gebhart/patrepeval huggingface dataset:
Glioma cell adhesion and migration on human brain sections. (1998)

Within the brain, dissemination of glioma cells follows myelinated fiber tracts and extracellular matrix containing structures such as the basement membranes of blood vessels. These patterns represent the two major routes of invasion frequently observed in clinical disease. Previously, we have characterized the substrates for preferential glioma adhesion and migration on purified ECM protein. In this study sections of human brain from different anatomical regions were used as adhesive substrates and also characterized for the presence and distribution of matrix proteins. Adhesion of marker gene transfected glioma cell suspensions to different regions and anatomical structures of human brain was quantified using a computer assisted image analysis system. Monoclonal antibodies against different adhesion molecules were used to inhibit glioma cell attachment ot specific anatomical structures. In addition, glioma cell aggregates were allowed to adhere to brain sections and single cells were observed to migrate out of these aggregates. Scanning electron microscopy was used to morphologically study the preferred routes of glioma dissemination on brain sections. In brain sections different kinetics of cell adhesion to distinct structures were observed. Within 15 minutes cells adhered and spread on blood vessels and arachnoid tissue containing sections. Choroid plexus and the ventricular wall were also adhesive structures. Adhesion to cortex required 1 hour, while adhesion and spreading on myelinated fiber tracts was retarded and required several hours of incubation. The predominant matrix proteins in small vessels were found to be laminin, collagen type IV, and fibronectin. Choroid plexus and the ependyma showed a similar composition of matrix proteins. Arachnoid fibers contained different types of collagens, predominately type I and III, whereas the only matrix protein identified in the subependyma was fibronectin. Antibodies to the alpha 2, alpha 3, and beta 1 integrin subunits completely blocked adhesion to arachnoid tissue, anti-NCAM inhibited attachment to cortex. Adhesion to blood vessels in brain sections could only be inhibited to 50% by anti-integrin beta 1. Antibodies to the av containing integrin av beta 3 also blocked 50% of adhesion to vessels. Our findings indicate that adhesion of glioma cells to brain sections most rapidly takes place on ECM protein containing regions, especially blood vessels which may serve as guiding structures for glioma dissemination.
Arc of a Vicious Circle (2011)

In this review, we examine how a subset of signal transduction cascades initiated by Mycobacterium tuberculosis (Mtb) infection modulates transcription mediated by the human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR). We describe two distinct phases of signaling that target transcription factors known to bind the HIV-1 LTR, and thus drive viral transcription and replication, in cells of the Mtb-infected host. First, Mtb-derived molecules, including cell wall components and DNA, interact with a number of host pattern recognition receptors. Second, cytokines and chemokines secreted in response to Mtb infection initiate signal transduction cascades through their cognate receptors. Given the variation in cell wall components among distinct clinical Mtb strains, the initial pattern recognition receptor interaction leading to direct LTR activation and differential cytokine and chemokine production is likely to be an important aspect of Mtb strain-specific regulation of HIV-1 transcription and replication. Improved understanding of these molecular mechanisms in the context of bacterial and host genetics should provide key insights into the accelerated viral replication and disease progression characteristic of HIV/TB coinfection.
Ganglioside Increases Metastatic Potential and Susceptibility of Prostate Cancer to Gene Therapy in vitro (2009)

Prostate cancer (CaP) is the 2nd most common cancer in North American men. Tumour management strategies are appropriate for early stage disease, but advanced disease has a poor prognosis and requires prompt treatment. Therefore, research into delay of tumour progression and efficacious treatment of aggressive cancer are of interest. Ganglioside was assessed for its role in altering markers of metastatic potential and susceptibility of CaP to adenovirus-mediated gene therapy. Healthy (RWPE-1) and malignant (DU-145, PC-3) prostate cells were cultured with or without mixed ganglioside. Differences in growth, ganglioside and integrin densities, and adenoviral infectivity were assessed between treatment and control groups. Ganglioside decreased (p<0.01) growth of PC-3 cells relative to untreated control. Ganglioside decreased (p<0.01) GD1a and increased (p<0.04) integrin densities in malignant prostate cells, suggesting ganglioside may increase metastatic potential of CaP. Ganglioside significantly increased adenovirus entry in PC-3 cells, thereby improving susceptibility of CaP to adenovirus-mediated gene therapy.
Effect of deoxycholic acid and ursodeoxycholic acid on lipid peroxidation in cultured macrophages. (1996)

