gebhart/patrepeval huggingface dataset:
Content Adaptive Mesh Representation of Images Using Binary Space Partitions (2009)

The interest in content adaptive mesh generation of images has been arising lately due to its wide area of applications in image processing. The major issue is to represent an image with a low number of pixels while preserving its content. These pixels or the nonuniform samples are then used to generate a mesh that approximates the corresponding image. This work presents a novel method based on Binary Space Partitions in combination with three clustering schemes to approximate an image with a mesh. The algorithm has the ability to simultaneously reduce the number of pixels and generate the mesh approximation. The idea is to assume each triangle of the mesh as a plane. Consequently, it will be possible to reconstruct the inlying pixels with planar equations defined from the three nodes of each triangle. If a triangle's equation does not have the ability to reconstruct the pixels lying within up to a predefined error, it is split into two new triangles. Tested on several real images, the proposed method leads to reduced size meshes in a fast manner while retaining the visual quality of the reconstructed images. In addition, it is parallelizable due to the property of Binary Space Partitions which facilitates its application in real-time scenarios.
An Extended Compact Tension Specimen for Fatigue Crack Propagation and Fracture (1996)

An extended compact tension specimen, EC(T) has been developed for fatigue and fracture testing. Documented herein are stress-intensity factor and compliance expressions for the EC(T) specimen.
P-Type Calcium Channels and Transmitter Release From Nerve Terminals (1994)

Different types of Ca2+ channels have been identified in neuronal soma. P-type Ca2+ channels resistant to the blocking effect of dihydropyridines and w-conotoxin-GVIA but sensitive to the funnel web spider toxin and w-agatoxin-IVA mediate the influx of Ca2+ involved in transmitter release in mammalian synapses.
Inexpensive laser luminescence decay time apparatus (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInexpensive laser luminescence decay time apparatusJ. N. Demas and C. M. FlynnCite this: Anal. Chem. 1976, 48, 2, 353–357Publication Date (Print):February 1, 1976Publication History Published online1 May 2002Published inissue 1 February 1976https://pubs.acs.org/doi/10.1021/ac60366a012https://doi.org/10.1021/ac60366a012research-articleACS PublicationsRequest reuse permissionsArticle Views58Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Purification of a multiprotein complex containing centrosomal proteins from the Drosophila embryo by chromatography with low-affinity polyclonal antibodies. (1992)

A 190-kDa centrosomal protein interacts with microtubules when Drosophila embryo extracts are passed over microtubule-affinity columns. We have obtained a partial cDNA clone that encodes this protein. Using a fusion protein produced from the clone, we have developed a novel immunoaffinity chromatography procedure that allows both the 190-kDa protein and a complex of proteins that associates with it to be isolated in in a single step. For this procedure, the fusion protein is used as an antigen to prepare rabbit polyclonal antibodies, and those antibodies that recognize the 190-kDa protein with low affinity are selectively purified on a column containing immobilized antigen. These low-affinity antibodies are then used to construct an immunoaffinity column. When Drosophila embryo extracts are passed over this column, the 190-kDa protein is quantitatively retained and can be eluted in nearly pure form under nondenaturing conditions with 1.5 M MgCl2, pH 7.6. The immunoaffinity column is washed with 1.0 M KCl just before the elution with 1.5 M MgCl2. This wash elutes 10 major proteins, as well as a number of minor ones. We present evidence that these KCl-eluted proteins represent additional centrosomal components that interact with the 190-kDa protein to form a multiprotein complex within the cell.
Ascorbic acid determination with an automated enzymatic procedure (1997)

Given the widespread interest in antioxidant nutrients, we have developed a new procedure that will permit the automated determination of plasma ascorbic acid (AA) concentration with a Roche Fara centrifugal analyzer. After the deproteinization of plasma with metaphosphoric acid, AA is oxidized to dehydroascorbic acid by AA oxidase. The product is coupled to o-phenylenediamine to produce a chromophore for which the absorbance is measured at 340 nm. The procedure allows much faster throughput than conventional HPLC methods while yielding results that correlate well and provide improved precision. Linearity extends beyond the reference range of 26.1-84.6 micromol/L, and severe hemolysis is the only interference identified.
Pertussis in Poland (2004)

As vaccination coverage did not decrease and diagnostics have not been improved since the 1980s, it is possible that waning immunity and the appearance of Bordetella pertussis vaccine escape mutants are involved in the changing pertussis epidemiological parameters. Further monitoring studies, together with improving diagnostics, might allow more precise epidemiological data to be obtained. An additional booster dose of acellular pertussis vaccine at age 6 years has been included in the current vaccination schedule.
Up-Regulation of the<i>Yersinia enterocolitica yop</i>Regulon by Deletion of the Flagellum Master Operon<i>flhDC</i> (2002)

