gebhart/patrepeval huggingface dataset:
Novel and ultrasensitive noncompetitive enzyme immunoassay (hetero‐two‐site complex transfer enzyme immunoassay) for α‐human atrial natriuretic peptide (1992)

A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyrene ball. After washing, biotinylated alpha-hANP was eluted from the polystyrene ball with HCI and was reacted with 2,4-dinitrophenyl-fluorescein-bovine serum albumin-disulfide-rabbit anti-alpha-hANP [6-28] IgG conjugate. The complex formed was trapped onto (anti-2,4-dinitrophenyl group) IgG-coated polystyrene balls and, after washing, reacted with avidin-beta-D-galactosidase conjugate. The polystyrene balls were washed, and the complex of the three components was eluted with epsilon N-2, 4-dinitrophenyl-L-lysine and transferred to anti-fluorescein IgG-coated polystyrene balls. After washing, the complex was released from the polystyrene balls by reduction with 2-mercaptoethylamine and transferred to (anti-rabbit IgG) IgG-coated polystyrene balls. beta-D-Galactosidase activity bound to the last polystyrene balls was assayed by fluorometry. The detection limit of alpha-hANP [1-28] was 3 fg (1 amol)/tube. Interference by plasma proteins was eliminated by separation of peptides from proteins using a molecular sieve. The assay range of plasma alpha-hANP [1-28] was 0.04-120 ng/L, and plasma levels of hANP in healthy subjects (11-56 ng/L) were measured without concentration.
Measurement of Pressure and Density Inside a Single Sonoluminescing Bubble (2006)

The average pressure inside a sonoluminescing bubble in sulfuric acid has been determined by two independent techniques: (1) plasma diagnostics applied to Ar atom emission lines, and (2) light scattering measurements of bubble radius vs time. For dimly luminescing bubbles, both methods yield intracavity pressures approximately 1500 bar. Upon stronger acoustic driving of the bubble, the sonoluminescence intensity increases 10,000-fold, spectral lines are no longer resolved, and radius vs time measurements yield internal pressures > 3700 bar. Implications for a hot inner core are discussed.
Hypoxia in DU-145 Prostate Cancer Xenografts after Estramustine Phosphate and Radiotherapy (2010)

Purpose: Practically all solid malignant tumours have central hypoxia, which makes them less vulnerable to most forms of both radiotherapy and chemotherapy. Estramustine phosphate (EMP) is known to sensitise cancer cells and tumours to irradiation and to increase blood flow in malignant tumours. This study was designed to assess the effect of EMP and radiotherapy on the oxygenation status of the tumours. Methods and Materials: Nude mice with DU 145 human prostate cancer cell tumours were divided into four groups: The group ER was treated with EMP 0.2 mg/d and external irradiation 3 x 6 Gy; group E received EMP, group R irradiation only; and group O no treatment. The degree of hypoxia in the tumours before and after treatment was measured using 18F-labelled fluoromisonidazole ([18F]FMISO) as a marker. The testis served as a control organ. Histological samples were studied for necrosis and proliferation (DAPI-stain for mitoses and Ki-67). Results: The tumours showed more [18F]FMISO uptake, indicating more hypoxia, than the testes in all four groups. EMP did not improve tumour oxygenation but enhanced the ability of radiotherapy to cause tumour necrosis. Conclusions: The angiogenic effect of EMP, if present, is not crucial in the mechanism of radiosensitation. Keywords: Estramustine, radiotherapy, fluoromisonidazole, hypoxia, DU-145
Diffusion Bonding of Monoclinic Yb:KY(WO4)2/KY(WO4)2 and its Continuous-Wave Laser Operation (2008)

Diffusion bonding of the strongly anisotropic Yb:KY(WO4)2 and KY(WO4)2 crystals was successfully demonstrated. Efficient continuous-wave laser operation with slope efficiencies as high as 70% and 811 mW output power was obtained.
An energy-aware multipath-TCP-based content delivery scheme in heterogeneous wireless networks (2013)

