gebhart/patrepeval huggingface dataset:
Energetics of arginine-4 substitution mutants in the N-terminal cooperativity domain of T4 gene 32 protein (1993)

Gene 32 protein (gp32) from bacteriophage T4 is a sequence-nonspecific single-strand (ss) nucleic acid binding protein which binds highly cooperatively to ss nucleic acids. The N-terminal "B" or basic domain (residues 1-21) is known to be required for highly cooperative binding by gp32 (where K(app) = K(int) omega, omega > or = 500), since its removal results in a protein which binds ss nucleic acids noncooperatively (omega = 1). In this paper, we probe the molecular details of cooperative binding by gp32 by physicochemical characterization of a set of four single amino acid substitution mutants of Arg4: Lys4 (R4K gp32), Gln4 (R4Q gp32), Thr4 (R4T gp32), and Gly4 (R4G gp32). The qualitative ranking of binding affinities to poly(A) is wild-type > or = R4K > R4Q > R4T > R4G > gp32-B (gp32 lacking the first 21 amino acids). The occluded site size is n(app) = 7.5 +/- 0.5 for all gp32s. Resolution of K(int) and omega for wild-type, R4K, R4Q, and R4T gp32s was estimated under conditions of low lattice saturation (v < or = 0.011) using multiple reverse fluorescence titrations collected at 10 mM Tris-HCl, pH 8.1, 20 degrees C, and a NaCl concentration where K(app) was (2-4) x 10(6) M-1 for each gp32 on the ribohomopolymer poly(A). Binding parameters for all gp32s were obtained directly or compared by conservative extrapolation of the [NaCl] dependence of K(app) to 0.20 M NaCl, 20 degrees C, pH 8.1. The magnitude of omega was then assumed not to vary with [NaCl] (shown for R4T gp32), allowing estimation of K(int) at 0.20 M NaCl. We find that R4K gp32 binds to poly(A) with an overall affinity (K(app)) which is 2-3-fold lower than wild-type gp32, while omega for each molecule seems indistinguishable (wild-type gp32, omega approximately 800-1300; R4K gp32, omega approximately 600-1200). Surprisingly, R4Q gp32 is characterized by an omega also not readily distinguishable from the wild-type and R4K proteins (omega approximately 800-4400), while K(app) is reduced about 10-fold. This mutant also shows a significantly reduced [NaCl] dependence of the binding to poly(A). R4T gp32 binds about 10-fold weaker than the Q mutant. It exhibits an omega ranging from 300 to 700 and a substantially reduced [NaCl] dependence (delta log K(int)/delta log [NaCl] = -1.4 from 0.10 to 0.20 M NaCl), indicative of significant perturbations in both K(int) and omega terms.(ABSTRACT TRUNCATED AT 400 WORDS)
Simultaneous Lateral Interbody Fusion and Posterior Percutaneous Instrumentation: Early Experience and Technical Considerations (2015)

Lumbar fusion surgery involving lateral lumbar interbody graft insertion with posterior instrumentation is traditionally performed in two stages requiring repositioning. We describe a novel technique to complete the circumferential procedure simultaneously without patient repositioning. Twenty patients diagnosed with worsening back pain with/without radiculopathy who failed exhaustive conservative management were retrospectively reviewed. Ten patients with both procedures simultaneously from a single lateral approach and 10 control patients with lateral lumbar interbody fusion followed by repositioning and posterior percutaneous instrumentation were analyzed. Pars fractures, mobile grade 2 spondylolisthesis, and severe one-level degenerative disk disease were matched between the two groups. In the simultaneous group, avoiding repositioning leads to lower mean operative times: 130 minutes (versus control 190 minutes; p = 0.009) and lower intraoperative blood loss: 108 mL (versus 93 mL; NS). Nonrepositioned patients were hospitalized for an average of 4.1 days (versus 3.8 days; NS). There was one complication in the control group requiring screw revision. Lateral interbody fusion and percutaneous posterior instrumentation are both readily accomplished in a single lateral decubitus position. In select patients with adequately sized pedicles, performing simultaneous procedures decreases operative time over sequential repositioning. Patient outcomes were excellent in the simultaneous group and comparable to procedures done sequentially.
Molecular Epidemiology of Outbreaks of Gastroenteritis Associated with Small Round-Structured Viruses in East Anglia, United Kingdom, During the 1996–1997 Season (1999)

