gebhart/patrepeval huggingface dataset:
A fuzzy logic based closed-loop control system for blood glucose level regulation in diabetics (2005)

In this study, a closed-loop system to control the plasma glucose level in patients with diabetes mellitus type 1 is proposed. This control scheme is based on fuzzy logic control theory to maintain a normoglycaemic average of 4.5 mmol 1(-1) and the normal conditions for free plasma insulin concentration in severe initial state; in particular, when the diabetic patient is subjected to a glucose meal disturbance or fluctuations in the measured glucose level due to error in the measuring instrument. The proposed controller has demonstrated superiority over other conventional controlling therapies. While fuzzy logic controllers have shown promising results in many fields, a comparative study is presented with well-known conventional controllers such as Proportional-Integral-Derivative (PID) and continuous insulin infusion control strategies. The simulated results, for the proposed controller, are presented and discussed.
Sol–gel encapsulation of metalloproteins for the development of optical biosensors for nitrogen monoxide and carbon monoxide (1995)

Three heme proteins; horse heart cytochrome c, myoglobin (Mb) and hemoglobin (Hb), have been encapsulated in a sol–gel matrix and investigated for their potential use as biological recognition centres for the biosensing of NO and CO. It has been shown that the encapsulation of these metalloproteins in sol–gels has no effect on the formation of nitrosyl or carbonyl complexes as observed using UV/VIS spectrophotometry. The metalloproteins in sol–gels could be readily reduced to the FeII state, using excess sodium dithionite, and re-oxidized to the FeIII state on the addition of excess potassium ferricyanide. The observed redox capability within sol–gels enabled formation of the nitrosyl complex in both the FeIII and FeII states of Mb and Hb and in the FeIII state of cytochrome c. The formation of these nitrosyl complexes was shown to be reversible. Similarly, the reversible formation of the carbonyl complex with Mb and Hb in the reduced form has been achieved. Titration of a saturated solution of CO with Hb contained in a sol–gel showed a linear increase in intensity of the α(568 nm) and β(540 nm) absorption bands of the metalloprotein until saturation of the iron centres of the Hb occurred. A similar increase in intensity of the α(562 nm) absorption band of cytochrome c was observed when a saturated solution of NO was titrated against this metalloprotein contained in a sol–gel. The results show that sol–gels can be readily used as a host for the encapsulation of cytochrome c, Mb and Hb and that these metalloproteins can be used as the biological recognition centre for the biosensing of NO and CO at the micromolar level.
The multicopper oxidase of <i>Pseudomonas aeruginosa</i> is a ferroxidase with a central role in iron acquisition (2002)

Recently it has been observed that multicopper oxidases are present in a number of microbial genomes, raising the question of their function in prokaryotes. Here we describe the analysis of an mco mutant from the opportunistic pathogen Pseudomonas aeruginosa. Unlike wild-type Pseudomonas aeruginosa, the mco mutant was unable to grow aerobically on minimal media with Fe(II) as sole iron source. In contrast, both the wild-type and mutant strain were able to grow either anaerobically via denitrification with Fe(II) or aerobically with Fe(III). Analysis of iron uptake showed that the mco mutant was impaired in Fe(II) uptake but unaffected in Fe(III) uptake. Purification and analysis of the MCO protein confirmed ferroxidase activity. Taken together, these data show that the mco gene encodes a multicopper oxidase that is involved in the oxidation of Fe(II) to Fe(III) subsequent to its acquisition by the cell. In view of the widespread distribution of the mco gene in bacteria, it is suggested that an iron acquisition mechanism involving multicopper oxidases may be an important and hitherto unrecognized feature of bacterial pathogenicity.
Clinical and psychological characteristics of the initial cohort of the Dominantly Inherited Alzheimer Network (DIAN). (2013)

Overall cognitive and personality deficits in very mild ADAD are similar to those seen in sporadic AD. Cognitive deficits also occur in asymptomatic mutation carriers who are closer to the expected time of dementia onset.
A Tale of Two Complications of Obesity: NASH and Hepatocellular Carcinoma (2019)