IETF-proposed Multipath TCP (MPTCP) extends the standard TCP and allows data streams to be delivered across multiple simultaneous connections and consequently paths. The multipath capability of MPTCP provides increased bandwidth for applications in comparison with the classic single-path TCP, which makes it highly attractive for the current consumer mobile devices that support more than one radio interfaces (e.g. 3G, WiFi, Bluetooth, etc.). However, MPTCP does not consider energy consumption aspects which are highly important for these devices. This paper proposes eMTCP, a novel energy-aware MPTCP-based content delivery scheme which balances support for increased throughput with energy consumption awareness. eMTCP is located at upper transport layer in mobile devices and requires no additional modifications of the MPTCP-enabled server. eMTCP increases the energy efficiency of mobile devices by offloading traffic from the more energy-consuming interfaces to others. Simulation-based experiments employing an eMTCP model which sends data streams via the 3GPP Long Term Evolution (LTE) and IEEE 802.11 (WiFi) interfaces show an increase of up to 14% in energy efficiency when using eMTCP in comparison with MPTCP and of up to 66% in terms of quality in comparison with single-path TCP.
Generating urban road intersection models from low-frequency GPS trajectory data (2018)

Detailed real-time road data are an important prerequisite for navigation and intelligent transportation systems. As accident-prone areas, road intersections play a critical role in route guidance and traffic management. Ubiquitous trajectory data have led to a recent surge in road map reconstruction. However, it is still challenging to automatically generate detailed structural models for road intersections, especially from low-frequency trajectory data. We propose a novel three-step approach to extract the structural and semantic information of road intersections from low-frequency trajectories. The spatial coverage of road intersections is first detected based on hotspot analysis and triangulation-based point clustering. Next, an improved hierarchical trajectory clustering algorithm is designed to adaptively extract the turning modes and traffic rules of road intersections. Finally, structural models are generated via K-segment fitting and common subsequence merging. Experimental results demonstrate that the proposed method can efficiently handle low-frequency, unstable trajectory data and accurately extract the structural and semantic features of road intersections. Therefore, the proposed method provides a promising solution for enriching and updating routable road data.
Nursing the clinic and managing emotions in a fertility unit: Findings from an ethnographic study (2001)

This paper presents the findings from an ethnographic study of a fertility unit. Data were collected using participant observation and semi-structured interviews over a period of 2 years. Fifteen patients and 23 members of staff were interviewed. Data analysis was completed using a modified thematic analysis. The findings indicated that the emotions evoked by infertility and medical treatments were powerful and frightening. Patients managed their emotions privately although they were conscious of an emotional awareness by nurses, which they described as 'caring'. Nurses were associated strongly with caring and their role was primarily to manage emotions; however, to do this, nurses used non-caring (emotional distance) rather than caring. Nurses were responsible for managing emotions in the public spaces of the clinics and moved between emotional distance and awareness according to the needs of the clinic. In this article, it is argued that non-caring was a defence against anxiety about coping with painful feelings and that the nursing role was to 'nurse the clinic and the doctor' rather than the patient.
An absolute requirement for Cubitus interruptus in Hedgehog signaling (2001)

Hedgehog (Hh) proteins play diverse organizing roles in animal development by regulating gene expression in responding cells. Several components of the Hh signal transduction pathway have been identified, yet their precise role in mediating the various outputs of the pathway is still poorly understood. The Gli homolog Cubitus interruptus (Ci) is involved in controlling the transcription of Drosophila Hh target genes and thus represents the most downstream component known in this pathway. We address the question of whether the Hh pathway is distally branched or, in other words, whether the regulation of Ci activity is the sole output of Hh signaling. Putative Ci-independent branches of Hh signaling are explored by analyzing the behavior of cells that lack Ci but have undergone maximal activation of the Hh transduction pathway due to the removal of Patched (Ptc). The analysis of target gene expression and morphogenetic read-outs of Hh in embryonic, larval and adult stages indicates that Ci is absolutely required for all examined aspects of Hh outputs. We interpret this as evidence against the existence of Ci-independent branches in the Hh signal transduction pathway and propose that most cases of apparent Ci/Gli-independent Hh output can be attributed to the derepression of target gene expression in the absence of Ci/Gli repressor function.
GENE THERAPY ADVANCE Anti-HIV antibodies work inside cells (1993)

RETURN TO ISSUEPREVNewsNEXTGENE THERAPY ADVANCE Anti-HIV antibodies work inside cellsRON DAGANICite this: Chem. Eng. News 1993, 71, 33, 3–4Publication Date (Print):August 16, 1993Publication History Published online11 November 2010Published inissue 16 August 1993https://pubs.acs.org/doi/10.1021/cen-v071n033.p003https://doi.org/10.1021/cen-v071n033.p003newsACS PublicationsCopyright © 1993 AMERICAN CHEMICAL SOCIETYArticle Views8Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Biopolymers,Gene therapy,Immunology,Infectious diseases,Peptides and proteins Get e-Alerts
A high power and high efficiency monolithic power amplifier at V-band using pseudomorphic HEMTs (2002)

This paper presents a high power and high efficiency monolithic power amplifier at V-band utilizing highly reliable and manufacturable 0.15 /spl mu/m InGaAs/AlGaAs/GaAs pseudomorphic HEMT fabrication technology. The performance of the power amplifier is 13.83 dB small signal gain, 13.9% power-added-efficiency, and 26.83 dBm (482 mW) at 60 GHz for unpassivated HEMT process. The same circuit with passivated process produced a linear gain of 13.18 dB, 11% power-added-efficiency, and compressed output power of 25.68 dBm (370 mW). The producibility of this amplifier has been demonstrated in volume production with several wafer lots resulting in a 20% total yield through RF tests.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Niveaux glauconieux dans les sédiments récents du seuil nord-égéen (1975)

La formation de glauconie, sur le seuil Nord-Egéen, est permise par la présence de micro-milieux semi-confinés granulaires (tests de Foraminifères, granules de vase). On constate la dégradation des argiles initiales, corrélative d'un enrichissement en fer et de la formation de minéraux nouveaux qui se rapprochent d'une glauconite stricte. Ils demeurent à interstratifiés dominants, dont le terme essentiel est de type 10-14 5. Cette glauconie a été fossilisée lors de l'enfouissement. Le processus de glauco-nitisation a été particulièrement actif à deux reprises au cours de la sédimentation würmienne dans cette région.
Title Page, Table of Contents, & From the Editor (2015)

This semester’s edition of the Psi Sigma Siren showcases the hard work of several graduate students from the UNLV History program, and I believe their tireless dedication to the historian’s craft is reflected by their articles and book reviews. We received numerous submissions, and I am proud of the projects that were accepted for this issue.
Sustained regression of tumors upon MYC inactivation requires p53 or thrombospondin-1 to reverse the angiogenic switch (2006)

The targeted inactivation of oncogenes offers a rational therapeutic approach for the treatment of cancer. However, the therapeutic inactivation of a single oncogene has been associated with tumor recurrence. Therefore, it is necessary to develop strategies to override mechanisms of tumor escape from oncogene dependence. We report here that the targeted inactivation of MYC is sufficient to induce sustained regression of hematopoietic tumors in transgenic mice, except in tumors that had lost p53 function. p53 negative tumors were unable to be completely eliminated, as demonstrated by the kinetics of tumor cell elimination revealed by bioluminescence imaging. Histological examination revealed that upon MYC inactivation, the loss of p53 led to a deficiency in thrombospondin-1 (TSP-1) expression, a potent antiangiogenic protein, and the subsequent inability to shut off angiogenesis. Restoration of p53 expression in these tumors re-established TSP-1 expression. This permitted the suppression of angiogenesis and subsequent sustained tumor regression upon MYC inactivation. Similarly, the restoration of TSP-1 alone in p53 negative tumors resulted in the shut down of angiogenesis and led to sustained tumor regression upon MYC inactivation. Hence, the complete regression of tumor mass driven by inactivation of the MYC oncogene requires the p53-dependent induction of TSP-1 and the shut down of angiogenesis. Notably, overexpression of TSP-1 alone did not influence tumor growth. Therefore, the combined inactivation of oncogenes and angiogenesis may be a more clinically effective treatment of cancer. We conclude that angiogenesis is an essential component of oncogene addiction.
Examination for platelet-activating factor production by preimplantation mouse embryos using a specific radioimmunoassay (1990)

A specific and highly sensitive radioimmunoassay was used to measure platelet-activating factor (PAF) production by preimplantation mouse embryos in vitro. Levels of PAF greater than 1 pg per embryo were not observed in 24-h culture medium from 2-cell embryos, compacted morulae or blastocysts, or in extracts from these embryos. Synthetic PAF added to embryos at the start of culture could be almost totally recovered after the incubation period, indicating negligible degradation of PAF during culture. PAF was also not detected in embryo samples using a washed rabbit-platelet aggregation assay. It can be concluded that mouse embryos do not produce substantial levels of PAF, or any of the biologically active analogues of PAF detected by the assay.
A 120-mV input, fully integrated dual-mode charge pump in 65-nm CMOS for thermoelectric energy harvester (2012)

In this paper, a fully integrated low voltage charge pump for thermoelectric energy harvesters is presented. The proposed dual-mode architecture achieves both the low startup voltage in a startup mode and high conversion efficiency in a normal operation mode without off-chip inductors and capacitors. In the measurement, the proposed circuit successfully converts 120-mV input to 770-mV output with 38.8% conversion efficiency.
Measurement of the acoustic input immittance of the human ear (1981)

A method is presented for measuring acoustic immittance (admittance or impedance) in the human ear canal and its validity is demonstrated for frequencies from 62 Hz to 4 kHz. Special attention is given to estimating the residual ear-canal space between the eardrum and the tip of the measuring device; estimates were determined, in part, using immittances measured with static pressures applied in the ear canal. Immittance estimates at the eardrum are reported for four normal subjects. Below 500 Hz, the immittance is compliance dominated. Our averaged compliance equivalent to 0.70 cc agrees with that reported by others. From 1 to 4 kHz, the immittance is resistance dominated. Our resistance values exceed those generally reported previously; they are, however, similar to those of Mehrgardt and Mellert [J. Acoust. Soc. Am. 61, 1567--1576 (1977)] and to values suggested from analysis of other acoustical measurements on the external ear.
<sup>68</sup>Ga-DOTA-Tyr<sup>3</sup>-Octreotide PET for Assessing Response to Somatostatin-Receptor–Mediated Radionuclide Therapy (2009)

(68)Ga-DOTA-TOC PET shows no advantage over conventional anatomic imaging for assessing response to therapy when all CT information obtained during follow-up is compared. Only the development of new metastases during therapy was detected earlier in some cases when whole-body PET was used. SUV analysis of individual lesions is of no additional value in predicting individual responses to therapy.
A systematic review and meta-analysis: probiotics in the treatment of irritable bowel syndrome (2009)

Probiotics may have a role in alleviating some of the symptoms of IBS, a condition for which currently evidence of efficacy of drug therapies is weak. However, as IBS is a condition that is chronic and usually intermittent longer term trials are recommended. Such research should focus on the type, optimal dose of probiotics and the subgroups of patients who are likely to benefit the most.
Regulation of protein synthesis in rabbit reticulocyte lysates: purification and characterization of heme-reversible translational inhibitor. (1978)

