gebhart/patrepeval huggingface dataset:
Biological Effects and Cellular Uptake of c-<i>myc</i>Antisense Oligonucleotides and Their Cationic Liposome Complexes (1998)

The biological effects and cellular uptake of human c-myc antisense oligonucleotides and their liposome complexes were investigated in vitro using human promonocytic leukemia U937 cells. Antisense phosphorothioate oligonucleotides (S-Oligo) significantly inhibited the growth of U937 cells in a dose-dependent manner. However, no significant effect on cell proliferation was observed with unmodified phosphodiester (P-Oligo) and partially phosphorothioated (PS3-Oligo) oligonucleotides with an antisense sequence and S-Oligo with sense and G-quartet control sequences. In cellular uptake experiments, radiolabeled S-Oligo was taken up by U937 cells more than P-Oligo and PS3-Oligo. Similar results were obtained in mouse peritoneal macrophages used for comparison. Confocal microscopic studies demonstrated a significant distribution of FITC-labeled oligonucleotides on the cell surface and in the cytoplasm in a punctate pattern, but not in the nucleus. When complexed with cationic liposomes, cellular uptake of FITC-labeled P-Oligo or S-Oligo was significantly increased and the fluorescence was located mainly in the nucleus, indicating that the uptake and intracellular pharmacokinetics of both oligonucleotides can be modified by complexation. An inhibitory effect of the complexes was observed at a dose which is ineffective in the case of the oligonucleotides alone. However, this effect was also associated with cytotoxicity of the cationic liposomes, suggesting that optimization of this formulation will be necessary to achieve a more efficient delivery of the oligonucleotides to U937 cells.
Beneficial Metabolic Effects of 2′,3′,5′-tri-acetyl-N6- (3-Hydroxylaniline) Adenosine in the Liver and Plasma of Hyperlipidemic Hamsters (2012)

This work demonstrates the promise of applying (1)H NMR metabonomics to evaluate the beneficial effects of WS070117 which may be a good drug candidate for hyperlipidemia.
Preparation of 2,3-Dihydroxybenzoic Acid (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of 2,3-Dihydroxybenzoic AcidJames Cason and Guy Oren Dyke Jr.Cite this: J. Am. Chem. Soc. 1950, 72, 1, 621–622Publication Date (Print):January 1, 1950Publication History Published online1 May 2002Published inissue 1 January 1950https://pubs.acs.org/doi/10.1021/ja01157a501https://doi.org/10.1021/ja01157a501research-articleACS PublicationsRequest reuse permissionsArticle Views322Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The use of 16S rDNA sequence analysis to investigate the phylogeny of Leptospiraceae and related spirochaetes (1993)

The 16S rDNA sequences from 15 Leptospiraceae were determined by automated PCR-directed cycle sequencing. Nucleotide comparisons, including those from published sequences for Leptospira canicola Moulton and Serpulina spp., were used to construct phylogenetic trees. Serpulina hyodysenteriae and S. innocens were related to each other but were distinct from the Leptospiraceae comprising Leptospira parva incertae sedis (Turneria parva H), Leptonema illini and Leptospira spp. The pathogenic and the saprophytic leptospires were distinct and separated from each other. Leptospira inadai occupied an intermediate position between the two forms. The pathogens formed three groups. Group I was represented by L. interrogans sensu stricto and L. kirschneri, Group II by L. weilii, L. borgpetersenii and L. santarosai, and Group III comprised L. noguchii and L. meyeri. The saprophytic species, L. wolbachii and L. biflexa sensu stricto shared about 99% sequence similarity. The freshwater isolates were distinct from the marine isolate L. biflexa sensu lato ancona Ancona Porto.
Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads (1994)

Journal Article Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads Get access Yao-Guang Liu, Yao-Guang Liu * Mitsui Plant Biotechnology Research Institute, RITE Tsukuba # 1 LaboratoryTCI-D21, Sengen 2-1-6, Tsukuba 305, Japan * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Robert F. Whittier Robert F. Whittier Mitsui Plant Biotechnology Research Institute, RITE Tsukuba # 1 LaboratoryTCI-D21, Sengen 2-1-6, Tsukuba 305, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 22, Issue 11, 11 June 1994, Pages 2168–2169, https://doi.org/10.1093/nar/22.11.2168 Published: 11 June 1994 Article history Received: 18 April 1994 Accepted: 05 May 1994 Published: 11 June 1994
Gulf and Angola (1972)

On April 19 of this year the President and Fellows of Harvard College announced their intention to “send a representative to Angola to provide us first-hand information on Gulf's performance, the problems it faces, and opportunities for constructive new steps, and thereby to help us determine our future actions as a responsible Gulf shareholder.”
Laser-induced photoacoustics influenced by single-cycle terahertz radiation (2010)

Laser-induced plasma acoustic waves are enhanced under the illumination of single-cycle terahertz (THz) radiation, making THz-enhanced acoustics (TEA) a useful method for THz wave detection. During a single-cycle THz pulse with its peak field of 100 kV/cm, a pressure enhancement of 10% is observed throughout the acoustic spectrum up to 140 kHz, and the TEA signal is found to increase linearly with THz wave intensity. By using dual-color laser excitation to manipulate free electron drift, it is possible to modulate the enhanced acoustic signal and recover a coherent THz time-domain waveform by simply "listening" to the plasma.
Convolutional Neural Networks based Click-Through Rate Prediction with Multiple Feature Sequences (2018)

Convolutional Neural Network (CNN) achieved satisfying performance in click-through rate (CTR) prediction in recent studies. Since features used in CTR prediction have no meaningful sequence in nature, the features can be arranged in any order. As CNN learns the local information of a sample, the feature sequence may influence its performance significantly. However, this problem has not been fully investigated. This paper firstly investigates whether and how the feature sequence affects the performance of the CNN-based CTR prediction method. As the data distribution of CTR prediction changes with time, the best current sequence may not be suitable for future data. Two multi-sequence models are proposed to learn the information provided by different sequences. The first model learns all sequences using a single feature learning module, while each sequence is learnt individually by a feature learning module in the second one. Moreover, a method of generating a set of embedding sequences which aims to consider the combined influence of all feature pairs on feature learning is also introduced. The experiments are conducted to demonstrate the effectiveness and stability of our proposed models in the offline and online environment on both the benchmark Avazu dataset and a real commercial dataset.
Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism (2012)

To identify new genes that are important in fat metabolism, we utilized the Lexicon-Genentech knockout database of genes encoding transmembrane and secreted factors and whole murine genome transcriptional profiling data that we generated for 3T3-L1 in vitro adipogenesis. Cross-referencing null models evidencing metabolic phenotypes with genes induced in adipogenesis led to identification of a new gene, which we named RIFL (refeeding induced fat and liver). RIFL-null mice have serum triglyceride levels approximately one-third of wild type. RIFL transcript is induced >100-fold during 3T3-L1 adipogenesis and is also increased markedly during adipogenesis of murine and human primary preadipocytes. siRNA-mediated knockdown of RIFL during 3T3-L1 adipogenesis results in an ~35% decrease in adipocyte triglyceride content. Murine RIFL transcript is highly enriched in white and brown adipose tissue and liver. Fractionation of WAT reveals that RIFL transcript is exclusive to adipocytes with a lack of expression in stromal-vascular cells. Nutritional and hormonal studies are consistent with a prolipogenic function for RIFL. There is evidence of an approximately eightfold increase in RIFL transcript level in WAT in ob/ob mice compared with wild-type mice. RIFL transcript level in WAT and liver is increased ~80- and 12-fold, respectively, following refeeding of fasted mice. Treatment of 3T3-L1 adipocytes with insulin increases RIFL transcript ≤35-fold, whereas agents that stimulate lipolysis downregulate RIFL. Interestingly, the 198-amino acid RIFL protein is predicted to be secreted and shows ~30% overall conservation with the NH(2)-terminal half of angiopoietin-like 3, a liver-secreted protein that impacts lipid metabolism. In summary, our data suggest that RIFL is an important new regulator of lipid metabolism.
Study of metabolic network of <i>Cupriavidus necator</i> DSM 545 growing on glycerol by applying elementary flux modes and yield space analysis (2014)

A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obtained by the program Metatool and analyzed by using yield space analysis. Four sets of elementary modes were obtained, depending on whether the pair NAD/NADH or FAD/FADH2 contributes to the reaction of glycerol-3-phosphate dehydrogenase (GLY-3-P DH), and whether 6-phosphogluconate dehydrogenase (6-PG DH) is present or not. Established metabolic network and the related system of equations provide multiple solutions for the simultaneous synthesis of PHB and biomass; this number of solutions can be further increased if NAD/NADH or FAD/FADH2 were assumed to contribute in the reaction of GLY-3-P DH. As a major outcome, it was demonstrated that experimentally determined yields for biomass and PHB with respect to glycerol fit well to the values obtained in silico when the Entner-Doudoroff pathway (ED) dominates over the glycolytic pathway; this is also the case if the Embden-Meyerhof-Parnas pathway dominates over the ED.
Development of optical current transformers and application to fault location systems for substations (1993)

The authors report on an optical current transformer (CT) based on the principle of the Faraday effect. The information measured by the optical CT is transmitted to the ground side through optical fibers contained in an insulator. An application of the optical CT, a new fault location system, is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Renal Recovery after Severe Acute Renal Failure (2006)

The majority of severe ARF patients who survive their acute illness are independent of RRT by 90 days. Male sex and a diagnosis of septic shock are independently associated with recovery while a greater co-morbidity score and a higher serum creatinine prior to RRT are predictive of non-recovery.
EXPRESSION OF CYCLOOXYGENASE-2 IN CHOROIDAL NEOVASCULAR MEMBRANES FROM AGE-RELATED MACULAR DEGENERATION PATIENTS (2009)

The expression of COX-2 in human choroidal neovascular membranes suggests a possible role for this modulator in age-related macular degeneration pathogenesis. The age-dependent expression observed is novel and warrants further investigation.
Pharmacokinetic Evaluation of a DSPE-PEG2000 Micellar Formulation of Ridaforolimus in Rat (2012)

The rapamycin analog, ridaforolimus, has demonstrated potent anti-proliferative effects in cancer treatment, and it currently is being evaluated in a range of clinical cancer studies. Ridaforolimus is an extremely lipophilic compound with limited aqueous solubility, which may benefit from formulation with polymeric micelles. Herein, we report the encapsulation of ridaforolimus in 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) (DSPE-PEG2000) via a solvent extraction technique. Micelle loading greatly improved the solubility of ridaforolimus by approximately 40 times from 200 μg/mL to 8.9 mg/mL. The diameters of the drug-loaded micelles were 33 ± 15 nm indicating they are of appropriate size to accumulate within the tumor site via the enhanced permeability and retention (EPR) effect. The DSPE-PEG2000 micelle formulation was dosed intravenously to rats at 10 mg/kg and compared to a control of ridaforolimus in ethanol/PEG 400. The micelle significantly increased the half-life of ridaforolimus by 170% and decreased the clearance by 58%, which is consistent with improved retention of the drug in the plasma by the micelle formulation.
1A Processor: Control System (1977)

This article contains a description of the central control, input/output subsystem, processor-peripheral interface, master control console, and the interunit communication bus system. The units are discussed in a manner which highlights comparison of features with No. 1 ess and stresses those features which are most important in meeting the stringent reliability objectives.
Application of two-dimensional cellular automaton lattice-gas models to the simulation of hydrodynamics (1990)

II YDRODYNAMIC equations are notoriously difficult to solve, both analytically and computationally, therefore less complicated methods, drawing on the power ofcellular automata and lattice-gases are introduced.Their combined ability to capture the fundamental properties of fluid dynamics in an inherently simple manner is discussed.The FHP7 cellular automaton lattice-gas model of Frisch, Hasslacher and Pomeau, which will form the basis for the subsequent simulations, is described in detail, with a more general covering of the associated models. List of Tables1.1 Particle configuration classifications .........
Electronic Medical Record‐Integrated Pharmacogenomics and Related Clinical Decision Support Concepts (2017)

Advances in pharmacogenomics (PGx) have the potential to transform healthcare by allowing precision medicine to become a reality. However, PGx knowledge is new, complex, and evolving, and relying on the cognition of clinicians alone is insufficient for clinical implementation. Integrating clinical decision support (CDS) tools in the electronic health record (EHR) is critical for translating PGx into clinical practice. Herein, we review current strategies to implement PGx using EHR-CDS functionalities.
Microstructuring with femtosecond laser inside silica glasses (2003)

Summary form only given. Modification of the refractive index of transparent materials by UV laser radiation is widely used for writing waveguides and Bragg gratings in different type of glasses. Extending controlled writing to the inside of transparent materials is important since it opens a wide range of applications for integrated optical elements. In this work we report on direct writing of diffraction gratings and other optical elements inside different silica glasses with a train of femtosecond laser pulses at high repetition rate and study the influence of laser repetition rate and the number of pulses per focused area.
Methods for SBS threshold reduction (1994)

We have investigated methods for reducing the threshold for stimulated Brillouin scattering (SBS) using a frequency-narrowed Cr,Tm,Ho:YAG laser operating at 2.12 micrometers. The SBS medium was carbon disulfide. Single-focus SBS and threshold reduction by using two foci, a loop, and a ring have been demonstrated. Theories for the loop-SBS and the ring-SBS geometries have been developed and compared with experimental results. Backscatter power, energy, and conjugate fidelity data are presented.
P1I-1 A Novel Magnetic Field Sensor Using Piezoelectric Vibrations (2006)

This report presents novel configurations for magnetic field sensing using piezoelectric vibrators, which is based on a simple electromagnetic physics and requires no semiconductor materials. First, we present the principle and a basic configuration of the sensor as well as theoretical estimations of the sensitivity. Second, one of the basic configurations using longitudinal vibration for the proposed principle is prototyped, and its characteristics are evaluated experimentally. As an alternative configuration, a disk vibrator is tested. Other configurations are also suggested
Speech enhancement for bandlimited speech (2002)

Throughout the history of telecommunication, speech has rarely been transmitted with its full analog bandwidth (0 to 8 kHz or more) due to limitations in channel bandwidth. This impaired legacy continues with tactical voice communication. The passband of a voice terminal is typically 0 to 4 kHz. Hence, high-frequency speech components (4 to 8 kHz) are removed prior to transmission. As a result, speech intelligibility suffers, particularly for low-data-rate vocoders. In this paper, we describe our speech-processing technique, which permits some of the upperband speech components to be translated into the passband of the vocoder. According to our test results, speech intelligibility is improved by as much as three to four points even for the Department of Defense-standard mixed excitation linear predictor (MELP) 2.4 kb/s vocoder. Note that speech intelligibility is improved without expanding the transmission bandwidth or compromising interoperability with others.
Theoretical foundations of dynamical Monte Carlo simulations (1991)

Monte Carlo methods are utilized as computational tools in many areas of chemical physics. In this paper, we present the theoretical basis for a dynamical Monte Carlo method in terms of the theory of Poisson processes. We show that if: (1) a ‘‘dynamical hierarchy’’ of transition probabilities is created which also satisfy the detailed-balance criterion; (2) time increments upon successful events are calculated appropriately; and (3) the effective independence of various events comprising the system can be achieved, then Monte Carlo methods may be utilized to simulate the Poisson process and both static and dynamic properties of model Hamiltonian systems may be obtained and interpreted consistently.
Identification of inhibitors of nitric oxide synthase that do not interact with the endothelial cell<scp>l</scp>‐arginine transporter (1992)

The effects of inhibitors of nitric oxide (NO) synthase and other cationic amino acids on unidirectional L-arginine transport were studied in porcine aortic endothelial cells cultured in microwell plates or perfused in microcarrier columns. L-Homoarginine, L-lysine and L-ornithine inhibited transport of L-arginine. The NO synthase inhibitors NG-monomethyl-L-arginine and NG-iminoethyl-L-ornithine also reduced L-arginine uptake, whereas NG-nitro-L-arginine and its methyl-ester had no inhibitory effect. The ability to modulate selectively endothelial cell L-arginine transport or NO synthase activity will allow further characterization of the arginine transporter and its role in regulating NO biosynthesis.
Enhancement of human monocyte function against <i>Candida albicans</i> by the colony-stimulating factors (CSF): IL-3, granulocyte-macrophage-CSF, and macrophage-CSF. (1989)

The effect of IL-3, granulocyte-macrophage (GM)-CSF and macrophage (M)-CSF on Candida albicans growth inhibition by human peripheral blood monocytes was investigated. By using a radiolabel microassay developed in our laboratory that makes use of the incorporation of [3H]glucose into residual C. albicans, we demonstrated that rGM-CSF and rIL-3 effectively enhanced human monocyte-mediated anticandidal activity. Incubation for 24 h with either GM-CSF or IL-3 significantly enhanced monocyte antifungal responses down to 0.01 U/ml. M-CSF, at higher concentrations of 10 U/ml, could also enhance monocyte function but to a smaller degree. None of the CSF interfered directly with fungal growth, even up to 1000 U/ml. Because IFN-gamma is also a known monocyte activator, its effect on monocytes was also assessed. Monocytes were first cultured in medium for several days and then further incubated with each of the cytokines. Monocytes aged in medium were found to lose their spontaneous anticandidal activity. Such aged monocytes did not develop anticandidal activity in response to IFN-gamma but did in response to GM-CSF or IL-3. To further elucidate this difference, fresh monocytes were continuously cultured with or without cytokines for 1 to 5 days before assessing their anticandidal activity. Monocytes cultured in IFN-gamma progressively lost their activity by 2 days but monocytes in GM-CSF or IL-3 maintained their high level of anticandidal activity throughout the whole length of culture. Therefore, GM-CSF and IL-3 not only enhanced fresh monocyte anticandidal activity, but maintained monocyte function for a longer period. These results suggest that GM-CSF and IL-3 may act on monocytes via a different pathway than does IFN-gamma.