gebhart/patrepeval huggingface dataset:
16S rRNA Gene-Based Oligonucleotide Microarray for Environmental Monitoring of the Betaproteobacterial Order “<i>Rhodocyclales</i>” (2005)

For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultured and as yet uncultured members of the "Rhodocyclales." The multiple nested probe set was evaluated for microarray hybridization with 16S rRNA gene PCR amplicons from 29 reference organisms. Subsequently, the RHC-PhyloChip was successfully used for cultivation-independent "Rhodocyclales" diversity analysis in activated sludge from an industrial wastewater treatment plant. The implementation of a newly designed "Rhodocyclales"-selective PCR amplification system prior to microarray hybridization greatly enhanced the sensitivity of the RHC-PhyloChip and thus enabled the detection of "Rhodocyclales" populations with relative abundances of less than 1% of all bacteria (as determined by fluorescence in situ hybridization) in the activated sludge. The presence of as yet uncultured Zoogloea-, Ferribacterium/Dechloromonas-, and Sterolibacterium-related bacteria in the industrial activated sludge, as indicated by the RHC-PhyloChip analysis, was confirmed by retrieval of their 16S rRNA gene sequences and subsequent phylogenetic analysis, demonstrating the suitability of the RHC-PhyloChip as a novel monitoring tool for environmental microbiology.
Effects of Low-Intensity Ultrasound on Chondrogenic Differentiation of Mesenchymal Stem Cells Embedded in Polyglycolic Acid: An <i>in Vivo</i> Study (2006)

In this study we investigated the effects of LIUS on chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSC). Our hypothesis is that LIUS may be a noninvasively effective stimulant to a biological system in vivo by turning on differentiation of MSCs and promotion of chondrogenesis. MSCs were isolated from the bone marrow of New Zealand white rabbits and cultured in monolayer for 2 weeks. They were then harvested and seeded into polyglycolic acid (PGA) non-woven mesh at a number of 5 x 10(6) cells. Cultured with a chondrogenic-defined media for 1 week, the PGA/MSCs constructs (n = 4) were implanted subcutaneously in the back of nude mice (n = 9, each group). The ultrasound (US) group received US stimulation at a frequency of 0.8 MHz and intensity of 200 mW/cm(2) for 10 min every day up to 4 weeks, while the control group had no US stimulation. Analyses of histological, immunohistochemical, biochemical, and mechanical characteristics were made at 1, 2, and 4 weeks post-stimulation, respectively. Total DNA contents showed no significant difference between the two groups. Total collagen and glycosaminoglycan (GAG) increased more significantly in the US-stimulated group than in the control. Histology of Safranin O/Fast green confirmed more intense and spreading extracellular matrix (ECM) at 2 and 4 weeks in the US-stimulated specimens. Mechanical tests exhibited that compressive strengths were also significantly higher in the US-stimulated cells at later times. This study strongly suggests that it may be possible for ultrasound to have some stimulatory effects in vivo on the chondrogenesis of MSCs.
Pulmonary tumor embolization after peritoneovenous shunting for malignant ascites (1988)

An 85-year-old woman with intractable malignant ascites secondary to ovarian carcinoma underwent peritoneovenous shunting (Denver shunt) in an attempt to alleviate the ascites. Implantation of the shunt resulted in massive embolization of tumor cells to the pulmonary vasculature. Postoperatively, she developed increasing hypoxia with progressive rises in pulmonary artery pressure, and died 48 hours after surgery as a result of occlusion of the pulmonary vascular bed by tumor emboli. This is the sixth reported instance of massive tumor embolization to the pulmonary circulation in patients with peritoneovenous shunting for malignant ascites.
Component-based handprint segmentation using adaptive writing style model (1996)

Building upon the utility of connected components, NIST has designed a new character segmentor based on statistically modeling the style of a person's handwriting. Simple spatial features (the thickness of the pen stroke and the height of the handwriting) capture the characteristics of a particular writer's style of handprint, enabling the new method to maintain a traditional character-level segmentation philosophy without the integration of recognition or the use of oversegmentation and linguistic postprocessing. Estimates for stroke width and character height are used to compute aspect ratio and standard stroke count features that adapt to the writer's style at the field level. The new method has been developed with a predetermined set of fuzzy rules making the segmentor much less fragile and much more adaptive, and the new method successfully reconstructs fragmented characters as well as splits touching characters. The new segmentor was integrated into the NIST public domain Form-Based Handprint Recognition System and then tested on a set of 490 Handwriting Sample Forms found in NIST Special Database 1 9. When compared to a simple component-based segmentor, the new adaptable method improved the overall recognition of handprinted digits by 3.4% and field level recognition by 6.9%, while effectively reducing deletion errors by 82%. The same program code and set of parameters successfully segments sequences of uppercase and lowercase characters without any contextbased tuning. While not as dramatic as digits, the recognition of uppercase and lowercase characters improved by 1.7% and 1.3% respectively. The segmentor maintains a relatively straight-forward and logical process flow avoiding convolutions of encoded exceptions as is common in expert systems. As a result, the new segmentor operates very efficiently, and throughput as high as 362 characters per second can be achieved. Letters and numbers are constructed from a predetermined configuration of a relatively small number of strokes. Results in this paper show that capitalizing on this knowledge through the use of simple adaptable features can significantly improve segmentation, whereas recog- nition-based and oversegmentation methods fail to take advantage of these intrinsic qualities of handprinted characters.
Electrochemical Impedance Spectroscopy Measurement of Lithium Battery Based on Lock-in amplifier (2019)

Electrochemical impedance spectroscopy (EIS) is a useful technique in research on lithium battery. EIS is often measured by electrochemical workstations whose price is extremely high. This paper proposed a new method for measuring EIS of lithium battery, which was based on lock-in amplifier and some auxiliary circuits. The lock-in amplifier can achieve measuring ultra-low frequency small signal and the auxiliary circuits can complete the expansion of the output current and avoid the influence of the output impedance of lock-in amplifier. This method can measure EIS of lithium batteries accurately while avoid using expensive equipment such as electrochemical workstation.
Brucella melitensis Methionyl-tRNA-Synthetase (MetRS), a Potential Drug Target for Brucellosis (2016)

We investigated Brucella melitensis methionyl-tRNA-synthetase (BmMetRS) with molecular, structural and phenotypic methods to learn if BmMetRS is a promising target for brucellosis drug development. Recombinant BmMetRS was expressed, purified from wild type Brucella melitensis biovar Abortus 2308 strain ATCC/CRP #DD-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-tRNA-synthetase inhibitors of the blood stage form of Trypanosoma brucei. Three compounds showed appreciable shift of denaturation temperature and were selected for further studies on inhibition of the recombinant enzyme activity and cell viability against wild type B. melitensis strain 16M. BmMetRS protein complexed with these three inhibitors resolved into three-dimensional crystal structures and was analyzed. All three selected methionyl-tRNA-synthetase compounds inhibit recombinant BmMetRS enzymatic functions in an aminoacylation assay at varying concentrations. Furthermore, growth inhibition of B. melitensis strain 16M by the compounds was shown. Inhibitor-BmMetRS crystal structure models were used to illustrate the molecular basis of the enzyme inhibition. Our current data suggests that BmMetRS is a promising target for brucellosis drug development. However, further studies are needed to optimize lead compound potency, efficacy and safety as well as determine the pharmacokinetics, optimal dosage, and duration for effective treatment.
Guideline limit volumes for dosing animals in the preclinical stage of safety evaluation (1995)

Guideline limit volumes for dosing laboratory animals by oral and parenteral routes in the preclinical stage of safety evaluation were agreed following discussions by the Toxicology Subcommittee of the Association of the British Pharmaceutical Industry. The guideline values represent common practice rather than absolute maxima. Whilst the guideline values are expected to be of value to scientists and technical staff involved in study design and applicable to the majority of routine safety evaluation studies, scope remains to make the case for special investigations. The need to carefully consider animal welfare and physiology, current legislation, the use of appropriate dosing technology and formulations, and other practical aspects of study conduct, is emphasised.
Signal transduction by G‐proteins, Rho‐kinase and protein phosphatase to smooth muscle and non‐muscle myosin II (2000)

