gebhart/patrepeval huggingface dataset:
Initiation of plus-strand DNA synthesis during reverse transcription of an avian retrovirus genome (1984)

Two in vitro approaches were used to investigate the priming of strong-stop plus DNA by Rous sarcoma virus. This 340-base DNA species is the first major plus-strand DNA product seen in both infected cells and in endogenous reactions of disrupted virions. In the first approach, we set up a reconstructed system in which strong-stop plus DNA was synthesized by reverse transcriptase from a high-molecular-weight minus-strand viral DNA template. This synthesis was shown to be strictly dependent on the addition of primers to the reaction mixture. The addition of high-molecular-weight RNA from both viral and cellular sources, as well as oligodeoxyguanylate, gave specific synthesis of strong-stop plus DNA, whereas the addition of oligodeoxycytidylate-oligodeoxyadenylate and viral 4S RNA did not. In the second approach, strong-stop plus DNA synthesized in melittin-permeabilized virions was examined on a high-resolution polyacrylamide gel. This DNA was shown to have ca. 11 to 13 ribonucleotides at its 5' end. These results indicate that strong-stop plus DNA is initiated on a preformed RNA primer.
Can dietary assessment in general practice target patients with unhealthy diets? (2000)

Diet is important in the aetiology and management of many conditions in primary care. Although valid dietary assessment is required for both clinical work and research, no dietary assessment instruments have been validated among patients seen in primary care. A range of simple self-completion dietary assessment questionnaires and established research instruments were compared with an accepted reference standard, a seven-day weighed record, in 111 subjects assessed in a practice nurse-run treatment room. Simple self-completion tools based on food groups and portion sizes perform as well (likelihood ratios for a positive test = 2 to 3) as much more time-consuming instruments. The error in using such instruments is comparable with the error of the standard itself. There is little justification for using time-consuming dietary assessment questionnaires, since simple tools are accurate enough to be clinically useful--to allow practice nurses to target patients for counselling and waste less time on inappropriate counselling--and also useful for research.
Addendum (1948)

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Analysis of the Receptor Binding Domain of Gpa1p, the G <sub>α</sub> Subunit Involved in the Yeast Pheromone Response Pathway (1997)

The Saccharomyces cerevisiae G protein alpha subunit Gpa1p is involved in the response of both MATa and MAT alpha cells to pheromone. We mutagenized the GPA1 C terminus to characterize the receptor-interacting domain and to investigate the specificity of the interactions with the a- and alpha-factor receptors. The results are discussed with respect to a structural model of the Gpa1p C terminus that was based on the crystal structure of bovine transducin. Some mutants showed phenotypes different than the pheromone response and mating defects expected for mutations that affect receptor interactions, and therefore the mutations may affect other aspects of Gpa1p function. Most of the mutations that resulted in pheromone response and mating defects had similar effects in MATa and MAT alpha cells, suggesting that they affect the interactions with both receptors. Overexpression of the pheromone receptors increased the mating of some of the mutants tested but not the wild-type strain, consistent with defects in mutant Gpa1p-receptor interactions. The regions identified by the mating-defective mutants correlated well with the regions of mammalian G(alpha) subunits implicated in receptor interactions. The strongest mating type-specific effects were seen for mutations to proline and a mutation of a glycine residue predicted to form a C-terminal beta turn. The analogous beta turn in mammalian G(alpha) subunits undergoes a conformational change upon receptor interaction. We propose that the conformation of this region of Gpa1p differs during the interactions with the a- and alpha-factor receptors and that these mating type-specific mutations preclude the orientation necessary for interaction with one of the two receptors.
Kinetic Phases in the Electron Transfer from P<sup>+</sup>Q<sub>A</sub><sup>-</sup>Q<sub>B</sub> to P<sup>+</sup>Q<sub>A</sub>Q<sub>B</sub><sup>-</sup> and the Associated Processes in <i>Rhodobacter sphaeroides </i>R-26 Reaction Centers (1998)

