gebhart/patrepeval huggingface dataset:
Mutator strains of Escherichia coli, mutD and dnaQ, with defective exonucleolytic editing by DNA polymerase III holoenzyme. (1983)

The closely linked mutD and dnaQ mutations confer a vastly increased mutation rate on Escherichia coli and thus might define a gene with a central role in the fidelity of DNA replication. To look for the biochemical function of the mutD gene product, we have measured the 3' leads to 5' exonucleolytic editing activity of polymerase III holoenzyme from mutD5 and dnaQ49 mutants. The editing activities of the mutant enzymes are defective compared to wild type, as judged by two assays: (i) decreased excision of a terminal mispaired base from a copolymer substrate and (ii) turnover of dTTP to dTMP during replication with a phage G4 DNA template. Thus, the mutD (dnaQ) gene product is likely to control the editing (proofreading) capacity of polymerase III holoenzyme.
Virtual Environments Research at the Georgia Tech GVU Center (1993)

August 01 1993 Virtual Environments Research at the Georgia Tech GVU Center Larry F. Hodges, Larry F. Hodges Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Search for other works by this author on: This Site Google Scholar Jay Bolter, Jay Bolter Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Search for other works by this author on: This Site Google Scholar Elizabeth Mynatt, Elizabeth Mynatt Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Search for other works by this author on: This Site Google Scholar William Ribarsky, William Ribarsky Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Search for other works by this author on: This Site Google Scholar Ron van Teylingen Ron van Teylingen Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Search for other works by this author on: This Site Google Scholar Author and Article Information Larry F. Hodges Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Jay Bolter Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Elizabeth Mynatt Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. William Ribarsky Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. Ron van Teylingen Graphics, Visualization & Usability Center, Georgia Institute of Technology, Atlanta, GA 30332. © 1994 The Massachusetts Institute of Technology1994 Presence: Teleoperators and Virtual Environments (1993) 2 (3): 234–243. https://doi.org/10.1162/pres.1993.2.3.234 Cite Icon Cite Permissions Share Icon Share Twitter LinkedIn Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Search Site Citation Larry F. Hodges, Jay Bolter, Elizabeth Mynatt, William Ribarsky, Ron van Teylingen; Virtual Environments Research at the Georgia Tech GVU Center. Presence: Teleoperators and Virtual Environments 1993; 2 (3): 234–243. doi: https://doi.org/10.1162/pres.1993.2.3.234 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsPRESENCE: Virtual and Augmented Reality Search Advanced Search This content is only available as a PDF. © 1994 The Massachusetts Institute of Technology1994 Article PDF first page preview Close Modal You do not currently have access to this content.
Analysis of effect of congenic mice on the gene expression under genomic background of BALB/c mice (2014)

Background Interleukin 1 (IL-1) is a major contributor to the development of immune-mediated arthritis. BALB/c mice that are homozygous for the deficiency (BALB/c) spontaneously develop joint-specific inflammation that resembles human rheumatoid arthritis [1]. To understand the role of genetic factors involved in the development of spontaneous arthritis (SAD) in mice deficient in IL-1 receptor antagonist protein (IL_1RA), we have identified a genomic region containing a major quantitative trait locus (QTL) for this disease. The QTL is on chromosome 1 and appears to be the strongest genetic region regulating arthritis [2]. To confirm the importance of this QTL in SAD, we next developed congenic mouse strains that contain the strong QTL genomic fragments from BALB/c on a DBA/1 genetic background [3] and conversely a similar genomic fragment from DBA/1 on a BALB/c background. When the DBA/1 fragment was placed on a BALB/c background, arthritis was delayed and less severe. When the BALB/c fragment was placed on a DBA/1 background, arthritis occurred in varying degrees.
cAMP signal transduction induces eNOS activation by promoting PKB phosphorylation (2006)

