gebhart/patrepeval huggingface dataset:
Theoretical modeling of yields for proton-induced reactions on natural and enriched molybdenum targets (2011)

Recent acute shortage of medical radioisotopes prompted investigations into alternative methods of production and the use of a cyclotron and ¹⁰⁰Mo(p,2n)(99m)Tc reaction has been considered. In this context, the production yields of (99m)Tc and various other radioactive and stable isotopes which will be created in the process have to be investigated, as these may affect the diagnostic outcome and radiation dosimetry in human studies. Reaction conditions (beam and target characteristics, and irradiation and cooling times) need to be optimized in order to maximize the amount of (99m)Tc and minimize impurities. Although ultimately careful experimental verification of these conditions must be performed, theoretical calculations can provide the initial guidance allowing for extensive investigations at little cost. We report the results of theoretically determined reaction yields for (99m)Tc and other radioactive isotopes created when natural and enriched molybdenum targets are irradiated by protons. The cross-section calculations were performed using a computer program EMPIRE for the proton energy range 6-30 MeV. A computer graphical user interface for automatic calculation of production yields taking into account various reaction channels leading to the same final product has been created. The proposed approach allows us to theoretically estimate the amount of (99m)Tc and its ratio relative to (99g)Tc and other radioisotopes which must be considered reaction contaminants, potentially contributing to additional patient dose in diagnostic studies.
Two worlds apart (2003)

A tension exists between designers' comfort with physical artifacts and the need for effective remote collaboration: physical objects live in one place. Previous research and technologies to support remote collaboration have focused on shared electronic media. Current technologies force distributed teams to choose between the physical tools they prefer and the electronic communication mechanisms available. We present Distributed Designers' Outpost, a remote collaboration system based on The Designers' Outpost, a collaborative web site design tool that employs physical Post-it notes as interaction primitives. We extended the system for synchronous remote collaboration and introduced two awareness mechanisms: transient ink input for gestures and a blue shadow of the remote collaborator for presence. We informally evaluated this system with six professional designers. Designers were excited by the prospect of physical remote collaboration but found some coordination challenges in the interaction with shared artifacts.
Mouse oocytes within germ cell cysts and primordial follicles contain a Balbiani body (2006)

The Balbiani body or mitochondrial cloud is a large distinctive organelle aggregate found in developing oocytes of many species, but its presence in the mouse has been controversial. Using confocal and electron microscopy, we report that a Balbiani body does arise in mouse neonatal germline cysts and oocytes of primordial follicles but disperses as follicles begin to grow. The mouse Balbiani body contains a core of Golgi elements surrounded by mitochondria and associated endoplasmic reticulum. Because of their stage specificity and perinuclear rather than spherical distribution, these clustered Balbiani body mitochondria may have been missed previously. The Balbiani body also contains Trailer hitch, a widely conserved member of a protein complex that associates with endoplasmic reticulum/Golgi-like vesicles and transports specific RNAs during Drosophila oogenesis. Our results provide evidence that mouse oocytes develop using molecular and developmental mechanisms widely conserved throughout the animal kingdom.
Characterization of a component of the yeast secretion machinery: identification of the SEC18 gene product. (1988)

SEC18 gene function is required for secretory protein transport between the endoplasmic reticulum (ER) and the Golgi complex. We cloned the SEC18 gene by complementation of the sec18-1 mutation. Gene disruption has shown that SEC18 is essential for yeast cell growth. Sequence analysis of the gene revealed a 2,271-base-pair open reading frame which could code for a protein of 83.9 kilodaltons. The predicted protein sequence showed no significant similarity to other known protein sequences. In vitro transcription and translation of SEC18 led to the synthesis of two proteins of approximately 84 and 82 kilodaltons. Antisera raised against a Sec18-beta-galactosidase fusion protein also detected two proteins (collectively referred to as Sec18p) in extracts of 35S-labeled yeast cells identical in size to those seen by in vitro translation. Mapping of the 5' end of the SEC18 mRNA revealed only one major start site for transcription, which indicates that the multiple forms of Sec18p do not arise from mRNAs with different 5' ends. Results of pulse-chase experiments indicated that the two forms of Sec18p are not the result of posttranslational processing. We suggest that translation initiating at different in-frame AUG start codons is likely to account for the presence of two forms of Sec18p. Hydrophobicity analysis indicated that the proteins were hydrophilic in nature and lacked any region that would be predicted to serve as a signal sequence or transmembrane anchor. Although potential sites for N-linked glycosylation were present in the Sec18p sequence, the sizes of the in vivo SEC18 gene products were unaffected by the drug tunicamycin, indicating that Sec18p does not enter the secretory pathway. These results suggest that Sec18p resides in the cell cytoplasm. While preliminary cell fractionation studies showed that Sec18p is not associated with the ER or Golgi complex, association with a 100,000 x g pellet fraction was observed. This suggests that Sec18p may bind transiently to small vesicles such as those presumed to participate in secretory protein transport between ER and the Golgi complex.
Study of mechano-chemical machining of ceramics and the effect on thin film behavior. Final technical report 1 May 80-30 Nov 82 (1983)

