gebhart/patrepeval huggingface dataset:
BAP1 complex promotes transcription by opposing PRC1-mediated H2A ubiquitylation (2019)

In Drosophila, a complex consisting of Calypso and ASX catalyzes H2A deubiquitination and has been reported to act as part of the Polycomb machinery in transcriptional silencing. The mammalian homologs of these proteins (BAP1 and ASXL1/2/3, respectively), are frequently mutated in various cancer types, yet their precise functions remain unclear. Using an integrative approach based on isogenic cell lines generated with CRISPR/Cas9, we uncover an unanticipated role for BAP1 in gene activation. This function requires the assembly of an enzymatically active BAP1-associated core complex (BAP1.com) containing one of the redundant ASXL proteins. We investigate the mechanism underlying BAP1.com-mediated transcriptional regulation and show that it does not participate in Polycomb-mediated silencing. Instead, our results establish that the function of BAP1.com is to safeguard transcriptionally active genes against silencing by the Polycomb Repressive Complex 1.
Smoking, heavy physical work and low back pain: A four-year prospective study (1999)

Data from a community-based four-year prospective study were used to test the hypothesis that heavy physical work is a stronger predictor of low back pain in smokers than in non-smokers. Of 708 working responders without low back pain during the entire year prior to 1990, 562 (79%) completed a questionnaire four years later in 1994. A job involving heavy lifting and much standing in 1990 was a strong predictor of low back pain in smokers four years later [odds ratio (OR) = 5.53, 95% confidence interval (CI) = 1.93-15.84, p < 0.01) after having adjusted for other job characteristics, demographic factors, emotional symptoms, physical exercise and musculoskeletal pain elsewhere. In non-smokers, having a job with heavy lifting and much standing was not associated with low back pain. One explanation may be that smoking leads to reduced perfusion and malnutrition of tissues in or around the spine and causes these tissues to respond inefficiently to mechanical stress.
World Wide Web Page Design: A Structured Approach (1997)

SUMMARY In conclusion, homepage construction based on structured programming concepts makes it simpler to build a functional homepage. It also assists in organization of the homepage and provides for easier maintenance and modification. Figure 3 contains an example of a simple home page which incorporates the principles outlined in this article. As more libraries create their own Web sites, use of structured organization can provide a framework for control over the many variables in this process.
Preparation of 1-octene by ethylene tetramerization with high selectivity (2006)

二新奇 PNP ligands 被 ~ 1H-NMR，元素的分析，和集体系列综合了并且描绘。在有 Cr (III ) 和辅催化剂毛的联合，他们产生活跃催化系统有高催化活性和高选择到的那 tetramerize 乙烯生产 1-octene。结果证明这些催化剂系统能催化乙烯 tetramerization，与直到 0.89 X 10 ~ 的高催化活性在产品的 C_8 的 6 g/mol Cr.h，和选择是 72.52% ，并且在 C_8 的 1 石蜡的百分比切 is97.87 百分比。
Dielectric properties of aluminium solid electrolytic capacitor using conduction polymer (2002)

Digitalization in electronic systems is required with capacitors which are large capacitance with small size, low impedance at high frequency, and high reliability. The fabrication and properties of aluminium solid electrolytic capacitors are investigated. Employing a conducting polymer film such as, polypyrrole as solid electrolyte, solid type aluminium electrolytic capacitors were made. The surface of insulating oxide is covered with conducting polymer layer prepared by chemical oxidative polymerization. Thereafter this conducting layer is covered with conducting polymer prepared by electrochemical polymerization. The dielectric properties of these capacitors were also measured and discussed. Regarding the frequency characteristics of the trial capacitor, impedance and ESR at high frequency is lower than those of the stacked type film capacitor. It is also confirmed that the temperature coefficient of capacitance and dissipation factor of the capacitor is lower than that of the film capacitor and liquid type aluminium electrolytic capacitor.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Canonical Androstenedione Reduction Is the Predominant Source of Signaling Androgens in Hormone-Refractory Prostate Cancer (2014)

