gebhart/patrepeval huggingface dataset:
Mitochondria and DNA Targeting of 5,10,15,20-Tetrakis(7-sulfonatobenzo[<i>b</i>]thiophene) Porphyrin-Induced Photodynamic Therapy via Intrinsic and Extrinsic Apoptotic Cell Death (2015)

Photodynamic therapy (PDT) selectively targets subcellular organelles and promises an excellent therapeutic strategy for cancer treatment. Here, we report the synthesis of a new water-soluble photosensitizer, 5,10,15,20-tetrakis (7-sulfonatobenzo[b]thiophene) porphyrin (SBTP). Rational design of the porphyrinic molecule containing benzo[b]thiophene moiety at the meso-position led to selective accumulation in both mitochondria and nucleus of MCF-7 cells. This multitarget ability of SBTP can cause damage to mitochondria as well as DNA simultaneously. FACS analysis showed rapid cellular uptake of SBTP. High-content cell-based assay was executed to concurrently monitor increase of cytosolic Ca(2+) levels, mitochondrial permeability transition (MPT), and caspase-3/7/8 activation in MCF-7 cells under the pathological condition caused by PDT action of SBTP. The study of cell death dynamics showed that PDT action of SBTP caused an increase in the MPT followed by an increase in cytosolic Ca(2+) level. The localization of SBTP in the mitochondria activated the intrinsic apoptotic pathway. Additionally, localization of SBTP in the nucleus led to DNA damage in MCF-7 cells. The DNA fragmentation that occurred by PDT action of SBTP was thought to be responsible for extrinsic apoptosis of MCF-7 cells. SBTP demonstrated effective PDT activity of 5 μM IC50 value to MCF-7 cells by bitargeting mitochondria and DNA.
Delay-Based Network Utility Maximization (2010)

It is well known that max-weight policies based on a queue backlog index can be used to stabilize stochastic networks, and that similar stability results hold if a delay index is used. Using Lyapunov Optimization, we extend this analysis to design a utility maximizing algorithm that uses explicit delay information from the head-of-line packet at each user. The resulting policy is shown to ensure deterministic worst-case delay guarantees, and to yield a throughput-utility that differs from the optimally fair value by an amount that is inversely proportional to the delay guarantee. Our results hold for a general class of 1-hop networks, including packet switches and multi-user wireless systems with time varying reliability.
Recursive Total Least-Squares Algorithm Based on Inverse Power Method and Dichotomous Coordinate-Descent Iterations (2015)

We develop a recursive total least-squares (RTLS) algorithm for errors-in-variables system identification utilizing the inverse power method and the dichotomous coordinate-descent (DCD) iterations. The proposed algorithm, called DCD-RTLS, outperforms the previously proposed RTLS algorithms, which are based on the line-search method, with reduced computational complexity. We perform a comprehensive analysis of the DCD-RTLS algorithm and show that it is asymptotically unbiased as well as being stable in the mean. We also find a lower bound for the forgetting factor that ensures mean-square stability of the algorithm and calculate the theoretical steady-state mean-square deviation (MSD). We verify the effectiveness of the proposed algorithm and the accuracy of the predicted steady-state MSD via simulations.
CFP-based shear wave velocity model building using converted waves (2019)

This paper reveals a sequence of procedures for estimating shear seismic wave velocities and the subsequent generation of seismic images using converted waves. The PS-wave images produced by this workflow are fully correlated with the PP-wave images where both can be produced in the same coordinate system without any squeezing. The correlation between PP and PS images provides cross-validation and estimation of underground rock and fluid properties. In this workflow, there are two essential new supporting techniques. The first one is decoupling the S-wave information by generating CFP (common focus point) gathers via applying the P-wave velocity on the PS seismic data, and the second is to pick an optimal Vp/Vs ratio on the newly proposed Vp/Vs ratio semblance spectrum. The S-wave velocity, computed from the picked optimal ratio, can be used for generating not only the PS-wave images, but also for ensuring the PS image well correlated to P-wave image. Presentation Date: Monday, September 16, 2019 Session Start Time: 1:50 PM Presentation Time: 2:40 PM Location: 225C Presentation Type: Oral
Gestures Maintain Spatial Imagery (2001)

