gebhart/patrepeval huggingface dataset:
Lag1p and Lac1p Are Essential for the Acyl-CoA–dependent Ceramide Synthase Reaction in<i>Saccharomyces cerevisae</i> (2001)

Lag1p and Lac1p are two homologous transmembrane proteins of the endoplasmic reticulum in Saccharomyces cerevisiae. Homologous genes have been found in a wide variety of eukaryotes. In yeast, both genes, LAC1 and LAG1, are required for efficient endoplasmic reticulum-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins. In this study, we show that lag1 Delta lac1 Delta cells have reduced sphingolipid levels due to a block of the fumonisin B1-sensitive and acyl-CoA-dependent ceramide synthase reaction. The sphingolipid synthesis defect in lag1 Delta lac1 Delta cells can be partially corrected by overexpression of YPC1 or YDC1, encoding ceramidases that have been reported to have acyl-CoA-independent ceramide synthesis activity. Quadruple mutant cells (lag1 Delta lac1 Delta ypc1 Delta ydc1 Delta) do not make any sphingolipids, but are still viable probably because they produce novel lipids. Moreover, lag1 Delta lac1 Delta cells are resistant to aureobasidin A, an inhibitor of the inositolphosphorylceramide synthase, suggesting that aureobasidin A may be toxic because it leads to increased ceramide levels. Based on these data, LAG1 and LAC1 are the first genes to be identified that are required for the fumonisin B1-sensitive and acyl-CoA-dependent ceramide synthase reaction.
EXPERIMENTAL INFECTION OF MULE DEER WITH Parelaphostrongylus tenuis (1980)

Six adult and three fawn mule deer (Odocoileus hemionus) were experimentally infected with a range of 75-100 infective larvae of Parelaphostrongylus tenuis. Five of the six adult deer developed clinical signs of neurologic disease that terminated in paralysis between 35 and 80 days. The sixth deer developed slight signs of neurologic disease for 10 days, but recovered. All three mule deer fawns developed neurologic disease. Adult meningeal worms were recovered from the subdural space of the spinal cord of two fawns. Eggs were observed on the cranial dura mater of one of these fawns, indicating that P. tenuis can complete its life cycle provided mule deer can survive the damage resulting from the infection. Neither eggs nor larvae of P. tenuis were recovered from the feces or lungs of infected mule deer. Clinical signs and histologic lesions observed in experimentally infected mule deer resembled those reported in infected moose (Alces alces americana). Two critical periods were apparent in mule deer infected with P. tenuis: nematode migration through the spinal neural parenchyma, and penetration of the adult nematodes into the cranial neural parenchyma. While most adult deer were unable to survive the first critical period, fawns survived the first but succumbed to infection during the second critical period.
Brightening the Mind: The Impact of Practicing Gratitude on Focus and Resilience in Learning (2016)

A growing body of groundbreaking research shows that gratitude has the power to heal, energize, and transform lives by enhancing people psychologically, spiritually, physically, and cognitively. This study contributes to the study of gratitude by exploring its impact on focus and resilience in learning. Specifically, this study examines the impact that practicing gratitude has on college students’ ability to focus in class and remain resilient in the face of difficulties while learning.
CORROSION RELIABILITY PREDICTION: LONG TERM NUCLEAR WASTE STORAGE IN YUCCA MOUNTAIN (2005)

The US. Department of Energy has proposed the disposal of high level nuclear waste from commercial and defense reactors in a mined geologic repository under Yucca Mountain, Nevada. The waste will be stored in metallic canisters. The barrier against corrosion will be an Alloy 22 canister and a Ti Grade 7 drip shield. Both of these materials are extremely corrosion resistant. The environment inside Yucca Mountain is relatively benign, but the long time period over which these materials must resist penetration makes corrosion a concern. This paper presents a background of the corrosion issues and shows some recent results regarding measurements of localized corrosion under thin aqueous layers and layers that simulate wet dust deposits.
Constructing regions for small area analysis: material deprivation and colorectal cancer (1994)

We conclude that statistical tools can be employed to provide regions that meet the criteria for small area analysis of health data and the analyst does not have to be tied to large administrative units such as wards. There are some benefits to executing this work within a Geographic Informative System. The method should be of interest to those concerned with health service delivery and the identification of 'problem regions'.
Enzymatic Development of the Human Fetus: The Isozymes of Glutamic Oxaloacetic Transaminase in Developing Human Fetal Tissues (1967)

Summary Studies on GOT and its isozyme during development of the human fetus were undertaken. Total GOT activity was found to show more striking increases after birth in the heart, liver as well as brain, than during the fetal life. In the heart, increased activity of total GOT after birth resulted from an increase in GOTm rather than from GOTs. In the liver, increase in total GOT activity during development resulted from an increase in the activity of both isozymes, but increases due to GOTm were more predominant. In the brain, increase in activity of both GOTm and GOTs were found to be similar, especially during development after birth. Low activity of GOT during fetal life was found to be attributable to a lack of the protein portion of the transaminase.
Effect of insulin on protein phosphatase 2A expression in muscle in type 2 diabetes (2002)

Down-regulation of PP2A-C alpha expression by insulin in skeletal muscle seems to be associated with a normal insulin action on glucose storage, glucose and lipid oxidation. Impaired down-regulation of PP2A-C alpha expression by insulin may be a marker for insulin resistance and contribute to the pathogenesis of type 2 diabetes.
Subungual Bacteria of the Hand: Contribution to the Glove Juice Test; Efficacy of Antimicrobial Detergents (1989)

J. J. Leyden, K. J. McGinley, S. G. Kates, K. B. Myung, Subungual Bacteria of the Hand: Contribution to the Glove Juice Test; Efficacy of Antimicrobial Detergents, Infection Control and Hospital Epidemiology, Vol. 10, No. 10 (Oct., 1989), pp. 451-454
Short Communication: Identification and characterization of multiple splicing forms of bovine prochymosin mRNA (2002)

Bovine prochymosin (bPC) is an inactive precursor of the milk clotting enzyme chymosin (EC 3.4.23.4), that is present in the abomasum of suckling calves. We investigated the pattern of bPC mRNA expression in the calf stomach tissues by RT-PCR assay and sequence analysis of cloned RT-PCR products. We identified multiple isoforms appearing due to alternative splicing of bPC mRNA. Alternative mRNA forms were generated by skipping one to four full exons within the bPC gene. Various splicing events resulted in seven bPC transcripts, which are 99, 114, 213, 237, 336, 351 and 450 nucleotides shorter compared to full-length mRNA. Analysis of amino acid sequences deduced from alternatively spliced mRNA sequences showed no amino acid transversions and no protein reading frame shift for any splice forms.
Robust, low cost indoor positioning using magnetic resonant coupling (2012)

We describe the design, implementation, and evaluation of an indoor positioning system based on resonant magnetic coupling. The system has an accuracy of less than 1 m2 and, because of the underlying physical principle, is robust with respect to disturbances such as people moving around or changes in room configuration. It consists of 16x16x16 cm transmitter coils, each able to cover an area of up to 50 m2, and provides location information to an arbitrary number of mobile receivers with an update rate of up to 30Hz. We evaluate the actual accuracy of the positioning with a robotic arm and show quantitatively that even large metallic objects have little effect on the signal. We then present an elaborate study of the performance of our system for the recognition of abstract locations such as "at the table", "in front of a cabinet". It comprises four different sites with a total of 100 individual locations some as little as 50 cm apart.
Annie Denton Cridge (2013) Los derechos de los hombres y otras utopías. (2013)

Annie Denton Cridge (2013) Los derechos de los hombres y otras utopias. Edicion y Traduccion de Isabel Alquezar Artal y Berta Lazaro Martinez, Logrono, Sinindice. 149pp. ISBN 978-84-940896-3-3
ENHANCED PLATELET AGGREGATION IN CHRONIC RENAL FAILURE PATIENTS RECEIVING HEMODIALYSIS TREATMENT (1977)

<p>We describe the preparation and solution properties of Janus micelles, i.e., non-centrosymmetric nanoparticles with compartmentalized shells, via co-assembly of two fully water-soluble block copolymers. They consist of a mixed core of poly(N-methyl-2-vinyl pyridinium iodide) (P2MVP) and poly(acrylic acid) (PAA), and a shell segregated into two sides, consisting of poly(ethylene oxide) (PEO) or poly(acryl amide) PAAm. These Janus particles form spontaneously and reversibly, i.e., association, dissocation, and reassociation can be carefully controlled via parameters, such as polymer mixing fraction, solution pH, and ionic strength. Dynamic (polarized and depolarized) and static light scattering, cryogenic transmission electron microscopy, small angle neutron and X-ray scattering, and two-dimensional nuclear magnetic resonance spectroscopy are used to monitor the micelle formation and to characterize the micellar structure. The Janus particles were found to be ellipsoidal, with a cigar-like overall shape and a disc-like core. This peculiar morphology is driven by the delicate interplay between two opposing forces: an attraction between the oppositely charged core blocks and a subtle repulsion between the water-soluble, neutral corona blocks.</p>
Magnetic Pulses Elevate Nociceptive Thresholds: Comparisons with Opiate Receptor Compounds in Normal and Seizure-Induced Brain-Damaged Rats (1994)

Normal rats or rats with seizure-induced limbic-thalamic damage were given one of five treatments: control, saline injections, morphine (4 mg/ kg) injections, naloxone (10 mg/kg) injections, or magnetic pulses, on 3 successive days. Flinch thresholds to electric shock were determined before the treatments and 20 and 40 min following the treatments on each of the 3 days. The results indicated that the magnetic pulsed fields (1 s of a 5 × 10 6 T burst firing pattern every 4 s for 20 min) elicited a more prolonged and larger analgesic effect than the other treatments; the effect size was sufficient to be of potential clinical relevance. The characteristics of the magnetic treatment effect suggest there is a release of endogenous analgesics whose half-life is greater than experimental dosages of morphine.
The <i>Drosophila micropia</i> Retrotransposon Encodes a Testis-Specific Antisense RNA Complementary to Reverse Transcriptase (1994)

The micropia transposable element of Drosophila hydei is a long terminal repeat-containing retrotransposon present in both the autosomes and the Y chromosome. micropia expression gives rise to a complex set of sense and antisense RNAs transcribed primarily during spermatogenesis. The most abundant sense RNAs constitute an assortment of heterogeneous high-molecular-weight transcripts expressed as constituents of the Y-chromosomal lampbrush loops of primary spermatocytes. In addition, micropia encodes a full-length RNA that extends between the two long terminal repeats of the element. The major 1.0-kb antisense RNA characterized is complementary to the reverse transcriptase and RNase H coding regions of micropia. It is expressed from a testis-specific promoter during the primary spermatocyte stages and is detectable until spermatid elongation stages. Sequence comparison of this promoter with the 5' region of other testis-specific genes allows the conception of a conserved sequence that is responsible for this pattern of expression. A 284-bp fragment containing this sequence is able to drive testis-specific expression of the Escherichia coli lacZ gene in Drosophila melanogaster. This sequence is conserved in the micropia elements present in other Drosophila species that also encode an antisense RNA. The evolutionary conservation of micropia antisense RNA expression and the sequences responsible for its testis-specific transcription suggests a role for this antisense RNA in the control of germ line expression of the full-length transcript or transposon-encoded proteins.
Design of photonic structures for the enhancement of the light guiding efficiency of fluorescent concentrators (2008)

Photonic structures can be used to eliminate the main loss mechanism in fluorescent concentrators. Simulation routines have been established to investigate the optical characteristic of different photonic crystals. Especially two kinds of structures with an appropriate characteristic have been examined closely. The first is the rugate filter, a one-dimensional photonic structure. In the rugate filter the refractive index is varied sinusoidally over the thickness of the filter. The second is the opal, a three-dimensional photonic crystals made of spheres that are arranged in a self organization process. Filters from these structures have been designed and optimized for the application and fluorescent concentrators and have been optimized. Additional aspects of the structures like angular effects have been examined.
Photodynamic therapy with verteporfin for corneal neovascularization (2012)

Photodynamic therapy with verteporfin was effective for the treatment of corneal neovascularization in the majority of the cases in this study.
Optimizing F-measures by Cost-sensitive Classification (2014)

We present a theoretical analysis of F-measures for binary, multiclass and multilabel classification. These performance measures are non-linear, but in many scenarios they are pseudo-linear functions of the per-class false negative/false positive rate. Based on this observation, we present a general reduction of F-measure maximization to cost-sensitive classification with unknown costs. We then propose an algorithm with provable guarantees to obtain an approximately optimal classifier for the F-measure by solving a series of cost-sensitive classification problems. The strength of our analysis is to be valid on any dataset and any class of classifiers, extending the existing theoretical results on F-measures, which are asymptotic in nature. We present numerical experiments to illustrate the relative importance of cost asymmetry and thresholding when learning linear classifiers on various F-measure optimization tasks.
Granulocyte-Colony Stimulating Factor Mobilizing Autologous Peripheral Blood Stem Cells Transplantation to Form Cardiac Myoid Cells and Angiogenesis (2007)

Objective To study autologous peripheral blood stem cells (PBSCs) transplantation for cardiac myoid cells formation and angionesis after recombinant human granulocyte-colony stimulating factor (rhG-CSF) mobilizing. Methods The 60 white Japanese big-ear rabbits were divided into 3 groups, i.e. transplantation (T) mobiliztor (M) and control (C) groups, each group with 20 rabbits. Myocardial infarction (MI) model was developed by ligating the anterior descending coronary artery. G-CSFs were given continually for 7 d in T and M groups since 1h after MI model development. Cell suspension which derived from the peripheral blood and labeled with BrdU which prepared 1 week ago were injected into infarction regions and borders, while in C groups only the same doses saline was injected. The survival and differentiation of the implanted cells were detected with histological analyses and capillary densities. Results BrdU positive cells which were taken on immature cardiac myoid cells were observed at infarcted areas in T group, which were detected as the Actin positively. HE stains showed that the structures of infarction regions were deranged in C group, but in T and M groups cell arrangements were arranged regularly. Meanwhile, there were a large amount of neogenesis capillaries. The capillaries densities were respectively (58.2±11.5) and (52.3±6.0) per high-power field in T and M groups, while in C group was (21.6±4.9) (P＜0.01). In C group blue collagen fibers were much mare than T and M group under Masson stains (P＜0.01). In T and M groups the cardiac functions were much better than in the C group at the end of 4 weeks, especially ejection fraction were respectively (65.34±2.54)% and (63.40±2.84) in M and T groups. In C group it was only (50.51±6.47)% (P＜0.01). Conclusion After G-CSP mobilizing the implanted PBSCs may survive and differentiate into cardiac myoid cells in infarcted areas and vicinities, at the same time promote neogenisis and improve cardiac function. It is significant that cell transplantation will treat the cardiac infarction in future.
Biochemical and Pharmacological Characterization of [<sup>3</sup>H]GBR 12935 Binding In Vitro to Rat Striatal Membranes: Labeling of the Dopamine Uptake Complex (1987)

Binding of the selective dopamine (DA) uptake inhibitor [3H]GBR 12935 to rat striatal membranes was characterized biochemically and pharmacologically. [3H]GBR 12935 binding at 0 degree C was reversible and saturable and Scatchard analysis indicated a single binding site with a KD of 5.5 nM and a Bmax of 760 pmol/mg tissue. [3H]GBR 12935 labeled two binding sites. One binding site was identified as the classic DA uptake site, since methylphenidate, cocaine, diclofensine, and Lu 19-005 potently inhibited [3H]GBR 12935 binding to it. Binding to the second site was inhibited by high concentrations of the above compounds. IC50 values for inhibition of [3H]GBR 12935 binding to the DA uptake site were proportional to IC50 values for inhibition of DA uptake. However, substrates of DA uptake, e.g., DA and 1-methyl-4-phenylpyridine, and DA releasers, e.g., the amphetamines, inhibited [3H]GBR 12935 binding less than DA uptake. Rate experiments excluded the possibility that these "weak" inhibitors affected the binding by allosteric coupled binding sites. The second binding site was not a noradrenergic, serotonergic, or GABAergic uptake site. Neither was it a dopaminergic, acetylcholinergic, histaminic, serotonergic, or adrenergic receptor. However, [3H]GBR 12935 was potently displaced from it by disubstituted piperazine derivatives, i.e., flupentixol and piflutixol. DA uptake and the DA uptake binding site of [3H]GBR 12935 were located primarily in the striatum, but the piperazine acceptor site was distributed uniformly throughout the brain. Also only the DA uptake binding site was destroyed by 6-OH-DA. Thus, [3H]GBR 12935 labels the classic DA uptake site in rat striatum and also a piperazine acceptor site. Substrates for DA uptake and releasers of DA inhibited [3H]GBR 12935 binding with low potency, but did not alter the rate constants for [3H]GBR 12935 binding. Therefore inhibitors of DA uptake label the carrier site and prevent the carrier process.
Vanadium tetrahydroborates: preparation and characterization of V(.eta.2-BH4)3(PMe3)2 and the unusual unidentate BH4- complex V(.eta.1-BH4)2(dmpe)2 (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVanadium tetrahydroborates: preparation and characterization of V(.eta.2-BH4)3(PMe3)2 and the unusual unidentate BH4- complex V(.eta.1-BH4)2(dmpe)2James A. Jensen and Gregory S. GirolamiCite this: J. Am. Chem. Soc. 1988, 110, 13, 4450–4451Publication Date (Print):June 1, 1988Publication History Published online1 May 2002Published inissue 1 June 1988https://pubs.acs.org/doi/10.1021/ja00221a076https://doi.org/10.1021/ja00221a076research-articleACS PublicationsRequest reuse permissionsArticle Views165Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Arabidopsis MutS Homologs -- AtMSH2, AtMSH3, AtMSH6, and a Novel AtMSH7 -- Form Three Distinct Protein Heterodimers with Different Specificities for Mismatched DNA (2000)

Kevin M. Culligan, John B. Hays, Arabidopsis MutS Homologs -- AtMSH2, AtMSH3, AtMSH6, and a Novel AtMSH7 -- Form Three Distinct Protein Heterodimers with Different Specificities for Mismatched DNA, The Plant Cell, Vol. 12, No. 6 (Jun., 2000), pp. 991-1002
Primary total knee arthroplasty: a comparison of computer-assisted and manual techniques. (2010)

Total knee arthroplasty has proven to be successful in improving a patient's quality of life. Traditional total knee instrumentation aligns 80% of knees within 3 degrees of neutral alignment, leaving 20% with outliers with more than 3 degrees of deformity. Computer-assisted surgery in total knee arthroplasty is a relatively new technique for decreasing these alignment outliers. Database searches from January 1990 through April 2008 were used to review studies comparing manual and computer-assisted techniques in total knee arthroplasty. A multistage assessment was used to ensure the broadest coverage. Potential articles were identified and further examined. Full data extraction was performed on 52 articles using a standardized data collection tool. Analysis of 22 randomized controlled studies showed a clear advantage in terms of alignment for computer-assisted surgery versus manual surgery; however, no studies evaluated the associations between patient characteristics and outcomes or measured functional outcome beyond the degree of malalignment within a short period after the surgery. There is a need for studies that examine functional outcomes more than 1 year postoperatively using standardized assessment tools, especially because malalignment is an intermediate outcome measure that cannot be linked causally in all cases of eventual implant failure.
La gramática en la enseñanza del español como lengua extranjera: ¡Eso está hecho! Aprendiendo el participio (2005)

Podemos definir la palabra Gramática desde dos perspectivas diferentes, aunque relacionadas entre sí: la primera se refiere al saber implícito que cada hablante de una lengua tiene de ésta, y cómo desde su conocimiento interno es capaz de comunicarse de forma competente y comprensible. La segunda visión de gramática es aquélla que se centra en explicar este conocimiento mediante normas, reglas y descripciones que funcionan como guía para el correcto y eficaz uso de la lengua misma.
3′ End labelling of RNA with<sup>32</sup>P suitable for rapid gel sequencing (1978)

A new general method of labelling the 2',3'-diol end of RNA with 32P has been devised suitable for gel sequencing. Poly(A) polymerase (E. coli) is incubated with the RNA and limiting amounts of alpha-32P-ATP. The mono-addition product is then cleaved with periodate and beta-eliminated with aniline, leaving the RNA terminally labelled with 3' 32P-phosphate. When applied to a model compound, tRNAPhe from E. coli, over 28 residues could be read from the 3' end.