Abstract Purpose: It has been recognized for almost a decade that concentrations of signaling androgens sufficient to activate the androgen receptor are present in castration-resistant prostate cancer tissue. The source of these androgens is highly controversial, with three competing models proposed. We, therefore, wished to determine the androgenic potential of human benign and malignant (hormone-naïve and treated) prostate tissue when incubated with various precursors and examine concomitant changes in enzyme expression. Experimental Design: Freshly harvested prostate tissue [benign, hormone-naïve, and hormone-refractory prostate cancer (HRPC)] was incubated in excess concentrations of cholesterol, progesterone, DHEA, androstenedione, or testosterone for 96 hours, and steroid concentrations in the conditioned media measured by gas chromatography–mass spectroscopy. Changes in the expression of androgen synthetic and/or degradative enzymes were determined by expression microarray and qPCR. Significant changes were confirmed in an independent dataset. Results: Of the precursor molecules tested, only incubation with androstenedione gave rise to significant concentrations of signaling androgens. Although this was observed in all tissue types, it occurred to a significantly greater degree in hormone-refractory compared with hormone-naïve cancer. Consistent with this, gene set enrichment analysis of the expression microarray data revealed significant upregulation of 17HSD17B activity, with overexpression of the canonical enzyme AKR1C3 confirmed by qPCR in the same samples and in a publicly available expression dataset. Importantly, we found no evidence to support a significant contribution from either the “backdoor” or “5-α dione” pathway. Conclusions: Reduction of androstenedione to testosterone by the canonical HSD17B AKR1C3 is the predominant source of signaling androgens in HRPC. Clin Cancer Res; 20(21); 5547–57. ©2014 AACR.
1,4,7,10-Tetraazacyclododecane Metal Complexes as Potent Promoters of Carboxyester Hydrolysis under Physiological Conditions (2007)

New 1,4,7,10-tetrazacyclododecane ([12]aneN4 or cyclen) ligands with different heterocyclic spacers (triazine and pyridine) of various lengths (bi- and tripyridine) or an azacrown pendant and their mono- and dinuclear Zn(II), Cu(II), and Ni(II) complexes have been synthesized and characterized. The pKa values of water molecules coordinated to the complexed metal ions were determined by potentiometric pH titrations and vary from 7.7 to 11.2, depending on the metal-ion and ligand properties. The X-ray structure of [Zn2L2]mu-OH(ClO4)3.CH3CN.H2O shows each Zn(II) ion in a tetrahedral geometry, binding to three N atoms of cyclen (the average distance of Zn-N = 2.1 A) and having a mu-OH bridge at the apical site linking the two metal ions (the average distance of Zn-O- = 1.9 A). The distance between the Zn(II) ion and the fourth N atom is 2.6 A. All Zn(II) complexes promote the hydrolysis of 4-nitrophenyl acetate (NA) under physiological conditions, while those of Cu(II) and Ni(II) do not have a significant effect on the hydrolysis reaction. The kinetic studies in buffered solutions (0.05 M Tris, HEPES, or CHES, I = 0.1 M, NaCl) at 25 degrees C in the pH range of 6-11 under pseudo-first-order reaction conditions (excess of the metal complex) were analyzed by applying the method of initial rates. Comparison of the second-order pH-independent rate constants (kNA, M-1 s-1) for the mononuclear complexes ZnL1, ZnL3, and ZnL8, which are 0.39, 0.27, and 0.38, respectively, indicates that the heterocyclic moiety improves the rate of hydrolysis up to 4 times over the parent Zn([12]aneN4) complex (kNA = 0.09 M-1 s-1). The reactive species is the Zn(II)-OH- complex, in which the Zn(II)-bound OH- acts as a nucleophile, which attacks intermolecularly the carbonyl group of the acetate ester. For dinuclear complexes Zn2L2, Zn2L4, Zn2L5, Zn2L6, and Zn2L7, the mechanism of the reaction is defined by the degree of cooperation between the metal centers, determined by the spacer length. For Zn2L7, having the longest triaryl spacer, the two metal centers act independently in the hydrolysis; therefore, the reaction rate is twice as high as the rate of the mononuclear analogue (kNA = 0.78 M-1 s-1). The complexes with a monoaryl spacer show saturation kinetics with the formation of a Michaelis-Menten adduct. Their hydrolysis rates are 40 times higher than that of the Zn[12]aneN4 system (kNA approximately 4 M-1 s-1). Zn2L6 is a hybrid between these two mechanisms; a clear saturation curve is not visible nor are the metal cores completely independent from one another. Some of the Zn(II) complexes show a higher hydrolytic activity under physiological conditions compared to other previously reported complexes of this type.
A Study on Surface Roughness During Fused Deposition Modelling: A Review (2018)

