gebhart/patrepeval huggingface dataset:
Multiplexed Automated DNA Sequencing Directly from Single Bacterial Colonies (1999)

Sample preparation has been one of the major bottlenecks for large-scale DNA sequencing projects in terms of time and cost. To improve sample throughput and to integrate the front-end tasks to capillary-array DNA sequencers, protocols for directly sequencing the plasmids from a single bacterial colony in fused-silica capillaries were developed. After the colony is picked, lysis is accomplished in situ in the plastic sample tube using either a thermocycler or a heating block. Upon heating, the plasmids are released while chromosomal DNA and membrane proteins are denatured and precipitate to the bottom of the tube. After adding enzyme and Sanger reagents, the resulting solution was aspirated into the reaction capillaries by a syringe pump, and cycle sequencing was initiated. No deleterious effect upon the reaction efficiency, the on-line purification system, or the capillary electrophoresis separation was observed, even though the crude lysate was used as the template. Multiplexed online DNA sequencing data from 8 parallel channels allowed base calling up to 620 bp with an accuracy of 98%. The entire system can be automatically regenerated for repeated operation. By the marriage of colony sequencing with the capillary array sequencer, both the front end and the back end of DNA sequencing are combined in a miniaturized format. This protocol will ultimately reduce the cost of sequencing to well below current levels.
Chiral compounds of essential oils. Part XII. Authenticity control of rose oils, using enantioselective multidimensional gas chromatography (1992)

Abstract cis / trans Rose oxides, linalol, citronellol and carvone are widespread natural fragrance compounds and well known among the genuine constituents of rose oils. Using enantioselective multidimensional gas chromatography (enantio‐MDGC) and the column combination Carbowax 20 M/heptakis(2,3‐di‐ O ‐acetyl‐6‐ O ‐ tert ‐butyldimethylsilyl)‐β‐cyclodextrin in OV 1701‐vi the direct and simultaneous stereoanalysis of these chiral fragrance compounds is achieved. The method is applied to the chirality evaluation of cis / trans rose oxides, linalol and citronellol from authentic Bulgarian and Turkish rose oils as well as commercially available rose oils. The enantiomeric ratios of genuine constituents from rose oils are discussed as indicators of naturalness.
Carbon nanotube-penetrated mesoporous V<sub>2</sub>O<sub>5</sub> microspheres as high-performance cathode materials for lithium-ion batteries (2014)

A three-dimensional nanoarchitecture consisting of mesoporous V<sub>2</sub>O<sub>5</sub> and penetrating CNTs was synthesized for high-performance lithium-ion batteries.
Growth and characterization of doped ZrO2 and CeO2 films deposited by bias sputtering (1977)

The deposition rate, chemistry, microstructure, and optical and electrical characteristics of rf sputter deposited Y2O3-doped ZrO2 films have been investigated as a function of applied substrate bias VS. Some preliminary results on Y2O3-doped CeO2 are included. All films were deposited in Ar at a pressure of 20 mTorr (2.67 Pa). The deposition rate of both materials initially increased with VS and then decreased as VS/VT exceeded ∠0.08, where VT is the target voltage. In the doped ZrO2 films, the O/Zr ratio, microstructure, and ionic conductivity activation energy were dependent upon VS. For VT=−500 V, deposited films were approximately stoichiometric at VS?−70 V, while films grown at −VS&lt;60 or ≳80 V were oxygen deficient. Films grown at −VS?40 V had a very columnar microstructure, while films deposited with −VS≳60 V appeared quite dense with little structure visible in fracture cross-section electron micrographs. Electrical measurements indicated ionic-transference numbers within 1% of unity at temperatures T?180°C. Linear Arrhenius plots of measured film resistance versus T in the range 150–550°C yielded activation energies in the range 0.9–1.15 eV and indicated a single-conduction mechanism. All doped ZrO2 and CeO2 films exhibited high optical transmission over most of the visible and near-infrared spectrum. The refractive index decreased with increasing oxygen content and agreed reasonably well with published values for pure ZrO2 films.
Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120 (2006)

