gebhart/patrepeval huggingface dataset:
Optimizing the Balance Between Host and Environmental Survival Skills: Lessons Learned from <i>Listeria monocytogenes</i> (2012)

Environmental pathogens - organisms that survive in the outside environment but maintain the capacity to cause disease in mammals - navigate the challenges of life in habitats that range from water and soil to the cytosol of host cells. The bacterium Listeria monocytogenes has served for decades as a model organism for studies of host-pathogen interactions and for fundamental paradigms of cell biology. This ubiquitous saprophyte has recently become a model for understanding how an environmental bacterium switches to life within human cells. This review describes how L. monocytogenes balances life in disparate environments with the help of a critical virulence regulator known as PrfA. Understanding L. monocytogenes survival strategies is important for gaining insight into how environmental microbes become pathogens.
Broad selective efficacy of recombinant methioninase and polyethylene glycol-modified recombinant methioninase on cancer cells In Vitro. (2010)

Elevated dependence on methionine of cancer cells is a cancer-specific metabolic defect. Recombinant L-methionine alpha,gamma-lyase (rMETase), an L-methionine depleting enzyme cloned from Pseudomonas putida, was shown to have efficacy on a broad series of cancer cell lines. Twenty-one different human tumor cell lines (4 lung, 4 colon, 4 kidney, 4 melanoma, 3 CNS, and 2 prostate) from the NCI Human Tumor Cell Line Screen and 7 human normal cell strains were treated with rMETase in vitro. We showed that rMETase had a mean IC(50) (units rMETase/ml) for the following cancer cell types: renal, 0.07; colon, 0.04; lung, 0.12; prostate, 0.01; melanoma, 0.19; and CNS, 0.195, which was approximately one order of magnitude lower than that for normal cell strains: skin fibroblasts, 4; aortic smooth muscle cells, 0.88; aortic endothelial cells, 0.8; keratinocytes, 0.75; and bronchial epithelial cells, 0.75. rMETase was also conjugated with polyethylene glycol (PEG). PEG-rMETase also had high cancer cell-killing activity. In vitro studies, animal studies and clinical trials have now shown that methionine restriction is an effective anticancer strategy. Cells from many different types of cancer are methionine dependent. The most effective strategy to deplete methionine is with the use of rMETase. PEG-rMETase offers additional advantages of increasing the circulating half-life and reducing the immunogenicity of rMETase which is a bacterial protein. The results of the current study demonstrate the broad clinical potential for rMETase and PEG-rMETase for cancer treatment.
Synthesis of 2,6-dialkylphenyl 4-nitrophenyl ethers from highly hindered phenols (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of 2,6-dialkylphenyl 4-nitrophenyl ethers from highly hindered phenolsJeremy Wright and Eugene C. JorgensenCite this: J. Org. Chem. 1968, 33, 3, 1245–1246Publication Date (Print):March 1, 1968Publication History Published online1 May 2002Published inissue 1 March 1968https://pubs.acs.org/doi/10.1021/jo01267a069https://doi.org/10.1021/jo01267a069research-articleACS PublicationsRequest reuse permissionsArticle Views168Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cimetidine Suppression of Nocturnal Gastric Secretion in Active Duodenal Ulcer (1976)

Nocturnal parn and gastric hypersecretion are common in duodenal ulcer. Therefore, we investigated the antisecretory effects of a new H2-receptor antagonist, cimetidine, in 200-, 300- or 400-mg doses, taken orally at bedtime. The 200-mg dose did not cause a statistically significant change in nocturnal (midnight to 7 a.m.) acid output and had only a borderline effect on pH. However, the 300-mg and 400-mg doses significantly (P < 0.001) lowered acid output and increased (P < 0.01) intragastric pH. All doses caused substantial decreases in secretory volume output. After a 400-mg dose, half the patients remained anacidic for eight hours. Dose-related increases of drug blood levels were observed and correlated with the degree and duration of inhibition of acid output. Serum gastrin levels were unaffected. Cimetidine appears to be a potent inhibitor of nocturnal gastric secretion. (N Engl J Med 294:801–804, 1976)
<i>CalpA</i>, a <i>Drosophila</i> Calpain Homolog Specifically Expressed in a Small Set of Nerve, Midgut, and Blood Cells (1995)

