gebhart/patrepeval huggingface dataset:
Bryostatin 1 and phorbol ester down-modulate protein kinase C-alpha and -epsilon via the ubiquitin/proteasome pathway in human fibroblasts. (1997)

We evaluated the possibility that distinct proteolytic pathways contribute to the down-regulation of a novel (epsilon) or conventional (alpha) isoform of protein kinase C (PKC) in nonimmortalized human fibroblasts. Inhibitors of calpains and other cysteine proteinases, vesicle trafficking, or lysosomal proteolysis did not affect the down-regulation of PKC-alpha or -epsilon produced by bryostatin 1 (Bryo). Lactacystin (Lacta) and certain terminal aldehyde tripeptides or tetrapeptides, which selectively inhibit the proteasome, preserved substantial PKC-alpha and -epsilon protein from down-regulation by Bryo or phorbol-12-myristate-13-acetate. Lacta preserved active kinase in vivo, as shown by the retention of Bryo-induced autophosphorylated PKC-alpha. Concomitant with down-regulation, Bryo produced PKC-alpha and -epsilon species that were larger than the native proteins (80 and 90 kDa, respectively). Western blot analysis showed that the larger PKC-alpha species were ubiquitinylated. Treatment with Bryo plus Lacta synergistically increased multiubiquitinylated PKC-alpha, as expected if Bryo induces ubiquitinylation of PKC-alpha and Lacta blocks its degradation. Bryo also produced a 76-kDa, nonphosphorylated form of PKC-alpha and an 86-kDa form of PKC-epsilon. Phosphatase inhibitors decreased production of 76- and 86-kDa PKC-alpha and -epsilon by Bryo and preserved 80- and 90-kDa PKC-alpha and -epsilon, respectively. Our results suggest that the down-modulation of PKC-alpha and -epsilon occurs principally via the ubiquitin/ proteasome pathway. Dephosphorylation seems to predispose PKC to ubiquitinylation.
Soluble antigens from group B streptococci induce cytokine production in human blood cultures (1997)

Group B streptococcal antigens stimulated tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1), and IL-6 production in human blood cultures in a concentration- and time-dependent fashion. The minimal concentrations of type-specific polysaccharides, lipoteichoic acid, and group-specific polysaccharide required to produce these effects were, respectively, 0.01, 1, and 10 microg/ml. Cell separation experiments indicated that monocytes were the cell type mainly responsible for cytokine production. Time course studies indicated that TNF-alpha was released before the other cytokines. TNF-alpha, however, did not appear to directly induce IL-1beta, as shown by blockade experiments with anti-TNF-alpha antibodies. IL-6 levels were moderately but significantly decreased by anti-TNF-alpha. These data indicate that several products from group B streptococci are able to directly stimulate human monocytes to release TNF-alpha, IL-1beta, and IL-6. These findings may be clinically relevant, since proinflammatory cytokines can mediate pathophysiologic changes during sepsis.
Lactational performance of first-parity transgenic gilts expressing bovine alpha-lactalbumin in their milk (2002)

The goal of this study was to determine whether the presence of the bovine alpha-lactalbumin transgene in first-lactation gilts enhances lactational performance and litter growth. Transgenic and sibling nontransgenic gilts were bred to nontransgenic boars. Litters were standardized to 10 piglets within 24 h of farrowing. Milk production was measured by the weigh-suckle-weigh method on d 3, 6, 9, and 12 of lactation. Bovine alpha-lactalbumin was present in the colostrum and milk of transgenic gilts throughout lactation. The expression of the transgene was associated with alterations in composition of mammary secretions, especially in early lactation. Lactose concentrations were greater (P < 0.05) in mammary secretions of transgenic gilts during the first 12 h postpartum compared with controls. In contrast, total solids concentration in mammary secretions from transgenic gilts were lower (P < 0.05) relative to controls during the first 6 h postpartum. Transgenic gilts produced more milk than controls on d 3, 6, and 9 of lactation (P < 0.01). By d 12, differences in milk production between transgenic and control sows were no longer different. Lactose intake by transgenic-reared litters was greater than lactose intake by control-reared litters on d 6 of lactation (P < 0.05). Total solids intake was significantly greater (P < 0.05) by transgenic-reared litters on d 3 and 6 compared to control-reared litters. The day x genotype interaction on litter weight gain after birth was highly significant (P = 0.011), with transgenic-reared litters gaining weight at a greater rate than control-reared piglets. Expression of the transgene was associated with increased milk production in lactating gilts and increased growth of transgenic-reared piglets. Increased lactose synthesis in response to the presence of the transgene may result in increased milk production in early lactation, leading to increased milk component intake by transgenic litters, and ultimately to increased growth of litters reared by first-parity transgenic gilts.
Coherent Detection with Arrays of Photon-Counting Detectors (2006)

This talk will present the first experimental demonstration of coherent detection using an array of photon-counting detectors. These measurements use an array of Geiger-mode avalanche photodiodes with integrated CMOS timing circuitry, and show the potential for shot-noise-limited detection in the single-return-photon regime with comparable local oscillator power. The high-bandwidth timing circuitry and potential to fabricate large arrays enable range-resolved Doppler and angularly-resolved Doppler laser radar measurements. Laboratory results agree well with computer simulations.
A ±0.3 ppm Oven-Controlled MEMS Oscillator Using Structural Resistance-Based Temperature Sensing (2018)

This paper presents a 77.7-MHz silicon microelectromechanical-systems oven-controlled oscillator (MEMS OCXO) that uses the structural resistance ( ) of the resonator as an embedded temperature sensor. The exhibits a large temperature coefficient of resistance and is used as a self-temperature sensor to accurately and locally monitor the temperature of the resonator. A high-Q capacitive cross-sectional Lamé-mode resonator fabricated using the nanogap high aspect-ratio combined poly- and single-crystal silicon process (HARPSS) is used as the frequency selective element. A silicon resistor micro-oven is implemented on the MEMS die adjacent to the resonator and the ensemble is wafer-level packaged in vacuum to yield a 2 mm mm MEMS die. The micro-oven resistor is automatically controlled by the analog loop to provide active temperature stabilization for the resonator. A resistance temperature detector (RTD) circuit, high-gain loop filter, and heater amplifier are implemented as the analog micro-oven control loop. To further boost the frequency stability, a digital feedforward calibration path which uses the digitized RTD output to fine tune the phase shift of the sustaining amplifier is added to the system. The silicon MEMS OCXO achieves ±0.3-ppm frequency stability from -25 °C to 85 °C. The microresonator is interfaced with a sustaining amplifier implemented in Taiwan Semiconductor Manufacturing Company 0.35-s CMOS process, consuming 16 mA from a 3.2-V supply.
Antiviral lectins from red and blue-green algae show potent in vitro and in vivo activity against Hepatitis C virus (2012)

Hepatitis C virus (HCV) infection is a significant public health problem with over 170,000,000 chronic carriers and infection rates increasing worldwide. Current treatment options for HCV infection are limited to PEG-ylated interferon alpha, the nucleoside ribavirin and the recently approved HCV protease inhibitors telaprevir and boceprevir. Although showing significantly improved efficacy over the previous therapies, treatment with protease inhibitors has been shown to result in the rapid emergence of drug-resistant virus. Here we report the characterization of two novel proteins, originally isolated from natural product extracts, which demonstrate low or sub-nanomolar in vitro activity against both genotype I and genotype II HCV. These proteins inhibit viral infectivity by binding to the HCV envelope glycoprotein E2 and blocking viral entry into human hepatocytes. In addition, we demonstrate that the most potent of these agents, the 25 kDa protein griffithsin, comprised of domain-swapped homodimer of 12.7-kDa subunits, is bioavailable after subcutaneous injection and shows significant in vivo efficacy in reducing HCV viral titers in a mouse model with engrafted human hepatocytes.
Fluoropolymer surface modification for enhanced evaporated metal adhesion (1994)

Abstract Adhesion of evaporated Cu to Teflon PFA (polytetrafluoroethylene-co-perfluoroalkoxy vinyl ether) was greatly enhanced by plasma pretreatment. The efficiency of the treatment decreased in the following order: N2 > O2 > (N2 + H2) > (O2 + H2) > H2. X-ray photoelectron spectroscopy (XPS) showed the loss of fluorine and the incorporation of oxygen and nitrogen at the polymer surface. Among the gases, H2 was found to be the most efficient for fluorine elimination, and (N2 + H2) for surface functionalization. Based on this investigation, it is proposed that Cu reacts with both oxygen and nitrogen to form, respectively, Cu-O and Cu-N bonds at the interface but no reaction occurs with carbon and fluorine. While greater enhancement in polymer surface wettability and stronger interfacial reactions can account for the higher performance of N2 over O2 in improving adhesion, these effects cannot explain the lower efficiency of H2. Several possibilities are discussed, including surface cleaning, oxygen incorporation and the formation of weak boundary layers. Keywords: Teflon PFAplasmasurface modificationCu evaporationadhesionXPS
The removal of multiplicative, systematic bias allows integration of breast cancer gene expression datasets – improving meta-analysis and prediction of prognosis (2008)

Background The number of gene expression studies in the public domain is rapidly increasing, representing a highly valuable resource. However, dataset-specific bias precludes meta-analysis at the raw transcript level, even when the RNA is from comparable sources and has been processed on the same microarray platform using similar protocols. Here, we demonstrate, using Affymetrix data, that much of this bias can be removed, allowing multiple datasets to be legitimately combined for meaningful meta-analyses. Results A series of validation datasets comparing breast cancer and normal breast cell lines (MCF7 and MCF10A) were generated to examine the variability between datasets generated using different amounts of starting RNA, alternative protocols, different generations of Affymetrix GeneChip or scanning hardware. We demonstrate that systematic, multiplicative biases are introduced at the RNA, hybridization and image-capture stages of a microarray experiment. Simple batch mean-centering was found to significantly reduce the level of inter-experimental variation, allowing raw transcript levels to be compared across datasets with confidence. By accounting for dataset-specific bias, we were able to assemble the largest gene expression dataset of primary breast tumours to-date (1107), from six previously published studies. Using this meta-dataset, we demonstrate that combining greater numbers of datasets or tumours leads to a greater overlap in differentially expressed genes and more accurate prognostic predictions. However, this is highly dependent upon the composition of the datasets and patient characteristics. Conclusion Multiplicative, systematic biases are introduced at many stages of microarray experiments. When these are reconciled, raw data can be directly integrated from different gene expression datasets leading to new biological findings with increased statistical power.
Histogenic Instability in Tissue Culture-proliferated Strawberry Plants (1987)

Abstract A phenotypic and sexual analysis of Fragaria vesca ‘Albo-Marginata’ determined that the leaf variegation was of chimeral origin. Stable periclinal chimeras were established in vitro from runner tips. Plants were transferred to proliferation media containing 0.5 μ m IBA, 0.3 μ m GA 3 , and BA at either 0, 1.3, 4.4, or 13.2 μ m . Whereas the histogens of field-grown runner plants remained stable, more than 90% of the plantlets propagated in vitro varied from the original explants. Most variants were albino or were green, but some were mericlinal chimeras. Histological evidence indicated that many shoots were adventitious, arising from basal callus tissue or petioles. Chemical names used: 1 H -indole-3-butanoic acid (IBA); gibberellic acid (GA 3 ); N -(phenylmethyl)-1 H -purin-6-amine (BA).
Low sidelobe electronically scanned antenna using identical transmit/receive modules (1988)

A microwave network is described for use with identical transmit/receive modules such that amplitude-tapered aperture distributions are obtained with no loss. High-efficiency low-sidelobe patterns are calculated for the line source case with sidelobes as low as -27.5 dB. An optimum minimum-loss design is described for arbitrarily low sidelobes. The effects of errors on sidelobes are shown to be the same as in any array. The extension of the technique to the planar array case is presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Medical Diagnostic Decision Support Systems--Past, Present, And Future: A Threaded Bibliography and Brief Commentary (1994)

Articles about medical diagnostic decision support (MDDS) systems often begin with a disclaimer such as, "despite many years of research and millions of dollars of expenditures on medical diagnosticsystems, none is in widespread use at the present time." While this statement remains true in the sense that no single diagnostic system is in widespread use, it is misleading with regard to the state of the art of these systems. Diagnostic systems, many simple and some complex, are now ubiquitous, and research on MDDS systems is growing. The nature of MDDS systems has diversified over time. The prospects for adoption of large-scale diagnostic systems are better now than ever before, due to enthusiasm for implementation of the electronic medical record in academic, commercial, and primary care settings. Diagnostic decision support systems have become an established component of medical technology. This paper provides a review and a threaded bibliography for some of the important work on MDDS systems over the years from 1954 to 1993.
Energy Harvesting for Structural Health Monitoring Sensor Networks (2007)

This report has been developed based on information exchanges at a 2.5-day workshop on energy harvesting for embedded structural health monitoring (SHM) sensing systems that was held June 28-30, 2005, at Los Alamos National Laboratory. The workshop was hosted by the LANL/UCSD Engineering Institute (EI). This Institute is an education- and research-focused collaboration between Los Alamos National Laboratory (LANL) and the University of California, San Diego (UCSD), Jacobs School of Engineering. A Statistical Pattern Recognition paradigm for SHM is first presented and the concept of energy harvesting for embedded sensing systems is addressed with respect to the data acquisition portion of this paradigm. Next, various existing and emerging sensing modalities used for SHM and their respective power requirements are summarized, followed by a discussion of SHM sensor network paradigms, power requirements for these networks and power optimization strategies. Various approaches to energy harvesting and energy storage are discussed and limitations associated with the current technology are addressed. This discussion also addresses current energy harvesting applications and system integration issues. The report concludes by defining some future research directions and possible technology demonstrations that are aimed at transitioning the concept of energy harvesting for embedded SHM sensing systems from laboratory research to field-deployed engineering prototypes.
Sub-Marine Solution of Limestone in Relation to the Murray-Agassiz Theory of Coral Atolls (1916)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
Mutual Information as a Stereo Correspondence Measure (2000)

Traditional stereo systems often falter over changes in lighting between their two views. Unfortunately, such changes often occur when using stereo with a wide baseline or between images from different spectra. In this paper, we propose a new dense stereo correspondence similarity metric, mutual information, which has the potential to overcome such adverse conditions. We explore the strengths and weaknesses of this metric, both quantitatively and qualitatively, under a variety of conditions. Throughout the exploration, we compare mutual information to a more traditional cross-correlation stereo system. We show that mutual information performs under conditions in which traditional dense stereo fails.
<i>In</i>‐<i>situ</i> estimation of MOCVD growth rate via a modified Kalman filter (1996)

Abstract In‐situ laser reflectance monitoring of metal‐organic chemical vapor deposition (MOCVD) is an effective way to monitor growth rate and expitaxial layer thickness of a variety of III–V and II–VI semiconductors. Materials with low optical extinction coefficients, such as ZnTe/GaAs and AlAs/GaAs for a 6328 Å HeNe laser, are ideal for such an application. An Extended Kalman filter modified to include a variable forgetting factor was applied to the MOCVD systems. The filter was able to accurately estimate thickness and growth rate while filtering out process noise and cope with sudden changes in growth rate, reflectance drift, and bias. Due to the forgetting factor, the Kalman filter was successful, even when based on very simple process models.
Dimensional metrology using the optical comb of a mode-locked laser (2015)

In the field of dimensional metrology, significant technical challenges have been encountered with regard to large-scale object assembly, satellite positioning, control of the long-distance precision stage, and inspections of large steps or deep holes on semiconductor devices and multi-layered display panels. The key elements required are high speeds, a long dynamic measurable range, and good precision of measurements, and conventional methods can scarcely meet such requirements simultaneously. Promisingly, the advent of the optical comb has opened up numerous possibilities to break through practical limits by exploiting several of its unique features. These include inter-mode interference, a wide spectral bandwidth with a long coherence length and well-defined longitudinal modes. In this review, various dimensional metrological methods using the optical comb are introduced, describing their basic principles and applications in scientific as well as industrial areas.
Improved Electronic Properties from Third-Order SCC-DFTB with Cost Efficient Post-SCF Extensions (2012)

The present work outlines the implementation and performance of two cost efficient post-SCF extensions into the third-order SCC-DFTB code. The first one, the charge model 3 (CM3), corrects for errors in bond dipoles for an improved description of molecular charge distribution as compared to the standard Mulliken partitioning scheme. The second one focuses on the response of the charge density, that is, the electronic molecular polarizability, described inaccurately from SCC-DFTB due to the usage of a minimal atomic orbital basis. Here, a variational approach, based on scaled dipole integrals, was implemented, which clearly outperforms standard finite electric field approaches for polarizability calculations by approximately 1 order of magnitude. Both extensions in the present work rely on a set of empirical parameters, which were fitted against 112 organic molecules to match a reference data set from full density functional calculations with a large basis. As an achievement, notably improved electronic properties, that is, molecular dipole moments and polarizabilities, result from SCC-DFTB calculations at negligible additional computational cost. Furthermore, the accuracy of infrared and Raman intensities was tested as first-order derivatives of the new dipoles and polarizabilities as a function of normal mode vibrations. As a result, the current implementations cannot contribute to an improved prediction of relative intensity pattern from SCC-DFTB as compared to ab initio reference data.
Application of satellite remote sensing techniques in land use and land cover pattern analysis of the Nakuru-Nyahururu area (2002)

SPOT and LANDSAT-5 TM space images have been utilised to analyse land use cover characteristics and patterns in a section of the Aberdare Detachment system sub-basin, the Nakuru-Nyahururu area, Kenya. Good ground resolution of both data show significant details of the land use cover patterns and linear patterns of fault scarps. The synoptic view suggests, NNE, NE and NW trending scarps and associated grabens and plateaus. Spectral analysis of photographic products is made. A multitemporal approach is adopted and based on image characteristics, four areas of land use cover types have been identified: (i) agricultural and forest covered areas (ii) semi-arid intensely fault dissected north west (iii) down-faulted, tilted fault blocks (plateaus) (iv) Menengai volcanic caldera. A ground truth survey is made and with collateral data maps of land use are compiled.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Mechanistic insight into formation and changes of nanoparticles in MgF2 sols evidenced by liquid and solid state NMR (2012)

The fluorolytic sol-gel reaction of magnesium methoxide with HF in methanol was studied by (19)F, (1)H and (13)C liquid and solid state NMR. In (19)F NMR five different species were identified, three of which belong to magnesium fluoride nanoparticles, i.e. NMR gave access to local structures of solid particles in suspensions. The long-term evolution of (19)F signals was followed and along with (19)F MAS NMR experiments of sols rotating at 13 kHz mechanistic insights into the ageing processes were obtained.
Varactor Tuned Bandpass Filters Using Microstrip-Line Ring Resonators (2005)

This paper describes the fundamental characteristics and experimental results of newly developed tunable bandpass filters using rnicrostrip-line ring resonators. The experimental filter has a steeper attenuation slope and less circuit instability than conventional filters, and it seems to be a suitable filter for MICs or MMICs.
Formal verification of pipeline control using controlled token nets and abstract interpretation (1998)

Article Free Access Share on Formal verification of pipeline control using controlled token nets and abstract interpretation Authors: Pei-Hsin Ho Strategic CAD Labs, Intel Corporation Strategic CAD Labs, Intel CorporationView Profile , Adrian J. Isles Department of EECS University of California, Berkeley Department of EECS University of California, BerkeleyView Profile , Timothy Kam Strategic CAD Labs, Intel Corporation Strategic CAD Labs, Intel CorporationView Profile Authors Info & Claims ICCAD '98: Proceedings of the 1998 IEEE/ACM international conference on Computer-aided designNovember 1998 Pages 529–536https://doi.org/10.1145/288548.289082Online:01 November 1998Publication History 25citation247DownloadsMetricsTotal Citations25Total Downloads247Last 12 Months6Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF