gebhart/patrepeval huggingface dataset:
Key Role of the <i>Cdx2</i> Homeobox Gene in Extracellular Matrix–mediated Intestinal Cell Differentiation (1997)

To explore the role of homeobox genes in the intestine, the human colon adenocarcinoma cell line Caco2-TC7 has been stably transfected with plasmids synthesizing Cdx1 and Cdx2 sense and antisense RNAs. Cdx1 overexpression or inhibition by antisense RNA does not markedly modify the cell differentiation markers analyzed in this study. In contrast, Cdx2 overexpression stimulates two typical markers of enterocytic differentiation: sucrase-isomaltase and lactase. Cells in which the endogenous expression of Cdx2 is reduced by antisense RNA attach poorly to the substratum. Conversely, Cdx2 overexpression modifies the expression of molecules involved in cell-cell and cell-substratum interactions and in transduction process: indeed, E-cadherin, integrin-beta4 subunit, laminin-gamma2 chain, hemidesmosomal protein, APC, and alpha-actinin are upregulated. Interestingly, most of these molecules are preferentially expressed in vivo in the differentiated villi enterocytes rather than in crypt cells. Cdx2 overexpression also results in the stimulation of HoxA-9 mRNA expression, an homeobox gene selectively expressed in the colon. In contrast, Cdx2-overexpressing cells display a decline of Cdx1 mRNA, which is mostly found in vivo in crypt cells. When implanted in nude mice, Cdx2-overexpressing cells produce larger tumors than control cells, and form glandular and villus-like structures. Laminin-1 is known to stimulate intestinal cell differentiation in vitro. In the present study, we demonstrate that the differentiating effect of laminin-1 coatings on Caco2-TC7 cells is accompanied by an upregulation of Cdx2. To further document this observation, we analyzed a series of Caco2 clones in which the production of laminin-alpha1 chain is differentially inhibited by antisense RNA. We found a positive correlation between the level of Cdx2 expression, that of endogenous laminin-alpha1 chain mRNA and that of sucrase-isomaltase expression in these cell lines. Taken together, these results suggest (a) that Cdx1 and Cdx2 homeobox genes play distinct roles in the intestinal epithelium, (b) that Cdx2 provokes pleiotropic effects triggering cells towards the phenotype of differentiated villus enterocytes, and (c) that Cdx2 expression is modulated by basement membrane components. Hence, we conclude that Cdx2 plays a key role in the extracellular matrix-mediated intestinal cell differentiation.
A direct digital synthesis chirped pulse Fourier transform microwave spectrometer (2013)

Chirped pulse Fourier transform microwave (CP-FTMW) spectrometers have become the instrument of choice for acquiring rotational spectra, due to their high sensitivity, fast acquisition rate, and large bandwidth. Here we present the design and capabilities of a recently constructed CP-FTMW spectrometer using direct digital synthesis (DDS) as a new method for chirped pulse generation, through both a suite of extensive microwave characterizations and deep averaging of the 10-14 GHz spectrum of jet-cooled acetone. The use of DDS is more suited for in situ applications of CP-FTMW spectroscopy, as it reduces the size, weight, and power consumption of the chirp generation segment of the spectrometer all by more than an order of magnitude, while matching the performance of traditional designs. The performance of the instrument was further improved by the use of a high speed digitizer with dedicated signal averaging electronics, which facilitates a data acquisition rate of 2.1 kHz.
The Effect of Binder Chemistry on the Fracture Toughness of Cemented Tungsten Carbides (1984)

The fracture toughness of WC-10Co compositions with varying carbon concentrations were investigated. Both the material property, KIcSR and the apparent toughness, KQ, decreased rapidly upon the appearance of eta carbides in the microstructure. The amount of tungsten in the cobalt binder, as well as the presence of free carbon or eta carbides in the microstructure, affected the magnitude of the macroscopic residual stresses in the cermet. The fracture toughness of compositions in which varying amounts of nickel had been substituted for cobalt were also investigated. It was found that up to 25% nickel could be substituted for cobalt without adversely affecting the fracture toughness or the hardness of the cermet.
Embark: securely outsourcing middleboxes to the cloud (2016)

It is increasingly common for enterprises and other organizations to outsource network processing to the cloud. For example, enterprises may outsource firewalling, caching, and deep packet inspection, just as they outsource compute and storage. However, this poses a threat to enterprise confidentiality because the cloud provider gains access to the organization's traffic.

We design and build Embark, the first system that enables a cloud provider to support middlebox outsourcing while maintaining the client's confidentiality. Embark encrypts the traffic that reaches the cloud and enables the cloud to process the encrypted traffic without decrypting it. Embark supports a wide-range of middleboxes such as firewalls, NATs, web proxies, load balancers, and data exfiltration systems. Our evaluation shows that Embark supports these applications with competitive performance.
A High-Order Analysis of the Distortion Effects of Nonlinear Amplifiers on CDMA Signals (2008)

This paper presents a direct method of analyzing the distortion effects of the power amplifier nonlinearity on the CDMA signal. In the analysis approach of the paper, the various user signals, which are composed of data-modulated Walsh codes, which are also modulated by various scrambling codes, are modeled as independent random sequences. These sequences are band limited, using square-root raised cosine filters and modulated by a carrier frequency before being input to the nonlinear power amplifier. In the analysis approach of the paper, the amplifier characteristics are approximated by a polynomial of sufficiently high degree such that over the range of input signal, the approximation error is negligible. For the region of amplifier operation of interest in this paper, terms of degree higher than forty may be needed compared to an earlier simplified analysis presented by the author wherein only cubic distortion terms have been considered. The expressions for both the output signal power and the output signal power-to-distortion power spectral density ratio are given in the form of a finite series with its coefficients being a function of the amplifier input power level, the number of codes, and the amplifier transfer characteristics. The paper also shows that the amplifier possesses a maximum capacity defined as the maximum total bit rate Rt that can be present at the amplifier input for a specified output signal power back-off and the required output bit energy to distortion power spectral density ratio (E <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">b</sub> /N <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0d</sub> ). Also for any given output back-off and user data rate, the (E <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">b</sub> /N <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0d</sub> ) ratio is a strong function of the number of codes M in the CDMA signal.
Gene amplification and expression by RNA viruses and potential for further application to plant gene transfer (1990)

The great majority of plant viruses encapsidate messenger‐sense ssRNA and have no natural DNA phase in their life cycle. Despite their RNA nature, essentially any desired change can be introduced into such genomes by using recombinant DNA techniques with suitably constructed, expressible viral cDNA clones. For some viruses such as brome mosaic virus, these methods have been used to define the sequences controlling RNA‐directed genomic RNA replication and the expression of internal genes via subgenomic mRNAs. The results suggest a surprising degree of genetic flexibility, which appears to be reflected in the varied gene complements and genetic organizations of presumably related plant and animal RNA viruses sharing conserved replication genes. Foreign genes inserted in such RNA virus genomes can be amplified and expressed to a high level in transfected plant cells. In addition to the potential use of such viruses as episomal expression vectors, it should be possible to couple the viral pathways of RNA‐dependent RNA synthesis to amplify and to further regulate the expression of genes transformed into plant chromosomes.
Characteristics of Pulse-Reverse Electrodeposited Cu Thin Film (2012)

Pulse-reverse electrodeposition is capable of improving the superfilling ability and other properties of Cu in device interconnections. In this research, the influences of pulse-reverse electrodeposition on the properties of Cu thin film were investigated in the presence of organic additives used for Cu superfilling. The anodic step of pulse-reverse electrodeposition gave rise to changes in crystallinity, resistivity, grain size, and surface roughness. Minimum resistivity at the optimum dissolution ratio was observed, revealing a 14% reduction of the resistivity as compared to pulse electrodeposition samples. This was ascribed to the minimization of surface roughness produced by the selective dissolution of SPS-covered Cu as opposed to PEG-Cl−-covered Cu. It was confirmed that the selectivity of the anodic step came from the adsorbates and the anodic potential, resulting in changes in the film properties.
Purification and properties of a vanadate- and N-ethylmaleimide-sensitive ATPase from chromaffin granule membranes. (1988)

