gebhart/patrepeval huggingface dataset:
Influence of Post-Traumatic Stress Disorder on Neuroinflammation and Cell Proliferation in a Rat Model of Traumatic Brain Injury (2013)

Long-term consequences of traumatic brain injury (TBI) are closely associated with the development of severe psychiatric disorders, such as post-traumatic stress disorder (PTSD), yet preclinical studies on pathological changes after combined TBI with PTSD are lacking. In the present in vivo study, we assessed chronic neuroinflammation, neuronal cell loss, cell proliferation and neuronal differentiation in specific brain regions of adult Sprague-Dawley male rats following controlled cortical impact model of moderate TBI with or without exposure to PTSD. Eight weeks post-TBI, stereology-based histological analyses revealed no significant differences between sham and PTSD alone treatment across all brain regions examined, whereas significant exacerbation of OX6-positive activated microglial cells in the striatum, thalamus, and cerebral peduncle, but not cerebellum, in animals that received TBI alone and combined TBI-PTSD compared with PTSD alone and sham treatment. Additional immunohistochemical results revealed a significant loss of CA3 pyramidal neurons in the hippocampus of TBI alone and TBI-PTSD compared to PTSD alone and sham treatment. Further examination of neurogenic niches revealed a significant downregulation of Ki67-positive proliferating cells, but not DCX-positive neuronally migrating cells in the neurogenic subgranular zone and subventricular zone for both TBI alone and TBI-PTSD compared to PTSD alone and sham treatment. Comparisons of levels of neuroinflammation and neurogenesis between TBI alone and TBI+PTSD revealed that PTSD did not exacerbate the neuropathological hallmarks of TBI. These results indicate a progressive deterioration of the TBI brain, which, under the conditions of the present approach, was not intensified by PTSD, at least within our time window and within the examined areas of the brain. Although the PTSD manipulation employed here did not exacerbate the pathological effects of TBI, the observed long-term inflammation and suppressed cell proliferation may evolve into more severe neurodegenerative diseases and psychiatric disorders currently being recognized in traumatized TBI patients.
Regulation of gene expression in oncogenically transformed cells (1992)

Several genes expressed in response to growth factors are also regulated aberrantly in oncogenically transformed cells. The constitutive expression of genes encoding extracellular proteases, transcription factors, and cytokines is often correlated with cell transformation. In several instances, the uncontrolled expression of these genes is the result of transcriptional activation. Therefore, much attention has been devoted to the study of promoter function in transformed cells. We now review the results of recent investigations on transformation-dependent gene expression. The activation of several transcription factors in oncogenically-transformed cells is described. Results regarding the regulation of promoters through PRD II/kappa B are presented for cells transformed by a variety of oncogenes. Finally, we discuss the significance of transcription factor activation in the process of cell transformation.
The impact of frequency-flat fading on the spectral efficiency of CDMA (2001)

The capacity of the randomly spread synchronous code-division multiple-access (CDMA) channel subject to frequency-flat fading is studied in the wide-band limit of large number of users. We find the spectral efficiency as a function of the number of users per chip, the distribution of the flat fading, and the signal-to-noise ratio (SNR), for the optimum receiver as well as linear receivers (single-user matched filter, decorrelator, and minimum mean-square error (MMSE)). The potential improvements due to both decentralized transmitter power control and multi-antenna receivers are also analyzed.
Driver hand activity analysis in naturalistic driving studies: challenges, algorithms, and experimental studies (2013)

We focus on vision-based hand activity analysis in the vehicular domain. The study is motivated by the overarching goal of understanding driver behavior, in particular as it relates to attentiveness and risk. First, the unique advantages and challenges for a nonintrusive, vision-based solution are reviewed. Next, two approaches for hand activity analysis, one relying on static (appearance only) cues and another on dynamic (motion) cues, are compared. The motion-cue-based hand detection uses temporally accumulated edges in order to maintain the most reliable and relevant motion information. The accumulated image is fitted with ellipses in order to produce the location of the hands. The method is used to identify three hand activity classes: (1) two hands on the wheel, (2) hand on the instrument panel, (3) hand on the gear shift. The static-cue-based method extracts features in each frame in order to learn a hand presence model for each of the three regions. A second-stage classifier (linear support vector machine) produces the final activity classification. Experimental evaluation with different users and environmental variations under real-world driving shows the promise of applying the proposed systems for both postanalysis of captured driving data as well as for real-time driver assistance.
Circular RNAs and Their Emerging Roles in Immune Regulation (2018)

Circular ribonucleic acid (RNA) molecules (circRNAs) are covalently closed loop RNA molecules with no 5' end caps or 3' poly (A) tails, which are generated by back-splicing. Originally, circRNAs were considered to be byproducts of aberrant splicing. However, in recent years, development of high-throughput sequencing has led to gradual recognition of functional circRNAs, and increasing numbers of studies have elucidated their roles in cancer, neurologic diseases, and cardiovascular disorders. Nevertheless, studies of the functions of circRNAs in the immune system are relatively scarce. In this review, we detail relevant research on the biogenesis and classification of circRNAs, describe their functional mechanisms and approaches to their investigation, and summarize recent studies of circRNA function in the immune system.
PD-1/PD-L pathway and autoimmunity (2005)

Programmed cell death 1 (PD-1) was isolated in 1992 by subtractive-hybridization technique, as a molecule whose expression is enhanced by apoptotic stimuli. Since then we have been analyzing the function of PD-1 in the regulation of immune responses. Generation of PD-1 deficient mice, pathophysiological analyses of autoimmune diseases in PD-1 deficient mice, identification of two ligands, and analyses of downstream events of PD-1 revealed that PD-1 prevents autoimmunity by inhibiting activation of self-reactive lymphocytes. These findings were further applied on human autoimmune diseases and single nucleotide polymorphisms (SNPs) on human PD-1 gene have been reported to link with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and type I diabetes.
Reconfigurable magnetologic computing using MRAM cells (2007)

A simple hardware concept for a programmable logic element based on a single magnetic random access memory (MRAM) cell is presented. It combines the inherent advantage of a non-volatile output with flexible functionality which has to be selected at run-time. A single MRAM cell can represent one out of up to ten logic functions, among them the four elementary functions AND, OR, NAND and NOR and the two more complex functions XOR and XNOR if bipolar currents are used. Based on recent developments of the MRAM technology, which uses the toggled switching mode, alternative concepts for real MRAM devices will be discussed. As a first application example, which takes advantage of the reprogrammability and non-volatility of the logic elements, a compact design of a magnetologic full adder is presented.
Flowfield Measurements for Film-Cooling Holes With Expanded Exits (1998)

One viable option to improve cooling methods used for gas turbine blades is to optimize the geometry of the film-cooling hole. To optimize that geometry, effects of the hole geometry on the complex jet-in-crossflow interaction need to be understood. This paper presents a comparison of detailed flowfield measurements for three different single, scaled-up hole geometries, all at a blowing ratio and density ratio of unity. The hole geometries include a round hole, a hole with a laterally expanded exit, and a hole with a forward-laterally expanded exit. In addition to the flowfield measurements for expanded cooling hole geometries being unique to the literature, the testing facility used for these measurements was also unique in that both the external mainstream Mach number (Ma∞ = 0.25) and internal coolant supply Mach number (Mac = 0.3) were nearly matched. Results show that by expanding the exit of the cooling holes, both the penetration of the cooling jet and the intense shear regions are significantly reduced relative to a round hole. Although the peak turbulence level for all three hole geometries was nominally the same, the source of that turbulence was different. The peak turbulence level for both expanded holes was located at the exit of the cooling hole resulting from the expansion angle being too large. The peak turbulence level for the round hole was located downstream of the hole exit where the velocity gradients were very large.
Isolation and characterization of two new homoacetogenic hydrogen-utilizing bacteria from the human intestinal tract that are closely related to Clostridium coccoides (1997)