Calpains are calcium-dependent proteases believed to participate in calcium-regulated signal pathways in cells. Ubiquitous calpains as well as tissue-specific calpains have been found in vertebrates. We isolated cDNA clones for a highly tissue-specific calpain gene from Drosophila melanogaster, CalpA, at 56C-D on the second chromosome. The expression of the CalpA gene product was monitored by using a specific antiserum directed against the product expressed by one cDNA clone. The encoded protein is found in a few neurons in the central nervous system, in scattered endocrine cells in the midgut, and in blood cells. In the blood cell line mbn-2, calpain is associated with a granular component in the cytoplasm. The expression of this protein is more restricted than that of the corresponding transcripts, which are widely distributed in the central nervous system, digestive tract, and other tissues. The sequence of CalpA is closely related to that of vertebrate calpains, but an additional segment is inserted in the calmodulin-like carboxy-terminal domain. This insert contains a hydrophobic region that may be involved in membrane attachment of the enzyme. Differential splicing also gives rise to a minor transcript that lacks the calmodulin-like domain.
Responses of Thin Film Photovoltaic Cell to Irradiation under Double Laser Beams of Different Wavelength (2013)

During the process of Laser beam long-range energy transmission based on the photovoltaic principle, the temperature elevation of the photovoltaic cell under Laser irradiation would greatly affect its photoelectric conversion efficiency. In this work, the heat generation mechanism of thin-film photovoltaic cell under Laser irradiation was analyzed based on the energy conversion relationship between light, electricity and heat. The output voltage characteristic of a thin film GaInP/GaAs/Ge triple-junction photovoltaic cell under irradiation of dual-wavelength Laser beam was investigated, and the results show the dependence of the conversion efficiency on the temperature and the valve effect, which was caused by the long wavelength Laser and would sharply reduce the photovoltaic conversion efficiency of short wavelength Laser. The temperature field of the multi-layer structure subjected to Laser irradiation was analyzed by finite element method, and the results indicate that the temperature elevation pattern depends on the spatial distribution of the light absorption and can be adjusted via optimizing the photovoltaic cell structure.
Enzyme-Linked Immunoassay for Detection of Listeria monocytogenes in Dairy Products, Seafoods, and Meats: Collaborative Study (1994)

A collaborative study was conducted to evaluate Listeria-Tek, an enzyme-linked immunosorbent assay (ELISA) for detection of Listeria monocytogenes and other Listeria spp. in foods. The present ELISA method was compared to the U.S. Food and Drug Administration culture method for detection of L. monocytogenes in dairy products and seafoods and to the U.S. Department of Agriculture Food Safety and Inspection Service method for detection of L. monocytogenes in meats. Replicate samples of 6 food types (frankfurters, roast beef, Brie cheese, 2% milk, raw shrimp, and crab meat) inoculated with L. monocytogenes and uninoculated control samples were analyzed by the collaborators. L. monocytogenes was identified in 593 samples by the ELISA method and in 574 samples using culture procedures. Identical results were obtained for 506 positive samples and 419 negative samples using the ELISA and culture methods for an overall agreement rate of 85.6%. The enzyme-linked immunoassay for detection of L. monocytogenes in dairy, seafood, and meat products has been adopted first action by AOAC INTERNATIONAL.
Chronic Venous Insufficiency (2005)

