gebhart/patrepeval huggingface dataset:
Infectivity and Pathogenicity of Nodamura Virus for Mosquitoes (1980)

SUMMARY Nodamura virus multiplied and caused paralysis and death when injected into the thoraces of adult Aedes albopictus and Toxorhynchites amboinensis mosquitoes but not when similarly injected into Culex quinquefasciatus adults. A. albopictus also became infected after ingesting a Nodamura virus suspension or after immersion in a virus suspension as larvae, but they did not die. Head squash preparations of the injected insects, examined by indirect fluorescent antibody technique, showed large amounts of Nodamura virus antigen in the brain regardless of the mode of infection. Nodamura virus was isolated from and titrated in mosquito cell (AP-61) cultures. Comparative titrations indicate that this method of assay is more sensitive than intracerebral inoculation of infant mice.
Influence of parameter estimation uncertainty in Kriging: Part 1 - Theoretical Development (2001)

Abstract. This paper deals with a theoretical approach to assessing the effects of parameter estimation uncertainty both on Kriging estimates and on their estimated error variance. Although a comprehensive treatment of parameter estimation uncertainty is covered by full Bayesian Kriging at the cost of extensive numerical integration, the proposed approach has a wide field of application, given its relative simplicity. The approach is based upon a truncated Taylor expansion approximation and, within the limits of the proposed approximation, the conventional Kriging estimates are shown to be biased for all variograms, the bias depending upon the second order derivatives with respect to the parameters times the variance-covariance matrix of the parameter estimates. A new Maximum Likelihood (ML) estimator for semi-variogram parameters in ordinary Kriging, based upon the assumption of a multi-normal distribution of the Kriging cross-validation errors, is introduced as a mean for the estimation of the parameter variance-covariance matrix. Keywords: Kriging, maximum likelihood, parameter estimation, uncertainty
pSITE Vectors for Stable Integration or Transient Expression of Autofluorescent Protein Fusions in Plants: Probing <i>Nicotiana benthamiana-</i>Virus Interactions (2007)

Plant functional proteomics research is increasingly dependent upon vectors that facilitate high-throughput gene cloning and expression of fusions to autofluorescent proteins. Here, we describe the pSITE family of plasmids, a new set of Agrobacterium binary vectors, suitable for the stable integration or transient expression of various autofluorescent protein fusions in plant cells. The pSITE vectors permit single-step Gateway-mediated recombination cloning for construction of binary vectors that can be used directly in transient expression studies or for the selection of transgenic plants on media containing kanamycin. These vectors can be used to express native proteins or fusions to monmeric red fluorescent protein or the enhanced green fluorescent protein and its cyan and yellow-shifted spectral variants. We have validated the vectors for use in transient expression assays and for the generation of transgenic plants. Additionally, we have generated markers for fluorescent highlighting of actin filaments, chromatin, endoplasmic reticulum, and nucleoli. Finally, we show that pSITE vectors can be used for targeted gene expression in virus-infected cells, which should facilitate high-throughput characterization of protein dynamics in host-virus interactions.
A digital filter structure requiring only m-bit delays, shifters, inverters, and m-bit adders plus simple logic circuitry (1980)

digital filter structure is presented which requires only <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</tex> -bit adders, shifters, and inverters, where <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</tex> is the state-variable word length, to exactly realize the arithmetic operations for state equations. It is assumed that the coefficients in the state equations are finite-wordlength binary numbers; the canonical signed digit code is used to simplify these coefficients. All bits (including underflow bits) of the next state variables are computed, using two's complement (2's C) arithmetic, so the only source of roundoff error is the truncation of the exact next-state variables to <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</tex> bits. Simple logic circuitry produces magnitude truncation, which suppresses all zero-input limit cycles if a diagonal Lyapunov function exists. Thus the above structure is applied to state equations derived for wave digital filters, which possess a diagonal Lyapunov function and yield short coefficient word lengths due to low sensitivity properties. A computer-simulated example, a third-order elliptic low-pass filter, is given with unit-sample response and frequency response for <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">m</tex> equal to 8 and 16 bits.
Inhibitory Effect of <i>p</i>-Hydroxybenzyl Alcohol on Tyrosinase Activity and Melanogenesis (2007)

