gebhart/patrepeval huggingface dataset:
Prognostic significance of <i>K-Ras</i> mutation rate in metastatic colorectal cancer patients (2015)

Our data demonstrate an association between K-Ras mutation rate and prognosis in mCRC patients treated with bevacizumab-containing first-line therapy. These data deserve to be verified in an independent validation set.
3-Hydroxy-2-naphthoates of lidocaine, mepivacaine, and bupivacaine and their dissolution characteristics. (1978)

3-Hydroxy-2-naphthoates of lidocaine (I), mepivacine (II), and bupivacaine (III) were prepared as a potential means of prolonging the action of these local anesthetic amines through their sparingly soluble salt forms. The molar ratio of the local anesthetic base to 3-hydroxy-2-naphthoic acid (IV) was 1 : 1 and the hydroxynaphthoates contained no solvent of crystallization. Dissolution characteristics of these hydroxynaphthoates were determined in water and 0.7M phosphate buffer, pH 7.46 at 37°and compared with those of the corresponding bases. The hydroxynaphthoates of I and II exhibited lower equilibrium solubilities but their dissolution rates were greater than the corresponding bases in these media. As for III, the hydroxynaphthoate showed higher solubility than the base in water.
Interactive Mission Planning System of an Autonomous Vehicle Fleet that Executes Services (2020)

Automated solutions for executing services in urban areas have become a trend in the past years. Examples of such services are: package delivery, transportation, street cleaning, waste disposal or vegetation care. They are also part of new concepts of smart cities. The vision is to have a vehicle fleet that provides these services at demand of inhabitants or authorities in urban areas. These vehicles have the capability to drive autonomously and interact with other traffic participants in order to accomplish a specific task. An important aspect is the mission planning of the vehicles. We present a concept of an interactive planning and management of a vehicle fleet that executes requested service demands in urban areas and the interaction between the involved stakeholders. The service requester gets immediate response regarding their request and can track, change or cancel it with immediate adaption of the plan. The concept was implemented and the system was used a few months for delivery services during a real world laboratory in a new built district in Heilbronn (Germany), with about 800 inhabitants.
HR gets its house in order : on the cover (2012)

This framework for the National HR Competency Model sets the benchmark for HR professionalism.
<i>Agrobacterium</i>-Mediated Transformation of <i>Fusarium oxysporum</i>: An Efficient Tool for Insertional Mutagenesis and Gene Transfer (2001)

ABSTRACT Agrobacterium tumefaciens-mediated transformation (ATMT) has long been used to transfer genes to a wide variety of plants and has also served as an efficient tool for insertional mutagenesis. In this paper, we report the construction of four novel binary vectors for fungal transformation and the optimization of an ATMT protocol for insertional mutagenesis, which permits an efficient genetic manipulation of Fusarium oxysporum and other phytopathogenic fungi to be achieved. Employing the binary vectors, carrying the bacterial hygromycin B phosphotrans-ferase gene (hph) under the control of the Aspergillus nidulans trpC promoter as a selectable marker, led to the production of 300 to 500 hygromycin B resistant transformants per 1 x 10(6) conidia of F. oxysporum, which is at least an order of magnitude higher than that previously accomplished. Transformation efficiency correlated strongly with the duration of cocultivation of fungal spores with Agrobacterium tumefaciens cells and significantly with the number of Agrobacteruium tumefaciens cells present during the cocultivation period (r = 0.996; n = 3; P < 0.01). All transformants tested remained mitotically stable, maintaining their hygromycin B resistance. Growing Agrobacterium tumefaciens cells in the presence of acetosyringone (AS) prior to cocultivation shortened the time required for the formation of transformants but decreased to 53% the percentage of transformants containing a single T-DNA insert per genome. This increased to over 80% when Agrobacterium tumefaciens cells grown in the absence of AS were used. There was no correlation between the average copy number of T-DNA per genome and the colony diameter of the transformants, the period of cocultivation or the quantity of Agrobacterium tumefaciens cells present during cocultivation. To isolate the host sequences flanking the inserted T-DNA, we employed a modified thermal asymmetric interlaced PCR (TAIL-PCR) technique. Utilizing just one arbitrary primer resulted in the successful amplification of desired products in 90% of those transformants analyzed. The insertion event appeared to be a random process with truncation of the inserted T-DNA, ranging from 1 to 14 bp in size, occurring on both the right and left border sequences. Considering the size and design of the vectors described here, coupled with the efficiency and flexibility of this ATMT protocol, it is suggested that ATMT should be regarded as a highly efficient alternative to other DNA transfer procedures in characterizing those genes important for the pathogenicity of F. oxysporum and potentially those of other fungal pathogens.
An evaluation of dynamic SPECT imaging methods (2002)

Techniques to estimate dynamic parameters from data acquired in a single rotation of a single head SPECT camera are being investigated. We have tested the performance of various reconstruction methods. The linear least squares (LLS) method uses a simple assumption that the activity within each object pixel decreases over the imaging time. In simulations, we have used an analytical heart model with single and biexponential radiopharmaceutical decay, with and without background activity and noise. Studies using a single and a 90/spl deg/ dual head camera were modelled with a total acquisition time of 20 min, performing rotation over 180/spl deg/. These simulations were followed by experimental scans of our dynamic phantom used with and without attenuation. Dynamic images from simulations and phantom experiments reconstruct well. The accuracy of reconstructed half-lives for the most challenging case of the single head camera remains within 10% for simulated data, and 20% for the phantom data. Some streaking artifacts which are evident in images corresponding to early reconstruction times subside upon incorporating constraints and regularizations.
Analysis of Negotiation Support System Research (1995)

(1995). Analysis of Negotiation Support System Research. Journal of Computer Information Systems: Vol. 35, No. 3, pp. 2-11.
Buffer minimization of real-time streaming applications scheduling on hybrid CPU/FPGA architectures (2009)

We address the problem of real-time streaming applications scheduling on hybrid CPU/FPGA architectures. The main contribution is a two-step approach to minimize the buffer requirement for streaming applications with throughput guarantees. A novel declarative way of constraint based scheduling for real-time hybrid SW/HW systems is proposed, while the application throughput is guaranteed by periodic phases in execution. We use a voice-band modem application to exemplify the scheduling capabilities of our method. The experimental results show the advantages of our techniques in both less buffer requirement and higher throughput guarantees compared to the traditional PAPS method.
Beryllium foil transition-radiation source for x-ray lithography (1991)

We have measured the total soft-x-ray power from a transition radiator composed of a stack of 25 beryllium foils each 1.0 μm thick which were penetrated by a relativistic electron beam whose maximum power was approximately 7 kW. The maximum total soft-x-ray power was measured to be 15.2 mW for a 245 MeV, 37 μA electron beam. The bandwith of the radiation at the full width half maximum points was calculated to be between 0.6 and 1.6 keV. In addition, we have exposed photoresist-coated silicon wafers at a distance of 3 m from the radiator. Exposure times of the bare resist were as short as 120 s for 5 cm2 of wafer are (resist sensitivity is 55.6 mJ/cm2). The shortest time for mask/wafer exposure was 180 s for 5 cm2.
A 10 bit 20 MS/s 3 V supply CMOS A/D converter (1994)

A 10 bit CMOS A/D converter with 3 V power supply has been developed for being integrated into system VLSI's. In this A/D converter, redundant binary encoders named "twin encoders" enhance tolerance to substrate noise, together with employing differential amplifiers in comparators. The bias circuit using a replica of the amplifier is developed for biasing differential comparators with 3 V power supply. Subranging architecture along with a multilevel tree decoding structure improves dynamic performance of the ADC at 3 V power supply. The A/D converter is fabricated in double-polysilicon, double-metal, 0.8 /spl mu/m CMOS technology. The experimental results show that the ADC operates at 20 MS/s and the twin encoders suppress the influence of substrate noise effectively. This ADC has a single power supply of 3 V, and dissipates 135 mW at 20 MS/s operation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
DNA Methylation Map of Human Atherosclerosis (2014)

Our data identify an atherosclerosis-specific DNA methylation profile that highlights the contribution of different genes and pathways to the disorder. Interestingly, the observed gain of DNA methylation in the atherosclerotic lesions justifies efforts to develop DNA demethylating agents for therapeutic benefit.
Rapid evaluation of gonococcal and nongonococcal urethritis in men with Limulus amoebocyte lysate and a chromogenic substrate (1983)

A chromogenic substrate was used with Limulus amoebocyte lysate (LAL) and compared by parallel testing with the traditional gelation LAL method for the rapid evaluation of exudative urethritis in 125 male patients. Of these patients, 67 had positive cultures for Neisseria gonorrhoeae and 58 were negative. The corresponding prevalence of gonococcal urethritis was 53.6%. For assay, diluted urethral samples and chromogenic substrate were added directly to single-test LAL vials, and objective color endpoint determinations were made visually after a 10-min incubation period at 37 degrees C. Sensitivity and specificity were 98.5% and 93.1%, respectively, with an overall accuracy in predicting culture results of 96.0%. The predictive value of a positive LAL test was 94.3% in our patient population; in a population with a prevalence of gonococcal urethritis of only 10%, the predictive value would be 61.3%. Results were not statistically different from those obtained by the 30-min gelation LAL method or by Gram-stained smears read by experienced microscopists (P greater than 0.05). Unlike the delicate gel, the color endpoint was not prone to accidental mechanical disruption during incubation or reading. Thus, use of a chromogenic substrate greatly improved the utility and speed of the LAL assay for evaluating men with exudative urethritis while not affecting the accuracy of the test.
HASBE: A Hierarchical Attribute-Based Solution for Flexible and Scalable Access Control in Cloud Computing (2011)

Cloud computing has emerged as one of the most influential paradigms in the IT industry in recent years. Since this new computing technology requires users to entrust their valuable data to cloud providers, there have been increasing security and privacy concerns on outsourced data. Several schemes employing attribute-based encryption (ABE) have been proposed for access control of outsourced data in cloud computing; however, most of them suffer from inflexibility in implementing complex access control policies. In order to realize scalable, flexible, and fine-grained access control of outsourced data in cloud computing, in this paper, we propose hierarchical attribute-set-based encryption (HASBE) by extending ciphertext-policy attribute-set-based encryption (ASBE) with a hierarchical structure of users. The proposed scheme not only achieves scalability due to its hierarchical structure, but also inherits flexibility and fine-grained access control in supporting compound attributes of ASBE. In addition, HASBE employs multiple value assignments for access expiration time to deal with user revocation more efficiently than existing schemes. We formally prove the security of HASBE based on security of the ciphertext-policy attribute-based encryption (CP-ABE) scheme by Bethencourt <etal xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"/> and analyze its performance and computational complexity. We implement our scheme and show that it is both efficient and flexible in dealing with access control for outsourced data in cloud computing with comprehensive experiments.
Mechanism of action of cutinase: chemical modification of the catalytic triad characteristic for serine hydrolases (1982)

Cutinase from Fusarium solani f. sp. pisi was inhibited by diisopropyl fluorophosphate and phenylboronic acid, indicating the involvement of an active serine residue in enzyme catalysis. Quantitation of the number of phosphorylated serines showed that modification of one residue resulted in complete loss of enzyme activity. One essential histidine residue was modified with diethyl pyrocarbonate. This residue was buried in native cutinase and became accessible to chemical modification only after unfolding of the enzyme by sodium dodecyl sulfate. The modification of carboxyl groups with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide in the absence of sodium dodecyl sulfate did not result in inactivation of the enzyme; however, such modifications in the presence of sodium dodecyl sulfate resulted in complete loss of enzyme activity. The number of residues modified was determined by incorporation of [14C]glycine ethyl ester. Modification of cutinase in the absence of sodium dodecyl sulfate and subsequent unfolding of the enzyme with detergent in the presence of radioactive glycine ester showed that one buried carboxyl group per molecule of cutinase resulted in complete inactivation of the enzyme. Three additional peripheral carboxyl groups were modified in the presence of sodium dodecyl sulfate. Carbethoxylation of the essential histidine and subsequent incubation with the esterase substrate p-nitrophenyl [1-14C]acetate revealed that carbethoxycutinase was about 10(5) times less active than the untreated enzyme. The acyl-enzyme intermediate was stabilized under these conditions and was isolated by gel permeation chromatography. The results of the present chemical modification study indicate that catalysis by cutinase involves the catalytic triad and an acyl-enzyme intermediate, both characteristic for serine proteases.
Differential Substrate Specificity and Kinetic Behavior of <i>Escherichia coli</i> YfdW and <i>Oxalobacter formigenes</i> Formyl Coenzyme A Transferase (2008)

The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic behavior remain to be elucidated, findings from structural genomic studies have shown that the structure of YfdW, the protein encoded by the yfdW gene, is homologous to that of the enzyme that mediates oxalate catabolism in the obligate anaerobe Oxalobacter formigenes, O. formigenes formyl coenzyme A transferase (FRC). We now report the first detailed examination of the steady-state kinetic behavior and substrate specificity of recombinant, wild-type YfdW. Our studies confirm that YfdW is a formyl coenzyme A (formyl-CoA) transferase, and YfdW appears to be more stringent than the corresponding enzyme (FRC) in Oxalobacter in employing formyl-CoA and oxalate as substrates. We also report the effects of replacing Trp-48 in the FRC active site with the glutamine residue that occupies an equivalent position in the E. coli protein. The results of these experiments show that Trp-48 precludes oxalate binding to a site that mediates substrate inhibition for YfdW. In addition, the replacement of Trp-48 by Gln-48 yields an FRC variant for which oxalate-dependent substrate inhibition is modified to resemble that seen for YfdW. Our findings illustrate the utility of structural homology in assigning enzyme function and raise the question of whether oxalate catabolism takes place in E. coli upon the up-regulation of the yfdXWUVE operon under acidic conditions.
[Ultrasonographic value for the complications of breast augmentation with injectable polyacrylamide hydrogel technique]. (2007)

Ultrasonography may be of great diagnostic value for the complications of breast augmentation with polyamide hydrogel injection.
The Preparation of Finely Divided Metal and Alloy Powders (1990)

Finely divided metal and alloy powders can be prepared by reduction or co‐reduction of metal salts in organic solvents. By means of this novel “cold powder technology”, microcrystalline to amorphous metals and alloys are obtained (particle size according to SEM photographs 10–100 nm) which can also be generated on support materials. This is of interest for catalytic applications.
Effects of capsaicin desensitization on taste in humans (1995)

The desensitization effects on taste resulting from application of 100 or 10 ppm capsaicin, accompanied by daily testing of a capsaicin series (1-1000 ppm, in log steps), or the desensitization resulting from application of 100 ppm capsaicin without the daily capsaicin testing, were investigated. The taste stimuli were three concentrations each of NaCl, sucrose, citric acid, quinine and 6-n-propylthiouracil. Following either type of 100 ppm desensitization, the magnitude estimates of the two bitter tastes, in particular, and citric acid showed significant decrements. Following 10 ppm capsaicin or an ethanol control procedure, there were no such effects. Recovery was complete in 1-3 days. It seems possible that the taste decrements are due to effects on both the taste and tactile components of taste, though there is a stronger case for effects on the tactile component.
Clinical importance of lactic acid bacteria: a short review. (2011)

Lactic acid bacteria (LAB) were used extensively as starter cultures in food fermentation. Some of the health benefits which have been claimed for lactic acid bacteria as probiotics include the following: improvement of the normal microflora, prevention of infectious diseases and food allergies, reduction of serum cholesterol, anticarcinogenic activity, stabilization of the gut mucosal barrier, immune adjuvant properties, alleviation of intestinal bowel disease symptoms and improvement in the digestion of lactose in intolerant hosts. The present study is aimed to brief review the some clinical importance of lactic acid bacteria (www.actabiomedica.it).
A permanent borehole fiber-optic distributed acoustic sensing experiment (2013)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 2013A permanent borehole fiber-optic distributed acoustic sensing experimentAuthors: Q. LiB. HornbyJ. KonklerQ. LiBP, B. HornbyBP, and J. KonklerBPhttps://doi.org/10.1190/segam2013-0698.1 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Abstract We show an initial analysis of data from a permanent borehole fiber-optic Distributed Acoustic Sensing (DAS) and dedicated receiver experiment in Milne Point, Alaska. Results detail direct comparison of data from both fiberoptic DAS receivers and the z-component of actual geophones, all permanently cemented in a borehole, with a surface vibroseis source. We examine data amplitude, frequency, phase, noise and other practical issues. We also present quantitative comparisons of signal/noise for both dedicated and virtual DAS receivers as well as some thoughts on the way forward for the DAS technology. Permalink: https://doi.org/10.1190/segam2013-0698.1FiguresReferencesRelatedDetailsCited byUnpaired training: Optimize the seismic data denoising model without paired training dataHaitao Ma, He Ba, Yue Li, Yuxing Zhao, and Ning Wu16 January 2023 | GEOPHYSICS, Vol. 88, No. 1DAS noise attenuation using wavelet stackZhou Yu, Zhaojun Liu, Ge Zhan, Peng Cheng, Bin Wang, and Weihong Fei15 August 2022Downhole Monitoring Using Distributed Acoustic Sensing: Fundamentals and Two Decades Deployment in Oil and Gas Industries21 March 2022A Literature Review10 December 2021Achieving business value using high-end borehole geophysics: Are we there yet?Brian Hornby17 August 2017Special Session I Complete Session17 August 2017Valhall dual-well 3D DAS VSP field trial and imaging for active wellsTao Jiang, Ge Zhan, Thomas Hance, Setiawardono Sugianto, Sebastien Soulas, and Einar Kjos1 September 2016Vertical Seismic Profile Complete Session1 September 2016VSP field trials of distributed acoustic sensing in Trinidad and Gulf of MexicoGe Zhan*, Jan Kommedal, and Jay Nahm19 August 2015Dual-well 3D vertical seismic profile enabled by distributed acoustic sensing in deepwater Gulf of MexicoHan Wu, Wai-Fan Wong, Zhaohui Yang, Peter B. Wills, Jorge L. Lopez, Yingping Li, Bastian Blonk, Ben Hewett, and Albena Mateeva27 July 2015 | Interpretation, Vol. 3, No. 3Field testing a three-component fiber-optic borehole seismic sensor arrayMartin Karrenbach*, Victor Yartsev, Melissa Emuh, Ekaterina Hardin, Lisa LaFlame, Steve Cole, and Andres Chavarria5 August 2014 SEG Technical Program Expanded Abstracts 2013ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 2013 Pages: 5258 Publisher:Society of Exploration Geophysicists HistoryPublished Online: 19 Aug 2013 CITATION INFORMATION Q. Li, B. Hornby, and J. Konkler, (2013), "A permanent borehole fiber-optic distributed acoustic sensing experiment," SEG Technical Program Expanded Abstracts : 5057-5061. https://doi.org/10.1190/segam2013-0698.1 Plain-Language Summary PDF DownloadLoading ...
STREPTOMYCIN USED IN THE TREATMENT OF SUPPURATIVE OTITIS MEDIA: A Preliminary Report (1947)

A MULTITUDE of measures have been tried in the treatment of suppurative otitis media. None is entirely satisfactory. This is particularly true in cases in which the otitis is chronic. A large percentage of the ears in which the condition is refractive will be found to be infected with gram-negative bacilli, namely, Bacillus proteus and Pseudomonas aeruginosa. In ears from which these organisms were cultured, the results obtained with the usual methods of treatment in our hands, including the sulfonamide drugs and penicillin, have been consistently disappointing, though these same organisms are supposedly saprophytic. Often they must be pathogens. Since gram-negative bacilli usually are resistant to sulfonamide compounds and penicillin in vitro and in vivo, streptomycin was tried in a small series of ears infected with gram-negative bacilli after their sensitivity to this antibiotic had been proved in the laboratory by other workers. Streptomycin was used in a series of
Routine Use of Intraoperative Neuromonitoring During ACDFs for the Treatment of Spondylotic Myelopathy and Radiculopathy Is Questionable (2016)

3.
Interleukin 2 production in whole blood culture: a rapid test of immunity to Francisella tularensis (1985)

The measurement of interleukin 2 from antigen-stimulated whole blood culture supernatants was used to detect cell-mediated immunity in subjects sensitized against Francisella tularensis. The amount of interleukin 2 produced differentiated a positive immune reaction sensitively and reliably in a 24-h culture. Whole blood culturing is an easy method for producing interleukin 2.
Methylene blue used in the treatment of refractory shock resulting from drug poisoning (2013)

Severe quetiapine poisoning produces hypotension primarily from alpha-adrenoreceptor antagonism. Methylene blue may have utility in the treatment of distributive shock resulting from poisoning refractory to standard vasopressor therapy.