gebhart/patrepeval huggingface dataset:
Caveolin-1 Confers Antiinflammatory Effects in Murine Macrophages via the MKK3/p38 MAPK Pathway (2005)

Caveolin-1 has been reported to regulate apoptosis, lipid metabolism, and endocytosis in macrophages. In the present study, we demonstrate that caveolin-1 can act as a potent immunomodulatory molecule. We first observed caveolin-1 expression in murine alveolar macrophages by Western blotting and immunofluorescence microscopy. Loss-of-function experiments using small interfering RNA showed that down regulating caveolin-1 expression in murine alveolar and peritoneal macrophages increased LPS-induced proinflammatory cytokine TNF-alpha and IL-6 production but decreased anti-inflammatory cytokine IL-10 production. Gain-of-function experiments demonstrated that overexpression of caveolin-1 in RAW264.7 cells decreased LPS-induced TNF-alpha and IL-6 production and augmented IL-10 production. p38 mitogen-activated protein kinase (MAPK) phosphorylation was increased by overexpressing caveolin-1 in RAW264.7 cells, whereas c-Jun N-terminal kinase, extracellular signal-regulated kinase MAPK, and Akt phosphorylation were inhibited. The antiinflammatory modulation of LPS-induced cytokine production by caveolin-1 was significantly abrogated by the administration of p38 inhibitor SB203580 in RAW264.7 cells. Peritoneal macrophages isolated from MKK3 null mice did not demonstrate any modulation of LPS-induced cytokine production by caveolin-1. LPS-induced activation of NF-kappaB and AP-1 determined by electrophoretic mobility shift assay were significantly reduced by overexpressing caveolin-1 in RAW264.7 cells. The reductions were attenuated by the administration of p38 inhibitor SB203580. Taken together, our data suggest that caveolin-1 acts as a potent immunomodulatory effector molecule in immune cells and that the regulation of LPS-induced cytokine production by caveolin-1 involves the MKK3/p38 MAPK pathway.
RELATIVE MERITS OF THE U.S. AND ECE HIGH-BEAM MAXIMUM INTENSITIES AND OF TWO- AND FOUR-HEADLAMP SYSTEMS (2000)

The current maximum high-beam intensity per lamp is 75,000 cd in the United States and 140,000 cd in the ECE and Japan. The primary goal of this study was to evaluate the relative merits of these two intensity levels for visibility and safety. The analysis reviewed evidence related to the following nine factors: visibility without opposing headlights, glare from opposing and following vehicles, dimming distance, sensitivity to degradation, priority between the high and low beams and within the high beam, driver eye-fixations, difference between the intensity of the low beam and the high beam, range of high beam intensities in actual traffic, and high-beam usage. Although some relevant data do not yet exist, the available information generally favors raising the U.S. maximum from 75,000 cd to the current ECE/Japanese level of 140,000 cd. It is also recommended that the minimum high-beam intensity be raised in both the U.S. and the ECE/Japanese regulations in order to improve visibility and safety. The second topic of this study, the relative merits of two- and fourheadlamp systems, is briefly discussed in the Appendix.
Sterols. LX. Oxidation Products of Sarsasapogenin. Structure of C<sub>22</sub> Keto Acid (1939)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSterols. LX. Oxidation Products of Sarsasapogenin. Structure of C22 Keto AcidRussell E. Marker and Ewald. RohrmannCite this: J. Am. Chem. Soc. 1939, 61, 5, 1285–1287Publication Date (Print):May 1, 1939Publication History Published online1 May 2002Published inissue 1 May 1939https://pubs.acs.org/doi/10.1021/ja01874a082https://doi.org/10.1021/ja01874a082research-articleACS PublicationsRequest reuse permissionsArticle Views37Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Malignant Vesical Tumors Following Spinal Cord Injury (1987)

