gebhart/patrepeval huggingface dataset:
Backscattering Raman spectroscopy using multi-grating spatial heterodyne Raman spectrometer (2018)

Spatial heterodyne Raman spectrometry (SHRS) is a spectral analysis technique used to study material structures and compositions. We propose a multi-grating SHRS system that uses a multi-grating module rather than the single grating used to terminate each arm in traditional spatial heterodyne spectrometry (SHS). The proposed system not only retains the advantages of traditional SHS but also resolves the mutual limitation between system spectral range and resolution. The increased spectral range and resolution that can be achieved in detection are dependent on the number of sub-gratings used in the module. A verification system was built using 130 gr/mm and 150 gr/mm sub-gratings and calibrated. Under different experimental conditions (including laser power, integration time, container material and thickness, pure and mixed samples, and standoff experiments), the backscattered Raman spectra of different types of targets (including organic solutions, inorganic powders, and minerals) were tested. The multi-grating SHRS shows good performance for broad spectral range and high-resolution Raman detection.
Lack of Enhancer Function in Mammals Is Unique to Oocytes and Fertilized Eggs (1999)

Previous studies have shown that the lack of novel coactivator activity in mouse oocytes and one-cell embryos (fertilized eggs) renders them incapable of utilizing Gal4:VP16-dependent enhancers (distal elements) but not promoters (proximal elements) in regulating transcription. This coactivator activity first appears in two- to four-cell embryos coincident with the major activation of zygotic gene expression. Here we show that whereas oocytes and fertilized eggs could utilize Sp1-dependent promoters, they could not utilize Sp1-dependent enhancers, although they showed promoter repression, which is a requirement for delineating enhancer function. In contrast, both Sp1-dependent promoters and enhancers were functional in two- to four-cell embryos. Furthermore, the same embryonic stem cell mRNA that provided the coactivator activity for Gal4:VP16-dependent enhancer function also provided Sp1-dependent enhancer function in oocytes. Therefore, the coactivator activity appears to be a requirement for general enhancer function. To determine whether the absence of enhancer function is a unique property of oocytes or a general property of other terminally differentiated cells, transcription was examined in terminally differentiated hNT neurons and their precursors, undifferentiated NT2 stem cells. The results showed that both cell types could utilize enhancers and promoters. Thus, in mammals, the lack of enhancer function appears to be unique to oocytes and fertilized eggs, suggesting that it provides a safeguard against premature activation of genes prior to zygotic gene expression during development.
Effects of <i>S</i> -Nitrosation of Hemoglobin on Hypoxic Pulmonary Vasoconstriction and Nitric Oxide Flux (2001)

Free hemoglobin (Hb) augments hypoxic pulmonary vasoconstriction (HPV), ostensibly by scavenging nitric oxide (NO). However, recent evidence suggests that Hb that is S-nitrosated may act as an NO donor and vasodilator. We studied the effects of oxyHb, Hb that is chemically modified to prevent heme binding or oxidation of NO (cyanometHb), and Hb that is S-nitrosated (SNO-Hb and SNO-cyanometHb) on HPV, expired NO (eNO), and perfusate S-nitrosothiol (SNO) concentration in isolated, perfused rabbit lungs. Perfusate containing either 4 microM oxyHb or SNO-Hb increased normoxic pulmonary artery pressure (Ppa), augmented HPV dramatically, and resulted in an 80% fall in eNO in comparison to perfusion with buffer, whereas 4 microM cyanometHb or SNO-cynanometHb had no effect on these variables. Excess glutathione (GSH) added to perfusate containing SNO-Hb resulted in a 20 to 40% fall in the perfusate SNO concentration, with a concomitant increase in metHb content, without affecting Ppa, HPV, or eNO. In conclusion, free Hb augments HPV by scavenging NO, an effect that is not prevented by S-nitrosation. NO released from SNO-Hb in the presence of GSH does not produce measurable vascular effects in the lung or changes in eNO because of immediate oxidation and metHb formation.
Anti-inflammatory Effect of Diclofenac Drops After Argon Laser Trabeculoplasty (1993)

Using a laser flare-cell meter, the anti-inflammatory effect of diclofenac sodium drops was evaluated after argon laser trabeculoplasty in a double-masked placebo-controlled study. Fifty-three eyes with pseudoexfoliative (43 eyes) or pigmentary glaucoma (10 eyes) were randomly assigned to treatment (n = 27) or placebo (n = 26) groups. Diclofenac (0.1%) or placebo drops were administered once before and after trabeculoplasty and then four times daily for a total of 4 days. Visual acuity, intraocular pressure, and anterior chamber flare, measured with the laser flare-cell meter, were evaluated before trabeculoplasty, 3 and 6 hours after trabeculoplasty, and 1, 2, 4, 7, and 14 days after trabeculoplasty. Flare increase after argon laser trabeculoplasty was completely blocked by 0.1% topical diclofenac and the mean of maximal flare increases was reduced significantly in the diclofenac group. Therefore, 0.1% diclofenac drops represent a very efficient anti-inflammatory therapy after argon laser trabeculoplasty.
Creating a network for interactivity (1995)

The one most likely system, to serve as the common meeting ground for phone and cable companies, is a hybrid network architecture of optical fibers and coaxial cables. As the industry evolves toward interactive services, three considerations will become paramount. One is the ability to deliver individualized programming to customers, and get responses from them as well. Here, the instantaneous network bandwidth is of less concern than the effective bandwidth per customer. So long as all the programming and services a customer desires are available in a user-friendly way, it is irrelevant how the network delivers them. By its nature, interactivity also implies that the measure of effective bandwidth per customer be extended to bidirectional communication between the customer and the head-end. Several ways of providing that increased effective bandwidth are discussed. A second prime concern is the need for more attention to network reliability in delivering services. Finally, the issues of standards and security must be dealt with.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Da Suécia: Mais sôbre o "Fartlek" (1956)

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Stereospecific Cross-Coupling of Secondary Organotrifluoroborates: Potassium 1-(Benzyloxy)alkyltrifluoroborates (2012)

Potassium 1-(alkoxy/acyloxy)alkyltrifluoroborates have been synthesized through a copper-catalyzed diboration of aldehydes and subsequent conversion of the resulting potassium 1-(hydroxy)alkyltrifluoroborates. The palladium-catalyzed Suzuki-Miyaura reaction employing the potassium 1-(benzyloxy)alkyltrifluoroborates with aryl and heteroaryl chlorides provides access to protected secondary alcohols in high yields. The β-hydride elimination pathway is avoided through use of the benzyl protecting group, which is proposed to stabilize the diorganopalladium intermediate by coordination of the arene to the metal center. This cross-coupling is stereospecific with complete retention of stereochemistry.
Ribonucleic acid ligase activity of deoxyribonucleic acid ligase from phage T4 infected Escherichia coli (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRibonucleic acid ligase activity of deoxyribonucleic acid ligase from phage T4 infected Escherichia coliHiroshi Sano and Guenter FeixCite this: Biochemistry 1974, 13, 25, 5110–5115Publication Date (Print):December 1, 1974Publication History Published online1 May 2002Published inissue 1 December 1974https://pubs.acs.org/doi/10.1021/bi00722a009https://doi.org/10.1021/bi00722a009research-articleACS PublicationsRequest reuse permissionsArticle Views61Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Accuracy of finite element model‐based multi‐organ deformable image registration (2005)

As more pretreatment imaging becomes integrated into the treatment planning process and full three-dimensional image-guidance becomes part of the treatment delivery the need for a deformable image registration technique becomes more apparent. A novel finite element model-based multiorgan deformable image registration method, MORFEUS, has been developed. The basis of this method is twofold: first, individual organ deformation can be accurately modeled by deforming the surface of the organ at one instance into the surface of the organ at another instance and assigning the material properties that allow the internal structures to be accurately deformed into the secondary position and second, multi-organ deformable alignment can be achieved by explicitly defining the deformation of a subset of organs and assigning surface interfaces between organs. The feasibility and accuracy of the method was tested on MR thoracic and abdominal images of healthy volunteers at inhale and exhale. For the thoracic cases, the lungs and external surface were explicitly deformed and the breasts were implicitly deformed based on its relation to the lung and external surface. For the abdominal cases, the liver, spleen, and external surface were explicitly deformed and the stomach and kidneys were implicitly deformed. The average accuracy (average absolute error) of the lung and liver deformation, determined by tracking visible bifurcations, was 0.19 (s.d.: 0.09), 0.28 (s.d.: 0.12) and 0.17 (s.d.: 0.07) cm, in the LR, AP, and IS directions, respectively. The average accuracy of implicitly deformed organs was 0.11 (s.d.: 0.11), 0.13 (s.d.: 0.12), and 0.08 (s.d.: 0.09) cm, in the LR, AP, and IS directions, respectively. The average vector magnitude of the accuracy was 0.44 (s.d.: 0.20) cm for the lung and liver deformation and 0.24 (s.d.: 0.18) cm for the implicitly deformed organs. The two main processes, explicit deformation of the selected organs and finite element analysis calculations, require less than 120 and 495 s, respectively. This platform can facilitate the integration of deformable image registration into online image guidance procedures, dose calculations, and tissue response monitoring as well as performing multi-modality image registration for purposes of treatment planning.
Rapid antigen detection testing in diagnosing group A β-hemolytic streptococcal pharyngitis (2006)

