gebhart/patrepeval huggingface dataset:
The structure and function of a eukaryotic promoter. (1982)

Transformation, or DNA-mediated gene transfer, permits the stable introduction of new genetic information into a cell and therefore provides an opportunity to examine the expression of exogenous DNA sequences in the transformed host. A series of deletion mutants to analyze the sequences required for accurate and quantitative in vivo transcription of the herpes simplex virus (HSV) thymidine kinase (tk) gene has been constructed. Control of the efficiency of transcription as well as rough specification of the sites of initiation reside within sequences 40-110 nucleotides from the tk structural gene. The sequences responsible for induction of tk mRNA overlap this transcriptional control region. Analysis of the conformation of the integrated tk gene in the chromosome reveals that these DNA sequences are exquisitely sensitive to nuclease attack. The major heat shock gene of Drosophila and the human beta globin gene have also been introduced into murine cells to identify sequences responsible for induction of these finely regulated genes.
Air Movement of Engineering Equipment to Remote Areas (1987)

The transportation of equipment and personnel is one of the major problems to be resolved for the successful development of a remote area. Conventional transportation methods are restricted greatly by the availability of roads or waterways; such facilities are often non-existent or require major upgrading prior to any large scale transport of equipment to the development site. By contrast, large scale air movement only requires the preparation of a small strip of land adjacent to the site, with minimal site support services. This paper will discuss the development of air movement of equipment; Australian capabilities; economic considerations; and future developments of this concept.
Extraction of high frequency power cable characteristics from S-parameter measurements (2004)

A technique is developed for extraction of the wave propagation properties of power cables from S-parameter measurements. The method extracts the complex propagation constant and the characteristic impedance, as well as the LCRG telegrapher's equation parameters. The extraction process is developed after clarifying the effect of the connection between the measurement port and the power cable. It is concluded that treating the connection solely as a characteristic impedance change could lead to considerable errors in the parameter extraction. Furthermore, the method corrects for electrical lengths, which are not accounted for by the standard network analyzer calibration. The extraction is demonstrated for a medium voltage cross linked polyethylene (XLPE) cable over the frequency range 300 kHz to 300 MHz. The results are compared to a time domain short pulse propagation method for cable characterization. Both measurement methods are evaluated against a cable model.
Series II AMTEC cell design and development (1997)

The Series II alkali metal thermoelectric converter (AMTEC) cell, developed in the last year, represents a significant engineering advance in AMTEC technology, and major step toward first market applications. This cell still retains the single artery that characterizes the Series I cell, but has multiple /spl beta/"-alumina solid electrolyte (BASE) tubes connected in electrical series for higher voltage output and improved efficiency. A number of Series II cell design configurations have been tested. The test results have generally confirmed the performance predictions, but have also shown areas where continued development is needed. This paper provides an overview of the Series II cell technology and discusses the implications of results to date.
Dépôts chimiques à partir d’une phase gazeuse (1985)

Parmi les revêtements susceptibles de conférer des propriétés nouvelles aux surfaces qu’ils recouvrent, ceux obtenus par le procédé de dépôts chimiques à partir d’une phase gazeuse (Chemical Vapor Deposition : CVD) connaissent incontestablement un grand développement. Ce procédé consiste à mettre un
Regulation of Cbfa1 Expression by Total Flavonoids of Herba Epimedii (2006)

Core binding factor alpha1 (Cbfa1) is a member of the runt family of transcription factors, which appears to play a pivotal role in regulating the differentiation of osteoblastic precursors and the activity of mature osteoblasts. Total flavonoids of Herba epimedii (HEF) is a recognized bone anabolic agent, but there is lack of reports on the modulation of Cbfa1 expression by HEF. Here we investigated the effect of HEF on Cbfa1 expression in the bone of ovariectomized (OVX) rats. HEF could increase the expression of Cbfa1 mRNA in the bone of ovariectomized rats in a dose-dependent manner. Furthermore, the high dose HEF (160 mg/kg) administered for 12 weeks in vivo stimulated osteocalcin expression. These findings suggest that Cbfa1 is required for mediating the anabolic effects of HEF.
Dynamic cost-effective emergency network provision (2017)

