gebhart/patrepeval huggingface dataset:
Crystallization of the N-terminal domain of human sex hormone-binding globulin, the major sex steroid carrier in blood (1999)

The amino-teminal laminin G-like domain of human sex hormone-binding globulin (SHBG), which contains the steroid-binding site and the dimerization domain, has been produced in Escherichia coli, purified to homogeneity and crystallized in complex with 5alpha--dihydrotestosterone (DHT) in two different crystal forms. Native data sets have been collected for tetragonal crystals (space group P4(1)22 or P4(3)22; unit-cell parameters a = 52.2, c = 148.4 A) diffracting to 3.3 A and trigonal crystals (R32; a = 104.0, c = 84.4 A) diffracting to better than 1.6 A. Since both crystal forms can only accommodate a single monomer in the asymmetric unit and share twofold rotational symmetry, it is proposed that the homodimer of this truncated form of SHBG, as observed in ultracentrifugation experiments, displays C(2) point-group symmetry.
Herpes Simplex Virus Type 1 Amplicon Vectors with Glucocorticoid-Inducible Gene Expression (1995)

A glucocorticoid-inducible transcription unit, composed of reiterated steroid-responsive cis elements, a synthetic promoter, and the human growth hormone gene (hGH), was inserted into herpes simplex virus type 1 (HSV-1) amplicon vectors to determine if regulated expression can be achieved in cell lines and primary hepatocytes. Initial constructs were prepared in a HSV amplicon vector that contained a 0.96-kb DNA fragment encompassing an origin of DNA replication of the short repeat (ORIs) of HSV-1, similar to ORIs-containing fragments used in many of the existing HSV amplicon vectors. In the absence of glucocorticoids, these constructs produced a high basal level of hGH expression in cells both transfected with naked amplicon DNA and infected with packaged amplicon virus. To lower basal expression, the larger ORIs was replaced with a 237-bp ORIs DNA fragment that was devoid of the many trans-activation elements flanking the larger ORIs, but that could still support DNA replication of amplicon vectors. HSV amplicon vectors with this smaller ORIs produced up to 30-fold hGH induction upon glucocorticoid treatment in virally transduced 293 cells, and up to 50-fold induction in transduced primary rat hepatocytes. The kinetics of hGH accumulation after induction and the dependence of hGH expression with respect to dexamethasone concentration were characterized in both cell types. These vectors have application for experiments in which highly efficient gene transfer and inducible gene expression are required.
Improvement of insulin sensitivity and β‐cell function by nateglinide and repaglinide in type 2 diabetic patients – a randomized controlled double‐blind and double‐dummy multicentre clinical trial (2006)

The efficacy of repaglinide and nateglinide in FBG, postprandial glucose excursion and early-phase insulin secretion is similar. But the effect of repaglinide 1.0 mg t.i.d. on HbA(1c) is stronger than that of nateglinide 90 mg t.i.d.. This trial had shown that nateglinide and repaglinide could comparably improve insulin sensitivity and beta-cell function.
Palladium-catalyzed N-(2-pyridyl)sulfonyl-directed C(sp<sup>3</sup>)–H γ-arylation of amino acid derivatives (2012)

The direct Pd-catalyzed γ-arylation of amino acid esters bearing a removable N-(2-pyridyl)sulfonyl directing group is described. A variety of N-(2-pyridyl)sulfonamide amino acid derivatives, including α-quaternary amino acid and β-amino acid substrates, react with iodoarenes in the presence of Pd(OAc)2 to provide γ-arylated products in synthetically useful yields. An unprecedented remote C(sp3)–H arylation of dipeptides is presented, illustrating the compatibility of the method with the presence of the peptidic bond. The process occurs without racemization at the Cα center and the auxiliary controlling group can be easily installed and removed in the amino acid backbone. A bimetallic PdII γ-metalated complex has been isolated and characterized showing the key role exerted by the (2-pyridyl)sulfonyl unit.
Determination of absolute configuration of chiral molecules using vibrational optical activity (2012)

