gebhart/patrepeval huggingface dataset:
Neuroendocrine Control of Thymic Hormonal Production. I. Prolactin Stimulates<i>in Vivo</i>and<i>in Vitro</i>the Production of Thymulin by Human and Murine Thymic Epithelial Cells (1989)

The thymic epithelium is responsible for the secretion of thymic peptides, which intervene in some steps of intra- and extrathymic T cell differentiation. Recent data suggest that thymic hormone secretion is modulated by the neuroendocrine network, comprising thyroid, adrenals, and gonads. However, the role of the pituitary gland in this regulation is still poorly understood. In the present paper we studied the in vivo and in vitro influences of PRL on the secretion of thymulin, one of the chemically defined thymic hormones, by thymic epithelial cells (TEC). When injected daily (20-100 micrograms/20 g) in young or old C57BL/6 mice, PRL induced a specific increase in thymulin synthesis and secretion, respectively, measured by the number of thymulin-producing cells in the thymus and the peripheral levels of the hormone. This stimulation was dose dependent and reversible after the end of treatment. Similar findings have been made in animals with pituitary dwarfism, known to have low levels of circulating thymulin. This stimulatory effect was also observed in primary cultures of human and mouse TEC when PRL (10(-7) to 10(-8) M) was applied to culture supernatants, thus suggesting that PRL could act directly on TEC. In addition, we induced in vivo experimental hypoprolactinemia, treating mice with bromocriptine, a dopamine receptor agonist that inhibits pituitary PRL secretion. Bromocriptine treatment (100-200 micrograms/20 g) yielded a significant decrease in thymulin secretion that could be reversed by coincident treatment with PRL. In the light of previous observations that bovine GH can also increase thymulin production in aged dogs, we performed a series of experiments in vitro to evaluate whether GH has a direct effect on TEC. We observed that only human GH preparations that are known to have a PRL-like effect were efficient in stimulating thymulin biosynthesis and release into the culture supernatants. The effects of PRL on TEC were not restricted to thymic hormone production. We observed that TEC proliferation, as well as the numbers of a TEC subset defined by the expression of cytokeratins 3 and 10, could also be increased by PRL treatment. All these findings show that the pituitary gland directly affects TEC in terms of cytoskeletal and secretory protein expression as well as cell cycle.
Characterization of Petroleum Asphaltenes by Size Exclusion Chromatography, UV-fluorescence and Mass Spectrometry (2007)

Abstract The molecular weight of petroleum asphaltenes remains the subject of debate. Our previous work using size exclusion chromatography with 1-methyl-2-pyrrolidinone (NMP) as eluent, combined with mass spectrometry and fluorescence spectroscopy has indicated molecular masses up to 40,000 u. The present work investigates the asphaltene fraction of Kuwaiti crude oil. The asphaltene was separated into several NMP-soluble fractions and an insoluble residue. The evidence from size exclusion chromatography (SEC) and UV-fluorescence spectroscopy (UV-F) shows that the molecular size range and the size of the aromatic chromophores increased with increasing insolubility in NMP. A steady increase in the sizes of the range of chromophores was shown by UV-fluorescence in going from the maltene fraction, via the asphaltene sample to the NMP-insoluble residue, using chloroform as solvent. Laser-desorption mass spectra showed very wide mass ranges for the whole asphaltene and the NMP-insoluble residue of asphaltenes, with a signal up to m/z 200,000 and slightly more ion intensity at high masses for the residue compared to that from the asphaltene.
Expeditious Method of Urethrovesical Junction Determination in Retropubic Colposuspension with Intraballoon Illumination of Foley Catheter (2003)

Intraballoon illumination for determination of UVJ is simple, safe, effective and precludes an invasive approach such as cystoscopy and cystotomy.
T-LESS: An RGB-D Dataset for 6D Pose Estimation of Texture-Less Objects (2017)

