gebhart/patrepeval huggingface dataset:
Residues flanking the COOH-terminal C-region of a model eukaryotic signal peptide influence the site of its cleavage by signal peptidase and the extent of coupling of its co-translational translocation and proteolytic processing in vitro. (1990)

The polar, COOH-terminal c-region of signal peptides has been considered to be most important for influencing the efficiency and fidelity of signal peptidase cleavage while the hydrophobic core or h-region appears indispensable for initiating translocation. To identify structural features of residues flanking the c-region that influence the fidelity and efficiency of signal peptidase cleavage as well as co-translational translocation, we introduced six amino acid substitutions into the COOH terminus of the hydrophobic core and seven substitutions at the NH2 terminus of the mature region (the +1 position) of a model eukaryotic preprotein-human pre(delta pro)apoA-II. This preprotein contains several potential sites for signal peptidase cleavage. The functional consequences of these mutations were assayed using an in vitro co-translational translocation/processing system and by post-translational cleavage with purified, detergent-solubilized, hen oviduct signal peptidase. The efficiency of translocation could be correlated with the hydrophobic character of the residue introduced at the COOH terminus of the h-region. Some h/c boundary mutants underwent co-translational translocation across the microsomal membrane with only minimal cleavage yet they were cleaved post-translationally by hen oviduct signal peptidase more efficiently than other mutants which exhibited a high degree of coupling of co-translational translocation and cleavage. These data suggest that features at the COOH terminus of the h-domain can influence "presentation" of the cleavage site to signal peptidase. The +1 residue substitutions had minor effects on the extent of co-translational translocation and processing. However, these +1, as well as h/c boundary mutations, had dramatic effects on the site of cleavage chosen by signal peptidase, indicating that residues flanking the c-region of this prototypic eukaryotic signal peptide can affect the fidelity of its proteolytic processing. The site(s) selected by canine microsomal and purified hen oviduct signal peptidase were very similar, suggesting that "intrinsic" structural features of this prepeptide can influence the selectivity of eukaryotic signal peptidase cleavage, independent of the microsomal membrane and associated translocation apparatus.
Propagation of interface waves in a clay soil (2004)

Measurements and analyses on the propagation of interface waves, previously conducted on a sandy beach in Monterey, CA, are extended to a clay soil in Oxford, MS, in support of research on seismic sonar. Data are presented on attenuation versus lateral range and frequency, which may result from attenuation and scattering, as well as refraction and modal effects. Data are also presented on spatial coherence and group velocity, as a function of angle, along semi-circular arcs, also as a function of lateral range. The interface waves were generated in pulses, with both moving coil and moving mass sources (shakers), and were measured with three axis seismometers, at frequencies ranging from a few tens of Hz to several hundred Hz, at lateral ranges from a few meters to a few tens of meters. The sources excited numerous seismo-acoustic wave types; but the analysis focused on (1) Rayleigh waves having radial and vertical components in the vertical plane, and (2) transverse waves in the horizontal plane, thought to be Love waves. The data are interpreted with respect to environmental ‘‘ground truth’’ measurements. [Work supported by the U.S. Marine Corps Systems Command.]
An actin barrier to resealing (2001)

Plasma membrane disruption is a common form of cell injury in many normal biological environments, including many mammalian tissues. Survival depends on the initiation of a rapid resealing response that is mounted only in the presence of physiological levels of extracellular Ca(2+). Vesicle-vesicle and vesicle-plasma membrane fusion events occurring in cortical cytoplasm surrounding the defect are thought to be a crucial element of the resealing mechanism. However, in mammalian cells, the vesicles used in this fusion reaction (endosomes/lysosomes) are not present in a 'pre-docked' configuration and so must be brought into physical contact with one another and with the plasma membrane. We propose that a requisite prelude to fusion is the disassembly in local cell cortex of the physical barrier constituted by filamentous actin. Consistent with this hypothesis, we found that rat gastric epithelial (RGM1) cell cortical staining with phalloidin was apparently reduced at presumptive disruption sites. Moreover, flow cytofluorometric analysis of wounded RGM1 populations revealed a small, but significant, Ca(2+)-dependent reduction in whole cell phalloidin staining. The functional significance of this disruption-induced depolymerization response was confirmed in several independent tests. Introduction into RGM1 cells of the filamentous actin-depolymerizing agent, DNase1, enhanced resealing, although cytochalasin treatment, by itself, had no effect. By contrast, when the filamentous actin cytoskeleton was stabilized experimentally, using phalloidin or jasplakinolide, resealing was strongly inhibited. Cells in wounded cultures displayed an enhanced cortical array of filamentous actin, and resealing by such cells was enhanced strongly by both cytochalasin and DNase 1, demonstrating the specific reversibility of a biologically mediated, polymerization-induced inhibition of resealing. We conclude that localized filamentous actin disassembly removes a cortical barrier standing in the way of membrane-membrane contacts leading to resealing-requisite homotypic and exocytotic fusion events.
Four-colour photometry of eclipsing binaries. XXXI: Light curves of EM carinae (1989)

