gebhart/patrepeval huggingface dataset:
The ubiquitin‐protein ligase Nedd4 targets Notch1 in skeletal muscle and distinguishes the subset of atrophies caused by reduced muscle tension (2006)

Ubiquitination-dependent proteolysis is a fundamental process underlying skeletal muscle atrophy. Thus, the role of ubiquitin ligases is of great interest. There are no focused studies in muscle on the ubiquitin ligase Nedd4. We first confirmed increased mRNA expression in rat soleus muscles due to 1-14 days of hind limb unloading. Nedd4 protein localized to the sarcolemmal region of muscle fibers. Hind limb unloading, sciatic nerve denervation, starvation, and diabetes led to atrophy of soleus, plantaris, and gastrocnemius muscles, but only unloaded and denervated muscles showed a marked increase in Nedd4 protein expression. This increase was strongly correlated with decreased Notch1 expression, a known target of Nedd4 in other cell types. Overexpression of dominant negative Nedd4 in soleus muscles completely reversed the unloading-induced decrease of Notch1 expression, indicating that Nedd4 is required for Notch1 inactivation. Overexpression of wild-type Nedd4 in soleus muscles of weight bearing rats caused a decrease in Notch1 protein, indicating that Nedd4 is sufficient for Notch1 down-regulation. To further show that Notch1 is a Nedd4 substrate in muscle, conditional overexpression of Nedd4 in C2C12 myotubes induced ubiquitination of Notch1. This is the first finding of a Nedd4 substrate in muscle and of an ubiquitin ligase, the activity of which distinguishes disuse from cachexia atrophy.
Bacterial Flora of the Vagina: Quantitative Study (1984)

Quantitative analysis of cultures of the vaginal flora was performed on 118 specimens from 68 women of reproductive age. Aerobic bacteria in concentrations greater than 10(5) organisms/g were recovered in all specimens. The mean concentration of aerobes was 10(8.1) organisms/g, and lactobacilli were the most common isolates. Anaerobes were recovered from 86% of the specimens, and the mean concentration of these organisms, when present, was 10(8.6) organisms/g. The most commonly occurring anaerobes were peptococci and anaerobic gram-positive bacilli. Analysis of specimens obtained sequentially during a menstrual cycle showed significant qualitative and quantitative changes. Cervical cultures showed bacteriologic patterns that were similar to those noted with vaginal cultures. However, duplicate samplings of the cervix and vagina in individual patients showed substantial differences in the bacteria recovered. These observations suggest that the vaginal flora is a dynamic ecosystem that is subject to change and that the cervix represents a unique ecologic niche.
Image reconstruction from zero crossings (1986)

This study is concerned with the information in zero crossings (ZC) of images. Logan's conditions, specifying when a one-dimensional signal may be recovered (within a multiplicative constant) from its ZC, are extended for various cases of two-dimensional signals. An algorithm is implemented in reconstruction of context-free band-pass images. It is also successfully applied in reconstruction of contextual images by first dissecting the image into appropriate bandpass-band-limited two-dimensional signals. In almost all cases of spectrum confined to less than an octave in one dimension, the reconstruction algorithm converges, whereas it does not converge for any signal exceeding one octave in bandwidth. This paper substantiates the proposition that images are well represented by the partial information confined to ZC.
Temperature Control Apparatus (1929)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Lorenzo A. Richards, "Temperature Control Apparatus," J. Opt. Soc. Am. 18, 131-137 (1929) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
8. Inversion of Controlled-Source Electromagnetic Data (1988)

Given a set of geophysical measurements, we want to determine all information possible about the geologic structure that gave rise to the data. There are two aspects. First, because we use electrical and electromagnetic (EM) methods of exploration we want to determine the geoelectric structure of the earth; i.e., the conductivity and/or the permittivity as a function of depth and lateral distance. If we can do this, then the second aspect will relate the geoelectric structure to the geologic structure. In some cases, a one-to-one relationship exists between the two; in others, there may be only a partial but useful overlap. This chapter addresses the first aspect—determining the geoelectrical structure based on controlled-source electromagnetic (CSEM) data. The earliest inversion methods can be described as trial-and-error exercises. Starting with a general model, such as a layered earth or a thin dike, and then adjusting the parameters of the model we achieve a best-fit to the data. The parameters in this case are the physical dimensions and conductivities of the structures. Basically the exercise consists of running a number of forward models, and systematically adjusting the important parameters until the response matches the observed data within the noise level of the measurements. Model-fitting by trial-and-error is still the most common interpretation technique for multidimensional models, but there are problems with the approach: 1. There is an a priori restriction to classes of models for which a relatively fast forward solution exists. 2. Because relatively simple models can have several parameters, some of which may be strongly correlated, the trial-and-error process may involve a prohibitive amount of time. 3. Fitting an assumed physical model to observed data involves a high degree of subjectivity. It is important to estimate confidence intervals for the various parameters and for other statistical measures that provide insight into how appropriate the model is. The trial-and- error approach does not provide this information.
Biologic effects of leukocytes present in transfused cellular blood products (1994)

A considerable literature has accumulated over the past decade indicating that leukocytes present in allogeneic cellular blood components, intended for transfusion, are associated with adverse effects to the recipient. These include the development of febrile transfusion reactions, graft-versus-host disease, alloimmunization to leukocyte antigens, and the immunomodulatory effects that might influence the prognosis of patients with a malignancy. Moreover, it has become evident that such leukocytes may be the vector of infectious agents such as CMV, HTLV-I/II, and EBV as well as other viruses. An interesting development that has occurred coincidentally in transfusion medicine is that agencies responsible for the provision of blood products are being designated manufacturers of biologicals. The trend among manufacturers of biologicals is to try to produce pure products to provide for the specific therapeutic need of patients. Thus, with the realization that allogeneic leukocytes and their products may have adverse biologic activities, there is increasing pressure from various sources for the reduction of the leukocyte content in allogeneic blood components to minimize the occurrence of their adverse effects. Although the threshold leukocyte number below which these effects might no longer occur is still to be determined, a 2 to 3 log10 leukocyte reduction, provided by the currently available commercial leukocyte filters, has been shown to reduce the frequency of many of such reactions. On the other hand, the immunosuppressive effects produced by the infusion of allogeneic leukocytes might be beneficial for some patients, ie, for the maintenance of kidney allografts, in possibly reducing the relapse rate in patients with inflammatory bowel diseases, and in ameliorating recurrent spontaneous abortion. Moreover, therapeutic granulocyte transfusions may be of benefit in certain well-defined categories of patients infected with bacteria, yeast, and/or fungi. These include neonates with bacterial sepsis associated with bone marrow failure as well as severely neutropenic leukemic patients with an infection unresponsive to appropriate and specific antibiotic therapy. Many of the results obtained with the use of leukocyte depletion filters are tantalizing, but the actual clinical benefit of leukodepletion has not been established in most instances, because much of the available data are retrospective or from uncontrolled clinical trials. Moreover, issues of cost-effectiveness and quality control have not been considered adequately. Properly designed prospective clinical trials are essential to provide data with which to answer such questions and also to help define the optimal conditions required for the preparation of blood components ultimately destined for clinical use. Only with the availability of such data can sound decisions be made about the clinical value of leukodepletion.
Physiologically Active Compounds. V.<sup>1a,b</sup> Aminothiol Esters of Substituted Acetic, Chloroacetic, Benzilic, and Related Acids<sup>2</sup> (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhysiologically Active Compounds. V.1a,b Aminothiol Esters of Substituted Acetic, Chloroacetic, Benzilic, and Related Acids2C. A. Buehler, Hilton A. Smith, Allen C. Kryger, Roy L. Wells, and Shelby F. ThamesCite this: J. Med. Chem. 1963, 6, 3, 230–233Publication Date (Print):May 1, 1963Publication History Published online1 May 2002Published inissue 1 May 1963https://pubs.acs.org/doi/10.1021/jm00339a003https://doi.org/10.1021/jm00339a003research-articleACS PublicationsRequest reuse permissionsArticle Views146Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Immunization with <i>Pneumocystis carinii</i> gpA Is Immunogenic but Not Protective in a Mouse Model of <i>P. carinii</i> Pneumonia (1998)

Immunization with whole Pneumocystis carinii has been shown to protect mice from the development of P. carinii pneumonia (PCP) when they are subsequently immunosuppressed and challenged with viable organisms. To determine whether these results could be duplicated by using a subunit vaccine, we examined the immunogenicity and efficacy of an immunization strategy based on P. carinii gpA. This antigen was chosen for study because passive immunoprophylaxis, based on gpA, has been shown to be partially protective in various animal models of infection. Immunization with gpA produced an anti-gpA specific antibody response comparable to that resulting from immunization with whole organisms. However, in contrast to immunization with whole P. carinii, which was protective, immunization with gpA did not protect T-cell-depleted mice from the development of PCP. These studies suggest that other antigens in addition to gpA need to be evaluated for their role in protective immunity against P. carinii.
Talking Face Generation by Conditional Recurrent Adversarial Network (2018)

Given an arbitrary face image and an arbitrary speech clip, the proposed work attempts to generating the talking face video with accurate lip synchronization while maintaining smooth transition of both lip and facial movement over the entire video clip. Existing works either do not consider temporal dependency on face images across different video frames thus easily yielding noticeable/abrupt facial and lip movement or are only limited to the generation of talking face video for a specific person thus lacking generalization capacity. We propose a novel conditional video generation network where the audio input is treated as a condition for the recurrent adversarial network such that temporal dependency is incorporated to realize smooth transition for the lip and facial movement. In addition, we deploy a multi-task adversarial training scheme in the context of video generation to improve both photo-realism and the accuracy for lip synchronization. Finally, based on the phoneme distribution information extracted from the audio clip, we develop a sample selection method that effectively reduces the size of the training dataset without sacrificing the quality of the generated video. Extensive experiments on both controlled and uncontrolled datasets demonstrate the superiority of the proposed approach in terms of visual quality, lip sync accuracy, and smooth transition of lip and facial movement, as compared to the state-of-the-art.
CpG methylation regulates allelic expression of GDF5 by modulating binding of SP1 and SP3 repressor proteins to the osteoarthritis susceptibility SNP rs143383 (2014)

GDF5 encodes an extracellular signalling molecule that is essential for normal skeletal development. The rs144383 C to T SNP located in the 5'UTR of this gene is functional and has a pleiotropic effect on the musculoskeletal system, being a risk factor for knee-osteoarthritis (OA), congenital hip dysplasia, lumbar disc degeneration and Achilles tendon pathology. rs143383 exerts a joint-wide effect on GDF5 expression, with expression of the OA-associated T allele being significantly reduced relative to the C allele, termed allelic expression imbalance. We have previously reported that the GDF5 locus is subject to DNA methylation and that allelic imbalance of rs143383 is mediated by SP1, SP3 and DEAF1 transcriptional repressors. In this study, we have assayed GDF5 methylation in normal and osteoarthritic cartilage, and investigated the effect of methylation on the allelic imbalance of rs143383. We observed demethylation of the GDF5 5'UTR in OA knee cartilage relative to both OA (p = 0.009) and non-OA (p = 0.001) hip cartilage, with the most significant demethylation observed at the highly conserved +37 CpG site located 4 bp upstream of rs143383. Methylation modulates the level and direction of allelic imbalance of rs143383, with methylation of the +37 CpG dinucleotide within the SP1/SP3 binding site having an allele-specific effect on SP1 and SP3 binding. Furthermore, methylation attenuated the repressive effects of SP1, SP3 and DEAF1 on GDF5 promoter activity. This data suggest that the differential methylation of the +37 CpG site between osteoarthritic hip and knee cartilage may be responsible for the knee-specific effect of rs143383 on OA susceptibility.
Prolyl Hydroxylase EGLN3 Regulates Skeletal Myoblast Differentiation through an NF-κB-dependent Pathway (2010)

The egg-laying abnormal-9 (EGLN) prolyl hydroxylases have been shown to regulate the stability and thereby the activity of the alpha subunits of hypoxia-inducible factor (HIF) through its ability to catalyze their hydroxylation. We have previously shown that EGLN3 promotes differentiation of C2C12 skeletal myoblasts. However, the mechanism underlying this effect remains to be fully elucidated. Here, we report that exposure of C2C12 cells to dimethyl oxalylglycine (DMOG), desferrioxamine, and hypoxia, all inhibitors of prolyl hydroxylase activity, led to repression of C2C12 myogenic differentiation. Inactivation of HIF by expression of a HIF dominant-negative mutant or deletion of HIF-1alpha by RNA interference did not affect the inhibitory effect of DMOG, suggesting that the effect of DMOG is HIF-independent. Pharmacologic inactivation of EGLN3 hydroxylase resulted in activation of the canonical NF-kappaB pathway. The inhibitory effect of DMOG on myogenic differentiation was markedly impaired in C2C12 cells expressing a dominant-negative mutant of IkappaBalpha. Exogenous expression of wild-type EGLN3, but not its catalytically inactive mutant, significantly inhibited NF-kappaB activation induced by overexpressed TRAF2 or IkappaB kinase 2. In contrast, deletion of EGLN3 by small interfering RNAs led to activation of NF-kappaB. These data suggest that EGLN3 is a negative regulator of NF-kappaB, and its prolyl hydroxylase activity is required for this effect. Furthermore, wild-type EGLN3, but not its catalytically inactive mutant, potentiated myogenic differentiation. This study demonstrates a novel role for EGLN3 in the regulation of NF-kappaB and suggests that it is involved in mediating myogenic differentiation, which is HIF-independent.
Right Cerebral Dominance in Spatial Attention (1987)

Tasks based on visuomotor scanning and tactile exploration were used to quantitate neglect behavior in patients with unilateral brain damage and in normal control subjects. The results confirm previous observations that contralateral neglect is markedly more severe following right-hemisphere injury and that it is independent of the modality of sensory input or motor output. In addition, patients with right-hemisphere injury also showed multimodal neglect for targets in the hemisphere ipsilateral to the brain lesion. The emergence of both contralateral and ipsilateral neglect in these patients strongly supports a model of right-hemispheric dominance for the distribution of attention within the extrapersonal space.
Epigallocatechin, a Green Tea Polyphenol, Attenuates Myocardial Ischemia Reperfusion Injury in Rats (2004)

Epigallocatechin-3-gallate (EGCG) is the most prominent catechin in green tea. EGCG has been shown to modulate numerous molecular targets in the setting of inflammation and cancer. These molecular targets have also been demonstrated to be important participants in reperfusion injury, hence this study examines the effects of EGCG in myocardial reperfusion injury. Male Wistar rats were subjected to myocardial ischemia (30 min) and reperfusion (up to 2 h). Rats were treated with EGCG (10 mg/kg intravenously) or with vehicle at the end of the ischemia period followed by a continuous infusion (EGCG 10 mg/kg/h) during the reperfusion period. In vehicle-treated rats, extensive myocardial injury was associated with tissue neutrophil infiltration as evaluated by myeloperoxidase activity, and elevated levels of plasma creatine phosphokinase. Vehicle-treated rats also demonstrated increased plasma levels of interleukin-6. These events were associated with cytosol degradation of inhibitor kappaB-alpha, activation of IkappaB kinase, phosphorylation of c-Jun, and subsequent activation of nuclear factor-kappaB and activator protein-1 in the infarcted heart. In vivo treatment with EGCG reduced myocardial damage and myeloperoxidase activity. Plasma IL-6 and creatine phosphokinase levels were decreased after EGCG administration. This beneficial effect of EGCG was associated with reduction of nuclear factor-kB and activator protein-1 DNA binding. The results of this study suggest that EGCG is beneficial for the treatment of reperfusion-induced myocardial damage by inhibition of the NF-kappaB and AP-1 pathway.
A Large Toxin from Pathogenic <i>Escherichia coli</i> Strains That Inhibits Lymphocyte Activation (2000)

The mechanisms by which bacteria resist cell-mediated immune responses to cause chronic infections are largely unknown. We report the identification of a large gene present in enteropathogenic strains of Escherichia coli (EPEC) that encodes a toxin that specifically inhibits lymphocyte proliferation and interleukin-2 (IL-2), IL-4, and gamma interferon production in response to a variety of stimuli. Lymphostatin, the product of this gene, is predicted to be 366 kDa and shares significant homology with the catalytic domains of the large clostridial cytotoxins. A mutant EPEC strain that has a disruption in this gene lacks the ability to inhibit lymphokine production and lymphocyte proliferation. Enterohemorrhagic E. coli strains of serotype O157:H7 possess a similar gene located on a large plasmid. Loss of the plasmid is associated with loss of the ability to inhibit IL-2 expression while transfer of the plasmid to a nonpathogenic strain of E. coli is associated with gain of this activity. Among 89 strains of E. coli and related bacteria tested, lifA sequences were detected exclusively in strains capable of attaching and effacing activity. Lymphostatin represents a new class of large bacterial toxins that blocks lymphocyte activation.
Methylphenidate for the Treatment of Apathy in Alzheimer Disease (2008)

Apathy is a common behavioral symptom of Alzheimer's disease (AD), being present in up to 70% of patients. Apathy in AD and non-AD populations has been associated with dysfunction in the dopaminergic brain reward system, suggesting that pharmacotherapeutic targeting of this system may be an effective treatment for apathy in AD. We therefore performed a randomized, double-blind, placebo-controlled crossover trial of methylphenidate in a sample of 13 apathetic AD patients (6 men, 7 women; age mean 77.9 years [SD, 7.8 years]; Mini Mental Status Examination score, 19.9 [SD, 4.7]). Patients were treated with methylphenidate (10 mg PO twice a day) or an identical placebo in two 2-week phases separated by a 1-week placebo washout. All patients participated in a dextroamphetamine challenge test (one 10-mg oral dose) before treatment with methylphenidate to gauge the functional integrity of the dopamine brain reward system. Overall, patients demonstrated greater improvement with methylphenidate compared with placebo according to Apathy Evaluation Scale total change scores (end of treatment - baseline: Wilcoxon Z = -2.00; P = 0.047). However, a significantly greater proportion of patients experienced at least 1 adverse event with methylphenidate compared with placebo (3 vs 1; chi = 4.33, P = 0.038). Two patients experienced serious adverse events with methylphenidate, consisting of delusions, agitation, anger, irritability, and insomnia, which resolved upon discontinuation of the medication. Response to methylphenidate was associated with increases in inattention on a continuous performance task after dextroamphetamine challenge. Psychostimulants may be effective in treating features of apathy in AD, and dopaminergic changes may predict response.