PD49-01 EFFICIENT PROMOTION OF AUTOPHAGY AND ANGIOGENESIS USING MESENCHYMAL STEM CELLS THERAPY ENHANCED BY THE LOW-ENERGY SHOCKWAVES IN THE TREATMENT OF ERECTILE DYSFUNCTION (2018)

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology II1 Apr 2018PD49-01 EFFICIENT PROMOTION OF AUTOPHAGY AND ANGIOGENESIS USING MESENCHYMAL STEM CELLS THERAPY ENHANCED BY THE LOW-ENERGY SHOCKWAVES IN THE TREATMENT OF ERECTILE DYSFUNCTION Woong Jin Bae, Guan Qun Zhu, Sung Min Kang, Hyuk Jin Cho, U Syn Ha, Sung Hoo Hong, Ji Youl Lee, and Sae Woong Kim Woong Jin BaeWoong Jin Bae More articles by this author , Guan Qun ZhuGuan Qun Zhu More articles by this author , Sung Min KangSung Min Kang More articles by this author , Hyuk Jin ChoHyuk Jin Cho More articles by this author , U Syn HaU Syn Ha More articles by this author , Sung Hoo HongSung Hoo Hong More articles by this author , Ji Youl LeeJi Youl Lee More articles by this author , and Sae Woong KimSae Woong Kim More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2018.02.2317AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Mesenchymal stem cells therapy (MSCT) and defocused low-energy shock wave therapy (ESWT) has been shown to ameliorate erectile dysfunction (ED). However, the interactions and effects of action between MSCT and ESWT remain poorly understood. In this study, we investigated the mechanisms of combination therapy with MSCT and ESWT in a rat model of diabetic ED. METHODS Diabetic rats induced by streptozotocin (50mg/kg) were randomly divided into 5 groups: 1) DM group, 2) DM+ESWT group, 3) DM+MSCT group, 4) DM+ESWT+MSCT group and 5) normal control group. MSCT and (or) ESWT were respectively administered to each group according the proposal for 8 weeks. Immediately after recording of intracavernous pressure (ICP), the penis was then harvested for histologic analysis, ELISA and western blotting. RESULTS The ratio of ICP/MAP was significantly higher in the ESWT+MSCT group than in the ESWT and MSCT only treated groups (p<0.05). And the treatment stimulated angiogenesis and vasodilatation in corpus cavernosum (P<0.05). ESWT increased the quantity of MSCs in the corpus cavernosum and also induced MSCs to express more VEGF in vitro and vivo (p<0.05). Meanwhile, VEGF activated the PI3K/AKT/mTOR and NO/cGMP signaling pathway in the corpus cavernosum. The combination approach stimulated autophagy and decreased apoptosis in the corpus cavernosum. NGF and BDNF expressions were higher in ESWT+MSCT group than in DM group (p<0.01). Furthermore, the treatment promoted the MSCs recruitment by inducing penile tissues to express more PECAM and SDF-1. CONCLUSIONS Combination of LI-ESWT and MSCT can get a better result than a single treatment by expressing more VEGF which can take part in autophagy by triggering the PI3K/AKT/mTOR signaling pathway. This cooperative therapy can provide a new research direction in ED treatment for the future. © 2018FiguresReferencesRelatedDetails Volume 199Issue 4SApril 2018Page: e965 Advertisement Copyright & Permissions© 2018MetricsAuthor Information Woong Jin Bae More articles by this author Guan Qun Zhu More articles by this author Sung Min Kang More articles by this author Hyuk Jin Cho More articles by this author U Syn Ha More articles by this author Sung Hoo Hong More articles by this author Ji Youl Lee More articles by this author Sae Woong Kim More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...