gebhart/patrepeval huggingface dataset:
A Harpin Binding Site in Tobacco Plasma Membranes Mediates Activation of the Pathogenesis-Related Gene <i>HIN1</i> Independent of Extracellular Calcium but Dependent on Mitogen-Activated Protein Kinase Activity (2001)

Harpin from the bean halo-blight pathogen Pseudomonas syringae pv phaseolicola (harpin(Psph)) elicits the hypersensitive response and the accumulation of pathogenesis-related gene transcripts in the nonhost plant tobacco. Here, we report the characterization of a nonproteinaceous binding site for harpin(Psph) in tobacco plasma membranes, which is assumed to mediate the activation of plant defense responses in a receptor-like manner. Binding of 125I-harpin(Psph) to tobacco microsomal membranes (dissociation constant = 425 nM) and protoplasts (dissociation constant = 380 nM) was specific, reversible, and saturable. A close correlation was found between the abilities of harpin(Psph) fragments to elicit the transcript accumulation of the pathogenesis-related tobacco gene HIN1 and to compete for binding of 125I-harpin(Psph) to its binding site. Another elicitor of the hypersensitive response and HIN1 induction in tobacco, the Phytophthora megasperma-derived beta-elicitin beta-megaspermin, failed to bind to the putative harpin(Psph) receptor. In contrast to activation by beta-megaspermin, harpin(Psph)-induced activation of the 48-kD salicylic acid-responsive mitogen-activated protein kinase (MAPK) and HIN1 transcript accumulation were independent of extracellular calcium. Moreover, use of the MAPK kinase inhibitor U0126 revealed that MAPK activity was essential for pathogenesis-related gene expression in harpin(Psph)-treated tobacco cells. Thus, a receptor-mediated MAPK-dependent signaling pathway may mediate the activation of plant defense responses induced by harpin(Psph).
Near-diffraction-limited single-lobe emission from a high-power diode-laser array coupled to a photorefractive self-pumped phase-conjugate mirror (1991)

Details of a 20-stripe, 1-W diode-laser array coupled to a photorefractive self-pumped phase-conjugate mirror in a self-injection-locking geometry are discussed. Single-lobe emission is observed, with 75% of the array output in an ~2.2 times diffraction-limited beam, for array output powers of as much as 450 mW.
Effect of Fluticasone Furoate and Vilanterol on Exacerbations of Chronic Obstructive Pulmonary Disease in Patients with Moderate Airflow Obstruction (2016)

Patients with moderate chronic airflow obstruction experienced a reduction in exacerbations with FF/VI compared with placebo, irrespective of a history of exacerbations or baseline FEV<sub>1</sub>. Clinical trial registered with www.clinicaltrials.gov (NCT 01313676; GSK Study number 113782).
Security at the network edge: a distributed firewall architecture (2002)

We introduce Network Edge Security, a new distributed firewall architecture designed to counter the insider threat, which is undeterred by existing firewall implementations. Network Edge Security also addresses the security challenges of emerging technology trends like mobile computing, business to business computing and virtual private networks (VPNs). We describe the architecture and the rationale for its design, then we illustrate its application.
FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells (2008)

Autophagy is a membrane-mediated intracellular degradation system. The serine/threonine kinase Atg1 plays an essential role in autophagosome formation. However, the role of the mammalian Atg1 homologues UNC-51-like kinase (ULK) 1 and 2 are not yet well understood. We found that murine ULK1 and 2 localized to autophagic isolation membrane under starvation conditions. Kinase-dead alleles of ULK1 and 2 exerted a dominant-negative effect on autophagosome formation, suggesting that ULK kinase activity is important for autophagy. We next screened for ULK binding proteins and identified the focal adhesion kinase family interacting protein of 200 kD (FIP200), which regulates diverse cellular functions such as cell size, proliferation, and migration. We found that FIP200 was redistributed from the cytoplasm to the isolation membrane under starvation conditions. In FIP200-deficient cells, autophagy induction by various treatments was abolished, and both stability and phosphorylation of ULK1 were impaired. These results suggest that FIP200 is a novel mammalian autophagy factor that functions together with ULKs.
Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement (1993)

