gebhart/patrepeval huggingface dataset:
<i>In situ</i> polarized 3He system for the Magnetism Reflectometer at the Spallation Neutron Source (2012)

We report on the in situ polarized (3)He neutron polarization analyzer developed for the time-of-flight Magnetism Reflectometer at the Spallation Neutron Source at Oak Ridge National Laboratory. Using the spin exchange optical pumping method, we achieved a (3)He polarization of 76% ± 1% and maintained it for the entire three-day duration of the test experiment. Based on transmission measurements with unpolarized neutrons, we show that the average analyzing efficiency of the (3)He system is 98% for the neutron wavelength band of 2-5 Å. Using a highly polarized incident neutron beam produced by a supermirror bender polarizer, we obtained a flipping ratio of >100 with a transmission of 25% for polarized neutrons, averaged over the wavelength band of 2-5 Å. After the cell was depolarized for transmission measurements, it was reproducibly polarized and this performance was maintained for three weeks. A high quality polarization analysis experiment was performed on a reference sample of Fe/Cr multilayer with strong spin-flip off-specular scattering. Using a combination of the position sensitive detector, time-of-flight method, and the excellent parameters of the (3)He cell, the polarization analysis of the two-dimensional maps of reflected, refracted, and off-specular scattered intensity above and below the horizon were obtained, simultaneously.
Divergence of function and regulation of class B floral organ identity genes. (1997)

Regulatory mechanisms controlling basic aspects of floral morphogenesis seem to be highly conserved among plant species. The class B organ identity genes, which are required to establish the identity of organs in the second (petals) and third (stamens) floral whorls, are a good example of such conservation. This work compares the function of two similar class B genes in the same genetic background. The DEFICIENS (DEF) gene from Antirrhinum, including its promoter, was transformed into Arabidopsis and compared in function and expression with the Arabidopsis class B genes APETALA3 (AP3) and PISTILLATA (PI). The DEF gene was expressed in the second, third, and fourth whorls, as was PI. Functionally, DEF could replace AP3 in making petals and stamens. The DEF gene's AP3-like function and PI-like expression caused transformation of fourth-whorl carpels to stamens. Like AP3, all aspects of DEF function in Arabidopsis required a functional PI protein. Surprisingly, DEF could not replace the AP3 protein in properly maintaining AP3 transcripts (autoregulation). Our data allow us to revise the current model for class B autoregulation and propose a hypothesis for the evolution of class B gene expression in dicotyledonous plants.
[Immunosuppressive macrophages induced in Mycobacterium avium complex infection induced in mice]. (1992)

Profiles of generation and characteristic of splenic macrophages (M phi s), which suppress the ConA mitogenic response of splenic T cells, induced in mice during the course of Mycobacterium avium complex infections were investigated. In MAC-infected mice, reduction of ConA mitogenesis of spleen cells (SPCs) was seen after week 2 and the reduced state thereafter durated as long as at least for 8 weeks. Splenic M phi s with a potent suppressive activity against SPC mitogenesis were transiently induced around 2 weeks after infection. Splenic M phi s of BALB/c (MAC-susceptible) mice had similar but somewhat weaker suppressor activity, as compared to those of CBA/JN (MAC-resistant) mice. The suppressor activity of BALB/c M phi s durated much longer than did CBA/JN M phi s. MAC-induced splenic M phi s were markedly elevated in chemiluminescence and this coincided with increase in immunosuppressive activity. These M phi s inhibited the generation of IL-2-reactive T cell populations in response to ConA-signals, without showing any inhibitory effect against IL-2 producing ability of target T cells. Because immunosuppressive M phi s having the same characteristics were also induced in BALB/c athymic nude mice, thereby indicating that mature T cells are not prerequisite for induction of the present suppressor M phi s in MAC-infected host mice. Prostaglandins (PGs), nitric oxide radical (NO.) and free long chain fatty acids are thought to play certain role in the expression of suppressor action of MAC-induced M phi s, because of the following reasons.(ABSTRACT TRUNCATED AT 250 WORDS)
Infrared characteristic absorption bands of highly isotactic poly(acrylonitrile) (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInfrared characteristic absorption bands of highly isotactic poly(acrylonitrile)Masatomo Minagawa, Kazuyuki Miyano, Masahiko Takahashi, and Fumio YoshiiCite this: Macromolecules 1988, 21, 8, 2387–2391Publication Date (Print):August 1, 1988Publication History Published online1 May 2002Published inissue 1 August 1988https://pubs.acs.org/doi/10.1021/ma00186a015https://doi.org/10.1021/ma00186a015research-articleACS PublicationsRequest reuse permissionsArticle Views492Altmetric-Citations69LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
<i>In situ</i> measurements of GaN nucleation layer decompostion (2003)