During the winter season from November 1996 to May 1997, 550 fecal specimens were submitted from 94 outbreaks of gastroenteritis occurring in East Anglia, United Kingdom. These specimens were tested for the presence of small round-structured viruses (SRSVs) by electron microscopy, reverse transcriptase PCR, or both methods. SRSVs were shown to be associated with 64 of 94 (68%) of these outbreaks, of which 16 (25%) outbreaks occurred at a single location (Southend) within the region. Twenty-four specimens from 13 of the 16 SRSV-positive outbreaks occurring in Southend were available for genomic analysis, in which divergence within the RNA polymerase region of the SRSV genome was investigated. A further 27 specimens from 17 other SRSV-associated outbreaks, occurring at different locations within East Anglia but at the same time as those at Southend, were also studied. Fifty of the total of 51 (98%) specimens studied were shown to belong to genogroup II, and within this genogroup, 49 of 50 (98%) specimens were shown to be Grimsby-like viruses, with only one Mexico-like strain. Furthermore, phylogenetic analysis of the Grimsby-like viruses indicated clusterings according to the geographical location of the outbreak. One specimen contained a virus belonging to genogroup I, and this had the greatest sequence identity (83%) with Southampton virus.
Additive Manufacturing-Oriented Design of Graded Lattice Structures Through Explicit Topology Optimization (2017)

In the present work, a new approach for designing graded lattice structures is developed under the moving morphable components/voids (MMC/MMV) topology optimization framework. The essential idea is to make a coordinate perturbation to the topology description functions (TDF) that are employed for the description of component/void geometries in the design domain. Then, the optimal graded structure design can be obtained by optimizing the coefficients in the perturbed basis functions. Our numerical examples show that the proposed approach enables a concurrent optimization of both the primitive cell and the graded material distribution in a straightforward and computationally effective way. Moreover, the proposed approach also shows its potential in finding the optimal configuration of complex graded lattice structures with a very small number of design variables employed under various loading conditions and coordinate systems.
The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes. (1994)

Throughout the purification of the mdm-2 or mdm-2-p53 protein complexes, a protein with a molecular weight of 34,000 was observed to copurify with these proteins. Several monoclonal antibodies directed against distinct epitopes in the mdm-2 or p53 protein coimmunoprecipitated this 34,000-molecular-weight protein, which did not react to p53 or mdm-2 polyclonal antisera in a Western immunoblot. The N-terminal amino acid sequence of this 34,000-molecular-weight protein demonstrated that the first 40 amino acids were identical to the ribosomal L5 protein, found in the large rRNA subunit and bound to 5S RNA. Partial peptide maps of the authentic L5 protein and the 34,000-molecular-weight protein were identical. mdm-2-L5 and mdm-2-L5-p53 complexes were shown to bind 5S RNA specifically, presumably through the known specificity of L5 protein for 5S RNA. In 5S RNA-L5-mdm-2-p53 ribonucleoprotein complexes, it was also possible to detect the 5.8S RNA which has been suggested to be covalently linked to a percentage of the p53 protein in a cell. These experiments have identified a unique ribonucleoprotein complex composed of 5S RNA, L5 protein, mdm-2 proteins, p53 protein, and possibly the 5.8S RNA. While the function of such a ribonucleoprotein complex is not yet clear, the identity of its component parts suggests a role for these proteins and RNA species in ribosomal biogenesis, ribosomal transport from the nucleus to the cytoplasm, or translational regulation in the cell.
Polypyrrole and Composite Electrodes for Electrochemical Supercapacitors (2013)

Abstract not Available.
Analysis and design of enhanced planar devices using multiconductor transmission lines with interconnected alternate lines (2014)

Novel capabilities and applications of multiconductor transmission lines with interconnected alternate lines have been analyzed in this work. The interconnections among alternate strips broaden the operating frequency band by eliminating undesired resonances and allow the use of simplified analytical models. Phase shifters, baluns, reconfigurable systems for the characterization of balanced circuits, ultra-wideband differential bandpass filters and quasi-elliptic bandpass filters have been proposed and demonstrated. For all these circuits a systematic design procedure have been derived and validated by means of experimental work. The excellent agreement between the measurements and the predicted results validates the proposed procedures as reliable and quick design techniques.
Highly‐accelerated volumetric brain examination using optimized wave‐CAIPI encoding (2019)

2 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2019;50:961-974.
Implementation of a class of asymmetrical neural networks with application to an a‐d converter (1992)

Abstract This paper attempts to clarify the problems which occur in implementation of a large‐scale neural network. First, a neural A‐D converter with CMOS circuits is designed. Asymmetrical synaptic weights were used to avoid the local minima problem for a neural A‐D converter. Furthermore, a chip is fabricated using 4 μm CMOS technology. Its successful action as an A‐D converter is verified and some possible causes of misoperation of the present circuit are presented.
Human Papilloma Virus Infection and Overexpression of P53 Protein in Bilharzial Bladder Cancer (2001)

HPV may thus be implicated in the etiology of bilharzial bladder cancer, but a definite causal relationship remains to be demonstrated. HPV together with p53 alterations work in synergy to accelerate the carcinogenic process, as there was concordance in the results of both parameters in 24/41 (58.5%) cases.
Gas Diffusion Impedance in Characterization of Solid Oxide Fuel Cell Anodes (1999)

Impedance spectra obtained on high‐performance Ni/yttria stabilized zirconia (YSZ) cermet anodes at 1000°C in gas mixtures are considered. An impedance arc with a characteristic frequency of about 10 to 100 Hz is related to finite diffusion limitation by considering the dependence on water partial pressure, apparent thermal activation, and effect of the inert gas component. By experiments in different test geometries the diffusion zone is demonstrated to be a stagnant gas layer outside the anode structure. An estimated stagnant gas layer thickness of about 1 mm is arrived at. The diffusion impedance is demonstrated to be avoidable by using a setup geometry with two coupled electrodes in direct electrical contact through opposing sides of a contacting mesh. © 1999 The Electrochemical Society. All rights reserved.
Effects of Short and Long Duration Hypothyroidism and Hyperthyroidism on the Plasma Adrenocorticotropin and Corticosterone Responses to Ovine Corticotropin-Releasing Hormone in Rats* (1991)

We report here a study of the plasma ACTH and corticosterone responses to synthetic ovine CRH (oCRH) in hypothyroid and hyperthyroid rats studied 7, 15, and 60 days after either thyroidectomy or the administration of pharmacological doses of T4. The purpose of this study was to further clarify the time-dependent effects of alterations in thyroid status on the functional integrity of the hypothalamic-pituitary-adrenal axis and to aid in the interpretation of the oCRH stimulation test in hypo- and hyperthyroid states. Our data demonstrate that hypothyroid rats have a significant reduction in the cerebrospinal fluid (CSF) levels of corticosterone and a significant decrease in adrenal weight in association with significant increases in the plasma ACTH response to oCRH. On the other hand, the corticosterone response to the ACTH released during the oCRH stimulation test was significantly reduced in hypothyroidism. With increasing duration of thyroidectomy-induced hypothyroidism, there was a progressive fall in CSF corticosterone levels, a progressive increase in the plasma ACTH response to oCRH, and a gradual normalization of the corticosterone responses to the ACTH released during oCRH stimulation. Our findings in hyperthyroid rats were generally the converse of those seen in hypothyroidism. Hence, there was a significant increase in the CSF levels of corticosterone and a significant increase in adrenal weight in association with an initial slight decrease in the ACTH response to oCRH. On the other hand, the corticosterone response to the ACTH released during oCRH stimulation was significantly increased. There was a gradual increase in the magnitude of the rise in CSF corticosterone levels with time, as well as a gradual normalization of adrenocortical responses during oCRH stimulation. The ACTH plasma clearance rates were similar in hypo-, hyper-, and euthyroid rats. Our data do not permit definitive identification of the precise locus in the hypothalamic-pituitary-adrenal axis that is principally affected by experimentally induced alterations in thyroid status. However, these data are most compatible with a subtle hypothyroid-induced centrally mediated adrenal insufficiency and a subtle hyperthyroid-induced centrally mediated hypercortisolism. These data also suggest that alterations in hypothalamic-pituitary-adrenal function in states of disturbed thyroid function become somewhat more pronounced as the duration of thyroid dysfunction increases. The fact that pituitary-adrenal responses to oCRH are consistently altered in states of thyroid dysfunction may be relevant to the clinical interpretation of oCRH stimulation tests.(ABSTRACT TRUNCATED AT 400 WORDS)
Cellular microRNA detection with miRacles: microRNA- activated conditional looping of engineered switches (2019)

MicroRNAs are short noncoding regulatory RNAs that are increasingly used as disease biomarkers. Detection of microRNAs can be arduous and expensive and often requires amplification, labeling, or radioactive probes. Here, we report a single-step, nonenzymatic microRNA detection assay using conformationally responsive DNA nanoswitches. Termed miRacles (microRNA-activated conditional looping of engineered switches), our assay has subattomole sensitivity and single-nucleotide specificity using an agarose gel electrophoresis readout. We detect cellular microRNAs from nanogram-scale RNA extracts of differentiating muscle cells and multiplex our detection for several microRNAs from one biological sample. We demonstrate 1-hour detection without expensive equipment or reagents, making this assay a compelling alternative to quantitative polymerase chain reaction and Northern blotting.
Implementation of a fiber-optic delay-line memory (1992)

The construction and operation of a 50-MHz 64 x 16 bit fiber-optic bit-serial delay-line memory is described. It consists of LiNbO(3) directional coupler switches, fused-fiber couplers, and a 4.17-km fiber loop. It is a subsystem of a bit-serial optical computer under construction by our group. We discuss delay and clock source stability requirements for the long delay line in the face of a limited phase error tolerance. The reliability testing of the memory subsystem is described. The degradation of data in the memory loop as the phase error tolerance is exceeded by a small amount is studied through the temperature dependence of the memory loop. Data are presented for the memory-loop stability with respect to temperature variations. The memory subsystem design and construction is presented. The results of these experiments support the feasibility of a 100-MHz 128 x 16 bit memory.
Power control in a cellular system (2002)

Presents a power control algorithm based on carrier to interference ratio and simulation results showing a substantial capacity gain in a cellular environment. The power control algorithm is fully decentralized and uses only knowledge of transmitted power and received carrier to interference ratio in the current link.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
ChemInform Abstract: Antiinflammatory Activity of Novel Aryl‐ and Aroylthioureas. (2002)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Fluoride and Monofluorophosphate Analysis (1983)

The commonest method for fluoride analysis is the fluorideion-specific electrode. Other methods for fluoride analysis include volumetric, colorimetric, gas chromatographic, ion chromatography and nondestructive nuclear methods. The monofluorophosphate (MFP) ion is analyzed directly by ion exchange, ion chromatography or paper chromatography. The MFP ion is also determined by analysis before and after hydrolysis of the fluoride ion. Only a limited number of reports on <sup>18</sup>F- and <sup>32</sup>P-labeled MFP have been made.
Fluorescence Sensing of Anions via Intramolecular Excimer Formation in a Pyrophosphate-Induced Self-Assembly of a Pyrene-Functionalized Guanidinium Receptor (1999)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTFluorescence Sensing of Anions via Intramolecular Excimer Formation in a Pyrophosphate-Induced Self-Assembly of a Pyrene-Functionalized Guanidinium ReceptorSeiichi Nishizawa, Yuichi Kato, and Norio TeramaeView Author Information Department of Chemistry, Graduate School of Science Tohoku University, Aoba-ku, Sendai 980-8578, Japan Cite this: J. Am. Chem. Soc. 1999, 121, 40, 9463–9464Publication Date (Web):September 24, 1999Publication History Received6 May 1999Revised13 August 1999Published online24 September 1999Published inissue 1 October 1999https://pubs.acs.org/doi/10.1021/ja991497jhttps://doi.org/10.1021/ja991497jrapid-communicationACS PublicationsCopyright © 1999 American Chemical SocietyRequest reuse permissionsArticle Views3060Altmetric-Citations332LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anions,Fluorescence,Oligomers,Receptors,Self organization Get e-Alerts
Effect of high‐temperature degumming on the constituents and structure of cotton stalk bark fibers (2012)

Abstract The high‐temperature degumming technology of cotton stalk bark fiber was studied, and the effects of the caustic soda concentration on the constituents, structure, and thermal degradation of the cotton stalk bark fiber were examined. The morphology, structure, and thermal degradation of the cotton stalk bark fiber after high‐temperature degumming were investigated through scanning electron microscopy, Fourier transform infrared (FTIR) spectroscopy, wide‐angle X‐ray diffraction, and thermogravimetric analysis. The results indicate that the high‐temperature degumming process was effective for removing hemicelluloses and lignin and could improve the thermal stability of the cellulose. The raw and degummed cotton stalk bark fibers both showed the structure of cellulose I, according to the FTIR spectroscopy and X‐ray diffraction results. It was interesting to note that the crystallinity and crystallinity index were related to the caustic soda concentration. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Liquid-Crystalline Phases of Colloidal Dispersions of Layered Double Hydroxides (2003)

Colloidal dispersions of Mg/Al LDH platelets show liquid-crystal phase at a certain particle concentration. Threaded texture observed with polarized microscopy indicated it was typical of a nematic phase, which can often be found in traditional liquid crystals.
Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing (2002)

A multicomponent complex of proteins and RNA is assembled on the newly synthesized pre-mRNA to form the spliceosome. This complex catalyzes a two-step transesterification reaction required to remove the introns and ligate the exons. To date, only six proteins have been found necessary for the second step of splicing in yeast, and their human homologs have been identified. We demonstrate that the addition of the selective chelator of zinc, 1,10-phenanthroline, to an in vitro mRNA splicing reaction causes a dose-dependent inhibition of the second step of splicing. This inhibition is accompanied by the accumulation of spliceosomes paused before completion of step two of the splicing reaction. The inhibition effect on the second step is due neither to snRNA degradation nor to direct binding to the mRNA, and is reversible by dialysis or add-back of zinc, but not of other divalent metals, at the beginning of the reaction. These findings suggest that the activity of a putative zinc-dependent metalloprotein(s) involved in the second step of splicing is affected. This study outlines a new method for specific reversible inhibition of the second step of splicing using external reagents, and suggests a possible role of divalent cations in the second step of mRNA splicing, most likely zinc.
Rapid and sensitive trace gas detection with continuous wave Optical Parametric Oscillator-based Wavelength Modulation Spectroscopy (2010)

A fiber-amplified Distributed Bragg Reflector diode laser is used to pump a continuous wave, singly resonant Optical Parametric Oscillator (OPO). The output radiation covers the 3–4 μm with ability of rapid (100 THz/s) and broad mode-hop-free tuning (5 cm−1). Wavelength Modulation Spectroscopy is combined with the OPO to take optimal advantage of the spectral scan speed. The sensitivity of the system was determined as 0.8 ppbv (parts-per-billion by volume) for ethane (C2H6) for the absorption peak at 2996.9 cm−1 recorded in 1.3 seconds, corresponding to a noise equivalent absorption sensitivity (NEAS) of 1.2×10−9 cm−1/Hz1/2. A comparison between results using the 1st, 2nd and 4th harmonic derivative signal from wavelength modulation was performed. The broad continuous tunability was demonstrated by covering 35 cm−1 while recording absorption features of ethane, methane and water.
Nouvelles découvertes archéologiques relatives à Edesse (1961)

Leroy Jules. Nouvelles découvertes archéologiques relatives à Edesse. In: Syria. Tome 38 fascicule 1-2, 1961. pp. 159-169.