Determination of absolute configuration (AC) of chiral molecules is an important step in any field related to chirality, especially in the pharmaceutical industry. Vibrational optical activity (VOA) has become a powerful tool for the determination of absolute configuration of chiral molecules in solution state after 30 years of evolution. VOA offers a novel alternative to X-ray crystallography, permitting AC determinations on neat liquid, oil, and solution samples. By comparing the sign and intensity of the experimental VOA spectrum with the ab initio DFT calculated VOA of a chosen configuration, one can unambiguously assign the absolute configuration of a chiral molecule. Comparing measured VOA spectra with calculated VOA spectra of all the conformers can also provide solution-state conformational populations. VOA consists of vibrational circular dichroism (VCD) and Raman optical activity (ROA). VCD is used routinely by researchers of various backgrounds including basic study of chirality, asymmetric synthesis or catalysis, drug screening, pharmacology and natural products. Although the application of ROA in AC determination lags behind that of the VCD, with the recent implementation of ROA calculation method into the commercial calculation software, ROA will in the future complement VCD for AC determination. In this presentation basic principles of the application of VCD to the determination of absolute configuration in chiral molecules are described. The steps required for VCD spectral measurement and calculation are outlined followed by recent applications of AC in small organic, pharmaceutical and natural product molecules.
Immunochemical Study of Epidermal Growth Factor in Rats with Mercuric Chloride-Induced Acute Renal Failure (1994)

Urine contains high concentrations of epidermal growth factor (EGF), and EGF is a potent growth promoter for proximal tubular cells. In the present study, urinary and whole-kidney EGF levels were investigated in rats with mercuric chloride (HgCl2)-induced acute renal failure (ARF) using a specific radioimmunoassay for rat EGF to clarify changes in EGF after toxic injury. Male Wistar rats were given HgCl2 (2 mg/kg, subcutaneously) or saline. Serum urea nitrogen and creatinine levels were measured for 4 days after toxin administration. Forty-eight hours after toxic injury, urinary immunoreactive EGF levels in HgCl2-treated rats decreased significantly compared with control rats (1.62 +/- 0.15 versus 3.78 +/- 0.21 ng/mg creatinine; p < 0.01). Urinary immunoreactive EGF was at its lowest level 96 h after toxic injury (0.64 +/- 0.06 ng/mg creatinine; p < 0.001). Twenty-four hours after toxic injury, renal immunoreactive EGF levels increased significantly compared with control rats (22.04 +/- 2.12 versus 4.84 +/- 0.70 ng/g wet weight tissue; p < 0.001), and the increase persisted for as long as 48 h (13.36 +/- 1.61 ng/g wet weight tissue; p < 0.05). In summary, urinary immunoreactive EGF levels decreased and renal EGF levels increased in rats with HgCl2-induced ARF. These findings suggest that there is an impairment in the excretion of EGF in rats with HgCl2-induced ARF and that local paracrine production of EGF continues in ARF.
Robotic Automated External Defibrillator Ambulance for Emergency Medical Service in Smart Cities (2016)

Smart cities essentially require the state-of-the-art technologies that can provide smart service in various aspects, and robotic systems are one of the key solutions for such requirements. Time is a critical issue when dealing with people who experience a sudden cardiac arrest that unfortunately could die due to inaccessibility of the emergency treatment. Therefore, an immediate treatment using automated external defibrillator (AED) must be administered to the victim within a few minutes after collapsing. Hence, we have designed and developed the ambulance robot, which brings along an AED in a sudden event of cardiac arrest and facilitates various modes of operation from manual to autonomous functioning to save someones lives in smart cities. The details of the design and development of such robot are presented in this paper.
Nematic tribenzosilatranes (2005)

Abstract The linkage of benzylsilatrane groups to laterally functionalized mesogens allows the investigation of molecular systems which contain propeller shaped mesogens and exhibit nematic phase behaviour.
Simultaneous multi‐site EPR spectroscopy <i>in vivo</i> (1993)

