gebhart/patrepeval huggingface dataset:
60-kDa Ro protein autoepitopes identified using recombinant polypeptides. (1994)

The human Ro ribonucleoprotein is a clinically important yet poorly understood autoantigen. The contribution of Ro autoantibodies to pathogenesis of autoimmune disease remains unclear, as do the stimuli that initiate and maintain the response. Recent evidence suggests that patient anti-Ro responses target individual proteins, including a 60- and a 52-kDa species, within the complex in a disease-specific manner. However, Ro antisera retain considerable heterogeneity in their recognition of both continuous and discontinuous epitopes on the protein components. Previous characterization of Ro autoepitopes has primarily involved solid phase assays, which are of limited value in identifying discontinuous epitopes. To address the heterogeneity of Ro and the issue of discontinuous autoepitopes, we have generated 10 overlapping recombinant polypeptides of the human 60-kDa Ro protein and compared their reactivities using a soluble immunoprecipitation assay. Seven different epitopes, both continuous and discontinuous, were distinguished and seven distinct patterns of reactivity were discerned among the sera from 12 patients. These patterns of reactivity showed no relationship to clinical diagnosis but did correlate with the titer of Abs against recombinant 60-kDa Ro and with the concomitant presence of Abs directed against recombinant 52-kDa Ro protein. Sera that immunoprecipitated only the full-length 60-kDa protein had low or undetectable anti-60 kDa titers by ELISA and immunoblot using recombinant Ag, demonstrating a predominant recognition of discontinuous epitopes. These data indicate that autoantibody responses to the 60-kDa Ro Ag can preferentially target discontinuous epitopes and that the ability to recognize continuous epitopes is accompanied by the appearance of 52-kDa Ro autoantibodies.
Potential Multifunctional Inhibitors of HIV-1 Reverse Transcriptase. Novel [AZT]-[TSAO-T] and [d4T]-[TSAO-T] Heterodimers Modified in the Linker and in the Dideoxynucleoside Region (1999)

In an attempt to combine the anti-HIV-inhibitory capacity of nucleoside reverse transcriptase (RT) inhibitors (NRTI) and non-nucleoside RT inhibitors (NNRTI), several heterodimer analogues of the previously reported [AZT]-(CH(2))(3)-[TSAO-T] prototype have been prepared. In these novel series, other NRTIs, an expanded range of linkers with different conformational freedom and other attachment sites for these linkers on the base part of the NRTI analogue have been explored. Moreover, in order to circumvent the dependence of the NRTI moiety of the heterodimer on activation by cellular nucleoside kinases, novel heterodimers in which the NRTI is bearing a masked monophosphate group at the 5'-position are described. Among the novel heterodimers, several derivatives show a potent anti-HIV-1 activity, which proved comparable, or even superior, to that of the AZT heterodimer prototype. The nature of the NRTI was important for the eventual anti-HIV-1 activity. In particular, the d4T heterodimer derivative containing a propyl linker between the N-3 positions of the base of TSAO-T and d4T was approximately 5- to 10-fold more inhibitory to HIV-1 than the corresponding AZT heterodimer prototype.
Retinal Hemorrhages Associated With Ocular Decompression After Glaucoma Surgery (1996)

Two cases of retinal hemorrhages in glaucomatous eyes associated with ocular decompression following glaucoma filtration surgery are presented. Both patients were young, healthy men with markedly elevated preoperative intraocular pressures (IOPs). The hemorrhages were blot shaped and scattered throughout the macula (including the fovea) in each eye that underwent surgery. One eye had hemorrhages with distinct white centers. Each eye that underwent surgery experienced a permanent decrease in visual acuity after resolution of the hemorrhages. A gradual decrease of IOP preoperatively and intraoperatively is recommended in order to avoid this complication.
Strongly luminescent monolayered MoS2 prepared by effective ultrasound exfoliation (2013)

Intense ultrasound in a pressurized batch reactor was used for preparation of monolayered MoS2 nanosheets from natural mineral molybdenite. Exfoliation of bulk MoS2 using ultrasound is an attractive route to large-scale preparation of monolayered crystals. To evaluate the quality of delamination, methods like X-ray diffraction, Raman spectroscopy and microscopic techniques (TEM and AFM) were employed. From single- or few-layered products obtained from intense sonication, MoS2 quantum dots (MoSQDs) were prepared by a one-pot reaction by refluxing exfoliated nanosheets of MoS2 in ethylene glycol under atmospheric pressure. The synthesised MoSQDs were characterised by photoluminescence spectroscopy and laser-scattering particle size analysis. Our easy preparation leads to very strongly green luminescing quantum dots.
Ubiquitination of STING at lysine 224 controls IRF3 activation (2017)

The E3 ligase MUL1 regulates STING-dependent innate immune responses to cytosolic DNA.
Use of synthetic peptides to probe lymphocyte- high endothelial cell interactions. Lymphocytes recognize a ligand on the endothelial surface which contains the CS1 adhesion motif (1990)

The extravasation of recirculating lymphocytes into lymph nodes, which is crucial for immune system function, occurs constitutively from specialized post-capillary venules in the lymph node paracortex. The migration of lymphocytes between the structurally distinct high endothelial cells which line these blood vessels is a rapid process involving highly specific cellular recognition events. Although a number of lymphocyte surface molecules have been identified that mediate adhesion to high endothelial cells (the first step in extravasation), the equally important endothelial molecules which serve as their ligands are still poorly understood. By using a novel in vitro model of lymphocyte-high endothelial cell recognition, together with a series of anti-adhesive synthetic peptides, we have assessed the role of the adhesive glycoprotein fibronectin in this process. We report here that CS1, a 25-mer sequence representing the major cell recognition site within the alternatively spliced type III connecting segment of fibronectin, supports the adhesion of rat lymphocytes and that it is a specific inhibitor of lymphocyte adhesion to the surface of high endothelial cells. These results identify a novel ligand on high endothelial cells containing the CS1 adhesion motif (possibly a cell-surface form of fibronectin) which mediates the adhesion of lymphocytes.
Topical use of adrenaline in different concentrations for endoscopic sinus surgery (2009)

We favor the use of topical adrenalin 1:2,000 due to a clear superiority in hemostasis. Further investigation is needed to corroborate our findings.
A formal approach to analyzing the browsing semantics of hypertext (1994)

Browsing through a hyperdocument often leads to orientation problems: &quot;you don&apos;t know where you are, you are lost in hyperspace&quot;. Although some of these problems may be diminished by specific browsing tools, such as fish-eye views and history mechanisms, most problems are embedded in the structure of the hyperdocument. In order to detect and analyze potential problems in the use of a hyperdocument, we reduce different types of browsing actions to a unified and well know formalism : the Petri net. In this paper we use the object-oriented model from [3] to describe hyperdocuments. This model, unlike other reference models [5, 6, 9], describes objects using a number of different description layers: these layers are not fixed by the model, and none of them are excluded from the model. In order to use Petri nets to describe browsing, the operations dealing with the hierarchical structure of objects (composites, towers and cities) are translated into operations that are equivalent to the sim...
Sampling and determination of gas-phase hydrogen peroxide following removal of ozone by gas-phase reaction with nitric oxide (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSampling and determination of gas-phase hydrogen peroxide following removal of ozone by gas-phase reaction with nitric oxideRoger L. Tanner, George Y. Markovits, Eugene M. Ferreri, and Thomas J. KellyCite this: Anal. Chem. 1986, 58, 8, 1857–1865Publication Date (Print):July 1, 1986Publication History Published online1 May 2002Published inissue 1 July 1986https://pubs.acs.org/doi/10.1021/ac00121a056https://doi.org/10.1021/ac00121a056research-articleACS PublicationsRequest reuse permissionsArticle Views238Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Integrated circuits metering for piracy protection and digital rights management (2011)

This paper presents an overview of hardware and Integrated Circuits (IC) metering methods. IC metering or hardware metering refers to tools, methodologies, and protocols that enable post-fabrication tracking of the ICs. Metering enables prevention and detection of overbuilt and counterfeit ICs in the dominant semiconductor contract-foundry model. Post-silicon identification and tagging of the individual ICs fabricated by the same mask is a precursor for metering: In passive metering, the ICs are specifically identified, either in terms of their functionality, or by other forms of unique identification. The identified ICs may be matched against their record in a pre-formed database that could reveal unregistered ICs or overbuilt ICs (in case of collisions). In active metering, not only the ICs are uniquely identified, but also parts of the chip's functionality can be only accessed, locked (disabled), or unlocked (enabled) by the designer and/or IP rights owners using a high level knowledge of the design not transferred to the foundry. We provide a systematic overview of the field, along with a taxonomy of available methods.
Piezoelectric Poly(vinylidene fluoride) in Small-Bore, Thick-Walled Tubular Form (1984)

The preparation of piezoelectric poly(vinylidene fluoride) (PVDF) from pellets usually involves several batch processes. These are time consuming and inevitably lead to wastage, lack of uniformity in large quantities, and extra costs. In particular, the manufacture of thick film PVDF (> 100 μm) has required expensive preparative equipment and very high poling voltages. This paper describes a low cost continuous process in which pellets are directly converted into small-bore, thick-walled piezoelectric tube. A small commercial extruder was used to produce PVDF tube (crystal form II), and in-line heating, stretching and corona poling between two haul-offs produced a poled tube (crystal form I) of true draw ratio ~ 4:1. Production rates of metres per minute were obtained using conveniently low poling fields. For example, tubing of o.d. 1.4 mm and wall thickness 234 μm showed piezoelectric coefficients d31 19 pC/N and dh 14 pC/N, and a pyroelectric coefficient λ 29 μC/m2K, with good ageing properties up to 120°C. The tube can be produced with or without a tightly fitting wire core. Applications are suggested as an underwater sonar transducer and as a pyroelectric detector for absorption loss measurements on low-loss optical fibre.
A simple diagnostic milk medium for <i>Pseudomonas aeruginosa</i> (1970)

An agar medium containing 10% defatted milk has been tested as a diagnostic medium for Pseudomonas aeruginosa and, in particular, for differentiating between P. aeruginosa and P. fluorescens. Only P. aeruginosa colonies gave clear zones due to hydrolysis of casein together with diffused green pigment. Results on milk agar correlated well with the pattern of results from a variety of conventional tests used to identify this organism. Pigment production of P. aeruginosa on milk agar was better than on special media commonly used to enhance this characteristic. Routine diagnosis of P. aeruginosa is recommended by streaking on a solid medium containing 10% defatted milk granules, 25% nutrient broth, and 2% agar, and examining for clear zones and pigment after 24 hours' incubation.
Reactively sputtered nitrides and carbides of titanium, zirconium, and hafnium (1986)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation William D. Sproul; Reactively sputtered nitrides and carbides of titanium, zirconium, and hafnium. J. Vac. Sci. Technol. A 1 November 1986; 4 (6): 2874–2878. https://doi.org/10.1116/1.573651 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAVS: Science & Technology of Materials Interfaces and ProcessingJournal of Vacuum Science & Technology A Search Advanced Search |Citation Search
XVIII. <i>On the passage of electric waves through tubes, or the vibrations of dielectric cylinders</i> (1897)

(1897). XVIII. On the passage of electric waves through tubes, or the vibrations of dielectric cylinders . The London, Edinburgh, and Dublin Philosophical Magazine and Journal of Science: Vol. 43, No. 261, pp. 125-132.
Cytosolic Ca<sup>2+</sup> triggers early afterdepolarizations and torsade de pointes in rabbit hearts with type 2 long QT syndrome (2002)

The role of intracellular Ca2+ (Ca2+i) in triggering early afterdepolarizations (EADs), the origins of EADs and the mechanisms underlying Torsade de Pointes (TdP) were investigated in a model of long QT syndrome (Type 2). Perfused rabbit hearts were stained with RH327 and Rhod-2/AM to simultaneously map membrane potential (V(m)) and Ca2+i with two photodiode arrays. The I(Kr) blocker E4031 (0.5 microM) together with 50 % reduction of [K+]o and [Mg2+]o elicited long action potentials (APs), V(m) oscillations on AP plateaux (EADs) then ventricular tachycardia (VT). Cryoablation of both ventricular chambers eliminated Purkinje fibres as sources of EADs. E4031 prolonged APs (0.28 to 2.3 s), reversed repolarization sequences (baseapex) and enhanced repolarization gradients (30 to 230 ms, n = 12) indicating a heterogeneous distribution of I(Kr). At low [K+]o and [Mg2+]o, E4031 elicited spontaneous Ca2+iand V(m) spikes or EADs (3.5 +/- 1.9 Hz) during the AP plateau (n = 6). EADs fired 'out-of-phase' from several sites, propagated, collided then evolved to TdP. Phase maps (Ca2+ivs. V(m)) had counterclockwise trajectories shaped like a 'boomerang' during an AP and like ellipses during EADs, with V(m) preceding Ca2+iby 9.2 +/- 1.4 (n = 6) and 7.2 +/- 0.6 ms (n = 5/6), respectively. After cryoablation, EADs from surviving epicardium (~1 mm) fired at the same frequency (3.4 +/- 0.35 Hz, n = 6) as controls. At the origins of EADs, Ca2+ipreceded V(m) and phase maps traced clockwise ellipses. Away from EAD origins, V(m) coincided with or preceded Ca2+i. In conclusion, overload elicits EADs originating from either ventricular or Purkinje fibres and 'out-of-phase' EAD activity from multiple sites generates TdP, evident in pseudo-ECGs.
Estimation of Nucleotide Diversity, Disequilibrium Coefficients, and Mutation Rates from High-Coverage Genome-Sequencing Projects (2008)

Recent advances in sequencing strategies have made it feasible to rapidly obtain high-coverage genomic profiles of single individuals, and soon it will be economically feasible to do so with hundreds to thousands of individuals per population. While offering unprecedented power for the acquisition of population-genetic parameters, these new methods also introduce a number of challenges, most notably the need to account for the binomial sampling of parental alleles at individual nucleotide sites and to eliminate bias from various sources of sequence errors. To minimize the effects of both problems, methods are developed for generating nearly unbiased and minimum-sampling-variance estimates of a number of key parameters, including the average nucleotide heterozygosity and its variance among sites, the pattern of decomposition of linkage disequilibrium with physical distance, and the rate and molecular spectrum of spontaneously arising mutations. These methods provide a general platform for the efficient utilization of data from population-genomic surveys, while also providing guidance for the optimal design of such studies.
Cloning of an autonomously replicating sequence (<i>ars</i>) from the <i>Bacillus subtilis</i> chromosome (1992)

Cloning of an autonomously replicating sequence (ars) from the origin region of Bacillus subtilis was previously unsuccessful because of the strong incompatibility exerted by sequences located within the oriC region. Using an ars searching vector which would be selective for drug resistance even at one copy per cell, and by cloning large fragments covering as much as possible of the oriC region, we have succeeded in isolating ars fragments from the origin region of the chromosome. The minimum essential fragment contains two DnaA-box regions (non-translatable regions containing multiple repeats of DnaA-box) separated by the dnaA gene. Neither one of the DnaA-box regions by itself showed ars activity. When constructed as oriC plasmids, the dnaA coding region could be removed without affecting ars activity. The minimum distance between the two DnaA-box regions obtained so far is 274 bp. The copy number of the oriC plasmid is estimated as one per replicating chromosome. These plasmids are unstable and tend to be lost or integrated into chromosome.
Unified Simulation of Rigid and Flexible Bodies Using Position Based Dynamics (2017)

In this paper we present a new position based approach for simulating rigid and flexible bodies with two-way coupling. This is achieved by expressing all the dynamics as constraints and running them in the same solver. Our main contribution is an accurate contact and Coulomb friction model based on a fixed point iteration of a cone complementarity problem. We formulate the problem as a nonlinear convex minimization at position level and solve it using a new accelerated form of projected Jacobi. We add elasticity to the constraints by means of regularization and show how to add more damping in a credible manner. We also use this viscoelastic model to build an accurate position-based finite element solver for soft bodies. The novelty of this solver is that it is no longer an approximation and it is based directly on the elasticity theory of continuous media.
The Fecal Microbiome in Dogs with Acute Diarrhea and Idiopathic Inflammatory Bowel Disease (2012)

Results of this study revealed a bacterial dysbiosis in fecal samples of dogs with various GI disorders. The observed changes in the microbiome differed between acute and chronic disease states. The bacterial groups that were commonly decreased during diarrhea are considered to be important short-chain fatty acid producers and may be important for canine intestinal health. Future studies should correlate these observed phylogenetic differences with functional changes in the intestinal microbiome of dogs with defined disease phenotypes.
Active Engine Mount with Piezo-Actuator for Vibration Control (1993)

<div class="htmlview paragraph">Electronics technology has made remarkable progress in recent years, and in the field of automobile noise and vibration control, it has attracted the interest of engineers due to its potential for use in the active control of vibration isolators.</div> <div class="htmlview paragraph">This report describes an investigation on reducing automobile vibration caused by engine explosion using a piezo actuator(PA) engine mount. Piezo actuator has a very high speed response but the displacement is generally very small. Therefore PA mount requires an amplitude enlargement mechanism. Here, an active mount with an enlargement mechanism incorporated was developed. For the control system, the adaptive control algorithm proposed by S. J. Elliott et al. was employed as a base, and a control rule against limitation in the power of the actuator was used in combination with it. A significant attenuation of the vibration of the dominant harmonic order in idling was achieved. The results are reported in the following pages.</div>
A simulation as a service methodology with application for crowd modeling, simulation and visualization (2014)

Crowd modeling and simulation (M&S) has been used to support the analysis of the behavior of crowds, in order to predict the impact of pedestrian movement and to test design alternatives. In recent years, crowd M&S has become more complex, and new technologies such as CAD (computer-aided design) and BIM (building information modeling) authoring tools are being used to support the process. There are challenges in adopting these technologies due to the lack of automation and integration of these tools for crowd M&S. We propose a method based on a distributed architecture with simulation in the cloud, and composition using workflows. In particular, we adopt a model-driven engineering approach to extract data from CAD/BIM authoring tools, Cell-DEVS theory for crowd modeling, simulation as a service to execute simulation remotely, and three-dimensional visualization. Finally, we present a case study for crowd evacuation, discussing the advantages of the proposed architecture. We show the advantages obtained when using distributed deployment, simulation-based design and collaborative development and we discuss how this facilitates the crowd behavior study and improves reusability in crowd M&S.
The effect of thymosin <i>β</i>4 on articular cartilage chondrocyte matrix metalloproteinase expression (2002)

Mechanical loading is paramount in regulating both the anabolic and catabolic activities of articular cartilage chondrocytes, essential for the matrix to retain its functional integrity. We have identified thymosin beta(4) as a putative mechanically regulated gene that may mediate load-enhanced synthesis and activation of matrix metalloproteinases (MMPs) 2 and 9 in articular cartilage. The objective of this study was to confirm the mechanical regulation of thymosin beta(4) and determine its effect on cartilage chondrocyte MMP production. Thymosin beta(4) mRNA expression, analysed by quantitative PCR, revealed a significant 20-fold increase in cartilage loaded for 10 min which was still evident after 30 min of loading. Treatment of primary chondrocytes with 2 and 4 micro x ml(-1) thymosin beta(4) peptide for 4 h significantly increased pro-MMP 9 expression and activation. We postulate a functional role for load-induced thymosin beta(4) in modulating the cytoskeletal organization of articular cartilage chondrocytes to affect MMP expression.