gebhart/patrepeval huggingface dataset:
Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein (2017)

Determination of proteins, especially low-abundance proteins with high sensitivity and specificity, is essential for characterizing proteomes and studying their biochemical functions. Herein, a novel Magnetic-Immuno-Loop-Mediated Isothermal Amplification (Im-LAMP) based on DNA-encapsulating liposomes (liposome-Im- LAMP), was developed for trace amounts of proteins. To the best of our knowledge, this is our first report about the magnetic Im-LAMP approach based on liposomes encapsulated template DNA as the detection reagent. The DNA template was released from liposomes and then initiated an Im-LAMP reaction, generating the fluorescence signal with high sensitivity and rapidity. This technique was applied for the determination of P-glycoprotein as a model protein. It was demonstrated that the technique exhibited a dynamic response to P-glycoprotein ranging from 1.6*10<sup>-2</sup> to 160 pg/ml with a greatly low detection limit of 5*10<sup>-3</sup> pg/ml (5 fg/ml) which is substantially better than conventional enzyme-linked immunosorbent assays (ELISA). This ultra sensitivity was attributed to the LAMP reaction initiated by the enormous DNA targets encapsulated in liposomes. This magnetic liposome-Im-LAMP as an alternative approach is attractive for applications in other low-abundance proteins detection in clinical diagnostics.
Tandem intramolecular Michael addition and 1,3-dipolar cycloaddition reactions of oximes; versatile new carbon–carbon bond forming methodology (1987)

Oximes undergo intramolecular Michael addition to proximate electronegative alkenes, generating cyclic nitrones which can be trapped inter- and intra-molecularly in 1,3-dipolar cycloaddition reactions taking place either separately or in tandem.
EXPERIMENTAL AUTOIMMUNE MYASTHENIA GRAVIS AND MYASTHENIA GRAVIS: BIOCHEMICAL AND IMMUNOCHEMICAL ASPECTS* (1976)

Stucture of acetylcholine receptor protein (AChR) purified from Electrophorus electricus (eel) by affinity chromatography is described. AChR is detected in extracts from human muscle, rat muscle, and rat thymus. Rats immunized with eel AChR develop humoral antibodies, a small fraction of which recognize AChR from rat muscle. Rats immunized with AChR exhibit myasthenia, but those immunized with denatured AChR do not. Immunoglobulin fraction of antisera to eel AChR can block the activity of AChR in electroplaques. Sera from patients with myasthenia gravis contain antibodies to AChR from human muscle detectabe at an average value 300-fold the background level in sera from nonmyasthenics. Relationship of thymoma and disease intensity to antibody titer is examined. The chronic phase of EAMG appears a good model of MG, since in both cases similar concentrations of 7-S immunoglobulin against determinants on muscle AChR other than the toxin binding site are found. Assay of anti-AChR antibody in sera from MG patients using AChR from rat muscle gives titers 10%-15% of those obtained using AChR from human muscle, and using AChR from eel gives negligible titers. The assay method described for assaying antibodies against AChR from human muscle is suggested as a diagnostic test for MG.
<i>Ex vivo</i> expansion of mature human neutrophils with normal functions from purified peripheral blood CD34<sup>+</sup> haematopoietic progenitor cells (2000)

Purified CD34+ haematopoietic progenitor cells were cultivated with stem cell factor, interleukin 3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte CSF (G-CSF) for 7 d, and thereafter non-adherent cells were divided into two groups. Cells in one group (group A) were further cultivated for 7 d with four cytokines, and cells in the other group (group B) were further cultivated for 7 d with G-CSF alone. On day 14, 220-fold and 130-fold increases in the numbers of non-adherent cells were achieved for groups A and B respectively. These cell preparations contained 65% granulocytes for group A and 95% granulocytes for group B. These cells gained the ability to respond effectively with chemotaxis, phagocytosis and superoxide (O2-) release. Cells in group B were appropriately primed by G-CSF, GM-CSF, tumour necrosis factor alpha and IL-1beta for enhanced release of O2 -. The responsiveness of these cells was identical to that of peripheral blood neutrophils, indicating that cells in group B may be in the resting state. In contrast, cells in group A were not primed by these cytokines for enhanced release of O2- and released a large amount of O2- spontaneously, indicating that cells in group A may be in the activated state. These findings indicate that mature neutrophils with normal functions were expanded ex vivo in group B and suggest that these cells could be used for possible autologous neutrophil transfusion to prevent bacterial infections during severe neutropenia after cytotoxic chemotherapy.
l-Octen-3-ol, an Effective Attractant for Tabanidae (Diptera) (1989)

Journal Article l-Octen-3-ol, an Effective Attractant for Tabanidae (Diptera) Get access Frank E. French, Frank E. French 1Department of Biology and Institute of Arthropodology and Parasitology, Georgia Southern College, Statesboro, Ga. 30460 Search for other works by this author on: Oxford Academic PubMed Google Scholar Daniel L. Kline Daniel L. Kline 2Insects Affecting Man and Animals Research Laboratory, USDA-ARS, Gainesville, Fla. 32604 Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Medical Entomology, Volume 26, Issue 5, 1 September 1989, Pages 459–461, https://doi.org/10.1093/jmedent/26.5.459 Published: 01 September 1989 Article history Received: 09 February 1988 Accepted: 10 January 1989 Published: 01 September 1989
Bridging the gap between climate models and impact studies: the <scp>FORESEE</scp> Database (2015)

Studies on climate change impacts are essential for identifying vulnerabilities and developing adaptation options. However, such studies depend crucially on the availability of reliable climate data. In this study, we introduce the climatological database called FORESEE (Open Database for Climate Change Related Impact Studies in Central Europe), which was developed to support the research of and adaptation to climate change in Central and Eastern Europe: the region where knowledge of possible climate change effects is inadequate. A questionnaire-based survey was used to specify database structure and content. FORESEE contains the seamless combination of gridded daily observation-based data (1951-2013) built on the E-OBS and CRU TS datasets, and a collection of climate projections (2014-2100). The future climate is represented by bias-corrected meteorological data from 10 regional climate models (RCMs), driven by the A1B emission scenario. These latter data were developed within the frame of the ENSEMBLES FP6 project. Although FORESEE only covers a limited area of Central and Eastern Europe, the methodology of database development, the applied bias correction techniques, and the data dissemination method, can serve as a blueprint for similar initiatives.
Design and Construction of the Leir Injection Septa (2006)

The Low Energy Ion Ring (LEIR) transforms long pulses from Linac 3 into high brilliance ion bunches for LHC by means of multi-turn injection, electron cooling and accumulation. The LEIR injection comprises a magnetic DC septum followed by an inclined electrostatic septum. The electrostatic septum has been newly designed and built. The magnetic septum is mainly recovered from the former LEAR machine, but required a new vacuum chamber. Dynamic vacua in the 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-12</sup> mbar range are required, which are hard to achieve due to the high desorption rate of ions lost on the surface. A new interlock and displacement control system has also been developed. The major technical challenges to meet the magnetic, electrical and vacuum requirements will be discussed.
Effects of Orexins on the Hypothalamic‐Pituitary‐Adrenal System (2000)

The effects of the recently identified neuropeptides orexin-A and orexin-B on the hypothalamic-pituitary-adrenal (HPA) system were investigated. An in vivo system was used to assess the central effects of both orexin-A and orexin-B. Different doses of the orexins (2.8-560 pmol) were administered intracerebroventricularly (i.c.v.) to adult male rats, and plasma corticosterone was used as an index of the degree of the activation of the HPA system. Both peptides exhibited a clear dose-response action, although orexin-B proved to be less effective than orexin-A. Pretreatment with the corticotropin-releasing hormone (CRH) antagonist alpha-helical CRH9-41 completely prevented the action of the orexins. Orexin-A, orexin-B or adrenocorticotropic hormone (ACTH) was further administered intraperitoneally (i.p.). While ACTH evoked a significant adrenal response, the orexins did not influence the basal secretion. Adrenal slices, oxygenized and perifused with Krebs' solution, were also treated with orexin-A, orexin-B or ACTH. Both orexins failed to modify the release of corticosterone, but ACTH induced a marked adrenal response. This study suggests that these appetite-regulating peptides might activate the HPA system at a central level but neither orexin-A nor orexin-B appears to modulate directly the adrenal corticosterone release.
Interconnection of Micropad Electrodes by Controlled “Extraneous” Deposition of Electroless NiB Film (2007)

