gebhart/patrepeval huggingface dataset:
The role of<i>Six1</i>in mammalian auditory system development (2003)

The homeobox Six genes, homologues to Drosophila sine oculis(so) gene, are expressed in multiple organs during mammalian development. However, their roles during auditory system development have not been studied. We report that Six1 is required for mouse auditory system development. During inner ear development, Six1 expression was first detected in the ventral region of the otic pit and later is restricted to the middle and ventral otic vesicle within which, respectively, the vestibular and auditory epithelia form. By contrast, Six1 expression is excluded from the dorsal otic vesicle within which the semicircular canals form. Six1 is also expressed in the vestibuloacoustic ganglion. At E15.5, Six1 is expressed in all sensory epithelia of the inner ear. Using recently generated Six1 mutant mice, we found that all Six1+/- mice showed some degree of hearing loss because of a failure of sound transmission in the middle ear. By contrast, Six1-/- mice displayed malformations of the auditory system involving the outer, middle and inner ears. The inner ear development in Six1-/- embryos arrested at the otic vesicle stage and all components of the inner ear failed to form due to increased cell death and reduced cell proliferation in the otic epithelium. Because we previously reported that Six1 expression in the otic vesicle is Eya1dependent, we first clarified that Eya1 expression was unaffected in Six1-/- otic vesicle, further demonstrating that the Drosophila Eya-Six regulatory cassette is evolutionarily conserved during mammalian inner ear development. We also analyzed several other otic markers and found that the expression of Pax2 and Pax8 was unaffected in Six1-/- otic vesicle. By contrast, Six1 is required for the activation of Fgf3 expression and the maintenance of Fgf10 and Bmp4 expression in the otic vesicle. Furthermore, loss of Six1 function alters the expression pattern of Nkx5.1 and Gata3, indicating that Six1is required for regional specification of the otic vesicle. Finally, our data suggest that the interaction between Eya1 and Six1 is crucial for the morphogenesis of the cochlea and the posterior ampulla during inner ear development. These analyses establish a role for Six1 in early growth and patterning of the otic vesicle.
Macrocycles. 18. The Role of Cyclization in Syntheses of Poly(ether−sulfone)s (2001)

Poly(ether−sulfone)s having an identical backbone were prepared by four different methods. First, silylated bisphenol A (BSBA) was polycondensed with 4,4‘-difluorodiphenyl sulfone (DFDPS) and K2CO3 in N-methylpyrrolidone with variation of the temperature. Second, analogous polycondensation were conducted using CsF as catalyst (and no K2CO3). Third, CsF-catalyzed polycondensations BSBA and DFDPS were conducted in bulk up to 290 °C. Fourth, free bisphenol was polycondensed with DFDPS or 4,4‘-dichlorodiphenyl sulfone and K2CO3 in DMSO with azeotropic removal of water. MALDI−TOF mass spectroscopy revealed that the first method mainly yielded cyclic poly(ether−sulfone)s which were detected up to masses around 13 000 Da. These and other results suggest that these polycondensations take a kinetically kontrolled course at tempeatures ≤145 °C. This interpretation is also valid for the fourth method where high yields of cycles were obtained with DFDPS. With the less reactive 4,4‘-dichlorodiphenyl sulfone lower conversions, lower molecular weights and lower fractions of cycles were found. In contrast to KF (resulting from K2CO3) CsF cleaves the poly(ether sulfone) backbone at temperatures > 145 °C. Smaller amounts of smaller cycles were found in these CsF-catalyzed polycondensations which were in this case the result of thermodynamically controlled “back-biting degradation”.
Field effect potentiometric sensors (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTField effect potentiometric sensorsRichard P. Buck, David E. Hackleman, and William R. KenanCite this: Anal. Chem. 1977, 49, 14, 2315–2321Publication Date (Print):December 1, 1977Publication History Published online1 May 2002Published inissue 1 December 1977https://pubs.acs.org/doi/10.1021/ac50022a051https://doi.org/10.1021/ac50022a051research-articleACS PublicationsRequest reuse permissionsArticle Views251Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Hierarchical mixed-level simulation of VHDL descriptions (2002)

We present a hierarchical multilevel VHDL simulator for large systems described at the transistor, gate and higher levels. We exploit the hierarchy and regularity in VHDL descriptions to reduce the memory requirements drastically. The simulation algorithm handles MOS digital designs with bidirectional signal flow. We have augmented VHDL descriptions with signal strengths and timing; and also proposed a method to extend VHDL to accept transistor-level descriptions. Simulation results are provided for sample VHDL circuits.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Persecuting Alex Rodriguez (2014)

This qualitative textual analysis considers the US press coverage of Alex Rodriguez for his alleged use of performance-enhancing drugs. It evaluates nearly 500 newspaper, magazine and broadcast reports from 2007 to 2014 on Rodriguez, as well as reader and journalistic responses, and finds issues of overt and inferential racism, stereotyping and symbolic impurity, and a crude emphasis on money in the coverage. This research considers the ethics of the press coverage through a framework of Critical Race Theory and suggests an approach rooted in communitarian ethics to foster greater social justice and balance in sports media coverage.
PHYSIOLOGICAL ONTOGENY (1929)

We have studied by means of glass electrodes the hydrogen ion concentration of the blood of chicken embryos from 8 to 20 days. When plotted as a curve, the average data show that a constant change takes place in the measurement, being acid at the beginning of this period and becoming alkaline toward its end. The acid reaction we think was characteristic not only of the embryos of fowl, but of the fetuses of cats and indeed as Warburg has shown of rapidly growing tissue in general. We have suggested the possibility, though we have no data to substantiate the suggestion, that the hydrogen ion concentration may under conditions like these, seeing that the curve of change resembles that of oxygen consumption, be expressive of changes in the rate of metabolism.
Effects of Isosorbide Incorporation into Flexible Polyurethane Foams: Reversible Urethane Linkages and Antioxidant Activity (2019)

Isosorbide (ISB), a nontoxic bio-based bicyclic diol composed from two fuzed furans, was incorporated into the preparation of flexible polyurethane foams (FPUFs) for use as a cell opener and to impart antioxidant properties to the resulting foam. A novel method for cell opening was designed based on the anticipated reversibility of the urethane linkages formed by ISB with isocyanate. FPUFs containing various amounts of ISB (up to 5 wt%) were successfully prepared without any noticeable deterioration in the appearance and physical properties of the resulting foams. The air permeability of these resulting FPUFs was increased and this could be further improved by thermal treatment at 160 °C. The urethane units based on ISB enabled cell window opening, as anticipated, through the reversible urethane linkage. The ISB-containing FPUFs also demonstrated better antioxidant activity by impeding discoloration. Thus, ISB, a nontoxic, bio-based diol, can be a valuable raw material (or additive) for eco-friendly FPUFs without seriously compromising the physical properties of these FPUFs.
An approximate dynamic programming approach for coordinated charging control at vehicle-to-grid aggregator (2011)

A vehicle-to-grid (V2G) aggregator is an agent between the power grid and plug-in hybrid electrical vehicles (PHEVs). This paper studies the coordinated charging control of a V2G aggregator, which aims at minimizing the charging cost and reducing the power losses incurred by the fluctuating load. On one hand, a lower cost of charging gives the owners of PHEVs an incentive to cooperate. On the other hand, with an increasing popularity of PHEVs, the impact on the power grid such as power losses should be of concern to the aggregator. As an inherent property of a V2G aggregator, we enable bidirectional electric power flows between PHEVs and the power grid. Given the planned schedules of all the vehicles that are managed by an aggregator, we formulate the coordinated charging control as a dynamic programming problem. Due to the curse of dimensionality, we apply an approximate dynamic programming approach, which reduces the dimensionality of both state space and control space, to obtain the control sequences. We conduct simulations given the 24-hour schedules of 100 vehicles. Simulation results show that coordinated charging control can reduce both the total cost of charging and power losses significantly, compared with the scheme where each vehicle starts charging as soon as it is connected to the grid.
User Activity Analysis (2006)

This chapter contains sections titled: Step 1: Ask the Right Questions, Step 2: Look at What's Easily Available, Step 3: Figure Out What Extra Information You Need to Record, Exercise 1: See How the Other Half Lives, Security Risks of Running Programs in Response to a Web Request, Exercise 2: Comedy of Errors, Real-Time Error Notifications, Exercise 3: Talk to Your Client, Exercise 4: Design a Data Warehouse, More, Time and Motion
Modulation of humoral immune response through probiotic intake (2000)

Thirty healthy volunteers were randomised into three different treatment groups and consumed Lactobacillus GG, Lactococcus lactis or placebo (ethyl cellulose) for 7 days. On days 1, 3 and 5, an attenuated Salmonella typhi Ty21a oral vaccine was given to all subjects to mimic an enteropathogenic infection. All subjects responded well to the vaccine, but no significant differences were observed in numbers of IgA-, IgG- and IgM-secreting cells among the different groups. There was a trend towards a greater increase in specific IgA among the subjects receiving the vaccine in combination with Lactobacillus GG. Those receiving L. lactis with their vaccine evinced significantly higher CR3 receptor expression on neutrophils than those receiving either the placebo or Lactobacillus GG. These results indicate that probiotics may influence differently the immune response to oral S. typhi vaccine and that the immunomodulatory effect of probiotics is strain-dependent.
Basic and Clinical Aspects of Copper (2003)

An oxygen-rich atmosphere obligated living organisms to cope with reactive oxygen species (O2-, H2O2, OH*) that were the unavoidable by-products of cellular metabolism. As a redox cofactor Cu was selected as a co-catalyst for numerous biological processes, many involving the utilization of oxygen. Inadequate or excessive intake of Cu can be pathogenic and life-threatening. Mutations to genes that code for Cu-transporting ATPase enzymes are the molecular basis of Wilson and Menkes diseases and more recently Cu has been identified as a preemptory factor in amyloid and prion diseases. This review is dedicated to bringing historical and timely information on Cu transport, metabolism and homeostasis to the attention of those not familiar with this important mineral. Other comprehensive reviews are available to the interested readers.
A C-terminal motif found in the β <sub>2</sub> -adrenergic receptor, P2Y1 receptor and cystic fibrosis transmembrane conductance regulator determines binding to the Na <sup>+</sup> /H <sup>+</sup> exchanger regulatory factor family of PDZ proteins (1998)

The Na+/H+ exchanger regulatory factor (NHERF) binds to the tail of the beta2-adrenergic receptor and plays a role in adrenergic regulation of Na+/H+ exchange. NHERF contains two PDZ domains, the first of which is required for its interaction with the beta2 receptor. Mutagenesis studies of the beta2 receptor tail revealed that the optimal C-terminal motif for binding to the first PDZ domain of NHERF is D-S/T-x-L, a motif distinct from those recognized by other PDZ domains. The first PDZ domain of NHERF-2, a protein that is 52% identical to NHERF and also known as E3KARP, SIP-1, and TKA-1, exhibits binding preferences very similar to those of the first PDZ domain of NHERF. The delineation of the preferred binding motif for the first PDZ domain of the NHERF family of proteins allows for predictions for other proteins that may interact with NHERF or NHERF-2. For example, as would be predicted from the beta2 receptor tail mutagenesis studies, NHERF binds to the tail of the purinergic P2Y1 receptor, a seven-transmembrane receptor with an intracellular C-terminal tail ending in D-T-S-L. NHERF also binds to the tail of the cystic fibrosis transmembrane conductance regulator, which ends in D-T-R-L. Because the preferred binding motif of the first PDZ domain of the NHERF family of proteins is found at the C termini of a variety of intracellular proteins, NHERF and NHERF-2 may be multifunctional adaptor proteins involved in many previously unsuspected aspects of intracellular signaling.
A wide dynamic range compensated OTA for balanced filters (2002)

