gebhart/patrepeval huggingface dataset:
Engineered endothelial progenitor cells that overexpress prostacyclin protect vascular cells (2011)

Prostacyclin (PGI2) is a potent vasodilator and important mediator of vascular homeostasis; however, its clinical use is limited because of its short (<2-min) half-life. Thus, we hypothesize that the use of engineered endothelial progenitor cells (EPCs) that constitutively secrete high levels of PGI2 may overcome this limitation of PGI2 therapy. A cDNA encoding COX-1-10aa-PGIS, which links human cyclooxygenase-1 (COX-1) to prostacyclin synthase (PGIS), was delivered via nucleofection into outgrowth EPCs derived from rat bone marrow mononuclear cells. PGI2-secreting strains (PGI2-EPCs) were established by continuous subculturing of transfected cells under G418 selection. Genomic PCR, RT-PCR, and Western blot analyses confirmed the overexpression of COX-1-10aa-PGIS in PGI2-EPCs. PGI2-EPCs secreted significantly higher levels of PGI2 in vitro than native EPCs (P < 0.05) and showed higher intrinsic angiogenic capability; conditioned medium (CM) from PGI2-EPCs promoted better tube formation than CM from native EPCs (P < 0.05). Cell- and paracrine-mediated in vitro angiogenesis was attenuated when COX-1-10aa-PGIS protein expression was knocked down. Whole-cell patch-clamp studies showed that 4-aminopyridine-sensitive K(+) current density was increased significantly in rat smooth muscle cells (rSMCs) cocultured under hypoxia with PGI2-EPCs (7.50 ± 1.59 pA/pF; P < 0.05) compared with rSMCs cocultured with native EPCs (3.99 ± 1.26 pA/pF). In conclusion, we successfully created EPC strains that overexpress an active novel enzyme resulting in consistent secretion of PGI2. PGI2-EPCs showed enhanced intrinsic proangiogenic properties and provided favorable paracrine-mediated cellular protections, including promoting in vitro angiogenesis of native EPCs and hyperpolarization of SMCs under hypoxia.
ChemInform Abstract: Mechanism of Reaction of Isomeric Nitrolic Acids to Nitrile Oxides in Aqueous Solution. (1991)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
An Overview of the Applications of Graphene‐Based Materials in Supercapacitors (2012)

Due to their unique 2D structure and outstanding intrinsic physical properties, such as extraordinarily high electrical conductivity and large surface area, graphene-based materials exhibit great potential for application in supercapacitors. In this review, the progress made so far for their applications in supercapacitors is reviewed, including electrochemical double-layer capacitors, pseudo-capacitors, and asymmetric supercapacitors. Compared with traditional electrode materials, graphene-based materials show some novel characteristics and mechanisms in the process of energy storage and release. Several key issues for improving the structure of graphene-based materials and for achieving better capacitor performance, along with the current outlook for the field, are also discussed.
Optical analysis of computed tomography images of the liver predicts fibrosis stage and distribution in chronic hepatitis C (2007)

Optical digital analysis of CT images of the liver is effective in determining the stage and distribution of liver fibrosis in chronic hepatitis C. In patients with homogeneous fibrosis distribution, the correlation between Fibro-CT and histology was better than in patients with heterogeneous distribution. Fibro-CT is a simple to use, readily available, and useful method for the diagnosis of fibrosis in patients with chronic hepatitis C.
Radiation-Induced Coloration of Photochromic Dithienylethene Derivatives (1999)

Abstract The radiation sensitivity of photochromic 1,2-dithienylethenes was studied with the aim to develop a new reusable color dosimeter. Upon γ-irradiation, crystalline colorless 1,2-bis(2,5-dimethyl-3-thienyl)perfluorocyclopentene turned red, and the absorption intensity increased linearly with the absorbed dose. The red color was bleached by visible-light irradiation and the crystal could be reused. On the other hand, crystalline 1,2-bis(2-methyl-1-benzothiophen-3-yl)perfluorocyclopentene did not show any radiation-induced coloration. The radiation sensitivity in solution was also examined to understand the possible applications to solution and film color dosimeters. Although radiation-induced coloration of the above two compounds was observed in benzene, no color change was detected in hexane, 2-methyltetrahydrofuran and n-butyl chloride. Excitation energy migration and transfer mechanism is considered to play a role in the coloration process in benzene.
An astatic magnetometer with negative feedback (1974)

