gebhart/patrepeval huggingface dataset:
Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor (2004)

Aggregations of 27 nm virus-like particles were observed in electron microscopy images of sectioned Varroa destructor mite tissue. The scattered occurrence of individual particles and accumulation of the virions in lattices in the cytoplasm gave an apparent indication that the virus replicates in the mite. Sequence analysis of the RNA of the purified virus revealed a genome organization with high similarity to that of members of the genus Iflavirus. Phylogenetic analysis of the polymerase showed that the virus was related most closely to Deformed wing virus (DWV) and Kakugo virus (KV) of bees. The virus has a genome of 10 112 nt without the poly(A) tail, with an overall RNA genome identity of 84 % to those of DWV and KV and has one large ORF, translated into a 2893 aa polyprotein with an amino acid identity of 95 % to those of DWV and KV. The first 1455 nt of the ORF encoding the lower molecular mass structural proteins shows the greatest diversion from those of DWV and KV, with an RNA identity of 79 %, and translates to a polypeptide of 485 aa with an identity of 90 %. The name proposed for this virus is Varroa destructor virus 1 (VDV-1). To determine whether VDV-1 replicates in mites, a selective RT-PCR was done to detect the presence of the negative-sense RNA strand. The virus isolate and the closely related DWV could be discriminated by two primer sets, each specific to one virus. Both viruses replicated in the population of the mite species studied.
Sodium-ion batteries: present and future (2017)

Energy production and storage technologies have attracted a great deal of attention for day-to-day applications. In recent decades, advances in lithium-ion battery (LIB) technology have improved living conditions around the globe. LIBs are used in most mobile electronic devices as well as in zero-emission electronic vehicles. However, there are increasing concerns regarding load leveling of renewable energy sources and the smart grid as well as the sustainability of lithium sources due to their limited availability and consequent expected price increase. Therefore, whether LIBs alone can satisfy the rising demand for small- and/or mid-to-large-format energy storage applications remains unclear. To mitigate these issues, recent research has focused on alternative energy storage systems. Sodium-ion batteries (SIBs) are considered as the best candidate power sources because sodium is widely available and exhibits similar chemistry to that of LIBs; therefore, SIBs are promising next-generation alternatives. Recently, sodiated layer transition metal oxides, phosphates and organic compounds have been introduced as cathode materials for SIBs. Simultaneously, recent developments have been facilitated by the use of select carbonaceous materials, transition metal oxides (or sulfides), and intermetallic and organic compounds as anodes for SIBs. Apart from electrode materials, suitable electrolytes, additives, and binders are equally important for the development of practical SIBs. Despite developments in electrode materials and other components, there remain several challenges, including cell design and electrode balancing, in the application of sodium ion cells. In this article, we summarize and discuss current research on materials and propose future directions for SIBs. This will provide important insights into scientific and practical issues in the development of SIBs.
Ionische Flüssigkeiten für die Synthese funktioneller Nanopartikel und anderer anorganischer Nanostrukturen (2004)

Abstract Ionische Flüssigkeiten sind eine neue Klasse organischer Lösungsmittel mit hoher Polarität und einer vororganisierten Lösungsmittelstruktur. In diesen Flüssigkeiten können in Abwesenheit oder bei einem kontrollierten Anteil von Wasser sehr polare Reaktionen durchgeführt werden, die auf einfache Weise und bei moderaten Temperaturen zu kristallinen Nanopartikeln führen. Die ausgeprägte Organisation dieser Lösungsmittel kann für die Herstellung selbstanordnender, hoch organisierter Hybrid‐Nanostrukturen von bisher nicht bekannter Qualität genutzt werden. Das außergewöhnliche Potenzial ionischer Flüssigkeiten für die Materialsynthese soll in diesem Kurzaufsatz von einem physikalisch‐chemischen Standpunkt aus dargelegt werden.
Differentiation of oligodendrocyte occurs in contact with astrocyte (1998)

