gebhart/patrepeval huggingface dataset:
Platelet-Derived Growth Factor A Chain Is Maternally Encoded in <i>Xenopus</i> Embryos (1988)

Transcription of zygotic genes does not occur in early Xenopus embryos until the mid-blastula transition, 6 to 7 hours after fertilization. Before this time, development is directed by maternal proteins and messenger RNAs stored within the egg. Two different forms of the A chain of platelet-derived growth factor (PDGF) are shown here to be encoded by maternal messenger RNAs. The two forms closely resemble human PDGF; however, the long form contains a hydrophobic region near the carboxyl terminus. The presence of PDGF messenger RNA in the embryo supports the idea that endogenous growth factors act at the earliest stages of embryogenesis.
Evaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agents (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvaluation of the relative effectiveness of different water-soluble .beta.-cyclodextrin media to function as fluorescence enhancement agentsRaymond P. Frankewich, K. N. Thimmaiah, and Willie L. HinzeCite this: Anal. Chem. 1991, 63, 24, 2924–2933Publication Date (Print):December 15, 1991Publication History Published online1 May 2002Published inissue 15 December 1991https://doi.org/10.1021/ac00024a023RIGHTS & PERMISSIONSArticle Views510Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alerts Get e-Alerts
Hand Motion-Based Remote Control Interface with Vibrotactile Feedback for Home Robots (2013)

This paper presents the design and implementation of a hand-held interface system for the locomotion control of home robots. A handheld controller is proposed to implement hand motion recognition and hand motion-based robot control. The handheld controller can provide a ‘connect-and-play’ service for the users to control the home robot with visual and vibrotactile feedback. Six natural hand gestures are defined for navigating the home robots. A three-axis accelerometer is used to detect the hand motions of the user. The recorded acceleration data are analysed and classified to corresponding control commands according to their characteristic curves. A vibration motor is used to provide vibrotactile feedback to the user when an improper operation is performed. The performances of the proposed hand motion-based interface and the traditional keyboard and mouse interface have been compared in robot navigation experiments. The experimental results of home robot navigation show that the success rate of the handheld controller is 13.33% higher than the PC based controller. The precision of the handheld controller is 15.4% more than that of the PC and the execution time is 24.7% less than the PC based controller. This means that the proposed hand motion-based interface is more efficient and flexible.
Synthesis of Bridgehead Nitrogen Heterocycles on a Solid Surface. (2005)

Abstract For Abstract see ChemInform Abstract in Full Text.
Entropy, entropy rate, and pattern classification as tools to typify complexity in short heart period variability series (2001)

An integrated approach to the complexity analysis of short heart period variability series (approximately 300 cardiac beats) is proposed and applied to healthy subjects during the sympathetic activation induced by head-up tilt and during the driving action produced by controlled respiration (10, 15, and 20 breaths/min, CR10, CR15, and CR20 respectively). The approach relies on: 1) the calculation of Shannon entropy (SE) of the distribution of patterns lasting three beats; 2) the calculation of a regularity index based on an entropy rate (i.e., the conditional entropy); 3) the classification of frequent deterministic patterns (FDPs) lasting three beats. A redundancy reduction criterion is proposed to group FDPs in four categories according to the number and type or of heart period changes: a) no variation (0V); b) one variation (1V); and c) two like variations (2LV); 4) two unlike variations (2UV). We found that: 1) the SE decreased during tilt due to the increased percentage of missing patterns; 2) the regularity index increased during tilt and CR10 as patterns followed each other according to a more repetitive scheme; and 3) during CR10, SE and regularity index were not redundant as the regularity index significantly decreased while SE remained unchanged. Concerning pattern analysis we found that: a) at rest mainly three classes (0V, 1V, and 2LV) were detected; b) 0V patterns were more likely during tilt; c) 1V and 2LV patterns were more frequent during CR10; and d) 2UV patterns were more likely during CR20. The proposed approach based on quantification of complexity allows a full characterization of heart period dynamics and the identification of experimental conditions known to differently perturb cardiovascular regulation.
Improvement of resilient packet ring fairness (2005)

