gebhart/patrepeval huggingface dataset:
cea-kil operon of the ColE1 plasmid (1983)

We isolated a series of Tn5 transposon insertion mutants and chemically induced mutants with mutations in the region of the ColE1 plasmid that includes the cea (colicin) and imm (immunity) genes. Bacterial cells harboring each of the mutant plasmids were tested for their response to the colicin-inducing agent mitomycin C. All insertion mutations within the cea gene failed to bring about cell killing after mitomycin C treatment. A cea- amber mutation exerted a polar effect on killing by mitomycin C. Two insertions beyond the cea gene but within or near the imm gene also prevented the lethal response to mitomycin C. These findings suggest the presence in the ColE1 plasmid of an operon containing the cea and kil genes whose product is needed for mitomycin C-induced lethality. Bacteria carrying ColE1 plasmids with Tn5 inserted within the cea gene produced serologically cross-reacting fragments of the colicin E1 molecule, the lengths of which were proportional to the distance between the insertion and the promoter end of the cea gene.
Genetic Analysis of the Life Cycle in Interspecific Hybrids of Pleurotus ostreatus and Pleurotus florida Following Protoplast Fusion (1986)

Interspecific hybrids of Pleurotus ostreatus and Pleurotus florida were formed by using protoplasts of complementing auxotrophs. The genetic markers were shown to segregate and recombine in the first generation of monosporus isolates from basidiocarp of seven fusion products. The analysis provides proof of heterokaryosis and strong evidence for haploidy of vegetative nuclei, a sexual cycle consisting of nuclear fusion and meiosis. In all the crosses there was no evidence of linkage between the genetic markers. Clamp connections were formed in monosporus mycelia from basidiocarp of fusion products.
Os sistemas prediais e a promoção da sustentabilidade ambiental (2002)

This paper presents a discussion about the role of building water supply systems for the achievement of environmental sustainability, focussing on the natural water resources. Initially, the importance of environmental sustainability is analysed, and several possible actions regarding the nature and the use of building water supply systems for promoting sustainability are discussed. Considering those actions, some models for managing such actions are proposed, which allow their benefits, risks and costs to be evaluated. At the conclusion, the results of some simulations that illustrate the effects of water conservation actions are discussed. Keywords: environmental sustainability; water conservation; water supply systems; water resources.
Accurate UWB Radar Three-Dimensional Imaging Algorithm for a Complex Boundary Without Range Point Connections (2010)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> Ultrawide-band pulse radars have immeasurable potential for a high-range-resolution imaging in the near field and can be used for noncontact measurement of industrial products with specular or precision surfaces, such as reflector antenna or aircraft fuselage, or identifying and locating the human body in security systems. In our previous work, we developed a stable and high-speed 3-D imaging algorithm, Envelope, which is based on the principle that a target boundary can be expressed as inner or outer envelopes of spheres, which are determined using antenna location and observed ranges. Although Envelope produces a high-resolution image for a simple shape target that may include edges, it requires an exact connection for observed ranges to maintain the imaging quality. For complex shapes or multiple targets, this connection becomes a difficult task because each antenna receives multiple echoes from many scattering points on the target surface. This paper proposes a novel imaging algorithm without range point connection to accomplish high-quality and flexible 3-D imaging for various target shapes. The algorithm uses an accurate estimation for the direction of arrival using signal amplitudes and realizes direct mapping from observed ranges to target points. Several comparative studies of conventional algorithms clarify that our proposed method accomplishes accurate and reliable 3-D imaging even for complex or multiple boundaries. </para>
Cloning of a Gene Whose Expression Is Increased in Scrapie and in Senile Plaques in Human Brain (1985)

A complementary DNA library was constructed from messenger RNA's extracted from the brains of mice infected with the scrapie agent. The library was differentially screened with the objectives of finding clones that might be used as markers of infection and finding clones of genes whose increased expression might be correlated with the pathological changes common to scrapie and Alzheimer's disease. A gene was identified whose expression is increased in scrapie. The complementary DNA corresponding to this gene hybridized preferentially and focally to cells in the brains of scrapie-infected animals. The cloned DNA also hybridized to the neuritic plaques found with increased frequency in brains of patients with Alzheimer's disease.
Tumor Necrosis Factor-α Induces Interleukin-6 Production and Integrin Ligand Expression by Distinct Transduction Pathways (1998)

Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic cytokine that elicits a large number of biological effects. However, the intracellular signaling mechanisms that are responsible for the TNF-alpha effects remain largely unknown. We have previously demonstrated that cultured mouse Sertoli cells, after TNF-alpha treatment, increase the surface expression of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and interleukin-6 (IL-6) production (Riccioli, A., Filippini, A., De Cesaris, P., Barbacci, E., Stefanini, M., Starace, G., and Ziparo, E. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 5808-5812). Here, we show that, in cultured Sertoli cells, TNF-alpha activates the mitogen-activated protein kinase pathway (p38, c-Jun N-terminal protein kinase/stress-activated protein kinase, and the p42/p44 mitogen-activated protein kinases) as revealed by an increased phosphorylation of p38, activating transcription factor-2, c-Jun, and Elk-1. Furthermore, our data indicate that the biological effects induced by TNF-alpha in Sertoli cells (enhancement of ICAM-1, VCAM-1, and IL-6 expression) depend on the activation of different signaling pathways. SB203580, a highly specific p38 inhibitor, does not affect ICAM-1 and VCAM-1 expression, but strongly inhibits IL-6 production. Moreover, interferon-gamma, which up-regulates adhesion molecule expression and reduces IL-6 production, does not induce phosphorylation of p38. Our data strongly support the hypothesis that, in response to TNF-alpha, activation of p38 leads to IL-6 production, whereas ICAM-1 and VCAM-1 expression could be induced by activation of the c-Jun N-terminal protein kinase/stress-activated protein kinase pathway.
Recent progress in the study of anticancer drugs originating from plants and traditional medicines in China. (1994)

Drugs of plant origin have received much attention due to their enormous potential for the prevention and treatment of cancer. Recent progress in the study of anticancer drugs originating from plants and traditional medicines in China is reviewed in this paper, with particular emphasis on taxol, daidzein, acetyl boswellic acid, curcumin and ginsenosid Rh2.
Highly Efficient Visible-Light-Induced Photocatalytic Activity of Fe-Doped TiO2Nanoparticles (2012)

Bare TiO2 and nominal 5.0 at% Fe-doped TiO2 nanoparticles were synthesized by the modified sol-gel method. The samples were physically characterized in order to obtain the correlation between structure and photocatalytic properties by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Brunauer, Emmett and Teller (BET), and UV-vis diffuse reflectance spectrophotometry (UV-vis DRS). XRD results indicated that phase structures of bare TiO2 and Fe-doped TiO2 nanoparticles were the mixture of anatase and rutile phases. The content of rutile phase in 5.0 at% Fe-doped TiO2 nanoparticles decreased. TEM images revealed that the shape of bare and 5.0 at% Fe-doped TiO2 was almost spherical and the average particle size was in the range of 10-30 nm. Specific surface areas of the samples were found as 75 and 134 m 2 /g for bare TiO2 and nominal 5.0 at% Fe-doped TiO2, respectively. The results from UV-vis reflectance spectra clearly indicated the shift of absorption band edge towards visible region upon doping TiO2 with iron. Photocatalytic activity of bare TiO2 and 5.0 at% Fe-doped TiO2 nanoparticles was examined by studying the mineralization of oxalic acid under visible light irradiation and the results clearly showed that Fe-doped TiO2 sample exhibited higher activity than bare TiO2.
The application of inertial and magnetic sensors to the monitoring of calf muscle pump activity (2008)

On long distance journeys passengers at high risk from deep vein thrombosis (DVT) are recommended to exercise on a regular basis to contract the calf muscle pump and encourage venous return. If a passenger fails to complete an exercise program that induces active contraction of the calf muscle pump they will remain at increased risk of DVT. This paper presents a novel inertial and magnetic sensor-based technique for monitoring calf muscle pump activity. The technique could be implemented into a system for monitoring the level of calf muscle pump activity in persons with limited mobility. Such a system could be used to provide a reminder to the user that there is a need to exercise should they have forgotten to exercise, failed to exercise sufficiently or exercised incorrectly. The proposed technique was evaluated by comparison with calf muscle pump activity measured using an electromyography (EMG) sensor. Results show that the technique can be used to monitor calf muscle pump activity over a wide range of leg exercises.
Connectivity analysis of wireless sensor networks with regular topologies in the presence of channel fading (2009)

This paper investigates the probabilistic connectivity of wireless sensor networks in the presence of channel fading. Due to the stochastic nature of wireless channels in sensor networks, the geometric disk shaped model widely used for connectivity analysis of sensor networks can be misleading. In this paper, we develop a mathematical model to evaluate the probabilistic connectivity of sensor networks which incorporates the characteristics of wireless channels, multi-access interference, the network topology and the propagation environment. We present an analytical framework for the computation of node isolation probability and network connectivity under different channel fading models. We also analyze the connectivity of sensor networks in the presence of unreliable sensors. We present numerical and simulation results that compare different regular topologies in terms of several metrics such as node isolation probability, end-to-end connectivity, and network connectivity.
Secure Cloud Storage Based on RLWE Problem (2018)

With the rapid development of cloud storage and quantum computing, ensuring the integrity of the outsourced data for data owners becomes a serious concern. To address this problem, the existing protocols for auditing cloud storage are usually based on the post-quantum cryptographies to check data integrity. Nevertheless, these protocols employ heavy cryptographic operations to construct the data tags, making their efficiency low and extensibility poor. In this paper, we take a new perspective and explore the possibility of designing secure cloud storage (SCS) protocols based on the ring learning with errors problem. Instead of matrix variables, our protocol only utilizes vector variables to generate data block tags so that it has a much lower computational complexity. We then give a strict security proof of cheating resistance against the malicious cloud and privacy guarantee against the curious third-party auditor. We also extend the proposed protocol to support data dynamics and batch auditing for more application scenarios. As a further contribution, we summarize a systematic framework for designing the lattice-based SCS protocols. Finally, exhaustive performance analysis and comparison are provided to verify that the proposed protocol outperforms the existing lattice-based SCS protocols in terms of both operational efficiency and functional extensibility.
Understanding Instrumental Variables in Models with Essential Heterogeneity (2006)

This paper examines the properties of instrumental variables (IV) applied to models with essential heterogeneity, that is, models where responses to interventions are heterogeneous and agents adopt treatments (participate in programs) with at least partial knowledge of their idiosyncratic response. We analyze two-outcome and multiple-outcome models, including ordered and unordered choice models. We allow for transition-specific and general instruments. We generalize previous analyses by developing weights for treatment effects for general instruments. We develop a simple test for the presence of essential heterogeneity. We note the asymmetry of the model of essential heterogeneity: outcomes of choices are heterogeneous in a general way; choices are not. When both choices and outcomes are permitted to be symmetrically heterogeneous, the method of IV breaks down for estimating treatment parameters.
Testing The Shear Strength of Clay in Sweden (1951)

Synopsis In this Paper an account is given of some apparatuses for testing shear strength which have previously been described only in Swedish. The routine methods used in Sweden for testing shear strength are also outlined, and their dependability is discussed. Finally, the reliability of the triaxial test is considered. Dans cette étude on rend compte de quelques appareils pour essayer la résistance au cisaillement qui n'ont été décrits préalablement qu'en suédois. On indique aussi les méthodes de routine útilisées en Suéde pour essayer la résistance au cisaillement, et la croyabilité de ces méthodes est soumise à discussion. Enfin, on examine la véracité de l'essai triaxial.
Dance with Melody (2018)

Dance is greatly influenced by music. Studies on how to synthesize music-oriented dance choreography can promote research in many fields, such as dance teaching and human behavior research. Although considerable effort has been directed toward investigating the relationship between music and dance, the synthesis of appropriate dance choreography based on music remains an open problem. There are two main challenges: 1) how to choose appropriate dance figures, i.e., groups of steps that are named and specified in technical dance manuals, in accordance with music and 2) how to artistically enhance choreography in accordance with music. To solve these problems, in this paper, we propose a music-oriented dance choreography synthesis method using a long short-term memory (LSTM)-autoencoder model to extract a mapping between acoustic and motion features. Moreover, we improve our model with temporal indexes and a masking method to achieve better performance. Because of the lack of data available for model training, we constructed a music-dance dataset containing choreographies for four types of dance, totaling 907,200 frames of 3D dance motions and accompanying music, and extracted multidimensional features for model training. We employed this dataset to train and optimize the proposed models and conducted several qualitative and quantitative experiments to select the best-fitted model. Finally, our model proved to be effective and efficient in synthesizing valid choreographies that are also capable of musical expression.
High-Throughput, Automated Protein A Purification Platform with Multiattribute LC–MS Analysis for Advanced Cell Culture Process Monitoring (2016)

The levels of many product related variants observed during the production of monoclonal antibodies are dependent on control of the manufacturing process, especially the cell culture process. However, it is difficult to characterize samples pulled from the bioreactor due to the low levels of product during the early stages of the process and the high levels of interfering reagents. Furthermore, analytical results are often not available for several days, which slows the process development cycle and prevents "real time" adjustments to the manufacturing process. To reduce the delay and enhance our ability to achieve quality targets, we have developed a low-volume, high-throughput, and high-content analytical platform for at-line product quality analysis. This workflow includes an automated, 96-well plate protein A purification step to isolate antibody product from the cell culture fermentation broth, followed by rapid, multiattribute LC-MS analysis. We have demonstrated quantitative correlations between particular process parameters with the levels of glycosylated and glycated species in a series of small scale experiments, but the platform could be used to monitor other attributes and applied across the biopharmaceutical industry.
<i>R</i>ecognition of <i>in</i>dividual <i>g</i>enes in a single bacterial cell by fluorescence <i>in situ</i> hybridization – RING‐FISH (2003)

Fluorescence in situ hybridization (FISH) using rRNA targeted oligonucleotide probes is a standard method for identification of microorganisms in environmental samples. Apart from its value as a phylogenetic marker ribosomal RNA has always been the favoured target molecule for FISH because of its abundance in all cells, whereas plasmids and DNA were regarded as unsuitable targets because of their low copy number. Here we present an improved FISH technique, which is based on polynucleotide probes. It goes beyond the detection of high copy intracellular nucleic acids such as rRNA (up to 10(4)-10(5) copies per cell) and allows for the first time the in situ detection of individual genes or gene fragments on plasmids (10(1)-10(3) copies per cell) and chromosomal DNA (<10 copies per cell) in a single cell. Using E. coli as model organism we were able to detect in situ cells harbouring the antibiotic resistance gene beta lactamase on high, medium and low copy plasmids as well as the chromosomal encoded housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Furthermore, we detected the prepilin peptidase gene xpsO in the plant pathogen Xanthomonas campestris in situ. Because of the characteristic hybridization signal obtained with this method--a halo-like, ring-shaped concentration of fluorescence in the cell periphery--we coined the term RING-FISH (recognition of individual genes) to differentiate it from conventional FISH.
Adjustable Hyperthermia Response of Self‐Assembled Ferromagnetic Fe‐MgO Core–Shell Nanoparticles by Tuning Dipole–Dipole Interactions (2012)

Abstract The Fe‐MgO core‐shell morphology is proposed within the single‐domain nanoparticle regime as an enhanced magnetically driven hyperthermia carrier. The combinatory use of metallic iron as a core material together with the increased particle size (37–65 nm) triggers the tuning of dipolar interactions between particles and allows for further enhancement of their collective heating efficiency via concentration control. A theoretical universal estimation of hysteresis losses reveals the role of dipolar interactions on heating efficiency and outlines the strong influence of coupling effects on hyperthermia opening a novel roadmap towards multifunctional heat‐triggered theranostics particles.
Sodium-Promoted Condensation of Organic Halides and Carbonyl Compounds (1954)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSodium-Promoted Condensation of Organic Halides and Carbonyl CompoundsCharles E. Frank and Walter E. FosterCite this: Ind. Eng. Chem. 1954, 46, 5, 1019–1021Publication Date (Print):May 1, 1954Publication History Published online1 May 2002Published inissue 1 May 1954https://pubs.acs.org/doi/10.1021/ie50533a056https://doi.org/10.1021/ie50533a056research-articleACS PublicationsRequest reuse permissionsArticle Views60Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Real-time, three-dimensional localization of a Brockenbrough needle during transseptal catheterization using a nonfluoroscopic mapping system. (2006)

We describe a new technique that allows real-time, three-dimensional (3-D) localization of the Brockenbrough needle tip during transseptal catheterization using the EnSite NavX system. Transseptal catheterization has been traditionally performed using fluoroscopy, and recently, with the use of intracardiac echocardiography. However, even intracardiac echocardiography has the limitation of providing only 2-D views limited to the ultrasound plane. By displaying the transseptal needle on the EnSite NavX system, we achieved real-time 3-D localization of the needle tip within the right atrial geometry and found accurate visual correlation between fluoroscopy, intracardiac echocardiography and nonfluoroscopic 3-D cardiac mapping. This study suggests that the EnSite NavX system is able to provide 3-D localization of the transseptal needle during transseptal catheterization, and may be a useful imaging modality in this procedure.
Application of Phase Change Materials and PCM slurries for thermal energy storage (2006)

The idea to use phase change materials (PCM) for the purpose of storing thermal energy is to make use of the latent heat of a phase change, usually between the solid and the liquid state. Since a phase change involves a large amount of latent energy at small temperature changes, PCMs are used for temperature stabilization and for storing heat with
Enhancement of tumor-associated antigen-specific T cell responses by radiofrequency ablation of hepatocellular carcinoma (2012)

Although RFA can enhance various TAA-specific T cell responses and the T cells induced contribute to the HCC recurrence-free survival of patients, besides immunosuppression by MDSCs, the memory phenotype and lifetime of TAA-specific T cells are not sufficient to prevent HCC recurrence completely. Additional treatments by vaccine or immunomodulatory drugs might be useful to improve the immunological effect of RFA.
Comparison of database replication techniques based on total order broadcast (2005)

In this paper, we present a performance comparison of database replication techniques based on total order broadcast. While the performance of total order broadcast-based replication techniques has been studied in previous papers, this paper presents many new contributions. First, it compares with each other techniques that were presented and evaluated separately, usually by comparing them to a classical replication scheme like distributed locking. Second, the evaluation is done using a finer network model than previous studies. Third, the paper compares techniques that offer the same consistency criterion (one-copy serializability) in the same environment using the same settings. The paper shows that, while networking performance has little influence in a LAN setting, the cost of synchronizing replicas is quite high. Because of this, total order broadcast-based techniques are very promising as they minimize synchronization between replicas.