gebhart/patrepeval huggingface dataset:
A fluorescent resonant energy transfer–based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows (2002)

Approaches with high spatial and temporal resolution are required to understand the regulation of nonmuscle myosin II in vivo. Using fluorescence resonance energy transfer we have produced a novel biosensor allowing simultaneous determination of myosin light chain kinase (MLCK) localization and its [Ca2+]4/calmodulin-binding state in living cells. We observe transient recruitment of diffuse MLCK to stress fibers and its in situ activation before contraction. MLCK is highly active in the lamella of migrating cells, but not at the retracting tail. This unexpected result highlights a potential role for MLCK-mediated myosin contractility in the lamella as a driving force for migration. During cytokinesis, MLCK was enriched at the spindle equator during late metaphase, and was maximally activated just before cleavage furrow constriction. As furrow contraction was completed, active MLCK was redistributed to the poles of the daughter cells. These results show MLCK is a myosin regulator in the lamella and contractile ring, and pinpoints sites where myosin function may be mediated by other kinases.
Regulation of IGFBP secretion and modulation of cell growth in MDBK cells. (1993)

The ability of IGF binding proteins (IGFBP) to modulate cell growth and IGF-I responsiveness of epithelial cells was examined using the Madin-Darby bovine kidney (MDBK) cell line. The predominant IGFBP present in conditioned media (CM) of untreated cells was found to be IGFBP-2. Following exposure to forskolin, the abundance of IGFBP-2 in CM was decreased, while IGFBP-3 and -4 were induced. These changes corresponded with alterations in mRNA abundance. Growth of MDBK cells in serum-free media was stimulated by addition of 2.5 to 50 ng/ml of IGF-I in a dose responsive manner. Coincubation with equimolar amounts of IGF-I and exogenous bovine IGFBP-3 potentiated the growth response observed with IGF-I alone. In order to alter endogenous IGFBP-3 secretion, cells were exposed to transfection with an expression vector containing sense IGFBP-3 cDNA. Following selection and amplification with methotrexate, cells underwent a permanent alteration in cell morphology, exhibiting characteristics of transporting epithelia. This was associated with secretion of IGFBP-3 under basal conditions. Secretion of IGFBP-3 was due to expression of endogenous IGFBP-3 and not to expression of the transgene. Cells expressing IGFBP-3 under basal conditions grew slower in serum, but were more responsive to 100 ng/ml of IGF-1 in serum-free media compared to wild-type MDBK cells. The role of IGFBP-3 in mediating these responses requires further study.
Studies of the bone marrow in polycythemia vera and the evolution of myelofibrosis and second hematologic malignancies. (1986)

The PVSG study is unique in that it is prospective and composed of 432 patients randomized to three treatment arms. This study also provides the opportunity for serial studies of numerous sequential biopsies. Large numbers of cases with sequential biopsies covering the entire long course are essential to appreciate the full spectrum of tissue changes in this disease. The PVSG was initiated in 1967 and in mid-1985 approximately one third of the patients are alive and on protocol. For these reasons, the results must still be considered preliminary. Pretreatment biopsies from patients randomized in the PVSG have been analyzed for total cellularity, megakaryocyte concentration, and reticulin content. Considerable variation in these elements was found in these biopsies. Sequential posttreatment biopsies from these patients have also been studied and correlated with the clinical course of the disease. None of the morphologic parameters analyzed was shown to be of prognostic significance. Early in the course of PV the marrow reticulin content is almost always normal. The length of the developmental stage is unknown and the precise timing of the clinical onset may be difficult. Therefore, the 11% of patients that showed a significant increase in reticulin on initial evaluation may have had PV longer than was indicated clinically. If large numbers of sequential biopsies are studied, an increase in reticulin content can frequently be demonstrated during the active phase of the disease and before the onset of the spent phase. Currently 39 patients (9%) have developed the spent phase, or PPMM. PPMM occurred in about the same incidence in the patients treated with myelosuppressive therapy as by phlebotomy alone, the spent phase occurring in 16 patients treated by phlebotomy alone, 11 with chlorambucil, and 12 with 32P. The course of the reticulin fibrosis is slowly progressive. There is some evidence for regression in a few patients in the erythrocytotic phase, but sampling variation cannot be completely ruled out. At this time in the study, AL has developed in 37 patients (8.6%). The incidence of AL is quite low in the phlebotomy group (three cases). Presumably this represents the natural incidence in PV unmodified by therapeutic agents. The frequency is approximately equal and quite high in the chlorambucil and 32P groups. There are 19 cases in the chlorambucil-treated group and 15 in the 32P-treated group. The leukemias that developed in the PV patients occurred either de novo or following PPMM.(ABSTRACT TRUNCATED AT 400 WORDS)
Correlation of Radiographic Imaging and Histopathology Following Cryoablation and Radio Frequency Ablation for Renal Tumors (2008)

