gebhart/patrepeval huggingface dataset:
Expression and preliminary characterization of recombinant human tissue kallikrein in egg white of laying hens (2006)

Human tissue kallikrein (hK1) plays an important role in regulation of blood pressure, electrolyte and glucose transport, and renal function. To evaluate the feasibility of expression of recombinant human tissue kallikrein (rhK1) in the egg whites of laying hens, human tissue kallikrein gene (hKLK1) cDNA was subcloned into the chicken oviduct-specific expression vector (pOV3), and the resultant recombinant vector pOV3K was injected into laying hens via wing vein after mixing with polyethyleneimine. Following injection twice with the recombinant vector, the enzymatic activity at a maximal level of 59 U/mL was detected in the egg whites, which lasted for more than 7 d. The expression level of rhK1 in the egg whites in the 3-mg group was relatively higher than that in the 2-mg group, but the significant differences were identified on d 7 and 8 (P < 0.05). Ten days after the primary injection, the hens were reinjected with the same dose of the vector, and even higher enzymatic activity was detected in their egg whites. Two different breeds of hen were tested with no difference in expression level found (P > 0.05). Western blot analysis of the egg whites from vector-injected hens showed the rhK1 was recognized by a polyclonal antibody specific for hK1 with molecular weights of 37 and 43 kDa, which probably corresponded to the mature and preenzyme, respectively. Biochemical studies showed that the recombinant enzyme had a similar thermostability, optimal pH, hypotensive effect, and sensitivity to different ions to the natural enzymes in human and porcine tissues. These data indicate that the chicken oviduct-specific transient expression system can produce relatively high level and authentic recombinant enzyme with a potential for further development for therapeutic use.
HMM based Archive Film Defect Detection with Spatial and Temporal Constraints (2009)

We propose a novel probabilistic approach to detect defects in digitized archive film, by combining temporal and spatial information across a number of frames. An HMM is trained for normal observation sequences and then applied within a framework to detect defective pixels by examining each new observation sequence and its subformations via a leave-one-out process. A two-stage false alarm elimination process is then applied on the resulting defect maps, comprising MRF modelling and localised feature tracking, which impose spatial and temporal constraints respectively. The proposed method is compared against state-of-the-art and industry-standard methods to demonstrate its superior detection rate.
Système d’inspection automatique de surface SIAS (1996)

Sollac, l’Irsid et MCS Matra ont développé en commun un système d’inspection automatique de défauts de surface à grande vitesse de traitement. Ce système, dont la configuration évolutive permet de s’adapter à tous types de produits, a été bâti à partir du cahier des charges des experts de contrôle qualité. Une attention particulière a donc été portée à la facilité d’apprentissage et de mise au point de la classification, à la quantification objective de la qualité de surface indépendamment de la sévérité d’aspect et à l’intégration dans la politique de contrôle qualité tout au long de la filière de production.
The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity (2005)

We have recently described an evolutionarily conserved protein motif, designated the THAP domain, which defines a previously uncharacterized family of cellular factors (THAP proteins). The THAP domain exhibits similarities to the site-specific DNA-binding domain of Drosophila P element transposase, including a putative metal-coordinating C2CH signature (CX(2-4)CX(35-53)CX(2)H). In this article, we report a comprehensive list of approximately 100 distinct THAP proteins in model animal organisms, including human nuclear proapoptotic factors THAP1 and DAP4/THAP0, transcriptional repressor THAP7, zebrafish orthologue of cell cycle regulator E2F6, and Caenorhabditis elegans chromatin-associated protein HIM-17 and cell-cycle regulators LIN-36 and LIN-15B. In addition, we demonstrate the biochemical function of the THAP domain as a zinc-dependent sequence-specific DNA-binding domain belonging to the zinc-finger superfamily. In vitro binding-site selection allowed us to identify an 11-nucleotide consensus DNA-binding sequence specifically recognized by the THAP domain of human THAP1. Mutations of single nucleotide positions in this sequence abrogated THAP-domain binding. Experiments with the zinc chelator 1,10-o-phenanthroline revealed that the THAP domain is a zinc-dependent DNA-binding domain. Site-directed mutagenesis of single cysteine or histidine residues supported a role for the C2CH motif in zinc coordination and DNA-binding activity. The four other conserved residues (P, W, F, and P), which define the THAP consensus sequence, were also found to be required for DNA binding. Together with previous genetic data obtained in C. elegans, our results suggest that cellular THAP proteins may function as zinc-dependent sequence-specific DNA-binding factors with roles in proliferation, apoptosis, cell cycle, chromosome segregation, chromatin modification, and transcriptional regulation.
Scanning electron microscopy observation of excitation of the surface acoustic waves by the regular domain structures in the LiNbO3 crystals (1992)

This letter reports a scanning electron microscope study of excitation of surface acoustic waves by the regular domain structures in LiNbO3 crystals. The regular domain structures in LiNbO3 crystals were formed by the method of aftergrowth thermoelectrical treatment. It is shown that the regular domain structures excite surface acoustic waves by the piezoelectric effect. In this case, the wavelength of the surface acoustic waves is determined by the period of the regular domain structure. These experiments have been made at CNRS (Grenoble, France) using a scanning electron microscope JSM-840.
Autocorrelation Complementary Matrices (2019)

This paper studies autocorrelation complementary matrices (ACM), a set of N matrices of size M × L whose two-dimensional autocorrelations add to a two-dimensional delta-function, which we refer to as a set of (M, N, L)-ACM. The ACM is an extension of the Golay complementary sequences to a matrix form. In addition to an existing approach in the literature to construct the ACM, we show that i) a set of (M, N, L)-complete complementary codes (CCC) is actually a set of (M, N, L)-ACM, and ii) from two pairs of complementary sequences of length M and L we can construct a set of (M, 4, L)-ACM (i.e., N = 4). As an application of the ACM, we show how it can be applied to omnidirectional precoding for a multi-input multi-output (MIMO) communication system with a uniform rectangular array (URA).
Clec4A4 is a regulatory receptor for dendritic cells that impairs inflammation and T-cell immunity (2016)

Dendritic cells (DCs) comprise several subsets that are critically involved in the initiation and regulation of immunity. Clec4A4/DC immunoreceptor 2 (DCIR2) is a C-type lectin receptor (CLR) exclusively expressed on CD8α(-) conventional DCs (cDCs). However, how Clec4A4 controls immune responses through regulation of the function of CD8α(-) cDCs remains unclear. Here we show that Clec4A4 is a regulatory receptor for the activation of CD8α(-) cDCs that impairs inflammation and T-cell immunity. Clec4a4(-/-)CD8α(-) cDCs show enhanced cytokine production and T-cell priming following Toll-like receptor (TLR)-mediated activation. Furthermore, Clec4a4(-/-) mice exhibit TLR-mediated hyperinflammation. On antigenic immunization, Clec4a4(-/-) mice show not only augmented T-cell responses but also progressive autoimmune pathogenesis. Conversely, Clec4a4(-/-) mice exhibit resistance to microbial infection, accompanied by enhanced T-cell responses against microbes. Thus, our findings highlight roles of Clec4A4 in regulation of the function of CD8α(-) cDCs for control of the magnitude and quality of immune response.
Endothelial Nitric Oxide Synthase Gene Intron 4 (VNTR) Polymorphism and Vascular Access Graft Thrombosis (2007)

