gebhart/patrepeval huggingface dataset:
A systematic approach to the extraction of diphone elements from natural speech (1986)

Synthetic speech can be generated with an unrestricted vocabulary by concatenating stored units such as diphone elements. When joining speech segments that were not adjacent in the original context they were taken from, discontinuities in the spectral envelope may arise that impair intelligibility. The method proposed here attempts to find optimum diphone boundaries in order to minimize these discontinuities, Steady-state zones of all phones carrying a diphone boundary are specified by means of a centroid vector. Based on the centroids and on an objective distance measure, hypothetical boundary cost functions are defined. Their minimization together with the evaluation of a set of additional rules determines the boundary locations. A rhyme test carried out with speech generated by concatenating diphone elements extracted according to this method yielded an intelligibility score of 96.7 percent for isolated words.
Synthetic mRNA nanoparticle-mediated restoration of p53 tumor suppressor sensitizes <i>p53</i> -deficient cancers to mTOR inhibition (2019)

Loss of function in tumor suppressor genes is commonly associated with the onset/progression of cancer and treatment resistance. The <i>p53</i> tumor suppressor gene, a master regulator of diverse cellular pathways, is frequently altered in various cancers, for example, in ~36% of hepatocellular carcinomas (HCCs) and ~68% of non-small cell lung cancers (NSCLCs). Current methods for restoration of p53 expression, including small molecules and DNA therapies, have yielded progressive success, but each has formidable drawbacks. Here, a redox-responsive nanoparticle (NP) platform is engineered for effective delivery of <i>p53</i>-encoding synthetic messenger RNA (mRNA). We demonstrate that the synthetic <i>p53</i>-mRNA NPs markedly delay the growth of <i>p53</i>-null HCC and NSCLC cells by inducing cell cycle arrest and apoptosis. We also reveal that p53 restoration markedly improves the sensitivity of these tumor cells to everolimus, a mammalian target of rapamycin (mTOR) inhibitor that failed to show clinical benefits in advanced HCC and NSCLC. Moreover, cotargeting of tumor-suppressing p53 and tumorigenic mTOR signaling pathways results in marked antitumor effects in vitro and in multiple animal models of HCC and NSCLC. Our findings indicate that restoration of tumor suppressors by the synthetic mRNA NP delivery strategy could be combined together with other therapies for potent combinatorial cancer treatment.
Pregnenolone-16α-carbonitrile inhibits rodent liver fibrogenesis via PXR (pregnane X receptor)-dependent and PXR-independent mechanisms (2005)

The effect of liver growth stimulation [using the rodent PXR (pregnane X receptor) activator PCN (pregnenolone-16alpha-carbonitrile)] in rats chronically treated with carbon tetrachloride to cause repeated hepatocyte necrosis and liver fibrogenesis was examined. PCN did not inhibit the hepatotoxicity of carbon tetrachloride. However, transdifferentiation of hepatic stellate cells and the extent of fibrosis caused by carbon tetrachloride treatment was significantly inhibited by PCN in vivo. In vitro, PCN directly inhibited hepatic stellate cell transdifferentiation to a profibrogenic phenotype, although the cells did not express the PXR (in contrast with hepatocytes), suggesting that PCN acts independently of the PXR. Mice with a functionally disrupted PXR gene (PXR-/-) did not respond to the antifibrogenic effects of PCN, in contrast with wild-type (PXR+/+) mice, demonstrating an antifibrogenic role for the PXR in vivo. However, PCN inhibited the transdifferentiation of PXR-/--derived mouse hepatic stellate cells in vitro, confirming that there is also a PXR-independent antifibrogenic effect of PCN through a direct interaction with hepatic stellate cells. These data suggest that the PXR is antifibrogenic in rodents in vivo and that a PXR-independent target for PXR activators exists in hepatic stellate cells that also functions to inhibit fibrosis.
Mobile Phone-Based Field Monitoring for Satsuma Mandarin and Its Application to Watering Advice System (2012)

This paper describes a mobile phone-based data logging system for monitoring the growing status of Satsuma mandarin, a type of citrus fruit, in the field. The system can provide various feedback to the farm producers with collected data, such as visualization of related data as a timeline chart or advice on the necessity of watering crops. It is important to collect information on environment conditions, plant status and product quality, to analyze it and to provide it as feedback to the farm producers to aid their operations. This paper proposes a novel framework of field monitoring and feedback for open-field farming. For field monitoring, it combines a low-cost plant status monitoring method using a simple apparatus and a Field Server for environment condition monitoring. Each field worker has a simple apparatus to measure fruit firmness and records data with a mobile phone. The logged data are stored in the database of the system on the server. The system analyzes stored data for each field and is able to show the necessity of watering to the user in five levels. The system is also able to show various stored data in timeline chart form. The user and coach can compare or analyze these data via a web interface. A test site was built at a Satsuma mandarin field at Kumano in Mie Prefecture, Japan using the framework, and farm workers monitor in the area used and evaluated the system.
Laser cooling and trapping of atoms (2003)

A review is presented of some of the principal techniques of laser cooling and trapping that have been developed during the past 20 years. Its approach is primarily experimental, but its quantitative descriptions are consistent in notation with most of the theoretical literature. It begins with a simplified introduction to optical forces on atoms, including both cooling and trapping. Then its three main sections discuss its three selected features, (1) quantization of atomic motion, (2) effects of the multilevel structure of atoms, and (3) the effects of polychromatic light. Each of these features is an expansion in a different direction from the simplest model of a classical, two-level atom moving in a monochromatic laser field.
The NPro Product of Bovine Viral Diarrhea Virus Inhibits DNA Binding by Interferon Regulatory Factor 3 and Targets It for Proteasomal Degradation (2006)

Bovine viral diarrhea virus (BVDV) is a pestivirus that can establish a persistent infection in the developing fetus and has the ability to disable the production of type I interferon. In this report, we extend our previous observations that BVDV encodes a protein able to specifically block the activity of interferon regulatory factor 3 (IRF-3), a transcription factor essential for interferon promoter activation, by demonstrating that this is a property of the N-terminal protease fragment (NPro) of the BVDV polyprotein. Although BVDV infections cause relocalization of cellular IRF-3 from the cytoplasm to the nucleus early in infection, NPro blocks IRF-3 from binding to DNA. NPro has the additional property of targeting IRF-3 for polyubiquitination and subsequent destruction by cellular multicatalytic proteasomes. The autoprotease activity of NPro is not required for the inhibition of type I interferon induction or the targeting of IRF-3 for degradation.
A Recollection of Margaret H'Doubler's Class Procedure: An Environment for the Learning of Dance (1976)

In 1917 Margaret H'Doubler, on a year's leave from the University of Wisconsin, had been given a difficult assignment. Blanche Trilling, her chairman, had asked if she would search the dance studios in New York City for a kind of dance activity “worth a college woman's time.” Studying philosophy that year at Columbia University, Margaret H'Doubler had complied with pronounced lack of enthusiasm, her predominant interest being in teaching basketball. Her time nearly expired, she found herself, however, suddenly excited when, at the music school of Alys Bentley, she saw children on the floor moving in their own ways to a stimulus provided by the teacher. “Of course,” she recounted later, “Get on the floor where you are relieved from the pull of gravity … and see what the natural, structural movements are.” She continued to visit Miss Bentley's studio during the short time that remained and began to organize a course in “new dancing” to be offered almost immediately in summer school in Madison.
C–H Arylation of Heterocyclic <i>N</i>-Oxides Through <i>in Situ</i> Diazotisation Of Anilines without Added Promoters: A Green And Selective Coupling Process (2016)