Electron transfer from P+QA-QB to form P+QAQB- was measured in Rhodobacter sphaeroides R-26 reaction centers (RCs) where the native primary quinone, ubiquinone-10 (UQA), was replaced by 2-methyl-3-phytyl-1,4-naphthoquinone (MQA). The native secondary quinone, UQ-10, was retained as UQB. The difference spectrum of the semiquinone MQA- minus UQB- absorption is very similar to that of MQ- minus UQ- in solution (398-480 nm). Thus, the absorption change provides a direct monitor of the electron transfer from MQA- to UQB. In contrast, when both QA and QB are UQ-10 the spectral difference between UQA- and UQB- arises from electrochromic responses of RC chromophores. Three kinetic processes are seen in the near UV (390-480 nm) and near-IR (740-820 nm). Analysis of the time-correlated spectra support the conclusion that the changes at tau1 approximately 3 micros are mostly due to electron transfer, electron transfer and charge compensation are mixed in tau2 approximately 80 micros, while little or no electron transfer occurs at 200-600 micros (tau3) in MQAUQB RCs. The 80-micros rate has been previously observed, while the fast component has not. The fast phase represents 60% of the electron-transfer reaction (398 nm). The activation energy for electron transfer is DeltaG approximately 3.5 kcal/mol for both tau1 and tau2 between 0 and 30 degrees C. In isolated RCs with UQA, if there is any fast component, it appears to be faster and less important than in the MQA reconstituted RCs.
Gold Nanoparticles Deposited on SiO<sub>2</sub>/Si(100):  Correlation between Size, Electron Structure, and Activity in CO Oxidation (2003)

Nanosize gold particles were prepared by Ar(+) ion implantation of 10-nm thick gold film deposited onto a SiO(2)/Si(100) wafer possessing no catalytic activity in the CO oxidation. Along with size reduction the valence band of the gold particles and the actual size were determined by ultraviolet- and X-ray photoelectron spectroscopy (UPS, XPS) and by transmission electron microscopy (TEM) as well as atomic force microscopy (AFM), respectively. The catalytic activity was determined in the CO oxidation. Energy distribution of the photoelectrons excited from 5d valence band of gold was strongly affected by Ar(+) implantation. This variation was interpreted by the redistribution of the valence band density of states (DOS). The intrinsic catalytic activity of the gold particles increased with decreasing size. When an Au/FeO(x) interface was created by FeO(x) deposition on large gold nanoparticles, a significant increase in the rate of the CO oxidation was observed. These data can be regarded as an experimental verification of the correlation between the catalytic activity and valence band density of states of gold.
A Tandem Carbanion Addition/Carbon−Carbon Bond Cleavage Yields Alkynyl Ketones (2005)

Carbanion addition to vinylogous acid triflates triggers carbon-carbon bond cleavage to form alkynyl ketones under mild conditions. Mechanistic factors affecting the cleavage event and its relationship to complementary fragmentations are discussed. A range of tethered keto-alkynes are obtained by a unified approach.
Production of a dense metal-ion stream for ion coating by a pulsed vacuum arc in a strong magnetic field (1993)

A dense metal-ion stream (∼1×1018/shot) was produced by a high-current, long-pulse vacuum arc (∼2 kA, ∼25 ms) in a strong magnetic field. The ion stream was transported 40 cm by the magnetic field to a substrate and then deposited on it. Fast film formation (∼200 Å/shot), such as TiN and TiC films, was achieved by this device using a Ti electrode discharge in N2 or CH4 gas.
Implementation of High-k / Metal Gate in High-Volume Manufacturing (2008)

Significant breakthroughs emerged in the field of the IC technology in the past decade. The backend of the line saw a transition to copper and ultra low-k interlayer dielectric for RC delay improvement with unique integration approaches such as dual damascene. Advanced Patterning Films have enabled the introduction of Double Patterning and Dual Exposure Lithography allowing line printing at sub-45nm node. Introduction of uniaxial strain at 90nm logic node and a combination of mobility enhancing stressors boosted logic cell performance to meet 45nm node logic requirements. Reliable gate oxynitride of only a few atomic monolayers is now routinely used in high performance devices. Nonetheless, Intel co-founder Gordon Moore stated that introducing high-k and metal gates to transistors is the biggest change in transistor technology in nearly 40 years, firmly pushing Moore's Law scaling back on track. Indeed, high-k and metal gate implementation has been one of the industry's grand challenges. Today this process is in high-volume manufacturing and this paper discusses key challenges associated with transition from oxynitride to high-k and metal gates.
Type VII collagen gene expression in human umbilical tissue and cells. (1993)

