gebhart/patrepeval huggingface dataset:
Differential sensitivity of two functions of the insulin receptor to the associated proteolysis: kinase action and hormone binding. (1986)

Since we observed that after purification the receptor kinase activity is rapidly lost under conditions where insulin binding function seems to be preserved, we have studied the cause(s) of receptor kinase inactivation. Highly purified placental insulin receptor preparations were analyzed by NaDodSO4/PAGE followed by silver staining or immunostaining using domain-specific antibodies raised against synthetic peptides corresponding to the amino acid sequences of the beta subunit. These studies revealed the intact 90-kDa beta subunit is degraded first to an 88-kDa form and then to a 50-kDa beta 1-subunit form by proteolysis even after purification when stored at 4 degrees C. The 88-kDa beta subunit, which lacks the carboxyl-terminal approximately equal to 2-kDa portion exhibits almost no autophosphorylation activity, nor does insulin stimulate autophosphorylation. The loss of kinase activity as measured by phosphorylation of the src-related peptide is correlated with the loss of the intact 90-kDa beta subunit. Degradation of the beta subunit to the 50-kDa form seems to be facilitated by the removal of the approximately equal to 2-kDa peptide. Present studies thus suggest that only the intact form of the beta subunit has full kinase activity in an insulin-dependent manner and that other forms, such as the 88-kDa beta subunit show little kinase activity. The inactivation appears to arise from a conformational change of the 90-kDa form, which makes it susceptible to proteolysis at the carboxyl-terminal end. These results imply that the carboxyl-terminal of the beta subunit is important for the manifestation of the tyrosine kinase activity of the insulin receptor.
CLOUDTHINK: A SCALABLE SECURE PLATFORM FOR MIRRORING TRANSPORTATION SYSTEMS IN THE CLOUD (2015)

We present a novel approach to developing a vehicle communication platform consisting of a low-cost, open-source hardware for moving vehicle data to a secure server, a Web Application Programming Interface (API) for the provision of third-party services, and an intuitive user dashboard for access control and service distribution. The CloudThink infrastructure promotes the commoditization of vehicle telematics data by facilitating easier, flexible, and more secure access. It enables drivers to confidently share their vehicle information across multiple applications to improve the transportation experience for all stakeholders, as well as to potentially monetize their data. The foundations for an application ecosystem have been developed which, taken together with the fair value for driving data and low barriers to entry, will drive adoption of CloudThink as the standard method for projecting physical vehicles into the cloud. The application space initially consists of a few fundamental and important applications (vehicle tethering and remote diagnostics, road-safety monitoring, and fuel economy analysis) but as CloudThink begins to gain widespread adoption, the multiplexing of applications on the same data structure and set will accelerate its adoption.
Silos theory and practice (1976)

Diagrs., graphs, illus., tables. Bibliography p. 251. Translated from French; Distributed by: Trans Tech S.A. CH-4711 Aedermannsdorf, Switzerland
Review of Hydrogen Isotope Permeability Through Materials (1983)

This report is the first part of a comprehensive summary of the literature on hydrogen isotope permeability through materials that do not readily form hydrides. While we mainly focus on pure metals with low permeabilities because of their importance to tritium containment, we also give data on higher-permeability materials such as iron, nickel, steels, and glasses.
A High-Frequency Resonant Converter Based on the Class Phi2 Inverter for Wireless Power Transfer (2014)

This paper presents the design and implementation of high frequency resonant converter based on the Class Φ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> inverter for inductive power transfer. MHz frequency operation can allow for higher power density than conventional switching frequencies. The converter is based on the Class Φ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> inverter, reducing the voltage stress across the switch compared to other resonant topologies. A prototype of converter and a few cm airgap inductive coupling system with resistive load was implemented. We will demonstrate a greater than hundred Watt push-pull resonant converter for inductive power transfer at 13.56MHz.
OO design and implementation: Java and Java analysis studio (2000)

We present an overview of the Java language, Java virtual machine, and the large set of standard libraries and extensions available for Java, and provide a discussion of issues that effect Java performance.This is followed by an introduction to Java Analysis Studio (JAS), a tool written in Java, and in which Java is the language used to perform data analysis.We will also explore how OO techniques have been used to built a system from modular components including visualization, fitting and data-access components that can be used together, or on their own. JAVA
On‐line gas analysis in animal cell cultivation: II. Methods for oxygen uptake rate estimation and its application to controlled feeding of glutamine (1995)

