gebhart/patrepeval huggingface dataset:
Some chemical and bacteriological effects of various kinds and amounts of lime on certain southern Iowa soils (1932)

Extensive investigations have been conducted to determine the influence of lime on acid soils, but there is still much to be learned regarding the reactions involved and the effects of additions of various kinds and amounts of lime on different soils. Soils vary widely in physical, chemical and bacteriological properties, and hence lime rarely has the same effect on different types. It has been noted that soils with practically the same hydrogen ion concentration may require different amounts of lime to neutralize the acidity. The organic matter and colloidal content are quite variable and will affect not only the need of soils for lime but also the reactions which occur as a result of the treatment.
Analysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constants (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAnalysis of long-chain free fatty acid binding to bovine serum albumin by determination of stepwise equilibrium constantsJohn E. Fletcher, Arthur A. Spector, and J. Douglas AshbrookCite this: Biochemistry 1971, 10, 17, 3229–3232Publication Date (Print):August 1, 1971Publication History Published online1 May 2002Published inissue 1 August 1971https://pubs.acs.org/doi/10.1021/bi00793a011https://doi.org/10.1021/bi00793a011research-articleACS PublicationsRequest reuse permissionsArticle Views1162Altmetric-Citations203LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Calprotectin S100A9 Calcium-binding Loops I and II Are Essential for Keratinocyte Resistance to Bacterial Invasion (2009)

Epithelial cells expressing calprotectin, a heterodimer of S100A8 and S100A9 proteins, are more resistant to bacterial invasion. To determine structural motifs that affect resistance to bacterial invasion, mutations were constructed in S100A9 targeting the calcium-binding loops I and II (E36Q, E78Q, E36Q,E78Q) and the C terminus (S100A9(1-99) and S100A9(1-112)), which contains putative antimicrobial zinc-binding and phosphorylation sites. The S100A8 and mutated S100A9 encoding plasmids were transfected into calprotectin-negative KB carcinoma cells. All transfected cells (except KB-sham) expressed 27E10-reactive heterodimers. In bacterial invasion assays with Listeria monocytogenes and Salmonella enterica serovar Typhimurium (Salmonella typhimurium), cell lines expressing S100A8 in complex with S100A9E36Q, S100A9E78Q, S100A9(1-99), or S100A9(1-112) mutants or the S100A9(1-114) (full-length) calprotectin resisted bacterial invasion better than KB-sham. When compared with KB-S100A8/A9(1-114), cells expressing truncated S100A9(1-99) or S100A9(1-112) with S100A8 also showed increased resistance to bacterial invasion. In contrast, glutamic acid residues 36 and 78 in calcium-binding loops I and II promote resistance in epithelial cells, because cells expressing S100A9E36Q,E78Q with S100A8 were unable to resist bacterial invasion. Mutations in S100A9 E36Q, E78Q were predicted to cause loss of the calcium-induced positive face in calprotectin, reducing interactions with microtubules and appearing to be crucial for keratinocyte resistance to bacterial invasion.
Thrombolytic Therapy (2002)

The therapeutic use of thrombolytic agents is the natural result of the increasing understanding of the pathophysiologic mechanisms underlying normal and deranged thrombosis and fibrinolysis. Plasminogen activators capable of increasing the production of plasmin exhibit considerable efficacy in the treatment of a variety of arterial and venous thrombotic disorders. The ideal thrombolytic agent has yet to be developed but the desired clinical result of rapid opening of the thrombosed vessel without reocclusion, without activation of systemic fibrinogenolysis, and without a risk of hemorrhage is well defined. Clinical studies clearly demonstrate that the addition of a variety of adjunctive agents to the available thrombolytics enhances benefit without inordinate risk. The addition of intravascular angioplasty and stenting to thrombolysis increases the potential long-term benefit. Newer thrombolytic agents and new protocols for the use of existing therapies offer the promise of saving many who would otherwise succumb to coronary or cerebral arterial thrombosis or to venous thromboembolism.
The beneficial effects of modest weight loss on cardiovascular risk factors. (1997)

A range of risk factors affect cardiovascular morbidity and mortality. There is increasing evidence that moderate weight loss, expressed as 5-10% weight loss, has beneficial effects on these cardiovascular risk factors. Such moderate weight loss, combined with exercise, significantly decreases triglyceride levels, while significantly increasing levels of high density lipoprotein (HDL)-cholesterol. Studies also indicate that the oxidizability of lipids and lipoproteins in a given individual is of importance in assessing cardiovascular risk. Preliminary data suggest that moderate weight loss can improve the oxidizability status of obese individuals. Lipoprotein (a) levels, however, are not reduced by modest weight reduction, unless pretreatment values are considered. In addition, there is evidence that limited weight loss (< 7 kg) decreases levels of the haemostatic factors, factor VII and PAI, while increasing the tissue plasminogen activator levels. Fibrinogen levels, however, seem not to benefit from such limited weight loss. Modest weight loss has also been found to cause regression of coronary arterial lesions, and significantly reduce cardiac events, total cardiac mortality, and total mortality. Despite the limited success of long-term weight reduction programmes, it seems worthwhile to motivate overweight people to attempt even a modest amount of weight loss, since this may help to reduce cardiovascular risk factors and total mortality.
HOMOGENEOUS HYDROGENATION OF ALDEHYDES TO ALCOHOLS CATALYZED BY RuCl2(PPh3)3 (1977)

Abstract RuCl2(PPh3)3 was found to be a good catalyst for the hydrogenation of aliphatic aldehydes to alcohols under mild conditions. Aromatic aldehydes were reduced to benzyl alcohols without hydrogenolysis. Ketones and nitro groups were not reduced under the same conditions.
Methane Activation by Metal‐Free Radical Cations: Experimental Insight into the Reaction Intermediate (2009)

A precise jab to methane: The SO(2)(*+) radical cation (see figure) effectively activates CH(4) at room temperature through a [H(3)C(*)...HOSO(+)] methyl intermediate isolated in the gas phase by mass spectrometry. Methanol and ionized methyl hydrogen sulfoxylate, CH(3)OSOH(*+), are formed by selective, direct attack of the incipient methyl radical at the O atom of the intermediate. The reaction shows radical and charge effects in the activation of methane by metal-free radical cations.
Self-Aware Smart Contracts with Legal Relevance (2018)

The following topics are dealt with: learning (artificial intelligence); neural nets; pattern classification; feature extraction; feedforward neural nets; convolution; image classification; recurrent neural nets; pattern clustering; support vector machines.
Integrating object-oriented scripting languages with HyTime (1994)

HyTime provides a comprehensive set of primitives for composing hypermedia documents, but does not provide facilities for representing interaction or dynamic behavior, areas which are required in commercial multimedia authoring environments. In previous work we have developed and implemented a prototype HyTime engine called HyOctane in which HyTime interactive multimedia documents can be stored and retrieved. We extend this work to include scripting language facilities, an area currently not dealt with by the standard but which is crucial in order to represent interactive multimedia documents. We compare different approaches to integrating HyTime document type definitions with scripts and describe the extensions to our engine architecture and implementation status.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Photohydrate‐Mediated Reactions of Uridine, 2′‐Deoxyuridine and 2′‐Deoxycytidine with Amines at Near Neutral <scp>pH</scp> (2013)

Photohydrates are formed in high yield when uridine (Urd), 2'-deoxyuridine (dUrd), cytidine (Cyd) and 2'-deoxycytidine (dCyd) are irradiated with UVC in aqueous solution. The thermal reactions of the photohydrates of Urd with amines at pH values near pH 7.5 have been studied using UV spectroscopy, HPLC, mass spectrometry and, in some cases, NMR. It has been found that a number of amines (i.e. ethylenediamine, N,N'-dimethylethylenediamine, glycine, glycinamide, glycylglycine, glycylgylcylglycine, putrescine, spermidine and spermine) react thermally with such hydrates to form products with UV spectra characteristic of opened ring uridine-amine adducts. In general, these products display a strong absorption peak with λmax in the range between 288 and 310 nm. Mass spectral studies of a number of the products indicate that they contain one molecule of parent nucleoside and one molecule of reactant amine. Upon standing in water these products revert to parent hydrate, while heating produces parent nucleoside. Less comprehensive studies indicate that photohydrates of dUrd and dCyd undergo analogous thermal reactions. Preliminary results suggest that UV-irradiated polyuridylic acid and polycytidylic acid undergo similar reactions. These results may have relevance for obtaining a complete understanding of the biological effects of producing Urd and dCyd photohydrates in a cellular environment.
Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis (2010)

Massive Mutagenesis is a proprietary library creation method that enables the fast generation of high-quality genetic libraries. Starting from a single gene on a plasmid and hundreds to thousands of oligonucleotides, a one-step single-strand circular amplification method creates random combinations of several site-directed substitutions, insertions, or deletions. Libraries of up to a billion such variants have been routinely generated. Sequencing those variants demonstrated lower biases than alternative approaches such as error-prone PCR. Screening and selecting them has yielded improved biocatalysts, therapeutic proteins, and antibodies.
Correcting Drift, Head and Body Misalignments between Virtual and Real Humans (2014)

Head-mounted displays (HMD) allow a personal and immersive viewing of virtual environments, and can be used with almost any desktop computer. Most HMDs have inertial sensors embedded for tracking the user head rotations. These low-cost sensors have high quality and availability. However, even if they are very sensitive and precise, inertial sensors work with incremental information, easily introducing errors in the system. The most relevant is that head tracking suffers from drifting. In this paper we present important limitations that still prevent the wide use of inertial sensors for tracking. For instance, to compensate for the drifting, users of HMD-based immersive VEs move away from their suitable pose. We also propose a software solution for two problems: prevent the occurrence of drifting in incremental sensors, and avoid the user from move its body in relation to another tracking system that uses absolute sensors (e.g. MS Kinect). We analyze and evaluate our solutions experimentally, including user tests. Results show that our comfortable pose function is effective on eliminating drifting, and that it can be inverted and applied also to prevent the user from moving their body away of the absolute sensor range. The efficiency and accuracy of this method makes it suitable for a number of applications in immersive VR.
Lymphocyte specificity to protein antigens. I. Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice. (1977)

An in vitro assay which measures antigen-induced proliferation of primed murine lymph node cells is described. The response is mediated by T eclls since it can be obtained by using nylon wool-passed lymphocytes (less than 1% Ig+ cells) and it can be abolished by treatment with anti-Thy 1.2 and C. Furthermore, LN cells from nu/nu mice injected with antigen do not demonstrate antigen-induced proliferation in contrast to the response observed in euthymic littermate controls. Other relevant parameters of this proliferative assay include the observations that the response is highly antigen specific, can be seen as early as 4 days and as late as 60 days after in vivo priming, is restricted to the use of certain sets of LN when animals are injected subcutaneously at the base of the tail, and can be seen with LN cells from mice primed with antigen in either CFA or ICFA. The ease of the assay coupled with its specificity and quantitative dimensions provides a direct and simple method to evaluate processes involved in antigen-induced murine T lymphocyte activation.
Electronic Properties of Antiperovskite Materials from State‐of‐the‐Art Density Functional Theory (2015)

We present a review on the research developments on the theoretical electronic properties of the antiperovskite materials. The antiperovskite materials have perovskite type structure with the positions of cations and anions interchanged. The electronic structures are used to explain different physical properties of materials; therefore it is crucial to understand band structures and densities of states of materials for their effective use in technology. The theoretical results of the electronic structure of antiperovskites were discussed and compared with the available experimental results to measure the accuracy of the research done so far on these materials. The important physical properties of these compounds like magnetic properties and superconductivity are also highlighted. Nevertheless the thermoelectric properties of these materials are still unexplored except for a few reports which suggest that antiperovskite materials may be potential candidates for thermoelectric generators.
Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates (2013)