Nonalcoholic steatohepatitis (NASH) is the most common cause of chronic liver disease in developed countries and its incidence is rapidly increasing. Cirrhosis, and the dreaded complication of hepatocellular carcinoma (HCC), are the major drivers of morbidity and mortality in NASH. Conventional understanding has been that chronic liver damage leads to a cycle of cell death, regeneration and fibrosis during which HCC precursor cells undergo malignant transformation and lead to cancer initiation. This is supported by epidemiologic data which shows that cirrhosis precedes HCC in more than 90% of patients with several forms of chronic liver disease like hepatitis C and alcohol cirrhosis. But the link between fibrosis and carcinogenesis seems less definitive in patients with NASH as a sizeable proportion of NASH patients with HCC do not have significant underlying fibrosis. Several case reports and case series have pointed out this phenomenon of HCC arising in non-cirrhotic NASH (1), and a recent meta-analysis of 19 studies has shown that the prevalence of HCC in non-cirrhotic NASH was up to 38.0% (2). The mechanisms that contribute to the development of HCC in obesity in the absence of NASH and/or overt fibrosis or cirrhosis have remained unexplored. A possible mechanism to explain the role of obesity in the pathogenesis of HCC independent of NASH, was recently reported in a paper in Cell (3) .
Expression of a cell surface immobilization antigen during serotype transformation in Tetrahymena thermophila. (1985)

A temperature shift from 40 to 28 degrees C rapidly induced expression of a specific immobilization antigen at the cell surface in Tetrahymena thermophila. This transformation was inhibited by actinomycin D and cycloheximide but not by colchicine or cytochalasin B. The major surface antigen expressed at 28 degrees C in cells homozygous for the SerH3 allele was partially purified, and an antiserum against this preparation was raised in rabbits. Electrophoresis, immunoblot, and [35S]methionine incorporation studies are reported which support the conclusion that the H3 antigen is an acidic protein with an Mr of approximately 52,000 daltons. An induced synthesis of the H3 immobilization antigen was detected within 30 min after a shift from 40 to 28 degrees C. This protein appeared to be synthesized in the microsomal fraction and transferred without cleavage to the cell surface, where it was inserted first into nonciliated regions.
Renewable Hydrogen: Integration, Validation, and Demonstration (2008)

This paper is about producing hydrogen through the electrolysis of water and using the hydrogen in a fuel cell or internal combustion engine generator to produce electricity during times of peak demand, or as a transportation fuel.
Phase 2 trial of CPX-351, a fixed 5:1 molar ratio of cytarabine/daunorubicin, vs cytarabine/daunorubicin in older adults with untreated AML (2014)

CPX-351 is a liposomal formulation of cytarabine:daunorubicin designed to deliver synergistic drug ratios to leukemia cells. In this phase 2 study, newly diagnosed older acute myeloid leukemia (AML) patients were randomized 2:1 to first-line CPX-351 or 7+3 treatment. The goal was to determine efficacy and identify patient subgroups that may benefit from CPX-351 treatment. Response rate (complete remission + incomplete remission) was the primary end point, with event-free survival (EFS) and overall survival (OS) as secondary end points. The 126 patients entered were balanced for disease and patient-specific risk factors. Overall, CPX-351 produced higher response rates (66.7% vs 51.2%, P = .07), meeting predefined criteria for success (P < .1). Differences in EFS and OS were not statistically significant. A planned analysis of the secondary AML subgroup demonstrated an improved response rate (57.6% vs 31.6%, P = .06), and prolongation of EFS (hazard ratio [HR] = 0.59, P = .08) and OS (HR = 0.46, P = .01). Recovery from cytopenias was slower after CPX-351 (median days to absolute neutrophil count ≥1000: 36 vs 32; platelets >100 000: 37 vs 28) with more grade 3-4 infections but without increase in infection-related deaths (3.5% vs 7.3%) or 60-day mortality (4.7% vs 14.6%), indicating acceptable safety. These results suggest a clinical benefit with CPX-351, particularly among patients with secondary AML, and provide the rationale for a phase 3 trial currently underway in newly diagnosed secondary AML patients. This study is registered at Clinicaltrials.gov as #NCT00788892.
Dietary cod liver oil decreases arachidonic acid in rat gastric mucosa and increases stress‐induced gastric erosions (2000)

