gebhart/patrepeval huggingface dataset:
[Separation of immortalized mesenchymal stem cell like stromal cells of mouse embryonic aorta-gonad-mesonephros region and their biological characteristics]. (2008)

To investigate the effects of microenvironment of aorta-gonad-mesonephros (AGM) on embryonic hematopoiesis, mesenchymal stem cell like stromal cells (MSC like stromal cells) derived from dorsal aorta (DA) in AGM region were separated and identified by their growth characteristics, related molecules expression and mesenchymal lineage potentials. Stromal cells from DA region in mouse embryos (E11.5) were isolated and cultured in vitro. After transfected by pSV3neo-SV40, the clones with G418 resistance were selected, and their growth characteristics were studied. The related molecules were analyzed by flow cytometry, and each clone was induced to differentiate into adipocytes, osteocytes, and chondrocytes. The results showed that most clones (20 clones) selected in the mouse DA region held the morphology of fibroblastoid cells. mDAF3 and mDAF18 could be grown in culture for more than 50 passages with G418 resistance, both have the potential to differentiate into adipocytes, osteocytes, and chondrocytes. At the logarithmic growth period, the cell population doubling time is about 24 hours. Surface markers, such as CD29, CD44, CD105 and Sca-1 were positively detected, while low levels of CD34, CD45, and CD31 were detected. It is concluded that immortalized mDAF3 and mDAF18 have the specific phenotype and differential potency of MSC, which suggests that MSC maybe exist in mouse embryonic DA region, where the MSC like stromal cells can be used as a cell model for further research on the modulation activity of DA microenvironment for embryonic hematopoiesis.
원저 : 한국인 선IgA물집피부병의 임상연구 (2015)

Background: Linear IgA bullous dermatosis (LABD) is an autoimmune, chronic bullous disease characterized by sub-epithelial bullae with linear IgA deposits along the basement membrane. LABD primarily affects young children and adults. There has been no study on LABD in Korea to date. Objective: The purpose of this study was to evaluate the clinical features, laboratory examinations, treatments, and outcomes of Korean LABD patients. Patient characteristics including age at disease onset, gender, medical associations, medications, immunofluorescence findings, disease duration, treatment, and outcome were analyzed. Methods: A retrospective analysis was conducted on 16 LABD patients diagnosed at Gangnam Severance Hospital between 1999 and 2014. Results: A total of 16 LABD patients were included in the study, 5 children and 11 adults. The mean ages at disease onset in children and adults were 3.2 and 41 years, respectively. Eighty percent of children with LABD showed complete remission. In adults, partial remission was achieved in 36.4%, and complete remission in 54.5% of patients. Two patients were diagnosed with drug-induced LABD, and 2 with ulcerative colitis-associated LABD. Conclusion: Our report differed from previous reports in that all 5 children with LABD were male, and the incidence in adults was higher in females than males. Most patients responded well to dapsone and oral prednisolone. Since LABD is rare and can be misdiagnosed as impetigo or bullous pemphigoid, diagnosis by immunofluorescence microscopy is necessary for proper treatment to attain disease remission. (Korean J Dermatol 2015;53(7):515∼522)
Surface halides of silica. 2. Bromide and iodide (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSurface halides of silica. 2. Bromide and iodideM. P. McDanielCite this: J. Phys. Chem. 1981, 85, 5, 537–541Publication Date (Print):March 1, 1981Publication History Published online1 May 2002Published inissue 1 March 1981https://pubs.acs.org/doi/10.1021/j150605a016https://doi.org/10.1021/j150605a016research-articleACS PublicationsRequest reuse permissionsArticle Views114Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
[Changes of body weight and galanin in epileptic children treated with topiramate]. (2007)

Topiramate treatment may lower the body weight and reduce appetite in part of children with epilepsy which may be mediated by the reduced plasma galanin level.
Obstructive Sleep Apnea in Pregnancy and Fetal Outcome (2008)

It is generally agreed that pregnant women snore more than those who are not pregnant. Snoring in pregnancy has been associated with hypertension and is a risk factor for both preeclampsia and intrauterine growth restriction. Obstructive sleep apnea (OSA) may also be a cause of fetal compromise, since other disorders associated with maternal hypoxia can lead to adverse fetal outcomes. In this prospective observational study, both polysomnography (PSG) and the nonstress test (NST) were performed in pregnant women whose questionnaire responses suggested symptoms of OSA. Participating in the study were 35 women in whom the Berlin Scale questionnaire demonstrated symptoms of OSA. OSA was diagnosed by PSG in 4 of these women (11.4%). Two women had mild and 2 had moderate OSA. The women with OSA were older than the others and had higher body mass indices and larger neck circumferences. Two of the 4 women with OSA had gestational diabetes and one had cardiovascular disease. Three of the 31 women who did not have OSA had chronic hypertension, and 2 each had gestational diabetes, cardiovascular disease, and pregnancy-induced hypertension (preeclampsia). In 3 of the 4 cases of OSA, fetal heart decelerations accompanied maternal blood desaturation. The newborn infants had lower Apgar scores and birth weights than infants whose mothers did not have OSA. Three of the 4 infants whose mothers had OSA were admitted to a newborn healthcare unit. The investigators believe that, because early diagnosis and treatment of OSA may lessen adverse outcomes, all pregnant women should be checked for symptoms of OSA and those who are suspected should be offered PSG.
Measurement of modes in an overmoded circular waveguide (2002)

A magnetic and electric field probe is used to measure fields in a circular waveguide to determine the strength of three propagating modes. The mode strengths travelling in both the forward and reverse directions are determined at the source and at the load. Substantial mode conversion is found to exist and the losses in the aluminium waveguide are found to be substantially larger than predicted by theory.
Pollen-stigma interactions in <i>Brassica</i>: IV. Structural reorganization in the pollen grains during hydration (1986)

With the aid of osmium tetroxide vapour, dry pollen and pollen at various stages of hydration has been fixed anhydrously for examination with the transmission electron microscope (TEM). In addition to establishing features characteristic of grains at different states of hydration, this technique has enabled the detection of a superficial layer investing both the exine and the pollen coating. This layer, some 10 nm in depth, binds both lanthanum and Alcian Blue and is shown to be the first component of the pollen grain to make contact with the stigmatic pellicle. The use of vapour fixation has also rendered it possible to chart the passage of water into the pollen grains with great accuracy, for each level of hydration displays a strikingly different cytoplasmic organization. For example, dry pollen is characterized by the presence of unusual structures at the protoplast surface and large numbers of spherical fibrillar bodies, whilst the protoplast of hydrating pollen is conspicuously stratified and contains a peripheral layer of membranous cisternae, subjacent to which is a fibrillar matrix derived from the spherical bodies found in the dry grains. Vapour-fixed, fully hydrated pollen resembles conventionally fixed grains. The pollen coating appears electron-translucent after anhydrous fixation and contains discrete, slightly rounded bodies some 50 nm in diameter. The uptake of water by grains on the stigma is accompanied by conspicuous structural changes in this layer for, after a short period in contact with the papillar surface, the spherical bodies rapidly disappear and the coat becomes electron-opaque. Close examination of this 'converted' coating reveals the presence of membranous vesicles and other structural components.
Structure of the Plasmodium falciparum Circumsporozoite Protein, a Leading Malaria Vaccine Candidate (2009)

