gebhart/patrepeval huggingface dataset:
Purification and characterization of a novel fibrinolytic enzyme from Streptomyces spp. (1999)

A novel protease with fibrinolytic activity, designated as SW-1, was isolated and purified from the fermentation broth of Streptomyces sp. strain Y405, a soil isolate. The purification procedure involved ammonium sulphate fractionation, decolorization on 290 resin, gel filtration on Sephadex G75, anion-exchange chromatography on DEAE Sephadex A25, and affinity chromatography on Lysine Sepharose 4B. About 4.2 mg purified enzyme was obtained from a liter of fermentation broth and the recovery yield was 12.0%. The purified enzyme showed the specific activity of 2952.3 urokinase units per milligram, which was increased by 230.6 fold over the fermentation broth. The purity determined by HPLC was 83.5%. SW-1 is a single chain polypeptide with a predicted molecular weight of 30 kDa in SDS-PAGE and an isoelectric point of 8.5. The N-terminal sequence of SW-1 is R/N/F-P/D-G-M-T-M-T-A-I-A-N-Q-N-T-Q-I-N. There may be nonhomogeneity in the first and second amino acid residue of its N-terminal sequence. The analysis of amino acid composition showed that SW-1 consisted of 262 amino acids. The fibrinolytic activity of SW-1 was entirely inhibited by 10 mmol/L PMSF, 1 mmol/L EDTA and 1 mol/L lysine, respectively, suggesting that SW-1 is a serine protease and metalloprotease, and that the lysine binding site might play a role in the activity. The fibrinolytic activity of SW-1 is stable between 4-37 degrees C and pH 4.0-9.0, and the optimum pH is 8.0. On plasminogen-free fibrin plates, SW-1 showed the same fibrinolytic activity as the mixture of SW-1 with plasminogen, indicating that SW-1 is a fibrinolytic enzyme which affects fibrin directly, but not a plasminogen activator which affects fibrin by activating plasminogen.
Integration of the <i>Tetrahymena</i> group I intron into bacterial rRNA by reverse splicing <i>in vivo</i> (1998)

Horizontal gene transfer is thought to contribute to the wide distribution of group I introns among organisms. Integration of an intron into foreign RNA or DNA by reverse self-splicing, followed by reverse transcription and recombination, could lead to its transposition. Reverse self-splicing of group I introns has been demonstrated in vitro, but not in vivo. Here we report RNA-dependent integration of the Tetrahymena intron into the 23S rRNA in Escherichia coli. Analysis of products by Northern blot and reverse transcription-PCR amplification revealed precise intron insertion into a site homologous to the natural splice junction. Products are sensitive to treatment with RNase but not DNase and depend on the splicing activity of the intron. Partial reaction with 11 novel sites in the 23S RNA that are complementary to the guide sequence of the intron illustrates lower specificity than intron homing. Reverse splicing of the Tetrahymena intron in bacteria demonstrates the possibility of RNA-catalyzed transposition of group I introns in foreign hosts.
General Method for the Synthesis of Ultrastable Core/Shell Quantum Dots by Aluminum Doping (2015)

Semiconductor quantum dots (QDs) have attracted extensive attention in various applications because of their unique optical and electronic properties. However, long-term photostability remains a challenge for their practical application. Here, we present a simple method to enhance the photostability of QDs against oxidation by doping aluminum into the shell of core/shell QDs. We demonstrate that Al in the coating shell can be oxidized to Al2O3, which can serve as a self-passivation layer on the surface of the core/shell QDs and effectively stop further photodegradation during long-term light irradiation. The prepared CdSe/CdS:Al QDs survived 24 h without significant degradation when they were subjected to intense illumination under LED light (450 nm, 0.35 W/cm(2)), whereas conventional CdSe/CdS QDs were bleached within 3 h.
Analysis of the <i>Lr34/Yr18</i> Rust Resistance Region in Wheat Germplasm (2008)

The Lr34/Yr18 adult plant resistance gene contributes significantly to durable leaf rust (caused by Puccinia triticina Eriks.) resistance. Simple and robust molecular markers that enable early detection of Lr34/Yr18 are a major advancement in wheat ( Triticum aestivum L.) breeding. An insertion/deletion size variant located at the csLV34 locus on chromosome 7D within an intron sequence of a sulfate transporter‐like gene tightly linked to the Lr34/Yr18 dual rust resistance gene was used to examine a global collection of wheat cultivars, landraces, and D genome–containing diploid and polyploid species of wheat relatives. Two predominant allelic size variants, csLV34a and b , found among the wheat cultivars showed disparate variation in different wheat growing zones. A strong association was observed between the presence of Lr34/Yr18 and the csLV34b allele and wheat lines known to have Lr34/Yr18 that had the csLV34a allele were rare. All landraces with the exception of those from China were predominantly of the csLV34a type. Only one size variant, csLV34a , was detected among the diploid and polyploid D genome–containing species, indicating that csLV34b arose subsequent to hexaploid bread wheat synthesis. The lineage of the csLV34b allele associated with Lr34/Yr18 in modern wheat cultivars from North and South America, CIMMYT, Australia, and Russia was tracked back to the cultivars Mentana and Ardito developed in Italy by Nazareno Strampelli in the early 1900s. The robustness of the csLV34 marker in postulating the likely occurrence of Lr34/Yr18 across a wide range of wheat germplasm and its utility in wheat breeding was confirmed.
Co-infection of Coronavirus Disease 2019 and Influenza A: A Report from Iran (2020)

This co-infection of COVID-19 and influenza A highlights the importance of considering SARS-CoV-2 PCR assay regardless of other positive findings for other pathogens in the primary test during the epidemic.
In <i>Saccharomyces cerevisiae</i> , Expression of Arginine Catabolic Genes <i>CAR1</i> and <i>CAR2</i> in Response to Exogenous Nitrogen Availability Is Mediated by the Ume6 (CargRI)-Sin3 (CargRII)-Rpd3 (CargRIII) Complex (2000)

The products of three genes named CARGRI, CARGRII, and CARGRIII were shown to repress the expression of CAR1 and CAR2 genes, involved in arginine catabolism. CARGRI is identical to UME6 and encodes a regulator of early meiotic genes. In this work we identify CARGRII as SIN3 and CARGRIII as RPD3. The associated gene products are components of a high-molecular-weight complex with histone deacetylase activity and are recruited by Ume6 to promoters containing a URS1 sequence. Sap30, another component of this complex, is also required to repress CAR1 expression. This histone deacetylase complex prevents the synthesis of the two arginine catabolic enzymes, arginase (CAR1) and ornithine transaminase (CAR2), as long as exogenous nitrogen is available. Upon nitrogen depletion, repression at URS1 is released and Ume6 interacts with ArgRI and ArgRII, two proteins involved in arginine-dependent activation of CAR1 and CAR2, leading to high levels of the two catabolic enzymes despite a low cytosolic arginine pool. Our data also show that the deletion of the UME6 gene impairs cell growth more strongly than the deletion of the SIN3 or RPD3 gene, especially in the Sigma1278b background.
Vulnerability ranking based on exploitation and defense graph (2010)

Network security analysis based on attack graphs has been applied extensively in recent years. The ranking of nodes in an attack graph is an important step towards analyzing network security, which can distill the overwhelming amount of information into a list of priorities that will help network administrators to efficiently utilize scarce resources. In this paper, we propose a new methodology called DBRank for ranking vulnerabilities to patch in computing networks. DBRank prioritizes vulnerabilities based on the diffusibility and benefit of vulnerability exploitation. Different from other approaches, DBRank takes into account the network topology and exploitation benefit in calculating their relative risk and priority. The experiments yielded promising results that this method can be used in hardening network security.
Effects Due to Absorption of Laser Radiation (1965)

This paper describes methods by which one may calculate the effects of high-power pulses of laser radiation of specified shapes absorbed at opaque surfaces. First the heating effects at levels where no phase change is produced are treated by ordinary thermodynamics. The vaporization produced by Q-switched lasers should proceed differently from that produced by ordinary lasers and the two processes are treated separately. The amount of material vaporized by an ordinary laser pulse is calculated using conventional methods, while we adopt a phenomenological model to treat the case of the higher peak power pulses from Q-switched lasers. The results are in agreement with experimental data.
Analysis and fabrication of corner cube array based on laser direct writing technology (2010)