Group A beta-hemolytic streptococcus (GABHS) is the most common bacterial cause of acute pharyngitis. Clinical criteria alone are not reliable enough to diagnose GABHS pharyngitis. Microbiological-testing is required for correct diagnosis. Although a throat swab culture remains the gold standard for documenting the presence of GABHS, a significant disadvantage of the culture is the delayed time of 1-2 days to obtain results. Most rapid antigen detection tests can provide results in less than 15 min. Rapid identification and treatment of patients with GABHS pharyngitis can reduce the risk of the spread of disease, may shorten the duration of symptoms, decrease the incidence of suppurative complications, decrease the amount of time lost from school/work, decrease the inappropriate use of antibiotics, reduce patient/parent dissatisfaction and alleviate the need for costly follow-up visits. All rapid antigen detection tests involve extraction of the group-specific carbohydrate antigen from the GABHS cell wall and identification of the antigen by an immunological reaction. There are numerous rapid antigen detection testing methods, namely latex agglutination, enzyme immunoassay, optical immunoassay, chemiluminescent DNA probes and PCR methods. Most of the rapid antigen detection tests that are currently in use have an excellent specificity of greater than 95% and a sensitivity of greater than or equal to 90%. Owing to the high specificity of the rapid antigen detection tests, a positive rapid antigen detection test is accepted as adequate for the diagnosis of GABHS pharyngitis. Conversely, confirmation of a negative antigen detection test with a throat culture result is necessary, unless the physician has ascertained in his/her practice that the sensitivity of the rapid antigen test used is comparable with that of a throat culture.
Increase of open‐cell content by plasticizing soft regions with secondary blowing agent (2005)

Abstract This article describes the effects of n‐butane mixed with primary CO 2 as a secondary blowing agent on the cell‐population density, the volume expansion ratio, and the open‐cell content of low‐density polyethylene (LDPE) and LDPE/polystyrene (PS) blends in extrusion. With the plasticizing effect of n‐butane, a high open‐cell content (up to 100%) over a wide range of processing temperatures was successfully achieved. POLYM. ENG. SCI., 45:1445–1451, 2005. © 2005 Society of Plastics Engineers
Structure and expression of a 72-kDa regulatory subunit of protein phosphatase 2A. Evidence for different size forms produced by alternative splicing (1993)

The trimeric form of protein phosphatase 2A consisting of 36-, 65-, and 72-kDa subunits (previously termed polycation-stimulated protein phosphatase M) was purified from rabbit skeletal muscle. Amino acid sequence data of the 72-kDa regulatory subunit (termed PR72) were used to isolate cDNAs from human heart and fetal brain libraries and libraries derived from WI-38 and MCF-7 cells. The clones isolated from the heart cDNA library revealed an open reading frame encoding a protein with a predicted molecular mass of 62 kDa. All the peptides sequenced from the protein matched with the sequence predicted from the cDNA. However, in vitro transcription and translation from this cDNA yielded a protein with an apparent molecular mass of 72 kDa on sodium dodecyl sulfate-polyacrylamide gels. From brain we isolated cDNA clones spanning an open reading frame encoding a 130-kDa protein (termed PR130). The apparent molecular mass of the protein produced by in vitro transcription and translation was 130 kDa. This protein has exactly the same deduced C-terminal protein sequence as the PR72 subunit from amino acids 45 to 527 but has an N-terminal extension of 665 amino acids. It is likely, therefore, that these two proteins arise from the same gene by alternative splicing. In human tissues several transcripts were detected by Northern analysis generated probably by the use of different polyadenylation signals and alternative splicing. High levels of the PR72 mRNAs were detected in heart and muscle, while lower levels of PR130 transcripts were found in heart, brain, placenta, lung, muscle, and kidney.
Generation and Analysis of Mice Lacking the Chemokine Fractalkine (2001)

Fractalkine (CX(3)CL1) is the first described chemokine that can exist either as a soluble protein or as a membrane-bound molecule. Both forms of fractalkine can mediate adhesion of cells expressing its receptor, CX(3)CR1. This activity, together with its expression on endothelial cells, suggests that fractalkine might mediate adhesion of leukocytes to the endothelium during inflammation. Fractalkine is also highly expressed in neurons, and its receptor, CX(3)CR1, is expressed on glial cells. To determine the biologic role of fractalkine, we used targeted gene disruption to generate fractalkine-deficient mice. These mice did not exhibit overt behavioral abnormalities, and histologic analysis of their brains did not reveal any gross changes compared to wild-type mice. In addition, these mice had normal hematologic profiles except for a decrease in the number of blood leukocytes expressing the cell surface marker F4/80. The cellular composition of their lymph nodes did not differ significantly from that of wild-type mice. Similarly, the responses of fractalkine(-/-) mice to a variety of inflammatory stimuli were indistinguishable from those of wild-type mice.
Adsorption and Chromatographic Separation of Mo(VI) and Zr(IV) Ions from a High-Concentration Oxalic Acid Solution by a Macroporous Silica-Based <i>N,N,N′,N′</i>-Tetraoctyl-3-Oxapentane-1,5-Diamide Polymeric Adsorbent (2004)

To separate Mo(VI) and Zr(IV) ions from a 0.5 M oxalic acid solution and from each other, a novel macroporous silica-based N,N,N′,N′-tetraoctyl-3-oxapentane-1,5-diamide (TODGA) chelating polymeric adsorbent (TODGA/SiO 2 -P) was synthesized by introducing the TODGA molecule into ca. 50-μm diameter SiO 2 -P particles by impregnation. The adsorption of Mo(VI) and Zr(IV) ions onto TODGA/SiO 2 -P was investigated by examining the influence of nitric acid and oxalic acid concentrations. It was found that the adsorption was strongly affected by increasing the HNO 3 concentration from 0.5 M to 9.0 M either with or without the addition of 0.5 M H 2 C 2 O 4 . In the absence of 0.5 M H 2 C 2 O 4 , the distribution coefficients (K d ) of both Mo(VI) and Zr(IV) ions decreased with an increase in the HNO 3 concentration from 0.5 M to 3.0 M, increased slowly when the concentration of HNO 3 was increased further from 3.0 M to 6.0 M, and then increased gradually above 6.0 M concentration. Furthermore, the adsorption of Zn(IV) ions onto TODGA/SiO 2 -P was considerably greater than that of Mo(VI) ions. In the presence of 0.5 M H 2 C 2 O 4 , Zr(IV) ions showed no adsorption at HNO 3 concentrations below 4.0 M, being partly adsorbed in the presence of 4.0–6.0 M HNO 3 and fully adsorbed when the HNO 3 concentration was greater than 6.0 M. In contrast, Mo(VI) ions showed a much lower adsorption at HNO 3 concentrations below 2.0 M and no adsorption above this acid level. In the presence of HNO 3 at a concentration above 6.0 M and containing 0.5 M H 2 C 2 O 4 , the adsorption of Zr(IV) ions overlapped that from a similar HNO 3 solution which did not include 0.5 M H 2 C 2 O 4 . This was attributed to complete protonation of the C 2 O 2− 2 anion. On the basis of batch experiments, Mo(VI) and Zr(IV) ions were separated from a 6.5 M HNO 3 solution containing 0.5 M H 2 C 2 O 4 by means of a column packed with TODGA/SiO 2 -P at 50°C. The separate components, Mo(VI) and Zr(IV), could be effectively separated from each other by eluting with some selected eluants. The recovery percentage was 100.5% for Mo(VI) ions and 96.8% for Zr(IV) ions.
Graphical Analysis of Reversible Radioligand Binding from Time—Activity Measurements Applied to [<i>N</i>-<sup>11</sup>C-Methyl]-(−)-Cocaine PET Studies in Human Subjects (1990)

A graphical method of analysis applicable to ligands that bind reversibly to receptors or enzymes requiring the simultaneous measurement of plasma and tissue radioactivities for multiple times after the injection of a radiolabeled tracer is presented. It is shown that there is a time t after which a plot of integral of t0ROI(t')dt'/ROI(t) versus integral of t0Cp(t')dt'/ROI(t) (where ROI and Cp are functions of time describing the variation of tissue radioactivity and plasma radioactivity, respectively) is linear with a slope that corresponds to the steady-state space of the ligand plus the plasma volume,.Vp. For a two-compartment model, the slope is given by lambda + Vp, where lambda is the partition coefficient and the intercept is -1/[kappa 2(1 + Vp/lambda)]. For a three-compartment model, the slope is lambda(1 + Bmax/Kd) + Vp and the intercept is -[1 + Bmax/Kd)/k2 + [koff(1 + Kd/Bmax)]-1) [1 + Vp/lambda(1 + Bmax/Kd)]-1 (where Bmax represents the concentration of ligand binding sites and Kd the equilibrium dissociation constant of the ligand-binding site complex, koff (k4) the ligand-binding site dissociation constant, and k2 is the transfer constant from tissue to plasma). This graphical method provides the ratio Bmax/Kd from the slope for comparison with in vitro measures of the same parameter. It also provides an easy, rapid method for comparison of the reproducibility of repeated measures in a single subject, for longitudinal or drug intervention protocols, or for comparing experimental results between subjects. Although the linearity of this plot holds when ROI/Cp is constant, it can be shown that, for many systems, linearity is effectively reached some time before this. This analysis has been applied to data from [N-methyl-11C]-(-)-cocaine ([11C]cocaine) studies in normal human volunteers and the results are compared to the standard nonlinear least-squares analysis. The calculated value of Bmax/Kd for the high-affinity binding site for cocaine is 0.62 +/- 0.20, in agreement with literature values.
Stereo and 3‐<scp>D</scp>Display Technologies (2002)

Abstract Recently, there have been rapid advancements in 3‐D techniques and technologies. Hardware has improved and become considerably cheaper, making real‐time and interactive 3‐D available to the hobbyist, as well as to the researcher. There have been major studies in areas such as molecular modeling, photogrammetry, flight simulation, CAD, visualization of multidimensional data, medical imaging, teleoperations such as remote vehicle piloting and remote surgery, and stereolithography. In computer graphics, the improvements in speed, resolution, and economy make interactive stereo an important capability. Old techniques have been improved, and new ones have been developed. True 3‐D is rapidly becoming an important part of computer graphics, visualization, virtual‐reality systems, and computer gaming. Numerous 3‐D systems are granted patents each year, but very few systems move beyond the prototype stage and become commercially viable. Here, the salient 3‐D systems are treated. First, the major depth cues that one uses to determine depth relationships among objects in a scene are described. The history of 3‐D displays and an overview of technologies follow. Details are given on the viewing devices required. Large formats, such as IMAX are discused. Autostereoscopic displays with us viewing devices are discussed.
Refractometers and tunable components based on side-polished fibers with multimode overlay waveguides: role of the superstrate (1996)

A normal mode analysis is applied to the investigation of the role of the superstrate in terms of its refractive index and thickness in estimating the performance of fiber refractometers and the tunability of fiber components based on evanescent coupling of a side-polished fiber to a multimode overlay waveguide. The model is shown to yield a complete transfer function and information about all the major characteristics of such in-line fiber devices. The results closely match the trends of the reported experiments.
Field-Induced Metamagnetic Transition in the Fe<sup>III</sup>−Mn<sup>III</sup> Bimetallic Chain Built by a New Cyanide-Bearing Fe<sup>III</sup> Precursor (2007)

The use of a new precursor, mer-[Fe(mpzcq)(CN)3]- (1), produced a dimeric molecule, [Fe(mpzcq)(CN)3][Mn(salen)(H2O)] x H2O (2), and a one-dimensional zigzag chain, [Fe(mpzcq)(CN)3][Mn(salcy)] x MeOH x MeCN (3). Antiferromagnetic couplings are operating between magnetic centers through CN ligands, and a field-induced metamagnetic transition is observed in 3.