gebhart/patrepeval huggingface dataset:
Genotype–Phenotype Correlations in Autosomal Dominant and Autosomal Recessive Polycystic Kidney Disease (2007)

The phenotypes that are associated with the common forms of polycystic kidney disease (PKD)--autosomal dominant (ADPKD) and autosomal recessive (ARPKD)--are highly variable in penetrance. This is in terms of severity of renal disease, which can range from neonatal death to adequate function into old age, characteristics of the liver disease, and other extrarenal manifestations in ADPKD. Influences of the germline mutation are at the genic and allelic levels, but intrafamilial variability indicates that genetic background and environmental factors are also key. In ADPKD, the gene involved, PKD1 or PKD2, is a major factor, with ESRD occurring 20 yr later in PKD2. Mutation position may also be significant, especially in terms of the likelihood of vascular events, with 5' mutations most detrimental. Variance component analysis in ADPKD populations indicates that genetic modifiers are important, but few such factors (beyond co-inheritance of a TSC2 mutation) have been identified. Hormonal influences, especially associated with more severe liver disease in female individuals, indicate a role for nongenetic factors. In ARPKD, the combination of mutations is critical to the phenotypic outcome. Patients with two truncating mutations have a lethal phenotype, whereas the presence of at least one missense change can be compatible with life, indicating that many missense changes are hypomorphic alleles that generate partially functional protein. Clues from animal models and other forms of PKD highlight potential modifiers. The information that is now available on both genes is of considerable prognostic value with the prospects from the ongoing genetic revolution that additional risk factors will be revealed.
Extensive variations and basic features in the alcohol dehydrogenase – sorbitol dehydrogenase family (1984)

Structural comparisons of sorbitol dehydrogenase with zinc-containing 'long' alcohol dehydrogenases reveal distant but clear relationships. An alignment suggests 93 positional identities with horse liver alcohol dehydrogenase (25% of 374 positions) and 73 identities with yeast alcohol dehydrogenase (20%). Sorbitol dehydrogenase forms a link between these distantly related alcohol dehydrogenases and is in some regions more similar to one of them that they are to each other. 43 residues (11%) are common to all three enzymes and include a heavy over-representation of glycine (half of all glycine residues in sorbitol dehydrogenase), showing the importance of space restrictions in protein structures. Four regions are well conserved, two in each domain of horse liver alcohol dehydrogenase. They are two segments close to the active-site zinc atom of the catalytic domain, and two in the central beta-pleated sheet strands of the coenzyme-binding domain. These similarities demonstrate the general importance of internal and central building units in proteins. Large variations affect a region adjacent to the third protein ligand to the active-site zinc atom in horse liver alcohol dehydrogenase. Such changes at active sites of related enzymes are unusual. Other large differences concern the segment around the non-catalytic zinc atom of horse liver alcohol dehydrogenase; three of its four cysteine ligands are absent from sorbitol dehydrogenase. Three segments with several exchanges correspond to a continuous region with superficial areas, inter-domain contacts and inter-subunit interactions in the catalytic domain of alcohol dehydrogenase. They may correlate with the altered quaternary structure of sorbitol dehydrogenase. Regions corresponding to top and bottom beta-strands in the coenzyme-binding domain of the alcohol dehydrogenase are also little conserved. Within sorbitol dehydrogenase, a large segment shows an internal similarity. The two distantly related alcohol dehydrogenases and sorbitol dehydrogenase form a triplet of enzymes illustrating basic protein relationships. They are ancestrally close enough to establish similarities, yet sufficiently divergent to illustrate changes in all but fundamental properties.
Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism. (1988)

