gebhart/patrepeval huggingface dataset:
Calmodulin Colocalizes with Connexins and Plays a Direct Role in Gap Junction Channel Gating (2001)

The direct calmodulin (CaM) role in chemical gating was tested with CaM mutants, expressed in oocytes, and CaM-connexin labeling methods. CaMCC, a CaM mutant with greater Ca-sensitivity obtained by replacing the N-terminal EF hand pair with a duplication of the C-terminal pair, drastically increased the chemical gating sensitivity of Cx32 channels and decreased their Vj sensitivity. This only occurred when CaMCC was expressed before Cx32, suggesting that CaMCC, and by extension CaM, interacts with Cx32 before junction formation. Direct CaM-Cx interaction at junctional and cytoplasmic spots was demonstrated by confocal immunofluorescence microscopy in HeLa cells transfected with Cx32 and in cryosectioned mouse liver. This was confirmed in HeLa cells coexpressing Cx32-GFP (green) and CaM-RFP (red) or Cx32-CFP (cyan) and CaM-YFP (yellow) fusion proteins. Significantly, these cells did not form gap junctions. In contrast, HeLa cells expressing only one of the two fusion proteins (Cx32-GFP, Cx32-CFP, CaM-RFP or CaM-YFP) revealed both junctional and non-junctional fluorescent spots. In these cells, CaM-Cx32 colocalization was demonstrated by secondary immunofluorescent labeling of Cx32 in cells expressing CaM-YFP or CaM in cells expressing Cx32-GFP. CaM-Cx colocalization was further demonstrated at rat liver gap junctions by Freeze-fracture Replica Immunogold Labeling (FRIL).
General practitioner work analysis. (1980)

A wide range of activity at the primary health care level is presented which shows the types of doctor-patient contacts made over the period of one year, the associated morbidity and the action taken by the doctor. A comparison with other similar studies is made.
Full Photonic Band Gap for Surface Modes in the Visible (1996)

We report the first observation of a full photonic band gap for surface modes. An experimental study has been made of the propagation of surface plasmon polaritons on a silver surface that is textured with a hexagonal array of dots with a periodicity of 300 nm. We find that propagation is prohibited in all directions for modes with energies between 1.91 and 2.00 eV.
An End-to-End Protocol for Real Time Computer Networks for Safety Applications (2005)

A fail safe end-to-end protocol is one that delivers all packets ordered and without duplication [1,2]. Such a protocol for real time computer networks incorporating message priority levels is presented in this paper. The model, formal specifications and simulation results on a 8 node transputer network are presented in this paper.
Temporal Transcriptome and Promoter Architecture of the African Swine Fever Virus (2019)

Abstract The African Swine Fever Virus causes haemorrhagic fever in domestic pigs and presents the biggest global threat to animal farming in recorded history. Despite its importance, very little is known the mechanisms and temporal regulation of transcription in ASFV. Here we report the first detailed viral transcriptome analysis of ASFV during early and late infection of Vero cells. In addition to total RNA sequencing, we have characterised the transcription start sites and transcription termination sites at nucleotide-resolution, revealing the distinct DNA consensus motifs of early and late promoters, as well as the sequence determinants for transcription termination. ASFV can utilise alternative promoters to generate distinct proteins from the same transcription unit that differ with respect to the polypeptide N-terminus. Finally, our results reveal that the ASFV-RNAP undergoes transcript slippage at the 5’ end of transcription units that in a promoter sequence-specific manner results in the addition of 5’-AT and 5’-ATAT tails to mRNAs.
Cyclopentadienylmetal trichloride formation versus metallocene dichloride formation in the reactions of silylated cyclopentadienes with zirconium and hafnium chlorides. Crystal structure of (1,3-bis(trimethylsilyl)cyclopentadienyl)titanium trichloride (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCyclopentadienylmetal trichloride formation versus metallocene dichloride formation in the reactions of silylated cyclopentadienes with zirconium and hafnium chlorides. Crystal structure of (1,3-bis(trimethylsilyl)cyclopentadienyl)titanium trichlorideCharles H. Winter, Xiao Xing Zhou, Daniel A. Dobbs, and Mary Jane HeegCite this: Organometallics 1991, 10, 1, 210–214Publication Date (Print):January 1, 1991Publication History Published online1 May 2002Published inissue 1 January 1991https://pubs.acs.org/doi/10.1021/om00047a051https://doi.org/10.1021/om00047a051research-articleACS PublicationsRequest reuse permissionsArticle Views882Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Rapid Intrahost Evolution of Human Cytomegalovirus Is Shaped by Demography and Positive Selection (2013)

