gebhart/patrepeval huggingface dataset:
Identification of a guanine binding domain peptide of the GTP binding site of glutamate dehydrogenase: Isolation with metal-chelate affinity chromatography (1993)

Photoaffinity labeling with [alpha-32P]8N3GTP and [gamma-32P]8N3GTP was used to identify the guanine binding domain of the GTP regulatory site within glutamate dehydrogenase (GDH). Without photolysis, 8N3GTP mimicked the regulatory properties of GTP on GDH activity with 8N3GTP exhibiting a Ki of 5 microM while the Ki for GTP was about 0.6 microM. Under optimal photolabeling conditions saturation of photoinsertion with 1 microgram of GDH revealed an apparent Kd of 9 +/- 4 microM for [gamma-32P]8N3GTP. Photolabeling with this analog could be competitively inhibited with GTP with an apparent Kd of 12 +/- 2 microM. Other nucleotides such as ATP and NAD(P)H could not reduce the amount of photoinsertion as effectively as GTP. ADP could decrease photoinsertion, but only at much higher concentrations. NAD(P)+, GDP, AMP, and GMP had little effect on photoinsertion. Divalent cations Mg2+ and Ca2+ also reduced photoinsertion significantly while the monovalent K+ and Na+ ions had no effect. Aluminum(III)-chelate or iron(III)-chelate affinity chromatography and reversed-phase HPLC were used to purify photolabel-containing peptides generated with either trypsin or chymotrypsin. This identified a portion of the guanine binding domain within the GTP regulatory site as the region containing the sequence Ile439 to Tyr454. Photolabeling of this peptide was prevented 91% by the presence of 300 microM GTP during photolysis. Lys445 was not identified in sequence analyses of the photolabeled peptides. Also, trypsin was unable to cleave the photolabeled peptide at this site. These results suggest that Lys445 may be the residue modified by [alpha-32P]8N3GTP.
Remote Sensing Technologies for Detecting, Visualizing and Quantifying Gas Leaks (2018)

Abstract Remote sensing technologies can be applied for a wide range of gas leak flowrates and in three main cases: (1) major leaks in crisis management; (2) medium size leaks in safety monitoring; (3) small leaks in environmental monitoring. A gas test campaign, conducted by Total, the ONERA – the French Aerospace Lab – and ADCIS in September 2015 using three hyperspectral infrared cameras from Telops, confirmed our capacity to visualize in 3D and quantify in real time plumes of methane in the range of 1 g/s to 50g/s. The R&D project on gas remote quantification continued with a second gas test campaign in 2017. The second gas test campaign was organized on Total's Lacq Pilot Platform in France and involved several gas spectral imaging systems: (1) mobile hyperspectral cameras in the Long-Wavelength InfraRed (LWIR) band (7.7-12μm); (2) a multispectral camera in the LWIR band (7-9μm); (3) a multigas lidar (LIght Detection And Ranging) system coupled with a wind lidar system; (4) five other international teams (US, Spain, Norway and France) were also invited to assess the capacity of their remote-sensing systems to quantify methane and carbon dioxide releases. The two-week test demonstrated that methane leak emissions ranging from 0.7 g/s to 140 g/s could be visualized and quantified in real time using a mobile Telops Hyper-Cam. This campaign also served to validate the performance of several remote sensing technologies. Total's Lacq Pilot Platform is a test area for qualifying cost-effective systems designed to complement the gas detection system of a plant and provide valuable information should a gas leak incident occur. New methodologies for the early detection of anomalies using remote observation systems including drones, robots and artificial intelligence data processing systems are currently being investigated there.
The Springs of Virginia: Life, Love, and Death, 1775-1900. By Perceval Reniers. (Chapel Hill: University of North Carolina Press, 1941. xl + 301 pp. Appendix, bibliography, and illustrations. $4.00.) (1942)

The Springs of Virginia: Life, Love, and Death, 1775–1900. By Perceval Reniers. (Chapel Hill: University of North Carolina Press, 1941. xl + 301 pp. Appendix, bibliography, and illustrations. $4.00.) Get access Kathleen Bruce Kathleen Bruce Historical Records Survey of Virginia Search for other works by this author on: Oxford Academic Google Scholar Journal of American History, Volume 29, Issue 2, September 1942, Pages 287–288, https://doi.org/10.1093/jahist/29.2.287 Published: 01 September 1942
Explaining control strategies in problem solving (1989)

Explaining how knowledge-based systems reason involves presentation user modeling, dialogue structure, and the way systems understand their own problem-solving knowledge and strategies. The authors concentrate on the last of these, noting that such understanding provides any explanations's content. The authors also note that most current approaches to knowledge-based system construction require expressing knowledge and control at such low levels that it's hard to give high-level explanations. Providing an explanation example from a prototypical system (MYCIN) built using generic-task methods, they propose generic-task methodology as one way to build knowledge-based systems that contain basic explanation constructs at appropriate abstraction levels. The central concept of generic tasks is what input-output behavior (i.e. that task function), knowledge needed to perform the task, and inferences appropriate for the task are all specified together.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Prevalence and genetic diversity of human caliciviruses (HuCVs) in Mexican children (2000)

Human caliciviruses (HuCVs) contain two genera: “Norwalk-like viruses” (NLVs) and “Sapporo-like viruses” (SLVs). The importance of the two genera as a cause of acute gastroenteritis of infants and children remains unknown. Beginning in 1989, a birth cohort of children in Mexico was enrolled and monitored for acute gastroenteritis. A subset of 115 diarrhea stool specimens from 76 children and 66 non-diarrhea stool specimens from 64 children was examined for HuCVs by RT-PCR by using a primer pair (p289/290) that detects both NLVs and SLVs. Twenty-two (19%) of the 115 diarrhea stool specimens and 5 (7%) of 66 non-diarrhea stool specimens produced RT-PCR products of expected size (319 bp for NLVs and 331 bp for SLVs). Twenty of the twenty-seven strains were cloned and sequenced. Pairwise sequence analysis showed that 9 (60%) and 6 (40%) of the 15 strains from the diarrhea stools were NLVs and SLVs, respectively. The same proportions of NLVs (60%) and SLVs (40%) were observed in the non-diarrhea stools. Strains in the NLV genus could be further divided into four clusters: Lordsdale, MxV, and HV and one potentially new cluster. Strains in the SLV genus could be divided into three clusters: Sapporo/82, Lon/92, and a potentially new cluster. Strains from the Lordsdale cluster were the most common among these children. The findings of both genera and multiple clusters of HuCVs co-circulating and the identification of new strains of HuCVs in the population justify the need for future studies of HuCVs in infants and children. J. Med. Virol. 62:217–223, 2000. © 2000 Wiley-Liss, Inc.
New formalism for bending losses in coated single-mode optical fibres (1989)

A new method to estimate the losses in bent weakly guiding optical fibres is described, taking into account the finite dimensions of the fibre cross-section. A comparison between theoretical and experimental results is presented to demonstrate the validity of this theory.
Lispro Mix25 insulin as premeal therapy in type 2 diabetic patients. (1999)

Because of its faster initial absorption rate, the new premixed insulin analog Mix25 reduces blood glucose response to a breakfast meal in type 2 diabetic patients compared with premixed 30/70 (regular/NPH) or NPH insulin.
Epitaxial regrowth of thin amorphous GaAs layers (1981)

Channeling and transmission electron microscopy have been used to investigate the parameters that govern the crystal quality following capless funace annealing at low temperature (∼ 400 °C) in ion-implanted GaAs. From the results obtained, we concluded that the crystal quality after annealing depends strongly on the thickness of the amorphous layer generated by ion implantation and the number of residual defects increases linearly with the thickness of the implanted layer. Single-crystal regrowth free of defects detectable by megaelectron volt He+ channeling was achieved for a very thin amorphous layer (≲ 400 Å).
Spektral abstimmbare IR-Sensoren für die industrielle Prozessmesstechnik und die medizinische Gasanalyse (2012)

