gebhart/patrepeval huggingface dataset:
Comparison of the amino acid sequences of the transacylase components of branched chain oxoacid dehydrogenase of <i>Pseudomonas putida</i>, and the pyruvate and 2‐oxoglutarate dehydrogenases of <i>Escherichia coli</i> (1988)

The nucleotide sequence of bkdB, the structural gene for E2b, the transacylase component of branched-chain-oxoacid dehydrogenase of Pseudomonas putida has been determined and translated into its amino acid sequence. The start of bkdB was identified from the N-terminal sequence of E2b isolated from branched-chain-oxoacid dehydrogenase of the closely related species, P. aeruginosa. The reading frame was composed of 65.5% G + C with 82.3% of the codons ending in G or C. There was no intergenic space between bkdA2 and bkdB. No codons requiring minor tRNAs were utilized and the codon bias index indicated a preferential codon usage. The bkdB gene encoded 423 amino acids although the N-terminal methionine was absent from E2b prepared from P. aeruginosa. The relative molecular mass of the encoded protein was 45,134 (45,003 minus methionine) vs 47,000 obtained by SDS/polyacrylamide gel electrophoresis. There was a single lipoyl domain in E2b compared to three lipoyl domains in E2p, and one domain in E2o, the transacylases of pyruvate and 2-oxoglutarate dehydrogenases of Escherichia coli respectively. There was significant similarity between the lipoyl domain of E2b and of E2p and E2o as well as between the E1-E2 binding domains of E2b, E2p and E2o. There was no similarity between the E3 binding domain of E2b to E2p and E2o which may reflect the uniqueness of the E3 component of branched-chain-oxoacid dehydrogenase of P. putida. The conclusions drawn from these comparisons are that the transacylases of prokaryotic pyruvate, 2-oxoglutarate and branched-chain-oxoacid dehydrogenases descended from a common ancestral protein probably at about the same time.
A Machine Learning Framework for Predicting Purchase by Online Customers based on Dynamic Pricing (2014)

Pricing in the online world is highly transparent & can be a primary driver for online purchase. While dynamic pricing is not new & used by many to increase sales and margins, its benefit to online retailers is immense. The proposed study is a result of ongoing project that aims to develop a generic framework and applicable techniques by applying sound machine learning algorithms to enhance right price purchase (not cheapest price) by customers on e-commerce platform. This study focuses more on inventory led e-commerce companies, however the model can be extended to online marketplaces without inventories. Facilitated by statistical and machine learning models the study seeks to predict the purchase decisions based on adaptive or dynamic pricing of a product. Different data sources which capture visit attributes, visitor attributes, purchase history, web data, and context understanding, lays a strong foundation to this framework. The study focuses on customer segments for predicting purchase rather than on individual buyers. Personalization of adaptive pricing and purchase prediction will be the next logical extension of the study once the results for this are presented. Web mining and use of big data technologies along with machine learning algorithms make up the solution landscape for the study.
Thrombin and Lysophosphatidic Acid Receptors Utilize Distinct rhoGEFs in Prostate Cancer Cells (2004)

Thrombin and lysophosphatidic acid (LPA) receptors play important roles in vascular biology, development, and cancer. These receptors activate rho via G(12/13) family heterotrimeric G proteins, which are known to directly activate three distinct rho guanine nucleotide exchange factors (rhoGEFs) that contain a regulator of G protein signaling (RGS) domain (RGS-rhoGEFs). However, it is not known which, if any, of these RGS-rhoGEFs (LARG (leukemia-associated rhoGEF), p115rhoGEF, or PDZrhoGEF) plays a role in G protein-coupled receptor-stimulated rho signaling. Using oligonucleotide small interfering RNAs that suppress specific RGS-rhoGEF expression, we show that thrombin receptor stimulation of rho is primarily mediated by LARG in HEK293T and PC-3 prostate cancer cell lines. In contrast, the LPA-stimulated rho response in PC-3 cells is dependent on PDZrhoGEF expression. Suppression of p115rhoGEF had no effect. Thus different rhoGEFs (LARG and PDZrhoGEF) mediate downstream rho signaling by the thrombin and LPA receptors.
Reduction of the formation of ethylenethiourea from ethylenebis(dithiocarbamates) by cupric ions in aqueous media (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReduction of the formation of ethylenethiourea from ethylenebis(dithiocarbamates) by cupric ions in aqueous mediaSuzanne LesageCite this: J. Agric. Food Chem. 1980, 28, 4, 787–790Publication Date (Print):July 1, 1980Publication History Published online1 May 2002Published inissue 1 July 1980https://pubs.acs.org/doi/10.1021/jf60230a043https://doi.org/10.1021/jf60230a043research-articleACS PublicationsRequest reuse permissionsArticle Views65Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Hard carbon: a promising lithium-ion battery anode for high temperature applications with ionic electrolyte (2012)

