gebhart/patrepeval huggingface dataset:
Mutational pattern of the nurse shark antigen receptor gene (NAR) is similar to that of mammalian Ig genes and to spontaneous mutations in evolution: the translesion synthesis model of somatic hypermutation (1999)

The pattern of somatic mutations of shark and frog Ig is distinct from somatic hypermutation of Ig in mammals in that there is a bias to mutate GC base pairs and a low frequency of mutations. Previous analysis of the new antigen receptor gene in nurse sharks (NAR), however, revealed no bias to mutate GC base pairs and the frequency of mutation was comparable to that of mammalian IgG. Here, we analyzed 1023 mutations in NAR and found no targeting of the mechanism to any particular nucleotide but did obtain strong evidence for a transition bias and for strand polarity. As seen for all species studied to date, the serine codon AGC/T in NAR was a mutational hotspot. The NAR mutational pattern is most similar to that of mammalian IgG and furthermore both are strikingly akin to mutations acquired during the neutral evolution of nuclear pseudogenes, suggesting that a similar mechanism is at work for both processes. In yeast, most spontaneous mutations are introduced by the translesion synthesis DNA polymerase zeta (REV3) and in various DNA repair-deficient backgrounds transitions were more often REV3-dependent than were transversions. Therefore, we propose a model of somatic hypermutation where DNA polymerase zeta is recruited to the Ig locus. An excess of DNA glycosylases in germinal center reactions may further enhance the mutation frequency by a REV3-dependent mutagenic process known as imbalanced base excision repair.
A screen-printed endotoxin sensor based on amperometry using a novel p-aminophenol conjugated substrate for a Limulus amebocyte lysate protease reaction (2013)

We developed a novel protease detection method based on amperometry using a p-aminophenol (pAP) conjugated substrate. We prepared Boc-Leu-Gly-Arg-pAP (LGR-pAP) as a novel substrate for a clotting enzyme, which is a protease activated by an endotoxin-induced Limulus amebocyte lysate (LAL) cascade reaction. The basic study using cyclic voltammetry revealed that the oxidation peak potentials of LGR-pAP and pAP were sufficiently separated from each other (0.25 V) to conduct amperometric detection of protease activity. We combined simple amperometric detection with a screen-printed electrode chip to produce a practical protease sensor. As an application of the sensor, we demonstrated quantitative endotoxin sensing. The endotoxin activated zymogens contained in the LAL to generate pAP, which was then electrochemically detected by potential step chronoamperometry (PSCA). The observed oxidation current increased with the concentration of endotoxin in the LAL assay solution. This PSCA detection was performed with a disposable chip sensor consisting of a screen-printed electrode and a fluidic channel with a hydrophilic cover. This chip sensor successfully detected 10-1000 EU L(-1) endotoxin within 60 min. This novel amperometric measurement with a screen-printed electrode not only provides compact, low-cost, and easy-to-use sensors for on-site monitoring of endotoxin, but also shows promise for use in other in vitro protease assays for biochemical research, diagnosis, and drug development.
Supercritical fluid injection of high‐molecular‐weight polycyclic aromatic compounds in capillary supercritical fluid chromatography (1986)

Abstract A sample introduction system for capillary supercritical fluid chromatography, which allows the dissolution of the sample in the supercritical mobile phase before being introduced into the column, was constructed and evaluated. Supercritical n ‐pentane was shown to solvate high‐molecular‐weight polycyclic aromatic compounds that could not be solvated using typical liquid solvents. In addition, split injection of a supercritical fluid solution was found to be more reproducible than split injections of a liquid solution. The potential of such an injection system was demonstrated, although further developments are needed in order to make the technique of practically utility.
Glutathione conjugation of chlorambucil: measurement and modulation by plant polyphenols (1997)

Chlorambucil (CMB), an anticancer drug, was cytotoxic at concentrations of 5-20 microM to human colon adenocarcinoma cells. It inhibited [14C]thymidine uptake in a dose-dependent manner. Both effects were potentiated by simultaneous exposure of the cells to 10 microM plant polyphenols. In an attempt to explain the possible mechanism of action of the polyphenols in relation to these observations, an HPLC-radiometric method was developed to measure the conjugation of CMB with glutathione in these cells and to monitor the export of monochloromonoglutathionyl CMB (MG-CMB), its main glutathione conjugate. At micromolar concentrations, five polyphenols, namely quercetin, butein, tannic acid, 2'-hydroxychalcone and morin, inhibited the efflux of CMB significantly; an inhibition of 40% was observed with 10 microM quercetin. The glutathione S-transferase (GST) activity of the cancer cells, measured with 1-chloro-2,4-dinitrobenzene, was also inhibited by the polyphenols. Their combined action on GST and on the efflux of MG-CMB conjugate could provide an enhanced positive modulation of sensitivity of the tumour cells to CMB.
Synthesis, Structure, and Acid−Base and Redox Properties of a Family of New Ru(II) Isomeric Complexes Containing the Trpy and the Dinucleating Hbpp Ligands (2003)

