gebhart/patrepeval huggingface dataset:
Insulin modulation of hepatic synthesis and secretion of apolipoprotein B by rat hepatocytes. (1990)

Insulin inhibition of apolipoprotein B (apoB) secretion by primary cultures of rat hepatocytes was investigated in pulse-chase experiments using [35S]methionine as label. Radioactivity incorporation into apoBH and apoBL, the higher and lower molecular weight forms, was assessed after immunoprecipitation of detergent-solubilized cells and media and separation of the apoB forms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hepatocyte monolayers were incubated for 12-14 h in medium with and without an inhibitory concentration of insulin. Cells were then incubated for 10 min with label, and, after differing periods of chase with unlabeled methionine, cellular medium and media labeled apoB were analyzed; greater than 90% of labeled apoB was present in cells at 10 and 20 min after pulse, and labeled apoB did not appear in the medium until 40 min of chase. Insulin treatment inhibited the incorporation of label into total apoB by 48%, into apoBH by 62%, and into apoBL by 40% relative to other cellular proteins. Insulin treatment favored the more rapid disappearance of labeled cellular apoBH with an intra-cellular retention half-time of 50 min (initial half-life of decay, t1/2 = 25 min) compared with 85 min in control (t1/2 = 60 min). Intracellular retention half-times of labeled apoBL were similar in control and insulin-treated hepatocytes and ranged from 80 to 100 min. After 180 min of chase, 44% of labeled apoBL in control and 32% in insulin-treated hepatocytes remained cell associated. Recovery studies indicated that insulin stimulated the degradation of 45 and 27% of newly synthesized apoBH and apoBL, respectively. When hepatocyte monolayers were continuously labeled with [35S]methionine and then incubated in chase medium with and without insulin, labeled apoBH was secreted rapidly, reaching a plateau by 1 h of chase, whereas labeled apoBL was secreted linearly over 3-5 h of chase. Insulin inhibited the secretion of immunoassayable apoB but not labeled apoB. Results demonstrate that 1) insulin inhibits synthesis of apoB from [35S]methionine, 2) insulin stimulates degradation of freshly translated apoB favoring apoBH over apoBL, and 3) an intracellular pool of apoB, primarily apoBL, exists that is largely unaffected by insulin. Overall, insulin action in primary hepatocyte cultures reduces the secretion of freshly synthesized apoB and favors secretion of preformed apoB enriched in apoBL.
Fibre Bragg grating temperature sensors for harsh nuclear environments (2003)

Fibre Bragg grating sensors are being evaluated by the nuclear industry for structural integrity and temperature monitoring. We review radiation effects on FBG sensors and we discuss in-reactor core irradiation of FBG temperature sensors.
High-T/sub c/ coated conductors-performance of meter-long YBCO/IBAD flexible tapes (1999)

One meter long tapes based on 50-100 /spl mu/m thick by 1 cm wide nickel alloy substrates have been coated in a continuous process with a textured yttria-stabilized zirconia layer by ion beam-assisted deposition, followed by a 1-2 /spl mu/m thick layer of YBCO by pulsed laser deposition. The best result to date is a tape with a critical current (I/sub c/) at 75 K of 96 A over an 87 cm measurement length. The overall critical current density and engineering current density are 1 MA/cm/sup 2/ and 10 kA/cm/sup 2/, respectively. Using a special probe, individual I-V curves were generated for each centimeter of tape length in order to investigate longitudinal uniformity of the transport properties: the highest and lowest I/sub c/ values fall within a range of /spl plusmn/25%.
Zur Seitenversetzung des totalreflektierten Lichtstrahles. (Mit 3 Abbildungen ) (1949)

Abstract Die von Goos und Hänchen beobachtete Seitenverschiebung bei der Totalreflexion eines begrenzten Lichtbündels wird im Anschluß an eine Arbeit von C. Schaefer und R. Pich berechnet. Es wird hierbei eine Näherungslösung der Maxwellschen Gleichungen verwandt, die der Begrenztheit des Bündels Rechnung trägt. Die Verschiebung des Strahles ergibt sich anschaulich aus der Betrachtung des Energieströmungsvektors im dünneren Medium. Das Resultat ist das gleiche – auch bezüglich der Polarisationsabhängigkeit des Effektes, – das K. Artmann auf anderem Wege erhielt. Die beiden Theorien geben in gleicher Weise die experimentellen Ergebnisse qualitativ wieder. Im Experiment wird aber keine Polarisationsabhängigkeit gefunden, während die beiden Theorien sie in quantitativer Übereinstimmung behaupten.
Flexible transparent touch panel mounted on round surface (2012)

A flexible transparent touch panel based on conductive liquid channels was developed by a molding process. Transparent silicone rubber was used for producing the channel structures. The height, width, and length of the flow channel were 0.1, 1.0, and 40 mm, respectively. The pitch of the channels was 10 mm. Fabricated two-lined touch panels were stacked at an angle of 90 degrees on a flexible polyimide film. Fabricated flexible transparent touch panels were mounted on both the flat and round surfaces, and touch force detection was evaluated. When a metal rod touched and pushed the surface of the touch panel, the channel structure under the finger elastically deformed. As a result, the electrical resistance in the liquid channel increased with increasing applied normal force.
The performance of a mixed priority real-time scheduling algorithm (1992)

article Free Access Share on The performance of a mixed priority real-time scheduling algorithm Author: Frank W. Miller View Profile Authors Info & Claims ACM SIGOPS Operating Systems ReviewVolume 26Issue 4Oct. 1992 pp 5–13https://doi.org/10.1145/142854.142857Published:01 October 1992Publication History 4citation319DownloadsMetricsTotal Citations4Total Downloads319Last 12 Months12Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Transcriptional Regulation of the Human Polymeric Ig Receptor Gene: Analysis of Basal Promoter Elements (2002)

Secretory Igs provide the first line of adaptive immune defense against ingested, inhaled, and sexually transmitted pathogens at mucosal surfaces. The polymeric Ig receptor regulates transport of dimeric IgA and pentameric IgM into external secretions. The level of expression of polymeric Ig receptor is controlled to a large extent by transcription of the PIGR gene in mucosal epithelial cells. Here we present a detailed analysis of the promoter of the PIGR gene by transient transfection of luciferase reporter plasmids into cultured cell lines. Comparisons of the human and mouse PIGR promoters in human and mouse intestinal and liver cell lines demonstrated that the human PIGR promoter was 4- to 5-fold more active than the mouse PIGR promoter in all cell types, and that both the human and mouse PIGR promoters were more active in intestinal than in liver cell lines. Targeted deletions of 22-bp segments of the human PIGR promoter revealed that the region from nt -63 to -84 is crucial for basal transcription, and that two upstream regions can act as positive or negative regulators. Point mutations within the region from nt -63 to -84 demonstrated that an E box motif, which binds the basic helix-loop-helix protein upstream stimulatory factor, is required for PIGR promoter activity. Two additional regulatory motifs were identified in the proximal promoter region: a binding site for AP2, and an inverted repeat motif that binds an unidentified protein. These findings suggest that cooperative binding of multiple transcription factors regulates basal activity of the human PIGR promoter.
Immunotherapy of Cytotoxic T Cell–resistant Tumors by T Helper 2 Cells (2003)

Currently most attempts at cancer immunotherapy involve the generation of CD8(+) cytotoxic T lymphocytes (CTLs) against tumor-associated antigens. Many tumors, however, have been immunoselected to evade recognition by CTLs and thus alternative approaches to cancer immunotherapy are urgently needed. Here we demonstrate that CD4(+) T cells that recognize a secreted tumor-specific antigen and exhibit a cytokine secretion profile characteristic of Th2 cells, are capable of clearing established lung and visceral metastases of a CTL-resistant melanoma. Clearance of lung metastases by the Th2 cells was found to be totally dependent on the eosinophil chemokine, eotaxin, and partially dependent on the transcription activator signal transducer and activator of transcription 6 (STAT6), with degranulating eosinophils within the tumors inducing tumor regression. In contrast, tumor-specific CD4(+) Th1 cells, that recruited macrophages into the tumors, had no effect on tumor growth. This work provides the basis for a new approach to adoptive T cell immunotherapy of cancer.
A monolithic 12b system DAC (1983)

A microprocessor-addressable monolithic 12b DAC, which achieves a 0.01% voltage output settling in less than 1μs, will be described. Offset, full-scale, gain, temperature coefficient and linearity are internally PROM-trimmed to provide 12b accuracy.
<i>L<sub>A</sub></i> can express every p.r. function (2013)

