gebhart/patrepeval huggingface dataset:
Reaction of Human Myoglobin and Nitric Oxide (2001)

The amino acid sequence of human myoglobin (Mb) is similar to other mammalian Mb except for a unique cysteine residue at position 110 (Cys(110)). Anaerobic treatment of ferrous forms of wild-type human Mb, the C110A variant of human Mb or horse heart Mb, with either authentic NO or chemically derived NO in vitro yields heme-NO complexes as detected by electron paramagnetic resonance spectroscopy (EPR). By contrast, no EPR-detectable heme-NO complex was observed from the aerobic reactions of NO and either the ferric or oxy-Mb forms of wild-type human or horse heart myoglobins. Mass analyses of wild-type human Mb treated aerobically with NO indicated a mass increase of approximately 30 atomic mass units (i.e., NO/Mb = 1 mol/mol). Mass analyses of the corresponding apoprotein after heme removal showed that NO was associated with the apoprotein fraction. New electronic maxima were detected at A(333 nm) (epsilon = 3665 +/- 90 mol(-)(1) cm(-)(1); mean +/- S.D.) and A(545 nm) (epsilon = 44 +/- 3 mol(-)(1) cm(-)(1)) in solutions of S-nitrosated wild-type human Mb (similar to S-nitrosoglutathione). Importantly, the sulfhydryl S-H stretch vibration for Cys(110) measured by Fourier transform infrared (nu approximately 2552 cm(-)(1)) was absent for both holo- and apo- forms of the wild-type human protein after aerobic treatment of the protein with NO. Together, these data indicate that the reaction of wild-type human Mb and NO yields either heme-NO or a novel S-nitrosated protein dependent on the oxidation state of the heme iron and the presence or absence of dioxygen.
Three-dimensional Gelatin and Gelatin/Hyaluronan Hydrogel Structures for Traumatic Brain Injury (2006)

Brain tissue engineering has now emerged as one of the most promising treatments for the traumatic brain injury. In this article, two groups of three-dimensional (3D) hydrogel structures composed of gelatin and gelatin/hyaluronan have been formed using our 3D cell assembly technique for in vivo study in rats, in order to investigate their effects in reparation of injury in the central nervous system (CNS). The structures were implanted into cortical defects created in rat brains, and their abilities to improve the brain tissue reconstruction were then evaluated. After 4, 8, 10, and 13 weeks of implantation, sections of brains were processed with NISSL staining for observing the immigration of host neural cells into the implanted materials and the degradation property of the materials. The results showed that simplex gelatin and gelatin/hyaluronan (20:1) with 3D structures both have good biocompatibility with brain tissue while gelatin/hyaluronan has a better contiguity with the surrounding tissue. Through our primary study, it seems that 3D gelatin/hyaluronan structures may be useful in brain tissue repair.
The Smallest and One of the Brightest. Efficient Preparation and Optical Description of the Parent Borondipyrromethene System (2009)

Fully unsubstituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) 1 was prepared. Reaction of 8-thiomethylbodipy 2 with triethylsilane in the presence of a catalytic amount of Pd and a stoichiometric amount of copper(I) thienyl-2-carboxylate (CuTC) in THF at 55 degrees C gave compound 1 in nearly quantitative yield. This compound displays high quantum yields (up to 93%) in polar solvents including water. Its optical properties and crystal structure are discussed.
BEN/SC1/DM-GRASP, a homophilic adhesion molecule, is required for in vitro myeloid colony formation by avian hemopoietic progenitors. (1996)

