gebhart/patrepeval huggingface dataset:
Theory and basic sciences (1996)

Physical Characterization of Liposomes for Understanding Structure-Function Relationships in Biological Membranes, D. Marsh The Suitability of Liposomes as Models for Studying Lipid Phase Transitions and Lipid Organization and Dynamics in Biological Membranes, R.N. McElhaney Consequences of Phase Separations in Membranes, W.L.C. Vaz What Do Probe Molecules Monitor in Lipid Bilayers?, U.A. van der Heide, H. Eviatar, and Y.K. Levine Characterization of Liposomes and Vesicles by 31P-NMR, P.L. Yeagle LIPIDAT: A Database of Lipid Phase Transition Temperatures, Enthalpy Changes, and Associated Information, M. Caffrey, R. Koynova, J. Hogan, and D. Moynihan Liposomes and Membrane Stability, R.M. Epand and I. Polozov Membrane Systems as Models for Studying Permeabilization Exemplified by Polyene Antibiotics, C. Salerno, C. Crif'o, and E. Capuozzo Liposomes as a Tool to Study Blood Coagulation, B.R. Lentz Use of Liposomes for Administering Fluorescent Lipids and Studying Their Metabolism in Normal and Lipidotic Cells, S. Gatt, T. Dinur, S. Cerbu-Karabat, A. Dagan, and V. Agmon Vesicles in Biological Systems and the Origin of Life-A Foreword to Section Two, Y. Barenholz and D. Lasic Specifically Designed Lipid Assemblies as Tools for Two-Dimensional Crystallization of Soluble Biological Macromolecules, L. Lebeau, P. Schultz, H. Celia, P. Mesini, S. Nuss, C. Klinger, S. Olland, P. Oudet, and C. Mioskowski Supermolecular Assembly of Peptide Molecules in Liposomes, Y. Imanishi and S. Kimura Liposomes as a Model for Solubilization and Reconstitution of Membranes, D. Lichtenberg Liposomes in Reconstitution of Ion-Pumps, F. Cornelius and J.V. Moller Liposomes in Reconstitution of Proton-Motive Proteins, S. Papa, M. Lorusso, N. Capitanio, and F. Zanotti Reconstitution of Gap Junction Channels: Liposomes as Tools to Assay the Activity of Channel-Forming Proteins, J.A. Diez and A. Villalobo Kinetic, Thermodynamic, and Spectroscopic Studies of the Interactions between Redox Proteins and Lipid Bilayers, G. Tollin, Z. Salamon, and G. Cheddar Transbilayer Transport Induced by Transmembrane pH Gradients in Liposomes: Implications for Biological Systems, M.J. Parr and P.R. Cullis Liposome Models of Primitive Membrane Structures, D.W. Deamer and A.C. Chakrabarti Self-Reproducing Liposomes and Chemicals Autopoiesis, P.L. Luigi and P. Walde Lipid Vesicles as Model Systems for Archaea Membranes, A. Gliozzi and A. Relini Liposomes in Biomineralization, E.D. Eanes Liposome Interaction with Hydroxyapatite May Explain Calcification in Biological Systems, S. Sarig Index
C-Terminal Cyclization of an SDF-1 Small Peptide Analogue Dramatically Increases Receptor Affinity and Activation of the CXCR4 Receptor (2002)