Electrochemical behavior of a hard carbon plate in an electrolyte based on a room temperature ionic liquid consisting of trimethyl-n-hexylammonium (TMHA) cation and bis(trifluoromethanesulfone) imide (TFSI) anion was investigated. Hard carbon is found to be less prone to passivation due to the high electrochemical stability of the ionic liquid. Lithiation and de-lithiation of the carbon anode is strongly affected by temperature. At room temperature, the hard carbon is difficult to lithiate and a high potential hysteresis is observed between charge and discharge curves. Increasing temperature contributes to higher reversible capacity, higher coulombic efficiency, and lower potential hysteresis. At 80 °C, a reversible capacity of 16.2 mAh cm−2 (equivalent to 675.0 mAh g−1) was obtained with 73.6% of the first cycle coulombic efficiency. Considering that graphitic anodes do not work at this temperature due to the decomposition of the solid electrolyte interphase (SEI), the combination of hard carbon with ionic electrolyte can meet some special requirements for high temperature applications with improved safety. The activation energy for Li-ion transfer across the hard carbon/ionic electrolyte interface was measured by ac impedance spectroscopy; a value of 80 ± 5 kJ mol−1 was obtained. The large activation energy implies a high barrier of activation at the electrode/electrolyte interface, which accounts for the poor rate performance of the hard carbon anode in the ionic electrolyte.
Selective Dissolution of A‐Site Cations in ABO<sub>3</sub> Perovskites: A New Path to High‐Performance Catalysts (2015)

Selective dissolution is a common corrosion process in dealloying in which an alloy is immersed in acid to remove the active element, leaving behind an inert constituent. We introduce this technique into the treatment of oxide catalysts. A three-dimensionally ordered macroporous LaMnO3 perovskite has been prepared and treated with diluted HNO3 to selectively remove La cations, acquiring a novel γ-MnO2-like material. LaMnO3 is not a satisfactory catalyst on CO oxidation. Upon the removal of La cations, the obtained sample showed a significantly higher CO oxidation catalytic activity (T50=89 °C) than the initial precursor LaMnO3 (T50=237 °C) and ordinary γ-MnO2 (T50=148 °C). A large surface area, a high degree of mesoporosity, excellent low-temperature reducibility, and especially improved surface oxygen species are deduced to be responsible for CO oxidation at lower temperatures.
Placebo and Analgesic Nitrous Oxide for Treatment of the Alcohol Withdrawal State (1991)

We present evidence that analgesic (as distinct from anaesthetic) nitrous oxide is superior to placebo in the treatment of the alcohol withdrawal state. 'Carbogen' as a placebo is as effective as oxygen or medical air. However, placebo alone offers a means of treating the alcohol withdrawal state with a minimum of pharmacological intervention. Analgesic nitrous oxide for those not responding to placebo is a very rapid, effective, and extremely safe treatment which also reduces the use of highly addictive sedative pharmacological agents. This therapy also provides a rapid method of screening those patients requiring intensive monitoring and treatment in hospital from those who might benefit from out-patient therapy alone.
Constructing 3D CSG Models from 3D Raw Point Clouds (2018)

Abstract The Constructive Solid Geometry (CSG) tree, encoding the generative process of an object by a recursive compositional structure of bounded primitives, constitutes an important structural representation of 3D objects. Therefore, automatically recovering such a compositional structure from the raw point cloud of an object represents a high‐level reverse engineering problem, finding applications from structure and functionality analysis to creative redesign. We propose an effective method to construct CSG models and trees directly over raw point clouds. Specifically, a large number of hypothetical bounded primitive candidates are first extracted from raw scans, followed by a carefully designed pruning strategy. We then choose to approximate the target CSG model by the combination of a subset of these candidates with corresponding Boolean operations using a binary optimization technique, from which the corresponding CSG tree can be derived. Our method attempts to consider the minimal description length concept in the point cloud analysis setting, where the objective function is designed to minimize the construction error and complexity simultaneously. We demonstrate the effectiveness and robustness of our method with extensive experiments on real scan data with various complexities and styles.
Universal Streak Camera (1985)

A universal streak camera which works in synchroscan and single-shot modes with 3 plug-ins (synchroscan, fast and slow) has been developed utilizing a microchannel plate-incorporated streak tube. The synchroscan plug-in features low jitter of less than 4 ps, and high-speed sine-wave deflection from 80 to 160 MHz, achieving a limiting temporal resolution of 5 ps. The fast plug-in offers temporal resolution of better than 2 ps with triggering jitter of less than t20 ps. The slow plug-in provides longer time windows from 10 ns to 1 ms/15 mm in 16 ranges. Shutter operation down to 100 ns in duration is available with a maximum repetition rate of 1 - 10 KHz for the 3 plug-ins. An on-off ratio of more than 1:106 has been achieved by using double gate operation at photocathode and built-in microchannel plate.
Improved Quality of Awakening by Simulating Dawn Lighting with an Ordinary Ceiling Light (2001)