We present 11 male patients with spinal cord injury and neurogenic bladder disease in whom malignant vesical tumors developed. The incidence of these tumors in our spinal cord injury unit was 2.3 per cent. We investigated retrospectively the clinical, endoscopic and radiographic diagnoses, and analyzed the treatment. The most common presenting symptoms were recurrent urinary infections, hematuria and stone disease. Two patients presented with abdominal symptomatology (a large abdominopelvic mass and peritonitis). Endoscopic evaluation was nonspecific in 8 patients (72 per cent). Squamous cell carcinoma was present in 9 patients (81 per cent). One patient had pure transitional cell carcinoma, and 1 had mixed squamous and transitional cell cancer. Cytology was of no value in these patients. The presence of local invasion and bulky disease suggests that ultrasonography or computerized tomography of the abdomen and pelvis should be included during followup. Radical cystoprostatectomy with pelvic lymphadenectomy is the recommended therapy for localized disease. The presence of active or recurrent urethral disease in 55 per cent of the patients indicated that urethrectomy also should be performed.
Differential expression and localization of insulin-like growth factors I and II in cutaneous wounds of diabetic and nondiabetic mice. (1997)

Insulin-like growth factor (IGF)-I has profound effects on tissue repair. IGF-II is felt to exert its influence predominately during fetal development. The purpose of this study was to localize and quantify the expression of IGF-I and IGF-II mRNA and protein during early wound healing in diabetic and nondiabetic mice. The hypothesis is that IGF-I and IGF-II are up-regulated in the healing wound, but their expression is inhibited in diabetics. Full-thickness cutaneous wounds were made on genetically diabetic (C57BL/ KsJ-db/db) mice and their nondiabetic littermates. At various times after wounding, one-half of each wound was fixed and paraffin embedded for immunohistochemistry and in situ hybridization. The other half was flash-frozen for quantification of IGF mRNA by competitive reverse transcriptase polymerase chain reaction and protein by radioimmunoassay. IGF-I mRNA rose sharply in nondiabetics at day 3. Expression in diabetic wounds was significantly delayed until 14 days after wounding. Even then, diabetic IGF-I mRNA levels were 50% less than those in the nondiabetics at their peak. Although not usually considered active in adult life, IGF-II mRNA expression was augmented after wounding, peaking at 3 days in nondiabetics. As with IGF-I, diabetic wounds exhibited a delay in IGF-II mRNA expression, with maximal levels at 10 days after wounding. Interestingly, peak concentrations of IGF-II mRNA were four times greater in diabetics versus nondiabetics. Trends in IGF-I protein expression followed the patterns of mRNA expression. IGF-I levels in nondiabetics were initially double those in diabetics and peaked at 5 days. Diabetic wound concentrations of IGF-I did not peak until 21 days after wounding, at which time they rose to nondiabetic levels. IGF-I and IGF-II proteins were localized to the advancing epithelial edge, to the epithelial cells of adjacent hair follicles, and to the granulation tissue of the wounds. IGF-I and IGF-II mRNA expression was noted in the epithelial edge and in the hair follicles adjacent to the wound, paralleling protein expression. Both IGF-I and IGF-II are up-regulated in the healing wound. A delay in IGF-I and -II presence is noted in the diabetic wound. The impairment in tissue repair in diabetic animals is at least partially due to a deficiency in the production of the IGFs.
The Nitric Oxide Synthase Inhibitor NG-Nitro-L-Arginine Methyl Ester Diminishes the Immunomodulatory Effects of Parental Arginine in Rats with Subacute Peritonitis (2016)

The combined treatment of parenteral arginine and the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) have been shown to improve liver function and systemic inflammation in subacute peritonitic rats. Here, we investigated the effects of single and combined parenteral arginine and L-NAME treatments on leukocyte and splenocyte immunity. Male Wistar rats were subjected to cecal punctures and were intravenously given total parenteral nutrition solutions with or without arginine and/or L-NAME supplementations for 7 days. Non-surgical and sham-operated rats with no cecal puncture were given a chow diet and parenteral nutrition, respectively. Parenteral feeding elevated the white blood cell numbers and subacute peritonitis augmented the parenteral nutrition-induced alterations in the loss of body weight gain, splenomegaly, and splenocyte decreases. Parenteral arginine significantly increased the B-leukocyte level, decreased the natural killer T (NKT)-leukocyte and splenocyte levels, alleviated the loss in body weight gain and total and cytotoxic T-splenocyte levels, and attenuated the increases in plasma nitrate/nitrite and interferon-gamma production by T-splenocytes. L-NAME infusion significantly decreased NKT-leukocyte level, tumor-necrosis factor (TNF)-alpha production by T-splenocytes and macrophages, and interferon-gamma production by T-leukocytes, monocytes, and T-splenocytes, as well as increased interleukin-6 production by T-leukocytes and monocytes and nitrate/nitrite production by T-leukocytes. Combined treatment significantly decreased plasma nitrate/nitrite, the NKT-leukocyte level, and TNF-alpha production by T-splenocytes. Parenteral arginine may attenuate immune impairment and L-NAME infusion may augment leukocyte proinflammatory response, eliminate splenocyte proinflammatory and T-helper 1 responses, and diminish arginine-induced immunomodulation in combined treatment in subacute peritonitic rats.
Abstract 4535: Inhibition of cell proliferation by (R,R')-4′-methoxy-1-naphthylfenoterol in breast cancer cell lines (2014)

Abstract (R,R')-4′-methoxy-1-naphthylfenoterol (MNF) is a bitopic ligand that acts as an agonist of the β2-adrenergic receptor and an antagonist of the orphan G-protein-coupled receptor GPR55. Activation of GPR55 is pro-oncogenic through cannabinoid-related signal transduction and MNF treatment inhibits proliferation of different tumor cell lines, representing cancers of the liver, pancreas and brain. Of significance, MNF exerts antitumor activity in a xenograft glioma model in nude mice. In the current study, the anti-proliferative response mediated by MNF has been examined in human breast cancer cell lines. In this study, MCF-7 and MDA-MB-231 cells were screened for the presence of epidermal growth factor receptor (EGFR) and GPR55 proteins by Western blotting. The EGFR in such cancer cells plays a key role in the control of growth and proliferation. When compared to MCF-7 cells, MDA-MB-231 cells contained high levels of EGFR and GPR55 and showed exquisite sensitivity to MNF as evidenced by the significant reduction in constitutive phosphorylation of the extracellular signal-regulated kinase (ERK1/2), with an IC50 of 0.08 nM. [3H]-Thymidine incorporation assay was performed and revealed that MNF elicited a 2-fold higher growth inhibition in MDA-MB-231 cells as compared to MCF-7 cells (50% vs. 24.9% inhibition at 10 µM of MNF; p&lt;0.001). Evidence for the altered expression and/or signaling pathway activation triggered by EGFR and GPR55 in response to MNF will be presented. We conclude that MDA-MB-231 cells represent a suitable model for the study of possible crosstalk between two classes of pro-oncogenic cell surface receptors and the analysis of MNF as novel therapeutic agent for the management of cancer. This work was supported entirely by the Intramural Research Program of the National Institute on Aging, NIH. Citation Format: Rajib K. Paul, Artur Wnorowski, Michel Bernier, Irving W. Wainer. Inhibition of cell proliferation by (R,R')-4′-methoxy-1-naphthylfenoterol in breast cancer cell lines. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 4535. doi:10.1158/1538-7445.AM2014-4535
Plasmid-mediated virulence in Salmonella dublin demonstrated by use of a Tn5-oriT construct (1985)

Salmonella dublin, a serotype which causes invasive disease in cattle and humans, carries a characteristic 80-kilobase plasmid (pSDL2). We were able to cure the plasmid from a strain of S. dublin. The cured strain was avirulent for mice by either the oral or intraperitoneal route of infection. A derivative of Tn5 which contains the transfer origin of the broad-host-range plasmid RK2 (Tn5-oriT) was transposed onto pSDL2, allowing mobilization of the plasmid by an RK2 helper plasmid. Reintroduction of the pSDL2 derivative plasmid into the cured strain restored virulence, demonstrating that the plasmid is necessary for virulence. These studies also demonstrate the usefulness of the Tn5-oriT construct for genetic manipulations.
Active disturbance rejection control: Methodology, practice and analysis (2014)

An overview of the active disturbance rejection control (ADRC) is presented in this talk. Ever since ADRC was proposed by Jingqing Han in 1990s[1] <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sup> , it has become quite an attractive methodology to applied researchers, even though rigorous theoretical justification was lagging behind for quite some time. The fact that ADRC possesses certain uniqueness in concepts, simplicity in engineering implementation, and superior actual performance allowed it to be readily translated into a valuable asset in engineering practice: namely, the ability in dealing with a vast range of uncertainties, improved transient response, easy implementation and energy savings, to name but a few. The range of applications seems broad, covering almost all domains of control engineering, such as motion control, speed control of induction motor and permanent-magnet synchronous motor, control for noncircular turning process, low-velocity compensation of brushless DC servo, robotic system, underactuated mechanical systems, micro-electro-mechanical systems gyroscope, control for superconducting RF cavities, flight control, attitude tracking of rigid spacecraft, web tension regulation, DC-DC power converter, chemical processes, ALSTOM gasifier benchmark problem, the boilerturbine-generator control systems, stabilization of axial flow compressors, the fractional-order system, etc. [10]–[31]. In the U.S.A., the ADRC solution was recently deployed at a Parker Hannifin Extrusion Plant in North America, resulting in over 50% energy saving per line across ten production lines, together with significant improvements in product quality [32]. And Texas Instrument has adopted the ADRC technology in developing and distributing a new line of motion control chips worldwide [33].The National Superconducting Cyclotron Lab in the U.S. has implemented ADRC in several high energy particle accelerators after significant improvements have been obtained in amplitude and phase regulation of electromagnetic fields. The talk aims to provide the audience with an understanding of the principles behind the success of ADRC and to address questions such as: What's unique about the concept of ADRC? What characterizes the ADRC as a viable technology? And what to expect in the future regarding the technological expansion and the continuous generation of ideas?
New vectors for manipulation and selection of functional yeast artificial chromosomes (YACs) containing human DNA inserts (1995)

A set of fragmentation vectors is described which produce a deletion series of smaller yeast artificial chromosomes (YACs) from a larger parent YAC with the insertion of a eukaryotic selectable marker. In addition, new vectors were designed to permit integration of the genes encoding neomycin (neo) or hygromycin B (hyg) resistance into YACs containing inserts of human DNA. All these vectors are compatible with the yeast host strain AB1380, in which most human genomic YAC libraries are maintained. Linearized vector DNA is used to transform yeast cells in which homologous recombination between human DNA in the YAC and the Alu sequence in the fragmentation or integrating vector produces terminal deletions from the acentromeric (URA3) end of the YAC or insertion of the vector into the YAC, respectively. A set of directional deletions of a YAC is useful for genomic mapping, restriction analysis and functional measurements of large chromosomal regions. The neo and hyg eukaryotic markers permit the study of gene function after introduction of deleted YACs into mammalian cells. Transformation of YACs with the fragmentation vectors resulted in fragmentation in 21-46% of the clones examined; transformation with the integrating vector resulted in integration in 46% of the clones examined.
DNA damage and mutation in human cells exposed to nitric oxide in vitro. (1992)

Nitric oxide (NO.) is a physiological messenger formed by several cell types. Reaction with O2 forms oxides that nitrosate amines at pH values near 7. We now report experiments in which NO. was added to intact human cells and to aerobic solutions of DNA, RNA, guanine, or adenine. TK6 human lymphoblastoid cells were mutated 15- to 18-fold above background levels at both the HPRT and TK gene loci. Xanthine and hypoxanthine, from deamination of guanine and adenine, respectively, were formed in all cases. NO. induced dose-responsive DNA strand breakage. Yields of xanthine ranged from nearly equal to about 80-fold higher than those of hypoxanthine. Yields of xanthine and hypoxanthine from nucleic acids were higher than those from free guanine and adenine. This was most pronounced for xanthine; 0.3 nmol/mg was formed from free guanine vs. 550 nmol/mg from calf thymus RNA. Nitric oxide added to TK6 cells produced a 40- to 50-fold increase in hypoxanthine and xanthine in cellular DNA. We believe that these results, plus the expected deaminations of cytosine to uracil and 5-methylcytosine to thymine, account for the mutagenicity of nitric oxide toward bacteria and mammalian cells.
Flight Test Results for Uniquely Tailored Propulsion Airframe Aeroacoustic Chevrons: Shockcell Noise (2006)