We introduce T-LESS, a new public dataset for estimating the 6D pose, i.e. translation and rotation, of texture-less rigid objects. The dataset features thirty industry-relevant objects with no significant texture and no discriminative color or reflectance properties. The objects exhibit symmetries and mutual similarities in shape and/or size. Compared to other datasets, a unique property is that some of the objects are parts of others. The dataset includes training and test images that were captured with three synchronized sensors, specifically a structured-light and a time-of-flight RGB-D sensor and a high-resolution RGB camera. There are approximately 39K training and 10K test images from each sensor. Additionally, two types of 3D models are provided for each object, i.e. a manually created CAD model and a semi-automatically reconstructed one. Training images depict individual objects against a black background. Test images originate from twenty test scenes having varying complexity, which increases from simple scenes with several isolated objects to very challenging ones with multiple instances of several objects and with a high amount of clutter and occlusion. The images were captured from a systematically sampled view sphere around the object/scene, and are annotated with accurate ground truth 6D poses of all modeled objects. Initial evaluation results indicate that the state of the art in 6D object pose estimation has ample room for improvement, especially in difficult cases with significant occlusion. The T-LESS dataset is available online at cmp:felk:cvut:cz/t-less.
Tethered IP3. Synthesis and biochemical applications of the 1-O-(3-aminopropyl) ester of inositol (1,4,5)-trisphosphate (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTethered IP3. Synthesis and biochemical applications of the 1-O-(3-aminopropyl) ester of inositol (1,4,5)-trisphosphateGlenn D. Prestwich, James F. Marecek, Robert J. Mourey, Anne B. Theibert, Christopher D. Ferris, Sonye K. Danoff, and Solomon H. SnyderCite this: J. Am. Chem. Soc. 1991, 113, 5, 1822–1825Publication Date (Print):February 1, 1991Publication History Published online1 May 2002Published inissue 1 February 1991https://pubs.acs.org/doi/10.1021/ja00005a055https://doi.org/10.1021/ja00005a055research-articleACS PublicationsRequest reuse permissionsArticle Views478Altmetric-Citations62LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ring Interferometric Magnetic Field Optical Sensor Using a Garnet Single Crystal (2010)

A magnetic-field sensor based on reflection-ring interferometry is proposed to achieve a probing scheme with a polarization maintaining fiber (PMF). We used Tb0.19Y0.81Fe5O12 ((TbY)IG) crystals with a striped magnetic domain structure as a Faraday element to enable both parallel and perpendicular fields to the propagating direction of the optical wave to be measured. A linear magnetization curve without hysteresis and a linear Faraday effect were expected to be obtained as the magnetization process of rotation occurred in each striped magnetic domain. This assumption was confirmed in an experiment. Experimental results such as sensitivity and SNR were well explained by this model. The obtained sensor sensitivity was 1.08 × 10-3 %/(A/m), and good linearity of sensor output was obtained over a wide dynamic range between 1 A/m to 104 A/m.
Aflatoxin in maize (1992)

Abstract Aflatoxin B1 is a potent hepatacarcinogen that occurs in corn worldwide. The aflatoxin‐producing fungus Aspergillus flavus can grow and produce aflatoxin on corn preharvest and in storage. Within the U.S., aflatoxin contamination of preharvest corn has been reported in 23 states, and contamination is chronic in the southeastern U.S. where hot, drought conditions often favor the fungus and the production of aflatoxin. Management practices have been developed to reduce aflatoxin contamination, but in years when environmental conditions are extremely favorable for the fungus, no control strategy is effective. Resistance to aflatoxin accumulation appears to be heritable, but no commercial hybrids are available with adequate resistance. This review covers the epidemiology of A. flavus in preharvest corn from the infection process to the factors that influence aflatoxin formation. Also discussed are the problems associated with the development of resistant varieties and new strategies that are being developed for control of aflatoxin contamination. Key words: aflatoxin Aspergillus flavus maizeplant resistancepolyketidecarcinogen
3D Touchless Fingerprints: Compatibility with Legacy Rolled Images (2006)

Fingerprints are traditionally captured based on contact of the finger on paper or a platen surface. This often results in partial or degraded images due to improper finger placement, skin deformation, slippage and smearing, or sensor noise from wear and tear of surface coatings. A new generation of touchless live scan devices that generate 3D representation of fingerprints is appearing in the market. This new sensing technology addresses many of the problems stated above. However, 3D touchless fingerprint images need to be compatible with the legacy rolled images used in automated fingerprint identification systems (AFIS). In order to solve this interoperability issue, we propose an unwrapping algorithm that unfolds the 3D fingerprint in such a way that it resembles the effect of virtually rolling the 3D finger on a 2D plane. Our preliminary experiments show promising results in obtaining touchless fingerprint images that are of high quality and at the same time compatible with legacy rolled fingerprint images.
Nosocomial and community-acquired infections in malnourished children. (1988)

A total of 206 community-acquired and 73 nosocomial infections in 50 malnourished Jamaican children were studied prospectively. Predominant community-acquired infections in the 50 children, included gastroenteritis (68%), otitis media (60%), rhinopharyngitis (60%), oral candidiasis (46%), skin infections (40%), pneumonia (28%), bacteraemia (24%) and bacteriuria (18%). The most frequent nosocomial infections were rhinopharyngitis (34%), lower respiratory tract infections (24%) and septicaemia (18%). In those infections where an aetiological agent was identified, Giardia lamblia was the commonest enteric pathogen, Staphylococcus epidermidis, the most frequent blood culture isolate and Klebsiella sp. were recovered from the majority of urines. The lack of clinical signs and symptoms and atypical clinical presentation in some infected malnourished children were attributed to impairment of the acute inflammatory response. Diagnosis of infection in these children required a high index of suspicion and a comprehensive screening system. Nasal, throat and axilla swabs taken on admission revealed significant colonization with coliforms and pneumococcus; however, these swabs were not useful as indicators of potentially infective organisms. Four of the 50 children died and two of these deaths were attributed to infection.
The PDZ protein Tip-1 is a gain of function target of the HPV16 E6 oncoprotein (2004)

Previous work has indicated that the PDZ domain Tax interacting protein 1 (Tip-1) is a target of the HTLV1 Tax protein and is a potential RhoA effector. We have used the yeast two-hybrid system to show that Tip-1 also interacts with the HPV16 E6 protein. This interaction was confirmed by co-immunoprecipitation from E6 expressing C33A cervical carcinoma cells (C33A-E6) which showed that Tip-1 was not degraded by interaction with the HPV16 E6 oncoprotein. During routine passage we observed that C33A-E6 had a less compact morphology and were less adherent than control vector transfected cells C33A-V cells - a known effect of GTP-RhoA. Comparison of C33A-E6 to C33A-V demonstrated that E6 expressing cells had higher levels of phosphorylated myosin light chains (MLC) and increased cell motility, which was inhibited by antisense silencing of Tip-1 expression and by the RhoA kinase (ROCK) inhibitor Y27632. Both C33A-E6 and C33A-V cells were shown to express GTP activated RhoA. Since ROCKs can be activated by GTP RhoA these data indicate that E6 may increase cell motility by augmenting GTP RhoA mediated activation of ROCKs and that this is dependent on the expression of the Tip-1 protein.
Qimeng de pipan yu jiushu 啓蒙的批判與救贖 (Critique et rédemption des Lumières) (2006)

National audience
Cloning and Characterization of a Gene Encoding the Major Surface Protein of the Bacterial Endosymbiont<i>Wolbachia pipientis</i> (1998)

The maternally inherited intracellular symbiont Wolbachia pipientis is well known for inducing a variety of reproductive abnormalities in the diverse arthropod hosts it infects. It has been implicated in causing cytoplasmic incompatibility, parthenogenesis, and the feminization of genetic males in different hosts. The molecular mechanisms by which this fastidious intracellular bacterium causes these reproductive and developmental abnormalities have not yet been determined. In this paper, we report on (i) the purification of one of the most abundantly expressed Wolbachia proteins from infected Drosophila eggs and (ii) the subsequent cloning and characterization of the gene (wsp) that encodes it. The functionality of the wsp promoter region was also successfully tested in Escherichia coli. Comparison of sequences of this gene from different strains of Wolbachia revealed a high level of variability. This sequence variation correlated with the ability of certain Wolbachia strains to induce or rescue the cytoplasmic incompatibility phenotype in infected insects. As such, this gene will be a very useful tool for Wolbachia strain typing and phylogenetic analysis, as well as understanding the molecular basis of the interaction of Wolbachia with its host.
Endovascular stents: Preliminary clinical results and future developments (1993)

At present, there is an exponential use of new interventional techniques whose proper role and value have not yet been defined. The intracoronary stent is just one example. There is no doubt that stents can be implanted with a high technical success rate associated with highly predictable immediate angiographic results and that they appear to be superior to all other interventional techniques. However, the intrinsic thrombogenicity of all devices currently available for clinical use warrants a vigorous anticoagulation, exposing the patient either to the risk of (sub)acute stent thrombosis or to the risk of hemorrhage and vascular complications. It remains to be determined whether stent implantation will reduce the incidence of restenosis and whether this results in an improved long-term event and symptom-free survival. Experimental studies indicate that the thrombogenic nature of stents may be controlled by coating the struts with endothelial cells or polymers. With respect to restenosis, it is evident that as long as mechanical injury is applied to the vessel wall, the vessel wall will respond with neointimal thickening. The intracoronary stent has the potential to control this tissue response by serving as a carrier for local antiproliferative drug delivery or eventually for genetic manipulation. The intensive research which is now going on in combination with experimental animal data, human postmortem pathologic observations, and angiographic studies is yielding clear insights and future directions to address these issues.
Tailoring reveals information requirements: the case of anaesthesia alarms (2004)

We discuss the phenomenon of system tailoring in the context of data from an observational study of anaesthesia. We found that anaesthetists tailor their monitoring equipment so that the auditory alarms are more informative. However, the occurrence of tailoring by anaesthetists in the operating theatre was infrequent, even though the flexibility to tailor exists on many of the patient monitoring systems used in the study. We present an influence diagram to explain how alarm tailoring can increase situation awareness in the operating theatre but why factors inhibiting tailoring prevent widespread use. Extending the influence diagram, we discuss ways that more informative displays could achieve the results sought by anaesthetists when they tailor their alarm systems. In particular, we argue that we should improve our designs rather than simply provide more flexible tailoring systems, because users often find tailoring a complex task. We conclude that properly designed auditory displays may benefit anaesthetists in achieving greater patient situation awareness and that designers should consider carefully how factors promoting and inhibiting tailoring will affect the end-users' likelihood of conducting tailoring. © 2004 Elsevier B.V. All rights reserved.
Antagonistic effects of TGF-<i>β</i>1 and MSF on fibroblast migration and hyaluronic acid synthesis: Possible implications for dermal wound healing (1992)

The migration of adult skin fibroblasts into three-dimensional collagen gel matrices is differentially affected by cell density, with subconfluent cells displaying a significantly elevated level of migration compared to confluent ones. Fetal fibroblasts differ from adult cells in that they display an elevated level of migration at both subconfluent and confluent cell densities. We have previously reported that this difference in behaviour results from the secretion by fetal fibroblasts of a 'migration stimulating factor' (MSF) which is not made by their normal adult counterparts, and that MSF appears to act by stimulating the synthesis of hyaluronic acid (HA). Data presented in this communication indicate that (a) MSF specifically stimulates the synthesis of high molecular weight species of HA, (b) TGF-beta 1 inhibits the elevated migration of adult fibroblasts plated at subconfluent cell density, (c) under these conditions, TGF-beta 1 induces a parallel decrease in the synthesis of high molecular weight HA and increase in the synthesis of low molecular weight HA, (d) TGF-beta 1 is a potent antagonist of MSF, effectively blocking its stimulation of cell migration and synthesis of high molecular weight HA, and (e) the inhibition of fibroblast migration by TGF-beta 1 does not appear to be a chemotactic response dependent upon the existence of a concentration gradient of the cytokine. Our observations regarding the inhibitory effects of TGF-beta 1 on fibroblast migration into 3D collagen gels stand in marked contrast to various published reports indicating that this cytokine stimulates the migration of human skin fibroblasts through the pores of polycarbonate filters as used in modified Boyden chamber assays; this discrepancy underscores the importance of the substratum in modulating cellular response to cytokines. Our results are discussed in terms of the possible combined contribution of MSF and TGF-beta 1 to wound healing.
Basis of neurovirulence of avirulent rabies virus variant Av01 with stereotaxic brain inoculation in mice (1992)

Av01 is a variant of the challenge virus standard strain of fixed rabies virus that was selected with a neutralizing anti-glycoprotein monoclonal antibody, and has a single amino acid change in the glycoprotein. It is avirulent after both intracerebral and peripheral routes of inoculation in adult mice. In this study, Av01 was found to be neurovirulent with stereotaxic brain inoculation in either the striatum or cerebellum of adult mice. Mice that had been inoculated simultaneously with Av01 by the intracerebral and intrastriatal routes recovered. More infectious virus was present in the brains of mice inoculated intrastriatally than intracerebrally, and more neurons contained rabies virus antigen. However, the topographical distribution of infected neurons was similar with both routes. Serum neutralizing antibodies against rabies virus were produced later and in smaller quantities after intrastriatal inoculation. Av01 is probably neurovirulent after stereotaxic brain inoculation because this route produces both a direct site of viral entry into the central nervous system and a low level of immune stimulation.
Mutations in core nucleotide sequence of hepatitis B virus correlate with fulminant and severe hepatitis. (1993)

Infection with hepatitis B virus leads to a wide spectrum of liver injury, including self-limited acute hepatitis, fulminant hepatitis, and chronic hepatitis with progression to cirrhosis or acute exacerbation to liver failure, as well as an asymptomatic chronic carrier state. Several studies have suggested that the hepatitis B core antigen could be an immunological target of cytotoxic T lymphocytes. To investigate the reason why the extreme immunological attack occurred in fulminant hepatitis and severe exacerbation patients, the entire precore and core region of hepatitis B virus DNA was sequenced in 24 subjects (5 fulminant, 10 severe fatal exacerbation, and 9 self-limited acute hepatitis patients). No significant change in the nucleotide sequence and deduced amino acid residue was noted in the nine self-limited acute hepatitis patients. In contrast, clustering changes in a small segment of 16 amino acids (codon 84-99 from the start of the core gene) in all seven adr subtype infected fulminant and severe exacerbation patients was found. A different segment with clustering substitutions (codon 48-60) was also found in seven of eight adw subtype infected fulminant and severe exacerbation patients. Of the 15 patients, 2 lacked precore stop mutation which was previously reported to be associated with fulminant hepatitis. These data suggest that these core regions with mutations may play an important role in the pathogenesis of hepatitis B viral disease, and such mutations are related to severe liver damage.
Effects of Ischemia on Cerebral Arteriolar Dilation to Arterial Hypoxia in Piglets (1998)

Cerebrovascular responsiveness is selectively affected by anoxic stress. In addition, cerebral arteriolar dilation to hypoxia and adenosine is maintained after ischemia despite the expected impairment in K(ATP) function.