gebhart/patrepeval huggingface dataset:
ATP Regulation in Adult Rat Cardiomyocytes (2006)

The mechanisms that enable the heart to rapidly increase ATP supply in line with increased demand have not been fully elucidated. Here we used an adenoviral system to express the photoproteins luciferase and aequorin, targeted to the mitochondria or cytosol of adult cardiomyocytes, to investigate the interrelationship between ATP and Ca(2+) in these compartments. In neither compartment were changes in free [ATP] observed upon increased workload (addition of isoproterenol) in myocytes that were already beating. However, when myocytes were stimulated to beat rapidly from rest, in the presence of isoproterenol, a significant but transient drop in mitochondrial [ATP] ([ATP](m)) occurred (on average to 10% of the initial signal). Corresponding changes in cytosolic [ATP] ([ATP](c)) were much smaller (<5%), indicating that [ATP](c) was effectively buffered in this compartment. Although mitochondrial [Ca(2+)] ([Ca(2+)](m)) is an important regulator of respiratory chain activity and ATP production in other cells, the kinetics of mitochondrial Ca(2+) transport are controversial. Parallel experiments in cells expressing mitochondrial aequorin showed that the drop in [ATP](m) occurred over the same time scale as average [Ca(2+)](m) was increasing. Conversely, in the absence or presence of isoproterenol, clear beat-to-beat peaks in [Ca(2+)](m) were observed at 0.9 or 1.3 mum, respectively, concentrations similar to those observed in the cytosol. These results suggest that mitochondrial Ca(2+) transients occur during the contractile cycle and are translated into a time-averaged increase in mitochondrial ATP production that keeps pace with increased cytosolic demand.
The human XPC DNA repair gene: arrangement, splice site information content and influence of a single nucleotide polymorphism in a splice acceptor site on alternative splicing and function (2002)

XPC DNA repair gene mutations result in the cancer-prone disorder xeroderma pigmentosum. The XPC gene spans 33 kb and has 16 exons (82-882 bp) and 15 introns (0.08-5.4 kb). A 1.6 kb intron was found within exon 5. Sensitive real- time quantitative reverse transcription-polymerase chain reaction methods were developed to measure full-length XPC mRNA (the predominant form) and isoforms that skipped exons 4, 7 or 12. Exon 7 was skipped in approximately 0.07% of XPC mRNAs, consistent with the high information content of the exon 7 splice acceptor and donor sites (12.3 and 10.4 bits). In contrast, exon 4 was skipped in approximately 0.7% of the XPC mRNAs, consistent with the low information content of the exon 4 splice acceptor (-0.1 bits). A new common C/A single nucleotide polymorphism in the XPC intron 11 splice acceptor site (58% C in 97 normals) decreased its information content from 7.5 to 5.1 bits. Fibroblasts homozygous for A/A had significantly higher levels (approximately 2.6-fold) of the XPC mRNA isoform that skipped exon 12 than those homozygous for C/C. This abnormally spliced XPC mRNA isoform has diminished DNA repair function and may contribute to cancer susceptibility.
Smart Polymer Surfaces (2003)

A methodology is demonstrated for creating responsive polymer surfaces that can alter their wetting characteristics when exposed to a humid environment. For this, we take advantage of the surface partitioning of block copolymers at the polymer/air interface and utilize a hydrophilic group at the end of the surface-active block. When exposed to water vapor, the end group, hidden below the surface when in contact with dry air, presents itself to the surface thus significantly reducing the water contact angle. This function can be reversed and repeated again and again by successive exposures of the surface to dry and wet environment, respectively.
Short‐Chain Fatty Acids Induce Pro‐Inflammatory Cytokine Production Alone and in Combination with Toll‐Like Receptor Ligands (2011)