The objective of this study was to determine whether activation of protein kinase B (PKB) is involved in the production of nitric oxide (NO) induced by cAMP signal transduction. Mongrel dogs were used for this study. Coronary microvessels were isolated from the left ventricular free wall of these dog hearts. Forskolin (an activator of adenylyl cyclase that increases intracellular cAMP level) and 8-bromo-cAMP (a membrane-permeable cAMP analog) were used to stimulate NO release and activation of PKB and endothelial NO synthase (eNOS) in these blood vessels. We found that forskolin and 8-bromo-cAMP increased NO release (quantified by using the Griess reaction) from coronary microvessels by 80 +/- 6 and 78 +/- 11 pmol/mg (mean +/- SE), respectively (P < 0.05 vs. control). Western blot analysis showed that forskolin elicited a significant increase in eNOS phosphorylation (59 +/- 11%) at serine-1177 (a positively regulatory phosphorylation site for eNOS) and a significant increase in dephosphorylation (28 +/- 6%) at threonine-495 (a negatively regulatory phosphorylation site of eNOS) (P < 0.05 vs. control). Interestingly, forskolin also increased the phosphorylation of PKB at serine-473 (by 49 +/- 17%) and threonine-308 (by 53 +/- 17%), respectively (P < 0.05 vs. control; phosphorylation of both sites is required for a full activation of PKB). N(omega)-nitro-l-arginine methyl ester (an NOS inhibitor) blocked NO formation, Rp diastereomer of cAMP (a PKA inhibitor), and LY-294002 [a PI3-kinase (an activator of PKB) inhibitor] prevented the production of NO, phosphorylation of PKB, and eNOS induced by forskolin. Our data clearly show an involvement of PKB activation in cAMP signal-induced NO production. We are reporting for the first time that cAMP signal transduction stimulates eNOS activation through a PKB-mediated mechanism.
Effect of V-defects on the performance deterioration of InGaN/GaN multiple-quantum-well light-emitting diodes with varying barrier layer thickness (2013)

The effect of quantum barrier (QB) thickness on performances of InGaN/GaN multiple-quantum-well light-emitting diodes (MQW LEDs) with relatively large barrier layer thicknesses has been investigated. It is found that the density and averaged size of V-defects increases with QB thickness, resulting in larger reverse- and forward-bias current in LEDs. Electroluminescence measurement shows that LED with thinner QB has higher internal quantum efficiency but lower efficiency droop-onset current density, which should be ascribed to the faster saturation of carrier leakage into V-defects. Correspondingly, above the droop-onset current density, severer Auger recombination and carrier overflow are induced by higher carrier density due to the less V-defect related carrier leakage, leading to the more serious droop phenomenon in LEDs with thinner QB.
Differences in teratogenic and toxic properties of alcohol dehydrogenase inhibitors pyrazole and 4‐methylpyrazole in <i>Drosophila melanogaster</i>. II. ADH allozymes in an isogenic background (1994)

Pyrazole and 4-methylpyrazole (4-MP) are in vivo and in vitro inhibitors of alcohol dehydrogenase activity in mammals. The fruitfly Drosophila melanogaster has been used to demonstrate the influence of genetic variation in alcohol dehydrogenase alleles on the results of larval treatment with pyrazole and 4-MP. Genetic polymorphism of organisms involved in experiments with teratogenic and toxic agents is not often considered. Administration of pyrazole to larvae of isogenic D. melanogaster strains, differing mainly in their Adh alleles, caused large Notch-like teratogenic aberrations, macrochaetae multiplication, and pupal mortality. The level of teratogenicity and developmental-toxicity of pyrazole was both concentration and Adh-genotype-dependent. The strain with the highest ADH activity showed smaller effects after the treatments with the two concentrations used. 4-MP does not cause morphological aberrations, although treatment of larvae with an isogenic background caused a high pupal mortality due to non-differentiated material in the pupal case.
Targeting thrombomodulin to circulating red blood cells augments its protective effects in models of endotoxemia and ischemia‐reperfusion injury (2016)

