gebhart/patrepeval huggingface dataset:
Evolutionary Algorithm-Guided Voxel-Encoding Printing of Functional Hard-Magnetic Soft Active Materials (2020)

Hard-magnetic soft active materials (hmSAMs) have attracted a great amount of research interests due to their fast-transforming, untethered control, as well as excellent programmability.However, the current direct-ink-write (DIW) printing-based fabrication of hmSAM parts and structures only permits programmable magnetic direction with a constant magnetic density.Also, the existing designs rely on the brute-force approach to generate the assignment of magnetization direction distribution, which can only produce intuitional deformations.These two factors greatly limit the design space and the application potentials of hmSAMs.In this work, we introduce a voxel-encoding DIW printing method to program both the magnetic density and direction distributions during the hmSAM printing.The voxel-encoding DIW printing is then integrated with an evolutionary algorithm (EA)-based design strategy to achieve the desired magnetic actuation and motion with complex geometry variations and curvature distributions.With the new EA-guided voxel-encoding DIW printing technique, we demonstrate the functional hmSAMs that produce complicated shape morphing with desired curvature distributions for advanced applications such as biomimetic motions.These demonstrations indicate that the proposed EA-guided voxel-encoding DIW printing method can significantly broaden the application potentials of the hmSAMs.
The Ameliorating Effect of Melatonin on Protamine Sulfate Induced Bladder Injury and its Relationship to Interstitial Cystitis (2003)

Increased malondialdehyde levels as a result of protamine sulfate induction lead us to propose that free radicals may have a critical role in this injury. The significant decrease in malondialdehyde levels in the protamine sulfate plus melatonin group was in accordance with morphological findings. Thus, melatonin appears to exert a urothelial protective activity in a bladder injury model.
Virtual autoencoder based recommendation system for individualizing head-related transfer functions (2013)

We propose a virtual autoencoder based recommendation system for learning a user's Head-related Transfer Functions (HRTFs) without subjecting a listener to impulse response or anthropometric measurements. Autoencoder neural-networks generalize principal component analysis (PCA) and learn non-linear feature spaces that supports both out-of-sample embedding and reconstruction; this may be applied to developing a more expressive low-dimensional HRTF representation. One application is to individualize HRTFs by tuning along the autoencoder feature spaces. We demonstrate this new approach by developing a virtual (black-box) user that can localize sound from query HRTFs reconstructed from those spaces. Standard optimization methods tune the autoencoder features based on the virtual user's feedback. Experiments with CIPIC HRTFs show that the virtual user can localize along out-of-sample directions and that optimization in the autoencoder feature space improves upon initial non-individualized HRTFs. Other applications of the representation are also discussed.
Coupled seismic and tracer test inversion for aquifer property characterization (1994)

Seismic and tracer test data can be combined to estimate the spatial patterns of aquifer properties. We present an algorithm that estimates the geometry of large‐scale lithologic zones, the effective hydraulic conductivities and seismic velocities for these zones, and the effective small‐scale dispersivity. The heart of this algorithm is our split inversion method, which extracts the geometry of lithologic zones from an estimated seismic velocity field. This method determines the zonation that best matches multiple types of data, such as seismic travel times and tracer concentrations. Although the current implementation of the algorithm uses only cross‐well seismic travel times and tracer concentrations, the algorithm could incorporate other data types that are sensitive to changes in large‐scale lithology. We demonstrate the approach for two synthetic sandy aquifers, one with interbedded clay lenses and another with interbedded silt and gravel lenses. These examples differ in the uniqueness of the relationship between seismic velocities and hydraulic conductivities. For these examples our algorithm successfully maps interwell heterogeneities and accurately estimates hydraulic and seismic parameters.
Protein tyrosine phosphorylation mediates TNF-induced endothelial-neutrophil adhesion in vitro (1998)

Proinflammatory cytokines initiate the vascular inflammatory response via the upregulation of adhesion molecules on the luminal endothelial surface. We investigated directly the role of protein tyrosine phosphorylation in the upregulation of the endothelial adhesion molecules, intercellular adhesion molecule 1 (ICAM-1) and E-selectin, and the consequent adhesion of neutrophils, after tumor necrosis factor (TNF)-alpha-stimulation of human aortic endothelial cells in vitro. Time- and dose-dependent TNF-alpha-stimulated ICAM-1 and E-selectin upregulation and neutrophil adhesion each were suppressed by tyrosine kinase inhibitors, including genistein (200 microM), but not genistein, its isoflavone analog without tyrosine kinase inhibitory activity. Tyrphostin AG 126, a synthetic selective tyrosine kinase inhibitor, also suppressed ICAM-1 and E-selectin upregulation and neutrophil adhesion, each in a dose-dependent manner, whereas tyrphostin AG 1288 had no effect. Tyrosine phosphorylation of two proteins (85 and 145 kDa in the cytoskeleton fraction) found minutes after TNF-alpha-stimulation was also inhibited by genistein. These findings suggest that, in endothelial cells, TNF-alpha upregulates ICAM-1 and E-selectin expression and consequent neutrophil adhesion via protein tyrosine phosphorylation.
Time-Dependent Increase in Network Response to Stimulation (2015)

In vitro neuronal cultures have become a popular method with which to probe network-level neuronal dynamics and phenomena in controlled laboratory settings. One of the key dynamics of interest in these in vitro studies has been the extent to which cultured networks display properties indicative of learning. Here we demonstrate the effects of a high frequency electrical stimulation signal in training cultured networks of cortical neurons. Networks receiving this training signal displayed a time-dependent increase in the response to a low frequency probing stimulation, particularly in the time window of 20-50 ms after stimulation. This increase was found to be statistically significant as compared to control networks that did not receive training. The timing of this increase suggests potentiation of synaptic mechanisms. To further investigate this possibility, we leveraged the powerful Cox statistical connectivity method as previously investigated by our group. This method was used to identify and track changes in network connectivity strength.
A fully integrated high-performance FM stereo decoder (1992)

A custom FM stereo decoder IC which uses switched-capacitor and linear filters, linear MOS and bipolar circuitry, and digital MOS circuitry to eliminate the requirements for accurate external components and external adjustments is described. The IC includes a combined analog/digital VCO and PLL, a switched-capacitor sine-wave multiplier, and a switched-capacitor pilot detector and cancellation stage. An output S/N ratio greater than 80 dB and output distortion less than 0.1% have been achieved. The part is 26000 mils/sup 2/ and is implemented in a 3 mu m n-well CMOS process containing poly-to-poly capacitors, n-p-n's, and ion-implanted poly resistors.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Who Meets the Standrads: A Multidimensional Approach (2011)

We consider here the evaluation of the performance of a society with respect to a given set of targets. We provide a characterization of an intuitive evaluation formula that consists of the mean of the shares of the achievements in the targets. The criterion so obtained permits one not only to endogenously determine who meets the standards and who does not, but also to quantify the degree of fulfilment. Two empirical illustrations are provided: the compliance of the European Union Stability and Growth Pact, on the one hand, and the evaluation of research excellence in the Spanish universities, on the other hand.
A scalable high-speed current-mode winner-take-all network for VLSI neural applications (1995)

This paper describes a very flexible, high-speed current-mode winner-take-all network (WTA) for use in artificial neural systems. Since the WTA is based on a network of identical current switching cells requiring only adjacent transistor matching, it is particularly suited to use in large systems. In addition, since the network is based on a tree structure, very little space is occupied by interconnect, thus ensuring a very compact layout. This WTA has current (matching score) inputs and provides a buffered, binary encoded voltage output.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Three-Hour Molecular Detection of <i>Campylobacter</i> , <i>Salmonella</i> , <i>Yersinia</i> , and <i>Shigella</i> Species in Feces with Accuracy as High as That of Culture (2010)

Campylobacter jejuni and Salmonella, Shigella, and Yersinia species (along with Shiga toxin-producing Escherichia coli) are the most common causes of acute bacterial diarrheal disease in the United States. Current detection techniques are time-consuming, limiting usefulness for patient care. We developed and validated a panel of rapid PCR assays for the detection and identification of C. jejuni, C. coli, Salmonella, and Yersinia species and Shigella and enteroinvasive E. coli in stool samples. A total of 392 archived stool specimens, previously cultured for enteric pathogens, were evaluated by PCR. Overall, 104 stool specimens had been culture positive (C. jejuni/coli [n = 51], Salmonella species [n = 42], Shigella species [n = 6], and Yersinia species [n = 5]). Compared to culture, the overall sensitivity and specificity of PCR detection of these organisms were 92 and 98% (96/104 and 283/288), respectively, from fresh or Cary Blair stool (P = 0.41); 87 and 98% (41/47 and 242/246), respectively, from fresh stool (P = 0.53); and 96 and 98% (55/57 and 41/42), respectively, from Cary Blair stool (P = 0.56). For individual genera, PCR was as sensitive as the culture method, with the exception of Salmonella culture using selenite enrichment for which PCR was less sensitive than culture from fresh, but not Cary Blair (P = 0.03 and 1.00, respectively) stools. This PCR assay panel for the rapid diagnosis of acute infectious bacterial diarrheal pathogens has a sensitivity and specificity equivalent to that of culture for stools in Cary Blair transport medium. Paired with reflexive culture of stools testing positive, this should provide an improvement in care for patients with acute infectious diarrheal disease.
The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene. (1994)

The lymphocyte-specific DNA-binding protein LyF-1 interacts with a critical control element in the terminal deoxynucleotidyltransferase (TdT) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. We have purified LyF-1 and have obtained a partial amino acid sequence from proteolytic peptides. The amino acid sequence suggests that LyF-1 is a zinc finger protein encoded by the Ikaros gene, which previously was implicated in T-cell development. Recombinant Ikaros expressed in Escherichia coli bound to the TdT promoter, and antisera directed against the recombinant protein specifically blocked the DNA-binding activity of LyF-1 in crude extracts. Further analysis revealed that at least six distinct mRNAs are derived from the Ikaros/LyF-1 gene by alternative splicing. Only two of the isoforms possess the N-terminal zinc finger domain that is necessary and sufficient for TdT promoter binding. Although both of these isoforms bound to similar sequences in the TdT, lambda 5, VpreB, and lck promoters, one isoform contains an additional zinc finger that resulted in altered recognition of some binding sites. At least four of the Ikaros/LyF-1 isoforms were detectable in extracts from B- and T-cell lines, with the relative amounts of the isoforms varying considerably. These data reveal that the LyF-1 protein is encoded by specific mRNAs derived from the alternatively-spliced Ikaros gene, suggesting that this gene may be important for the early stages of both B- and T-lymphocyte development.
História, imaginário e mentalidades: delineamentos possíveis (2007)

Este artigo – voltado para as discussoes estabelecidas desde a segunda metade do seculo XX entre as possiveis interacoes entre Historia, cultura e imaginario – busca desenvolver um paralelo comparativo entre historia das mentalidades, Psicoistoria e historia do imaginario, esclarecendo alguns aspectos relacionados a essas modalidades da Historia e discutindo a historiografia pertinente a cada um desses campos. O artigo remete a obra recentemente publicada pelo autor deste texto, cujo principal objetivo e o de elaborar uma visao panorâmica das diversas modalidades da Historia nos dias de hoje.
RADIOIMMUNOASSAY OF OESTRONE AND OESTRADIOL IN HUMAN PLASMA (1972)

ABSTRACT A simple method for the determination of oestrone and oestradiol in human plasma is described and evaluated in terms of theoretical and practical errors. The oestrogens are separated on columns of Sephadex LH 20 and, after equilibration with antiserum, the unbound steroid is removed with dextran-coated charcoal. The mean total random theoretical error is calculated to be 22 %; the coefficients of variation of replicate analyses on pooled female plasma were from 13–18 % for both steroids; in male plasma the values were 17 % for oestradiol and 27 % for oestrone. The concentrations of oestradiol, expressed in pg/ml ± sd) in samples collected from healthy women during the menstrual cycle were 69 ± 56 (days 1–10); 126 ± 66 (days 11–20) and 99 ± 54 (days 21–30). Corresponding values for oestrone were 119 ± 46, 156 ± 41 and 156 ± 27.
Adsorption of Polycyclic Aromatic Hydrocarbons by Graphene and Graphene Oxide Nanosheets (2014)

The adsorption of naphthalene, phenanthrene, and pyrene onto graphene (GNS) and graphene oxide (GO) nanosheets was investigated to probe the potential adsorptive sites and molecular mechanisms. The microstructure and morphology of GNS and GO were characterized by elemental analysis, XPS, FTIR, Raman, SEM, and TEM. Graphene displayed high affinity to the polycyclic aromatic hydrocarbons (PAHs), whereas GO adsorption was significantly reduced after oxygen-containing groups were attached to GNS surfaces. An unexpected peak was found in the curve of adsorption coefficients (Kd) with the PAH equilibrium concentrations. The hydrophobic properties and molecular sizes of the PAHs affected the adsorption of G and GO. The high affinities of the PAHs to GNS are dominated by π-π interactions to the flat surface and the sieving effect of the powerful groove regions formed by wrinkles on GNS surfaces. In contrast, the adsorptive sites of GO changed to the carboxyl groups attaching to the edges of GO because the groove regions disappeared and the polar nanosheet surfaces limited the π-π interactions. The TEM and SEM images initially revealed that after loading with PAH, the conformation and aggregation of GNS and GO nanosheets dramatically changed, which explained the observations that the potential adsorption sites of GNS and GO were unusually altered during the adsorption process.
Is the Perceived Association between <i>Chlamydia pneumoniae</i> and Vascular Diseases Biased by Methodology? (2004)

Inter- and intralaboratory inconsistencies in detection rates of Chlamydia pneumoniae in vascular specimens have been demonstrated. In this study, 66 vascular tissue specimens from 66 patients with vascular disease were tested by three PCR assays: a 16S PCR-based reverse line blot (RLB) assay, a single-step PCR, and a nested PCR. Also, we explored the impacts of different DNA polymerase enzymes on the results based on gel electrophoresis and hybridization. The PCR results by gel electrophoresis in the single-step PCR depended on which DNA polymerase was used. All samples were negative with AmpliTaq Gold DNA polymerase, and 54.5% (36 of 66) were positive with the conventional Taq DNA polymerase. All samples were negative after hybridization with a C. pneumoniae-specific probe. In the nested PCR, all specimens were negative by gel electrophoresis and after hybridization. The RLB assay failed to detect C. pneumoniae in any specimen; however, 20 specimens were Chlamydia sp. positive. The sequence analysis of six of these samples demonstrated Chlamydia-like organisms. RLB detected Chlamydia sp. DNA in water and in the elution buffer after passage of the Qiagen columns (11 of 40). This study identified factors that may influence the detection of C. pneumoniae DNA in vascular tissues and consequently bias the perception of a link between C. pneumoniae and vascular diseases. The following are strongly recommended: to use DNA polymerases that have to be activated, to decontaminate with dUTP-uracil-DNA glycosylase, to hybridize with specific probes, to include sufficient controls, and to use molecular grade water.
Antimicrobial activity of amoxicillin and other penicillins against clinical isolates from bovine udders. (1985)

Amoxicillin, a semisynthetic penicillin, showed a strong antibacterial activity against S.aureus (64 strains), coagulase-negative staphylococci (93 strains), E.coli (61 strains), corynebacteria and streptococci isolated from bovine mastitis
Explicación de Clarice (2007)

