gebhart/patrepeval huggingface dataset:
Transplantation: Posttransplant Lymphoproliferative Disorder (2007)

Screening for patients at risk and balancing the intensity of immunosuppressive regimens against the risk of rejection can substantially reduce the risk of developing PTLD. If PTLD occurs, an individualized treatment plan including decreased immunosuppression and other agents should be chosen based on the severity and extent of disease.
Directed sputter deposition of AlCu: Film microstructure and microchemistry (1994)

The microstructure and microchemistry of AlCu films deposited onto unheated oxidized Si(001) substrates by two recently developed directional sputtering techniques, collimated magnetron sputter deposition (CMSD) and ionized magnetron sputter deposition (IMSD), have been investigated using Rutherford backscattering spectroscopy, x-ray diffraction (XRD), plan-view transmission electron microscopy (TEM), cross-sectional TEM (XTEM), and scanning TEM (STEM). Both sets of films had essentially the same average compositions as the alloy targets while exhibiting dense columnar microstructures with (111) preferred orientation, relatively abrupt film/ substrate interfaces, and no indication of surface faceting. Microstructure evolution pathways, however, were quite different. IMSD layers exhibited competitive column growth resulting in a degree of (111) orientation which was more than an order of magnitude larger than that found in corresponding CMSD films and, for thicker layers (≳1 μm), larger average column sizes. The IMSD films also had a much smaller spread in the azimuthal distribution of (111)-oriented columns. XRD line broadening analyses and TEM/XTEM examinations revealed that IMSD layers contained inhomogeneous strain due to residual ion-irradiation-induced defects and, in the upper near-surface region, Al2Cu precipitates while CMSD layers appeared defect-free in TEM/XTEM images. The IMSD films were slightly tensile (+0.13±0.01 GPa) and the CMSD films were slightly compressive (−0.18±0.06 GPa).
Measuring nonhomologous end-joining, homologous recombination and alternative end-joining simultaneously at an endogenous locus in any transfectable human cell (2021)

Double strand break (DSB) repair primarily occurs through 3 pathways: non-homologous end-joining (NHEJ), alternative end-joining (Alt-EJ), and homologous recombination (HR). Typical methods to measure pathway usage include integrated cassette reporter assays or visualization of DNA damage induced nuclear foci. It is now well understood that repair of Cas9-induced breaks also involves NHEJ, Alt-EJ, and HR pathways, providing a new format to measure pathway usage. Here, we have developed a simple Cas9-based system with validated repair outcomes that accurately represent each pathway and then converted it to a droplet digital PCR (ddPCR) readout, thus obviating the need for Next Generation Sequencing and bioinformatic analysis with the goal to make Cas9-based system accessible to more laboratories. The assay system has reproduced several important insights. First, absence of the key Alt-EJ factor Pol θ only abrogates ∼50% of total Alt-EJ. Second, single-strand templated repair (SSTR) requires BRCA1 and MRE11 activity, but not BRCA2, establishing that SSTR commonly used in genome editing is not conventional HR. Third, BRCA1 promotes Alt-EJ usage at two-ended DSBs in contrast to BRCA2. This assay can be used in any system, which permits Cas9 delivery and, importantly, allows rapid genotype-to-phenotype correlation in isogenic cell line pairs.
Design and performance of Weft-Knitting Machinery (1968)

In recent years the knitting industry has expanded\nconsiderably due to the increased demand for knitted fabrics. Not only does the weft knitting industry produce more of the fabrics which are exclusive, to the kntting trade but it has also branched out into fields which were previously only open to the weaving trade.\n\nThis increasing demand is partly due to the development\nof new fibres,but mainly to the technological improvements of the knitting machines,which now provide many new structures and more scope for patterned-fabrics.\nAnother improvement in weft knitting machinery has been an increased rate of fabric production, but in this field the limitations of the machines have now almost been reached.\n\nThe work discussed in the following chapters is primarily\nconcerned with an investigation into the fundamentals of the weft knitting machine to see if substantial increases in rates of fabric production can be achieved.
Analyzing multiple configurations of a C program (2005)

Preprocessor conditionals are heavily used in C programs since they allow the source code to be configured for different platforms or capabilities. However, preprocessor conditionals, as well as other preprocessor directives, are not part of the C language. They need to be evaluated and removed, and so a single configuration selected, before parsing can take place. Most analysis and program understanding tools run on this preprocessed version of the code so their results are based on a single configuration. This paper describes the approach of CRefactory, a refactoring tool for C programs. A refactoring tool cannot consider only a single configuration: changing the code for one configuration may break the rest of the code. CRefactory analyses the program for all possible configurations simultaneously. CRefactory also preserves preprocessor directives and integrates them in the internal representations. The paper also presents metrics from two case studies to show that CRefactory's program representation is practical.
High-efficiency fracturing fluids for low-temperature reservoirs (1985)

