gebhart/patrepeval huggingface dataset:
Managing the Coexistence of Hierarchical and Collegial Governance Structures (1985)

Dual structures of governance, that is, the collegial and the hierarchical, are in place in many university libraries today. The problems from a managerial perspective of managing the coexistence of these governance structures may include accountability, lack of organizational flexibility, time requirements, communication between the structures, some lessening of librarians' interest in the function of line management, the potential for goal conflict, and a lack of appreciation for librarians' position in their bargaining group. While there is no one solution to these problems, it is imperative that university libraries operate in such a manner as to be judged effective or the mandate of the library stands in some danger of being at least partially distributed.
Isolation and Structure of Hemibastadinols 1−3 from the Papua New Guinea Marine Sponge <i>Ianthella </i><i>basta</i> (1996)

Further investigation of the Bismarck Archipelago (Papua New Guinea) marine sponge Ianthella basta for biologically active constituents has led to the isolation of hemibastadins 1 (2), 2 (3), and 3 (4) and the new brominated tyrosine derivatives hemibastadinols 1-3 (9, 13, and 14). Isolation and structure elucidation of the monomethyl ether derivatives (7 and 8) of hemibastadins 1 and 2 and the 3-bromotyramine amide of oxalic acid amide (1a) concluded our chemical investigation of I. basta. The hemibastadins and hemibastadinols represent important biosynthetic links to a series of bromotyrosine tetramers collectively known as the bastadins. The antimicrobial activity of the bastadins, hemibastadins, and hemibastadinols is summarized.
Yttrium Alkyl and Benzyl Complexes with Amino−Amidinate Monoanionic Ancillary Ligands (2000)

The sequential reaction of YCl3(THF)3.5 with [t-BuC(Ni-Pr)2]Li (Li[A]) and 2 equiv of LiCH(SiMe3)2 gave the mono(amidinate) dialkyl yttrium complex [t-BuC(Ni-Pr)2]Y[CH(SiMe3)2]2(μ-Cl)Li(THF)3 (1), which was structurally characterized. Use of the potentially tridentate amidinate−amine ligands [PhC(NSiMe3)N(CH2)nNMe2]- (n = 2, B; 3, C) yielded the salt-free dialkyl complexes [PhC(NSiMe3)N(CH2)nNMe2]Y[CH(SiMe3)2]2 (n = 2, 2a; 3, 2b). A structure determination of 2a showed the tridentate ligand to adopt an approximately meridional geometry. Attempts to prepare analogous benzyl or trimethysilylmethyl complexes with ligand B yielded the ate-complexes Li[PhC(NSiMe3)N(CH2)2NMe2]2Y(R)2 (R = CH2Ph, 3a; CH2SiMe3, 3b). A crystal structure of 3a showed that the Li ion is encapsulated by two amidinate and two amine nitrogens. The increased spacer length between the amidinate and amine functionalities in ligand C prevented encapsulation of the Li ion, but still produced a bis(amidinate) yttrium benzyl complex, [PhC(NSiMe3)N(CH2)3NMe2]2YCH2Ph (4). In this compound only one of the two dimethylamino functionalities is coordinated to the metal center.
Evolutionary history and metabolic insights of ancient mammalian uricases (2014)

Uricase is an enzyme involved in purine catabolism and is found in all three domains of life. Curiously, uricase is not functional in some organisms despite its role in converting highly insoluble uric acid into 5-hydroxyisourate. Of particular interest is the observation that apes, including humans, cannot oxidize uric acid, and it appears that multiple, independent evolutionary events led to the silencing or pseudogenization of the uricase gene in ancestral apes. Various arguments have been made to suggest why natural selection would allow the accumulation of uric acid despite the physiological consequences of crystallized monosodium urate acutely causing liver/kidney damage or chronically causing gout. We have applied evolutionary models to understand the history of primate uricases by resurrecting ancestral mammalian intermediates before the pseudogenization events of this gene family. Resurrected proteins reveal that ancestral uricases have steadily decreased in activity since the last common ancestor of mammals gave rise to descendent primate lineages. We were also able to determine the 3D distribution of amino acid replacements as they accumulated during evolutionary history by crystallizing a mammalian uricase protein. Further, ancient and modern uricases were stably transfected into HepG2 liver cells to test one hypothesis that uricase pseudogenization allowed ancient frugivorous apes to rapidly convert fructose into fat. Finally, pharmacokinetics of an ancient uricase injected in rodents suggest that our integrated approach provides the foundation for an evolutionarily-engineered enzyme capable of treating gout and preventing tumor lysis syndrome in human patients.
Glypican-3 immunocytochemistry in liver fine-needle aspirates (2007)

Results indicated positive staining for GPC3 as defined in 90% of liver FNAs from HCC patients. All metastatic tumors and benign aspirates studied were negative for GPC3. ICC was superior to IHC in 25% of cases. This pilot study supports the diagnostic utility of GPC3 in hepatic FNAs to aid in distinction of HCC from metastatic tumors and benign liver lesions.
Challenges of antibacterial discovery revisited (2010)

The discovery of novel antibiotic classes has not kept pace with the growing threat of bacterial resistance. Antibiotic candidates that act at new targets or via distinct mechanisms have the greatest potential to overcome resistance; however, novel approaches are also associated with higher attrition and longer timelines. This uncertainty has contributed to the withdrawal from antibiotic programs by many pharmaceutical companies. Genomic approaches have not yielded satisfactory results, in part due to nascent knowledge about unprecedented molecular targets, the challenge of achieving antibacterial activity by lead optimization of enzyme inhibitors, and the limitations of compound screening libraries for antibacterial discovery. Enhanced diversity of compound screening banks, entry into new chemical space, and new screening technologies are currently being exploited to improve hit rates for antibacterial discovery. Antibacterial compound lead optimization faces hurdles associated with the high plasma exposures required for efficacy. Lead optimization would be enhanced by the identification of new antibiotic classes with improved tractability and by expanding the predictability of in vitro safety assays. Implementing multiple screening and target identification strategies is recommended for improving the likelihood of discovering new antibacterial compounds that address unmet needs.
Fast software processing of motion JPEG video (1994)

This paper introduces a set of techniques for processing video data compressed using JPEG compression at near real-time rates on current generation workstations. Performance is improved over traditional methods by processing video data in compressed form, avoiding compression and decompression and reducing the amount of data processed. An approximation technique called condensation is developed that further reduces the complexity of the operation. The class of operations that are computable using the techniques developed in this paper are called linear, global digital specials effects (LGDSEs), and represent those effects where a pixel in the output image is a linear combination of pixels in the input image. Many important video processing problems, including convolution, scaling, rotation, translation, and transcoding can be expressed as LGDSEs.
Interpolation of lowpass signals at half the Nyquist rate (1996)

Describes the interpolation of low-pass signals from a class of stable sampling sets at half the Nyquist rate. Practical reconstruction algorithms are also suggested.
LARGE EDDY SIMULATION OF ORGANIZED STRUCTURE AND MOMENTUM TRANSFER WITHIN AND ABOVE A PLANT CANOPY (1993)

大気―植生境界に生じる大規模乱流の渦構造と運動量交換の関係を解明するためLESモデルを構築し検討した. このモデルでは, 葉の抵抗を考慮した運動方程式と同時に, 葉による wake production と dissipation の効果を含んだ格子スケール以下の乱流エネルギト収支式を連立して解く. 計算結果から, ローラーとリブで特徴づけられる3次元組織構造が大気―植生境界に生成されること, 植生層内で sweep 型乱れが卓越すること, 現地観測で報告されている乱流統計的性質, それらと渦構造との関連性が説明された.
Structural Basis for Differences in Substrate Selectivity in Kex2 and Furin Protein Convertases<sup>,</sup> (2004)

Kex2 is the yeast prototype of a large family of serine proteases that are highly specific for cleavage of their peptide substrates C-terminal to paired basic sites. This paper reports the 2.2 A resolution crystal structure of ssKex2 in complex with an Ac-Arg-Glu-Lys-Arg peptidyl boronic acid inhibitor (R = 19.7, R(free) = 23.4). By comparison of this structure with the structure of the mammalian homologue furin [Henrich, S., et al. (2003) Nat. Struct. Biol. 10, 520-526], we suggest a structural basis for the differences in substrate recognition at the P(2) and P(4) positions between Kex2 and furin and provide a structural rationale for the lack of P(6) recognition in Kex2. In addition, several monovalent cation binding sites are identified, and a mechanism of activation of Kex2 by potassium ion is proposed.
Indicator displacement assays: from concept to recent developments (2021)

