gebhart/patrepeval huggingface dataset:
Immunologic detection of endothelial cells in human whole blood (1991)

In this report we show that human endothelial cells (EC) can be detected in circulating blood by means of the EC-specific monoclonal antibody (MoAb) designated as CLB-HEC 19 and expressed quantitatively as number of cells per milliliter of whole blood. We first developed a method that was able to recover cultured human EC added to whole blood by Percoll density gradient centrifugation. The final recovery of the EC was 91.6% (SE = 0.65%). The EC were identified in the gradient subfractions by indirect immunofluorescence with the MoAb CLB-HEC 19. This method was then applied to the separation and characterization of EC or EC remnants from the whole arterial and venous blood taken from two groups of patients subjected to heart catheterization. Firstly, a preliminary blood screening of random samples was performed in a group of eight patients (group I) using a scoring evaluation for the presence of EC and the results were expressed as positivity index. Secondly, the complete blood screening of a group of ten patients (group II) was performed for the detection of immunofluorescent cells and the results were expressed as number of EC per milliliter of whole blood. Our results show in both group I and II a significant presence of EC in the blood after catheterization compared with their basal values. The minimal detectable concentration of EC was 0.06 cells/mL (SE = 0.057) of whole blood. We consider this technique as a suitable clinical test for the detection of EC injury in cardiovascular pathology.
Release studies of benzalkonium chloride from hydrogel in a freshwater environment (2003)

The use of underwater optical sensors to monitor pollution and climate change processes has led to the development of robust instruments able to be deployed in lakes and seas for months at a time. However, despite this improvement in their durability they are subject to biofouling on their optical ports resulting in erroneous readings. The use of hydrogel coatings containing the cationic surfactant benzalkonium chloride (BAC) has been shown to prevent the development of biofouling for up to 12 weeks in the marine environment. In this study the use of hydrogel coatings in the freshwater environment was less successful with fouling visible at 2 weeks. In both field and laboratory studies a rapid initial loss of BAC from the hydrogel film was observed. The loss is a combination of diffusive and mass flow but the period from 12 to 50 h appeared to fit to diffusion kinetics and a diffusion coefficient of 7.3 x 10(-8) cm2 s(-1) (13 degrees C) was calculated, an order of 10 times greater than that found in seawater. Subsequently the rate of loss of the residual BAC, for which a diffusion coefficient of 5.7 x 10(-10) cm2 s(-1) (15 degrees C) was measured, was too low to prevent the early stages of biofouling.
Gate dependent photo-responses of carbon nanotube field effect phototransistors (2012)

Gate dependent photoconductivity of carbon nanotube (CNT) field effect phototransistors (FEPs) was systematically investigated in this study. The photo-response comparisons of CNT FEPs with symmetric and asymmetric metal structures connecting to the same CNT revealed that the gate effect contributed to a sensitivity improvement with a lower dark current, a higher photocurrent, and an enhanced photovoltage. A functionalized asymmetric FEP, fabricated by partially doping the CNT utilizing a polyethylene imine (PEI) polymer, verified that FEPs delivered a better performance by using asymmetric structures. A multi-gate FEP, with three pairs of side-gates that can electrostatically dope different sections of a CNT independently, was fabricated to examine the gate structure dependent photo-responses. Experimental measurements showed an unconventional photocurrent improvement that was weakly dependent on the gate location, which was attributed to the unique charge distribution of one-dimensional semiconductors. © 2012 IOP Publishing Ltd.
Characterization of spliced leader genes of <i>Trypanosoma</i> (<i>Megatrypanum</i>) <i>theileri</i>: phylogeographical analysis of Brazilian isolates from cattle supports spatial clustering of genotypes and parity with ribosomal markers (2009)

Trypanosoma (Megatrypanum) theileri from cattle and trypanosomes of other artiodactyls form a clade of closely related species in analyses using ribosomal sequences. Analysis of polymorphic sequences of a larger number of trypanosomes from broader geographical origins is required to evaluate the clustering of isolates as suggested by previous studies. Here, we determined the sequences of the spliced leader (SL) genes of 21 isolates from cattle and 2 from water buffalo from distant regions of Brazil. Analysis of SL gene repeats revealed that the 5S rRNA gene is inserted within the intergenic region. Phylogeographical patterns inferred using SL sequences showed at least 5 major genotypes of T. theileri distributed in 2 strongly divergent lineages. Lineage TthI comprises genotypes IA and IB from buffalo and cattle, respectively, from the Southeast and Central regions, whereas genotype IC is restricted to cattle from the Southern region. Lineage TthII includes cattle genotypes IIA, which is restricted to the North and Northeast, and IIB, found in the Centre, West, North and Northeast. PCR-RFLP of SL genes revealed valuable markers for genotyping T. theileri. The results of this study emphasize the genetic complexity and corroborate the geographical structuring of T. theileri genotypes found in cattle.
Simulation of CO&lt;sub&gt;2&lt;/sub&gt; and H&lt;sub&gt;2&lt;/sub&gt;S Removal Using Methanol in Hollow Fiber Membrane Gas Absorber (HFMGA) (2012)

Application of methanol solvent for physical absorption of CO2 and H2S from CO2/H2S/CH4 mixture in gas–liquid hollow fiber membrane gas absorber (HFMGA) was investigated. A computational mass transfer (CMT) model for simulation of HFMGA in the case of simultaneous separation of CO2 and H2S was developed. The membrane gas absorber model explicitly calculates for the rates of mass transfer through the membrane and components concentration profiles. Due to the lack of experimental data in the literature, the model was validated using available individual components’ water absorption data. The numerical predictions were in good agreement with the experimental data. The effects of operating conditions such as liquid velocity, gas velocity, temperature and pressure were analyzed. It is shown that methanol solvent can successfully be used for CO2 and H2S removal in membrane gas absorber. Also it is found that the concentration distribution of CO2 and H2S in the gas phase along the fiber length obeys plug flow model whereas in the methanol absorbent deeply affected by the interface concentration, absorbent velocity and diffusivity. In addition, it is shown that application of membrane gas absorber using methanol absorbents for H2S removal and at higher flow rate is more efficient. Moreover, at operating pressures above 10 atm even at low absorbent rate, H2S concentration depletion is relatively complete while at 1 atm this value is about 30%. This means that removal efficiency decreases with an increase in temperature and it is more important especially for H2S.
An investigation of Cutimed ® Sorbact ® as an antimicrobial alternative in wound management (2008)

Background: Antimicrobial dressings play a major role in the effective management of both acute and chronic wounds. The study featured in this article investigates whether the use of a new dressing, which incorporates the principle of hydrophobic interaction, offers an effective form of antimicrobial management in chronic wounds. Aims: This study aimed to assess the efficacy of Cutimed® Sorbact® (BSN medical Ltd, Hull) in the management of chronic wounds. Methods: A 116-patient multi-centre European study was used. Results: The study results demonstrate that 81% of patients with an infected wound received effective treatment using the new dressing. Twenty-one per cent of patients’ wounds healed during the study and a further 72% showed an improvement in wound healing. Reductions in wound pain were reported and both patients and healthcare professionals reported the dressing as being easy to use. A range of secondary dressings were used during the study and no compatibility issues were reported. The principle of hydrophobicity in dressings offers an alternative to the use of established antimicrobial products. Conclusions: The adaptability of a bacterial binding product offers a great deal to healthcare professionals involved in the management of wounds and should be considered a welcome development Conflict of interest: This study was performed in response to the application and request of the Academy for Certified Wound Management and was subsequently sponsored by BSN medical GmbH (Hamburg)
Cyclocarbopalladation of Alkynes:  A Stereoselective Method for Preparing Dibenzoxapine Containing Tetrasubstituted Exocyclic Alkenes (2006)

[reaction: see text] A palladium-catalyzed cascade carbometalation-cross coupling of alkyne route was developed for the preparation of tetrasubstituted exocyclic alkenes with high stereo- and regiocontrol. The effectiveness of this novel methodology was demonstrated by the synthesis of a number of dibenzoxapines in sufficient quantities to support their further development.
Role of Intracellular Proteins in the Regulation of Calcium Action and Transmitter Release During Exocytosis (2015)

A central problem in cellular neurobiology is how the process of exocytosis of transmitters and hormones is regulated at the molecular level. Calcium is important in the process and may act by initiating the formation of fusion complexes of secretory granules to each other and to plasma membranes. We have discovered and isolated a new 47,000 MW protein (synexin) from adrenal medulla tissue that fuses chromaffin granule membranes only in the presence of calcium. Synexin activity was detected in a number of secretory tissues including human platelets and bovine brain, and the synexin molecule was found by immunofluorescent cytochemistry to be localized to the cytoplasm of chromaffin cells. Purified synexin molecules were found to self-associate in the presence of Ca++ to form paracrystalline arrays of 50 X 150 A rods, and the association was dependent on [Ca++] in an identical fashion to the Ca++ dependence of granule membrane fusion. On the basis of these data we suggest that synexin may be the intracellular receptor for calcium during exocytosis. However, the actual release event of 'fission' of the secretory vesicle-plasma membrane complex did not appear to be related to synexin action, and we have considered the hypothesis that the chemiosmotic mechanism for ATP, Cl--dependent chromaffin granule lysis might provide the necessary localized force. We have now shown that the granule model successfully predicts the secretory properties of human platelets, bovine parathyroid cells, and bovine chromaffin cells. Like the granule lysis system, secretion from these cells required specific anions in the medium was inhibited by anion transport blocking drugs and proton ionophores, and was suppressed by elevated osmotic strength. We suggest that secretory granules fused to plasma membranes in secreting cells, perhaps by synexin, may experience net solute uptake and subsequently undergo local, outwardly directed osmotic lysis, or exocytosis.
Solid-state microwave power source (1959)

IT IS POSSIBLE to generate microwave power using a transistorized oscillator and an amplifier running at a few hundred megacycles, together with a diode harmonic multiplier. The aim of this paper is to show the most favorable mode of operation of a diode as well as the best device parameters for optimum harmonic generation.
A hybrid herpesvirus infectious vector based on Epstein-Barr virus and herpes simplex virus type 1 for gene transfer into human cells in vitro and in vivo. (2020)

We have developed a miniviral vector, pH300, based on the human herpesviruses 1 and 4, herpes simplex virus type 1 (HSV-1), and Epstein-Barr virus (EBV), carrying EBV sequences for plasmid episomal maintenance and HSV-1 sequences for amplification and packaging in multimeric form into HSV-1 capsids in the presence of a helper virus and helper cell line. A reporter gene, the bacterial lacZ gene, which expressed beta-galactosidase, was inserted into the multiple cloning site of pH300 to make pH300-lac. The packaged pH300-lac DNA was very efficient in infecting human cells in tissue culture. The pH300-lac miniviral stock was used to infect in vitro various human cell types derived from breast cancer, lung cancer, and liver cancer. Up to 95% of cells were infected and expressed beta-galactosidase activity after exposure to viral stock at a multiplicity of infection of 3. There was essentially no apparent cytotoxicity after infection of cultured cells in vitro. To test in vivo gene delivery, human liver tumor cells preimplanted subcutaneously in nude mice and injected in situ with pH300-lac showed high efficiency of ectopic gene expression. The pH300 miniviral vector is a simple and effective gene transfer system which shows potential for gene therapy of cancer and inherited diseases.
A 130 MHz 8-Bit CMOS Video DAC for HDTV Applications (1990)

A 130 MHz CMOS video DAC with a current output for HDTV applications will be described. In order to achieve monotonicity and a high speed performance,a current cell matrix configuration and a parallel decoding circuit with one stage latches have been used. P channel devices used as current sources insure a low noise level and a ground referenced voltage output in double adaptation load (38 ohms / 20 pF). The experimental results have shown that the maximum conversion rate is 130 MHz and the integral and differential linearity errors are less than 0.5 LSB. The maximum glitch energy is 50 pS.V. The DAC has been developed in a 1 ?m digital/analog CMOS technology. It dissipates 150 mW at a 130 MHz conversion rate.
Use of a disease severity index for evaluation of healthcare costs and management of comorbidities of patients with diabetes mellitus. (2002)

A diabetes mellitus-specific risk stratification system related to required care intensity can be used to identify patients with high medical costs and can enable care providers to select patients for case management and triage into specific care programs.
Matrix metalloproteinase-9 expression in bladder washes from bladder cancer patients predicts pathological stage and grade. (1998)

The matrix metalloproteinases (MMPs), in particular the gelatinases (MMP-2 and MMP-9) have been associated with tumor cell invasion and metastasis in many human cancers. Here we examined the expression of proMMP-2 (gelatinase A) and proMMP-9 (gelatinase B) proteins in the cellular component of bladder washes obtained from 65 patients. Twenty-six patients had active bladder cancer, 24 had a history of bladder cancer but no evidence of active disease at the time of cystoscopy (recurrence-free), and 15 patients had lesions other than bladder cancer (controls). The results were correlated with the cytological findings of the bladder wash and the histopathological results of the tumor resection when performed. In patients with active transitional cell carcinoma of the bladder, 71 and 38% had expression and overexpression of the latent form of MMP-9 (proMMP-9), respectively. In contrast, neither latent nor active MMP-2 could be detected in any of the samples examined, regardless of tumor status. Overexpression of proMMP-9 correlated with higher grade (P = 0.003) and pathological stage (P = 0.04) of disease in the active bladder cancer group. No significant gelatinase expression was detected in the recurrence-free and control cases. Compared with urine cytology, proMMP-9 expression had an overall higher sensitivity for bladder cancer identification (71 versus 54%, P = 0.11). Detection of proMMP-9 in bladder washes may be a novel approach for the identification of patients with more aggressive forms of bladder cancer.
Introduction (1985)

This is a policy-oriented book on international finance. Rather than attempting to be completely comprehensive, it sets out to provide a succinct treatment of some of the more important issues that have come to dominate discussions of international financial reform. Given the increasingly frequent calls that have been made for a new Bretton Woods conference to reform existing international financial arrangements, it seems appropriate to examine the items that would be on the agenda for such a conference, and this is the book's principal objective.
Resistance of<i>Escherichia coli</i>to Penicillins IX. Genetics and Physiology of Class II Ampicillin-Resistant Mutants That Are Galactose Negative or Sensitive to Bacteriophage C21, or Both (1971)