The use of various metal salts and metal chelates as crosslinking materials for guar gum and guar gum derivatives has been investigated under low temperature conditions (less than or equal to200/sup 0/F). To determine the cross-linkers of highest efficiency, these crosslinkers were evaluated using the criteria of maximum crosslinked viscosity yield per pound of polymer employed, relative shear sensitivity of each crosslinker and projected ease of application for each crosslinked fluid system in the field. Through optimization of crosslinker-polymer interaction involving development of a delayed crosslinker for low temperature conditions, improved fracturing fluids have been developed. The polymer loadings in these systems can be dropped up to 50% without losing fluid efficiency. The performance of the crosslinked fluid system which showed the highest efficiency was taken to the field and its performance evaluated on a number of wells and compared to other conventional systems.
Recognition of a Ubiquitous Self Antigen by Prostate Cancer-Infiltrating CD8 <sup>+</sup> T Lymphocytes (2008)

Substantial evidence exists that many tumors can be specifically recognized by CD8+ T lymphocytes. The definition of antigens targeted by these cells is paramount for the development of effective immunotherapeutic strategies for treating human cancers. In a screen for endogenous tumor-associated T cell responses in a primary mouse model of prostatic adenocarcinoma, we identified a naturally arising CD8+ T cell response that is reactive to a peptide derived from histone H4. Despite the ubiquitous nature of histones, T cell recognition of histone H4 peptide was specifically associated with the presence of prostate cancer in these mice. Thus, the repertoire of antigens recognized by tumor-infiltrating T cells is broader than previously thought and includes peptides derived from ubiquitous self antigens that are normally sequestered from immune detection.
Highly Fluorescent BF<sub>2</sub> Complexes of Hydrazine–Schiff Base Linked Bispyrrole (2014)

A series of BF2 complexes of hydrazine-Schiff base linked bispyrrole have been prepared from a simple two-step reaction from commercially available substances and are highly fluorescent in solution, film, and solid states with larger Stokes shift and excellent photostabilities comparable or even super to those of their BODIPY analogues. These resultant fluorescent dyes are highly susceptible to the postfunctionalization, as demonstrated in this work via the Knoevenagel condensation to introducing functionalities or tether groups to the chromophore.
Filters for the Infrared (1936)

A simple filtering system, involving the use of only two simply constructed selenium powder filters, is described, by the use of which it is possible to obtain a pure energy spectrum with a wire grating spectrometer in the region 40–130μ. In addition transmission curves are given for a number of substances in the regions 1–15μ and 35–130μ.
Super Stretched Solder Interconnects for Wafer Level Packaging (2006)

A cost-effective wafer level packaging technique termed "stretch and break", based on stretching and detachment of solder interconnections, has been established. Excellent co-planarity, essential for wafer level test and burn-in, is inherent in the process. The technique allows the freedom to use solder materials of up to 400degC melting temperature for forming the interconnection. The shape of the interconnection can also be easily manipulated for optimum performance. The mechanical and thermal cycling reliabilities of the stretched solder interconnection have been found to be significantly better than those of conventional solder joints
Analysis of dominant lactic acid bacteria from artisanal raw milk cheeses produced on the mountain Stara Planina, Serbia (2011)

Traditional Serbian cheese production has a long history and generates products with rich flavor profiles. To enable the industrial manufacture of these home-made Serbian cheeses, the lactic acid bacteria present in them needs to be characterized. Five fresh white cheeses made from raw cow?s milk without commercial starter cultures were collected from households on the mountain Stara Planina, Serbia. According to phenotypical and molecular analysis, 262 isolated Lwere found to belong to Lactococcus, Lactobacillus, Streptococcus, Leuconostoc or Enterococcus. The unique bacterial composition of each cheese indicates that the preservation of household industry is the way to maintain production of distinct cheeses.
Rare earth oxide catalyzed oxidation of rhenium to rhenate ions ReO4- and ReO3- as observed by negative surface ionization mass spectrometry (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRare earth oxide catalyzed oxidation of rhenium to rhenate ions ReO4- and ReO3- as observed by negative surface ionization mass spectrometryJ. E. DelmoreCite this: J. Phys. Chem. 1987, 91, 11, 2883–2886Publication Date (Print):May 1, 1987Publication History Published online1 May 2002Published inissue 1 May 1987https://pubs.acs.org/doi/10.1021/j100295a044https://doi.org/10.1021/j100295a044research-articleACS PublicationsRequest reuse permissionsArticle Views115Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Correlation of Amine Number and pDNA Binding Mechanism for Trehalose-Based Polycations (2008)

Glycopolymers with repeat units comprised of the disaccharide trehalose and an oligoamine of increasing amine have been previously synthesized by our group and shown to efficiently deliver pDNA (plasmid DNA) to HeLa cells while remaining relatively nontoxic. Complexes formed between the most amine-dense of these polycations and pDNA were also found to be relatively stable in serum and have low aggregation, which is desirable for in vivo gene delivery. To lend insight into these interesting results, this study was aimed at investigating the binding strength and mechanism of interaction between these macromolecules, via isothermal titration calorimetry (ITC) and ethidium bromide exclusion assays. The size of these pDNA-polymer complexes, or polyplexes, at various states of formation was determined through light scattering and zeta-potential measurements. Varying degrees of pDNA secondary structure change occurred upon interaction with the polymers, as evidenced by circular dichroism spectra through increasing molar ratios of polymer amine to DNA phosphate, and Fourier transform infrared (FT-IR) results demonstrated stronger electrostatic binding with the phosphate backbone with the least amine-dense of the series. It was concluded that, depending on the number of secondary amines in the repeat unit, these polymers interact with pDNA via different mechanisms with varying extents of electrostatic interaction and hydrogen bonding. These differing mechanisms may affect the ability of trehalose to serve as a deterrent against aggregation in serum conditions and lend insight into the roles of polymer-pDNA binding during the complex transfection process.
PRELIMINARY CHARACTERIZATION OF THE 5α-DIHYDROTESTOSTERONE BINDING PROTEIN IN THE EPIDIDYMAL CYTOSOL FRACTION. IN VIVO STUDIES (1972)

ABSTRACT Following the administration of [ 3 H] testosterone to castrated rats in vivo, most of the radioactivity in the epididymal cytosol fraction was bound to macromolecules. The molecular weight of the androgen-macromolecular complexes was determined to about 90 000, and the frictional ratio (f/fo) was 1.62. The sedimentation coefficient was shown to be 4.6 S (± 0.2) and the complex was slightly retarded on a column of Sephadex G-100 (Einstein-Stokes radius 47–48 Å). On polyacrylamide gel electrophoresis it moved as a sharp zone with R F : 0.41. Almost all the radioactivity bound to these macromolecules was identified as 5α-dihydrotestosterone (5α-DHT) (95%) and only a minor part (3–4%) as testosterone. The receptor for 5α-DHT in the epididymal cytosol fraction was in its physico-chemical properties shown to be different from the androgenbinding proteins of the rat ventral prostate, and could not be demonstrated in non-target organs like liver and kidney. The receptor for 5α-DHT in the epididymal cytosol fraction did not form aggregates in hypotonic solutions, and was roughly of the same molecular size both at high and low salt concentrations.
Reservoir Stochastic Modeling Constrained by Quantitative Geological Conceptual Patterns (2006)

这份报纸在水库上讨论地质的限制的原则随机的建模。由使用系统科学理论，包括随机的无常和模糊无常，二种无常被认出。以便改进随机的建模的精确并且在实现减少无常，模糊无常应该被强调，并且“地质的控制开始的建模”在一个量的地质的模式的指导下面被进行。在 Ordos 盆的 Ansai 油矿的 Pingqiao 水平井的部门的一个例子被举详细说明随机的建模的方法。
Biosurfaces: A Materials Science and Engineering Perspective (2015)

"Ideal as a graduate textbook, this book is aimed at helping design effective biomaterials, taking into account the complex interactions that occur at the interface when a synthetic material is inserted into a living system. Surface reactivity, biochemistry, substrates, cleaning, preparation, and coatings are presented, with numerous case studies and applications throughout. Highlights include: Starts with concepts and works up to real-life applciations such as implantable devices, medical devices, prosthetics, and drug delivery technology Addresses surface reactivity, requirements for surface coating, cleaning and preparation techniques, and characterization Discusses the biological response to coatings Addresses biomaterial-tissue interaction Incorporates nanomechanical properties and processing strategies"--Provided by publisher.;"This book is aimed at helping design effective biomaterials, taking into account the complex interactions that occur at the interface when a synthetic material is inserted into a living system. Surface reactivity, biochemistry, substrates, cleaning, preparation, and coatings are presented, with numerous case studies and applications throughout"--Provided by publisher
Development of an AdEasy‐based system to produce first‐ and second‐generation adenoviral vectors with tropism for CAR‐ or CD46‐positive cells (2012)

In the present study, an AdEasy system tailored for the production of not only first-, but also second-generation AdVs equipped with the receptor-interacting fiber domains of the prototypic species C HAdV5 or of the species B member HAdV50 is presented. This system expands the range of applications for this robust and versatile AdV production platform.
Studies on Mast Cells and Histamine Release in Psoriasis: The Effect of Ranitidine (1998)

