gebhart/patrepeval huggingface dataset:
Cyanobacteriochrome CcaS regulates phycoerythrin accumulation in <i>Nostoc punctiforme</i> , a group II chromatic adapter (2010)

Responding to green and red light, certain cyanobacteria change the composition of their light-harvesting pigments, phycoerythrin (PE) and phycocyanin (PC). Although this phenomenon-complementary chromatic adaptation-is well known, the green light-sensing mechanism for PE accumulation is unclear. The filamentous cyanobacterium Nostoc punctiforme ATCC 29133 (N. punctiforme) regulates PE synthesis in response to green and red light (group II chromatic adaptation). We disrupted the green/red-perceiving histidine-kinase gene (ccaS) or the cognate response regulator gene (ccaR), which are clustered with several PE and PC genes (cpeC-cpcG2-cpeR1 operon) in N. punctiforme. Under green light, wild-type cells accumulated a significant amount of PE upon induction of cpeC-cpcG2-cpeR1 expression, whereas they accumulated little PE with suppression of cpeC-cpcG2-cpeR1 expression under red light. Under both green and red light, the ccaS mutant constitutively accumulated some PE with constitutively low cpeC-cpcG2-cpeR1 expression, whereas the ccaR mutant accumulated little PE with suppression of cpeC-cpcG2-cpeR1 expression. The results of an electrophoretic mobility shift assay suggest that CcaR binds to the promoter region of cpeC-cpcG2-cpeR1, which contains a conserved direct-repeat motif. Taken together, the results suggest that CcaS phosphorylates CcaR under green light and that phosphorylated CcaR then induces cpeC-cpcG2-cpeR1 expression, leading to PE accumulation. In contrast, CcaS probably represses cpeC-cpcG2-cpeR1 expression by dephosphorylation of CcaR under red light. We also found that the cpeB-cpeA operon is partially regulated by green and red light, suggesting that the green light-induced regulatory protein CpeR1 activates cpeB-cpeA expression together with constitutively induced CpeR2.
A New Prenylated Flavanone from<i>Erythrina suberosa</i>Roots (1987)

The roots of ERYTHRINA SUBEROSA were found to contain a new prenylated flavanone by combined physico-chemical studies.
Atomic Force Microscopy for Biological Imaging and Mechanical Testing across Length Scales (2010)

Atomic force microscopy (AFM) offers researchers a unique opportunity to visualize, manipulate, and quantitatively assess structural and mechanical aspects of native biological samples with nanometer resolution. An unparalleled advantage of AFM over other high-resolution microscopes is that biological specimens, ranging from tissues to cells to molecules, can be investigated in physiologically relevant aqueous environments. The AFM can be operated at 37°C, which makes it ideal for in situ cell or tissue studies. Combining an optical microscope with an AFM makes it possible to directly correlate structural/nanomechanical changes with optical/fluorescence images. This ability to simultaneously acquire structural and function information is unprecedented in biology. This article introduces the basics of AFM for imaging and investigating the properties of biological samples.
A multimedia control interface in teleoperation (2002)

The system described is used in teleoperated robotics to give the human operator a visual aid for the perception of the remote scene and for the robot command in case of indirect viewing via a video camera. The device consists of a camera which gives the operator an indirect view of the real world, sensors allowing the rebuilding of a virtual world. The superimposition of the real and virtual worlds is carried out onto a video display terminal. The system's functions are as follows: building graphics aids for the perception; building a geometric model of the environment; collecting data from the robot site to animate the computer picture of the environment model; matching computer-generated and video images; controlling the robot via the video display terminal.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Hand Gesture Recognition Using Blob Detection For Immersive Projection Display System (2012)

We developed a vision interface immersive projection system, CAVE in virtual rea using hand gesture recognition with computer vis background image was subtracted from current webcam and we convert the color space of the imag Then we mask skin regions using skin color range t a noise reduction operation. We made blobs fro gestures were recognized using these blobs. Using recognition, we could implement an effective bothering devices for CAVE. e framework for an reality research field vision techniques. ent image frame age into HSV space. e threshold and apply from the image and ing our hand gesture e interface without
Nucleation of Polystyrene Latex Particles in the Presence of <I>γ</I>-Methacryloxypropyltrimethoxysilane: Functionalized Silica Particles (2006)

Silica/polystyrene nanocomposite particles with different morphologies were synthesized through emulsion polymerization of styrene in the presence of silica particles previously modified by gamma-methacryloxypropyltrimethoxysilane (MPS). Grafting of the silane molecule was performed by direct addition of MPS to the aqueous silica suspension in the presence of an anionic surfactant under basic conditions. The MPS grafting density on the silica surface was determined using the depletion method and plotted against the initial MPS concentration. The influence of the MPS grafting density, the silica particles size and concentration and the nature of the surfactant on the polymerization kinetics and the particles morphology was investigated. When the polymerization was performed in the presence of an anionic surfactant, transmission electron microscopy images showed the formation of polymer spheres around silica for MPS grafting densities lower than typically 1 micromole x m(-2) while the conversion versus time curves indicated a strong acceleration effect under such conditions. In contrast, polymerizations performed in the presence of a larger amount of MPS moieties or in the presence of a non ionic emulsifier resulted in the formation of "excentered" core-shell morphologies and lower polymerization rates. The paper identifies the parameters that allow to control particles morphology and polymerization kinetics and describes the mechanism of formation of the nanocomposite colloids.
PROSPER: An Integrated Feature-Based Tool for Predicting Protease Substrate Cleavage Sites (2012)

The ability to catalytically cleave protein substrates after synthesis is fundamental for all forms of life. Accordingly, site-specific proteolysis is one of the most important post-translational modifications. The key to understanding the physiological role of a protease is to identify its natural substrate(s). Knowledge of the substrate specificity of a protease can dramatically improve our ability to predict its target protein substrates, but this information must be utilized in an effective manner in order to efficiently identify protein substrates by in silico approaches. To address this problem, we present PROSPER, an integrated feature-based server for in silico identification of protease substrates and their cleavage sites for twenty-four different proteases. PROSPER utilizes established specificity information for these proteases (derived from the MEROPS database) with a machine learning approach to predict protease cleavage sites by using different, but complementary sequence and structure characteristics. Features used by PROSPER include local amino acid sequence profile, predicted secondary structure, solvent accessibility and predicted native disorder. Thus, for proteases with known amino acid specificity, PROSPER provides a convenient, pre-prepared tool for use in identifying protein substrates for the enzymes. Systematic prediction analysis for the twenty-four proteases thus far included in the database revealed that the features we have included in the tool strongly improve performance in terms of cleavage site prediction, as evidenced by their contribution to performance improvement in terms of identifying known cleavage sites in substrates for these enzymes. In comparison with two state-of-the-art prediction tools, PoPS and SitePrediction, PROSPER achieves greater accuracy and coverage. To our knowledge, PROSPER is the first comprehensive server capable of predicting cleavage sites of multiple proteases within a single substrate sequence using machine learning techniques. It is freely available at http://lightning.med.monash.edu.au/PROSPER/.
Characterization of NVX‐207, a novel betulinic acid‐derived anti‐cancer compound (2009)

NVX-207 is well tolerated and has significant anti-cancer activity in vitro and in vivo in dogs with treatment-resistant malignancies.
Is there an entero‐hepatic circulation of the bile phospholipids? (1972)

Abstract Bile previously labeled with tritiated oleic acid (the main radioactivity was on bile phospholipids) was mixed with pure isolated phospholipids previously labeled with 14 C oleic acid; this mixture was perfused during 6 or 23 hr into the duodenum of test rats bearing a bile fistula. At the time of decapitation, in the small intestine a large hydrolysis of the 14 C phospholipids was found. In contrast no bile phospholipid hydrolysis was observed. In the collected bile samples of the test rats, no 14 C could be detected (this means a very large decrease of the 14 C fatty acids specific activities by the body fatty acids), and the tritiated fatty acids specific activities were only 2.5–12 times lower than in the perfused bile. These results can be explained, assuming that the bile phospholipids enter in an entero‐hepatic circulation and are preserved from the dilution in a large pool of lipids.
New insights into the role of the cytoskeleton in phagocytosis of Entamoeba histolytica (1999)

Entamoeba histolytica, a human parasite, crosses the natural barriers of the intestine and, in turn, spreads into the deeper organs, resulting in amoebiasis. The motility of the parasite and its ability to lyse or phagocytose human cells facilitates passage of the amoeba through the intestinal epithelium. Little is known about the uptake of material by this parasite; nevertheless, the cytoskeleton is believed to play a role in phagocytosis. Myosin IB, an actin-binding protein, localizes to the phagocytic cup and, with time, surrounds the internalized phagosome itself. The role of unconventional myosins in phagocytosis has also been demonstrated in other cell types, suggesting that this molecular mechanism is a common denominator in phagocytic events. Here, we summarize the emerging view of the role of unconventional myosins as well as other cytoskeleton-associated proteins in pseudopod formation at early stages of phagocytosis and during the late step of this process in E. histolytica.
Viable methodologies for the synthesis of high-quality nanostructures (2011)

The development of environmentally benign methods for the synthesis of nanomaterials has become increasingly relevant as chemists look to shape a more sustainable future. In this critical review, we present current work towards developing alternative methods for synthesizing a wide range of high-quality nanomaterials with predictable and controllable size, shape, composition, morphology and crystallinity. In particular, we focus on the inherent advantages of utilizing porous membrane templates, ultrasonic and microwave irradiation, alternative solvent systems, as well as biologically-inspired reagents as reasonably cost-effective, environmentally responsible methods to generate metal, metal oxide, fluoride, sulfide, selenide and phosphate nanomaterials.
Comparative affects of plasmid‐encoded interleukin 12 and interleukin 18 on the protective efficacy of DNA vaccination against <i>Mycobacterium tuberculosis</i> (2002)

Protective immunity against Mycobacterium tuberculosis infection requires the induction and maintenance of mycobacteria-specific, IFN-gamma-secreting CD4+ and CD8+ T lymphocytes. The development of Th1-like T cells is promoted by the early secretion and synergistic action of interleukin (IL)-12 and IL-18. This study compares the effects of plasmid-encoded IL-12 and IL-18 on the immunogenicity and protective efficacy of a DNA vaccine expressing the M. tuberculosis-secreted protein antigen 85B (DNA-85B). Co-immunization with either IL-12- or IL-18-expressing plasmids augmented the IFN-gamma-secreting T-cell response, and the maximum effect was observed with plasmids encoding both cytokines. Further the IL-12, but not the IL-18-expressing plasmid, significantly increased the protective efficacy of DNA-85B against pulmonary M. tuberculosis infection. Therefore co-administration of plasmid-encoded cytokines provides a potential method for optimizing the protective efficacy of DNA vaccination against tuberculosis.
Automated classification of patients with chronic lymphocytic leukemia and immunocytoma from flow cytometric three-color immunophenotypes (1997)

The goal of this study was the discrimination between chronic lymphocytic leukemia (B-CLL), clinically more aggressive lymphoplasmocytoid immunocytoma (LP-IC) and other low-grade non-Hodgkin's lymphomas (NHL) of the B-cell type by automated analysis of flow cytometric immunophenotypes CD45/14/20, CD4/8/3, kappa/CD19/5, lambda/CD19/5 and CD10/23/19 from peripheral blood and bone marrow aspirate leukocytes using the multiparameter classification program CLASSIF1. The immunophenotype list mode files were exhaustively evaluated by combined lymphocyte, monocyte, and granulocyte (LMG) analysis. The results were introduced into databases and automatically classified in a standardized way. The resulting triple matrix classifiers are laboratory and instrument independent, error tolerant, and robust in the classification of unknown test samples. Practically 100% correct individual patient classification was achievable, and most manually unclassifiable patients were unambiguously classified. It is of interest that the single lambda/CD19/5 antibody triplet provided practically the same information as the full set of the five antibody triplets. This demonstrates that standardized classification can be used to optimize immunophenotype panels. On-line classification of test samples is accessible on the Internet: http://www.biochem.mpg.de/valet/leukaem1.html Immunophenotype panels are usually devised for the detection of the frequency of abnormal cell populations. As shown by computer classification, most the highly discriminant information is, however, not contained in percentage frequency values of cell populations, but rather in total antibody binding, antibody binding ratios, and relative antibody surface density parameters of various lymphocyte, monocyte, and granulocyte cell populations.
Flexible Asymmetric Supercapacitors Based upon Co<sub>9</sub>S<sub>8</sub> Nanorod//Co<sub>3</sub>O<sub>4</sub>@RuO<sub>2</sub> Nanosheet Arrays on Carbon Cloth (2013)

We have successfully fabricated flexible asymmetric supercapacitors (ASCs) based on acicular Co9S8 nanorod arrays as positive materials and Co3O4@RuO2 nanosheet arrays as negative materials on woven carbon fabrics. Co9S8 nanorod arrays were synthesized by a hydrothermal sulfuration treatment of acicular Co3O4 nanorod arrays, while the RuO2 was directly deposited on the Co3O4 nanorod arrays. Carbon cloth was selected as both the substrate and the current collector for its good conductivity, high flexibility, good physical strength, and lightweight architecture. Both aqueous KOH solutions and polyvinyl alcohol (PVA)/KOH were employed as electrolyte for electrochemical measurements. The as-fabricated ASCs can be cycled reversibly in the range of 0-1.6 V and exhibit superior electrochemical performance with an energy density of 1.21 mWh/cm(3) at a power density of 13.29 W/cm(3) in aqueous electrolyte and an energy density of 1.44 mWh/cm(3) at the power density of 0.89 W/cm(3) in solid-state electrolyte, which are almost 10-fold higher than those reported in early ASC work. Moreover, they present excellent cycling performance at multirate currents and large currents after thousands of cycles. The high-performance nanostructured ASCs have significant potential applications in portable electronics and electrical vehicles.
Cyanuric Chloride‐Catalyzed Michael Addition of Indoles to Nitroolefins under Solvent‐Free Conditions (2012)

Cyanuric chloride is an inexpensive, efficient, and mild catalyst for the Michael addition of indoles to nitroolefins at 70°C under solvent‐free conditions. The simple experimental procedure, solvent‐free reaction conditions, utilization of an inexpensive and readily available catalyst, short period of conversion, and excellent yields are the advantages of the present method.
Secure and verifiable outsourcing protocol for non-negative matrix factorisation (2017)

With the rapid development of cloud computing services, resource-constrained clients can outsource their expensive computation tasks, such as scientific computations, to untrusted cloud servers. Furthermore, it is essential for these clients to protect their sensitive data and verify the validity of the returned computation results. In this paper, we focus on outsourcing protocol of non-negative matrix factorisation, which is an expensive computation task and has been widely applied to image processing, face recognition, text analysis, and so on. The permutation technique is employed to transform the original problem into a new one in our proposed protocol so as to protect the privacy, and the matrix 1-norm technique is utilised to verify the result returned from the cloud server in order to reduce the verification cost. Based on these two techniques, we construct a secure and verifiable outsourcing protocol for non-negative matrix factorisation. Moreover, the theoretical analysis and the experimental results show that our proposed protocol brings great computation savings for resource-constrained clients and fulfills the goals of correctness, security, verifiability and high efficiency.
The Preparation of Ethylene Imine from Monoethanolamine (1935)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation of Ethylene Imine from MonoethanolamineHenry WenkerCite this: J. Am. Chem. Soc. 1935, 57, 11, 2328Publication Date (Print):November 1, 1935Publication History Published online1 May 2002Published inissue 1 November 1935https://pubs.acs.org/doi/10.1021/ja01314a504https://doi.org/10.1021/ja01314a504research-articleACS PublicationsRequest reuse permissionsArticle Views1524Altmetric-Citations117LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
E-beam-patterned hydrogels to control nanoscale surface bioactivity (2005)

We are interested in controlling the spatial distribution of proteins on surfaces at cellular and subcellular length scales. To do this, we use a variation of e-beam lithography in a field-emission scanning electron microscope (SEM) to radiation crosslink thin films of water- soluble polymers such as poly(ethylene glycol) [PEG] and poly (carboxylic acids). We can simultaneously pattern the resulting hydrogels on silicon or glass surfaces with nanoscale and microscale feature sizes. Using hydroxy-terminated PEG 6800 we create gels with swell ratios between unity and fifteen depending on the degree of radiation crosslinking, and the swelling properties can be interpreted in terms of the Flory-Rehner formulation modified for one-dimensional swelling. While lightly-crosslinked PEG gels resist protein adsorption and cell adhesion as expected, highly crosslinked PEG gels adsorb such proteins as fibronectin and laminin and consequently become adhesive to fibroblasts, macrophages, and neurons. By spatially modulating the degree of crosslinking, we can localize these cells on surfaces and, for example, direct neurite outgrowth. If instead of using hydroxy-terminated PEG we use amine- terminated PEG, we introduce the additional flexibility of creating high-swelling PEG gels that resist nonspecific protein adsorption but to which specific proteins can be covalently bound. These can be surface patterned at submicron spacings, and we can pattern 7500 nanohydrogels in a 100 micron diameter arrays in 10 seconds. This is an areal density ~104 times greater than a modern DNA/protein chip, and the required bioreagents for chip fabrication and processing are proportionately less. We can bind fibronectin and laminin to different arrays, and we show that these proteins maintain their biospecificity after binding to the nanohydrogels with high fidelity. Looking to applications in next-generation protein-chip technology, our most recent experiments compare the performance of nanohydrogel arrays to that of standard protein microarrays using oligonucleotides which specifically bind nucleic acid-binding proteins.
A Comparison of Aristotle, Thomas Hobbes, and C.S. Lewis (2016)

In this paper, I will examine C.S. Lewis's Abolition of Man, which was written as a response to what Lewis perceived as a failure of modern culture to cherish the traditional values of morality and virtue.He observed that the education system was slowly eroding any idea of absolute truth, and was concerned with the implications of this.I will also look at Lewis' ideas through the lens of two prominent philosophers who came before him: Aristotle and Thomas Hobbes, and conclude with an excerpt from That Hideous Strength, a fictionalization of Abolition of Man.So, what exactly does Lewis mean when he uses the term "abolition of man"?In this text, he discusses how the chipping away of values and the dismissal of inherent good and evil are the beginning of this abolition.Lewis says that debunking traditional values will lead to the destruction of virtue.This, he says, is the result of a concerted effort to control the human body, therefore conditioning it to every whim and fancy of the conqueror.Indeed, control over the mind is the highest accomplishment one can attain when conquering nature.Lewis asks the following regarding the idea of controlling the human mind: "Why not this?Why must our conquest of nature stop short, in stupid reverence, before this final and toughest bit of 'nature' which has hitherto been called the conscience of man? ...
Coordination of Metal Cations with Amino-Functionalized MCM-41 for Adsorptive Desulfurization (2014)

Functionalized MCM-41 have been prepared with 3-aminopropyltrimethoxysilane (APTMS) as organic component and metal cations as inorganic component. The effects of different metal cations and concentration of APTMS on desulfurization performance for tert-butyl mercaptan (TBM) were investigated. The transmission electron microscopy (TEM) results indicate that the hexagonally ordered structure of MCM-41 remained intact after functionalization. The presence of grafted organosilanes was confirmed by fourier transform infrared (FTIR) spectra.