Les courbes de lumiere uvby de la binaire detachee, a eclipse EM Car sont analysees pour determiner les parametres physiques et les elements orbitaux du systeme. Les donnees photometriques collectees entre 1981 et 1988 sont presentees
Mass spectrometric studies of cisplatin‐induced changes of hemoglobin (2003)

This study reports on structural changes of hemoglobin (Hb) that were induced by cisplatin binding. Two techniques, nanoelectrospray quadrupole time-of-flight mass spectrometry (nanoES-MS) and high-performance liquid chromatography with inductively coupled plasma mass spectrometry (HPLC/ICPMS), were developed to facilitate this study. Nanospray MS analyses of cisplatin and Hb reaction mixtures demonstrated that the ion at m/z 616.5, the heme group, increased with an increase of cisplatin concentration, indicating the loss of heme groups from the intact protein. This conclusion was also supported by the increase of cisplatin-alpha or -beta complex formation. The change of the Hb-bound Fe was further investigated by monitoring Fe signals using size-exclusion HPLC/ICPMS. After incubation with cisplatin at clinically relevant concentrations, under physiological conditions, the amount of Fe bound to Hb was reduced while formation of cisplatin-Hb complexes increased. Flow-injection ICPMS analysis of the Fe contents in the low molecular weight fraction (<3000 Da) of the reaction mixtures after size fractionation further demonstrated a corresponding increase of Fe with the increase of cisplatin concentrations. HPLC/ICPMS detected three Hb-cisplatin complexes, one of which eluted at the same retention time as Hb while the other two complexes eluted later than Hb. With clinically relevant concentrations of cisplatin (0.05-1.0 microM) and 10 microM of Hb, the concentrations of the Hb-cisplatin complexes were determined in the range 0.1-64 nM. These results, obtained from nanoES-MS, HPLC/ICPMS, and FIA-ICPMS, demonstrate that cisplatin binding to Hb resulted in the dissociation of the heme group from the intact protein.
Psychophysical evaluation of animated facial expressions (2005)

The human face is capable of producing an astonishing variety of expressions---expressions for which sometimes the smallest difference changes the perceived meaning noticably. Producing realistic-looking facial animations that are able to transport this degree of complexity continues to be a challenging research topic in computer graphics. One important question that remains to be answered is: When are facial animations good enough? Here we present an integrated framework in which psychophysical experiments are used in a first step to systematically evaluate the perceptual quality of computer-generated animations with respect to real-world video sequences. The result of the first experiment is an evaluation of several animation techniques in which we expose specific animation parameters that are important for perceptual fidelity. In a second experiment we then use these benchmarked animations in the context of perceptual research in order to systematically investigate the spatio-temporal characteristics of expressions. Using such an integrated approach, we are able to provide insights into facial expressions for both the perceptual and computer graphics community.
Volvulus of Caecum, associated with Putrid Puerperal Endometritis and Gangrenous Vulvitis (1941)

and the ulcer was healed within three weeks. He has been seen since on many occasions, and when last examined, seven years after the operation, he was in goQd general condition, able to do some work and to walk at least 300 yards without trouble.
An Intelligent System to Detect Drilling Problems Through Drilled Cuttings Return Analysis (2010)

Abstract Cuttings return analysis is an important tool to detect and prevent problems during the petroleum well drilling process. Several measurements and tools have been developed for drilling problems detection, including mud logging, PWD and downhole torque information. Cuttings flow meters were developed in the past to provide information regarding cuttings return at the shale shakers. Their use, however, significantly impact the operation including rig space issues, interferences in geological analysis besides, additional personel required. This article proposes a non intrusive system to analyze the cuttings concentration at the shale shakers, which can indicate problems during drilling process, such as landslide, the collapse of the well borehole walls. Cuttings images are acquired by a high definition camera installed above the shakers and sent to a computer coupled with a data analysis system which aims the quantification and closure of a cuttings material balance in the well surface system domain. No additional people at the rigsite are required to operate the system. Modern Artificial intelligence techniques are used for pattern recognition and data analysis. Techniques include the Optimum-Path Forest (OPF), Artificial Neural Network using Multilayer Perceptrons (ANN-MLP), Support Vector Machines (SVM) and a Bayesian Classifier (BC). Field test results conducted on offshore floating vessels are presented. Results show the robustness of the proposed system, which can be also integrated with other data to improve the efficiency of drilling problems detection.
Real Estate Management System based on Blockchain (2020)

Real Estate management in India as well as in many parts of the world is a very inefficient process. Developing a secure central system that not only accelerates the process of land registration but also makes it efficient will be effective. This paper presents a blockchain-powered real estate management system that will provide a transparent, secure, and efficient system for real estate management. This system will include all the departments related to real estate management. It will store all the transactions on a distributed permissioned blockchain which will be very secure and will not be prone to hacking and this can be automated to a great extent. The system will be centralized connecting all the departments and will be decentralized for data storage. It is a practical solution to the real estate management problem.
Influence of Valley Floor Landforms on Stream Ecosystems (1989)

A hierarchical perspective of relationships be- tween valley floor landforms, riparian plant communi- ties, and aquatic ecosystems has been developed, based on studies of two fifth-order basins in the Cascade Moun- tains of Oregon. Retention of dissolved nitrogen and leaves were approximately 2-3 times greater in uncon- strained reaches than in constrained reaches. Both val- ley floor landforms and riparian plant communities in- fluenced the abundance of primary producers. Abun- dances of cutthroat and rainbow trout in unconstrained reaches were approximately twice those observed in con- strained valley floors. Valley floors are one of the most physically dynamic components of the landscape, incor- porating major agents of terrestrial disturbance and flu- vial disturbance. These corridors are major routes for the flux of water, sediments, nutrients, and species. Be- cause of their unique properties, valley floors play an important role in landscape ecology. Most definitions of riparian zones for land manage- ment or ecological research are based on a few se- lected hydrologic, topographic, edaphic, or vegetative attributes of riparian areas. Riparian zones have been investigated from the perspectives of erosion control by riparian vegetation, phreatophyte ecology, uptake of nu- trients or contaminants from groundwater, chemistry of water entering lakes and rivers, shading of headwa- ter streams, effects on aquatic invertebrates, migration routes for wildlife, habitat for water fowl, and fish habi- tat. All of these subjects are critical aspects of riparian ecology, but it is important to recognize the constraints of concepts or definitions of riparian zones developed for specific sets of objectives. In recent decades, ecologists and land use managers have recognized the importance of the structure and functions of riparian zones for both aquatic and terres- trial ecosystems (Knight and Bottorf 1984, Meehan and others 1977, Swanson and others 1982). Meehan and others (1977) defined riparian vegetation as any extra- aquatic vegetation that directly influences the stream environment. From an aquatic perspective, riparian zones are defined functionally as three-dimensional zones of direct interaction with aquatic ecosystems, extending outward from the channel to the limits of flooding and upward into the canopy of streamside vegetation (fig. 1). Examples of critical functions of riparian vegetation for stream ecosystems include shading, bank stabilization, uptake of nutrients, input of leaves and needles, reten- tion of particulate organic matter during high flows, and contribution of large wood.
Granulocyte-macrophage colony-stimulating factor activates macrophages derived from bone marrow cultures to synthesis of MHC class II molecules and to augmented antigen presentation function. (1988)

The effects of granulocyte-macrophage (GM)-CSF on the synthesis of MHC class II molecules and on the Ag presentation capacity by bone marrow derived macrophages (BMM phi) was investigated. BMM phi obtained by in vitro culture in the presence of macrophage-CSF were negative for synthesis of I-A molecules and induced the Ag-mediated proliferation of insulin-specific T clone cells with lower efficiency than splenic accessory cells. After pulse treatment with GM-CSF for 24 to 48 h, day 12 BMM phi exhibited highly efficient Ag presentation function which was superior to that induced by IFN-gamma. Expression of membrane-bound IL-1 was augmented significantly by GM-CSF, but not by IFN-gamma. However, the T cell clone used to probe for accessory cell function of BMM phi was not dependent on IL-1 for optimal proliferation. Concomitantly, GM-CSF induced the de novo synthesis of I-A molecules, although to a lesser extent than optimal doses of IFN-gamma. Thus GM-CSF appears to elicit properties in addition to Ia molecule synthesis and membrane IL-1 expression in BMM phi being essential for efficient accessory cell function to the T clone cells. The activation of BMM phi by GM-CSF was reversible and could be repeated. These data show that GM-CSF exerts a modulatory influence on preformed BMM phi, reversibly activating cells to Ia biosynthetic potential and pronounced accessory cell capacity, thus rendering the explanation unlikely that differentiation of precursor cells into a constitutively functional state had occurred.
Test point insertion for compact test sets (2002)

Efficient production testing is frequently hampered because (cores in) current complex digital circuit designs require too large test sets, even with powerful ATPG tools that generate compact test sets. Built-in Self-Test approaches often suffer from fault coverage problems, due to random-resistant faults, which can be improved successfully by means of Test Point Insertion (TPI). In this paper, we evaluate the effect of TPI for BIST on the compactness of ATPG generated test sets and it turns out that most often a significant test set size reduction can be obtained. We also propose a novel TPI method, specifically aimed at facilitating compact test generation, based on the ''test counting' technique. Experimental results indicate that the proposed method results in even larger and moreover more consistent reduction of test set sizes.
Nutritional requirements of human malignant (leukemic) cell lines: implications for adjuvant therapy. (1987)

Metabolic requirements of malignant cell lines derived from patients with chronic myelogenous and acute lymphoblastic leukemias were compared to those of proliferating normal cells (mitogen-stimulated human lymphocytes) and circulating blasts from acute myeloblastic and acute lymphoblastic leukemias. Requirements were judged by degree of amino acid (AA) utilization in short-term cultures and assessed by the effect of selective AA deprivation on cell growth. Cell growth was measured by DNA synthesis and growth rate analysis. Six AAs (serine, threonine, methionine, valine, phenylalanine, and lysine) were appreciably utilized (52-87%) by IM-9, CEM, MOLT-4, and K-562 cells, but little or no utilization of these or any other AAs were noted in HSB cells, in leukemic blasts, or in mitogen-stimulated normal lymphocytes in short-term culture. Omission of lysine from culture media greatly inhibited cell growth (DNA synthesis by 91%), and cell density (by 83%) of IM-9 cells. However, omission of lysine, valine, serine, threonine, methionine, or phenylalanine had less of an effect on CEM and MOLT-4 cell lines. These observations demonstrate that under the conditions used the IM-9 cell line is uniquely dependent on extracellular lysine levels in contrast to the other cell lines studied. This suggests that human malignancies other than acute lymphoblastic leukemia which exhibits an obligate dependence on extracellular asparagine might be manageable by enzymatic degradation in vivo or by dietary restriction of indispensable AAs.
Development of Peptide Nucleic Acid Probes for Detection of the HER2 Oncogene (2013)

Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA. We have synthesized 15/16-mer PNA probes to detect the HER2 mRNA. The performance of these probes to detect the HER2 target was evaluated by fluorescence imaging and fluorescence bead assays. The PNA probes have sufficiently discriminated between the wild type HER2 target and the mutant target with single base mismatches. Furthermore, the probes exhibited excellent linear concentration dependence between 0.4 to 400 fmol for the target gene. The results demonstrate potential application of PNAs as diagnostic probes with high specificity for quantitative measurements of amplifications or over-expressions of oncogenes.
Bifunctional thermoelectric tube made of tilted multilayer material as an alternative to standard heat exchangers (2013)

Enormously large amount of heat produced by human activities is now mostly wasted into the environment without use. To realize a sustainable society, it is important to develop practical solutions for waste heat recovery. Here, we demonstrate that a tubular thermoelectric device made of tilted multilayer of Bi(0.5)Sb(1.5)Te3/Ni provides a promising solution. The Bi(0.5)Sb(1.5)Te3/Ni tube allows tightly sealed fluid flow inside itself, and operates in analogy with the standard shell and tube heat exchanger. We show that it achieves perfect balance between efficient heat exchange and high-power generation with a heat transfer coefficient of 4.0 kW/m(2)K and a volume power density of 10 kW/m(3) using low-grade heat sources below 100°C. The Bi(0.5)Sb(1.5)Te3/Ni tube thus serves as a power generator and a heat exchanger within a single unit, which is advantageous for developing new cogeneration systems in factories, vessels, and automobiles where cooling of excess heat is routinely carried out.
From Paper Drawings to Computer-Aided Design (1985)

A Canadian feasibility study shows that the key to reliable and cost-effective automatic reading of drawings is detailed planning of an operator-assisted system.
Consistent linear speedups to a first solution in parallel state-space search (1990)

Consider the problem of exploring a large state-space for a goal state. Although many such states may exist, finding anyone state satisfying the requirements is sufficient. All methods known until now for conducting such search in parallel fail to provide consistent linear speedups over sequential execution. The speedups vary between sublinear to superlinear and from run to run. Further, adding processors may sometimes lead to a slow-down rather than speedup, giving rise to speedup anomalies. We present prioritizing strategies which yield consistent linear speedups and requires substantially smaller memory over other methods. The performance of these strategies is demonstrated on a multiprocessor.
Synthesis and Activity of Largazole Analogues with Linker and Macrocycle Modification (2008)

To characterize largazole's structural requirements for histone deacetylase (HDAC) inhibitory and antiproliferative activities, a series of analogues with modifications to the side chain or 16-membered macrocycle were prepared and biologically evaluated. Structure-activity relationships suggested that the four-atom linker between the macrocycle and octanoyl group in the side chain and the (S)-configuration at the C17 position are critical to repression of HDAC activity. However, the valine residue in the macrocycle can be replaced with alanine without significant loss of activity.