gebhart/patrepeval huggingface dataset:
A Compliant Hybrid Zero Dynamics Controller for Stable, Efficient and Fast Bipedal Walking on MABEL (2010)

The planar bipedal testbed MABEL contains springs in its drivetrain for the purpose of enhancing both energy efficiency and agility of dynamic locomotion. While the potential energetic benefits of springs are well documented in the literature, feedback control designs that effectively realize this potential are lacking. In this paper, we extend and apply the methods of virtual constraints and hybrid zero dynamics, originally developed for rigid robots with a single degree of underactuation, to MABEL, a bipedal walker with a novel compliant transmission and multiple degrees of underactuation. A time-invariant feedback controller is designed such that the closed-loop system respects the natural compliance of the open-loop system and realizes exponentially stable walking gaits. Five experiments are presented that highlight different aspects of MABEL and the feedback design method, ranging from basic elements such as stable walking and robustness under perturbations, to energy efficiency and a walking speed of 1.5 m s −1 (3.4 mph). The experiments also compare two feedback implementations of the virtual constraints, one based on PD control of Westervelt et al., and a second that implements a full hybrid zero dynamics controller. On MABEL, the full hybrid zero dynamics controller yields a much more faithful realization of the desired virtual constraints and was instrumental in achieving more rapid walking.
Definition and Validation of the Key process of Release, Delivery and Deployment for Product Software Vendors: turning the ugly duckling into a swan (2006)

For software vendors the processes of release, delivery, and deployment to customers are inherently complex. However, software vendors can greatly improve their product quality and quality of service by applying a model that focuses on customer interaction if such a model were available. This paper presents a model for customer configuration updating (CCU) that can evaluate the practices of a software vendor in these processes. Nine extensive case studies of medium to large product software vendors are presented and evaluated using the model, thereby uncovering issues in their release, delivery, and deployment processes. Finally, organisational and architectural changes are proposed to increase quality of service and product quality for software vendors
NDRG2 expression decreases with tumor stages and regulates TCF/ -catenin signaling in human colon carcinoma (2009)

NDRG (N-Myc downstream-regulated gene)-2 is a member of the NDRG family. Although it has been suggested that NDRG2 is involved in cellular differentiation and tumor suppression, its intracellular signal and regulatory mechanism are not well known. Here, we show the differential expression of NDRG2 in human colon carcinoma cell lines and tissues by reverse transcriptionpolymerase chain reaction and immunohistochemical analyses with monoclonal antibody against NDRG2. NDRG2 was strongly expressed in normal colonic mucosa and colonic adenomatous tissues (25 of 25) but not in all invasive cancer tissues [44 of 99 (44%)]. Most distinctive results indicated that the high expression level of NDRG2 has a positive correlation with tumor differentiation and inverse correlation with tumor invasion depth and Dukes' stage of colon adenocarcinoma. To investigate the roles of NDRG2 in tumorigenesis, we used in vitro cell culture system. SW620 colon cancer cell line with a low level of intrinsic NDRG2 protein was transfected with NDRG2-expressing plasmid. TOPflash luciferase reporter assay showed that the transcriptional activity of T-cell factor (TCF)/lymphoid enhancer factor (LEF) was reduced by NDRG2 introduction, but not by the introduction of mutant NDRG2 generated by deletion or sitedirected mutagenesis. Intracellular b-catenin levels were slightly reduced in the NDRG2-transfected SW620 cells and this regulation of b-catenin stability and TCF/LEF activity were mediated through the modulation of glycogen synthase kinase-3beta activity by NDRG2 function. Our results suggest that NDRG2 might play a pivotal role as a potent tumor suppressor by the attenuation of TCF/b-catenin signaling for the maintenance of healthy colon tissues.
Translational stability of native and deadenylylated rabbit globin mRNA injected into HeLa cells. (1981)

HeLa human cells were injected with a natural mixture of rabbit alpha and beta globin mRNA. They were incubated for 6 hr with [35S]methionine either immediately after injection or 20 hr later. The labeled proteins in the injected cells were analyzed by fluorography of two-dimensional electrophoresis gels. By using this procedure, it was possible to show that, during the first few hours after injection, both alpha and beta globin molecules are synthesized with an alpha to beta ratio approximately equal to 0.6. The rate of synthesis of alpha globin decreased significantly faster than that of beta globin over a 26-hr period after injection of the two mRNAs. It thus seems that two messenger RNAs coding for closely related polypeptides possess a markedly different translational stability. When deadenylylated rabbit globin mRNAs were injected into HeLa cells, no globin synthesis could be detected by the techniques used. We conclude that the translational half-life of mRNAs lacking poly(A) is very short in these cells. It is thus clear that the poly(A) segment is required to ensure stability to globin mRNA in somatic cells as in Xenopus oocytes.
Monitoring Routing Topology in Dynamic Wireless Sensor Network Systems (2015)

In large-scale multi-hop wireless sensor networks (WSNs) for data collection, the ability of monitoring per-packet routing paths at the sink is essential in better understanding network dynamics, and improving routing protocols, topology control, energy conservation, anomaly detection, and load balance in WSN deployments. In this study, we consider this important problem under tremendous WSN routing dynamics, which cannot be addressed by previous methods based on a routing tree model. We formulate the WSN topology inference as a novel optimization problem, and devise efficient decoding algorithms to effectively recover WSN routing topology at the sink in real-time using a small fixed-size path measurement attached to each packet. Rigorous complexity analysis of the devised algorithms is given. Performance evaluation is conducted via extensive simulations. The results reveal that our approach significantly outperforms other state-of-the-art methods including MNT, Pathfinder, and CSPR. Furthermore, we validate our approach intensively with a real-world outdoor WSN deployment running collection tree protocol for environmental data collection.
The Amino Terminus of Epstein-Barr Virus Glycoprotein gH Is Important for Fusion with Epithelial and B Cells (2005)

Epstein-Barr virus (EBV) infects B lymphocytes and epithelial cells. While the glycoproteins required for entry into these two cell types differ, the gH/gL glycoprotein complex is essential for entry into both epithelial and B cells. Analysis of gH protein sequences from three gammaherpesviruses (EBV, marmoset, and rhesus) revealed a potential coiled-coil domain in the N terminus. Four leucines located in this region in EBV gH were replaced by alanines by site-directed mutagenesis and analyzed for cell-cell membrane fusion with B cells and epithelial cells. Reduction in fusion activity was observed for mutants containing L65A and/or L69A mutations, while substitutions in L55 and L74 enhanced the fusion activity of the mutant gH/gL complexes with both cell types. All of the mutants displayed levels of cell surface expression similar to those of wild-type gH and interacted with gL and gp42. The observation that a conservative mutation of leucine to alanine in the N terminus of EBV gH results in fusion-defective mutant gH/gL complexes is striking and points to an important role for this region in EBV-mediated membrane fusion with B lymphocytes and epithelial cells.
Current Strategies in Immunotherapy for Acute Myeloid Leukemia (2012)

The prognosis of acute myeloid leukemia, particularly when associated with adverse chromosomal or molecular aberrations, is poor due to a high relapse rate after induction chemotherapy. Postremission therapy for elimination of minimal residual disease remains a major challenge. Allogeneic hematopoietic stem cell transplantation has proven to provide a potent antileukemic effect. Novel strategies are needed for patients ineligible for this treatment. Here current immunotherapeutic concepts in acute myeloid leukemia in a nonallogeneic hematopoietic stem cell transplantation setting are reviewed. Data gathered with different monoclonal antibodies are discussed. Adoptive transfer of NK and T cells is reviewed, including evolving data on T-cell engineering. Results of systemic cytokine administration and of therapeutic vaccinations with peptides, modified leukemic cells and dendritic cells are presented. One particular focus of this review is the integration of currently running clinical trials. Recent immunotherapeutic studies have been encouraging and further interesting results are to be expected.
Heat inactivation of the Severe Acute Respiratory Syndrome Coronavirus 2 (2020)

Abstract Supernatants of cells infected with SARS-CoV-2, nasopharyngeal and sera samples containing SARS-CoV-2 were submitted to heat inactivation for various periods of time, ranging from 30 seconds to 60 minutes. Our results showed that SARS-CoV-2 could be inactivated in less than 30 minutes, 15 minutes and 3 minutes at 56°C, 65°C and 95°C respectively. These data could help laboratory workers to improve their protocols with handling of the virus in biosafety conditions.
Sparse Unsupervised Capsules Generalize Better (2018)

We show that unsupervised training of latent capsule layers using only the reconstruction loss, without masking to select the correct output class, causes a loss of equivariances and other desirable capsule qualities. This implies that supervised capsules networks can't be very deep. Unsupervised sparsening of latent capsule layer activity both restores these qualities and appears to generalize better than supervised masking, while potentially enabling deeper capsules networks. We train a sparse, unsupervised capsules network of similar geometry to Sabour et al (2017) on MNIST, and then test classification accuracy on affNIST using an SVM layer. Accuracy is improved from benchmark 79% to 90%.
Photothermal model of plant development (2006)

The development of plants depends on the photoperiod length, light intensity, temperature, and length of light day integral. The reaction of a plant to the day length or daily light integral can depend on both the range of studied light intensities and photoperiod. Based on the data concerning the effects of light and thermal integrals on the developmental rate of plants of different photoperiodic groups, a photothermal model of plant development was proposed. The model was used to calculate the lengths of optimal photoperiods and ranges of daily temperature gradients ensuring the highest developmental rate of some plants, such as soybean, wheat, cucumber, and barley.
A high-efficiency 100-W four-stage Doherty GaN HEMT power amplifier module for WCDMA systems (2011)

In this paper, a novel high-efficiency four-stage Doherty power amplifier architecture convenient for practical implementation in base station applications for modern communication standards has been proposed and fabricated. In practical verification, each power amplifier is based on a 25-W Cree GaN HEMT device with the transmission-line load network corresponding to an inverse Class F mode approximation. In a CW operation mode with the same bias voltage for each transistor, an output power of 50 dBm with a drain efficiency of 77% was achieved at a supply voltage of 34 V. In a single-carrier WCDMA operation mode with PAR of 6.5 dB, a high drain efficiency of 61% was achieved at an average output power of 43 dBm, with ACLR <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> measured at -31 dBc level.
Optimization of N-carboxyanhydride (NCA) polymerization by variation of reaction temperature and pressure (2011)

Several new methods for the controlled ring-opening polymerization of N-carboxyanhydrides (NCA ROP) have been established in the recent past, all of which based on the normal amine mechanism. In this paper the optimal NAM polymerization conditions were investigated combining the high-vacuum and the low-temperature for the NCAs of γ-benzyl-L-glutamate (BLG), Nε-benzyloxycarbonyl-L-lysine (ZLL), L-alanine (Ala), β-benzyl-L-aspartate (BLA), O-benzyl-L-serine (BLS), and O-benzyl-L-threonine (BLT). The polymerizations were followed by FTIR, size exclusion chromatography (SEC) and MALDI-ToF-MS to provide information of the monomer conversion, polymer molecular weight and chain composition as a function of pressure and temperature. It was found that the studied NCAs could be divided into two groups: in the first group monomers of BLG, ZLL and Ala polymerized considerably faster when a lower pressure of 1 × 10−5 bar was applied. MALDI-ToF-MS analysis confirmed that the formation of side products for these monomers mostly started after full monomer conversion. The second group of monomers, i.e. BLA, BLS and BLT, polymerized considerably slower than the first group and no effect was observed from the lower pressure. On the other hand, the number of side reactions was significant at 20 °C, so that the polymerizations for the latter monomers should preferably be done at 0 °C. By combining both methods, multiblock polypeptides were synthesized including a tetrablock of PBLG-b-PAla-b-PZLL-b-PBLA with a polydispersity of 1.3.
Plastic Folding of Buckling Structures (2007)

Atomic force microscopy observations of the free surface of gold thin films deposited on silicon substrates have evidenced the buckling of the films and the formation of blister patterns undergoing plastic folding. The classical elastic buckling and plastic deformation of the films are analyzed in the framework of the Föppl-Von Kármán theory of thin plates introducing the notion of low-angle tilt boundaries and dislocation distributions to describe this folding effect. It is demonstrated that, in agreement with elementary plasticity of bent crystals, the presence of such tilt-boundaries results in the formation of buckling patterns of lower energy than "classical" elastic blisters.
Pump administration of exogenous surfactant: effects on oxygenation, heart rate, and chest wall movement of premature infants. (1993)

Adverse reactions of infants to surfactant administration include loss of chest wall movement and decrease in oxygen saturation and heart rate. We are aware of no previous studies addressing the administration of surfactant. We studied 22 infants with respiratory distress syndrome to determine whether these adverse reactions could be reduced by giving surfactant slowly by microinfusion syringe pump. Loss of chest wall movement was significantly greater in infants receiving surfactant by the hand-dosing method as compared with administration of surfactant by pump. A significant increase in ventilator pressure interventions was observed when surfactant was given by standard hand bolus method compared with pump infusion. Heart rate and oxygen saturation did not differ significantly between the two techniques. These findings suggest that pump administration of surfactant minimized potential airway obstruction; thus it may be an alternative to standard bolus dosing techniques.
Metabolism of Stevioside and Rebaudioside A from <i>Stevia rebaudiana</i> Extracts by Human Microflora (2003)

Stevia rebaudiana standardized extracts (SSEs) are used as natural sweeteners or dietary supplements in different countries for their content of stevioside or rebaudioside A. These compounds possess up to 250 times the sweetness intensity of sucrose, and they are noncaloric and noncariogenic sweeteners. The aim of this study was to investigate the in vitro transformation of stevioside and rebaudioside A after incubation with human microflora, the influence of these sweeteners on human microbial fecal community and which specific groups metabolize preferentially stevioside and rebaudioside A. The experiments were carried out under strict anaerobic conditions in batch cultures inoculated with mixed fecal bacteria from volunteers. The hydrolysis was monitored by HPLC coupled to photodiode array and mass spectrometric detectors. Isolated bacterial strains from fecal materials incubated in selective broths were added to stevioside and rebaudioside A. These sweeteners were completely hydrolyzed to their aglycon steviol in 10 and 24 h, respectively. Interestingly, the human intestinal microflora was not able to degrade steviol. Furthermore, stevioside and rebaudioside A did not significantly influence the composition of fecal cultures; among the selected intestinal groups, bacteroides were the most efficient in hydrolyzing Stevia sweeteners to steviol.
Current-induced guiding and beam steering in active semiconductor planar waveguide (1999)

We propose a novel method of achieving beam steering in active GaAs-AlGaAs materials. Carrier-induced refractive index change effect is applied to form a channel waveguide in a slab structure. Current injection is used as a means to control, guide, and steer an optical beam between two active stripes. In a structure that was 710 μm long, we have experimentally demonstrated waveguiding of an externally coupled optical beam and electrically induced steering of the beam over a 17-μm lateral distance at the output facet. The results of theoretical analysis and modeling of the beam shift fit the experimental data well.
Cloning of Cellulase Genes (1987)

Methodologie: strategies de clonage, identification et caracterisation des genes clones. Exemples: Cellulomonas fimi, Clostridium thermocellum, Thermomonospora fusca, Bacillus spp, Erwinia chrysanthemi, Bacteroides succinogenes, Pseudomonas spp, Trichoderma reesei. Perspectives
Ubiquitin Signaling: Extreme Conservation as a Source of Diversity (2014)

Around 2 × 103-2.5 × 103 million years ago, a unicellular organism with radically novel features, ancestor of all eukaryotes, dwelt the earth. This organism, commonly referred as the last eukaryotic common ancestor, contained in its proteome the same functionally capable ubiquitin molecule that all eukaryotic species contain today. The fact that ubiquitin protein has virtually not changed during all eukaryotic evolution contrasts with the high expansion of the ubiquitin system, constituted by hundreds of enzymes, ubiquitin-interacting proteins, protein complexes, and cofactors. Interestingly, the simplest genetic arrangement encoding a fully-equipped ubiquitin signaling system is constituted by five genes organized in an operon-like cluster, and is found in archaea. How did ubiquitin achieve the status of central element in eukaryotic physiology? We analyze here the features of the ubiquitin molecule and the network that it conforms, and propose notions to explain the complexity of the ubiquitin signaling system in eukaryotic cells.
Acceleration of Nucleophilic CH Activation by Strongly Basic Solvents (2010)

(IPI)Ru(II)(OH)(n)(H(2)O)(m), 2, where IPI is the NNN-pincer ligand, 2,6-diimidizoylpyridine, is shown to catalyze H/D exchange between hydrocarbons and strongly basic solvents at higher rates than in the case of the solvent alone. Significantly, catalysis by 2 is accelerated rather than inhibited by increasing solvent basicity. The evidence is consistent with the reaction proceeding by base modulated nucleophilic CH activation.
Effects of Nicotine on Human Luteal Cells In Vitro: A Possible Role on Reproductive Outcome for Smoking Women1 (2004)

We investigated the effect of nicotine and its methylated metabolite, N-methyl-nicotine (M-nicotine), on human luteal cells by measuring release of progesterone and prostaglandins (PGs) from cultured cells and by testing gene expression of vascular endothelial growth factor (VEGF), an angiogenic factor strictly involved in luteal pathophysiology. Primary cultures of human luteal cells were treated for 24 h with nicotine and M-nicotine (from 10(-6) to 10(-11) M) either alone or combined with hCG (25 ng/ml); progesterone and PGs were assayed in the culture medium. In another group of experiments, luteal cells were treated for 24 h with nicotine and M-nicotine (10(-7) M) to perform reverse transcriptase-polymerase chain reaction on VEGF mRNA. Nicotine and M-nicotine negatively affected basal luteal steroidogenesis at all tested concentrations, but neither was able to affect hCG-induced progesterone release. Both substances were able to significantly increase PGF2alpha release from luteal cells, with a dose-related efficacy for M-nicotine. On the contrary, PGE2 release was significantly inhibited by both nicotine and its metabolite. Finally, nicotine was able to increase VEGF mRNA expression significantly, whereas M-nicotine was not. In conclusion, nicotine and M-nicotine can induce a sort of luteal insufficiency by inhibiting progesterone release, probably through modulation of the PG system.
Polymer Electrolyte Gating of Carbon Nanotube Network Transistors (2005)

Network behavior in single-walled carbon nanotubes (SWNTs) is examined by polymer electrolyte gating. High gate efficiencies, low voltage operation, and the absence of hysteresis in polymer electrolyte gating lead to a convenient and effective method of analyzing transport in SWNT networks. Furthermore, the ability to control carrier type with chemical groups of the host polymer allows us to examine both electron and hole conduction. Comparison to back gate measurements is made on channel length scaling. Frequency measurements are also made giving an upper limit of approximately 300 Hz switching speed for poly(ethylene oxide)/LiClO(4) gated SWNT thin film transistors.
Radiographic evaluation of the wrist: what does the hand surgeon want to know? (1992)

Congenital, developmental, and acquired wrist deformities predispose patients to characteristic conditions and their associated debilities. The accurate recognition and quantitation of these conditions represent guideposts to treatment and prognosis. The authors present mensuration methods and normal ranges for the important morphologic features of carpal height, ulnar variance, radial inclination, radial length, palmar tilt, and radial shift. These measurements assume importance in the description and quantitation of many conditions. The authors review the radiographic features and diagnosis of dissociated and nondissociated carpal instability, scapholunate advanced collapse, ulnar translocation, ulnocarpal impaction, and ulnoradial impingement.
Unit delay simulation with the inversion algorithm (1996)

The Inversion Algorithm is an event driven algorithm whose performance meets or exceeds that of Levelized Compiled Code simulation, even when the activity rate is unrealistically high. Existing implementations of the Inversion Algorithm are based on the Zero Delay model. This paper extends the algorithm to more realistic timing models. The main problems discussed in this paper are avoiding scheduling conflicts, and minimizing the amount of storage space. These problems are made considerably more difficult by the deletion of NOT gates and the collapsing of various connections. These optimizations transform the simulation into a multi-delay simulation under the transport delay model. A complete solution to the scheduling problem is presented under these conditions.