gebhart/patrepeval huggingface dataset:
Protease-Activated Receptor-2 Activating Peptide SLIGRL Inhibits Bacterial Lipopolysaccharide-Induced Recruitment of Polymorphonuclear Leukocytes into the Airways of Mice (2002)

Protease-activated receptor-2 (PAR2) acts as a receptor for trypsin and trypsin-like enzymes. The role of this receptor in airway inflammation is uncertain. In this study we assessed the effect of activation of PAR2 following intranasal administration of the peptide activator of PAR2, SLIGRL, over 72 h in mice. The extent of immune cell infiltration into the airways and activities of matrix metalloprotease-2 (MMP-2) and MMP-9 were assessed in bronchoalveolar lavage (BAL) by differential cell counts and gelatin zymography, respectively. SLIGRL did not cause a change in the number or types of cells retrieved in BAL at any time point and did not alter the levels of MMP-2 and MMP-9 present in BAL. In contrast, similar intranasal administration of bacterial lipopolysaccharide (LPS) caused a large influx of neutrophilic polymorphonuclear leukocytes, which was associated with increased MMP-2 at 3 h only and MMP-9 activity from 3-72 h. Simultaneous administration of SLIGRL and LPS transiently potentiated increased MMP-9 activity at 3 h but markedly inhibited neutrophil influx and elevated MMP-2 activity at 3 h. These findings suggest that PAR2 agonists may be useful therapeutic molecules in pulmonary inflammatory diseases.
Differential activities of bacteriophage depolymerase on bacterial polysaccharide: binding is essential but degradation is inhibitory in phage infection of K1-defective Escherichia coli (1992)

Host range mutants were derived from bacteriophages PK1A and PK1E specific for the K1 polysialic acid capsule of Escherichia coli. The mutants were selected for their ability to infect E. coli bacteria with a low level of the K1 capsule. A specific loss of the cleaving activity of the phage endosialidase was observed in all the mutants, while the ability to bind specifically to the polysialic acid capsule was retained. The results indicate that the polysaccharide-binding activity of the bacteriophage enzyme is essential for the infection process. The cleaving activity, in contrast, is required for the penetration of the dense polysaccharide of wild-type bacteria but is inhibitory in the infection of bacteria with a sparse capsular polysaccharide.
Implementation of an all-digital DS-CDMA receiver-chip synchronization for an orthogonally coded cellular CDMA system (2003)

The authors propose an all-digital code tracking scheme for a bandlimited cellular direct-sequence code-division-multiple-access (DS-CDMA) system, which uses bandlimited Nyquist chip pulse shaping and orthogonal coding. The code tracking loop has a decision-directed noncoherent structure to handle the orthogonally coded DS-CDMA signals. The performance of the tracking loop is derived analytically.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Study on the Lighting Environment Improvement of the VDT Workspaces in Computer Room (1997)

The purpose of this study was to improve the lighting environment of the VDT workspaces in computer laboratory. This study was carried out through the field measurements on the computer laboratory and a survey using the questionnaire about the VDT workspaces. The major findings of the study were as follows: (1) It was unbalanced among the illumination, luminance, and color distributions in the the VDT workspaces. (2) The difference of brightness between the CRT screen and its surroundings have presented on the whole unbalanced. (3) It was proved that the artificial light source of the ceiling lighting should use the parabolic louver luminaire limiting the radiant luminance.
QELAR: A Machine-Learning-Based Adaptive Routing Protocol for Energy-Efficient and Lifetime-Extended Underwater Sensor Networks (2010)

Underwater sensor network (UWSN) has emerged in recent years as a promising networking technique for various aquatic applications. Due to specific characteristics of UWSNs, such as high latency, low bandwidth, and high energy consumption, it is challenging to build networking protocols for UWSNs. In this paper, we focus on addressing the routing issue in UWSNs. We propose an adaptive, energy-efficient, and lifetime-aware routing protocol based on reinforcement learning, QELAR. Our protocol assumes generic MAC protocols and aims at prolonging the lifetime of networks by making residual energy of sensor nodes more evenly distributed. The residual energy of each node as well as the energy distribution among a group of nodes is factored in throughout the routing process to calculate the reward function, which aids in selecting the adequate forwarders for packets. We have performed extensive simulations of the proposed protocol on the Aqua-sim platform and compared with one existing routing protocol (VBF) in terms of packet delivery rate, energy efficiency, latency, and lifetime. The results show that QELAR yields 20 percent longer lifetime on average than VBF.
Importance of Glutamate 279 for the Coenzyme Binding of Human Glutamate Dehydrogenase (2002)

Although the structure of glutamate dehydrogenase (GDH) has been reported from various sources including mammalian GDH, there are conflicting views regarding the location and mechanism of actions of the coenzyme binding. We have expanded these speculations by photoaffinity labeling and cassette mutagenesis. Photoaffinity labeling with a specific probe, [(32)P]nicotinamide 2-azidoadenosine dinucleotide, was used to identify the NAD(+) binding site within human GDH encoded by the synthetic human GDH gene and expressed in Escherichia coli as a soluble protein. Photolabel-containing peptides generated with trypsin were isolated by immobilized boronate affinity chromatography. Photolabeling of these peptides was most effectively prevented by the presence of NAD(+) during photolysis, demonstrating a selectivity of the photoprobe for the NAD(+) binding site. Amino acid sequencing and compositional analysis identified Glu(279) as the site of photoinsertion into human GDH, suggesting that Glu(279) is located at or near the NAD(+) binding site. The importance of the Glu(279) residue in the binding of NAD(+) was further examined by cassette mutagenesis with mutant enzymes containing Arg, Gly, Leu, Met, or Tyr at position 279. The mutagenesis at Glu(279) has no effects on the expression or stability of the different mutants. The K(m) values for NAD(+) were 10-14-fold greater for the mutant GDHs than for wild-type GDH, whereas the V(max) values were similar for wild-type and mutant GDHs. The efficiency (k(cat)/K(m)) of the mutant GDH was reduced up to 18-fold. The decreased efficiency of the mutants results from the increase in K(m) values for NAD(+). In contrast to the K(m) values for NAD(+), wild-type and mutant GDHs show similar K(m) values for glutamate, indicating that substitution at position 279 had no appreciable effect on the affinity of enzyme for glutamate. There were no differences in sensitivities to ADP activation and GTP inhibition between wild-type and mutant GDH, suggesting that Glu(279) is not directly involved in allosteric regulation. The results with photoaffinity labeling and cassette mutagenesis studies suggest that Glu(279) plays an important role for efficient binding of NAD(+) to human GDH.
[Synthesis of some compounds related to tanshinquinone]. (1985)

The lipolytic components of Dan Shen (Salvia miltiorrhiza Bunge) were elucidated as a group of diterpene quinones such as tanshinone Ⅰ, tanshinone ⅡA and cryptotanshinone. These compounds were found to increase the resistance of mice to hypoxia in pharmacological tests. Since the activity of these lipolytic components may be due to the presence of the quinone structure, some derivatives with latent quinones have been synthesized. Pharmacological investigations revealed that the biological activity was comparatively weakened.Furthermore, the water soluble sodium sulphonate derivative of tanshinone ⅡA was more active against coronary disease in clinical trial with some side effects. A series of water soluble Mannich bases derived from tanshinones were also prepared. Preliminary pharmacological examination showed that one of them Xa exhibited high bacteriostatic activity against staphylococcus aureus species 18.
A Feasible Approach to Evaluate the Relative Reactivity of NHS-Ester Activated Group with Primary Amine-Derivatized DNA Analogue and Non-Derivatized Impurity (2015)

Synthetic DNA analogues with improved stability are widely used in life science. The 3'and/or 5' equivalent terminuses are often derivatized by attaching an active group for further modification, but a certain amount of non-derivatized impurity often remains. It is important to know to what extent the impurity would influence further modification. The reaction of an NHS ester with primary amine is one of the most widely used options to modify DNA analogues. In this short communication, a 3'-(NH2-biotin)-derivatized morpholino DNA analogue (MORF) was utilized as the model derivatized DNA analogue. Inclusion of a biotin concomitant with the primary amine at the 3'-terminus allows for the use of streptavidin to discriminate between the products from the derivatized MORF and non-derivatized MORF impurity. To detect the MORF reaction with NHS ester, S-acetyl NHS-MAG3 was conjugated to the DNA analogue for labeling with (99m)Tc, a widely used nuclide in the clinic. It was found that the non-derivatized MORF also reacted with the S-acetyl NHS-MAG3. Radiolabeling of the product yielded an equally high labeling efficiency. Nevertheless, streptavidin binding indicated that under the conditions of this investigation, the non-derivatized MORF was five times less reactive than the amine-derivatized MORF.
High-efficiency supercontinuum generation in solid thin plates at 01  TW level (2017)

Supercontinuum generation in a solid-state medium was investigated experimentally. A continuum covering 460 to 950 nm was obtained when 0.8 mJ/30 fs Ti:sapphire laser pulses were applied to seven thin fused silica plates at a 1 kHz repetition rate. The primary processes responsible for spectral broadening were self-phase modulation (SPM) and self-steepening, while SPM and self-focusing were balanced to optimize the spectral broadening and suppress the multiphoton process. The output was compressed to a 5.4 fs and a 0.68 mJ pulse, corresponding to two optical cycles and 0.13 TW of peak power.
Efficacy and Rate of Release of EPTC and Butylate from Starch Encapsulated Formulations Under Greenhouse Conditions (1978)

Four starch-encapsulated formulations of EPTC (S -ethyl dipropylthiocarbamate) and of butylate (S -ethyl diisobutylthiocarbamate) were prepared and evaluated by comparison with their respective emulsifiable concentrate formulations for their slow-release capabilities and efficacies. Chemical and biological evaluation indicated that difference in controlled-release could be achieved by the selection of the starch xanthate and oxidant used in the formualtion process. EPTC and butylate released slower when formulated as starch-encapsulated granules than when formulated as emulsifiable concentrates under soil conditions that favored rapid release. The initial release was adequate for weed control and slow enough for desired residual activity. Repeated seeding and harvesting the treated soils and bioassays of treated soils generally produced release rate anticipated from short term dry and wet chemical tests.
Efficient location area planning for personal communication systems (2006)

A central problem in personal communication systems is to optimize bandwidth usage, while providing Quality of Service (QoS) guarantees to mobile users. Network mobility management, and in particular, location management, consumes a significant portion of bandwidth, which is a necessary overhead for supporting mobile users. We focus our efforts on minimizing this overhead. Unlike previous works, we concentrate on optimizing existing schemes, and so the algorithms we present are easily incorporated into current networks. We present the first polynomial time approximation algorithms for minimum bandwidth location management. In planar graphs, our algorithm provably generates a solution that uses no more than a constant factor more bandwidth than the optimal solution. In general graphs, our algorithm provably generates a solution that uses just a factor O (log n) more bandwidth than optimal where n is the number of base stations in the network. We show that, in practice, our algorithm produces near-optimal results and outperforms other schemes that are described in the literature. For the important case of the line graph, we present a polynomial-time optimal algorithm. Finally, we illustrate that our algorithm can also be used for optimizing the handoff mechanism.
Genetic Theorizing and Schizophrenia (1973)

Schizophrenia research embodies a microcosm of the vexing problems that confront the behavioural sciences, but particularly those disciplines concerned with psychopathology. We are ignorant of the means to prevent schizophrenia because we continue to be ignorant about its aetiology. Despite recognizable descriptions of the syndrome in ancient Hindu treatises ( c. 1400 b.c.) and 76 years after its designation as dementia praecox by Kraepelin (1896), we are still grappling with such basic issues as when and how to diagnose Eugen Bleuler's (1911) ‘group of schizophrenias' (cf. Katz, Cole, and Barton, 1968). Despite brilliant advances in molecular biology, neurochemistry, and brain-behaviour phenomena generally, we cannot pinpoint any necessary biological defect in all or most schizophrenics. Despite selfless expenditures of time and energy by gifted psychotherapists and sophisticated social science efforts, we cannot specify any necessary life experience, either at the level of the family or of a culture, common to all or most schizophrenics. While there is obvious merit in casting the problem in an interactionist framework, aetiology still defies an easy solution because we must then isolate what element(s) in the genotype interact with what element(s) in the internal and/or external environment (as well as when and how) to produce the phenotype we recognize as a schizophrenic one.
Monitoring of Honeydew Excretion in the Field as a Method of Sampling Illinoia liriodendri (Homoptera: Aphididae) Infesting Liriodendron tulipifera (1987)

Honeydew excreted by the tuliptree aphid, Illinoia liriodendri (Monell), was monitored in the field using yellow water-sensitive papers on tuliptrees (Liriodendron tulipifera L.) at two sites in Berkeley, Calif. Significant correlations were found between aphid numbers and honeydew (droplets per hour per square centimeter) except at relatively low densities (approximately four aphids per leaf). Honeydew excretion rate was positively correlated with temperature. The highest rate occurred from 1100 to 1500 hours and the lowest from 1900 to 0700 hours. Monitoring honeydew required 30% less sampling time than counting insects. An efficient method of monitoring tuliptree aphid honeydew will permit more precise timing of spot treatments and could be adapted to monitor other honeydew-excreting insects in the field.
Raman Spectra of Biological Samples: A Study of Preprocessing Methods (2006)

In this study preprocessing of Raman spectra of different biological samples has been studied, and their effect on the ability to extract robust and quantitative information has been evaluated. Four data sets of Raman spectra were chosen in order to cover different aspects of biological Raman spectra, and the samples constituted salmon oils, juice samples, salmon meat, and mixtures of fat, protein, and water. A range of frequently used preprocessing methods, as well as combinations of different methods, was evaluated. Different aspects of regression results obtained from partial least squares regression (PLSR) were used as indicators for comparing the effect of different preprocessing methods. The results, as expected, suggest that baseline correction methods should be performed in advance of normalization methods. By performing total intensity normalization after adequate baseline correction, robust calibration models were obtained for all data sets. Combination methods like standard normal variate (SNV), multiplicative signal correction (MSC), and extended multiplicative signal correction (EMSC) in their basic form were not able to handle the baseline features present in several of the data sets, and these methods thus provide no additional benefits compared to the approach of baseline correction in advance of total intensity normalization. EMSC provides additional possibilities that require further investigation.
The design and testing of the super fiber heat pipes for electronics cooling applications (2002)

Cooling of electronics is one of the major fields of application for heat pipes (3-9-mm outside diameter) with a worldwide demand exceeding one million per month. The high heat fluxes associated with electronics cooling require heat pipes with high maximum heat transfer at any inclination, and therefore improved wick structures are needed. In particular, the operation at top heat mode (vertical orientation) is required by most notebook manufacturers with a decrease of 30%-50% of the thermal resistance over conventional systems. A new wick structure has been developed so the capillary channels are increased with small effects on the heat pipe permeability. Using this new design criterion, (which balances the permeability and capillary needs), super fiber bundle heat pipes have been developed. The diameter of the wire used in the fabrication varied from 0.05 to 0.1 mm and the maximum input power was 16 W. It was found that the vapor space/liquid space ratio is an important parameter for this type of heat pipe. The test results show that the thermal resistance of the heat pipes is a strong function of the orientation. We have fabricated heat pipes with two to five times lower thermal resistance than previous conventional heat pipes (for the top heat mode operation). A comparison with other types of wick structures is also presented. Thermal resistances as low as 0.5/spl deg/C/W (top heat mode) and 0.2/spl deg/C/W (horizontal operation) have been observed. The application to electronics cooling it has been successful, especially in notebook computers and telecommunications applications.
THE CLINICAL EFFECTS OF INTENSIVE, SPECIFIC EXERCISE ON CHRONIC LOW BACK PAIN: A CONTROLLED STUDY OF 895 CONSECUTIVE PATIENTS WITH 1-YEAR FOLLOW UP (1995)