In 2001, researchers at the University of California, Los Angeles, described the isolation of a new population of adult stem cells from liposuctioned adipose tissue that they initially termed Processed Lipoaspirate Cells or PLA cells. Since then, these stem cells have been renamed as Adipose-derived Stem Cells or ASCs and have gone on to become one of the most popular adult stem cells populations in the fields of stem cell research and regenerative medicine. Thousands of articles now describe the use of ASCs in a variety of regenerative animal models, including bone regeneration, peripheral nerve repair and cardiovascular engineering. Recent articles have begun to describe the myriad of uses for ASCs in the clinic. The protocol shown in this article outlines the basic procedure for manually and enzymatically isolating ASCs from large amounts of lipoaspirates obtained from cosmetic procedures. This protocol can easily be scaled up or down to accommodate the volume of lipoaspirate and can be adapted to isolate ASCs from fat tissue obtained through abdominoplasties and other similar procedures.
Mold deformation in nanoimprint lithography (2004)

In nanoimprint lithography (NIL), one of the key points to be addressed is the printing uniformity on large area. During the process, the silicon mold undergoes significant mechanical stress of different kinds (tension, compression, flexion, and torsion). These stresses are function of the mold design and appear under the concurrent influence of both the applied pressure on the backside of the mold and an opposite force due to the polymer viscoelastic behavior. This translates into non-negligible deformations within patterned or unpatterned zones. This is a major issue because it causes nonuniformity of the printing, mold pattern break and degradation of the polymer surface. In this article, we demonstrate that during the imprint process mold deformations really occur at the local scale of the patterns but also at a larger scale.
Fall bulbs 1930 / (1930)

The heights of the flowers are given in inches.Varieties marked with an asterisk (*) are good for forcing.Not less than 6 bulbs sold at dozen rate, 25 bulbs at 100 rate T^ink and Rose Shades Doz. 100 Afterglow.A rich, deep salmon orange, salmon border.A striking variety.26 in.$1.40 $9.00 Baronne de la Tonnaye.Intense carmine pink with light rose margin; white base.26 in.85 5.50 Centenaire.Exquisite deep violet rose, shading lighter at edges.Magnificent.32 in.90 6.00 Clara Butt.Pastel salmon pink flushed deeper.One of the most deservedly popu¬ lar.21 in.80 5.00 Flamingo.An attractive, deep coral or shell pink.A superb color.26 in...... .1.10 7.00 La Fiancee.Brilliant deep rose shading lighter at edges.Fine for borders or forc¬ ing. 25 in.. .1.50 10.00 Le Notre.An early blooming type with clear light pink blooms.22 in... 1.10 7.00 Mme.Krelage.Soft rose pink toning down to pale silvery rose at edges; large flowers, 24 in.85 5.50 Massachusetts.Large, vivid pink flowers with white center.An outstanding va¬ riety for bedding.26 in..90 6.00 Princess Elizabeth (Julie Vinot).Soft cattleya or orchid pink with white base.23 in.90 6.00 Professor Rauwenhoff.Massive blooms of deep cherry red, shaded salmon inside, with lighter center.28 in.._. 1.00 6.50 Psyche.An exceptional varietv with very beautiful flowers of salmon rose color.28 in.90 6.00 Queen Mary.A rich, self colored rose with light blue base.28 in.1.30 8.00 Roi d'Islande.A remarkably rich and unusual shade of old rose.Splendid forcer.26 in.1.40 9.00
Pterandra viridiflora (Malpighiaceae), a new species from Brazil (2005)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
MOLECULAR AND CYTOGENETIC CHARACTERIZATION OF A METALLOTHIONEIN GENE OF DROSOPHILA (1986)

A chromosomal DNA segment containing the metallothionein gene was isolated from a genomic library of Drosophila melanogaster using a previously characterized cDNA of this species as a probe. A segment of 1543 base pair (bp) was sequenced and found to include the cDNA sequence interrupted by one small intron. Several lines of evidence indicate that there is a single copy of the metallothionein gene (Mtn) in Drosophila; any other related genes, if they occur, must be sufficiently different that they are not detectable by our probe, even under hybridization conditions of reduced stringency. According to in situ hybridization and deletion mapping, Mtn is located in the right arm of the third chromosome in region 85E10-15. Within 300 bases upstream of the apparent site of transcription initiation, there are several short intervals very similar to the 12-bp segments considered to be responsible for metal regulation in mammalian systems.
Formation and rearrangement of adducts from benzyne and substituted 2,1,3-benzoselenadiazoles (1981)