We here review mechanisms that can regulate the activity of myosin II, in smooth muscle and non-muscle cells, by modulating the Ca2+ sensitivity of myosin regulatory light chain (RLC) phosphorylation. The major mechanism of Ca2+ sensitization of smooth muscle contraction and non-muscle cell motility is through inhibition of the smooth muscle myosin phosphatase (MLCP) that dephosphorylates the RLC in smooth muscle and non-muscle. The active, GTP-bound form of the small GTPase RhoA activates a serine/threonine kinase, Rho-kinase, that phosphorylates the regulatory subunit of MLCP and inhibits phosphatase activity. G-protein-coupled release of arachidonic acid may also contribute to inhibition of MLCP acting, at least in part, through the Rho/Rho-kinase pathway. Protein kinase C(s) activated by phorbol esters and diacylglycerol can also inhibit MLCP by phosphorylating and thereby activating CPI-17, an inhibitor of its catalytic subunit; this mechanism is independent of the Rho/Rho-kinase pathway and plays only a minor, transient role in the G-protein-coupled mechanism of Ca2+ sensitization. Ca2+ sensitization by the Rho/Rho-kinase pathway contributes to the tonic phase of agonist-induced contraction in smooth muscle, and abnormally increased activation of myosin II by this mechanism is thought to play a role in diseases such as high blood pressure and cancer cell metastasis.
Trends in admission and case fatality for acute malnutrition (Kingston 1982-1991). (1996)

The case fatality ratio (CFR) in acute protein-energy malnutrition (PEM) achieved in the Tropical Metabolism Research Unit (TMRU) was compared with that of other tertiary care facilities in Kingston. Trends in admission and fatality rates, case severity and complications were also examined. From ward admission registers for Bustamante Hospital for Children (BHC), the University Hospital of the West Indies (UHWI), children's wards and the TMRU all cases of PEM admitted from 1982 through 1991 were enumerated and there was a docket search for random subsamples. Ten-year mean CFR% for BHC was 8.8 (n = 1948); for UHWI wards 5.5 (n = 658); for TMRU 7.1 (n = 662). BHC has the least restrictions on admission and showed most clearly that the peak time in Kingston for admission of PEM was around 1985, falling to a minimum in 1988-1990 and rising again in 1991; however, the other sites also showed similar trends. BHC had a range of CFR% p.a. of 20.0 to 3.0, with a striking fall in the second half of the decade. There was no temporal CFR trend for the UHWI or TMRU. The latter institution had the highest proportion of admissions with marasmic-kwashiorkor and the lowest proportion with recorded infection. The annual variation in numbers of PEM deaths at BHC was best accounted for by (a) percentage change in consumer price index and (b) percentage change in the US$ value of the Jamaican $, in the preceding year, and (c) annual number of admissions, together. Generally, our findings suggest a minor role for expert in-patient management in reducing deaths from PEM.
Optimizing 3D Virtual Fitting Mirror Using Augmented Reality Technology under Internet Plus (2022)

At present, with the ups and downs of the “Internet Plus” era, all things realize intelligent interconnection. Internet technologies such as big data and artificial intelligence have exploded, playing an important role as a bridge in the era of “Internet Plus” and bringing a profound revolution to human life. In order to solve the personalized and diversified dressing needs of consumers in the new retail mode, enhance the reality of 3D clothing model, and make up for the shortcomings of the user experience in the clothing e-commerce industry, this paper studies the 3D fitting mirror based on Kinect, and proposes the use of Random Sample Consensus algorithm to optimize the virtual fitting mirror and improve the user's fitting experience.
Maximum likelihood based methods for OFDM intercarrier spacing characterization (2008)

One of the main task to be done by a cognitive receiver is to sensing its spectral environment in order to distinguish surrounding systems from each others. Actually most systems (such as WiMAX, WiFi, DVBT) are based on OFDM modulations but differ from their intercarrier spacing used in OFDM modulation. Therefore carrying out accurate intercarrier spacing estimator is a crucial step in cognitive radio. In this paper, we propose a new efficient algorithm to estimate the intercarrier spacing based on maximum-likelihood principle. Its performance is analysed through numerical simulations and compared to standard existing approaches.
Deep level transient spectroscopic and Hall effect investigation of the position of the vanadium acceptor level in 4H and 6H SiC (1996)

Hall effect, deep level transient spectroscopy (DLTS) and optical absorption measurements were employed in concert to determine the position of the vanadium acceptor level in vanadium and nitrogen doped 6H and 4H SiC. Hall effect results indicate that the acceptor position in 4H SiC is at 0.80 eV beneath the conduction band edge, and 0.66 eV for the 6H polytype. The DLTS signature of the defect in the 4H polytype showed an ionization energy of 0.80 eV and a capture cross section of 1.8×10−16 cm−2. The optical absorption measurements proved that the levels investigated are related to isolated vanadium, and therefore the vanadium acceptor level. Based on the DLTS measurements and secondary ion mass spectroscopy data, the maximum solubility of vanadium in SiC was determined to be 3.0×1017 cm−3. At these incorporation limits and with the depth of the level, the vanadium acceptor level could be used in the creation of semi-insulating silicon carbide.
An efficient multicast protocol in mobile IPv6 networks (2004)

Mobile IPv6 provides two basic schemes called the remote subscription and home subscription to provide multicast service for mobile hosts. These two algorithms have complementary advantages. In this paper, we propose a new scheme called the previous network subscription, which can provide a low handover delay for real time multicast applications. We also propose a new multicast routing protocol called RHMoM (region based hierarchical mobile multicast) by combining the previous network subscription and the home subscription. RHMoM uses two kinds of agents and employs the region based hierarchical architecture to limit the reconstruction frequency of the multicast tree and optimize the delivery path. The simulation results show that RHMoM has better performance than the other mobile multicast solutions in mobile IPv6 network.
Dot immunobinding assay of viral antigen and antibodies. (1986)

A dot-immunobinding assay for the rapid and specific detection of viral antibody as well as the identification of a virus is described. Based on the use of nitrocellulose membranes for the adsorption of viral protein, this test may be used to detect the presence of virus antibody or identify an isolate within 4 to 6 hours. Use of goat anti-human IgG-alkaline phosphatase followed by a naphthol-AS-MX-phosphate: Fast Red substrate permits visual detection of a positive reaction. The use of psoralen inactivated herpes B virus permits the incorporation of this virus into the battery of test antigens.
When the Medium is the Message: Corporate Buybacks as Signals (1990)

figures were $3.4 billion through repurchases and $29.5 billion through dividends.Id.For a six week period near the end of 1989, buybacks of up to $20 billion
The practice of silviculture : applied forest ecology (1997)

Partial table of contents: INTRODUCTION TO SILVICULTURE. Silviculture and Its Place in Forestry. Stand Dynamics. TENDING AND INTERMEDIATE CUTTING. The Response of Individual Trees to Thinning and Pruning. Management of Growth and Stand Yield by Thinning. Methods and Application of Thinning. Release Operations and Herbicides. REGENERATION. Ecology of Regeneration. Site Classification and Species Selection. Artificial Regeneration. STAND DEVELOPMENT AND STRUCTURE. The Silviculture of Pure Even-Aged Stands. Vegetatively Regenerated Stands. Double-Cohort Pure Stands Regenerated by Partial Cutting. Silviculture of Pure Uneven-Aged Stands. Stands of Mixed Species. SILVICULTURAL MANAGEMENT OBJECTIVES. Timber Management. Silvicultural Management of Watershed Ecosystems. Silvicultural Control of Damaging Agencies. Agroforestry. Appendices. Index.
Persistent memory over fabric (PMoF) (2017)