Populations of human cytomegalovirus (HCMV), a large DNA virus, are highly polymorphic in patient samples, which may allow for rapid evolution within human hosts. To understand HCMV evolution, longitudinally sampled genomic populations from the urine and plasma of 5 infants with symptomatic congenital HCMV infection were analyzed. Temporal and compartmental variability of viral populations were quantified using high throughput sequencing and population genetics approaches. HCMV populations were generally stable over time, with ,88% of SNPs displaying similar frequencies. However, samples collected from plasma and urine of the same patient at the same time were highly differentiated with approximately 1700 consensus sequence SNPs (1.2% of the genome) identified between compartments. This intercompartment differentiation was comparable to the differentiation observed in unrelated hosts. Models of demography (i.e., changes in population size and structure) and positive selection were evaluated to explain the observed patterns of variation. Evidence for strong bottlenecks (.90% reduction in viral population size) was consistent among all patients. From the timing of the bottlenecks, we conclude that fetal infection occurred between 13-18 weeks gestational age in patients analyzed, while colonization of the urine compartment followed roughly 2 months later. The timing of these bottlenecks is consistent with the clinical histories of congenital HCMV infections. We next inferred that positive selection plays a small but measurable role in viral evolution within a single compartment. However, positive selection appears to be a strong and pervasive driver of evolution associated with compartmentalization, affecting $34 of the 167 open reading frames (,20%) of the genome. This work offers the most detailed map of HCMV in vivo evolution to date and provides evidence that viral populations can be stable or rapidly differentiate, depending on host environment. The application of population genetic methods to these data provides clinically useful information, such as the timing of infection and compartment colonization.
Efficient Hybrid Inline and Out-of-Line Deduplication for Backup Storage (2014)

Backup storage systems often remove redundancy across backups via inline deduplication, which works by referring duplicate chunks of the latest backup to those of existing backups. However, inline deduplication degrades restore performance of the latest backup due to fragmentation, and complicates deletion of expired backups due to the sharing of data chunks. While out-of-line deduplication addresses the problems by forward-pointing existing duplicate chunks to those of the latest backup, it introduces additional I/Os of writing and removing duplicate chunks. We design and implement RevDedup , an efficient hybrid inline and out-of-line deduplication system for backup storage. It applies coarse-grained inline deduplication to remove duplicates of the latest backup, and then fine-grained out-of-line reverse deduplication to remove duplicates from older backups. Our reverse deduplication design limits the I/O overhead and prepares for efficient deletion of expired backups. Through extensive testbed experiments using synthetic and real-world datasets, we show that RevDedup can bring high performance to the backup, restore, and deletion operations, while maintaining high storage efficiency comparable to conventional inline deduplication.
Application of Topographical Methods To Clinical Esophageal Manometry (2000)

Topographical methods are more accurate than commonly used methods in diagnosing the type of severe motor dysfunction and provide additional information important in the clinical practice of esophageal manometry.
Microanalyse X par sonde électronique - Principe et instrumentation (2009)

La microanalyse X à sonde électronique permet d’analyser le spectre caractéristique de rayons X généré dans une cible par un faisceau d'électrons finement focalisé. Cette spécificité lui confère la qualification de méthode d'analyse chimique à l'échelle du micromètre. Cet article est tout d’abord consacré aux aspects théoriques avec la présentation des interactions électrons-matière et des différentes émissions électroniques et électromagnétiques qui en résultent. Est ensuite détaillé l’aspect instrumentation, de la colonne électronique, aux spectromètres de rayons X, à dispersion ou à sélection, jusqu’au dernier venu parmi les détecteurs semi-conducteur.
Four fibroblast tropomyosin isoforms are expressed from the rat alpha-tropomyosin gene via alternative RNA splicing and the use of two promoters (1991)

