gebhart/patrepeval huggingface dataset:
Isolation and Culture of Epithelial Stem Cells (2008)

In the skin, epithelial stem cells in the hair follicle contribute not only to the generation of a new hair follicle with each hair cycle, but also to the repair of the epidermis during wound healing. When these stem cells are isolated and expanded in culture, they can give rise to hair follicles, sebaceous glands, and epidermis when combined with dermis and grafted back onto Nude mice. In this chapter, we provide a method for isolating hair follicle epithelial stem cells from the skin of adult mice using immunofluorescent labeling to allow for the specific purification of epithelial stem cells by fluorescence-activated cell sorting (FACS). Notably, this method relies exclusively on cell surface markers, making it suitable for use with any strain of mouse and at various stages of the hair cycle. We also provide a detailed protocol for culturing epithelial stem cells isolated by FACS, allowing for analysis using a wide variety of culture assays. Additionally, we provide notes on using cultured cells for specific applications, such as viral manipulation and grafting. These techniques should be useful for directly evaluating stem cell function in normal mice and in mice with skin defects.
Blockchain Support for Flexible Queries with Granular Access Control to Electronic Medical Records (EMR) (2018)

In this paper, we propose an architecture for Blockchain-based Electronic Medical Records (EMRs) called GAA-FQ (Granular Access Authorisation supporting Flexible Queries) that comprises an access model and an access authorisation scheme. Unlike existing Blockchain schemes, our access model can authorise different levels of granularity of authorisation, whilst maintaining compatibility with the underlying Blockchain data structure. Furthermore, the authorisation, encryption, and decryption algorithms proposed in the GAA-FQ scheme dispense with the need to use a public key infrastructure (PKI) and hence improve the computation performance needed to support more granular and distributed, yet authorised, EMR data queries. We validated the computation performance and transmission efficiency for GAA-FQ using a simulation of GAA-FQ against an access control scheme for EMRs called ESPAC as our baseline that was not designed using a Blockchain. To the best of our knowledge, GAA- FQ is the first Blockchain-oriented access authorisation scheme with granular access control, supporting flexible data queries, that has been proposed for secure EMR information management.
De la energía de las olas al balance y de este a los sistemas de a bordo (2017)

espanolCon el objetivo de reducir el consumo de los buques y, por tanto, los costes medioambientales y economicos asociados al mismo, este articulo analizara el aprovechamiento de energia contenida en la mar de forma util, sencilla y directa para ser consumida por los sistemas de a bordo, mejorando asi la eficiencia total del navio. Son ya numerosos los dispositivos marinos disenados para la transformacion de energia undimotriz en energia electrica para su posterior consumo. Pero ?que mejor artefacto marino, flotante y consumidor de energia que un buque? Esta es la motivacion que alimenta este articulo, en el que se va a presentar un sistema innovador para la generacion de energia a bordo, que apunta al balance de un buque como medio de transformacion de la energia undimotriz en energia hidraulica contenida en sus tanques estabilizadores. El sistema contara con una turbina hidraulica, una serie de valvulas automaticas y un sistema de control de las mismas que mejorara la eficiencia de la turbina y asegurara el correcto comportamiento dinamico del buque en la mar. EnglishIn order to reduce the overall fuel consumption of the ship and. Of course, the environmental and economic cost associated to it, this article will evaluate the exploitation of the energy available at the ocean in a direct, useful and simple way in order to be consumed later on by the systems onboard, improving the general efficiency of the ship. Nowadays, there are many offshore devices able to transform energy from the waves into electricity to be consumed. Is there any more suitable marine system, floating and main energy consumer than a vessel? This is the motivation of this article, which points out that the ship’s roll motion could be used to take advantage of the available energy at sea, as when the ship rolls hydraulic energy is generated in the “anti-rolling” tank of the ship. The system will include a hydraulic turbine, some automatic valves and the control system of these valves, whose purpose is to improve the efficiency of the turbine and, even more important, to ensure a proper seakeeping behavior.
Transient global amnesia: a review emphasizing pathogenic aspects (2000)

The transitory memory disturbance known as transient global amnesia (TGA) remains an enigma from a pathogenic point of view. In spite of its typical benign prognosis, TGA is a frightening experience for patients and their relatives. Moreover, a TGA episode usually leads to extensive investigation of patients in search of organic alterations that might be responsible for the event. Finally, TGA generates queries about therapeutic choices. In this review, we critically re-evaluate the evidence in support of and against the three main pathogenic hypotheses (i.e. ischemia, seizure discharge, and migraine), and we conclude that none of these appears completely convincing. Given the good prognosis and the lack of association with organic and instrumental abnormalities, we advance the hypothesis that TGA may be related to psychological disturbances causing transient alteration in brain metabolism and, consequently, amnesia. Our conclusion has relevant consequences in the evaluation of patients with TGA.
대학생의 진로 불안감 변화에 따른 잠재계층 분류 및 영향요인 검증 (2011)

본 연구의 목적은 대학생의 진로 불안감 변화에 따라 구분되는 잠재계층(집단)의 수와 그 변화 형태를 알아보고, 각 잠재계층의 분류에 영향력을 미치는지 요인들을 검증하는 것이다. 한국교육고용패널조사의 고등학교 3학년 코호트 자료에 Nagin(1999)의 준모수적 집단 중심 접근 방법(semi-parametric group-based approach)을 적용한 결과, 3개의 잠재계층이 도출되었고, 변화 형태에 따라 저수준 집단, 증가 집단, 고수준 집단으로 명명하였다. 다항로짓분석을 통해 영향요인을 검증한 결과 성별, 특기인지, 결단력, 계획실천능력, 학과만족도, 대학성적이 잠재계층을 결정하는 데 유의미한 변수로 나타났다. 본 연구는 진로 불안감 변화에 영향을 줄 수 있는 변수들을 바탕으로 잠재계층을 예측하여 진로에 대한 불안감을 높은 상태로 유지하는 학생들을 위한 적절한 대비책을 찾는 데 도움을 줄 것이다.
A highly damage-resistant zn : linbo/sub 3/ ridge waveguide and its application to a polarization-independent wavelength converter (2003)

We have fabricated a periodically poled ridge waveguide using a liquid phase epitaxy (LPE)-grown Zn:LiNbO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> substrate. The waveguide can confine both the transverse electric and transverse magnetic mode. Its strong resistance to photorefractive damage enables stable parametric amplification. We demonstrate polarization-independent wavelength conversion using polarization diversity in a single waveguide.
Endocrinological study of patients with meningitis tuberculosa. (1981)

We recorded two cases of endocrine disturbance following tuberculous meningitis associated with intracranial calcification in the suprasellar region. Both patients showed hypogonadism, delayed bone-maturation, obesity, polydipsia and polyuria. A giant gynecomastia was seen in one of them. In discussion we considered the relation of gynecomastia and endocrinological disturbance following hypopituitarism. Prolonged administration of clomiphene citrate and synthetic LH-RHwas not effective in promoting sexual maturity in these patients.
Measurement of picomole amounts of any inositol phosphate isomer separable by h.p.l.c. by means of a bioluminescence assay (1991)

An assay is described which allows the determination of the mass of any individual inositol phosphate (InsP) isomer by combining a popular h.p.l.c. separation method with simple desalting, dephosphorylation and final measurement of Ins liberated using an inositol dehydrogenase-NADH-linked bioluminescence reaction. The limit of sensitivity of this assay is about 1 pmol of Ins. routinely 5 pmol. About 40 mg wet wt. of guinea pig small intestine longitudinal smooth muscle contains 5 pmol of Ins(1,4,5)P3. For Inst(1,3,4)P3 or Ins(1,3,4,5)P4 slightly more smooth muscle is needed, and for major isomers of InsP1 or InsP2 10 mg wet wt. or less of tissue can be used. A 35 mm tissue culture plate with a confluent layer of rat fibroblasts contains about 30 pmol of Ins(1,4,5)P3. The method was applied to the measurement of the masses of Ins1P1. Ins4P1, Ins(1,4)P2, Ins(1,3,4)P3, Ins(1,4,5)P3, Ins(1,3,4,5)P4 and InsP5. The h.p.l.c. elution profiles of radiolabelled InsPS generated from [32P]Pi-labelled human Erythrocytes, [3H]Ins-labelled cultured rat fibroblasts and [3H]Ins-labelled smooth muscle fragments from guinea pig small intestine were compared with the h.p.l.c. elution profiles of their masses.
Engineering the performance of mixed matrix membranes for gas separations (2007)

Mixed matrix membranes that comprise domains of organic and inorganic components are investigated in this research. Such materials effectively circumvent the polymeric 'upper bound trade-off curve' and show properties highly attractive for industrial gas separations. Nevertheless, lack of intrinsic compatibility between the organic polymers and inorganic fillers poses the biggest challenge to successful fabrication of mixed matrix membranes. Consequently, control of the nanoscale interface between the sieve and polymer has been the key technical challenge to the implementation of composite membrane materials. The overarching goal of this research was to devise and explore approaches to enhance the performance of mixed matrix membranes by properly tailoring the sieve/polymer interface. \n In an effort to pursue the aforementioned objective, three approaches were developed and inspected: (i) use of silane coupling agents, (ii) hydrophobizing of sieve surface through alcohol etherification reactions, and (iii) a two-step modification sequence involving the use of a Grignard reagent. A comparison was drawn to evaluate these methodologies and the most effective strategy (Grignard treatment) was selected and further investigated.\n Successful formulation and characterization of mixed matrix membranes constituting zeolite 4A modified via the Grignard treatment are described in detail. Membranes with impressive improvements in gas separation efficiency and mechanical properties were demonstrated. The basis for the improvements in polymer/sieve compatibility enabled by this specific process were proposed and investigated.\n A key aspect of the present study was illuminating the detailed chemical mechanisms involved in the Grignard modification. Systematic characterization and carefully designed experiments revealed that the formation of distinctive surface structures is essentially a heterogeneous nucleation process, where Mg(OH)2 crystals grow from the nuclei previously extracted from zeolites.\n In addition to the main work, discovery of sonication-induced dealumination of zeolites was made during the systematic exploration of Grignard chemistry. The new procedure employing sonication can potentially be applied to prepare zeolites with a variety of Si/Al ratios under relatively mild conditions.\n The last part of this thesis focused on development of a technique to generalize the highly specific Grignard treatment to inorganic materials other than zeolite 4A. This work delivered composite membranes with improved interfacial adhesion. Moreover, research revealed the effect of surface nuclei density on the ultimate morphology of deposited nanostructures and how different surface morphologies influence polymer/filler interaction in composite membranes. Methods were devised to tailor the morphologies of such structures in order to optimize adhesion enhancement. The acquired results demonstrated the potential of extending this modification process to a broad domain of materials and render it a general methodology for interfacial adhesion promotion.
La Edad Biológica en la Preparación Física de las Atletas de Baloncesto Categoría 11-12 años de la EIDE Ormani Arenado de Pinar del Río (2009)

Este trabajo es el resultado de un largo y minucioso proceso investigativo encaminado al estudio del proceso del entrenamiento y su relación con la edad biológica de las atletas escolares de baloncesto femenino, para perfeccionar el proceso de preparación física del deportista y contar con un criterio científico y preciso para el entrenamiento deportivo en las edades tempranas. Se apoya en un conjunto de métodos científicos teóricos y empíricos, dentro de los teóricos el Análisis-Síntesis, Inductivo-Deductivo, Histórico-Lógico y entre los empíricos: la entrevista y la medición, todo ello encaminado al diagnóstico, la sistematización de los conceptos más actualizados a que tuvimos acceso sobre el tema y en los resultados finales que derivaron en la propuesta de un conjunto de acciones. Se empleó una muestra de 25 atletas, 5 entrenadores de Baloncesto, 2 Médicos Deportivos y 3 Directivos de las comisiones provinciales a los cuales se le aplicaron los diferentes métodos empleados. Los resultados se procesaron estadísticamente por el método matemático de análisis porcentual y se determinaron estadígrafos tales como la media y la desviación Standard. Como resultado se proponen un conjunto de acciones que permiten la incorporación del criterio de la edad biológica en la preparación física de las atletas de baloncesto de la categoría 11-12 años femenino.
Systemic Treatment for Chlorotic Trees (1980)

Abstract Ferric citrate injected in powdered form was effective in correcting iron chlorosis of pin oak, cypress, red oak, sweet gum, and swamp white oak. Chlorotic white oak and American sycamore did not respond significantly to systemic iron treatments. Both ferric ammonium citrate and ferric citrate were effective in correcting moderate to advanced stages of chlorosis in large pin oak and sweet gum. An inexpensive method of injecting premeasured chemical powders is reported.
Compression degradation metrics for analysis of consistency in microcalcification detection (2002)

The authors motivate, define and illustrate the behavior of four scalar metrics which measure the degree to which microcalcification (/spl mu/uCa++) detection is affected by lossy compression distortion. The JPEG method is used at various compression rates determined by a single parameter. The authors' metrics are specifically targeted to measure the degree of invariance of the processing to the presence of a compression-decompression step and not absolute processing success. The detection of the /spl mu/Ca++s is done with a two-step process of selective median filtering and wavelet domain hard thresholding with saturation. This latter step produces a binary image revealing small structures which are the desired objects in the image. The authors have also chosen to perform the enhancement before the compression step as suggested by their previous work to allow higher compression rates. In the system used to do the comparison, the two branches of processing are identical except for the compression/decompression step and their results are binary images. The inputs to the metric computation system are these two images which produce four scalar metrics which are various percentages of differences in pixel and region activity. Using seven image sections from mammograms, the authors illustrate the results at various compression ratios in the range 28:1 to 42:1 for each image and also averaged over the set of seven. The three pixel based metrics decrease monotonically as the JPEG compression quality parameter increases to reduce the compression ratio. The region based metric does not change monotonically due to the more complex nature of region analysis.
Design and performance characteristics of a whole-body positron transaxial tomograph. (1976)

A whole-body positron-emission transaxial tomograph (PETT III) is described in detail and evaluated in terms of resolution, accuracy, and efficiency. The PETT III utilizes annihilation coincidence detection to provide spatial resolution; high sensitivity is achieved by using 48 Nal(tl) detectors set in a hexagonal array with a multiple-coincidence logic. The assumptions and approximations made in the reconstruction and their effect on image quality are discussed. Phantom studies shows the depth-independent resolution and response of PETT III, as well as its ability to recover activity distribution quantitatively in the cross section measured. Images obtained with patients and normal volunteers show the potential clinical utility of PETT III.
Impact of anti-apoptotic and anti-oxidative effects of bone marrow mesenchymal stem cells with transient overexpression of heme oxygenase-1 on myocardial ischemia (2010)

Although mesenchymal stem cells (MSCs) have therapeutic potential for tissue injury, intolerance and poor cell viability limit their reparative capability. Therefore, we examined the impact of bone marrow-derived MSCs, in which heme oxygenase-1 (HO-1) was transiently overexpressed, on the repair of an ischemic myocardial injury. When MSCs and HO-1-overexpressed MSCs (MSC(HO-1)) were exposed to serum deprivation/hypoxia or H(2)O(2)-induced oxidative stress, MSC(HO-1) exhibited increased resistance to cell apoptosis compared with MSCs (17 +/- 1 vs. 30 +/- 2%, P < 0.05) and were markedly resistant to cell death (2 +/- 1 vs. 32 +/- 2%, P < 0.05). Under these conditions, vascular endothelial growth factor (VEGF) production was 2.1-fold greater in MSC(HO-1) than in MSCs. Pretreatment of MSCs and MSC(HO-1) with phosphatidylinositol 3-kinase (PI 3-kinase)/protein kinase B (Akt) pathway inhibitors such as LY-294002 (50 muM) or wortmannin (100 nM) significantly decreased VEGF production. In a rat infarction model with MSCs or MSC(HO-1) (5 x 10(6) +/- 0.1 x 10(6) cells/rat) transplantation, the number of TdT-mediated dUTP nick end-labeling-positive cells was significantly lower in the MSC(HO-1) group than in the MSC group (12.1 +/- 1.0 cells/field vs. 26.5 +/- 2.6, P < 0.05) on the 4th day after cell transplantation. On the 28th day, increased capillary density associated with decreased infarction size was observed in the MSC(HO-1) group (1,415 +/- 47/mm(2) with 21.6 +/- 2.3%) compared with those in the MSCs group (1,215 +/- 43/mm(2) with 28.2 +/- 2.3%, P < 0.05), although infarction size relative to area at risk was not different in each group at 24 h after transplantation. These results demonstrate that MSC(HO-1) exhibit markedly enhanced anti-apoptotic and anti-oxidative capabilities compared with MSCs, thus contributing to improved repair of ischemic myocardial injury through cell survival and VEGF production associated with the PI 3-kinase/Akt pathway.
A unified approach to time- and frequency-domain realization of FIR adaptive digital filters (1983)

Specific implementations of the finite impulse response (FIR) block adaptive filter in the frequency domain are presented and some of their important properties are discussed. The time-domain block adaptive filter implemented in the frequency domain is shown to be equivalent to the frequency-domain adaptive filter (derived in the frequency domain), provided data sectioning is done properly. All of the known time- and frequency-domain adaptive filters [1]-[12], [16]-[18] are contained in the set of possible block adaptive filter structures. Thus, the block adaptive filter is generic and its formulation unifies the current theory of time- and frequency-domain FIR adaptive filter structures. A detailed analysis of overlap-save and overlap-add implementations shows that the former is to be preferred for adaptive applications because it requires less computation, a fact that is not true for fixed coefficient filters.
ChemInform Abstract: CLAISEN REARRANGEMENT OF 7‐PRENYL ETHERS OF CHRYSIN AND 3‐O‐METHYLGALANGIN, A NOVEL 3‐O‐DEMETHYLATION (1973)

Abstract Das aus Prenylbromid und Chrysin gebildete Prenylchrysin (Ia) unterwirft sich in siedendem Chinolin einer Claisen‐Umlagerung unter Bildung der säulenchromatographisch getrennten Trimethyldihydrofuro‐Derivate (IIa) und (IIIa).
Turnover of Axonally Transported Phospholipids in Nerve Endings of Retinal Ganglion Cells (1981)

We have investigated the metabolic turnover of axonally transported phospholipids in myelinated axons (optic tract) and nerve endings (superior colliculus) of retinal ganglion cells. One week following intraocular injection of [2-3H]glycerol, turnover rates for individual phospholipid classes in the retina (which contains a number of other cell types in addition to the ganglion cells) were all very similar to each other, with apparent half-lives of approximately 7 days. Apparent half-lives of labeled phospholipids in superior colliculus (presumably primarily in retinal ganglion cell nerve endings) were 10 days for both choline and inositol phosphoglycerides and 13 days for both serine and diacylethanolamine phosphoglycerides. Subcellular fractionation data obtained from superior colliculus at various times after injection suggested that apparent turnover rates determined for nerve ending phospholipids probably were not significantly affected by transfer of axonally transported 3H lipids into myelin. Apparent half-lives for phospholipids in optic tract were somewhat longer than in superior colliculus, ranging from 11 to 18 days. The slower turnover rates in optic tract may, in part, reflect the transfer of some axonal lipids to the more metabolically stable pool of lipids in the myelin ensheathing the retinal ganglion cell axons. In both optic tract and superior colliculus, apparent half-lives for axonally transported phospholipids labeled with [32P]phosphate were only slightly longer than for [2-3H]glycerol, while those for [14C]choline and [3H]acetate were markedly longer, indicating differing degrees of metabolic conservation or reutilization of these precursors relative to glycerol.
6Âª. REUNIÃN NACIONAL DE COMITÃS ESTATALES DE BIOÃTICA (2011)

Durante los dias 6 y 7 de Septiembre del ano en curso, se llevo a cabo la 6a. Reunion Nacional de Comites Estatales de Bioetica organizado por la Comision Nacional de Bioetica que preside el Dr. Manuel H. Ruiz de Chavez, el lema central de la reunion fue “Bioetica y Sociedad”. Tematica de gran trascendencia, ya que ambos conceptos encierran contenidos en continua transformacion, evolucion y perfeccionamiento; producto de los grandes cambios morales, sociales y culturales emanados de las revoluciones cientifica y tecnologica, lo que hace imperioso apremiar una verdadera correspondencia entre ambos aspectos privilegiando en todo momento el bienestar de la sociedad
Mastering Gephi Network Visualization (2015)

Mastering Gephi Network Visualization will take you through an overview of Gephi and network behavior, followed by detailed chapters addressing layouts, filtering, graph statistics, dynamic graphs, and more. You will begin with a concise overview of working with the Gephi interface. You will then see how to create your own graphs and understand the graph layouts to arrange a sample dataset. You will understand the theory behind Dynamic Network Analysis, followed by sample applications on how Gephi can be used to model these networks. You will also learn about the plugins that are most critical for network analysis and graph creation. Finally, you will put together all the previously learned concepts and gain insight on the future state of network graph analysis.After reading this book and following the examples provided, you will have the confidence and expertise to create your own compelling graphs.