gebhart/patrepeval huggingface dataset:
Enamel Defects and Ameloblast-specific Expression in Enam Knock-out/lacZ Knock-in Mice (2008)

Enamelin is critical for proper dental enamel formation, and defects in the human enamelin gene cause autosomal dominant amelogenesis imperfecta. We used gene targeting to generate a knock-in mouse carrying a null allele of enamelin (Enam) that has a lacZ reporter gene replacing the Enam translation initiation site and gene sequences through exon 7. Correct targeting of the transgene was confirmed by Southern blotting and PCR analyses. No enamelin protein could be detected by Western blotting in the Enam-null mice. Histochemical 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal) staining demonstrated ameloblast-specific expression of enamelin. The enamel of the Enam(+/-) mice was nearly normal in the maxillary incisors, but the mandibular incisors were discolored and tended to wear rapidly where they contacted the maxillary incisors. The Enam(-/-) mice showed no true enamel. Radiography, microcomputed tomography, and light and scanning electron microscopy were used to document changes in the enamel of Enam(-/-) mice but did not discern any perturbations of bone, dentin, or any other tissue besides the enamel layer. Although a thick layer of enamel proteins covered normal-appearing dentin of unerupted teeth, von Kossa staining revealed almost a complete absence of mineral formation in this protein layer. However, a thin, highly irregular, mineralized crust covered the dentin on erupted teeth, apparently arising from the formation and fusion of small mineralization foci (calcospherites) in the deeper part of the accumulated enamel protein layer. These results demonstrate ameloblast-specific expression of enamelin and reveal that enamelin is essential for proper enamel matrix organization and mineralization.
A selective nanosensing probe for nitric oxide (2008)

Measurement of NO gas in exhaled human breath may be used to monitor oxidative stress and pulmonary diseases. Until now, only bulk, expensive, chemiluminescence-based NO monitors have been available to medicine. A nanosensing probe based on WO3 selectively detecting minute nitric oxide gas concentrations in the presence of interfering volatile compounds is presented. This is possible due to the chemical affinity of rhenium trioxide based phases to oxidizing gases. The NO nanoprobe is expected to lead to portable and affordable, noninvasive, single breath sampling, NO diagnostics.
Radiofrequency identification technology can standardize and document blood collections and transfusions (2007)

RF microchips can collect key data during blood collections, facilitate information transfer from the blood center to the hospital, and confirm recipient-blood unit matching at the bedside before transfusions.
Computing Mitered Offset Curves Based on Straight Skeletons (2015)

NC simulation emulates the CNC machine tool and the cutter move along its axis.It depicts the material removal to better visualize the machining process.Until now, its main goals are still confined to check an unproved NC program to avoid potential collisions, and to analyze undercuts and overcuts.In this work, by using the simulated output-the in-process model (IPM), and rebuilding a machining feature based model, applications of NC simulation are extended to establish the feedback link from CNC to CAM, which can automatically pass modifications of manufacturing engineers back through the digital chain.In cases when old CAD archives of parts are incompatible with new systems and machines, or are damaged or lost, the original CAD model is neither available nor usable, and the part program is the only data available, rebuilding the feature based model from the NC simulation is necessary.This paper proposes an approach for machining feature recognition from IPM.As per the IPM characteristics, the approach adopts a novel approach of curvature based region segmentation and valuated adjacency graph based feature recognition.
Conserved Intergenic Elements and DNA Methylation Cooperate to Regulate Transcription at the il17 Locus (2012)

Naive CD4(+) T cells can differentiate into distinct lineages with unique immune functions. The cytokines TGFβ and IL-6 promote the development of Th17 cells that produce IL-17, an inflammatory cytokine not expressed by other T helper lineages. To further understand how IL-17 production is controlled, we studied an ~120-kb genomic region containing the murine il17a and il17f genes and seven evolutionarily conserved, intergenic noncoding sequences. We show that the +28-kb noncoding sequence cooperates with STAT3, RORγt, and Runx1 to enhance transcription from both il17a and il17f promoters. This enhancer and both promoters exhibited Th17 lineage-specific DNA demethylation, accompanied by demethylation of lysine 27 of histone H3 (H3K27) and increased H3K4 methylation. Loss of DNA methylation tended to occur at STAT3 consensus elements, and we show that methylation of one of these elements in the il17a promoter directly inhibits STAT3 binding and transcriptional activity. These results demonstrate that TGFβ and IL-6 synergize to epigenetically poise the il17 loci for expression in Th17 cells, and suggest a general mechanism by which active STAT3 may be epigenetically excluded from STAT3-responsive genes in non-Th17 lineages.
Face recognition based on facial feature training (2009)

The detection and extraction of characteristic features of the human face are primordial tasks in any given approach for face recognition. Within this context, the present paper will present a comprehensive list of steps that can help obtain the major discriminative features of the human face with the abstraction of the fuzzy data that are influenced by several external factors, notably light conditions, which often disrupt the results obtained by classifiers for human face recognition. The approach proposed in this paper can be considered a potentially strong candidate for use in a variety of commercial and industrial applications, particularly those related to security. In fact, in addition to its usefulness in identification processes, this face recognition system is also of particular importance for those who are interested in search and navigation processes in online video masses. In essence, our process was based on a corpus that contained a huge number of faces acquired in different positions and various lighting conditions. The identification and classification of faces was achieved through the use of a neural network called the multi-layer perceptron (MLP), which is one of the most common networks used in this context.
Inferring ancestry from population genomic data and its applications (2014)

Ancestry inference is a frequently encountered problem and has many applications such as forensic analyses, genetic association studies, and personal genomics. The main goal of ancestry inference is to identify an individual's population of origin based on our knowledge of natural populations. Because both self-reported ancestry in humans or the sampling location of an organism can be inaccurate for this purpose, the use of genetic markers can facilitate accurate and reliable inference of an individual's ancestral origins. At a higher level, there are two different paradigms in ancestry inference: global ancestry inference which tries to compute the genome-wide average of the population contributions and local ancestry inference which tries to identify the regional ancestry of a genomic segment. In this mini review, I describe the numerous approaches that are currently available for both kinds of ancestry inference from population genomic datasets. I first describe the general ideas underlying such inference methods and their relationship to one another. Then, I describe practical applications in which inference of ancestry has proven useful. Lastly, I discuss challenges and directions for future research work in this area.
Prototyping QoS based architecture for power plant control applications (2002)

The goal of the project is to gain experience on QoS (quality of service) based distributed system for EDF's power control applications. EDF is the French power utility. QoS is related to time constraints in this case. We adopted a distributed system approach to build our execution platform. The first prototype uses a modified ChorusOS micro-kernel with a real-time inter-process communication facility based on an ATM network and supports a specific real-time Java Virtual Machine. Our second proposal attempts to build an equivalent architecture using off-the-shelf products. Reality constraints lead to a CoS (classification of service) based design. We show that there is no available off-the-shelf QoS or CoS based technology for real-time object oriented distributed applications.
The mitochondrial <scp>ATP</scp> synthase is a shared drug target for aging and dementia (2018)

Aging is a major driving force underlying dementia, such as that caused by Alzheimer's disease (AD). While the idea of targeting aging as a therapeutic strategy is not new, it remains unclear how closely aging and age-associated diseases are coupled at the molecular level. Here, we discover a novel molecular link between aging and dementia through the identification of the molecular target for the AD drug candidate J147. J147 was developed using a series of phenotypic screening assays mimicking disease toxicities associated with the aging brain. We have previously demonstrated the therapeutic efficacy of J147 in several mouse models of AD. Here, we identify the mitochondrial α-F<sub>1</sub> -ATP synthase (ATP5A) as a target for J147. By targeting ATP synthase, J147 causes an increase in intracellular calcium leading to sustained calcium/calmodulin-dependent protein kinase kinase β (CAMKK2)-dependent activation of the AMPK/mTOR pathway, a canonical longevity mechanism. Accordingly, modulation of mitochondrial processes by J147 prevents age-associated drift of the hippocampal transcriptome and plasma metabolome in mice and extends lifespan in drosophila. Our results link aging and age-associated dementia through ATP synthase, a molecular drug target that can potentially be exploited for the suppression of both. These findings demonstrate that novel screens for new AD drug candidates identify compounds that act on established aging pathways, suggesting an unexpectedly close molecular relationship between the two.
Signalling by Rho family proteins (1997)

Conference Article| August 01 1997 Signalling by Rho family proteins A. J. Ridley A. J. Ridley 1Ludwig Institute for Cancer Research, 91 Riding House Street, London W1P 8BT, U.K.2Department of Biochemistry and Molecular Biology, University College London, Gower Street, London, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1997) 25 (3): 1005–1010. https://doi.org/10.1042/bst0251005 Article history Received: March 18 1997 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation A. J. Ridley; Signalling by Rho family proteins. Biochem Soc Trans 1 August 1997; 25 (3): 1005–1010. doi: https://doi.org/10.1042/bst0251005 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: GDI, GDP-dissociation inhibitor, DH, Dbl homology, GAP, GTPase-activating protein, PIP2, phosphatidylinositol 4,5-bisphosphate, WASP, Wiskott-Aldrich syndrome protein © 1997 Biochemical Society1997 Article PDF first page preview Close Modal You do not currently have access to this content.
Luminescence in epitaxial GaN : Cd (1974)

