gebhart/patrepeval huggingface dataset:
Cancer Specific Proliferating Cell Nuclear Antigen as a Novel Diagnostic Marker for the Detection of Breast Cancer (2003)

Our laboratory has demonstrated the presence of different isoforms of proliferating cell nuclear antigen (PCNA) that display both acidic and basic isoelectric points (pI) . Analysis of PCNA by two- dimensional polyacrlamide gel electrophoresis (2D PAGE) from both malignant and non-malignant breast cells and tissues established the exclusive presence of the acidic form of PCNA in malignant cells (which is now referred to as the cancer-specific form of PCNA or csPCNA). Additionally, a basic form of PCNA was also observed in the malignant cells, but this isoform was the only isoform found in non-malignant cells and tissues. Testing of numerous other malignant and non-malignant breast cells suggested that the csPCNA would be an excellent prognostic indictor of breast cancer. Further investigation confirmed that a 29 amino acid fragment derived from the PCNA binding domain of the XPG protein interacted with csPCNA and not the basic PCNA isoform. This led us to believe that the xPG peptide would be a specific and sensitive probe that would enable us to identify csPCNA in different tissue and serum samples. It is therefore our goal in this research to develop an enzyme linked immunosorbent assay (ELISA) that utilizes the 29 amino acid fragment of XPG to detect the presence of csPCNA in cells, tissues, and sera. Additionally, we plan on using the XPG peptide for immunocytochemical (IHC) staining allowing us to look for csPCNA in tissues of patients with breast cancer and uncertain malignant diagnosis. Following development of these assays, we will then screen our breast tissue and serum repositories, which we have been generating, and correlate our finding with other prognostic factors such as BECAl and 2, p53, p2(exp kip), and estrogen receptor status and thus validate the usefulness of csPCNA as an earl diagnostic marker in breast cancer. 14 SUBJECT TERMS 15. NUMBER OF PAGES
Micronized Sorghum Grain. I. Influence on Feedlot Performance of Cattle (1975)

Three feedlot trails involving 64 steers and 30 heifers were conducted to study micronized sorghum grain for finishing cattle. Trials 1 and 2 compared micronized (MS) to dry rolled sorghum (DRS), and trial 3 compared three densities of MS (232, 322 and 412 g/l) to DRS. Evaluation was based on feedlot performance, carcass merit and volatile fatty acid analysis in trials 1 and 2, and included net energy (NEm and NEg) in trial 3. In trial I, MS produced an improved feed efficiency (feed/kg gain) (P< .10) with no significant differences in gain or feed intake. In trial 2, intake was lower (P< .10) on MS with no significant difference in gain or efficiency. In trial 3, there were no significant differences for intake, gain or feed conversion. In all three trials, the cattle fed MS showed a consistent trend to consume less feed, but gain at the same rate, showing an improved feed efficiency. Net energy values in trial 3 favored the MS treatments.
Alteration of the protein synthesis pattern in <i>Neurospora crassa</i> cells by hyperthermal and oxidative stress (1987)

Neurospora crassa cells, grown at 28 °C for 14 h and heat shocked at 48 °C for 45 min, showed the synthesis of 11 heat-shock proteins (nHSPs) in one-dimensional electrophoretic profiles. Treatment with sodium arsenite induced the synthesis of two heat-shock proteins, nHSP70 and nHSP80, and a third, arsenite-specific protein, not induced by hyperthermia. Exposure to 0.5 or 1.0 mM H 2 O 2 led to the induction of two of the heat-inducible nHSP70 family polypeptides. Sodium selenite, used in concert with H 2 O 2 , and arsenite were observed to modulate that heat-shock response. In addition, H 2 O 2 , menadione, and the glutathione depleters diamide and diethyl maleate promoted the synthesis of another protein, designated oxidative stress-responsive protein (OSP). A DNA-binding protein, specific for Neurospora DNA, was also demonstrated in extracts of heat-shocked cells.
Pathogen-Derived Extracellular Vesicle-Associated Molecules That Affect the Host Immune System: An Overview (2018)

Recently, the interest in extracellular vesicles (EVs) released by pathogens like bacteria, fungi, and parasites has rapidly increased. Many of these pathogens actively modulate the immune responses of their host and there is accumulating evidence that pathogen-derived EV contribute to this process. The effects of pathogen-derived EV on the host immune system have been attributed to proteins, lipids, nucleic acids, and glycans contained in, or present on these EV. For example, toxins in bacterial EV can modulate pathogen clearance and antigen presentation, while EV-associated polysaccharides are potential vaccine targets because they induce protective immune responses. Furthermore, parasite EV-associated microRNA may increase parasite survival via host gene repression, and the lipid A moiety of LPS in bacteria-derived EV induces strong pro-inflammatory responses. Research on pathogen EV-associated molecules may pave new avenues to combat infectious diseases by immune intervention. This review provides an overview of the current knowledge of EV-associated molecules released by extracellular pathogens and their effects on the host immune system. The current focus and future hotspots of this rapidly expanding field will be highlighted and discussed.
Specific inhibition of eubacterial DNA ligase (1998)

(1998). Specific inhibition of eubacterial DNA ligase. Emerging Therapeutic Targets: Vol. 2, No. 1, pp. 39-41.
Graphene-based one-dimensional photonic crystal (2011)

A novel type of one-dimensional (1D) photonic crystal formed by an array of periodically located stacks of alternating graphene and dielectric stripes embedded into a background dielectric medium is proposed. The wave equation for the electromagnetic wave propagating in such a structure is solved in the framework of the Kronig-Penney model. The frequency band structure of the 1D graphene-based photonic crystal is obtained analytically as a function of the filling factor and the thickness of the dielectric between the graphene stripes. The photonic frequency corresponding to the electromagnetic wave localized by a defect of the photonic crystal formed by an extra dielectric placed in the position of one stack of alternating graphene and dielectric stripes is obtained.
Pharmacology and neurochemistry of buspirone. (1982)

Biochemically, buspirone is unlike the benzodiazepines in that it neither stimulates nor inhibits 3H-benzodiazepine binding, does not affect the influence of GABA or halide anion on 3H-benzodiazepine binding, and does not interfere with GABA binding or uptake. Buspirone lacks anticonvulsant activity, interacts minimally with CNS depressants, and does not cause muscle relaxation. Its tranquilizing activity is characterized by the ability to (1) tame aggressive rhesus monkeys, (2) block conditioned avoidance responding in the rat, (3) inhibit shock-induced fighting in the mouse, and (4) attenuate shock-induced suppression of drinking in the rat. In vitro binding experiments indicate that buspirone lacks significant activity at several binding sites. It appears to interact only with the dopaminergic system and possesses properties that are similar to both dopamine agonists and dopamine antagonists.
Development of a non-invasive blood glucose monitor: application of artificial neural networks for signal processing (2002)

We have developed a noninvasive blood glucose measuring instrument, based on application of the Optical Bridge/sup TM/ in the near-infrared region. This exploratory research is an endeavor to evaluate the possibility of increasing the performance of the noninvasive glucose monitor by employing Artificial Neural Networks (ANN). The objective of this research is to design an ANN to interpret the instrument's outputs as well as the system parameters, and correlate them with blood glucose levels. The main hypothesis of this project is that such an ANN can be designed to improve the performance of this instrument.
The purification, characterization, and activation of phosphoenolpyruvate carboxykinase from chicken liver mitochondria. (1982)

The enzyme phosphoenolpyruvate carboxykinase has been purified from chicken liver mitochondria. This purification includes a pseudo-affinity column step utilizing Sepharose 4B-blue dextran which binds the enzyme. The enzyme elutes with ITP to yield protein which is greater than 98% pure. The enzyme has Mr = 75,400 +/- 200 estimated by high speed sedimentation equilibrium and 70,500 +/- 500 estimated by reduced sodium dodecyl sulfate-polyacrylamide gels. The enzyme is abnormally retarded on molecular exclusion resins yielding low apparent molecular weight values. The amino acid analysis indicates that the enzyme has a high proline content and a high tryptophan content and contains 9 mol of cysteine/mol of enzyme. No disulfide bonds were detected. The extinction coefficient (epsilon 1% 280 = 16.5 +/- 0.1) reflects the high tryptophan content. The Svedberg coefficient (s20,w = 4.63 +/- 0.03 S) is consistent with a globular protein of Mr = 70,500-75,400. The activation of the enzyme was investigated by steady state kinetics. The carboxylation reaction has an activation energy of 17.6 kcal/mol. There is no requirement of a monovalent cation for activity. A thiol is necessary for maximal activity, although apparently not to reduce disulfide bonds within the enzyme. Incubation with dithiothreitol stabilizes enzymatic activity but beta-mercaptoethanol facilitates loss of activity. The kinetics of activation by Mn2+ was performed. The Ks value for phosphoenolpyruvate (300 microM) decreases to an apparent Km of 67 microM with increasing concentrations of Mn2+. The concentration of Mn2+ does not affect the interaction of HCO-3 with the enzyme, however. Analysis of data in terms of free IDP indicates that increasing Mn2+ decreases the Km of IDP but analysis as MnIDP indicates the Km,app of MnIDP is independent of the Mn2+ concentration. The enzyme interacts with Mn2+ with a KA = 67 microM and the Km,app decreases to a value of 8 microM with saturating substrates. The substrate analogue (Z)-3-fluorophosphoenolpyruvate is a good substrate for the reaction (Km = 30 microM) with 27% Vmax compared to P-enolpyruvate (Km = 180 microM). Except for 3-bromophosphoenolpyruvate, other analogues have shown weak competitive or noncompetitive inhibition. Potential analogues of oxalacetate (succinate, citrate, isocitrate, malate, and alpha-ketoglutarate) all elicit weak (greater than 15 mM) inhibition.
Effect of oral Bacillus coagulans administration on the density of vancomycin-resistant enterococci in the stool of colonized mice (2001)

This study suggests a potential role for biotherapeutic agents as a means to reduce the density of VRE intestinal colonization.
Multi-Level Cartooning for Context-Aware Privacy Protection in Visual Sensor Networks (2014)

Our solution to the MediaEval 2014 Visual Privacy Task [4] is a privacy-preserving video filter that is able to maintain a high intelligibility level in surveillance systems while provid-ing a reasonable privacy protection level to monitored peo-ple and a pleasant view to observers. This paper describes our context-aware method that is based on cartooning and pixelation effects. Subjective evaluation results are also pre-sented to demonstrate the performance of our algorithm. 1.
Novel Method to Label Solid Lipid Nanoparticles with <sup>64</sup>Cu for Positron Emission Tomography Imaging (2011)

Solid lipid nanoparticles (SLNs) are submicrometer (1-1000 nm) colloidal carriers developed in the past decade as an alternative system to traditional carriers (emulsions, liposomes, and polymeric nanoparticles) for intravenous applications. Because of their potential as drug carriers, there is much interest in understanding the in vivo biodistribution of SLNs following intravenous (i.v.) injection. Positron emission tomography (PET) is an attractive method for investigating biodistribution but requires a radiolabeled compound. In this work, we describe a method to radiolabel SLN for in vivo PET studies. A copper specific chelator, 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N',N'',N'''-tetraacetic acid (BAT), conjugated with a synthetic lipid, was incorporated into the SLN. Following incubation with (64)CuCl(2) for 1 h at 25 °C in 0.1 M NH(4)OAc buffer (pH 5.5), the SLNs (∼150 nm) were successfully radiolabeled with (64)Cu (66.5% radiolabeling yield), exhibiting >95% radiolabeled particles following purification. The (64)Cu-SLNs were delivered intravenously to mice and imaged with PET at 0.5, 3, 20, and 48 h post injection. Gamma counting was utilized post imaging to confirm organ distributions. Tissue radioactivity (% injected dose/gram, %ID/g), obtained by quantitative analysis of the images, suggests that the (64)Cu-SLNs are circulating in the bloodstream after 3 h (blood half-life ∼1.4 h), but are almost entirely cleared by 48 h. PET and gamma counting demonstrate that approximately 5-7%ID/g (64)Cu-SLNs remain in the liver at 48 h post injection. Stability assays confirm that copper remains associated with the SLN over the 48 h time period and that the biodistribution patterns observed are not from free, dissociated copper. Our results indicate that SLNs can be radiolabeled with (64)Cu, and their biodistribution can be quantitatively evaluated by in vivo PET imaging and ex vivo gamma counting.
Particle Adhesion (2002)

Whenever a curved surface interacts with another surface, the principles of adhesion are at work. From the cells in your body to the dust on your glasses, intermolecular forces cause materials to attract one another. Elastic deformations resulting from these adhesive interactions store strain that can be liberated during particle detachment. Time d
Genocop III: a co-evolutionary algorithm for numerical optimization problems with nonlinear constraints (2002)

During the last two years several methods have been proposed for handling nonlinear constraints by genetic algorithms for numerical optimization problems; most of them were based on penalty functions. However, the performance of these methods is highly problem-dependent; moreover, many methods require additional tuning of several parameters. We present a new optimization system (Genocop III), which is based on concepts of co-evolution and repair algorithms. We present the results of the system on a few selected test problems and discuss some directions for further research.
Japanese encephalitis virus (JEV): Potentiation of lethality in mice by microwave radiation (1991)

The expression of Japanese Encephalitis Virus (JEV) lethality in mice requires entry of the virus into the central nervous system. This entry is presumably through the capillary endothelial cells (CEC), because entry between CECs is inhibited by bands of circumferential tight-junctions. A viremic stage occurs during the first 4 to 5 days after JEV administration in mice, and both microwave radiation (2.45-GHz, continuous wave, 10-min exposure) and hypercarbia were employed to increase CEC permeability to JEV. Exposure to microwaves at power densities of 10-50 mW/cm2 resulted in a dose-dependent increase in JEV-induced lethality. Mice did not become tolerant or sensitized to microwave potentiation of JEV-induced mortality because 4 daily exposures at 10 or 50 mW/cm2 (SARS, approximately 24-98 W/kg) did not alter the lethality pattern to subsequent microwave radiation of JEV-exposed animals. Similarly, hypercarbia (5, 10, and 20% CO2) was observed to produce a dose-dependent increase in JEV-induced lethality. Both microwave radiation and hypercarbia are thought to promote pinocytosis in CNS capillary endothelial cells. This may be one mechanism by which they enhance JEV-induced lethality in adult Swiss-Cox mice.
Nanosize fabrication using etching of phase-change recording films (2004)

An etching technique called phase-change etching was developed. In this technique, only crystalline regions in a phase-change recording film are selectively etched by an alkaline solution, and amorphous regions remain on the sample surface, which means that a phase-change recording film can be used as a resist for pattern formation. By combination of this technique and phase-change recording, fabrication of the dot pattern with a size of about 1∕10 of the fabricating spot was demonstrated. This result indicates the possibility of nanosize fabrication using the phase-change etching technique.
Alketas (2017)

Abstract Alketas was the author of On the monuments of Delphi in at least two books.
Principal Component Analysis with Contaminated Data: The High Dimensional Case (2010)

We consider the dimensionality-reduction problem (finding a subspace approximation of observed data) for contaminated data in the high dimensional regime, where the number of observations is of the same magnitude as the number of variables of each observation, and the data set contains some (arbitrarily) corrupted observations. We propose a High-dimensional Robust Principal Component Analysis (HR-PCA) algorithm that is tractable, robust to contaminated points, and easily kernelizable. The resulting subspace has a bounded deviation from the desired one, achieves maximal robustness -- a breakdown point of 50% while all existing algorithms have a breakdown point of zero, and unlike ordinary PCA algorithms, achieves optimality in the limit case where the proportion of corrupted points goes to zero.
MAJOR: Flexible tool development with aspect-oriented programming (2009)

Developing and maintaining tools for profiling, debugging, testing, and reverse engineering can be difficult when using low-level techniques, such as bytecode instrumentation. We promote tool development in Java using high-level aspect-oriented programming. We demonstrate that the use of aspects yields concise tools that are easy to develop, extend, and maintain, because low-level instrumentation details are hidden from the developer. We introduce MAJOR, a new tool for comprehensive aspect weaving, which ensures that aspects are woven into all classes executing in a Java Virtual Machine, including those in the standard Java class library. MAJOR includes the pluggable module CARAJillo, which supports efficient access to a complete and customizable calling context representation. Both distinguishing features of MAJOR - comprehensive aspect weaving and efficient access to complete calling information - are essential in the aforementioned domains.
Structure and the properties of 18α,19βH-ursane derivatives with a lactone bridge to ring E (1980)

Oxidation of ketolactone II in alkaline medium led predominantly to hydroxy acid VI with an oxabicycloheptane arrangement of the E ring. Further oxidation of hydroxy acid VI with lead tetraacetate or the pyrolysis of its diacetate, VII , gave ketone XV which on further oxidation gave either lactone XVIII or XXIV , depending on conditions. The structure of both lactones was confirmed by reduction to tetrol XIX or pentol XXV , from lactone XXIV diketone XXXI was also obtained.
Convenient preparation of N,N-dimethylacetamide dimethyl acetal (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConvenient preparation of N,N-dimethylacetamide dimethyl acetalRobert G. Salomon and Swadesh R. RaychaudhuriCite this: J. Org. Chem. 1984, 49, 19, 3659–3660Publication Date (Print):September 1, 1984Publication History Published online1 May 2002Published inissue 1 September 1984https://pubs.acs.org/doi/10.1021/jo00193a045https://doi.org/10.1021/jo00193a045research-articleACS PublicationsRequest reuse permissionsArticle Views1831Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Inter-Personal Relations in the Building Industry (1946)

Although building is a vast industry, employing thousands of men and involving the fabrication and assembling of great quantities of raw materials, the basic human relationships in the industry are for the most part informal and unstandardized. Inter-personal relations in building achieve their essential character not only from the technological organization of the industry with its itinerant patterns of manufacture and handicraft techniques, but also from an accumulation of attitudes, sentiments and traditions which form the culture of the trades. Perhaps more than workers in any other industry, the building workers function as independent units, each worker pursuing employment and making arrangements to apply his skills according to personal contacts, personal preference, and a personal schedule. Due to the fact that building projects are of relatively brief duration1 and that a new labor force is assembled for each job, there is an almost continuous turnover, producing separate employment patterns for ...
Photocatalysis by Nanoparticles of Titanium Dioxide for Drinking Water Purification: A Conceptual and State-of-Art Review (2013)

To overcome the water pollution problems, and to meet stringent environmental regulations, scientist and researchers have been focusing on the development of new water purification processes. One such group of new technologies is advanced oxidation processes (AOPs). Among the AOPs, titanium dioxide photocatalysis has been widely studied on lab scale by the researchers for decontamination of drinking water. In the present chapter, a conceptual as well as state-of-art review of titanium dioxide photocatalysis for water purification has been discussed.
The therapeutic potential of cannabis in multiple sclerosis (2003)

There has been renewed interest in the therapeutic applications of cannabis, and people, particularly those with multiple sclerosis, claim that it may offer benefit in symptom control. Cannabis exerts many of its effects because it taps into an endogenous cannabinoid system. Recent advances have begun to shine light on the biology of this system and may support some of the anecdotal medical claims. The problem with cannabis as a drug is that both the positive and negative aspects are largely the work of the same receptor. However, it may be possible to avoid these through modulation of the endogenous system. Cannabinoids provide a novel therapeutic target, not only for controlling symptoms, but also slowing disease progression through inhibition of neurodegeneration, which is the cause of accumulating irreversible disability.
Lifestyle Intervention in Overweight Individuals With a Family History of Diabetes (1998)

Although initially successful, the interventions studied here were not effective in producing long-term changes in behavior, weight, or physiological parameters. However, weight loss from 0 to 2 years reduced the risk of developing type 2 diabetes. Since modest weight loss significantly reduced risk of type 2 diabetes, further research is needed to determine how best to increase the percentage of subjects achieving at least a modest weight loss.