gebhart/patrepeval huggingface dataset:
Bile acids and the gut microbiome (2014)

The host and microbiome appear to regulate bile acid pool size. The host produces a large, conjugated hydrophilic bile acid pool, maintained through positive-feedback antagonism of farnesoid X receptor (FXR) in intestine and liver. Members of the microbiome utilize bile acids and their conjugates resulting in agonism of FXR in intestine and liver resulting in a smaller, unconjugated hydrophobic bile acid pool. Hydrophilicity of the bile acid pool is associated with disease states. Reduced bile acid levels in the gut are associated with bacterial overgrowth and inflammation. Diet, antibiotic therapy, and disease states affect the balance of the microbiome-bile acid pool.
W-band monolithic single sideband transceiver for automotive radar applications (2005)

This paper reports the first W-band monolithic single sideband FMCW transceiver with direct digital synthesizer modulation. This heterodyne transceiver improves the system sensitivity over the previously reported homodyne approach. The complete transceiver has better than 12-dB suppression of the image sideband with a nominal LO drive of 9 dBm and is estimated to exhibit a 11-dB noise figure for IF as low as 1 MHz. This MMIC chip was fabricated using TRW production line process and is suitable for automotive radar applications.
DNScup: Strong Cache Consistency Protocol for DNS (2006)

Effective caching in Domain Name System (DNS) is critical to its performance and scalability. Existing DNS only supports weak cache consistency by using the Time-To-Live (TTL) mechanism, which functions reasonably well in normal situations. However, maintaining strong cache consistency in DNS as an indispensable exceptional handling mechanismhas become more and more demanding for three important objectives: (1) to quickly respond and handle exceptional incidents, such as sudden and dramatic Internet failures caused by natural and human disasters, (2) to adapt increasingly frequent changes of IP addresses due to the introduction of dynamic DNS techniques for various stationed and mobile devices on the Internet, and (3) to provide finegrain controls for content delivery services to timely balance server load distributions. With agile adaptation to various exceptional Internet dynamics, strong DNS cache consistency improves the availability and reliability of Internet services. In this paper, we propose a proactive DNS cache update protocol, called DNScup, running as middleware in DNS nameservers, to provide strong cache consistency for DNS. The core of DNScup is a dynamic lease technique to keep track of the local DNS nameservers, whose clients need cache coherence to avoid losing service availability. Based on the DNS Dynamic Update protocol, we have built a DNScup prototype with minor modifications to the current DNS implementation. Our trace-driven simulation and system prototype demonstrate the effectiveness of DNScup and its easy and incremental deployment on the Internet.
Two distinct genes for ADP/ATP translocase are expressed at the mRNA level in adult human liver. (1988)

Several clones hybridizing with a bovine ADP/ATP translocase cDNA were isolated from an adult human liver cDNA library in the vector pEX1. DNA sequence analysis revealed that these clones encode two distinct forms of translocase. In particular, two clones specifying the COOH-end-proximal five-sixths of the protein exhibit a 9% amino acid sequence divergence and totally dissimilar 3' untranslated regions. One of these cDNAs is nearly identical in sequence to an ADP/ATP translocase clone (hp2F1) recently isolated from a human fibroblast cDNA library [Battini, R., Ferrari, S., Kaczmarek, L., Calabretta, B., Chen, S. & Baserga, R. (1987) J. Biol. Chem. 262, 4355-4359], with three amino acid changes and a few differences in the 3' untranslated region. Another clone isolated from the pEX1 library contains a reading frame encoding the remaining, NH2-end-proximal, 37 amino acids of the translocase. This sequence differs significantly (14% amino acid sequence divergence) from the corresponding segment of hp2F1, and the 5' untranslated regions of the two clones are totally dissimilar. RNA transfer hybridization experiments utilizing the clones isolated from the pEX1 library revealed the presence in HeLa cells of three distinct mRNA species. The pattern of hybridization and the sizes of these mRNAs suggest a greater complexity of organization and expression of the ADP/ATP translocase genes in human cells than indicated by the analysis of the cDNA clones.
Joint PHY/MAC Layer Security Design Using ARQ With MRC and Null-Space Independent PAPR-Aware Artificial Noise in SISO Systems (2018)

