gebhart/patrepeval huggingface dataset:
<i>Desulfovibrio vulgaris</i> bacterioferritin uses H2O2 as a co-substrate for iron oxidation and reveals DPS-like DNA protection and binding activities (2012)

A gene encoding Bfr (bacterioferritin) was identified and isolated from the genome of Desulfovibrio vulgaris cells, and overexpressed in Escherichia coli. In vitro, H(2)O(2) oxidizes Fe(2+) ions at much higher reaction rates than O(2). The H(2)O(2) oxidation of two Fe(2+) ions was proven by Mössbauer spectroscopy of rapid freeze-quenched samples. On the basis of the Mössbauer parameters of the intermediate species we propose that D. vulgaris Bfr follows a mineralization mechanism similar to the one reported for vertebrate H-type ferritins subunits, in which a diferrous centre at the ferroxidase site is oxidized to diferric intermediate species, that are subsequently translocated into the inner nanocavity. D. vulgaris recombinant Bfr oxidizes and stores up to 600 iron atoms per protein. This Bfr is able to bind DNA and protect it against hydroxyl radical and DNase deleterious effects. The use of H(2)O(2) as an oxidant, combined with the DNA binding and protection activities, seems to indicate a DPS (DNA-binding protein from starved cells)-like role for D. vulgaris Bfr.
MMSE-Based Packet Loss Concealment for CELP-Coded Speech Recognition (2009)

In this paper, we analyze the performance of network speech recognition (NSR) over IP networks, adapting and proposing new solutions to the packet loss problem for code excited linear prediction (CELP) codecs. NSR has a client-server architecture which places the recognizer at the server side using a standard speech codec for speech transmission. Its main advantage is that no changes are required for the existing client devices and networks. However, the use of speech codecs degrades its performance, mainly in the presence of packet losses. First, we study the degradations introduced by CELP codecs in lossy packet networks. Later, we propose a reconstruction technique based on minimum mean square error (MMSE) estimation using hidden Markov models. This approach also allows us to obtain reliability measures associated to each estimate. We show how to use this information to improve the recognition performance by means of soft-data decoding and weighted Viterbi algorithm. The experimental results are obtained for two well-known CELP codecs, G.729 and AMR 12.2 kbps, carrying out recognition from decoded speech. Finally, we analyze an efficient and improved implementation of the proposed techniques using an NSR system which extracts speech recognition features directly from the bit-stream parameters. The experimental results show that the different proposed NSR systems achieve a comparable performance to distributed speech recognition (DSR).
The Effect of Refrigerants on the Lubrication of Rolling Element Bearings Used in Screw Compressors (1992)

The effect of refrigerants on the lubrication of rolling element bearings used in screw compressors
A Low Loss Flexible Stripline For 1.9ghz Antenna (1997)

A new flexible stripline for microwave transmission line has been developed. This stripline has lower profile and lower loss than a coaxial cable which has same thickness. The transmission line loss at lGHz of 0.6” thickness and 0.3” thickness stripline are 1.7dB/m and 3.5dBim respectively. These are approximately 10% lower loss than that of a coaxial cable which has same thickness. As for an application of the new stripline, 1.9GHz antenna was designed, which consists of a transmission part and a printed dipole. The measured average horizontal gain is -3dBd when the antenna is fixed in a portable telephone. This results show that this antenna is very suitable for PHS.
Text-dependent speaker identification using circular hidden Markov models (2003)

A brief overview of hidden Markov models is given. The properties of circular hidden Markov models and their application to speaker recognition are discussed. For each person, a distinct reference CHMM is produced using the Baum forward-and-backward algorithm. Classification is carried out by selecting the model with the highest probability as the speaker identification system output. Preliminary testing on a set of ten speakers indicates a performance of about 94% speaker recognition accuracy.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A survey on scheduling and load balancing techniques in cloud computing environment (2014)

