gebhart/patrepeval huggingface dataset:
Impaired germinal center formation and recall T-cell–dependent immune responses in mice lacking the costimulatory ligand B7-H2 (2003)

B7-H2, which is expressed constitutively on B cells and binds the inducible costimulator (ICOS) on antigen-activated T cells, is a member of the B7 family of costimulatory ligands. We have inactivated B7-H2 in the mouse. B7-H2-/- mice generate normal populations of B and T cells in their various lymphoid organs but have lower basal levels of heavy chain class-switched antibodies in their sera. These mice are able to mount normal immune responses to both type I and type II T-cell-independent antigens. However, their pattern of responses to a T-cell-dependent antigen is altered, with greatly reduced production of antigen-specific heavy chain class-switched antibodies, the levels of which could not be elevated even with repeated immunizations. This suggests a critical role for B7-H2 in the recall phases of the immune response. Germinal center formation is also impaired in the mutant mice. While B cells from the mutant mice could response normally to anti-IgM, anti-CD40, and lipopolysaccharide stimulation, the production of T-helper-type II cytokines such as interleukin-4 (IL-4) and IL-10 by primed CD4+ T cells from mutant mice were reduced. This indicated that the defects in humoral responses and germinal center formation in B7-H2-deficient mice are due to the lack of T-cell-mediated help to the B cells. Hence, B7-H2 on B cells is important for recruiting T-cell help via its interaction with ICOS and plays a critical role in costimulating humoral immune responses.
Unambiguous Synthesis and Prophylactic Antimalarial Activities of Imidazolidinedione Derivatives (2005)

WR182393, a guanidinoimidazolidinedione derivatives with potent causal prophylactic antimalarial activity by intramuscular injection, was previously prepared by treatment of chloroproguanil and diethyl oxalate, yielding a mixture of two closely related isomers. Poor solubility of the mixture made the separation and purification impossible. To overcome the separation problem, new and facile unambiguous syntheses of the two active components were reported. The new synthetic methods facilitate the synthesis of not only the active components, but also their derivatives. To search for compounds with good oral efficacy, a series of carbamate derivatives of the active components were prepared by the new procedure, many of which showed profound causal prophylactic antimalarial activity against Plasmodium yoelii in mouse by oral administration.
<title>Homodyne frequency-domain instrument: I &amp; Q Phase Detection System</title> (1997)

The noninvasive NIR detection technique can be used to detect the clinic-related parameter such as hemoglobin concentration and saturation. By now, many techniques have been demonstrated in both time-domain and frequency domain to obtain quantitative optical properties. In this paper, we demonstrate one kind of simple homodyne phase modulation system -- in- phase and quadrature phase (I&O) detection system, introducing its principle and constitution, presenting its performance through the tests of ink, intralipid and blood model.
A Criterion for Topological Close-Packed Phase Formation in High Entropy Alloys (2015)

The stability of topological close-packed (TCP) phases were found to be well related to the average value of the d-orbital energy level \( \overline{Md} \) for most reported high entropy alloys (HEAs). Excluding some HEAs that contain high levels of the elements aluminum and vanadium, the results of this study indicated that the TCP phases form at \( \overline{Md} \) > 1.09. This criterion, as a semi-empirical method, can play a key role in designing and preparing HEAs with high amounts of transitional elements.
High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi (2008)

We provide a new high-efficiency cloning system for Rosa26 knock-in constructs to express either cDNA or miRNA fragments. Our system will enable high-throughput approaches for controlled expression of cDNA or miRNA constructs, with the latter providing a potential high-speed alternative for conditional knock-out models.
Cardiac abnormalities in stroke patients with negative arteriograms. (1986)

Sixty-five consecutive patients with recent unequivocal TIA (33) or stroke (32), but nondiagnostic arteriograms, had two-dimensional echocardiograms (2DE) and electrocardiograms (ECG) to determine the incidence of cardiac abnormalities which could cause embolic stroke. Abnormalities were classified according to increasing probability of causing an embolic event: non-specific, possible emboligenic abnormality (PEA) or definite emboligenic abnormality (EA). Although 2DE was abnormal in 33 patients (51%), and ECG in 38 (59%), many abnormalities were nonspecific. Only four patients (6%) had EA on ECG and two (3%) on 2DE. Since one patient had EA on both tests, 2DE identified only one patient (mitral valve prolapse) not already identified by ECG. All patients with EA had a prior history of cardiac disease. PEA was present on ECG in 11 patients (17%), and on 2DE in 25 (38%). There was no correlation between age, CT results, or neurologic symptoms commonly associated with embolic stroke and the presence of EA or PEA on ECG or 2DE. Although TIA and stroke patients with negative arteriograms have a high incidence of abnormalities on ECG and 2DE, the percentage of patients with EA is low, and cardiac history and ECG identify most patients. 2DE provides little additional information.
Heterogeneity of Sindbis Virus Glycoprotein E1 and its Modification by Host Cell Transformation (1984)

The electrophoretic properties of glycoprotein E1 of Sindbis virus grown in Rous sarcoma virus-transformed cells were compared with those of Sindbis virus grown in untransformed cells. Isoelectric focusing in a gel containing 9.5 M-urea and 2% Nonidet P40 indicated that the glycoprotein E1 of Sindbis virus from the untransformed cells was electrochemically heterogeneous and consisted of four components, whose isoelectric points (pIs) ranged from 6.2 to 6.7; the E1 of Sindbis virus from the transformed cells contained an additional component with a pI of 6.1. After treatment with neuraminidase the observed charge heterogeneity disappeared regardless of whether the virus had come from the transformed or the untransformed cells. The five components were digested with Pronase and compared by gel filtration on Bio-Gel P-6; the four components with relatively higher pIs released high-mannose-type and complex-type oligosaccharides which differed in their content of sialic acid. In contrast, the pI 6.1 component, which existed only in the transformed cell-derived SV glycoprotein E1, released only complex-type oligosaccharide. These results indicate that the properties of glycoprotein E1 derived from both cell types are basically similar but that a small proportion of the E1 molecules from the transformed cells, with carbohydrate chains elongated by attachment of sialic acid residues, lacks simple-type oligosaccharides either because they are not attached or because they have been processed to the complex type. Glycoprotein E2 appeared as at least three components in non-equilibrium pH gradient electrophoresis. However, no significant difference was observed between the cell types.
Advanced glycation end-products prepared in solution under high pressure contain epitopes distinct from those formed in the dry reaction at high temperature. (2005)

The immunoblotting experiments showed that the epitopes on the cross-linked glycation products formed in solution under high pressure differed from those originating in dry conditions at high temperature. Sera against the HPG products were specific to homologous material and did not interact with the fractions obtained by HTG. The antibodies against HTG products recognized HTG but not HPG products.
Metabolic characteristics of oxcarbazepine (®trileptal) and their beneficial implications for enzyme induction and drug interactions. (1990)

Hepatic oxygenases of the cytochrome P-450 family play a major role in the clearance of various anti-epileptic drugs. These enzymes are susceptible both to induction and to inhibition. Phenytoin, carbamazepine (CBZ), primidone, and phenobarbitone, for instance, are potent enzyme inducers. Other drugs, such as chloramphenicol, propoxyphene, verapamil, and viloxazine, inhibit cytochrome P-450. Pharmacokinetic behaviour is thus often altered, especially in combined medication, so that the dosage has to be re-adjusted if an optimum therapeutic outcome is to be ensured.Oxcarbazepine (OXC) is a keto analogue of CBZ. In the human liver the keto group is readily reduced, and the resulting monohydroxy metabolite is cleared by glucuronidation. The two enzymes mediating these reactions, i.e. aldo-keto reductase and UDP-glucuronyltransferase, do not depend on cytochrome P-450. The monohydroxy metabolite is the major active substance in plasma. Its elimination is not enhanced by OXC. Moreover, OXC seems to have little effect on cytochrome P-450. Aldo-keto reductases and glucuronyltransferases are in general less sensitive to induction and inhibition than are P-450 dependent enzymes. On the whole, OXC possesses very little potential for metabolic drug interactions, and thus differs favourably from other anti-epileptic drugs.
Expression of TNF-<i>α</i>, April and BCMA in Behcet’s Disease (2014)

Patients have significantly higher levels of TNF family members' (TNF-α, BAFF, APRIL, and BCMA) compared to controls which might contribute to the pathogenesis of BD.
Mutations of the <i>UMOD</i> gene are responsible for medullary cystic kidney disease 2 and familial juvenile hyperuricaemic nephropathy (2002)

These data provide the first direct evidence that MCKD2 and FJHN arise from mutation of the UMOD gene and are allelic disorders. UMOD is a GPI anchored glycoprotein and the most abundant protein in normal urine. We postulate that mutation of UMOD disrupts the tertiary structure of UMOD and is responsible for the clinical changes of interstitial renal disease, polyuria, and hyperuricaemia found in MCKD2 and FJHN.
Temperature Monitoring Practices During Regional Anesthesia (1999)

The results of this survey of practicing anesthesiologists indicate that body temperature is often not monitored in patients receiving regional anesthesia. It is therefore likely that significant hypothermia goes undetected and untreated in these patients.
Reversible Insertion Properties of Zinc Ion into Manganese Dioxide and Its Application for Energy Storage (2009)

The reversible intercalation of ions into manganese dioxide was first reported in an aqueous system and a large capacity was measured. A cycle life test was performed at , and after , no capacity fading was found, which indicates the good cycling properties of manganese dioxide toward the insertion of zinc ions. X-ray photoelectron spectrum measurements indicated that ions in the electrolyte are involved in the charge storage process of manganese dioxide. X-ray diffraction results exhibited the kinetic stability of the host structure in allowing high-capacity, single-phase, and reversible zinc-ion intercalation.
Super-low-k SiOCH film (k = 1.9) with extremely high water resistance and thermal stability formed by neutral-beam-enhanced CVD (2010)

We developed a neutral-beam-enhanced method of chemical vapour deposition (NBECVD) to obtain a lower dielectric constant for the SiOCH interlayer dielectric film while maintaining a reasonable modulus. We achieved a higher deposition rate than that with the precursor of dimethyl-dimethoxy-silane (DMDMOS) we previously reported on by using Ar NBECVD with a precursor of dimethoxy-tetramethyl-disiloxine (DMOTMDS). This is because of the high absorption coefficient of DMOTMDS. Ar NBECVD with DMOTMDS also achieved a much lower dielectric constant than the conventional PECVD film, because this method avoids the precursor dissociation that causes low dielectric film with many linear Si–O structures. We obtained a k value of 1.9 for the super-low- k SiOCH film with an extremely water resistant, and very thermally stable and integration-possible modulus (>4 GPa) by controlling the bias power.
An 8W GaN-based H-bridge class-D PA for the 900 MHz band enabling ternary coding (2012)

This paper presents an H-bridge class-D power amplifier (PA) for the 900 MHz band based on GaN MMICs. For a classical BPDS modulated signal, a maximum output power of 34.5 dBm with a drain efficiency of 22% is obtained. Using a periodic square-wave input signal, the PA achieves a peak output power of 39 dBm and maximum drain efficiency of 48%. Investigating different binary and a ternary coding schemes, up to 30% drain efficiency at 6 dB power back-off is achieved.
Mechanical Properties of Thermomechanically-Processed Metastable Beta Ti-Nb-Zr Alloys for Biomedical Applications (2012)

Metastable beta-titanium alloys combine exceptionally low Young's modulus and high biocompatibility, thus attracting special interest in the prospect of their application as biomedical implant material. In this work, Ti-21.8Nb-6Zr (at.%) ingots were manufactured by vacuum argon melting followed by hot isothermal pressing. The obtained ingots were thermomechanically processed using the following TMP sequence: a) cold rolling (CR) from e=0.37 to 2 of the logarithmic thickness reduction; and b) post-deformation annealing (PDA) of between 450 and 700°C (10’…5 h for 600°C and 1 h for other temperatures). The influence of the TMP on the alloy’s mechanical properties under static and cyclic loading was studied.
Influence of lung volume in sleep apnoea. (1989)

