gebhart/patrepeval huggingface dataset:
Regulation of TFPIα expression by miR-27a/b-3p in human endothelial cells under normal conditions and in response to androgens (2017)

The increased risk of cardiovascular events in older men is multifactorial, but the significant reduction of testosterone levels has been involved. As this hormone regulates the expression of TFPI by unknown mechanisms, we aimed to evaluate the role of miRNAs in the regulation of TFPIα expression under normal conditions and in response to androgens. In silico studies allowed the selection of 4 miRNAs as potential TFPIα regulators. Only miR-27a/b-3p significantly reduced TFPIα expression in two endothelial cell lines. Luciferase assays demonstrated a direct interaction between miR-27a/b-3p and TFPI 3'UTR. Ex vivo analysis of TFPI and miRNA levels in 74 HUVEC samples from healthy subjects, showed a significant and inverse correlation between TFPI and miR-27a-3p. Moreover, anticoagulant activity of TFPIα from cells supernatants decreased ~30% with miR-27a/b-3p and increased ~50% with anti-miR-27a/b-3p. Interestingly, treatment of EA.hy926 with a physiological dose of dihydrotestosterone (30 nM) significantly increased (~40%) TFPIα expression with a parallel decreased (~50%) of miR-27a/b-3p expression. In concordance, increased levels of miR-27a/b-3p normalized the up-regulation induced by testosterone. Our results suggest that testosterone is a hinge in miR-27/TFPIα regulation axis. Future studies are needed to investigate whether testosterone variations are involved in a miR-27/TFPIα dysregulation that could increase the cardiovascular risk.
New Delivery Systems and Propellants (1999)

The removal of chlorofluorocarbon (CFC) propellants from industrial and household products has been agreed to by over 165 countries of which more than 135 are developing countries. The timetable for this process is outlined in the Montreal Protocol on Substances that Deplete the Ozone Layer document and in several subsequent amendments. Pressured metered dose inhalers (pMDIs) for medical use have been granted temporary exemptions until replacement formulations, providing the same medication via the same route, and with the same efficacy and safety profiles, are approved for human use. Hydrofluoroalkanes (HFAs) are the alternative propellants for CFCs-12 and -114. Their potential for damage to the ozone layer is nonexistent, and while they are greenhouse gases, their global warming potential is a fraction (one-tenth) of that of CFCs. Replacement formulations for almost all inhalant respiratory medications have been or are being produced and tested; in Canada, it is anticipated that the transition to these HFA or CFC-free pMDIs will be complete by the year 2005. Initially, an HFA pMDI was to be equivalent to the CFC pMDI being replaced, in terms of aerosol properties and effective clinical dose. However, this will not necessarily be the situation, particularly for some corticosteroid products. Currently, only one CFC-free formulation is available in Canada - Airomir, a HFA salbutamol pMDI. This paper discusses the in vitro aerosol characteristics, in vivo deposition and clinical data for several HFA pMDIs for which there are data available in the literature. Alternative delivery systems to the pMDI, namely, dry powder inhalers and nebulizers, are briefly reviewed.
Joint measurement- and traffic descriptor-based admission control at real-time traffic aggregation points (2004)

The primary role of admission control is to decide on the amount of traffic accepted into the network so that users conforming to their established traffic contracts achieve predefined performance objectives, e.g. bounded packet loss probability, end-to-end delay. We consider Diffserv networks able to support real-time traffic and we propose a novel framework for admission control that involves both traffic descriptor and measurement-based techniques and we compare its performance with existing approaches. Our simulation results show that the performance of our approach is rather insensitive to variations of the traffic sources. Even when the provided traffic descriptors are as simple as a single value denoting the required peak rate, the proposed scheme achieves satisfactory performance.
Optical and Structural Properties of Eu[sup 2+]-doped (Sr[sub 1−x]Ba[sub x])[sub 2]SiO[sub 4] phosphors (2005)

Europium -doped alkaline earth orthosilicates , are studied as a phosphor for color-tunable white-light-emitting diode. With an increase of Ba ions in , the lattice constants increase, and the emission bands shift to higher energy in a systematic way. This blueshift behavior can be clarified by crystal field strength. Also, the lattice vibration energies from Raman spectra decrease with an increase of Ba ions in . The emission-quenching temperature dependence of the lattice vibration frequency is investigated in terms of a well-known configurational coordinate diagram.
Insights into the Pathology of the α3 Na+/K+-ATPase Ion Pump in Neurological Disorders; Lessons from Animal Models (2016)

The transmembrane Na(+)-/K(+) ATPase is located at the plasma membrane of all mammalian cells. The Na(+)-/K(+) ATPase utilizes energy from ATP hydrolysis to extrude three Na(+) cations and import two K(+) cations into the cell. The minimum constellation for an active Na(+)-/K(+) ATPase is one alpha (α) and one beta (β) subunit. Mammals express four α isoforms (α1-4), encoded by the ATP1A1-4 genes, respectively. The α1 isoform is ubiquitously expressed in the adult central nervous system (CNS) whereas α2 primarily is expressed in astrocytes and α3 in neurons. Na(+) and K(+) are the principal ions involved in action potential propagation during neuronal depolarization. The α1 and α3 Na(+)-/K(+) ATPases are therefore prime candidates for restoring neuronal membrane potential after depolarization and for maintaining neuronal excitability. The α3 isoform has approximately four-fold lower Na(+) affinity compared to α1 and is specifically required for rapid restoration of large transient increases in [Na(+)]i. Conditions associated with α3 deficiency are therefore likely aggravated by suprathreshold neuronal activity. The α3 isoform been suggested to support re-uptake of neurotransmitters. These processes are required for normal brain activity, and in fact autosomal dominant de novo mutations in ATP1A3 encoding the α3 isoform has been found to cause the three neurological diseases Rapid Onset Dystonia Parkinsonism (RDP), Alternating Hemiplegia of Childhood (AHC), and Cerebellar ataxia, areflexia, pes cavus, optic atrophy, and sensorineural hearing loss (CAPOS). All three diseases cause acute onset of neurological symptoms, but the predominant neurological manifestations differ with particularly early onset of hemiplegic/dystonic episodes and mental decline in AHC, ataxic encephalopathy and impairment of vision and hearing in CAPOS syndrome and late onset of dystonia/parkinsonism in RDP. Several mouse models have been generated to study the in vivo consequences of Atp1a3 modulation. The different mice show varying degrees of hyperactivity, gait problems, and learning disability as well as stress-induced seizures. With the advent of several Atp1a3-gene or chemically modified animal models that closely phenocopy many aspects of the human disorders, we will be able to reach a much better understanding of the etiology of RDP, AHC, and CAPOS syndrome.
A sublimation apparatus for rapid separations (1954)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA sublimation apparatus for rapid separationsLewis N. Pino and Winfield S. Zehrung Cite this: J. Chem. Educ. 1954, 31, 9, 476Publication Date (Print):September 1, 1954Publication History Received3 August 2009Published online1 September 1954Published inissue 1 September 1954https://doi.org/10.1021/ed031p476RIGHTS & PERMISSIONSArticle Views111Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (571 KB) Get e-Alerts Get e-Alerts
Ray interpretation of the material signature in the acoustic microscope (1979)

The output voltage of the reflection acoustic microscope depends on the location on the object surface in a way that is characteristic of its elastic properties. We present a ray model showing that this dependence is due to interference between a narrow bundle of axial rays and rays associated with the leaky Rayleigh wave excited on the surface.
A Novel Strategy on Smooth Connection of an Offline MMC Station Into MTDC Systems (2015)

Themodular multilevel converter-based multiterminal HVDC (MMC-MTDC) technology has been applied to the Nanao project, which was commissioned successfully in China, and has shown many advantages. However, how to connect an offline MMC station into an operating MTDC system smoothly without expensive dc circuit breakers presents a huge challenge. Making full use of the structural characteristics of MMC, the proposed strategy implements the smooth connection by sequential operations of the dc disconnectors (DSs) at the positive and negative polarities, without the need for changing system parameters or hardware. The charging circuits of MMC are studied fully after every operation of DSs, and a four-order state equation is built to explore the potential maximum surge current after closing the DSs. Case study and simulation are performed based on the PSCAD/EMTDC model of the Nanao three-terminal MMC-HVDC system to validate the effectiveness of the proposed strategy. Besides easy operation and high reliability, the strategy neither influences the operating system nor increases any cost.
Finite-difference time-domain model of lasing action in a four-level two-electron atomic system: erratum (2006)

An erratum is presented to acknowledge a reference omitted from the original paper.
Induction of therapeutic levels of HbF in genome‐edited primary β<sup>0</sup>39‐thalassaemia haematopoietic stem and progenitor cells (2020)

Hereditary persistence of fetal haemoglobin (HPFH) is the major modifier of the clinical severity of β-thalassaemia. The homozygous mutation c.-196 C>T in the Aγ-globin (HBG1) promoter, which causes Sardinian δβ<sup>0</sup> -thalassaemia, is able to completely rescue the β-major thalassaemia phenotype caused by the β<sup>0</sup> 39-thalassaemia mutation, ensuring high levels of fetal haemoglobin synthesis during adulthood. Here, we describe a CRISPR/Cas9 genome-editing approach, combined with the non-homologous end joining (NHEJ) pathway repair, aimed at reproducing the effects of this naturally occurring HPFH mutation in both HBG promoters. After selecting the most efficient guide RNA in K562 cells, we edited the HBG promoters in human umbilical cord blood-derived erythroid progenitor 2 cells (HUDEP-2) and in haematopoietic stem and progenitor cells (HSPCs) from β<sup>0</sup> -thalassaemia patients to assess the therapeutic potential of HbF induction. Our results indicate that small deletions targeting the -196-promoter region restore high levels of fetal haemoglobin (HbF) synthesis in all cell types tested. In pools of HSPCs derived from homozygous β<sup>0</sup> 39-thalassaemia patients, a 20% editing determined a parallel 20% increase of HbF compared to unedited pools. These results suggest that editing the region of HBG promoters around the -196 position has the potential to induce therapeutic levels of HbF in patients with most types of β-thalassaemia irrespective of the β-globin gene (HBB) mutations.
Calcium chloride hexahydrate: a phase-changing material for energy storage (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCalcium chloride hexahydrate: a phase-changing material for energy storageHans Feilchenfeld and Sara SarigCite this: Ind. Eng. Chem. Prod. Res. Dev. 1985, 24, 1, 130–133Publication Date (Print):March 1, 1985Publication History Published online1 May 2002Published inissue 1 March 1985https://pubs.acs.org/doi/10.1021/i300017a024https://doi.org/10.1021/i300017a024research-articleACS PublicationsRequest reuse permissionsArticle Views1171Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Resident Microbiota Affect Bordetella pertussis Infectious Dose and Host Specificity (2013)

Before contacting host tissues, invading pathogens directly or indirectly interact with host microbiota, but the effects of such interactions on the initial stages of infection are poorly understood. Bordetella pertussis is highly infectious among humans but requires large doses to colonize rodents, unlike a closely related zoonotic pathogen, Bordetella bronchiseptica, raising important questions about the contributions of bacterial competition to initial colonization and host selection. We observed that <100 colony-forming units (CFU) of B. bronchiseptica efficiently infected mice and displaced culturable host microbiota, whereas 10 000 CFU of B. pertussis were required to colonize murine nasal cavities and did not displace host microorganisms. Bacteria isolated from murine nasal cavities but not those from the human lower respiratory tract limited B. pertussis growth in vitro, indicating that interspecies competition may limit B. pertussis colonization of mice. Further, a broad-spectrum antibiotic treatment delivered before B. pertussis inoculation reduced the infectious dose to <100 CFU, and reintroduction of single Staphylococcus or Klebsiella species was sufficient to inhibit B. pertussis colonization of antibiotic-treated mice. Together, these results reveal that resident microorganisms can prevent B. pertussis colonization and influence host specificity, and they provide rationale for manipulating microbiomes to create more-accurate animal models of infectious diseases.
Synthetic and Structural Studies of Titanium Aminotroponiminate Complexes (1999)

The synthesis and characterization of a series of Ti(III) and Ti(IV) aminotroponiminate complexes are described. Six-coordinate [TiMe(2)(Me(2)ATI)(2)] and [TiPh(2)(Me(2)ATI)(2)] were synthesized and structurally characterized, where Me(2)ATI is N,N'-dimethylaminotroponiminate. The mono-alkyl complexes [TiClR(Me(2)ATI)(2)], R = Me, CH(2)SiMe(3), were prepared, and treatment of the former, generated in situ, with PhMgCl yielded the alkyl-aryl complex [TiMePh(Me(2)ATI)(2)]. The solid state structures of most of these complexes were determined and reveal slightly distorted, trigonal-prismatic coordination geometries. Attempts to prepare alkyl complexes containing beta-hydrogen atoms resulted instead in the isolation of [Ti(Me(2)ATI)(3)] or [Ti(2)Cl(2)(Me(2)ATI)(4)], depending on the alkyl reagent and stoichiometry. Because of the modest steric requirements of the {Ti(Me(2)ATI)(2)}(2+) fragment, five-coordinate Ti(IV) complexes were only generated with a bulky sigma-2pi donor ligand, [Ti(N-(2,6)-i-Pr(2)C(6)H(3))(Me(2)ATI)(2)] being a specific example. Attempts to prepare the isoelectronic oxo analogue afforded only dimeric [Ti(2)O(2)(Me(2)ATI)(4)]. Comparison of the metrical parameters for these complexes with those in the literature containing aryloxide and benzamidinate ligands indicate that the {Ti(Me(2)ATI)(2)}(2+) fragment is quite electron releasing.
A Literatura Infantil como oportunidade de abordagem aos valores na Educação Pré-Escolar (2020)

A globalização fez emergir um conjunto de paradigmas sociais, económicos e culturais que alteraram por completo o mundo que costumávamos conhecer. A realidade contemporânea é verdadeiramente complexa, mas impôs-se de uma forma demasiado rápida e até agressiva, o que nos impede de a compreender de forma ampla. Neste contexto, os valores que costumavam defi nir-nos enquanto indivíduos e enquanto cidadãos vêm perdendo a sua nitidez, gerando uma dificuldade crescente em percebermos como orientar as nossas condutas. Perante esta perigoso panorama, urge a necessidade de a Escola assumir a sua responsabilidade fundamental de formar cidadãos esclarecidos, ativos e responsáveis. Só através de um processo de constante refl exão sobre si própria, sobre o outro e sobre os diversos modos de agir, em que o pensamento crítico seja sempre valorizado, a criança poderá desenvolver-se de forma plena, formando a sua identidade com clareza e aprendendo a lidar com a alteridade de forma harmoniosa. Particularmente no Jardim de Infância, a Literatura Infantil assume-se, pela sua riqueza estética e literária, mas também pela relação tão singular que se forma entre a criança e o livro, como um meio de excelência para a abordagem e a refl exão sobre os valores.
Structure–Activity Relationship Study of 2,4‐Dianilinopyrimidine Containing Methanesulfonamide (TRE‐069) as Potent and Selective Epidermal Growth Factor Receptor T790M/C797S Mutant Inhibitor for Anticancer Treatment (2017)

Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Investigation of broad resonances in <sup>31</sup>P NMR spectra of the human brain <i>in vivo</i> (1994)

Broad resonances that lie underneath the familiar small molecule profile of in vivo 31P NMR spectra can make accurate spectral integration of these mobile phosphates difficult. The two major broad components are the phosphate contained in the hydroxyapatite in cranial bone and the phosphodiester moiety in partially mobile membrane phospholipids. They can be removed with post-acquisition processing but this results in distortion of lineshapes and intensities and interferes with accurate quantitation. We have employed an off-resonance saturation procedure to eliminate the bone resonance and isolate the signal from the membrane phospholipids by subtraction. Selective saturation of the phospholipid resonance increases the clarity of the sharp peaks downfield from the phosphocreatine peak. Selective saturation 3-D chemical shift imaging techniques were used to create a localized phospholipid profile of the entire brain simultaneously. Monitoring localized phospholipid concentration may be important in studying demyelinating diseases.
A sound-based approach to video indexing and its application (1998)

We have been carrying out research on intelligent video interfaces to improve the manipulation of videos for searching, editing, processing and to support rapid review of the contents using the characteristics of the videos. The soundtracks of the videos contain much information related to the events occurring in the scenes and the situations of the scenes. They also reflect the director's intentions expressed through the emotions of the cast. We can often estimate these events directly from the physical characteristics of the sound. Sound can be classified into three major categories: music, speech, and sound effects. In this paper, we propose algorithms for independently detecting music and speech, even if they occur together. When music is present, the spectrum peaks of the sound data appear to be stable in the frequency direction for a certain period of time. Music is detected by obtaining the positions of the peaks and their duration using an edge operator. After the removal of musical components, speech is detected by using a comb filter that is sensitive to the harmonic structure of vocal sound. Further, we present an example of an application interface that uses detected music and speech as indexes for browsing, accessing, and surveying video contents and discuss its effectiveness. © 1998 Scripta Technica, Syst Comp Jpn, 29(12): 1–10, 1998
Prevalence of Symptoms of Bladder Pain Syndrome/Interstitial Cystitis Among Adult Females in the United States (2011)

Bladder pain syndrome/interstitial cystitis symptoms are widespread among United States women and associated with considerable disability. These results suggest bladder pain syndrome/interstitial cystitis may be underdiagnosed.
Concerning the redistribution of beryllium alkyls and aryls with beryllium halides in diethyl ether (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConcerning the redistribution of beryllium alkyls and aryls with beryllium halides in diethyl etherJames R. Sanders Jr., E. C. Ashby, and James H. Carter IICite this: J. Am. Chem. Soc. 1968, 90, 23, 6385–6390Publication Date (Print):November 1, 1968Publication History Published online1 May 2002Published inissue 1 November 1968https://pubs.acs.org/doi/10.1021/ja01025a024https://doi.org/10.1021/ja01025a024research-articleACS PublicationsRequest reuse permissionsArticle Views85Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Olfaction and Parkinson's syndromes: its role in differential diagnosis (2004)

Research findings have confirmed a role for olfactory testing in the differential diagnosis of movement disorders, and suggest that this approach is currently underused in clinical practice. Validated test batteries are now available that may prove to be of practical use in the differential diagnosis of Parkinsonian syndromes and indeterminate tremors.