gebhart/patrepeval huggingface dataset:
Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast (2013)

We investigate the immobilization of a model system of functionalized yeast that surface-display enhanced green fluorescent protein (eGFP) within chemically crosslinked polyvinyl alcohol (PVA) nanofibers. Yeast is incorporated into water insoluble nanofibrous materials by direct electrospinning with PVA followed by vapor phase chemical crosslinking of the polymer. Incorporation of yeast into the fibers is confirmed by elemental analysis and the viability is indicated by live/dead staining. Following electrospinning and crosslinking, we confirm that the yeast maintains its viability as well as the ability to express eGFP in the correct conformation. This method of processing functionalized yeast may thus be a powerful tool in the direct immobilization of properly folded, active enzymes within electrospun nanofibers with potential applications in biocatalysis.
Developing ZigBee Deployment Guideline Under WiFi Interference for Smart Grid Applications (2010)

Smart grid is an intelligent power generation, distribution, and control system. ZigBee, as a wireless mesh networking scheme low in cost, power, data rate, and complexity, is ideal for smart grid applications, e.g., real-time system monitoring, load control, and building automation. Unfortunately, almost all ZigBee channels overlap with wireless local area network (WLAN) channels, resulting in severe performance degradation due to interference. In this paper, we aim to develop practical ZigBee deployment guideline under the interference of WLAN. We identify the “Safe Distance” and “Safe Offset Frequency” using a comprehensive approach including theoretical analysis, software simulation, and empirical measurement. In addition, we propose a frequency agility-based interference avoidance algorithm. The proposed algorithm can detect interference and adaptively switch nodes to “safe” channel to dynamically avoid WLAN interference with small latency and small energy consumption. Our proposed scheme is implemented with a Meshnetics ZigBit Development Kit and its performance is empirically evaluated in terms of the packet error rate (PER) using a ZigBee and Wi-Fi coexistence test bed. It is shown that the empirical results agree with our analytical results. The measurements demonstrate that our design guideline can efficiently mitigate the effect of WiFi interference and enhance the performance of ZigBee networks.
A study of error sources in current steering digital-to-analog converters (2004)

This work discusses a number of error sources which potentially limit the distortion and SNR performance of modern current steering digital-to-analog converter designs. Static matching errors, as well as timing and non-linear output impedance errors, are presented along with possible design solutions.
Design and Synthesis of [<sup>111</sup>In]DTPA−Folate for Use as a Tumor-Targeted Radiopharmaceutical (1997)

Folate-conjugated metal chelates have been proposed as potential imaging agents for cancers that overexpress folate receptors. In a previous study, folic acid was linked through its gamma-carboxyl group to deferoxamine (DF), and the 67Ga-labeled complex ([67Ga]DF-folate) was examined for in vivo tumor targeting efficiency in athymic mice with a human tumor cell implant. Although superb tumor-to-background contrast was obtained, slow hepatobiliary clearance would compromise imaging of abdominal tumors such as ovarian cancer. In the present study, folic acid was conjugated to an alternative chelator, diethylenetriaminepentaacetic acid (DTPA), via an ethylenediamine spacer. The desired DTPA-folate (gamma) regioisomer was synthesized by two different approaches, purified by reversed phase column chromatography, and characterized mainly by analytical HPLC, mass spectroscopy, and NMR. In cultured tumor cells, uptake of [111In]DTPA-folate (gamma) was found to be specific for folate receptor-bearing cells, and the kinetics of uptake were similar to those of free folate and other folate-conjugated molecules. In the normal rat, intravenously administered [111In]DTPA-folate (gamma) was found to be rapidly excreted into the urine, giving intestinal levels of radiotracer 10-fold lower than those observed with [67Ga]DF-folate (gamma) at 4 h. In a preliminary mouse imaging study, a folate receptor-positive KB cell tumor was readily visualized by gamma scintigraphy 1 h following intravenous administration of [111In]DTPA-folate (gamma).
Permanent Magnet AC Disc Motor Electric Vehicle Drive (1983)

The principal objective of this work was to establish the feasibility of the axial flux permanent magnet ac disc motor with load-commutated inverter as a candidate for an electric passenger vehicle drive system component. Three motors were built in the course of the project. The first, known as the proof-of-principal (POP) motor, was built to demonstrate the electromagnetic concepts and establish design data. It was built with rare earth magnets and a dc motor type random winding. The second motor, known as the Functional Model A (FM(A)) motor, was built for full performance and high speed (11,000 rpm). Alnico 8E magnets were used to stimulate manganese-aluminum. A three-layer air gap winding was used in the stator. This motor did not perform as predicted due primarily to demagnetization of the magnets and, secondarily, to imbalance in the windings. The third motor, known as Functional Model B (FM(B)), corrected these problems by use of rare earth magnets and a novel, single-layer chain winding. The motor performed well and in excess of contract requirements electromagnetically, thermally, and mechanically. A peak efficiency of 93% was measured at cycling power when the motor was being driven by the inverter. Several modifications were proposed to raise the efficiency to 95%.
Syntagmatic Kernels: a Word Sense Disambiguation Case Study (2006)

In this paper we present a family of kernel functions, named Syntagmatic Kernels, which can be used to model syntagmatic relations. Syntagmatic relations hold among words that are typically collocated in a sequential order, and thus they can be acquired by analyzing word sequences. In particular, Syntagmatic Kernels are defined by applying a Word Sequence Kernel to the local contexts of the words to be analyzed. In addition, this approach allows us to define a semi supervised learning schema where external lexical knowledge is plugged into the supervised learning process. Lexical knowledge is acquired from both unlabeled data and hand-made lexical resources, such as WordNet. We evaluated the syntagmatic kernel on two standard Word Sense Disambiguation tasks (i.e. English and Italian lexical-sample tasks of Senseval-3), where the syntagmatic information plays a crucial role. We compared the Syntagmatic Kernel with the standard approach, showing promising improvements in performance. 1
Bisbenzimidazoles. Potent inhibitors of rhinoviruses (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBisbenzimidazoles. Potent inhibitors of rhinovirusesWilliam R. Roderick, Carl W. Nordeen Jr., Anne M. Von Esch, and Raynor N. AppellCite this: J. Med. Chem. 1972, 15, 6, 655–658Publication Date (Print):June 1, 1972Publication History Published online1 May 2002Published inissue 1 June 1972https://pubs.acs.org/doi/10.1021/jm00276a023https://doi.org/10.1021/jm00276a023research-articleACS PublicationsRequest reuse permissionsArticle Views305Altmetric-Citations44LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An innovative RTP-based residential power scheduling scheme for smart grids (2011)

This paper proposes a Real-Time Pricing (RTP)-based power scheduling scheme as demand response for residential power usage. In this scheme, the Energy Management Controller (EMC) in each home and the service provider form a Stackelberg game, in which the EMC who schedules appliances' operation plays the follower level game, and the provider who sets the real-time prices according to current power usage profile plays the leader level game. The sequential equilibrium is obtained through the information exchange between them. Simulation results indicate that our scheme can not only save money for consumers, but also reduce peak load and the variance between demand and supply, while avoiding the "rebound" peak problem.
Crystallization kinetics and polymorphic transformations in polybutene-1 (1965)

When subcooled from the melt to any temperature between about 90 to 110 °C, polybutene-1 transforms at a readily measurable rate to a crystalline phase denoted "form 2," which is typically spherulitic. The kinetics of this process have been measured by optical and dilatometric techniques. The results are consistent with the concepts that the initiation is heterogeneous, that the initiation is followed by spherical growth (Avrami <i>n</i>=3), and that the growth rate is nucleation controlled. Values arc obtained for the surface free energies and related quantities that are consistent with crystallization by chain folding: <i>σ</i>=7.2 erg/cm<sup>2</sup> or 7.2 mJ/m<sup>2</sup>, <i>σ</i> <sub><i>e</i></sub> =15.5 erg/cm<sup>2</sup> or 15.5 mJ/m<sup>2</sup>, and work of chain folding <i>q</i>=1.7 × 10<sup>-13</sup> erg/fold. (For comparison, <i>q</i> is 2.1 × 10<sup>-13</sup> erg/fold for polyethylene.) The work of chain folding of polybutene-1 is also compared with that of polychlorotrifluoroethylene, and it is shown that an increase of <i>q</i> is connected with an increase of equilibrium melting temperature. Crystal form 2 subsequently converts slowly near room temperature to a crystalline phase denoted "form 1" of different helicity and density, and the rate of this crystal-crystal transformation is also studied. The Avrami parameter for the form 2→form 1 process is <i>n</i> = 2. The process appears to be nucleation controlled. It is shown that form 1 is, everywhere below its melting point, the stable form. The equilibrium melting temperature for form 2 is estimated to be about 128 °C, and for form 1 about 141 °C. A degree of crystallinity scale based on specific volume is established. The degree of crystallinity of form 2 is about 52 percent, and after conversion to form 1, the crystallinity is about 77 percent.
Ubiquitin-proteasome-dependent degradation of mammalian ER stearoyl-CoA desaturase (2006)

