gebhart/patrepeval huggingface dataset:
Specific DNA Probe for the Diagnosis of <i>Plasmodium falciparum</i> Malaria (1986)

Malaria can be diagnosed either by direct microscopic examination of blood smears, which is time consuming and requires expertise, or by immunological techniques, which are effective but do not distinguish between past and present infections. In this study, a simple procedure was developed for spotting lysed blood from infected patients directly onto nitrocellulose paper and identifying the malaria species on the basis of hybridization of parasite DNA with a species-specific probe. A genomic DNA library of Plasmodium falciparum was screened to detect clones containing DNA sequences that are highly repeated within the parasite genome. Several such clones were further analyzed to identify those that hybridize specifically with P. falciparum DNA but not with DNA from humans, P. vivax, or P. cynomolgi. This technique appears to be sensitive enough to detect 10 picograms of purified P. falciparum DNA (equivalent to 100 parasites) and in field studies is able to detect approximately 40 parasites per microliter of blood.
Electronic structure and electron-phonon interactions in layered<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Li</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="italic">x</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">NbO</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msub></mml:mrow></mml:math>and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Na</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="italic">x</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">NbO</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msub></mml:mrow></mml:math> (1994)

Results of full-potential linear-muffin-tin-orbital calculations for the new intercalated niobium oxide phases ${\mathrm{Li}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$ and ${\mathrm{Na}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$ are presented. It is shown that alkali atoms are ionized, and their valence electrons completely fill the hybridized Nb d--O 2p conduction band in ${\mathit{M}}_{1.0}$${\mathrm{NbO}}_{2}$ crystals making them semiconducting. In the nonstoichiometric ${\mathit{M}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$ phases, the Fermi level is located well inside the conduction band of predominant Nb 4d nature with significant contributions from O 2p states. Estimates of electron-phonon interaction parameters and ${\mathit{T}}_{\mathit{c}}$ in the scope of the rigid-ion approximation result in ${\mathit{T}}_{\mathit{c}}$\ensuremath{\sim}12 K for ${\mathrm{Li}}_{0.5}$${\mathrm{NbO}}_{2}$ and ${\mathit{T}}_{\mathit{c}}$\ensuremath{\sim}9 K for ${\mathrm{Na}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$ in reasonable agreement with the experimental data---and show that superconductivity observed in these materials is of the usual electron-phonon type, similar to that in cubic ${\mathrm{Li}}_{0.5}$${\mathrm{TiO}}_{2}$. It is shown that the density of states at the Fermi level and ${\mathit{T}}_{\mathit{c}}$ for ${\mathrm{Na}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$ should be more critically dependent on stoichiometry than for ${\mathrm{Li}}_{\mathit{x}}$${\mathrm{NbO}}_{2}$. Since the electronic-structure peculiarities obtained in the calculations are very different than those typical for superconducting cuprates, it does not appear likely that any high-${\mathit{T}}_{\mathit{c}}$ superconductors should be expected in this class of compounds.
Elucidation of the Molecular Recognition of Bacterial Cell Wall by Modular Pneumococcal Phage Endolysin CPL-1 (2007)

Pneumococcal bacteriophage-encoded lysins are modular proteins that have been shown to act as enzymatic antimicrobial agents (enzybiotics) in treatment of streptococcal infections. The first x-ray crystal structures of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1, in complex with three bacterial cell wall peptidoglycan (PG) analogues are reported herein. The Cpl-1 structure is folded in two well defined modules, one responsible for anchoring to the pneumococcal cell wall and the other, a catalytic module, that hydrolyzes the PG. Conformational rearrangement of Tyr-127 is a critical event in molecular recognition of a stretch of five saccharide rings of the polymeric peptidoglycan (cell wall). The PG is bound at a stretch of the surface that is defined as the peptidoglycan-binding sites 1 and 2, the juncture of which catalysis takes place. The peptidoglycan-binding site 1 binds to a stretch of three saccharides of the peptidoglycan in a conformation essentially identical to that of the peptidoglycan in solution. In contrast, binding of two peptidoglycan saccharides at the peptidoglycan-binding site 2 introduces a kink into the solution structure of the peptidoglycan, en route to catalytic turnover. These findings provide the first structural evidence on recognition of the peptidoglycan and shed light on the discrete events of cell wall degradation by Cpl-1.
Incidence, Risk Factors, and Outcomes of Idiopathic Pneumonia Syndrome after Allogeneic Hematopoietic Cell Transplantation (2019)