The Plasmodium falciparum circumsporozoite protein (CSP) is critical for sporozoite function and invasion of hepatocytes. Given its critical nature, a phase III human CSP malaria vaccine trial is ongoing. The CSP is composed of three regions as follows: an N terminus that binds heparin sulfate proteoglycans, a four amino acid repeat region (NANP), and a C terminus that contains a thrombospondin-like type I repeat (TSR) domain. Despite the importance of CSP, little is known about its structure. Therefore, recombinant forms of CSP were produced by expression in both Escherichia coli (Ec) and then refolded (EcCSP) or in the methylotrophic yeast Pichia pastoris (PpCSP) for structural analyses. To analyze the TSR domain of recombinant CSP, conformation-dependent monoclonal antibodies that recognized unfixed P. falciparum sporozoites and inhibited sporozoite invasion of HepG2 cells in vitro were identified. These monoclonal antibodies recognized all recombinant CSPs, indicating the recombinant CSPs contain a properly folded TSR domain structure. Characterization of both EcCSP and PpCSP by dynamic light scattering and velocity sedimentation demonstrated that both forms of CSP appeared as highly extended proteins (R(h) 4.2 and 4.58 nm, respectively). Furthermore, high resolution atomic force microscopy revealed flexible, rod-like structures with a ribbon-like appearance. Using this information, we modeled the NANP repeat and TSR domain of CSP. Consistent with the biochemical and biophysical results, the repeat region formed a rod-like structure about 21-25 nm in length and 1.5 nm in width. Thus native CSP appears as a glycosylphosphatidylinositol-anchored, flexible rod-like protein on the sporozoite surface.
Human P2Y<sub>6</sub> Receptor:  Molecular Modeling Leads to the Rational Design of a Novel Agonist Based on a Unique Conformational Preference (2005)

Combining molecular dynamics (MD) in a hydrated phospholipid (DOPC) bilayer, a Monte Carlo search, and synthesis of locked nucleotide analogues, we discovered that the Southern conformation of the ribose is preferred for ligand recognition by the P2Y(6) receptor. 2'-Deoxy-(S)-methanocarbaUDP was found to be a full agonist of the receptor and displayed a 10-fold higher potency than that for the corresponding flexible 2'-deoxyUDP. MD results also suggested a conformational change of the second extracellular loop consequent to agonist binding.
Phosphoinositide second messenger system is enriched in striosomes: immunohistochemical demonstration of inositol 1,4,5-trisphosphate receptors and phospholipase C beta and gamma in primate basal ganglia (1993)

The neurochemical organization of the basal ganglia has been studied extensively with respect to neurotransmitters, neuropeptides, and their receptors. The chemoarchitecture of the striatum has been found particularly striking, because it distinguishes many substances by their relative distributions within the striosome and matrix compartments of the striatum. Very little is yet known about the differential distribution of second messenger systems in the basal ganglia, however, and no information is available about whether the distribution of second messenger systems is related to the prominent neurochemical compartmentalization of the striatum. We have examined the distribution of the phosphoinositide second messenger system in the primate basal ganglia and substantia nigra, as detected with polyclonal antisera against the inositol 1,4,5-trisphosphate receptor (IP3R), and monoclonal antisera against phospholipase C beta (PLC beta) and phospholipase C gamma (PLC gamma). In the striatum, immunostaining for each of the three proteins was present predominantly in medium-sized neuronal perikarya and in the neuropil. Circumscribed zones of enhanced IP3R, PLC beta, and PLC gamma immunoreactivity appeared in a background of generally weaker staining, and these zones corresponded to striosomes as identified by calbinidin D28k and substance P immunostaining in adjacent sections. Thus, the richest representation of the phosphoinositide system in the primate striatum appears to be in striosomes. In the substantia nigra pars compacta, neurons and neuropil were immunopositive, but in the substantia nigra pars reticulata and in each segment of the globus pallidus, immunostaining was mainly confined to the neuropil. Perikaryal PCL gamma immunoreactivity in the absence of detectable PLC beta or IP3R immunolabeling was found in the magnocellular neurons embedded in the medullary layer between the putamen and the globus pallidus. These observations demonstrate that the phosphoinositide second messenger system is selectively enhanced in neuronal subsystems of the basal ganglia, including striosomes, and suggest that signaling by phosphoinositide pathways elicits discrete effects on input-output processing by the basal ganglia.
Surface mediated synthesis of 2D covalent organic frameworks: 1,3,5-tris(4-bromophenyl)benzene on graphite(001), Cu(111), and Ag(110) (2009)

The on surface synthesis of a two-dimensional (2D) covalent organic framework from a halogenated aromatic monomer under ultra-high vacuum conditions is shown to be dependent on the choice of substrate.
Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure. (1990)

We have studied the expression of fibulin in cultured fibroblasts and determined its primary structure by cDNA cloning. Our results show that fibulin is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix when expressed by cultured cells or added exogenously to cell monolayers. In addition, we find that fibulin is present in plasma at a level of 33 +/- 3 micrograms/ml. Sequencing of multiple fibulin cDNAs indicates that a process of alternative splicing results in the expression of three fibulin transcripts. The transcripts encode overlapping polypeptides differing only in carboxy-terminal segments. Common to the three predicted forms of fibulin is a unique 537-amino acid-long cysteine-rich polypeptide and a 29-residue signal peptide. The amino-terminal portion of fibulin contains a repeated element with potential disulfide loop structure resembling that of the complement component anaphylatoxins C3a, C4a, and C5a as well as proteins of the albumin gene family. The bulk of the remaining portion of the molecule is a series of nine EGF-like repeats.
Correlation of moxalactam (LY127935) susceptibility tests by disk diffusion and agar plate dilution methods (1981)

Susceptibility of pathogenic aerobic bacteria to moxalactam (LY127935) was compared by two methods, diffusion from 30-microgram disks and agar plate dilution. The two methods gave a satisfactory degree of correlation when compared by linear regression, but the slope of the linear regression was significantly steeper for gram-negative bacilli (Enterobacteriaceae and Pseudomonas aeruginosa) than for coccal organisms (streptococci and staphylococci). The largest zone diameters by disk diffusion were found with Citrobacter, Proteus, Escherichia coli, Klebsiella, and Enterobacter strains. The error rate-bounded method of Metzler and DeHaan (J. Infect. Dis. 130:588-594, 1974) gave useful breakpoints of disk zone sizes for moxalactam resistance and susceptibility.
Mechanical response to hyperinflation of the two abdominal muscle layers (1989)

Abdominal muscle length changes and activity were directly examined in vivo with the use of the techniques of sonomicrometry and electromyography, respectively, in nine supine anesthetized dogs. Expiratory threshold loading was utilized to stimulate recruitment of the abdominal muscles, and lung inflations produced the passive relationships. The internal layer, consisting of the internal oblique and transversus abdominis, shortened more in expiration than the external layer, consisting of the external oblique and rectus abdominis. The internal oblique shortened to approximately 83% of its length at functional residual capacity vs. 98% for the external oblique (P less than 0.05). The results obtained during passive lung inflation indicate these internal muscles are also more influenced by changes in lung volume. The internal oblique lengthened to 115% of its length at functional residual capacity vs. 103% for external oblique at total lung capacity (P less than 0.05). The results suggest that anatomic division of the abdominal muscles into external and internal layers corresponds to functional differences in terms of both passive lengthening and active shortening during ventilation and that these differences imply variable functions of the two layers.
Identification of Wells with High CO2-Leakage Potential in Mature Oil Fields Developed for CO2-Enhanced Oil Recovery (2008)

Abstract Previous work, presented in SPE 106817, "Evaluation of the Potential for Gas and CO2 Leakage along Wellbores", described a method to predict the potential for wellbore leakage which primarily occurs in the shallow areas of a wellbore. The work presented here focuses on the potential for leakage to occur from the deep regions of a wellbore, particularly from viable oil reservoirs. The potential for wellbore leakage where CO2 emhanced oil recovery (EOR) or sequestration is or may be conducted is specifically investigated. The Alberta Energy Resources Conservation Board collects and maintains data regarding cement types used in primary cementing of casing strings, stimulation information and abandonment data. These data were used to determine the potential for deep wellbore leakage in the presence of CO2. This deep wellbore leakage potential is then coupled with shallow wellbore leakage potential to predict which wells may leak to other reservoirs, potable groundwater aquifers or to the atmosphere in a CO2 sequestration or EOR project. Cements with additives such as bentonite have been shown to be particularly susceptible to CO2 attack. Wells were screened for cement blends placed in the deeper sections of the wellbore during primary cementing. This information is useful in predicting if a wellbore will remain leak free if CO2 is placed in the reservoir and the wellbore is contacted. The stimulation method was evaluated to determine if injected CO2 may break through to existing wellbores prior to full reservoir sweep, thus decreasing the time that the wellbore could remain leak free. Stimulations such as hydraulic fracture, perforating and acidizing with pressure were deemed to increase the likelihood that wellbores would leak due to cement sheath cracking coupled with CO2 attack of cement and casing. The abandonment method was also evaluated. In Alberta the primary form of zonal abandonment utilizes a mechanical bridge plug capped with cement. The bridge plug material is expected to fail in the presence of CO2 due to CO2 attack on elastomers and cast iron. Two large field evaluations are presented as case studies.
Knitting Technology (2001)

Knitting & Crochet, Needlework, Organization, Storage & Trans Davis's Comprehensive Handbook of Laboratory and Diagnostic Tests With Health Informatics: Practical Guide for Healthcare and Information Technology Biomaterials Science, Third Edition: An Introduction to Materials in Medicine. A Practical Guide to SysML, Third Edition, fully updated for SysML version 1.4, provides a comprehensive and practical guide for modeling systems with SysML. With their unique perspective INCOSE Systems Engineering Handbook: A Guide for System Life Cycle Processes and Activities. INCOSE Knitting · Goodreads Knitting is a method by which yarn is manipulated to create a textile or fabric. A third method, called combination knitting, goes through the front of a knit stitch and the back of a purl stitch. The Columbia Electronic Encyclopedia, Sixth Edition. Knitting Technology: a Comprehensive Handbook and Practical Guide.
High‐<i>T</i><sub>g</sub> Carbazole Derivatives as Blue‐Emitting Hole‐Transporting Materials for Electroluminescent Devices (2003)

Abstract A series of dicarbazolyl derivatives bridged by various aromatic spacers and decorated with peripheral diarylamines were synthesized using Ullmann and Pd‐catalyzed C–N coupling procedures. These derivatives emit blue light in solution. In general, they possess high glass‐transition temperatures ( T g > 125 °C) which vary with the bridging segment and methyl substitution on the peripheral amine. Double‐layer organic light‐emitting devices were successfully fabricated using these molecules as hole‐transporting and emitting materials. Devices of the configuration ITO/HTL/TPBI/Mg:Ag (ITO: indium tin oxide; HTL: hole‐transporting layer; TPBI: 1,3,5‐tris( N ‐phenylbenzimidazol‐2‐yl)benzene) display blue emission from the HTL layer. The EL spectra of these devices appear slightly distorted due to the exciplex formation at the interfaces. However, for the devices of the configuration ITO/HTL/Alq 3 /Mg:Ag (Alq 3 = tris(8‐hydroxyquinoline)aluminum) a bright green light from the Alq 3 layer was observed. This clearly demonstrates the facile hole‐transporting property of the materials described here.
<i>Cell Number Regulator1</i>Affects Plant and Organ Size in Maize: Implications for Crop Yield Enhancement and Heterosis   (2010)

Genes involved in cell number regulation may affect plant growth and organ size and, ultimately, crop yield. The tomato (genus Solanum) fruit weight gene fw2.2, for instance, governs a quantitative trait locus that accounts for 30% of fruit size variation, with increased fruit size chiefly due to increased carpel ovary cell number. To expand investigation of how related genes may impact other crop plant or organ sizes, we identified the maize (Zea mays) gene family of putative fw2.2 orthologs, naming them Cell Number Regulator (CNR) genes. This family represents an ancient eukaryotic family of Cys-rich proteins containing the PLAC8 or DUF614 conserved motif. We focused on native expression and transgene analysis of the two maize members closest to Le-fw2.2, namely, CNR1 and CNR2. We show that CNR1 reduced overall plant size when ectopically overexpressed and that plant and organ size increased when its expression was cosuppressed or silenced. Leaf epidermal cell counts showed that the increased or decreased transgenic plant and organ size was due to changes in cell number, not cell size. CNR2 expression was found to be negatively correlated with tissue growth activity and hybrid seedling vigor. The effects of CNR1 on plant size and cell number are reminiscent of heterosis, which also increases plant size primarily through increased cell number. Regardless of whether CNRs and other cell number-influencing genes directly contribute to, or merely mimic, heterosis, they may aid generation of more vigorous and productive crop plants.