Persistent Memory (PM) is an emerging family of technologies that are: persistent; byte addressable; and respond in near-memory speeds. PM devices, also referred to as non-volatile DIMMs or NVDIMMs, connect to the low-latency CPU memory interconnect. PM-based solutions can achieve local persistency within a micro second, which is two orders-of-magnitude faster compared to modern Flash solutions [1].
Long noncoding RNA expression profiles in sub-lethal heat-treated hepatoma carcinoma cells (2017)

lncRNA expression data in sub-lethally heat-treated HCC cells will provide important insights about lncRNAs' contribution to HCC recurrence after RFA treatment.
Behavioral phenotypes and pharmacology in genetic mouse models of Parkinsonism (2006)

Prior to the discovery of genes associated with familial forms of Parkinson's disease, animal models of Parkinson's disease mainly consisted of toxin models based exclusively on the degeneration of nigrostriatal dopamine neurons. These traditional models have provided valuable insight into symptomatic treatments for Parkinson's disease; however, they lack the broad extra-nigral pathology and the progression that is observed in the disease. The novel genetic mouse models recently generated are advantageous because they have mutations that are known to cause familial Parkinson's disease and thus they have good construct validity. To maximize the utility of these models, a thoughtful phenotypical characterization is important. Our laboratory has assembled a battery of behavioral tests to assess sensorimotor function in genetic mouse models of Parkinsonism. This review discusses the sensitivity of these tests in different genetic mice in addition to their behavioral response to dopamine agonists.
Carbohydrate-binding modules promote the enzymatic deconstruction of intact plant cell walls by targeting and proximity effects (2010)

Cell wall degrading enzymes have a complex molecular architecture consisting of catalytic modules and noncatalytic carbohydrate-binding modules (CBMs). The function of CBMs in cell wall degrading processes is poorly understood. Here, we have evaluated the potential enzyme-targeting function of CBMs in the context of intact primary and secondary cell wall deconstruction. The capacity of a pectate lyase to degrade pectic homogalacturonan in primary cell walls was potentiated by cellulose-directed CBMs but not by xylan-directed CBMs. Conversely, the arabinofuranosidase-mediated removal of side chains from arabinoxylan in xylan-rich and cellulose-poor wheat grain endosperm cell walls was enhanced by a xylan-binding CBM but less so by a crystalline cellulose-specific module. The capacity of xylanases to degrade xylan in secondary cell walls was potentiated by both xylan- and cellulose-directed CBMs. These studies demonstrate that CBMs can potentiate the action of a cognate catalytic module toward polysaccharides in intact cell walls through the recognition of nonsubstrate polysaccharides. The targeting actions of CBMs therefore have strong proximity effects within cell wall structures, explaining why cellulose-directed CBMs are appended to many noncellulase cell wall hydrolases.
Injury of the anterior cruciate ligament: The role of collagenase in ligament degeneration (1989)

Rapid degeneration of the anterior cruciate ligament (ACL) has been observed following acute ACL rupture. An understanding of this process might explain some of the poor clinical results of primary ACL repair. We created a surgical rabbit model of acute ACL injury and developed an in vitro assay for collagenase activity in the ACL and menisci. Microscopic evaluation revealed a rapidly degenerative process in injured ACLs, with loss of cellularity and matrix organization. This was associated with a significant increase in collagenase activity and a decrease in total collagen of the injured ACLs as compared with sham-operated controls. These findings confirm the observation that cut ACL ligament ends rapidly degenerate. This degenerative process might be partly due to a response of cells intrinsic to the ACL to injury. Left unchecked, this process may be detrimental to surgical attempts for primary ACL repair.
Type-based hot swapping of running modules (extended abstract) (2001)

While dynamic linking has become an integral part of the run-time execution of modem programming languages, there is increasing recognition of the need for support for hot swapping of running modules, particularly in long-lived server applications. The interesting challenge for such a facility is to allow the new module to change the types exported by the original module, while preserving type safety. This paper describes a type-based approach to hot swapping running modules. The approach is based on a reflective mechanism for dynamically adding type sharing constraints to the type system, realized by programmer-defined version adapters in the run-time.