gebhart/patrepeval huggingface dataset:
Electrochemical immunosensor modified with self-assembled monolayer of 11-mercaptoundecanoic acid on gold electrodes for detection of benzo[a]pyrene in water (2012)

Well-oriented bio-conjugates on gold electrode surfaces will indirectly influence the molecular recognition of antigens to surface bound antibodies thus improving the detection performance of electrochemical immunosensors. This paper describes the modification of self-assembled monolayers (SAMs) on gold electrode surface with 11-mercaptoundecanoic acid (11-MUA). Activation of carboxylic acid terminal was performed by reaction of a mixture of water soluble carbodiimide and N-hydrosuccinimide (NHS) on the electrode surfaces. Characterisation of the SAM formation on the gold electrode was performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and contact angle measurements. An amperometric immunosensor was developed for the screening of polycyclic aromatic hydrocarbons (PAHs) in water. The system consists of gold as the working electrode, platinum as the counter electrode and a Ag/AgCl reference electrode. This three electrode system is integrated on a single chip. The measurement employs the enzyme-linked immunosorbent assay (ELISA) principle. Benzo[a]pyrene (BaP) was detected using an immunological reaction by measuring the alkaline phosphatase (AP) enzymatic reaction towards the substrate para-amino phenyl phosphate (pAPP). A competitive assay was performed within the electrode using AP as the labelled-enzyme. A lower limit of detection (5.6 ng ml(-1)) of BaP was achieved after the activation of the mixture of carbodiimide and succinimide with the alkanethiol SAM on the gold electrode in comparison to that obtained for the unmodified electrode (14.2 ng ml(-1)). The developed surface functionalised sensor demonstrated acceptable reproducibility and good stability, with a wide linear response to BaP (4-140 ng ml(-1)).
Fine-Pruning: Joint Fine-Tuning and Compression of a Convolutional Network with Bayesian Optimization (2017)

When approaching a novel visual recognition problem in a specialized image domain, a common strategy is to start with a pre-trained deep neural network and fine-tune it to the specialized domain. If the target domain covers a smaller visual space than the source domain used for pre-training (e.g. ImageNet), the fine-tuned network is likely to be over-parameterized. However, applying network pruning as a post-processing step to reduce the memory requirements has drawbacks: fine-tuning and pruning are performed independently; pruning parameters are set once and cannot adapt over time; and the highly parameterized nature of state-of-the-art pruning methods make it prohibitive to manually search the pruning parameter space for deep networks, leading to coarse approximations. We propose a principled method for jointly fine-tuning and compressing a pre-trained convolutional network that overcomes these limitations. Experiments on two specialized image domains (remote sensing images and describable textures) demonstrate the validity of the proposed approach.
Comparison of clinical performances among Roche Cobas HPV, RFMP HPV PapilloTyper and Hybrid Capture 2 assays for detection of high-risk types of human papillomavirus (2015)

The cervical cancer screening guidelines suggest that early detection of HPV16 and HPV18 is helpful for identifying women with cervical intraepithelial neoplasia (CIN) grade two or higher. We comparatively evaluated three HPV DNA assays, Roche Cobas HPV, RFMP HPV PapilloTyper, and Hybrid Capture 2 (HC2). A total of 861 cervical swab samples from women over 30 years of age were classified into two groups, that is, high grade squamous intraepithelial lesion (HSIL) and non-HSIL, according to cervical cytology results and analyzed by three assays. The results of direct sequencing or Linear array HPV genotyping test were considered true when the three assays presented discrepancies. The concordance rates between Roche Cobas HPV versus RFMP HPV PapilloTyper, RFMP HPV PapilloTyper versus HC2, and Roche Cobas versus HC2 were 94.5%, 94.3%, and 95.9%, respectively. For detection of HPV16 and HPV18, Roche Cobas HPV showed the concordance rates of 98.3% (κ = 0.73) and 99.4% (κ = 0.40) with the confirmation tests, respectively; and RFMP HPV PapilloTyper showed the concordance rates of 99.5% (κ = 0.92) and 100.0% (κ = 1.00), respectively. In conclusion, Roche Cobas HPV, RFMP HPV PapilloTyper, and HC2 showed high agreement rates. Roche Cobas HPV and RFMP HPV PapilloTyper are particularly useful, since both provide HPV specific genotypes, HPV16 and HPV18. J. Med. Virol. 87:1587–1593, 2015. © 2015 Wiley Periodicals, Inc.
A distributed algorithm for detecting resource deadlocks in distributed systems (1982)