Resilient packet ring (RPR, IEEE std. 802.17-2004) is a recent networking standard developed by the IEEE LAN/MAN working group. RPR is an insertion buffer, dual ring technology, utilizing a back pressure based fairness algorithm to distribute bandwidth when congestion occurs. In its attempt to control a set of nodes' sending behavior over a congested link, the RPR fairness algorithm suffers from two severe performance deficiencies. The first concerns how the node closest to a congested link calculates a fair rate estimate, the second deficiency relates to the method used to distribute this fair rate estimate to nodes upstream from the congested node. In this paper, we analyze these deficiencies and propose improvements to resolve them.
A Vectorless Approach for Predicting Switching Activity in a Digital Circuit (2016)

Existing vectorless methods use a statistical approach to estimate the average number of switching events in a digital circuit. While these methods allow one to predict the average power consumption, a critical parameter for many digital designs, they cannot be used to predict the statistical variation in power consumed in each clock cycle, which is necessary to estimate the bounds on power supply noise. A vectorless method is proposed for estimating the variance in the number of switching events across a digital design. This result may be used to estimate variance in power supply noise. The approach was tested on a 5 × 5 adder array. The standard deviation in the total number of switching events can be found within 1% for this design. Including covariance among switching events at different gates was critical. When covariance was ignored, the standard deviation was underestimated by nearly 84%. The proposed approach allows rapid estimation of a reasonable worst case number of switching events that may occur in a single clock cycle.
POSS Hybrid Robust Biomass IPN Hydrogels with Temperature Responsiveness (2019)

In order to improve the performance of traditional sodium alginate (SA) hydrogels cross-linked by Ca<sup>2+</sup> ions to meet greater application demand, a strategy was designed to structure novel SA-based gels (named OP-PN gels) to achieve both stimulus responsiveness and improved mechanical strength. In this strategy, the SA chains are co-cross-linked by CaCl₂ and cationic octa-ammonium polyhedral oligomeric silsesquioxane (Oa-POSS) particles as the first network, and an organically cross-linked poly(N-isopropyl acrylamide) (PNIPA) network is introduced into the gels as the second network. Several main results are obtained from the synthesis and characterization of the gels. For OP-PN gels, their properties depend on the content of both uniformly dispersed Oa-POSS and PNIPA network directly. The increased Oa-POSS and PNIPA network content significantly improves both the strength and resilience of gels. Relatively, the increased Oa-POSS is greatly beneficial to the modulus of gels, and the increased PNIPA network is more favorable to advancing the tensile deformation of gels. The gels with hydrophilic PNIPA network exhibit better swelling ability and remarkable temperature responsiveness, and their volume phase transition temperature can be adjusted by altering the content of Oa-POSS. The deswelling rate of gels increases gradually with the increase of POSS content due to the hydrophobic Si⁻O skeleton of POSS. Moreover, the enhanced drug loading and sustained release ability of the target drug bovine serum albumin displays great potential for this hybrid gel in the biomedical field.
Multiwavelength erbium-doped fibre laser basedon a high-birefringence fibre loop mirror (2000)

A novel multiwavelength fibre laser is proposed, which is based on a fibre loop mirror formed by a high-birefringence (HiBi) fibre, a polarisation controller, and a directional coupler. By setting the polarisation controller properly, the HiBi fibre loop mirror acts as a polarisation-independent wavelength filter. With this configuration, the wavelength spacing of the laser can be varied accurately by adjusting the length of the HiBi fibre. Experimentally, by cooling the erbium-doped fibre with liquid nitrogen to reduce its homogeneous linewidth, stable emission at 16 wavelengths with a spacing of 0.8 nm and an amplitude variation of < 5 dB was achieved.
Centralized Consensus Hemagglutinin Genes\nInduce Protective Immunity against H1, H3\nand H5 Influenza Viruses (2015)

