gebhart/patrepeval huggingface dataset:
Mitochondrial function as a determinant of life span (2009)

Average human life expectancy has progressively increased over many decades largely due to improvements in nutrition, vaccination, antimicrobial agents, and effective treatment/prevention of cardiovascular disease, cancer, etc. Maximal life span, in contrast, has changed very little. Caloric restriction (CR) increases maximal life span in many species, in concert with improvements in mitochondrial function. These effects have yet to be demonstrated in humans, and the duration and level of CR required to extend life span in animals is not realistic in humans. Physical activity (voluntary exercise) continues to hold much promise for increasing healthy life expectancy in humans, but remains to show any impact to increase maximal life span. However, longevity in Caenorhabditis elegans is related to activity levels, possibly through maintenance of mitochondrial function throughout the life span. In humans, we reported a progressive decline in muscle mitochondrial DNA abundance and protein synthesis with age. Other investigators also noted age-related declines in muscle mitochondrial function, which are related to peak oxygen uptake. Long-term aerobic exercise largely prevented age-related declines in mitochondrial DNA abundance and function in humans and may increase spontaneous activity levels in mice. Notwithstanding, the impact of aerobic exercise and activity levels on maximal life span is uncertain. It is proposed that age-related declines in mitochondrial content and function not only affect physical function, but also play a major role in regulation of life span. Regular aerobic exercise and prevention of adiposity by healthy diet may increase healthy life expectancy and prolong life span through beneficial effects at the level of the mitochondrion.
Efficient Degradation of Toxic Organic Pollutants with Ni<sub>2</sub>O<sub>3</sub>/TiO<sub>2</sub><sub>-</sub><i><sub>x</sub></i>B<i><sub>x</sub></i> under Visible Irradiation (2004)

To promote efficient use of solar energy, many studies have focused on the modification of TiO2 to extend its spectral response to visible region. Here we report a combined modification of TiO2 by two components: the nonmetal element boron and the metal oxide Ni2O3. The photocatalyst presents high photocatalytic activity in the visible region, which can efficiently degrade and mineralize toxic organic pollutants such as trichlorophenol (TCP), 2,4-dichlorophenol (2,4-DCP), and sodium benzoate. The dechlorination and mineralization results indicate the photocatalytic pathway via visible light excitation. The study demonstrates that the modification of TiO2 both to extend its spectral response to the visible region and to improve its catalytic efficiency can be achieved by doping with boron, a nonmetal, and Ni2O3, a metal oxide.
V-NetLab: an approach for realizing logically isolated networks for security experiments (2008)

Cyber security experiments with potentially malicious software can possibly damage the testbed environment and escape into the Internet. Due to this security con-cern, networks used in such experiments are often to-tally isolated from production networks and the Inter-net. This choice, however, precludes remote access to testbeds used for security experiments, thus requiring costly duplication of equipment, manpower and expertise at sites that experiment with malicious software. We pro-pose an alternative approach that is aimed at providing a degree of safety comparable to that of physically iso-lated testbeds while still permitting remote connectivity. Our approach relies on logical isolation of networks used in different security experiments using network virtual-ization at the datalink layer. We have implemented this approach into a platform (V-NetLab), and the responses from testbed users have been very positive. 1
The pCLEAN Dual Binary Vector System for<i>Agrobacterium</i>-Mediated Plant Transformation (2007)

The development of novel transformation vectors is essential to the improvement of plant transformation technologies. Here, we report the construction and testing of a new multifunctional dual binary vector system, pCLEAN, for Agrobacterium-mediated plant transformation. The pCLEAN vectors are based on the widely used pGreen/pSoup system and the pCLEAN-G/pCLEAN-S plasmids are fully compatible with the existing pGreen/pSoup vectors. A single Agrobacterium can harbor (1) pCLEAN-G and pSoup, (2) pGreen and pCLEAN-S, or (3) pCLEAN-G and pCLEAN-S vector combination. pCLEAN vectors have been designed to enable the delivery of multiple transgenes from distinct T-DNAs and/or vector backbone sequences while minimizing the insertion of superfluous DNA sequences into the plant nuclear genome as well as facilitating the production of marker-free plants. pCLEAN vectors contain a minimal T-DNA (102 nucleotides) consisting of direct border repeats surrounding a 52-nucleotide-long multiple cloning site, an optimized left-border sequence, a double left-border sequence, restriction sites outside the borders, and two independent T-DNAs. In addition, selectable and/or reporter genes have been inserted into the vector backbone sequence to allow either the counter-screening of backbone transfer or its exploitation for the production of marker-free plants. The efficiency of the different pCLEAN vectors has been assessed using transient and stable transformation assays in Nicotiana benthamiana and/or Oryza sativa.
A 13.56-Mbps Pulse Delay Modulation Based Transceiver for Simultaneous Near-Field Data and Power Transmission (2014)

A fully-integrated near-field wireless transceiver has been presented for simultaneous data and power transmission across inductive links, which operates based on pulse delay modulation (PDM) technique. PDM is a low-power carrier-less modulation scheme that offers wide bandwidth along with robustness against strong power carrier interference, which makes it suitable for implantable neuroprosthetic devices, such as retinal implants. To transmit each bit, a pattern of narrow pulses are generated at the same frequency of the power carrier across the transmitter (Tx) data coil with specific time delays to initiate decaying ringing across the tuned receiver (Rx) data coil. This ringing shifts the zero-crossing times of the undesired power carrier interference on the Rx data coil, resulting in a phase shift between the signals across Rx power and data coils, from which the data bit stream can be recovered. A PDM transceiver prototype was fabricated in a 0.35- μm standard CMOS process, occupying 1.6 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> . The transceiver achieved a measured 13.56 Mbps data rate with a raw bit error rate (BER) of 4.3×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-7</sup> at 10 mm distance between figure-8 data coils, despite a signal-to-interference ratio (SIR) of -18.5 dB across the Rx data coil. At the same time, a class-D power amplifier, operating at 13.56 MHz, delivered 42 mW of regulated power across a separate pair of high-Q power coils, aligned with the data coils. The PDM data Tx and Rx power consumptions were 960 pJ/bit and 162 pJ/bit, respectively, at 1.8 V supply voltage.
Detecting Acute Coronary Syndrome in the Emergency Department With Cardiac Magnetic Resonance Imaging (2003)

Resting cardiac MRI exhibited diagnostic operating characteristics suitable for triage of patients with chest pain in the emergency department. Performed urgently to evaluate chest pain, MRI accurately detected a high fraction of patients with acute coronary syndrome, including patients with enzyme-negative unstable angina.
New Exact Traveling Wave Solutions for Compound KdV-Burgers Equations in Mathematical Physics ∗† (2002)

With the aid of Mathematica, new explicit and exact travelling wave and solitary solutions for compound KdV-Burgers equations are obtained by using an improved sine-cosine method and the Wu elimination method.
Mobile Station Location Based on Hybrid of Signal Strength and Time of Arrival (2005)

Positioning of users or mobile station (MS) in cellular systems becomes an interesting topic since the related number of applications for the location information is growing rapidly. The decision to make the locating of mobile phones under the US Federal Communications Commission (FCC) mandatory is one of the driving forces to research and provide solutions to it. Deploying such applications would require a proper method for positioning the MS that are simple and accurate especially in urban area. There are many research papers addressing this issue based on timing method that calculate the timing delay for signal sent from MS to base station (BS) or vise versa, and other method based on signal strength. In this paper, we present a new calculation method to locate the mobile phone in urban district. This technique is based on hybrid method that uses signal strength to estimate the timing delay and comparing it with the measured time of arrival of the signal. The minimum timing value of the two results would be accepted and averaged for n samples. This will improve the accuracy and reduce the effect of multi-path signaling that both timing signaling and signal strength methods suffer from. In addition, a new calculation method has been presented to find the location of MS based on the BSs coordinates.
Cotunneling Transport and Quantum Phase Transitions in Coupled Josephson-Junction Chains with Charge Frustration (1998)

We investigate the quantum phase transitions in two capacitively coupled chains of ultrasmall Josephson junctions, where the particle-hole symmetry is broken by the gate voltage applied to each superconducting island. Near the maximal-frustration line, cotunneling of the particles along the two chains is shown to play a major role in the transport and to drive a quantum phase transition out of the charge-density-wave insulator, as the Josephson-coupling energy is increased. We also argue briefly that slightly off the symmetry line the universality class of the transition remains the same as that right on the line, being driven by the particle-hole pairs.
Inductive Electron Heating Revisited (1996)

The induced rf magnetic fields of low-frequency inductively coupled plasmas are measured and modeled [M. Tuszewski, Phys. Rev. Lett. 77, 1286 (1996)]. The fields penetrate deep into the discharges, in contrast with existing predictions of field decay within a thin skin layer. Fluid calculations suggest that the enhanced rf penetration is due to a reduction of the plasma conductivity by the induced magnetic fields. In this paper, new oxygen and argon data concerning rf power absorption are reported and a general description of inductive electron heating is suggested.
An aqueous sol–gel route to prepare organic–inorganic hybrid materials (2007)

A new aqueous sol–gel process has been developed that makes it possible to produce a transparent organic–inorganic hybrid material with desirable mechanical properties for several applications. During the process an aqueous precursor is prepared by adding water into a mixture of an epoxy organofunctional silane with an aluminium alkoxide. The alkoxide is then hydrolyzed and the resultant hydrate is peptized to a clear transparent solution. This process is different from the previously reported Yoldas process (B. E. Yoldas, Am. Ceram. Soc. Bull., 1975, 54, 286; 289; J. Mater. Sci., 1975, 10, 1856), which involves the addition of acid to peptize the aluminium hydrate to boehmite nanoparticles. TEM studies have shown that a molecular precursor is formed. Another interesting feature of this process is that a large amount of water is used as the solvent. This is opposite to the conventional wisdom that the water should be strictly controlled in systems similar to those reported here. Monolith and coating hybrid materials can be easily made from the synthesized precursor solutions. They showed good mechanical properties when cured at temperatures as low as 80 °C.
Purification and Structural Characterization of Bovine Cathelicidins, Precursors of Antimicrobial Peptides (1996)

Cathelicidins are a novel family of antimicrobial peptide precursors from mammalian myeloid cells. They are characterized by a conserved N-terminal region while the C-terminal antimicrobial domain can vary considerably in both primary sequence and length. Four cathelicidins, proBac5, proBac7, prododecapeptide and proBMAP-28, have been concurrently purified from bovine neutrophils, using simple and rapid methodologies. The correlation of ES-MS data from the purified proteins with their cDNA-deduced sequences has revealed several common features of their primary sequence, such as the presence of N-terminal 5-oxoproline (pyroglutamate) residues and two disulfide bridges in a 1-2, 3-4 arrangement. The N-terminal domains of the cathelicidins present one or two Asp-Pro bonds, which are particularly acid-labile in proBac5 and proBac7, but stable in prododecapeptide. This suggests that the spatial organization around these bonds may vary in different cathelicidins, and favour hydrolysis in some cases. An unexpected feature of the prododecapeptide is that it exists as dimers formed by three possible combinations of its two isoforms. The isolation of a truncated, monomeric form of this protein, lacking the cysteine-containing antimicrobial dodecapeptide, indicates that dimerization occurs via disulfide bridge formation at the level of the C-terminal domain and that the dodecapeptide is likely released as a dimer from its precursor. Sequence-based secondary structure predictions and CD results indicate for cathelicidins a 30-50% content of extended conformation and <20% content of alpha-helical conformation, with the alpha-helical segment placed near the N-terminus. Finally, similarity searching and topology-based structure prediction underline a significant sequential and structural similarity between the conserved N-terminal domain of cathelicidins and cystatin-like domains, placing this family within the cystatin superfamily. When assayed against cathepsin L, unlike the potent cystatin inhibitors, three of the four cathelicidins show only a poor inhibitory activity (Ki = 0.6-3 microM).
Integrated Starch Wet Milling Process (1974)

Abstract Optimum performance and most efficient operation in the corn wet milling process requires total integration of all equipment. Each item of process equipment relates to the total system, each item has to have specific capabilities to meet the total system requirements. The authors describe with the help of flow sheets and material balances the interdependence of each milling step and equipment with special emphasis on the centrifugal equipment, its design, and performance characteristics.
Anticancer and chemosensitizing abilities of cycloviolacin O2 from <i>Viola odorata</i> and psyle cyclotides from <i>Psychotria leptothyrsa</i> (2010)

Cycloviolacin O2 (CyO2), a cyclotide from Viola odorata (Violaceae) has antitumor effects and causes cell death by membrane permeabilization. In the breast cancer line, MCF-7 and its drug resistant subline MCF-7/ADR, the cytotoxic effects of CyO2 (0.2-10 microM) were monitored in the presence and absence of doxorubicin (0.1-5 microM) using cell proliferation assays to establish its chemosensitizing abilities. SYTOX Green assays were Sperformed to verify membrane permeabilization and showed cellular disruption correlates with cyclotide chemosensitization. Fluorescence microscopy studies demonstrated increased cellular internalization of doxorubicin in drug resistant cells when coexposed to CyO2. Interestingly, CyO2 did not produce significant membrane disruption in primary human brain endothelial cells, which suggested cyclotide specificity toward induced pore formation in highly proliferating tumor cells. Furthermore, three novel cyclotides (psyle A, C and E) from Psychotria leptothyrsa (Rubiaceae) were also monitored for cytotoxic activity. The cyclotides displayed potent cytotoxicity (IC50 = 0.64->10 microM), and coexposure to cyclotides significantly enhanced doxorubicin induced toxicity (IC50 = 0.39-0.76 microM). This study documents several cyclotides with robust cytotoxicity that may be promising chemosensitizing agents against drug resistant breast cancer.
[Study on morphological classification and chemical-type of Perilla frutescens cultivated germplasm]. (2019)

Fifty cultivated Perilla seeds were collected all over the country and planted in Beijing experiment field for morphology and chemical-type researches. Twenty morphological characteristics were selected and observed, and the essential oil from leaves was extracted by steam distillation and analyzed by GC-MS to confirm chemical-types. There were significant diversities in plant height, leaf color and morphology, and fruit color and weight. Clustering analysis was carried out based on these morphological characteristics. Six types were divided with their chemical-type designated. Type Ⅰ: Six germplasms, attributed to P. frutescens var. crispa, with dwarf plants, thin creased purple leaf, named Crispa, their chemical types were diversified, including EK, PAPK, PA and PK. Type Ⅱ: Six germplasms, attributed to P. frutescens var. crispa, plants were taller than type I and with thin and creased green leaf, named Big Crispa, all PK type. Type Ⅲ: Seventeen germplasms, attributed to P. frutescens var. frutescens with leaf color upside green and underside purple, tall plant and wide distribution all over the China, named Ordinary Frutescens, all PK. Type Ⅳ: Four germplasms, attributed to P. frutescens var. acuta with tall plant and small seed, named Acuta, all PK. Type Ⅴ: Seven germplasms, attributed to P. frutescens var. frutescens with green leaves, tall plants and long clusters, named Long-spike Frutescens, all PK. Type Ⅵ: Ten germplasms, attributed to P. frutescens var. frutescens with big, thick and creased leaf, named Thick-leaf Frutescens, including PK, PP, PL and PA. The morphological classification of this paper would lay the foundation for the taxonomic naming and following evaluation of the Perilla germplasm resources.This study also showed that there was no correspondence but a certain correlation between volatile oil chemical-types and subspecies classification and morphological characteristics of Perilla.
New World and Old World Alphaviruses Have Evolved to Exploit Different Components of Stress Granules, FXR and G3BP Proteins, for Assembly of Viral Replication Complexes (2016)