Now-a-days cloud computing is the most emerging technology due to its elasticity of resource provisioning and the pay-as-you-go pricing model which enables users to pay only according to their need. As cloud can be accessed anytime and anywhere through commodity hardware only its demand is increasing day by day. So it must provide high performance gain to the user and at the same time must be beneficial for the Cloud Service Provider (CSP). To achieve this goal many challenges have to be faced. Load balancing is one of them which helps the CSP to meet the QoS requirements of the users and at the same time maximize his profit by optimum use of the resources. To balance the load in cloud the resources and workloads must be scheduled in an efficient manner. A variety of scheduling algorithms are used by load balancers to determine which backend server to send a request to. The selected server allocates resources and schedules the job dynamically on some virtual machine (VM) located on the same physical machine. It is also the responsibility of the provider to dynamically reallocate or migrate the VM across physical machines for workload consolidation and to avoid over utilization or under utilization of resources. In this paper, we have discussed different algorithms proposed to resolve the issue of load balancing and task scheduling in Cloud Computing. We have mentioned some of their shortcomings for further development. VM migration issues involved in load balancing are also described briefly.
Use New Diagnosis Codes to Avoid Claim Denials (1998)

This article updates readers on additions to and deletions from the ICD-9 codes effective in 1999.
SOP: Adaptive Massively Parallel System (1995)

this paper, we present basic architecture of adaptive massively parallel computer system SOP, data structures and mapping methods with various novel features. These features are 1) directly mapped and hardwired application program, 2) extraction of high degree of parallelism by exploiting operation and function level parallelism, 3) pipelined loop operation, 4) hardwired communication and synchronization, 5) clock level scheduling compiler, 6) explicit parallelism description, 7) large data structure manipulation with functional memory.
Method to implement packet level access control in multinetworks (1989)

The letter describes a method to implement an access control policy for multinetwork environments. The access control method is based on the public-key cryptosystem proposed by Rivest, Shamir and Adleman (RSA) and on the message authentication algorithm based on Data Encryption Standard (DES)
Expression of 25-Hydroxyvitamin D<sub>3</sub>-24-Hydroxylase Activity in Caco-2 Cells, An<i>in Vitro</i>Model of Intestinal Vitamin D Catabolism* (1990)

The C-24 oxidation pathway plays a major role in the degradation of vitamin D metabolites in kidney and other target tissues. The aim of the present study was to establish an intestinal cell culture system to study the mechanisms regulating the vitamin D catabolic pathway. 25-Hydroxyvitamin D3-24-hydroxylase (24-hydroxylase), the first enzyme in the catabolic sequence, was examined in Caco-2 cells, a human colon adenocarcinoma cell line which exhibits differentiated functions of absorbing intestinal epithelial cells. While untreated Caco-2 cells did not exhibit 24-hydroxylase activity, significant catabolic activity was induced by prior treatment of cell monolayers with 1,25-dihydroxyvitamin D3(1,25-(OH)2D3). Induced 24-hydroxylase D3 (25OHD3) and 1,25-(OH)2D3 was detected 6 h after treatment of cells with 10(-8)M 1,25-(OH)2D3, peaked at 16 h, and decreased thereafter. Treatment of cells with 10(-7) M 1,25-(OH)2D3 elicited a maximal 24-hydroxylase response. Comparable time courses of induction by 1,25-(OH)2D3 and 1,25-(OH)2D3-dose response curves were observed in cultured human skin fibroblasts and Caco-2 cells. 25OHD3 was not as good an inducer of the vitamin D catabolic pathway in Caco-2 cells as 1,25-(OH)2D3. Induction of 24-hydroxylase activity by 1,25-(OH)2D3 was inhibited by pretreatment of Caco-2 cells with either actinomycin D, alpha-amanitin, or cycloheximide suggesting that mRNA and protein synthesis are required for induction. The present study demonstrates that 1,25-(OH)2D3-treated Caco-2 cells express the vitamin D catabolic pathway and, therefore, constitute a useful in vitro model to study the mechanism of induction by 1,25-(OH)2D3.
Low dark current dual band infrared photodetector using thin AlAs barriers and Γ-<i>X</i> mixed intersubband transition in GaAs quantum wells (1994)

A low dark current dual band quantum well infrared photodetector is demonstrated by adding thin AlAs barriers to the usual detector structure, which consists of Si-doped GaAs wells separated by thick AlGaAs barriers. The advantages of adding the thin AlAs barriers to clad the quantum wells are that (a) the detector displays a low dark current and (b) intersubband photocurrents result from transitions from both the Γ ground to the first excited state, and from the Γ ground to a mixed Γ-X excited state because the X-valley band edge forms a well in AlAs and intrinsic Γ-X mixing occurs. The spectral peaks of these two transitions, which occur at 8.5 and 5.5 μm in our test structure, can be varied by changing device parameters during growth.
Role of the IL-23/IL-17 Axis in Psoriasis and Psoriatic Arthritis: The Clinical Importance of Its Divergence in Skin and Joints (2018)

Psoriasis is a chronic systemic inflammatory disease causing erythematosus and scaly skin plaques; up to 30% of patients with psoriasis develop Psoriatic Arthritis (PsA), which is characterised by inflammation and progressive damage of the peripheral joints and/or the spine and/or the entheses. The pathogenic mechanisms driving the skin disorder in psoriasis and the joint disease in PsA are sustained by the activation of inflammatory pathways that can be overlapping, but also, at least partially, distinct. Cytokines members of the IL-23/IL-17 family, critical in the development of autoimmunity, are abundantly expressed within the cutaneous lesions but also seem to be involved in chronic inflammation and damage of the synovium though, as it will be here discussed, not in all patients. In this review, we will focus on the state of the art of the molecular features of psoriatic skin and joints, focusing on the specific role of the IL-23/IL-17 pathway in each of these anatomical districts. We will then offer an overview of the approved and in-development biologics targeting this axis, emphasising how the availability of the "target" in the diseased tissues could provide a plausible explanation for the heterogeneous clinical efficacy of these drugs, thus opening future perspective of personalised therapies.
Density-Permittivity Relationships for Powdered and Granular Materials (2005)

Relationships between the permittivities of powdered or granular solid materials and their bulk densities (density of the air-particle mixture) are discussed. Linear relationships between functions of the permittivity and bulk density are identified that are useful in determining permittivity of solids from measurements of the permittivity of pulverized samples. The usefulness of several dielectric mixture equations for calculating solid material permittivity from measured permittivity of pulverized samples is also discussed. Results of testing linear extrapolation techniques and dielectric mixture equations on pulverized coal, limestone, plastics, and granular wheat and flour are presented. Recommendations are provided for reliable estimation of solid material permittivities or changes in permittivities of powdered and granular materials as a result of changes in their bulk densities.
Can This Brokered Marriage Be Saved? The Changing Relationship Between the Supreme Court and Federal Circuit in Patent Law Jurisprudence, 2 J. Marshall Rev. Intell. Prop. L. 201 (2003) (2003)

Congress created the Federal Circuit, in part, to provide uniformity in patent decisions throughout the United States and stability in patent law. During the first decade of the Federal Circuit’s existence, the Supreme Court largely deferred to the Federal Circuit in patent law decisions. However, the Supreme Court’s initial deference to the Federal Circuit has since been replaced by critical view of the Federal Circuit’s decisions and its decision-making processes. This article proposes that the Supreme Court has correctly abandoned its deferential mindset toward the Federal Circuit since the Federal Circuit was never intended to be the de facto Supreme Court for patent issues.
EMG-Driven Forward-Dynamic Estimation of Muscle Force and Joint Moment about Multiple Degrees of Freedom in the Human Lower Extremity (2012)

