gebhart/patrepeval huggingface dataset:
Inhibition of Murine Leukemia Viruses by Nuclease-Resistant α-Oligonucleotides (1992)

We studied the antiviral activity of nuclease-resistant alpha-anomeric oligonucleotides. An alpha-oligonucleotide (20-mer) targeted to the primer binding site (PBS) of murine retroviruses inhibited viral spreading. The inhibition only occurred when the cells had been electropermeabilized in the presence of the oligonucleotide. The PBS sequence is involved in reverse transcription and in translation. The data suggest that the oligonucleotide could perturb reverse transcription activity. Thus, either the oligonucleotide induced a decrease in initiation or it inhibited the extension of the minus or plus strands DNA during reverse transcription. These results show that reverse transcription may be an interesting target for antisense oligonucleotides.
Protein Kinase C   Plays a Key Role in Cellular Senescence Programs of Human Normal Diploid Cells (2009)

In the present study, we clarified that transforming growth factor beta (TGF-beta) induces cellular senescence in human normal diploid cells, TIG-1, and identified protein kinase Cs (PKCs) as downstream mediators of TGF-beta-induced cellular senescence. Among PKCs, we showed that PKC-delta induced cellular senescence in TIG-1 cells and was activated in replicatively and prematurely senescent TIG-1 cells. The causative role of PKC-delta in cellular senescence programs was demonstrated using a kinase negative PKC-delta and small interfering RNA against PKC-delta. Furthermore, PKC-delta was shown to function in human telomerase reverse transcriptase (hTERT) gene repression. These results indicate that PKC-delta plays a key role in cellular senescence programs, and suggest that the induction of senescence and hTERT repression are coordinately regulated by PKC-delta.
Crystal structure of triaqua(1,10-phenanthroline-N,N’)sulfatonickel(II) hydrate, Ni(H2O)3(C12H8N2)(SO4) · H2O (2002)

Article Crystal structure of triaqua(1,10-phenanthroline-N,N’)sulfatonickel(II) hydrate, Ni(H2O)3(C12H8N2)(SO4) · H2O was published on December 1, 2002 in the journal Zeitschrift für Kristallographie - New Crystal Structures (volume 217, issue 1).
High-Selectivity Tunable Balanced Bandpass Filter With Constant Absolute Bandwidth (2016)

This brief presents a new and effective approach for designing a high-selectivity tunable balanced bandpass filter (BPF) with constant absolute bandwidth (ABW). The filter is composed of two open-loop resonators with a pair of symmetrically loaded varactor diodes to obtain tunable responses. The major design concept relies on choosing the particular position on the resonator for loading the varactor diodes. The desired variation slopes of the external quality factor and coupling coefficient can be easily obtained for realizing the constant ABW of the tunable BPF. Frequency-dependent source-load coupling between the feed lines are introduced for realizing two adaptive transmission zeros (TZs) on both sides of the tunable passband. Thus, during the frequency-tuning process, high selectivity of the proposed filter can be kept. Meanwhile, the common-mode suppression is optimized to an acceptable level in the frequency-agile range of the differential-mode passband by loading the stub and resistor in the series at the center of the resonator. For demonstration, a tunable balanced BPF with the constant ABW and two TZs is designed and implemented. The simulated and measured results are presented, showing good agreement.
Review of magnetocaloric effect in perovskite-type oxides (2013)

We survey the magnetocaloric effect in perovskite-type oxides (including doped ABO3-type manganese oxides, A3B2O7-type two-layered perovskite oxides, and A2B'B''O6-type ordered double-perovskite oxides). Magnetic entropy changes larger than those of gadolinium can be observed in polycrystalline La1−xCaxMnO3 and alkali-metal (Na or K) doped La0.8Ca0.2MnO3 perovskite-type manganese oxides. The large magnetic entropy change produced by an abrupt reduction of magnetization is attributed to the anomalous thermal expansion at the Curie temperature. Considerable magnetic entropy changes can also be observed in two-layered perovskites La1.6Ca1.4Mn2O7 and La2.5−xK0.5+xMn2O7+δ (0 < x < 0.5), and double-perovskite Ba2Fe1+XMo1−xO6 (0 ≤ x ≤ 0.3) near their respective Curie temperatures. Compared with rare earth metals and their alloys, the perovskite-type oxides are lower in cost, and they exhibit higher chemical stability and higher electrical resistivity, which together favor lower eddy-current heating. They are potential magnetic refrigerants at high temperatures, especially near room temperature.
The Fusome Mediates Intercellular Endoplasmic Reticulum Connectivity in<i>Drosophila</i>Ovarian Cysts (2004)

Drosophila ovarian cysts arise through a series of four synchronous incomplete mitotic divisions. After each round of mitosis, a membranous organelle, the fusome, grows along the cleavage furrow and the remnants of the mitotic spindle to connect all cystocytes in a cyst. The fusome is essential for the pattern and synchrony of the mitotic cyst divisions as well as oocyte differentiation. Using live cell imaging, green fluorescent protein-tagged proteins, and photobleaching techniques, we demonstrate that fusomal endomembranes are part of a single continuous endoplasmic reticulum (ER) that is shared by all cystocytes in dividing ovarian cysts. Membrane and lumenal proteins of the common ER freely and rapidly diffuse between cystocytes. The fusomal ER mediates intercellular ER connectivity by linking the cytoplasmic ER membranes of all cystocytes within a cyst. Before entry into meiosis and onset of oocyte differentiation (between region 1 and region 2A), ER continuity between cystocytes is lost. Furthermore, analyses of hts and Dhc64c mutants indicate that intercellular ER continuity within dividing ovarian cysts requires the fusome cytoskeletal component and suggest a possible role for the common ER in synchronizing mitotic cyst divisions.
Independent Intramolecular Mediators of Folding, Activity, and Inhibition for the Plasmodium falciparum Cysteine Protease Falcipain-2 (2004)

The Plasmodium falciparum cysteine protease falcipain-2 is a trophozoite hemoglobinase and potential antimalarial drug target. Unlike other studied papain family proteases, falcipain-2 does not require its prodomain for folding to active enzyme. Rather, folding is mediated by an amino-terminal extension of the mature protease. As in related enzymes, the prodomain is a potent inhibitor of falcipain-2. We now report further functional evaluation of the domains of falcipain-2 and related plasmodial proteases. The minimum requirement for folding of falcipain-2 and four related plasmodial cysteine proteases was inclusion of a 14-15-residue amino-terminal folding domain, beginning with a conserved Tyr. Chimeras of the falcipain-2 catalytic domain with extensions from six other plasmodial proteases folded normally and had kinetic parameters (k(cat)/K(m) 124,000-195,000 M(-1) s(-1)) similar to those of recombinant falcipain-2 (k(cat)/K(m) 120,000 M(-1) s(-1)), indicating that the folding domain is functionally conserved across the falcipain-2 subfamily. Correct folding also occurred when the catalytic domain was refolded with a separate prodomain-folding domain construct but not with an isolated folding domain peptide. Thus, the prodomain mediated interaction between the other two domains when they were not covalently bound. The prodomain-catalytic domain interaction was independent of the active site, because it was blocked by free inactive catalytic domain but not by the active site-binding peptide leupeptin. The folded catalytic domain retained activity after purification from the prodomain-folding domain construct (k(cat)/K(m) 168,000 M(-1) s(-1)), indicating that the folding domain is not required for activity once folding has been achieved. Activity was lost after nonreducing gelatin SDS-PAGE but not native gelatin PAGE, indicating that correct disulfide bonds are insufficient to direct appropriate folding. Our results identify unique features of the falcipain-2 subfamily with independent mediation of activity, folding, and inhibition.
Recombinant CD4- <i>Pseudomonas</i> Exotoxin Hybrid Protein Displays HIV-Specific Cytotoxicity without Affecting MHC Class II-Dependent Functions (1990)

The present study describes several in vitro activities of CD4(178)-PE40, a recombinant protein containing a portion of human CD4 linked to active regions of Pseudomonas aeruginosa exotoxin A. Using assays for cell viability, we demonstrate that the hybrid toxin displays highly selective cytotoxicity for HIV-infected T lymphocytes. In a latently infected human T-cell line which is inducible for HIV expression, toxin sensitivity is observed only upon virus induction. At concentrations which readily kill HIV-infected T cells, CD4(178)-PE40 has no observable cytotoxic effects on uninfected human cell lines expressing surface major histocompatibility complex (MHC) Class II molecules, and does not interfere with cellular responses known to be dependent on functional association between CD4 and MHC Class II molecules.
Developmental regulation of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionic acid receptor subunit expression in forebrain and relationship to regional susceptibility to hypoxic/ischemic injury. II. Human cerebral white matter and cortex (2006)

This is the first part of a two-part study to investigate the cellular distribution and temporal regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits in the developing white matter and cortex in rat (part I) and human (part II). Western blot and immunocytochemistry were used to evaluate the differential expression of AMPAR subunits on glial and neuronal subtypes during the first 3 postnatal weeks in the Long Evans and Sprague Dawley rat strains. In Long Evans rats during the first postnatal week, GluR2-lacking AMPARs were expressed predominantly on white matter cells, including radial glia, premyelinating oligodendrocytes, and subplate neurons, whereas, during the second postnatal week, these AMPARs were highly expressed on cortical neurons, coincident with decreased expression on white matter cells. Immunocytochemical analysis revealed that cell-specific developmental changes in AMPAR expression occurred 2-3 days earlier by chronological age in Sprague Dawley rats compared with Long Evans rats, despite overall similar temporal sequencing. In both white and gray matter, the periods of high GluR2 deficiency correspond to those of regional susceptibility to hypoxic/ischemic injury in each of the two rat strains, supporting prior studies suggesting a critical role for Ca2+-permeable AMPARs in excitotoxic cellular injury and epileptogenesis. The developmental regulation of these receptor subunits strongly suggests that Ca2+ influx through GluR2-lacking AMPARs may play an important role in neuronal and glial development and injury in the immature brain. Moreover, as demonstrated in part II, there are striking similarities between rat and human in the regional and temporal maturational regulation of neuronal and glial AMPAR expression.
A Comprehensive Survey of Blockchain: From Theory to IoT Applications and Beyond (2019)

As an innovated and revolutionized technology, blockchain has been applied in many fields, such as cryptocurrency, food traceability, identity management, or even market prediction. To discover its great potential, both industry and academia have paid great attention to it and numerous researches have been conducted. Based on the literature and industry whitepapers, in this survey, we unroll and structure the blockchain related discoveries and scientific results in many aspects. Particularly, we classify blockchain technologies into four layers and carry out a comprehensive study on the consensus strategies, the network, and the applications of blockchain. Different blockchain applications are put into the corresponding categories based on the fields, especially in Internet of Things (IoT). When introducing each layer, we not only organize and summarize the related works, but also discuss the fundamental issues and future research directions. We hope this survey could shed some light on the research of blockchain and serve as a guide for further studies.
Research note: concept and method in researching the football crowd (1997)

(1997). Research note: concept and method in researching the football crowd. Leisure Studies: Vol. 16, No. 2, pp. 127-131.
Decomposition of linoleic acid hydroperoxides. Enzymic reactions compared with nonenzymic (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDecomposition of linoleic acid hydroperoxides. Enzymic reactions compared with nonenzymicHarold W. GardnerCite this: J. Agric. Food Chem. 1975, 23, 2, 129–136Publication Date (Print):March 1, 1975Publication History Published online1 May 2002Published inissue 1 March 1975https://doi.org/10.1021/jf60198a012RIGHTS & PERMISSIONSArticle Views708Altmetric-Citations189LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alerts Get e-Alerts
Engineering sub-50 nm quantum effect devices and single-electron transistors using electron-beam lithography (1992)

Fabrication and characteristics of various nanoscale quantum and single-electron devices are described. Using high-resolution electron beam lithography and double layer PMMA resist, we have successfully fabricated a 20 nm split-gate device, a novel 50 nm single barrier single-electron transistor, and a 75 nm triple barrier tunneling device. Effects of beam dose and the number of writing passes on the actual lithography pattern were investigated for various device gate geometries. Pronounced quantum and single-electron effects have been observed in these devices.
Modelo de evaluación del potencial de desarrollo, investigación e innovación basado en software libre, en programas académicos de Ingeniería de Sistemas– El Framework PDII (2011)

espanolEste articulo describe un modelo para evaluar elpotencial de desarrollo, investigacion e innovacionde un programa academico basando dichopotencial, en la filosofia del Software Libre (SL).Esta propuesta se enfoca a programas academicosen ciencias de la computacion y se divide en tresareas de evaluacion para poder determinar los tresaspectos es decir, el desarrollo, la investigacion yla innovacion. La primer area de evaluacion es elescenario de aplicaciones computacionales queapoyan el trabajo de los docentes y administrativosdel programa academico, la segunda es elsegmento academico que determina el potencialde los docentes para innovar en sus actividadesacademicas segun su preparacion en software libre,finalmente se evalua el area de investigacion quedetermina la productividad en software libre delprograma academico. EnglishThis paper describes a model to evaluate the potentialof development, investigation and invention inan academic program basing this potential, in thefreeware philosophy. This proposal focuses on tocomputer sciences academic programs and himselfdivide in three areas of evaluation to determinethe three aspects that is development, investigationand invention. The first area of evaluation is thecomputational applications scene that support thework of both professors and staff of the academicprogram, the second the academic sector determinesthe potential of professors to innovate in theiracademic activities of his training in free software,finally evaluates the research area that determines theproductivity in freeware of the academic program.
The Very Important Partner program: integrating family and friends into the health care experience. (1999)

The hospital experience, especially in critical care units, can be perceived by patients and their family as unwelcoming, intimidating, and anxiety producing. Patients desire the presence of loved ones and family members and may commonly express feelings of helplessness and not feeling valued or important. The Very Important Partner (VIP) program integrates family and friends into the health care experience in order to create an environment that provides opportunities for patients and their loved ones to truly experience healing of the mind, body, and spirit. Patient satisfaction survey results and anecdotal comments demonstrate that the VIP program has a significantly positive effect on the perception of the overall hospital stay. The program is a simple, common sense approach to optimizing the overall healing of patients and family. The implementation of similar programs is recommended in hospitals nationwide.
Architecture-oriented visualization (1996)

Tracking the changing dynamics of object-oriented frameworks[5], design patterns[7], architectural styles[8], and subsystems during the development and reuse cycle can aid producing complex systems. Unfortunately, current object-oriented programming tools are relatively oblivious to the rich architectural abstractions in a system.This paper shows that architecture-oriented visualization, the graphical presentation of system statics and dynamics in terms of its architectural abstractions, is highly beneficial in designing complex systems. In addition, the paper presents architecture-aware instrumentation, a new technique for building efficient on-line instrumentation to support architectural queries. We demonstrate the effectiveness and performance of the scheme with case studies in the design of the Choices object-oriented operating system.
Stereospecificity in Metallocene Catalyzed Acrylate Polymerizations:  The Chiral Orientation of the Growing Chain Selects Its Own Chain End Enantioface (2006)

The mechanism of stereocontrol in the site-controlled stereospecific polymerization of methyl methacrylate (MMA) with C2- and CS-symmetric zirconocenes is investigated with a DFT based approach. Our model explains the experimentally observed isospecificity in MMA polymerization with the C2-symmetric rac-C2H4(Ind)2Zr-based system as well as the experimentally observed syndiospecificity in MMA polymerization with the CS-symmetric Me2C(Cp)(Flu)Zr-based system. In both cases, the chiral metallocene induces a chiral orientation of the ester enolate growing chain. In turn, the chirally oriented growing chain selects its own enantioface. Comparison with the mechanism of stereocontrol operative in the case of propene polymerization by the same zirconocenes is performed to stress similarities and differences. Although analogies are expected, surprising differences also exist. The most peculiar is that the in the case of 1-olefins, the enantioselective event is selection between the enantiofaces of the prochiral monomer. Instead, in the case of acrylates, the enantioselective event is selection between the enantiofaces of the prochiral growing chain.
Impedance probe to measure local void fraction profiles (1988)

A conductivity-type local void measurement system has been developed. The effects of the sensor tip geometry, the unbalance of the front-end bridge, the comparator threshold level, and the mass fluxes on the response of the instrument have been studied. The system has been calibrated under air–water two-phase flow conditions using the quick-closing-valve technique. Comparison of the void profiles obtained with the conductivity probe with those obtained using an optical probe confirms the applicability of this system for two-phase (air–water) flows.
In vivo generalized diffusion tensor imaging (GDTI) using higher‐order tensors (HOT) (2009)

Generalized diffusion tensor imaging (GDTI) using higher-order tensor (HOT) statistics generalizes the technique of diffusion tensor imaging by including the effect of nongaussian diffusion on the signal of MRI. In GDTI-HOT, the effect of nongaussian diffusion is characterized by higher-order tensor statistics (i.e., the cumulant tensors or the moment tensors), such as the covariance matrix (the second-order cumulant tensor), the skewness tensor (the third-order cumulant tensor), and the kurtosis tensor (the fourth-order cumulant tensor). Previously, Monte Carlo simulations have been applied to verify the validity of this technique in reconstructing complicated fiber structures. However, no in vivo implementation of GDTI-HOT has been reported. The primary goal of this study is to establish GDTI-HOT as a feasible in vivo technique for imaging nongaussian diffusion. We show that probability distribution function of the molecular diffusion process can be measured in vivo with GDTI-HOT and be visualized with three-dimensional glyphs. By comparing GDTI-HOT to fiber structures that are revealed by the highest resolution diffusion-weighted imaging (DWI) possible in vivo, we show that the GDTI-HOT can accurately predict multiple fiber orientations within one white matter voxel. Furthermore, through bootstrap analysis we demonstrate that in vivo measurement of HOT elements is reproducible, with a small statistical variation that is similar to that of diffusion tensor imaging.
Evaluation of Antibiotcis Used for Enterohemorrhagic Escherichia coli O157 Enteritis (1997)

We tested antimicrobial activities of ten oral antibiotics; ampicillin (ABPC), cefdinir (CFDN), cefaclor (CCL), fosfomycin (FOM), norfloxacin (NFLX), nalidixic acid (NA), kanamycin (KM), minocycline (MINO), doxycycline (DOXY), and tetracycline (TC) against eleven enterohemorrhagic Esherichia coli (EHEC) O157 clinical strains. Two strains were resistant to ABPC and TC. Other strains were sensitive to all the ten antibiotics. To investigate the effect of antibiotics on extracellular release of verotoxin (VT), strain EHEC TT10 was grown in 10 ml of LB containing various concentrations of the antibiotics for 2 h. Number of viable cells were counted and the amounts of VT1 and VT2 released in the supernatants were measured with reverse passive latex agglutination (RPLA) using serially diluted sterilized culture supernatants. The amount of VT1 and VT2 was evidently increased with ABPC, CFDN, CCL, and FOM, the inhibitors of cell wall biosynthesis. In the case of quinolons, VT2 was markedly increased, but VT1 was not released to the supernatant. KM killed the bacteria efficiently, but no release of VT1 or VT2 was observed in the supernatant. Tetracyclines (MINO, DOXY, and TC) did not make the bacteria release either VT1 or VT2, but could not kill the bacteria appreciably. These results indicated that the inhibitors of protein synthesis (KM, MINO, DOXY, TC) are the safe antibiotics not causing the release of verotoxin from the cells and thus preventing development of hemolytic uremic syndrome (HUS) or thrombotic thrombocytopenic purpra (TTP), the important sequelae of the enteritis.