Our data suggest that SCFAs, especially when combined with specific TLR ligands, contribute to a pro-inflammatory milieu in the lower genital tract and help further our understanding of how BV affects susceptibility to microbial infections.
Mammalian Antifertility Agents. I. Derivatives of 2,3-Diphenylindenes<sup>1</sup> (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMammalian Antifertility Agents. I. Derivatives of 2,3-Diphenylindenes1Daniel Lednicer, John C. Babcock, Paul E. Marlatt, Stanley C. Lyster, and Gordon W. DuncanCite this: J. Med. Chem. 1965, 8, 1, 52–57Publication Date (Print):January 1, 1965Publication History Published online1 May 2002Published inissue 1 January 1965https://pubs.acs.org/doi/10.1021/jm00325a013https://doi.org/10.1021/jm00325a013research-articleACS PublicationsRequest reuse permissionsArticle Views166Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Biomechanically induced tissue reactions after Al<sub>2</sub>O<sub>3</sub>–ceramic hip joint replacement. Experimental and early clinical results (1976)

A biomechanical concept of interface maturation around cement-free implanted alumina-ceramic hip endoprostheses in sheep is explained. Provided there is rigid primary stability of the implant within the bone bed, hard tissue formation along the support areas of the implant is mainly dependent on implant shape and main local stress direction. Three subsequent phases of interface maturation can be distinguished, finally resulting in firm fixation of the implant by mechanical interlocking of supporting bone and ceramic. A new composite alumina-metal hip endoprosthesis for partial cement-free implantation (cup) is presented. The technique, postoperative care, and encouraging early results in 5 patients are demonstrated. A new ceramic femoral component for cement free implantation is proposed.
Alpha and beta subunits of the nicotinic acetylcholine receptor contain covalently bound lipid. (1984)

Labeling of the BC3H1 muscle-like cell line with [3H] palmitate, followed by immunoprecipitation of the acetylcholine receptor, indicated that the alpha and beta subunits of the receptor contain covalently bound fatty acid. After acid hydrolysis, fatty acid methyl esters could be recovered from the isolated [3H]palmitate-labeled alpha subunit. Treatment of differentiated BC3H1 cells with cerulenin, an inhibitor of fatty acid and sterol synthesis and fatty acid acylation of proteins, resulted in a 50% inhibition in expression of the acetylcholine receptor on the cell surface under conditions where there was minimal inhibition of protein synthesis. We conclude that this previously undetected post-translational modification may play a role in assembly and/or surface expression of the acetylcholine receptor.
Multicolor Luciferase Assay System: One-Step Monitoring of Multiple Gene Expressions with a Single Substrate (2005)

Reporter assays that use luciferase are widely employed for monitoring cellular events associated with gene expression. In general, firefly luciferase and Renilla luciferase are used for monitoring single gene expression. However, the expression of more than one gene cannot be monitored simultaneously by this system because one of the two reporting luciferases must be used as an internal control. We have developed a novel reporter assay system in which three luciferases that emit green, orange, and red light with a single substrate are used as reporter genes. The activities of the luciferases can be measured simultaneously and quantitatively with optical filters. This system enables us to simply and rapidly monitor multiple gene expressions in a one-step reaction.
Over‐production of IFN‐γ and IL‐12 in AhR‐null mice (2005)

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates toxicity of environmental pollutants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin. The exposure to AhR agonists results in profound suppression of cellular and humoral immune responses and compromises host to infectious disease. Therefore, to define the role of AhR in the immune response, spleen cells from ovalbumin (OVA)-immunized and naïve mice were removed and stimulated in vitro with either OVA or mitogen concanavalin-A (Con A), respectively. Proliferation, CD19+, F4/80+, CD4+ and CD8+ T cells expansion and cytokines production were measured in C57BL/6-AhR-/- mice (AhR-/-) and compared with immune response in similarly immunized age-matched wild type (AhR+/+) mice. In response to OVA immunization, AhR-/- mice had similar levels of serum OVA-specific IgG2a, IgG1, and IgG2b compared with AhR+/+ animals. However, AhR-/- mice showed splenomegalia and an increase in B cells. No changes were observed on proliferation and IL-4 secretion, although AhR-/- cells produced more IFN-gamma and IL-12 than AhR+/+ cells. Similar results were observed with Con A stimulation, a decrease on IL-5 and no change on IL-2 secretion were observed on AhR-/- cells compared with AhR+/+ cells in response to Con A stimulation. High levels of IFN-gamma mRNA were detected in AhR-/- lymphocytes, but IL-4 mRNA levels in AhR-/- cells were similar to those in AhR+/+ mice. These data suggest that AhR may play an important role in the normal development and function of immune system by down-regulating IFN-gamma and IL-12 expression.
A Balanced Approach to Tourniquet Use: Lessons Learned and Relearned (2006)

