gebhart/patrepeval huggingface dataset:
Cell surface characteristics of an allotransplantable TA3 ascites subline resulting from a process of immunoselection. (1983)

Comparison of the cell surface characteristics of the parental strain-specific TA3-St ascites cell (I) of the strain A mouse and the more allotransplantable TA3-St/ticol ascites cell (II), immunoselected for reduced absorption of anti-H-2a antibody from Cell I, revealed the following. Cell II, like Cell I, possessed no detectable epiglycanin at its surface, as neither cell absorbed more than 0.5% as much of the antiepiglycanin antibody as was absorbed by the epiglycanin-containing allotransplantable TA3-Ha ascites cell. Tritium-labeled glycoproteins, with polyacrylamide slab gel electrophoresis with sodium dodecyl sulfate and with isoelectric focusing of detergent-treated cells, exhibited marked quantitative differences, but qualitative differences were not established. Glycopeptides cleaved from each cell by proteolysis and fractionated by gel filtration gave similar elution profiles, and the column fractions possessed similar carbohydrate and amino acid compositions. Less sialic acid (170 micrograms/10(9) cells) was removed by neuraminidase from Cell II than from Cell I (270 micrograms/10(9) cells), and the compositions (9% N-glycolylneuraminic acid for Cell II and 20% for Cell I) were different. Transmission and scanning electron microscopy showed rough irregular folds and ridges on the surfaces of each cell, but Cell II appeared more pleomorphic and less rounded than did Cell I. High-resolution transmission electron microscopy showed filamentous material at the surface of Cell II, but not at the surface of Cell I.
Biorecognition through Layer-by-Layer Polyelectrolyte Assembly:  In-Situ Hybridization on Living Cells (2006)

Encapsulated cells were formed from the assembly of cationic and anionic alternating layers using a number of polyelectrolyte-based systems. Chitosan, alginate, hyaluronic acid, and oligonucleotides were used as polyelectrolytes to encapsulate individual E. coli cells, which were used as a model. Zeta potential measurements taken for both chitosan/alginate and chitosan/hyaluronic acid systems indicate successful layer-by-layer (LbL) deposition and gave full reversal of the surface change eight times. Layer adsorption was further observed by fluorescence microscopy, and, through a newly developed protocol for sample preparation, transmission electron microscopy micrographs clearly showed the presence of LbL assembly on the outer layer of the cell membrane, in the nanometer range. A second generation of E. coli cells could be grown from encapsulated first generation cells, demonstrating that the cellular activity was not affected by the presence of polyelectrolyte multilayers. Hybridization between attached oligonucleotide sequences and the complementary sequence was demonstrated by both fluorescence spectroscopy and microscopy. Fluorescence energy transfer data recorded after hybrid formation showed that at a molar ratio of 10:20 (donor:acceptor), Q and I were 92.3% and 52.5%, respectively, which suggests that fluorescein fluorescence was quenched by 92.3% and that the fluorescence of rhodamine was enhanced by 52.5%. Oligonucleotide incorporation was stabilized by deposition of four alternating layers, hence offering not only the potential use of the encapsulated cell as a bio-recognition system but also its application in a number of fields such as oligonucleotide delivery, gene therapy, and the use of DNA as an immunocompatible coating.
A multi-agent home automation system dedicated to power management : Anticipation mechanism (2007)

International audience
Evaluation of Spectrum Occupancy in Indoor and Outdoor Scenario in the Context of Cognitive Radio (2007)

Dynamic spectrum access is an integral part of the Cognitive Radio paradigm. However, efficient spectrum sensing techniques are crucial on the way towards systems, which use idle spectrum bands opportunistically and increase the overall spectral efficiency. Current spectrum occupancy was evaluated in few measurement campaigns at different locations mostly located in the USA. In this paper we report about an extensive measurement campaign conducted in Aachen, Germany, comparing indoor-and outdoor measurement results. The highly sensitive measurement system enabled us to measure also man-made or ambient noise. Since an energy detector cannot differentiate such noise from other primary user signals we determine a very high spectrum occupancy in the outdoor scenario in the band from 20 MHz up to 3 GHz. Considerably less occupation was measured in the indoor scenario also because of less ambient noise. Our measurements confirm that the spectrum band 3-6 GHz is rarely occupied. We further provide a case study how the amplitude probability distribution can be used together with detailed regulatory information to infer additional information about the spectral usage. Such information is beneficial in order to optimize the spectrum sensing process and identify candidate bands for further investigation and possible secondary usage.
Design and modeling of a lateral a-Se MSM photoconductor as indirect conversion X-ray imager (2009)

Amorphous selenium (a-Se) has been widely used as a direct conversion X-ray detection material. Vertical structures are employed in most cases, where >200 μm thick a-Se photoconductor layer is inserted between top and bottom electrodes. In this paper, we design a lateral metal-semiconductor-metal (MSM) structure in which a relatively thin layer of a-Se (~ 8 μm) is coated on top of two lateral electrodes. The simulation results indicate that dark current of such a structure stays extremely low level and external quantum efficiency (EQE) reaches over 30% with wavelengths ranging from 320 to 680 nm. We further fabricate the lateral MSM photoconductor by a two-mask photolithography process. The fabricated photoconductor exhibits a dark current below 40 fA under electric fields ranging from 6 V/μm to 9 V/μm, a responsivity up to 0.06 A/W, a measured EQE of 18% towards a short wavelength of 468 nm, and a high photoresponse speed at 500 Hz with a rise time of 250 μs, fall time of 350 μs, and time constant of 250 μs, respectively. Furthermore, an architecture of indirect conversion X-ray imager is proposed with the use of such a lateral MSM structure and a blue-emitting scintillator material atop.
Estimating exhaust manifold pressure in a turbocharged diesel engine (2003)

The problem of estimating the exhaust manifold pressure in a turbocharged diesel engine is considered. The reciprocated engine causes the air-flow in the exhaust manifold to oscillate with a frequency that is a multiple of the engine speed. As a result of the oscillating airflow the pressure in the exhaust manifold will also oscillate. This oscillation makes it difficult and expensive to measure the pressure. Instead an observer for the exhaust manifold pressure is proposed. The observer is model-based, and the performance of the observer is evaluated by simulations.
Exfoliation and dispersion enhancement in polypropylene nanocomposites by in‐situ melt phase ultrasonication (2004)

Abstract A novel process using ultrasonics to enhance the exfoliation and dispersion of clay platelets in polypropylene‐based nanocomposites has been proposed and investigated. The materials studied were isotactic polypropylene of various molecular weights reinforced with organophilic montmorillonite clay (nanoclay) at 4–6 wt% loadings. X‐ray diffraction (XRD) and rheological measurements, on a model system of nanoclay in mineral oil, were first used to determine ultrasonic energy requirements. The effectiveness of the proposed ultrasonic processing technique on polypropylene nanocomposites was evaluated by XRD and transmission electron microscopy (TEM). The effects of added maleic anhydride–grafted polypropylene compatibilizer, polypropylene molecular weight, and pretreatment of the nanoclays on the nanocomposite exfoliation were also investigated. Results indicate that ultrasonic processing of polymer nanocomposites in the melt state is an effective method for improving exfoliation and dispersion of nanoclays. Issues regarding molecular weight degradation, optimization, mechanical properties, and continuous processing are beyond the scope of the present study and are currently being investigated in our laboratory. Polym. Eng. Sci. 44:1773–1782, 2004. © 2004 Society of Plastics Engineers.
Molecular Cloning and Characterization of 12-Oxophytodienoate Reductase, an Enzyme of the Octadecanoid Signaling Pathway fromArabidopsis thaliana (1997)

Using partial amino acid sequence information for 12-oxophytodienoate-10,11-reductase obtained from Corydalis sempervirens we have cloned the homologous enzyme from Arabidopsis thaliana. The open reading frame of the cDNA encodes a polypeptide of 372 amino acids (Mr = 41,165) with significant similarity to the sequence of Old Yellow Enzyme from Saccharomyces carlsbergensis (Saito, K., Thiele, D. J., Davio, M., Lockridge, O., and Massey, V. (1991) J. Biol. Chem. 266, 20720-20724), a flavin (FMN)-protein catalyzing the NADPH-dependent reduction of the olefinic bond of alpha,beta-unsaturated carbonyls. Specifically, all residues required for binding of FMN in Old Yellow Enzyme are conserved in the A. thaliana sequence, as are all residues associated with catalytic activity. The enzyme was functionally expressed from its cDNA in Escherichia coli and thus proven to encode OPDA reductase. Further similarities of OPDA reductase and yeast Old Yellow Enzyme include their binding to and elution by reductant from N-(4-hydroxybenzoyl)aminohexyl-Sepharose, the immunoreactivity of yeast Old Yellow Enzyme with an antiserum raised against plant OPDA reductase and the demonstration that Old Yellow Enzyme is an active OPDA reductase. It is thus conceivable that the physiological role of Old Yellow Enzymes now known from bacteria, yeasts, and higher plants, is in oxylipin metabolism.
The contemporary spectrum of multiple sclerosis misdiagnosis (2016)

Misdiagnosis of MS leads to unnecessary and potentially harmful risks to patients. Misinterpretation and misapplication of MS clinical and radiographic diagnostic criteria are important contemporary contributors to misdiagnosis.
Synthesis of the three isomeric ortho-substituted phenylthienyl benzoic acids (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of the three isomeric ortho-substituted phenylthienyl benzoic acidsAlexander L. JohnsonCite this: J. Org. Chem. 1976, 41, 8, 1320–1324Publication Date (Print):April 1, 1976Publication History Published online1 May 2002Published inissue 1 April 1976https://pubs.acs.org/doi/10.1021/jo00870a005https://doi.org/10.1021/jo00870a005research-articleACS PublicationsRequest reuse permissionsArticle Views454Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The distribution of stereochemical configurations in polystyrene as observed with <sup>13</sup>C NMR (1975)

Abstract 13 C NMR configurational assignments are made for an amorphous polystyrene sample examined at 25.2 MHz and 120°C. The assignments are based strictly on a one‐parameter Bernoullian fit that was in satisfactory agreement with the nine observed methylene relative intensities. The methylene regions of the 13 C NMR spectra of a polystyrene were examined before and after hydrogenation of the side‐chain phenyl substituents. It was concluded that ring current effects have influenced the 13 C methylene chemical shifts substantially and are limited largely to contributions from adjacent phenyl substituents. In addition, aromatic substituent parameters are reported that can be used in conjunction with the Grant and Paul parameters for calculating chemical shifts in aromatic hydrocarbons and polymers. Finally, it is concluded that free‐radical and n ‐butyllithium‐prepared polystyrenes have essentially atactic structures with meso additions favored over racemic additions by approximately 55/45.
Genome Sequencing and Comparative Analysis of Stenotrophomonas acidaminiphila Reveal Evolutionary Insights Into Sulfamethoxazole Resistance (2018)

<i>Stenotrophomonas acidaminiphila</i> is an aerobic, glucose non-fermentative, Gram-negative bacterium that been isolated from various environmental sources, particularly aquatic ecosystems. Although resistance to multiple antimicrobial agents has been reported in <i>S. acidaminiphila</i>, the mechanisms are largely unknown. Here, for the first time, we report the complete genome and antimicrobial resistome analysis of a clinical isolate <i>S. acidaminiphila</i> SUNEO which is resistant to sulfamethoxazole. Comparative analysis among closely related strains identified common and strain-specific genes. In particular, comparison with a sulfamethoxazole-sensitive strain identified a mutation within the sulfonamide-binding site of <i>folP</i> in SUNEO, which may reduce the binding affinity of sulfamethoxazole. Selection pressure analysis indicated <i>folP</i> in SUNEO is under purifying selection, which may be owing to long-term administration of sulfonamide against <i>Stenotrophomonas</i>.
Selective determination of trace arsenic and antimony species in natural waters by gas chromatography with a photoionization detector (1992)