ABSTRACT Endothelial thrombomodulin (TM) regulates coagulation and inflammation via several mechanisms, including production of activated protein C (APC). Recombinant APC and soluble fragments of TM (sTM) have been tested in settings associated with insufficiency of the endogenous TM/APC pathway, such as sepsis. We previously designed a fusion protein of TM [single‐chain variable fragment antibody (scFv)/TM] targeted to red blood cells (RBCs) to improve pharmacokinetics and antithrombotic effects without increasing bleeding. Here, scFv/ TM was studied in mouse models of systemic inflammation and ischemia‐reperfusion injury. Injected concomitantly with or before endotoxin, scFv/TM provided more potent protection against liver injury and release of pathological mediators than sTM, showing similar efficacy at up to 50‐fold lower doses. scFv/TM provided protection when injected after endotoxin, whereas sTM did not, and augmented APC production by thrombin ~50‐fold more than sTM. However, scFv/TM injected after endotoxin did not reduce thrombin/antithrombin complexes; nor did antibodies that block APC anticoagulant activity suppress the prophylactic anti‐inflammatory effect of scFv/TM. Therefore, similar to endogenous TM, RBC‐anchored scFv/TM activates several protective pathways. Finally, scFv/TM was more effective at reducing cerebral infarct volume and alleviated neurological deficits than sTM after cerebral ischemia/reperfusion injury. These results indicate that RBC‐targeted scFv/TM exerts multifaceted cytoprotective effects and may find utility in systemic and focal inflammatory and ischemic disorders.—Carnemolla, R., Villa, C.H., Greineder, C. F., Zaitseva, S., Patel, K.R., Kowalska, M.A., Atochin, D. N., Cines, D.B., Siegel, D.L., Esmon, C. T., Muzykantov, V. R. Targetingthrombomodulin to circulatingred blood cells augments its protective effects in models of endotoxemia and ischemia‐reperfusion injury FASEB J. 31, 761–770 (2017). http://www.fasebj.org
Contact resistance response surface of sintered Al films on (100) silicon (1979)

Al–Si contact resistance for (100) p-type Si was studied as a function of sintering gas ambient, temperature, and time. Variables were N2 vs forming gas (10%H2, 90%N2); 540°C, 500°C, 450°C; 10, 20, 30, and 40 minutes. Results showed that forming gas yields higher contact resistance values than does N2. Away from the Al–Si eutectic point, time and temperature did not appear to be significant variables. The quantum mechanical electron tunneling model for metal–semiconductor contact resistance developed by Yu in 1970 is briefly described. This model predicts very well the range of contact resistance values measured in this experiment. The effects of forming gas can be understood by the addition of a space charge due to H2 diffusion into the Al film.
Improved high volume air sampler (1974)

Sampler permits size separations of particles by directing sampled air through cross-sectional area sufficiently large that air velocity is reduced to point where particles or larger size will settle out. Sampler conducts air downward and through slots around periphery of unit into relatively open interior of house.
Active Trifluoromethylating Agents from Well-Defined Copper(I)−CF<sub>3</sub> Complexes (2008)

The first examples of isolable and structurally characterized Cu(I)-CF3 complexes are reported. N-Heterocyclic carbene (NHC)-supported copper tert-butoxide complexes reacted with Me3Si-CF3 to afford new (NHC)Cu-CF3 complexes whose structures were dependent on whether or not the C4-C5 positions of the five-membered NHC rings were saturated. In situ generated (SIiPr)Cu-CF3 cleanly transferred its trifluoromethyl group to a number of organic halides under mild conditions.
<sup>89</sup>Zr-Oxine Complex PET Cell Imaging in Monitoring Cell-based Therapies (2015)

We have developed a (89)Zr-oxine complex cell tracking technique for use with PET that is applicable to a broad range of cell types and could be a valuable tool with which to evaluate various cell-based therapies.
Microbiological, Sensory and Chemical Characteristics of Vacuum‐Packaged Ground Beef Patties Treated with Salts of Organic Acids (1997)

ABSTRACT Vacuum‐packaged ground beef patties containing sodium lactate (NaL), sodium propionate (NaP), sodium acetate (NaA) and sodium citrate (NaC) at various levels and combinations were stored up to 28 days at 4°C. Addition of sodium lactate alone or in combination with sodium propionate increased shelf life of the patties by decreasing microbiological growth and decreasing negative flavor notes associated with lipid oxidation. Lean color also was improved by addition of sodium lactate alone or in combination with sodium propionate. Lipid oxidation by TBA was only slightly affected by treatment addition or storage.
Investigation of photoacoustic guidance of diffusive optical tomography (2008)

We report the first experimental investigations of photoacoustic guidance of diffusive optical tomography for detection and characterization of optical contrast targets. The hybrid system combined an 8-source, 10-detector reflection mode frequency domain DOT imager with either orthogonal and reflection-geometry photoacoustic systems. The PAT subsystems imaged two-dimensional cross-sections to define centers and radii of regions of interest for a dual-zone mesh DOT imaging algorithm. Phantom absorbers, 1 cm in diameter, of high and low contrast, were spaced 1.5 to 2.5 cm apart at depths ranging from 1 to 2 cm in a turbid medium. Without PAT guidance, the absorber DOT images in many cases were merged and indistinguishable. With PAT guidance, the two targets were well resolved and the reconstructed absorption coefficients improved to 86-130% of the true values. In addition, using both pulse-echo and photoacoustic image detection, the photoacoustic guidance correctly distinguished mechanical from optical contrast providing more specific target information and reconstruction accuracy.
Purine nucleoside phosphorylase from human erythrocytes: a kinetic study of the fully separated isoenzymes. (1991)

Human red cell lysates contain at least seven electrophoretically distinct isoenzymes of purine-nucleoside phosphorylase (PNPase); the proportion of more anodal bands increases as the erythrocyte ages, suggesting that the native enzyme is subjected to progressive post-translational modifications. The age dependent electrophoretic changes observed in the hemolysate are associated with the downward curvature of the Lineweaver-Burk double reciprocal plot at high inosine-substrate concentrations unlike the single-banded PNPase from tissue cultures of rapidly dividing cells. Thanks to the high resolution power of the ion-exchange HPLC technique utilized we have been able to fully separate all the seven isoenzymes and correlate structural to functional modifications in PNPase from human erythrocytes. Our results indicate that the downward curvature of Lineweaver-Burk plot is not due to a mixture of isoforms with low and high Km for inosine but that the allosteric activation by the inosine substrate is the direct consequence of structural modification(s) on the "primary" form of the enzyme.
On-line international cash management (1983)

This paper describes a state-of-the-art cash management system recently installed for the Treasury Section of the United Nations Development Programme (UNDP). This system was a multi-year development effort of the UNDP supported by Chemical Bank's Cash Management Consulting Services (CMCS). It is resident on an IBM 4331 running under CICS with a DOS/VSE operating system. The data base management system is DL/I and it utilizes IBM 3270 terminals locally.After a brief explanation on the basic functions of cash management, the general objectives of treasury systems are given to provide a background for the description of the system. The nature and scope of the operations of the Treasury Section are stated. In summary, the UNDP manages a cash flow of approximately d1 billion in over 150 currencies. Daily payments are made through 35 agencies with offices in 115 countries. Receivables (contributions) are denominated in 150 currencies. Cash in over 400 bank accounts is monitored daily and a d500,000,000 short-term portfolio, denominated in almost every major currency is maintained.
Acoustical pre-processing for robust speech recognition (1989)

In this paper we describe our initial efforts to make SPHINX, the CMU continuous speech recognition system, environmentally robust. Our work has two major goals: to enable SPHINX to adapt to changes in microphone and acoustical environment, and to improve the performance of SPHINX when it is trained and tested using a desk-top microphone. This talk will describe some of our work in acoustical pre-processing techniques, specifically spectral normalization and spectral subtraction performed using an efficient pair of algorithms that operate primarily in the cepstral domain. The effects of these signal processing algorithms on the recognition accuracy of the Sphinx speech recognition system was compared using speech simultaneously recorded from two types of microphones: the standard close-talking Sennheiser HMD224 microphone and the desk-top Crown PZM6fs microphone. A naturally-elicited alphanumeric speech database was used. In initial results using the stereo alphanumeric database, we found that both the spectral subtraction and spectral normalization algorithms were able to provide very substantial improvements in recognition accuracy when the system was trained on the close-talking microphone and tested on the desk-top microphone, or vice versa. Improving the recognition accuracy of the system when trained and tested on the desk-top microphone remains a difficult problem requiring more sophisticated noise suppression techniques.
Improved radioxenon detection techniques for use with fluid-based concentration (2000)

