gebhart/patrepeval huggingface dataset:
miR-375 Inhibits Differentiation of Neurites by Lowering HuD Levels (2010)

Neuronal development and plasticity are maintained by tightly regulated gene expression programs. Here, we report that the developmentally regulated microRNA miR-375 affects dendrite formation and maintenance. miR-375 overexpression in mouse hippocampus potently reduced dendrite density. We identified the predominantly neuronal RNA-binding protein HuD as a key effector of miR-375 influence on dendrite maintenance. Heterologous reporter analysis verified that miR-375 repressed HuD expression through a specific, evolutionarily conserved site on the HuD 3' untranslated region. miR-375 overexpression lowered both HuD mRNA stability and translation and recapitulated the effects of HuD silencing, which reduced the levels of target proteins with key functions in neuronal signaling and cytoskeleton organization (N-cadherin, PSD-95, RhoA, NCAM1, and integrin alpha1). Moreover, the increase in neurite outgrowth after brain-derived neurotrophic factor (BDNF) treatment was diminished by miR-375 overexpression; this effect was rescued by reexpression of miR-375-refractory HuD. Our findings indicate that miR-375 modulates neuronal HuD expression and function, in turn affecting dendrite abundance.
P-113 (2012)

Johns Hopkins Bloomberg School of Public Health, USA, [email protected] Johns Hopkins Bloomberg School of Public Health, USA Geisinger Center for Health Research, USA Johns Hopkins Bloomberg School of Public Health, USA
Proline-Catalyzed Sequential <i>syn</i>-Mannich and [4 + 1]-Annulation Cascade Reactions To Form Densely Functionalized Pyrrolidines (2015)

A highly efficient one-pot [4 + 1]-annulation process for the asymmetric synthesis of densely functionalized pyrrolidine derivatives is described. The in situ generated syn-Mannich adduct obtained via proline catalysis acts as a four-atom component, and Corey's sulfur ylide or ethyl bromoacetate acts as a one-atom carbon source to construct pyrrolidine units in a highly enantio- and diastereoselective manner.
CPP32 inhibition prevents Fas‐induced ceramide generation and apoptosis in human cells (1996)

Intracellular activation of sphingomyelinase, leading to ceramide generation, and ICE-like proteases have been implicated in TNF and Fas-induced apoptosis, but the links between these intracellular apoptotic mediators remain undefined. We show here that a specific peptide inhibitor of the ICE-like protease CPP32/Yama (DEVD-CHO) blocks anti-Fas-induced apoptosis in Jurkat and U937 cells, while having no effect on TNF-induced apoptosis in U937 cells. This peptide also prevents ceramide accumulation induced by Fas engagement. Jurkat and U937 cells, as well as their mtDNA-depleted derived lines (rho degree cells), were sensitive to ceramide toxicity, which was not prevented by ICE-like protease inhibitors. These results, taken together, suggest that ICE-like protease activation is a prerequisite for ceramide generation and subsequent apoptosis, at least in the case of Fas-induced cell death.
New paraherquamide antibiotics with anthelmintic activity. (1991)

Paraherquamide (1) an anthelmintic alkaloid, was isolated from a species of Penicillium along with three novel analogues 2, 3 and 4. Anthelmintic activity at least equal to that of paraherquamide is described for the 14-de-hydroxy compound, VM 54159 (4).
Optimal data compression for multisensor target tracking with communication constraints (2004)

Target tracking using multiple sensors is one important application in military surveillance and industrial sensing. Due to the communication constraints between each sensor and the data processor that estimates the target state using all the data from multiple sensors, it is crucial for each sensor to compress its measurements optimally so that the data processor can estimate the target state with minimum mean square error. We limit the data compression at each sensor to be a linear transform that reduces the measurement dimension. We use the results by Zhang et al. (2003) to do measurement compression and obtain the optimal linear transform matrix for each sensor based on steady state analysis. To activate or remove a sensor dynamically, we consider a sequential update scheme to modify the data compression matrix for each sensor with an arbitrary dimensional requirement due to the communication constraint. We compare our approach with traditional centralized and distributed tracking schemes and indicate the advantages of using sensor data compression for tracking in a sensor network environment. Simulation results with three sensors show that the estimation accuracy of the proposed scheme is very close to that of the centralized estimator.
The IMS MISSION architecture for distributed manufacturing simulation (2002)

