gebhart/patrepeval huggingface dataset:
Construction of an Acetylcholinesterase Sensor Based on Synthesized Paramagnetic Nanoparticles, a Simple Tool for Neurotoxic Compounds Assay (2017)

Magnetic particles (MPs) have been widely used in biological applications in recent years as a carrier for various molecules. Their big advantage is in repeated use of immobilized molecules including enzymes. Acetylcholinesterase (AChE) is an enzyme playing crucial role in neurotransmission and the enzyme is targeted by various molecules like Alzheimer's drugs, pesticides and warfare agents. In this work, an electrochemical biosensor having AChE immobilized onto MPs and stabilized through glutaraldehyde (GA) molecule was proposed for assay of the neurotoxic compounds. The prepared nanoparticles were modified by pure AChE and they were used for the measurement anti-Alzheimer's drug galantamine and carbamate pesticide carbofuran with limit of detection 1.5 µM and 20 nM, respectively. All measurements were carried out using screen-printed sensor with carbon working, silver reference, and carbon auxiliary electrode. Standard Ellman's assay was used for validation measurement of both inhibitors. Part of this work was the elimination of reversible inhibitors represented by galantamine from the active site of AChE. For this purpose, we used a lower pH to get the original activity of AChE after inhibition by galantamine. We also observed decarbamylation of the AChE-carbofuran adduct. Influence of organic solvents to AChE as well as repeatability of measurement with MPs with AChE was also established.
SVC CE1: STool - a native spatially scalable approach to SVC (2004)

This documents describes the UNIBS-SCL proposal in response to the MPEG21 SVC CE1 [1]. Our scalable video coding scheme, called STool, is based on a 2D+t+2D structure and is implemented using a modified version of the Microsoft Research Asia (MSRA) reference software [2] plus some modifications and tools which has been used in substitution. The STool architecture has been implemented in two different systems. In System-1 the modules provided in the MSRA software have been used to build the new STool architecture. In System-2 we test a new entropy coder, called GOF-EMDC, which is an extended version of the EMDC coder [3]. At the time GOF-EMDC codec and other parts of System-2 have not been optimized in many aspects, therefore we can expect better performance from our system in the next future. Despite this fact System-2 provides similar coding performances when compared to System-1. In addition, System-2 is much more flexible in many aspects, it guarantees a major number of functionalities and better fulfill the requirements list. Therefore with System-1 we intend to demonstrate the characteristics of the STool architecture, especially with respect to the reference software used, while with System-2 we customize and add functionalities to Stool. We submitted extraction and decoding software for both Systems-1 and System-2, System-1 coded sequences for both scenarios 1 and 2 and System-2 coded sequences for scenario 2 only. For System-2 scenario 1 we only had deadline problems. No technical problems actually exist to produce such sequences.
PEO‐Like Polymer Electrolytes with High Room Temperature Conductivity (1997)

Polymer electrolytes with room temperature conductivities of S/cm were prepared by encapsulating solutions of (or or ) in oligomeric poly(ethylene glycol) dimethyl ethers in a poly(vinylidene fluoride)‐hexafluoropropene copolymer. The 100 to 200 μm thick membranes prepared have good mechanical strength and are "dry" with practically no volatility at least up to 70°C. Because of these properties and the structural similarities between poly(ethylene glycol) dimethyl ethers (PEGDME) and polyethylene oxide (PEO) we call them PEO‐like.
Oblivious Evaluation of Non-deterministic Finite Automata with Application to Privacy-Preserving Virus Genome Detection (2014)

Various string matching problems can be solved by means of a deterministic finite automaton (DFA) or a non-deterministic finite automaton (NFA). In non-oblivious cases, DFAs are often preferred for their run-time efficiency despite larger sizes. In oblivious cases, however, the inevitable computation and communication costs associated with the automaton size are more favorable to NFAs. We propose oblivious protocols for NFA evaluation based on homomorphic encryption and demonstrate that our method can be orders of magnitude faster than DFA-based methods, making it applicable to real-life scenarios, such as privacy-preserving detection of viral infection using genomic data.
Control of human papillomavirus type 11 origin of replication by the E2 family of transcription regulatory proteins (1992)

Replication of human papillomavirus type 11 (HPV-11) DNA requires the full-length viral E1 and E2 proteins (C.-M. Chiang, M. Ustav, A. Stenlund, T. F. Ho, T. R. Broker, and L. T. Chow, Proc. Natl. Acad. Sci. USA 89:5799-5803, 1992). Using transient transfection of subgenomic HPV DNA into hamster CHO and human 293 cells, we have localized an origin of replication (ori) to an 80-bp segment in the upstream regulatory region spanning nucleotide 1. It overlaps the E6 promoter region and contains a short A + T-rich segment and a sequence which is homologous to the binding site of the bovine papillomavirus type 1 (BPV-1) E1 protein in the BPV-1 ori. However, unlike the BPV-1 ori, for which half an E2-responsive sequence (E2-RS) or binding site suffices, an intact binding site is essential for the HPV-11 ori. Replication was more efficient when additional E2-RSs were present. The intact HPV-11 genome also replicated in both cell lines when supplied with E1 and E2 proteins. Expression vectors of transcription repressor proteins that lack the N-terminal domain essential for E2 transcriptional trans activation did not support replication in collaboration with the E1 expression vector. Rather, cotransfection with the repressor expression vectors inhibited ori replication by the E1 and E2 proteins. These results demonstrate the importance of the N-terminal domain of the E2 protein in DNA replication and indicate that the family of E2 proteins positively and negatively regulates both viral DNA replication and E6 promoter transcription.
Effect of T-cell depletion as graft-versus-host disease prophylaxis on engraftment, relapse, and disease-free survival in unrelated marrow transplantation for chronic myelogenous leukemia (1994)

Between January 1988 and March 1993, 48 patients received T-cell-depleted marrow grafts from unrelated donors as treatment for chronic myelogenous leukemia (CML). The median age of the population was 31.7 years (range 5.4 to 53) with 17 of 48 patients greater than 40 years of age. Twenty-seven patients were transplanted in chronic phase, 17 in accelerated phase, and 4 in blast crisis. All patients received a standardized preparative regimen of cyclophosphamide, high-dose cytosine arabinoside, methylprednisolone, and total body irradiation. Marrow grafts were depleted of mature T cells with the alpha beta T-cell receptor antibody T10B9 as graft-versus-host disease (GVHD) prophylaxis. All patients also received posttransplant cyclosporine therapy. Twenty-eight of 48 patients were mismatched with their donors for one or more HLA-A, B, DR, or DQ loci by either serology or high-resolution oligonucleotide genotyping. Nine of 28 were mismatched at multiple HLA loci. Durable engraftment was achieved in 94% (45/48) of patients. The actuarial probability of developing grades II to IV and grades III to IV acute GVHD were 39.6% (95% confidence interval (CI) 26.9 to 53.0) and 8.3% (95% CI 6.1 to 10.9) for the entire cohort. There was no difference in the incidence of grades II to IV acute GVHD between patients receiving matched (36.8%) or mismatched (41.4%) marrow grafts (P = .77). The actuarial probability of relapse at 2 years was 8.8% (95% CI 2.1 to 21.6) for the entire cohort and 18% (95% CI 4 to 41) for patients transplanted in either the accelerated or blast crisis phase (advanced disease). One cytogenetic relapse has occurred among patients transplanted in the chronic phase. The probability of disease-free survival at 2 years was 52% (95% CI 24 to 70) for patients transplanted in chronic phase and 46% (95% CI 25 to 73) for patients transplanted with advanced disease. No difference in disease-free survival was observed between patients receiving matched (49%) or mismatched (51%) marrow grafts (P = .90). This study shows that patients receiving unrelated T-cell-depleted marrow grafts for CML can achieve durable engraftment with a low incidence of severe GVHD and apparent preservation of graft-versus-leukemia reactivity. These data also suggest that T-cell depletion may allow patients who might otherwise experience unacceptable toxicity from GVHD-related complications caused by older age or increased HLA disparity to benefit from unrelated marrow grafts.
MLD and mse algorithms for adaptive detection of digital signals in the presence of interchannel interference (1977)

Adaptive mean-square error (mse) and maximum-likelihood detection (MLD) algorithms for a dual-channel digital communication system in the presence of interchannel interference and white Gaussian noise are presented. The mse algorithm forms estimates of the transmitted symbols from a linear combination of received symbols using weights that minimize the mse between transmitted and estimated symbols. The nonlinear MLD algorithm minimizes the probability of symbol error by maximizing the probability of the received signal samples on the two channels over ail possible transmitted symbol pairs. The probability of error is derived for the two algorithms when quadrature phase-shift keying (QPSK) is used as a modulation technique, and is compared with that of a dual-channel QPSK system having no compensation for the crosstalk.
Successive Deposition of Alternate Layers of Polyelectrolytes and a Charged Virus (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSuccessive Deposition of Alternate Layers of Polyelectrolytes and a Charged VirusYuri Lvov, Heinrich Haas, Gero Decher, Helmuth Moehwald, Albert Mikhailov, Boris Mtchedlishvily, Ekaterina Morgunova, and Boris VainshteinCite this: Langmuir 1994, 10, 11, 4232–4236Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November 1994https://pubs.acs.org/doi/10.1021/la00023a052https://doi.org/10.1021/la00023a052research-articleACS PublicationsRequest reuse permissionsArticle Views657Altmetric-Citations282LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Growth Factors and Corneal Endothelial Cells: I. Stimulation of Bovine Corneal Endothelial Cell DNA Synthesis by Defined Growth Factors (1992)

Peptide growth factors and other physiological growth modifiers were evaluated for their ability to stimulate DNA synthesis in early passage cultures of bovine corneal endothelial cells (BCEC). Increasing concentrations of newborn bovine serum (0.5-10%) causes a progressive increase in DNA synthesis, which approached a plateau at 10% serum. Supplementing medium with 10% serum from different lots of newborn bovine serum or fetal bovine serum stimulated significantly different levels of DNA synthesis by BCEC. Addition of epidermal growth factor (EGF) (2 nM) to medium containing 10% newborn or fetal bovine serum further increased DNA synthesis. Dose-response curves for EGF, transforming growth factor-alpha, basic fibroblast growth factor (bFGF), and insulin-like growth factor I showed that each significantly stimulated high levels of DNA synthesis (200-700% increase) compared with BCEC cultured in serum-free medium. Vaccinia growth factor, insulin, and transforming growth factor-beta each significantly stimulated lower levels of DNA synthesis (30-200% increase), whereas nerve growth factor, multiplication stimulating activity, and platelet-derived growth factor all failed to significantly stimulate DNA synthesis above the level of serum-free medium. Other physiological growth modifiers were tested for their effects on DNA synthesis of BCEC. Transferrin and low levels of 3',5'-cyclic monophosphate (cAMP) stimulated very low levels of DNA synthesis (50% increase) whereas linoleic acid, high levels of selenium, or cAMP each inhibited DNA synthesis 25-75% below the level of BCEC cultured in serum-free medium. A series of eight formulations containing various combinations of EGF, FGF, insulin, transferrin, selenium, linoleic acid, retinoic acid, cAMP, heparin, and endothelial cell growth factor were tested for their mitogenic action on BCEC cultures. A formulation containing EGF, insulin, transferrin, selenium, and linoleic acid (EGF + ITSL) stimulated the highest level of DNA synthesis of BCEC, which was approximately 25% higher than the increase stimulated by addition of 10% newborn bovine serum. The formulation consisting of EGF + ITSL was also evaluated as a supplement to corneal storage media. Addition of EGF + ITSL to three corneal storage media (McCarey-Kaufman, K-Sol, CSM) significantly stimulated increases in cell numbers of approximately 50% above the unsupplemented corneal storage media. These results demonstrate that BCEC respond selectively to different defined peptide growth factors and physiological growth modifiers, and suggest that supplementation of corneal storage media with a defined formulation (EGF + ITSL) may enhance corneal endothelial cell density.
Human Factors in Flight - Second Edition (1993)

This is the Revised Second Edition of the pioneering book that helped the International Civil Aviation Organization (ICAO) to mastermind their Human Factors Digests, that enabled many to implement the Human Factors and Limitations regulations and syllabuses, and that has equipped thousands of students, instructors and experienced aviators with an in-depth knowledge of Human Factors technology. It has new material, sections and references.
Towards Software-Defined Massive MIMO for 5G&B Spectral-Efficient Networks (2018)

As a key enabling technology for 5G&B systems, massive multi-input multi-output (MIMO) allows the system capacity to be theoretically increased by simply installing additional antennas to remote radio heads (RRHs). However, this innovative technology cannot support higher data capacity without accurate channel state information and interference handling, especially for multi-cell scenarios. In this paper, the dynamic macro- diversity (i.e., network) massive MIMO is treated from the perspective of software-defined cellular architecture. The so-called software-defined massive MIMO is introduced, which dynamically coordinates highly-deployed RRHs equipped with massive antennas so that the maximum spectral efficiency is achieved. First, the software- defined cellular architecture is presented, where distributed massive antenna systems with centralized control and time-division duplexing massive MIMO are investigated. Next, in this considered architecture, a rigid analysis of achievable ergodic user sum-rates is given for macro-diversity massive MIMO schemes. An optimization framework of software-defined massive MIMO is further proposed that optimizes RRH clustering pattern and RRH-user associations while satisfying system-level constraints. To address the NP-complete problem of the optimal framework design, an iterative, global search algorithm is developed that exploits genetic algorithms and yields satisfactory solutions in only few rounds. Performance evaluation validates the efficacy of our solution which facilitates universal frequency reuse for 5G&B wireless networks.
The Applications of Low-cost Liquid Crystal Display for Light Field Modulation and Multimodal Microscopy Imaging (2015)

Liquid crystal display (LCD) technology has enjoyed significant advances in just a few short years. The quality of LCD panels has improved dramatically while at the same time costs have gradually come down. LCDs are now found in products as small as mobile phones and as large as 42-inch flat panel screens. The thesis will introduces and discusses two new applications of Liquid crystal display.\nAnti-headlamp digital glass: In recent years, public concerns regarding headlamp glare have substantially increased and become one of the most active dockets in National Highway Traffic Safety Administration. We report an innovative design of a digital wearable glass that can reduce the discomforting glare and improve human’s night vision.\nMicroscopy illumination engineering using a low-cost liquid crystal display: In a typical microscope, the condenser lens provides sample illumination that is uniform and free from glare. The associated condenser diaphragm can be manually adjusted to obtain the optimal illumination numerical aperture. We report a programmable condenser lens for active illumination control. In our prototype setup, we used a $15 liquid crystal display as a transparent spatial light modulator and placed it at the back focal plane of the condenser lens.
Composition of Milk Fat Fractions Obtained by Fractional Crystallization from Acetone (1966)

Milk fat was separated into seven fractions and a residue by fractional crystallization from acetone. A special device is described for the separation of fat cwstals at controlled temperatures in a thermostat bath. The fatty acid composition of the
Severe Enteric Disease in an Animal Shelter Associated with Dual Infections by Canine Adenovirus Type 1 and Canine Coronavirus (2001)

An outbreak of dual infection in dogs with canine adenovirus type 1 (CAV-1) and canine coronavirus (CCV) infection is reported in an animal shelter that comprised approximately 200 adults stray dogs and 30 puppies. Twenty puppies died 7-8 days after the onset of the clinical signs (severe enteritis, leucopoenia, respiratory distress and dehydration). Both CAV-1 and CCV were isolated from tissue or swab samples. Antibodies to CCV and, at high levels, to CAV-1 also were detected in several puppies. The principal histological findings were atrophy of small intestinal villi, lymphoid depletion, hepatitis and bronchopneumonia. The persistence of CCV in the faeces, observed by the polymerase chain reaction assay, was longer than previously reported. Results demonstrated the serious consequences which may occur with dual infections by CAV-1 and CCV in assembled groups of dogs that are housed in poorly managed kennels with inadequate vaccination programmes.
Fabrication of NiO Microflake@NiFe-LDH Nanosheet Heterostructure Electrocatalysts for Oxygen Evolution Reaction (2018)

The development of highly efficient eletrocatalysts for water electrolysis application is a rising hot issue; especially the oxygen evolution reaction (OER) attracted wide attention due to the significant overpential derived by the complex four electron-transfer coupled reaction. In this study, hierarchical NiO microflake@NiFe-layer double hydroxide (LDH) nanosheet core–shell arrays on nickel foam were fabricated by combining a two-step hydrothermal synthesis and a calcination process. Due to the three-dimensional rational configuration, the obtained composite electrode showed high performance for OER, which needed a low apparent overpotential of 265 mV to afford a 10 mA cm–2 current density. In addition, the NiO@NiFe-LDH coated electrode was further treated in an organic solution via ultrasound for in situ intercalation of the NiFe-LDH to provide wide channels for ion transportation and gas diffusion. As a result, the charge transition resistance was obviously reduced and the apparent activity as well as the long-term stability was improved. The optimized NiO@NiFe-LDH composite exhibited an excellent long-term stability in 50 h even at high current densities of 50 and 100 mA cm–2, and meanwhile the required apparent overpotential of OER for sustaining 10 mA cm–2 current density was reduced from 265 to 210 mV.
The effect of temperature on the induction time of a stabilized oil (1991)

Soybean oil was fortified with the antioxidants BHT (butylated hydroxytoluene), BHA (butylated hydroxyanisole), TBHQ (tertiary butylhydroquinone), rosemary extract (Herbalox® Seasoning) and tocopherol. Induction times were determined against a control on each sample in a Metrohm Rancimat over a temperature range of 80°C to 180°C. A linear effect of the data was obtained when the log of induction time was plotted against temperature. The Metrohm Rancimat was found to be capable of determining induction times within the range of 0.5 to 70 hr.
Detection of human papillomavirus in sanitary napkins: A new paradigm in cervical cancer screening (2003)

Human papillomavirus was successfully detected by polymerase chain reaction (PCR) in menstrual blood or vaginal discharge collected in sanitary napkins in 100% of 17 women having koilocytosis, cervical intraepithelial neoplasia, or squamous carcinoma. We advocate this form of cervical cancer screening because of its high sensitivity and acceptance by patients.
Polyacrylamide Gel Identification of Bacterial L-Forms and <i>Mycoplasma</i> Species of Human Origin (1971)

Polyacrylamide gel electrophoretic patterns of acidified phenol extracts prepared from whole cells can be used for the identification of bacterial L-forms and Mycoplasma species of human origin. Ten human Mycoplasma serotypes and eight L-forms belonging to five different genera were studied. The gel patterns were sufficiently distinct and reproducible that it was possible not only to identify L-forms at the genus level (group with streptococci) and different Mycoplasma serotypes but also to differentiate between the two of them. The parentage of L-forms of Streptobacillus moniliformis L1, Listeria monocytogenes, Streptococcus MG, and Staphylococcus aureus Smith strain was established by relating their gel patterns directly to parent bacteria. It was found that an L-form designated S. moniliformis An (ATCC 14220) was actually an L-form of Proteus. In addition, it was shown electrophoretically that no relationship existed between the Streptococcus MG L-form and M. pneumoniae. The applicability of this method as a diagnostic and taxonomic tool for the differentiation of L-forms and mycoplasmas is discussed.
FLC microdisplays (2000)

Abstract Microdisplays made from ferroelectric liquid crystals (FLCs) and silicon integrated circuits offer exceptionally high resolutions along with the capability for single-panel sequential color. The binary pixels of these microdisplays are capable of full gray scale through the use of techniques that involve illumination modulation, subpixelation, and temporal dither. Continuous, DC-balanced display, even with the polarity-sensitive response of the FLC, can be achieved by using an electro-optic contrast inverter; alternately, image sticking can be minimized by specialized overdrive techniques that periodically toggle all the pixels.