A portable system that monitors the quantity of radioxenon (/sup 131m/Xe, /sup 133/Xe, /sup 133m/Xe, and /sup 135/Xe) in the atmosphere is being developed. The integrated monitoring system consists of a fluid-based concentration sub-system and a detection sub-system. This study evaluates the performance of different high-efficiency geometries that use NaI(Tl) photon detectors along with with either gas proportional, plastic scintillator, or PIPS detectors to distinguish radioxenon signature emissions and discriminate against radon background. In addition, a NaI(Tl)/CsI(Tl) phoswich detector was investigated as an alternative photon detector. Finally, a dual parameter data acquisition system was demonstrated. Minimum detectable activity (MDA) for a six hour count time of /sup 133/Xe was used as the primary measure of performance. The lowest MDAs were 52/spl plusmn/7 mBq for the gas proportional/NaI(Tl) configuration, 48/spl plusmn/7 mBq for the plastic scintillator/NaI(Tl) configuration and 73/spl plusmn/8 mBq for the PIPS/NaI(Tl) configuration. Based on these results, the performance of a proposed final detection system is predicted to result in a MDA of 12.2 mBq without shielding. This detection system performance compares well to existing radioxenon detection systems and indicates the requirements of the concentration system.
Cloning and characterization of the P subunit of glycine decarboxylase from pea (Pisum sativum). (1992)

A pea leaf cDNA library constructed in lambda gt11 was screened with an antibody raised to the P subunit of glycine decarboxylase. One of the positive clones isolated was sequenced and shown to contain an open reading frame, which encoded the entire P subunit polypeptide. Aligning the deduced amino acid sequence with the amino acid sequence determined directly from the NH2 terminus of the mature P subunit shows the presence of a putative 86 amino acid leader sequence, presumably required for import into the mitochondria, and gives a Mr of the mature protein of 105,000. Comparison of this deduced amino acid sequence with the sequence of a pyridoxal phosphate-containing peptide isolated from the P subunit of chicken liver glycine decarboxylase shows remarkable conservation. The P subunit, however, shows little sequence homology with other published amino acid decarboxylases. Expression of the P subunit mRNA shows a pattern very similar to that of the corresponding polypeptide: it is strongly light induced and is expressed at a much higher level in leaves than in other tissues. Southern blot analysis suggests that the P subunit is encoded by a small multigene family.
Release of merozoite dense granules during erythrocyte invasion by Plasmodium knowlesi (1989)

We used immunoelectron microscopy to study the fate of dense granules during the invasion of erythrocytes by Plasmodium knowlesi merozoites. When merozoites entered host cells, dense granules moved to the pellicle, released their contents into the parasitophorous vacuole space, and then moved into fingerlike channels of the vacuole membrane. This is the first report showing that the content of dense granules of P. knowlesi is different from the contents of rhoptries and micronemes and is associated with the formation of channels from the parasitophorous vacuole.
B lineage‐restricted rearrangement of a human Ig<i>x</i> transgene (1997)

Recent drug-membrane interaction and quantitative structure-activity relationship studies of thioxanthenes and related compounds acting as multidrug resistance (MDR) modifiers pointed to the importance of the stereoisomery for their MDR reversing activity. Therefore a molecular modeling study of trans-(T) and cis-flupentixol (C) was performed in order to elucidate the observed discrepancy between equal binding potency to P-glycoprotein and different MDR reversing activity of the two stereoisomers. The results show that the 2 to 3-fold difference in MDR reversing activity of T compared to C might be related to a different orientation of the molecules in the membrane lipid environment. From the conformations generated by the SYBYL systematic search procedure those comprising local energy minima were selected and further optimized with semiempirical quantum chemistry methods. From the optimized conformations those that corresponded to 1H NMR results on drug conformations in lipid environment were selected for further molecular modeling studies. The electrostatic and lipophilic fields of T and C were compared in order to identify molecular properties related to the activity difference. The results show that the electrostatic fields of the drugs when similar in shape are dissimilar and that the lipophilic and hydrophilic regions are clearer separated in T in comparison with C. This imposes a better fitting of T compared to C to membrane lipid environment in accordance with the observed higher interaction strength of T with phospholipids.