Chronic venous insufficiency, characterized by the retrograde flow of blood in the lower extremity, is a common, debilitating disorder that is increasing in prevalence. An estimated 25 million people in the United States have varicose veins, 2 to 6 million have more advanced forms of chronic venous insufficiency (swelling, skin changes), and nearly 500,000 have painful venous ulcers. Although varicose veins are more likely to develop in women, men more commonly have venous ulcers. Regardless of gender, the disorder is associated with a significant reduction in quality of life. Although initiating events, such as deep vein thrombosis and muscle pump failure, may lead to chronic venous insufficiency, vein valve dysfunction appears to be the common final pathway in the development of this problem. Recent studies have determined that the basal state of veins valves is coaptation, with the prevention of retrograde flow. Failure of this mechanism results in increases in retrograde flow and distal venous pressure, which can begin to alter the structure and function of the downstream vein wall. The diagnosis is established by history, physical examination, and functional venous testing with duplex imaging or phlebography. Classification systems have been developed that stratify according to the severity of the disorder and help guide therapeutic endeavors. Treatment is directed toward controlling the retrograde flow of blood, venous pooling, and the complications associated with these pathophysiologic processes. When appropriate therapy is instituted, response is generally excellent.
Temperature determination in optoelectronic waveguide modulators (2000)

Optoelectronic devices are particularly sensitive to temperature changes induced by the absorption of light and the passage of current. In order to study the thermal issues arising in a InGaAsP-based Mach-Zehnder (MZ) optical modulator, a nonlinear finite-element thermal model of the device was constructed. The model considers the variation with temperature of both the thermal conductivity of the semiconductors composing the device and the optical absorption. To that effect, the optical absorption was measured inside the waveguide as a function of temperature, An experimental method using liquid crystals to measure the surface temperature was also developed. Both were used to evaluate the temperature inside a variable optical attenuator present on the modulator. Good agreement with the model and the experiment is found over a wide range of operating conditions. These tools are expected to play a key role in understanding thermal issues in future photonic devices, in view of the desire to integrate multiple devices on a common substrate and the continuous increase of the optical powers in fiber systems.
Suppressive expression of <scp>CD</scp>274 increases tumorigenesis and cancer stem cell phenotypes in cholangiocarcinoma (2014)

Cholangiocarcinoma is an aggressive malignant tumor originating from intrahepatic or extrahepatic bile ducts. Its malignant phenotypes may be assumed by cancer stem cells (CSC). Here, we demonstrate that CD274 (PD-L1), known as an immunomodulatory ligand, has suppressive effects on CSC-related phenotypes of cholangiocarcinoma. Using two human cholangiocarcinoma cell lines, RBE and HuCCT1, we attempted to isolate the CD274(low) and CD274(high) cells from each cell line, and xenografted them into immunodeficient NOD⁄scid⁄γcnull (NOG) mice. We found that the CD274(low) cells isolated from both RBE and HuCCT1 are highly tumorigenic in NOG mice compared with CD274(high) cells. Furthermore, the CD274(low) cells possess several CSC-related characteristics, such as high aldehyde dehydrogenase (ALDH) activity, reduced reactive oxygen species production and a dormant state in the cell cycle. Furthermore, depletion of CD274 expression by shRNA in RBE cells enhances their tumorigenicity and increases ALDH activity. These findings are compatible with our observation that clinical cholangiocarcinoma specimens are classified into low and high groups for CD274 expression, and the CD274 low group shows poorer prognosis when compared with the CD274 high group. These results strongly suggest that CD274 has a novel function in the negative regulation of CSC-related phenotypes in human cholangiocarcinoma, which is distinct from its immunomodulatory actions.
Plant Poisoning Of Livestock (1945)

One of the most difficult problems of the practicing veterinarian is the diagnosis of toxic symptoms due to poisonous plants. Some plants produce in animals symptoms quite similar to those indicative of an infectious or non-infectious disease. Consequently, the diagnosis of plant poisoning apart from pathological conditions from other causes may be difficult.
Epstein-Barr Virus Nuclear Antigen-1-Dependent and -Independent <i>oriP</i>-Binding Cellular Proteins (2001)

<i>Objective:</i> Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) and the replication origin, <i>oriP</i>, are essential for the replication and maintenance of latent EBV DNA in cells, but no enzymatic activity has been associated with EBNA-1 protein alone. In this study, we have searched for host cellular proteins that interact with EBNA-1 protein in various B cell lines latently infected with EBV, including a recently EBV growth-transformed cell line. <i>Methods:</i> By using gel shift analysis, we investigated the interactions of an oligonucleotide containing a single EBNA-1 recognition site, derived from the family of repeats (FR) element of <i>oriP</i>, with protein from cell extracts. <i>Results:</i> The FR oligonucleotide bound a (72-kD) cellular protein in the absence of EBNA-1 and without induction of the previously reported ‘anti-EBNA-1 proteins’. The FR oligonucleotide formed complexes with additional proteins from EBNA-1-synthesizing cell lines; these complexes were abolished or supershifted by anti-EBNA-1 monoclonal antibodies. SDS-PAGE analyses of <sup>35</sup>S-Met-labeled proteins that bound to a biotin- conjugated FR oligonucleotide, fractionated by a glycerol gradient centrifugation and affinity-purified with streptavidin, showed three major bands, a 72-kD protein, the FR binding of which seemed to be independent of EBNA-1, a 64-kD protein in both EBNA-1-transfected and latently EBV-infected cell lines, and a 45-kD protein in EBV-infected cell lines, which was most prominent in a recently EBV growth-transformed cell line. <i>Conclusions:</i> The FR element forms complexes with cellular proteins in the absence and presence of EBNA-1. These 72-, 64- and 45-kD cellular proteins might be involved in the function of the <i>oriP</i> and EBNA-1 system.
Effect of pulsed electromagnetic field treatment on programmed resolution of inflammation pathway markers in human cells in culture (2015)

Inflammation is a complex process involving distinct but overlapping biochemical and molecular events that are highly regulated. Pulsed electromagnetic field (PEMF) therapy is increasingly used to treat pain and edema associated with inflammation following surgery involving soft tissue. However, the molecular and cellular effects of PEMF therapy on pathways involved in the resolution of inflammation are poorly understood. Using cell culture lines relevant to trauma-induced inflammation of the skin (human dermal fibroblasts, human epidermal keratinocytes, and human mononuclear cells), we investigated the effect of PEMF on gene expression involved in the acute and resolution phases of inflammation. We found that PEMF treatment was followed by changes in the relative amount of messenger (m)RNAs encoding enzymes involved in heme catabolism and removal of reactive oxygen species, including an increase in heme oxygenase 1 and superoxide dismutase 3 mRNAs, in all cell types examined 2 hours after PEMF treatment. A relative increase in mRNAs encoding enzymes involved in lipid mediator biosynthesis was also observed, including an increase in arachidonate 12- and 15-lipoxygenase mRNAs in dermal fibroblasts and epidermal keratinocytes, respectively. The relative amount of both of these lipoxygenase mRNAs was elevated in mononuclear cells following PEMF treatment relative to nontreated cells. PEMF treatment was also followed by changes in the mRNA levels of several cytokines. A decrease in the relative amount of interleukin 1 beta mRNA was observed in mononuclear cells, similar to that previously reported for epidermal keratinocytes and dermal fibroblasts. Based on our results, we propose a model in which PEMF therapy may promote chronic inflammation resolution by mediating gene expression changes important for inhibiting and resolving inflammation.
Microbiology of the Red Sea (and other) deep‐sea anoxic brine lakes (2011)

The Red Sea harbours approximately 25 deep-sea anoxic brine pools. They constitute extremely unique and complex habitats with the conjugation of several extreme physicochemical parameters rendering them some of the most inhospitable environments on Earth. After 50 years of research mostly driven by chemists, geophysicists and geologists, the microbiology of the brines has been receiving increased interest in the last decade. Recent molecular and cultivation-based studies have provided us with a first glimpse on the enormous biodiversity of the local microbial communities, the identification of several new taxonomic groups, and the isolation of novel extremophiles that thrive in these environments. This review presents a general overview of these unusual biotopes and compares them with other similar environments in the Mediterranean Sea and the Gulf of Mexico, with a focus on their microbial ecology.
Population pharmacokinetic and pharmacodynamic analysis of tremelimumab in patients with metastatic melanoma (2014)

Tremelimumab, a fully human monoclonal antibody specific for human cytotoxic T-lymphocyte-associated antigen 4, has been studied in clinical trials. We have reported the results of population pharmacokinetics for tremelimumab in 654 metastatic melanoma patients. Population estimates (inter-individual variability [IIV]) for pharmacokinetic parameters in a final model were clearance (CL), 0.26 L/day (31.8%) and central volume of distribution, 3.97 L (20.4%). CL was faster in males, patients with higher values of creatinine clearance and endogenous immunoglobulin, and patients with relatively poor baseline prognostic factors. No dose adjustment was needed based on the magnitude of the change of CL (<30%). The association of CL and overall survival (OS) was investigated. In a Phase 3 trial evaluating tremelimumab as first-line-treatment, median OS for the 147 patients in the fast-CL group (≥ median CL value) was 9.6 months versus 15.8 months for the 146 patients in the slow-CL group (<median CL value). Multiple Cox proportional hazard regression models were constructed to evaluate the association between CL and OS adjusting for the effects of baseline prognostic covariates between two CL groups. The analysis showed statistically significant association between CL and OS (P < .05). Results suggest that higher or more frequent dosing should be considered in future trials.
A PEEC with a new capacitance model for circuit simulation of interconnects and packaging structures (2000)

In this paper, a modified partial-element equivalent-circuit (PEEC) model, i.e., (L/sub p/, A/spl I.oarr/, R, /spl epsiv/f)PEEC, is introduced. In such a model, no equivalent circuit, but a set of state equations for the variables representing the function of circuit, are given to model a three-dimensional structure. Unlike the original (L/sub p/, P, R, /spl epsiv/f) PEEC model, the definition of vector potential A/spl I.oarr/ with integral form and the Lorentz gauge are used in expanding the basic integral equation instead of the definition of the scalar potential /spl phi/ with integral form. This can directly lead to the state equations, and the capacitance extraction can be replaced by the calculation of the divergence of A/spl I.oarr/, which is analytical. For analysis of most interconnect and packaging problems, generally containing complex dielectric structures, the new model can save a large part of computing time. The validity of the new model is verified by the analysis in time and frequency domain with several examples of typical interconnect and packaging structures, and the results with this new method agree well with those of other papers.
Radiolabelled granulocytes in inflammatory bowel disease (1988)

Radiolabelled granulocytes in chronic inflammatory bowel diseases (CIBD) are able to diagnose the disease extent and assess the disease activity. It may be performed with 111In oxine- and 99Tcm hexamethylpropyleneamineoxime (HMPAO)-labelled cells. The granulocyte scan localizes inflamed bowel segments with an accuracy comparable with radiology and endoscopy including biopsy of the bowel (r = 0.95; P less than 0.001). The specificity of the scan for diseased segments is near 100%, the sensitivity 92%. A three-phase white blood cell scan (imaging: 0.5, 4, 20 h post injection) allows differentiation of diseased bowel segments from abscesses and fistulas. False positive results are possible in necrotic carcinomas. The 99Tcm HMPAO scan shows rapid renal and delayed biliary and intestinal excretion of tracer. In this way diagnostic problems arise in the small pelvis. Because of the intestinal and biliary excretion, early images should be obtained (0.5-2 h post injection). Later scans with 99Tcm HMPAO are of minor importance. The disease activity can very specifically be assessed by the determination of the percentage faecal 111In excretion (96 h faecal collection). Active and non-active diseases can be clearly differentiated. We found no correlation with the subjectively influenced Crohn's disease activity index (CDAI) (r = 0.25; P greater than 0.05), but good correlations with the Dutch Index (van Hees: r = 0.67), ESR (r = 0.69), serum albumin (r = -0.54) and orosomucoid (r = 0.65). The percentage faecal excretion correlates well with the histologically estimated leucocytic bowel infiltration. Because of the intestinal 99Tcm HMPAO excretion the determination of faecal excretion is pointless in 99Tcm studies.(ABSTRACT TRUNCATED AT 250 WORDS)