A method for speeding up the response of an astatic magnetometer using negative feedback techniques is described. Closed loop stability is predicted using Bode's stability criterion for minimum phase elements. Accordingly, a design procedure is outlined for modifying the loop frequency response using operational amplifiers, which are readily available in integrated circuit form. The system is inexpensive to build and allows the deflection of an astatic magnetometer to be displayed on a digital voltmeter or a pen recorder over the entire useful range of the magnetometer, usually between 10-3 and 10-9 A m2.
All-optical signal processing at ultra-low powers in bottle microresonators using the Kerr effect (2010)

We present experimental results on nonlinear, ultra-low power photonics applications based on a silica whispering-gallery-mode microresonator. Our bottle microresonator combines an ultrahigh quality factor of Q > 10(8) with a small mode volume V. The resulting Q(2)/V-ratio is among the highest realized for optical microresonators and allows us to observe bistable behavior at very low powers. We report single-wavelength all-optical switching via the Kerr effect at a record-low threshold of 50 microW. Moreover, an advantageous mode geometry enables the coupling of two tapered fiber waveguides to a bottle mode in an add-drop configuration. This allows us to route a CW optical signal between both fiber outputs with high efficiency by varying its power level. Finally, we demonstrate that the same set-up can also be operated as an optical memory.
The novel transmembrane <i>Escherichia coli</i> proteins involved in the amino acid efflux (1999)

A novel gene of Escherichia coli, rhtB, has been characterized. Amplification of this gene provides resistance to homoserine and homoserine lactone. Another E. coli gene, rhtC, provides resistance to threonine. The homologues of RhtB are widely distributed among various eubacteria and archaea, from one to 12 copies of family members that differ in their primary structure were found in the genomes. Most of them are genes that encode hypothetical transmembrane proteins. Experimental data that indicate participation of the rhtB product in the excretion of homoserine have been obtained.
Purification and Properties of a Bacteriocin-Like Substance (Acnecin) of Oral <i>Propionibacterium acnes</i> (1978)

Propionibacterium acnes CN-8, isolated from human dental plaque, was grown in a liquid medium, and its bacteriocin-like substance (acnecin) was extracted from the cells by ultrasonic treatment. Acnecin was purified to a homogeneous state with recovery of 47%. Specific activity increased 72-fold in comparison with the crude extract. The properties of acnecin were as follows. (i) Acnecin may consist of five subunits with a molecular weight of about 12,000. (ii) Its isoelectric point was 5.5. (iii) In amino acid composition, aspartic acid, glutamic acid, glycine, and alanine were predominant, whereas cystine was not present. (iv) Acnecin contained 3.3% carbohydrate but was substantially free from lipid. (v) The activity was lost by heating at 60 degrees C or by protease and lysozyme treatments. Acnecin acted bacteriostatically on the indicator strain without killing it. The action spectrum of acnecin was very narrow; it was effective only against strains of non-acnecin-producing P. acnes and Corynebacterium parvum, a species closely related to P. acnes.
Rollback and Forking Detection for Trusted Execution Environments Using Lightweight Collective Memory (2017)

