gebhart/patrepeval huggingface dataset:
Production of gastric intrinsic factor, transcobalamin, and haptocorrin in opossum kidney cells (2000)

Opossum kidney epithelial cells were shown previously to synthesize and secrete two cobalamin (Cbl)-binding proteins, presumed to be haptocorrin (Hc) and transcobalamin II (TCII). The present study examines the hypothesis that renal tubular cells also produce intrinsic factor (IF), and this production provides an explanation for the presence of IF in urine. By using antisera raised against human IF and against TCII, the presence of TCII was confirmed, and that of IF discovered in the media of opossum kidney (OK) cells in culture. The apparent molecular weight of IF and TCII was 68 and 43 kDa, respectively. Immunoreactivity on Western blot of the putative IF protein was blocked by recombinant human IF. When proteins secreted into the media were separated electrophoretically under nondenaturing conditions after binding with [(57)Co]Cbl, a broad major band migrated at a relative front independently of recombinant IF or TCII, and probably represents Hc, as the Cbl binding is blocked by cobinamide. Small amounts of bound [(57)Co]Cbl migrated in the position of both IF and TCII, when cobinamide was present. The presence of IF and TCII in OK cells was confirmed by immunohistology. Specific reactivity for IF (blocked by recombinant IF) was found in proximal tubules of opossum kidney, but not in other portions of the nephron, confirming the ability of anti-human IF antiserum to detect opossum IF. A 732-bp fragment of IF, nearly identical in sequence to rat IF, was isolated by RT-PCR from opossum kidney mRNA, and Western blot confirmed the presence of IF protein. The presence of IF was also documented in rat kidney by isolation of an RT-PCR fragment, immunocytochemistry, and Western blot. IF should be added to the list of renal (proximal) tubular antigens that are shared by other epithelia.
Argonaute2 Regulates the Pancreatic β-Cell Secretome (2013)

Argonaute2 (Ago2) is an established component of the microRNA-induced silencing complex. Similar to miR-375 loss-of-function studies, inhibition of Ago2 in the pancreatic β-cell resulted in enhanced insulin release underlining the relationship between these two genes. Moreover, as the most abundant microRNA in pancreatic endocrine cells, miR-375 was also observed to be enriched in Ago2-associated complexes. Both Ago2 and miR-375 regulate the pancreatic β-cell secretome, and by using quantitative mass spectrometry, we identified the enhanced release of a set of proteins or secretion "signatures " in response to a glucose stimulus using the murine β-cell line MIN6. In addition, the loss of Ago2 resulted in the increased expression of miR-375 target genes, including gephyrin and ywhaz. These targets positively contribute to exocytosis indicating they may mediate the functional role of both miR-375 and Ago proteins in the pancreatic β-cell by influencing the secretory pathway. This study specifically addresses the role of Ago2 in the systemic release of proteins from β-cells and highlights the contribution of the microRNA pathway to the function of this cell type.
Yunantaxusin A, a New 11(15→1)-abeo-Taxane from Taxus yunnanensis (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTYunantaxusin A, a New 11(15→1)-abeo-Taxane from Taxus yunnanensisShunxiang Zhang, Catherine Tung-Ling Lee, Yoshiki Kashiwada, Ke Chen, De-Cheng Zhang, and Kuo-Hsiung LeeCite this: J. Nat. Prod. 1994, 57, 11, 1580–1583Publication Date (Print):November 1, 1994Publication History Published online1 July 2004Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/np50113a020https://doi.org/10.1021/np50113a020research-articleACS PublicationsRequest reuse permissionsArticle Views93Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Incidence of obstructive uropathy in male B6C3F1 mice on a 24-month carcinogenicity study and its apparent prevention by ochratoxin A. (1986)

