gebhart/patrepeval huggingface dataset:
Method for selective quantification of adipose-derived stromal/stem cells in tissue (2016)

Fat grafts are valuable for soft-tissue regeneration and augmentation. However, fat graft systems require further improvement for the prediction of graft retention. The concentration of adipose-derived stromal/stem cells (ASCs) is one of the most important factors that affect graft retention; however, current cell quantification techniques have not been applied to adipose tissue. Here we developed a method for the selective quantification of ASCs in tissue (SQAT). We identified a characteristic methylated site in the CD31 promoter after searching for specific markers of ASCs. This DNA methylation was not detected in any cell type other than ASCs in adipose tissue. Therefore, analyzing this methylation may be a suitable approach for quantifying ASCs in tissues because DNA is readily extracted from tissues. SQAT is based on quantifying this methylation by quantitative polymerase chain reaction using methylation-sensitive HapII-treated DNA as the template. SQAT was validated based on the numbers of ASCs determined by CD31<sup>-</sup>/CD34<sup>+</sup>-based flow cytometry. The results obtained by both methods were perfectly correlated, thereby demonstrating that SQAT is a useful tool for quantifying ASCs. SQAT analysis using ASCs isolated from suctioned fat according to the standard protocol (<i>i.e.</i>, collagenase treatment) showed that the yield of ASCs was 59% ± 21%, which suggests that the ASC isolation technique requires further improvement. Furthermore, SQAT is an excellent method for quantifying ASCs in arbitrary samples (particularly tissue), which could dramatically improve ASC isolation technologies and fat graft systems, thereby facilitating the prediction of graft retention.
Some Methods of Labeling Nature Trails (1934)

For several years the students of botany of Iowa Wesleyan College have maintained a nature trail in the Oakland Mills State Park. The writer is one of the three students appointed to take care of this work for the current year. When the trails were first inaugurated it was necessary to select the course, lay out the path and erect signs to keep the visitors going in the right direction, as well as to label items of interest. Now through the activity of the C.C.C. workers, permanently constructed paths run through the highly interesting parts of the park and one needs only to locate and label the more attractive items along some of these paths.
SPR sensor signal amplification based on dye-doped polymer particles (2006)

A novel amplification method was prepared based on dye-doped polymer particles for SPR signals originating from antigen–antibody (BSA–anti-BSA) interactions. Coloring the particles enhances the change of SPR signals based on the change of the imaginary part of the refractive index. The signal amplification effect in the reflectance mode was 31-fold stronger compared to the effect obtained with white latex particles and the combined amplification effect due to the presence of polymer particles and the colorant was over 100-fold compared to non-amplified SPR signals originating from BSA–anti-BSA interactions. The amplification method based on the dye-doped particles is widely applicable for the analysis of antibody–antigen interactions and DNA interactions at low concentrations.
The Eukaryotic Genome as an RNA Machine (2008)

The past few years have revealed that the genomes of all studied eukaryotes are almost entirely transcribed, generating an enormous number of non-protein-coding RNAs (ncRNAs). In parallel, it is increasingly evident that many of these RNAs have regulatory functions. Here, we highlight recent advances that illustrate the diversity of ncRNA control of genome dynamics, cell biology, and developmental programming.
Modeling Self-Assembly Processes Driven by Nonbonded Interactions in Soft Materials (2008)

This Centennial Feature Article provides an overview of research in the general area of self-assembly modeling, with particular emphasis on the self-assembly of molecules into soft nanoscale structures where the driving force for assembly is provided by nonbonded interactions (hydrogen bonds and electrostatics). The models have been developed at many different levels of theory, going all the way from simple analytical models of packing effects to atomistic descriptions using molecular dynamics methods. In between these limits are mean-field and coarse-grained models, including models for DNA, peptides, and lipids that can be used to describe the assembly of hybrid (amphiphilic) materials. Several recent applications to specific systems are discussed, including the description of peptide amphiphile assembly to make cylindrical micelles, the assembly and melting of DNA hairpins, the use of DNA tethers to assemble nanoparticles into aggregates and crystalline structures, and the use of coarse-grained lipid models to make lamellar and high-curvature phases. These examples demonstrate the difficulties associated with brute force atomistic methods, and they also show the opportunities (but uncertainties and ambiguities) associated with simpler models such as coarse-grained models. The examples also demonstrate the usefulness of successful modeling methods in the design of new materials, including an understanding of the relationship between structure and function.
Suppressing inflammation by inhibiting the NF‐κB pathway contributes to the neuroprotective effect of angiotensin‐(1‐7) in rats with permanent cerebral ischaemia (2012)

Our findings indicate that suppressing NF-κB dependent pathway via Mas receptor may represent one mechanism that contributes to the anti-inflammatory effects of Ang-(1-7) in rats with pMCAO.
Hydrogel optimization for cultured elastic chondrocytes seeded onto a polyglycolic acid scaffold* (2005)

The purpose of this study was to compare the effect of different hydrogels on the production of tissue-engineered cartilage based on polyglycolic acid (PGA). Chondrocytes were isolated from adult sheep auricles. Alginate, Type I collagen, methylcellulose, and pluronic F127 hydrogels were evaluated, as were controls prepared without hydrogels. Proliferated chondrocytes were mixed with each hydrogel at 20 x 10(6) cells/mL and seeded onto PGA (1 x 1 x 0.2 cm, n = 60). The constructs were cultured with serum-free medium containing 5 ng/mL TGF-beta(2) and 5 ng/mL des(1-3)IGF-I in rotational bioreactors for up to 6 weeks. The cellular morphology, histology, and biochemistry were analyzed. Type I collagen, methylcellulose, and pluronic F127 displayed improved cartilage matrix deposition in terms of histology and biochemistry compared to alginate. It was not concluded that the combined seeding of chondrocytes and hydrogels on a PGA scaffold had significantly better effects than cell seeding without hydrogels. However, the histology and other useful findings in this ECM analyses suggested that Type I collagen and MC hydrogels were the best candidates for cartilage regeneration, because of their stimulation for chondrocyte proliferation in a three-dimensional culture as well as cartilage regeneration.
Development of contraceptive vaccines for humans using antigens derived from gametes (spermatozoa and zona pellucida) and hormones (human chorionic gonadotrophin): current status (1995)

Contraceptive research has entered a new phase of development with the advent of hybridoma and DNA recombinant technologies. During the past 5 years, significant advances have been made in this area and now it seems that realistic prospects exist for the development of contraceptive vaccines for use in humans and animals (veterinary, wild and domestic), applicable to both the female and male sexes. Contraceptive vaccines will be valuable supplements to the presently available methods of family planning, and, due to high specificity, the occurrence of limited side-effects if any, low cost and infrequent administration, contraceptive vaccines may have greater acceptability than the currently available methods. Mammalian reproduction starts with the unison of gametes contributed by the male and female partners. Both spermatozoon and oocyte have antigens on the cell surface that are unique, tissue-specific, immunogenic and accessible to antibodies, and binding of the antibodies to these antigens can cause inhibition of gamete function, resulting in a failure of fertilization. Fertilization is followed by embryogenesis, with the early embryo producing several proteins, some of which, e.g. human chorionic gonadotrophin (HCG), have a vital role in the establishment and maintenance of early pregnancy. Again, these proteins are accessible to antibodies, and their immunoneutralization can cause anti-fertility effects with loss of early embryo. Thus, the antigens derived from proteins on spermatozoa, oocyte and early embryo, especially HCG, constitute interesting molecules for the development of contraceptive vaccines. The aim of the present article is to review the current status of development of contraceptive vaccines based on antigens derived from sperm cell, oocyte zona pellucida and HCG, and to discuss their relative merits and future development.
TMSTAF The Extended Use of STAF on Test Automation (2012)

As software packages becomes increasingly large and complex, the time required for testing them, throughout the development lifecycle, has also increased. Because testing activities consume the majority of software Quality Assurance (QA) resources, a test platform was needed to speed up test cycles without any decrease in test result accuracy. The use of Python 2.6 scripting language to create a faster, automated testing platform is reported here. Trend Micro Software Testing Automation Framework (TMSTAF) was developed using Python 2.6, based on Software Testing Automation Framework (STAF), to improve automated testing. We found that TMSTAF not only decreased testing time, it provided faster process integration, test feedback, and improved overall software quality. Using the TMSTAF automation environment for development and execution is simple to set up. Test cases can be created for use in both manual and automated tasks, converted to automated scripts, and implemented with structural and flexible mechanisms. Automation script pre-runs and debugging make troubleshooting more efficiently; TMSTAF test report data can be used to identify quality issues quickly. TMSTAF can be seamlessly integrated into the build release process, making it a smart option for software QA engineers.
Thin Films of Highly Luminescent Lanthanide Complexes Covalently Linked to an Organic−Inorganic Hybrid Material via 2-Substituted Imidazo[4,5-<i>f</i>]-1,10-phenanthroline Groups (2005)

A homogeneous distribution of luminescent lanthanide(III) 2-thenoyltrifluoroacetonate complexes (Ln = Pr, Nd, Sm, Eu, Dy, Ho, Er, Tm, Yb) in an organic−inorganic hybrid material was obtained by grafting the complexes to the solid matrix via 2-substituted imidazo[4,5-f]-1,10-phenanthroline moieties. Thin films of the silica hybrid material were prepared by the sol−gel method and by spin-coating of the gelating solution on glass slides or on oxidized silicon wafers. The thickness of the thin films was determined by scanning electron microscopy (SEM). High-resolution luminescence spectra of the lanthanide complexes were recorded, and the luminescence decay times were measured. The luminescence quantum yields of europium(III)-doped thin films exposed to different drying conditions were determined by using an integrating sphere.
Binary and multi-valued SPFD-based wire removal in PLA networks (2002)

This paper describes the application of binary and multivalued SPFD-based wire removal techniques for circuit implementations utilizing networks of PLAs. It has been shown that a design style based on a multi-level network of approximately equal-sized PLAs results in a dense, fast, and crosstalk-resistant layout. Wire removal is a technique where the total number of wires between individual circuit nodes is reduced, either by removing wires, or replacing them with other existing wires. Three separate wire removal experiments are performed. Either wire removal is invoked before clustering the original netlist into a network of PLAs, or after clustering, or both before and after clustering. For wire removal before clustering, binary SPFD-based wire removal is used. For wire removal after clustering, multi-valued SPFD-based wire removal is used since the multi-output PLAs can be viewed as multi-valued single output nodes. We demonstrate that these techniques are effective. The most effective approach is to perform wire removal both before and after clustering. Using these techniques, we obtain a reduction in placed and routed circuit area of about 11%. This reduction is significantly higher (about 20%) for the larger circuits we used in our experiments.
Quantum Computing by an Optimal Control Algorithm for Unitary Transformations (2002)

Quantum computation is based on implementing selected unitary transformations representing algorithms. A generalized optimal control theory is used to find the driving field that generates a prespecified unitary transformation. The approach is independent of the physical implementation of the quantum computer and it is illustrated for one and two qubit gates in model molecular systems, where only part of the Hilbert space is used for computation.
Four-channel Ti:LiNbO_3 wavelength division multiplexer for 13-μm wavelength operation (1991)

A four-channel Ti:LiNbO(3) wavelength division multiplexer (WDM) that uses cascaded two-mode-interference dual-channel WDM waveguide structures was demonstrated. Its implementation in integrated-optic form affords ease of fabrication, voltage tunability, and the potential for combining other devices for different functions on the same chip. The device was operated in the wavelength region of 1.3 microm. The channel spacings ranged from 12 to 15 nm. The channel wavelengths can be electro-optically tuned at the rate of 1 nm/V. The cross talk was measured to be less than -20 dB.
Performance of Industrial Equipment to Collect Coolant Mist (1996)

Abstract A protocol was developed to evaluate collector performance in the laboratory under conditions that simulate plant practice. Eight collector manufacturers provided nine samples of their products, each designed to handle 1700 m3/hr (1000 ft3/min) of airflow. Each collector was tested using mists of mineral oil, soluble oil, and synthetic fluid; for a three-stage collector, tests with each metalworking fluid lasted for thirteen 24-hour days. For each collector stage as well as for each assembled collector, tests established the relationship between efficiency and droplet diameter for droplets from less than 0.3 to about 6 µm in diameter. Substantial differences in efficiency and pressure drop were found among the stages and assembled collectors. Some metal mesh filters worked well as first stages, whereas pocket filters, one cartridge filter, and an electrostatic precipitator worked well as second stages. High-efficiency particulate air (HEPA), 95% dioctyl phthalate (DOP), and candle filters provided excellent efficiency as final stages for droplets of all sizes. When collecting mineral oil mist, the efficiency of some first and second stages deteriorated substantially over the relatively short term of these tests. Keywords: collectormistperformance
Guaiacol as a drug candidate for treating adult polyglucosan body disease (2018)

Adult polyglucosan body disease (APBD) is a late-onset disease caused by intracellular accumulation of polyglucosan bodies, formed due to glycogen-branching enzyme (GBE) deficiency. To find a treatment for APBD, we screened 1,700 FDA-approved compounds in fibroblasts derived from APBD-modeling GBE1-knockin mice. Capitalizing on fluorescent periodic acid-Schiff reagent, which interacts with polyglucosans in the cell, this screen discovered that the flavoring agent guaiacol can lower polyglucosans, a result also confirmed in APBD patient fibroblasts. Biochemical assays showed that guaiacol lowers basal and glucose 6-phosphate-stimulated glycogen synthase (GYS) activity. Guaiacol also increased inactivating GYS1 phosphorylation and phosphorylation of the master activator of catabolism, AMP-dependent protein kinase. Guaiacol treatment in the APBD mouse model rescued grip strength and shorter lifespan. These treatments had no adverse effects except making the mice slightly hyperglycemic, possibly due to the reduced liver glycogen levels. In addition, treatment corrected penile prolapse in aged GBE1-knockin mice. Guaiacol's curative effects can be explained by its reduction of polyglucosans in peripheral nerve, liver, and heart, despite a short half-life of up to 60 minutes in most tissues. Our results form the basis to use guaiacol as a treatment and prepare for the clinical trials in APBD.
Reduced graphene oxide/carbon double-coated 3-D porous ZnO aggregates as high-performance Li-ion anode materials (2015)

The reduced graphene oxide (RGO)/carbon double-coated 3-D porous ZnO aggregates (RGO/C/ZnO) have been successfully synthesized as anode materials for Li-ion batteries with excellent cyclability and rate capability. The mesoporous ZnO aggregates prepared by a simple solvothermal method are sequentially modified through distinct carbon-based double coating. These novel architectures take unique advantages of mesopores acting as space to accommodate volume expansion during cycling, while the conformal carbon layer on each nanoparticle buffering volume changes, and conductive RGO sheets connect the aggregates to each other. Consequently, the RGO/C/ZnO exhibits superior electrochemical performance, including remarkably prolonged cycle life and excellent rate capability. Such improved performance of RGO/C/ZnO may be attributed to synergistic effects of both the 3-D porous nanostructures and RGO/C double coating.
THE SURVIVAL OF MAMMALIAN TISSUES PERFUSED WITH INTRAVASCULAR GAS MIXTURES OF OXYGEN AND CARBON DIOXIDE (1958)

It is possible to maintain active mammalian cardiac and skeletal muscle in good condition for 3 or 4 hours by the intravascular perfusion of these tissues with warm, moist oxygen containing 5% carbon dioxide. Some of the uses of this perfusion technique are discussed.
A Structural Resolution Cryo-TEM Study of the Early Stages of MFI Growth (2008)

Room-temperature aging of zeolite precursor silica sol was followed by SAXS and cryo-TEM. Cryo-TEM imaging of zeolite materials with structural resolution is demonstrated. The results suggest the formation of predominantly amorphous aggregates before MFI crystallization.
Stabilities of Bivalent Metal Complexes of Some B-Ketoimines (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStabilities of Bivalent Metal Complexes of Some B-KetoiminesDean F. Martin, Gaile A. Janusonis, and Barbara B. MartinCite this: J. Am. Chem. Soc. 1961, 83, 1, 73–75Publication Date (Print):January 1, 1961Publication History Published online1 May 2002Published inissue 1 January 1961https://pubs.acs.org/doi/10.1021/ja01462a015https://doi.org/10.1021/ja01462a015research-articleACS PublicationsRequest reuse permissionsArticle Views705Altmetric-Citations110LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Effects of a New Selective Estrogen Receptor Modulator (MDL 103,323) on Cancellous and Cortical Bone in Ovariectomized Ewes: A Biochemical, Histomorphometric, and Densitometric Study (2001)

The aims of this study performed in ewes were: (1) to confirm in this animal model the effects on bone of ovariectomy (OVX) alone or associated with Lentaron (L), a potent peripheral aromatase inhibitor, used to amplify the effects of OVX and (2) to evaluate the effects of a new selective estrogen receptor modulator (SERM; MDL 103,323) on bone remodeling. Thirty-nine old ewes were divided into five groups: sham (n = 7); OVX (n = 8); OVX + L (n = 8); OVX + L + MDL; 0.1 mg/kg per day (n = 8); and OVX + L + MDL 1 mg/kg per day (n = 8). The animals were treated for 6 months. Biochemical markers of bone turnover (urinary excretion of type 1 collagen C-telopeptide [CTX], serum osteocalcin [OC], and bone alkaline phosphatase [BAP]) were measured each month. Bone biopsy specimens were taken at the beginning and after death at the end of the experiment. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA) on the lumbar spine and femur. OVX induced a significant increase in biochemical markers. This effect was the highest after 3 months for CTX (+156% vs. sham) and after 4 months for OC and BAP (+74% and +53% vs. sham, respectively). L tended to amplify the effect of OVX on OC and BAP. OVX induced significant increases in the porosity, eroded, and osteoid surfaces in cortical bone but no effect was observed in cancellous bone. MDL treatment reduced the bone turnover as assessed by bone markers, which returned to sham levels as well as histomorphometry both in cortical and in cancellous bone. Cancellous osteoid thickness decreased by 27% (p < 0.05), mineralizing perimeter by 81% (p < 0.05), and activation frequency by 84% (p < 0.02) versus OVX + L. Femoral and spinal BMD were increased by MDL and tended to return to the sham values. The effects of OVX on bone turnover were different on cortical and cancellous bone. These effects on cortical bone were reflected by changes in biochemical markers. MDL markedly reduces bone turnover and increases BMD suggesting that this new agent may prevent postmenopausal bone loss.
Generating and exploring good building layouts (2013)

Good building layouts are required to conform to regulatory guidelines, while meeting certain quality measures. While different methods can sample the space of such good layouts, there exists little support for a user to understand and systematically explore the samples. Starting from a discrete set of good layouts, we analytically characterize the local shape space of good layouts around each initial layout, compactly encode these spaces, and link them to support transitions across the different local spaces. We represent such transitions in the form of a portal graph. The user can then use the portal graph, along with the family of local shape spaces, to globally and locally explore the space of good building layouts. We use our framework on a variety of different test scenarios to showcase an intuitive design, navigation, and exploration interface.
Swift XRT/UVOT follow-up of the Black Hole Candidate MAXI J1659-152 during a low luminosity state (2011)

We report on a Swift follow-up observation of the X-ray transient MAXI J1659-152 (ATel #2873, ATel #2877, GCN #11296). This source is suggested to be a stellar-mass black hole candidate (Kalamkar et al. 2011, arXiv:1012.4330). The source also has a very short orbital period ~2.4 hours (Kuulkers et al. 2011, arXiv:1102.2102). The optical counterpart of MAXI J1659-152 has been identified (GCN #11298, ATel #2884), and the quiescent optical magnitude has been found to be 22.4 in r-band (ATel #2976).