gebhart/patrepeval huggingface dataset:
FGF-2 induced reorganization and disruption of actin cytoskeleton through PI 3-kinase, Rho, and Cdc42 in corneal endothelial cells. (2003)

These data indicate that CECs in culture have constitutively active Rho activity as evidenced by stress fiber formation and that PI 3-kinase negatively regulates the formation of stress fibers and focal adhesions, perhaps antagonizing the Rho pathways. Formation of pseudopodia in response to FGF-2 and Y27632 may suggest that the Rho/ROCK pathway negatively regulates Cdc42.
Structural and cognitive problems in providing version control for hypertext (1993)

Article Free Access Share on Structural and cognitive problems in providing version control for hypertext Author: Kasper Østerbye View Profile Authors Info & Claims ECHT '92: Proceedings of the ACM conference on HypertextNovember 1992 Pages 33–42https://doi.org/10.1145/168466.168484Published:01 December 1993Publication History 44citation387DownloadsMetricsTotal Citations44Total Downloads387Last 12 Months10Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Diurnal Variation in Mood and Performance in a Time-Isolated Environment (1985)

In order to document circadian rhythmicity in various psychological functions under the chronobiologically 'pure' condition of temporal isolation, a battery of mood and performance tests were administered about 6 times per day to a heterogeneous group of 18 subjects (ages 19-81, 5 female). Each subject spent about 5 days in temporal isolation, entrained to a routine equivalent to his/her own habitual sleep/wake cycle. Average time of day functions were obtained for the mood and performance variables, and compared to rectal temperature data subjected to exactly the same statistical analysis. Significant time of day effects were found in the mood variables of alertness, sleepiness, weariness, effort required, happiness and well-being. Times of 'best' mood were different from the time of peak temperature. Moreover, the minima of sleepiness, weariness and effort occurred earlier in the day than the maximum of alertness. Significant time of day effects were also found in the speed with which search and dexterity tasks were completed. Only the dexterity tasks showed a complete parallelism with the temperature rhythm.
Powder X-Ray Diffraction and Infrared Investigation of the Product of the Chromium(VI) Oxide-Liquid Ammonia Reaction (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPowder X-Ray Diffraction and Infrared Investigation of the Product of the Chromium(VI) Oxide-Liquid Ammonia ReactionRussell S. Drago and Harry H. SislerCite this: J. Am. Chem. Soc. 1957, 79, 8, 1811–1815Publication Date (Print):April 1, 1957Publication History Published online1 May 2002Published inissue 1 April 1957https://pubs.acs.org/doi/10.1021/ja01565a012https://doi.org/10.1021/ja01565a012research-articleACS PublicationsRequest reuse permissionsArticle Views66Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Microcavity hydrogen storage. Final progress report (1981)

In the microcavity storage system, high pressure hydrogen is stored in hollow, glass microspheres, 5 to 150 ..mu..m. This report presents the results of an experimental study to evaluate the performance of commercially available microspheres for this application. Eight grades were evaluated and their characteristics are presented. A substantial fraction of the microsphere beds survived the conditions of storing hydrogen at pressures of 400 atm. establishing that the concept of high pressure hydrogen storage is feasible. Information was gathered on the properties of the survivor microspheres. Processes for their selective recovery are being investigated.
Kinetics of monocyte 1α-hydroxylase in renal failure (1995)

In chronic uremia, the requirement of supraphysiological doses of serum 25-hydroxyvitamin D 3 [25(OH)D 3 ] for the normalization of 1,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] levels has been attributed to impaired substrate availability to renal 1α-hydroxylase. Because serum 1,25(OH) 2 D 3 can also be corrected by 25(OH)D 3 supplementation in bilaterally nephrectomized patients, we examined the role of substrate availability on 1,25(OH) 2 D 3 production by peripheral blood monocytes (PBM). In hemodialysis patients (HP), 25(OH)D 3 uptake was 50% lower than normal, and the maximal velocity (V max ) and apparent Michaelis constant (K m ) for 25(OH)D 3 of 1α-hydroxylase were 2.7- and 4-fold above normal, respectively. When serum 1,25(OH) 2 D 3 of HP was corrected by intravenous 1,25(OH) 2 D 3 , 25(OH)D 3 uptake, K m , and V max returned to normal values. The effect of 25(OH)D 3 supplementation was also examined. In normal adults, 25(OH)D 3 administration had no effect on serum 1,25(OH) 2 D 3 levels nor on the K m or the V max of PBM 1α-hydroxylase but caused a 11-fold increase in serum 24R,25-dihydroxyvitamin D 3 [24R,25(OH) 2 D 3 ]. In HP, 25(OH)D 3 therapy raised serum 1,25(OH) 2 D 3 and reduced the K m and V max of PBM 1α-hydroxylase, which correlated negatively with serum 1,25(OH) 2 D 3 . However, serum 24R,25(OH) 2 D 3 only increased slightly above basal. These results demonstrate that, in HP, 1) impaired uptake of 25(OH)D 3 and low affinity for substrate determine the need for high 25(OH)D 3 levels to normalize serum 1,25(OH) 2 D 3 , despite higher enzymatic activity; 2) 1,25(OH) 2 D 3 deficiency plays a role in enhanced 1,25(OH) 2 D 3 synthesis and impaired access of 25(OH)D 3 to PBM 1α-hydroxylase; and 3) abnormal 25(OH)D 3 delivery also affects 24-hydroxylation
Spill code minimization techniques for optimizing compliers (1989)

Global register allocation and spilling is commonly performed by solving a graph coloring problem. In this paper we present a new coherent set of heuristic methods for reducing the amount of spill code generated. This results in more efficient (and shorter) compiled code. Our approach has been compared to both standard and priority-based coloring algorithms, universally outperforming them. In our approach, we extend the capability of the existing algorithms in several ways. First, we use multiple heuristic functions to increase the likelihood that less spill code will be inserted. We have found three complementary heuristic functions which together appear to span a large proportion of good spill decisions. Second, we use a specially tuned greedy heuristic for determining the order of deleting (and hence coloring) the unconstrained vertices. Third, we have developed a “cleaning” technique which avoids some of the insertion of spill code in non-busy regions.
Extremely low-noise potentiometry with a scanning tunneling microscope (1989)

Novel ac biasing and detection techniques have been developed to allow a scanning tunneling microscope (STM) to measure spatial variations in electric potential on metallic surfaces with sub-μV sensitivity. When implemented with a room-temperature STM operating with minimal electrical shielding and no vibration isolation, the voltage sensitivity was limited by the thermal (Johnson) noise of the tunneling resistance.
Competitive inhibition by lithium and hydrogen ions of the effect of calcium on the aggregation of rabbit platelets. (1976)

ADP-induced platelet aggregation and shape change were monitored optically in citrated rabbit platelet-rich plasma (PRP) diluted with isotonic salt solutions. Lithium (Li) produced a concentration-dependent reduction in the rate of platelet aggregation but had no discernible effect on the shape change which precedes aggregation. When PRP was pre-incubated with Li, the inhibitory effect of the ion was independent of the duration and temperature of the treatment. The inhibitory effect of Li also was observed in heparinized PRP or when 5-HT was used as the aggregation-inducing agent. When Li was combined with aggregation inhibitors which enhance platelet cyclic AMP content either by activating adenylate cyclase or by inhibiting phosphodiesterase, only additive effects were observed. The inhibitory effect of Li was opposed by added calcium. Kinetic evaluation of the interaction between Li and Ca indicated that their antagonism was competitive. Added calcium also displayed competitive antagonism toward the aggregation inhibiting effect of increased hydrogen ion concentration in the pH range between 6 and 8.
Analog circuit testing based on sensitivity computation and new circuit modeling (2002)

Analog circuit testing is considered to be a very difficult task, due mainly to the lack of fault models and accessibility to internal nodes. An approach is presented for analog circuit modeling and testing to overcome this problem. This circuit modeling is based on a sensitivity computation and on circuit structure, which are crucial in analog circuit testing. The testability of the circuit is achieved for the simple fault model and by functional testing. Component deviations are deduced by measuring a number of output parameters, and through sensitivity analysis and tolerance computation. Using this approach, adequate tests are identified for testing both catastrophic and soft faults. Some experimental results are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
PREPARATION OF STAR-SHAPED POLYMER WITH STEREOREGULAR POLY(METHYL METHACRYLATE) ARMS (2002)

ABSTRACT Isotactic (it-) PMMA anions prepared with t-C4H9MgBr in toluene were reacted with ethylene glycol dimethacrylate (EGDMA) in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) to obtain a star shaped polymer. 1H NMR, SEC-LALLS and SEC-differential viscometric analyses of the polymer clearly indicated that the polymer is a highly branched star polymer with crosslinked dimethacrylate core; the number of arms was estimated to be 20–30. A similar reaction of syndiotactic (st-) PMMA anions formed with t-C4H9Li-(n-C4H9)3Al in toluene with EGDMA was unsuccessful. However, the use of butane-1,4-diol dimethacrylate instead of EGDMA resulted in the effective formation of star polymers having st-PMMA arms; the number of arms was estimated to be 139. As a result, two types of star -shaped polymethacrylates with it- and st-PMMA arms were obtained.
Thrombin specificity with tripeptide chromogenic substrates: comparison of human and bovine thrombins with and without fibrinogen clotting activities. (1986)

To assess the thrombin specificity of tripeptide chromogenic substrates, we determined the Michaelis--Menten (Km), catalytic (kcat), and specificity (kcat/Km) constants for S-2238 (H-D-Phe-Pip-Arg-p-nitroanilide), Chromozym-TH (Tos-Gly-Pro-Arg-p-nitroanilide), and Spectrozyme-TH (H-D-hexahydrotyrosyl-Ala-Arg-p-nitroanilide) with high-purity thrombin preparations. Human and bovine alpha-thrombins, prepared by essentially the same procedure, were each greater than 95% in the form of the enzyme (alpha-thrombin) and had a specific fibrinogen-clotting activity greater than 2000 kilo-clotting units per gram of protein (kcu/g). In contrast, human gamma- and bovine beta-thrombins, made by controlled passage of alpha-thrombin through trypsin agarose, were greater than 98% of their respective forms and had fibrinogen-clotting activity less than 1 kcu/g. With the tripeptide chromogenic substrates these four thrombin forms at pH 7.8 and 23 degrees C had Km values of 1.6 to 16 mol/L, kcat values of 35 to 130 s-1, and kcat/Km ratios of 4.7 to 52 L X mol-1 X s-1. Although Km for an individual substrate was slightly higher for bovine than for human alpha-thrombins and although Km values for the nonclotting forms (human gamma- and bovine beta-thrombins) were higher than for clotting forms (alpha-thrombins), we found no major differences among the kinetic values for the three substrates.
Hypertrophic Chondrocytes (1990)

Chondrocytes emerging in the limb or other locations during embryogenesis are currently considered terminally differentiated cells and thus represent the last stage of differentiation in the chondrogenic cell lineage. Most chondrocytes, however, undergo further major phenotypic changes during late embryogenesis and early postnatal life as they take part in the endochondral ossification process. During this process, "resting" chondrocytes first enter an active, proliferative phase and then develop into large, round hypertrophic chondrocytes with unique phenotypic traits. The question thus arises as to whether hypertrophic chondrocytes actually represent the terminal stage of differentiation in the chondrogenic lineage. To assess the developmental position of these cells along the lineage, we examined the expression of four genes encoding extracellular matrix components in chondrocytes undergoing endochondral ossification in chicken tibial growth cartilage. We found that the steady-state levels of mRNAs coding for proteoglycan core protein increased in regions of cartilage destined for endochondral ossification. Similarly, type II collagen gene expression increased markedly in proliferating chondrocytes and then returned to basal levels in hypertrophic chondrocytes. As revealed by in situ hybridization, type X collagen gene expression was undetectable in resting and early proliferating chondrocytes and was detectable in hypertrophic chondrocytes. Osteonectin synthesis appeared to characterize chondrocytes in the resting, proliferating, and hypertrophic zones of growth cartilage. The protein was scarce, however, and cell-associated in the former zones, although it was very abundant and matrix-associated in the hypertrophic zone. Clearly, the emergence of hypertrophic chondrocytes during endochondral ossification is accompanied by marked quantitative and qualitative changes in gene expression. Interestingly, these changes occur during or immediately after the period of active chondrocyte proliferation. On the premises of the cell lineage definition proposed by Holtzer, the above data suggest that the hypertrophic chondrocytes represent the terminal stage of differentiation in the chondrogenic cell lineage.
Protein tyrosine phosphorylation in Mycobacterium tuberculosis (1994)

Crude cell extracts from three strains of Mycobacterium tuberculosis were analyzed for the presence of proteins possessing phosphorylated tyrosine residues. A protein migrating at approximately 55 kDa was detected using an antiphosphotyrosine monoclonal antibody. In addition, less predominant bands were observed between 50 kDa and 60 kDa. That M. tuberculosis contains specific tyrosine phosphorylated proteins implies that M. tuberculosis has tyrosine kinase activity. Examination of other, non-pathogenic mycobacterium species yielded no major antiphosphotyrosine reactive proteins. This suggests that the antiphosphotyrosine reactive protein is specific to M. tuberculosis strains. These results provide evidence that M. tuberculosis contains an antiphosphotyrosine reactive protein.
PERIPHERAL VASCULAR HEMODYNAMICS IN THE BULBAR CONJUNCTIVA OF SUBJECTS WITH HYPERTENSIVE VASCULAR DISEASE 123 (1951)

Within recent years, methods have been devel-oped for direct observational studies concerned with functional aspects of the muscular vessels in the capillary bed of living small mammals of sev-eral species (1-3). With these technics, it has been found that certain objective criteria of periph-eiral vascular reactivity can be selected and fol-lowed during repeated examinations of the termi-nal vessels with the microscope.- Comparisons of the several different overall hemodynamic disturb-ances in the experimental animal (i.e., hyperten-sion, acute hemorrhage, prolonged hypotension) with their co-existent phenomena in the peripheral vascular system have shown that each separate condition thus far investigated has had a distinc-tive status reflected in the capillary bed of the mesentery. For example, acute hemorrhage was accompanied by widespread arteriolar and metar-teriolar constriction, capillary ischemia, and poor venular return. The reactivity of metarterioles and precapillary sphincters to topically applied epinephrine was augmented. Terminal prolonged hemorrhagic or traumatic hypotension, on the other hand, had a dilated capillary bed, with slug-gish arteriolar and venular blood flow, plethoric capillaries and diminished epinephrine responses (2, 4, 5). The mesenteric vascular bed in experi-mental renal hypertension was predominantly con-stricted, with ischemic arterioles and capillaries and a similar elevation of epinephrine response. In addition, it had a marked &quot;hyperplasia &quot; of vascu-lar elements (6). 1This work was supported in part by the Nutrition Foundation, New York, N. Y. 2Apparatus used for the study of the conjunctiva was obtained through a grant in aid from the American Philo-
Electrooptic radiation modulators utilizing semiconductor ridged waveguides (1980)

Investigations of phase-polarization and amplitude modulation of radiation are reported. Effective control voltages of ~10 V were obtained for a modulation depth of ~70%. A radiation deflector utilizing an array of channel waveguides was investigated.
Corrosion Potentials and Corrosion Behavior of AISI 304 Stainless Steel in High-Temperature Water Containing Both Dissolved Hydrogen and Oxygen (1986)

The corrosion potential of AISI 304 stainless steel (SS) in high-temperature water is affected markedly by the absolute and relative concentrations of dissolved hydrogen and oxygen as well as by mass transport of these species to the metal surface. This reflects the coupled electrochemical recombination reaction of the hydrogen and oxygen on the surface. The fact that the effects are more pronounced in water containing a stoichiometric excess of hydrogen than in the reverse case suggests that the electrochemical oxidation of hydrogen on an oxide-covered steel electrode is markedly inhibited. The work also implies that when hydrogen injection is used to control the corrosion potentials of SS, monitoring and maintaining low oxygen concentrations in the circulating cooling water is more important than maintaining a specific hydrogen level if the latter is in stoichiometric excess of the oxygen.
Varicose Veins, Oral Contraceptives, and Thromboembolism (1967)