The importance to our society of emergency network communications cannot be underestimated. The loss of communication and network systems in a state of emergency denies victims of disaster and city emergency response teams critical information about the crisis. It is essential to restore communication systems and service in order to ensure continuous and efficient emergency operations. This paper presents a cloud-based cost-effective emergency network management system to provide dynamic network services. We have developed a network application to enable users to access the Internet during an emergency. The application is ready to immediately restore lost connectivity through economical embedded systems and UAVs. We implement our prototypes based on Resin.io, a framework that utilizes Linux containers on IoT(Internet of Things) devices to deploy applications. We evaluate our systems on Raspberry Pi, a popular embedded system. We demonstrate the feasibility of our proposed system, showing that it is an economical infrastructure that it can successfully be used for an emergency network to replace a demolished network infrastructure.
Tryptophan‐2,3‐dioxygenase is regulated by prostaglandin E2 in malignant glioma via a positive signaling loop involving prostaglandin E receptor‐4 (2015)

Malignant gliomas and other types of tumors generate a local immunosuppressive microenvironment, which prohibits an effective anti-tumor immune response and promotes tumor growth. Along with others, we have recently demonstrated that catabolism of the essential amino acid tryptophan via tryptophan-2,3-dioxygenase (TDO) is an important mechanism mediating tumor-associated immunosuppression particularly in gliomas. The pathways regulating TDO in tumors, however, are poorly understood. Here, we show that prostaglandins enhance TDO expression and enzymatic activity in malignant gliomas via activation of prostaglandin E receptor-4 (EP4). Stimulation with prostaglandin E<sub>2</sub> (PGE<sub>2</sub> ) up-regulated TDO-mediated kynurenine release in human glioma cell lines, whereas knockdown of the PGE<sub>2</sub> receptor EP4 inhibited TDO expression and activity. In human malignant glioma tissue expression of the PGE<sub>2</sub> -producing enzyme cyclooxygenase-2 (COX2) and its receptor EP4 were associated with TDO expression both on transcript and protein level. High expression of EP4 correlated with poor survival in malignant glioma patients WHO III-IV. Importantly, treatment of glioma cells with an EP4 inhibitor decreased TDO expression and activity. Moreover, TDO-over-expressing murine gliomas showed increased COX2 and EP4 expression suggesting a positive feedback mechanism in vivo. In summary, targeting EP4 may inhibit - in addition to immunosuppressive COX2 signaling - tryptophan degradation as another important immunosuppressive pathway and thus, could provide a dual clinically relevant immunotherapeutic avenue for the treatment of malignant gliomas. We proposed that in malignant gliomas prostaglandin E<sub>2</sub> (PGE<sub>2</sub> ) produced by cyclooxygenases (COX) up-regulates tryptophan-2,3-dioxygenase (TDO) expression and enzyme activity through binding to its Gs-coupled receptor EP4 and therefore may mediate tumor immune escape in part through aryl hydrocarbon receptor (AHR) activation. Moreover, TDO activity itself seems to induce intratumoral PGE<sub>2</sub> metabolism suggesting an immunosuppressive loop involving COX/EP4/TDO.
Effect of osmotic lysis and resealing on red cell structure and function. (1990)