The Yop virulon enables extracellularly located Yersinia, in close contact with a eukaryotic target cell, to inject bacterial toxic proteins directly into the cytosol of this cell. Several Ysc proteins, forming the Yop secretion apparatus, display homology with proteins of the flagellar basal body. To determine whether this relationship could extend to the regulatory pathways, we analyzed the influence of flhDC, the master regulatory operon of the flagellum, on the yop regulon. In an flhDC mutant, the yop regulon was up-regulated. The transcription of virF and the steady-state level of the transcriptional activator VirF were enhanced. yop transcription was increased at 37 degrees C and could also be detected at a low temperature. Yop secretion was increased at 37 degrees C and occurred even at a low temperature. The Ysc secretion machinery was thus functional at room temperature in the absence of flagella, implying that in wild-type bacteria, FlhD and/or FlhC, or the product of a gene downstream of flhDC, represses the yop regulon. In agreement with this notion, increased expression of flhDC in wild-type bacteria resulted in the oversecretion of flagellins at room temperature and in decreased Yop secretion at 37 degrees C.
High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer′s Disease Biomarker Development (2011)

We have established high-throughput lectin-antibody ELISAs to measure different glycans on transferrin (Tf) in cerebrospinal fluid (CSF) using lectins and an anti-transferrin antibody (TfAb). Lectin blot and precipitation analysis of CSF revealed that PVL (Psathyrella velutina lectin) bound an unique N-acetylglucosamine-terminated N-glycans on "CSF-type" Tf whereas SSA (Sambucus sieboldiana agglutinin) bound α2,6-N-acetylneuraminic acid-terminated N-glycans on "serum-type" Tf. PVL-TfAb ELISA of 0.5 μL CSF samples detected "CSF-type" Tf but not "serum-type" Tf whereas SSA-TfAb ELISA detected "serum-type" Tf but not "CSF-type" Tf, demonstrating the specificity of the lectin-TfAb ELISAs. In idiopathic normal pressure hydrocephalus (iNPH), a senile dementia associated with ventriculomegaly, amounts of the SSA-reactive Tf were significantly higher than in non-iNPH patients, indicating that Tf glycan analysis by the high-throughput lectin-TfAb ELISAs could become practical diagnostic tools for iNPH. The lectin-antibody ELISAs of CSF proteins might be useful for diagnosis of the other neurological diseases.
Purification and characterization of FMRFamidelike immunoreactive substances from the lobster nervous system: Isolation and sequence analysis of two closely related peptides (1987)

In the preceding paper (Kobierski et al: J. Comp. Neurol. 266:1-15, '87) FMRFamidelike immunoreactivity (FLI) was localized to specific cells and processes in the nervous system of the lobster Homarus americanus. In an effort to establish a role for this material we have purified and characterized a variety of immunoreactive peptides that can be extracted from the secretory pericardial organs. By using gel-filtration chromatography and three different HPLC systems, it has been established that little or no authentic FMRFamide is present. Of the major immunoreactive components two peptides were purified in sufficient quantity for microsequence analysis and have been tentatively identified as the octapeptides Ser-Asp-Arg-Asn-Phe-Leu-Arg-Phe-amide (FLI 3) and Thr-Asn-Arg-Asn-Phe-Leu-Arg-Phe-amide (FLI 4). Both of these are novel neuropeptides with some sequence homology to the previously described FMRFamide family. The pericardial organs release FLI when depolarized with 100 mM K+ in the presence of calcium. Between 75 and 80% of this release is accounted for by FLI 3 and FLI 4. One of these peptides (FLI 4) has been synthesized and shown to cochromatograph with the endogenous immunoreactive material. Preliminary studies show that this peptide can act as a modulator of exoskeletal and cardiac neuromuscular junctions.
THE CHEMICAL COMPOSITION OF SOYA BEAN OIL (1922)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE CHEMICAL COMPOSITION OF SOYA BEAN OILWalter F. Baughman and George S. JamiesonCite this: J. Am. Chem. Soc. 1922, 44, 12, 2947–2952Publication Date (Print):December 1, 1922Publication History Published online1 May 2002Published inissue 1 December 1922https://pubs.acs.org/doi/10.1021/ja01433a035https://doi.org/10.1021/ja01433a035research-articleACS PublicationsRequest reuse permissionsArticle Views1358Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Copolymerization of styrene and acrylonitrile in aqueous dispersion (1956)

