gebhart/patrepeval huggingface dataset:
Quantitative differential effects of rhodamine 123 on normal cells and human colon cancer cells by magnetic resonance spectroscopy. (1993)

Rhodamine 123 is a lipophilic cationic compound that is selectively taken up by cancer cell mitochondria. This compound is toxic to epithelial cancer cells in vitro and displays significant anticancer activity in vivo. However, the mechanism of action of rhodamine 123 in intact, actively metabolizing cell preparations is unknown. We have used 31P- and 13C-nuclear magnetic resonance spectroscopy to quantitatively characterize how rhodamine 123 affects the energetics of human colon cancer cells (HCT-116) and spontaneously immortalized normal epithelial cells (CV-1). Rhodamine 123 differentially altered the phosphorus and glucose metabolism of HCT-116 and CV-1 cells. 31P-nuclear magnetic resonance detected mitochondrial poisoning in the HCT-116 human colon cancer cell line in its early stages after selective uptake of rhodamine 123. When we compared administration of rhodamine 123 and [1-C13]glucose to administration of [1-C13]glucose alone in the HCT-116 cells, we noted a marked decrease in intracellular pH to 6.7 +/- 0.06 (mean +/- SD) units, a 2.2-fold increase in lactate production, and a 1.8-fold increase in glucose consumption after 10 h. In addition, we found a 2-fold rise in intracellular free magnesium 12 h after rhodamine 123 administration. These results suggest that when rhodamine 123 inhibits mitochondrial ATP production, it initially stimulates cytoplasmic glycolysis in an attempt to maintain cellular energy demands. The marked fall in intracellular pH and rise in intracellular free magnesium after administration of rhodamine 123 may inhibit activity of several glycolytic enzymes: this effect would inhibit cytoplasmic ATP generation and interfere with multiple cell enzymatic processes, leading to cell death. The CV-1 cells showed no change in intracellular pH, intracellular free magnesium, or magnesium-bound ATP levels over the 24-h period following rhodamine 123 administration. Rhodamine 123 also failed to alter glucose utilization and lactate production levels significantly in the CV-1 cells. These results prove the usefulness of 31P- and 13C-nuclear magnetic resonance spectroscopy for quantifying differing effects of rhodamine 123 on the high energy phosphate metabolism and glucose metabolism of HCT-116 and CV-1 cells.
Beam breakup integral measurement on high-power laser chains (2007)

We experimentally demonstrate the efficiency of a single-shot method to measure the beam breakup integral (B) accumulated across a high power chain. The technique uses spectrally shaped strongly chirped femtosecond pulses and takes advantage of time-to-spectral coupling generated by nonlinear effects. We performed B measurements on regenerative amplifiers (Ti:sapphire) and on the ALISE 200 J facility currently installed at CEA-CESTA (France).
Keratinocyte migration requires α2β1 integrin-mediated interaction with the laminin 5 γ2 chain (2001)

Keratinocyte migration is an absolute requirement for correct epithelialization during the process of wound healing. This process requires changes in extracellular matrix ligand expression as well as changes in ligand-binding affinity of the corresponding cellular integrins. In this study, we attempt to understand the role of laminin 5 in migration by investigating the integrin-mediated interactions of migrating keratinocytes with their newly synthesized laminin 5. We chose to induce migration of freshly isolated NHK in vitro by exposing them to TGF-beta1 which, in addition to promoting epithelial cell migration, is also known to prevent cell proliferation. This important feature allowed the study to be focused on cell migration without interfering with cell proliferation. We confirm that keratinocyte migration on plastic, fibronectin or collagen IV substrates requires endogenous laminin 5 deposition, which is predominantly detected under its unprocessed form. Despite a crucial role for laminin 5 in migration, we show that this process is accompanied by a significant decrease in adhesion to purified laminin 5. Moreover, we provide evidence that the alpha2beta1 integrin interaction with newly synthesized laminin 5 renders the cells more adherent and retards migration. Conversely, we provide evidence that the alpha2beta1 integrin-laminin 5 interaction is absolutely required for keratinocyte migration and that the alpha2beta1 integrin is responsible for cell spreading on laminin 5. Finally, we demonstrate that the alpha2beta1 integrin binding to laminin 5 occurs within the short arm of the gamma2 subunit.
Aberrant MCT4 and GLUT1 expression is correlated with early recurrence and poor prognosis of hepatocellular carcinoma after hepatectomy (2018)

