gebhart/patrepeval huggingface dataset:
Pentosan polysulfate‐induced thrombocytopenia and thrombosis (1994)

Pentosan polysulfate is a low-molecular-weight sulfated polysaccharide used as an antithrombotic drug. We present two patients who developed thrombocytopenia and venous thrombosis during treatment with pentosan polysulfate. The relationship between pentosan polysulfate and thrombocytopenia is supported by platelet aggregation and serotonin release tests. In the light of the literature and our two cases, it appears that pentosan polysulfate alone as standard heparin and low-molecular-weight heparin can induce thrombocytopenia and thrombosis. Platelet counts should therefore be periodically monitored during pentosan polysulfate treatment. In the case of pentosan polysulfate-induced thrombocytopenia, it seems that heparin or low-molecular-weight heparin should not be instituted during the acute phase even if platelet aggregation studies are negative, because of their low sensitivity. After remission of thrombocytopenia, whether or not glycosaminoglycans can be reinstituted, at least temporarily, after antibody had disappeared is still an open question.
Initial steps of supercontinuum generation in photonic crystal fibers (2003)

The onset of supercontinuum generation in a photonic crystal fiber is investigated experimentally and numerically as a function of pump wavelength and intensity with 100-fs pulses. Soliton formation is found to be the determining factor in the initial step. The formation and behavior of a blueshifted, nonsolitonic component, emitted as the soliton evolves towards the stable regime, is investigated and the role of phase matching through higher-order dispersion is highlighted. Good agreement between experiments and simulations is obtained.
Critical parameters in the pegylation of gold nanoshells for biomedical applications: An<i>in vitro</i>macrophage study (2008)

Pegylation of gold nanoshells provides an effective means to reduce their reticuloendothelial system (RES) clearance in body. In this study, we perform a parametric investigation on the factors that would affect the macrophage uptake of gold nanoshells with the aim to optimize their pegylation and minimize their macrophage uptake. We synthesized and pegylated the gold nanoshells using methoxy-poly(ethylene glycol)-thiol and employed an in vitro macrophage assay to examine the effect of surface density of poly(ethylene glycol) (PEG), chain length of the PEG, and size of the gold nanoshells on their macrophage uptake. We have shown that a saturated surface density would minimize macrophage uptake, which could be obtained by experimental titration-based Ellman's reagent. Our results suggest that the chain length of PEG and size of gold nanoshells influence the surface density of PEG. We have also shown that PEG with molecular weight of around 2000Da and a size range larger than 186nm would be appropriate for facilitating a high surface density. Our in vitro macrophage system thus provides a good model to accurately predict the RES response to different pegylation parameters.
Real-time evaluation of tissue vitality by monitoring of microcirculatory blood flow, HbO 2 , and mitochondrial NADH redox state (2004)

Monitoring of tissue vitality (oxygen supply/demand) in real time is very rare in clinical practice although its use as an early warning alarming system, for clinical care medicine, is very practical. In our previous communication (SPIE 2003) we described the Tissue Spectroscope - TiSpec02, by which tissue microcirculatory blood flow (TBF) and mitochondrial NADH fluorescence were measured using a single light source (390nm). In order to improve the measurement capabilities as well as to decrease dramatically the size and cost of this clinical device, we have changed the TiSpec02 into a multi-wavelength illumination system in the new TiSpec03. In order to measure microcirculatory blood flow by laser Doppler flowmetry we used a 785nm laser diode. For mitochondrial NADH fluorescence measurement we adopted the 370nm LED. For the determination of the oxygenation level of hemoglobin (HbO<sub>2</sub>) we used the 2-wavelength reflectance technique. This new monitored parameter that was added to the TiSpec03 increases the accuracy of the diagnosis of tissue vitality. The bundle of optical fibers used to connect the tissue to the TiSpec03, was integrated into a special anchoring methodology depending on the monitored tissue or organ. In order to test the performance of the improved TiSpec we have used it in experimental animals brain models exposed to various pathophysiological conditions. Rats and gerbils were anesthetized and the fiber optic probe was located epidurally used dental acrylic cement. During anoxia and ischemia the lack of O<sub>2</sub> led to a clear decrease in TBF and HbO<sub>2</sub> while NADH shows a large elevation. When brain activation was induced by cortical spreading depression (SD), the elevated O<sub>2</sub> consumption was recorded as a large oxidation (decrease) of mitochondrial NADH while TBF increase dramatically. Blood HbO<sub>2</sub> was not affected significantly by the SD wave.
Different network coding techniques for multihop Machine-to-Machine (M2M) Communication in next generation smart environment (2017)

