gebhart/patrepeval huggingface dataset:
Anti-EGFR-Conjugated Hollow Gold Nanospheres Enhance Radiocytotoxic Targeting of Cervical Cancer at Megavoltage Radiation Energies (2015)

The study aimed to confirm that anti-epidermal growth factor receptor (EGFR) monoclonal antibody-conjugated hollow gold nanospheres (anti-EGFR/HGNs) can be selectively uptaken by cervical cancer cells and induce its apoptosis when combined with radiotherapy, as a result enhancing radiosensitivity of cervical cancer cells. HGNs with a mean diameter of 54.6 ± 7.11 nm and wall thickness of 5.01 ± 2.23 nm were viewed by transmission electron microscopy (TEM). Cell uptake was assayed by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The cytotoxicity on HeLa cells, which were used in our experiment, was assessed by CCK-8 assay. Cell cycle and apoptosis were examined by an Annexin V-FITC/propidium iodide (PI) kit with flow cytometry (FCM). The expression of several critical apoptosis-related proteins, including Bcl-2, Bax, Bad, and active caspase 3, was tested by western blot analysis. Cells treated by anti-EGFR/HGNs showed an obvious increase in nanoparticle uptake compared to naked HGNs. Anti-EGFR/HGNs combined with radiation resulted in a significant growth inhibition, compared with radiation combined with naked HGNs. Anti-EGFR/HGNs remarkably increased the ratio of HeLa cells in the G2/M phase and induced more apoptosis by an obvious deregulation of Bcl-2 and upregulation of Bax, Bad, and caspase 3 when combined with radiation. Therefore, anti-EGFR/HGNs can increase the targeted uptake of HGNs by HeLa cells and enhance radiocytotoxic targeting of cervical cancer at megavoltage radiation energies.
Analysis of Buccolingual Dimensional Changes of the Extraction Socket Using the “Ice Cream Cone” Flapless Grafting Technique (2014)

Multiple adjacent recession defects were treated in 32 patients using a coronally advanced flap (CAF) with or without a collagen matrix (CM). The percentage of root coverage was 81.49% ± 23.45% (58% complete root coverage) for CAF sites (control) and 93.25% ± 10.01% root coverage (72% complete root coverage) for CM plus CAF sites (test). The results achieved in the test group were significantly greater than in the control group, indicating that CM plus CAF is a suitable option for the treatment of multiple adjacent gingival recessions.
Performance Assessment of Wireless Sensor Networks in Agricultural Settings (2007)

Wireless Sensor Network (WSN) utilizes radios operating primarily in the 900 MHz and 2.4 GHz frequency bands. In general, as frequency increases, bandwidth increases allowing for higher data rates but power requirements are also higher and transmission distance is considerably shorter. In general, depending on the operating environment, significant signal loss can occur at these frequencies particularly when the radios require line-of-sight for optimal performance, with 2.4 GHz more susceptible than 900 MHz. For agricultural applications, WSN must be able to operate in a range of environments, from bare fields to orchards, from flat to complex topography, and over a range of weather conditions, all of which affect radio performance. However, there are limited data on radio performance as affected by agricultural setting and no standard tests are available for quantifying WSN performance in agricultural applications. Using a low powered, 10 mW 900 MHz frequency hopping spread spectrum radio, we developed a range of tests intended to quantify the performance of agricultural WSN in fields, vineyards, and orchards over a range of crop and weather conditions. Performance data include different metrics of radio performance such as packet delivery and signal strength along with power consumption tests under different supply strategies. This paper evaluates the extent to which various tests can be used to quantify WSN performance and how WSN perform under various cropping systems.
SBMLmerge, a system for combining biochemical network models. (2006)

The Systems Biology Markup Language (SBML) is an XML-based format for representing mathematical models of biochemical reaction networks, and it is likely to become a main standard in the systems biology community. As published mathematical models in cell biology are growing in number and size, modular modelling approaches will gain additional importance. The main issue to be addressed in computer-assisted model combination is the specification and handling of model semantics. The software SBMLmerge assists the user in combining models of biological subsystems to larger biochemical networks. First, the program helps the user in annotating all model elements with unique identifiers pointing to databases such as KEGG or Gene Ontology. Second, during merging, SBMLmerge detects and resolves various syntactic and semantic problems. Typical problems are conflicting variable names, elements which appear in more than one input model, and mathematical problems arising from the combination of equations. If the input models make contradicting statements about a biochemical quantity, the user is asked to choose between them. In the end the merging process results in a new, valid SBML model.
Uropathogenic <i>Escherichia coli</i> Block MyD88-Dependent and Activate MyD88-Independent Signaling Pathways in Rat Testicular Cells (2008)

Uropathogenic Escherichia coli (UPEC) is the most common etiological cause of urogenital tract infections and represents a considerable cause of immunological male infertility. We examined TLR 1-11 expression profiles in testicular cells and the functional response to infection with UPEC. All testicular cell types expressed mRNAs for at least two TLRs and, in particular, synthesis of TLR4 was induced in testicular macrophages (TM), Sertoli cells (SC), peritubular cells (PTC), and peritoneal macrophages (PM) after UPEC exposure. Even though MyD88-dependent pathways were activated as exemplified by phosphorylation of mitogen-activated protein kinases in TM, SC, PTC, and PM and by the degradation of IkappaBalpha and the nuclear translocation of NF-kappaB in PTC and PM, treatment with UPEC did not result in secretion of the proinflammatory cytokines IL-1alpha, IL-6, and TNF-alpha in any of the investigated cells. Moreover, stimulated production of these cytokines by nonpathogenic commensal E. coli or LPS in PM was completely abolished after coincubation with UPEC. Instead, in SC, PTC, TM, and PM, UPEC exposure resulted in activation of MyD88-independent signaling as documented by nuclear transfer of IFN-related factor-3 and elevated expression of type I IFNs alpha and beta, IFN-gamma-inducible protein 10, MCP-1, and RANTES. We conclude that in this in vitro model UPEC can actively suppress MyD88-dependent signaling at different levels to prevent proinflammatory cytokine secretion by testicular cells. Thus, testicular innate immune defense is shifted to an antiviral-like MyD88-independent response.
Semimechanistic Pharmacokinetic/Pharmacodynamic Model for Assessment of Activity of Antibacterial Agents from Time-Kill Curve Experiments (2006)

Dosing of antibacterial agents is generally based on point estimates of the effect, even though bacteria exposed to antibiotics show complex kinetic behaviors. The use of the whole time course of the observed effects would be more advantageous. The aim of the present study was to develop a semimechanistic pharmacokinetic (PK)/pharmacodynamic (PD) model characterizing the events seen in a bacterial system when it is exposed to antibacterial agents with different mechanisms of action. Time-kill curve experiments were performed with a strain of Streptococcus pyogenes exposed to a wide range of concentrations of the following antibiotics: benzylpenicillin, cefuroxime, erythromycin, moxifloxacin, and vancomycin. Bacterial counts were monitored with frequent sampling during the experiment. A simultaneous fit of all data was accomplished. The degradation of the drugs was monitored and corrected for in the model, and a link model was used to account for an effect delay. In the final PK/PD model, the total bacterial population was divided into two subpopulations: one growing drug-susceptible population and one resting insusceptible population. The drug effect was included as an increase of the killing rate of bacteria in the susceptible state, according to a maximum-effect (E(max)) model. An internal model validation showed that the model was robust and had good predictability. In conclusion, for all drugs, the final PK/PD model successfully described bacterial growth and killing kinetics when the bacteria were exposed to different antibiotic concentrations. The semimechanistic model that was developed might, after further refinement, serve as a tool for the development of optimal dosing strategies for antibacterial agents.
Kinetics and kinematics of the equine hind limb: In vivo tendon strain and joint kinematics (1988)

Strains of the suspensory ligament and deep digital flexor, superficial digital flexor, and long digital extensor tendons in the equine (pony) hind limb were recorded in vivo, using implanted strain gauges consisting of silicone rubber tubes filled with mercury. The relationship between strain gauge signals and tendon strains was obtained from tension-strain tests performed on isolated tendons after death of the ponies. During normal walking, maximal tendon strain (elongation over initial length, relative to the length of the structures at first ground contact) was 3.1% in the suspensory ligament and 3.4%, 2.3%, and 0.3% in the deep digital flexor, the superficial digital flexor, and the long digital extensor tendons, respectively. Changes (that occurred during walking) in the distance from origin to insertion of these musculotendinous structures were computed from limb geometric configuration and limb conformation. Maximal increase in origin to insertion length was 3.1% in the suspensory ligament and 2%, 1.6%, and 1.5% in the deep digital flexor, superficial digital flexor, and long digital extensor musculotendinous structures, respectively. The differences in strain, comparing the entire musculotendinous structure and its tendon, were explained by muscular contraction or relaxation.
User Intention-Based Traffic Dependence Analysis for Anomaly Detection (2012)

This paper describes an approach to enforce dependencies between network traffic and user activities for anomaly detection. We present a framework and algorithms that analyze user actions and network events on a host according to their dependencies. Discovering these relations is useful in identifying anomalous events on a host that are caused by software flaws or malicious code. To demonstrate the feasibility of user intention-based traffic dependence analysis, we implement a prototype called CR-Miner and perform extensive experimental evaluation of the accuracy, security, and efficiency of our algorithm. The results show that our algorithm can identify user intention-based traffic dependence with high accuracy (average 99:6% for 20 users) and low false alarms. Our prototype can successfully detect several pieces of HTTP-based real-world spy ware. Our dependence analysis is fast with a minimal storage requirement. We give a thorough analysis on the security and robustness of the user intention-based traffic dependence approach.
Die Sekretärin im Management Rückblick — Standortbestimmung — Ausblick (1989)

In der Diskussion um eine zeitgemäße Führungsordnung in Wirtschaft und Verwaltung spielt auch die Frage nach Stellung und Funktion der Sekretärin im Management eine wichtige Rolle. Es besteht kein Zweifel daran, daß die Aufgaben der Führungsspitze wie der Führungskräfte auf den nachgeordneten Ebenen in den letzten Jahren immer komplizierter und vielseitiger geworden sind. Mit dieser Steigerung der Anforderungen an das Management haben sich zugleich auch die Anforderungen an die qualifizierte Sekretärin erhöht, die in diesem Bereich tätig ist. Die Funktionen, die die Sekretärin als Gehilfin des Chefs gegenüber seinen Mitarbeitern und anderen Stellen im Unternehmen zu erfüllen hat, haben sich wesentlich verändert. Über die Konsequenzen, die sich daraus für die organisatorische Einordnung des Sekretariats ebenso wie für die Regelung des Zusammenspiels zwischen Chef und Sekretärin ergeben, ist man sich jedoch keineswegs einig. Deshalb will auch die Diskussion um die Rolle der Sekretärin und ihre Zukunftsaussichten nicht abreißen. Die Sekretärin ist und bleibt ein immer wieder attraktives Thema für Soziologen, Politologen, Organisatoren, aber auch für die Technologen, die Rationalisierungsspezialisten und natürlich die Chefs selbst, mögen sie männlich oder weiblich sein.
Expression of the Gene for Ornithine Decarboxylase of <i>Saccharomyces cerevisiae</i> in <i>Escherichia coli</i> (1985)

Diploid cells of Saccharomyces cerevisiae homozygous for the spe1A mutation, which eliminates ornithine decarboxylase activity, were found to sporulate at a greatly reduced frequency in the absence of polyamines. Plasmids which complement the spe1A mutation were isolated by their ability to restore sporulation competence to these cells. Three distinct plasmids were isolated. Each plasmid insert overlapped the same 8.0-kilobase region, and each plasmid restored ornithine decarboxylase activity to spe1A mutants. These plasmids also conferred ornithine decarboxylase activity to Escherichia coli EWH319 from which the ornithine decarboxylase gene is deleted. The plasmid-encoded activity expressed in E. coli resembled S. cerevisiae ornithine decarboxylase in its kinetic characteristics, indicating that the yeast ornithine decarboxylase gene was cloned. Southern blot analysis suggested that ornithine decarboxylase is a single-copy gene in S. cerevisiae. A single 2.1-kilobase transcript was demonstrated by Northern blot analysis.
Wavefield Signal Apparition, Part I - Theory (2016)

Summary A new method for discrete sampling of signals with applications to separation of interfering wavefields is presented. By utilizing a periodic sequence of source signatures along one source line, the wavefield becomes separately visible in different parts of the spectral domain where it can be isolated from interfering signals, processed, and subtracted from the original recordings, thereby separating the wavefields from each other. The concept is referred to as signal apparition which we suggest refers to "the act of becoming visible". We anticipate a broad range of applications of the new sampling approach including simultaneous source separation, seismic interference cancellation, residual shot noise attenuation, modeling of wavefields and source-side deghosting. We illustrate the concept of seismic apparition on 2D finite-difference modeled data to remove the source ghost. In a companion paper (Sjøen Pedersen, Å., Amundsen, T., and Robertsson, J. O. A., 2016, Wavefield signal apparition, Part II: Application to simultaneous sources and their separation: Expanded abstract submitted to the EAGE annual meeting) we discuss the application of seismic apparition to simultaneous source acquisition to increase productivity and/or obtain a better source-side sampling of the seismic data.
Dietary fiber for cats: in vitro fermentation of selected fiber sources by cat fecal inoculum and in vivo utilization of diets containing selected fiber sources and their blends (1995)

Two experiments were conducted to evaluate the addition of single sources and blends of dietary fibers to cat diets. In Exp. 1, fermentability of selected fibrous substrates by cat fecal microflora was evaluated. After 24 h of fermentation, OM disappearance (OMD) and total short-chain fatty acid (SCFA) production were greatest (P < .05) for citrus pectin, guar gum, and locust bean gum, whereas Solka Floc resulted in the least (P < .05) OMD and total SCFA production. In Exp. 2, six diets were formulated based on results of Exp. 1. The highest (P < .05) digestibilities of DM and OM occurred when cats consumed the diet with no supplemental fiber, and the lowest (P < .05) digestibilities occurred when cats consumed the SCFA blend (SC) diet. Nitrogen and lipid digestibilities also were lowest (P < .05) for cats consuming the SC diet, whereas total dietary fiber (TDF) digestibility (P < .05) was greatest for cats consuming the beet pulp, SC, and combination blend diets. Fecal consistency scores were highest (P < .05) for cats consuming the SC diet, indicating liquid, unformed stools. In conclusion, the in vitro fermentation technique was reasonably accurate in predicting in vivo digestion of fiber. The SC diet, which contained the most fermentable fibers, severely decreased nutrient digestibility and resulted in poor stool characteristics. Diets that contain moderately fermentable fiber provide fermentation end products that may be important in maintaining the health of the gastrointestinal tract of the cat.
Estimation of O<sub>2</sub> and CO<sub>2</sub> Solubility in Microbial Culture Media (1999)

A simple model for predicting gas solubilities of O(2) and CO(2) at low pressure and near ambient temperature in solutions of salts, sugars, and organic solvents (alcohols, ketones, ethers, aldehydes, etc.) is proposed. It is derived from the Van Laar assumptions and takes account of size differences between molecules in solution by their volumetric fraction. It is a group contribution model where anions and cations are considered as groups and other molecules are treated as in the UNIFAC (UNIQUAC Functional group Activity Coefficients) procedure. Pseudo-Henry's constants for groups were determined using solubility data in aqueous solutions containing only one salt, one sugar, or one organic compound at 25 degrees C. The predictive performance of the model was evaluated by comparison with experimental data using multicomponent aqueous salt-sugar mixtures. The model was used to estimate solubilities of oxygen and carbon dioxide in fermentation media.
THE PREPARATIVE ISOLATION OF IMAGINAL DISCS FROM LARVAE OF <i>DROSOPHILA MELANOGASTER </i> (1965)

Imaginal discs of insccts have long been known to be embryonic Anlagen (1). Indeed, in Drosophila, the external structures in the head and thoracic regions, as well as some in the abdominal region, develop from imaginal discs (2). Discs develop normally when transplanted into larvae, and Schneider (3) has recently shown that eye discs develop normally and become pigmented in a defined medium. Thus, for the study of morphogenesis, imaginal discs offer a convenient tool. However, such studies have previously been hampered by the requirement for tedious manual dissection of discs. The present paper outlines a procedure for isolating large numbers of discs without dissection.
Conformational studies of some potentially bidentate thiosemicarbazones and related complexes of zinc(II) (1976)

N.m.r. spectra of some potentially bidentate thiosemicarbazones, NR3H·CS·NH·N:CR2R1(L: R1,R2= H, Me, Ph, or R1R2= C6H11; R3= H or Ph), and their complexes [ZnCl2L] or [ZnCl2L2] have been measured. The results are compared with semiempirical CNDO/2 total-energy calculations. Only one conformer, containing an intramolecular hydrogen bond, is present in solution. All the zinc(II) complexes are tetrahedral. The possible formation of complexes in which the ligand is bidentate is discussed.
Glutamate-like immunoreactivity in medulla oblongata catecholamine/substance P neurons (1990)

Immunofluorescence histochemistry was performed on sections of the rat medulla oblongata with a well characterized antibody to the amino acid glutamate (GLU) combined with antisera to catecholamine synthesizing enzymes and substance P. GLU-like immunoreactive (LIR) neurons were seen in many areas of the medulla, and were particularly intensely stained in the rostral ventrolateral medulla. In this area, nearly all adrenaline neurons were GLU-LIR. This immunoreactivity was also seen in catecholamine neurons of the C2, C3, A1 and A2 cell groups. Many adrenaline neurons, especially of the C1 group, contained substance P-LI in addition to GLU-like immunoreactivity (LI).
Role of ferryl (FeO2+) complexes in oxygen atom transfer reactions. Mechanism of iron(II) porphyrin catalyzed oxygenation of triphenylphosphine (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRole of ferryl (FeO2+) complexes in oxygen atom transfer reactions. Mechanism of iron(II) porphyrin catalyzed oxygenation of triphenylphosphineDer-Hang Chin, Gerd N. La Mar, and Alan L. BalchCite this: J. Am. Chem. Soc. 1980, 102, 18, 5945–5947Publication Date (Print):August 1, 1980Publication History Published online1 May 2002Published inissue 1 August 1980https://doi.org/10.1021/ja00538a059RIGHTS & PERMISSIONSArticle Views566Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (427 KB) Get e-Alerts Get e-Alerts
Production of Stable Rabbit—Mouse Heterohybridomas: Characterization of a Rabbit Monoclonal Antibody Recognizing a 180 kDa Human Lymphocyte Membrane Antigen (1993)

Polyclonal rabbit antihuman thymocyte globulin (RATG) remains a key component of immunosuppressive strategies in transplantation. The human thymus immunization regimen that produces highly immunosuppressive RATG induces unique antibody specificities in the rabbit. Rabbit monoclonal antibodies (RAb MAbs) to human T cell antigens would be of value in the effort to investigate and reproduce the multiple specificities of RATG. We have fused mouse Sp2/0 cells with splenocytes from rabbits immunized with human thymus and have identified 52 rabbit-mouse heterohybridomas which secrete RAb MAbs directed against human lymphocyte surface antigens. The technical aspects of hybridoma isolation, stabilization and characterization are presented. Analysis by flow cytometry, preabsorption and immunoprecipitation suggests that RAb MAb 1A8 IgG may recognize LFA-1, one of the principal lymphocyte surface antigens recognized by RATG. The 1A8 antigen is 180 kDa and is expressed by 80-90% human PBL and thymocytes. LFA-1 and the 1A8 antigen exhibit 100% co-expression in two-color FACS analysis using four different murine anti-LFA-1 MAbs. 1A8 markedly inhibits the mitogenic response of lymphocytes to PHA, as do murine anti-LFA-1 MAbs. A combination of rabbit antilymphocyte MAbs may potentially reproduce the multiple specificities found in polyclonal RATG and lead to the production of a superior immunosuppressive clinical agent.
Low-dimensional chaos in event-related brain potentials. (1992)

The quantification of a chaotic system, such as the nervous system, can be made by calculating the correlation dimension (D2) from a sample of the data it generates. The encephalogram was recorded from the vertex during an auditory "odd-ball" paradigm and was signal-averaged to reveal the event-related potentials (ERPs). A new method for continuously estimating D2, the "Point-D2" (PD2), was determined from the same data, and it also was signal-averaged. The PD2 method was found to be more accurate than others currently used for investigating finite data; it also was found to track nonstationarities that arise within the data. A significant (p < .001) PD2-decrease accompanied the ERPs evoked by target stimuli; the PD2 onset-latency and peak did not correlate with any ERPs. The very short latency suggests that the PD2-decrease may be associated with "selective stimulus set," an interpretation that has been related to early cortical ERP components.