gebhart/patrepeval huggingface dataset:
Non-Covalent Complexes between DNA-Binding Drugs and Double-Stranded Oligodeoxynucleotides:  A Study by ESI Ion-Trap Mass Spectrometry (1999)

We developed an assay that utilizes electrospray ionization mass spectrometry (ESI-MS) to determine rapidly the noncovalent binding of drugs with oligodeoxynucleotides and to assess their relative affinities and stoichiometries. The method uses a set of self-complementary oligodeoxynucleotides that differ in length (6-mer to 12-mer), motif (GC-rich or AT-rich), and sequence, and these were annealed to form duplexes. To the oligodeoxynucleotides are bound a group of drugs (distamycin, Hoechst 33258, Hoechst 33342, berenil and actinomycin D abbreviated as D1, D2, D3, D4, and D5, respectively) that are classic minor-groove binders and intercalators. A second group (porphyrin H2TMpyP-4, metalloporphyrin CuTMpyP-4, FeTMpyP-4 and MnTMpyP-4 and [Ru(II)12S4dppz]Cl2 abbreviated as D6, D7, D8, D9, and D10) binds via mixed modes (i.e., groove binders and intercalators). The results confirm the binding stoichiometry and show preferred binding of minor-groove binders (distamycin, Hoechst 33258, Hoechst 33342, and berenil) to AT-rich oligomers and preferred interaction of the intercalator actinomycin D with GC-rich oligomers. The drugs H2TMpyP-4 and CuTMpyP-4 bind via mixed modes, whereas FeTMpyP-4 and MnTMpyP-4 interact by minor groove-binding only. Competitive binding experiments show that group-I drugs with duplex 5‘-CGCAAATTTGCG-3‘ have binding affinities in the order D3 > D2 > D1 > D4. The order for group-II drugs with duplex 5‘-ATATAT3-‘ is D6 ≈ D7 > D8 ≈ D9.
Real-time video frame rate adaptation based on warping of edge-preserving meshes (2003)

We propose a technique for the real-time synthesis of temporally interpolated video frames suitable for low bandwidth applications. The system is based on the warping of edge preserving meshes. Node selection segments the scene and places mesh nodes on top of object edges in the reference frame. Constrained Delaunay triangulation is used to create a mesh that keeps triangulation edges aligned with object edges in the scene. Node displacements are estimated and several interpolated frames may be synthesized between each pair of original frames without the need for fitting a new mesh to the reference frame. Each of the interpolated frames is synthesized by warping the mesh. We present experimental results that demonstrate the performance and applicability of the proposed method.
SystemC Space Exploration of Behavioral Synthesis Options on Area, Performance and Power consumption (2006)

SystemC based design of system on chip is gaining popularity and designers are increasingly faced with the need to synthesize at the highest level of abstraction. Although RTL level SystemC design synthesis is common behavioral level SystemC design synthesis is still not widely accepted. The objective of this paper is to present methodology for behavioral synthesis of SystemC design. It gives the possibility to detect the best synthesis results on area, performance and power consumption estimation through an automatic exploration of synthesis results. This framework allows SystemC level design space exploration of SoC.
Arc fusion splices with improved strength (4.8 GPa)approaching the strength of the fibre (1995)

For the first time it has been shown that a median splice strength (4.8 GPa) closely matching that of the fibre can be achieved using a commercial fusion splicer. This is achieved by carefully controlling the stripping process and the environmental conditions.
In vivo photoacoustic imaging of mouse embryos (2012)

The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vasculature based upon optical absorption by endogenous hemoglobin, is well suited to this application. In this study, photoacoustic images of mouse embryos were obtained ex vivo and in vivo. The images show intricate details of the embryonic vascular system to depths of up to 10 mm, which allowed whole embryos to be imaged in situ. To achieve this, an all-optical photoacoustic scanner and a novel time reversal image reconstruction algorithm, which provide deep tissue imaging capability while maintaining high spatial resolution and contrast were employed. This technology may find application as an imaging tool for preclinical embryo studies in developmental biology as well as more generally in preclinical and clinical medicine for studying pathologies characterized by changes in the vasculature.
Combinatorial Chemistry (2000)

General Introduction (W. Bannwarth). Combinatorial Chemistry in Solution (W. Bannwarth). Solid--Phase Chemistry (W. Bannwarth). Polymer--Supported Reagents: Preparation and Use in Parallel Organic Synthesis (B. Hinzen). Encoding Strategies for Combinatorial Libraries (B. Hinzen). Automation and Devices for Combinatorial Chemistry and Parallel Organic Synthesis (C. Zechel). Computer--Assisted Library Design (A. Dominik). Organic Reactions on Solid Phase -- A Survey of the Literature from 1970 to 1998 (A. Scannell--Lansky & C. Zechel). References. Index.
Cytokines in single layer amnion allografts compared to multilayer amnion/chorion allografts for wound healing (2014)

Human amniotic membrane allografts have proven effective at improving healing of cutaneous wounds. The mechanism of action for these therapeutic effects is poorly understood but is thought to involve the resident growth factors present in near term amniotic tissue. To determine the relative cytokine contribution of the amnion and chorion in amniotic allografts, the content of 18 cytokines involved in wound healing were measured in samples of PURION® Processed dehydrated amnion, chorion, and amnion/chorion membrane (dHACM) grafts by multiplex enzyme-linked immunosorbent assay array. Both amnion and chorion contained similar amounts of each factor when normalized per dry weight; however, when calculated per surface area of tissue applied to a wound, amnion contained on average only 25% as much of each factor as the chorion. Therefore, an allograft containing both amnion and chorion would contain four to five times more cytokine than a single layer amnion allograft alone. Both single layer amnion and multilayer allografts containing amnion and chorion are currently marketed for wound repair. To examine the role of tissue processing technique in cytokine retention, cytokine contents in representative dehydrated single layer wound care products were measured. The results demonstrated that cytokine content varied significantly among the allografts tested, and that PURION® Processed single layer amnion grafts contained more cytokines than other single layer products. These results suggest that PURION® Processed dHACM contains substantially more cytokines than single layer amnion products, and therefore dHACM may be more effective at delivering growth factors to a healing wound than amnion alone.
A new direction for anticoagulants: Inhibiting fibrin assembly with PEGylated fibrin knob mimics (2011)

Current anticoagulants target coagulation factors upstream from fibrin assembly and polymerization (i.e., formation of fibrin clot). While effective, this approach requires constant patient monitoring since pharmacokinetics and pharmacodynamics vary from patient to patient. To address these limitations, we developed an alternative anticoagulant that effectively inhibits fibrin polymerization. Specifically, we investigated PEGylated fibrin knob "A" peptides, evaluating the effect of both polyethylene glycol (PEG) chain length (0, 2, 5, and 10-30 kDa) and knob peptide sequence (GPRPAAC, GPRPFPAC, and GPRPPERC) on inhibiting fibrin polymerization (i.e., clot formation). Thrombin-initiated clotting assays with purified fibrinogen were performed to compare clot formation with each peptide-PEG conjugate. Results indicated a biphasic effect of PEG chain length, whereby, active-PEG conjugates demonstrated increasingly enhanced inhibition of fibrin polymerization from 0 to 5 kDa PEG. However, the anticoagulant activity diminished to control levels for PEG chains above 5 kDa. Ultimately, we observed a 10-fold enhancement of anticoagulant activity with active peptides PEGylated with 5 kDa PEG compared to non-PEGylated knob peptides. The sequence of the active peptide significantly influenced the anticoagulant properties only at the highest 1:100 molar ratio where GPRPFPAC-5 kDa PEG and GPRPPERC-5 kDa PEG demonstrated significantly lower percent clottable protein than GPRPAAC-5 kDa PEG. Moreover, human plasma treated with the active 5 kDa PEG conjugate exhibited delayed prothrombin time to within the therapeutic range specified for oral anticoagulants. Collectively, this study demonstrated the utility of PEGylated fibrin knob peptides as potential anticoagulant therapeutics. Biotechnol. Bioeng. 2011;108: 2424-2433. © 2011 Wiley Periodicals, Inc.
Human DNA polymerase ε is expressed during cell proliferation in a manner characteristic of replicative DNA polymerases (1995)

In order to shed light on the role of mammalian DNA polymerase epsilon we studied the expression of mRNA for the human enzyme during cell proliferation and during the cell cycle. Steady-state levels of mRNA encoding DNA polymerase epsilon were elevated dramatically when quiescent (G0) cells were stimulated to proliferate (G1/S) in a similar manner to those of DNA polymerase alpha. Message levels of DNA polymerase beta were unchanged in similar experiments. The concentration of immunoreactive DNA polymerase epsilon was also much higher in extracts from proliferating tissues than in those from non-proliferating or slowly proliferating tissues. The level of DNA polymerase epsilon mRNA in actively cycling cells synchronized with nocodazole and in cells fractionated by counterflow centrifugal elutriation showed weaker variation, being at its highest at the G1/S stage boundary. The results presented strongly suggest that mammalian DNA polymerase epsilon is involved in the replication of chromosomal DNA and/or in a repair process that may be substantially activated during the replication of chromosomal DNA. A hypothetical role for DNA polymerase epsilon in a repair process coupled to replication is discussed.
<i>PGR</i>+331 A/G and Increased Risk of Epithelial Ovarian Cancer (2006)

Childbearing and use of oral contraceptives are known to lower the risk of ovarian cancer, and it has been suggested that progesterone or progestin exposures play a role in these associations. The effects of progesterone may be mediated in part through the progesterone receptor, which exists in two functionally distinct protein isoforms, hPR-A and hPR-B. It is known that individuals carrying the A allele of the progesterone receptor gene (PGR) polymorphism, +331 A/G (rs10895068), have greater production of the hPR-B receptor isoform. We therefore examined the association between PGR +331 A/G genotype and risk of ovarian cancer in a population-based study of 490 cases and 534 controls in the state of Connecticut. Adjusted for various reproductive and other factors, a statistically significant increased risk was seen for carriage of the A allele compared with GG genotype [odds ratio (OR), 1.68; 95% confidence interval (95% CI), 1.09-2.59]. When subjects were considered separately according to menopausal status, no increased risk with the A allele was seen for premenopausal women (OR, 0.96; 95% CI, 0.46-2.02) but significantly increased risk was found for postmenopausal women (OR, 2.31; 95% CI, 1.31-4.06). Similar increased risks particularly among postmenopausal women were seen for all histologic tumor types. These findings have been observed before for breast and endometrial cancer, although not for ovary, but still suggest that an hPR-B mechanism may be involved in ovarian neoplasia.
Downlink Transmission Optimization and Statistical Feedback Strategies in a Multi-User IEEE 802.16m System (2009)

A multi-user MIMO/OFDMA system for the next generation broadband wireless access (BWA) networks is studied, in which the base station (BS) has only knowledge of the statistics of the channel. A combination of MIMO, OFDMA and FDD could be suitable to increase spectral efficiency in a high speed network. We investigate methods with scalable channel feedback and we analyze the trade off between the amount of channel state information (CSI) to the transmitter and the system performance. Simulation results demonstrate that substantial gain is obtained by the proposed schemes which take advantage the statistical information of the highly dynamic channel.
Preparation of Magnetite Nanoparticles in Mesoporous Copolymer Template (2001)

Preparation of size-controllable magnetite (Fe3O4) nanoparticles by alkaline oxidation of ferrous ion adsorbed in sulfonated mesoporous styrene−divinylbenzene copolymer is described. It was observed that the magnetite nanoparticle size increases by ion-charging the sulfonated polymeric template with ferrous aqueous solution at increasing iron concentration. A simple model describing the amount of iron incorporation in the polymeric template is proposed. The magnetite-based composite was investigated by atomic absorption, transmission electron microscopy, Mössbauer spectroscopy, X-ray diffraction, and magnetization data.
Does human sperm nuclear DNA integrity affect embryo quality? (2005)

Abnormalities in the male genome are a clear potential reason for post fertilization failure. Male infertility may arise due to high levels of loosely packaged chromatin and damaged DNA. The achievement of a correct chromatin packaging level is essential for successful fertilization. The chromatin contained in the nuclei of mammalian spermatozoa is an extremely compact and stable structure. The reports on mammalian spermatozoa indicate that available volume is insufficient to contain sperm chromatin packed in nucleosome like structure and thus is organized in a special way. Different unique properties of sperm DNA like high degree of inertness and stability, absence of transcription, replacement of somatic histone by protamine etc have made the study of sperm chromatin more interesting. Increased levels of sperm nuclear DNA damage exist in infertile men with abnormal sperm parameters (i.e. concentration, motility and morphology), and various assay techniques have been developed to evaluate sperm chromatin maturity/DNA integrity. These assays are based on the facts that defects in chromatin structure have been shown to lead to increased DNA instability and sensitivity to denaturing stress. DNA integrity in the sperm is essential for the accurate and successful transmission of genetic information. Importance of sperm DNA has also become more obvious in the context of assisted reproductive techniques. While recent advances in assisted reproductive technologies have made possible and practical for many infertile men to become father, the risk of transmission of genetic mutation to the offspring, however, still remains. Further research is necessary to devise techniques for identification and selection of sperm with undamaged DNA for ICSI or to remove sperm with damaged DNA from the semen sample to improve the pregnancy outcome in ICSI.
Experimental determination of ultrasonic wave velocities in plastics as functions of temperature. IV. Shear velocities in common plastics (1967)

Abstract The temperature dependence of acoustic shear velocities are reported for Poly‐Penco nylon, type G Plexiglass [poly(methyl methacrylate)], TFE Teflon (tetrafluoroethylene), low‐ and high‐density polyethylene, Lexan (polycarbonate), and Delrin Acetal (nylon). Most of the data were obtained at a frequency of 1 Mc./sec. over the temperature range of 25–100°C. In addition, previously unreported longitudinal velocities to ∼125°C. are reported for Lexan and Delrin Acetal.
Anti-inflammatory effects of shikonin in human periodontal ligament cells (2018)

SHI possesses anti-inflammatory effects in LPS-stimulated hPDLCs via phospho-ERK and NF-κB/I-κB signaling pathways; this suggests that SHI may hold potential as an anti-inflammatory agent against periodontitis.
Size‐Controlled Water‐Soluble Ag Nanoparticles (2007)

Abstract Ag nanoparticles of two different sizes (1 and 4 nm) were prepared within an apoferritin cavity by using an Ag + ‐loaded apoferritin as a nanoconfined environment for their construction. The initial amount of Ag + ions injected in the apoferritin cavity dictates the size of the final Ag particles. The protein shell prevents bulk aggregation of the metal particles, which renders them water soluble and extremely stable.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Protection of photoreceptors by intravitreal injection of the Y-27632 Rho-associated protein kinase inhibitor in Royal College of Surgeons rats (2015)

Retinitis pigmentosa (RP) is an inherited retinal disease, which is characteristic by degeneration of the rod and cone photoreceptors. The present study aimed to assess the protective effects on photoreceptors of intravitreal injection of Y‑27632, a specific inhibitor of Rho‑associated protein kinase (ROCK), in a Royal College of Surgeons (RCS) rat model. Different concentrations of Y‑27632 (1‑50 mM) were administered by intravitreal injection into the RCS rats. The effects of Y‑27632 were recorded using electroretinography (ERG), measuring the thicknesses of the retinal outer nuclear layer (ONL) and examination of apoptotic markers using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and western blot analysis. Treatment of the eyes with Y27632 at 10 or 50 mM, led to a 30% increase in a‑ and b‑wave amplitudes in ERG, and an increase in ONL thickness by 10%, compared with the 1 mM Y‑27632‑treated and vehicle (phosphate‑buffered saline; PBS)‑treated groups. In addition, eyes treated with 10 mM Y27632 exhibited a 90% decrease in TUNEL‑positive cells, accompanied by decreased protein expression levels of active caspase 3 and Bax by 50%, and a 90% increase in the ratio of Bcl‑2/Bax, compared with the PBS‑treated groups. These data suggested that Y‑27632 protected retinal function by inhibiting the apoptosis of photoreceptor cells in the RCS rat model. The present study demonstrated for the first time, to the best of our knowledge, to report the use of Y‑27632 for protection against RP in an RCS rat model. Y‑27632 may be a potential candidate for the treatment of human RP.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta (2015)

Abstract Diverse progenitor cell populations, including mesenchymal, hematopoietic, trophoblastic, and possibly more primitive stem cells can be isolated from the amniotic fluid and the placenta. At least some of the amniotic and placental cells share a common origin, namely the inner cell mass of the morula. Indeed, most types of progenitor cells that can be isolated from these two sources share many characteristics. This unit will focus solely on the mesenchymal stem cells, the most abundant progenitor cell population found therein and, unlike some of the other stem cell types, present all through gestation. Protocols for isolation, expansion, freezing, and thawing of these cells are presented. Preference is given to the simplest methods available for any given procedure. © 2015 by John Wiley & Sons, Inc.
Stochastic Simulation of Occupant Presence and Behaviour in Buildings (2007)

While most current building simulation tools consider occupants as predictable robots the true nature of human behaviour is more complex. This article describes a set of stochastic models aimed at capturing this complexity by decoupling occupant presence from occupant behaviour, then considering separately each means of occupant interaction (use of appliances, of windows, of lighting, etc.) with the building and finally modeling each of these appropriately. The model of occupant presence is unique in that it generates time series that have proven themselves to be realistic at both hourly and daily time scales. That of window opening assigns personal levels of tolerance to each occupant who thereafter reacts to indoor stimuli. The appliance model attributes devices to a zone, then reproduces the typical use of these by the occupants present, thereby generating a realistic variety in values of energy consumption and peak loads.
Relationship between Particle Size and Efficiency of Palladium-Polyvinyl Alcohol(Pd-PVA) Catalysts (1943)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Sequences of ribosome binding sites from the large size class of reovirus mRNA (1982)

Ribosome-protected fragments from two of the large-sized reovirus mRNAs were recovered from sparsomycin-blocked 80S initiation complexes. The sequence of each protected oligonucleotide was determined. The ribosome binding site of each message includes the m7G cap and a centrally positioned AUG codon. An adenine residue occurs three nucleotides upstream from the initiator codon, thus conforming to the pattern [AG]NNAUG observed for nearly all eucaryotic initiation sites.
Systems approach to metal-based pharmacology (2015)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Engineering against fatigue (1999)

The mechanics and materials approach to fatigue problems in engineering materials aspects of fatigue threshold stress range for short crack growth fatigue strength assessment of AlSi7Mg castings micromechanical modelling of fatigue in nodular cast iron direct observation of the formation of striations fatigue fracture toughness of steels a model for multiaxial small fatigue crack growth evaluation of fatigue life prediction under ideal circumstances compression fatigue damage in notched CFRP laminates surface roughness and crack initiation in fretting fatigue initiation, growth and branching of cracks in railway track prevention of fatigue by surface engineering atmmospheric influence on fatigue crack propagation.
Concurrency control for cooperating transactions in an object-oriented database (1988)

article Concurrency control for cooperating transactions in an object-oriented database Share on Author: A. H. Skarra Brown Univ., Providence, RI Brown Univ., Providence, RIView Profile Authors Info & Claims ACM SIGPLAN NoticesVolume 24Issue 4April 1989 pp 145–147https://doi.org/10.1145/67387.67427Online:26 September 1988Publication History 22citation215DownloadsMetricsTotal Citations22Total Downloads215Last 12 Months7Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access