Ampicillin-resistant mutants of class II are determined by a doubling of chromosomally and episomally mediated ampicillin resistance on agar plates. Several mutants were isolated from a female as well as from an Hfr strain. The mutants differed from each other in various properties such as response to colicin E2 and sodium cholate, response to the phages T4 and C21, and fermentation of galactose. By conjugation and transduction experiments, it was shown that mutations in at least four loci gave the class II phenotype. The mutations were found to be in the galU gene, the ctr gene, and two new genes close to mtl denoted lpsA and lpsB. The carbohydrate compositions of the lipopolysaccharides of the mutants were investigated and found to be changed compared to the parent strains. GalU mutants lacked rhamnose and galactose and had 11% glucose compared to the parent strain. The lpsA mutant also lacked rhamnose and had only traces of galactose and 58% glucose, whereas the lpsB mutant contained 14% rhamnose, traces of galactose, and 81% glucose compared to the parent strain.
Determination of Calcium Sulfoaluminate in Cement Paste by Tracer Technique (1960)

First Name is required invalid characters Last Name is required invalid characters Email Address is required Invalid Email Address Invalid Email Address
Image sampling in static telepathology for frozen section diagnosis. (1999)

The results suggest that in routine frozen section diagnosis an inexperienced pathologist can sample images sufficiently well to permit remote diagnosis. However, as expected, the internet is too unreliable for such a time dependent task. An improvement in the system would involve integrated real time features, so that there could be interaction between the two pathologists.
Synthesis and Physical Properties of Triacylglycerol Oligomers: Examining the Physical Functionality Potential of Self-Metathesized Highly Unsaturated Vegetable Oils (2013)

Seven model oligomers (from dimer to octamer) of the triacylglycerol (TAG) triolein were synthesized from oleic acid and fully characterized by 1H NMR, 13C NMR, mass spectroscopy, and gel permeation chromatography (GPC). The thermal stability of the oligomers as determined by TGA was excellent, with degradation beginning at 342 °C for the most thermally labile samples. The samples all presented glass transitions at low temperatures, with Tg continuously shifting to higher temperatures with increasing numbers of monomers. The crystallization and melting behavior scaled with molecular size and relative number of double bonds in the trans- configuration. Flow behavior was investigated over a large range of temperatures (−10 to 110 °C), and application of the Herschel–Bulkey model to shear stress versus shear rate data evidenced a flow behavior dependent on molecular size and temperature. The oligomers presented a thinning to Newtonian flow transition temperature proportional to molecular size. The viscosity versus temperature data, fitted with a generalized van Velzen equation, suggested that it is the competition between the trans- character and size of the molecules which determines the rheology of these molecules. Overall, all the investigated properties plateaued at the hexamer, suggesting that no further marginal utility can be obtained with larger oligomers.
An MBSE modeling approach to efficiently address complex systems and scalability (2018)

Abstract For decades Airbus has largely used modeling and simulation techniques all along the development cycle. Nowadays, the use of systemic approaches, associated with proper modeling tools and technics, provides efficient solutions to leverage associated benefits while addressing the increasing systems complexity. This paper highlights some of these modeling principles, providing ways to improve, ease and make design activities more efficient, and providing solutions to better master the overall design complexity. The paper illustrates how to combine them, thanks to a given model structure, based on a deployment case performed within Airbus. Such an implementation requires significant customization efforts, raising risks to well support its deployment in extended enterprise, with other partners. Out of specificities linked to features of used modeling tools, the goal of this paper is to share with the MBSE community some root principles and needs, in order to have them better involved into future tools and standards.
SANITARY CONDITIONS IN PERU (1921)

SANITARY CONDITIONS IN PERU Henry Hanson CopyRight https://doi.org/10.2105/AJPH.11.1.13 Published Online: August 30, 2011
Anaerobic Bacteria in Human Disease. (1977)

Anearobic bacteria in human disease , Anearobic bacteria in human disease , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
A designed fluid cracking catalyst with vanadium tolerance (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA designed fluid cracking catalyst with vanadium toleranceC. A. Altomare, G. S. Koermer, P. F. Schubert, S. L. Suib, and W. S. WillisCite this: Chem. Mater. 1989, 1, 4, 459–463Publication Date (Print):July 1, 1989Publication History Published online1 May 2002Published inissue 1 July 1989https://pubs.acs.org/doi/10.1021/cm00004a014https://doi.org/10.1021/cm00004a014research-articleACS PublicationsRequest reuse permissionsArticle Views116Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts