gebhart/patrepeval huggingface dataset:
Experimental Research of Fufangsangen Decoction on Pulmonary Carcinoma Transfer of T739 Mice (2005)

Objective: To study the mechanism of Fufangsangen (FFSG) decoction on prohibiting LA795 pulmonary carcinoma transfer and provide experimental evidence for preventing and treating pulmonary carcinoma transfer with traditional Chinese medicine. Methods: T739 mice with high transferred LA795 pulmonary carcinoma were experimentally treated in whole by feeding with FFSG. The carcinoma prohibition ratio and the anti-lung transfer ratio of T739 mice were observed and compared with those treated by CTX. Results: The carcinoma prohibition ratios in both groups were 27.2% and 89.4% respectively, the anti-lung transfer ratios 45.7% and 59.0% respectively, their being significant difference in statistics. Conclusion: FFSG has some prohibition effect on the growth of T739 transferred tumor and anti-lung transfer effect.
Identification of a tight junction–associated guanine nucleotide exchange factor that activates Rho and regulates paracellular permeability (2003)

Rho family GTPases are important regulators of epithelial tight junctions (TJs); however, little is known about how the GTPases themselves are controlled during TJ assembly and function. We have identified and cloned a canine guanine nucleotide exchange factor (GEF) of the Dbl family of proto-oncogenes that activates Rho and associates with TJs. Based on sequence similarity searches and immunological and functional data, this protein is the canine homologue of human GEF-H1 and mouse Lfc, two previously identified Rho-specific exchange factors known to associate with microtubules in nonpolarized cells. In agreement with these observations, immunofluorescence of proliferating MDCK cells revealed that the endogenous canine GEF-H1/Lfc associates with mitotic spindles. Functional analysis based on overexpression and RNA interference in polarized MDCK cells revealed that this exchange factor for Rho regulates paracellular permeability of small hydrophilic tracers. Although overexpression resulted in increased size-selective paracellular permeability, such cell lines exhibited a normal overall morphology and formed fully assembled TJs as determined by measuring transepithelial resistance and by immunofluorescence and freeze-fracture analysis. These data indicate that GEF-H1/Lfc is a component of TJs and functions in the regulation of epithelial permeability.
Advances in Aptamer-Based Biosensors for Food Safety (2011)

The presence of unsafe levels of chemical compounds, toxins, and pathogens in food constitutes a growing public health problem that necessitates new technology for the detection of these contaminants along the food continuum from production to consumption. While traditional techniques that are highly selective and sensitive exist, there is still a need for simpler, more rapid and cost-effective approaches to food safety testing. Within this context, the field of food safety biosensors has emerged. Biosensors consist of a specific molecular recognition probe targeting an analyte of interest and a means of converting that recognition event into a measurable signal. As molecular recognition is the foundation of biosensing, there has been increased focus on the development of new molecular recognition probes for food-safety related molecular targets. Antibodies have been the gold standard in molecular recognition for several decades and have been incorporated widely into biosensors and assays relating to food (Ricci et al., 2007). Despite their applicability to food monitoring, they are not without their disadvantages, which are primarily linked to the requirement that antibody generation is an in vivo process. For example, highly toxic substances are not conducive to antibody generation. Furthermore, the batch to batch reproducibility of antibody generation can be less than satisfactory. Compounding these limitations is the fact that antibodies have short shelf-lives and can be challenging to chemically modify for incorporation into a biosensor platform. Nevertheless, the affinity and specificity of antibodies for their molecular targets make them convenient receptors for biosensing strategies. Many of the disadvantages described above could be avoided with a molecular recognition probe of synthetic origin that would still maintain the required specificity and affinity. Because of their in vitro selection and production, the relatively new technology of aptamers has emerged as a viable alternative for use in biosensor platforms (Mascini, 2009). This chapter will focus on the recent literature in aptamers for food safety related targets, as well as the biosensor platforms in which these probes have been incorporated.
Metabolism of Hydroxy Fatty Acids (1965)

Journal Article Metabolism of Hydroxy Fatty Acids: IV. Influence of cis- and trans-Double Bond in Hydroxy Fatty Acid Metabolism by Escherichia coli K 12 Get access MICHINAO MIZUGAKI, MICHINAO MIZUGAKI Institute of Pharmacy, Tohoku University School of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar MITSURU UCHIYAMA, MITSURU UCHIYAMA Institute of Pharmacy, Tohoku University School of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar SEIICHI OKUI SEIICHI OKUI Institute of Pharmacy, Tohoku University School of MedicineSendai Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 58, Issue 2, August 1965, Pages 174–178, https://doi.org/10.1093/oxfordjournals.jbchem.a128180 Published: 01 August 1965 Article history Received: 06 May 1965 Published: 01 August 1965
Basal ganglia activity patterns in parkinsonism and computational modeling of their downstream effects (2012)

The availability of suitable animal models and the opportunity to record electrophysiologic data in movement disorder patients undergoing neurosurgical procedures has allowed researchers to investigate parkinsonism-related changes in neuronal firing patterns in the basal ganglia and associated areas of the thalamus and cortex. These studies have shown that parkinsonism is associated with increased activity in the basal ganglia output nuclei, along with increases in burst discharges, oscillatory firing and synchronous firing patterns throughout the basal ganglia. Computational approaches have the potential to play an important role in the interpretation of these data. Such efforts can provide a formalized view of neuronal interactions in the network of connections between the basal ganglia, thalamus, and cortex, allow for the exploration of possible contributions of particular network components to parkinsonism, and potentially result in new conceptual frameworks and hypotheses that can be subjected to biological testing. It has proven very difficult, however, to integrate the wealth of the experimental findings into coherent models of the disease. In this review, we provide an overview of the abnormalities in neuronal activity that have been associated with parkinsonism. Subsequently, we discuss some particular efforts to model the pathophysiologic mechanisms that may link abnormal basal ganglia activity to the cardinal parkinsonian motor signs and may help to explain the mechanisms underlying the therapeutic efficacy of deep brain stimulation for Parkinson's disease. We emphasize the logical structure of these computational studies, making clear the assumptions from which they proceed and the consequences and predictions that follow from these assumptions.
Interaction of<i>LEAFY</i>,<i>AGAMOUS</i>and<i>TERMINAL FLOWER1</i>in maintaining floral meristem identity in<i>Arabidopsis</i> (2002)

The Arabidopsis transcription factor LEAFY acts upstream of homeotic genes such as AGAMOUS to confer floral identity on meristems that arise after the transition to reproductive development. Compared to the genetic circuitry regulating the establishment of floral meristem identity, little is known about its maintenance. Previous experiments with leafy heterozygous plants and agamous mutants grown in conditions that reduce the floral inductive stimulus have shown that both genes are required to prevent reversion of floral to inflorescence meristems. Here, we present evidence that LEAFY maintains floral meristem identity independently of AGAMOUS, and that the primary role of LEAFY is either direct repression of shoot identity genes or repression of an intermediate factor that activates shoot identity genes. The latter conclusions were deduced from the phenotypes conferred by a gain-of-function transgene, LEAFY:VP16, that appears to act as a dominant negative, or antimorphic, allele during maintenance of floral meristem identity. These observations contrast with previous findings that LEAFY acts as a direct activator of floral homeotic genes, supporting the hypothesis that the transcriptional activity of LEAFY is dependent on specific co-regulators.
Effect of high dose corticosteroids alone or combined with other drugs on survival in septic shock. (1985)

The effect of high dose corticosteroids on survival has been studied in a limited number of canine septic shock models which are reviewed in this presentation. Following injection of live bacteria neither methylprednisolone, nor gentamicin but a combination improved survival. Methylprednisolone increased survival following a slow but not a bolus infusion of endotoxin. In a recent study the effects of short term treatment with methylprednisolone, naloxone and ibuprofen were studied in endotoxin shock. All control animals died within 36 hours. Five of 9 dogs receiving the combination methylprednisolone, naloxone and ibuprofen were permanent survivors. The combined treatment with methylprednisolone and ibuprofen also increased survival. Dogs treated with methylprednisolone alone did not differ significantly from controls. It is concluded that methylprednisolone alone has no significant effect on survival in septic shock, but seems to be an important therapeutic factor to achieve increased survival.
Deep eutectic solvents as highly active catalysts for the fast and mild glycolysis of poly(ethylene terephthalate)(PET) (2015)

Urea/metal salt DESs can catalyze PET degradation into a monomer with high selectivity in a short time under mild conditions.
Lithium protects cultured neurons against β‐amyloid‐induced neurodegeneration (1999)

The deposition of beta-amyloid peptide (A beta), the hyperphosphorylation of tau protein and the death of neurons in certain brain regions are characteristic features of Alzheimer's disease. It has been proposed that the accumulation of aggregates of A beta is the trigger of neurodegeneration in this disease. In support of this view, several studies have demonstrated that the treatment of cultured neurons with A beta leads to the hyperphosphorylation of tau protein and neuronal cell death. Here we report that lithium prevents the enhanced phosphorylation of tau protein at the sites recognized by antibodies Tau-1 and PHF-1 which occurs when cultured rat cortical neurons are incubated with A beta. Interestingly, lithium also significantly protects cultured neurons from A beta-induced cell death. These results raise the possibility of using chronic lithium treatment for the therapy of Alzheimer's disease.
Antioxidant and Cholesterol-reducing Properties of Enterococcus gallinarum m661 (2013)

An enterococcal strain named as m661 was isolated from porcine gastric juice.Strain m661 was found to be capable of producing lactic acid,Gram-positive and catalase-negative.Based on phylogenetic analysis,cellular and colonial morphology and biochemical test,m661 was identified as Enterococcus gallinarum.This strain had antioxidant potential,and its scavenging activity against hydroxyl,DPPH and superoxide anion radicals was in a cell density-dependent manner.At a cell density of 5 × 108 CFU/mL,the scavenging rates of stain m661 against hydroxyl,DPPH,and superoxide anion radicals were 1.58%,36.6% and 57.1%,respectively.In addition,m661 was found to reduce cholesterol by 34.4% in the culture medium and produce 89.9 mg/L phenyllactic acid,suggesting excellent health-benefiting effects.The results of this study demonstrate that strain m661 has great potential to be developed as a new probiotic resource.
Les démocraties latines de l'Amérique, F. Garcia Calderón. (1913)

Martin Ch. A. Les démocraties latines de l'Amérique, F. Garcia Calderón. In: Journal de la Société des Américanistes. Tome 10 n°1, 1913. pp. 291-293.
Validation of Component-Based Software with a Customer Centric Domain Level Approach (2007)

End user testing for higher-order software compliance becomes an issue of increasing importance with compositional reuse of software artifacts. Few if any existing approaches discuss the validation of higher-order domain aspects in this context. The proposed method derives testable validation scenarios directly from a customer domain model by abstraction, reduction and inclusion for critical coverage. The resulting branch-free validation scenarios are used as references to validate suppliers' software specifications against. Advantages of the method are first the embedding into a clear business model, second test oracles that originate from the business domains and thus are independent from models within the software development process, and third the early availability of validation results in the development cycle, before the software itself is available
Environmental compliance certificate of ZAMSURECO for the proposed Salug-Daku Mini-Hydro Power Plant Project (2007)

The objective of the Additional Financing for Rural Power Project for Philippines is to render better and effective to its consumer members, establishes the 10 MVA power sub-station to primarily solve the problem of power shortage. Negative measures include: loss of vegetation due to land clearing and backfilling, disturbance of wildlife within the influence area due to noise and other construction activities, generation of soil waste, air pollution, increase in the incidence of crime and accidents, erosion from exposed cuts and landslides due to earthmoving and excavation activities, and pollution of nearby water body due to improper disposal of construction wastes. Mitigation measures include: (a) set-up temporary disposal mechanism within the construction area and properly dispose the generated solid wastes; (b) limit land clearing as much as possible; (c) provide temporary fencing to vegetation that will be retained; (d) re-establish or stimulate the habitat of affected wildlife in another suitable area; (e) segregation of recyclable materials; and (f) schedule noise construction activities during day time.
Total Synthesis of the Didemnins; IV.<sup>1</sup>Synthesis of the Peptolide Ring and Construction of the Side Chain (1991)

A total synthesis of didemnins A, B, and C (1-3) which enables these highly cytotoxic cyclopeptides to be prepared in decigram amounts is described. The ß-keto acid unit (hydroxyisovaleryl)propionic acid derivative (Hip, 6) was prepared by acylation of dibenzyl methylmalonate and subsequent cleavage of the benzyl groups by the action of boron trichloride. The free ß-keto acid 6 was activated by the DCC method and allowed to react with the leucine ester 7 to furnish the amide 8. Activation, deprotection, and ring closure of the linear peptide 19 by means of the pentafluorophenyl ester method in a two-phase system gave rise to the didemnin ring skeleton in 75% yield within a few minutes. The respective side chains were then attached to the didemnin ring easily and in high yields by activation of Z-(R)-N-methylleucine as its 3-cyano-2-pyridylthiol ester followed by reaction with Z-lactylproline chloride and Z-lactic acid chloride.
Nitric oxide donors and the skin: useful therapeutic agents? (2003)

Nitric oxide (NO) is involved in several processes in the skin, including wound healing, pigmentation and regulation of apoptosis. Pharmacological intervention with NO donors appears to be a promising treatment for conditions such as diabetic ulceration. In this issue of Clinical Science, Khan et al. describe the synthesis of a number of NO donors, based on nitrosylated thiosugars, structural changes in which altered the rate of NO release. The in vivo behaviour of one of the donors was then measured by laser Doppler blood flow measurements on the skin. The ability to tailor the pharmacokinetic behaviour of these drugs should be of interest in developing NO donors for clinical use.
Protein‐kinase‐C iso‐enzymes support DNA synthesis and cell survival in colorectal‐tumor cells (1999)

Protein-kinase-C signalling has been blocked in colorectal tumor cells by kinase inhibitors, by TPA down-regulation or by exposure to anti-sense oligonucleotides.This resulted in growth inhibition in all cell lines used.The kinase inhibitors H7 and calphostin induced apoptosis, demonstrated by the appearance of cells with characteristically condensed chromatin and the induction of stand-breaks in the DNA.A cell-deathinducing concentration of 15 µg/ml H7 down-regulated the bcl-2 levels after 9 hr, while bak levels were not affected.Go ¨6976,-an inhibitor of Ca ؉؉ -dependent PKC iso-enzymes, was not active in growth inhibition or induction of apoptosis.Analysis of DNA synthesis in inhibitor-treated cultures indicated that H7 caused strong inhibition in all cell lines, while the more specific inhibitor calphostin was effective only in VACO235 adenoma cells.When down-regulation by TPA or anti-sense oligonucleotides was used to block PKC, effects on cell numbers were smaller and delayed.However, induction of apoptosis was significantly increased in SW480 carcinoma cells 4 days after exposure to anti-⑀ and anti-oligonucleotides in SW480 and T84 carcinoma cells.Apoptosis was preceeded by loss of PKC protein and of bcl-2 from day 1 after addition of the oligonucleotides.In VACO235 adenoma cells, no induction of apoptosis could be observed when anti-⑀ and anti-oligonucleotides were used.On the other hand, the adenoma cells were more responsive to anti-␣ and anti-␤ oligonucleotides, which strongly inhibited DNA-synthesis 3 days after addition to the culture medium.Our results indicate that the Ca ؉؉ -dependent PKCs ␣ and ␤ are involved in proliferation signals, while the Ca ؉؉ -independent PKCs ⑀ and are involved in survival pathways of colorectal tumor cells.
Separation of red blood cells at high volume concentration under low centrifugal accelerations (1988)

The separation process of blood and RBC suspensions in a hematocrit range between 0.3-0.7 was investigated with a centrifuge allowed to run at low accelerations (100 xg-1000 xg). The position of the interface between the supernatant of plasma and the RBC column was continuously recorded by a new optoelectronic measuring system. The separation process could be mathematically described by an exponential decrease of the cell column approaching a final packing. At a given centrifugal acceleration the time constant is influenced by hematocrit, aggregation, deformation and plasma viscosity. The final packing depends linearly on the starting hematocrit (0.3-0.7) and can be used as a measure of deformability.
Assessment of the Corvac Blood Collection Tube for Drug Specimen Processing (1986)

The Corvac integrated serum separator tube (TM Monoject Scientific) has been examined for use in processing specimens for drug analysis. This study was undertaken because the SST serum separator tube (Becton-Dickinson) was shown to cause decreases in drug levels of lidocaine, pentobarbital, and phenytoin, which were dependent upon blood volume and time of contact with the gel. Pools of donor blood were spiked with lidocaine or phenytoin and processed in Corvac tubes and red top tubes. Lidocaine levels were depressed by 5.2% when Corvac was used. Phenytoin levels were unchanged. The lidocaine depression was independent of blood volume, time of gel contact, or tube lot number. Paired patient specimens for quinidine, lidocaine, or theophylline analysis were obtained in Corvac and red top tubes. Lidocaine levels were depressed by 6.0% (p less than 0.01). Quinidine and theophylline levels were unchanged. All drug analyses were carried out using the Syva enzyme multiplied immunoassay technique. The Corvac tube has been shown to cause decreased levels of lidocaine but not of the other drugs tested. Corvac (silicone gel) results differ from SST (polyester gel) results because of the different construction of these tubes.
A new One-time Password Method (2013)

One-Time Passwords (OTP) can provide complete protection of the login-time authentication mechanism against replay attacks. In this paper, we propose TSOTP: a new effective simple OTP method that generates a unique passcode for each use. The calculation uses both time stamps and sequence numbers. A two-factor authentication prototype for mobile phones using this method has been developed and has been used in practice for a year.
In Vivo Assessment of Trabecular Smooth Muscle Tone, its Application in Pharmaco-Cavernosometry and Analysis of Intracavernous Pressure Determinants (1995)

A pharmaco-cavernosometry based clinical study was designed to define hemodynamic parameters consistent with complete trabecular smooth muscle relaxation, establish a methodology for overcoming incomplete trabecular smooth muscle relaxation, and determine under controlled conditions the contribution of venous outflow and arterial inflow to the steady-state equilibrium intracavernous pressure. Flow-pressure relationships were analyzed in 21 patients each of whom was assumed to have complete smooth muscle relaxation by virtue of the full, rigid and maintained erectile response following intracavernous vasodilator administration, which required intracavernous adrenergic agonists to achieve detumescence. Flow-to-maintain values increased linearly with intracavernous pressure while venous outflow resistance values were high and constant. Based on these relationships, trabecular smooth muscle tone was assessed in 123 impotent patients. In 14%, 63% and 14% of the patients (112 of 123 overall), respectively, 1, 2 and 3 doses of vasoactive agents were required to achieve hemodynamic relationships consistent with complete trabecular smooth muscle relaxation. In 9% of the patients such hemodynamic relationships were unable to be reached. In the 112 patients the influence of different engineering based measures of corporeal veno-occlusive function, including flow-to-maintain, pressure decay, venous outflow resistance and corporeal capacitance, was analyzed against the spectrum of equilibrium steady-state intracavernous pressures. Two distinct equilibrium pressure groups were identified reflecting different capacitance states: pressures greater than 60 mm. Hg (associated with low capacitance values) and pressures less than 50 mm. Hg (associated with high capacitance values), with pressures 50 to 59 mm. Hg representing a hemodynamic transition zone. When analyzed during complete trabecular smooth muscle relaxation, corporeal veno-occlusive hemodynamic variables in conjunction with cavernous arterial perfusion pressure determine the steady-state equilibrium intracavernous pressure. Failure to assess corporeal veno-occlusive function under such conditions will overestimate the degree of suspected corporeal structural disease.