gebhart/patrepeval huggingface dataset:
Toward Continuous Monitoring of Breath Biochemistry: A Paper-Based Wearable Sensor for Real-Time Hydrogen Peroxide Measurement in Simulated Breath (2019)

Exhaled breath contains a large amount of biochemical and physiological information concerning one's health and provides an alternative route to noninvasive medical diagnosis of diseases. In the case of lung diseases, hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is an important biomarker associated with asthma, chronic obstructive pulmonary disease, and lung cancer and can be detected in exhaled breath. The current method of breath analysis involves condensation of exhaled breath, is not continuous or real time, and requires two separate and bulky devices, complicating the periodic or long-term monitoring of a patient. We report the first disposable paper-based electrochemical wearable sensor that can monitor exhaled H<sub>2</sub>O<sub>2</sub> in artificial breath calibration-free and continuously, in real time, and can be integrated into a commercial respiratory mask for on-site testing of exhaled breath. To improve precision for sensing H<sub>2</sub>O<sub>2</sub>, we perform differential electrochemical measurement by amperometry in which screen-printed Prussian Blue-mediated and nonmediated carbon electrodes are used for differential analysis. We were able to measure H<sub>2</sub>O<sub>2</sub> in simulated breath in a concentration-dependent manner in real time, confirming its functionality. This proposed system is versatile, and by modifying the chemistry of the sensing electrodes, our method of differential sensing can be extended to continuous monitoring of other analytes in exhaled breath.
Chemical deodorization of the foul smelling sulfur compounds. (1996)

Though the foul smelling sulfur compounds in pulp drainage (hydrogen sulfide, methyl mercaptan, methyl sulfide and dimethyl disulfide) have been usually deodorized by the air-or steam- stripping method, methyl sulfide and dimethyl disulfide are liable to remain in the effluents.In this study, we examined the alternative chemical method using chemicals which react with the sulfur containing compounds and noticed that iron/hydrogen peroxide system is effective for removing not only hydrogen sulfide and methyl mercaptan but also methyl sulfide and dimethyl disulfide.We found, furthermore, that a vanadium compound enhanced remarkably the performance of this system.
Recursive Hierarchical Image Segmentation by Region Growing and Constrained Spectral Clustering (2002)

This paper describes an algorithm for hierarchical image segmentation (referred to as HSEG) and its recursive formulation (referred to as RHSEG). The HSEG algorithm is a hybrid of region growing and constrained spectral clustering that produces a hierarchical set of image segmentations based on detected convergence points. In the main, HSEG employs the hierarchical stepwise optimization (HS WO) approach to region growing, which seeks to produce segmentations that are more optimized than those produced by more classic approaches to region growing. In addition, HSEG optionally interjects between HSWO region growing iterations merges between spatially non-adjacent regions (i.e., spectrally based merging or clustering) constrained by a threshold derived from the previous HSWO region growing iteration. While the addition of constrained spectral clustering improves the segmentation results, especially for larger images, it also significantly increases HSEG's computational requirements. To counteract this, a computationally efficient recursive, divide-and-conquer, implementation of HSEG (RHSEG) has been devised and is described herein. Included in this description is special code that is required to avoid processing artifacts caused by RHSEG s recursive subdivision of the image data. Implementations for single processor and for multiple processor computer systems are described. Results with Landsat TM data are included comparing HSEG with classic region growing. Finally, an application to image information mining and knowledge discovery is discussed.
Combined A<sup>3</sup> Coupling and Click Chemistry Approach for the Synthesis of Dendrimer-Based Biological Tools (2014)