S-Adenosyl-L-methionine:demethylmacrocin O-methyltransferase catalyzes the conversion of demethylmacrocin to macrocin as the penultimate step of tylosin biosynthesis in Streptomyces fradiae. The O-methyltransferase was purified to electrophoretic homogeneity by a conventional chromatographic procedure. The purified enzyme appears to be trimeric with a molecular weight of 122,000-126,000 and a subunit size of 42,000. Its isoelectric point was 6.0. The enzyme required Mg2+ for maximal activity and was catalytically optimal at pH 7.8-8.5 and 42 degrees C. The O-methyltransferase catalyzed conversion of demethylmacrocin to macrocin at a stoichiometric ratio of 1:1. The O-methyltransferase also mediated conversion of demethyllactenocin----lactenocin. The corresponding Vmax/Km ratios for the two analogous conversions varied only slightly. Both enzymic conversions were susceptible to an extensive and identical range of metabolic inhibitions. Steady-state kinetic studies for initial velocity, substrate analogue, and product inhibitions are consistent with Ordered Bi Bi as the reaction mechanism of demethylmacrocin O-methyltransferase. Except for an identical kinetic mechanism, demethylmacrocin O-methyltransferase can be readily differentiated from macrocin O-methyltransferase by its physical and catalytic properties as well as metabolic inhibitions.
Single bubble generated by a pulsed discharge in liquids as a plasma microreactor (2011)

We discuss a single bubble, generated by a pulsed discharge in liquids, in which reactive plasmas interact with gases and liquids. Double-pulsed microdischarges with variable time intervals were used. The first microdischarge was used to generate the bubble and the second one was used to create plasmas in it, which worked as a microcavity for plasma processing. The bubble remained for a period of 400–600 µs, which was affected by discharge energy. The electrode erosion caused by the discharge in or without the bubble was characterized.
Fast dynamic liquid level sensor based on liquid resistance (2007)

Based on the characteristics of liquid resistance, a new transient liquid sensor has been designed by means of ratio measurement. A new AC exciting constant current source is used as excitation source for the sensor, which not only maintains all features of an ideal exciting constant current source in terms of accuracy, disturbance rejection and stability, but also solves the problem arising from the polarization effect by changing the current directions alternatively. Analyzing the influence on the characteristics of liquid resistance under different temperature and medium, the model of the sensor is built and the model parameters are determined. We adopt the single-chip microprocessor as the measuring unit and control unit of the sensor. Both theoretical analysis and experimental results are presented to show the effectiveness of the sensor. Results show that it is sensitive enough to trace the variability of the liquid level.
Phenomenological Studies in Laser Cladding. Part I. Time-Resolved Measurements of the Absorptivity of Metal Powder (1993)

Time-resolved measurements were introduced to study the dynamic behavior of the absorptivity of metal powder under CO 2 laser irradiation conditions. The absorptivity of metal powder was more than 90% under short-term laser irradiation (6-20 ms), but it was below 30% under relatively long-term laser irradiation (50-1000 ms). The experimental results propose that the dynamic change of the absorptivity is caused by time-dependent aggregation of powder particles induced by laser irradiation.
Cobalt-dithiolene complexes for the photocatalytic and electrocatalytic reduction of protons in aqueous solutions (2012)

Artificial photosynthesis (AP) is a promising method of converting solar energy into fuel (H(2)). Harnessing solar energy to generate H(2) from H(+) is a crucial process in systems for artificial photosynthesis. Widespread application of a device for AP would rely on the use of platinum-free catalysts due to the scarcity of noble metals. Here we report a series of cobalt dithiolene complexes that are exceptionally active for the catalytic reduction of protons in aqueous solvent mixtures. All catalysts perform visible-light-driven reduction of protons from water when paired with Ru(bpy)(3)(2+) as the photosensitizer and ascorbic acid as the sacrificial donor. Photocatalysts with electron withdrawing groups exhibit the highest activity with turnovers up to 9,000 with respect to catalyst. The same complexes are also active electrocatalysts in 11 acetonitrile/water. The electrocatalytic mechanism is proposed to be ECEC, where the Co dithiolene catalysts undergo rapid protonation once they are reduced to CoL(2)(2-). Subsequent reduction and reaction with H(+) lead to H(2) formation. Cobalt dithiolene complexes thus represent a new group of active catalysts for the reduction of protons.
A phase-sensitive optical time-domain reflectometry system with an electrical I/Q demodulator (2018)

