gebhart/patrepeval huggingface dataset:
Effect of an anticholinesterase compound on the ultrastructure and function of the rat blood-brain barrier: a review and experiment. (1991)

Soman, an organophosphorous irreversible inhibitor of acetylcholinesterase, was studied for its effect on the rat blood-brain barrier (BBB) during the first 24 h of intoxication. Young adult male Sprague-Dawley rats, injected with Evans blue-dye and surviving a subsequent single convulsive dose of soman (114 micrograms/kg, 0.9LD50), presented focal and diffuse penetration of dye in areas of brain normally considered protected by the BBB. Invasion was widest during the first hour when signs of excitation, respiratory distress and convulsions peaked and was absent at 24 h. During this time period, cholinesterase inhibition, as measured by enzyme assay, persisted in brain and blood at 10% and 6% of control values respectively. Brains of nonconvulsing animals and animals pretreated with nembutal (45 mg/kg, I.P.) or with diazepam (10 mg/kg, I.P.) were free of extravasated dye. A ranking of dye-breached brain areas suggested that cerebellar and cerebral cortex were most frequently involved while brain stem was rarely stained. Ultrastructural analysis of breached areas with horseradish peroxidase as a tracer molecule, revealed that the probable subcellular mechanism of the induced breach was enhanced vesicular transport, a mechanism similarly described for seizure. Consequences of the breach were emphasized with the detection of significantly elevated levels of an exogenously administered quaternary compound, 3H-hexamethonium. These findings present additional evidence that an anticholinesterase-induced breach of the rat blood-brain barrier is convulsive dependent, demonstrates BBB mechanisms similar to that of seizure, and can allow CNS penetration of blood-borne drugs and circulatory proteins that normally would be slowed or excluded by an intact BBB.(ABSTRACT TRUNCATED AT 250 WORDS)
Effect of Ni metallization on interfacial reactions and die attach properties of Zn-Al-Mg-Ga high temperature lead-free solder (2010)

Interfacial reactions during Si die attachment with Zn-Al-Mg-Ga high-temperature lead (Pb)-free solder on bare Cu lead-frame (Tamac 4) and Ni metallized Cu lead-frame were investigated using an optical microscope, scanning electron microscope (SEM) and energy dispersive x-ray (EDX). Die attachment was performed in an automatic die attach machine in a forming gas environment at temperature 380°C. The back side of the die was metallized with Ti/Ni/Ag layers. Comparative studies of die attach properties such as wetting, void and die shear strength on bare and Ni metallized Cu lead-frame was made. Cross sectional microstructural investigation revealed that as many as three intermetallic compound (IMCs) layers form at the solder/lead-frame interface for bare Cu lead-frame. A CuZn intermetallic layer forms close to copper, a scallop shaped CuZn <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> forms at the solder side, while Cu <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">5</sub> Zn <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">8</sub> forms at the middle. At the interface with Si die, IMC layer could not be detected by SEM. With Ni metallized Cu lead-frame, no IMC layer was observed at the solder/lead-frame as well as Si die/solder interface by SEM. Wetting on Ni metallized Cu lead-frame was found to be lower as compared to that at bare Cu lead-frame. Die shear strength was found to be higher on bare Cu lead-frame (24.2 MPa) as compared to Ni metallized Cu lead-frame (20.5 MPa). Die shear strength of standard Pb-5Sn solder was also measured for comparison and found to be 28.2 MPa.
In vivo comparison of avirulent Vwa- and Pgm- or Pstr phenotypes of yersiniae (1984)

The abilities of Yersinia pestis to undergo restriction in Ca2+-deficient medium with concomitant production of V and W antigens (Vwa+) and to absorb exogenous pigments (Pgm+) are established virulence factors. Mutation of Y. pestis to Pgm- is known to promote resistance to pesticin (Pstr) and reduced lethality by peripheral routes of injection. Vwa+ Pgm- isolates of Y. pestis were shown in this study to retain virulence in mice when injected intravenously. Although Pgm- in appearance, wild-type cells of Yersinia pseudotuberculosis and Yersinia enterocolitica may also be sensitive to pesticin. Pstr mutants of Vwa+ strains of these species were similarly of reduced virulence, especially by peripheral routes of injection. The consequences of mutation to Vwa- and Pgm- or Pstr on growth and persistence in vivo were determined. After intravenous injection, Vwa+ yersiniae of all species exhibited sustained growth in mouse spleen, liver, and lung and accumulated in blood. Septicemia was not observed after similar injection of Vwa- mutants which were unable to maintain comparable rates of net increase in tissues. Mutation to Pgm- or Pstr did not influence proliferation but resulted in enhanced clearance from organs. It is known that reticuloendothelial cells serve as favored sites of replication for all wild-type yersiniae. Our results are consistent with the hypothesis that the Vwa+ phenotype favors growth within macrophages and that the Pgm+ and pesticin-sensitive phenotypes permit long-term, probably extracellular, retention within organs. Virulence in standard animal models (mice, rats, and guinea pigs) was not correlated with resistance to the bactericidal action of serum.
Growth of a yeast of normal alkanes (1964)

Abstract A soil isolate, HD‐5, identified as closely resembling Candida intermedia , was grown on normal alkanes ranging from dodecane (C 12 ) to octadecane (C 18 ). The growth rate of the organism increased with increasing length of the alkane chain. The shortest generation time was 4.5 hr. with octadecane as the carbon source. Cell yields of 82% were common with even numbered alkanes from C 14 through C 18 . The cell yields appeared to decrease with shorter chain (C 12 or below) and odd‐numbered chain alkanes. The cellular nitrogen ranged from 6.9 to 7.5% and the highest lipid content found was 10.3% (with C 17 ).
Oligonucleotide-poly(L-lysine)-heparin complexes: Potent sequence-specific inhibitors of HIV-1 infection (1994)

Poly(L-lysine)-conjugated oligonucleotides complementary to the translation initiation region of the tat protein were tested for their capacity to inhibit HIV-1 replication in de novo infected cells. Sequence-specific antiviral effects were observed with these conjugates at 0.5 microM; their activity was transient, and the viral production was only delayed for a few days. Interestingly, their efficiency was significantly increased by the addition of heparin, a sulfated polyanion that also presents antiviral properties against HIV-1. A single addition, at the time of virus exposure, of the ternary complex formed between oligonucleotide-poly(L-lysine) (75 nM) and heparin (50 micrograms/mL) totally protects cells from HIV-1 infection. Primary interference with virus adsorption is essential for the strong antiviral effect. However, this protection remains strictly sequence specific as demonstrated in experiments performed with different HIV-1 isolates. As comparison, treatments that combine AZT and heparin at the same concentrations did not promote such a complete protection.
Structure and function of human histamine N-methyltransferase: critical enzyme in histamine metabolism in airway (1994)

In mammals, histamine is inactivated principally by two enzymes: histamine N-methyltransferase (HMT; EC 2.1.1.8) and diamine oxidase (DAO; EC 1.4.3.6.). The cDNA clone of human HMT (hHMT) has been isolated from a cDNA library of human kidney and its nucleotide, and deduced amino acid sequences have been determined. One clone, phHMT-1, containing an insert of 1.4 kb, was confirmed to encode HMT by transient expression of HMT activity in COS cells. hHMT consists of 292 amino acid residues [relative molecular weight (M(r)) = 33,279] and shares 82% identity with that of rat HMT. Northern blot analysis with hHMT cDNA probe revealed that 1.6-kb HMT mRNA transcript was expressed in the lung, nasal polyps, and kidney. HMT activity was measured in human trachea and bronchi. In addition, the contractile response of isolated human bronchi to histamine was potentiated in the presence of an HMT inhibitor, SKF 91488, but a DAO inhibitor, aminoguanidine, was without effect. These results suggest that HMT plays an important role in degrading histamine and in regulating the airway response to histamine. Therefore, the level of HMT gene expression in human airway may be one of the critical factors determining the airway responsiveness to histamine. In situ chromosomal hybridization demonstrated that human HMT gene was localized in chromosome 1 p32.
Transvenous cryo-ablation of the slow pathway for the treatment of atrioventricular nodal re-entrant tachycardia: a single-centre initial experience study (2007)

Slow pathway cryo-ablation is associated with an acute success but a higher recurrence rate. A 6-mm-tip cryo-catheter seems to assure during cryo-ablation a large acute and long-term success. AV-block seems non-guaranteed by a negative cryo-mapping, stressing on need of a careful surveillance. Nevertheless, the theoretical advantage of avoiding the risk of permanent AV-block when compared with radiofrequency needs larger series to be demonstrated.
Pulsed-Field Gel Electrophoresis Typing of Oxacillin-Resistant<i>Staphylococcus aureus</i>Isolates from the United States: Establishing a National Database (2003)

Oxacillin-resistant Staphylococcus aureus (ORSA) is a virulent pathogen responsible for both health care-associated and community onset disease. We used SmaI-digested genomic DNA separated by pulsed-field gel electrophoresis (PFGE) to characterize 957 S. aureus isolates and establish a database of PFGE patterns. In addition to PFGE patterns of U.S. strains, the database contains patterns of representative epidemic-type strains from the United Kingdom, Canada, and Australia; previously described ORSA clonal-type isolates; 13 vancomycin-intermediate S. aureus (VISA) isolates, and two high-level vancomycin-resistant, vanA-positive strains (VRSA). Among the isolates from the United States, we identified eight lineages, designated as pulsed-field types (PFTs) USA100 through USA800, seven of which included both ORSA and oxacillin-susceptible S. aureus isolates. With the exception of the PFT pairs USA100 and USA800, and USA300 and USA500, each of the PFTs had a unique multilocus sequence type and spa type motif. The USA100 PFT, previously designated as the New York/Tokyo clone, was the most common PFT in the database, representing 44% of the ORSA isolates. USA100 isolates were typically multiresistant and included all but one of the U.S. VISA strains and both VRSA isolates. Multiresistant ORSA isolates from the USA200, -500, and -600 PFTs have PFGE patterns similar to those of previously described epidemic strains from Europe and Australia. The USA300 and -400 PFTs contained community isolates resistant only to beta-lactam drugs and erythromycin. Noticeably absent from the U.S. database were isolates with the previously described Brazilian and EMRSA15 PFGE patterns. These data suggest that there are a limited number of ORSA genotypes present in the United States.
Compressed Sensing MRI Reconstruction with Cyclic Loss in Generative Adversarial Networks. (2017)

Compressed Sensing MRI (CS-MRI) has provided theoretical foundations upon which the time-consuming MRI acquisition process can be accelerated. However, it primarily relies on iterative numerical solvers which still hinders their adaptation in time-critical applications. In addition, recent advances in deep neural networks have shown their potential in computer vision and image processing, but their adaptation to MRI reconstruction is still in an early stage. In this paper, we propose a novel deep learning-based generative adversarial model, RefineGAN, for fast and accurate CS-MRI reconstruction. The proposed model is a variant of fully-residual convolutional autoencoder and generative adversarial networks (GANs), specifically designed for CS-MRI formulation; it employs deeper generator and discriminator networks with cyclic data consistency loss for faithful interpolation in the given under-sampled k-space data. In addition, our solution leverages a chained network to further enhance the reconstruction quality. RefineGAN is fast and accurate -- the reconstruction process is extremely rapid, as low as tens of milliseconds for reconstruction of a 256x256 image, because it is one-way deployment on a feed-forward network, and the image quality is superior even for extremely low sampling rate (as low as 10%) due to the data-driven nature of the method. We demonstrate that RefineGAN outperforms the state-of-the-art CS-MRI methods by a large margin in terms of both running time and image quality via evaluation using several open-source MRI databases.
Long-term follow-up after radiofrequency catheter ablation for atrial fibrillation (2008)

Catheter ablation does not eliminate paroxysmal AF in up to 56% of patients in the long term, despite the use of two or three ablation procedures in two-thirds of them. However, it confers symptomatic improvement in 67% of treated patients.
Decreasing hydraulic conductivity of Bruch's membrane: Relevance to photoreceptor survival and lipofuscinoses (1995)

Deterioration of visual performance leading to blindness is particularly severe in the early-onset forms of Batten disease. Metabolic support of the neural retina is critically dependent on the choroidal blood supply and on efficient transport pathways through Bruch's membrane and the retinal pigment epithelium (RPE). Conversely, degradation products and, in particular, damaged membranous components of photoreceptor outer segments must be removed in the opposite direction. Incomplete breakdown of damaged "spent" discs leads to the age-related accumulation of lipofuscin-like pigments in the RPE, and these in turn influence the degenerative changes in Bruch's membrane. The generalized and extensive deposition of lipofuscin-like material in Batten disease is therefore likely to exacerbate the degenerative changes in Bruch's membrane, and thereby compromise local fluid dynamics. The hydrodynamic properties of Bruch's membrane were examined in normal donor eyes and showed a precipitous decline of hydraulic conductivity during early life. In fact, the maximal capacity for fluid transport was halved for every 17 years of life. This finding is therefore highly relevant to the development of ensuing pathology in the neuronal ceroid-lipofuscinoses.
Highly reproducible hybridization assay of zeptomole DNA based on adsorption of nanoparticle-bioconjugate (2005)

A nanoparticle-bioconjugate was formed by homogeneous hybridization of one polynucleotide target with two oligonucleotide probes labelled by thiol and a nanoparticle, respectively. Deposition of the nanoparticle-bioconjugate on a gold surface by thiol-gold reaction was monitored in situ by quartz crystal microbalance (QCM) and applied for flow analysis of zeptomole amounts of polynucleotide. The formation in solution and adsorption of thiolated conjugates on gold could be fast, uniform and effective, and has been successfully exploited to construct a highly reproducible and sensitive platform for detection of target sequences. Being more rapid, reproducible, sensitive and amenable to automation than previously reported microgravimetric hybridization assays, this technology has great promise for practical applications in molecular diagnostics.
Oxysterol‐induced osteoblastic differentiation of pluripotent mesenchymal cells is mediated through a PKC‐ and PKA‐dependent pathway (2006)

Abstract Oxysterols form a large family of oxygenated derivatives of cholesterol that are present in circulation, and in human and animal tissues. The discovery of osteoinductive molecules that can induce the lineage‐specific differentiation of cells into osteoblastic cells and therefore enhance bone formation is crucial for better management of bone fractures and osteoporosis. We previously reported that specific oxysterols have potent osteoinductive properties and induce the osteoblastic differentiation of pluripotent mesenchymal cells. In the present report we demonstrate that the induction of osteoblastic differentiation by oxysterols is mediated through a protein kinase C (PKC)‐ and protein kinase A (PKA)‐dependent mechanism(s). Furthermore, oxysterol‐induced‐osteoblastic differentiation is marked by the prolonged DNA‐binding activity of Runx2 in M2‐10B4 bone marrow stromal cells (MSCs) and C3H10T1/2 embryonic fibroblastic cells. This increased activity of Runx2 is almost completely inhibited by PKC inhibitors Bisindolylmaleimide and Rottlerin, and only minimally inhibited by PKA inihibitor H‐89. PKC‐ and PKA‐dependent mechanisms appear to also regulate other markers of osteoblastic differentiation including alkaline phosphatase (ALP) activity and osteocalcin mRNA expression in response to oxysterols. Finally, osteogenic oxysterols induce osteoblastic differentiation with BMP7 and BMP14 in a synergistic manner as demonstrated by the enhanced Runx2 DNA‐binding activity, ALP activity, and osteocalcin mRNA expression. Since Runx2 is an indispensable factor that regulates the differentiation of osteoblastic cells and bone formation in vitro and in vivo, its increased activity in oxysterol‐treated cells further validates the potential role of oxysterols in lineage‐specific differentiation of pluripotent mesenchymal cells and their potential therapeutic use as bone anabolic factors. J. Cell. Biochem. 100: 1131–1145, 2007. © 2006 Wiley‐Liss, Inc.
Bait formulations and longevity of navel orangeworm egg traps tested (2008)

Standardization of pest monitoring practices and materials to maximize sensitivity to pest populations in the field is a foundation of effective integrated pest management (IPM). In response to changes in the availability of commercial bait material for navel orangeworm (NOW) egg traps, we evaluated potential alternative bait materials for use in monitoring this key pest of almonds, pistachios, walnuts and figs. Navel orangeworm egg traps baited with uninfested nutmeats were as effective as almond meal plus 10% crude almond oil, whereas traps baited with freeze-killed, navel orangeworm-infested nutmeats were less effective. The use of nut mummies (culled during winter orchard sanitation) as trap bait may not produce consistent results since the level of navel orangeworm infestation of these nuts is typically unknown. Three seasons of field tests showed that egg traps baited with almond meal plus 3% or 10% crude almond oil received similar numbers of navel orangeworm eggs, and these traps were equally effective for at least 10 weeks.
Aesthetic learning processes – feelings and sensations of skate- and snowboarding (2008)

Skateboarding and snowboarding are practices loaded with symbolic expressions. They are also activities profoundly understood as physical. This paper will investigate the narrated impressions of th ...
NLRP3 Inflammasome and MS/EAE (2013)

Inflammasomes are cytosolic sensors that detect pathogens and danger signals in the innate immune system. The NLRP3 inflammasome is currently the most fully characterized inflammasome and is known to detect a wide array of microbes and endogenous damage-associated molecules. Possible involvement of the NLRP3 inflammasome (or inflammasomes) in the development of multiple sclerosis (MS) was suggested in a number of studies. Recent studies showed that the NLRP3 inflammasome exacerbates experimental autoimmune encephalomyelitis (EAE), an animal model of MS, although EAE can also develop without the NLRP3 inflammasome. In this paper, we discuss the NLRP3 inflammasome in MS and EAE development.
Deoxyhypusine Synthase Generates and Uses Bound NADH in a Transient Hydride Transfer Mechanism (2000)

Deoxyhypusine is a modified lysine residue. It is formed posttranslationally in the precursor of eukaryotic initiation factor 5A (eIF5A) by deoxyhypusine synthase, employing spermidine as a butylamine donor. In the initial step of this reaction, deoxyhypusine synthase catalyzes the production of NADH through dehydrogenation of spermidine. Fluorescence measurements of this reaction revealed a -22-nm blue shift in the emission peak of NADH and a approximately 15-fold increase in peak intensity, characteristics of tightly bound NADH that were not seen by simply mixing NADH and enzyme. The fluorescent properties of the bound NADH can be ascribed to a hydrophobic environment and a rigidly held, open conformation of NADH, features in accord with the known crystal structure of the enzyme. Considerable fluorescence resonance energy transfer from tryptophan 327 in the active site to the dihydronicotinamide ring of NADH was seen. Upon addition of the eIF5A precursor, utilization of the enzyme-bound NADH for reduction of the eIF5A-imine intermediate to deoxyhypusine was reflected by a rapid decrease in the NADH fluorescence, indicating a transient hydride transfer mechanism as an integral part of the reaction. The number of NADH molecules bound approached four/enzyme tetramer; not all of the bound NADH was available for reduction of the eIF5A-imine intermediate.
Invariant Information Local Sub-map Filter (IILSF) for efficient simultaneous localisation and mapping of large environments (2013)

This paper presents an Invariant Information Local Sub-map Filter (IILSF) as a technique for consistent Simultaneous Localisation and Mapping (SLAM) in a large environment. It harnesses the benefits of sub-map technique to improve the consistency and efficiency of Extended Kalman Filter (EKF) based SLAM. The IILSF makes use of invariant information obtained from estimated locations of features in independent sub-maps, instead of incorporating every observation directly into the global map. Then the global map is updated at regular intervals. Applying this technique to the EKF based SLAM algorithm: (a) reduces the computational complexity of maintaining the global map estimates and (b) simplifies transformation complexities and data association ambiguities usually experienced in fusing sub-maps together. Simulation results show that the method was able to accurately fuse local map observations to generate an efficient and consistent global map, in addition to significantly reducing computational cost and data association ambiguities.
An Intrusion Detection and Prevention System based on Automatic Learning of Traffic Anomalies (2016)

The ever changing network traffic reveals new attack types, which represent a security threat that poses a serious risk for enterprise resources. Therefore, the security administrators are in a real need to employ efficient Intrusion Detection and Prevention Systems, IDPS. Such systems might be capable to learn from the network behavior. In this paper, we present an incremental Learnable Model for Anomaly Detection and Prevention of Zero-day attacks, LMAD/PZ. To facilitate the ability of learning from observations that can provide a reliable model for automatic prevention, a comparison has been carried out between supervised and unsupervised learning techniques.
Unfused heterobicycles as amplifiers of phleomycin. III. Thiazolylpyridines and bipyrimidines with strongly basic side chains (1981)

Syntheses are described for 4-(thiazol-4'-yl)pyridines, each 2'-substituted by a dialkylaminoalkyl, dialkylaminoalkylthio or dialkylaminoalkylamino side chain; for 2- and 3-(thiazol-4'-yl)pyridines with a 2'-dimethylaminoethylthio substituent; for 4-(thiazol-2'-yl)pyridines with either a 4'- or a 5'-dialkylaminoalkylcarbamoyl substituent; and for some analogous compounds. The above and similarly substituted 2,4'-, 4,5'- and 5,5'-bipyrimidines have been screened for activity as amplifiers of phleomycin-G against Escherichia coli B by an improved in vitro procedure. The results are tabulated and discussed.