gebhart/patrepeval huggingface dataset:
Reaktionen mit Benzotrichlorid und Pentachloräthyl‐benzol (1964)

Abstract Benzotrichlorid und Halogen‐benzotrichloride lassen sich unter Erhaltung der CCl 3 ‐Gruppe gut nitrieren. Die gewonnenen Halogen‐nitro‐benzotrichloride werden zu Halogen‐nitro‐benzoylchloriden verseift. Durch Reduktion entstehen Halogen‐amino‐benzotrichloride; diese sind nur als Salze oder N‐substitutierte Verbindungen stabil. Trichlormethyl‐benzoylchloride geben über die Azide durch Curtius‐Abbau Trichlormethyl‐phenylisocyanate, aus denen Trichlormethyl‐phenyl‐substituierte Harnstoffe und Carbamate zugänglich sind. Aus Chlorameisensäuretolylestern können durch Chlorierung Chlorameisensäure‐[trichlormethyl‐phenylester] gewonnen werden, bei denen das Cl‐Atom des COCl‐Restes unter Erhaltung der CCl 3 ‐Gruppe austauschbar ist. — Ausgehend von 3‐Pentachloräthyl‐anilin, das auch in freier Form stabil ist, von 3‐Pentachloräthylphenol oder 4‐Pentachloräthyl‐benzoylchlorid, wurden Harnstoffe und Carbamate mit der CCl 3 — CCl 2 ‐Gruppierung dargestellt. — Pentachloräthyl‐benzolsulfonsäuren und Derivate sind nach den üblichen Methoden zugänglich. — Durch Halogenentzug mittels Zinkstaubs entstehen aus den Pentachloräthyl‐benzolen α.ß.ß‐Trichlor‐styrole, die sich wiederum nitrieren, reduzieren und zu Carbamaten umsetzen lassen.
Controlling Bone Morphogenetic Protein Diffusion and Bone Morphogenetic Protein-Stimulated Bone Growth Using Fibrin Glue (2006)

In Brief Study Design. An in vitro and in vivo study. Objective. To evaluate the ability of fibrin glue to limit diffusion of recombinant human bone morphogenetic protein (rhBMP)-2 and its ability to protect spinal nerves from rhBMP-2 stimulated bone growth. Summary of Background Data. Studies have shown bone morphogenetic protein (rhBMP-2) stimulated bone growth can encroach on the spinal canal and nerves, causing neural compression. More recently, rhBMP-2 use in the cervical spine has been associated with life-threatening swelling. Fibrin glue has been used as a biologic carrier but has not been evaluated for its ability to limit rhBMP-2. Methods. In phase 1 of the study, rhBMP-2 soaked absorbable collagen sponges (ACS) were encapsulated in fibrin glue and immediately incubated in physiologic lactated ringers solution at 38°C. Samples of solution were tested for rhBMP-2 concentration. In phase 2 of the study, rats were surgically treated with laminectomy and placement of rhBMP-2/ACS versus laminectomy and placement of fibrin glue before placement of rhBMP-2/ACS. After 8 weeks, animals were euthanized and imaged using micro-computerized tomography. Results. The diffusion study showed a significant limitation in rhBMP-2 diffusion when encapsulated in fibrin glue. The laminectomy study revealed blockage of bone formation by fibrin glue and protection of the spinal canal. Conclusions. Fibrin glue can limit the diffusion of rhBMP-2, and, thus, it can be used to help protect the spinal canal and nerve roots from rhBMP-2 stimulated bone growth. This study evaluates the ability of fibrin glue to limit the diffusion and bone forming effects of recombinant human bone morphogenetic protein (rhBMP)-2. An in vitro experiment encapsulating rhBMP-2 in fibrin glue showed that fibrin glue can slow the release of rhBMP-2, while an animal study showed that it can also protect the spinal canal and nerve roots from excessive bone growth.
Contribution of Sensory Feedback to the Generation of Extensor Activity During Walking in the Decerebrate Cat (1999)

In this investigation we have estimated the afferent contribution to the generation of activity in the knee and ankle extensor muscles during walking in decerebrate cats by loading and unloading extensor muscles, and by unilateral deafferentation of a hind leg. The total contribution of afferent feedback to extensor burst generation was estimated by allowing one hind leg to step into a hole in the treadmill belt on which the animal was walking. In the absence of ground support the level of activity in knee and ankle extensor muscles was reduced to approximately 70% of normal. Activity in the ankle extensors could be restored during the "foot-in-hole" trials by selectively resisting extension at the ankle. Thus feedback from proprioceptors in the ankle extensor muscles probably makes a large contribution to burst generation in these muscles during weight-bearing steps. Similarly, feedback from proprioceptors in knee extensor appears to contribute substantially to the activation of knee extensor muscles because unloading and loading these muscles, by lifting and dropping the hindquarters, strongly reduced and increased, respectively, the level of activity in the knee extensors. This conclusion was supported by the finding that partial deafferentation of one hind leg by transection of the L4-L6 dorsal roots reduced the level of activity in the knee extensors by approximately 50%, but did not noticeably influence the activity in ankle extensor muscles. However, extending the deafferentation to include the L7-S2 dorsal roots decreased the ankle extensor activity. We conclude that afferent feedback contributes to more than one-half of the input to knee and ankle extensor motoneurons during the stance phase of walking in decerebrate cats. The continuous contribution of afferent feedback to the generation of extensor activity could function to automatically adjust the intensity of activity to meet external demands.
Arrangement of cellular fibronectin in noncollagenous fibrils in human fibroblast cultures (1991)

The assembly of fibronectin into fibrils was examined by high-voltage immunoelectron microscopy in subconfluent cultures of ascorbate-treated human skin fibroblasts. Cells grown in the presence of ascorbic acid for 24, 48 or 72 h were labeled with Ist-9, a monoclonal antibody specific for the EIIIA site in fibronectin, and polyclonal antibodies to type I collagen. Cells were then labeled with goat anti-mouse IgG and goat anti-rabbit IgG coupled to 5 or 18 nm colloidal gold beads. Our results show that by 24 h, fibronectin is observed in fibrils in the extracellular matrix. The majority of fibronectin in fibrils does not co-localize with type I collagen. Morphometric analysis of the distance between EIIIA sites in fibronectin fibrils (less than 12 nm in diameter) show that the EIIIA sites appear to be spaced approximately 84 nm apart. The distance of 84 nm suggests that fibronectin is fully extended in fibrils and that the amino termini of adjacent fibronectin dimers overlap by 20 nm. As fibronectin fibrils become thicker, the average distance between EIIIA sites in fibronectin dimers decreases to 42 nm. This decrease in the distance between EIIIA sites may be due to a staggering of fibronectin dimers within the fibril as the fibril matures.
Projection-based motion compensation for gated coronary artery reconstruction from rotational x-ray angiograms (2008)

Three-dimensional reconstruction of coronary arteries can be performed during x-ray-guided interventions by gated reconstruction from a rotational coronary angiography sequence. Due to imperfect gating and cardiac or breathing motion, the heart's motion state might not be the same in all projections used for the reconstruction of one cardiac phase. The motion state inconsistency causes motion artefacts and degrades the reconstruction quality. These effects can be reduced by a projection-based 2D motion compensation method. Using maximum-intensity forward projections of an initial uncompensated reconstruction as reference, the projection data are transformed elastically to improve the consistency with respect to the heart's motion state. A fast iterative closest-point algorithm working on vessel centrelines is employed for estimating the optimum transformation. Motion compensation is carried out prior to and independently from a final reconstruction. The motion compensation improves the accuracy of reconstructed vessel radii and the image contrast in a software phantom study. Reconstructions of human clinical cases are presented, in which the motion compensation substantially reduces motion blur and improves contrast and visibility of the coronary arteries.
Concurrent log-structured memory for many-core key-value stores (2017)

Key-value stores are an important tool in managing and accessing large in-memory data sets. As many applications benefit from having as much of their working state fit into main memory, an important design of the memory management of modern key-value stores is the use of log-structured approaches, enabling efficient use of the memory capacity, by compacting objects to avoid fragmented states. However, with the emergence of thousand-core and peta-byte memory platforms (DRAM or future storage-class memories) log-structured designs struggle to scale, preventing parallel applications from exploiting the full capabilities of the hardware: careful coordination is required for background activities (compacting and organizing memory) to remain asynchronous with respect to the use of the interface, and for insertion operations to avoid contending for centralized resources such as the log head and memory pools. In this work, we present the design of a log-structured key-value store called Nibble that incorporates a multi-head log for supporting concurrent writes, a novel distributed epoch mechanism for scalable memory reclamation, and an optimistic concurrency index. We implement Nibble in the Rust language in ca. 4000 lines of code, and evaluate it across a variety of data-serving workloads on a 240-core cache-coherent server. Our measurements show Nibble scales linearly in uniform YCSB workloads, matching competitive non-log-structured key-value stores for write- dominated traces at 50 million operations per second on 1 TiB-sized working sets. Our memory analysis shows Nibble is efficient, requiring less than 10% additional capacity, whereas memory use by non-log-structured key-value store designs may be as high as 2x.
Requirement for Cross-Linkage in the Stimulation of Transformation of Rabbit Peripheral Lymphocytes by Antiglobulin Reagents (1970)