Two gram-positive, strictly anoxic, coccoid- to rod-shaped strains of bacteria, Clostridium coccoides 1410 and C. coccoides 3110, were isolated from human feces on the typical homoacetogenic substrates formate plus H2 plus CO2 (strain 1410) and vanillate plus H2 plus CO2 (strain 3110) in the presence of 2-bromoethanesulfonate to inhibit methanogenesis. On the basis of 16S rRNA sequencing, DNA-DNA hybridization, and physiological and morphological parameters, both isolates are closely related to C. coccoides DSM 935T. The G+C contents of the DNA were 46.1 and 46.2 mol% for C. coccoides 1410 and C. coccoides 3110, respectively. Cytochromes could not be detected. Formate was degraded exclusively to acetate, whereas vanillate was O-demethylated, resulting in acetate and 3,4-dihydroxybenzoate, the latter being further decarboxylated to catechol. In the presence of organic substrates, H2 was cometabolized to acetate, but both strains failed to grow autotrophically. Lactose, lactulose, sorbitol, glucose, and various other carbohydrates supported growth as well. Untypical of homoacetogens, glucose and sorbitol were fermented not exclusively to acetate; instead, considerable amounts of succinate and D-lactate were produced. H2 was evolved from carbohydrates only in negligible traces. Acetogenesis from formate plus H2 plus CO2 or vanillate plus H2 plus CO2 was constitutive, whereas utilization of carbohydrates was inducible. Hydrogenase, CO dehydrogenase, formate dehydrogenase, and all of the tetrahydrofolic acid-dependent, C1 compound-converting enzymes of the acetyl-coenzyme A pathway of homoacetogenesis were present in cell extracts.
High power CW Tm:YLF laser with a holographic output coupler (2004)

We report a diode-pumped, CW Tm:YLF laser, with a volume holographic grating in photo-thermo-refractive glass as an output coupler. The Tm-laser operated at 1940 nm with output power exceeding 30 W.
Anti‐β<sub>2</sub>M monoclonal antibodies kill myeloma cells <i>via</i> cell‐ and complement‐mediated cytotoxicity (2014)

Our previous studies showed that anti-β2M monoclonal antibodies (mAbs) at high doses have direct apoptotic effects on myeloma cells, suggesting that anti-β2M mAbs might be developed as a novel therapeutic agent. In this study, we investigated the ability of the mAbs at much lower concentrations to indirectly kill myeloma cells by utilizing immune effector cells or molecules. Our results showed that anti-β2M mAbs effectively lysed MM cells via antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), which were correlated with and dependent on the surface expression of β2M on MM cells. The presence of MM bone marrow stromal cells or addition of IL-6 did not attenuate anti-β2M mAb-induced ADCC and CDC activities against MM cells. Furthermore, anti-β2M mAbs only showed limited cytotoxicity toward normal B cells and nontumorous mesenchymal stem cells, indicating that the ADCC and CDC activities of the anti-β2M mAbs were more prone to the tumor cells. Lenalidomide potentiated in vitro ADCC activity against MM cells and in vivo tumor inhibition capacity induced by the anti-β2M mAbs by enhancing the activity of NK cells. These results support clinical development of anti-β2M mAbs, both as a monotherapy and in combination with lenalidomide, to improve MM patient outcome.
Factors Influencing Delayed Gastric Emptying After Pylorus-Preserving Pancreatoduodenectomy (2003)

Preoperative cholangitis and postoperative leakage problems affect gastric emptying after PPPD during the early postoperative period. The retromesenteric route is not recommended for pancreato- and biliary-enteric anastomosis. We speculate that retromesenteric passage of afferent jejunum can cause venous congestion and bowel edema, which can in turn retard the recovery of jejunal peristalsis at the duodenojejunostomy site resulting in disturbance from food passage.
Chlorofluorocarbons in medicinals (1990)

Journal Article Chlorofluorocarbons in medicinals Get access Philip O. Anderson, Pharm D., FASHP Philip O. Anderson, Pharm D., FASHP Director Drug Information Service, Department of Pharmacy, University of California San Diego Medical Center, 225 Dickinson Street, San Diego, CA 92103 Search for other works by this author on: Oxford Academic Google Scholar American Journal of Hospital Pharmacy, Volume 47, Issue 6, 1 June 1990, Pages 1382–1398, https://doi.org/10.1093/ajhp/47.6.1382 Published: 01 June 1990
High peak power green light generation using ytterbium fiber amplifier and lithium triborate crystal (2011)

We reported on a master-oscillator fiber power amplifier (MOPA) system and a second-harmonic generation (SHG) system. Actively Q-switched Nd:YVO4 laser was used as a seed source of light pluses. The MOPA system produced as much as 76 kW of peak power at wavelength of 1064 nm, with 35 kHz repetition rate and 20 ns pulse duration. With a lithium triborate (LBO) crystal at type II phase matching, a maximum 16 kW of peak power had been demonstrated. The fiber nonlinear effects such as stimulate Brillouin scattering (SBS) and stimulate Raman scattering (SRS) and the SHG conversion efficiency were investigated.
Estudo de avaliação das habilidades de vida independente entre usuários da rede de atenção psicossocial do Rio Grande do Sul (2016)

Resumo Introdução Este estudo objetivou descrever as habilidades de vida independente de uma amostra de usuários da rede de atenção psicossocial do Rio Grande do Sul, identificando as áreas com menores e maiores desempenhos. Métodos A amostra foi de 390 usuários. A fim de determinar o grau de habilidades da vida cotidiana de pacientes psiquiátricos, foi utilizada a escala Independent Living Skills Survey (ILSS), que avalia o desempenho dos sujeitos em nove áreas de atividade da vida cotidiana. As análises foram conduzidas no pacote estatístico Stata 11, e utilizou-se a estatística descritiva para apresentação dos resultados. Resultados O escore global encontrado para habilidades de vida independente foi de 2,44, com desvio-padrão (DP) de 0,90. Os itens de maior escore foram aqueles relacionados à alimentação, cuidados pessoais e transporte. Os itens com menores escores foram emprego, lazer e administração de dinheiro. Conclusão As habilidades de vida independente entre os indivíduos incluídos neste estudo foram maiores do que em outras pesquisas realizadas anteriormente acerca do tema.
HAUSP-nucleolin interaction is regulated by p53-Mdm2 complex in response to DNA damage response (2015)

HAUSP (herpes virus-associated ubiquitin specific protease, known as ubiquitin specific protease 7), one of DUBs, regulates the dynamics of the p53 and Mdm2 network in response to DNA damage by deubiquitinating both p53 and its E3 ubiquitin ligase, Mdm2. Its concerted action increases the level of functional p53 by preventing proteasome-dependent degradation of p53. However, the protein substrates that are targeted by HAUSP to mediate DNA damage responses in the context of the HAUSP-p53-Mdm2 complex are not fully identified. Here, we identified nucleolin as a new substrate for HAUSP by proteomic analysis. Nucleolin has two HAUSP binding sites in its N- and C-terminal regions, and the mutation of HAUSP interacting peptides on nucleolin disrupts their interaction and it leads to the increased level of nucleolin ubiquitination. In addition, HAUSP regulates the stability of nucleolin by removing ubiquitin from nucleolin. Nucleolin exists as a component of the HAUSP-p53-Mdm2 complex, and both Mdm2 and p53 are required for the interaction between HAUSP and nucleolin. Importantly, the irradiation increases the HAUSP-nucleolin interaction, leading to nucleolin stabilization significantly. Taken together, this study reveals a new component of the HAUSP-p53-Mdm2 complex that governs dynamic cellular responses to DNA damage.
Routinizing Frame Analysis through the Use of CAQDAS (2004)

Even though frame analysis has become a popular analytical framework in media studies and social movement research, the methodological underpinnings of the empirical identification of frames lack systematization and have consequently remained underdeveloped. This paper consolidates recent advances in the empirical measurement of frames and explores, in how far computer-assisted qualitative data analysis software (CAQDAS) can extend on these methodologies. Because framing has become a fairly widely used but ill-defined concept, the paper will start with a brief delineation of framing theory as it is understood for present purposes. Next, current attempts to measure frames empirically in a systematic fashion will be discussed and a methodology, which synthesizes some of these approaches will be proposed. This methodology attempts in a first step to draw on existing knowledge on metanarratives to avoid a purely inductive identification of frames. Additionally, automatic word mapping tools such as Leximancer, Sphinx Survey Lexica are suggested as interpretative aids. In a second step, the analyst identifies a set of keywords, key phrases, and possibly audial or visual symbols that indicate frames in his data. These indicators are then used in a third step to semiautomatically identify frames in the remainder of the data. Keywords that might acquire different meanings in different contexts are inspected in their contexts by the analyst, who decide on their coding. This method avoids both the rigidities that come with fully automatic keyword clustering, which may lead to the inclusion non-interpretable keywords as well as the exclusion of so-called stop words such as prepositions and articles, which under certain circumstances might indeed be the strongest indicators for certain frames. At the same time it allows for a degree of routinization and systematization in frame analysis, whose quality has notoriously depended on the creativity of the framing researchers. Five CAQDAS – ATLAS.ti, Kwalitan, MAXqda, NVivo, and Qualrus – are examined with respect to their usability in this type of framing research.
Silver and nanoparticles of silver in wound dressings: a review of efficacy and safety (2011)

Wound infections present a significant clinical challenge, impacting on patient morbidity and mortality, with significant economic implications. Silver-impregnated wound dressings have the potential to reduce both wound bioburden and healing time. The silver ion Ag+ is the active antimicrobial entity; it can interfere with thiol (-SH) groups and provoke the generation of reactive oxygen species (ROS), a major contributor to its antibacterial efficacy. Recently, silver nanoparticles have gained considerable interest in wound bioburden reduction and in anti-inflammation, as they can release Ag+ ions at a greater rate than bulk silver, by virtue of their large surface area. If released from dressings, they also have the potential to cross biological compartments. This review aims to consolidate recent findings as to the efficacy and safety of different formulations of silver used as an antiseptic agent in dressings, summarising the features of silver nanomaterials, with particular attention to the dose-dependencies for biological effects, highlighting the need for information on their uptake and potential biological effects.
Lignin Structure and Reactions (1966)

A schematic formula for lignin has evolved from analytical observations and from studies of biochemically duplicated lignin and its intermediates. By dehydrogenation, coniferyl alcohol alone forms more than 30 intermediates (oligolignols) which give detailed information about the constitution of lignin. Most of the intermediates have been isolated and elucidated. They are listed here. Recently, it became evident that rather labile benzyl aryl ether links occur in lignin. This led to experiments on the mild hydrolysis of lignin. Several oligolignols have been isolated from wood, some of which are identical with the intermediates; others supplement them. Work done in Heidelberg during the last 12 years is reported. Results from other laboratories are mentioned only when they apply directly to these topics.
Human tissue-engineered skeletal muscle: a novel 3D in vitro model for drug disposition and toxicity after intramuscular injection (2018)

The development of laboratory-grown tissues, referred to as organoids, bio-artificial tissue or tissue-engineered constructs, is clearly expanding. We describe for the first time how engineered human muscles can be applied as a pre- or non-clinical model for intramuscular drug injection to further decrease and complement the use of in vivo animal studies. The human bio-artificial muscle (BAM) is formed in a seven day tissue engineering procedure during which human myoblasts fuse and differentiate to aligned myofibers in an extracellular matrix. The dimensions of the BAM constructs allow for injection and follow-up during several days after injection. A stereotactic setup allows controllable injection at multiple sites in the BAM. We injected several compounds; a dye, a hydrolysable compound, a reducible substrate and a wasp venom toxin. Afterwards, direct reflux, release and metabolism were assessed in the BAM constructs in comparison to 2D cell culture and isolated human muscle strips. Spectrophotometry and luminescence allowed to measure the release of the injected compounds and their metabolites over time. A release profile over 40 hours was observed in the BAM model in contrast to 2D cell culture, showing the capacity of the BAM model to function as a drug depot. We also determined compound toxicity on the BAMs by measuring creatine kinase release in the medium, which increased with increasing toxic insult. Taken together, we show that the BAM is an injectable human 3D cell culture model that can be used to measure release and metabolism of injected compounds in vitro.