Efforts have been made in this program to develop mechanochemical polishing technique for a number of materials and to determine the consequences of mechanochemically polished surfaces on the physical performance of ceramics. The objective of the mechanochemical polishing efforts is to produce scratch-and-damage-free surfaces by finding suitable soft abrasives that remove material from the workpiece through chemical reactions. Mechanochemical polishing effects were observed when hot-pressed and reaction bonded SI3N4 were polished with two oxides of iron, Fe2O3 and Fe3O4. Mechanochemicaly polished surfaces of Si3N4 were analyzed using the techniques of Auger electro spectroscopy, profilometry and interferometry. Data showing the effects of mechanochemical polishing on the strength of hot-pressed Si3N4 and on the adhesion of thin titanium films on this material have been obtained. Also preliminary efforts have been made to find suitable soft abrasives for mechanochemical polishing of GaAs, Spinel (MgO.A12O3) Sic, B4C,and partially stabilized ZrO2.
Flow structures and particle deposition patterns in double-bifurcation airway models. Part 2. Aerosol transport and deposition (2001)

The flow theory and air flow structures in symmetric double-bifurcation airway models assuming steady laminar, incompressible flow, unaffected by the presence of aerosols, has been described in a companion paper (Part 1). The validated computer simulation results showed highly vortical flow fields, especially around the second bifurcations, indicating potentially complex particle distributions and deposition patterns. In this paper (Part 2), assuming spherical non-interacting aerosols that stick to the wall when touching the surface, the history of depositing particles is described. Specifically, the finite-volume code CFX (AEA Technology) with user-enhanced FORTRAN programs were validated with experimental data of particle deposition efficiencies as a function of the Stokes number for planar single and double bifurcations. The resulting deposition patterns, particle distributions, trajectories and time evolution were analysed in the light of the air flow structures for relatively low ( Re D 1 = 500) and high ( Re D 1 = 2000) Reynolds numbers and representative Stokes numbers, i.e. St D 1 = 0.04 and St D 1 = 0.12. Particle deposition patterns and surface concentrations are largely a function of the local Stokes number, but they also depend on the fluid–particle inlet conditions as well as airway geometry factors. While particles introduced at low inlet Reynolds numbers (e.g. Re D 1 = 500) follow the axial air flow, secondary and vortical flows become important at higher Reynolds numbers, causing the formation of particle-free zones near the tube centres and subsequently elevated particle concentrations near the walls. Sharp or mildly rounded carinal ridges have little effect on the deposition efficiencies but may influence local deposition patterns. In contrast, more drastic geometric changes to the basic double-bifurcation model, e.g. the 90°-non-planar configuration, alter both the aerosol wall distributions and surface concentrations considerably.
EUTECTIC MIXTURES OF DRUGS WITH POOR AQUEOUS SOLUBILITY - SOLID STATE CHARACTERIZATION AND DISSOLUTION STUDIES (2012)

It is a well reported fact that large number of drugs coming of the drug discovery pipeline show poor aqueous solubility. Eutectic mixture formation of poorly soluble drugs with hydrophilic carriers has been used to enhance the dissolution rate of such poorly soluble drugs. Eutectic mixtures are solid dispersions where the drug and the carrier are both in crystalline form. The eutectic mixture has a lower melting point than either component. Eutectic mixtures are thermodynamically stable systems. The feasibility of developing a dosage form from an eutectic mixture depends on the phase diagram. Poloxamers are polyoxyethylene-polyoxypropylene-polyoxyethylene block polymers which have surfactant properties. Phase diagram construction and dissolution rate enhancement mechanism in crystalline poloxamer based eutectics has not been reported in pharmaceutical literature. This thesis involved the detailed study of poloxamer 188 (PL 188) based eutectic mixtures. Eutectic mixture formation between PL 188 and drugs with diverse physicochemical properties was proved. Accurate experimental phase diagrams were constructed using solid state characterization techniques and theoretical phase diagrams were predicted using Lacoulonche et al's model. The model was accurate in predicting the phase diagrams of most drugs. Discrepancies were observed in case of drugs showing hydrogen bonding interactions with PL 188. This was confirmed by a blue shift of the carbonyl band using fourier transform infra-red spectroscopy. A unique novel graphical method for estimating the accurate eutectic composition of PL 188 based eutectics with about 50 mg of drug was devised. PL 188 was effective in improving the dissolution rate of a poorly soluble drug ibuprofen in pH 1, 4.5 and 7.2. For the first time a detailed study establishing melting point depression due to eutectic formation as a reason for dissolution enhancement was described. Contrary to expectation it was realized that maximum dissolution rate enhancement takes place at drug ratios well above the eutectic composition. The utility of PL 188 as a eutectic mixture carrier was shown by comparing ketoprofen PL 188 eutectic mixtures with ketoprofen soluplus (glass solutions) and ketoprofen urea solid dispersions(amorphous precipitation in crystalline carrier). The ketoprofen PL 188 eutectic mixtures had better dissolution enhancing effect and physical stability.
Synthetic Studies of Indoles and Related Compounds. XXVII. A New Synthesis of Crenatine from Ethyl Indole-2-carboxylate. (1991)

Crenatine (1a), which is a member of a new class of β-carboline alkaloids having an oxygen functionality at the 4-position, was synthesized starting from ethyl 1-benzylindole-2-carboxylate (12a) via cyclization of an elaborated C2-subsitituent to the 3-position of the indole nucleus and aluminum chloride-catalyzed debenzylation of the protected indolic nitrogen. 1-Ethyl-4-hydroxy-9-methyl-β-carboline (26b), a positional isomer of crenatine with regard to the methyl group, was also synthesized through the same methodology.
Chemokine Signatures in the Skin Disorders of Lyme Borreliosis in Europe: Predominance of CXCL9 and CXCL10 in Erythema Migrans and Acrodermatitis and CXCL13 in Lymphocytoma (2007)

The three skin disorders of Lyme borreliosis in Europe include erythema migrans, an acute, self-limited lesion; borrelial lymphocytoma, a subacute lesion; and acrodermatitis chronica atrophicans, a chronic lesion. Using quantitative reverse transcription-PCR, we determined mRNA expression of selected chemokines, cytokines, and leukocyte markers in skin samples from 100 patients with erythema migrans, borrelial lymphocytoma, or acrodermatitis chronica atrophicans and from 25 control subjects. Chemokine patterns in lesional skin in each of the three skin disorders included low but significant mRNA levels of the neutrophil chemoattractant CXCL1 and the dendritic cell chemoattractant CCL20 and intermediate levels of the macrophage chemoattractant CCL2. Erythema migrans and particularly acrodermatitis lesions had high mRNA expression of the T-cell-active chemokines CXCL9 and CXCL10 and low levels of the B-cell-active chemokine CXCL13, whereas lymphocytoma lesions had high levels of CXCL13 and lower levels of CXCL9 and CXCL10. This pattern of chemokine expression was consistent with leukocyte marker mRNA in lesional skin. Moreover, using immunohistologic methods, CD3(+) T cells and CXCL9 were visualized in erythema migrans and acrodermatitis lesions, and CD20(+) B cells and CXCL13 were seen in lymphocytoma lesions. Thus, erythema migrans and acrodermatitis chronica atrophicans have high levels of the T-cell-active chemokines CXCL9 and CXCL10, whereas borrelial lymphocytoma has high levels of the B-cell-active chemokine CXCL13.
Development of a monoclonal antibody specific to the endonuclease domain of the human LINE-1 ORF2 protein (2014)