The purpose of this study was to examine the influence of long-term feeding of dietary fat rich in either n-3 or n-6 fatty acids on the availability of arachidonic acid (20:4n-6) in major phospholipids of gastric mucosa in rats. Three groups of male Wistar rats were fed either a standard diet, a cod liver oil-enriched diet (10% by weight), or a corn oil-enriched diet (10% by weight) for 8 mon. Dietary cod liver oil significantly reduced the level of 20:4n-6 in phosphatidylcholine (PC) and in phosphatidylethanolamine (PE) of gastric mucosa. The loss of 20:4n-6 was compensated for by eicosapentaenoic acid (20:5n-3) in PC, whereas the decrease in 20:4n-6 in PE corresponded to the increase in three n-3 fatty acids: 20:5n-3, docosapentaenoic acid (22:5n-3), and docosahexaenoic acid (22:6n-3). The level of 20:5n-3 was higher than the level of 22:6n-3 both in PC and PE of mucosa in rats fed cod liver oil. Diets supplemented with corn oil increased the level of 18:2n-6 but decreased the monoene fatty acids 16:1 and 18:1n-7 in PC but not in PE of gastric mucosa. The 20:4n-6 levels of both PC and PE were markedly reduced by dietary cod liver oil, to about one-third of control levels. Similar changes were also observed in the stomach wall. Gastric erosions were observed in all rats exposed to restriction stress, but this form of stress induced twice the number of erosions in rats fed fish oil compared to control rats or rats fed corn oil. We conclude that a diet rich in fish oil altered the balance between n-6 and n-3 fatty acids in major gastric mucosal phospholipids, markedly reduced the availability of 20:4n-6, and increased the incidence of gastric erosions induced by restriction or emotional stress.
Wavelength dependence of skin cancer induction by ultraviolet irradiation of albino hairless mice. (1993)

Information on the variation in carcinogenicity with wavelength is crucial in risk assessments for skin cancers induced by UV radiation. Until recently the wavelength (lambda) dependencies of other detrimental UV effects, such as sunburn, have been used as substitutes. Direct information on the lambda dependency can only be obtained from animal experiments. To this end we accumulated a large data set on skin tumors induced by chronic UV exposure of albino SKH:HR1 mice (14 different broadband UV sources and about 1100 mice); the data come from the Photobiology Unit of the former Skin and Cancer Hospital in Philadelphia and from the Department of Dermatology of the University of Utrecht. The lambda dependency was extracted from this data set (a statistically satisfactory description with chi 2 = 13.4, df = 7) and represented by the Skin Cancer Utrecht-Philadelphia action spectrum, i.e., a set of factors to weight the exposures at different wavelengths according to their respective effectiveness (inversely proportional to the daily exposure required for a median tumor induction time of 300 days). The fits obtained with other already available action spectra proved to be poor (chi 2 > 60, df = 11). The maximum effectiveness was found at 293 nm, and above 340 nm the effectiveness showed a shoulder at about 10(-4) of the maximum. A sensitivity analysis of the final solution for the lambda dependency showed a large margin of uncertainty above 340 nm and an information gap below 280 nm. The large variation in tumor responses in the present data set can be transformed to a coherent, common dose-response relationship by proper spectral weighting with this single action spectrum.
Plastic Behavior of Metals in the Strain-Hardening Range. Part I (1937)

The stress-strain relations for the cold working of the ductile metals are described for a group of cases assuming that the stresses under which they yield plastically depend only on the permanent parts of the strains, leaving out of the consideration the influence of the elasticity and of the speed of the plastic deformation. The behavior of the metals in the strain-hardening range is expressed by means of a strain-hardening function which connects the octahedral shearing stress with the octahedral unit shear. These variables are defined for strains of finite magnitude.
TransMedial All‐Inside Posterior Cruciate Ligament Reconstruction Using a Reinforced Tibial Inlay Graft (2013)

Surgical reconstruction of the posterior cruciate ligament (PCL) is technically demanding. Potential challenges include visualization of the tibial footprint and drilling of the tibial tunnel without damaging posterior neurovascular structures, as well as graft selection, deployment, tensioning, and fixation. We present a novel TransMedial all-inside arthroscopic technique (technique designed by A. J. Wilson with support from Arthrex) using a single hamstring tendon graft, fixed with adjustable cortical suspensory devices. The technique simplifies the difficult steps encountered during PCL reconstruction and is safe and reproducible. All arthroscopic viewing is accomplished from the lateral portal, and femoral socket preparation is performed from the medial side with specially contoured instruments, which allow accurate marking, measuring, and anatomic positioning of the graft. The quadrupled semitendinosus graft can be augmented with composite polymer tape for increased strength and initial stability. We use outside-in drilling to create retrograde femoral and tibial sockets. Cortical suspensory fixation on the tibial side can be supplemented with anchor fixation. We use an arthroscopic tibial inlay technique that better approximates native knee anatomy. This also avoids the "killer turn," a problem seen in transtibial PCL reconstruction techniques, which theoretically induces graft laxity due to abrasion with cyclic loading. This technique can be further adapted to allow a modified double-bundle or TriLink graft (technique designed by A. J. Wilson with support from Arthrex.).
Phosphorylation of the Platelet-derived Growth Factor Receptor-β and Epidermal Growth Factor Receptor by G Protein-coupled Receptor Kinase-2 (2002)

Accumulating evidence suggests that receptor protein-tyrosine kinases, like the platelet-derived growth factor receptor-beta (PDGFRbeta) and epidermal growth factor receptor (EGFR), may be desensitized by serine/threonine kinases. One such kinase, G protein-coupled receptor kinase-2 (GRK2), is known to mediate agonist-dependent phosphorylation and desensitization of multiple heptahelical receptors. In testing whether GRK2 could phosphorylate and desensitize the PDGFRbeta, we first found by phosphoamino acid analysis that cells expressing GRK2 could serine-phosphorylate the PDGFRbeta in an agonist-dependent manner. Augmentation or inhibition of GRK2 activity in cells, respectively, reduced or enhanced tyrosine phosphorylation of the PDGFRbeta but not the EGFR. Either overexpressed in cells or as a purified protein, GRK2 demonstrated agonist-promoted serine phosphorylation of the PDGFRbeta and, unexpectedly, the EGFR as well. Because GRK2 did not phosphorylate a kinase-dead (K634R) PDGFRbeta mutant, GRK2-mediated PDGFRbeta phosphorylation required receptor tyrosine kinase activity, as does PDGFRbeta ubiquitination. Agonist-induced ubiquitination of the PDGFRbeta, but not the EGFR, was enhanced in cells overexpressing GRK2. Nevertheless, GRK2 overexpression did not augment PDGFRbeta down-regulation. Like the vast majority of GRK2 substrates, the PDGFRbeta, but not the EGFR, activated heterotrimeric G proteins allosterically in membranes from cells expressing physiologic protein levels. We conclude that GRK2 can phosphorylate and desensitize the PDGFRbeta, perhaps through mechanisms related to receptor ubiquitination. Specificity of GRK2 for receptor protein-tyrosine kinases, expressed at physiologic levels, may be determined by the ability of these receptors to activate heterotrimeric G proteins, among other factors.
The role of phosphocreatine kinase in the motility of human spermatozoa supported by different metabolic substrates (1996)

In the spermatozoa of some species creatine kinase (CK: E.C. 2.7.3.2) is involved in shuttling energy, in the form of creatine phosphate, between the mid-piece mitochondria and flagellum. In this study, the effects of the CK inhibitor dinitrofluorobenzene (DNFB) on human sperm CK activity, motility and ATP concentrations were assessed with different energy substrates. There was a dose-dependent inhibition of CK activity by DNFB but inhibition was incomplete and there was no effect on the percentage of flagellating cells, irrespective of substrate. However, when lactate alone supported the cells DNFB decreased velocities and increased amplitude of head displacement (fewer progressively motile forms were observed), whereas ATP concentrations in spermatozoa were unaltered. Demembranated sperm models could be reactivated by ADP plus creatine phosphate, but not to the extent caused by ATP, and were able to be inhibited by myokinase inhibitors. Increased velocities, linearity (LIN) and beat cross frequency (BCF) were demonstrated for spermatozoa incubated with lactate, in contrast to glucose as sole energy source, and higher velocities and BCF were generated in the presence of both substrates. This suggests that the production of ATP by glycolysis and respiration are independent and complementary. CK is not obligatory for sperm motility but supplements energy provision under certain conditions.
Therapeutic Advantage of Hypoxic Cells in Tumors: A Theoretical Study (1991)