Hydrophobic bile acids, after their hepatic retention, can oxidatively activate Kupffer cells to generate reactive oxygen species. Because UDCA can block this action, the beneficial effect of UDCA is, in part, related to its ability to act as an antioxidant.
Integrating oscillatory integrands of a non-trigonometric type over a finite range (1992)

A method is developed for the numerical integration of non-trigonometric oscillatory integrands with special emphasis on Bessel-type oscillations. The basis of the approach is a Chebeshev fit to the non-oscillatory part of the integrand, and the work is an extension of a previous method for trigonometric weight functions. A stable means of computing the required monomial integrals is presented, together with a representative set of test examples and comparisons.
Cultural Influences and the Built Environment: An Examination of Kumasi, Ghana (2005)

This paper examines indigenous and colonial influences on the development and use of urban space in Kumasi, Ghana as a historical narrative. It is argued that sanitary concern, the creation of a culturally recognizable landscape and residential segregation, through the importation of contemporary English planning practices and ideas, drove residential planning decisions. The paper then proceeds to examine these spaces as they are today, noting that they have either been abandoned or re-appropriated through the reassertion of long-standing cultural traditions. The characteristics of such spaces are next examined, noting that flexibility, multi-purpose, functionality and location all play a role in creating a successful urban space.
Cooperative video streaming on smartphones (2011)

Video applications are increasingly popular over smartphones. However, in current cellular systems, the downlink data rate fluctuates and the loss rate can be quite high. We are interested in the scenario where a group of smartphone users, within proximity of each other, are interested in viewing the same video at the same time and are also willing to cooperate with each other. We propose a system that maximizes the video quality by appropriately using all available resources, namely the cellular connections to the phones as well as the device-to-device links that can be established via Bluetooth or WiFi. Key ingredients of our design are: (i) the cooperation among users, (ii) network coding, and (iii) exploiting broadcast in the mobile-to-mobile links. Our approach is grounded on a network utility maximization formulation of the problem. We present numerical results that demonstrate the benefit of our approach, and we implement a prototype on android phones.
Emerging drugs for the treatment of axial and peripheral spondyloarthritis (2015)

Since, in contrast to rheumatoid arthritis, the therapeutic array in SpA is currently limited to TNF-blockers, and since there is still an unmet need because some patients do not respond to anti-TNF therapy at all or they loose response, new agents with a different mechanism of action are eagerly awaited.
The selectivity filter of the N-methyl-D-aspartate receptor: a tryptophan residue controls block and permeation of Mg2+. (1998)

A hallmark feature of N-methyl-D-aspartate (NMDA) receptors is their voltage-dependent block by extracellular Mg2+. The structural basis for Mg2+ block is not fully understood. Although asparagine residues in the pore-forming M2 regions of NR1 and NR2 subunits influence Mg2+ block, it has been speculated that additional residues are likely to be involved. Here, we report the unexpected finding that a tryptophan residue in the M2 region of NR2 subunits controls Mg2+ block. An NR2B(W607L) mutation abolished block and greatly increased permeation of extracellular Mg2+. A similar effect was seen with a mutation at the equivalent residue in NR2A but not with mutations at the equivalent residue or adjacent residues in NR1. In NR2B, mutations that changed NR2B(W607) to asparagine (W607N) or alanine (W607A) also greatly reduced Mg2+ block, whereas mutations that changed W607 to the aromatic residues tyrosine (W607Y) or phenylalanine (W607F) had little or no effect on Mg2+ block. Furthermore, the W607L, W607N, and W607A mutants, but not the W607Y and W607F mutants, decreased Ba2+ permeability of NMDA channels. Thus, residue NR2B(W607) may be involved in binding of divalent cations, in particular Mg2+, through a cation-pi interaction with the electron-rich aromatic ring of the tryptophan. We previously suggested that NR2B(W607) may contribute to the narrow constriction of the NMDA channel. A model is now proposed in which the M2 loop of NR2B is folded in such a way that NR2B(W607) is positioned at the narrow constriction, at a level similar to NR2B(N616) and NR1(N616), with these three residues forming a binding site for Mg2+.
A simple high-yield preparation of alkynylphosphonium triflates (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA simple high-yield preparation of alkynylphosphonium triflatesPeter J. Stang and Charles M. CrittellCite this: J. Org. Chem. 1992, 57, 15, 4305–4306Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://pubs.acs.org/doi/10.1021/jo00041a048https://doi.org/10.1021/jo00041a048research-articleACS PublicationsRequest reuse permissionsArticle Views347Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Degradation of Cholesterol in Egg Yolk by Rhodococcus equi No. 23 (1988)

ABSTRACT The degradation of egg yolk cholesterol was investigated with Rhodococcus equi No. 23, a strain previously isolated from butter. R. equi No. 23 was inoculated into culture media (10 mL) containing egg yolk at various levels (0.2 to 1.4g) and grown with continuous shaking at 37°C for varying time periods. Egg yolk cholesterol was degraded via 4‐cholesten‐3‐one into nonsteroid compounds with almost no accumulation of steroid intermediates, as detected by thin‐layer chromatography.
Protein Gel Rheology (2013)

This chapter discusses the basic framework for measuring and understanding protein gel rheology. The chapter focuses on the use of shear rheology as a tool for quantifying the mechanical properties of protein gels. Many tissues undergo significant compressive or tensile deformation in addition to shear deformation. A number of recent studies have been reported utilizing compression and extensional testing to evaluate the mechanical properties of biological gels and tissues. Microrheology is a relatively new technique that utilizes embedded nano- or micron-sized particles as probes of local mechanical properties. Scanning probe microscopy (SPM) is another technique that can be used to measure the mechanics of individual fibers in protein gels.
Selective blockade of T lymphocyte K<sup>+</sup>channels ameliorates experimental autoimmune encephalomyelitis, a model for multiple sclerosis (2001)

Adoptive transfer experimental autoimmune encephalomyelitis (AT-EAE), a disease resembling multiple sclerosis, is induced in rats by myelin basic protein (MBP)-activated CD4(+) T lymphocytes. By patch-clamp analysis, encephalitogenic rat T cells stimulated repeatedly in vitro expressed a unique channel phenotype ("chronically activated") with large numbers of Kv1.3 voltage-gated channels (approximately 1500 per cell) and small numbers of IKCa1 Ca(2+)-activated K(+) channels (approximately 50-120 per cell). In contrast, resting T cells displayed 0-10 Kv1.3 and 10-20 IKCa1 channels per cell ("quiescent" phenotype), whereas T cells stimulated once or twice expressed approximately 200 Kv1.3 and approximately 350 IKCa1 channels per cell ("acutely activated" phenotype). Consistent with their channel phenotype, [(3)H]thymidine incorporation by MBP-stimulated chronically activated T cells was suppressed by the peptide ShK, a blocker of Kv1.3 and IKCa1, and by an analog (ShK-Dap(22)) engineered to be highly specific for Kv1.3, but not by a selective IKCa1 blocker (TRAM-34). The combination of ShK-Dap(22) and TRAM-34 enhanced the suppression of MBP-stimulated T cell proliferation. Based on these in vitro results, we assessed the efficacy of K(+) channel blockers in AT-EAE. Specific and simultaneous blockade of the T cell channels by ShK or by a combination of ShK-Dap(22) plus TRAM-34 prevented lethal AT-EAE. Blockade of Kv1.3 alone with ShK-Dap(22), but not of IKCa1 with TRAM-34, was also effective. When administered after the onset of symptoms, ShK or the combination of ShK-Dap(22) plus TRAM-34 greatly ameliorated the clinical course of both moderate and severe AT-EAE. We conclude that selective targeting of Kv1.3, alone or with IKCa1, may provide an effective new mode of therapy for multiple sclerosis.
The efficacy of lamotrigine in the management of chemotherapy-induced peripheral neuropathy: A phase III randomized, double blind, placebo-controlled NCCTG trial, N01C3 (2006)

8530 Background: Chemotherapy-induced peripheral neuropathy (CIPN) is a common dose limiting complication of chemotherapy. Lamotrigine (an anti-convulsant drug) appears to have some benefit when used to treat pain from neuropathy due to various etiologies. Based on these data, we conducted a phase III randomized placebo controlled study to determine the efficacy of lamotrigine in treating symptoms from CIPN. Methods: Patients with CIPN for 1 month or more were randomly assigned to lamotrigine (target dose 300 mg/day, increased by 50 mg/2 weeks) or placebo for 10 weeks. The co-primary endpoints were differences in average CIPN symptoms assessed by pro-rated area under the curve (AUC) of a numerical pain rating scale (NRS) and the Eastern Cooperative Oncology Group sensory neuropathy (ESN) scale. Accrual of 60 patients per group provided t-tests with 80% power to detect a difference of 0.57 standard deviation for each co-primary endpoint (moderate effect size) with a type I error of 0.025. Results: 131patients were enrolled, with complete data available for analysis in 93 (72%). Analyses were limited to this cohort but will be updated. Chemotherapy drugs considered to be causative of the CIPN were vinca alkaloids (30%), taxanes (25%), platinum-agents (7%), combinations (34%), and others (3%). Patients were equally matched with regards to baseline characteristics. Patients who enrolled had severe symptoms, with a baseline median ESN at enrollment of 3 (out of 3) in both groups. Toxicities were similar in both groups, however, more patients discontinued lamotrigine due to toxicities/refusals than those on placebo (32% vs 13% resp; p=0.04) and were therefore less likely to complete the 10 weeks therapy (60% vs. 78% resp, p=0.08). Average AUC of NRS score for patients on lamotrigine and placebo was 30.5 and 33.7 resp (p=0.48). The corresponding average AUC of the ESN scores were 12.4 and 14.5 (p=0.23). The proportions of patients with a 10 point (of 100) improvement in worst pain score (39% vs 36%) and a 1 point change in ESN (25% vs 27%) were similar between the lamotrigine and placebo arms, resp. Conclusions: These results suggest that lamotrigine is not effective for managing pain and symptoms due to CIPN. No significant financial relationships to disclose.
Treating cell culture media with UV irradiation against adventitious agents: Minimal impact on CHO performance (2014)

Sterility of cell culture media is an important concern in biotherapeutic processing. In large scale biotherapeutic production, a unit contamination of cell culture media can have costly effects. Ultraviolet (UV) irradiation is a sterilization method effective against bacteria and viruses while being non-thermal and non-adulterating in its mechanism of action. This makes UV irradiation attractive for use in sterilization of cell culture media. The objective of this study was to evaluate the effect of UV irradiation of cell culture media in terms of chemical composition and the ability to grow cell cultures in the treated media. The results showed that UV irradiation of commercial cell culture media at relevant disinfection doses impacted the chemical composition of the media with respect to several carboxylic acids, and to a minimal extent, amino acids. The cumulative effect of these changes, however, did not negatively influence the ability to culture Chinese Hamster Ovary cells, as evaluated by cell viability, growth rate, and protein titer measurements in simple batch growth compared with the same cells cultured in control media exposed to visible light.
X-Ray structure, conformation, and absolute configuration of 8-bromotetra-O-methyl-(+)-catechin (1978)

The X-ray structure of 8-bromotetra-O-methyl-(+)-catechin confirms the substitution position of the bromine atom and the 2R,3S absolute configuration of the parent compound (+)-catechin; in the heterocyclic ring both the heteroatom and C-4 are marginally above the mean plane of the adjoining benzene ring, and its conformation is midway between half-chair and sofa arrangements.
DESIGN OF VCO USING CURRENT MODE LOGIC WITH LOW SUPPLY SENSITIVITY (2014)

The function of an oscillator is to convert dc energy into RF. Voltage Controlled Oscillator (VCO) is a type of oscillator whose output frequency can be varied by a control voltage. Here the VCO is designed using 0.18m CMOS-RF technology using current mode logic. The Current mode logic is the fastest logic and is mainly used in Clock and Data Recovery applications. Current mode logic (CML) is a differential logic and provides noise immunity. Using the proposed design the current mode logic VCO operates with a supply voltage of 1.8 V and frequency varies from 2 MHz to 2.5 MHz. Its normalized supply sensitivity is 0.976 and optimum sensitivity is 0.966.
Construction and Characterization of Adenovirus Vectors (2009)

INTRODUCTION Genetically modified adenoviruses (Ads) make attractive vectors for the delivery of exogenous DNA to mammalian cells for basic science and gene therapy applications. Ad vector production consists of (1) cloning a transgene into an infectious plasmid by in vivo recombination in bacteria, (2) rescuing and propagating the vector in complementing cells, and (3) purifying the vector. All of this can be accomplished using commercially available reagents, plasmids, and cell lines. First-generation Ads have a large cloning capacity (5-14 kbp) and efficiently transduce a wide range of both quiescent and proliferating cell types. They are readily propagated to produce high-titer stocks (10 11 -10 13 vector particles from a 3-L culture). Furthermore, Ads rarely integrate into the host genome and are relatively safe. However, Ad vector production typically takes 4-6 wk, and promiscuous host-cell transduction can occur in vivo. Furthermore, immune responses against viral proteins encoded by the vector backbone can occur, which limits the duration of transgene expression in vivo. Regardless of these limitations, Ad remains one of the more versatile and efficient gene delivery systems. Here, we discuss methods for the generation, propagation, purification, and characterization of first-generation Ad vectors.
Optimal Sizing of a Dynamic Wireless Power Transfer System for Highway Applications (2018)

Inductive power transfer has been proposed as a solution to power future automated and electrified highways. In this study, an interoperable wireless charging system is sized so that a light and a heavy-duty vehicle can travel at or near charge-sustaining mode at high speeds using an optimization approach. The conflicting objectives of minimizing the power ratings and the number of inverters, coupler materials, and overall system coverages result in a Pareto Front that is presented in this paper. It is found that a system using short transmitting couplers can ensure high efficiency power transfers to light-duty vehicles (LDVs) and still maintain charge-sustaining operation of heavy-duty vehicles (HDVs). The findings are contextualized by a brief discussion of other aspects relating to the implementation of this technology on roadways such as the impact of the cost of time and travel speeds.
Efficient solutions to the replicated log and dictionary problems (1986)

We propose efficient algorithms to maintain a replicated dictionary using a log in an unreliable network. A non-serializable approach is used to achieve high concurrency. The solutions are resilient to both node and communication failures. Optimizations are developed for networks which are not completely connected.
A statistical model for the MM-wave indoor radio channel (2003)

A statistical multipath model is presented to characterize the multipath propagation properties of indoor radio channels which operate in the MM-wave frequency band. The model is derived from measurement data obtained in various typical indoor environments. The results of the statistical modeling agree closely to a discrete uncorrelated scattering model. The proposed model fits measurements well, is simple to use in performance analysis and simulation studies of MM-wave indoor radio links and is extendable to other environments.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Evolution of a reactive environment (1995)

A basic tenet of "Ubiquitous computing" (Weiser, 1993 [13]) is that technology should be distributed in the environment (ubiquitous), yet invisible, or transparent. In practice, resolving the seeming paradox arising from the joint demands of ubiquity and transparency is less than simple. This paper documents a case study of attempting to do just that. We describe our experience in developing a working conference room which is equipped to support a broad class of meetings and media. After laying the groundwork and establishing the context in the Introduction, we describe the evolution of the room. Throughout, we attempt to document the rationale and motivation. While derived from a limited domain, we believe that the issues that arise are of general importance, and have strong implications on future research.