Azimuthally varying chevrons (AVC) which have been uniquely tailored to account for the asymmetric propulsion-airframe aeroacoustic interactions have recently shown significant reductions in jet-related community noise at low-speed take-off conditions in scale model tests of coaxial nozzles with high bypass ratio. There were indications that such AVCs may also provide shockcell noise reductions at high cruise speeds. This paper describes the flight test results when one such AVC concept, namely, the T-fan chevrons with enhanced mixing near the pylon, was tested at full-scale on a modern large twin-jet aircraft (777-300ER) with focus on shockcell noise at mid-cruise conditions. Shockcell noise is part of the interior cabin noise at cruise conditions and its reduction is useful from the viewpoint of passenger comfort. Noise reduction at the source, in the exhaust jet, especially, at low frequencies, is beneficial from the perspective of reduced fuselage sidewall acoustic lining. Results are shown in terms of unsteady pressure spectra both on the exterior surface of the fuselage at several axial stations and also microphone arrays placed inside the fuselage aft of the engine. The benefits of T-fan chevrons, with and without conventional chevrons on the core nozzle, are shown for several engine operating conditions at cruise involving supersonic fan stream and subsonic or supersonic core stream. The T-fan AVC alone provides up to 5 dB low-frequency noise reduction on the fuselage exterior skin and up to 2 dB reduction inside the cabin. Addition of core chevrons appears to increase the higher frequency noise. This flight test result with the previous model test observation that the T-fan AVCs have hardly any cruise thrust coefficient loss (< 0.05%) make them viable candidates for reducing interior cabin noise in high bypass ratio engines.
Energy-Balanced Transmission Policies for Wireless Sensor Networks (2010)

Transmission policy, in addition to topology control, routing, and MAC protocols, can play a vital role in extending network lifetime. Existing transmission policies, however, cause an extremely unbalanced energy usage that contributes to early demise of some sensors reducing overall network's lifetime drastically. Considering cocentric rings around the sink, we decompose the transmission distance of traditional multihop scheme into two parts: ring thickness and hop size, analyze the traffic and energy usage distribution among sensors and determine how energy usage varies and critical ring shifts with hop size. Based on above observations, we propose a transmission scheme and determine the optimal ring thickness and hop size by formulating network lifetime as an optimization problem. Numerical results show substantial improvements in terms of network lifetime and energy usage distribution over existing policies. Two other variations of this policy are also presented by redefining the optimization problem considering: 1) concomitant hop size variation by sensors over lifetime along with optimal duty cycles, and 2) a distinct set of hop sizes for sensors in each ring. Both variations bring increasingly uniform energy usage with lower critical energy and further improves lifetime. A heuristic for distributed implementation of each policy is also presented.
Use of LCD Panel for Calibrating Structured-Light-Based Range Sensing System (2008)

Calibration is a crucial step in structured-light-based range sensing device. The step involves the determination of the intrinsic parameters of both the camera and the projector that constitute the device and the extrinsic parameters between the two instruments. The traditional solution requires the use of an external calibration object with an accurately measured pattern printed on it. This paper presents a calibration design that makes use of a liquid-crystal display (LCD) panel as the calibration object. The LCD panel's planarity is of industrial grade and is thus dependable. The pattern shown on the LCD panel is programmable and is thus convenient to produce in high precision. We show that, with the design, the projector-and-camera system parameters can be calibrated with far fewer images with much higher accuracy. Extensive experiments are shown to illustrate the dramatic improvement in performance.