gebhart/patrepeval huggingface dataset:
Intratumoral peptide injection enhances tumor cell antigenicity recognized by cytotoxic T lymphocytes: a potential option for improvement in antigen-specific cancer immunotherapy (2012)

Antigen-specific cancer immunotherapy is a promising strategy for improving cancer treatment. Recently, many tumor-associated antigens and their epitopes recognized by cytotoxic T lymphocytes (CTLs) have been identified. However, the density of endogenously presented antigen-derived peptides on tumor cells is generally sparse, resulting in the inability of antigen-specific CTLs to work effectively. We hypothesize that increasing the density of an antigen-derived peptide would enhance antigen-specific cancer immunotherapy. Here, we demonstrated that intratumoral peptide injection leads to additional peptide loading onto major histocompatibility complex class I molecules of tumor cells, enhancing tumor cell recognition by antigen-specific CTLs. In in vitro studies, human leukocyte antigen (HLA)-A*02:01-restricted glypican-3144-152 (FVGEFFTDV) and cytomegalovirus495-503 (NLVPMVATV) peptide-specific CTLs showed strong activity against all peptide-pulsed cell lines, regardless of whether the tumor cells expressed the antigen. In in vivo studies using immunodeficient mice, glypican-3144-152 and cytomegalovirus495-503 peptides injected into a solid mass were loaded onto HLA class I molecules of tumor cells. In a peptide vaccine model and an adoptive cell transfer model using C57BL/6 mice, intratumoral injection of ovalbumin257-264 peptide (SIINFEKL) was effective for tumor growth inhibition and survival against ovalbumin-negative tumors without adverse reactions. Moreover, we demonstrated an antigen-spreading effect that occurred after intratumoral peptide injection. Intratumoral peptide injection enhances tumor cell antigenicity and may be a useful option for improvement in antigen-specific cancer immunotherapy against solid tumors.
Constitutive expression of the nifA gene activates associative nitrogen fixation of Enterobacter gergoviae 57-7, an opportunistic endophytic diazotroph (2007)

This study and our previous studies suggest that manipulation of the promoter of the nifA gene in a nitrogen-fixing bacterium having a high colonization competence is a practical and promising approach to achieve a stable associative nitrogen fixation for cereals.
Immunostimulatory Oligonucleotides Inhibit Colonic Proinflammatory Cytokine Production in Ulcerative Colitis (2006)

Only certain classes of ISS-ODNs inhibit the enhanced TNF-alpha and IL-1beta generated ex vivo by inflamed colonic mucosa of patients with UC. The effect of ISS-ODNs is mediated by triggering of TLR-9. These results suggest a potential therapeutic value for ISS-ODNs in UC.
A performance evaluation model for a two port interferometer SAR-MTI (2002)

Moving targets are displaced in azimuth on a SAR map. A separate SAR-MTI (moving target imaging) process is therefore used to detect and relocate moving targets. A model for evaluating the effects of noise and clutter on the detection and relocation performances of a two port interferometer SAR MTI is presented.
Process Planning Knowledge Discovery Based on CAPP Database for Mechanical Manufacturing Enterprise (2008)

Knowledge discovery in database have been attracting a significant amount of research, industry attention in recent years. Process planning knowledge (PPK) is one of the most important knowledge in mechanical manufacturing enterprise. The traditional method of turning data into knowledge relies on manual analysis and interpretation. This paper analyzes the source and composing of process planning knowledge and state of arts on process planning discovery in mechanical manufacturing enterprise. On the basis of the widely application of computer aided process planning (CAPP) system in mechanical manufacturing enterprise, the concept of process planning knowledge discovery (PPKD) is proposed based on process planning databases. This paper provides an overview CAPP database of the field, clarifying how PPKD in CAPP database are related both to each other and to related fields, the technology architecture of process planning knowledge discovery is founded based on object-oriented model-driven technology, and the process planning knowledge discovery script is designed. Elementary application research in typical process knowledge discovery is described in detail. The technology of PPKD in this paper has been tested in an aeronautical manufacturing enterprise to support the automatically knowledge acquisition in CAPP system. Good application effect has been revealed.
The Relationship between Risk-Taking, Sensation-Seeking, and the Tourist Behavior of Young Adults: A Cross-Cultural Study (2004)

