gebhart/patrepeval huggingface dataset:
Stabilization of clathrin coated vesicles by amantadine, tromantadine and other hydrophobic amines (1991)

Amantadine and related compounds stabilized the structure of purified pig brain clathrin coated vesicles (CCV) at biologically relevant concentrations. Incubation of purified CCV for 30 min at 25 degrees C or 37 degrees C caused the release of clathrin, as determined by a centrifugation assay, and a reduction in the number of coated vesicles, by electron microscopy. Amantadine (10 mM), tromantadine (1 mM), amidine D295 (cyclohexylcarboximidamide-(N-benzyl)hydrochloride (10 mM), chloroquine (0.1 mM) and monodansylcadaverine (10 mM) significantly reduced the extent of dissociation.
Catalyst Performance in “Click” Coupling Reactions of Polymers Prepared by ATRP:  Ligand and Metal Effects (2006)

CuI-catalyzed azide−alkyne cycloadditions were conducted in organic media under various conditions. The effects of several parameters (ligand, solvent, reducing agent, metal) on these reactions were studied using the step-growth click coupling of low-molecular-weight α,ω-diazido-terminated polystyrene prepared by atom transfer radical polymerization (ATRP). These reactions were typically conducted in DMF, monitored by size exclusion chromatography (SEC), and semiquantitatively analyzed by Gaussian multipeak fitting and subsequent peak integration. Both the electronic properties of the ligand and the number of coordinating atoms had significant influence on the rates of the click coupling reactions. Aliphatic amine ligands led to significantly faster rates as compared to pyridine-based ligands. Faster rates were also observed with tridentate vs tetradentate ligands. A further rate enhancement was observed when the reactions were conducted in a noncoordinating solvent (toluene) vs a coordinating solvent (DMF). Despite the typical susceptibility of CuI complexes to oxidation, the addition of excess hydrazine as a reducing agent allowed click reactions to be conducted under limited amounts of air with decreased catalyst concentrations. A pronounced rate enhancement was observed during reactions conducted in the presence of hydrazine, which could be due to the basicity of hydrazine. Finally, azide−alkyne cycloadditions were successfully catalyzed by oxidatively stable metal complexes, including those of NiII, PdII, and PtII. The PtII catalyst demonstrated the highest catalytic activity relative to those of the other metals.
CDE Plug-and-Play (1998)

The programming infrastructure, such as ToolTalk, is a major strength of the Common Desktop Environment. This article illustrates client and server plug-and-play through the use of the Desktop's Application Programming Interfaces (APIs)</
A single-base change within the DNA polymerase locus of herpes simplex virus type 2 can confer resistance to aphidicolin (1987)

An aphidicolin-resistant (Aphr) mutant of herpes simplex virus (HSV) type 2 strain 186 previously has been shown to induce an altered viral DNA polymerase that is more resistant to aphidicolin and more sensitive to phosphonoacetic acid (PAA) than is wild-type DNA polymerase. In this study the mutation responsible for the aphidicolin-resistant phenotype was physically mapped by marker transfer experiments. The physical map limits for the Aphr mutation were contained in a 1.1-kilobase pair region within the HSV DNA polymerase locus. The 1.1-kilobase-pair fragment of the Aphr mutant also conferred hypersensitivity to PAA, and DNA sequence analysis revealed an AT to GC transition within this fragment of the Aphr mutant. Analysis of the three potential open reading frames within the 1,147-base-pair fragment and comparison with the amino acid sequence of DNA polymerase of HSV type 1 indicated that the Aphr mutant polymerase had an amino acid substitution from a tyrosine to a histidine in the well-conserved region of the DNA polymerase. These results indicate that this single amino acid change can confer altered sensitivity to aphidicolin and PAA and suggest that this region may form a domain that contains the binding sites for substrates, PPi, and aphidicolin.
ChemInform Abstract: Telluroaldehydes and Telluroketones. (1990)

Abstract Bis(dimethylaluminum) telluride (III), prepared by transmetalation, can serve as an efficient tellurating reagent for the direct conversion of aldehydes (IV) and ketones (VII) or (X) into the title compounds, trapped by dienes as the cycloadducts (VI), (IX) or (XI).
The Challenge and Responsibility of the L.D.S. Teacher (1965)

This article, adapted from an address to graduates from Brigham Young University in English, details the challenges particular to teachers who are members of The Church of Jesus Christ of Latter-day Saints. The author lists seven attitudes teachers should avoid and lists qualities teachers should strive to develop. These include teaching and caring about the individual, fostering in students an excitement for learning, and working hard. In addition to these qualities, Latter-day Saint teachers as well need to live with integrity and show, through their actions and examples, that religion is not diametrically opposed to philosophy, literature, and science.
Glucose Induces Protein Targeting to Glycogen in Hepatocytes by Fructose 2,6-Bisphosphate-Mediated Recruitment of MondoA to the Promoter (2012)

In the liver, a high glucose concentration activates transcription of genes encoding glucose 6-phosphatase and enzymes for glycolysis and lipogenesis by elevation in phosphorylated intermediates and recruitment of the transcription factor ChREBP (carbohydrate response element binding protein) and its partner, Mlx, to gene promoters. A proposed function for this mechanism is intracellular phosphate homeostasis. In extrahepatic tissues, MondoA, the paralog of ChREBP, partners with Mlx in transcriptional induction by glucose. We tested for glucose induction of regulatory proteins of the glycogenic pathway in hepatocytes and identified the glycogen-targeting proteins, G(L) and PTG (protein targeting to glycogen), as being encoded by Mlx-dependent glucose-inducible genes. PTG induction by glucose was MondoA dependent but ChREBP independent and was enhanced by forced elevation of fructose 2,6-bisphosphate and by additional xylitol-derived metabolites. It was counteracted by selective depletion of fructose 2,6-bisphosphate with a bisphosphatase-active kinase-deficient variant of phosphofructokinase 2/fructosebisphosphatase 2, which prevented translocation of MondoA to the nucleus and recruitment to the PTG promoter. We identify a novel role for MondoA in the liver and demonstrate that elevated fructose 2,6-bisphosphate is essential for recruitment of MondoA to the PTG promoter. Phosphometabolite activation of MondoA and ChREBP and their recruitment to target genes is consistent with a mechanism for gene regulation to maintain intracellular phosphate homeostasis.
3D Reconstruction of Cameras and Structure (2004)

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Sensorfusion Using Spatio-Temporal Aligned Video and Lidar for Improved Vehicle Detection (2006)

This contribution introduces a novel approach to cross-calibrate automotive vision and ranging sensors. The resulting sensor alignment allows the incorporation of multiple sensor data into a detection and tracking framework. Exemplarily, we show how a realtime vehicle detection system, intended for emergency breaking or ACC applications, benefits from the low level fusion of multibeam lidar and vision sensor measurements in discrimination performance and computational complexity
Estimation of DME Thermo-Physical Properties by Molecule Theory (2004)

In this paper, the molecule theory was used to estimate the correlations of DME thermo-physical properties versus temperature. The estimated DME properties include the vapor pressure, the viscosity, the latent heat, the surface tension, the thermal conductivity and the diffusion coefficient. The estimated results give a good precision for non-polar molecule of DME. Moreover, the estimation of DME thermal properties can satisfy the demand for the numerical simulation of DME spray and combustion process. It was proven that the molecule theory can supply a new approach for the estimation of properties for the unknown fuels.
Hybrid inorganic–organic core–shell metal oxide nanoparticles from metal salts (2004)

A microemulsion-based route to amorphous metal oxide core polymer shell nanoparticles for iron, titanium, zinc and zirconium is presented. The first step consists of the basic hydrolysis and condensation of metal salts, using FeCl2·4H2O, TiCl4, ZrO(NO3)2, and ZnBr2 as precursors. The formed particles had diameters between 100 and 640 nm and their surface was modified in a second step with silane coupling agents containing initiating groups for thermally and photochemically induced free radical as well as atom transfer radical polymerizations (ATRP). The thus obtained surface-functionalized inorganic cores were used as multifunctional initiators in grafting from polymerizations of methyl methacrylate (MMA) and styrene as monomers.
Delivering Delivery: Theatricality and the Emasculation of Eloquence (1997)

Abstract: Ever since Aristotle noted in the Rhetoric that, when fashionable, delivery ταύτό ποιήσϵι τῆ ύποκριτικῆ (has “the same effect as acting”; 1404a), classical and medieval rhetorical theorists fulminated against a crowd-pleasing oratory that had devolved into a theatrical spectacle more akin to that provided by the comic “actress” or the “effeminate” male. It cannot be coincidental, however, that, as the fifth rhetorical canon documents the theatricalization of rhetoric, it also offers companion testimony about the so-called emasculation of eloquence. In this essay, I examine the early belief that legal and religious rituals crossed gender lines into effeminacy at they same time that they crossed genre lines into theater. Close analysis suggests that the persistent association between theatrics, bad rhetoric, and effeminacy struck four different targets in a single, well-conceived blow: it marginalized women, homosexuals, bad oratory, and theater by casting certain types of speakers and speech as perverse and disempowered. Delivering delivery today thus entails exposing the ways in which early theorists themselves attempted to deliver it from evil.
Studies on H<SUB>2</SUB>O-based Atomic Layer Deposition of Al<SUB>2</SUB>O<SUB>3</SUB> Dielectric on Pristine Graphene (2012)

Al2O3 films were deposited directly onto the surface of graphene by H2O-based atomic layer deposition (ALD) method, where physically absorbed water molecules acted as oxidant and the growing temperature changed from 60 degrees C to 260 degrees C. The morp
Purification and characterization of the enzymes of fructan biosynthesis in tubers of<i>Helianthus tuberosus</i>‘Colombia’ (1994)

Fructan: fructan fructosyl transferase (FFT), one of the enzymes involved in the synthesis of β-2,1 linked fructose polymers has been purified 205-fold from tubers of Helianthus tuberosus harvested in the accumulation phase. The molecular weight of the native as well as the SDS-denatured protein is approximately 70 kDa. On IEF, the protein was separated into five molecular species with pl values between pH 4.5–5.0. The optimum pH for fructosyl transfer activity was between 5.5–7.0. Temperature optimum was in the range of 25-35° C; the Q10 value between 25 and 5° C was 1.14. FTT catalysed the self-transfer of fructosyl groups with GF2, GF3, GF4 or GF5 as substrate and acceptor. The rate of elf-transfer with both GF2 and GF3 increased linearly with substrate concentration up to 100 mol m−3 and was still not saturated at 600 and 300 mol m−3, respectively. FFT was unable to hydrolyse GF or to catalyse the self-transfer with GF but could mediate the transfer of fructosyl units from inulin on to GF.
Epidermal Growth Factor Binds to a Receptor on <i>Trypanosoma cruzi</i>Amastigotes Inducing Signal Transduction Events and Cell Proliferation (2002)

Host growth factors induce proliferation of Trypanosoma cruzi amastigotes by mechanisms that remain poorly defined. Here we examined human epidermal growth factor (EGF) for its ability to bind to the mammalian multiplicative forms of T. cruzi and to induce growth of the parasites. EGF stimulated incorporation of [3H] thymidine into DNA and growth of amastigotes both in a concentration-dependent manner. Radiolabeled EGF was found to bind to amastigotes in a concentration-dependent and saturable manner but it did not bind to trypomastigotes. Scatchard analysis showed a single class of receptors with a Kd of 0.8 nM and numbering 3.1 x 10(3) per amastigote. Results from internalization experiments provided evidence of receptor-mediated endocytosis of EGF. Northern analysis showed a 3.0-kb transcript for the putative EGF receptor (EGFR) homologue in amastigotes, but not trypomastigotes. Binding of EGF to amastigotes induced signal transduction events. EGF induced "in vitro" kinase activity as determined by gamma-[32P] ATP incorporation into amastigote proteins. EGF also increased protein kinase C activity in a concentration-dependent manner and Mitogen Activated Protein (MAP) kinase activity in a time- and concentration-dependent manner. A specific inhibitor (AG14782) of the EGFR and a MAP kinase inhibitor (PD98059) decreased EGF-dependent T. cruzi MAP kinase activity. These results describe a novel mechanism used by amastigotes to regulate their proliferation mediated by an EGF-dependent signal transduction pathway.
Mangosteen pericarp extract inhibits the formation of pentosidine and ameliorates skin elasticity (2015)

The inhibition of advanced glycation end-products (AGEs) by daily meals is believed to become an effective prevention for lifestyle-related diseases. In the present study, the inhibitory effect of hot water extracts of mangosteen (Garcinia mangostana L.) pericarp (WEM) on the formation of pentosidine, one of AGEs, in vitro and in vivo and the remedial effect on skin conditions were measured. WEM significantly inhibited pentosidine formation during gelatin incubation with ribose. Several compounds purified from WEM, such as garcimangosone D and rhodanthenone B, were identified as inhibitors of pentosidine formation. Oral administration of WEM at 100 mg/day to volunteer subjects for 3 months reduced the serum pentosidine contents. Because obtaining skin biopsies from healthy volunteers is ethically difficult, AGE accumulation in the skin was estimated by a fluorescence detector. The oral administration of WEM significantly reduced the skin autofluorescence intensity, demonstrating that WEM also reduced AGE accumulation in the skin. Furthermore, the elasticity and moisture content of the skin was also improved by WEM. These results demonstrate that intakes of WEM reduces the glycation stress and results in the improvement of skin conditions.
On the power of magic (1987)

This paper considers the efficient evaluation of recursive queries expressed using Horn Clauses. We define sideways information passing formally and show how a query evaluation algorithm may be defined in terms of sideways information passing and control. We then consider a class of information passing strategies which suffices to describe most query evaluation algorithms in the database literature, and show that these strategies may always be implemented by rewriting a given program and evaluating the rewritten program bottom-up. We describe in detail several algorithms for rewriting a program. These algorithms generalize the Counting and Magic Sets algorithms to work with arbitrary programs. Safety and optimality of the algorithms are also considered.
Modification of unsaturated polyester resin by polyurethane prepolymers (2006)

Abstract Unsaturated polyester resins (UPRs) are extensively used by the fiber‐reinforced plastic (FRPs) industry. These resins have the disadvantages of brittleness and poor resistance to crack propagation. In this study, UPRs were chemically modified by reactive blending with polyurethane prepolymers having terminal isocyanate groups. Hybrid networks were formed by copolymerisation of unsaturated polyesters with styrene and simultaneous reaction between terminal hydroxyl groups of unsaturated polyester and isocyanate groups of polyurethane prepolymer. The prepolymers were based on toluene diisocyanate (TDI) and each of hydroxy‐terminated natural rubber (HTNR), hydroxy‐terminated polybutadiene (HTPB), polyethylene glycol (PEG), and castor oil. Properties like tensile strength, toughness, impact resistance, and elongation‐at‐break of the modified UPRs show considerable improvement by this modification. The thermal stability of the copolymer is also marginally better. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 449–456, 2006
An integrated epidemiological and neural net model of the warfarin effect in managed care patients (2017)

Enrichment methodology improves the statistical discrimination of meaningful endpoints when used in a health records-based analysis.
Flexibility of the molecular forms of acetylcholinesterase measured with steady-state and time-correlated fluorescence polarization spectroscopy (1985)

Steady-state and time-correlated fluorescence polarizations have been examined for selected complexes and covalent conjugates of the 11S and (17 + 13)S forms of Torpedo acetylcholinesterase. The 11S form exists as a tetramer of apparently identical subunits, whereas the (17 + 13)S forms contain two or three sets of tetramers disulfide-linked to an elongated collagen-like tail unit. Pyrenebutyl methylphosphonofluoridate and (dansylsulfonamido)pentyl methylphosphonofluoridate were conjugated at the active center serine whereas propidium was employed as a fluorescent ligand for the spatially removed peripheral anionic site. Steady-state polarization of the pyrenebutyl conjugates indicates rotational correlation times of approximately 400 ns for the 11S species and greater than 1100 ns for the (17 + 13)S species. Hence, the tail unit severely restricts rotational motion of the catalytic subunits. Time-correlated fluorescence polarization analysis of the 11S species indicates multiple rotational correlation times. Anisotropy decay of the propidium complex (tau = 6 ns) occurs in exponential manner with a rotational correlation time of approximately 150 ns, while covalent adducts at the active center exhibit rotational correlation times greater than or equal to 300 ns. Anisotropy decay of the (dansylsulfonamido)pentyl conjugate (tau = 16 ns) appears exponential with a correlation time of approximately 320 ns, whereas decay of the pyrenebutyl conjugate (tau = 100 ns) is described by two correlation times, phi S = 18 ns and phi L = 320 ns, of small (15%) and large (85%) amplitudes, respectively. Two limiting models have been considered to explain the results.(ABSTRACT TRUNCATED AT 250 WORDS)
恶搞来袭，杜南“很忙”——聚焦网络“恶搞杜甫”事件 (2012)

“杜甫，男，唐朝著名诗人，汉族，字子美，世称杜少陵、杜工部、杜拾遗等，自号少陵野老，生于公元712年，卒于公元770年，忙于公元2012年。”这是最近盛行在网络上的对杜甫生平的最新介绍。
Effects of fatigue and training on sarcoplasmic reticulum Ca<sup>2+</sup>regulation in human skeletal muscle (2002)

Little is known about fatigue and training effects on sarcoplasmic reticulum (SR) function in human muscle, and we therefore investigated this in eight untrained controls (UT), eight endurance-trained (ET), and eight resistance-trained athletes (RT). Muscle biopsies (vastus lateralis) taken at rest and after 50 maximal quadriceps contractions (180 degrees/s, 0.5 Hz) were analyzed for fiber composition, metabolites and maximal SR Ca(2+) release, Ca(2+) uptake, and Ca(2+)-ATPase activity. Fatigue reduced (P < 0.05) Ca(2+) release (42.1 +/- 3.8%, 43.4 +/- 3.9%, 31.3 +/- 6.1%), Ca(2+) uptake (43.0 +/- 5.2%, 34.1 +/- 4.6%, 28.4 +/- 2.8%), and Ca(2+)-ATPase activity (38.6 +/- 4.2%, 48.5 +/- 5.7%, 29.6 +/- 5.0%), in UT, RT, and ET, respectively. These decreases were correlated with fatigability and with type II fiber proportion (P < 0.05). Resting SR measures were correlated with type II proportion (r > or = 0.51, P < 0.05). ET had lower resting Ca(2+) release, Ca(2+) uptake, and Ca(2+)-ATPase (P < 0.05) than UT and RT (P < 0.05), probably because of their lower type II proportion; only minor effects were found in RT. Thus SR function is markedly depressed with fatigue in controls and in athletes, is dependent on fiber type, and appears to be minimally affected by chronic training status.
Identification of TRIO‐GEFD1 chemical inhibitors using the yeast exchange assay (2006)

The development of RhoGEF inhibitors appears as a valuable tool for the study of Rho GTPase signalling pathways. The yeast exchange assay adaptation we present here is suitable to screen for chemical or peptide libraries and identify candidate inhibitors.
Det2Seg: A Two-Stage Approach for Road Object Segmentation from 3D Point Clouds (2019)

Object segmentation from 3D point clouds is an important topic in real-world applications. However, due to the sparsity and irregularity of point clouds, instance segmentation often suffers unsatisfying performance in various scenes such as autonomous driving. There are two main difficulties. First, in a wild scene, background noise often occupies a majority of the entire point set. Second, small-scale objects are often difficult to be recognized due to larger uncertainty. In this paper, we propose Det2Seg, a two-stage approach which alleviates the above issues towards more accurate instance segmentation of road-objects. In the first stage, we adopt Pointpillars [1] to detect the regions-of- interest that can localize and classify objects in a coarse level; in the second stage, we extract points from the detected regions into pillars, encode them into a new data format and feed it into a 2D convolutional neural network to perform fine-grained, domain-specific instance segmentation. We evaluate our approach on raw LiDAR (Light Detection And Ranging) data from the KITTI dataset [2]. The experimental results show that our approach largely outperforms the prior researches. In particular, our approach stands out for its significant ability on recognizing and segmenting small-scale objects, i.e., an improvement of over 20% in terms of Intersection over Union (IoU), beyond state-of- the-arts, is obtained for the cyclist class.
The Use of low-dose Histamine Therapy in Otolaryngology (1999)

Low-dose histamine therapy has been prescribed by otolaryngologists primarily to treat Bell's palsy, vestibular disorders, vascular headache, Ménière's attacks, and urticaria vasculitis. The scientific explanations for the usefulness of this once-empiric treatment are becoming more apparent. Two methods of establishing the appropriate dosages have emerged: the empiric, optimum-dosage approach and the objective, endpoint-titration approach. In this article, the author describes and recommends the latter approach. The author also reports on a 100-patient retrospective clinical study that revealed that the objective, endpoint-titration approach was effective in treating 80% of patients. In light of such success and the ease and economy of this treatment, low-dose histamine therapy appears to be a valuable clinical tool.