gebhart/patrepeval huggingface dataset:
Self-Propelled Carbohydrate-Sensitive Microtransporters with Built-In Boronic Acid Recognition for Isolating Sugars and Cells (2012)

A new nanomotor-based target isolation strategy, based on a "built-in" recognition capability, is presented. The concept relies on a poly(3-aminophenylboronic acid) (PAPBA)/Ni/Pt microtube engine coupling the selective monosaccharide recognition of the boronic acid-based outer polymeric layer with the catalytic function of the inner platinum layer. The PAPBA-based microrocket is prepared by membrane-templated electropolymerization of 3-aminophenylboronic acid monomer. The resulting boronic acid-based microengine itself provides the target recognition without the need for additional external functionalization. "On-the-fly" binding and transport of yeast cells (containing sugar residues on their wall) and glucose are illustrated. The use of the recognition polymeric layer does not hinder the efficient propulsion of the microengine in aqueous and physiological media. Release of the captured yeast cells is triggered via a competitive sugar binding involving addition of fructose. No such capture and transport are observed in control experiments involving other cells or microengines. Selective isolation of monosaccharides is illustrated using polystyrene particles loaded with different sugars. Such self-propelled nanomachines with a built-in recognition capability hold considerable promise for diverse applications.
Nitazoxanide Inhibits Biofilm Production and Hemagglutination by Enteroaggregative <i>Escherichia coli</i> Strains by Blocking Assembly of AafA Fimbriae (2010)

Enteroaggregative Escherichia coli (EAEC) strains have emerged as common causes of persistent diarrhea and malnutrition among children and HIV-infected persons. During infection, EAEC typically adheres to the intestinal mucosa via fimbrial adhesins, which results in a characteristic aggregative pattern. In the study described here we investigated whether the broad-spectrum antiparasitic and antidiarrheal drug nitazoxanide (NTZ) might be active against EAEC in vitro. While E. coli strains were resistant to NTZ in rich Luria-Bertani medium (MIC > 64 microg/ml), the drug was slightly inhibitory in a minimal medium supplemented with glucose (MinA-G medium; MIC, approximately 32 microg/ml). NTZ also inhibited biofilm production by strain EAEC 042 in both Dulbecco's modified Eagle's medium and MinA-G medium with a 50% inhibitory concentration of approximately 12 microg/ml. Immunofluorescence and immunoblot analyses with antibody against the major fimbrial subunit AafA of aggregative adherence fimbriae vaariant II (AAF/II) established that the numbers of AAF/II filaments on bacteria grown in the presence of NTZ were dramatically reduced. Comparative quantitative reverse transcription-PCR and reporter gene fusions (aafA::phoA) indicated that aafA expression was unaffected by NTZ, while aggR transcript levels and aggR::lacZ expression were increased approximately 10- and 2.5-fold, respectively, compared with that for the untreated controls. More generally, NTZ inhibited hemagglutination (HA) of red blood cells by the non-biofilm-producing strain JM221 expressing either AAF/I or type I fimbriae. Our findings suggest that the inhibitory action of NTZ on biofilm formation and HA is likely due to inhibition of fimbrial assembly. Antimicrobial agents that inhibit the assembly or function of fimbrial filaments should be good candidates for the prevention of infection.
Structural and biochemical basis for the UVB‐induced alterations in epidermal barrier function (1997)

Ultraviolet light (UVR) induces a myriad of cutaneous changes, including delayed disruption of the permeability barrier with higher doses. To investigate the basis for the UVB-induced barrier alteration, we assessed the epidermal lamellar body secretory system at various time points before and after barrier disruption with a single high dose of UVB (7.5 MED) to murine epidermis. Morphological data were correlated with changes in epidermal proliferation and lipid synthesis, indicative of lamellar body generation. Twenty-four hours following UVB, the stratum corneum (SC) is normal, but a layer of abnormal, vacuolated, and lamellar body (LB)-deficient cells is present, immediately beneath the stratum granulosum (SG)/SC interface. Immediately subjacent to this band of damaged cells, normal keratinocytes that contain intact LBs are present. By 72 h, concomitant with the appearance of a barrier abnormality, extensively damaged cells persist at the SC/SG interface, and abnormal lamellar membrane structures appear in the lower SC. Upper stratum spinosum (SS) and lower SG cells appear normal, with increased numbers of LBs. A barrier abnormality is still present at 96 h, in association with membrane abnormalities in the lower SC interstices, but up to four normal appearing, subjacent SG cell layers are present. By 120 h, accelerated LB formation and precocious LB extrusion occur throughout the thickened SG; normal lamellar membranes are present in the lower SC; and barrier recovery is almost complete. Whereas, epidermal synthesis of the major barrier lipid species (i.e., cholesterol, fatty acids, and ceramides, including acylceramides) is reduced or unchanged at 24 and 48 h, it increases significantly 72 h after exposure to UVB. Therefore, the delayed disruption of the permeability barrier following acute UVB exposure results from the arrival of a band of lamellar body-incompetent (i.e., damaged) cells at the SG/SC interface. The subsequent, rapid recovery of the barrier, in turn, results from compensatory hyperplasia of subjacent, undamaged SS/SG cells, generating increased numbers and contents of LB. These results underscore the critical role of the stratum compactum in mediating barrier function, and suggest that beneficial therapeutic effects of UV exposure may be due to enhanced lipid production and barrier regeneration.
Blind Channel Estimation for Orthogonal STBC in MISO Systems (2007)

This paper presents a closed-form blind channel estimation scheme for orthogonal space-time block codes in multiple-input single-output (MISO) systems, with specific focus on Alamouti's code for two transmit antennas. The channel matrix is estimated from the eigenvalue decomposition of the fourth-order cumulant matrix of the received signal. Unlike previous blind estimation schemes for MISO systems, the proposed algorithm is tested with block and slowly fading channels. The proposed scheme performs very well in both cases. A single pilot tuple is required to correctly assign the estimated to the actual channels and to resolve the sign ambiguity common to all blind estimators. It is shown that this scheme outperforms the only other available blind channel estimation scheme for this scenario. To achieve good performance in terms of bit error rate, 100-300 sample points are sufficient to provide accurate channel estimates. The main disadvantage of the proposed scheme is the complexity associated with the estimation of fourth-order cumulants. This complexity is reduced by exploiting the symmetry inherent in the cumulant matrix.
Optimising cryopreservation protocols for haematopoietic progenitor cells: a methodological approach for umbilical cord blood. (2006)

Current cryopreservation protocols for haematopoietic cells have developed largely empirically and there is no consensus on an optimal method of preservation. These protocols, though providing sufficient cells to permit engraftment, can lead to cell loss of the order of 50 percent. In the context of umbilical cord blood such losses are unacceptable. Whilst an empirical approach can provide an acceptable level of recovery, the cryopreservation process can only be optimised by adopting a methodological approach. This paper provides an overview of just such an approach as illustrated by a study on CD34 cells from umbilical cord blood. It involves firstly the determination of membrane permeability parameters that can then be used to model safe addition and elution protocols for the chosen cryoprotectant, in this case dimethyl sulphoxide. This in turn permits cryoprotectant toxicity to be evaluated free from the confounding effect of osmotic damage caused by inappropriate addition and elution protocols. Finally, non-toxic concentrations of cryoprotectant may be investigated in a cooling rate study to provide an optimal cryopreservation protocol. Using the model, the effect on CD34 cells of current addition and elution protocols was also examined.
Immobilization of transition metal (Fe2+, Co2+, VO2+ or Cu2+) Schiff base complexes onto graphene oxide as efficient and recyclable catalysts for epoxidation of styrene (2014)

Transition metal (Fe2+, Co2+, VO2+ or Cu2+) Schiff base complexes were immobilized onto graphene oxide previously functionalized with 3-aminopropyltriethoxysilane (3-APTES). X-ray diffraction (XRD), IR spectroscopy, thermal gravimetric analyses (TGA) and inductively coupled plasma atomic emission spectroscopy (ICP-AES) confirmed the successful incorporation of the metal Schiff base onto the graphene oxide. N2 adsorption–desorption, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed the intact structure of the graphene oxide. Catalytic results showed that the immobilized copper(II) Schiff base complex catalyst was more active than the immobilized iron(II), cobalt(II) and oxovanadium(IV) complexes in the epooxidation of styrene. Above 94% styrene conversion and excellent ∼99% selectivity to the epoxide could be achieved over the copper(II) Schiff base heterogeneous catalyst in the epoxidation of styrene using tert-butyl hydroperoxide (TBHP) as oxidant after 7 h reaction. The recycling experiment results indicated that the catalyst could maintain very high styrene conversion (>93%) and epoxide selectivity (>99%) even after being used for four cycles.
Ultrafast Multidimensional NMR: Principles and Practice of Single-Scan Methods (2008)

We have recently proposed a scheme enabling the collection of arbitrary 2-D NMR data sets within a single scan. This so-called ultrafast methodology operates by replacing the traditional t 1 temporal encoding with a spatial encoding of the indirect-domain spin interactions. Following a mixing period, the spatially encoded I(ω1) spectrum can be repeatedly read out using a train of oscillatory gradients, leading to a single, continuous FID containing an interferogram with the mixed-domain S(ω1, t 2) data. Rearrangement of the resulting data points followed by Fourier processing along the direct-domain axis can thereby lead to arbitrary 2-D I(ω1, ω2) spectra–within a single transient. This article describes the physical principles of this single-scan 2-D NMR procedure, and gives practical guidelines for its implementation in commercial instruments. Attention is paid on how to translate the spectral variables defining traditional 2-D NMR experiments into the timing and gradient parameters involved in single-scan 2-D acquisitions; on how to program the spatial encoding RF manipulations needed for the experiment's execution; and on the special processing aspects involved in this kind of acquisitions. The optimization of the various data collection and processing stages is discussed, and their implementations are illustrated with simple guiding examples and with state-of-the-art experimental results on proteins and nucleic acids.
Down‐regulation of collagen and connective tissue growth factor expression with hepatocyte growth factor in lung fibroblasts from white scleroderma patients via two signaling pathways (2007)

In lung fibroblasts from white SSc patients, HGF down-regulates the accumulation of CTGF via MAPK/MMP-1 and NF-kappaB signaling pathways, whereas collagen down-regulation is mediated mainly by a MAPK/MMP-1-dependent pathway.
X-ray gratings: the GMS mount (1979)

Using rigorous electromagnetic theory, the interest of the specific off-plane mounting for x-ray gratings is discussed in detail. The optimization of the blaze angle for ruled gratings is considered, as well as the extension of the mounting to sinusoidal and lamellar profiles.
Preparation and Properties of a Series of Halocarbonylrhodates (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation and Properties of a Series of HalocarbonylrhodatesL. M. VallarinoCite this: Inorg. Chem. 1965, 4, 2, 161–165Publication Date (Print):February 1, 1965Publication History Published online1 May 2002Published inissue 1 February 1965https://pubs.acs.org/doi/10.1021/ic50024a007https://doi.org/10.1021/ic50024a007research-articleACS PublicationsRequest reuse permissionsArticle Views205Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Search of new Antimitotics compounds from the Cuban flora (2011)

Detener la proliferacion celular es el principal proposito de la quimioterapia anticarcinogenica. Para ello se ha realizado una busqueda a partir de fuentes naturales de nuevos inhibidores efectivos que reaccionen con los componentes del citoesqueleto y puedan detener la division celular. En poblaciones que tradicionalmente utilizan plantas medicinales se estimula el uso de aquellas especies con accion quimiopreventivas como parte de una estrategia que contribuya a la calidad de vida. Los productos herbarios tienen costos significativamente mas bajos, exhiben poca o ninguna toxicidad durante la administracion oral a largo plazo y estan al alcance de todos. Nuestro trabajo consistio en realizar un tamizaje de 85 extractos de plantas medicinales cubanas seleccionadas en base al uso tradicional, en las encuestas etnobotanicas e informacion farmacologica (actividad antiparasitaria, antitumoral, abortiva, etc). La actividad antitubulinica fue evaluada mediante una version modificada del ensayo turbimetrico del ensamblaje/desensamblaje de la tubulina. Se determino la actividad limite de los nuevos agentes antitubulinicos siendo los extractos de Tamarindus indica L., Lawsonia inermes L and Xanthium strumarium L. los de mejor actividad antitubulinica segun las condiciones ensayadas
Antibody Conjugates with Unnatural Amino Acids (2015)

Antibody conjugates are important in many areas of medicine and biological research, and antibody-drug conjugates (ADCs) are becoming an important next generation class of therapeutics for cancer treatment. Early conjugation technologies relied upon random conjugation to multiple amino acid side chains, resulting in heterogeneous mixtures of labeled antibody. Recent studies, however, strongly support the notion that site-specific conjugation produces a homogeneous population of antibody conjugates with improved pharmacologic properties over randomly coupled molecules. Genetically incorporated unnatural amino acids (uAAs) allow unique orthogonal coupling strategies compared to those used for the 20 naturally occurring amino acids. Thus, uAAs provide a novel paradigm for creation of next generation ADCs. Additionally, uAA-based site-specific conjugation could also empower creation of additional multifunctional conjugates important as biopharmaceuticals, diagnostics, or reagents.
The influence of twelve weeks of aerobic training on the blood lipids of middle-aged men. (1991)

Lipoprotein profiles revealed a significant increase in total cholesterol between trained and control groups and an increase of HDL-C in both groups after exercise. There were no significant differences in resting cholesterol levels, nor in LDL-C after exercise in both groups. The results of the study suggest that improvement in work capacity as a result of training is not necessarily accompanied by changes in the lipid profile.
Howard Schwartz: an introduction (2002)

<p>Keine Beschreibung.</p>
Fluorescently labelled bovine acyl-CoA-binding protein acting as an acyl-CoA sensor: interaction with CoA and acyl-CoA esters and its use in measuring free acyl-CoA esters and non-esterified fatty acids (2002)

Long-chain acyl-CoA esters are key metabolites in lipid synthesis and beta-oxidation but, at the same time, are important regulators of intermediate metabolism, insulin secretion, vesicular trafficking and gene expression. Key tools in studying the regulatory functions of acyl-CoA esters are reliable methods for the determination of free acyl-CoA concentrations. No such method is presently available. In the present study, we describe the synthesis of two acyl-CoA sensors for measuring free acyl-CoA concentrations using acyl-CoA-binding protein as a scaffold. Met24 and Ala53 of bovine acyl-CoA-binding protein were replaced by cysteine residues, which were covalently modified with 6-bromoacetyl-2-dimethylaminonaphthalene to make the two fluorescent acyl-CoA indicators (FACIs) FACI-24 and FACI-53. FACI-24 and FACI-53 showed fluorescence emission maximum at 510 and 525 nm respectively, in the absence of ligand (excitation 387 nm). Titration of FACI-24 and FACI-53 with hexadecanoyl-CoA and dodecanoyl-CoA increased the fluorescence yield 5.5-and 4.7-fold at 460 and 495 nm respectively. FACI-24 exhibited a high, and similar increase in, fluorescence yield at 460 nm upon binding of C14-C20 saturated and unsaturated acyl-CoA esters. Both indicators bind long-chain (>C14) acyl-CoA esters with high specificity and affinity (K(d)=0.6-1.7 nM). FACI-53 showed a high fluorescence yield for C8-C12 acyl chains. It is shown that FACI-24 acts as a sensitive acyl-CoA sensor for measuring the concentration of free acyl-CoA, acyl-CoA synthetase activity and the concentrations of free fatty acids after conversion of the fatty acid into their respective acyl-CoA esters.
Cellular and genetic characterization of human adult bone marrow-derived neural stem-like cells: a potential antiglioma cellular vector. (2003)

We describe the in vitro isolation and expansion of cells capable of forming neurosphere-like aggregates from human adult bone marrow. Cells within these passaged spheroids can differentiate into astrocytes, specific neuronal subtypes, and oligodendrocytes and have gene expression profiles similar to human fetal brain-derived neural stem cells. Genetically modified neural-competent bone marrow-derived cells efficiently migrate toward distant sites of brain injury and tumor in vivo, where they differentiate and express therapeutic transgenes when transplanted into the brains of mice. These studies suggest that adult bone marrow may serve as a large reservoir for autologous neural stem-like cells for future therapeutic strategies.
Intelligent user interfaces in the living room (2003)

The purpose of this paper is to present our experience from the design of a personalized television application, and the implications for the design of interactive television applications in general. Personalized advertising is a gentle introduction to interactive television applications through a push paradigm that is closer to the established patterns of television use. While personalization is a practice widely used on the Internet, applying personalization techniques over digital television infrastructures presents significant obstacles, which we address with explicit design moves
RDS-ALERT : A DRIVE PROJECT TO DEVELOP A PROPOSED STANDARD FOR THE TRAFFIC MESSAGE CHANNEL FEATURE OF THE RADIO DATA SYSTEM RDS (1990)

RDS-ALERT (Radio Data System-Advice and Problem Location for European Road Traffic) is a project within the European Community's DRIVE research programme. The main goal of this project is to develop, and build European consensus upon, a draft standard for broadcasting traffic messages in densely coded digital form via the proposed Traffic Message Channel (TMC) feature of the radio data system RDS. The author outlines the background to this project, including the existing and proposed capabilities of RDS to assist traffic information broadcasting and gives a brief report on the progress to date. >
Infectious Diseases: Considerations for the 21st Century (2001)

The discipline of infectious diseases will assume added prominence in the 21st century in both developed and developing nations. To an unprecedented extent, issues related to infectious diseases in the context of global health are on the agendas of world leaders, health policymakers, and philanthropies. This attention has focused both on scientific challenges such as vaccine development and on the deleterious effects of infectious diseases on economic development and political stability. Interest in global health has led to increasing levels of financial support, which, combined with recent technological advances, provide extraordinary opportunities for infectious disease research in the 21st century. The sequencing of human and microbial genomes and advances in functional genomics will underpin significant progress in many areas, including understanding human predisposition and susceptibility to disease, microbial pathogenesis, and the development new diagnostics, vaccines, and therapies. Increasingly, infectious disease research will be linked to the development of the medical infrastructure and training needed in developing countries to translate scientific advances into operational reality.
Skunk Works type approach for F-SAT (1992)

The topics are presented in viewgraph form and include the following: the F-SAT Program, the classic program organization, design/configuration management, procurement/material, manufacturing, quality assurance, the facility, and personnel management.