gebhart/patrepeval huggingface dataset:
Tissue cultures and polyphenol production in Solidago altissima L. (2006)

Shoot, adventitious root and hairy root cultures of Solidago altissima L. were established. Chlorogenic acid and 3,5-dicaffeoylquinic acid were isolated from the hairy root cultures. 3,5-dicaffeoylquinic acid was the major secondary metabolites in various tissue cultures of this plant. These polyphenol compounds were easily isolated as the polyphenol-soybean protein complex from the extract of this plant. Solidago altissima L., producing polyphenol compounds with the high concentrations, seemed to have a potential to be as a new resource for functional compounds.
Study of first-order PMD for coherent optical orthogonal frequency division multiplexing system (2013)

Coherent optical orthogonal frequency division multiplexing system(CO-OFDM) is being considered as a promising technology for future high speed optical transmission with its resilience to fiber chromatic dispersion and ease of digital signal processing(DSP).Polarization Mode Dispersion(PMD) can influence system performance by bandwidth broadening and frequency-selective fading.With such a scheme,the mathematical model and effect of PMD to the transmission system is emphatically analyzed in CO-OFDM system based on first-order PDM(PMD-CO-OFDM).Analysis and simulation results show that,first-order PMD adds cosine factors for different OFDM subcarriers,which could be eliminated by channel equalization;meanwhile,PMD can reduce the fiber nonlinear penalty by decreasing the relativity of OFDM subcarriers.Without the consideration of chromatic dispersion,PMD-CO-OFDM system’s Q value is improved by about 0.2dB with differential group delay(DGD) 50ps compared with 30ps,for speed of 10Gbit/s transmission through standard-single-mode fiber(SSMF) and with 240km distance transmission.
Low Fermentable Oligo- Di- and Mono-Saccharides and Polyols (FODMAPs) or Gluten Free Diet: What Is Best for Irritable Bowel Syndrome? (2020)

Irritable Bowel Syndrome (IBS) is a very common functional gastrointestinal disease. Its pathogenesis is multifactorial and not yet clearly defined, and hence, its therapy mainly relies on symptomatic treatments. Changes in lifestyle and dietary behavior are usually the first step, but unfortunately, there is little high-quality scientific evidence regarding a dietary approach. This is due to the difficulty in setting up randomized double-blind controlled trials which objectively evaluate efficacy without the risk of a placebo effect. However, a Low Fermentable Oligo-, Di- and Mono-saccharides And Polyols (FODMAP) Diet (LFD) and Gluten Free Diet (GFD) are among the most frequently suggested diets. This paper aims to evaluate their possible role in IBS management. A GFD is less restrictive and easier to implement in everyday life and can be suggested for patients who clearly recognize gluten as a trigger of their symptoms. An LFD, being more restrictive and less easy to learn and to follow, needs the close supervision of a skilled nutritionist and should be reserved for patients who recognize that the trigger of their symptoms is not, or not only, gluten. Even if the evidence is of very low-quality for both diets, the LFD is the most effective among the dietary interventions suggested for treating IBS, and it is included in the most updated guidelines.
Phase transition of silver nanoparticles from aqueous solution to chloroform with the help of inclusion complexes of <i>p</i>-sulfonated calix[4]arene and alkanethiol molecules and its application in the size sorting of nanoparticles (2007)