Abstract Traces amounts of arsenic and antimony in water samples were determined by gas chromatography with a photoionization detector after liquidnitrogen cold trapping of their hydrides. The sample solution was treated with sodium hydroborate (NaBH 4 ) under weak‐acid conditions for arsenic(III) and antimony(III) determination, and under strong‐acid conditions for arsenic(III+V) and antimony(III+V) determination. Large amounts of carbon dioxide (CO 2 ) and water vapor obscured determination of arsine and stibine. Better separation from interference could be achieved by removing CO 2 and water vapor in two tubes containing sodium hydroxide pellets and calcium chloride, respectively. The detection limits of this method were 1.8 ng dm −3 for arsenic and 9.4 ng dm −3 for antimony in the case of 100‐cm 3 sample volumes. Therefore, it is suitable for determination of trace arsenic and antimony in natural waters.
3D reservoir characterization of a North Sea oil field using quantitative seismic & CSEM interpretation (2011)

We have performed a quantitative, joint interpretation of 3D seismic and 3D CSEM data from the Troll Western Oil Province. The presented methodology results in 3D distributions of effective porosity and hydrocarbon saturation. The estimated reservoir property distributions correlate with expected production effects, and we show how hydrocarbon volumes can be assessed.
A process- and temperature-tolerant power-on reset circuit with a flexible detection level higher than the bandgap voltage (2008)

A process- and temperature-tolerant power-on reset (POR) circuit is described. The operation principle of the POR circuit is based on a Kuijk cell bandgap reference with one resistor added to enable a designed output voltage higher than the Bandgap voltage of 1.25V. A test circuit with a detection level of 2.0V was fabricated and measured with a temperature variation of 19mV from 25°C to 90°C and a standard deviation of 25mV across one wafer. The circuit occupies 0.064mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> and dissipates 1.2μA at 90°C.
Human Plasma <i>N</i>-Glycoproteome Analysis by Immunoaffinity Subtraction, Hydrazide Chemistry, and Mass Spectrometry (2005)

The enormous complexity, wide dynamic range of relative protein abundances of interest (over 10 orders of magnitude), and tremendous heterogeneity (due to post-translational modifications, such as glycosylation) of the human blood plasma proteome severely challenge the capabilities of existing analytical methodologies. Here, we describe an approach for broad analysis of human plasma N-glycoproteins using a combination of immunoaffinity subtraction and glycoprotein capture to reduce both the protein concentration range and the overall sample complexity. Six high-abundance plasma proteins were simultaneously removed using a pre-packed, immobilized antibody column. N-linked glycoproteins were then captured from the depleted plasma using hydrazide resin and enzymatically digested, and the bound N-linked glycopeptides were released using peptide-N-glycosidase F (PNGase F). Following strong cation exchange (SCX) fractionation, the deglycosylated peptides were analyzed by reversed-phase capillary liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Using stringent criteria, a total of 2053 different N-glycopeptides were confidently identified, covering 303 nonredundant N-glycoproteins. This enrichment strategy significantly improved detection and enabled identification of a number of low-abundance proteins, exemplified by interleukin-1 receptor antagonist protein (approximately 200 pg/mL), cathepsin L (approximately 1 ng/mL), and transforming growth factor beta 1 (approximately 2 ng/mL). A total of 639 N-glycosylation sites were identified, and the overall high accuracy of these glycosylation site assignments as assessed by accurate mass measurement using high-resolution liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (LC-FTICR) is initially demonstrated.
Local semi-supervised regression for single-image super-resolution (2011)

In this paper, we propose a local semi-supervised learning-based algorithm for single-image super-resolution. Different from most of example-based algorithms, the information of test patches is considered during learning local regression functions which map a low-resolution patch to a high-resolution patch. Localization strategy is generally adopted in single-image super-resolution with nearest neighbor-based algorithms. However, the poor generalization of the nearest neighbor estimation decreases the performance of such algorithms. Though the problem can be fixed by local regression algorithms, the sizes of local training sets are always too small to improve the performance of nearest neighbor-based algorithms significantly. To overcome the difficulty, the semi-supervised regression algorithm is used here. Unlike supervised regression, the information about test samples is considered in semi-supervised regression algorithms, which makes the semi-supervised regression more powerful. Noticing that numerous test patches exist, the performance of nearest neighbor-based algorithms can be further improved by employing a semi-supervised regression algorithm. Experiments verify the effectiveness of the proposed algorithm.
Simulation and animation of sensor-driven robots (1994)

Most simulation and animation systems utilized in robotics are concerned with simulation of the robot and its environment without simulation of sensors. These systems have difficulty in handling robots that utilize sensory feedback in their operation. In this paper, a new design of an environment for simulation, animation, and visualization of sensor-driven robots is presented. As sensor technology advances, increasing numbers of robots are equipped with various types of sophisticated sensors. The main goal of creating the visualization environment is to aid the automatic robot programming and off-line programming capabilities of sensor-driven robots. The software system will help the users visualize the motion and reaction of the sensor-driven robot under their control program. Therefore, the efficiency of the software development is increased, the reliability of the software and the operation safety of the robot are ensured, and the cost of new software development is reduced. Conventional computer-graphics-based robot simulation and animation software packages lack of capabilities for robot sensing simulation. This paper describes a system designed to overcome this deficiency.
Bullous pemphigoid and pemphigus vulgaris: correlated behaviour of serum VEGF, sE-selectin and TNF-alpha levels. (1998)

These data further underline the endothelium involvement in these bullous dermatoses and stress the possibility of employing sE-selectin as a non-specific follow-up marker of both BP and PV.
Thermal behavior of polyaniline films and polyaniline–polystyrene blends (2002)

Abstract In this article, a study of the thermal behavior of polyaniline films and polyaniline–polystyrene blends is presented. Transport measurements (electrical conductivity and thermoelectric power) at high temperature and thermogravimetric analysis show that an irreversible degradation is observed near 450 K for films doped with DiOHP and near 500 K for films doped with CSA. In both cases, the thermoelectric power is the most sensitive parameter to electrical degradation during the heating of conducting films. Electrical conductivity measurements during heating–cooling cycles show a diminution of the room temperature conductivity after evaporation of the solvent (water, m ‐cresol). A model of cluster with a variable diameter allows interpreting this phenomenon by assuming the existence of a sensitive frontier to the solvent at the periphery of conducting clusters. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 84: 1848–1855, 2002; DOI 10.1002/app.10468
Recent Trends in Analytical Atomic Absorption Spectroscopy (1969)

Atomic absorption spectroscopy is reviewed especially as to the effect of developments in the past few years on analytical applications. Sources and sampling devices are given special attention. Atomic absorption, flame emission, and atomic fluorescence are compared as to their detection limits and their applicability to analysis. It is shown that the three methods are in many ways complementary rather than competing. The potentialities of resonance spectroscopy are examined and instrument systems utilizing resonance methods are described.
International Internet Regulation: A Multinational Approach, 16 J. Marshall J. Computer & Info. L. 997 (1998) (1998)

A multinational approach should be applied to Internet regulation. Many countries are concerned about the information that their citizens are exposed to over the Internet. Proponents of international regulation of the Internet claim that two problems must be confronted. First, each country has a different standard of tolerance to egregious information making international regulation difficult. Second, the nature of the Internet does not lend itself to the application of conventional methods of jurisdiction over violators. Certain countries are focusing on regulating Internet information. However, these countries use different methods of Internet regulation. As a result of these differences, Internet regulation must be applied on a multinational level. Furthermore, all countries connected to the Internet must form an international committee to implement a regulatory system. This system would allow nations to exercise varying degrees of regulation over the Internet while maintaining sovereignty.