Our current knowledge of idiopathic pneumonia syndrome (IPS) predates improved specificity in the diagnosis of IPS and advances in hematopoietic cell transplantation (HCT) and critical care practices. In this study, we describe and update the incidence, risk factors, and outcomes of IPS. We performed a retrospective cohort study of all adults who underwent allogeneic HCT at the Fred Hutchinson Cancer Research Center between 2006 and 2013 (n = 1829). IPS was defined using the National Heart, Lung, and Blood Institute consensus definition: multilobar airspace opacities on chest imaging, absence of lower respiratory tract infection, and hypoxemia. We described IPS incidence and mortality within 120 and 365 days after HCT. We examined conditioning intensity (nonmyeloablative versus myeloablative with high-dose total body irradiation [TBI] versus myeloablative with low-dose TBI) as an IPS risk factor in a time-to-event analysis using Cox models, controlled for age at transplant, HLA matching, stem cell source, and pretransplant Lung function Score (a combined measure of impairment in Forced Expiratory Volume in the first second (FEV<sub>1</sub>) and Diffusion capacity for carbon monoxide (DLCO)). Among 1829 HCT recipients, 67 fulfilled IPS criteria within 120 days (3.7%). Individuals who developed IPS were more likely to be black/non-Hispanic versus other racial groups and have severe pulmonary impairment but were otherwise similar to participants without IPS. In adjusted models, myeloablative conditioning with high-dose TBI was associated with increased risk of IPS (hazard ratio, 2.5; 95% confidence interval, 1.2 to 5.2). Thirty-one patients (46.3%) with IPS died within the first 120 days of HCT and 47 patients (70.1%) died within 365 days of HCT. In contrast, among the 1762 patients who did not acquire IPS in the first 120 days, 204 (11.6%) died within 120 days of HCT and 510 (29.9%) died within 365 days of HCT. Our findings suggest that although the incidence of IPS may be declining, it remains associated with post-transplant mortality. Future study should focus on early detection and identifying pathologic mediators of IPS to facilitate timely, targeted therapies for those most susceptible to lung injury post-HCT.
Phosphazene. XLVI. P‐Hydrogen‐cyclotriphosphazene (1972)

Abstract Aminodiphosphazene III gehen mit Phosphor(III)‐Verbindungen. die mindestens zwei nukleophil verdrängbare Reste enthalten, eine Cyclokondensation ein. Statt der dabei zu erwartenden Cyclotriphosphaza‐diene IV entstehen Cyclotriphosphaza‐triene V mit phosphorständigem Wasserstoff. Während spektroskopisch nur diese Form nachweisbar ist, wird in der elektrophilen Substitution des Wasserstoffs durch Methyljodid doch auch eine Gleichgewichtsbeteiligung von IV erkennbar. Die ungewöhnliche Begünstigung des PH‐Prototropen V läßt auf einen erhöhten Energiebeitrag der Phosphazenbindung im cyclisch alternierenden System, d. h. auf eine Extrastabilisierung durch die Ausbildung des Cyclotriphosphaza‐triens schließen.
Viscosity of molten sodium salt hydrates (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTViscosity of molten sodium salt hydratesS. K. Sharma, C. K. Jotshi, and Amrao SinghCite this: J. Chem. Eng. Data 1984, 29, 3, 245–246Publication Date (Print):July 1, 1984Publication History Published online1 May 2002Published inissue 1 July 1984https://pubs.acs.org/doi/10.1021/je00037a004https://doi.org/10.1021/je00037a004research-articleACS PublicationsRequest reuse permissionsArticle Views233Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Long wavelength VCSELs using AlAs/GaAs mirrors and strain-compensated quantum wells (2002)

We present a 1.53 /spl mu/m strain-compensated MQW VCSEL using wafer-fused AlAs/GaAs DBR mirrors. Under room temperature pulsed pumping, we measured excellent dynamic single mode characteristics, a low threshold current of 10 mA, and a linewidth of less than 0.1 nm.
A role for PVRL4-driven cell–cell interactions in tumorigenesis (2013)

During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. PVRL4 promotes anchorage-independence by driving cell-to-cell attachment and matrix-independent integrin β4/SHP-2/c-Src activation. Solid tumors frequently have copy number gains of the PVRL4 locus and some have focal amplifications. We demonstrate that the transformation of breast cancer cells is dependent on PVRL4. Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. DOI:http://dx.doi.org/10.7554/eLife.00358.001.
A case of ABO-incompatible blood transfusion treated by plasma exchange therapy and continuous hemodiafiltration (2018)

ABO-incompatible blood transfusion is potentially a life-threatening event. A 74-year-old type O Rh-positive male was accidentally transfused with 280 mL type B Rh-positive red blood cells during open right hemicolectomy, causing ABO-incompatible blood transfusion. Immediately after the transfusion, the patient experienced a hypotension episode followed by acute hemolytic reaction, disseminated intravascular coagulation and acute kidney injury. Plasma exchange therapy was performed to remove anti-B antibody and free hemoglobin because they caused acute hemolytic reaction, disseminated intravascular coagulation, and acute kidney injury. Free hemoglobin levels decreased from 13 to 2 mg/dL for 2 h. Continuous hemodiafiltration was used to stabilize hemodynamics. The patient was successfully treated for acute hemolytic reaction, disseminated intravascular coagulation, and acute kidney injury. Plasma exchange therapy and continuous hemodiafiltration are likely to be effective treatments for ABO-incompatible blood transfusion, and further studies are required to assess this effectiveness in future.
New efficient and accurate moment matching based model for crosstalk estimation in coupled RC trees (2002)