Silver nanoparticles were synthesized in aqueous solution and capped with an inclusion complex of octadecanethiol (ODT) and p-sulfonated calix[4]arene (pSC4). By vigorous shaking of a biphasic mixture of the pSC4-threaded ODT-stabilized silver hydrosol and chloroform, the phase transition of the silver nanoparticles could be observed in the course of the transition of colour from the aqueous phase to the organic phase. The procedure of phase transition was independently verified by UV–vis spectroscopy, transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, and 1H nuclear magnetic resonance (NMR). In shaking the biphasic mixture, it was believed that pSC4 molecules were desorbed from the inclusion complex and the protective layer of silver nanoparticles shifted from hydrophilic to hydrophobic, which drove the phase transition of silver nanoparticles from aqueous solution into chloroform. More interestingly, the efficiency in the phase transition of aqueous silver nanoparticles rigidly depended on their sizes, which maybe used in the size sorting of nanoparticles.
Carbon-13 nuclear magnetic resonance spectra of hydroxy steroids (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-13 nuclear magnetic resonance spectra of hydroxy steroidsHanne Eggert, Craig L. VanAntwerp, Norman S. Bhacca, and Carl DjerassiCite this: J. Org. Chem. 1976, 41, 1, 71–78Publication Date (Print):January 1, 1976Publication History Published online1 May 2002Published inissue 1 January 1976https://doi.org/10.1021/jo00863a016RIGHTS & PERMISSIONSArticle Views349Altmetric-Citations210LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (1 MB) Get e-Alerts Get e-Alerts
Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation (2009)

Proteins are likely to organize into complexes that assemble and disassemble depending on cellular needs. When approximately 800 yeast strains expressing GFP-tagged proteins were grown to stationary phase, a surprising number of proteins involved in intermediary metabolism and stress response were observed to form punctate cytoplasmic foci. The formation of these discrete physical structures was confirmed by immunofluorescence and mass spectrometry of untagged proteins. The purine biosynthetic enzyme Ade4-GFP formed foci in the absence of adenine, and cycling between punctate and diffuse phenotypes could be controlled by adenine subtraction and addition. Similarly, glutamine synthetase (Gln1-GFP) foci cycled reversibly in the absence and presence of glucose. The structures were neither targeted for vacuolar or autophagosome degradation nor colocalized with P bodies or major organelles. Thus, upon nutrient depletion we observe widespread protein assemblies displaying nutrient-specific formation and dissolution.
Effects of genotype and genotype—environment interaction on deoxynivalenol accumulation and resistance to <i>Fusarium</i> head blight in rye, triticale, and wheat (2001)

Abstract Fusarium culmorum is one of the most important Fusarium species causing head blight infections in wheat, rye, and triticale. It is known as a potent mycotoxin producer with deoxynivalenol (DON), 3‐acetyl deoxynivalenol (3‐ADON), and nivalenol (NIV) being the most prevalent toxins. In this study, the effect of winter cereal species, host genotype, and environment on DON accumulation and Fusarium head blight (FHB) was analysed by inoculating 12 rye, eight wheat, and six triticale genotypes of different resistance levels with a DON‐producing isolate at three locations in 2 years (six environments). Seven resistance traits were assessed, including head blight rating and relative plot yield. In addition, ergosterol, DON and 3‐ADON contents in the grain were determined. A growth‐chamber experiment with an artificially synchronized flowering date was also conducted with a subset of two rye, wheat and triticale genotypes. Although rye genotypes were, on average, affected by Fusarium infections much the same as wheat genotypes, wheat accumulated twice as much DON as rye. Triticale was least affected and the grain contained slightly more DON than rye. In the growth‐chamber experiment, wheat and rye again showed similar head blight ratings, but rye had a somewhat lower relative head weight and a DON content nine times lower than wheat (3.9 vs. 35.3 mg/kg). Triticale was least susceptible with a five times lower DON content than wheat. Significant (P = 0.01) genotypic variation for DON accumulation existed in wheat and rye. The differences between and within cereal species in the field experiments were highly influenced by environment for resistance traits and mycotoxin contents. Nevertheless, mean mycotoxin content of the grain could not be associated with general weather conditions in the individual environments. Strong genotype‐environment interactions were found for all cereal species. This was mainly due to three wheat varieties and one rye genotype being environmentally extremely unstable. The more resistant entries, however, showed a higher environmental stability of FHB resistance and tolerance to DON accumulation. Correlations between resistance traits and DON content were high in wheat (P = 0.01), with the most resistant varieties also accumulating less DON, but with variability in rye. In conclusion, the medium to large genotypic variation in wheat and rye offers good possibilities for reducing DON content in the grains by resistance selection. Large confounding effects caused by the environment will require multiple locations and/or years to evaluate FHB resistance and mycotoxin accumulation.
Targeted Disruption of the Interaction between WD-40 Repeat Protein 5 (WDR5) and Mixed Lineage Leukemia (MLL)/SET1 Family Proteins Specifically Inhibits MLL1 and SETd1A Methyltransferase Complexes (2016)

MLL1 belongs to the SET1 family of histone H3 lysine 4 (H3K4) methyltransferases, composed of MLL1-4 and SETd1A/B. MLL1 translocations are present in acute leukemias, and mutations in several family members are associated with cancer and developmental disorders. MLL1 associates with a subcomplex containing WDR5, RbBP5, ASH2L, and DPY-30 (WRAD), forming the MLL1 core complex required for H3K4 mono- and dimethylation and transcriptional activation. Core complex assembly requires interaction of WDR5 with the MLL1 Win (WDR5 interaction) motif, which is conserved across the SET1 family. Agents that mimic the SET1 family Win motif inhibit the MLL1 core complex and have become an attractive approach for targeting MLL1 in cancers. Like MLL1, other SET1 family members interact with WRAD, but the roles of the Win motif in complex assembly and enzymatic activity remain unexplored. Here, we show that the Win motif is necessary for interaction of WDR5 with all members of the human SET1 family. Mutation of the Win motif-WDR5 interface severely disrupts assembly and activity of MLL1 and SETd1A complexes but only modestly disrupts MLL2/4 and SETd1B complexes without significantly altering enzymatic activity in vitro Notably, in the absence of WDR5, MLL3 interacts with RAD and shows enhanced activity. To further probe the role of the Win motif-WDR5 interaction, we designed a peptidomimetic that binds WDR5 (K<sub>d</sub> ∼3 nm) and selectively inhibits activity of MLL1 and SETd1A core complexes within the SET1 family. Our results reveal that SET1 family complexes with the weakest Win motif-WDR5 interaction are more susceptible to Win motif-based inhibitors.
Kerwin-Huelsman-Newcomb circuitusing current conveyors (1994)

Two equivalent circuits are generated using current conveyors to realise the same three transfer functions as in the Kerwin-Huelsman-Newcomb (KHN) biquad. Each circuit employs five current conveyors; two of them act as buffers. In both circuits, the two capacitors are grounded which is not the case in the KHN circuit.
Combining Gradual Deformation and Upscaling Techniques for Direct Conditioning of Fine-Scale Reservoir Models to Interference Test Data (2004)

Summary Reconciling multiscale data for reservoir characterization is a crucial issue, because different data types provide different information about the reservoir architecture and heterogeneity. It is essential that reservoir models preserve small-scale property variations observed in well logs and core measurements and capture the large-scale structure and continuity observed in global measures such as well-test and production data. In this paper, we present a new methodology to directly update fine-scale geostatistically-based reservoir models by combining gradual deformation parameterization for the fine-scale geostatistical model, and an upscaling technique for the coarse-scale flow simulation model. As consequence, both fine- and coarse-scale models are updated by dynamic data during the history matching process. The proposed methodology includes: Perturbation of the fine-scale geostatistical model using the gradual deformation parameterization. Gradual deformation ensures the preservation of the overall geostatistical properties of the fine model. Generation of the coarse-scale flow simulation model by upscaling the fine-scale geostatistical model. Fluid-flow simulation and sensitivity computation of the flow simulation results with respect to the fine-scale parameterization. This sensitivity computation is analytical and takes into account the upscaling process. Direct updating of the fine-scale geostatistical model using classical optimization process. Direct updating ensures consistency between the fine- and coarse-scale models. The accuracy of the proposed methodology was improved by calibrating the flow simulation model. The objective of this calibration is to reduce the error introduced by the upscaling step during the flow simulation. We applied successfully our methodology for fine-scale reservoir characterization process by integrating permanent downhole gauge measurements directly into a 3D-geostatistical model containing about two million gridblocks. This test is designed to highlight several key issues of the proposed methodology: Efficiency of the upscaling step, coupled with gradient-based optimization to speed up history matching. Usefulness of the calibration step for a correct integration of upscaling techniques in history matching. Capability of the methodology for maintaining consistency and coherency between fine-scale and coarse-scale models. Improvement of the reservoir characterization process by integrating dynamic data at both fine geostatistical scale and coarse simulation scale.
A convenient route to poly(methylphenylsilane)‐<i>graft</i>‐polystyrene copolymers (1997)

Abstract A graft copolymer of polystyrene on poly(methylphenylsilylene) was conveniently synthesised by using the bromomethyl groups of bromomethylated poly(methylphenylsilylene) as initiating sites for the bulk polymerisation of styrene by an atom transfer radical mechanism.
A review of current and future medical therapies for cerebral vasospasm following aneurysmal subarachnoid hemorrhage (2006)

In an effort to help clarify the current state of medical therapy for cerebral vasospasm, the authors reviewed the relevant literature on the established medical therapies used for cerebral vasospasm following aneurysmal subarachnoid hemorrhage (SAH), and they discuss burgeoning areas of investigation. Despite advances in the treatment of aneurysmal SAH, cerebral vasospasm remains a common complication and has been correlated with a 1.5- to threefold increase in death during the first 2 weeks after hemorrhage. A number of medical, pharmacological, and surgical therapies are currently in use or being investigated in an attempt to reverse cerebral vasospasm, but only a few have proven to be useful. Although much has been elucidated regarding its pathophysiology, the treatment of cerebral vasospasm remains a dilemma. Although a poor understanding of SAH-induced cerebral vasospasm pathophysiology has, to date, hampered the development of therapeutic interventions, current research efforts promise the eventual production of new medical therapies.
Hydropower development in remote locations of developing countries. [Cross-flow turbines] (1985)

In many developing countries hydropower can be used to replace the consumption of imported oil. The economic advantage of using hydropower increases if a low cost, locally manufactured turbine, called the cross flow turbine, can be used. This paper discusses the technical design and use of the cross flow turbine in the context of a hydroelectric development project in Africa. 12 references, 8 figures, 2 tables.
Flows in Stenotic Vessels (2000)

▪ Abstract The relationship between flow in the arteries, particularly the wall shear stresses, and the sites where atherosclerosis develops has motivated much of the research on arterial flow in recent decades. It is now well accepted that it is sites where shear stresses are low, or change rapidly in time or space, that are most vulnerable. These conditions are likely to prevail at places where the vessel is curved; bifurcates; has a junction, a side branch, or other sudden change in flow geometry; and when the flow is unsteady. These flows, often but not always involving flow separation or secondary motions, are also the most difficult ones in fluid mechanics to analyze or compute. In this article we review the modeling studies and experiments on steady and unsteady, two-and three-dimensional flows in arteries, and in arterial geometries most relevant in the context of atherosclerosis. These include studies of normal vessels—to identify, on the basis of the fluid mechanics, lesion foci—and stenotic vessels, to model and measure flow in vessels after the lesions have evolved into plaques sufficiently large to significantly modify the flow. We also discuss recent work that elucidates many of the pathways by which mechanical forces, primarily the wall shear stresses, are transduced to effect changes in the arterial wall at the cellular, subcellular, and genetic level.
On-line vehicle and pedestrian detections based on sign pattern (1994)

Real time detection methods of moving vehicles and pedestrians for navigation of the mobile robot are proposed. The method is based on a locomotion strategy, viz. signature-based stereotype motion. Signature of the moving vehicle is the shadow underneath the vehicle which is darker than any other parts of the asphalt paved road. Signature of the pedestrian is rhythm of walking. Rhythm of walking is unique to the pedestrian, and not influenced by time, weather, sunlight, shadow, and distance. Moreover, it is independent from clothes the pedestrian puts on. The result of experiments verify the validity of the methods.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Physical layout automation for system-on-packages (2004)

System-On-Package (SOP) technology provides a capability to integrate both mixed-signal active components and passive components all into a single high speed/density three dimensional packaging substrate. The physical layout resource of SOP is multi-layer in nature, where all layers are used for both placement and routing unlike the traditional multi-layer PCB or MCM packaging. In this paper, we present the first 3D physical design algorithms targeting SOP technology. 3D partitioning divides the input design into multiple layers. 3D placement determines the location of the active and passive components in multi-layer packaging substrate while considering various signal integrity issues. 3D global routing performs the following major steps: pin/net distribution, layer assignment, tree generation, and channel/pin assignment. Our experimental results demonstrate the effectiveness of our approaches.
Mass Measurements of Increased Accuracy Resolve Heterogeneous Populations of Intact Ribosomes (2006)

It is established that noncovalent complexes can be maintained both during and after electrospray and that assemblies of increasing size and complexity often lead to broadened peaks in mass spectra. This broadening arises from the tendency of large protein assemblies to form adducts with salts and is compounded when complexes are isolated directly from cells, without the full protein complement. To investigate the origins of this broadening in mass spectral peaks and to develop the optimal method for analyzing mass spectra of large protein complexes, we have carried out a systematic investigation of a series of noncovalent complexes representing a range of different sizes and architectures. We establish a positive correlation between peak width and the increased mass observed and show that this correlation is independent of the instrumental parameters employed. Using this relationship we show that we can determine masses of both 30S subunits and intact 2.3 MDa 70S ribosomes from Thermus thermophilus. The masses of both particles are consistent with multiple populations of ribosomes. To identify these various populations we combine simulated mass spectra of ribosomes, with and without the full protein complement, and estimate the extent of adducts from our study of known complexes. The results allow us to determine the contribution of the different subpopulations to the overall mass spectrum. We confirm the existence of these subpopulations using tandem mass spectrometry of intact 30S subunits. Overall, the results show that, rather than uniform particles, gas-phase ribosomes consist of a number of discrete populations. More generally, the results establish a rigorous procedure for accurate mass measurement and spectral analysis of heterogeneous macromolecular assemblies.
Adsorption-desorption Properties of Nitrogen Monoxide on Metal Ion-exchanged Zeolites (1992)

Abstract Among the samples tested, the amounts of reversible and irreversible adsorption of NO were the largest on copper ion-exchanged ZSM-5 zeolite and cobalt ion-exchanged ZSM-5 zeolite, respectively. In the former case, the amounts of reversible and irreversible adsorption of NO were dependent on the aluminum content of zeolite and linearly increased with increasing the exchange level.
The Graduate Curriculum (1984)

Article MetricsDownloadsCitationsNo data available.012AugSepOctNovDecJan510Total6 Months12 MonthsTotal number of downloads for the most recent 6 whole calendar months.
Direct chemiluminescence immunoassay for estradiol in serum. (1986)

In this simple, reliable, fast solid-phase chemiluminescence immunoassay for directly measuring (i.e., without prior extraction) estradiol-17 beta in serum, a monoclonal antibody is used that binds estradiol with high affinity (Ka = 10(10) L/mol), and does not bind other steroids tested, the highest cross reactivity observed being 0.1% for estradiol-17 alpha. In this system the monoclonal antibody is bound to the wells of microtiter plates via a second antibody directed against the monoclonal antibody. Fifty microliters of serum and estradiol-displacing agents are added, followed by 100 pg of estradiol-isoluminol conjugate, and the label is measured by luminometry after the binding reaction. The sensitivity of the assay is 180 pmol per liter of serum, and the effective working range at less than or equal to 10% CV is 270 to 6700 pmol/L. Analytical recovery of added estradiol averaged 99.7% (SD 6.5%). Within- and between-assay CVs ranged between 5 and 12.7%. Thirty-five unknown serum samples can be assayed within 4 h. Results correlated well with those obtained with a direct RIA: r = 0.94 (n = 149). This assay opens new perspectives for chemiluminescence immunoassays.