This paper presents a distributed algorithm to detect deadlocks in distributed data bases. Features of this paper are (1) a formal model of the problem is presented, (2) the correctness of the algorithm is proved, i.e. we show that all true deadlocks will be detected and deadlocks will not be reported falsely, (3) no assumptions are made other than that messages are received correctly and in order and (4) the algorithm is simple.
Application of probabilistic neural network and static test data to the classification of bridge damage patterns (2003)

This paper is primarily concerned with the applicaitonof static test data in conjunction withthe probabilistic neural nework (PNN) for the classificationof dmage patterns of a cable-stayed bridge. A total of 11 dmage patterns are considered by combinationof 5 typical dmage regions. Both training and testing data, derived from static analysis via finite element method (FEM), are contaminated with differnt noise level to simula eth eFe model and meausmeent erros. The study of damge pattern identificaitonis conducted by taking into account the change ratios of the deflection of the main beam and the towe runder loading as input neuron sof the pNN. The effects of noise levls, the types of damage patterns, and the number of input neurons on the identification accuracy ae investigated.Base don the classificaiton results some valuable conclusions were obtained.
<title>Software radio technology and applications to law enforcement</title> (1997)

Law enforcement use of radio includes the rapid creation of networks for the dozens of law enforcement organizations who come together in situations as diverse as the TWA 800 disaster in New York or the SunFest celebration in Palm Beach. The software radio is a proven technology for rapidly building such interoperable networks, including seamless bridging cross sub-networks of different frequency bands, channel modulations and information formats. In addition, law enforcement must manage the costs of related radio base station infrastructure, mobile units and handsets. The software radio is a collection of engineering techniques for creating radio infrastructure that can be programmed for new standards and that can be dynamically updated with new software personalities even 'over the air,' reducing the need to purchase new hardware to remain current with emerging radio interface standards. Although relatively expensive today, continuing DoD, federal and commercial investment in software radio technology will bring products within the reach of law enforcement applications within the next few years. The Modular Multifunction Information Transfer Systems (MMITS) Forum provides further impetus for cost reductions through the market efficiencies of open architecture. This article summarizes software radio technology and key trends in the marketplace including the progress of the MMITS forum. Expanded law enforcement participation in this forum would accelerate the availability of low cost products for law enforcement.
A New Way of Controlling Parallel-Connected Inverters by Using Synchronous-Reference-Frame Virtual Impedance Loop—Part I: Control Principle (2015)

A novel simple and effective autonomous current-sharing controller for parallel three-phase inverters is proposed in this paper. The proposed controller provides faster response and better accuracy in contrast to the conventional droop control, since this novel approach does not require any active or reactive power calculations. Instead, a synchronous-reference-frame (SRF) virtual impedance loop and an SRF-based phase-locked loop are used. Stationary analysis is provided in order to identify the inherent mechanism of the direct and quadrature output currents in relation to the voltage amplitude and frequency with different line impedances by means of the system transfer functions. Comparison experiments from two parallel inverters are presented to compare the control performance of the conventional droop control and the proposed control with different line impedances. In addition, experimental results from a setup with three parallel 2.2-kW inverters verify the effectiveness of the proposed control strategy in different scenarios.
Linkage intensity learning approach with genetic algorithm for causality diagram (2007)

诱发性图理论，采用知识和诱发性的直接紧张的图形的表示，在信仰网络克服一些缺乏并且为分离、连续的变量演变为混合诱发性图方法论。但是给连接诱发性图的紧张是困难的，特别地处于采样数据有限或吵闹的许多工作在条件。经典学习算法是难的被收养。我们使用了基因算法从有限数据学习连接紧张。模拟结果证明这个算法是比处于象航天飞机的差错 diagnoisis 那样的样品缺乏的条件的经典算法更合适的。
Targeted mutagenesis using zinc-finger nucleases in <i>Arabidopsis</i> (2005)

Targeted mutagenesis is an essential tool of reverse genetics that could be used experimentally to investigate basic plant biology or modify crop plants for improvement of important agricultural traits. Although targeted mutagenesis is routine in several model organisms including yeast and mouse, efficient and widely usable methods to generate targeted modifications in plant genes are not currently available. In this study we investigated the efficacy of a targeted-mutagenesis approach based on zinc-finger nucleases (ZFNs). In this procedure, ZFNs are used to generate double-strand breaks at specific genomic sites, and subsequent repair produces mutations at the break site. To determine whether ZFNs can cleave and induce mutations at specific sites within higher plant genomes, we introduced a construct carrying both a ZFN gene, driven by a heat-shock promoter, and its target into the Arabidopsis genome. Induction of ZFN expression by heat shock during seedling development resulted in mutations at the ZFN recognition sequence at frequencies as high as 0.2 mutations per target. Of 106 ZFN-induced mutations characterized, 83 (78%) were simple deletions of 1-52 bp (median of 4 bp), 14 (13%) were simple insertions of 1-4 bp, and 9 (8%) were deletions accompanied by insertions. In 10% of induced individuals, mutants were present in the subsequent generation, thus demonstrating efficient transmission of the ZFN-induced mutations. These data indicate that ZFNs can form the basis of a highly efficient method for targeted mutagenesis of plant genes.
Novel Modular DNA Aptamer for Human Thrombin with High Anticoagulant Activity (2011)

Aptamers based on nucleic acids are a promising alternative to antibodies in therapy and diagnostics. Several DNA aptamers against human thrombin have been developed by selection from random libraries: a 15-mer and its derivatives, a 29-mer, and a 31-mer. Some of them are patented and already under clinical trial. The 15-mer structure was determined by X-ray and NMR and turned out to be a monomolecular antiparallel G-quadruplex. The other aptamers mentioned above have higher inhibitory activity than the initial 15-mer, but there are not yet structural data explaining this phenomenon. Here, the initial 15-mer, 31-mer, and novel RA-36 aptamers are compared to establish the structure-function correlation, providing a solid ground for further rational aptameric drug design. For the molecular dynamic simulation of aptamers, the force field parmbsc0 was ported onto GROMACS, and the main stabilizing parameters were revealed, leading to the novel DNA aptamer RA-36. The functional properties of the DNA aptamers were studied by conventional coagulation tests, which do not directly elucidate the mechanism of thrombin inhibition by aptamers. Improved turbidimetric measurements provided data to develop detailed kinetics showing that the 31-mer and RA-36, in contrast to the 15-mer, are competitive inhibitors. These data revealed RA-36 to be an efficient thrombin inhibitor with a dose-dependent effect. Animal tests of the studied DNA aptamers suggested an unexpected species-specificity of the novel RA-36.
Medical Image Processing, Reconstruction and Restoration (2005)

It is essential that differently oriented specialists and students involved in image processing have a firm grasp of the necessary concepts and principles. A single-source reference that can provide this foundation, as well as a thorough explanation of the techniques involved, particularly those found in medical image processing, would be an
A 10-Gb/s optical asynchronous cell/packet receiver with a fast bit-synchronization circuit (2003)

A 10-Gbit/s optical asynchronous cell-packet receiver with a fast bit-synchronization circuit has been developed for achieving beyond Tbit/s optical switch. Less than nine-bit fast acquisition time and <1.5 dB receiver sensitivity penalty have been achieved.
Silicon micro-ring resonator device design for optical interconnect systems (2013)

Optical interconnect systems is one of the most promising solutions to surpass the speed bottleneck of CPU from the electrical interconnects. To realize this idea, one of the key topics is to design compact photonic devices suitable for building the optical interconnects. ? The use of silicon microring resonators is an attractive technology for the integrated photonics pursuing optical interconnect applications, highly exciting due to the high integration density, low power consumption, and versatile functionalities. The platform technologies available for silicon microring resonators can be categorized by their structures, functions in optical interconnect systems, etc. Specific designs are in great needed to break off some critical tradeoffs in these silicon microring-based devices performances. ? In this dissertation, the design and simulations of silicon microring based photonic devices for optical interconnects are presented. Utilizing the proposed technologies, various types of optical elements such as resonance-switching/coupling-switching OOK modulators, DPSK modulators and filters are designed. The systematic performance is studied as well. The data have shown that, by employing our unique designs critical tradeoffs in the device performance can be successfully broken and great data quality at ultra-high data transmission speed can be obtained.
Oxygen Vacancies Lead to Loss of Domain Order, Particle Fracture, and Rapid Capacity Fade in Lithium Manganospinel (LiMn<sub>2</sub>O<sub>4</sub>) Batteries (2014)

Spinel-structured lithium manganese oxide (LiMn2O4) has attracted much attention because of its high energy density, low cost, and environmental impact. In this article, structural analysis methods such as powder neutron diffraction (PND), X-ray diffraction (XRD), and high-resolution transmission and scanning electron microscopies (TEM & SEM) reveal the capacity fading mechanism of LiMn2O4 as it relates to the mechanical degradation of the material. Micro-fractures form after the first charge (to 4.45 V vs. Li(+/0)) of a commercial lithium manganese oxide phase, best represented by the formula LiMn2O3.88. Diffraction methods show that the grain size decreases and multiple phases form after 850 electrochemical cycles at 0.2 C current. The microfractures are directly observed through microscopy studies as particle cracks propagate along the (1 1 1) planes, with clear lattice twisting observed along this direction. Long-term galvanostatic cycling results in increased charge-transfer resistance and capacity loss. Upon preparing samples with controlled oxygen contents, LiMn2O4.03 and LiMn2O3.87, the mechanical failure of the lithium manganese oxide can be correlated to the oxygen vacancies in the materials, providing guidance for better synthesis methods.
Species Variation in the Mechanisms of Mesenchymal Stem Cell-Mediated Immunosuppression (2009)

Bone marrow-derived mesenchymal stem cells (MSCs) hold great promise for treating immune disorders because of their immunoregulatory capacity, but the mechanism remains controversial. As we show here, the mechanism of MSC-mediated immunosuppression varies among different species. Immunosuppression by human- or monkey-derived MSCs is mediated by indoleamine 2,3-dioxygenase (IDO), whereas mouse MSCs utilize nitric oxide, under the same culture conditions. When the expression of IDO and inducible nitric oxide synthase (iNOS) were examined in human and mouse MSCs after stimulation with their respective inflammatory cytokines, we found that human MSCs expressed extremely high levels of IDO, and very low levels of iNOS, whereas mouse MSCs expressed abundant iNOS and very little IDO. Immunosuppression by human MSCs was not intrinsic, but was induced by inflammatory cytokines and was chemokine-dependent, as it is in mouse. These findings provide critical information about the immunosuppression of MSCs and for better application of MSCs in treating immune disorders.
Semantic role labeling using complete syntactic analysis (2005)

In this paper we introduce a semantic role labeling system constructed on top of the full syntactic analysis of text. The labeling problem is modeled using a rich set of lexical, syntactic, and semantic attributes and learned using one-versus-all AdaBoost classifiers.
Co-expression of S100A14 and S100A16 correlates with a poor prognosis in human breast cancer and promotes cancer cell invasion (2015)

To our knowledge, this is the first report showing the correlation of expression of S100A14, S100A16, and co-expression of these proteins with poor prognosis of breast cancer patients. In addition, our findings indicate that S100A14 and S100A16 can promote invasive activity of breast cancer cells via an interaction with cytoskeletal dynamics. S100A14 and S100A16 might be prognostic biomarkers and potential therapeutic targets for breast cancer.
Foetal and neonatal alloimmune thrombocytopaenia (2006)

Foetal/neonatal alloimmune thrombocytopaenia (NAIT) results from maternal alloimmunisation against foetal platelet antigens inherited from the father and different from those present in the mother, and usually presents as a severe isolated thrombocytopaenia in otherwise healthy newborns. The incidence has been estimated at 1/800 to 1/1000 live births. NAIT has been considered to be the platelet counterpart of Rh Haemolytic Disease of the Newborn (RHD). Unlike RHD, NAIT can occur during a first pregnancy. The spectrum of the disease may range from sub-clinical moderate thrombocytopaenia to life-threatening bleeding in the neonatal period. Mildly affected infants may be asymptomatic. In those with severe thrombocytopaenia, the most common presentations are petechiae, purpura or cephalohaematoma at birth, associated with major risk of intracranial haemorrhage (up to 20% of reported cases), which leads to death or neurological sequelae. Alloimmune thrombocytopaenia is more often unexpected and is usually diagnosed after birth. Once suspected, the diagnosis is confirmed by demonstration of maternal antiplatelet alloantibodies directed against a paternal antigen inherited by the foetus/neonate. Post-natal management involves transfusion of platelets devoid of this antigen, and should not be delayed by biological confirmation of the diagnosis (once the diagnosis is suspected), especially in case of severe thrombocytopaenia. Prompt diagnosis and treatment are essential to reduce the chances of death and disability due to haemorrhage. Due to the high rate of recurrence and increased severity of the foetal thrombocytopaenia in successive pregnancies, antenatal therapy should be offered. However, management of high-risk pregnancies is still a matter of discussion.
Ensemble of Trusted Firmware Services based on TPM (2011)

Firmware directly drives hardware, and vulnerable firmware might lead to the operating system crash. As firmware faces the increasingly attack, how to build the trusted firmware platform has become the research hot in computer security community. In this paper, ensemble of trusted firmware services under UEFI are discussed, which combines TPM and firmware for checking code modification, user authentication, hard disk attestation and real time security alert. The proposed approach exploits the root of trust, compares the hash value with platform registers for determining the code modification, authenticates user identity by matching fingerprint, identifies the hard disk through encrypted disk ID, and reports the real-time intrusion via security monitoring firmware. Experiments show that the proposed methods are feasible, and capable of constructing the trusted firmware platform.
El impacto de las migraciones contemporáneas en la radio española. La representación de la interculturalidad (2005)

O presente trabalho analisa como as radios convencionais espanholas, tambem conhecidas como radios para todos os publicos, refletem as migracoes contemporâneas e as relacoes interculturais. Para tanto, combinamos os dados do observatorio Migracom sobre a presenca e tratamento informativo e os informes sobre a oferta radiofonica, elaborados pelo Consell de l'Audiovisual de Catalunya. A partir da perspectiva da informacao radiofonica, constatou-se que o fator de proximidade influi na selecao tematica e enfoque (politico,social, economico). Ao contrario, no âmbito das programacoes evidenciou-se a falta de politicas e estrategias consolidadas que permitam abordar os perfis da nova sociedade espanhola. A situacao exige propostas de atuacao, mesmo que seja em forma de reflexao.
Stereoselective β-Hydrogen Elimination from Nickel(II)−<i>N</i>-Glycoside Complexes (1997)

Octahedral nickel(II)-N-glycoside complexes of glucose, galactose, mannose, and talose were synthesized and analyzed by electrospray ionization (ESI). A resulting tricoordinate species generated from the octahedral complex was subjected to collision-induced dissociation. A highly stereoselective dissociation pathway involving beta-hydrogen elimination and cross-ring cleavages was observed in complexes possessing equatorial C-2 substituents. (2)H- and (13)C-labeling experiments indicate that the hydrogen on C-2 and a labile proton are involved in the beta-hydrogen elimination. Additionally, C-4, C-5, and C-6 are shown to be lost from the monosaccharide as a result of the cross-ring cleavages. A mechanism is postulated to explain the stereoselectivity of this dissociation.
The Adenovirus L4-22K Protein Is Multifunctional and Is an Integral Component of Crucial Aspects of Infection (2012)

A variety of cellular and viral processes are coordinately regulated during adenovirus (Ad) infection to achieve optimal virus production. The Ad late gene product L4-22K has been associated with disparate activities during infection, including the regulation of late gene expression, viral DNA packaging, and infectious virus production. We generated and characterized two L4-22K mutant viruses to further explore L4-22K functions during viral infection. Our results show that L4-22K is indeed important for temporal control of viral gene expression not only because it activates late gene expression but also because it suppresses early gene expression. We also show that the L4-22K protein binds to viral packaging sequences in vivo and is essential to recruit two other packaging proteins, IVa2 and L1-52/55K, to this region. The elimination of L4-22K gave rise to the production of only empty virus capsids and not mature virions, which confirms that the L4-22K protein is required for Ad genome packaging. Finally, L4-22K contributes to adenovirus-induced cell death by regulating the expression of the adenovirus death protein. Thus, the adenovirus L4-22K protein is multifunctional and an integral component of crucial aspects of infection.