gebhart/patrepeval huggingface dataset:
Inhibition of PCSK9 Transcription by Berberine Involves Down-regulation of Hepatic HNF1α Protein Expression through the Ubiquitin-Proteasome Degradation Pathway (2014)

Our previous in vitro studies have identified hepatocyte nuclear factor 1α (HNF1α) as an obligated trans-activator for PCSK9 gene expression and demonstrated its functional involvement in the suppression of PCSK9 expression by berberine (BBR), a natural cholesterol-lowering compound. In this study, we investigated the mechanism underlying the inhibitory effect of BBR on HNF1α-mediated PCSK9 transcription. Administration of BBR to hyperlipidemic mice and hamsters lowered circulating PCSK9 concentrations and hepatic PCSK9 mRNA levels without affecting the gene expression of HNF1α. However, hepatic HNF1α protein levels were markedly reduced in BBR-treated animals as compared with the control. Using HepG2 cells as a model system, we obtained evidence that BBR treatment let to accelerated degradation of HNF1α protein. By applying inhibitors to selectively block the ubiquitin proteasome system (UPS) and autophagy-lysosomal pathway, we show that HNF1α protein content in HepG2 cells was not affected by bafilomycin A1 treatment, but it was dose-dependently increased by UPS inhibitors bortezomib and MG132. Bortezomib treatment elevated HNF1α and PCSK9 cellular levels with concomitant reductions of LDL receptor protein. Moreover, HNF1α protein displayed a multiubiquitination ladder pattern in cells treated with BBR or overexpressing ubiquitin. By expressing GFP-HNF1α fusion protein in cells, we observed that blocking UPS resulted in accumulation of GFP-HNF1α in cytoplasm. Importantly, we show that the BBR reducing effects on HNF1α protein and PCSK9 gene transcription can be eradicated by proteasome inhibitors. Altogether, our studies using BBR as a probe uncovered a new aspect of PCSK9 regulation by ubiquitin-induced proteasomal degradation of HNF1α.
Nimodipine levels in gerbil brain following parenteral drug administration (1985)

Nimodipine binding to the particulate fraction of gerbil brain homogenate was characterized using tritiated (3H)-nimodipine as the radioactive ligand. Binding was monophasic and saturable, with the apparent affinity constant (KD) = 0.4 nM and the maximum number of binding sites (Bmax) = 12 nmol/kg wet wt. A competitive binding assay was validated for the measurement of nimodipine using gerbil brain as the source of receptors for the drug. Binding characteristics were sufficiently similar in specimens from different animals to allow the use of homogenates from individual animals as the source of both membrane-binding sites and competing ligand. Nimodipine could be detected in the brains of animals sacrificed soon after drug injection, and reached a peak level within 15 minutes. Brain drug level at a given time was a linear function of dose administered. One hour after a 1-mg/kg dose, the level of drug measured in brain was approximately 100 nmol/kg wet wt, more that 200 times the KD. Sufficient drug to mediate a maximal pharmacological effect accumulated in brain even after a dose of only 0.25 mg/kg. Thus, in this species, effective tissue nimodipine levels may be achieved at doses which minimize the risk of systemic hypotension.
New Berthing Support System (NBSS) Using High Accuracy Differential GPS - Application to Safety Navigation Management (2009)

The New Berthing Support System (NBSS) will enable position fix-accuracy to within 10cm. It is global and is an effective system to assist LNG tankers, VLCC and other dangerous goods carriers for their safe berthing and entering/leaving port. The system consists of an on-board, maritime mobile station and a base station shore-side. A major function of the mobile station is to record data, sound the alarm on such as course deviation, the monitoring of the velocity component and the position of an object vessel. The major function of the base station is in the management of safe navigation, and to transfer weather at sea information and alike to the mobile station
A computer-assisted cell identification system. (1984)

A computer and optical microscope image processing system for the acquisition, editing and analysis of quantitative, multivariate, cell nuclear data is described. The experimental equipment and methods are discussed, the mathematics used are presented, and the additions, modifications and deletions to the TICAS 11/45 set of computer programs for the analysis of the data are outlined.
C‐terminal specific protein degradation: Activity and substrate specificity of the Tsp protease (1995)

The activity of Tsp, a periplasmic endoprotease of Escherichia coli, has been characterized by assaying the cleavage of protein and peptide substrates, determining the cleavage sites in several substrates, and investigating the kinetics of the cleavage reaction. Tsp efficiently cleaves substrates that have apolar residues and a free alpha-carboxylate at the C-terminus. Tsp cleaves its substrates at a discrete number of sites but with rather broad primary sequence specificity. In addition to preferences for residues at the C-terminus and cleavage sites, Tsp displays a preference for substrates that are not stably folded: unstable variants of Arc repressor are better substrates than a hyperstable mutant, and a peptide with little stable structure is cleaved more efficiently than a protein substrate. These data are consistent with a model in which Tsp cleavage of a protein substrate involves binding to the C-terminal tail of the substrate, transient denaturation of the substrate, and then recognition and hydrolysis of specific peptide bonds.
Clinically‐based determination of safe DNAemia cutoff levels for preemptive therapy or human cytomegalovirus infections in solid organ and hematopoietic stem cell transplant recipients (2004)

Transplantation Centers using human cytomegalovirus (HCMV) antigenemia-based preemptive therapy will need to replace in the near future the antigenemia assay with a more standardized and automatable assay, such as a molecular assay quantifying HCMV DNA in blood (DNAemia). Thus, in view of replacing antigenemia with clinically safe cutoff values, DNAemia levels corresponding to antigenemia cutoffs guiding HCMV preemptive therapy were determined retrospectively in solid organ and hematopoietic stem cell transplant recipients (HSCTR) using an "in-house" quantitative PCR (QPCR) method. Since preemptive therapy had prevented appearance of HCMV disease in all patients tested, DNA cutoffs determined retrospectively had to be considered as safe clinically as antigenemia cutoffs used prospectively. However, in solid organ transplant recipients (SOTR), initiating preemptive therapy upon an antigenemia cutoff of 100 pp65-positive leukocytes, a DNAemia cutoff of 300,000 copies/ml blood had positive and negative predictive values of >90%, indicating that a DNAemia cutoff could achieve, in terms of prevention of HCMV disease, the same clinical results as the antigenemia cutoff. In HSCTR, initiating preemptive therapy upon first antigenemia positivity, a DNAemia cutoff of 10,000 copies/ml blood had a positive predictive value of >90%, indicating that the great majority of patients treated under the antigenemia guidance would have been treated also using this DNA cutoff. On the other hand, the negative predictive value of 28.6% indicated that two out of three HSCTR had been treated under the antigenemia guidance having the same levels of viral DNA as the untreated patients. The data suggest that a quantitative cutoff could be adopted as a guiding criterion for preemptive therapy also in HSCTR. Regression analysis allowed to determine the DNAemia (corresponding to QPCR) cutoff values for two commercial assays tested both in solid organ and HSCTR. Retrospective DNAemia cutoff values will be verified for safety in prospective trials.
Homojunction lead-tin-telluride diode lasers with increased frequency tuning range (1977)

Homojunction lead-tin-telluride (Pb <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1-x</inf> Sn <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</inf> Te) diode lasers with a CW frequency tuning range of 216 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> (near <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">\lambda = 8 \mu</tex> m) were fabricated from a Te-rich, ingot-nucleated crystal. The increased frequency tuning range is attributed to a reduced threshold Current density, which allows a higher operating temperature. At a heat-sink temperature of 11.2 K, the threshold current density (J <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</inf> ) is 212 A cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-2</sup> . The highest heat-sink temperature recorded for these lasers is 61 K with a corresponding J <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</inf> of <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1.75 \times 10^{3}</tex> A cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-2</sup> . The longest, continuous current tuning (at 17 K) of one mode is 2.4 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> . At a constant current, the temperature tuning of the same mode is 0.9 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> . The lasers generally emit a single mode for current levels below 125 percent of J <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">th</inf> . Single-mode, single-ended output powers up to 0.9 mW have been recorded.
The<i>n</i>-dimensional analogue of the catenary: existence and nonexistence (1990)

We study "heavy" A?-dimensional surfaces suspended from some prescribed (n -1) -dimensional boundary data. This leads to a mean curvature type equation with a non-monotone right hand side. We show that the equation has no solution if the boundary data are too small, and, using a fixed point argument, that the problem always has a smooth solution for sufficiently large boundary data.
Identification of Cell Surface Markers Glypican-4 and CD200 That Differentiate Human Corneal Endothelium From Stromal Fibroblasts (2013)

Taken together, the anti-GPC4 and anti-CD200 antibodies can be useful for purification and identification of HCECs in cultures containing stromal fibroblasts.
Time-encoded speech (1978)

A new method of digitising speech waveforms is described, based on the comparison of successive segments of the waveform with a suitably stored catalogue of possible distinct shapes.
Isoelectric precipitation of soy protein. II. Kinetics of protein aggregate growth and breakage (1983)

Protein aggregate growth and breakage in agitated suspensions are modeled. The model includes growth of particles by a turbulent collision mechanism and breakage by a hydrodynamic shear mechanism. In the model, breakage results in the splitting of the particles into several small fragments. The model parameters are a growth rate constant and a breakage rate constant. Aggregate size distributions were measured with a Coulter counter and the data interpreted using a population balance that governs the steady-state particle size distribution in a continuous stirred tank reactor. Effects of changes in the operating variables pH, concentration, mean residence time, ionic strength, and mixing power input on the model kinetic parameters are investigated.
Basal Release of Nitric Oxide Is Decreased in the Coronary Circulation in Patients With Heart Failure (1997)

It is unknown whether basal release of endothelium-derived nitric oxide in the coronary artery is altered in heart failure in humans. The aim of the present study was to evaluate the effect of inhibition of nitric oxide synthesis on basal tone of the conduit and resistance coronary arteries in awake patients. Coronary blood flow velocity (Doppler guide wire) and coronary arterial diameter (quantitative coronary angiography) were measured in 14 patients with heart failure caused by nonischemic left ventricular dysfunction (7 idiopathic dilated cardiomyopathy and 7 valvular insufficiency) and 7 patients with normal ventricular function (controls). Intracoronary N(G)-monomethyl-L-arginine (L-NMMA), an inhibitor of nitric oxide synthesis, at graded doses decreased coronary blood flow in both groups. However, the magnitude of flow reduction was smaller in patients with heart failure than in control patients (P<.0001). The magnitude of coronary blood flow reduction in response to L-NMMA inversely correlated to indexes of left ventricular contractile function (P<.01) but was not affected by the cause of heart failure. Constriction of the large epicardial coronary artery with L-NMMA also tended to be attenuated in patients with heart failure. In summary, vasoconstricting response to L-NMMA was blunted in the coronary resistance artery in heart failure in vivo. These findings suggest that basal release of nitric oxide in the coronary circulation is decreased in patients with heart failure.
Oral Relugolix for Androgen-Deprivation Therapy in Advanced Prostate Cancer (2020)

In this trial involving men with advanced prostate cancer, relugolix achieved rapid, sustained suppression of testosterone levels that was superior to that with leuprolide, with a 54% lower risk of major adverse cardiovascular events. (Funded by Myovant Sciences; HERO ClinicalTrials.gov number, NCT03085095.).
Design of index-coupled DEB lasers with reduced longitudinal spatial hole burning (1991)

Methods for reducing or eliminating longitudinal spatial hole burning in antireflection-coated, index-coupled distributed feedback (DFB) lasers are discussed. It is shown that elimination of spatial hole burning in DFB lasers can be achieved by the introduction of well-chosen variations of coupling strength and/or internal absorption in the longitudinal direction. It is shown that a theoretical laser structure with uniform power can always be obtained by choosing two arbitrary functions. The authors describe some of the more basic solutions with uniform power. Practical approximations of these structures, their numerical modeling, and some methods to fabricate them are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Sensor cooling by direct blowdown of a coolant (1995)

This article describes results of a proof-of-concept study on cooling of the optical components in a long wavelength infrared (LWIR) sensor using direct blowdown of R23 refrigerant. This technique allows on-demand cooling of optics in a sensor system with an extremely simple and lightweight system. The thermodynamic properties of certain refrigerants such as R23 allow a direct expansion from a high pressure and ambient temperature (300 K) to saturated two-phase conditions at a low pressure without precooling. Refrigerant saturation temperatures between the triple point (118 K) and the normal boiling point (191 K) can be obtained depending upon the level of vacuum available. Experimental results are presented for a 15-cm-diam primary mirror intended for use in a LWIR sensor. The mirror (weighing between 500-750 g) was cooled using direct blowdown of R23 to temperatures of less than 140 K. Cooling times were on the order of 2-3 min. A onedimensional thermohydraulic model was developed to model the cooldown process. Good correlation was obtained between the model and the experimental results. Further system optimization has been undertaken using the model to minimize system mass for a given aperture sensor optical system and cooling requirement.
A Three-Dimensional Numerical Study of Rotational Detonation in an Annular Chamber (2009)

Detonation waves successively rotating in an annular chamber are numerically investigated to understand an overall ∞owfleld structure in the combustion chamber of an engine and its basic operation with a continuous fuel injection. This study leads to further investigation into continuously rotating detonation wave, which is the backbone in developing a rotating detonation-based propulsion system. A computational code developed is based on multi-dimensional Euler equations with source terms due to chemical reactions. Spatial terms in governing equations are discretized with a flnite volume method and a MUSCLbased Roe scheme, while temporal terms are discretized with a second-order, three-step Runge-Kutta method. Source terms are treated with a time-operator splitting method in order to isolate stifiness. The detonation is modeled with the one-step chemical reaction of a hydrogen and air mixture. A detailed ∞owfleld structure including detonation properties is presented in two- and three-dimensional annular chamber. The propulsive parameters of a rotational detonation engine are evaluated and its comparison of one- and two-waved detonation engine is performed in a three-dimensional chamber.
The biophotonics platform gaining momentum (2009)

Journal of Biophotonics can look back on a very successful year 2009. In the second year since its foundation in 2008 the frequency of publication was increased to 12 issues. Among these 12 issues we had five topical issues edited by scientists leading in their fields, reporting about exciting biophotonic topics such as:
Health and the health services (1995)

In this society, there is a tendency to see health as very much an individual issue. This leads to an emphasis on the biological causes of ill-health together with the recent stress on personal factors such as smoking and weight. Improvements in health are seen as resulting from intervention by doctors at the level of the individual, or changes in personal life-style. This chapter looks at health and health care from a different point of view, focusing on social, political, economic and environmental aspects of health. It will become apparent that health is not a purely personal matter, but an area of considerable national and social concern.
Stabilisation of mode-locked pulses using travelling-wave semiconductor laser amplifier (1990)

Numerical and experimental results show, for the first time, that a saturated travelling-wave laser amplifier (TWLA) can be used to amplify and limit the amplitudes of pulses from an actively mode-locked semiconductor laser while removing unwanted secondary pulses.
Block BFGS Methods (2018)

We introduce a quasi-Newton method with block updates called block BFGS. We show that this method, performed with inexact Armijo--Wolfe line searches, converges globally and $Q$-superlinearly under the same convexity assumptions as BFGS. We also show that block BFGS is globally convergent to a stationary point when applied to nonconvex functions with bounded Hessian, and discuss other modifications for nonconvex minimization. Numerical experiments comparing block BFGS, BFGS, and gradient descent are presented.
Miscibility of polymethacrylate/poly(vinyl chloride) blends (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMiscibility of polymethacrylate/poly(vinyl chloride) blendsJerome F. Parmer, L. Charles Dickinson, J. C. W. Chien, and Roger S. PorterCite this: Macromolecules 1989, 22, 3, 1078–1083Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March 1989https://pubs.acs.org/doi/10.1021/ma00193a014https://doi.org/10.1021/ma00193a014research-articleACS PublicationsRequest reuse permissionsArticle Views477Altmetric-Citations80LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
PTFE-encapsulated endovascular stent-graft for transjugular intrahepatic portosystemic shunts: experimental evaluation. (1997)

This PTFE-encapsulated stent-graft is biocompatible and safe to place. It markedly improves TIPS patency, providing almost uninterrupted, unimpeded patency in this model.
ELECTRON MICROSCOPIC DEMONSTRATION OF INDOXYL ACETATE ESTERASE IN RAT LIVER (1970)

Indoxyl acetate esterase was demonstrated in rat liver by an azo-indoxyl method at the fine structural level. The present study on the intracellular localization of esterase activity suggests that the esterase activity in centrifugal fractions might not be the contamination of the microsomal fraction. 10-3 M sodium fluoride completely suppressed the staining of the cytoplasmic membranous structure, but did not suppress the stain of the droplets, exhibiting the so-called peribiliary activity of type A esterase.
THE CLASSIFIED ADS (1994)

Deadlines: O rders for regular classified advertisements must reach the ACRL office on or before the second of the month preceding publication of the issue (e.g., September 2 for the O ctober issue). Should this date fall on a weekend or holiday, ads will be accepted on the next
Intranasal Lidocaine for Cluster Headache (1995)

Thirty male patients with cluster headache were given 4% lidocaine solution to use intranasally as an abortive therapy. Four sprays of lidocaine were used ipsilateral to the pain, and two more were used, if necessary. Twenty-seven percent of the men reported moderate relief, 27% obtained mild relief, and 46% stated that they had no relief from the lidocaine, Side effects were minimal. In this study, intranasal lidocaine was only a marginally helpful therapy for cluster headache. However, because of the ease of administration and lack of side effects, lidocaine may remain worthwhile as an adjunctive medication.