gebhart/patrepeval huggingface dataset:
Purification of IgG and albumin from human plasma by aqueous two phase system fractionation (2012)

The current shortages in human plasma products at global levels justify the development of new, cost effective plasma fractionation methods. We have developed a fractionation process to obtain immunoglobulin G (IgG) and albumin-enriched fractions based on polymer-salt aqueous two phase system (ATPS). A small-scale (0.02 L) ATPS composed of polyethyleneglycol 3350 (PEG), potassium phosphate and sodium chloride, at pH 6.1, was evaluated and subjected to 50-fold scale-up (1 L). Further purification of the fractions was performed using caprylic acid precipitation and ion exchange chromatography. Similar yield and purity were obtained at both small and large scales. IgG precipitated in the PEG rich upper phase at 83% recovery and 2.75-fold purification factor. An 81% pure albumin fraction was obtained in the salt rich bottom phase with a 91% yield. After polishing, IgG was obtained at a recovery of 70% and a purity of 92%. Corresponding values for albumin were 91% and 90%. This IgG and albumin fractionation technology deserves further evaluation as it may represent a potential alternative to conventional plasma fractionation methods.
The space groups of point group <i>C</i> <sub>3</sub>: some corrections, some comments (2002)

A survey of the October 2001 release of the Cambridge Structural Database [Cambridge Structural Database (1992). Cambridge Crystallographic Data Centre, 12 Union Road, Cambridge, England] has uncovered approximately 675 separate apparently reliable entries under space groups P3, P3(1), P3(2) and R3; in approximately 100 of these entries, the space-group assignment appears to be incorrect. Other features of these space groups are also discussed.
High-frequency operation of a planar-type microtransformer and its application to multilayered switching regulators (1990)

The operation of a multilayered microtransformer composed of planar zig-zag coils and amorphous magnetic film is described. The transformer has a maximum efficiency of 77.5%. Its equivalent circuit is approximated by the parallel connection of the winding inductance and of the stray capacitance. Variable magnetic coupling is obtained between the primary and secondary windings by shifting the relative position of the two coils. The microtransformer is used in a magnetically controlled multilayered switching regulator. The regulator has an output of 1.4 W and an efficiency of 24%. The magnetization loss in the circuit is the same as that of the semiconductors. A two-output-type multilayered switching regulator is also proposed that has an acceptably good output characteristic at each port even though a common magnetic film is used.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Osmotic stress induces variation in cellular levels of ornithine decarboxylase-antizyme (1998)

The polyamines, and especially putrescine, play an integral role in the physiological response of cells to varying extracellular osmotic conditions. Ornithine decarboxylase (ODC) synthesis and stability, as well as the activity of the polyamine transporter, had all been reported to be very sensitive to media osmolarity in different cells and tissues, yet the mechanism of this complex, co-ordinated response was not known. In this study we have determined that all these aspects of osmotic-shock response may be mediated by the common regulatory protein, ODC-antizyme. HTC cells were induced for antizyme and then exposed to media of reduced osmotic strength. Both antizyme activity and protein decreased rapidly, under these conditions, to new steady-state levels that depended upon the degree of reduction in media tonicity. This antizyme reduction was found to be due to a rapid increase in antizyme degradation, with a half-life decrease from 75 min down to 45 min occurring immediately upon exchanging media. In complementary experiments, increased media tonicity induced elevated antizyme levels and stability. The sensitivity of antizyme turnover to osmotic conditions was also observed in DH23b cells, which contain elevated levels of more stable antizyme. Interestingly, the two main antizyme proteins, AZ-1 and AZ-2 (presumably products from the first and second translational start sites), differed in their responses to these changing osmotic conditions. Just as feedback regulation of antizyme synthesis provides an effective mechanism for maintaining stable polyamine levels, these studies suggest that alteration in the rate of antizyme degradation may be the mechanism whereby cells adjust steady-state polyamine levels in response to stimulation or stress.
Proteinase-Activated Receptor-2: Physiological and Pathophysiological Roles (2003)

Protease-activated receptor 2 (PAR2) is the second member of a new subfamily of G-protein coupled receptors: the protease-activated receptors (PARs). At present, four different PARs have been cloned and all of them share the same basic mechanism of activation. A serine protease cleaves the extended, extracellular N-terminus of the receptor at a specific site within the protein chain to expose an N-terminal tethered ligand domain, which binds to and activates the cleaved receptor. In this manner, trypsin and mast cell β-tryptase activate PAR2. PARs are single use receptors because proteolytic activation is irreversible and the cleaved receptors are degraded in lysosomes. Thus, PARs play important roles in emergency situations, such as trauma and inflammation. Emerging evidence indicates that PAR2 is involved in the cardiovascular, pulmonary and gastrointestinal systems, where it controls inflammation and nociception. Work with selective agonists and knockout animals suggests a contribution of PAR2 to certain inflammatory diseases. Therefore, selective antagonists or agonists of these receptors may be useful therapeutic agents for the treatment of human diseases.
Axial Image Superposing (Super-FLEX) Effect Using the Mask Modulation Method for Optical Lithography (1991)

Phase and amplitude modulation in a mask can achieve amplitude superposition for multiple images along the light axis and phase control between them. This improves the depth of focus while keeping high resolution under high coherence illumination in optical lithography. This method explains the mechanism of the depth-of-focus enhancement effect in “peripherally added” or “edge-enhancing” phase-shifting masks and provides the most effective phase-shifter arrangement. Because a high coherency is required in this method, however, the proximity effect becomes a serious problem in practical LSI patterns.
Adjoint LMS: an efficient alternative to the filtered-x LMS and multiple error LMS algorithms (2002)

The Filtered-x LMS algorithm is currently the most popular method for adapting a filter when there exists a transfer function in the error path. Such instances arise, for example, in active control of sound and vibration. For multiple-input-multiple-output systems the Multiple Error LMS Algorithm is a generalization of Filtered-x LMS. The derivation of both algorithms rely on several assumptions, including linearity of the adaptive filter and error channel. Furthermore, in the Multiple Error LMS Algorithm the desirable order N computational complexity of LMS is lost, resulting in a prohibitive cost in certain DSP implementations. In this paper, we describe a new algorithm termed adjoint LMS which provides a simple alternative to the previously mentioned algorithms. In adjoint LMS, the error (rather than the input) is filtered through an adjoint filter of the error channel. Performance regarding convergence and misadjustment are equivalent. However, linearity is not assumed in the derivation of the algorithm. Furthermore, equations for single-input-single-output (SISO) and multiple-input-multiple-output (MIMO) are identical and both remain order N.
Role of Epithelial-Stem Cell Interactions during Dental Cell Differentiation (2012)

Epithelial-mesenchymal interactions regulate the growth and morphogenesis of ectodermal organs such as teeth. Dental pulp stem cells (DPSCs) are a part of dental mesenchyme, derived from the cranial neural crest, and differentiate into dentin forming odontoblasts. However, the interactions between DPSCs and epithelium have not been clearly elucidated. In this study, we established a mouse dental pulp stem cell line (SP) comprised of enriched side population cells that displayed a multipotent capacity to differentiate into odontogenic, osteogenic, adipogenic, and neurogenic cells. We also analyzed the interactions between SP cells and cells from the rat dental epithelial SF2 line. When cultured with SF2 cells, SP cells differentiated into odontoblasts that expressed dentin sialophosphoprotein. This differentiation was regulated by BMP2 and BMP4, and inhibited by the BMP antagonist Noggin. We also found that mouse iPS cells cultured with mitomycin C-treated SF2-24 cells displayed an epithelial cell-like morphology. Those cells expressed the epithelial cell markers p63 and cytokeratin-14, and the ameloblast markers ameloblastin and enamelin, whereas they did not express the endodermal cell marker Gata6 or mesodermal cell marker brachyury. This is the first report of differentiation of iPS cells into ameloblasts via interactions with dental epithelium. Co-culturing with dental epithelial cells appears to induce stem cell differentiation that favors an odontogenic cell fate, which may be a useful approach for tooth bioengineering strategies.
Timing Recovery for IEEE 1588 Applications in Telecommunications (2009)

IEEE 1588 is being proposed as a solution to transport synchronization information within telecommunication networks, both for infrastructure use and for use in applications where time and frequency are required. The requirements of synchronization in these cases, however, are quite different from the traditional use of IEEE 1588 for synchronization in control and instrumentation applications. For successful deployment in communication applications, it is necessary to consider the unique aspects of telecom sync and the interrelation of these factors with timing recovery using IEEE 1588. This paper discusses the error considerations that must be applied toward timing recovery in packet-timing implementations, particularly those targeted toward telecom sync. A review of basic rate-recovery concepts is used to identify the various sources of error and their effects. Simulation and experimental results are used to illustrate the impact of different factors on the recovered timing.
A unified mathematical model to quantify performance impairment for both chronic sleep restriction and total sleep deprivation (2013)

Performance prediction models based on the classical two-process model of sleep regulation are reasonably effective at predicting alertness and neurocognitive performance during total sleep deprivation (TSD). However, during sleep restriction (partial sleep loss) performance predictions based on such models have been found to be less accurate. Because most modern operational environments are predominantly characterized by chronic sleep restriction (CSR) rather than by episodic TSD, the practical utility of this class of models has been limited. To better quantify performance during both CSR and TSD, we developed a unified mathematical model that incorporates extant sleep debt as a function of a known sleep/wake history, with recent history exerting greater influence. This incorporation of sleep/wake history into the classical two-process model captures an individual's capacity to recover during sleep as a function of sleep debt and naturally bridges the continuum from CSR to TSD by reducing to the classical two-process model in the case of TSD. We validated the proposed unified model using psychomotor vigilance task data from three prior studies involving TSD, CSR, and sleep extension. We compared and contrasted the fits, within-study predictions, and across-study predictions from the unified model against predictions generated by two previously published models, and found that the unified model more accurately represented multiple experimental studies and consistently predicted sleep restriction scenarios better than the existing models. In addition, we found that the model parameters obtained by fitting TSD data could be used to predict performance in other sleep restriction scenarios for the same study populations, and vice versa. Furthermore, this model better accounted for the relatively slow recovery process that is known to characterize CSR, as well as the enhanced performance that has been shown to result from sleep banking.
Manganese Oxide Thin Film Preparation by Potentiostatic Electrolyses and Electrochromism (2000)

Manganese oxide thin films were deposited on transparent conducting tin oxide glass substrates by potentiostatic anodic electrolysis of alkaline solution of a manganese ammine complex at 298 K. The effects of varying deposition potentials on the microstructure and the electrochromic (EC) properties of the films were investigated. Characterization of films by X‐ray diffraction revealed that two distinct potential regions (lower and higher than 0.3 V vs. Ag/AgCl) were available for the film deposition; the crystal structure of the film deposited at lower and higher regions were and/or and , respectively. X‐ray photoelectron spectroscopy (XPS) analyses of the films featuring exchange splitting effect on Mn 3s spectra indicated that the valence of manganese in the films prepared at lower and higher potential regions are mixtures of divalence‐trivalence and of trivalence‐tetravalence, respectively. The XPS analysis also revealed that terminal chemical bonding species of the films are a mixture of hydroxide (Mn‐O‐H) and oxide (Mn‐O‐Mn). The mechanism of the EC process, by which the color change between brown and light yellow occurs, could be explained in terms of the transformation between these two oxygen groups in Mn‐O‐H and Mn‐O‐Mn, accompanied by the change in valence of Mn. The EC durability of the films in switching performance was also assessed. © 2000 The Electrochemical Society. All rights reserved.
Identification of <i>PCDH1</i> as a Novel Susceptibility Gene for Bronchial Hyperresponsiveness (2009)

PCDH1 is a novel gene for BHR in adults and children. The identification of PCDH1 as a BHR susceptibility gene may suggest that a structural defect in the integrity of the airway epithelium, the first line of defense against inhaled substances, contributes to the development of BHR.
Broadband waveguide-based spatial combiners (2002)

