gebhart/patrepeval huggingface dataset:
Optimizing Optical Flow Cytometry for Cell Volume-Based Sorting and Analysis (2011)

Cell size is a defining characteristic central to cell function and ultimately to tissue architecture. The ability to sort cell subpopulations of different sizes would facilitate investigation at genomic and proteomic levels of mechanisms by which cells attain and maintain their size. Currently available cell sorters, however, cannot directly measure cell volume electronically, and it would therefore be desirable to know which of the optical measurements that can be made in such instruments provide the best estimate of volume. We investigated several different light scattering and fluorescence measurements in several different cell lines, sorting cell fractions from the high and low end of distributions, and measuring volume electronically to determine which sorting strategy yielded the best separated volume distributions. Since we found that different optical measurements were optimal for different cell lines, we suggest that following this procedure will enable other investigators to optimize their own cell sorters for volume-based separation of the cell types with which they work.
Implementing efficient data compression and encryption in a persistent key-value store for HPC (2019)

Recently, persistent data structures, like key-value stores (KVSs), which are stored in a high-performance computing (HPC) system’s nonvolatile memory, provide an attractive solution for a number of emerging challenges like limited I/O performance. Data compression and encryption are two well-known techniques for improving several properties of such data-oriented systems. This article investigates how to efficiently integrate data compression and encryption into persistent KVSs for HPC with the ultimate goal of hiding their costs and complexity in terms of performance and ease of use. Our compression technique exploits deep memory hierarchy in an HPC system to achieve both storage reduction and performance improvement. Our encryption technique provides a practical level of security and enables sharing of sensitive data securely in complex scientific workflows with nearly imperceptible cost. We implement the proposed techniques on top of a distributed embedded KVS to evaluate the benefits and costs of incorporating these capabilities along different points in the dataflow path, illustrating differences in effective bandwidth, latency, and additional computational expense on Swiss National Supercomputing Centre’s Grand Tavé and National Energy Research Scientific Computing Center’s Cori.
An evaluation of albumin microcapsules prepared using a multiple emulsion technique (1987)

Albumin microcapsules containing sulphadiazine were prepared using a multiple emulsion technique. Heat was utilized to denature the albumin and form the capsule shell. Albumin microcapsules prepared using this technique were free-flowing, spherical in shape, and had varying degrees of vacuolation. The effects of drug: polymer ratio and concentration of cross-linking agent on the percentage of drug retained in the microcapsules and release of drug from the microcapsules were studied. Also, the effect of viscosity of the innermost oil layer, of the multiple emulsion, upon the mean diameter of the microcapsules and release of drug from the microcapsules was investigated.
The geometric dynamic channel allocation as a practical strategy in mobile networks with bursty user mobility (1995)

In this paper we refer to a specific class of Dynamic Channel Allocation (DCA) strategies, namely the inferferencefree, timid, not-conditioned class. The main concern of this work is to verify if and to what extent strategies belonging to this class can offer better performance than Fixed Channel Allocation (FCA). The interest in this kind of strategies is motivated by their feasibility with current TDM technologies, the limited amount of information required to carry out channel assignments and their intrinsic stability. In this framework we present a simple, but very attractive DCA strategy, the so-called Geometric DCA (GDCA). A performance evaluation is carried out to compare some representative DCA strategies of the considered class, by using a user mobility model that accounts for the large fluctuations of the number of users in a cell coverage area expected in a microcellular environment. The effect of the non-null propagation time required by the information exchange in the DCA strategies is also taken into account. It emerges that the proposed GDCA allows better performance than more sophisticated strategies already proposed, at the expense of a frequency planning carried out only at network configuration. This is due to the ability of GDCA to exploit the a priori information to maintain a tight geometric packing of used carriers. The reported results also show that DCA strategies in the considered class cope with large and sudden traffic fluctuations remarkably better than the FCA scheme does and that the advantage becomes more evident as the burstiness of the user mobility process (hence of the offered traffic) increases.
Automatic program verification of continuous function chart based on model checking (2009)

