gebhart/patrepeval huggingface dataset:
MICROSTRUCTURE OF CLAY-POLYMER COMPOSITES (2000)

X-ray diffraction has been utilized to characterize basal plane spacing changes in Laponite RDS, a synthetic smectite-type clay, due to insertion of polymer species between the clay silicate sheets. To establish the effect of polymer intercalation on the basal plane spacing of Laponite RDS, two clay-polymer composite case studies are described: Laponite RDS and a polyester ionomer AQ55D, and Laponite RDS and a biopolymer gelatin. With polymer addition, Laponite RDS basal plane spacings ranged from 12.4 to -40 A depending upon the polymer, polymer weight fraction, and ambient humidity.
Integrating UPnP in a development environment for service-oriented applications (2008)

Composing software services is a challenging activity that requires solving both low-level technical problems and high-level semantic issues. In this paper, we present a tool allowing service composition within domains. A service composition is described with a model description language with domain concepts. The tool integrates multiples technologies like UPnP or DPWS to automate the development of service composition for a developer without any specific technology knowledge. This environment has been used successfully for the development of Internet gateways in the building computing field.
IpaD Localizes to the Tip of the Type III Secretion System Needle of <i>Shigella flexneri</i> (2006)

Shigella flexneri, the causative agent of shigellosis, is a gram-negative bacterial pathogen that initiates infection by invading cells within the colonic epithelium. Contact with host cell surfaces induces a rapid burst of protein secretion via the Shigella type III secretion system (TTSS). The first proteins secreted are IpaD, IpaB, and IpaC, with IpaB and IpaC being inserted into the host cell membrane to form a pore for translocating late effectors into the target cell cytoplasm. The resulting pathogen-host cross talk results in localized actin polymerization, membrane ruffling, and, ultimately, pathogen entry. IpaD is essential for host cell invasion, but its role in this process is just now coming to light. IpaD is a multifunctional protein that controls the secretion and presentation of IpaB and IpaC at the pathogen-host interface. We show here that antibodies recognizing the surface-exposed N terminus of IpaD neutralize Shigella's ability to promote pore formation in erythrocyte membranes. We further show that MxiH and IpaD colocalize on the bacterial surface. When TTSS needles were sheared from the Shigella surface, IpaD was found at only the needle tips. Consistent with this, IpaD localized to the exposed tips of needles that were still attached to the bacterium. Molecular analyses then showed that the IpaD C terminus is required for this surface localization and function. Furthermore, mutations that prevent IpaD surface localization also eliminate all IpaD-related functions. Thus, this study demonstrates that IpaD localizes to the TTSA needle tip, where it functions to control the secretion and proper insertion of translocators into host cell membranes.
Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices (2004)

We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead neurons were labeled with a membrane-impermeant fluorescent DNA-binding dye (Sytox Orange or To-Pro-3). Tissue injury during the slicing procedure induced neuronal death and microglial activation, but the density of dead cells diminished approximately 10-fold by 7 days in vitro as resident microglia cleared dead cells. In time-lapse movies (4-20 h long), activated microglia exhibited varying levels of motile and locomotory activity. The motility of microglia could change abruptly following contact by other microglia or death of nearby cells. When neighboring cells died, some microglia rapidly moved toward or extended a process to engulf the dead cell, consistent with a chemotactic signaling response. Dead cell nuclei usually were engulfed and carried along by highly motile and locomoting microglia. The mean time to engulfment was approximately 5 times faster for newly deceased cells (33 min) than for extant dead cells (160 min), suggesting that the efficacy of microglial phagocytosis in situ might vary with time after cell death or mode of cell death. These observations demonstrate that activated microglia are heterogeneous with respect to motile activity following traumatic tissue injury and further indicate that cell motility in situ is temporally regulated at the single cell level, possibly by direct cell-cell contact and by diffusible substances emanating from nearby dead cells.
Suppressive effect of tritoqualine (TRQ) on the acceleration of fibrosis in the liver. (1986)

Liver cirrhosis was induced by consecutive CCl4-treatment of rats (0.5 ml/kg, s.c., 2 times/week) to investigate the effect of TRQ on the acceleration of fibrosis in the liver. An increase of hydroxyproline content in the liver of rats began 12 weeks after the CCl4 treatment and a 1.9-fold increase was observed at week 14 compared with non-CCl4 treated rats. Histamine in the liver increased about 2 times at week 14. Increased numbers of mast cells were seen in the area of proliferated collagen fiber in the liver under microscopic observation, and also a good correlation was recognized between the number of mast cells and the progression of fibrosis. An administration of TRQ to the rats for 2 weeks from week 13 resulted in significant suppression of both the increase in hydroxyproline and histamine in the liver dose-dependently compared with the CCl4 control group. Both progression of collagen and increase in mast cell numbers were also suppressed by TRQ dose-dependently under histopathological observation; at the same time the decrease in mast cells was recognized to correspond to the decrease in hydroxyproline and histamine in the liver. Thus, it was suggested that increased mast cells participated in the biosynthesis of collagen. Though the elevated serum transaminases, alkaline phosphatase and leucine amino peptidase were also suppressed by TRQ administration, the protein biosynthesis activity of the liver and lowered serum total cholesterol were not improved as much as the other parameters. From these results, it was shown that TRQ was especially and remarkably effective in suppressing the acceleration of fibrosis, and one of the pharmacological mechanisms of this action may be ascribed to the inhibitory effect of TRQ on the activation of mast cells by some stimulants.
L-deprenyl potentiates NGF-induced changes in superoxide dismutase mRNA in PC12 cells (1998)

L-deprenyl protects neurons in a number of in vivo and in vitro models and it has been postulated that it ameliorates some neurodegenerative disorders. Superoxide dismutase (SOD) is one of the enzymes responsible for the inactivation of oxygen free radicals, and one of the mechanisms of the effect of L-deprenyl is thought to act by induction of SOD. In this study, PC12 cells were used to study the effect of L-deprenyl on gene regulation of SOD and its interaction with nerve growth factor (NGF). The results show that NGF induces SOD mRNA in a dose-dependent manner and that a similar effect was produced by L-deprenyl. In addition, L-deprenyl potentiates NGF effects. This study demonstrates that both L-deprenyl and NGF may be involved in common antioxidative mechanisms and that L-deprenyl may interact with neurotrophic factors.
Genetic, metabolic and environmental factors involved in the development of liver cirrhosis in Mexico (2015)

Liver cirrhosis (LC) is a chronic illness caused by inflammatory responses and progressive fibrosis. Globally, the most common causes of chronic liver disease include persistent alcohol abuse, followed by viral hepatitis infections and nonalcoholic fatty liver disease. However, regardless of the etiological factors, the susceptibility and degree of liver damage may be influenced by genetic polymorphisms that are associated with distinct ethnic and cultural backgrounds. Consequently, metabolic genes are influenced by variable environmental lifestyle factors, such as diet, physical inactivity, and emotional stress, which are associated with regional differences among populations. This Topic Highlight will focus on the genetic and environmental factors that may influence the metabolism of alcohol and nutrients in the setting of distinct etiologies of liver disease. The interaction between genes and environment in the current-day admixed population, Mestizo and Native Mexican, will be described. Additionally, genes involved in immune regulation, insulin sensitivity, oxidative stress and extracellular matrix deposition may modulate the degree of severity. In conclusion, LC is a complex disease. The onset, progression, and clinical outcome of LC among the Mexican population are influenced by specific genetic and environmental factors. Among these are an admixed genome with a heterogenic distribution of European, Amerindian and African ancestry; a high score of alcohol consumption; viral infections; a hepatopathogenic diet; and a high prevalence of obesity. The variance in risk factors among populations suggests that intervention strategies directed towards the prevention and management of LC should be tailored according to such population-based features.
Immunohistochemical Analysis of Human Growth Hormone-Releasing Hormone Gene Expression in Transgenic Mice* (1989)

The tissue-specific expression of a fusion gene encoding the mouse metallothionein-1 promoter and the coding region of the human GH-releasing hormone (hGRH) gene was studied in transgenic mice by immunohistochemistry using an anti-hGRH serum that does not recognize endogenous mouse GRH. hGRH immunoreactivity (GRH-IR) was detected in specific cells of the pituitary, pancreas, kidney, duodenum, lung, testis, ovary, adrenal, heart, and brain. In the pituitary, using double immunofluorescent staining, GRH-IR was found in some, but not all, somatotrophs, gonadotrophs, thyrotrophs, and mammotrophs. GRH-IR was found in both pancreatic exocrine cells and endocrine islets. Within the islet, GRH-IR was colocalized in A and D cells with glucagon and somatostatin, respectively. Immunopositive cells in other tissues were localized in kidney proximal convoluted tubules, duodenal submucosal glands of Brunner, the smooth muscles of pulmonary arterioles, testicular Leydig cells, oocytes, adrenal medullary chromaffin cells, and cardiac atria. In the brain, GRH-IR was seen in the external layer of the median eminence and in perikarya and fibers of the hypothalamic arcuate nucleus, the parvocellular region of the paraventricular nucleus, the supraoptic nucleus, and the amygdala. Somatostatin-immunoreactive cell bodies and fibers in transgenic and control mouse hypothalamus were not appreciably different. In summary, hGRH expression in transgenic mice occurs in a cell-specific manner in the hypothalamus as well as in numerous other tissues, many of which have secretory functions.
Crisis and Revolution (1997)

In the late summer of 1914 Karl Kautsky, the chief theoretician of the German Social Democratic Party (SPD), the largest formal Marxist party in the world, predicted three possible outcomes of the war that had just begun: the rise of the USA to global domination; the launch of a great anticolonial struggle and the transformation of tsarist Russia (Kautsky, 1914a, p. 845; 1914b, pp. 973–4). In time all three forecasts proved correct, although the first two needed another global conflict a generation later to realise them fully. In combination, they would contribute after 1945 to the impetus that would give birth, not to the Europe of fraternal workers’ states that Kautsky sought, but a European Union of European capitalist states. This study will focus on the period from 1914 to the coming to power of Adolf Hitler’s Nazi movement in 1933, and then unevenly, but its perspective and rationale extend to the present.
High affinity interaction between C4b-binding protein and vitamin K-dependent protein S in the presence of calcium. Suggestion of a third component in blood regulating the interaction. (1990)

The anticoagulant vitamin K-dependent protein S interacts with the complement regulatory protein C4b-binding protein (C4BP), both in purified systems and in plasma. The concentrations of these proteins in plasma are approximately equimolar (0.3 microM) and 30-40% of protein S in plasma is found in the noncomplexed state. Only the uncomplexed form of protein S displays anticoagulant activity and studies have shown that patients with a selective deficiency of free protein S have a high incidence of thrombosis. In this study, we report that the protein S-C4BP interaction is at least 100-fold tighter in the presence of Ca2+ than in EDTA. The KD in the presence of Ca2+ was estimated with a gel filtration technique to be less than 5 x 10(-10) M, whereas in the presence of EDTA, it was approximately 100-fold higher. Ca2+ titration experiments suggested that the Ca2+ sites which function in the protein S-C4BP interaction are of high affinity which, in turn, suggests that they may be independent of the gamma-carboxyglutamic acid region and may be present in the epidermal growth factor-like domains of protein S. The high affinity of the protein S-C4BP interaction in the presence of Ca2+ suggested that virtually all of the protein S in whole blood should be complexed with C4BP. However, even though the protein S-C4BP interaction in Ca2(+)-containing serum was shown to have the same high affinity as in purified systems, approximately 30-40% of the protein S in serum was free. These results appear best explained by the presence of a third component in whole blood which regulates the protein S-C4BP interaction, keeping approximately 30-40% of circulating protein S in its free, functionally anticoagulant form. It is speculated that persons with little free protein S may be deficient in this hypothetical third component.
An Evaluation of the Quantitative Relationship between the Subjective Stress Value and Heart Rate Variability (2000)

