gebhart/patrepeval huggingface dataset:
Single Domain Intracellular Antibodies from Diverse Libraries (2010)

Interfering intracellular antibodies are valuable for biological studies as drug surrogates and as potential macromolecular drugs per se. Their application is still limited because of the difficulty of acquisition of functional intracellular antibodies. We describe the use of the new intracellular antibody capture procedure (IAC(3)) to facilitate direct isolation of functional single domain antibody fragments using four independent target molecules (LMO2, TP53, CRAF1, and Hoxa9) from a set of diverse libraries. Initially, these have variability in only one of the three antigen-binding CDR regions of VH or VL and first round single domains are affinity matured by iterative randomization of the two other CDRs and reselection. We highlight the approach using a single domain binding to LMO2 protein. Our results show that interfering with LMO2 protein function demonstrates a role specifically in erythroid differentiation, confirm a necessary and sufficient function for LMO2 as a cancer therapy target in T-cell neoplasia and allowed for the first time production of soluble recombinant LMO2 protein by co-expression with intracellular domain antibodies. Co-crystallization of LMO2 and the anti-LMO2 VH protein was successful. These results demonstrate that this third generation IAC(3) offers a robust toolbox for various biomedical applications and consolidates functional features of the LMO2 protein complex, which includes the importance of Lmo2-Ldb1 protein interaction.
Weight Reduction Effect of Puerh Tea in Male Patients with Metabolic Syndrome (2014)

Puerh tea has been proposed to promote weight loss and favorably modify glucose, insulin and blood lipids. This study tested the effect of daily Puerh tea consumption for 3 months on weight and body mass index (BMI), and select metabolic parameters. The effect of daily Puerh tea intake on weight, BMI and changes in glucose, HbA1c and lipids was evaluated in patients with metabolic syndrome. The patients (N = 70) were randomized into two groups: those taking Puerh tea extract capsule (333 mg Puerh tea extract) three times a day and those taking a placebo tea for 3 months. There was a decrease in body weight of 1.3 kg in the Puerh tea group (p = 0.077) versus 0.23 kg in the placebo arm (p = 0.186). There was also a slight decrease in BMI 0.47 kg/m(2) in the Puerh tea group (p = 0.076) versus 0.09 kg/m(2) in the placebo arm (p = 0.185), suggesting a trend of weight change, but without statistical significance. Subgroup analysis of the male patients demonstrated statistically significant improvements in body weight reduction (p = 0.004) and BMI (p = 0.004). However, the change in other metabolic parameters (cholesterol or triglyceride) or HbA1c was not statistically significant. Intake of Puerh tea for 3 months was associated with a slight reduction in body weight and BMI, especially in the male patients. Therefore, daily Puerh tea consumption may be an alternative choice to modify body weight.
Neutral, panchromatic Ru(ii) terpyridine sensitizers bearing pyridine pyrazolate chelates with superior DSSC performance (2009)

A new series of neutral, panchromatic Ru(II) terpyridine sensitizers (PRT1-PRT4) exhibit much higher molar extinction coefficients at 400-550 nm and superior DSSC performance in terms of conversion efficiency (eta = 10.05 for PRT4) and stability.
Expression of TGF‐β isoforms and their receptors during mineralized nodule formation by rat periodontal ligament cells <i>in vitro</i> (1999)

Transforming growth factor-betas (TGF-beta s) and bone morphogenetic proteins (BMPs), members of a TGF-beta superfamily, are known to play an important role in osteogenic cell differentiation and consequently bone formation. We have reported previously that periodontal ligament (PDL) cells differentiate and form mineralized nodules when cultured in the presence of dexamethasone (Dex), beta-glycerophosphate (GP) and ascorbic acid (AA). To understand the roles of TGF-beta isoforms (TGF-beta 1, 2 and 3) and TGF-beta type I receptors (activin receptor-like kinase (ALK)-2, -3, -5 and -6) in PDL cell differentiation, their expression was investigated using Northern blot analysis. Rat PDL cells, derived from coagulum in the tooth socket, were cultured in the presence of Dex (5 microM), GP (10 mM) and AA (50 micrograms/ml) for up to 21 d. Total RNA was isolated from PDL cells after 0, 7, 14 and 21 d and used for northern blot analysis of mRNAs for matrix proteins, TGF-beta isoforms and their receptors using 32P-labeled cDNAs as probes. Four stages showing distinct morphological characteristics and matrix expression during development of mineralized nodules were identified. Type I collagen (Col I) and SPARC (secreted protein, acidic and rich in cysteine) mRNAs were expressed at the confluent stage, but decreased during the mineralization stage. Osteopontin (OPN) and alkaline phosphatase (ALP) transcripts were initially observed at multilayer stage, while bone sialoprotein (BSP) and osteocalcin (OC) at the nodule stage and all 4 were expressed thereafter. TGF-beta 1 mRNA expression increased with the progression of PDL cell differentiation, while a relatively high level of TGF-beta 3 transcript decreased slightly during their differentiation. TGF-beta 2 mRNA was not expressed. The expression of TGF beta-RI mRNA decreased, whereas that of TGF beta-RIII increased dramatically with PDL cell differentiation. TGF beta-RII gene activities remained high throughout all stages. ALK-2, ALK-3 and ALK-6 mRNA expression increased with the progression of PDL cell differentiation, suggesting that these receptors may play important roles in Dex-induced PDL cell differentiation and mineralized nodule formation.
SUBJECTIVE SYMPTOMS (1925)

Optometry and Vision Science is the most authoritative source for current developments in optometry, physiological optics, and vision science. This frequently cited monthly scientific journal has served primary eye care practitioners for more than 75 years, promoting vital interdisciplinary exchange among optometrists and vision scientists worldwide. Official Journal of the American Academy of Optometry.
The Immunogenicity of Modified Lipoproteins (2005)

The immunogenicity of modified low-density lipoprotein (mLDL) has been demonstrated both in laboratory animals and humans. Circulating human mLDL antibodies, purified by affinity chromatography, are predominantly of the IgG isotype, subclasses 1 and 3. The purified antibodies react with malondialdehyde-lysine and carboxymethyl-lysine epitopes, but also recognize minimally modified forms of LDL that do not contain significant amounts of those two epitopes. The quantitative assays of mLDL and mLDL antibodies in serum samples by enzymoimmunoassay (EIA) are unreliable owing to the interference of preformed circulating immune complexes (CICs). Isolation of CICs by precipitation with low concentrations of polyethylene glycol followed by analysis of antigens and antibodies contained in the precipitates is a technically complex approach, but one that yields valuable data. With this approach we have confirmed that the IgG antibodies involved in IC formation belong to the proinflammatory IgG1 and IgG3 isotypes, have a higher avidity than those that remain unbound in the supernatant after CIC precipitation, and are of higher avidity in diabetic patients with macroalbuminuria than in those with normal albuminuria. We have also developed capture assays for different forms of mLDL. These assays have shown a significant enrichment in mLDL of the precipitated ICs. The enrichment is also more pronounced in the CICs obtained from diabetic patients with macroalbuminuria. In conclusion, isolation and characterization of LDL-ICs appears to yield information of significant value that is not derived from other approaches to measure LDL modifications and their corresponding antibodies in humans.
No evidence for human papillomavirus infection in focal cortical dysplasia<scp>II</scp>b (2014)

Our data did not confirm previously reported evidence for HPV16 detection in FCDIIb.
Brain Activity Mapping in <i>Mecp2</i> Mutant Mice Reveals Functional Deficits in Forebrain Circuits, Including Key Nodes in the Default Mode Network, that are Reversed with Ketamine Treatment (2012)

Excitatory-inhibitory imbalance has been identified within specific brain microcircuits in models of Rett syndrome (RTT) and other autism spectrum disorders (ASDs). However, macrocircuit dysfunction across the RTT brain as a whole has not been defined. To approach this issue, we mapped expression of the activity-dependent, immediate-early gene product Fos in the brains of wild-type (Wt) and methyl-CpG-binding protein 2 (Mecp2)-null (Null) mice, a model of RTT, before and after the appearance of overt symptoms (3 and 6 weeks of age, respectively). At 6 weeks, Null mice exhibit significantly less Fos labeling than Wt in limbic cortices and subcortical structures, including key nodes in the default mode network. In contrast, Null mice exhibit significantly more Fos labeling than Wt in the hindbrain, most notably in cardiorespiratory regions of the nucleus tractus solitarius (nTS). Using nTS as a model, whole-cell recordings demonstrated that increased Fos expression in Nulls at 6 weeks of age is associated with synaptic hyperexcitability, including increased frequency of spontaneous and miniature EPSCs and increased amplitude of evoked EPSCs in Nulls. No such effect of genotype on Fos or synaptic function was seen at 3 weeks. In the mutant forebrain, reduced Fos expression, as well as abnormal sensorimotor function, were reversed by the NMDA receptor antagonist ketamine. In light of recent findings that the default mode network is hypoactive in autism, our data raise the possibility that hypofunction within this meta-circuit is a shared feature of RTT and other ASDs and is reversible.
Generative adversarial networks for single image super resolution in microscopy images (2018)

Image Super resolution is a widely-studied problem in computer vision, where the objective is to convert a lowresolution image to a high resolution image. Conventional methods for achieving super-resolution such as image priors, interpolation, sparse coding require a lot of pre/post processing and optimization. Recently, deep learning methods such as convolutional neural networks and generative adversarial networks are being used to perform super-resolution with results competitive to the state of the art but none of them have been used on microscopy images. In this thesis, a generative adversarial network, mSRGAN, is proposed for super resolution with a perceptual loss function consisting of a adversarial loss, mean squared error and content loss. The objective of our implementation is to learn an end to end mapping between the low / high resolution images and optimize the upscaled image for quantitative metrics as well as perceptual quality. We then compare our results with the current state of the art methods in super resolution, conduct a proof of concept segmentation study to show that super resolved images can be used as a effective pre processing step before segmentation and validate the findings statistically.
The jennings case (1912)

Journal Article The jennings case Get access Sydney Herbert Sydney Herbert 1Carlton Lodge, Cheltenham Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume s11-V, Issue 114, 2 March 1912, Pages 175–176, https://doi.org/10.1093/nq/s11-V.114.175c Published: 02 March 1912
Polarizations on abelian varieties (2002)

Every isogeny class over an algebraically closed field contains a principally polarized abelian variety ([ 10 , corollary 1 to theorem 4 in section 23]). Howe ([ 3 ]; see also [ 4 ]) gave examples of isogeny classes of abelian varieties over finite fields with no principal polarizations (but not with the degrees of all the polarizations divisible by a given non-zero integer, as in Theorem 1·1 below). In [ 17 ] we obtained, for all odd primes [lscr ], isogeny classes of abelian varieties in positive characteristic, all of whose polarizations have degree divisible by [lscr ] 2 . We gave results in the more general context of invertible sheaves; see also Theorems 6·1 and 5·2 below. Our results gave the first examples for which all the polarizations of the abelian varieties in an isogeny class have degree divisible by a given prime. Inspired by our results in [ 17 ], Howe [ 5 ] recently obtained, for all odd primes [lscr ], examples of isogeny classes of abelian varieties over fields of arbitrary characteristic different from [lscr ] (including number fields), all of whose polarizations have degree divisible by [lscr ] 2 .
High selectivity SAW duplexer for W-CDMA Band VIII (2008)

Special requirements for Band VIII Wideband CDMA (W-CDMA) duplexers are the relatively large bandwidths of 3.9% for transmitting (TX) and 3.7% for receiving (RX) filters as well as the low duplex distance of 1.1%. Moreover, compared to systems at higher frequencies, for 1 GHz systems increased focus is put on miniaturization. In order to achieve good RX suppression within the TX band, the RX filter is comprised of one port resonators as well as a DMS track whereas the TX filter is a pure ladder type filter. The center frequencies of these building blocks show different dependencies with respect to metallization ratio and layer thickness. The consideration of this relationship within the design process was necessary to achieve a very low shift of the right skirt of the TX filter relative to the left skirt of the RX filter. Our duplexer is realized in a 3.0x2.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> package on LTCC ceramic and offers a suppression of the W-CDMA RX-signal of 50 dB within the TX band. Typical values for attenuation of the W-CDMA signal in the transmission band are 1.7 dB for the TX path and 2.0 dB for the RX path. The comparison between simulation and measurement shows the applicability of the design concept which is the most appropriate one for duplexers with small duplex distance and good RX suppression within the TX band.
Selling Information Through Consulting (2019)