The presence of hypoxic cells in solid tumors has long been considered a problem in cancer treatment, particularly for radiation therapy but also for treatment with some anticancer drugs. Three general strategies are being actively explored to overcome the problem: oxygenating the tumor, sensitizing the hypoxic cells to radiation (or chemotherapy), or killing the hypoxic cells (with a hypoxic cell cytotoxin). In the present study, we have examined the impact of each of these three strategies on a standard radiation therapy regimen (30 doses of 2 Gy), using either of two major assumptions: full reoxygenation or no reoxygenation of the tumor cells. We demonstrate that a hypoxic cell cytotoxin can produce a level of tumor cell killing higher (by several orders of magnitude) than that produced by full oxygenation of a tumor or by use of an optimum hypoxic cell radiosensitizer, provided the cytotoxin kills more than approximately 50% of the hypoxic cells each time it is given. The only assumption that makes a difference is whether reoxygenation occurs: In the worst case (ie, no reoxygenation), the hypoxic cell cytotoxin performs only as well as an optimum radiosensitizer. The analysis shows that hypoxic cells in tumors can be a major therapeutic advantage. Therefore, we recommend that research efforts be concentrated on development of superior hypoxic cell cytotoxins rather than improved hypoxic cell radiosensitizers and that, in parallel, emphasis be placed on development of agents to increase hypoxia.
Active camera guided manipulation (2002)

A scheme that uses an active camera to guide object manipulation in an environment that has not been modeled is introduced. By actively moving camera to desired position with proper distance and viewing angle from objects of interest, it is possible to acquire good data for robot visual feedback control when locating objects. The environment is described in an object-centered coordinates system and is measured relatively in consecutive 2-D image spaces. This approach is flexible and efficient in manipulation, since it avoids complicated 3-D modeling and image processing is driven by tasks. It is shown that robot operation and camera motion are guided by visual feedback. The strategies to control this active guidance are studied.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Method to Encapsulate Molecular Cargo Within DNA Icosahedra (2013)

DNA self-assembly has yielded various polyhedra based on platonic solids. DNA polyhedra can act as nanocapsules by entrapping various molecular entities from solution and could possibly find use in targeted delivery within living systems. A key requirement for encapsulation is that the polyhedron should have maximal encapsulation volume while maintaining minimum pore size. It is well known that platonic solids possess maximal encapsulation volumes. We therefore constructed an icosahedron from DNA using a modular self-assembly strategy. We describe a method to determine the functionality of DNA polyhedra as nanocapsules by encapsulating different cargo such as gold nanoparticles and functional biomolecules like FITC dextran from solution within DNA icosahedra.
Boron and Phosphorus dopant activation in Germanium using laser annealing with and without preamorphization implant (2009)

In this work, we studied excimer laser annealing at 308 nm with 180 ns pulse duration on phosphorus and boron implanted in germanium, with or without pre-amorphization implant (PAI) and co-implant. Using an industrial tool, experimental results show that we can achieve electrical activation levels up to 1.2×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> for P implant in Ge, which is the highest level regarding the appropriate mobility model. With the B implanted samples, we obtained an electrical activation level higher than 1×10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">20</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> which is the better results obtained without PAI. Melt thresholds were determined to be 0.65 J/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in amorphized germanium (a-Ge) and 0.95 J/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in crystalline germanium (c-Ge). With P, the best activation was obtained after a complete melt of the amorphous layer and the amorphous / crystalline (a/c) interface, necessary to obtain a perfectly recrystallized layer. In the case of B, we found a better activation in the submelt regime compared the melt one, and no contribution on the electrical activation with PAI was observed.
Analogue write-precompensation scheme fordigital magnetic recording (1998)

Existing write-precompensation schemes for digital magnetic recording are partly digital and have a restricted memory span. The simple scheme presented here is fully analogue and has arbitrary memory span.