LINE-1 (L1) retrotransposons are common occupants of mammalian genomes representing about a fifth of the genetic content. Ongoing L1 retrotransposition in the germ line and somatic tissues has contributed to structural genomic variations and disease-causing mutations in the human genome. L1 mobilization relies on the function of two, self-encoded proteins, ORF1 and ORF2. The ORF2 protein contains two characterized domains: endonuclease and reverse transcriptase. Using a bacterially purified endonuclease domain of the human L1 ORF2 protein, we have generated a monoclonal antibody specific to the human ORF2 protein. We determined that the epitope recognized by this monoclonal antibody includes amino acid 205, which is required for the function of the L1 ORF2 protein endonuclease. Using an in vitro L1 cleavage assay, we demonstrate that the monoclonal anti-ORF2 protein antibody partially inhibits L1 endonuclease activity without having any effect on the in vitro activity of the human AP endonuclease. Overall, our data demonstrate that this anti-ORF2 protein monoclonal antibody is a useful tool for human L1-related studies and that it provides a rationale for the development of antibody-based inhibitors of L1-induced damage.
Nano- and Microstructures for Thin-Film Evaporation—A Review (2014)

Evaporation from thin films is a key feature of many processes, including energy conversion, microelectronics cooling, boiling, perspiration, and self-assembly operations. The phase change occurring in these systems is governed by transport processes at the contact line where liquid, vapor, and solid meet. Evidence suggests that altering the surface chemistry and surface topography on the micro- and the nanoscales can be used to dramatically enhance vaporization. The 2013 International Workshop on Micro- and Nanostructures for Phase-Change Heat Transfer brought together a group of experts to review the current state-of-the-art and discuss future research needs. This article is focused on the thin-film evaporation panel discussion and outlines some of the key principles and conclusions reached by that panel and the workshop attendees.
Continuous Arabic Sign Language Recognition in User Dependent Mode (2010)

Arabic Sign Language recognition is an emerging field of research. Previous attempts at automatic vision-based recog-nition of Arabic Sign Language mainly focused on finger spelling and recognizing isolated gestures. In this paper we report the first continuous Arabic Sign Language by building on existing research in feature extraction and pattern recognition. The development of the presented work required collecting a continuous Arabic Sign Language database which we designed and recorded in cooperation with a sign language expert. We intend to make the collected database available for the research community. Our system which we based on spatio-temporal feature extraction and hidden Markov models has resulted in an average word recognition rate of 94%, keeping in the mind the use of a high perplex-ity vocabulary and unrestrictive grammar. We compare our proposed work against existing sign language techniques based on accumulated image difference and motion estimation. The experimental results section shows that the pro-posed work outperforms existing solutions in terms of recognition accuracy.
A multiple-electrode array for a cochlear implant (1976)

A satisfactory multiple-electrode array for a cochlear implant has been developed. This can be passed around the turns of the cochlea if it is first introduced into the scala tympani through an opening in the apical turn.
China’s Maxim – Leave No Access Point Unexploited: The Hidden Story of China Telecom’s BGP Hijacking (2018)

Surprisingly, the voluntary 2015 Xi-Obama agreement stopping military forces from hacking commercial enterprises for economic gain did appear to dramatically reduce Chinese theft efforts against western targets. China's technological development process, however, was still dependent on massive expropriation of foreign R&D. This necessitated new ways to get information while still technically adhering to the agreement. Since the agreement only covered military activities, Chinese corporate state champions could be tasked with taking up the slack. But even Chinese multinationals, such as Huawei or ZTE, were already being viewed with suspicion. Instead data suggests the government opted to leverage a seemingly innocuous playerone that is normally viewed as a passive service providerto target the foundational infrastructure of the internet to bypass the agreement, avoid detection, and provide the necessary access to information.
A murein hydrolase is the specific target of bulgecin in Escherichia coli. (1992)