Three pairs of mononuclear geometrical isomers containing the ligand 3,5-bis(2-pyridyl)pyrazole (Hbpp) of general formula in- and out-[RuII(Hbpp)(trpy)X](n+) (trpy=2,2':6',2' '-terpyridine; X=Cl, n=1, 2a,b; X=H2O, n=2, 3a,b; X=py (pyridine), n=2, 4a,b) have been prepared through two different synthetic routes, isolated, and structurally characterized. The solid state structural characterization was performed by X-ray diffraction analysis of four complexes: 2a-4a and 4b. The structural characterization in solution was performed by means of 1D and 2D NMR spectroscopy for complexes 2a,b and 4a,b and coincides with the structures found in the solid state. All complexes were also spectroscopically characterized by UV-vis which also allowed us to carry out spectrophotometric acid-base titrations. Thus, a number of species were spectroscopically characterized with the same oxidation state but with a different degree of protonation. As an example, for 3a three pKa values were obtained: pKa1(RuII)=2.13, pKa2(RuII)=6.88, and pKa3(RuII)=11.09. The redox properties were also studied, giving in all cases a number of electron transfers coupled to proton transfers. The pH dependency of the redox potentials allowed us to calculate the pKa of the complexes in the Ru(III) oxidation state. For complex 3a, these were found to be pKa1(RuIII)=0.01, pKa2(RuIII)=2.78, and pKa3(RuIII)=5.43. The oxidation state Ru(IV) was only reached from the Ru-OH2 type of complexes 3a or 3b. It has also been shown that the RuIV=O species derived from 3a is capable of electrocatalytically oxidizing benzyl alcohol with a second-order rate constant of kcat=17.1 M(-1) s(-1).
Topical Antiglaucoma Treatment With Prostaglandin Analogues May Precipitate Meibomian Gland Disease (2010)

The popular use of topical prostaglandin analogues (TPAs) in glaucoma patients has a well-documented association with changes in number, length, thickness, and growth pattern of eyelashes. A link between meibomian gland disease and seborrheic blepharitis resulting from topical administration of prostaglandin analogues has not been made to the authors' knowledge. A retrospective study of 43 patients presenting for incision and curettage of chalazion to the operating theatre over a 2-year period was performed. The patients' histories were reviewed to establish whether they were using TPAs for glaucoma at the time of surgery. Eight patients (19%) were using TPAs at the time of surgery. No patient had a history of eyelid margin disease before the commencement of TPAs. The exact pathogenesis of how meibomian gland disease is linked to TPA use is unclear. We postulate that the topical application of prostaglandin analogues may contribute to the formation of chalazion by acting directly to stimulate meibomian gland secretion. The authors present the findings of a preliminary case series showing a higher-than-expected incidence of patients using TPAs requiring surgical intervention for chalazion.
The effect of anabolic steroids on ameliorating the adverse effects of chronic corticosteroids on intestinal anastomotic healing in rabbits. (1993)

The ability of anabolic steroids to reverse the deleterious effects of chronic corticosteroids on intestinal anastomotic healing was studied in 32 rabbits. Rabbits received once daily intramuscular injections of saline solution (controls), 0.1 milligram per kilogram of dexamethasone, dexamethasone plus low-dose anabolic steroids (2 milligrams per kilogram of nandrolone decanoate) or dexamethasone plus high-dose anabolic steroid (10 milligrams per kilogram of nandrolone decanoate). Two weeks later, the rabbits underwent small intestinal (SI) or large intestinal (LI) anastomoses. Postoperatively, injections were continued as before with the addition of high-dose anabolic steroids (20 milligrams per kilogram of nandrolone decanoate) to one-half of the dexamethasone only group. Seven days postoperatively, the rabbits had in situ assessment of anastomotic bursting pressure (ABP) and histologic examination using a modified Ehrlich/Hunt scale. Results demonstrated that dexamethasone severely impaired the healing of SI and LI anastomoses, with decreases in ABP to almost 50 percent of normal values and with similar reduction in histologic parameters. High-dose anabolic steroids reversed this inhibitory effect when initiated preoperatively or post-operatively, with increase in ABP to 72 percent of normal for SI anastomoses and 83 percent of normal for LI. No adverse effect of anabolic steroid administration was evident.
Simplified and automatic one‐pot synthesis of 16<i>α</i>‐[<sup>18</sup>F]fluoroestradiol without high‐performance liquid chromatography purification (2011)

