gebhart/patrepeval huggingface dataset:
Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice (2018)

PMX53 and PMX205 are cyclic hexapeptide inhibitors of complement C5a receptors (C5aR1), that are widely used to study C5aR1 pathobiology in mouse models of disease. Despite their widespread use, limited information regarding their pharmacokinetics have been reported. Here, a bioanalytical method for the quantitative determination of PMX53 and PMX205 in plasma, brain and spinal cord of mice was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques. The LC-MS/MS method was validated in all three matrices according to regulatory guidelines and successfully applied to pharmacokinetic studies of PMX53 and PMX205 in C57BL/6 J mice following intravenous administration. The developed method was highly sensitive and sufficiently accurate with a lower limit of quantification within the range of 3-6 ng/ml in extracted plasma samples and 3-6 ng/g in processed tissue samples, which outperforms previously published LC-MS/MS methods. The results thus support the suitability, reliability, reproducibility and sensitivity of this validated technique. This method can therefore be applied to perform a complete pre-clinical investigation of PMX53 and PMX205 pharmacokinetics in mice.
An amphiphilic pyrene sheet for selective functionalization of graphene (2011)

We have demonstrated that the aromatic amphiphile consisting of a hydrophilic dendron and an aromatic segment with a planar conformation can selectively exfoliate graphite powder into single- and double-layer graphene sheets in aqueous solution through hydrophilic functionalization of graphene surfaces.
On the application of link adaptation concepts to GSM (2002)

Link adaptation algorithms have been developed for 3rd generation systems to select the optimum combination of speech and channel coding for a particular link quality. This paper considers the application of the concept to GSM by a combining a new channel coding scheme with a GSM half rate voice coder on a full rate GSM channel. Gross rate link adaptation is achieved by switching between half rate and full rate channels when channel quality deteriorates; This paper demonstrates that a significant enhancement in coverage and perceived quality is possible with this procedure.
Oxidation of Cyclohexane with Molecular Oxygen Efficiently Catalyzed by Di-Iron(III)-Substituted Silicotungstate, γ-SiW10{Fe(OH2)}2O386−, Including Radical-chain Mechanism (1998)

Abstract The tetrabutylammonium salt of γ-SiW10{Fe(OH2)}2O386− showed high turnover number of 135-147 for the oxidation of cyclohexane with 1 atm molecular oxygen and the value is of the highest level among silicotungstates with various iron contents and other catalysts reported. Results obtained with radical initiator and scavenger, and the selectivity for oxygenation of adamantane suggest that the reaction involves a radical-chain path.
Inclusion of high molecular weight dextran in calcium phosphate-mediated transfection significantly improves gene transfer efficiency. (2007)

Calcium phosphate-based mammalian cell transfection is a widely used gene transfer technology. To facilitate the efficiency of this gene transfer method, several polysaccharide compounds were tested and evaluated for their effectiveness in enhancing DNA transfection. Using a HIV-1-derived lentivirus vector plasmid as a gene transfer indicator, we demonstrated that the addition of high molecular weight dextran-500 at 0.6-1.2% in the 2x Hepes buffered saline (HBS) increased transfection efficiency by over 50% (as reflected by the number of GFP-positive cells) and increased the titer of resulting lentivirus vector particles even more (up to 4-fold). This enhancement of transfection efficiency was further increased when higher molecular weight dextran formulations were used in place of dextran-500, and also when dextran was used in combination with polybrene, another polycationic chemical compound. Examination of transfected cells showed that dextran had no apparent adverse effect on cell viability and growth. Our data represent the first report showing that dextran can be used to enhance calcium phosphate-mediated gene transfer; this may be useful in applications for the generation of high-titer virus vector stocks using transient transfection technology.
Cyclohexylether -Amino Acids: New Leads for Selectivity Filters in Ion Channels (2001)

Synthetic ion channels containing δ-amino acids can become cation selective! δ-Amino acids with a cyclohexylether unit (see structure) were combined with structural motives from gramicidin A and led to channels with a NH4+/K+ permeability ratio of >10/1. (The current trace for an NH4+ channel is shown.)
A 50 to 70 GHz Power Amplifier Using 90 nm CMOS Technology (2009)

A 50 to 70 GHz wideband power amplifier (PA) is developed in MS/RF 90 nm 1P9M CMOS process. This PA achieves a measured P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">sat</sub> of 13.8 dBm, P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">dB</sub> of 10.3 dBm, power added efficiency (PAE) of 12.6%, and linear power gain of 30 dB at 60 GHz under V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DD</sub> biased at 1.8 V. When V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DD</sub> is biased at 3 V, it exhibits P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">sat</sub> of 18 dBm, P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">1</sub> <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">dB</sub> of 12 dBm, PAE of 15%, and linear gain of 32.4 dB at 60 GHz. The MMIC PA also has a wide 3 dB bandwidth from 50 to 70 GHz, with a chip size of 0.66 times 0.5 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . To the author's knowledge, this PA demonstrates the highest output power, with the highest gain among the reported CMOS PAs in V-band.
Previous mammograms in patients with impalpable breast carcinoma: retrospective vs blinded interpretation. 1993 ARRS President's Award. (1993)

Our results show that impalpable breast carcinomas are frequently evident in retrospect on previous mammograms. However, because many are manifested only as an asymmetric density, these may not necessarily be true radiologic errors. Failure to detect a retrospectively visible abnormality on a screening mammogram is not necessarily negligent, and retrospective reviews do not reflect the everyday practice of screening mammography.
Impact of periodontitis on the diabetes-related inflammatory status. (2010)

Wide-ranging activation of the innate immune system causing chronic low-grade inflammation is closely involved not only in the pathogenesis of type 2 diabetes mellitus and its complications, through an ongoing cytokine-induced acute-phase response, but also in the pathogenesis of periodontal diseases, whereby cytokines play a central role in the host's response to the periodontal biofilm. Although there is extensive knowledge about the pathways through which diabetes affects periodontal status, less is known about the impact of periodontal diseases on the diabetes-related inflammatory state. This review attempts to explain the immunobiological connection between periodontal diseases and type 2 diabetes mellitus, exploring the mechanisms through which periodontal infection can contribute to the low-grade general inflammation associated with diabetes (thus aggravating insulin resistance) and discussing the impact of periodontal treatment on glycemic control in people living with both diabetes and periodontal disease.
Three examples of <i>S</i> -integral points of elliptic curves over Q (1997)

article Free Access Share on Three examples of S-integral points of elliptic curves over Q Author: Horst G. Zimmer View Profile Authors Info & Claims ACM SIGSAM BulletinVolume 31Issue 2June 1997 pp 10–21https://doi.org/10.1145/261320.261326Online:01 June 1997Publication History 0citation116DownloadsMetricsTotal Citations0Total Downloads116Last 12 Months0Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Dynamics of Polymer Molecules in Dilute Solution: Viscoelasticity, Flow Birefringence and Dielectric Loss (1956)

The problem of the motions of a chain molecule diffusing in a viscous fluid under the influence of external forces or currents is considered for a particular model. This model is a chain of beads connected by ideal springs. Hydrodynamic interaction between the beads is introduced in the approximate form due to Kirkwood and Riseman. It is possible to solve this problem exactly with the use of a transformation to a set of normal coordinates. The viscosity, birefringence of flow, and dielectric and tensile relaxation behavior are calculated explicitly. The intrinsic viscosity in steady flow is somewhat different from the Kirkwood-Riseman result, and there is no change of viscosity with shear rate. The spectrum of relaxation times is similar to that found by Rouse and by F. Bueche, but has its maximum at a lower frequency than those obtained by Kuhn and Kuhn and by Kirkwood and Fuoss in other ways.
Determination of Iron‐Binding Phenolic Groups in Foods (1991)

ABSTRACT A spectrophotometric assay was developed with the specific aim of determining the content in foods of iron‐binding phenolic compounds. Foods were extracted with dimethylformamide (50%) and an ironcontaining reagent was added. The resulting color was due to formation of Fe‐galloyl and Fe‐catechol complexes. These complexes had different absorbance maxima and could thus be separately determined by readings at two wavelengths. The method was simple and had good precision, accuracy and analytical recovery. The new method was compared with two commonly used methods for determination of phenolic content in cereals, fruits, vegetables, spices and beverages.
Angled cavity broad area quantum cascade lasers (2012)

Angled cavity broad area quantum cascade lasers (QCLs) are investigated with surface grating-based distributed feedback (DFB) mechanisms. It is found that an angled cavity incorporating a one dimensional DFB with grating lines parallel to the laser facet offers the simplest solution for single mode and diffraction limited emission in the facet normal direction. A room temperature single mode QCL with the highest output power for wavelengths longer than 10 μm is demonstrated. This structure could be applied to a wide range of laser structures for power scaling along with spectral and spatial beam control.
Highly Phosphonated Polypentafluorostyrene (2011)

The synthesis and conductivity of highly phosphonated polypentafluorostyrene are demonstrated. Efficient postphosphonation (90%) was achieved via the classical nucleophilic aromatic substitution (SNAr) Michaelis–Arbuzov reaction of polypentafluorostyrene (PFS) with tris(trimethylsilyl) phosphite. The contrivance is in the cumulative electron-withdrawing effect of the fluorine functions. This simultaneously facilitates the SNAr reaction and enhances acidity of the resulting phosphonic acid. The most important consequence is a substantial increase of the H+ conductivity being the highest one measured on phosphonated polymer: σ = 0.1 S cm–1 at 108 °C, p = 105 Pa water vapor pressure. This value is 4 times higher than the one for poly(vinylphosphonic acid) (σ = 0.025 S cm–1) and higher than Nafion 117 (σ = 0.075 S cm–1) under the same conditions. Additionally, this polymer showed outstanding resistance to oxidative and thermal treatment (Tdecomp = 340 °C at 70% O2 atmosphere). All this makes the phosphonated PFS a very promising candidate as polymer electrolyte for fuel cell applications.
Evaluation of CD46 re-targeted adenoviral vectors for clinical ovarian cancer intraperitoneal therapy (2016)

Ovarian cancer accounts for >140 000 deaths globally each year. Typically, disease is asymptomatic until an advanced, incurable stage. Although response to cytotoxic chemotherapy is frequently observed, resistance to conventional platinum-based therapies develop rapidly. Improved treatments are therefore urgently required. Virotherapy offers great potential for ovarian cancer, where the application of local, intraperitoneal delivery circumvents some of the limitations of intravenous strategies. To develop effective, adenovirus (Ad)-based platforms for ovarian cancer, we profiled the fluid and cellular components of patient ascites for factors known to influence adenoviral transduction. Levels of factor X (FX) and neutralizing antibodies (nAbs) in ascitic fluid were quantified and tumor cells were assessed for the expression of coxsackie virus and adenovirus receptor (CAR) and CD46. We show that clinical ascites contains significant levels of FX but consistently high CD46 expression. We therefore evaluated in vitro the relative transduction of epithelial ovarian cancers (EOCs) by Ad5 (via CAR) and Ad5 pseudotyped with the fiber of Ad35 (Ad5T*F35++) via CD46. Ad5T*F35++ achieved significantly increased transduction in comparison to Ad5 (P<0.001), independent of FX and nAb levels. We therefore propose selective transduction of CD46 over-expressing EOCs using re-targeted, Ad35-pseudotyped Ad vectors may represent a promising virotherapy for ovarian cancer.
Fructose bisphosphatase of Escherichia coli: cloning of the structural gene (fbp) and preparation of a chromosomal deletion (1984)

The fbp locus at 96 min on the Escherichia coli chromosome governs fructose bisphosphatase (fructose-1,6-P2 1-phosphatase). We have cloned and subcloned fbp on vector pBR322 to obtain strains with high levels of the enzyme. In vivo mutagenesis of the clone was used to show that fbp is the structural gene. The gene was deleted on the plasmid in vitro, and the chromosomal wild-type locus was replaced with this deletion by a method involving stabilization of a heterozygous intermediate resulting from plasmid integration, followed by segregation of the wild-type gene.
Parametric excitation of bulk acoustic wave composite resonators (2002)

The theory of parametric excitation of acoustic waves in a bulk acoustic composite resonator has been developed. The mechanism of nonlinearity such as AC controlled boundary conditions is discussed. Numerical calculations revealed the possibility of parametric amplification of signals in the structures involved.
Enhancing Java server availability with JAS (2001)

Abstract The Java programming language is increasingly used in the implementation of servers with stringent availability, reliability, and performance requirements. Our Java Application Supervisor (JAS) software system is an attachment to a Java runtime environment that enhances the availability of a target Java program. To this end, JAS automatically detects and resolves certain reliability and performance problems during the execution of the target program. JAS does not require any source or byte code modifications in the target program. Instead, JAS is configured for a target program by supplying simple policies that determine how JAS reacts to problems during the target program execution. JAS typically imposes little execution time and memory overhead on the target program. We describe an experiment with a Web proxy that exhibits reliability and performance problems under heavy load. In this experiment, running the proxy in conjunction with JAS increased the rate of successful requests to the proxy by 33% and decreased the averagerequest processing time by 22%. JAS was also used successfully in two Java servers at Bell Labs to monitor server reliability and performance and ensure long‐term availability. Copyright © 2001 John Wiley & Sons, Ltd.
New Trends in Nucleic Acids Based Biosensors—Florence, Italy, October 25–28, 2003 (2004)

Abstract This review reports on the main arguments discussed during the workshop on “new trends in nucleic acids based biosensors” which was held at the University of Florence (Italy) from 25th to 28th October 2003. The review has been divided according to the three sessions of the workshop and it examines the state of art and the future trends in the field of nucleic acid‐based biosensors with particular attention on the aptamer field.
In Vitro Kinetics of Drug Release and Pulmonary Retention of Microencapsulated Antibiotic in Liposomal Formulations in Relation to the Lipid Composition (1997)

In previous in-vivo studies, we demonstrated that liposomal entrapment of tobramycin resulted in an increased availability of the antibiotic in the lungs without increasing bactericidal efficacy (Omri et al. 1994). With the aim of developing liposomal formulations allowing more efficient liposome-bacteria interactions, we studied the influence of lipid composition on both drug release and pulmonary retention of encapsulated tobramycin. The phase transition temperatures of nine liposome-tobramycin formulations consisting of two synthetic phospholipids (distearoyl phosphatidylcholine (DPSC) or dipalmitoyl phosphatidylcholine (DPPC) with dimyristoyl phosphatidyl-glycerol (DPMG) or dimyristoyl phosphatidylcholine (DMPC) were determined by differential scanning calorimetry. Liposomes, varying in terms of membrane fluidity and charge were submitted to in-vitro and in-vivo kinetic studies while retention and release of tobramycin were measured by high-performance liquid chromatography (HPLC). Five less fluid liposome formulations showed absence or very low tobramycin release in in-vitro tests and long term pulmonary retention of tobramycin. Four fluid liposome formulations showed in vitro tests modulated tobramycin release while pulmonary retention of tobramycin was dependent of the presence of charged phospholipids. Administration of charged fluid liposomes in mice showed a low level of tobramycin in the kidneys; non-charged fluid liposomes exhibited a relatively high level of tobramycin retention in the kidneys.
DESIGN OF PRODUCT PLACEMENT LAYOUT IN RETAIL SHOP USING MARKET BASKET ANALYSIS (2010)

Retailing is an industry with high level of competition. It is a customer-based industry which depends on how it could be aware of what the customers’ needs and requirements are. One technique most used in supermarkets is the mix merchandise. The purpose of this paper is to identify associated products, which then grouped in mix merchandise with the use of market basket analysis. This association between products then will be applied in the design layout of the product in the supermarket. The process of identifying the related products bought together in one transaction is done by using data mining technique. Apriori algorithm is chosen as a method in the data mining process. Using WEKA (Waikato Environment for Knowledge Analysis) software, the association rule between products is calculated. The results found five category association rules and fourteen sub-category association rules. These associations then will be interpreted as confidence and support to become consideration for the product layout.
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase (2011)

Acetate kinase, a member of the acetate and sugar kinase-Hsp70-actin (ASKHA) enzyme superfamily, is responsible for the reversible phosphorylation of acetate to acetyl phosphate utilizing ATP as a substrate. Acetate kinases are ubiquitous in the Bacteria, found in one genus of Archaea, and are also present in microbes of the Eukarya. The most well characterized acetate kinase is that from the methane-producing archaeon Methanosarcina thermophila. An acetate kinase which can only utilize PP(i) but not ATP in the acetyl phosphate-forming direction has been isolated from Entamoeba histolytica, the causative agent of amoebic dysentery, and has thus far only been found in this genus. In the direction of acetyl phosphate formation, acetate kinase activity is typically measured using the hydroxamate assay, first described by Lipmann, a coupled assay in which conversion of ATP to ADP is coupled to oxidation of NADH to NAD(+) by the enzymes pyruvate kinase and lactate dehydrogenase, or an assay measuring release of inorganic phosphate after reaction of the acetyl phosphate product with hydroxylamine. Activity in the opposite, acetate-forming direction is measured by coupling ATP formation from ADP to the reduction of NADP(+) to NADPH by the enzymes hexokinase and glucose 6-phosphate dehydrogenase. Here we describe a method for the detection of acetate kinase activity in the direction of acetate formation that does not require coupling enzymes, but is instead based on direct determination of acetyl phosphate consumption. After the enzymatic reaction, remaining acetyl phosphate is converted to a ferric hydroxamate complex that can be measured spectrophotometrically, as for the hydroxamate assay. Thus, unlike the standard coupled assay for this direction that is dependent on the production of ATP from ADP, this direct assay can be used for acetate kinases that produce ATP or PP(i).
Generation of High-Affinity Chicken Single-Chain Fv Antibody Fragments for Measurement of the<i>Pseudonitzschia pungens</i>Toxin Domoic Acid (2006)

Antibody-based assay systems are now accepted by regulatory authorities for detection of the toxins produced by phytoplankton that accumulate in shellfish tissues. However, the generation of suitable antibodies for sensitive assay development remains a major challenge. We have examined the potential of using the chicken immune system to generate high-affinity, high-specificity recombinant antibody fragments against phytotoxins. Following immunization of the chicken with domoic acid-bovine serum albumin, a single-chain antibody variable region (scFv) gene library was generated from single V(H) and V(L) genes isolated from the immune cells in the spleen and bone marrow. scFvs reacting with domoic acid were isolated by phage display and affinity matured by light chain shuffling, resulting in an approximate 10-fold increase in sensitivity. The isolated scFvs were effectively expressed in Escherichia coli and readily purified by affinity chromatography. They were then used to develop a convenient and sensitive indirect competitive enzyme-linked immunosorbent assay for domoic acid, with a 50% effective dose of 156 ng/ml, which could be used reliably with shellfish extracts. This study demonstrates that chickens provide a valuable model system for the simplified, rapid generation of high-affinity recombinant antibody fragments with specificity for small toxin molecules.
RING NMR dynamics: software for analysis of multiple NMR relaxation experiments (2020)

Molecular motions are fundamental to the existence of life, and NMR spectroscopy remains one of the most useful and powerful methods to measure their rates and molecular characteristics. Multiple experimental methods are available for measuring the NMR relaxation properties and these can require different methods for extracting model parameters. We present here a new software application, RING NMR Dynamics, that is designed to support analysis of multiple relaxation types. The initial release of RING NMR Dynamics supports the analysis of exponential decay experiments such as T<sub>1</sub> and T<sub>2</sub>, as well as CEST and R<sub>2</sub> and R<sub>1ρ</sub> relaxation dispersion. The software runs on multiple operating systems in both a command line mode and a user-friendly GUI that allows visualizing and simulating relaxation data. Interaction with another program, NMRFx Analyst, allows drilling down from the derived relaxation parameters to the raw spectral data.