gebhart/patrepeval huggingface dataset:
Neural correlates of the DMT experience assessed with multivariate EEG (2019)

Studying transitions in and out of the altered state of consciousness caused by intravenous (IV) N,N-Dimethyltryptamine (DMT - a fast-acting tryptamine psychedelic) offers a safe and powerful means of advancing knowledge on the neurobiology of conscious states. Here we sought to investigate the effects of IV DMT on the power spectrum and signal diversity of human brain activity (6 female, 7 male) recorded via multivariate EEG, and plot relationships between subjective experience, brain activity and drug plasma concentrations across time. Compared with placebo, DMT markedly reduced oscillatory power in the alpha and beta bands and robustly increased spontaneous signal diversity. Time-referenced and neurophenomenological analyses revealed close relationships between changes in various aspects of subjective experience and changes in brain activity. Importantly, the emergence of oscillatory activity within the delta and theta frequency bands was found to correlate with the peak of the experience - particularly its eyes-closed visual component. These findings highlight marked changes in oscillatory activity and signal diversity with DMT that parallel broad and specific components of the subjective experience, thus advancing our understanding of the neurobiological underpinnings of immersive states of consciousness.
Photochemical attachment of biomolecules onto ﬁbre‐optics for construction of a chemiluminescent immunosensor (2004)

We report herein a simple and effective way to photochemically immobilize biomolecules onto a fibre-optic silica surface. The system is based on a photoreactive benzophenone derivative that is bound to SiO2 surfaces of the optical fibre via a silane anchor. The benzophenone derivative was 4-allyloxybenzophenone, synthesized by standard procedures that were later used to synthesize the 4-(3'-chlorodimethylsilyl) propyloxybenzophenone and 4-(3'-dichloromethylsilyl) propyloxybenzophenone by regular hydrosilation procedures. After silanization with the benzophenone derivatives, the fibres were immersed in a cholera toxin B subunit solution and illuminated with UV light (wavelength > 345 nm). As a result of the photochemical reaction, a thin layer of the antigen was covalently bound to the benzophenone-modified surface. The photochemically modified fibre-optics were then tested as immunosensors in the detection of cholera anti-toxin antibody and revealed through chemiluminescence measurements. A secondary antibody labelled with horseradish peroxidase acted as the marker for the cholera toxin antibody. A photo-electronic set-up was designed specifically to monitor the signal. The immunosensor system was shown to be both specific and sensitive. The lowest rabbit serum titre detected was 1:1 700,000.
Posterior composite restoration with stamp technique (2020)

Occlusal discrepancy after direct restorations often leads to discomfort to the patients. Newer methods are developed to overcome the previous problems and to reduce the time required. A stamp technique is proposed which is an easy procedure to recreate accurate occlusal topography for a direct composite resin restoration effectively and efficiently. This technique is indicated when the preoperative anatomy of the tooth is intact and not destructed or lost due to the carious lesion.
Studies on Egg Drop Syndrome (EDS) and Chick Embryo Lethal Orphan (CELO) Avian Adenovirus DNAs by Restriction Endonucleases (1981)

Restriction endonucleases EcoRI, BamHI, HindIII, BglI, BglII, HpaI and PstI recognized 48 cleavage sites in EDS adenovirus strain B8/78 DNA, whereas CELO virus DNA was cut into 61 fragments by the same enzymes. No similarity could be detected in the restriction patterns obtained from the two avian adenoviruses. The calculated mol. wt. of B8/78 DNA was 22.6 X 10(6) [about 34.2 kilobases (kb)] and that of CELO virus DNA was 28.9 X 10(6) (about 43.7 kb). The fragments generated from B8/78 DNA by EcoRI and BamHI were physically mapped.
Isoelectric focusing: Sample pretreatment – separation – hyphenation (2009)

The three major goals of the research presented in this thesis are: • Investigation of electrophoretic sample pretreatment strategies. • Study and optimization of capillary isoelectric focusing for separation of proteins. • Hyphenation of isoelectric focusing to MS and iSPR. Chapter 2 gives an overview of the developments in cIEF over the past years including method improvements, new theoretical insights and applications. Chapter 3 describes the effect of several BGE additives on cIEF-UV repeatability and linearity and on protein signal intensity in MALDI-TOF MS. Chapter 4 describes the hyphenation of cIEF to MALDI-TOF MS via a spotting device. The development of the system as well as the effects of focusing and spotting times on separation efficiency are shown. A 3D plot is constructed from cIEF and MS data and the applicability is demonstrated for a degraded biopharmaceutical compound (glucagon). Chapter 5 is dedicated to the coupling of gel-IEF to iSPR via pressure blotting. The development of the system as well as the influence of the biological matrix on IEF separation and transfer efficiency is discussed. The applicability is demonstrated by the analysis of synovial fluid spiked with a known RA biomarker, i.e. citrullinated fibrinogen. Chapter 6 describes the use of the IonChip for salt removal from a protein sample prior to CZE separation (ITP-CZE mode) and the removal of interfering proteins from a peptide sample with subsequent CZE separation (CZE-CZE mode). Chapter 7 gives a general conclusion and recommendations for future research. Adjustments to the different systems and devices are proposed.
Industrial Warriors: South Korea’s First Generation of Industrial Workers in Post-Developmental Korea (2013)

This study analyses the skills upgrading programmes of South Korea's first generation of skilled workers, focusing on their political and social trajectories from bulwarks of the developmental regimes up until 1987, to a "labour aristocracy" of regular wo
Foxp3+ T-regulatory cells require DNA methyltransferase 1 expression to prevent development of lethal autoimmunity (2013)

Protocols to use Foxp3+ T-regulatory (Treg) cells for cellular therapy, especially postallogeneic stem cell transplantation, are currently being developed and tested by various groups. Inhibitors of DNA methyltransferase (Dnmt) enzymes have been advocated as a means to promote and stabilize Foxp3 expression in Tregs undergoing expansion in vitro before their injection in vivo. We investigated the effects of conditionally deleting two Dnmt enzymes that co-immunoprecipitated with Foxp3 in Treg isolates. Deletion of Dnmt1, but not Dnmt3a, decreased the numbers and function of peripheral Tregs and impaired conversion of conventional T cells into Foxp3+ Tregs under polarizing conditions. Importantly, mice with conditional deletion of Dnmt1 in their Tregs died of autoimmunity by 3 to 4 weeks of age unless they were rescued by perinatal transfer of wild-type Tregs. Conditional Dnmt1 deletion did not affect methylation of CpG sites within Foxp3 but decreased global DNA methylation and altered Treg expression of several hundred pro-inflammatory and other genes. Hence, Dnmt1 is necessary for maintenance of the core gene program underlying Treg development and function, and its deletion within the Treg lineage leads to lethal autoimmunity. These data suggest that caution may be warranted when considering the use of DNMT inhibitors in development of Treg-based cellular therapies.
Alternative catalysts for low-temperature CO-oxidation (1990)

MnO sub x, Ag/MnO sub x, Cu/MnO sub x, Pt/MnO sub x, Ru/MnO sub x, Au/CeO sub x, and Au/Fe2O3 were synthesized and tested for CO oxidation activity in low concentrations of stoichiometric CO and O2 at 30 to 75 C. Catalytic activity was measured for periods as long as 18000 minutes. At 75 deg Au/MnO sub x is most active sustaining nearly 100 percent CO conversion for 10000 minutes. It also retains high activity at 50 and 30 C with negligible decay in activity. A direct comparison between an unpretreated 10 percent Au/MnO sub x catalyst and an optimized 19.5 percent Pt/SnO sub 2 (pretreated) catalyst shows that the Au/MnO sub x catalyst exhibits much higher catalytic activity and far superior decay characteristics. Other catalysts including Au/CeO sub x and Au/Fe2O3 also perform well. The Cu/MnO sub x exhibits a high initial activity which decays rapidly. After the decay period the activity remains very stable making Cu/MnO sub x a potential candidate for long-term applications such as CO2 lasers in space.
Exploring difficulty in speaking tasks: An intra-task perspective (2006)

The oral presentation task has become an established format in high stakes oral testing as examining boards have come to routinely employ them in spoken language tests. This study explores how the difficulty of the Part 2 task (Individual Long Turn) in the IELTS Speaking Test can be manipulated using a framework based on the work of Skehan (1998), while working within the socio-cognitive perspective of test validation. The identification of a set of four equivalent tasks was undertaken in three phases. One of these tasks was left unaltered; the other three were manipulated along three variables: planning time, response time and scaffolded support. In the final phase of the study, 74 language students, at a range of ability levels, performed all four versions of the tasks and completed a brief cognitive processing questionnaire after each performance. The resulting audio files were then rated by two IELTS trained examiners working independently of each other using the current IELTS Speaking criteria. The questionnaire data were analysed in order to establish any differences in cognitive processing when performing the different task versions. Results from the score data suggest that while the original un-manipulated version tends to result in the highest scores, there are significant differences to be found in the responses of three ability groups to the four tasks, indicating that task difficulty may well be affected differently for test candidates of different ability. These differences were reflected in the findings from the questionnaire analysis. The implications of these findings for teachers, test developers, test validators and researchers are discussed.
Pregnancy and fetal outcomes of symptoms of sleep-disordered breathing (2010)

The physiological changes of pregnancy may predispose females to develop sleep-disordered breathing (SDB) or protect against it. Studies evaluating outcomes of SDB symptoms in pregnancy are scarce. The goal of this study was to evaluate the prevalence of SDB symptoms in pregnancy and their relationship with pregnancy and neonatal outcomes. A cross-sectional survey of randomly selected immediate postpartum females was performed using the multivariable apnoea prediction index. Record review, including demographics and medical history, was performed. Main outcome measures included pregnancy and neonatal outcomes. 1,000 subjects were recruited. Mean± sd age was 29.1±6.1 yrs. Factors used in the regression analysis included age, body mass index, diabetes, chronic hypertension, multifetal gestations, smoking and renal disease. Snoring was present in 35.1% of subjects. Symptoms of SDB were associated with a higher likelihood of pregnancy-induced hypertension and pre-eclampsia (adjusted OR 2.3, 95% CI 1.4–4.0), gestational diabetes (adjusted OR 2.1, 95% CI 1.3–3.4) and unplanned Caesarean deliveries (adjusted OR 2.1, 95% CI 1.4–3.2) after multivariable regression analysis. Gasping may have been associated with a higher likelihood of preterm delivery, after adjusting for age and multifetal pregnancies (adjusted OR 1.8, 95% CI 1.1–3.2) but this association appeared to be mediated by pre-eclampsia. Symptoms of SDB are common in pregnancy and associated with a higher likelihood of gestational hypertensive disorders, gestational diabetes and unplanned Caesarean deliveries.
Rigid body segmentation and shape description from dense optical flow under weak perspective (2002)

We present an algorithm for identifying and tracking independently moving rigid objects from optical flow. The proposed method uses the fact that each distinct object has a unique epipolar constraint associated with its motion. This is in contrast to using local optical flow information for segmentation. Thus motion discontinuities based on self-occlusion are distinguished from those due to separate objects. The use of epipolar geometry allows for the determination of individual motion parameters for each object as well as the recovery of relative depth for each point on the object. The segmentation problem is formulated as a scene partitioning problem and a statistic-based algorithm which uses only nearest neighbor interactions and a finite number of iterations is developed. A Kalman filter based approach is used for tracking motion parameters with time. The algorithm assumes an affine camera where perspective effects are limited to changes in overall scale. No camera calibration parameters are required.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The effect of the addition of proteases and glucanases during yeast autolysis on the production and properties of yeast extracts (2001)