Tyrosinase is a key enzyme catalyzing the rate-limiting step for the biosynthesis pathway of melanin pigment, which is the most important determinant of the color of skin. Inhibiting tyrosinase and repressing melanocyte metabolism can reduce melanin production. Among the possible melanin reducing compounds, tyrosinase inhibitors are most promising for treating pigmentation and are used as skin-whitening agents in the cosmetic industry. In our investigation, some new tyrosinase inhibitors from plants have been identified to have high tyrosinase inhibitory activity. Specifically, p-hydroxybenzyl alcohol (4HBA) was found to inhibit the monophenolase activity of mushroom tyrosinase. When 4HBA binds with the enzyme, conformation of the enzyme is altered and its activity decreases. The inhibitory effect of 4HBA on melanogenesis has been studied using cultured mouse melanoma cells. Melanin synthesis in cell culture with 4HBA at 1.0 mM was decreased to 45% of control and below 1.0 mM there was no effect on cell growth. The inhibitory effects of 4HBA on melanogenesis are due to the direct inhibition of melanosomal tyrosinase activity, rather than to the suppression of tyrosinase gene. These results showed that 4HBA is a promising and safe agent for skin whitening.
Protein folds and their recognition from sequence (1996)

Abstract Until relatively recently there had been little progress in methods for protein structure prediction (1). Secondary structure prediction had hardly changed since the early 1970s with little improvement in accuracy despite much work and an increase in the size of the database used to derive the parameters (2). Methods for tertiary structure prediction were hampered by the relatively low accuracy of secondary structure prediction. The latter (3–7) were predominantly knowledge-based, in that most methods relied on secondary structure propensities derived from proteins of known structure. As the number of structures rapidly increased during the 1980s, it became apparent that there are also rules which govern the arrangement of secondary structures into their globular fold. For example the connections in ²±² units are always right-handed (8,9), and helices and sheets tend to pack in preferred orientations (10–12). The challenge at the end of the 1980s was how to use this information to improve tertiary structure prediction.
IL-13: A Promising Therapeutic Target for Bronchial Asthma (2006)

The incidence of allergic diseases has dramatically increased in recent decades, especially in urban and industrialized areas. It is important socially as well as medically to establish more useful strategies to overcome allergic disorders. Bronchial asthma is a complex disease characterized by airway inflammation involving a Th2-cytokine, interleukin (IL)-13. A substantial body of evidence has accumulated pointing to the pivotal role of IL-13 in the pathogenesis of bronchial asthma, based on mainly analyses of mouse models. In addition to such analyses, the high expression of IL-13 in lesions and genetic association of several genes coding IL-13 signaling molecules with bronchial asthma have raised the possibility that IL-13 plays a pivotal role in the onset or exacerbation of human bronchial asthma. Therefore, IL-13 and its signal pathway are thought to be promising targets to develop a therapeutic agent for bronchial asthma. In this article, we describe how IL-13 is involved in the pathogenesis of bronchial asthma and then how therapeutic agents to block IL-13 signals are developed for bronchial asthma.
Inhibition of Poly-(ADP-Ribose) Polymerase Protects the Kidney in a Canine Model of Endotoxic Shock (2015)

Poly-(ADP-ribose) polymerases (PARPs), a super family of enzymes, play important roles in preserving genomic integrity, regulating transcriptions, protecting telomeres and determining cell fate. PARP overactivation leads to metabolic disorder and cell injury via depletion of energy substance. However, it is still unclear whether PARP overactivation happens during acute kidney injury (AKI) caused by endotoxic shock (ES). Here, we built a canine model of lipopolysaccharide-induced ES to explore the role of PARP during the development AKI. We also used an intravenous injection of 3-aminobenzamide (3-AB) to further explore whether PARP inhibition rescues the kidney from injury. Cell fate and energy metabolism were detected to explore the underlying mechanisms. As a result, Western blot and immunohistochemistry assays showed PARP overactivation in the very early phase of ES. Through PARP inhibition by 3-AB, we observed significant improvement of systemic hemodynamics, renal hemodynamics, renal oxygen metabolism and renal tubular cell apoptosis. These findings indicated that overactivation of PARP plays an important role in septic AKI. Inhibition of PARP overactivation may protect renal function against hemodynamic disorder, renal metabolism disturbance and renal cell apoptosis during endotoxic AKI.
A software reuse system based on natural language specifications (2002)

Promoting software reuse practice requires more effective support. We discuss some problems in current software reuse systems and how current research in retrieval through natural language specifications addresses them. We introduce work in progress for a software reuse system that aims to provide high precision in retrieval by processing both queries in natural language and descriptions of components in a software library. Additional support for application developers (for understanding and adapting software components) and for library managers (for creation, organization and reorganization of reusable components) is also discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Telechelic sulfate ionomers. I. Preparation and solution and thermal properties (1988)

Abstract New telechelic ionomers with zinc and sodium sulfate salt terminal groups on hydrogenated polybutadiene (HPB) backbones ( I ) were prepared from hydroxyl‐terminated hydrogenated polybutadiene (HTHPB) of three different molecular weights (1350, 2100, and 3200 g/mol). Quantitative acid‐base titration, elemental analysis, and NMR spectroscopy were used to verify the structure, and further characterization included differential scanning calorimetry (DSC) and solution viscometry. The DSC results indicated that the ionomers are free of impurities within the limit of the resolution of the method. Glass transition temperatures determined by DSC indicated that the elevation in glass transition temperature by ionic crosslinking was most strongly dependent on the molecular weight of the backbone of the telechelic ionomer. The solution viscometry results showed that the sulfation reaction did not cause either covalent crosslinking or chain scission. Furthermore, the solubility characteristics of the sulfate‐terminated hydrogenated polybutadiene (STHPB) oligomers were shifted towards a preference for polar solvents by the presence of salt groups. The lower molecular weight ionomers of the series showed polyelectrolyte‐like extension at very dilute concentrations in polar solvents. The onset of polymer gelation in hexane was observed for the ionomers which had the highest molecular weight backbones.
Resveratrol derivatives potently induce apoptosis in human promyelocytic leukemia cells (2003)

Resveratrol has been shown to possess antioxidant and anticancer activities, but little is known on the effect of resveratrol derivatives. Recently we have isolated resveratrol and its dimers and trimers from peony (Paeonia lactiflora) seeds, and reported their strong antioxidant and cytotoxic activity. In the present study, we have evaluated cellular effects of resveratrol derivatives; viniferin, gnetin H, and suffruticosol B on the proliferation and apoptosis in HL-60 cells in vitro. All resveratrol and its derivatives reduced viability of HL-60 cells in a dose-dependent manner with their IC50 values of 20-90 µM. Ascending orders of IC50 values were suffruticosol B, gnetin H, viniferin and resveratrol respectively. HL-60 cells treated with the four stilbenes exhibited the distinct morphological changes characteristics of cell apoptosis such as chromatin condensation, apoptotic bodies, and DNA fragmentations. A time-dependent histogram of the cellular DNA analyzed by flow cytometry revealed a rapid increase in subdiploid cells and a concomitant decrease in diploid cells exposed to 100 µM resveratrol for 0-24 h. Cells treated with 25 µM of resveratrol, viniferin, gnetin H, and suffruticosol B for 24 h resulted in increment of sub-G1 population by 51, 5, 11 and 59%, respectively. Treatment of cells with 0-20 µM resveratrol for 5 h produced a concentration-dependent decrease in cytochrome P450 (CYP) 1B1 mRNA levels. Suffruticosol B also suppressed CYP1B1 gene expression. These results demonstrated that resveratrol oligomers also strongly suppressed HL-60 cell proliferation, and induced DNA damage. In addition, CYP1B1 gene supression may suggest an involvement in the resveratrol-induced apoptosis in HL-60 cells.
Angle-independent structural coloured amorphous arrays (2012)

We are able to observe a colour due to the interference of light from microstructures composed of different refractive index materials that is comparable to the visible wavelength of light; such a colour is called a structural colour. Because structural colour is fadeless and no energy is lost from the colour mechanism, structurally coloured materials are expected to be used for energy-saving reflective displays and sensors. Previously, however, the word “iridescence” rather than “structural colour” was used to describe the property of a surface that appears to change colour as the viewing angle or the angle of light illumination changes. Thus, people who are aware of the concept of interference colour have a strong impression that all structurally coloured materials change hue when viewed from different angles, as indicated by the term “iridescence.” In fact, most artificial structurally coloured materials that we and other groups have studied so far change their hue depending on the viewing and light illumination angles because these structural colours are derived from Bragg reflection. Such angle dependence presents a barrier for developing displays and sensors using structurally coloured materials. Therefore, my group has been working to develop angle-independent structural coloured materials. The latest most notable ones are amorphous array systems. In this review, I first introduce the microstructures and optical properties of low-angle-dependent structurally coloured amorphous arrays in biological systems, then describe the fabrication and the optical nature of the artificially prepared imitations of such biological systems, and finally, present the related theoretical studies.
Field-Driven Mott Gap Collapse and Resistive Switch in Correlated Insulators (2016)

Mott insulators are "unsuccessful metals" in which Coulomb repulsion prevents charge conduction despite a metal-like concentration of conduction electrons. The possibility to unlock the frozen carriers with an electric field offers tantalizing prospects of realizing new Mott-based microelectronic devices. Here we unveil how such unlocking happens in a simple model that shows the coexistence of a stable Mott insulator and a metastable metal. Considering a slab subject to a linear potential drop, we find, by means of the dynamical mean-field theory, that the electric breakdown of the Mott insulator occurs via a first-order insulator-to-metal transition characterized by an abrupt gap collapse in sharp contrast to the standard Zener breakdown. The switch on of conduction is due to the field-driven stabilization of the metastable metallic phase. Outside the region of insulator-metal coexistence, the electric breakdown occurs through a more conventional quantum tunneling across the Hubbard bands tilted by the field. Our findings rationalize recent experimental observations and may offer a guideline for future technological research.
Lower Order Modulation Aided BER Reduction in OFDM With Index Modulation (2018)

Orthogonal frequency division multiplexing with index modulation (OFDM-IM) is a multi-carrier transmission scheme in which a set of sub-carriers is chosen and modulated according to the incoming bit-stream. It is already established that for the same average bits per channel use, OFDM-IM can provide a better error rate performance when compared to conventional OFDM. In this letter, we propose a novel method to further improve the error rate performance of OFDM-IM with the aid of lower order signal constellations, by using fewer bits for symbol selection and using the remaining bits to select the index combinations. As the increased number of indexing bits demand more index combinations for mapping them, certain index combinations are reused, with the reuse made unambiguous by rotating lower order constellation. This approach provides significant signal-to-noise ratio gains without compromising spectral efficiency when compared to existing OFDM-IM schemes.
Radiotherapy and immune checkpoint blockades: a snapshot in 2016 (2016)

Immune checkpoint blockades including monoclonal antibodies (mAbs) of cytotoxic T-lymphocyte antigen-4 (CTLA-4), programmed death-1 (PD-1), and programmed death-ligand 1 (PD-L1) have been emerged as a promising anticancer therapy. Several immune checkpoint blockades have been approved by US Food and Drug Administration (FDA), and have shown notable success in clinical trials for patients with advanced melanoma and non-small cell lung cancer. Radiotherapy is a promising combination partner of immune checkpoint blockades due to its potent pro-immune effect. This review will cover the current issue and the future perspectives for combined with radiotherapy and immune checkpoint blockades based upon the available preclinical and clinical data.
Flow properties of foam with and without solid particles (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFlow properties of foam with and without solid particlesNandu N. Thondavadi and Robert LemlichCite this: Ind. Eng. Chem. Process Des. Dev. 1985, 24, 3, 748–753Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/i200030a038https://doi.org/10.1021/i200030a038research-articleACS PublicationsRequest reuse permissionsArticle Views277Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Plasmonic Optical Tweezers toward Molecular Manipulation: Tailoring Plasmonic Nanostructure, Light Source, and Resonant Trapping (2014)

This Perspective describes recent progress in optical trappings of nanoparticles based on localized surface plasmon. This plasmonic optical trapping has great advantages over the conventional optical tweezers, being potentially applicable for a molecular manipulation technique. We review this novel trapping technique from the viewpoints of (i) plasmonic nanostructure, (ii) the light source for plasmon excitation, and (iii) the polarizability of the trapping target. These findings give us future outlook for plasmonic optical trapping. In addition to a brief review, recent developments on plasmonic optical trapping of soft nanomaterials such as proteins, polymer chains, and DNA will be discussed to point out the important issue for further development on this trapping method. Finally, we explore new directions of plasmonic optical trapping.
Fragment‐Based Drug Discovery. (2004)

Abstract For Abstract see ChemInform Abstract in Full Text.
Undesired light images associated with ovoid intraocular lenses (1993)

Ovoid intraocular lenses (IOLs) accounted for approximately 35% of the lenses implanted in 1991. Despite their popularity among surgeons, patient observations and complaints suggest that ovoid lenses are associated with undesired optical images postoperatively. A pilot clinical study was carried out to test that hypothesis. A multicentered investigation of 289 cases with well-centered IOLs determined that 45% of 168 patients with ovoid lenses observed unwanted optical phenomena at some time after surgery, whereas 17% of 121 patients with round IOLs had similar complaints. Based upon the preliminary clinical study a laboratory investigation was designed to assess optical performance differences between ovoid and round IOLs. One testing system used scatterometry and a second, ray tracing analysis. Both methods determined that the truncated, thickened edge of ovoid lenses was associated with significant light scattering, possibly accounting for the extraneous light images observed by some patients. The results of the clinical and laboratory investigations suggest that undesired optical images produced by ovoid IOLs are due to lens shape rather than to the reduced dimension of the optic.
Sintering of PZT Ceramics: I, Atmosphere Control (1983)

Methods of PbO atmosphere control during the sintering of PZT ceramics are briefly reviewed. The problem of atmosphere control of PZT compositions containing a PbO excess (PbO/(Zr,Ti)O 2 >1) is discussed. Weight‐loss experiments were performed to compare with behavior predicted by reference to the PbO‐rich part of the ternary PbO‐ZrO 2 ‐TiO 2 phase diagram. The results indicated that the PbO activity of a PZT composition is a function of the excess PbO content, contrary to earlier reports. Experimental procedures were established in order to control the PbO content within satisfactory limits. The effects of various packing powders on the PbO content of sintered PZT compacts were evaluated.