Welling, David R. USAF MC (RET), MD, FACS, FASCRS*; Burris, David G. USA MC, MD, FACS; Hutton, John E. USA MC (RET), MD, FACS; Minken, Stanley L. MD, FACS; Rich, Norman M. USA MC (RET), MD, FACS Author Information
A parallel vector-quantization processor eliminating redundant calculations for real-time motion picture compression (2000)

A parallel vector-quantization (VQ) processor has been developed aiming at real-time compression of motion pictures using a 0.35-/spl mu/m triple-metal CMOS technology. The chip employs a new search algorithm for VQ encoding to reduce hardware volume. As a result, the die area of the chip is decreased to only 33% of that of conventional fully parallel design. This chip can handle 2048 template vectors by a single chip and is applicable to real-time compression of 30 frames/s full-color VGA (640/spl times/480 pixels) images. The chip operates at 33 MHz with power dissipation of 790 mW under 2.5-V power supply. A real-time motion picture compression system is demonstrated as an application of the chip. This system can transmit five full-color QVGA (320/spl times/240 pixels) images per second below 64 kb/s.
Measurement of the cylinder pressure in combustion engines with a piezoresistive β-SiC-on-SOI pressure sensor (2002)

For measuring the cylinder pressure in combustion engines a high temperature pressure sensor has been developed. The sensor is made of a piezoresistive /spl beta/-SiC-on-SOI (SiCOI) sensor chip and a specially designed housing. The SiCOI sensor was characterized under static pressure up to 20 MPa. The sensitivity of the sensor at room temperature is approximately 1.9 mV/MPa and decreases to about 1.2 mV/MPa at 300/spl deg/C. For monitoring the dynamic cylinder pressure in the combustion chamber the SiCOI sensor was placed into the cylinder head of a gasoline engine. The measurements were performed at 1500 rpm under different loads, and for comparison a quartz pressure transducer from Kistier was used as a reference. The maximum pressure at partial load operation amounts to about 2.0 MPa. The difference between the calibrated SiCOI sensor and the reference sensor is significantly less than 0.1 MPa during the whole operation.
D/sup over/; an optimal on-line scheduling algorithm for overloaded real-time systems (2003)

An optimal online scheduling algorithm for overloaded systems is presented. It is optimal in the sense that it gives the best competitive factor possible relative to an offline (i.e., clairvoyant) scheduler. It also gives 100% of the value of a clairvoyant scheduler when the system is underloaded. In fact the performance guarantee of D/sup over/ is even stronger: D/sup over/ schedules to completion all tasks in underloaded periods and achieves at least 1/(1+ square root k)/sup 2/ of the value a clairvoyant algorithm can get during overloaded periods. The model accounts for different value densities and generalizes to soft deadlines.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Gas Lift Smart Flow Integrates Quality and Control Data for Diagnostic and Optimization in Real Time (2013)