Abstract The bead copolymerization of styrene‐acrylonitrile, in the presence of 1‐azobis‐1‐phenylethane, was investigated at high temperature (90–100°C.). The initial distribution of acrylonitrile between styrene and the aqueous phase was determined and the change of this distribution during the copolymerization. The concentration of acrylonitrile in the aqueous phase remains fairly constant up to 20–25% conversion, then it decreases rapidly up to about 90% conversion. The over‐all rate of copolymerization following an induction period, remains constant up to about 30–35% conversion, then it increases slightly only to decrease rapidly at about 90% conversion. In a system of this type the mole fraction of acrylonitrile in the oil phase increases through the polymerization, partly because acrylonitrile reacts at a lower relative rate, partly because the acrylonitrile dissolved in the aqueous phase diffuses continuously into the beads. The reactivities of styrene and acrylonitrile in aqueous dispersion are the same as in bulk if the partition of acrylonitrile between the two phases is taken into account. The initial rates of copolymerization, in bulk, increase with the acrylonitrile content of the mixture. At high conversion, a marked acceleration in rates takes place. This autoacceleration is due to the increased viscosity of the medium and to the variation of the comonomer composition with conversion.
ChemInform Abstract: Negative‐Ion, Chemical‐Ionization Mass Spectrometry of Sulfonic Acid Esters of Carbohydrates. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The importance of radiation fog in the tropical seasonal rain forest of Xishuangbanna, south-west China (2008)

The tropical rain forest in Xishuangbanna, SW China has a high floristic diversity and is closely related to Malaysian rain forests in flora. This forest would not normally be established in such a climatic region as Xishuangbanna (less precipitation and lower air temperature) compared to those of the lowland moist tropics. The mean annual rainfall is 1487 mm, which is considerably lower than rain forests in other parts of the world. It is believed that the frequent occurrence of radiation fog might play an important role in the water relations of plants and in the hydrological cycle of this type of rain forest. However, the multiple hydrological and ecological effects of radiation fog are not well understood. In this paper, we describe and analyze the significance of radiation fog to this forest, and develop a hypothesis that fog plays an important role in the presence of the tropical rain forest in Xishuangbanna. Suggestions for further research on the significance of fog are offered.
The Physiological Significance of the Time-to-Maximum (Tmax) Parameter in Perfusion MRI (2010)

Special care should be taken when setting-up Tmax thresholds for data acquired with different protocols (eg, multicenter studies) because various factors can influence the measured Tmax. Because of its complementary information, used in conjunction with delay-insensitive cerebral blood-flow, cerebral blood volume, and mean transit time maps, Tmax should provide important additional information on brain hemodynamic status.
Enhanced hepatitis C virus genome replication and lipid accumulation mediated by inhibition of AMP-activated protein kinase (2010)

Hepatitis C virus (HCV) infection is associated with dysregulation of both lipid and glucose metabolism. As well as contributing to viral replication, these perturbations influence the pathogenesis associated with the virus, including steatosis, insulin resistance, and type 2 diabetes. AMP-activated protein kinase (AMPK) plays a key role in regulation of both lipid and glucose metabolism. We show here that, in cells either infected with HCV or harboring an HCV subgenomic replicon, phosphorylation of AMPK at threonine 172 and concomitant AMPK activity are dramatically reduced. We demonstrate that this effect is mediated by activation of the serine/threonine kinase, protein kinase B, which inhibits AMPK by phosphorylating serine 485. The physiological significance of this inhibition is demonstrated by the observation that pharmacological restoration of AMPK activity not only abrogates the lipid accumulation observed in virus-infected and subgenomic replicon-harboring cells but also efficiently inhibits viral replication. These data demonstrate that inhibition of AMPK is required for HCV replication and that the restoration of AMPK activity may present a target for much needed anti-HCV therapies.
Dynamic-range compression and contrast enhancement in infrared imaging systems (2008)

Third-generation thermal cameras have high dynamic range (up to 14 bits) and collect images that are difficult to visualize because their contrast exceeds the range of traditional display devices. Thus, sophisticated techniques are required to adapt the recorded signal to the display, maintaining, and possibly improving, objects' visibility and image contrast. The problem has already been studied in relation to images acquired in the visible spectral region, while it has been scarcely investigated in the infrared. In this work, this latter subject is addressed, and a new method is presented that combines dynamic-range compression and contrast enhancement techniques to improve the visualization of infrared images. The proposed method is designed to meet typical requirements in infrared sensor applications. The performance is studied through experimental data and compared with that yielded by three well-established algorithms. Evaluation is performed through subjective analysis, assigning each algorithm a score on the basis of the average opinion of human observers. The results demonstrate the effectiveness of the proposed technique in terms of perceptibility of details, edge sharpness, robustness against the horizon effect, and presence of very warm objects.
High-speed, area-efficient FPGA-based floating-point multiplier (2003)

