gebhart/patrepeval huggingface dataset:
Intracellular accumulation of the amyloidogenic L68Q variant of human cystatin C in NIH/3T3 cells. (1998)

The cellular transport of human cystatin C is impeded by the pathogenic amino acid substitution Leu68-->Gln. The resulting intracellular accumulation and increased localised concentration of L68Q cystatin C might be an important event in the molecular pathophysiology of amyloid formation and brain haemorrhage in patients with HCCAA.
Learning about Complexity with Modular Robots (2008)

We present progress with roBlocks, a reconfigurable modular robotic system for education. Children snap together small, magnetic, heterogeneous modules to create larger, more complex robotic constructions. The design of the system is described and the algorithms that handle data transfer and manipulation are explained. Users tend to begin exploring the system through a series of simple robot patterns but quickly progress to more involved constructions. Many years before they learn formally about hierarchy and modularity, children can develop intuitions about these concepts by designing modular robots. Additionally, young users often spontaneously engage in creative debugging practices.
Combustion of Uniformly Sized Glassy Carbon Particles (1987)

Abstract Abstract-A method for production of spherical particles of glassy carbon has been developed. The panicles exhibit some differences in surface structure, but are otherwise uniform in size and shape. In drop-tube combustion experiments at wall temperatures ranging from 1200 K to 1600 K, some panicles ignited and burned rapidly, reaching temperatures several hundred degrees higher than the wall temperature, while o thers burned as much as two orders of magnitude more slowly at temperatures only slightly higher than that of the reactor wall. In spite of these differences in ignition behavior, the particles burned with an intrinsic reaction rate that was in close agreement with the Nagle and Strickland-Constable rate for pyrolytic graphite as well as rates previously measured for carbon black and coal char oxidation. X-ray diffraction measurements show that graphitization occurs rapidly in a hot oxidizing atmosphere. Rapid anneal ing of the carbon structure might account for the similarity of the high temperature oxidation rates of carbons from different sources.
Image restoration in computed tomography: restoration of experimental CT images (1992)

It is pointed out that to restore experimental computed tomography (CT) images which have been blurred by a spatially variant point spread function (PSF), a quadrant symmetry method which simultaneously optimizes storage requirements for the estimated PSFs and computational speed is used. The quadrant symmetry approach required less than 0.579 Megawords of storage for 9x9 pixel spatially variant PSFs in a 256x256 pixel image, allowing image restoration in Cartesian coordinates. A locally adaptive restoration method formulated using a noise prewhitening filter derived from the measured noise power spectrum, the conjugate gradient method was used to obtain a numerical solution. Restored images for a resolution phantom and for a cadaver femur are presented.
Evidence-based timelines for project retrospectives — A method for assessing requirements engineering in context (2012)

Effective requirements engineering (RE) can support efficient development of successful products. However, assessing and improving how RE supports its context, i.e. the development life cycle, is non-trivial since many different roles and factors are involved over a long period of time. Project retrospectives may support project teams in reflecting on how requirements are agreed upon and communicated throughout a project. However, time is rarely taken for group reflection after project completion. Furthermore, project events may be recalled differently due to memory bias. We propose supporting project retrospective meetings by providing prepared evidence-based timelines visualizing the project history. The method was designed and evaluated in collaboration with a large telecommunications company using action research with the goal of assessing RE within the full development life-cycle. The initial evaluation results show that the method may support project retrospectives through fact-based memory recall and by enabling efficient and factual group discussions of RE in the context of the project life-cycle. In addition, some areas for improvement of the method have been identified, e.g. strengthened focus on expected outcome and clearer visual separation of evidence types.
Effects of Fungicide Fenaminosulf and Plant Growth Regulator on Gall Formation of Zizania latifolia (2014)

In this research, the promotion of fungicide fenaminosulf and plant growth regulator in the gall formation had been produced by spraying fenaminosulf and plant growth regulator combinations at different growth development phases of Zizania latifolia ‘Longjiao 2'. After comparative and statistical analyzing of the plant heights, changes of endogenous hormone(IAA, ABA and GA3) in stem and yields of gall formation, it was found that the spraying of fenaminosulf with a certain concentration could signifi cantly promote the yields of gall formation in Z. latifolia, especially the spraying combinations of fenaminosulf(standard concentration) with nutrient solution and fenaminosulf(standard concentration). The promotions of plant growth regulator were also found, which was related to the different contents of plant growth regulator. Besides, the treatments of plant growth regulator could signifi cantly promote the early growth of plants, but little effect on the fi nal plants height. Using the methods of indirect ELISA, the contents of IAA, ABA and GA3 at the residual part of stem in Z. latifolia were compared and analysed. It was showed that the spraying of fenaminosulf could reduce the contents of ABA obviously, but there were no signifi cant infl uences on the contents of IAA and GA3. The reduction of ABA before the enlargement of Z. latifolia maybe relate to the yields of gall formation.
VirtPerf: A Performance Profiling Tool for Virtualized Environments (2011)