Abstract Intelligent digital oilfield (iDOF) operations include the transfer, monitoring, visualization, analysis, and interpretation of realtime data. Enabling this process requires a significant investment in surface, subsurface, and well instrumentation and a sophisticated infrastructure for data transmission and visualization. This upgraded system can then transfer massive quantities of data. Automated intelligent workflows, referred to here as "smart flows", convert this raw data into real information at the right time. To increase efficiency and decrease downtime in operations, oil companies have invested heavily in digital oilfield infrastructure. An important part of the puzzle is transferring data from the wellsite to the office in real time and using that data to optimize production systems. The challenge is to extract real-time information from raw data at the right time to affect the management of the fields, which is the primary goal. North Kuwait’s Sabriyah field is operated using an intelligent digital oilfield operation by a major Middle Eastern oil company. Part of the intelligent digital oilfield project is a smart flow dedicated to monitoring, diagnostics, and optimization of gas lift (GL) wells. Leading-edge technologies—such as subsurface equipment and sensors, advanced diagnostics based on artificial intelligent agents, analysis of sensors signals, and automatically identifying GL optimum operating-conditions—are used to accomplish this goal. Using a steady-state nodal-analysis model combined with an artificial intelligent technique, the smart flow is designed to provide rapid diagnostics and optimization in real time, generating actions, such as decreasing and increasing the gas lift injection rate and choke setting. The ultimate benefit is timely detection of those signals that foretell unexpected well downtime or equipment failure. The paper describes the main functionalities of the GL smart flow as a powerful optimization tool capable of providing an interactive monitoring system to assist the company in managing its GL-operated wells.
On the complexity of partially-flow-sensitive alias analysis (2008)

We introduce the notion of a partially -flow-sensitive analysis based on the number of read and write operations that are guaranteed to be analyzed in a sequential manner. We study the complexity of partially-flow-sensitive alias analysis and show that precise alias analysis with a very limited flow-sensitivity is as hard as precise flow-sensitive alias analysis, both when dynamic memory allocation is allowed, as well as in the absence of dynamic memory allocation.
Gene Expression Profiling for Molecular Characterization of Inflammatory Breast Cancer and Prediction of Response to Chemotherapy (2004)

Inflammatory breast cancer (IBC) is a rare but aggressive form of breast cancer with a 5-year survival limited to approximately 40%. Diagnosis, based on clinical and/or pathological criteria, may be difficult. Optimal systemic neoadjuvant therapy and accurate predictors of pathological response have yet to be defined for increasing response rate and survival. Using DNA microarrrays containing approximately 8,000 genes, we profiled breast cancer samples from 81 patients, including 37 with IBC and 44 with noninflammatory breast cancer (NIBC). Global unsupervised hierarchical clustering was able to some extent to distinguish IBC and NIBC cases and revealed subclasses of IBC. Supervised analysis identified a 109-gene set the expression of which discriminated IBC from NIBC samples. This molecular signature was validated in an independent series of 26 samples, with an overall performance accuracy of 85%. Discriminator genes were associated with various cellular processes possibly related to the aggressiveness of IBC, including signal transduction, cell motility, adhesion, and angiogenesis. A similar approach, with leave-one-out cross-validation, identified an 85-gene set that divided IBC patients with significantly different pathological complete response rate (70% in one group and 0% in the other group). These results show the potential of gene expression profiling to contribute to a better understanding of IBC, and to provide new diagnostic and predictive factors for IBC, as well as for potential therapeutic targets.
Symmetrized local co-registration optimization for anomalous change detection (2009)

The goal of anomalous change detection (ACD) is to identify what unusual changes have occurred in a scene, based on two images of the scene taken at different times and under different conditions. The actual anomalous changes need to be distinguished from the incidental differences that occur throughout the imagery, and one of the most common and confounding of these incidental differences is due to the misregistration of the images, due to limitations of the registration pre-processing applied to the image pair. We propose a general method to compensate for residual misregistration in any ACD algorithm which constructs an estimate of the degree of "anomalousness" for every pixel in the image pair. The method computes a modified misregistration-insensitive anomalousness by making local re-registration adjustments to minimize the local anomalousness. In this paper we describe a symmetrized version of our initial algorithm, and find significant performance improvements in the anomalous change detection ROC curves for a number of real and synthetic data sets.
Chicago Symphony Orchestra: Soloists of the Orchestra III (2010)

Chicago Symphony Orchestra: of the III. Chicago Symphony Orchestra, various conductors and soloists. Chicago Symphony CSO CD07-2 (2 CDs). (www.cso.org, Click on Shop Symphony Store). This is Volume 21 in a comprehensive series of recordings of Chicago Symphony performances recorded by the CSO's radio ally, WFMT, released on the CSO's own label. The CSO was founded by Theodore Thomas in 1891 with the notion that everyone in the orchestra should be a star performer in their own right and therefore able to step up to the plate and take the lead spot in a concerted work when need be. While this policy hasn't stopped the CSO from taking on prominent touring artists as an integral part of its season, it insures that the orchestra is not absolutely dependent on such artists to insure top flight performances of concerted works. The Soloists of the Orchestra series provides a little face time with some of the CSO's talented principals, who elected to choose the steady work of being a section leader over opting for the unpredictable vagaries of a touring artist's life. The first disc deals mainly with wind players: trumpeter Adolph Herseth, flautists Walfrid Kujala and Donald Peck, clarinetist Larry Combs and oboist Alex Klein. The second disc concentrates on string players: violinists Samuel Magad and Victor Aitay, cellists Frank Miller and John Sharp, the latter being heard in Pierre Boulez' Messagesquisse, scored for seven cellos and therefore encompassing much of the orchestra's cello section. The standout performance among these two generous discs is a stunning reading of Miklos Rozsa's Sinfonia Concertante for violin, cello and orchestra, Op. 29, dating from 1966 and featuring Hungarian-born violinist and (then) associate concertmaster Victor Aitay with legendary cellist Frank Miller. Miller had spent 15 years under the baton of Arturo Toscanini in the NBC Symphony before arriving in Chicago. They appeared as joint soloists only in this one work, which was given twice; this is the earlier occasion. Miller is memorialized in 1986 through the inclusion of a rare pre-concert talk given by Sir Georg Solti and a run-through of the Nimrod variation from Elgar's Enigma Variations. It's all very touching, although one wonders if Miller's memory might have been better served with a short selection devoted to his own playing; perhaps such a selection was not available for inclusion. Miller was a representative of the grand old manner of classical music making, steeped in romantic tradition; Boulez' Messagesquisse, which follows Solti's tribute straightaway and is conducted not by Boulez himself, but by Daniel Barenboim, is in immediate, stark contrast to the Elgar and makes one wonder if we are better off by virtue of moving on from artists such as Miller. As a short ensemble piece, Messagesquisse doesn't really provide much of an insight into the capabilities of cellist John Sharp; on the other hand, Samuel Magad's reading of the Korngold Violin Concerto is strong and dedicated, even if the 1994 recording seems a little less full bodied than most other things in this collection. …
Capturing the activity factor: impact on volume. (1993)

In addition to Patient Classification System's information, nurse managers can use activity tools to develop and justify budget needs. A basic accounting-type workflow analysis format, which goes beyond the "Midnight Census," accurately details a unit's patient volume and productive hours.
Retarded low‐dose doxycycline for EGFR or MEK inhibitor–induced papulopustular rash (2014)

Rld-doxycyline can improve EGFR-TKi- and MEKi-induced rash severity. Its effectiveness appears to be inferior to doxycycline 200 mg/day in more severe cases and in EGFR-mab-induced rash, but due to the advantageous side-effect profile, rld-doxycycline should be considered as a possible treatment option for papulopustular rash. Prospective, randomized trials are necessary to confirm these findings.
A novel design of a feedback-controlled optical microphone for aeroacoustics research (2010)

This study constitutes a proof of concept of a new feedback-controlled optical microphone for potential use in phased 'beam-forming' arrays utilized in aeroacoustics research. In the new microphone design, a fiber-optic lever sensor is employed as a means for measuring the center displacement of a stretched thin membrane caused by incident acoustic pressure. The membrane is constructed from polyvinylidene-fluoride which exhibits piezoelectric properties allowing actuation of the membrane in a feedback system to nullify the optically detected deflection. The feedback provision was used to actively modify sensor parameters, most notably membrane stiffness, resonant frequency and damping. Testing of a prototype microphone was performed using a plane wave tube calibrator. Using feedback control, the fundamental resonant frequency of the prototype capsule was increased from 3.61 to 5.1 kHz, a 41% increase. Also, feedback was used for dc attenuation, equivalent to 'stiffening' of the microphone membrane. The results demonstrate that feedback control is an effective method for improving the microphone's transient response, as well as for 'self-tuning' and matching of microphone parameters in sensing arrays.