During natural HIV infection, an array of host receptors are thought to influence virus attachment and the kinetics of infection. In this study, to probe the interactions of HIV envelope (Env) with various receptors, we assessed the inhibitory properties of various anti-Env monoclonal antibodies (mAbs) in binding assays. To assist in detecting Env in attachment assays, we generated Fc fusions of full-length wild-type gp120 and several variable loop-deleted gp120s. Through investigation of the inhibition of Env binding to cell lines expressing CD4, CCR5, DC-SIGN, syndecans or combinations thereof, we found that the broadly neutralizing mAb, 2G12, directed to a unique carbohydrate epitope of gp120, inhibited Env-CCR5 binding, partially inhibited Env-DC-SIGN binding, but had no effect on Env-syndecan association. Furthermore, 2G12 inhibited Env attachment to primary monocyte-derived dendritic cells, that expressed CD4 and CCR5 primary HIV receptors, as well as DC-SIGN, and suggested that the dual activities of 2G12 could be valuable in vivo for inhibiting initial virus dissemination and propagation.
Identification of Esters of Dibasic Acids by the Use of Ethanolamine (1948)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIdentification of Esters of Dibasic Acids by the Use of EthanolamineWilliam H. Rauscher and William H. ClarkCite this: J. Am. Chem. Soc. 1948, 70, 1, 438Publication Date (Print):January 1, 1948Publication History Published online1 May 2002Published inissue 1 January 1948https://pubs.acs.org/doi/10.1021/ja01181a534https://doi.org/10.1021/ja01181a534research-articleACS PublicationsRequest reuse permissionsArticle Views144Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A first functional current excited planar rotational magnetic micromotor (2002)

Complete integration and successful testing of a planar rotational magnetic micromotor have been demonstrated. The configuration is that of a three-phase variable reluctance stepping motor with 6 stator poles and 4 rotor poles. Stator and rotor heights of up to 300 mu m are available and rotor diameters of 285 mu m and 423 mu m were tested. Maximum rotational speeds which have been achieved with open loop excitation exceed 30000 rpm and show no change with operation in vacuum. After testing to more than 5*10/sup 7/ rotation cycles, deviations from initial operation are not observed. Vertical reluctance forces are used to levitate the rotor up to 50 mu m over the substrate. Position signals are available via integrated photodiodes which allow the rotor speed to be monitored and provide the groundwork for an active closed-loop system.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Synthesis of a Self‐Assembled Hybrid of Ultrananocrystalline Diamond and Carbon Nanotubes (2005)

A self-assembled hybrid of ultrananocrystalline diamond (UNCD) and carbon nanotubes (CNTs) is successfully prepared by their simultaneous growth in an argon-rich Ar/CH4 plasma (see Figure and cover). Control of the relative fractions and configurations of UNCD and CNTs in the hybrid material is demonstrated. This new synthesis pathway enables the development of new nanocarbons with unique mechanical, tribological, and electrochemical properties.
Preparation of Acetoxybenzyl Bromides (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of Acetoxybenzyl BromidesD. L. Fields, J. B. Miller, and D. D. ReynoldsCite this: J. Org. Chem. 1964, 29, 9, 2640–2647Publication Date (Print):September 1, 1964Publication History Published online1 May 2002Published inissue 1 September 1964https://pubs.acs.org/doi/10.1021/jo01032a040https://doi.org/10.1021/jo01032a040research-articleACS PublicationsRequest reuse permissionsArticle Views552Altmetric-Citations32LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Automatic Ingestion Monitor: A Novel Wearable Device for Monitoring of Ingestive Behavior (2014)

Objective monitoring of food intake and ingestive behavior in a free-living environment remains an open problem that has significant implications in study and treatment of obesity and eating disorders. In this paper, a novel wearable sensor system (automatic ingestion monitor, AIM) is presented for objective monitoring of ingestive behavior in free living. The proposed device integrates three sensor modalities that wirelessly interface to a smartphone: a jaw motion sensor, a hand gesture sensor, and an accelerometer. A novel sensor fusion and pattern recognition method was developed for subject-independent food intake recognition. The device and the methodology were validated with data collected from 12 subjects wearing AIM during the course of 24 h in which both the daily activities and the food intake of the subjects were not restricted in any way. Results showed that the system was able to detect food intake with an average accuracy of 89.8%, which suggests that AIM can potentially be used as an instrument to monitor ingestive behavior in free-living individuals.
To be Excluded or to Bind, that is the Question: Arginine Effects on Proteins (2009)

In spite of its wide application to protein refolding, purification, and storage, we have not yet addressed a general solution to the mechanism of the effects of arginine hydrochloride on proteins. To elucidate the mechanism of the effects on proteins, several attempts have been reported. In this review, we would review the attempts from thermodynamic and kinetic viewpoints. Keywords: ArgHCl, guanidine, protein aggregation, preferential interaction, solubility, surface tension
Alternating copolymers of fluorene and donor–acceptor–donor segments designed for miscibility in bulk heterojunction photovoltaics (2009)