Rapid Prototyping (RP) is technology used to produce a physical model or prototype directly from three-dimensional Computer-Aided-Design (3D CAD) data in a very short time. Fused Deposition Modelling (FDM) is a process for developing RP objects from plastic material by laying tracks of semi molten plastic filament on to a platform in precise layers from bottom to top. RP has been extensively used by manufacturers from different backgrounds to accelerate their product development and cycle time without neglecting product quality. In the RP process, surface finish is an important criterion as it can influence the part precision, post-handling expenses and functionality of the part. This paper presents a review of current studies on surface roughness using FDM. This paper also highlights design of experiments (DOE) and its association with surface finish.
User requirements for designing complex systems on silicon (2002)

In this paper we define the requirements for a specification environment, called POPE, suited to describe complex user-end telecommunication systems and useful as a backbone for automation of the implementation path towards integration of such systems on silicon. The definition of these requirements has been driven by the design and implementation of a mobile terminal for spread spectrum satellite communications which is considered, in the context of our work, to be representative for the definition of a system.
Wireless battery management system (2013)

In this paper, we present an innovative wireless battery management system (WBMS™)that addresses the issues of the conventional BMS architecture. The WBMS™ adopts wireless communication technologies based on a proprietary Wireless Battery Area Network (WiBaAN™) protocol [1]. The WBMS™ consists of a multitude of energy-autonomous micro-sensors mounted on each battery cell, and a master module that collects and processes the data from the micro-sensors. The WiBaAN™ protocol defines the syntax, semantics and synchronization of the messages between the sensors and the master module. The WBMS™ will enable a battery pack that is simple, light, reliable, and most importantly, inexpensive. The new wireless architecture can completely eliminate the sensing wire-harness and connectors. The WBMS™ is designed in such a way that a single master module can communicate with up to a few thousands of sensors. Its star topology makes it possible to eliminate the intermediate slave modules between the master module and the sensors. No isolators are needed due to the wireless nature of the communication. Reduced number of components and connections means less failure points, especially in a harshly vibratory environment.
Dissociation of the receptor for immunoglobulin E in mild detergents (1985)

We previously showed that, in the absence of phospholipids, exposure of the tetrameric receptor for immunoglobulin E to mild detergents dissociates the intact beta chain and two gamma chains from the alpha chains. Having developed a practical method for assaying the dissociation, we have now explored a variety of different detergents, detergent concentrations, temperatures, times, salts, pHs, and other factors that influence the detergent-induced dissociation. Our findings should be useful for optimizing the stability of the receptor and for future studies on recombination of the subunits. The data suggest the following: (1) The critical perturbant is micellar detergent. (2) Unlike solubilization of membranes, where a molar ratio of micellar detergent:lipid of 2 is adequate, dissociation of the receptor is incomplete even at molar ratios of micellar detergent:receptor of greater than 10(5) and may be limited by a reversible component. (3) Detergents that are best for solubilizing membranes are also best for dissociating the receptors. (4) The latter observation and other data implicate bound lipid as stabilizing the receptor. Our findings may be applicable to the study of interactions between membrane proteins in general.
Diurnal Variation of Hepatic Antioxidant Gene Expression in Mice (2012)

Circadian variations and gender differences in transcript levels of antioxidant genes exist in mouse liver, which could affect body responses to oxidative stress at different times of the day.
Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay (2013)

The use of Gaussia luciferase in a multiplex assay can have several advantages over the singleplex method for an experimental setup. Issues such as intersample variability, screening purposes, efficiency, and in vivo applications can be addressed using a multiplex assay. Here we describe a functional reporter multiplex method using Gaussia luciferase fused to epitope tags to identify the different reporters that are expressed. Tag specific antibodies are used to bind and separate the tagged luciferase reporters.
Serum Somatomedin Levels in Adults with Chronic Renal Failure: TheImportance of Measuring Insulin-Like Growth Factor I (IGF-I) and IGF-II in Acid-Chromatographed Uremic Serum∗ (1986)

