gebhart/patrepeval huggingface dataset:
Maintenance of elevated fetal hemoglobin levels by decitabine during dose interval treatment of sickle cell anemia (2002)

We have previously demonstrated that 5-aza-2'-deoxycytidine (decitabine) augments fetal hemoglobin (HbF) levels in patients with sickle cell anemia (SS) who did not respond to hydroxyurea (HU). The present study was designed to determine the effect of repeated decitabine dosing on HbF levels and hematologic toxicity over a 9-month treatment period. Seven patients (5 HU nonresponders) were entered. One patient had alpha-thalassemia sickle cell anemia. Decitabine was administered by intravenous infusion at a starting dose of 0.3 mg/kg per day, 5 days a week for 2 weeks, followed by a 4-week observation period. If the absolute neutrophil count dropped below 1000, the dose was reduced by 0.05 mg/kg per day in the next cycle. A drug dose was obtained for each patient, and it resulted in an elevated HbF without neutropenia (absolute neutrophil count nadir greater than 1500) or evidence of cumulative toxicity. Average HbF and average maximal HbF levels attained during the last 20 weeks of treatment for the 6 SS patients increased to 13.93% +/- 2.75% and 18.35% +/- 4.46%, respectively, from a pretreatment mean of 3.12% +/- 2.75%. Mean and mean maximal hemoglobin (Hb) levels increased from 7.23 +/- 2.35 g/dL to 8.81 +/- 0.42 g/dL and 9.73 +/- 0.53 g/dL, respectively. Individual maximal F-cell number observed during the trial was 69% +/- 10.12%. The absence of cumulative toxicity may allow shorter intervals between drug treatments, which may lead to higher hemoglobin and HbF levels after several treatment cycles and, therefore, to greater clinical improvement.
Urinary phosphoethanolamine: normal values by age. (1980)

Journal Article Urinary phosphoethanolamine: normal values by age. Get access J R Eastman, J R Eastman Search for other works by this author on: Oxford Academic Google Scholar D Bixler D Bixler Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 26, Issue 12, 1 November 1980, Pages 1757–1758, https://doi.org/10.1093/clinchem/26.12.1757 Published: 01 November 1980
Antibody Expression in Plants (1995)

The development of reliable techniques for transformation of plant cells has led to the possibility of expressing a range of proteins in plants. Of these, expression of antibodies is perhaps the most interesting, because there are so many potential applications. Plants could be used as bio-reactors for large scale antibody production, alternatively, antibodies could be used to modulate plant biochemistry, or influence disease resistance. Antibody processing in plants is very similar to that found in mammalian plasma cells. Signal sequences direct the light and heavy chains to the endoplasmic reticulum for assembly and glycosylation and the assembled antibody is secreted, via the Golgi apparatus. The potential for antibody engineering in plants is high. Modifications to the heavy chain, including the extension of the heavy chain by one domain, can be accomodated without affecting the fidelity of assembly. Antibody fragments can also be produced which may be targeted for secretion, or for intracellular expression by removal of the signal sequence. Secreted antibody accumulates in the extracellular apoplastic fluid, which is a vast, but stable environment, and so levels of recovery can be extremely high.
Diversity-oriented fluorescence library approach for the discovery of sensors and probes (2009)

Fluorescent small molecules have received considerable attention due to their potential for chemosensing and bioimaging. A conventional strategy for probe development is a target-oriented approach based on known molecular recognition mechanisms for individual analytes. However, sophisticated rational design does not always guarantee the applicability of probes in complex biological systems. Therefore the speed and the scope of sensor development has been limited. To overcome these limitations, diversity-oriented fluorescence library approaches have been applied to develop new fluorescent probes in the absence of knowledge about the target recognition mechanism. This review summarizes recent advances in fluorescent probe development facilitated by diversity-oriented library approaches.
Self-assembled <i>N</i>-Alkylimidazolium Perfluorooctanesulfonates (2005)

Abstract Thermostability of a smectic A phase composed of N-alkylimidazolium heptadecafluorooctanesulfonate was improved by the formation of nonpolar layer composed of mixed perfluoroalkyl and aliphatic chains.
Oxygen sensing by ion channels and chemotransduction in single glomus cells. (1996)

We have monitored cytosolic [Ca2+] and dopamine release in intact fura-2-loaded glomus cells with microfluoroimetry and a polarized carbon fiber electrode. Exposure to low PO2 produced a rise of cytosolic [Ca2+] with two distinguishable phases: an initial period (with PO2 values between 150 and approximately 70 mm Hg) during which the increase of [Ca2+] is very small and never exceeds 150-200 nM, and a second phase (with PO2 below approximately 70 mm Hg) characterized by a sharp rise of cytosolic [Ca2+]. Secretion occurs once cytosolic [Ca2+] reaches a threshold value of 180 +/- 43 nM. The results demonstrate a characteristic relationship between PO2 and transmitter secretion at the cellular level that is comparable with the relation described for the input (O2 tension)output (afferent neural discharges) variables in the carotid body. Thus, the properties of single glomus cells can explain the sensory functions of the entire organ. In whole-cell, patch-clamped cells, we have found that in addition to O2-sensitive K+ channels, there are Ca2+ channels whose activity is also regulated by PO2. Ca2+ channel activity is inhibited by hpoxia, although in a strongly voltage-dependent manner. The average hypoxic inhibition of the calcium current in 30% +/- 10% at -20 mV but only 2% +/- 2% at +30 mV. The differential inhibition of K+ and Ca2+ channels by hypoxia helps to explain why the secretory response of the cells is displaced toward PO2 values (below approximately 70 mm Hg) within the range of those normally existing in arterial blood. These data provide a conceptual framework for understanding the cellular mechanisms of O2 chemotransduction in the carotid body.
Treatment with ozone/oxygen‐pneumoperitoneum results in complete remission of rabbit squamous cell carcinomas (2008)

Head and neck squamous cell carcinomas (HNSCC) represent a group of metastasizing tumors with a high mortality rate in man and animals. Since the biomolecule ozone was found to inhibit growth of various carcinoma cells in vitro we here applied the highly aggressive and lethal VX2 carcinoma HNSCC tumor model of the New Zealand White rabbit to test whether ozone exerts antitumorous effects in vivo. Therapeutic insufflation of medical ozone/oxygen (O(3)/O(2)) gas mixture into the peritoneum (O(3)/O(2)-pneumoperitoneum) at an advanced stage of tumor disease led to a survival rate of 7/14 rabbits. Six of the seven surviving rabbits presented full tumor regression and the absence of local or distant lung metastases. Insufflation of pure oxygen (O(2)) resulted in a survival rate of 3/13 animals accompanied by full tumor remission in 2 of the 3 surviving animals. Of the 14 sham-treated animals only 1 had spontaneous tumor remission and survived. No adverse effects or changes in standard blood parameters were observed after repeated intraperitoneal insufflations of the O(3)/O(2) or O(2) gas. Animals with O(3)/O(2)-induced tumor eradication developed tolerance against reimplantation of the VX2 tumor. This could be reversed by immune suppression with a combination of dexamethasone and cyclosporin A suggesting an antitumorous effect of O(3)/O(2)-mediated activation of the body's own immunosurveillance. Although the exact mechanisms of action are still unclear the present data point to O(3)/O(2)-pneumoperitoneum as a promising new strategy in anticancer therapy.
Persistence in the E Language: Issues and implementation (1989)

E is an extension of C++ providing, among other features, database types and persistent objects. In a language offering persistence, there are many important design and implementation issues which must be resolved. This paper discusses some of these issues, comparing the approach taken in the E programming language with other persistent systems
Re-fracturing Horizontal Shale Wells: Case History of a Woodford Shale Pilot Project (2014)

Abstract Multi-stage horizontal well stimulation treatments have been the key completion approach driving the recent US shale revolution. This approach has transformed the industry, making it possible to economically stimulate reservoirs that were previously deemed uneconomic. In attempting to deploy such multi-stage fracture treatments, several tools and processes have been employed during the completion phase. Most popular among these approaches, has been the ‘plug and perf’ technique, which permits multiple treatments to be performed along a horizontal wellbore. This widespread completion approach has allowed these fracturing operations to be rapidly executed, but with the short elapsed time between stages and wells, it has become increasingly challenging for the Completion Engineer to effectively close the evaluation cycle. As many of these shale plays now mature, it is becoming increasingly apparent that the majority of the wells have not been effectively stimulated. Fracture interference and cluster efficiency being among the key concerns with the completion approach, with high efficiency rarely being achieved resulting in only partial coverage. In fulfilling the desire to complete these wells in a timely manner, it is now apparent that there likely remains a significant portion of un-stimulated pay in a typical well post completion. In this paper we will not attempt to address the "Optimized Completion Design", but instead explore techniques that can be used to recomplete some of these wells and recapture a portion of this bypassed pay. The paper will present the planning, the execution and the results of a five well re-fracturing Pilot programme in the Woodford Shale; including a number of reservoir simulation studies. The discussion will also address the well selection process, the re-fracturing approach and the resulting improved production rates.
Determining shape and reflectance of hybrid surfaces by photometric sampling (1989)

A method is presented for determining the shapes of hybrid surfaces without prior knowledge of the relative strengths of the Lambertian and specular components of reflection. The object surface is illuminated using extended light sources and is viewed from a single direction. Surface illumination using extended sources makes it possible to ensure the detection of both Lambertian and specular reflections. Uniformly distributed source directions are used to obtain an image sequence of the object. This method of obtaining photometric measurements is called photometric sampling. An extraction algorithm uses the set of image intensity values measured at each surface point to compute orientation as well as relative strengths of the Lambertian and specular reflection components. The simultaneous recovery of shape and reflectance parameters enables the method to adapt to variations in reflectance properties from one scene point to another. Experiments were conducted on Lambertian surfaces, specular surfaces, and hybrid surfaces. >
Coanda flow over a circular cylinder with injection normal to the surface. (1969)

This paper describes an experimental investigation of a wall jet attached to a circular cylinder with radial injection. The injection, in amounts sufficient to produce separation and redirection of the wall jet, was made through one of several 0.014-in.-wide slits located every 30° on the cylinder surface. Both the attached wall jet and the injection jet were twodimensional incompressible. The working fluid used was air. The ratio of injection momentum at separation to the wall-jet momentum is found to be constant for a given initial wall-jet thickness b. The experiments show that this critical momentum ratio ranges from 0.27 for a small ratio of main slit width to cylinder radius b/a = 0.12, to zero for b/a = 0.518. Such momentum ratios appear to be on the opposite ends of two flow regions which meet when b/a = 0.344 for 30° injection. Here an unstable, switchlike area exists. The low injection requirements, high momentum amplification possible at large b/a could provide very efficient flow control in fluidics and VTOL craft.
The poison Dart frog's batrachotoxin modulates Na <sub>v</sub> 1.8 (2004)

Batrachotoxin is a potent modulator of voltage-gated sodium channels, leading to irreversible depolarisation of nerves and muscles, fibrillation, arrhythmias and eventually cardiac failure. Since its discovery, field researchers also reported numbness after their skin came into contact with this toxin. Intrigued by this phenomenon, we determined the effect of batrachotoxin on the voltage-gated sodium channel Nav1.8, which is considered to be a key player in nociception. As a result, we discovered that batrachotoxin profoundly modulates this channel: the inactivation process is severely altered, the voltage-dependence of activation is shifted towards more hyperpolarised potentials resulting in the opening of Nav1.8 at more negative membrane potentials and the ion selectivity is modified.
Microsoft Excel 2000 Power Programming with VBA (1999)

