gebhart/patrepeval huggingface dataset:
Purification and properties of UDP-gal:N-acetylgalactosaminide mucin: beta 1,3-galactosyltransferase from swine trachea mucosa. (1982)

A /31,3-galactosyltransferase that transfers galactose from UDP-galactose to N-acetylga1actosaminide:mucin to yield galactosyl /31,3-N-acetylgalactosaminide mucin was purified from swine trachea membrane homogenates.The enzyme which was present in the microsome fraction was solubilized and purified to homogeneity by procedures which included affinity chromatography on Sepharose 4B containing covalently bound asialo Cowper's gland mucin.The enzyme showed a high specificity for N-acetylgalactosaminide residues linked to serine and threonine, and the most active glycosyl acceptors were macromolecular mucin glycoproteins con- taining free N-acetylgalactosaminyl residues linked to a polypeptide chain.The purified enzyme did not transfer galactose to the terminal N-acetylgalactosaminyl residues of type A blood group glycoprotein.Only a single band with molecular weight of 84,000 was observed upon gel electrophoresis in the presence of dodecyl sulfate, and a molecular weight of 90,000 was obtained by exclusion chromatography on Sephadex G-100 in the presence of 0.1% Triton X-100.The solubilized enzyme was unstable in the absence of Triton X-100 or Nonidet P-40.The K, for asialo Cowper's gland mucin was 0.35 PM, and the K,,, for UDP-galactose was 20 PM.The presence of sialic acid-linked a2,6 to the terminal N-acetylgalactosaminide residues of Cowper's gland mucin completely inhibited the transfer of galactose.The product formed in a large scale incubation of asialo Cowper's gland mucin with UDP ['4C]galactose and the purified transferase was isolated.A disaccharide, ["C] galactose /31,3-N-acetylgalactosaminitol, containing more than 90% of the incorporated radioactivity was released by treatment with alkaline borohydride and characterized by enzymatic and methylation analysis.
A preliminary study of the mechanical properties of asphalt mixture containing bottom ash (2008)

This paper shows a preliminary study of asphalt mixtures containing bottom ash with a primary focus on the permanent deformation. The objectives of this study are (i) perform literature reviews on the mechanical properties of asphalt mixtures containing bottom ash; (ii) evaluate the effects of using bottom ash as the mineral filler for the asphalt mixture through the flow number and dynamic modulus tests; and (iii) use the test results to evaluate the pavement permanent deformation using the Mechanistic–empirical pavement design guide (MEPDG) analysis. It was found that the asphalt mixture using bottom ash uses higher asphalt content due to the higher absorption. The replacement with bottom ash in the asphalt mixture generates a lower dynamic modulus as compared to the control mixtures. Additionally, results from MEPDG analysis show mixtures with bottom ash have higher rutting potential.
An Adiponectin Receptor Agonist Reduces Type 2 Diabetic Periodontitis (2019)

Periodontitis is twice as prevalent in diabetics as in nondiabetics, and type 2 diabetes (T2D)-associated periodontitis is severe in many cases due to the altered and aberrant functions of bone cells in hyperglycemic conditions. Therefore, developing an effective method to halt the disease process, as well as restore and regenerate lost alveolar bone to reserve the natural teeth in diabetics, is critically important. In the current study, we applied a newly discovered adiponectin receptor agonist AdipoRon (APR) in experimental periodontitis in diabetic animal models and demonstrated the underlying molecular mechanisms. We found that when APR systemically quenched the blood sugar level in diet-induced obesity (DIO) diabetic mice, it reduced osteoclast numbers and alveolar bone loss significantly due to APR's inhibition on osteoclast differentiation shown in our in vitro studies. APR also decreased the production of proinflammatory molecules CC chemokine ligand 2 and interleukin 6 in diseased gingival tissues. On the other hand, APR promoted alveolar bone regeneration through enhancing osteogenic differentiation and decreasing stromal cell-derived factor 1 in the bone marrow that facilitates stem cell migration. Same results were achieved by APR treatment of periodontitis induced in adiponectin (APN) knockout mice, indicating the ability of APR to activate the endogenous APN receptors to exert osteoanabolic effects. In summary, our study supports the notion that APR could be used as an effective multipronged approach to target T2D-associated periodontitis.
Dual use of superscalar datapath for transient-fault detection and recovery (2001)

