gebhart/patrepeval huggingface dataset:
Piezoelectric thin films and their applications for electronics (2009)

ZnO and AlN piezoelectric thin films have been studied for applications in bulk acoustic wave (BAW) resonator. This article introduces methods of forming ZnO and AlN piezoelectric thin films by radio frequency sputtering and applications of BAW resonators considering the relationship between the crystallinity of piezoelectric thin films and the characteristics of the BAW resonators. Using ZnO thin films, BAW resonators were fabricated for a contour mode at 3.58 MHz and thickness modes from 200 MHz to 5 GHz. The ZnO thin films were combined with various materials, substrates, and thin films to minimize the temperature coefficient of frequency (TCF). The minimum TCF of BAW resonators was approximately 2 ppm/°C in the range −20 to 80 °C. The electromechanical coupling coefficient (k2) in a 1.9 GHz BAW resonator was 6.9%. Using AlN thin films, 5–20 GHz BAW resonators with an ultrathin membrane were realized. The membrane thickness of a 20 GHz BAW resonator was about 200 nm, k2 was 6.1%, and the quality factor (Q) was about 280. Q decreased with increasing resonant frequency. The value of k2 is almost the same for 5–20 GHz resonators. This result could be obtained by improving the thickness uniformity, by controlling internal stress of thin films, and by controlling the crystallinity of AlN piezoelectric thin film.
Theory of thermal diffusivity by pulse technique (1966)

General solutions of the heat conduction equation are presented for axially symmetric pulse heating of a cylindrical sample with constant surface conditions. The solutions, established by means of Green's functions, are applicable to cases in which the radial and axial components may be treated separately and the heat source function is of the form Q f(x) g(r) ψ(t). Particular results already presented in the literature are derived, as well as more general formulae. Reference is made to a novel form of digital computing technique for finding the values of parameters which give the best correlation of theoretical and experimental temperature-time characteristics.
Pulsed-plasma polymerization of 1-vinyl-2-pyrrolidone: Synthesis of a linear polymer (1998)

The RF plasma induced polymerization of 1-vinyl-2-pyrrolidone was examined under variable duty-cycle pulsed-plasma conditions. Large-scale progressive changes in the composition of the resultant polymeric films were observed with sequential changes in the plasma duty cycle employed during polymerization, all other plasma variables held constant. The film compositional changes obtained are in the direction of increased retention of the lactam ring of the monomer in the resultant polymers as the duty cycles employed (i.e., the ratio of plasma on to plasma off times) were decreased. Particularly significant are the relatively linear polymeric structures obtained under the exceptionally low-average power deposition conditions made accessible with the pulsed plasma technique. XPS and FTIR spectroscopic examination of these latter films reveal compositions that are similar to those obtained by conventional (i.e., nonplasma) synthesis of the linear polymer. The film chemistry controllability demonstrated in the present study is achieved while maintaining the many advantages of the plasma polymerization approach for surface modifications. This work provides additional support for use of the pulsed operational mode as an effective means of film chemistry control, in particular extending the plasma polymerization technique to include synthesis of linear polymers, in lieu of the more highly crosslinked structures typically produced in conventional continuous-wave plasma polymerization processes. © 1998 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 36: 3121–3129, 1998
Acoustic immunosensor for real-time sensing of neurotransmitter GABA (2004)

An immunosensing method is described for the measurement of /spl gamma/-amino-4-butyric acid (GABA), one of two inhibitory neurotransmitters in the central nervous system. Monoclonal antibody for GABA was immobilized on the gold electrodes of quartz crystal. Binding of GABA produced a decrease in the resonant frequency of quartz crystal. In in-vitro experiments, the sensor demonstrated a sensitivity of 600Hz decrease in resonant frequency in response to 49 mM change in GABA concentration. The response time was less than 10 seconds. The binding sites of immobilized antibody were successfully regenerated by repeated washing with ImM HCl solution. A significant increase in the resistance of the electrical model of the quartz crystal indicated an increase in the viscosity due to the binding of GABA to the anti-body. An acoustic immunosensor sensitive and specific to GABA was demonstrated. Real-time sensing of GABA will enable greater insights into several neuropathologies like epilepsy, Parkinson's disease etc. where the role of GABA is critical.
Synthesis of aluminum oxide nanoparticles in an aqueous ammonium-formaldehyde solution (2006)

