gebhart/patrepeval huggingface dataset:
The SCID/Beige mouse as a model to investigate protection against<i>Yersinia pestis</i> (1999)

In this study, we have shown that severe combined immunodeficient/beige mice reconstituted with hyperimmune Balb/c lymphocytes can be used as a model to demonstrate adoptive and passive protection against plague infection. Reconstitution of severe combined immunodeficient/beige mice was successful in nine out of ten mice as demonstrated by spleen colonisation and sustained circulating immunoglobulin titres. Furthermore, an increase in antibody titre was evident after a booster immunisation of reconstituted mice. Presence of circulating antibody correlated with protection against a systemic plague challenge and indicated that in reconstituted mice adoptive transfer of a functional immune system had occurred. The severe combined immunodeficient/beige mouse was also used to demonstrate passive protection against inhaled and systemic plague infection. The reconstituted severe combined immunodeficient/beige mouse model demonstrating protective immunity against plague will be further developed to identify the immune cell subsets responsible for this protection.
Calcium/calmodulin‐dependent protein kinase II activity is increased in sarcoplasmic reticulum from coronary artery ligated rabbit hearts (1999)

A protein kinase activity intrinsic to the sarcoplasmic reticulum was studied in normal and hypertrophied rabbit hearts. The relationship between this kinase activity and phospholamban phosphorylation was examined. Calmodulin-dependent kinase II activity was found to be increased in sarcoplasmic reticulum preparations from hypertrophied hearts compared with normal. This was evident by measuring the phosphotransferase activity of the kinase and also by examining phospholamban phosphorylation by electrophoretic band shift analysis. Increased phospholamban phosphorylation by Calmodulin-dependent protein kinase II was dependent on prior phosphorylation by cAMP-dependent protein kinase, indicating potential crosstalk. Specific immunoblot analysis of the rabbit sarcoplasmic reticulum identified the presence of the delta form of calmodulin dependent protein kinase II and showed it to be up-regulated in hypertrophied hearts.
TOL plasmid in Pseudomonas aeruginosa PAO: thermosensitivity of self-maintenance and inhibition of host cell growth (1978)

The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction and modification abilities were lost by mutations or by growing at high temperature. In addition, the transmissibility of the TOL plasmid from strain PAO to P. putida was low when the plasmid was modified by the donor. By using P. aeruginosa PAO carrying the TOL plasmid, the stability and genetic expression of the plasmid as well as its effect on the host cell growth were examined. Thus the self-maintenance of the plasmid was found to be thermosensitive. Furthermore, the TOL plasmid inhibited the growth of strain PAO at high temperature, accompanied by the formation of some filamentous cells. These thermosensitive properties of the TOL plasmid were host dependent and not exhibited in another strain of P. aeruginosa.
The Immune-Adherence Phenomenon (1953)

NEXT ARTICLEErratum: National Academy of Sciences: Abstracts of Papers Presented at the Autumn Meeting, November 9-11, 1953 Massachusetts Institute of Technology, Cambridge, Massachusetts
Characterization of the effects of different retinoids on the growth and differentiation of a human melanoma cell line and selected subclones. (1980)

The effect of four different retinoids [retinol, 13-cis-retinoic acid (Ro4-3780), beta-all-trans-retinoic acid, and aromatic retinoic acid ethyl ester (Ro10-9359)] on the cellular proliferation (cell number) and biochemical differentiation (tyrosinase activity) of a human melanoma cell line (MIRW) and three subclones was assessed. All four retinoids (10(-6) M) inhibited the cellular proliferation (36 to 42%) and stimulated tyrosinase activity (58 to 72%) in the parent cell line to a similar extent. In contrast, the effects of the different retinoids on three derived melanoma clones was dissimilar. For example, in clone A6, beta-all-transretinoic acid stimulated tyrosinase activity by 48% but caused only a 7% inhibition of cellular proliferation. This retinoid caused a more pronounced effect in the other two subclones, stimulating tyrosinase from 135 to 195% and inhibiting growth 19 to 33%. Al three melanoma clones demonstrated increased tyrosinase activity (110 to 225%) and reduced proliferation (37 to 52%) following exposure to 13-cis-retinoic acid. This retinoid was found overall to be the most effective stimulator of tyrosinase, while retinol was observed to be the least active, stimulating enzyme activity slightly (25%) in only one of three clones. Retinol inhibited proliferation 27 to 33% in two of three melanoma subclones. The aromatic retinoic acid ethyl ester elevated tyrosinase levels in two clones but inhibited the enzyme in one melanoma line. Cellular proliferation, however, was reduced in all three clones. These results suggest that retinoid-induced changes in human melanoma cell growth and differentiation reflect underlying cellular differences and diverse biochemical interactions.
Decline in ribosomal fidelity contributes to the accumulation and stabilization of the master stress response regulator σ<sup>S</sup> upon carbon starvation (2007)

The sigma(S) subunit of RNA polymerase is a master regulator of Escherichia coli that retards cellular senescence and bestows cells with general stress protective functions during growth arrest. We show that mutations and drugs triggering translational errors elevate sigma(S) levels and stability. Furthermore, mutations enhancing translational fidelity attenuate induction of the rpoS regulon and prevent stabilization of sigma(S) upon carbon starvation. Destabilization of sigma(S) by increased proofreading requires the presence of the sigma(S) recognition factor SprE (RssB) and the ClpXP protease. The data further suggest that sigma(S) becomes stabilized upon starvation as a result of ClpP sequestration and this sequestration is enhanced by oxidative modifications of aberrant proteins produced by erroneous translation. ClpP overproduction counteracted starvation-induced stabilization of sigma(S), whereas overproduction of a ClpXP substrate (ssrA-tagged GFP) stabilized sigma(S) in exponentially growing cells. We present a model for the sequence of events leading to the accumulation and activation of sigma(S) upon carbon starvation, which are linked to alterations in both ribosomal fidelity and efficiency.
WebProphet: automating performance prediction for web services (2010)

Today, large-scale web services run on complex systems, spanning multiple data centers and content distribution networks, with performance depending on diverse factors in end systems, networks, and infrastructure servers. Web service providers have many options for improving service performance, varying greatly in feasibility, cost and benefit, but have few tools to predict the impact of these options.A key challenge is to precisely capture web object dependencies, as these are essential for predicting performance in an accurate and scalable manner. In this paper, we introduce WebProphet, a system that automates performance prediction for web services. WebProphet employs a novel technique based on timing perturbation to extract web object dependencies, and then uses these dependencies to predict the performance impact of changes to the handling of the objects. We have built, deployed, and evaluated the accuracy and efficiency of WebProphet. Applying WebProphet to the Search and Maps services of Google and Yahoo, we find WebProphet predicts the median and 95th percentiles of the page load time distribution with an error rate smaller than 16% in most cases. Using Yahoo Maps as an example, we find that WebProphet reduces the problem of performance optimization to a small number of web objects whose optimization would reduce the page load time by nearly 40%.
Forschungsaufgaben (1973)

Die hier vorgelegten Materialien wollen — bei der ungeheuren Fülle der Primär- und Sekundärliteratur — nur als Bausteine für weitere Forschungen auf diesem Gebiet verstanden sein. Noch fehlen einschlägige Untersuchungen zu den petrarkistischen Motiven, so wie Grosse (Naturanrufung) oder Ogle (The White Hand) sie vorgelegt haben, aber auch stilistische Studien vom Range derjenigen D. Alonsos (Korrelation). Die scheinbar originalen Liebesgedichte des 16. und 17. Jhs. sind vielfach noch auf ihre möglichen Quellen (Petrarca oder Petrarkisten, antike oder neulat. Vorlagen) zu überprüfen. Um Fehlurteile über die Lyrik seit der Goethezeit zu vermeiden, verdient auch die sog. Bekenntnisdichtung mit entsprechender Umsicht beurteilt zu werden (vgl. Lenz, »Petrarch«; dazu Livescu, S. 151). Motivanalyse und Quellenforschung dürfen freilich nicht bei der statistischen Aufreihung oder dem historischen Nachweis stehenbleiben, sondern müssen nach dem Verhältnis von Tradition und Umbildung, nach dem Ausdruckswert und der künstlerischen Absicht fragen, um die Eigenart eines Werkes auf dem Hintergrund der Überlieferung bestimmen zu können. Eine komparatistische Perspektive ist daher unerläßlich, weil das Imitationsprinzip gerade während der jahrhundertelangen Blüte des Petrarkismus keinesfalls vor Landes- und Sprachgrenzen haltmachte.
An acoustic-phonetic-based speaker adaptation technique for improving speaker-independent continuous speech recognition (1994)

A new speaker adaptation technique is proposed for improving speaker-independent continuous speech recognition based on a decomposition of spectral variation sources. In this technique, the spectral variations are separated into two categories, one acoustic and the other phone-specific, where each variation source is modeled by a linear transformation system. The technique consists of two sequential steps: first, acoustic normalization is performed, and second, phone model parameters are adapted. Experiments of speaker adaptation on the TIMIT database using short calibration speech (5 s per speaker) have shown significant performance improvement over the baseline speaker-independent continuous speech recognition, where the recognition system uses Gaussian mixture density based hidden Markov models of phone units. For a vocabulary size of 853 and test set perplexity of 104, the recognition word accuracy has been improved from 86.9% for the baseline system to 90.5% after adaptation, corresponding to an error reduction of 27.5%. On a more difficult test set that contains an additional variation source due to recording channel mismatch, a more significant performance improvement has been obtained: for the same vocabulary and a test set perplexity of 101, the recognition word accuracy has been improved from 65.4% for the baseline to 86.0% after adaptation, corresponding to an error reduction of 59.5%.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Carbon-fiber ultramicroelectrodes modified with conductive polymeric tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin for determination of nickel in single biological cells (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-fiber ultramicroelectrodes modified with conductive polymeric tetrakis(3-methoxy-4-hydroxyphenyl)porphyrin for determination of nickel in single biological cellsFrederick. Bailey, Tadeusz. Malinski, and Frederick. KiechleCite this: Anal. Chem. 1991, 63, 4, 395–398Publication Date (Print):February 15, 1991Publication History Published online1 May 2002Published inissue 15 February 1991https://pubs.acs.org/doi/10.1021/ac00004a018https://doi.org/10.1021/ac00004a018research-articleACS PublicationsRequest reuse permissionsArticle Views129Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improving the electrochemical properties of Al, Zr Co-doped Li4Ti5O12 as a lithium-ion battery anode material (2014)

Li4Ti5O12 and Al3+, Zr4+ co-doped Li(4−x/3)Al x Ti(5−5x/3)Zr x O12 (x = 0.01, 0.05, 0.1, 0.15, 0.2) were synthesized at 950 °C via a solid state reaction by using rutile TiO2, Li2 CO3, and Al2O3 as precursors for the anode material of a lithium-ion battery. The average particle sizes of Li(4−x/3)Al x Ti(5−5x/3)Zr x O12 (x = 0, 0.01, 0.05, 0.1, 0.15, 0.2) range from 700 to 1200 nm. The particle sizes of pure Li4Ti5O12 and Al3+, Zr4+ co-doped Li4Ti5O12 were not obviously different, but did result in a shift in the (111) peak in X-ray diffraction. Li(4−x/3)Al x Ti(5−5x/3)Zr x O12 (x = 0.01) exhibits an excellent rate capability with a reversible capacity of 127.7 mAh/g at a 5 C-rate and even 113.1 mAh/g at a 10 C-rate. The capacity retention was improved remarkably compared to that for an undoped anode when discharged at a high C- rate.
Space Charge Instabilities in the rf Induced Gas Discharge of the Diode Configuration (1970)

An instability characteristic of resonance sustained plasmas has been observed in the rf planar diode gas discharge at low pressures (2 to 5×10−3 Torr) and small electrode spacings (less than about 2.5 cm). The gross form of this instability may be observed as a breakup of the discharge into spoke shaped plasmoids which may or may not be rotating. An additional mode consisting of a torus shaped discharge near the target edge superimposed on a weaker uniform glow has also been detected. Enhanced radiation of rf power is found to be associated with the breakup phenomenon. One result of these instabilities is that the ion current density incident upon the cathode (the target electrode during sputtering) is nonuniform, usually reaching a maximum near the periphery. Under such conditions, it is shown that the thickness uniformity of a thin film may be either significantly improved or severely degraded with respect to what might be expected assuming a constant ion current density over the target surface and a cosine distribution for the sputtered material. On the other hand, for sputter etching, the etch rate is directly proportional to the ion current density, and hence is generally nonuniform.
<title>Mechanical properties of SiB<formula><inf><roman>6</roman></inf></formula> addition of carbon sintered body</title> (2001)

Boride material is said as the useful material, which has high melting point and high strength. B<SUB>4</SUB>C in carbide is very hard at the next of the diamond and cubic-BN in the Mohs hardness and B<SUB>4</SUB>C has excellent chemical stability and high strength. B<SUB>4</SUB>C is being used as the polishing material from the hardness. However, it is difficult to make sintered body from high melting point (2623 K). Several silicon boride phases such as SiB<SUB>4</SUB>, SiB<SUB>6</SUB>, SiB<SUB>6-x</SUB>, SiB<SUB>6+x</SUB>, and Si<SUB>11</SUB>B<SUB>31</SUB>, were previously reported. Silicon hexaboride (SiB<SUB>6</SUB>) has proved to be a potentially useful material because of its high degree of hardness, moderate melting point (2123 K), and low specific gravity. We studied the preparation of SiB<SUB>6</SUB>-B<SUB>4</SUB>C-SiC sintered body in this report. We knew experientially that SiB<SUB>6</SUB> reacts with carbon at the high temperature, and forms B<SUB>4</SUB>C or SiC. Carbon addition SiB<SUB>6</SUB> sintered body produced by hot pressing and reaction sintering that sintering condition was 1973 K for 3.6 ks in vacuum under a pressure of 25 MPa. The relative density of sintered bodies (SiB<SUB>6</SUB>-0,5,10,15 wt%C) was approximately 100%. Characterization of mechanical properties was used indentation, Vickers hardness and thermal
Programa experimental para fortalecer las habilidades científicas en estudiantes de tres años de una Institución Educativa de Huancavelica (2020)

La presente investigacion tuvo como objetivo determinar la influencia del programa experimental para fortalecer las habilidades cientificas de los estudiantes de 3 anos de una Institucion Educativa de Huancavelica, para ello se empleo el metodo cientifico como general y especifico el metodo experimental, por ser de tipo aplicada con un diseno pre experimental porque se manipulo la variable independiente, la muestra estuvo conformada por 25 estudiantes. Entre las conclusiones obtenidas, fueron: el programa experimental permitio fortalecer las habilidades cientificas en los estudiantes de 3 anos, donde segun el test de entrada, 22 de ellos que hacen el 88% expresaron habilidades cientificas en un nivel de bajo y 3 que representan al 12% muestran habilidades cientificas en el nivel medio; mientras que el post test se evidencio que 3 que hacen el 12% tienen habilidades cientificas en un nivel medio y 22 que representan al 88% de los estudiantes tienen habilidades cientificas en el nivel alto.
A Perfluoroaryl-Cysteine S <sub>N</sub> Ar Chemistry Approach to Unprotected Peptide Stapling (2013)

We report the discovery of a facile transformation between perfluoroaromatic molecules and a cysteine thiolate, which is arylated at room temperature. This new approach enabled us to selectively modify cysteine residues in unprotected peptides, providing access to variants containing rigid perfluoroaromatic staples. This stapling modification performed on a peptide sequence designed to bind the C-terminal domain of an HIV-1 capsid assembly polyprotein (C-CA) showed enhancement in binding, cell permeability, and proteolytic stability properties, as compared to the unstapled analog. Importantly, chemical stability of the formed staples allowed us to use this motif in the native chemical ligation-mediated synthesis of a small protein affibody that is capable of binding the human epidermal growth factor 2 receptor.
Complex Haar transform (1976)

A new discrete orthogonal transform called complex Haar transform (CHT) is defined and developed. Several of its properties, such as linearity, uniqueness, dyadic autocorrelation, and dyadic shift-invariant spectrum, are outlined. Efficient algorithms for fast computation of CHT and its inverse are developed. The effectiveness of CHT in signal and image processing applications is investigated.
PKCalpha/beta I inhibitor Go6976 induces dephosphorylation of constitutively hyperphosphorylated Rb and G1 arrest in T24 cells. (2010)

The results show that Go6976 can be used to restore constantly hyperphosphorylated and therefore constantly inactive Rb function in cancer cells.
A Novel Therapy to Attenuate Acute Kidney Injury and Ischemic Allograft Damage after Allogenic Kidney Transplantation in Mice (2015)

Ischemia followed by reperfusion contributes to the initial damage to allografts after kidney transplantation (ktx). In this study we tested the hypothesis that a tetrapeptide EA-230 (AQGV), might improve survival and attenuate loss of kidney function in a mouse model of renal ischemia/reperfusion injury (IRI) and ischemia-induced delayed graft function after allogenic kidney transplantation. IRI was induced in male C57Bl/6N mice by transient bilateral renal pedicle clamping for 35 min. Treatment with EA-230 (20-50mg/kg twice daily i.p. for four consecutive days) was initiated 24 hours after IRI when acute kidney injury (AKI) was already established. The treatment resulted in markedly improved survival in a dose dependent manner. Acute tubular injury two days after IRI was diminished and tubular epithelial cell proliferation was significantly enhanced by EA-230 treatment. Furthermore, CTGF up-regulation, a marker of post-ischemic fibrosis, at four weeks after IRI was significantly less in EA-230 treated renal tissue. To learn more about these effects, we measured renal blood flow (RBF) and glomerular filtration rate (GFR) at 28 hours after IRI. EA-230 improved both GFR and RBF significantly. Next, EA-230 treatment was tested in a model of ischemia-induced delayed graft function after allogenic kidney transplantation. The recipients were treated with EA-230 (50 mg/kg) twice daily i.p. which improved renal function and allograft survival by attenuating ischemic allograft damage. In conclusion, EA-230 is a novel and promising therapeutic agent for treating acute kidney injury and preventing IRI-induced post-transplant ischemic allograft injury. Its beneficial effect is associated with improved renal perfusion after IRI and enhanced regeneration of tubular epithelial cells.
Peptide with Angiotensin I-Converting Enzyme Inhibitory Activity from Hydrolyzed Corn Gluten Meal (2007)

Corn gluten meal (CGM) was hydrolyzed by Alcalase after starch removal of CGM was applied as a pretreatment. A new inhibitory peptide for angiotensin I-converting enzyme (ACE) was isolated from the hydrolysate of CGM with the use of Bio-Rad P-2 gel filtration and followed by reverse-phase high-performance liquid chromatography (RP-HPLC). The sequence of the active peptide was determined to be Ala-Tyr after the application of amino acid analysis and HPLC/MS. The IC50 of the peptide was 14.2 microM, and it was not affected by preincubation with 30 mU of ACE at 37 degrees C for 3 h. Ala-Tyr also exerted antihypertensive effects after oral administration to spontaneously hypertensive rats. A maximal reduction of systolic blood pressure of 9.5 mmHg was observed 2 h after oral administration of Ala-Tyr at doses of 50 mg/kg.
Synthesis and aging effect of spherical magnetite (Fe3O4) nanoparticles for biosensor applications (2003)

The chemical coprecipitation process was used to synthesize about 7 nm, spherical magnetite nanoparticles to study magnetic properties and the aging effect. As-produced spherical magnetite nanoparticles have been aged in the atmosphere for 19 months. Magnetic properties and aging effect were studied by Mössbauer spectroscopy at a temperature ranging from 77 to 300 K, vibrating sample magnetometer, and x-ray diffraction. Saturation magnetization and coercivity were found to be 49 emu/g and nearly 0 Oe at room temperature, respectively. A singlet Mössbauer spectrum was observed at room temperature, implying superparamagnetic behavior of the particles, while a two-sextet spectrum was observed at 77 K. The particle size in this study is about 7 nm, which is smaller than the superparamagnetic size of 26 nm as calculated from Neel’s theory of single domain particles. After having aged these particles for 19 months, all magnetic properties and their original shapes were retained. Superparmagnetic magnetite nanoparticles synthesized in this study can be applied to microbead applications of a biosensor.