16α‐[ 18 F]Fluoroestradiol (16α‐[ 18 F]FES, 1) is known as a valuable tracer in molecular imaging as estrogen receptor (ER) ligand for investigation of primary and metastatic breast cancer. ER concentration in human breast tumor cells is a significant indicator for the degree of disease and is often monitored by immunoassays or in vitro ligand binding of a tumor biopsy sample. More preferable non‐invasive diagnosis is accessible using 16α‐[ 18 F]FES (1) as PET tracer. Our aim was to develop a reliable, easy‐to‐use, remotely controlled synthesis for non carrier added (n.c.a.) 16α‐[ 18 F]FES (1) by nucleophilic substitution using a disposable cassette for GE TRACERlab® MX FDG . Purification of the crude product using solid phase extraction (SPE) cartridges, Oasis® WAX, HLB Plus, Sep‐Pak® C18 and Light Alumina N, allows abandonment of an HPLC purifying system. Formulation of the final product is included in the automatic synthesis. The experimental conditions for this easy‐to‐use synthesis for routine production of 16α‐[ 18 F]FES (1) are given in detail. Within 75 min 16α‐[ 18 F]FES (1) is produced in typically 20% n.c.a., radiochemical yield (non decay corrected). Chemical and radiochemical purity is >95% and >99%, respectively. Copyright © 2011 John Wiley & Sons, Ltd.
Gamma Distribution Model in the Evaluation of Breast Cancer Through Diffusion‐Weighted MRI: A Preliminary Study (2018)

This work shows that the GD model provides information in addition to the ADC parameter, suggesting its potential in the diagnosis of breast lesions. Level of Evidence 2: Technical Efficacy Stage 2 J. Magn. Reson. Imaging 2019;50:230-238.
3D Augmented Reality Mobile Application Prototype: for Visual Planning Support (2012)

The aim of this thesis is to implement a prototype of a 3D Augmented Reality mobile application. Using 3D is becoming more and more common for professionals as well as users. A good example of that is Google Earth and its 3D view. Implementing a mobile application that takes advantage of 3D and Augmented Reality may be very useful for planning new constructions in both urban and non-urban areas allowing to visualize how the construction will be in the future and how it will interact with its surrounding environment. There is a great potential for such kind of applications. An example could be the modification of a certain area of a city; allowing the inhabitants of that city to preview the project and, hopefully, avoiding unnecessary conflicts related to that project. In non-urban areas this application is also very useful for helping decision making by visualizing, on site, how the project will be and its impact on the environment. In order to preview a future construction there is the need to have a 3D model. Therefore, a 3D format for that model is necessary. Since COLLADA is a 3D standard interexchange format it is used in this thesis. Together with COLLADA, the computer graphics imagery and gaming technology called OpenGL ES 2.0 is used. Using COLLADA and OpenGL ES 2.0 combined with the properties of the views ’ layers, the camera input, the sensors in the mobile device and the positioning technologies permit obtaining successful results displaying a 3D object in an Augmented Reality mobile prototype application. Interface elements are implemented as well in order to bring basic usability tools. The results show the advantages of combining technologies in a mobile device and the problems derived from the low accuracy in positioning in such kind of devices. Thus, ideas for improving the positioning accuracy are discussed as well.
The role of <i>Aeromonas salmonicida</i> extracellular products in the pathology of furunculosis (1981)

Abstract. The extracellular products (ECP) of Aeromonas salmonicida , prepared by the cellophane overlay method, are lethal to rainbow trout when administered parenterally. Sublethal doses when injected i.p. or i.m. are shown to reproduce all the lesions that have been described in the literature as being associated with furunculosis. In addition, meningitis may be an important feature of furunculosi s and is reproduced by injection of ECP. A serum factor, probably an α‐globulin, present in normal serum of rainbow trout, is capable of neutralizing the toxic effects of ECP. The pathology is discussed with reference to the proteolytic and leucocidal properties of the ECP and its effects upon the eosinophilic granular cells which are caused to disperse and degranulate with the possible release of histamine.
High-speed burst-mode transmission technologies for 10-Gbit/s-class PON systems (2008)

We have developed an ac-coupled burst-mode transceiver using baseline-wander common-mode-rejection techniques for 10-Gbit/s-class PON systems. The fabricated transceiver achieved 10.3125-Gbit/s operation with an ultra-fast response.
Model-Based Processing of Microcantilever Sensor Arrays (2006)

In this paper, we have developed a model-based processor (MBP) for a microcantilever-array sensor to detect target species in solution. We perform a proof-of-concept experiment, fit model parameters to the measured data and use them to develop a Gauss–Markov simulation. We then investigate two cases of interest, averaged deflection data and multichannel data. For this evaluation we extract model parameters via a model-based estimation, perform a Gauss–Markov simulation, design the optimal MBP and apply it to measured experimental data. The performance of the MBP in the multichannel case is evaluated by comparison to a “smoother” (averager) typically used for microcantilever signal analysis. It is shown that the MBP not only provides a significant gain <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$(sim 80~ dB)$</tex> in signal-to-noise ratio (SNR), but also consistently outperforms the smoother by 40–60 dB. Finally, we apply the processor to the smoothed experimental data and demonstrate its capability for chemical detection. The MBP performs quite well, apart from a correctable systematic bias error.1625
Laser‐induced plasma spectroscopy for plastic identification (2000)

Abstract The goal of this work was instant identification of post‐consumer plastics by laser induced plasma spectrometry (LIPS). LIP spectra from plastics in a 200–800 nm spectral window were compared with reference spectral libraries stored in a computer. The libraries consisted of representative spectra from different groups of recycled plastic samples. The plasma emission spectra of polyethylene terephthalate (PET), high density polyethylene (HDPE), polyvinyl chloride (PVC), low density polyethylene (LDPE), polypropylene (PP) and polystyrene (PS) were studied. Simple statistical correlation methods including linear and rank correlations were used. The probabilities of correct identification ranged from 0.8 to 1 with values close to unity for most of the polymers studied.
Regulation of Amelogenin Gene Expression (2007)

The amelogenins are found uniquely in enamel, where they constitute the predominant class of secreted matrix proteins and where they play a fundamental role in normal enamel formation. To better understand the high level of tissue-specific expression, we cloned the bovine X and Y chromosomal amelogenin genes and the murine amelogenin gene and determined the DNA sequences for the regions upstream of the transcription start sites. We observed segments of strong homology among species, and identified consensus sequences for the binding of various transcription factors, including the glucocorticoid receptor, AP1, RXR and p53. Although specific sis-elements conferring enhanced transcription have not yet been identified, elements have been localized that have silencing effect in non-ameloblast cells. Conserved sequences are likely to be involved in tissue-specific expression. Transgenic mouse studies have shown that 3.5 kb of upstream region is sufficient but 900 bp is insufficient for specific expression in vivo. Alternative splicing of the primary transcript is an effective mechanism for generating molecular heterogeneity. Amelogenin genes contain seven exons, and exons 3, 4, 5 and most of 6 can be deleted by alternative splicing. However, the pattern of exon splicing varies according to the species, and skipping of bovine exon 3 appears to be developmentally regulated. It will be important to determine whether the relative amounts of translation products differ among species as do the mRNAs, and to correlate the various protein structures with function. These findings also suggest that the regulation of amelogenin gene expression is complex and takes place at several levels.
Oxidised Starch Plus Activated Charcoal in the Nephrectomised Rat and Uraemic Man (1977)

Impetus for study of intestinal sorbents in the management of renal failure stems from the initial trials of Yatzidis and Giordano, who administered oral doses of activated charcoal (Yatzidis, 1964) and oxidised starch (oxystarch) (Giordano et al. 1971, 1972), respectively, to uraemic patients as adjunctive therapy. Evidence of in vitro binding of nitrogen-containing compounds has been provided for oxystarch by Meriwether and Kramer (1976) and for charcoal by Yatzidis (1964), offering encouragement in the quest for extraction of clinically important quantities of nitrogenous wastes by ingested sorbents. Summarised herein are the results of administering a combination of oxidised starch and charcoal to two rat models of renal failure as well as a limited trial in uraemic patients.
[1,2,5]Oxadiazolo[3,4-<i>b</i>]pyrazine-5,6-diamine Derivatives as Mitochondrial Uncouplers for the Potential Treatment of Nonalcoholic Steatohepatitis (2020)

Small molecule mitochondrial uncouplers are emerging as a new class of molecules for the treatment of nonalcoholic steatohepatitis. We utilized BAM15, a potent protonophore that uncouples the mitochondria without depolarizing the plasma membrane, as a lead compound for structure-activity profiling. Using oxygen consumption rate as an assay for determining uncoupling activity, changes on the 5- and 6-position of the oxadiazolopyrazine core were introduced. Our studies suggest that unsymmetrical aniline derivatives bearing electron withdrawing groups are preferred compared to the symmetrical counterparts. In addition, alkyl substituents are not tolerated, and the N-H proton of the aniline ring is responsible for the protonophore activity. In particular, compound <b>10b</b> had an EC<sub>50</sub> value of 190 nM in L6 myoblast cells. In an <i>in vivo</i> model of NASH, <b>10b</b> decreased liver triglyceride levels and showed improvement in fibrosis, inflammation, and plasma ALT. Taken together, our studies indicate that mitochondrial uncouplers have potential for the treatment of NASH.
Prevention of acute graft-versus-host disease by magnetic nanoparticles of Fe3O4 combined with cyclosporin A in murine models (2011)

Taken together, Fe(3)O(4) MNPs may be used as a carrier of immunosuppressive agents to alleviate GVHD after allo-HSCT in murine models.
The essence of sustainable wealth creation : opinion (2010)

The redistribution of has been a major focus in South Africa in recent decades. There is an unstated assumption that wealth creates itself and is unjustly appropriated by certain individuals at the expense of those who justly deserve to participate in that wealth.
Engineering Drawing and Graphic Technology (1972)

1 Introduction2 Graphic Instruments and Their Use3 Constructional Geometry4 Basics of Lettering5 Orthographic Drawing and Sketching6 Sectional Views and Conventions7 Pictorial Drawing and Sketching8 Dimensioning and Tolerancing9 Lines and Planes in Space10 Surface Intersections11 Developed Views12 Introduction to Design13 Applications of Computer Aided Drafting14 Production Drawings15 Presenting Data: Charts and Graphs16 Assembly Elements: Threaded Fasteners, Keys, Springs17 Gears and Cams18 Welding and Riveting19 Electric and Electronic Drafting20 Piping21 Structural Drawing22 Maps and Topography
Using the Feature Exchange Language in the Next Generation Controller (1990)

Abstract : The Air Force has two ongoing initiatives to aid the ailing U.S. Machine Tool Industry. The first is the Intelligent Machining Workstation (IMW), which has the goal of automatically producing one-off quality parts. The second is a Next Generation Controller (NGC) initiative, which has as its primary objective to design and specify an open architecture controller for machine tools. This report analyzes whether the integration language developed for the IMW is adequate to support the requirements of an integration language needed to build the NGC. The IMW's Feature Exchange Language (FEL) is a simple message oriented language designed to integrate diverse modules. The NGC has a specified need to design a Neutral Manufacturing Language, which can be readily used to integrate diverse third-party modules into a coherent controller. We show how with a few minor extensions FEL can be used to meet this need.
Prebiotic Properties of Alternansucrase Maltose-Acceptor Oligosaccharides (2005)

alpha-(1-6) and alpha-(1-3)-linked oligosaccharides were obtained from the reaction between sucrose and maltose, catalyzed by an alternansucrase isolated from Leuconostoc mesenteroides NRRL B-21297 and separated using a Bio-Gel P2 column in six fractions. Fractions 1, 2, and 3 were mainly composed of DP3, DP4, and DP5, respectively. However, fractions 4, 5, and 6 consisted of mixtures from DP5 to DP9, and they are identified here as DP5.7, DP6.7, and DP7.4, respectively. Potential prebiotic properties of these oligosaccharides were tested using pure and mixed cultures. Generally, in pure studies, most of the tested bacteria failed to grow or grew poorly using the DP6.7 and DP7.4 fractions and showed the greatest growth on DP3. Growth of fecal bacteria on the maltose-acceptor products was tested following an in vitro fermentation method. DP3 showed the highest prebiotic effect, followed by DP4 and DP6.7, whereas DP7.4 did not present any prebiotic activity.