In this paper, a floating-point multiplier with high speed and area efficient is presented. The multiplier is designed, optimized, and implemented on an FPGA based system. A comparison between the results of the proposed design and a previously reported one is provided. The effect of rounding on the area, speed, and accuracy for three different configurations is examined.
TBestDB: a taxonomically broad database of expressed sequence tags (ESTs) (2007)

The TBestDB database contains approximately 370,000 clustered expressed sequence tag (EST) sequences from 49 organisms, covering a taxonomically broad range of poorly studied, mainly unicellular eukaryotes, and includes experimental information, consensus sequences, gene annotations and metabolic pathway predictions. Most of these ESTs have been generated by the Protist EST Program, a collaboration among six Canadian research groups. EST sequences are read from trace files up to a minimum quality cut-off, vector and linker sequence is masked, and the ESTs are clustered using phrap. The resulting consensus sequences are automatically annotated by using the AutoFACT program. The datasets are automatically checked for clustering errors due to chimerism and potential cross-contamination between organisms, and suspect data are flagged in or removed from the database. Access to data deposited in TBestDB by individual users can be restricted to those users for a limited period. With this first report on TBestDB, we open the database to the research community for free processing, annotation, interspecies comparisons and GenBank submission of EST data generated in individual laboratories. For instructions on submission to TBestDB, contact tbestdb@bch.umontreal.ca. The database can be queried at http://tbestdb.bcm.umontreal.ca/.
Precision medicine in united airways disease: A “treatable traits” approach (2018)

United airways disease (UAD) is the concept that the upper and lower airways, which are anatomically and immunologically related, form a single organ. According to this concept, upper and lower airway diseases are frequently comorbid because they reflect manifestations of a single underlying disease at different sites of the respiratory tract. Allergic asthma-allergic rhinitis is the archetypal UAD, but emerging data indicate that UAD is a heterogeneous condition and consists of multiple phenotypes (observable clinical characteristics) and endotypes (pathobiologic mechanisms). The UAD paradigm also extends to myriad sinonasal diseases (eg, chronic rhinosinusitis with or without nasal polyps) and lower airway diseases (eg, bronchiectasis, chronic obstructive pulmonary disease). Here, we review currently known phenoendotypes of UAD and propose a "treatable traits" approach for the classification and management of UAD, wherein pathophysiological mechanisms and factors contributing to disease are identified and targeted for treatment. Treatable traits in UAD can be analyzed according to a framework comprising airway inflammation (eosinophilic, neutrophilic), impaired airway mucosal defense (impaired mucociliary clearance, antibody deficiency), and exogenous cofactors (allergic sensitizers, tobacco smoke, microbes). Appreciation of treatable traits is necessary in advancing the effort to deliver precise treatments and achieve better outcomes in patients with UAD.
Evidence that the substrate backbone conformation is critical to phosphorylation by p42 MAP kinase (2000)

The effect of prolyl bond isomers on the substrate recognition capabilities of various endoproteases may be investigated in a reaction where both cis/trans isomers co-exist. Here we address the question of whether enzyme reactions at the side chain of an amino acid preceding proline proceed through an isomer specific pathway. The proline-directed p42 mitogen-activated protein kinase (ERK2) was used to phosphorylate the serine side chain in Pro-Arg-Ser-Pro-Phe-4-nitroanilide under conditions where different amounts of cis prolyl isomer of the substrate were present. Initial phosphorylation rates were calculated ranging between zero at 100% cis isomer and around 60 pM/min at the equilibrium content of 83.5% trans isomer. In the presence of the peptidyl-prolyl cis/trans isomerase human hFKBP12 (500 nM), cis/trans isomerization proceeds rapidly, permitting the maximal phosphorylation rate to be observed in the dead time of the experiment. Results show that correct signature sequences are not sufficient to render potential substrates reactive to proline-directed enzymatic phosphorylations, but that the conformational state of the peptide bond following serine (threonine) is a critical determinant. Therefore, catalysis by peptidyl-prolyl cis/trans isomerases may add a new level of control to intracellular protein phosphorylations.