gebhart/patrepeval huggingface dataset:
A disulfone-based approach to ansa-titanocenes: synthesis of ethylene bis(2-indenyl)titanium dichloride (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA disulfone-based approach to ansa-titanocenes: synthesis of ethylene bis(2-indenyl)titanium dichlorideMichael H. Nantz, Shawn R. Hitchcock, Scott C. Sutton, and Michael D. SmithCite this: Organometallics 1993, 12, 12, 5012–5015Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/om00036a049https://doi.org/10.1021/om00036a049research-articleACS PublicationsRequest reuse permissionsArticle Views154Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cyclin-B1-mediated inhibition of excess separase is required for timely chromosome disjunction (2006)

Separase, the cysteine protease that cleaves cohesin and thereby triggers chromosome disjunction, is inhibited by both securin- and phosphorylation-dependent cyclin B1 binding. Using a novel phosphorylation-specific antibody, we show that mitotic-specific phosphorylation of human separase on S1126 is required to establish, but not maintain, cyclin B1 binding. Cells expressing a non-phosphorylatable S1126A mutant maintain cohesion early in mitosis, aligning their chromosomes. Cohesion is then synchronously lost 5 minutes ahead of schedule, without degrading securin or cyclin B1. This premature chromatid disjunction requires the catalytic activity of separase, indicating that it is dependent on cohesin cleavage. Single chromatids then attempt to realign but the lack of tension results in unstable kinetochore-microtubule interactions and Aurora-B-dependent spindle checkpoint activation. Separase mutants that cannot bind cyclin B1 but are phosphorylated on S1126 phenocopy separase S1126A, indicating that cyclin B1 binding, rather than phosphorylation, is the key inhibitory event. Significantly, by overexpressing separase S1126A, we have simultaneously overridden the two known inhibitory mechanisms. First, by elevating separase levels above securin, securin-mediated inhibition is alleviated. Second, by preventing phosphorylation, cyclin-B1-mediated inhibition is also alleviated. Surprisingly, however, cohesion is maintained during the early stages of mitosis, indicating the existence of another mechanism that either inhibits separase or protects its substrate during early mitosis.
A Optical Random Number Generator Based on Photoevent Locations (1990)

Thesis (Ph. D.)--University of Rochester. College of Engineering and Applied Science. Institute of Optics, 1990.
Protein kinase C regulates endocytosis and recycling of E-cadherin (2002)

E-cadherin is a major component of adherens junctions in epithelial cells. We showed previously that a pool of cell surface E-cadherin is constitutively internalized and recycled back to the surface. In the present study, we investigated the potential role of protein kinase C (PKC) in regulating the trafficking of surface E-cadherin in Madin-Darby canine kidney cells. Using surface biotinylation and immunofluorescence, we found that treatment of cells with phorbol esters increased the rate of endocytosis of E-cadherin, resulting in accumulation of E-cadherin in apically localized early or recycling endosomes. The recycling of E-cadherin back to the surface was also decreased in the presence of phorbol esters. Phorbol ester-induced endocytosis of E-cadherin was blocked by specific inhibitors, implicating novel PKC isozymes, such as PKC-epsilon in this pathway. PKC activation led to changes in the actin cytoskeleton facilitating E-cadherin endocytosis. Depolymerization of actin increased endocytosis of E-cadherin, whereas the PKC-induced uptake of E-cadherin was blocked by the actin stabilizer jasplakinolide. Our findings show that PKC regulates vital steps of E-cadherin trafficking, its endocytosis, and its recycling.
Boundary conformal design of laser sintered sandwich cores and simulation of graded lattice cells using a forward homogenization approach (2020)

Laser sintering enables the production of large and complex thin-walled structures. Based on a stiffness evaluation and the manufacturability, a design approach for the additive manufacturing of large, complex, multi-curved sandwich structures was investigated through the creation of lattice structures. Therefore, unit cell and sandwich beams with lattice cell cores were modeled, simulated, manufactured, and tested. The change in lattice cell shape from a periodical structure into a non-periodical structure was shown on a demonstrator. A simulation of the sandwich beams was conducted using full solid and forward homogenization models with simple and complex elastoplastic fitted unit cells (UCs). The test specimens were printed, and three-point bending (3PB) tests were conducted. The simulation results show good agreement with the experimental data for truss-based lattice structures for periodic (7% deviation) and non-periodic lattice cell cores. To verify the design approach, a demonstrator of a segment of a racing motorcycle front fairing was designed and manufactured.
Disappearance of Stabilized Chromatic Gratings (1981)

When the image of a stationary, sinusoidal luminance grating is stabilized on the retina of a human subject, he becomes unable to detect this stimulus at contrasts that are readily visible in normal, unstabilized vision. At much higher contrasts, such stabilized gratings can still be seen over most of the normal range of spatial frequencies, although the threshold contrast may be increased by as much as 20 or 30 times. When the analogous experiment is performed with an isoluminance chromatic grating, however, there is no contrast that can restore the visibility of the stabilized grating; the threshold elevations for stabilized chromatic gratings are too great to measure. Saturated red/green gratings fade out and disappear at 100 percent contrast (even where this is 45 times the unstabilized threshold), and they do not reappear as long as stabilization is maintained. Without some kind of temporal variation of the proximal stimulus, the opponent-color pathways apparently do not respond to spatial patterns.
RNA-Seq for Enrichment and Analysis of IRF5 Transcript Expression in SLE (2013)

Polymorphisms in the interferon regulatory factor 5 (IRF5) gene have been consistently replicated and shown to confer risk for or protection from the development of systemic lupus erythematosus (SLE). IRF5 expression is significantly upregulated in SLE patients and upregulation associates with IRF5-SLE risk haplotypes. IRF5 alternative splicing has also been shown to be elevated in SLE patients. Given that human IRF5 exists as multiple alternatively spliced transcripts with distinct function(s), it is important to determine whether the IRF5 transcript profile expressed in healthy donor immune cells is different from that expressed in SLE patients. Moreover, it is not currently known whether an IRF5-SLE risk haplotype defines the profile of IRF5 transcripts expressed. Using standard molecular cloning techniques, we identified and isolated 14 new differentially spliced IRF5 transcript variants from purified monocytes of healthy donors and SLE patients to generate an IRF5 variant transcriptome. Next-generation sequencing was then used to perform in-depth and quantitative analysis of full-length IRF5 transcript expression in primary immune cells of SLE patients and healthy donors by next-generation sequencing. Evidence for additional alternatively spliced transcripts was obtained from de novo junction discovery. Data from these studies support the overall complexity of IRF5 alternative splicing in SLE. Results from next-generation sequencing correlated with cloning and gave similar abundance rankings in SLE patients thus supporting the use of this new technology for in-depth single gene transcript profiling. Results from this study provide the first proof that 1) SLE patients express an IRF5 transcript signature that is distinct from healthy donors, 2) an IRF5-SLE risk haplotype defines the top four most abundant IRF5 transcripts expressed in SLE patients, and 3) an IRF5 transcript signature enables clustering of SLE patients with the H2 risk haplotype.
Affine transform resilient image fingerprinting (2003)

Affine transformations are a well-known robustness issue in many multimedia fingerprinting systems. Since it is quite easy with modem computers to apply affine transformations to audio, image and video content, there is an obvious necessity for affine transformation resilient fingerprinting. We present a new method for affine transformation resilient fingerprints that is based upon the autocorrelation of the Radon transform, the log mapping and the Fourier transform. Besides robustness, we also address issues such as security, database search efficiency and independence with perceptually different inputs. Experimental results show that the proposed fingerprints are highly robust to affine transformations.
Application of stereo techniques to angiography: qualitative and quantitative approaches (2002)

The authors discuss the use of stereo imaging techniques as a low cost and low radiation dosage means to provide physicians with three-dimensional (3D) information for diagnosis and follow-up care of patients. Instead of using a pair of images obtained from perpendicular directions (as in the case of biplane radiography), the authors show that if a pair of small-angle stereo X-ray images are obtained, observers can vividly perceive the images in 3D during acquisition, i.e. real-time stereo visualization is possible. In addition, quantitative information such as blood flow measurements and the amount of arterial narrowing can be extracted off-line by using computational stereo algorithms. The potential advantages of using a small-angle stereo-based approach as opposed to biplane angiography are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Evidence for inverse agonism of SR141716A at human recombinant cannabinoid CB<sub>1</sub> and CB<sub>2</sub> receptors (1998)

The cannabinoid receptor antagonist SR141716A has been suggested to be an inverse agonist at CB1 receptors in some isolated intact tissues. We found that the basal incorporation of [35S]-GTPgammaS in Chinese hamster ovary cells expressing human recombinant CB1 and CB2 receptors was inhibited by SR141716A (mean pEC50s 8.26 and 6.00, respectively), whereas cannabinol (10 microM) had no significant effect at hCB1 receptors but inhibited the binding at hCB2 receptors. As cannabinol had no effect on basal [35S]-GTPmicroS binding at hCB1 at a concentration 100 fold higher than its binding affinity (K = 0.1 microM), we conclude that endogenous cannabinoid receptor agonists are not a confounding factor and suggest the actions of SR141716A at the hCB1 receptor, and the actions of SR141716A and cannabinol at the hCB2 receptor, are due to inverse agonism.
Double-Stranded RNA-Activated Protein Kinase (PKR) Fused to Green Fluorescent Protein Induces Apoptosis of Human Embryonic Kidney Cells: Possible Role in the Fas Signaling Pathway (1999)

PKR is an interferon-inducible, double-stranded (ds) RNA-activated serine/threonine protein kinase, and has been shown to play roles in viral pathogenesis, cell growth and apoptosis. We expressed PKR as a fusion protein with enhanced jellyfish green fluorescence protein (EGFP) in human embryonic kidney 293 cells to visualize the effect of PKR transfection. The EGFP-fusion construct with wild-type PKR showed both auto- and substrate-phosphorylation activities independent of dsRNA, indicating EGFP-PKR is constitutively active. The EGFP-construct with a mutant PKR with the first RNA binding domain deleted still possessed kinase activities. On the other hand, the EGFP-fusion with a catalytically inactive mutant of PKR with the substitution of K at 296 with R, which has been shown to have tumorigenic properties, did not possess kinase activities. Transfection of the constitutive active forms of EGFP-PKR constructs induced apoptosis in 293 cells without dsRNA, whereas the EGFP-fusion with the catalytically inactive mutant did not cause apoptosis but rather protected cells from Fas-induced cell death. In addition, Fas-stimulation increased endogenous PKR activities. These results constitute evidence that PKR is sufficient to induce apoptosis, and plays a role in Fas-mediated apoptosis.
Applying the theory of constraints to supply chain collaboration (2004)

Supply chain collaboration amongst independent firms often provides larger benefits from effectively satisfying end customer needs than working in isolation. However, a lack of awareness about the existence of constraints along the supply chain prevents the benefits of collaboration from being fully realised. This paper attempts to apply the theory of constraints approach to overcome difficulties in realising the potential benefits of supply chain collaboration. Specifically, it shows how the theory of constraints approach can be used to expose an inherent dilemma of collaboration and establish collaborative replenishment policy and collaborative performance metrics so that the chain members can work together to advance supply chain profitability. Several opportunities for future research are recommended.
Accurate characterization of the transmittivity of large-diameter multimode optical fibers (1987)

We describe an original technique that permits a specially accurate measurement of the variations of the transmittance of optical fibers vs the incidence of launched light. For large-diameter large-aperture fibers, which prefer light power transmission, the transmittance may be modeled using geometric optics. Taking into account the reflection losses at both ends, the core attenuation and a very weak lack of reflectivity at the core-cladding interface, good agreement is obtained between calculated variations and experimental results for the three kinds of fiber tested. The remaining difference has to be attributed to the mode coupling process which may be thus evaluated. The too high attenuation coefficient, which is attributed to the cladding to account for the losses at the core-cladding interface, characterizes in fact a thin perturbed layer where the materials contact. Thus, this method is a good means to evaluate separately the various processes contributing to the attenuation of open beams along the fibers and to determine which process should be worked on to improve the transmittance.
Tunnel Emission into Vacuum (1962)

Tunnel emission into vacuum is obtained from thin-film sandwiches of (a) aluminum-alumina-aluminum and (b) aluminum-alumina-platinum. The alumina layer is made by oxidizing an evaporated film of aluminum in air. Cesium processing, to lower the work function, results in an increase of emission of five orders of magnitude.
X-ray excited luminescence and local structures in Tb-doped Y2O3 nanocrystals (1998)

Pronounced structure in x-ray excited luminescence (XEL) has been observed in dilute Tb-doped Y2O3 (Y2O3:Tb) nanocrystals. This effect affords a means to assess different energy transfer mechanisms in the nanocrystals and also an opportunity for novel device applications. Sharp jumps and oscillations are found in the XEL output with the incident x-ray energy around the absorption edges of Y and Tb. When compared with a bulk Y2O3:Tb sample, these effects are attributed to some unique electronic and optical properties of doped nanocrystals related to quantum confinement of charge carriers, and the main features can be explained by a proposed model of multichannel energy transfer. Extended x-ray absorption fine structure techniques have also been employed to study the effect of size variation and chemical doping on the local structures in Y2O3 and Y2O3:Tb nanocrystals. The local environment surrounding Y and Tb in the nanocrystals is compared with that in the respective bulk material. The results indicate that Tb impurity atoms substitute for Y sites in bulk Y2O3, while doping in the nanocrystals is complicated by the large fraction of surface atoms and local disorder.
Three-dimensional wavelength-scale confinement in quantum dot microcavity light-emitting diodes (2004)

We introduce a microcavity light-emitting diode (LED) structure that uses submicrometer oxide aperture and a quantum dot active region to achieve strong three-dimensional confinement of both the carrier distribution and the optical field. Light–current curves show optical emission for devices as small as 400nm in diameter. Spectroscopy on electrically pumped LEDs, with apertures ranging from 2.5 down to 0.7μm, show several spectral lines corresponding to cavity modes. A strong blueshift of the resonant modes for smaller apertures demonstrates the role of the oxide aperture in confining laterally the optical wave in a volume comparable to (λ∕n)3. Due to the high quality factors and low mode volumes, the devices could be good candidates for the demonstration of the Purcell effect under electrical pumping.
Polymorphism in the Pertussis Toxin Promoter Region Affecting the DNA-Based Diagnosis of<i>Bordetella</i>Infection (2000)

