gebhart/patrepeval huggingface dataset:
Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation. (1997)

Transcription by RNA polymerase III involves recruitment of the polymerase by template-bound accessory factors, followed by initiation, elongation, and termination steps. An immunopurification approach has been used to demonstrate that human RNA Pol III is composed of 16 subunits, some of which are apparently modified in HeLa cells. Partial denaturing conditions and sucrose gradient sedimentation at high salt result in the dissociation of a subcomplex that includes hRPC32, hRPC39, and hRPC62. Cognate cDNAs were isolated and shown to encode three subunits that are specific to RNA Pol III and homologous to three yeast subunits. The human RNA Pol III core lacking the subcomplex functions in transcription elongation and termination following nonspecific initiation on a tailed template, but fails to show promoter-dependent transcription initiation in conjunction with accessory factors. The capability for specific transcription initiation can be restored either by the natural subcomplex or by a stable subcomplex composed of recombinant hRPC32, hRPC39, and hRPC62 polypeptides. One component (hRPC39) of this subcomplex interacts physically with both hTBP and hTFIIIB90, two subunits of human RNA Pol III transcription initiation factor IIIB. These data strongly suggest that the hRPC32-hRPC39-hRPC62 subcomplex directs RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and hRPC39.
Long‐term follow‐up of early dopa treatment in Parkinson's disease (1986)

A group of 19 patients with Parkinson's disease, who began dopa therapy 1 to 3 years after onset of symptoms, were followed for 12 years. They were evaluated every 3 to 4 months on the UCLA disability scale and the Hoehn and Yahr functional classification scale. This present group was compared to two other historical groups reported on earlier [8], one that began treatment with dopa 4 to 6 years after onset of symptoms and the other that began dopa treatment 7 to 9 years after onset of symptoms. Results on both scales showed that the mean disability scores of the three groups did not differ significantly when duration of disease was matched, even though duration of dopa therapy among the groups varied from 1 to 12 years. The highly significant differences between the groups prior to dopa treatment continued throughout the study. These longitudinal data support the conclusion that the worsening over time is the result of progression of Parkinson's disease and not a result of the duration of dopa treatment. Early treatment improves the beginning years of the disease and has no adverse effect on later years. Twelve years after beginning dopa therapy, 32% of the present group had died at a mean age of 76.0, in contrast to 50% (mean age, 74.7) and 57% (mean age, 71.5) in the groups who began dopa increasingly later in the course of their disease. Incidence of dementia increased with disease duration regardless of treatment duration.(ABSTRACT TRUNCATED AT 250 WORDS)
Management of Large Infected Tibial Defects with Antibiotic Beads Local Therapy and Staged Fibular Osteoseptocutaneous Free Transfer (1997)

Fifteen patients with tibial fractures complicated by large infected tibial defects were treated with a two-stage protocol. In the first stage, antibiotic-impregnated polymethylmethacrylate bead chains were used to obliterate the debrided osseous defect, and a meshed porcine skin was used for temporary wound coverage. In the second stage, the bead chains were removed, and the defects were reconstructed with a microvascularized fibular osteoseptocutaneous free transfer. The time between the first and second stages of treatment was 2 to 6 weeks. The bone defects ranged from 6 to 16 cm, and the skin defect areas ranged from 10 to 82 cm2. Wound healing and bony union were achieved in all 15 cases. An additional muscle flap or skin graft was required for only three patients with a large skin defect. Minor pin tract infections were seen in two patients. Stress fractures in three cases were successfully managed with bracing, external skeletal fixation or plating, and cancellous bone grafting. All of the most recent roentgenograms showed good consolidation and hypertrophy of grafted fibular bones. No recurrence of osteomyelitis was observed during an average follow-up period of 50 months (range, 36-86 months). We therefore conclude that this treatment protocol provides rapid recovery from osteomyelitis. The fibular osteoseptocutaneous graft is a useful method for the reconstruction of a large tibial defect, and it also offers the unique advantage of simultaneously reconstructing a moderate skin defect.
Fortification of the Diet as a Strategy for Preventing Iron Deficiency (1989)

Progress in the fortification of food with iron is reviewed in the light of the different circumstances prevailing in the developed and developing world. Although fortification programmes are well established in developed countries many were not based on sound principles and there is no good evidence that they have proved effective. The important steps in establishing a fortification programme include accurate establishment of the need for iron fortification in a particular group or population, the choice of an appropriate combination of food vehicle and iron compound which is both stable and acceptable, and confirmation that the iron in the chosen blend is bioavailable and in different dietary settings. Only after the successful completion of controlled field trials using the chosen combination is the establishment of a regional or national fortification programme worthwhile. These points are illustrated by the success of field trials in the developing world, at least one of which has led to the implementation of a national programme directed to infants and children. Finally, a case study is described in which these principles were followed in the planning and execution of a successful field trial in an iron deficient Indian population in South Africa. The vehicle was curry powder and the iron compound Fe(111)EDTA.
Association of Dietary Patterns With Risk of Colorectal Cancer Subtypes Classified by <i>Fusobacterium nucleatum</i> in Tumor Tissue (2017)

Prudent diets rich in whole grains and dietary fiber are associated with a lower risk for F nucleatum-positive colorectal cancer but not F nucleatum-negative cancer, supporting a potential role for intestinal microbiota in mediating the association between diet and colorectal neoplasms.
Understanding the mechanisms of efficacy of fecal microbiota transplant in treating recurrent <i>Clostridioides difficile</i> infection and beyond: the contribution of gut microbial-derived metabolites (2020)

Fecal microbiota transplant (FMT) is a highly-effective therapy for recurrent <i>Clostridioides difficile</i> infection (rCDI), and shows promise for certain non-CDI indications. However, at present, its mechanisms of efficacy have remained poorly understood. Recent studies by our laboratory have noted the particular key importance of restoration of gut microbe-metabolite interactions in the ability of FMT to treat rCDI, including the impact of FMT upon short chain fatty acid (SCFAs) and bile acid metabolism. This includes a significant impact of these metabolites upon the life cycle of <i>C. difficile</i> directly, along with potential postulated additional benefits, including effects upon host immune response. In this <i>Addendum</i>, we first present an overview of these recent advancements in this field, and then describe additional novel data from our laboratory on the impact of FMT for rCDI upon several gut microbial-derived metabolites which had not previously been implicated as being of relevance.
Cyclodextrin catalysis in the intramolecular Diels-Alder reaction with the furan diene (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCyclodextrin catalysis in the intramolecular Diels-Alder reaction with the furan dieneDaniel D. Sternbach and Debby M. RossanaCite this: J. Am. Chem. Soc. 1982, 104, 21, 5853–5854Publication Date (Print):October 1, 1982Publication History Published online1 May 2002Published inissue 1 October 1982https://doi.org/10.1021/ja00385a081RIGHTS & PERMISSIONSArticle Views508Altmetric-Citations69LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (296 KB) Get e-Alerts Get e-Alerts
Magnetodielectric Thin Film Heterostructure With High Permeability and Permittivity (2009)

This paper discusses combined high-permeability and high-permittivity magnetodielectric thin films for microwaves. The results are based on outstanding material properties: epsiv = 18, mu <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">dc</sub> = 180, f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">r</sub> = 4.8 GHz, and Deltaf= 480 MHz. This material combines exchange coupled high-magnetization ferromagnetic thin films and SrTiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> materials as dielectric lamination. The benefit of such magnetodielectric material is expected for size reduction of 1/2 wave resonators. To this end, this material has been integrated with different coplanar waveguides (CPW) structures. Here, we discuss the effect of increasing the overlap from the central part of ribbon to the lateral grounds. The potential of miniaturization based on the enhancement of the constant of propagation is evaluated for different configurations up to 40%. We also show that the conductivity of the ferromagnetic layers may play a key role in the propagation when the material overlaps the gap of the CPW lowering the expected benefit of the permittivity.
Characterization of the Ground-to-Air Ranging Performance of the 960-1215 MHz ARNS Band Using OFDM Measurements in the 902-928 MHz ISM Band (2016)

In this paper we discuss the feasibility of prototyping radionavigation hardware, designed to operate in the ARNS band, by flight testing it in the 902-928 MHz ISM band. A central challenge in this effort was the shared nature of the ISM band, which causes high in-band interference levels and, consequently, a reduction in the signal-to-noise ratio. We propose mitigating strategies that enable operation in the ISM band, as well as a methodology for assessing the equivalence of results with ARNS band measurements.
Managing Data Quality in Dynamic Decision Environments (2003)

Large data volumes, widely distributed data sources, and multiple stakeholders characterize typical e-business settings. Mobile and wireless technologies have further increased data volumes, further distributed the data sources, while permitting access to data anywhere, anytime. Such environments empower and necessitate decision-makers to act/react quicker to all decision-tasks including mission-critical ones. Decision-support in such environments demands efficient data quality management. This paper presents a framework for managing data quality in such environments using the information product approach. It includes a modeling technique to explicitly represent the manufacture of an information product, quality dimensions and methods to compute data quality of the product at any stage in the manufacture, and a set of capabilities to comprehensively manage data quality and implement total data quality management. The paper also posits the notion of a virtual business environment to support dynamic decision-making and describes the role of the data quality framework in this environment.
Electric-Field-Dependent Charge-Carrier Velocity in Semiconducting Carbon Nanotubes (2005)

