gebhart/patrepeval huggingface dataset:
Induced Release of Cell Surface Protein Kinase Yields CK1- and CK2-like Enzymes in Tandem (1996)

Several types of cell exhibit cell surface protein kinase (ecto-PK) activities with Ser/Thr-specificity. Ecto-PK sharing certain characteristics of protein kinase CK2 can be detached from intact cells by interaction with exogenous substrates (Kübler, D., Pyerin, W., Burow, E., and Kinzel, V. (1983) Proc. Natl. Acad. Sci. U.S.A. 80, 4021-4025). However, a detailed molecular analysis of this ecto-PK was hampered by the vanishingly small amounts of labile enzyme protein obtained by substrate-inducible enzyme release. We now describe the stabilization and enrichment of released ecto-PK by precipitation with polyethylene glycol followed by affinity chromatography on heparin-agarose. Ecto-PK is shown to consist of two separate forms released in tandem, ecto-PK I and ecto-PK II. Comparison with cell homogenates as well as cell surface biotinylation experiments excluded contamination with intracellular PK. Purified ecto-PK I and ecto-PK II exhibit respectively selective phosphorylation of CK1- and CK2-specific peptide substrates, a complementary sensitivity to inhibitory agents and a differential use of the cosubstrates ATP and GTP. Ecto-PK I consists of a 40-kDa moiety; the ecto-PK II is an ensemble of three components of 43- and 40-kDa (catalytic subunits) and a noncatalytic 28-kDa subunit. In addition, components of the ecto-PK II react with CK2-specific antibodies. Further, comparative peptide mapping and the results of mass spectrometry in combination with assignment of amino acid sequences confirmed that ecto-PK II is closely related if not identical to the protein kinase CK2. Assays with intact cells that result in the phosphorylation of a variety of endogenous membrane proteins showed that both ecto-PKs participate, and further, certain ecto-PK substrates become preferentially labeled by one or another of the enzymes, whereas others are phosphorylated by both ecto-PK activities.
Abiotrophia defectiva Bleb-associated Endophthalmitis Confirmed With 16S Ribosomal RNA Sequencing (2013)

One recognized complication of trabeculectomy with visually devastating potential is blebitis. We present a case of a 74-year-old woman with a culture and polymerase chain reaction-positive Abiotrophia defectiva bleb-associated endophthalmitis. Abiotrophia defectiva is a rare but possible cause of endophthalmitis secondary to blebitis and should be considered in culture-negative cases. Prompt identification, hence directed eradication, of the causative organism in such visually threatening cases may be facilitated by requesting polymerase chain reaction and 16S ribosomal RNA sequencing.
Defects in methylthioadenosine phosphorylase are associated with but not responsible for methionine-dependent tumor cell growth. (2000)

A large proportion of human tumor-derived cell lines and primary tumor cells show methionine-dependent growth. This phenomenon refers to the ability of cells to grow in media containing methionine and the inability of cells to grow in media supplemented with methionine's precursor, homocysteine (Hcy). Methionine can be formed by two different pathways, the recycling pathway and the salvage pathway. To discover the basis for methionine-dependent growth, we have analyzed 12 tumor cell lines and 2 non-tumor-derived cell lines for defects in two key genes in different methionine synthetic pathways. We found little evidence that defects in methionine synthase expression or mutations in the MS gene are correlated with methionine-dependent growth. However, we did find a correlation between methionine-dependent growth and defects in expression of methylthioadenosine phosphorylase (MTAP), a key enzyme in the salvage pathway. Three of the four cell lines lacking detectable MTAP protein were unable to grow in Hcy-containing media, whereas all six of the MTAP-positive cell lines tested showed strong growth. However, when we introduced MTAP cDNA into MTAP-deficient MCF-7 cells, the resulting cell line was still defective in growth on Hcy, although it could now grow on the salvage pathway precursor methylthioadenosine. These findings indicate that salvage pathway defects are not causally related to methionine-dependent growth.
A low cost microcontroller compensated crystal oscillator (2002)

A Microcontroller Compensated Crystal Oscillator (MCXO) is described which employs varactor compensation using a high precision digital to analog converter (DAC) and analog to digital converter (ADC). A proven analog Temperature Compensated Crystal Oscillator (TCXO) design was enhanced by adding a state-of-the-art microcontroller subsystem. The resulting design maintains low cost while preserving the high density packaging of the parent TCXO design. Development is on going, with a currently achievable temperature stability of plus or minus 0.5 ppm from minus 40 to plus 85 degrees C using an AT cut crystal. MCXO's are calibrated after all packaging is completed, thus reducing manufacturing costs. Recalibration, to compensate for effects such as aging, can be performed at any time through one of the four device pins. The complete MCXO is assembled in a 1/spl times/1 inch footprint package. Typical power consumption is 125 milliwatts from a single 5 volt supply.
A CONVENIENT AND GENERAL SYNTHESIS OF ALKANE SULFINIC ACIDS (1984)

Abstract 2-(Alkylsulfonyl)benzothiazoles are reduced with sodium tetrahydroborate to give alkane sulfinic acids in good yield.
Effect of different doses of dexmedetomidine on microcirculation of rats with sepsis (2018)

Objective
To evaluate the effects of dexmedetomidine at different doses on the early microcirculatory system in septic rats.


Methods
A total of 48 Wistar rats were randomly divided into the cecal ligation and puncture (CLP) group, small-dose treatment group, medium-dose treatment group and large-dose treatment group, 12 rats in each group. The sepsis model was made by the CLP method, and dexmedetomidine was given respectively at the speed of 2.5 μg·kg-1·h-1, 5 μg·kg-1·h-1 and 10 μg·kg-1·h-1 to rats in small, medium and large-dose treatment groups through continuous tail vein pumping. The mean arterial pressure and heart rate at 1, 3, 5 h after CLP were recorded; arterial blood gas was measured at 3 h after CLP, including pH, partial pressure of carbon dioxide, partial pressure of oxygen, base excess in extracellular fluid, and levels of bicarbonate, total carbon dioxide, oxygen saturation and blood lactic acid; the death of rats in each group at 24 h was calculated.


Results
The heart rate and the level of mean arterial pressure in the four groups were significantly different (F=2.935, 3.862; P=0.005, 0.001). Compared with the CLP group, the mean arterial pressure of rats in small, medium and large-dose treatment groups decreased significantly at 1 h after operation (all P 0.05). However, the heart rate of rats in small, medium and large-dose treatment groups began to decrease at 3 h and 5 h after operation (all P 0.05]. Compared with those in the CLP group, the pH value, partial pressure of carbon dioxide, base excess in extracellular fluid, bicarbonate, total carbon dioxide and blood lactic acid of rats in the small, medium and large-dose treatment groups were significantly different (all P < 0.05), and the oxygen saturation was significantly lower in the medium and large-dose treatment groups than in the CLP group (all P < 0.05). In addition, compared with the large-dose treatment group, the total carbon dioxide content and oxygen saturation in the small-dose treatment group and base excess in extracellular fluid in the medium-dose treatment group were significantly different (all P < 0.05). The proportional mortality indicator of the four groups (11/1, 9/3, 3/9, 2/10) was statistically significantly different (χ2=17.658, P=0.001), and it was significantly lower in the medium and large-dose treatment groups than in the CLP and small-dose treatment groups (all P < 0.008).


Conclusions
The continuous infusion of dexmedetomidine into CLP sepsis rats within 3 hours can significantly improve the microcirculatory system, decrease the heart rate and stabilize the mean arterial pressure. The survival of CLP sepsis rats can be obviously improved with the increased dose of dexmedetomidine.


Key words: 
Shock, septic; Dexmedetomidine; Microcirculatory system
Optical regenerator for optical ring inter-working application (1999)

The usefulness of an optical regenerator using electroabsorption (EA) modulators was confirmed at 10 Gbit/s for an optical ring inter-working application. The regeneration performance in a dual ring configuration was experimentally evaluated by using two recirculating loops.
Synthesis of <i>N</i>-Arylhydroxylamines by Tellurium-Catalyzed Reduction of Aromatic Nitro Compounds (1986)

Abstract p-Substituted nitrobenzenes were readily reduced with sodium borohydride and a catalytic amount of tellurium to give the corresponding hydroxylamines in good yields.
A Case for Network-Attached Secure Disks (CMU-CS-96-142) (2018)

By providing direct data transfer between storage and client, network-attached storage devices have the potential to improve scalability (by removing the server as a bottleneck) and performance (through network striping and shorter data paths). Realizing the technology's full potential requires careful consideration across a wide range of file system, networking and security issues. To address these issues, this paper presents two new network-attached storage architectures. (1) Networked SCSI disks (NetSCSI) are network-attached storage devices with minimal changes from the familiar SCSI interface (2) Network-attached secure disks (NASD) are drives that support independent client access to drive provided object services. For both architectures, we present a sketch of repartitionings of distributed file system functionality, including a security framework whose strongest levels use tamper resistant processing in the disks to provide action authorization and data privacy even when the drive is in a physically insecure location. Using AFS and NFS, trace results suggest that NetSCSI can reduce file server load during a burst of AFS activity by a factor of about 2; for the NASD architecture, server load (during burst activity) can be reduced by a factor of about 4 for AFS and 10 for NFS.
Refractory oxides for high temperature MHD heaters (1980)

In a coal-burning MHD (magnetohydrodynamic) power plant, the use of a regenerative air heater is one option for producing the required high plasma temperatures. However, the thermal, mechanical, and chemical stresses that the MHD environment places on materials are enormous and candidate materials must be carefully tested. The results of such testing for a variety of materials studied in heater test facilities at both FluiDyne Engineering Corporation and at Montana State University are reported. The experimental program and test facilities for MHD heater development, the rationale for the choice of materials, and their performance (success or failure) under various environments are described. Results are analyzed and future materials needs are discussed in light of the observed effects.
Signals in abscission (2001)

Summary Abscission is the term used to describe the process of natural separation of organs from the parent plant. This may be part of the highly programmed development of a plant, or in response to environmental stress. It enables temperate plants to overwinter and hence survive, but in agricultural or horticultural environments premature abscission can lead to significant crop losses. Abscission is the culmination of changes in gene expression, which result in the loosening of adjacent cell walls within the zone and subsequent cell separation. For many years it has been recognized that the balance between the plant hormones ethylene and auxin determine where, and when, separation takes place. As we begin to understand the mechanisms by which plant growth regulator signals are perceived and transduced, we can begin to understand how the process of abscission itself may be induced and regulated. This review details what we know of the signals that lead to the differentiation of zone cells; the environmental signals that promote cell separation, and the possible intracellular signalling events that culminate in organ shedding. Contents Summary 323 I. Introduction 323 II. Differentiation of the abscission zone 324 III. Environmental and developmental signals that induce abscission 327 1. Senescence 327 2. Photoperiod 327 3. Water stress 328 4. Wounding and pathogen attack 328 5. Ozone 329 IV. Inter‐ and intracellular signals 329 1. Role of ethylene and auxin 330 2. Mechanisms of plant hormone signal perception 330 3. Roles of other hormones and components of signal transduction 333 V. Conclusions 335 Acknowledgements 336 References 336
Selective neuronal cell attachment to a covalently patterned monoamine on fluorinated ethylene propylene films (1993)

The patterned covalent surface addition of a monoamine to fluorinated ethylene propylene films (FEP) controls both cellular attachment and differentiation in defined media conditions. A radio frequency glow discharge (RFGD) process was used to replace FEP surface fluorine atoms with hydroxyl groups. The primary amine was then covalently attached by polymerizing aminopropyl-triethoxysilane (APTES) via the hydroxyl functionalities. The selective attachment of cells to the APTES regions was determined to be dependent upon the initial adsorption of albumin to the patterned FEP membrane. Albumin was determined to enhance cellular attachment to the APTES regions and prevent attachment to the unmodified FEP areas for both an NB2a neuroblastoma cell line and primary rat endothelial cells. If albumin were not preadsorbed onto the membrane, selective attachment to the modified regions would not occur. Radiolabeling albumin with 125I demonstrated the preference of albumin for adsorption onto the monoamine surface where the cells preferentially attached. Both hydrophobic and ionic forces contributed to the adsorption process. Although selective cellular attachment to the patterned APTES regions could be achieved by albumin preadsorption to the surface, the neuroblastoma cells did not significantly differentiate unless additional serum components were supplemented to the media.
Design and implementation of privacy-enhanced operation history middleware for smartphones (2011)

Operation history middleware collects various operation data on smartphones. Because of privacy concerns about sensitive operation data, it needs to meet fine-grained collection needs including collection based on temporal-spatial conditions and temporary suppression of the collection of specific operation types. The on-demand collection method proposed herein dynamically determines the minimum collection set of operation types and their storage period. We develop an Android-based prototype that implements five collection techniques and a selective encryption function to protect sensitive data in the local database. Our evaluation shows that the collection techniques incur low overhead. Encryption overhead in recording is less than 26 milliseconds.
Blood pressure estimation using video plethysmography (2016)

Remote sensing of vital physiological signs allows for unobtrusive, nonrestrictive and non-contact assessment of individual's health. By using video plethysmogram obtained by digital camera recordings of patient's face or hands, health parameters such as heart rate, respiratory rate and heart rate variability have already been investigated. In this paper, time-domain video plethysmogram from forehead was used for calculation of pulse transit time, which is related to blood pressure. Synchronous measurements of non-contact video plethysmogram, 12-channel electrocardiogram and invasive blood pressure were performed on three subjects. Our results demonstrate that pulse transit time method can be equally efficient with non-contact VPG signal, with error mean and standard deviation of 9.48 ± 7.13 mmHg and 4.48 ± 3.29 mmHg, for systolic and mean arterial pressure, respectively. Additionally our findings show that delay in pulse transit time, calculated from two different VPG signals from forehead and palm, could provide clinically useful measure of changes in systolic blood pressure.
Improved Handset Antenna Performance via an Electrically Extended Ground Plane (2012)

A novel and practical approach is presented providing improved antenna performance without enlarging the antenna or the ground plane. The approach electrically extends the ground plane using wire(s) that behave as surface metal extensions of the ground plane. The wire extensions can be accommodated within typical handset housing or as part of the stylish metal used on the handset’s exterior perimeter; hence don’t require enlargement of the device. Consequently, this approach avoids the costs and limitations traditionally associated with physically lengthening of a ground plane. Eight variations are presented and compared with baseline antenna performance. Both far-field patterns and near-field electromagnetic scans demonstrate that the proposed approach controls the electrical length of the ground plane and hence its chassis wavemodes, without negatively impacting the characteristics of the antenna. Improvements in performance of up to 56% in bandwidth at 900 MHz and up to 12% in efficiency with a reduction of up to 12% in the specific absorption rate (SAR) are achieved. An 8% increase in efficiency with a 1.3% improvement in bandwidth and a 20% reduction in SAR is achieved at 1880 MHz. Thus, improvements in bandwidth are achieved without compromising efficiency. Further, improvements at lower frequencies do not compromise performance at higher frequencies.
Identification of polymorphisms associated with hypertriglyceridemia and prolonged survival induced by bexarotene in treating non-small cell lung cancer. (2011)

We identified novel polymorphisms exhibiting significant association with bexarotene induced hypertriglyceridemia, implicating their potential in predicting bexarotene-improved survival response.
Activation of <i>α</i><sub>7</sub> nicotinic acetylcholine receptor promotes survival of spinal cord motoneurons (1997)

Spinal cord motoneurons (MNs) undergo a process of cell death during embryonic development and are the target of lethal acquired or inherited disorders, such as the amyotrophic lateral sclerosis. Therefore, the identification of mechanisms leading to MN survival is of crucial importance. Elevations in intracellular Ca2+ promote chicken MN survival during the embryonic period of naturally occurring cell death. We have recently demonstrated that the alpha7 nicotinic acetylcholine receptor (nAChR) mediates significant increases in free Ca2+ concentration at membrane potentials at which other pathways for Ca2+ influx are inactive. Although it is possible that Ca2+ influx through alpha7 nAChR promotes cell survival, the relation between alpha7 nAChR activation, cytosolic free Ca2+ and mammalian spinal cord MN survival has not been established. In the present study we have now demonstrated that Ca2+ influx through the alpha7-subunit is sufficient to rescue a significant number of cultured spinal cord MNs from programmed cell death induced by trophic factor deprivation. This is the first demonstration that neuronal nAChRs are involved in the regulation of MN survival.
DNA Chips: The Future of Biomarkers (2000)

DNA chips are small, solid supports such as microscope slides onto which thousands of cDNAs or oligonucleotides are arrayed, representing known genes or simply EST clones, or covering the entire sequence of a gene with all its possible mutations. Fluorescently labeled DNA or RNA extracted from tissues is hybridized to the array. Laser scanning of the chip permits quantitative evaluation of each individual complementary sequence present in the sample. DNA chip technology is currently being proposed for qualitative and quantitative applications, firstly for the detection of point mutations, small deletions and insertions in genes involved in human diseases or affected during cancer progression; secondly, to determine on a genome-wide basis the pattern of gene expression in tumors, as well as in a number of experimental situations. The extraordinary power of DNA chips will have a strong impact on medicine in the near future, both in the molecular characterization of tumors and genetic diseases and in drug discovery and evaluation. Quantitative applications will soon spread through all fields of biology.
Vision-based gesture recognition for human-computer interaction and mobile robot's freight ramp control (2010)

Aiming at the use of hand gestures for human- computer interaction, this paper presents a novel approach for hand gesture-based control of mobile robot's freight ramp. The research was mainly focused on solving some of the most important problems that current HRI (Human-Robot Interaction) systems fight with. Presenting a simple approach to recognizing gestures through image processing techniques and a single web camera, we address the problem of hand gestures recognition using motion detection and algorithm based on histograms, which makes it efficient in unconstrained environments, easy to implement and fast enough.
Interferometric system based on swept source-optical coherence tomography scheme applied to the measurement of distances of industrial interest (2016)

We used an interferometric technique based on typical optical coherence tomography (OCT) schemes for measuring distances of industrial interest. The system employed as a light source a tunable erbium-doped fiber laser of ∼20-pm bandwidth with a tuning range between 1520 and 1570 nm. It has a sufficiently long coherence length to enable long depth range imaging. A set of fiber Bragg gratings was used as a self-calibration method, which has the advantage of being a passive system that requires no additional electronic devices. The proposed configuration and the coherence length of the laser enlarge the range of maximum distances that can be measured with the common OCT configuration, maintaining a good axial resolution. A measuring range slightly >17 cm was determined. The system performance was evaluated by studying the repeatability and axial resolution of the results when the same optical path difference was measured. Additionally, the thickness of a semitransparent medium was also measured.