We consider a monopoly information holder selling information to a budget-constrained decision maker, who may benefit from the seller's information. The decision maker has a utility function that depends on his action and an uncertain state of the world. The seller and the buyer each observe a private signal regarding the state of the world, which may be correlated with each other. The seller's goal is to sell her private information to the buyer and extract maximum possible revenue, subject to the buyer's budget constraints. We consider three different settings with increasing generality, i.e., the seller's signal and the buyer's signal can be independent, correlated, or follow a general distribution accessed through a black-box sampling oracle. For each setting, we design information selling mechanisms which are both optimal and simple in the sense that they can be naturally interpreted, have succinct representations, and can be efficiently computed. Notably, though the optimal mechanism exhibits slightly increasing complexity as the setting becomes more general, all our mechanisms share the same format of acting as a consultant who recommends the best action to the buyer but uses different and carefully designed payment rules for different settings. Each of our optimal mechanisms can be easily computed by solving a single polynomial-size linear program. This significantly simplifies exponential-size LPs solved by the Ellipsoid method in the previous work, which computes the optimal mechanisms in the same setting but without budget limit. Such simplification is enabled by our new characterizations of the optimal mechanism in the (more realistic) budget-constrained setting.
Capillary gas-liquid chromatographic/mass spectrometric measurement of plasma acetate content and (2-13C) acetate enrichment (1989)

In studies where (2-13C)acetate is employed as isotopic tracer in vivo, we have reported a selected ion monitoring gas-liquid chromatographic/mass spectrometric method which allows plasma tracer enrichment as well as plasma acetate content to be determined in the same 200-500 microliters sample through the use of methacrylic acid as the assay internal standard. For standard solutions in the range equivalent to plasma acetic acid concentrations of 10-200 microM, assay precision was +/- 4.3%. For plasma samples in the physiological range (approximately 20-300 microM acetate) assay precision averaged better than +/- 5%. The use of the method is illustrated by measuring acetate turnover in a young adult.
Combined genetic transformation and nutritional assay for identification of Moraxella nonliquefaciens (1987)

A combined genetic transformation and nutritional assay is described that permits definitive identification of clinically isolated strains of Moraxella nonliquefaciens. Crude DNA preparations of strains of various Moraxella species were used to transform nutritional mutants of a stably competent strain of M. nonliquefaciens for ability to grow on a defined medium (Mn-B). DNA samples from 24 independently isolated strains of M. nonliquefaciens all resulted in massive (4+) transformation of each of two mutant assay strains. DNA samples from strains of M. bovis and M. lacunata frequently gave weak (1+) transformation of one of the mutant assay strains (Mn64) but almost always failed to transform another assay strain (Mn136). DNA samples from eight other Moraxella species failed completely to transform either of the mutant assay strains. When streaked on the defined medium used for the transformation assay (Mn-B), 23 of the 24 strains of M. nonliquefaciens grew well, but all strains of M. bovis and M. lacunata failed to grow on this medium.
[Investigation of CD133 as putative marker of tumor-initiating cell in laryngeal carcinoma]. (2007)

CD133 is one of makers for cancer stem cell in human larynx tumors, Hep-2 cell line. Identification of it provides a powerful tool to investigate the tumorigenic process in the larynx and to develop therapies targeted to the tumor-initiating cell.
Real-time data-driven interactive rough sketch inking (2018)

We present an interactive approach for inking , which is the process of turning a pencil rough sketch into a clean line drawing. The approach, which we call the Smart Inker , consists of several "smart" tools that intuitively react to user input, while guided by the input rough sketch, to efficiently and naturally connect lines, erase shading, and fine-tune the line drawing output. Our approach is data-driven: the tools are based on fully convolutional networks, which we train to exploit both the user edits and inaccurate rough sketch to produce accurate line drawings, allowing high-performance interactive editing in real-time on a variety of challenging rough sketch images. For the training of the tools, we developed two key techniques: one is the creation of training data by simulation of vague and quick user edits; the other is a line normalization based on learning from vector data. These techniques, in combination with our sketch-specific data augmentation, allow us to train the tools on heterogeneous data without actual user interaction. We validate our approach with an in-depth user study, comparing it with professional illustration software, and show that our approach is able to reduce inking time by a factor of 1.8X, while improving the results of amateur users.
Increased phospholipase C activity after experimental brain injury (1982)

Phospholipase C activity was measured in 1000 X G centrifuged cellular fractions isolated from cerebral cortical homogenates obtained from either control cats or cats subjected to experimental fluid-percussion brain injury. Phospholipase C activity was determined directly by measuring the Ca++-dependent conversion of membrane-bound, labeled phosphatidate to diacylglycerol or indirectly by measuring the diacylglycerol-dependent (brain diacylglycerol content) formation of phosphatidylcholine in the presence of labeled cytidine diphosphate (CDP) choline. Phospholipase C activity determined by either method was about two time greater in cell fractions isolated from animals subjected to brain injury than in controls (p less than 0.01). The brain injury-induced rise in phospholipase C activity may be responsible, at least in part, for generating diacylglycerol that may be a source of free arachidonic acid that stimulates prostaglandin synthesis. These changes may account for the rise in brain prostaglandin levels that has been demonstrated earlier to occur after this type of brain injury.
Designing an Integrated Architecture for Network Content Security Gateways (2006)

Installing multiple network security products to detect and block viruses, spam, and other intrusions introduces substantial overhead in interprocess communications and kernel/user space interactions. Tightly integrating these functions in a single gateway can streamline the packet flows and dramatically increase Web and mail traffic throughput
MR identification of Chiari pathophysiology by using spatial and temporal CSF flow indices and implications for syringomyelia. (2003)

What key parameters should be used to evaluate symptomatic Chiari malformations? Conventional MR imaging is necessary to define the anatomic features of the brain and to describe the abnormalities of the posterior fossa and cerebellar tonsils. This is not always sufficient to show the extent or
Biological and mechanical characteristics of the interface between a new swelling anchor and bone (2000)

We recently evaluated the peak pullout loads for anchors made from our new copolymeric swelling-type material compared with anchors made of a nonswelling material. In vitro and in vivo peak pullout loads of these anchors were evaluated after different intervals of implantation in the lateral femoral condyles of New Zealand White rabbits. Scanning electron microscopy and energy dispersive x-ray analyses were additionally performed on selected retrieved samples after pullout to examine the characteristics of bone attachment to the implant. The mean peak pullout load was greater for the swelling anchors than for the nonswelling anchors after 48 hours in vitro (46.0 +/- 15.8 compared with 10.8 +/- 9.1 N, p = 0.0541). After 2 weeks in vivo, it was significantly greater for the swelling anchors than for the nonswelling controls (177.7 +/- 41.3 compared with 53.7 +/- 17.5 N, p = 0.0024). The peak pullout load was also greater for the swelling anchors after 8 weeks in vivo; however, this difference was less pronounced than at 2 weeks (101.8 +/- 35.0 compared with 58.9 +/- 9.7 N, p = 0.0508). Furthermore, the swelling implants tended to induce bone deposition at the bone-implant interface. Results from this investigation reveal that the new family of dynamic implants has potential for applications requiring fixation to cancellous or osteoporotic bone.
Evidence of homologous relationship between thermolysin and neutral protease A of Bacillus subtilis. (1975)

A comparison of the partial amino-acid sequence of neutral protease A from Bacillus subtilis with the structure of thermolysin (EC 3.4.24.4) from Bacillus thermoproteolyticus reveals that these two proteins are homologous. Of 171 residues placed in neutral protease (54% of the sequence), 83 residues (49%) occur in identical positions in thermolysin, and include nine of the 13 residues previously identified as components of the active site of thermolysin. This similarity provides support for the hypothesis that the two enzymes have similar three-dimensional structures and a common mechanism of action. Since these enzymes differ markedly in their resistance to heat inactivation, a comparison of their structures may eventually provide a chemical basis for explaining the differences in their thermal stability.