El artículo se ocupa de las ideas de Clarice Lispector en sus últimos libros de la década del 70, época de su carrera literaria conocida, según sugestión de la propia escritora, como “la hora de la basura”. El texto postula que, en oposición a los narradores latinoamericanos del boom, su experiencia de la crisis de la cultura occidental la conduce antes en dirección de la “imaginación del desastre” y la “imaginación pop” que en dirección de la “imaginación humanista” o la “imaginación dialéctica”.
Gas‐permeable membranes and co‐culture with fibroblasts enable high‐density hepatocyte culture as multilayered liver tissues (2011)

To engineer reliable in vitro liver tissue equivalents expressing differentiated hepatic functions at a high level and over a long period of time, it appears necessary to have liver cells organized into a three-dimensional (3D) multicellular structure closely resembling in vivo liver cytoarchitecture and promoting both homotypic and heterotypic cell-cell contacts. In addition, such high density 3D hepatocyte cultures should be adequately supplied with nutrients and particularly with oxygen since it is one of the most limiting nutrients in hepatocyte cultures. Here we propose a novel but simple hepatocyte culture system in a microplate-based format, enabling high density hepatocyte culture as a stable 3D-multilayer. Multilayered co-cultures of hepatocytes and 3T3 fibroblasts were engineered on collagen-conjugated thin polydimethylsiloxane (PDMS) membranes which were assembled on bottomless frames to enable oxygen diffusion through the membrane. To achieve high density multilayered co-cultures, primary rat hepatocytes were seeded in large excess what was rendered possible due to the removal of oxygen shortage generally encountered in microplate-based hepatocyte cultures. Hepatocyte/3T3 fibroblasts multilayered co-cultures were maintained for at least 1 week; the so-cultured cells were normoxic and sustained differentiated metabolic functions like albumin and urea synthesis at higher levels than hepatocytes monocultures. Such a microplate-based cell culture system appears suitable for engineering in vitro miniature liver tissues for implantation, bioartificial liver (BAL) development, or chemical/drug screening.
Effect of 2-Month Controlled Green Tea Intervention on Lipoprotein Cholesterol, Glucose, and Hormone Levels in Healthy Postmenopausal Women (2012)

There have been no controlled intervention studies to investigate the effects of green tea on circulating hormone levels, an established breast cancer risk factor. We conducted a double-blind, randomized, placebo-controlled intervention study to investigate the effect of the main green tea catechin, epigallocatechin gallate (EGCG), taken in a green tea extract, polyphenon E (PPE). Postmenopausal women (n = 103) were randomized into three arms: placebo, 400-mg EGCG as PPE, or 800-mg EGCG as PPE as capsules per day for 2 months. Urinary tea catechin and serum estrogen, androgen, lipid, glucose-related markers, adiponectin, and growth factor levels were measured at baseline and at the end of months 1 and 2 of intervention. On the basis of urinary tea catechin concentrations, compliance was excellent. Supplementation with PPE did not produce consistent patterns of changes in estradiol (E2), estrone (E1), or testosterone (T) levels. Low-density lipoprotein (LDL)-cholesterol decreased significantly in both PPE groups but was unchanged in the placebo group; the change in LDL-cholesterol differed between the placebo and PPE groups (P = 0.02). Glucose and insulin levels decreased nonsignificantly in the PPE groups but increased in the placebo group; statistically significant differences in changes in glucose (P = 0.008) and insulin (P = 0.01) were found. In summary, green tea (400- and 800-mg EGCG as PPE; ∼5-10 cups) supplementation for 2 months had suggestive beneficial effects on LDL-cholesterol concentrations and glucose-related markers.
Resolution measurement for ion mobility spectrometry (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTResolution measurement for ion mobility spectrometrySouji. Rokushika, Hiroyuki. Hatano, Michael A. Baim, and Herbert H. HillCite this: Anal. Chem. 1985, 57, 9, 1902–1907Publication Date (Print):August 1, 1985Publication History Published online1 May 2002Published inissue 1 August 1985https://pubs.acs.org/doi/10.1021/ac00286a023https://doi.org/10.1021/ac00286a023research-articleACS PublicationsRequest reuse permissionsArticle Views1019Altmetric-Citations108LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts