gebhart/patrepeval huggingface dataset:
Ultraviolet exposure of melanoma cells induces fibroblast activation protein-α in fibroblasts: Implications for melanoma invasion (2011)

Fibroblast activation protein-α (FAP-α) promotes tumor growth and cell invasiveness through extracellular matrix degradation. How ultraviolet radiation (UVR), the major risk factor for malignant melanoma, influences the expression of FAP-α is unknown. We examined the effect of UVR on FAP-α expression in melanocytes, keratinocytes and fibroblasts from the skin and in melanoma cells. UVR induces upregulation of FAP-α in fibroblasts, melanocytes and primary melanoma cells (PM) whereas keratinocytes and metastatic melanoma cells remained FAP-α negative. UVA and UVB stimulated FAP-α-driven migration and invasion in fibroblasts, melanocytes and PM. In co-culture systems UVR of melanocytes, PM and cells from regional metastases upregulated FAP-α in fibroblasts but only supernatants from non-irradiated PM were able to induce FAP-α in fibroblasts. Further, UV-radiated melanocytes and PM significantly increased FAP-α expression in fibroblasts through secretory crosstalk via Wnt5a, PDGF-BB and TGF-β1. Moreover, UV radiated melanocytes and PM increased collagen I invasion and migration of fibroblasts. The FAP-α/DPPIV inhibitor Gly-ProP(OPh)2 significantly decreased this response implicating FAP-α/DPPIV as an important protein complex in cell migration and invasion. These experiments suggest a functional association between UVR and FAP-α expression in fibroblasts, melanocytes and melanoma cells implicating that UVR of malignant melanoma converts fibroblasts into FAP-α expressing and ECM degrading fibroblasts thus facilitating invasion and migration. The secretory crosstalk between melanoma and tumor surrounding fibroblasts is mediated via PDGF-BB, TGF-β1 and Wnt5a and these factors should be evaluated as targets to reduce FAP-α activity and prevent early melanoma dissemination.
Improved complex demodulation method for frequency analysis (1991)

Abstract Sleep spindle brain waves have an important role in the sleeping mechanism. Recently, computer‐aided automatic analysis methods have been introduced in the form of numerical analysis of frequency, duration, etc., and their rapid progress has been noted. However, the spindle waves are transient nonstationary “phasic events,” and therefore their accurate frequency analysis has caused serious difficulties. Aiming at the accurate frequency analysis of the spindle waves, the authors have already proposed an analysis algorithm based on complex demodulation (CD) in the time domain, but the long processing time required for the analysis has remained a problem. This paper proposes a new analysis algorithm which combines the advantages of frequency domain CD and Hilbert transformation. This method applies Hilbert transformation mainly to speed up the measurement of instantaneous amplitude and, in the case of measuring the instantaneous frequency, the advantage of the CD method using interpolation is exploited to improve the accuracy. Here, the proposed method is explained concretely and the analysis results are investigated in comparison with the time domain CD method published earlier.
A high-fat, ketogenic diet induces a unique metabolic state in mice (2007)

Ketogenic diets have been used as an approach to weight loss on the basis of the theoretical advantage of a low-carbohydrate, high-fat diet. To evaluate the physiological and metabolic effects of such diets on weight we studied mice consuming a very-low-carbohydrate, ketogenic diet (KD). This diet had profound effects on energy balance and gene expression. C57BL/6 mice animals were fed one of four diets: KD; a commonly used obesogenic high-fat, high-sucrose diet (HF); 66% caloric restriction (CR); and control chow (C). Mice on KD ate the same calories as mice on C and HF, but weight dropped and stabilized at 85% initial weight, similar to CR. This was consistent with increased energy expenditure seen in animals fed KD vs. those on C and CR. Microarray analysis of liver showed a unique pattern of gene expression in KD, with increased expression of genes in fatty acid oxidation pathways and reduction in lipid synthesis pathways. Animals made obese on HF and transitioned to KD lost all excess body weight, improved glucose tolerance, and increased energy expenditure. Analysis of key genes showed similar changes as those seen in lean animals placed directly on KD. Additionally, AMP kinase activity was increased, with a corresponding decrease in ACC activity. These data indicate that KD induces a unique metabolic state congruous with weight loss.
RASID demo: A robust WLAN device-free passive motion detection system (2012)

WLAN device-free passive (DfP) indoor localization is an emerging technology enabling the localization of entities that neither carry any devices nor participate actively in the localization process using the already installed wireless infrastructure. This technology can be promising for a variety of applications, where special hardware might not be applicable such as intrusion detection and smart homes. We present a demonstration of RASID, a system capable of detecting passive human motion using the already installed wireless networks. RASID uses statistical anomaly detection techniques to detect motion inside indoor environments. The system also is capable of adapting to the environment changes for enhancing its accuracy.
Purification, partial sequences and properties of chicken insulin-like growth factors (1988)

Insulin-like growth factor-I (IGF-I) and IGF-II have been purified to homogeneity from chicken serum as a step towards the characterization of the roles for these peptides in the growth process. Chicken IGF-I had about half the efficacy of bovine/human IGF-I in a bioassay and in radioimmunoassays with bovine IGF-I as radioligand. Chicken IGF-II competed for the binding of bovine IGF-II to cell receptors while chicken IGF-I reacted minimally in this IGF-II radioreceptor assay. Further evidence of homology was obtained by N-terminal sequence analysis of the first 31 and 35 amino acids of chicken IGF-I and IGF-II respectively. Chicken IGF-I had the same N-terminal as human IGF-I, with the exception of the substitution of serine for asparagine at residue 26. Chicken IGF-II had a unique N-terminal tetrapeptide Tyr-Gly-Thr-Ala, but from residues 5-30 the sequence was identical to that reported for residues 6-31 of human IGF-II. Substitutions also occurred corresponding to residues 32, 33, 35 and 36 of human IGF-II. A variant form of chicken IGF-II that had the same N-terminal pentapeptide as human IGF-II was also detected.
Inhibition by prostaglandins of leukotriene B4 release from activated neutrophils. (1983)

Chemoattractant N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) in the presence of cytochalasin B stimulates the release of leukotriene B4 (LTB4), superoxide (O2-), and N-acetylglucosaminidase from elicited rat peritoneal and human peripheral neutrophils [PMN (polymorphonuclear leukocytes)]. Prostaglandins E1 and E2 (PGE1 and PGE2) inhibit LTB4 release from PMN in a dose-related manner with an IC50 of 1 X 10(-8) M. This action is associated with increased levels of cyclic AMP. The inhibitory activity of a variety of PGs on LTB4 production by rat peritoneal PMN parallels their affinity for PGE receptors in other tissues. O2- release is also suppressed by low levels of PGE1 and PGE2 in a dose-related manner and this inhibition is enhanced by theophylline. In contrast, lysosomal enzyme release is only minimally affected by physiological levels of PGs. These data are consistent with an action of PGs at the level of the PG receptor on LTB4 and O2- release from the fMet-Leu-Phe-stimulated rat peritoneal PMN. In addition, the fMet-Leu-Phe-induced adherence of PMN to endothelial cells and inhibition of this phenomenon by PGs may now be explained by PG-mediated inhibition of LTB4 formation.
Extraordinary Aggregation of Inorganic Anions in Chiral Metallosupramolecular Ionic Crystals (2013)

Abstract An optically active AuI4CoIII2 complex with mixed d-penicillaminate (d-pen) and 1,2-bis(diphenylphosphino)ethane (dppe), [Au4Co2(dppe)2(d-pen)4]2+, was selectively formed from [Au2(dppe)(d-pen)2]2− and CoIII. When this complex was crystallized with monovalent anions (ClO4−, BF4−, NO3−, Cl−, Br−, and N3−), the AuI4CoIII2 complex cations were self-assembled to form +12-charged supramolecular octahedrons that are closely packed in a face-centered cubic (fcc) structure. In this structure, ten monovalent anions are accommodated in each hydrophilic tetrahedral interstice to create an adamantane-shaped anionic cluster, while two anions are each encapsulated in each octahedral interstice and in the center of each supramolecular octahedron. The use of divalent anions (SO42− and SiF62−) also produced an analogous fcc structure made up of the +12-charged supramolecular octahedrons. In this case, six divalent anions are accommodated in each hydrophilic tetrahedral interstice to form an octahedron-shaped anionic cluster, completing a giant zinc blende lattice structure where all cations and anions are separately aggregated into the +12-charged octahedrons and the −12-charged octahedrons, respectively. The site selective aggregation of two kinds of inorganic anions (PF6−/Br−, BF4−/Br−, and NO3−/Br−) in the fcc structure, along with the synthesis and characterization of an analogous AuI4CrIII2 complex, is also reported.
Analysis of Cell-Specific Promoters for Viral Gene Therapy Targeted at the Vascular Endothelium (2001)

The use of viral vectors for vascular gene therapy targeted at the endothelium is limited by the promiscuous tropism of vectors and nonspecificity of viral promoters, resulting in high-level transgene expression in multiple tissues. To evaluate suitable endothelial cell (EC)-specific promoters for vascular gene therapy, we directly compared the ability of the fms-like tyrosine kinase-1 (FLT-1), intercellular adhesion molecule-2 (ICAM-2), and von Willebrand factor (vWF) promoters to drive EC-restricted transcription after cloning into adenoviral vectors upstream of lacZ. Vastly different expression profiles were observed. Whereas both FLT-1 and ICAM-2 promoters generated transgene expression levels similar to cytomegalovirus in ECs in vitro, vWF expression levels were extremely low. Analysis of non-EC types revealed that ICAM-2 but not FLT-1 evoked leaky transgene expression, thus identifying FLT-1 as the most selective promoter. With an ex vivo human gene therapy model, the FLT-1 promoter demonstrated EC-specific transgene expression in intact human vein but no detectable expression from infected exposed smooth muscle cells in EC-denuded vein. Furthermore, when adenoviruses were systemically administered to mice, the FLT-1 promoter demonstrated extremely low-level gene expression in the liver, the major target organ for adenoviral transduction in vivo. This study highlights the potential of using the FLT-1 promoter for local and systemic human gene therapy in hypertension and its complications.
Bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patients (2008)

The intestinal microbiota of CD patients could contribute to the Th1 pro-inflammatory milieu characteristic of the disease, while B. longum ES1 and B. bifidum ES2 could reverse these deleterious effects. These findings hold future perspectives of interest in CD therapy.
Optical Bandgaps of π‐Conjugated Organic Materials at the Polymer Limit: Experiment and Theory (2007)

Abstract In this Review, the extreme care that must be taken when predicting the optical properties of conjugated polymers via the oligomer approach, and when comparing theoretical and experimental data, is illustrated. In the first part, conceptual strategies for the correct determination of optical transitions from experimental spectra and relevant extrapolation procedures at the polymer limit are introduced. The impact of conformational, substitution, solvent, and solid‐state effects on the optical properties is discussed in light of experimental data reported for molecular backbones based on phenylene, phenylenevinylene, and thiophene repeat units. A comparison is then made between experimental results and those provided by standard quantum‐chemical methods, to assess their reliability.
Lipooligosaccharide Structure Contributes to Multiple Steps in the Virulence of<i>Neisseria meningitidis</i> (2006)

Lipooligosaccharide (LOS) of Neisseria meningitidis has been implicated in meningococcal interaction with host epithelial cells and is a major factor contributing to the human proinflammatory response to meningococci. LOS mutants of the encapsulated N. meningitidis serogroup B strain NMB were used to further determine the importance of the LOS structure in in vitro adherence and invasion of human pharyngeal epithelial cells by meningococci and to study pathogenicity in a mouse (CD46 transgenic) model of meningococcal disease. The wild-type strain [NeuNAc-Galbeta-GlcNAc-Galbeta-Glcbeta-Hep2 (GlcNAc, Glcalpha) 3-deoxy-D-manno-2-octulosonic acid (KDO2)-lipid A; 1,4' bisphosphorylated], although poorly adherent, rapidly invaded an epithelial cell layer in vitro, survived and multiplied early in blood, reached the cerebrospinal fluid, and caused lethal disease in the mouse model. In contrast, the Hep2 (GlcNAc) KDO2-lipid A (pgm) mutant, which was highly adherent to cultured epithelial cells, caused significantly less bacteremia and mortality in the mouse model. The Hep2-KDO2-lipid A (rfaK) mutant was shown to be moderately adherent and to cause levels of bacteremia and mortality similar to those caused by the wild-type strain in the mouse model. The KDO2-lipid A (gmhB) mutant, which lacks the heptose disaccharide in the inner core of LOS, avidly attached to epithelial cells but was otherwise avirulent. Disease development correlated with expression of specific LOS structures and was associated with lower adherence but rapid meningococcal passage to and survival in the bloodstream, induction of proinflammatory cytokines, and the crossing of the blood-brain barrier. Taken together, the results of this study further define the importance of the LOS structure as a virulence component involved in multiple steps in the pathogenesis of N. meningitidis.
High efficiency separation of microbial aggregates using capillary electrophoresis (2000)

Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1000000 plates m(-1) in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible with brief immersion in an ultrasound bath. This reversibility was confirmed by visual microscopy and electrophoretic data.
Monitoring mandibular movements to detect Cheyne-Stokes Breathing (2017)

CSB in patients with sleep disordered breathing could be accurately identified by a simple magnetometer device recording mandibular movements.
α‐substituierte Phosphonate. 47. PC‐Spaltungen bei geminalen Trisphosphorylverbindungen (1986)

α‐Substituted Phosphonates. 47. Splitting of PC‐Bonds from Trisphosphoryl Compounds Hexaethyldimethylaminomethyl‐trisphosphonate ( 2a ) reacts with HCl or trimethylsilylbromid/water by splitting off one phosphoryl group, giving dimethylaminomethyl‐bisphosphonic acid ( 7 ). The result of methylation of 2a depends on the reagent: with methyliodide one phosphoryl group is split off, and the ammonium salt 11a is formed, while with methyl toluene sulfonate or dimethylsulfate the expected quarternary ammonium salts 10b or 10c are formed. One phosphoryl group of 10c is split off under acidic as well as alkalinous conditions: acidic hydrolysis gives the bisphosphonic acid 15 , while under alkalinous conditions the known ylid 16 is formed.
Cabozantinib Loaded DSPE-PEG2000 Micelles as Delivery System: Formulation, Characterization and Cytotoxicity Evaluation (2015)

Cabozantinib, a potent pan-tyrosine kinase inhibitor, has been reported to provide enhanced antitumor efficacy by simultaneously inhibiting both MET and VEGF pathways, which are critical to tumor angiogenesis, survival and migration. It's very poor water solubility prevents its administration by the intravenous route, which may be important in patients unable to take the drug orally. In this study, we developed an efficient PEG-lipid-based polymeric micelle formulation with enhanced drug solubility and stability for cabozantinib delivery. DSPE-PEG<sub>2000</sub> micelles encapsulating cabozantinib were prepared by a thin-film rehydration method followed by a lyophilization process to generate the dry dosage form. The average hydrodynamic diameter of freshly prepared micelles was 11 nm with a narrow size distribution, and the dry micelle cake could be fully reconstituted by rehydration. Approximately 75% of the drug was encapsulated into the lyophilized cake, and a sustained drug release profile was observed in simulated normal physiological release medium. Compared with the free cabozantinib solution, the drug-loaded micelles displayed significantly enhanced intracellular accumulation and cytotoxicity in human glioblastoma cancer cells and non-small lung cancer cells. These results suggest that the micellar formulation of cabozantinib may serve as a promising nanocarrier in anticancer treatments.
Alpha7 nicotinic acetylcholine receptors and neural network synaptic transmission in human induced pluripotent stem cell-derived neurons (2019)

The α7 nicotinic acetylcholine receptor has been extensively researched as a target for treatment of cognitive impairment in Alzheimer's disease and schizophrenia. Investigation of the α7 receptor is commonly performed in animals but it is critical to increase the biologically relevance of the model systems to fully capture the physiological role of the α7 receptor in humans. For example most humans, in contrast to animals, express the hybrid gene CHRFAM7A, the product of which modulates α7 receptor activity. In the present study, we used human induced pluripotent stem cell (hiPSC) derived neurons to establish a humanized α7 model. We established a cryobank of neural stem cells (NSCs) that could reproducibly be matured into neurons expressing neuronal markers and CHRNA7 and CHRFAM7A. The neurons responded to NMDA, GABA, and acetylcholine and exhibited synchronized spontaneous calcium oscillations. Gene expression studies and application of a range of α7 positive allosteric modulators (PNU-120595, TQS, JNJ-39393406 and AF58801) together with the α7 agonist PNU-282987 during measurement of intracellular calcium levels demonstrated the presence of functional α7 receptors in matured hiPSC-derived neuronal cultures. Pharmacological α7 activation also resulted in intracellular signaling as measured by ERK 1/2 phosphorylation and c-Fos protein expression. Moreover, PNU-120596 increased the frequency of the spontaneous calcium oscillations demonstrating implication of α7 receptors in human synaptic networks activity. Overall, we show that hiPSC derived neurons are an advanced in vitro model for studying human α7 receptor pharmacology and the involvement of this receptor in cellular processes as intracellular signaling and synaptic transmission.
Synchrony of Clonal Cell Proliferation and Contiguity of Clonally Related Cells: Production of Mosaicism in the Ventricular Zone of Developing Mouse Neocortex (1997)

We have analyzed clonal cell proliferation in the ventricular zone (VZ) of the early developing mouse neocortex with a replication-incompetent retrovirus encoding human placental alkaline phosphatase (AP). The retrovirus was injected into the lateral ventricles on embryonic day 11 (E11), i.e., at the onset of neuronogenesis. Three days postinjection, on E14, a total of 259 AP-labeled clones of various sizes were found in 7 fetal brains. There are approximately 7 cell cycles between E11 and E14 (), and there is a 1-2 cell cycle delay between retroviral injection and the production of a retrovirally labeled "founder" cell; thus, we estimate that the "age" of the clones was about 5-6 cell cycles. Almost one-half of the clones (48.3%) identified were pure proliferating clones containing cells only in the VZ. Another 18.5% contained both proliferating and postproliferative cells, and 33.2% contained only postproliferative cells. It was striking that over 90% of the clonally related proliferating cells occurred in clusters of two or more apparently contiguous cells, and about 73% of the proliferating cells occurred in clusters of three or more cells. Regardless of the number of cells in the clone, these clusters were tightly packed and confined to a single level of the VZ. This clustering of proliferating cells indicates that clonally related cells maintain neighbor-neighbor relationships as they undergo interkinetic nuclear migration and progress through several cell cycles, and, as a result, the ventricular zone is a mosaic of small clusters of clonally related and synchronously cycling cells. In addition, cells in the intermediate zone and the cortical plate were also frequently clustered, indicating that they became postproliferative at a similar time and that the output of the VZ is influenced by its mosaic structure.
Joint operational decision-making in collaborative transportation networks: the role of IT (2014)

Purpose – This paper addresses horizontal supply chain collaboration among autonomous freight carriers in the less-than-truckload industry. The main purpose of the paper is to identify and explain the challenges with joint operational decision-making in this context and investigate the precise role of information technology (IT) therein. Design/methodology/approach – Empirical evidence is gathered by means of exploratory and explanatory case research, with multiple cases at the planning departments of European freight carriers operating in collaborative transportation networks. Findings – Collaborating carriers face fundamental challenges in joint operational planning and control of collaborative transportation, despite the broad availability of state-of-the-art IT. These operational supply chain challenges can be explained by technological differences of the available IT applications, which hinder integration. Research limitations/implications – Any expectations with regard to state-of-the-art XML or EDI-based IT integration for improved joint operational decision-making in collaborative transportation networks should be considered with care. In particular, the authors' research findings may encourage practitioners to consider new planning and control procedures and develop dedicated IT applications for collaborating freight carriers. Originality/value – The paper highlights the importance of horizontal supply chain collaboration for small and medium-sized freight carriers in the less-than-truckload industry and conceptualises how collaborative transportation networks are organised. Furthermore, an IT typology is introduced to explain the challenges with joint operational decision-making.