Different methods for oxygen uptake rate (OUR) determinations in animal cell cultivation were investigated using a high quality mass spectrometer. Dynamic measurements have considerable disadvantages because of disturbances of the growing cells by the necessary variations of dissolved oxygen concentration. Only infrequent discrete measurements are possible using this method. Stationary liquid phase balance yielded better results with much higher frequency. Gas phase balancing has the advantage of not requiring dissolved oxygen measurement and knowledge of K(L)a, both of them are easily biased. It was found that simple gas phase balancing is either very inaccurate (error larger than expected signal) or very slow, with gas phase residence times of several hours. Therefore, a new method of aeration was designed. Oxygen and CO(2) transfer are mainly achieved via sparging. The gas released to the headspace is diluted with a roughly 100-fold stream of an inert gas (helium). Through this dilution, gas ratios are not changed for O(2), CO(2), Ar, and N(2). The measurement of lower concentrations (parts per million and below) is easy using mass spectrometry with a secondary electron multiplier. With this new method an excellent accuracy and sufficient speed of analysis were obtained. All these on-line methods for OUR measurement were tested during the cultivation of animal cells. The new method allowed better study of the kinetics of animal cell cultures as was shown with a hybridoma cell line (HFN 7.1, ATCC CRL 1606) producing monoclonal antibodies against human fibronectin. With the aid of these methods it was possible to find a correlation between a rapid decrease in oxygen uptake rate (OUR) and glutamine concentration. The sudden decrease in OUR can be attributed to glutamine depletion. This provided a basis for the controlled addition of glutamine to reduce the formation of ammonia produced by hydrolysis. This control method based on OUR measurement resulted in increased cell concentration and threefold higher product concentration. (c) 1995 John Wiley & Sons, Inc.
Application of blockchain in integrated energy system transactions (2020)

Blockchain technology is the underlying technology of Bitcoin, which is fair, transparent and decentralized. The integrated energy system has the characteristics of open interconnection, user-centered and distributed peer-to-peer sharing, and its energy trading model will also be developed centrally to distributed. The characteristics of blockchain technology make it naturally applicable to energy transactions in integrated energy systems. This article first analyzes the characteristics of the integrated energy system market and summarizes the participants in the market. Then, based on the existing research and analysis, a blockchain-based energy transaction architecture is designed, and a weakly centralized management method is introduced. finally, the problems and challenges faced by the application of blockchain in energy transactions are analyzed.
Mechanical Stability of Ultra Thin Ge/Si Film on SiO$_2$:the Effect of Si/SiO$_2$ Interface (2005)

This material is presented to ensure timely dissemination of scholarly and technical work. Copyright and all rights therein are retained by authors or by other copyright holders. All persons copying this information are expected to adhere to the terms and constraints invoked by each author's copyright. In most cases, these works may not be reposted without the explicit permission of the copyright holder.
Eleven Pipers Piping ♪ ♪ ♪ (1985)

The DOS‐tips column in the September 1985 issue of OCLC Micro addressed some common uses of the DIRectory command in DOS. While training OCLC staff members on the IBM PC, I have found some other handy variations of DIR, including some of the piping and redirection variations. This article lists 19 DIR command variations that I think are probably all the ones you are most likely to use.
[Quantitative competitive RT-PCR and quantitative detection of Escheriia coli acrA-mRNA]. (2007)

A Quantitative detection assay of acrA-mRNA of Escherichia coli ATCC25922 was developed by Quantitative Competitive RT-PCR. Target Standard(TS) which was same as target-templete acrA was amplified by PCR with P1 and P2 as primers. Internal Standard (IS) which was shorter 68 bp then target-templete acrA was amplified by temperature-gradient-PCR with P1 and P3P2 as primers, whose annealing temperatures ranged from 55-65 degrees C, and the most suitable annealing temperature was acquired at 56 degrees C. Both TS and IS were largely amplified by PCR as above and extracted to store. Co-amplification with both TS and IS as templetes was optimized by temperature-gradient-PCR with P1 and P2 as primers, whose annealing temperatures were ranged from 55-65 degrees C, and then the most suitable annealing temperature was also acquired at 56 degrees C. Then co-amplification optimized as above was did again but with both cDNA of Escherichia coli (with target-templete acrA-cDNA copies unknown) and IS (10-fold serial dilution, and with IS copies known) as templates. The electrophoresis bands were photographed and analysed with UVI band and each band area was acquired, then linear regression analysis was did with SPSS ll.5 and CurveExpert l.3 and a competitive curve was drawn as y = -0.345 + 0.097x. Results revealed that the two kinds of product electrophoresis bands of co-amplification, whose templates were both 10-fold diluted IS and cDNA, could be distinguished clearly in 1.5% agarose gel because of 68bp discrepancy, and showed lighteness dimming gradually with IS copies 10-fold diluting. With the competitive curve, the copies of acrA-mRNA in sample could be counted accurately and easily.
Chemistry of the Metal Carbonyls. IV. Reactions between Certain Metal Carbonyls and Some Condensed Polycyclic Organic Systems<sup>1,2</sup> (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of the Metal Carbonyls. IV. Reactions between Certain Metal Carbonyls and Some Condensed Polycyclic Organic Systems1,2R. B. King and F. G. A. StoneCite this: J. Am. Chem. Soc. 1960, 82, 17, 4557–4562Publication Date (Print):September 1, 1960Publication History Published online1 May 2002Published inissue 1 September 1960https://pubs.acs.org/doi/10.1021/ja01502a028https://doi.org/10.1021/ja01502a028research-articleACS PublicationsRequest reuse permissionsArticle Views458Altmetric-Citations72LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The changing epidemiology of resistance (2009)