Several applications in the "physical'' world are being consolidated in "virtual'' environments using different virtualization technologies. An important criteria for this exercise is to understand potential resource requirements and performance levels achieved in virtual environments. Empirical evidence of these can be gotten by benchmarking the application's performance in a controlled manner in virtual environments. These measurements can be used for a variety of purposes from virtual machine capacity planning to building sophisticated performance models to predict performance for loads that cannot be practically tested. In this paper, we present VirtPerf, an integrated workload generator and measurement tool to capture resource utilization levels and performance metrics of applications executing under controlled circumstances in virtualized environments. The tool aims to provide comprehensive measurement-based analysis for applications in different virtualization settings. Additionally, a configurable workload generator can be used to stress and profile applications under different load conditions. We present the detailed design of VirtPerf and a comprehensive empirical study to demonstrate its correctness and capabilities.
Development of a CiFT Co-expression System for Functional Analysis of Genes in Citrus Flowers and Fruit (2009)

We have developed a CiFT co-expression system for the rapid evaluation of transgenic citrus flowers and fruit. In order to co-express a target gene and the CiFT gene, a CiFT co-expression vector was constructed by inserting the fragment containing a chimeric target gene into the binary vector harboring P35S::CiFT. This system was applied to metabolically engineer aroma components in trifoliate orange (Poncirus trifoliata L. Raf). In order to reduce the limonene level, a gene encoding limonene synthase (CitMTSE1) from satsuma mandarin (Citrus unshiu Marc.) was introduced into trifoliate orange in an anti-sense orientation with the CiFT co-expression vector. Transgenic plants flowered extremely early, and began to produce normal fruit within 2 years of Agrobacterium infection. Transcripts of transgenes were accumulated in reproductive tissues of transgenic plants, and endogenous transcripts for limonene synthase were reduced. The ratio of limonene to other monoterpenes was significantly decreased in flowers and fruit of transgenic plants. These results clearly indicated that the CiFT co-expression system is a useful tool for the functional analysis of genes in citrus flowers and fruit. Such analyses are important for several applications, including the metabolic engineering of aroma components in citrus.
From Modeling to Deployment of Component-Based Vehicular Distributed Real-Time Systems (2014)

We present complete model-and component based approach for the development of vehicular distributed real-time systems. Within this context, we model and timing analyze these systems using one of the state-of-the-practice modeling and timing analysis techniques that is implemented in the existing industrial model the Rubus Component Model and accompanying tool suite. As a proof of concept, we conduct a case study by developing an intelligent parking assist system which is a distributed real-time application from the vehicular domain. The case study shows various stages during the development such as modeling of software architecture, performing timing analysis, simulation, testing, automatic synthesis of code from the software architecture, deployment, and execution.
Pulse synchronizing dc-to-dc converters (1980)

The new dc-to-dc converter driven by the external pulse is presented. The principle is based on the synchronizing phenomenon in the transistor core multivibrator. There are the voltage feedback in the off interval and the current feedback in the on interval. The flux of the saturable core is changed along the minor hysteresis loop. The operation are explained by the aid of averaging method of the state space technique. The efficiency of this converter is inherently high and the construction is very simple. Discussions are given on the circuit as a switching regulator.
Evaluating passively shielded gradient coil configurations for optimal eddy current compensation (2010)

In magnetic resonance imaging, rapidly switching magnetic fields are used to spatially encode the signal. The temporal change of these fields induces eddy currents in nearby conducting structures of the scanner. These eddy currents, in turn, generate a secondary magnetic field that opposes and distorts the desired gradient field. Eddy current compensation methods are generally applied assuming that the primary and secondary magnetic field gradients possess similar spatial characteristics in the imaging volume (field matching). In this work an optimization method is used to deform the shape of the coil support and/or a highly conductive passive shield in order to improve the field matching and reduce the inductive coupling between the gradient coil and the passive shield. Using the residual field after eddy current compensation as the objective function, the coil support and/or conducting surfaces were deformed to obtain passively shielded x- and z-gradient coils with improved field matching and eddy current compensation. Assuming a single frequency, quasi-static simulation, it was demonstrated that the residual field was reduced up to 24 times by reshaping the coil and passive shield surfaces due to the improved field matching. However, using transient analyses we showed that in the case of the passively shielded x-gradient coil the residual field may only be reduced by five times from a cylindrical coil configuration. A bulge shape is created in the conducting surface as a mechanism of matching the field and at the same time reducing the mutual inductive coupling between the coil and the passive shield. An actively shielded coil with control over the magnetic field produced by the induced current was used as a reference coil that produces the minimal residual field. The actively shielded gradient coil produces minimal residual field for short and long pulses in the transient analyses.
Addition of plerixafor for <scp>CD</scp>34+ cell mobilization in six healthy stem cell donors ensured satisfactory grafts for transplantation (2013)

We have presented six cases with different causes leading to insufficient G-CSF mobilization in allogeneic donors and in which the administration of plerixafor just-in-time ensured a proper graft for transplantation.
In Vivo Assessment of the Trabecular Bone Microstructure of the Distal Radius Using a Compact MRI System (2009)

We repeatedly measured the trabecular bone (TB) microstructure of the distal radius in 5 healthy volunteers using a compact magnetic resonance (MR) imaging system to evaluate the reproducibility of the TB structural parameters. The compact system was specially developed with a 1.0-tesla permanent magnet for the distal radius in a previous study. We measured the distal radius using a 3-dimensional (3D), driven-equilibrium, spin-echo sequence (repetition time/echo time=80 ms/10 ms, number of excitations=1, field of view=76.8 mm x 57.6 mm x 16.0 mm, acquisition time=17 min, matrix size=512 x 384 x 32, voxel size=150 microm x 150 microm x 500 microm) and analyzed the acquired image datasets using the virtual bone biopsy system developed by the University of Pennsylvania. Root mean squares of the coefficients of variance expressed as percentages ranged from 2.9% to 14% for the TB structural parameters. Therefore, we concluded that this compact MR imaging system could be used to assess TB microstructure of the distal radius and provide results consistent with those using a whole-body MR imaging system and showing the promise of this system for evaluating bone quality in clinical settings.
Direct Determination of Trace Elements in Graphite Matrices Using Modulated Glow Discharge Atomic Absorption Spectrometry (1993)

The trace element concentrations in high-purity graphites were determined by atomic absorption spectrometry (AAS) with the use of a modulated glow discharge atomizer. Glow discharge power was modulated at SO Hz to enhance sensitivity and to permit time-resolved atomic absorption measurements. The influence of discharge operating parameters including current, pressure, and plasma sampling on the analytical performance of this technique was investigated. This analytical technique was evaluated in terms of stability, sensitivity, reproducibility, and accuracy. The method of standard additions was used to determine the impurity levels of the standard graphite matrix. Linear analytical calibration curves were constructed for four elements of interest in samples of nuclear-grade graphite. Detection limits for these elements were calculated to be in the tens of parts-per-billion range for graphite matrices.
Measuring the Contractile Forces of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes With Arrays of Microposts (2014)

Human stem cell-derived cardiomyocytes hold promise for heart repair, disease modeling, drug screening, and for studies of developmental biology. All of these applications can be improved by assessing the contractility of cardiomyocytes at the single cell level. We have developed an in vitro platform for assessing the contractile performance of stem cell-derived cardiomyocytes that is compatible with other common endpoints such as microscopy and molecular biology. Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were seeded onto elastomeric micropost arrays in order to characterize the contractile force, velocity, and power produced by these cells. We assessed contractile function by tracking the deflection of microposts beneath an individual hiPSC-CM with optical microscopy. Immunofluorescent staining of these cells was employed to assess their spread area, nucleation, and sarcomeric structure on the microposts. Following seeding of hiPSC-CMs onto microposts coated with fibronectin, laminin, and collagen IV, we found that hiPSC-CMs on laminin coatings demonstrated higher attachment, spread area, and contractile velocity than those seeded on fibronectin or collagen IV coatings. Under optimized conditions, hiPSC-CMs spread to an area of approximately 420 μm2, generated systolic forces of approximately 15 nN/cell, showed contraction and relaxation rates of 1.74 μm/s and 1.46 μm/s, respectively, and had a peak contraction power of 29 fW. Thus, elastomeric micropost arrays can be used to study the contractile strength and kinetics of hiPSC-CMs. This system should facilitate studies of hiPSC-CM maturation, disease modeling, and drug screens as well as fundamental studies of human cardiac contraction.
Effect of Age and Diagnosis on Survival of Older Patients Beginning Chronic Dialysis (1994)

Mortality rates of patients older than 55 years beginning chronic dialysis treatment increased dramatically as age at initiation of dialysis increased. Clinically meaningful survival data should prove useful to persons making decisions about the initiation of chronic dialysis.
Effect of glyphosate application timings and methods on glyphosate-resistant cotton (2004)

Under certain conditions, application of glyphosate to glyphosate-resistant (GR) cotton can lead to fruit shedding and yield reductions. Field studies were conducted at the Texas Agricultural Experiment Station using GR cotton, cv. ‘DeltaPine 5690RR’, to determine if application method and timing affect cotton fruit retention. Glyphosate at 1.12 kg ai ha −1 was precisely postdirected (PD), postdirected with 25% foliage coverage (PDFC), or applied over the top (OT) at the 8- or 18-leaf stage after an initial topical application of 1.12 kg ha −1 glyphosate at the four-leaf stage. In one of the years of this study, 8 PD, 18 PDFC, and 18 OT reduced yield. In 1999 and 2000, 8 PDFC and 8 OT applications of glyphosate caused yield loss, mainly due to lower mean boll weight. Glyphosate applied topically at the eight-leaf stage also affected the Position 1 boll retention throughout the plant in both years. Glyphosate contact with leaves and stems should be avoided when applying glyphosate after the four-leaf stage to prevent possible yield loss.
A new topology of direct AC to AC converter with soft-switching based on a MERS pulse link concept (2011)

This paper proposes a direct ac/ac converter without large smoothing capacitor. The first stage of the converter is a rectifier which generates a high frequency pulse voltage from an ac source. The second stage is a inverter which generates an ac voltage from the pulse voltage. The proposed converter does not have smoothing capacitor between the rectifier and inverter but has a drastically reduced capacitor size which is used for resonance in short switching cycle. Another advantage of the proposed converter is soft-switching operation of both the rectifier and inverter side. However, strict control of power flow is needed since there is no energy buffer between the rectifier and inverter. Thus, this paper also describes a basic control principle of the proposed converter and experimental results with a small scale setup.
Functionalized Graphene Oxide as a Nanocarrier in a Multienzyme Labeling Amplification Strategy for Ultrasensitive Electrochemical Immunoassay of Phosphorylated p53 (S392) (2011)

P53 phosphorylation plays an important role in many biological processes and might be used as a potential biomarker in clinical diagnoses. We report a new electrochemical immunosensor for ultrasensitive detection of phosphorylated p53 at Ser392 (phospho-p53(392)) based on graphene oxide (GO) as a nanocarrier in a multienzyme amplification strategy. Greatly enhanced sensitivity was achieved by using the bioconjugates featuring horseradish peroxidase (HRP) and p53(392) signal antibody (p53(392)Ab(2)) linked to functionalized GO (HRP-p53(392)Ab(2)-GO) at a high ratio of HRP/p53(392)Ab(2). After a sandwich immunoreaction, the HRP-p53(392)Ab(2)-GO captured onto the electrode surface produced an amplified electrocatalytic response by the reduction of enzymatically oxidized thionine in the presence of hydrogen peroxide. The increase of response current was proportional to the phospho-p53(392) concentration in the range of 0.02-2 nM with the detection limit of 0.01 nM, which was 10-fold lower than that of the traditional sandwich electrochemical measurement for p53(392). The amplified immunoassay developed in this work shows acceptable stability and reproducibility, and the assay results for phospho-p53(392) spiked in human plasma also show good recovery (92-103.8%). This simple and low-cost immunosensor shows great promise for detection of other phosphorylated proteins and clinical applications.
Applying chitosan to increase the fungal resistance of paper-based cultural relics. (2009)

Paper-based artworks and documents often suffer damage from the growth of fungi. Biological damage is mainly brought about by unsuitable environmental conditions and often results in discoloration and structural damage to the basic components of the materials or to the essential additive constituents. Mold growing on paper products is one kind of bio-deterioration; moldy stains on paper obviously decrease the life of rare books, paintings, and art crafts, and make them more difficult to preserve. Chitin, after cellulose, is the 2nd most abundant material in nature. Chitosan is a substance obtained by the deacetylation of chitin, the principal component of many living things, including crustaceans, insects, and fungi. Because of their superior properties and abundance, chitosan and chitin are extensively commercially utilized worldwide. Due to the fact that chitosan is a cationic polymer and a cationic polyelectrolyte, it shows superior antimicrobial properties. The purpose of this study was to investigate the feasibility of using chitosan as a possible material to increase the fungal resistance of paper-based cultural relics. Filter paper treated with 1.5% deacetylated chitosan showed the best fungal resistance effect; the higher the concentration of chitosan, the more superior was the fungal resistance effect obtained. At the same concentration, the higher the deacetylation of chitosan, the better the fungal resistance effect was. The application of chitosan to increase the fungal resistance of paper-based cultural relics can be a viable alternative conservation strategy for paper-based artifacts and archives.
High performance thin film bulk acoustic resonator covered with carbon nanotubes (2006)

This letter presents experimental results concerning a thin film bulk acoustic wave resonator realized on a thin GaN membrane and covered with a thin film of double walled carbon nanotube mixture. The quality factor was measured before and after the coating of the resonator with the nanotube thin film. The quality factor has increased more than ten times when the resonator was coated with nanotubes, due to their high elasticity modulus and low density, which confers a much higher acoustic impedance of the resonator electrodes and thus confines much better the longitudinal acoustic standing waves inside the resonator.