A 24-month study assessed the carcinogenic potential of the nephrotoxic mycotoxin ochratoxin A (OA) in B6C3F1 mice. Three groups of 50 males and 50 females were fed 0.1 or 40 ppm OA in the diet. Obstructive urinary tract disease (mouse urological syndrome [MUS]) accounted for the greatest number of spontaneous deaths in the male mice of control (12/50) and 1 ppm (13/50) dose groups, but the disease was not observed in the males fed 40 ppm OA. The earliest age of onset of clinical signs of MUS was 4 months and the average age of onset was 10.1 months. The first death from MUS was observed at 5 months and average age at death was 12.2 months. The mice were caged in groups of five mice per cage and clustering of cases of MUS was observed. Properties of OA which may be important to its preventive effect include inhibition of growth of gram positive bacteria and the production of polyuria as a result of renal proximal tubular damage.
A Digital Circuit for Jitter Reduction of GPS-disciplined 1-pps Synchronization Signals (2007)

The Global Positioning System (GPS) satellites transfer accurate time from atomic clocks, thus enabling the receivers on Earth to produce high-stability synchronization signals (i.e., trains of low-jitter pulses without drift). The timing accuracy of the generated stream of pulses depends on the features as well as on the cost of the specific GPS receiver employed. This paper describes a fully digital synchronization circuit that is able to reduce the jitter associated to the 1 pulse per second (1-pps) signal generated by a typical low-cost receiver of moderate timing accuracy within a short settling time interval. The proposed circuit has been implemented using an FPGA and the jitter reduction has been estimated experimentally.
Support vector machine and parametric wavelet-based texture classification of stem cell images (2004)

Stem cell research is one of the most promising and cutting-edge fields in the medical sciences. It is believed that this innovative research will lead to lifesaving treatments in the coming years. As part of their work, stem cell researchers must first determine which of their stem cell colonies are of sufficiently high quality to be suitable for experimental studies and therapeutic treatments. Since colony texture is a major discriminating feature in determining quality, we introduce a non-invasive, semi-automated texture-based stem cell colony classification methodology to aid researchers in colony quality control. We first consider the general problem of textural image segmentation. In a new approach to this problem, we characterize image texture by the subband energies of the image's wavelet decomposition, and we employ a non-parametric support vector machine to perform the classification that yields the segmentation. We also adapt a parametric wavelet-based classifier that utilizes the Kullback-Leibler distance. We apply both methods to a set of benchmark textural images, report low segmentation error rates and comment on the applicability of and tradeoffs between the non-parametric and parametric segmentation methods. We then apply the two classifiers to the segmentation of stem cell colony images into regions of varying quality. This provides stem cell researchers with a rich set of descriptive graphical representations of their colonies to aid in quality control. From these graphical representations, we extract colony-wise textural features to which we add colony-wise border features. Taken together, these features characterize overall colony quality. Using these features as inputs to a multiclass support vector machine, we successfully categorize full stem cell colonies into several quality categories. This methodology provides stem cell researchers with a novel, non-invasive quantitative quality control tool.
Gastrointestinal transit of technetium-99m-labeled cellulose fiber and indium-111-labeled plastic particles. (1989)

We introduce two new nondigestible solid markers for gastrointestinal transit measurements. One is technetium-99m-labeled cellulose fiber [99mTc]CF, the other is indium-111-labeled plastic particles [111In]PP of 2- to 3-mm diameter. In six healthy male volunteers gastric emptying and small intestinal transit of the two markers were obtained simultaneously. Large intestinal transit of [111In]PP was also obtained. Technetium-99m CF had acceptable stability properties in the proximal gastrointestinal segments. Indium-111 PP was almost completely stable in all segments. Mean gastric emptying time was 1.13 +/- 0.24 hr (mean +/- s.d.) for [99mTc]CF and 1.94 +/- 0.78 hr for [111In]PP. The difference was significant (p less than 0.05). Mean small intestinal transit time was 3.85 +/- 0.61 hr (mean +/- s.d.) for [99mTc]CF and 4.03 +/- 0.34 hr for [111In]PP. The difference was not significant (p less than 0.5). Mean large intestinal transit time of [111In]PP was 23 +/- 11 hr (mean +/- s.d.). We also suggest a simple deconvolution principle for the interpretation of the small intestinal and the large intestinal transit data.
Leucite content of selected dental porcelains (1994)