Mammalian Δ9 stearoyl-CoA desaturase 1 (SCD1) is a key enzyme in the biosynthesis of mono-unsaturated fatty acids in the endoplasmic reticulum (ER). It is a short-lived multispanning ER membrane protein, reported to be degraded by the ubiquitin-proteasome-independent pathway. We have examined SCD1 protein degradation using cultured mammalian cells. Exogenously expressed SCD1 in CHO-K1 cells was localized to the ER and turned over with a half-life of ∼3 hours. Unexpectedly, proteasome inhibitors increased the half-life of SCD1 to ∼6 hours. Endogenously expressed SCD1 in adipocyte-differentiated NIH 3T3-L1 cells was also rapidly degraded in a proteasome inhibitor-sensitive manner. In the presence of proteasome inhibitors, polyubiquitylated SCD1 accumulated in the ER and interacted with AAA-ATPase p97, which is involved in ER-associated degradation (ERAD). The 66-residue N-terminal segment carrying the PEST sequence is mainly responsible for SCD1 degradation and this segment induced instability in an otherwise stable ER membrane protein. Furthermore, SCD1 was degraded constitutively irrespective of the cellular levels of unsaturated fatty acids, which strictly regulate SCD1 gene expression. These findings indicate that the ubiquitin-proteasome-dependent ERAD system is also involved in constitutive SCD1 degradation.
Measurement of back-scattered radiation from micro multileaf collimator into the beam monitor chamber from a dual energy linear accelerator (2007)

Measurements designed to find the collimator backscatter into the beam monitor chamber from Micro Multileaf collimator of 6 MV photon beams of the Siemens Primus linear accelerator were made with the help of dose rate feedback control. The photons and electrons backscattered from the upper and lower secondary collimator jaws give rise to a significant increase in the ion charge measured by monitor chamber. This increase varies between the different accelerators. The output measurements were carried out in air at the isocenter. The effect of collimator backscatter was investigated by measuring the pulse width, number of beam pulses per monitor unit, monitor unit rate and dose for different mMLC openings. These measurements were made with and without dose rate feedback control, i.e., with constant electron beam current in the accelerator. Monitor unit rate (MU/min) was almost constant for all field sizes. The maximum variation between the open and the closed feedback control circuits was 2.5%. There was no difference in pulse width and negligible difference in pulse frequency. Maximum value of backscattered radiation from the micro Multileaf collimator into the beam monitor chamber was found to be 0.5%.
Room-temperature excitons in wide-gap layered-oxysulfide semiconductor: LaCuOS (2001)

Sharp optical absorption and emission peaks near the band gap (Eg≈3.1 eV) were observed in LaCuOS polycrystalline thin films at room temperature. The absorption peak was able to be detected at temperatures as high as 490 K, and its intensity remarkably increased with decreasing temperature. The spectral position of the absorption peak and its temperature dependence almost agreed with those of the emission peak. It was concluded that the sharp absorption and emission peaks originate from excitons. On the basis of semiquantitative consideration about the excitons, it is suggested that the electronic-structure characteristic of the layered-crystal structure of LaCuOS is responsible for the stability of the excitons. The observation of the exciton absorption and emission at room temperature revealed that LaCuOS is a promising material for optoelectronic applications such as light-emitting devices.
A distributed adaptive control system for a quadruped mobile robot (2002)

A method by which reinforcement learning can be combined into a behavior based control system is presented. Behaviors which are impossible or impractical to embed as predetermined responses are learned through self-exploration and self-organization using a temporal difference reinforcement learning technique. This results in what is referred to as a distributed adaptive control system (DACS), which is, in effect, the robot's artificial nervous system. A DACS is developed for a simulated quadruped mobile robot and the locomotion behavior level is isolated and evaluated. At the locomotion level the proper actuator sequences are learned for all possible gaits and eventually graceful gait transitions are also learned. When confronted with an actuator malfunction, all gaits and transitions are adapted resulting in new limping gaits for the quadruped.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Eye-contact visual communication with virtual view synthesis (2005)

In this paper, we propose a new visual communication system where eye contact is possible by using a virtual image. The virtual image is obtained by view synthesis with stereo matching from two real camera views. We developed a region based dynamic programming (DP) approach with improved matching cost, occlusion cost and vertical smoothness constraint. We also proposed a fast view interpolation method. To achieve real time performance, we developed a hardware system. Furthermore, to avoid the reordering problem in the foreground region, a view change approach with disorder detection is adopted. Experimental results demonstrate the validity of our improved DP matching algorithm and eye-contact visual communication system.
The Attitudes of Professionals Towards Job Sharing (1987)

This paper investigates the attitudes of a sample of engineers, medical technologists and primary school teachers regarding the perceived effects that job sharing may have upon: (a) the working conditions of these professionals; (b) the supply and demand of labour to these professions; (c) the administration of these professionals; and (d) the social relationships and interactions of these professionals. The findings strongly support the introduction of job sharing into these professions as an overall benefit-cost ratio of 6:1 was obtained. The results of this study are then compared against a number of other recent Australian studies in this area.
Control of hypocotyl phototropism by phytochrome in a dicotyledonous seedling (<i>Sesamum indicum</i> L.) (1988)

Abstract The phototropic response in stems of higher plants is brought about by blue/UV light. The problem studied here is to what extent long‐wavelength light, which is absorbed by phytochrome, affects the phototropic response. A refined measurement of phototropism — a curvature index — was applied to the hypocotyl of the sesame seedling ( Sesamum indicum L.). The time course of the phototropic response was followed in continuous unilateral weak blue light (B, 460 nm, 8 mW m −2 ). Long term red light (R) pretreatments, operating through phytochrome, strongly increase the rate and extent of the phototropic response once it is elicited by unilateral B, while the pretreatments decrease the sensitivity towards B. If a R pulse is given immediately prior to the onset of unilateral B, the rate of the response is strongly reduced compared to the time course of curvature observed when the pretreatment was terminated with a long wavelength far‐red light (FR) pulse. R and FR were then applied simultaneously with unilateral B to manipulate the status of the phytochrome system during actual curvature. It was found that a low Pfr/P ratio (established by FR) stimulates the phototropic response far above the control (B alone), while a high Pfr/P ratio (established by R) reduces the response below the control. During bending a positive effect of phytochrome on the rate and extent of the phototropic response, which is saturated at a low level of Pfr, appears to be counteracted by an inhibitory effect which dominates at higher levels of Pfr, such as established by omnilateral R. However, if R is applied unilaterally from the same direction as B, R increases the rate of curvature. Apparently the sesame seedling is capable of detecting the direction of R relative to the direction of B. While a mechanistic explanation of these effects cannot be advanced at present, it is clear that the seedling is capable of super‐imposing information about the actual light conditions during bending on a ‘memory’ of the light conditions prior to the onset of bending. Thus, the previous as well as the actual light conditions determine its phototropic responsiveness.
Modeling the triggering of streamers in air by ultrashort laser pulses (2000)

The physical processes involved in the triggering of ionization waves (streamers) by ultrashort laser pulses, focused in air at 350 Torr and in a uniform electric field, are investigated by means of a one-dimensional (1-D) numerical model. The model describes the interaction of the laser pulse with air and takes into account many of the reactions in the laser-created plasma as well as the radial expansion of the plasma. Consequences of the model are that the threshold electric field for the appearance of streamers is an increasing function of the delay between the laser pulse and the electric field pulse and a decreasing function of the laser energy. Also, it appears that the electron temperature, the plasma density and radius, and the conduction of heat across the plasma boundaries play major roles in the capacity of the laser-created plasma to trigger streamers. The results of the model are compared with the available experimental data.
Overexpression of human aspartyl (asparaginyl) beta-hydroxylase is associated with malignant transformation. (2000)

The human aspartyl (asparaginyl) beta-hydroxylase (HAAH) is a highly conserved enzyme that hydroxylates epidermal growth factor-like domains in transformation-associated proteins. We previously reported overexpression of the HAAH gene in human hepatocellular carcinomas and cholangiocarcinomas (L. Lavaissiere et al., J. Clin. Investig., 98: 1313-1323, 1996). In the present study, we determined whether HAAH protein overexpression was linked to cellular proliferation or malignant transformation of bile ducts by using a human disease and rat model of bile duct proliferation. In addition, the transforming properties of the AAH genes were assessed by transient and stable transfection of NIH-3T3 cells with human and murine wild-type as well as mutant cDNA constructs that lacked hydroxylation activity. Cellular characteristics of the malignant phenotype were assessed by formation of transformed foci, growth in soft agar, and tumor development in nude mice. We found that HAAH gene expression was undetectable during bile duct proliferation in both human disease and rat models as compared with cholangiocarcinoma. Overexpression of HAAH in NIH-3T3 cells was associated with generation of a malignant phenotype, and enzymatic activity was required for cellular transformation. These findings suggest that overexpression of HAAH is linked to cellular transformation of biliary epithelial cells.
String Instability in Classes of Linear Time Invariant Formation Control With Limited Communication Range (2010)

This paper gives sufficient conditions for string instability in an array of linear time-invariant autonomous vehicles with communication constraints. The vehicles are controlled autonomously and are subject to a rigid or semi-rigid formation policy. The individual controllers are assumed to have a limited range of forward and backward communication with other vehicles. Sufficient conditions are given that imply a lower bound on the peak of the frequency response magnitude of the transfer function mapping a disturbance to the leading vehicle to a vehicle in the chain. This lower bound quantifies the effect of spacing separation policy, intervehicle communication policy, and vehicle settling response performance. These results extend earlier works to give a unified treatment of heterogeneous, non-nearest neighbor communication and semi-rigid one-dimensional formation control.