gebhart/patrepeval huggingface dataset:
[<sup>32</sup>P]2‐iodo‐<i>N</i><sup>6</sup>‐methyl‐(<i>N</i>)‐methanocarba‐2′‐deoxyadenosine‐3′,5′‐bisphosphate ([<sup>32</sup>P]MRS2500), a novel radioligand for quantification of native P2Y<sub>1</sub> receptors (2005)

Analysis of the P2Y family of nucleotide-activated G-protein-coupled receptors has been compromised by the lack of selective high-affinity, high-specific-radioactivity radioligands. We have pursued quantification of the P2Y1 receptor through the development of a series of selective P2Y1 receptor antagonists. Recently, we synthesized 2-iodo-N6-methyl-(N)-methanocarba-2'-deoxyadenosine 3',5'-bisphosphate (MRS2500), a selective, competitive antagonist that exhibits a Ki of 0.8 nM in competition-binding assays with [3H]MRS2279. A 3'-monophosphate precursor molecule, MRS2608, was radiolabeled at the 5' position with 32P using polynucleotide kinase and [gamma32P]ATP to yield [32P]MRS2500. [32P]MRS2500 bound selectively to Sf9 insect cell membranes expressing the human P2Y1 receptor (Sf9-P2Y1), but did not detectably bind membranes expressing other P2Y receptors. P2Y1 receptor binding to [32P]MRS2500 was saturable with a KD of 1.2 nM. Agonists and antagonists of the P2Y1 receptor inhibited [32P]MRS2500 binding in Sf9-P2Y1 membranes with values in agreement with those observed in functional assays of the P2Y1 receptor. A high-affinity binding site for [32P]MRS2500 (KD=0.33 nM) was identified in rat brain, which exhibited the pharmacological selectivity of the P2Y1 receptor. Distribution of this binding site varied among rat tissues, with the highest amount of binding appearing in lung, liver, and brain. Among brain regions, distribution of the [32P]MRS2500 binding site varied by six-fold, with the highest and lowest amounts of sites detected in cerebellum and cortex, respectively. Taken together, these data illustrate the synthesis and characterization of a novel P2Y1 receptor radioligand and its utility for examining P2Y1 receptor expression in native mammalian tissues.
The functional form of the erythropoietin receptor is a 78-kDa protein: correlation with cell surface expression, endocytosis, and phosphorylation. (1993)

An abundant 70- to 78-kDa form of the erythropoietin receptor (EPOR) was observed in HC-D57 murine erythroleukemia cells deprived of erythropoietin (EPO). In contrast to the 64- and 66-kDa EPOR proteins, these high molecular mass forms of EPOR (hmm-EPOR) correlated well with the number of binding sites and endocytosis of EPO. The hypothesis that hmm-EPOR are more highly glycosylated forms of the EPOR, appear on the cell surface, and represent at least one component of the biologically active EPOR was tested. Consistent findings were as follows. (i) Only hmm-EPOR increased following withdrawal of EPO from HC-D57 cells, correlating with a 10-fold increase in binding of 125I-labeled EPO. In addition, the EPO-dependent downregulation of 125I-EPO binding and disappearance of hmm-EPOR occurred in parallel while the amount of 66-kDa EPOR did not change. (ii) The 78-kDa EPOR was detected in COS cells expressing EPOR cDNA. (iii) Probing of the intact surface of these cells with anti-NH2-terminal antibody recovered only the 78-kDa EPOR. (iv) Enzymatic deglycosylation and dephosphorylation showed that hmm-EPOR apparently resulted from additional N-linked glycosylation of a 62-kDa EPOR. (v) The hmm-EPOR turnover in HC-D57 cells was accelerated 12-fold in the presence of EPO (half-life changed from 3 hr to 15 min). (vi) Anti-phosphotyrosine antiserum detected an EPO-dependent phosphorylation of the 78-kDa EPOR. The kinetics of tyrosine phosphorylation of a 97-kDa protein correlated with the occupancy and internalization of hmm-EPOR. In summary, we suggest that the 78-kDa EPOR is directly involved in the initial biological actions of EPO.
Modulation of human lymphoblastoid interferon activity by melatonin in metastatic renal cell carcinoma. A phase II study (1994)

Response rate and toxic effects observed during this study warrant additional randomized studies to define the role of MLT's concomitant administration in the clinical response to IFN in metastatic RCC.
Recent progress in silicon solar cells (2002)

The present paper reports two recent independently confirmed results for silicon solar cell efficiency. An improved efficiency for an individual silicon cell of 24.5% is reported, the highest ever, as is 19.8% efficiency for a multicrystalline silicon cell, the latter representing a 6.5% relative improvement over the previously best result. Not only does the latter result for this relatively low quality material closely approach the 20% efficiency mark, once thought to be a limit on silicon cell performance regardless of multicrystalline material quality, but it also shows the potential for such multicrystalline material exceeding the performance capacity of standard crystalline Czochralski material.
Image segmentation approach to extract colon lumen through colonic material tagging and hidden Markov random field model for virtual colonoscopy (2002)

Virtual colonoscopy provides a safe, minimal-invasive approach to detect colonic polyps using medical imaging and computer graphics technologies. Residual stool and fluid are problematic for optimal viewing of the colonic mucosa. Electronic cleansing techniques combining bowel preparation, oral contrast agents, and image segmentation were developed to extract the colon lumen from computed tomography (CT) images of the colon. In this paper, we present a new electronic colon cleansing technology, which employs a hidden Markov random filed (MRF) model to integrate the neighborhood information for overcoming the non-uniformity problems within the tagged stool/fluid region. Prior to obtaining CT images, the patient undergoes a bowel preparation. A statistical method for maximum a posterior probability (MAP) was developed to identify the enhanced regions of residual stool/fluid. The method utilizes a hidden MRF Gibbs model to integrate the spatial information into the Expectation Maximization (EM) model-fitting MAP algorithm. The algorithm estimates the model parameters and segments the voxels iteratively in an interleaved manner, converging to a solution where the model parameters and voxel labels are stabilized within a specified criterion. Experimental results are promising.
Comparative performance of various automotive navigation technologies (1993)

The first practical in-vehicle automotive navigation systems (introduced in 1985) utilized dead reckoning combined with digital map-matching to keep track of vehicle positions and to show them in real time on digital map displays. This dead-reckoning/map-matching technology has advanced considerably. In recent years, other vehicle location and tracking technologies have emerged including Global Positioning Satellites (GPS), differential GPS, radio-location (resembling Loran or inverse Loran), cellular phone tracking, and electronic benchmarks (or signposts) in or near the roads. Loran C is also under consideration. The advantages and limitations of the various vehicle location and tracking techniques are described, and how their limitations can be overcome by combining or improving techniques is discussed. Tradeoffs between features, performance, and cost are presented for some specific applications.
Deposition of refractory metal films by rare-gas halide laser photodissociation of metal carbonyls (1986)

Films of Cr, Mo, and W on quartz, Pyrex, Suprasil, and Al substrates were deposited by photodissociation of the respective hexacarbonyls using focused and pulsed radiation from rare-gas halide lasers. Cr was deposited by dissociation of Cr(CO)6 using XeF (308 nm), KrF (249 nm), and ArF (193 nm) lasers. Mo and W were deposited from their respective hexacarbonyls at 249 and 193 nm. Pulse energies varied between 8 and 12 mJ. Pulse rates of 10–60 Hz were used. The pulse duration was about 10 ns. Depositions with substrates both parallel and perpendicular to the excimer radiation were attempted. Only in the case of perpendicular configuration were strongly adherent films observed. The deposition rates for thicknesses up to 3000 Å appeared to be independent of the pulse rate for all three metals. The films exhibited strong adhesion to the substrate. Scanning electron microscope photographs of the films revealed the presence of continuous metal layers. Auger and x-ray analyses of the films indicated contamination from carbon and oxygen. The source of these impurities is most likely to be CO produced in the decarbonylation of the parent hexacarbonyl. Adhesion to the substrate is apparently enhanced by laser stimulated generation of strong binding sites on the surface.
Expression of two "immediate early" genes, Egr-1 and c-fos, in response to renal ischemia and during compensatory renal hypertrophy in mice. (1990)

To identify specific genetic regulatory mechanisms associated with renal ischemia, we measured the accumulation of Egr-1 and c-fos mRNAs in the mouse kidney after occlusion of the renal artery and reperfusion. At 1 h after right nephrectomy and arterial occlusion of the contralateral kidney for 10 or 30 min, Egr-1 mRNA levels were three to five times greater in these kidneys as compared with those in control animals that had sustained unilateral nephrectomy alone and were much greater than levels in the normal organ. Whether ischemia was imposed for 10 or for 30 min, renal Egr-1 mRNA contents were equivalent and remained elevated after 24 h of reperfusion subsequent to 30 min of ischemia. Although c-fos mRNA also accumulated in response to ischemia and reperfusion, the pattern differed from that of Egr-1 in that c-fos mRNA content varied with the duration of ischemia and was undetectable 24 h after injury. Contralateral nephrectomy was not necessary to see the marked accumulation of Egr-1 and c-fos mRNAs with unilateral ischemia. Reflow was necessary, however, since only minimal sequence accumulation occurred by the end of the ischemic period. After left uninephrectomy alone, Egr-1 mRNA levels in the remaining kidney were maximal 30 min after surgery, but were not detectable thereafter; c-fos mRNA levels did not change after unilateral nephrectomy. Differential expression of early growth-related genes implicated in transcriptional activation may influence tissue recovery after renal ischemia.
Es posible ayudar a mantener la sensación de control de la familia sobre el cuidado del familiar críticamente enfermo (2012)

Justificacion. El desarrollo y la modernizacion tecnologica ha propiciado un aumento de la complejidad de los procesos atendidos en las UCIs, encaminandose la atencion en este contexto a salvar vidas incluso cuando la esperanza es muy remota. Sin embargo los pacientes parecen sufrir por insuficiente comunicacion, alteracion del sueno y falta de empatia del equipo. Existe una evolucion de los profesionales en el dominio tecnico, pero no del humano.
Son muchas y diversas las creencias y fantasias existentes sobre este contexto y podrian ser las enfermeras quienes establecieran una adecuada comunicacion con los familiares sobre rutinas, aparatos y cuidados, ofreciendo el necesario apoyo emocional y espiritual, cuidado sin duda complejo para implantar por las rutinas establecidas y apegadas.
Estudios enfermeros realizados sobre familias de pacientes ingresados en UCI han identificado discursos significativos, referidos a la dificultades de los profesionales para relacionarse con la familia, en las dimensiones emocional, relacional y organizacional.
Otro trabajo de enfermeria verifico el influjo sobre la atencion del paciente en la grabacion de voz de familiares significativos con fondo musical, sobre los signos vitales y la expresion facial de los pacientes en coma fisiologico o inducido.

Objetivos. Identificar los significados comunes en los mensajes grabados por los familiares de pacientes en estado de coma ingresados en UCIs.

Diseno. Estudio descriptivo, transversal y exploratorio con abordaje cualitativo de analisis del contenido de mensajes grabados por los participantes para llegar a entender sus expectativas y necesidades.

Lugar. El estudio fue realizado en dos UCIs (Trauma y Clinica) del Hospital das Clinicas de la Facultad de Medicina Universidad de Sao Paulo (Brasil).

Sujetos. 30 mensajes grabados por los familiares de los pacientes ingresados en UCI.

Recogida de datos. Se dieron instrucciones a los familiares mas significativos para el paciente, con el objetivo de realizar una audio grabacion de su voz sobre un fondo musical preferido por aquel, con una duracion de tres minutos. El contenido del mensaje era dirigido a que le orientaran sobre donde estaba y lo que con el hacian. Ademas se les pedia que transmitieran expresiones afectuosas con una perspectiva optimista.

Analisis de los datos. Se realizo un analisis de contenido de las grabaciones familiares.

Resultados. Se establecieron 7 categorias que se exponen en la tabla 1.

Tabla 1. Categorias del estudio


Conclusiones. El sufrimiento de la familia durante la estancia de un ser querido en UCI produce inseguridad y otros sentimientos y preocupaciones, siendo la esperanza transversal a todas ellas, util para afrontar los cambios, transformaciones, dificultades, aprendizaje y conseguir la fuerzas necesaria en esos momentos para continuar existiendo.
Identificar esos sentimientos puede mejorar e individualizar la atencion de enfermeria, dando el primer paso para ayudarles a superar esa experiencia.
Optisches Zweistrahlverfahren zur Bestimmung von Polyelektrolyten in Wasser und zur Messung der Polymeradsorption an Grenzflächen (1978)

Zur gezielten Steuerung der Stabilitäts- und Koagulationseigenschaften disperser Systeme werden in zunehmendem Maße organische Polyelektrolyte eingesetzt. Die kolloide Stabilität wäßriger Dispersionen wird in vielen Fällen durch Zusatz anionaktiver Polyelektrolyte verbessert (1). Bei der Herstellung von Papier oder bei der Abwasseraufbereitung werden sowohl kationaktive als auch anion- aktive Polyelektrolyte als Retendons- bzw. Flockungs-Hilfsmittel verwendet (1–5).
A review of ANN-based short-term load forecasting models (2002)

Artificial neural networks (ANN) have recently received considerable attention and a large number of publications concerning ANN-based short-term load forecasting (STLF) have appeared in the literature. An extensive survey of ANN-based load forecasting models is given. The six most important factors which affect the accuracy and efficiency of the load forecasters are presented and discussed. The paper also includes conclusions reached by the authors as a result of their research in this area.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Optimization of method for extraction of template of bacteria for randomly amplified polymorphic DNA (2001)

Objective:To obtain optimum template for randomly amplified polymorphic DNA(RAPD). Methods:Four different methods(rod winding, precipitation, boiling and lysis)for extraction of template DNA were used.The length and purity of template DNA and its RAPD profile were observed separately by agarose gel electrophoresis, spectrophotometric scan assays and RAPD reaction.Results:The template DNA (23 kb) with high purity and the same RAPD profile with 2-4 kb DNA fragments were obtained by both rod winding and precipitation method. However,the template DNA (4 kb and 2 kb,respectively) with break and dispersion and low purity was extracted by method of boiling and lysis, and 600-2 000 bp DNA fragments were seen in the similar RAPD profile.Conclusions: Template DNA extracted by rod winding or precipitation method was optimized for RAPD. [
Studies on phosphatidylinositol phosphodiesterase (phospholipase C type) of Bacillus cereus. I. purification, properties and phosphatase-releasing activity. (1976)

A phosphatidylinositol phosphodiesterase from the culture broth of Bacillus cereus, was purified to a homogeneous state as indicated by polyacrylamide gel electrophoresis, by ammonium sulfate precipitation and chromatography with DEAE-cellulose and CM-Sephadex. The enzyme (molecular weight: 29000 +/- 1000) was maximally active at pH 7.2-7.5, AND NOT INFLUENCED BY EDTA, ophenanthroline, monoiodoacetate, p-chloromercuribenzoate or reduced glutathione. The enzyme specifically hydrolyzed phosphatidylinositol, but did not act on phosphatidylcholine, phosphatidylethanolamine and sphingomyelin, under the conditions examined. The products from phosphatidylinositol of enzyme reaction were diacylglycerols and a mixture of myoinositol 1- and 1, 2-cyclic phosphates, suggesting that the enzyme was a phosphatidylinositol-specific phospholipase C. The enzyme released alkaline phosphatase quantitatively from rat kidney slices. A kinetic analysis was made on the release of alkaline phosphatase. The results suggest that phosphatidylinositol-specific phospholipase C can specifically act on plasma membrane of rat kidney slices.
Effect of Gabapentin on the Sensation and Impact of Tinnitus (2006)

Gabapentin is effective in reducing subjective and objective aspects of tinnitus in some individuals, with the best therapeutic response obtained in individuals with associated acoustic trauma.
Monopulse angle estimation with constrained adaptive beamforming using simple mainlobe maintenance technique (2004)

A new monopulse radar system is proposed to overcome the difficulties with conventional monopulse techniques under jamming conditions. The proposed system uses a simple mainlobe maintenance technique based on the estimated direction of the mainlobe jammer. Thereafter, an adaptive array is used to create pattern nulls in the incoming directions of jammers, while maintaining the shape of the mainlobe. As a result, the proposed system can track the target angle without any correction of the adaptive sum and difference beam outputs.
Die Zähigkeit metallischer Diffusionsschutzschichten auf Nickellegierungen und ihre Kennzeichnung (1984)

Abstract Diffusionsschutzschichten gegen Heißgaskorrosion sind vielfach bei niedrigen Temperaturen spröde und werden erst bei höheren Temperaturen zäh. Für das Betriebsverhalten beschichteter Turbinenschaufeln z. B. ist die Kenntnis der Übergangstemperatur vom spröden zum zähen Verhalten von Bedeutung, wobei die Eigenschaften der Schutzschicht vom jeweiligen Trägerwerkstoff beeinflußt werden. ‐ Es wird nun ein Prüfverfahren vorgestellt, mit dem an beschichteten Proben unter mechanischer Beanspruchung das Auftreten erster Anrisse in der Schutzschicht durch Schallemissionsmessungen nachgewiesen und gleichzeitig durch die Aufnahme eines Spannung‐Dehnung‐Schaubildes die relative Lage der Anrißspannung der Schutzschicht und der 0,2 Grenze des Trägerwerkstoffes erfaßt werden. Aus den durchgeführten Versuchen ließen sich Angaben über die Zähigkeit in Abhängigkeit von der Temperatur und anderen Einflußgrößen wie Schichtdicke, Dehngeschwindigkeit und thermischer Vorgeschichte ableiten. Auf diese Weise konnten für drei verschiedene Schutzschichten, dei mit zwei hochwarmfesten Nickellegierungen kombiniert waren, Spröde Zäh‐Übergangstemperaturen bestimmt werden. Weiterhin ergaben sich bis zur höchsten Prüftemperatur von 850°C Spannungs‐ und Dehnungsgrenzen, unterhalb derer die untersuchten Schutzschichten auf den Trägerwerkstoffen rißfrei bleiben.
Synthesis of supertriptycene and two related iptycenes (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of supertriptycene and two related iptycenesKhalil Shahlai and Harold HartCite this: J. Org. Chem. 1991, 56, 24, 6905–6912Publication Date (Print):November 1, 1991Publication History Published online1 May 2002Published inissue 1 November 1991https://pubs.acs.org/doi/10.1021/jo00024a037https://doi.org/10.1021/jo00024a037research-articleACS PublicationsRequest reuse permissionsArticle Views374Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Optical constants of Cu, Ag, and Au revisited (2015)

We have determined the optical constants in the energy range 0.1–6 eV for bulk Cu, Ag, and Au using Kramers–Kronig analysis of previously unpublished reflectance data. The results are compared to those commonly used from the literature.
MMP‐1 Reduced in Organ Cultured Human Skin and Dermal Fibroblasts by Ginger and Curcumin (2009)

Recent studies have shown that ginger extract and curcumin work synergistically to promote wound healing in Temovate‐treated rats. With this in mind, the current study focused on the effects of combined ginger and curcumin on organ cultured human skin and primary dermal fibroblasts. Dermal fibroblasts were treated for a 72‐hour period with a range of ginger and curcumin concentrations, both individually and combined, in low calcium and high calcium media. Biopsy punches from the hip of adult human subjects were organ cultured for 6 days in supportive media and treated with a similar dose range of ginger and curcumin as the fibroblasts. Findings were parallel in fibroblast monolayer and organ cultured skin. The combination of ginger and curcumin protected fibroblasts from cytotoxicity under conditions in which survival did not occur in their absence. Taken together with the previous wound‐healing study, these data support the synergistic action of ginger and curcumin and indicate their potential benefit in "at‐risk" human skin. Work supported by NIH HL07097
일본 정부개발원조(ODA) 연구의 비판적 검토 - 일본 ‘원조외교’ 연구방법의 시론(試論) (2001)

1970년을 전후로 일본의 대외 경제외교가 학술연구 대상으로 주목을 받는다. 1990년대에 들어와 ODA(Official Development Assistance)부문에서 일본은 미국을 능가하는 최대 원조 공여국(Top Donor)이 된다. 이런 과정에서 일본의 경제외교는 세계적인 관심을 끈다. 이에 본론은 첫째로 일본 원조(ODA)외교를 연구하는데...
Fast Synthesis of MOF-5 Microcrystals Using Sol−Gel SiO<sub>2</sub> Nanoparticles (2011)

The work reports on the synthesis of an archetypal metal organic framework (MOF-5) microcrystals with a narrow size distribution using SiO2 nanoparticles with tailored surface chemistry as nucleating agents. The nanoparticles boost the reaction rate by up to an order of magnitude compared to the conventional MOF-5 solvothermal synthesis and can be successfully used as nucleation seeds for the selective growth of MOF-5 on specific substrates. These results are important fundamental advances toward the controlled scale-up of MOF synthesis and directed MOF growth on suitable supports.
Dielectric and Ferroelectric Properties of Ceramics in the Pb(Zn <sub>1/3</sub> Nb <sub>2/3</sub> )O <sub>3</sub> ‐BaTiO <sub>3</sub> ‐PbTiO <sub>3</sub> System (1987)

The use of Pb(Zn 1/3 Nb 2/3 )O 3 ceramics is restricted by the formation of a pyrochlore phase detrimental to both dielectric and piezoelectric properties. Recently it has been shown that a 6 mol% addition of BaTiO 3 to PZN suppresses the formation of pyrochlore phase. Phase relations and dielectric properties of ceramics in the PZN‐BT‐PT system are reported here. Compositions with the perovskite structure, having high dielectric constant and low temperature coefficient of capacitance, have been identified.