gebhart/patrepeval huggingface dataset:
Collimated attosecond GeV electron bunches from ionization of high-Z material by radially polarized ultra-relativistic laser pulses (2007)

Three dimensional Particle-in-Cell (3D-PIC) simulations of electron acceleration in vacuum with radially polarized ultra-intense laser beams have been performed. It is shown that single-cycle laser pulses efficiently accelerate a single attosecond electron bunch to GeV energies. When multi-cycle laser pulses are used, one has to employ ionization of high-Z materials to inject electrons in the accelerating phase at the laser pulse maximum. In this case, a train of highly collimated attosecond electron bunches with a quasi-monoenergetic spectra is produced. A comparison with electron acceleration by Gaussian laser pulses has been done. It is shown that the radially polarized laser pulses are superior both in the maximum energy gain and in the quality of the produced electron beams.
Relaxed-tolerance optoelectronic device packaging (1991)

The authors present an approach to single-mode fiber coupling which results in relaxed alignment tolerances during the package assembly process. The authors use a ball lens to couple from the optical device either directly to single-mode fiber (SMF), or indirectly to SMF through a fiber lens. The ball lens is aligned and mounted in the first assembly stage. The lens is aligned and fixed either mechanically or semiactively. In the second stage of assembly, the SMF is aligned so as to compensate for any small misalignments of the ball lens. The authors have achieved coupling efficiencies of 33-40 % with this coupling scheme. It has been used for coupling to standard laser diodes and to near-traveling wave optical amplifiers. Fiber-to-fiber gains of 8.5-10 dB have been achieved with packaged devices. The approach provides some versatility with respect to the placement of passive optical components inside the package.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Kynurenine metabolism predicts cognitive function in patients following cardiac bypass and thoracic surgery (2011)

Cardiac surgery involving extra-corporeal circulation can lead to cognitive dysfunction. As such surgery is associated with signs of inflammation and pro-inflammatory mediators activate tryptophan oxidation to neuroactive kynurenines which modulate NMDA receptor function and oxidative stress, we have measured blood concentrations of kynurenines and inflammatory markers in 28 patients undergoing coronary arterial graft surgery and, for comparison, 28 patients undergoing non-bypass thoracic surgery. A battery of cognitive tests was completed before and after the operations. The results show increased levels of tryptophan with decreased levels of kynurenine, anthranilic acid and 3-hydroxyanthranilic acid associated with bypass, and a later increase in kynurenic acid. Levels of neopterin and lipid peroxidation products rose after surgery in non-bypass patients whereas tumour necrosis factor-α and S100B levels increased after bypass. Changes of neopterin levels were greater after non-bypass surgery. Cognitive testing showed that the levels of tryptophan, kynurenine, kynurenic acid and the kynurenine/tryptophan ratio, correlated with aspects of post-surgery cognitive function, and were significant predictors of cognitive performance in tasks sensitive to frontal executive function and memory. Thus, anaesthesia and major surgery are associated with inflammatory changes and alterations in tryptophan oxidative metabolism which predict, and may play a role in, post-surgical cognitive function.
Composite component support for EJB (2004)

Component frameworks and component middleware like Enterprise JavaBeans (EJB) have been established successfully in the last few years. However, composite components are not yet a part of these platforms. They increase reuse of software and can be used to encapsulate run-time adaptation. We present a composite component framework developed as an extension of EJB 2.0. We describe general design objectives for composite components and required rules for the visibility of components. The necessary container support for composite EJBs is implemented based on the EJB application server JBoss. An example application demonstrates the use of the new EJB type and shows how to dynamically reconfigure graphs of composite EJB components after their deployment in order to achieve run-time adaptivity.
Melatonin and vitamin C exacerbate <i>Cannabis sativa</i> -induced testicular damage when administered separately but ameliorate it when combined in rats (2015)

This study further showed the gonadotoxic effect of C. sativa, which could be mediated by oxidative stress. It also showed that melatonin and vitamin C exacerbate C. sativa-induced testicular damage when administered separately but ameliorate it when combined in rats.
An Accelerated Life Test Model Based on Reliability Kinetics (1995)

Inferences from accelerated life tests typically involve extrapolation in stress. For this reason, it is important to use statistical models that have their basis in the physics and chemistry of the important failure mechanism(s). The standard accelerated failure time regression models (based for example on Weibull or lognormal approximations to the failure-time distribution at a given stress and linear scaling of time) are adequate for modeling some simple chemical processes that lead to failure. In this article we present the results from a humidity-accelerated life test of conductive anodic filament failures on printed circuit boards. Standard accelerated life test models are clearly inadequate for these data. Using an approximate chemical kinetic model of the failure process as a basis, we derive an alternative, more general, class of accelerated life test models. We illustrate the use of likelihood-based methods to estimate the model parameters. The new models fit the data better than the traditional accelerated life test models and provide extrapolations that are more consistent with actual field data.
Tumor-associated transplantation antigens in immune rejection of mouse malignant cell hybrids. (1975)

The inability of mouse cell hybrids derived from two malignant parental cells to produce tumors in syngeneic F1 hosts was analyzed. YC hybrids, derived from the fusion of C1.1D and L1210 clls, failed to induce any tumor in adult mice, while 91% of x-irradiated newborn mice developed tumors and died. Some telocentric chromosomes were lacking in hybrid tumors; however, none of the immunologically intact adult mice developed tumors when grafted with tumors grown in x-irradiated newborn mice. This indicates that histocompatibility factors interfered in the failure of YC tumors to grow to adult hosts. Syngeneic F1 mice immunized with Y2C hybrid cells had cytotoxic antibodies against Y2C hybrids and C1.1D and L1210 parental cells. Complete absorption of cytotoxic antibodies directed against hybrid cells by mixtures of both parental cell lines demonstrates the absence of any new antigen on hybrid cells.Hyb rid cells had a higher density of C1.1D-tumor antigenic sites, as compared to C1.1D parental cells.This possibly explains the higher antigenicity and/or the higher sensitivity to immune lysis of hybrid cells.
Differential expression of alpha and beta thyroid hormone receptor genes in rat brain and pituitary. (1989)

Multiple thyroid hormone receptor cDNAs have previously been identified in rat and are classified into alpha and beta subtypes. Alternative splicing of the alpha gene gives rise to the functional receptor, rTR alpha 1, and the non-thyroid hormone-binding isotype, rTR alpha 2. Recent evidence suggests the beta gene encodes two functional receptors, rTR beta 1, and the pituitary-specific receptor, rTR beta 2. By using synthetic DNA probes common to rTR beta transcripts and specific for rTR alpha 1 and rTR alpha 2 mRNAs, we mapped the expression of these transcripts in adult rat brain and pituitary by hybridization histochemistry. We also localized mRNAs encoding the putative nuclear receptor REV-ErbA alpha, a portion of which is derived from the opposite strand of the rTR alpha gene. rTR alpha 1 and rTR alpha 2 transcripts were widely distributed in a similar, if not identical, pattern. Highest levels of rTR alpha 1 and rTR alpha 2 transcripts were found in the olfactory bulb, hippocampus, and granular layer of the cerebellar cortex. REV-ErbA alpha and rTR beta mRNAs were found in more restricted patterns of expression distinct from those of rTR alpha 1 and rTR alpha 2. REV-ErbA alpha mRNA was highest in the neocortex. High levels of rTR beta transcripts in the anterior pituitary and the parvocellular part of the paraventricular hypothalamic nucleus suggest rTR beta gene products may mediate thyroid hormone feedback regulation of thyroid-stimulating hormone and thyrotropin-releasing hormone. Our results identify nuclei and structures in the mammalian central nervous system in which regulation of gene expression by specific thyroid hormone receptor subtypes may occur.
A Fully-Digital EM Pulse Detector (2016)

ElectroMagnetic Pulse Injection (EMPI) has recently been demonstrated to be an efficient fault injection technique with many advantages especially when considering security issues of Systems on Chip (SoC) embedded on ball grid array packages, i.e. when adversaries do not have an easy access to the backside. EMPI must therefore be considered as a real threat against smartcards and SoC from now on. Among the usual countermeasures against fault attacks, one can identify the use of embedded sensors. If one can find voltage glitch or laser shot detectors in the literature, there is only one proposal which puts forward the idea of detecting ElectroMagnetic Pulse (EMP). However, this former sensor requires a fine tuning of some timing characteristics and, as a result, its use appears complex and even impractical within a SoC which are heterogeneous by nature and designed by worldwide teams. Within this context, this paper introduces and experimentally validates a new sensor allowing to detect EMP. Because the sensor is fully digital, it is low cost and above all fully compliant with the standard design flow of SoC.
One-Carbon Metabolism and Alzheimers Disease: Focus on Epigenetics (2010)

Alzheimer's disease (AD) represents the most common form of dementia in the elderly, characterized by progressive loss of memory and cognitive capacity severe enough to interfere with daily functioning and the quality of life. Rare, fully penetrant mutations in three genes (APP, PSEN1 and PSEN2) are responsible for familial forms of the disease. However, more than 90% of AD is sporadic, likely resulting from complex interactions between genetic and environmental factors. Increasing evidence supports a role for epigenetic modifications in AD pathogenesis. Folate metabolism, also known as one-carbon metabolism, is required for the production of S-adenosylmethionine (SAM), which is the major DNA methylating agent. AD individuals are characterized by decreased plasma folate values, as well as increased plasma homocysteine (Hcy) levels, and there is indication of impaired SAM levels in AD brains. Polymorphisms of genes participating in one-carbon metabolism have been associated with AD risk and/or with increased Hcy levels in AD individuals. Studies in rodents suggest that early life exposure to neurotoxicants or dietary restriction of folate and other B vitamins result in epigenetic modifications of AD related genes in the animal brains. Similarly, studies performed on human neuronal cell cultures revealed that folate and other B vitamins deprivation from the media resulted in epigenetic modification of the PSEN1 gene. There is also evidence of epigenetic modifications in the DNA extracted from blood and brains of AD subjects. Here I review one-carbon metabolism in AD, with emphasis on possible epigenetic consequences.
Cloud-based virtual desktop service using lightweight network display protocol (2016)

In Cloud-based VDI, users can access the virtual desktop service anytime and anywhere with the wide variety of terminals. The lightweight virtual desktop display protocol is required to support more users on the limited resources and to provide service to mobile users. In this paper, we design and implement the virtual desktop system using lightweight virtual desktop display protocol which would reduce the server load and network bandwidth consumption for increasing the number of virtual desktops per server and supporting the mobile users. The experimental results show that the implemented protocol reduces the network bandwidth consumption for virtual desktop transfer by up to 81% than previous protocol without compression and optimization.
Cortical Cholinergic Denervation Elicits Vascular Aβ Deposition (2000)

Selective destruction of the cholinergic nucleus basalis magnocellularis (nbm) in the rabbit by the p75 neurotrophin receptor (NTR) immunoglobulin G (IgG) complexed to the toxin saporin leads to the deposition of amyloid-beta (A beta) in and around cerebral blood vessels. In some instances, the perivascular A beta resemble the diffuse deposits observed in Alzheimer's disease (AD). We propose that cortical cholinergic deprivation results, among other perturbations, in the loss of vasodilation mediated by acetylcholine. In addition to a dysfunctional cerebral blood flow, alterations in vascular chemistry affecting endothelial and smooth muscle cells may result in cerebral hypoperfusion and a breached blood-brain barrier (BBB). The selective removal of the rabbit nbm and A beta accumulation may serve as an important nontransgenic, and more physiological, model for the testing of pharmacological and immunological agents designed to control the deposition and the deleterious effects of A beta in AD.
An object-based system for stereoscopic viewpoint synthesis (1997)