In this paper we propose an operational transconductance amplifier (OTA) with DC gain greater than 80 dB, improved frequency response and wide linear dynamic input range. Also, we propose a novel common mode feedback circuit, necessary for balanced filter common mode voltage stabilization. An elliptic low-pass filter simulated using this OTA exhibits a THD less than 1% while varying its cutoff frequency from 8 MHz to 12 MHz for a 0.8 V/sub pp/ differential input and /spl plusmn/2.5 V power supply.
Transcription factor HNF and hepatocyte differentiation (2008)

To know the precise mechanisms underlying the life or death and the regeneration or differentiation of cells would be relevant and useful for the development of a regenerative therapy for organ failure. Liver-specific gene expression is controlled primarily at a transcriptional level. Studies on the transcriptional regulatory elements of genes expressed in hepatocytes have identified several liver-enriched transcriptional factors, including hepatocyte nuclear factor (HNF)-1, HNF-3, HNF-4, HNF-6 and CCAAT/enhancer binding protein families, which are key components of the differentiation process for the fully functional liver. The transcriptional regulation by these HNFs, which form a hierarchical and cooperative network, is both essential for hepatocyte differentiation during mammalian liver development and also crucial for metabolic regulation and liver function. Among these liver-enriched transcription factors, HNF-4 is likely to act the furthest upstream as a master gene in transcriptional cascade and interacts with other liver-enriched transcriptional factors to stimulate hepatocyte-specific gene transcription. A link between the extracellular matrix, changes in cytoskeletal filament assembly and hepatocyte differentiation via HNF-4 has been shown to be involved in the transcriptional regulation of liver-specific gene expression. This review provides an overview of the roles of liver-enriched transcription factors in liver function.
Calphostins (UCN-1028), novel and specific inhibitors of protein kinase C. I. Fermentation, isolation, physico-chemical properties and biological activities. (1989)

A novel complex of calphostin (UCN-1028), which specifically inhibits protein kinase C (PKC) has been isolated from the culture broth of a fungi Cladosporium cladosporioides. Purification of individual components was carried out by silica gel, non-porus resin Diaion HP-20SS and Sephadex LH-20 chromatography, leading to isolation of five closely related components. A, B, C, D and I. Calphostins showed cytotoxic activities against various tumor cells, and these cytotoxicities were proportional to their inhibitory activities against PKC.
Functions of isolated domains of methionyl-tRNA synthetase from an extreme thermophile, Thermus thermophilus HB8. (1987)

Methionyl-tRNA synthetase (MetRS, 2 X 75 kDa) was purified to homogeneity from an extreme thermophile, Thermus thermophilus HB8. The polypeptide chain of MetRS was cleaved by limited digestion with trypsin into four domains: T1 (29 kDa), T2 (23 kDa), T3 (14.5 kDa), and T4 (7.5 kDa), which were aligned in that order. MetRS was also cleaved into similar fragments with a variety of other proteases. Domains T1, T2, T3, and T4 were isolated by column chromatography. "Tandem domain" T1-T2 (56 kDa) is fully active in the aminoacylation of tRNA and is further cleaved with trypsin into domains T1 and T2. Domain T1 is the smallest aminoacylation unit so far reported. Domain T2 (enzymatically inactive) interacts with tRNAMetf, as found by UV-induced cross-linking. Isolated domain T3 forms a dimer and is responsible for the dimer assembly of two protomers in MetRS. Domain T4 is a flexible tail of MetRS. These domains, in particular T1 and T2, will be important for detailed structure analyses in relation to aminoacylation activity.
V for Virtual (2006)

Operating system virtualization has been available on commodity hardware for a few years, and today attracts considerable commercial and research interest. Virtualization allows one or more virtual machines (VMs) to run on a single physical machine, and to interact via virtual devices, such as virtual hard discs or virtual network cards. To model basic virtualization operations, we propose a process calculus, V, with primitives to start and stop VMs, and to read and write data in a hierarchical store. Formalisms such as V may be useful for programming and reasoning about various applications of virtualization, such as VM-based trusted computing or VM-based computational grids.
A new model for the flat conductor electromagnetic SAW transducer (1978)

Previous models for Rayleigh- and Lamb-wave electromagnetic transducers have been based on the assumption that the current distribution in each transducer conductor is uniform. We present solutions for the dynamic magnetic fields surrounding an array of flat conductors above a ground plane in which the current distribution is allowed to vary. The resulting solutions are used to calculate space-harmonic curves and to derive a new transducer model including electrical inductance, eddy current resistance, acoustic impedance, and transducer efficiency. A direct analogy to the interdigital transducer is established. For fundamental frequency operation, this model is nearly equivalent to previous flat field models, provided the conductor width/spacing ratio is 1/2 or less. For harmonic transducer operation, the present analysis is substantially different from other models and it matches data more accurately. A method for measuring the individual insertion loss of dissimilar transducers in a lossy medium is presented.
CD73 as a novel marker for poor prognosis of oral squamous cell carcinoma (2016)

Ecto‑5'-nucleotidase [cluster of differentiation (CD)73] has important functions in several types of cancer, however, its expression in squamous cell carcinoma (SCC) remains unknown. The present study was designed to investigate CD73 expression in SCC. CD73 expression was assessed by immunohistochemistry in 113 patients with oral SCC (OSCC). The association between CD73 expression and clinicopathological features, overall survival (OS) and disease‑free survival (DFS) times of patients were statistically analyzed. CD73 expression was detected in 58.4% (66/113) of OSCC patients, with the immunostaining predominantly localized in the cytomembrane and a little in the cytoplasm. Statistical analysis revealed that CD73 expression was more frequently detected in patients with larger tumors (P=0.021). The overexpression of CD73 was significantly associated with clinical stage (P=0.047). Furthermore, immunohistochemical staining showed that overexpression of CD73 was inversely correlated with DFS (P=0.002) and OS (P=0.002) times. Multivariate Cox regression analysis revealed that CD73 expression was an independent prognostic factor for poor DFS (P=0.018) and OS (P=0.021). The current study is the first to evaluate the clinical significance and prognostic value of CD73 in patients with OSCC. The findings suggest that CD73 is a potential prognostic marker for OSCC.
TRPM3 channel expression and block in vascular smooth muscle cells (2008)

TRPM3 is a calcium‐permeable channel activated by sphingolipids, including D‐ erythro ‐sphingosine (Grimm et al 2005, Mol Pharmacol 67, 798–805). Its cellular functions are largely unknown. Using methods to develop isoform‐specific channel blockers (Xu et al 2005, Nat Biotechnol 23, 1289–93) we produced a TRPM3‐specific blocking agent (TM3E3). Preincubation of cells with TM3E3 produced about 40% block of TRPM3‐mediated calcium‐entry or ionic current. TM3E3 had no effect on the closely related channel TRPM2, or other TRP family members TRPC5 and TRPV4, confirming its specificity for TRPM3. Recently, novel activators for TRPM3 have been suggested. In conjunction with TM3E3, these activators have been used to study a potential role of TRPM3 in human vascular smooth muscle cells. The cells express mRNA encoding TRPM3 and the presence of TRPM3 is indicated by labelling with anti‐TRPM3 antibodies. Sphingosine and its analogues evoked calcium responses that could be inhibited by gadolinium, 2‐APB, TM3E3 or siRNA targeting TRPM3. The data suggest TRPM3 is a novel calcium‐entry channel of vascular smooth muscle cells. The research was supported by a BBSRC Industrial Co‐operative Award in Science and Engineering, the Wellcome Trust and the British Heart Foundation.