This paper presents an overview of a neutral reference architecture for integrating distributed manufacturing simulation systems with each other, with other manufacturing software applications, and with manufacturing data repositories. Other manufacturing software applications include, but are not limited to systems used to: design products; specify processes; engineer manufacturing systems; and manage production. The architecture identifies the software building blocks and interfaces that will facilitate the integration of distributed simulation systems and enable the integration of those systems with other manufacturing software applications. The architecture is being developed as part of the international Intelligent Manufacturing Systems (IMS) MISSION project.
Electrical properties of bone. A review. (1984)

A review of the available data on the electrical properties (resistance, capacitance, dielectric constant, dielectric loss factor, and dissipation factor, etc.) of whole as well as standardized bone specimens suggest that impedance was lowest in the longitudinal direction and highest in the radial direction. This is further evidence of the anisotropic nature of bone. The electrical properties of fully hydrated bone were significantly different from those of dry and partially wet bone and these properties were highly frequency-dependent. Other variables that influence the electrical properties, such as moisture content, principles and methods of measurement, temperature, and pH and conductivity of the immersing fluid, etc., have also been reviewed. Delineation of these variables is important in reporting test results on the electrical properties of bone; only then can the data on different electrical properties of bone reported by various authors be compared. Future research is needed to characterize the effect of age, microstructure, mineral content, and various disease processes on the electrical properties of bone. Such information may lead to new insight on the role of electrical properties on bone remodeling. An understanding of the electrical behavior of bone is also important for the design of electrical stimulation devices and their proper use for maximum osteogenic effect.
SNPbox: web-based high-throughput primer design from gene to genome (2004)

SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.
"Skil Mate" wearable exoskeleton robot (2003)

A wearable exoskeleton robot, Skil Mate, is a new concept that can assist skilled workers in power as well as in skill. Skil Mate is composed of servo mechanisms and haptic devices (tactile or slipping-off sensors/displays), so that it can follow the workers' movement. Skil Mate is suitable for work in hazardous environments such as in space, under sea, etc. The goal of the project is to develop a prototype model of the wearable robot for a spacesuit, which consists of an upper torso assembly and a pair of arms with gloves.
Photophysical Properties of Porphyrin Tapes (2002)

The novel fused Zn(II)porphyrin arrays (Tn, porphyrin tapes) in which the porphyrin macrocycles are triply linked at meso-meso, beta-beta, beta-beta positions have been investigated by steady-state and time-resolved spectroscopic measurements along with theoretical MO calculations. The absorption spectra of the porphyrin tapes show a systematic downshift to the IR region as the number of porphyrin pigments increases in the arrays. The fused porphyrin arrays exhibit a rapid formation of the lowest excited states (for T2, approximately 500 fs) via fast internal conversion processes upon photoexcitation at 400 nm (Soret bands), which is much faster than the internal conversion process of approximately 1.2 ps observed for a monomeric Zn(II)porphyrin. The relaxation dynamics of the lowest excited states of the porphyrin tapes were accelerated from approximately 4.5 ps for the T2 dimer to approximately 0.3 ps for the T6 hexamer as the number of porphyrin units increases, being explained well by the energy gap law. The overall photophysical properties of the porphyrin tapes were observed to be in a sharp contrast to those of the orthogonal porphyrin arrays. The PPP-SCI calculated charge-transfer probability indicates that the lowest excited state of the porphyrin tapes (Tn) resembles a Wannier-type exciton closely, whereas the lowest excited state of the directly linked porphyrin arrays can be considered as a Frenkel-type exciton. Conclusively, these unique photophysical properties of the porphyrin tapes have aroused much interest in the fundamental photophysics of large flat organic molecules as well as in the possible applications as electric wires, IR sensors, and nonlinear optical materials.
Purification and Characterization of Crotonase from Clostridium acetobutylicum (1972)

An enzyme catalyzingthe reversible hydration of crotonyl-CoA has been obtained in homogeneous form from Clostridium acetobutylicum.
Submilliamp vertical-cavity surface-emitting lasers with buried lateral-current confinement (1995)

Lateral current confinement of intracavity contacted 0.98 μm vertical cavity surface emitting lasers has been achieved by inserting a 50 nm thick n-type GaAs current blocking layer into the p-doped cladding. Epitaxial regrowth by solid source MBE is used to bury the current confining layer in the cavities. Threshold currents of 0.78 mA, optical output powers of 1 mW and external quantum efficiencies of up to 15% are measured. The lateral current injection via the top p-type cladding leads to low voltages at threshold of 1.78 V.
A 22-nucleotide spliced leader sequence in the human parasitic nematode Brugia malayi is identical to the trans-spliced leader exon in Caenorhabditis elegans. (1988)

The mRNAs encoding a 63-kDa antigen in the human parasitic nematode Brugia Malayi contain a spliced leader sequence of 22 nucleotides (nt) that is identical to the trans-spliced leader found on certain actin mRNAs in the distantly related nematode Caenorhabditis elegans. The 22-nt sequence does not appear to be encoded near the 63-kDa genes but is present in multiple copies in several locations within the parasite genome, including the 5S rRNA gene repeat. The 5S-linked copies of the 22-nt sequence are transcribed to yield a 109-nt nonpolyadenylated RNA with the 22-nt leader sequence at its 5' end. We suggest that the 22-nt leader is acquired by 63-kDa antigen mRNAs through trans-splicing. These results indicate that trans-splicing is widespread in nematodes and argue for the functional significance of the 22-nt spliced leader exon in nematode mRNA metabolism.
Scar Dechanneling (2015)

Scar dechanneling alone results in low recurrence and mortality rates in more than half of patients despite the limited ablation extent required. Residual inducible VT ablation improves acute results, but patients who require it have worse outcomes. Recurrences are mainly related to incomplete CC-electrogram elimination.
Timetraveler (2010)

As chip multiprocessors emerge as the prevalent microprocessor architecture, support for debugging shared-memory parallel programs becomes important. A key difficulty is the programs' nondeterministic semantics due to which replay runs of a buggy program may not reproduce the bug. The non-determinism stems from memory races where accesses from two threads, at least one of which is a write, go to the same memory location. Previous hardware schemes for memory race recording log the predecessor-successor thread ordering at memory races and enforce the same orderings in the replay run to achieve deterministic replay. To reduce the log size, the schemes exploit transitivity in the orderings to avoid recording redundant orderings. To reduce the log size further while requiring minimal hardware, we propose Timetraveler which for the first time exploits acyclicity of races based on the key observation that an acyclic race need not be recorded even if the race is not covered already by transitivity. Timetraveler employs a novel and elegant mechanism called post-dating which both ensures that acyclic races, including those through the L2, are eventually ordered correctly, and identifies cyclic races. To address false cycles through the L2, Timetraveler employs another novel mechanism called time-delay buffer which delays the advancement of the L2 banks' timestamps and thereby reduces the false cycles. Using simulations, we show that Timetraveler reduces the log size for commercial workloads by 88% over the best previous approach while using only a 696-byte time-delay buffer.
Introgression of Pythium root rot resistance gene into Rwandan susceptible common bean cultivars (2011)

A breeding scheme was carried out to introgress resistance genes to bean Pythiumroot rot in various commercial varieties grown in Rwanda. The achieved crosses were performed between three selected susceptible varieties (R617-97A, RWR 1668 and Urugezi) which are adapted to the various ecological production zones of Rwanda and two known sources of resistance to Pythium root rot(RWR719 and AND 1062). Following each inter varietal hybridization generation, a series of 4 successive back-crosses was achieved by using the susceptible parents as the initial parent lines to be improved for their respective behavior to Pythium root rot disease. At each back-cross generation, the PYAA 19800 SCAR marker linked toPythium root rot resistance in the two sources of resistance (varieties RWR 719 and AND 1062) was used to identify and to proceed to early selection of progenies possessing the gene of interest. The target materials serving for the molecular analyses were prepared from young trifoliate leaves of 2-weeks bean plantlets. It was observed that at each back-cross generation, there were variable proportions of plants exhibiting presence of the resistance gene according to the SCAR marker profiles. In addition to that observation mainly based to molecular profile, it was also revealed that the proportion of bean seeds having the same color as the susceptible parent line was increasing progressively. Finally, to assess if the individual plants exhibiting the SCAR marker are effectively resistant to the Pythium root rot disease, inoculation tests were carried out with a Pythium ultimum strain on each of them. This ultimate biological evaluation revealed that all the plants showing the SCAR marker were resistant to development of Pythium root rot symptoms after inoculation, confirming thus real introgression of the resistance characteristics through the breeding scheme adopted in our work.

	 

	Key words: Phaseolus, vulgaris, Pythium, molecular, root rot, bean, genotype, resistance.
Fault tolerant digital systems (1989)

Two common dependability measures of fault-tolerant systems, availability and reliability, are defined, and the design of a reliable system is described. The first step is the incoming inspection of components to remove weak or defective components prior to assembly into subsystems. The next step is to use fault-avoidance techniques. Examples are careful signal routing, shielding, cabinet grounding, and inline static filters to increase the signal-to-noise ratio, limiting the fanout of gates to a small number to reduce the dissipation of power, and minimization of human errors through such measures as labeling and documentation. Another technique for increasing reliability is the use of a fault-tolerant design. Three approaches are examined: (1) fault-detection techniques (duplication, coding techniques, the check sum method, watchdog timers and timeouts, and consistency and capability checking); (2) fault-masking techniques (triple modular redundancy and error-correcting codes); (3) dynamic redundancy techniques. System diagnosis and the application of fault-tolerant systems are also discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Packet-Switched On-Chip FPGA Overlay Networks (2006)

As we scale to larger chip capacities, it becomes possible to map large, concurrent applications to programmable fabrics. These applications often have irregular and dynamic communication requirements. Packet-switched networks provide efficient implementations for such applications on these fabrics. In this research, we show how to engineer high-performance packet-switched on-chip networks and provide quantitative comparisons between different kinds of these networks. We analyse different network topologies and justify selection of topologies based on experimental results. We investigate packet-switched and time-multiplexed styles of routing and provide guidance on which style is appropriate for which application.
Low-Frequency Noise in Submicrometer MOSFETs With HfO&lt;tex&gt;$_2$&lt;/tex&gt;, HfO&lt;tex&gt;$_2/hbox Al_2hbox O_3$&lt;/tex&gt;and HfAlO&lt;tex&gt;$_x$&lt;/tex&gt;Gate Stacks (2004)

Low-frequency noise measurements were performed on p- and n-channel MOSFETs with HfO/sub 2/, HfAlO/sub x/ and HfO/sub 2//Al/sub 2/O/sub 3/ as the gate dielectric materials. The gate length varied from 0.135 to 0.36 /spl mu/m with 10.02 /spl mu/m gate width. The equivalent oxide thicknesses were: HfO/sub 2/ 23 /spl Aring/, HfAlO/sub x/ 28.5 /spl Aring/ and HfO/sub 2//Al/sub 2/O/sub 3/ 33 /spl Aring/. In addition to the core structures with only about 10 /spl Aring/ of oxide between the high-K dielectric and silicon substrate, there were "double-gate oxide" structures where an interfacial oxide layer of 40 /spl Aring/ was grown between the high-K dielectric and Si. DC analysis showed low gate leakage currents in the order of 10/sup -12/ A(2-5 /spl times/ 10/sup -5/ A/cm/sup 2/) for the devices and, in general, yielded higher threshold voltages and lower mobility values when compared to the corresponding SiO/sub 2/ devices. The unified number-mobility fluctuation model was used to account for the observed 1/f noise and to extract the oxide trap density, which ranged from 1.8 /spl times/ 10/sup 17/ cm/sup -3/ eV/sup -1/ to 1, 3 /spl times/ 10/sup 19/ cm/sup -3/ eV/sup -1/ somewhat higher compared to conventional SiO/sub 2/ MOSFETs with the similar device dimensions. There was no evidence of single electron switching events or random telegraph signals. The aim of this paper is to present a general discussion on low-frequency noise characteristics of the three different high-K/gate stacks, relative comparison among them and to the Si-SiO/sub 2/ system.
Prototype Real-Time Monitor: Design. (1987)

This report describes the software design used to implement the prototype real-time monitor (RTM) requirements [D'Ippolito 87].The design is presented at three levels: system level, object level, and package architecture level.The report concludes with a discussion of the key implementation obstacles that had to be overcome to develop a working prototype: determining system addresses, communicating with an executing application, accessing application memory, converting data into human readable form, and distributed CPU architectures. Structure of the DocumentChapter 1 of this document gives an overview of the monitoring problem and compares it to other areas where "monitor-like" approaches are used.Chapter 2 provides the high-level architecture for the RTM application system.Chapter 3 examines the objects used to implement the functional needs of the user by proceeding from the lowest to the highest level of abstraction in the system, constantly keeping in mind the needs of the user and building on top of each layer of abstraction.Chapter 4 takes a more "formal" view of the system.Here, object dependency diagrams describe the software architecture (i.e., packaging structure) used to implement the objects described in Chapter 3. Finally, Chapter 5 discusses the key technical issues involved with implementing the RTM, including issues related to performance and use of the RTM in a multiple CPU configuration.
New approach to high-precision Fourier transform spectrometer design (1996)

Laser fringes have long been used to establish the x axis in interferometric spectrometry, but solutions for the intensity axis have been less satisfactory. Now we are seeing the rapid commercial development of low-cost, medium-speed, sigma-delta analog-to-digital converters developed for stereo audio applications. A single chip provides two channels of 20-bit precision at 50 kHz, a significant improvement over many current systems of much greater cost and complexity. But while the laser works in the spatial domain, this converter operates strictly in the time domain; it cannot be triggered. I have developed a bridge between these two domains, the adaptive digital filter, which not only permits us to use this converter to obtain measurements at arbitrary times but as a bonus shows us how to move much of the complexity of an interferometric-control and data-acquisition system from hardware to software. For example, flexible fringe subdivision (to increase the free spectral range) is easily obtained with a simple and efficient algorithm, completely free of laser ghosts. Compensation for drive velocity variation is also possible, requiring only a modest increase in computer memory.
Production and Purification of the Extracellular Ribonuclease of Bacillus Amyloliquefaciens (Barnase) and its Intracellular Inhibitor (Barstar) II. Barstar (1972)

Abstract Procedures are reported for the extraction and purification of barstar, the intracellular inhibitor of barnase, the extracellular ribonuclease of Bacillus amyloliquefaciens, (a strain formerly erroneously classified as B. subtilis, strain H), from frozen acidified cells obtained during the isolation of barnase1. Methods include affinity chromatography on agarose to which barnase is covalently bound. Experiments relating to the purity, molecular weight, and amino acid composition of the product are reported.
Cytotoxic and radiosensitizing effects of hypoxic cell sensitizers on EMT6 mouse mammary tumour cells in vivo and in vitro. (1978)

The radiosensitizing activities and toxicities of misonidazole, metronidazole, 2-Methythio-3-nitropyrrole (3NPR), 2-amino-5-nitrothiazole (ANT) and 2,5-dinitroimidazole (KA121) were studied using EMT6 tumour cells and BALB/c KaRw mice. For 10(-4)M concentrations of the drugs, the DMF's for hypoxic cells were: Misonidazole, 1.6; 3NPR, 1.5, ANT, 1.5; KA121, 1.8. The OER was 3.2. In general, the drugs were toxic to hypoxic cells and were not toxic to aerobic cells at 10(-3)M for 24 h. The LD50's in BALB/c KaRw mice were: misonidazole, 1500 mg/kg; 3NPR, 350 mg/kg ANT, 420 mg/kg. In vivo, 1 mg/g misonidazole or metronidazole reduced the terminal slope of the tumour cell survival curve by a factor of 2.2 or 1.6 respectively.