gebhart/patrepeval huggingface dataset:
<i>age</i> Mutants of Arabidopsis Exhibit Altered Auxin-Regulated Gene Expression (1998)

An Arabidopsis transgenic line was constructed expressing beta-glucuronidase (GUS) via the auxin-responsive domains (AuxRDs) A and B (BA-GUS) of the PS-IAA4/5 gene in an indoleacetic acid (IAA)-dependent fashion. GUS expression was preferentially enhanced in the root elongation zone after treatment of young seedlings with 10(-7) M IAA. Expression of the BA-GUS gene in the axr1, axr4, and aux1 mutants required 10- to 100-fold higher auxin concentration than that in the wild-type background. GUS expression was nil in the axr 2 and axr 3 mutants. The transgene was used to isolate mutants exhibiting altered auxin-responsive gene expression (age). Two mutants, age1 and age2, were isolated and characterized. age1 showed enhanced sensitivity to IAA, with strong GUS expression localized in the root elongation zone in the presence of 10(-8) M IAA. In contrast, age2 exhibited ectopic GUS expression associated with the root vascular tissue, even in the absence of exogenous IAA. Morphological and molecular analyses indicated that the age1 and age2 alleles are involved in the regulation of gene expression in response to IAA.
Processing of high performance composites based on peek by aqueous suspension prepregging (2010)

The use of polyamic acid (PAA) precursor as interphase in polymer composites is one of the many applications of polyimides (PIs). In this work, composites based on poly(ether-ether-ketone) (PEEK) and carbon fibers were prepared using two manufacturing techniques for thermoplastic composites: hot compression molding, and aqueous polymeric suspension prepregging using PIs as interphase. Two PAAs were synthesized and used as interphases: 3,3'-4,4'-benzophenonetetracarboxylic dianhydride/oxydianiline (BTDA/ODA) and pyromellitic dianhydride/oxydianiline (PMDA/ODA). The PAA/PI systems were analyzed by differential scanning calorimetry (DSC), thermogravimetry (TGA), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). Results from these analyses confirmed the synthesis of these compounds. Aqueous polymeric suspension prepregging was more efficient than hot compression molding when the PMDA/ODA PAA/PI interphase was used; also, the interlaminar shear strength of composites produced using this technique was 14.5% higher than the one produced using hot compression molding.
Alpha-adrenergic receptor antagonists in older patients with benign prostatic hyperplasia: issues and potential complications. (2008)

Benign prostatic hyperplasia (BPH) is highly prevalent in men older than 50 years and is associated with a range of lower urinary tract symptoms that may have a negative impact on patient quality of life. Alpha(1)-adrenergic receptor antagonists are the first-line of pharmacologic management for lower urinary tract symptoms associated with BPH. However, many patients take multiple medications that may exacerbate age-related orthostatic hypotension. Thus, clinicians should evaluate the treatment of these patients within the context of comorbidities. The present article discusses the role of non-subtype-selective and subtype-selective alpha(1)-adrenergic receptor antagonists in the clinical management of BPH. Safety and tolerability for both non-subtype-selective and subtype-selective alpha(1)-adrenergic receptor antagonists for patients with BPH are also reviewed.
Composition and source contributions of air particulate matter pollution in a New Zealand suburban town (2011)

Wainuiomata, a suburban town located at the southern end of the North Island of New Zealand, is subject to air particulate matter pollution episodes during the winter. The results of fine (PM2.5) and coarse (PM2.5–10) particulate matter monitoring in Wainuiomata from July 2006–September 2008 are presented. Receptor modeling was used to determine the sources contributing to particulate matter pollution and mass contributions to ambient particle concentrations from emission sources were estimated. PM10 concentrations displayed a seasonal pattern, with peak concentrations occurring during the winter. The results demonstrate that marine aerosol and crustal matter sources were the primary contributors to the coarse particle fraction, while the fine particle fraction was dominated by biomass burning with smaller contributions from marine aerosol and secondary sulfate particles. Arsenic was found to be present in the fine particle fraction and was associated with biomass burning, suggesting the use of copper chrome arsenate treated timber for domestic heating. Analysis of seasonal differences revealed that biomass burning was largely responsible for fine particle pollution episodes during the winter. Marine aerosol featured significantly as a PM10 source all year due to New Zealand’s remote oceanic location.
A fully orthogonal system for protein synthesis in bacterial cells (2020)