BEN/SC1/DM-GRASP is a membrane glycoprotein of the immunoglobulin superfamily isolated in the chick by several groups, including ours. Its expression is strictly developmentally regulated in several cell types of the nervous and hemopoietic systems and in certain epithelia. Each of these cell types expresses isoforms of BEN which differ by their level of N-glycosylation and by the presence or absence of the HNK-1 carbohydrate epitope. In the present work, the influence of glycosylation on BEN homophilic binding properties was investigated by two in vitro assays. First, each BEN isoform was covalently coupled to microspheres carrying different fluorescent dyes and an aggregation test was performed. We found that homophilic aggregates form indifferently between the same or different BEN isoforms, showing that glycosylation does not affect BEN homophilic binding properties. This was confirmed in the second test, where the BEN-coated microspheres bound to the neurites of BEN- expressing neurons, irrespective of the isoform considered. The transient expression of the BEN antigen on hemopoietic progenitors prompted us to see whether it might play a role in their proliferation and differentiation. When added to hemopoietic progenitor cells in an in vitro colony formation assay anti-BEN immunoglobulin strongly inhibited myeloid, but not erythroid, colony formation although both types of precursors express the molecule.
Adaptive hysteresis band control for DC-DC buck converter (2013)

This paper presents an improved adaptive hysteresis band control (AHBC) technique for DC-DC buck converter, which can adaptively adjust the hysteresis band based on input and output voltages to achieve fast dynamic response and good steady state. Since the proposed control scheme only uses a comparator with hysteresis and a feedback resistor, numbers of components in a control circuit are reduced. Also, error amplifier is not used, the stability of the converter will be become excellent. Finally, steady state analysis of the proposed converter is performed and verified by simulation results.
[Formulation of a rice-based beverage of high nutritive value]. (1981)

The purpose of this work was the production of a beverage with a nutritive value similar to that of milk, but available at a lower cost. A traditional beverage in Venezuela, "Chicha de Arroz" (Rice Chicha), was chosen. The various formulas studied were based on rice, non-fat milk and different kinds of soy flour, in addition to sugar, vegetable oil, vitamins, ferrous sulfate and different flavors. A product with protein and caloric values similar to those of milk was obtained. Twenty formulas were prepared and submitted to physico-chemical, microbiological and sensorial evaluations with respect to flavor, color, viscosity and stability during period of refrigeration. The most adequate formula was prepared on a pilot-plant scale. Rat assays gave the same PER results as those of casein. Consumer acceptability was tested on 1,080 school children, and showed to be greater than 95%. The stability was superior to that of milk and the cost, approximately three-fourths that of the latter. In the school-snack program of the National Institute of Nutrition, milk is now being replaced by this Chicha.
P02.12: Bladder outlet obstruction: is it effective using a new‐typed catheter in vesicoamniotic shunt? (2004)

With the natural course of many fetal bladder outlet obstructions being prognostically poor, fetal intervention is the accepted procedure in selected cases. The purpose of this study was to investigate the efficacy of vesicoamniotic shunt using a new-typed catheter in terms of reducing perinatal mortality and morbidity. This retrospective study included 7 prenatally diagnosed bladder outlet obstructions from 1998 to 2004. We performed vesicoamniotic shunts using a basket-shaped catheter and patients were followed up for prenatal and neonatal outcome analyses. We underwent 7 vesicoamniotic shunts between 13.7 and 29.3 weeks of gestations (mean ± SD, 21.5 ± 5.0). Final diagnoses included 5 posterior urethral valves, 1 cloacal anomaly, and 1 urethral stenosis. There was no maternal complication related to the procedure. Mean gestational age at delivery was 33.9 ± 3.1 weeks. Shunt dislodgements occurred in 3 cases, all of which were found after 32 weeks of gestation. One woman diagnosed as having a fetus with a posterior urethral valve decided to terminate her pregnancy. Six women delivered live babies, none of whom was required to have postnatal ventilatory support. Duration of postnatal follow up ranged from 1 to 47 months. Of the six newborns, five survived with normal renal functions and 1 died of renal failure at 3 months of age. Vesicoamniotic shunt using a basket-shaped catheter may be effective in improving perinatal and neonatal outcomes through long-term decompression of the fetal urinary tract in carefully selected cases.
The Changing Use of Pneumococcal Vaccine in Developed Countries (1999)