A method of interconnecting micropad electrodes was developed by using the electroless deposition of for application to the flip-chip bonding technology. The phenomenon of "bridge" formation by the so-called "extraneous deposition" was utilized as a technique to perform selective deposition of on noncatalytic surfaces. The process of extraneous electroless deposition was controlled by optimizing deposition conditions so that the deposit grew anisotropically along the surface between the facing pads. Practical feasibility of this technique was demonstrated by forming an interconnection between pads separated by and measuring in both height and width. This method was applied successfully to interconnecting the pads forming an array with a pitch of .
Synthesis, Crystal Structure, Spectroscopic Properties and Potential Biological Activities of Salicylate‒Neocuproine Ternary Copper(II) Complexes (2015)

Mixed ligand copper(II) complexes containing derivatives of salicylic acid and heterocyclic ligands with nitrogen donor atoms have been the subject of various studies and reviews. In this paper, synthesis and characterization of the ternary copper(II) complexes of neocuproine (2,9-dimethyl-1,10-phenanthroline, Neo) and salicylate ligands (Sal) are reported. In addition, the crystal structures of ([Cu(H2O)(5-Cl-Sal)(Neo)] (1), [Cu(μ-Sal)(Neo)]2 (2), Cu2(μ-5-Cl-Sal)(5-Cl-HSal)2(Neo)2]·EtOH (3)) were determined. In order to compare structural and biological properties of the prepared complexes, spectroscopic and biological studies were performed. Results of X-ray diffraction show that prepared complexes form three types of crystal structures in a given system: monomeric, dimeric and dinuclear complex. The preliminary study on the DNA cleavage activity has shown that the complexes under study behave as the chemical nucleases in the presence of added hydrogen peroxide with slight differences in the activity (1 > 2 > 3). The complexes 1 and 2 exhibited nuclease activity itself indicating the interaction of complexes with the DNA. It has been proposed that the enhanced destructive effect of the complexes 1 and 2 on the DNA is a result of two possible mechanisms of action: (i) the conversion of closed circular DNA (form I) to the nicked DNA (form II) caused by the copper complex itself and (ii) damage of DNA by Reactive Oxygen Species (ROS)-products of the interaction of copper with hydrogen peroxide by means of Fenton reaction (hydroxyl radicals). Thus the biological activity of the prepared Cu(II) complexes containing derivatives of salicylic acid and phenanthroline molecules is substantiated by two independent mechanisms. While derivatives of salicylic acids in the coordination sphere of copper complexes are responsible for radical-scavenging activity (predominantly towards superoxide radical anion), the presence of chelating ligand 2,9-dimethyl-1,10-phenanthroline significantly enhances capability of Cu(II) complexes binding to DNA via intercalation.
Directly Printed Packaging-Paper-Based Chipless RFID Tag With Coplanar $LC$ Resonator (2013)

This letter presents the design, simulation, fabrication, and characterization of an <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LC</i> -resonator-based chipless RFID tag. The ID-generating circuit is designed based on a reconfigurable <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LC</i> resonance circuit. Phase position modulation (PPM) coding is used for the enhancement of the coding capacity. The tag has been realized on packaging paper using all printing technique. In fabrication, overprinting process has been investigated as an effective pathway for the improvement of the conductivities. The tag with 4.25-bit coding capacity has been examined using a sweeping frequency signal transmitted from a vector network analyzer, and experimental results confirm the feasibility of the proposed chipless tag. With further optimizations, the tag can be used in the item-level tracking and identification applications, especially for the management of paper tickets and banknotes.
Crystal Phase Transitions in the Shell of PbS/CdS Core/Shell Nanocrystals Influences Photoluminescence Intensity (2014)

We reveal the existence of two different crystalline phases, i.e., the metastable <i>rock salt</i> and the equilibrium <i>zinc blende</i> phase within the CdS-shell of PbS/CdS core/shell nanocrystals formed by cationic exchange. The chemical composition profile of the core/shell nanocrystals with different dimensions is determined by means of anomalous small-angle X-ray scattering with subnanometer resolution and is compared to X-ray diffraction analysis. We demonstrate that the photoluminescence emission of PbS nanocrystals can be drastically enhanced by the formation of a CdS shell. Especially, the ratio of the two crystalline phases in the shell significantly influences the photoluminescence enhancement. The highest emission was achieved for chemically pure CdS shells below 1 nm thickness with a dominant metastable <i>rock salt</i> phase fraction matching the crystal structure of the PbS core. The metastable phase fraction decreases with increasing shell thickness and increasing exchange times. The photoluminescence intensity depicts a constant decrease with decreasing metastable <i>rock salt</i> phase fraction but shows an abrupt drop for shells above 1.3 nm thickness. We relate this effect to two different transition mechanisms for changing from the metastable <i>rock salt</i> phase to the equilibrium <i>zinc blende</i> phase depending on the shell thickness.
An accurate model of subbreakdown due to band-to-band tunneling and its application (2003)

The authors describe a novel accurate model and numerical analysis of subbreakdown phenomena due to band-to-band tunneling in a thin-gate-oxide n-MOSFET. Subbreakdown I-V characteristics are calculated for various oxide thicknesses. The results agree with experimental results over a wide range of subbreakdown current from 10/sup -12/ A to 10/sup -6/ A. The numerical analysis based on this model has been utilized to suppress the subbreakdown current. It is concluded that the model can be utilized for the design of thin-gate-oxide devices.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Calibration free beam hardening correction using grangeat-based consistency measure (2016)

Due to polychromatic nature of the X-ray spectrum, beam hardening artifacts are introduced in cone beam computed tomography reconstructed images. To reduce these artifacts, projection images can be linearized with higher order polynomials. Polynomial coefficients are usually computed with calibration scans of homogeneous phantoms. This can be a challenging task when the objects with different material composition and geometry are scanned with different tube voltages. Here, we present a beam hardening correction method based on a consistency condition derived from Grangeat's formula for cone beam projections. First, an objective function is designed to quantify the inconsistency between two arbitrary projections. It is observed that inconsistency due to beam hardening is significantly high. Thus, by minimizing the objective function, polynomial parameters can be computed without prior knowledge about material composition, material thickness, x-ray spectrum or detector response. Since the consistency conditions based on Grangeat's formula can be applied to two arbitrary cone beam projections, the modeling of polynomials is very robust. Our preliminary results show that proposed correction significantly reduces beam hardening artifacts like cupping. For the first time, we show that projection linearization optimized by a consistency measure can be applied to real data from a flat panel detector scanner to reduce the beam hardening artifacts. The proposed method can also be extended for multi-material beam hardening correction by using two or more polynomials or by estimating an effective projection linearization for each view.
Quantum metrology with a single spin-$3/2$ defect in silicon carbide (2016)

We show that implementations for quantum sensing with exceptional sensitivity and spatial resolution can be made using spin-3/2 semiconductor defect states. We illustrate this using the silicon monovacancy deep center in hexagonal SiC based on our rigorous derivation of this defect's ground state and of its electronic and optical properties. For a single $\textrm{V}_{\textrm{Si}}^-$ defect, we obtain magnetic field sensitivities capable of detecting individual nuclear magnetic moments. We also show that its zero-field splitting has an exceptional strain and temperature sensitivity within the technologically desirable near-infrared window of biological systems. The concepts and sensing schemes developed here are applicable to other point defects with half spin multiplet ($S\geq 3/2$) configuration.
Thermochemistry of Sulfur Atom Transfer. Enthalpies of Reaction of Phosphines with Sulfur, Selenium, and Tellurium, and of Desulfurization of Triphenylarsenic Sulfide, Triphenylantimony Sulfide, and Benzyl Trisulfide (1998)