The positive-strand RNA viruses initiate their amplification in the cell from a single genome delivered by virion. This single RNA molecule needs to become involved in replication process before it is recognized and degraded by cellular machinery. In this study, we show that distantly related New World and Old World alphaviruses have independently evolved to utilize different cellular stress granule-related proteins for assembly of complexes, which recruit viral genomic RNA and facilitate formation of viral replication complexes (vRCs). Venezuelan equine encephalitis virus (VEEV) utilizes all members of the Fragile X syndrome (FXR) family, while chikungunya and Sindbis viruses exploit both members of the G3BP family. Despite being in different families, these proteins share common characteristics, which determine their role in alphavirus replication, namely, the abilities for RNA-binding and for self-assembly into large structures. Both FXR and G3BP proteins interact with virus-specific, repeating amino acid sequences located in the C-termini of hypervariable, intrinsically disordered domains (HVDs) of viral nonstructural protein nsP3. We demonstrate that these host factors orchestrate assembly of vRCs and play key roles in RNA and virus replication. Only knockout of all of the homologs results in either pronounced or complete inhibition of replication of different alphaviruses. The use of multiple homologous proteins with redundant functions mediates highly efficient recruitment of viral RNA into the replication process. This independently evolved acquisition of different families of cellular proteins by the disordered protein fragment to support alphavirus replication suggests that other RNA viruses may utilize a similar mechanism of host factor recruitment for vRC assembly. The use of different host factors by alphavirus species may be one of the important determinants of their pathogenesis.
A new meteor echo detection and collection system: Christmas Island mesospheric wind measurements (1990)

A second generation meteor echo detection and collection system has been installed on the Christmas Island, Republic of Kiribati, stratosphere‐troposphere radar system. Data from the first 6 months of operation have yielded mean wind and tidal climatologies that are compared with theoretical models.
Building Robota, a Mini-Humanoid Robot for the Rehabilitation of Children With Autism (2007)

The Robota project constructs a series of multiple-degrees-of-freedom, doll-shaped humanoid robots, whose physical features resemble those of a human baby. The Robota robots have been applied as assistive technologies in behavioral studies with low-functioning children with autism. These studies investigate the potential of using an imitator robot to assess children's imitation ability and to teach children simple coordinated behaviors. In this article, the authors review the recent technological developments that have made the Robota robots suitable for use with children with autism. They critically appraise the main outcomes of two sets of behavioral studies conducted with Robota and discuss how these results inform future development of the Robota robots and robots in general for the rehabilitation of children with complex developmental disabilities.
Electronic transport in monolayer graphene nanoribbons produced by chemical unzipping of carbon nanotubes (2009)

We report on the structural and electrical properties of graphene nanoribbons (GNRs) produced by the oxidative unzipping of carbon nanotubes. GNRs were reduced by hydrazine at 95 °C and further annealed in Ar/H2 at 900 °C; monolayer ribbons were selected for the fabrication of electronic devices. GNR devices on Si/SiO2 substrates exhibit an ambipolar electric field effect typical for graphene. The conductivity of monolayer GNRs (∼35 S/cm) and mobility of charge carriers (0.5–3 cm2/V s) are less than the conductivity and mobility of pristine graphene, which could be explained by oxidative damage caused by the harsh H2SO4/KMnO4 used to make GNRs. The resistance of GNR devices increases by about three orders of magnitude upon cooling from 300 to 20 K. The resistance/temperature data is consistent with the variable range hopping mechanism, which, along with the microscopy data, suggests that the GNRs have a nonuniform structure.