gebhart/patrepeval huggingface dataset:
Isolation of mannan-protein complexes from viable cells of Saccharomyces cerevisiae X2180-1A wild type and Saccharomyces cerevisiae X2180-1 A-5 mutant strains by the action of Zymolyase-60,000 (1983)

The viable whole cells of Saccharomyces cerevisiae X2180-1A wild type and its mannan mutant strain S. cerevisiae X2180-1A-5, were treated with an Arthrobacter sp. beta-1,3-glucanase in the presence of a serine protease inhibitor, phenyl-methylsulfonyl fluoride. Fractionation of the solubilized materials of each strain with Cetavlon (cetyltrimethylammonium bromide) yielded one mannan-protein complex. Molecular weights of these complexes were almost the same as that of the mannoprotein of the mutant strain prepared by Nakajima and Ballou, which had a molecular weight of 133,000 and were approximately three times larger than those of the mannans isolated from the same cells by hot-water extraction. Each mannan-protein complex contained up to 2% glucose residue, which was not removed by specific precipitation with anti-mannan sera or by affinity chromatography on a column of concanavalin A-Sepharose. Treatment of these complexes with alkaline NaBH4 produced peptide-free mannan containing small amounts of glucose nearly identical to those of the parent complexes. The above findings provide evidence that the glucose residues exist in a covalently linked form to the mannan moiety. Fractionation of the mannan-protein complex of the S. cerevisiae wild-type strain by DEAE-Sephadex chromatography yielded five subfractions of different phosphate content, indicating that these highly intact mannan-protein complexes were of heterogeneous material consisting of many molecular species of different phosphate content.
BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor (2001)

Mutational inactivation of BRCA1 confers a cumulative lifetime risk of breast and ovarian cancers. However, the underlying basis for the tissue-restricted tumor-suppressive properties of BRCA1 remains poorly defined. Here we show that BRCA1 mediates ligand-independent transcriptional repression of the estrogen receptor alpha (ERalpha), a principal determinant of the growth, differentiation, and normal functional status of breasts and ovaries. In Brca1-null mouse embryo fibroblasts and BRCA1-deficient human ovarian cancer cells, ERalpha exhibited ligand-independent transcriptional activity that was not observed in Brca1-proficient cells. Ectopic expression in Brca1-deficient cells of wild-type BRCA1, but not clinically validated BRCA1 missense mutants, restored ligand-independent repression of ERalpha in a manner dependent upon apparent histone deacetylase activity. In estrogen-dependent human breast cancer cells, chromatin immunoprecipitation analysis revealed the association of BRCA1 with ERalpha at endogenous estrogen-response elements before, but not after estrogen stimulation. Collectively, these results reveal BRCA1 to be a ligand-reversible barrier to transcriptional activation by unliganded promoter-bound ERalpha and suggest a possible mechanism by which functional inactivation of BRCA1 could promote tumorigenesis through inappropriate hormonal regulation of mammary and ovarian epithelial cell proliferation.
<title>Progress in noninvasive optical diagnostics</title> (2000)

It may look odd that a session on noninvasive diagnostks is scheduled as a part of an in— vitro diagnostic instrumentation symposium. Hopefully, NI diagnostics will grow to the extent that we will have several sessions on different aspects of it in future meetings. In this talk I will discuss the general field of non-invasive diagnostics from industry perspective with examples on what are the advances in hemoglobin and glucose measurements. The other two talks in this session will deal with NI optical devices for bilirubin (SpectRx) and Hb/Hct (In-Line Diagnostics) that just achieved regulatory clearance and are ready for the market. A tissue oxygenation photon density waves photometer for tissue oxygenation measurements (ISS Instruments) and a brain oxygen saturation instrument (Hamamatsu) are available commercially for research purposes. It was quite encouraging to see how a novel technology as optical coherence tomography made its way from the research lab to a clinical product for ophthalmic applications, the workshop arranged on this topic was quiet interesting. SPIE had several sessions on the past few years on optical biopsy, laser interaction with tissue, optical coherence tomography, photoacoustics interactions in tissues and photon migrations in tissues. Some of these techniques will eventually find their way to the hospital floor, the ICU and the doctors office. Some of the constant participants in this annual BIOS/SPIE meeting as Chance, Jacques, Gratton, Alfano, Tromberg, Delpy, Fujimuto, Ezzat, and others have laid the scientific foundation of optical diagnostics, practical applications may be soon on the horizon.
Apparent Diffusion Coefficient Scalars Correlate with Near-Infrared Spectroscopy Markers of Cerebrovascular Autoregulation in Neonates Cooled for Perinatal Hypoxic-Ischemic Injury (2014)

Blood pressure deviation from the optimal autoregulatory range may be an early biomarker of injury in the posterior centrum semiovale, posterior limb of the internal capsule, and basal ganglia. Optimizing blood pressure to support autoregulation may decrease the risk of brain injury in cooled neonates with hypoxic-ischemic injury.
Directional electromigration-enhanced interdiffususion in the Cu–Ni system (2007)

The effect of a dc on the interdiffusivity D̃ in the Cu–Ni system was investigated over the temperature range of 650–850°C and at current densities in the range of 0–1000Acm−2. Interdiffusivities were calculated using the Sauer–Freise–den Broeder method and the values calculated in the absence of a current were in agreement with previously published results. The influence of the current on D̃ depended on its direction relative to the two interfaces in the trilayered Cu–Ni–Cu samples. When the electronic flow was from Ni to Cu (cocurrent interface), the interdiffusivity showed a marked increase relative to copper content but was unchanged when the electronic flow was from Cu to Ni (countercurrent interface). The increase of D̃ in the cocurrent interface depended on concentration and temperature. At lower temperatures, the increase becomes significant at higher copper concentrations but for the same value of current density, the increase is apparent at lower concentrations. The effective activation energy of interdiffusivity depended on concentration and decreased with the application of a current. The decrease was largest for higher copper concentrations. The results are interpreted in terms of a proposed vacancy-atom interaction for copper with the implication that the electron wind effect on Cu is counteracted by the effect of vacancies.
Portal vein embolization and its effect on tumour progression for colorectal cancer liver metastases (2015)

Abstract Background The aim of this study was to evaluate the long-term outcomes of patients with colorectal cancer liver metastasis (CRCLM) exhibiting disease progression after portal vein embolization (PVE). Methods Patients with CRCLM requiring PVE before hepatectomy between 2003 and 2014 were included. Clinical variables, and liver and tumour volumes determined by three-dimensional CT volumetry were assessed before and after PVE. Overall and disease-free survival data were obtained. Univariable and multivariable logistic regression analyses were performed to identify predictors of tumour progression after PVE. Results Of 141 patients who underwent PVE, 93 (66·0 per cent) had tumour progression and 17 (12·1 per cent) developed new contralateral lesions. Significantly fewer patients had resectable disease in the group with disease progression than among those with stable disease: 43 (46 per cent) of 93 versus 36 (75 per cent) of 48 respectively (P = 0·001). Median survival was similar in patients with and without tumour growth after PVE: 22·5 versus 26·0 months for patients with unresectable tumours (P = 0·706) and 46·2 versus 52·2 months for those with resectable disease (P = 0·953). However, disease-free survival for patients with tumour progression after PVE was shorter than that for patients with stable disease (6·0 versus 20·2 months; P = 0·045). Response to neoadjuvant chemotherapy was the only significant factor associated with tumour progression in multivariable analysis. Conclusion Tumour progression after PVE did not affect overall survival, but patients with resected tumours who had tumour growth after embolization experienced earlier recurrence. A borderline response to neoadjuvant chemotherapy seemed to be associated with tumour progression after PVE.
An Outer-Sphere Two-Electron Platinum Reagent (2003)

A strategy for designing cooperative outer-sphere two-electron platinum reagents is demonstrated. The novel platinum(II) complex, [Pt(tpy)(pip2NCN)][BF4] (1(BF4-)) (tpy = 2,2':6',2' '-terpyridine, pip2NCN- = 2,6-(CH2N(CH2)5)2-C6H3-), in which the metal is bonded to two pincer type ligands, has been prepared. Treatment of 1 with protic acid results in protonation of the pendant piperdyl groups, allowing for the isolation of [Pt(tpy)(pip2NCNH2)][PF6]3 (2(PF6-)3). 1H NMR spectra of 1 and 2 establish that in each complex the terpyridyl ligand is tridentate, whereas the piperdyl ligand is monodentate, bonded to platinum through the phenyl ring. The structure of the protonated complex was confirmed by an X-ray crystallographic study of crystals of 2(Cl-)3.4H2O. The cyclic voltammagram of 1 exhibits two reversible one-electron reduction waves at E degrees ' = -0.98 V and E degrees ' = -1.50 V (E degrees ' = (Epc + Epa)/2), with a DeltaEp of 65 and 61 mV, respectively. In contrast to other Pt(II) complexes, including 2, this complex also undergoes a nearly reversible two-electron oxidation process at E degrees ' = 0.40 V (DeltaEp = 43 mV, 0.01 V/s). The accumulated data are consistent with the unusual ligand architecture of 1 being capable of stabilizing and allowing for facile interconversion between the Pt(II) and Pt(IV) oxidation states.
THE QUANTITATIVE HISTOCHEMISTRY OF THE NORMAL HUMAN LIVER LOBULE. (1965)

Abstract With the use of microdissection and microchemical techniques, measurements were made of protein, desoxyribosenucleic acid, and seven enzymes in periportal and centrolobular areas of the normal human liver lobule. The whole portal tracts and their components were also studied. The enzymes measured participate in one or more systems in the metabolism of carbohydrates: glycolysis, glucogenesis, the pentose pathway, the tricarboxylic acid cycle, and gluconeogenesis. Alkaline phosphatase was also measured. Activities being expressed on either a protein or a desoxyribosenucleic acid basis, phosphoglucoisomerase and lactic dehydrogenase were demonstrated to be more active in periportal areas of the normal, human liver lobule, but isocitric dehydrogenase, ,glucose-6-phosphate dehydrogenase, and alkaline phosphatase were more active in centrolobular areas. Malic dehydrogenase and glutamic-pyruvic transaminase had comparable periportal and centrolobular activities. On both a protein and a desoxyribosenucleic acid basis, the whole portal tract revealed a greater activity for glucose-6-phosphate dehydrogenase than did parenchymal cells, but relatively lower activities for the other six enzymes. A study of the components of the portal tract on a desoxyribosenucleic acid basis revealed that arterioles and venules contribute importantly to the activity of each enzyme in the portal tract as a whole. Halt, ever, in the bile ducts, only alkaline phosphatase, malic dehydrogenase, glucose-6-phosphate dehydrogenase, and phosphoglucoisomerase contributed importantly to the activity in complete portal tracts. These -results in the human liner lobule are compared to our previously reported intralobular distributions of enzyme activities in the normal rat liver. In both species, enzymes participating in anaerobic glycolysis have higher periportal activities, while those participating in the aerobic metabolism of carbohydrates have higher centrolobular activities.
The Enterprise for the America's initiative: issues and prospects for a free trade agreement in the Western Hemisphere (1994)

Introduction by Roy E. Green Global Trade and Regional Economic Development The Free Trade Debate by Patrick Low Liberalizing Trade in the Western Hemisphere: Where Do We Want to Go, and How Do We Get There? by Ronald J. Wonnacott The Canada-U.S. FTA and the Laboratory of the Mexican, U.S., and Canadian NAFTA Negotiations The North American Free Trade Agreement: A Canadian Perspective by Murray G. Smith A U.S. Perspective on the Canada-U.S. Free Trade Agreement by Arlene E. Wilson A Mexican Assessment of the North American Free Trade Agreement Negotiations: Issues and Prospects by Carlos Alba Vega Trade Liberalization and the Lessons of the Mexican Maquiladora Program by Randall Crane The Evolving Experience along the Pacific Northwest Corridor Called Cascadia by Michael A. Goldberg and Maurice D. Levi Keys to a Free Trade Zone in the Western Hemisphere: History, Opportunity, Motivation, and Timing The Rocky Road toward Hemispheric Economic Integration: A Regional Background with Attention to the Future by Joseph Grunwald The Enterprise for the Americas Initiative: Empty Gesture, Shrewd Strategic Gambit, or Remarkable Shift in Hemispheric Relations? by Joseph S. Tulchin The North American Free Trade Negotiating Track: Agenda Setting for the Enterprise for the Americas Initiative Argentina, the Southern Common Market, and the Prospects for Success of the EAI by Roberto Bouzas The Chilean Perception of the Americas Initiative by Mladen Yopo H. An Analysis of Colombian Reactions to the EAI: Prospects for Success by Mauricio Reina and Gladys Cristina Barrera Selected Bibliography Index
Inherent Fingerprint Luminescence—Detection by Laser (1977)