A novel copolymer based on alternating fluorene and donor–acceptor–donor segments is reported, together with its photovoltaic properties in blends with fullerene derivatives. The balanced electron and hole mobility of the blends leads to a power-conversion efficiency of 2–3% under solar illumination.
Optimized overload resolution and type matching for Ada (1991)

article Free Access Share on Optimized overload resolution and type matching for Ada Authors: David A. Mundie Incremental Systems Corporation, 319 S. Craig Street, Pittsburgh, PA Incremental Systems Corporation, 319 S. Craig Street, Pittsburgh, PAView Profile , David A. Fisher Incremental Systems Corporation, 319 S. Craig Street, Pittsburgh, PA Incremental Systems Corporation, 319 S. Craig Street, Pittsburgh, PAView Profile Authors Info & Claims ACM SIGAda Ada LettersVolume XIIssue 3Spring 1991 pp 83–90https://doi.org/10.1145/112630.112640Online:01 April 1991Publication History 0citation145DownloadsMetricsTotal Citations0Total Downloads145Last 12 Months1Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Electro-optic light modulator with branched ridge waveguide (1975)

A light modulator based on an electro-optic phase modulation and an optical interference of Mach-Zehnder type has been demonstrated. The modulator is constructed monolithically with a branching ridged optical waveguide on TiO2-diffused LiNbO3. The half-wave voltage is 19 V and the extinction ratio is 34%.
Separation of monoclonal antibody alemtuzumab monomer and dimers using ultrafiltration (2005)

This article examines the feasibility of using ultrafiltration to separate the monomer of the monoclonal antibody alemtuzumab (Campath or Campath-1H) from a mixture of dimer and higher-order oligomers (collectively called "dimers" here). Using parameter scanning ultrafiltration, we initially assessed the suitability of the following membranes: 100 kDa and 300 kDa polyethersulfone (PES) membranes, and a 100 kDa polyvinylidene fluoride (PVDF) membrane. A detailed study was then carried out to examine the effects of operating conditions (such as solution pH, ionic strength, stirring speed, and permeate flux) on the separation of the monomer from the dimers using 300 kDa PES and 100 kDa PVDF membranes. Results of the experiments carried out in the carrier phase ultrafiltration (CPUF) mode indicate that the size-based protein-protein separation critically depends on the membrane used as well as the system hydrodynamics. The separation of the monoclonal antibody monomer and dimers using 100 kDa PVDF membranes in the diafiltration mode was also examined. Experimental results demonstrate that under suitable conditions, it is feasible to obtain the alemtuzumab monomer with a purity of more than 93% and a yield of more than 85% (from a mixture of 75% monomer and 25% dimers, which is the typical composition obtained after affinity chromatography). Simulation study indicates that this could be further improved to a purity of more than 96% and a monomer yield of more than 96% by increasing the selectivity of separation or by employing a two-stage diafiltration process.
In Vivo Bladder Cancer Diagnosis by High-Volume Raman Spectroscopy (2010)

We studied the feasibility of Raman spectroscopy for the diagnosis of bladder cancer in vivo. Since the invasion stage is crucial for the treatment choice, a high-volume based Raman probe was used to investigate the potential of determining the invasiveness of bladder cancer. High quality spectra were obtained from suspicious and nonsuspicious bladder locations during the procedure of transurethral resection of bladder tumors (TURBT) with collection times of 1-5 s. Multivariate analysis was used to generate the classification models. The algorithm was able to distinguish bladder cancer from normal bladder locations with a sensitivity of 85% and a specificity of 79%. The Raman spectra of bladder cancer stages showed a gradual increase in the intensity of specific amino acid peaks and, most likely, an increase in the intensity of DNA peaks.
The EHR Paradox (2005)

Thomas D. East, Ph.D., is the chief information officer of the Alaska Native Medical Center, Anchorage, Alaska.
A modified Hopfield network for two-dimensional module placement (2002)

The problem of two-dimensional module placement is solved using a Hopfield network with the objective of minimizing total wire-length. The network is modified to include quartic terms in its energy functional by making the interconnection matrix dependent on the state of the network. This modification decomposes the two-dimensional problem into two coupled one-dimensional problems. A hierarchical approach based on min-cut placement is used to make the network size grow linearly with the number of modules. Computer simulations of the network show that it is capable of finding good solutions that are competitive with well-known heuristic algorithms, particularly when a probabilistic update rule is used for the neurons to avoid local minima. The network can be easily modified to accommodate hard placement of nodules, unequal module sizes, and the constraints of I/O (input/output) pad connections.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Electron Spin Resonance of α- and β-Cobalt Phthalocyanine<sup>1</sup><sub>a</sub> (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectron Spin Resonance of α- and β-Cobalt Phthalocyanine1aJacques M. Assour and Walter K. KahnCite this: J. Am. Chem. Soc. 1965, 87, 2, 207–212Publication Date (Print):January 1, 1965Publication History Published online1 May 2002Published inissue 1 January 1965https://pubs.acs.org/doi/10.1021/ja01080a013https://doi.org/10.1021/ja01080a013research-articleACS PublicationsRequest reuse permissionsArticle Views667Altmetric-Citations123LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
High-Strength Concrete Columns under Simulated Earthquake Loading (1997)

First Name is required invalid characters Last Name is required invalid characters Email Address is required Invalid Email Address Invalid Email Address
<i>I</i> <i>n</i> <i>s</i> <i>i</i> <i>t</i> <i>u</i> formation of superconducting YBa2Cu3O7−<i>x</i> thin films using pure ozone vapor oxidation (1988)

Superconducting YBa2Cu3O7−x thin films have been prepared by coevaporation using an ozone vapor jet as an oxygen source. Films exhibiting zero resistance at 82 K have been fabricated in situ under high vacuum conditions using substrate temperatures of 700 °C without a post-evaporation anneal in oxygen. This process has implications for in situ measurements of fully superconducting surfaces using a variety of probes as well as for the fabrication of devices and structures whose properties are dependent on surfaces and interfaces.
Respiratory syncytial virus, an ongoing medical dilemma: an expert commentary on respiratory syncytial virus prophylactic and therapeutic pharmaceuticals currently in clinical trials (2015)

As the most important viral cause of severe respiratory disease in infants and increasing recognition as important in the elderly and immunocompromised, respiratory syncytial virus (RSV) is responsible for a massive health burden worldwide. Prophylactic antibodies were successfully developed against RSV. However, their use is restricted to a small group of infants considered at high risk of severe RSV disease. There is still no specific therapeutics or vaccines to combat RSV. As such, it remains a major unmet medical need for most individuals. The World Health Organisations International Clinical Trials Registry Platform (WHO ICTRP) and PubMed were used to identify and review all RSV vaccine, prophylactic and therapeutic candidates currently in clinical trials. This review presents an expert commentary on all RSV-specific prophylactic and therapeutic candidates that have entered clinical trials since 2008.
Exposure of lithium batteries to external hydrostatic pressure (2006)

A battery containing 294 cells in the form of 7 modules of 42 DD lithium / sulfuryl chloride cells in a 7S6P configuration exploded during pigging of a gas pipeline in April 1999. The gas pressure in the pipeline was ca 160 Bar. It is likely that the explosion was caused by a leak in the battery container. This paper describes the behavior of the 30 Ah lithium / sulfuryl chloride DD cell (CSC93) under hydrostatic compression (in water and in inert gas), the effect of state charge and the effect of potting
Synthesis of Nano Calcium Hydroxide in Aqueous Medium (2015)

The present work reports a simple, inexpensive method for synthesis of calcium hydroxide [Ca( OH ) 2 ] nanoparticles ( CHNP s). The method involves chemical precipitation (CP) in aqueous medium at room temperature. Calcium nitrate dihydrate [Ca( NO 3 ) 2 .2H 2 O] and sodium hydroxide were used as precursors. The CHNP s were characterized by Fourier transform infrared (FTIR) spectroscopy, X‐ray diffraction (XRD), Rietveld analysis, field‐emission scanning electron microscopy (FE‐SEM) and transmission electron microscopy (TEM), BET surface area evaluation as well as particle size distribution analysis techniques. The results confirmed the synthesis of CHNP s as the major phase. The CHNP s exhibited an average size of about 350 nm. In addition, some calcite phase formed due to the inevitable carbonation process. A very minor amount of aragonite phase was also present. A schematically developed new qualitative model is proposed to explain the genesis and subsequent evolution of the various phases at the nanoscale. The model helps to identify the rate‐controlling step. It also highlights the implication of reaction kinetics control in synthesis of predesigned nanophase assembly.
Single frequency estimation with non-uniform sampling (2002)

A new frame structure is proposed for pilot symbol assisted modulation (PSAM) systems which includes a small number of known pilots at the start of each frame. This facilitates the estimation of large frequency offsets without aliasing. Cramer Rao lower bounds and the exact ML estimates are derived for AWGN and Rayleigh fading channels. Finally, simulations show that the ML estimator has much better performance than existing reduced complexity estimators when applied to this sparse observation model.