We evaluated the quantitative relationship between the subjective stress value and heart rate variability. The mean values of R-R interval (RRI) and respiratory sinus arrhythmia (RSA), which were reported to be markedly affected by stress, were used as heart rate variability. The following mental workload was performed so as to give wide range loads to the subjects: presentation of research, asking questions in research presentation, research presentation practice, and supine rest. The subjective stress value was obtained as serial values from 1 to 5 by a newly-developed questionnaire. The correlation between the stress value and RRI or RSA when such wide range workload are given was evaluated. There was a high correlation between the subjective stress value and RRI (correlation coefficient, -0.637), but not between the subjective stress value and RSA (coefficient, -0.319) Signal processing of RRI was simpler than that of RSA. These results suggest the usefulness of RRI as an index of the subjective stress value.
Detection of <i>Legionella pneumophila</i> Using a Real-Time PCR Hybridization Assay (2000)

A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.
Collimated sputter deposition, a novel method for large area deposition of Spindt type field emission tips (1995)

A new technique has been developed to manufacture Spindt type field emission (FE) cathodes. It is called collimated sputter deposition (CSD). A standard sputter deposition machine can be utilized to which a direction selecting filter is added. The particles passing the collimator are incident on the integrated field emission structure. As a result the holes in the gate are closed and tips are formed. A number of collimator configurations has been considered. The influence of the dominating process parameters is investigated. Several tip materials have been tested. FE results on tips manufactured by means of CSD are presented.
Decrease in phosphorylated ERK indicates the therapeutic efficacy of a clinical PI3Kα-selective inhibitor CYH33 in breast cancer (2018)

PI3Ks are frequently hyper-activated in breast cancer and targeting PI3Kα has exhibited promising but variable response in preclinical and clinical settings. CYH33 is a novel PI3Kα-selective inhibitor in phase I clinical trial. We investigated the efficacy of CYH33 against breast cancer and explored potential predictive biomarkers. CYH33 potently restrained tumor growth in mice bearing human breast cancer cell xenografts and in R26-Pik3ca<sup>H1047R</sup>;MMTV-Cre transgenic mice. CYH33 significantly inhibited proliferation of a panel of human breast cancer cells, while diversity in sensitivity has been observed. Cells harboring activating PIK3CA mutation, amplified HER2 were more responsive to CYH33 than their counterparts. Besides, cells in HER2-enriched or luminal subtype were more sensitive to CYH33 than basal-like breast cancer. Sensitivity to CYH33 has been further revealed to be associated with induction of G1 phase arrest and simultaneous inhibition of Akt and ERK. Sensitivity of patient-derived xenograft to CYH33 was also positively correlated with decrease in phosphorylated ERK. Taken together, CYH33 is a promising PI3Kα inhibitor for breast cancer treatment and decrease in ERK phosphorylation may indicate its efficacy, which provides useful clues for rational design of the ongoing clinical trials.
Mixed Self-Assembled Multilayer of 4,4‘-Dimercaptobiphenyl and 1,8-Octanedithiol (2003)

Mixed multilayers from aromatic (biphenyl) and aliphatic (octane) dithiols, using Cu(II) ions, have been prepared and characterized by ellipsometry, FTIR, and XPS. The data support the formation of multilayers in which interlayer disulfide linkages connect the 4,4‘-dimercaptobiphenyl layers and intralayer disulfide bonds connect 1,8-octanedithiol molecules. Cu(I) ions connect the aromatic and aliphatic layers.
Anomalous Ti SALICIDE gate to source/drain shorts induced by dry Si etch during TiSi/sub 2/ local interconnect formation (1994)

A new failure mode was observed in a 0.5 μm version of the silicided amorphous-silicon contact and interconnect (SAC) technology. Massive PMOS gate to source/drain shorts were found. The cause is attributed to formation of Ti during the Si etch. The fluorinated Ti surface fails to form adequate TiN diffusion barrier during subsequent rapid thermal annealing (RTA) in N <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> or NH <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> ambient. Si diffuses from the polycrystalline Si gate and/or the p-type source/drain onto the spacer, reacts with Ti and forms resistive leakage paths. A blanket low-dose, low-energy As implant prior to Ti deposition corrects this problem without adversely changing device characteristics.
Step-by-Step Engineered Multiparticle Entanglement (2000)

After quantum particles have interacted, they generally remain in an entangled state and are correlated at a distance by quantum-mechanical links that can be used to transmit and process information in nonclassical ways. This implies programmable sequences of operations to generate and analyze the entanglement of complex systems. We have demonstrated such a procedure for two atoms and a single-photon cavity mode, engineering and analyzing a three-particle entangled state by a succession of controlled steps that address the particles individually. This entangling procedure can, in principle, operate on larger numbers of particles, opening new perspectives for fundamental tests of quantum theory.
Architectural tradeoffs for survivor sequence memory management in Viterbi decoders (1993)

In a Viterbi decoder, there are two known memory organization techniques for the storage of survivor sequences from which the decoded information sequence is retrieved, namely, register exchange method and traceback method. This work extends previously known traceback approaches, describes two new traceback algorithms, and compares various traceback methods with each other. Memory size, latency, and implementational complexity of the survivor sequence management are analyzed for both uniprocessor and multiprocessor realizations of Viterbi decoders. A new, one-pointer traceback method is shown to be better than previously known traceback methods.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pulsed‐field Gel Electrophoresis of DNA (2003)

Abstract Conventional electrophoresis is not useful for DNA larger than 50 000 base pairs (bp). For large DNA molecules up to 7 million bp (7 Mb) it is necessary to use pulsed‐field electrophoresis. This protocol describes how the procedure is carried out.
MICROCHANNEL DEVICES FOR EFFICIENT CONTACTING OF LIQUIDS IN SOLVENT EXTRACTION (1999)

Microchannel devices were designed and tested for efficient contacting of two liquids in solvent extraction, and the results are presented. This study is part of an overall effort to produce and demonstrate efficient compact devices for chemical separations. Engineering these devices at the microscale offers many technical advantages. Achieving high contact area per unit system volume, thin-film contacting, and establishing uniform flow distribution result in substantially higher throughput per total system volume over conventional technologies. Theoretical calculations are presented that provide insight into the relative importance of various resistances to mass transfer, as well as their relationship to overall performance of the microchannel devices. Experimental results are presented for device performance using both commercial polymeric membranes and micromachined contactor plates for stabilizing the liquid-liquid interface. These results indicate that current-generation micromachined plates perform as well as commercial membranes, with substantial potential for improvement.
Characterization of human residual catalase of an acatalasemic patient by isoelectric focusing and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis followed by electrophoretic blotting and immunodetection (1989)

Isoelectric points and subunit sizes of catalases in human blood and human cultured skin fibroblasts from acatalasemic and normal subjects were analyzed by isoelectric focusing in agarose gel and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively, followed by electroblotting to polyvinylidene difluoride membranes for immunodetection. The results indicated that the isoelectric point of residual catalase in the C fraction prepared from acatalasemic erythrocytes was identical with that of catalase prepared from normal erythrocytes. The residual catalase in homogenates of acatalasemic cultured skin fibroblasts also reacted with anticatalase rabbit serum and had an isoelectric point identical with that of normal catalase. Subunit sizes of normal and acatalasemic catalases in the C fractions of erythrocytes were also found to be identical on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by electroblotting and immunoenzymatic amplification. The results indicated no substantial difference in molecular size and charge of catalase proteins between normal and acatalasemic erythrocytes and fibroblasts.