Overcoming the synthetic burden related to covalently connected receptors with appropriate indicators for sensing various analytes via an indicator spacer receptor (ISR) approach, the indicator displacement assay (IDA) seems to be a very sophisticated and versatile supramolecular sensing paradigm, and it has taken the phenomenon of molecular recognition to the next level in the realm of host-guest chemistry. Due to the unavailability of a comprehensive report on what has been done in the last decade in relation to IDAs, we decided to set down this account illustrating diverse indicator displacement assays (IDAs) in detail from the concept stage to recent developments relating to the detection of cationic, anionic, and neutral analytes. The authors conclude this account with future perspectives and highlight the limitations and challenges relating to IDAs which need to be overcome in order to realize the full potential of this popular sensing phenomenon. While we were finalizing our account for publication, a tutorial review by the research groups of Anslyn, Sessler, and Sun was published, which focuses mainly on diverse aspects of the chemistry related to IDAs. As can be seen, our review, besides discussing various basic IDA concepts, has a vast collection of information published in the past decade and hence, hopefully, will be very informative for the supramolecular community. We believe that this work will offer new insights for the construction of novel sensors operating through the IDA approach.
Integration of air gaps based on selective ozone/TEOS deposition into a multi layer metallization scheme (2006)

The electrical properties of copper metal lines within an air gap scheme were measured and compared to structures without air gaps. Two metal layers with air gap structures using a close-off processing scheme with selective ozone TEOS deposition were fabricated. With these air gaps, an effective k of as low as 2.3 could be achieved
Structure of the FBJ murine osteosarcoma virus genome: molecular cloning of its associated helper virus and the cellular homolog of the v-fos gene from mouse and human cells. (1983)

The 8.2-kilobase (kb) unintegrated circular DNA form of the FBJ murine leukemia virus (FBJ-MLV) was linearized by cleavage at the single HindIII site, molecularly cloned into bacteriophage Charon 30, and subsequently subcloned into pBR322 (pFBJ-MLV-1). Both FBJ-MLV virion RNA and pFBJ-MLV-1 DNA were used to investigate the arrangement of helper virus sequences in the FBJ murine osteosarcoma virus genome (FBJ-MSV) by heteroduplex formation with cloned FBJ-MSV proviral DNA. The results showed that the FBJ-MSV genome contained 0.8 kb of helper virus sequence at its 5' terminus and 0.98 kb at its 3' terminus. Approximately 6.8 kb of helper virus sequence had been deleted, and 1.7 kb of unrelated sequence was inserted into the FBJ-MSV genome. This substituted region contains v-fos, the transforming gene of FBJ-MSV. Using a probe specific for v-fos, we have cloned homologous sequences (c-fos) from mouse and human chromosomal DNA. Heteroduplex analysis of FBJ-MSV DNA with these recombinant clones showed that both the c-fos(mouse) and the c-fos(human) sequences hybridized to the entire 1.7-kb v-fos region. However, five regions of homology of 0.27, 0.26, 0.14, 0.5, and 0.5 kb were separated by four regions of nonhomology of 0.76, 0.55, 0.1, and 0.1 kb from 5' to 3' with respect to the FBJ-MSV genome. The size of these sequences showed striking similarity in both c-fos(mouse) and c-fos(human).
<i>DICER1</i> syndrome: clarifying the diagnosis, clinical features and management implications of a pleiotropic tumour predisposition syndrome (2011)

Constitutional DICER1 haploinsufficiency predisposes to a broad range of tumours, making a substantial contribution to PPB, cystic nephroma and ovarian Sertoli-Leydig tumours, but a smaller contribution to other tumours. Most mutation carriers are unaffected, indicating that tumour risk is modest. The authors define the clinical contexts in which DICER1 mutation testing should be considered, the associated tumour risks, and the implications for at-risk individuals. They have termed this condition 'DICER1 syndrome'. ACCESSION NUMBERS: The cDNA Genbank accession number for the DICER1 sequence reported in this paper is NM_030621.2.
Characterization and chromosomal localization of the cornea-specific murine keratin gene Krt1.12. (1994)

Keratins are a group of water-insoluble proteins constituting paired acidic and basic keratin molecules that form 10-nm intermediate filaments in epithelial cells. Expression of the K3/K12 keratin pair characterizes the cornea-type differentiation. However, the mechanism that regulates this cornea-specific K12 expression remains unknown. To provide a better understanding of the cornea-specific expression, we have cloned the K12 cDNA (Liu, C.-Y., Zhu, G., Westerhausen-Larson, A., Converse, R., Kao, C. W.-C., Sun, T.-T., and Kao, W. W.-Y. (1993) Curr. Eye Res. 12, 963-974). In present studies, the murine K12 keratin gene (Krt1.12) was isolated and characterized. The murine Krt1.12 gene spans 6,567 base pairs of genomic DNA, and the mRNA encoding K12 keratin is distributed into eight exons. Chromosome mapping reveals that murine Krt1.12 is located within the Krt1 complex of mouse chromosome 11. In addition to the production of authentic K12 mRNA, the Krt1.12 gene gives rise to several alternate poly(A)+ RNAs by the use of alternative splicing in intron 2, an alternative promoter in intron 1, and/or both. Sequence analysis indicates that the transcripts derived from alternative splicing and/or the alternative promoter do not have a long open reading frame for keratin or keratin-like molecules. It is not known whether these alternate K12 poly(A)+ RNAs have any biological functions, e.g. regulation of K12 gene expression.
Standardization of Criteria for Adolescent Idiopathic Scoliosis Brace Studies (2005)

Optimal inclusion criteria for future adolescent idiopathic scoliosis brace studies consist of: age is 10 years or older when brace is prescribed, Risser 0-2, primary curve angles 25 degrees -40 degrees , no prior treatment, and, if female, either premenarchal or less than 1 year postmenarchal. Assessment of brace effectiveness should include: (1) the percentage of patients who have < or =5 degrees curve progression and the percentage of patients who have > or =6 degrees progression at maturity, (2) the percentage of patients with curves exceeding 45 degrees at maturity and the percentage who have had surgery recommended/undertaken, and (3) 2-year follow-up beyond maturity to determine the percentage of patients who subsequently undergo surgery. All patients, regardless of subjective reports on compliance, should be included in the results (intent to treat). Every study should provide results stratified by curve type and size grouping.
Evidence for a novel mechanism for gene amplification in multiple myeloma: 1q12 pericentromeric heterochromatin mediates breakage‐fusion‐bridge cycles of a 1q12∼23 amplicon (2009)

Gene amplification is defined as a copy number (CN) increase in a restricted region of a chromosome arm, and is a mechanism for acquired drug resistance and oncogene activation. In multiple myeloma (MM), high CNs of genes in a 1q12 approximately 23 amplicon have been associated with disease progression and poor prognosis. To investigate the mechanisms for gene amplification in this region in MM, we performed a comprehensive metaphase analysis combining G-banding, fluorescence in situ hybridization, and spectral karyotyping in 67 patients with gain of 1q. In six patients (9%), evidence for at least one breakage-fusion-bridge (BFB) cycle was found. In three patients (4%), extended ladders of 1q12 approximately 23 amplicons were identified. Several key structures that are predicted intermediates in BFB cycles were observed, including: equal-spaced organization of amplicons, inverted repeat organization of amplicons along the same chromosome arm, and deletion of sequences distal to the amplified region. The 1q12 pericentromeric heterochromatin region served as both a recurrent breakpoint as well as a fusion point for sister chromatids, and ultimately bracketed both the proximal and distal boundaries of the amplicon. Our findings provide evidence for a novel BFB mechanism involving 1q12 pericentromeric breakage in the amplification of a large number of genes within a 1q12 approximately 23 amplicon.
Automatic generation of quadrilateral elements by a conformal mapping (1985)

A finite element mesh generator is described based on a conformal mapping which requires little more than the definition of boundary geometry to generate a mesh. The method is restricted to plane regions which are simply connected. The interior of a region containing a uniform mesh of regularly shaped 8‐node quadrilateral elements is mapped conformally to the physical domain with the result that bandwidth is automatically minimized and that smooth transitions are made between large and small elements. Although the procedure is not satisfactory for general applications, most common geometrical shapes can be modelled with meshes of good quality. The method is based primarily on boundary data but the user can specify a region of high mesh density. Examples are given to illustrate typical results.
Esters of N-tert-butylarterenol. Long-acting new bronchodilators with reduced cardiac effects (1976)

The preparation of various esters of N-tert-butylarterenol is described. Esterification of the phenolic OH groups has increased bioavailability, prolonged bronchodilation, and reduced tachycardia. The substitution of aromatic esters compared with simple aliphatic esters improved markedly these pharmacological properties. Of a number of esters tested, compound 45 (bitolterol) demonstrated the most favorable pharmacological properties as a bronchodilator. Its long duration of action and significant bronchodilator-cardiovascular separation are briefly described.
Generation of ultrashort hard-x-ray pulses with tabletop laser systems at a 2-kHz repetition rate (2003)

We describe the generation of ultrafast x-ray pulses at a 2-kHz repetition rate with a new tabletop laser and x-ray generation system. The range of the emitted x-radiation wavelength is approximately a single ångstrom, from 0.3 to 0.1 nm. Specific emphasis is placed on the generation of broad continuum radiation for x-ray absorption spectroscopy. High-contrast laser pulses are desirable for generation of ultrashort pulses, which we achieve by dividing the laser system into a section for generation of high-contrast microjoule pulses followed by pulse cleaning and a section for chirped pulse amplification and pulse compression. Using this system, we generated x radiation from solid-copper and liquid-mercury targets in a helium atmosphere. From copper targets an average x-ray flux of 1013 photons/(s 4π sr keV) at 3 keV and 109 photons/(s 4π sr) above 5 keV photon energy was produced. X radiation from the mercury target did not exhibit emission lines within the spectral range 3–13 keV.
Microfluidic Strategies for Design and Assembly of Microfibers and Nanofibers with Tissue Engineering and Regenerative Medicine Applications (2014)

Fiber-based materials provide critical capabilities for biomedical applications. Microfluidic fiber fabrication has recently emerged as a very promising route to the synthesis of polymeric fibers at the micro and nanoscale, providing fine control over fiber shape, size, chemical anisotropy, and biological activity. This Progress Report summarizes advanced microfluidic methods for the fabrication of both microscale and nanoscale fibers and illustrates how different methods are enabling new biomedical applications. Microfluidic fabrication methods and resultant materials are explained from the perspective of their microfluidic device principles, including co-flow, cross-flow, and flow-shaping designs. It is then detailed how the microchannel design and flow parameters influence the variety of synthesis chemistries that can be utilized. Finally, the integration of biomaterials and microfluidic strategies is discussed to manufacture unique fiber-based systems, including cell scaffolds, cell encapsulation, and woven tissue matrices.
Prefrontal cortical network connections: key site of vulnerability in stress and schizophrenia (2011)

The symptoms of schizophrenia involve profound dysfunction of the prefrontal cortex (PFC). PFC networks create our "mental sketch pad", and PFC dysfunction contributes to symptoms such as cognitive deficits, thought disorder, delusions and hallucinations. Neuropathological studies of schizophrenia have shown marked loss of dendritic spines in deep layer III, the sublayer where PFC microcircuits reside. The microcircuits consist of recurrent excitatory pyramidal cell networks that interconnect on spines, and excite each other via NMDA receptor signaling. The pyramidal cell persistent firing is sculpted by lateral inhibition from GABAergic basket and chandelier cells, thus creating tuned, persistent firing needed for accurate representational knowledge (i.e., working memory). The strength of pyramidal cell network connections is markedly and flexibly altered by intracellular signaling pathways in dendritic spines, a process called dynamic network connectivity (DNC). DNC proteins such as HCN channels are concentrated on dendritic spines in deep layer III. Under optimal conditions, network inputs to pyramidal cells are strengthened by noradrenergic alpha-2A inhibition of cAMP-HCN channel signaling, and sculpted by dopamine D1-cAMP-HCN channel weakening of inappropriate inputs. However, with stress exposure, high levels of cAMP-HCN channel signaling produces a collapse in network firing. With chronic stress exposure, spines reduce in size and are lost, and this process involves increased PKC signaling. Importantly, molecules that normally strengthen PFC networks connections and/or reverse the stress response, are often genetically altered in schizophrenia. As exposure to stress is a key factor in the precipitation of schizophrenic symptoms, these dysregulated signaling pathways in deep layer III may interact with already vulnerable circuitry to cause spine loss and the descent into illness.