cDNA clones encoding four rat tropomyosin isoforms, termed TM-2, TM-3, TM-5a, and TM-5b, were isolated and characterized. All are derived from the alpha-tropomyosin gene via alternative RNA processing and the use of two alternate promoters. The cDNA sequences predict that TM-2 and TM-3 both contain 284 amino acids and differ from each other only at an internal region of the protein from amino acids 189 through 213, due to alternative splicing of exons 6a and 6b. TM-5a and TM-5b both contain 248 amino acids and differ from each other only at an internal exon encoding amino acids 153 through 177, also due to alternative splicing of exons 6a and 6b. The differences in the amino acid sequence encoded by these alternate exons affects the theoretical actin-binding pattern of the tropomyosins, such that TM-5b is expected to bind actin with greater affinity than TM-5a. TM-2 and TM-3 are transcribed from the upstream promoter, and TM-5a and TM-5b are transcribed from an internal promoter. In addition, all four isoforms contain the identical COOH-terminal coding region. RNA protection analyses revealed that the mRNA for each isoform is expressed in a number of different tissues and cell types, although the expression of some isoforms is restricted to particular cell types. Furthermore, the expression of mRNA encoding these isoforms was found to be altered in a number of different virally transformed cell lines. The changes in the expression of tropomyosin mRNAs in transformed cells reflect changes in the relative use of the two promoters, as well as the relative use of alternatively spliced exons 6a and 6b.
Research progress in umbilical cord blood stem cell transplantation for treatment of liver diseases (2011)

Umbilical cord blood stem cells (UCBSC) transplantation is one of the most promising stem cell transplantation.Currently,basic experimental studies of UCBSC in liver diseases have shown that UCBSC can be greatly amplified and induced to differentiate into functional hepatocyte-like cells in vivo and in vitro.The relationship between treatment of UCBSC transplantation and liver diseases has not yet been determined,because there are few relevant studies on clinical application.In this paper,UCBSC transplantation for treatment of liver diseases is reviewed.

Key words: 
Cord blood stem cell transplantation;  Liver diseases;  Therapy
Expression of Transforming Growth Factor-β1, 2, and 3 m RNAand Protein in Human Cancers (1994)

Historically, the role for transforming growth factor-βs (TGF-βs) in malignancy has been difficult to prove. TGF-βs are potent autocrine and paracrine negative regulators of the growth of normal epithelial and neuroectodermal cells in vitro. Therefore we hypothesized that the aberrant growth of tumor cells and neoplastic development, in general, may be due to a loss of growth regulation by TGF-β. By immunohistochemistry, using isoform specific antibodies raised to synthetic peptides of each TGF-β isoform, and by in situ hybridization, using non-cross-hybridizing cRNA probes to each TGF-β isoform, we examined the protein and mRNA expression of TGF-β, respectively, in a variety of carcinomas and gliomas compared to normal tissue. The analysis of the cell types that synthesized the messge for TGF-β isoforms compared to those which synthesized the protein indicated whether TGF-β functioned by an autocrine or paracrine mechanism of action in these tumors.
Movement representation in the dorsal and ventral premotor areas of owl monkeys: A microstimulation study (1996)

We used intracortical microstimulation to investigate the lateral premotor cortex and neighboring areas in 14 hemispheres of owl monkeys, focusing on the somatotopic distribution of evoked movements, thresholds for forelimb movements, and the relative representation of proximal and distal forelimb movements. We elicited movements from the dorsal and ventral premotor areas (PMD, PMV), the caudal and rostral divisions of primary motor cortex (M1c, M1r), the frontal eye field (FEF), the dorsal oculomotor area (OMD; area 8b), the supplementary motor area (SMA), and somatosensory cortex (areas 3a and 3b). Area PMD was composed of architectonically distinguishable caudal and rostral subdivisions (PMDc, PMDr). Stimulation of PMD elicited movements of the hindlimb, forelimb, neck and upper trunk, face, and eyes. Hindlimb and forelimb movements were represented in the caudalmost part of PMDc. Face, neck, and eye movements were represented in the lateral and rostral parts of PMDc and in PMDr. Stimulation of PMV elicited forelimb and orofacial movements, but not hindlimb movements. Both proximal and distal forelimb movements were elicited from PMDc and PMV, although PMD stimulation elicited mainly shoulder and elbow movements, while PMV stimulation evoked primarily wrist and digit movements. Distal movements were evoked more frequently from PMV than from M1r or M1c. Across cases, the median forelimb thresholds for PMDc and PMV were 60 and 36 microA, respectively, values that differ significantly from each other and from the value of 11 microA obtained for M1r. Our observations indicate that premotor cortex is much more responsive to electrical stimulation than commonly thought, and contains a large territory from which eye movements can be elicited. These results suggest that in humans, much of the electrically excitable cortex located on the precentral gyrus, including cortex sometimes considered part of the frontal eye field, is probably homologous to the premotor cortex of nonhuman primates.
Robust real-time tracking combining 3D shape, color, and motion (2015)