Antibiotic resistance is now a linked global problem. Dispersion of successful clones of multidrug resistant (MDR) bacteria is common, often via the movement of people. Local evolution of MDR bacteria is also important under the pressure of excessive antibiotic use, with horizontal gene transfer providing the means by which genes such as bla(CTX-M) spread amongst different bacterial species and strains. Beta-lactamase production is a common resistance mechanism in Gram-negative bacteria, and the rapid dissemination of novel genes reflects their evolution under the selective pressure of antibiotic usage. Many Enterobacteriaceae now carry broad-spectrum beta-lactamases such as CTX-M, with particular genotypes associated with different geographical regions. The spread of these enzymes has compromised the clinical utility of a number of beta-lactam classes and with the spread of genes such as bla(KPC), carbapenems may be increasingly compromised in the future. High-level fluoroquinolone resistance (mainly caused by gyrA mutations) has also been shown to be associated with CTX-M and CMY-type enzymes, commonly due to co-carriage on conjugative plasmids of the gene for the aminoglycoside-inactivating enzyme AAC-6(1)-Ib-cr and qnr genes (which confer low-level resistance), allowing the easy selection of gyrA mutants in the host strain. Resistance in Gram-positive bacteria is also widely distributed and increasing, with the emergence of community-associated methicillin-resistant Staphylococcus aureus (MRSA) blurring the distinction between hospital and community strains. Antibiotic use and environmental factors all have a role in the emergence and spread of resistance. This article reviews some of the new mechanisms and recent trends in the global spread of MDR bacteria.
Explaining Therapy Predictions with Layer-Wise Relevance Propagation in Neural Networks (2018)

In typical data analysis projects in biology and healthcare, simpler predictive models, such as regressions and decision trees, enjoy more popularity than more complex and expressive ones, such as neural networks. One reason for this is that the functioning of simpler models is easier to explain, which greatly increases user acceptance. A neural network, on the contrary, is often regarded as a black box model, because its very strength in modeling complex interactions also makes its operation almost impossible to explain. Still, neural networks remain very interesting tools, since they have demonstrated promising performance in a variety of predictive tasks, such as medical image classification and segmentation, as well as clinical event prediction, i.e., in the modeling of therapy decisions and survival time. In this work, we attempt to improve the explainability of neural networks applied in healthcare. We propose to apply the Layer-wise Relevance Propagation algorithm to explain clinical decisions proposed by deep modern neural networks. This algorithm is able to highlight the features that lead to the probabilistic prediction of therapy decisions for each individual patient. We evaluate the feature-oriented explanations generated by the algorithm with clinical experts. We show that the features, which are identified by the algorithm to be relevant, largely agree with clinical knowledge and guidelines. We believe that being able to explain machine learning based decisions greatly improves transparency and acceptance of neural network models applied in the clinical domain.
Impaired ATP Synthase Assembly Associated with a Mutation in the Human ATP Synthase Subunit 6 Gene (2001)

Mutations in human mitochondrial DNA are a well recognized cause of disease. A mutation at nucleotide position 8993 of human mitochondrial DNA, located within the gene for ATP synthase subunit 6, is associated with the neurological muscle weakness, ataxia, and retinitis pigmentosa (NARP) syndrome. To enable analysis of this mutation in control nuclear backgrounds, two different cell lines were transformed with mitochondria carrying NARP mutant mitochondrial DNA. Transformant cell lines had decreased ATP synthesis capacity, and many also had abnormally high levels of two ATP synthase sub-complexes, one of which was F(1)-ATPase. A combination of metabolic labeling and immunoblotting experiments indicated that assembly of ATP synthase was slowed and that the assembled holoenzyme was unstable in cells carrying NARP mutant mitochondrial DNA compared with control cells. These findings indicate that altered assembly and stability of ATP synthase are underlying molecular defects associated with the NARP mutation in subunit 6 of ATP synthase, yet intrinsic enzyme activity is also compromised.
A Simulation Study of the Combined Thermoelectric Extracellular Stimulation of the Sciatic Nerve of the Xenopus Laevis: The Localized Transient Heat Block (2012)

The electrical behavior of the Xenopus laevis nerve fibers was studied when combined electrical (cuff electrodes) and optical (infrared laser, low power sub-5 mW) stimulations are applied. Assuming that the main effect of the laser irradiation on the nerve tissue is the localized temperature increase, this paper analyzes and gives new insights into the function of the combined thermoelectric stimulation on both excitation and blocking of the nerve action potentials (AP). The calculations involve a finite-element model (COMSOL) to represent the electrical properties of the nerve and cuff. Electric-field distribution along the nerve was computed for the given stimulation current profile and imported into a NEURON model, which was built to simulate the electrical behavior of myelinated nerve fiber under extracellular stimulation. The main result of this study of combined thermoelectric stimulation showed that local temperature increase, for the given electric field, can create a transient block of both the generation and propagation of the APs. Some preliminary experimental data in support of this conclusion are also shown.
Magnetic printing technique for perpendicular thin-film media with coercivity of up to 10 000 Oe (2003)

A magnetic printing technique designed for perpendicular thin-film media using a lithographically formed master disk has been presented. It is shown that magnetic printing using perpendicular hard-disk media with high coercivities of up to 10 000 Oe is satisfactorily carried out and practical printing conditions for perpendicular recording media are discussed by analyzing differences in the magnetization processes during printing processes designed for perpendicular and longitudinal media.
Privacy-preserving targeted mobile advertising:A Blockchain-based framework for mobile ads (2020)

The targeted advertising is based on preference profiles inferred via relationships among individuals, their monitored responses to previous advertising and temporal activity over the Internet, which has raised critical privacy concerns. In this paper, we present a novel proposal for a Blockchain-based advertising platform that provides: a system for privacy preserving user profiling, privately requesting ads from the advertising system, the billing mechanisms for presented and clicked ads, the advertising system that uploads ads to the cloud according to profiling interests, various types of transactions to enable advertising operations in Blockchain-based network, and the method that allows a cloud system to privately compute the access policies for various resources (such as ads, mobile user profiles). Our main goal is to design a decentralized framework for targeted ads, which enables private delivery of ads to users whose behavioral profiles accurately match the presented ads, defined by the ad system. We implement a POC of our proposed framework i.e. a Bespoke Miner and experimentally evaluate various components of Blockchain-based in-app advertising system, implementing various critical components; such as, evaluating user profiles, implementing access policies, encryption and decryption of users' profiles. We observe that the processing delay for traversing policies of various tree sizes, the encryption/decryption time of user profiling with various key-sizes and user profiles of various interests evaluates to an acceptable amount of processing time as that of the currently implemented ad systems.<br><br>
CÉLÉBRATIONS NATIONALES 2004 (Book) (2004)

The involvement of hematological tumors such as lymphoma in the kidneys is a well-recognized phenomenon. Some of the distinct reported pathological processes resulting in kidney dysfunction include minimal change disease, lymphocytic invasion of the parenchyma, immune complex disposition, immunotactoid glomerulopathy, membranous glomerulopathy, and acute tubular injury. We report a rare case of CD20-negative intravascular lymphoma found on a kidney biopsy in a male with primary angiitis of the central nervous system (CNS) who presented with acute kidney injury and proteinuria. After the initiation of rituximab, cyclophosphamide, doxorubicin hydrochloride, vincristine sulfate, and prednisone (R-CHOP), kidney function improved and proteinuria resolved.
Frameshift Mutations in the Lactose Operon of E. coli (1966)

Polar mutants, first described in the lactose operon of Escherichia coli by Jacob and Monod (1961a, b) and Franklin and Luria (1961), have played an essential role in the development of the concept of the operon as a unit of coordinate genetic expression. This class of mutation, occurring within a structural gene (in contrast to mutations in operator or regulator genes) and having the unusual property of affecting the expression of genes other than the mutated gene, was a clear violation of the “one gene-one enzyme” postulate of Beadle and Tatum (1941). Polar mutations in the structural gene for β-galactosidase lead to a decreased synthesis of β-galactoside permease and thiogalactoside transacetylase.
Study Profile: The Japan “Society and New Tobacco” Internet Survey (JASTIS): A Longitudinal Internet Cohort Study of Heat-Not-Burn Tobacco Products, Electronic Cigarettes, and Conventional Tobacco Products in Japan (2018)

The JASTIS study provides the first estimates for heat-not-burn tobacco use in the world and e-cigarette use in Japan. For information on collaboration, please contact the corresponding author.