A series of 5-(1,2-benzoselenazol-3-yl)pentadienonitrile derivatives (2) has been prepared by addition of benzyne to substituted 2,1,3-benzoselenadiazoles. Some of these adducts rearrange either thermally or photochemically to give 2-(2-pyridyl)phenyl selenocyanates (7), which are reduced to 2-phenylpyridine derivatives (6) or hydrolysed to give ultimately, diselenides (9). The crystal structure of one benzyne adduct (2b) is reported and the mechanism of its rearrangement discussed.
Spo13 protects meiotic cohesin at centromeres in meiosis I (2002)

In the absence of Spo13, budding yeast cells complete a single meiotic division during which sister chromatids often separate. We investigated the function of Spo13 by following chromosomes tagged with green fluorescent protein. The occurrence of a single division in spo13Delta homozygous diploids depends on the spindle checkpoint. Eliminating the checkpoint accelerates meiosis I in spo13Delta cells and allows them to undergo two divisions in which sister chromatids often separate in meiosis I and segregate randomly in meiosis II. Overexpression of Spo13 and the meiosis-specific cohesin Rec8 in mitotic cells prevents separation of sister chromatids despite destruction of Pds1 and activation of Esp1. This phenotype depends on the combined overexpression of both proteins and mimics one aspect of meiosis I chromosome behavior. Overexpressing the mitotic cohesin, Scc1/Mcd1, does not substitute for Rec8, suggesting that the combined actions of Spo13 and Rec8 are important for preventing sister centromere separation in meiosis I.
Biosynthesis of basement membrane collagen by rabbit corneal endothelium in vitro. (1976)

The synthesis of collagen has been demonstrated in endothelial cells of Descemet's membrane isolated from rabbit cornea. Incorporation of [14C]proline and [14C]lysine into nondialyzable protein was measured in the medium and cell fraction after incubating Descemet's membrane for up to 5 hours. In the [14C]collagen synthesized by the endothelium, 15% of the hydroxy[14C]proline was present as the 3-isomer. About 98% of the hydroxy[14C]lysine in the 14C-labeled-protein found in the medium was glycosylated; 95% of the glycosylated hydroxy[14C]lysine was in the form of the disaccharide glucosyl-galactosyl-hydroxy[14C]lysine. Time course experiments with [14C]proline indicated that there was a delay of about 60 min before significant amounts of [14C]collagen were secreted into the medium. The initial polypeptides of [14C]collagen synthesized by the corneal endothelium had an apparent molecular weight of 155,000. The chemical and physical properties of the [14C]collagen synthesized by rabbit corneal endothelium are consistent with those of basement membrane collagen synthesized by other cell types.
Membrane-associated farnesylated UCH-L1 promotes α-synuclein neurotoxicity and is a therapeutic target for Parkinson's disease (2009)

Ubiquitin C-terminal hydrolase-L1 (UCH-L1) is linked to Parkinson's disease (PD) and memory and is selectively expressed in neurons at high levels. Its expression pattern suggests a function distinct from that of its widely expressed homolog UCH-L3. We report here that, in contrast to UCH-L3, UCH-L1 exists in a membrane-associated form (UCH-L1(M)) in addition to the commonly studied soluble form. C-terminal farnesylation promotes the association of UCH-L1 with cellular membranes, including the endoplasmic reticulum. The amount of UCH-L1(M) in transfected cells is shown to correlate with the intracellular level of alpha-synuclein, a protein whose accumulation is associated with neurotoxicity and the development of PD. Reduction of UCH-L1(M) in cell culture models of alpha-synuclein toxicity by treatment with a farnesyltransferase inhibitor (FTI-277) reduces alpha-synuclein levels and increases cell viability. Proteasome function is not affected by UCH-L1(M), suggesting that it may negatively regulate the lysosomal degradation of alpha-synuclein. Therefore, inhibition of UCH-L1 farnesylation may be a therapeutic strategy for slowing the progression of PD and related synucleinopathies.