The results show that umbilical tissue and cells, specifically those from the Wharton's jelly, are relatively enriched in type VII collagen. There is differential spatial localization of this collagen in the fetal tissue. The novel finding is that cells, other than epithelial cells such as keratinocytes, are able to express the type VII collagen gene.
Lipid composition and synthesis of HaCaT cells, an immortalized human keratinocyte line, in comparison with normal human adult keratinocytes (1993)

Cultured keratinocytes are frequently employed for studies of epidermal lipid metabolism. Interpretation of experimental data may be complicated by donor to donor variability, the relatively short culture lifetime and variations between passages, problems that are not encountered to the same extent with immortalized cell lines. The present study was undertaken to compare the lipid composition and synthesis of normal human adult keratinocytes (NHAK) with HaCaT cells, a long-lived, spontaneously immortalized human keratinocyte line, in relation to proliferation and differentiation. No differences between the two cell types were observed: a) in total lipid content; b) in the distribution of major lipid classes during growth at 50%, 75% and 100% confluence; c) in cultures grown at 0.6 mM calcium, at which differentiation is retarded, or at 1.6 mM calcium, at which some differentiation takes place; d) in the incorporation of [14C] acetate into cellular lipids at confluence, or e) in the fatty acid composition of major cellular lipid classes. At 100% confluence NHAK and HaCaT cells differ in their cholesterol metabolism. At all stages of growth, cholesterol synthesis in HaCaT cells is more LDL-dependent than in NHAK. Furthermore, NHAK become less LDL-dependent at confluence whereas HaCaT cells do not. HaCaT cells also revealed a significantly larger fraction of phosphatidyl-ethanolamine, -serine and -inositol at 0.6 mM calcium concentration than NHAK. These findings suggest that HaCaT cells do not differentiate as well as NHAK in vitro and may therefore serve as a model for the study of lipid metabolism in cells defective in terminal differentiation.
Filling Out the Gaps: A Padding Algorithm for Transforming Out Timing Leaks (2006)

It has been shown that secret information can be leaked to external observers through covert timing channels. In this paper we are concerned with a kind of timing attack that wants to differentiate two processes, presented as probabilistic transition systems, by observing their timing behaviour. Our goal is to make the processes indistinguishable i.e. bisimilar, by adding virtual (dummy) states and transitions to the original processes (padding). Instead of padding the processes with whole virtual copies of their counterparts - as done by some padding algorithms - we present an algorithm that uses the bisimulation equivalence relation - computed as a lumping partition - as the main criterion to optimise the padding procedure.
WATER TRANSFERS AND THEIR IMPACTS: LESSONS FROM THREE COLORADO WATER MARKETS<sup>1</sup> (2003)

ABSTRACT: This paper presents an analysis of the effects of different institutional arrangements and economic environments on water markets. Characteristics of water rights transfers in the South Platte Basin of Colorado and transfers of shares of the Northern Colorado Water Conservancy District (NCWCD) are compared to show how different institutional arrangements can affect the types and size distributions of transfers. The characteristics of water rights transfers in the prosperous South Platte are then compared with water rights transfer characteristics in the economically marginal Arkansas River basin of Colorado to identify the effects of different economic environments. Finally, the economic losses from reductions in irrigated acreage resulting from water transfers are estimated for the South Platte and Arkansas and compared with purchase prices by municipalities. Transfers in the South Platte were to new uses in the same basin, while more recent transfers in the Arkansas were to out of basin users. Transfers of South Platte rights and especially NCWCD shares were small and continuous over time, while transfers in the Arkansas were dominated by a few very large transfers. The negative impacts are judged to be more severe in the Arkansas basin than in the South Platte. Purchase prices paid by municipalities substantially exceeded capitalized transitional losses in the selling areas. In the South Platte, gains and losses were in the same basin, while the Arkansas absorbed the losses, with the benefits going to the purchasing basin.
A Statistical Approach for Analyzing the Inhibiting Effect of Different Types of Crude Oil in CO2 Corrosion of Carbon Steel (2002)

Abstract The inhibiting effect of several crude oils on CO2 corrosion was studied at different crude oil/water ratios. A statistical analysis showed an important difference between paraffinic and asphaltenic crude oils from the mechanistic point of view, with new findings on the variables that mostly influence the corrosion inhibiting mechanism.
A study of bridging defect probabilities on a Pentium (TM) 4 CPU (2002)

Presents an experimental study of bridging fault locations on the Intel Pentium (TM) 4 CPU as determined by an inductive fault analysis tool. The study focuses on the location and distribution of probable bridging defects and attempts to explain the findings in the context of the characteristics of the design and its implementation. The coverage obtained against these faults by manually generated functional patterns is compared against that achieved by ATPG vectors.
Micropatterns of Chemisorbed Cell Adhesion-Repellent Films Using Oxygen Plasma Etching and Elastomeric Masks (2003)

Cellular micropatterning has become an important tool to precisely design cell-to-substrate attachment for cell biology studies, tissue engineering, cell-based biosensors, biological assays, and drug screens. This paper describes a new technique for micropatterning of cells that is based on the use of oxygen plasma as a patterning tool. The technique consists of (1) homogeneously grafting a glass substrate with a protein-repellent interpenetrating polymeric network (IPN) of poly(acrylamide) and poly(ethyleneglycol) [P(AAm-co-EG)] prepared with commercially available reagents and (2) selectively removing this coating using oxygen plasma. We use elastomeric stencils (i.e. self-sealing membranes with through-holes) and microchannels as removable masks for the selective oxygen plasma etch of the IPN areas that are not protected by the mask. The stencil or microchannels are peeled off to reveal cell-adhesive regions separated by the nonadhesive coating. Our method offers a convenient way of patterning a robust protein-repelling material, allows for independently controlling the chemistry of the regions reserved for cell attachment, and can be used to create coculture systems.
Human acoustic fingerprints: A novel biometric modality for mobile security (2014)

Recently, the demand for more robust protection against unauthorized use of mobile devices has been rapidly growing. This paper presents a novel biometric modality Transient Evoked Otoacoustic Emission (TEOAE) for mobile security. Prior works have investigated TEOAE for biometrics in a setting where an individual is to be identified among a pre-enrolled identity gallery. However, this limits the applicability to mobile environment, where attacks in most cases are from imposters unknown to the system before. Therefore, we employ an unsupervised learning approach based on Autoencoder Neural Network to tackle such blind recognition problem. The learning model is trained upon a generic dataset and used to verify an individual in a random population. We also introduce the framework of mobile biometric system considering practical application. Experiments show the merits of the proposed method and system performance is further evaluated by cross-validation with an average EER 2.41% achieved.
[Therapeutic effect of kangke injection on viral myocarditis and its anticoxsackie virus mechanism]. (1997)

RSF was an effective substance for regressing the "Pathologic status" of viral myocarditis.
Regional and Systemic Haemodynamic Effects of Some Vasopressins: Structural Features of the Hormone which Prolong Activity (1975)

Cardiac output and regional blood flows to myocardium, gut, uterus and kidney were determined in anaesthetised female rats by a single injection of 86RbCl. The haemodynamic responses were measured at various time intervals up to 2 h after single I.V. injections of lysine-vasopressin and the following of its analogues: a) with extended peptide chains at the N-terminal (including "hormonogens") Nalpha-glycyl-glycyl-lysine-vasopressin, Nalpha-glycyl-glycyl-glycyl-arginine-vasopressin and Nalpha-D-valyl-lysine-vasopressin, b) "carba" modifications desamino-carba6-arginine-vasopressin, desamino-carba6-D-arginine8-vasopressin, desamino-carba6-ornithine8-vasopressin, desamino-dicarba-arginine-vasopressin and c) other steric alterations - desamino-D-arginine8-vasopressin and desamino-N-methylarginine8-vasopressin. Sub-pressor doses of lysine-vasopressin were followed by marked reductions in gut and uterus blood flows which reached a peak at 10 min. and had completely receded by 60 min. The presence of steric alterations in the C-terminal tripeptide of the molecule- D-arginine or N-methylarginine in sequence position 8 - practically completely eliminated vascular activity. The same was true for Nalpha-D-valyl-lysine-vasopressin. None of the latter three analogues showed any inhibitor properties to the action of lysine-vasopressin. The two hormonogens (triglycyl N-terminal extensions) had to be given in doses 10 times greater to obtain a vasoconstrictor effect in gut and uterus equivalent in amplitude to that of a lysine-vasopressin, but this effect was still present to the same degree 2 h later with the hormonogen of lysine-vasopressin, and was only starting to return to baseline values at the same time with the arginine-vasopressin hormonogen. The vascular potency of both mono-carba L-analogues was higher than that of lysine-vasopressin, and the effect was as prolonged as with the hormonogens. The dicarba analogue also showed a prolonged action, but with much reduced potency. No significant changes in renal or myocardial blood flows were observed at all. Molecular features of vasopressin smooth muscle activity were discussed, and a receptor model was proposed. It was suggested that the -S-S-, -CH2CH2-bridges in the above analogues are not directly bound in the peptide-receptor complex and constitute the limiting factor determining complex duration, or persistence of the active peptide in the "receptor compartment". These results provide an experimental basis for possible clinical application of triglycyl-vasopressin and carba-vasopressin in bleeding from both gut and uterus and for induction of menstruation.
Contranidatory activity of early luteal phase administration of mifepristone in the rhesus monkey. (1993)

The contranidatory action of mifepristone (RU 486) given as a single application at different dosages to mated rhesus monkeys (Macaca mulatta) on second day after ovulation has been examined in the present study. In group 1, monkeys (n = 3) received only vehicle (benzyl benzoate: olive oil, 1:4, v/v) and were treated as controls. In group 2 monkeys (n = 4), RU 486 was given by gavage at 10 mg/kg in group 4 (n = 5). The patterns of cycles and profiles of serum estrogen and progesterone were monitored for assessing the occurrence of implantation and pregnancy. At a single dose of 10 mg/kg, RU 486 was found to be ineffective in preventing nidation, resulting pregnancy in three females out of four treated monkeys. Similarly, an s.c. administration of 1 mg/kg could provide pregnancy protection in two of the four treated monkeys. In these monkeys, however, the menstrual cycle characteristics were not affected as compared to pretreatment cycles. Interestingly, the administration of 2 mg/kg, s.c., RU 486 could provide a hundred percent pregnancy protection in mated monkeys, and there was no significant changes in the pattern of menstrual cycle characteristics. It appears that an early post-ovulatory administration of RU 486 may be successfully used in human as an effective once-a-month, early luteal phase contranidatory agent.
Autonomous Visual Self-localization in Completely Unknown Environment using Evolving Fuzzy Rule-based Classifier (2007)

A novel approach to visual self-localization in completely unknown environment with a fully unsupervised and computationally efficient algorithm is proposed in this paper. It is based on the recently developed evolving fuzzy classifier (eClass). The problem of self localization and landmark recognition is of extreme importance for designing efficient and flexible land-based autonomous uninhabited vehicles (AUV). The availability of global coordinates, a GPS link, and unrestricted communication is often compromised by a number of factors, such as interference, weather, and mission objectives. The ability to self-localize and recognize landmarks is vital in such cases for an AUV to survive and function effectively. The self-organizing classifier (eClass) is designed by automatic labeling and grouping the landmarks that are detected in real-time based on the image data (video stream grabbed by the camera mounted on the mobile robot, AUV). The proposed approach makes possible autonomous joint landmark detection and recognition without the use of absolute coordinates, any communication link or any pre-training. The proposed algorithm is recursive, non-iterative, one pass and thus computationally inexpensive and suitable for real-time applications. A set of new formulae for on-line data normalization of the data are introduced in the paper. Real-life tests has been carried out in outdoor environment at the Lancaster University campus using Pioneer3 DX mobile robots equipped with a pan-tilt zoom camera and an on-board PC. The results illustrate the viability and flexibility of the proposed approach. Further investigations will be directed towards teams of mobile robots (AUV) performing a task in completely unknown environment