A method of synthesis of the organic-inorganic nanocomposite consisting of a paraformaldehyde matrix and aluminum oxide nanoparticles is developed. Spontaneous dispersion of the composite in water at various component ratios makes it possible to prepare a sol or gel of hydrated aluminum oxide. No changes in the oxide particle dimensions are observed during storage of the composite.
Homologous Expression of Recombinant Lignin Peroxidase in <i>Phanerochaete chrysosporium</i> (1999)

The promoter region of the glyceraldehyde-3-phosphate dehydrogenase gene (gpd) was used to drive expression of mnp1, the gene encoding Mn peroxidase isozyme 1, in primary metabolic cultures of Phanerochaete chrysosporium. A 1,100-bp fragment of the P. chrysosporium gpd promoter region was fused upstream of the mnp1 gene to construct plasmid pAGM1, which contained the Schizophyllum commune ade5 gene as a selectable marker. pAGM1 was used to transform a P. chrysosporium ade1 auxotroph to prototrophy. Ade+ transformants were screened for peroxidase activity on a solid medium containing high carbon and high nitrogen (2% glucose and 24 mM NH4 tartrate) and o-anisidine as the peroxidase substrate. Several transformants that expressed high peroxidase activities were purified and analyzed further in liquid cultures. Recombinant Mn peroxidase (rMnP) was expressed and secreted by transformant cultures on day 2 under primary metabolic growth conditions (high carbon and high nitrogen), whereas endogenous wild-type mnp genes were not expressed under these conditions. Expression of rMnP was not influenced by the level of Mn in the culture medium, as previously observed for the wild-type Mn peroxidase (wtMnP). The amount of active rMnP expressed and secreted in this system was comparable to the amount of enzyme expressed by the wild-type strain under ligninolytic conditions. rMnP was purified to homogeneity by using DEAE-Sepharose chromatography, Blue Agarose chromatography, and Mono Q column chromatography. The M(r) and absorption spectrum of rMnP were essentially identical to the M(r) and absorption spectrum of wtMnP, indicating that heme insertion, folding, and secretion were normal.(ABSTRACT TRUNCATED AT 250 WORDS)
Antimicrobial Activity of Isopteropodine (2005)

Bioassay-directed fractionation for the determination of antimicrobial activity of Uncaria tomentosa, has led to the isolation of isopteropodine (0.3%), a known Uncaria pentacyclic oxindol alkaloid that exhibited antibacterial activity against Gram positive bacteria.
Growth Hormone Receptor Deficiency Is Associated with a Major Reduction in Pro-Aging Signaling, Cancer, and Diabetes in Humans (2011)

Mutations in growth signaling pathways extend life span, as well as protect against age-dependent DNA damage in yeast and decrease insulin resistance and cancer in mice. To test their effect in humans, we monitored for 22 years Ecuadorian individuals who carry mutations in the growth hormone receptor (GHR) gene that lead to severe GHR and IGF-1 (insulin-like growth factor-1) deficiencies. We combined this information with surveys to identify the cause and age of death for individuals in this community who died before this period. The individuals with GHR deficiency exhibited only one nonlethal malignancy and no cases of diabetes, in contrast to a prevalence of 17% for cancer and 5% for diabetes in control subjects. A possible explanation for the very low incidence of cancer was suggested by in vitro studies: Serum from subjects with GHR deficiency reduced DNA breaks but increased apoptosis in human mammary epithelial cells treated with hydrogen peroxide. Serum from GHR-deficient subjects also caused reduced expression of RAS, PKA (protein kinase A), and TOR (target of rapamycin) and up-regulation of SOD2 (superoxide dismutase 2) in treated cells, changes that promote cellular protection and life-span extension in model organisms. We also observed reduced insulin concentrations (1.4 μU/ml versus 4.4 μU/ml in unaffected relatives) and a very low HOMA-IR (homeostatic model assessment-insulin resistance) index (0.34 versus 0.96 in unaffected relatives) in individuals with GHR deficiency, indicating higher insulin sensitivity, which could explain the absence of diabetes in these subjects. These results provide evidence for a role of evolutionarily conserved pathways in the control of aging and disease burden in humans.
Advanced cooling techniques for high-pressure, hydrocarbon-fueled rocket engines (1980)

The regenerative cooling limits (maximum chamber pressure) for 02/hydrocarbon gas generator and staged combustion cycle rocket engines over a thrust range of 89,000 N (20,000lbf) to 2,669,000 N (600,000 lbf) for a reusable life of 250 missions were defined. Maximum chamber pressure limits were first determined for the three propellant combinations (O2/CH4, O2/C3H8, and O2/RP-1 without a carbon layer (unenhanced designs). Chamber pressure cooling enhancement limits were then established for seven thermal barriers. The thermal barriers evaluated for these designs were: carbon layer, ceramic coating, graphite liner, film cooling, transpiration cooling, zoned combustion, and a combination of two of the above. All fluid barriers were assessed a 3 percent performance loss. Sensitivity studies were then conducted to determine the influence of cycle life and RP-1 decomposition temperature on chamber pressure limits. Chamber and nozzle design parameters are presented for the unenahanced and enhanced designs. The maximum regenerative cooled chamber pressure limits were attained with the O2/CH4 propellant combination. The O2/RP-1 designs relied on a carbon layer and liquid gas injection chamber contours, short chamber, to be competitive with the other two propellant combinations. This was attributed to the low decomposition temperature of RP-1.
Significance of β-Galactoside α2,6 Sialyltranferase 1 in Cancers (2015)

Altered glycosylation is a common feature of cancer cells. It takes a variety of forms, which includes loss of expression or excessive expression of some structures, the accumulation of precursors, the appearance of novel structures, etc. Notably, these changes in glycan structure do not occur as a random consequence of disorder biology. Only a limited subset of oligosaccharides is found frequently enriched on the tumor cell surface and implicated in different tumor phenotypes. Among these, altered sialylation has long been associated with metastatic cell behaviors such as invasion and enhanced cell survival and accumulating evidence points to the alteration occurring in the sialic acid linkage to other sugars, which normally exists in three main configurations: α2,3, α2,6, and α2,8, catalyzed by a group of sialyltransferases. The aberrant expression of all three configurations has been described in cancer progression. However, the increased α2,6 sialylation catalyzed by β-galactoside α2,6 sialyltranferase 1 (ST6Gal I) is frequently observed in many types of the cancers. In this review, we describe the findings on the role of ST6Gal I in cancer progression, and highlight in particular the knowledge of how ST6Gal I-mediated α2,6 sialylated glycans or sialylated carrier proteins regulate cell signaling to promote the malignant phenotype of human carcinoma.
CFTR mRNA expression is regulated by an upstream open reading frame and RNA secondary structure in its 5′ untranslated region (2014)

Post-transcriptional regulation of gene expression through 5' untranslated region (5'UTR)-encoded cis-acting elements is an important mechanism for the control of protein expression levels. Through controlling specific aspects of translation initiation, expression can be tightly regulated while remaining responsive to cellular requirements. With respect to cystic fibrosis (CF), the overexpression of cystic fibrosis transmembrane conductance regulator (CFTR) protein trafficking mutants, such as delta-F508, is of great biological and clinical interest. By understanding the post-transcriptional mechanisms that regulate CFTR expression, new procedures can be developed to enhance CFTR expression in homozygous delta-F508 CF patients. We have identified the key elements of a complex negative regulatory mechanism that is encoded within the human CFTR 5'UTR and show how these elements act in combination to restrict CFTR gene expression to a consistently low level in a transcript-specific manner. This study shows, for the first time, that endogenous human CFTR expression is post-transcriptionally regulated through a 5'UTR-mediated mechanism. We show that the very low levels of endogenous CFTR expression, compared with other low expression genes, are maintained through the co-operative inhibitory effects of an upstream open reading frame and a thermodynamically stable RNA secondary structure.
OLAP and Statistical Databases: Similarities and Differences. (1996)

During the 1980&apos;s there was a lot of activity in the area of Statistical Databases, focusing mostly on socio-economic type applications, such as census data, national production and consumption patterns, etc. In the 1990&apos;s the area of On-Line-Analytic Processing (OLAP) was introduced for the analysis of transaction based business data, such as retail stores transactions. Both areas deal with the representation and support of data in a multi-dimensional space. Much of the OLAP literature does not refer to the Statistical Database literature, perhaps because the connection between analyzing business data and socio-economic data is not obvious. Furthermore, there are papers published in one area or the other whose results can be applied in both application areas. In this paper, we compare the work done in these two areas. We discuss concepts used in the conceptual modeling of the data and operations over them, efficient physical organization and access methods, as well as privaccy issues. We point out the terminology used and the correspondence between terms. We identify which research aspects are emphasized in each of these areas and the reasons for that. We conclude by arguing for the support of a Statistical Object data type as one of the fundamental structures that object-oriented data models and systems should support.
A multiaccess protocol for high-speed WLAN (2002)

There is growing interest in very high speed local area networks capable of transporting ATM and multimedia traffic, and very wide bands (100s MHz) are becoming available to that effect. This paper introduces and analyzes some characteristics of a protocol to access and utilize all the available bandwidth without putting undue strain on the capabilities of individual transceivers.
Debatable Premises in Telecom Policy, 31 J. Marshall J. Info. Tech. & Privacy L. 453 (2015) (2015)

The five premises that this paper considers are: 1. Everyone needs low-cost access to high speed broadband service 2. High-speed broadband is necessary for education, health, government, and other social services 3. Wireless can‟t compete with cable 4. An open Internet is necessary for innovation and necessarily benefits consumers 5. Telecommunications are better somewhere else.
Direct Gene Transfer for the Understanding and Treatment of Human Disease (1994)

Direct gene transfer has been used to develop molecular genetic interventions for acquired diseases in several animal models. Through the use of intravascular catheters or anatomically localized injection of DNA liposome complexes, specific tissues can be transduced with recombinant genes. Several promising applications of this method for the study of vascular biology have been demonstrated by direct gene transfer into arteries in vivo. Delivery, via catheter, of genes that modulate the thrombogenic or proliferative properties of vascular cells may someday provide therapy for stenotic lesions of atherosclerosis or following angioplasty. Cancer is another acquired disorder in which direct gene transfer may improve the efficacy of treatment. Introduction of class I MHC or cytokine genes with antitumor or immunostimulatory effects have demonstrated promise in animal models. Direct transfer of an allogeneic class I MHC gene into tumors in vivo induces a CD8+ CTL response against weak antigens on poorly immunogenic tumors. The efficacy of this antitumor response can be augmented to induce regression of actively growing established tumors. Additional strategies, such as intratumoral delivery of combinations of multiple cytokine and MHC genes, may serve to improve the antitumor response. A clinical gene therapy protocol is underway to analyze the safety and efficacy of DNA liposome-mediated gene transfer in humans. Development of improved gene delivery systems and introduction of recombinant genes into visceral tumors by intravascular catheter will extend the application of direct gene transfer to immunotherapy of malignancies. These clinical trials of direct gene transfer will help to develop new treatment strategies for human diseases.
Coupling of a mutated form of the human beta 2-adrenergic receptor to Gi and Gs. Requirement for multiple cytoplasmic domains in the coupling process. (1991)

We constructed five genes encoding mutant human beta 2-adrenergic receptor sequence (beta 2AR) which contained 12-22 amino acid substitutions with corresponding sequence from the human alpha 2AAR in order to assess the receptor domains involved in Gs versus Gi recognition and coupling. Mutant beta 2AR with substitutions in the N (S1)- and C-terminal (S2) portions of the third intracellular loop, the proximal cytoplasmic tail (S3), and two combinations thereof (S2,3 and S1,2,3), were stably expressed in Chinese hamster fibrobasts (CHW-1102), as were the human beta 2AR and alpha 2AAR at comparable receptor levels. All mutant receptors with S2 substitutions (i.e. S2, S2,3, S1,2,3) were significantly (approximately 85%) uncoupled from Gs. Upon exposure to pertussis toxin, which uncouples receptors from Gi, S1,2,3 exhibited a 526 +/- 99% increase in agonist-stimulated adenylylcyclase activity compared with a 59 +/- 13% increase with the wild type receptor. This enhanced ability of S1,2,3 to interact with Gs following pertussis toxin treatment indicates that, in the absence of toxin exposure, substantial coupling occurs between the mutant receptor and Gi. Mutant beta 2AR bearing only one or two alpha 2AAR-substituted sequences showed no such enhancement. Forskolin-stimulated enzyme activities were increased by pertussis toxin treatment to similar degrees in all clones examined, indicating that the observed effects are confined to the receptor-mediated pathway. In the absence of GTP, competition binding experiments with S1,2,3, beta 2AR and alpha 2AAR revealed that approximately 40-50% of the receptors formed a high affinity binding state for agonist. Pertussis toxin treatment markedly reduced this to approximately 19% with S1,2,3, while having no effect on beta 2AR and completely eliminating high affinity agonist binding to alpha 2AAR. These results suggest that S1,2,3 interacts with Gi as well as Gs, and that receptor:G protein coupling requires the concerted participation of multiple cytoplasmic receptor domains.
High-level library mapping for arithmetic components (1996)

We describe high-level library mapping (HLLM), a technique that permits reuse of complex RT-level databook components (specifically ALUs). HLLM can be used to couple existing databook libraries, module generators and custom-designed components with the output of architectural or behavioral synthesis. In this paper, we define the problem of high-level library mapping, present some algorithmic formulations for HLLM of ALUs, and demonstrate the versatility of our approach on a variety of libraries. We also compare HLLM against the traditional mapping approach using logic synthesis. Our experiments show that HLLM for ALUs outperforms logic synthesis in area, delay, and runtime, indicating that HLLM is a promising approach for reuse of datapath components in architectural design and high-level synthesis.
L-Asparaginase Production by Various Bacteria (1969)

Of 123 species of bacteria surveyed for L-asparaginase synthesis, Erwinia aroideae NRRL B-138 provided the highest yields.
Effects of incremental amounts of extruded linseed on the milk fatty acid composition of dairy cows receiving hay or corn silage (2013)

The effect of supplementation of increasing amounts of extruded linseed in diets based on hay (H; experiment 1) or corn silage (CS; experiment 2) was investigated in regard to dairy performance and the milk fatty acid (FA) composition. In each experiment, 4 lactating multiparous Holstein cows were used in a 4 × 4 Latin square design (28-d periods). The cows were fed a diet (50:50 and 40:60 concentrate:forage ratio for experiments 1 and 2, respectively; dry matter basis) without supplementation (H0 or CS0) or supplemented with 5% (H5 or CS5), 10% (H10 or CS10), or 15% (H15 or CS15) of extruded linseed. Regardless of the forage type, diet supplementation with increasing amounts of extruded linseed had no effect on the dry matter intake, milk yield, or protein content or yield. In contrast, the milk fat content decreased progressively from H0 to H10 diets, and then decreased strongly with the H15 diet in response to increasing amounts of extruded linseed. For CS diets, the milk fat content initially decreased from CS0 to CS10, but then increased with the CS15 diet. For the H diets, the milk saturated FA decreased (-24.1g/100g of FA) linearly with increasing amounts of extruded linseed, whereas the milk monounsaturated FA (+19.0 g/100 g), polyunsaturated FA (+4.9 g/100 g), and total trans FA (+14.7 g/100 g) increased linearly. For the CS diets, the extent of the changes in the milk FA composition was generally lower than for the H diets. Milk 12:0 to 16:0 decreased in a similar manner in the 2 experiments with increasing amounts of extruded linseed intake, whereas 18:0 and cis-9 18:1 increased. The response of total trans 18:1 was slightly higher for the CS than H diets. The milk trans-10 18:1 content increased more with the CS than the H diets. The milk cis-9,trans-11 conjugated linoleic acid response to increasing amounts of extruded linseed intake was linear and curvilinear for the H diets, whereas it was only linear for the CS diets. The milk 18:3n-3 percentage increased in a similar logarithmic manner in the 2 experiments. It was concluded that the milk FA composition can be altered by extruded linseed supplementation with increasing concentrations of potentially health-beneficial FA (i.e., oleic acid, 18:3n-3, cis-9,trans-11 conjugated linoleic acid, and odd- and branched-chain FA) and decreasing concentrations of saturated FA. Extruded linseed supplementation increased the milk trans FA percentage.