The built-in resources of LA– the first-order language of basic arithmetic which we first introduced in Section 5.2 – are minimal: there are no non-logical predicates, and just three primitive functions, successor, addition and multiplication.
METABOLIC AND MORPHOLOGICAL OBSERVATIONS ON THE EFFECT OF SURFACE-ACTIVE AGENTS ON LEUKOCYTES (1967)

Morphological and metabolic observations have been made on the effects of endotoxin, deoxycholate, and digitonin (at less than 50 microg/ml) on polymorphonuclear leukocytes and mononuclear cells. The agents stimulate the respiration and glucose oxidation of these cells in a manner similar to that seen during phagocytosis. Electron microscopy revealed no morphological changes with the first two agents, but dramatic membrane changes were seen in the case of digitonin. Here tubular projections of characteristic size and shape formed on and split off the membrane. All the agents stimulated uptake of inulin, but efforts to demonstrate increased pinocytosis by electron microscopy have not so far succeeded, probably due to limitations in present experimental techniques.
Image decomposition, image restoration, and texture modeling using total variation minimization and the H/sup -1/ norm (2004)

We propose a new model for image restoration and decomposition, based on the total variation minimization of Rudin-Osher-Fatemi (1992), and on some new techniques by Y. Meyer (2002) for oscillatory functions. An initial image f is decomposed into a cartoon part u and a texture or noise part v. The u component is modeled by a function of bounded variation, while the v component by an oscillatory function, with bounded H/sup -1/ norm. After some transformation, the resulting PDE is of fourth order. The proposed model continues the ideas and techniques previously introduced by the authors in L Vese et al., (2002). Image decomposition and denoising numerical results will be shown by the proposed new fourth order nonlinear partial differential equation.
Oligonucleotide gap-fill ligation for mutation detection and sequencing<i>in situ</i> (2015)

In clinical diagnostics a great need exists for targeted in situ multiplex nucleic acid analysis as the mutational status can offer guidance for effective treatment. One well-established method uses padlock probes for mutation detection and multiplex expression analysis directly in cells and tissues. Here, we use oligonucleotide gap-fill ligation to further increase specificity and to capture molecular substrates for in situ sequencing. Short oligonucleotides are joined at both ends of a padlock gap probe by two ligation events and are then locally amplified by target-primed rolling circle amplification (RCA) preserving spatial information. We demonstrate the specific detection of the A3243G mutation of mitochondrial DNA and we successfully characterize a single nucleotide variant in the ACTB mRNA in cells by in situ sequencing of RCA products generated by padlock gap-fill ligation. To demonstrate the clinical applicability of our assay, we show specific detection of a point mutation in the EGFR gene in fresh frozen and formalin-fixed, paraffin-embedded (FFPE) lung cancer samples and confirm the detected mutation by in situ sequencing. This approach presents several advantages over conventional padlock probes allowing simpler assay design for multiplexed mutation detection to screen for the presence of mutations in clinically relevant mutational hotspots directly in situ.
Differential adhesion of amino acids to inorganic surfaces (2005)

A fundamental, yet underexplored, materials system is the interface between biological molecules and inorganic surfaces. In an elemental approach to this problem, we have systematically examined the adhesion of amino acids to a series of inorganic surfaces including metals, insulators, and semiconductors. Significant differential adhesion is observed over the full complement of amino acids, determined largely by amino acid side-chain charge. Extensive mapping of the amino acid adhesion versus materials in multiple solutions is presented, with preliminary mechanisms derived from concentration and pH dependence. These results provide an empirical basis for building peptide to inorganic surface structures, and, using this adhesion data, we design inorganic nanostructures that are shown to selectively bind to prescribed primary peptide sequences.
<i>ansa</i>-Zirconocene Complexes with Modified Benzindenyl Ligands:  Syntheses, Crystal Structure, and Properties as Propene Polymerization Catalysts<sup>1</sup><sup>,</sup><sup>2</sup> (1997)

As modifications of the effective catalyst precursor [dimethylsilanediylbis(benz[e]indenyl)]zirconium dichloride, the ansa-zirconocene complexes Me2Si(cyclopenta[c]phenanthryl)2ZrCl2, Me2Si(cyclopenta[l]phenanthryl)2ZrCl2, Me2Si(2-methylcyclopenta[l]phenanthryl)2ZrCl2, and Me2Si(2-methyltetrahydrobenz[e]indenyl)2ZrCl2 have been synthesized. When activated by methylaluminoxane (MAO), these complexes give highly active catalysts for the polymerization of propene to polymers with high isotacticities and molecular weights.
Low-Complexity Decoding via Reduced Dimension Maximum-Likelihood Search (2009)

In this paper, we consider a low-complexity detection technique referred to as a reduced dimension maximum-likelihood search (RD-MLS). RD-MLS is based on a partitioned search which approximates the maximum-likelihood (ML) estimate of symbols by searching a partitioned symbol vector space rather than that spanned by the whole symbol vector. The inevitable performance loss due to a reduction in the search space is compensated by 1) the use of a list tree search, which is an extension of a single best searching algorithm called sphere decoding, and 2) the recomputation of a set of weak symbols, i.e., those ignored in the reduced dimension search, for each strong symbol candidate found during the list tree search. Through simulations on M-quadrature amplitude modulation (QAM) transmission in frequency nonselective multi-input-multioutput (MIMO) channels, we demonstrate that the RD-MLS algorithm shows near constant complexity over a wide range of bit error rate (BER) (10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> ~ 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-4</sup> ), while limiting performance loss to within 1 dB from ML detection.
Inhibition of protein synthesis in rabbit reticulocyte lysates by double-stranded RNA and oxidized glutathione: indirect mode of action on polypeptide chain initiation. (1975)

In the presence of added double-stranded RNA or oxidized glutathione, protein synthesis in heminsupplemented reticulocyte lysates declines abruptly after 8-12 min of incubation at 30 degrees. The kinetics of amino-acid incorporation are very similar to those seen when lysates incorporation are very similar to those seen when lysates are incubated in the absence of added hemin. The inhibitory effects of double-stranded RNA (dsRNA) and oxidized glutathione (GSSG) are partially overcome by a homogeneous initiation factor, IF-MP, which also stimulates protein synthesis in hemin-deficient lysates. This factor is involved in the binding of Met-tRNAfmet to 40S ribosomal subunits during protein chain initiation. However, neither dsRNA alone nor GSSG alone significantly inhibits formation of [40S subunit-Met-tRNAf] complexes induced in reticulocyte lysates by dsRNA or GSSG involves one or more components present in the lysates but absent from the fractionated in vitro system. Such components may be related to the translational inhibitor that is active in hemin-deficient lysates.
I/O placement for FPGAs with multiple I/O standards (2003)

In this paper, we present the first exact algorithm to solve the constrained I/O placement problem for FPGAs that support multiple I/O standards. We derive a compact integer linear programming formulation for the constrained I/O placement problem. The size of the integer linear program derived is independent of the number of I/O objects to be placed and hence is scalable to very large design instances. For example, for a Xilinx Virtex-E FPGA, the number of integer variables required is never more than 32 and is much smaller for practical design instances. Extensive experimental results using a non-commercial integer linear program solver shows that it only takes seconds to solve the resultant integer linear program in practice.
Psoriasis risk factors: role of lifestyle practices. (2000)

Psoriasis is a complex, multifactorial chronic skin disease. As in other chronic disorders, various lifestyle factors have been associated with its morbidity. We have pointed to the significance of the patient's lifestyle practices as they relate to psoriasis outcome. Several reports in the literature suggest that exogenous and endogenous factors, including emotional stress, alcohol use, smoking, and obesity, may have deleterious effects on the increased morbidity of psoriasis. In this study, we carried out a comprehensive evaluation to assess the effects of stress, alcohol use, smoking, obesity, and exercise on the natural history of psoriasis.
Technical Procedures for the Syntheses of Carotenoids and Related Compounds from 6‐Oxo‐isophorone: Syntheses of (3<i>R</i>,3′<i>R</i>)‐Zeaxanthin. Part II (1990)

Abstract Starting from the readily available, optically active (4 R )‐hydroxy‐2,2,6‐trimethylcyclohexanone ( 2 ), a new technical synthesis of (3 R ,3′ R )‐zeaxanthin is described. According to a completely new C 9 +C 2 +C 4 = C 15 scheme, the ketone 2 was protected, ethynylated with Li‐acetylide, and the C 11 ‐intermediate 6 was acetylated, followed by dehydration. The product 10 was protected, deprotonated, and subsequently reacted with methyl vinyl ketone to provide the C 15 ‐propargylate 13 . Reduction in situ of 13 with Vitride yielded the olefinic C 15 ‐alcohol 11 which was transformed into the known C 15 ‐ Wittig salt 3 . A double Wittig reaction of this salt with the C 10 ‐dialdehyde 4 afforded nature‐identical zeaxanthin ( 1 ).
HSV Amplicon Vectors for Cancer Therapy (2006)

HSV amplicon vectors provide a unique tool in the armamentarium of weapons for treatment of cancer. Their large capacity (up to 150 kb) allows incorporation of multiple and large transgenes, including whole gene loci, as well as components of other viruses to control the fate of transgenes in the host cells. Means have been developed to achieve heritable transmission of transgenes in tumor cells by episomal replication or genomic integration. Therapeutic transgenes incorporated into amplicon vectors have included anti-angiogenic agents, immune enhancing proteins, prodrug activating enzymes, and apoptosis-inducing factors, as well as inhibitory RNAs for tumor-associated messages. Perks of this vector system include the ability to combine amplicon vectors with oncolytic HSV recombinant vectors to extend the therapeutic range and to target non-dividing as well as dividing tumor cells. Tumor vaccination is favored by the high infectivity of dendritic antigen-presenting cells with HSV vectors, and the vectors themselves appear to have intrinsic immune enhancing properties. Promoter manipulation can be used to target therapeutic gene expression to specific tumor cell types and to achieve drug regulated transgene expression. Further, amplicon vectors can be used to convert tumor cells into packaging cells for retrovirus and adeno-associated virus vectors, thus generating vectors on site. Amplicon vectors have also proven to be a versatile tool to explore imaging modalities to monitor gene delivery and tumor responses to therapeutic intervention.
Metabolic and energetic benefits of microRNA-22 inhibition (2020)

These original findings suggest that microRNA-22-3p inhibition could lead to a potent treatment of fat accumulation, insulin resistance, and related complex metabolic disorders such as obesity, type 2 diabetes mellitus and non-alcoholic fatty liver disease.
A simple soft-detector for the BLAST system (2002)

The BLAST (Bell-Labs' layered space-time) system is a multi-input multi-output (MIMO) system that can be used to increase the wireless transmission data rate without increasing the total transmission power or bandwidth. In practice, forward error correction (FEC) codes, such as the convolutional code, are often used to lower the bit-error-rate (BER) drastically. We propose a simple soft-detector for QAM constellations based on the unstructured least-squares approach to obtain the soft-information for the Viterbi convolutional decoder. This soft-detector is very simple since it decouples a multi-dimensional QAM symbol detection into multiple one-dimensional QAM symbol detections. Simulation results show that the performance of our soft-decision algorithm can outperform that of the hard-decision sphere decoding algorithm. Yet our algorithm is computationally much more efficient than the latter.
Functionalized Star‐Like Polystyrenes as Organic Supports of a Tridentate Bis(imino)pyridinyliron/Aluminic Derivative Catalytic System for Ethylene Polymerization (2005)

Abstract Summary: Star‐like polystyrenes composed of a microgel core with arms functionalized with a few hydroxy‐ or methoxy‐ended ethylene oxide units were used as organic supports for a tridentate bis(imino)pyridinyliron catalyst towards ethylene polymerization. When used as supports of 2,6‐bis[1‐2,6(diisopropylphenyl)imino]ethylpyridynyl iron dichloride in the presence of various alkylaluminium compounds, the supported catalysts enabled the production, with a high catalytic activity, of polyethylene beads of a spherical morphology and high bulk density. A good control of the polyethylene molar mass distribution could also be achieved, which was explained by a lowering of the transfer reaction to the aluminium derivative, as compared to homogeneous conditions. SEM image of PE particles prepared in the presence of trimethylaluminium supported on a PS microgel with an iron catalyst (TMA/Fe = 800). image SEM image of PE particles prepared in the presence of trimethylaluminium supported on a PS microgel with an iron catalyst (TMA/Fe = 800).