A detailed analysis of corner cube array (CCA) structure is carried out using the commercially available ray-tracing software ZEMAX. The retroreflective properties of CCA for short-range and long-range propagation are compared. An improved CCA structure with a relatively low structural depth (compared with rectangular CCA) is designed and analyzed. Higher retroreflectance (compared with triangular CCA) at a specific incident angle is proved by ray tracing. Two kinds of CCA structures, including the improved CCA, have been fabricated using laser direct writing technology. The fabrication results are qualified by three-dimensional shape measurement and ray tracing.
Introduction (2005)

Japan's first unit of railway engineers was created in the wake of the first Sino-Japanese war (1894–95) when the Japanese army's supply lines serviced by horse-drawn cart proved too inefficient to support the six divisions in Manchuria. In 1900, one company of Japanese railway engineers took part in restoring and operating the badly damaged railway between Beijing and Tianjin during the Boxer rebellion, and the performance of the Japanese railway company was highly regarded by the allied armies. Japan's railway construction on the continent developed further during the early twentieth century as Japan's empire expanded. During the Russo-Japanese war (1904–5), one railway battalion composed of three companies moved to Korea and surveyed and supervised construction of a standard-gauge (1435 mm) railway between Seoul and Uiju on the Yalu river and then built a light railway from Antung (Andong) towards Mukden (Shenyang) following the advance of the Japanese army. The construction of the Antung railway through rugged terrain involved a work-force of three railway companies (800 men), two transport corps (400 men), 500 Japanese civilian craftsmen and about 1,000 local workers (coolies). By early February 1905, approximately 172 kilometres of track had been completed, close to the front line, and ammunition and food were forwarded by train eight times a day.KeywordsSouthern LineSupply DepotCommand GroupJapanese ArmyWooden BridgeThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Plasma concentrations of active simvastatin acid are increased by gemfibrozil (2000)

Gemfibrozil increases plasma concentrations of simvastatin and, in particular, its active form, simvastatin acid, suggesting that the increased risk of myopathy in combination treatment is, at least partially, of a pharmacokinetic origin. Because gemfibrozil does not inhibit CYP3A4 in vitro, the mechanism of the pharmacokinetic interaction is probably inhibition of non-CYP3A4-mediated metabolism of simvastatin acid.
Pentostatin, cyclophosphamide, and rituximab regimen in older patients with chronic lymphocytic leukemia (2007)

In this clinical trial, the PCR regimen was well tolerated by older patients and individuals with CrCl <or= 70. The efficacy of PCR was not significantly affected by age or renal function. These findings suggest PCR may be a good therapeutic option for older patients and those with modestly decreased renal function.
Data security-cryptography=privacy? (1992)

Concerns about the main encrypting and enciphering schemes being proposed as a way to ensure data security are examined. To elucidate the issues involved, public-key cryptography is explained. The collision between the interests of government intelligence agencies and private groups as evidenced by the controversy over the Digital Signature Standard (DSS) proposed by the US National Institute of Standards and Technology is discussed. Weaknesses of the DSS and possible curbs on cryptography are addressed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Arteriovenous Fistulas for Hemodialysis: Application of High-Frequency US to Assess Vein Wall Morphology for Cannulation Readiness (2011)

Venous IMT and hoop stress assessed with high-frequency US can predict cannulation readiness in AVFs that are clinically deemed mature.
Improved algorithms for hypergraph bipartitioning (2000)

Multilevel Fiduccia-Mattheyses MLFM hypergraph partitioning 3, 22, 24 is a fundamental optimization in VLSI CAD physical design. The leading implementation, hMetis 23 , has since 1997 pro veditself substantially superior in both runtime and solution quality t o e v en v ery recent w orks e.g., 13, 17, 25 . In this work, we present t w o sets of results: i new techniques for at FM-based hypergraph partitioning which is the core of multilev el implementations, and ii a new multilev el implementation that o ers leadingedge performance.
SAW sensor system application (2002)

The article gives an overview of the capabilities of sensor systems combining the basic principles of SAW identification (ID) tags with the properties of SAW sensors. The concept of SAW telemetry systems created in this way has several advantages compared with other solutions, with the two most important being that the sensors themselves do not require any separate energy source when operating, and that the SAW ID-sensor can provide the data in a remote, cordless manner. Different concepts for remote accessing and identifying SAW sensors via a wireless system are considered. Experimental results using a SAW temperature sensor with the remote sensing capability demonstrate the basic concept of this idea.
A DATABASE DESIGNED TO COMPUTATIONALLY AID AN EXPERIMENTAL APPROACH TO ALTERNATIVE SPLICING (2003)

A unique microarray approach has been developed to profile alternative splicing in the cell. To support the development of this approach, we have developed the Manually Annotated Alternatively Spliced Events (MAASE) database system, which is a unique alternative splicing information resource designed specifically with experimentalists in mind. MAASE is an online resource for the convenient access, identification, and annotation of alternative splicing events (ASEs). MAASE consists of two components: an annotation system and a curated database. The annotation system is a web-based workspace that combines manual and computational approaches to identifying and annotating ASEs, a combination that is vital if a comprehensive collection is to be obtained. The annotation system is publicly available and provides a scalable solution to acquiring as well as contributing to annotated ASEs. MAASE annotated ASEs are deposited into the database component, which can either be queried one entry at a time or multiple entries at a time with convenient access to alternatively spliced junctional and surrounding sequences to facilitate the design of microarray experiments.
Clinical research of reptilase combined with hypervolemic hemodilufion and controlled hypoteusion used in tumor surgery (2009)

Objective To investigate the safety and efficacy of reptilase combined with hypervolemic hemodilution and coutrolled hypotension used in tumor surgery. Methods 60 ASA Ⅰ - Ⅱ selected patients of tumor surgery were randomly allocated to three groups, group A: reptilase 2 000 U introvenous push before operation, 60 g/L HES(15 ml/kg) infused after induction of anesthesia, and nimodipine was used to maintain mean arterial pressure(MAP) between 60 mm Hg-65 mm Hg; group B, nimodipine was used to maintain MAP between 60 mm Hg-65 man Hg; group C, reptilase 2 000 U introvenous push before operation. HR, MAP, CVP, SpO2 and ECG were continuously monitored throughout operation. Arterial and central venous blood samples were taken for determination of Hb, Hct, PLT, FIB, APTT, PT, Posm and LA at T0 (baseline), T1 (onset of operation) , T2 (1 hour after operation) , T3 (completion of operation) respectively. The volume of blood loss and transfusion were recorded during operation in all patients. Results The value of Hb ,Hct in group A was obviously lower than that in goup B and in group C at T1 and the difference reached statistical significance(P 0. 05). The volume of blood loss and blood transfusion in group A were significantly less than that in goup B and C (P < 0.05 or P < 0.01). Conclusion Reptilase combined with hypervolemic hemodilution and controlled hypotensiou used in tumor surgery could effectively decrease the volume of blood loss during operation with relatively stable hemodynamics and mild side effects, and could be generalized in chnical use.

Key words: 
Reptilase;  Hemedilution;  Controlled hypotensiou;  Carcinoma
[Results of surgical treatment in supraventricular tachycardias]. (1993)

From May 1989 to May 1992, 44 patients (mean age 41 years, range 15-66) underwent surgery for supraventricular tachycardias: in 35 patients with atrioventricular reentrant tachycardia or atrial fibrillation associated with accessory pathway and refractory to medical treatment, the epicardial approach was used; in 8 with atrioventricular nodal reentrant tachycardia, a perinodal cryosurgery of the atrioventricular node was used, and in 1 patient with atrial flutter a cryosurgical ablation around the orifice of the coronary sinus and surrounding tissues was performed. All 38 accessory pathways were successfully ablated in 35 patients and no recurrences of delta wave or tachycardia were observed during a mean follow-up of 17 +/- 10 months. Atrial perforation during surgery and pericarditis were the only complications observed. All 8 patients with atrioventricular nodal reentrant tachycardia were successfully treated: in 2 patients dual pathways persisted after surgery but tachycardia was no longer inducible. No recurrences were observed during a mean follow-up of 15 +/- 4 months. Since surgery (15 months), the patient with atrial flutter has been free of recurrent episodes of atrial flutter. In conclusion, surgical treatment of supraventricular tachycardias is highly successful, with no mortality and very low morbidity. Should transcatheter ablation fail, surgery should be the treatment of choice in patients with frequent and symptomatic supraventricular tachycardias.
Structure and Function of a Human TAF <sub>II</sub> 250 Double Bromodomain Module (2000)