Somatomedin levels measured by radioreceptor assay (RRA) or RIA on acid-ethanol-extracted or unextracted serum from patients with uremia are quite variable relative to normal values. We have investigated whether compounds accumulating in uremic serum were interfering with these RRA and RIA measurements. Insulin-like growth factor I (IGF-I) and IGF-II were separated from carrier proteins by either Sephadex G-50 acid chromatography or acid-ethanol extraction. IGF-I was measured by RIA, and IGF-II was determined by rat placental membrane RRA. IGF-I levels in the acid-ethanol extracts of serum from eight uremic adults were only 50% of the levels found in seven normal subjects, and IGF-II levels in these uremic patients were 350% of normal values. However, these significant differences were not found when comparable serum samples were acid chromatographed rather than acid-ethanol extracted; instead, IGF-I levels were 343 +/- 168 (mean +/- SD) ng/ml in uremic patients and 325 +/- 54 ng/ml in normal subjects, while IGF-II levels were 780 +/- 215 ng/ml in uremic patients and 588 +/- 46 ng/ml in normal subjects. To determine whether compounds that interfere with somatomedin assays were present in the acid-ethanol extracts of uremic serum, we acid chromatographed these extracts and found that a compound eluting in the carrier protein fraction of the uremic extract artifactually decreased IGF-I levels measured by RIA and increased IGF-II levels measured by RRA. We also found that unsaturated carrier protein binding of IGF-I and IGF-II was significantly increased in the carrier protein fraction of uremic acid-ethanol extracts at the dilutions used for somatomedin assays. We conclude that acid chromatography of unextracted or acid-ethanol-extracted uremic serum is necessary to remove a compound, probably unsaturated carrier protein, which interferes with RIA of IGF-I and RRA of IGF-II. Once this compound is removed, serum IGF-I levels (RIA) in uremic patients are not different from normal, and serum IGF-II levels (RRA) are not lower than normal.
Proteomic analysis of vascular endothelial cells in response to laminar shear stress (2007)

Isotope-coded affinity tags (cICAT) coupled with mass spectrometric analysis is one of the leading technologies for quantitative proteomic profiling and protein quantification. We performed proteomic analysis of bovine aortic endothelial cells (BAEC) in response to laminar shear stress using cICAT labeling coupled with LC-MS/MS. Protein expressions in BAEC under 15 dynes/cm2 of shear stress for 10 min, 3 h, and 6 h were compared with matched stationary controls. Analysis of each sample produced 1800-2400 proteins at >or=75% confidence level. We found 142, 213, and 186 candidate proteins that were up- or down-regulated by at least two-fold after 10 min, 3 h, and 6 h of shear stress, respectively. Some of these proteins have known cellular functions and they encompass many signaling pathways. The signaling pathways that respond to shear stress include those of integrins, G-protein-coupled receptors, glutamate receptors, PI3K/AKT, apoptosis, Notch and cAMP-mediated signaling pathways. The validity of the mass spectrometric analysis was also confirmed by Western blot and confocal immunofluorescence microscopy. The present quantitative proteomic analysis suggests novel potential regulatory mechanisms in vascular endothelial cells in response to shear stress. These results provide preliminary footprints for further studies on the signaling mechanisms induced by shear stress.
Bit allocation algorithm for multi-user adaptive OFDM system with adaptive trellis coded MQAM (2003)

In this paper, a multiuser adaptive OFDM (orthogonal frequency-division multiplexing) transmission system is simulated. A bit allocation algorithm is considered in this system We propose a DCA-DBA-DCA algorithm using the adaptive trellis coded modulation (ATCM) and compare its performance with fixed OFDM and other adaptive algorithms. The simulation shows that DCA can increase system performance by 5/spl sim/6 dB and our system can greatly reduce the computational cost while maintain a fairly good QoS. By changing the interval between two DCA (dynamic channel allocations) our system can be used in various environments.
Quasi-bandlimited properties of Radon transforms and their implications for increasing angular sampling densities (1998)

The n-dimensional (n-D) radon transform, which forms the mathematical basis for a broad variety of tomographic imaging applications, can be viewed as an n-D function in n-D sinogram space. Accurate reconstruction of continuous or discrete tomographic images requires full knowledge of the radon transform in the corresponding n-D sinogram space. In practice, however, one can have only a finite set of discrete samples of the radon transform in the sinogram space. One often derives the desired full knowledge of the radon transform from its discrete samples by invoking various interpolation algorithms. According to the Wittaker-Shannon sampling theorem, a necessary condition for a full and unique recovery of the radon transform from its discrete samples is that the radon transform itself be bandlimited. Therefore, it is necessary to analyze the bandlimited properties of the radon transform. In this work, we analyze explicitly the bandlimited properties of the radon transform and show that the radon transform is mathematically quasi-bandlimited [or essentially bandlimited] in two quantitative senses and can essentially be treated as bandlimited in practice. The quasi-bandlimited properties can be used for increasing the angular sampling density of the radon transform.
ChemInform Abstract: HIGH‐PRESSURE SYNTHESES OF CUBIC BORON NITRIDE USING WATER, UREA, AND BORIC ACID CATALYSTS (1979)

Abstract Bei Verwendung von H 2 O, Harnstoff oder Borsäure als Katalysatoren geht hexagonales BN bei hohen Drücken und Temp. in kubisches BN über.
The Reactions of the Dimers of Hexafluoropropene with <i>O</i>-Nucleophiles (1976)

Abstract The reactions of the dimers of hexafluoropropene with alcohols and phenol in the presence of triethylamine, and with sodium alkoxides and phenolate were carried out. Of the two dimers, perfluoro-2-methyl-2-pentene (Dimer 2) was much more reactive than perfluoro-4-methyl-2-pentene (Dimer 1). While the dimer 2 and O-nucleophiles gave various addition and substitution products very easily, the dimer 1 reacted only with sodium alkoxides to give trialkoxylated products.
Dielectric and electromechanical properties of barium titanate single crystals grown by templated grain growth (2000)

Single crystals of BaTiO(3) were grown by templated grain growth (TGG). TGG involves contacting a single crystal "template" to a sintered polycrystalline matrix, then heating the assemblage to a temperature that promotes the migration of the single crystal boundary through the matrix. In this investigation the properties of millimeter-sized, plate-shaped and bar-shaped, single crystals of BaTiO(3) grown by TGG were examined in order to compare the results to single crystals grown by conventional methods. A dielectric constant, epsilon(33)(T)/epsilon(0), of 260, polarizations of P(R)~16 microC/cm(2) and P(sat)~21.5 microC/cm(2), electromechanical coupling coefficients of k(33)~0.51 and k(31)~0.18, and a piezoelectric coefficient of d(33)~140 pC/N were achieved. Entrapped porosity in the crystals made fully poling the crystals difficult.
Multiline, superbroadband and sun-color oscillation of a LiF:F_2^- color-center laser (1997)

We report experimental and theoretical studies of the temporal, spectral, and spatial features of a superbroadband laser. The results obtained show that the superbroadband room-temperature operable LiF:F(2)(-) color-center laser can provide low-coherence, high-intensity laser radiation with a spectral width of 1400 A centered at 1.14 mum and 700 A in the visible range from green to red and exhibit good spatial collimation with a divergence of between 5 and 6 mrad. Oscillation of all the lines of a superbroadband spectrum is completely synchronous and occurs almost simultaneously with the pump pulse, exhibiting 4-9-ns pulse delay at 20-ns pump pulse duration. Second-harmonic generation of superbroadband oscillation spectrum was realized with an overall efficiency of 10%.
Studies on pyrrolidinones. Synthesis of 5‐(5‐oxo‐2‐pyrrolidinyl)‐1,2,4‐triazole‐3‐thione derivatives (1989)

Abstract Starting from readily available pyroglutamic esters 1 , some new 1,2,4‐triazole‐3‐thione derivatives, bonded to a pyrrolidinone ring were synthesized and are characterized by their spectral data.
Compressing 3-D Human Motions via Keyframe-Based Geometry Videos (2014)

This paper presents keyframe-based geometry video (KGV), a novel framework for compressing 3-D human motion data by using geometry videos. Given a motion data encoded in a geometry video (GV) format, our method extracts the keyframes and produces a reconstruction matrix. Then it applies the video compression technique (e.g., H.264/Advanced Video Coding) to the reordered keyframes, which can significantly reduce the spatial and temporal redundancy in the KGV. We develop a rate distortion-based optimization algorithm to determine the parameters (i.e., the number of keyframes and quantization parameter) leading to optimal performance. Experimental results show that the proposed KGV framework significantly outperforms the existing GV techniques in terms of both the rate distortion performance and visual quality. Besides, the computational cost of the KGV is rather low at the decoder, making it highly desirable for power-constrained devices. Last but not least, our method can be easily extended to progressive compression with heterogeneous communication network.