Yeast extracts (YE) were produced with the addition of proteases or glucanases during bakers' yeast (Saccharomyces cerevisiae) autolysis. Chemical composition, physical properties, and biological value of the YE were examined. Proteases had the highest impact on the turbidity and filterability of YE. All 11 proteases and two glucanases increased YE yields (% yeast solids solubilized) obtained from heated (80 degrees C/15 min) bakers' yeast creams (BYC). However, when proteases were added to native (unheated) BYC during autolysis, few increased YE yields, with papain being the most effective. The increased yields were generally related to increased levels of total nitrogen (TN) and alpha-amino nitrogen (alpha-AN) in the YE. Media were supplemented with the various yeast extracts, and the highest growth rates (mumax) and biomass values (ODmax) of Lactobacillus acidophilus were noted. The best growth was obtained with YE produced with native BYC treated with a fungal protease, and results of this study show that some enzymes could be used to produce improved YE for microbiological media.
Pathogen Inactivation (2007)

Remarkable improvements have been made in blood safety since the onset of the HIV epidemic. However, the current paradigm does not prevent all transfusion-transmitted infections and is reactive to new agents, thus accepting that some patients may be harmed before preventive measures are introduced. Several methods are now available that selectively damage DNA and RNA, thus inactivating pathogens contaminating blood components while not damaging the cells or plasma proteins of the blood component. Clinical trials have been completed and pathogen-inactivated platelets and plasma are widely used in Europe. A recent consensus conference recommended implementation of pathogen inactivation when a feasible and fe method is available that inactivates a broad spectrum of pathogens. The shortcomings of our present paradigm for preventing transfusion-transmitted diseases are described, along with a summary of the status of pathogen inactivation.
Pheno- or genotype for the CYP2C19 drug metabolism polymorphism: the influence of disease. (2008)

A genotyping approach for pharmacogenes such as CYP2C19 is often advocated to "personalise therapy", since individuals with the homozygous null allele genotype are poor metabolisers (PM) of many drugs. The genotype-phenotype relationship is a validated approach for S-mephenytoin, proguanil and omeprazole in young, healthy populations. However, this relationship may not be valid in patients with diseases such as cancer and congestive heart failure, or in old age. The high phenotypic discordance in the genotypic EM group means that in many clinical situations the true number of CYP2C19 poor metabolisers may be under-estimated if only genotypic approaches are used.
Role of Surface Hydroxyl Species in Copper-Catalyzed Hydrogenation of Ketones (2018)

A comprehensive, coverage-dependent mean-field microkinetic model is developed for the hydrogenation of carbonyl compounds on Cu(111). In the model, hydrogenation by surface hydrogen, surface hydroxyl species, and adsorbed water molecules is considered, including a reaction pathway via keto–enol tautomerization. The model parameters were calculated by VdW-DF2 density functional theory and account for inter- and intraspecies repulsion. Accounting for these coverage effects changes the surface from being completely covered with 25% oxygen atoms and 75% hydroxyl groups to a surface with 65% free sites. Including coverage effects also surprisingly increases the calculated turnover frequency from 6 × 10–5 to 2 × 10–3 s–1. In the dominant reaction path, the carbonyl group is hydrogenated to an alkoxy intermediate by surface hydrogen, followed by a proton transfer from either a surface hydroxyl species or an adsorbed water molecule to form the alcohol product. The addition of small amounts of water suffices to open this pathway. The pathway in which acetone is converted to 2-hydroxypropylene via keto–enol tautomerization is kinetically irrelevant under the considered conditions. Regeneration of the hydroxyl groups is the rate-controlling step in the mechanism, suggesting an alternative role for the reducible oxide promoters which are often encountered for Cu-based carbonyl hydrogenation catalysts.
Design, Synthesis, and Antibacterial Activity of Demethylvancomycin Analogues against Drug‐Resistant Bacteria (2013)

Five novel N-substituted demethylvancomycin derivatives were rationally designed and synthesized by using a structure-based approach. The in vitro antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA), gentamicin-resistant Enterococcus faecalis (GRE), methicillin-resistant Streptococcus pneumoniae (MRS), and vancomycin-resistant Enterococcus faecalis (VRE) were evaluated. One of the compounds, N-(6-phenylheptyl)demethylvancomycin (12 a), was found to exhibit more potent antibacterial activity than vancomycin and demethylvancomycin. Compound 12 a was also found to be ~18-fold more efficacious than vancomycin against MRSA; however, the two compounds were found to have similar efficacy against MRS. Furthermore, compound 12 a exhibited a favorable pharmacokinetic profile with a half-life of 5.11±0.52 h, which is longer than that of vancomycin (4.3±1.9 h). These results suggest that 12 a is a promising antibacterial drug candidate for further preclinical evaluation.
HDL Library of Processing Units for an Automatic LDPC Decoder Design (2006)

In this paper we propose an efficient and generic HDL library of processing units which are the key elements of a modular low-density parity check (LDPC) decoder design approach. General purpose, low complexity and high throughput bit node and check functional models are developed. Both full serial and parallel architectures are considered. Also, it is described an automatic HDL code generator for the proposed processing units using Matlab language and synthesis results for a Xilinx FPGA device are documented
Free Radical Lipid Peroxidation: Mechanisms and Analysis (2011)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTFree Radical Lipid Peroxidation: Mechanisms and AnalysisHuiyong Yin†‡, Libin Xu†, and Ned A. Porter*†View Author Information† Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, United States‡ Departments of Medicine and Pharmacology, Division of Clinical Pharmacology, Vanderbilt School of Medicine, Nashville, Tennessee 37232, United StatesE-mail: [email protected]Cite this: Chem. Rev. 2011, 111, 10, 5944–5972Publication Date (Web):August 23, 2011Publication History Received18 March 2011Published online23 August 2011Published inissue 12 October 2011https://pubs.acs.org/doi/10.1021/cr200084zhttps://doi.org/10.1021/cr200084zreview-articleACS PublicationsCopyright © 2011 American Chemical SocietyRequest reuse permissionsArticle Views20137Altmetric-Citations1255LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Free radicals,Lipids,Oxidation,Oxides,Oxygen Get e-Alerts
Spectral Leakage-Driven Loopback Scheme for Prediction of Mixed-Signal Circuit Specifications (2018)

The rising cost of production testing for a system-on-a-chip (SoC) is one of the crucial matters to chip makers, due to long test time and costly automated-test-equipment. This paper proposes a spectral leakage-driven built-in self-test (BIST) scheme to precisely predict the nonlinearity of mixed-signal circuits in the loopback mode, thereby accomplishing cost-effectiveness (compared to previous BIST-based works). A digitally synthesized single-tone sinusoidal stimulus used for conventional harmonic testing is incoherently sampled by a device under test (DUT). The DUT output signal exhibits the correlation between the DUT harmonics and the spectral leakage introduced by the incoherent sampling. The DUT output signal is then fed to another DUT through a loopback path, so that the harmonics of a pair of DUTs are correlated with the spectral leakage on the loopback response; the magnitude of the spectral leakage is considered as a weighting factor on the harmonic magnitude of those DUTs. The correlation is quantitatively modeled as characteristic equations in (15), and postprocessing predicts the harmonics of the two individual DUTs, by simultaneously solving the characteristic equations using on-chip DSP core available in an SoC. Simulation and hardware measurements validated that this paper can be practically used for production testing by showing less than 0.3 and 0.6 dB of the prediction errors, respectively.