gebhart/patrepeval huggingface dataset:
Novel short transcripts of hepatitis B virus X gene derived from intragenic promoter. (1994)

The hepatitis B virus X gene encodes a transcriptional trans-activator that appears essential for viral replication in the infected liver. It has been shown by others that at least two X protein products, initiating at different in-frame ATG codons within the X open reading frame, are necessary for full trans-activating function. Yet, previous studies have demonstrated only a full-length X gene transcript that extends 5' of the first ATG codon in the X open reading frame. In this communication, we present evidence for the existence of shorter X gene transcripts, whose heterogeneous 5' ends straddle the second ATG codon of the X open reading frame. These transcripts are not degradation products of the full-length X gene transcripts but rather arise from an independent promoter. Furthermore, immunofluorescence and functional analyses reveal that active X proteins are translated from these transcripts. Therefore, there appears to be a previously undescribed promoter embedded within the X open reading frame that can give rise to functionally important transcripts.
Organometallic Compounds with Cage Structures: 1-Adamantyl Lithium (1978)

Abstract Although the formation mechanism of organolithium compounds (R-Li) is relatively little known,1 the presence of duplication products R-R seems to indicate that it is of a radical nature.
Monolithic, Ultra-Thin GaInP/GaAs/GaInAs Tandem Solar Cells (2006)

We present here a new approach to tandem cell design that offers near-optimum subcell bandgaps, as well as other special advantages related to cell fabrication, operation, and cost reduction. Monolithic, ultra-thin GaInP/GaAs/GaInAs triple-bandgap tandem solar cells use this new approach, which involves inverted epitaxial growth, handle mounting, and parent substrate removal. The optimal ~1-eV bottom subcell in the tandem affords an -300 mV increase in the tandem voltage output when compared to conventional Ge-based, triple-junction tandem cells, leading to a potential relative performance improvement of 10-12% over the current state of the art. Recent performance results and advanced design options are discussed
Potentiation of the cardiovascular effects of nitroglycerin by N-acetylcysteine. (1983)

The biochemical basis of the mechanism of vasodilatation by nitroglycerin (NTG) has not been previously investigated in man. However, evidence from in vitro studies suggests that NTG induces activation of guanylate cyclase via a series of enzymatic reactions that are modulated by the availability of sulfhydryl groups. Cysteine appears to be particularly effective in potentiating guanylate cyclase activation by NTG. To determine whether hemodynamic responsiveness to NTG in man might be modulated by sulfhydryl availability, concentration-response curves for effects of intravenously infused NTG on mean arterial pressure (MAP) and mean pulmonary capillary wedge pressure (PCW) were obtained in 10 patients undergoing cardiac catheterization for investigation of chest pain. NTG infusion was repeated 10 min after the intravenous infusion of 100 mg/kg of the cysteine source N-acetylcysteine (NAC). NAC induced no significant hemodynamic effect, but after NAC infusion there was a significant reduction both in the NTG infusion rate associated with a 10% fall from control values in MAP (25.8 +/- 8.3 to 9.3 +/- 2.7 micrograms/min; p less than .01) and in the infusion rate inducing a 30% reduction in PCW (13.6 +/- 4.6 to 4.2 +/- 1.6 micrograms/min; p less than .02). In a control group of five patients who received no NAC, there was no significant change in responsiveness to NTG between infusions. It is concluded that NAC potentiates the vasodilator effects of NTG in man. This suggests that sulfhydryl availability and/or redox state may be determinants of in vivo responsiveness to NTG.
Pyrolysis as an Analytical Tool (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPyrolysis as an Analytical ToolR. N. Rogers, S. K. Yasuda, and John. ZinnCite this: Anal. Chem. 1960, 32, 6, 672–678Publication Date (Print):May 1, 1960Publication History Published online1 May 2002Published inissue 1 May 1960https://pubs.acs.org/doi/10.1021/ac60162a031https://doi.org/10.1021/ac60162a031research-articleACS PublicationsRequest reuse permissionsArticle Views101Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[Quality of life of a hospitalized vagrant with type 2 diabetic mellitus: applying Parse practice methodology]. (2005)

Diabetic patients who don't control their disease well often develop complications. Their quality of life can then be substantially affected. Lacking social support a vagrant who develops such complications will find it particularly hard to maintain a good quality of life. The application of Parse practice methodology with supplementary social-support theory can help a vagrant diabetic patient to meet his needs and improves his quality of life. The purpose of paper is to provide nurses with a point of reference in relation to the care of such patients.
Mammalian cell metabolomics: Experimental design and sample preparation (2013)

Metabolomics represents the global assessment of metabolites in a biological sample and reports the closest information to the phenotype of the biological system under study. Mammalian cell metabolomics has emerged as a promising tool with potential applications in many biotechnology and research areas. Metabolomics workflow includes experimental design, sampling, sample processing, metabolite analysis, and data processing. Given their influence on metabolite content and biological interpretation of data, a good experimental design and the appropriate choice of a sample processing method are prerequisites for success in any metabolomic study. The use of mammalian cells in the metabolomics field involves harder sample processing methods, including metabolism quenching and metabolite extraction, as compared to the use of body fluids, although such critical issues are frequently overlooked. This review aims to overview the common experimental procedures used in mammalian cell metabolomics based on mass spectrometry, by placing special emphasis on discussing sample preparation approaches, although other aspects, such as cell metabolomics applications, culture systems, cellular models, analytical platforms, and data analysis, are also briefly covered. This review intends to be a helpful tool to assist researchers in addressing decisions when planning a metabolomics study involving the use of mammalian cells.
Inhibition of postsurgical adhesions in a standardized rabbit model: II. Intraperitoneal treatment with heparin. (1992)

The formation of adhesions after peritoneal trauma is thought to result from the deposition of fibrin and its subsequent organization by fibroblast ingrowth and, in some cases, neovascularization and reepithelialization. Since heparin is an effective anticoagulant, and clotting is a major contributor to postsurgical fibrin deposition, we studied the effects of heparin delivered locally into the peritoneal cavity at the site of the injury on the subsequent formation of postsurgical adhesions. A flap of parietal peritoneum (approximately 1 mm thick) was dissected from the right lateral peritoneal sidewall of New Zealand white female rabbits. The serosal surface of the adjacent small bowel was abraded with a scalpel to produce punctuate bleeding. This area between the excised parietal peritoneum and adjacent small bowel serosa was then used for evaluating the efficacy of heparin for adhesion prevention. Alzet minipumps containing phosphate-buffered saline with varying doses of heparin were sewn into the right dorsal subcutaneous space with Vicryl sutures. A polyethylene catheter tip leading from the pump into the peritoneal cavity was placed 2-3 mm over the injury test site. Within seven days after the implantation of the pumps, the rabbits were killed and the extent of adhesions determined, according to a qualitative grading system. The threshold dosage at which significantly beneficial antiadhesion properties were obtained was 1.5 x 10(-2) U/hour or 7.5 x 10(-3) U/kg/day. Effective adhesion prevention occurred with only two days of treatment beginning the day of surgery. At this dose, no postsurgical bleeding occurred. These findings suggest that local intraperitoneal administration of low-dose heparin throughout the immediate postoperative interval may result in adhesion-free healing.
Patterns of Antibody Binding to Aquaporin-4 Isoforms in Neuromyelitis Optica (2010)

Our results confirm M-23 AQP4-IgG Abs as reliable biomarkers in patients with NMO and high risk syndromes. M-1 and M-23 AQP4-IgG Abs are significantly associated with a higher number of relapses and longer disease duration.
Differences observed in the graft compatibility between some cultivars of Muscadine grape (Vitis rotundifolia Michx.) and European grape (Vitis vinifera L. cv. Cabernet Sauvignon). (2016)

Well-known graft incompatibility between the Muscadine grape (Vitis rotundifolia MICHX.) and European grape (V. vinifera L.) is easily overcome with the green grafting method. But the results depend greatly on the genotypes of the Muscadine grape used. Typical symptoms of incompatibility appear after one year's growth when V. vinifera cv. Cabernet Sauvignon is grafted on Muscadine varieties like Carlos and Male. Other Muscadine varieties like Yuga and Noble do not show any symptom. These observations rule out the hypothesis that graft incompatibility between Muscadine grape and European grape was due to the existing structural and anatomical differences between these two species. Macroscopical and microscopical examination of the graft union lets us think that the observed symptoms can be related to a translocated incompatibility. The role of a hypothetical grafttransmissible factor is under investigation. Différences observées dans la compatibilité au greffage de quelques variétés de Muscadine (Vitis rotundifolia Michx.) avec Vitis vinifera L. cv. Cabernet Sauvignon L'incompatibilité au greffage reconnue depuis longtemps entre la Muscadine (Vitis rotundifolia MICHX.) et la vigne européenne (V. vinifera L.) est facilement surmontable par la méthode de la greffe en vert. Mais les résultats varient en fonction de la variété de Muscadine utilisée. Des symptômes typiques d'incompatibilité se manifestent tardivement lorsque V. vinifera cv. Cabernet Sauvignon est greffé sur les variétés de Muscadine Carlos ou Male. A l'opposé, les variétés Yuga et Noble ne manifestent aucun symptôme. Ces observations permettent d'écarter l'hypothèse selon laquelle l 'incompatibilité au greffage entre V. rotundifolia et V. vinifera serait due aux différences anatomiques et structurales qui existent entre ces deux espèces. L'examen macroscopique et microscopique du point de greffe laisse penser que les symptômes observés peuvent être rattachés à une incompatibilité «de translocation». L'hypothèse d'un facteur viral responsable des différences observées d'incompatibilité au greffage est actuellement en cours de vérification.
MMOGs as Learning Environments: An Ecological Journey into "Quest Atlantis" and "The Sims Online" (2006)

Yes, video games are mainly for play and fun. But video games are educative as well as interesting and engaging—something that we all hope that more classrooms could be. Many of today's students spend more time playing video games than they do watching television, reading books, or watching films. Massively multiplayer online games (MMOGs)—long and surprisingly complex gaming environments that normally require over forty hours to get beyond novice levels (Squire 2004)—represent the latest development in the history of video game technology (Exhibit 1). Success in a MMOG requires developing new literacies, understanding intricate and intersecting rule sets, thinking creatively within constraints, collaborating with other participants towards shared goals, and perhaps most importantly, taking on new identities as players (via their avatars) inhabit game spaces (Gee 2003). Such properties offer significant potential for educational contexts, as indicated by the emergence of MMOGs specifically designed to enable student interactions and centered on instructional topics (e.g., Quest Atlantis, AquaMoose 3D, and RiverCity).
IQ™ Technology: Development of a Universal, Homogeneous Method for High-Throughput Screening of Kinase and Phosphatase Activity (2003)

IQ™ Technology is a homogeneous, universal detection platform designed specifically for high-throughput screening of kinases and phosphatases. The technology has been tested in 96- to 384- to 1536-well microplate formats and is ideally suited for automated drug discovery screening systems. IQ™ Technology is a direct, non-competitive assay format that does not require antibodies or radioactive reagents to measure phosphorylation state. Kinase or phosphatase activity is quantitated by direct measurement of the phosphorylation state of fluorophore-labeled peptide substrates. Phosphorylation is measured by the change in fluorescence intensity that occurs when a proprietary iron-containing compound binds specifically to phosphoryl groups on peptides. This change in observed fluorescence is proportional to the extent of phosphorylation of the fluorophore-labeled peptide, because the iron-containing compound quenches the fluorescent emission exclusively on the phosphorylated form of the fluorophore-labeled pepti...
COMPUTATIONAL DNA SEQUENCE ANALYSIS (1994)

This paper reviews several new developments in computer and statistical analysis of DNA and protein sequences. We present criteria and describe means for assessing and interpreting genomic inhomogeneities within and between sequences. These include: (a) characterizations of short oligonucleotide biases and general compositional tendencies; (b) molecular evolutionary reconstructions based on dinucleotide relative abundance distance measures and partial orderings; and (c) the application of r-scan statistics, quantile distributions, and score-based analyses to identify clustering, overdispersion, and excessive evenness in the distribution of a marker array along a sequence. These apply, for example, to restriction sites, microsatellite runs, regulatory motifs, and nucleosome placements. Furthermore, (d) the definition and determination of rare and frequent oligonucleotides and peptides provides another perspective on sequence heterogeneity, and (e) score methods are also applied in exon and gene locations. Most of the ideas and methods are illustrated with respect to bacteriophage genomes, to megabase amounts of several eukaryotic sequences, to a diverse collection of bacterial sets, to mitochondrial chromosomes, and to a broad assembly of viral genomes.
Ionic Strength and Composition Affect the Mobility of Surface-Modified Fe<sup>0</sup> Nanoparticles in Water-Saturated Sand Columns (2008)

The surfaces of nanoscale zerovalent iron (NZVI) used for groundwater remediation must be modified to be mobile in the subsurface for emplacement. Adsorbed polymers and surfactants can electrostatically, sterically, or electrosterically stabilize nanoparticle suspensions in water, but their efficacy will depend on groundwater ionic strength and cation type as well as physical and chemical heterogeneities of the aquifer material. Here, the effect of ionic strength and cation type on the mobility of bare, polymer-, and surfactant-modified NZVI is evaluated in water-saturated sand columns at low particle concentrations where filtration theory is applicable. NZVI surface modifiers include a high molecular weight (MW) (125 kg/mol) poly(methacrylic acid)-b-(methyl methacrylate)-b-(styrene sulfonate) triblock copolymer (PMAA-PMMA-PSS), polyaspartate which is a low MW (2-3 kg/mol) biopolymer, and the surfactant sodium dodecyl benzene sulfonate (SDBS, MW = 348.5 g/mol). Bare NZVI with an apparent zeta-potential of -30 +/- 3 mV was immobile. Polyaspartate-modified nanoiron (MRNIP) with an apparent zeta-potential of -39 +/- 1 mV was mobile at low ionic strengths (< 40 mM for Na+ and < 0.5 mM for Ca2+), and had a critical deposition concentration (CDC) of approximately 770 mM Na+ and approximately 4 mM for Ca2+. SDBS-modified NZVI with a similar apparent zeta-potential (-38.3 +/- 0.9 mV) showed similar behavior (CDC approximately 350 mM for Na+ and approximately 3.5 mM for Ca2+). Triblock copolymer-modified NZVI had the highest apparent zeta-potential (-50 +/- 1.2 mV), the greatest mobility in porous media, and a CDC of approximately 4 M for Na+ and approximately 100s of mM for Ca2+. The high mobility and CDC is attributed to the electrosteric stabilization afforded by the triblock copolymer but not the other modifiers which provide primarily electrostatic stabilization. Thus, electrosteric stabilization provides the best resistance to changing electrolyte conditions likely to be encountered in real groundwater aquifers, and may provide transport distances of 10s to 100s of meters in unconsolidated sandy aquifers at injection velocities used for emplacement.
new strategy to guarantee retirement income using TIPS and longevity insurance (2009)

Retirees investing their savings in stocks and bonds face the risk of financial ruin even when withdrawing as little as 4% annually. This paper proposes a new investment strategy using Treasury Inflation Protected Securities and longevity insurance that would guarantee real annual withdrawal rates in excess of 5% without any risk of financial ruin. The strategy can be implemented at minimal cost by retirees and their financial advisers. Institutional providers can use this strategy to offer products that would provide inflation adjusted lifetime incomes and allow retirees to retain control over most of their savings in retirement.
The inhibition of acetylcholine synthesis in brain by a hemicholinium (1961)

Research Article| November 01 1961 The inhibition of acetylcholine synthesis in brain by a hemicholinium JE GARDINER JE GARDINER Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1961) 81 (2): 297–303. https://doi.org/10.1042/bj0810297 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation JE GARDINER; The inhibition of acetylcholine synthesis in brain by a hemicholinium. Biochem J 1 November 1961; 81 (2): 297–303. doi: https://doi.org/10.1042/bj0810297 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1961 The Biochemical Society1961 Article PDF first page preview Close Modal You do not currently have access to this content.
The Region with a Future, The Gulf and Lower St. Lawrence, par THE LOWER ST. LAWRENCE AND GULF DEVELOPMENT ASSOCIATION. Une brochure, 83 pages. Montréal, 1959 (1960)

The Region with a Future, The Gulf and Lower St. Lawrence, par THE LOWER ST. LAWRENCE AND GULF DEVELOPMENT ASSOCIATION. Une brochure, 83 pages. Montréal, 1959. Un article de la revue L'Actualité économique (Volume 36, numéro 1, avril–juin 1960, p. 5-196) diffusée par la plateforme Érudit.
Designing time partitions for real-time hypervisor with sufficient temporal independence (2015)

Virtualization techniques for embedded real-time systems, as known from the Integrated Modular Avionics (IMA) architecture of the ARINC653 standard, typically employ a TDMA scheduling to achieve temporal isolation among different virtualized partitions. Due to the fixed TDMA schedule, the worst case interrupt response times are significantly increased. An already proposed technique to mitigate this problem is to allow interrupts within an TDMA schedule, in order to achieve better interrupt response times while maintaining a sufficient degree of temporal independence via monitoring. In this paper we propose a novel approach that optimizes the TDMA schedule based on the partitions internal timing behavior and tasks parameters. The developed optimization algorithm generates a maximum amount of slack within the TDMA cycle. This slack is later used to interpose interrupts, while maintaining the interference with a monitor. We show correctness of the approach and evaluate it in a hypervisor implementation.
Some experimental results on placement techniques (1976)

Seven placement algorithms - one constructive-initial placement algorithm and six iterative-improvement algorithms - were programmed and run on six problems ranging in size from 60 to 1300 modules. These problems included placing IC packs on a card, cards on a board and circuits on an LSI chip. It was found that the new force-directed pairwise relaxation algorithm was the best algorithm for the larger problems and was competitive with the other algorithms for the smaller problems. Other questions relating to placement strategies (such as, what are the advantages of constructive - initial start vs. random start and what is the best transformation of the placement problem to an associated quadratic assignment problem) are also answered.
Synthesis of 1-aryl(and 1-aralkyl)-β-carbolines (1967)

Some aromatic 1-aryl(and 1-aralkyl)-β-carbolines were prepared from their tetrahydro analogues by dehydrogenation over palladium on charcoal either in a Parr bomb or in refluxing toluene. The bomb reaction appears to be a better procedure for the preparations of aromatic β-carbolines with bulky C-1 substituents such as the naphthyl group. 1-(1-Naphthyl)- and 1-(2-naphthyl)-tetrahydro-β-carbolines were obtained by the cyclization of the Schiff bases N-(l-naphthylmethylene)- and N-(2-naphthylmethylene)-tryptamines, respectively. Other tetrahydro-β-carbolines with smaller C-1 substituents were prepared by the condensation of tryptamine with the appropriate aldehydes.