gebhart/patrepeval huggingface dataset:
Adenosine A2 Receptor Activation Attenuates Afferent Arteriolar Autoregulation During Adenosine Receptor Saturation in Rats (2007)

Adenosine is an important paracrine agent regulating renal hemodynamics via adenosine A1 and A2 receptors. To determine the interactions between adenosine A1 and A2 receptors and the possible role of adenosine as a modulator of afferent arteriolar autoregulatory responses, videomicroscopic measurements of afferent arteriolar dimensions were performed at different perfusion pressures (from 100 to 125 and 150 mm Hg) using the isolated-blood-perfused rat juxtamedullary nephron preparation. Single afferent arterioles were visualized and superfused with low or high concentrations of adenosine, either alone or with the adenosine A1 receptor antagonist 8-noradamantan-3-yl-1,3-dipropylxanthine (10 micromol/L) or the adenosine A2 receptor antagonist dimethyl-1-propargylxanthine (10 micromol/L). Adenosine (20 micromol/L) decreased afferent arteriolar diameter by -9.0+/-0.9%, and this effect was enhanced by dimethyl-1-propargylxanthine (10 micromol/L) to -16.1+/-1.2%. However, autoregulatory capability was maintained. Adenosine-induced vasoconstriction was prevented by 8-noradamantan-3-yl-1,3-dipropylxanthine (10 micromol/L) with diameter increasing by 9.6+/-1.2%. Adenosine receptor saturation with a high concentration of adenosine (120 micromol/L) or blocking A1 receptors with 8-noradamantan-3-yl-1,3-dipropylxanthine in the presence of adenosine resulted in marked vasodilation and marked impairment of autoregulatory responses to increases in perfusion pressure (-1.5+/-1.1% and -3.5+/-0.9%). However, afferent arteriolar autoregulatory responses to elevations in perfusion pressure were restored after blockade of A2 receptors alone or in combination with A1 receptor blockade. During treatment with dimethyl-1-propargylxanthine in the presence of adenosine receptor saturation (120 micromol/L), afferent arteriolar autoregulatory responses were intact (-16.5+/-1.6% and -26.4+/-2.1%). These results indicate that the interactions between adenosine A1 and A2 receptors exert important modulatory influences on afferent arteriolar tone and autoregulatory capability. Activation of A2 receptors abrogates the counteracting influences of A1 receptors leading to marked vasodilation and decreased afferent arteriolar autoregulatory efficiency.
6.3 A 0.5V, 400MHz, VDD-Hopping Processor with Zero-VTH FD-SOI Technology (2003)

Fully-depleted silicon-on-insulator (FD-SOI) technology is promising because of its scalability. A 0.5V 100MHz adder and SRAM in FD-SOI has been previously reported [1]. A much faster 0.5V 400MHz FD-SOI processor is presented with power consumption of the 3.5mW. High-speed operation is achieved with zero threshold voltage (VTH). To suppress leakage, memories accounting for 85% of transistor count are built with a higher VTH of 0.3V. A higher speed of 800MHz is achieved with a 0.9V supply voltage (VDD) through the VDD-hopping scheme [2], where the higher VDD is applied only when higher performance is needed. It is demonstrated that VDD-hopping is effective even in leakage dominant environments.
Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs (2012)

The bacteriophage T4-encoded RegB endoribonuclease is produced during the early stage of phage development and targets mostly (but not exclusively) the Shine-Dalgarno sequences of early genes. In this work, we show that the degradation of RegB-cleaved mRNAs depends on a functional T4 polynucleotide kinase/phosphatase (PNK). The 5'-OH produced by RegB cleavage is phosphorylated by the kinase activity of PNK. This modification allows host RNases G and E, with activity that is strongly stimulated by 5'-monophosphate termini, to attack mRNAs from the 5'-end, causing their destabilization. The PNK-dependent pathway of degradation becomes effective 5 min postinfection, consistent with our finding that several minutes are required for PNK to accumulate after infection. Our work emphasizes the importance of the nature of the 5' terminus for mRNA stability and depicts a pathway of mRNA degradation with 5'- to 3'-polarity in cells devoid of 5'-3' exonucleases. It also ascribes a role for T4 PNK during normal phage development.
Graphite Screen-Printed Electrodes Applied for the Accurate and Reagentless Sensing of pH (2015)

