gebhart/patrepeval huggingface dataset:
LPIN1 gene mutations: a major cause of severe rhabdomyolysis in early childhood (2010)

Autosomal recessive LPIN1 mutations have been recently described as a novel cause of rhabdomyolysis in a few families. The purpose of the study was to evaluate the prevalence of LPIN1 mutations in patients exhibiting severe episodes of rhabdomyolysis in infancy. After exclusion of primary fatty acid oxidation disorders, LPIN1 coding sequence was determined in genomic DNA and cDNA. Among the 29 patients studied, 17 (59%) carried recessive nonsense or frameshift mutations, or a large scale intragenic deletion. In these 17 patients, episodes of rhabdomyolysis occurred at a mean age of 21 months. Secondary defect of mitochondrial fatty oxidation or respiratory chain was found in skeletal muscle of two patients. The intragenic deletion, c.2295-866_2410-30del, was identified in 8/17 patients (47%), all Caucasians, and occurred on the background of a common haplotype, suggesting a founder effect. This deleted human LPIN1 form was unable to complement Delta pah1 yeast for growth on glycerol, in contrast to normal LPIN1. Since more than 50% of our series harboured LPIN1 mutations, LPIN1 should be regarded as a major cause of severe myoglobinuria in early childhood. The high frequency of the intragenic LPIN1 deletion should provide a valuable criterion for fast diagnosis, prior to muscle biopsy.
Engineered Proteinticles for Targeted Delivery of siRNA to Cancer Cells (2015)

Considering the problems of small interfering RNA (siRNA) delivery using traditional viral and nonviral vehicles, a new siRNA delivery system to enhance efficiency and safety needs to be developed. Here human ferritin‐based proteinticles are genetically engineered to simultaneously display various functional peptides on the surface of proteinticles: cationic peptide to capture siRNA, tumor cell targeting and penetrating peptides, and enzymatically cleaved peptide to release siRNA inside tumor cell. In the in vitro treatment of poly‐siRNA‐proteinticle complex, both of the tumor cell targeting and penetrating peptides are important for efficient delivery of siRNA, and the red fluorescent protein (RFP) expression in RFP‐expressing tumor cells is notably suppressed by the delivered siRNA with the complementary sequence to RFP mRNA. It seems that the human ferritin‐based proteinticle is an efficient, stable, and safe tool for siRNA delivery, having a great potential for application to in vivo cancer treatment. The unique feature of proteinticles is that multiple and functional peptides can be simultaneously and evenly placed and also easily switched on the proteinticle surface through a simple genetic modification, which is likely to make proteinticles appropriate for targeted delivery of siRNA to a wide range of cancer cells.
GC-Profile: a web-based tool for visualizing and analyzing the variation of GC content in genomic sequences (2006)

In order to understand the evolution, structure and function of genomes, it is important to know the general compositional features of DNA sequences. Based on the quadratic divergence, a new segmentation algorithm to partition a given genome or DNA sequence into compositionally distinct domains has been put forward. With the aid of the technique of cumulative GC profile, the distribution of segmentation points can be displayed intuitively. We have therefore developed them into GC-Profile, an interactive web-based software system, which can be used to segment prokaryotic and eukaryotic genomes. GC-Profile provides a quantitative and qualitative view of genome organization. Based on the obtained results, the relationships between the G+C content and other genomic features, such as distributions of genes and CpG islands, can be analyzed in a perceivable manner. It shows that GC-Profile would be an appropriate starting point for analyzing the isochore structure of higher eukaryotic genomes, and an intuitive tool for identifying genomic islands in prokaryotic genomes. GC-Profile is freely available at the website http://tubic.tju.edu.cn/GC-Profile/. In addition, precompiled binaries, together with examples and documentation, can also be freely downloaded for a local execution.
Using AVO for fracture detection: Analytic basis and practical solutions (1997)

Tight, low-porosity reservoirs can produce significant amounts of hydrocarbons if fracture networks provide the necessary permeability. Characterization of naturally fractured reservoirs using surface seismic data is an important exploration problem that has attracted much attention in the literature. TLE, for example, had an entire issue (August 1996) on this subject. While the data and modeling in existing publications demonstrate that fracturing causes significant azimuthal variation in recorded seismic signatures, the problem is how to infer fracture parameters from these data.
Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components (2007)

Two forms of RNA Polymerase IV (PolIVa/PolIVb) have been implicated in RNA-directed DNA methylation (RdDM) in Arabidopsis. Prevailing models imply a distinct function for PolIVb by association of Argonaute4 (AGO4) with the C-terminal domain (CTD) of its largest subunit NRPD1b. Here we show that the extended CTD of NRPD1b-type proteins exhibits conserved Argonaute-binding capacity through a WG/GW-rich region that functionally distinguishes Pol IVb from Pol IVa, and that is essential for RdDM. Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally conserved Argonaute-binding platforms in RNA interference (RNAi)-related components.
Effect of Chromium and Niobium Doping on the Morphology and Electrochemical Performance of High-Voltage Spinel LiNi<sub>0.5</sub>Mn<sub>1.5</sub>O<sub>4</sub> Cathode Material (2016)

Undoped, Cr-doped, and Nb-doped LiMn(1.5)Ni(0.5)O4 (LNMO) is synthesized via a PVP (polyvinylpyrrolidone)-combustion method by calcinating at 1000 °C for 6 h. SEM images show that the morphology of LNMO particles is affected by Cr and Nb doping. Cr doping results in sharper edges and corners and smaller particle size, and Nb doping leads to smoother edges and corners and more rounded and larger particles. The crystal and electron structure is investigated by XRD- and synchrotron-based soft X-ray absorption spectroscopy (sXAS). Cr doping and light Nb doping (LiNb(0.02)Ni(0.49)Mn(1.49)O4) improve the rate performance of LNMO. To explore the reason for rate-performance improvement, we conducted potential intermittent titration technique (PITT) and electrochemical impedance spectroscopy (EIS) tests. The Li(+) chemical diffusion coefficient at different state of charge (SOC) is calculated and suggests that both Cr and light Nb doping speeds up Li(+) diffusion in LNMO particles. The impedance spectra show that both R(SEI) and R(ct) are reduced by Cr and light Nb doping. The cycling performance is improved by Cr or Nb doping, and Cr doping increases both Coulombic efficiency and energy efficiency of LNMO at 1 C cycling. The LiCr(0.1)Ni(0.45)Mn(1.45)O4 remains at 94.1% capacity after 500 cycles at 1 C, and during the cycling, the Coulombic efficiency and energy efficiency remain at over 99.7% and 97.5%, respectively.
Role of<scp>l</scp>-glutamate in systemic AVP-induced hypothermia (2003)

It has been reported that systemic injection of arginine vasopressin (AVP) induces a drop in body core temperature (T(c)), but little is known about the mechanisms involved. Because glutamate is an important excitatory neurotransmitter involved in a number of thermoregulatory actions, in the present study, we tested the hypothesis that glutamate plays a role in systemic AVP-induced hypothermia. Wistar rats were pretreated intracerebroventricularly (icv) with kynurenic acid, an antagonist of l-glutamate ionotropic receptors, alpha-methyl-(4-carboxyphenyl)glycine (MCPG), an antagonist of l-glutamate metabotropic receptors, or saline 15 min before intravenous injection of AVP (2 microg/kg) or saline. T(c), brown adipose tissue (BAT) temperature, blood pressure, heart rate, and tail skin temperature were measured continuously. Administration of saline icv followed by intravenous AVP caused a significant drop in T(c) brought about by a reduction in BAT thermogenesis and an increase in heat loss through the tail. MCPG treatment (icv) did not affect the fall in T(c) induced by AVP. Treatment with kynurenic acid (icv) abolished AVP-induced hypothermia but did not affect the AVP-evoked rise in blood pressure or drop in heart rate and BAT temperature. Heat loss through the tail was significantly reduced in animals injected with AVP and pretrated with kynurenic acid. These data indicate that ionotropic receptors of l-glutamate in the central nervous system participate in peripheral AVP-induced hypothermia by affecting heat loss through the tail.
Amifostine Inhibits Hematopoietic Progenitor Cell Apoptosis by Activating NF-κB/Rel Transcription Factors (1999)

We investigated the involvement of NF-kappaB/Rel transcription factors that reportedly can inhibit apoptosis in various cell types in the antiapoptotic mechanism of the cytoprotectant amifostine. In the nontumorigenic murine myeloid progenitor 32D cells incubated with amifostine, we detected a reduction of the IkappaBalpha cytoplasmic levels by Western blotting and a raising of nuclear NF-kappaB/Rel complexes by electrophoretic mobility shift assay. Amifostine inhibited by more than 30% the growth factor deprivation-induced apoptosis, whereas its effect failed when we blocked the NF-kappaB/Rel activity with an NF-kappaB/Rel-binding phosphorothioate decoy oligodeoxynucleotide. In human cord blood CD34(+) cells, the NF-kappaB/Rel p65 subunit was detectable (using immunofluorescence analysis) mainly in the cytoplasm in the absence of amifostine, whereas its presence was appreciable in the nuclei of cells incubated with the cytoprotectant. In 4 CD34(+) samples incubated for 3 days in cytokine-deficient conditions, cell apoptosis was reduced by more than 30% in the presence of amifostine (or amifostine plus a control oligo); the effect of amifostine was abolished in cultures with the decoy oligo. These findings indicate that the inhibition of hematopoietic progenitor cell apoptosis by amifostine requires the induction of NF-kappaB/Rel factors and that the latter can therefore exert an antiapoptotic activity in the hematopoietic progenitor cell compartment. Furthermore, the identification of this specific mechanism underlying the survival-promoting activity of amifostine lends support to the possible use of this agent in apoptosis-related pathologies, such as myelodysplasias.
Strategies for gallium removal after focused ion beam patterning of ferroelectric oxide nanostructures (2007)

As part of a study into the properties of ferroelectric single crystals at nanoscale dimensions, the effects that focused ion beam (FIB) processing can have, in terms of structural damage and ion implantation, on perovskite oxide materials has been examined, and a post-processing procedure developed to remove such effects. Single crystal material of the perovskite ferroelectric barium titanate (BaTiO(3)) has been patterned into thin film lamellae structures using a FIB microscope. Previous work had shown that FIB patterning induced gallium impregnation and associated creation of amorphous layers in a surface region of the single crystal material some 20 nm thick, but that both recrystallization and expulsion of gallium could be achieved through thermal annealing in air. Here we confirm this observation, but find that thermally induced gallium expulsion is associated with the formation of gallium-rich platelets on the surface of the annealed material. These platelets are thought to be gallium oxide. Etching using nitric and hydrochloric acids had no effect on the gallium-rich platelets. Effective platelet removal involved thermal annealing at 700 degrees C for 1 h in a vacuum followed by 1 h in oxygen, and then a post-annealing low-power plasma clean in an Ar/O atmosphere. Similar processing is likely to be necessary for the full recovery of post FIB-milled nanostructures in oxide ceramic systems in general.
Response of osteosarcoma and Ewing sarcoma to preoperative chemotherapy: assessment with dynamic and static MR imaging and skeletal scintigraphy. (1990)

In 15 osteosarcomas and six Ewing sarcomas, response to preoperative chemotherapy was assessed with magnetic resonance (MR) imaging without and with gadolinium diethylenetriaminepentaacetic acid (DTPA) enhancement and with dynamic Gd-DTPA studies, and the results were compared with the scintigraphic findings. All studies were obtained prior to and following preoperative chemotherapy. Static MR imaging was of little value for assessment of response; reduction in signal intensity within soft-tissue masses on the T2-weighted spin-echo images indicated response with a sufficient degree of accuracy (71%) but low sensitivity, whereas an increase in signal intensity after Gd-DTPA administration indicated zones of viable tissue with low specificity. With three-phase skeletal scintigraphy, the findings in the perfusion and blood-pool phases were of no value, whereas the findings in the osseous phase allowed the prediction of response with an accuracy of 73.7%. Of all techniques employed, dynamic MR imaging had the highest degree of accuracy (85.7%) and was superior to scintigraphy, particularly in patients who were receiving intraarterial chemotherapy.
Primary Structure of the Porcine 89-kDa Enamelin (1996)

The primary structure of the 89-kDa enamelin found in porcine secretory enamel at an early stage of development was investigated. The fragments of the enamelin cDNA were amplified by polymerase chain-reaction from the first-strand enamelin cDNA, and were sequenced. The results indicated that the 89-kDa enamelin consisted of 627 amino acid residues and had a molecular mass of 70,448. A hydrophobic domain is located in the region of the 21st-62nd amino acid residues of the molecule. Acidic domains are located in two regions of the molecule-one in the region of the 135th-238th amino acid residues and the other in the C-terminal region. A basic domain is located in the region of the 239th-360th amino acid residues. The results also indicated that the low-molecular-weight enamelins were fragments derived from a prototype enamelin.
Food Commodities, Food Processing and Cooking (1995)

To assess the natural contamination of food commodities. To outline the industrial treatment of raw and processed foods. To examine common cooking and food-holding methods used in quantity food production and to assess their effectiveness in controlling contamination and growth.
Effect of ozone and nitrogen dioxide on the permeability of bronchial epithelial cell cultures of non‐asthmatic and asthmatic subjects (2002)

These results suggest that HBECs of asthmatics may be more susceptible to the deleterious effects of these pollutants. Whether in patients with asthma the greater susceptibility of bronchial epithelial cells to O3 and NO2 contributes to the development of the disease, or is a secondary characteristic of this condition, remains to be determined.
Dominantly inherited cerebello‐olivary atrophy is not due to a mutation at the spinocerebellar ataxia‐I, machado‐joseph disease, or dentato‐rubro‐pallido‐luysian atrophy locus (1996)

The dominantly inherited ataxias are characterized both by phenotypic variability (phenotypic heterogeneity) within the same genotype and overlapping phenotypes from different genotypes (genotypic heterogeneity). Therefore it is important to characterize specific clinical-neuropathologic phenotypes as precisely as possible at the genetic level. We describe a family with dominantly inherited ataxia of late adult onset with relatively "pure" cerebellar signs. Neuropathologic examination in two individuals from this family revealed findings consistent with cerebello-olivary atrophy, suggesting that this neuropathologic phenotype many run true within f families. Mutations at the spinocerebellar ataxia-I, Machado-Joseph disease, and dentatorubropallidoluysian atrophy loci were excluded by direct DNA analysis on the leukocytes of one living affected member. Thus we provide evidence that these mutations are not responsible for this particular phenotype of dominantly inherited ataxia.
Comparison of fatty acid tracers FTHA and BMIPP during myocardial ischemia and hypoxia. (1998)

Decreased retention of both BMIPP and FTHA occurred with ischemia, despite the known differences in metabolism of the two tracers. This difference in metabolism was further highlighted in the setting of hypoxia with increased BMIPP uptake. Thus, these results suggest that uptake of both FTHA and BMIPP tracks reduction of fatty acid utilization in myocardial ischemia but fails in tracking reduction of fatty acid oxidation during hypoxia.
Effect of HCV infection on expression of several cancer-associated gene products in HCC (1999)

AIM:To study hepatocarcinogenesis of hepatitis C virus (HCV).METHODS: Expression of HCV antigens (CP10, NS3 and NS5) and several cancer-associated gene products (ras p21, c-myc, c-erbB-2, mutated p53 and p16 protein) in the tissues of hepatocellular carcinoma (HCC, n = 46) and its surrounding liver tissue were studied by the ABC(avidin-biotin complex) immunohistochemical method. The effect of HCV infection on expression of those gene products in HCC was analyzed by comparing HCV antigen positive group with HCV antigen negative group.RESULTS:Positive immunostaining with one, two or three HCV antigens was found in 20 (43.5%) cases,with either of two or three HCV antigens in 16 (34.8%) cases, and with three HCV antigens in 9 (19.6%) cases.Deletion rate of p16 protein expression in HCC with positive HCV antigen (80%, 16/20)was significantly higher than that in HCC with negative HCV antigen. Whereas no significant difference of the other gene product expression was observed between the two groups.CONCLUSION:HCV appears related to about one third of cases of HCC in Chongqing, the southwest of China, and it may be involved in hepatocarcinogenesis by inhibi ting the function of p16 gene, which acts as a negative regulator of cell cycle.
Community Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, Shenzhen, China, 2020 (2020)

Since early January 2020, after the outbreak of coronavirus infection in Wuhan, China, ≈365 confirmed cases have been reported in Shenzhen, China. The mode of community and intrafamily transmission is threatening residents in Shenzhen. Strategies to strengthen prevention and interruption of these transmissions should be urgently addressed.
The chemistry of enoxysilacyclobutanes: highly selective, uncatalyzed aldol additions (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe chemistry of enoxysilacyclobutanes: highly selective, uncatalyzed aldol additionsScott E. Denmark, Brian D. Griedel, and Diane M. CoeCite this: J. Org. Chem. 1993, 58, 5, 988–990Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/jo00057a002https://doi.org/10.1021/jo00057a002research-articleACS PublicationsRequest reuse permissionsArticle Views621Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
An instrument using variation of resistance to aid in needle tip insertion in epidural block in monkeys. (1987)

A new method is proposed to detect the entrance of a needle into the epidural space. Measuring the changes in electric resistance during the insertion of the needle through different tissues in the interspinal space can help anesthesiologists judge whether and when the needle has entered the epidural space. This preliminary, animal study showed that the proposed method performed quite satisfactorily. The electric resistance of the tissues in the interspinal space varied from 1.57 +/- 1.22 k omega to 2.93 +/- 1.14 k omega, and the resistance of the epidural space varied from 9.00 +/- 1.12 k omega to 14.64 +/- 1.25 k omega. The difference was statistically significant (p less than 0.001). This difference in the resistance can be used to verify placement of the tip of the needle in the epidural space and help anesthesiologists administer epidural anesthesia.
볼링선수들의 수용전념훈련 과정의 서술적 분석 (2014)

본 연구는 대학 볼링선수 10명을 대상으로 9회기의 수용전념훈련(ACT)을 실시하였다· 수용전념치료는 인지행동치료(cognitive behavior therapy)에서 제3의 동향인 심리치료기법이다· 1회기는 ACT 소개를 위해 음악, 아로마 등을 준비하여 상담실의 분위기를 조성하였다· 선수들은 새로운 환경과 집단상담에 대한 호기심을 가지고 1회기에 응하였다· 2회기는 마음을 열어요! 라는 주제로 인디언식 이름과 서로간의 인터뷰를 통하여 서로를 인식하였다· 3회기는 본격적인 수용전념훈련의 시작으로 좌선을 하고 지시문으로 통해 마음챙김 집중연습을 하였다· 지시문에 들어있는 알아차림 문구는 선수들이 처한 마음, 신체적감각, 감정 등을 수용하기 위한 것이다· 4회기는 인지적 탈융합을 돕기위해 시합과 불안의 융합에서 빠져나오도록 하였다· 우유와 불안을 큰소리로 혹은 이상한 리듬을 활용함으로서 탈융합에 대한 이해를 실천하였다· 5회기는 통제에 대한 새로운 대안으로서 기꺼이 경험하기를 하였다· 예를들어 분홍코끼리 생각안하기를 통하여 회피의 어려움과 무의미를 경험하게 하였다· 6회기는 수행습관, 성공과 실패시의 반응을 통하여 개념화된 자기를 이해하고 언어의 융합에 의한 고정된 감정패턴에 거리두기를 시도하였다· 7회기는 가치 선택하기 과정으로 수행묘비문작성으로 장례식 모습에서 어떤 선수로 기억되고 싶은가에 대한 질문을 통하여 선수와 삶의 가치를 기술하였다· 8회기는 가치추구에 전념하는 것의 어려움과 그 장애물과 마주치게 하는 회기이다· 30초간 눈 맞추기 연습을 통하여 매우 쉽거나 어려운 상황에서 아주 작은 장애에 얼마나 쉽게 전념이 방해 받는지를 경험하게 하였다· 9회기는 프로그램 평가지를 통하여 훈련과정에서 가장 기억에 남는 내용과 그 이유, 훈련으로 인하여 변화된 점과 그 변화의 만족정도, 그리고 그 이유를 기술하였다· 이러한 집단상담에 의한 ACT를 통해 선수들은 시합과 훈련에서 알아차림과 수용, 탈융합, 목표, 전념 등을 적용하고자 노력하는 것을 알 수 있었다·
Sensorless vector-controlled induction motor drive system with rotor resistance estimation using parallel processing with floating point DSP (2002)

The paper deals with the aspects related to simultaneous rotor speed estimation and rotor resistance identification in field-oriented induction motor systems using only the induction motor model. A statistical based computing algorithm is proposed in order to avoid the division by zero. Three basic algorithms have been considered in order to establish the optimum solution. The study of the speed and rotor resistance estimator has been performed together with a Gopinath flux observer. Hence, the rotor flux, rotor speed and resistance have been estimated using only the electrical feedback magnitudes of the motor: the stator voltages and currents. The estimated rotor resistance is applied to the input of the flux observer and voltage compensator blocks. In order to evaluate the performances of the proposed method, a multiprocessor based simulation, using floating point DSP, has been performed.