gebhart/patrepeval huggingface dataset:
Polydopamine/Liposome Coatings and Their Interaction with Myoblast Cells (2011)

Surface-mediated drug delivery is a recent concept, where active surface coatings are employed to deliver therapeutic cargo to cells. Herein, we explore the potential of liposomes embedded in polydopamine (PDA) coatings to serve as drug deposits stored on planar substrates. We quantify the PDA growth rate on glass by XPS and show that PDA coatings support myoblast adherence and proliferation. Further, PDA capping layers were deposited on glass substrates precoated with poly(L-lysine) and zwitterionic liposomes. Already thin PDA capping layers render liposome coated surfaces cell adhesive. We experimentally show for the first time, the internalization of a model hydrophobic cargo, that is, fluorescent lipids embedded within the lipid bilayer of liposomes by the cells from the surface. This is evident from the fluorescence exhibited by the cells grown on PDA coatings containing fluorescently labeled liposomes, with the highest fluorescent intensity found in the close proximity of the cell nuclei. The cargo uptake efficiency depends on the thickness of the PDA capping layer and the cell residence time on the coated substrates. Taken together, we demonstrate the first step toward the establishment of a versatile approach using liposomal drug deposits in polymer thin films for surface-mediated drug delivery.
The Optical Vortex Lens (2010)

The project seeks to fabricate a vortex lens (phase shift device) using optical lithography with a future goal of using it for astronomy purposes. initially the goal is to create a lens tuned to a HeNe laser (632.8 nm wavelength) with eight photoresist steps, which causes the light to undergo a phase shift. It is this shift that allows the vortex lens to suppress starlight in the vicinity of extra solar planets; it diffracts the light of the star while preserving the image of the nearby planet.
Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus (2012)

GW182 family proteins play important roles in microRNA (miRNA)-mediated gene silencing. They interact with Argonaute (Ago) proteins and localize in processing bodies, which are cytoplasmic foci involved in mRNA degradation and storage. Here, we demonstrated that human GW182 paralog, TNRC6A, is a nuclear-cytoplasmic shuttling protein, and its subcellular localization is conducted by a nuclear export signal (NES) and a nuclear localization signal (NLS) identified in this study. TNRC6A with mutations in its NES region was predominantly localized in the nucleus in an Ago-independent manner. However, it was found that TNRC6A could bring Ago protein into the nucleus via its Ago-interacting motif(s). Furthermore, miRNAs were also colocalized with nuclear TNRC6A-Ago and exhibited gene silencing activity. These results proposed the possibility that TNRC6A plays an important role in navigating Ago protein into the nucleus to lead miRNA-mediated gene silencing.
Chelating agents inhibit activity and prevent expression of streptococcal glucan-binding lectins (1992)

Several of the cariogenic mutans streptococci produce cell wall-associated glucan-binding lectins (GBLs). The lectins bind alpha-1,6-linked glucans and have no affinity for other polysaccharides or anomeric linkages. When citrate or lactate was included in the growth medium, expression of the activities of the GBLs of Streptococcus cricetus and S. sobrinus was prevented. Furthermore, chelating agents, including citrate, lactate, EDTA, and acetylacetone, were able to reversibly inhibit glucan-induced aggregation of GBL+ streptococci. In addition, the chelating agents prevented sucrose-dependent streptococcal adhesion to glass surfaces and dispersed preformed adherent masses of the streptococci. Neither citrate nor other chelating agents modified the activities of glucosyltransferases. Expression of the lectin could only be achieved by the addition of manganous ion to the growth medium. Chloramphenicol and other metabolic inhibitors prevented synthesis of GBL in cells obtained from manganese-deficient medium and shifted to manganous ion-sufficient medium. The GBL may be a manganoprotein, the manganese of which may be perturbed, but not removed, by chelating agents. During synthesis of the GBL, manganous ion may be required in order for the protein to achieve an active conformation. Citrate or other chelating agents may have promise as anticaries agents.
Corrosion Properties of Supersaturated Magnesium Alloy Systems (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Subaperture testing of aspheres with annular zones (1988)

A subaperture configuration to test aspheric optical components or systems has been developed. The aperture is divided into annular subaperture regions, and an interferometer is refocused for each region to reduce the fringe density. The method is suited to phase-measuring interferometry and may be used with standard interferogram reduction software. The theoretical basis for the method, requirements for the number of annular zones, sensitivity to the selection of the number of polynomials, and tolerances for centration and radius are described. Numerical and experimental validations of the algorithm are described. The method may eliminate the need to use null lenses for measuring some aspheres at the center of curvature.
Symbolic Path-Oriented Test Data Generation for Floating-Point Programs (2013)

