gebhart/patrepeval huggingface dataset:
N<sup>1</sup>‐Methylnicotinamide Level in the Blood after Nicotinamide Loading as Further Evidence for Malignant Tumor Burden (1991)

Nicotinamide methyltransferase (Nmd CH3transferase) activity increased in the liver of mice after i.p. transplantation of Ehrlich ascites tumor (ascitic form), but not in the liver of mice with acute inflammation induced by the i.p. administration of D-galactosamine, and it rather showed a decrease together with necrosis after carbon tetrachloride administration. When Nmd CH3transferase activity of rat hepatocytes in primary culture was investigated with the addition of dexamethasone, epidermal growth factor, transforming growth factor-beta, tumor necrosis factor-alpha and N1-methylnicotinamide (1-CH3Nmd), changes in activity were not correlated with DNA synthesis, suggesting that the increase of this enzyme activity in the tumor host liver was not directly related to liver cell proliferation. Thus, in order to make use of the increase of this enzyme activity as a tumor burden marker, a procedure for its estimation by measuring the blood level of 1-CH3Nmd, a metabolite of Nmd produced by Nmd CH3transferase, was established. The 1-CH3Nmd level in the blood of mice bearing Ehrlich ascites tumor 4 h after s.c. loading of Nmd (500 mg/kg body weight) was closely correlated with this enzyme activity in the liver (r = 0.835, P less than 0.00001) from the early to the terminal stage of tumor development. Furthermore, similar correlations were seen in the animal groups bearing various other tumors, such as s.c. implanted Ehrlich ascites tumor (solid form) and i.p. implanted sarcoma S-180, hepatoma MH-134, Yoshida ascites sarcoma and leukemia L-1210, but not solid tumors such as Lewis lung carcinoma and melanoma B-16, although almost all of the animals bearing these tumors showed a higher enzyme activity than their control normal animals.
Gross-Merchantable Timber Volume Estimation Using an Airborne Lidar System (1986)

RÉSUMÉUne étude préliminaire visant à déterminer l'utilité d'un laser aéroporté comme moyen employé par les gestionnaires forestiers pour le calcul du volume forestier marchand brut a été effectuée près de la National Aeronautics and Space Administration (NASA) Goddard Space Flight Center, Wallops Flight Facility, au moyen du lidar océanographique aéroporté (LOA) de la NASA. Le volume forestier mesuré a été mis en régression avec la section efficace du lidar le long d'un profil de la forêt. On a constaté que ce profil de la section efficace du lidar constituait une variable très importante dans l'estimation du volume forestier marchand brut. La stratification des essences d'arbres a permis d'obtenir des résultats beaucoup plus satisfaisants, soit une valeur globale de R2 de 0,921, pour un niveau de confiance de la régression de 99 %.
The effects of mutational processes and selection on driver mutations across cancer types (2017)

ABSTRACT Epidemiological evidence has long associated environmental mutagens with increased cancer risk. However, links between specific mutation-causing processes and the acquisition of individual driver mutations have remained obscure. Here we have used public cancer sequencing data to infer the independent effects of mutation and selection on driver mutation complement. First, we detect associations between a range of mutational processes, including those linked to smoking, ageing, APOBEC and DNA mismatch repair (MMR) and the presence of key driver mutations across cancer types. Second, we quantify differential selection between well-known alternative driver mutations, including differences in selection between distinct mutant residues in the same gene. These results show that while mutational processes play a large role in determining which driver mutations are present in a cancer, the role of selection frequently dominates.
Empirical Observations and Mechanistic Insights on the First Boron-Containing Chiral Selector for LC and Supercritical Fluid Chromatography (2007)

Boromycin is a macrodiolide that exists as a hydrophobic Böeseken complex formed from boric acid and a chiral polyhydroxy macrocyclic ligand. It was covalently bonded to silica gel through a urea linkage to an attached d-valine ester. When evaluated as a chiral stationary phase, it shows pronounced enantioselectivity toward primary amine-containing racemates, separating 98% of those tested. The selectivity is most pronounced in the presence of organic solvents and supercritical CO2 + methanol. The enantioselective binding site and mechanistic factors are examined. Analytes can be complexed as either the free base or their protonated analogues, with the free base being more strongly associated with the chiral selector.
LPTV SUBSPACE ANALYSIS OF WIND TURBINES DATA (2014)

HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci-entific research documents, whether they are pub-lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et a ̀ la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Purification and enzymic characterization of the cytoplasmic pyrophosphatase from the thermoacidophilic archaebacterium <i>Thermoplasma acidophilum</i> (1992)

Cytoplasmic pyrophosphatase has been isolated from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme was purified to electrophoretic homogeneity by combining ion-exchange and affinity-chromatographic separations. This soluble pyrophosphatase probably consists of six identical subunits, since SDS/PAGE gave an estimate of about 22 kDa for a single subunit and size-exclusion chromatography under non-denaturing conditions indicates a molecular mass of 110 +/- 5 kDa. The two most prominent catalytic features of this enzyme are the absolute requirement for divalent cations for catalytic action, Mg2+ conferring the highest activity, and the pronounced specificity for PPi. The catalytic behavior apparently follows simple Michaelis-Menten kinetics with a Km of about 7 microM for PPi and a specific activity of about 1200 U/mg at 56 degrees C. Surprisingly, maximum activity could be observed at 85 degrees C which is more than 20 degrees C above the temperature for optimal growth. Several cytoplasmic extracts of eubacteria and archaebacteria have been probed with a polyclonal antiserum raised against the purified archaebacterial protein. The only noticeable cross-reactivity could be detected with an extract from the methanogen Methanosarcina barkeri although this probably does not reflect the inferred phylogenetic relationship between methanogens and Thermoplasma acidophilum.
Time synchronization over the Internet using "autolock" (2002)

This paper describes the operation of an algorithm for synchronizing the time of computers using messages transmitted over packet-switched networks such as the Internet. The algorithm configures itself to realize any specified performance level at minimum cost (measured in computer cycles or network bandwidth). If the highest-possible accuracy is requested, the performance will be limited by the larger of the instability of the local clock oscillator or the noise in the measurement process between the client and the server. Lower accuracy can be realized at substantially lower cost. The algorithm uses a frequency-locked loop with unequal spacing between the calibration messages. It makes better use of scarce network bandwidth than previous methods. In addition, the algorithm is an improvement over the pure-FLL "Interlock" algorithm because it is self-configuring. In addition to supporting an explicit trade-off between cost and accuracy, the algorithm provides better performance than previous methods because it is better able to adapt itself to fluctuations in the asymmetry of the network delay. This robustness can be realized without the preliminary tuning that was necessary to realize optimum performance using previous methods.
The Rock Physics Handbook: Tools for Seismic Analysis of Porous Media (2011)

The Rock Physics Handbook addresses the relationships between geophysical observations and the underlying physical properties of rocks. It distills a vast quantity of background theory and laboratory results into a series of concise chapters that provide practical solutions to problems in geophysical data interpretation. This expanded second edition presents major new chapters on statistical rock physics and velocity-porosity-clay models for clastic sediments. Other new and expanded topics include anisotropic seismic signatures, borehole waves, models for fractured media, poroelastic models, and attenuation models. This new edition also provides an enhanced set of appendices with key empirical results, data tables, and an atlas of reservoir rock properties - extended to include carbonates, clays, gas hydrates, and heavy oils. Supported by a website hosting MATLAB routines for implementing the various rock physics formulas, this book is a vital resource for advanced students and university faculty, as well as petroleum industry geophysicists and engineers
Delivery of human mesenchymal adipose-derived stem cells restores multiple urological dysfunctions in a rat model mimicking radical prostatectomy damages through tissue-specific paracrine mechanisms (2015)

