gebhart/patrepeval huggingface dataset:
Suppression of tumorigenicity by the wild-type tuberous sclerosis 2 (Tsc2) gene and its C-terminal region. (1996)

The Tsc2 gene, which is mutationally inactivated in the germ line of some families with tuberous sclerosis, encodes a large, membrane-associated GTPase activating protein (GAP) designated tuberin. Studies of the Eker rat model of hereditary cancer strongly support the role of Tsc2 as a tumor suppressor gene. In this study, the biological activity of tuberin was assessed by expressing the wild-type Tsc2 gene in tumor cell lines lacking functional tuberin and also in rat fibroblasts with normal levels of endogenous tuberin. The colony forming efficiency of Eker rat-derived renal carcinoma cells was significantly reduced following reintroduction of wild-type Tsc2. Tumor cells expressing the transfected Tsc2 gene became more anchorage-dependent and lost their ability to form tumors in severe combined immunodeficient mice. At the cellular level, restoration of tuberin expression caused morphological changes characterized by enlargement of the cells and increased contact inhibition. As with the full-length Tsc2 gene, a clone encoding only the C terminus of tuberin (amino acids 1049-1809, including the GAP domain) was capable of reducing both colony formation and in vivo tumorigenicity when transfected into the Eker rat tumor cells. In normal Rat1 fibroblasts, conditional overexpression of tuberin also suppressed colony formation and cell growth in vitro. These results provide direct experimental evidence for the tumor suppressor function of Tsc2 and suggest that the tuberin C terminus plays an important role in this activity.
[Hypoxia regulates the expression of OPG/RANKL mRNA in rat bone marrow mesenchymal stem cells]. (2017)

Hypoxia can regulate the mRNA expression of OPG and RANKL mRNA in rBMSCs and significantly promote osteogenic differentiation.
Narrow-linewidth, singlemode erbium-doped fibrelaser with intracavity wave mixing in saturableabsorber (1994)

Narrow-linewidth, single longitudinal mode operation is demonstrated in an erbium-doped fibre laser with an intracavity nonlinear absorptive wave mixing of the counterpropagating beams. An unpumped length of erbium-doped fibre serves as the saturable absorber. Linewidths of the order of a few kilohertz are obtained.
Modulating emotional responses (2000)

Humans share with animals a primitive neural system for processing emotions such as fear and anger. Unlike other animals, humans have the unique ability to control and modulate instinctive emotional reactions through intellectual processes such as reasoning, rationalizing, and labeling our experiences. This study used functional MRI to identify the neural networks underlying this ability. Subjects either matched the affect of one of two faces to that of a simultaneously presented target face (a perceptual task) or identified the affect of a target face by choosing one of two simultaneously presented linguistic labels (an intellectual task). Matching angry or frightened expressions was associated with increased regional cerebral blood flow (rCBF) in the left and right amygdala, the brain's primary fear centers. Labeling these same expressions was associated with a diminished rCBF response in the amygdalae. This decrease correlated with a simultaneous increase in rCBF in the right prefrontal cortex, a neocortical region implicated in regulating emotional responses. These results provide evidence for a network in which higher regions attenuate emotional responses at the most fundamental levels in the brain and suggest a neural basis for modulating emotional experience through interpretation and labeling.
Synthesis and Antiproliferative Activities of 1-Substituted-3-(3-chloro-5-methoxyphenyl)-4-pyridinylpyrazole Derivatives Against Melanoma Cell Line (2009)

The synthesis of a new series of diarylureas and amides having a 1-substituted-3-(3-chloro-5-methoxyphenyl)-4- pyridinylpyrazole scaffold is reported here. The in vitro antiproliferative activities of these diaryl derivatives against human melanoma cell line A375 were tested and the effect of substituents on the phenyl ring was investigated. Most of the newly synthesized compounds generally showed superior or similiar activity against A375 to Sorafenib. Among these compounds, IId, IIg and IIh showed excellent activity against A375 compared to Sorafenib.
Acerca de los "Apuntes" y sus posibilidades en el estudio del teatro romántico español (1999)

Previous behavioural data indicate lower word-nonword recognition accuracy in association with a high level of positive schizotypy, psychopathy, or motor impulsivity traits, each with some unique contribution, in the general population. This study aimed to examine the neural underpinnings of these associations using functional magnetic resonance imaging (fMRI) in a volunteer sample. Twenty-two healthy English-speaking adults completed self-report measures of schizotypy (Oxford-Liverpool Inventory of Feelings and Experiences [O-LIFE]), psychopathy (Triarchic Psychopathy Measure [TriPM]), and impulsivity (Barratt Impulsiveness Scale [BIS-11]) and underwent whole-brain fMRI while performing a lexical decision task (LDT) featuring high and low-frequency words, real nonwords, and pseudohomophones. Higher positive schizotypy (Unusual Experiences) was associated with lower cerebellum activity during identification of low-frequency words (over real nonwords). Higher Boldness (fearless dominance) and Meanness (callous aggression) facets of psychopathy were associated with lower striatal and posterior cingulate activity when identifying nonwords over words. Higher Motor Impulsivity was associated with lower activity in the fusiform (bilaterally), inferior frontal (right-sided), and temporal gyri (bilaterally) across all stimuli-types over resting baseline. Positive schizotypy, psychopathy, and impulsivity traits influence word-nonword recognition through distinct neurocognitive mechanisms. Positive schizotypy and psychopathy appear to influence LDT performance through brain areas that play only a supportive (cerebellum) or indirect role in reading-related skills. The negative association between Motor Impulsivity and activations typically found for phonological processing and automatic word identification indicates a reduced bilateral integration of the meaning and sound of mental word representations, and inability to select the appropriate outputs, in impulsive individuals.
Kinetic measurements of photo-polymerization contraction in resins and composites (1991)

An instrument for the reproducible measurement of polymerization shrinkage kinetics is described, constructed around a disc-shaped specimen sandwiched between two glass plates. Test specimens of light-activated resins were irradiated through the lower, rigid plate. The physical response of this measurement system, in relation to the characteristics of photo-polymerized materials, has been subject to an original detailed analysis and validation. The upper non-rigid plate was readily deflected by an increase of the adhesive stress from the polymerizing and shrinking sample. Deflection was measured by a LVDT transducer and computer recorded. Dimensional changes were confined to the specimen disc-thickness dimension, such that the fractional linear shrinkage approximated the volumetric shrinkage. Shrinkage data are reported for representative unfilled and composite resins. Equilibrium shrinkage magnitudes ranged from 1.3 to 7.9%. The kinetic behaviour was approximately characterized by an overall time constant, ranging from 12.5-27 s, associated with an exponential growth curve, although the initial shrinkage was nearly linear in time, for many materials, due to non-steady-state concentrations of polymer free radicals.
Increasing protein O‐GlcNAc levels by inhibition of O‐GlcNAcase improves cardiac function following trauma hemorrhage and resuscitation in rat (2006)

Our previous studies have shown that administration of glucosamine during resuscitation following trauma‐hemorrhage (T‐H) improved cardiac output and organ perfusion. Glucosamine administration also increased levels of N‐acetyl‐glucosamine ( O ‐GlcNAc) on proteins in heart and brain. An alternative means of increasing O ‐GlcNAc levels is by inhibition of O ‐GlcNAcase, which catalyzes removal of GlcNAc from proteins with O ‐(2‐acetamido‐2‐deoxy‐d‐glucopyranosylidene) amino‐ N ‐phenylcarbamate (PUGNAc). The goal of this study therefore, was to determine whether PUGNAc administration following T‐H also improves recovery following T‐H. Fasted male rats were bled to and maintained at a mean arterial blood pressure of 40 mmHg for 90 min followed by fluid resuscitation. Administration of 300μ l of 20mM PUGNAc 30 min after the onset of resuscitation significantly improved cardiac output compared to the vehicle controls (14±2 vs. 26±2 mls/min/100g body wt; p<0.05); decreased total peripheral resistance (6.2±0.9 vs. 3.7±3 mmHg/mls/min/100g body wt; p<0.05), and increased perfusion of critical organs systems, including kidney, liver and brain, determined 2hrs after the end of resuscitation. PUGNAc also attenuated the T‐H induced increase in plasma IL‐6 levels (864±112 Vs 392±188 pmols/ml; p<0.05). PUGNAc also significantly increased O ‐GlcNAc levels in heart, liver and skeletal muscle but not brain. Thus, PUGNAc, like glucosamine, improves cardiac function and organ perfusion and reduced the level of circulating IL‐6 following T‐H. The similar effects of glucosamine and PUGNAc suggest that this protection is mediated via increased protein O ‐GlcNAc levels. Supported by NIH grants RO1 HL076165‐01(RBM) and R37 GM39519 (IHC).
Body mass index as a predictor of hepatic steatosis in living liver donors (2001)

Evaluation of the living donor for liver transplantation is a complex process involving such invasive studies as liver biopsy and angiography. It is important to establish the likelihood and extent of hepatic steatosis in living donors by clinical, imaging, and biochemical parameters to avoid performing a liver biopsy, if possible. In this study, the predictive value of body mass index (BMI), liver chemistry tests, and imaging studies was compared with liver histological examination in 33 potential living donors. Patients were grouped and compared based on their BMI (<25, 25 to 28, >28). No patient with a BMI less than 25 had hepatic steatosis. Of patients with a BMI of 25 to 28, steatosis was found on biopsy in 3 of 9 patients. Thirteen of 17 patients (76%) with a BMI greater than 28 had hepatic steatosis on liver biopsy. There was a significant correlation between BMI and overall grade of steatosis (R = 0.49). All subjects with steatosis detected on magnetic resonance imaging (MRI) or computed tomography (CT) had steatosis on biopsy, and all but 2 such patients had greater than 10% steatosis on biopsy. Conversely, 30% of patients in the MRI group and 24% of patients in the CT group failed to show hepatic steatosis when it was present on biopsy. Thus, it appears that liver biopsy could be avoided in subjects with a normal BMI and absence of risk factors. Individuals with a high BMI should undergo liver biopsy because biochemical and imaging data are currently inadequate to determine the extent of steatosis. Future studies should aim at improving the sensitivity of imaging techniques in the diagnosis of steatosis.
A conceptual review on polymer electrolytes and ion transport models (2018)

This review article provides a deep insight into the ion conduction mechanism in polymer electrolytes (PEs). The concepts of different categories of polymer electrolytes are discussed. The significance of the existence of functional (polar) groups on the backbone of host polymers, which are used in polymer electrolytes, is well explained. The working principle of electrical impedance spectroscopy (EIS) is overviewed. The relationship between impedance plots and equivalent circuits, which are crucial for electrical characterization, is extensively interpreted. Based on the patterns of dc conductivity (σdc) versus 1000/T, the ion transport models of Arrhenius and Vogel–Tammann–Fulcher (VTF) are discussed. Effects of coupling and decoupling between ionic motion and polymer segmental relaxation are analyzed. The important role of dielectric constant on cationic transport in PEs is also explained. The relationships existing between electrical and dielectric parameters are elucidated, which help interpret and understand the ion conduction mechanism. From the reported empirical curves of dc conductivity vs. dielectric constant, the reformulated Arrhenius [σdc(T)=σ0exp(−EakBTε′)] equation is proposed. Finally, other important phenomena, occurring in polymer electrolytes, are shown to be understandable from the dielectric constant studies.
Using the drug-protein interactome to identify anti-ageing compounds for humans (2019)

Advancing age is the dominant risk factor for most of the major killer diseases in developed countries. Hence, ameliorating the effects of ageing may prevent multiple diseases simultaneously. Drugs licensed for human use against specific diseases have proved to be effective in extending lifespan and healthspan in animal models, suggesting that there is scope for drug repurposing in humans. New bioinformatic methods to identify and prioritise potential anti-ageing compounds for humans are therefore of interest. In this study, we first used drug-protein interaction information, to rank 1,147 drugs by their likelihood of targeting ageing-related gene products in humans. Among 19 statistically significant drugs, 6 have already been shown to have pro-longevity properties in animal models (p < 0.001). Using the targets of each drug, we established their association with ageing at multiple levels of biological action including pathways, functions and protein interactions. Finally, combining all the data, we calculated a ranked list of drugs that identified tanespimycin, an inhibitor of HSP-90, as the top-ranked novel anti-ageing candidate. We experimentally validated the pro-longevity effect of tanespimycin through its HSP-90 target in Caenorhabditis elegans.
Investigation of the electrocaloric effect in a PbMg2/3Nb1/3O3-PbTiO3 relaxor thin film (2009)

Permittivity measurements of a 0.93PMN-0.07PT thin film show a broad maximum near 35 °C, and an anomaly at the depolarizing temperature Td=18 °C on heating only, suggesting a dipolar glass-relaxor phase transition. No structural phase transition at 18 °C is apparent from ferroelectric hysteresis loops taken on field cooling and field heating. These loops show the thermal hysteresis expected for ferroelectric relaxors, which has not hitherto been experimentally verified in PbMg2/3Nb1/3O3-PbTiO3 thin films. Our data suggest the intriguing possibility of a giant electrocaloric effect (ΔT=9 K, ΔE=720 kV cm−1) at and near room temperature.
Potential antiinflammatory effects of interleukin 4: suppression of human monocyte tumor necrosis factor alpha, interleukin 1, and prostaglandin E2. (1989)

Stimulated human monocytes/macrophages are a source of mediators such as tumor necrosis factor alpha (TNF-alpha), interleukin 1 (IL-1), and prostaglandin E2 (PGE2), which can modulate inflammatory and immune reactions. Therefore, the ability to control the production of such mediators by monocytes/macrophages may have therapeutic benefits, and it has been proposed that glucocorticoids may act in this way. Purified human monocytes, when stimulated in vitro with lipopolysaccharide (LPS) or with LPS and gamma interferon (IFN-gamma), produce TNF-alpha, IL-1, and PGE2. Cotreatment of stimulated cells with the purified human lymphokine, interleukin 4 (IL-4 greater than or equal to 0.1-0.5 unit/ml; 12-60 pM) dramatically blocked the increased levels of these three mediators; for TNF-alpha and IL-1, the inhibition was manifest at the level of mRNA. Thus, IL-4 can suppress some parameters of monocyte activation and, as for B cells, have opposite effects to IFN-gamma. The effects of IL-4 on human monocytes are similar to those obtained with the glucocorticoid dexamethasone (0.1 microM).
Anti-allodynic effect of NW-1029, a novel Na+ channel blocker, in experimental animal models of inflammatory and neuropathic pain (2003)

NW-1029, a benzylamino propanamide derivative, was selected among several molecules of this chemical class on the basis of its affinity for the [(3)H]batracotoxin ligand displacement of the Na(+) channel complex and also on the basis of its voltage and use-dependent inhibitory action on the Na(+) currents of the rat DRG (dorsal root ganglia) sensory neuron. This study evaluated the analgesic activity of NW-1029 in animal models of inflammatory and neuropathic pain (formalin test in mice, complete Freund's adjuvant and chronic constriction injury in rats) as well as in acute pain test (hot-plate and tail-flick in rats). Orally administered NW-1029 dose-dependently reduced cumulative licking time in the early and late phase of the formalin test (ED(50)=10.1 mg/kg in the late phase). In the CFA model, NW-1029 reversed mechanical allodynia (von Frey test) after both i.p. and p.o. administration (ED(50)=0.57 and 0.53 mg/kg), respectively. Similarly, NW-1029 reversed mechanical allodynia in the CCI model after both i.p. and p.o. administration yielding an ED(50) of 0.89 and 0.67 mg/kg, respectively. No effects were observed in the hot-plate and tail-flick tests up to 30 mg/kg p.o. The compound orally administered (0.1-10 mg/kg) was well tolerated, without signs of neurological impairment up to high doses (ED(50)=470 and 245 mg/kg in rat and mice Rotarod test, respectively). These results indicate that NW-1029 has anti-nociceptive properties in models of inflammatory and neuropathic pain.
Differential Thermal Analysis (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDifferential Thermal AnalysisC. B. MurphyCite this: Anal. Chem. 1962, 34, 5, 298R–301rPublication Date (Print):April 1, 1962Publication History Published online1 May 2002Published inissue 1 April 1962https://doi.org/10.1021/ac60185a031RIGHTS & PERMISSIONSArticle Views233Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (616 KB) Get e-Alerts Get e-Alerts
Characterization of adhesive epitopes with the OmpS display system (2000)

OmpS is an outer membrane protein of Vibrio cholerae where it forms trimeric pores that function in the uptake of maltose and maltodextrins. Based on sequence similarity to LamB proteins, a model of OmpS folding in the outer membrane has been constructed. According to this model, OmpS contains 18 transmembrane beta-strands and nine surface-accessible loops. Adhesive epitopes can, when inserted into surface-accessible loop 4 (L4) and expressed in Escherichia coli, retain their functional characteristics. We inserted three D-repeats from the Staphylococcus aureus fibronectin-binding protein FnBPA into L4 of OmpS and showed that E. coli cells expressing these hybrids bind fibronectin. DNA fragments covering the N-terminal half of the globoside-binding P-fimbrial adhesin class II PapG of E. coli were cloned into the same surface accessible loop (L4) of OmpS. Fragments of papG encoding 53 or 186 amino acids from the N-terminal end of class II PapG adhesin were found to confer bacterial adhesiveness to globoside. Removal of 23 amino acids from the N-terminus of PapG did not affect receptor binding, but removal of 31 amino acids abolished it. The newly developed night sky image technique was also used to demonstrate the binding properties of membrane vesicles carrying the hybrid proteins. We raised antibodies against the purified hybrid protein containing 53 amino acids from PapG. This antiserum recognized the P-fimbriae on E. coli cells. These data provide evidence that the N-terminal first 53 amino acids of class II PapG contain the receptor-binding domain.
Morbidity and mortality following early administration of amiodarone in acute myocardial infarction (2000)

This study demonstrated that early administration of amiodarone in low doses to patients with an acute myocardial infarction may be used only if life-threatening arrhythmia justify its prescription. Conversely, when given in high doses, it might increase mortality.
Optimized look‐ahead tree policies: a bridge between look‐ahead tree policies and direct policy search (2013)

SUMMARY Direct policy search (DPS) and look‐ahead tree (LT) policies are two popular techniques for solving difficult sequential decision‐making problems. They both are simple to implement, widely applicable without making strong assumptions on the structure of the problem, and capable of producing high‐performance control policies. However, computationally, both of them are, each in their own way, very expensive. DPS can require huge offline resources (effort required to obtain the policy) to first select an appropriate space of parameterized policies that works well for the targeted problem and then to determine the best values of the parameters via global optimization. LT policies do not require any offline resources; however, they typically require huge online resources (effort required to calculate the best decision at each step) in order to grow trees of sufficient depth. In this paper, we propose optimized LTs (OLTs), a model‐based policy learning scheme that lies at the intersection of DPS and LT. In OLT, the control policy is represented indirectly through an algorithm that at each decision step develops, as in LT by using a model of the dynamics, a small LT until a prespecified online budget is exhausted. Unlike LT, the development of the tree is not driven by a generic heuristic; rather, the heuristic is optimized for the target problem and implemented as a parameterized node scoring function learned offline via DPS. We experimentally compare OLT with pure DPS and pure LT variants on optimal control benchmark domains. The results show that the LT‐based representation is a versatile way of compactly representing policies in a DPS scheme (which results in OLT being easier to tune and having lower offline complexity than pure DPS) and at the same time DPS helps to significantly reduce the size of the LTs that are required to take high‐quality decisions (which results in OLT having lower online complexity than pure LT). Moreover, OLT produces overall better performing policies than pure DPS and pure LT, and also results in policies that are robust with respect to perturbations of the initial conditions. Copyright © 2013 John Wiley & Sons, Ltd.