Charge transport in semiconducting single-walled nanotubes (SWNTs) with Schottky-barrier contacts has been studied at high bias. We observe nearly symmetric ambipolar transport with electron and hole currents significantly exceeding 25 microA, the reported current limit in metallic SWNTs due to optical phonon emission. Four simple models for the field-dependent velocity (ballistic, current saturation, velocity saturation, and constant mobility) are studied in the unipolar regime; the high-bias behavior is best explained by a velocity-saturation model with a saturation velocity of 2 x 10(7) cm/s.
DESIGN OPTIMIZATION OF THE HOLOS-QUAD MICRO-REACTOR CONCEPT (2021)

The Holos-Quad micro-reactor concept is proposed by HolosGen LLC to generate 22 MWt with a lifetime of approximately 8 effective full power years (EFPYs) for civilian applications. The design is based on a very innovative high-temperature gas-cooled reactor concept using four Subcritical Power Modules (SPMs) that fit into one commercial 40-foot transport ISO container. A rigorous design approach was employed in order to ensure that all input parameters were fully investigated and the best solutions possible were considered. The first step of this approach consisted of properly defining the Holos-Quad design problem by identifying the design objectives, the operational constraints, and the input parameters. In the second step, a sensitivity analysis was performed to enable a preliminary investigation of the input parameters to identify the correlations among input and output variables. Finally, the design optimization was performed in the third step, employing a genetic algorithm to effectively explore the highly constrained input parameters and find global optimal solutions. The multi-objective optimization identified various core solutions that would be optimum solutions for different types of applications. For applications where economics matters less and the ease of transportation matters more, a core weight of ~15 tons could achieve a lifetime of ~3.5 EFPYs. For applications where economics matters more and the ease of transportation matters less, a lifetime of 8.3 EFPYs could be achieved with a total core weight of ~26.7 tons.
Rapid trace detection of triacetone triperoxide (TATP) by complexation reactions during desorption electrospray ionization (2006)

Desorption electrospray ionization (DESI) mass spectrometry is used for rapid, specific and sensitive detection of trace amounts of the notorious explosive TATP present on ambient surfaces by alkali metal complexation in a simple spray technique.
A fault detection and diagnosis approach for multi-tier application in cloud computing (2020)

Ensuring the availability of cloud computing services always concerns both service providers and end users. Therefore, the system always needs precautions for unexpected cases. Accordingly, cloud computing services must be capable of identifying faults and behaving appropriately when it is abnormal to ensure the smoothness as well as the service quality. In this study, we propose a fault detection method for multi-tier web application in cloud computing deployment environment based on the Fuzzy One-class support vector machine and Exponentially Weighted Moving Average method. And then, the suspicious metrics are located by using feature selection method which based on Random Forest algorithm. To evaluate our approach, a multi-tier application is deployed by a transnational web e-Commerce benchmark by using TPC-W (TPC Benchmark™ W, simulates the activities of a business oriented transaction web server in a controlled internet commerce environment) in private cloud and then it is injected typical faults. The effectiveness of the fault detection and diagnosis are demonstrated in experiment results.
Val216 decides the substrate specificity of α‐glucosidase in <i>Saccharomyces cerevisiae</i> (2004)

Differences in the substrate specificity of alpha-glucosidases should be due to the differences in the substrate binding and the catalytic domains of the enzymes. To elucidate such differences of enzymes hydrolyzing alpha-1,4- and alpha-1,6-glucosidic linkages, two alpha-glucosidases, maltase and isomaltase, from Saccharomyces cerevisiae were cloned and analyzed. The cloned yeast isomaltase and maltase consisted of 589 and 584 amino acid residues, respectively. There was 72.1% sequence identity with 165 amino acid alterations between the two alpha-glucosidases. These two alpha-glucosidase genes were subcloned into the pKP1500 expression vector and expressed in Escherichia coli. The purified alpha-glucosidases showed the same substrate specificities as those of their parent native glucosidases. Chimeric enzymes constructed from isomaltase by exchanging with maltase fragments were characterized by their substrate specificities. When the consensus region II, which is one of the four regions conserved in family 13 (alpha-amylase family), is replaced with the maltase type, the chimeric enzymes alter to hydrolyze maltose. Three amino acid residues in consensus region II were different in the two alpha-glucosidases. Thus, we modified Val216, Gly217, and Ser218 of isomaltase to the maltase-type amino acids by site-directed mutagenesis. The Val216 mutant was altered to hydrolyze both maltose and isomaltose but neither the Gly217 nor the Ser218 mutant changed their substrate specificity, indicating that Val216 is an important residue discriminating the alpha-1,4- and 1,6-glucosidic linkages of substrates.
Relevancy Ranking of Web Services (2007)