This paper describes algorithms that were developed for a real-time stereoscopic videoconferencing systems with viewpoint adaptation. The goal is a real telepresence illusion, which is achieved by synthesis of intermediate views from a stereoscopic camera shot with a rather large baseline. The actual viewpoint will be adapted according to the head position of the viewer, such that the impression of motion parallax is produced. The object-based system first identifies foreground and background regions and applies disparity estimation to the foreground object. A hierarchical block matching algorithm is employed for this purpose which takes into account the position of high-activity feature points and the object/background border positions. Using the disparity estimator's output, it is possible to generate arbitrary intermediate views by projections from the left- and right-view images. For this purpose, we have also developed an object-based interpolation algorithm, taking into account a very simple convex-surface model of a person's face and body. Though the algorithms had to be held rather simple under the constraint of hardware feasibility, we obtain a good quality of the intermediate-view images. Finally, we describe the hardware concept for the disparity estimator, which is the most complicated part of the algorithm.
Symptomatic Intradialytic Hypotension and Mortality: An Opinionated Review (2012)

The unchanging rate of symptomatic hypotension that attends maintenance hemodialysis and its link to sodium/volume overload are explored. Correlations between interdialytic weight gain, ultrafiltration rate, hypertension, and mortality are found to be strong. Suggestions/opinions for correcting this unfortunate clinical reality are offered.
Catalytic and Inhibitory Kinetic Behavior of Horseradish Peroxidase on the Electrode Surface (2012)

Enzymatic biosensors are often used to detect trace levels of some specific substance. An alternative methodology is applied for enzymatic assays, in which the electrocatalytic kinetic behavior of enzymes is monitored by measuring the faradaic current for a variety of substrate and inhibitor concentrations. Here we examine a steady-state and pre-steady-state reduction of H(2)O(2) on the horseradish peroxidase electrode. The results indicate the substrate-concentration dependence of the steady-state current strictly obeys Michaelis-Menten kinetics rules; in other cases there is ambiguity, whereby he inhibitor-concentration dependence of the steady-state current has a discontinuity under moderate concentration conditions. For pre-steady-state phases, both catalysis and inhibition show an abrupt change of the output current. These anomalous phenomena are universal and there might be an underlying biochemical or electrochemical rationale.
Functional Assessment of EnvZ/OmpR Two-Component System in Shewanella oneidensis (2011)

EnvZ and OmpR constitute the bacterial two-component signal transduction system known to mediate osmotic stress response in a number of Gram-negative bacteria. In an effort to understand the mechanism through which Shewanella oneidensis senses and responds to environmental osmolarity changes, structure of the ompR-envZ operon was determined with Northern blotting assay and roles of the EnvZ/OmpR two-component system in response to various stresses were investigated with mutational analysis, quantitative reverse transcriptase PCR (qRT-PCR), and phenotype microarrays. Results from the mutational analysis and qRT-PCR suggested that the EnvZ/OmpR system contributed to osmotic stress response of S. oneidensis and very likely engaged a similar strategy employed by E. coli, which involved reciprocal regulation of two major porin coding genes. Additionally, the ompR-envZ system was also found related to cell motility. We further showed that the ompR-envZ dependent regulation of porin genes and motility resided almost completely on ompR and only partially on envZ, indicating additional mechanisms for OmpR phosphorylation. In contrast to E. coli lacking ompR-envZ, however, growth of S. oneidensis did not show a significant dependence on ompR-envZ even under osmotic stress. Further analysis with phenotype microarrays revealed that the S. oneidensis strains lacking a complete ompR-envZ system displayed hypersensitivities to a number of agents, especially in alkaline environment. Taken together, our results suggest that the function of the ompR-envZ system in S. oneidensis, although still connected with osmoregulation, has diverged considerably from that of E. coli. Additional mechanism must exist to support growth of S. oneidensis under osmotic stress.
Intracellular distribution and mobilization of unesterified cholesterol in adipocytes: triglyceride droplets are surrounded by cholesterol-rich ER-like surface layer structures (2000)

In addition to their central role in triglyceride storage, fat cells are a primary depot of unesterified cholesterol (FC) in the body. In comparison, peripheral cells contain very little FC. This difference in adipocytes versus peripheral tissues is inconsistent with the current theory of cholesterol homeostasis. Attempting to resolve this discrepancy, we examined intracellular storage sites of FC in murine 3T3-F442A adipocytes. Using the cholesterol-binding antibiotic, filipin, in combination with high resolution fluorescence microscopy, intense fluorescent staining characteristically decorated the periphery of triglyceride droplets (TGD) as well as the plasma membrane (PM) of fat cells. Filipin-staining was not visible inside the lipid droplets. Purification of TGD by subcellular fractionation demonstrated that the rise in total FC content of adipocytes upon differentiation was attributable to an increase in TGD-FC, which contributed up to one third of the total cellular FC. The protein component of purified TGD from cultured adipocytes as well as from murine adipocytes obtained from fresh tissues contained the lumenal endoplasmic reticulum (ER) immunoglobulin binding protein (BiP) and the integral ER membrane protein calnexin. Efflux experiments using the extracellular FC acceptors (&bgr;)-cyclodextrin or apolipoprotein A-I demonstrated that TGD-associated FC was releasable from TGD. Whereas FC efflux from adipocytes was unaffected in the presence of brefeldin A or monensin, the secretion of a control protein, lipoprotein lipase, was effectively reduced. In summary, our findings identify the TGD surface layer as primary intracellular storage site for FC within adipocytes. We suggest that the structural role of ER-resident proteins in this adipocyte TGD envelope has been previously neglected. Our findings support the suggestion that an ER-like structure, albeit of modified lipid composition, constitutes the lipid droplets' surface layer. Finally, the efflux process of FC from adipocytes upon extracellular stimulation with (beta)-cyclodextrin provides evidence for an energy-dependent intracellular trafficking route between the TGD-FC pool and the PM-FC sites which is distinct from the secretory pathway of proteins.
Major Conformational Changes Occur during the Transition from an Initiation Complex to an Elongation Complex by T7 RNA Polymerase (2002)

To examine changes that occur during the transition from an initiation complex (IC) to an elongation complex (EC) in T7 RNA polymerase (RNAP), we used nucleic acid-protein cross-linking methods to probe interactions of the RNAP with RNA and DNA in a halted EC. As the RNA is displaced from the RNA-DNA hybrid approximately 9 bp upstream from the active site (at -9) it interacts with a region within the specificity loop (residues 744-750) and is directed toward a positively charged surface that surrounds residues Lys-302 and Lys-303. Surprisingly, the template and non-template strands of the DNA at the upstream edge of the hybrid (near the site where the RNA is displaced) interact with a region in the N-terminal domain of the RNAP (residues 172-191) that is far away from the specificity loop before isomerization (in the IC). To bring these two regions of the RNAP into proximity, major conformational changes must occur during the transition from an IC to an EC. The observed nucleic acid-protein interactions help to explain the behavior of a number of mutant RNAPs that are affected at various stages in the initiation process and in termination.
Three level buck converter with control and soft startup (2009)

The three level buck converter can offer high efficiency and high power density in VR and POL applications. The gains are made possible by adding a flying capacitor that reduces the MOSFET voltage stress by half allowing for the use of low voltage devices, doubles the effective switching frequency, and decreases the inductor size by reducing the volt-second across the inductor. To achieve high efficiency and power density the flying capacitor must be balanced at half of the input voltage and the circuit must be started up without the MOSFETs seeing the full input voltage for protection purposes. This paper provides a new novel control method to balance the flying capacitor with the use of current control and offers a simple startup solution to protect the MOSFETs during start up. Experimental verification shows the efficiency gains and inductance reduction.