gebhart/patrepeval huggingface dataset:
Enhancement of Infectivity of a Non‐Syncytium Inducing HIV‐1 by sCD4 and by Human Antibodies that Neutralize Syncytium Inducing HIV‐1 (1995)

Enhancement of virus infectivity after sCD4 treatment has been documented for SIVagm and HIV-2. It has been suggested that a similar phenomenon may play a role in HIV-1 infection. In the present study we have analysed biological activities of virus neutralizing polyclonal and monoclonal human antibodies and of sCD4, towards HIV-1 chimeras with envelope proteins derived from one donor, which display different biological phenotypes. The antibodies, which recognize the V3 and/or the CD4 binding domains of the glycoproteins of these viruses and also sCD4 showed different levels of virus neutralizing activity toward the syncytium inducing HIV-1 strains. In contrast, they all dramatically enhanced the infectivity of an HIV-1 chimera with an envelope glycoprotein displaying the non-syncytium-inducing phenotype. Given the relatively conserved nature of non-syncytium-inducing HIV-1 surface glycoproteins early after infection, these data suggest a major role for antibody mediated enhancement of virus infectivity in the early pathogenesis of HIV-1 infection.
The path towards resource elasticity for 5G network architecture (2018)

Vertical markets and industries are addressing a large diversity of heterogeneous services, use cases, and applications in 5G. It is currently common understanding that for networks to be able to satisfy those needs, a flexible, adaptable, and programmable architecture based on network slicing is required. Moreover, a softwarization and cloudification of the communications networks is already happening, where network functions (NFs) are transformed from monolithic pieces of equipment to programs running over a shared pool of computational and communication resources. However, this novel architecture paradigm requires new solutions to exploit its inherent flexibility. In this paper, we introduce the concept of resource elasticity as a key means to make an efficient use of the computational resources in 5G systems. Besides establishing a definition as well as a set of requirements and key performance indicators (KPIs), we propose mechanisms for the exploitation of elasticity in three different dimensions, namely computational elasticity in the design and scaling of NFs, orchestration-driven elasticity by flexible placement of NFs, and slice-aware elasticity via cross-slice resource provisioning mechanisms. Finally, we provide a succinct analysis of the architectural components that need to be enhanced to incorporate elasticity principles.
Amine dehydrogenase of Pseudomonas putida: properties of the heme-prosthetic group (1978)

There was approximately five times more hemoprotein (amine dehydrogenase) in crude extracts obtained from Pseudomonas putida grown on benzylamine than present in extracts from succinate-grown cells. The difference (reduced minus oxidized) spectrum of the purified enzyme possessed alpha,beta, and gamma bands at 550, 523, and 416 nm, respectively. The difference spectrum of the pyridine hemochrome derivative had absorption maxima at 416, 520, and 550 nm. These results, together with the fact that the heme group was covalently bound to the enzyme, indicated that the amine dehydrogenase from P. putida was a hemoprotein which contained heme c. The heme content was calculated at 2.01 mol/mol of enzyme. The enzyme was composed of two nonidentical subunits, but heme was present solely in the heavier unit. Carbon monoxide did not inhibit enzymatic activity, nor would it combine with the reduced or oxidized form of the enzyme. Amine dehydrogenase activity was inhibited by carbonyl agents with semicarbazide and cuprizone acting noncompetitively, whereas KCN and isoniazid inhibited by competitive and uncompetitive mechanisms, respectively. Spectral observations suggested that inhibition by these reagents was not due to an interaction with the heme moiety.
Voice Conversion Using Deep Neural Networks With Layer-Wise Generative Training (2014)

This paper presents a new spectral envelope conversion method using deep neural networks (DNNs). The conventional joint density Gaussian mixture model (JDGMM) based spectral conversion methods perform stably and effectively. However, the speech generated by these methods suffer severe quality degradation due to the following two factors: 1) inadequacy of JDGMM in modeling the distribution of spectral features as well as the non-linear mapping relationship between the source and target speakers, 2) spectral detail loss caused by the use of high-level spectral features such as mel-cepstra. Previously, we have proposed to use the mixture of restricted Boltzmann machines (MoRBM) and the mixture of Gaussian bidirectional associative memories (MoGBAM) to cope with these problems. In this paper, we propose to use a DNN to construct a global non-linear mapping relationship between the spectral envelopes of two speakers. The proposed DNN is generatively trained by cascading two RBMs, which model the distributions of spectral envelopes of source and target speakers respectively, using a Bernoulli BAM (BBAM). Therefore, the proposed training method takes the advantage of the strong modeling ability of RBMs in modeling the distribution of spectral envelopes and the superiority of BAMs in deriving the conditional distributions for conversion. Careful comparisons and analysis among the proposed method and some conventional methods are presented in this paper. The subjective results show that the proposed method can significantly improve the performance in terms of both similarity and naturalness compared to conventional methods.
People and Planning: Report of the Committee on Public Participation in Planning (The Skeffington Committee Report) (2017)

Peter Shapely. London, UK, and New York, NY: Routledge, 2014. 71 pp. £100.00 cloth (ISBN 978-0415827874).People and Planning is a British government report originally released in 1969 to provide re...
Atomic Layer Deposition of Rhenium–Aluminum Oxide Thin Films and ReO<sub><i>x</i></sub> Incorporation in a Metal–Organic Framework (2017)

Methyltrioxorhenium (ReO<sub>3</sub>Me) is introduced as the first rhenium atomic layer deposition (ALD) precursor and used to grow rhenium-aluminum oxide thin films in combination with trimethylaluminum (TMA-AlMe<sub>3</sub>). The growth rate of the smooth Re-Al oxide films, with general stoichiometry Re<sub>x</sub>Al<sub>y</sub>O<sub>3x</sub>, has been monitored by in situ quartz crystal microbalance (QCM) and ex situ ellipsometry, and found to be 3.2 Å/cycle. X-ray photoelectron spectroscopy (XPS) revealed the mixed valent composition of the film with Re(III) species being the main component. In addition, ReO<sub>3</sub>Me has been successfully used to deposit rhenium oxide in NU-1000, a mesoporous zirconium-based metal-organic framework (MOF). The metalated MOF was found to retain porosity and crystallinity and to be catalytically active for ethene hydrogenation.
Lasing without inversion (2000)

This review paper is devoted to amplification and lasing without population inversion involving atomic transitions in gas media. We start by discussing the main motivation in inversionless lasing research, namely, the generation of short-wavelength laser light. Then, we review the basic physics of inversionless lasing in two-level and, eventually, in three- and multilevel atomic configurations. Finally, we summarize the current state of the art of LWI experiments and indicate the main difficulties with respect to short-wavelength laser generation.
New Bilayer Positive Photoresist for 193 nm Photolithography (1999)

Abstract We have designed a function-integrated acrylic terpolymer, poly(MMAx-BPMAy-MAAz) which has both a dry-etching resistant unit (trimethylsilyl group) and a deprotecting group. A new terpolymer was synthesized by free radical terpolymerization of methyl methacrylate (MMA), 1,3-bis(trimethylsilyl)isopropyl methacrylate (BPMA) and meihacrylic acid (MAA). Thermogravimetric analysis (TGA) of terpolymer showed a good thermal stability up to 140°C. We achieved a minimum feature resolution with 0.17 μm L/S pattern in an ArF exposure system using poly(MMA5,9-BPMA2,1-MAA2,0) resist.
Betacellulin induces angiogenesis through activation of mitogen‐activated protein kinase and phosphatidylinositol 3’‐kinase in endothelial cells (2002)

Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family, and it acts through EGF receptors. We asked whether BTC could be an angiogenic factor. Using human umbilical vein endothelial cells (HUVECs), we examined the effect of BTC on kinases and angiogenic processes. BTC induced ERK1/2 and Akt phosphorylation in a dose- and time-dependent manner. BTC induced phosphorylation of all three EGF receptors present on HUVECs: ErbB2, ErbB3, and ErbB4. Pretreatment with effective concentrations of ErbB1 inhibitor did not suppress BTC-induced kinase phosphorylation. BTC, EGF, VEGF (all at 10 ng/ml) produced similar increases in DNA synthesis. BTC, EGF, and VEGF all significantly increased endothelial cell migration. In addition, BTC promoted survival in a dose-dependent manner, and its effect was inhibited by pretreatment with PtdIns 3'-kinase inhibitor wortmannin or MEK1/2 inhibitor PD98059. Both BTC and EGF produced similar increases in tube formation in collagen gels. BTC-induced tube formation was suppressed by PD98059, wortmannin, and LY294002. In the mouse Matrigel plug assay, BTC (100 ng/ml) promoted neovessel formation, and its effect was suppressed by a combination of wortmannin and PD98059. Taken together, these data show that BTC exerts potent angiogenic activity through activation of EGF receptors, mitogen-activated protein kinase, and PtdIns 3'-kinase/Akt in endothelial cells.
Outcomes after detection of metastatic carcinoma of the colon and rectum in a national hospital system. (1996)

Resection of isolated colorectal metastases produced 70 five-year survivors (hepatic, n=42, pulmonary, n=28) and accounted for 446 additional years of patient life over that expected without resection.
Design of Poly(vinyldiaminotriazine)‐Based Nonviral Vectors via Specific Hydrogen Bonding with Nucleic Acid Base Pairs (2006)

Abstract A novel gene transfer system that complexes plasmid DNA (pDNA) through complementary hydrogen bonding of guests with base pairs is reported. Poly(vinyldiaminotriazine) (PVDT) homopolymer and its copolymers with poly(1‐vinyl‐2‐pyrrolidone) are synthesized via conventional radical random polymerization, and water‐soluble fractions are collected. These PVDT‐based polymers efficiently complex pDNA and displace ethidium bromide (EB) in EB‐intercalated pDNA solutions because of the hydrogen‐bonding‐induced constrained state of pDNA. It is also found that upon complexation, pDNA seems to undergo B–C conformation change and circular dichroism spectra assumed a polymer‐and‐salt‐induced (psi)‐type pattern that is rationally ascribed to a certain change in the high‐order structure of DNA condensates. Transmission electron microscopy presented several morphologies of spheres and toroids within aggregates ≥ 100 nm, resembling DNA condensation induced by cationics. In transfection studies using pDNA‐encoding luciferase or enhanced green fluorescent protein, this system could efficiently transfect COS‐1 cells. Compared to the commercial polycation system, ExGen 500, these H‐bonding vectors display several merits such as higher transfection efficiency, lower cytotoxicity, better serum compatibility, and stability in the presence of bovine serum albumin (BSA). The results suggest that PVDT‐based polymers are superior to polycation counterparts with regard to their potential in vivo applications.
Online access to knowledge: System design (1989)

The project Online Access to Knowledge (OAK) has developed a computer intermediary for selected users of the Department of Energy's DOE/RECON and BASIS online information retrieval systems. Its purpose is to enable people who have little or no training or experience in bibliographic searching to conduct their own searches, without the assistance of a trained librarian, hence permitting the user to work in both a place and time of his or her choosing. The purpose of this article is to report on the design and the rationale for the design. OAK software consists of both a tutorial and an assistance program. The latter does not employ a command language, hence obviates the need for a searcher to learn the formal language usually associated with an online database search service. It is central to our approach that this system does not supplant the user's ultimate primacy in knowing what he or she is looking for, nor in judging the results. © 1989 John Wiley & Sons, Inc.
Distinguishing the viability of a single yeast cell with an ultra-sensitive radio frequency sensor (2010)

We propose and demonstrate a simple, ultra sensitive radio frequency (RF) sensor to detect a single yeast cell and distinguish its viability in a microfluidic channel. On-chip interference is used to cancel background probing signals to improve sensor sensitivity. Individual viable and nonviable yeast cells (approximately 5.83 +/- 0.85 microm in diameter) are measured with clear sensing and identification of these cells.
Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp. nov., Acetobacter cibinongensis sp. nov., and Acetobacter orientalis sp. nov. (2001)

Forty-six strains of acetic acid bacteria newly isolated from flowers, fruits, and fermented foods collected in Indonesia were taxonomically studied. They were Gram-negative rods, produced acetic acid from ethanol, oxidized acetate and lactate to CO(2) and H(2)O, and had Q-9 as the major ubiquinone system. On the basis of DNA-DNA similarity, all strains studied, including type strains and reference strains of the genus Acetobacter, were separated into eleven groups (Groups I to XI). Of the 46 isolates, two isolates were included in Group II and identified as Acetobacter pasteurianus, five in Group IV as A. orleanensis, 16 in Group V as A. lovaniensis, five in Group VII as A. indonesiensis, and three in Group VIII as A. tropicalis. The remaining 15 isolates constituted three new groups based on DNA-DNA similarity; four isolates were included in Group IX, two in Group X, and nine in Group XI. No isolates were identified as A. aceti (Group I), A. peroxydans (Group III), and A. estunensis (Group VI). Phylogenetic analysis based on 16S rDNA sequences of representative strains of the Groups indicated belonging to the strains of the genus Acetobacter. On the basis of DNA base composition, DNA-DNA similarity, and 16S rDNA sequences, three new species of the genus Acetobacter are proposed: Acetobacter syzygii sp. nov. for Group IX, Acetobacter cibinongensis sp. nov. for Group X, and Acetobacter orientalis sp. nov. for Group XI. The distribution of Acetobacter strains in Indonesia is discussed in light of isolation sources.
Nonuniformly Controlled Analog-to-Digital Converter for SDR Multistandard Radio Receiver (2011)

A new multistandard radio receiver architecture is presented to take advantage of nonuniformly controlled analog-to-digital converters (ADCs). By defining the statistical parameter related to the random sampling frequency, a time-quantized random sampling scheme is considered to design a nonuniform sampling-based software defined radio (SDR) multistandard receiver. A relaxed design is proposed for an E-GSM/UMTS/IEEE802.11a multistandard receiver. The designed baseband stage avoids the use of the automatic gain control block, relaxes the nonprogrammable antialiasing filter to the third order, and converts data with a 16-bit ADC at a 124-MHz mean sampling frequency.
Reconstitution of cyclin-dependent cdc2 and cdk2 kinase activities in vitro. (1993)

The genes that encode human cdc2 and cdk2 proteins are essential for cell cycle progression. In this report, we describe the purification of cyclin-associated cdc2 and cdk2 kinases as well as cyclin-free cdc2 and cdk2 protein preparations from HeLa cells. The cdc2-cyclin B kinase complex that we have isolated, consisting of two polypeptides of p60 (cyclin B) and p34 (cdc2), phosphorylated both the p34 and p70 subunits of the three-subunit human single-stranded DNA-binding protein (also called RP-A), a DNA replication and repair factor. We also partially purified a histone H1 kinase activity that is associated with the cdk2 and cyclin A proteins. Purified human cyclins A and B1, overproduced in bacteria, complemented a cellular fraction enriched in cdc2 and cdk2 proteins to reconstitute histone H1 kinase activity. Using this complementation system, human cdc2 and cdk2 proteins were purified and separated from one another. Glycerol gradient analyses demonstrated that the purified cdk2 (p33) protein co-sedimented with a cyclin A-dependent H1 kinase activity. Thus, cdk2 and cyclin A proteins are components that assemble to yield a kinase complex that catalyzes the phosphorylation of histone H1.
Restoration of impaired natural killer cell activity of B‐chronic lymphocytic leukemia patients by recombinant interleukin‐2 (1987)

Natural killer (NK) function in the majority of B-cell chronic lymphocytic leukemia patients is markedly deficient. This study was undertaken to determine if the biological response modifier interleukin-2 (IL-2), which is a potent augmenter of normal individuals' NK activity, could augment the low NK activity in these patients. Peripheral blood lymphocytes (PBL), depleted of B-cells, from most B-CLL patients exhibited low natural killer activity against NK-sensitive K562 cells and against herpes simplex virus (HSV)-infected lymphoblastoid cell lines (LCL). Incubation of patients' B-cell-depleted PBL with recombinant IL-2 resulted in augmentation of their NK activity against both K562 cells and HSV-infected cells. Furthermore, whereas large granular lymphocytes (LGL) isolated from CLL patients are deficient in cytoplasmic granules, which are thought to play a role in NK-cell-mediated lysis, treatment of patients' LGL resulted in increased granulation by 4 hr after treatment with IL-2 and showed a concomitant increase in lytic activity comparable to that of normal individuals.
The Brain of the Common Marmoset (Callithrix Jacchus): A Stereotaxic Atlas (1980)

Nanomaterials.- Fundamentals of Mechanics of Materials.- Nanoscale Mechanics and Materials: Experimental Techniques.- Mechanical Properties: Density and Elasticity.- Plastic Deformation of Nanomaterials.- Mechanical Failure Processes in Nanomaterials.- Scale-Dominant Mechanisms in Nanomaterials.- Modeling Nanomaterials.
Detection of human immunodeficiency virus type 1 clinical isolates with reduced sensitivity to zidovudine and dideoxyinosine by RNA.RNA hybridization. (1991)

A quantitative rapid assay to detect resistant clinical human immunodeficiency virus type 1 (HIV-1) strains remains an important medical goal. A system incorporating a quantitative RNA.RNA hybridization assay that measures the amount of intracellular HIV-1-specific RNA has been employed to detect the level of inhibition by nucleoside analogues in sensitive and resistant HIV-1 strains. The RNA.RNA hybridization assay readily distinguished previously published zidovudine (ZDV; 3'-azido-3'-deoxythymidine)-resistant isolates from ZDV-sensitive isolates of HIV-1. The 50% inhibitory concentration (IC50) of ZDV for HTLV-IIIB and sensitive clinical HIV-1 isolates is between 0.01 and 0.04 microM. HIV-1 strains from three patients on long-term ZDV therapy displayed a greater than 20-fold increase in the ZDV IC50 compared to sensitive strains. The drug sensitivity system was confirmed by showing that mutations in the HIV reverse transcriptase gene from a ZDV-resistant isolate resulted in four amino acid changes (Leu-125----Trp, Ile-142----Val, Thr-215----Tyr, and Pro-294----Thr) including one change (Thr-215----Tyr) that has been previously reported to be associated with resistance. One clinical HIV strain with high-level ZDV resistance displayed a 5-fold increase in 2',3'-dideoxyinosine IC50 compared to that of HTLV-IIIB. A drug sensitivity assay employing RNA.RNA hybridization may be useful for extensive screening of HIV isolates from patients enrolled in clinical trials and permit the correlation of in vitro resistance with clinical outcome.
Micromechanical gyroscope finite-element analysis (2008)

Mechanical analysis by method of finite-element analysis of the construction of two-component micromechanical gyroscope is explored. 3D geometric model and finite-element model of the gyroscope are made. Natural frequencies and mode shapes of the gyroscope are determined. Parameters ensuring resonance tuning are realized. The choice of the most appropriate variant of construction takes place by realizing of modal analysis and varing of sensor's geometry. Deformations of device construction elements determined at maximum of the measured velocity.
Deoxidization of water desorbed from APCVD TEOS-O/sub 3/ SiO/sub 2/ by titanium cap layer (1995)

The water absorption and desorption characteristics of APCVD TEOS-O/sub 3/ SiO/sub 2/ have been studied by exposing SiO/sub 2/ films deposited at 380/spl deg/C in different O/sub 3/ concentrations to a water-vapor ambient. The total amount of water desorbed from the film decreased with the increasing O/sub 3/ concentration. In contrast, water desorbed from the film at temperature lower than 300/spl deg/C increased. In the application of TEOS-O/sub 3/ SiO/sub 2/ as an intermetal dielectric, water desorption at lower temperatures causes the bursting of via holes during high-temperature storage tests. It was found that, however, a thin titanium film deoxidizes water desorbed from TEOS-O/sub 3/ SiO/sub 2/ to hydrogen. The bursting of via holes was completely suppressed by using thin titanium film between the aluminum and TEOS-O/sub 3/ SiO/sub 2/ because hydrogen passes more easily than water through the aluminum interconnect.
Fate of potassium 3,4-dichloro-5-isothiazolecarboxylate in cotton plants and white rats (1979)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFate of potassium 3,4-dichloro-5-isothiazolecarboxylate in cotton plants and white ratsTed N. Shaver, Don L. Bull, and G. Wayne IvieCite this: J. Agric. Food Chem. 1979, 27, 2, 325–328Publication Date (Print):March 1, 1979Publication History Published online1 May 2002Published inissue 1 March 1979https://pubs.acs.org/doi/10.1021/jf60222a004https://doi.org/10.1021/jf60222a004research-articleACS PublicationsRequest reuse permissionsArticle Views44Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts