gebhart/patrepeval huggingface dataset:
Characterization of cat dander-specific T lymphocytes from atopic patients. (1994)

Fel d I, the major cat dander allergen, is recognized by serum IgE of more than 80% of all cat-allergic patients. Because IgE synthesis by B lymphocytes is under the control of T lymphocytes, we studied the specificity and lymphokine production profiles of cat dander-specific T lymphocytes. Polyclonal cat dander-specific T cell lines were found to react with purified Fel d I, but not with cat albumin, the only other characterized cat allergen. Similarly, within a panel of CD4+ T lymphocyte clones (TLC) that was generated from these cat dander-specific T cell lines, 5 of 16 TLC were found to react with Fel d I, and 0 of 16 with cat albumin. The remaining 11 TLC were shown to recognize at least two different proteins. In general, the TLC had a high IL-4/IFN-gamma production ratio, and could recognize the cat dander extract in an HLA-DR, HLA-DQ, or HLA-DP restricted manner. In addition, five distinct T cell epitopes of Fel d I were identified by using a panel of overlapping synthetic peptides of both chains of Fel d I. The data presented here indicate that, even though multiple proteins in cat dander extract are recognized by T lymphocytes of allergic patients, Fel d I, the major IgE binding allergen, is also important in T cell activation. The fact that the cat-specific TLC are Th2-like indicates that these cells may play an important role in the pathophysiology of allergic responses to cat allergens. However, the diversity of HLA-class II restriction of cat dander- and Fel d I-specific TLC and the presence of multiple T cell epitopes in the allergen may complicate future immunotherapies.
Technology review - Biometrics-Technology, Application, Challenge, and Computational Intelligence Solutions (2007)

Biometrics is the science of the measurement of unique human characteristics, both physical and behavioral. Various biometric technologies are available for identifying or verifying an individual by measuring fingerprint, hand, face, signature, voice, or a combination of these traits. This paper aims to assist readers as they consider biometric solutions by examining common biometric technologies, introducing different biometric applications, and reviewing recent CI solutions presented at the 2006 IEEE WCCI
Iron Carbides: Preparation from Carbon-Rich Matrix (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIron Carbides: Preparation from Carbon-Rich MatrixMorton H. Litt and Shaul M. AharoniCite this: Ind. Eng. Chem. Prod. Res. Dev. 1971, 10, 2, 176–178Publication Date (Print):June 1, 1971Publication History Published online1 May 2002Published inissue 1 June 1971https://pubs.acs.org/doi/10.1021/i360038a013https://doi.org/10.1021/i360038a013research-articleACS PublicationsRequest reuse permissionsArticle Views99Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Morphology of DNA/single walled nanotubes complexes (2007)

International audience
Integrated cellular and ad hoc relaying systems: iCAR (2001)

Integrated cellular and ad hoc relaying systems (iCAR) is a new wireless system architecture based on the integration of cellular and modern ad hoc relaying technologies. It addresses the congestion problem due to unbalanced traffic in a cellular system and provides interoperability for heterogeneous networks. The iCAR system can efficiently balance traffic loads between cells by using ad hoc relaying stations (ARS) to relay traffic from one cell to another dynamically. This not only increases the system's capacity cost effectively, but also reduces the transmission power for mobile hosts and extends system coverage. We compare the performance of the iCAR system with conventional cellular systems in terms of the call blocking/dropping probability, throughput, and signaling overhead via analysis and simulation. Our results show that with a limited number of ARSs and some increase in the signaling overhead (as well as hardware complexity), the call blocking/dropping probability in a congested cell and the overall system can be reduced.
Kinetic and Structural Analysis of Bisubstrate Inhibition of the <i>Salmonella enterica </i>Aminoglycoside 6‘-<i>N</i>-Acetyltransferase<sup>,</sup> (2007)