Diminutive devices and high clock frequency of future microprocessor generations are causing increased concerns for transient soft failures in hardware, necessitating fault detection and recovery mechanisms even in commodity processors. In this paper, we propose a fault-tolerant extension for modern superscalar out-of-order datapath that can be supported by only modest additional hardware. In the proposed extensions, error-detection is achieved by verifying the redundant results of dynamically replicated threads of executions, while the error-recovery scheme employs the instruction-rewind mechanism to restart at a failed instruction. We study the performance impact of augmenting superscalar microarchitectures with this fault tolerance mechanism. An analytical performance model is used in conjunction with a performance simulator. The simulation results of 11 SPEC95 and SPEC2000 benchmarks show that in the absence of faults, error detection causes a 2% to 45% reduction in throughput, which is in line with other proposed detection schemes. In the presence of transient faults, the fast error recovery scheme contributes very little additional slowdown.
Studies on the behaviour of mist jet and its cooling capacity of hot steel surface (1992)

The present study is concerned with the design of mist nozzle to produce air/water mist jet, and to investigate the behaviour of mist jet and its capacity to cool the hot steel surface. The behaviour of the mist jet is studied by collecting water at different axial distances. Radial water flux profile is correlated with a Gaussian type of distribution function under a wide range of experimental conditions. Maximum water flux in the mist jet is correlated with the downstream axial distance and water‐loading ratio. Water‐loading ratio is found to influence water flux more strongly than axial distance. Radius of the mist jet is correlated with the downstream distance. It is found that the mist jet cools a crack‐sensitive steel without Cracking and, at the same time, produces a martensite type of structure.
Review—Multifunctional Materials for Enhanced Li-Ion Batteries Durability: A Brief Review of Practical Options (2017)

Transition metal (TM) ions dissolution from positive electrodes, migration to and deposition on negative electrodes, followed by Mn-catalyzed reactions of solvents and anions, with loss of Li+ ions, is a major degradation (DMDCR) mechanism in Li-ion batteries (LIBs) with spinel positive electrode materials. While the details of the DMDCR mechanism are still under debate, it is clear that HF and other acid species' attack is the main cause in solutions with LiPF6 electrolyte. We first review the work on various mitigation measures for the DMDCR mechanism, now spanning more than two decades. We then discuss recent progress on our understanding of Mn species in electrolyte solutions and the extension of a mitigation measure first proposed by Tarascon and coworkers in 1999, namely chelation of TM cations, to Mn cation trapping, HF scavenging, and alkali metal ions dispensing multi-functional materials. We focus on practicable, drop-in technical solutions, based on placing such materials in the inter-electrode space, with significant benefits for LIBs performance: increased capacity retention during operation at room and above-ambient temperatures as well as robust (both maximally ionically conducting and electronically insulating) solid-electrolyte interfaces, having reduced charge transfer and film resistances at both negative and positive electrodes. We illustrate the multifunctional materials approach with both new and previously published data. We also discuss and offer our evaluation regarding the merits and drawbacks of the various mitigation measures, with an eye for practically relevant technical solutions capable to meet both the performance requirements and cost constraints for commercial LIBs, and end with recommendations for future work.
Tissue Factor–Factor VIIa Signaling (2004)

How does tissue factor (TF), whose principle role is to support clotting factor VIIa (FVIIa) in triggering the coagulation cascade, affect various pathophysiological processes? One of the answers is that TF interaction with FVIIa not only initiates clotting but also induces cell signaling via activation of G-protein-coupled protease activated receptors (PARs). Recent studies using various cell model systems and limited in vivo systems are beginning to define how TF-VIIa-induced signaling regulates cellular behavior. Signaling pathways initiated by both TF-VIIa protease activation of PARs and phosphorylation of the TF-cytoplasmic domain appear to regulate cellular functions. In the present article, we review the emerging data on the mechanism of TF-mediated cell signaling and how it regulates various cellular responses, with particular focus on TF-VIIa protease-dependent signaling.
Slit Diaphragms Contain Tight Junction Proteins (2009)