This work examined if currently available electromyography (EMG) driven models, that are calibrated to satisfy joint moments about one single degree of freedom (DOF), could provide the same musculotendon unit (MTU) force solution, when driven by the same input data, but calibrated about a different DOF. We then developed a novel and comprehensive EMG-driven model of the human lower extremity that used EMG signals from 16 muscle groups to drive 34 MTUs and satisfy the resulting joint moments simultaneously produced about four DOFs during different motor tasks. This also led to the development of a calibration procedure that allowed identifying a set of subject-specific parameters that ensured physiological behavior for the 34 MTUs. Results showed that currently available single-DOF models did not provide the same unique MTU force solution for the same input data. On the other hand, the MTU force solution predicted by our proposed multi-DOF model satisfied joint moments about multiple DOFs without loss of accuracy compared to single-DOF models corresponding to each of the four DOFs. The predicted MTU force solution was (1) a function of experimentally measured EMGs, (2) the result of physiological MTU excitation, (3) reflected different MTU contraction strategies associated to different motor tasks, (4) coordinated a greater number of MTUs with respect to currently available single-DOF models, and (5) was not specific to an individual DOF dynamics. Therefore, our proposed methodology has the potential of producing a more dynamically consistent and generalizable MTU force solution than was possible using single-DOF EMG-driven models. This will help better address the important scientific questions previously approached using single-DOF EMG-driven modeling. Furthermore, it might have applications in the development of human-machine interfaces for assistive devices.
Efficient Transfection of Primary Cells Relevant for Cardiovascular Research by nucleofection<sup>®</sup> (2006)

Cell types that are important for cardiovascular research, e.g., cardiomyocytes, endothelial cells, or adult stem cells, are often hard to isolate, culture, and transfect. Low-transfection efficiencies are a major limitation because, in many cases, results achieved with surrogate model cell lines, if any at all are available for the primary cell type of interest, do not reflect the situation in the primary cell. We have demonstrated that unprecedented transfection results are achieved with primary cells when novel electroporation conditions are combined with a treatment of the cells in specific solutions that help stabilize the cells in the electrical field. This led to the development of the new proprietary transfection technology nucleofection. Nucleofection has proved to be successfully applicable to a variety of primary cells and other hard-to-transfect cell lines, and, thus, opens unique perspectives for novel experimental setups as therapeutic strategies. Herein we present protocols for the efficient nucleofection of human umbilical vein endothelial cells, human coronary artery endothelial cells, smooth muscle cells (e.g., pig vascular smooth muscle cells), neonatal rat cardiomyocytes, and human mesenchymal stem cells and depict some results obtained with such transfected cells.
Validity of the CogState Brief Battery: Relationship to Standardized Tests and Sensitivity to Cognitive Impairment in Mild Traumatic Brain Injury, Schizophrenia, and AIDS Dementia Complex (2009)

This study examined the validity of the four standard psychological paradigms that have been operationally defined within the CogState brief computerized cognitive assessment battery. Construct validity was determined in a large group of healthy adults. CogState measures of processing speed, attention, working memory, and learning showed strong correlations with conventional neuropsychological measures of these same constructs (r's = .49 to .83). Criterion validity was determined by examining patterns of performance on the CogState tasks in groups of individuals with mild head injury, schizophrenia, and AIDS dementia complex. Each of these groups was impaired on the CogState performance measures (Cohen's d's = -.60 to -1.80) and the magnitude and nature of this impairment was qualitatively and quantitatively similar in each group. Taken together, the results suggest that the cognitive paradigms operationally defined in the CogState brief battery have acceptable construct and criterion validity in a neuropsychological context.
The effect of sulphate and molybdenum on copper metabolism in cattle (1959)

Summary Jersey cattle were fed from yearling stage for 2 years on a ration low in copper and molybdenum, and containing 0.1 % of inorganic sulphate; supplements of these substances were dosed to different groups. Additional molybdenum increased blood and liver molybdenum and decreased blood and liver copper. Inorg:lnic sulphate in addition to molybdenum prevented accumulation of molybdenum in blood or liver, but did not increa,e the loss of copper from blood or liver. It was concluded that any interaction there m:ly be between molybdenum and sulphate on copper was provided for by the 0.1 % inorganic sulphate in the basal ration. It is suggested that the sensitivity of cattle to high molybdenum is related to the low level of accompanying inorganic sulphate that is necessary to cause loss of copper.
Protocol for a double-blind randomised placebo-controlled trial of lithium carbonate in patients with amyotrophic Lateral Sclerosis (LiCALS) [Eudract number: 2008-006891-31] (2011)

