gebhart/patrepeval huggingface dataset:
Serological studies of a host range mutant of a lactic streptococcal bacteriophage (1978)

A host range mutant was isolated from a bacteriophage that attacked Streptococcus cremoris 114. The mutant was able to adsorb and grow on S. cremoris 266, where the parent phage could not. The mutant phage was unable to adsorb to the original bacterial host, S. cremoris 114. The change in host range was accompanied by an alteration in the neutralization antigen as shown by a change in neutralization rate by an anti-phage serum. Serum-blocking experiments confirmed the difference in neutralization antigen between parent and mutant phages. The two phages nevertheless had similar complement fixation antigens, confirming that one was a mutant derived from the other. A distinction between complement fixation and neutralization antigens, similar to that found for the coliphages and staphylococcal phages, has therefore been demonstrated for two lactic streptococcal phages.
Changes in Asphaltene Properties during Hydrotreating of Heavy Crudes (2003)

Precipitation with n-heptane and characterization of asphaltenes were carried out for products obtained during hydrotreating of Maya heavy crude oil. Hydrotreating experiments were conducted in a pilot plant at constant pressure, hydrogen-to-oil ratio, and space-velocity. Reaction temperature was varied in the range of 380−440 °C. Maya crude and hydrotreated products were characterized by API gravity and sulfur, nitrogen, nickel, vanadium, and asphaltenes contents. Elemental analysis, metals content, VPO apparent molecular weight, and NMR measurements were performed in the asphaltenes fraction. The effects of reaction temperature on asphaltenes properties during hydrotreating are discussed in terms of changes in heteroatoms contents and structural parameters.
Interaction of tubulin with bifunctional colchicine analogs: an equilibrium study (1984)

The interaction of tubulin with simple analogues of colchicine that contain both its tropolone and trimethoxyphenyl rings has been characterized, and the results were analyzed in terms of the simple bifunctional ligand model developed for the binding of colchicine [ Andreu , J. M., & Timasheff , S. N. (1982) Biochemistry 21, 534-543] on the basis of interactions of tubulin with single-ring analogues. The compound 2-methoxy-5-(2,3,4-trimethoxyphenyl)-2,4,6- cycloheptatrien -1-one has been found to bind reversibly to 0.86 +/- 0.06 site of purified calf brain tubulin with an equilibrium constant of (4.9 +/- 0.3) X 10(5) M-1 (25 degrees C), delta H degrees app = -1.6 +/- 0.7 kcal mol-1, and delta S degrees app = 20.5 +/- 2.5 eu. The binding appears specific for the colchicine site. The closely related compound 2-methoxy-5-[[3-(3,4,5-trimethoxyphenyl)-propionyl]amino] -2,4,6- cycloheptatrien -1-one interacts weakly with tubulin. Binding of the first analogue is accompanied by ligand fluorescence appearance, quenching of protein fluorescence, perturbation of the far-ultraviolet circular dichroism of tubulin, and induction of the tubulin GTPase activity, similarly to colchicine binding. Substoichiometric concentrations of the analogue inhibit microtubule assembly in vitro. Excess analogue concentration under microtubule-promoting conditions induces an abnormal cooperative polymerization of tubulin, similar to that of the tubulin-colchicine complex.
One-Pot Synthesis of Chemically Modified Vegetable Oils (2008)

Vegetable oils are promising candidates as substitutes for petroleum base oils in lubricant applications, such as total loss lubrication, military applications, and outdoor activities. Although vegetable oils have some advantages, they also have poor oxidation and low temperature stability. One of the ways to address these issues is chemical modification of fatty acid chain of triglyceride. We report a one-pot synthesis of a novel class of chemically modified vegetable oils from epoxidized triacylglycerols and various anhydrides. In an anhydrous solvent, boron trifluoride etherate is used as catalyst to simultaneously open the oxirane ring and activate the anhydride. The reaction was monitored and products confirmed by NMR, FTIR, GPC, and TGA analysis. Experimental conditions were optimized for research quantity and laboratory scale-up (up to 4 lbs). The resultant acyl derivatives of vegetable oil, having diester substitution at the sites of unsaturation, have potential in formulation of industrial fluids such as hydraulic fluids, lubricants, and metal working fluids.
Road detection using segmentation by weighted aggregation based on visual information and a posteriori probability of road regions (2008)

In this paper, a road detection method based on an image segmentation algorithm is presented. Road detection process is a key issue for an autonomous driving system in urban environment. Image-based road detection algorithm is applied on sources of visual information recorded by cameras when our car is running on road. Our method combines a posteriori probability and visual information for image segmentation. The method is composed of two steps. Firstly, a road identifier is trained with supervised learning algorithm. Secondly, road regions are detected by combining a posteriori probability and visual information using image segmentation algorithm. Experimental results are presented for road images in actual driving video acquired in urban areas.
On-Line Data Reconstruction in Redundant Disk Arrays (CMU-CS-94-164) (2018)

There exists a wide variety of applications in which data availability must be continuous, that is, where the system is never taken off-line and any interruption in the accessibility of stored data causes significant disruption in the service provided by the application. Examples include on-line transaction processing systems such as airline reservation systems, and automated teller networks in banking systems. In addition, there exist many applications for which a high degree of data availability is important, but continuous operation is not required. An example is a research and development environment, where access to a centrally-stored CAD system is often necessary to make progress on a design project. These applications and many others mandate both high performance and high availability from their storage subsystems. Parity-based redundant disk arrays are very attractive storage alternatives for these systems because they offer both low cost per megabyte and high data reliability. Unfortunately such systems exhibit poor availability characteristics; their performance is severely degraded in the presence of a disk failure. This dissertation addresses the design of parity-based redundant disk arrays that offer dramatically higher levels of performance in the presence of failure than systems comprising the current state of the art. We consider two primary aspects of the failure-recovery problem: the organization of the data and redundancy in the array, and the algorithm used to recover the lost data. We apply results from combinatorial theory to generate data and parity organizations that minimize performance degradation during failure recovery by evenly distributing all failure-induced workload over a larger-than-minimal collection of disks. We develop a reconstruction algorithm that is able to absorb for failure-recovery essentially all of the array's bandwidth that is not absorbed by the application process(es). Additionally, we develop a design for a redundant disk array targeted at extremely high availability through extremely fast failure recovery. This development also demonstrates the generality of the presented techniques.
Human Carbonyl Reductase 1 Is an S-Nitrosoglutathione Reductase (2008)

Human carbonyl reductase 1 (hCBR1) is an NADPH-dependent short chain dehydrogenase/reductase with broad substrate specificity and is thought to be responsible for the in vivo reduction of quinones, prostaglandins, and other carbonyl-containing compounds including xenobiotics. In addition, hCBR1 possesses a glutathione binding site that allows for increased affinity toward GSH-conjugated molecules. It has been suggested that the GSH-binding site is near the active site; however, no structures with GSH or GSH conjugates have been reported. We have solved the x-ray crystal structures of hCBR1 and a substrate mimic in complex with GSH and the catalytically inert GSH conjugate hydroxymethylglutathione (HMGSH). The structures reveal the GSH-binding site and provide insight into the affinity determinants for GSH-conjugated substrates. We further demonstrate that the structural isostere of HMGSH, S-nitrosoglutathione, is an ideal hCBR1 substrate (Km = 30 microm, kcat = 450 min(-1)) with kinetic constants comparable with the best known hCBR1 substrates. Furthermore, we demonstrate that hCBR1 dependent GSNO reduction occurs in A549 lung adenocarcinoma cell lysates and suggest that hCBR1 may be involved in regulation of tissue levels of GSNO.
Net-dbx-G: A web-based debugger of MPI programs over grid environments (2004)

Net-dbx-G is a tool that utilizes Java and other World Wide Web tools as an interface to Grid services to help Grid application developers debug their MPI programs from anywhere in the Internet. Net-dbx-G is a source level debugger with the full power of gdb (the GNU Debugger), providing the user with full Grid functionality. The portability of the tool is of great importance as well because it enables the tool to be used on heterogeneous nodes that participate in an MPI enabled, Grid environment. The users of our system simply point their browser to the Net-dbx-G webpage and authenticate to the virtual organization that they are part of. Having at their disposal the shared resources participating with that VO, they start debugging by interacting with the provided GUI environment of the tool. The users can dynamically select which MPI processes to view/debug.
Effect of fatigue on joint position sense of the knee (1986)

This study was designed to test position sense of the knee joint before and after fatigue in order to determine whether muscle or capsular receptors are the primary sensors for joint position sense. Reproduction of passive positioning and detection of the onset of motion (kinesthesia) were employed to measure joint position sense. Eleven subjects underwent joint position sense measurement before and after a fatigue protocol. A significant worsening of reproduction of knee joint angle after fatigue was noted (p less than 0.05). Threshold (kinesthesia) showed no statistically significant change after fatigue. A significant correlation of reproduction measurements and threshold measurements prior to fatigue (p less than 0.01) demonstrated that the same neural mechanism is applicable in the rested state, but these variables did not correlate significantly after fatigue. There was a significant correlation between reproduction measurements before and after fatigue (p = 0.018), while no correlation was seen for the pre- and postfatigue threshold measurements, suggesting a change in the neural path after fatigue. Since both tests of joint position sense are affected by fatigue, we conclude that muscle receptors are a prominent, if not primary, determinant of joint position sense, and capsular receptors may have a secondary role. Reproduction ability is decreased, presumably through the loss of efficiency of muscle receptors. The threshold data suggest a change in the mechanism of appreciation after fatigue, possibly due to increased sensitivity of capsular receptors from muscle-fatigue-induced laxity.
A New Design of a Two-Stage Cycloidal Speed Reducer (2011)

A new design of a two-stage cycloidal speed reducer is presented in this paper. A traditional two-stage cycloidal speed reducer is obtained by the simple combination of single-stage cycloidal speed reducers. A single-stage reducer engages two identical cycloid discs in order to balance dynamical loads and to obtain uniform load distribution. Consequently, the traditional two-stage reducer has four cycloid discs, in total. The newly designed two-stage cycloidal speed reducer, presented in this paper, has one cycloid disc for each stage, that is, two cycloid discs in total, which means that it is rather compact. Due to its specific concept, this reducer is characterized by good load distribution and dynamic balance, and this is described in the paper. Stress state analysis of cycloidal speed reducer elements was also realized, using the finite elements method (FEM), for the most critical cases of conjugate gear action (one, two, or three pairs of teeth in contact). The results showed that cycloid discs are rather uniformly loaded, justifying the design solution presented here. Experimental analysis of the stress state for cycloid discs was realized, using the strain gauges method. It is easy to conclude, based on the obtained results, that even for the most critical case (one pair of teeth in contact) stresses on cycloid discs are in the allowed limits, thus providing normal functioning of the reducer for its anticipated lifetime.
GC‐MS analysis of penta‐ and tetra‐cyclic triterpenes from resins of <i>Pistacia</i> species. Part I. <i>Pistacia lentiscus</i> var. Chia (2005)

Pistacia species contain oleoresins with bioactive triterpenes. In this study triterpenes, including minor components, were identified and quantified in both neutral and acidic fractions of Pistacia terebinthus var. Chia resin, grown exclusively in Chios island (Greece), collected traditionally, as well as using stimulating agents (liquid collection). It was proved that these two resin samples were composed of several different minor triterpenes, while major constituents were similar but in different proportions. Compounds that differentiated two resin samples of P. lentiscus and P. terebinthus var. Chia, both traditionally collected, were detected, in order to identify the nature of resins present in archaeological materials. In the traditionally collected resin, 37 triterpenes were identified, 12 in the acidic and 25 in the neutral fraction. In the liquid collection resin 10 compounds were identified in the acidic and 23 in the neutral fraction, while 16 compounds were not contained in the traditionally collected resin. The main triterpenes in both resin samples collected traditionally and using stimulating agents were: isomasticadienonic acid (23.6 and 26.3% w[sol ]w of the triterpenic fraction, respectively), 28-norolean-17-en-3-one (16.3 and 17.5% w[sol ]w of the triterpenic fraction, respectively) and masticadienonic acid (5.8 and 6.0% w[sol ]w of the triterpenic fraction). In this study the qualitative and quantitative composition of triterpenes was compared in the Pistacia lentiscus and P. terebinthus var. Chia resin samples collected with the traditional and new liquid techniques, and also triterpenes in resins of P. terebinthus obtained by the traditional technique and using stimulating agents. The aim of the study was also to examine whether the collection technique influenced the triterpenes contained in P. terebinthus var. Chia resin samples.
A technical framework for sense-and-respond business management (2005)

In this paper we present a technical framework that supports sense and respond (SaR), the approach that enables an enterprise to adapt to a rapidly changing business environment. To implement the SaR approach, an enterprise proactively monitors trends and uses effective decision-support tools to help it act in a timely manner. We describe two pilot projects in which we implemented SaR prototypes and applied them to solve business problems. In the first pilot project we helped the IBM Microelectronics Division deploy an automated inventory management system based on our inventory optimization model. In the second pilot project, we helped the IBM Personal Computing Division deploy a SaR system in support of demand/supply conditioning. One of the components of this SaR system is an order trend model that provides early warning of constraints and excesses in the supply chain and helps make demand/supply conditioning more effective. Early results from these projects are encouraging and show that significant gains in profitability are possible.
Hydrozirconation. III. Stereospecific and regioselective functionalization of alkylacetylenes via vinylzirconium(IV) intermediates (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrozirconation. III. Stereospecific and regioselective functionalization of alkylacetylenes via vinylzirconium(IV) intermediatesDonald W. Hart, Thomas F. Blackburn, and Jeffrey SchwartzCite this: J. Am. Chem. Soc. 1975, 97, 3, 679–680Publication Date (Print):February 1, 1975Publication History Published online1 May 2002Published inissue 1 February 1975https://pubs.acs.org/doi/10.1021/ja00836a056https://doi.org/10.1021/ja00836a056research-articleACS PublicationsRequest reuse permissionsArticle Views2532Altmetric-Citations242LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Improvement of Depth Profiling into Biotissues Using Micro Electrical Impedance Spectroscopy on a Needle with Selective Passivation (2016)

A micro electrical impedance spectroscopy (EIS)-on-a-needle for depth profiling (μEoN-DP) with a selective passivation layer (SPL) on a hypodermic needle was recently fabricated to measure the electrical impedance of biotissues along with the penetration depths. The SPL of the μEoN-DP enabled the sensing interdigitated electrodes (IDEs) to contribute predominantly to the measurement by reducing the relative influence of the connection lines on the sensor output. The discrimination capability of the μEoN-DP was verified using phosphate-buffered saline (PBS) at various concentration levels. The resistance and capacitance extracted through curve fitting were similar to those theoretically estimated based on the mixing ratio of PBS and deionized water; the maximum discrepancies were 8.02% and 1.85%, respectively. Depth profiling was conducted using four-layered porcine tissue to verify the effectiveness of the discrimination capability of the μEoN-DP. The magnitude and phase between dissimilar porcine tissues (fat and muscle) were clearly discriminated at the optimal frequency of 1 MHz. Two kinds of simulations, one with SPL and the other with complete passivation layer (CPL), were performed, and it was verified that the SPL was advantageous over CPL in the discrimination of biotissues in terms of sensor output.
Effects of deliberate copper contamination from the plating solution on the electrical characteristics of MOSFETs (2001)