We observed a poor correlation between radiographic imaging and pathological analysis. We recommend post-radio frequency ablation followup biopsy due to the significant risk of residual renal cell cancer without radiographic evidence, although to our knowledge the clinical significance of these viable cells remains to be determined. In contrast, radiographic images of renal lesions treated with cryotherapy appeared to correlate adequately with corresponding histopathological findings in our series.
Nitrite/nitrate (NOX) and type II phospholipase A2, leukotriene B4, and platelet-activating factor levels in patients with septic shock. (1996)

We measured serum concentrations of nitrite/nitrate (NOX), type II phospholipase A2 (PLA2), leukotriene B4 (LTB4), and platelet-activating factor (PAF) in patients with sepsis. These findings were compared between patients with and without septic shock. Serum concentrations of NOX, type II PLA2, LTB4, and PAF acetylhydrolase (PAF-AH) were significantly higher in the group with septic shock (P < 0.0001; P = 0.0060; P = 0.0052; P = 0.0052), indicating the severity of the disease. There were significant correlations between the serum NOX level and serum levels of type II PLA2, LTB4, and PAF-AH (r = 0.6890, P < 0.0001; r = 0.3755, P = 0.0409; r = 0.5095, P = 0.0040, respectively). It is speculated that LTB4 and PAF, both produced with type II PLA2, interact with each other and are involved in the deterioration of pathologic features associated with sepsis. Furthermore, nitric oxide (NO) and eicosanoids interact to play an important role in vascular dilatation during septic shock.
Disulfide bridges in tomato pectinesterase: Variations from pectinesterases of other species; conservation of possible active site segments (1992)

Analysis of tomato pectinesterase by carboxymethylation, with and without reduction, shows that the enzyme has two intrachain disulfide bridges. Analysis of fragments obtained from the native enzyme after digestion with pepsin identified bridges connecting Cys-98 with Cys-125, and Cys-166 with Cys-200. The locations of disulfide bridges in tomato pectinesterase are not identical to those in three distantly related pectinesterases (18-33% residue identities) from microorganisms. However, one half-Cys (i.e., Cys-166) position is conserved in all four enzymes. Sequence comparisons of the overall structures suggest a special importance for three short segments of the entire protein. One segment is at the N-terminal part of the tomato pectinesterase, another in the C-terminal portion near the distal end of the second disulfide loop, and the third segment is located in the central part between the two disulfide bridges. The latter segment, encompassing only 40 residues of the entire protein, appears to high-light a functional site in a midchain segment.
MARKERS OF ALLOGRAFT VIABILITY IN THE RAT RELATIONSHIP BETWEEN TRANSPLANTATION VIABILITY AND LIVER FUNCTION IN THE ISOLATED PERFUSED LIVER (1987)

The relationship between transplant viability and liver function has been examined. Wistar rat livers were preserved at 4 degrees C for increasing intervals and then transplanted into Wistar rat recipients. Two critical times were identified, the longest preservation period with 100% transplantation success (4 hr) and the shortest preservation period with 100% transplant failure (8 hr). The comparable critical times were also identified in livers preserved at 37 degrees C (1 hr and 2 hr). Liver functions were studied by the isolated perfused liver technique in other rat livers stored at 4 degrees C or 37 degrees C for the critical times. Two liver function tests, AST and LDH concentration in perfusate, discriminated between viable and nonviable livers across as well as within preservation groups. AST gave the best separation between viable and nonviable livers. Some functions such as ALT concentration in perfusate separated viable from non viable allografts only within preservation groups. Other liver functions were more sensitive to preservation temperature than allograft viability. Oxygen consumption after cold preservation for either critical time was about twice control levels. Urea production was far below control levels in warm-preserved livers but almost normal in cold-preserved livers. Our results indicate that AST release into perfusate can be used as a screening technique to optimize preservation methods, reserving transplantation for confirming the most promising results.
Synthesis of perbromobenzoic acids and perbromobenzenes from aromatic carboxylic acids by permercuration and bromodemercuration (1977)

Permercuration of the benzoic acids XC6H4CO2H (X = o-Me, F, Cl, or Br; m- Me, F, Cl, Br, CF3, NO2 or OMe; or p-Me, F, Cl, Br, CF3 or NO2) and 2,6- X2C6H3CO2H (X = Me, Cl, or Br) with molten mercuric trifluoroacetate at c. 180-245° followed by bromodemercuration gave the corresponding perbromobenzoic acids XC6Br4CO2H or 2,6-X2C6Br3CO2H, together with the corresponding perbromobenzenes C6Br5X or m-X2C6Br4 which were formed owing to decarboxylation under permercuration conditions. Similar treatment of the acids XC6H4CO2H (X = o-NO2, CF3, or OMe; or p-OMe) and 2,6-F2C6H3CO2H gave only the appropriate perbromobenzenes. Possible mechanisms for permercuration induced decarboxylation are proposed on the basis of the effect of substituents on yields of perbromobenzoic acids and perbromobenzenes. Decarboxylation occurs more widely under permercuration conditions than on pyrolysis of mercuric carboxylates. Regiospecific mercuration meta to the carboxyl group and not decarboxylation occurred on thermal decomposition of mercuric p-methoxybenzoate.
Oct-tree terrain modelling methods for terrain reference navigation systems (1996)

Abstract This paper describes the use of oct-trees to represent digitised terrain elevation data (DTED) in terrain reference navigation (TRN) systems. Oct-trees provide a regular method to represent digitised terrain data where the level of detail of the terrain is encoded in the tree structure. The use of oct-trees also provides a basis for a significant reduction in the on-line storage requirement for DT-EDs. A method of encoding using Morton ordering is introduced which allows DTEDs to be accessed as oct-trees and quad-trees where the tree structures are represented as pointerless structures. Algorithms to construct and access terrain oct-trees are presented which form a set of access primitives for tree operations required in TRN applications. The paper concludes with examples to illustrate the efficiency of the methods described in the paper for two DTEDs, in terms of performance of the tree access operations and reductions in storage.
Cache-aware load balancing of data center applications (2019)

Our deployment of cache-aware load balancing in the Google web search backend reduced cache misses by ~0.5x, contributing to a double-digit percentage increase in the throughput of our serving clusters by relieving a bottleneck. This innovation has benefited all production workloads since 2015, serving billions of queries daily. A load balancer forwards each query to one of several identical serving replicas. The replica pulls each term's postings list into RAM from flash, either locally or over the network. Flash bandwidth is a critical bottleneck, motivating an application-directed RAM cache on each replica. Sending the same term reliably to the same replica would increase the chance it hits cache, and avoid polluting the other replicas' caches. However, most queries contain multiple terms and we have to send the whole query to one replica, so it is not possible to achieve a perfect partitioning of terms to replicas. We solve this via a voting scheme, whereby the load balancer conducts a weighted vote by the terms in each query, and sends the query to the winning replica. We develop a multi-stage scalable algorithm to learn these weights. We first construct a large-scale term-query graph from logs and apply a distributed balanced graph partitioning algorithm to cluster each term to a preferred replica. This yields a good but simplistic initial voting table, which we then iteratively refine via cache simulation to capture feedback effects.
Comparative study of double ion implant Ti salicide and pre-amorphization implant Co salicide for ultra-large-scale integration applications (2002)

We have investigated the interesting double ion implant (DII) Ti–salicide and pre-amorphization implant (PAI) Co–salicide techniques for ultra-large-scale integration (ULSI) applications. The DII technique is combined with germanium (or arsenic) PAI and Si ion-mixing processes. The sheet resistances both of n+ and p+ polysilicons are decreased when the DII Ti–salicide and PAI Co–salicide techniques are used. Moreover, the incomplete phase transformation of Ti–salicide is not observed in 0.2 μm wide polysilicon devices with the Ge DII process. Furthermore, the n+/p-well junction leakage current is reduced when the Si ion-mixing process is used. Experimentally, based on the studied DII Ti–salicide and PAI Co–salicide techniques, high-performance 0.2 μm CMOS devices have been successfully fabricated.
ChemInform Abstract: MAGNETIC CHARACTERISTICS OF RCO2‐XFEX HYDRIDES (R = TB, DY) (1982)

Abstract Die Laves‐Phasen RC0, ..x F ex (R: Tb, Dy; x: 0, 0.6, 2) absorbieren 3‐3.5 H‐Atome pro Formeleinheit, was zu einem starken Anstieg der Elementarzellenvolumina (20‐25%) ohne Struktur‐ änderung führt.
Quantifying long-term microelectrode array functionality using chronic<i>in vivo</i>impedance testing (2012)

Long-term acquisition of high-quality neural recordings is a cornerstone of neuroprosthetic system design. Mitigating the experimental variability of chronically implanted arrays has been a formidable task because the sensor recording sites can be influenced by biotic and abiotic responses. Several studies have implicated changes in electrical interface impedance as a preliminary marker to infer electrode viability. Microelectrode impedance plays an important role in the monitoring of low amplitude and high-resolution extracellular neural signals. In this work, we seek to quantify long-term microelectrode array functionality and derive an impedance-based predictor for electrode functionality that correlates the recording site electrical properties with the functional neuronal recordings in vivo. High temporal resolution metrics of this type would allow one to assess, predict, and improve electrode performance in the future. In a large cohort of animals, we performed daily impedance measurements and neural signal recordings over long periods (up to 21 weeks) of time in rats using tungsten microwire arrays implanted into the somatosensory cortex. This study revealed that there was a time-varying trend in the modulation of impedance that was related to electrode performance. Single units were best detected from electrodes at time points when the electrode entered into the 40-150 KΩ impedance range. This impedance trend was modeled across the full cohort of animals to predict future electrode performance. The model was tested on data from all animals and was able to provide predictions of electrode performance chronically. Insight from this study can be combined with knowledge of electrode materials and histological analysis to provide a more comprehensive predictive model of electrode failure in the future.
Novel Acidic 11β-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) Inhibitor with Reduced Acyl Glucuronide Liability: The Discovery of 4-[4-(2-Adamantylcarbamoyl)-5-<i>tert</i>-butyl-pyrazol-1-yl]benzoic Acid (AZD8329) (2012)

Inhibition of 11β-HSD1 is viewed as a potential target for the treatment of obesity and other elements of the metabolic syndrome. We report here the optimization of a carboxylic acid class of inhibitors from AZD4017 (1) to the development candidate AZD8329 (27). A structural change from pyridine to pyrazole together with structural optimization led to an improved technical profile in terms of both solubility and pharmacokinetics. The extent of acyl glucuronidation was reduced through structural optimization of both the carboxylic acid and amide substituents, coupled with a reduction in lipophilicity leading to an overall increase in metabolic stability.
Provenance determination of sapphires and rubies using laser-induced breakdown spectroscopy and multivariate analysis (2015)

Determination of gem provenance is a topic of research in the gemological community for financial, security, and societal reasons. Laser-induced breakdown spectroscopy (LIBS) and multivariate analysis have the potential to revolutionize the field of gem provenance. This study acquired LIBS spectra from 569 rough sapphire and ruby specimens from 21 localities in 11 countries. The spectra were analyzed using the multivariate technique partial least-squares regression (PLSR) in separate algorithms for sapphires and rubies. Each algorithm consists of a series of PLS models. Each model compares the spectra from a locality of interest to the spectra from all other localities in the database. Success rates, as determined by the percent of correct provenance identifications, are 98.9% (sapphire) and 96.0% (ruby) for country of origin and 97.9% (sapphire) and 95.4% (ruby) for deposit of origin. Individual deposits are not recognized by the concentrations of a few elements; rather, the unique compositional signature of each deposit consists of the ratios of many elements, primarily Ca, Zr, Fe, Ba, Mt, Ti, Sr, Si, Cr, H, C, and Li, some of which may reside in inclusions. This work demonstrates that determination of country or deposit of origin may be related to a quantitative measure with a high level of success.
A dynamic and context-aware semantic mediation service for discovering and fusion of heterogeneous sensor data (2013)

Sensors play an increasingly critical role in capturing and distributing observations of phenomena in our environment. The vision of the semantic sensor web is to enable the interoperability of various applications that use sensor data provided by semantically heterogeneous sensor services. However, several challenges still need to be addressed to achieve this vision. More particularly, mechanisms that can support context-aware semantic mapping and that can adapt to the dynamic metadata of sensors are required. Semantic mapping for the sensor web is required to support sensor data fusion, sensor data discovery and retrieval, and automatic semantic annotation, to name only a few tasks. This paper presents a context-aware ontology-based semantic mediation service for heterogeneous sensor services. The semantic mediation service is context-aware and dynamic because it takes into account the real-time variability of thematic, spatial, and temporal elements that describe sensor data in different contexts. The semantic mediation service integrates rule-based reasoning to support the resolution of semantic heterogeneities. An application scenario is presented showing how the semantic mediation service can improve sensor data interpretation, reuse, and sharing in static and dynamic settings.
[The effect of HPV16E7 DNA vaccine transdermal delivery with microneedle array]. (2008)

The DNA vaccine combined administering with microneedle array might get across the microchannels generated by microneedle arrays in vitro and induce humoral and cellular immune response in vivo.
Wave-equation reflection tomography: annihilators and sensitivity kernels (2006)

In seismic tomography, the finite frequency content of broad-band data leads to interference\neffects in the process of medium reconstruction, which are ignored in traditional ray theoretical\nimplementations. Various ways of looking at these effects in the framework of transmission\ntomography can be found in the literature. Here,we consider inverse scattering of bodywaves to\ndevelop a method of wave-equation reflection tomography with broad-band waveform data—\nwhich in exploration seismics is identified as a method of wave-equation migration velocity\nanalysis. In the transition from transmission to reflection tomography the usual cross correlation\nbetween modelled and observedwaveforms of a particular phase arrival is replaced by the action\nof operators (annihilators) to the observed broad-bandwavefields. Using the generalized screen\nexpansion for one-way wave propagation, we develop the Fr´echet (or sensitivity) kernel, and\nshow how it can be evaluated with an adjoint state method. We cast the reflection tomography\ninto an optimization procedure; the kernel appears in the gradient of this procedure.We include\na numerical example of evaluating the kernel in a modified Marmousi model, which illustrates\nthe complex dependency of the kernel on frequency band and, hence, scale. In heterogeneous\nmedia the kernels reflect proper wave dynamics and do not reveal a self-similar dependence\non frequency: low-frequency wave components sample preferentially the smoother parts of\nthe model, whereas the high-frequency data are—as expected—more sensitive to the stronger\nheterogeneity.We develop the concept for acoustic waves but there are no inherent limitations\nfor the extension to the fully elastic case.
RESIDENTIAL MOBILITY WITHIN AUSTRALIAN CITIES BY C. A. MAHER, ABS CENSUS MONOGRAPH SERIES, CATALOGUE 3410.0. 114 PAGES, 32 MAPS & MATRIX TABLES, 1984 (1985)

(1985). RESIDENTIAL MOBILITY WITHIN AUSTRALIAN CITIES BY C. A. MAHER, ABS CENSUS MONOGRAPH SERIES, CATALOGUE 3410.0. 114 PAGES, 32 MAPS & MATRIX TABLES, 1984. Australian Planner: Vol. 23, No. 1, pp. 26-28.
Software For Development Of Expert Systems (1990)

Modular software system helps create efficient artificial-intelligence computer programs. STAR*TOOL system: Set of high-level software tools; assists programmers in creation of efficient knowledge-based software systems. Provides language and capabilities for compilation of application programs written in Common LISP. Features modularity enabling elimination of unnecessary capabilities from final application program and achieves greater computing performance. Runs on any computer that supports Common LISP and has sufficient memory. Provides programmer with necessary software tools to build wide variety of reasoning and inference engines for such applications as planning, diagnosis and analysis, and simulation.
Acupuncture Therapy. Current Chinese Practice. (1973)

Acupuncture therapy : current Chinese practice , Acupuncture therapy : current Chinese practice , کتابخانه دیجیتال جندی شاپور اهواز