A gradient technique to measure electron paramagnetic resonance spectra simultaneously at several different locations is described. The technique is based on the use of point probes containing paramagnetic centers. The value of the magnetic field gradient is chosen to be sufficient to separate the EPR signals from the different paramagnetic probes yet at the same time small enough to change only minimally the shape of individual signals. The conditions to apply this technique are considered in detail. When experimental data have a high signal-to-noise ratio, the lineshape distortion induced by the gradient can be corrected with the aid of a known distribution function of paramagnetic centers within the probe. The maximum entropy deconvolution algorithm is successfully applied for the correction of significantly distorted lines. The technique is experimentally tested and applied to measure the concentration of oxygen in hypertrophied rat myocardium and normal rat kidney in vivo by low frequency EPR (L-band, 1.2 GHz). Two types of EPR oxygen-sensitive probes--lithium phthalocyanine crystals and synthetic carbohydrate chars--were used.
Grwoth Inhibition of Mouse MXT Mammary Tumor by the Luteinizing Hormone-Releasing Hormone Antagonist SB-75 (1990)

Female BDF1 mice bearing MXT mammary adenocarcinomas were treated for 3 weeks with the luteinizing hormone-releasing hormone (LH-RH) antagonist [Ac-D-Nal(2)1, D-Phe(4Cl)2, D-Pal(3)3, D-Cit6, D-Ala10]-LH-RH (SB-75), with the agonist D-Trp6-LH-RH, with tamoxifen (5 micrograms per animal per day subcutaneously), with the combination of D-Trp6-LH-RH and tamoxifen, or by surgical ovariectomy. SB-75 and D-Trp6-LH-RH were administered in the form of microcapsules releasing 25 micrograms/day. The reduction in tumor weights after treatment with SB-75, D-Trp6-LH-RH, D-Trp6-LH-RH plus tamoxifen, or ovariectomy was 84%, 64%, 33%, and 67%, respectively. Tamoxifen alone was ineffective. Histologically, the regressive changes in the treated tumors were characteristic of apoptosis (programmed cell death). In view of its potency and its immediate inhibitory effect, the LH-RH antagonist SB-75 should be considered as a possible new hormonal agent for the treatment of breast cancer.
The Critical Role of the Stem Region as a Functional Domain Responsible for the Oligomerization and Golgi Localization of N-Acetylglucosaminyltransferase V (2001)

We demonstrated that a region in the stem of N-acetylglucosaminyltransferase V (GnT-V), a Golgi resident protein, is not required for enzyme activity but serves as functional domain, responsible for intracellular localization. Deletion of the domain led to complete retention of the kinetic properties but resulted in the cell surface localization of the enzyme as well as its efficient secretion into the medium. The lack of this domain concomitantly abolished the disulfide-mediated oligomerization of GnT-V, which appears to confer the Golgi retention. When the domain was inserted into the stem region of a cell surface-localized type II membrane protein, the resulting chimeric protein was substantially oligomerized and predominantly localized in the intracellular organelle. Furthermore, it was found that the presence of this domain is exclusively responsible for homo-oligomer formation. This homophilic interaction appears to involve a hydrophobic cluster of residues in the alpha-helix of the domain, as indicated by secondary structure predictions. These findings suggest that the domain specifically participates in the Golgi retention of GnT-V, probably via inducing homo-oligomer formation, and would also provide a possible mechanism for the oligomerization, which is critical for localization in the Golgi.
Continuous <scp>D</scp>‐Fructose Dehydration to 5‐ Hydroxymethylfurfural Under Mild Conditions (2012)

The dehydration of D-fructose to 5-hydroxymethylfurfural was studied under single-phase conditions in the low boiling solvent 1,4-dioxane at moderate temperatures in the presence of the solid acid-catalyst Amberlyst-15. The reaction was first examined and optimized under batch conditions, where it was found that the yield could be increased up to 75 % by adding small amounts of DMSO. Subsequently, the reaction was performed under continuous flow in a fixed bed reactor. Internal and external mass transfer limitations could be eliminated by changing the particle size and by adjusting the flow rate. Under continuous conditions, the HMF yield could be further increased to 92 %; the space-time yield was found to be 75 times higher compared to the batch case. A long-term stability test (96 h), including solvent regeneration, demonstrated that the catalyst is stable over time. Additionally, it was shown that even small amounts of water have a negative effect on the HMF yield. Overall, the present system shows a good alternative to other systems presented in literature because high space-time yields and selectivities were obtained under relatively mild and continuous conditions.
Cloning, Chromosomal Localization and Functional Expression of the Gene Encoding the α‐Subunit of the cG‐MP‐Gated Channel in Human Cone Photoreceptors (1997)

Cyclic nucleotide-gated (CNG) ion channels serve as final targets of signal transduction in vertebrate photoreceptors. While the basic mechanisms of phototransduction are similar in rod and cone photoreceptors, both cell types express distinct sets of components of the transduction pathway. We report here the cloning of the cDNA encoding the alpha-subunit of the cGMP-gated channel of human cone photoreceptors. The open reading frame predicts a polypeptide of 694 amino acid residues with conserved functional parts and amino acid positions typical for the alpha-subunit of CNG-channels. Heterologous expression of the cDNA in Xenopus oocytes gave rise to cGMP-gated channel activity. Antiserum directed against the C-terminus of the bovine cone CNG channel alpha-subunit crossreacted specifically with the heterologously expressed polypeptide and stained cone photoreceptors and weakly also the outer plexiform layer in human retinal sections. Northern blot analysis detected a prominent mRNA species of approximately 3.8 kb in human retina. The entire gene spans approximately 30 kb of genomic sequence and is located on the pericentric band q11.2 of human chromosome 2. The gene is composed of seven exons, with introns located at positions which are preserved with respect to the human rod gene, indicating a common ancestral gene structure. RT-PCR analysis gave no evidence for alternatively spliced transcripts.
Eradication of Low-Level Methicillin-Resistant <i>Staphylococcus aureus</i> Skin Colonization with Topical Mupirocin (1988)

Methicillin-resistant Staphylococcus aureus (MKSA) infections have become a major problem in many US hospitals in the last ten years.‘,’ Attempts to solve the problem have been hampered by many factors. One of these factors is the difficulty in eradicating the organism from colonized sites and thereby removing one of the reservoirs of endemicity. Another complicating factor is the lack of consistency in the published literature regarding what constitutes eradication and, therefore, when it is appropriate to discontinue isolation of patients or work restrictions of personnel. Even the decisions regarding when and how often to culture personnel remain difficult to define.” Finally, although the Australian experience illustrated an environmental component to an MRSA outbreak, the role of the environment in MRSA infections remains unclear. The following report describes the measures utilized to eradicate low-level MRSA skin colonization in a long-term psychiatric patient and illustrates several problems of-long-term MRSA carriage in institutionalized patients.
Protease-catalyzed synthesis of Leu-enkephalin in a solvent-free system. (1996)

The total enzymatic synthesis of a model peptide Leu-enkephalin on a preparative scale was accomplished in the so-called solvent-free system. The syntheses were carried out in a rotary glass homogenizer by admixing solid reactants with native proteases and Na2CO3.10H2O. The most feasible way leading to biologically active Leu-enkephalin, was based on the strategy of 2 + (1 + 2) condensation catalyzed by alpha-chymotrypsin, thermolysin and papain for the final segment coupling. Subtilisin was used for the ester hydrolysis of peptide intermediates. Alternative strategies as well as the influence of several reaction conditions on the yield of the protease-catalyzed synthesis of Leu-enkephalin or Leu-enkephalin amide were also investigated.
Measurement of substrate current in SOI MOSFETs (1994)

A new "Quasi-SOI" MOSFET structure is shown to allow direct measurement of substrate current in a fully-depleted SOI device. The holes generated by impact ionization near the drain are collected at the substrate terminal after they have traversed the source-body barrier and caused bipolar multiplication. By monitoring this hole current, direct characterization of the impact-ionization multiplication factor, M, and the parasitic bipolar gain, /spl beta/, was performed. It was found that M/spl minus/1 increases exponentially with V/sub DS/ and decreases with V/sub GS/, exhibiting a drain field dependence. The bipolar gain /spl beta/ was found to be as high as 1000 for V/sub GS//spl minus/V/sub T/=0 V and V/sub DS/=/spl minus/2.5 V, but decreases exponentially as V/sub DS/ increases. Finally, it was found that /spl beta/ also decreases as V/sub GS/ increases.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Design and Operation of Aircraft to Minimize Their Sonic Boom (1974)

Means of reducing or eliminating the sonic boom through aerodynamic design or aircraft operation are discussed. These include designing aircraft to minimize or eliminate certain features of the overpressure signature, operating aircraft at slightly supersonic speeds so that the sonic boom does not reach the ground, and seeking reductions through the high-altitude, high-speed flight conditions of hypersonic transports. A simple computer program has been developed that determines the area development of the equivalent body of revolution required to minimize various sonic boom signature parameters.
AlGaAs/GaAs HBT IC's for high-speed lightwave transmission systems (1992)

The implementation of multigigabit-per-second optical communication systems requires many high-speed electronic circuit components that meet stringent performance requirements. Several important research prototype circuits for fiber-optic transmission, implemented in a baseline AlGaAs/GaAs HBT process, are discussed. These include a 20 Gb/s decision circuit, a 27 Gb/s 1:2 demultiplexer, a 30 GB/s 2:1 multiplexer, a 27 Gb/s 4:1 multiplexer, and a 11 Gb/s laser driver IC.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Duality in the chemical character of secondary phosphine oxides (1966)

I. G. M. Campbell and I. D. R. Stevens, Chem. Commun. (London), 1966, 505 DOI: 10.1039/C19660000505
Profile extrusion and mechanical properties of crosslinked wood–thermoplastic composites (2006)

Abstract Challenges for wood‐thermoplastic composites to be utilized in structural applications are to lower product weight and to improve the long‐term load performance. Silane crosslinking of the composites is one way to reduce the creep during long‐term loading and to improve the mechanical properties. In this study, silane crosslinked wood‐polyethylene composites were produced by reactive extrusion and subsequently manufactured into rectangular profiles. The silane crosslinked composites were stored in a sauna at 90 °C to increase the degree of crosslinking. The toughness of the silane crosslinked composites was significantly higher than for the non‐crosslinked composites. Improved adhesion between the wood and polyethylene phases is most likely the reason for the improved toughness of the crosslinked composites. There was no significant difference in flexural modulus between the crosslinked and non‐crosslinked composites. In addition, impact testing showed that the impact strength of the crosslinked composites was considerable higher (at least double) than the non‐crosslinked. The effect of temperature on the impact strength of the composites indicated slightly higher impact strength at −30 °C than at 0° and at 25 °C, and then an incrase in impact strength at 60 °C. Crosslinking also reduced the creep response during short‐term loading. Moreover, scanning electron microscopy on the fracture surface of the crosslinked composites revealed good adhesion between the polyethylene and wood phases. POLYM. COMPOS. 27:184–194, 2006. © 2006 Society of Plastics Engineers
Chippe: a system for constraint driven behavioral synthesis (1990)

The Chippe system for constrained behavioural architecture synthesis uses a novel closed-loop design iteration technique in which the present state of the design is analyzed with respect to the goals and then modified for the next iteration. In this way the design state is iteratively driven towards meeting the global constraints imposed by the designer. The design synthesis is performed by a set of algorithmic tools specially constructed to permit the imposition of a wide variety of local constraints, and by a rule-based system which makes design analysis and modification decisions to set these local constraints. Key to these decisions is a design evaluator which examines the present state of the design and interactively reports its findings to the rule-based system. The closed-loop iteration strategy, the interaction between the rule base and the tools, and the evaluation performed to support the design decisions are detailed. Also presented are results from sample designs, including designs for the TMS320 digital signal processor chip.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
On comparing the symmetrical and non-symmetrical selective harmonic elimination pulse-width modulation technique for two-level three-phase voltage source converters (2010)

Selective harmonic elimination pulse-width modulation (SHE-PWM) techniques offer a tight control of the harmonic spectrum of a given voltage waveform generated by a power electronic converter along with a low number of switching transitions. These optimal switching transitions can be calculated through Fourier theory, and for a number of years quarter-wave and half-wave symmetries have been assumed when formulating the problem. It is shown recently that symmetry requirements can be relaxed as a constraint. This changes the way the problem is formulated and different solutions can be found. This study presents different formulations of the problem, namely quarter-, half-wave symmetry and non-symmetrical waveform. A critical evaluation and comparison between the three schemes are reported here based on various aspects, such as number of eliminated harmonics, harmonic spectrum profile, harmonic phasing, converter performance, computational time and solution space limitations. Selected simulation and experimentally validated results are presented to confirm the theoretical work of this study. This manuscript is a revised and extended version of earlier paper that was presented at the IEEE PESC 2008, Rhodes, Greece, 15–19 June 2008, and has not been submitted to any other journal for consideration and possible publication.