We have generated stage-specific oligodendrocytes, which are identified as immature oligodendrocytes by the expression of specific markers. These cells were generated by using 3 types of intrinsic and extrinsic factors for influencing the characteristics of oligodendrocyte-lineage cells. The factors included 10% serum in the medium to induce cell proliferation, bFGF in serum-free medium to increase survival and induce differentiation, and 0.25% trypsin to remove nonoligodendrocyte lineage cells. We have also demonstrated that it is possible to supply a huge amount of homogeneous populations of stage-specific immature oligodendrocytes after 35 days' culture. The maturation of these cells is controlled by interacting with astrocytes in vitro, resulting in the expression of MBP isoforms (14.0 and 18.5 kDa) that may participate in mature oligodendrocyte membrane compaction.
Si / SiO2 Interface Oxidation Kinetics: A Physical Model for the Influence of High Substrate Doping Levels: II . Comparison with Experiment and Discussion (1979)

A physical model based on the statistics of silicon point defects was described in the preceding companion paper to explain the faster oxidation rates of heavily doped silicon. The mechanism by which dopant levels affect the interface oxidation rate was postulated to be an increase in reaction sites or silicon vacancies caused by the shifting of the Fermi level. The predictions of this model for n+ and p+ silicon oxidation kinetics are tested in this paper under a wide variety of oxidation conditions. The physical meaning of the model and its implications for other process phenomena are pursued.
Metals on Graphene: Interactions, Growth Morphology, and Thermal Stability (2013)

Graphene, a single atomic layer of graphite, has been a material of recent intensive studies due to its novel electronic and structural properties and its potential applications in the emerging area of carbon-based electronic devices. Metal on graphene growth is one of the current research interests, aiming at improving and manipulating the electronic and magnetic properties of graphene through metal atom adsorption or doping to meet various requirements in device applications. In this paper, we will give an overview of recent experimental and computational investigation of interaction, growth morphology, and thermal stability of various metals on graphene grown on 6H-SiC(0001) substrate.
The efficacy of glue traps against wild populations of house mice, Mus domesticus Rutty (1998)

Field research was conducted from Purdue University during 1991 to 1993 to examine some aspects of the efficaciousness of the various types of glue traps against wild populations of house mice. The research was conducted in agricultural and livestock buildings containing various infestation levels of mice. Tests compared the capture and escape rates of glue boards vs. trays, covered vs. uncovered glue traps, and glue traps vs. snap traps, and multiple catch curiosity traps. Observational work, via night vigils, was also conducted to note the behavioral response of mice to glue surfaces, including the behavioral aspects of mice neutralizing glue surfaces in well-used runways. These field tests indicate many mice, upon initial interactions with glue traps and surfaces, are repelled by them and either learn to avoid them or neutralized them in some manner. Results of comparison trials between glue traps and non-glue mouse traps also indicate strong differences in interaction and capture rates favoring non-glue traps. It is hypothesized that when glue traps are successful, it is likely due to mice traveling kinesthetically along frequently used runways in which traps are placed, or to factors associated with age class of mice. These studies have strong implications for rodent pest management programs in facilities which are restricted to non-chemical approaches (e.g., food handling establishments and sensitive accounts).
Recent developments in bulk and thin film PLZT materials and devices (1992)

Abstract Current trends in the electronics and optoelectronics industries involving computing, data processing, sensing and communications all point toward a continuing need for improved materials and new multifunctional structures. The continuing and unrelenting drive toward higher bit density and greater integration has led many of the new technologies away from bulk materials and to their thin film counterparts. Recent research on thin and thin/thick PLZT for ferroelectric and electrooptic applications has also participated in this trend toward thinner structures. Their desirable ferroelectric switching properties have been explored for random access memory applications, whereas, their electrooptic properties have been evaluated for transverse-mode light shutters and modulators. Shutter contrast ratios as high as 500:1 have been obtained.
Characterization of putative Haemophilus somnus isolates from tonsils of American bison (Bison bison). (1999)

Three Haemophilus somnus isolates (2a, 3a, and 27b) and one H. somnus-like (13b) isolate from tonsils of commercially reared American bison were compared with 2 known H. somnus isolates from cattle, namely, 2336, shown to cause respiratory disease, and 129Pt, from the prepuce of an asymptomatic bull. All H. somnus isolates, but not the H. somnus-like isolate, required CO2 for growth. Biochemical utilization profiles were identical for bison and bovine H. somnus isolates with the exception of alpha-fucosidase production by isolate 3a. Isolate 27a varied from 2a, 2336 and 129Pt by hemolysis of bovine erythrocytes. Isolate 13b hemolyzed sheep but not bovine or bison erythrocytes and varied from other isolates in biochemical utilization tests. Outer membrane protein profiles of 2a, 3a and 27a were almost identical with those of bovine isolate 2336 and similar to that of 129Pt, but quite different from that of 13b. Western blots of bison isolates were similar to that of the virulent bovine 2336 isolate, including detection of high molecular mass antigens above 100 kDa and the 76 kDa antigens associated with bovine IgG2 Fc binding characteristic of virulent strains, as well as antigens of approximately 78, 60 and 40 kDa. Producers and veterinarians should be aware that H. somnus may be carried by bison and may have potential for causing diseases in bison similar to those described in cattle and sheep.
HMGB1 induces angiogenesis in rheumatoid arthritis via HIF‐1α activation (2014)

High-mobility group box protein 1 (HMGB1), a nonhistone nuclear protein and a cytokine mediator, is implicated in the pathogenesis of rheumatoid arthritis (RA). Extracellular HMGB1 binds to its receptors and triggers downstream signal cascade leading to the perpetuation of synovitis and local tissue invasion. Here, we investigated a novel role of HMGB1 in regulating hypoxia-inducible factor (HIF)-1α to mediate angiogenesis in RA synovium. HIF-1α mRNA levels and activities in synovial fibroblasts from RA patients were enhanced by HMGB1. Pharmacological inhibition of TLR4 and NF-kappaB activation blocked the HMGB1-dependent upregulation of HIF-1α mRNA expression and its activity, suggesting the involvement of transcriptional regulation. HMGB1 stimulated expression of vascular endothelial growth factor (VEGF), and inhibition of HIF-1α attenuated HMGB1-induced VEGF. Conditioned media derived from HMGB1-stimulated synovial fibroblasts enhanced tube formation in human microvascular endothelial cells by upregulating HIF-1α. In the joint tissues of mice with collagen-induced arthritis, treatment with anti-HMGB1 neutralizing antibody prevented blood vessel formation in association with decreased expression of HIF-1α. These observations support the idea that increased HMGB1 induces an extension of inflamed synovium by accelerating angiogenesis in RA through enhancement of HIF-1α activation. Therefore, inhibition of HMGB1 could prove beneficial for the treatment of angiogenesis in RA.
The Chaperone Balance Hypothesis: The Importance of the Extracellular to Intracellular HSP70 Ratio to Inflammation‐Driven Type 2 Diabetes, the Effect of Exercise, and the Implications for Clinical Management (2015)

Recent evidence shows divergence between the concentrations of extracellular 70 kDa heat shock protein [eHSP70] and its intracellular concentrations [iHSP70] in people with type 2 diabetes (T2DM). A vital aspect regarding HSP70 physiology is its versatility to induce antagonistic actions, depending on the location of the protein. For example, iHSP70 exerts a powerful anti-inflammatory effect, while eHSP70 activates proinflammatory pathways. Increased eHSP70 is associated with inflammatory and oxidative stress conditions, whereas decreased iHSP70 levels are related to insulin resistance in skeletal muscle. Serum eHSP70 concentrations are positively correlated with markers of inflammation, such as C-reactive protein, monocyte count, and TNF-α, while strategies to enhance iHSP70 (e.g., heat treatment, chemical HSP70 inducers or coinducers, and physical exercise) are capable of reducing the inflammatory profile and the insulin resistance state. Here, we present recent findings suggesting that imbalances in the HSP70 status, described by the [eHSP70]/[iHSP70] ratio, may be determinant to trigger a chronic proinflammatory state that leads to insulin resistance and T2DM development. This led us to hypothesize that changes in this ratio value could be used as a biomarker for the management of the inflammatory response in insulin resistance and diabetes.
cAMP Analog Mapping of Epac1 and cAMP Kinase (2003)

Little is known about the relative role of cAMP-dependent protein kinase (cAPK) and guanine exchange factor directly activated by cAMP (Epac) as mediators of cAMP action. We tested cAMP analogs for ability to selectively activate Epac1 or cAPK and discriminate between the binding sites of Epac and of cAPKI and cAPKII. We found that commonly used cAMP analogs, like 8-Br-cAMP and 8-pCPT-cAMP, activate Epac and cAPK equally as well as cAMP, i.e. were full agonists. In contrast, 6-modified cAMP analogs, like N6-benzoyl-cAMP, were inefficient Epac activators and full cAPK activators. Analogs modified in the 2'-position of the ribose induced stronger Epac1 activation than cAMP but were only partial agonists for cAPK. 2'-O-Alkyl substitution of cAMP improved Epac/cAPK binding selectivity 10-100-fold. Phenylthio substituents in position 8, particularly with MeO- or Cl- in p-position, enhanced the Epac/cAPK selectivity even more. The combination of 8-pCPT- and 2'-O-methyl substitutions improved the Epac/cAPK binding selectivity about three orders of magnitude. The cAPK selectivity of 6-substituted cAMP analogs, the preferential inhibition of cAPK by moderate concentrations of Rp-cAMPS analogs, and the Epac selectivity of 8-pCPT-2'-O-methyl-cAMP was also demonstrated in intact cells. Using these compounds to selectively modulate Epac and cAPK in PC-12 cells, we observed that analogs selectively activating Epac synergized strongly with cAPK specific analogs to induce neurite outgrowth. We therefore conclude that cAMP-induced neurite outgrowth is mediated by both Epac and cAPK.
Variation-Tolerant, Power-Safe Pattern Generation (2007)

By generating safe patterns - those that tolerate on-chip variations - this framework avoids false delay test failures. It uses power grid information and regional constraints on switching activity to minimize peak power and optimize the pattern set. Experimental results on benchmark circuits demonstrate the framework's effectiveness.
Concentrations of acidic antiinflammatory drugs in gastric mucosa. (1977)

In rats, the concentrations of the acidic antiinflammatory drugs salicylic acid, acetylsalicylic acid, phenylbutazone, flufenamic acid and indomethacin in the glandular portion of the gastric mucosa were determined 30 and 60 min after oral or subcutaneous administration. In another series of experiments, solutions of the drugs were introduced into the ligated stomach and the concentrations in the mucosa and in the contents of the stomach were determined after 60 min. The ratio between the concentrations in the musoca and those in serum or gastric contents were much lower than expected according to the distribution by passive non-ionic diffusion. This apparent discrepancy may be explained as a result of a drug-induced damage to the mucosal cell allowing free diffusion of ionized drug across the cell membrane.
Syrosingopine sensitizes cancer cells to killing by metformin (2016)

We report that the anticancer activity of the widely used diabetic drug metformin is strongly potentiated by syrosingopine. Synthetic lethality elicited by combining the two drugs is synergistic and specific to transformed cells. This effect is unrelated to syrosingopine's known role as an inhibitor of the vesicular monoamine transporters. Syrosingopine binds to the glycolytic enzyme α-enolase in vitro, and the expression of the γ-enolase isoform correlates with nonresponsiveness to the drug combination. Syrosingopine sensitized cancer cells to metformin and its more potent derivative phenformin far below the individual toxic threshold of each compound. Thus, combining syrosingopine and codrugs is a promising therapeutic strategy for clinical application for the treatment of cancer.
Dynamic Spectrum Access: Signal Processing, Networking, and Regulatory Policy (2006)

In this article, we first provide a taxonomy of dynamic spectrum access. We then focus on opportunistic spectrum access, the overlay approach under the hierarchical access model of dynamic spectrum access. we aim to provide an overview of challenges and recent developments in both technological and regulatory aspects of opportunistic spectrum access.
<title>Computing local and global similarity in images</title> (1998)

The retrieval of images based on their visual similarity to an example image is an important and fascinating area of research. Here, we discuss various ways in which visual appearance may be characterized for determining both global and local similarity in images. One popular method involves the computation of global measures like moment invariants to characterize global similarity. Although this means that the image may be characterized using a few numbers, the performance is often poor. Techniques based on moment invariants often perform poorly. They require that the object be a binary shape without holes which is often not practical. In addition, moment invariants are sensitive to noise. Visual appearance is better represented using local features computed at multiple scales. Such local features may include the outputs of images filtered with Gaussian derivatives, differential invariants or geometric quantities like curvature and phase. Two images may be said to be similar if they have similar distributions of such features. Global similarity may, therefore, be deduced by comparing histograms of such features. This can be done rapidly. Histograms cannot be used to compute local similarity. Instead, the constraint that the spatial relationship between the features in the query be similar to the spatial relationship between the features of its matching counterparts in the database provides a means for computing local similarity. The methods presented here do not require prior segmentation of the database. In the case of local representation objects can be embedded in arbitrary backgrounds and both methods handle a range of size variations and viewpoint variations up to 20 or 25 degrees.
Mechanical Cell Injury (2006)

The tissues of the body are continually subjected to mechanical stimulation by external forces, such as gravity, and internally generated forces, such as the pumping of blood or muscle contraction. Within a physiological range, the forces elicit adaptive responses acutely (to rapidly alter function) and chronically (to remodel tissue structure to optimize load-bearing capabilities). When the forces exceed certain thresholds, injury results. To understand the mechanisms of mechanical injury at the cellular level, we must analyze the structural response of the cell to various modes of deformation and examine the biological consequences of the structural alterations caused by the trauma. This chapter reviews the mechanics of cell membrane deformation and failure. Evidence for the strain-rate-dependent, transient disruption of cell membranes, or mechanoporation, is presented for a variety of cell types. The complex interactions between the structural damage and the biological sequelae are illustrated using clinically relevant forms of cell injury. Finally, novel therapeutic approaches targeting membrane integrity are described.
Polyploid formation created unique avenues for response to selection in <i>Gossypium</i> (cotton) (1998)

A detailed restriction fragment length polymorphism map was used to determine the chromosomal locations and subgenomic distributions of quantitative trait loci (QTLs) segregating in a cross between cultivars of allotetraploid (AADD) Gossypium hirsutum ("Upland" cotton) and Gossypium barbadense ("Sea Island," "Pima," or "Egyptian" cotton) that differ markedly in the quality and quantity of seed epidermal fibers. Most QTLs influencing fiber quality and yield are located on the "D" subgenome, derived from an ancestor that does not produce spinnable fibers. D subgenome QTLs may partly account for the fact that domestication and breeding of tetraploid cottons has resulted in fiber yield and quality levels superior to those achieved by parallel improvement of "A" genome diploid cottons. The merger of two genomes with different evolutionary histories in a common nucleus appears to offer unique avenues for phenotypic response to selection. This may partly compensate for reduction in quantitative variation associated with polyploid formation and be one basis for the prominence of polyploids among extant angiosperms. These findings impel molecular dissection of the roles of divergent subgenomes in quantitative inheritance in many other polyploids and further exploration of both "synthetic" polyploids and exotic diploid genotypes for agriculturally useful variation.
유아교사의 5세 누리과정 교육연수 참여 실태 및 요구분석 (2013)

본 연구는 유아교사의 배경변인에 따른 5세 누리과정⌟의 교육연수 참여 실태와 요구를 파악 해 봄으로써 『누리과정』의 효율적인 현장적용 위한 교사연수, 교사 지원체제 개발 등의 기초자료를 제공하는 데 목적이 있다. 5세 누리과정 교육연수 참여 실태 분석은 누리과정 교육연수 참여경험 유무, 주관기관, 참여 도움정도, 추후 교육연수 참여의사 및...
A Study of the Effects of Sucrose Concentration, Lacquer Concentration and Coating Time on the Formulation of Stable and Effective Carbenicillin Indanyl Sodium Microcapsules (1988)

AbstractCarbenicillin indanyl sodium, commonly known as Geocillin (GC), is an orally effective derivative of carbenicillin employed in the treatment of gram negative infections of the urinary tract. GC exhibits an extremely bitter taste which affects patient compliance upon oral dosing (1). A novel coating approach allows Geocillin to be prepared as a suspension for oral administration. GC is available only as a tablet.Eudragit E100R [EE] is a tasteless, acid soluble cationic polymer. Encapsulation of GC with [EE] inhibited its release in the mouth, thus overcoming its bitter taste. Dissolution studies were carried out in simulated gastric fluid and simulated intestinal fluid. Three factors, viz. sucrose concentration, lacquer concentration and coating time were evaluated to arrive at an optimally acceptable formulation.The formulation containing GC and sucrose in the ratio of 1:3, suspension coated using a 5% w/w lacquer solution for 40 mins. yielded taste free microcapsules with optimal release characteristics.