gebhart/patrepeval huggingface dataset:
Oxygen-derived free radicals stimulate osteoclastic bone resorption in rodent bone in vitro and in vivo. (1990)

The mechanisms by which bone resorbing osteoclasts form and are activated by hormones are poorly understood. We show here that the generation of oxygen-derived free radicals in cultured bone is associated with the formation of new osteoclasts and enhanced bone resorption, identical to the effects seen when bones are treated with hormones such as parathyroid hormone (PTH) and interleukin 1 (IL-1). When free oxygen radicals were generated adjacent to bone surfaces in vivo, osteoclasts were also formed. PTH and IL-1-stimulated bone resorption was inhibited by both natural and recombinant superoxide dismutase, an enzyme that depletes tissues of superoxide anions. We used the marker nitroblue tetrazolium (NBT) to identify the cells that were responsible for free radical production in resorbing bones. NBT staining was detected only in osteoclasts in cultures of resorbing bones. NBT staining in osteoclasts was decreased in bones coincubated with calcitonin, an inhibitor of bone resorption. We also found that isolated avian osteoclasts stained positively for NBT. NBT staining in isolated osteoclasts was increased when the cells were incubated with bone particles, to which they attach. We confirmed the formation of superoxide anion in isolated avian osteoclasts using ferricytochrome c reduction as a method of detection. The reduction of ferricytochrome c in isolated osteoclasts was inhibited by superoxide dismutase. Our results suggest that oxygen-derived free radicals, and particularly the superoxide anion, are intermediaries in the formation and activation of osteoclasts.
THE REDUCTION OF CYSTINE IN LIQUID AMMONIA BY METALLIC SODIUM<sup>1</sup> (1930)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE REDUCTION OF CYSTINE IN LIQUID AMMONIA BY METALLIC SODIUM1Vincent du Vigneaud, L. F. Audrieth, and H. S. LoringCite this: J. Am. Chem. Soc. 1930, 52, 11, 4500–4504Publication Date (Print):November 1, 1930Publication History Published online1 May 2002Published inissue 1 November 1930https://pubs.acs.org/doi/10.1021/ja01374a047https://doi.org/10.1021/ja01374a047research-articleACS PublicationsRequest reuse permissionsArticle Views417Altmetric-Citations83LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Evaluation of Use and Quality of Public E-services among Enterprises (2012)

The paper deals with the quality evaluation of electronic services. The European Commission eGovernment benchmarking effort is reviewed and the current level of use of basic electronic services for enterprises is compared between EU 27 member countries and the Czech Republic. Then, the most frequently used services are evaluated among 452 enterprises in the Czech Republic. The evaluation was based on a method CBG (Communication between Business and Government) that has been developed at the Department of information technologies at the Faculty of Economic and Management at Czech University of Life Sciences (CULS) in Prague. Results of the survey are compared to prior surveys, Czech Statistical Office and Eurostat surveys. Pieces of knowledge introduced in this paper resulted from solution of an institutional research intention MSMT 6046070906 „Economics of resources of Czech agriculture and their efficient use in frame of multifunctional agri-food systems“.
Fertilization of Mixed Cheatgrass-Bluebunch Wheatgrass Stands (1966)

On both dense and sparse bluebunch wheatgrass stands, cheatgrass became dominant with increasing applications of ammonium sulfate. High and repeated fertilizer applications (80 lb N/A in 4 successive years) depressed bluebunch wheatgrass yield 50%.
Removal of Coxsackie and Bacterial Viruses in Water by Flocculation (1958)

Removal of Coxsackie and Bacterial Viruses in Water by Flocculation II. Removal of Coxsackie and Bacterial Viruses and the Native Bacteria in Raw Ohio River Water by Flocculation with Aluminum Sulfate and Ferric Chloride Shih L. Chang, Robert E. Stevenson, Alton R. Bryant, Richard L. Woodward, and Paul W. Kabler CopyRight https://doi.org/10.2105/AJPH.48.2.159 Published Online: August 29, 2011
Anaerobic survival of clinical isolates and laboratory strains of Neisseria gonorrhoea: use in transfer and storage (1982)

Eleven laboratory strains and 67 clinical isolates of Neisseria gonorrhoeae were tested for the ability to survive during anaerobic incubation. The survival of the laboratory strains was dependent on auxotype, temperature, and cell density on agar plates. For both the laboratory strains and the clinical isolates, anaerobic survival was better at lower temperatures. We concluded that anaerobic incubation, for as long as 7 days, is useful when transporting or storing N. gonorrhoeae.
Muscle as an Endocrine Organ: Focus on Muscle-Derived Interleukin-6 (2008)

