gebhart/patrepeval huggingface dataset:
Fundamentos bioquímicos da hereditariedade - I natureza do material genético (1969)

INTRODUCAOO presente trabalho constitui uma re­visao sumaria dos principais aspectos bio­quimicos ligados diretamente a Genetica e esta moldado sobre uma serie de pales­tras proferidas pelo autor no Servico de Pesquisas e Experimentacao do Instituto Nacional do Câncer (Secao de Imunologia e Selecao de Animais de Laboratorio) du­rante os meses de agosto e setembro de 1966.Essencialmente, no estudo dos funda­mentos bioquimicos da hereditariedade, devem ser abordados tres aspectos prin­cipais, quais sejam:1) A natureza da substância responsa­vel pelo aparecimento e pela transmissao dos caracteres biologicos (estrutura e fun­cao do DNA ou material hereditario);2) O modo de transmissao desse mate­rial de um organismo a outro:a) A duplicacao do DNA;b) As mutacoes a nivel molecular;3) O modo de acao desse material, isto e, como o DNA promove o aparecimento das caracteristicas biologicas:a) As enzimas;b) O controle qualitativo da sinteseproteica;c) O controle quantitativo da sinteseproteica.E fornecida, no fim de cada uma das seis secoes (1, 2a, 2b, 3a, 3b e 3c), uma bibliografia de referencias, contendo obras ou artigos de revisao geral e artigos ori­ginais selecionados; no final do trabalho e dada uma bibliografia geral; tanto uma como outra servirao aos interessados para um aprofundamento teorico, complemen­tando os diversos assuntos expostos.Este trabalho e para “Alsoprangt die Natur in hoher, voller Erschein- ung, und sie zeiget, gereiht, Glieder an Glieder gestuft”. (Gcethe).
Drug Design Strategies for Targeting G-Protein-Coupled Receptors (2002)

G-protein-coupled receptors (GPCRs) form a large protein family that plays an important role in many physiological and pathophysiological processes. Since the sequencing of the human genome has revealed several hundred new members of this receptor family, many new opportunities for developing novel therapeutics have emerged. The increasing knowledge of GPCRs (biological target space) and their ligands (chemical ligand space) enables novel drug design strategies to accelerate the finding and optimization of GPCR leads: The crystal structure of rhodopsin provides the first three-dimensional GPCR information, which now supports homology modeling studies and structure-based drug design approaches within the GPCR target family. On the other hand, the classical ligand-based design approaches (for example, virtual screening, pharmacophore modeling, quantitative structure-activity relationship (QSAR)) are still powerful methods for lead finding and optimization. In addition, the cross-target analysis of GPCR ligands has revealed more and more common structural motifs and three-dimensional pharmacophores. Such GPCR privileged structural motifs have been successfully used by many pharmaceutical companies to design and synthesize combinatorial libraries, which are subsequently tested against novel GPCR targets for lead finding. In the near future structural biology and chemogenomics might allow the mapping of the ligand binding to the receptor. The linking of chemical and biological spaces will aid in generating lead-finding libraries, which are tailor-made for their respective receptor.
Isolation of Amidase-negative Mutants of Pseudomonas aeruginosa Using Glycollamide as a Selective Agent (1987)

A positive selection is described for isolating amidase-negative mutants from Pseudomonas aeruginosa strains. The method is based on the conversion, via amidase activity, of glycollamide to glycollate which is growth inhibitory. Three types of mutant were isolated on lactate medium containing glycollamide: (i) mutants in which amidase activity was reduced or absent; (ii) double mutants that were amidase-negative and resistant to glycollate inhibition of growth; and (iii) glycollate-resistant mutants. By raising glycollamide concentrations in the selection medium, amidase-negative mutants were obtained from strains producing altered amidases with low specific acetamidase and glycollamidase activities. Glycollamide has wider applicability than fluoroacetamide as a selective agent for obtaining amidase-negative mutants.
Unique Offshore Recovery System Dramatically Reduces Environmental Impact by Recycling Stimulation Proppant (2003)

Summary During the past 4 years, the use of multiple-zone proppant fractures has significantly improved production and the ultimate productive life of horizontal wells in the Valhall chalk field. A typical well is designed with up to 10 proppant-fractured zones. The stimulation technique involves pumping as much as 300,000 lb of proppant into each zone, and during this process, up to 70,000 lb of excess proppant may remain in the wellbore, which is cleaned out with coiled tubing. Because the material contains a resin coating to facilitate adhesion in the reservoir, disposal is restricted. This waste previously had to be collected offshore, placed in large bags, containerized, and shipped onshore for incineration—a practice that was costly, wasteful, and environmentally suspect. In 1997, engineering studies revealed that the properties of the waste material made it suitable for recycling and use in future operations with minimal impact on fracture performance. While reusing proppant has become an accepted practice with no noticeable effect on well productivity, logistically it had some limitations. The material still had to be collected offshore and transported onshore, where it was stored for several months before being reloaded into the stimulation vessel for reuse in the next fracture treatment. This represents storage problems and environmental exposure of these materials. Furthermore, with no unforeseen delays, this was a 24-hour, two-way trip. This paper describes the development and successful application of a unique system that allows the excess proppant to be collected, treated, and recycled on location. The authors will discuss the evolution of the recycling technique and discuss the stepchange improvement that allows the material to be immediately reused offshore using specialized vacuum equipment. Approximately 150,000 lb of proppant was successfully recovered and recycled from the first well. The technique is still evolving and is likely to become a standard operation on Valhall wells. The offshore collection and recycling technique has eliminated the logistical problems associated with the previous method and will in time reduce the total proppant costs, along with minimizing the associated health, safety, and environment (HSE) impact.
Induction Motors with Rotor Helical Motion (2012)

A demand for sophisticated motion control is steadily increasing in several advanced application fields, such as robotics, tooling machines, pick-and-place systems, etc. These kinds of applications require implementation of at least two or more conventional motors/actuators, often operating with different type of mechanical gear. Electric motors/actuators that are able directly perform complex motion (with multiple degrees of mechanical freedom – multi-DoMF) may provide appreciable benefits in terms of performances, volume, weight and cost.
Microarray-based determination of anti-inflammatory genes targeted by 6-(methylsulfinyl)hexyl isothiocyanate in macrophages (2009)

6-(Methylsulfinyl)hexyl isothiocyanate (6-MSITC) is a bioactive ingredient of wasabi [Wasabia japonica (Miq.) Matsumura], which is a popular pungent spice of Japan. To evaluate the anti-inflammatory function and underlying genes targeted by 6-MSITC, gene expression profiling through DNA microarray was performed in mouse macrophages. Among 22,050 oligonucleotides, the expression levels of 406 genes were increased by ≥3-fold in lipopolysaccharide (LPS)-activated RAW264 cells, 238 gene signals of which were attenuated by 6-MSITC (≥2-fold). Expression levels of 717 genes were decreased by ≥3-fold in LPS-activated cells, of which 336 gene signals were restored by 6-MSITC (≥2-fold). Utilizing group analysis, 206 genes affected by 6-MSITC with a ≥2-fold change were classified into 35 categories relating to biological processes (81), molecular functions (108) and signaling pathways (17). The genes were further categorized as 'defense, inflammatory response, cytokine activities and receptor activities' and some were confirmed by real-time polymerase chain reaction. Ingenuity pathway analysis further revealed that wasabi 6-MSITC regulated the relevant networks of chemokines, interleukins and interferons to exert its anti-inflammatory function.
New perspectives on the hydrolytic degradation of the organophosphorothioate insecticide chlorpyrifos (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew perspectives on the hydrolytic degradation of the organophosphorothioate insecticide chlorpyrifosDonald L. Macalady and N. Lee WolfeCite this: J. Agric. Food Chem. 1983, 31, 6, 1139–1147Publication Date (Print):November 1, 1983Publication History Published online1 May 2002Published inissue 1 November 1983https://pubs.acs.org/doi/10.1021/jf00120a003https://doi.org/10.1021/jf00120a003research-articleACS PublicationsRequest reuse permissionsArticle Views409Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
CEACAM1 (CD66a) mediates delay of spontaneous and Fas ligand-induced apoptosis in granulocytes (2005)

Granulocytes form the first and fastest line of defense against pathogenic infections. Their survival is limited by apoptosis, a process that is critical for the resolution of inflammation. Pro-apoptotic and pro-inflammatory cytokines, as well as several receptors, can alter the lifespan of granulocytes. Here we report that the carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1, CD66a) is involved in the regulation of granulocyte survival. Until now CEACAM1 is described to control cell proliferation, cell migration, tumor growth, angiogenesis and diverse leukocyte functions. However, very little is known about its role in granulocytes. We found that CEACAM1 expression in resting rat granulocytes is significantly higher than in other leukocyte subtypes. Stimulation led to a strongly increased CEACAM1 cell surface expression and to release of soluble CEACAM1. DNA fragmentation assays and annexin V staining revealed that binding of CEACAM1-specific antibodies, Fab fragments and soluble CEACAM1-Fc constructs to cell surface-expressed CEACAM1 causes a delay of spontaneous and Fas ligand (CD95L)-induced apoptosis. Tyrosine phosphorylation of CEACAM1-L, its association with SHP-1, the activation of Erk1/2 and caspase-3 appeared to be crucial for the CEACAM1-mediated anti-apoptotic effect. These findings provide evidence that CEACAM1 influences the resolution of inflammation by prolonging the survival of rat granulocytes.
Protective Efficacy of CAP18<sub>106–138</sub>–Immunoglobulin G in Sepsis (2003)

Naturally present antibacterial proteins play an important role in innate host defense. A synthetic peptide mimicking the C-terminal lipopolysaccharide (LPS)-binding domain of rabbit cathelicidin CAP18 was coupled to immunoglobulin (Ig) G to create CAP18(106-138)-IgG, a construct that, in concentrations equimolar to those of peptide alone, binds and neutralizes LPS and kills multiple gram-negative bacterial strains. The protective efficacy of CAP18(106-138)-IgG was evaluated in a model of cecal ligation and puncture in mice. A single intravenous administration of 20 mg/kg CAP18(106-138)-IgG protected against mortality, compared with sham-coupled IgG (P<.03). There was no protection offered by administration of equimolar peptide alone (P=.96). There was a trend toward protection in C3H/HeJ mice that are minimally sensitive to LPS (P=.06), suggesting that direct detoxification of LPS was not the only mechanism of protection. Chemical or genetic coupling of antimicrobial peptides to IgG may be a means of using these peptides to treat infections.
Self‐organization of human <scp>iPS</scp> cells into trophectoderm mimicking cysts induced by adhesion restriction using microstructured mesh scaffolds (2018)

Cellular dynamics leading to the formation of the trophectoderm in humans remain poorly understood owing to limited accessibility to human embryos for research into early human embryogenesis. Compared to animal models, organoids formed by self-organization of stem cells in vitro may provide better insights into differentiation and complex morphogenetic processes occurring during early human embryogenesis. Here we demonstrate that modulating the cell culture microenvironment alone can trigger self-organization of human induced pluripotent stem cells (hiPSCs) to yield trophectoderm-mimicking cysts without chemical induction. To modulate the adhesion microenvironment, we used the mesh culture technique recently developed by our group, which involves culturing hiPSCs on suspended micro-structured meshes with limited surface area for cell adhesion. We show that this adhesion-restriction strategy can trigger a two-stage self-organization of hiPSCs; first into stem cell sheets, which express pluripotency signatures until around day 8-10, then into spherical cysts following differentiation and self-organization of the sheet-forming cells. Detailed morphological analysis using immunofluorescence microscopy with both confocal and two-photon microscopes revealed the anatomy of the cysts as consisting of a squamous epithelial wall richly expressing E-cadherin and CDX2. We also confirmed that the cysts exhibit a polarized morphology with basal protrusions, which show migratory behavior when anchored. Together, our results point to the formation of cysts which morphologically resemble the trophectoderm at the late-stage blastocyst. Thus, the mesh culture microenvironment can initiate self-organization of hiPSCs into trophectoderm-mimicking cysts as organoids with potential application in the study of early embryogenesis and also in drug development.
A Live Storage Migration Mechanism over WAN for Relocatable Virtual Machine Services on Clouds (2009)

IaaS (Infrastructure-as-a-Service) is an emerging concept of cloud computing, which allows users to obtain hardware resources from virtualized data centers. Although many commercial IaaS clouds have recently been launched, dynamic virtual machine (VM) migration is not possible among service providers; users are locked into a particular provider, and cannot transparently relocate their VMs to another one for the best cost-effectiveness. In this paper, we propose an advanced storage access mechanism that strongly supports live VM migration over WAN. It rapidly relocates VM disks between source and destination sites with the minimum impact on I/O performance. The proposed mechanism addresses I/O consistency of virtual disks before/after migration, which is the major issue regarding wide-area live migration. The proposed mechanism works as a storage server of a block-level storage I/O protocol (e.g.,iSCSI and NBD). Two key techniques (on-demand fetching and background copying) move on-line virtual disks among remote sites, transparently and efficiently. Our prototype system works perfectly for Xen and KVM without any modification to them. Experiments showed the prototype system also worked successfully for an emulated WAN environment.
Soil management on the Carrington silt loam (1932)

The Carrington silt loam occurs extensively on the gently rolling uplands throughout northeastern central Iowa and in the eastern central counties. It is one of the more important soil types in the Iowan drift soil area. The map (fig. 1) shows its approximate occurrence.\nCrop yields are generally quite satisfactory on this soil, but some areas are not producing properly, and in many cases much larger yields per acre may be secured by the adoption of better methods of soil management. Suggestions have been offered in a previous publication* which if adopted will make this soil more productive and insure its permanent fertility. These suggestions have been incorporated in what is known as the Iowa system of soil management.
Electrocaloric and Barocaloric Effects in Some Ferroelectric Hydrosulfates and Triglycinesulfate (2012)

A comparative analysis of electrocaloric and barocaloric efficiency of order-disorder ferroelectrics Rbx(NH4)1-xHSO4 and TGS is performed. A good agreement was found between intensive electrocaloric effects measured experimentally and calculated using electric equation of state. Barocaloric effects were evaluated analyzing the experimental data on heat capacity and T-p phase diagrams. In some crystals under study the same adiabatic temperature change can be produced by low pressure and rather high electric field.
Electrical conductivity and equation-of-state study of warm dense copper: Measurements and quantum molecular dynamics calculations (2005)

Absolute measurements of the pressure and the electrical conductivity of expanded copper in the warm dense matter regime ($\ensuremath{\rho}=0.5--0.3\phantom{\rule{0.3em}{0ex}}\mathrm{g}∕{\mathrm{cm}}^{3}$ and $6000\phantom{\rule{0.3em}{0ex}}\mathrm{K}<T<30\phantom{\rule{0.2em}{0ex}}000\phantom{\rule{0.3em}{0ex}}\mathrm{K}$) are obtained in an isochoric plasma closed-vessel (EPI). Quantum molecular dynamics simulations are found to be in excellent agreement with the experimental results, allowing for a detailed interpretation of the optical conductivities. A shift in energy of the $4s\ensuremath{\rightarrow}4p$ atomic line is explained by the rise of ionization with temperature.
High power, high brightness 2 W (200 μm) and 3 W (500 μm) CW AlGaAs laser diode arrays with long lifetimes (1990)

Continuous-wave lifetimes in excess of ten thousand hours have been demonstrated for single aperture AlGaAs laser diode arrays with 200 μm and 500 μm aperture widths at power levels of 2 and 3 W, respectively.
Orthogonal colloidal quantum dot inks enable efficient multilayer optoelectronic devices (2020)

Surface ligands enable control over the dispersibility of colloidal quantum dots (CQDs) via steric and electrostatic stabilization. Today's device-grade CQD inks have consistently relied on highly polar solvents: this enables facile single-step deposition of multi-hundred-nanometer-thick CQD films; but it prevents the realization of CQD film stacks made up of CQDs having different compositions, since polar solvents redisperse underlying films. Here we introduce aromatic ligands to achieve process-orthogonal CQD inks, and enable thereby multifunctional multilayer CQD solids. We explore the effect of the anchoring group of the aromatic ligand on the solubility of CQD inks in weakly-polar solvents, and find that a judicious selection of the anchoring group induces a dipole that provides additional CQD-solvent interactions. This enables colloidal stability without relying on bulky insulating ligands. We showcase the benefit of this ink as the hole transport layer in CQD optoelectronics, achieving an external quantum efficiency of 84% at 1210 nm.
A Full-Field Forecasting Tool for the Combined Water/Miscible Gas Flood at Prudhoe Bay (1994)

ABSTRACT This paper describes COBRA a PC/wo rkst at ion based full-field forecasting tool for the Prudhoe Bay Water/Miscib!e Gas Injection Project. The paper discusses the models of field performance and operation that comprise COBRA. These models are: a solvent allocation model, a throughput based waterflood performance model that is coupled with an EOR performance model, a surface infrastructure model, and a pattern reconfiguration model. COBRA was used to evaluate Stage 1 of the waterflood/EOR infill expansion project in Prudhoe’s Northwest Fault Block (NWFB). COBRA was used to evaluate the various development options proposed in the NWFB infill project. COBRA determined the amount and timing of incremental reserves associated with different development alternatives in the active flood area. Compared to isolated evaluations, COBRA was able to incorporate the full field effects of solvent reallocation, pattern reconfiguration, and the effects of surface fluid handling constraints on the future production.
CURB-BASED STREET FLOOR EXTRACTION FROM MOBILE TERRESTRIAL LIDAR POINT CLOUD (2012)

Abstract. Mobile terrestrial laser scanners (MTLS) produce huge 3D point clouds describing the terrestrial surface, from which objects like different street furniture can be generated. Extraction and modelling of the street curb and the street floor from MTLS point clouds is important for many applications such as right-of-way asset inventory, road maintenance and city planning. The proposed pipeline for the curb and street floor extraction consists of a sequence of five steps: organizing the 3D point cloud and nearest neighbour search; 3D density-based segmentation to segment the ground; morphological analysis to refine out the ground segment; derivative of Gaussian filtering to detect the curb; solving the travelling salesman problem to form a closed polygon of the curb and point-inpolygon test to extract the street floor. Two mobile laser scanning datasets of different scenes are tested with the proposed pipeline. The results of the extracted curb and street floor are evaluated based on a truth data. The obtained detection rates for the extracted street floor for the datasets are 95% and 96.53%. This study presents a novel approach to the detection and extraction of the road curb and the street floor from unorganized 3D point clouds captured by MTLS. It utilizes only the 3D coordinates of the point cloud.
Automatic Tooth Segmentation Using Active Contour Without Edges (2006)

Automating the postmortem identification of deceased individuals based on dental characteristics is receiving increased attention especially with the large number of victims encountered in mass disasters. An automated dental identification system compares the teeth present in multiple digitized dental records in order to access their similarity. The primary step in such a system is the estimation of the contour of each tooth in order to permit efficient feature extraction. Extracting the contour of the teeth is a very challenging task and has received inadequate attention in the literature. In this paper, the task of teeth contour extraction is accomplished using active contour without edges. This technique is based on the intensity of the overall region of the tooth image and, therefore, does not necessitate the presence of a sharp boundary between teeth. Further, this technique can extract the region contour in the presence of additive noise and in the absence of well-defined image gradients. Experimental results indicate the benefits of the proposed approach.