gebhart/patrepeval huggingface dataset:
Expression of E2A-HLF Chimeric Protein Induced T-Cell Apoptosis, B-Cell Maturation Arrest, and Development of Acute Lymphoblastic Leukemia (1999)

The E2A-HLF fusion gene, generated by t(17;19)(q22;p13) in acute lymphoblastic leukemia (ALL), encodes a chimeric transcription factor in which the trans-activating domains of E2A are fused to the DNA-binding and dimerization domains of hepatic leukemic factor (HLF). To investigate its biological role, we generated transgenic mice expressing E2A-HLF using Ig enhancer and promoter, which direct transgene expression in cells committed to the lymphoid lineage. The transgenic mice exhibited abnormal development in the thymus and spleen and were susceptible to infection. The thymus contained small numbers of thymocytes, and TUNEL staining showed that higher population of thymocytes were undergoing apoptosis. The spleen exhibited a marked reduction in splenic lymphocytes and the flow cytometric analyses and the in vitro colony formation assays showed that the B-cell maturation was blocked at a very early developmental stage. These findings indicated that the expression of E2A-HLF induced T-cell apoptosis and B-cell maturation arrest in vivo and that the susceptibility of the transgenic mice to infection was due to immunodeficiency. Moreover, several transgenic mice developed acute leukemia, classified as T-ALL based on the surface marker analysis and DNA rearrangements, suggesting that an additional event is required for malignant transformation of lymphoid cells expressing E2A-HLF. Our findings provide insight into the biological function of E2A-HLF in lymphoid development and also its role in leukemogenesis.
Overview of speech enhancement techniques for automatic speaker recognition (2002)

Real world conditions differ from ideal or laboratory conditions, causing mismatch between training and testing phases, and consequently, inducing performance degradation in automatic speaker recognition systems. Many strategies have been adopted to cope with acoustical degradation; in some applications of speaker identification systems a clean sample of speech, prior to the recognition stage, is needed. This has justified the use of procedures that may reduce the impact of acoustical noise on the desired signal, giving rise to techniques involved in the enhancement of noisy speech. A comparative performance analysis of single-channel (based in classical spectral subtraction and some derived alternatives), dual-channel (based in adaptive noise cancelling) and multi-channel (using microphone arrays) speech enhancement techniques, with different types of noise at different SNRs, as a pre-processing stage to an ergodic HMM-based speaker recognizer, is presented.
Interleukin 12 at the site of disease in tuberculosis. (1994)

Interleukin 12 (IL-12), a heterodimeric cytokine composed of p40 and p35 chains, has potent immunologic effects in vitro. We used tuberculous pleuritis as a model to study the immunoregulatory potential of IL-12 in vivo at the site of human infectious disease. Messenger RNAs for p40 and p35 were detected in pleural fluid from six of six patients by reverse-transcription polymerase chain reaction. By using an ELISA that detected both free p40 and heterodimeric IL-12, we found that mean concentrations were 585 +/- 89 pg/ml in pleural fluid of patients with tuberculous pleuritis, which were significantly higher than those in serum of the same patients (54 +/- 36 pg/ml), or in malignant pleural effusions (123 +/- 35 pg/ml). By using an ELISA specific for heterodimeric IL-12, we found that mean concentrations in pleural fluid of patients with tuberculous pleuritis were 165 +/- 28 pg/ml and undetectable in serum of the same patients, or in malignant pleural effusions. Bioactive IL-12 was detectable in five of five supernatants of pleural fluid cells stimulated with Mycobacterium tuberculosis. Addition of anti-IL-12 antibodies suppressed proliferative responses of pleural fluid cells to M. tuberculosis by 36 +/- 7%. These data indicate that IL-12 may play a role in the human immune response to infectious agents in vivo. We hypothesize that IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.
High H<sub>2</sub> Adsorption in a Microporous Metal–Organic Framework with Open Metal Sites (2005)

