gebhart/patrepeval huggingface dataset:
Frataxin expression rescues mitochondrial dysfunctions in FRDA cells (2001)

Friedreich's ataxia (FRDA) is the result of mutations in the nuclear-encoded frataxin gene, which is expressed in mitochondria. Several lines of evidence have suggested that frataxin is involved in mitochondrial iron homeostasis. We have transfected the frataxin gene into lymphoblasts of FRDA compound heterozygotes (FRDA-CH) with deficient frataxin expression to produce FRDA-CH-t cells in which message and protein are rescued to near-physiological levels. FRDA-CH cells were more sensitive to oxidative stress by challenge with free iron, hydrogen peroxide and the combination, consistent with a Fenton chemical mechanism of pathophysiology, and this sensitivity was rescued to control levels in FRDA-CH-t cells. Iron challenge caused increased mitochondrial iron levels in FRDA-CH cells, and a decreased mitochondrial membrane potential (MMP), both of which were rescued in FRDA-CH-t cells. The rescue of the low MMP, and high mitochondrial iron concentration by frataxin overexpression suggests that these cellular phenotypes are relevant to the central pathophysiological process in FRDA which is aggravated by exposure to free iron. However, even at physiological iron concentrations, FRDA-CH cells had decreased MMP as well as lower activities of aconitase and ICDH (two enzymes supporting MMP), and twice the level of filtrable mitochondrial iron (but no increase in total mitochondrial iron), and the observed phenotypes were either fully or partially rescued in FRDA-CH-t cells. Free iron is known to be toxic. The observation that frataxin deficiency (either directly or indirectly) causes an increase in filtrable mitochondrial iron provides a new hypothesis for the mechanism of cell death in this disease, and could be a target for therapy.
Transdifferentiation: why and how? (2011)

Cell therapy is based on the replacement of damaged cells in order to restore injured tissues. The first consideration is that an abundant source of cells is needed; second, these cells should be immunologically compatible with the guest and third, there should be no real threat of these cells undergoing malignant transformation in the future. Given these requirements, already differentiated adult cells or adult stem cells obtained from the body of the patient appear to be the ideal candidates to meet all of these demands. The utilization of somatic cells also avoids numerous ethical and political drawbacks and concerns. Transdifferentiation is the phenomenon by which an adult differentiated cell switches to another differentiated cell. This paper reviews the importance of transdifferentiation, discussing the cells that are suitable for this process and the methods currently employed to induce the change in cell type.
Adsorption of comb copolymers on weakly attractive solid surfaces (2005)

In this work continuum and lattice Monte Carlo simulation methods are used to study the adsorption of linear and comb polymers on flat surfaces. Selected polymer segments, located at the tips of the side chains in comb polymers or equally spaced along the linear polymers, are attracted to each other and to the surface via square-well potentials. The rest of the polymer segments are modeled as tangent hard spheres in the continuum model and as self-avoiding random walks in the lattice model. Results are presented in terms of segment-density profiles, distribution functions, and radii of gyration of the adsorbed polymers. At infinite dilution the presence of short side chains promotes the adsorption of polymers favoring both a decrease in the depletion-layer thickness and a spreading of the polymer molecule on the surface. The presence of long side chains favors the adsorption of polymers on the surface, but does not permit the spreading of the polymers. At finite concentration linear polymers and comb polymers with long side chains readily adsorb on the solid surface, while comb polymers with short side chains are unlikely to adsorb. The simple models of comb copolymers with short side chains used here show properties similar to those of associating polymers and of globular proteins in aqueous solutions, and can be used as a first approximation to investigate the mechanism of adsorption of proteins onto hydrophobic surfaces.
The Circles of Latitude: Adoption and Usage of Location Tracking in Online Social Networking (2009)

This paper reports preliminary results of an ongoing ethnographic study of peoplepsilas attitudes towards and adoption of Google Latitude, a location-tracking technology for mobile devices. In order to understand barriers to adoption, participants include both users and non-users of Latitude, and those whose usage has dropped off. The report focuses on how participants perceive Latitude to be conceptually situated within the ecology of social networking and communication technologies. Earlier work on user attitudes with regard to location tracking emphasized potential privacy concerns. In our research we also identified privacy concerns, but additionally several other more salient tensions such as adoption trends, social conformance, audience management, and information filtering.
Dilute Self-Healing Hydrogels of Silk-Collagen-Like Block Copolypeptides at Neutral pH (2014)