Zusammenfassung Mikrospektrometer besitzen ein großes Potenzial in der IR-Absorptionsspektroskopie. Im Beitrag werden durchstimmbare IR-Sensoren für das mittlere und langwellige Infrarot vorgestellt, welche auf pyroelektrischen Detektoren und mikromechanischen Fabry-Pérot-Filtern basieren. Ausgewählte Beispielapplikationen aus der medizinischen und industriellen Analytik werden erläutert und ein Ausblick auf eine neue Generation höher auflösender und schnellerer FP-Spektrometer gegeben.
Specific cleavage of simian virus 40 DNA by restriction endonuclease of <i>Hemophilus influenzae</i> (1971)

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.
Suppression of arthritis and protection from bone destruction by treatment with TNP‐470/AGM‐1470 in a transgenic mouse model of rheumatoid arthritis (2000)

Our demonstration that in vivo administration of an angiogenesis inhibitor suppresses arthritis and protects from bone destruction provides new insight into the pathogenesis of the disease and opens new possibilities in the treatment of RA in humans.
Biomedically relevant circuit‐design strategies in mammalian synthetic biology (2013)

The development and progress in synthetic biology has been remarkable. Although still in its infancy, synthetic biology has achieved much during the past decade. Improvements in genetic circuit design have increased the potential for clinical applicability of synthetic biology research. What began as simple transcriptional gene switches has rapidly developed into a variety of complex regulatory circuits based on the transcriptional, translational and post-translational regulation. Instead of compounds with potential pharmacologic side effects, the inducer molecules now used are metabolites of the human body and even members of native cell signaling pathways. In this review, we address recent progress in mammalian synthetic biology circuit design and focus on how novel designs push synthetic biology toward clinical implementation. Groundbreaking research on the implementation of optogenetics and intercellular communications is addressed, as particularly optogenetics provides unprecedented opportunities for clinical application. Along with an increase in synthetic network complexity, multicellular systems are now being used to provide a platform for next-generation circuit design.
A large fraction of neocortical myelin ensheathes axons of local inhibitory neurons (2016)

Myelin is best known for its role in increasing the conduction velocity and metabolic efficiency of long-range excitatory axons. Accordingly, the myelin observed in neocortical gray matter is thought to mostly ensheath excitatory axons connecting to subcortical regions and distant cortical areas. Using independent analyses of light and electron microscopy data from mouse neocortex, we show that a surprisingly large fraction of cortical myelin (half the myelin in layer 2/3 and a quarter in layer 4) ensheathes axons of inhibitory neurons, specifically of parvalbumin-positive basket cells. This myelin differs significantly from that of excitatory axons in distribution and protein composition. Myelin on inhibitory axons is unlikely to meaningfully hasten the arrival of spikes at their pre-synaptic terminals, due to the patchy distribution and short path-lengths observed. Our results thus highlight the need for exploring alternative roles for myelin in neocortical circuits.
New product development: using the salesforce to identify opportunities (1997)

The development process for new products is critically dependent on customer‐generated new product ideas. Although several conduits exist for identifying and communicating these ideas, by far the most productive one is the organization’s salesforce. While the integral role of salespeople as the linkage between buyers and sellers is generally acknowledged by many researchers, little empirical evidence exists which explores this role. This paper explores the role of the salesforce as an information source in the opportunity identification phase of the new product development process. It presents results of an empirical study of 223 sales managers concerning actual use and effectiveness of the salesforce as a source of new product ideas and proposes specific recommendations concerning improved utilization of the salesforce as a source of new product ideas generated from customers.
Transition-aware task scheduling and configuration selection in reconfigurable embedded systems (2013)

This paper presents a novel graph representation that captures the transition overhead due to runtime configuration of underlying hardware in reconfigurable embedded systems resulting from various configuration schemes such as FPGA-like reconfiguration or dynamic voltage/frequency scaling (DVFS). We propose an intuitive heuristic to solve combined configuration selection and task scheduling problem on this graph. In addition, when applied to DVFS, our algorithm provides simultaneous task ordering and configuration selection of the system which outperforms the state-of-the-art DVFS methods applied after task ordering.
Extraction Thermodynamics of Polyanions into Plasticized Polymer Membranes Doped with Lipophilic Ion Exchangers: A Potentiometric Study (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExtraction Thermodynamics of Polyanions into Plasticized Polymer Membranes Doped with Lipophilic Ion Exchangers: A Potentiometric StudyBin Fu, Eric Bakker, Victor C. Yang, and Mark E. MeyerhoffCite this: Macromolecules 1995, 28, 17, 5834–5840Publication Date (Print):August 1, 1995Publication History Published online1 May 2002Published inissue 1 August 1995https://doi.org/10.1021/ma00121a021RIGHTS & PERMISSIONSArticle Views255Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alerts Get e-Alerts
Identification of Small Molecule Inhibitors of Pre-mRNA Splicing (2014)

Eukaryotic pre-mRNA splicing is an essential step in gene expression for all genes that contain introns. In contrast to transcription and translation, few well characterized chemical inhibitors are available with which to dissect the splicing process, particularly in cells. Therefore, the identification of specific small molecules that either inhibit or modify pre-mRNA splicing would be valuable for research and potentially also for therapeutic applications. We have screened a highly curated library of 71,504 drug-like small molecules using a high throughput in vitro splicing assay. This identified 10 new compounds that both inhibit pre-mRNA splicing in vitro and modify splicing of endogenous pre-mRNA in cells. One of these splicing modulators, DDD00107587 (termed "madrasin," i.e. 2-((7methoxy-4-methylquinazolin-2-yl)amino)-5,6-dimethylpyrimidin-4(3H)-one RNAsplicing inhibitor), was studied in more detail. Madrasin interferes with the early stages of spliceosome assembly and stalls spliceosome assembly at the A complex. Madrasin is cytotoxic at higher concentrations, although at lower concentrations it induces cell cycle arrest, promotes a specific reorganization of subnuclear protein localization, and modulates splicing of multiple pre-mRNAs in both HeLa and HEK293 cells.
Analytical computation of the RMS current stress on the DC-link capacitor in DC-link PWM converter systems powering N-phase rotating field machines in PWM and square wave mode (2004)

This paper describes an easy to handle analytical method for the computation of the rms current stress on the DC-link capacitor of PWM converters. Both the PWM and the square wave mode (SWM) operation powering rotating field machines e.g. induction- or synchronous machines are considered. The rms current stress on the DC-link capacitor mainly affects its working temperature. Therefore it is a significant factor for the estimation of its duty cycle. On the one hand the value of the DC-link capacitor current is determined by the amplitude and the phase angle of the inverter output phase current, deriving from the actual load. On the other hand it is influenced by the modulation depth and the phase number of the arrangement as well. The results achieved with the simple analytical model match very well with results of complex simulation models. The equivalent computing time compared to common simulation tools at reasonable time steps is reduced by more than a factor of thousand.
Noise masking in a transform domain (1993)

A.P. Varga and K.M. Ponting (1989) have demonstrated that the noise masking algorithm can provide effective noise compensation, leading to robust speech recognition with almost undegraded performance down to signal-to-noise ratios as low as 3 dB in stationary pink noise. Noise compensation techniques have been limited to the log energy spectral domain, whereas recognition is more normally carried out in a transform domain. The authors describe the adaptation of the noise masking algorithm for use in a cepstral transform domain, providing performance which is not significantly different from masking in the log energy domain. On the task examined here the technique compares favourably with the more computationally demanding hidden Markov model decomposition approach, with the performances diverging significantly only for signal-to-noise ratios worse than 3 dB. It can be seen that the use of noise masking can provide a good degree of noise robustness in both the filter bank domain and the mel scaled frequency cepstral domain.< <ETX>></ETX>
Variable Expression of Long QT Syndrome Among Gene Carriers from Families with Five Different HERG Mutations (2001)

The clinical expression of LQTS was equally variable in carriers from families with the same or different HERG mutations. These findings highlight the complexity of the clinical phenotype in this Mendelian dominant disorder and suggest that one or more modifier genes contribute to the variable expression of this syndrome.
Inhibitors of cancer cell multidrug resistance mediated by breast cancer resistance protein (BCRP/ABCG2) (2006)

Breast cancer resistance protein (BCRP/ABCG2) belongs to the ATP-binding cassette (ABC) transporter superfamily. It is able to efflux a broad range of anti-cancer drugs through the cellular membrane, thus limiting their anti-proliferative effects. Due to its relatively recent discovery in 1998, and in contrast to the other ABC transporters P-glycoprotein (MDR1/ABCB1) and multidrug resistance-associated protein (MRP1/ABCC1), only a few BCRP inhibitors have been reported. This review summarizes the known classes of inhibitors that are either specific for BCRP or also inhibit the other multidrug resistance ABC transporters. Information is presented on structure-activity relationship aspects and how modulators may interact with BCRP.
Effect of inhaled L-arginine on exhaled nitric oxide in normal and asthmatic subjects (1998)

An increase in the amount of substrate for NOS increases the formation of endogenous NO. L-arginine may have therapeutic potential in diseases in which there is defective production of NO, but in asthma it may amplify the inflammatory response in the airways.
SARS-CoV-2-Specific Memory T Lymphocytes From COVID-19 Convalescent Donors: Identification, Biobanking, and Large-Scale Production for Adoptive Cell Therapy (2021)

Syndrome coronavirus 2 (SARS-CoV-2) pandemic is causing a second outbreak significantly delaying the hope for the virus' complete eradication. In the absence of effective vaccines, we need effective treatments with low adverse effects that can treat hospitalized patients with COVID-19 disease. In this study, we determined the existence of SARS-CoV-2-specific T cells within CD45RA<sup>-</sup> memory T cells in the blood of convalescent donors. Memory T cells can respond quickly to infection and provide long-term immune protection to reduce the severity of COVID-19 symptoms. Also, CD45RA<sup>-</sup> memory T cells confer protection from other pathogens encountered by the donors throughout their life. It is of vital importance to resolve other secondary infections that usually develop in patients hospitalized with COVID-19. We found SARS-CoV-2-specific memory T cells in all of the CD45RA<sup>-</sup> subsets (CD3<sup>+</sup>, CD4<sup>+</sup>, and CD8<sup>+</sup>) and in the central memory and effector memory subpopulations. The procedure for obtaining these cells is feasible, easy to implement for small-scale manufacture, quick and cost-effective, involves minimal manipulation, and has no GMP requirements. This biobank of specific SARS-CoV-2 memory T cells would be immediately available "off-the-shelf" to treat moderate/severe cases of COVID-19, thereby increasing the therapeutic options available for these patients.
Analytical and clinical validation of a novel in-house deep-sequencing method for minimal residual disease monitoring in a phase II trial for multiple myeloma (2017)

support for the investigation of the contribution of altered ribosomal biogenesis to an oncogenic differentiation block.
Predicting Purchase Decisions in Mobile Free-to-Play Games (2015)

Mobile digital games are dominantly released under the freemium business model, but only a small fraction of the players makes any purchases. The ability to predict who will make a purchase enables optimization of marketing efforts, and tailoring customer relationship management to the specific user's profile. Here this challenge is addressed via two models for predicting purchasing players, using a 100,000 player dataset: 1) A classification model focused on predicting whether a purchase will occur or not. 2) a regression model focused on predicting the number of purchases a user will make. Both models are presented within a decision and regression tree framework for building rules that are actionable by companies. To the best of our knowledge, this is the first study investigating purchase decisions in freemium mobile products from a user behavior perspective and adopting behavior-driven learning approaches to this problem.