Automatic-repeat-request (ARQ) as a MAC layer mechanism and artificial noise (AN) as a physical layer mechanism along with the help of maximal ratio combining (MRC), are jointly designed to achieve secrecy. Basically, a special AN, which does not require null-space in the channel, is designed based on the quality of service requirements and the channel condition between the legitimate parties and injected to the data packet. If the same packet is requested by the legitimate receiver (Bob), an AN canceling signal is properly designed and added to the next packet. Then, an AN-free packet is obtained by using MRC process at Bob, while deteriorating the eavesdropper's performance. Furthermore, two simple closed-form expressions of the achievable secure throughput are derived. The first one is given in a closed-form for the case of ARQ scheme without AN, while the second one is given in an upper-bound form for the case of ARQ with AN. Moreover, this paper addresses two critical security-associated problems: 1) the joint design of secrecy, reliability, throughput, delay and the tradeoff among them, and 2) the increase in the peak-to-average power ratio (PAPR) due to the added AN. Finally, the proposed design is extended to OFDM to demonstrate its capability in not only enhancing the secrecy due to the frequency selectivity of the channel, but also in reducing the PAPR and out-of-band emission of OFDM-based waveforms, while maintaining secrecy.
The Nucleophilic Addition/Eiectrophilic Ring Closure Route to Bio-Active Heterocycles (1996)

Abstract: Many bio-active compounds contain one or more saturated or partially saturated heterocycles. Most of these contain one or more stereo­ centres. As a result any attempts to synthesize these compounds must take full account of the stereochemistry. In this article a new approach to the stereoselective synthesis of heterocycles is reviewed. The emphasis is on oxygen-containing heterocycles. The approach involves a sequence of a nucleophilic addition reaction followed by an electrophilic ring closure. Examples are given of the stereoselective synthesis of the heterocyclic subunits of macrolide and polyether antibiotics, y­butyrolactone autoregulators, marine polyether toxins and C-glycosides.
ESGCT and NVGCT Collaborative Congress: The Hague - 23 to 26 October Abstracts (2014)

Cervical cancer is the second most prevalent cancer among women worldwide. The disease develops as a result of infection with high-risk human papillomavirus (HPV) through persistent expression of early proteins E6 and E7 with transforming capacities in cervical epithelial cells. Our group pioneered the application of a replication-defective recombinant viral vector system based on Semliki Forest virus (SFV) for vaccination against cervical cancer. In preclinical studies, we demonstrated that recombinant SFV (rSFV) encoding HPV E6 and E7 (rSFVeE6,7), induces robust HPV-specific cellular immune and memory responses upon intramuscular (i.m.) administration in mice resulting in excellent therapeutic anti-tumour efficacy. Despite the clear potency of the SFV vector system, there are a number of inherent challenges. These include manufacturing costs, shelf-life and anti-vector responses. DNA vaccination is an alternative method with the potential to be inexpensive and safe. In this project, the drawbacks associated with both SFV-based vaccines and DNA vaccines are circumvented with the development of a DNA vaccine based on the SFV replicase (DREP). This next generation vaccine will exploit the advantages of both strategies for the development of an immunotherapeutic response against cervical neoplasia by encoding the fusion protein eE6,7 (DREPeE6,7). Our initial results show that upon intradermal delivery via electroporation of mice with DREP-eE6,7, similar E7-specific responses were elicited as compared to i.m. administration of rSFVeE6,7. These results will be further confirmed for assessment of therapeutic efficacy using a TC-1 tumor model.
Iterative Synthesis of Dual-Shaped Reflectors with Superelliptical Apertures (2006)

Elliptical apertures are used in a wide variety of applications such as earth stations for satellite communications. However, in order to take advantage of the maximum effective area, superelliptical apertures can be used. Most of the available papers on shaping dual-reflectors operate on circular apertures or elliptical surfaces. The scope of this paper is to show the results obtained using a low complexity iterative technique for the synthesis of dual-shaped reflector antennas with superelliptical apertures for satellite communications applications. After the theoretical aspects involved in the synthesis procedure, the results obtained for a practical systems are presented
[Effect of ERK1/2 on low shear stress-induced expression of IL-8 mRNA in human endothelial cells]. (2005)

Fluid shear stress plays an important role in many physiological and pathophysiological processes of the cardiovascular system. It modulates vascular function and structure via stimulating mechanosensitive endothelial cell signal events. Previous studies have identified that the exposure of vascular endothelial cells to fluid mechanical forces can modulate the expressions of many genes, including IL-8 gene. In order to gain an insight into the role of extracellular signal regulated kinase (ERK1/2) signal pathway in the expression of IL-8 mRNA in human umbilical vein endothelial cells (HUVECs) under the stimulation by low shear stress (4.20 dyne/cm2), we employed Western blot to measure phosphorylation of ERK1/2 and used quantitative reversal transcription-polymerase chain reaction (qRT-PCR) to assay the expression of IL-8 mRNA. The results showed: (1) Shear stress could activate ERK1/2 with a rapid, biphasic time course (maximum by 10 min and basal by 2 h); the treatment of HUVECs with Genistein (a highly specific inhibitor of tyrosine protein kinase, TPK) or PD98059 (the inhibitor of mitogen-activated protein/extracellular signal regulated kinase kinase, MEK) culd prevent shear-dependent activation of ERK1/2; (2) When treated with Genistein or PD98059, significant inhibition of IL-8 mRNA expression induced by low shear stress was observed in HUVECs. This in vitro study demonstrates that ERK1/2 plays an important role in IL-8 mRNA expression induced by low shear stress.
NMR investigations of stationary phases (2003)

Abstract An overview of the application of solid‐state and suspended‐state NMR spectroscopy for the characterization of stationary phases is given. Solid‐state 29 Si NMR spectroscopy reveals valuable information about the variety and quantity of the surface species formed in the modification reaction. Solid‐state 13 C NMR spectroscopy gives an insight into the dynamic properties of the immobilized ligands and ligand organization. With the help of 1 H and 13 C suspended‐state NMR spectroscopy, investigations can be performed in the presence of a mobile phase. Thus, the structural and dynamic properties of the stationary phase under real‐life chromatographic conditions can be derived from the NMR investigations. These findings can be directly correlated with the resulting chromatographic separation behaviour.
3D model based gesture acquisition using a single camera (2003)

We present a method for 3D human motion capture using a single camera, without markers and without a priori knowledge on gestures. It is based on registering a 3D articulated model on color images with respect to biomechanical constraints. Gestures regularization is discussed as a way to cope with projection ambiguities. Computation is reduced by registering only the moving parts of the body.
Effect of Nanoclay along with Other Effective Parameters on Gelation Time of Hydro Polymer Gels (2012)

In this work, a hydrogel was prepared by crosslinking of aqueous solutions of sulfonated polyacrylamide/chromium triacetate for the purpose of water shut-off treatment in oil fields. In order to screen the factors that affect the gelation time and investigate their interactions, a 32 run fractional factorial design was used in experimentation with eight factors (pH, CaCl2 concentration, crosslinker/copolymer ratio, NaCl concentration, copolymer concentration and presence or absence of sodium lactate, nanoclay, and thiouria) and one response (gelation time). Furthermore, the analysis of variance (ANOVA) was implemented as a significant tool to evaluate the quality of the quadratic model. The ANOVA results of the developed model showed it was significant with a 99% confidence limit. Among the eight factors, presence of sodium lactate, copolymer concentration, presence of nanoclay, presence of thiouria, and crosslinker/copolymer ratio had, in order, the main effects, and the interactions between sodium lactate and crosslinker/copolymer ratio and between pH and crosslinker/copolymer ratio were highly significant.
An Extension of Phase Correlation-Based Image Registration to Estimate Similarity Transform Using Multiple Polar Fourier Transform (2018)

Image registration is a core technology of many different image processing areas and is widely used in the remote sensing community. The accuracy of image registration largely determines the effect of subsequent applications. In recent years, phase correlation-based image registration has drawn much attention because of its high accuracy and efficiency as well as its robustness to gray difference and even slight changes in content. Many researchers have reported that the phase correlation method can acquire a sub-pixel accuracy of 1 / 10 or even 1 / 100 . However, its performance is acquired only in the case of translation, which limits the scope of the application of the method. However, there are few reports on the estimation of scales and angles based on the phase correlation method. To take advantage of the high accuracy property and other merits of phase correlation-based image registration and extend it to estimate the similarity transform, we proposed a novel algorithm, the Multilayer Polar Fourier Transform (MPFT), which uses a fast and accurate polar Fourier transform with different scaling factors to calculate the log-polar Fourier transform. The structure of the polar grids of MPFT is more similar to the one of the log-polar grid. In particular, for rotation estimation only, the polar grid of MPFT is the calculation grid. To validate its effectiveness and high accuracy in estimating angles and scales, both qualitative and quantitative experiments were carried out. The quantitative experiments included a numerical simulation as well as synthetic and real data experiments. The experimental results showed that the proposed method, MPFT, performs better than the existing phase correlation-based similarity transform estimation methods, the Pseudo-polar Fourier Transform (PPFT) and the Multilayer Fractional Fourier Transform method (MLFFT), and the classical feature-based registration method, Scale-Invariant Feature Transform (SIFT), and its variant, ms-SIFT.
Real-Time Human Detection as an Edge Service Enabled by a Lightweight CNN (2018)

Edge computing allows more computing tasks to take place on the decentralized nodes at the edge of networks. Today many delay sensitive, mission-critical applications can leverage these edge devices to reduce the time delay or even to enable real-time, online decision making thanks to their onsite presence. Human objects detection, behavior recognition and prediction in smart surveillance fall into that category, where a transition of a huge volume of video streaming data can take valuable time and place heavy pressure on communication networks. It is widely recognized that video processing and object detection are computing intensive and too expensive to be handled by resource-limited edge devices. Inspired by the depthwise separable convolution and Single Shot Multi-Box Detector (SSD), a lightweight Convolutional Neural Network (L-CNN) is introduced in this paper. By narrowing down the classifier's searching space to focus on human objects in surveillance video frames, the proposed L-CNN algorithm is able to detect pedestrians with an affordable computation workload to an edge device. A prototype has been implemented on an edge node (Raspberry PI 3) using openCV libraries, and satisfactory performance is achieved using real-world surveillance video streams. The experimental study has validated the design of L-CNN and shown it is a promising approach to computing intensive applications at the edge.
618 Accuracy of measurement of knee kinematics using single-plane fluoroscopic image (2007)

The Japan Sooiety of Meohanioal Engineers 618 X
The effect of antitumor drugs on oxidative stress in B16 and S91 melanoma cells in vitro. (2005)

This study examined the effect of actinomycin-D (AMD), adriamycin (ADR), cisplatin (Cis-Pt), vincristine (VCR), cytosine arabinoside (Ara-C) and dacarbazine (DTIC) on the survival of B16 and S91 mouse melanoma cells in vitro, and on the concentration of thiobarbituric acid reactive substances (TBARS), the content of conjugated dienes (CD), and the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx).B16 and S91 mice melanoma cells were cultivated in culture medium. After the selected drugs had been added to the culture medium, the viability of the melanoma cells was tested using two methods: the cell count in a phase contrast microscope with inverted optics, and the MTT Test.The survival of melanoma cells after incubation with all cytostatic drugs was significantly lower than in the controls. The concentrations of CD and TBARS and the activity of antioxidant enzymes in melanoma cells after incubation with antitumor drugs were higher than in the controls. The highest concentration of CD and TBARS was shown after incubation with ADR. The highest activity of SOD was noticed after incubation with AMD. The highest activity of CAT was found after incubation with AMD in B16 cells and with Cis-Pt in S91 cells. The highest activity of GPx was found after incubation with Ara-C.The changes in antioxidant enzyme activity and the concentration of lipid peroxidation products confirm the participation of reactive oxygen species (ROS) in the cytotoxic action of antitumor drugs.
A Three-Megabase Yeast Artificial Chromosome Contig Spanning the C57BL Mouse <i>Igh</i> Locus (2002)

The mouse Ig H chain (Igh) complex locus is composed of >100 gene segments encoding the variable, diversity, joining, and constant portions of the Ab H chain protein. To advance the characterization of this locus and to identify all the V(H) genes, we have isolated the entire region from C57BL/6 and C57BL/10 as a yeast artificial chromosome contig. The mouse Igh locus extends approximately three megabases and contains at least 134 V(H) genes classified in 15 partially interspersed families. Two non-Igh pseudogenes (Odc-rs8 and Rpl32-rs14) were localized in the distal part of the locus. This physical yeast artificial chromosome map will provide important structure and guidance for the sequencing of this large, complex, and highly repetitive locus.
Interaction of metal layers with polycrystalline Si (1976)

Solid-phase reactions of metal films deposited on 0.5-μm-thick polycrystalline layers of Si grown by chemical vapor deposition at 640 °C were investigated by MeV 4He backscattering spectrometry, glancing angle x-ray diffraction, and SEM observations. For the metals Al, Ag, and Au, which form simple eutectics, heat treatment at temperatures below the eutectic results in erosion of the poly-Si layer and growth of Si crystallites in the metal film. Crystallite formation is observed at T≳550 °C for Ag, T ≳400 °C for Al, and T ≳200 °C for Au films. For the metals Pd, Ni, and Cr, heat treatment results in silicide formation. The same initial silicides (Pd2Si, Ni2Si, and CrSi2), are formed at similar temperatures on single-crystal substrates.
Global Mass Spectrometry and Transcriptomics Array Based Drug Profiling Provides Novel Insight into Glucosamine Induced Endoplasmic Reticulum Stress (2014)

We investigated the molecular effects of glucosamine supplements, a popular and safe alternative to nonsteroidal anti-inflammatory drugs, for decreasing pain, inflammation, and maintaining healthy joints. Numerous studies have reported an array of molecular effects after glucosamine treatment. We questioned whether the differences in the effects observed in previous studies were associated with the focus on a specific subproteome or with the use of specific cell lines or tissues. To address this question, global mass spectrometry- and transcription array-based glucosamine drug profiling was performed on malignant cell lines from different stages of lymphocyte development. We combined global label-free MS-based protein quantitation with an open search for modifications to obtain the best possible proteome coverage. Our data were largely consistent with previous studies in a variety of cellular models. We mainly observed glucosamine induced O-GlcNAcylation/O-GalNAcylation (O-HexNAcylation); however, we also observed global and local changes in acetylation, methylation, and phosphorylation. For example, our data provides two additional examples of "yin-yang" between phosphorylation and O-HexNAcylation. Furthermore, we mapped novel O-HexNAc sites on GLU2B and calnexin. GLU2B and calnexin are known to be located in the endoplasmic reticulum (ER) and involved in protein folding and quality control. The O-HexNAc sites were regulated by glucosamine treatment and correlated with the up-regulation of the ER stress marker GRP78. The occupancy of O-HexNAc on GLU2B and calnexin sites differed between the cytosolic and nuclear fractions with a higher occupancy in the cytosolic fraction. Based on our data we propose the hypothesis that O-HexNAc either inactivates calnexin and/or targets it to the cytosolic fraction. Further, we hypothesize that O-HexNAcylation induced by glucosamine treatment enhances protein trafficking.
Oxidation Resistance of Graphite Powders Coated with Al2O3-Based Oxides. (1999)

Ethyl acetoacetate aluminium diisopropoxide, magnesium acetate tetrahydrate, titanium tetraisopropoxide, zirconium tetra-n-butoxide, tetraethyl orthosilicate and boric acid were used to prepare graphite powders coated with Al2O3-based oxides. The coating effect with Al2O3-based oxides on oxidation resistance of the graphite powders were studied. The oxidation resistance of the graphite powders coated with Al2O3-ZrO2, Al2O3-TiO2, Al2O3-B2O3 and Al2O3-SiO2 was higher than that of the graphite powder coated with Al2O3 monoxide, and the graphite powders coated with 90Al2O3⋅10SiO2 (mass%) showed the highest oxidation resistance. The coating layer of 90Al2O3⋅10SiO2 (mass%) showed the lower true density, higher specific surface area and lower linear shrinkage than those of Al2O3. It is considered that these properties of 90Al2O3⋅10SiO2 (mass%) coated graphite determined the high oxidation resistance of the graphite powders.