This study analyzed the effects of the combined psychological characteristics of risk-taking and sensation seeking on the travel behavior and preferred tourist activities of young adults on leisure trips. The results of this cross-cultural study, which was conducted among 1,429 students at 11 universities located in 11 different countries, found that respondents with high combined risk-taking and sensation seeking (RSS) scores differed significantly in their travel behavior, mode of destination choice, preferred tourist activities and demographics, from those who had low RSS scores. The study also discovered a significant difference between nationalities on RSS scores.
Optimized stereo matching in binocular three-dimensional measurement system using structured light (2014)

In this paper, we develop an optimized stereo-matching method used in an active binocular three-dimensional measurement system. A traditional dense stereo-matching algorithm is time consuming due to a long search range and the high complexity of a similarity evaluation. We project a binary fringe pattern in combination with a series of N binary band limited patterns. In order to prune the search range, we execute an initial matching before exhaustive matching and evaluate a similarity measure using logical comparison instead of a complicated floating-point operation. Finally, an accurate point cloud can be obtained by triangulation methods and subpixel interpolation. The experiment results verify the computational efficiency and matching accuracy of the method.
Evaluation of Salmonella Serotype Distributions from Commercial Broiler Hatcheries and Grower Houses (1999)

By conventional trayliner (hatcheries) and drag swab assembly (broiler houses) culture methods, the isolation distribution of Salmonella serotypes from five commercial broiler hatcheries (three sample times) and 13 broiler farms (eight sample times) was evaluated. A total of 11 different Salmonella serotypes were isolated from hatcheries, with Salmonella heidelberg (9/30) and Salmonella kentucky (6/30) accounting for 50% of the total isolations. Of 700 chick paperpad trayliners sampled, regardless of lot (breeder flock source) or hatchery, 12% were positive for Salmonella. When 10 individual trayliners were cultured from individual lots (same breeder flock source), Salmonella was detected in 24/57 lots (42%). Multiple serotypes were simultaneously isolated from the same lot on three occasions (6%). Of the 21 lots that were serially sampled, the Salmonella serotype detected was different within lots eight times (38%) on at least one occasion of two or more sampling times. Of the 196 individual broiler houses sampled, 44 were positive for Salmonella (42%). Twelve different serotypes were isolated from broiler houses during this study. The serotypes isolated most frequently were S. heidelberg (34/94) and S. kentucky (22/94). These two serotypes accounted for 59.6% (56/94) of the total broiler house isolations. Of the 38 houses that were serially sampled, two or more serotypes were detected in the same broiler house on 20 occasions (53%). Of the 38 serially sampled houses (four or more times), a consistent Salmonella serotype was detected in five houses (13%). In only 5 of the 38 (13%) serially sampled houses did we fail to detect Salmonella on four or more samplings. No significant difference in Salmonella isolation frequency was observed between poultry houses using new or used litter. These data support previous findings indicating that paratyphoid Salmonella serotypes are prevalent in some broiler hatcheries and houses. Further, the observation of multiple serotypes simultaneously and serially isolated from the same breeder hatchery lots suggests that breeder flocks may be infected with more than one serotype, possibly providing a source for multiple serotype infections in progeny grower flocks.
Identification of a factor IX/IXa binding protein on the endothelial cell surface. (1987)

Endothelium provides a specific binding site for Factor IX/IXa which can propagate activation of coagulation by promoting Factor IXa-VIII-mediated activation of Factor X. In this report the endothelial cell Factor IX/IXa binding site has been identified and the coagulant function of the receptor blocked. Studies using [3H]Factor IX derivatized with the photoaffinity labeling agent N-succinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate (SANPAH) and cultured bovine endothelial cells demonstrated cross-linking to a trypsin-sensitive cell surface protein of Mr approximately equal to 140,000. Immunoprecipitation of metabolically labeled endothelium with Factor IX derivatized with the cleavable cross-linking agent N-succinimidyl(4-azidophenyl)-1,3'-dithiopropionate and antibody to Factor IX demonstrated the endothelial cell origin of the Mr 140,000 cell surface protein. Blockade of the Factor IX/IXa binding protein by covalently linking SANPAH-5-dimethylaminonaphthalene-1-sulfonyl-Glu-Gly-Arg-Factor IXa or SANPAH-Factor IX prevented both specific Factor IXa binding and effective Factor IXa-VIII-mediated activation of Factor X on endothelium. Following extraction of endothelium with detergents, Factor IX/IXa binding activity was solubilized and could be assayed using a polyvinyl chloride plate binding assay. Western blots of cell extracts demonstrated binding of 125I-Factor IX at Mr approximately equal to 140,000 which was blocked by excess Factor IX, but not antisera to Factor VIII, von Willebrand factor, alpha 2-macroglobulin, or epidermal growth factor receptor. These data indicate that endothelium provides a distinct binding site for Factor IX/IXa consisting, at least in part, of a membrane protein which can modulate the coagulant activity of Factor IXa on the cell surface.
Adaptive structures- concepts and prospects. (1993)

Vibration and stabilty phenomena cause performance resictions in our technical world. An innovative technology - that of adaptive structures - seems to be a new development step that clearly exceeds the actual existing technical limits. The DLR is increasing its efforts in adaptive structures research within the project ARES (Actively Reacting Flexible Structures). The concept of ARES is essentially proposed here.
Data flow analysis as model checking (1990)

The paper develops the idea that modal logic provides an appropriate framework for the specification of data flow analysis (DFA) algorithms as soon as programs are represented as models of the logic. This can be exploited to construct a DFA-<strong>generator</strong> that generates efficient DFA-algorithms from modal specifications by partially evaluating a specific model checker wrt the specifying modal formula. Moreover, the use of a modal logic as specification language for DFA-algorithms supports the compositional development of specifications and structured proofs of properties of DFA-algorithms. These ideas are applied to the problem of determining optimal computation points within flow graphs.
High-efficiency micro-optical color filter for liquid-crystal projection system applications (2000)

High-efficiency color filters composed of a microprism array, optical interference color filters, and a microlens array light compressor were developed to increase the optical throughput of liquid-crystal projection systems. The new devices utilize the energy of whole spectra by taking full advantage of a light compressor and interference dichroic filters to distribute the energy of spectra to the respective color pixel area. Thus high-efficiency micro-optical color filters allow efficient utilization of the energy of incident light and maximize the optical throughput of the projection system.
Analysis of Apoptosis by Flow Cytometry (2003)

Based on morphological, biochemical, and molecular criteria two distinct modes of cell death can be recognized: necrosis and apoptosis (1–5). Necrosis generally results from enzymatic digestion of the cells and denaturation of its proteins as the response to gross injury. An early event of necrosis is swelling of cell mitochondria, followed by rupture of the plasma membrane and release of the cytoplasmic contents. The necrotic cells is eosinophilic, and its nucleus becomes either pycnotic or karyorrhectic, or it may disappear in the process of karyolysis. Fully developed myocardial infarct, encephalomalacia, or caseous changes in tuberculosis are examples of necrosis. The process triggers an inflammatory reaction in the surrounding tissues, which often results in scar formation.
Characterization of the mRNA Capping Apparatus of the Microsporidian Parasite Encephalitozoon cuniculi (2002)

A scheme of eukaryotic phylogeny has been suggested based on the structure and physical linkage of the enzymes that catalyze mRNA cap formation. Here we show that the intracellular parasite Encephalitozoon cuniculi encodes a complete mRNA capping apparatus consisting of separate triphosphatase (EcCet1), guanylyltransferase (EcCeg1), and methyltransferase (Ecm1) enzymes, which we characterize biochemically and genetically. The triphosphatase EcCet1 belongs to a metal-dependent phosphohydrolase family that includes the triphosphatase components of the capping apparatus of fungi, DNA viruses, and the malaria parasite Plasmodium falciparum. These enzymes are structurally and mechanistically unrelated to the metal-independent cysteine phosphatase-type RNA triphosphatases found in metazoans and plants. Our findings support the proposed evolutionary connection between microsporidia and fungi, and they place fungi and protozoa in a common lineage distinct from that of metazoans and plants. RNA triphosphatase presents an attractive target for antiprotozoal/antifungal drug development.
Mixed, Neutral-Charged, Platinum-Platinum and Platinum-Palladium Macrocyclic Tetranuclear Complexes (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMixed, Neutral-Charged, Platinum-Platinum and Platinum-Palladium Macrocyclic Tetranuclear ComplexesPeter J. Stang and Jeffery A. WhitefordCite this: Organometallics 1994, 13, 10, 3776–3777Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://pubs.acs.org/doi/10.1021/om00022a010https://doi.org/10.1021/om00022a010research-articleACS PublicationsRequest reuse permissionsArticle Views285Altmetric-Citations69LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
ECONOMICS FAVOR GTL PROJECTS WITH CONDENSATE COPRODUCTION (1998)

A moderate scale (20,000 b/d) gas-to-liquids (GTL) project can have viable economics if condensate revenues are credited to the project. Many reserves contain valuable condensates and other liquids as well as natural gas. Without such coproduction, near-to-medium term economics are challenging. A few projects of this size will pave the way for larger projects that benefit from economies of scale. The larger projects will have viable economics without the benefits of liquid coproduction. Liquid coproduction gives the larger projects even stronger economics. These economics and further technology developments create strong prospects for a GTL industry. The most difficult challenge is taking the first steps. Flexibility will be needed on the part of host governments with regard to feed-gas pricing, the allocation of revenues from coproduced liquids, and tax and royalty questions. Project sponsors may have to accept greater risks and lower returns than they ideally wish. The prize for success, however, is substantial. The first part of this two-part series reviewed the development of GTL technology and the status of existing and proposed GTL projects. This second part considers the opportunities for new GTL projects based on economics. The available of suitable gas resources and the drivers for GTL developmentsmore » are discussed first.« less
Chondroitinase <scp>ABC</scp> promotes compensatory sprouting of the intact corticospinal tract and recovery of forelimb function following unilateral pyramidotomy in adult mice (2012)

Chondroitin sulphate proteoglycans (CSPGs) are extracellular matrix molecules whose inhibitory activity is attenuated by the enzyme chondroitinase ABC (ChABC). Here we assess whether CSPG degradation can promote compensatory sprouting of the intact corticospinal tract (CST) following unilateral injury and restore function to the denervated forelimb. Adult C57BL/6 mice underwent unilateral pyramidotomy and treatment with either ChABC or a vehicle control. Significant impairments in forepaw symmetry were observed following pyramidotomy, with injured mice preferentially using their intact paw during spontaneous vertical exploration of a cylinder. No recovery on this task was observed in vehicle-treated mice. However, ChABC-treated mice showed a marked recovery of function, with forelimb symmetry fully restored by 5 weeks post-injury. Functional recovery was associated with robust sprouting of the uninjured CST, with numerous axons observed crossing the midline in the brainstem and spinal cord and terminating in denervated grey matter. CST fibres in the denervated side of the spinal cord following ChABC treatment were closely associated with the synaptic marker vGlut1. Immunohistochemical assessment of chondroitin-4-sulphate revealed that CSPGs were heavily digested around lamina X, alongside midline crossing axons and in grey matter regions where sprouting axons and reduced peri-neuronal net staining was observed. Thus, we demonstrate that CSPG degradation promotes midline crossing and reinnervation of denervated target regions by intact CST axons and leads to restored function in the denervated forepaw. Enhancing compensatory sprouting using ChABC provides a route to restore function that could be applied to disorders such as spinal cord injury and stroke.
Proposal for Ultralow Loss Hollow-Core Plastic Bragg Fiber With Cobweb-Structured Cladding for Terahertz Waveguiding (2007)

The transmission losses of a terahertz (THz) wave in a hollow-core Bragg fiber with cobweb cladding are analyzed. The numerical results show that the losses in the wavelength range of 70-1000 mum are below 8.5times10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-5</sup> dB/cm. The lowest loss of 9.03times10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-6 </sup> dB/cm or 2.08times10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-6</sup> cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> is obtained for Fiber A at 90mum. The losses in this new class of Bragg fibers are reduced to far lower than those of other fibers for a THz wave reported so far in the literature
Mapping of the binding interfaces of the proteins of the bacterial phosphotransferase system, HPr and IIAglc (1993)

Enzyme IIAglc and HPr are central regulatory and phosphocarrier proteins of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) of bacteria. During phosphoryl transfer from phosphoenolpyruvate to glucose, phosphate is transferred from HPr to enzyme IIAglc. In order to characterize the binding interfaces of the two proteins during phosphate transfer, 15N-edited and 15N-filtered NMR experiments have been recorded for the complex of enzyme IIAglc and HPr from Bacillus subtilis. Uniformly 15N-labeled enzyme IIAglc and nonlabeled HPr were used in these studies. Residues which undergo significant chemical shift changes upon complex formation have been identified for both proteins. The binding interfaces of the two proteins, suggested by the observed chemical shift changes, involve predominantly hydrophobic surfaces near the active site His-15 of HPr and the phosphoryl acceptor His-83 of IIAglc.
Reactive ion etching of aluminum and aluminum alloys in an rf plasma containing halogen species (1978)

An etching method capable of producing vertical-walled high-resolution patterns in aluminum and aluminum alloy films is described. The process consists of rf sputter etching in a plasma containing ion species, which react with the metal to form volatile or easily sputtered compounds. The presence of reactive species greatly enhances the etch rate, while the electric field maintains the directionality inherent in the sputtering process. Halogen ion species, as obtained in rf plasmas containing a partial pressure of Cl2, Br2, HCl, HBr, or CCl4, were used to produce reactive ion etching of Al. The etch rate in a CCl4 plasma at a power input of 0.6 W/cm2 is as high as 5000 Å/min. Variations in reaction rate with rf power, reactant concentration, reactant flow rate, temperature, gas pressure, batch size, and residual gas contamination are discussed. Etch rate data for various materials found suitable for masking are also presented.
A molecular inventory of human pancreatic islets: sequence analysis of 1000 cDNA clones (1993)

The islets of Langerhans play a central role in glucose homeostasis by secreting the polypeptide hormones insulin and glucagon. They are comprised primarily of four endocrine cell types: insulin-secreting beta-cells which represent about 70% of the cells in the islet along with smaller number of cells secreting glucagon, somatostatin and pancreatic polypeptide. Diabetes mellitus results from the specific loss or dysfunction of the beta-cells. Because of the central role of the islets of Langerhans in the regulation of glucose homeostasis, we are preparing a database of genes expressed in this tissue. One thousand cDNA clones randomly isolated from a human pancreatic islet library were partially sequenced yielding 280 kilobases of sequence. Database searches indicated that 397 of the cDNAs represented known human genes or human homologs of genes identified in other species and a further 58 sequences corresponded to expressed sequence tags identified in other tissues or cells (contamination by exocrine pancreatic tissue was estimated to be less than 10%). 545 of the cDNAs were not related to any other sequences in the databases. The islet cDNA collection provides a unique source of genes for genetic studies of diabetes as well as for molecular studies of islet function in normal and diabetic states.