Continuous function chart (CFC) is a graphical-oriented programming language that is widely used as application programs in process industry (e.g. pharmaceutical plants, chemical plants and power plants). The CFC programs have to be validated whether they fulfill the safety requirements that are specified in the control task specifications to verify their correctness before they are being tested by user in the plant. Nowadays, this verification is being done manually. This potentially causes errors and it is very time consuming. In this paper, we present an automatic verification approach to ensure the correctness of the CFC programs. Our verification approach is based on model checking where the CFC programs are modeled into timed automata.
RelA Protein Is Involved in Induction of Genetic Competence in Certain<i>Bacillus subtilis</i>Strains by Moderating the Level of Intracellular GTP (2002)

We found that the ability to develop genetic competence of a certain relaxed (relA) aspartate-auxotrophic strain of Bacillus subtilis is significantly lower than that of the isogenic stringent (relA+) strain. Transcriptional fusion analysis utilizing a lacZ reporter gene indicated that the amount of the ComK protein, known as the key protein for competence development, is greatly reduced in the relaxed strain than in the stringent strain. We also found that the addition of decoyinine, a GMP synthetase inhibitor, induces expression of a competence gene (comG) in the relaxed strain, accompanied by a pronounced decrease in the level of intracellular GTP as measured by high-performance liquid chromatography. The transformation efficiency of the relaxed strain increased 100-fold when decoyinine was added at t0 (the transition point between exponential to stationary growth phase). Conversely, supplementation of guanosine together with decoyinine completely abolished the observed effect of adding decoyinine on competence development. Furthermore, the impaired ability of the relaxed strain for competence development was completely restored by disrupting the codY gene, which is known to negatively control comK expression. Our results indicate that the RelA protein plays an essential role in the induction of competence development at least under certain physiological conditions by reducing the level of intracellular GTP and overcoming CodY-mediated regulation.
THE HIGH TEMPERATURE OXIDATION OF ZIRCALOY IN WATER (1954)

boron to the Zircaloy-2 or Zircaloy-3 base fuel alloys markedly affects the response of corrosion resistance with prior thermal history. Zircaloy-2 uranium-- boron alloys exhibit the best corrosion properties after fast cooling from high (950 deg C) temperature, while the low- temperature (550 deg C) treatment results in the most corrosion resistant forms of the Zircaloy-3-uranium-boron alloys. (auth)
Rapid neural differentiation of human cord blood-derived mesenchymal stem cells (2004)

Human umbilical cord blood (UCB) contains hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs), both of which are regarded as valuable sources for cell transplantation and cell therapy. Adherent cells expressing MSCs-related antigens such as SH2, CD13, CD29, and ASMA, have been isolated from a mononuclear cell fraction of human UCB. Under proneurogenic conditions, these UCB-derived adherent cells rapidly assumed the morphology of multipolar neurons. Both immunofluorescence and RT-PCR analyses indicated that the expression of a number of neural markers including Tuj1, TrkA, GFAP and CNPases, was markedly elevated during this acute differentiation. The neurogenic potential of UCB-derived may facilitate stem cell therapeutic approaches to neurodegenerative diseases.
Mechanics of Forward Flight in Bumblebees: I. Kinematics and Morphology (1990)

ABSTRACT Using high-speed cinematography, bumblebees in free flight were filmed over a range of forward airspeeds. A detailed description of the wing tip and body kinematics was obtained from a three-dimensional reconstruction of the twodimensional film image. A technique for determining quantitatively the angle of attack of the wing was developed. Kinematic parameters found to vary consistently with airspeed were body angle, stroke plane angle, geometrical angle of attack, and rotational angles of the wings at the ends of half-strokes. Results of a morphological analysis of the wings and bodies of those insects filmed in free flight are presented for use in later calculations of the lift and power requirements of forward flight.
<title>EELV Secondary Payload Adapter (ESPA)</title> (2000)

Despite growing international interest in small satellites, high dedicated expendable launch vehicle costs and the lack of secondary launch opportunities continue to hinder the full exploitation of small satellite technology. In the United States, the Department of Defense (DoD), NASA, other government agencies, commercial companies, and many universities use small satellites to perform space experiments, demonstrate new technology, and test operational prototype hardware. In addition, the DoD continues to study the role of small satellites in fulfilling operational mission requirements. However, the US lacks sufficient small satellite launch capacity. Furthermore, US government agencies are restricted to the use of US launch vehicles, which eliminates many affordable launch opportunities. In an effort to increase the number of space experiments that can be flown with a small, fixed budget, the DoD Space Test Program (STP) has teamed with the Air Force Research Laboratory Space Vehicles Directorate (AFRL/VS) to develop a low-cost solution for the small satellite launch program. Our solution, which can be implemented on both Boeing and Lockheed-Martin Evolved Expendable Launch Vehicle-Medium (EELV-M) boosters, is called the EELV Secondary Payload Adaptor (ESPA). ESPA will increase the number of launch opportunities for 180kg-class (or smaller) satellites at prices highly competitive with other secondary launch services worldwide.
Expression of angiogenic and immunosuppressive factors by uveal melanoma cell lines (1999)

Dissemination of uveal melanomas is almost exclusively haematogenous, making angiogenesis of the tumour a prerequisite for the formation of metastases. Uveal melanomas must employ strategies to evade the immune system in order to escape immune surveillance. We therefore determined the expression of the following angiogenic and immunosuppressive factors in seven human uveal melanoma cell lines using reverse transcriptase-polymerase chain reaction (RT-PCR): secreted interleukin-1 receptor antagonist (slL-1ra), interleukin (IL)-6, IL-8, IL-10, transforming growth factor (TGF)-α, TGFβ, vascular endothelial growth factor (VEGF), platelet derived growth factor (PDGF), basic fibroblast growth factor (bFGF), angiopoietin-1 and angio-poietin-2. In addition, the secretion of slL-1ra, IL-6, IL-8, IL-10, TGFβ and VEGF was assayed by enzyme linked immunosorbent assay (ELISA). The potential of uveal melanoma cell lines to convert plasminogen to angiostatin was tested in an in vitro assay. All the factors except angiopoietin-1 were determined in one or more cell lines using RT-PCR, although these results were not necessarily confirmed by ELISA. Expression of VEGF and angiopoietin-2 was found in all seven cell lines. Production of angiostatin was observed in one cell line. All seven cell lines examined expressed angiogenic factors and most cell lines expressed immunosuppressive factors. The expression of VEGF and angiopoietin-2 in combination with a lack of angiopoietin-1 expression suggest high vascular remodelling capacity and could be of great relevance for the metastatic potential of uveal melanoma.
Recent Advancement in the Treatment of Open Apex: Materials and Technique (2012)

Cessation of the root development occurred by traumatic injury and as a sequence of caries prevents obturation of the root canal during endodontic treatment and subsequent treatment like fixed prosthesis. Thus, the goal of apexification was to obtain an apical barrier to prevent the passage of toxins and bacteria into the periapical tissues from the root canal and to induce an apical barrier against which GP points can be condensed. Apexification has been carried out from long time where calcium hydroxide is the widely used material. Calcium hydroxide gained the widest popularity. It is bactericidal in nature with an alkaline pH that may be responsible for stimulating apical calcification. Despite its widest acceptance, it has some disadvantages including variability of treatment time, difficulty in patients follow up, delayed healing, needs multiple visits to the completion to induction of calcific barrier formation and it weakens the dentin of root. Thus, the scientists and clinicians felt urge to introduce new materials and techniques that will help to the quick resolution of the apical radiolucency and to form the apical barrier. MTA (mineral trioxide aggregate), a recently introduced material is successfully used in the treatment of apexification. Many renowned scientists and clinicians worked on this materials and got very good results. Recently a clinical study has been published showing the effects of a combined medicament prepared by mixing calcium hydroxide with cefadroxil on the resolution and formation of apical barrier. Although it was a multi visits treatment compared to the treatment with MTA but showed promising results in the treatment of apexification in presence or absence of radiolucency. Thus, this review article will expose the recently invented materials and techniques which will provide a helpful guide for the clinicians to perform apexification. This review article has been prepared from the internet information and reviewing the recently published papers and texts. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12320 City Dental College J. Volume-9, Number-2, July-2012
Robust estimation of cyclic correlation in contaminated Gaussian noise (2002)

This paper considers the effect of non-Gaussian noise on the conventional estimate of cyclic correlation. It is shown that noise having a distribution function with heavier tails than the Gaussian slows the convergence of the estimate to the expected value. Alternative estimators are proposed based on the statistical concepts of robustness. These alternative estimators are shown via Monte Carlo simulation to perform well in both Gaussian and non-Gaussian noise. Another contribution of this paper is the generalization of some robust estimators to complex (versus real) data.
In situ transmission electron microscopy investigation of Ce(iv) and Pr(iv) reducibility in a Rh (1%)/Ce0.8Pr0.2O2–x catalyst (2003)

In-situ Atomic Resolution Transmission Electron Microscopy studies carried out on a Rh/Ce0.8Pr0.2O(2-x) catalyst, under hydrogen in the temperature range 298-1223 K, show the occurrence of consecutive reduction of Pr4+ and Ce4+ ions, and the formation of an oxygen-deficient Ln16O30 (Ln: Ce, Pr) ordered phase.
Ethics and animal numbers: informal analyses, uncertain sample sizes, inefficient replications, and type I errors. (2011)

To obtain approval for the use vertebrate animals in research, an investigator must assure an ethics committee that the proposed number of animals is the minimum necessary to achieve a scientific goal. How does an investigator make that assurance? A power analysis is most accurate when the outcome is known before the study, which it rarely is. A 'pilot study' is appropriate only when the number of animals used is a tiny fraction of the numbers that will be invested in the main study because the data for the pilot animals cannot legitimately be used again in the main study without increasing the rate of type I errors (false discovery). Traditional significance testing requires the investigator to determine the final sample size before any data are collected and then to delay analysis of any of the data until all of the data are final. An investigator often learns at that point either that the sample size was larger than necessary or too small to achieve significance. Subjects cannot be added at this point in the study without increasing type I errors. In addition, journal reviewers may require more replications in quantitative studies than are truly necessary. Sequential stopping rules used with traditional significance tests allow incremental accumulation of data on a biomedical research problem so that significance, replicability, and use of a minimal number of animals can be assured without increasing type I errors.
Traf2- and Nck-Interacting Kinase Is Essential for Wnt Signaling and Colorectal Cancer Growth (2010)

T-cell factor-4 (TCF4) is a transcription factor essential for maintaining the undifferentiated status and self-renewal of intestinal epithelial cells. It has therefore been considered that constitutive activation of TCF4 by aberrant Wnt signaling is a major force driving colorectal carcinogenesis. We previously identified Traf2- and Nck-interacting kinase (TNIK) as one of the proteins that interact with TCF4 in colorectal cancer cells, but its functional significance has not been elucidated. Here, we report that TNIK is an activating kinase for TCF4 and essential for colorectal cancer growth. TNIK, but not its catalytically inactive mutant, phosphorylated the conserved serine 154 residue of TCF4. Small interfering RNA targeting TNIK inhibited the proliferation of colorectal cancer cells and the growth of tumors produced by injecting colorectal cancer cells s.c. into immunodeficient mice. The growth inhibition was abolished by restoring the catalytic domain of TNIK, thus confirming that its enzyme activity is essential for the maintenance of colorectal cancer growth. Several ATP-competing kinase inhibitors have been applied to cancer treatment and have shown significant activity. Our findings suggest TNIK as a feasible target for pharmacologic intervention to ablate aberrant Wnt signaling in colorectal cancer.
Work Hazard Control Efforts by Wisconsin Dairy Farmers (1996)

<em>Objective:</em> Better information is needed about how farm operators view agriculturalwork hazards and prevention efforts. Agricultural work safety efforts of Wisconsinfarmers were examined, along with their perceptions of the farm safety problem.<br><br><em>Methods:</em> A survey instrument was designed and administered to an opportunitysample of 401 dairy farmers using a standardized mail survey technique.<br><br><em>Results:</em> Usable questionnaires were obtained from 268 farm operators(67% response rate). Most farm operators did annual self-inspections, wore seatbelts inmotor vehicles, and avoided extra riders on tractors. Most wanted to obtain and useinspection checklists and were willing to commit monetary resources to makingcorrections. However, most dairy farmers had no interest in Extension safety programsand had attended none in the last year. The farmers did want Extension agents tofacilitate self-inspection and hazard corrections.<br><br><em>Conclusion:</em> Self-help tools to assist farmers in recognizing, identifying, andcontrolling hazards are a promising, low intensity intervention for reducing injury anddisease. Extension agents are in a strong position to abet farmer self inspections.
Query optimization and processing in federated database systems (1993)

Article Free Access Share on Query optimization and processing in federated database systems Authors: Ee-Peng Lim Department of Computer Science, University of Minnesota, Minneapolis, MN Department of Computer Science, University of Minnesota, Minneapolis, MNView Profile , Jaideep Srivastava Department of Computer Science, University of Minnesota, Minneapolis, MN Department of Computer Science, University of Minnesota, Minneapolis, MNView Profile Authors Info & Claims CIKM '93: Proceedings of the second international conference on Information and knowledge managementDecember 1993 Pages 720–722https://doi.org/10.1145/170088.170469Online:01 December 1993Publication History 5citation658DownloadsMetricsTotal Citations5Total Downloads658Last 12 Months18Last 6 weeks8 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Experimental Implementation of Frequency Regulation Services Using Commercial Buildings (2016)

This paper illustrates the potential of commercial buildings to act as frequency reserves providers through an experimental demonstration conducted in a multi-zone university building. The proposed control methodology is presented in detail, including the control architecture, the controller design, model identification, and hardware description. Finally, the effectiveness of the presented approach is tested by means of simulations and experiments in a controlled environment.
Structural determinants and specificities for ROMK1-phosphoinositide interaction (2002)

We have recently reported that direct interaction between phosphatidylinositol bisphosphate (PIP(2)) and the COOH-terminal cytoplasmic domain of ROMK1 is important for opening of the channel. We identified arginine-188 of ROMK1 as a critical residue for this interaction. Here, we further report that substitution of a neutral amino acid for lysine-181, arginine-217, or lysine-218 decreases single-channel open probability for the full-conductance state and increases the frequency of opening at a subconductance state. Compared with wild-type ROMK1 channels, these substitution mutants also display an increased sensitivity to the block by anti-PIP(2) antibodies and to inhibition by intracellular protons. These results indicate that, like arginine-188, lysine-181, arginine-217, and lysine-218 are also involved in interactions with PIP(2) and are critical for ROMK1 to open at full conductance. Using synthetic phosphoinositides containing phosphates at different positions in the head group, we also examined the specificities of phosphoinositides in the regulation of ROMK1 channels. We found that phosphoinositides containing phosphate at both positions 4 and 5 of the inositol head group have the highest efficacy in activating ROMK1 channels. These results suggest that phosphatidylinositol 4,5-bisphosphate is likely the important phosphoinositide in the regulation of ROMK1 channels in a physiological membrane milieu.