We report on self-healing, pH-responsive hydrogels that are entirely protein-based. The protein is a denovo designed recombinant triblock polypeptide of 66 kg/mol consisting of a silk-like middle block (GAGAGAGH)48, flanked by two long collagen-inspired hydrophilic random coil side blocks. The pH-dependent charge on the histidines in the silk block controls folding and stacking of the silk block. At low pH the protein exists as monomers, but above pH 6 it readily self-assembles into long fibers. At higher concentrations the fibers form self-healing physical gels. Optimal gel strength and self-healing are found at a pH of around 7. The modulus of a 2 wt % gel at pH 7 is G' = 1700 Pa. Being protein-based, and amenable to further sequence engineering, we expect that these proteins are promising scaffold materials to be developed for a broad range of biomedical applications.
Dynamic test compression using statistical coding (2002)

Test compression/decompression is an efficient method for reducing the test application cost. In this paper we propose a test generation method for obtaining test-patterns suitable to test compression by statistical coding. In general, an ATPG generates a test-pattern that includes don't-care values. In our method, such don't-care values are specified based on an estimation of the final probability of 0/1 occurrence in the resultant test set. Experimental results show that our method can generate test patterns that are able to be highly compressed by statistical coding, in small computational time.
Segmentation of three-dimensional images using non-rigid registration: methods and validation with application to confocal microscopy images of bee brains (2003)

This paper describes the application and validation of automatic segmentation of three-dimensional images by non-rigid registration to atlas images. The registration-based segmentation technique is applied to confocal microscopy images acquired from the brains of 20 bees. Each microscopy image is registered to an already segmented reference atlas image using an intensity-based non-rigid image registration algorithm. This paper evaluates and compares four different approaches: registration to an individual atlas image (IND), registration to an average shape atlas image (AVG), registration to the most similar image from a database of individual atlas images (SIM), and registration to all images from a database of individual atlas images with subsequent fuzzy segmentation (FUZ). For each strategy, the segmentation performance of the algorithm was quantified using both a global segmentation correctness measure and the similarity index. Manual segmentation of all microscopy images served as a gold standard. The best segmentation result (median correctness 91 percent of all voxels) was achieved using the FUZ paradigm. Robustness was also the best for this strategy (minimum correctness over all individuals 84 percent). The mean similarity index value of segmentations produced by the FUZ paradigm is 0.86 (IND, 0.81; AVG, 0.84; SIM, 0.82). The superiority of the FUZ paradigm is statistically significant (two-sided paired t-test, P<0.001).
Discovering human interactions in videos with limited data labeling (2015)

We present a novel approach for discovering human interactions in videos. Activity understanding techniques usually require a large number of labeled examples, which are not available in many practical cases. Here, we focus on recovering semantically meaningful clusters of human-human and human-object interaction in an unsupervised fashion. A new iterative solution is introduced based on Maximum Margin Clustering (MMC), which also accepts user feedback to refine clusters. This is achieved by formulating the whole process as a unified constrained latent max-margin clustering problem. Extensive experiments have been carried out over three challenging datasets, Collective Activity, VIRAT, and UT-interaction. Empirical results demonstrate that the proposed algorithm can efficiently discover perfect semantic clusters of human interactions with only a small amount of labeling effort.
Incorporation of a zinc phosphide rodenticide into integrated management of rats in sugarcane crops (2002)

Lethal control of large rodent populations using RATTOFF  zinc phosphide bait sachets now forms part of an integrated pest management (IPM) strategy that includes early detection of increasing rat populations, management of harbourage areas, and incrop weed control. This combined approach reduces the potential for large-scale rat plagues and reduces crop damage throughout the production cycle. It is important to maintain low rodent base populations within and around the crop because Rattus sordidus has one of the highest reproductive potentials of any native Australian Rattus species. The growth and maturation of weeds, grasses and seeds after rainfall provides rats with a source of protein required for reproduction. Therefore, weed control within and around crops also reduces nutritional support for breeding. If monitoring indicates that a base population exists early in the season, application of RATTOFF  from November to January will provide an optimum strategic window to further reduce base populations prior to the natural breeding season in autumn. In 2000–2001, an industrywide program was implemented to apply these principles to cane rat infestations at district and regional levels in order to mitigate overall damage potential. Over 2700 Queensland canegrowers participated in training on IPM for cane rats. An industry-wide monitoring system and database was re-established to assist in more effective management of this pest.
THE ISOLATION OF MOUSE HEPATOCYTE GAP JUNCTIONS (1972)