This article has no abstract.
Human Recombinant Phosphodiesterase 4B2B Binds (<i>R</i>)-Rolipram at a Single Site with Two Affinities (1997)

The interactions between (R)-rolipram and purified human recombinant low-Km, cAMP-specific phosphodiesterase (HSPDE4B2B) constructs were investigated using biochemical, kinetic, and biophysical approaches. The full-length protein (amino acids 1-564) and an N-terminal truncated protein (amino acids 81-564) exhibited high-affinity (R)-rolipram binding, whereas an N-terminal and C-terminal truncated protein (amino acids 152-528) lacked high-affinity (R)-rolipram binding. The 152-528 and 81-564 proteins had similar Km's and kcat/Km's and differed less than 4-fold compared with the 1-564 protein. (R)-Rolipram inhibition plots were biphasic for the 1-564 and 81-564 proteins and fit to two states, a high-affinity (Ki = 5-10 nM) state and a low-affinity (Ki = 200-400 nM) state, whereas the 152-528 protein fit to a single state (Ki = 350-400 nM). The stoichiometry for high-affinity binding using a filter binding assay was found to be <1 mol of (R)-rolipram per mole of 1-564 or 81-564 protein. Titration microcalorimetric studies revealed both a high-affinity state with a stoichiometry of 0.3 mol of (R)-rolipram per mole of protein and a low-affinity state with a stoichiometry of 0.6 mol of (R)-rolipram per mole of protein for the 81-564 protein. A single low-affinity state with a stoichiometry of 0.9 mol of (R)-rolipram per mole of protein was seen using the 152-528 protein. The data indicate that purified HSPDE4B2B 1-564 and 81-564 proteins contain a single binding site for (R)-rolipram and suggest that the proteins exist in two different states distinguishable by their affinity for (R)-rolipram. Furthermore, the high-affinity binding state of the protein requires amino acid residues at the N-terminus (81-151) of the protein and catalytic domain (152-528), whereas the low-affinity binding state only requires residues in the catalytic domain (152-528). Phosphorylation at residues 487 and 489 of the 81-564 protein does not appear to alter the substrate kinetics or the stoichiometry and binding affinity of (R)-rolipram.
Photo-Activated Psoralen Binds the ErbB2 Catalytic Kinase Domain, Blocking ErbB2 Signaling and Triggering Tumor Cell Apoptosis (2014)

Photo-activation of psoralen with UVA irradiation, referred to as PUVA, is used in the treatment of proliferative skin disorders. The anti-proliferative effects of PUVA have been largely attributed to psoralen intercalation of DNA, which upon UV treatment, triggers the formation of interstrand DNA crosslinks (ICL) that inhibit transcription and DNA replication. Here, we show that PUVA exerts antitumor effects in models of human breast cancer that overexpress the ErbB2 receptor tyrosine kinase oncogene, through a new mechanism. Independent of ICL formation, the antitumor effects of PUVA in ErbB2+ breast cancer models can instead be mediated through inhibition of ErbB2 activation and signaling. Using a mass spectroscopy-based approach, we show for the first time that photo-activated 8MOP (8-methoxypsoralen) interacts with the ErbB2 catalytic autokinase domain. Furthermore, PUVA can reverse therapeutic resistance to lapatinib and other ErbB2 targeted therapies, including resistance mediated via expression of a phosphorylated, truncated form of ErbB2 (p85(ErbB2)) that is preferentially expressed in tumor cell nuclei. Current ErbB2 targeted therapies, small molecule kinase inhibitors or antibodies, do not block the phosphorylated, activated state of p85(ErbB2). Here we show that PUVA reduced p85(ErbB2) phosphorylation leading to tumor cell apoptosis. Thus, in addition to its effects on DNA and the formation of ICL, PUVA represents a novel ErbB2 targeted therapy for the treatment of ErbB2+ breast cancers, including those that have developed resistance to other ErbB2 targeted therapies.
Opposite Spectrum of Activity of Canonical Wnt Signaling in the Osteogenic Context of Undifferentiated and Differentiated Mesenchymal Cells: Implications for Tissue Engineering (2010)