Hypertension in children is the major risk for cardiovascular disease in adult. Limited data are available for hypertension in children in Southeast Asia. To determine the cause, treatment, and long-term outcome of hypertension in Thai Children, the authors retrospectively studied 62 patients (age <15 years) diagnosed with hypertension at Thammasat University hospital, from December 01, 2004 through November 30, 2009. The mean age was 9.2 +/- 4.2 years; median follow-up 8 months. Fifty five (88.7%) patients had secondary hypertension. Patients presented with stage 2 hypertension (67.7%), and hypertensive emergency (9.7%). Renal parenchymal disease caused 67.3% of secondary hypertension. End organ damage included left ventricular hypertrophy (11.3%) and hypertensive retinopathy (6.4%). Hypertensive emergency was caused mostly by acute poststreptococcal glmoerulnephritis (APSGN, 66.7%). Intravenous diuretic coupled with oral antihypertensive drugs gave uncontrolled blood pressure in APSGN with hypertensive emergency. Seventy percents of patients with essential hypertension had obesity. Majority of the patients had controlled hypertension without medication. Acute poststreptococcal glomerulonephritis remained the common cause of transient hypertension and hypertensive emergency in Thai children. The high incidence of obesity among essential hypertension in children demonstrated in the present study should alert Thai pediatrician to measure blood pressure in every obese child. Treatment of childhood hypertension was associated with good long-term outcome.
Tandem scanning reflected light microscopy: How it works and applications (1986)

The principles of tandem scanning reflected light microscopy and the design of recent instruments are fully described elsewhere and here only briefly. The illuminating light is intercepted by a rotating aperture disc which lies in the intermediate focal plane of a standard LM objective. This device provides an array of separate scanning beams which light up corresponding patches in the plane of focus more intensely than out of focus layers. Reflected light from these patches is imaged on to a matching array of apertures on the opposite side of the same aperture disc and which are scanning in the focal plane of the eyepiece. An arrangement of mirrors converts the central symmetry of the disc into congruency, so that the array of apertures which chop the illuminating beam is identical with the array on the observation side. Thus both illumination and “detection” are scanned in tandem, giving rise to the name Tandem Scanning Microscope (TSM). The apertures are arranged on Archimedean spirals: each opposed pair scans a single line in the image.
A prostaglandin oligomeric derivative inhibits activities of phospholipase and protease: A possible mechanism of membrane protection during ischemia (1989)

A prostaglandin oligomeric derivative was synthesized by alkaline treatment of prostaglandin E1. This compound protected the perfused rat heart from global ischemia. This compound was found to inhibit several lipolytic and proteolytic enzymes in vitro. When phospholipase A2 from Naja naja venom was used as an enzyme and phosphatidylcholine was used as a substrate, 50 per cent inhibition was achieved at 50 microM of the prostaglandin derivative. When trypsin and casein were used as enzyme and substrate, 50 per cent inhibition was obtained at 80 microM. A possible mechanism of beneficial effect of this compound in protecting membranes during ischemia is discussed.
Cystatin C as a potential cerebrospinal fluid marker for the diagnosis of Creutzfeldt‐Jakob disease (2004)

The definite diagnosis of Creutzfeldt-Jakob disease (CJD), the most common form of human prion diseases, relies upon neuropathological data usually obtained at autopsy. In living patients, the diagnosis, based on suggestive clinical features and EEG abnormalities, can be aided by the detection of altered levels of isoforms of the 14-3-3 protein in the cerebrospinal fluid (CSF). However, the validity of this test has been recently challenged and the search for other, more reliable biomarkers for CJD remains highly desirable. The present study describes the identification of a new potential surrogate marker in the CSF of CJD-affected patients. A preliminary study employing surface-enhanced laser desorption/ionization-time of flight (SELDI-TOF) technology highlighted a protein at 13.4 kDa in a small group (n = 8) of CJD-affected patients. Further analysis aimed at identifying this protein using cationic exchange chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed it to be cystatin C. Additional immunoblot assays confirmed that the level of cystatin C was significantly increased (p </= 0.05) in all tested samples (n = 8). We conclude that the analysis of cystatin C levels in CSF could be useful as a pre-mortem indicator of the disease.
Relative fitness models for storage (2006)

Relative fitness is a new black-box approach to modeling storage devices. Whereas conventional black-box models train to predict a device's performance given "device-independent" workload characteristics, relative fitness models learn to predict the changes in performance between specific devices. There are two advantages. First, unlike conventional modeling, relative fitness does not depend entirely on workload characteristics; performance and resource utilization (e.g., cache usage) can also be used to describe a workload. This is beneficial when workload characteristics are difficult to express (e.g., temporal locality). Second, because relative fitness models are constructed for each pair of devices, changes in workload characteristics (e.g., I/O inter-arrival delay) can be modeled. Therefore, unlike a conventional model, a relative fitness model can be used by applications with a closed I/O arrival process. In this article, we present relative fitness as an evolution of the conventional model and share some early results.
Sidewall epitaxial lateral overgrowth of nonpolar a‐plane GaN by metalorganic vapor phase epitaxy (2008)

Abstract A major obstacle to achieving high‐performance devices using nonpolar a‐plane and m‐plane GaN is the existence of high‐density threading dislocations and stacking faults. Low‐defect‐density nonpolar plane GaN films were previously grown by sidewall epitaxial overgrowth using metalorganic vapor phase epitaxy [1, 2]. In this study, we control the growth‐rate ratio of Ga‐polar GaN to N‐polar GaN by adjusting the V/III ratio. It is possible to grow GaN only from the N‐face sidewall of grooves by maintaining a high V/III ratio, which reduces the number of coalescence regions on grooves and decreases the threading‐dislocation density and stacking‐fault density. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Pharmacological profile and anti-ischemic properties of the Ca2+-channel blocker NS-638 (1995)

Included in the sequence of events leading to neuronal death in ischemic tissue following stroke is an excessive and toxic rise in the intracellular Ca2+-concentration, predominantly due to an influx of Ca2+ through nonselective cation-channels as well as Ca2+-channels. In the present study we have characterized the pharmacological profile and anti-ischemic effects of 2-amino-1-(4-chlorobenzyl)-5-trifluoromethylbenzimidazole (NS-638), a small nonpeptide molecule with Ca2 +-channel blocking properties.NS-638 dose dependency inhibited K + -stimulated [45Ca2+1-uptake in chick cortical synaptosomes and 2-amino-3-(3-hydroxy-5-methylisoxazol-4-yl)propionic acid (AMPA)- stimulated [3H]GABA-release from cultured cortical neurons with IC50 values of 2.3 and 4.3 iiM, respectively. K+-stimulated intracellular Ca2+-elevation in cultured cerebellar granule cells was equipotently blocked with an IC50 value of 3.4 /xM. At this concentration no effect on Ca2 +-induced contractions in K+-depolarized guinea pig taenia coli was observed.The effect of NS-638 on neuronal Ca2+-channels was evaluated using whole cell patch clamp techniques. The compound reversibly blocked N- and L-type Ca2+-channels in cultured chick dorsal root ganglion cells in the concentration range of 1-30 fiM.In the mouse middle cerebral artery occlusion (MCAO) model, NS-638 administered i.p. (50 mg kg~1) at 1 h and 6 h post-ischemia, and once a day for the next two days, resulted in a 48% reduction in total infarct volume. The compound did not show protection against ischemic neuronal damage in the gerbil model of bilateral carotid artery occlusion (BCAO). This data suggests, that neuronal Ca2+-channel blockers may have potential in ameliorating the pathological damage after focal ischemia. [Neurol Res 1995; 17: 353-360]
Homogeneous catalyzed reduction of nitro compounds. IV. Selective and sequential hydrogenation of nitroaromatics (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHomogeneous catalyzed reduction of nitro compounds. IV. Selective and sequential hydrogenation of nitroaromaticsJ. F. KniftonCite this: J. Org. Chem. 1976, 41, 7, 1200–1206Publication Date (Print):April 1, 1976Publication History Published online1 May 2002Published inissue 1 April 1976https://pubs.acs.org/doi/10.1021/jo00869a025https://doi.org/10.1021/jo00869a025research-articleACS PublicationsRequest reuse permissionsArticle Views1372Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts