gebhart/patrepeval huggingface dataset:
[Neural regulation of auto-grafted islets of Langerhans]. (1988)

The possibility of vagal reinnervation to intrasplenic islets grafts was examined by measuring portal insulin response to electric stimulation of the dorsal vagus nerve in autografted dogs. Grafted islets responded appropriately to methacholine given intravenously. However, no insulin secretory response could be observed in grafted dogs after vagal stimulation, which markedly increased portal insulin levels in control dogs. Therefore, intrasplenic grafted islets are not under direct vagal control. At the basal state, normal oscillatory release of insulin was observed in grafted animals, suggesting that the mechanism of rhythmic basal insulin release is intrinsic to the islet of Langerhans with no regulatory input from the vagus or any element of normal pancreatic structure.
deepDR: a network-based deep learning approach to<i>in silico</i>drug repositioning (2019)

Supplementary data are available online at Bioinformatics.
MHC II tetramers visualize human CD4+ T cell responses to Epstein–Barr virus infection and demonstrate atypical kinetics of the nuclear antigen EBNA1 response (2013)

Virus-specific CD4(+) T cells are key orchestrators of host responses to viral infection yet, compared with their CD8(+) T cell counterparts, remain poorly characterized at the single cell level. Here we use nine MHC II-epitope peptide tetramers to visualize human CD4(+) T cell responses to Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis (IM), a disease associated with large virus-specific CD8(+) T cell responses. We find that, while not approaching virus-specific CD8(+) T cell expansions in magnitude, activated CD4(+) T cells specific for epitopes in the latent antigen EBNA2 and four lytic cycle antigens are detected at high frequencies in acute IM blood. They then fall rapidly to values typical of life-long virus carriage where most tetramer-positive cells display conventional memory markers but some, unexpectedly, revert to a naive-like phenotype. In contrast CD4(+) T cell responses to EBNA1 epitopes are greatly delayed in IM patients, in line with the well-known but hitherto unexplained delay in EBNA1 IgG antibody responses. We present evidence from an in vitro system that may explain these unusual kinetics. Unlike other EBNAs and lytic cycle proteins, EBNA1 is not naturally released from EBV-infected cells as a source of antigen for CD4(+) T cell priming.
Genome Sequencing and Assembly (2011)

Decoding the genome sequence is becoming a fundamental tool for molecular, genetic, and genomic studies. This chapter reviews the history of DNA sequencing and technical principles of different sequencing platforms, and compares the strengths and weaknesses of different techniques for high-throughput genome sequencing applications are compared. It also covers brief descriptions on genome assembly and its validation.
Quantification of bacterial lipopolysaccharides (endotoxin) by GC–MS determination of 3-hydroxy fatty acids (2004)

A GC-MS method for the quantification of bacterial lipopolysaccharides (LPS, endotoxin) is presented. After hydrolytic cleavage of 3-hydroxy fatty acids (3-OH FAs) from the lipid A region of LPS, derivatisation of both the hydroxyl and the carboxyl group was performed in one step with a mixture of methyl-bis(trifluoracetamide)(MBTFA) and N-methyl-N-(tert-butyldimethylsilyl)trifluoracetamide (MTBSTFA). Using GC-MS in the EI mode with selected ion monitoring (SIM) for analysis, baseline separation of 3-OH FAs (and of possibly interfering 2-OH FAs) was achieved. The sensitivity of the method (LOD 7-50 pg/injection for the different 3-OH FAs investigated) allows for the efficient quantification of LPS in occupational and environmental samples. Degradation of 3-OH FAs as well as of their derivatives during sample preparation and GC-MS separation as a possible source of errors in analytical methods based on 3-OH FA determination is reported for the first time. Thermal elimination of water from the underivatised 3-OH FAs and of trifluoroacetic acid from the derivatives was identified as the cause of degradation. The resulting alpha,beta-unsaturated compounds showing the same mass spectra as the 3-OH FA derivatives were detected as more or less prominent satellite peaks. By using alkaline instead of acidic hydrolysis and cool on-column instead of split/splitless injection, elimination was reduced to an acceptable level.
Structure and regulation of the sheep metallothionein‐Ia gene (1986)

Screening of a sheep genomic lambda library with a sheep metallothionein-I cDNA clone resulted in the isolation of a 13,200-base-pair fragment containing a metallothionein gene which DNA sequence analysis identified as the gene encoding the cloned cDNA. The two introns occur at identical positions to those in other mammalian metallothioneins but are considerably larger. The first intron contains a DNA element that is present in a related but not identical form in many places in the sheep genome. Comparison of the promoter sequences of this gene (sMT-Ia) with the promoters of metallothionein genes from other species identified a number of conserved regions which may be important in the regulation of this gene by heavy metals, glucocorticoids and alpha-interferon. In sheep fibroblasts, the levels of sMT-Ia mRNA was found to be maximally elevated (95-fold) in the presence of zinc or cadmium and elevated 30-fold in the presence of copper. Dexamethasone had no effect upon mRNA levels. Thus this gene shows a pattern of regulation similar to the human MT-If gene, but distinct from the other human and mouse metallothionein genes so far reported.
Botulinum Toxin Type A Neuromuscular Blockade in the Treatment of Equinus Foot Deformity in Cerebral Palsy: A Multicenter, Open-Label Clinical Trial (2001)

BTX-A proved both safe and effective in the chronic management of focal muscle spasticity in children with equinus gait.
Genomic Databases and Resources at the National Center for Biotechnology Information (2009)

The National Center for Biotechnology Information (NCBI), as a primary public repository of genomic sequence data, collects and maintains enormous amounts of heterogeneous data. Data for genomes, genes, gene expressions, gene variation, gene families, proteins, and protein domains are integrated with the analytical, search, and retrieval resources through the NCBI Web site. Entrez, a text-based search and retrieval system, provides a fast and easy way to navigate across diverse biological databases.Customized genomic BLAST enables sequence similarity searches against a special collection of organism-specific sequence data and viewing the resulting alignments within a genomic context using NCBI's genome browser, Map Viewer.Comparative genome analysis tools lead to further understanding of evolutionary processes, quickening the pace of discovery.
WarpNet: Weakly Supervised Matching for Single-View Reconstruction (2016)

We present an approach to matching images of objects in fine-grained datasets without using part annotations, with an application to the challenging problem of weakly supervised single-view reconstruction. This is in contrast to prior works that require part annotations, since matching objects across class and pose variations is challenging with appearance features alone. We overcome this challenge through a novel deep learning architecture, WarpNet, that aligns an object in one image with a different object in another. We exploit the structure of the fine-grained dataset to create artificial data for training this network in an unsupervised-discriminative learning approach. The output of the network acts as a spatial prior that allows generalization at test time to match real images across variations in appearance, viewpoint and articulation. On the CUB-200-2011 dataset of bird categories, we improve the AP over an appearance-only network by 13.6%. We further demonstrate that our WarpNet matches, together with the structure of fine-grained datasets, allow single-view reconstructions with quality comparable to using annotated point correspondences.
Fiber Optic Rotary Joints-A Review (1982)

The rapid increase in the use of fiber optic signal transmission has spawned a need for "optical slip rings" to transfer optical signals across a rotating interface. Performance parameters that impact the development of these fiber optic rotary joints are investigated. The designs of existing single-channel fiber optic rotary joints are discussed with the intention of identifying design features that have the greatest impact on performance. The basic design feature of several multiple-channel fiber optic rotary joints is summarized.
Direct 3D Patterning of TiO<sub>2</sub> Using Femtosecond Laser Pulses (2007)

Direct 3D patterning of TiO2 with femtosecond laser pulses using a sol–gel-based negative-tone spin-coatable TiO2 resist is demonstrated (see figure). A layer of the resist was spun on glass and patterned in three dimensions by exposing it to femtosecond laser pulses and then developing in acetone. Heat treatment at 400 °C in air gave 3D patterns that were ca. 50 % of their original size.
Synthesis of nanocrystalline rutile-phase titania at low temperatures (2009)

Submitted by Lemos Marina (marinalemos@id.uff.br) on 2015-07-31T12:52:46Z No. of bitstreams: 1 Synthesis of nanocrystalline.pdf: 846505 bytes, checksum: e0bfa042f5d9f12576ff2c8bc506f358 (MD5)
Factors influencing interocular blur suppression (1994)

An Important feauture of monovision correction is the ability of the binocular system to suppress blur from the defocused eye (interocular blur suppression). We conducted a study with three subjects and found that interocular blur suppression improved with higher levels of monocular defocus (addition powers in monovision) but was not affected by the eye defocused (sighting dominant or non‐domninant) or by pupil size. The selection and management of monovision patients may be assisted by an understading of the understanding of the underlying suppression preocesses.
A Novel Metamorphic Mechanism for Efficient Additive Manufacturing (2014)

A novel metamorphic rolling mechanism (MRM) based on genetic evolution synthesis that realized multiway, multiple-DOF and synchronous plastic forming in arc based deposition processing of components with variable wall thickness is proposed in this paper. Topology and mechanical structure of the metamorphic mechanism is presented and D-H equation is derived. Experiments show that firstly efficient manufacturing of components with variable wall thickness can be realized by synchronous rolling in vertical direction. Secondly precision and surface configurations of the components are improved compared with that of freeform deposition manufacturing. The metamorphic mechanism provides possibilities of efficient hybrid manufacturing of near net shape components with variable wall thickness.
Distance-classifier correlation filters for multiclass target recognition (1996)

We describe a correlation-based distance-classifier scheme for the recognition and the classification of multiple classes. The underlying theory uses shift-invariant filters to compute distances between the input image and ideal references under an optimum transformation. The original distance-classifier correlation filter was developed for a two-class problem. We introduce a distance-classifier correlation filter that simultaneously considers multiple classes, and we show that the earlier two-class formulation is a special case of the classifier presented. Initial results are presented to demonstrate the discrimination- and distortion-tolerance capabilities of the proposed filter.
Comparison of the effective conductivity between composites reinforced by graphene nanosheets and carbon nanotubes (2008)

Both graphene nanosheets and carbon nanotubes have exceptional electric and thermal conductivities, rendering them excellent candidates as second-phase fillers in composite materials to substantially enhance their effective conductivities. Their markedly different geometries, however, can have significant effect on the effective conductivities of composites, which we investigate using an effective medium approximation. It is demonstrated that graphene nanosheet is more effective in conductivity enhancement than carbon nantubes, and both fillers lead to substantially higher conductivity and much reduced percolation threshold in composites. The effects of conductivity anisotropy and interfacial resistance are also discussed.
1310-nm Hybrid III–V/Si Fabry–Pérot Laser Based on Adhesive Bonding (2011)

An evanescently coupled, hybrid III-V/Silicon Fabry-Pérot laser based on adhesive divinyl siloxane-benzocyclobutene (DVS-BCB) bonding is presented operating at 1310 nm. We obtain 5.2-mW output power in continuous-wave (CW) regime at 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> C with a threshold current density of 2.83 kA/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> in an 800-μm -long device. A specially developed bonding procedure produces 50-nm-thick bonding layers, enabling the evanescent coupling.
Expression of a gene encoding a glycine-rich protein in petunia. (1987)

We have investigated the expression of a gene that codes for a glycine-rich structural protein (GRP1) in petunia. This gene is expressed as a single polyadenylated RNA of approximately 1,600 bases which was found to be present in leaves, stems, and flowers of petunia but not in roots. In the organs in which GRP1-specific mRNA was expressed, its steady-state levels were highest in stems and leaves and lowest in flowers. This analysis also revealed that the pattern of organ-specific expression for several of the GRP1-related genes was distinctly different. In addition, it was found that the levels of GRP1 RNA were significantly higher in young leaves and stems than in old, implying developmental regulation of the gene. GRP1-specific RNA in both old and young tissue that had been wounded was found to be increased at least 25-fold over that in young unwounded tissue. Increased levels of GRP1 mRNA were seen within 5 min after wounding, with substantial increases apparent by 30 min. Maximal levels of accumulation of GRP1 transcripts occurred 90 min after wounding. The enhancement of GRP1 mRNA levels by wounding appears to be one of the earliest events of the plant wound response and is distinct from that which we observed for the PAL gene in petunia. Using S1 analysis and RNA primer extension, we demonstrated that the same transcriptional start site was used by the GRP1 gene in all organs and in wounded and unwounded tissue. The potential significance of these data with regard to wound signal transduction is discussed.
A BiCMOS channelless masterslice with on-chip voltage converter (2003)

An approach to solving reliability problems due to voltage tolerance in submicron devices is described which involves lowering the operating voltage using an on-chip voltage conversion system. The masterslice has an on-chip voltage converter, TLL-compatible I/O circuits and uses advanced 0.8- mu m BiCMOS technology. A cross-sectional view of the BiCMOS device, which consists of CMOS transistors with simple n-well structure and an n-p-n bipolar transistor, is shown. The masterslice has been applied to a digital signal processing circuit. The chip contains 20 k gates, including four multiplier macrocells and random logic circuits. In the random logic circuits, which consist of double-size transistors, the packing density is 240 gate/mm/sup 2/, and the average delay is 600 ps for a two-input NAND gate with FO=3 and 1-mm wiring length. In the multipliers, 660 gate/mm/sup 2/ packing density and 450 ps average delay for 2-input NAND gate with FO=3 are attained.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Integrated Velocity Model Estimation For Accurate Imaging (2004)

Abstract Surface seismic reflections, surface seismic direct arrivals, well data and prior geologic information can be used to constrain a subsurface velocity model.
Proliferation of Myoblast Skeletal Cells on Three-Dimensional Supermacroporous Cryogels (2010)

Cardiac and skeletal muscle tissue engineering provides a smart approach to overcome problems associated with organ transplantation and cardiac tissue and also lays a platform for superior alternative approaches in muscle regeneration. The aim of the study was to demonstrate cryogel scaffold potential in the field of skeletal muscle and cardiac tissue engineering. Poly-hydroxyethyl methacrylate (pHEMA)-gelatin cryogel scaffold was synthesized using cryogelation technique and such a designed material is being reported first time. Rheology study of the pHEMA-gelatin (HG) suggested that the cryogel scaffolds were stable at different temperatures and phase angle remained constant in both dry and wet state. HG cryogel was able to bear increased stress without leading to deformation. Monitoring the hydration of HG scaffold showed shift from a stiff to a more pliable material and upon continuing hydration, shear modulus remained constant with no further change observed. However, the change in phase angle <0.24 masculine indicates a gradual increase in stiffness of the material over time. Scaffold synthesised using such polymer combinations gave cells a native environment for proliferation and surface stiffness have shown to help in differentiation of the cells. Myoskeletal cell lines were cultured on these scaffolds to check the biocompatibility and cell proliferation. Alamar blue assay performed over a period of 3 weeks analysed the metabolic activity of cells which showed more than 60% increase in the total cellular activity. DNA content of cells was found to be directly related to number of cells present at a given time point and this was found to have increased by more than 50% in 3 weeks. Since in 3-D scaffold the surface area is more in comparison to 2-D, hence better cell proliferation is observed. Hoechst and DAPI staining showed tubular structure and alignment of the cells during formation of the tubules shows promising cellular response to the cryogel matrix. The mechanical strength, stiffness and elastic measurements of the scaffold indicated potential application of these materials for skeletal and cardiac tissue engineering.
Lysine and ornithine analogs of methotrexate as inhibitors of dihydrofolate reductase (1982)

The ornithine (6a) and lysine (6b) analogues of methotrexate (1) have been synthesized via condensation of 4-amino-4-deoxy-N10-methylpteroic acid (2) with N gamma-carbobenzoxy-L-ornithine tert-butyl ester (3a) and N epsilon-carbobenzoxy-L-lysine tert-butyl ester (3b), respectively. Removal of the protecting groups gave 5a and 6b. Compounds 6a and 6b and their precursor Cbz acids (5a and 5b) show significant inhibition of dihydrofolate reductase.
Colostomy Control Device (1986)

Temporary or permanent colostomy produces many difficulties such as continuous fecal flow, odor, skin irritation, rash, edema, and uncleanliness. Efforts to resolve these difficulties have not had much success. The present work describes the development of a new colostomy control device and its successful application to eight patients. The device is removable and allows the patients to empty their bowels naturally. Following the bowel movement and cleaning of the stoma, the device is reapplied. The patient remains continent and experiences a psychological uplift from being able to feel the urge for bowel movements. The application of the device is easy and leads to no complications.
Stable free radicals. III. 1,3-dioxy-2-imidazolidone zwitterion and its stable nitronyl nitroxide radical anion (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStable free radicals. III. 1,3-dioxy-2-imidazolidone zwitterion and its stable nitronyl nitroxide radical anionD. G. B. Boocock and Edwin F. UllmanCite this: J. Am. Chem. Soc. 1968, 90, 24, 6873–6874Publication Date (Print):November 1, 1968Publication History Published online1 May 2002Published inissue 1 November 1968https://doi.org/10.1021/ja01026a072RIGHTS & PERMISSIONSArticle Views443Altmetric-Citations44LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (265 KB) Get e-Alerts Get e-Alerts
I Write Her Name (2007)

A Romanian girl approaches me while I peruse a menu in Sibiu, politely asks if I am Chinese...
Microelectromechanical-Systems-Based Switches for Power Applications (2012)

A new system for switching electrical power using microelectromechanical systems (MEMS) is presented. The heart of the system utilizes custom-designed MEMS switching device arrays that are able to conduct current more efficiently and can open orders of magnitude faster than traditional macroscopic mechanical relays. Up to now, MEMS switches have been recognized for their ability to switch very quickly due to their low mass but have only been used to carry and switch very low currents at extremely low voltage. However, recent developments have enabled suppression of the arc that normally occurs when the MEMS switch is opened while current is flowing. The combination of the arc suppression with the MEMS switch arrays designed for this purpose enables a breakthrough increase in current and voltage handling capability. The resultant technology has been scaled to handle many amperes of current and switch hundreds of volts. Such current and voltage handling capability deliver improved energy efficiency and the capacity to handle fault current levels that are encountered in typical ac or dc power systems. Fault current interruption takes place in less than 10 μs, almost regardless of the prospective fault current magnitude. The properties of the MEMS switch arrays allow the switching mechanism to operate at temperatures in excess of 200 °C . The switches also have a vibration tolerance in excess of 1000 G. The combination of fast MEMS switching speed, optimized current and voltage handling capacity of the switch arrays, the arc suppression circuitry, and optimized sensing and control enables a single sensing, control, and switching system to operate in a small fraction of a millisecond. This paper will present the basic physics of the MEMS switches together with recent advances that enable the technology. Some illustrative examples of the ways that the devices may be used to provide protection and control within electrical systems will also be presented.