gebhart/patrepeval huggingface dataset:
Molecular Cloning of Bovine CD14 gene. (1997)

Genomic DNA encoding bovine CD14 was isolated from a bovine (Holstein) genomic library. Utilizing PCR fragment of mouse CD14 gene as a probe, we screened 9 x 10(5) plaques and obtained 3 clones containing the bovine CD14 gene. DNA sequencing showed that bovine CD14 gene encodes 373 amino acids, and the coding sequence was separated by a 90 nt intron. The identity of the deduced amino acid sequence of bovine CD14 was 61-73% to those of mouse, rabbit and human. Northern blot analysis revealed that CD14 mRNA (1.5 kb) was expressed in the lung. Expression of CD14 mRNA was stimulated about 2-fold in bovine peripheral blood macrophage activated with LPS in vitro.
A Bivalent Role of TIP60 Histone Acetyl Transferase in Human Cancer (2015)

Acetylation is a major modification that is required for gene regulation, genome maintenance and metabolism. A dysfunctional acetylation plays an important role in several diseases, including cancer. A group of enzymes-lysine acetyltransferases are responsible for this modification and act in regulation of transcription as cofactors and by acetylation of histones and other proteins. Tip60, a member of MYST family, is expressed ubiquitously and is the acetyltransferase catalytic subunit of human NuA4 complex. This HAT has a well-characterized involvement in many processes, such as cellular signaling, DNA damage repair, transcriptional and cellular cycle. Aberrant lysine acetyltransferase functions promote or suppress tumorigenesis in different cancers such as colon, breast and prostate tumors. Therefore, Tip60 might be a potential and important therapeutic target in the cancer treatment; new histone acetyl transferase inhibitors were identified and are more selective inhibitors of Tip60.
Acoustic attenuation in three-component gas mixtures—Theory (2001)

Vibrational relaxation accounts for absorption and dispersion of acoustic waves in gases that can be significantly greater than the classical absorption mechanisms related to shear viscosity and heat conduction. This vibrational relaxation results from retarded energy exchange between translational and intramolecular vibrational degrees of freedom. Theoretical calculation of the vibrational relaxation time of gases based on the theory of Landau and Teller [Phys. Z. Sovjetunion 10, 34 (1936); 1, 88 (1932): 2, 46 (1932)] and Schwartz et al. [J. Chem. Phys. 20, 1591 (1952)] has been applied at room temperature to ternary mixtures of polyatomic gases containing nitrogen, water vapor, and methane. Due to vibrational-translational and vibrational-vibrational coupling between all three components in ternary mixtures, multiple relaxation processes produce effective relaxation frequencies affecting the attenuation of sound. The dependence of effective relaxation frequencies and the attenuation on mole fractions of the constituents was investigated. The acoustic attenuation in a mixture that is primarily nitrogen is strongly dependent on the concentrations of methane and water vapor that are present. However, the attenuation in a mixture that is primarily methane is only weakly dependent on the concentrations of nitrogen and water vapor. The theory developed in this paper is applicable to other multicomponent mixtures.
Comparative desensitization of the human 5‐HT<sub>2A</sub> and 5‐HT<sub>2C</sub> receptors expressed in the human neuroblastoma cell line SH‐SY5Y (1998)

1. We have used previously characterized clones of the human neuroblastoma cell line, SH-SY5Y. constitutively expressing either the human 5-HT2A or 5-HT2C receptor to compare their desensitization profiles after exposure to 5-HT. 2. 5-HT stimulated [3H]-inositol phosphate ([3H]-IPx) production at both the 5-HT2C (pEC50=8.03+/-0.15) and 5-HT2A receptors (pEC50=7.15+/-0.08), with maximal responses occurring after exposure to 1 microM and 10 microM 5-HT, respectively. 3. Exposure of cells to maximally effective concentrations of 5-HT caused time- and concentration-dependent desensitization of [3H]-IPx formation. The 5-HT2A response desensitized slower (t1/2 = 110 min) and with lower sensitivity than that of the 5-HT2C receptor (t1/2 = 12.5 min). In each case, desensitization was blocked by co-administration of a specific receptor antagonist. Following exposure to 10 microM 5-HT for 2 h, both receptors exhibited extensive desensitization, with subsequent responses to 5-HT reduced by more than 80%. 4. 5-HT stimulated Ins(1,4,5)P3 production with a potency similar to that for [3H]-IPx production at each receptor. In both cases Ins(1,4,5)P3 levels peaked rapidly then returned to basal level within a short time. This peak consistently occurred earlier for the 5-HT2C receptor (5 s) than for the 5-HT2A receptor (20 s). 5. Prior exposure of SH-SY5Y/5-HT2C cells to 5-HT (1 microM/15 min) caused a significant decrease in the 5-HT-stimulated peak in Ins(1,4,5)P3 levels whereas no such change occurred in SH-SY5Y/5-HT2A cells following exposure to 10 microM 5-HT for 15 min. 6 These results indicate that the human 5-HT2A and 5-HT2C receptors both exhibit desensitization at the level of inositol phosphate formation when expressed in the same cellular environment, with the 5-HT2C receptor being more sensitive to 5-HT-mediated desensitization than the 5-HT2A receptor.
Comparative study of coarse head pose estimation (2003)

For many practical applications, it is sufficient to estimate a coarse head pose to infer gaze direction. Indeed, for any application in which the camera is situated unobtrusively in an overhead corner, the only possible inference is coarse pose because of the limitations of the quality and resolution of the incoming data. However, the vast majority of research in head pose estimation deals with tracking full rigid body motion (6 degrees of freedom) for a limited range of motion (typically /spl plusmn/45 degrees out-of-plane) and relatively high resolution data (usually 64/spl times/64 or more). We review the smaller body of research on coarse pose estimation. This work involves image-based learning, estimation of a wide range of pose, and is capable of real-time performance for low-resolution imagery. We evaluate two coarse pose estimation schemes, based on (1) a probabilistic model approach and (2) a neural network approach. We compare the results of the two techniques for varying resolution, head localization accuracy and required pose accuracy. We conclude with details for the implementation specifications for resolution and localization accuracy depending on system accuracy requirements.
Pen harvester for powering a pulse rate sensor (2009)

Rapid developments in the area of micro-sensors for various applications such as structural health monitoring, bio-chemical sensors and pressure sensors have increased the demand for portable, low cost, high efficiency energy harvesting devices. In this paper, we describe the scheme for powering a pulse rate sensor with a vibration energy harvester integrated inside a pen commonly carried by humans in the pocket close to the heart. Electromagnetic energy harvesting was selected in order to achieve high power at lower frequencies. The prototype pen harvester was found to generate 3 mW at 5 Hz and 1 mW at 3.5 Hz operating under displacement amplitude of 16 mm (corresponding to an acceleration of approximately 1.14 grms at 5 Hz and 0.56 grms at 3.5 Hz, respectively). A comprehensive mathematical modelling and simulations were performed in order to optimize the performance of the vibration energy harvester. The integrated pen harvester prototype was found to generate continuous power of 0.46–0.66 mW under normal human actions such as jogging and jumping which is enough for a small scale pulse rate sensor.
Nitrogen, sulphur and carbon uptake from some nitrogenous fertilizers using <sup>15</sup>N, <sup>35</sup>S and <sup>13</sup>C as tracers (1957)

1. Pot culture experiments are described comparing the recovery of nitrogen, and sulphur by the difference and ‘labelled atom’ methods and the recovery of carbon by the latter method. Italian ryegrass was used as the test crop with ammonium sulphate, calcium nitrate and urea as the fertilizers. 2. The recovery of fertilizer nitrogen was less using the ‘labelled atom’ technique than the conventional difference method. 3. The recovery of sulphur from ammonium sulphate ‘labelled’ with 35 S was measured. It was considerably less than the nitrogen, but not inconsiderable especially as a basal dressing of ‘unlabelled’ K 2 SO 4 was applied. 4. Using urea ‘labelled’ with 13 C there was no evidence that any of the carbon was in the ryegrass four weeks after application.
A novel route for the flexible preparation of hydrocarbon jet fuels from biomass-based platform chemicals: a case of using furfural and 2,3-butanediol as feedstocks (2018)

Flexible production of hydrocarbon jet fuels with biomass-derived furfural and 2,3-butanediol as feedstocks by a combination of acid-catalyzed dehydration, aldol condensation, and hydrodeoxygenation.
Random sparse adaptation for accurate inference with inaccurate multi-level RRAM arrays (2017)

An array of multi-level resistive memory devices (RRAMs) can speed up the computation of deep learning algorithms. However, when a pre-trained model is programmed to a real RRAM array for inference, its accuracy degrades due to many non-idealities, such as variations, quantization error, and stuck-at faults. A conventional solution involves multiple read-verify-write (R-V-W) for each RRAM cell, costing a long time because of the slow Write speed and cell-by-cell compensation. In this work, we propose a fundamentally new approach to overcome this issue: random sparse adaptation (RSA) after the model is transferred to the RRAM array. By randomly selecting a small portion of model parameters and mapping them to on-chip memory for further training, we demonstrate an efficient and fast method to recover the accuracy: in CNNs for MNIST and CIFAR-10, ~5% of model parameters is sufficient for RSA even under excessive RRAM variations. As the back-propagation in training is only applied to RSA cells and there is no need of any Write operation on RRAM, the proposed RSA achieves 10-100X acceleration compared to R-V-W. Therefore, this hybrid solution with a large, inaccurate RRAM array and a small, accurate on-chip memory array promises both area efficiency and inference accuracy.
Genomic instability in sporadic colorectal cancer quantitated by inter-simple sequence repeat PCR analysis (1997)

Genomic instability plays a major role in cancer by facilitating tumor progression and tumor heterogeneity. Inter-simple sequence repeat (inter-SSR) PCR has been developed to provide a rapid and reproducible technique for quantitation of the major type of genomic instability observed in sporadic tumors, namely, that manifesting itself as amplifications, deletions, translocations, and insertions. Evaluation of 59 sporadic colorectal cancers by inter-SSR PCR has demonstrated a wide range of instability, independent of tumor stage at diagnosis. Comparison of these data and the results of microsatellite PCR analysis reveals an association of high genomic instability with loss of heterozygosity but no association with the replication error phenomenon arising from defects in mismatch repair.
Regimes of coalescence and separation in droplet collision (1997)

An experimental investigation of the binary droplet collision dynamics was conducted, with emphasis on the transition between different collision outcomes. A series of time-resolved photographic images which map all the collision regimes in terms of the collision Weber number and the impact parameter were used to identify the controlling factors for different outcomes. The effects of liquid and gas properties were studied by conducting experiments with both water and hydrocarbon droplets in environments of different gases (air, nitrogen, helium and ethylene) and pressures, the latter ranging from 0.6 to 12 atm. It is shown that, by varying the density of the gas through its pressure and molecular weight, water and hydrocarbon droplets both exhibit five distinct regimes of collision outcomes, namely (I) coalescence after minor deformation, (II) bouncing, (III) coalescence after substantial deformation, (IV) coalescence followed by separation for near head-on collisions, and (V) coalescence followed by separation for off-centre collisions. The present result therefore extends and unifies previous experimental observations, obtained at one atmosphere air, that regimes II and II do not exist for water droplets. Furthermore, it was found that coalescence of the hydrocarbon droplets is promoted in the presence of gaseous hydrocarbons in the environment, suggesting that coalescence is facilitated when the environment contains vapour of the liquid mass. Collision at high-impact inertia was also studied, and the mechanisms for separation of the coalescence are discussed based on time-resolved collision images. A coalescence/separation criterion defining the transition between regimes III and IV for the head-on collisions was derived and found to agree well with the experimental data.
Some quantitative evaluation of first-pass metabolism of salicylamide in rabbit and rat. (1981)

The first-pass metabolism of salicylamide (SAM) was studied in rabbits and rats paying main attention to the amounts of the glucuronide (SAMG) and sulfate (SAMS) formed. The roles of the intestine and liver for SAMG and SAMS formation during the first-pass were also examined quantitatively. Further, an equation was developed to estimate the hepatic first-pass metabolism based on the experiments of intravenous and oral administration and intestinal perfusion experiment. Significant species differences were found in these two animals. The extent of the first-pass metabolism was larger in rabbits (ca95%) than in rats (ca60%). Of the 95% first-pass metabolism in rabbits, about 30% is due to the intestine whereas the remaining 65% is due to the liver. Of the 60% in rats, about 20 and 40% are estimated to be due to the intestinal and hepatic first-pass metabolism, respectively. In rabbits SAMG and SAMS are formed together in nearly equal amounts in the liver and at the ratio 4 : 1 in the intestine. In rats, on the other hand, SAMG is exclusively formed in the intestine and SAMS in the liver. In this context, the equation described here was applied to the data of Gugler et al. (J. Pharmacol. Exp. Ther., 195, 416-423 (1975)) who carried out a study on the first-pass metabolism of SAM in dogs, and consequently, reported that glucuronidation occurs primarily in the liver whereas sulfation occurs primarily in the intestine in dogs.
Atomic Layer Deposition of PbI<sub>2</sub> Thin Films (2019)

Atomic layer deposition (ALD) enables the deposition of numerous materials in thin film form, yet there are no ALD processes for metal iodides. Herein, we demonstrate an ALD process for PbI2, a metal iodide with a two-dimensional (2D) structure that has applications in areas such as photo-detection and photovoltaics. This process uses lead silylamide Pb(btsa)(2) and SnI4 as precursors and works at temperatures below 90 degrees C, on a variety of starting surfaces and substrates such as polymers, metals, metal sulfides, and oxides. The starting surface defines the crystalline texture and morphology of the PbI2 films. Rough substrates yield porous PbI2 films with randomly oriented 2D layers, whereas smooth substrates yield dense films with 2D layers parallel to the substrate surface. Exposure to light increases conductivity of the ALD PbI2 films which enables their use in photodetectors. The films can be converted into a CH3NH3PbI3 halide perovskite, an important solar cell absorber material. For various applications, ALD offers advantages such as ability to uniformly coat large areas and simple means to control film thickness. We anticipate that the chemistry exploited in the PbI2 ALD process is also applicable for ALD of other metal halides.
RNA-Seq workflow: gene-level exploratory analysis and differential expression (2015)

Here we walk through an end-to-end gene-level RNA-Seq differential expression workflow using Bioconductor packages. We will start from the FASTQ files, show how these were aligned to the reference genome, and prepare a count matrix which tallies the number of RNA-seq reads/fragments within each gene for each sample. We will perform exploratory data analysis (EDA) for quality assessment and to explore the relationship between samples, perform differential gene expression analysis, and visually explore the results.
Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review (2017)

Abstract. Electrical impedance spectroscopy (EIS), in which a sinusoidal test voltage or current is applied to the sample under test to measure its impedance over a suitable frequency range, is a powerful technique to investigate the electrical properties of a large variety of materials. In practice, the measured impedance spectra, usually fitted with an equivalent electrical model, represent an electrical fingerprint of the sample providing an insight into its properties and behavior. EIS is used in a broad range of applications as a quick and easily automated technique to characterize solid, liquid, semiliquid, organic as well as inorganic materials. This paper presents an updated review of EIS main implementations and applications.
<i>Escherichia coli</i> heat‐labile enterotoxin B subunit prevents autoimmune arthritis through induction of regulatory CD4+ T cells (2002)

Mucosal administration of EtxB can be used to prevent or treat CIA. Modulation of the anti-CII immune response by EtxB is associated with a reduction in Th1 cell reactivity without a concomitant shift toward Th2. Instead, EtxB mediates its effects through enhancing the activity of a population of CD4+ regulatory T cells.
Relationship Between Body Condition Score and Composition of Ninth to Eleventh Rib Tissue in Holstein Dairy Cows (1991)

F~fty-six cull Holstein dairy cows with various body condition scores were followed to slaughter. prior to slaughter, cows were weighed on a digital scale, hook to hook and hook to pin dimensions were measured, and age was estimated by dentition. Twenty-four hours after slaughter, 9th to 11th rib sections were collected and weighed, and bone-free tis- sue was dissected and weighed. Deboned tissue was freeze-dried, ground, and analyzed for DM, ether extract, CP, and ash.
Chloroquine, hydroxychloroquine and COVID-19 (2020)

The media have featured the antimalarials chloroquine (CQ) and hydroxychloroquine (HCQ) to treat coronavirus (COVID-19). Political leaders have touted their use and recommended availability to the public. These anti-inflammatory agents have substantial human toxicity with a narrow therapeutic window. CQ and HCQ poisoning cause myocardial depression and profound hypotension due to vasodilation. Bradycardia and ventricular escape rhythms arise from impaired myocardial automaticity and conductivity due to sodium and potassium channel blockade. With cardiotoxicity, ECGs may show widened QRS, atrioventricular heart block and QT interval prolongation. CQ may also cause seizures, often refractory to standard treatment. Of concern is pediatric poisoning, where 1-2 pills of CQ or HCQ can cause serious and potentially fatal toxicity in a toddler. The treatment of CQ/HCQ poisoning includes high-dose intravenous diazepam postulated to have positive ionotropic and antidysrhythmic properties that may antagonize the cardiotoxic effects of CQ. Infusions of epinephrine titrated to treat unstable hypotension, as well as potassium for severe hypokalemia may be required. Current scientific evidence does not support treatment or prophylactic use of these agents for COVID-19 disease. Regulatory and public health authorities recognize that CQ/HCQ may offer little clinical benefit and only add risk requiring further investigation before wider public distribution.
Microfluidic-Based Multiplex qRT-PCR Identifies Diagnostic and Prognostic microRNA Signatures in the Sera of Prostate Cancer Patients (2010)

Recent prostate-specific antigen-based screening trials indicate an urgent need for novel and noninvasive biomarker identification strategies to improve the prediction of prostate cancer behavior. Noncoding microRNAs (miRNA) in the serum and plasma have been shown to have potential as noninvasive markers for physiologic and pathologic conditions. To identify serum miRNAs that diagnose and correlate with the prognosis of prostate cancer, we developed a multiplex quantitative reverse transcription PCR method involving the purification of multiplex PCR products followed by uniplex analysis on a microfluidics chip to evaluate 384 human miRNAs. Using Dgcr8 and Dicer knockout (small RNA-deficient) mouse ES cells as the benchmark, we confirmed the validity of our technique and uncovered a considerable lack of accuracy in previously published methods. Profiling 48 sera from healthy men and untreated prostate cancer patients with differing CAPRA scores, we identified miRNA signatures that allow us to diagnose cancer patients and correlate with a prognosis. These serum signatures include oncogenic and tumor-suppressive miRNAs, suggesting functional roles in prostate cancer progression.
School screening for scoliosis: can surface topography replace examination with scoliometer? (2012)

The study did not reveal advantage of ST as a scoliosis screening method in comparison to clinical examination with the use of the scoliometer.
A Simulation of Nonparametric T-method (1988)

Matsuda(1988)
Monte Carlo simulation of a novel water-equivalent electronic portal imaging device using plastic scintillating fibers (2012)

The results of our simulations show that, using plastic scintillating fibers, it is possible to construct a water-equivalent EPID that has a better energy response and a higher detection efficiency than current flat panel based EPIDs.
Typing of Helicobacter pylori with monoclonal antibodies against Lewis antigens in lipopolysaccharide (1996)

Recently, it has been shown that the lipopolysaccharide (LPS) O antigen of Helicobacter pylori contains Lewis x (Lex), Lewis y (Ley), or both Lex and Ley antigens. We applied a serotyping method for H. pylori by an enzyme-linked immunosorbent assay with monoclonal antibodies (MAbs) specific for these antigens and the related fucosylated H type 1 (H1) antigen. The selected MAbs recognized the Lex and/or Ley structures in the LPS of H. pylori. The agreement between the results of biochemical compositional analysis and the serological data validated our serotyping system. A total of 152 strains from different geographic origins (The Netherlands, Canada, Poland, Italy, and People's Republic of China) were examined for typeability based on the presence of Lewis antigens. One hundred twenty-nine (84.9%) strains were typeable, and 12 different serotyping patterns were observed; 80.9% of the strains contained Lex and/or Le(y) antigens, and 18.4% reacted with the MAb against the related H1 antigen either alone or in combination with the Lex and/or Ley antigen. Our results show that the Lex and Ley antigens are frequently encountered in the LPS of H. pylori strains from various geographic origins. This typing method is an easy-to-perform technique, which can be used for strain differentiation in epidemiological studies of H. pylori infections.
Respiratory muscle training with normocapnic hyperpnea improves ventilatory pattern and thoracoabdominal coordination, and reduces oxygen desaturation during endurance exercise testing in COPD patients (2015)

As expected, NH improves inspiratory muscle performance, exercise capacity, and QoL. New results are significant change in ventilatory pattern, which improves oxygen saturation, and an improvement in thoracoabdominal coordination (lower PhA). These two facts could explain the reduced dyspnea during the endurance test. All these results together may play a role in improving exercise capacity after NH training.