We report a versatile approach in which two highly efficient chemical reactions, multicomponent A<sup>3</sup> coupling and alkyne-azide click chemistry, are combined to construct dendrimer-based tools for applications in biology. Using a convergent approach, dendrons with desired architecture and an alkyne at the focal point are first assembled and then stitched together via multicomponent A<sup>3</sup> coupling reaction. The desired functional groups, including a stealth agent, imaging dye, and drug molecules, could be easily covalently linked to the surfaces of these hyperbranched macromolecules using alkyne-azide click chemistry. These A<sup>3</sup>-click dendrimers are noncytotoxic at concentrations as high as 1 μM and in fact reduce the toxicity of the drug. The dye-coated dendrimers specifically target and localize in lipid droplets. This unison methodology represents an attractive chemical strategy in exploiting the untapped potential of A<sup>3</sup> coupling and facilitating the development of nanodevices for imaging and drug delivery.
Enumeration of Absolute Numbers of T and B Lymphocytes in Human Blood (1974)

A new immunofluorescence test permits the direct enumeration of T and B lymphocytes in unseparated blood. B cells were identified with antiimmunoglobulin sera and T cells by a rabbit anti‐human brain serum rendered unreactive with B cells by sequential absorption with erythrocytes, liver, and either chronic lymphocytic leukaemia cells or a cell line, BRI‐8, with B‐cell surface characteristics. T and B cells were also enumerated with lymphocyte suspension purified by either Ficoll‐lsopaque density sedimentation or by a two‐step gelatin‐polystyrene bead column method. T cells in Ficoll‐purified suspension were also enumerated by rosette formation with sheep erythrocytes. Comparison of the T‐B proportions evaluated with the different cell preparation showed that the cell separation procedures can give rise to a deviation in the T:B ratio in favour of B cells. The extent of this induced bias related inversely to lymphocyte yield. Staining of whole blood preparations or high‐yield purified cells enables absolute numbers of T and B lymphocytes to be calculated. We consider this measurement considerably more informative than relative proportions of T and B cells.
Construction of causality diagram model for diagnostics (2008)

Causality diagram is one of the most effective theoretical models for uncertainty knowledge expression and reasoning. In order to realize the computer-aided construction of the causality diagram for diagnostics, this paper proposes a simple, yet comprehensive representation model to organize the failure knowledge more systematically and completely. This method defines the failure modes as the inherent properties of the physical entities at different hierarchical levels, and employs the polychromatic sets theory to represent the failure modes in terms of their interrelationships and their relations to the physical system. Then, the infrastructure of the diagnostic causality diagram (DCD) is constructed in a deductive manner by using the iterative search process operated on the reasoning matrices of the polychromatic sets. A case study is used to illustrate the feasibility of the proposed method. The research shows that the polychromatic sets approach to the DCD construction has formed mathematical foundation and can be readily implemented in computer.
Detection of Tumor-Associated Antigens in Urine from Patients with Bladder Cancer (1980)

An antiserum was prepared to a hypotonic saline extract of bladder tumor tissue which, after absorption with normal tissues, was used to localize tumor-associated antigens in a Sephadex G-150 elution profile of urine from patients with bladder cancer. A second antiserum was prepared to the urine fraction with tumor-associated antigens. After absorption with normal urine and plasma this antiserum was reacted in gel diffusion and complement fixation assays against urine samples from normal individuals, patients with bladder cancer and patients with proved urinary tract infections. In the gel diffusion assays none of the 23 urine specimens from normal individuals, 37 per cent of the 24 urine specimens from patients with urinary tract infections, 61 per cent of 13 urine samples from patients with papillomas and in situ tumors, and 95 per cent of 30 samples from patients with bladder cancer gave positive precipitin reactions. In complement fixation assays urine from 53 patients with papillomas through grade IV cancers showed a mean 50 per cent complement fixation at 30 micrograms. protein, 29 patients with urinary tract infections showed a mean 50 per cent complement fixation at 150 micrograms. protein and all but 6 urine specimens from more than 30 normal individuals showed 50 per cent complement fixation (mean value) at 140 micrograms. protein. Thus, an antiserum has been produced that may be used in assays for the diagnosis of bladder cancer.
Factors affecting L-lactate utilization by Selenomonas ruminantium (1994)

Studies were conducted to evaluate factors that affect L-lactate utilization by the ruminal bacterium Selenomonas ruminantium HD4. L-Lactate uptake decreased over time both in the presence and absence of 10 mM L-malate. Compared with uptake in the absence of malate, 10 mM L-malate increased L-lactate uptake at 30 s and 45 min. Because L-malate had little effect on L-lactate uptake for cells grown on soluble carbohydrates compared to lactate-grown cells, it seems that the stimulation due to L-malate is inducible. Sodium concentrations between 25 and 100 mM stimulated long-term (45 min) L-lactate uptake in the presence of 10 mM L-malate, whereas uptake in the absence of L-malate was low regardless of the Na+ concentration. Monensin inhibited L-lactate uptake by 42%, and this is consistent with the involvement of Na+ in L-lactate utilization. Initial uptake (30 s) of L-lactate was not dependent on Na+. L-Lactate uptake was stimulated by 10 mM L-malate at extracellular pH values between 4.0 and 8.0. No inhibition of L-lactate uptake was observed in the presence of 10 mM glucose, maltose, sucrose, xylose, or D-lactate. The metabolic inhibitors carbonyl cyanide m-chlorophenyl-hydrazone, 2,4-dinitrophenol, and NaF inhibited L-lactate uptake (> 79%), suggesting that protons may be involved in L-lactate uptake by this bacterium. Collectively, these experiments show that L-lactate uptake by S. ruminantium HD4 is stimulated in the presence of 10 mM L-malate at pH values and Na+ concentrations commonly found in the rumen.
Cloning and characterization of the genes encoding the<i>Msp</i>l restriction modification system (1989)

The genes encoding the MspI restriction modification system, which recognizes the sequence 5' CCGG, have been cloned into pUC9. Selection was based on expression of the cloned methylase gene which renders plasmid DNA insensitive to MspI cleavage in vitro. Initially, an insert of 15 kb was obtained which, upon subcloning, yielded a 3 kb EcoRI to HindIII insert, carrying the genes for both the methylase and the restriction enzyme. This insert has been sequenced. Based upon the sequence, together with appropriate subclones, it is shown that the two genes are transcribed divergently with the methylase gene encoding a polypeptide of 418 amino acids, while the restriction enzyme is composed of 262 amino acids. Comparison of the sequence of the MspI methylase with other cytosine methylases shows a striking degree of similarity. Especially noteworthy is the high degree of similarity with the HhaI and EcoRII methylases.
A Comparison of Laterally Condensed Gutta-Percha, Thermoplasticized Gutta-Percha, and Mineral Trioxide Aggregate as Root Canal Filling Materials (2004)

The purpose of this study was to evaluate the potential of using mineral trioxide aggregate as a root canal filling material by comparing its apical sealing ability with that of laterally condensed gutta-percha with sealer and high-temperature thermoplasticized gutta-percha with sealer in extracted bovine teeth. Sixty bovine incisors with single canals were prepared in a standard manner using LightSpeed instruments, randomly divided into three groups of 20 teeth, and obturated. The sealing ability of each technique was assessed by immersion in 1% methylene blue dye for 3 days. The teeth were cleared, and the linear extent of dye penetration was measured with a stereomicroscope. Data were analyzed by the Kruskal-Wallis one-way ANOVA followed by Dunn's test. Canals filled with laterally condensed gutta-percha or thermoplasticized gutta-percha showed significantly less apical dye penetration than canals obturated with mineral trioxide aggregate (p < 0.001). There was no significant difference in leakage between the laterally condensed group and the thermoplasticized group. The results suggest that gutta-percha obturation may provide an apical seal that is superior to MTA.
Cyclosporin A‐induced free radical generation is not mediated by cytochrome P‐450 (2002)

1. Reactive oxygen species (ROS) have been proposed to play a role in the side effects of the immunosuppressive drug cyclosporin A (CsA). 2. The aim of this study was to investigate whether cytochrome P-450 (CYP) dependent metabolism of CsA could be responsible for ROS generation since it has been suggested that CsA may influence the CYP system to produce ROS. 3. We show that CsA (1 -- 10 microM) generated antioxidant-inhibitable ROS in rat aortic smooth muscle cells (RASMC) using the fluorescent probe 2,7-dichlorofluorescin diacetate. 4. Using cytochrome c as substrate, we show that CsA (10 microM) did not inhibit NADPH cytochrome P-450 reductase in microsomes prepared from rat liver, kidney or RASMC. 5. CsA (10 microM) did not uncouple the electron flow from NADPH via NADPH cytochrome P-450 reductase to the CYP enzymes because CsA did not inhibit the metabolism of substrates selective for several CYP enzymes that do not metabolize CsA in rat liver microsomes. 6. CsA (10 microM) did not generate more radicals in CYP 3A4 expressing immortalized human liver epithelial cells (T5-3A4 cells) than in control cells that do not express CYP 3A4. 7. Neither diphenylene iodonium nor the CYP 3A inhibitor ketoconazole were able to block ROS formation in rat aortic smooth muscle or T5-3A4 cells. 8. These results demonstrate that CYP enzymes do not contribute to CsA-induced ROS formation and that CsA neither inhibits NADPH cytochrome P-450 reductase nor the electron transfer to the CYP enzymes.
Coliform--E. coli (2009)

Cereals & Grains Association is a nonprofit organization dedicated to advancing the knowledge and understanding of cereal grain science through research leadership, education, superior technical service, and advocacy.
Comparative Evaluation of Filters Used in Membrane Plasmapheresis (1984)

Protocols were developed for in vivo and in vitro characterization of the mass transfer performance of filters intended for use in membrane plasmapheresis. The protocols were applied to all presently available filters and also to secondary filters used in cascade filtration. Virtually no distinction was found in filtration rate or sieving coefficient of the ten plasma filters tested and all, except for one early model now considered obsolete, are clinically equivalent. In contrast, filters for cascade filtration varied widely in performance and still require further development for optimal use.
Security Measures for Black Hole Attack in MANET: An Approach (2012)

A Mobile Ad-Hoc Network is a collection of mobile nodes that are dynamically and arbitrarily located in such a manner that the interconnections between nodes are capable of changing on continual basis. Due to security vulnerabilities of the routing protocols, wireless ad-hoc networks are unprotected to attacks of the malicious nodes. One of these attacks is the Black Hole Attack. In this paper, we give an algorithmic approach to focus on analyzing and improving the security of AODV, which is one of the popular routing protocols for MANET. Our aim is on ensuring the security against Black hole attack. The proposed solution is capable of detecting & removing Black hole node(s) in the MANET at the beginning. Also the objective of this paper is to provide a simulation study that illustrates the effects of Black hole attack on network performance.
Mannopeptimycins, Novel Antibacterial Glycopeptides from <i>Streptomyces </i><i>h</i><i>ygroscopicus</i>, LL-AC98 (2002)

A series of novel antibiotics with activity against methicillin-resistant staphylococci and vancomycin-resistant enterococci has been purified, and their structures have been characterized using spectroscopic analyses and chemical conversions. These antibiotics, designated mannopeptimycins alpha-epsilon (1-5), are glycosylated cyclic hexapeptides containing two stereoisomers of an unprecedented amino acid, alpha-amino-beta-[4'-(2'-iminoimidazolidinyl)]-beta-hydroxypropionic acid (Aiha), as a distinguishing feature. The cyclic peptide core of these antibiotics is attached to a mannosyl monosaccharide moiety in 2 and to mannosyl monosaccharide and disaccharide moieties in 1, 3, 4, and 5. The presence and position of an isovaleryl group in the terminal mannose (Man-B) in 3-5 are critical for retaining antibacterial potency.
Interim results of a randomized trial of mitomycin C as an adjunct to radical radiotherapy in the treatment of locally advanced squamous-cell carcinoma of the cervix (2000)

The purpose of this study was to determine the efficacy of mitomycin C as an adjunct to radiotherapy for the treatment of locally advanced cervix cancer. Patients with squamous-cell carcinoma of the cervix, stages IB2-IVA, were randomized to receive radiotherapy alone or radiotherapy with concomitant mitomycin C. An initial cohort of 160 patients, having a mean follow-up of 46 months, is analyzed. Intravenous mitomycin C, 15 mg/M(2), was given on the first and sixth week of radiotherapy. The 78 patients in the radiotherapy with mitomycin C group and 82 patients in the radiotherapy alone group have a comparable distribution by age and stage (mean age 47 years; stage IB 3%, IIA 11%, IIB 48%, IIIA 1%, IIIB 36%, IVA 3%). The four-year actuarial survival rates for radiotherapy with mitomycin C and radiotherapy alone were 72% and 56%, respectively (P = 0.13). The four-year actuarial disease-free survival rates for radiotherapy with mitomycin C and radiotherapy alone were 71% and 44%, respectively, a statistically significant difference (P = 0.01). The four-year actuarial local recurrence-free survival rates for patients receiving radiotherapy with mitomycin C and radiotherapy alone were 78% and 63%, respectively (P = 0.11). Differences in four-year distant recurrence-free survival between radiotherapy plus mitomycin C and radiotherapy alone were significantly different at 85% vs. 61% (P = 0.01); this analysis is not adjusted for local failure. On subgroup analysis, stage III-IVA patients had a four-year actuarial disease-free survival of 75% for radiotherapy plus mitomycin C compared with 35% for radiotherapy alone (P = 0.03). There were no treatment- related deaths. Mild hematologic toxicity was seen only in the group treated with mitomycin C. No excess in non-hematologic toxicity has been observed thus far with combined mitomycin C and radiotherapy. In this open phase III trial of mitomycin C as an adjunct to radical radiotherapy for squamous-cell carcinoma of the cervix, there were minimal hematologic effects and no increase in acute radiation reactions. A statistically significant difference in favor of patients receiving mitomycin C is shown for disease-free survival. Thus far, there are trends in favor of those patients receiving mitomycin C for survival and local control. Patients with more advanced stage disease, predominantly stage IIIB, appear to have the most benefit. These preliminary results support the hypothesis that targeting hypoxic cells may lead to a therapeutic enhancement in the radiotherapy of cervix cancer. This trial continues to accrue patients and follow-up data. Int. J. Cancer (Radiat. Oncol. Invest.) 90, 206-223 (2000).
Metamaterial millimeter wave directional coupler on silicon substrate (2010)

This paper presents the design and manufacturing of a metamaterial Composite Right/Left Handed (CRLH) directional coupler on silicon substrate in coplanar waveguide (CPW) configuration, with a frequency band between 26-28 GHz. The design makes use of 4 CRLH cells, cascaded and coupled two by two in order to obtain the directional coupler structure. The device was processed on a 500μm thick silicon substrate with a Au/Cr metallization with the purpose of easy integration in more complex millimeter wave integrated circuits. The measurement of working frequency, reflection loss and isolation of the coupler validates the calculated values of these parameters.
Signaling Pathways Responsible for Cancer Cell Invasion as Targets for Cancer Therapy (2004)

Migration of cancer cells is one of the key factors responsible for cancer metastasis. The elucidation of mechanisms responsible for the highly invasive potential of cancer cells can help to identify specific targets for the treatment of cancer patients. Highly invasive cancers are usually characterized by aberrant activity of specific intra- or extracellular molecules such as protein kinases, phosphatases, transcriptional factors, proteolytic enzymes, and others. Protein kinase C (PKC) and phosphatidylinositol 3-kinase (PI3K) are responsible for the constitutive activity of transcriptional factors NF-kappaB and AP-1 in some of the highly invasive cancers. Furthermore, NF-kappaB and AP-1 control the expression of urokinase-type plasminogen activator (uPA) and its receptor (uPAR), and expression of both uPA and uPAR correlates with invasive cancer cell phenotype and poor prognosis. The inhibition of PKC and PI3K signaling (through NF-kappaB and AP-1) suppressed the secretion of uPA, resulting in the inhibition of motility of highly invasive breast cancer cells. Therefore, inhibition of specific target molecules in common signaling pathway(s) responsible for metastatic spread can have potential clinical relevance. This review will summarize different approaches to targeting distinct signaling molecules involved in cancer invasion and metastasis.