To define the mechanism of regulation of the protein kinase that is activated in heme deficiency and that inhibits initiation of protein synthesis, we have isolated and purified the heme-reversible form of the protein kinase from rabbit reticulocytes. The inhibitory activity is found in a single band after polyacrylamide gel electrophoresis under nondenaturing conditions. It migrates as a 95,000-dalton polypeptide in 15% sodium dodecyl sulfate/polyacrylamide gels. This purified inhibitor becomes self-phosphorylated in the presence of ATP; the phosphorylated protein and the inhibitory activity copurify. The inhibitor produces characteristic biphasic kinetics of inhibition in reticulocyte lysates and phosphorylates the 38,000-dalton subunit of eukaryotic initiation factor 2 (eIF-2); the inhibition is reversed by added eIF-2. In contrast to the heme-irreversible inhibitor, this heme-reversible inhibitor is no longer inhibitory after incubation with 20 micron hemin. Incubation with hemin also inhibits self-phosphorylation. Preincubation of the heme-reversible inhibitor in the presence of ATP potentiates the inhibition of protein synthesis in the subsequent incubation, as does treatment with N-ethylmaleimide. Phosphorylation of the heme-reversible inhibitor and inhibition of protein synthesis in the lysate due to phosphorylation of eIF-2 appear to be related. These findings suggest that hemin acts directly on the heme-reversible inhibitor.
Schiff-Base Metal(II) Complexes as New Catalysts in the Efficient, Mild and Regioselective Conversion of 1,2-Epoxyethanes to 2-Hydroxyethyl Thiocyanates with Ammonium Thiocyanate (2003)

Abstract A convenient and efficient procedure for the ring-opening of 1,2- epoxyethanes with ammonium thiocyanate in the presence of some Schiff-base complexes of metal(II) is described. The cleavage of the oxirane ring is found to proceed regioselectively under mild reaction conditions. Thus, several 2-hydroxyethyl thiocyanates, useful intermediates toward biological-active molecules, are easily obtained in very good yields.
Activation of manganese dioxide by azeotropic removal of water (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTActivation of manganese dioxide by azeotropic removal of waterI. M. GoldmanCite this: J. Org. Chem. 1969, 34, 6, 1979–1981Publication Date (Print):June 1, 1969Publication History Published online1 May 2002Published inissue 1 June 1969https://pubs.acs.org/doi/10.1021/jo01258a106https://doi.org/10.1021/jo01258a106research-articleACS PublicationsRequest reuse permissionsArticle Views4155Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Technical Reference on Hydrogen Compatibility of Materials: Aluminum Alloys: Non-Heat Treatable Alloys Pure Al (code 3001). (2020)

Abstract not provided.
Multifunctional HER2-Antibody Conjugated Polymeric Nanocarrier-Based Drug Delivery System for Multi-Drug-Resistant Breast Cancer Therapy (2014)

Nanotechnology-based medical approaches have made tremendous potential for enhancing the treatment efficacy with minimal doses of chemotherapeutic drugs against cancer. In this study, using tamoxifen (Tam), biodegradable antibody conjugated polymeric nanoparticles (NPs) was developed to achieve targeted delivery as well as sustained release of the drug against breast cancer cells. Poly(D,L-lactic-co-glycolic acid) (PLGA) NPs were stabilized by coating with poly(vinyl alcohol) (PVA), and copolymer polyvinyl-pyrrolidone (PVP) was used to conjugate herceptin (antibody) with PLGA NPs for promoting the site-specific intracellular delivery of Tam against HER2 receptor overexpressed breast cancer (MCF-7) cells. The Tam-loaded PVP-PLGA NPs and herceptin-conjugated Tam-loaded PVP-PLGA NPs were characterized in terms of morphology, size, surface charge, and structural chemistry by dynamic light scattering (DLS), Transmission electron microscopy (TEM), ζ potential analysis, 1H nuclear magnetic resonance (NMR), and Fourier transform infrared (FT-IR) spectroscopy. pH-based drug release property and the anticancer activity (in vitro and in vivo models) of the herceptin conjugated polymeric NPs were evaluated by flow cytometry and confocal image analysis. Besides, the extent of cellular uptake of drug via HER2 receptor-mediated endocytosis by herceptin-conjugated Tam-loaded PVP-PLGA NPs was examined. Furthermore, the possible signaling pathway of apoptotic induction in MCF-7 cells was explored by Western blotting, and it was demonstrated that drug-loaded PLGA NPs were capable of inducing apoptosis in a caspase-dependent manner. Hence, this nanocarrier drug delivery system (DDS) not only actively targets a multidrug-resistance (MDR) associated phenotype (HER2 receptor overexpression) but also improves therapeutic efficiency by enhancing the cancer cell targeted delivery and sustained release of therapeutic agents.