gebhart/patrepeval huggingface dataset:
Epidermal Growth Factor (Urogastrone) in Human Tissues* (1979)

Human epidermal growth factor (hEGF), which stimulates the growth of a variety of tissues, was first isolated from mouse submandibular glands, but is also excreted in large amounts (about 50 μg/day) in human urine and is probably identical to human β-urogastrone (hUG), a potent inhibitor of stimulated gastric acid secretion. However, the primary tissue source of hEGF/hUG is as yet unknown. The hEGF/hUG in homogenates of human salivary glands and a wide variety of other endocrine and nonendocrine tissues was extracted by Amberlite CG-50 cation exchange chromatography and immune affinity chromatography using the immunoglobulin fraction of rabbit anti-hEGF serum covalently bound to agarose. The extracts were subjected to homologous hEGF RIA. Immunoreactive hEGF was found in extracts of adult submandibular gland, thyroid gland, duodenum, jejunum, and kidney, but not in several fetal tissues. The tissue immunoreactive hEGF was similar to standard hEGF in terms of immunoreactivity and elution from Sephadex G-50 Fine resin, but its concentrations were very low (1.3-5.5 ng/g wet tissue). Thus, it is not certain that these tissues represent the only source of the large amounts of hEGF/hUG that appear to be filtered by the kidneys each day. (J Clin Endocrinol Metab48: 667, 1979)
An Adaptive Load Balanced on Demand Routing Protocol (2009)

Mobile ad hoc networks are a type of mobile network that functions without any fixed infrastructure. Routing protocols are vital for the proper functioning of ad hoc networks. In the present routing protocols, data is transmitted after choosing shortest path without any consideration of any particular node's traffic which raises the problem of delay of transmission and unbalanced energy consumption. In this paper ,a new routing metric is introduced to the protocol which selects the lightest loaded path with the largest residual energy for data routing all of the time, thus improve the packet delivery rate and lower the average delay. A comprehensive simulation study was conducted to evaluate the performance of the proposed scheme. Performance results also show that the proposed protocol outperforms existing ad hoc routing protocols in terms of packet delivery and average end-to-end delay.
Diagnostic polymerase chain reaction. (1997)

In this overview, the PCR sensitivities and specificities for diagnosis of B. pertussis in seven different pertussis vaccine studies are discussed. The performance sensitivity of the PCR methods ranged from < or = 1 to 10 cfu, and the diagnostic sensitivity from 73 to 100% when culture was used as a reference. The proportional increase in cases by positive PCR among culture-negatives ranged from 71 to 293% (four trials), whereas the gain was 6 to 11% among culture and serology-negatives (two trials). As the triggers for collecting nasopharyngeal samples, the selection of samples for PCR analysis, and the PCR protocols themselves differed between the trials, these figures must be interpreted with caution. However, the overall results indicate a considerable gain in sensitivity when PCR was added to culture, but a moderate gain in addition to serology.
Ka-band RF MEMS phase shifters (1999)

As the need for low-loss phase shifters increases, so does the interest in radio frequency (RF) MEMS as a solution to provide them. In this paper, progress in building low loss Ka-band phase shifters using RF MEMS capacitive switches is demonstrated. Using a switched transmission line 4-bit resonant phase shifter, an average insertion loss of 2.25 dB was obtained with better than 15-dB return loss, a similar 3-bit phase shifter produced an average insertion loss of 1.7 dB with better than 13-dB return loss. Both devices had a phase error of less than 13/spl deg/ in the fundamental states. To our knowledge, these devices represent the lowest loss Ka-band phase shifters reported to date.
<i>APC</i> mutations are sufficient for the growth of early colorectal adenomas (2000)

It is not clear whether APC mutations are sufficient for early colorectal adenomas to grow or whether additional mutations at other loci are required. We previously have screened 210 early colorectal adenomas from familial adenomatous polyposis patients for mutations and allelic loss at APC. Here, we determined whether allelic loss at APC had any effect on the nearby alpha-catenin gene. However, loss on 5q in familial adenomatous polyposis adenomas rarely extended as far as alpha-catenin, and no differences in alpha-catenin protein expression were found in tumors that showed loss encompassing both APC and alpha-catenin. We then screened all 210 tumors for mutations at candidate loci other than APC (K-ras, beta-catenin, and allelic loss at 1p33-p35 and 1p36) and for microsatellite instability (MSI). Each of these loci has been implicated previously in early colorectal tumorigenesis. One tumor harbored a beta-catenin mutation and another MSI, but none showed K-ras mutation or allelic loss at 1p33-p35 or 1p36. These data support the following hypotheses derived from sporadic colorectal tumors: beta-catenin mutations are generally an alternative to mutations at APC, MSI is not usually an early phenomenon in colorectal tumorigenesis, and K-ras mutations are more typical of large- and moderate-sized adenomas. Contrary to some previous reports, chromosome 1p allelic loss is infrequent in very early adenomas. APC mutations are generally sufficient for colorectal tumors to grow to about 1-cm diameter, although chance mutations at other loci can provide these early colorectal adenomas with a selective advantage, and some colorectal tumors may develop along a pathway not involving APC.
Dehydrogenation Reactions of Cyclic C<sub>2</sub>B<sub>2</sub>N<sub>2</sub>H<sub>12</sub> and C<sub>4</sub>BNH<sub>12</sub> Isomers (2010)

The energetics for different dehydrogenation pathways of C(2)B(2)N(2)H(12) and C(4)BNH(12) cycles were calculated at the B3LYP/DGDZVP2 and G3(MP2) levels with additional calculations at the CCSD(T)/complete basis set level. The heats of formation of the different isomers were calculated from the G3(MP2) relative energies and the heats of formation of the most stable isomers of c-C(2)B(2)N(2)H(6), c-C(2)B(2)N(2)H(12), and c-C(4)BNH(12) at the CCSD(T)/CBS including additional corrections together with the previously reported value for c-C(4)BNH(6). Different isomers were analyzed for c-C(2)B(2)N(2)H(x) and c-C(4)BNH(x) (x = 6 and 12), and the most stable cyclic structures were those with C-C-B-N-B-N and C-C-C-C-B-N sequences, respectively. The energetics for the stepwise loss of three H(2) were predicted, and the most feasible thermodynamic pathways were found. Dehydrogenation of the lowest energy c-C(2)B(2)N(2)H(12) isomer (6-H(12)) is almost thermoneutral with DeltaH(3dehydro) = 3.4 kcal/mol at the CCSD(T)/CBS level and -0.6 kcal/mol at the G3(MP2) level at 298 K. Dehydrogenation of the lowest energy c-C(4)BNH(12) isomer (7-H(12)) is endothermic with DeltaH(3dehydro) = 27.9 kcal/mol at the CCSD(T)/CBS level and 23.5 kcal/mol at the G3(MP2) level at 298 K. Dehydrogenation across the B-N bond is more favorable as opposed to dehydrogenation across the B-C, N-C, and C-C bonds. Resonance stabilization energies in relation to that of benzene are reported as are NICS NMR chemical shifts for correlating with the potential aromatic character of the rings.
Quadrupole-octupole coupled states in<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msup><mml:mrow/><mml:mrow><mml:mn>114</mml:mn></mml:mrow></mml:msup></mml:mrow><mml:mi mathvariant="normal">Cd</mml:mi></mml:math> (2003)

The properties of negative-parity states in ${}^{114}\mathrm{Cd}$ have been studied with the ${(n,n}^{\ensuremath{'}}\ensuremath{\gamma})$ reaction. $\ensuremath{\gamma}$-ray angular distributions and excitation functions have been used to characterize the decays of these excited levels, and level lifetimes have been obtained with the Doppler-shift attenuation method. A set of five closely spaced, negative-parity states has been identified as the complete quadrupole-octupole quintuplet. These excited states and the observed transitions from these levels are compared to calculations performed in the framework of the $\mathrm{spdf}$ interacting boson model.
Enhanced Sensitivity Employing Zwitterionic and pI Balancing Dyes (Z-CyDyes) Optimized for 2D-Gel Electrophoresis Based on Side Chain Modifications of CyDye Fluorophores. New Tools For Use in Proteomics and Diagnostics (2013)

The CyDye family of fluorescent dyes is currently the overwhelming choice for applications in proteomic analysis, using two-dimensional difference gel electrophoresis (2D-DIGE). Protein labeling with CyDyes is hampered by protein precipitation and gel smearing when used above minimal labeling. The solubility of labeled protein may be improved by introducing water solubilizing groups on the dye such as cysteic acids. However, addition of a negatively charged functionality will have the undesired effect of shifting the pI in relation to the unlabeled protein. These limitations have been addressed through the synthesis of highly water-soluble and pI balancing zwitterionic CyDye fluorophores (Z-CyDyes). The new dyes feature a cysteic acid motif, a titratable amine functionality and a NHS activated ester group. In side by side 2D-DIGE comparisons of Z-CyDyes and CyDyes, the new dyes significantly enhanced protein spot volume and the number of spots that were detected. Z-CyDyes have the potential to enhance the depth of proteome coverage and provide a general strategy for improving the performance of protein tagging reagents.
Wastewater use in algae production for generation of renewable resources: a review and preliminary results (2013)

Microalgae feedstock production can be integrated with wastewater and industrial sources of carbon dioxide. This study reviews the literature on algae grown on wastewater and includes a preliminary analysis of algal production based on anaerobic digestion sludge centrate from the Howard F. Curren Advanced Wastewater Treatment Plant (HFC AWTP) in Tampa, Florida and secondary effluent from the City of Lakeland wastewater treatment facilities in Lakeland, Florida. It was demonstrated that a mixed culture of wild algae species could successfully be grown on wastewater nutrients and potentially scaled to commercial production. Algae have demonstrated the ability to naturally colonize low-nutrient effluent water in a wetland treatment system utilized by the City of Lakeland. The results from these experiments show that the algae grown in high strength wastewater from the HFC AWTP are light-limited when cultivated indoor since more than 50% of the outdoor illumination is attenuated in the greenhouse.An analysis was performed to determine the mass of algae that can be supported by the wastewater nutrients (mainly nitrogen and phosphorous) available from the two Florida cities. The study was guided by the growth and productivity data obtained for algal growth in the photobioreactors in operation at the University of South Florida. In the analysis, nutrients and light are assumed to be limited, while CO2 is abundantly available. There is some limitation on land, especially since the HFC AWTP is located at the Port of Tampa. The temperature range in Tampa is assumed to be suitable for algal growth year round. Assuming that the numerous technical challenges to achieving commercial-scale algal production can be met, the results presented suggest that an excess of 71 metric tons per hectare per year of algal biomass can be produced. Two energy production options were considered; liquid biofuels from feedstock with high lipid content, and biogas generation from anaerobic digestion of algae biomass. The total potential oil volume was determined to be approximately 337,500 gallons per year, which may result in the annual production of 270,000 gallons of biodiesel when 80% conversion efficiency is assumed. This production level would be able to sustain approximately 450 cars per year on average. Potential biogas production was estimated to be above 415,000 kg/yr, the equivalent of powering close to 500 homes for a year.
The nIpD gene is located in an operon with rpoS on the Escherichia coli chromosome and encodes a novel lipoprotein with a potential function in ceil wall formation (1994)

rpoS is the structural gene for sigma s, which is a second vegetative sigma subunit of RNA polymerase in Escherichia coli and is involved in the expression of many stationary phase-induced genes. Upstream of rpoS is an open reading frame (ORF) whose function and regulation have not been studied. Strong overproduction of its gene product using the IPTG-inducible tac promoter leads to the formation of bulges at the cell septum and the cell poles, and in rapidly growing cells brings about cell lysis, indicating that the gene product has a hydrolytic function in cell wall formation or maintenance. This is corroborated by sequence homology to lysostaphin, a cell wall lytic exoenzyme synthesized by two Staphylococcus strains. Using globomycin, a specific inhibitor of signal peptidase II, we demonstrate that the product of the ORF is a novel lipoprotein (NlpD). Two transcriptional start sites for nlpD have been localized. In contrast to rpoS, nlpD is not induced during entry into stationary phase. Growth-phase-regulated transcription of rpoS is initiated at additional sites within the nlpD ORF, but the nlpD promoters contribute substantially to the basal level of rpoS expression in exponentially growing cells, indicating that nlpD and rpoS form an operon.
Carbon Dioxide Capture Using Ionic Liquid 1-Butyl-3-methylimidazolium Acetate (2010)

Carbon dioxide (CO2) capture using aqueous amine scrubbing is currently considered the most feasible option for separating CO2 from post-combustion flue gas. Using simple absorption and stripping configurations, monoethanolamine has been commercially demonstrated to effectively scrub CO2 from post-combustion flue gas. However, the current capital and operating costs are high and do not meet the target of the Department of Energy to remove 90% of CO2 from post-combustion flue gas with no more than a 35% increase in the cost of electricity. The evaluation of advanced absorbents, adsorbents, and membranes is under way to find the most energy-efficient CO2-capture technology. We have modeled an ionic liquid that can reduce the energy losses by 16% compared to a commercial monoethanolamine process. The choice of the ionic liquid, 1-butyl-3-methylimidazolium acetate, has not been optimized but was chosen based on chemical absorption behavior and the desire to understand performance. Engineering design estimates indicate that the investment for the ionic liquid process will be 11% lower than the amine-based process and provide a 12% reduction in equipment footprint. A parametric study examined four improvements in the ionic liquid technology, which may reduce even further the energy and cost required for CO2 capture.
Retinoic Acid Blocks Adipogenesis by Inhibiting C/EBPβ-Mediated Transcription (1997)

Adipocyte differentiation is thought to involve sequential induction of the transcription factors C/EBPbeta, peroxisome proliferator-activated receptor gamma (PPARgamma), and C/EBPalpha. C/EBPalpha expression is both necessary and sufficient for adipocyte differentiation. Here we report that ectopic expression of either C/EBPalpha or C/EBPbeta induces PPARgamma expression and adipogenesis and that retinoic acid (RA) completely inhibits adipogenesis by either form of C/EBP. In studies of normal preadipocytes, RA does not prevent C/EBPbeta induction but blocks induction of PPARgamma, C/EBPalpha, and adipogenesis. In transient transfection studies, liganded RA receptor (RAR) specifically blocks transcriptional activation by either C/EBPalpha or C/EBPbeta. These results strongly suggest that C/EBPalpha substitutes for C/EBPbeta to induce adipocyte differentiation and that liganded RAR inhibits adipogenesis by blocking C/EBPbeta-mediated induction of downstream genes.
Novel Fluorination Reagent: IF5 / Et3N–3HF (2001)

Abstract IF5 in Et3N–3HF was found to be a stable, non-hazardous, easy to handle, and inexpensive reagent that enables effective and selective fluorination of organic compounds under mild conditions.
The Role of Market Entry Timing in the Evaluation of Manufacturing Technology Innovations (1997)

Application of strategic new technologies is a key requirement for the maintenance of a firm's competitive advantage in most markets. The process by which organizations adopt and apply innovative technology products is the subject of investigation. This study of innovation processes includes both individual champion behavior and organizational adoption behavior.^ In technology markets characterized by rapid evolution in both markets and technology, timing seems likely to be an important consideration in the adoption process. This research investigates the impact of the timing of the decision to acquire a specific high speed machining and laser cutting technology on the propensity to champion the adoption and the impact of timing on the outcome of the adoption decision.^ Timing is defined as the temporal distance between the actual or forecast decision date and the temporal reference point (TRP), the time when the firm's management expects to begin to realize negative outcomes should it fail to adopt the specific technology. On this basis time gains (when the decision pre-dates the TRP) and losses (when the decision post-dates the TRP) can be identified. Along with timing, the model of champion behavior also included measures of perceived value and personal risk. The organization adoption model also included measures for strategic intent, risk and decision complexity.^ While timing was not found to significantly influence champion behavior, it did seem to influence the adoption behavior of the organization. Most adoption decisions were found to occur in a region of time loss. In conformance with prospect theory prediction increasing time losses (decisions which increasing post-date the TRP) result in delayed adoption. The date of a management team's TRP was also found to be related to the date of subsequent adoption decisions.^ These findings have implications for marketing theory, practice and research. The temporal reference point is a potential means of identifying early adopters, key prospects, optimum timing for new product introduction, and candidate R & D projects for acceleration. It also provides a basis for forecasting the development of markets for technology products, and potentially enabling experimental designs in domains unaccustomed to such research, e.g., the diffusion of innovations. ^
Comparative Analysis of Twelve Genomes of Three Novel Group 2c and Group 2d Coronaviruses Reveals Unique Group and Subgroup Features (2006)

Twelve complete genomes of three novel coronaviruses-bat coronavirus HKU4 (bat-CoV HKU4), bat-CoV HKU5 (putative group 2c), and bat-CoV HKU9 (putative group 2d)-were sequenced. Comparative genome analysis showed that the various open reading frames (ORFs) of the genomes of the three coronaviruses had significantly higher amino acid identities to those of other group 2 coronaviruses than group 1 and 3 coronaviruses. Phylogenetic trees constructed using chymotrypsin-like protease, RNA-dependent RNA polymerase, helicase, spike, and nucleocapsid all showed that the group 2a and 2b and putative group 2c and 2d coronaviruses are more closely related to each other than to group 1 and 3 coronaviruses. Unique genomic features distinguishing between these four subgroups, including the number of papain-like proteases, the presence or absence of hemagglutinin esterase, small ORFs between the membrane and nucleocapsid genes and ORFs (NS7a and NS7b), bulged stem-loop and pseudoknot structures downstream of the nucleocapsid gene, transcription regulatory sequence, and ribosomal recognition signal for the envelope gene, were also observed. This is the first time that NS7a and NS7b downstream of the nucleocapsid gene has been found in a group 2 coronavirus. The high Ka/Ks ratio of NS7a and NS7b in bat-CoV HKU9 implies that these two group 2d-specific genes are under high selective pressure and hence are rapidly evolving. The four subgroups of group 2 coronaviruses probably originated from a common ancestor. Further molecular epidemiological studies on coronaviruses in the bats of other countries, as well as in other animals, and complete genome sequencing will shed more light on coronavirus diversity and their evolutionary histories.
Transflective In-Plane Switching Liquid Crystal Display (2007)

A single cell gap, single gamma curve, and low operating voltage transflective liquid crystal display (LCD) using an in-plane switching (IPS) cell is proposed. In the IPS cell, the pixilated transparent indium tin oxide electrodes are on the top substrate and the reflectors are on the bottom substrate. The electro-optic properties of the transflective IPS LCD are calculated using three-dimensional simulation software. By optimizing the reflector width, the voltage-dependent transmittance and reflectance curves can be matched
Virus-Induced Maturation and Activation of Autoreactive Memory B Cells (2000)

We have examined B cell populations that participate in distinct phases of the immune response to the influenza virus A/PR/8/34 hemagglutinin (HA) for their susceptibility to negative selection in mice that express the HA as a neo-self-antigen (HA104 mice). We demonstrated previously that specificity for the neo-self-HA causes a population of immunoglobulin G antibody-secreting cells, which dominate the primary response to virus immunization in BALB/c mice, to be negatively selected in HA104 mice. We find here that in contrast to these primary response B cells, HA-specific memory response B cells developed equivalently in HA104 and nontransgenic (BALB/c) mice. Indeed, there was no indication that HA-specific B cells were negatively selected during memory formation in influenza virus-immunized HA104 mice, even though the neo-self-HA can be recognized by memory B cells. Furthermore, HA-specific autoantibodies were induced in the absence of virus immunization by mating HA104 mice with mice transgenic for a CD4(+) HA-specific T cell receptor. These findings indicate that specificity for a self-antigen does not prevent the maturation of autoreactive B cells in the germinal center pathway. Rather, the availability of CD4(+) T cell help may play a crucial role in regulating autoantibody responses to the HA in HA104 mice.
Induction of Therapeutically Relevant Cytotoxic T Lymphocytes in Humans by Percutaneous Peptide Immunization (2006)

Percutaneous peptide immunization (PPI) is a simple and noninvasive immunization approach to induce potent CTL responses by peptide delivery via skin with the stratum corneum removed. After such a barrier disruption in human skin, epidermal Langerhans cells, although functionally matured through the up-regulation of HLA expression and costimulatory molecules, were found to emigrate with a reduced number of dendrites. CD8(+) populations binding to MHC-peptide tetramers/pentamers and producing IFN-gamma appeared in the blood after PPI with HLA class I-restricted antigenic peptides. PPI with melanoma-associated peptides reduced the lesion size and suppressed further development of tumors in four of seven patients with advanced melanoma. These beneficial effects were accompanied by the generation of circulating CTLs with in vitro cytolytic activity and extensive infiltration of tetramer/pentamer-binding cells into regressing lesions. PPI elicited neither local nor systemic toxicity or autoimmunity, except for vitiligo, in patients with melanoma. Therefore, PPI represents a novel therapeutic intervention for cancer in the clinical setting.