gebhart/patrepeval huggingface dataset:
Introns are necessary for mRNA accumulation in <i>Schizophyllum commune</i> (1999)

The cDNA coding sequence of the Agaricus bisporus hydrophobin gene ABH1 under the regulation sequences of the Schizophyllum commune SC3 hydrophobin gene gave no expression in S. commune. In contrast, the genomic coding sequence (containing three introns) produced high levels of ABH1 mRNA when transformed to S. commune in the same configuration. Apparently, introns were needed for the accumulation of mRNAs from the ABH1 gene. When the effect of intron deletion on expression of the homologous genes SC3 and SC6 was examined, it was observed that only the genomic coding sequences were expressed in S. commune. Run-on analysis with nuclei harbouring intron-containing and intronless SC6 showed that this effect did not occur at the level of transcription initiation: genomic and cDNA sequences were equally active in this respect. When a 50 bp artificial intron containing the consensus splice and branch sites of S. commune introns, in addition to random-generated sequences, was introduced in the right orientation into the intronless SC3 transcriptional unit, accumulation of SC3 mRNA was restored. By polymerase chain reaction amplification, no unspliced SC3 mRNA species could be detected. Furthermore, the addition of an intron into the transcriptional unit of the gene for green fluorescent protein (GFP) effected clear fluorescence of the transgenic hyphae. Apparently, splicing is required for the normal processing of primary transcripts in S. commune.
Introduction to Programming with Java 3D (1998)

Java 3D is a new cross-platform API for developing 3D graphics applications in Java. Java 3D’s feature set has been designed to enable quick development of complex 3D applications, and at the same time enable fast and efficient implementations on a variety of platforms, from PCs to workstations. Using Java 3D, software developers can build cross-platform applications that build 3D scenes programmatically, or via loading 3D content from VRML, OBJ, and/or other external files. The Java 3D API includes a rich feature set for building shapes, composing behaviors, interacting with the user, and controlling rendering details. Participants in this tutorial will learn the concepts behind Java 3D, the Java 3D class hierarchy, typical usage patterns, ways of avoiding common mistakes, animation and scene design techniques, and tricks for increasing performance and realism.
Image annotation with tagprop on the MIRFLICKR set (2010)

Image annotation is an important computer vision problem where the goal is to determine the relevance of annotation terms for images. Image annotation has two main applications: (i) proposing a list of relevant terms to users that want to assign indexing terms to images, and (ii) supporting keyword based search for images without indexing terms, using the relevance estimates to rank images.
An Economical Compass and Clinometer for Basic Geology Courses (1959)

The writers believe that actual practice in basic field mapping enables students to gain a better understanding of abstract spatial relations necessary for intelligent interpretation of topographic maps. They therefore introduce this practice in the laboratory of their first year course. Since it would not be feasible to equip each member of such a class with a standard compass and clinometer, a simple, inexpensive instrument was designed that has proved satisfactory in use. This paper gives specifications and directions for its construction.
Eighth ACIS International Conference on Software Engineering, Artificial Intelligence, Networking, and Parallel/Distributed Computing-Cover (2007)

Given two machines A and B, A has to commit job codes to be executed on B and get the results back. How can A believe that the job codes are executed correctly on B and related privacy information is not revealed? We propose the attack model and the security goals during the remote execution process. To address this research problem, we propose a new method based on Trusted Computing and Virtualization. We extend trusted computing by providing property-based attestation, job keys generating and migrating securely in TPM. We extend virtualization by using TPM and MAC in hypervisor to isolate job VM. We examine the method to the security goals and it shows that this method can solve the problem effectively.
Use of an Autogenous Bacterin to Treat a Chronic Mouth Infection in a Reticulated Python (1974)

J. Bruce Addison, Elliott R. Jacobson, Use of an Autogenous Bacterin to Treat a Chronic Mouth Infection in a Reticulated Python, The Journal of Zoo Animal Medicine, Vol. 5, No. 1 (Mar., 1974), pp. 10-11
Restarting production in transition economies - political risk guarantee facilities (1998)

This note provides a look at the World Bank's commitment to re-activate the existing production facilities among its client countries, allowing these facilities to obtain the necessary inputs and financing for production. Therefore, the Political Risk Guarantee Facility (PGF) has been developed, to provide access to foreign capital, particularly for those projects with existing local enterprises. The note discusses on the practical aspects and mechanisms of the PGF operation and its uses elsewhere. It is in operation at present in Bosnia and Herzegovina, being expected to operate similarly for projects in Albania and Ukraine soon.
Spinnen auf Kopfsalatfeldern bei Kitzingen (Unterfranken, Bayern) (1999)

In den Jahren 1992 und 1993 wurden von L. Nunnenmacher zur Erfassung potentieller Blattlauspradatoren kurzzeitige Bodenfallentange aufVersuchs-f1achen mit Kopfsalatanbau durchgefOhrt. Die dabei erfaP..ten Spinnen wurden 1997 determiniert (Belege in coil. Blick). Die Artenliste und die Fangsummen sind bereits bei NUNNENMACHER (1998) genannt. Die Oaten sollen hiermit arachnologisch zuganglich gemacht werden.
Silver Nanoplates with Special Shapes:  Controlled Synthesis and Their Surface Plasmon Resonance and Surface-Enhanced Raman Scattering Properties (2006)

Shape-controlled synthesis of metal nanostructures has opened many new possibilities to design ideal building blocks for future nanodevices. In this work, new types of monodisperse silver nanoplates with complex shapes, namely, a disklike shape and flowerlike shapes, were controllably synthesized in high yield by reducing [Ag(NH3)2]+ with ascorbic acid in the presence of silver seed at room temperature. Unlike previous methods for synthesizing the silver nanoplates in the presence of cetyltrimethylammonium bromide (CTAB) micelles, the use of the precursor [Ag(NH3)2]+, other than Ag+, provides a flexible strategy to control the procession of the reduction reaction in a mild way. These silver nanoplates with shapes of disk and flower were shown to possess surface plasmon resonance (SPR) that directly relates to their geometric shapes. As a result of their high anisotropy in shape, the flowerlike silver nanoplates exhibit excellent surface-enhanced Raman scattering (SERS) enhancement ability relative to spherical silver nanoparticles and the disklike silver nanoplates. We believe that with the efficient synthesis and excellent SERS enhancement ability, these novel flowerlike silver nanoplates may find potential applications for biological sensing and labeling systems.
STIR turbo SE MR imaging vs. coregistered FDG‐PET/CT: Quantitative and qualitative assessment of N‐stage in non‐small‐cell lung cancer patients (2007)

STIR turbo SE imaging is at least as valid as coregistered FDG-PET/CT for quantitative and qualitative assessment of the N-stage for NSCLC patients.
Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology (2013)

Bacterial artificial chromosomes (BACs) are recombinant DNA molecules designed for propagation of large and instable foreign DNA fragment in Escherichia coli. BACs are used in genetics of large DNA viruses such as herpes and baculoviruses for propagation and manipulation of complex genomic regions or even entire viral genomes in one piece. Viral genomes in BACs are ready for the advanced tools of E. coli genetics. These techniques based on homologous or site-specific recombination allow engineering of virtually any kind of genetic changes. In the recent years, BAC technology was also adapted to manipulation of adenovirus genomes and became an effective alternative to traditional genetic engineering of recombinant adenoviruses.
MR‐guided intravascular procedures: Real‐time parameter control and automated slice positioning with active tracking coils (2004)

With carefully designed active catheters, a dedicated user interface, and an optimized pulse sequence intravascular interventions can successfully be performed by a single operator from within the MR scanner room.
Atmospheric Chemistry of Dimethyl Carbonate:  Reaction with OH Radicals, UV Spectra of CH<sub>3</sub>OC(O)OCH<sub>2</sub> and CH<sub>3</sub>OC(O)OCH<sub>2</sub>O<sub>2</sub> Radicals, Reactions of CH<sub>3</sub>OC(O)OCH<sub>2</sub>O<sub>2</sub> with NO and NO<sub>2</sub>, and Fate of CH<sub>3</sub>OC(O)OCH<sub>2</sub>O Radicals (1997)

A flash photolysis−resonance fluorescence technique was used to study the rate constant for the reaction of OH radicals with dimethyl carbonate over the temperature range 252−370 K. The rate constant exhibited a weak temperature dependence, increasing at both low and high temperature from a minimum value of approximately 3.1 × 10-13 cm3 molecule-1 s-1 near room temperature. Pulse radiolysis/transient UV absorption techniques were used to study the ultraviolet absorption spectra and kinetics of CH3OC(O)OCH2 and CH3OC(O)CH2O2 radicals at 296 K. Absorption cross sections of CH3OC(O)OCH2 and CH3OC(O)OCH2O2 at 250 nm were (3.16 ± 0.34) × 10-18 and (3.04 ± 0.43) × 10-18 cm2 molecule-1, respectively. Rate constants measured for the self-reactions of CH3OC(O)OCH2 and CH3OC(O)OCH2O2 radicals and reactions of CH3OC(O)OCH2O2 radicals with NO and NO2 were (5.6 ± 1.1) × 10-11, (1.27 ± 0.21) × 10-11, (1.2 ± 0.2) × 10-11, and (1.2 ± 0.2) × 10-11 cm3 molecule-1 s-1, respectively. The rate constant for reaction of F atoms with dimethyl carbonate was determined by a pulse radiolysis absolute rate technique to be (6.1 ± 0.9) × 10-11 cm3 molecule-1 s-1. A FTIR smog chamber system was used to show that, in 760 Torr of air at 296 K, CH3OC(O)OCH2O radicals are lost via three competing processes: 42 ± 15% via reaction with O2, 14 ± 2% via H atom elimination, and 44 ± 10% via decomposition and/or isomerization. Relative rate techniques were used to measure rate constants for the reactions of F atoms with CH3OC(O)OCH3, (6.4 ± 1.4) × 10-11 cm3 molecule-1 s-1, and Cl atoms with CH3OC(O)OCH3, CH3OC(O)OCH2Cl, CH3OC(O)OCHO, and HC(O)OC(O)OCHO, (2.3 ± 0.8) × 10-12, (4.6 ± 2.8) × 10-13, (1.7 ± 0.1) × 10-13, and (1.7 ± 0.1) × 10-14 cm3 molecule-1 s-1, respectively. Results are discussed in the context of the atmospheric chemistry of CH3OC(O)OCH3.
HPLC Method for Detection of Ergotamine, Ergosine, and Ergine after Intravenous Injection of a Single Dose (1996)

A simple, rapid method of liquid solid extraction coupled with a sensitive HPLC procedure with fluorescence detection to measure ergot alkaloid levels in bovine blood samples is presented. Single injections of either ergosine, ergotamine, or ergine were given to Holstein steer calves (140 kg), and serum concentrations of the ergot alkaloids were monitored over a 2-h period. Serum samples were loaded on a Bond Elut (100 mg) C18 column that had been washed with methanol and distilled water. Ergot alkaloids were then eluted with methanol and analyzed by HPLC. Recovery of ergosine, when added to serum at concentrations of 0.5−24.0 ng/mL, ranged from 95 to 98%. The data fit previous reports of three phases in elimination of drug. The method reported has excellent repeatability, a detection limit of 0.5 ng/mL, and provides an alternative to quantitate ergosine, ergotamine, and ergine in bovine blood samples at therapeutic dosages. Keywords: Ergosine; ergotamine; ergine; blood; assay; HPLC
Submillisecond response times of oxygen-quenched luminescent coatings (1993)

An apparatus and data analysis methodology is described which allows determination of response time to oxygen concentration changes of luminescent oxygen sensor coatings. Utilizing a solenoid valve, a sample chamber creates a pressure jump from 0.1 to 700 Torr in 600 μs that is followed by 15 ms of ringing. An optical detection system measures the response of porphyrin-based luminescent oxygen sensors to the pressure jump. The pressure in the chamber is measured simultaneously and independently with a piezoresistive pressure transducer. Data analysis techniques using nonlinear least-squares and numerical convolution of the luminescent response to the pressure rise allow determination of response times of the oxygen sensor. The response to pressure jumps of several luminescent oxygen sensitive coatings designed for video luminescent barometry are measured with this computer-controlled instrument. Several coatings were studied with response times of ∼2.5 s, ∼400 ms, 11 ms, 1.5 ms, and &lt;25 μs. Studies of the system suggest that we can determine response times down to about 10 μs.
In search of speculative thread-level parallelism (1999)

This paper focuses on the problem of how to find and effectively exploit speculative thread-level parallelism. Our studies show that speculating only on loops does not yield sufficient parallelism. We propose the use of speculative procedure execution as a means to increase the available parallelism. An additional technique, data value prediction, has the potential to greatly improve the performance of speculative execution. In particular, return value prediction improves the success of procedural speculation, and stride value prediction improves the success of loop speculation.
Thermal Durability of Wall-Flow Ceramic Diesel Particulate Filters (2001)

<div class="htmlview paragraph">The maximum use temperature of a diesel particulate filter is often thought to be limited only by the melting point of the filter material itself. This paper suggests that the maximum practical use temperature for filters is limited not by the intrinsic filter melting temperature, but by the temperature at which the metal oxide ash collected from the engine exhaust sinters and adheres to the filter wall, or the temperature at which the filter undergoes eutectic melting by reaction with the ash. Ash sintering and adherence without reaction with the filter material may result in loss of filter permeability and a permanent pressure drop increase. Chemical reactions between the ash and the filter can result either in pinholes through the walls, which compromise filtration efficiency, or glazing on the surface of the walls, which increases back pressure. These metal oxide deposits that comprise the ash are derived from engine wear, oil additives, fuel additives, and corrosion of the manifold and other exhaust system components.</div> <div class="htmlview paragraph">Excessive temperatures can be avoided by employing sophisticated regeneration controls and/or by employing filters that have a high volumetric heat capacity and/or a high thermal conductivity. Several new monolithic DPFs are under development that exhibit a unique combination of high thermal shock resistance and high volumetric heat capacity. Temperatures generated within these filters during uncontrolled regenerations are substantially lower than those observed for other ceramic oxide filters, resulting in little or no reaction between the filter and ash under severe operating conditions.</div>
Automated Assay for the Determination of Methylmalonic Acid, Total Homocysteine, and Related Amino Acids in Human Serum or Plasma by Means of Methylchloroformate Derivatization and Gas Chromatography–Mass Spectrometry (2005)

This assay combines a simple and automated sample preparation with selective and sensitive GC-MS analysis and is well suited for the combined measurement of MMA, tHcy, and the related amino acids.
Opposite Bone Remodeling Effects of Teriparatide and Alendronate in Increasing Bone Mass (2005)

Two distinct options for the management of osteoporosis lead to increases in BMD by opposite mechanisms of action on bone remodeling.
Membrane filtration of dairy products for microbiological analysis (1980)

Incubation with protease or Tween 80 or both dramatically improved the membrane filterability of liquid milks, powdered skim milk, and frozen dairy products without reducing the viability of five common species of bacteria. The technique can permit isolation and enumeration of microorganisms from test samples of these foods as large as 5 g. Flow direction through the filter was an important factor in filterability of dairy products.
Einleitung (2010)

Seit den ersten wissenschaftlichen Analysen von Materialflüssen in der Lieferkette von Forrester (1958) und Burbidge (1961) in den späten fünfziger und Anfang der sechziger Jahre sowie den ersten Erwähnungen des Begriffs „Supply Chain Management“ in den Arbeiten von Oliver und Webber (1982), Houlihan (1985) und Jones und Riley (1985) ist Supply Chain Management als Forschungsbereich der Wirtschaftswissenschaften von großer Bedeutung.
The Bioreactor With CYP3A4‐ and Glutamine Synthetase‐Introduced HepG2 Cells: Treatment of Hepatic Failure Dog With Diazepam Overdosage (2005)

A novel recombinant human hepatic cell line, CYP3A4- and glutamine synthetase (GS, an enzyme which converts ammonium ion and glutamate to glutamine)-introduced HepG2 (HepG2-GS-CYP3A4), was established. Its usefulness in a large-scale culture with a circulatory bioreactor in vitro and in dog models of ischemic hepatic failure with acute diazepam (DZP, a substrate of CYP3A4) overdosage was further examined. HepG2-GS-CYP3A4 expressed about 9 times larger amounts of CYP3A4 protein than a control. After incubation with HepG2-GS-3A4 cells in a circulatory bioreactor for 24 h, ammonia and DZP concentrations in the culture medium significantly decreased by about 40%. Furthermore, this system improved the survival time and decreased serum concentrations of DZP, ammonia, and transaminase in dogs with ischemic hepatic failure plus acute DZP overdosage. The mean survival time with bioreactor with HepG2-GS-3A4 was 42.7 +/- 3.6 h, which was significantly longer than that without reactor, with reactor (no cells), and with HepG2-GS (23.4 +/- 2.8, 22.1 +/- 2.4, and 31 +/- 3.7 h, respectively). Therefore, it is concluded that this bioartificial liver could be a good tool for the treatment of dogs with hepatic failure and that it could potentially be a bridging procedure to liver transplantation.
Soluble polyacetylene graft copolymers (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSoluble polyacetylene graft copolymersF. S. Bates and G. L. BakerCite this: Macromolecules 1983, 16, 4, 704–707Publication Date (Print):April 1, 1983Publication History Published online1 May 2002Published inissue 1 April 1983https://doi.org/10.1021/ma00238a040RIGHTS & PERMISSIONSArticle Views216Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alertsclose Get e-Alerts
Behavioural actions of neurohypophysial peptides (1980)

The neurohypophysial hormones vasopressin and oxytocin modulate memory processes. Vasopressin facilitates while oxytocin attenuates memory consolidation and retrieval. These influences are located in different regions of the molecules. Thus, the neurohypophysial hormones act as precursor molecules for neuropeptides involved in memory processes. The covalent ring structures of both vasopressin and oxytocin mainly affect consolidation, the linear parts, retrieval processes, while nearly the whole oxytocin or vasopressin molecule is needed for attenuation of consolidation and retrieval. Regional studies by microdissection techniques in combination with a sensitive radioenzymatic catecholalmine assay, indicate that vasopressin modulates memory processes by modulation of neurotransmission in distinct catecholamine systems. Recent experiments suggest that the influence of vasopressin on memory consolidation is mediated by the dorsal noradrenergic bundle via terminal regions of this bundle. Studies on the conversion of oxytocin in synaptosomal plasma membrane preparations of rat limbic brain suggest the possible generation of fragments with specific effects on memory processes. Regional differences in enzyme activity further substantiate the implication of oxytocin as a prohormone in this respect. Clinical studies support the evidence from laboratory findings that vasopressin is also involved in memory processes in man.
Enzymes in Organic Synthesis: Application to the Problems of Carbohydrate Recognition (Part 1) (1995)

Abstract Carbohydrates on cell surfaces are information molecules. Although only seven or eight monosaccharides are commonly used as building blocks in mammalian systems, the multifunctionality of these monomers can lead to the assembly of an immense variety of complex structures. Millions of different tetrasaccharide structures, for example, can be constructed from this small number of building blocks, if branching, the stereochemistry of glycosidic linkages, and the modification of hydroxyl and amino groups are taken into consideration. Oligosaccharides therefore represent an effective class of biomolecules that code for a vast amount of information required in various biological recognition processes, such as intercellular communication, signal transduction, cell adhesion, infection, cell differentiation, development and metastasis. The pace of development of pharmaceuticals based on carbohydrates has, however, been slower than that based on other classes of biomolecules. Part of the reason is the lack of technologies for the study of complex carbohydrates. There is no method to amplify oligosaccharides for sequence analysis. There is no machine available for automated synthesis of oligosaccharides. In addition, the possibly poor bioavailability and difficulties in the large‐scale synthesis of carbohydrates have undoubtedly contributed to this slow pace. The enzymatic and chemoenzymatic methods, especially those based on aldolases and glycosyltransferases, described here appear to be useful for the synthesis of mono‐ and oligosaccaharides and related molecules. Further advances in glycobiology will probably lead to the development of new technologies for the study of carbohydrate recognition and for the synthesis of bioactive carbohydrates and mimetics to control the recognition processes.