Novel hardware-aided trusted execution environments, as provided by Intel's Software Guard Extensions (SGX), enable to execute applications in a secure context that enforces confidentiality and integrity of the application state even when the host system is misbehaving. While this paves the way towards secure and trustworthy cloud computing, essential system support to protect persistent application state against rollback and forking attacks is missing. In this paper we present LCM - a lightweight protocol to establish a collective memory amongst all clients of a remote application to detect integrity and consistency violations. LCM enables the detection of rollback attacks against the remote application, enforces the consistency notion of fork-linearizability and notifies clients about operation stability. The protocol exploits the trusted execution environment, complements it with simple client-side operations, and maintains only small, constant storage at the clients. This simplifies the solution compared to previous approaches, where the clients had to verify all operations initiated by other clients. We have implemented LCM and demonstrated its advantages with a key-value store application. The evaluation shows that it introduces low network and computation overhead, in particular, a LCM-protected key-value store achieves 0.72x - 0.98x of an SGX-secured key-value store throughput.
Neuronal nicotinic alpha 7 receptor expressed in Xenopus oocytes presents five putative binding sites for methyllycaconitine. (1996)

1. The recently isolated compound methyllycaconitine (MLA) is a plant toxin which is a competitive inhibitor of nicotinic acetylcholine receptors (nAChRs). We found that homomeric alpha 7 receptors display a very high sensitivity to MLA with an IC50 in the picomolar range. 2. The competitive nature of the alpha 7 MLA blockade was reinforced by the observation that this compound has no action on wild-type serotoninergic receptors (5-HT3), whereas it is a powerful antagonist of chimaeric receptors alpha 7-5-HT3. 3. The time course of MLA inhibition of the wild-type (WT) alpha 7 follows a monotonic exponential decay whose time constant is proportional to the MLA concentration and could be described by a bimolecular mechanism with a forward rate constant (k+) of 2.7 x 10(7) S-1 M-1. In contrast, recovery from MLA inhibition displays an S-shaped time course that is incompatible with a simple bimolecular reaction. 4. Given the pentameric nature of the neuronal nicotinic receptors, a linear chain model, including five putative MLA binding sites corresponding to the homomeric nature of alpha 7, is proposed. 5. Both onset and recovery data obtained on the alpha 7 wild-type receptor are adequately described by this model assuming that a single MLA molecule is sufficient to block receptor function. 6. Analysis of MLA blockade and recovery of reconstituted heteromeric alpha 4 beta 2 receptors reveals, as expected, a time course compatible with only two binding sites for the toxin and, thus, further supports the validity of our model.
Mental Abilities of Children from Different Social-Class and Cultural Groups (1965)

Gerald S. Lesser, Gordon Fifer, Donald H. Clark, Mental Abilities of Children from Different Social-Class and Cultural Groups, Monographs of the Society for Research in Child Development, Vol. 30, No. 4, Mental Abilities of Children from Different Social-Class and Cultural Groups (1965), pp. 1-115
Hepatic B7 homolog 1 expression is essential for controlling cold ischemia/reperfusion injury after mouse liver transplantation (2011)

The study demonstrates that graft tissue expression of B7-H1 plays a critical role in regulating inflammatory responses during LT-induced hepatic I/R injury, and negative coregulatory signals may have an important function in hepatic innate immune responses.
Differences in the susceptibility of some penaeid prawn species to gill-associated virus (GAV) infection (2000)

Four species of penaeid prawn cultured in Australia (Penaeus monodon, Penaeus esculentus, Marsupenaeus japonicus and Fenneropenaeus merguiensis) were injected with a virulent preparation of gill-associated virus (GAV). P. monodon (average weight = 8.9, 13.9 and 19.2 g), P. esculentus (average weight = 19.5 g), F. merguiensis (average weight = 10.5 g), and small (average weight = 5.8 g) M. japonicus displayed overt signs of disease and mortalities which reached 82 to 100% within 23 d post-injection. Cumulative mortalities in P. esculentus and F. merguiensis were significantly lower than for P. monodon of the same size class. Medium (average weight = 13.0 g) M. japonicus also developed overt signs of disease but cumulative mortalities were not significantly higher than uninfected controls. Large (average weight = 20.3 g) M. japoncius did not display symptoms of disease and there were no significant mortalities up to 23 d post-injection.
Efficient method for the one-pot azidation of alcohols using bis(p-nitrophenyl) phosphorazidate (1997)

The direct stereoselective conversion of various alcohols and hexopyranoses into the corresponding alkyl azides and glycosyl azides, respectively, is efficiently accomplished by using bis(p-nitrophenyl) phosphorazidate and DBU.
17β-Estradiol/levonorgestrel transdermal system for the management of the symptomatic menopausal woman (2004)

Recent publications of the initial outcomes from the Women's Health Initiative (WHI) study of menopausal management have raised concerns over the safety of hormone therapy [1,2]. Every study, no matter how large or well conducted, has biases and limitations that preclude the ability to apply the outcomes to a larger group of individuals not specifically evaluated in the analysis. In particular, the hormonal arms of the WHI evaluated only a single dose of a daily oral regimen of conjugated equine oestrogen 0.625 mg [1,2], combined with medroxyprogesterone acetate 2.5 mg if the subject had a uterus [1]. The failure to evaluate non-oral regimens prevented the evaluation of hormone delivery systems that have been shown to provide similar symptom relief to oral regimens, but with a considerably different physiological impact. The once-weekly transdermal patch (Climara Pro releasing 17beta-estradiol 0.045 mg/day and levonorgestrel 0.015 mg/day has been shown to be highly effective in rapidly reducing the frequency and intensity of vasomotor symptoms and to significantly improve all categories in the quality of life Women's Health Questionnaire [3]. In addition, this transdermal combination system was not associated with any cases of endometrial hyperplasia, adverse impacts on cholesterol or lipid values and was associated with an increasing rate of amenorrhoea over time [3]. The 17beta-estradiol/levonorgestrel transdermal system is approved in the US for the treatment of moderate-to-severe vasomotor symptoms and the prevention and treatment of urogenital atrophy.
A simulation and training technique for analog neural network implementations (2002)

A neural network simulation technique tailored for analog hardware implementation is discussed. Lookup tables are used to represent the nonideal properties of the analog circuitry. A weight perturbation training algorithm, well suited for hardware implementations, is presented. Simulated annealing schemes are shown to improve training performance associated with analog neural networks.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Albumin-coated SPIONs: an experimental and theoretical evaluation of protein conformation, binding affinity and competition with serum proteins (2016)

The variety of nanoparticles (NPs) used in biological applications is increasing and the study of their interaction with biological media is becoming more important. Proteins are commonly the first biomolecules that NPs encounter when they interact with biological systems either in vitro or in vivo. Among NPs, super-paramagnetic iron oxide nanoparticles (SPIONs) show great promise for medicine. In this work, we study in detail the formation, composition, and structure of a monolayer of bovine serum albumin (BSA) on SPIONs. We determine, both by molecular simulations and experimentally, that ten molecules of BSA form a monolayer around the outside of the SPIONs and their binding strength to the SPIONs is about 3.5 × 10(-4) M, ten times higher than the adsorption of fetal bovine serum (FBS) on the same SPIONs. We elucidate a strong electrostatic interaction between BSA and the SPIONs, although the secondary structure of the protein is not affected. We present data that supports the strong binding of the BSA monolayer on SPIONs and the properties of the BSA layer as a protein-resistant coating. We believe that a complete understanding of the behavior and morphology of BSA-SPIONs and how the protein interacts with SPIONs is crucial for improving NP surface design and expanding the potential applications of SPIONs in nanomedicine.
The elasticity and rigidity of the outer coats of the eye (2008)

The elasticity of the porcine cornea and sclera could be approximated to constant over the physiological range, but showed a tendency to non-linearity. The modulus of elasticity for the sclera is 3 to 3.5 times that of the cornea. The cornea is more resistant than the sclera to IOP changes within the physiological range.
Thematic Role Based Generation of UML Models from Real World Requirements (2007)

Model-driven development depends on good initial models. Creating these models by hand is a challenging task, because of complex specification documents and change requests. We propose a new internal representation based on thematic roles, especially designed for (but not limited to) requirements documents. The representation can be generated automatically out of annotated real-world specification text and can be used to generate UML models based on graph rewriting rules.
Study of thermally induced vanadate dispersion (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudy of thermally induced vanadate dispersionD. Hoenicke and J. XuCite this: J. Phys. Chem. 1988, 92, 16, 4699–4702Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/j100327a028https://doi.org/10.1021/j100327a028research-articleACS PublicationsRequest reuse permissionsArticle Views69Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Identification of a methyl jasmonate‐responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain (1997)

A genomic DNA fragment was isolated containing 5' upstream sequences and part of the open reading frame corresponding to the lipoxygenase 1 cDNA (LoxA) expressed in barley grains during development and germination. Lox1 transcription was shown to be methyl jasmonate (MeJA)- and wound-inducible in leaves, but Lox1 transcripts were not detected in mildew-infected leaves, although this is a commonly observed response to pathogenic attack in various plants. Transient gene expression assays were used to identify a promoter region involved in MeJA-responsive expression. Analysis of 5' and 3' promoter deletions indicated that sequences between -363 and -294 conferred MeJA-responsive expression. Deletions/replacements covering this part of the promoter further defined a MeJA-responsive region between -331 and -291. Insertion of the region -328 to -293 into the constitutive CaMV 35S promoter conferred MeJA-responsive expression. The 36 bp fragment contains the motif TGACG as inverted repeats, which has been previously identified as a binding site for bZIP transactivating factors. Site-directed mutagenesis on these TGACG motifs abolished MeJA-responsive expression, clearly identifying them as MeJA-responsive elements. Sequence comparisons found no similar motif in other characterized promoters of MeJA-inducible genes, but suggested a common spatial structure which may serve as a binding site for transacting factors involved in the MeJA signal transduction pathway.
Millimeter-Wave and Submillimeter-Wave Imaging for Security and Surveillance Explosives hidden under clothing can be imaged by submillimeter waves, but millimeter waves are better suited for guiding helicopter navigation in poor weather. (2007)

Passive equipments operating in the 30-300 GHz (millimeter wave) band are compared to those in the 300 GHz- 3 THz (submillimeter band). Equipments operating in the submillimeter band can measure distance and also spectral information and have been used to address new opportunities in security. Solid state spectral information is available in the submillimeter region making it possible to identify materials, whereas in millimeter region bulk optical properties determine the image contrast. The optical properties in the region from 30 GHz to 3 THz are discussed for some typical inorganic and organic solids. In the millimeter-wave region of the spectrum, obscurants such as poor weather, dust, and smoke can be penetrated and useful imagery generated for surveillance. In the 30 GHz-3 THz region dielectrics such as plastic and cloth are also transparent and the detection of contraband hidden under clothing is possible. A passive millimeter-wave imaging concept based on a folded Schmidt camera has been devel- oped and applied to poor weather navigation and security. The optical design uses a rotating mirror and is folded using polarization techniques. The design is very well corrected over a wide field of view making it ideal for surveillance and security. This produces a relatively compact imager which minimizes the receiver count.
Regulation of Intracellular Ca2+ Stores by Multiple Ca2+-Releasing Messengers (2002)

Although glucose-elicited insulin secretion depends on Ca(2+) entry through voltage-gated Ca(2+) channels in the surface cell membrane of the pancreatic beta-cell, there is also ample evidence for an important role of intracellular Ca(2+) stores, particularly in relation to hormone- or neurotransmitter-induced insulin secretion. There is now direct evidence for Ca(2+) entry-induced release of Ca(2+) from the endoplasmic reticulum in neurons, but with regard to glucose stimulation of beta-cells, there is conflicting evidence about the operation of such a process. This finding suggests that the sensitivity of the Ca(2+) release channels in the endoplasmic reticulum membrane varies under different conditions and therefore is regulated. Recent evidence from studies of pancreatic acinar cells has revealed combinatorial roles of multiple messengers in setting the sensitivity of the endoplasmic reticulum for Ca(2+) release. Here we focus on the possible combinatorial roles of inositol 1,4,5-trisphosphate, cyclic ADP-ribose, and nicotinic acid adenine dinucleotide phosphate in beta-cell function.