Aminoglycosides are antibacterial compounds that act by binding to the A site of the small 30S bacterial ribosomal subunit and inhibiting protein translation. Clinical resistance to aminoglycosides is generally the result of the expression of enzymes that covalently modify the antibiotic, including phosphorylation, adenylylation, and acetylation. Bisubstrate analogs for the aminoglycoside N-acetyltransferases are nanomolar inhibitors of Enterococcus faecium AAC(6')-Ii. However, in the case of the Salmonella enterica aac(6')-Iy-encoded aminoglycoside N-acetyltransferase, we demonstrate that a series of bisubstrate analogs are only micromolar inhibitors. In contrast to studies with AAC(6')-Ii, the inhibition constants toward AAC(6')-Iy are essentially independent of both the identity of the aminoglycoside component of the bisubstrate and the number of carbon atoms that are used to link the CoA and aminoglycoside components. The patterns of inhibition suggest that the CoA portion of the bisubstrate analog can bind to the enzyme-aminoglycoside substrate complex and that the aminoglycoside portion can bind to the enzyme-CoA product complex. However, at the high concentrations of bisubstrate analog used in crystallization experiments, we could crystallize and solve the three-dimensional structure of the enzyme-bisubstrate complex. The structure reveals that both the CoA and aminoglycoside portions bind in essentially the same positions as those previously observed for the enzyme-CoA-ribostamycin complex, with only a modest adjustment to accommodate the "linker". These results are compared to previous studies of the interaction of similar bisubstrate analogs with other aminoglycoside N-acetyltransferases.
Combined topical flucytosine and amphotericin B for refractory vaginal Candida glabrata infections (2001)

Patients with vaginitis due to highly azole resistant Candida glabrata can be particularly difficult to treat. We describe three cases of longstanding vaginal candidiasis due to C glabrata. These had failed to respond to local and systemic antifungals. Flucytosine (1 g) and amphotericin B (100 mg) formulated in lubricating jelly base in a total 8 g delivered dose, was used per vagina once daily for 14 days with significant improvement, both clinically and microbiologically.
Cathepsin-Dependent Apoptosis Triggered by Supraoptimal Activation of T Lymphocytes: A Possible Mechanism of High Dose Tolerance (2004)

High doses of Ag can paradoxically suppress immune responses in vivo. This Ag-specific unresponsiveness (termed high dose tolerance) involves extrathymic mechanisms in mature T lymphocytes. To investigate these mechanisms, we used the in vitro model of PBL activated with anti-CD3 or PHA. In these conditions, increasing mitogen concentrations resulted in a reduction of the proliferative response, associated with an increased percentage of apoptotic cells. Apoptosis did not require prior exposure to IL-2, it was not the consequence of CD178/CD95 or TNF/TNFR interactions, and was therefore clearly distinct from activation-induced cell death. Although the pan-caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (zVAD-fmk) decreased DNA fragmentation, cytochrome c release and caspase-9 and caspase-3 activation were not implicated, suggesting that this apoptosis did not primarily involve the intrinsic mitochondrial pathway. E64d, a cysteine protease inhibitor, as well as specific inhibitors of cathepsin B and cathepsin L conferred protection. We further demonstrated that cathepsin B and cathepsin L were released from the lysosomes and catalytically active in the cytosol. Release of cathepsin B and cathepsin L was the consequence of lysosomal membrane permeabilization without complete disruption of the cytosol-lysosome pH gradient. These results demonstrate a role for cathepsins in supraoptimal activation-induced apoptosis in vitro and suggest their possible participation in high dose tolerance in vivo.
Immunoregulatory T cell circuits in man. Identification of a distinct T cell subpopulation of the helper/inducer lineage that amplifies the development of alloantigen-specific suppressor T cells. (1985)

Regulation of the immune response in man is dependent on interactions between cells of helper/inducer (Leu-3+/T4+) lineage and cells of suppressor/cytotoxic (Leu-2+/T8+) lineage. By using the mixed leukocyte reaction (MLR) as a model system, we have shown previously that alloantigen-primed Leu-3+ cells induce autologous Leu-2+ cells to differentiate into suppressor T cells that specifically inhibit the response of fresh T cells to the original allogeneic stimulator cells. The current study was undertaken to analyze the roles in this suppressor circuit of subpopulations of Leu-3+ cells distinguished from one another on the basis of their binding or lack of binding to monoclonal anti-Leu-8 antibody. Although both Leu-3+,8- and Leu-3+,8+ T cells proliferated in allogeneic MLR, alloactivated Leu-3+,8+ cells alone induced proliferation and differentiation of Leu-2+ suppressor cells. Leu-3+,8+ cells also induced Leu-3+,8- cells to proliferate, and following their activation in this manner, such autoactivated Leu-3+,8- cells augmented the differentiation of Leu-2+ suppressor cells, but only in the presence of alloactivated Leu-3+,8+ cells. Furthermore, this effect, like the suppressor effect, was specific for the inducer cells, and thus indirectly for the HLA-DR antigens of the original allogeneic stimulator cells as well. These results indicate that alloantigen-primed Leu-3+,8+ cells not only activate specific Leu-2+ suppressor cells but also activate specific Leu-3+,8- suppressor-amplifier cells, and in combination, these cells exert potent feedback inhibition of MLR.
Unexpected salt effects on charge separation yields in phenothiazine derivatives-methylviologen systems (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnexpected salt effects on charge separation yields in phenothiazine derivatives-methylviologen systemsYuji Kawanishi, Noboru Kitamura, and Shigeo TazukeCite this: J. Phys. Chem. 1986, 90, 22, 6034–6037Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/j100280a115https://doi.org/10.1021/j100280a115research-articleACS PublicationsRequest reuse permissionsArticle Views140Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Building a service provisioning system using the Enterprise JavaBean framework (2002)

This paper provides an overview of the Sun Microsystems Enterprise JavaBeans/sup TM/ application framework in the context of a service provisioning system for GTE's IP Telecom offerings. The provisioning system takes advantage of the capabilities of EJB to provide a scalable, robust system that supports easy introduction of new services. The paper further describes the strengths and weaknesses of EJB as discovered while implementing this provisioning system and presents the authors' approaches to the EJB shortcomings. It concludes with a discussion of the future directions of EJB and how the identified limitations are being addressed in future revisions.
Catalytic mechanisms of acyl transfer reactions in dipolar aprotic media. 2. Electrophilic activation of the carbonyl group by quaternary alkylammonium and imidazolium functions (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic mechanisms of acyl transfer reactions in dipolar aprotic media. 2. Electrophilic activation of the carbonyl group by quaternary alkylammonium and imidazolium functionsJoseph Hajdu and Georgianna M. SmithCite this: J. Am. Chem. Soc. 1981, 103, 20, 6192–6197Publication Date (Print):October 1, 1981Publication History Published online1 May 2002Published inissue 1 October 1981https://pubs.acs.org/doi/10.1021/ja00410a035https://doi.org/10.1021/ja00410a035research-articleACS PublicationsRequest reuse permissionsArticle Views335Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Voxel-Based Extraction of Transmission Lines From Airborne LiDAR Point Cloud Data (2018)

The safety of the electricity infrastructure significantly affects both our daily life and industrial activities. Timely and accurate monitoring of the safety of electricity network can prevent dangerous situations effectively. Thus, we, in this paper, develop a voxel-based method for automatically extracting the transmission lines from airborne LiDAR point cloud data. The method proposed in this paper uses three-dimensional (3-D) voxels as primitives and consist of the following steps: First, skeleton structure extraction using Laplacian smoothing; second, feature construction of a 3-D voxel using Latent Dirichlet allocation topic model; and third Markov random field model-based extraction for generating locally continuous and globally optimal results. To evaluate the effectiveness and robustness of the proposed method, experiments were conducted on four different types of power line scenes with flat and complex terrains from helicopter-borne LiDAR point cloud data. Experimental results demonstrate that our proposed method is efficient and robust for automatically detecting both the single conductor and the bundled conductors, with precision, recall, and quality of over 96.78%, 98.67%, and 96.66%, respectively. Moreover, compared with other existing methods, our proposed method provides higher detection correctness rate.
Fractional finite Fourier transform (2004)

We show that a fractional version of the finite Fourier transform may be defined by using prolate spheroidal wave functions of order zero. The transform is linear and additive in its index and asymptotically goes over to Namias's definition of the fractional Fourier transform. As a special case of this definition, it is shown that the finite Fourier transform may be inverted by using information over a finite range of frequencies in Fourier space, the inversion being sensitive to noise. Numerical illustrations for both forward (fractional) and inverse finite transforms are provided.
Parotid secretory protein is expressed and inducible in human gingival keratinocytes (2005)

The present study has demonstrated for the first time that PSP is expressed in keratinocytes and that it can be up-regulated by bacteria and humoral factors. Thus PSP may have a role in the innate defense system at the gingival epithelial surface.
Genotyping and detection of common avian and human origin-influenza viruses using a portable chemiluminescence imaging microarray (2016)

A reliable CL detection oligonucleotide microarray had been developed to genotype and detected these influenza viruses.
Distributed Embedded Real-Time Systems and Beyond: A Vision of Future Road Vehicle Management (2008)

This paper presents a technical vision for future individual traffic. It deals with two different objectives: passenger cars or motorcycles as battery-driven electric vehicles (EVs) and traffic congestion avoidance. On the technical background of our own work we will explain how power supply for recharging the batteries will have to be organized in a distributed fashion, in particular under the assumption that the power is provided through renewable sources such as from wind turbines and solar panels (which are widely dispersed themselves). We will argue that while the unpredictability of local or regional customers in traditional power grid management creates already major problems for network stability (thus for providing the reserve energy needed) these will be greatly amplified by introducing EVs on a large scale, and by integrating renewable energy into the existing power management. In our DEZENT project we have defined and broadly pursued a distributed bottom-up approach for negotiating demand and supply under such circumstances, in an adequate architecture where demand and supply will be negotiated by software agents within 0.5 sec intervals while at the same time the grid stability is guaranteed. Since EVs themselves constitute relevant sources of reserve energy when coming up in large numbers they could be a core instrument for minimizing the stability problem.- Under this innovative perspective we will also discuss a novel distributed algorithm (BeeJamA) based on Swarm Intelligence where the EVs receive directions in due time, in a highly dynamic way before reaching each road intersection. In the absence of global information congestions are avoided and at the same time the travel times of all drivers are "homogenized". Combined with the transition into EV traffic we also could expect a very substantial reduction of pollution thus altogether an enormous ecological and economic progress.
Distributed Error Correction Coding Scheme for Low Storage Blockchain Systems (2020)

This article presents a novel way to reduce blockchain nodes’ memory requirements using error correcting codes. In particular, LDPC codes are taken as examples to explicitly demonstrate the scheme. The proposed coding scheme encodes data across multiple blocks, respectively, block headers, in the blockchain. This leads to a significant reduction in required memory at each node. We then apply the proposed coding technique to blockchains organized in two different ways. Our first scheme has the same protocol for mining, broadcasting, and verification of blocks, as Bitcoin-type blockchains. Our scheme is different in that <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">full nodes</i> do not have to store all blocks. Instead they will need to store only one block of a group of <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$t$ </tex-math></inline-formula> blocks. In the second scheme, we consider a new block verification protocol and an account-based model under the assumption that transmission between any two nodes can be established, as well as the broadcast transmission. Our block verification protocol uses the Byzantine fault tolerance algorithm and requires sending a newly mined block to only a small number of verification nodes, instead of broadcasting it to the entire network, which leads to a reduction of the network load.
Fingers-Closing and Other Rapid Conformational Changes in DNA Polymerase I (Klenow Fragment) and Their Role in Nucleotide Selectivity (2008)

We have developed a FRET-based assay for the fingers-closing conformational transition that occurs when a binary complex of DNA polymerase I (Klenow fragment) with a primer-template binds a complementary dNTP and have used this and other fluorescence assays to place the fingers-closing step within the reaction pathway. Because the rate of fingers-closing was substantially faster than the rate of nucleotide incorporation measured in chemical quench experiments, fingers-closing cannot be the rate-limiting prechemistry step defined by earlier kinetic studies. Experiments using Ca (2+) instead of Mg (2+) as the metal cofactor suggest instead that the prechemistry step may involve a change in metal ion occupancy at the polymerase active site. The use of ribonucleotide substrates shows there is a base discriminating step that precedes fingers-closing. This earlier step, detected by 2-AP fluorescence, is promoted by complementary nucleotides (ribo- as well as deoxyribo-) but is blocked by mismatches. The complementary rNTP blocks the subsequent fingers-closing step. Thus, discrimination against rNTPs occurs during the transition from open to closed conformations, whereas selection against mismatched bases is initiated earlier in the pathway, in the open complex. Mismatched dNTPs accelerate DNA release from the polymerase, suggesting the existence of an early intermediate in which DNA binding is destabilized relative to the binary complex; this could correspond to a conformation that allows an incoming dNTP to preview the template base. The early kinetic checkpoints identified by this study provide an efficient mechanism for the rejection of mismatched bases and ribose sugars and thus enhance polymerase throughput.
Substrate coupling: modeling, simulation and design perspectives (2004)

The finite impedance of silicon substrates has several consequences for the design and performance of ICs. In this paper, we discuss the state of the art in the areas of modeling and simulation of these effects. An overview of various modeling techniques is presented, with emphasis on integral-equation based boundary-element techniques. Numerical stability issues related to these techniques are discussed from a physical viewpoint. The impact on circuit design is considered by the means of specific examples.