Urinary incontinence (UI) and erectile dysfunction (ED) are the most common functional urological disorders and the main sequels of radical prostatectomy (RP) for prostate cancer. Mesenchymal stem cell (MSC) therapy holds promise for repairing tissue damage due to RP. Because animal studies accurately replicating post-RP clinical UI and ED are lacking, little is known about the mechanisms underlying the urological benefits of MSC in this setting. To determine whether and by which mechanisms MSC can repair damages to both striated urethral sphincter (SUS) and penis in the same animal, we delivered human multipotent adipose stem cells, used as MSC model, in an immunocompetent rat model replicating post-RP UI and ED. In this model, we demonstrated by using noninvasive methods in the same animal from day 7 to day 90 post-RP injury that MSC administration into both the SUS and the penis significantly improved urinary continence and erectile function. The regenerative effects of MSC therapy were not due to transdifferentiation and robust engraftment at injection sites. Rather, our results suggest that MSC benefits in both target organs may involve a paracrine process with not only soluble factor release by the MSC but also activation of the recipient's secretome. These two effects of MSC varied across target tissues and damaged-cell types. In conclusion, our work provides new insights into the regenerative properties of MSC and supports the ability of MSC from a single source to repair multiple types of damage, such as those seen after RP, in the same individual.
Pupil shape as viewed along the horizontal visual field (2013)

Changes in pupil size and shape are relevant for peripheral imagery by affecting aberrations and how much light enters and/or exits the eye. The purpose of this study is to model the pattern of pupil shape across the complete horizontal visual field and to show how the pattern is influenced by refractive error. Right eyes of 30 participants were dilated with 1% cyclopentolate, and images were captured using a modified COAS-HD aberrometer alignment camera along the horizontal visual field to ±90°. A two-lens relay system enabled fixation at targets mounted on the wall 3 m from the eye. Participants placed their heads on a rotatable chin rest, and eye rotations were kept to less than 30°. Best-fit elliptical dimensions of pupils were determined. Ratios of minimum to maximum axis diameters were plotted against visual field angle. Participants' data were well fitted by cosine functions with maxima at (-)1° to (-)9° in the temporal visual field and widths 9% to 15% greater than predicted by the cosine of the field angle . Mean functions were 0.99 cos([ + 5.3]/1.121), R(2) 0.99 for the whole group and 0.99 cos([ + 6.2]/1.126), R(2) 0.99 for the 13 emmetropes. The function peak became less temporal and the width became smaller with increase in myopia. Off-axis pupil shape changes are well described by a cosine function that is both decentered by a few degrees and flatter by about 12% than the cosine of the viewing angle, with minor influences of refraction.
Tissue Sensing Adaptive Radar for Breast Cancer Detection—Experimental Investigation of Simple Tumor Models (2005)

Microwave breast cancer detection is based on differences in electrical properties between healthy and malignant tissues. Tissue sensing adaptive radar (TSAR) has been proposed as a method of microwave breast imaging for early tumor detection. TSAR senses all tissues in the volume of interest and adapts accordingly. Simulation results have shown the feasibility of this system for detecting tumors of 4 mm in diameter. In this paper, the second-generation experimental system for TSAR is presented. Materials with electrical properties similar to those in the breast are used for the breast model. A resistively loaded Wu–King monopole antenna is fabricated, and reflections from the breast model over the frequency range of 1–10 GHz are recorded. The reflected signals are processed with the TSAR algorithm, which includes improved skin subtraction and TSAR focusing algorithms. Various tumor models are examined; specifically, a 1-cm tumor is detected with a signal-to-clutter ratio of 10.41 dB. Tumor detection with the experimental system is evaluated and compared to simulation results.
Are Fibrin-Related Markers Useful for the Diagnosis of Thrombosis? (2008)

Fibrin-related markers (FRMs), such as fibrin and fibrinogen degradation products (FDPs), D-dimer, and soluble fibrin (SF), are considered to be useful for the diagnosis of thrombosis. However, the evidence for the making of a diagnosis of thrombosis based on FRMs is, as yet, not fully established. Levels of FRMs are significantly elevated in patients with thrombosis, such as deep vein thrombosis, disseminated intravascular coagulation, and so on. In Japan, the D-dimer assay test result might be 2-fold higher than results for those assays commonly used in Europe and North America. The levels of SF are significantly elevated in patients before the onset of thrombosis, thus suggesting that the SF assay is useful not only for the diagnosis of thrombosis but also for diagnosing a prethrombotic state. Overall, elevated levels of FRMs indicate a high risk for thrombosis, and they are thus considered to be useful for the diagnosis of thrombosis.
Interactions of water with OH groups in poly(ethylene‐<i>co</i>‐vinyl alcohol) (2006)

Abstract We studied interactions of water with OH groups in poly(ethylene‐ co ‐vinyl alcohol) (EVOH) of the ethylene (Et) compositions of 33, 49, 75, and 89% (mol) using near‐infrared spectroscopy. OH groups are mostly self‐associated and only partly isolated in EVOH matrix, and the fraction of the isolated OH increases with the increase in Et composition. Interactions of water in EVOH were analyzed on the basis of the difference spectrum between the spectrum in a hydrated state and that in the dehydrated state of a sample. The water contained in an EVOH sample with a higher Et content shows a more distinct sharp combination band at about 5285 cm − 1 in addition to the main broad band at about 5150 cm − 1 . The former was assigned to the water that is one‐to‐one hydrogen‐bonded to an isolated OH in the manner as OH::OH 2 from the effect of the hydration on the near‐infrared and infrared bands of the isolated OH. The latter was assigned to the water that is multiply hydrogen‐bonded to surrounding associated OH groups and that does not cluster. © 2006 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 44: 2425–2437, 2006
Polyadenylation precedes splicing in vitro. (1991)

Vertebrate premessenger RNAs are usually spliced and polyadenylated. In vivo analysis of the relative kinetics of the two reactions is difficult. We have used in vitro processing systems to investigate the order of splicing and polyadenylation of chimeric precursor RNAs containing a single intron and a poly(A) site. Polyadenylated, but not spliced, intermediate RNA appeared first and reached a low steady-state level early during incubation, properties consistent with its being a reaction intermediate in the production of doubly-processed spliced and polyadenylated product RNA. The kinetics of polyadenylation suggested that polyadenylated RNA was the only intermediate in the production of doubly-processed RNA. Spliced, but not polyadenylated, RNA also appeared. This species, however, continued to accumulate during reaction, and could not be chased into product spliced and polyadenylated RNA. These data support a preferred order of reaction for 3' terminal introns and exons in which polyadenylation precedes splicing.
Structure‐Activity Relationships of Pyrazine‐Based CK2 Inhibitors: Synthesis and Evaluation of 2,6‐Disubstituted Pyrazines and 4,6‐Disubstituted Pyrimidines (2008)

Structurally related to the known CK2 inhibitors, 2,6-disubstituted pyrazine and 4,6-disubstituted pyrimidine derivatives were synthesized and their inhibitory activities toward CK2alpha and CK2alpha' were evaluated. Structure-activity relationship study has revealed that several pyrazine derivatives bearing a (pyrrol-3-yl)acetic acid and a monosubstituted aniline possess potent inhibitory activities.
The Fur regulon in anaerobically grown Salmonella enterica sv. Typhimurium: identification of new Fur targets (2011)

This study determined that Fur is a positive factor in ftnB regulation, while serving to repress the expression of hmpA. Furthermore, Fur is required for the proper expression and activation of the antioxidant enzymes, FeSOD and MnSOD. Finally, this work identified twenty-six new targets of Fur regulation, and demonstrates that H-NS repressed genes are down-regulated in Δfur.
A pilot, double-blind, controlled 1-year trial of prednisolone treatment in primary biliary cirrhosis: Hepatic improvement but greater bone loss (1989)

A randomized, double-blind, 1-year pilot study of prednisolone treatment for primary biliary cirrhosis was undertaken. Nineteen patients received 30 mg prednisolone per day initially, with a maintenance dose of 10 mg per day. Seventeen patients received placebo. The groups were matched for age, menopausal status, hepatic histological stage and bilirubin. Treatment was well tolerated without dropouts. Two patients receiving prednisolone developed diabetes, one a duodenal ulcer and one depression. One patient receiving placebo died for liver failure after 3 months. Cholestatic symptoms (itch and fatigue) improved on prednisolone. There was significant (prednisolone vs. placebo) improvement in transaminase (p = 0.0214), alkaline phosphatase (p = 0.0032), procollagen III peptide (p = 0.0103), immunoglobulin G (p = 0.0012) and liver histology (p = 0.016); these changes were greatest among noncirrhotic patients. No patient developed skeletal symptoms. Fifty-seven per cent had abnormal triolein breath tests prior to treatment, and 65% had abnormally low calcium absorption tests. Calcium absorption increased significantly in the treated group vs. placebo at 2 weeks (p less than 0.02), but not at 1 year. Femoral photon absorptiometry fell in the prednisolone group after 1 year (-3.5% vs. placebo +0.5%, p less than 0.05), as did trabecular bone volume (-6% vs. -2.8%, p less than 0.005) and resorption surface (-11% vs. +2%, p less than 0.02) on serial bone biopsy. Prednisolone seems to exert a favorable hepatic effect in primary biliary cirrhosis but at the expense of increased bone loss to approximately twice the expected rate. Prednisolone treatment merits further assessment in primary biliary cirrhosis over a longer period, with attention to selection of patients most likely to benefit and continuing observation of bone mass to better establish the "cost/benefit" ratio.
Crystalline selenite substituted carbonated hydroxyapatite nanorods: Synthesis, characterization, evaluation of bioactivity and cytotoxicity (2016)

Synthesis of pure and selenite substituted hydroxyapatites (HAP and Se‐HAP with 0.02–0.10 Se) by sol–gel method and evaluation of their morphological features, nature of functional groups, phase purity, in vitro bioactivity and cytotoxicity are addressed. Spectral studies confirm the incorporation of selenite into the HAP lattice, accompanied by an increase in CO 3 2− content to maintain the charge imbalance following replacement of P 5+ with Se 4+ , thus making the formation of selenite substituted carbonated HAP. Selenite substitution in the HAP lattice has led to a higher crystallinity and increased the crystallite size. The morphology of HAP is changed from sphere to rod‐like structure upon substitution by selenite and the size of the rod is increased with an increase in the selenite content. Among the Se‐HAPs, a better in vitro bioactivity and cell viability are observed for 0.02 Se‐HAP and 0.04 Se‐HAP while the trend is reversed when the extent of selenite substitution becomes higher.
A new optically read ferroelectric memory (1967)

The optically read ferroelectric memory described utilizes nondestructive optical reading made possible by the unique switching and optical properties of single-crystal ferroelectric bismuth titanate (Bi <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> Ti <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">12</inf> ). The use of known photoconductor access techniques for the writing operation in conjunction with Bi <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</inf> Ti <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> O <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">12</inf> crystals provides a completely optically accessed ferroelectric memory.
High-content adhesion assay to address limited cell samples (2013)

Cell adhesion is a broad topic in cell biology that involves physical interactions between cells and other cells or the surrounding extracellular matrix, and is implicated in major research areas including cancer, development, tissue engineering, and regenerative medicine. While current methods have contributed significantly to our understanding of cell adhesion, these methods are unsuitable for tackling many biological questions requiring intermediate numbers of cells (10(2)-10(5)), including small animal biopsies, clinical samples, and rare cell isolates. To overcome this fundamental limitation, we developed a new assay to quantify the adhesion of ~10(2)-10(3) cells at a time on engineered substrates, and examined the adhesion strength and population heterogeneity via distribution-based modeling. We validated the platform by testing adhesion strength of cancer cells from three different cancer types (breast, prostate, and multiple myeloma) on both IL-1β activated and non-activated endothelial monolayers, and observed significantly increased adhesion for each cancer cell type upon endothelial activation, while identifying and quantifying distinct subpopulations of cell-substrate interactions. We then applied the assay to characterize adhesion of primary bone marrow stromal cells to different cardiac fibroblast-derived matrix substrates to demonstrate the ability to study limited cell populations in the context of cardiac cell-based therapies. Overall, these results demonstrate the sensitivity and robustness of the assay as well as its ability to enable extraction of high content, functional data from limited and potentially rare primary samples. We anticipate this method will enable a new class of biological studies with potential impact in basic and translational research.