In this paper we provide a new efficient moment matching based model for the estimation of crosstalk in coupled RC tree networks. This model is formulated based on an exact and efficient linear order recursive algorithm for the moment computation of such networks with both lumped and distributed RC elements. The formulas used in the model are efficiently obtained by using the results of moment computation without any matrix operation and can fit for arbitrary input waveforms. Simulation results show it performs better than all existing formulas.
Code planning strategy for UMTS-FDD networks (2002)

In UMTS-FDD, neighbouring cells can be allocated with scrambling codes in numerous ways. These allocations, however, are not necessarily equivalent since they impose different processing requirements at the user equipment and they have different synchronisation performance. This paper tries to explore the differences among the various code planning configurations in terms of synchronisation error probability, worst case number of attempts until synchronisation acquisition, worst case required complexity and peak processing power requirements for several neighbouring cell lists with sizes from 6 to 32 cells. Based on the previous results, it provides some generic guidelines regarding the code planning strategy of the UMTS networks, which will enable the latter to operate optimally.
ChemInform Abstract: An Efficient Transformation to the Structure of (.+‐.)‐Supinidine. (1999)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Excimer Laser Photorefractive Keratectomy for Myopia: Six-Month Results (1991)

Six eyes of six patients with myopia between -4.00 and -8.00 diopters underwent 193 nm Argon/fluoride excimer laser photorefractive keratectomy as part of a Phase II-A FDA Study. Six months after surgery, four of the six eyes had a spherical equivalent refraction within +/- 1.00 D; five eyes saw 22/30 or better without correction. All corneas demonstrated a faint reticulated subepithelial haze most visible by sclerotic scatter illumination. There were no episodes of delayed epithelial healing or recurrent erosions, and patients had no loss of contrast sensitivity, visual acuity under glare testing, or corneal sensation at the 3-month or 6-month visit. In this limited clinical trial, the excimer laser was capable of changing the refractive power of the cornea for correction of myopia. Long-term follow up of these patients will be necessary to determine the safety and efficacy of the procedure.
Comparative Analysis of T Cell Imaging with Human Nuclear Reporter Genes (2015)

A comparison of different reporter gene-reporter probe systems for imaging of T cell number was performed, and the hNET/(18)F-MFBG PET reporter system was found to be the most sensitive and capable of detecting approximately 35-40 × 10(3) T cells at the site of T cell injection in the animal model.
Electrochemical Characterization of Solid-State Electric Double Layer Capacitors assembled with Sulfonated Poly(Ether Ether Ketone) (2006)

Abstract not Available.
Autologous hemopoietic stem cell transplantation in severe rheumatoid arthritis: a report from the EBMT and ABMTR. (2004)

Autologous HSCT is a relatively safe form of salvage treatment in severe, resistant RA. In these open label studies significant responses were achieved in most patients, with over 50% achieving an ACR 50 or more response at 12 months. Although the procedure is not curative, recurrent or persistent disease activity may be subsequently controlled in some patients with DMARD. Clinical trials are necessary to develop this approach in patients with aggressive disease who have failed conventional treatment including anti-tumor necrosis factor agents.
VLA-4 integrin mediates lymphocyte migration on the inducible endothelial cell ligand VCAM-1 and the extracellular matrix ligand fibronectin. (1993)

We herein tested the ability of lymphocytes to utilize the beta 1 integrin VLA-4 to mediate cell migration when adhering to its cytokine-inducible endothelial cell ligand VCAM-1 or its extracellular matrix ligand fibronectin. We used an in vitro system consisting of purified VCAM-1/Fc fusion protein or fibronectin immobilized on porous polycarbonate membranes to quantitatively measure the migration efficiency of an Epstein-Barr virus-transformed B cell line (SLA) and T lymphoblasts derived from normal donors. We found that both SLA cells and T lymphoblasts migrated across membranes coated with VCAM-1/Fc or fibronectin in a site density-dependent manner. Above and below an optimal site density of VCAM-1/Fc or fibronectin, the migration efficiency decreased. A 6-20-fold higher number of lymphocytes migrated across membranes coated with VCAM-1/Fc than with fibronectin. The differential migration efficiency is consistent with a higher number of adherent lymphocytes and a higher avidity of adhesion for VCAM-1/Fc than for fibronectin when the ligands were immobilized on plastic, and is independent of the activation state of the cells. These results demonstrated a stringent regulation of migratory response by cell adhesion strength and a delicate balance between stationary and migratory behaviors of a cell on the adhesive substrates. Like the beta 2 integrin LFA-1, VLA-4 may be a locomotive adhesion receptor which is involved in the transendothelial migration of lymphocytes and the infiltration of lymphocytes into lymphoid or peripheral tissues by binding to VCAM-1 and fibronectin.
Control of Grass and Sedge in Allegheny Hardwood Stands with Roundup-Residual Herbicide Tank Mixes (1990)

Abstract A small plot experiment was carried out to investigate the effect of forest floor disturbance on establishment of grasses and sedges and to test whether addition of a residual herbicide in tank mix with Roundup could reduce grass and sedge reinvasion. Seven residual herbicides, each at three rates of application, were tested against a Roundup control. Herbicides were Hyvar X (bromacil), Karmex (diuron), Racer (fluorochloridone) (R-40244)), Devrinol (napropamide), Surflan (oryzalin), Oust (sulfometuron methyl), and Sinbar (terbacil). Herbicides were applied on three dates of application at two northwestern Pennsylvania sites. Half of each plot was mechanically disturbed to simulate logging disturbance. Response data were collected for 2 or 3 years after treatment application. Soil disturbance was a significant factor in promoting emergence of grass and sedge; most germination occurred during the growing season immediately following treatment. Little grass or sedge emerged on undisturbed areas. In the second growing season following treatment, plants that did emerge grew to full size. Two of the tank mixes, Roundup plus Hyvar X and Roundup plus Karmex, gave less control of vegetation on the plot at the time of treatment than Roundup alone. Most Round-up-residual herbicide tank mixes reduced emergence and growth of new grass and sedge plants, regardless of application date; however, the amount and duration of control differed among herbicides. Roundup (1 # ai/ac) plus Surflan (2-4 # ai/ac) and Roundup plus Oust (0.09-0.19 # ai/ac) produced the best results reducing grass and sedge ground cover for at least 3 and 2 years, respectively. By use of Roundup-residual herbicide tank mixes and manipulation of cutting techniques, forest land managers can minimize the impact of grass and sedge on the forest regeneration process in Allegheny hardwood stands. North. J. Appl. For. 7:124-129, September 1990.
Dynamic hair capture using spacetime optimization (2014)

Dynamic hair strands have complex structures and experience intricate collisions and occlusion, posing significant challenges for high-quality reconstruction of their motions. We present a comprehensive dynamic hair capture system for reconstructing realistic hair motions from multiple synchronized video sequences. To recover hair strands' temporal correspondence, we propose a motion-path analysis algorithm that can robustly track local hair motions in input videos. To ensure the spatial and temporal coherence of the dynamic capture, we formulate the global hair reconstruction as a spacetime optimization problem solved iteratively. Demonstrated using a range of real-world hairstyles driven by different wind conditions and head motions, our approach is able to reconstruct complex hair dynamics matching closely with video recordings both in terms of geometry and motion details.
A universal perspective on vulnerability (2020)

The guidelines are directed at both businesses and governments. The United Nations Guidelines for Consumer Protection begin with an allusion to the intrinsic vulnerability of consumers vis-a-vis suppliers. The general orientation of the UNGCP is universal but with the recognition that some groups or some circumstances have additional needs. Universalising infrastructure access often requires pipes, wires and trenches to be laid out, a process which may take years to take practical effect. Protection from, for example, disconnection during certain times and for certain groups, can be a useful protection especially when it is universal. The policy dilemmas become less acute when access is universal. There are mechanisms to encourage it such as the European Union concept of Services of General Economic Interest, which can reward service providers for taking responsibility. The broad remit of the UN Guidelines indicates a concern with issues of social equality and services intended to provide universal safety nets.
A defective signal peptide in the maize high-lysine mutant floury 2. (1995)

The maize floury 2 (fl2) mutation enhances the lysine content of the grain, but the soft texture of the endosperm makes it unsuitable for commercial production. The mutant phenotype is linked with the appearance of a 24-kDa alpha-zein protein and increased synthesis of binding protein, both of which are associated with irregularly shaped protein bodies. We have cloned the gene encoding the 24-kDa protein and show that it is expressed as a 22-kDa alpha-zein with an uncleaved signal peptide. Comparison of the deduced N-terminal amino acid sequence of the 24-kDa alpha-zein protein with other alpha-zeins revealed an alanine to valine substitution at the C-terminal position of the signal peptide, a histidine insertion within the seventh alpha-helical repeat, and an alanine to threonine substitution with the same alpha-helical repeat of the protein. Structural defects associated with this alpha-zein explain many of the phenotypic effects of the fl2 mutation.