The pertussis toxin (PT) promoter region is a frequently used target for DNA-based diagnosis of pertussis and parapertussis infections. The reported polymorphism in this region has also allowed discrimination of species in mixtures with several Bordetella species by their specific PCR amplicon restriction patterns. In the present study, we investigated the degree of polymorphism in order to confirm the reliability of the assay. Five different sequence types of the amplified 239- or 249-bp region were found among the 33 Bordetella pertussis, B. parapertussis, and B. bronchiseptica American Type Culture Collection reference strains and patient isolates analyzed. According to the sequences that were obtained and according to the PT promoter sequences already available in the databases, restriction enzyme analysis with TaqI, BglI, and HaeII, which gave four different patterns, can be performed to reliably identify B. pertussis, B. parapertussis, and B. bronchiseptica.
State-estimation approach to the nonstationary optical tomography problem (2003)

We propose a new numerical approach to the nonstationary optical (diffusion) tomography (OT) problem. The assumption in the method is that the absorption and/or diffusion coefficients are nonstationary in the sense that they may exhibit significant changes during the time that is needed to measure data for one traditional image frame. In the proposed method, the OT problem is formulated as a state-estimation problem. Within the state-estimation formulation, the absorption and/or diffusion coefficients are considered a stochastic process. The objective is to estimate a sequence of states for the process when the state evolution model for the process, the observation model for OT experiments, and data on the exterior boundary are given. In the proposed method, the state estimates are computed by using Kalman filtering techniques. The performance of the proposed method is evaluated on the basis of synthetic data. The simulations also illustrate that further improvements to the results in nonstationary applications can be obtained by adjustment of the measurement protocol.
Micrometastasis in breast cancer and other solid tumors. (2005)

Hematogenous distant metastasis is the leading cause of cancer-related death in breast cancer and other solid tumors. By applying sensitive immunocytochemical and molecular assays, disseminated tumor cells (DTC) in bone marrow (BM) can be detected in 20-40% of cancer patients without any clinical or even histopathological signs of metastasis and the presence of these DTC at primary diagnosis predicts the subsequent occurrence of overt metastases in bone and other organs. cDNA-microarray analysis on primary breast carcinomas from patients with and without tumor cells in BM revealed a predominant downregulation of potential metastasis-suppressor genes in BM-positive tumors. Thus, dissemination of tumor cells appears to be an early process associated with a specific molecular signature of the primary tumor.
Long-term bias temperature reliability of P/sup +/ polysilicon gated FET devices (1995)

An instability was found to be associated with +BT stress for P/sup +/ poly-gated NMOSFETs (PNMOS) and PMOSFETs (PPMOS), but not with the N/sup +/ poly-gated devices (NNMOS and NPMOS). The instability with the P/sup +/ poly-gated devices, which is a decrease in threshold voltage (V/sub t/) and an increase in interface state density (D/sub it/), was significantly reduced following N/sub 2/ annealing at 400/spl deg/C. It is shown that adequate reliability for P/sup +/ poly-gated devices can be achieved for VLSI technologies.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Polymer Gels that Memorize Structures of Mesoscopically Sized Templates. Dynamic and Optical Nature of Periodic Ordered Mesoporous Chemical Gels (2002)

A periodically ordered interconnecting porous structure could be imprinted in thermosensitive hydrogels by using silica colloidal crystals as mesoscopically sized templates. The interconnecting porous structure provides a fast response to the change in temperature for reversible swelling and shrinking of the hydrogels, while the periodically ordered mesoscopical structure endows a structural color to the gel. The structural color imprinted in the gel is quickly synchronized with the change in the volume of the gels. The newly invented mesoporous hydrogels possess optical properties as temperature sensitive smart gels.
Reconciling Differences in Rating the Quality of Health Care Administration Journals Between Program Chairs?? Perceptions and Citation Counts: Cutting Edge, Historical Seniority, Mainstream, Measurement Error, or Political Correctness (1994)

*From Daviess County Hospital, Washington, Indiana †From Regenstrief Institute for Health Care, and the Department of Medicine, School of Medicine, Indiana University, Indianapolis
Genomic multiple sequence alignments: refinement using a genetic algorithm (2005)

We have implemented a genetic algorithm in parallel mode to optimize multiple genomic sequence alignments initially generated by various alignment tools. Benchmarking experiments showed that the refinement algorithm improved genomic sequence alignments within a reasonable period of time.