Skeletal muscle has recently been identified as an endocrine organ. It has, therefore, been suggested that cytokines and other peptides that are produced, expressed, and released by muscle fibers and exert paracrine, autocrine, or endocrine effects should be classified as "myokines." Recent research demonstrates that skeletal muscles can produce and express cytokines belonging to distinctly different families. However, the first identified and most studied myokine is the gp130 receptor cytokine interleukin-6 (IL-6). IL-6 was discovered as a myokine because of the observation that it increases up to 100-fold in the circulation during physical exercise. Identification of IL-6 production by skeletal muscle during physical activity generated renewed interest in the metabolic role of IL-6 because it created a paradox. On one hand, IL-6 is markedly produced and released in the postexercise period when insulin action is enhanced but, on the other hand, IL-6 has been associated with obesity and reduced insulin action. This review focuses on the myokine IL-6, its regulation by exercise, its signaling pathways in skeletal muscle, and its role in metabolism in both health and disease.
An Investigation of the N-Arylsulfonylation of 2-Azetidinones (1997)

Abstract N-Arylsulfonylation of 2-azetidinones can lead to the diastereoselective formation of oligomerization products. However, a simple increase of arylsulfonyl chloride concentration minimized oligomerization and allowed preparation of 1-arylsulfonyl-2-azetidinones in good yield.
Aircraft Lightning Protection Handbook (1989)

This handbook will assist aircraft design, manufacturing, and certification organizations in protecting aircraft against the direct and indirect effects of lightning strikes, in compliance with Federal Aviation Regulations. It presents a comprehensive text to provide the essential information for the in-flight lightning protection of all types of fixed/rotary wing and powered lift aircraft of conventional, composite, and mixed construction and their electrical and fuel systems. The handbook contains chapters on the natural phenomenon of lightning, the interaction between the aircraft and the electrically charged atmosphere, the mechanism of the lightning strike, and the interaction with the airframe, wiring, and fuel system. Further chapters cover details of designing for optimum protection; the physics behind the voltages, currents, and electromagnetic fields developed by the strike; and shielding techniques and damage analysis. The handbook ends with discussion of test and analytical techniques for determining the adequacy of a given protection scheme. Keywords: Lightning protection, Fuel vapor ignition, Fuel system safety, Atmospheric electrical hazards, Lightning safety, Lightning simulation, Aircraft certification, Atmospheric electromagnetism, Induced currents & voltages, Electromagnetic shielding.
Structural Analysis of the Hemagglutinin from the Recent 2013 H7N9 Influenza Virus (2013)

In March 2013, the Chinese Center for Disease Control and Prevention reported human infections with an H7N9 influenza virus, and by 20 July 2013, the numbers of laboratory-confirmed cases had climbed to 134, including 43 fatalities and 127 hospitalizations. The newly emerging H7N9 viruses constitute an obvious public health concern because of the apparent severity of this outbreak. Here we focus on the hemagglutinins (HAs) of these viruses and assess their receptor binding phenotype in relation to previous HAs studied. Glycan microarray and kinetic analyses of recombinant A(H7N9) HAs were performed to compare the receptor binding profile of wild-type receptor binding site variants at position 217, a residue analogous to one of two positions known to switch avian to human receptor preference in H2N2 and H3N2 viruses. Two recombinant A(H7N9) HAs were structurally characterized, and a mutational study of the receptor binding site was performed to analyze important residues that can affect receptor preference and affinity. Results highlight a weak human receptor preference of the H7N9 HAs, suggesting that these viruses require further adaptation in order to adapt fully to humans.
Magnesium in stroke treatment (2002)

Magnesium is involved in multiple physiological processes that may be relevant to cerebral ischaemia, including antagonism of glutamate release, NMDA receptor blockade, calcium channel antagonism, and maintenance of cerebral blood flow. Systemically administered magnesium at doses that double physiological serum concentration significantly reduces infarct volume in animal models of stroke, with a window of up to six hours after onset and favourable dose-response characteristics when compared with previously tested neuroprotective agents. Small clinical trials have reported benefit, but results are not statistically significant in systematic review. A large ongoing trial (IMAGES) will report in 2003-4 and further trials are planned.
Quantum chemical studies of a model for peptide bond formation. 2. Role of amine catalyst in formation of formamide and water from ammonia and formic acid (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantum chemical studies of a model for peptide bond formation. 2. Role of amine catalyst in formation of formamide and water from ammonia and formic acidTetsuro Oie, Gilda H. Loew, Stanley K. Burt, and Robert D. MacElroyCite this: J. Am. Chem. Soc. 1983, 105, 8, 2221–2227Publication Date (Print):April 1, 1983Publication History Published online1 May 2002Published inissue 1 April 1983https://pubs.acs.org/doi/10.1021/ja00346a020https://doi.org/10.1021/ja00346a020research-articleACS PublicationsRequest reuse permissionsArticle Views184Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Rateless space-time coding (2005)

