gebhart/patrepeval huggingface dataset:
Determination of Optimal Conditions for Thawing and Diluting Cell-Bound CVI 988 Marek's Disease Vaccine and Stability of the Diluted Vaccine (2007)

The cell-associated vaccine strain CVI 988, which is the active component of several commercial Marek's disease vaccines, normally is frozen and stored in liquid nitrogen. In order to ascertain good efficacy of the vaccine, it is crucial that the right procedures are followed for thawing and diluting of the virus. In the study presented here, ampoules containing the frozen product were taken from storage in liquid nitrogen and were thawed in a water bath at 27 C, which is similar to a lukewarm bath, and in a water bath at 37 C, with and without agitation. The effect of the thawing procedure on the live virus titer of the vaccine was investigated. Samples of thawed vaccine were diluted in diluent with different temperatures, and live virus titers were determined directly after dilution and after incubation of the diluted vaccine at different temperatures. The results show that directly after thawing in the water baths at 27 C and 37 C, with or without agitation, the live virus titers for CVI 988 were all in the same range. After incubation of the thawed virus at both temperatures for 15 min, the live virus titers were still in the same range. Decreases in live virus titers were observed after incubation for 4 hr. Live virus titration of the vaccine in diluent in different temperatures revealed that the highest titers were found with diluent at a temperature of 30 C to 37 C and the lowest titers in diluent at a temperature of 5 C. In addition, a combination product containing cell-associated CVI 988 and cell-associated herpesvirus of turkeys (HVT) was tested. For this combination product, the titers for HVT also were highest in diluent with a high temperature (i.e., 37 C), whereas the titers for CVI were highest in diluent at a temperature of 22 C. Both strains had relatively low titers in diluent at 5 C. After incubation of the diluted vaccine at the various temperatures for several hours, again, live virus titrations were done. Live virus titers were most stable with diluent at temperatures of 22 C. Vaccine virus diluted in diluent at 37 C could be stabilized by placing the diluted vaccine at 5 C directly after diluting. After evaluation of these data, the following is recommended. For thawing of the vaccine, a water bath at approximately 27 C, which is similar to a lukewarm bath, is preferred. For diluting the vaccine, diluent should be used at a temperature of 22 C or higher. If diluted in diluent at temperatures higher than 22 C, the diluted vaccine should be stored under cooling in order to avoid titer losses.
Aerodynamic and Thermodynamic Effects of Coolant Injection on Axial Compressors (1963)

Summary A study has been made of the effects of inlet coolant injection upon axial compressor performance, using the results of tests on turboshaft engines. It is shown that evaporation of the coolant changes the stage work distribution as well as the ideal compression work and that these effects may be estimated by elementary thermodynamic methods. Simplified prediction procedures are suggested and compared with experimental results.
Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli (2009)

GcvB positively regulates acid resistance by affecting RpoS expression. These data advance our understanding of the sncRNA regulatory network involved in modulating acid resistance in E. coli.
Sensitivity and positive predictive value of implantable intrathoracic impedance monitoring as a predictor of heart failure hospitalizations: the SENSE-HF trial (2011)

An intrathoracic impedance-derived fluid index had low sensitivity and PPV in the early period after implantation of a device in chronic HF patients. Sensitivity improved within the first 6 months after implant. Further studies are needed to assess the place of this monitoring technology in the clinical management of patients with HF.
Slider-disk interactions during the load-unload process (1990)

The issue of slider-disk contact during the loading process is investigated. The effects of initial altitude, pitch, and roll are studied by using the LDV (laser Doppler vibrometer). The relative displacement of the slider with respect to the disk and the change of pitch and roll during the loading process are obtained for the 3380-type slider and the thin-film disk. The effect of the loading location of the disk is studied. LDV and AE (acoustic emission) measurements are carried out to detect possible slider-disk contacts during the loading process. The AE signal shows some bursts which have the slider ringing-frequency components. But by comparing the AE signal with the LDV results before and after lubricating the gap between the pivot and suspension, the sources of the AE bursts are found to be the normal contact and slip between the pivot and suspension. It is therefore concluded that carrying out the load-unload process of the positive pressure slider using a mechanism which actuates the middle part of the suspension is a suitable solution to high stiction and friction problems that can develop in the contact-start-stop process.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A near real-time, parallel and distributed adaptive object detection and retraining framework based on AdaBoost algorithm (2015)