Ein auf Kupfer basierendes metall-organisches Gerüst (MOF) wurde synthetisiert und seine Struktur röntgenographisch ermittelt (siehe Bild; Cu blau, C schwarz, O rot, Gerüstpore gelb). Des Weiteren wurde die Wasserstoffadsorption durch diese Verbindung untersucht, die mit 2.47 Gew.-% ungewöhnlich hoch ist.
The psychiatric side-effects of rimonabant (2009)

Patients taking CB1 receptor antagonists should be carefully investigated for psychiatric side-effects. These drugs should not be prescribed for those already suffering from mental disorders. Nevertheless, the development of new compounds targeting the endocannabinoid system for the treatment of several conditions would be necessary and opportune.
Propofol-Induced Alpha Rhythm (2004)

The electroencephalographic effects of two intravenous sedative/hypnotic drugs, propofol and thiopental, were studied at three stable blood concentrations in 52 normal healthy volunteers. The higher concentration resulted in unresponsiveness (lack of response to auditory/tactile stimuli) in all subjects. This report describes the strong frontal-central rhythms apparent in this state using a quantitative description of oscillatory systems underlying the rhythm. These rhythms occur when sedative drug concentrations are greater than those producing the well-described increase in broadband beta-power associated with many sedative drugs. Propofol induces rhythms in the alpha-range, while thiopental produces rhythms in the beta-range. Quasistationary for a period of about 1 h, these rhythms exceed the baseline alpha-rhythm in power. By their resonant nature, these propofol-induced rhythms are analogous to 'the classic alpha-rhythm', but quantitative characteristics of the underlying oscillatory systems are different. Baseline properties of the oscillatory system underlying the initial resting alpha-rhythm recover completely as drug concentration decays to negligible values.
RAMS: A VHDL-AMS Code Refactoring Tool Supporting High Level Analog Synthesis (2005)

In this paper, a code refactoring methodology for the high-level analog synthesis is presented. It restructures, refines, and simplifies an analog behavioral model written in VHDL-AMS. Through code refactoring one improves the comprehensibility, expandability and reusability of the behavioral model and brings the model to a necessary preliminary stage for the actual circuit synthesis. This approach supports the top-down hierarchical design flow for analog and mixed-signal application.
Applications of Patient-Specific Induced Pluripotent Stem Cells; Focused on Disease Modeling, Drug Screening and Therapeutic Potentials for Liver Disease (2010)

The recent advances in the induced pluripotent stem cell (iPSC) research have significantly changed our perspectives on regenerative medicine by providing researchers with a unique tool to derive disease-specific stem cells for study. In this review, we describe the human iPSC generation from developmentally diverse origins (i.e. endoderm-, mesoderm-, and ectoderm- tissue derived human iPSCs) and multistage hepatic differentiation protocols, and discuss both basic and clinical applications of these cells including disease modeling, drug toxicity screening/drug discovery, gene therapy and cell replacement therapy.
Molybdenum Cofactor Biosynthesis in Humans:  Identification of a Persulfide Group in the Rhodanese-like Domain of MOCS3 by Mass Spectrometry (2005)

The human MOCS3 protein contains an N-terminal domain similar to the Escherichia coli MoeB protein and a C-terminal segment displaying similarities to the sulfurtransferase rhodanese. MOCS3 is proposed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C-terminus of the smaller subunit of molybdopterin (MPT) synthase during Moco biosynthesis in humans. Recent studies have shown that the MOCS3 rhodanese-like domain (MOCS3-RLD) catalyzes the transfer of sulfur from thiosulfate to cyanide and is also able to provide the sulfur for the thiocarboxylation of MOCS2A in a defined in vitro system for the generation of MPT from precursor Z. MOCS3-RLD contains four cysteine residues of which only C412 in the six amino acid active loop is conserved in homologous proteins from other organisms. ESI-MS/MS studies gave direct evidence for the formation of a persulfide group that is exclusively formed on C412. Simultaneous mutagenesis of the remaining three cysteine residues showed that none of them is involved in the sulfur transfer reaction in vitro. A disulfide bridge was identified to be formed between C316 and C324, and possible roles of the three noncatalytic cysteine residues are discussed. By ESI-MS/MS a partially gluconoylated N-terminus of the His6-tagged MOCS3-RLD was identified (mass increment of 178 Da) which resulted in a heterogeneity of the protein but did not influence sulfurtransferase activity.
Structural Aspects of Palladium and Platinum Complexes With Chiral Diphosphinoferrocenes Relevant to the Regio- and Stereoselective Copolymerization of CO with Propene (2001)