We have recently modified the dialysis tubing osmotic lysis and resealing method to examine the role of intracellular red blood cell (RBC) antioxidants. However, the potential effect of resealing on the RBC was not fully investigated. This study examined a number of cellular characteristics to determine the effects of physical lysis and resealing on the RBC. Following resealing, RBC exhibited normal morphology and at most only slight alterations in mean cell volume and mean cell hemoglobin concentration. RBC density distribution was significantly affected by resealing with increased populations of both light and dense cells, though the mean cell density was similar to that of control cells. Endogenous enzyme activities and adenosine triphosphate (ATP) concentration were unaffected by the resealing procedure. While reduced glutathione (GSH) concentration was decreased by 15%, RBC oxidant sensitivity was found to be unaltered. Cellular deformability of the resealed RBC was 80% to 90% that of the control cells. Membrane phospholipid and fatty acyl composition of the resealed RBC were unaffected when compared with matched control samples. Membrane transport, permeability, and Ca2(+)-mediated cellular vesiculation were minimally altered by resealing. Finally, entrapment of fluorescent compounds demonstrated that greater than 95% of the resealed RBC had incorporated exogenous agents. In summary, the osmotic lysis and resealing method described resulted in only minor changes in cellular characteristics while allowing for the efficient loading of compounds to which the RBC membrane is normally impermeable. Consequently, this method provides great potential for the selective modification of erythrocyte constituents in order to further define their roles within the RBC.
Chemistry of the vanadium-carbon .sigma. bond. 3. Reactivity of a homoleptic tris(.eta.2-iminoacyl) complex of vanadium(III) (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of the vanadium-carbon .sigma. bond. 3. Reactivity of a homoleptic tris(.eta.2-iminoacyl) complex of vanadium(III)Javier Ruiz, Marilin Vivanco, Carlo Floriani, Angiola Chiesi-Villa, and Corrado RizzoliCite this: Organometallics 1993, 12, 5, 1811–1822Publication Date (Print):May 1, 1993Publication History Published online1 May 2002Published inissue 1 May 1993https://pubs.acs.org/doi/10.1021/om00029a043https://doi.org/10.1021/om00029a043research-articleACS PublicationsRequest reuse permissionsArticle Views186Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A New Wafer Level Packaging Approach: Encapsulation, Metallization and Laser Structuring for Advanced System in Package Manufacturing (2005)

One of the general trends in microelectronics packaging is the constant miniaturization of devices. This has led to the development of maximum miniaturization of components on Si level, i.e., CSPs and Flip Chips. To further integrate more functionality into devices, and to further increase the degree of miniaturization, packaging development focus is switching from single chip packaging to the realization of systems in package, SiPs. Two main approaches do exist to realize this goal: one is to integrate all components into one dedicated package, yielding maximum miniaturization for a special application, but little flexibility as far as system design is concerned. The other is to create modular stackable components that can be assembled into a functional system. This integrates both flexibility in system design by exchangeable components and increased reliability potential, as single components can be tested separately. This last approach was considered a promising choice for the generation of SiPs. Within this paper a packaging process is introduced that allows the wafer level manufacturing of stackable, encapsulated devices. Using a transfer molded epoxy demonstrator, a proof-of-concept is performed showing the feasibility of the stackable package approach. This is achieved by combining wafer level encapsulation and molded interconnect device technology. An electroless process for metallization and laser techniques for structuring the metallization layer have been applied to generate structures for reliable interconnects capable for the use of lead-free solders. Summarized, this paper presents the process development and feasibility analysis of wafer level packaging technologies for modular SiP solutions based on a duromer MID approach.
Cytokine production by synovial T cells in rheumatoid arthritis (1999)

These data clearly demonstrate production of cytokines by T cells in RA synovial tissue, indicating that activated T cells play a role in the pathophysiological events of RA.
Laser endotaxy in silicon carbide and PIN diode fabrication (2008)

A laser solid phase diffusion technique has been utilized to fabricate endolayers in n-type 6H-SiC substrates by carbon incorporation. X-ray energy dispersive spectroscopic analysis shows that the thickness of the endolayer is about 100 nm. High resolution transmission electron microscopic images indicate that the laser endotaxy process maintains the crystalline integrity of the substrate without any amorphization. The resistivity of the endolayer formed in a 1.55 Ω cm silicon carbide wafer segment was found to be 1.1×105 Ω cm, which is sufficient for device fabrication and isolation. Annealing at 1000 °C for 10 min to remove hydrogen resulted in a resistivity of 9.4×104 Ω cm. The endolayer and parent silicon carbide epilayer were doped with aluminum using a laser doping technique to create p-regions on the top surfaces of the substrates in order to fabricate p-type-intrinsic type-n-type (PIN) diodes. The current-voltage characteristics of these diodes were compared with other PIN diodes fabricated using epilayers and other doping techniques.
Cyber Threats Facing Autonomous and Connected Vehicles: Future Challenges (2017)

Vehicles are currently being developed and sold with increasing levels of connectivity and automation. As with all networked computing devices, increased connectivity often results in a heightened risk of a cyber security attack. Furthermore, increased automation exacerbates any risk by increasing the opportunities for the adversary to implement a successful attack. In this paper, a large volume of publicly accessible literature is reviewed and compartmentalized based on the vulnerabilities identified and mitigation techniques developed. This review highlighted that the majority of studies are reactive and vulnerabilities are often discovered by friendly adversaries (white-hat hackers). Many gaps in the knowledge base were identified. Priority should be given to address these knowledge gaps to minimize future cyber security risks in the connected and autonomous vehicle sector.
Fast-light for astrophysics: super-sensitive gyroscopes and gravitational wave detectors (2007)

We present a theoretical analysis and experimental study of the behaviour of optical cavities filled with slow- and fast-light materials, and show that the fast-light material-filled cavities, which can function as ‘white light cavities’, have properties useful for astrophysical applications such as enhancing the sensitivity-bandwidth product of gravitational wave detection and terrestrial measurement of Lense–Thirring rotation via precision gyroscopy.
Cutaneous sarcoidosis: an immunofluorescence study (1983)

Biopsy specimens from granulomatous skin lesions of 14 patients with active sarcoidosis were examined by immunofluorescence microscopy. In seven lesions, deposits of IgM, IgA or complement C3 were demonstrated in the dermal vessel walls and/or at the dermal-epidermal junction. Similar deposits were found in three of twelve biopsy specimens from clinically normal skin from a buttock of the same patients. The results support the hypothesis that deposition of circulating immune complexes in the vessel walls may be of importance for the development of granulomatous lesions in sarcoidosis.
Cytokine-mediated communication between dendritic epidermal T cells and Langerhans cells. In vitro studies using cell lines (1996)

Murine epidermis contains two leukocyte populations: Langerhans cells (LC), which are APC of dendritic cell (DC) lineage, and dendritic epidermal T cells (DETC), which are members of the tissue-type gamma delta T cell family. Despite close physical approximation in vivo, the extent to which LC and DETC affect each other's function has remained unknown. We addressed this question using the long term DC line XS52 and the gamma delta T cell line 7-17, both of which were established from mouse epidermis, and both of which retain important features of the resident populations from which they were derived. XS52 DC proliferated maximally when cocultured with gamma-irradiated 7-17 DETC. They also proliferated in response to culture supernatants collected from anti-CD3- or Con A-activated 7-17 DETC, but not from nonstimulated DETC. In both systems, DETC-induced XS52 DC growth was inhibited partially (up to 70%) by Abs against granulocyte/macrophage CSF (GM-CSF) or CD115 (CSF-1 receptor) and nearly completely (up to 90%) by both together. Among 28 tested cytokines, only GM-CSF, CSF-1, IL-4, and IL-13 promoted XS52 DC growth significantly. Anti-IL-4 failed to inhibit DETC-induced XS52 cell growth, and IL-4 was not detectable in DETC supernatants. Thus, we conclude that GM-CSF and CSF-1 (and perhaps IL-13) account for the DC growth-promoting activity secreted by DETC. These results suggest that during coculture, XS52 DC activate 7-17 DETC to secrete both GM-CSF and CSF-1. In fact, when cultured with XS52 DC, 7-17 DETC also elevated their expression of the gamma c receptor and acquired proliferative responsiveness to their own growth factor IL-15. We propose that LC and DETC in situ may interact with each other in a similar manner, thereby regulating their residence and function.
High prevalence of TT virus infection in Brazilian blood donors (1999)

A recent report has described the molecular cloning and characterization of a novel, single-stranded DNA virus, named TT virus (TTV), which was present in the sera of Japanese patients with posttransfusion hepatitis of unknown etiology [Okamoto et al. (1998) Hepatology Research 10:1-16]. Using a nested polymerase chain reaction assay, sera from Brazilian patients with acute non A-C hepatitis and blood donors were examined for the presence of TTV DNA sequences. Thirty-seven of 52 (71%) patients with acute non A-C hepatitis and 45 of 72 (62%) blood donors were found to have TTV sequences in their sera. Such a high proportion in blood donors indicated that TTV infection is common in the general Brazilian population. Partial nucleotide sequences (326 bases in open reading frame 1) from seven isolates were determined. By phylogenetic analysis, four TTV strains were classified into the genomic subgroup G1a described previously. The three others belonged to subgroup G1b. Sequence homologies between strains belonging to a same subgroup were 92.9-99.1%, whereas homologies of 85.9-90.2% were calculated between isolates from different subgroups.
Adhesion molecules and atherogenesis (2001)

Atherosclerosis is an inflammatory disease of the vessel wall characterized by monocyte infiltration in response to pro-atherogenic factors such as oxidized lipids. Recently, the role of specific adhesion molecules in this process has been explored. The endothelium overlying atherosclerotic lesions expresses P-selectin and the shoulder regions express vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1), which is also expressed on endothelium in regions not prone to plaque development. Serum levels of soluble P-selectin, ICAM-1 and VCAM-1 are elevated in patients with angina pectoris or peripheral atherosclerotic disease. Reconstituted in vitro systems using monocytes on cytokine-activated endothelial cells under shear flow suggested the involvement of P-selectin, L-selectin, VCAM-1, its ligand, VLA-4 integrin and CD18 integrins. Studies of monocyte adhesion in isolated perfused carotid arteries harvested from atherosclerotic (apoE-/-) mice show a predominant involvement of P-selectin and its ligand P-selectin glycoprotein-1 (PSGL-1) in rolling and of VLA-4 and VCAM-1 in firm adhesion. Consistent with these findings, apoE-/- mice that are also deficient for P-selectin show significantly reduced atherosclerotic lesion sizes and are almost completely protected from neointimal growth after vascular injury. Milder effects are also seen in the low-density lipoprotein (LDL) receptor deficient (LDLR-/-) mouse. In a high cholesterol/cholate model, a role of ICAM-1 and CD18 integrins was also shown, but this awaits confirmation in more physiologic models. Transient blockade of the VLA-4/VCAM-1 adhesion pathway by antibodies or peptides in apoE-/- or LDLR-/- mice reduced monocyte and lipid accumulation in lesions. These data suggest that P-selectin, PSGL-1, VLA-4 and VCAM-1 are the most important adhesion molecules involved in monocyte recruitment to atherosclerotic lesions.
Autosis and autophagic cell death: the dark side of autophagy (2014)

It is controversial whether cells truly die via autophagy or whether - in dying cells - autophagy is merely an innocent bystander or a well-intentioned 'Good Samaritan' trying to prevent inevitable cellular demise. However, there is increasing evidence that the genetic machinery of autophagy may be essential for cell death in certain settings. We recently identified a novel form of autophagy gene-dependent cell death, termed autosis, which is mediated by the Na(+),K(+)-ATPase pump and has unique morphological features. High levels of cellular autophagy, as occurs with treatment with autophagy-inducing peptides, starvation, or in vivo during certain types of ischemia, can trigger autosis. These findings provide insights into the mechanisms and strategies for prevention of cell death during extreme stress conditions.