A method is reported for isolating a preparation of hepatic gap junctions from the mouse. The method involves a collagenase digestion, treatment with the detergent Sarkosyl NL-97, and ultrasonication, followed by sucrose gradient ultracentrifugation. A run with 36 animals yields 0.1-0.5 mg protein. Electron microscopy with thin-sectioning and negative staining techniques reveals that the final pellet is a very pure preparation of gap junctions, accompanied by a small amount of amorphous contamination. Polyacrylamide-gel electrophoresis of sodium dodecyl sulfate (SDS)-solubilized material shows one major protein in the junction, with an apparent mol wt of 20,000, and two minor components. Thin-layer chromatography demonstrates one major and one minor phospholipid, and some neutral lipid. Low-angle X-ray diffraction of wet and dried specimens show reflections which index on an 86 A center-to-center hexagonal lattice, corresponding closely to electron microscope data. Dried specimens also show a lamellar diffraction, corresponding to the total profile thickness of the junction (150 A).
Coherent beam combination of three ytterbium fiber amplifiers (2006)

Coherent beam combination of fiber laser array is an important technology in realizing high power fiber laser system. In this paper, master oscillator-power amplifier scheme is used to realize phase control of three ytterbium fiber amplifiers. In the experiments, the output power of each ytterbium fiber amplifier is not less than 1 W and the total output power of the three amplifiers is not less than 3 W. Phases are controlled by LiNbO3 phase modulator. The heterodyne detection control system works at 40 MHz and the control precision is l/20. The fibers are polarizedly maintained. The experimental results of both two and three fiber amplifiers are given. When the control loop is off, the far field interference pattern is dynamic and blurred. While the control loop is on, the far field
Sales: Transfer of Possession: Intention of Parties (1942)

Manlove brought an action against Maggart to recover possession of furniture which Manlove asserted belonged to him. He based this claim of ownership upon a written instrument entitled "Conditional sale note" whereby Maggart promised to pay Manlove $150. In the body of this instrument was the statement that "The express condition of the sale and purchase of one eleven piece mahogany dining room suite . . . for which this note is given, is such that the title and ownership of the above described property does not pass from the said Omer S. Manlove . . . until this note with interest is paid in full. Omer S. Manlove has full power to declare this note due, and take possession of said property at any time he may deem this note insecure." No other reference to the furniture, nor to its ownership, nor to an intention to transfer title, appears in the instrument. Manlove had never been in possession of the furniture; it had been bought from the administrator of an estate eleven months prior to the making of the so-called conditional sale note and had at that time been delivered by the administrator directly to Maggart. Prior to the. time of this sale by the administrator, Manlove, knowing that Maggart was interested in the furniture, had offered to lend him money with which to buy it. Payment to the administrator was made by Manlove with his personal check. The court held the jury justified in finding that title had passed directly from the administrator to Maggart; that Manlove had merely loaned him the money with which to pay for it; and that Manlove had no title upon which to sustain a replevin action. Manlove v. Maggart, (Ind. App. 1942) 41 N. E. (2d) 633.
The SADIC, a Precision Analog-Digital Converter (1953)

An accurate and reliable method for the conversion of analog voltages to equivalent digital values is described in which the basic operating mechanism is a self-balancing potentiometer having one thousand discrete balance positions. Applications to mechanized digital data handling systems are discussed.
Dendritic Crystallization of Ice from Aqueous Solutions (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDendritic Crystallization of Ice from Aqueous SolutionsP. K. Rohatgi, S. M. Jain, and C. M. Adams Jr.Cite this: Ind. Eng. Chem. Fundamen. 1968, 7, 1, 72–79Publication Date (Print):February 1, 1968Publication History Published online1 May 2002Published inissue 1 February 1968https://pubs.acs.org/doi/10.1021/i160025a013https://doi.org/10.1021/i160025a013research-articleACS PublicationsRequest reuse permissionsArticle Views112Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Data locality and load balancing in COOL (1993)

Large-scale shared memory multiprocessors typically support a multilevel memory hierarchy consisting of per-processor caches, a local portion of shared memory, and remote shared memory. On such machines, the performance of parallel programs is often limited by the high latency of remote memory references. In this paper we explore how knowledge of the underlying memory hierarchy can be used to schedule computation and distribute data structures, and thereby improve data locality. Our study is done in the context of COOL, a concurrent object-oriented language developed at Stanford. We develop abstractions for the programmer to supply optional information about the data reference patterns of the program. This information is used by the runtime system to distribute tasks and objects so that the tasks execute close (in the memory hierarchy) to the objects they reference. We demonstrate the effectiveness of these techniques by applying them to several applications chosen from the SPLASH parallel benchmark suite. Our experience suggests that improving data locality can be simple through a combination of programmer abstractions and smart runtime scheduling.
The Routine Measurement of Platelet Size Using Sodium Citrate Alone as the Anticoagulant (1993)

Mean platelet volume (MPV), a measure of platelet size, is becoming recognised as an important marker of platelet function. However, platelets swell in edetic acid (EDTA), the standard haematology anticoagulant, in a time-dependent manner making such measurements potentially unreliable. The effect of incubation time on MPV, and platelet distribution width (PDW), as measured in EDTA, low (1:9 volume/volume with blood) or high (1:4 v/v with blood) concentration sodium citrate was studied. MPV measured in high concentration sodium citrate did not change with time in contrast to MPV measured in either low concentration sodium citrate or EDTA which both increased in an inverse exponential fashion. MPV and PDW, measured in high concentration sodium citrate, had similar within-assay and between-assay coefficients of variation as other platelet, red cell and white cell haematology variables measured in EDTA: MPV 1.4%, 2.1%; PDW 1.4%, 1.5%; MCV 0.4%, 0.7%; PC 3.1%, 6.1%; WCC 1.5%, 7.3%; Hb 2.1%, 2.4% respectively. MPV measured in EDTA and corrected for incubation time approximated to, but was higher than, the MPV measured in high concentration sodium citrate. PDW correlated inversely with platelet count (r = -0.415, 2p < 0.001). MPV may be measured in sodium citrate (at 1:4 v/v with blood) alone with a better accuracy and reproducibility than similar measurements made in EDTA. Furthermore, such measurements are not influenced by incubation time, unlike for EDTA.
Knowledge-based graph document modeling (2014)

We propose a graph-based semantic model for representing document content. Our method relies on the use of a semantic network, namely the DBpedia knowledge base, for acquiring fine-grained information about entities and their semantic relations, thus resulting in a knowledge-rich document model. We demonstrate the benefits of these semantic representations in two tasks: entity ranking and computing document semantic similarity. To this end, we couple DBpedia's structure with an information-theoretic measure of concept association, based on its explicit semantic relations, and compute semantic similarity using a Graph Edit Distance based measure, which finds the optimal matching between the documents' entities using the Hungarian method. Experimental results show that our general model outperforms baselines built on top of traditional methods, and achieves a performance close to that of highly specialized methods that have been tuned to these specific tasks.
Emerging biological therapies in primary Sjogren's syndrome (2007)

Sjögren's syndrome (SS) is a systemic autoimmune disease that mainly affects the exocrine glands and usually presents as persistent dryness of the mouth and eyes. SS primarily affects white perimenopausal women, with an incidence of 4-5 cases per 100 000. Recent studies have analysed new therapeutic approaches, focusing mainly on the use of biological agents. B-cell targeted therapies seem to be the most promising agents in primary SS, especially rituximab, which has been used in more than 50 reported cases. Other promising B-cell targeted therapies include epratuzumab and belimumab, while T-cell targeted agents (efalizumab, abatacept, alefacept) should currently be considered as possible future options. In the near future, biological agents will play key roles in the treatment of severe involvement, broadening the therapeutic options in primary SS and offering a more optimistic point of view of the treatment of this disease, which, at present, is often considered to lack adequate specific therapy. However, the possible risks and benefits of using these agents should be carefully balanced, and a reasonable assessment of the risk of serious adverse events versus the benefits of treatment should be made. The use of biological agents targeting molecules and receptors involved in the aetiopathogenesis of primary SS opens a new era in the therapeutic management of patients with primary SS.
Toxic Protein Expression in <i>Escherichia Coli</i> Using a Rhamnose-Based Tightly Regulated and Tunable Promoter System (2006)

The refinement of tightly regulated prokaryotic expression systems that permit functional expression of toxic recombinant proteins is a continually evolving process. Unfortunately, the current best promoter options are either tightly repressed and produce little protein, or produce substantial protein but lack the necessary repression to avoid mutations stimulated by leaky expression in the absence of inducer. In this report, we present three novel prokaryotic expression constructs that are tightly regulated by L-rhamnose and D-glucose. These expression vectors utilize the Escherichia coli rhaT promoter and corresponding regulatory genes to provide titratable, high-level protein yield without compromising clone integrity. Together, these components may enable the stable cloning and functional expression of otherwise toxic proteins.