Ribosome engineering is a powerful approach for expanding the catalytic potential of the protein synthesis apparatus. Due to the potential detriment the properties of the engineered ribosome may have on the cell, the designer ribosome needs to be functionally isolated from the translation machinery synthesizing cellular proteins. One solution to this problem was offered by Ribo-T, an engineered ribosome with tethered subunits which, while producing a desired protein, could be excluded from general translation. Here, we provide a conceptually different design of a cell with two orthogonal protein synthesis systems, where Ribo-T produces the proteome, while the dissociable ribosome is committed to the translation of a specific mRNA. The utility of this system is illustrated by generating a comprehensive collection of mutants with alterations at every rRNA nucleotide of the peptidyl transferase center and isolating gain-of-function variants that enable the ribosome to overcome the translation termination blockage imposed by an arrest peptide.
Breast Cancer Cell Lines Grown In Vivo: What Goes in Isn’t Always the Same as What Comes Out (2004)

Human cell lines constitute powerful model systems for the in vitro and in vivo analysis of cancer. Cancer lines that are invasive in culture often form tumors and metastases in immune deficient mice. It is generally assumed that, in such cases, the principal population of cancer cells in culture corresponds to the tumor-forming cells in vivo. Here, we high-light a recent functional proteomics investigation that suggests the contrary. In this study, cells derived from orthotopic xenograft tumors formed by the invasive breast cancer line MDA-MB-231 were found to exhibit profound differences in their enzyme activity profiles and increased tumor growth rates and metastasis when compared to the parental line. These findings suggest that the in vivo microenvironment of the mouse mammary fat pad cultivates the growth of human breast cancer cells with elevated tumorigenic properties. Characterization of the unique molecular properties of these tumor-forming cells may reveal new strategies for the diagnosis and treatment of breast cancer.
Secure names for bit-strings (1997)

Article Free Access Share on Secure names for bit-strings Authors: Stuart Haber Surety Technologies, 1 Main Street, Chatham, N.J. Surety Technologies, 1 Main Street, Chatham, N.J.View Profile , W. Scott Stornetta Surety Technologies, 1 Main Street, Chatham, N.J. Surety Technologies, 1 Main Street, Chatham, N.J.View Profile Authors Info & Claims CCS '97: Proceedings of the 4th ACM conference on Computer and communications securityApril 1997 Pages 28–35https://doi.org/10.1145/266420.266430Online:01 April 1997Publication History 56citation1,281DownloadsMetricsTotal Citations56Total Downloads1,281Last 12 Months176Last 6 weeks19 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Adaptation of the distal convoluted tubule of the rat. Structural and functional effects of dietary salt intake and chronic diuretic infusion. (1989)

We studied the effects of dietary NaCl intake on the renal distal tubule by feeding rats high or low NaCl chow or by chronically infusing furosemide. Furosemide-treated animals were offered saline as drinking fluid to replace urinary losses. Effects of naCl intake were evaluated using free-flow micropuncture, in vivo microperfusion, and morphometric techniques. Dietary NaCl restriction did not affect NaCl delivery to the early distal tubule but markedly increased the capacity of the distal convoluted tubule to transport Na and Cl. Chronic furosemide infusion increased NaCl delivery to the early distal tubule and also increased the rates of Na and Cl transport above the rates observed in low NaCl diet rats. When compared with high NaCl intake alone, chronic furosemide infusion with saline ingestion increased the fractional volume of distal convoluted tubule cells by nearly 100%, whereas dietary NaCl restriction had no effect. The results are consistent with the hypotheses that (a) chronic NaCl restriction increases the transport ability of the distal convoluted tubule independent of changes in tubule structure, (b) high rates of ion delivery to the distal nephron cause tubule hypertrophy, and (c) tubule hypertrophy is associated with increases in ion transport capacity. They indicate that the distal tubule adapts functionally and structurally to perturbations in dietary Na and Cl intake.
Experimental Toxoplasmosis and Vaccine Tests in Aotus Monkeys (1991)

We studied Aotus lemurinus, Panamanian night monkeys, for susceptibility to Toxoplasma infection and for their capacity to develop immunity using either sufadiazine prophylaxis or the non-persistent ts-4 vaccine. The animals were highly susceptible to infection with a mouse pathogenic (T265) and a mouse nonpathogenic (T163) Toxoplasma isolate. A calculated single bradyzoite by mouth gave rise to infection which was fatal in nine to 12 days. Chemoprophylaxis with 60-300 of sulfadiazine mg per day for up to 40 days protected the animals; however this was followed by fatal reactivation of infection between 11 and 70 days after treatment was stopped. Vaccination was carried out in two or three doses subcutaneously. Challenge was performed in 26 animals using both Toxoplasma isolates. Five monkeys (19%) survived for over a year, 10 died after a prolonged illness, and 11 died as rapidly as the seven controls. Safety tests showed the vaccine to be nonpathogenic in 111 adults except for slight fever and local inflammation, although one of four juveniles died from disseminated infection. Vaccination of 25 pregnant monkeys was non-pathogenic; however two of 25 fetuses were aborted, one of which was infected and one newborn had microphthalmia, retinitis and a cataract; four of the offspring were not tested. When six lactating monkeys were vaccinated, Toxoplasma was not transmitted to the infants. The high susceptibility to Toxoplasma and the low immunizability was circumstantially attributed to absence of exposure and lack of selection by Toxoplasma of these arboreal monkeys even though about 50% of terrestrial animals from the same area were infected.(ABSTRACT TRUNCATED AT 250 WORDS)
A Regulatory Gene of Phenylalanine Biosynthesis ( <i>pheR</i> ) in <i>Salmonella typhimurium</i> (1973)

4-Fluorophenylalanine-resistant mutants of Salmonella typhimurium were isolated in which synthesis of chorismate mutase P-prephenate dehydratase (specified by pheA) was highly elevated. Transduction analysis showed that the mutation affecting pheA activity was not linked to pheA, and conjugation and merodiploid analysis indicated that it was in the 95- to 100-min region of the Salmonella chromosome. Evidence is presented for the hypothesis that the mutation responsible for constitutivity of chorismate mutase P-prephenate dehydratase occurred in pheR, a gene specifying a cytoplasmic product that affected pheA. pheR mutants were found to carry a second mutation, tyrO. The tyrO mutation acts cis to cause increased levels of the tyrosine biosynthetic enzymes 3-deoxy-d-arabinoheptulosonate 7-phosphate synthetase (tyr) and prephenate dehydrogenase, but it has no effect on regulation of pheA.
Donor-Ligand-Substituted Cyclopentadienylchromium(III) Complexes:  A New Class of Alkene Polymerization Catalyst. 1. Amino-Substituted Systems (2000)

Me2NC2H4C5Me4Li reacts with Cr(THF)3Cl3 to give (η1:η5-Me2NC2H4C5Me4)CrCl2, in which the complexation of the N-donor atom to the metal atom has been confirmed by X-ray crystallography. A series of related compounds, e.g. (η1:η5-cyclo-C4H8NSiMe2OSiMe2C5H4)CrCl2, has been prepared by varying the substituents on the organic ligand. Further reaction with organomagnesium reagents leads to formation of the corresponding dialkyl−Cr complexes. Related species have been prepared containing imine-, alkoxy-, and alkylthio-substituted cyclopentadienyl groups as well as the C-donor ligand tetramethylimidazol-2-ylidene. Treatment of these compounds with methylalumoxane (MAO) leads to the formation of highly active catalysts for the oligomerization, polymerization, and copolymerization of ethylene.
Accurate Measurement of Density of Colloidal Latex Particles by Sedimentation Field-Flow Fractionation (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAccurate Measurement of Density of Colloidal Latex Particles by Sedimentation Field-Flow FractionationJ. Calvin Giddings and John HoCite this: Langmuir 1995, 11, 7, 2399–2404Publication Date (Print):July 1, 1995Publication History Published online1 May 2002Published inissue 1 July 1995https://pubs.acs.org/doi/10.1021/la00007a014https://doi.org/10.1021/la00007a014research-articleACS PublicationsRequest reuse permissionsArticle Views378Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Structural Basis for SARM1 Inhibition, and Activation Under Energetic Stress (2020)

Abstract SARM1 is a central executor of axonal degeneration ( 1 ). Mechanistically, SARM1 contains NADase activity, which, in response to nerve injury, depletes the key cellular metabolite, NAD+ ( 2–5 ). Interestingly, SARM1 knockout mouse models do not present any apparent physiological impairment. Yet, the lack of SARM1 protects against various neuropathies ( 6, 7 ), thereby highlighting SARM1 as a likely safe and effective drug target ( 8 ). However, the absence of a SARM1 structure, in its active or inhibited form, makes it impossible to understand the molecular basis of SARM1 inhibition, and its activation under stress conditions. In this study we present two cryo-EM maps of SARM1 (at 2.6 Å and 2.9 Å resolution). We show that the inhibited SARM1 homo-octamer assumes a packed conformation with well-ordered inner and peripheral rings. Here the catalytic TIR domains are held apart from each other and are unable to form dimers, which is a prerequisite for NADase activity. More importantly, after screening several cellular metabolites we discovered that the inactive conformation is stabilized by the binding of SARM1’s own substrate: NAD+. The NAD+ inhibitory allosteric site is located away from the NAD+ catalytic site of the TIR domain. Site-directed mutagenesis of the allosteric site leads to constitutive active SARM1. Based on our data we propose that a reduction of cellular NAD+ concentrations (an early indication of disease-associated and age-related neurodegeneration ( 9 )) disassemble SARM1’s peripheral ring, which allows NADase activity. This leads to an energetic catastrophe and eventually cell death. The discovery of the allosteric inhibitory site opens the door for the development of effective drugs that will prevent SARM1 activation, rather than compete for binding to the NADase catalytic site. Brief description It is not known how NAD+ depletion brings about neurodegeneration. Here, we show that the intrinsic NADase activity of SARM1 is allosterically inhibited by physiological concentrations of NAD+. NAD+ stabilizes a compact, auto-inhibited conformation of the SARM1 octamer. Once NAD+ levels are depleted, the allosteric inhibition is released, enabling SARM1’s NADase activity, which eventually leads to energetic catastrophe and cell death.
Architecture definition for small satellite personal communications systems (1993)

The paper deals with a satellite system, based on a low Earth orbit constellation, offering an optimum system architecture based on small simple (transparent or purely regenerative) satellites. All major critical aspects relevant to the space segment and to the ground segment are outlined, including orbital constellation characteristics and possible payload architecture. Furthermore, it addresses the major network aspects, focusing on the compatibility of the proposed satellite system with the existing or planned terrestrial infrastructure for personal communications. In particular, the main network requirements, in terms of functionalities, topology and architecture are dealt with. >
Design-Time Optimization of Post-Silicon Tuned Circuits Using Adaptive Body Bias (2008)

Adaptive body biasing is a powerful technique that allows post-silicon tuning of individual manufactured dies such that each die optimally meets the delay and power constraints. Assigning individual bias control to each gate leads to severe overhead, rendering the method impractical. However, assigning a single bias control to all gates in the circuit prevents the method from compensating for intra-die variation and greatly reduces its effectiveness. In this paper, we propose a new variability-aware method that clusters gates at design time into a handful of carefully chosen independent body-bias groups, which are then individually tuned post-silicon for each die. We show that this allows us to obtain near-optimal performance and power characteristics with minimal overhead. For each gate, we generate the probability distribution of its post-silicon ideal body bias voltage using an efficient sampling method. We then use these distributions and their correlations to drive a statistically aware clustering technique. We study the physical design constraints and show how the area and wirelength overhead can be significantly limited using the proposed method. Compared with a fixed design-time based dual threshold voltage assignment method, we improve leakage power by 38%-68% while simultaneously reducing the standard deviation of delay by two to nine times.
Temperature Gradient Gel Electrophoresis (TGGE) for the Detection of Polymorphic DNA and RNA (2003)

The potency of gel electrophoresis to analyze conformational transitions of nucleic acids has opened up a new field of applications. Helix coil transitions and, at a refined level, minor variations because of mutations and mismatches, may be studied experimentally by solvent -gradient and temperature-gradient gel electrophoresis. The latter method is the subject of this chapter.
Achieving transparency with plasmonic and metamaterial coatings (2005)

The possibility of using plasmonic and metamaterial covers to drastically reduce the total scattering cross section of spherical and cylindrical objects is discussed. While it is intuitively expected that increasing the physical size of an object may lead to an increase in its overall scattering cross section, here we see how a proper design of these lossless metamaterial covers near their plasma resonance may induce a dramatic drop in the scattering cross section, making these objects nearly "invisible" or "transparent" to an outside observer--a phenomenon with obvious applications for low-observability and noninvasive probe design. Physical insights into this phenomenon and some numerical results are provided.
Endometriosis of the inguinal region: Magnetic resonance imaging (MRI) findings (2007)

A case of endometrioma of the right inguinal canal region, diagnosed preoperatively, is presented. The diagnosis was made on the basis of cyclical symptoms relating to menstrual periods, in combination with demonstration of blood products within an enhancing focal lesion in the inguinal region with magnetic resonance imaging. The case presented here is unique, as it is the first case, to our knowledge, of an endometriotic lesion in the inguinal canal to demonstrate the characteristic 'shading sign' at magnetic resonance imaging.
Rapid thermal chemical vapor deposition of in-situ nitrogen-doped poly-silicon for dual gate CMOS (2002)

A novel gate structure with excellent electrical properties and reliability has been fabricated by in-situ rapid thermal multiprocessing. Gate oxide was grown first by low pressure rapid thermal oxidation in N/sub 2/O, followed by sequential rapid thermal chemical vapor deposition (RTCVD) of an ultrathin layer (6 nm) of nitrogen-doped polysilicon and then undoped polysilicon. Results show the suppression of boron penetration and high device reliability.
Compressed Air Work (1977)

American Occupational Medical Association 62nd Annual Meeting: April 25–28, 1977 Boston, Mass: Abstracts of Scientific Presentations: 1: 30 to 5: 00 p.m. CONCURRENT SESSION No. 3 Republic: PDF Only
Design and implementation of the "G2" PowerPC/sup TM/ 603e-embedded microprocessor core (2002)

New and emerging demands in the embedded market has precipitated in the development of the "G2" PowerPC 603e microprocessor into a reusable hard core. The demands and requirements of an embedded core microprocessor require significant changes in the physical design, verification and test strategies. Reuse and standardization in design data and test data present new challenges for an existing family of microprocessors.
Li-Ion Capacity Enhancement in Composite Blends of LiCoO[sub 2] and Li[sub 2]RuO[sub 3] (2005)

The specific capacity and energy of batteries with electrodes can be improved by the addition of to the cathodes, particularly at high rates. is a standard active material used in Li-ion batteries, and is a highly stable Li-insertion compound with high electronic and Li-ion conductivity. Batteries with and cathodes are compared to those with blends of the two active materials when discharged from at C/5 and 2C rates. The Li-ion batteries with a blend have a disproportionate increase of 25 and 36%, respectively, in their expected specific capacity and energy relative to when the batteries are charged and discharged at a 2C rate. X-ray diffraction of the electrodes at a high state of charge confirms that the and phases remain distinct in the electrodes. Analysis of the discharge curves in combination with electrochemical impedance spectroscopy shows that lowers the electrical resistance of the electrodes when combined in parallel with the . This simple approach may be used to improve the capacity of Li-ion batteries for high rate applications.