gebhart/patrepeval huggingface dataset:
Magnetic properties of multilayered amorphous magnetic films (1980)

The magnetic properties of multilayered films consisting of an amorphous Gd-Co layer having a uniaxial magnetic anisotropy perpendicular to the film plane, and a Permalloy or a Tb-Fe layer having an in-plane anisotropy are studied. The thickness dependence of the shift of compensation temperature is studied in the amorphous Gd-Co film coated with Permalloy. When the Permalloy film is thicker than 600Å, the shift of compensation temperature becomes constant (about 5°C). Above a thickness of 2500Å of the amorphous Gd-Co film, the shift of compensation temperature becomes constant ( about 5°C). On the other hand, if the amorphous Gd-Co film is 1500Å thick, the change of compensation temperature does not occur. In the multilayered film having a nonmagnetic layer between the amorphous Gd-Co and the Permalloy film, no shift of compensation temperature occurs. The magnetic properties of thin Tb-Fe films coated on amorphous Gd-Co films have also been studied. When the thickness of the Tb-Fe is greater than about 100Å and thinner than about 500Å, the polarity of the Kerr hysteresis loops in double layered films changes.
High-Performance Field Emitters of Multiwalled Carbon Nanotubes Grown by Varying Oxygen Gas Pressure Using Arc Discharge (2018)

High-current field emission from carbon nanotube (CNT) emitters usually force them to be exposed to high electrostatic forces and Joule heating. Vacuum packaging of field emission devices is also often performed at high temperature. Under these circumstances, CNT emitters may be easily damaged and thus require highly crystalline and thermally stable CNTs. In this study, CNT paste was prepared using highly crystalline CNTs grown arc discharge. This study optimized oxygen gas pressure for the arc discharge growth of CNTs in terms of their crystallinity and thermal stability. Compared with the CNTs grown by catalytic chemical vapor deposition, the arc-produced CNTs showed better lifetime performance, probably due to their higher crystallinity.
Regulation of Kiss1 Gene Expression in the Brain of the Female Mouse (2005)

The Kiss1 gene encodes a family of neuropeptides called kisspeptins, which activate the receptor G protein-coupled receptor-54 and play a role in the neuroendocrine regulation of GnRH secretion. We examined whether estradiol (E2) regulates KiSS-1 in the forebrain of the female mouse by comparing KiSS-1 mRNA expression among groups of ovary-intact (diestrus), ovariectomized (OVX), and OVX plus E2-treated mice. In the arcuate nucleus (Arc), KiSS-1 expression increased after ovariectomy and decreased with E2 treatment. Conversely, in the anteroventral periventricular nucleus (AVPV), KiSS-1 expression was reduced after ovariectomy and increased with E2 treatment. To determine whether the effects of E2 on KiSS-1 are mediated through estrogen receptor (ER)alpha or ERbeta, we evaluated the effects of E2 in OVX mice that lacked functional ERalpha or ERbeta. In OVX mice that lacked functional ERalpha, KiSS-1 mRNA did not respond to E2 in either the Arc or AVPV, suggesting that ERalpha is essential for mediating the inhibitory and stimulatory effects of E2. In contrast, KiSS-1 mRNA in OVX mice that lacked functional ERbeta responded to E2 exactly as wild-type animals. Double-label in situ hybridization revealed that virtually all KiSS-1-expressing neurons in the Arc and AVPV coexpress ERalpha, suggesting that the effects of E2 are mediated directly through KiSS-1 neurons. We conclude that KiSS-1 neurons in the Arc, which are inhibited by E2, may play a role in the negative feedback regulation of GnRH secretion, whereas KiSS-1 neurons in the AVPV, which are stimulated by E2, may participate in the positive feedback regulation of GnRH secretion.
Unidirectional, heterologous desensitization of the pertussis toxin receptor by the CD3/TCR complex (1992)