Recent theories suggest alternatives to the commonly held belief that the sole role of gestures is to communicate meaning directly to listeners. Evidence suggests that gestures may serve a cognitive function for speakers, possibly acting as lexical primes. We observed that participants gestured more often when describing a picture from memory than when the picture was present and that gestures were not influenced by manipulating eye contact of a listener. We argue that spatial imagery serves a short-term memory function during lexical search and that gestures may help maintain spatial images. When spatial imagery is not necessary, as in conditions of direct visual stimulation, reliance on gestures is reduced or eliminated.
Clonal B cells in patients with hepatitis C virus–associated mixed cryoglobulinemia contain an expanded anergic CD21low B-cell subset (2011)

Hepatitis C virus (HCV) is associated with the B-cell lymphoproliferative disorders mixed cryoglobulinemia (MC) and non-Hodgkin lymphoma. We have previously reported that HCV(+)MC(+) patients have clonal expansions of hypermutated, rheumatoid factor-bearing marginal zone-like IgM(+)CD27(+) peripheral B cells using the V(H)1-69 gene. Here we coupled transcriptional profiling with immunophenotypic and functional studies to ascertain these cells' role in MC pathogenesis. Despite their fundamental role in MC disease, these B cells have overall transcriptional features of anergy and apoptosis instead of neoplastic transformation. Highly up-regulated genes include SOX5, CD11C, galectin-1, and FGR, similar to a previously described FCRL4(+) memory B-cell subset and to an "exhausted," anergic CD21(low) memory B-cell subset in HIV(+) patients. Moreover, HCV(+)MC(+) patients' clonal peripheral B cells are enriched with CD21(low), CD11c(+), FCRL4(high), IL-4R(low) memory B cells. In contrast to the functional, rheumatoid factor-secreting CD27(+)CD21(high) subset, the CD27(+)CD21(low) subpopulation exhibits decreased calcium mobilization and does not efficiently differentiate into rheumatoid factor-secreting plasmablasts, suggesting that a large proportion of HCV(+)MC(+) patients' clonally expanded peripheral B cells is prone to anergy and/or apoptosis. Down-regulation of multiple activation pathways may represent a homeostatic mechanism attenuating otherwise uncontrolled stimulation of circulating HCV-containing immune complexes.
The Antimicrobial Antiproteinase Elafin Binds to Lipopolysaccharide and Modulates Macrophage Responses (2005)

Lipopolysaccharides (LPS) of the outer membrane of Gram-negative bacteria represent a primary target for innate immune responses. We demonstrate here that the antimicrobial/anti-neutrophil elastase full-length elafin (FL-EL) is able to bind both smooth and rough forms of LPS. The N-terminus was shown to bind both forms of LPS more avidly. We demonstrate that the lipid A core-binding proteins polymyxin B (PB) and LPS-binding protein (LBP) compete with elafin for binding, and that LBP is able to displace prebound elafin from LPS. When PB, FL-EL, N-EL, and C-EL were pre-incubated with LPS before addition to immobilized LBP, PB was the most potent inhibitor of LPS transfer to LBP. These data prompted us to examine the biological consequences of elafin binding to LPS, using tumor necrosis factor (TNF)-alpha release by murine macrophages. In serum-containing conditions, N-EL had no effect, whereas both C-EL and FL-EL inhibited TNF-alpha production. In serum-free conditions, however, all moieties had a stimulatory activity on TNF-alpha release, with C-EL being the most potent at the highest concentration. The differential biological activity of elafin in different conditions suggests a role for this molecule in either LPS detoxification or activation of innate immune responses, depending on the external cellular environment.
An Organ-Specific Differentiation Gene, <i>pha-1</i> , from <i>Caenorhabditis elegans</i> (1990)

