gebhart/patrepeval huggingface dataset:
Cryopreservation and Thawing of Mammalian Cells (2014)

Abstract Cryopreservation is the process by which intact living cells are preserved at cryogenic temperatures in liquid nitrogen. Freezing cells allow them to be stored, often for years, in a state where their normal metabolic activity is suspended in order to protect them from damage due to chemical reactivity and time. The cells can then be thawed and resuscitated as needed. Cells are cryopreserved in order to guard against genetic drift in continuous cell lines, or against transformation or differentiation in noncontinuous cell lines, such as stem cells and primary cells. In the case of primary cells that are isolated directly from the tissue of interest, they have a finite ability to divide; therefore, cryopreservation is necessary to preserve their unique characteristics for future experiments. Optimal cryopreservation of cells relies on proper freezing and thawing methods. To be protected from structural damage during the freeze‐thaw process, mammalian cells are frozen in the presence of cryoprotectant. Post‐thaw viability assays are then conducted to measure the success of the cryopreservation techniques by calculating the percentage of frozen cells that are alive and able to recover normal function once thawed. Key Concepts: Cryopreservation is used to enable long‐term storage, preserve current genetic state, prevent transformation or differentiation during subculture and provide a back‐up stock in case of infection/contamination during culture. A controlled rate of freezing and rapid thawing is necessary for optimal cryopreservation and recovery. Care must be taken to minimize transient warming events during transfer and storage, as it impacts viability and recovery. Active thawing results in higher cell viability and recovery than passive thawing. Timing is critical to all stages of the cryopreservation process. All materials should be ready before beginning the procedure, and steps should be taken to ensure each sample is handled with minimal delay. Method standardisation and quality control are necessary to maintain sample integrity and reproducibility.
A MICROFLUIDIC DEVICE TO MIMIC THE BONE MARROW MICROENVIRONMENT: REAL-TIME OBSERVATION OF THE LEUKEMIC CELL BEHAVIOR (2011)

We investigated the possibility of mimicking the bone marrow (BM) microenvironment using a microfluidic device. We succeeded in observing the differentiation into osteoclasts (OCs) and the migration of leukemic cells into the mimicked BM microenvironment. We confirmed that the BM microenvironment was mimicked in vitro successfully. These findings suggest that this attractive device can contribute to medical research.
High-order numerical simulation and modelling of the interaction of energetic and inert materials (2007)

We present an integrated algorithm on a Eulerian grid, for multimaterial simulations of energetic and inert materials modelled by non-ideal equations of state. We employ high-resolution shock capturing numerical algorithms for each material inside its domain and use an overlap domain method across the interface, maintained by a recently developed, hybrid, level-set algorithm. For applications to condensed explosives we implement a non-ideal, wide-ranging equation of state and reaction rate law. For inert materials, like plastic, metal, water, etc., we implement a (linear in the pressure) Mie–Grüneisen, (U p −U s ), equation of state. We present a series of verifications of the integrated multimaterial code and show validations against experiment. We show examples of simulations of various experiments associated with real or planned experiments, some of which contain energetic materials (specifically the condensed explosives PBX-9502 and PBX-9501).
Spiral Tilings with C-curves Using Combinatorics to Augment Tradition (2005)

Throughout the history of ornament artisans from many cultures have composed patterns utilizing spirals. Many of the compositions made by these artisans can be understood as the substitutions of C-curves or Scurves or a combination of C-curves and S-curves for the straight lines of a monohedral or polyhedral tiling [1]. The subject of this paper is the subset of these tilings composed with C-curves. Those known and used by traditional craftsmen are shown below in Figures 1 and 2 [2]. Artisans can apply and have applied an enormous variety of styles using these three tilings. An example of this variety can be seen by observing the expression of the same tiling, (4.4.4.4), in two different styles, a simple form in Figure 1a and a Celtic style in Figure 2a. These underlying tilings are part of a set of tilings with regular vertices commonly known as Laves tilings [3]. This paper identifies the complete set of tiles and examines some properties of the tilings that can be made with them. This greatly augments the number of underlying tilings available for use by artisans from three to an infinite number of tilings [4].
Boron and soda ash developments in the Middle East (2013)

Numan Kalbitemiz Bodur, Eti Mine's strategy development director, told delegates that the company, which had a production capacity of 570,000 tpa in 2000, and now has a capacity of 2.13m tpa, plans to expand to 5.5m tpa in the next 10 years.

Eti's strategy is to lead the development of the market by finding new boron products and uses, rather than waiting for the growth of the boron market, [Bodur] said.

In comparison, the MENA region produced 4% of soda ash in 2012, and China produced 43% of global soda ash, meaning that while the MENA region is a net importer China is a net exporter. North America produced 22% of global soda ash
Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– embryos at 12.5 to 14.5 days of gestation (2004)

The lipolysis stimulated receptor (LSR) recognizes apolipoprotein B/E-containing lipoproteins in the presence of free fatty acids, and is thought to be involved in the clearance of triglyceride-rich lipoproteins (TRL). The distribution of LSR in mice was studied by Northern blots, quantitative PCR and immunofluorescence. In the adult, LSR mRNA was detectable in all tissues tested except muscle and heart, and was abundant in liver, lung, intestine, kidney, ovaries and testes. During embryogenesis, LSR mRNA was detectable at 7.5 days post-coitum (E7) and increased up to E17 in parallel to prothrombin, a liver marker. In adult liver, immunofluorescence experiments showed a staining at the periphery of hepatocytes as well as in fetal liver at E12 and E15. These results are in agreement with the assumption that LSR is a plasma membrane receptor involved in the clearance of lipoproteins by liver, and suggest a possible role in steroidogenic organs, lung, intestine and kidney). To explore the role of LSR in vivo, the LSR gene was inactivated in 129/Ola ES cells by removing a gene segment containing exons 2-5, and 129/Ola-C57BL/6 mice bearing the deletion were produced. Although heterozygotes appeared normal, LSR homozygotes were not viable, with the exception of three males, while the total progeny of genotyped wild-type and heterozygote pups was 345. Mortality of the homozygote embryos was observed between days 12.5 and 15.5 of gestation, a time at which their liver was much smaller than that of their littermates, indicating that the expression of LSR is critical for liver and embryonic development.
Simple and Effective 3D Recognition of Domoic Acid Using a Molecularly Imprinted Polymer (2007)

We have developed a simple and effective molecular imprinting technique to target compounds with flexible structure. Domoic acid (DA), an amnesic shellfish poison, was used as the target compound while many acidic compounds (mono-, di-, and tricarboxylic acids) were used as template molecules for molecularly imprinted polymers (MIPs). Evaluation of selective recognition abilities using liquid chromatography revealed that the highest selective recognition ability for DA was found when pentane-1,3,5-tricarboxylic acid (1,3,5-PeTA) was used as the template. Computer modeling studies of the DA structure suggested that the observed selective recognition depended on the structural changes in DA at the recognition site of the MIPs as well as spatial distance between the COOH groups in DA and 1,3,5-PeTA. Using the 1,3,5-PeTA-MIP, we could easily purify DA from blue mussel extracts by solid-phase extraction.
A Partitioning Technique for a Waveform Relaxation Method Using Eigenvectors in the Transient Stability Analysis of Power Systems (2014)

It is of paramount importance that power system operators be able to assess transient stability in order to realize a reliable and stable power supply. Transient stability analysis can be formulated as a large-scale system of differential and algebraic equations (DAE). However, as power systems are becoming larger and more complex, it is becoming difficult to solve DAE in a practical amount of time for system operations. Parallel computing based on the waveform relaxation method is an effective solution to achieve faster calculations for transient stability analysis. To enhance the performance of the waveform relaxation method, a proper partitioning of the original problem is essential. Although various partitioning approaches have been used, those approaches might not be effective when analyzing a weakly damped low-frequency oscillation. In particular, in the Japanese 60-Hz power system, this oscillation becomes an important problem. To resolve this issue, in this paper we have developed a new partitioning method that is better suited to analyzing a weakly damped low-frequency oscillation based on eigenvalue analysis. Specifically, effective partitioning can be automatically determined by the proposed index, which can evaluate the validity of the partitioning. The proposed method was tested using the Japanese standards of the IEEJ WEST10 system model and the WEST30 system model.
Inhibition of interleukin‐12 expression by <i>α</i>‐thrombin in human peripheral blood mononuclear cells: a potential mechanism for modulating Th1/Th2 responses (2003)

In addition to its central role in blood coagulation and hemostasis, human alpha-thrombin is a powerful regulator of inflammatory responses and is known to affect cell-mediated immunity. Interleukin (IL)-12 is a strong promoter of the development of Th1-type lymphocytes and its downregulation implies a positive feedback mechanism for development of Th2 responses. We have previously shown that thrombin enhances the release of IL-6, a Th2-related cytokine, in human peripheral blood mononuclear cells (PBMC). Here we show that thrombin downregulates IL-12 production at both protein and mRNA levels in human PBMC. The inhibition of IL-12 production was accompanied by an enhanced release of IL-10, which inhibits Th1-related processes and promotes Th2-type responses. The use of proteolytically inactive thrombin and of the specific thrombin receptor agonist peptide, SFLLRN, reveals that this downregulation is thrombin-specific and requires thrombin proteolytic activity. In addition, activation of coagulation inhibits IL-12 production in whole blood cultures, confirming the tight relationship between the coagulation pathway, where thrombin is a key enzyme, and inflammation. Decreased IL-12 production appears to be related also to IL-10 production, since the addition of an anti-IL-10 monoclonal antibody to thrombin-treated PBMC resulted in a partial restoration of IL-12 production. In conclusion, the observation that thrombin significantly affects the production of IL-12, as well as of IL-10, implies a concerted role orchestrated by thrombin in PBMC that could be crucial to effective immunity and inflammation.
Supercritical fluid extraction of alcohols from water (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSupercritical fluid extraction of alcohols from waterEsteban A. Brignole, Poul M. Andersen, and Aage FredenslundCite this: Ind. Eng. Chem. Res. 1987, 26, 2, 254–261Publication Date (Print):February 1, 1987Publication History Published online1 May 2002Published inissue 1 February 1987https://pubs.acs.org/doi/10.1021/ie00062a013https://doi.org/10.1021/ie00062a013research-articleACS PublicationsRequest reuse permissionsArticle Views491Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optimal expression of cloned NMDAR1/NMDAR2A heteromeric glutamate receptors: a biochemical characterization (1993)

The N-methyl-D-aspartate R1 (NMDAR1) and NMDAR2A subunits were expressed transiently either alone or in combination in human embryonic kidney (HEK) 293 cells. The biochemical and pharmacological properties of the cloned receptors were compared with those of adult rat brain NMDA receptors using both immunological methods with a newly developed anti-NMDAR2A-(1435-1445) antibody and [3H]MK801 radioligand binding activity. Anti-NMDAR2A-(1435-1445) antibodies recognized specifically four immunoreactive species with M(r)s of 180,000, 122,000, 97,000 and 54,000 in rat brain, but only a single band of M(r) 180,000 in HEK 293 cells singly transfected with plasmid pCISNMDAR2A. N-deglycosylation of HEK cell membranes yielded a 165,000-M(r) immunoreactive species, which is in agreement with the size predicted from the cDNA sequence for the mature NMDAR2A subunit. Co-expression of NMDAR1 and NMDAR2A subunits in HEK 293 cells resulted in cell death. Thus conditions were established for the optimum expression of heteromeric receptors in viable cells, including a requirement for DL-2-amino-5-phosphonopentanoic acid (AP5) in the culture medium post-transfection. Cells transfected with pCISNMDAR1 and pCISNMDAR2A combined yielded a 10-fold increase in the number of [3H]MK801 binding sites compared with single subunit expression. MK801 had similar affinity for the expressed receptors as for those found in adult rat and mouse brain. These results demonstrate that the NMDAR1 and NMDAR2A receptor subunits co-assemble to form a heteromeric complex with properties similar to those of the native receptors of adult mammalian forebrain. Furthermore, the conditions reported for maximal transient expression provide a basis for further structure-activity studies.
ChemInform Abstract: Novel Synthesis of Thieno(2,3‐c)pyrazoles and Thieno(2,3‐d)pyrimidines. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Thiadiazepine ring system (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThiadiazepine ring systemHeino A. LutsCite this: J. Org. Chem. 1968, 33, 12, 4528–4529Publication Date (Print):December 1, 1968Publication History Published online1 May 2002Published inissue 1 December 1968https://pubs.acs.org/doi/10.1021/jo01276a051https://doi.org/10.1021/jo01276a051research-articleACS PublicationsRequest reuse permissionsArticle Views115Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Reactivity of human leukocyte elastase and porcine pancreatic elastase toward peptide-4-nitroanilides containing model desmosine residues. Evidence that human leukocyte elastase is selective for crosslinked regions of elastin (1981)

Elastin contains a number of cross-linking amino acid residues such as desmosine and isodesmosine which are primarily hydrophobic in character, but have a positively charged pyridinium ring. These cross-linking residues are formed by the action of lysyl oxidase upon Lys residues in tropoelastin, a precursor of elastin. A series of tetrapeptide 4-nitroanilides which contain Lys and a series of modified lysine residues were synthesized. The modified lysine residues [epsilon-carbobenzyloxy (Z), epsilon-benzoyl (Bz), epsilon-benzimidoyl (Bim), and epsilon-2-picolinoyl (Pic)] have various characteristics of desmosine and isodesmosine residues, such as a positive charge, a hydrophobic aromatic ring, or a pyridine ring. The reactivity of the tetrapeptide 4-nitroanilides containing the model desmosine residues at P4, P3, or P2 with human leukocyte (HL) and porcine pancreatic (PP) elastase was measured at pH 7.5 and 25 degrees C. HL elastase exhibited high reactivity toward the substrates with P4 or P3 hydrophobic groups (Z, Bz, or Pic), and MeO-Suc-Lys(Pic)-Ala-Pro-Val-NA is 7 times more reactive than the previous best HL elastase substrate, MeO-Suc-Ala-Ala-Pro-Val-NA. The major change occurred in KM values. The substrates containing Lys residues were either nonreactive or poor. Except for two substrates with P2 hydrophobic residues (Bz and Pic), PP elastase was less reactive toward the substrates containing model desmosine residues than toward MeO-Suc-Ala-Ala-Pro-Val-NA. The data support the hypothesis that HL elastase cleaves elastin selectively ner cross-linking residues. The results also indicate that HL elastase binds tightly to these regions and would be poorly effective toward regions of elastin or tropoelastin which contain Lys residues.
The Effect of Regular Exercise on Cardiovascular Inflammation Markers in Elderly Korean Men (2005)