Eight hundred ninety-five consecutive chronic low back pain patients were evaluated. Six hundred twenty-seven completed the program. One hundred sixty-one began, but dropped out, and 107 were recommended for treatment but did not undergo treatment for various reasons. Average duration of symptoms prior to evaluation was 26 months. Forty-seven percent of patients were workers' compensation patients. The primary treatment was intensive, specific exercise using firm pelvic stabilization to isolate and rehabilitate the lumbar spine musculature. Patients were encouraged to work hard to achieve specific goals. Seventy-six percent of patients completing the program had excellent or good results. At 1-year follow up 94% of patients with good or excellent results reported maintaining their improvement. Results in the control group were significantly poorer in all areas surveyed except employment.
Chemical transfection of dye‐conjugated microRNA precursors for microRNA functional analysis of M2 macrophages (2011)

MicroRNAs (miRNAs) are short noncoding ribonucleic acids known to affect gene expression at the translational level and there is mounting evidence that miRNAs play a role in the function of tumor-associated macrophages (TAMs). To aid the functional analyses of miRNAs in an in-vitro model of TAMs known as M2 macrophages, a transfection method to introduce artificial miRNA constructs or miRNA molecules into primary human monocytes is needed. Unlike differentiated macrophages or dendritic cells, undifferentiated primary human monocytes have been known to show resistance to lentiviral transduction. To circumvent this challenge, other techniques such as electroporation and chemical transfection have been used in other applications to deliver small gene constructs into human monocytes. To date, no studies have compared these two methods objectively to evaluate their suitability in the miRNA functional analysis of M2 macrophages. Of the methods tested, the electroporation of miRNA-construct containing plasmids and the chemical transfection of miRNA precursor molecules are the most efficient approaches. The use of a silencer siRNA labeling kit (Ambion) to conjugate Cy 3 fluorescence dyes to the precursor molecules allowed the isolation of successfully transfected cells with fluorescence-activated cell sorting. The chemical transfection of these dye-conjugated miRNA precursors yield an efficiency of 37.5 ± 0.6% and a cell viability of 74 ± 1%. RNA purified from the isolated cells demonstrated good quality, and was fit for subsequent mRNA expression qPCR analysis. While electroporation of plasmids containing miRNA constructs yield transfection efficiencies comparable to chemical transfection of miRNA precursors, these electroporated primary monocytes seemed to have lost their potential for differentiation. Among the most common methods of transfection, the chemical transfection of dye-conjugated miRNA precursors was determined to be the best-suited approach for the functional analysis of M2 macrophages.
Interaction between replication protein A and p53 is disrupted after UV damage in a DNA repair-dependent manner (1997)

Replication protein A (RPA) is required for both DNA replication and nucleotide excision repair. Previous studies have shown that RPA interacts with the tumor suppressor p53. Herein, we have mapped a 20-amino acid region in the N-terminal part of p53 that is essential for its binding to RPA. This region is distinct from the minimal activation domain of p53 previously identified. We also demonstrate that UV radiation of cells greatly reduces the ability of RPA to bind to p53. Interestingly, damage-induced hyperphosphorylated RPA does not associate with p53. Furthermore, down-regulation of the RPA/p53 interaction is dependent upon the capability of cells to perform global genome repair. On the basis of these data, we propose that RPA may participate in the coordination of DNA repair with the p53-dependent checkpoint control by sensing UV damage and releasing p53 to activate its downstream targets.
The Accessibility of the Bone Induction Principle in Surface-Declacified Bone Implants (1967)

From the Bone Research Laboratory, U.C.L.A. Medical School, Division of Orthopedics, Department of Surgery, Los Angeles, Calif. 90024.