Current controlled trials ISRCTN83178718.
Effect of calcium ions on the activity, heat stability, and structure of trypsin (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of calcium ions on the activity, heat stability, and structure of trypsinTibor Sipos and Joseph R. MerkelCite this: Biochemistry 1970, 9, 14, 2766–2775Publication Date (Print):July 7, 1970Publication History Published online1 May 2002Published inissue 7 July 1970https://pubs.acs.org/doi/10.1021/bi00816a003https://doi.org/10.1021/bi00816a003research-articleACS PublicationsRequest reuse permissionsArticle Views3312Altmetric-Citations158LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development life-cycle WITH reuse (1994)

This article presents preliminary results on development methods carried out in the REBOOT 1 (REuse Based on Object-Oriented Techniques) ESPRIT project. It describes an adaptation of the classical object-oriented development process to development with reuse. We focus on the WITH reuse development aspects corresponding to the development of software from existing building blocks or reusable components.
Genetic Events That Limit the Efficacy of MEK and RTK Inhibitor Therapies in a Mouse Model of KRAS-Driven Pancreatic Cancer (2015)

Mutated KRAS (KRAS*) is a fundamental driver in the majority of pancreatic ductal adenocarcinomas (PDAC). Using an inducible mouse model of KRAS*-driven PDAC, we compared KRAS* genetic extinction with pharmacologic inhibition of MEK1 in tumor spheres and in vivo. KRAS* ablation blocked proliferation and induced apoptosis, whereas MEK1 inhibition exerted cytostatic effects. Proteomic analysis evidenced that MEK1 inhibition was accompanied by a sustained activation of the PI3K-AKT-MTOR pathway and by the activation of AXL, PDGFRa, and HER1-2 receptor tyrosine kinases (RTK) expressed in a large proportion of human PDAC samples analyzed. Although single inhibition of each RTK alone or plus MEK1 inhibitors was ineffective, a combination of inhibitors targeting all three coactivated RTKs and MEK1 was needed to inhibit proliferation and induce apoptosis in both mouse and human low-passage PDAC cultures. Importantly, constitutive AKT activation, which may mimic the fraction of AKT2-amplified PDAC, was able to bypass the induction of apoptosis caused by KRAS* ablation, highlighting a potential inherent resistance mechanism that may inform the clinical application of MEK inhibitor therapy. This study suggests that combinatorial-targeted therapies for pancreatic cancer must be informed by the activation state of each putative driver in a given treatment context. In addition, our work may offer explanative and predictive power in understanding why inhibitors of EGFR signaling fail in PDAC treatment and how drug resistance mechanisms may arise in strategies to directly target KRAS.
Wnt/β-catenin signaling regulates the expression of the homeobox gene <i>Cdx1</i> in embryonic intestine (2000)

During mammalian development, the Cdx1 homeobox gene exhibits an early period of expression when the embryonic body axis is established, and a later period where expression is restricted to the embryonic intestinal endoderm. Cdx1 expression is maintained throughout adulthood in the proliferative cell compartment of the continuously renewed intestinal epithelium, the crypts. In this study, we provide evidence in vitro and in vivo that Cdx1 is a direct transcriptional target of the Wnt/(beta)-catenin signaling pathway. Upon Wnt stimulation, expression of Cdx1 can be induced in mouse embryonic stem (ES) cells as well as in undifferentiated rat embryonic endoderm. Tcf4-deficient mouse embryos show abrogation of Cdx1 protein in the small intestinal epithelium, making Tcf4 the likely candidate to transduce Wnt signal in this part of gut. The promoter region of the Cdx1 gene contains several Tcf-binding motifs, and these bind Tcf/Lef1/(beta)-catenin complexes and mediate (beta)-catenin-dependent transactivation. The transcriptional regulation of the homeobox gene Cdx1 in the intestinal epithelium by Wnt/(beta)-catenin signaling underlines the importance of this signaling pathway in mammalian endoderm development.