gebhart/patrepeval huggingface dataset:
Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis. (1994)

C1q, a subunit of the first component of the classical C pathway, binds to specific cells of the immune system, triggering a variety of cellular responses. To identify the functional C1qR on phagocytic cells, mAbs were generated by immunization with either C1q-binding proteins isolated from U937 cells or intact U937 cells. Immunoprecipitation followed by Western blot analysis demonstrated that three mAbs, designated R139 (IgG2b), R3 (IgM), and U40.3 (IgG1), recognize the same 100,000 M(r) protein (126,000 M(r) under reducing conditions). These mAbs also co-immunoprecipitate CD43 from detergent extracts of U937, consistent with the possibility that this C1qR is a multi-subunit structure. Two Abs, R3 and R139, but not U40.3, consistently inhibited the enhancement of phagocytosis by monocytes adhered to either C1q or the collagen-like fragment of C1q (C1q-CLF). Interestingly, binding inhibition studies demonstrated that neither R139 nor U40.3 blocked the binding of [125I]C1q-CLF to U937 cells, whereas R3 did inhibit 35 to 45% of the binding of [125I]C1q-CLF to these cells. Thus, the three mAbs recognize distinct epitopes of a 100,000 M(r) polypeptide that is a component of the monocyte C1qR that modulates phagocytosis. All three mAbs recognize the extracellular domain of the molecule on neutrophils, monocytes, and U937 cells, but were not reactive with CEM or RAJI cells, T and B lymphoblastoid cell lines, respectively. Furthermore, none of these three mAbs inhibited or mimicked the C1q-mediated stimulation of superoxide production by neutrophils, suggesting that the C1qR that mediates the enhancement of phagocytosis differs in at least some critical parameter from the C1qR that mediates superoxide generation by the neutrophil.
Alias-free interpolation technique for pulsed Doppler signal. (1993)

Conventional pulsed ultrasound Doppler technique has a fundamental limitation in the product of maximum velocity and maximum range because the Doppler shifted fre-quency is evaluated from the pulsed Doppler signal sampled at pulse repetition fre-quency, and the range is also restricted by pulse repetition frequency. This limitation sometimes causes the confusion in the measurement of blood flow velocity in a human heart. This paper describes a principle of alias-free interpolation technique for pulsed Doppler signal. This technique enables to synthesize the Doppler signal with high Nyquist frequency by interpolating the roughly spaced samples of the Doppler signal. The velocity range is expanded, and minimum sampling interval is not less than that in conventional technique, so that the range-velocity product can be expanded with this technique. Two cases of the interpolation are shown here. One is the interpolation for the single component Doppler signal and the other is for the two component Doppler signal. It is shown in simulation that these interpolation make the range-velocity product double.
Fluorescent Image Formation in the Fibre-optical Confocal Scanning Microscope (1992)

Abstract Reported in this paper are theoretical studies on two-dimensional and three-dimensional image formation with fluorescent objects in a fibre-optical confocal scanning microscope. An effective point spread function is introduced to derive the two- and three-dimensional optical transfer functions. It is found that, unlike confocal fluorescence microscopes with a finite circular detector, there is no missing cone of spatial frequencies, and no negative tail in the transfer function. The effect of the system parameters on the optical sectioning property is also investigated.
Maximum likelihood analysis of generalized linear models with missing covariates (1999)

Missing data is a common occurrence in most medical research data collection enterprises. There is an extensive literature concerning missing data, much of which has focused on missing outcomes. Covariates in regression models are often missing, particularly if information is being collected from multiple sources. The method of weights is an implementation of the EM algorithm for general maximum-likelihood analysis of regression models, including generalized linear models (GLMs) with incomplete covariates. In this paper, we will describe the method of weights in detail, illustrate its application with several examples, discuss its advantages and limitations, and review extensions and applications of the method.
Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 Regulates Tumor Necrosis Factor mRNA Stability and Translation Mainly by Altering Tristetraprolin Expression, Stability, and Binding to Adenine/Uridine-Rich Element (2006)

The mitogen-activated protein kinase (MAPK) p38/MAPK-activated protein kinase 2 (MK2) signaling pathway plays an important role in the posttranscriptional regulation of tumor necrosis factor (TNF), which is dependent on the adenine/uridine-rich element (ARE) in the 3' untranslated region of TNF mRNA. After lipopolysaccharide (LPS) stimulation, MK2-deficient macrophages show a 90% reduction in TNF production compared to the wild type. Tristetraprolin (TTP), a protein induced by LPS, binds ARE and destabilizes TNF mRNA. Accordingly, macrophages lacking TTP produce large amounts of TNF. Here, we generated MK2/TTP double knockout mice and show that, after LPS stimulation, bone marrow-derived macrophages produce TNF mRNA and protein levels comparable to those of TTP knockout cells, indicating that in the regulation of TNF biosynthesis TTP is genetically downstream of MK2. In addition, we show that MK2 is essential for the stabilization of TTP mRNA, and phosphorylation by MK2 leads to increased TTP protein stability but reduced ARE affinity. These data suggest that MK2 inhibits the mRNA destabilizing activity of TTP and, in parallel, codegradation of TTP together, with the target mRNA resulting in increased cellular levels of TTP.
Differences in activity of minoxidil and cyclosporin A on hair growth in nude and normal mice. Comparisons of in vivo and in vitro studies. (1990)

Hair growth effects of minoxidil and cyclosporin A were assessed in a series of experiments using nude mice. Systematic monitoring of coat hair showed that untreated nude mice grow extremely sparse and transient hair in cycles. This monitoring was done by photographing each animal through at least one full growth cycle and rating peak growth on a 1 to 4 scale. Topical administration of minoxidil or minoxidil sulfate did not influence this cyclic hair growth. Orally administered minoxidil also had no effect but oral cyclosporin A increased peak hair growth. None of the treatments altered the length of the hair cycle. Direct drug effects on follicles were tested in vitro using organ cultured vibrissae from both nude and normal mice. Minoxidil stimulated hair growth in follicles from normal but not nude mice. In contrast, cyclosporin A stimulated growth only in vibrissae follicles from nude but not normal animals. These studies show that minoxidil and cyclosporin A influence hair growth differentially. Cyclosporin A directly affects nude hair follicles by apparently compensating for a genetic defect inherent in nude follicles. Minoxidil does not have a similar effect. Apparently, the biochemical pathway activated by minoxidil is not a critical defect of hair growth in nude mice. We conclude that nude mice are not useful for studying minoxidil effects but they may be useful in studying pleiotropic effects of the nude gene on hair growth.
Catalytic Properties of Fluorine Modified SmNa-Y Zeolites (1992)

It is known that mild fluorination of zeolites, including those modified with polyvalent cations, increases their activity for acid catalyzed reactions. In this study we report the reactivity of fluorine modified Sm(III) exchanged zeolite NaY. Mild fluorination of SmNaY reduces the activity of strong protonic acid sites and increases the contribution of weak aprotic acid centres. Results for cumene cracking and toluene disproportionation as well as for 1-hexene isomerization are reported.
Alumina-based Coating for Coke Reduction in Steam Crackers (2020)

Alumina-based coatings have been claimed as being an advantageous modification in industrial ethylene furnaces. In this work, on-line experimentally measured coking rates of a commercial coating (CoatAlloy™) have pointed out its superiority compared to an uncoated reference material in an electrobalance set-up. Additionally, the effects of presulfiding with 500 ppmw DMDS per H<sub>2</sub>O, continuous addition of 41 ppmw S per HC of DMDS, and a combination thereof were evaluated during ethane steam cracking under industrially relevant conditions (T<sub>gasphase</sub> = 1173 K, P<sub>tot</sub> = 0.1 MPa, X<sub>C2H6</sub> = 70%, dilution δ = 0.33 kg<sub>H2O</sub>/kg<sub>HC</sub>). The examined samples were further evaluated using online thermogravimetry, scanning electron microscopy and energy diffractive X-ray for surface and cross-section analysis together with X-ray photoelectron spectroscopy and wavelength-dispersive X-ray spectroscopy for surface analysis. The passivating coating illustrated a better performance than the reference Ni-Cr Fe-base alloy after application of an improved pretreatment, followed by piddling changes on the product distribution. Presulfiding of the coating affected negatively the observed coking rates in comparison with the reference alloy, so alternative presulfiding and sulfur addition strategies are recommended when using this barrier coating.
Synthesis and solution imidization studies of soluble poly(imide siloxane) segmented copolymers (1987)

Soluble metalinked poly(imide siloxane) segmented copolymers were synthesized utilizing a THF/NMP cosolvent system. The presence of dual solvent makes it possibled to reach high molecular weight in the amid acid stage. Incorporation of siloxanes at about 10 weight percent or higher enables the materials to be fully soluble in a range of polar solvents even after imidization. Imidization may be achieved either by conventional thermal methods on cast amic acid films or in appropriate solvent/azeotroping-agent systems employing moderate temperatures. The imidization procedure has been followed by FT-IR and NMR studies. FT-IR studies show the solution imidization follows first order kinetics and proceeds to about 96 percent completion. NMR studies of the isolated products show residual amic acid may be present after solution imidization, but only at very low levels. Properties of the solution imidized materials compared well with those obtained from samples imidized as thin films.
Synthetic Utility of Copper(I) Phenoxfde Complexes (1989)

Several sulphur containing organic molecules react with copper(I) phenoxides under extremely mild conditions to give cuprous sulphide and an organic phenoxide. The reaction proceeds through nucleophilic substitution on the coordinated sulphur compound. The nature of the product obtained is governed by the ancillary ligands around copper(I). The high yields of the organic product obtained make these reactions extremely attractive as methods of synthesis for these compounds.
Single molecule actuation and detection on a lab-on-a-chip magnetoresistive platform (2011)

On-chip magnetic tweezers based on current loops were integrated with magnetoresistive sensors. Magnetic forces up to 1.0±0.3pN are produced to actuate on DNA anchored to the surface of a flow cell and labeled with micrometer-sized magnetic beads. The levitation of the beads stretches the immobilized DNA. The relative position of the magnetic beads is monitored using spin-valve sensors. A bead vertical displacement resolution of 60nm is derived for DNA molecular motor activity in a tweezer steady current regime.
PGP in constrained wireless devices (2000)

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On the life history of Cape horse mackerel<i>Trachurus trachurus capensis</i>off the south-east coast of South Africa (1990)

Cape horse mackerel on the south-east coast of South Africa attain a maximum age of 10 years. One opaque and one hyaline zone are deposited on the otoliths per year and growth is described by the equation Lt = 503 (1 − e−0,427(t + 0,194)) mm. Fifty-per-cent sexual maturity is attained in the second year, between 320 and 330 mm total length (TL). Horse mackerel first recruit to the fishery in their second year, although 50-per-cent recruitment only occurs at 440 mm TL, at five years of age. Throughout each year the size distribution of the catch is unimodal, between 440 and 499 mm TL, the age of the fish ranging from five to nine years. The reproductive season extends from June to November during which there are two spawning peaks, and immediately after spawning the gonads undergo a heavy nematode infestation. Sexes are evenly distributed at a ratio of 1 : 1. Along the South-East Coast the species filter-feeds non-specifically on zooplankton. The findings of this study support the hypotheses that adult Cape horse mackerel undertake a spawning migration and that the fish on South Africa's south-east and west coasts and those off southern Namibia are a single stock.
Cardiac Expression of a Mini-dystrophin That Normalizes Skeletal Muscle Force Only Partially Restores Heart Function in Aged Mdx Mice (2008)

Duchenne muscular dystrophy (DMD) affects both skeletal and cardiac muscle. It is currently unclear whether the strategies developed for skeletal muscle can ameliorate cardiomyopathy. Synthetic mini-/micro-dystrophin genes have yielded impressive skeletal muscle protection in animal models. The 6-kb DeltaH2-R19 minigene is particularly promising because it completely restores skeletal muscle force to wild-type levels. Here, we examined whether expressing this minigene in the heart, but not skeletal muscle, could normalize cardiac function in the mdx model of DMD cardiomyopathy. Transgenic mdx mice were generated to express the DeltaH2-R19 minigene under the control of the alpha-myosin heavy-chain promoter. Heart structure and function were examined in adult and very old mice. The DeltaH2-R19 minigene enhanced cardiomyocyte sarcolemmal strength and prevented myocardial fibrosis. It also restored the dobutamine response and enhanced treadmill performance. Surprisingly, heart-restricted DeltaH2-R19 minigene expression did not completely normalize electrocardiogram and hemodynamic abnormalities. Overall, systolic function and ejection fraction were restored to normal levels but stroke volume and cardiac output remained suboptimal. Our results demonstrate that the skeletal muscle-proven DeltaH2-R19 minigene can correct cardiac histopathology but cannot fully normalize heart function. Novel strategies must be developed to completely restore heart function in DMD.
Entity Identification in Database Integration: An Evidential Reasoning Approach (1993)

Entity identification is the problem of matching object instances from different databases which correspond to the same real-world entity. In this paper, we present a 2-step entity identification process in which attributes for matching tuples may be missing in certain tuples, and thus need to be derived prior to the matching. To match tuples, we require identity rules which specify the conditions to be satisfied by a pair of tuples, from different databases, before they can be considered as modeling the same real-world entity. We also introduce ILFD&apos;s (instance-level functional dependencies) as a form of inference rules which derive the missing identifying attributes. In order to provide more interesting integrated results to the users, we allow both identity rules and ILFD&apos;s to contain indefiniteness represented as necessary and possible support information. Based on support logic programming[2], we develop an approach to perform reasoning on the local databases using identity rules ...
Impact of GLP-1 Receptor Agonists on Major Gastrointestinal Disorders for Type 2 Diabetes Mellitus: A Mixed Treatment Comparison Meta-Analysis (2012)

Our MTC meta-analysis suggests that patients should be warned about these GIAEs in early stage of treatment by GLP-1s, especially by EX10BID and LIR1.2, to promote treatment compliance.
Regulation of sphingosine 1‐phosphate‐induced endothelial cytoskeletal rearrangement and barrier enhancement by S1P <sub>1</sub> receptor, PI3 kinase, Tiam1/Rac1, and α‐actinin (2005)

Endothelial cell (EC) barrier dysfunction results in increased vascular permeability observed in inflammation, tumor angiogenesis, and atherosclerosis. The platelet-derived phospholipid sphingosine-1-phosphate (S1P) decreases EC permeability in vitro and in vivo and thus has obvious therapeutic potential. We examined S1P-mediated human pulmonary artery EC signaling and barrier regulation in caveolin-enriched microdomains (CEM). Immunoblotting from S1P-treated EC revealed S1P-mediated rapid recruitment (1 microM, 5 min) to CEMs of the S1P receptors S1P1 and S1P3, p110 PI3 kinase alpha and beta catalytic subunits, the Rac1 GEF, Tiam1, and alpha-actinin isoforms 1 and 4. Immunoprecipitated p110 PI3 kinase catalytic subunits from S1P-treated EC exhibited PIP3 production in CEMs. Immunoprecipitation of S1P receptors from CEM fractions revealed complexes containing Tiam1 and S1P1. PI3 kinase inhibition (LY294002) attenuated S1P-induced Tiam1 association with S1P1, Tiam1/Rac1 activation, alpha-actinin-1/4 recruitment, and EC barrier enhancement. Silencing of either S1P1 or Tiam1 expression resulted in the loss of S1P-mediated Rac1 activation and alpha-actinin-1/4 recruitment to CEM. Finally, silencing S1P1, Tiam1, or both alpha-actinin isoforms 1/4 inhibits S1P-induced cortical F-actin rearrangement and S1P-mediated barrier enhancement. Taken together, these results suggest that S1P-induced recruitment of S1P1 to CEM fractions promotes PI3 kinase-mediated Tiam1/Rac1 activation required for alpha-actinin-1/4-regulated cortical actin rearrangement and EC barrier enhancement.
Polymer–Liposome Complexes with a Functional Hydrogen-Bond Cross-Linker for Preventing Protein Adsorption and Improving Tumor Accumulation (2013)

To solve biostability and organ distribution problems in polymer-coated liposomes, this study developed tumor–extracellular matrix pH-induced targeting polymer–liposome complexes (ECM-targeting liposomes) that are highly stable in a protein-rich environment and can trigger targeting ability in tumor ECM environment. Experimental results show that the ECM-targeting liposomes significantly reduced adsorption of various proteins (HSA, IgG, and fibrinogen) and leakage of encapsulated drug doxorubicin–hydrochloride from liposomes, significantly improved uptake efficiency in HCT116 colon cancer cells, improved HCT116 tumor accumulation, and reduced distribution in normal organs (e.g., liver, spleen, lung, etc.). We demonstrate that ECM-targeting liposomes overcome the limitations of conventional liposomes and stealth liposomes in cancer therapy.
Negative Incidence Flow Over a Turbine Rotor Blade (1983)

The operation of variable cycle gas turbines at negative incidence can result in highly three dimensional separated flows on the turbine rotor pressure surface. These flows can impact both performance and durability. The present program was conducted to experimentally study the behavior of surface flow on a large scale axial flow turbine rotor with incidence varying up to and including negative incidence separation. Fullspan pressure distributions and surface flow visualization were acquired over a range of incidence. The data indicate that at large negative incidence, pressure surface separation occurred and extended to 60 percent chord at midspan. These separated flows were simulated at midspan by applying potential flow theory to match the measured pressure distributions.
Cells and Organs on Chip—A Revolutionary Platform for Biomedicine (2016)

Lab‐on‐a‐chip (LOC) and microfluidics are important technologies with numerous applications from drug delivery to tissue engineering. LOC integrates fluidic and electronic components on a single chip and becomes very attractive due to the possibility of their state‐of‐art implementation in personalized devices for the point‐of‐care treatments. Microfluidics is the technique that deals with small (10-9 to 10-18 L) amounts of fluids, using channels with dimensions of 10 to 100 μm. These LOC and microfluidics devices enable the development of next‐generation portable and implantable bioelectronics devices. Superior chip‐based technologies are emerging with the advances in microfluidics and motivating various chip‐based methods for rapid low‐cost analysis as compared to traditional laboratory method.An organ‐on‐chip (OOC) is on‐chip cell culture device created with microfabrication techniques and contains continuously perfused chambers inhabited by living cells that simulate tissue‐ and organ‐level physiology. In vitro models of cells, tissues and organ based on LOC devices are a major breakthrough for research in biologic systems and mechanisms. The recapitulations of cellular events in OOC devices provide them an edge over two‐dimensional (2D) and three‐dimensional (3D) cultures and open a gateway for their newer applications in biomedicine such as tissue engineering, drug discovery and disease modeling. In this chapter, the advancement and potential applications of OOC devices are discussed.
Multitaper Time-Frequency Reassignment for Nonstationary Spectrum Estimation and Chirp Enhancement (2007)

A method is proposed for obtaining time-frequency distributions of chirp signals embedded in nonstationary noise, with the two-fold objective of a sharp localization for the chirp components and a reduced level of statistical fluctuations for the noise. The technique consists in combining time-frequency reassignment with multitapering, and two variations are proposed. The first one, primarily aimed at nonstationary spectrum estimation, is based on sums of estimates with different tapers, whereas the second one makes use of differences between the same estimates for the sake of chirp enhancement. The principle of the technique is outlined, its implementation based on Hermite functions is justified and discussed, and some examples are provided for supporting the efficiency of the approach, both qualitatively and quantitatively
Comparison of human tenascin expression in normal, Simian‐virus‐40‐transformed and tumor‐derived cell lines (1992)

Tenascin is a polymorphic high-molecular-mass extracellular-matrix glycoprotein composed of six similar subunits. Using two-domain-specific anti-tenascin monoclonal antibodies, we have studied the expression and distribution of tenascin in four cultured normal human fibroblasts, two simian-virus-40-(SV40)-transformed and three tumor-derived (melanoma, rhabdomyosarcoma and fibrosarcoma) cell lines. We found that (a) cultured normal human fibroblasts accumulate considerable amounts of tenascin and retain 60-90% in the extracellular matrix, while they release the remainder into the tissue-culture medium; (b) of the two SV40-transformed counterparts we have tested, the AG-280 cell line accumulates no detectable amounts of tenascin and the WI-38-VA cell line accumulates about 10-times less tenascin than its normal counterpart and releases about 90% of it into the culture medium; (c) some tumor-derived cell lines accumulate considerable amounts of tenascin, but in these cases, more than 90% is released into the culture media; (d) in normal human fibroblasts, two major tenascin isoforms, generated by alternative splicing of the mRNA precursor, are detectable (280 kDa and 190 kDa, respectively) and the lower-molecular-mass tenascin isoform is accumulated preferentially in the extracellular matrix; (e) in SV40-transformed or tumor-derived cell lines, only the higher-molecular-mass isoform is detectable and it is more sialylated than the tenascin produced by the normal human fibroblast cell lines.