A vanadate- and N-ethylmaleimide-sensitive ATPase was purified about 500-fold from chromaffin granule membranes. The purified preparation contained a single major polypeptide with an apparent molecular mass of about 115 kDa, which was copurified with the ATPase activity. Immunological studies revealed that this polypeptide has no relation to subunit I (115 kDa) of the H+-ATPase from chromaffin granules. The ATPase activity of the enzyme is inhibited about 50% by 100 microM N-ethylmaleimide or 5 microM vanadate. The enzyme is not sensitive to dicyclohexylcarbodiimide, ouabain, SCH28080, and omeprazole, which distinguishes it from Na+/K+-ATPase and the gastric K+/H+-ATPase. ATP and 2-deoxy ATP are equally effective substrates for the enzyme. However, the enzyme exhibited only 10% activity with GTP as a substrate. UV illumination of the purified enzyme in the presence of [alpha-32P]ATP exclusively labeled the 115 kDa protein. This labeling was increased by Mg2+ and strongly inhibited by Ca2+ ions. Similarly, the ATPase activity was dependent on Mg2+ and inhibited by the presence of Ca2+ ions. The ATPase activity of the enzyme was largely insensitive to monovalent anions and cations, except for F-, which inhibited the vanadate-sensitive ATPase. Incubation of the enzyme in the presence of [14C]N-ethylmaleimide labeled the 115-kDa polypeptide, and this labeling could be prevented by the addition of ATP during the incubation. A reciprocal experiment showed that preincubation with N-ethylmaleimide inhibited the labeling of the 115-kDa polypeptide by [alpha-32P]ATP by UV illumination. This suggests a close proximity between the ATP-binding site and an essential sulfhydryl group. A possible connection between the isolated ATPase and organelle movement is discussed.
Radiofrequency-Controlled Catheter Pacemaker (1966)

THE transvenous endocardial electrode catheter1 and the radiofrequency induction assembly2 have been used for cardiac pacing with success over the past seven years. The catheter electrode has proved to be a satisfactory tool for short-term and long-term pacing and has been particularly useful for gaining immediate control of, and preserving, the heartbeat until installation of a more permanent unit.3 , 4 Of the difficulties encountered with its early application, only the transmission of infection via the exteriorized electrodes and perforation of the heart continue to be potentially serious. A recent report describes a method of long-term pacing with the pacemaker buried beneath . . .
Phase and Amplitude Stability of EHF-Band Radar Carriers Generated From an Active Mode-Locked Laser (2011)

We study the phase and amplitude stability of RF signals, generated at different frequencies by heterodyning pairs of modes from the optical spectrum of an active mode-locked laser, and we focus on the dependence of noise on the RF frequency. A specific theoretical model is derived, and the amplitude and timing jitter behaviour of the RF signals are analyzed and experimentally validated. The timing jitter reveals to be constant at any RF generated frequency, making the considered RF generation method suitable for the realization of flexible and highly stable micro- and millimeter-wave coherent radar transceivers. The performance of the considered RF generation architecture are compared with a state-of-the-art RF synthesizer, proving that the optically generated RF signals meet the high stability requirements of the new generation of coherent radar systems, even at extremely high frequencies (EHF).
Construction of a Positron Emission Tomograph with 2.4 Mm Detectors (1986)

One-quarter of one ring of a positron tomograph has been constructed. The positron annihilation gamma rays are detected by polished plastic scintillators which direct scintillation light by internal reflection to optical fibers for transmission to the photomultiplier tubes. By viewing each scintillator with four sets of optical fibers, the number of photomultipliers required for an eight ring tomograph with 1024 detectors per ring (2.4 mm wide detectors) can be reduced from 8192 to 288, and the cost of the tomograph reduced accordingly.
Query optimization in distributed networks of autonomous database systems (2006)

Large-scale distributed environments, where each node is completely autonomous and offers services to its peers through external communication, pose significant challenges to query processing and optimization. Autonomy is the main source of the problem, as it results in lack of knowledge about any particular node with respect to the information it can produce and its characteristics, for example, cost of production or quality of produced results. In this article, inspired by e-commerce technology, we recognize queries as commodities and model query optimization as a trading negotiation process. Subquery answers and subquery operator execution jobs are traded between nodes until deals are struck with some nodes for all of them. Such trading may also occur recursively, in the sense that some nodes may play the role of intermediaries between other nodes (subcontracting). We identify the key parameters of the overall framework and suggest several potential alternatives for each one. In comparison to trading negotiations for e-commerce, query optimization faces unique new challenges that stem primarily from the fact that queries have a complex structure and can be broken into smaller parts. We address these challenges through a particular instantiation of our framework focusing primarily on the optimization algorithms run on “buying” and “selling” nodes, the evaluation metrics of the queries, and the negotiation strategy. Finally, we present the results of several experiments that demonstrate the performance characteristics of our approach compared to those of traditional query optimization.
Vitamin E Isoform γ-Tocotrienol Downregulates House Dust Mite–Induced Asthma (2015)

Inflammation and oxidative damage contribute to the pathogenesis of asthma. Although corticosteroid is the first-line treatment for asthma, a subset of patients is steroid resistant, and chronic steroid use causes side effects. Because vitamin E isoform γ-tocotrienol possesses both antioxidative and anti-inflammatory properties, we sought to determine protective effects of γ-tocotrienol in a house dust mite (HDM) experimental asthma model. BALB/c mice were sensitized and challenged with HDM. Bronchoalveolar lavage (BAL) fluid was assessed for total and differential cell counts, oxidative damage biomarkers, and cytokine levels. Lungs were examined for cell infiltration and mucus hypersecretion, as well as the expression of antioxidants and proinflammatory biomarkers. Sera were assayed for IgE and γ-tocotrienol levels. Airway hyperresponsiveness in response to methacholine was measured. γ-Tocotrienol displayed better free radical-neutralizing activity in vitro and inhibition of BAL fluid total, eosinophil, and neutrophil counts in HDM mouse asthma in vivo, as compared with other vitamin E isoforms, including α-tocopherol. Besides, γ-tocotrienol abated HDM-induced elevation of BAL fluid cytokine and chemokine levels, total reactive oxygen species and oxidative damage biomarker levels, and of serum IgE levels, but it promoted lung-endogenous antioxidant activities. Mechanistically, γ-tocotrienol was found to block nuclear NF-κB level and enhance nuclear Nrf2 levels in lung lysates to greater extents than did α-tocopherol and prednisolone. More importantly, γ-tocotrienol markedly suppressed methacholine-induced airway hyperresponsiveness in experimental asthma. To our knowledge, we have shown for the first time the protective actions of vitamin E isoform γ-tocotrienol in allergic asthma.
Hair testing is taking root (2011)

An increasing number of toxicology laboratories are choosing to expand the services they offer to include hair testing in response to customer demands. Hair provides the toxicologist with many advantages over conventional matrices in that it is easy to collect, is a robust and stable matrix that does not require refrigeration, and most importantly, provides a historical profile of an individual's exposure to drugs or analytes of interest. The establishment of hair as a complementary technique in forensic toxicology is a direct result of the success of the matrix in medicolegal cases and the wide range of applications. However, before introducing hair testing, laboratories must consider what additional requirements they will need that extend beyond simply adapting methodologies already validated for blood or urine. Hair presents many challenges with respect to the lack of available quality control materials, extensive sample handling protocols and low drug concentrations requiring greater instrument sensitivity. Unfortunately, a common pitfall involves over-interpretation of the findings and must be avoided.
Metallo-Carbohedrenes: Formation of Multicage Structures (1992)

An unusual structural growth pattern has been found in the system of Zr m C n , in which multicage structures are formed. The experimental evidence shows that the first cage closes at Zr 8 C 12 . Surprisingly, subsequent cluster growth does not lead to the enlargement of the cage size as it usually does in the case of pure carbon clusters and water clusters, for example. Rather, multicage structures are developed, that is, a double cage at Zr 13 C 22 and Zr 14 C 21/23 , a triple cage at Zr 18 C 29 , and a quadruple cage at Zr 22 C 35 . This feature distinguishes the class of metallo-carbohedrenes from the regular doped fullerenes.
A micromachined electrochemical sensor for free chlorine monitoring in drinking water (2006)

In this work, we designed, fabricated and tested a disposable, flow-through amperometric sensor for free chlorine determination in water. The sensor is based on the principle of an electrochemical cell. The substrate, as well as the top microfluidic layer, is made up of a polymer material. The advantages include; (a) disposability from low cost; (b) stable operation range from three-electrode design; (c) fluidic interconnections that provide on line testing capabilities; and (d) transparent substrate which provides for future integration of on-chip optics. The sensor showed a good response and linearity in the chlorine concentration ranging from 0.3 to 1.6 ppm, which applies to common chlorination process for drinking water purification.
The Type IIs Restriction Endonuclease BspMI Is a Tetramer That Acts Concertedly at Two Copies of an Asymmetric DNA Sequence (2002)

Type IIs endonucleases recognize asymmetric DNA sequences and cleave both strands at fixed positions downstream of the sequence. Many type IIs enzymes, including BspMI, cleave substrates with two sites more rapidly than those with one site. They usually act sequentially on DNA with two sites, but BspMI converted such a substrate directly to the final products cut at both sites. The BspMI endonuclease was found to be a tetramer, in contrast to the monomeric structures for many type IIs enzymes. No change in subunit association occurred during the BspMI reaction. Plasmids with two BspMI sites were cleaved in cis, in reactions spanning sites in the same DNA, even when the sites were separated by just 38 bp. Plasmids with one BspMI site were cleaved in trans, with the enzyme bridging sites in separate DNA molecules: these slow reactions could be accelerated by adding a second DNA with the recognition sequence. Thus, whereas many type IIs enzymes dimerize before cleaving DNA, a process facilitated by two recognition sites in cis, the BspMI tetramer binds two copies of its recognition sequence before cleaving the DNA in both strands at both sites.
High-rate (∼50-Å/s) deposition of ZnO films for amorphous silicon alloy solar-cell back-reflector application (1991)

Back reflectors have been fabricated by the deposition of ZnO films on textured Ag films. High deposition rates of ∼50 Å/s have been achieved by the dc magnetron sputtering technique. The ZnO target used has been prepared in our laboratory. Amorphous silicon alloy solar cells have been deposited on the ZnO/textured Ag back reflector. Control samples have been prepared by the deposition of identical cells on the same back reflector, but in which the ZnO films have been prepared by a low-rate ∼5-Å/s rf sputtering process. The short-circuit current density, which has been used as the primary test parameter for evaluating the back reflectors, is slightly superior in the case of the high-rate ZnO back reflector. The high-rate deposition process is, therefore, attractive for large-volume production application.
Improved HCP Reduction Using a New, All‐Synthetic Depth Filtration Media Within an Antibody Purification Process (2018)

Biologic manufacturing processes typically employ clarification technologies like depth filtration to remove insoluble and soluble impurities. Conventional depth filtration media used in these processes contain naturally-derived components like diatomaceous earth and cellulose. These components may introduce performance variability and contribute extractable/leachable components like beta-glucans that could interfere with limulus amebocyte lysate endotoxin assays. Recently a novel, all-synthetic depth filtration media is developed (Millistak+<sup>®</sup> HC Pro X0SP) that may improve process consistency, efficiency, and drug substance product quality by reducing soluble process impurities. This new media is evaluated against commercially available benchmark filters containing naturally-derived components (Millistak+<sup>®</sup> HC X0HC and B1HC). Using model proteins, the synthetic media demonstrates increased binding capacity of positively charged proteins (72-126 mg g<sup>-1</sup> media) compared to conventional media (0.3-8.6 mg g<sup>-1</sup> media); and similar values for negatively charged species (1.3-5.6 mg g<sup>-1</sup> media). Several CHO-derived monoclonal antibodies (mAbs) or mAb-like molecules are also evaluated. The X0SP filtration performance behaves similarly to benchmarks, and exhibits improved HCP reduction (at least 50% in 55% of cases tested). X0SP filtrates contained increased silicon extractables relative to benchmarks, but these were readily removed downstream. Finally, the X0SP devices demonstrates suitable lot-to-lot robustness when specific media components are altered intentionally to manufacturing specification limits.