In an effort to improve the activities and bioavailabilities of stromal cell-derived factor-1 (SDF-1, CXCL12) sdf-(1-67)-OH (1), we have prepared a linear peptide analogue [sdf-(1-31)-NH(2) (2)] and two lactam analogues [cyclo(Lys(20)-Glu(24))-sdf-(1-31)-NH(2) (3) and cyclo(Glu(24)-Lys(28))-sdf-(1-31)-NH(2) (4)], consisting of the N-terminal region (amino acids 1-14) joined by a four-glycine linker to the C-terminal region (amino acids 56-67) of 1. Analogues 2 and 4 had eight residues of alpha-helix, as estimated from its circular dichroism (CD) spectra, in contrast to 10 residues in analogue 3. Cyclization of analogue 2 at residues 20 and 24 to give analogue 3 resulted in only a slight change to the theta;(222)/theta;(209) ratio (0.81 to 0.86, where 1.09 is considered a perfect alpha-helix), although an increase in the alpha-helix length of analogue 3 was observed. In contrast, cyclization between residues 24 and 28 by lactamization to give analogue 4 only slightly affected the helical content but clearly resulted in a more classical alpha-helical structure (theta;(222)/theta;(209) = 0.98). Cyclization of the linear analogue 2 enhanced the SDF-1 receptor CXCR4 binding approximately 114-fold, where the IC(50) values derived from (125)I-SDF-1 competitive binding assays with CEM cells were found to be 39.5 +/- 5.9 nM, 28.9 +/- 6.3 microM, 225.8 +/- 11.8 nM, and 254.1 +/- 5.4 nM for analogues 1-4, respectively. Intracellular calcium mobilization ([Ca(2+)](i)) induced after interaction with CXCR4, as measured by EC(50), was significantly reduced in analogue 4 compared to 3, and approached the EC(50) of native SDF-1, indicating a correlation between the degree of alpha-helix and biological activity. Therefore, the biological activity of small peptide SDF-1 analogues is highly dependent on the conformation of its C-terminal region.
Strategies for manufacturing recombinant adeno-associated virus vectors for gene therapy applications exploiting baculovirus technology (2008)

The development of recombinant adeno-associated virus (rAAV) gene therapy applications is hampered by the inability to produce rAAV in sufficient quantities to support pre-clinical and clinical trials. Contrasting with adherent cell cultures, suspension cultures provide a straightforward means for expansion, however, transiently expressing the necessary, but cytotoxic virus proteins remains the challenge for rAAV production. Both the expansion and expression issues are resolved by using the baculovirus expression vector (bev) and insect cell culture system. This review addresses strategies for the production of rAAV exploiting baculovirus technology at different scales using different configurations of bioreactors as well as processing and product characterization issues. The yields obtained with these optimized processes exceed approximately 1 x 10(14) vector particles per liter of cell culture suitable for pre-clinical and clinical trials and possible commercialization.
Increased Iron and Free Radical Generation in Preclinical Alzheimer Disease and Mild Cognitive Impairment (2010)

It is now established that oxidative stress is one of the earliest, if not the earliest, change that occurs in the pathogenesis of Alzheimer's disease (AD). Consistent with this, mild cognitive impairment (MCI), the clinical precursor of AD, is also characterized by elevations in oxidative stress. Since such stress does not operate in vacuo, in this study we sought to determine whether redox-active iron, a potent source of free radicals, was elevated in MCI and preclinical AD as compared to cognitively-intact age-matched control patients. Increased iron was found at the highest levels both in the cortex and cerebellum from the pre-clinical AD/MCI cases. Interestingly, glial accumulations of redox-active iron in the cerebellum were also evident in preclinical AD patients and tended to increase as patients became progressively cognitively impaired. Our findings suggests that an imbalance in iron homeostasis is a precursor to the neurodegenerative processes leading to AD and that iron imbalance is not necessarily unique to affected regions. In fact, an understanding of iron deposition in other regions of the brain may provide insights into neuroprotective strategies. Iron deposition at the preclinical stage of AD may be useful as a diagnostic tool, using iron imaging methods, as well as a potential therapeutic target, through metal ion chelators.
FOCAL: Forwarding and Caching with Latency Awareness in Information-Centric Networking (2015)

Latency minimization is an important network optimization criterion which becomes an even more compelling feature in 5G networks. Information-Centric Networking (ICN) appears a promising candidate technology for building an agile communication model that reduces latency via a fully distributed and adaptive delivery approach coupling in-network caching and forwarding. In the paper, we investigate the role of latency awareness on ICN delivery performance and introduce FOCAL, an approach combining novel caching and forwarding strategies to jointly reduce end-user experienced latency with no network signaling nor coordination between routers. FOCAL gathers a latency-proportional probabilistic caching policy, with a load-aware dynamic forwarding strategy, that preferentially routes popular content requests through a single path (set of caches), while globally achieving minimum network load and user content delivery time, thus delay minimization. By means of ICN simulations, we assess the superiority of FOCAL over existing alternatives given by the combinations of known caching policies and forwarding strategies: It results a reduced end-user delivery performance coupled with faster convergence to average/variance figure and higher self-adaptiveness to varying traffic/network conditions.
Synthesis and rheological properties of soluble poly(hydroxyethyl methacrylate) and some copolymers (1995)

Abstract Methods to synthesise soluble poly(2‐hydroxyethyl methacrylate) (PHEMA) with varying molar mass were developed. Conversions over 90% were achieved without losing solubility. Steady state tests (range 0.01–590 Pa) and oscillatory tests (range 0.001–40 Hz) were carried out on 40% solutions in dimethyl formamide using a Carri‐Med rheometer. Only PHEMA having molar mass over 500000 showed significant shear thinning and viscoelastic properties. The copolymers of 2‐hydroxyethyl methacrylate (HEMA) with n ‐butyl acrylate (BA) and 2‐hydroxypropyl acrylate (HPA) showed higher viscosities and viscoelastic properties compared with PHEMA homopolymer prepared under identical conditions. Also, viscosity and viscoelasticity increased with increase in acrylate content in the initial mixture. This was attributed to the higher molar mass of copolymers, which resulted from faster polymerisation rates owing to the inclusion of relatively highly reactive acrylates. However, in the case of HEMA : BA copolymers, first, the viscosity and viscoelasticity increased with increasing BA content and then dropped again, giving a maximum around HEMA : BA 75 : 25. This anomaly was explained by taking the effects of changes in inter‐and intra‐molecular hydrogen bonding as well as conformational differences caused by inclusion of BA in the PHEMA chain into account.
Cellulose Nanomaterials in Water Treatment Technologies (2015)

Cellulose nanomaterials are naturally occurring with unique structural, mechanical and optical properties. While the paper and packaging, automotive, personal care, construction, and textiles industries have recognized cellulose nanomaterials' potential, we suggest cellulose nanomaterials have great untapped potential in water treatment technologies. In this review, we gather evidence of cellulose nanomaterials' beneficial role in environmental remediation and membranes for water filtration, including their high surface area-to-volume ratio, low environmental impact, high strength, functionalizability, and sustainability. We make direct comparison between cellulose nanomaterials and carbon nanotubes (CNTs) in terms of physical and chemical properties, production costs, use and disposal in order to show the potential of cellulose nanomaterials as a sustainable replacement for CNTs in water treatment technologies. Finally, we comment on the need for improved communication and collaboration across the myriad industries invested in cellulose nanomaterials production and development to achieve an efficient means to commercialization.
Personal communications network evolution (1996)

The introduction of personal communications networks (PCNs) coincided with the change in mobile cellular technology from analog to digital. However the rapid growth and take up of personal communication services has been affected as much by regulatory and marketing changes as the introduction of second generation technology. A key characteristic foreseen for the next generation is that of bringing together all attributes of a personal communications service into a single unified system. In the short term, multi-mode terminals will link networks based on cordless, cellular, satellite and fixed network technologies. A single number and single bill for all telecommunications services will become common. As IN techniques are used more fully, least cost routing and consistent service features will be achieved. The proposed third generation standards, the Future Public Land Mobile Telecommunications System (FPLMTS), and the Universal Mobile Telecommunication System (UMTS) will extend the range of services including those utilising higher speed data, such that new radio air interfaces and access methods will be required. However, due to the investment in existing networks the universal personal communications services of the future will be provided by a combination of evolved second generation networks with enhanced features and functions of third generation systems. At the service level, these mobile systems will provide the primary access to the `Information Society' in which the availability of information, anytime, anywhere will revolutionise lifestyles. (9 pages)
Extremely simple nonlinear noise-reduction method (1993)

A very simple method to reduce noise in experimental data with nonlinear time evolution is presented. Locally constant fits are used to obtain a less noisy trajectory consistent with the dynamics as well as with the measured data. Neighborhoods are defined by coordinates both from the past and from the future. The method is applied to the H\'enon map and to a discretized form of the Mackey-Glass equation.
Straightforward Synthesis of Metal Nanoparticles and Hierarchical Porous Metals Assisted by Partial Film Boiling Phenomena (2015)

The development of an eco-friendly and economical synthetic pathway of nanomaterials such as metal nanoparticles and nanoporous metals remains a challenging topic in the field of nanomaterials. Here we report a novel and eco-friendly series of synthetic methods from the preparation of metal nanoparticles to the fabrication of hierarchical porous metals by using partial film boiling phenomena established by a reactor with the inverse configuration of conventional Leidenfrost drop reactors, which is much more favorable to large-scale production than conventional Leidenfrost drop reactors. As a result, we have revealed two important facts, which can offer fresh vision to the field of metal nanoparticle and nanoporous metal synthesis. The first one is that Pd and Pt precursor can be reduced to neutral Pd and Pt nanoparticles under basic condition established by partial film boiling phenomena without reducing agents such as ethanol and citric acid. The second one is that when citric acid and ethanol as a mild reducing agent were added into the precursor aqueous solution, citric acid also plays an important role as a pore-forming agent, and ethanol facilitates the aggregation of as-prepared metal nanoparticles, resulting in the formation of nanoporous Pd and Pt. Our work is believed to suggest more eco-friendly and straightforward approaches for fabrication of metal nanoparticles and nanoporous metals. However, further research is required to explore whether our novel synthetic pathway is available to other metal systems.
Hubungan Mutu Pelayanan Perawat dengan Kepuasan Pasien di Ruang Rawat Inap Rumah Sakit Syafira Pekabaru Tahun 2018 (2019)

Kepuasan pasien di ruang rawat inap sangatlah penting untuk diperhatikan sebab secara tidak langsung menjadi faktor pendorong bagi kesehatan pasien dengan cara meningkatkan mutu pelayanan seperti kehandalan, jaminan, bukti fisik, empati, dan daya tanggap. Tujuan penelitian ini untuk mengetahui hubungan Reliability (Reliabilitas/Kehandalan), Assurance (Jaminan), Tangibles (Tampilan/Bukti fisik), Emphaty (Empati), dan Responsiveness (Ketanggapan dan kepedulian) dengan Kepuasan Pasien. Jenis penelitian ini adalah kuantitatif dengan desain cross sectional. Sampel dalam penelitian ini adalah 99 pasien rawat inap di Rumah Sakit Syafira Pekanbaru dengan menggunakan teknik simple random sampling. Pengumpulan data dilakukan dengan menggunakan kuesioner dan observasi. Analisis yang digunakan adalah analisis univariat dan bivariat dengan uji statistik Chi-Square (p =0,05). Hasil penelitian menunjukkan bahwa ada hubungan antara Kehandalan (p value = 0,001 dan nilai OR 79,625), Jaminan (p value = 0,001 dan nilai OR 142,857), Bukti Fisik (p value = 0,001 dan nilai OR 6,578), Empati (p value = 0,001 dan nilai OR 93,333), Daya Tanggap (p value = 0,001 dan nilai OR 170,833) dengan Kepuasan Pasien. Saran bagi Perawat sebaiknya dapat memberikan pelayanan yang prima dengan niat yang tulus, sehingga memberikan kesan yang positif terhadap mutu pelayanan keperawatan yang dapat meningkatkan kepuasan pasien. Saran bagi Rumah Sakit Syafira Pekanbaru diharapkan kepada pihak Manajemen Rumah Sakit Syafira Pekanbaru untuk lebih meningkatkan mutu pelayanan keperawatan guna memenuhi kepuasan pasien.
Bacillus vallismortis EXTN-1-Mediated Growth Promotion and Disease Suppression in Rice (2006)

Bacillus vallismortis EXTN-1, a biocontrol agent in cucumber, tomato and potato was tested in rice pathosystem against rice fungal pathogens viz. Magnaporthe grisea, Rhizoctonia solani and Cochliobolus miyabeanus. Apart from increasing the yield in the bacterized plants (11.6-12.6% over control), the study showed that EXTN1 is effective in bringing about disease suppression against all the tested fungal pathogens. EXTN-l treatment resulted in 52.11% reduction in rice blast, 83.02% reduction in sheath blight and 11.54% decrease in brown spot symptoms. As the strain is proven as an inducer for systemic resistance based on PR gene expression in Arabidopsis and tobacco models, it is supposed that a similar mechanism works in rice, bringing about disease suppression. The strain could be used as a potent biocontrol and growth-promoting agent in rice cropping system.
Coiled Tubing Conveyed Fracture Treatments: Evolution, Methodology and Field Application (1999)

Abstract A common limitation of fracture stimulation is the inability to control placement of a hydraulic fracture. This shortcoming compromises the effectiveness of a fracture treatment for multistage treatments during early field development, as well as single stage fracture stimulation of bypassed zones in mature reservoirs. This limitation can be overcome by integrating coiled tubing with hydraulic fracturing. This combination adds versatility to the fracturing procedure. It also permits control of fracture placement and hence can be used to improve the economic benefits associated with hydraulic fracturing. The effectiveness of coiled tubing fracturing operations can be constrained by excessive friction pressures, equipment limitations and limited fluid and proppant volumes. Its application thus requires specialized fracture fluids, additional field operational considerations, and alternative design procedures to ensure an efficient and successful fracture stimulation program. The paper initially explains the motivation for coiled tubing fracture treatments. Operational and design requirements associated with this technique are then outlined. Two case studies are finally presented to illustrate the application of the coiled tubing fracturing procedure.
Intrinsic binding of 4‐substituted‐2,3,5,6‐tetrafluorobenezenesulfonamides to native and recombinant human carbonic anhydrase VI (2015)

Carbonic anhydrase (CA) VI is a potential drug target for cariogenesis and cancer of the salivary gland. It is the only secreted human CA isozyme which is found in saliva and milk. Here, CA VI was expressed in bacterial and mammalian cell cultures and directly affinity-purified from human saliva. The binding of 4-substituted-2,3,5,6-tetrafluorobenezenesulfonamides to the native and recombinant CA VI from these three sources was compared. Interaction between the enzyme and inhibitors was determined by fluorescent thermal shift assay and isothermal titration calorimetry. The observed dissociation constants were the same within the error margin for all three CA VI preparations. The intrinsic binding parameters for the compounds were obtained by determining and dissecting the binding-linked protonation reactions. Intrinsic thermodynamic parameters of binding arrange the compounds in a buffer- and pH-independent manner. Intrinsic binding constants of nonfluorinated compounds were significantly stronger than those of fluorinated benzenesulfonamides. An opposite result was determined for the observed binding constants. The increase in observed affinity of the fluorinated compounds was due to the fluorine effect on diminishing the pKa of the compounds but not due to direct recognition of the protein. The temperature-stability profiles for recombinant and native CA VI were compared and showed that CA VI is more stable in slightly acidic than neutral conditions.
Energy-Efficient Cooperative Opportunistic Positioning for Heterogeneous Mobile Devices (2012)

The fast growing popularity of smartphones and tablets enables us the use of various intelligent mobile applications. As many of those applications require position information, a smart mobile device provides positioning methods such as GPS, WiFi, or Cell-ID based positioning services. However, those positioning methods have different characteristics of energy efficiency, accuracy, and service availability. In this paper, we present an Energy- Efficient Cooperative and Opportunistic Positioning System (ECOPS) for heterogeneous mobile devices. ECOPS facilitates mobile devices with estimated locations using WiFi in cooperation with a few available GPS broadcasting devices, in order to achieve high energy efficiency and accuracy within available budget constraints. ECOPS estimates the location using heterogeneous positioning services and the combination methods including a received signal strength indicator, 2D trilateration, and available power measurement of mobile devices. The evaluation shows that ECOPS significantly reduces energy consumption and achieves the good accuracy of a location.
RDCL 3D, a model agnostic web framework for the design and composition of NFV services (2017)

We present RDCL 3D, a “model agnostic” web framework for the design and composition of NFV services and components. The framework allows editing and validating the descriptors of services and components both textually and graphically and supports the interaction with external orchestrators or with deployment and execution environments. RDCL 3D is open source and designed with a modular approach, allowing developers to “plug in” the support for new models. We describe several advances with respect to the NFV state of the art, which have been implemented with RDCL 3D. We have integrated in the platform the latest ETSI NFV ISG model specifications for which no parsers/validators were available. We have also included in the platform the support for OASIS TOSCA models, reusing existing parsers. Then we have considered the modelling of components in a modular software router (Click), which goes beyond the traditional scope of NFV. We have further developed this approach by combining traditional NFV components (Virtual Network Functions) and Click elements in a single model. Finally, we have considered the support of this solution using the Unikernels virtualization technology.