The tumor microenvironment is a key determinant of cancer cell biology. The microenvironment is a complex mixture of tumor cells, stromal cells, and proteins, extracellular matrix, oxygen tension, and pH levels surrounding the cells that regulate the tumor progress. This study identified the prognostic factors associated with hepatocellular carcinoma (HCC) and MCT4 and GLUT1 expression levels in HCC specimens. In this study, we analyzed MCT4 and GLUT1 expression levels in tissue samples from 213 patients with HCC by immunohistochemical analyses and in HCC tumor tissues and matched adjacent nonneoplastic tissues by quantitative real-time PCR. We conducted a prognostic analysis of the overall survival (OS) and time to recurrence (TTR) using immunoreactivity and other common clinical and pathological parameters. All variables with prognostic impact were further analyzed by multivariate analysis. We found that MCT4 and GLUT1 expression levels were significantly higher in tumor tissues than in adjacent nontumor tissues, and they were positively correlated with tumor size. Survival analysis showed that patients with high expression levels of MCT4 or GLUT1 had a poor OS and TTR. In patients with HCC, MCT4 expression was an independent negative prognostic factor for OS (hazard ratio [HR] = 1.617; 95% confidence interval [CI] = 1.102-2.374; P = 0.014), and metabolic indicators were independent prognostic factors for OS (HR = 1.617, 95% CI = 1.102-2.374, P = 0.006) and TTR (HR = 1.348, 95% CI = 1.079-1.685, P = 0.009). Interestingly, patients with positive metabolic indicator expression in tumor cells had a significantly shorter OS and earlier TTR than those with negative metabolic indicator expression in tumor cells in the ≤5 cm and >5 cm subgroups. In summary, using the expression of MCT4 and GLUT1 and their metabolic parameters to determine the metabolic status of tumors is promising for predicting the prognosis of patients with HCC.
The effects of phosphatidylserine on endocrine response to moderate intensity exercise (2008)

The findings suggest that PS is an effective supplement for combating exercise-induced stress and preventing the physiological deterioration that can accompany too much exercise. PS supplementation promotes a desired hormonal status for athletes by blunting increases in cortisol levels.
Site-Specific Labeling of Proteins Using Unnatural Amino Acids. (2019)

Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.
Vulnerability evaluation of speaker verification under voice conversion spoofing: the effect of text constraints (2013)

Voice conversion, a technique to change one's voice to sound like that of another, poses a threat to even high performance speaker verification system.Vulnerability of text-independent speaker verification systems under spoofing attack, using statistical voice conversion technique, was evaluated and confirmed in our previous work.In this paper, we further extend the study to text-dependent speaker verification systems.In particular, we compare both joint density Gaussian mixture model (JD-GMM) and unit-selection (US) spoofing methods and, for the first time, the performances of text-independent and text-dependent speaker verification systems in a single study.We conduct the experiments using RSR2015 database which is recorded using multiple mobile devices.The experimental results indicate that text-dependent speaker verification system tolerates spoofing attacks better than the text-independent counterpart.
Fast plane segmentation with line primitives for RGB-D sensor (2016)

This article presents a fast and robust plane segmentation approach for RGB-D type sensor, which detects plane candidates by line segments extracted from 2-D scanline projected from row or column points. It neither requires high computation to calculate local normals for the entire point cloud as most of approaches do nor randomly chooses plane candidates such as RANSAC-like approaches. First, a line extraction algorithm is utilized to extract line segments. Second, the plane candidates are detected by estimating local normal of points lying on line segments. Finally, the plane having most inlier is recursively extracted from the plane candidates as the result plane. Experiments were conducted with different data sets and the segmentation performances were evaluated quantitatively and qualitatively. We demonstrated the efficiency and robustness of our proposed approach, especially in the none plane scenario, the approach needs little computational cost.
Maintenance Therapy in Advanced Ovarian Cancer: Progression-Free Survival and Clinical Benefit (2003)

Article Tools EDITORIALS Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2003.03.039 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 12829660 Maintenance Therapy in Advanced Ovarian Cancer: Progression-Free Survival and Clinical Benefit Robert F. OzolsxRobert F. OzolsSearch for articles by this author Show More Fox Chase Cancer Center, Philadelphia, PA https://doi.org/10.1200/JCO.2003.03.039 First Page Full Text PDF Figures and Tables © 2003 by American Society of Clinical Oncology
Phase-defined complete sequencing of the HLA genes by next-generation sequencing (2013)

Abstract Background The human leukocyte antigen (HLA) region, the 3.8-Mb segment of the human genome at 6p21, has been associated with more than 100 different diseases, mostly autoimmune diseases. Due to the complex nature of HLA genes, there are difficulties in elucidating complete HLA gene sequences especially HLA gene haplotype structures by the conventional sequencing method. We propose a novel, accurate, and cost-effective method for generating phase-defined complete sequencing of HLA genes by using indexed multiplex next generation sequencing. Results A total of 33 HLA homozygous samples, 11 HLA heterozygous samples, and 3 parents-child families were subjected to phase-defined HLA gene sequencing. We applied long-range PCR to amplify six HLA genes ( HLA-A, -C, -B, DRB1, -DQB1 , and –DPB1 ) followed by transposase-based library construction and multiplex sequencing with the MiSeq sequencer. Paired-end reads (2 × 250 bp) derived from the sequencer were aligned to the six HLA gene segments of UCSC hg19 allowing at most 80 bases mismatch. For HLA homozygous samples, the six amplicons of an individual were pooled and simultaneously sequenced and mapped as an individual-tagging method. The paired-end reads were aligned to corresponding genes of UCSC hg19 and unambiguous, continuous sequences were obtained. For HLA heterozygous samples, each amplicon was separately sequenced and mapped as a gene-tagging method. After alignments, we detected informative paired-end reads harboring SNVs on both forward and reverse reads that are used to separate two chromosomes and to generate two phase-defined sequences in an individual. Consequently, we were able to determine the phase-defined HLA gene sequences from promoter to 3′-UTR and assign up to 8-digit HLA allele numbers, regardless of whether the alleles are rare or novel. Parent–child trio-based sequencing validated our sequencing and phasing methods. Conclusions Our protocol generated phased-defined sequences of the entire HLA genes, resulting in high resolution HLA typing and new allele detection.
Don't mess with Texas (2014)

If you're running through the Lone Star State, don't skimp on the pre-trip inspections. No other state records a higher percentage of maintenance violations.
Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset (2021)

The study was funded by a generous donation from The Hour Glass to support COVID-19 research.
Role of sialyl Lewis(x) in total hepatic ischemia and reperfusion. (1996)

The SLe(x) oligosaccharide analog, CY-1503, had an important protective role in hepatic ischemia and reperfusion injury. Modulation of SLe(x) in the neutrophil decreased the adhesion of polymorphonuclear cells and their subsequent migration after hepatic ischemia and reperfusion.
Automatic title generation for spoken broadcast news (2001)

In this paper, we implemented a set of title generation methods using training set of 21190 news stories and evaluated them on an independent test corpus of 1006 broadcast news documents, comparing the results over manual transcription to the results over automatically recognized speech. We use both F1 and the average number of correct title words in the correct order as metric. Overall, the results show that title generation for speech recognized news documents is possible at a level approaching the accuracy of titles generated for perfect text transcriptions.
Spectral characteristics of vacuum ultraviolet preionization sources for CO<sub>2</sub>lasers (1978)

Results are presented of a comparative study of the radiation spectra of spark and surface discharges in the 114–200 nm range. It is shown that both complete and incomplete surface discharges are a more efficient source of ultraviolet radiation for preionization of the active volume of CO2 lasers than spark discharges. This is attributed to the possibility of concentrating the radiation energy in the required spectral range and of locating the discharge plasma in the optimal position with respect to the laser active volume. An analysis of the time behavior of the radiation intensity of the most characteristic spectral lines showed that the changes in the spectrum of an incomplete surface discharge with polarity reversal of the supply pulse were associated with the surface-discharge-formation mechanism.
Neural Inverse Knitting: From Images to Manufacturing Instructions (2019)

Motivated by the recent potential of mass customization brought by whole-garment knitting machines, we introduce the new problem of automatic machine instruction generation using a single image of the desired physical product, which we apply to machine knitting. We propose to tackle this problem by directly learning to synthesize regular machine instructions from real images. We create a cured dataset of real samples with their instruction counterpart and propose to use synthetic images to augment it in a novel way. We theoretically motivate our data mixing framework and show empirical results suggesting that making real images look more synthetic is beneficial in our problem setup.
Ammonia affects performance and thermoregulation of male broiler chickens (2004)

The effects of various atmospheric ammonia concentrations (X S.E.; 16 2; 28 3; 39 4; and 54 5 ppm) at high ambient temperature (T a = 32 C) and relative humidity (rh = 60-65%) on the performance and thermoregulation of male broiler chickens were studied. Body weight declined significantly and proportionally with increasing ammonia concentration in the air. The decline in body weight coincided with a similar pattern in feed intake. Feed efficiency did not differ significantly among treatments, but the broilers exposed to the lowest concentration of ammonia showed the highest feed efficiency. Chickens exposed to the lowest ammonia concentrations regulated T b at significantly lower levels (41.5 C at 16 ppm and 41.9 C at 28 ppm) than those at the highest concentrations (42.3 C at 39 and 54 ppm). Arterial pH increased with increasing ammonia concentration and was similar in the upper 2 concentrations of ammonia. A similar but opposite trend was found in the partial pressure of arterial CO 2 . Plasma triiodothyronine (T 3 ) concentration did not differ significantly among treatments. It can be concluded that exposure to increased ammonia concentrations impairs broiler performance. It may also be suggested that ammonia can affect the ability of the chickens to control T b effectively. ammonia / broiler / performance / body temperature / triiodothyronine Rsum -L'ammoniac rduit les performances et affecte la thermorgulation des poulets de chair mles. Les effets de diffrentes concentrations d'ammoniac dans l'air (16 2 ; 28 3 ; 39 4 et 54 5 ppm) pour une temprature ambiante leve (T a = 32 C) et une hygromtrie de 60-65 %, ont t mesurs sur les performances et la thermorgulation des poulets de chair mles. Le poids vif diminue significativement et proportionnellement aux concentrations croissantes d'ammoniac dans l'air. Cet effet concide avec les variations de l'ingr alimentaire. L'efficacit alimentaire n'a pas t significativement diffrente d'un traitement l'autre, mais les poulets exposs la plus faible concentration d'ammoniac ont prsent la meilleure efficacit alimentaire. La temprature corporelle (T b ) des poulets exposs aux plus faibles concentrations d'ammoniac a t rgule un niveau plus bas (41,5 C 16 ppm et 41,9 C 28 ppm) que celle des poulets levs aux hautes concentrations d'ammoniac (42,3 C 39 et 54 ppm). Le pH artriel a augment avec la concentration d'ammoniac dans l'air en restant similaire pour les deux doses les plus leves. Une volution oppose a t observe pour la pression partielle de CO 2 artriel. La concentration plasmatique en triiodothyronine (T 3 ) ne diffre pas significativement entre les traitements. On peut donc conclure que l'exposition des concentrations croissantes d'ammoniac dans l'air rduit les performances des poulets.
Guided-waves technique for inspecting the health of wall-covered building risers (2015)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Peter W. Tse, J. M. Chen, X. Wan; Guided-waves technique for inspecting the health of wall-covered building risers. AIP Conference Proceedings 31 March 2015; 1650 (1): 676–685. https://doi.org/10.1063/1.4914668 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Assembly of the Murine Leukemia Virus Is Directed towards Sites of Cell–Cell Contact (2009)

We have investigated the underlying mechanism by which direct cell-cell contact enhances the efficiency of cell-to-cell transmission of retroviruses. Applying 4D imaging to a model retrovirus, the murine leukemia virus, we directly monitor and quantify sequential assembly, release, and transmission events for individual viral particles as they happen in living cells. We demonstrate that de novo assembly is highly polarized towards zones of cell-cell contact. Viruses assembled approximately 10-fold more frequently at zones of cell contact with no change in assembly kinetics. Gag proteins were drawn to adhesive zones formed by viral Env glycoprotein and its cognate receptor to promote virus assembly at cell-cell contact. This process was dependent on the cytoplasmic tail of viral Env. Env lacking the cytoplasmic tail while still allowing for contact formation, failed to direct virus assembly towards contact sites. Our data describe a novel role for the viral Env glycoprotein in establishing cell-cell adhesion and polarization of assembly prior to becoming a fusion protein to allow virus entry into cells.
Cardiovascular risk factors and probability for cardiovascular events in HIV-infected patients: part I. Differences due to the acquisition of HIV-infection. (2003)

The cardiovascular risk profile differs between subgroups of HIV-infected individuals, leading to significant higher probability of cardiovascular events in group 1 subjects. The risk of premature atherosclerosis by HIV-infected individuals and therapeutic options remains to be established.
Resonant cavity-enhanced (RCE) photodetectors (1991)

The photosensitivity characteristics of resonant cavity-enhanced (RCE) photodetectors are investigated. The photodetectors were formed by integrating the active absorption region into a resonant cavity composed of top and bottom (buried) mirrors. A general expression for quantum efficiency for RCE photodetectors was derived taking the external losses into account. Drastic enhancement in quantum efficiency is demonstrated at resonant wavelengths for a high quality factor Q cavity with a very thin absorption layer. An improvement by a factor of four in the bandwidth-efficiency product for RCE p-i-n detectors is predicted. Molecular beam epitaxy grown RCE-heterojunction phototransistors (RCE-HPT) were fabricated and measured demonstrating good agreement between experiment and theory.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Spectrum of C9orf72-mediated Neurodegeneration and Amyotrophic Lateral Sclerosis (2015)

The discovery that a hexanucleotide repeat expansion in C9orf72 is the most numerous genetic variant of both amyotrophic lateral sclerosis and frontotemporal dementia has opened a rapidly growing field, which may provide long hoped for advances in the understanding and treatment of these devastating diseases. In this review we describe the various phenotypes, clinical and pathological, associated with expansion of C9orf72, which go beyond amyotrophic lateral sclerosis and frontotemporal dementia to include neurodegeneration more broadly. Next we take a step back and summarize the current understanding of the C9orf72 expansion and its protein products at a molecular level. Three mechanisms are prominent: toxicity mediated directly by RNA transcribed from the repeat; toxicity mediated by dipeptide repeat proteins translated from the repeat sequence; and haploinsufficiency resulting from reduced transcription of the C9orf72 exonic sequence. A series of exciting advances have recently described how dipeptide repeat proteins might interfere with the normal role of the nucleolus in maturation of RNA binding proteins and in production of ribosomes. Importantly, these mechanisms are unlikely to be mutually exclusive. We draw attention to the fact that clinical and pathological similarities to other genetic variants without a repeat expansion must not be overlooked in ascribing a pathogenic mechanism to C9orf72-disease. Finally, with a view to impact on patient care, we discuss current practice with respect to genetic screening in patients with and without a family history of disease, and the most promising developments towards therapy that have been reported to date.