GaN nucleation layer (NL) decomposition was measured using optical reflectance over a wide range of pressure P, temperature T, and H2/NH3 gas mixture. The GaN NLs show measurable decomposition above 800 °C and do not significantly roughen until above 960 °C. The NL decomposition rates increase with increasing P, increasing T, and decreasing NH3 flow. An activation energy EA of 2.68 eV was measured (from 820 to 960 °C) for NL decomposition and an EA of 2.62 eV was measured (from 900 to 1075 °C) for decomposition of thick, high-T bulk GaN films. Depending on P, the pre-exponential factor A0 was four to nine times larger for NL decomposition compared to bulk GaN decomposition. The EA measured for both NL and bulk GaN decomposition in mixed H2 and NH3 flows is similar to the EA for Ga desorption, suggesting that the rate-limiting step for both NL and bulk GaN decomposition is Ga desorption.
Transcriptome-wide Profiling and Posttranscriptional Analysis of Hematopoietic Stem/Progenitor Cell Differentiation toward Myeloid Commitment (2014)

Hematopoietic stem cells possess lifelong self-renewal activity and generate multipotent progenitors that differentiate into lineage-committed and subsequently mature cells. We present a comparative transcriptome analysis of ex vivo isolated mouse multipotent hematopoietic stem/progenitor cells (Lin(neg)SCA-1(+)c-KIT(+)) and myeloid committed precursors (Lin(neg)SCA-1(neg)c-KIT(+)). Our data display dynamic transcriptional networks and identify a stem/progenitor gene expression pattern that is characterized by cell adhesion and immune response components including kallikrein-related proteases. We identify 498 expressed lncRNAs, which are potential regulators of multipotency or lineage commitment. By integrating these transcriptome with our recently reported proteome data, we found evidence for posttranscriptional regulation of processes including metabolism and response to oxidative stress. Finally, our study identifies a high number of genes with transcript isoform regulation upon lineage commitment. This in-depth molecular analysis outlines the enormous complexity of expressed coding and noncoding RNAs and posttranscriptional regulation during the early differentiation steps of hematopoietic stem cells toward the myeloid lineage.
Natural Killer Cell Dysfunction and Its Role in COVID-19 (2020)

When facing an acute viral infection, our immune systems need to function with finite precision to enable the elimination of the pathogen, whilst protecting our bodies from immune-related damage. In many instances however this "perfect balance" is not achieved, factors such as ageing, cancer, autoimmunity and cardiovascular disease all skew the immune response which is then further distorted by viral infection. In SARS-CoV-2, although the vast majority of COVID-19 cases are mild, as of 24 August 2020, over 800,000 people have died, many from the severe inflammatory cytokine release resulting in extreme clinical manifestations such as acute respiratory distress syndrome (ARDS) and hemophagocytic lymphohistiocytosis (HLH). Severe complications are more common in elderly patients and patients with cardiovascular diseases. Natural killer (NK) cells play a critical role in modulating the immune response and in both of these patient groups, NK cell effector functions are blunted. Preliminary studies in COVID-19 patients with severe disease suggests a reduction in NK cell number and function, resulting in decreased clearance of infected and activated cells, and unchecked elevation of tissue-damaging inflammation markers. SARS-CoV-2 infection skews the immune response towards an overwhelmingly inflammatory phenotype. Restoration of NK cell effector functions has the potential to correct the delicate immune balance required to effectively overcome SARS-CoV-2 infection.
Low-Dimensional Chalcogenides as Secondary Cathodic Materials: Some Geometric and Electronic Aspects (1986)

▪ Abstract The phase-field method has recently emerged as a powerful computational approach to modeling and predicting mesoscale morphological and microstructure evolution in materials. It describes a microstructure using a set of conserved and ...Read More
Vale George McDonald (1997)

It is with regret that we record the passing of a long-standing member of the Society, Mr George McDonald of Marshall Mount.A man with a keen interest in local history and heritage, Mr McDonald kept his own private museum in the basement of his home, Marshall Mount House, which he willingly opened to members of the IHS.Proud of his own heritage, Mr McDonald and family recently celebrated the centenary of the ramownership of this historic property.
Renal cell carcinoma-associated immune impairment that may interfere with the response to cytokine therapy. (1999)

This prospective study was carried out to explore cytokine-related immune alterations in 69 renal cell carcinoma patients (RCC) and to look for changes which might potentially serve as a reliable predictors of response to cytokine-based therapy. Interleukin-2 (IL-2), its soluble receptor (sIL-2R) and tumor necrosis factor (TNF-alpha) levels produced in vitro by PHA activated and intact mononuclear cells (PBMC) were determined. Concentrations of IL-2, IL-4, IL-6, sIL-2R, TNF-alpha and CRP were measured in sera. Cytokine level was evaluated by enzyme-linked immunoadsorbent assay (ELISA) and CRP was determined by means of turbidimetric method. All measurements were performed in patients without any prior treatment. PHA activated PBMC of RCC patients were significantly defective in producing IL-2 and TNF-alpha comparing to controls (p < 0.03 and p < 0.001). The difference of sIL-2R was noted in metastatic stage only (p < 0.03). Unstimulated PBMC manifested decrease in IL-2 (p < 0.03) and increased level of TNF-alpha in advanced disease (p < 0.02). This impairment reflected tumor size and differentiation stage. Serum concentrations of IL-2, sIL-2R and TNF-alpha were within normal range. However, in relation to the clinical stage, significantly increased serum IL-2 was noted in combined Stage I and II as compared to controls (p = 0.012). IL-6 and CRP showed markedly elevated levels with a significancy which allowed to distinguish samples from metastatic patients. In conclusion careful comparisons of these data with clinical course of cytokine treated patients will disclose which of those tests may possess predictive power in the individual patients who are likely to respond to cytokine-based treatment.
Analysis of Lipids Using 2,4,6-Trihydroxyacetophenone as a Matrix for MALDI Mass Spectrometry (2007)

Lipids exhibit a broad range of chemical properties that make their analysis quite demanding. Today, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) represents a versatile tool in the field of lipid analysis, also offering the possibility for molecular structural identification using novel MALDI tandem time-of-flight (TOF/TOF) instrumentation. In this study, we evaluated 2,4,6-trihydroxyacetophenone (THAP) for the analysis of various lipid classes including neutral storage lipids (triacylglycerols), polar membrane lipids (glycerophospho- and sphingolipids), and glycosphingolipids. THAP proved to be a versatile matrix for the routine analysis of various lipids from biological samples ("lipidomics"). A sample preparation methodology was established using selective alkali salt doping for subsequent MS/MS experiments. Sodiated and lithiated molecules provided superior structural information on lipids (i.e., acyl group identification); thus, following this approach, both selective peak detection with high sensitivity and more reliable structural information were obtained simultaneously.
ESD protection design for mixed-voltage-tolerant I/O buffers with substrate-triggered technique (2004)

A substrate-triggered technique is proposed to improve ESD protection efficiency of the stacked-NMOS device in a mixed-voltage I/O circuit. The substrate-triggered technique can further lower the trigger voltage of the stacked-NMOS device, to ensure effective ESD protection for the mixed-voltage I/O circuit. The proposed ESD protection circuit, with the substrate-triggered technique, for a 2.5 V/3.3 V tolerant mixed-voltage I/O circuit has been fabricated and verified in a 0.25 /spl mu/m salicided CMOS process. Experimental results have confirmed that the HBM ESD robustness of the mixed-voltage I/O circuit can be increased /spl sim/60% by this substrate-triggered design.
Uniqueness and reference immutability for safe parallelism (2012)

A key challenge for concurrent programming is that side-effects (memory operations) in one thread can affect the behavior of another thread. In this paper, we present a type system to restrict the updates to memory to prevent these unintended side-effects. We provide a novel combination of immutable and unique (isolated) types that ensures safe parallelism (race freedom and deterministic execution). The type system includes support for polymorphism over type qualifiers, and can easily create cycles of immutable objects. Key to the system's flexibility is the ability to recover immutable or externally unique references after violating uniqueness without any explicit alias tracking. Our type system models a prototype extension to C# that is in active use by a Microsoft team. We describe their experiences building large systems with this extension. We prove the soundness of the type system by an embedding into a program logic.
Carboxy-Telechelic Polyolefins by ROMP Using Maleic Acid as a Chain Transfer Agent (2011)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTCarboxy-Telechelic Polyolefins by ROMP Using Maleic Acid as a Chain Transfer AgentLouis M. Pitet and Marc A. Hillmyer*View Author Information Department of Chemistry, University of Minnesota, 207 Pleasant St. SE, Minneapolis, Minnesota 55455-0431, United States*E-mail: [email protected]Cite this: Macromolecules 2011, 44, 7, 2378–2381Publication Date (Web):March 7, 2011Publication History Received30 December 2010Revised2 February 2011Published online7 March 2011Published inissue 12 April 2011https://pubs.acs.org/doi/10.1021/ma102975rhttps://doi.org/10.1021/ma102975rbrief-reportACS PublicationsCopyright © 2011 American Chemical SocietyRequest reuse permissionsArticle Views3829Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Carboxyls,Nuclear magnetic resonance spectroscopy,Polymerization,Polymers,Size exclusion chromatography Get e-Alerts
Host-parasite interactions in the pathogenesis of experimental renal candidiasis. (1983)

To study the pathogenesis of renal candidiasis, viable Candida albicans blastospores were injected directly into the left renal artery of New Zealand white rabbits. The progression of the disease was followed by light and electron microscopy over a 6-day period. Within 5 minutes after injection of the yeasts, the organisms localized within glomerular and peritubular capillaries of the cortex. Localization of yeasts within the capillaries occurred through adherence, demonstrated by the presence of surface fibrils originating from the yeast cells. Two to 10 hours later, inflammatory nodules comprised of polymorphonuclear leukocytes formed within capillary lumina. Many of the entrapped yeasts remained viable and extended through adjacent endothelium and epithelium by the formation of germ tubes which penetrated between or directly through intact host cells. After 24 hours, numerous hyphal forms were observed within tubules of the cortex, and some necrotic host cells were noted at sites of penetration. Abscesses replaced renal parenchyma in focal areas during subsequent time intervals. These studies indicate that attachment of Candida albicans to endothelium within capillaries of the cortex is a key event in the disease process. Also, growth of germ tubes into renal tubules provides a temporal advantage for amplification of Candida organisms.
Cervical intraepithelial neoplasia disease progression is associated with increased vaginal microbiome diversity (2015)

Persistent infection with oncogenic Human Papillomavirus (HPV) is necessary for cervical carcinogenesis. Although evidence suggests that the vaginal microbiome plays a functional role in the persistence or regression of HPV infections, this has yet to be described in women with cervical intra-epithelial neoplasia (CIN). We hypothesised that increasing microbiome diversity is associated with increasing CIN severity. llumina MiSeq sequencing of 16S rRNA gene amplicons was used to characterise the vaginal microbiota of women with low-grade squamous intra-epithelial lesions (LSIL; n = 52), high-grade (HSIL; n = 92), invasive cervical cancer (ICC; n = 5) and healthy controls (n = 20). Hierarchical clustering analysis revealed an increased prevalence of microbiomes characterised by high-diversity and low levels of Lactobacillus spp. (community state type-CST IV) with increasing disease severity, irrespective of HPV status (Normal = 2/20,10%; LSIL = 11/52,21%; HSIL = 25/92,27%; ICC = 2/5,40%). Increasing disease severity was associated with decreasing relative abundance of Lactobacillus spp. The vaginal microbiome in HSIL was characterised by higher levels of Sneathia sanguinegens (P < 0.01), Anaerococcus tetradius (P < 0.05) and Peptostreptococcus anaerobius (P < 0.05) and lower levels of Lactobacillus jensenii (P < 0.01) compared to LSIL. Our results suggest advancing CIN disease severity is associated with increasing vaginal microbiota diversity and may be involved in regulating viral persistence and disease progression.
Identification and Isolation of Small CD44-Negative Mesenchymal Stem/Progenitor Cells From Human Bone Marrow Using Elutriation and Polychromatic Flow Cytometry (2013)

The method of isolation of bone marrow (BM) mesenchymal stem/stromal cells (MSCs) is a limiting factor in their study and therapeutic use. MSCs are typically expanded from BM cells selected on the basis of their adherence to plastic, which results in a heterogeneous population of cells. Prospective identification of the antigenic profile of the MSC population(s) in BM that gives rise to cells with MSC activity in vitro would allow the preparation of very pure populations of MSCs for research or clinical use. To address this issue, we used polychromatic flow cytometry and counterflow centrifugal elutriation to identify a phenotypically distinct population of mesenchymal stem/progenitor cells (MSPCs) within human BM. The MSPC activity resided within a population of rare, small CD45⁻CD73⁺CD90⁺CD105⁺ cells that lack CD44, an antigen that is highly expressed on culture-expanded MSCs. In culture, these MSPCs adhere to plastic, rapidly proliferate, and acquire CD44 expression. They form colony forming units-fibroblast and are able to differentiate into osteoblasts, chondrocytes, and adipocytes under defined in vitro conditions. Their acquired expression of CD44 can be partially downregulated by treatment with recombinant human granulocyte-colony stimulating factor, a response not found in BM-MSCs derived from conventional plastic adherence methods. These observations indicate that MSPCs within human BM are rare, small CD45⁻CD73⁺CD90⁺CD105⁺ cells that lack expression of CD44. These MSPCs give rise to MSCs that have phenotypic and functional properties that are distinct from those of BM-MSCs purified by plastic adherence.
Off-line constant current LEDs driver using the HVLED primary controller (2010)

The article introduces an off-line constant current LEDs driver controller, using the HVLED, an innovative all-primary controller, by STMicroelectronics. Using the proprietary patented technique, the IC is able of providing a constant output current using primary sensing feedback, thus eliminating the need for the optocoupler, secondary voltage reference and current sensor, still maintaining quite accurate current regulation. The power supply is able to drive up to 4 LEDs in the European range, i.e. 185-265VAC, even if the same configuration can be also used in U.S. range and for a different numbers of LEDs, only limited from the maximum IC power capability. The proposed driver can be suitably used in all the applications where the tight regulation tolerance, efficiency performances and low component count are decisive to meet the LED's market requirements.