gebhart/patrepeval huggingface dataset:
Comparative Evaluation of First- and Second-Generation Digene Hybrid Capture Assays for Detection of Human Papillomaviruses Associated with High or Intermediate Risk for Cervical Cancer (1999)

In the present study we comparatively evaluated the first- and second-generation Digene Hybrid Capture assays for detection of human papillomaviruses (HPV) associated with high or intermediate risk for cervical cancer in cervical specimens. Concordant results were obtained with 468 of 483 (96.8%) specimens. All 15 specimens which gave repeatedly discordant results were positive by the second-generation test only, and 14 of them tested PCR positive. The enhanced sensitivity of the second-generation assay is mainly a result of the reformulation of hybridization reagents and, to a lesser extent, a result of the addition of new HPV probes.
Étude de la suspension active de siège (2005)

Cette thèse traite de l'intérêt de la suspension active de siège pour la réduction du niveau de vibrations transmises aux conducteurs de certains véhicules. Afin de garantir un coût modéré, un moteur électrique à aimant permanent est utilisé comme élément actif. Aussi, aucun élément passif tel qu'un élément de raideur ou encore un amortisseur visqueux n'est utilisé en parallèle de l'actionneur, ceci afin de réduire d'une part le nombre de composants constitutifs de la suspension, mais aussi l'incertitude sur le comportement dynamique de tels éléments, affectant directement la performance du système. Afin de minimiser la complexité associée au calcul de la loi de commande tout en gardant une performance satisfaisante, la théorie de la commande optimale linéaire quadratique est utilisée. On constate alors, à partir d'un modèle linéaire du comportement du système constitué de l'être humain couplé au siège, que la suspension active présente en théorie un gain de performance relativement modeste par rapport à une suspension passive dans un autobus urbain, tandis qu'elle est susceptible d'offrir une amélioration conséquente lorsque l'environnement vibratoire est celui d'un engin de terrassement de classe 1, tel que défini par la norme ISO 7096. Au niveau expérimental, deux prototypes de suspension active ont été évalués sur une plateforme reproduisant les vibrations d'un autobus urbain. Dans cet environnement vibratoire, une nette supériorité d'un des systèmes actifs sur une suspension passive commerciale a pu être observée. La performance des prototypes actifs a différé assez largement des prévisions théoriques, ceci étant largement dû au fait que les coussins de sièges expérimentaux avaient un comportement dynamique sensiblement différent de celui retenu dans l'étude théorique. L'influence de la nature du mécanisme de transmission sur la performance de la suspension active a pu aussi être étudiée.
A Grid-Connected Photovoltaic System with Direct Coupled Power Quality Control (2006)

This paper presents a grid-connected photovoltaic (PV) system with direct coupled power quality controller (PQC), which uses an inner current control loop (polarized ramp time (PRT)) and outer feedback control loops to improve grid power quality and maximum power point tracking (MPPT) of PV arrays. To reduce the complexity, cost and number of power conversions, which results in higher efficiency, a single stage CCVSI is used. The system operation has been divided into two modes (sunny and night). In night mode, the current controlled inverter (CCVSI) operates to compensate the reactive power demanded by nonlinear or variation in loads. In sunny mode, the proposed system performs PQC to reduce harmonic current and improve power factor as well as MPPT to supply active power from the PV arrays simultaneously. It is shown that the proposed system improves the system utilization factor (SUF) to 100%, which is generally low for PV systems (20%). Mathematical modelling, computer simulations and experimental results for a 1 kVA CCVSI are presented
Classified security protection evaluation for vehicle information system (2015)

This paper firstly analyzes security vulnerabilities, threats and special security requirements of current vehicle information system. And further referring to the classified security protection evaluation standards of traditional information system, we establish classified security protection evaluation system for vehicle information system. This system summarizes typical information assets in car system, divides vehicle information system into two classes/levels: family and business, and defines target and requirement of security protection for two kinds of vehicle information system, respectively. Finally, a series of feasible evaluation methods and tools are presented for evaluation practice. A big contribution of this paper is to explore classified security protection evaluation for vehicle information system and fills up the gap of evaluating security state of automotive information system all over the world.
Beziehungen zwischen Struktur und Reaktivität ambifunktioneller nucleophiler Verbindungen (1964)

Theoretische Überlegungen und neue präparative Ergebnisse haben in den letzten Jahren die Kenntnisse von der Struktur und Reaktivität ambifunktioneller nucleophiler Verbindungen vertieft und erweitert. Es wird versucht, einen Überblick über die Theorien zu geben, die zur Deutung des Verhaltens dieser Verbindungen gegenüber elektrophilen Agentien entwickelt worden sind, und Regeln aufzustellen, die als Leitfaden für die Durchführung neuer Reaktionen dienen können.
Induction of Autophagy Promotes Fusion of Multivesicular Bodies with Autophagic Vacuoles in K562 Cells (2007)

Morphological and biochemical studies have shown that autophagosomes fuse with endosomes forming the so-called amphisomes, a prelysosomal hybrid organelle. In the present report, we have analyzed this process in K562 cells, an erythroleukemic cell line that generates multivesicular bodies (MVBs) and releases the internal vesicles known as exosomes into the extracellular medium. We have previously shown that in K562 cells, Rab11 decorates MVBs. Therefore, to study at the molecular level the interaction of MVBs with the autophagic pathway, we have examined by confocal microscopy the fate of MVBs in cells overexpressing green fluorescent protein (GFP)-Rab11 and the autophagosomal protein red fluorescent protein-light chain 3 (LC3). Autophagy inducers such as starvation or rapamycin caused an enlargement of the vacuoles decorated with GFP-Rab11 and a remarkable colocalization with LC3. This convergence was abrogated by a Rab11 dominant negative mutant, indicating that a functional Rab11 is involved in the interaction between MVBs and the autophagic pathway. Interestingly, we presented evidence that autophagy induction caused calcium accumulation in autophagic compartments. Furthermore, the convergence between the endosomal and the autophagic pathways was attenuated by the Ca2+ chelator acetoxymethyl ester (AM) of the calcium chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), indicating that fusion of MVBs with the autophagosome compartment is a calcium-dependent event. In addition, autophagy induction or overexpression of LC3 inhibited exosome release, suggesting that under conditions that stimulates autophagy, MVBs are directed to the autophagic pathway with consequent inhibition in exosome release.
A proteoglycan that activates fibroblast growth factors during early neuronal development is a perlecan variant (1996)

Cells in the early embryonic vertebrate nervous system are dependent on members of the fibroblast growth factor (FGF) family for their proliferation and subsequent differentiation. These growth factors will only bind to their specific high affinity cell surface receptors after formation of a ternary complex with the glycosaminoglycan heparan sulfate. Such specific heparan sulfates are secreted as proteoglycans from neural precursor cells and localise to their surfaces. One such proteoglycan, HSPG-PRM (Perlecan-related molecule), was isolated through its ability to potentiate neural cell responses to either FGF-1 or FGF-2. In this study, we have verified the relative molecular mass of the core protein of PRM as 45,000 and obtained partial amino acid sequence from it. The sequences bore significant homology to native perlecan. A probe generated by reverse transcriptase polymerase chain reaction using oligonucleotides designed from the protein sequence used on northern blots of RNA from a neuroepithelial cell line detected perlecan at 12.6 kilobases, as well as novel transcripts at 6.5 and 3.5 kilobases. The latter species appears by virtue of its size and abundance to be the novel PRM transcript. PRM appears to be encoded by the same gene as perlecan, as genomic Southern blotting only detected a single gene. Polyclonal antibodies raised against the PRM molecule detected a single proteoglycan species at 290x10(3) with a core protein of 45x10(3). Polyclonal anti-perlecan antibodies cross-reacted with PRM confirming their relatedness, although immunohistochemical studies revealed a differential staining pattern for PRM as compared to perlecan within the developing nervous system. The PRM molecule was shown to be localised to several different tissues of the developing embryo, indicating that it plays a broad role. We conclude that PRM is a variant of perlecan that is differentially glycosylated in a manner that confers highly specific functions at critical stages of neural development and tissue growth.
Evaluation of Marine Alga Ulva lactuca L. as A Source of Natural Preservative Ingredient (2008)

Total chlorophyll (T-Chl), total carotenoids (TCAR) and total phenolics (TPc) compounds were quantified in the biomasses of Ulva latutus grown either in normal (NSW) or artificial sea water (ASW) under indoor condition. The Ulva crude organic extracts (Ulva-COEs) analyzed by chromatographic method. Antioxidant and antibacterial activities of these extracts were determined. Seven and thirty-four compounds characterized in both Ulva-COEs by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Chl a (15.60-30.90%), Chl b (12.20-14.89%), 9-cis β-carotene (13.12- 14.47%), β-carotene (11.44-11.47%) and all-trans β-carotene (6.16-29.70%, of the total area) were recognized as major compounds. The UCOEs exhibited remarkable antioxidant activity, compared to a synthetic antioxidant: butylated hydroxyanisol (BHA) and butylated hydroxyltoluene (BHT), as evaluated by the rapid TLC screening, β-carotene bleaching, 2,2-diphenyl-1-picrylhydrazyl (DPPH . ) radical scavenging methods. The positively correlation was apparent between the antioxidant activity (AOE) and present of carotenoids, phenolics and chlorophylls in their extracts. In addition Ulva-COEs exhibited great potential antibacterial activities against six bacterial strains, with minimal inhibitory concentration (MIC) values ranged from 400 to 350 µg/ ml. Data indicate that U. latutus could be considered as potential rich source of natural colorant with antioxidant and antibacterial properties and could be utilized in as natural preservative ingredient in food and in pharmaceutical industry.
Weekly docetaxel in patients with recurrent and/or metastatic squamous cell carcinoma of the head and neck (2005)

The results of this study suggested that weekly docetaxel was an active agent for patients with recurrent and/or metastatic squamous cell carcinoma of the head and neck.
Synthetic ligands for PreQ1 riboswitches provide structural and mechanistic insights into targeting RNA tertiary structure (2019)

Riboswitches are naturally occurring RNA aptamers that regulate gene expression by binding to specific small molecules. Riboswitches control the expression of essential bacterial genes and are important models for RNA-small molecule recognition. Here, we report the discovery of a class of synthetic small molecules that bind to PreQ<sub>1</sub> riboswitch aptamers. These molecules bind specifically and reversibly to the aptamers with high affinity and induce a conformational change. Furthermore, the ligands modulate riboswitch activity through transcriptional termination despite no obvious chemical similarity to the cognate ligand. X-ray crystallographic studies reveal that the ligands share a binding site with the cognate ligand but make different contacts. Finally, alteration of the chemical structure of the ligand causes changes in the mode of RNA binding and affects regulatory function. Thus, target- and structure-based approaches can be used to identify and understand the mechanism of synthetic ligands that bind to and regulate complex, folded RNAs.
Simultaneous measurement of chromatin accessibility, DNA methylation, and nucleosome phasing in single cells (2016)

Abstract Gaining insights into the regulatory mechanisms that underlie the transcriptional variation observed between individual cells necessitates the development of methods that measure chromatin organization in single cells. Here I adapted N ucleosome O ccupancy and Me thylome-sequencing (NOMe-seq) to measure chromatin accessibility and endogenous DNA methylation in single cells (scNOMe-seq). scNOMe-seq recovered characteristic accessibility and DNA methylation patterns at DNase hypersensitive sites (DHSs). An advantage of scNOMe-seq is that sequencing reads are sampled independently of the accessibility measurement. scNOMe-seq therefore controlled for fragment loss, which enabled direct estimation of the fraction of accessible DHSs within individual cells. In addition, scNOMe-seq provided high resolution of chromatin accessibility within individual loci which was exploited to detect footprints of CTCF binding events and to estimate the average nucleosome phasing distances in single cells. scNOMe-seq is therefore well-suited to characterize the chromatin organization of single cells in heterogeneous cellular mixtures.
The effects of Provent on moderate to severe obstructive sleep apnoea during continuous positive airway pressure therapy withdrawal: a randomised controlled trial (2013)

NCT01332175.
Nanoelectronic logic circuits with carbon nanotube transistors (2007)

We demonstrate logic circuits with carbon nanotube field-effect transistors (CNTFETs). The p-type CNTFETs are fabricated based on single-walled carbon nanotubes and n-type CNTFETs are fabricated based on nitrogen-doped multiwalled carbon nanotubes. To be specitic, we demonstrate three types of logic circuits with CNTFETs, which include an inverter (a p-type CNTFET), a complementary inverter (a p-type CNTFET and an n-type CNTFET in a single clip), and a logic NOR (two p-type CNTFETs).
Novel protecting group for the synthesis of 7.alpha.-D-pentofuranosylhypoxanthines (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel protecting group for the synthesis of 7.alpha.-D-pentofuranosylhypoxanthinesYoshihisa Mizuno, Wongil Limn, Kiyomi Tsuchida, and Kazuyoshi IkedaCite this: J. Org. Chem. 1972, 37, 1, 39–42Publication Date (Print):January 1, 1972Publication History Published online1 May 2002Published inissue 1 January 1972https://pubs.acs.org/doi/10.1021/jo00966a010https://doi.org/10.1021/jo00966a010research-articleACS PublicationsRequest reuse permissionsArticle Views100Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Nongenomic Activity of Ligands in the Association of Androgen Receptor with Src (2007)

Androgen receptor (AR) induces cell proliferation by increasing the kinase activity of Src. We describe an approach for discriminating agonist and antagonist in a nongenomic steroid-signaling pathway using an association of AR with Src. We constructed a pair of genetically encoded indicators, where N- and C-terminal fragments of split firefly luciferase (FLuc) were fused to AR and Src, respectively. The fusion proteins with AR and Src are localized in the cytoplasm and on the plasma membrane, respectively. Upon being activated with androgen, AR undergoes an intramolecular conformational change and binds with Src. The association causes the complementation of the split FLuc and recovery of FLuc activity. The resulting luminescence intensities were taken as a measure of the rapid hormonal activity of steroids in the nongenomic AR signaling. Ten minutes are required for the AR-Src association by 5alpha-dihydroxytestosterone (DHT), which was completely inhibited by an antagonist, cyproterone acetate. The activities of ligands in the nongenomic pathway of AR were compared with those in the genomic pathway obtained on the basis of the nuclear trafficking of AR in mammalian cells. The comparison revealed that DHT and testosterone activate both genomic and nongenomic pathways of AR. 17beta-Estradiol and progesterone were found to be specific activators only for the genomic signaling pathway of AR. On the other hand, procymidone exhibited a specific activity only for the nongenomic signaling pathway of AR. The present approach is the first example addressing the agonistic and antagonistic activities of ligands in a nongenomic pathway of AR.
Imaging by integrating stitched spectrograms (2013)

A new diffractive imaging technique called Imaging By Integrating Stitched Spectrograms (IBISS) is presented. Both the data collection and phase retrieval algorithm used in IBISS are direct extensions of frequency resolved optical gating to higher dimensions. Data collection involves capturing an array of diffraction patterns generated by scanning a sample across a coherent beam of light. Phase retrieval is performed using the Principal Components Generalized Projections Algorithm (PCGPA) by reducing the four dimensional data set of images to two remapped two-dimensional sets. The technique is successfully demonstrated using a Helium Neon laser to image a 350μm wide sample with 12μm resolution.
A simple nonradioactive method for the determination of the binding affinities of antibodies induced by hapten bioconjugates for drugs of abuse (2015)

The accurate analytical measurement of binding affinities of polyclonal antibody in sera to heroin, 6-acetylmorphine (6-AM), and morphine has been a challenging task. A simple nonradioactive method that uses deuterium-labeled drug tracers and equilibrium dialysis (ED) combined with ultra performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) to measure the apparent dissociation constant (K d) of antibodies to 6-AM and morphine is described. The method can readily detect antibodies with K d in the low nanomolar range. Since heroin is rapidly degraded in sera, esterase inhibitors were included in the assay, greatly reducing heroin hydrolysis. MS/MS detection directly measured the heroin in the assay after overnight ED, thereby allowing the quantitation of % bound heroin in lieu of K d as an alternative measurement to assess heroin binding to polyclonal antibody sera. This is the first report that utilizes a solution-based assay to quantify heroin-antibody binding without being confounded by the presence of 6-AM and morphine and to measure K d of polyclonal antibody to 6-AM. Hapten surrogates 6-AcMorHap, 6-PrOxyHap, MorHap, DiAmHap, and DiPrOxyHap coupled to tetanus toxoid (TT) were used to generate high affinity antibodies to heroin, 6-AM, and morphine. In comparison to competition ED-UPLC/MS/MS which gave K d values in the nanomolar range, the commonly used competition enzyme-linked immunosorbent assay (ELISA) measured the 50% inhibition concentration (IC50) values in the micromolar range. Despite the differences in K d and IC50 values, similar trends in affinities of hapten antibodies to heroin, 6-AM, and morphine were observed by both methods. Competition ED-UPLC/MS/MS revealed that among the five TT-hapten bioconjugates, TT-6-AcMorHap and TT-6-PrOxyHap induced antibodies that bound heroin, 6-AM, and morphine. In contrast, TT-MorHap induced antibodies that poorly bound heroin, while TT-DiAmHap and TT-DiPrOxyHap induced antibodies either did not bind or poorly bound to heroin, 6-AM, and morphine. This simple and nonradioactive method can be extended to other platforms, such as oxycodone, cocaine, nicotine, and methamphetamine for the selection of the lead hapten design during substance abuse vaccine development.
Improved Performance of Passive-Matrix Micro-LED Displays Using a Multi-Function Passivation Structure (2020)

The enhanced light output efficiency of passive-matrix micro light-emitting-diode (LED) display was achieved by employing the tapered sidewalls and distributed-Bragg-reflector (DBR) passivation structure. The oblique angles at the bottom of multi-quantum wall and n-GaN are 48.9° and 21.8°, which can favor the planarization structure of pixel interconnection. As increasing the number of DBR (SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /TiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) stacking from 3.5 to 5.5 pairs, the reflectance increased obviously from 81.4% to 98.0% at the wavelength of 450 nm. As the micro-LED display with a multi-function DBR structure, the output power of 1.07 mW under 1 mA, the external-quantum efficiency of 42% under 0.2 mA, and the luminance of 435 nits were obtained.
Profiling early infection responses: <i>Pseudomonas aeruginosa</i> eludes host defenses by suppressing antimicrobial peptide gene expression (2005)

Insights into the host factors and mechanisms mediating the primary host responses after pathogen presentation remain limited, due in part to the complexity and genetic intractability of host systems. Here, we employ the model Drosophila melanogaster to dissect and identify early host responses that function in the initiation and progression of Pseudomonas aeruginosa pathogenesis. First, we use immune potentiation and genetic studies to demonstrate that flies mount a heightened defense against the highly virulent P. aeruginosa strain PA14 when first inoculated with strain CF5, which is avirulent in flies; this effect is mediated via the Imd and Toll signaling pathways. Second, we use whole-genome expression profiling to assess and compare the Drosophila early defense responses triggered by the PA14 vs. CF5 strains to identify genes whose expression patterns are different in susceptible vs. resistant host-pathogen interactions, respectively. Our results identify pathogenesis- and defense-specific genes and uncover a previously undescribed mechanism used by P. aeruginosa in the initial stages of its host interaction: suppression of Drosophila defense responses by limiting antimicrobial peptide gene expression. These results provide insights into the genetic factors that mediate or restrict pathogenesis during the early stages of the bacterial-host interaction to advance our understanding of P. aeruginosa-human infections.