gebhart/patrepeval huggingface dataset:
Identification and Functional Characterization of Adipose-specific Phospholipase A2 (AdPLA) (2008)

Phospholipases A(2) (PLA(2)s) catalyze hydrolysis of fatty acids from the sn-2 position of phospholipids. Here we report the identification and characterization of a membrane-associated intracellular calcium-dependent, adipose-specific PLA(2) that we named AdPLA (adipose-specific phospholipase A(2)). We found that AdPLA was highly expressed specifically in white adipose tissue and was induced during preadipocyte differentiation into adipocytes. Clearance of AdPLA by immunoprecipitation significantly decreased PLA activity in white adipose tissue lysates but had no effect on liver lysates, where expression was hardly detectable. In characterizing AdPLA, we employed radiochemical assays with TLC analysis of the enzyme activity of lysates from COS-7 cells overexpressing AdPLA. For kinetic studies, we produced purified recombinant AdPLA for use in a lipoxidase-coupled spectrophotometric assay. AdPLA generated free fatty acid and lysophospholipid from phosphatidylcholine with a preference for hydrolysis at the sn-2 position. Although we found low but detectable lysophospholipase activity, AdPLA showed no significant activity against a variety of other lipid substrates. Calcium was found to activate AdPLA but was not essential for activity. Studies with known phospholipase inhibitors, including bromoenolactone, methyl arachidonyl fluorophosphate, AACOCF(3), 7,7-dimethyl-5,8-eicosadienoic acid, and thioetheramide, supported that AdPLA is a phospholipase. Mutational studies showed that His-23 and Cys-113 are critical for activity of AdPLA and suggested that AdPLA is likely a His/Cys PLA(2). Overall, although AdPLA is similar to other histidine phospholipases in pH and calcium dependence, AdPLA showed different characteristics in many regards, including predicted catalytic mechanism. AdPLA may therefore represent the first member of a new group of PLA(2)s, group XVI.
Supporting Asymmetric Traffic in a TDD/CDMA Cellular Network via Interference-Aware Dynamic Channel Allocation and Space–Time LMMSE Joint Detection (2009)

In a time-division duplexing (TDD)/code-division multiple-access (CDMA) cellular network with asymmetric data traffic, dynamic channel allocation (DCA) enhances resource utilization compared with fixed channel allocation (FCA). However, it also induces base-to-base and mobile-to-mobile crossed-slot intercell interference, which can severely degrade system performance. To deal with this problem, a decentralized scheme is proposed, which combines an interference-aware DCA algorithm with space-time linear minimum-mean-square-error (LMMSE) joint detection at the base and mobile stations. The former assigns active links to timeslots in a way that crossed-slot interference is mitigated, while the latter suppresses the remaining intercell interference (along with intersymbol and intracell interference), exploiting its spatio-temporal autocorrelation statistics. The performance of this scheme is evaluated in terms of downlink and uplink signal-to-interference-plus-noise ratio (SINR) outage and average throughput via analytical approximations and Monte Carlo simulations, and it is compared with that of benchmark random DCA (RDCA) and FCA schemes. The cases of single- and dual-antenna reception with perfect and imperfect channel state information are examined. It is shown that the proposed scheme achieves higher average throughput than FCA (particularly for dual-antenna reception) as well as RDCA (for heavy traffic loads). These throughput gains are more significant in uplink than in downlink.
Application of HaloTag Protein to Covalent Immobilization of Recombinant Proteins for Single Molecule Force Spectroscopy (2010)

We have developed the HaloTag system for the covalent immobilization of polyproteins onto a mica substrate for single molecule force spectroscopy using the atomic force microscope. A recombinant fusion polyprotein of titin I27 with HaloTag7 protein was produced, and the covalent and site-specific attachment on a HaloTag-ligand-modified mica surface was confirmed by force-extension measurements. Two mechanical unfolding intermediates of HaloTag7 protein were found by contour length analysis. This tethering method allows site-specific covalent immobilization of a protein that complements the standard method utilizing thiol-gold interaction, thus facilitating force-extension measurements for cysteine-containing proteins.
Lysosome signaling controls the migration of dendritic cells (2017)

Dendritic cells (DCs) patrol their environment by linking antigen acquisition by macropinocytosis to cell locomotion. DC activation upon bacterial sensing inhibits macropinocytosis and increases DC migration, thus promoting the arrival of DCs to lymph nodes for antigen presentation to T cells. The signaling events that trigger such changes are not fully understood. We show that lysosome signaling plays a critical role in this process. Upon bacterial sensing, lysosomal calcium is released by the ionic channel TRPML1 (transient receptor potential cation channel, mucolipin subfamily, member 1), which activates the actin-based motor protein myosin II at the cell rear, promoting fast and directional migration. Lysosomal calcium further induces the activation of the transcription factor EB (TFEB), which translocates to the nucleus to maintain TRPML1 expression. We found that the TRPML1-TFEB axis results from the down-regulation of macropinocytosis after bacterial sensing by DCs. Lysosomal signaling therefore emerges as a hitherto unexpected link between macropinocytosis, actomyosin cytoskeleton organization, and DC migration.
Brief history of agricultural systems modeling (2016)

Agricultural systems science generates knowledge that allows researchers to consider complex problems or take informed agricultural decisions. The rich history of this science exemplifies the diversity of systems and scales over which they operate and have been studied. Modeling, an essential tool in agricultural systems science, has been accomplished by scientists from a wide range of disciplines, who have contributed concepts and tools over more than six decades. As agricultural scientists now consider the "next generation" models, data, and knowledge products needed to meet the increasingly complex systems problems faced by society, it is important to take stock of this history and its lessons to ensure that we avoid re-invention and strive to consider all dimensions of associated challenges. To this end, we summarize here the history of agricultural systems modeling and identify lessons learned that can help guide the design and development of next generation of agricultural system tools and methods. A number of past events combined with overall technological progress in other fields have strongly contributed to the evolution of agricultural system modeling, including development of process-based bio-physical models of crops and livestock, statistical models based on historical observations, and economic optimization and simulation models at household and regional to global scales. Characteristics of agricultural systems models have varied widely depending on the systems involved, their scales, and the wide range of purposes that motivated their development and use by researchers in different disciplines. Recent trends in broader collaboration across institutions, across disciplines, and between the public and private sectors suggest that the stage is set for the major advances in agricultural systems science that are needed for the next generation of models, databases, knowledge products and decision support systems. The lessons from history should be considered to help avoid roadblocks and pitfalls as the community develops this next generation of agricultural systems models.
Sp1 Regulates Chromatin Looping between an Intronic Enhancer and Distal Promoter of the Human Heme Oxygenase-1 Gene in Renal Cells (2010)

HO-1 (heme oxygenase-1) is an inducible microsomal enzyme that catalyzes the degradation of pro-oxidant heme. The goal of this study was to characterize a minimal enhancer region within the human HO-1 gene and delineate its role in modulating HO-1 expression by participation with its promoter elements in renal epithelial cells. Deletion analysis and site-directed mutagenesis identified a 220-bp minimal enhancer in intron 1 of the HO-1 gene, which regulates hemin-mediated HO-1 gene expression. Small interfering RNA, decoy oligonucleotides, site-directed mutagenesis, and chromatin immunoprecipitation assays confirmed the functional interaction of Sp1 with a consensus binding sequence within the 220-bp region. Mutations of regulatory elements within the -4.5 kb promoter region (a cyclic AMP response and a downstream NF-E2/AP-1 element, both located at -4.0 kb, and/or an E-box sequence located at -44 bp) resulted in the loss of enhancer activity. A chromosome conformation capture assay performed in human renal epithelial (HK-2) cells demonstrated hemin-inducible chromatin looping between the intronic enhancer and the -4.0 kb promoter region in a time-dependent manner. Restriction digestion with ApaLI (which cleaves the 220-bp enhancer) led to a loss of stimulus-dependent chromatin looping. Sp1 small interfering RNA and mithramycin A, a Sp1 binding site inhibitor, resulted in loss of the loop formation between the intronic enhancer and the distal HO-1 promoter by the chromosome conformation capture assay. These results provide novel insight into the complex molecular interactions that underlie human HO-1 regulation in renal epithelial cells.
Monorail Maglev Technology (1991)

This paper summarizes work presently under way at MIT to determine some of the data needed to establish a national U. S. maglev system. Our work is focused on developing a narrow beam guideway for Electro Dynamic Suspension and Linear Synchronous Motor propulsion; a design that meets the needs of the U. S now and in the future. This guideway and motor are adaptable to a range of vehicle sizes and speeds so as to allow appropriate evolution. Present demand estimates are typically low enough that guideway cost is significantly higher than the sum of vehicle, labor, and maintenance costs. However, we know that with time the capital cost will be paid off and demand will increase until these other costs dominate. Thus it is necessary to be very careful in creating a guideway and motor design that will be suitable for the U. S. for many decades.
A four-layer model for security of digital rights management (2008)

Defining Digital Rights Management (DRM) is a complex task. There is no unique universal definition. There are many legal, economic, functional, and technical definitions. This complexity induces also that there is not one unique modeling of DRM. Each model should help to compare different DRM systems and easily highlight the differences and the similarities between them. One of the weaknesses of the current models is that none puts specifically the focus on the most important characteristics of DRM: protection of content and rights management. We propose a four-layer model that complements traditional ones. Using trust layer, rights management layer, rights enforcement layer, and content protection layer, this model is security oriented. It is suitable to describe any content protections such as DRM, conditional access, copy protection or even pre-recorded content protection systems.
Studies with the Amine Adducts of β-Benzoylacrylic Acid and its Methyl Ester<sup>1</sup> (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies with the Amine Adducts of β-Benzoylacrylic Acid and its Methyl Ester1Norman H. Cromwell, Paul L. Creger, and Kenneth E. CookCite this: J. Am. Chem. Soc. 1956, 78, 17, 4412–4416Publication Date (Print):September 1, 1956Publication History Published online1 May 2002Published inissue 1 September 1956https://pubs.acs.org/doi/10.1021/ja01598a058https://doi.org/10.1021/ja01598a058research-articleACS PublicationsRequest reuse permissionsArticle Views157Altmetric-Citations28LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Extraesophageal pediatric reflux: 24-hour double-probe pH monitoring of 222 children. (1997)

Although extraesophageal gastric reflux has been implicated as a cause of many pediatric airway and respiratory diseases, its prevalence in these conditions remains unknown due to the relative lack of sensitivity and/or specificity of traditional reflux testing methods. A prospective study of 222 children (ages 1 day to 16 years) was performed with 24-hour double-probe (simultaneous esophageal and pharyngeal) pH monitoring. Seventy-six percent (168/222) of the study population had abnormal findings in either one or both of the pH probes. Of those, 46% (78/168) had pharyngeal reflux (extraesophageal gastric acid documented by the pharyngeal probe), despite having normal esophageal acid exposure times according to the esophageal probe. Thus, had the pharyngeal probe not been used, 46% of the children with documented extraesophageal (pharyngeal) reflux would have been falsely presumed to have normal reflux parameters. Patients with laryngeal abnormalities, pulmonary abnormalities, and emesis had significantly more pharyngeal acid reflux (p < .001) than patients with nonrespiratory symptoms. These data suggest that extraesophageal reflux may be underestimated by single-probe intraesophageal monitoring alone, and that laryngopharyngeal reflux may play a role in the pathogenesis of the conditions studied.
Base para la planificación industrial de Bahía Blanca (1952)

Base para la planificación industrial de Bahía Blanca
Which to View (2016)

Email is one of the most important communication tools today, but email overload resulting from the large number of unimportant or irrelevant emails is causing trillion-level economy loss every year. Thus personalized email prioritization algorithms are of urgent need. Despite lots of previous effort on this topic, broadcast email, an important type of email, is overlooked in previous literature. Broadcast emails are significantly different from normal emails, introducing both new challenges and opportunities. On one hand, lack of real senders and limited user interactions invalidate the key features exploited by traditional email prioritization algorithms; on the other hand, thousands of receivers for one broadcast email bring us the opportunity to predict importance through collaborative filtering. However, broadcast emails face a severe cold-start problem which hinders the direct application of collaborative filtering. In this paper, we propose the first framework for broadcast email prioritization by designing a novel active learning model that considers the collaborative filtering, implicit feedback and time sensitive responsiveness features of broadcast emails. Our method is thoroughly evaluated on a large scale real world industrial dataset from Samsung Electronics. Our method is proved highly effective and outperforms state-of-the-art personalized email prioritization methods.
KDA: a knowledge-based database assistant with a query guiding facility (1992)

A knowledge-based database assistant (KDA) which integrates a natural language query system with a skeleton-based query guiding facility is provided. When a user works with the KDA natural language query system, the query guiding facility can supply several kinds of skeletons to guide users in performing database retrieval tasks. A semantic network model, S-Net, is introduced to represent the knowledge for natural language query processing and skeleton generation. Methods for implementing the system are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Stable Si–H groups on silica surfaces (1968)

C. Morterra and M. J. D. Low, Chem. Commun. (London), 1968, 203 DOI: 10.1039/C19680000203
Epitaxial growth of Si deposited on (100) Si (1980)

Epitaxial growth of deposited amorphous Si on chemically cleaned (100) Si has been found and layer-by-layer growth occurred at rates comparable to those in self-ion-implanted-amorphous Si. There is no evidence for appreciable oxygen penetration into the deposited layer during storage in air. The critical factors in achieving epitaxial growth are fast (∼50 Å/sec) deposition of Si onto a surface cleaned with a HF dip as a last rinse before loading into the vacuum system. Channeling and transmission electron microscopy measurements indicated that the epitaxial layers are essentially defect free. Secondary-ion mass spectroscopic analysis showed about 1014 oxygen/cm2 at the amorphous/crystal interface. With either higher interfacial oxygen coverage or slow (∼2 Å/sec) deposition, epitaxial growth rates are significantly slower.
PICO extraction by combining the robustness of machine-learning methods with the rule-based methods (2015)

Machine-learning methods (MLMs) are robust methods in the extraction of the information; they have been also used in the extraction of PICO elements in order to answer clinical questions; MLMs are only used at coarse-grained level in PICO extraction, because of lack of training corpora for PICO at the fine-grained level. Coarse-grained level cannot explore the semantics within the sentence for use as a means of relevance between different answers. We propose a hybrid approach combining the robustness of MLMs and the fine grained level of RBMs to enhance PICO extraction process and facilitate the validity and the pertinence of the answers to clinic questions formulated with the PICO framework.
Infrared Laser Generation Of Heterogeneous Catalysts And Laser-Induced Reactions At Catalytic Surfaces (1984)

It has been demonstrated that continuous wave infrared CO<sub>2</sub> laser radiation can be util-ized to rapidly produce active catalysts from inert precursors. The activity and selectiv-ity of Ca0 produced from Ca(OH)<sub>2</sub> for the isomerization of 1-butene to cis- and trans-2-but-ene is discussed. Variation of the laser irradiation time produces catalytic activity and selectivity qualitatively similar to that resulting from conventional calcination at different temperatures. Pulsed infrared laser-induced reactions at catalytic surfaces are also discussed with emphasis on the dehydrobromination of 2-bromopropane and ethylene elimination from glycine ethyl ester hydrochloride at BaSO<sub>4</sub> , A1PO<sub>4</sub>, and similar surfaces. Correlations are made of the extent of reaction with various experimental parameters including nature of the catalyst, laser frequency, laser fluence, number of laser pulses, and reagent-catalyst ratio.
Studies on 2',3'-dideoxy-2',3'-didehydropyrimidine nucleosides. II. N4-benzoyl-2',3'-dideoxy-2',3'-didehydrocytidine as a prodrug of 2',3'-dideoxy-2',3'-didehydrocytidine(DDCN). (1989)

