gebhart/patrepeval huggingface dataset:
Weaponizing human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) for 21st century cancer therapeutics (2016)

De-regulated <i>EFEMP1</i> gene expression in solid tumors has been widely reported with conflicting roles. We dissected EFEMP1 to identify domains responsible for its cell context-dependent dual functions, with the goal being to construct an EFEMP1-derived tumor-suppressor protein (ETSP) that lacked tumor-promoting function. Exon/intron boundaries of <i>EFEMP1</i> were used as boundaries of functional modules in constructing EFEMP1 variants, with removal of various module(s), and/or mutating an amino acid residue to convert a weak integrin binding-site into a strong one. A series of <i>in vitro</i> assays on cancerous features, and subcutaneous and intracranial xenograft-formation assays, were carried out for effects from overexpression of wild-type and variant forms of EFEMP1 in two glioma subpopulations characterized as tumor mass-forming cells (TMCs) or stem-like tumor initiating cells (STICs), where EFEMP1 showed cellcontext- dependent dual functions. One of the EFEMP1 variants was identified as the sought-after ETSP, which had a stronger tumor-suppression function in TMCs by targeting EGFR and angiogenesis, and a new tumor-suppression function in STICs by targeting NOTCH signaling and MMP2-mediated cell invasion. Therefore, ETSP may form the basis for further important research to develop a novel cancer therapy to treat many types of cancer by its tumor suppressor effect in the extracellular matrix compartment.
Visible light induced water cleavage in colloidal solutions of chromium-doped titanium dioxide particles (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVisible light induced water cleavage in colloidal solutions of chromium-doped titanium dioxide particlesEnrico Borgarello, John Kiwi, Michael Graetzel, Ezio Pelizzetti, and Mario ViscaCite this: J. Am. Chem. Soc. 1982, 104, 11, 2996–3002Publication Date (Print):June 1, 1982Publication History Published online1 May 2002Published inissue 1 June 1982https://pubs.acs.org/doi/10.1021/ja00375a010https://doi.org/10.1021/ja00375a010research-articleACS PublicationsRequest reuse permissionsArticle Views2234Altmetric-Citations528LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Formation, Chemical Composition, and Structure of Polyelectrolyte−Nanoparticle Multilayer Films (2002)

The buildup and structure of multilayer films containing cationic polyelectrolyte and silica nanoparticles have been studied by means of ellipsometry, atomic force microscopy, and X-ray photoelectron spectroscopy. Emphasis was placed on the effect of ionic strength on the adsorption behavior and structure formation. Consecutive exposure of a silica substrate to low ionic strength solutions containing polyelectrolyte and nanoparticles resulted in the formation of stable adsorbed films with a reproducible stratified multilayer structure. The films formed in high ionic strength solutions were initially much thicker but also clearly less stable. A significant desorption was observed to take place in conjunction with the second exposure to silica nanoparticles. The effect of electrolyte concentration is discussed in terms of a salt-induced glass−liquid transition, above which the relaxation rate in the adsorbed composite film increases and thereby hinders the formation of multilayer structures.
The Challenge of <i>Pneumocystis carinii</i> Culture1 (1993)

Published and unpublished data on the cultivation of P. carinii were reviewed by a panel of investigators convened by the National Institutes of Health. Although several cell culture systems allow propagation of P. carinii for a limited time with modest rates of replication, these have not proved adequate for isolation of P. carinii in sufficient quantity to explore important basic biological investigation. Attempts at cell-free culture have yielded only transient proliferation. Because much of the unsuccessful work on cultivation of the organism has been unpublished, the panel agreed that these data may be useful to other investigators in designing experimental strategies for cultivation. Therefore, the purpose of this report is to make available this information to researchers, lest others unknowingly repeat unsuccessful methods. It is hoped that by documenting the history and the complexities of Pneumocystis culture, renewed interest and efforts will be directed toward this fundamental scientific challenge.
Novel metal ion surface modification technique (1991)

We describe a method for applying metal ions to the near-surface region of solid materials. The added species can be energetically implanted below the surface or built up as a surface film with an atomically mixed interface with the substrate; the metal ion species can be the same as the substrate species or different from it, and more than one kind of metal species can be applied, either simultaneously or sequentially. Surface structures can be fabricated, including coatings and thin films of single metals, tailored alloys, or metallic multilayers, and they can be implanted or added onto the surface and ion beam mixed. We report two simple demonstrations of the method: implantation of yttrium into a silicon substrate at a mean energy of 70 keV and a dose of 1×1016 atoms/cm2, and the formation of a titanium-yttrium multilayer structure with ion beam mixing to the substrate.
Coherent beam-beam effects (2002)

We have performed a strong-strong simulation to investigate the effect of a beam-beam interaction on the motion of circulating beams in a 50 GeV /spl times/ 50 GeV muon-collider ring. The phase-slip factor /spl eta//sub 1/ in the muon-collider ring is approximately -1 /spl times/ 10/sup -6/ so that the synchrotron motion is nearly frozen during the muon-storage time. Thus, the energy variation of a particle in the beam due to the beam-beam interaction accumulates from turn to turn, resulting in increase in the energy spread of the beam. Through the strong-strong simulation, we have investigated the parametric dependencies of the energy spread and the beam size induced by the beam-beam interaction on various parameters. It is shown that the induced energy spread due to the beam-beam interaction affects the growth of the beam size. It is also shown that compensation of the energy spread by introducing one additional rf cavity in the ring can reduce the growth of the beam size.
An overview about erythrocyte membrane (2010)

In the sixties and seventies, erythrocytes or red blood cells (RBCs) were extensively studied. Much has been learnt particularly concerning their metabolism and gas transporter function.In the past decade, the use of new approaches and methodologies, such as proteomic analysis, has contributed for a renewed interest on the erythrocyte. Recent studies have provided us with a more detailed and comprehensive picture on the composition and organization of its cellular membrane that will be the main subject of this minireview. Unexpectedly, it has been recognized that this cell expresses several adhesion molecules on its surface, like other cellular types such blood circulating cells or endothelial cells. Taking into consideration the cellular functions of the erythrocyte, the clarification of the role of those adhesion molecules may in the future open new horizons for the biological significance of this cellular player.
IL-10 induces aberrant deletion of dendritic cells by natural killer cells in the context of HIV infection (2010)

Persistent levels of IL-10 play a central role in progressive immune dysfunction associated with chronic viral infections such as HIV, but the underlying mechanisms are poorly understood. Because IL-10 affects the phenotypic and functional properties of DCs, which are responsible for initiating adaptive immune responses, we investigated whether IL-10 induces changes in DC phenotype and function in the context of HIV infection. Here, we show that IL-10 treatment of immature and mature human DCs in culture induced contrasting phenotypic changes in these populations: immature DCs exhibited aberrant resistance to NK cell-mediated elimination, whereas mature DCs exhibited increased susceptibility to NKG2D-dependent NK elimination. Treatment of immature and mature DCs with HIV resulted in potent IL-10 secretion and the same phenotypic and functional changes observed in the IL-10-treated cells. Consistent with these in vitro data, LNs isolated from individuals infected with HIV exhibited aberrant accumulation of a partially "immature" DC population. Together, these data suggest that the progressive immune dysfunction observed in chronic viral infections might be caused in part by IL-10-induced reversal of DC susceptibility to NK cell-mediated elimination, resulting in the accumulation of poorly immunogenic DCs in LNs, the sites of adaptive immune response induction.
Capacitive fiber-meshed transducers for touch and proximity-sensing applications (2005)

Capacitive sensing is used in manufacturing E-textiles for touch and proximity-sensing applications. The common approach is to construct electrodes on top of a nonconductive fabric structures. Woven and knitted fabric structures are used for the construction; metallic wire and conductive coated fibers are primarily used. Due to the performance degradation and poor comfort of these constructions, we have constructed electrodes with inherently conductive polymers and multifilament metallic fibers by integrating them into fiber-meshed structures such that the electrodes are a part of the nonconductive base structure. We have used capacitive and resistive measurement techniques for the detection. Out of many mechanical methods of fiber-integrating processors, we have used flat bed-knitting technology and Jacquard weaving technology. In this paper, we have discussed the construction, sensing, and applications of capacitive fiber-meshed transducers and their applications.
Expression of 4-Integrin Defines the Earliest Precursor of Hematopoietic Cell Lineage Diverged From Endothelial Cells (1999)

Embryonic stem cells can differentiate in vitro into hematopoietic cells through two intermediate stages; the first being FLK1(+) E-cadherin- proximal lateral mesoderm and the second being CD45(-) VE-cadherin+ endothelial cells. To further dissect the CD45(-) VE-cadherin+ cells, we have examined distribution of alpha4-integrin on this cell population, because alpha4-integrin is the molecule expressed on hematopoietic stem cells. During culture of FLK1(+) E-cadherin- cells, CD45(-) VE-cadherin+ alpha4-integrin- cells differentiate first, followed by alpha4-integrin+ cells appearing in both CD45(-) VE-cadherin+ and CD45(-) VE-cadherin- cell populations. In the CD45(-) VE-cadherin+ cell population, alpha4-integrin+ subset but not alpha4-integrin- subset had the potential to differentiate to hematopoietic lineage cells, whereas endothelial cell progenitors were present in both subsets. The CD45(-) VE-cadherin- alpha4-integrin+ cells also showed hematopoietic potential. Reverse transcription-polymerase chain reaction analyses showed that differential expression of the Gata2 and Myb genes correlated with the potential of the alpha4-integrin+ cells to give rise to hematopoietic cell differentiation. Hematopoietic CD45(-) VE-cadherin+ alpha4-integrin+ cells were also present in the yolk sac and embryonic body proper of 9.5 day postcoitum mouse embryos. Our results suggest that the expression of alpha4-integrin is a marker of the earliest precursor of hematopoietic cell lineage that was diverged from endothelial progenitors.
A Hybrid Energy Storage System Using Series-Parallel Reconfiguration Technique (2016)

Technology advancements enable and encourage higher system electrifications in various applications. More electrified applications need more capable and higher performing sources of energy in terms of power delivery, power regeneration, and energy capacity. For example, in electric, hybrid electric, and plug-in hybrid electric vehicle applications (EVs, HEVs, and PHEVs), the power and energy ratings of the vehicle energy storage system (ESS) have a direct impact on the vehicle performance. Many researchers investigated and studied various aspects of hybrid energy storage systems (HESS) wherein multiple ESSs are combined together to share system loads, increase ESS capabilities, and cycle life. Various configurations and their application specific topologies were also proposed by other researchers; the potential of HESS has been proven to be very promising. In this research, the goal is to present the theory of a HESS configuration that has not been discovered thus far. This HESS configuration is called a series-parallel reconfigurable HESS (SPR-HESS) since it is capable of recombining multiple storage systems into different series, parallel, or series-parallel configurations, via power electronic converters, to accommodate different operation modes and load requirements. Simulations, as well as experimental verifications, are presented in this thesis.
An air bearing minimizing the effects of slider skew angle (1989)

A minislider incorporating a single transverse slot across both rails is presented which greatly reduces the effect of slider skew. The slots, in conjunction with wide rails, allow a slider mounted on a rotary actuator to access the entire disk at a spacing of 100 to 120 nm. Skew decreases the head-to-disk spacing of conventional minisliders and is a particular problem in disk drives equipped with rotary actuators as slider rail width is decreased. The flying characteristics of this slider are investigated by a computer simulation program and by measurements on actual sliders with a multichannel laser interferometer. >
The hydroboration of silylacetylenes. The "silyl-Markovnikov" hydroboration route to pure (Z)-1-(2-borylvinyl)silanes and .beta.-keto silanes (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe hydroboration of silylacetylenes. The "silyl-Markovnikov" hydroboration route to pure (Z)-1-(2-borylvinyl)silanes and .beta.-keto silanesJohn A. Soderquist, Juan C. Colberg, and Luis Del ValleCite this: J. Am. Chem. Soc. 1989, 111, 13, 4873–4878Publication Date (Print):June 1, 1989Publication History Published online1 May 2002Published inissue 1 June 1989https://pubs.acs.org/doi/10.1021/ja00195a046https://doi.org/10.1021/ja00195a046research-articleACS PublicationsRequest reuse permissionsArticle Views1303Altmetric-Citations81LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A 10.3125-Gbit/s SiGe BiCMOS Burst-Mode 3R Receiver for 10G-EPON Systems (2007)

The first burst-mode 3R receiver operating at 10 Gbit/s using monolithic ICs is reported. We achieved -18.0-dBm sensitivity and 75-ns responsivity. This is the highest sensitivity among all reported 10-Gbit/s-class burst-mode receivers.
Speaker adaptation for large vocabulary speech recognition systems using speaker Markov models (1989)

An alternative approach to speaker adaptation for a large-vocabulary hidden-Markov-model-based speech recognition system is described. The goal of this investigation was to train the IBM speech recognition system with only five minutes of speech data from a new speaker instead of the usual 20 minutes without the recognition rate dropping by more than 1-2%. The approach is based on the use of a stochastic model representing the different properties of the new speaker and an old speaker for which the full training set of 20 minutes is available. It is called a speaker Markov model. It is shown how the parameters of such a model can be derived and how it can be used for transforming the training set of the old speaker in order to use it in addition to the short training set of the new speaker. The adaptation algorithm was tested with 12 speakers. The average recognition rate dropped from 96.4% to 95.2% for a 5000-word vocabulary task. The decoding time increased by a factor of 1.35; this factor is often 3-5 if other adaptation algorithms are used.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Life without DNA repair (1997)

The advent of gene targeting techniques has permitted the construction of specific genetic deficiencies to evaluate the biological contribution(s) of an individual protein. Mice lacking a precise DNA repair activity have been generated, and these mutants show various combinations of defective embryogenesis, tissue-specific dysfunction, hypersensitivity to DNA-damaging agents, premature senescence, genetic instability, and elevated cancer rates (1). That repair-deficient animals display such abnormalities underscores the fundamental importance of DNA repair in protecting against the mutagenic and cytotoxic effects of DNA damage.
The Design of Broadside-Coupled Stripline Circuits (1981)

Accurate design expressions for broadside-coupled striplines are presented. The expressions have general validity as long as W/S>0.35. Uncertainty analysis is described to calculate the effect of tolerances in parameters on coupling coefficient and input VSWR. The effect of tolerances in parameters on these characteristics increase as the coupling becomes tighter and tighter.
Ferroelectric PbTiO<sub>3</sub> Thin Films Prepared by Multi-Ion-Beam Sputter and Ion-Assisted Deposition (1993)

Very thin PbTiO 3 films of ∼350 Å were synthesized in situ on MgO substrates by multi-ion-beam sputter deposition using metal targets. Crystalline films in a perovskite phase were obtained at a low substrate temperature of 415°C by means of reactive sputtering in an oxygen ambient. The formation of crystal phases was studied as a function of deposition parameters, and ion-beam-assisted deposition using an electron cyclotron resonance (ECR) plasma was also employed to improve the film growth. Low-energy (<300 eV) oxygen ion bombardment was found to enhance c -axis orientation and crystallization at a lower substrate temperature of 400°C.
Allylic amination of olefins and acetylenes by imido selenium compounds (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAllylic amination of olefins and acetylenes by imido selenium compoundsK. B. Sharpless, Tetsuo Hori, L. K. Truesdale, and C. O. DietrichCite this: J. Am. Chem. Soc. 1976, 98, 1, 269–271Publication Date (Print):January 1, 1976Publication History Published online1 May 2002Published inissue 1 January 1976https://pubs.acs.org/doi/10.1021/ja00417a062https://doi.org/10.1021/ja00417a062research-articleACS PublicationsRequest reuse permissionsArticle Views2412Altmetric-Citations170LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Plasma sterols and depressive symptom severity in a population-based cohort (2017)

Convergent evidence strongly suggests major depressive disorder is heterogeneous in its etiology and clinical characteristics. Depression biomarkers hold potential for identifying etiological subtypes, improving diagnostic accuracy, predicting treatment response, and personalization of treatment. Human plasma contains numerous sterols that have not been systematically studied. Changes in cholesterol concentrations have been implicated in suicide and depression, suggesting plasma sterols may be depression biomarkers. Here, we investigated associations between plasma levels of 34 sterols (measured by mass spectrometry) and scores on the Quick Inventory of Depressive Symptomatology-Self Report (QIDS-SR16) scale in 3117 adult participants in the Dallas Heart Study, an ethnically diverse, population-based cohort. We built a random forest model using feature selection from a pool of 43 variables including demographics, general health indicators, and sterol concentrations. This model comprised 19 variables, 13 of which were sterol concentrations, and explained 15.5% of the variation in depressive symptoms. Desmosterol concentrations below the fifth percentile (1.9 ng/mL, OR 1.9, 95% CI 1.2-2.9) were significantly associated with depressive symptoms of at least moderate severity (QIDS-SR16 score ≥10.5). This is the first study reporting a novel association between plasma concentrations cholesterol precursors and depressive symptom severity.
Production of identical twin and triplet mice by nuclear transplantation (1991)

Transplantation of a single nucleus from two- or four-cell embryos into one of the enucleated blastomeres of a two-cell embryo resulted in successful production of identical triplet and twin mice. The proportion of reconstituted embryos that developed in blastocysts was 71% (84/118) when four-cell embryos were used as donors of nuclei; 10 sets of quadruplet and nine sets each of triplet and twin blastocysts were obtained by this technique. After transfer to recipients, 30% (18/61) developed to term, and one set of identical triplet and four sets of identical twin mice were obtained. When two-cell embryos were used as donors of nuclei, 79 (95%) sets of twin embryos developed to blastocysts. Of 38 twin blastocysts transferred to recipients, 21 sets (55%) developed to term as identical twin mice. These results demonstrate that the enucleated two-cell embryo develops in vitro after transfer of a nucleus from a two- or four-cell embryo and the resultant blastocyst has high potential for development to term after transfer to a recipient.