gebhart/patrepeval huggingface dataset:
Estimation and tracking of fundamental, 2nd and 3d harmonic frequencies for spectrogram normalization in speech recognition (2012)

Estimation and tracking of fundamental, 2nd and 3d harmonic frequencies for spectrogram normalization in speech recognition A stable and accurate estimation of the fundamental frequency (pitch, F 0) is an important requirement in speech and music signal analysis, in tasks like automatic speech recognition and extraction of target signal in noisy environment. In this paper, we propose a pitch-related spectrogram normalization scheme to improve the speaker - independency of standard speech features. A very accurate estimation of the fundamental frequency is a must. Hence, we develop a non-parametric recursive estimation method of F 0 and its 2nd and 3d harmonic frequencies in noisy circumstances. The proposed method is different from typical Kalman and particle filter methods in the way that no particular sum of sinusoidal model is used. Also we tend to estimate F0 and its lower harmonics by using novel likelihood function. Through experiments under various noise levels, the proposed method is proved to be more accurate than other conventional methods. The spectrogram normalization scheme makes a mapping of real harmonic structure to a normalized structure. Results obtained for voiced phonemes show an increase in stability of the standard speech features - the average within-phoneme distance of the MFCC features for voiced phonemes can be decreased by several percent.
Global range alignment for ISAR (2003)

A new technique is developed for range alignment in inverse synthetic aperture radar (ISAR) imaging. The shifts made to the echoes are modeled as a polynomial, and the coefficients of this polynomial are chosen to optimize a global quality measure of range alignment. This technique is robust against noise and target scintillation, and avoids error accumulation. In addition, the shift in the time domain is implemented by introducing a phase ramp in the frequency domain, which removes the limitation of integer steps.
Factor XII-dependent Contact Activation on Endothelial Cells and Binding Proteins gC1qR and Cytokeratin 1 (2001)

Although proteins of the kinin-forming pathway are bound along the surface of endothelial cells, the mechanism of activation of this proteolytic cascade is unclear. Endothelial cell surface proteins, gC1qR and cytokeratin 1, are capable of binding Factor XII and high molecular weight kininogen (HK) in a zinc-dependent reaction thus we considered the possibility that these proteins might catalyze initiation of the cascade. Incubation of Factor XII, prekallikrein, and HK with gC1qR or cytokeratin 1 leads to a zinc-dependent and Factor XII-dependent conversion of prekallikrein to kallikrein. We also demonstrate that normal plasma is capable of activating upon interaction with the cells whereas plasma deficient in Factor XII, prekallikrein and HK do not activate. Normal plasma activation was inhibitable by antibody to gC1qR and cytokeratin 1. Thus, gC1qR and cytokeratin 1, represent potential initiating surfaces for activation of the plasma kinin-forming cascade and may do so as a result of their expression along cell surfaces.
MLL Gene Alterations in Acute Myeloid Leukaemia (11q23/MLL+ AML) (2013)

The percentage of translocation in AML subtypes. Values in brackets indicate the frequency within the morphological or immunological disease subtype. ** Percentage refers to the frequency of reciprocal translocation chromosome products resulting. AML -acute myelogenous leukemia, t-AML (therapy-related AML) -AML associated with therapy, MDS -myelodysplastic syndrome; CMMoL -chronic myelomonocytic leukemia
Quantitative bioimage analytics enables measurement of targeted cellular stress response induced by celastrol-loaded nanoparticles (2019)

The cellular stress response, which provides protection against proteotoxic stresses, is characterized by the activation of heat shock factor 1 and the formation of nuclear stress bodies (nSBs). In this study, we developed a computerized method to quantify the formation and size distribution of nSBs, as stress response induction is of interest in cancer research, neurodegenerative diseases, and in other pathophysiological processes. We employed an advanced bioimaging and analytics workflow to enable quantitative detailed subcellular analysis of cell populations even down to single-cell level. This type of detailed analysis requires automated single cell analysis to allow for detection of both size and distribution of nSBs. For specific induction of nSB we used mesoporous silica nanoparticles (MSNs) loaded with celastrol, a plant-derived triterpene with the ability to activate the stress response. To enable specific targeting, we employed folic acid functionalized nanoparticles, which yields targeting to folate receptor expressing cancer cells. In this way, we could assess the ability to quantitatively detect directed and spatio-temporal nSB induction using 2D and 3D confocal imaging. Our results demonstrate successful implementation of an imaging and analytics workflow based on a freely available, general-purpose software platform, BioImageXD, also compatible with other imaging modalities due to full 3D/4D and high-throughput batch processing support. The developed quantitative imaging analytics workflow opens possibilities for detailed stress response examination in cell populations, with significant potential in the analysis of targeted drug delivery systems related to cell stress and other cytoprotective cellular processes.
Improvement of the gain of MOS amplifiers (1979)

Techniques for improving the gain of MOS amplifiers are discussed. These techniques depend on technology used. An experimental single stage amplifier was realized using CMOS transistor arrays which achieved gain of 3200.
A polymorphism in the endothelin-A receptor gene predicts survival in patients with idiopathic dilated cardiomyopathy (2001)

Our results suggest that genetic variation in the ETA receptor predicts survival in dilated cardiomyopathy patients, which might have important consequences for the identification of high-risk individuals.
Influence of Biofuel Combustion Fly Ash on the Properties of Concrete (2015)

Sciendo provides publishing services and solutions to academic and professional organizations and individual authors. We publish journals, books, conference proceedings and a variety of other publications.
Exxon catalytic coal gasification process predevelopment program. Monthly report, December, 1977 (1978)

Solids analyses for all FBG yield periods have been completed. The off-line material balance computer program has been used to reconcile the data from 11 of the 18 FBG yield periods. Bench scale fixed bed gasification studies indicated that KOH is equivalent to K/sub 2/CO/sub 3/ as a gasification catalyst. However, K/sub 2/S shows lower catalytic activity than K/sub 2/CO/sub 3/. Also, Wyodak coal catalyzed with either KOH or K/sub 2/CO/sub 3/ shows the same reactivity as Illinois coal impregnated with these catalysts. Work continued on the Catalytic Coal Gasification Commercial Plant Study Design. Process bases and heat and material balances have been completed. Current efforts are directed toward calculation of investment, operating and product gas costs. A screening study was made of alternative methods of producing makeup KOH by electrolysis of KCl. This study showed that KOH could be produced in diaphragm cells or membrane cells at significantly lower cost than the current price of purchased KOH. Another screening study showed that the cost of potassium recovered by calcium hydroxide digestion is about 68% of the cost of purchased KOH. Further work will be required to better define the relative costs of recovered vs. manufactured KOH. Studies to evaluate alternative acid gas removal processes were completed. An evaluation of conventional acid gas scrubbing techniques indicated that selective heavy glycol scrubbing is the preferred process for a catalytic gasification plant, closely followed by non-selective hot potassium carbonate scrubbing. An evaluation of cryogenic fractionation for acid gas removal showed that this technique cannot be carried out without CO/sub 2/ freezing out in the fractionator.
Intramural Plasminogen Activator Inhibitor Type-1 and Coronary Atherosclerosis (2003)

Altered expression of plasminogen activator inhibitor type-1 in vessel walls, reviewed here, might affect coronary atherogenesis. Upregulation might exacerbate vasculopathy by potentiating thrombosis and by inhibiting vascular smooth muscle cell migration, resulting in attenuation of thickness of elaborated fibrous caps implicated in the vulnerability of atheroma to rupture.
[The basic and applied study on the epidermal growth factor]. (2001)

This article reviews the results of the basic research about epidermal growth factor and its receptor, and the development of the novel drug, EGF eyedrop, that containing chemically synthesized EGF gene, the construction of EGF expression vector, the transformation of the host cells, the purification of the recombinant protein EGF, the preparation of three batches of the EGF product and identification, the preclinical and clinical trials. Relevant studies show that recombinant EGF consisting of 51 amino acids can be secreted into the medium under the control of the alpha factor leading sequence in the yeast cells. The EGF can accelerate the growth of corneal-limbal epithelial cells and the healing of an alkali burned corneal. The EGF can be used in curing oral cavity ulcer and skin burned wound. And it has the preventive effects on experimental duodenal ulcer of rat. The antiserum was made for test of the concentration of blood EGF and urine EGF by RIA. Data from studies demonstrate the inhibition effect of EGF on the growth of tumor cells, such as A431 and BT325 cells in the presence of high EGF concentration (> 10 ng/ml). The expression of EGFR and DNA ploidy in renal carcinoma has clinical significance. Crystallization and preliminary x-ray diffraction studies of the EGF has been made. The MW of the EGF product is 6000, and the pI is about 4.6 and it has correct N-terminal amino acids sequences, immunogenicity and biological activity. There is no vestige of the DNA of the yeast cells. Animal experiments reveal that there is no cumulation of the EGF in the body, and EGF can promote corneal epithelial healing. There is no toxicological effect during cornea wound healing of rabbit. A randomized, double-blind, placebo-controlled, multi-center clinical trial was conducted in four hospitals to assess safety, ocular tolerance and efficacy of an ophthalmic solution of EGF for 200 cases of cornea transplantation and 247 cases of nebulae. Unequivocal results were obtained as the eyedrop really accelerate the wounded cornea healing. So, the EGF eyedrop as a novel drug of class I is approved by the National Drug Administration, and this is the first gene engineering drug that come from yeast expression system in China.
Functions of Individual .gamma.-Carboxyglutamic Acid (Gla) Residues of Human Protein C. Determination of Functionally Nonessential Gla Residues and Correlations with Their Mode of Binding to Calcium (1994)

Previous studies from this laboratory have been directed toward elucidation of the roles of individual gamma-carboxyglutamic acid (Gla) residues in Gla domain-related Ca(2+)-directed properties of human protein C (PC) and activated protein C (APC). On the basis of results using recombinant variants of PC containing highly conservative (Asp) mutations of individual Gla residues, it was previously proposed that Gla6, Gla14, and Gla19 may not be essential for properties associated with the Ca(2+)-dependent conformation of the Gla domain of these proteins. In this study, we have demonstrated that radical mutations to Val of Gla residues 14 and 19 resulted in 94% and 82%, respectively, of the Gla domain-related, Ca(2+)- and phospholipid- (PL-) dependent anticoagulant (APTT) activity of wild-type recombinant (wtr) APC, while [Gla6-->Val]r-APC showed a complete loss of this same activity. The more conservative mutant [Gla6-->Gln]r-APC possessed 4% of the APTT activity of wtr-APC, whereas [Gla6-->Asp]r-APC was nearly fully active. As with wtr-PC, both [Gla6-->Val]r-PC and [Gla6-->Gln]r-PC displayed Ca(2+)-dependent intrinsic fluorescence quenching, suggesting that they adopted a Ca(2+)-induced conformation. However, Ca2+ titration data suggested that these conformations were not identical to that undergone by wtr-PC. In addition, the Ca(2+)-mediated binding parameters of [Gla6-->Val]r-PC and [Gla6-->Gln]r-PC to acidic PL vesicles were found to be defective. These data were interpreted at the molecular level using a model for the Gla domain of PC based on the X-ray crystal structure of the Ca2+/bovine prothrombin fragment 1 complex.(ABSTRACT TRUNCATED AT 250 WORDS)
Genetic analysis of the most acidic 11S globulin subunit and related characters in soybean seeds. (1983)

The A5 subunit is the most acidic subunit of soybean 11S globulin. The inheritance of A5 Subunit was studied by analyzing electrophoretically the crude 11S globullns in single seeds of F1 and F2 generations of four crosses. A part of these seeds were used to investigate the genetic relationships between A5 subunit and other characters, i, e., basic subunits, 11S globulin composition, 11S/7S ratio and seed protein content. The content of A5 Subunit was under the control of the alleles (Gl1, gl1) at one locus and highest in Gl1Gl1, about half of Gl1Gl1 in Gl1gl1 and zero in gl1gl1. The presence or absence of A5 Subunit and two specific basic subunits was entirely coordinated with each other, therefore, it was suggested that the structural genes coding for A5 subunit and two specific basic subunits were closely linked with each other to constitute Gl1 or the synthesis of these subunits was synchronously controlled by Gl1 and gl1. The presence of these subunits was accompanied by the most acidic 11S globulin molecule which is missing in gl1gl1. 11S/7S ratio was closely related to Gl1 gene dosage and highest in Gl1Gl1, intermediate in Gl1gl1 and lowest in gl1gl1. These results show that Gl1 is responsible for increasing heterogeniety of 11S globulin molecular species and raising 11S/7S ratio. There were no significant differences in protein content among three genotypes and 11S/7S ratio was not correlated with seed protein content so that these genes will have no effect on protein content and increasing 11S globulin may not decrease protein content.
Color Signal Distortions in Envelope Type of Second Detectors (1957)

When an NTSC color signal transmission is detected by an envelope type of second detector, several distortion components can be produced. In saturated color areas the detected luminance signal may be lower than the intended level, and additionally a 920-kc color-sound beatnote may result.
Flow Separation Control Over a Ramp Using Sweeping Jet Actuators (2014)

Flow separation control on an adverse-pressure-gradient ramp model was investigated using various flow-control methods in the NASA Langley 15-Inch Wind Tunnel. The primary flow-control method studied used a sweeping jet actuator system to compare with more classic flow-control techniques such as micro-vortex generators, steady blowing, and steady- and unsteady-vortex generating jets. Surface pressure measurements and a new oilflow visualization technique were used to characterize the effects of these flow-control actuators. The sweeping jet actuators were run in three different modes to produce steady-straight, steady-angled, and unsteady-oscillating jets. It was observed that all of these flow-control methods are effective in controlling the separated flows on the ramp model. The steady-straight jet energizes the boundary layer by momentum addition and was found to be the least effective method for a fixed momentum coefficient. The steady-angled jets achieved better performance than the steady-straight jets because they generate streamwise vortices that energize the boundary layer by mixing high-momentum fluid with near wall low-momentum fluid. The unsteady-oscillating jets achieved the best performance by increasing the pressure recovery and reducing the downstream flow separation. Surface flow visualizations indicated that two out-of-phase counter-rotating vortices are generated per sweeping jet actuator, while one vortex is generated per vortex-generating jets. The extra vortex resulted in increased coverage, more pressure recovery, and reduced flow separation.
Introduction: the West (1988)

The world of the Christian fathers from Ambrose to Isidore of Seville had come to differ profoundly from the world of the early Apologists, of Origen and Tertullian. The transformations which produced these differences came in two great waves: the first, sweeping across the whole of the Roman world, is the crisis of the third century from which Roman society was to emerge into a new stability in the fourth, a stability won through extensive re-organisation and accompanied by far-reaching changes not only in the administrative and social structure of the Empire, but also in its traditional culture and religion. These changes, though their incidence differed from region to region, affected the Empire everywhere. The second wave rolled mainly over the Western provinces: the Germanic invaders who settled within imperial frontiers came to create their own, eventually independent, kingdoms on what had been Roman soil. Both waves radically altered the social and political structures of Western Europe, and also the cultural conditions in which reflection on those structures could take place.
Designing CXCL8-based decoy proteins with strong anti-inflammatory activity <i>in vivo</i> (2013)

IL (interleukin)-8 [CXCL8 (CXC chemokine ligand 8)] exerts its role in inflammation by triggering neutrophils via its specific GPCRs (G-protein-coupled receptors), CXCR1 (CXC chemokine receptor 1) and CXCR2, for which additional binding to endothelial HS-GAGs (heparan sulphate-glycosaminoglycans) is required. We present here a novel approach for blocking the CXCL8-related inflammatory cascade by generating dominant-negative CXCL8 mutants with improved GAG-binding affinity and knocked-out CXCR1/CXCR2 activity. These non-signalling CXCL8 decoy proteins are able to displace WT (wild-type) CXCL8 and to prevent CXCR1/CXCR2 signalling thereby interfering with the inflammatory response. We have designed 14 CXCL8 mutants that we subdivided into three classes according to number and site of mutations. The decoys were characterized by IFTs (isothermal fluorescence titrations) and SPR (surface plasmon resonance) to determine GAG affinity. Protein stability and structural changes were evaluated by far-UV CD spectroscopy and knocked-out GPCR response was shown by Boyden chamber and Ca2+ release assays. From these experiments, CXCL8(Δ6F17KF21KE70KN71K) emerged with the most promising in vitro characteristics. This mutant was therefore further investigated in a murine model of mBSA (methylated BSA)-induced arthritis in mice where it showed strong anti-inflammatory activity. Based on these results, we propose that dominant-negative CXCL8 decoy proteins are a promising class of novel biopharmaceuticals with high therapeutic potential in inflammatory diseases.
Modeling the dynamics of nonlinear inductor circuits (1994)

The Jiles-Atherton (J-A) model is applied to the problem of describing the dynamics of a nonlinear circuit driven by a square wave voltage source and comprising a linear resistor and capacitor in series with a nonlinear inductor, whose core displays saturation and hysteresis. The presence of hysteresis is shown to increase the order of the circuit by one. Period-multiplication and chaos are observed and excellent agreement is obtained between experiment and simulation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Liquid Crystal‐Induced Myoblast Alignment (2019)

The ability to control cell alignment represents a fundamental requirement toward the production of tissue in vitro but also to create biohybrid materials presenting the functional properties of human organs. However, cell cultures on standard commercial supports do not provide a selective control on the cell organization morphology, and different techniques, such as the use of patterned or stimulated substrates, are developed to induce cellular alignment. In this work, a new approach toward in vitro muscular tissue morphogenesis is presented exploiting liquid crystalline networks. By using smooth polymeric films with planar homogeneous alignment, a certain degree of cellular order is observed in myoblast cultures with direction of higher cell alignment corresponding to the nematic director. The molecular organization inside the polymer determines such effects since no cell organization is observed using homeotropic or isotropic samples. These findings represent the first example of cellular alignment induced by the interaction with a nematic polymeric scaffold, setting the stage for new applications of liquid crystal polymers as active matter to control tissue growth.
In search of the best clock (1989)

The authors show that significant gains in clock performance have been and can be obtained through the algorithms which optimize the clock readings and through international comparisons now available via satellite. Algorithms for processing are more important than the proportionate attention generally given them. In fact, to date, one of the main ways the authors have been able to investigate some of the long-term performance aspects of the millisecond pulsar, PSR 1937+21, is by using such optimization algorithms. The authors assert that since there are indications in the pulsar data of variations which could be explained as arising from the influence of gravitational waves, these long-term stability studies take on a new importance. Improved long-term stability of earth-bound clock systems will significantly assist the study of the incredibly stable spin rates of these neutron stars.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>