A typical intelligent factory, a smart home or a smart city consists of several nodes or machines. In a typical Machine to Machine Communication mode (M2M), each machine senses some information (pressure, temperature, presence of person, etc.) and then communicates this information to other machines. This communication could be unicast or multicast depending on the application. Further, the destination node of the M2M communication is many-a-times not in the direct communication range. In the presence of tens and hundreds of machines forming a network, it is essential to have an efficient communication mechanism for multihop multicast transmission between different machines. This paper proposes a network coding (NC) based multihop multicast communication for different topologies - butterfly, flattened butterfly, hypercube and folded-clos model. It is observed that random linear network coding (RLNC) technique outperforms best routing protocol for different topologies, by requiring less time for data transmission, reducing the time required by upto 50%, depending on the model. Further, in order to improve the performance of network coding for M2M in a disconnected network; an integrated network coding and routing approach is proposed and evaluated. It is observed that such an integrated mechanism requires upto 25% less time than pure routing and upto 10% less time than required by RLNC.
Theoretical study of physisorption of nucleobases on boron nitride nanotubes: a new class of hybrid nano-biomaterials (2010)

We investigate the adsorption of the nucleic acid bases—adenine (A), guanine (G), cytosine (C), thymine (T) and uracil (U)—on the outer wall of a high curvature semiconducting single-walled boron nitride nanotube (BNNT) by first-principles density functional theory calculations. The calculated binding energy shows the order: G > A ≈ C ≈ T ≈ U, implying that the interaction strength of the high curvature BNNT with the nucleobases, G being an exception, is nearly the same. A higher binding energy for the G–BNNT conjugate appears to result from hybridization of the molecular orbitals of G and the BNNT. A smaller energy gap predicted for the G–BNNT conjugate relative to that of the pristine BNNT may be useful in the application of this class of biofunctional materials to the design of next-generation sensing devices.
INTEGRATING USABILITY AND MARKETING ACTIVITIES (1991)

Although the practice of user-centered design is advocated in many organizations, engineering management issues may prevent early focus on system usability. Within the usability engineering cycle, practitioners consider task analysis and customer needs assessment a high priority. Market research activities occur early in the process, but usability information is not collected or passed to system developers. To improve and to accelerate the design process and to validate user preference findings, task analysis and market research surveys were performed concurrently during the design validation phase of a data communications product. The basic process steps included: forming a multidisciplinary team, identifying data sources, collecting data via interviews and event record review, transforming data into task models and product opinion matrices, and using the models and matrices to design a sample product. A number of benefits were observed using this method: improved economies of research and analysis time, increased strength of product opinion data, early product improvements, increased customer understanding of the product, and an improved product engineering process.
A murine model for the switch from fetal to adult hemoglobin during ontogeny (1980)

In murine erythroleukemia cells, the minor/major hemoglobin (Hb) ratio depends on the cell line and the inducing agent. To determine whether mouse minor hemoglobin is a “fetal” hemoglobin in vivo, globin chain composition and synthesis rates were determined in DBA/2 mice of various ages ranging from 14-day embryos to > 6-mo adults. Globin chains were separated by electrophoresis on polyacrylamide gels containing urea and Triton X-100. This method separates the embryonic (x,y,z) and the adult (alpha, beta ma, beta mi) globin chains. Fourteen day embryos had only 5%-10% adult globins but approximately 30% of the adult beta chains were beta mi. The % beta mi decreased with age and reached 20% in adult mice. Biosynthetic studies led to more pronounced differences: beta mi synthesis was 45% of total beta chain production in 14-day embryos and declined to 22% in adults. Thus beta minor/total beta globin synthesis declines during mouse ontogeny. This resembles qualitatively the human switch from fetal to adult hemoglobin and provides a murine model for studies of hemoglobin regulation.
Overview of DNA Sequencing Strategies (2011)

Abstract Efficient and cost‐effective DNA sequencing technologies are critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high‐level review of seven distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) solid phase sequencing; (c) sequencing‐by‐hybridization; (d) mass spectrometry; (e) cyclic array sequencing; (f) microelectrophoresis; and (g) nanopore sequencing. Other platforms currently in development are also briefly described. The primary focus here is on Sanger dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot as of September, 2011. Curr. Protoc. Mol. Biol . 96:7.1.1‐7.1.23. © 2011 by John Wiley & Sons, Inc.
Escherichia coli Glutamyl-tRNA Reductase (2002)

In the first step of tetrapyrrole biosynthesis in Escherichia coli, glutamyl-tRNA reductase (GluTR, encoded by hemA) catalyzes the NADPH-dependent reduction of glutamyl-tRNA to glutamate-1-semialdehyde. Soluble homodimeric E. coli GluTR was made by co-expressing the hemA gene and the chaperone genes dnaJK and grpE. During Mg(2+)-stimulated catalysis, the reactive sulfhydryl group of Cys-50 in the E. coli enzyme attacks the alpha-carbonyl group of the tRNA-bound glutamate. The resulting thioester intermediate was trapped and detected by autoradiography. In the presence of NADPH, the end product, glutamate-1-semialdehyde, is formed. In the absence of NADPH, E. coli GluTR exhibited substrate esterase activity. The in vitro synthesized unmodified glutamyl-tRNA was an acceptable substrate for E. coli GluTR. Eight 5-aminolevulinic acid auxotrophic E. coli hemA mutants were genetically selected, and the corresponding mutations were determined. Most of the recombinant purified mutant GluTR enzymes lacked detectable activity. Based on the Methanopyrus kandleri GluTR structure, the positions of the amino acid exchanges are close to the catalytic domain (G7D, E114K, R314C, S22L/S164F, G44C/S105N/A326T, G106N, S145F). Only GluTR G191D (affected in NADPH binding) revealed esterase but no reductase activity.
[Malignant B-cell lymphoma in a patient with primary Sjögren syndrome]. (1991)

Authors report the case of a 42 years old female with primary Sjögren's syndrome in whom nine years after the beginning of her disease generalised swollen lymphatic nodes and enlargement of liver as well as a spleen has appeared. The simultaneous appearance of fever, loss of body weight and worsening of her general condition raised the possibility of an associated malignant lymphoproliferate disease. The histology of a lymph node proved the presence of a highly malignant form of B-cell lymphoma (CB NHL). Attention is drawn to the necessitate of regular control of patients with Sjögren's syndrome. Lymphadenopathy, the decrease of the immunoglobulin levels and autoantibody titer in the serum, as well as the predominance of monoclonal immunoglobulins point to the possibility of malignant transformation. With reference to the presented case a short review of the Sjögren's syndrome is given by the authors.
Stability of bovine leukemia virus antigens. (1987)

Stability of bovine leukemia virus glycoprotein 51 and nonglycosylated protein 24 antigens was examined under various conditions. The glycoprotein antigen was unstable at 56 degrees C and 37 degrees C and at acidic and basic pH. The specificity of the glycoprotein 51 antigen was reduced to half by the first treatment with ethyl ether, but was not decreased further by repeated treatments. This antigen was not inactivated with triton X-100 and was resistant to lyophilization as well as to freezing and thawing. The protein 24 antigen was generally very stable under all conditions tested.
Topographic Control in the Toronto Region (1936)

The physical features controlling the economic development of a region can be classified under the two main headings of geological and climatic controls. All pastoral, agricultural, mining, or industrial life depends essentially on the distribution of minerals, soils, water supply, temperature, and rainfall. There is, however, another geological feature which exercises great influence on human interests, and that is the topography, i.e. , the arrangement of mountain and plain, of rivers and lakes, and all the elements which in more precise language build up the science of geomorphology. The present study attempts to show, first of all, what are the main features in southern Ontario, more particularly within a couple of hundred miles of Toronto, which have necessarily greatly affected the settlement of the region. Secondly the evolution of the city of Toronto as determined by the minor topographic features is described in somewhat general terms. It is hoped that this will pave the way to a more complete study of the city in the near future.
Cold trap/reinjection interface for two‐dimensional gas chromatography (1985)

Abstract The construction and evaluation of an interface for two‐dimensional gas chromatography is described. The interface consists of commercially available components and is attachable to available gas chromatographs without any major modifications. The interface has been constructed so as to permit “heartcutting” as well as solute band concentration. Trapping is performed in a simple cold trap of fused silica. Flow switching is accomplished by Deans switching. Factors of the over‐all chromatographic performance are examined.
PINK1-Parkin pathway (2015)

AccessScience is an authoritative and dynamic online resource that contains incisively written, high-quality educational material covering all major scientific disciplines. An acclaimed gateway to scientific knowledge, AccessScience is continually expanding the ways it can demonstrate and explain core, trustworthy scientific information that inspires and guides users to deeper knowledge.
Synovial detection and autoantibody reactivity of processed citrullinated isoforms of vimentin in inflammatory arthritides (2008)

These findings show the presence of processed citrullinated vimentin in inflammatory arthritides, mainly in RA and suggest a possible origin of the ACPA immune response in RA.
Characteristics and applications of selenium-rectifier cells (1943)

The rectification properties of selenium cells were first discovered in the year 1883. C. T. Fritts described them in the American Journal of Science. <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">11,12</sup> However, they were never used to any extent, except possibly as photocells. After the introduction of the copper-oxide rectifier, research activities were stimulated, and selenium cells were again rediscovered. The first commercial cells were made in Germany in the early 30's, and, as the technique of manufacture improved, better cells were made with better life expectancy. The General Electric Company, after a period of developmental activity, started to make cells in 1938, first in its research laboratories, and subsequently a manufacturing plant was set up which permits producing cells in large quantities within relatively close electrical tolerances. This paper contains data pertaining to these cells particularly, and the information may not apply in detail to cells manufactured by other methods without some correction factors.
Esophageal perforation due to nasogastric intubation. (1990)

Esophageal perforation is a rare but catastrophic complication of nasogastric intubation. Diagnosis is frequently delayed, resulting in high mortality. We report a case of perforation due to nasogastric intubation in an otherwise normal esophagus. Patients at high risk of this complication can be identified, and special techniques can be used to avoid perforation. Percutaneous endoscopic gastrostomy is an alternative to nasogastric intubation for long-term enteral feeding. When nasogastric intubation is performed, clinical tests of tube position are not sufficient to guarantee proper tube position. The tip of the tube must be visualized radiographically in the stomach prior to initiation of tube feeding. When perforation does occur, proper management is often controversial. Therapy must be tailored to each individual case for the best possible outcome.
Cellular, Biochemical, and Genetic Analysis of Mechanism of Small Molecule IAP Inhibitors (2004)

XIAP is member of the IAP family of anti-apoptotic proteins and is known for its ability to bind and suppress caspase family cell death proteases. A phenylurea series of chemical inhibitors of XIAP was recently generated by our laboratories (Schimmer, A. D., Welsh, K., Pinilla, C., Bonneau, M., Wang, Z., Pedersen, I. M., Scott, F. L., Glinsky, G. V., Scudiero, D. A., Sausville, E., Salvesen, G., Nefzi, A., Ostresh, J. M., Houghten, R. A., and Reed, J. C. (2004) Cancer Cell 5, 25-35). We examined the mechanisms of action of these chemical compounds using biochemical, molecular biological, and genetic methods. Active phenylurea-based compounds dissociated effector protease caspase-3 but not initiator protease caspase-9 from XIAP in vitro and restored caspase-3 but not caspase-9 enzymatic activity. When applied to tumor cell lines in culture, active phenylurea-based compounds induced apoptosis in a rapid, concentration-dependent manner, associated with activation of cellular caspases. Apoptosis induced by active phenylurea-based compounds was blocked by chemical inhibitors of caspases, with inhibitors of downstream effector caspases displaying more effective suppression than inhibitors of upstream initiator caspases. Phenylurea-based XIAP antagonists induced apoptosis (defined by annexin V staining) prior to mitochondrial membrane depolarization, in contrast to cytotoxic anticancer drugs. Consistent with these findings, apoptosis induced by phenylurea-based compounds was not altered by genetic alterations in the expression of Bcl-2 family proteins that control mitochondria-dependent cell death pathways, including over-expression of anti-apoptotic proteins Bcl-2 or Bcl-X(L) and genetic ablation of pro-apoptotic proteins Bax and Bak. Conversely, conditional over-expression of an active fragment of XIAP or genetic ablation of XIAP expression altered the apoptosis dose-response of the compounds. Altogether, these findings indicate that phenylurea-based XIAP antagonists block interaction of downstream effector caspases with XIAP, thus inducing apoptosis of tumor cell lines through a caspase-dependent, Bcl-2/Bax-independent mechanism.
A frequency-selective feedback model of auditory efferent suppression and its implications for the recognition of speech in noise (2012)

The potential contribution of the peripheral auditory efferent system to our understanding of speech in a background of competing noise was studied using a computer model of the auditory periphery and assessed using an automatic speech recognition system. A previous study had shown that a fixed efferent attenuation applied to all channels of a multi-channel model could improve the recognition of connected digit triplets in noise [G. J. Brown, R. T. Ferry, and R. Meddis, J. Acoust. Soc. Am. 127, 943-954 (2010)]. In the current study an anatomically justified feedback loop was used to automatically regulate separate attenuation values for each auditory channel. This arrangement resulted in a further enhancement of speech recognition over fixed-attenuation conditions. Comparisons between multi-talker babble and pink noise interference conditions suggest that the benefit originates from the model's ability to modify the amount of suppression in each channel separately according to the spectral shape of the interfering sounds.
Usability and security evaluation of GeoPass (2013)

We design, implement, and evaluate GeoPass: an interface for digital map-based authentication where a user chooses a place as his or her password (i.e., a "location-password"). We conducted a multi-session in-lab/at-home user study to evaluate the usability, memorability, and security of location-passwords created with GeoPass. The results of our user study found that 97% of users were able to remember their location-password over the span of 8-9 days and most without any failed login attempts. Users generally welcomed GeoPass; all of the users who completed the study reported that they would at least consider using GeoPass for some of their accounts. We also perform an in-depth usability and security analysis of location-passwords. Our security analysis includes the effect of information that could be gleaned from social engineering. The results of our security analysis show that location-passwords created with GeoPass can have reasonable security against online attacks, even when accounting for social engineering attacks. Based on our results, we suggest GeoPass would be most appropriate in contexts where logins occur infrequently, e.g., as an alternative to secondary authentication methods used for password resets, or for infrequently used online accounts.