To delineate the competence window in which canonical wingless (Wnt)-signaling can either inhibit or promote osteogenic differentiation, we have analyzed cells with different status, specifically undifferentiated mesenchymal cells, such as adipose-derived stem cells and embryonic calvarial mesenchymal cells, and differentiated mesenchymal cells such as juvenile immature calvarial osteoblasts and adult calvarial osteoblasts. Our analysis indicated that undifferentiated mesenchymal cells and juvenile calvarial osteoblasts are endowed with higher levels of endogenous canonical Wnt signaling compared to fully differentiated adult calvarial osteoblasts, and that different levels of activation inversely correlated with expression levels of several Wnt antagonists. We have observed that activation of canonical Wnt signaling may elicit opposite biological activity in the context of osteogenic differentiation depending on the status of cell, the threshold levels of its activation, and Wnt ligands concentration. The results presented in this study indicate that treatment with Wnt3 and/or expression of constitutively activated β-catenin inhibits osteogenic differentiation of undifferentiated mesenchymal cells, whereas expression of dominant negative transcription factor 4 (Tcf4) and/or secreted frizzled related protein 1 treatment enhances their osteogenic differentiation. Wnt3a treatment also inhibits osteogenesis in juvenile calvarial osteoblasts in a dose-dependent fashion. Conversely, Wnt3a treatment strongly induces osteogenesis in mature calvarial osteoblasts in a dose-dependent manner. Importantly, in vitro data correlated with in vivo results showing that Wnt3a treatment of calvarial defects, created in juvenile mice, promotes calvarial healing and bone regeneration only at low doses, whereas high doses of Wnt3a impairs tissue regeneration. In contrast, high doses of Wnt3a enhance bony tissue regeneration and calvarial healing in adult mice. Therefore, the knowledge of both endogenous activity of canonical Wnt signaling and appropriate concentrations of Wnt3a treatment may lead to significant improvement for bony tissue engineering, as well as for the efficient implement of adipose-derived stem cells in bone regeneration. Indeed, this study has important potential implications for tissue engineering, specifically for repair of juvenile bone defects.
Cellular strategies for making monoubiquitin signals (2011)

Post-translational modification of proteins with ubiquitin regulates a variety of eukaryotic cellular processes. Ubiquitin can be conjugated to substrates either as a single moiety (monoubiquitination) or as isopeptide bond-linked chains (polyubiquitination), creating an array of ubiquitin signals. It has been established that monoubiquitination can serve important functions in many biological processes such as the regulation of gene transcription, protein trafficking, and DNA repair. Surprisingly, little is known about the mechanisms by which monoubiquitin signals are produced in the cell. Here, we discuss the potential cellular strategies for generating monoubiquitinated proteins using a few, relatively well characterized examples of monoubiquitinated proteins. These strategies include coupling ubiquitination to low affinity ubiquitin binding, using monoubiquitination-dedicated E2 conjugating enzymes, and restricting ubiquitin chain elongation. Some of these principles may be applicable to protein modifications involving ubiquitin like proteins (UBLs), which often occur in monomeric form.
(Invited) Electrolytes, SEI and Charge Discharge Kinetics in Li-Ion Batteries (2010)

Charge discharge kinetics of Li-ion batteries is dominated by the lithium ion (Li+) charge transfer kinetics, which involves the process of transporting the solvated Li+ in the electrolyte to the insertion of Li+ and the accepting of an electron at the same time in the electrode active materials. The importance of electrolytes and recent studies of Li+ charge transfer kinetics were briefly reviewed. Using 3-electrode cells and a DC Pulse Current Impedance method, we examined the charge discharge kinetics of the anode and the cathode in the same electrolyte at the same time. We observed a slower kinetics at the graphitic carbon anode as indicated by higher activation energy than that at the lithium nickel cobalt aluminum mixed oxide (LiNi0.80Co0.15Al0.05O2) cathode. While desolvation is a dominating step as concluded in recent studies on the Li+ charge transfer kinetics, this study suggests that the nature of SEIs and electrode materials play crucial roles on Li+ charge discharge kinetics.
Action Recognition in Video by Covariance Matching of Silhouette Tunnels (2009)

Action recognition is a challenging problem in video analytics due to event complexity, variations in imaging conditions, and intra- and inter-individual action-variability. Central to these challenges is the way one models actions in video, i.e., action representation. In this paper, an action is viewed as a temporal sequence of local shape-deformations of centroid-centered object silhouettes, i.e., the shape of the centroid-centered object silhouette tunnel. Each action is represented by the empirical covariance matrix of a set of 13-dimensional normalized geometric feature vectors that capture the shape of the silhouette tunnel. The similarity of two actions is measured in terms of a Riemannian metric between their covariance matrices. The silhouette tunnel of a test video is broken into short overlapping segments and each segment is classified using a dictionary of labeled action covariance matrices and the nearest neighbor rule. On a database of 90 short video sequences this attains a correct classification rate of 97%, which is very close to the state-of-the-art, at almost 5-fold reduced computational cost. Majority-vote fusion of segment decisions achieves 100% classification rate.
Neural cell adhesion molecule expression in plasma cells in bone marrow biopsies and aspirates allows discrimination between multiple myeloma, monoclonal gammopathy of uncertain significance and polyclonal plasmacytosis (2004)

We conclude that CD56 antibody is a very useful marker in the study of plasma cell proliferation in bone marrow biopsies and in bone marrow aspirates and is a great help in discriminating between MM, MGUS, and polyclonal plasmacytosis, especially in those cases with low infiltration.
A Nitride-Isolated Molybdenum-Polysilicon Gate Electrode (1985)

A low resistive MTP gate electrode has been developed for MOS VLSIs. The MTP gate features are as follows: 1) The resistivity of the electrode is reduced by about two orders of magnitude below that of poly Si even after annealing at 1100°C.
Synthesis and magnetic resonance studies of some paramagnetic transition metal aminotroponiminates (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and magnetic resonance studies of some paramagnetic transition metal aminotroponiminatesD. R. Eaton, W. R. McClellan, and J. F. WeiherCite this: Inorg. Chem. 1968, 7, 10, 2040–2046Publication Date (Print):October 1, 1968Publication History Published online1 May 2002Published inissue 1 October 1968https://pubs.acs.org/doi/10.1021/ic50068a016https://doi.org/10.1021/ic50068a016research-articleACS PublicationsRequest reuse permissionsArticle Views152Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Functional Analysis of the Acquisition and Maintenance of Janitorial Skills in a Competitive Work Setting (1988)

There is a need for empirically based studies to support public school on-the-job training programs for students with severe disabilities. This study examined the use of supported employment via the supported jobs model to provide competitive work experience for a 19-year-old student with severe disabilities. The job trained involved a complex set of janitorial skills, including emptying trash cans, detail cleaning, and daily cleaning. Training consisted of a combination of total task presentation and an individualized prompting hierarchy. A multiple baseline across behaviors design was employed across the three sets of behaviors. Results demonstrated the acquisition and maintenance of all skills at 100% accuracy. The use of supported employment as a means of providing competitive work experience for students with severe disabilities and the need for further research on effective community-based vocational training strategies are discussed.
Silicon tetrachloride as a coupling reagent for amide formation (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSilicon tetrachloride as a coupling reagent for amide formationTak-Hang Chan and L. T. L. WongCite this: J. Org. Chem. 1969, 34, 9, 2766–2767Publication Date (Print):September 1, 1969Publication History Published online1 May 2002Published inissue 1 September 1969https://pubs.acs.org/doi/10.1021/jo01261a064https://doi.org/10.1021/jo01261a064research-articleACS PublicationsRequest reuse permissionsArticle Views584Altmetric-Citations58LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts