gebhart/patrepeval huggingface dataset:
Four different DNA helicases from calf thymus. (1992)

Using a strand displacement assay we have followed DNA helicase activities during the simultaneous isolation of several enzymes from calf thymus such as DNA polymerases alpha, delta, and epsilon, proliferating cell nuclear antigen, and replication factor A. Thus we were able to discriminate and isolate four different DNA helicases called A, B, C, and D. DNA helicase A is identical with the enzyme described earlier (Thömmes, P., and Hübscher, U. (1990) J. Biol. Chem. 265, 14347-14354). The four enzymes can be distinguished by (i) their putative molecular weights after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, (ii) glycerol gradient sedimentation under low and high salt conditions, (iii) sensitivity to salt, (iv) binding to DNA, (v) nucleoside- and deoxynucleoside 5'-triphosphate requirements, and (vi) by their direction of movement. DNA helicase A unwinds in the 3'----5' direction on the DNA it was bound to, while DNA helicases B, C, and D do so in the 5'----3' direction. DNA helicase D, and to some extent DNA helicases B and C, are able to unwind long substrates of more than 400 nucleotides. Replication factor A, a single-stranded heterotrimeric DNA binding protein involved in cellular DNA replication and DNA repair stimulates the DNA helicases. The stimulatory effect is most pronounced on DNA helicase A, where replication factor A enables this helicase to unwind longer substrates. DNA helicases B, C, and D are also stimulated by replication factor A. The effect of replication factor A appears to be specific since corresponding single-stranded DNA binding proteins from Escherichia coli and bacteriophage T4 have no or even a negative effect on the four DNA helicases. Heterologous human replication factor A has no stimulatory effect on any of the four DNA helicases suggesting a species specificity of these interactions. Thus it appears that mammalian cells possess, as does E. coli, a variety of different enzymes that can transiently abolish the double helical DNA structure in the cell.
Volitional Postures during Maximal Push/Pull Exertions in the Sagittal Plane (1983)

A set of push/pull experiments was performed by six men and women of varying anthropometry who were asked to exert maximal one-handed and two-handed push and pull forces on a load cell set at three different heights (67, 109, and 152 cm). They were each permitted to experiment with their postures to determine that which they sensed would permit the largest push or pull force, though no direct feedback was given as to their actual performance. Once they achieved what they believed to be their optimal posture, sagittal-plane photographs were taken. The angles of major body joints were then recorded along with the isometric force produced. It is shown that foot placement, handle height, and body postures affect push and pull force capability in a manner that is reasonably logical, using biomechanical concepts. It is proposed that future biomechanical models of push an pull strengths and workplace layouts must be carefully assessed with reference to the postural variations reported.
Effect of converting enzyme inhibition on glucocorticoid hypertension in the rat (1980)

The renin-angiotensin system was evaluated by inhibition of converting enzyme (CEI) and by testing sensitivity to angiotensin II (AII) in sodium-depleted rats made hypertensive by methylprednisolone (MP), 20 mg/kg im. During a 2-wk period blood pressure rose 38 +/- 4 mmHg (P less than 0.001) in MP and 3 +/- 4 mmHg in controls. After pentobarbital anesthesia, intra-arterial pressure and dose-response curves to AII were determined, before and after SQ14225 (d-3-mercapto-2-methylpropranoyl-l-proline) (1 mg/kg iv). CEI reduced pressure significantly in both MP and controls, although the decrease was smaller in the former (P less than 0.05). Pressor responses to AII were nearly identical in MP and controls and were enhanced to a similar extent by CEI. Ganglionic blockade with pentolinium tartrate, given after CEI, did reduce the pressure in both groups to equal levels. Responses to AII after pentolinium were similar to those obtained after CEI alone. These results indicate that the renin component of glucocorticoid hypertension during sodium deficiency is smaller than that of the normotensive controls. No evidence of glucocorticoid-induced vascular hypersensitivity to AII was detected in this model of experimental hypertension.
Parallel sorting in two-dimensional VLSI models of computation (1989)

The gradual refinement of a general approach to two-dimensional sorting, the shear-sort algorithm, to more sophisticated and specialized sorting algorithms on mesh-connected computers is described. The analysis of the shear-sort algorithm gives rise to a novel perspective of two-dimensional sorting, which seems to be a very powerful tool for developing efficient algorithms. The same methods can be extended for sorting in higher dimensions, for example, in the three-dimensional mesh. The concept of clean and dirty rows can be modified to clean and dirty planes (or hyperplanes for dimensions greater than three). Although only two schemes (purely recursive and iterative) are explicitly described, the reader may construct his own algorithm using similar technique and slight modifications. Designing an O(n) algorithm for sorting on a mesh becomes much simpler using the techniques developed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Artificial Homeostasis: Integrating Biologically Inspired Computing (2003)

We present a framework which integrates artificial neural networks, artificial immune systems and a novel artificial endocrine system. The natural counterparts of these three components are usually assumed to be the principal actors in maintaining homeostasis within biological systems. This paper proposes a system which promises to capitalise on the self-organizing properties of these artificial systems to yield artificially homeostatic systems. The components develop in a common environment and interact in ways which draw heavily on their biological counterparts for inspiration. Key words: Homeostasis, wibble 1
Formation of a Gel from a Heated Emulsion of Alfalfa Leaf Protein and Peanut Oil (1987)

ABSTRACT A new method was investigated for extracting the proteins from green leaves (alfalfa). An opaque white gel formed when an emulsion of chloroplast‐free leaf tissue juice and peanut oil was heated for 10 min at 90°C. The proximate composition of this gel was 40% moisture, 2% protein, 57% fat, 0.2% ash, and <1% carbohydrate. SDS‐PAGE analysis of the gel suggested that fraction 1 or ribulose bisphosphate carboxylase is the major protein component present. The apparent viscosity of the gel, 5,257 m Pa.sec, was less than one‐tenth of mayonnaise.
Glass microfluidic devices with thin membrane voltage junctions for electrospray mass spectrometry (2005)

In this study a novel glass membrane was prepared for conducting high voltage (HV) to solution in the channel of a microfabricated device for generation of liquid electrospray. Taylor cone formation and mass spectra obtained from this microdevice confirmed the utility of the glass membrane, but voltage conduction through the membrane could not be successfully explained based solely on the conductivity of the glass itself. This novel method for developing a high-voltage interface for microdevices avoids direct metal/liquid contact eliminating bubble formation in the channel due to water hydrolysis on the surface of the metal. Further, this arrangement produces no dead volume as is often found with traditional liquid junctions. At the same time, preliminary investigations into the outlet design of glass microdevices for interfacing with electrospray mass spectrometry, was explored. Both the exit shape and the use of hydrophobic coatings at the channel exit of the microdevice electrospray interface were evaluated using standard proteins with results indicating the utility of this type of design after further optimization.
A Useful Modification of theEvans Auxiliary: 4-Isopropyl-5,5-diphenyloxazolidin-2-one (1998)

The 4-isopropyl-5,5-diphenyloxazolidinone (1) is readily prepared from (R)- or (S)-valine ester, PhMgBr, and ethyl chlorocarbonate. It has a melting point of ca. 250°, a low solubility in most organic solvents, and a C=O group which is sterically protected from nucleophilic attack. Thus, the soluble N-acyl-oxazolidinones (7 – 16) can be prepared from 1 with BuLi at temperatures around 0° instead of −78° (Scheme 3), their Li enolates can be generated with BuLi, rather than with LDA, and deacylation in the final step of the procedure can be achieved with NaOH at ambient temperatures (Scheme 12), with facile recovery of the precipitating auxiliary 1 (filtering, washing, and drying). The following reactions of N-acyl-oxazolidinones from 1 have been investigated: alkylations (Scheme 4), aminomethylations and hydroxymethylations (Scheme 5), aldol additions (Schemes 6 and 7), Michael additions (Schemes 9 and 10), and a (4+2) cycloaddition (Scheme 11). The well-known features of reactions following the Evans methodology (yield, diastereoselectivity, dependence on conditions, counter ions, additives etc.) prevail in these transformations. Most products, however, have higher melting points and a much more pronounced crystallization tendency than those derived from conventional oxazolidinones, and can thus be purified by recrystallization, avoiding chromatography (Table 1). The disadvantage of 1 having a higher molecular weight (ca. 150 Da) than the non-phenyl-substituted auxiliary is more than compensated by the ease of its application, especially on large scale. A number of crystal structures of oxazolidinones derived from 1 and a TiCl4 complex of an oxazolidinone are described and discussed in view of the diastereoselective-reaction mechanisms.
Cost-effectiveness analysis of education and health interventions in developing countries (2012)

High-quality impact evaluations, including randomised experiments, are increasingly popular, but cannot always inform resource allocation decisions unless the costs of interventions are considered alongside their effects. Cost-effectiveness analysis is a straightforward but under-utilised tool for determining which of two or more interventions provides a (non-pecuniary) unit of effect at least cost. This paper reviews the framework and methods of cost-effectiveness analysis, emphasising education and health interventions, and discusses how the methods are currently applied in the literature.
Point-of-Care Troponin Testing in Patients With Symptoms Suggestive of Acute Coronary Syndrome: A Health Technology Assessment (2016)

Testing of cardiac biomarkers, such as cardiac troponin I or cardiac troponin T, has an important role in the diagnostic workup for acute coronary syndrome (ACS) (including acute myocardial infarction [AMI] and unstable angina), and in patients presenting with acute chest pain and a non-diagnostic electrocardiogram (ECG). Bedside testing of cardiac troponins (cTn) using point-of-care (POC) assays was developed to reduce the turnaround time of the standard tests performed in a central laboratory, and to expedite treatment. Given the introduction and increasing diffusion of POC cTn use, a review of its clinical and economic evidence is needed to inform decisions about its acquisition and use in emergency rooms and other in-hospital settings, as well as in rural health care centres and remote settings.
The Multiple Functions of Hemoglobin (1995)

The aim of this review is to focus and discuss several parallel biological functions of hemoglobin besides its basic function of oxygen transport. In light of the information present in the literature the following possible physiological roles of hemoglobin are discussed: (1) hemoglobin as molecular heat transducer through its oxygenation-deoxygenation cycle, (2) hemoglobin as modulator of erythrocyte metabolism, (3) hemoglobin oxidation as an onset of erythrocyte senescence, (4) hemoglobin and its implication in genetic resistance to malaria, (5) enzymatic activities of hemoglobin and interactions with drugs, and (6) hemoglobin as source of physiological active catabolites.
Development Of A Gas Handling Hydraulic Submersible Pump And Planning A Field Trial, Captain Field (1997)

Abstract There are limitations to the amount of free gas that can be handled by high volume downhole pump systems. A new pump design employing axial flow impellers coupled with high speed downhole turbine drive has been developed, and is the first true downhole multiphase pump. This paper describes the staged research and development program leading to the first field trial installation in the Captain Field, in the North Sea. Development and design requirements are reviewed, with the results from laboratory testing in water and nitrogen followed by full scale loop testing in multiphase fluid, including crude, methane and brine. Test configuration is described, and key performance results reviewed. Special testing defined the nature of fluids and rheology related to high rotational speed pumping of viscous crude and brine mixtures in pumps and pipelines. Following successful laboratory scale testing, design considerations are reviewed for its application in a field trial. Completion design includes provision for open loop flow and access below the pump assembly for coiled tubing intervention. New downhole monitoring systems employ pump and turbine monitors along with pump flow and reservoir evaluation monitoring. The design results in extended range of pump operability, improved reliability, and improved control and reservoir management. Introduction As technology improves in horizontal drilling, completion practices, and subsea equipment, the need for high volume downhole pumping systems has increased. Small accumulations or thin oil zones, previously considered marginal, can be exploited economically. But in some applications, pumping systems needed higher gas handling capability because maximizing recovery from individual wells requires larger pressure drawdown. Additionally, horizontal wells located at the top of the productive intervals can encounter free gas caps. Development of a downhole pumping system that could operate successfully in a gassy environment (higher than 30% gas void fraction), and deliver high reliability was key to exploiting new reserves. This technology could be applied within many major oil compani's existing asset portfolio, such as the Gulf of Mexico or West of Shetlands deepwater environments, and elsewhere in the North Sea. One of the assets that could benefit from this technology is the Captain Field. Although the main area of the field is being developed with Electrical Submersible Pumps (ESP), an isolated gas cap was identified in the eastern section of the field that would limit the reliability of ESP systems, and would limit the withdrawal rate. The field has 19 degree API crude oil, with 88 centipoise (CP) viscosity at reservoir pressure of 1300 psi and 87 deg F.
Three cases of factor I deficiency: the effect of treatment with plasma. (1988)

Three patients with congenital factor I deficiency associated with different clinical manifestations are described. Case 1 had one single episode of meningococcal disease, case 2 experienced four episodes of meningococcal disease and several other severe infections, whereas case 3, without known predisposition for infections, died from a subacute immune-complex mediated syndrome, resembling polyarteritis nodosa. Family studies in cases 1 and 2 revealed healthy individuals with factor I concentrations below the lower reference limit, indicating heterozygous carriers. The pedigree analyses were consistent with autosomal codominant inheritance. The estimated minimal frequency of the deficient gene was 0.002. Pedigree analysis was not performed in case 3 but the father and sister was found to be probable heterozygous carriers. Cases 2 and 3 were treated with infusions of freshly frozen plasma (FFP) (40 and 27 ml/kg bodyweight) during acute illness and the immunochemical complement profile was monitored. Following plasma infusion factor I was cleared from the circulation with a half-life of 29-45 h. The plasma infusions induced generation of C3d and C4d, increase in native factor B and C3 concentrations and disappearance of Ba split products. Native C3 and C4 increased to normal concentrations and remained normal till 16 days after the plasma infusions, whereas native factor B decreased to preinfusion levels 8 days after plasma infusion. It is concluded, that congenital factor I deficiency can present with different clinical manifestations and may be more prevalent than hitherto anticipated. Furthermore, infusion of blood products containing small amounts of functional factor I can partly normalize the complement profile, with a more prolonged effect on C3 and C4 than on factor B metabolism.
Inhibitory Effects of WR-2721 and Cysteamine on Tumor Initiation in Mammary Glands of Pregnant Rats by Radiation (2000)

We evaluated the effect of WR-2721 [S-2-(3-aminopropylamino)-ethylphosphorothioic acid] and cysteamine (2-mercaptoethylamine) on the development of radiation-induced mammary tumors in rats. Pregnant rats were treated with WR-2721 or cysteamine 30 min prior to whole-body irradiation with gamma rays from a (60)Co source at a dose of 1.5 or 2.6 Gy. Additional pregnant rats were given saline and then exposed to gamma rays at a dose of 0, 1.5 or 2.6 Gy as a control. All rats were implanted with pellets of diethylstilbestrol, a tumor promoter, 1 month after termination of nursing and were observed for 1 year to detect palpable mammary tumors. No mammary tumors developed in the saline-injected nonirradiated rats. However, when rats were irradiated with 1.5 or 2. 6 Gy after saline treatment, the incidence of mammary tumors was high (71.4 and 92.3%, respectively). Administration of WR-2721 or cysteamine prior to irradiation with 1.5 Gy significantly decreased the tumor incidence (23.8 and 20.8%, respectively). Tumor prevention by either agent was less effective at the higher dose. The appearance of the first mammary tumor occurred later in rats treated with WR-2721 or cysteamine than in the control rats. An increasing rate of adenocarcinoma in the control group was observed with increasing dose from 1.5 Gy up to 2.6 Gy. However, the development of adenocarcinoma did not increase after pretreatment with WR-2721 or cysteamine in rats irradiated with 2.6 Gy. Many of the mammary tumors that developed in the control rats were of the ER(+)PgR(+) type. Administration of WR-2721 produced no tumors of the ER(+)PgR(+) type. Cysteamine treatment increased the development of ER-negative tumors. The serum concentration of progesterone was significantly higher in rats treated with WR-2721 or cysteamine than in the control rats. On the other hand, the estradiol-17beta concentration was reduced by treatment with WR-2721, but not significantly compared to the control. WR-2721 and cysteamine had no effect on the prolactin concentration of the irradiated rats. The results suggest that administration of WR-2721 or cysteamine prior to the irradiation has a potent preventive effect on theinitiation phase during mammary tumorigenesis.
Topical Corticosteroids of Limited Potency Promote Adenovirus Replication in the Ad5/NZW Rabbit Ocular Model (2002)

Treatment of an experimental ocular adenovirus infection with PA, FM, and RMX for 3 days significantly enhanced adenovirus replication compared with the control group. Short-term treatment of EKC with several commercially available topical corticosteroids with limited potency may offer symptomatic relief, but may also delay viral clearance and promote office and community epidemics.
Efficacy and Tolerance of Sodium Phosphates Oral Solution After Diet Liberalization (2005)

Bowel cleansing regimens commonly require adherence to liquid diets for 24 to 48 hours before examination, which often leads to poor compliance, reduced cleansing, and ultimately inadequate examinations. The authors investigated the efficacy and tolerability of diet liberalization before bowel cleansing with sodium phosphates oral solution. Two hundred patients were randomized into two treatment groups. One group received the standard light breakfast followed by clear liquids the day before colonoscopy; the second had a normal breakfast followed by a low-residue lunch the day before colonoscopy. Both groups had the same bowel preparation with sodium phosphates oral solution (2 x 45-mL, 7 p.m./6 a.m.). There was no difference in clinical efficacy between the two diet regimens (excellent/good in 93% standard, 95% low-residue). Fewer patients receiving the low-residue diet reported hunger, and more patients receiving the low-residue regimen reported energy to perform usual activities. This study supports offering patients a regular breakfast and a low-residue lunch before bowel cleansing with sodium phosphates oral solution.
Early diagnosis of Alzheimer’s disease using combined features from voxel-based morphometry and cortical, subcortical, and hippocampus regions of MRI T1 brain images (2019)

In recent years, several high-dimensional, accurate, and effective classification methods have been proposed for the automatic discrimination of the subject between Alzheimer's disease (AD) or its prodromal phase {i.e., mild cognitive impairment (MCI)} and healthy control (HC) persons based on T1-weighted structural magnetic resonance imaging (sMRI). These methods emphasis only on using the individual feature from sMRI images for the classification of AD, MCI, and HC subjects and their achieved classification accuracy is low. However, latest multimodal studies have shown that combining multiple features from different sMRI analysis techniques can improve the classification accuracy for these types of subjects. In this paper, we propose a novel classification technique that precisely distinguishes individuals with AD, aAD (stable MCI, who had not converted to AD within a 36-month time period), and mAD (MCI caused by AD, who had converted to AD within a 36-month time period) from HC individuals. The proposed method combines three different features extracted from structural MR (sMR) images using voxel-based morphometry (VBM), hippocampal volume (HV), and cortical and subcortical segmented region techniques. Three classification experiments were performed (AD vs. HC, aAD vs. mAD, and HC vs. mAD) with 326 subjects (171 elderly controls and 81 AD, 35 aAD, and 39 mAD patients). For the development and validation of the proposed classification method, we acquired the sMR images from the dataset of the National Research Center for Dementia (NRCD). A five-fold cross-validation technique was applied to find the optimal hyperparameters for the classifier, and the classification performance was compared by using three well-known classifiers: K-nearest neighbor, support vector machine, and random forest. Overall, the proposed model with the SVM classifier achieved the best performance on the NRCD dataset. For the individual feature, the VBM technique provided the best results followed by the HV technique. However, the use of combined features improved the classification accuracy and predictive power for the early classification of AD compared to the use of individual features. The most stable and reliable classification results were achieved when combining all extracted features. Additionally, to analyze the efficiency of the proposed model, we used the Alzheimer's Disease Neuroimaging Initiative (ADNI) dataset to compare the classification performance of the proposed model with those of several state-of-the-art methods.