N4-Benzoyl-2',3'-dideoxy-2',3'-didehydrocytidine (Bz-DDCN) was synthesized as a novel prodrug of 2',3'-dideoxy-2',3'-didehydrocytidine (DDCN), which is a reverse transcriptase inhibitor and is considered to be a potential anti-acquired immunodeficiency syndrome agent. Chemical and enzymatic regeneration of DDCN from the prodrug has been investigated in both in vitro and in vivo experiments. Bz-DDCN regenerated DDCN under basic conditions (greater than pH 8), while cleavage of the N-glycosidic linkage and production of N4-benzoylcytosine were observed under acidic conditions (less than pH 6). DDCN was enzymatically regenerated from the prodrug in the presence of several enzyme preparations, including human plasma. DDCN and Bz-DDCN were intravenously administered to mice and the plasma concentrations of DDCN and the prodrug were measured. Though DDCN levels following direct DDCN administration decreased exponentially with a half-life of 14.5 min, the plasma levels of DDCN following the prodrug administration were sustained above 2 microM for over 3 h.
Antibacterial effects of biologically active ingredients in hop provide promising options to fight infections by pathogens including multi-drug resistant bacteria (2022)

Antibiotic resistance constitutes a global threat to the health care systems. The number of infections due to multidrug-resistant (MDR) bacteria increases progressively resulting in an estimated annual number of 750,000 fatal cases worldwide. Additionally, the lack of novel antibiotic compounds worsens the dilemma. Hence, there is an urgent need for alternative ways to fight antibiotic resistance. One option may be natural compounds with antibacterial properties such as hop and its biologically active ingredients which are used in traditional medicine since ancient times. This prompted us to perform an actual literature survey regarding the antibacterial properties of biologically active ingredients in hop including humulone, lupulone and xanthohumol. The 20 included studies revealed that lupulone and xanthohumol do in fact inhibit the growth of Gram-positive bacteria in vitro. In combination with distinct antibiotic compounds the hop ingredients can even exert synergistic effects resulting in enhanced antibiotic activities against defined Gram-positive and Gram-negative bacteria. In conclusion, biologically active ingredients in hop including lupulone and xanthohumol may be potential antibiotic compounds which either alone or in combination with other antibacterial substances open novel avenues in the combat of infections caused by pathogenic including MDR bacteria.
Sensitivity and stability of a radiation-balanced laser system (2002)

It has been previously shown that it is theoretically possible to build a solid-state laser that generates no internal heat. This is accomplished through a detailed balance of the stimulated and spontaneous emission. Such a device is called a radiation-balanced laser. Here, we analyze laser operation when conditions deviate from perfect balance. Sensitivity and stability analyses are presented for perturbations in the field parameters and temperature. We show that the radiation balance is a stable equilibrium under both transient and steady-state perturbations. Limits are derived on the magnitude of allowable spatial perturbations, and techniques for spatial mode matching are discussed. Numerical simulations of 1-/spl mu/m laser operation of Yb-doped potassium gadolinium tungstate are provided as an example.
赋予水刺材料以装饰性→增值＋高效 (2005)

对水刺非织造材料厂商而言,在试图为全球市场提供服务的时候,正面临着在世界范围普遍存在的问题,即高企的原材料成本,尤其是基于石油供应的纤维原材料的成本,以及供需方之间的货运费用.为使购买者愿意消化吸收因远距离的运输而产生的成本,那么提高产品的价值和收益就显得非常必要.为此,赋予水刺材料一定的装饰性就是很好的选择.