The influence of a constant increase in functional residual capacity on apnoea characteristics was studied in patients with the sleep apnoea syndrome. Pulmonary inflation was achieved by applying a continuous negative extrathoracic pressure into a Poncho type respirator. Nine patients slept in the Poncho for two consecutive nights, negative extrathoracic pressure being applied during the second night. There was no difference in the total sleep time, its composition within the different sleep stages, the apnoea and apnoea-hypopnoea indices, or the sleep time spent in apnoea between the two nights. The mean (SD) apnoea duration increased with negative extrathoracic pressure from 25.3 (2) to 30.5 (3) seconds (p = 0.003) and time spent in obstructive apnoea (percentage of apnoea time) from 56 (13) to 75 (8) (p = 0.02). The mixed apnoea time (%) decreased from 37 (7) to 21 (7) (p = 0.02). Despite the increase in apnoea duration, less time was spent below each oxygen saturation value during negative extrathoracic pressure. The results were similar for apnoeic episodes during non-REM (non-rapid eye movement) sleep, whereas no significant modifications were seen during REM sleep. It is concluded that the composition of apnoea time and resulting oxygen desaturation are influenced by lung volume.
Modification of Sputter-Deposited Nanocrystalline Li[sub x]Mn[sub 2−y]O[sub 4] Thin-Film Cathodes by In Situ Substrate Bias and Postanneal (2005)

Thin films of lithium manganese oxides were deposited by radio frequency magnetron sputtering. Negative electric bias was applied on the substrates during deposition. The deposited films exhibited a defected spinel structure, , as characterized by X-ray diffraction and micro-Raman spectroscopy. As the substrate bias increased, the films developed stronger (111) orientation. At high substrate bias, the films tended to lose lithium and a second phase of manganese oxide developed. Postanneal was also carried out to fabricate well-crystallized spinel thin films for comparison. The film morphology also varied with substrate bias as observed by scanning electron microscope. By substrate bias and anneal, well-crystallized films with different morphologies can be obtained. Charge–discharge and cyclic voltammetry curves of these thin films were measured and compared. The different electrochemical characteristics of these films were attributed to the modified crystallography, morphology, and film stress. The films deposited with in situ substrate bias exhibited larger capacity without well-defined plateau. The postannealed films without substrate bias showed well-defined 4.1 and 3.9 V plateaus with slightly lower capacity. The biased and annealed films had low capacity but showed only 3.5% of capacity loss after 30 cycles.
Harvesting a clock from a GSM signal for the wake-up of a wireless sensor network (2010)

This paper presents a low-power technique for harvesting a clock from the Global System for Mobile Communications (GSM) cellular network for the purpose of waking up and synchronizing nodes in a wireless sensor network. The 21-Hz clock is embedded within the broadcast channel of every GSM cell worldwide, making it a pervasive synchronization source. A low-power receiver architecture is presented to harvest the signal. Based on simulation results, the architecture can extract the clock with an error probability of less than 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-4</sup> . Finally, a discrete prototype was built to verify the functionality of the receiver architecture.
Comparisons of neuronal (PGP 9.5) and non-neuronal ubiquitin <i>C</i>-terminal hydrolases (1992)

Conference Article| August 01 1992 Comparisons of neuronal (PGP 9.5) and non-neuronal ubiquitin C-terminal hydrolases Keith D. Wilkinson; Keith D. Wilkinson 1Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Seema Deshpande; Seema Deshpande 1Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Christopher N. Larsen Christopher N. Larsen 1Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1992) 20 (3): 631–637. https://doi.org/10.1042/bst0200631 Article history Received: April 16 1992 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Keith D. Wilkinson, Seema Deshpande, Christopher N. Larsen; Comparisons of neuronal (PGP 9.5) and non-neuronal ubiquitin C-terminal hydrolases. Biochem Soc Trans 1 August 1992; 20 (3): 631–637. doi: https://doi.org/10.1042/bst0200631 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: hsp, heat-shock protein, UCH, ubiquitin C-terminal hydrolase This content is only available as a PDF. © 1992 Biochemical Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
3D color object reconstruction from 2D image sequences (2003)