A deletion in the structural gene for the soluble lytic transglycosylase, the predominant murein hydrolase in the soluble fraction of Escherichia coli, has been constructed. The mutant grows normally but exhibits increased sensitivity toward mecillinam, a beta-lactam specific for penicillin-binding protein 2. In the presence of furazlocillin or other beta-lactams with a specificity for penicillin-binding protein 3 which normally cause filamentation, bulges were formed prior to rapid bacteriolysis. Similar morphological alterations are known to develop in wild type E. coli cells when furazlocillin is combined with bulgecin, an antibiotic of unusual glucosaminyl structure. It turned out that bulgecin specifically inhibits the Sl-transglycosylase in a noncompetitive manner. Since bulgecin shows some structural analogy to the murein subunits we postulate that the soluble lytic transglycosylase, in addition to its active site, has a recognition site for specific murein structures. The possibility of an allosteric modulation of the activity of the enzyme by changes in the structure of the murein sacculus is discussed.
Corrigendum to “Chronic hyperalgesic priming in the rat involves a novel interaction between cAMP and PKCε second messenger pathways” [Pain 113 (2005) 185–190] (2005)

Departments of Oral and Maxillofacial Surgery, Medicine, and Anatomy, and Program in Neuroscience, University of California, San Francisco, CA 94143-0440, USA *Corresponding author. Address: NIH Pain Center, University of California, Room C-555, Campus Box 0440, San Francisco, CA 94143-0440, USA. Tel.: +1 415 476 5108; fax: +1 415 476 6305. E-mail address:[email protected]
Production of Fish Protein Hydrolysates with Bacterial Proteases; Yield and Nutritional Value (1991)

ABSTRACT Three bacterial proteases were selected for their activity on casein per unit cost. Conditions for inactivation were determined. Fish protein was hydrolyzed without water addition. Protein was solubilized faster with Pescalase 560 than with HT‐200 or Protease N. Increase of protease concentration was associated with a quadratic increase in soluble nitrogen. Yields of 13 to 15% in terms of whole fish were obtained at the pilot plant. Products contained 83 to 86% protein of which 70 to 80% was soluble. Reactive lysine was 70 to 85%. Nutritional evaluation with rats indicated hydrolysates had 10 to 15% lower nutritional value than casein. Results suggest fish protein hydrolysates can be used in the food industry for their solubility and nutritional value.
Elimination of plasmid pMG110 from Escherichia coli by novobiocin and other inhibitors of DNA gyrase (1984)

The ability of novobiocin to eliminate (cure) the wild-type plasmid pMG110 from Escherichia coli has been compared with that of other inhibitors of the gyrase B subunit and of the gyrase A subunit. Novobiocin eliminated pMG110 , producing over 99% plasmid loss at concentrations two- to eightfold below the MIC for bacterial growth. Structurally related compounds ( clorobiocin , coumermycin A1, isobutyryl novenamine , and decarbamyl novobiocin) varied in their ability to eliminate pMG110 . Higher concentrations of drugs were required to eliminate pMG110 from a gyrB( Cour ) strain, implicating DNA gyrase in the curing phenomenon. For these drugs, the ratio of the concentration effecting maximal plasmid elimination to the MIC varied from 0.16 to 1.1, indicating that curing cannot be explained simply by inhibition of a pool of DNA gyrase equally available for replication of the bacterial chromosome and the plasmid DNA molecule. Inhibitors of the gyrase A subunit, nalidixic acid and oxolinic acid, eliminated pMG110 only to variable low levels. The differences in the ability of the gyrase A and B subunit antagonists to eliminate plasmids are discussed.
Optimum design, performance evaluation, and inherent limitations of DPCM encoders (1978)

The technological feasibility of implementing high-speed analog-to-digital (A/D) converters intended to achieve very high signal-to-noise (S/N) performance as well as long-term stability operation depends critically upon the intrinsic component and circuit imperfections. In studying DPCM techniques for A/D conversion, these effects were included in the design and analysis of two predictive DPCM systems, the first having digital predictor feedback while the second used an analog predictor filter in the feedback loop. In the presence of noise due to quantization and threshold inaccuracies, new optimum linear predictor coefficients that are signal statistics independent were obtained. The noise due to circuit inaccuracies was found to have a pronounced effect on the S/N performance for both the digital and the analog predictor DPCM configurations. On the basis of these newly derived results there are strong indications that, in the presence of circuit imperfections, the S/N performance attainable by a simply oversampled A/D converter constitutes an upper bound on performance for any DPCM system constructed with components of similar accuracy.