Investigations on luminescent properties of good-quality GaN single-crystalline epitaxial layers at temperatures in the range 1.6–300 K are reported. The high-energy region (3.27–3.48 eV) at 1.6 K is dominated by different bound exciton transitions. Peaks are observed at 3.469, 3.454, 3.447, 3.400, 3.377, 3.355, and 3.287 eV at 1.6 K in this region; their possible origin is discussed. At higher temperatures (T &gt; 30 K) the free A-exciton emission is also clearly observed, and thus its position EAx=3.475±0.0005 eV at 1.6 K could be established (neglecting polariton effects) together with a value of 6.4±0.4 meV for the binding energy of an exciton to a neutral donor. The energy region 3.0–3.3 eV is dominated by the previously studied donor-acceptor pair spectra, which peak at 3.263±0.003 in all our good-quality samples (undoped and doped). From the assignment of a subsidiary high-energy peak as free-to-bound transitions, a value of 29±6 meV for the donor binding energy is experimentally determined, in good agreement with the theoretical effective mass value. The region below 3.0 eV is in Cd-doped material dominated by a broad band centered at 2.72 eV, unlike previous observations. This band is an envelope composed of several discrete series of emissions observable at low temperatures. The solubility of Cd in GaN appears to be rather low, and high-Ohmic single-crystalline material was not obtained, in contrast to our results from Zn-doping.
The tumor suppressive effect of angiotensin II type 1 receptor antagonist in a murine osteosarcoma model. (2011)

CV11974 is widely used to treat hypertension clinically and therefore may be a novel antiangiogenic therapy for osteosarcoma through blocking AT1R-mediated signaling.
T lymphocyte activation: a biological model of signal transduction. (1990)

Here, we review the current state of the art in the area of T lymphocyte signal transduction. This communication is not intended to present an exhaustive review of the relevant voluminous literature, but rather a critical overview and synthesis of the important findings in this field
Laboratory-prepared reagent for the Beckman Creatinine Analyzer. (1979)

Journal Article Laboratory-prepared reagent for the Beckman Creatinine Analyzer. Get access D R Jury, D R Jury Search for other works by this author on: Oxford Academic Google Scholar R Ward R Ward Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 25, Issue 9, 1 September 1979, Page 1674, https://doi.org/10.1093/clinchem/25.9.1674 Published: 01 September 1979
Periodic Global Broadcast Time Synchronization (PGB-TS) for TDMA Based Sensor Networks (2007)

Wireless sensor networks become a new alternative for satellite systems to sense fine grained small size subjects. Time synchronization is an important aspect of distributed systems including wireless sensor networks (WSNs). Traditional time synchronization methods are mostly optimized for scalability and precision. We propose a Periodic Global Broadcast Time Synchronization (PGB-TS) mechanism for TDMA based WSNs to maximize energy efficiency. PGB-TS assumes that the sink can send periodic broadcast to all sensor nodes. Sensor nodes receive the global reference broadcast and synchronize themselves to the sink PGB-TS estimates clock drift with linear regression and accepts receive error. Energy consumption of radio circuit of a sensor network is modeled analytically. Simulation results show that PGB-TS consumes significantly less energy at the expense of somewhat lowered precision. Unfortunately, because of its lower precision, energy consumption of a sensor network with PGB-TS increases with the increased number of children. To avoid the dependency in the number of children, we also propose to employ a new Data Indication Slot Mechanism (DISM) into PGB-TS. Beneficially, DISM prevents redundant checks at the expense of possible overhearing situations. Analytical model for the proposed PGB-TS with DISM is supported by the simulation results.
A self-aligned EPROM structure with superior data retention (1990)

A structure that exhibits superior data retention, compared to the conventional erasable programmable read-only memory (EPROM) cell, while still using phosphosilicate glass (PSG) passivation, is described. The nitrided self-aligned MOS (NIT-SAMOS) employs a thin layer of low-pressure chemical vapor deposition (LPCVD) nitride between the double-poly-gate structure and the poly-metal isolation dielectric, to reduce the possibility of contamination of the floating-gate area. Comparisons are made of EPROM data retention lifetimes, programmability, and UV erasability, and n- and p-channel device parameters.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Guidelines for performing single-platform absolute CD4+ T-cell determinations with CD45 gating for persons infected with human immunodeficiency virus. Centers for Disease Control and Prevention. (2003)

These guidelines were developed by CDC for laboratorians who perform immunophenotyping for detection and enumeration of CD4+ T-cells and other lymphocyte subsets in persons infected with human immunodeficiency virus (HIV). The guidelines describe single-platform technology (SPT), a process in which absolute counts of lymphocyte subsets are measured from a single tube by a single instrument. SPT incorporates internal calibrator beads of known quantity in the analysis of specimens by three- or four-color flow cytometry. With CD45 gating, the relative numbers of beads and lymphocyte subsets are enumerated, and their absolute numbers and percentage values are calculated. This report supplements previous recommendations published in 1997 (CDC. 1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus [HIV]. MMWR 1997;46[No. RR-2]) that describe dual-platform technology, a method in which absolute counts are derived from measurements obtained from two instruments--a flow cytometer and hematology analyzer. The new recommendations address concerns specific to the implementation of SPT as well as other general topics such as laboratory safety and specimen handling.
Targeting FSTL1 Prevents Tumor Bone Metastasis and Consequent Immune Dysfunction (2013)

Bone metastasis greatly deteriorates the quality of life in patients with cancer. Although mechanisms have been widely investigated, the relationship between cancer bone metastasis and antitumor immunity in the host has been much less studied. Here, we report a novel mechanism of bone metastasis mediated by FSTL1, a follistatin-like glycoprotein secreted by Snail(+) tumor cells, which metastasize frequently to bone. We found that FSTL1 plays a dual role in bone metastasis-in one way by mediating tumor cell invasion and bone tropism but also in a second way by expanding a population of pluripotent mesenchymal stem-like CD45(-)ALCAM(+) cells derived from bone marrow. CD45(-)ALCAM(+) cells induced bone metastasis de novo, but they also generated CD8(low) T cells with weak CTL activity in the periphery, which also promoted bone metastasis in an indirect manner. RNA interference-mediated attenuation of FSTL1 in tumor cells prevented bone metastasis along with the parallel increase in ALCAM(+) cells and CD8(low) T cells. These effects were accompanied by heightened antitumor immune responses in vitro and in vivo. In clinical specimens of advanced breast cancer, ALCAM(+) cells increased with FSTL1 positivity in tumor tissues, but not in adjacent normal tissues, consistent with a causal connection between these molecules. Our findings define FSTL1 as an attractive candidate therapeutic target to prevent or treat bone metastasis, which remains a major challenge in patients with cancer.
The potential of differential mobility analysis coupled to MS for the study of very large singly and multiply charged proteins and protein complexes in the gas phase (2006)

As previously demonstrated by the technique of gas-phase electrophoretic mobility molecular analyzer (GEMMA) introduced by Kaufman and colleagues, differential mobility analysis (DMA) of charge-reduced electrospray ions in the gas phase is a useful complement to MS for studying large proteins and their weakly bound complexes. Several limitations of GEMMA, the solutions for which have the potential to greatly improve its performance, are discussed here, including DMA resolution and transmission. A quantitative theory of charge reduction kinetics for dried multiply charged globular proteins at atmospheric pressures is also presented, showing that the charge reduction time must be carefully chosen to maximize a singly charged ion signal, while avoiding survival of contaminating multiply charged species. Because charge reduction limits the range of masses analyzable by MS, we also consider the potential of a parallel-plate DMA coupled in series to an MS for DMA-MS studies without charge reduction.
A Dual-Metal Gate Integration Process for CMOS With Sub-1-nm EOT&lt;tex&gt;$hbox HfO_2$&lt;/tex&gt;by Using HfN Replacement Gate (2004)

A replacement gate process employing a HfN dummy gate and sub-1-nm equivalent oxide thickness (EOT) HfO/sub 2/ gate dielectric is demonstrated. The excellent thermal stability of the HfN-HfO/sub 2/ gate stack enables its use in high temperature CMOS processes. The replacement of HfN with other metal gate materials with work functions adequate for n- and pMOS is facilitated by a high etch selectivity of HfN with respect to HfO/sub 2/, without any degradation to the EOT, gate leakage, or time-dependent dielectric breakdown characteristics of HfO/sub 2/. By replacing the HfN dummy gate with Ta and Ni in nMOS and pMOS devices, respectively, a work function difference of /spl sim/0.8 eV between nMOS and pMOS gate electrodes is achieved. This process could be applicable to sub-50-nm CMOS technology employing ultrathin HfO/sub 2/ gate dielectric.
Single-chip microcontrollers for switchgear control (1991)

A switchgear controller utilizing a microcontroller is described. The primary goal was to mimic the functions and performance of the generic controller board with a microcontroller board which will cause the power automation system to consume less power. The code for the microcontroller implementation on a development board has been developed, and the signal conditioning for the signals to the microcontroller has been designed and tested. The implementation of this decision system for data gathering, command control, fault detection, and circuit protection, when compared to other implementations, provides more functions and better response while reducing weight, volume, and power consumption.
Effect of ranitidine on the pharmacokinetics and pharmacodynamics of prasugrel and clopidogrel (2008)

Results from this study suggest that there is no significant drug-drug interaction between oral ranitidine therapy and concomitantly-administered prasugrel or clopidogrel.