This study was performed to investigate the effect of regular aerobic exercise on the representative markers of cardiovascular inflammatory diseases. Biomarkers of cardiovascular disease were measured in 89 healthy elderly males (age range; 47–88) living in Seoul, Korea. Questionnaires were used to assess the status of the regular exercise participants. Various factors associated with chronic cardiovascular diseases, including total cholesterol, triglyceride, phospholipid, platelet, and C-reactive protein were lowered in regular exercise participants (p<0.05). In contrast, serum albumin concentration was higher in the exercised group compared with a non-exercised group (p<0.05). Therefore it was concluded that participation in regular exercise reduces cardiovascular inflammation and offers protection against chronic cardiovascular disease in elderly Korean men.
Audio Packet Loss Concealment in a Combined MDCT-MDST Domain (2007)

Audio streaming applications have become very popular in recent years, owing to their low cost and convenience. However, during network congestions, data packets are often delayed or discarded, creating an annoying gap in the streamed media. This letter presents a new approach to audio packet loss concealment designed for MPEG-audio streaming applications. In a previous work, we introduced a receiver-based concealment algorithm based on applying the gapped-data amplitude and phase estimation (GAPES) interpolation algorithm in the discrete short-time Fourier transform (DSTFT) complex domain and obtained better results compared to past methods. The current approach applies the same algorithm on a different complex domain, formed from combining the modified discrete cosine transform (MDCT) domain as its real part and the modified discrete sine transform (MDST) domain as the imaginary part. The new approach significantly reduces the complexity demands while maintaining similar high-quality results.
Prevalence and Characterization of Non-O157 Shiga Toxin-Producing<i>Escherichia coli</i>Isolates from Commercial Ground Beef in the United States (2011)

Escherichia coli O157:H7 is a Shiga toxin (stx)-producing E. coli (STEC) strain that has been classified as an adulterant in U.S. beef. However, numerous other non-O157 STEC strains are associated with diseases of various severities and have become an increasing concern to the beef industry, regulatory officials, and the public. This study reports on the prevalence and characterization of non-O157 STEC in commercial ground beef samples (n = 4,133) obtained from numerous manufacturers across the United States over a period of 24 months. All samples were screened by DNA amplification for the presence of Shiga toxin genes, which were present in 1,006 (24.3%) of the samples. Then, culture isolation of an STEC isolate from all samples that contained stx(1) and/or stx(2) was attempted. Of the 1,006 positive ground beef samples screened for stx, 300 (7.3% of the total of 4,133) were confirmed to have at least one strain of STEC present by culture isolation. In total, 338 unique STEC isolates were recovered from the 300 samples that yielded an STEC isolate. All unique STEC isolates were serotyped and were characterized for the presence of known virulence factors. These included Shiga toxin subtypes, intimin subtypes, and accessory virulence factors related to adherence (saa, iha, lifA), toxicity (cnf, subA, astA), iron acquisition (chuA), and the presence of the large 60-MDa virulence plasmid (espP, etpD, toxB, katP, toxB). The isolates were also characterized by use of a pathogenicity molecular risk assessment (MRA; based on the presence of various O-island nle genes). Results of this characterization identified 10 STEC isolates (0.24% of the 4,133 total) that may be considered a significant food safety threat, defined by the presence of eae, subA, and nle genes.
Comparison of Parabolic and Gaussian Lateral Cosine Modulations in Ultrasound Imaging, Displacement Vector Measurement, and Elasticity Measurement (2008)

Previously, we proposed to set ultrasound (US) beam-forming parameters in order to realize the required point spread function (PSF) on the basis of optimization theory. In this report, for high quality-lateral cosine modulation (LCM) US imaging and measurements of accurate displacement vector and elasticity (i.e., strain tensor, shear modulus), after briefly reviewing our trials that broke away from the use of the Fraunhofer approximation to determine the apodization function, images and measurements obtained for an agar phantom are shown. In this study, the previously reported lateral parabolic modulation (PAM) and lateral Gaussian envelope cosine modulation (LGECM) are carried out. Comparisons of the spatial resolutions of the US images obtained and the measurement accuracies of the displacement vectors and elasticity measurements obtained using our previously developed multidimensional autocorrelation and Doppler methods (i.e., MAM and MDM) are also made. The development of next-generation US imaging systems has already begun.