gebhart/patrepeval huggingface dataset:
Lack of association between the 5HT 2A receptor polymorphisms ( T102C) and unipolar affective disorder in a multicentic European study (2003)

We report here a case-control association study with T102C polymorphism in the serotonin 2A receptor gene (HTR2A) in patients affected by unipolar affective disorder (UPAD) and in controls. A total of 284 subjects were genotyped (142 UPAD and 142 controls). All subjects were interviewed using standard diagnostic interviews and matched. A homogenous population of unipolar patients with suicidal attempt was identified. Conditional logistic regression was applied. No association of the HTR2A polymorphism was found in the overall sample of 142 UPAD-control pairs regarding allele and genotype frequencies (P=0.36 and P=0.52 respectively) and homo-heterozygote distributions (P=0.91). This study confirms, in a multicentric European sample, the earlier observations that the T102C HTR2A polymorphism is not associated with UPAD. Nevertheless, a type 2 statistical error cannot be excluded. Therefore, to exclude the implication of HTR2A in UPAD, this result must be replicated in larger samples and in other populations using the transmission disequilibrium test and different polymorphisms around HTR2A.
Temperature and Shear Susceptibility of a Nonpetroleum Binder as a Pavement Material (2010)

Most bituminous binders used for pavement materials are derived from fossil fuels, specifically crude petroleum. Nowadays, technical and economic prospects exist in using biorenewable resources to produce biobinders. Biobinders can be used in three ways to decrease the demand for crude petroleum–derived bituminous binders: direct alternative binder (100% replacement), bitumen extender (25% to 75% bitumen replacement), and bitumen modifier (<10% bitumen replacement). Applicability of developing biobinders from oakwood-based bio-oils to be used as a direct alternative has been investigated through studying the rheological properties. Temperature and shear rate (rate of loading) of biobinders and modified biobinders play major roles in changing the viscosity of bio-oils. The rheological properties of oakwood bio-oils have been investigated and compared with those of bitumen binders, to study the applicability of producing biobinders. Temperature and shear susceptibilities have been studied through measuring the viscosity of the bio-oils. Results reveal that the relationship between the viscosity of bio-oils and temperature and shear rates are log linear–like bitumen binders. In addition, temperature is the main contributor to the viscosity of the bio-oils in comparison with shear rate. Important is that the viscosity temperature susceptibility values for the bio-oils in comparison with bitumen blends indicate that bio-oils are more susceptible to temperature. Moreover, the addition of polymer modifiers leads to a change in temperature ranges of the bio-oils. In conclusion, the rheological properties of oakwood bio-oils are similar to and comparable with bitumen binders, and they represent a viable renewable alternative to petroleum-derived asphalt binders.
A new Monte Carlo approach to structure solution from powder data (1996)

Unlike Monte Carlo procedures for structure solution reported thus far we report a new approach which not only allows random movements of individual atoms and rigid-body fragments comprising an asymmetric unit but offers a means of changing internal configuration of stereochemically constrained constituent groups. The last option can be crucial when flexible atomic assemblies like chains. sheets and other moieties are considered.
Novel expression of human chorionic gonadotropin/luteinizing hormone receptor gene in brain. (1993)

LH from anterior pituitary and hCG from placenta bind to a common receptor in gonadal and nongonadal reproductive tissues. There have been numerous examples suggesting that the brain may also contain hCG/LH receptors, yet there has been no evidence for their existence so far. We now demonstrate by reverse transcription-nested polymerase chain reaction and northern blotting that the rat brain contains hCG/LH receptor mRNA. A major receptor transcript of 2.6 kilobases and minor transcripts of 1.8 and 4.4 kilobases were found. Western immunoblotting, ligand blotting, and covalent receptor cross-linking studies have shown that rat brain also contains an 80-kilodalton receptor protein that can bind hCG and hLH, but not hFSH. Rat testis used as a positive control showed a higher abundance of multiple transcripts and an 80-kilodalton receptor protein that can bind [125I]hCG. Rat liver used as a negative control did not contain any receptor transcripts or protein. In situ hybridization, dot blotting, immunocytochemistry, and topical autoradiography have revealed that hCG/LH receptors are present in rat hippocampus; dentate gyrus; hypothalamus; cerebellum; choroid plexus; ependymal cells of third, fourth, and lateral ventricles; cortex; brainstem; bovine hypothalamus; and human area postrema. These novel findings could potentially explain numerous previous observations and suggest new possibilities concerning the regulation of brain functions by hCG and LH.
Induction of apoptosis in macrophages by <i>Pseudomonas aeruginosa</i> azurin: tumour‐suppressor protein p53 and reactive oxygen species, but not redox activity, as critical elements in cytotoxicity (2003)

Azurin is a copper-containing protein involved in electron transfer during denitrification. We reported recently that purified azurin demonstrates cytotoxicity to macrophages by forming a complex with the tumour-suppressor protein p53, thereby stabilizing it and enhancing its function as an inducer of proapoptotic activity (Yamada, T., Goto, M., Punj, V., Zaborina, O., Kimbara, K., Das Gupta, T. K., and Chakrabarty, A. M. 2002, Infect Immun70: 7054-7062). It is, however, not known whether the oxidoreductase (redox) activity of azurin or the involvement of copper is important for its cytotoxicity. We have isolated apo-azurin devoid of copper and site-directed mutants that are redox negative because of either replacement of a cysteine residue (Cys-112) involved in co-ordination with copper or mutational replacement of two methionine residues (Met-44 and Met-64) that are present in the hydrophobic patch of azurin and allow interaction of azurin with its redox partner cytochrome c551. We demonstrate that, although the wild type (wt) and the Cys-112 Asp mutant azurin can form complexes with the tumour-suppressor protein p53 and generate high levels of reactive oxygen species (ROS), the redox-negative Met-44LysMet-64Glu mutant azurin is defective in complex formation with p53, generates low levels of ROS and lacks appreciable cytotoxicity towards macrophages. Thus, complex formation with p53 and ROS generation, rather than azurin redox activity, are important in the cytotoxic action of azurin towards macrophages.
<title>Parallel Processing Algorithms And Architectures For Real-Time Signal Processing</title> (1982)

This paper discusses parallel computation algorithms and architectures for real-time signal processing, with emphasis on progress toward the hardware realization of a numerical linear algebra library of functions. The objective is to utilize VLSI/VHSIC technology in parallel architectures to provide a real-time equivalent of the LINPACK/EISPACK capabilities.
Preliminary comparison of material charging properties using single-energy and multienergy electron beams (1981)

Results of tests designed to compare the charging properties of spacecraft material under a monoenergetic beam and under a continuously distributed beam are summarized. In the test setup the electron source was mounted in the top of a cylindrical vacuum chamber. A sheet of 5-mil-thick Kapton was placed on an insulated metal substrate at the bottom of the vacuum chamber. The dc current arriving on the substrate was measured and a field meter measured the potential of the test sample. A retarding potential analyzer measured the energy spectrum of the incident electron beam. With the monoenergetic beam, the sample charged to 12 kV and electrical discharges occurred. With the multi-energy spectra the samples charged to only 6 kV and 4 kV, and no discharger were observed. In these experiments, no effort was made to duplicate the spectra occurring in space. The tests were intended to simply to compare spacecraft material charging properties using monoenergetic and continuous multi-energy beams.
Ground-based all-sky mid-infrared and visible imagery for purposes of characterizing cloud properties (2014)

Abstract. This paper describes the All Sky Infrared Visible Analyzer (ASIVA), a multi-purpose visible and infrared sky imaging and analysis instrument whose primary function is to provide radiometrically calibrated imagery in the mid-infrared (mid-IR) atmospheric window. This functionality enables the determination of diurnal fractional sky cover and estimates of sky/cloud temperature from which one can derive estimates of sky/cloud emissivity and cloud height. This paper describes the calibration methods and performance of the ASIVA instrument with particular emphasis on data products being developed for the meteorological community. Data presented here were collected during the Solmirus' ASIVA campaign conducted at the Atmospheric Radiation Measurement (ARM) Southern Great Plains (SGP) Climate Research Facility from 21 May to 27 July 2009. The purpose of this campaign was to determine the efficacy of IR technology in providing reliable nighttime sky cover data. Significant progress has been made in the analysis of the campaign data over the past several years and the ASIVA has proven to be an excellent instrument for determining sky cover as well as the potential for determining sky/cloud temperature, sky/cloud emissivity, precipitable water vapor (PWV), and ultimately cloud height.
Distributed Hypertext Resource Discovery Through Examples (1999)

We describe the architecture of a hypertext resource discovery system using a relational database. Such a system can answer questions that combine page contents, meta-data, and hyperlink structure in powerful ways, such as "find the number of links from an environmental protection page to a page about oil and natural gas over the last year." A key problem in populating the database in such a system is to discover web resources related to the topics involved in such queries. We argue that that a keyword-based "find similar" search based on a giant all-purpose crawler is neither necessary nor adequate for resource discovery. Instead we exploit the properties that pages tend to cite pages with related topics, and given that a page u cites a page about a desired topic, it is very likely that u cites additional desirable pages. We exploit these properties by using a crawler controlled by two hypertext mining programs: (1) a classifier that evaluates the relevance of a region of the web to the user's interest (2) a distiller that evaluates a page as an access point for a large neighborhood of relevant pages. Our implementation uses IBM's Universal Database, not only for robust data storage, but also for integrating the computations of the classifier and distiller into the database. This results in significant increase in I/O efficiency: a factor of ten for the classifier and a factor of three for the distiller. In addition, ad-hoc SQL queries can be used to monitor the crawler, and dynamically change crawling strategies. We report on experiments to establish that our system is efficient, effective, and robust.
Toxicology of the Eye (1986)

At a time when many new, promising pharmaceutical products are available for the treatment of eye disease, and when complexities of brand new information can confound and bewilder, it is a relief to be presented with an orderly volume, indeed, a marvelous encyclopedia of the effects of drugs and chemicals on the eye, that is complete and repleat with nuggets of clinically useful information. We have long been in the debt of Professor W. M. Grant for his dedication to scholarship in ophthalmology and ophthalmic science. Now that Dr Grant has produced his third edition of<i>Toxicology of the Eye</i>, we again are grateful to him for his continued painstaking devotion to accuracy, documentation, and just plain good clinical judgment. An example of his accuracy can be seen on page 471 in the description of hair dyes. Examples of his documentation are evident throughout the text in the wonderful yet
Mibefradil prevents L-NAME-exacerbated nephrosclerosis in spontaneously hypertensive rats (1999)

Our data show that mibefradil prevented the development of hypertension and proteinuria, renal functional impairment and nephrosclerosis, and also improved animal survival. The renal protective effects of mibefradil were at least equivalent to those of an ACE inhibitor in this animal model of chronic renal failure.
Large-scale assembly of carbon nanotube-based flexible circuits for DNA sensors (2008)

We present a simple but efficient method to prepare carbon nanotube (CNT)-based flexible devices embedded in polymer substrates. In this strategy, a methyl-terminated self-assembled monolayer is first coated on a solid substrate as a release layer, and CNT-network devices fabricated on it are directly transferred into a poly(dimethylsiloxane) (PDMS) mold, resulting in flexible CNT-network devices embedded in PDMS. The embedded circuits exhibit stable operation even after significant bending. We also propose Raman spectroscopy as a powerful tool to remotely characterize the CNT-network device structures covered by a polymer layer. As a proof of concept, we demonstrate DNA sensors utilizing the fabricated CNT-network devices.
Purification and characterization of DNA polymerase from the archaebacterium<i>Sulfolobus acidocaldarius</i> (1985)

DNA polymerase has been purified about 25,000-fold from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. On SDS-PAGE the enzyme was observed to have a molecular weight of 100 kDa and to be about 90% pure. The native molecular weight was 108 kDa indicating that the enzyme is composed of a single polypeptide. Activity gel analysis showed an active polypeptide of about 100 kDa. Under conditions promoting proteolysis this polypeptide was degraded to a slightly smaller form of 98 kDa. The enzyme has been characterized in respect to optimal assay conditions, template specificity, sensitivity to inhibitors and associated nuclease activities. The high temperature optimum of 65 degrees C should be emphasized. No substantial similarities have been found with other prokaryotic and eukaryotic DNA polymerases, although the enzyme bears certain resemblances to prokaryotic non-replicative polymerases.
Enhanced Activity of Cu/ZnO/C Catalysts Prepared by Cold Plasma for CO<sub>2</sub> Hydrogenation to Methanol (2020)

An activated carbon-supported Cu/ZnO catalyst for CO2 hydrogenation to methanol has been prepared by the facile plasma decomposition of a Cu–Zn precursor at moderate temperature. The Cu/ZnO/C catalyst prepared by cold plasma featured smaller size and higher dispersion than that prepared by traditional calcination. The low operation temperature plays an important role in this. The catalysts are characterized using a multitechnique approach. X-ray photoelectron spectroscopy results indicate that cold plasma can introduce specific O-containing groups on the carbon surface. It also facilitates the dispersion of active components and H spillover. H2 temperature-programmed reduction profiles and transmission electron microscopy images show that the interaction between Cu, ZnO, and carbon in the Cu/ZnO/C catalyst prepared by cold plasma was stronger than that in the catalyst prepared by calcination. The properties are beneficial to the CO2 hydrogenation reaction. The catalytic performance of the catalysts was tested in a fixed-bed reactor. The Cu/ZnO/C catalyst prepared by cold plasma showed an obvious increase in CO2 conversion, methanol selectivity, and productivity in the catalytic evaluation of catalysts. Moreover, it obtained a remarkable space-time yield of 92.5 gmethanol·kgcat–1·h–1 at 260 °C that is approximately 1.5 times that of Cu/ZnO/C prepared by calcination.
Full-size Testing And Analysis of X120 Linepipe (2004)

A new high-strength steel with a specified minimum yield strength of 120 ksi has been developed for linepipe applications. Because existing specifications were not originally intended for the X120, and no service experience exists for this grade, fullsize pipe tests and finite element analyses were conducted to evaluate structural performance. The full-size testing included burst, pure bending, collapse, bending + collapse, ring expansion, curved wide plate and crack arrest tests. In addition to these tests, bending trials were performed on the X120 using commercial pipeline construction equipment to verify cold deformability. This paper presents the burst and fracture arrest studies. Results from burst tests and analyses demonstrate sufficient pressure containment capacity. For crack arrest, full-size tests were conducted to evaluate the intrinsic arrestability of the material and the effectiveness of crack arrestors. Although the X120 pipe did not have sufficient toughness for intrinsic arrest for the prescribed test conditions, tight-fitting sleeve crack arrestors were shown to be effective in stopping propagating fractures.
Synthesis and cytotoxic activities of a series of novel<i>N</i>-methyl-bisindolylmaleimide amide derivatives (2014)

A novel series of N-methyl-bisindolylmaleimides were synthesized and evaluated for their inhibitory activities against nine tumor cell lines. Some of the compounds showed an interesting activity against the tested cell lines. The most potent compounds 5e and 5j displayed antiproliferative activity with 50% inhibitory concentration values in the μM range against some tested cell lines.
Condensations at the Methyl Group of Ethyl Acetoacetate by Means of Potassium Amide or Sodium Hydride<sup>1</sup> (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCondensations at the Methyl Group of Ethyl Acetoacetate by Means of Potassium Amide or Sodium Hydride1James F. Wolfe, Thomas M. Harris, and Charles R. HauserCite this: J. Org. Chem. 1964, 29, 11, 3249–3252Publication Date (Print):November 1, 1964Publication History Published online1 May 2002Published inissue 1 November 1964https://pubs.acs.org/doi/10.1021/jo01034a029https://doi.org/10.1021/jo01034a029research-articleACS PublicationsRequest reuse permissionsArticle Views729Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Synthesis, characterization and catalytic CO oxidation studies over Ni 1-x Cu x Mn 2 O 4 (1999)

Ni , .• Cu.Mn20 4 (x = 0.0, 0.3, 0.5, 0.7, 1.0) have been prepared by co-precipitation method and characterized by XRD, AAS and BET method. The solid state studies such as electrical resistivity, magnetic susceptibility and electron spin reso­ nance(ESR) have been carried out and are attempted to correlate with the catalytic activity of the compounds. The model reaction of CO oxidation has been undertaken to evaluate the catalytic activity and kinetics. Higher activity of intermediate compositions is observed due to the phenomena of synergism.
Peptide-Derivatized Shell-Cross-Linked Nanoparticles. 2. Biocompatibility Evaluation (2004)

The conjugation of the protein transduction domain (PTD) from the HIV-1 Tat protein to shell cross-linked (SCK) nanoparticles is a method to facilitate cell surface binding and transduction. In the previous report, the preparation, derivatization, and characterization of peptide-functionalized SCK nanoparticles were reported in detail. Following assembly, the constructs were evaluated in vitro and in vivo to obtain a preliminary biocompatibility assessment. The effects of SCK exposure on cell viability were evaluated using a metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and a fluorescent apoptosis assay. Furthermore, stages of apoptosis were quantified by flow cytometry. Although higher levels of peptide functionalization resulted in decreased metabolic function as measured by MTT assay, significant apoptosis was not observed below 500 mg/L for all the samples. To evaluate the potential immunogenic response of the peptide-derivatized constructs, a real-time polymerase chain reaction (RT-PCR) system that allows for the in vitro analysis and quantification of the cellular inflammatory responses tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL1-beta) was utilized. The inflammatory response to the peptide-functionalized SCK nanoparticles as measured by RT-PCR show statistically significant increases in the levels of both TNF-alpha and IL1-beta relative to tissue culture polystyrene (TCPS). However, the measured cytokine levels did not preclude the further testing of SCKs in an in vivo mouse immunization protocol. In this limited assay, measured increases in immunoglobulin G (IgG) concentration in the sera were minimal with no specific interactions being isolated, and more importantly, none of the mice (>50) subjected to the three 100 microg immunization protocol have died. Additionally, no gross morphological changes were observed in postmortem organ histology examinations.