With the exception of the live attenuated influenza vaccine there have been no substantial changes in influenza vaccine strategies since the 1940âs. Here we report an alternative vaccine approach that uses Adenovirus-vectored centralized hemagglutinin (HA) genes as vaccine antigens. Consensus H1-Con, H3-Con and H5-Con HA genes were computationally derived. Mice were immunized with Ad vaccines expressing the centralized genes individually. Groups of mice were vaccinated with 1 X 1010, 5 X 107 and 1 X 107 virus particles per mouse to represent high, intermediate and low doses, respectively. 100% of the mice that were vaccinated with the high dose vaccine were protected from heterologous lethal challenges within each subtype. In addition to 100% survival, there were no signs of weight loss and disease in 7 out of 8 groups of high dose vaccinated mice. Lower doses of vaccine showed a reduction of protection in a dose-dependent manner. However, even the lowest dose of vaccine provided significant levels of protection against the divergent influenza strains, especially considering the stringency of the challenge virus. In addition, we found that all doses of H5-Con vaccine were capable of providing complete protection against mortality when challenged with lethal doses of all 3 H5N1 influenza strains. This data demonstrates that centralized H1-Con, H3-Con and H5-Con genes can be effectively used to completely protect mice against many diverse strains of influenza. Therefore, we believe that these Ad-vectored centralized genes could be easily translated into new human vaccines.
The role of phospholipids in the stability of milk fat globules (1997)

This study examines the association between household air pollution from use of polluting cooking fuels and perinatal mortality in Bangladesh. We analysed the data from the Bangladesh Demographic and Health Surveys (BDHS) 2004, 2007, 2011, and 2014. The two outcome variables were stillbirth and early neonatal mortality. The exposure variable was type of primary cooking fuel used in the household (clean vs. polluting). Bivariate and multivariable analyses were conducted to obtain the crude and adjusted odds ratio (aOR), respectively. In the adjusted model, the exposure to polluting fuels was associated with early neonatal mortality (aOR: 1.46, 95% confidence interval [CI]: 1.01-2.10), but not with stillbirth (aOR: 1.25, 95% CI: 0.85-1.84). The effect of cooking with agricultural crop waste was greater for stillbirth (aOR: 1.76, 95% CI: 1.10-2.80) and for early neonatal mortality (aOR: 1.78, 95% CI: 1.13-2.80) which was also associated with wood as the main fuel (aOR: 1.52, 95% CI: 1.04-2.21). Using polluting fuels in an indoor kitchen was associated with an increased odds of stillbirth (aOR: 4.12, 95% CI: 1.49-11.41). Cooking with polluting fuels is associated with perinatal mortality. The combined association of polluting cooking fuels and indoor kitchen location was greater for stillbirth. This effect and the effect of different fuel types need further investigation. Although this is a large sample, there are some limitations with the BDHS data in both recording the exposure and the outcomes. A large prospective trial is needed to determine the precise effect size.
Purification of Human Plasma Kallikrein and Urokinase by Affinity Chromatography (1976)

L-Homoarginine benzylester was coupled with Sepharose 4B and used for affinity chromatography of human plasma kallikrein [EC 3.4.21.8] and urokinase [EC 3.4.99.26]. The euglobulin fraction of human plasma was treated with disodium ethylenediamine-tetraacetate (EDTA) and activated with acetone, and then kallikrein was purified 16-fold by affinity chromatography. The yield of kallikrein activity on affinity chromatography was 53%. The purified preparation of human plasma kallikrein appeared to be homogenous on gel electrophoresis. Commercial urokinase was also purified 4.8-fold with 64% yield by affinity chromatography.
New type inducers of differentiation of human HL-60 promyelocytic leukemia cells. Terephthalic anilides. (1984)

