si bonding interface. The electrical characteristics of both N/sup +//P and N/P junction mesa diodes prepared by this novel SDB process were examined. Both of them have excellent electrical characteristics and their breakdown voltages are all over 280 V.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Design, fabrication, and characterization of antenna-coupled metal-oxide-metal diodes for dual-band detection (2005)

Two designs for antenna-coupled Ni-NiO-Ni diodes are fabricated and tested for dual-band detection in the millimeter-wave (MMW), 94-GHz, and infrared (IR), 28.3-THz, frequencies. The detector noise, antenna receiving properties, and noise equivalent power (NEP) are measured. The simultaneous dual-band response is verified.
Hop Aroma Component Profile and the Aroma Unit (1992)

More than 250 essential oil components have been identified in hops by capillary gas chromatography. The hop aroma component profile (HACP), quantitatively measured as nanoliters per gram of hops (1 ppm, v/w), is composed of 22 of these hop oil compounds that have been reported to affect hop aroma. Similar steam distillation methods were used to measure the HACP of hops, wort, and beer. HACP analysis of seven commercial hop pellet samples (Cascade, Chinook, Cluster, Hallertau, Saaz, Tettnang, and Willamette) were made. It was found that within one variety, the total HACP could vary by as much as 50%. Subsequently, the hop aroma unit, which consists of 1 nl/g (1 ppm, v/ w), the sum of the 22 hop oil constituents, was conceived. The hop aroma unit can be used as a standard measure of hop aroma content, just as the bitterness unit is used to measure bitterness.
Demonstration of a new oscillator based on real-space transfer in heterojunctions (1982)

A new real-space transfer oscillator is demonstrated in a layered GaAs/nAlGaAs heterojunction. A dc bias field, plus the ac oscillating field, is applied parallel to the layer interfaces to modulate the electron transfer from the GaAs layers to the nAlGaAs layers. This periodic electron transfer results in the ac current being 180° out of phase with the ac voltage and power being generated. A unique characteristic of this oscillator is that the electron transit times are associated with transverse dimensions and not dimensions between the ohmic contacts which should permit its extension to very high frequencies.
Phage display of functional αβ single-chain T-cell receptor molecules specific for CD1b:Ac2SGL complexes from Mycobacterium tuberculosis-infected cells (2013)

The development of molecules specific for M. tuberculosis-infected cells has important implications, as these tools may facilitate understanding of the mechanisms regulating host pathogen interactions in vivo. In addition, development of new tools capable to targeting M. tuberculosis-infected cells may have potential applications to diagnosis, treatment, and prevention of tuberculosis (TB). Due to the lack of CD1b polymorphism, M. tuberculosis lipid-CD1b complexes could be considered as universal tuberculosis infection markers. The aim of the present study was to display on the PIII surface protein of m13 phage, a human αβ single-chain T-cell receptor molecule specific for CD1b:2-stearoyl-3-hydroxyphthioceranoyl-2´-sulfate-α-α´-D-trehalose (Ac₂SGL) which is a complex presented by human cells infected with M. tuberculosis. The results showed the pIII fusion particle was successfully displayed on the phage surface. The study of the recognition of the recombinant phage in ELISA and immunohistochemistry showed the recognition of CD1b:Ac₂SGL complexes and cells in human lung tissue from a tuberculosis patient respectively, suggesting the specific recognition of the lipid-CD1b complex.
Ibuprofen and Glutathione Conjugate as a Potential Therapeutic Agent for Treating Alzheimer's Disease (2010)

Non-steroidal anti-inflammatory drugs (NSAIDs) and antioxidant therapy might protect against the development of Alzheimer's disease (AD). In the present work, we synthesized a molecular combination of glutathione (GSH) and ibuprofen (IBU) via an amide bond and investigated its potential for targeted delivery of the parent drugs to neurons, where cellular oxidative stress and inflammation are related to AD. Evaluation of its physicochemical and in-vitro antioxidant properties indicated that compound 1 exhibits good stability toward human plasma enzymatic activity, and, like GSH, displays in-vitro free radical scavenging activity in a time and concentration-dependent manner. The new compound was also assessed by infusion in a rat model for Alzheimer's disease for its potential to antagonize the deleterious structural and cognitive effects of β-amyloid(1-40). In behavioral tests of long-term spatial memory, animals treated with codrug 1 performed significantly better than those treated with β-amyloid (Aβ) peptide. Histochemical findings confirmed the behavioral data, revealing that Aβ protein was less expressed in cerebral cortex treated with 1 than that treated with IBU. Taken together, the present findings suggest that conjugate 1 treatment may protect against the oxidative stress generated by reactive oxygen species (ROS) and the cognitive dysfunction induced by intracerebroventricular (i.c.v.) infusion of Aβ(1-40) in rats, and thus that codrug 1 could prove useful as a tool for controlling AD induced cerebral amyloid deposits and behavioral deterioration.
Crosstalk noise reduction in synthesized digital logic circuits (2003)

As CMOS technology scales into the deep submicrometer regime, digital noise is becoming a metric of importance comparable to area, timing, and power, for analysis and design of CMOS VLSI systems. Noise has two detrimental effects in digital circuits: First, it can destroy logical information carried by a circuit net. Second, it causes delay uncertainty: Non critical paths might become critical because of noise. As a result, circuit speed becomes limited by noise, primarily because of capacitive coupling between wires. Most design approaches address the crosstalk noise problem at the layout generation stage, or via postlayout corrections. With continued scaling, too many circuit nets require corrections for noise, causing a design convergence problem. This work suggests to consider noise at the gate-level netlist generation stage. The paper presents a simplified analysis of on-chip crosstalk models, and demonstrates the significance of crosstalk between local wires within synthesized circuit blocks. A design flow is proposed for automatically synthesizing CMOS circuits that have improved robustness to noise effects, using standard tools, by limiting the range of gate strengths available in the cell library. The synthesized circuits incur a penalty in area/power, which can be partially recovered in a single postlayout corrective iteration. Results of design experiments indicate that delay uncertainty is the most important noise-related concern in synthesized static CMOS logic. Using a standard synthesis methodology, critical path delay differences up to 18% of the clock cycle time have been observed in functional blocks of microprocessor circuits. By using the proposed design flow, timing uncertainty was reduced to below 3%, with area and power penalties below 20%.
Palladium‐catalyzed synthesis of 8‐allyl or 8‐prenylcoumarins by using organotin reagents as multicoupling nucleophiles (2012)

In this study, 8‐iodocoumarin derivatives underwent a direct Stille cross‐coupling reaction with several organotin compounds in the presence of a catalytic amount of Pd(PPh 3 ) 4 in DMF at 80 °C. The cross‐coupling reaction proceeded smoothly under mild conditions and permitted the formation of 8‐allyl or 8‐prenylcoumarin compounds in good yields. Copyright © 2012 John Wiley & Sons, Ltd.
Soil Organic Matter, CEC, and Moisture Sensing with a Portable NIR Spectrophotometer (1993)

Soil reflectance data were collected with a portable near infrared (NIR) spectrophotometer and were correlated with soil organic matter content in laboratory and field tests. Laboratory calibrations yielded an r2 of 0.89 and a standard error of prediction of 0.40% organic matter with 30 representative Illinois soils at 1.5 MPa and 0.033 MPa moisture tension levels. Limited in-furrow field operation produced much higher errors, due to the movement of soil past the sensor during data acquisition. Estimation of cation exchange capacity (CEC) and soil moisture content was also accomplished in the laboratory.
Wavelength dependence of the modal refractive index in 1.3 μm InGaAsP, AlGaInAs and GaInNAs lasers using high pressure (2003)

Abstract We report the variation of the modal refractive index at threshold with lasing wavelength in 1.3 μm InGaAsP, AlGaInAs and GaInNAs multiple quantum well lasers by measuring mode spectra under pressure up to 15 kbar. It is shown that the decrease of index with lasing wavelength is about 4% on average in the pressure range studied. We also find that the modal refractive index reduces with increasing injection current and is very sensitive to the injection current around laser threshold.
[A research on rotaviral diarrhea outbreak in Guangxi Province]. (2008)

G1P[8] rotavirus is the etiologic agents of this diarrhea outbreak. In addition, adults were included in this outbreak.
Removal of blocking and ringing artifacts in transform coded images (2002)

Block-based discrete cosine transform (BDCT) image coding techniques are widely used in image and video compression applications such as JPEG and MPEG. At a moderate bit rate, the BDCT is usually a quite satisfactory solution to most of practical coding applications. However, for high compression rates it produces noticeable blocking and ringing artifacts in the decompressed image. We propose a novel post-processing algorithm to remove the blocking and ringing effects at low bit rates. The main steps in this algorithm include block classification, boundary low-pass filtering and mid-point interpolation, edge detection and filtering, and DCT coefficient constraint. The improvement is demonstrated both subjectively and objectively.
Simultaneous Dexamethasone Intravitreal Implant and Anti-VEGF Therapy for Neovascular Age-Related Macular Degeneration Resistant to Anti-VEGF Monotherapy (2017)

Combined treatment of anti-VEGF with the dexamethasone intravitreal implant is a viable alternative for treatment-resistant nvAMD, and may reduce treatment burden. Earlier treatment with dual therapy may be beneficial to maximize anatomic and visual outcomes in these patients.