gebhart/patrepeval huggingface dataset:
The in vitro HL-60 cell--Trypanosoma brucei rhodesiense culture system: a rapid in vitro drug screen. (1995)

The in vitro culture system is described in which Trypanosoma brucei rhodesiense (LOUTat.1) was grown with the human feed layer cell HL-60. The use of this system in determining the 50% growth Inhibitory Concentration (IC50) of unknown compounds for both the trypanosomes and the host cell was demonstrated. The data shows that several analogues of pentamidine have significantly reduced host cell toxicity but maintain or have increased typanocidal activity. The value of the trypanosome/HL-60 in vitro culture system as a rapid primary in vitro drug screen is discussed. Based upon the ability of this primary screen to predict potential drug efficacy, several analogues screened in vitro were then tested in vivo. The results of the in vivo tests confirmed the ability of the in vitro screen to predict drug efficacy, and also suggests that better analogues of pentamidine (less host toxicity and greater trypanocidal activity) can be obtained to treat human trypanosomiasis.
Formation of a single phosphodiester bond by RNA polymerase B from calf thymus is not inhibited by .alpha.-amanitin (1982)

The template-directed synthesis of a single phosphodiester bond by highly purified calf thymus RNA polymerase B is not inhibited by high concentrations of alpha-amanitin (10(-6) M). However, a subsequent internucleotide bond is not synthesized in the presence of alpha-amanitin. These results suggest that translocation of the nascent RNA and RNA polymerase B along the DNA template is the enzymatic process inhibited by alpha-amanitin. It is also shown that the formation of a single phosphodiester bond by RNA polymerase B results in a stable ternary transcription complex, i.e., between the enzyme, the DNA, and the nascent RNA. Under reaction conditions which normally favor the elongation of RNA, the transcriptional process is arrested at initiation by alpha-amanitin. Such ternary initiation complexes have been isolated by agarose gel electrophoresis.
Comprehensive Sequence-Flux Mapping of a Levoglucosan Utilization Pathway in <i>E. coli</i> (2015)

Synthetic metabolic pathways often suffer from low specific productivity, and new methods that quickly assess pathway functionality for many thousands of variants are urgently needed. Here we present an approach that enables the rapid and parallel determination of sequence effects on flux for complete gene-encoding sequences. We show that this method can be used to determine the effects of over 8000 single point mutants of a pyrolysis oil catabolic pathway implanted in Escherichia coli. Experimental sequence-function data sets predicted whether fitness-enhancing mutations to the enzyme levoglucosan kinase resulted from enhanced catalytic efficiency or enzyme stability. A structure of one design incorporating 38 mutations elucidated the structural basis of high fitness mutations. One design incorporating 15 beneficial mutations supported a 15-fold improvement in growth rate and greater than 24-fold improvement in enzyme activity relative to the starting pathway. This technique can be extended to improve a wide variety of designed pathways.
General iterative array (1972)

A general iterative array is described, which performs many useful switching functions as well as any combination of the fundamental arithmetic operations.
Time-Lapse Relation to Changes in the Respiratory Epithelium after Minimal Trauma (1964)

(1) A series of changes takes place in the respiratory epithelium in extirpated cow tracheas after a light swab has been passed over the surface. These take place over a period of 1 to 24 hours and are characterized by progressive loosening from one another of the columnar cells and cleavage from the underlying cells, with subsequent loss of cell form and complete exfoliation of the columnar layer. A new surface forms on the remaining cells, giving the appearance of a thin, flat epithelium. (2) During these changes, the epithelium becomes progressively more fragile and even, after just two hours, can usually be wiped away cleanly with a 5-gram swab. (3) The staining of the cells by methylene blue seems to be due to injury of the cell and is not dependent upon the presence or absence of overlying mucin. (4) No definite regeneration of epithelium could be determined in the extirpated trachea, even up to 72 hours, although there were suggestions of it.
Platelet metabolism and activation. (1985)

Stimulation and execution of platelet responses are intimately coupled to the cells' energy metabolism. The turnover of cytoplasmic ATP is higher than in most other cells; when it is lowered, platelet responses are powerfully inhibited. One-third of the total adenine nucleotides are present in the cytoplasm and a small fraction of these, 50% of the ADP, is bound to actin and not available to metabolism. These nucleotides are not synthesized de novo in platelets which readily form them from adenine, adenosine, and hypoxanthine. The remaining two-thirds are sequestered in the dense granules together with serotonin and divalent metal ions. NMR studies show that this ATP and ADP are stacked in large aggregates together with the divalent cations. Serotonin is incorporated into the aggregates by being positioned between, and in close proximity to, adjacent adenine rings. In the congenital platelet disorder, "Storage Pool Deficiency," the dense granule content is absent or markedly reduced, which may be caused by an impaired transport of ATP across the dense granule membrane. The content of the dense granules, the beta-thromboglobulin, albumin, fibrinogen, certain coagulation factors, fibronectin of the alpha-granules, and the acid hydrolases of the lysosomes are discharged by exocytosis, but the exact mechanisms are not known. This discharge, platelet secretion, is complete for the dense granule and alpha-granules contents, but is only 30% to 55% for the acid hydrolases. After pretreatment of platelets with certain amines, thrombin causes secretion of all beta-hexosaminidase, indicating that the beta-hexosaminidase usually retained during secretion is bound within the lysosomes at the acid pH but becomes dissociated by raising the pH with the amines. Agonist-induced platelet aggregation and secretion are accompanied by rapid changes in phosphoinositide metabolism which are usually monitored as the radioactivity in phospholipids of platelets preincubated with radioactive arachidonate, glycerol, inositol, or orthophosphate. The mechanisms for precursor incorporation are not well known and interpretations of published results are hampered by much uncertainty. Experimental support for the widely accepted coupling of receptor occupancy to the phosphodiesteratic hydrolysis of phosphatidylinositol or phosphatidylinositol-4,5-biphosphate is nonexistent. These two hydrolytic steps occur concomitantly and together with formation of diglyceride and phosphatidate; all four steps cannot be temporally resolved.(ABSTRACT TRUNCATED AT 400 WORDS)
A biotechnology of photosynthetic cells based on the use of solar energy (1980)

Conference Article| August 01 1980 A biotechnology of photosynthetic cells based on the use of solar energy CLAUDE GUDIN; CLAUDE GUDIN 1Laboratoire d'Heliosynthèse, British Petroleum, Lavera 13117, France Search for other works by this author on: This Site PubMed Google Scholar DANIEL CHAUMONT DANIEL CHAUMONT 1Laboratoire d'Heliosynthèse, British Petroleum, Lavera 13117, France Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1980) 8 (4): 481–482. https://doi.org/10.1042/bst0080481 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation CLAUDE GUDIN, DANIEL CHAUMONT; A biotechnology of photosynthetic cells based on the use of solar energy. Biochem Soc Trans 1 August 1980; 8 (4): 481–482. doi: https://doi.org/10.1042/bst0080481 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1980 Biochemical Society1980 Article PDF first page preview Close Modal You do not currently have access to this content.
[Inhibitory effect of free nitrous acid and cross inhibition in denitrification]. (2010)

Nitrite and nitrate have been generally recognized to have an inhibitory effect on metabolism of denitrifiers. The nitrite inhibition on nitrate reduction under various pH conditions and the cross effect of the both electron acceptors on each other were investigated through a series of batch tests by using a biological nutrient removal (BNR) sludge. The results showed that the nitrate reduction activity had a much stronger relationship with the free nitrous acid (FNA) than that of nitrite concentration, suggesting that FNA, rather than nitrite is likely the actual inhibitor on nitrate reduction. Sixty percent inhibition of nitrate reduction was observed at an FNA concentration of 0.01-0.025 mg x L(-1), while total inhibition occurred when the FNA concentration was greater than 0.2 mg x L(-1). Furthermore, nitrite reduction by the BNR sludge was also found to be inhibited by HNO2. The reduction rate decreased by approximately 80% when the FNA concentration was increased from 0.01 mg x L(-1) to 0.2 mg x L(-1). The inhibitory effect of nitrate on nitrite reduction was found to be insignificant, with the most recovery rates under different nitrate concentrations larger than 90%. However, the nitrate reduction rate was observed to recover only 3.04%-72.54%. The recovery rate from inhibition was independent of the duration of the inhibition and the feeding mode of inhibitor, but strongly dependent on the concentration of inhibitor the biomass was exposed to during the inhibition period.
Synthesis, X-ray diffraction structure, spectroscopic properties and antiproliferative activity of a novel ruthenium complex with constitutional similarity to cisplatin (2009)

The light-protected reaction of [(eta(6)-p-cymene)Ru(II)Cl(2)](2) with 1-(2-hydroxyethyl)piperazine in dry methanol, followed by addition of excess NH(4)PF(6), afforded the complex [(eta(6)-p-cymene)Ru(II)(NH(3))(2)Cl](PF(6)) () in 47% yield. Attempts to use the same protocol for the synthesis of [(eta(6)-p-cymene)Os(II)(NH(3))(2)Cl](PF(6)) led to the isolation of the binuclear triply methoxido-bridged arene-osmium compound [{(eta(6)-p-cymene)Os}(2)(mu-OCH(3))(3)](PF(6)) (). Both compounds were characterised by X-ray crystallography and (1)H NMR spectroscopy, and the ruthenium complex also by spectroscopic techniques (IR and UV-vis spectroscopies). The antiproliferative activity of complex in vitro was studied in A549 (non-small cell lung carcinoma), CH1 (ovarian carcinoma), and SW480 (colon carcinoma) cells and compared to that of [(eta(6)-p-cymene)Ru(II)(en)Cl](PF(6)) (). In contrast to the latter compound, is only modestly cytotoxic in all three cell lines (IC(50): 293-542 muM), probably due to the instability of the diammine ruthenium complex in aqueous solution.
Cell behavior on laser surface-modified polyethylene terephthalatein vitro (2001)

This work has been undertaken to study the cell behavior of L929 fibroblasts on the laser irradiated polyethylene terephthalate (PET) surface. To modify the surface properties of the PET, CO2 pulsed laser at the wavelength of 9.25 microm and KrF excimer laser at 248 nm with various number of pulses were used. Laser irradiation caused some changes in the chemical and physical properties of the laser-treated film surfaces, which were evaluated using different techniques. These changes may affect the cell adhesion and growth on the laser-treated PET. Therefore, cell attachment and spreading were investigated on the laser-treated PET in vitro. The data from in vitro assays showed the fibroblast cells were attached and proliferated extensively on the CO2 and KrF laser-treated films in comparison with the unmodified PET. The results obtained from the cell behavior studies revealed that surface morphology and wettability affected cell adhesion and spreading on the laser-treated PET.
Materials for thermoelectric energy conversion (1988)

The field of thermoelectric energy conversion is reviewed from both a theoretical and an experimental standpoint. The basic theory is introduced and the thermodynamic and solid state views are compared. An overview of the development of thermoelectric materials is presented with particular emphasis being placed on the most recent developments in high-temperature semiconductors. A number of possible device applications are discussed and the successful use and suitability of these devices for space power is manifest.
Efficient computation algorithm for calculating load contributions to line flows and losses (2006)

In a deregulated power system, it is usually required to determine the shares of each load and generation in line flows, to permit fair allocation of transmission costs between the interested parties. The paper presents a new method of determining the contributions of each load to line flows and losses. The method is based on power-flow topology and has the advantage of being the least computationally demanding of similar methods.
On-resonant phase-conjugate reflection and amplification at 106 μm in inverted CO_2 (1979)

We have obtained phase-conjugate reflection and amplification with an effective reflectivity exceeding unity by redirecting the output of a TEA CO(2)-laser oscillator into its own gain medium. The intense counterpropagating waves within the laser were coupled through the saturated medium to provide the nonlinearity in a process analogous to degenerate four-wave mixing.
Moral Reflections in Marketing (1998)

In this article, the authors introduce the concept of moral reflection and test several contextual variables thought to affect how much an individual engages in Business-Relevant Moral Reflection (BRMR). We find that the degree of organizational centralization is negatively related to BRMR and that organizational norms are positively related to BRMR. Additionally, we find that the greater importance an individual places on both pragmatic and moral values the more she or he thinks about business ethics.
Contribution of the<i>stg</i>Fimbrial Operon of<i>Salmonella enterica</i>Serovar Typhi during Interaction with Human Cells (2007)

Salmonella serovars contain a wide variety of putative fimbrial systems that may contribute to colonization of specific niches. Salmonella enterica serovar Typhi is the etiologic agent of typhoid fever and is a pathogen specific to humans. In a previous study, we identified a gene, STY3920 (stgC), encoding the predicted usher of the stg fimbrial operon, that was expressed by serovar Typhi during infection of human macrophages. The stg genes are located in the glmS-pstS intergenic region in serovar Typhi and certain Escherichia coli strains, but they are absent in other S. enterica serovars. We cloned the stg fimbrial operon into a nonfimbriate E. coli K-12 strain and into S. enterica serovar Typhimurium. We demonstrated that the stg fimbrial operon contributed to increased adherence to human epithelial cells. Transcriptional fusion assays with serovar Typhi suggested that stg is preferentially expressed in minimal medium. Deletion of stg reduced adherence of serovar Typhi to epithelial cells. However, deletion of stg increased uptake of serovar Typhi by human macrophages, and overexpression of stg in serovar Typhi and serovar Typhimurium strains reduced phagocytosis by human macrophages. These strains survived inside macrophages as well as the wild-type parent. Although the stgC gene contains a premature stop codon that disrupts the expected open reading frame encoding the usher and is therefore considered a pseudogene, our results show that the stg operon may encode a functional fimbria. Thus, this serovar Typhi-specific fimbrial operon contributes to interactions with host cells, and further characterization is important for understanding the role of the stg fimbrial cluster in typhoid fever pathogenesis.
A Novel Type of Antioxidant Isolated from Leaf Wax of<i>Eucalyptus</i>leaves (1981)

As a search for natural antioxidants from plant materials, strong antioxidative activity was observed in leaf waxes extracted from Eucalyptus species. A novel type of antioxidant was isolated from the leaf wax of Eucalyptus globulus and identified as n-tritriacontan-16,18-dione. Antioxidative activities were determined by different methods; a thiocyanate method, a thiobarbituric acid method, a total carbonyl value method and a weighing test. The anti-oxidant showed remarkable antioxidative activity in a water/alcohol system and was more effective than α-tocopherol and BHA; however, it has no antioxidative activity in an oil system.
The replication of DNA containing the simian virus 40 origin by the monopolymerase and dipolymerase systems. (1992)

The influence of DNA polymerase (pol) alpha and DNA primase on SV40 DNA replication was examined in both the monopolymerase and dipolymerase systems. The synthesis of oligoribonucleotides in the monopolymerase and dipolymerase systems, followed by pulse labeling with deoxynucleoside triphosphates, yielded short Okazaki fragments approximately 35 nucleotides in length that were chased into full-length Okazaki fragments with time. In the presence of activator 1 and proliferating cell nuclear antigen (PCNA), but no pol delta, these short fragments hardly increased in size with time. DNA fragments of similar size (approximately 35 nucleotides) were previously observed in SV40 replication reactions carried out with crude extracts of HeLa cells in the presence of antibodies directed against PCNA (Bullock, P. A., Seo, Y.S., and Hurwitz, J. (1991) Mol. Cell. Biol. 11, 2350-2361). Thus, the pol alpha-primase complex appears to act processively for only a short distance. At high levels of pol alpha and primase, both short and long DNA products were formed in both systems. In the presence of limiting amounts of pol alpha and excess primase, the monopolymerase system inefficiently yielded longer length Okazaki fragments than those formed with excess pol alpha and primase, whereas the dipolymerase system yielded both short and long DNA fragments. In the presence of limiting amounts of primase and excess pol alpha, long products were formed in both systems, and virtually no short products accumulated. Thus, the ratio between the polymerase and primer ends available controls the size of the nascent product DNA strands. We examined whether PCNA, the T4 phage-encoded gene product 45 (T4 gp45), and the Escherichia coli beta subunit of DNA polymerase III (dnaN gene product) supported SV40 DNA replication and the elongation of single-stranded DNA-binding protein-coated singly primed DNA in reactions catalyzed by pol delta, T4 DNA pol, and E. coli DNA pol III*, respectively. In the presence of T4 gp44/62 and T4 gp32 (but not human single-stranded DNA-binding protein isolated from HeLa cells), T4 DNA pol was weakly activated by PCNA and the beta subunit in lieu of T4 gp45 in the elongation of singly primed phi X174 DNA. However, the other systems were specific for their analogous auxiliary factors. This specificity indicates the importance of protein-protein interactions.
Changes of soluble CD26 and CD30 levels correlate with response to interferon plus ribavirin therapy in patients with chronic hepatitis C (2006)

Chronic hepatitis C patients have a weak Th1 response as reflected by lower soluble CD26 levels and the levels are even lower in non-sustained responders. In sharp contrast, downregulation of Th2 response with serial changes of soluble CD30 level is associated with successful treatment of HCV infection.
Incorporating error recovery into the imprecise computation model (2003)

We describe optimal algorithms for incorporating error recovery in the imprecise computation model. In this model each task comprises a mandatory and an optional part. The mandatory part must be completed within the task's deadline even in the presence of faults and a reward function is associated with the execution of each optional part. We address the problem of optimal scheduling in an imprecise computation environment so as to maximize total reward while simultaneously guaranteeing timely completion of recovery operations when faults occur. Furthermore, in order to prevent run-time overhead we enforce that no changes in the optimal schedule should be necessary as long as no error is detected in mandatory parts. Independent imprecise computation tasks as well as tasks with an end-to-end deadline and linear precedence constraints are considered. We present polynomial-time optimal algorithms for models with upper and lower bounds on execution times of the optional parts and for reward functions represented by general nondecreasing linear and concave functions.
Baseband signal compression in wireless base stations (2012)

To comply with the evolving wireless standards, base stations must provide greater data rates over the serial data link between base station processor and RF unit. This link is especially important in distributed antenna systems and cooperating base stations settings. This paper explores the compression of baseband signal samples prior to transfer over the above-mentioned link. We study lossy and lossless compression of baseband signals and analyze the cost and gain of each approach. Sample quantizing is proposed as a lossy compression scheme and it is shown to be effective by experiments. With QPSK modulation, sample quantizing achieves a compression ratio of 4:1 and 3.5:1 in downlink and uplink, respectively. The corresponding compression ratios are 2.3:1 and 2:1 for 16-QAM. In addition, lossless compression algorithms including arithmetic coding, Elias-gamma coding, and unused significant bit removal, and also a recently proposed baseband signal compression scheme are evaluated. The best compression ratio achieved for lossless compression is 1.5:1 in downlink. Our simulations and over-the-air experiments suggest that compression of baseband signal samples is a feasible and promising solution for increasing the effective bit rates of the link to/from remote RF units without requiring much complexity and cost to the base station.
PROBABILISTIC COMPOSITE ANALYSIS DESIGN (1996)

A methodology and attendant computer code have been developed and are described for probabilistic composite design via computational simulation of the uncertain behavior of composite structures. The uncertain behavior that influences composite structural design includes buckling loads, stress concentration factors, displacements, stress/strain etc., which are the consequences of the inherent uncertainties (scatter) in the primitive (independent random) variables (constituent, ply, laminate and structural) that describe the composite structures. The computer computational performed by the code is IPACS (Integrated Probabilistic Assessment of Composite Structures). IPACS application to structural analysis is illustrated by its use to evaluate the uncertainties in the displacements and stresses of a typical wing section and the stresses of a composite bolted joint; while its application to probabilistic design is illustrated by its use to assess the thin composite stiffened shell.