We propose an electrical I/Q demodulation scheme to decrease the sampling rate and computational cost in coherent phase-sensitive Optical Time-Domain Reflectometry systems. The IF signal from the photon detector and the local-oscillator from the acoustic-optic modulator driver are splited into two parts, respectively. One of local-oscillators is 90°shifted, then the signals and LOs are cross-mixed and the outputs are low-pass filtered to obtain the I/Q signal. Besides of the save of computational cost, the sampling rate and data storage is reduced at least 1.6 times. At last, a quantitative measurement of a 50 Hz vibration is successfully demonstrated in the experiment.
Neutral Deinking Technology. Benefit & Problems Solution of The Problems by Micro-Floatation, Etc. (1995)

Deinking Technology has been rapidly improved specially in the field of hardware, such as High Consistency Pulping, Fine Slot Screening, Dispersing, and Post-Flotation etc.. However Almost all of the deinking process are still operated under Alkali base, which is still common technology for the deinking.In order to improve sticky problems, Aikawa has recommended two loops deinking system which consists of Neutral, Room Temperature loop for the first half of the system and Alkali, High Temperature loop for the latter half of the system since several years ago. In Europe, very recently, the first Neutral deinging system was started in an actual paper mill, which shows some advantages to protect the pollution of environment and to save production cost in spite of some disadvantages. This paper shows these advantages and problems for the Neutral Deinking Technology.
ABC Transporters in <i>Saccharomyces cerevisiae</i> and Their Interactors: New Technology Advances the Biology of the ABCC (MRP) Subfamily (2009)

Members of the ATP-binding cassette (ABC) transporter superfamily exist in bacteria, fungi, plants, and animals and play key roles in the efflux of xenobiotic compounds, physiological substrates, and toxic intracellular metabolites. Based on sequence relatedness, mammalian ABC proteins have been divided into seven subfamilies, ABC subfamily A (ABCA) to ABCG. This review focuses on recent advances in our understanding of ABC transporters in the model organism Saccharomyces cerevisiae. We propose a revised unified nomenclature for the six yeast ABC subfamilies to reflect the current mammalian designations ABCA to ABCG. In addition, we specifically review the well-studied yeast ABCC subfamily (formerly designated the MRP/CFTR subfamily), which includes six members (Ycf1p, Bpt1p, Ybt1p/Bat1p, Nft1p, Vmr1p, and Yor1p). We focus on Ycf1p, the best-characterized yeast ABCC transporter. Ycf1p is located in the vacuolar membrane in yeast and functions in a manner analogous to that of the human multidrug resistance-related protein (MRP1, also called ABCC1), mediating the transport of glutathione-conjugated toxic compounds. We review what is known about Ycf1p substrates, trafficking, processing, posttranslational modifications, regulation, and interactors. Finally, we discuss a powerful new yeast two-hybrid technology called integrated membrane yeast two-hybrid (iMYTH) technology, which was designed to identify interactors of membrane proteins. iMYTH technology has successfully identified novel interactors of Ycf1p and promises to be an invaluable tool in future efforts to comprehensively define the yeast ABC interactome.
Practical aspects of calender steam showers. (1988)

Le traitement de la feuille par la vapeur lors du calandrage ameliore la reponse au calandrage, avec des ameliorations de 10 a 20% du lisse, de meilleurs profils transversaux de grammage et l'elimination des differences entre les deux faces de la feuille
Direct and reversible inhibition of estradiol-stimulated prolactin synthesis by antiestrogens in vitro. (1983)

The antiestrogens tamoxifen and monohydroxytamoxifen inhibited the estradiol-stimulated increase in prolactin synthesis by dispersed cells in culture derived from immature rat pituitary glands. Monohydroxytamoxifen had a relative binding affinity for the estrogen receptor similar to that of estradiol, whereas tamoxifen's relative binding affinity was approximately 3%. This was consistent with the observation that monohydroxytamoxifen was 30 times more potent than tamoxifen as an antiestrogen in vitro. To avoid the possibility that tamoxifen was fractionally metabolized to monohydroxytamoxifen by the pituitary cells, the p-methyl, p-chloro, and p-fluoro derivatives of tamoxifen that are unlikely to be converted to monohydroxytamoxifen were tested for activity. The substitution did not have a detrimental effect on their ability to inhibit the binding of [3H]estradiol to either rat uterine or pituitary gland estrogen receptors. Similarly, the derivatives of tamoxifen inhibited estradiol-stimulated prolactin synthesis at concentrations that were consistent with their relative binding affinities. Although it is clearly an advantage for tamoxifen to be metabolized to the more potent antiestrogen monohydroxytamoxifen, we have shown that this is not a prerequisite for the antiestrogenic actions of tamoxifen. With the direct actions of antiestrogens established, the pituitary cell system was validated for further structure-activity relationship studies. Overall, the inhibition of estradiol-stimulated prolactin synthesis by antiestrogens is competitive and reversible with estradiol, an effect that can be explained by interactions with the estrogen receptor system.
Technology licensing in China (2014)

We explore the landscape of technology licensing among Chinese entities in the period 2000–12, using a unique database on technological licensing from the State Intellectual Property Office of China. We find that: first, among Chinese licensee organizations, firms have dominated in terms of the number of licensed technologies; second, the geographical distribution of licensed technologies among the provinces has gradually reached a new quantitative balance; third, utility models are the most popular technologies to be licensed and the majority of technology licensing in China has been between Chinese entities, and most transactions have been local within provinces; and finally, Chinese firms have gradually in-licensed newer and newer technologies, but the technologies in-licensed from foreign sources are by no means state-of-the-art. We make several suggestions for innovation policy-making and for directions for futureresearch.
Simultaneous T1 and T2 Brain Relaxometry in Asymptomatic Volunteers Using Magnetic Resonance Fingerprinting (2015)

Magnetic resonance fingerprinting (MRF) is a method of image acquisition that produces multiple MR parametric maps from a single scan. Here, we describe the normal range and progression of MRF-derived relaxometry values with age in healthy individuals. 56 normal volunteers (ages 11-71 years, M:F 24:32) were scanned. Regions of interest were drawn on T<sub>1</sub> and T<sub>2</sub> maps in 38 areas, including lobar and deep white matter, deep gray nuclei, thalami and posterior fossa structures. Relaxometry differences were assessed using a forward stepwise selection of a baseline model including either gender, age, or both, where variables were included if they contributed significantly (p<0.05). Additionally, differences in regional anatomy, including comparisons between hemispheres and between anatomical subcomponents, were assessed by paired t-tests. Using this protocol, MRF-derived T<sub>1</sub> and T<sub>2</sub> in frontal WM regions were found to increase in with age, while occipital and temporal regions remained relatively stable. Deep gray nuclei, including substantia nigra, were found to have age-related decreases in relaxometry. Gender differences were observed in T<sub>1</sub> and T<sub>2</sub> of temporal regions, cerebellum and pons. Males were also found to have more rapid age-related changes in frontal and parietal WM. Regional differences were identified between hemispheres, between genu and splenium of corpus callosum, and between posteromedial and anterolateral thalami. In conclusion, MRF quantification can measure relaxometry trends in healthy individuals that are in agreement with current understanding of neuroanatomy and neurobiology, and has the ability to uncover additional patterns that have not yet been explored.
How to detect grammatical errors in a text without parsing it (1987)

The Constituent Likelihood Automatic Word-tagging System (CLAWS) was originally designed for the low-level grammatical analysis of the million-word LOB Corpus of English text samples. CLAWS does not attempt a full parse, but uses a first-order Markov model of language to assign word-class labels to words. CLAWS can be modified to detect grammatical errors, essentially by flagging unlikely word-class transitions in the input text. This may seem to be an intuitively implausible and theoretically inadequate model of natural language syntax, but nevertheless it can successfully pinpoint most grammatical errors in a text. Several modifications to CLAWS have been explored. The resulting system cannot detect all errors in typed documents; but then neither do far more complex systems, which attempt a full parse, requiring much greater computation.
Enantioselective syntheses with titanium carbohydrate complexes. Part 7. Enantioselective allyltitanation of aldehydes with cyclopentadienyldialkoxyallyltitanium complexes (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnantioselective syntheses with titanium carbohydrate complexes. Part 7. Enantioselective allyltitanation of aldehydes with cyclopentadienyldialkoxyallyltitanium complexesAndreas Hafner, Rudolf O. Duthaler, Roger Marti, Grety Rihs, Petra Rothe-Streit, and Franz SchwarzenbachCite this: J. Am. Chem. Soc. 1992, 114, 7, 2321–2336Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/ja00033a005https://doi.org/10.1021/ja00033a005research-articleACS PublicationsRequest reuse permissionsArticle Views3434Altmetric-Citations313LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Identification and characterization of phage‐resistance genes in temperate lactococcal bacteriophages (2002)

The sie2009 gene, which is situated between the genes encoding the repressor and integrase, on the lysogeny module of the temperate lactococcal bacteriophage Tuc2009, was shown to mediate a phage-resistance phenotype in Lactococcus lactis against a number of bacteriophages. The Sie2009 protein is associated with the cell membrane and its expression leaves phage adsorption, transfection and plasmid transformation unaffected, but interferes with plasmid transduction, as well as phage replication. These observations indicate that this resistance is as a result of DNA injection blocking, thus representing a novel superinfection exclusion system. A polymerase chain reaction (PCR)-based strategy was used to screen a number of lactococcal strains for the presence of other prophage-encoded phage-resistance systems. This screening resulted in the identification of two such systems, without homology to sie2009, which were shown to mediate a phage-resistance phenotype similar to that conferred by sie2009. To our knowledge, this is the first description of a phage-encoded super-infection exclusion/injection blocking mechanism in the genus Lactococcus.
TAR RNA loop: A scaffold for the assembly of a regulatory switch in HIV replication (2002)

Replication of HIV requires the Tat protein, which activates elongation of RNA polymerase II transcription at the HIV-1 promoter by interacting with the cyclin T1 (CycT1) subunit of the positive transcription elongation factor complex b (P-TEFb). The transactivation domain of Tat binds directly to the CycT1 subunit of P-TEFb and induces loop sequence-specific binding of P-TEFb onto nascent HIV-1 trans-activation responsive region (TAR) RNA. We used systematic RNA-protein photocross-linking, Western blot analysis, and protein footprinting to show that residues 252-260 of CycT1 interact with one side of the TAR RNA loop and enhance interaction of Tat residue K50 to the other side of the loop. Our results show that TAR RNA provides a scaffold for two protein partners to bind and assemble a regulatory switch in HIV replication. RNA-mediated assembly of RNA-protein complexes could be a general mechanism for stable ribonucleoprotein complex formation and a key step in regulating other cellular processes and viral replication.
Mastering XSLT (2002)

From the Publisher:
XSLT's capabilities and uses are so extensive that many books get bogged down in the complex W3C specification and provide little in the way of practical, real-world instruction. Mastering XSLT is a different kind of book. Here you'll find quick, efficient introductions to the key concepts, written for people who know their XML, and then detailed instructions that will help you meet your immediate document-transformation challenges and move on to new ones. It's the most effective way to get what you need out of XSLT, whether your application is simple or complex, conventional or ground-breaking.