Object detection (e.g., face detection) using supervised learning often requires extensive training, resulting in long execution times. If the system requires retraining to accommodate a missed detection, waiting several hours or even days in some cases before the system is ready, may not be acceptable in practical implementations. This paper presents a generalized object detection framework such that the system can efficiently adapt to misclassified data and be retrained within a few minutes. The methodology developed here is based on the popular AdaBoost algorithm for object detection. To reduce the learning time in object detection, we develop a highly efficient, parallel, and distributed AdaBoost algorithm that is able to achieve a training execution time of only 1.4 seconds per feature on 25 workstations. Further, we incorporate this parallel object detection algorithm into an adaptive framework such that a much smaller, optimized training subset is used to yield high detection rates while further reducing the retraining execution time. We demonstrate the usefulness of our adaptive framework on face and car detection.
Construction of a modified mini-Tn5 lacZY non-antibiotic marker cassette: ecological evaluation of a lacZY marked Pseudomonas strain in the sugarbeet rhizosphere (1996)

In order to monitor the fate of genetically manipulated fluorescent pseudomonads following release into the environment, a lacZY transposable cassette, lacking antibiotic resistance genes, was constructed using a pUT suicide plasmidj delivery system. The resulting plasmid, pUTLacZY, can be easily used to generate lacZY marked pseudomonads without having to use antibiotic resistance determinants. The lacZY transposon generates random, stable transcriptional/translationjl fusions on integration into the target genome. Pseudomonas fluorescens strain F113 was marked with lacZY and was unaltered with respect to ecological fitness in the rhizosphere. Although lateral gene transfer of the chromosomally integrated lacZY marker could be detected in vitro, it was not detected in rhizosphere microcosms.
A multimodal emotional communication based humans-robots interaction system (2016)

A multimodal emotional communication based humans-robots interaction (MEC-HRI) system is proposed, which includes multiple emotional communication modes such as speech, facial expression, and gesture. The robots in MEC-HRI system could recognize humans' emotions and respond to humans in accordance with their emotions. Firstly, MEC-HRI system acquires the multimodal emotional cues from sensors, e.g., microphone, Kinect, eye tracker, EEG. Secondly, emotion recognition based on single modal/multimodal cues is carried out. Thirdly, robots respond to humans by facial expresion, speech, gesture, etc. Four scenarios, i.e., tour guiding, game entertainment, family services and scenairo simulation, are performed in the expriment, by which smooth communication between humans and robots is realized. In prospect, the MEC-HRI system would be applied in serving robots for smart house, education, hospital, restaurant, and so on.
Dissimilar Metal Joining of 2024 and 7075 Aluminium Alloys to Titanium Alloys by Friction Stir Welding (2011)

The friction stir welding (FSW) process is a solid-state joining process and the joining temperature is lower than that used in the fusion welding processes. Therefore, for dissimilar metal welding, FSW is considered to offer several advantages over fusion welding. The present work investigated the weldability of duralumin and titanium alloys using friction stir welding. The aluminum plates used in this work were 2024-T3 and 7075-T651, and the titanium plates used were pure titanium and Ti-6Al-4V. The average tensile strength of the Ti/2024 FSW joints was 311 MPa, and the tensile strength of the Ti/2024 joint was higher than that of the Ti/7075 FSW joint when the joining conditions were the same. A mixed region of Ti alloy and Al alloy was observed at the joint interface, and the joints mainly fractured at this region, where there was an intermetallic compound layer. In this region, a TiAl3 intermetallic compound was detected by XRD. Therefore, it can be understood that this TiAl3 intermetallic compound affects the tensile strength of butt joints.
Endogenous Abscisic Acid and Indole-3-Acetic Acid and Somatic Embryogenesis in Cultured Leaf Explants of <i>Pennisetum purpureum</i> Schum. (1987)