A reagentless pH sensor based upon disposable and economical graphite screen-printed electrodes (GSPEs) is demonstrated for the first time. The voltammetric pH sensor utilizes GSPEs which are chemically pretreated to form surface immobilized oxygenated species that, when their redox behavior is monitored, give a Nernstian response over a large pH range (1-13). An excellent experimental correlation is observed between the voltammetric potential and pH over the entire pH range of 1-13 providing a simple approach with which to monitor solution pH. Such a linear response over this dynamic pH range is not usually expected but rather deviation from linearity is encountered at alkaline pH values; absence of this has previously been attributed to a change in the pKa value of surface immobilized groups from that of solution phase species. This non-deviation, which is observed here in the case of our facile produced reagentless pH sensor and also reported in the literature for pH sensitive compounds immobilized upon carbon electrodes/surfaces, where a linear response is observed over the entire pH range, is explained alternatively for the first time. The performance of the GSPE pH sensor is also directly compared with a glass pH probe and applied to the measurement of pH in "real" unbuffered samples where an excellent correlation between the two protocols is observed validating the proposed GSPE pH sensor.
Activation of a Recombinant Petunia Glutamate Decarboxylase by Calcium/Calmodulin or by a Monoclonal Antibody Which Recognizes the Calmodulin Binding Domain (1996)

To date, only plants have been shown to possess a form of glutamate decarboxylase (GAD) that binds calmodulin. In the present study, a recombinant calmodulin-binding 58-kDa petunia GAD produced in Escherichia coli was purified to homogeneity using calmodulin-affinity chromatography, and its responsiveness to calcium and calmodulin was examined in vitro. At pH 7.0-7.5, the purified recombinant enzyme was essentially inactive in the absence of calcium and calmodulin, but it could be stimulated to high levels of activity (Vmax = 30 micromol of CO2 min-1 mg of protein-1) by the addition of exogenous calmodulin (K0.5 = 15 nM) in the presence of calcium (K0.5 = 0.8 microM). Neither calcium nor calmodulin alone had any effect on GAD activity. Recombinant GAD displayed hyperbolic kinetics at pH 7.3 (Km = 8.2 mM). A monoclonal antibody directed against the carboxyl-terminal region, which contains the calmodulin-binding domain of GAD, was able to fully activate GAD in a dose-dependent manner in the absence of calcium and calmodulin, whereas an antibody recognizing an epitope outside of this region was unable to activate GAD. This study provides the first evidence that the activity of the purified 58-kDa GAD polypeptide is essentially calcium/calmodulin-dependent at physiological pH. Furthermore, activation of GAD by two different proteins that interact with the calmodulin-binding domain, a monoclonal antibody or calcium/calmodulin, suggests that this domain plays a major role in the regulation of plant GAD activity.
Translucent shadow maps (2003)

Shadow maps are a very efficient means to add shadows to arbitrary scenes. In this paper, we introduce Translucent Shadow Maps, an extension to shadow maps which allows very efficient rendering of sub-surface scattering. Translucent Shadow Maps contain depth and incident light information. Sub-surface scattering is computed on-the-fly during rendering by filtering the shadow map neighborhood. This filtering is done efficiently using a hierarchical approach. We describe optimizations for an implementation of Translucent Shadow Maps on contemporary graphics hardware, that can render complex translucent objects with varying light and material properties in real-time.
The <i>txtAB</i> genes of the plant pathogen <i>Streptomyces acidiscabies</i> encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity (2000)

Four Streptomyces species have been described as the causal agents of scab disease, which affects economically important root and tuber crops worldwide. These species produce a family of cyclic dipeptides, the thaxtomins, which alone mimic disease symptomatology. Structural considerations suggest that thaxtomins are synthesized non-ribosomally. Degenerate oligonucleotide primers were used to amplify conserved portions of the acyladenylation module of peptide synthetase genes from genomic DNA of representatives of the four species. Pairwise Southern hybridizations identified a peptide synthetase acyladenylation module conserved among three species. The complete nucleotide sequences of two peptide synthetase genes (txtAB) were determined from S. acidiscabies 84.104 cosmid library clones. The organization of the deduced TxtA and TxtB peptide synthetase catalytic domains is consistent with the formation of N-methylated cyclic dipeptides such as thaxtomins. Based on high-performance liquid chromatography (HPLC) analysis, thaxtomin A production was abolished in txtA gene disruption mutants. Although the growth and morphological characteristics of the mutants were identical to those of the parent strain, txtA mutants were avirulent on potato tubers. Moreover, introduction of the thaxtomin synthetase cosmid into a txtA mutant restored both pathogenicity and thaxtomin A production, demonstrating a critical role for thaxtomins in pathogenesis.
Nonhydrolytic Sol−Gel Routes to Oxides (1997)

This review surveys the chemistry of various nonhydrolytic sol−gel processes including hydroxylation in nonaqueous systems and aprotic condensation reactions. Some examples of applications in the field of single and multicomponent oxides demonstrate the interest of these approaches as alternatives to the usual hydrolytic routes. More particularly nonhydrolytic methods lead to improved control over the molecular level homogeneity and stoichiometry of multicomponent oxides.
Solventless Synthesis of Monodisperse Cu<sub>2</sub>S Nanorods, Nanodisks, and Nanoplatelets (2003)

Cu(2)S nanocrystals with disklike morphologies were synthesized by the solventless thermolysis of a copper alkylthiolate molecular precursor. The nanodisks ranged from circular to hexagonal prisms from 3 to 150 nm in diameter and 3 to 12 nm in thickness depending on the growth conditions. High resolution transmission electron microscopy (HRTEM) revealed the high chalcocite (hexagonal) crystal structure oriented with the c-axis ([001] direction) orthogonal to the favored growth direction. This disk morphology is thermodynamically favored as it allows the extension of the higher energy [100] and [110] surfaces with respect to the [001] planes. The hexagonal prism morphology also appears to relate to increased C-S bond cleavage of adsorbed dodecanethiol along the more energetic [100] facets relative to [001] facets. Monodisperse Cu(2)S nanodisks self-assemble into ribbons of stacked platelets. This solventless approach provides a new technique to synthesize anisotropic metal chalcogenide nanostructures with shapes that depend on both the face-sensitive thermodynamic surface energy and the surface reactivity.
Automated antenna positioning for wireless networks (2006)

This article addresses a real-life problem - obtaining communication links between multiple base stations sites, by positioning a minimal set of fixed-access relay antenna sites on a given terrain. Reducing the number of relay antenna sites is considered critical due to substantial installation and maintenance costs. Despite the potential significant cost saving by eliminating even a single antenna site, a hardly optimal manual approach is employed due to the computation complexity of the problem. We suggest several alternative automated heuristics, relying on terrain preprocessing to find educated potential points for positioning relay stations. A large-scale experiment was conducted showing that the saving potential increases when more BSs are required to be interconnected and in any case is better than the one obtained by ah uman expert.
A coded 8‐PSK system for 140 Mb/s information rate transmission over 80 MHz non‐linear transponders (1986)

Abstract A combined 8‐PSK modulation and rate 7/9 convolutional coding technique is proposed for 140 Mb/s information rate transmission over the 80 MHz INTELSAT transponders, thus achieving a bandwidth efficiency of 1.75 b/s/Hz of allocated bandwidth. The desired power efficiency is to achieve a bit error rate of 10 −6 at an E b / N 0 of 11 dB, including modem and codec implementation losses. The proposed system employs an 8‐PSK modem operating at a 60 MHz symbol rate (or 180 Mb/s bit rate), as well as a rate 7/9 convolutional encoder and a 16‐state Viterbi algorithm decoder operating at 60 MHz. The rate 7/9 code is periodically time varying and is designed to maximize the Euclidean distance between the modulated codeword sequences, thereby achieving a 3 dB asymptotic coding gain relative to the conventional QPSK system over an AWGN channel. This code is also designed to reduce decoder complexity for high‐speed operations. The performance of the proposed system over INTELSAT V and VI non‐linear transponders was evaluated by Monte Carlo computer simulation. The 180 Mb/s 8 PSK modem, including the automatic frequency control, automatic gain control, carrier recovery and clock recovery circuits, has been implemented and tested. The complete Viterbi decoder is being implemented on five boards, and the critical add‐compare‐select (ACS) circuit of the high‐speed Viterbi algorithm decoder is being implemented with hybrid technology employing 100‐K series emitter‐coupled logic dies on specially designed ceramic substrates. The ACS circuit operates at a speed exceeding 120 MHz, well over the design goal of 60 MHz. Construction of this codec is almost complete.
Inflammatory cytokines induce AIDS-Kaposi's sarcoma-derived spindle cells to produce and release basic fibroblast growth factor and enhance Kaposi's sarcoma-like lesion formation in nude mice. (1995)

Homosexual males often present signs of immune activation and are likely to have increased levels of inflammatory cytokines such as IL-1 beta, TNF-alpha, and IFN-gamma. These individuals develop Kaposi's sarcoma (AIDS-KS) more frequently than other HIV-1-infected groups. Our previous work demonstrated that inflammatory cytokines stimulate the growth of spindle cells derived from AIDS-KS lesions (AIDS-KS cells) and that these cells produce high levels of bFGF that mediate autocrine and paracrine (endothelial) cell growth and angiogenesis. Here we show that AIDS-KS cells constitutively produce and release bioactive bFGF in the absence of cell death, and that extracellular bFGF exist in both a soluble and a bound form; the latter can be released by treatment with trypsin, heparin, or heparinase I. Inflammatory cytokines stimulate both the synthesis and release of biologically active bFGF from KS cells and enhance their ability to induce angiogenic KS-like lesions in nude mice. Because bFGF is highly expressed in primary KS lesions, and is a mediator of KS-like lesion formation, these results suggest that the export of bFGF induced by inflammatory cytokines may play a critical role in the induction and progression of KS in HIV-1-infected homosexual men.
Single-frequency thulium-doped silica DFB fiber laser at 1735 nm (2004)

We have developed a single-frequency thulium doped silica fiber laser with a Distributed FeedBack (DFB) cavity operating at a wavelength of 1735 <i>nm</i>. The laser cavity is 5 <i>cm</i> long formed by a UV-written Bragg grating with a phase shift and is pumped by a Ti:Sapphire laser at 790 <i>nm</i>. The laser operates in a single-polarization mode and is tunable over a few nanometers. To the best of our knowledge, this represents the first short cavity, single frequency fiber laser using thulium as the amplifying medium. The lasing wavelength is among the lowest demonstrated in a thulium-doped fiber laser and it falls in an attractive near-to-mid infrared wavelength region only offered by few sources. Single-frequency DFB fiber lasers are compact and stable optical sources, which offer low-noise coherent output with ultra-narrow-linewidth. Typical applications for DFB fiber lasers are as sources for coherent sensing, spectroscopy and several high-end applications. Using optical fiber doped with erbium and/or ytterbium these sources provide emission within the wavelength bands of 980 - 1200 <i>nm</i> and 1525 - 1620 <i>nm</i>. A thulium doped DFB laser opens a new broad wavelength range from 1.7 <i>μm </i>- 2.0 <i>μm</i>, depending on co-dopants. This wavelength range is especially interesting for use in gas sensors, frequency mixing and as a source for eye-safe LIDAR applications.
Simple and Rapid Method for the Selective Detection of Individual Lipoxygenase Isoenzymes in Soybean Seeds (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSimple and Rapid Method for the Selective Detection of Individual Lipoxygenase Isoenzymes in Soybean SeedsIkuo Suda, Makita Hajika, Yoichi Nishiba, Shu Furuta, and Kazunori IgitaCite this: J. Agric. Food Chem. 1995, 43, 3, 742–747Publication Date (Print):March 1, 1995Publication History Published online1 May 2002Published inissue 1 March 1995https://pubs.acs.org/doi/10.1021/jf00051a034https://doi.org/10.1021/jf00051a034research-articleACS PublicationsRequest reuse permissionsArticle Views534Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Connecting the Dots: Artificial Antigen Presenting Cell-Mediated Modulation of Natural Killer T Cells (2012)

Natural killer T (NKT) cells constitute an important subset of T cells that can both directly and indirectly mediate antitumor immunity. However, we and others have reported that cancer patients have a reduction in both NKT cell number and function. NKT cells can be stimulated and expanded with α-GalCer and cytokines and these expanded NKT cells retain their phenotype, remain responsive to antigenic stimulation, and display cytotoxic function against tumor cell lines. These data strongly favor the use of ex vivo expanded NKT cells in adoptive immunotherapy. NKT cell based-immunotherapy has been limited by the use of autologous antigen-presenting cells, which can vary substantially in their quantity and quality. A standardized system that relies on artificial antigen-presenting cells (aAPCs) could produce the stimulating effects of dendritic cell (DC) without the pitfalls of allo- or xenogeneic cells. In this review, we discuss the progress that has been made using CD1d-based aAPC and how this acellular antigen presenting system can be used in the future to enhance our understanding of NKT cell biology and to develop NKT cell-specific adoptive immunotherapeutic strategies.
Integration of Diverse Research Methods to Analyze and Engineer Ca2+- Binding Proteins: From Prediction to Production (2010)

In recent years, increasingly sophisticated computational and bioinformatics tools have evolved for the analyses of protein structure, function, ligand interactions, modeling and energetics. This includes the development of algorithms to recursively evaluate side-chain rotamer permutations, identify regions in a 3D structure that meet some set of search parameters, calculate and minimize energy values, and provide high-resolution visual tools for theoretical modeling. Here we discuss the interdependency between different areas of bioinformatics, the evolution of different algorithm design approaches, and finally the transition from theoretical models to real-world design and application as they relate to Ca(2+)-binding proteins. Within this context, it has become evident that significant pre-experimental design and calculations can be modeled through computational methods, thus eliminating potentially unproductive research and increasing our confidence in the correlation between real and theoretical models. Moving from prediction to production, it is anticipated that bioinformatics tools will play an increasingly significant role in research and development, improving our ability to both understand the physiological roles of Ca(2+) and other metals and to extend that knowledge to the design of function-specific synthetic proteins capable of fulfilling different roles in medical diagnostics and therapeutics.
Local Irritating Effect Caused by Topical Use of Steroids in the Eye (1957)

About a half a decade has elapsed since the introduction of the therapeutic use of corticosteroids. Experience has been accumulated as to their effectiveness in various ophthalmic diseases by systemic as well as by topical administration (Gordon, 1955<sup>16</sup>; Hogan, 1955<sup>8</sup>; Steffenson, 1955<sup>9</sup>). The splendid therapeutic responses have over-shadowed the local irritating effects of such topical preparations. Reports of such undesirable local side-effects are very brief, rare, and contradictory. <h3>Previous Reports</h3> The following citations from the literature will illustrate this point. <h3>A. Older Reports Dealing with Cortisone.</h3> —Woods, 1950<sup>1</sup>: A 2.5% suspension without buffer "caused mild transient irritation which passed in a few minutes." Woods, 1951<sup>2</sup>: "This suspension" (in buffered vehicle with added preservative) "is completely non-irritating, in fact is somewhat soothing to an inflamed eye." Duke-Elder, 1951<sup>3</sup>: "This is completely non-irritating, even if the undiluted suspension is used." Leopold et al., 1951<sup>4</sup>: "There