gebhart/patrepeval huggingface dataset:
The Behavior of Optic Axons on Substrate Gradients of Retinal Basal Lamina Proteins and Merosin (1996)

To study the behavior of optic axons to continuously changing concentrations of their substrate, explants from embryonic retina were placed across gradients of retinal basal lamina proteins and merosin. The following growth patterns of axons in response to the substrate gradients were found: (1) Axons that grew up gradients, i.e., from low to high substrate concentrations, became longer and less fasciculated with increasing concentration of the substrate. On shallow basal lamina gradients, the axons also showed a directional response that resulted in guidance to higher substrate concentrations. (2) Axons that grew down gradients, i.e., from high to low substrate concentrations, became shorter and more fasciculated with decreasing concentrations of the substrate. On gradients of merosin, a significant alteration in the axonal growth direction toward higher substrate concentrations was detected. Axons heading down gradients never U turned to higher substrate concentrations. (3) Axons confronted with discontinuous substrates were confined to the borders of the substrate exclusively, whereas axons confronted with substrate gradients were able to cross into the territory beyond the substrate. (4) The growth patterns of axons on substrate gradients of basal lamina proteins and merosin were similar but not identical, indicating that axons may respond to substrate gradients dependent on its chemical composition. The present results show that substrate gradients can regulate length and fasciculation of neurites and have a limited capability to direct axons to higher substrate concentrations.
UWB joint TOA and DOA estimation (2009)

This paper deals with the simultaneous estimation of Time of Arrival (TOA) and Direction of Arrival (DOA) in UWB systems considering multipath propagation. The estimation is performed in the frequency domain by computing a two dimensional power delay-angle spectrum based on the periodogram because of its low computational complexity. High accuracy is obtained in the estimation of both parameters, TOA and DOA, based on the wide bandwidth of the UWB signals.
Bile Salt-Stimulated Lipase of Human Milk (1987)

Human milk contains a lipase (bile salt-stimulated lipase) that is considered to have an important role in infant fat digestion. In this study we compared the characteristics of bile salt-stimulated lipase activity in milk samples from mothers delivering prematurely (26-30 and 31-37 weeks of gestation) and in milk from mothers delivering at term (38-42 weeks of gestation). Preterm milks were collected at day 1-5 and during week 6 of lactation. Term milks were collected during week 6 of lactation. The characteristics of the enzyme (kinetics, enzyme concentration, pH optimum, and pH stability, effects of bile salt structure and concentration, eserine inhibition) were identical regardless of length of pregnancy or duration of lactation. Bile salt-stimulated lipase had a neutral to alkaline pH optimum (pH 7.3-8.6), was stable for 1 h at a wide pH range (pH 3.1-8.6), was active only in the presence of primary bile salts, and was inhibited by eserine. The data indicate that, following parturition at as early as 26 weeks of gestation, the mammary glands synthesizes bile salt-stimulated lipase with identical characteristics as does the mammary gland after a full-term pregnancy.
Transient Expression of the Beta Interferon Promoter in Human Cells (1987)

A human transient expression assay was used to examine the inducible transcriptional activation of beta interferon (IFN-beta) and IFN-alpha 1 promoters in a homologous cellular environment. Use of 293 cells, an adenovirus DNA-transformed human embryonic kidney cell line, permitted Sendai virus-inducible expression of IFN-beta-CAT hybrid gene. Introduction of the simian virus 40 (SV40) enhancer 5' or 3' to the IFN-CAT gene increased basal (uninduced) levels of chloramphenicol acetyltransferase (CAT) activity; in one construct the SV40 enhancer--IFN-beta regulatory region combination increased the induced CAT activity 50- to 100-fold, suggesting that this may be a generally useful inducible enhancer-promoter combination. No expression from the IFN-alpha-CAT hybrid gene was detected in 293 cells, indicating that human epithelioid cells lack a factor required for expression of the IFN-alpha promoter. However, when the IFN-alpha regulatory region was combined with the SV40 enhancer, a low level of inducible CAT activity was detected in the human transient system.
Identification of a Novel Homozygous Multi-Exon Duplication in <i>RYR2</i> Among Children With Exertion-Related Unexplained Sudden Deaths in the Amish Community (2020)

In this analysis, we identified a novel, highly penetrant, homozygous multiexon duplication in RYR2 among Amish youths with exertion-related sudden death and sudden cardiac arrest but without an overt phenotype that is distinct from RYR2-mediated catecholaminergic polymorphic ventricular tachycardia. Considering that no cardiac tests reliably identify at-risk individuals and given the high rate of consanguinity in Amish families, identification of unaffected heterozygous carriers may provide potentially lifesaving premarital counseling and reproductive planning.
Mullitization from a Multicomponent Oxide System in the Temperature Range 1200°–1500°C (2000)

Mullitization from a multicomponent oxide system (alumina–kaolin–quartz–feldspar–talc) was analyzed as a function of firing temperature from 1200° to 1500°C based on quantitative XRD and SEM. In the present study, whisker forms of mullite grew in three characteristic stages. In the first stage (1255°–1295°C), mullitization (nucleation) took place from glass via alumina dissolution into glass under the condition of no apparent change in glass content. The reaction in this stage was rate‐limited by alumina dissolution into glass. Extensive mullitization occurred in the 1295°–1335°C range (second stage) directly from glass. Unlike in the sol–gel‐based binary system, alumina dissolution into glass was not shown to be the rate‐controlling mechanism during this extensive mullitization stage. Finally (>1335°C, third stage), the reaction was saturated, accompanied by an apparent decrease in glass consumption rate. The impingement of mullite whiskers by other whiskers and crystals was speculated to cause mullite to grow in the transverse direction, yielding a diminished reaction rate in the final stage. Mullitization stages in this work were compared with those of the alumina–silica binary system shown in the literature.
MEASUREMENT METHOD FOR SENSITIVITY ANALYSIS OF PROXIMITY SENSOR AND SENSOR ANTENNA INTEGRATION IN A HANDHELD DEVICE (2011)

A method for measuring the sensitivity of a capacitive proximity sensor and an application using the sensor as a proximity detector in mobile phone antennas is presented. 2D sensor data plots were physically more exact for tuning sensor placement. 3D sensor data plots were suitable for sensor intensity comparison, highlighting sensor difierences in multiple sensor applications and efiects in sensor's output due to shadowing mechanical objects. The antenna proximity sensor was measured and optimised with sensitivity measurements. In a PIFA application the antenna load could be detected from both sides and from above the antenna on a scale of 4:03 ¢ 10 i14 F to 4:33 ¢ 10 i14 F. The cases present all possible positions of holding a phone used in either the \calling or \browsing mode. The method and the application emphasise the physical sensitivity and electrical flelds of the sensor. The characteristics can be further improved by using other sensor types, sensor data fusion and advanced imitation of multisensory spatial interaction by humans and animals.
NEW CAPACATIVE STORAGE DEVICE (2009)

Ionic polymer metal composites (IPMCs) are one of the most new and promising materials being studied for their electromechanical properties. They are robust, light-weight, fabricated easily and into nearly any size or shape, and can produce large macro bending deformations while under low driving voltages or conversely produce measurable electric signals (sensing) when deformed (1). Our recent discovery of capacitive behaviour in dry prepared IPMCs has lead to development of high energy density devices. Here we report the use simple microfabrication of electrodes to produce ionomer composites sandwiched between two thin metal films. These composites exhibit capacitance per unit planar area of ~1.0 mF cm -2 (40 mF/g) for the ~127 µm-thick substrate. We further report on the scalability of these devices. These composites could open the door to high energy density flexible and scalable thin films needed to meet energy storage solutions of tomorrow.
A Study on Authentication Algorithm for NFC Security Channel (2012)

Recently, applications range of NFC is widening by popularization of smartphone. Expansion of NFC means generalization of electronic payments systems. So security of NFC is very important. AES-128 is safe cryptographic technique for NFC now in use. But, the more range of applications increases, the more safe cryptographic techniques are necessary. In this paper, we propose the safe method is unaffected by the development of NFC. Proposed A-NFC scheme, adding the authentication of asymmetric cryptographic, is easy to apply for NFC and NFC-USIM chipsets, and it can adapt to the general NFC environment.
Vigorous Response of Human Innate Functioning IgM Memory B Cells upon Infection by <i>Neisseria gonorrhoeae</i> (2012)

Neisseria gonorrhoeae, the cause of the sexually transmitted infection gonorrhea, elicits low levels of specific Ig that decline rapidly after the bacteria are cleared. Reinfection with the same serovar can occur, and prior gonococcal infection does not alter the Ig response upon subsequent exposure, suggesting that protective immunity is not induced. The mucosal Ig response apparent during gonorrhea does not correlate with that observed systemically, leading to a suggestion that it is locally generated. In considering whether N. gonorrhoeae directly influences B cells, we observed that gonococcal infection prolonged viability of primary human B cells in vitro and elicited robust activation and vigorous proliferative responses in the absence of T cells. Furthermore, we observed the specific expansion of IgD(+)CD27(+) B cells in response to gonococcal infection. These cells are innate in function, conferring protection against diverse microbes by producing low-affinity, broadly reactive IgM without inducing classical immunologic memory. Although gonococcal infection of B cells produced small amounts of gonococcal-specific IgM, IgM specific for irrelevant Ags were also produced, suggesting a broad, polyspecific Ig response. The gonococci were effectively bound and engulfed by B cells. TLR9-inhibitory CpGs blocked B cell responses, indicating that intracellular bacterial degradation allows for innate immune detection within the phagolysosome. To our knowledge, this is the first report of a bacterial pathogen having specific affinity for the human IgM memory B cells, driving their potent activation and polyclonal Ig response. This unfocused T-independent response explains the localized Ig response that occurs, despite an absence of immunologic memory elicited during gonorrhea.
Design, testing and analysis of journal bearings for construction equipment (2005)

Grease-lubricated journal bearings present a common challenge for construction equipment manufacturers in the world. The common design methodology is based on empirical data and has worked very well historically because the market and governments have accepted that bearings in construction equipment need frequent lubrication and exchange of worn parts. Legal and market requirements will soon demand lower environmental impact and increased machine efficiency. These requirements call for better methods of designing grease lubricated journal bearings. The goal of the outlined work was to develop better design methods for grease lubricated journal-bearing design used in heavy-duty construction equipment machines, in order to prolong life and lubrication intervals. The research approach of the project can roughly be divided into three phases: 1. Development of test apparatus and test methods for journal bearing studies. 2. Bench tests of grease lubricated journal bearing design. 3. Verification between bench tests and computer simulations. In the thesis the current state of the art in bearing design for construction equipment is discussed and summarized in the form of design guidelines. The suggested design steps are just a mean to get to the starting point of design. The simple guidelines do however serve a purpose when collected since most published bearing design guidelines are aimed at the bushing material or at continuously rotating bearings. The influence of housing, environment and load cases can not be ignored when designing a bearing. Long term field-testing and experience can not be replaced until better design criteria have been established. Paper A deals with the design of the bearing test apparatus that was built and evaluated. Comparisons between theoretical contact and contact elements in Finite Element program have been made and discussed in paper B. In paper C a replica technique for measuring wear of large field specimens was evaluated. A case study of bearing housing design was performed in paper D utilizing Finite Element program and then validated in paper E in the bearing test apparatus. The influence of grease groove design on bushing life was tested and evaluated in paper F. Wear simulation of a plain bushing has been performed with a Finite Element program and presented in paper G.
Particle tracking by deformable templates (2002)

Describes an approach to particle tracking based on deformable templates. Hough transforms are used to give initial conditions for the templates which then converge using a deterministic annealing algorithm. This template approach is closely related to the elastic net algorithm. The authors demonstrate successful results on both simulated and real data. The authors also show how the Hough transform can be derived from the deformable template algorithm as a special case in the low temperature (or gradient descent limit).< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Complement Activation by C-Reactive Protein on the HEp-2 Cell Substrate (1996)

The complement (C) activation by C-reactive protein (CRP) in acute-phase sera is routinely tested in our laboratory by means of an indirect immunofluorescence method (C3-IFT) on rat kidney sections. This C3-IFT assay is based on the binding of CRP to the renal tissue followed by the fixation of C4 and C3 components to distinct vessel-associated medullary structures as a result of CRP-mediated C activation in vitro. While the activation cascade leading to the deposition of C4 and C3 could previously be deduced experimentally, we were unable as yet to visualize CRP and the components of the C1 complex on kidney sections when testing patients' sera by indirect immunofluorescence. In an attempt to analyze the mechanisms of unexpectedly negative C3-IFT results (e.g. bacterial endocarditis) we employed monolayers of fixed HEp-2 cells which have previously been shown to be a suitable substrate for CRP binding. By incubating purified native CRP supplemented with normal human serum as a source of C we detected the C components Clq, Clr, Cls, C4 and C3 in the same speckled immunofluorescent pattern on HEp-2 cell nuclei as described characteristically for CRP binding. The serial activation steps from CRP up to C3 could also be followed on HEp-2 cells using C3-IFT-positive acute-phase sera. However, certain C3-IFT-negative acute-phase sera showed an arrest between Cls and C4 of the CRP-mediated C activation cascade. HEp-2 cells can thus be used to monitor the process of autologous C activation initiated by endogeneous CRP in patients' sera. In contrast to native CRP, urea-modified CRP (mCRP) did not bind to HEp-2 cell nuclei, but was detected in association with distinct filamentous cytoplasmic structures. Unlike its native counterpart, binding of mCRP was not followed by a deposition of C components.
Monitoring pH-Triggered Drug Release from Radioluminescent Nanocapsules with X-ray Excited Optical Luminescence (2013)

One of the greatest challenges in cancer therapy is to develop methods to deliver chemotherapy agents to tumor cells while reducing systemic toxicity to noncancerous cells. A promising approach to localizing drug release is to employ drug-loaded nanoparticles with coatings that release the drugs only in the presence of specific triggers found in the target cells such as pH, enzymes, or light. However, many parameters affect the nanoparticle distribution and drug release rate, and it is difficult to quantify drug release in situ. In this work, we show proof-of-principle for a "smart" radioluminescent nanocapsule with an X-ray excited optical luminescence (XEOL) spectrum that changes during release of the optically absorbing chemotherapy drug, doxorubicin. XEOL provides an almost background-free luminescent signal for measuring drug release from particles irradiated by a narrow X-ray beam. We study in vitro pH-triggered release rates of doxorubicin from nanocapsules coated with a pH-responsive polyelectrolyte multilayer using HPLC and XEOL spectroscopy. The doxorubicin was loaded to over 5% by weight and released from the capsule with a time constant in vitro of ∼36 days at pH 7.4 and 21 h at pH 5.0, respectively. The Gd₂O₂S:Eu nanocapsules are also paramagnetic at room temperature with similar magnetic susceptibility and similarly good MRI T₂ relaxivities to Gd₂O₃, but the sulfur increases the radioluminescence intensity and shifts the spectrum. Empty nanocapsules did not affect cell viability up to concentrations of at least 250 μg/mL. These empty nanocapsules accumulated in a mouse liver and spleen following tail vein injection and could be observed in vivo using XEOL. The particles are synthesized with a versatile template synthesis technique which allows for control of particle size and shape. The XEOL analysis technique opens the door to noninvasive quantification of drug release as a function of nanoparticle size, shape, surface chemistry, and tissue type.
Antisense Mapping of the c- <i>fos</i> Promoter: Role of the Serum Response Element (1991)

Using an antisense RNA approach to eliminate endogenous expression of the c-fos protein, we have verified by nuclear run-on and transient expression assays that the Fos protein is a negative regulator of its own transcription in vivo. The negative autoregulation of the c-fos promoter by Fos was further confirmed by overexpression of an antisense-resistant c-fos expressing vector. Antisense mapping of the c-fos promoter demonstrated that the serum responsive element (SRE) represents the major site for c-fos suppression only during the first hour, but that additional adjacent DNA sequences are required for suppression at later times. We propose that antisense inhibition of transcriptional repressors provides a useful method for analyzing the significance and mechanism of transcriptional repression in vivo.
Legionella spp.: community acquired and nosocomial infections (2003)

If domestic aquatic reservoirs were eventually confirmed as significant agents of transmission of legionellosis, the adoption of preventive measures would then be crucial. The progressive identification of other species, different from L. pneumophila, as causative agents of pneumonia should both encourage microbiologists and clinicians to improve their diagnostic methodology and increase the awareness of these infections. Finally, the awareness of mixed infections, probably far more severe and perhaps not so uncommon as previously thought, has important clinical connotations for both the diagnostic and the therapeutic approach to legionellosis in the immunosuppressed host, particularly in those cases of delayed clinical resolution.
Human myeloma cells acquire resistance to difluoromethylornithine by amplification of ornithine decarboxylase gene (1987)

Stepwise increments of the concentration of 2-difluoromethylornithine (DFMO), a mechanism-based irreversible inhibitor of mammalian ornithine decarboxylase (ODC), resulted in a selection of cultured human IgG-myeloma cells (Sultan cell line) capable of growing in the presence of up to 3 mM-DFMO. This capacity was associated with 10-fold increase in ODC activity in the dialysed extracts of drug-resistant myeloma cells, markedly enhanced synthesis rate for ODC enzyme molecules, as revealed by a 20 min [35S]methionine labelling of cellular proteins, followed by specific immunoprecipitation and SDS/polyacrylamide-gel electrophoresis, dose-dependently increased expression of ODC mRNA in resistant cells (effective dose causing 50% inhibition), dose-dependent amplification of ODC gene sequences in a 9-kilobase-pairs EcoRI genomic DNA fragment, and (v) a 10-fold increase in the ED50 (effective dose causing 50% inhibition) for the anti-proliferative action of DFMO in these myeloma cells. These results represent one of the few gene amplifications described in cultured human cells.
Extending the Lean Limit of Combustion of Spark Ignition Engines Operating on CNG (1992)

The effect of charge stratification on lean mixture combustion in a Ricardo research engine has been investigated. To do this, a commercially available spark plug was modified so that small amounts of CNG or methane gas could injected, via the spark electrode, into a lean mixture within the combustion chamber prior to ignition. The effect on engine performance of variations the methane injection process were analysed. To date the research has led the following conclusions: The lean limit of a homogeneous air-fuel mixture is extended by this relatively simple charge stratification process. The effectiveness of charge stratification is most noticeable at lean air-fuel ratios in terms of improved brake specific fuel consumption. Unburnt hydrocarbon emission levels are higher with the stratified charge process when compared to normal homogeneous mixture operation. Carbon monoxide levels with stratified charge combustion are almost same or a little lower than normal operation at the leanest air-fuel ratios. Work is continuing to determine the effect of the stratified charge Ignition process on the lower limit of flammability of methane.
Agriculture: A Key to Adequate Food and Nutrition the People of the World (1981)

Congressman Brown, a native of the southern California desert town of Holtville in the Imperial Valley, represents the 36th District in the Riverside-San Bernardino area of California, in the House of Representatives. He serves on the House Agriculture Committee, and is Chairman of its Subcommittee on Department Operations, Research, and Foreign Agriculture. Congressman Brown is well known for his knowledge in agriculture and Us relation to the world's nutrition needs
Induction de la fermentation malolactique des vins par inoculation d'une biomasse industrielle congelée de <em>L. oenos</em> après réactivation (1983)

<p style="text-align: justify;">Stimulation of malolactic fermentation can be obtained by adding to wine an industrial biomass of <em>L. oenos</em>. Before use, deepfreeze bacteria need to be reactivated according to some special conditions which have been studied. Yeasts concerned in alcoholic fermentation have an influence on malolactic fermentation capacity of wines ; this fact is in connection with some differences observed in wine technology. Among the strains of commercial dry yeasts, the influence of <em>S. cerevisiae</em> is lower than that of <em>S. bayanus</em>.</p>