The effects of simulating dawn lighting with an ordinary ceiling light on the quality of awakening were investigated. The subjects were healthy men, aged 24-27. After sleep for about 7 hours with polysomnographic recordings in a climatic chamber kept at a temperature of 25t and a relative humidity of 50%, each subject was awakened by an alarm. For the 30 minutes immediately before the subject was awakened, the illumination in the chamber was gradually increased, simulating the condition of waking up as the sun rises. Alpha attenuation test, measuring blood pressure and awakening feelings were conducted after awakening.The time of sleep stage 2 appearing during the 30-minute simulating dawn lighting was significantly less (p<0.05; Wilcoxon test) than during typical waking conditions (i. e., waking up in the dark), while the time of stage W was higher (p=0.0796). The mood after awakening in the simulatig dawn lighting condition was significantly better (p<0.05). There were no significant differences in the alpha attenuation coefficients and blood pressures after awakening between the two conditions.These results suggest that simulating dawn lighting arouses subjects to light sleep, which makes awakening less sudden and more pleasant. There was no evidence that the light sleep immediately before awakening decreased the subjects' cerebral cortex activity or sympathetic nervous activity after awakening.
Human endothelial cells selectively express large amounts of pancreatic‐type ribonuclease (RNase 1) (2002)

Pyrimidine-specific ribonucleases are a superfamily of structurally related enzymes with distinct catalytic and biological properties. We used a combination of enzymatic and non-enzymatic assays to investigate the release of such enzymes by isolated cells in serum-free and serum-containing media. We found that human endothelial cells typically expressed large amounts of a pancreatic-type RNase that is related to, if not identical to, human pancreatic RNase. This enzyme exhibits pyrimidine-specific catalytic activity, with a marked preference for poly(C) substrate over poly(U) substrate. It was potently inhibited by placental RNase inhibitor, the selective pancreatic-type RNase inhibitor Inhibit-Ace, and a polyclonal antibody against human pancreatic RNase. The enzyme isolated from medium conditioned by immortalized umbilical vein endothelial cells (EA.hy926) possesses an amino-terminal sequence identical to that of pancreatic RNase, and shows molecular heterogeneity (molecular weights 18,000-26,000) due to different degrees of N-glycosylation. Endothelial cells from arteries, veins, and capillaries secreted up to 100 ng of this RNase daily per million cells, whereas levels were low or undetectable in media conditioned by other cell types examined. The corresponding messenger RNA was detected by RT-PCR in most cell types tested so far, and level of its expression was in keeping with the amounts of protein. The selective strong release of pancreatic-type RNase by endothelial cells suggests that it is endowed with non-digestive functions and involved in vascular homeostasis.
Intensive Statin Therapy: A Favorable Adjunct to the Improvement of Small-Diameter Vascular Grafts (2010)

To assess the effect of intensive statins therapy on the outcome of small-diameter vascular prosthesis, we investigated whether atorvastatin treatment (30 mg/d) could accelerate the re-endothelialization process and improve the patency rate in a canine infrarenal abdominal aorta-expanded polytetrafluoroethylene (ePTFE) bypass model. Furthermore, we also evaluated the effect of atorvastatin on the migratory and adherent capacity of circulating endothelial progenitor cells (EPCs) in vitro. Improved patency was confirmed by Doppler sonography and arteriography. Histological and scanning electron microscopy illustrated enhanced re-endothelialization process. Treatment with atorvastatin enhanced the circulating pool of EPCs with fortified migratory and adherent capacity. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis showed that atorvastatin treatment increased endothelial nitric oxide synthase (eNOS) and kinase insert domain receptor (KDR) messenger RNA (mRNA) expression in cultured EPCs and neointima. In conclusion, intensive statin therapy could be considered a favorable option to improve small-diameter vascular graft patency.
Molecular Connections between Cancer Cell Metabolism and the Tumor Microenvironment (2015)

Cancer cells preferentially utilize glycolysis, instead of oxidative phosphorylation, for metabolism even in the presence of oxygen. This phenomenon of aerobic glycolysis, referred to as the "Warburg effect", commonly exists in a variety of tumors. Recent studies further demonstrate that both genetic factors such as oncogenes and tumor suppressors and microenvironmental factors such as spatial hypoxia and acidosis can regulate the glycolytic metabolism of cancer cells. Reciprocally, altered cancer cell metabolism can modulate the tumor microenvironment which plays important roles in cancer cell somatic evolution, metastasis, and therapeutic response. In this article, we review the progression of current understandings on the molecular interaction between cancer cell metabolism and the tumor microenvironment. In addition, we discuss the implications of these interactions in cancer therapy and chemoprevention.
Endometrial Cancer: Biochemical and Clinical Correlates (1991)

Some endometrial cancers and endometrial adenocarcinoma cell lines show amplified expression of proto-oncogenes (fos, fms, myc, myb, neu, and erb-B) and augmented production of growth factors (colony-stimulating factor 1, epidermal growth factor, transforming growth factor-alpha, and transforming growth factor beta) and epidermal growth factor receptor. Oncogene expression, the presence of estrogen and progesterone receptors, and the fraction of cells in S phase are useful biochemical prognostic indicators of clinical outcome, and markers recognized by monoclonal antibodies are available for use in following the clinical course of the disease and responses to treatment. In vivo and in vitro studies on normal and neoplastic tissues are providing evidence of paracrine influences on epithelial cell proliferation. Long-term administration of tamoxifen as adjuvant therapy for breast cancer has recently been found to increase the risk for development of endometrial cancer.
Strategies in an era of global competition (2000)

The Australian government welcomed globalisation and led the way with tariff reductions, claiming that many local manufacturers were inefficient and inward looking as a result of over protection. This paper reports on a series of interviews conducted in companies belonging to the (small) electrical appliances and household kitchenware industries, which both face fierce competition from imported goods. The objectives were to find out what business and manufacturing strategies have sustained them in the new trade environment. Six distinct strategies were identified, although a mix is common. There has been a real push to increase productivity, but it is not enough on its own to enable a firm to defend its domestic market against cheap imports. The main impact of globalisation has been a move to replace local sources of production with others located offshore. Typically, these are independent suppliers in China operating under contract manufacturing agreements.
EmergencyGrid - Planning in Convergence Environments (2012)

Government agencies are often responsible for event handling, planning, coordination, and status reporting during emergency response in natural disaster events such as floods, tsunamis and earthquakes. Across such a range of emergency response scenarios, there is a common set of requirements that distributed intelligent computer systems generally address. To support the implementation of these requirements, some researchers are proposing the creation of grids, where final interface and processing nodes perform joint work supported by a network infrastructure. The aim of this project is to extend the concepts of emergency response grids, using a convergence scenario between web and other computational platforms. Our initial work focuses on the Interactive Digital TV platform, where we intend to transform individual TV devices into active final nodes, using a hierarchical planning structure. We describe the architecture of this approach and an initial prototype specification that is being developed to validate some concepts and illustrate the advantages of this convergence planning environment.
The functional logic of cortico-pulvinar connections (2003)

The pulvinar is an 'associative' thalamic nucleus, meaning that most of its input and output relationships are formed with the cerebral cortex. The function of this circuitry is little understood and its anatomy, though much investigated, is notably recondite. This is because pulvinar connection patterns disrespect the architectural subunits (anterior, medial, lateral and inferior pulvinar nuclei) that have been the traditional reference system. This article presents a simplified, global model of the organization of cortico-pulvinar connections so as to pursue their structure-function relationships.Connections between the cortex and pulvinar are topographically organized, and as a result the pulvinar contains a 'map' of the cortical sheet. However, the topography is very blurred. Hence the pulvinar connection zones of nearby cortical areas overlap, allowing indirect transcortical communication via the pulvinar. A general observation is that indirect cortico-pulvino-cortical circuits tend to mimic direct cortico-cortical pathways: this is termed 'the replication principle'. It is equally apt for certain pairs (or groups) of nearby cortical areas that happen not to connect with each other. The 'replication' of this non-connection is achieved by discontinuities and dislocations of the cortical topography within the pulvinar, such that the associated pair of connection zones do not overlap. Certain of these deformations can be used to divide the global cortical topography into specific sub-domains, which form the natural units of a connectional subdivision of the pulvinar. A substantial part of the pulvinar also expresses visual topography, reflecting visual maps in occipital cortex. There are just two well-ordered visual maps in the pulvinar, that both receive projections from area V l, and several other occipital areas; the resulting duplication of cortical topography means that each visual map also acts as a separate connection domain. In summary, the model identifies four topographically ordered connection domains, and reconciles the coexistence of visual and cortical maps in two of them. The replication principle operates at and below the level of domain structure.It is argued that cortico-pulvinar circuitry replicates the pattern of cortical circuitry but not its function, playing a more regulatory role instead. Thalamic neurons differ from cortical neurons in their inherent rhythmicity, and the pattern of cortico-thalamic connections must govern the formation of specific resonant circuits. The broad implication is that the pulvinar acts to coordinate cortical information processing by facilitating and sustaining the formation of synchronized trans-areal assemblies; a more pointed suggestion is that, owing to the considerable blurring of cortical topography in the pulvinar, rival cortical assemblies may be in competition to recruit thalamic elements in order to outlast each other in activity.