Verifying critical numerical software involves the generation of test data for floating-point intensive programs. As the symbolic execution of floating-point computations presents significant difficulties, existing approaches usually resort to random or search-based test data generation. However, without symbolic reasoning, it is almost impossible to generate test inputs that execute many paths with floating-point computations. Moreover, constraint solvers over the reals or the rationals do not handle the rounding errors. In this paper, we present a new version of FPSE, a symbolic evaluator for C program paths, that specifically addresses this problem. The tool solves path conditions containing floating-point computations by using correct and precise projection functions. This version of the tool exploits an essential filtering property based on the representation of floating-point numbers that makes it suitable to generate path-oriented test inputs for complex paths characterized by floating-point intensive computations. The paper reviews the key implementation choices in FPSE and the labeling search heuristics we selected to maximize the benefits of enhanced filtering. Our experimental results show that FPSE can generate correct test inputs for selected paths containing several hundreds of iterations and thousands of executable floating-point statements on a standard machine: this is currently outside the scope of any other symbolic-execution test data generator tool.
Metrological evaluation of several Coriolis mass flowmeters (1992)

This paper is the final report of an evaluation and research project on the metrological performances of six different mass flowmeters of the Coriolis type which were extensively tested, under identical operating conditions, at the IMGC flow measurement laboratory, making use of the Italian national standard calibration facilities. The first results of the evaluation project, already detailed in a previous paper, are also briefly reviewed.
New strategies for drug discovery: activation of silent or weakly expressed microbial gene clusters (2012)

Genome sequencing of Streptomyces, myxobacteria, and fungi showed that although each strain contains genes that encode the enzymes to synthesize a plethora of potential secondary metabolites, only a fraction are expressed during fermentation. Interest has therefore grown in the activation of these cryptic pathways. We review current progress on this topic, describing concepts for activating silent genes, utilization of "natural" mutant-type RNA polymerases and rare earth elements, and the applicability of ribosome engineering to myxobacteria and fungi, the microbial groups known as excellent searching sources, as well as actinomycetes, for secondary metabolites.
A Simple Augmentation Method for Matchings with Applications to Streaming Algorithms (2018)

Given a graph G, it is well known that any maximal matching M in G is at least half the size of a maximum matching M^*. In this paper, we show that if G is bipartite, then running the Greedy matching algorithm on a sampled subgraph of G produces enough additional edges that can be used to augment M such that the resulting matching is of size at least (2 - sqrt{2})|M^*| ~~ 0.5857 |M^*| (ignoring lower order terms) with high probability. The main applications of our method lie in the area of data streaming algorithms, where an algorithm performs few passes over the edges of an n-vertex graph while maintaining a memory of size O(n polylog n). Our method immediately yields a very simple two-pass algorithm for Maximum Bipartite Matching (MBM) with approximation factor 0.5857, which only runs the Greedy matching algorithm in each pass. This slightly improves on the much more involved 0.583-approximation algorithm of Esfandiari et al. [ICDMW 2016]. To obtain our main result, we combine our method with a residual sparsity property of the random order Greedy algorithm and give a one-pass random order streaming algorithm for MBM with approximation factor 0.5395. This substantially improves upon the one-pass random order 0.505-approximation algorithm of Konrad et al. [APPROX 2012].
Role for DNA Damage Signaling in Pulmonary Arterial Hypertension (2013)

These results show for the first time that the DNA damage/PARP-1 signaling pathway is important for PAH development and provide a new therapeutic target for this deadly disease with high translational potential.
Improved electrothermal position sensing in MEMS with non-uniformly shaped heaters (2012)

For electrothermal MEMS position sensors, we introduce a non-uniform beam profile to enhance their sensitivity, linearity, and noise characteristics. Simulation results confirm that the proposed beam profile provides a more uniform temperature distribution compared to conventional electrothermal sensors with uniform beam cross sections. The effectiveness of the proposed sensor compared to a conventional design is experimentally verified by fabricating and testing an electrostatic SOI MEMS nanopositioner equipped with both types of sensors.
Gene expression in visceral and subcutaneous adipose tissues (2001)

A large body of evidence demonstrates depot-specific differences in the expression of genes coding important functional proteins in adipocytes. This may contribute to the well-known specific functional properties of the adipocytes from intra-abdominal and subcutaneous regions. This review will focus on the main findings regarding the regional differences in adipocyte gene expression in humans. These genes encode proteins belonging to three different functional groups: the metabolic enzyme and related signalling proteins, the adipogenic factors, and, finally, the products of adipocytes.
Determination of amatoxins and phallotoxins in <i>Amanita phalloides</i> mushrooms from northeastern Portugal by HPLC-DAD-MS (2015)

Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. α-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.
Efficacy and safety of rivastigmine in patients with Alzheimer's disease: international randomised controlled trial   Commentary: Another piece of the Alzheimer's jigsaw (1999)

Rivastigmine is well tolerated and effective. It improves cognition, participation in activities of daily living, and global evaluation ratings in patients with mild to moderately severe Alzheimer's disease. This is the first treatment to show compelling evidence of efficacy in a predominantly European population.
Chirp characteristics of dual-drive. Mach-Zehnder modulator with a finite DC extinction ratio (2002)

In this letter, we present theoretical and experimental studies on the chirp of the dual-drive Mach-Zehnder (MZ) modulator with a finite DC extinction ratio. The chirp of the modulator was measured using the fiber response peak method and compared with the theoretically calculated values. The results show that the residual chirp of the MZ modulator could be substantially reduced by driving the modulator in a push-pull mode with unequal amplitude signals.
A Guest-Responsive Fluorescent 3D Microporous Metal−Organic Framework Derived from a Long-Lifetime Pyrene Core (2010)

The carboxylate ligand 1,3,6,8-tetrakis(p-benzoic acid)pyrene (TBAPy)-based on the strongly fluorescent long-lifetime pyrene core-affords a permanently microporous fluorescent metal-organic framework, [In(2)(OH)(2)(TBAPy)].(guests) (1), displaying 54% total accessible volume and excellent thermal stability. Fluorescence studies reveal that both 1 and TBAPy display strong emission bands at 471 and 529 nm, respectively, upon excitation at 390 nm, with framework coordination of the TBAPy ligands significantly increasing the emission lifetime from 0.089 to 0.110 ms. Upon desolvation, the emission band for the framework is shifted to lower energy: however, upon re-exposure to DMF the as-made material is regenerated with reversible fluorescence behavior. Together with the lifetime, the emission intensity is strongly enhanced by spatial separation of the optically active ligand molecules within the MOF structure and is found to be dependent on the amount and chemical nature of the guest species in the pores. The quantum yield of the material is found to be 6.7% and, coupled with the fluorescence lifetime on the millisecond time scale, begins to approach the values observed for Eu(III)-cryptate-derived commercial sensors.
REACTION OF SUBSTITUTED BENZAMIDE OXIMES WITH CHLOROACETYL CHLORIDE AND THIOPHOSGENE (1999)

Abstract 3,4-Disubstituted-5,6-dihydro-4H-1,2,4-oxadiazine-5-ones were obtained from the reaction of substituted benzamide oximes with chloroacetyl chloride. The thionation of these compounds gave the corresponding 5-thiones. 3,4-Disubstituted-1,2,4-oxadiazole-5(4H)-thiones were obtained from the reaction of substituted benzamide oximes with thiophosgene. The copper-catalysed rearrangement of these compounds gave the isomeric 1,2,4-thiadiazole-5-ones.
Particulate Reinforcement of Polyacrylate Elastomers. I. Introduction and Preparation of Materials (1975)

Abstract The series of papers, of which this is the first, aims to demonstrate the importance of chemical reactions between filler particles and elastomer matrix in determining the physical properties of filled elastomers. A model system comprising small glass beads as the filler and crosslinked poly (ethyl acrylate) as the elastomer has been used. The beads themselves are inert towards the matrix during polymerization, but the reactivity of the surfaces of the beads can be varied by suitable treatments. This paper describes techniques for fractionating the beads to give narrow particle-size distribution, for preparing unfilled and filled elastomer sheets, and for preparing composites in which an elastomer sheet is polymerized in contact with a glass plate which may or may not have been previously subjected to surface treatment.
Detection of fault-prone software modules during a spiral life cycle (1996)

The article is an experience report on identifying fault prone modules in a subsystem of the Joint Surveillance Target Attack Radar System, JSTARS, a large tactical military system. The project followed the spiral life cycle model. The iterations of the system were developed in FORTRAN about one year apart. We developed a discriminant analysis model using software metrics from one iteration to predict whether or not each module in the next would be considered fault prone. Tactical military software is required to have high reliability. Each software function is often considered mission critical, and the lives of military personnel often depend on mission success. In our project, each iteration of a spiral life cycle development produced a system that was suitable for operational testing. A risk analysis based on operational testing guided development of the next iteration. Identifying fault prone modules early in the development of an iteration can lead to better reliability, The results confirm previously published studies that discriminant analysis can be a useful tool in identification of fault prone software modules. This study used consecutive iterations, first, to build, and then to evaluate the model. This model validation approach is more realistic than earlier studies which split data from one project to simulate two iterations. Model results could be used to identify those modules that would probably benefit from earlier reviews and testing, and thus, reduce the risk of unexpected problems with those modules.
Effects of Coumestrol on Estrogen Receptor Function and Uterine Growth in Ovariectomized Rats (1995)

Isoflavonoids and related compounds such as coumestrol have classically been categorized as phytoestrogens because these environmentally derived substances bind to the estrogen receptor (ER) and increase uterine wet weight in immature rats and mice. Assessment of the binding affinities of isoflavonoids for ER and subsequent effects on uterine growth suggest these compounds are less active estrogens than estradiol and therefore may reduce the risk of developing breast or prostate cancer in humans by preventing estradiol binding to ER. With the renewed interest in the relationships between environmental estrogens and cancer cause and prevention, we assessed the effects of the phytoestrogen coumestrol on uterotropic response in the immature, ovariectomized rat. Our studies demonstrated that in this animal model, coumestrol is an atypical estrogen that does not stimulate uterine cellular hyperplasia. Although acute (subcutaneous injection) or chronic (multiple injection or orally via drinking water) administration of coumestrol significantly increased uterine wet and dry weights, the phytoestrogen failed to increase uterine DNA content. The lack of true estrogenic activity was characterized by the inability of this phytoestrogen to cause cytosolic ER depletion, nuclear ER accumulation, or the stimulation of nuclear type II sites which characteristically precede estrogenic stimulation of cellular DNA synthesis and proliferation. In fact, subcutaneous or oral coumestrol treatment caused an atypical threefold induction of cytosolic ER without corresponding cytosolic depletion and nuclear accumulation of this receptor, and this increased the sensitivity of the uterus to subsequent stimulation by estradiol.(ABSTRACT TRUNCATED AT 250 WORDS)
CHARACTERIZATION OF LAMPREY FIBRINOPEPTIDES (1965)

1. Lamprey fibrinopeptide B is a relatively large peptide made up of about 40 amino acid residues. The peptide is highly electronegative, containing a large number of aspartic acid residues and a tyrosine O-sulphate residue. 2. The amino acid sequence of the first 18 residues from the N-terminal end of fibrinopeptide B has been established. The C-terminal ends with the sequence Val-Arg. Fibrino-peptide B is released by both lamprey and bovine thrombins. 3. Lamprey fibrino-peptide A is a short peptide containing only eight residues. The proposed amino acid sequence is: Asp-Asp-Ser-Ile/Leu-Asp-Ser-Leu/Ile-ArgThis peptide is released by lamprey thrombin but not by bovine thrombin.
High efficiency phosphorescent organic light emitting diodes using triplet quantum well structure (2007)

Light emission in phosphorescent quantum well structure which can confine excitons within an emitting layer was investigated. A multilayer quantum well structure which has a narrow triplet band gap host material sandwiched between wide triplet band gap host materials was designed, and device performances were studied. The multilayer emitting structure gave high efficiency of 47cd∕A compared with 11cd∕A of standard green devices.
Vascular endothelial growth factor and its correlation with angiogenesis and p53 expression in prostate cancer (2000)

BACKGROUND Previously it was demonstrated that in prostate tumors, angiogenesis measured as microvessel density (MVD) is associated with tumor stage as well as WHO grade and is an independent predictor of clinical outcome. Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. There is some evidence that P53 mutations cause overexpression of VEGF. We studied VEGF expression, p53 overexpression, and P53 mutations in prostate cancer (PCA) to investigate the role of VEGF as an angiogenic marker and the possible deregulation of VEGF as a result of P53 mutations in PCA. METHODS Immunohistochemical staining with a polyclonal VEGF antibody was performed in 55 paraffin-embedded PCA, in which MVD had previously been determined, as well as in 5 prostatic adenomas (PA) and 20 adjacent normal prostate tissues. In addition, 37 PCA and 5 PAs were examined for p53 expression by immunohistochemistry. Temperature gradient gel electrophoresis (TGGE) was performed in 13 of these PCA to screen for P53 mutations. VEGF expression, p53 expression, and mutations were then correlated with tumor stage, grade, MVD, and clinical outcome. RESULTS While PA and normal prostate tissue generally showed no or only low VEGF expression, there was a significant increase in VEGF expression with tumor stage, grade, and MVD in PCA. During clinical follow-up (mean, 31.9 months), 9 of 55 patients had tumor progression. Significant differences in VEGF expression were found between patients with tumor progression and those without (P = 0.0004). Of the 37 PCA evaluated for p53 expression, 12 exhibited p53 overexpression. TGGE revealed P53 mutations in 3 of 13 PCA. However, there was no correlation between VEGF expression, p53 overexpression, and P53 mutation, respectively. CONCLUSIONS VEGF seems to be an important, clinically relevant inducer of angiogenesis in PCA. VEGF expression was shown to correlate positively with tumor stage, grade, MVD, and clinical outcome. However, regulation of VEGF in PCA appears to be independent of p53 expression. Prostate 45:216–224, 2000. © 2000 Wiley-Liss, Inc.