Rateless codes are good codes of infinite length that have the property that prefixes of such codes are themselves good codes. This makes them attractive for applications in which the channel quality is uncertain, where systems transmit as much of a codeword as necessary for decoding to be possible. In particular, rateless codes are potentially attractive for wireless communication. In a recent work, a rateless coding scheme was proposed for the AWGN channel, based on layering, repetition and random dithering. We extend this scheme to multiple-input single-output (MISO) Gaussian channels. We show that the rate loss associated with orthogonal design space-time codes may be alleviated by layering and dithering, very similar to the rateless approach for the AWGN channel. We then combine the two schemes and arrive at a close-to-capacity rateless code for MISO channels. The required complexity depends on the fraction of capacity that is targeted, is linear in the capacity of the channel and does not depend on the number of transmit antennas. Furthermore, the coding scheme uses only one base AWGN code
Object Recognition, Segmentation, and Classification of Mobile Laser Scanning Point Clouds: A State of the Art Review (2019)

Mobile Laser Scanning (MLS) is a versatile remote sensing technology based on Light Detection and Ranging (lidar) technology that has been utilized for a wide range of applications. Several previous reviews focused on applications or characteristics of these systems exist in the literature, however, reviews of the many innovative data processing strategies described in the literature have not been conducted in sufficient depth. To this end, we review and summarize the state of the art for MLS data processing approaches, including feature extraction, segmentation, object recognition, and classification. In this review, we first discuss the impact of the scene type to the development of an MLS data processing method. Then, where appropriate, we describe relevant generalized algorithms for feature extraction and segmentation that are applicable to and implemented in many processing approaches. The methods for object recognition and point cloud classification are further reviewed including both the general concepts as well as technical details. In addition, available benchmark datasets for object recognition and classification are summarized. Further, the current limitations and challenges that a significant portion of point cloud processing techniques face are discussed. This review concludes with our future outlook of the trends and opportunities of MLS data processing algorithms and applications.
Factors triggering type III secretion in Pseudomonas aeruginosa (2005)

The type III secretion system of Pseudomonas aeruginosa is tightly regulated by various environmental signals, such as low calcium and contact with the host cell. However, the exact signals triggering type III secretion are unknown. The present study describes the finding that secretion of P. aeruginosa type III effector molecules requires protein factors from serum and L broth, designated type III secretion factors (TSFs), in addition to the low-calcium environment. In the absence of TSF or calcium chelator EGTA, basal levels of type III effector molecules are accumulated intracellularly. Addition of TSF and EGTA together effectively triggers the secretion of pre-existing effector molecules in a short time, even before the active expression of type III genes; thus, active type III gene expression does not seem to be a prerequisite for type III secretion. A search for TSF molecules in serum and L broth resulted in the identification of albumin and casein as the functional TSF molecules. Although there is no clear sequence similarity between albumin and casein, both proteins are known to have a low-affinity, high-capacity calcium-binding property. Tests of well-studied calcium-binding proteins seemed to indicate that low-affinity calcium-binding proteins have TSF activity, although the requirement of low-affinity calcium-binding ability for the TSF activity is not clear. P. aeruginosa seems to have evolved a sensing mechanism to detect target cells for type III injection through host-derived proteins in combination with a low-calcium signal. Disruption of the bacterial ability to sense low calcium or TSF might be a valid avenue to the effective control of this bacterial pathogen.
Nox1-dependent Reactive Oxygen Generation Is Regulated by Rac1 (2006)

Rac1 has been implicated in the generation of reactive oxygen species (ROS) in several cell types, but the enzymatic origin of the ROS has not been proven. The present studies demonstrate that Nox1, a homolog of the phagocyte NADPH-oxidase component gp91(phox), is activated by Rac1. When Nox1 is co-expressed along with its regulatory subunits NOXO1 and NOXA1, significant ROS generation is seen. Herein, co-expression of constitutively active Rac1(G12V), but not wild-type Rac1, resulted in marked further stimulation of activity. Decreased Rac1 expression using small interfering RNA reduced Nox1-dependent ROS. CDC42(G12V) failed to increase activity, and small interfering RNA directed against CDC42 failed to decrease activity, pointing to specificity for Rac. TPR domain mutants of NOXA1 that interfere with Rac1 binding were ineffective in supporting Nox1-dependent ROS generation. Immunoprecipitation experiments demonstrated a complex containing Rac1(G12V), NOXO1, NOXA1, and Nox1. CDC42(G12V) could not substitute for Rac1(G12V) in such a complex. Nox1 formed a complex with Rac1(G12V) that was independent of NOXA1 and NOXO1, consistent with direct binding of Rac1(G12V) to Nox1. Rac1(G12V) interaction with NOXA1 was enhanced by Nox1 and NOXO1, suggesting cooperative binding. A model is presented comparing activation by regulatory subunits of Nox1 versus gp91(phox) (Nox2) in which Rac1 activation provides a major trigger that acutely activates Nox1-dependent ROS generation.
REVIEW (1994)

More effective measures to control and replace the dopaminergic deficit of Parkinson's disease are being actively sought. One basic problem is how the striatal dopamine loss should be replaced in order to mimic most accurately the physiological state. Animal electrophysiology indicates that the dopaminergic nigrostriatal pathway has a dual tonic and phasic action. Intermittent dopaminergic stimulation is associated with behavioural hyposensitivity both in animal models and in patients with Parkinson's disease. Continuous dopaminergic stimulation provides a tonic background and improves some clinical problems but is also associated with tolerance. None of the available pharmacological approaches can restore the dopamine deficiency of Parkinson's disease to physiological levels. Continuous dopaminergic stimulation for < 24 h, associated with small doses of levodopa or a short-acting dopamine agonist, appears to be the best, albeit imperfect, therapeutic approach until other, more efficacious remedies are developed.
Overexpression of acid ceramidase (ASAH1) protects retinal cells (ARPE19) from oxidative stress (2018)

Over 11 million people in the United States alone have some form of age-related macular degeneration (AMD). Oxidative stress, cell death, and the degeneration of retinal pigment epithelial (RPE) cells contribute to AMD pathology. Recent evidence suggests that ceramide (Cer), a cellular sphingolipid mediator that acts as a second messenger to induce apoptosis, might play a role in RPE cell death. The lysosomal breakdown of Cer by acid ceramidase [<i>N</i>-acylsphingosine amidohydrolase (ASAH)1] into sphingosine (Sph) is the major source for Sph 1-phosphate production, which has an opposing role to Cer and provides cytoprotection. Here, we investigated the role of Cer in human RPE-derived ARPE19 cells under hydrogen peroxide-induced oxidative stress, and show that Cer and hexosyl-Cer levels increase in the oxidatively stressed ARPE19 cells, which can be prevented by overexpression of lysosomal ASAH1. This study demonstrates that oxidative stress generates sphingolipid death mediators in retinal cells and that induction of ASAH1 could rescue retinal cells from oxidative stress by hydrolyzing excess Cers.
A bichromatic method for total bilirubin with a CentrifiChem 400. (1979)

We describe a method for the bichromatic determination of total bilirubin with a CentrifiChem 400 centrifugal analyzer with the Boehringer DPD kit. After reading the blank at 620 nm, we read the test at 550 nm. Because only one rotor is required, the analytical time for bilirubin estimations is halved. The proposed method is no more prone to interference by hemoglobin or turbidity than a conventional approach or a reference continuous-flow method and has performed well over several months of routine use.
Information Hiding Scheme for GIS Shapefile (2010)

In this paper, we present a watermarking scheme for the copyright protection of GIS vector map based on shapefile. We embed a watermark bit to the local mean perimeter of a suitable group of polygon data by changing all coordinates of vertices according to the constraint of the robustness and the invisibility. Experimental result shows that the proposed scheme has invisibility to users.
Microtubules are acetylated in domains that turn over slowly (1989)

Tubulin is subject to a post-translational acetylation reaction that is thought to be correlated with increased stability of the modified microtubules (MTs). We sought to test directly the stability of acetylated MTs by determining their specific rate of turnover. We used human fibroblasts, which contain a subset of MTs that display terminal and internal domains of acetylation. The turnover of acetylated domains was analysed by microinjecting cells with biotinylated brain tubulin and determining, by triple-label immunofluorescence, the progress of incorporation of biotinylated tubulin into acetylated and non-acetylated domains. Within two minutes after injection, biotinylated domains were contiguous with virtually all observed non-acetylated MT ends but were not contiguous with terminal acetylated domains, demonstrating that the former were growing while the latter were not. Ten minutes after injection, many MTs lacking acetylated domains had incorporated biotinylated subunits uniformly while most MTs containing acetylated domains remained unlabelled, indicating that non-acetylated MTs were turning over while most acetylated domains were not. One hour after injection, virtually all non-acetylated MTs were labelled with biotin whereas approximately half of the acetylated domains contained biotin, demonstrating that acetylated domains turned over much more slowly than the non-acetylated, bulk array. Non-acetylated MT regions flanking acetylated domains also lacked hapten, indicating that acetylation modified discrete regions along stable MTs. Sixteen hours after injection, cells that had not entered mitosis still retained acetylated domains that had not turned over (13% of all acetylated domains), indicating that acetylated domains can be extremely long-lived.(ABSTRACT TRUNCATED AT 250 WORDS)