the villages there was no significant change in the incidence of scabies.About a year ago we stopped using benzyl benzoate and started using monosulfiram soap, advising the patients to allow the soap to dry on the skin.In this way an effective concentration of monosulfiram is built up on the skin and in the clothes and bedding.The results have been quite remarkable, and scabies has almost disappeared in some villages.The only obstacle to complete eradication seems to be the cost of the soap at 2s. each in a country where a manual worker earns only 6s. a day when he can find work.I was surprised to see no mention of mono- sulfiram in the copies of the B.M.7.which have reached me so far.-Iam, etc.,
Capacity Constraints and Cost-Effectiveness: A Discrete Event Simulation for Drug-Eluting Stents (2009)

Our model shows that neglected limited capacities can cause wrong cost-effectiveness results. Therefore, capacities should be explicitly included in decision-analytic models if there is evidence of scarcity.
Total Synthesis of (+)-13-Deoxytedanolide (2005)

A total synthesis of 13-deoxytedanolide is described. The synthesis features a highly stereoselective fragment assembly aldol reaction of methyl ketone 4 and aldehyde 5 to establish the complete carbon skeleton of the natural product in the form of aldol 15. The facile formation of the remarkably unreactive hemiketal 16 thwarted attempts to elaborate 15 to tedanolide. However, deoxygenation of the C(13)-hydroxyl of 16 provided the 13-deoxy hemiketal 17 that was smoothly elaborated to 13-deoxytedanolide.
Electrosynthesized NiS2 thin films and their optical and semiconductor studies (2013)

Abstract: Nickel sulfide thin films were prepared using electrodeposition on indium tin oxide-coated glass substrates. Films were characterized using X-ray diffraction for crystallographic analysis. The films were shown to be polycrystalline in nature with good uniformity. From scanning electron micrographs, the surface appeared to be comparatively granular with irregularly shaped grains. From optical analysis, the bandgap range was between 1.22 eV and 1.15 eV with an indirect bandgap nature. Mott-Schottky plot confirmed that the films were found to be n-type, and the semiconductor parameters of the film were derived. Keywords: X-ray diffraction, scanning electron micrograph, optical studies, Mott-Schottky analysis
IL15 Enhances CAR-T Cell Antitumor Activity by Reducing mTORC1 Activity and Preserving Their Stem Cell Memory Phenotype (2019)

Improvements in the quality and fitness of chimeric antigen receptor (CAR)-engineered T cells, through CAR design or manufacturing optimizations, could enhance the therapeutic potential of CAR-T cells. One parameter influencing the effectiveness of CAR-T cell therapy is the differentiation status of the final product: CAR-T cells that are less-differentiated and less exhausted are more therapeutically effective. In the current study, we demonstrate that CAR-T cells expanded in IL15 (CAR-T/IL15) preserve a less-differentiated stem cell memory (Tscm) phenotype, defined by expression of CD62L<sup>+</sup>CD45RA<sup>+</sup> CCR7<sup>+</sup>, as compared with cells cultured in IL2 (CAR-T/IL2). CAR-T/IL15 cells exhibited reduced expression of exhaustion markers, higher antiapoptotic properties, and increased proliferative capacity upon antigen challenge. Furthermore, CAR-T/IL15 cells exhibited decreased mTORC1 activity, reduced expression of glycolytic enzymes and improved mitochondrial fitness. CAR-T/IL2 cells cultured in rapamycin (mTORC1 inhibitor) shared phenotypic features with CAR-T/IL15 cells, suggesting that IL15-mediated reduction of mTORC1 activity is responsible for preserving the Tscm phenotype. CAR-T/IL15 cells promoted superior antitumor responses <i>in vivo</i> in comparison with CAR-T/IL2 cells. Inclusion of cytokines IL7 and/or IL21 in addition to IL15 reduced the beneficial effects of IL15 on CAR-T phenotype and antitumor potency. Our findings show that IL15 preserves the CAR-T cell Tscm phenotype and improves their metabolic fitness, which results in superior <i>in vivo</i> antitumor activity, thus opening an avenue that may improve future adoptive T-cell therapies.