gebhart/patrepeval huggingface dataset:
Cyberbullying in Higher Education (2013)

Bullying has extended beyond the schoolyard into online forums in the form of cyberbullying. Cyberbullying is a growing concern due to the effect on its victims. Current studies focus on grades K–12; however, cyberbullying has entered the world of higher education. The focus of this study was to identify the existence of cyberbullying in higher education, reveal the existence of students bullying instructors, and determine its impact. Three hundred forty-six online instructors from the undergraduate, graduate, and doctoral programs at the school of management at a large online university were surveyed. Of the respondents, 33.8 % said they had been cyberbullied by students, 4.4 % of respondents were unsure, and 61.8 % said they had never been cyberbullied by students. Over 60 % of the participants did not know what resources were available or felt that there were no resources available to help them should they encounter cyberbullying by students in the online classroom. Results indicated concerns about reporting cyberbullying, ranging from fear of not getting further teaching opportunities to dealing with it and decreasing the rate of student retention.
Loco signaling pathway in longevity (2011)

Despite the various roles of regulator of G protein signaling (RGS) protein in the G protein signaling pathway that have been defined, the function of RGS has not been characterized in longevity signaling pathways. We found that reduced expression of Loco, a Drosophila RGS protein, resulted in a longer lifespan of flies with stronger resistance to stress, higher MnSOD activity and increased fat content. In contrast, overexpression of the loco gene shortened the fly lifespan significantly, lowered stress resistance and reduced fat content, also indicating that the RGS domain containing GTPase-activating protein (GAP) activity is related to the regulation of longevity. Interestingly, expressional changes of yeast RGS2 and rat RGS14, homologs to the fly Loco, also affected oxidative stress resistance and longevity in the respective species. It is known that Loco inactivates inhibitory Gαi•GTP protein to reduce activity of adenylate cyclase (AC) and RGS14 interacts with activated H-Ras and Raf-1 kinases, which subsequently inhibits ERK phosphorylation. We propose that Loco/RGS14 protein may regulate stress resistance and longevity as an activator in AC-cAMP-PKA pathway and/or as a molecular scaffold that sequesters active Ras and Raf from Ras•GTP-Raf-MEK-ERK signaling pathway. Consistently, our data showed that downregulation of Loco significantly diminishes cAMP amounts and increases p-ERK levels with higher resistance to the oxidative stress.
Generation of Healthy Mice from Gene-Corrected Disease-Specific Induced Pluripotent Stem Cells (2011)

Using the murine model of tyrosinemia type 1 (fumarylacetoacetate hydrolase [FAH] deficiency; FAH⁻/⁻ mice) as a paradigm for orphan disorders, such as hereditary metabolic liver diseases, we evaluated fibroblast-derived FAH⁻/⁻-induced pluripotent stem cells (iPS cells) as targets for gene correction in combination with the tetraploid embryo complementation method. First, after characterizing the FAH⁻/⁻ iPS cell lines, we aggregated FAH⁻/⁻-iPS cells with tetraploid embryos and obtained entirely FAH⁻/⁻-iPS cell-derived mice that were viable and exhibited the phenotype of the founding FAH⁻/⁻ mice. Then, we transduced FAH cDNA into the FAH⁻/⁻-iPS cells using a third-generation lentiviral vector to generate gene-corrected iPS cells. We could not detect any chromosomal alterations in these cells by high-resolution array CGH analysis, and after their aggregation with tetraploid embryos, we obtained fully iPS cell-derived healthy mice with an astonishing high efficiency for full-term development of up to 63.3%. The gene correction was validated functionally by the long-term survival and expansion of FAH-positive cells of these mice after withdrawal of the rescuing drug NTBC (2-(2-nitro-4-fluoromethylbenzoyl)-1,3-cyclohexanedione). Furthermore, our results demonstrate that both a liver-specific promoter (transthyretin, TTR)-driven FAH transgene and a strong viral promoter (from spleen focus-forming virus, SFFV)-driven FAH transgene rescued the FAH-deficiency phenotypes in the mice derived from the respective gene-corrected iPS cells. In conclusion, our data demonstrate that a lentiviral gene repair strategy does not abrogate the full pluripotent potential of fibroblast-derived iPS cells, and genetic manipulation of iPS cells in combination with tetraploid embryo aggregation provides a practical and rapid approach to evaluate the efficacy of gene correction of human diseases in mouse models.
Broadband Underwater Localization of Multiple Sources Using Basis Pursuit De-Noising (2011)

Locating multiple underwater acoustic sources is a problem that can be solved using antenna array beamforming based on the matched field (MF) processing. However, known MF beamforming techniques fail to provide good performance for multiple sources, a high noise power, and/or when the sources are close to each other. This paper proposes an MF technique for solving the localization problem. The proposed technique exploits formulation of the localization problem in terms of sparse representation of a small number of source positions among a much larger number of potential positions. The sparse representation is formulated as the basis pursuit de-noising (BPDN) problem for complex-valued variables. The solution is found as a joint solution to a set of BPDN problems corresponding to the set of source frequencies subject to the joint support. The joint BPDN problem is efficiently solved using the Homotopy approach and coordinate descent search. For further reduction in the complexity, a position grid refinement method is applied. Using simulated and real experimental data, it is shown that the technique can provide accurate source localization for multiple sources. The proposed technique outperforms other MF techniques in resolving sources positioned closely to each other, tolerance to the noise and capability of locating multiple sources.
Incompatibility of linear DNA killer plasmids pGKL1 and pGKL2 from Kluyveromyces lactis with mitochondrial DNA from Saccharomyces cerevisiae (1984)

Two linear killer plasmids (pGKL1 and pGKL2) from Kluyveromyces lactis stably replicated and expressed the killer phenotype in a neutral petite mutant [( rho0]) of Saccharomyces cerevisiae. However, when cytoplasmic components were introduced by cytoduction from a wild-type [( rho+]) strain of S. cerevisiae, the linear plasmids became unstable and were frequently lost from the cytoductant cells during mitosis, giving rise to nonkiller clones. The phenomenon was ascribed to the incompatibility with the introduced S. cerevisiae mitochondrial DNA (mtDNA), because the plasmid stability was restored by [rho0] mutations in the cytoductant cells. Incompatibility with mtDNA was also apparent for the transmission of plasmids into diploid progeny in crosses between killer cells carrying the pGKL plasmids and [rho+] nonkiller cells lacking the plasmids. High-frequency transmission of the plasmids was observed in crosses lacking mtDNA [( rho0] by [rho0] crosses) and in crosses involving mutated mtDNA with large deletions of various regions of mitochondrial genome. In contrast, mutated mtDNA from various mit- mutations also exerted the incompatibility effect on the transmission of plasmids. Double-stranded RNA killer plasmids were stably maintained and transmitted in the presence of wild-type mtDNA and stably coexisted with pGKL killer plasmids in [rho0] cells of S. cerevisiae.
Identification of the First Prokaryotic Collagen Sequence Motif That Mediates Binding to Human Collagen Receptors, Integrins α2β1 and α11β1 (2008)

Many pathogenic bacteria interact with human integrins to enter host cells and to augment host colonization. Group A Streptococcus (GAS) employs molecular mimicry by direct interactions between the cell surface streptococcal collagen-like protein-1 (Scl1) and the human collagen receptor, integrin alpha2beta1. The collagen-like (CL) region of the Scl1 protein mediates integrin-binding, although, the integrin binding motif was not defined. Here, we used molecular cloning and site-directed mutagenesis to identify the GLPGER sequence as the alpha2beta1 and the alpha11beta1 binding motif. Electron microscopy experiments mapped binding sites of the recombinant alpha2-integrin-inserted domain to the GLPGER motif of the recombinant Scl (rScl) protein. rScl proteins and a synthetic peptide harboring the GLPGER motif mediated the attachment of C2C12-alpha2+myoblasts expressing the alpha2beta1 integrin as the sole collagen receptor. The C2C12-alpha11+myoblasts expressing the alpha11beta1 integrin also attached to GLPGER-harboring rScl proteins. Furthermore, the C2C12-alpha11+cells attached to rScl1 more efficiently than C2C12-alpha2+cells, suggesting that the alpha11beta1 integrin may have a higher binding affinity for the GLPGER sequence. Human endothelial cells and dermal fibroblasts adhered to rScl proteins, indicating that multiple cell types may recognize and bind the Scl proteins via their collagen receptors. This work is a stepping stone toward defining the utilization of collagen receptors by microbial collagen-like proteins that are expressed by pathogenic bacteria.
Circuit board inspection using a range camera (1991)

A high-speed, high-accuracy three-dimensional (3-D) range camera that possesses sufficient speed and accuracy to inspect circuit boards in real time is presented. The architecture of the system is briefly described, and the algorithms used to make quantitative presolder measurements, including the orientations and lengths of leads and the locations and shapes of holes, are examined in detail. Since the unit runs in real time and performs sophisticated image analysis functions, considerable emphasis in the algorithm development was placed on the ability to partition tasks between general-purpose computers and array processors.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
DNA Replication and Transcription in Mammalian Mitochondria (2007)

The mitochondrion was originally a free-living prokaryotic organism, which explains the presence of a compact mammalian mitochondrial DNA (mtDNA) in contemporary mammalian cells. The genome encodes for key subunits of the electron transport chain and RNA components needed for mitochondrial translation. Nuclear genes encode the enzyme systems responsible for mtDNA replication and transcription. Several of the key components of these systems are related to proteins replicating and transcribing DNA in bacteriophages. This observation has led to the proposition that some genes required for DNA replication and transcription were acquired together from a phage early in the evolution of the eukaryotic cell, already at the time of the mitochondrial endosymbiosis. Recent years have seen a rapid development in our molecular understanding of these machineries, but many aspects still remain unknown.
Fis Regulates Transcriptional Induction of RpoS in<i>Salmonella enterica</i> (2005)

The sigma factor RpoS is known to regulate at least 60 genes in response to environmental sources of stress or during growth to stationary phase (SP). Accumulation of RpoS relies on integration of multiple genetic controls, including regulation at the levels of transcription, translation, protein stability, and protein activity. Growth to SP in rich medium results in a 30-fold induction of RpoS, although the mechanism of this regulation is not understood. We characterized the activity of promoters serving rpoS in Salmonella enterica serovar Typhimurium and report that regulation of transcription during growth into SP depends on Fis, a DNA-binding protein whose abundance is high during exponential growth and very low in SP. A fis mutant of S. enterica serovar Typhimurium showed a ninefold increase in expression from the major rpoS promoter (PrpoS) during exponential growth, whereas expression during SP was unaffected. Increased transcription from PrpoS in the absence of Fis eliminated the transcriptional induction as cells enter SP. The mutant phenotype can be complemented by wild-type fis carried on a single-copy plasmid. Fis regulation of rpoS requires the presence of a Fis site positioned at -50 with respect to PrpoS, and this site is bound by Fis in vitro. A model is presented in which Fis binding to this site allows repression of rpoS specifically during exponential growth, thus mediating transcriptional regulation of rpoS.
Neuroserpin reduces cerebral infarct volume and protects neurons from ischemia-induced apoptosis (2000)

Neuroserpin, a recently identified inhibitor of tissue-type plasminogen activator (tPA), is primarily localized to neurons within the central nervous system, where it is thought to regulate tPA activity. In the present study neuroserpin expression and its potential therapeutic benefits were examined in a rat model of stroke. Neuroserpin expression increased in neurons surrounding the ischemic core (ischemic penumbra) within 6 hours of occlusion of the middle cerebral artery and remained elevated during the first week after the ischemic insult. Injection of neuroserpin directly into the brain immediately after infarct reduced stroke volume by 64% at 72 hours compared with control animals. In untreated animals both tPA and urokinase-type plasminogen activator (uPA) activity was significantly increased within the region of infarct by 6 hours after reperfusion. Activity of tPA then decreased to control levels by 72 hours, whereas uPA activity continued to rise and was dramatically increased by 72 hours. Both tPA and uPA activity were significantly reduced in neuroserpin-treated animals. Immunohistochemical staining of basement membrane laminin with a monoclonal antibody directed toward a cryptic epitope suggested that proteolysis of the basement membrane occurred as early as 10 minutes after reperfusion and that intracerebral administration of neuroserpin significantly reduced this proteolysis. Neuroserpin also decreased apoptotic cell counts in the ischemic penumbra by more than 50%. Thus, neuroserpin may be a naturally occurring neuroprotective proteinase inhibitor, whose therapeutic administration decreases stroke volume most likely by inhibiting proteinase activity and subsequent apoptosis associated with focal cerebral ischemia/reperfusion. (Blood. 2000;96:569-576)
Approximate Nearest Neighbor Search for a Dataset of Normalized Vectors (2009)

This paper describes a novel algorithm for approximate nearest neighbor searching. For solving this problem especially in high dimensional spaces, one of the best-known algorithm is Locality-Sensitive Hashing (LSH). This paper presents a variant of the LSH algorithm that outperforms previously proposed methods when the dataset consists of vectors normalized to unit length, which is often the case in pattern recognition. The LSH scheme is based on a family of hash functions that preserves the locality of points. This paper points out that for our special case problem we can design efficient hash functions that map a point on the hypersphere into the closest vertex of the randomly rotated regular polytope. The computational analysis confirmed that the proposed method could improve the exponent ρ, the main indicator of the performance of the LSH algorithm. The practical experiments also supported the efficiency of our algorithm both in time and in space.
Light absorption in conical silicon particles (2013)

The problem of the absorption of light by a nanoscale dielectric cone is discussed. A simplified solution based on the analytical Mie theory of scattering and absorption by cylindrical objects is proposed and supported by the experimental observation of sharply localized holes in conical silicon tips after high-fluence irradiation. This study reveals that light couples with tapered objects dominantly at specific locations, where the local radius corresponds to one of the resonant radii of a cylindrical object, as predicted by Mie theory.
N-retinoyl-D-glucosamine, a new retinoic acid agonist, mediates topical retinoid efficacy with no irritation on photoaged skin (2005)

Topical GRA appears to be able to repair photoaged skin damage without any of the irritation caused by topical RA, probably via RAR transactivation activity.
Enrichment of fetal and maternal long cell‐free DNA fragments from maternal plasma following DNA repair (2018)

Plasma DNA repair recovers and enriches long cfDNA molecules of both fetal and maternal origins in maternal plasma.
Test generation techniques for sequential circuits (2002)

The authors present several methods to enhance the performance of sequential test generation algorithms. Among the innovations proposed are a new circuit model, a novel learning technique, new methods to deal with testability measures and a powerful procedure to identify untestable faults. They use an enhanced implementation of the BACK algorithm together with a set of published benchmark circuits to demonstrate the efficiency of the proposed techniques. The results show that the overall performance of the BACK algorithm is greatly improved. For many of the benchmark circuits, test generation time is reduced by more than one order of magnitude.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Advanced Algorithm for Determining Component Spectra Based on Principal Component Analysis (1985)

A method is proposed for determining the component spectra from a set of unknown two-component mixture spectra. This method is considered as an advanced version of the Lawton-Sylvestre self-modeling curve resolution. The disadvantage of the weakness against noise is overcome by the introduction of the concept of filtering; the uncertainty of the solution is removed by the reduction of the band for each curve to a single curve with aid of the concept of entropy minimization. Experimental results with infrared absorption spectra of xylene isomer mixtures and visible absorption spectra of dye mixtures demonstrate the power of this method.
High-density integrated planar lightwave circuits using SiO/sub 2/-GeO/sub 2/ waveguides with a high refractive index difference (1994)

GeO/sub 2/-doped silica waveguides with a high refractive index difference of 1.5% are successfully fabricated on Si substrates. Their propagation loss, measured in 200-cm-long test circuits with a minimum curvature radius of 2 mm, is 0.073 dB/cm. The waveguides are used as high-density integrated planar lightwave circuits in 1/spl times/4 Mach-Zehnder (MZ) type multi/demultiplexers for optical frequency division multiplexing (FDM) transmission systems and in modified MZ type multi/demultiplexers with a ring resonator, which have a compact device size of 15/spl times/50 mm/sup 2/ and a frequency spacing of 10 GHz.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Development of a Non-protein and Lipid Medium Adopted Cell Line forBiopharmaceutical Recombinant Protein Expression (2013)

Recently, many biopharmaceuticals have been developed such as cytokines, growth factors, and antibodies. These recombinant proteins are mostly expressed by CHO cells. However, the culture medium of CHO cells requires the addition of serum, which can contain unknown biological substances such as viruses, or requires the addition of expensive growth factors. To avoid the risks of biological ingredients and to decrease the cost of biopharmaceutical production, we developed a non-protein and lipid medium adopted (NPLAd) CHO cell line using the adapted culture method. Our results indicated that autocrine EGF production and insulin addition are essential for NPLAd CHO cell growth. However, the rate of cell proliferation of NPLAd CHO cells was decreased compared with original CHO-K1 cells. The proliferation of NPLAd CHO cells was improved by GM3 addition, suggesting increased signaling efficiency of autocrine factors. No difference was found in the growth rate between original CHO-K1 and NPLAd CHO cells supplemented with insulin and GM3. The productivity of recombinant protein in NPLAd CHO cells was verified using secreting luciferase reporter system. As a result, luciferase activity in NPLAd CHO cells showed more than three times higher than in the original CHOK1 cells. The results suggested that this cell line could be useful for biopharmaceutical recombinant protein.
A detailed implement and analysis of mpls VPN based on IPSEC (2005)

VPN simply based on multiprotocol label switching (MPLS) has some outstanding security problems in some aspects. Hence, we bring forward a specific and particular security enhanced solution of VPN based on MPLS. The core idea of this scheme is as follows: after considering the advantages of MPLS and IPSec in transfer efficiency and security, import CA certificate management scheme during the implementation of IPSec so as to build a secure and efficient VPN over MPLS core networks. The paper describes the specific steps of enhanced MPLS VPN in details and compares the capacity of different MPLS VPNs with the result of simulation.
The architecture and design of HS-series StorageWorks array controllers (1994)

The HS series of StorageWorks array controllers is a new family of Digital products that includes models for both open systems and systems that use Digital&apos;s proprietary buses. The HS-series controllers combine performance, availability, and reliability in total storage subsystem solutions that use industry-standard storage devices. The architecture and design of StorageWorks array controllers represents a balance between the market requirements and the available technology. The engineering trade-offs led to an innovative design that incorporates product features such as a dual-active controller configurations,
Spatially Ordered Dynamics of the Bacterial Carbon Fixation Machinery (2010)

Cyanobacterial carbon fixation is a major component of the global carbon cycle. This process requires the carboxysome, an organelle-like proteinaceous microcompartment that sequesters the enzymes of carbon fixation from the cytoplasm. Here, fluorescently tagged carboxysomes were found to be spatially ordered in a linear fashion. As a consequence, cells undergoing division evenly segregated carboxysomes in a nonrandom process. Mutation of the cytoskeletal protein ParA specifically disrupted carboxysome order, promoted random carboxysome segregation during cell division, and impaired carbon fixation after disparate partitioning. Thus, cyanobacteria use the cytoskeleton to control the spatial arrangement of carboxysomes and to optimize the metabolic process of carbon fixation.
Smooth Splines Over Irregular Meshes Built From Few Polynomial Pieces of Low Degree (1993)

Smooth splines over irregular meshes built from few polynomial pieces of low degree by
Diffusion Coefficients of Lithium Ions during Intercalation into Graphite Derived from the Simultaneous Measurements and Modeling of Electrochemical Impedance and Potentiostatic Intermittent Titration Characteristics of Thin Graphite Electrodes (1997)

The solid state diffusion of lithium into graphite during electrochemical intercalation processes was investigated using potentiostatic intermittent titration (PITT) and impedance spectroscopy (EIS). The diffusion coefficient (D) as a function of the intercalation level (X) and the electrode potential (E) was calculated on the basis of both methods and gave similar results. The D vs X or E plots were found to be nonmonotonous, with three pronounced minima at the same potentials in which the cyclic voltammetry of these systems shows the peaks related to the phase transition between the lithium−graphite intercalation stages. It was found that the critical diffusion length for these electrodes relates to the graphite particles' dimensions along their basal planes and not to the electrode thickness. The reason for the peaklike dependence of the D on X and E is discussed in light of the nature of the intercalation processes.