Induction of terminal differentiation of human myelogenous leukemia cells, HL-60, was caused by terephthalic acid mono-5, 5, 8, 8-tetramethyl-5, 6, 7, 8-tetrahydro-2-naphthylamide. The differentiated cells are morphologically and functionally granulocyte-like. The minimum concentration for detectable activity is less than 10-10M (0.04 ng/ml), and differentiation reaches a maximum at about 10-8-10-9M.
The cytoplasmic domain of tissue factor is phosphorylated by a protein kinase C-dependent mechanism. (1992)

Tissue factor (TF) is an integral membrane glycoprotein that serves as a cellular receptor and cofactor for the activation of the plasma protease factor VII. TF activity in both monocytes and endothelial cells is regulated by various cytokines and mitogens, including the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA). Three TF constructs (full-length human, a cytoplasmic domain deletion mutant, and a human-rat TF chimera), expressed in a human kidney cell line, were used to examine the in vivo phosphorylation state of TF after PMA treatment. The cytoplasmic domains of both rat and human TF were rapidly phosphorylated after cells were treated with 10-100 nM PMA. This response was completely abolished by preincubating cells with staurosporine, the potent PKC inhibitor, prior to PMA treatment. Localization of the phosphorylation site(s) to the cytoplasmic domain was demonstrated using a deletion mutant of TF and by CNBr digestion at the single methionine residue (Met-210) in the TF sequence. The rat TF cytoplasmic domain was phosphorylated to a higher specific activity than the human TF cytoplasmic domain. Phosphoamino acid analysis of the chimeric TF revealed both phosphothreonine and phosphoserine, whereas human TF contained only phosphoserine. Thus both potential phosphoacceptor sites are phosphorylated in the rat TF cytoplasmic domain. Alignment of TF cDNA sequences of mouse, rat, rabbit, and man revealed that the phosphoacceptor site (X-S*/T*-P-X, where asterisk indicates the phosphorylated residue) in the cytoplasmic domain has been conserved through evolution.
Combi-sensor for Oil Level and Oil Quality Management (2008)

<div class="htmlview paragraph">For modern oil management systems in vehicles we developed an innovative combi-sensor. The sensor component is capable of measuring 6 independent oil parameters (viscosity, density, permittivity, conductance, level, temperature) continously during normal engine operation. It comprises an ultrasonic transducer for the oil level detection as well as a Tuning Fork mechanical resonator for the oil condition measurement. Both elements have been realized as small multi-chip modules, which are combined within a mechanical flange. All data is transmitted to an external ECU by a 1-wire digital interface protocol.</div> <div class="htmlview paragraph">In addition to the measurement and signal preconditioning software that is already implemented on the system, an AUTOSAR-conform software module will be provided for the customer. This software evaluates the incoming signals from the sensor and calculates the current oil quality, considering additional information from the engine. The intelligent Oil Condition Algorithm (OCA) detects contaminants and extreme oil conditions at an early stage. In this way oil change intervals can be adapted to the specific car and the individual driving behavior in a more precise way than is possible with existing oil condition monitoring concepts and algorithms.</div> <div class="htmlview paragraph">Since the oil level can be measured even when the engine is stopped, the sensor ensures an overfill detection comparable to a regular dipstick, which is then redundant.</div>
N-Terminal Modification of Proteins with <i>o</i>-Aminophenols (2014)

The synthetic modification of proteins plays an important role in chemical biology and biomaterials science. These fields provide a constant need for chemical tools that can introduce new functionality in specific locations on protein surfaces. In this work, an oxidative strategy is demonstrated for the efficient modification of N-terminal residues on peptides and N-terminal proline residues on proteins. The strategy uses o-aminophenols or o-catechols that are oxidized to active coupling species in situ using potassium ferricyanide. Peptide screening results have revealed that many N-terminal amino acids can participate in this reaction, and that proline residues are particularly reactive. When applied to protein substrates, the reaction shows a stronger requirement for the proline group. Key advantages of the reaction include its fast second-order kinetics and ability to achieve site-selective modification in a single step using low concentrations of reagent. Although free cysteines are also modified by the coupling reaction, they can be protected through disulfide formation and then liberated after N-terminal coupling is complete. This allows access to doubly functionalized bioconjugates that can be difficult to access using other methods.
Certificate revocation the responsible way (2002)

Public-key certificates are managed by a combination of the informal web of trust and the use of servers maintained by organizations. Prompt and reliable distribution of revocation notices is an essential ingredient for security in a public-key infrastructure. Current schemes based on certificate revocation lists on key servers are inadequate. An approach based on distributing revocation notices to "dependers" on each certificate, with cascading forwarding, is suggested. Research is necessary to investigate architectural issues, particularly reliability and response time analysis.
Rhodococcus equi foal pneumonia: Protective effects of immune plasma in experimentally infected foals (1989)

The immunoprophylactic capacity of specific immune plasma was evaluated in pony foals infected experimentally with Rhodococcus equi. Immune plasma, produced by repeated parenteral administration of viable R. equi to adult horses, was harvested and frozen. Group I (six control foals) and Group II (six principal foals) received lactated Ringers solution and immune plasma respectively at three and five days of age. R. equi were aerosolised into a caudal lung lobe of all foals at seven days of age. Clinical signs, haematological alterations, immune responses, thoracic radiographs and technetium99m pulmonary perfusion scans were monitored. All foals were destroyed and complete post mortem examinations performed. All foals developed pneumonia as evidenced by clinical, radiographic and perfusion alterations, but the survival rate of principal foals was significantly (P less than 0.01) greater than that of control foals. Five control foals developed terminal disease, whereas all principal foals recovered. There was no significant (P greater than 0.05) difference in temperature response, or peripheral blood leucocyte, neutrophil or fibrinogen concentrations between groups. ELISA values for R. equi antibody were significantly (P less than 0.001) greater in principal foals following treatment, but there was no significant (P greater than 0.05) difference in IgG or IgM concentrations between groups. Results of the haemolysis inhibition assay indicated that equi factor neutralising antibodies were transferred by immune plasma to the principal foals. Post mortem examinations of five control foals destroyed at approximately three weeks post infection because of terminal disease, revealed severe pyogranulomatous pneumonia. One control and all principal foals were either free of lesions or had resolving lesions and/or minimal scar formation at three months post infection.(ABSTRACT TRUNCATED AT 250 WORDS)
Adapting the Virtual Platooning Concept to Roundabout Crossing (2018)

Despite the tremendous growth of research regarding fully autonomous vehicles in the past few years, many safety critical scenarios, such as the crossing of roundabouts, are still open issues. One of the main challenge is to deal with the lack of visibility in complex environments. Vehicle-to-vehicle and vehicle-to-infrastructure communications offer an appealing solution to handle these situations in a cooperative way. To avoid computing an ad hoc control strategy for every possible scenario, we propose in this paper to adapt the concept of virtual platooning to roundabout crossing. This idea allows a single platooning control law to handle complex scenarios such as intersection and roundabout crossings. This work combines the use of high definition maps and a curvilinear coordinates framework to deal with any kind of roundabouts. The proposed approach is not limited to communicating autonomous vehicles but can also be used with manually driven communicating vehicles or non-communicating vehicles with the help on the infrastructure. A formal proof of the correctness of this approach is given and simulations have been carried out with a high definition map of a real roundabout. We also introduce a novel graphical representation called safety diagram to study de performances of our approach.
[Determination of chromosome of Gluconobacter oxydans SCB329]. (2001)

After the pure culture of Gluconobacter oxydans SCB329 was researched, its growth curve was measured and its logarithmic phase was determined as between 4-24 h. After the microorganisms were havested in its logarithmic phase, The intact chromosome was prepared by agarose-embedded method. Then the genome of SCB329 was analyzed by Pulsed-field Gel Electrophoresis. The result indicated that there are one chromosome and one great plasmid. The length of intact chromosome of SCB329 has been estimated to be approximately between 2.2 Mb and 3.5 Mb.