Embryonic lethal mutations in the nematode Caenorhabditis elegans were generated and screened for phenotypes that suggest regulatory functions in order to identify genes involved in the control ofearly development. In embryos homozygous for mutations in one such gene, pha-1, the pharynx fails to undergo late differentiation and morphogenesis. Early pharynx development is not affected; thus, pha-l controls the latter stages of this developmental process. All markers specific for differentiation in various pharyngeal cell types tested are affected, suggesting that pha-1 acts in an organ-specific, rather than cell type-specific, manner. The temperature-sensitive phases of both temperature sensitive mutations indicate that pha-l function is required solely during midembryogenesis, shortly before the onset of morphogenesis.
Antibodies reactive with the mouse mammary tumor virus in sera of breast cancer patients (1980)

IgG binding to purified mouse mammary tumor virus (MuMTV) was quantitated by an enzyme-linked immunoassay (ELISA) using sera from patients with breast cancer or benign breast disease, or from healthy age-matched controls. Significantly greater binding (p<0.01) was found in breast-cancer-derived sera than in the other categories. In addition to IgG reactivity, three breast cancer sera also possessed IgA and IgM reactive with MuMTV by the ELISA assay. Only IgG was reactive in the majority of sera while two sera possessed MuMTV binding activity only in the IgM fraction. Both IgG binding and virolysis of MuMTV were greatly reduced by preincubation of sera with MuMTV. The specificity for MuMTV was further explored with IgG of serum from one breast cancer patient. Human antibody reactive with MuMTV was progressively diminished by preincubating the human serum with increasing concentrations of MuMTV but not by incubation with the type-C AKR murine leukemia virus. Preincubation of MuMTV with a breast cancer serum partially blocked the reactivity of gp52 antiserum with the virus. The results suggest that an antigen related to an MuMTV envelope component is expressed in breast cancer.
Effect of Cu Loading Methods on Electrochemical Characteristics of Cu-activated Carbon Composite Electrodes (2003)

Cu-activated carbon composite electrodes were prepared by the impregnation method and the mixing method. The porous structures and electrochemical double-layer capacitance of activated carbons loaded with metal Cu were investigated by using nitrogen gas adsorption and constant current cycling (CCC) methods. The results show that activated carbon loaded with metal Cu can improve the discharge capacitance of the electrodes, however, the different loading methods will lead to the varied influences on the cycle characterization of the electrodes. SEM, TEM and XRD technologies show that the stable electrochemical characterization obtained from the activated carbon mixed metal Cu electrode mainly depends on the metal particles dispersed homogeneously in all parts of the activated carbon.
A Vivência da Amamentação em “Mães de Primeira Viagem” (2006)

Breast-feeding exceeds the boundaries of biology, inserting itself also in the social and psychological scopes. This study, taken place in the Obstetric Clinic of the ICHC-FMUSP, used the qualitative methodology to interview in depth ten first-time mothers, in order to understand the psychological aspects involved in breast-feeding, during the baby’s first week of life, and how these mothers dealt with the guidance given by the health team. Individual interviews and qualitative analysis of the data has been carried through. Breast-feeding appeared as an ambivalent experience, with feelings of power and completeness, femininity, but also appeared as a painful experience. Half of the interviewed mothers received guidance from the health team, and felt it as an emotional support and security. Thus, breast-feeding, when it is not imposed, may favor the meeting of mother and child, as well as the contact of the mother with herself. The health team can act as a facilitator, using technical knowledge, but always keeping themselves opened to listening to these women.
Inhibition of Human Sirtuins by in Situ Generation of an Acetylated Lysine−ADP−Ribose Conjugate (2009)

A new type of small-molecular sirtuin inhibitor was designed on the basis of the proposed catalytic mechanism for deacetylation of acetylated lysine substrates by sirtuins. Among the compounds thus designed and synthesized, we found that 2k, which contains an ethoxycarbonyl group at the alpha position to the acetamide of acetylated lysine substrate analogue 1, showed potent inhibitory activity in an in vitro assay using recombinant SIRT1, with high selectivity over SIRT2 and SIRT3. Mechanistic study by means of kinetic analysis, mass spectroscopy, and computation indicated that the enol form of compound 2k nucleophilically attacks NAD(+) in the active site of SIRTs to afford the stable compound 2k-ADP-ribose conjugate 5, leading to inhibition of the enzyme activity. Compound 2k also caused a dose-dependent increase of p53 acetylation in human colon cancer HCT116 cells, indicating inhibition of SIRT1 in the cells. These results have implications for the development of selective sirtuin inhibitors by means of mechanism-based drug design.
Differentiation plasticity of hematopoietic cells (2002)

During the past few years an avalanche of papers describing the conversion of transplanted bone marrow cells into skeletal muscle, heart, liver, brain, and various epithelial tissues suggested a surprising degree of hematopoietic stem cell plasticity. For the clinician they raised the expectation
Nonvolatile multilevel conductance and memory effects in organic thin films (2005)

Organic thin-film structures, including organic light-emitting diodes, are demonstrated to contain multiple nonvolatile conductance states at low-read voltages. Retention time of states is more than several weeks, and more than 20 000 write-read-rewrite-read cycles have been performed with minimal degradation. The electrical characteristics of these devices are consistent with metal diffusion or filament phenomena found in metal-insulator-metal structures, suggesting a possible mechanism by which the states are stored.
A rapid method for in situ hybridization for viral DNA in brain biopsies from patients with AIDS (1989)

Brain biopsy is often necessary in the diagnosis of neurological complications found in AIDS patients. We describe here a rapid method of tissue preparation and in situ DNA hybridization for detecting JC virus DNA in frozen brain biopsy sections which allows the diagnosis of progressive multifocal leukoencephalopathy to be established on the day of surgery. Once the diagnosis is established, therapeutic and management decisions can be made more easily. The commercial availability of biotinylated probes for several of the DNA viruses most frequently encountered in brain infections of AIDS patients will provide wide application of these techniques to patient management.
Implementation of a single-phase Multilevel Inverterwith Battery balancing (2014)

In this paper, a single-phase multilevel inverter with battery balancing is proposedThe voltage source inverters produce a voltage with levels either 0 or ±V dc they are known as two level inverters.To obtain a quality output voltage or a current waveform with a minimum amount of ripple content, they require high switching frequency along with various pulse width modulation (PWM) strategies.As the number of voltage levels increases, the harmonic content of output voltage waveform decreases significantly.Battery systems are affected by many factors, a key one being the cells unbalancing. Without the balancing system, the individual cell voltages will differ over time, battery pack capacity will decrease quickly. That will result in the failure of the total battery system. Thus cellbalancing acts an important role on the battery life preserving. Different cell balancing methodologies have been proposed for battery pack. In this paper, the general structure of multi-level converter is to synthesize a near sinusoidal voltage from several levels of dc voltages.The operational principle of the proposed system is first described, and then, the design equation is derived. Simulation results are obtained for single phase 9-level inverter with battery balancing with MATLAB/Simulink. Keywords— Inverter, PWM, Battery pack capacity ,Harmonic
Multan Electric Power Company Limited (MEPCO 6th STG and ELR Project) (2006)

The development objective of the Electricity Distribution and Transmission Improvement Project for Pakistan is to help increase the efficiency, reliability, and quality of electricity supply by supporting reductions in overall technical and commercial losses, increased availability of electricity, and improved voltage profile. This resettlement plan (RP) evaluated resettlement risks associated with the project and recommends measures to mitigation them. In this regard mitigation measures include : all project affected peoples (PAPs) are equally eligible for compensation and rehabilitation assistance, irrespective of land ownership status, to ensure that those affected by the project will be at least as well off, if not better off than they would have been without the project; absence of title will not be an impediment for PAPs to receive compensation and rehabilitation assistance; compensation packages will reflect replacement costs for all losses, such as land, crops, structures, trees, businesses, income, etc.; the project will pay cash compensation for the replacement cost of religious sites, alternatively the project will construct the religious site, in consultation with the affected community; the project will also pay cash for the relocation of graves; and finally, PAPs will be systematically informed and consulted about design and implementation stages of the project, and the RP will be disclosed and made available to the affected persons and communities.
No. 1 ESS Bus System (1964)

Communication between the various units within no. 1 ESS demands a complex network of transmission lines. The requirements are radically different from previous offices became a large amount of information must be transmitted at high speed, in digital form, between a multiplicity of locations with a high degree of reliability. A description is given of objectives and of organization and operational modes for the network. The problems involved are discussed together with the hardware by which the objectives were successfully realized.
Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis (1994)