A series of chiral diphosphinoferrocene ligands 3a – i, derived from josiphos (=(2R)-1-[(1R)-1-(dicyclohexylphosphino)ethyl]-2-(diphenylphosphino)ferrocene, formerly called {(R)-1-[(S)-2-(diphenylphosphino)ferrocenyl]ethyl}dicycloxexylphosphine) where the electronic properties of the ligand are systematically varied, were prepared. X-Ray studies of five of these new ligands confirmed that these compounds display very similar conformations in the solid state and that no structural criteria could be found indicating the modified electronic properties. These ligands find application in the Pd-catalyzed highly regio- and stereoselective CO/propene copolymerization reaction, where the electronic properties of the ligand show a great impact on the catalyst activity. Coordination-chemical aspects of these diphosphinoferrocenes relevant to the CO/propene copolymerization reaction were addressed by the preparation and characterization of Pd- and Pt-complexes of the general formula [PdCl2(P−P)] (5), [PdMe2(P−P)] (6), [PdClMe(P−P)] (7), [PdMe(MeCN)(P−P)]PF6 (8), and [PtClMe(P−P)] (9) (P−P=chiral diphosphinoferrocene ligand (3a – h), four of which were characterized by X-ray crystallography.
Thermogravimetric analysis. A review (1963)

The first page of this article is displayed as the abstract.
Risk analysis and evaluation of capital investment projects (2001)

In determining the feasibility of projects where capital investments are concerned, various methods are used. The focus of these methods is on return per se, so it is often asked to what extent any of these methods take the risk concept into account. The main objective of this study was to investigate the importance of risk with regard to capital investment projects. Secondly, with the aid of an empirical study, the study tried to establish whether risk is incorporated when South African companies evaluate capital investment projects. The empirical analysis indicated that risk analysis and evaluation in practice are to a large extent neglected by South African companies. It was found that nearly a quarter of companies estimate their annual cash flows using management subjective estimates alone.
Dynamic and Precise Visualization of Contrast Agent in Blood Vessels with Motion Correction (2006)

We have developed a new ultrasound imaging method for the visualization of vascularity with less than 500 µm in width applicable to tissues naturally moving caused by respiration or heart beats. In our method, we utilize the least square method based on tissue-motion measurement method previously reported, which divides an image into extremely small regions so that the loss of correlation caused by tissue motion can be virtually ignored. We averaged images of tissues with contrast agents after correcting for the tissue motion on the basis of measurement results. In vivo results of experiments using rabbit kidneys revealed that the vascularity including peripheral vessels, e.g., those in the renal cortex, could be clearly imaged without any motion artifact by averaging more than 30 frames. Moreover, it was indicated that a pseudo-three-dimensional imaging of small vessels can be achieved by this method utilizing slight tissue motions in the slice direction during obtaining frames to be averaged. In another experiment using tumor-implanted rabbits, it was also found that neovascularity in a tumor tissue could be identified more clearly on a time-averaged image than on a conventional image.
Stability of antimicrobial agents in peritoneal dialysate (1982)

The stability of cephapirin, gentamicin, penicillin G, nafcillin, ticarcillin, and vancomycin was tested in peritoneal dialysate at 25 degrees C for 24 h. All of the antimicrobial agents were stable except penicillin G, which lost 25% of activity over 24 h (P less than 0.01). The once-daily preparation of drug-dialysate solution is feasible for the treatment of peritonitis in patients on continuous ambulatory peritoneal dialysis.
Pasteurella multocida toxin induces IL-6, but not IL-1 alpha or TNF alpha in fibroblasts. (1995)

Pasteurella multocida toxin (PMT) is the major virulence factor in Progressive Atrophic Rhinitis of swine. Other workers' previous findings that PMT was mitogenic for 3T3 fibroblasts, were confirmed in the present study. In addition, PMT stimulated 3T3 cells to release IL-6, but IL-1 alpha or TNF alpha were not detected in fibroblast supernatants sampled 24, 48, or 72 h after stimulation. In view of the role of IL-6 in osteoclastic bone resorption, these findings provide a new working hypothesis for investigations into the molecular pathogenesis of this important disease.
High-speed operation of LiNbO_3 electro-optic interferometric waveguide modulators (1980)

High-speed optical intensity modulation is reported for the first time using single-mode electro-optic interferometric waveguide modulators formed from Ti-diffused waveguides in LiNbO3. For cw 0.633-microm optical inputs, modulation at rates up to 1.4 GHz has been achieved by driving the interferometer through multiple-7r phase shifts. Envelope modulation of a 275-MHz optical pulse train from a cw mode-locked frequency-doubled Nd:YAG laser with a 68.9-MHz drive signal has also been demonstrated.
Tuning the DNA Reactivity of <i>cis</i>-Platinum:  Conjugation to a Mismatch-Specific Metallointercalator (2004)

A novel bimetallic conjugate combining a rhodium intercalator that selectively binds DNA mismatches and a reactive cis-platinum analogue that targets DNA by coordination has been prepared. The site-selectivity of the bimetallic complex in forming coordination adducts is examined using mismatched and well-matched oligonucleotides of different sequences. The results indicate that through the bifunctional complex, the platinum center can be targeted near mismatched sites. Interestingly, with mismatched, DNA both intrastrand and the less common interstrand cross-linked adducts are formed. The recognition of a DNA mismatch by the bulky Rh intercalator appears to direct the Pt unit, depending upon steric contraints, to react preferentially with mismatched DNA at a site that may or may not be the preferred site of Pt coordination. Thus, the presence of a permanent link to a site-specific intercalator is able to tune the reactivity of the cis-platinum analogue.
The theory of dynamic programming (1954)

Abstract : The paper is the text of an invited address before the annual summer meeting of the American Mathematical Society at Laramie, Wyoming, September 2, 1954. The contents are chiefly of an expository nature on the theory of dynamic programming.
Human <scp>ALPI</scp> deficiency causes inflammatory bowel disease and highlights a key mechanism of gut homeostasis (2018)

Herein, we report the first identification of biallelic-inherited mutations in <i>ALPI</i> as a Mendelian cause of inflammatory bowel disease in two unrelated patients. <i>ALPI</i> encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. Prediction tools and structural modelling indicate that all mutations affect critical residues or inter-subunit interactions, and heterologous expression in HEK293T cells demonstrated that all <i>ALPI</i> mutations were loss of function. <i>ALPI</i> mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. Furthermore, ALPI expression was reduced in patients' biopsies, and ALPI activity was undetectable in ALPI-deficient patient's stool. Our findings highlight the crucial role of ALPI in regulating host-microbiota interactions and restraining host inflammatory responses. These results indicate that <i>ALPI</i> mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Towards closing the capacity gap on multiple antenna channels (2002)

In recent years, soft iterative decoding techniques have been shown to greatly improve the bit error rate performance of various communication systems. For multiple antenna systems, however, it is not clear what is the best way to obtain the soft-information required of the iterative scheme with low complexity. In this paper, we propose a modification of the Fincke-Pohst (sphere decoder) algorithm to estimate the MAP probability of the received symbol sequence. The new algorithm solves a nonlinear integer leasts-quares problem and, over a wide range of rates and SNRs, has polynomial-time (often cubic) complexity. The performance of the algorithm, combined with convolutional, turbo, and LDPC codes is demonstrated on several multiple antenna channels.
Selectivity in liquid chromatography with micellar mobile phases (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSelectivity in liquid chromatography with micellar mobile phasesPaul. Yarmchuk, Robert. Weinberger, R. F. Hirsch, and L. J. Cline. LoveCite this: Anal. Chem. 1982, 54, 13, 2233–2238Publication Date (Print):November 1, 1982Publication History Published online1 May 2002Published inissue 1 November 1982https://pubs.acs.org/doi/10.1021/ac00250a023https://doi.org/10.1021/ac00250a023research-articleACS PublicationsRequest reuse permissionsArticle Views114Altmetric-Citations68LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Osteoclast precursors in bone marrow and peritoneal cavity (1997)

Osteoclasts differentiate from cells that share some phenotypes with mature macrophages and monocytes, but early precursors for osteoclasts still remain obscure. To characterize osteoclast precursors, using monoclonal anti-c-Fms and anti-c-Kit antibodies, bone marrow cells were separated and the frequency of clonogenic progenitors were measured. Osteoclast precursors in the bone marrow mainly expressed c-Kit and diminished in frequency when they expressed c-Fms. In contrast to bone marrow, the precursors in the peritoneal cavity were enriched with a population of c-Fms+. Injection of these antibodies into mice demonstrated that peritoneal osteoclast precursors were sensitive to anti-c-Fms but not to anti-c-Kit antibodies, whereas those in bone marrow only declined in the presence of both antibodies. Meanwhile, c-Fms as opposed to c-Kit played an essential role in the generation of osteoclasts in cultures. We also compared osteoclast precursors with colony forming cells (CFU-M) by a macrophage colony stimulating factor. CFU-M in bone marrow decreased when anti-c-Kit antibody was administered and no CFU-M was detected in peritoneum. In this study, we show differences between proliferative potential osteoclast precursors maintained in bone marrow and peritoneum and between CFU-M and osteoclast precursors.
Systematic mutational analysis of cAMP-dependent protein kinase identifies unregulated catalytic subunits and defines regions important for the recognition of the regulatory subunit. (1992)

A library of mutants of the catalytic subunit of the Saccharomyces cerevisiae cAMP-dependent protein kinase was screened in vitro for mutants defective in the recognition of the regulatory subunit. The mutations identified were mapped onto the three-dimensional structure of the mouse catalytic subunit with a peptide inhibitor. Mutations defective in the recognition of both the regulatory subunit and the peptide substrate Leu-Arg-Arg-Ala-Ser-Leu-Gly (Kemptide) mapped to the peptide-binding site shared by all substrates and inhibitors of the catalytic subunit and functionally define the binding site for the autoinhibitor sequence in the hinge region of the regulatory subunit. Mutants defective only in the recognition of the regulatory subunit identified residues that comprise additional binding sites for the regulatory subunit. The majority of these residues are clustered on the surface of the catalytic subunit in a region flanking the distal portion of the autoinhibitor/peptide-binding site. The simultaneous substitution of Lys233, Asp237, Lys257, and Lys261 in this region caused a 260-fold decrease in affinity for the regulatory subunit, whereas the catalytic efficiency toward Kemptide decreased by only 1.8-fold. The substitution of autophosphorylated Thr241, also in this region, and the 3 residues interacting with the phosphate also caused an unregulated phenotype.
A Bioartificial Liver to Treat Severe Acute Liver Failure (1994)

This bioartificial liver is safe and serves as an effective "bridge" to liver transplant in some patients.
Simultaneous depth and area minimization in LUT-based FPGA mapping (1995)

In this paper, we present an improvement of the FlowMap algorithm, named CutMap, which combines depth and area minimization during the mapping process by computing min-cost min-height K-feasible cuts for critical nodes for depth minimization and computing min-cost K-feasible cuts for non-critical nodes for area minimization. CutMap guarantees depth-optimal mapping solutions in polynomial time as the FlowMap algorithm but uses considerably fewer K-Luts. We have implemented CutMap and tested it on the MCNC logic synthesis benchmarks. For depth-optimal mapping solutions, CutMap uses 15% fewer K-LUTs than FlowMap. We also tested CutMap followed by the depth relaxation routines in FlowMap_r algorithm, which achieves area minimization by depth relaxation. CutMap followed FlowMap_r performs better than FlowMap_r.