gebhart/patrepeval huggingface dataset:
DNA probes for the rapid identification of medically important<i>Candida</i>species using a multianalyte profiling system (2005)

Recently, a new flow cytometric technology to detect multiple DNA target sequences in a single microtiter well plate was developed [multianalyte profiling (MAP) System, Luminex Corp., Austin, TX]. DNA probes, directed to the internal transcribed spacer 2 region of ribosomal DNA, were therefore designed to detect and differentiate PCR amplicons from six medically important Candida species using this system. Each probe was covalently linked to one of 100 available microsphere (bead) sets. Biotinylated PCR amplicons were then hybridized to the complementary probe on each bead set. Bound amplicons were detected fluorometrically using a streptavidin-linked reporter dye, R-phycoerythrin. Specific hybridization was noted for all six Candida species probes (mean sample-to-background ratio+/-standard error: Candida albicans, 58.7+/-1.2; Candida tropicalis, 53.2+/-3.8; Candida glabrata, 46.9+/-2.1; Candida parapsilosis, 59.9+/-1.6; Candida krusei, 54.7+/-3.7 vs. 0.9+/-0.03 for all heterologous Candida species DNA targets and vs. 1.0+/-0.1 for samples containing water instead of DNA; P < 0.001). The limit of test sensitivity was 0.5 pg of DNA. A sample could be processed and analyzed within 1 h post-PCR amplification. Therefore, the multianalyte profiling system was rapid, sensitive and specific for the detection and differentiation of the most medically important species of Candida.
PES reduction in magnetic disk drives via a low-TMR HGA and servo loop shaping (2005)

This paper presents a servo-mechanically combined strategy to reduce the position error signal (PES) in high-track density magnetic disk drives. In this strategy, a special head-gimbal assembly (HGA) is designed so that it can move not only vertically to the disk but also across the data tracks. It is shown that such HGAs can reduce the track misregistration (TMR) due to disk vibration, by allowing the head to move in such a way that will compensate the off-track caused by disk bending. A further reduction in the final PES is then attempted by redesigning the servo loop shape taking the reduced disk-mode disturbance into consideration. The well-known random neighborhood search method is applied to find a set of servo gains that best approximates a desired loop shape determined based on empirical knowledge. Finally, the efficacy of the proposed strategy is demonstrated experimentally by using actual sample drives.
A new storage scheme for streaming media server cluster (2011)

We report on the design, implementation of a non equal scheme in streaming media data storage. According to the characteristics of the streaming media and considering the human beings usage, our scheme combines n-way mirroring and erasure-coding together to storage streaming media data. It is make good tradeoffs between system fault tolerance and sufficient quality of service streams. The system has the optimal storage efficiency and the remarkably fault-tolerant capability. At last the design of the non-equal streaming media data cluster storage system is introduced in detail, and an intensive performance evaluation study on it is performed.
The need for improving the exploration operators for constrained optimization problems (2002)

Several specific methods have been proposed for handling nonlinear constraints. These methods have to bring individuals in the feasible space, and help to explore and exploit efficiently the feasible domain. However, even if this domain is not sparse, this paper demonstrates that the exploration capacity of standard reproduction operators is not optimal when solving constrained problems. The logarithmic mutation operator presented in this paper has been conceived to explore both locally and globally the search space. As expected, it exhibits a robust and efficient behavior on a constrained version of the Sphere problem, compared to some other standard operators. Associated with BLX-0.5 crossover and a special ranking selection taking the constraints into account, the logarithmic mutation allows a GA to often reach better performance than several well known methods on a set of classical test cases.
Microcavity Polariton Splitting of Intersubband Transitions (2003)

The optical response of the intersubband excitation of multiple two-dimensional electron gases within a semiconductor microcavity has been studied through angle-dependent reflectance measurements. Using a resonator based on total internal reflection, a clear splitting of about 14 meV of the coupled intersubband cavity modes is observed from 10 K to room temperature, with resulting polaritonlike dispersion. The experimental findings are in good agreement with theoretical calculations performed in a transfer-matrix formalism.
Emission Spectra and Lifetimes of R6G Dye on Silica-Coated Titania Powder (2002)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTEmission Spectra and Lifetimes of R6G Dye on Silica-Coated Titania PowderLydia Bechger, A. Femius Koenderink, and Willem L. VosView Author Information Van der Waals-Zeeman Instituut, Universiteit van Amsterdam, Valckenierstraat 65, NL-1018 XE Amsterdam, The Netherlands Cite this: Langmuir 2002, 18, 6, 2444–2447Publication Date (Web):February 20, 2002Publication History Received27 September 2001Revised18 December 2001Published online20 February 2002Published inissue 1 March 2002https://pubs.acs.org/doi/10.1021/la011485nhttps://doi.org/10.1021/la011485nbrief-reportACS PublicationsCopyright © 2002 American Chemical SocietyRequest reuse permissionsArticle Views626Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Dyes and pigments,Layers,Oxides,Photonics,Silica Get e-Alerts
Evidence That Apolipoprotein A-I <sub>Milano</sub> Has Reduced Capacity, Compared With Wild-Type Apolipoprotein A-I, to Recruit Membrane Cholesterol (1997)

Human carriers of apolipoprotein (apo) A-IMilano are heterozygous for an Arg173-->Cys substitution in the apoA-I primary sequence; despite severe reductions in HDL cholesterol concentrations, affected individuals do not develop coronary heart disease, suggesting that apoA-IMilano may possess antiatherogenic properties. As the beneficial effects of wild-type apoA-I are linked to its role in HDL cholesterol transport, we examined the capacity of apoA-IMilano to recruit cell cholesterol and activate lecithin:cholesterol acyltransferase (LCAT) (two key events in the antiatherogenic reverse cholesterol transport pathway). ApoA-IMilano and wild-type apoA-I were expressed in Chinese hamster ovary cells, and their ability to recruit membrane phospholipid and cholesterol for the assembly of nascent HDL was compared. Both clonal cell lines exhibited similar levels of apolipoprotein accumulation in serum-free medium (approximately 2 micrograms/mg cell protein per 24 hours), and 15% of each apolipoprotein was associated with membrane lipids to form nascent HDL (d = 1.063 to 1.21 g/mL). SDS-PAGE showed that a majority (66 +/- 12%) of the lipidated apoA-IMilano was in the homodimer form. Compositional analyses revealed that apoA-IMilano nascent HDL had a significantly lower (P < .001) unesterified cholesterol/phospholipid mole ratio (0.47 +/- 0.10) than wild-type apoA-I complexes (1.29 +/- 0.14), indicating that apoA-IMilano had a reduced capacity to recruit cell cholesterol. In addition to the reduced unesterified cholesterol/phospholipid ratio, apoA-IMilano nascent HDL consisted mostly of small 7.4-nm particles compared with wild-type apoA-I, in which 11- and 9-nm particles predominated. Despite these changes in nascent HDL particle size and composition, apoA-IMilano activated LCAT normally. We conclude that, even though apoA-IMilano is a normal activator of LCAT, it is less efficient that wild-type apoA-I in recruiting cell cholesterol, suggesting that the putative antiatherogenic properties attributed to apoA-IMilano may be unrelated to the initial stages of reverse cholesterol transport.
An Empirical Investigation of the Critical Determinants for the Adoption of E-Market in Australian Small-and-Medium Sized Enterprises (2010)

This review of the literature presents the data on the history of development of the methods of staining of histological sections using the natural dye, hematoxylin. The content of solutions of the most commonly used alum hematoxylins is described. The information is given on the modern staining methods, in which alum and iron hematoxylins are used, as well as on the hematoxylins, in which vanadium, lead, bismuth, uranium and other metals' salts are applied as mordants.
Simultaneous transmit and receive polarimetric synthetic aperture radar based on digital beamforming (2015)

Synthetic aperture radar is a vital tool for imaging and remote sensing regardless of the villainous weather. Polarimetric synthetic aperture radar obtains the polarization characteristic of targets through polarized wave. This paper presents a novel technique, the combination of digital beamforming and the polarization code-diversion mode, to suppress the range ambiguity of the subapertures based on the simultaneous transmit and reception PolSAR. In the simulation the nulling steering technique proves to be better for the ambiguity mitigation than the conventional radar.
Cutaneous Metastasis: A Meta-Analysis of Data (2003)

This meta-analysis greatly increases the total number of cases available for the analysis of cutaneous metastases and provides a better overall view of this topic than was previously possible.
Broadband Cavity Ring‐Down Spectroscopy (2009)

This chapter contains sections titled: Introduction The Time and Wavelength Evolution of a Single Ring-Down Event Two-Dimensional Techniques: Resolving Broadband Cavity Output in Time and Wavelength One-Dimensional Techniques: Time or Wavelength How to Extract Quantitative Information from Broadband Spectra Optimising the Sensitivity of a Broadband Measurement Applications of Broadband Cavity Methods References
Effects of absorbed components of Saiboku-to on the release ofleukotrienes from polymorphonuclear leukocytes of patients withbronchial asthma (2001)

Previous studies confirmed the efficacy of the Chinese herbal remedy Saiboku-to in patients with steroid-dependent asthma. We studied 8 phenolic compounds, isolated from the urine of patients receiving Saiboku-to, with respect to their effects on leukotriene (LT) release by polymorphonuclear leukocytes (PMN) obtained from 6 patients with atopic asthma and 8 healthy subjects. The compounds (0.01-10 micrograms/ml) were incubated with Ca2+ ionophore (A23187)-stimulated PMN, and concentrations of leukotriene B4 (LTB4) and leukotriene C4 (LTC4) in the supernatant were measured by EIA. Each compound suppressed the release of LTB4 and LTC4 by PMN obtained from healthy and asthmatic subjects. In particular, baicalein and magnolol were 5-10 times more potent than azelastine, an antiallergic drug, and significantly suppressed LTC4 release by PMN obtained from asthmatic patients, as compared with healthy subjects. Suppression of LTC4 release by these compounds may play an important role in the clinical efficacy of Saiboku-to.
A survey design for monitoring butterflies (2013)

A technique commonly used for surveying butterflies is the Pollard - Yates method (Pollard, 1977; Pollard and Yates, 1993). This method was originally developed for surveying butterflies at Monks Wood, UK (Pollard et al., 1975) and is now widely used in the UK. The method is based on a count of the number of butterflies seen along a fixed survey route. The Pollard - Yates method is easy to implement in the field but it does not allow for differences in the ability to detect butterflies among sites. Line transect sampling (Buckland et al., 1993) has similar survey methods to Pollard - Yates except that distance data is collected and used to estimate a detection function. Therefore, one of the main advantages of line transect sampling is that it provides estimates of abundance that are comparable among sites regardless of the sites' "butterfly-detectability". In addition, it can be used to derive unbiased estimates of local-site densities provided key assumptions are met (Brown and Boyce, 1996, 1998). We introduce some aspects of survey design for a monitoring scheme established for Karner blue butterflies (Lycaeides Melissa samuelis) in Wisconsin, USA.
Corneal Edema and Intraocular Pressure (1965)

The relationship between intraocular pressure, corneal hydration, and transparency has received attention in the past and the subject deserves a review. As early as 1873 Leber1had found that in freshly enucleated eyes an intraocular pressure of around 200 mm Hg caused bullous epithelial edema within a few hours. He also described the oft-quoted experiment of inducing stromal opacification by squeezing the eye, and the clearing which takes place when pressure is released. Bullot (1904)2suspended rabbit eyes, with epithelium removed, in sodium chloride solutions of varying tonicity. The use of hypotonic solutions caused a temporarily increased intraocular pressure and the corneas remained relatively thin and transparent. Conversely, when the eyes were bathed in hypertonic saline solutions, low intraocular pressure and swollen corneas were the result. The author ascribed the different hydrations of the cornea to the level of the intraocular pressure. Kinsey and Cogan (1942)3immersed
Rules based adaptation of Smith predictor (2003)

The investigation deals with the employment of a Smith predictor as an improved working mechanism for overcoming the negative effects arising in control systems with a long time delay. As a model-based algorithm it imposes a stringent restriction over systems behavior by parametric uncertainty. The adaptation algorithm proposed in this paper uses data, derived by analysis of control system performance. It determines a quantitative correction for uncertainty compensation of model parameters and the allowable rate of increasing the gain coefficient of the conventional PI-controller. Special attention is paid and a significant experimental volume of the investigation is carried out for estimation and excluding the effects of the available noise in the system with a specific intensity and spectral density on method accuracy.
New designs of micromachined vibrating rate gyroscopes with decoupled oscillation modes (2002)

This paper reports on new vibrating comb-drive rate gyroscope designs featuring decoupling of the actuation and the detection mode. Analytical calculations as well as FEM and system simulations are carried and the main drawbacks of these sensors are investigated which are especially the frequency mismatch of the two oscillation modes, the insufficient bandwidth and the effects of levitation. Concepts and simulations of electrostatically controlling both the resonance frequency and the bandwidth are presented. Within these concepts no mechanical balancing of the devices is required and thus calibration costs are reduced. Simulations of a new quasi-rotating rate gyroscope show, that a resolution of 0.1/spl deg//s is possible at a bandwidth of 50 Hz with 6 mm/sup 3/ sensor area only and that the effects of levitation are actually compensated.
Application of Particle Swarm Optimization Algorithm in Bellow Optimum Design (2007)

In this paper, Particle Swarm Optimization Algorithm (PSOA) is used in problem of the bellow optimum design constraints, in which the design variables are discrete, implement bellow optimum design by PSOA, an augmented objective function is constructed based on penalty function and new updating scheme of penalty parameter s is proposed. A Discrete PSOA (DPSOA) is proposed. The mathematic model bellow optimum design is established. Through numerical examples of bellow design, comparing the results of examples by proposed DPSOA with the theory solutions by Net method, shows that the particle swarm optimization algorithm can applied to the bellow optimum design successfully satisfactory results by DPSOA are obtained, which is discrete optimal solution in the feasible domain.
INALSYS: a layout automation system based on analog layout constraints (2005)

An analog layout system that can handle analog constraints is presented in this paper. In order to process the analog layout constraints, parameterizable module library and the effective floorplanning model that encapsulates well-merging is proposed. Also, global routing algorithm is modified by adopting divide-by-triangle method. This layout system has been tested on several benchmark circuits and displayed comparable results to the conventional layout system in terms of layout quality, expandability and execution time.
Silica-on-silicon optical sensor based on integrated waveguides and microchannels (2005)

The operation of a novel optical device sensitive to the refractive index of a small (<1/spl times/10/sup -12/ m/sup 3/) volume of liquid is demonstrated experimentally. The structure is a silica-on-silicon three-core directional coupler with two quasi-circular microchannels, approximately three micrometers in diameter, located between the cores. The operation of the device is demonstrated by introducing liquid into the microchannels and varying the refractive index of the liquid through a temperature change.
Sticky Caveats in an Otherwise Glowing Report: Oligomerizing Fluorescent Proteins and Their Use in Cell Biology (2002)

Fluorescent proteins from sea creatures have revolutionized the study of cell biology and signal transduction in many ways. Zacharias discusses some of the technical caveats to working with these proteins when they are fused to cellular proteins to track protein localization and interactions. Special attention is paid to problems arising from oligomerization of these fluorescent proteins and how that impacts protein interactions detected by fluorescence resonance energy transfer (FRET).
cAMP and cGMP in nasal mucus related to severity of smell loss in patients with smell dysfunction (2008)

These results confirm the association of biochemical changes in cyclic nucleotides with systematic losses of smell acuity. These results confirm the usefulness of the psychophysical methods we defined to determine the systematic classification of smell loss severity. These changes can form the basis for the biochemical definition of smell loss among some patients with smell loss as well as for their therapy.
Short-time fractional Fourier methods for the time-frequency representation of chirp signals (2003)

The fractional Fourier transform (FrFT) provides a valuable tool for the analysis of linear chirp signals. This paper develops two short-time FrFT variants which are suited to the analysis of multicomponent and nonlinear chirp signals. Outputs have similar properties to the short-time Fourier transform (STFT) but show improved time-frequency resolution. The FrFT is a parameterized transform with parameter, a, related to chirp rate. The two short-time implementations differ in how the value of a is chosen. In the first, a global optimization procedure selects one value of a with reference to the entire signal. In the second, a values are selected independently for each windowed section. Comparative variance measures based on the Gaussian function are given and are shown to be consistent with the uncertainty principle in fractional domains. For appropriately chosen FrFT orders, the derived fractional domain uncertainty relationship is minimized for Gaussian windowed linear chirp signals. The two short-time FrFT algorithms have complementary strengths demonstrated by time-frequency representations for a multicomponent bat chirp, a highly nonlinear quadratic chirp, and an output pulse from a finite-difference sonar model with dispersive change. These representations illustrate the improvements obtained in using FrFT based algorithms compared to the STFT.
Experimental local therapy of human melanoma with lytic magainin peptides (1995)

Magainin peptides and model amphipathic peptides exhibit antibiotic activity and are also cytolytic for transformed human cells. Here we demonstrate in vitro that MSI-511 (an all-D amino-acid model magainin peptide) and MSI-130 (a margainin analogue) were more lytic for 17 human melanomas than for normal melanocytes. Melanomas established s.c. in athymic nude mice and then injected once with the peptide MSI-511 completely disappeared in 6 out of 9 animals, whereas a control peptide had no effect. Murine skin at the tumor injection site was initially affected, but healed within 2 weeks with minimal scarring. Similarly, accelerated healing was seen in human skin grafted to SCID mice and injected with MSI-511. Our results indicate that lytic magainin peptides can be used for local tumor therapy with minimal long-term damage to normal tissues.
Pharmacogenetics of lithium prophylaxis in mood disorders: Analysis of COMT, MAO‐A, and Gβ3 variants (2002)

We studied the possible association between the prophylactic efficacy of lithium in mood disorders and the following gene variants: catechol-O-methyltransferase (COMT) G158A, monoamine oxydase A (MAO-A) 30-bp repeat, G-protein beta 3-subunit (Gbeta3) C825T. A total of 201 subjects affected by bipolar (n = 160) and major depressive (n = 41) disorder were followed prospectively for an average of 59.8 months and were typed for their gene variants using PCR techniques. COMT, MAO-A, and Gbeta3 variants were not associated with lithium outcome, even when possible stratification effects such as sex, polarity, age at onset, duration of lithium treatment, and previous episodes were included in the model. The pathways influenced by those variants are not therefore involved with long-term lithium outcome in our sample.