gebhart/patrepeval huggingface dataset:
Neuroprotective mechanisms of brain‐derived neurotrophic factor against 3‐nitropropionic acid toxicity: therapeutic implications for Huntington's disease (2010)

3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial succinate dehydrogenase that has been used to explore the molecular mechanisms of cell death associated with mitochondrial dysfunction and neurodegeneration for Huntington's disease (HD). Brain-derived neurotrophic factor (BDNF) is a neurotrophin that may regulate neuronal survival and differentiation. Experimental evidence derived from both clinical as well as basic research suggests a close association between BDNF deficiency and HD pathogenesis. In this review, we focus on recent progress in the molecular mechanisms responsible for the BDNF-mediated neuroprotective effects against mitochondrial dysfunction induced by 3-NP. Delineation of BDNF-mediated neuroprotective actions against 3-NP toxicity may add in the development of therapeutic intervention for HD where mitochondrial dysfunction is known to play a crucial role in pathogenesis of this devastating disease.
Nuclear NF-κB/p65 expression and response to neoadjuvant chemotherapy in breast cancer (2010)

Nuclear NF-κB expression is associated with ER negativity, higher Ki67 index and tumour grade. It was also found to be significantly associated with increased pCR but not clinical response to neoadjuvant chemotherapy.
A survey on vision-based fall detection (2015)

Falls are a major cause of fatal injury for the elderly population. To improve the quality of living for seniors, a wide range of monitoring systems with fall detection functionality have been proposed over recent years. This article is a survey of systems and algorithms which aim at automatically detecting cases where a human falls and may have been injured. Existing fall detection methods can be categorized as using sensors, or being exclusively vision-based. This literature review focuses on vision-based methods.
Click Chemistry: A Potential Platform for Development of Novel Dental Restorative Materials (2012)

The main aim of this study is to develop novel polymer modified glass-ionomer cement (GIC) glasses utilizing click chemistry and RAFT polymerization. These novel glasses can serve as a platform to improve the properties of GIC's by incorporating chemistries and formulations that are not normally compatible with GIC's. Aluminofluoro-silicate glasses utilized in glass-ionomer dental cements were coated with azide terminated silane groups. In addition, a copolymer of acrylic acid-itaconic acid containing alkyne groups was synthesized by RAFT polymerization and was coupled via ‘click’ chemistry with the azide-coated GIC glass particles. FT-IR spectroscopy was used to characterize the synthesized materials and to confirm completion of the ‘click’ coupling reaction. The experimental cements cured, demonstrating that these modified glasses could be utilized in GIC formulations. The long setting and working times compared to control groups indicate that further improvements are necessary to fully utilize this chemistry. Our initial results in this study demonstrated the successful application of click chemistry in developing novel dental restorative materials, specifically glass-ionomer cements.
Gene expression profiles identify a role for cyclooxygenase 2–dependent prostanoid generation in BMP6-induced angiogenic responses (2006)

The bone morphogenetic protein (BMP) family of proteins participates in regulation of angiogenesis in physiologic and pathologic conditions. To investigate the molecular mechanisms that contribute to BMP-dependent angiogenic signaling, we performed gene expression profiling of BMP6-treated mouse endothelial cells. We detected 77 mRNAs that were differentially regulated after BMP6 stimulation. Of these, cyclooxygenase 2 (Cox2) was among the most highly up-regulated by BMP stimulation, suggesting a role for Cox2 as a downstream regulator of BMP-induced angiogenesis. Up-regulation of Cox2 by BMP6 was detected at both mRNA and protein levels in endothelial cells, and BMP6 increased production of prostaglandins in a Cox2-dependent fashion. BMP6 up-regulated Cox2 at the transcriptional level through upstream SMAD-binding sites in the Cox2 promoter. Pharmacologic inhibition of Cox2, but not Cox1, blocked BMP6-induced endothelial cell proliferation, migration, and network assembly. BMP6-dependent microvessel outgrowth was markedly attenuated in aortic rings from Cox2-/- mice or after pharmacologic inhibition of Cox2 in aortas from wild-type mice. These results support a necessary role for Cox2 in mediating proangiogenic activities of BMP6. These data indicate that Cox2 may serve as a unifying component downstream from disparate pathways to modulate angiogenic responses in diseases in which neovascularization plays an underlying pathophysiologic role.
Helix α4 of the <i>Bacillus thuringiensis</i> Cry1Aa Toxin Plays a Critical Role in the Postbinding Steps of Pore Formation (2008)

Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in protein structure and formation of intermolecular disulfide bonds were investigated as possible factors responsible for the inactivity of these mutants. Incubation of each mutant with trypsin and chymotrypsin for 12 h did not reveal overt structural differences with Cry1Aa, although circular dichroism was slightly decreased in the 190- to 210-nm region for the I132C, S139C, and V150C mutants. The addition of dithiothreitol stimulated pore formation by the E128C, I132C, S139C, T142C, I145C, P146C, and V150C mutants. However, in the presence of these mutants, the membrane permeability never reached that measured for Cry1Aa, indicating that the formation of disulfide bridges could only partially explain their loss of activity. The ability of a number of inactive mutants to compete with wild-type Cry1Aa for pore formation in brush border membrane vesicles isolated from Manduca sexta was also investigated with an osmotic swelling assay. With the exception of the L147C mutant, all mutants tested could inhibit the formation of pores by Cry1Aa, indicating that they retained receptor binding ability. These results strongly suggest that helix alpha 4 is involved mainly in the postbinding steps of pore formation.
Error Analysis for Function Representation By the Sparse-Grid Combination Technique (1998)

Detailed error analyses are given for sparse-grid function representations through the combination technique. Two- and three-dimensional, and smooth and discontinuous functions are considered, as well as piecewise-constant and piecewise-linear interpolation techniques. Where appropriate, the results of the analyses are verified in numerical experiments. Instead of the common vertex-based function representation, cell-centered function representation is considered. Explicit, pointwise error expressions for the representation error are given, rather than order estimates. The paper contributes to the theory of sparse-grid techniques.
Integrating Dashcam Views through Inter-Video Mapping (2015)

In this paper, an inter-video mapping approach is proposed to integrate video footages from two dashcams installed on a preceding and its following vehicle to provide the illusion that the driver of the following vehicle can see-through the preceding one. The key challenge is to adapt the perspectives of the two videos based on a small number of common features since a large portion of the common region in the video captured by the following vehicle is occluded by the preceding one. Inspired by the observation that images with the most similar viewpoints yield dense and high-quality matches, the proposed inter-video mapping estimates spatially-varying motions across two videos utilizing images of very similar contents. Specifically, we estimate frame-to-frame motions of each two consecutive images and incrementally add new views into a merged representation. In this way, long-range motion estimation is achieved, and the observed perspective discrepancy between the two videos can be well approximated our motion estimation. Once the inter-video mapping is established, the correspondences can be updated incrementally, so the proposed method is suitable for on-line applications. Our experiments demonstrate the effectiveness of our approach on real-world challenging videos demonstrate the effectiveness of our approach.
Imaging White Light VISAR (1996)

An imaging white light velocimeter consisting of two imagine superimposing Michelson interferometers in series with the target interposed is demonstrated. Interferometrically measured 2-D velocity maps can be made of moving surfaces using unlimited bandwidth incoherent and extended area sources. Short pulse and broadband chirped pulse lasers can be used to provide temporal resolution not possible with monochromatic illumination. A 20 m/s per fringe imaging velociemter is demonstrated using an ordinary camera flash for illumination.
The Reed-Solomon encoders: Conventional versus Berlekamp's architecture (1982)

Concatenated coding was adopted for interplanetary space missions. Concatenated coding was employed with a convolutional inner code and a Reed-Solomon (RS) outer code for spacecraft telemetry. Conventional RS encoders are compared with those that incorporate two architectural features which approximately halve the number of multiplications of a set of fixed arguments by any RS codeword symbol. The fixed arguments and the RS symbols are taken from a nonbinary finite field. Each set of multiplications is bit-serially performed and completed during one (bit-serial) symbol shift. All firmware employed by conventional RS encoders is eliminated.
Natural localized molecular orbitals (1985)

The method of natural localized molecular orbitals (NLMOs) is presented as a novel and efficient technique for obtaining LMOs for SCF and CI wave functions. It is an extension of the previously developed natural atomic orbital (NAO) and natural bond orbital (NBO) methods, and uses only the information contained in the one-particle density matrix. Results are presented for methane and cytosine to indicate that NLMOs closely resemble LMOs obtained by the Boys and Edmiston–Ruedenberg methods, with the exception that the NLMO procedure automatically preserves the MO σ–π separation in planar molecules. The computation time is modest, generally only a small fraction of the SCF computation time. In addition, the derivation of NLMOs from NBOs gives direct insight into the nature of the LMO ‘‘delocalization tails,’’ thus enhancing the role of LMOs as a bridge between chemical intuition and molecular wave functions.
Evaluation of dynamic response of catheter-manometer systems for pulmonary arterial pressure (1994)

To quantitatively evaluate the waveform distortions observed through a catheter-manometer system, the tolerance range of amplitude errors and that of phase errors are defined as both within +/- 5%, and the frequency bandwidth was calculated where the frequency characteristics of amplitude and those of phase difference satisfy the two tolerance ranges by use of a second-order kinetic equation. The results were expressed by three variables, composed of the natural frequency (fn), damping coefficient (zeta), and highest frequency (fh) corresponding to the frequency bandwidth; a chart was constructed with fn on the x-axis, zeta on the y-axis, and fh as parameter. Also on the chart, the propagation delay times (td's) determined by fn, zeta, and fh were plotted. We measured the frequency characteristics of two 7-Fr Swan-Ganz catheters with lengths of 75 and 110 cm. fn's and zeta's were found to be 13.9 and 10.1 Hz and 0.23 and 0.32, respectively. Referring to this chart, the maximal fh these catheters would be able to reproduce within the tolerance ranges and the propagation td's can be predicted to be 3.2 and 2.4 Hz and 7 and 12 ms, respectively. Suppression of resonance by use of Accudynamic improved the maximal fh's to 3.9 and 2.9 Hz, respectively, but resulted in the increased td's to 14 and 19 ms due to increased zeta.
Profit Maximization over Social Networks (2012)

Influence maximization is the problem of finding a set of influential users in a social network such that the expected spread of influence under a certain propagation model is maximized. Much of the previous work has neglected the important distinction between social influence and actual product adoption. However, as recognized in the management science literature, an individual who gets influenced by social acquaintances may not necessarily adopt a product (or technology), due, e.g., to monetary concerns. In this work, we distinguish between influence and adoption by explicitly modeling the states of being influenced and of adopting a product. We extend the classical Linear Threshold (LT) model to incorporate prices and valuations, and factor them into users' decision-making process of adopting a product. We show that the expected profit function under our proposed model maintains submodularity under certain conditions, but no longer exhibits monotonicity, unlike the expected influence spread function. To maximize the expected profit under our extended LT model, we employ an unbudgeted greedy framework to propose three profit maximization algorithms. The results of our detailed experimental study on three real-world datasets demonstrate that of the three algorithms, PAGE, which assigns prices dynamically based on the profit potential of each candidate seed, has the best performance both in the expected profit achieved and in running time.
Influence of aromatic hydrocarbon receptor-mediated events on the genotoxicity of cigarette smoke condensate (1998)

The role of aromatic hydrocarbon receptor (AhR)-mediated events on the genotoxicity of mainstream cigarette smoke condensate was investigated. In vitro studies with mouse hepatoma cells stably transfected with a DRE-dependent luciferase reporter indicate that cigarette smoke condensate is able to transform AhR to an active form which is capable of initiating gene transcription. Micronucleus formation in two hepatoma cell lines was used as an index of genotoxicity. Cigarette smoke condensate was observed to induce a higher frequency of micronuclei in Hepa1c1c7 cells relative to TAOc1BP(r)c1 cells, which express approximately 10-fold less AhR. Furthermore, the frequency of micronuclei was potentiated when Hepa1c1c7 cells were pretreated with 2,3,7,8-tetrachlorodibenzo-p-dioxin, a high affinity ligand of AhR. These in vitro studies were followed by an in vivo experiment with Ahr+/+ and Ahr-/- mice. Animals were dosed for three consecutive days with cigarette smoke condensate (0.5-10 microg/kg/day, i.p. injection). The frequency of micronuclei in reticulocytes and total erythrocytes was determined in peripheral blood samples collected 24 h after the last administration. While condensate was found to increase the incidence of micronucleated reticulocytes in Ahr+/+ mice, no increase was observed in the null allele animals. Furthermore, the frequency of micronucleated erythrocytes, a measure of basal chromosome-damaging activity, was slightly but significantly higher in Ahr+/+ relative to Ahr-/- mice. Together, these data suggest that cigarette smoke contains chemicals which transform the AhR to an active transcription factor and AhR-regulated enzyme induction plays an important role in mediating the genotoxicity of this complex environmental pollutant.
Field trial of a staphylococcal mastitis vaccine in dairy herds: clinical, subclinical and microbiological assessments (1996)

The trial showed that the vaccine was efficacious in reducing the incidence of clinical mastitis and prevalence of subclinical mastitis in a herd that had a serious staphylococcal mastitis problem.
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in <i>Drosophila</i> (2014)

The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9 lines with ubiquitous or germ-line-restricted patterns of activity. We also demonstrate differential activity of the same gRNA expressed from different U6 snRNA promoters, with the previously untested U6:3 promoter giving the most potent effect. An appropriate combination of Cas9 and gRNA allows targeting of essential and nonessential genes with transmission rates ranging from 25-100%. We also demonstrate that our optimized CRISPR/Cas tools can be used for offset nicking-based mutagenesis. Furthermore, in combination with oligonucleotide or long double-stranded donor templates, our reagents allow precise genome editing by homology-directed repair with rates that make selection markers unnecessary. Last, we demonstrate a novel application of CRISPR/Cas-mediated technology in revealing loss-of-function phenotypes in somatic cells following efficient biallelic targeting by Cas9 expressed in a ubiquitous or tissue-restricted manner. Our CRISPR/Cas tools will facilitate the rapid evaluation of mutant phenotypes of specific genes and the precise modification of the genome with single-nucleotide precision. Our results also pave the way for high-throughput genetic screening with CRISPR/Cas.
IL-15 enhances the <i>in vivo</i> antitumor activity of tumor-reactive CD8 <sup>+</sup> T Cells (2004)

IL-15 and IL-2 possess similar properties, including the ability to induce T cell proliferation. However, whereas IL-2 can promote apoptosis and limit CD8(+) memory T cell survival and proliferation, IL-15 helps maintain a memory CD8(+) T cell population and can inhibit apoptosis. We sought to determine whether IL-15 could enhance the in vivo function of tumor/self-reactive CD8(+) T cells by using a T cell receptor transgenic mouse (pmel-1) whose CD8(+) T cells recognize an epitope derived from the self/melanoma antigen gp100. By removing endogenous IL-15 by using tumor-bearing IL-15 knockout hosts or supplementing IL-15 by means of exogenous administration, as a component of culture media or as a transgene expressed by adoptively transferred T cells, we demonstrate that IL-15 can improve the in vivo antitumor activity of adoptively transferred CD8(+) T cells. These results provide several avenues for improving adoptive immunotherapy of cancer in patients.
Synthesis and Amebicidal Activities of Some 1′,2′-Secoemetine Derivatives (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis and Amebicidal Activities of Some 1′,2′-Secoemetine DerivativesJ. P. Yardley, R. W. Rees, and Herchel. SmithCite this: J. Med. Chem. 1967, 10, 6, 1088–1091Publication Date (Print):November 1, 1967Publication History Published online1 May 2002Published inissue 1 November 1967https://pubs.acs.org/doi/10.1021/jm00318a023https://doi.org/10.1021/jm00318a023research-articleACS PublicationsRequest reuse permissionsArticle Views65Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evaluation of the Nuclisens HIV-1 QT Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma (1998)

Nuclisens HIV-1 QT is a new version of the NASBA HIV-1 QT assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma. The specificity of this assay was 100% in one laboratory and 99%-with nonrepeatability of the initial false positive-in another. The test was linear between 2.0 and 6.0 log RNA copies per ml. According to the input HIV-1 RNA concentration, accuracy varied from -0.11 to +0.10 log RNA copy per ml and precision varied from 0.66 to 0.14 log RNA copy per ml. Reproducibility decreased when the HIV-1 RNA level was near the lower limit of quantitation of the test. HIV-1 RNA could be quantitated by Nuclisens HIV-1 QT in 36% (laboratory 1) and 24% (laboratory 2) of clinical samples with HIV-1 RNA levels lower than the lower limit of quantitation by NASBA HIV-1 QT. Nuclisens HIV-1 QT was not suitable for measurement of RNA from clade G and group O HIV-1 strains.
Dualidad: competencias genéricas- dispositivos pedagógicos como estrategia para el aprendizaje en educación virtual (2013)

Este artículo, hace parte del proyecto “Diseño del modelo para la evaluación de las competencias: comunicativa, tecnológica e investigativa, en la modalidad de Educación Virtual de la Institución Universitaria Politécnico Grancolombiano”, en el cual se muestran algunos hallazgos que dan cuenta del papel dual que juegan las Competencias comunicativa, tecnológica e investigativa, denominadas Competencias genéricas, en la estructura de la propuesta virtual del Politécnico. Por una parte, el modelo virtual busca que estas competencias sean un aporte en la formación profesional y en la vida de los estudiantes y por otra, muestra cómo las mismas son dispositivos pedagógicos que le permiten a la propuesta virtual del Politécnico realizar su gestión y apropiación de conocimiento. El contenido del trabajo se dividirá en tres partes: en primera instancia, se precisarán algunos conceptos básicos para clarificar los temas abordados; en segundo lugar, se presentará la experiencia del Politécnico con las competencias y su papel dual como estrategia para el aprendizaje en Educación Virtual y, por último, se presentarán algunas conclusiones.