Leucite is a major crystalline component of dental porcelains. The presence of tetragonal leucite in dental porcelains increases their coefficients of thermal expansion due to its high coefficient of thermal expansion (20-25 x 10(-6)/degrees C). This is particularly useful for those porcelains designed for bonding to precious metals and nickel alloys. The purpose of this study was to determine the leucite content of selected commercial dental porcelains in relation to their coefficient of thermal expansion values. The weight fraction of leucite was determined with quantitative x-ray diffraction using copper as an internal standard. Coefficient of thermal expansion values were determined using a thermal dilatometric analyzer. Five commercial body porcelains were studied. Leucite was not detected in samples of Vitadur N and Duceram LFC. An ANOVA showed that there was a significant difference in the weight fraction of leucite for Silhouette, Ceramco II, and Optec HSP porcelains. Linear regression revealed a correlation (R = 0.91) between weight fraction of leucite and the coefficient of thermal expansion for those samples containing leucite. Duceram LFC, which is recommended by the manufacturer for use with metals and leucite-containing porcelains, had no detectable leucite although the coefficient of thermal expansion was found to be 13.2 +/- 0.4 x 10(-6)/degrees C at 25-472 degrees C. A low glass transition temperature contributed to the high average coefficient of thermal expansion value.
Microbial Conversion of α-Ionone, α-Methylionone, and α-Isomethylionone (1988)

alpha-Ionone, alpha-methylionone, and alpha-isomethylionone were converted by Aspergillus niger JTS 191. The individual bioconversion products from alpha-ionone were isolated and identified by spectrometry and organic synthesis. The major products were cis-3-hydroxy-alpha-ionone, trans-3-hydroxy-alpha-ionone, and 3-oxo-alpha-ionone. 2,3-Dehydro-alpha-ionone, 3,4-dehydro-beta-ionone, and 1-(6,6-dimethyl-2-methylene-3-cyclohexenyl)-buten-3-one were also identified. Analogous bioconversion products from alpha-methylionone and alpha-isomethylionone were also identified. From results of gas-liquid chromatographic analysis during the fermentation, we propose a metabolic pathway for alpha-ionones and elucidation of stereochemical features of the bioconversion.
EARLY EVENTS IN OLFACTORY PROCESSING (2006)

Olfactory space has a higher dimensionality than does any other class of sensory stimuli, and the olfactory system receives input from an unusually large number of unique information channels. This suggests that aspects of olfactory processing may differ fundamentally from processing in other sensory modalities. This review summarizes current understanding of early events in olfactory processing. We focus on how odors are encoded by the activity of primary olfactory receptor neurons, how odor codes may be transformed in the olfactory bulb, and what relevance these codes may have for downstream neurons in higher brain centers. Recent findings in synaptic physiology, neural coding, and psychophysics are discussed, with reference to both vertebrate and insect model systems.
(-)-δ-N-Normethylskytanthine from Tecoma arequipensis (1988)

Bark of Tecoma arequipensis yielded the major alkaloidal component (-)-delta-N-normethylskytanthine whose structure was proven by X-ray diffraction analysis of the N-thiourea derivative. The stereochemistry of 1 at all four centers was enantiomeric with that of skytanthine previously reported from Tecoma stans and Skytanthus acutus. Gc-ms of the crude base fraction indicated the presence of several related alkaloids as very minor constituents.
Determination of paramagnetic lanthanide(III) concentrations from bulk magnetic susceptibility shifts in NMR spectra (2001)

Abstract The bulk magnetic susceptibility (BMS) shifts of 1 H NMR resonance signals for inert reference compounds caused by the addition of paramagnetic lanthanide(III) compounds were exploited in order to determine lanthanide(III) concentrations in solution. The BMS shifts of an inert reference compound in the samples were measured by comparing the chemical shifts with those of an external reference. The concentration values obtained were in good agreement with results acquired from inductively coupled plasma analysis. Copyright © 2001 John Wiley & Sons, Ltd.
Mycobacterium avium Complex Infection (1988)

Journal Article Mycobacterium avium Complex Infection Get access Lowell S. Young Lowell S. Young Kuzell Institute for Arthritis and Infectious Diseases, and the Division of Infectious Diseases, Pacific Presbyterian Medical Center, University of California, San Francisco, California Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Infectious Diseases, Volume 157, Issue 5, May 1988, Pages 863–867, https://doi.org/10.1093/infdis/157.5.863 Published: 01 May 1988
Selective activation of gamma/delta + T cell clones by single anti-CD2 antibodies. (1991)

The CD2 antigen is the target for an "alternative" T cell activation pathway. Numerous studies have demonstrated that pairs of monoclonal antibodies (mAbs) directed toward two different epitopes are required for activation of T cell receptor (TCR)-alpha/beta + T cells via CD2. We have now explored the activation of human TCR-gamma/delta + T cell clones by a panel of anti-CD2 mAbs directed against the sheep erythrocyte-binding (T11.1) epitope of CD2. Seven of seven gamma/delta + clones expressing different molecular forms of the TCR-gamma/delta responded to stimulation by a single anti-CD2 mAb (OKT11, 9E8, BW0110, M-T910) with IL-2 secretion and/or proliferation. Immobilization of anti-CD2 mAbs in microculture plates was essential for activation of gamma/delta + clones, which occurred in the absence of feeder cells. In addition to interleukin 2 (IL-2) production and proliferation, anti-CD2 mAbs also triggered cytotoxic effector activity in gamma/delta + clones as measured against FcR+ P815 target cells. In contrast to gamma/delta + clones (but in line with established data), none of five CD4+ or CD8+ TCR-alpha/beta + clones were activated by any of the tested individual anti-CD2 mAbs. Taken together, our results reveal a striking difference between cloned gamma/delta + and alpha/beta + T cells in that gamma/delta + T cells are selectively activated by a single anti-CD2 (T11.1) mAb, without need for the simultaneous signal of a second anti-CD2 mAb directed against another (T11.2 or T11.3) CD2 epitope.
PATTERN RECOGNITION: PORTABLE COMPUTER PROGRAMS FOR THE FOOD INDUSTRY (1979)

ABSTRACT Some of the available libraries of pattern recognition programs are tabulated. These include programs ARTHUR (University of Washington), RECOG (Lawrence Livermore Laboratory), and STAG (Fairfield University) the last of which uses both the APL and FORTRAN IV computer languages. The distinction between supervised and unsupervised methods of pattern recognition is presented and the more useful computer algorithms are described. These include programs and literature references for minimal spanning tree, factor analysis, linear dimension reduction, nonlinear dimension reduction, nearest neighbor, and the linear learning machine. The intent is to make the food process industry more aware of the availability of portable computer programs using that branch of artificial intelligence that has come to be termed pattern recognition.
Performance Characteristics of an Operating Supercritical CO2 Brayton Cycle (2012)

Supercritical CO2 (S-CO2) power cycles offer the potential for better overall plant economics due to their high power conversion efficiency over a moderate range of heat source temperatures, compact size, and potential use of standard materials in construction [1,2,3,4]. Sandia National Labs (Albuquerque, NM, US) and the US Department of Energy (DOE-NE) are in the process of constructing and operating a megawatt-scale supercritical CO2 split-flow recompression Brayton cycle with contractor Barber-Nichols Inc. [5] (Arvada, CO, US). This facility can be counted among the first and only S-CO2 power producing Brayton cycles anywhere in the world. The Sandia-DOE test-loop has recently concluded a phase of construction that has substantially upgraded the facility by installing additional heaters, a second recuperating printed circuit heat exchanger (PCHE), more waste heat removal capability, higher capacity load banks, higher temperature piping, and more capable scavenging pumps to reduce windage within the turbomachinery. With these additions, the loop has greatly increased its potential for electrical power generation — according to models, as much as 80 kWe per generator depending on loop configuration — and its ability to reach higher temperatures. To date, the loop has been primarily operated as a simple recuperated Brayton cycle, meaning a single turbine, single compressor, and undivided flow paths. In this configuration, the test facility has begun to realize its upgraded capacity by achieving new records in turbine inlet temperature (650°F/615K), shaft speed (52,000 rpm), pressure ratio (1.65), flow rate (2.7 kg/s), and electrical power generated (20kWe). Operation at higher speeds, flow rates, pressures and temperatures has allowed a more revealing look at the performance of essential power cycle components in a supercritical CO2 working fluid, including recuperation and waste heat rejection heat exchangers (PCHEs), turbines and compressors, bearings and seals, as well as auxiliary equipment. In this report, performance of these components to date will be detailed, including a discussion of expected operational limits as higher speeds and temperatures are approached.
3D Electrospun scaffolds promote a cytotrophic phenotype of cultured primary astrocytes (2014)

Astrocytes are a target for regenerative neurobiology because in brain injury their phenotype arbitrates brain integrity, neuronal death and subsequent repair and reconstruction. We explored the ability of 3D scaffolds to direct astrocytes into phenotypes with the potential to support neuronal survival. Poly-ε-caprolactone scaffolds were electrospun with random and aligned fibre orientations on which murine astrocytes were sub-cultured and analysed at 4 and 12 DIV. Astrocytes survived, proliferated and migrated into scaffolds adopting 3D morphologies, mimicking in vivo stellated phenotypes. Cells on random poly-ε-caprolactone scaffolds grew as circular colonies extending processes deep within sub-micron fibres, whereas astrocytes on aligned scaffolds exhibited rectangular colonies with processes following not only the direction of fibre alignment but also penetrating the scaffold. Cell viability was maintained over 12 DIV, and cytochemistry for F-/G-actin showed fewer stress fibres on bioscaffolds relative to 2D astrocytes. Reduced cytoskeletal stress was confirmed by the decreased expression of glial fibrillary acidic protein. PCR demonstrated up-regulation of genes (excitatory amino acid transporter 2, brain-derived neurotrophic factor and anti-oxidant) reflecting healthy biologies of mature astrocytes in our extended culture protocol. This study illustrates the therapeutic potential of bioengineering strategies using 3D electrospun scaffolds which direct astrocytes into phenotypes supporting brain repair. Astrocytes exist in phenotypes with pro-survival and destructive components, and their biology can be modulated by changing phenotype. Our findings demonstrate murine astrocytes adopt a healthy phenotype when cultured in 3D. Astrocytes proliferate and extend into poly-ε-caprolactone scaffolds displaying 3D stellated morphologies with reduced GFAP expression and actin stress fibres, plus a cytotrophic gene profile. Bioengineered 3D scaffolds have potential to direct inflammation to aid regenerative neurobiology.
Purification and Characterization of Trehalase Inhibitor from Hemolymph of the American Cockroach, Periplaneta americana (1989)

An endogenous proteinaceous inhibitor of trehalase (alpha,alpha-trehalose-1-glucohydrolase: EC 3.2.1.28) has been isolated and purified from the serum of resting adult American cockroaches, Periplaneta americana. Purification procedures involved decreasing ionic strength, gel filtration, and reversed phase high performance liquid chromatography. Homogeneity was confirmed by polyacrylamide gel electrophoresis and end group analysis. The purified protein inhibited trehalase activity in a dose-dependent manner and was estimated to have a molecular weight of 86,000 and to contain sugar chains. An automated gas-phase sequencer was used to determine the following sequence for the N-terminal amino acid residues: H-Ala-Ilu-Pro-Thr-Pro-His-Val-Tyr-Lys-Val-X-Val-Pro-Asp-Gly-Ala-Le u-Asn-Asp.