N-channel and p-channel metal-oxide-semiconductor (MOS) transistors of various (W/L) ratios down to 0.24-/spl mu/m channel length have been used to investigate the effects of deliberate backside copper (Cu) contamination on the MOS field-effect transistor (MOSFET) electrical parameters. The backside of the wafer was flooded with copper sulphate (CuSO/sub 4/) solution and air-dried. High-temperature annealing was carried out to drive Cu into silicon. It was discovered that the backside Cu contamination did not result in any undesirable effects on the MOS device performance. The MOS device parameters such as threshold voltage V/sub TO/, transconductance G/sub m/, drain saturation current I/sub DSAT/, off-current I/sub off/, and junction leakage current for n/sup +//p and p+/n diodes displayed no significant degradation, even after 5 h of annealing at 400/spl deg/C in nitrogen ambient. Secondary ion mass spectroscopy data shows that Cu diffused into silicon only over a short distance, leading to little or no degradation of MOSFETs and junction diodes.
Generation of Ugt1-Deficient Murine Liver Cell Lines Using TALEN Technology (2014)

The Crigler-Najjar Syndrome Type I (CNSI) is a rare genetic disorder caused by mutations in the Ugt1a1 gene. It is characterized by unconjugated hyperbilirubinemia that may result in severe neurologic damage and death if untreated. To date, liver transplantation is the only curative treatment. With the aim of generating mutant cell lines of the Ugt1 gene, we utilized the TALEN technology to introduce site-specific mutations in Ugt1 exon 4. We report a fast and efficient method to perform gene knockout in tissue culture cells, based on the use of TALEN pairs targeting restriction enzyme (RE) sites in the region of interest. This strategy overcame the presence of allele-specific single nucleotide polymorphisms (SNPs) and pseudogenes, conditions that limit INDELs' detection by Surveyor. We obtained liver-derived murine N-Muli cell clones having INDELs with efficiency close to 40%, depending on the TALEN pair and RE target site. Sequencing of the target locus and WB analysis of the isolated cell clones showed a high proportion of biallelic mutations in cells treated with the most efficient TALEN pair. Ugt glucuronidation activity was reduced basal levels in the biallelic mutant clones. These mutant liver-derived cell lines could be a very useful tool to study biochemical aspects of Ugt1 enzyme activity in a more natural context, such as substrate specificity, requirement of specific co-factors, the study of inhibitors and other pharmacological aspects, and to correlate enzyme activity to the presence of specific mutations in the gene, by adding back to the mutant cell clones specific variants of the Ugt1 gene. In addition, since genome editing has recently emerged as a potential therapeutic approach to cure genetic diseases, the definition of the most efficient TALEN pair could be an important step towards setting up a platform to perform genome editing in CNSI.
Broadband planar millimeter wave dipole with flip-chip interconnect (2007)

In this paper a broadband planar dipole superstrate antenna suitable for integration with millimeter wave (MMW) transceiver ICs is presented. The dipole is printed on the bottom of a fused silica substrate with a ground plane below. This enables packaging together with a transceiver IC using flip-chip interconnect technology. A 60GHz dual dipole array was flip-chip mounted onto a coplanar waveguide (CPW) thru line to evaluate the antenna design together with the flip-chip interconnect. In the following measured antenna return loss and far field radiation pattern will be shown and discussed.
Comparison of titanium-tantalum and titanium-niobium alloys for application as dental implants. (1989)

For the diffusion bonding of Ti alloys to alumina, materials with a similar thermal expansion coefficient to alumina are required. Such materials were developed by alloying the biocompatible elements Ta and Nb to titanium (Ti-Ta30 and Ti-Ta40, Ti-Nb40 and Ti-Nb50). While measuring the corrosion behavior of these alloys in 0.9% saline solution, no difference could be observed. The Ti-Ta alloys are superior in their mechanical properties to Ti-Nb alloys.