The purpose of this study was to investigate histamine and skin mast cells in psoriasis before and during 6 months of treatment with high-dose ranitidine. Sixteen psoriasis patients, presenting a mean PASI score of 15.4, were compared with 13 age- and sex-matched healthy controls. Resting extracellular skin levels of histamine and histamine release to mast cell secretagogues, as measured by the microdialysis technique, were increased in involved psoriasis skin compared to normal skin in the controls. Plasma histamine, but not basophil histamine release, was significantly increased in the patients. Mast cells and lymphocytes were significantly increased in numbers in involved versus non-involved skin in the patients, the lymphocytes being predominantly T-lymphocytes expressing HLA-DR activation. During 6 months of ranitidine treatment, mean PASI score of 15.4 decreased to 5.8. The lymphocyte infiltration, but not mast cell numbers, was significantly reduced during treatment, and histamine release to mast cell secretagogues was normalized. These observations suggest that skin mast cells in active psoriasis are functionally hyperreactive. The biochemical findings together with the clinical effect of ranitidine indicate that histamine may be involved in the pathophysiology of psoriasis.
Effect of emitter-base spacing on the current gain of AlGaAs/GaAs heterojunction bipolar transistors (1989)

An investigation of the effect of surface recombination and emitter-base-contact spacing on the DC current-gain of AlGaAs/GaAs heterojunction bipolar transistor (HBT) using thin AlGaAs emitter structures is discussed. The selectively-etched, thin-AlGaAs-emitter layer has been used to prevent an exposed extrinsic base region, which has previously limited current gain because of high surface recombination. It is found that a factor of approximately 50 improvement in the current gain can be achieved by proper surface passivation and emitter-base-contact spacing.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Circular Dichroism Spectra of Some Transition Metal Complexes with (1<i>R</i>,2<i>R</i>)-1,2-Cyclopentanediamine (1979)

Abstract Bischelates, three isomers of dicyanocobalt(III), trans-dichlorocobalt(III), and copper(II), and a monochelate, tetracyanoferrate(II), of the title diamine were prepared, and their CD spectra were reported. Major positive components were found for Ia bands, as was to be expected for the preferred λ conformation, but minor positive components were observed for the Ib band with the trans-dianionocobalt(III) complexes. The configurational and the vicinal effects, as calculated from the CD spectra of the cis-dicyanocobalt(III) complexes, are shown to be different from those of (1R,2R)-1,2-cyclohexanediamine. The CD spectra of the Fe(II) and Cu(II) complexes are consistent with the conformational effect of the title diamine.
Combined evaluation of Rad51 and ERCC1 expressions for sensitivity to platinum agents in non‐small cell lung cancer (2007)

DNA repair enzyme expression in tumor cells possibly affects sensitivity to anti-cancer agents. The aim of this study was to determine the relationship between expression status of DNA repair enzymes and chemosensitivity in patients with non-small cell lung cancer (NSCLC). NSCLC tissues prepared from the surgical specimens of 41 patients were subjected to immunohistochemical analysis for Rad51 and ERCC1 proteins and to a chemosensitivity test using the MTT assay. The relationships between the expression status of the DNA repair enzymes and ex vivo chemosensitivity to various agents were evaluated. A positive expression for Rad51 and ERCC1 was observed in 17 cases (41%) and 20 cases (49%), respectively. The positivity of Rad51 was closely related to a certain histologic type of squamous cell carcinoma and poor differentiation, and the positivity of ERCC1 tended to be related to squamous cell carcinoma. In chemosensitivity tests, sensitivities to CDDP and CBDCA were significantly lower when both 2 enzymes were positive (p = 0.012 and 0.04 in CDDP, 0.014 and 0.03 in CBDCA). Both Rad51 and ERCC1 expressions showed no significant relationship with sensitivities to paclitaxel, etoposide, vinorelbine, gemcitabine, 5-FU, or irinotecan. In conclusion, combined expression of Rad51 and ERCC1 expression is associated with resistance to platinum agents in the ex vivo study of clinical NSCLC, and evaluation of expression status of both DNA repair enzymes would be a predictor for clinical response to platinum-based chemotherapies.
Synthesis, Crystal Structures, and Ion-Exchange Properties of a Novel Porous Titanosilicate (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis, Crystal Structures, and Ion-Exchange Properties of a Novel Porous TitanosilicateDamodara M. Poojary, Roy A. Cahill, and Abraham ClearfieldCite this: Chem. Mater. 1994, 6, 12, 2364–2368Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December 1994https://doi.org/10.1021/cm00048a024RIGHTS & PERMISSIONSArticle Views791Altmetric-Citations154LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (601 KB) Get e-Alertsclose Get e-Alerts