The enthalpies of reaction of neat PBu3 with solid sulfur (−27.1 ± 0.5 kcal/mol), selenium (−20.0 ± 0.6 kcal/mol), and tellurium (−4.9 ± 0.6 kcal/mol) have been measured by solution calorimetry. The enthalpies of reaction of a series of phosphines with sulfur in toluene solution have been measured as follows (values in kcal/mol): PCy3 = −30.9 ± 1.9, PBu3 = −28.9 ± 0.3, PMe3 = −27.1 ± 0.4, PMe2Ph = −26.0 ± 0.5, PMePh2 = −23.8 ± 0.3, PPh3 = −21.5 ± 0.3. These values correlate with literature data for enthalpies of protonation and indicate that P to S σ donation is probably the dominant factor in determining the R3PS bond strength, estimates for which range from 88 to 98 kcal/mol. The enthalpies of S atom transfer to PPh3 by SAsPPh3 and SSbPh3 in toluene solution are −17.7 ± 1.2 and − 21.5 ± 1.0 kcal/mol, respectively. The enthalpy of removal of the central S atom from BzSSSBz by PCy3, yielding BzSSBz and SPCy3, is − 29.0 ± 1.8 kcal/mol. These data are used to establish a range of enthalpies of S atom transfer in these compounds which spans 31 kcal/mol from SSbPPh3 to SPCy3.
People-Centred Public Health (2012)

<i>People-Centred Public Health</i> provides a comprehensive and accessible overview of policy, practice and research in how members of the public can be involved in delivering health improvement as volunteers or lay health workers, drawing on a major study of lay engagement in public health, and using case studies and real life examples.
Detection of serological biomarkers by proximity extension assay for detection of colorectal neoplasias in symptomatic individuals (2013)

Five plasma protein biomarkers were found to be potential CRC discriminators and three of these were additionally found to be discriminators of early-stage CRC. These explorative data in symptomatic individuals demonstrates the feasibility of the multiplex proximity extension assay for screening of potential serological protein biomarkers and warrants independent analyses in a larger sample cohort, including asymptomatic individuals, to further validate the performances of our CRC biomarker panel.
Technical Report - FINAL (2007)

Improve understanding of the earthquake hazard in the Las Vegas Valley and to assess the state of preparedness of the area's population and structures for the next big earthquake. 1. Enhance the seismic monitoring network in the Las Vegas Valley 2. Improve understanding of deep basin structure through active-source seismic refraction and reflection testing 3. Improve understanding of dynamic response of shallow sediments through seismic testing and correlations with lithology 4. Develop credible earthquake scenarios by laboratory and field studies, literature review and analyses 5. Refine ground motion expectations around the Las Vegas Valley through simulations 6. Assess current building standards in light of improved understanding of hazards 7. Perform risk assessment for structures and infrastructures, with emphasis on lifelines and critical structures 8. Encourage and facilitate broad and open technical interchange regarding earthquake safety in southern Nevada and efforts to inform citizens of earthquake hazards and mitigation opportunities
Cloning of the murine lymphocyte function-associated molecule-1 alpha-subunit and its expression in COS cells (1991)

The lymphocyte function-associated molecule 1 (LFA-1, CD11a/CD18) is an integrin that mediates adhesion of immune cells by interaction with two members of the Ig superfamily, ICAM-1 and ICAM-2. LFA-1 consists of an alpha subunit (Mr = 180,000) and a beta subunit (Mr = 95,000). We report here the isolation and expression of the murine alpha subunit cDNA (GenBank accession no. M60778). The deduced sequence comprises a 1061 amino acid extracellular domain, a 29 amino acid transmembrane region, and a 50 amino acid cytoplasmic domain. It has a 72% amino acid identity with its human counterpart and 34% identity with the murine Mac-1 alpha subunit. The murine LFA-1 alpha subunit could be expressed on the cell surface of a fibroblastoid cell line, COS, by cotransfection with either the human or murine beta subunit cDNA.
Neuroevolution of an automobile crash warning system (2005)

Many serious automobile accidents could be avoided if drivers were warned of impending crashes before they occurred. In this paper, a vehicle warning system is evolved to predict such crashes in the RARS driving simulator. The NeuroEvolution of Augmenting Topologies (NEAT) method is first used to evolve a neural network driver that can autonomously navigate a track without crashing. The network is subsequently impaired, resulting in a driver that occasionally makes mistakes and crashes. Using this impaired driver, a crash predictor is evolved that can predict how far in the future a crash is going to occur, information that can be used to generate an appropriate warning level. The main result is that NEAT can successfully evolve a warning system that takes into account the recent history of inputs and outputs, and therefore makes few errors. Experiments were also run to compare training offline from previously collected data with training online in the simulator. While both methods result in successful warning systems, offline training is both faster and more accurate. Thus, the results in this paper set the stage for developing crash predictors that are both accurate and able to adapt online, which may someday save lives in real vehicles.