TFIID is a large multiprotein complex that initiates assembly of the transcription machinery. It is unclear how TFIID recognizes promoters in vivo when templates are nucleosome-bound. Here, it is shown that TAFII250, the largest subunit of TFIID, contains two tandem bromodomain modules that bind selectively to multiply acetylated histone H4 peptides. The 2.1 angstrom crystal structure of the double bromodomain reveals two side-by-side, four-helix bundles with a highly polarized surface charge distribution. Each bundle contains an Nepsilon-acetyllysine binding pocket at its center, which results in a structure ideally suited for recognition of diacetylated histone H4 tails. Thus, TFIID may be targeted to specific chromatin-bound promoters and may play a role in chromatin recognition.
A highly parallel signal processor (2002)

There is an increasing need for signal processors functional across a broad range of problems, from radar systems to E-O and ESM applications. To meet this challenge, a signal processing system capable of efficiently meeting the processing requirements over a broad range of avionics sensor systems has been developed. The CDC parallel modular signal processor (PMSP) is a complete MIL/E-5400-qualified digital signal processing system capable of computation rates greater than 600 MOPS (million operations per second). The signal processing element of the PMSP is the Micro-AFP. It is an all-VLSI processor capable of executing multiple simultaneous operations. Up to five Micro-AFPs and 12 MB of main store memory (MSM), along with associated control and I/O functions, are contained in the PMSP's standard ATR enclosure.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A GESTÃO DEMOCRÁTICA DA EDUCAÇÃO NO CONTEXTO DO MUNDO DO TRABALHO E DA REFORMA DO ESTADO (2017)

RESUMO: A partir dos anos 90 aparece uma nova estruturação do modo de produção capitalista, provocando consequentemente mudanças nas políticas econômicas, públicas, sociais e educacionais. A gestão da educação básica, no Brasil, forma parte desta mudança e está imersa na reforma do Estado, com dois grandes desafios: ajustar-se à configuração do novo sistema econômico, global, orientado pelos princípios do neoliberalismo e adaptar-se às necessidades da nova estrutura produtiva e tecnológica que exige novas perspectivas na formação dos trabalhadores. Diante desse contexto, este artigo tem como objetivo fazer uma reflexão de como as transformações no mundo do trabalho provocaram reformas no Estado brasileiro, assim como mudanças na gestão democrática da educação. Comprovando que a descentralização da gestão educativa na realidade é uma transferência das responsabilidades do Estado à Sociedade, reduzindo suas obrigações e seus gastos públicos. Não se consolidando, assim, um verdadeiro projeto democrático para a educação.
Run-time monitoring of architecturally significant behaviors using behavioral profiles and aspects (2006)

Although static structures are often advocated as the main ingredient of a software architecture, dynamic program behavior forms an essential part of it. Verifying the behavior is a crucial yet often troublesome part of testing. Hence, it is of great concern to find means to facilitate the testing of dynamic behaviors. This paper studies one approach to such behavioral monitoring. The details of the approach are the following. We have used the concept of behavioral profiles to specify the desired program behavior using UML. Provided with a behavioral profile created with a CASE tool, we are able to automatically generate AspectJ aspects that are woven into Java program code, thus adding a monitoring concern to the system. This results in the opportunity to monitor architecturally significant behaviors defined with architectural profiles at code level. Towards the end of the paper, we study the applicability of the approach in industrial use.
Epidemiology of meniscal lesions in the knee: 1,215 open operations in copenhagen 1982-84 (1990)

In a suburban area of Copenhagen with approximately 620,000 inhabitants, all the openly operated on meniscal lesions of the knee joint between 1982 and 1984 inclusive were reviewed. The mean annual incidence of meniscal lesions per 10,000 inhabitants was 9.0 in males and 4.2 in females. The highest incidences were seen in the 3rd, 4th, and 5th decades of life. A higher frequency of trauma related to onset of symptoms was found among males (77 percent) than among females (64 percent). The bucket-handle lesion was the most frequent type of meniscal lesion in males (35 percent), whereas peripheral detachment was the most frequent in females (41 percent). A variable frequency of trauma inducing the lesions in males and females did not explain differences in type of meniscal lesion. Nineteen percent of our 1,215 patients had a partial meniscectomy, whereas 0.7 percent had meniscal repair.