Vascular access thrombosis is a leading cause of vascular access failure in hemodialysis patients. Thrombosis is a multifactorial condition and genetic makeup can affect thrombosis risk. We conducted a study to investigate for possible associations between ecNOS gene intron 4 variable-number tandem repeat (VNTR) polymorphism and thrombosis of polytetrafluoroethylene hemodialysis arteriovenous access grafts (AVG) in Turkish patients. Fifty-five patients with end-stage renal disease who had AVGs implanted between 2000 and 2002 and 167 healthy individuals representing our healthy population were enrolled in this prospective study. Each subject provided a venous blood sample from which DNA was isolated, and polymerase chain reaction analysis was done to identify genotypes (aa, bb, ab) for ecNOS gene intron 4 VNTR polymorphism. All grafts were placed in brachioaxillary position. The subjects were divided into two groups based on duration of graft patency. The thrombosis group (Group I) comprised 26 patients who developed AVG thrombosis in the first 12 months after placement. The no-thrombosis group (Group II) comprised 29 patients whose grafts remained patient for at least 12 months. The frequency of the aa genotype in Group I was significantly higher than that in Group II (p = .005). At 6, 12, and 24 months, the primary patency rates for the AVGs in patients with the aa genotype were significantly lower than the corresponding rates for the bb and ab genotype groupings (p = .01, p = .01 and p = .04 for the three respective time points; Kaplan-Meier). ecNOS gene intron 4 VNTR polymorphism is linked with the pathogenesis of vascular access thrombosis in Turkish patients undergoing hemodialysis.
Ornithofauna of Efteni Lake In Düzce (2012)

Wetlands, many bird species sheltering, feeding and breeding are one of the important habitats. Anyinterference with wetlands directly affects the living things around here. In the past, which has 580 ha area EfteniLake, in the 1970s reduced up to 25 ha and the present work later in the year 1992 has reached 170 ha area. Thisstudy of bird species living in Lake Efteni determination is the first study. Total of 129 species were identified inthe study area. Maximum number of species have been identified in August. A total of 38 species produced inthe area where the IUCN criteria Aythya nyroca (Güldenstaedt, 1770) and Coracias garrulus L., 1758 nearthreated (NT), Aquila clanga Pall., 1811 vulnerable (VU), Oxyura leucocephala (Scop., 1769), endangered ( EN)was identified as a protected status.
Effects of synbiotic supplementation on insulin resistance in subjects with the metabolic syndrome: a randomised, double-blind, placebo-controlled pilot study (2014)

To evaluate the effects of synbiotic supplementation on insulin resistance and lipid profile in individuals with the metabolic syndrome, we conducted a randomised, double-blind, placebo-controlled pilot study on thirty-eight subjects with the metabolic syndrome; they were supplemented with either synbiotic capsules containing 200 million of seven strains of friendly bacteria plus fructo-oligosaccharide or placebo capsules twice a day for 28 weeks. Both the synbiotic (G1) and the placebo (G2) groups were advised to follow an energy-balanced diet and physical activity recommendations. Parameters related to the metabolic syndrome and insulin resistance were measured every 7 weeks during the course of the study. After 28 weeks of treatment, the levels of fasting blood sugar and insulin resistance improved significantly in the G1 group (P< 0·001). Both the G1 and G2 groups exhibited significant reductions in TAG levels ( - 71·22 v. - 10·47 mg/dl ( - 0·80 v. - 0·12 mmol/l) respectively; P< 0·001) and total cholesterol levels ( - 21·93 v. - 14·2 mg/dl ( - 0·57 v. - 0·37 mmol/l) respectively; P= 0·01), as well as increases in HDL levels (+7·7 v. +0·05 mg/dl (+0·20 v. +>0·01 mmol/l) respectively; P< 0·001). The mean differences observed were greater in the G1 group. No significant changes were observed in LDL levels, waist circumference, BMI, metabolic equivalent of task and energy intake between the groups. The present results indicate that synbiotic supplementation increases the efficacy of diet therapy in the management of the metabolic syndrome and insulin resistance.
Glucose Metabolism to Glucosamine Is Necessary for Glucose Stimulation of Transforming Growth Factor-α Gene Transcription (1996)

Transforming growth factor-alpha (TGFalpha) gene transcription can be increased when arterial smooth muscle cells are exposed to supraphysiological concentrations of glucose, and this effect of glucose can be mimicked by glucosamine. To determine whether the metabolism of glucose to glucosamine is required for this glucose effect, the rate-limiting step in glucose metabolism to glucosamine through the enzyme glutamine:fructose-6-phosphate amidotransferase (GFAT) was blocked using pharmacological and antisense strategies. We found that blockage of GFAT activity or expression significantly blunted the glucose-induced increase of TGFalpha expression. Blockage of GFAT also resulted in a decreased RL2 signal on intracellular proteins as detected by Western blotting and indirect immunofluorescence. The RL2 monoclonal antibody recognizes an epitope on proteins that contain N-acetylglucosamine and thus is a measure of protein glycosylation. Conversely, treatment of the cells with glucose and glucosamine resulted in an increase in the RL2 epitope on intracellular proteins. These results indicate that the metabolism of glucose to glucosamine is necessary for the transcriptional stimulation of TGFalpha expression in vascular smooth muscle cells by glucose. Furthermore, the level of glycosylation of some intracellular proteins can be modulated in response to physiological changes in the extracellular glucose concentration and the net activity of GFAT.
Assessment of skin temperature elevation and heat diffusion with electrosurgical currents. (1981)

A model was developed to quantitatively predict skin and tissue temperature elevations as a function of the amplitude and duration of the electrosurgical current applied, the geometry of the electrodes used, and the skin and tissue properties of the subjects. The temperature measurements were performed with thermographic equipment. The experimental data fit the model, thus fewer measurements will now be required for studies of other electrode designs.
Structure from linear or planar motions (1996)

We recently demonstrated a new approach to multiframe structure from motion from point features which, in the appropriate domain, provably reconstructs structure and motion correctly. The algorithm works for general motion and large perspective effects. In this paper, we describe how to adapt our approach to translational motion lying along a line or in a plane, with arbitrary rotations. An analysis of the bas-relief effect for multiple-motion sequences is also presented.
Stereochemistry of Diels-Alder Adducts. II. The Alkylation of Some Bicyclic Nitriles (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereochemistry of Diels-Alder Adducts. II. The Alkylation of Some Bicyclic NitrilesWerner R. Boehme, Edgar Schipper, William G. Scharpf, and Joseph NicholsCite this: J. Am. Chem. Soc. 1958, 80, 20, 5488–5495Publication Date (Print):October 1, 1958Publication History Published online1 May 2002Published inissue 1 October 1958https://pubs.acs.org/doi/10.1021/ja01553a044https://doi.org/10.1021/ja01553a044research-articleACS PublicationsRequest reuse permissionsArticle Views367Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improved parallel I/O via a two-phase run-time access strategy (1993)

As scientists expand their models to describe physical phenomena of increasingly large extent, I/O becomes crucial and a system with limited I/O capacity can severely constrain the performance of the entire program.We provide experimental results, performed on an lntel Touchtone Delta and nCUBE 2 I/O system, to show that the performance of existing parallel I/O systems can vary by several orders of magnitude as a function of the data access pattern of the parallel program. We then propose a two-phase access strategy, to be implemented in a runtime system, in which the data distribution on computational nodes is decoupled from storage distribution. Our experimental results show that performance improvements of several orders of magnitude over direct access based data distribution methods can be obtained, and that performance for most data access patterns can be improved to within a factor of 2 of the best performance. Further, the cost of redistribution is a very small fraction of the overall access cost.
Predictive monitoring for early detection of sepsis in neonatal ICU patients (2013)

Predictive monitoring has recently been shown to save lives. Harnessing and analyzing the vast amounts of physiologic data constantly displayed in ICU patients will lead to improved algorithms for early detection, prognosis, and therapy of critical illnesses.
<i>N</i>-{4-[3-(4-Fluorophenyl)pyrido[2,3-<i>b</i>]pyrazin-2-yl]-2-pyridyl}isopropylamine (2009)

In the crystal structure of the title compound, C(21)H(18)FN(5), the pyridopyrazine ring system forms dihedral angles of 33.27 (7) and 48.69 (9)° with the 4-fluoro-phenyl and pyridine ring, respectively. The dihedral angle of the 4-fluoro-phenyl and pyridine rings is 57.45 (8)°. The crystal packing is characterized by an inter-molecular N-H⋯N hydrogen bond.
Sodium Modeling During Hemodialysis: A New Approach (1984)

Sodium volume modeling during hemodialysis encounters several difficulties. First, the actual sodium distribution volume is the extracellular water, whereas the ultrafiltration flow reflects the variation of total body water. Thus, a two-pool model must be considered. This will complicate the model by increasing the number of parameters and boundary conditions. An alternative is to consider the total body water as the apparent distribution volume of loaded or removed sodium, which leads to a single-pool model. Second, convective sodium transfer induced by ultrafiltration is not negligible compared with diffusive sodium transfer. Therefore, sodium transfer modeling must simultaneously take into account the diffusive and the convective part, with the coupling part related to both processes. Third, the Donnan effect due to nondiffusible anionic plasma proteins modifies the sodium transfer through the membrane. Adequate sodium volume modeling should be a compromise between oversimplification, resulting in discrepancies between calculated values and experimental data, and overcomplexity, involving a great number of parameters and boundary conditions, which leads to a model unsuitable for clinical application. A single-pool model is proposed with only one parameter that is estimated during the first period of the hemodialysis session.
Tumor Targeting with Novel 6-Substituted Pyrrolo [2,3-<i>d</i>] Pyrimidine Antifolates with Heteroatom Bridge Substitutions via Cellular Uptake by Folate Receptor α and the Proton-Coupled Folate Transporter and Inhibition of de Novo Purine Nucleotide Biosynthesis (2016)

Targeted antifolates with heteroatom replacements of the carbon vicinal to the phenyl ring in 1 by N (4), O (8), or S (9), or with N-substituted formyl (5), acetyl (6), or trifluoroacetyl (7) moieties, were synthesized and tested for selective cellular uptake by folate receptor (FR) α and β or the proton-coupled folate transporter. Results show increased in vitro antiproliferative activity toward engineered Chinese hamster ovary cells expressing FRs by 4-9 over the CH2 analogue 1. Compounds 4-9 inhibited de novo purine biosynthesis and glycinamide ribonucleotide formyltransferase (GARFTase). X-ray crystal structures for 4 with FRα and GARFTase showed that the bound conformations of 4 required flexibility for attachment to both FRα and GARFTase. In mice bearing IGROV1 ovarian tumor xenografts, 4 was highly efficacious. Our results establish that heteroatom substitutions in the 3-atom bridge region of 6-substituted pyrrolo[2,3-d]pyrimidines related to 1 provide targeted antifolates that warrant further evaluation as anticancer agents.
Thermal Stability of Sulfonated Polymers (1995)

ABSTRACT Polyacrylamides which are used in oil applications i.e. polymer flooding and water based muds, are hydrolysed versus time and temperature. This leads to a lack of tolerance towards electrolyte contamination and to a rapid degradation inducing a loss of their properties. Modifications of polyacrylamide structure have been proposed to postpone their thermal stability to higher temperatures. Monomers such as acrylamido methylpropane sulfonate (AMPS) or sulfonated styrene/maleic anhydride can be used to prevent acrylamide comonomer from hydrolysis. The aim of this work is to study under controlled conditions, i.e. anaerobic atmosphere, neutral pH, the stability of sulfonated polymers in order to distinguish between hydrolysis and radical degradation reactions. It has been observed that up to 100°C, the AMPS group is stable and protects the acrylamide function from hydrolysis up to 80%. At higher temperature, even the hydrolysis of the AMPS group occurs, giving acrylate and β, β dimethyl taurine, with a kinetics that depends on temperature and time. Degradation in terms of molecular weight then occurs indicating that it follows a radical decarboxylation reaction. It can be limited either by the use of free radical scavenger or when the polymer is in the presence of a mineral phase such as bentonite. These results provide valuable data for the determination of the limits of use of sulfonated copolymers and guidelines for optimizing chemical structure of sulfonated polymers used in water based formulation, in particular to enhance their thermal stability.