Prolonged exposure of many types of receptors to their cognate agonists can lead to a progressive lack of responsiveness. When this occurs after stimulation by the primary agonist for a given receptor it is termed homologous desensitization, and heterologous desensitization when to an agonist binding to a different type of receptor. Pertussis toxin (PTx) is a potent mitogen for human T lymphocytes. We have previously identified the human T cell PTx receptor (PTx-R) as a 43-kDa plasma membrane protein that, when stimulated, leads to the production of the intracellular second messengers, inositol-1,4,5-triphosphate, 1,2-sn-diacylglycerol, and elevated cytosolic calcium. The PTx-R appears to require the co-expression of the CD3/TCR complex because mutant cells that lack the AgR, but express the PTx-R, fail to respond to PTx. In this report, we have investigated the relationship between these two receptor systems. Activation of the PTx-R with submaximal concentrations of PTx did not affect the ability of an anti-CD3 antibody combined with rabbit anti-mIg to stimulate increases in intracellular free calcium concentration [Ca2+]i or diacylglycerol in human peripheral blood T cells. However, treatment with soluble anti-CD3 mAb, which lead to only a modest increase in [Ca2+]i, completely inhibited the effect of PTx. The cells were not refractory to further stimulation of the AgR because cross-linking with rabbit anti-mIg resulted in the standard maximal stimulation. This effect could be observed within 1 min of treatment with anti-CD3 mAb, and persisted for at least 1 h. The effect was not caused by production of either diacylglycerol (leading to activation of PK-C) or an increase in [Ca2+]i by anti-CD3 mAb because the effect could not be mimicked by either phorbol esters or a calcium ionophore. Pretreatment of either resting T lymphoblasts or PBL with anti-CD3 mAb also prevented enhanced [3H]TdR incorporation stimulated by PTx. These observations suggest a model in which T cells can regulate amplification of a non-AgR stimulatory pathway by heterologous desensitization.
The Poincare sphere: isolated and real trajectories describing the state of polarization (1992)

For a given crystal optics device with polarization properties there are two kinds of trajectories that can be drawn on the Poincare sphere, namely the isolated and real trajectories. Using Stokes vectors and Mueller matrices, the parametric equations that represent the evolution of the state of polarization due to the passage of light through polarizers and optical components exhibiting linear or circular birefringence are given. The geometrical trajectories given by these equations, are useful for analyzing or designing optical birefringent networks and they are easy to obtain with today's computer software. An example of their application in the analysis of a Solc filter is given.
<i>In vitro</i> Plant Formation from Stem Explants of Rape (<i>Brassica napus</i> cv. Zephyr) (1974)

Abstract Internodal segments from 6‐weeks‐old rape plants ( Brassica napus L. cv. Zephyr) were induced to differentiate in vitro producing shoots or shoots and roots on synthetic nutrient medium under controlled conditions. Benzyladenine (BA) alone (5 × 10 −6 M) induced multiple shoot formation on all stem explants. Roots were induced on shoots when recultured on nutrient medium supplemented with auxins such as naphthalene‐acetic acid (NAA) or indoleacetic acid (1AA) or when planted in vermiculite. Complete plant formation was obtained when NAA (2 × 1 −6 , 5 × 10 −6 and 10 −5 M) was employed in conjunction with BA at 5 × 10 −6 M. At higher concentrations (10 −5 M ) NAA retards the shoot development while 1AA suppresses it totally. Lower levels of auxins along with the cytokinin did not retard or inhibit shoot differentiation.
Synthesis of α-Acetyl Lactones; Access to 14-Membered Bislactones by Attempts at Direct Ring Closure (2002)

α-Acetyl lactones are accessed by Mukaiyama-Claisen reactions. Attempts at direct 7-membered ring closure, by either intramolecular alkylation of 4-iodobutyl acetoacetate or olefin meta­thesis from allyl 2-allyl-3-oxobutanoate, afford exclusively dimeric 14-membered ring products instead of the expected caprolactones.
Synthesis, Stabilization, and Electronic Structure of Quantum Semiconductor Nanoclusters (1989)

This article reviews published data on the mechanical properties of additively manufactured metallic materials. The additive manufacturing techniques utilized to generate samples covered in this review include powder bed fusion (e.g., EBM, SLM, DMLS) and ...Read More
Integrated multi media and visualisation techniques for process S&C (2002)

In this contribution it will be shown how new forms of process visualisation and the integration of different information media can greatly help the operators of complex plants in supervisory control (S&C). The process visualisation aspects encompass process overview as well as detailed and context-specific yet interactive presentation of process information. The multimedia aspects show how the well-designed integration of video in particular can lead to an 'augmented reality' that helps to narrow the gap between central control rooms and the actual plant. An example from the application domain of power plant control illustrates the benefits.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Detection of Lawsonia intracellularis by Real-time PCR in the Feces of Free-living Animals from Equine Farms with Documented Occurrence of Equine Proliferative Enteropathy (2008)

The objective of this study was to determine whether Lawsonia intracellularis was present in the feces of free-living animals collected on two equine premises with documented occurrence of equine proliferative enteropathy (EPE). Fresh feces from black-tailed jackrabbits (Lepus californicus, n=100), striped skunks (Mephitis mephitis, n=22), feral cats (Felis catus, n=14), Brewer's Blackbirds (Euphagus cyanocephalus, n=10), Virginian opossums (Didelphis virginiana, n=9), raccoons (Procyon lotor, n=4), California ground squirrels (Spermophilus beecheyi, n=3), and coyotes (Canis latrans, n=2) were collected from August 2006 to January 2007 either from the ground while walking the premises or after trapping the animals using live traps. Nucleic acid purified from feces was directly processed for polymerase chain reaction (PCR) analysis using a real-time PCR assay targeting the aspartate ammonia lyase gene of L. intracellularis. Purified DNA samples were also precipitated, preamplified for L. intracellularis, and analyzed using the same real-time PCR assay, to increase the detection limit to one L. intracellularis organism per extracted sample. Feces from jackrabbits, striped skunks, Virginian opossums, and coyotes tested PCR positive for L. intracellularis, whereas all feces from feral cats, Brewer's Blackbirds, raccoons, and ground squirrels tested PCR negative for L. intracellularis. PCR testing on DNA extracted directly from feces was positive for L. intracellularis in six of 164 fecal samples. When DNA purification from feces was followed by a precipitation and preamplification step, five additional fecal samples tested PCR positive for L. intracellularis (11/164). The largest number of PCR positive L. intracellularis fecal samples was observed in striped skunks, followed by Virginian opossums, jackrabbits, and coyotes. This is the first description of L. intracellularis in these four species. Because the fecal samples were collected at equine farms with confirmed cases of EPE, striped skunks, Virginian opossums, jackrabbits, and coyotes may act as potential sources of infection to susceptible weanlings.
Aminoglycosides: Molecular Insights on the Recognition of RNA and Aminoglycoside Mimics (2009)

RNA is increasingly recognized for its significant functions in biological systems and has recently become an important molecular target for therapeutics development. Aminoglycosides, a large class of clinically significant antibiotics, exert their biological functions by binding to prokaryotic ribosomal RNA (rRNA) and interfering with protein translation, resulting in bacterial cell death. They are also known to bind to viral mRNAs such as HIV-1 RRE and TAR. Consequently, aminoglycosides are accepted as the single most important model in understanding the principles that govern small molecule-RNA recognition, which is essential for the development of novel antibacterial, antiviral or even anti-oncogenic agents. This review outlines the chemical structures and mechanisms of molecular recognition and antibacterial activity of aminoglycosides and various aminoglycoside mimics that have recently been devised to improve biological efficacy, binding affinity and selectivity, or to circumvent bacterial resistance.
In Vitro Study of Inflammatory Potential and Toxicity Profile of Latanoprost, Travoprost, and Bimatoprost in Conjunctiva-Derived Epithelial Cells (2005)

The comparison of latanoprost, travoprost, and bimatoprost, in their commercial formulations, showed that none of them appeared to induce direct stimulation of the inflammatory pathways involving adhesion molecules or class II antigens, although these markers have been found ex vivo in conjunctival specimens from patients treated with prostaglandins. In fact, their toxicity was mild and seemed to be primarily related to the concentration of BAC, their common preservative, which may be the major factor responsible for long-term ocular surface reactions in patients receiving topical prostaglandins, but most likely is not a factor in early and transient conjunctival hyperemia.
Physics-based approaches to modeling surface color perception (1999)

The diversity of color results from the diversity of surfaces which absorb the light – Ulrich von Strassburg (1262)
Robust and Flexible Films from 100% Starch Cross-Linked by Biobased Disaccharide Derivative (2015)

In this research, oxidized sucrose, a novel aldehyde-based green cross-linker, endowed starch films with substantial improvement in both tensile strength and elongation, whereas many other cross-linkers did not. Starch films are usually weak, brittle, and highly moisture sensitive, and thus have restricted industrial applications. Cross-linking is one of the most common methods to tackle these problems. However, most of the available cross-linkers are either toxic, expensive, or with low cross-linking efficiencies. Oxidized sucrose is a green cross-linker with multiple aldehyde groups per molecule to cross-link starch molecules via forming hemiacetals/acetals. The starch films cross-linked with oxidized sucrose had tensile strength and breaking elongation of 23 MPa and 60%, respectively, exceeding the cross-linking results of many other cross-linkers. Oxidized sucrose cross-linking also substantially increased the stability of starch films in both water and formic acid. With activation energy as low as 33.22 kJ mol–1, the cross-linking, a pseudo-first-order reaction, could occur readily. Mild cross-linking using oxidized sucrose might provide an alternative to promote industrialization of starch-based products.
Ultra flat high resolution microscanners (2007)

We present a high frequency (30 kHz) micro-scanner with 27 nm dynamic deformation at plusmn10deg mechanical scan angle. To achieve that, a dry-wet combination process is utilized to fabricate reinforcement frames for scanner flatness improvement.
Influence of surface texture and charge on the biocompatibility of endovascular stents. (1995)

Stent surface texture was the most important factor determining biocompatibility of coated Palmaz-Schatz stents in this study. In contrast to suggestions derived from in-vitro studies, the charge of stents does not seem to play a major role with respect to stent thrombogenicity. Low stent charge correlates with an increased neointima formation.
Analysis and modeling of world wide web traffic (1998)

This dissertation deals with monitoring, collecting, analyzing, and modeling of World Wide Web (WWW) traffic and client interactions. The rapid growth of WWW usage has not been accompanied by an overall understanding of models of information resources and their deployment strategies. Consequently, the current Web architecture often faces performance and reliability problems. Scalability, latency, bandwidth, and disconnected operations are some of the important issues that should be considered when attempting to adjust for the growth in Web usage. The WWW Consortium launched an effort to design a new protocol that will be able to support future demands. Before doing that, however, we need to characterize current users' interactions with the WWW and understand how it is being used. We focus on proxies since they provide a good medium or caching, filtering information, payment methods, and copyright management. We collected proxy data from our environment over a period of more than two years. We also collected data from other sources such as schools, information service providers, and commercial aites. Sampling times range from days to years. We analyzed the collected data looking for important characteristics that can help in designing a better HTTP protocol. We developed a modeling approach that considers Web traffic characteristics such as self-similarity and long-range dependency. We developed an algorithm to characterize users' sessions. Finally we developed a high-level Web traffic model suitable for sensitivity analysis. As a result of this work we develop statistical models of parameters such as arrival times, file sizes, file types, and locality of reference. We describe an approach to model long-range and dependent Web traffic and we characterize activities of users accessing a digital library courseware server or Web search tools. Temporal and spatial locality of reference within examined user communities is high, so caching can be an effective tool to help reduce network traffic and to help solve the scalability problem. We recommend utilizing our findings to promote a smart distribution or push model to cache documents when there is likelihood of repeat accesses.
Immune Profile of Infectious Bursal Disease (IBD). II. Effect of IBD Virus on Pokeweed-Mitogen-Stimulated Peripheral Blood Lymphocytes of Chickens (1980)

Pokeweed-mitogen-stimulated peripheral blood lymphocytes from chickens infected with infectious bursal disease virus (IBDV) at 1 day and 3 weeks of age, together with those from uninfected age-matched control chickens, were examined at weekly intervals for their capacity to undergo in vitro terminal differentiation. This study included the determination of cytoplasmic-immunoglobulin (CIg)-containing peripheral blood lymphocytes and those bearing the surface membrane immunoglobulin (SmIg) cells to detect any defect in immunoglobulin synthesis and secretion, respectively. The results of our study indicated that there was a highly significant and pronounced progressive depletion of CIg-containing cells in chickens infected at 1 day of age. These findings suggest that IBDV affected the "immature" or precursor B cells to a far greater extent than mature B lymphocytes.
The Docking Interaction of Caspase-9 with ERK2 Provides a Mechanism for the Selective Inhibitory Phosphorylation of Caspase-9 at Threonine 125 (2007)

Caspase-9 plays a critical role in the initiation of apoptosis by the mitochondrial pathway. Activation of caspase-9 is inhibited by phosphorylation at Thr(125) by ERK1/2 MAPKs in response to growth factors. Here, we show that phosphorylation of this site is specific for these classical MAPKs and is not strongly induced when JNK and p38alpha/beta MAPKs are activated by anisomycin. By deletion and mutagenic analysis, we identify domains in caspase-9 and ERK2 that mediate their interaction. Binding of ERK2 to caspase-9 and subsequent phosphorylation of caspase-9 requires a basic docking domain (D domain) in the N-terminal prodomain of the caspase. Mutational analysis of ERK2 reveals a (157)TTCD(160) motif required for recognition of caspase-9 that acts independently of the putative common docking domain. Molecular modeling supports the conclusion that Arg(10) in the D domain of caspase-9 interacts with Asp(160) in the TTCD motif of ERK2. Differences in the TTCD motif in other MAPK family members could account for the selective recognition of caspase-9 by ERK1/2. This selectivity may be important for the antiapoptotic role of classical MAPKs in contrast to the proapoptotic roles of stress-activated MAPKs.
Induction of tumor‐cell lysis by bi‐specific antibody recognizing ganglioside GD2 and T‐cell antigen CD3 (1993)

Human tumor cells expressing ganglioside GD2 were lysed by various effector populations targeted with an anti-CD3-anti-GD2 bi-specific antibody (BAb CD3 x GD2). This antibody-heteroconjugate was prepared by chemically cross-linking the OKT-3 monoclonal antibody (MAb) reactive with CD3 antigen on T lymphocytes with the ganglioside MAb ME 361, which binds preferentially to the tumor-associated ganglioside GD2. The specificity of target-cell lysis by the cytotoxic T cells (CTL) was mediated by the specificity of the targeting antibody: GD2-negative cells were not lysed in the presence of the CD3 x GD2 BAb. A dose-dependent response was observed in a range of 10 to 10,000 ng/ml. In contrast, 2 other BAbs recognizing the tumor-associated antigens EGF-R and TKB-2 had greater potency to mediate tumor-cell lysis than the GD2 x CD3 BAb. Peripheral-blood cells (PBL) stimulated with OKT-3 MAb or with irradiated tumor cells in a mixed lymphocyte culture (MLTC) could be induced to lyse GD2-positive tumor cells in the presence of CD3 x GD2 BAb. The tumor-cell lysis could be mediated by autologous or allogeneic effector cells. NK cells had no influence on the BAb-induced cytotoxicity.
Gene-directed mutagenesis in bacteriophage T7 provided by polyalkylating RNAs complementary to selected DNA sites. (1980)

Bacteriophage T7 early transcripts were used as carriers of alkylating groups to affect complementary T7 DNA sites for inducing mutations in preselected genes. A heterofunctional polyalkylating agent N,N,N'-tri-(beta-chloroethyl)-N'-(p-formylphenyl)propylene diamine-1,3 was attached to 3-5% of the transcript nucleotides. The controlled alkylating groups carried on RNA were activated after RNA . DNA hybridization. The modified transcripts were shown to hybridize only with the complementary H strand and to form covalently bound R loops in the appropriate T7 sites. The T7 DNA molecules locally alkylated by the modified transcripts of gene 1.3 coding for T7 ligase were packaged into T7 proteins and used to infect Escherichia coli B. As judged by plating efficiency and the ligase-deficient E. coli BL2 strain, 4 of 140 plaques obtained after infection contained mutants defective in gene 1.3 in amounts of 0.7-1.5%. The T7 DNA locally alkylated by the modified transcripts of gene 0.3 and 1.1 were used for the transfection of E. coli C1757. Analysis of 24 plaques produced by transfection indicated that 3 contained mutants defective in gene 0.3 in amounts of 2-10%. The mutants had also a second unidentified mutation. Complementation analysis data suggest that the second mutation is due to a defect in gene 1.1. The results obtained demonstrate the efficiency of the approach developed for gene-directed mutagenesis.