Effects of application in vivo of glyphosate, fluridone, and paclobutrazol to glasshouse-grown donor plants of Pennisetum purpureum Schum. on endogenous levels of abscisic acid (ABA) and indole-3-acetic acid (IAA) in young leaves and on somatic embryogenesis in cultured leaf explants were studied. Treatment of plants with glyphosate (100 milligrams per liter) resulted in elevated levels of endogenous ABA and IAA in young leaves. In contrast, paclobutrazol (50% active ingredient; 200 milligrams per liter) did not alter the endogenous levels of ABA and IAA. Fluridone (100 milligrams per liter) markedly inhibited synthesis of ABA and leaf explants from fluridone-treated plants lost the capacity for somatic embryogenesis. Explants from glyphosate- or paclobutrazol-treated plants did not show any reduction in embryogenic capacity when compared with untreated control plants. Glyphosate and fluridone were also incorporated into the culture media at various concentrations (0 to 20 milligrams per liter) to study their effects in vitro on somatic embryogenesis in leaf explants from untreated, field-grown plants. Glyphosate was inhibitory to somatic embryogenesis but only at concentrations above 5 milligrams per liter. Fluridone inhibited somatic embryogenesis at all concentrations tested. Inhibition of somatic embryogenesis by fluridone, by either in vivo or in vitro application, could be overcome partially by (+/-)-ABA added to the culture medium. Exogenous application of (+/-)-ABA enhanced somatic embryogenesis and reduced the formation of nonembryogenic callus. Application of IAA or gibberellic acid (GA(3); >5 milligrams per liter) was inhibitory to somatic embryogenesis. These results indicate that endogenous ABA is one of the important factors controlling the embryogenic capacity of leaf explants in Napier grass.
Cozart(R) RapiScan Oral Fluid Drug Testing System: An Evaluation of Sensitivity, Specificity, and Efficiency for Cocaine Detection Compared with ELISA and GC-MS Following Controlled Cocaine Administration (2003)

Oral fluid has become a widely accepted alternative matrix for drugs of abuse detection. Immunoassays have been developed for on-site testing of cocaine and metabolites in oral fluid. The performance of the Cozart RapiScan Oral Fluid Drug Testing System (CRS) was evaluated in comparison with Cozart Microplate Enzyme Immunoassay Cocaine Oral Fluid Kit (COC ELISA) and gas chromatography-mass spectrometry (GC-MS) at several screening and confirmation cutoffs, including those proposed by SAMHSA and those currently in use in the U.K. Oral fluid samples (n = 1271) were collected prior to and following controlled clinical cocaine administration. CRS provides a qualitative screen at a preset cutoff of 30 microg/L. Sensitivity, specificity, and efficiency for CRS (30 microg/L) as compared with COC ELISA with a cutoff of 30 microg/L were 92.1%, 91.8%, and 92.0%. The comparison of CRS (30 microg/L) with the 8-mg/L proposed SAMHSA confirmation cutoffs for cocaine and/or benzoylecgonine exhibited a sensitivity of 82.7%, a specificity of 94.5%, and an efficiency of 87.6%. For this study, an alternative CRS cutoff of 20 microg/L was also evaluated. Performance characteristics of CRS (20 microg/L) at the proposed SAMHSA confirmation cutoffs were 89.9%, 89.7%, and 89.8%, respectively. At cutoffs in use in the U.K., 30- micro g/L CRS screen and 15- microg/L GC-MS cutoffs for cocaine, benzoylecgonine, and/or ecgonine methyl ester sensitivity, specificity, and efficiency were 89.4%, 92.2%, and 90.7%, respectively. Cozart RapiScan had performance similar to the COC ELISA assay for the detection of cocaine exposure and suitable sensitivity and specificity at the proposed SAMHSA cutoffs.
Vascular endothelial growth factor-induced angiogenic gene therapy in patients with peripheral artery disease (2004)

This phase 1 clinical trial tested the safety of intramuscular gene transfer by using naked plasmid DNA encoding the gene for VEGF, and analyzed the potential therapeutic benefits in patients with severe peripheral arterial disease (PAD). This study was an open-labeled, dose- escalating, single-center trial on nine male patients with severe debilitating PAD who had not responded to conventional therapy. Seven had Buerger's disease and two had arteriosclerosis obliterans. Plasmid DNA (pCK) containing human VEGF165 was given by eight intramuscular injections in and around the area in need of new blood vessels. The study evaluated three escalating total doses (2, 4, and 8 mug of pCK- VEGF165), with half of each total dose given four weeks apart. The follow-up duration was nine months. The gene injections were well tolerated without significant side effects or laboratory abnormalities related to gene transfer. Three patients showed transient edema in their extremities. Ischemic pain of the affected limb was relieved or improved markedly in six of seven patients. Ischemic ulcers healed or improved in four of six patients. The mean ankle-brachial index (ABI) improved significantly. Six of nine patients showed an increase in collateral vessels around the injection sites demonstrated by digital subtraction angiography. However, there was no relationship between the degree of ABI improvement and the dose given. Mean plasma levels of VEGF did not increase significantly. In conclusion, intramuscular injections of pCK- VEGF165 can be performed safely to induce therapeutic angiogenesis in patients with severe PAD.
c-Myb is an essential downstream target for homeobox-mediated transformation of hematopoietic cells (2006)

Abdominal-type HoxA genes in combination with Meis1 are well-documented on-cogenes in various leukemias but it is unclear how they exert their transforming function. Here we used a system of conditional transformation by an inducible mixed lineage leukemia-eleven-nineteen leukemia (MLL-ENL) oncoprotein to overexpress Hoxa9 and Meis1 in primary hematopoietic cells. Arrays identified c-Myb and a c-Myb target (Gstm1) among the genes with the strongest response to Hoxa9/Meis1. c-Myb overexpression was verified by Northern blot and quantitative reverse transcription-polymerase chain reaction (RT-PCR). Also MLL-ENL activated c-Myb through up-regulation of Hoxa9 and Meis1. Consequently, short-term suppression of c-Myb by small inhibitory RNA (siRNA) efficiently inhibited transformation by MLL-ENL but did not impair transformation by transcription factor E2A-hepatic leukemia factor (E2A-HLF). The anti c-Myb siRNA effect was abrogated by coexpression of a c-Myb derivative with a mutated siRNA target site. The introduction of a dominant-negative c-Myb mutant had a similar but weaker effect on MLL-ENL-mediated transformation. Hematopoietic precursors from mice homozygous for a hypo-morphic c-Myb allele were more severely affected and could be transformed neither by MLL-ENL nor by E2A-HLF. Ectopic expression of c-Myb induced a differentiation block but c-Myb alone was not transforming in a replating assay similar to Hoxa9/Meis1. These results suggest that c-Myb is essential but not sufficient for Hoxa9/Meis1 mediated transformation.
A Novel DAC Based Switching Power Amplifier for Polar Transmitter (2006)

A novel switching power amplifier based on the concept of digital to analog converter (DAC) is presented for polar transmitter architecture. The novel idea in this amplifier is to generate a current proportional to the amplitude modulation signal and the power control bits. The current is then up-converted to the frequency of interest using switching transistors. In this paper, we demonstrate that the performance of the proposed circuit is superior compare to the existing power amplifiers designed for polar transmitter. The measurement results show maximum output power of 27.8dBm with power efficiency of 34%. Moreover, the amplifier exhibits amplitude modulation bandwidth of 4.2MHz and 62dB power control dynamic range. The circuit is fabricated in CMOS 0.18mum process with 3.3V power supply
Suppression of tumorigenicity in somatic cell hybrids (1980)

Cell hybrids between nontumorigenic human diploid and tumorigenic heteroploid cells of the D98AH2 strain initially contain a complete or nearly complete chromosome complement of both parental lines. Of 35 such independent isolates all failed to form tumors when inoculated at 146 different sites in <i>nude</i> mice, even at cell doses 40 fold greater than those at which D98AH2 cells produce tumors. This agrees with observations of others in similar intraspecific systems. These hybrids remained nontumorigenic after subcloning and prolonged culture. However, they had not lost their tumorigenic potential since tumorigenic isolates could be recovered at a low incidence either by selecting in semisolid medium or by backselecting in 6-thioguanine supplemented medium. These tumorigenic isolates had lost chromosomes, primarily those of the diploid parent, suggesting that these chromosomes convey the suppressor information. Only specific chromosomes can carry such information, since other isolates with equally low chromosome numbers were not tumorigenic. A series of cell phenotypes often found in tumorigenic cells, such as infinite life span, anchorage independence, loss of contact inhibition, decreased fibronectin production, and ability to grow at low serum concentration, were found to segregate independently of tumorigenicity, clearly indicating that they are under separate control and that they are not cell properties that by themselves, or in concert, are essential for the tumorigenic phenotype. This agrees with observations of others. However, there was some indication that these characters may potentiate tumorigenicity.
Revised structures of soyasapogenols A,B,and E,oleanene-sapogenols from soybean. Structures of soyasaponins I,II,and III. (1982)

The structures of soyasapogenols A, B, and E, three of five hitherto isolated sapogenols of soybean (Glycine max Merrill), were re-investigated. Based on the chemical and X-ray analyses, it has been shown that the structures of soyasapogenols A, B, and E should be partly revised respectively from 1', 2', and 3'to 1, 2, and 3 and consequently the structures of soyasaponins I, II, and III are expressed as 6, 7, and 8.
Manufacture of concentrated Pyrethrum Extract (1932)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTManufacture of concentrated Pyrethrum ExtractC. B. Gnadinger, C. S. Corl, and Minneapolis MinnCite this: Ind. Eng. Chem. 1932, 24, 9, 988–991Publication Date (Print):September 1, 1932Publication History Published online1 May 2002Published inissue 1 September 1932https://pubs.acs.org/doi/10.1021/ie50273a005https://doi.org/10.1021/ie50273a005research-articleACS PublicationsRequest reuse permissionsArticle Views44Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
A unified diode model for circuit simulation (1997)

A new commercially available diode model is described. This unified model is capable of simulating the widest range of diode technologies of any presently available. The emphasis of this paper is on describing the model's extensive features and flexibility in the different domains of operation and is of particular interest in power applications.
Genetic Manipulations of Fatty Acid Metabolism in β-Cells Are Associated With Dysregulated Insulin Secretion (2002)

Triacylglyceride (TG) accumulation in pancreatic beta-cells is associated with impaired insulin secretion, which is called lipotoxicity. To gain a better understanding of the pathophysiology of lipotoxicity, we generated three models of dysregulated fatty acid metabolism in beta-cells. The overexpression of sterol regulatory element binding protein-1c induced lipogenic genes and TG accumulation. Under these conditions, we observed a decrease in glucose oxidation and upregulation of uncoupling protein-2, which might be causally related to the decreased glucose-stimulated insulin secretion. The overexpression of AMP-activated protein kinase was accompanied by decreased lipogenesis, increased fatty acid oxidation, and decreased glucose oxidation; insulin secretions to glucose and depolarization stimuli were decreased, probably because of the decrease in glucose oxidation and cellular insulin content. It was notable that the secretory response to palmitate was blunted, which would suggest a role of the fatty acid synthesis pathway, but not its oxidative pathway in palmitate-stimulated insulin secretion. Finally, we studied islets of PPAR-gamma(+/-) mice that had increased insulin sensitivity and low TG content in white adipose tissue, skeletal muscle, and liver. On a high-fat diet, glucose-stimulated insulin secretion was decreased in association with increased TG content in the islets, which might be mediated through the elevated serum free fatty acid levels and their passive transport into beta-cells. These results revealed some aspects about the mechanisms by which alterations of fatty acid metabolism affect beta-cell functions.