Abstract Fab′ fragments of goat anti-rabbit fragment Fab and anti-rabbit fragment Fc were ineffective in the stimulation of transformation of rabbit peripheral lymphocytes. F(ab′)2 fragments had stimulatory activity comparable to that of the intact IgG antibody. Combination of Fab′ fragments of both goat anti-rabbit Fc and antirabbit Fab with the lymphocytes was demonstrated by their capacity to inhibit specifically transformation by the corresponding, intact antibody; in addition, transformation was observed when rabbit lymphocytes, pretreated with univalent fragments of goat anti-rabbit Fc, were subsequently allowed to react with bivalent antibody directed to the univalent fragments. These results indicate that the triggering of transformation by antiglobulin reagents is accomplished through crosslinkage of membrane constituents. Such a mechanism may be relevant to the stimulation of sensitive lymphocytes by antigen and thus may aid in providing a basis for understanding the mechanism of induction and paralysis in the immune response.
Measurement of small rotations by eigenvector flips in a quasi-isotropic Fabry–Perot (1987)

A novel small rotation or small birefringence measurement scheme based on the dynamics of the helicoidal eigenstates of a passive Fabry–Perot cavity with two quarter-wave plates is described. When the system is inserted between two crossed polarizers, the sensitivity of the method is shown to be proportional to the square of the finesse of the cavity. For a finesse of 50, the observed noise floor corresponds to an estimation of the minimum detectable small rotation of 3×10−8 rd. We discuss possible improvements and potential possibilities of the method.
Detection of sparse signals under finite-alphabet constraints (2009)

In this paper, we solve the problem of detecting the entries of a sparse finite-alphabet signal from a limited amount of data, for instance obtained by compressive sampling. While existing methods either rely on the sparsity property, the finite-alphabet property, or none of those properties to solve the under-determined system of linear equations, we capitalize on both the sparsity and the finite-alphabet features of the signal. The problem is first formulated in a Bayesian framework to incorporate the prior knowledge of sparsity, which is then shown to be solvable using sphere decoding (SD) or semi-definite relaxation (SDR) for efficient Boolean programming. A few toy simulations show how our method can outperform existing works.
Preexposure of macrophages to low doses of lipopolysaccharide inhibits the expression of tumor necrosis factor-alpha mRNA but not of IL-1 beta mRNA (1991)

LPS is known to be a potent activator of macrophages and induces the production of TNF-alpha and IL-1. However, the signaling events and regulatory mechanisms required for the activation of macrophages by LPS have not been resolved precisely. We show that LPS modulates its own response in macrophages. Proteose peptone-induced murine peritoneal macrophages (P-PEM) produce significant amount of TNF-alpha and IL-1 after stimulation with LPS. However, preexposure of macrophages to low doses (less than 1 ng/ml) of LPS renders them refractory to stimulation by a second round of LPS, as evaluated by production of TNF-alpha. The loss of sensitivity to a second round of LPS was selective for TNF-alpha production as the LPS-primed macrophages retained the ability to produce IL-1. Northern blot analysis was performed with total RNA obtained from control and LPS- (1 ng/ml) primed P-PEM after 3-h stimulation with a second round of LPS. The expression of TNF-alpha mRNA was inhibited in LPS-primed P-PEM, whereas the expression of IL-1 beta mRNA was the same in control and LPS-primed P-PEM, consistent with the data of biologic activities of these two cytokines. Zymosan-induced TNF-alpha production was the same in control and LPS-primed macrophages, indicating that not all of the pathways required for TNF-alpha production were affected by LPS priming. Monokines such as human (h) rIL-1 alpha, hrTNF-alpha, hrIL-6, and murine rIFN-beta could not substitute for the action of low doses of LPS, and addition of indomethacin could not restore TNF-alpha production. These results suggest that exposure of macrophages to low doses of LPS suppresses the production of TNF-alpha, but not of IL-1, by inhibiting the expression of mRNA through a noncyclooxygenase-dependent mechanism. Thus, LPS-induced production of TNF-alpha and IL-1 in macrophages are differently regulated.
The Effect of Estrogen and, Sex-Steroids and Thyroid Hormone Preparation on Bone Mineral Density in Senile Osteoporosis (1991)

In order to assess the effect of sex steroids on bone mineral density in Japanese with senile osteoporosis, the bone mineral density in 1/3 distal site of radius was measured serially before and after treatment for 2 years using single photon absorptiometry. Sixty seven old females with senile osteoporosis were divided into 4 groups, Group 1 (n = 28, mean age; 74.4 +/- 1.3 y.o., mean +/- SEM) was the control group, Group 2 (n = 14, mean age; 73.7 +/- 1.7 y.o.) was treated with 0.5-1.0 micrograms/day of 1 alpha -OHD3, Group 3 (n = 12, mean age; 75.4 +/- 2.9 y.o.) was treated with conjugated estrogen (Premarin) in a dose of 0.3125 mg/day (3 approximately 4 weeks administration followed by 1 week rest) and Group 4 (n = 13, mean age; 76.4 +/- 1.8 y.o.) was treated with sex-steroids (pregnenolone : androstenedione : androstenediol : testosterone : estrone = 1.0 mg : 1.0 mg : 0.5 mg : 0.1 mg : 5 micrograms/tablet) and thyroid hormone (thyroid-sicca 7.5 mg/tablet) preparation in a dose of 2 tablets/day. When the radial bone mineral density (RMD) before the treatment was taken as 100%, RMDs of each group at 6, 12, 18 and 24 months were 96.4 +/- 3.1%, 97.3 +/- 2.0%, 93.7 +/- 2.1% and 96.1 +/- 1.8% in Group 1, 100.8 +/- 2.8%, 106.4 +/- 2.1%, 101.3 +/- 3.4% and 108.8 +/- 2.9% in Group 2, 103.0 +/- 2.8%, 106.2 +/- 3.5%, 105.9 +/- 4.3% and 100.2 +/- 4.7% in Group 3, 105.3 +/- 2.2%, 104.7 +/- 2.3%, 112.6 +/- 6.4% and 112.1 +/- 6.7% in Group 4, respectively. Therefore, significant increases in RMD were observed in Groups 2, 3 (transient) and 4 when compared with Group 1. In Group 3, serum level of parathyroid hormone (PTH) was significantly (p less than 0.05) increased from 0.28 +/- 0.03 ng/ml before the treatment to 0.55 +/- 0.15 ng/ml at 24 months after the treatment. In Group 2, transient (6 months after the treatment) but significant (p less than 0.01) increase in urinary Ca/Creatinine ratio from 0.15 +/- 0.04 to 0.20 +/- 0.03 was found. Serum A1-P activities in Group 4 was shown to increase transiently from 131 +/- 10 IU to 151 +/- 12 IU (p less than 0.05) at 6 months and to 158 +/- 13 IU (p less than 0.01) at 12 months followed by subsequent decrease to 135 +/- 6 IU at 18 months and 133 +/- 10 IU at 24 months after the treatment.(ABSTRACT TRUNCATED AT 400 WORDS)
Mimicking Nature‘S Nanocarrier: Synthetic Low-Density Lipoprotein-Like Nanoparticles for Cancer-Drug Delivery (2007)

Evaluation of: Nikanjam M, Blakely EA, Bjornstad KA, Shu X, Budinger TF, Forte TK: Synthetic nano-low density lipoprotein as targeted drug delivery vehicle for glioblastoma multiforme. Int. J. Pharm. 3287, 86–94 (2007) [1]. Low-density lipoproteins have long been recognized as a viable nanocarrier for targeted delivery of drug and imaging agents. Many groups have published promising initial findings; however, progress in this field has been impeded by the need to isolate low-density lipoproteins from fresh donor plasma. In a recent paper by Nikanjam and colleagues, synthetic low-density lipoprotein-like nanoparticles were prepared from commercial lipids and a bifunctional synthetic peptide containing the low-density lipoprotein receptor-binding domain and the lipid-binding motif. These particles were shown to behave similarly to native low-density lipoproteins and also to bind to the low-density lipoprotein receptor on cancer cells. Herein, we evaluate the utility of this novel delivery vehicle and discuss what role this technology may have in nanomedicine.
Candida Vegetations on Silicone Voice Prostheses (1986)

Deposits found on the esophageal surface of silicone voice prostheses (used in postlaryngectomy voice rehabilitation) are held responsible for malfunction of the valve mechanism. Cultures and scanning electron microscopic examinations proved these deposits to consist of Candida organisms growing not only on the surface of the prosthesis but also into the silicone material itself. It is remarkable that the patients are apparently not affected by these Candida organisms. Selective decontamination of the oropharynx and coating of the prosthesis with poly-methyl methacrylates, or impregnation with antimycotic agents or disinfectants, may prove effective methods in prolonging the life of the device.
Deposition of calcium carbonate in karst caves: role of bacteria in Stiffe's Cave (2001)

Bacteria make a significant contribution to the accumulation of carbonate in severa! natural habitats where large amounts of carbonates are deposited. However, the role played by microbial communities in speleothem formation (stalactites, stalagmites etc.) in caves is still unclear. In bacteria carbonate is formed by autotrophic pathways, which deplete CO2 from the environment, and by heterotrophic pathways, leading to active or passive precipitation. We isolated cultivable heterotrophic microbial strains, able to induce CaCO3 precipitation in vitro, from samples taken from speleothems in the galleries of Stiffe’s cave, L’Aquila, Italy. We found a large number of bacteria in the calcite formations (1 x 104 to 5 x 109 cells g-1). Microscopic examination, in laboratory conditions at different temperatures, showed that most of the isolates were able to form calcium carbonate microcrystals. The most crystalline precipitates were observed at 32°C. No precipitation was detected in un-inoculated controls media or in media that had been inoculated with autoclaved bacterial cells. X-ray diffraction (XRD) analysis showed that most of the carbonate crystals produced were calcite. Bacillus strains were the most common calcifying isolates collected from Stiffe’s Cave. Analysis of carbonate-solubilization capability revealed that the non-calcifying bacteria were carbonate solubilizers.
[Natural foci of viruses borne by Phlebotomus papatasi in the USSR according to a serologic study of the population]. (1979)

Seven hundred sixteen blood serum specimens from residents of presumable foci of phlebotomus fevers in Turkmenia, Tajikistan, Uzbekistan, Azerbaijan, and Moldavia were examined by the neutralization, complement fixation, hemagglutionation-inhibition and indirect immunofluorescence tests for the presence of antibody to viruses of the group of phlebotomus fevers (Sicilyan, Neapolitan, and Karimabad) and to rhabdovirus isfahan transmitted by phlebotomus papatasi. For the first time, antibody to Karimabad and Isfahan viruses were found in residents of the Central Asian republics. Antibody to Sicilyan and Neapolitan fevers were found in residents of all the republics examined. Data have been obtained indicating probable pathogenicity of Isfahan virus for man.
Digestion of peanut allergens Ara h 1, Ara h 2, Ara h 3, and Ara h 6: A comparative <i>in vitro</i> study and partial characterization of digestion‐resistant peptides (2010)

Abstract Scope : There are differences in stability to pepsin between the major allergens in peanut; however, data are from different reports using different digestion models. This study provides a comprehensive comparison of the digestibility of the major peanut allergens. Methods and results : Peanut allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6 were incubated with pepsin to mimic the effect of gastric digestion. Samples were analyzed using SDS‐PAGE. To further investigate resistance to digestion, Ara h 2 was additionally subjected to digestion with trypsin and residual peptides were characterized. Ara h 1 and Ara h 3 were rapidly hydrolyzed by pepsin. On the contrary, Ara h 2 and Ara h 6 were resistant to pepsin digestion, even at very high concentrations of pepsin. In fact, limited proteolysis could only be demonstrated by SDS‐PAGE performed under reducing conditions, indicating an important role for the disulfide bridges in maintaining the quaternary structure of Ara h 2 and Ara h 6. Trypsin digestion of Ara h 2 similarly resulted in large residual peptides and these were identified. Conclusion : Ara h 2 and Ara h 6 are considerably more stable towards digestion than Ara h 1 and Ara h 3.
Human Leucocyte Interferon: Analysis of Effect on MLC and Effector Cell Generation (1979)

Mixed lymphocyte cultures (MLC) showed decreased thymidine incorporation when interferon (IF) had been added to the culture medium. The cell-mediated lympholysis (CML) potential generated in mixed lymphocyte cultures grown in the presence of IF was substantially augmented at a certain concentration range. Highly purified leucocyte IF also had this effect, whereas mock preparations did not. The CML-augmenting property was found in the antiviral fraction after separation of semipurified leucocyte IF (by the method of Dahl & Degree). The possibility that the effects were due to a shift in kinetics after culturing in the presence of IF has been excluded. The killer-enhancing properties of IF did not seem to be due to an enhanced expression of histocompatibility antigens, the selective inhibition of CML suppressor cells, or a change in the specificity of recognized target cells. The results of secondary in vitro MLC-CML experiments, in which primary MLC had been carried out in the presence or absence of IF, indicated that the compound acts (in vitro) through preferential selection of cells that are less sensitive to inhibition by IF and more likely to kill.