gebhart/patrepeval huggingface dataset:
Plasminogen activator inhibitor type 1 biosynthesis and mRNA level are increased by dexamethasone in human fibrosarcoma cells. (1987)

Dexamethasone increases type 1 plasminogen activator inhibitor (PAI-1) activity released from the human fibrosarcoma cell line HT-1080. We demonstrated that dexamethasone caused about 10-fold increases in the intracellular and extracellular levels of PAI-1 protein, as measured by an enzyme-linked immunosorbent assay, in the rate of PAI-1 biosynthesis, and in the PAI-1 mRNA level. The effects on PAI-1 biosynthesis and mRNA level were detectable within 4 h and were maximal 16 to 24 h after the addition of dexamethasone. Cycloheximide did not inhibit the dexamethasone-induced increases in the capacity of the cells to synthesize PAI-1 and in the PAI-1 mRNA level.
Cytocompatibility studies of an <i>in situ</i> photopolymerized thermoresponsive hydrogel nanoparticle system using human aortic smooth muscle cells (2008)

We have been investigating thermoresponsive hydrogel nanoparticle composite networks to develop photopolymerized hydrogels to deliver drugs for prevention of restenosis after angioplasty. These composite systems can form a gel under physiological conditions and release drugs in response to temperature changes. Our novel system, consisting of poly(N-isopropylacrylamide) thermoresponsive nanoparticles, photo cross-linker poly(ethylene glycol) diacrylate, and UV photoinitiator, 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone-1-one (Irgacure 2959), would be photopolymerized in situ in the presence of UV light. The focus of this study was to evaluate the effects of photoinitiator and UV exposure on human aortic smooth muscle cells (HASMCs). We found that the exposure to UV light did not significantly affect the cellular survival within doses required for photopolymerization. The photoinitiator was cytocompatible at low concentrations (< or = 0.015% w/v); however, cytotoxicity increased with increasing photoinitiator concentrations. In addition, free radicals formed in the presence of a photoinitiator and UV light caused significant levels of cell death. An antioxidant (free radical scavenger), ascorbic acid, added to the cell media, significantly improved relative cell survival but increased the hydrogel gelation time. Finally, HASMC survival when exposed to all potential cytotoxic components was also evaluated by exposing HASMCs to media incubated with our composite hydrogels. In summary, our studies show that the photoinitiator and free radicals are responsible for the cytotoxicity on HASMCs, and the addition of antioxidants can significantly reduce these harmful effects.
Analysis and optimal control of linear systems via single term Walsh series approach (1981)

This paper considers a simple method of analysis to find an optimal control of linear time-invariant and time-varying systems by the single term Walsh series approach (STWS). The value of the STWS approach in linear system analysis is established. The time varying gains in optimal control are approximated by piecewise constant gains which are easily determined by the STWS method for linear time-invariant and time-varying systems with quadratic performance criteria. The cumbersome Kronecker product is eliminated, and inversion of large matrices is avoided. Several examples are presented to demonstrate the power and simplicity of the STWS method.
Antibody Library Display on a Mammalian Virus Vector: Combining the Advantages of Both Phage and Yeast Display into One Technology (2014)

Utilizing a vaccinia virus based library technology, we previously developed an antibody discovery platform that enabled efficient selection of fully functional IgG antibodies from highly diverse immunoglobulin gene libraries expressed on the surface of mammalian cells. Recently, we have further modified this platform to enable efficient expression of a library of fully human antibodies on the surface of vaccinia virus; an enveloped mammalian virus. Similar in concept to phage display, conditions are utilized under which each vaccinia virion expresses a single antibody specificity on its surface. Various panning and magnetic bead based methods have been developed to allow screening of a library of vaccinia- MAb virions and selection of recombinant vaccinia virus encoding specific antibodies. Upon infection of mammalian cells the antibody is not only incorporated into newly produced virus, it is also displayed on the surface of the host cell. Similar to methods utilized in yeast display, the cells displaying vaccinia encoded antibody can also be selected using a combination of magnetic beads and cell sorting, and the virus encoding the specific antibody heavy and light chains readily recovered and analyzed. This technology allows for rapid high throughput selection of vaccinia-MAb virions in a cell free panning system, followed by cell based screening for high specificity and fine selection of optimal antibodies.
Reduction of Plasma Fibrinogen, Immunoglobulin G, and Immunoglobulin M Concentrations by Immunoadsorption Therapy with Tryptophan and Phenylalanine Adsorbents (1996)