In order for Web services to truly become a standard approach for just-in-time application integration, we need to enhance the discovery mechanism by assisting clients to select relevant Web services of interest. In recent years, there have been several standards that regulate how services can be published, discovered, or used (i.e. UDDI, WSDL, SOAP). Many of these standards have the potential of enhancing the discovery process, however, there are major technical challenges associated with these standards. One of these challenges is the client’s ability to control and mange the discovery process for finding Web services of interest. Clients should be able to find relevant services much more efficiently. To address this issue, we propose a Web Service Ranking (WSR) algorithm for measuring the relevancy of Web services to particular clients’ requirements. This paper presents experimental validation, results and analysis of the presented ideas.
Automated Pulmonary Nodule Classification in Computed Tomography Images Using a Deep Convolutional Neural Network Trained by Generative Adversarial Networks (2019)

Lung cancer is a leading cause of death worldwide. Although computed tomography (CT) examinations are frequently used for lung cancer diagnosis, it can be difficult to distinguish between benign and malignant pulmonary nodules on the basis of CT images alone. Therefore, a bronchoscopic biopsy may be conducted if malignancy is suspected following CT examinations. However, biopsies are highly invasive, and patients with benign nodules may undergo many unnecessary biopsies. To prevent this, an imaging diagnosis with high classification accuracy is essential. In this study, we investigate the automated classification of pulmonary nodules in CT images using a deep convolutional neural network (DCNN). We use generative adversarial networks (GANs) to generate additional images when only small amounts of data are available, which is a common problem in medical research, and evaluate whether the classification accuracy is improved by generating a large amount of new pulmonary nodule images using the GAN. Using the proposed method, CT images of 60 cases with confirmed pathological diagnosis by biopsy are analyzed. The benign nodules assessed in this study are difficult for radiologists to differentiate because they cannot be rejected as being malignant. A volume of interest centered on the pulmonary nodule is extracted from the CT images, and further images are created using axial sections and augmented data. The DCNN is trained using nodule images generated by the GAN and then fine-tuned using the actual nodule images to allow the DCNN to distinguish between benign and malignant nodules. This pretraining and fine-tuning process makes it possible to distinguish 66.7% of benign nodules and 93.9% of malignant nodules. These results indicate that the proposed method improves the classification accuracy by approximately 20% in comparison with training using only the original images.
Development of a Low Flow Resistance Intravenous Oxygenator (1997)

A potentially attractive support device for patients with acute respiratory failure is an intravenous membrane oxygenator. One problem, however, is that the membrane surface area required for sufficient gas exchange can unduly increase vena cavai pressure drop and impede venous return. The purpose of this study was to design and develop an intravenous oxygenator that would offer minimal venous flow resistance in situ. The device uses a constrained fiber bundle of smaller cross sectional size than the vena cava so as to effect an intentional shunt flow of venous blood around the fiber bundle and reduce the venous pressure drop caused by the device. A pulsating balloon within the fiber bundle redirects part of this shunt flow into reciprocating flow in and out of the fiber bundle. This offers dual advantages: 1) Blood flow through the fiber bundle is mainly perpendicular to the fibers; and 2) the requisite energy for driving flow comes largely
Light induced DNA–protein conjugation (2013)

DNA was modified with a photo-reactive caged diene allowing for the modification of dienophile containing proteins under mild irradiation conditions to afford fully functional DNA-protein conjugates.
Espacios comunes del diseño y la innovación (2006)

En el presente articulo Marta Carrio identifica los espacios de confluencia entre el diseno y la innovacion en el marco del desarrollo de productos a partir del analisis de los estudios y los trabajos disponibles en este ambito de actividad, e introduce las potencialidades que supone el hecho de incorporar el diseno al proceso de innovacion.
The group data access control method in content centric network (2015)

Usage of the Internet for content delivery is increasing. Content Delivery Network is proposed for efficient content delivery in an IP network. However, there are some issues, including over load to content servers, and so on. Content Centric Network attracts attention as a new generation network, which can solve these issues. However, in Content Centric Network anyone who knows the content ID would get the contents, therefore, it is difficult to deliver content only to permitted person. To solve this problem, we propose the group data access control method. The proposed method is evaluated confidentiality, integrity and availability, and performance. The proposed method is also evaluated its action by emulation program.