Abstract The currently used methods of fingerprint detection may be classified into two categories: those which are dependent on the adherence of inert materials to fingerprint residues (powder methods), and those which rely on chemical interaction of a detection reagent with specific components of the latent print (for example, ninhydrin method) [1]. Both classes, in fact all conventional fingerprint detection methods, require a chemical or physical treatment of the exhibit under examination.
Rich Structure Chemistry in the Aluminophosphate Family (2003)

This Account describes a family of aluminophosphates ranging from neutral open-frameworks to anionic frameworks with fascinating structural architectures. In contrast to aluminosilicate zeolites, these aluminophosphates exhibit wealthy structural features, including extra-large micropores, mixed bonding, different structural dimensionalities, rich compositional diversities, and various stacking sequences of their 2-D sheets. The low-dimensional networks, acting as building units, can be assembled to 3-D open-frameworks via transition metal cations through a building-up process. New stoichiometries and hypothetical structural topologies can be predicted and enumerated. Computational and combinatorial approaches will greatly help to access the range of the diverse structures.
EScribe:a Topic-based Publish-subscribe System with Dynamic Routing (2012)

Scribe,a classic topic-based publish-subscribe system,disseminates events to the subscribers through distributed multicast trees.However,redundant event deliveries and overstaffed multicast trees are introduced.This paper proposed an Enhanced topic-based publish-subscribe system based on Scribe,namely EScribe,to reduce the costs of event dissemination and multicast tree maintenance.EScribe adopts Bloom filters to check the subscriptions of the Pastry leaf nodes,and dynamically routes the next hop by means of the leaf nodes′ subscriptions.The maintenance interval can be tuned according to the level of a node in the multicast tree.A series of experiments is conducted to demonstrate that EScribe greatly reduces redundant event deliveries and the size of multicast trees,and the dissemination cost and maintenance cost decrease significantly.
A reflectance photometer with a square photodiode array detector for use on multilayer dry-film slides. (1988)

This semiautomated prototype reflectance photometer measures reflected light from multilayer dry-film slides. The instrument makes use of a square photodiode array detector, a Hewlett-Packard desktop computer, and a modified mechanical transport mechanism from an Ektachem DT60 analyzer. When 2 microL of serum is placed on a dry-film slide, a colored spot is formed. The slide is automatically transported to an incubation area and then to the photometer area. There the spot is illuminated with dual tungsten lamps, and the reflected light passes through an interference filter, where it is focused on a square photodiode array containing 10,000 individual detectors. The analog signal from each detector is digitized and transmitted to a computer for calculation of the percentage of reflectance. I used a series of algorithms to locate the spot, estimate spot area, correct for minor variations in sample volume, and compute the average reflectance from a central spot area. To evaluate the instrument's performance, I ran parallel glucose determinations in the Beckman Astra; results correlated well. The small sample size along with no dead sample volume makes the system useful for small sample volumes.
What preoperative factors predict postoperative sitting pelvic position one year following total hip arthroplasty? (2018)

Sitting lumbar-pelvic-femoral alignment following THA may be driven by hip arthritis and/or spinal deformity. Patients with DDD and fixed spinopelvic alignment have a predictable pelvic position one year following THA. Patients with normal spines have less predictable postoperative pelvic position, which is likely to be driven by hip stiffness. Cite this article: Bone Joint J 2018;100-B:1289-96.
Phase-Transfer Catalyst-Induced Changes in the Absorption and Fluorescence Behavior of Some Electron Donor−Acceptor Molecules (2001)

The quaternary ammonium salts find extensive use in a variety of chemical applications as surfactants and phase-transfer catalysts. Even though the interaction of the surfactants with various systems has been studied rather extensively, very little information is available on how the phase-transfer catalysts (PTC) interact with the dipolar systems in nonpolar media and how/whether these solubilizing agents affect the properties of the dissolved molecules. In this paper, the interaction of several tetraalkylammonium salts, commonly used as PTC, with a number of electron donor-acceptor (EDA) systems has been studied by absorption and fluorescence spectroscopy. The phase-transfer catalysts have been found to affect both the absorption and fluorescence properties of the EDA systems. The spectral changes of the EDA molecules induced by the tetraalkylammonium salts suggest the formation of an 1:1 complex between the two in nonpolar media. An electrostatic interaction between the phase-transfer catalysts (which exist as ion pairs in nonpolar media) and the dipolar molecules is shown to be the driving force for the formation of the complex. The dependence of the formation constant of the complex on the polarity of the media suggests a charge-transfer nature of the complex. It is shown that the anionic component of the salts serves as a source of electron to the positive end of the dipolar molecule, while the tetraalkylammonium cation, besides helping solubilization of its anionic counterpart in the nonpolar media, serves neutralizing the negative charge at the acceptor end of the EDA molecule. In effect, a cooperative influence of the cationic and anionic components of the PTC enhances the charge separation within the dipolar fluorophores. On the basis of the PTC-induced changes in the photophysical behavior of the EDA molecules, a possible structure for the 1:1 complex has been proposed. It has been concluded that a phase-transfer catalyst should not be treated as an innocuous substance that merely helps transfer of a polar substance from a polar to a nonpolar environment. Instead, it is demonstrated that the association of a PTC with a dipolar species can significantly change various properties of the latter.
Blind source separation of real world signals (2002)

We present a method to separate and deconvolve sources which have been recorded in real environments. The use of noncausal FIR filters allows us to deal with nonminimum mixing systems. The learning rules can be derived from different viewpoints such as information maximization, maximum likelihood and negentropy which result in similar rules for the weight update. We transform the learning rule into the frequency domain where the convolution and deconvolution property becomes a multiplication and division operation. In particular the FIR polynomial algebra techniques as used by Lambert present an efficient tool to solve true phase inverse systems allowing a simple implementation of noncausal filters. The significance of the methods is shown by the successful separation of two voices and separating a voice that has been recorded with loud music in the background. The recognition rate of an automatic speech recognition system is increased after separating the speech signals.
Diffusion‐weighted imaging of human carotid artery using 2D single‐shot interleaved multislice inner volume diffusion‐weighted echo planar imaging (2D ss‐IMIV‐DWEPI) at 3T: Diffusion measurement in atherosclerotic plaque (2009)

2D ss-IMIV-DWEPI can perform diffusion-weighted carotid magnetic resonance imaging (MRI) in vivo with reasonably high spatial resolution (1 x 1 x 2 mm(3)). ADC values of the carotid wall and plaque are consistent with similar values obtained from ex vivo endarterectomy specimens. The spread in ADC values obtained from plaque indicate that this technique could form a basis for plaque component identification in conjunction with other MRI/MRA techniques.
Computer signal processing of long duration biotelemetric brain data. (1975)

The EEG represents brain processing under diverse physiological conditions. A complete system involving acquisition and quantitation of this important information about brain function is described. The time-domain EEG and other biological signals are obtained using a multichannel PAM/FM biotelemeter mounted on the head of the experimental animal. This data is transmitted, demodulated and recorded by electronic recording techniques. A computer-based EEG analysis system is described for acquiring the primary data and transforming it into the frequency domain using Fourier methods. The computing system is developed to semi-automatically signal process about 4 h of eight channel EEG records. Data compression by plotting in a quasi-three-dimensional spectral profile allows visual correlations of pattern features to drug manipulations, etc. The software programs are briefly described for each step in signal processing. The feasibility of the complete system approach is demonstrated using biotelemetry to acquire low voltage EEG signals without behavioral distortions or introduction of artifacts by cables.