Immunoadsorption (IA) therapy with tryptophan (TR-350) or phenylalanine (PH-350) adsorbents has been used to reduce the concentration of serum antibodies in human lymphocyte antigen (HLA)-immunized patients. Other forms of plasma purification have been reported to reduce the level of fibrinogen, which affects the blood properties. In this study we investigated the effects of IA therapy using both adsorbents on plasma fibrinogen and immunoglobulins G and M in 13 patients (8 patients were treated with TR-350, and 5 patients were treated with PH-350). During each session I plasma volume (2.8 +/- 0.4 L of plasma) was processed through the immunocolumn and then returned to the patient together with the blood cells. Compared with the pretreatment values, the plasma fibrinogen, IgG, and IgM concentrations were significantly reduced after IA therapy (p < 0.01 for TR-350; p < 0.04 for PH-350). There was a positive correlation between the degree of reduction of plasma proteins and the number of IA treatments given. A nonparametric test (Wilcoxon's signed-rank test or the Mann-Whitney test) was used for statistical analysis. We conclude from our study that IA therapy effectively lowers the plasma levels of fibrinogen, IgG, and IgM and thus can be considered a valuable alternative to other blood purification methods.
Evaluation of Novel Phosphoramidate ProTides of the 2′-Fluoro Derivatives of a Potent Anti-Varicella Zoster Virus Bicyclic Nucleoside Analogue (2010)

ProTide-based kinase bypass is not successful in this case.
Purification and characterization of aryl acylamidase from <i>Nocardia globerula</i> (1991)

Aryl acylamidase was purified from an extract of N-acetyl-o-toluidine-induced cells of Nocardia globerula IFO 13510 in ten steps. The purified enzyme appeared to be homogeneous from analysis by polyacrylamide gel electrophoresis. The enzyme has a molecular mass of approximately 126 kDa and consists of two subunits which are identical in molecular mass. The purified enzyme catalyzed the hydrolysis of N-acetyl-o-toluidine to o-toluidine and acetic acid at a rate of 47.7 mumol.min-1.mg-1 at 35 degrees C. It also catalyzed the hydrolysis of various anilide derivatives and esters, as well as the transfer of an acetyl group to aniline as an acetyl acceptor. The purified enzyme was sensitive to thiol reagents such as HgCl2 and p-chloromercuribenzoate. The amino-terminal sequence (28 amino acid residues) of the enzyme was determined. Based on the substrate specificity of this enzyme, the pathway intermediates involved in the conversion of n-acetyl-o-toluidine to 4'-hydroxy-N-acetyl-o-toluidine are discussed.
Solar angioedema: an uncommonly recognized condition? (2005)

Solar urticaria is a well defined although uncommon photosensitivity disorder, and is said to be the underlying cause of chronic urticaria in approximately 0.5% cases. In contrast, solar angioedema is seldom reported. We describe two patients with postulated solar angioedema, associated with clinical and/or phototest features of solar urticaria. Recognition of solar provocation of angioedema has important consequences for patient management.
Leg Morbidity and Function Following Fibular Free Flap Harvest (1997)

Over a period of 3 years, 50 consecutive free fibular flaps for mandibular reconstruction were performed on 47 patients. In 38 patients (81%) a skin paddle was included with the flap to provide either mucosal lining or skin cover; in 9 patients (19%) bone alone was used. Thirty-one patients (66%) required a skin graft to close the donor defect in the leg. Donor leg morbidity and function were determined by patient questionnaire and by physical examination. Forty-one donor sites in 40 patients were available for long-term follow-up. The follow-up ranged from 4 to 39 months with an average of 17 months. Immediate postoperative infection occurred in the donor site of 1 patient (2%) and required additional surgery. There was no other immediate donor site complications when closure required skin grafting. Eleven patients (27%) had late donor site morbidity, consisting of motor weakness of the great toe in 5 patients, ankle instability and/or stiffness in 3 patients, donor site pain in 1 patient, and edema in 2 patients. All complications were graded as mild in severity by the patient and by the examiner. In this series, although most donor site defects required skin grafting, short- and long-term morbidity was minimal. After a short rehabilitation period, all patients were fully able to engage in all daily and recreational activities.
The Effects of Resistive Loading of "TEM" Horns (1982)

A short transverse electromagnetic (TEM) horn with continuously tapered resistive loading was developed for directional reception or transmission of picosecond pulses with minimal distortion. It was found to be broadband and nondispersive with a low VSWR. The receiving transient response of the resistively loaded "TEM" horn indicates that the waveform of a 70-ps impluse is well preserved. The theoretical analyses using the method of moments and the fast Fourier transform (FFT) technique were performed and agreed well with time-domain measurements.
Adaptive linear robot control for tracking and grasping a dynamic object (2003)

Versatile vision systems are being employed increasingly for automated visual feedback intelligent robotic control to perform complex manufacturing tasks especially for tracking and grasping dynamic objects a the conveyor by generating the optimum-tracking trajectory for the robot. Currently automated visual feedback robot systems use vision and robot system as separate tools. The best solution for these kind of problems still can not be implemented successfully with ease. Therefore, we tried to develop a new single adaptive prediction and execution algorithm for the picking and placing of dynamic objects in real time in a robot work-cell by integrating a CCD camera with frame grabber, artificial neural network, an optical flow technique and an industrial robot into a single application. The implementation of this proposed system is done only in one dimension because of the time constraint. After the robot learning process, it is shown that a KUKA robot is capable of adaptively tracking and intercepting dynamic objects at an optimal rendezvous point on the conveyor accurately in real time.
Supporting parallel soft real-time applications in virtualized environment (2013)

The prevalence of multicore processors and virtualization technology enables parallel soft real-time applications to run in virtualized environment. However, current hypervisors do not provide adequate support for them because of soft real-time constraints and synchronization problems, which result in frequent deadline misses and serious performance degradation. In this paper, we propose a novel parallel soft real-time scheduling algorithm which addresses them well, and implement a parallel soft real-time scheduler, named Poris, based on Xen. Our evaluation shows that Poris shortens the execution time of PARSEC benchmark by up to 44.12% compared to Credit scheduler.
Collaborative multi-output Gaussian processes (2014)

We introduce the collaborative multi-output Gaussian process (GP) model for learning dependent tasks with very large datasets. The model fosters task correlations by mixing sparse processes and sharing multiple sets of inducing points. This facilitates the applica-tion of variational inference and the deriva-tion of an evidence lower bound that decom-poses across inputs and outputs. We learn all the parameters of the model in a sin-gle stochastic optimization framework that scales to a large number of observations per output and a large number of outputs. We demonstrate our approach on a toy prob-lem, two medium-sized datasets and a large dataset. The model achieves superior per-formance compared to single output learn-ing and previous multi-output GP models, confirming the benefits of correlating spar-sity structure of the outputs via the inducing points. 1
Antioxidative Phenolic Compounds Isolated from Almond Skins (<i>Prunus amygdalus</i> Batsch) (2002)

Nine phenolic compounds were isolated from the ethyl acetate and n-butanol fractions of almond (Prunus amygdalus) skins. On the basis of NMR data, MS data, and comparison with the literature, these compounds were identified as 3'-O-methylquercetin 3-O-beta-D-glucopyranoside (1); 3'-O-methylquercetin 3-O-beta-D-galactopyranoside (2); 3'-O-methylquercetin 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (3); kaempferol 3-O-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside (4); naringenin 7-O-beta-D-glucopyranoside (5); catechin (6); protocatechuic acid (7); vanillic acid (8); and p-hydroxybenzoic acid (9). All of these compounds have been isolated from almond skins for the first time. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities for compounds 1-9 were determined. Compounds 6 and 7 show very strong DPPH radical scavenging activity. Compounds 1-3, 5, 8, and 9 show strong activity, whereas compound 4 has very weak activity.
Microemulsion phase behavior of anionic‐cationic surfactant mixtures: Effect of tail branching (2006)

Abstract This research evaluated middle‐phase microemulsion formation by varying the mole ratio of anionic and cationic surfactants in mixtures with four different oils (trichloroethylene, n ‐hexane, limonene, and n ‐hexadecane). Mixtures of a double‐tailed anionic surfactant (sodium dihexyl sulfosuccinate, SDHS) and an unbalanced‐tail (i.e., doubletailed with tails of different length) cationic surfactant (benzethonium chloride, BCl) were able to form microemulsions without alcohol addition. The amount of NaCl required to form the middle‐phase microemulsion decreased dramatically as an equimolar anionic‐cationic surfactant mixture was approached. Although the mixture of anionic and cationic surfactants demonstrated a higher critical microemulsion concentration (cμc) compared to the anionic surfactant alone, the Winsor Type IV single‐phase microemulsion started at lower surfactant concentrations for the anionic‐cationic mixture than for the anionic surfactant alone. Under optimum middlephase microemulsion conditions, mixed anionic‐cationic surfactant systems solubilized more oil than the anionic surfactant alone. Pretreatment detergency studies were conducted to test the capacity of these mixed surfactant systems to remove oil form fabrics. It was found that anionic‐rich mixed surfactant formulations yielded the largest oil removal, followed by cationic‐rich systems.
Facile Synthetic Methods for 3- and 5-Trifluoromethyl-4-trifluoroacetylpyrazoles and Their Conversion into Pyrazole-4-carboxylic Acids (1992)

3-and 5-Trifluoromethyl-4-trifluoroacetylpyrazales (b and 5) were easily synthesized in excellent yields by reaction of 6,&bis(trifluoro-acety1)vinyl ethers 1, sulfides 2, and -mines 3 with hydrazines.Hydrolysis of these compaunds (b and 5) with aqueous potassium hydroxide gave the corresponding pyrazole-4-carboxylic acids ( 6 and 7) in high yields.In the course of our extensive investigations an the nucleophilic substitutions at olefinic carbon it was found that &B-bis(trifluoroacety1)vinyl i-butyl ether 1 readily reacts with various thiols and mines under very mild conditions to give the corresponding 0-S and 0-N exchanged products (e.g.. 2 and 3) in high yields.6As an extension of this work, we used this type of nucleophilic exchange reaction and subsequent cycladehydration with bifunctional N-nucleophiles such as hydrazines to prepare 3-and 1723 cm-l; l~-nmr 8.39 (1H, s, H-5), 7.72-7.32(5H.s, C6H5).Anal.Calcd for Cl2HgN20F6: C, 46.77; H, 1.
FERTILIZERS AND LIME FOR NORTH AUCKLAND SOILS (1967)

NORTH AUCKLAND is covered by a large variety of soils. These have developed on sandstones, mudstones, andesitic rocks, basalt, volcanic ash and mixtures of these materials. Further, on each type of parent material, time and environment have combined to create a range or "suite" of soils. The term and concept of soil "suites" was developed by Taylor (1952), formerly Director of the New Zealand Soil Bureau. Dr Taylor's system of grouping these soils has been of very great help in planning field and laboratory experiments. Table 1 outlines a simplified model of a soil suite developed on sandstone. Natural fertility is highest on the A type soils, very much lower on the B and C type soils and very low on the D and E soils. The last two (D and E) and part of the C soils used to be dug for kauri gum. Hence they are known as gumland soils.
Channel Characterization for 700 MHz DSRC Vehicular Communication (2010)

Adapting OFDM for vehicular communication requires extensive knowledge of anticipated multipath and Doppler environments. We present a GPS‐enabled channel sounding system built and used to conduct a channel measurement campaign. Tests conducted at the 700 MHz band in and around downtown Ann Arbor, Michigan, explored various vehicle‐to‐vehicle and vehicle‐to‐roadside channel scenarios. The measured channel metrics are used to quantify the effects on guard interval, packet duration, and subcarrier spacing for a functional OFDM system at 700 MHz. This paper is one of the first to present vehicular‐based channel‐modeling results from measured data in the 700 MHz band.
Automatic video-based human motion analyzer for consumer surveillance system (2009)

With the continuous improvements in video-analysis techniques, automatic low-cost video surveillance gradually emerges for consumer applications. Video surveillance can contribute to the safety of people in the home and ease control of home-entrance and equipment-usage functions. In this paper, we study a flexible framework for semantic analysis of human behavior from a monocular surveillance video, captured by a consumer camera. Successful trajectory estimation and human-body modeling facilitate the semantic analysis of human activities and events in video sequences. An additional contribution is the introduction of a 3-D reconstruction scheme for scene understanding, so that the actions of persons can be analyzed from different views. The total framework consists of four processing levels: (1) a preprocessing level including background modeling and multiple-person detection, (2) an object-based level performing trajectory estimation and posture classification, (3) an event-based level for semantic analysis, and (4) a visualization level including camera calibration and 3-D scene reconstruction. Our proposed framework was evaluated and has shown its good quality (86% accuracy of posture classification and 90% for events) and effectiveness, as it achieves a near real-time performance (6-8 frames/second).
Synthetic Approaches to Rapamycin. 3. Synthesis of a C1-C21 Fragment (1996)

Key steps in two alternative approaches to the C10-C21 fragments 4 and 17 of the immunosuppressant rapamycin employ catalytic asymmetric allylation and catalytic asymmetric hydrogenation to introduce the first stereogenic centre at C14. Appendage of C1-C9 via trapping of an a-oxoketene intermediate generates the C1-C21 fragment 5.
Synthesis and Antibacterial Activity of Novel Pleuromutilin Derivatives (2015)

In this study we describe the design, synthesis, and antibacterial activity of novel pleuromutilin analogs. A series of new compounds containing piperazine and alkylamino or arylamino groups was synthesized. The new compounds were characterized via (1)H-NMR, (13)C-NMR, Fourier transform (FT)-IR and MS, and were further evaluated for their in vitro activity against seven Gram-positive, and one Gram-negative, pathogens. Antibacterial data revealed that all compounds exhibited moderate to good antibacterial activities against sensitive Gram-positive pathogens. Specifically, 9d displayed the best activity: its activity to Staphylococcus aureus (ATCC25923) is 0.125 µg/mL, which is equal to the control compound tiamulin. The antibacterial activities of 9d to Streptococcus suis (minimum inhibitory concentration (MIC) of 2 µg/mL), Streptococcus agalactiae (MIC of 0.5 µg/mL), and Streptococcus dysgalactiae (MIC of 0.5 µg/mL) were also excellent compared with the control drug erythromycin (MIC of >128 µg/mL). The binding modes of these compounds with active sites were calculated using the programs of Molecular Operating Environment (MOE) and Pymol.
The Prospects For MBA Enrollments, 1978-1990 (1978)

Beyond doubt realistic projections of MBA enrollment are needed, but the available estimates are unreliable. This study provides an alternative methodology for projecting MBA's to 1990. The National Center for Education Statistics (NCES) projects MBA's by compounding a series of five past ratios. The NCES methodology projects a decline in MBA's beginning in 1984-85. Our methodology examines variables reflecting costs, benefits, institutional factors and changes in the age-eligible population. The model that best explains MBA output of the past 12 years and interrelates future conditions, projects MBA enrollments to increase at a decreasing rate through 1990-91. Also, there will be a continuation of the enrollment shift from the traditional full-time MBA program to part-time evening MBA programs on urban campuses.
Development and Characterization of Biodegradable/Edible Wheat Protein Films (1997)

ABSTRACT Biodegradable and edible films were prepared from three types of wheat flours: commercial bread, hard red winter, and soft white. Films were produced at two pH values (4 and 11) and tested for oxygen permeability as related to temperature. Films were also produced with a cross‐linked agent and tested for tensile strengths. Oxygen permeability was 5.9 × 10 −20 to 18.5 × 10 −20 m 3 O 2 m m −2 s −1 Pa −1 similar to values for commercial nylon. The oxygen permeability activation energy varied from 9.1 to 14.5 kcal mol −1 , depending on type of flour and pH did not affect oxygen permeability. Presence of the cross‐linking agent increased the strength of films and elongation at break ranged from 490% to 640%, while tensile stress at break ranged from 25.8 × 10 −3 kg m −2 to 44.1 × 10 −3 kg m −2 , lower than commercial nylon.
Remotely Operated Underwater Thermal Cutting Processes for the Decommissioning of Large North Sea Platforms (2002)

A study has demonstrated the automation of underwater thermal cutting processes for remote decommissioning operations. The first phase of cutting trials evaluated five cutting processes underwater (air plasma, oxy-hydrogen, flux-cored arc, oxy-petrol and Kerrie cable systems). In the second phase, three manual cutting systems (oxy-petrol, “Broco” type system and Kerrie cable) were adapted for operation by a manipulator arm of the type commonly used offshore on Remotely Operated Vehicles. This manipulator, which usually requires a human operator, was interfaced to computer simulation and control software. The results of underwater cuts on steel plate using these systems are discussed, with applicability to offshore decommissioning. Ongoing work using other novel thermal cutting processes is also described.