We have developed an automatic system for 3D color reconstruction of real objects from 2D image sequences captured with a calibrated camera. The surface model is carved in an octree structure by using a shape from silhouette approach. The resulting 3D triangle mesh is obtained by a marching cube technique followed by a mesh decimation. The color related tasks of the whole system, such as object extraction and texture mapping, are addressed in the paper. We present a texture mapping strategy based on surface particles, It improves the texture quality by proper selection of the original color images yielding smooth color texture transitions both at triangle boundaries and within triangles. A particular care has been taken for the highlight removal problem. Reconstruction results demonstrate the attained quality.
Detection of micrometastatic breast cancer by means of real time quantitative RT‐PCR and immunostaining in perioperative blood samples and sentinel nodes (2003)

The aim of our study was to detect micrometastatic breast cancer by epithelial glycoprotein-2 (EGP-2) and cytokeratin 19 (CK19), using immunostaining and real time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Fifty-eight breast cancer patients, 52 primary tumors, 75 sentinel nodes (SN) and 149 peripheral blood (PB) samples (from before, during and 4 days after operation) were examined. Immunostaining was performed with antibodies directed against EGP-2 and CK19. Detection limits were one Michigan Cancer Foundation-7 (MCF-7) breast cancer cell line cell/2.10(6) leukocytes (immunostaining) and one MCF-7 cell/10(6) leukocytes qRT-PCR. Control noncancer lymph nodes (n = 10) showed nonspecific CK19 staining, but were qRT-PCR negative; control healthy volunteer PB (n = 11) was always negative. Primary tumor samples, all positive with immunostaining, showed a wide variation of EGP-2 (>10(4) fold) and CK19 mRNA expression (>10(3) fold). SN (n = 19) from 16 patients were tumor-positive with routine haematoxylin-eosin (H&E) and/or immunostaining. SN tumor presence was positively correlated to qRT-PCR expression, but 3 tumor-positive SN were false negative with qRT-PCR. Three SN were qRT-PCR positive, while tumor negative with H&E and/or immunostaining. No immunostaining positive PB was observed, but 19 patients (33%) had one or more qRT-PCR positive PB samples. We concluded that primary tumors have varying expressions of EGP-2 and CK19 mRNA. Both markers can be used in qRT-PCR to obtain adequate sensitivity for single tumor cell detection. In SN, immunostaining appears more sensitive/specific than H&E or qRT-PCR for tumor detection. No immunostaining positivity was found in PB, while 33% of patients had qRT-PCR positive PB. The clinical value of these findings will have to be clarified.
Concise Review: Circulating Endothelial Progenitor Cells for Vascular Medicine (2011)

Endothelial progenitor cells (EPCs) have been isolated and shown to be effective in animal models of ischemia, and many groups involved in clinical trials have demonstrated that EPC therapy is safe and feasible for the treatment of critical limb ischemia and cardiovascular diseases. However, many issues in the field of EPC biology, especially in regards to the proper and unambiguous molecular characterization of these cells still remain unresolved, hampering not only basic research but also the effective therapeutic use and widespread application of these cells. In this review, we introduce the recent concept of EPC identification in terms of hematopoietic and nonhematopoietic EPCs along with the development of EPC biology research. Furthermore, we define the role of circulating EPCs in postnatal neovascularization to illustrate the future direction of EPC therapeutic applications. Next, we review on-going medical applications of EPC for cardiovascular and peripheral vascular diseases, introduce the practical example of therapeutic application of EPCs to patients with ischemic disease, and discuss about the feedback of clinical researches.