An array of tapered slotlines is inserted between rectangular waveguides. Results for a 2/spl times/4 active array at X-band are presented, indicating good combining efficiency and thermal properties as well as excellent bandwidth. To increase the device packing density and remove the lower cutoff frequency of the rectangular waveguide a coaxial combiner is also proposed. A radial arrangement of tapered slotlines is placed between two flared coaxial lines. 64 elements are combined with low combining loss over the 5 to 20 GHz band.
Effect of macerate enzymes on the yield, quality, volatile compounds and rheological property of prickly pear juice (2002)

Pectinase and cellulase enzymes were used to investigate efficacy for improving juice yield, stability and quality from prickly pear fruit. Pectinase improved the yield, stable color, color-assayed as release of anthocyanins or carotinoids and clarity of the juice. A significant increase in the effectiveness of pectinase was observed as the concentration was increased from 0.05 to 0.50% v/w. However, at concentration > 0.25% v/w they tended to impart a bitter flavor in the juice. Among three concentrations of pectinase and cellulase, pectinase at 0.50% v/w produced higher yield, a sediment-free clear juice and high-quality juice. The results indicated that depectinated clarified prickly pear juice behaves as a Newtonian fluid. It was found that the activation energy (Ea) for viscous flow was in the range of 5.02 x 10(3)-20.06 x 10(3) kJ/mol depending on the concentrations of pectinase and cellulase enzyme treatment of prickly pear juice, in contrast to 22.15 x 10(3) kJ/mol in untreated juice. Volatile compound concentrations of twelve compounds were not affected by pectinase and cellulase treatment. Overall the quality of prickly pear juice was better in pectinase-treated juice compared with untreated and cellulase-treated juice.
Improved detection of strong nonhomogeneities for STAP via projection statistics (2005)

In this paper, a robust statistical method called projection statistics (PS) is developed into an TV-dimensional complex form required to detect strong nonhomogeneities, or TV-dimensional outlier vectors (i.e., outliers), in space-time adaptive processing (STAP) radar training data, where N is the number of degrees of freedom (DOF) associated with the STAP scenario. The PS technique does not require the estimation of a covariance matrix, as is the case with the generalized inner product (GIP) test. Rather, it uses robust estimators of location (the median) and scale (the median absolute deviation from the median (MAD)) to identify and then excise strong nonhomogeneities in STAP training data that are capable of rendering traditional nonhomogeneity detection (NHD) techniques, such as the GIP, ineffective. Shown in the results section of this paper is sample matrix inversion (SMI) STAP performance using three separate NHD techniques: 1) a power test for high power outlier snapshots, 2) a GIP test, and 3) a PS-based test. The performance when not using any form of NHD is shown for comparison. It is clear from the results that the PS NHD method provides an SMI-STAP processor the best training data selection in the multi channel airborne radar measurements (MCARM) [B.N.S Babu et al., 1996] based radar data scenario studied here, and does so across all range cells of the scenario.
Apocynin inhibits peroxynitrite formation by murine macrophages (2000)

Peroxynitrite (ONOO(-)) the highly reactive coupling product of nitric oxide and superoxide, has been implicated in the pathogenesis of an increasing number of (inflammatory) diseases. At present, however, selective peroxynitrite antagonizing agents with therapeutic potential are not available. Therefore, the NADPH-oxidase inhibitor apocynin (4-hydroxy-3-methoxy-acetophenone) was tested for its ability to inhibit peroxynitrite formation in vitro The murine macrophage cell-line J774A.1, stimulated with IFNgamma/LPS, was used as a model. Conversion of 123-dihydrorhodamine (123-DHR) to its oxidation product 123-rhodamine was used to measure peroxynitrite production. Stimulated peroxynitrite formation could be completely inhibited by apocynin, by the superoxide scavenger TEMPO as well as by the nitric oxide synthase inhibitor aminoguanidine. Apocynin and aminoguanidine specifically inhibited superoxide and nitric oxide formation respectively as confirmed by measuring lucigenin enhanced chemiluminescence and nitrite accumulation. It is concluded that J774A.1 macrophages produce significant amounts of peroxynitrite, which is associated with nitric oxide production and NADPH-oxidase dependent superoxide formation. The NADPH-oxidase inhibitor apocynin proved to be a potent inhibitor of both superoxide and peroxynitrite formation by macrophages, which may be of future therapeutic significance in a wide range of inflammatory disorders.
Iatrogenic Creutzfeldt‐Jakob disease (1994)

We tested DNA from 15 centrally infected cases of iatrogenic Creutzfeldt-Jakob disease (CJD) (dura mater or corneal homografts and stereotactic EEG electrodes), 11 peripherally infected cases (native human growth hormone or gonadotrophin), and 110 control individuals for the presence of mutations in the chromosome 20 amyloid gene. No patient or control had any of the known pathogenic point or insert mutations found in familial disease, but allelic homozygosity at polymorphic codon 129 was present in all but two (92%) of the 26 patients, compared with 54 (50%) of the 110 controls (p < 0.001). Pooled data from all identified and tested cases of iatrogenic disease yielded a worldwide total of 56 patients, of whom all but four were homozygous at codon 129 (p < 0.001). These findings support the thesis that homozygosity at codon 129 enhances susceptibility to iatrogenic infections of both central and peripheral origin, with evident implications for the population of dura mater homograft and pituitary hormone recipients whose lives have been complicated by the possibility of exposure to the infectious agent of CJD.
Pressure tuned laser diode as a light source for characterization of optical transmission lines (2002)

Room-temperature emission of 1310 nm InGaAsP-InP laser diode has been studied as a function of hydrostatic pressure in an optical clamp cell up to 20 kbar. The diode was a commercial single-mode device by Siemens. The emission line shifted towards the shorter wavelengths similar to the bandgap of the constituent materials. The tuning range was over 200 nm. Here we consider the possibility of applying the high pressure cell with commercial laser diode inside as a pressure-tuned light source because of its narrow lines, wide tuning range and easy to implement portable equipment. It could be useful for measuring absorption and dispersion in optical transmission lines. Application of different types of electronically tunable lasers in the cell could further extend the tuning range and provide fine tuning and continuous tuning.
Digital Alloys of AlN/AlGaN for Deep UV Light Emitting Diodes (2005)

We report a systematic study of the optical and electrical properties of deep ultraviolet light emitting diodes based on digital alloy structures of AlN/Al 0.08 Ga 0.92 N grown by gas source molecular beam epitaxy with ammonia. Digital alloys are formed by short period superlattices consisting of Al 0.08 Ga 0.92 N wells, 0.50 or 0.75 nm thick, and AlN barriers, 0.75 to 1.5 nm thick. For digital alloys with effective bandgap of 5.1 eV, average AlN composition 72%, we obtain room temperature electron concentrations up to 1×10 19 cm -3 and resistivity of 0.005 Ω·cm and hole concentrations of 1×10 18 cm -3 with resistivity of 6 Ω·cm. Light emitting diodes based on digital alloys are demonstrated operating in the range of 250 to 290 nm.
Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics (1986)

Methodes d'extraction d'enzymes, d'electrophorese en gel et de coloration specifique des enzymes utilisees pour etudier la variation genetique chez Escherichia coli et d'autres bacteries. Les procedures decrites peuvent s'appliquer, avec quelques modifications mineures, a toute espece de bacterie
Monoclonal Antibodies to Sweet Taste Proteins. I. Analysis of Antigenic Epitopes on Thaumatin by Competitive Inhibition Assays (1991)

Using a competitive inhibition binding immunoassay, we have examined some of the antigenic epitopes present on thaumatin, an intense sweet tasting protein from the African fruit katemfe. We have developed a library of monoclonal antibodies which react with different surface antigenic epitopes on thaumatin. Some of these monoclonal antibodies also cross-react with monellin, another unrelated sweet tasting protein. The competitive binding immunoassay examines the immunoreactivity of both solid-phase and liquid-phase monoclonal antibodies. At least six major antigenic epitopes on thaumatin were identified by our library of monoclonal antibodies. This type of competitive binding analysis may prove useful in the discovery of "sweet taste determinants" on plant proteins and in the development of tandem immunoassays for quantitation of sweet tasting proteins in plant extracts.