Shigella flexneri uses elements of the host cell cytoskeleton to move within cells and from cell to cell. IcsA, an S. flexneri protein involved in this movement, was purified and studied in vitro. IcsA bound the radiolabelled ATP analog 3'(2')-O-(4-benzoyl)benzoyl-ATP and hydrolyzed ATP. In addition, the surface localization of IcsA on both extracellular and intracellular shigellae was unipolar. Further, in HeLa cells infected with shigellae, IcsA antiserum labelled the actin tail throughout its length, thereby suggesting that IcsA interacts with elements within the tail. Localization of IcsA within the tail at a distance from the bacterium would require its secretion; we demonstrate here that in vitro IcsA is secreted into the culture supernatant in a cleaved form.
Parametric generation of tunable infrared radiation in ZnGeP_2 and GaSe pumped at 3 μm (1993)

Traveling-wave parametric generators with angular tuning with the use of ZnGeP2 and GaSe crystals pumped by 100-ps pulses from an actively mode-locked Er laser (λ ≈ 3 μm) are reported. The continuous-tuning range achieved was 4–10 μm (ZnGeP2; length, 12 mm) and 3.5–18 μm (GaSe; length, 12 mm) in a type-I interaction, with a quantum conversion efficiency of a few percent. In the case of ZnGeP2 (length, 42 mm) and type-II phase matching, the pump threshold was 0.35 GW/cm2 and the quantum efficiency achieved was 17.6%, corresponding to an output peak power of 3 MW near 5–6 μm.
Hypothalamic hormones. 27. Synthesis of di- and tripeptides and assay in vivo for activity in the thyrotropin releasing hormone and the luteinizing releasing hormone systems (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHypothalamic hormones. 27. Synthesis of di- and tripeptides and assay in vivo for activity in the thyrotropin releasing hormone and the luteinizing releasing hormone systemsHans Sievertsson, Jaw-Kang Chang, Karl Folkers, and Cyril Y. BowersCite this: J. Med. Chem. 1972, 15, 1, 8–11Publication Date (Print):January 1, 1972Publication History Published online1 May 2002Published inissue 1 January 1972https://pubs.acs.org/doi/10.1021/jm00271a003https://doi.org/10.1021/jm00271a003research-articleACS PublicationsRequest reuse permissionsArticle Views49Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Hydrolysis of a metal-coordinated imine by a proton-containing transition state (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrolysis of a metal-coordinated imine by a proton-containing transition stateTamera K. Cole and R. G. LinckCite this: Inorg. Chem. 1988, 27, 8, 1498–1502Publication Date (Print):April 1, 1988Publication History Published online1 May 2002Published inissue 1 April 1988https://doi.org/10.1021/ic00281a037Request reuse permissionsArticle Views99Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (731 KB) Get e-Alertsclose Get e-Alerts
A TWT grid modulator for MTI radar transmitters (2002)

The design of HV (high-voltage) pulse circuitry using series connected HV MOSFETs is traced from the development of the basic switching module through integration into a TWT (traveling wave tube) modulator. The modulator deck floats at the -45.5 kV TWT cathode potential and supplies filament power and control grid pulses directly to dual TWTs. Prime power is 115 VAC, 70 Hz, single phase. Control signals, start and stop pulses and fault signals are transmitted via a multichannel fiber optic link. RF stability measurements made on the transmitter have confirmed the adequacy of the modulator design.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Percutaneous caval stent valve implantation: investigation of an interventional approach for treatment of tricuspid regurgitation (2009)

In high-grade tricuspid insufficiency, percutaneous implantation of valved stents in the central venous position reduces venous regurgitation and improves haemodynamics in the animal experiment. Implantation of one or two valves in central venous position is technically feasible. Functional replacement of the insufficient tricuspid valve leads to an increase in CO. This technique expands the potential therapeutic options for patients with relevant tricuspid valve regurgitation having a high risk for open heart surgery.
STIMULATION IN DIFFERENT PHASES OF THE CARDIAC CYCLE AND REACTION TIME (1970)

ABSTRACT Several studies have suggested that reaction time (RT) may be related to variations in blood pressure which occur with each heart beat. This was tested in a series of four studies in which possible effects of the preparatory interval (PI) were controlled. Stimuli were presented at 0, 200, 400, and 600 msec following the R wave, and during the ascending slope of the R, T, and P waves of the cardiac cycle. No relationship was found in any of the four studies between RT and the phase within the cardiac cycle when the stimulus occurred.
Collaborative Gatekeeping Between Site Supervisors and Mental Health Counseling Faculty (2018)

This study examined collaborative gatekeeping practices of CACREP-accredited (2001 and 2009 standards) mental health counseling programs and fieldwork site supervisors. A total of 28 programs participated in the study exploring site supervisor collaboration with fieldwork faculty when a problematic trainee is terminated, dismissed, or fired from a fieldwork site. Results indicate that a lack of clear policies for managing problem fieldwork trainees may leave counselor educators uncertain about appropriate follow-up actions. Recommendations for future studies include replicating this study using a larger sample size, studying this topic and population using qualitative methods, and surveying the perspective of site supervisors.
Biological applications of infrared spectroscopy (1997)

Instrumentation. Sampling Methods. Spectrum Interpretation. Quantitative Analysis. Applications. Self-Assessment Questions and Responses. Units of Measurement. Index.
Studies of the mitogenic effect of serotonin in rat renal mesangial cells (1989)

A vasoactive inflammatory amine, serotonin, stimulates DNA synthesis in rat glomerular mesangial cells in a dose-dependent manner and acts synergistically with either insulin or epidermal growth factor (EGF). The combined effects of 10(-6) M serotonin and these peptide hormones are nearly equal to those induced by 10% fetal bovine serum. Serotonin stimulates the turnover of polyphosphoinositols resulting in a transient rise in intracellular free Ca2+ concentration, as measured either with the photoprotein aequorin, or with fura-2. This is accompanied by a transient increase in 45Ca2+ efflux from prelabeled cells. Serotonin also induces a prompt and sustained threefold increase in Ca2+ influx rate across the plasma membrane and a rapid and sustained twofold increase in cellular 1,2-diacylglycerol content. In addition, there is an increase in the extent of phosphorylation of an acidic 80-kDa protein, a putative substrate for protein kinase C. Activators of protein kinase C (including phorbol 12-myristate 13-acetate or 1,2-dioctanoylglycerol) mimic the mitogenic effect of serotonin. The effect of serotonin on cell proliferation is partially inhibited in a reversible manner by LiCl. Treatment of mesangial cells with insulin plus EGF for 60 min leads to a small but consistent increase in the content of inositol phosphates and 1,2-diacylglycerol. Their effects are additive to those of serotonin. Moreover, insulin and EGF significantly stimulate the phosphorylation of the 80-kDa protein, and potentiate the serotonin-induced phosphorylation of this protein.(ABSTRACT TRUNCATED AT 250 WORDS)
Global White Matter Analysis of Diffusion Tensor Images Is Predictive of Injury Severity in Traumatic Brain Injury (2007)

Conventional clinical neuroimaging is insensitive to axonal injury in traumatic brain injury (TBI). Immunocytochemical staining reveals changes to axonal morphology within hours, suggesting potential for diffusion-weighted magnetic resonance (MR) in early diagnosis and management of TBI. Diffusion tensor imaging (DTI) characterizes the three-dimensional (3D) distribution of water diffusion, which is highly anisotropic in white matter fibers owing to axonal length. Recently, DTI has been used to investigate traumatic axonal injury (TAI), emphasizing regional analysis in more severe TBI. In the current study, we hypothesized that a global white matter (WM) analysis of DTI data would be sensitive to TAI across a spectrum of TBI severity and injury to scan interval. To investigate this, we compared WM-only histograms of a scalar, fractional anisotropy (FA), between 20 heterogeneous TBI patients recruited from Detroit Medical Center, including six mild TBI (GCS 13-15), and 14 healthy age-matched controls. FA histogram parameters were correlated with admission GCS and posttraumatic amnesia (PTA). In all cases, including mild TBI, patients' FA histograms were globally decreased compared with control histograms. The shape of the TBI histograms also differed from controls, being more peaked and skewed. The mean FA, kurtosis and skewness were highly correlated suggesting a common mechanism. FA histogram properties also correlated with injury severity indexed by GCS and PTA, with mean FA being the best predictor and duration of PTA (r = 0.64) being superior to GCS (r = 0.47). Therefore, in this heterogeneous sample, the FA mean accounted for 40% of the variance in PTA. Increased diffusion in the short axis dimension, likely reflecting dysmyelination and swelling of axons, accounted for most of the FA decrease. FA is globally deceased in WM, including mild TBI, possibly reflecting widespread involvement. FA changes appear to be correlated with injury severity suggesting a role in early diagnosis and prognosis of TBI.
Super spectral resolution beyond pixel Nyquist limits on multi-channel spectrometer (2016)

Enhancement of the resolution of well-distributed multi-channel spectrometers beyond the pixel Nyquist limits while maintaining their inherent advantages such as high-speed measurement, compactness, and robustness will represent a critical step toward real-time monitoring of dynamic events. Here, we report the first super spectral resolution measurement beyond pixel Nyquist limits which was made possible by employing the Moiré effect in a commercially available Czerny-Turner type spectroscope of 4.63nm-spectral resolution. The experimental results show that the spectral resolution can be enhanced up to 0.31 nm by a factor of more than 10 with exceeding the 50-μm pixel Nyquist limits.
Two-Dimensional Resistivity Inversion with Smoothness Constraint (1990)

In two-dimensional (2D) resistivity inversion, since more flexible model requires additional parameters to be estimated, a nonlinear least-squares (LS) solution usually becomes unstable. The use of smoothness constraint in the nonlinear LS technique is effective to stabilize the nonlinear LS solution. In this paper 2D resistivity inversion technique with smoothness constraint is developed to improve the resolution of 2D model. The usefulness of this inversion method is proved by interpreting four groups of field data. The inversion method is convenient especially in construction of 2D block model for geological situation with little field informations.
Impinging Stream Reactors: Fundamentals and Applications (1995)

"Impinging Stream Reactors: Fundamentals and Applications." Drying Technology, 13(1-2), pp. 503–504
A cellular platform for the development of synthetic living machines (2021)

Robot swarms have, to date, been constructed from artificial materials. Motile biological constructs have been created from muscle cells grown on precisely shaped scaffolds. However, the exploitation of emergent self-organization and functional plasticity into a self-directed living machine has remained a major challenge. We report here a method for generation of in vitro biological robots from frog (<i>Xenopus laevis</i>) cells. These xenobots exhibit coordinated locomotion via cilia present on their surface. These cilia arise through normal tissue patterning and do not require complicated construction methods or genomic editing, making production amenable to high-throughput projects. The biological robots arise by cellular self-organization and do not require scaffolds or microprinting; the amphibian cells are highly amenable to surgical, genetic, chemical, and optical stimulation during the self-assembly process. We show that the xenobots can navigate aqueous environments in diverse ways, heal after damage, and show emergent group behaviors. We constructed a computational model to predict useful collective behaviors that can be elicited from a xenobot swarm. In addition, we provide proof of principle for a writable molecular memory using a photoconvertible protein that can record exposure to a specific wavelength of light. Together, these results introduce a platform that can be used to study many aspects of self-assembly, swarm behavior, and synthetic bioengineering, as well as provide versatile, soft-body living machines for numerous practical applications in biomedicine and the environment.
Tunable transmission and bistability in left-handed band-gap structures (2004)

We study the defect-induced nonlinear transmission of a periodic structure created by alternating slabs of two materials with positive and negative refractive index. We demonstrate bistable switching and tunable nonlinear transmission in an unconventional band gap that corresponds to the vanishing average refractive index, and compare the observed effects for two different types of band gaps.
[Breeding of monofluoroacetate-resistant strains of Actinobacillus succinogenes and the mechanism based on metabolic flux analysis]. (2008)

Succinic acid has received a great deal of attention as an important green chemical stock for the manufacture of synthetic resins, biodegradable polymers and chemical intermediates. In this paper, the breeding mechanism of Actinobacillus succinogenes based on metabolic flux analysis was demonstrated to improve the yield of succinic acid by fermentation. After the NTG treatment, mutants from A. succinogenes CGMCC 1593 which were able to grow in medium containing concentrations of about 50-100 mmol/L of sodium monofluoroacetate were obtained. Among them, a mutant SF-9 was selected for producing more succinic acid and less acetic acid. When fermentations were conducted in a 5 L bioreactors, the final succinic acid concentration of SF-9 (34.8 g/L) increased 23.4%, and the mass ratio of succinic acid/acetic acid increased from 3.3 to 9 compared with those of the parent strain. Based on the metabolic flux analysis of A. succinogenes, PEP was found to be a key node which has an important effect on the production of succinic acid, and the flux ratio of by-productions (acetic, formic, lactic acid) was influenced by PYR node. Compared with the parent strain, the flux to succinic acid of mutant (A. succinogenes SF-9) was significantly increased, while the flux to by-productions had an obvious decline. Therefore, PEP and PYR are not rigid nodes in the metabolic regulation of A. succinogenes.
Optical Flow Estimation for Flame Detection in Videos (2013)

Computational vision-based flame detection has drawn significant attention in the past decade with camera surveillance systems becoming ubiquitous. Whereas many discriminating features, such as color, shape, texture, etc., have been employed in the literature, this paper proposes a set of motion features based on motion estimators. The key idea consists of exploiting the difference between the turbulent, fast, fire motion, and the structured, rigid motion of other objects. Since classical optical flow methods do not model the characteristics of fire motion (e.g., non-smoothness of motion, non-constancy of intensity), two optical flow methods are specifically designed for the fire detection task: optimal mass transport models fire with dynamic texture, while a data-driven optical flow scheme models saturated flames. Then, characteristic features related to the flow magnitudes and directions are computed from the flow fields to discriminate between fire and non-fire motion. The proposed features are tested on a large video database to demonstrate their practical usefulness. Moreover, a novel evaluation method is proposed by fire simulations that allow for a controlled environment to analyze parameter influences, such as flame saturation, spatial resolution, frame rate, and random noise.
Four-point SC-cut crystal unit design calculations for reduced acceleration sensitivity (2003)

Efforts have been directed toward development of a readily manufacturable four point mount which minimizes acceleration sensitivity for 10 MHz, 3rd, SC-cut crystal units. Design criteria have been established for the mounts to improve crystal unit precision and reduce acceleration sensitivity. A four point mount, called the horizontal strip mount, satisfies the design criteria. Lower acceleration sensitivities are predicted for the horizontal strip mount design, and optimum mode locations are in closer proximity to the resonator center over all mount orientations. Calculations have been performed which characterize variations in acceleration sensitivity with changes in critical dimensions for the horizontal strip mount. Optimum mount dimensions which minimize acceleration sensitivity have been calculated.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>