During a previous study of the opsonic requirements for neutrophil (polymorphonuclear leukocyte [PMN])-mediated killing of enterococci, we identified two strains of Enterococcus faecium (TX0015 and TX0016) that were resistant to PMN-mediated killing. To better define the mechanism of this resistance, we examined phagocytosis with a fluorescence assay and found that TX0016 was completely resistant to phagocytosis by PMNs; this finding was confirmed by electron microscopy. Examination of multiple strains of enterococci revealed that all 20 strains of Enterococcus faecalis tested were readily phagocytosed (mean, 18 intracellular organisms per PMN; range, 7 to 28). In contrast, only 13 (50%) of 26 strains of E. faecium tested were susceptible to phagocytosis (> or = 7 organisms per PMN); the other 13 strains showed < or = 3 organisms per PMN. Enterococcus casseliflavus ATCC 25788 and one strain of Enterococcus hirae were also resistant to phagocytosis, while two strains of Enterococcus durans, Enterococcus mundtii ATCC 43186, and one strain each of Enterococcus raffinosus and Enterococcus solitarius were readily phagocytosed. Exposure of E. faecium TX0016 to sodium periodate, but not to the protease trypsin or pronase or to phospholipase C, eliminated resistance to phagocytosis. Sialic acid, a common periodate-sensitive structure used by microorganisms to resist opsonization, could not be demonstrated in E. faecium TX0016 by the thiobarbituric acid method, nor was phagocytosis of TX0016 altered by neuraminidase treatment. This study suggests that there is a difference in susceptibility to phagocytosis by PMNs between different species of enterococci and that a carbohydrate-containing moiety which is not sialic acid may be involved in the resistance of E. faecium TX0016 to phagocytosis.
Mutineries et grèves de femmes (2000)

En France, le début de l’année 1917 est marqué par une renaissance de l’espoir. Au mois de décembre 1916 on avait perçu l’offre de paix allemande comme un signe de faiblesse, et la rupture des relations diplomatiques entre l’Allemagne et les États-Unis comme un signe positif. Les espérances se focalisent sur la personne de Robert Nivelle, nouveau commandant en chef des armées. Il prépare une offensive franco-britannique sur le front occidental, promet une percée fulgurante, suivie d’une victo...
Dual-polarized linear series-fed microstrip arrays with very low losses and cross polarization (2004)

The design of a microstrip series-fed linear traveling-wave array is described in this paper. The array is composed of two symmetric subarrays formed by cascading an equal number of four-port aperture-coupled cross-patch elements and terminated on a two-port radiating matched load. Dual-linear and circular polarization can be achieved by appropriately exciting the array and by virtue of the symmetric arrangement of the antenna high polarization purity is obtained. A proof-of-concept linear array is developed and the corresponding numerical results performed through a full-wave approach based on the method of moments are presented.
Multi-walled carbon nanotubes affect drug transport across cell membrane in rat astrocytes (2010)

The impact of carbon nanotubes on the cell membrane is an aspect of particular importance and interest in the study of carbon nanotubes' interactions with living systems. One of the many functions of the cell membrane is to execute substance transport into and out of the cell. We investigated the influence of multi-walled carbon nanotubes (MWCNTs) on the transport of several compounds across in the cell membrane of rat astrocytes using flow cytometry. These compounds are fluorescein diacetate, carboxyfluorescein diacetate, rhodamine 123 and doxorubicin, which are prosubstrate/substrates of multidrug transporter proteins. Results showed that MWCNTs significantly inhibited cellular uptake of doxorubicin but not the other drugs and the mode of loading made a significant difference in doxorubicin uptake. Retention of fluorescein, carboxyfluorescein and rhodamine 123 was remarkably higher in MWCNT-exposed cells after an efflux period. A kinetics study also demonstrated slower efflux of intracellular fluorescein and rhodamine 123. Data presented in this paper suggest that MWCNTs could affect drug transport across cell membranes. The implications of the findings are discussed.