Real-time tracking algorithms often suffer from low accuracy and poor robustness when confronted with difficult, real-world data. We present a tracker that combines 3D shape, color (when available), and motion cues to accurately track moving objects in real-time. Our tracker allocates computational effort based on the shape of the posterior distribution. Starting with a coarse approximation to the posterior, the tracker successively refines this distribution, increasing in tracking accuracy over time. The tracker can thus be run for any amount of time, after which the current approximation to the posterior is returned. Even at a minimum runtime of 0.37 ms per object, our method outperforms all of the baseline methods of similar speed by at least 25% in root-mean-square (RMS) tracking error. If our tracker is allowed to run for longer, the accuracy continues to improve, and it continues to outperform all baseline methods. Our tracker is thus anytime, allowing the speed or accuracy to be optimized based on the needs of the application. By combining 3D shape, color (when available), and motion cues in a probabilistic framework, our tracker is able to robustly handle changes in viewpoint, occlusions, and lighting variations for moving objects of a variety of shapes, sizes, and distances.
Ti/sup 3+/ ion concentration and Ti:sapphire laser performance (1997)

The pulsed and CW laser performance of a range of titanium sapphire laser crystals is reported. Titanium ion concentration in the crystals ranged from 0.07 at.% to 0.25 and 0.41 at.% for some recently developed heavily doped crystals. Values of the crystal figure of merit lay between 30 and 220. Threshold, slope efficiency, tunability, and temporal response are assessed. Comparisons are made with the predictions of a simple rate equation model.
Component Wear in Coal-Fueled Diesel Engines (1989)

Practical use of coal-fueled diesel engines depends on the improvement of component durability. The wear characteristics of standard materials in the coal-fueled engine were studied first. Candidate wear-resistant materials were sorted by bench-scale tests. The best material combinations were applied to small-scale engine tests for operation on seeded diesel fuel. Components with hard materials and coatings were scaled up for coal-water mixture testing on the locomotive engine. Results indicate practical solutions for ring and liner wear and positive progress toward defining the material requirements for the fuel injection nozzle.
Susceptibility of Nonpromoter CpG Islands to De Novo Methylation in Normal and Neoplastic Cells (2001)

The methylation patterns that we observed suggest that exonic CpG islands are more susceptible to de novo methylation than promoter islands and that methylation may be seeded in exonic regions, from which it can spread to other islands, including promoter regions. Subsequent selection of cells with a growth advantage conferred by spread of methylation into and inactivation of a particular promoter might then contribute to the genesis of a specific type of cancer.
Einleitung (1998)

In der vorliegenden Untersuchung wird im Rückgriff auf einen diskursanalytischen Ansatz die öffentliche Diskussion über das ‚Hausmüllproblem‘ in der Bundesrepublik Deutschland und in Frankreich im Zeitraum von 1970–1995 vergleichend analysiert. Die Arbeit ist in acht Kapitel gegliedert. Das erste Kapitel stellt den theoretischen Bezugsrahmen der Untersuchung vor. Dabei wird die Bedeutung und Entwicklung der Praxis des industriegesellschaftlichen Umgangs mit Abfällen diskutiert. Die öffentlichen Auseinandersetzungen über den ‚angemessenen‘ Umgang mit Abfällen werden in einer Theorie der Institutionalisierung ökologischer Kommunikation verortet. Daran anschließend wird im zweiten Kapitel die diskursanalytische Perspektive der Arbeit entwickelt. Der konzeptuelle Rahmen und das konkrete methodische Vorgehen werden erläutert. Während der Umgang mit Abfällen den allgemeinen thematischen Bezugspunkt der Untersuchung darstellt, ist die Medienberichterstattung der spezifische Ort, an dem dieses Thema untersucht wird. Im dritten Kapitel werden deswegen die Ergebnisse medien- und kommunikationswissenschaftlicher Analysen der massenmedialen Umweltberichterstattung mit einer Fokussierung auf den deutsch-französischen Vergleich diskutiert und die vorliegende Arbeit in dem breiteren Spektrum von Medienanalysen verortet. Im vierten Kapitel wird die gesellschaftliche Infrastrukturgeschichte des Umgangs mit Müll seit dem Mittelalter vorgestellt. Der räumliche Fokus liegt auf dem Aufbau der kommunalen Entsorgung in Deutschland und Frankreich.
Relative transfer function modeling for supervised source localization (2013)

Speaker localization is one of the most prevalent problems in speech processing. Despite significant efforts in the last decades, high reverberation level still limits the performance of localization algorithms. Furthermore, using conventional localization methods, the information that can be extracted from dual microphone measurements is restricted to the time difference of arrival (TDOA). Under far-field regime, this is equivalent to either azimuth or elevation angles estimation. Full description of speaker's coordinates necessitates several microphones. In this contribution we tackle these two limitations by taking a manifold learning perspective for system identification. We present a training-based algorithm, motivated by the concept of diffusion maps, that aims at recovering the fundamental controlling parameters driving the measurements. This approach turns out to be more robust to reverberation, and capable of recovering the speech source location using merely two microphones signals.
Vol. 42, Suplemento 1 (2017)

Los pólenes de gramíneas y su relación con alergias en el Neotrópico: el caso de Costa Rica Contiene los siguientes artìculos:POLLEN OF GRAMINEAE END ITS BOTANICAL, TAXONOMICAL AND ECOLOGICAL ASPECTS POLLEN OF GRAMINEAE AND ITS RELATION WUTH ALLERGIC EFECTS IN COSTA RICATYPE-I HYPERSENSITIVITY TO GAMINEAE POLLENS (BY TRIBE) IN ALLERGIC RHINITIS PATIENTSTIPE-I HYPERSENSITIVTY TO POLLEN OF THE POACEAE FAMILY (GRAMINEAE), BY TRIBE, IN ATOPIC DERMATITIS PATIENTSTIPE-I HYPERSENSITIVITY TO GRAMINEAE POLLEN (BY TRIBE) IN ALLERGIC CONJUNTIVITIS PATIENTSTIPE-I HYPERSENSITIVITY TO GRAMINEAE POLLEN (BY TRIBE) IN BRONCHIAL ASTHMA PATIENTS TIPE-I HYPERSENSITIVITY TO GRAMINEAE POLLENS (BY SPECIES) IN ALLERGIC RHINITIS PATIENTSALLERGY POSITIVITY CORRELATIONS OF GRAMINEAE (TRIBES AND SPECIES)POLEN DE GRAMINEAS Y SUS ASPECTOS BOTANICO, TAXONOMICOS Y ECOLOGICOS LOS POLENES DE GRAMINEAS Y SU RELACION CON MANIFESTACIONES ALERGICAS EN COSTA RICA HIPERSENSIBILIDAD TIPO I A POLENES DE GRAMINEAS ORDENADOS POR TRIBU, EN PACIENTES CON RINITIS ALERGICAHIPERSENSIBILIDAD TIPO I A POLENES DE LA FAMILIA POACEAE (GRAMINEAE) ORDENADOS POR TRIBU, EN PACIENTES CON DERMITITIS ATOPICA HIPERSENSIBILIDAD TIPO I A POLENES DE GRAMINEAS (POR TRIBU), EN PACIENTES CON CONJUNTIVITIS ALERGICAHIPERSENSIBILIDAD TIPO I A POLENES DE GRAMINEAS (POR TRIBU), EN PACIENTES CON ASMA BRONQUIALHIPERSENSIBILIDAD TIPO I A POLENES DE GRAMINEAS (POR ESPECIE) EN PACIENTES CON RINITIS ALERGICA CORRELACION DE POSIBILIDADES ALERGICAS PARA LAS PRUEBAS DE GRAMINEAS ENTRE TRIBUS Y ESPECIES