Slit diaphragms are essential components of the glomerular filtration apparatus, as changes in these junctions are the hallmark of proteinuric diseases. Slit diaphragms, considered specialized adherens junctions, contain both unique membrane proteins (e.g., nephrin, podocin, and Neph1) and typical adherens junction proteins (e.g., P-cadherin, FAT, and catenins). Whether slit diaphragms also contain tight junction proteins is unknown. Here, immunofluorescence, immunogold labeling, and cell fractionation demonstrated that rat slit diaphragms contain the tight junction proteins JAM-A (junctional adhesion molecule A), occludin, and cingulin. We found these proteins in the same protein complexes as nephrin, podocin, CD2AP, ZO-1, and Neph1 by cosedimentation, coimmunoprecipitation, and pull-down assays. PAN nephrosis increased the protein levels of JAM-A, occludin, cingulin, and ZO-1 several-fold in glomeruli and loosened their attachment to the actin cytoskeleton. These data extend current information about the molecular composition of slit diaphragms by demonstrating the presence of tight junction proteins, although slit diaphragms lack the characteristic morphologic features of tight junctions. The contribution of these proteins to the assembly of slit diaphragms and potential signaling cascades requires further investigation.
On Creating Metadata with Authoring Tools. (2002)

The paper addresses issues of knowledge markup with authoring tools in which users construct representations of their knowledge. It attempts to contribute to the problem by suggesting a methodology to author new documents in a way that they contain markup directly. The methodology is illustrated with a case study, - knowledge markup with an AutoPat tool. AutoPat is an application for authoring technical documents, such as patent claims that guides users towards expert ways of thinking. It consists of two stages, - a semantic authoring module for interactive elicitation of technical knowledge about invention and a syntactic authoring module that automatically generates a legal (syntactically complex) claim text. The semantic authoring module is implemented as a user-friendly interface that can be used as a stand-alone markup tool. The markup includes morpho-syntactic information augmented by semantic data, such as concept (semantic class) and predicate-argument (case-role) structure. A particular focus is set on an easy-to-use environment for markup automation.
A Faster Strongly Polynomial Minimum Cost Flow Algorithm (1993)

In this paper, we present a new strongly polynomial time algorithm for the minimum cost flow problem, based on a refinement of the Edmonds-Karp scaling technique. Our algorithm solves the uncapacitated minimum cost flow problem as a sequence of O(n log n) shortest path problems on networks with n nodes and m arcs and runs in O(n log n(m + n log n)) time. Using a standard transformation, this approach yields an O(m log n(m + n log n)) algorithm for the capacitated minimum cost flow problem. This algorithm improves the best previous strongly polynomial time algorithm, due to Z. Galil and E. Tardos, by a factor of n 2 /m. Our algorithm for the capacitated minimum cost flow problem is even more efficient if the number of arcs with finite upper bounds, say m′, is much less than m. In this case, the running time of the algorithm is O((m′ + n) log n(m + n log n)).
Endogenous prostaglandin endoperoxides and prostacyclin modulate the thrombolytic activity of tissue plasminogen activator. Effects of simultaneous inhibition of thromboxane A2 synthase and blockade of thromboxane A2/prostaglandin H2 receptors in a canine model of coronary thrombosis. (1990)

We tested the hypothesis that simultaneous inhibition of TxA2 synthase and blockade of TxA2/PHG2 receptors is more effective in enhancing thrombolysis and preventing reocclusion after discontinuation of tissue plasminogen activator (t-PA) than either intervention alone. Coronary thrombosis was induced in 35 dogs by placing a copper coil into the left anterior descending coronary artery. Coronary flow was measured with a Doppler flow probe. 30 min after thrombus formation, the animals received saline (controls, n = 10); SQ 29548 (0.4 mg/kg bolus + 0.4 mg/kg per h infusion), a TxA2/PGH2 receptor antagonist (n = 8); dazoxiben (5 mg/kg bolus + 5 mg/kg per h infusion), a TxA2 synthase inhibitor (n = 9); or R 68070 (5 mg/kg bolus + 5 mg/kg per h infusion), a drug that blocks TxA2/PGH2 receptors and inhibits TxA2 synthase (n = 8). Then, all dogs received heparin (200 U/kg) and a bolus of t-PA (80 micrograms/kg) followed by a continuous infusion (8 micrograms/kg per min) for up to 90 min or until reperfusion was achieved. The time to thrombolysis did not change significantly in SQ 29548-treated dogs as compared with controls (42 +/- 5 vs. 56 +/- 7 min, respectively, P = NS), but it was significantly shortened by R 68070 and dazoxiben (11 +/- 2 and 25 +/- 6 min, respectively, P less than 0.001 vs. controls and SQ 29548-treated dogs). R 68070 administration resulted in a lysis time significantly shorter than that observed in the dazoxiben-treated group (P less than 0.01). Reocclusion was observed in eight of eight control dogs, five of seven SQ 29548-treated dogs, seven of nine dazoxiben-treated dogs, and zero of eight R 68070-treated animals (P less than 0.001). TxB2 and 6-keto-PGF1 alpha, measured in blood samples obtained from the coronary artery distal to the thrombus, were significantly increased at reperfusion and at reocclusion in control animals and in dogs receiving SQ 29548. R 68070 and dazoxiben prevented the increase in plasma TxB2 levels, whereas 6-keto-PGF1 alpha levels were significantly increased with respect to control and SQ 29548-treated dogs. Thus, simultaneous inhibition of TxA2 synthase and blockade of TxA2/PGH2 receptors is more effective than either intervention alone in this experimental model in enhancing thrombolysis and preventing reocclusion after t-PA administration.
Transcriptional Profiling of Lipopolysaccharide-Induced Acute Lung Injury (2004)

Mortality associated with acute lung injury (ALI) induced by lipopolysaccharide (LPS) remains high in humans, warranting improved treatment and prevention strategies. ALI is characterized by the expression of proinflammatory mediators and extensive neutrophil influx into the lung, followed by severe lung damage. Understanding the pathogenesis of LPS-induced ALI is a prerequisite for designing better therapeutic strategies. In the present study, we used microarrays to gain a global view of the transcriptional responses of the lung to LPS in a mouse model of ALI that mimics ALI in humans. A total of 71 inflammation-associated genes were up-regulated in LPS-treated lungs, including a chemokine, LPS-induced CXC chemokine (LIX), whose role in the induction of ALI is unknown. Most of the inflammatory genes peaked at 2 h post-LPS treatment. Real-time reverse transcription-PCR confirmed the LPS-induced up-regulation of selected genes identified by microarray analysis, including LIX. The up-regulation of LIX, tumor necrosis factor alpha, and macrophage inflammatory protein 2 was confirmed at the protein level by enzyme-linked immunosorbent assays. To determine the role of LIX in the induction of ALI, we used both exogenous LIX and a LIX blocking antibody. Exogenous LIX alone elicited a neutrophil influx in the lungs, and the anti-LIX antibody attenuated the LPS-induced neutrophil accumulation in the lungs. Taken together, the results of our study demonstrate for the first time the temporal expression of inflammatory genes during LPS-induced ALI and suggest that early therapeutic intervention is crucial to attenuate lung damage. Moreover, we identified a role for LIX in the induction of ALI, and therefore LIX may serve as a novel therapeutic target for the minimization of ALI.
Plasticity in programming of effector and memory CD8<sup>+</sup> T‐cell formation (2010)

CD8(+) T cells (also called cytotoxic T lymphocytes) play a major role in protective immunity against many infectious pathogens and can eradicate malignant cells. The path from naive precursor to effector and memory CD8(+) T-cell development begins with interactions between matured antigen-bearing dendritic cells (DCs) and antigen-specific naive T-cell clonal precursors. By integrating differences in antigenic, costimulatory, and inflammatory signals, a developmental program is established that governs many key parameters associated with the ensuing response, including the extent and magnitude of clonal expansion, the functional capacities of the effector cells, and the size of the memory pool that survives after the contraction phase. In this review, we discuss the multitude of signals that drive effector and memory CD8(+) T-cell differentiation and how the differences in the nature of these signals contribute to the diversity of CD8(+) T-cell responses.
A New c<i>ine</i>-Substitution of Alkenyl Sulfones with Aryltitanium Reagents Catalyzed by Rhodium:  Mechanistic Studies and Catalytic Asymmetric Synthesis of Allylarenes (2003)

The reaction of alkenyl sulfones with aryltitanium triisopropoxide (ArTi(OPr-i )3) in the presence of 3 mol % of [Rh(OH)((S)-binap)]2 in THF at 40 degrees C gave high yield of cine-substitution products. The catalytic cycle was established by deuterium-labeling studies, and it was applied to catalytic asymmetric synthesis of allylarenes which proceeds with over 99% enantioselectivity.
Oxidative degradation of glucose adducts to protein. Formation of 3-(N epsilon-lysino)-lactic acid from model compounds and glycated proteins. (1988)

The chemistry of Maillard or browning reactions of glycated proteins is being studied in model systems in vitro in order to characterize potential reaction pathways and products in biological systems. In previous work with the Amadori rearrangement product N alpha-formyl-N epsilon-fructoselysine (fFL), an analog of glycated lysine residues in proteins, we showed that fFL was oxidatively cleaved between C-2 and C-3 of the carbohydrate chain to yield N epsilon-carboxymethyllysine (CML) and D-erythronic acid. We then detected CML in proteins glycated in vitro, as well as in human lens proteins and collagen in vivo (Ahmed, M. U., Thorpe, S. R., and Baynes, J. W. (1986) J. Biol. Chem. 261, 4889-4894). This work provided an explanation for the origin of CML in human urine and evidence for non-browning pathways of the Maillard reaction in vivo. In this report we describe the identification of a second set of products resulting from oxidative cleavage of fFL between C-3 and C-4 of the sugar chain, i.e. 3-(N epsilon-lysino)-lactic acid (LL) and D-glyceric acid. The formation of LL from fFL was increased at slightly acid pH, representing about 30% of the yield of CML at pH 6.4, compared with 4% at pH 7.4 in phosphate buffer. By gas chromatography-mass spectroscopy, LL was detected in proteins glycated in vitro and then identified as a natural product in human lens proteins and urine. Our results indicate that oxidative degradation of Amadori adducts to proteins occurs in vivo, leading to formation and excretion of CML and LL. These non-browning pathways for reaction of Amadori compounds may be physiologically relevant mechanisms for averting potentially damaging consequences of the Maillard reaction.
Clofazimine Biocrystal Accumulation in Macrophages Upregulates Interleukin 1 Receptor Antagonist Production To Induce a Systemic Anti-Inflammatory State (2016)

Clofazimine (CFZ) is a poorly soluble antibiotic and anti-inflammatory drug indicated for the treatment of leprosy. In spite of its therapeutic value, CFZ therapy is accompanied by the formation of drug biocrystals that accumulate within resident tissue macrophages, without obvious toxicological manifestations. Therefore, to specifically elucidate the off-target consequences of drug bioaccumulation in macrophages, we compared the level of inflammasome activation in CFZ-accumulating organs (spleen, liver and lung) in mice after 2 and 8 weeks of CFZ treatment when the drug exists in soluble and insoluble (biocrystalline) forms, respectively. Surprisingly, the results showed a drastic reduction in caspase 1 and interleukin-1β (IL-1β) cleavage in the livers of mice treated with CFZ for 8 weeks (8-week-CFZ-treated mice) compared to 2-week-CFZ-treated and control mice, which was accompanied by a 3-fold increase in hepatic IL-1 receptor antagonist (IL-1RA) production and a 21-fold increase in serum IL-1RA levels. In the lung and spleen, IL-1β cleavage and tumor necrosis factor alpha expression were unaffected by soluble or biocrystal CFZ forms. Functionally, there was a drastic reduction of carrageenan- and lipopolysaccharide-induced inflammation in the footpads and lungs, respectively, of 8-week-CFZ-treated mice. This immunomodulatory activity of CFZ biocrystal accumulation was attributable to the upregulation of IL-1RA, since CFZ accumulation had minimal effect in IL-1RA knockout mice or 2-week-CFZ-treated mice. In conclusion, CFZ accumulation and biocrystal formation in resident tissue macrophages profoundly altered the host's immune system and prompted an IL-1RA-dependent, systemic anti-inflammatory response.
Reversible Synthesis with Minimum logic function (2006)

A first practical logic function reversible synthesis method has been presented. In this method, minimizing a standard logic function for obtained a SOP of optimization in our approach previously. This efficient conversion between SOP and fixed polarity Reed-Muller (FPRM) forms. The results show that the algorithm is efficient in terms of time and space. We also proposed the synthesis algorithm for reversible functions. It uses fixed polarity Reed-Muller decomposition at each stage to synthesize the function as a network of Toffoli gates. Some examples of NCMC benchmarks with a large number of variables were presented to demonstrate the suitability of the algorithm for synthesizing complex functions