A green and selective method for the generation of bi-aryl compounds through C—H arylation of heterocyclic N-oxides is presented in which the addition of ascorbic acid as a promoter is not required for either the generation of an aryldiazonium species or the subsequent arylation. Reaction conditions were optimized through Multivariate Data Analysis, including Orthogonal Projections to Latent Structures (OPLS) and Design of Experiments (DoE) methodologies resulting in further sustainability improvements, and were then applied to a range of substrates to establish the scope and limitations of the process. The reaction was studied using in-situ infra-red spectroscopy and a mechanism is presented that accounts for the available data from this and previous studies. The reaction was also per-formed on a multigram scale, with calorimetry studies to support further scale-up of this promoter-free transformation.
Performance assessment of wind turbine gearboxes using in-service data: Current approaches and future trends (2015)

The evolution of the wind industry in the last decade has not only seen growth in the installed capacity of turbines and innovation within the industry, but has also seen an increase in research activities in this domain. Gearbox field performance, characterised by reliability, availability and maintainability (RAM), has been a major driver in the research domain due to challenges the industry has faced in gearbox design and operations and maintenance. This paper presents a systematic literature review of the current approaches of performance assessment, such as reliability and maintainability analysis of wind turbine gearboxes with a focus on the use of in-service data. The state-of-the-art in literature are discussed and classified according to key research themes, whilst identifying possible gaps due to lack of literature in specific areas. Also, the future trends in gearbox field performance assessment research are explored. In an attempt to close the gaps in one of the areas not covered in literature, an approach for the estimation of gearbox maintainability was presented. Furthermore, a case study on how preventive maintenance of gearbox bearings which can be applied in practice was carried out to demonstrate the importance of the techniques discussed in this article towards meeting industry׳s needs.
Detection of α-globin gene deletion and duplication using quantitative multiplex PCR of short fluorescent fragments (2012)

HBA-QMPSF is able to detect the presence of the common and unusual or unknown α-thalassemia deletions and duplications. It can be used as an initial screening test for α-thalassemia caused by HBA gene copy number alteration.
Different Mechanisms of Protection by Monoclonal and Polyclonal Antibodies during the Course of Herpes Simplex Virus Infection (1991)

Young adult C57BL mice intravaginally inoculated with herpes simplex virus type 1 and passively immunized with a monoclonal antibody directed against herpes simplex virus type 1 glycoprotein gB were shown to be more effectively protected against infection as compared with mice treated with polyclonal immune serum. In contrast to polyclonal antiserum, the monoclonal antibody markedly restricted viral multiplication in the infected mucous membranes. Consequently, skin lesions were completely prevented, and the extent of ganglionic infection was significantly reduced. The mechanism by which a monoclonal antibody, specific to glycoprotein gC, effected protection also differed from that of the hyperimmune serum, since premature latency was not induced. The data provide strong evidence that the mechanisms of protection mediated by antibodies depend on their epitope specificity. The inhibition of active antibody response after passive immunization was inducible by polyclonal antibody only, not by monoclonal antibodies.
Minireview: Ghrelin and the Regulation of Energy Balance--A Hypothalamic Perspective (2001)

The recently discovered hormone, ghrelin, has been recognized as an important regulator of GH secretion and energy homeostasis. Orexigenic and adipogenic ghrelin is produced by the stomach, intestine, placenta, pituitary, and possibly in the hypothalamus. The concentration of circulating ghrelin, principally derived from the stomach, is influenced by acute and chronic changes in nutritional state. To date, most studies focused on the role of ghrelin in GH secretion or its function in complementing leptin action to prevent energy deficits. The potential significance of ghrelin in the etiology of obesity and cachexia as well as in the regulation of growth processes is the subject of ongoing discussions. A large quantity of information based on clinical trials and experimental studies with ghrelin and previously available synthetic ghrelin receptor agonists (GH secretagogues) must now be integrated with a rapidly increasing amount of data on the central regulation of metabolism and appetite. In this overview, we summarize recent findings and strategies on the integration of ghrelin into neuroendocrine networks that regulate energy homeostasis.
Near-Field-Resonance-Enhanced Plasmonic Light Beaming (2010)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> In this paper, we have investigated the enhancement of the plasmonic light beaming efficiency by near-field resonance in different subwavelength metallic slit-groove beaming structures. We show that by selecting the suitable film thickness and separation distance of beaming gratings, the intensity of the near field can be greatly enhanced by resonance, which leads to the increase of the beaming efficiency in the far field. Moreover, we obtain a nanocavity above the nanoslit by integrating a metal nanostrip with a conventional beaming structure. The resonance of this nanocavity can further enhance the near-field intensity and improve the beaming efficiency. </para>
Static correlation visualization for large time-varying volume data (2011)

Finding correlations among data is one of the most essential tasks in many scientific investigations and discoveries. This paper addresses the issue of creating a static volume classification that summarizes the correlation connection in time-varying multivariate data sets. In practice, computing all temporal and spatial correlations for large 3D time-varying multivariate data sets is prohibitively expensive. We present a sampling-based approach to classifying correlation patterns. Our sampling scheme consists of three steps: selecting important samples from the volume, prioritizing distance computation for sample pairs, and approximating volume-based correlation with sample-based correlation. We classify sample voxels to produce static visualization that succinctly summarize the connection among all correlation volumes with respect to various reference locations. We also investigate the error introduced by each step of our sampling scheme in terms of classification accuracy. Domain scientists participated in this work and helped us select samples and evaluate results. Our approach is generally applicable to the analysis of other scientific data where correlation study is relevant.
<i>Salmonella</i>- the ultimate insider.<i>Salmonella</i>virulence factors that modulate intracellular survival (2009)

Salmonella enterica serovar Typhimurium is a common facultative intracellular pathogen that causes food-borne gastroenteritis in millions of people worldwide. Intracellular survival and replication are important virulence determinants and the bacteria can be found in a variety of phagocytic and non-phagocytic cells in vivo. Invasion of host cells and intracellular survival are dependent on two type III secretion systems, T3SS1 and T3SS2, each of which translocates a distinct set of effector proteins. However, other virulence factors including ion transporters, superoxide dismutase, flagella and fimbriae are also involved in accessing and utilizing the intracellular niche.
A picornavirus protein interacts with Ran-GTPase and disrupts nucleocytoplasmic transport (2006)

Active nucleocytoplasmic transport of protein and RNA in eukaryotes depends on the Ran-GTPase system to regulate cargo-receptor interactions. Several viruses, including the RNA picornaviruses, encode factors that alter nuclear transport with the aim of suppressing synthesis of antiviral factors and promoting viral replication. Picornaviruses in the cardiovirus genus express a unique 67-aa Leader protein (L), known to alter the subcellular distribution of IFN regulatory proteins targeted to the nucleus. We report here that L binds directly to Ran and blocks nuclear export of new mRNAs. In Xenopus egg extracts, recombinant L also inhibits mitotic spindle assembly, a RanGTP function crucial to cell-cycle progression. We propose that L inhibits nucleocytoplasmic transport during infection by disrupting the RanGDP/GTP gradient. This inhibition triggers an efflux of nuclear proteins necessary for viral replication and causes IFN suppression. To our knowledge, L is the first viral picornaviral protein to interact directly with Ran and modulate the Ran-dependent nucleocytoplasmic pathway.
Hyperspectral imaging for differentiation of foreign materials from pinto beans (2015)

Food safety and quality in packaged products are paramount in the food processing industry. To ensure that packaged products are free of foreign materials, such as debris and pests, unwanted materials mixed with the targeted products must be detected before packaging. A portable hyperspectral imaging system in the visible-to-NIR range has been used to acquire hyperspectral data cubes from pinto beans that have been mixed with foreign matter. Bands and band ratios have been identified as effective features to develop a classification scheme for detection of foreign materials in pinto beans. A support vector machine has been implemented with a quadratic kernel to separate pinto beans and background (Class 1) from all other materials (Class 2) in each scene. After creating a binary classification map for the scene, further analysis of these binary images allows separation of false positives from true positives for proper removal action during packaging.
Real‐time MR temperature mapping of rabbit liver in vivo during thermal ablation (2003)

It has been shown that quantitative MRI thermometry using the proton resonance frequency (PRF) method can be used to noninvasively monitor the evolution of tissue temperature, and to guide minimally-invasive tumor ablation based on local hyperthermia. Although hepatic tumors are among the main targets for thermal ablation, PRF-based temperature MRI of the liver is difficult to perform because of motion artifacts, fat content, and low T(*) (2). In this study the stability of real-time thermometry was tested on a clinical 1.5 T scanner for rabbit liver in vivo. The fast segmented EPI principle was used together with respiratory gating to limit respiratory motion artifacts. Lipid signal suppression was achieved with a binomial excitation pulse. Saturation slabs were applied to suppress artifacts due to flowing blood. The respiratory-gated MR thermometry in the rabbit liver in vivo showed a standard deviation (SD) of 1-3 degrees C with a temporal resolution of 3 s per slice and 1.4 mm x 1.9 mm spatial resolution in plane (slice thickness = 5 mm). The method was used to guide thermal ablation experiments with a clinical infrared laser. The estimated size of the necrotic area, based on the thermal dose calculated from MR temperature maps, corresponded well with the actual lesion size determined by histology and conventional MR images obtained 5 days posttreatment. These results show that quantitative MR temperature mapping can be obtained in the liver in vivo, and can be used for real-time control of thermal ablation and for lesion size prediction.
[The use of cassava starch in the formulation of gelatin capsules]. (1994)

Cassava, corn and potato starch were, after storage at various conditions of temperature and relative humidity, mixed with a model drug phenacetin. The powder mixtures were filled into hard gelatin capsules. The powder beds containing 20% and 50% starch do not disintegrate within 2 hours. The shortest disintegration time was observed for the mixture containing 80% potato starch. The dissolution rate of phenacetin depends on the porosity of the powder bed filled and the kind of starch used. Potato starch improves the availability of the drug. The disintegration action of corn and cassava starch are almost identical.
An Efficient Passenger-Hunting Recommendation Framework With Multitask Deep Learning (2019)

Using large-scale GPS trajectory data to improve taxi services has recently attracted much attention in Internet of Things and smart city communities. In this paper, we use a large-scale GPS trajectory dataset generated by over 12 000 taxis in a period of three months in Shanghai, China, and present an efficient passenger-hunting recommendation framework with the multitask deep learning paradigm. This framework contains two modules: 1) offline training of passenger-hunting recommendation model (OT-PHRM) and 2) online application of passenger-hunting recommendation model (OA-PHRM). The module OT-PHRM mainly includes two deep convolutional neural networks (DCNNs) and uses the multitask learning strategy. The first DCNN realizes the region prediction for picking up passengers, while the second DCNN uses the weight-sharing structure to predict the levels of road congestion and earnings of carrying passengers. In particular, for the input of two DCNNs, we not only consider contextual features of taxi driving, region features and valuable statistical features, but also combine individual features into meaningful ones. In the module OA-PHRM, we propose DL-PHRec, which calculates three prediction values using two trained DCNNs in OT-PHRM in real time, and then recommends a personal ranking-list of regions to each taxi driver according to their scores. The experimental results show the feasibility and effectiveness of our recommendation framework.
Essential tremor and buccolinguofacial dyskinesias (1982)

Essential tremor (ET) has been described in association with major dyskinesias, such as dystonia musculorum deformans, torticollis, and dystonic writer's cramp. In a series of 104 patients affected by ET, the incidence of buccolinguofacial dyskinesias (BLFD) has been investigated, including a comparison with another dyskinesia involving the facial muscles--mimic muscle tremor. The association of ET and BLFD may be casual; however, on the other hand, it might suggest the existence of a subgroup of patients suffering from a non-specific sign of motor control impairment, such as ET, in which other non-specific disorders of voluntary movement, such as BLFD, can gradually develop, particularly late in life.