gebhart/patrepeval huggingface dataset:
Natural killer cell cytokine production, not cytotoxicity, contributes to resistance against blood-stage Plasmodium chabaudi AS infection (1997)

Our recent study showed that IL-12 treatment of susceptible A/J mice induces Th1-mediated, protective immunity against lethal blood-stage Plasmodium chabaudi AS infection. To further understand the mechanism of this protection, we examined NK cell cytotoxic (NKCC) and cytokine secretory functions in untreated and IL-12-treated A/J mice, along with resistant C57BL/6 (B6) mice. Normal A/J mice receiving six daily doses of 0.1 microg IL-12 exhibited significant increases in NKCC in total spleen cell populations. Defective NKCC evident in vitro in enriched NK cells from infected A/J mice was corrected by addition of 10 ng/ml IL-12 and was comparable with that seen in B6 mice. In vivo and in vitro analyses revealed that enriched NK cells from day 6 infected A/J mice were defective not only in NKCC, but also in IFN-gamma, and to a certain extent, TNF-alpha secretion, which could also be corrected by IL-12 treatment. Depletion of NK cells from resistant B6 mice resulted in a more severe course of infection, while NK cell-depleted, IL-12-treated A/J mice had significantly higher parasitemia, as well as 100% mortality, suggesting the importance of NK cells in IL-12-mediated protection. NKCC-defective bg/bg mice produced optimum IFN-gamma and TNF-alpha and recovered from infection similar to bg/+ controls; in vivo depletion of these cytokines resulted in significantly higher parasitemia early in infection. Based on these results, we conclude that IFN-gamma, and possibly TNF-alpha, secretion by NK cells during early infection plays a major role in protective immunity to blood-stage malaria.
Detection of specific proteins by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) using gold cluster labelled antibodies (2005)

Laser ablation ICP-MS (LA-ICP-MS) has recently been applied to the determination of protein-bound metals using gels and gel blots. As all structural information is lost in the plasma flame, a direct determination of specific proteins is not possible. To overcome this problem, a novel application of LA-ICP-MS is presented, which takes advantage of the well established Western-Blotting-Technique to specifically detect proteins by the use of antibodies conjugated to gold clusters. After protein separation by regular 1D SDS-PAGE and transfer to a blotting membrane, the protein was marked by subsequent use of a primary antibody raised in rabbit and a mouse-anti-rabbit antibody covalently coupled to a gold cluster. We showed that the gold cluster could be detected by LA-ICP-MS. In comparison to single atoms, metal clusters increase the sensitivity in correlation to the number of metal atoms in the cluster. Quantification of the labelled antibodies was carried out by calibration using matrix matched standards. By using LA-ICP-MS, a detection limit of about 0.20 amol for gold cluster labelled antibody as well as excellent linearity was reached. As LA-ICP-MS allows the simultaneous multielement determination of protein-bound metal(loid)s this method is well suited to studies that require both the simultaneous quantification of a specific protein and the protein-bound metal(loid)s. This method is suitable for any protein for which a primary antibody is available. Here we applied it to analysis of the Mre11 protein in crude lysates of CHO-K1 fibroblasts. This protein is essential for mediating genome stability in mammalian cells and involved in DNA-repair and recombination.
Dications of Fluorenylidenes. Contribution of Magnetic and Structural Effects to the Antiaromaticity of Fluorenylidene Dications with Cyclic Substituents (2002)

The antiaromaticity of fluorenylidene dications 1-5, which contain cyclic cationic substituents, has been examined using magnetic criteria, NICS and magnetic susceptibility, and by structural criteria, HOMA. The magnetic criteria, including proton chemical shifts, strongly support the antiaromaticity of the fluorenyl ring system of these dications. HOMA values are a very insensitive measure of structural effects in polycyclic ring systems because they reflect the inability of the fused ring systems to respond to changes in aromaticity/antiaromaticity. Finally, in these systems, the interaction between the ring systems appears to occur primarily through a type of hyperconjugation, as demonstrated by a decrease in the bond lengths for the bonds connecting the ring systems. In conjunction with the evaluation of magnetic effects, the quality of the calculation of (1)H and (13)C NMR shifts was assessed by comparison with experimental data. The calculation of (13)C NMR shifts was excellent in all methods examined, but the quality of (1)H NMR shifts was substantially poorer in calculations using the IGLO method, basis set DZ or II. The CSGT method gives a superior correlation between experimental and calculated (1)H NMR shifts.
Continuous-Flow Assay for Urinary Oxalate Using Immobilised Oxalate Oxidase (1985)

A continuous-flow assay for measuring oxalate in urine is described. Covalently attached oxalate oxidase (EC 1.2.3.4) is used to oxidize the oxalate anion to carbon dioxide and hydrogen peroxide. The formed hydrogen peroxide is measured colorimetrically (A580) with an established reaction using horseradish peroxidase (EC 1.11.17), 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-dimethylaminobenzoic acid (DMAB). Ascorbate interference is eliminated by treating the urine sample with sodium nitrite prior to assaying. The assay is accurate (mean recovery of added oxalate in spiked urine sample is 93 +/- 11%), sensitive (detection limit 1.0 mumol/L), reproducible (within-batch CV 3.5%; between-batch CV 5%) and relatively rapid (15 samples/h). This assay correlates well (R = 0.99) with another established enzymatic method (using oxalate decarboxylase).
Chemotherapeutic Nitrofurans. IV.<sup>1</sup> Some Derivatives of 1-Amino-2-imidazolidinone, 1-Amino-2-pyrrolidinone and 3-Amino-2-thiazolidinone (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemotherapeutic Nitrofurans. IV.1 Some Derivatives of 1-Amino-2-imidazolidinone, 1-Amino-2-pyrrolidinone and 3-Amino-2-thiazolidinoneJulian G. Michels and Gabriel GeverCite this: J. Am. Chem. Soc. 1956, 78, 20, 5349–5351Publication Date (Print):October 1, 1956Publication History Published online1 May 2002Published inissue 1 October 1956https://pubs.acs.org/doi/10.1021/ja01601a052https://doi.org/10.1021/ja01601a052research-articleACS PublicationsRequest reuse permissionsArticle Views341Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
pH-sensitive polycarbonate micelles for enhanced intracellular release of anticancer drugs: a strategy to circumvent multidrug resistance (2014)

In this study, we have synthesized two novel amphiphilic diblock copolymers with aldehyde groups via organocatalytic ROP of a functionalized cyclic carbonate monomer (MTC-Bz) using polyethylene oxide (PEG) as the macroinitiator. The polymers were covalently conjugated with an anti-tumor drug doxorubicin (DOX) via a pH-sensitive Schiff-base linkage. The resulting conjugates formed micelles in phosphate-buffered saline (PBS) (pH 7.4) with an average size of about 100 nm and narrow size distribution. The surface charge of the micelles was close to zero. The micelles were stable in both PBS and cell culture media containing 10% FBS up to 5 days. The results obtained from the in vitro release study indicated that DOX release from the micelles was pH-dependent, being faster at pH 5.0 (the endolysosomal environment) than pH 7.4 (the extracellular environment). Human breast cancer MCF-7 cells and DOX-resistant MCF-7/Adr cells were employed to investigate the cellular uptake and cytotoxicity of DOX-loaded micelles. The confocal microscopy and flow cytometry studies showed that the uptake of DOX-loaded micelles by MCF-7 cells was similar to that of free DOX. In sharp contrast, the uptake of DOX-loaded micelles by MCF-7/Adr cells was significantly higher than that of free DOX. The polymers showed no toxicity to MCF-7 and MCF-7/Adr cells. The DOX-loaded micelles killed the cells efficiently. In particular, they were more potent against drug-resistant MCF-7/Adr cells than free DOX due to the higher cellular drug accumulation and pH-triggered intracellular drug release, providing a strategy to navigate around drug resistance. These DOX-conjugated micelles can be a promising carrier for the delivery of anticancer drugs with amine functional groups.
GHRH-induced GH response in patients with senile dementia of the Alzheimer type (1988)

To clarify the functional state of the somatotropinergic system at the hypothalamo-hypophyseal level in senile dementia of the Alzheimer type, the GHRH test was performed in three groups of subjects: a) healthy elderly subjects; b) early onset senile dementia patients; and c) late onset senile dementia patients. Intravenous administration of GHRH(1-44)NH2 (100 micrograms) elicited a marked plasma GH response with a maximum peak (709.54 +/- 259.0 pmol/l; P less than 0.005) 60 min after injection in patients with early onset senile dementia, but no significant response was detected in the other two groups. Electroencephalographic recording showed that GHRH modifies brain bioelectrical activity, decreasing frequency (0.52 +/- 0.15 Hz) and increasing amplitude (8.25 +/- 4.5 microV) of the electroencephalogram basic rhythm. The evaluation of mental performance and behaviour with a battery of different tests for mental assessment revealed that GHRH induces transient clinical changes in psychomotor behaviour. According to these results, it seems likely that the somatostatin deficiency reported in senile dementia of the Alzheimer type may account for the enhanced GHRH-induced GH response observed in patients with early onset senile dementia. In consequence, the GHRH test might constitute a useful antemortem marker for senile dementia of the Alzheimer type if the present results can be replicated in early stages of the disease.
Three methionine residues located within the regulator of conductance for K<sup>+</sup> (RCK) domains confer oxidative sensitivity to large‐conductance Ca<sup>2</sup><sup>+</sup>‐activated K<sup>+</sup> channels (2006)

Methionine-directed oxidation of the human Slo1 potassium channel (hSlo1) shifts the half-activation voltage by -30 mV and markedly slows channel deactivation at low concentrations of intracellular Ca2+ ([Ca2+]i). We demonstrate here that the contemporaneous mutation of M536, M712 and M739 to leucine renders the channel functionally insensitive to methionine oxidation caused by the oxidant chloramine-T (Ch-T) without altering other functional characteristics. Coexpression with the auxiliary beta1 subunit fails to restore the full oxidative sensitivity to this triple mutant channel. The Ch-T effect is mediated specifically by M536, M712 and M739 because even small changes in this residue combination interfere with the ability to remove the oxidant sensitivity following mutation. Replacement of M712 or M739, but not M536, with the hydrophilic residue glutamate largely mimics oxidation of the channel and essentially removes the Ch-T sensitivity, suggesting that M712 and M739 may be part of a hydrophobic pocket disrupted by oxidation of non-polar methionine to the more hydrophilic methionine sulfoxide. The increase in wild-type hSlo1 open probability caused by methionine oxidation disappears at high [Ca2+]i and biophysical modelling of the Ch-T effect on steady-state activation implicates a decrease in the allosteric coupling between Ca2+ binding and the pore. The dramatic increase in open probability at low [Ca2+]i especially within the physiological voltage range suggests that oxidation of M536, M712 or M739 may enhance the Slo1 BK activity during conditions of oxidative stress, such as those associated with ischaemia-reperfusion and neurodegenerative disease, or in response to metabolic cues.
Differentiëren in rekenonderwijs: afstemmen op onderwijsbehoeften (2019)

<p>De rekenmethode geeft vaak suggesties voor verwerking op meerdere niveaus, maar wat kun je als leerkracht zelf doen om je onderwijs beter af te stemmen op de onderwijsbehoefte van de kinderen? Hoe pak je differentiatie aan?<br /></p>
Attaining soft real-time constraint and energy-efficiency in web servers (2008)

M/M/1 queues have been traditionally used to model several systems like phone calls at a call center, banking services and so on. However, recent studies showed that it does not model properly a web server system. In this paper we investigate the impact of this assumption in providing timeliness constraint in an energy-efficient web server. Although energy efficiency is a key issue, it should not be attained at the expense of a poor quality of service. The work proposed here describes a technique that uses queueing theory results to balance energy consumption and adequate application response times in heterogeneous CPU-intensive server clusters. Moreover, we investigate the I/O impact on a purely CPU-oriented energy saving strategy. This proposal shows that the assumption of a Poisson process is a good approximation to model a web server.
Multiomic blood correlates of genetic risk identify presymptomatic disease alterations (2020)

Transitions from health to disease are characterized by dysregulation of biological networks under the influence of genetic and environmental factors, often over the course of years to decades before clinical symptoms appear. Understanding these dynamics has important implications for preventive medicine. However, progress has been hindered both by the difficulty of identifying individuals who will eventually go on to develop a particular disease and by the inaccessibility of most disease-relevant tissues in living individuals. Here we developed an alternative approach using polygenic risk scores (PRSs) based on genome-wide association studies (GWAS) for 54 diseases and complex traits coupled with multiomic profiling and found that these PRSs were associated with 766 detectable alterations in proteomic, metabolomic, and standard clinical laboratory measurements (clinical labs) from blood plasma across several thousand mostly healthy individuals. We recapitulated a variety of known relationships (e.g., glutamatergic neurotransmission and inflammation with depression, IL-33 with asthma) and found associations directly suggesting therapeutic strategies (e.g., Ω-6 supplementation and IL-13 inhibition for amyotrophic lateral sclerosis) and influences on longevity (leukemia inhibitory factor, ceramides). Analytes altered in high-genetic-risk individuals showed concordant changes in disease cases, supporting the notion that PRS-associated analytes represent presymptomatic disease alterations. Our results provide insights into the molecular pathophysiology of a range of traits and suggest avenues for the prevention of health-to-disease transitions.
Synthesis and analgesic activity of C-terminal fragment peptides of cholecystokinin. (1987)

Eleven C-terminal fragment peptides of cholecystokinin (CCK) which contain a O-sulfated tyrosine residue [CCK (27-32), CCK (27-31), CCK (27-31) amide, CCK (27-30), CCK (27-30) amide, CCK (27-29), CCK (27-29) amide, CCK (26-27), CCK (26-27) amide, CCK (25-27) and CCK (25-27) amide] were prepared by the solution method. The syntheses of these peptides were carried out by stepwise condensation in combination with fragment condensation. Analgesic effects of these fragment peptides were measured by means of the writhing test. The ED50 value of CCK (27-32) was 9.1 mg/kg and that of CCK (25-27) was 7.5 mg/kg, but the other synthetic peptides produced no statistically significant response at a dose of 8 or 10 mg/kg.
Guayule resin separation and purification (1992)

Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.
Burning Characteristics of Individual Douglas-Fir Trees in the Wildland/Urban Interface (2011)

"The Wildland/Urban Interface, in which homes are intermingled with forested areas, presents unique challenges to fire protection and fire prediction, owing to the different fuel loads, conditions, and terrain. Computer models that predict fire spread through such an area require data for multiple scales, from crown fire spread to the heat release rates and ignition conditions for individual trees, as well as an understanding of fire behavior and spread. This discussion investigates a means by which fire behavior for Douglas-fir trees can be determined from quantifiable characteristics, such as height and moisture content. Mass, flame height, peak heat release rate, and total energy can be estimated from these simple measurements. A time scale of 60 seconds, combined with a peak heat release rate estimated from tree size characteristics, provides an approximation of total energy that is within 11% of measured values. Pre-heating of trees with a low (2.5 kW/m2) radiant heat flux did not have a noticeable impact on the resulting heat release rate. In addition, fire spread between trees was highly dependent on the presence of ambient wind; in the absence of wind or wind-borne embers, the trees were very resistant to ignition even when in close proximity (3 spacing). With the addition of wind, the fire would spread, although the heat release rates were dramatically reduced for trees of sufficiently high moisture content (< 70%)."
Applications of a New Chemiluminometric Thyrotropin Assay to Subnormal Measurement* (1990)

A new immunochemiluminometric TSH assay (ICMA) was shown to offer improved analytical (+2 SD of zero) and functional (20% interassay coefficient of variation) sensitivity [0.003 vs 0.045 +/- 0.005 (+/- SE; range, 0.01-0.07); 0.018 vs. 0.23 +/- 0.02 (range, 0.10-0.35, mU/L); analytical vs. functional sensitivity limit for the ICMA vs. 10 other TSH immunometric assays, respectively]. The ICMA was used to study the physiological relationship between serum TSH and free T4 [as reflected by free T4 index (FT4I)] values at both steady state and 14 days after acute pharmacological T4 administration (3 mg oral T4 load plus 0.3 mg daily). At steady state, an inverse log/linear relationship was found between serum TSH and FT4I values (log TSH = 2.56 - 0.022 FT4I; r = 0.84; P less than 0.001). Ten to 14 days after acute T4 suppression in 5 euthyroid subjects, serum TSH/FT4I levels had plateaued after decreasing in parallel to the slope of the steady state relationship, suggesting that the degree of T4 suppression of TSH can be predicted from an individual's pituitary TSH/free T4 set-point and the magnitude of the serum T4 elevation achieved. Ambulatory and hospitalized patient sera, previously identified as having low (less than 0.1 mU/L) TSH levels by a less sensitive assay, were restudied by the TSH ICMA. Normal TSH values ranged from 0.39-4.6 mU/L, whereas the majority of hyperthyroid patients [52 of 54 (96% ambulatory) and 22 of 23 (96%, hospitalized)] had undetectable (less than 0.005 mU/L), basal TSH levels and absent TRH stimulated TSH responses. In contrast, most (32 of 37; 86%) of hospitalized nonhyperthyroid patients with low (less than 0.1 mU/L) TSH values due to nonthyroidal illness or glucocorticoid treatment had detectable (greater than 0.01 mU/L) basal and TRH stimulated TSH levels. The positive relationship between basal and TRH-stimulated TSH levels was shown to extend down to the detectability limit of the assay (0.005 mU/L), which further supported the authenticity of the subnormal TSH ICMA measurements. The new TSH ICMA is considered to represent the first of a third generation of clinical TSH assays, since it has a functional (interassay) sensitivity that is 2 orders of magnitude greater than that of typical first generation TSH RIAs and 1 order of magnitude greater than current second generation TSH immunometric methods. Such third generation TSH assays will facilitate both the optimization of T4 therapy as well as the diagnosis of hyperthyroidism in hospitalized patients with nonthyroidal illness.
Intent Inference with Path Prediction (2006)

In today's air-traffic management system, the intent of an aircraft is revealed in its flight plan and a pilot's adherence to published navigation routes. In a future free-flight environment, aircraft might be allowed to fly any route they choose. Intent will likely be broadcast in the form of trajectory change points (TCPs), for instance, up to four TCPs in an automatic dependent surveillance-broadcast message. However, if broadcast TCPs do not accurately represent intent, do not exist, or do not get received, then a nearby aircraft or ground-monitoring system has a need to infer the pilot's intent in real time. In this paper, a method of inferring intent, which is based on artificial intelligence models and a process for best fitting an intent model to observed aircraft motion, is investigated. Horizontal, vertical, and speed dimensions are first investigated independently, and then combined, including sequences of actions, to fully explain the three-dimensional guidance and navigation plan of an aircraft. Finally, the inferred intent is used as a basis to predict a path from the current location of the aircraft into the future.
Progressive geometry compression for meshes (2002)

A novel progressive geometry compression scheme is presented in this paper. In this scheme, a mesh is represented as a base mesh followed by some groups of vertex split operations using an improved simplification method in which each level of the mesh can be refined into the next level by carrying out a group of vertex split operations in any order. Consequently, the progressive mesh (PM) representation can be effectively encoded by permuting the vertex split operations in each group. Meanwhile, a geometry predictor using the Laplacian operator is designed to predict each new vertex position using its neighbours. The correction is quantized and encoded using a Huffman coding scheme. Experimental results show that our algorithm obtains higher compression ratios than previous work. It is very suitable for the progressive transmission of geometric models over the Internet.
Immunosuppression‐induced leukoencephalopathy from tacrolimus (FK506) (1996)

Tacrolimus (FK506) has recently been approved for immunosuppression in organ transplantation, although its use is accompanied by a wide spectrum of neurotoxic side effects. We describe the clinical, radiological, and pathological features of 3 cases of tacrolimus-related leukoencephalopathy. The syndrome of immunosuppression-related leukoencephalopathy is proposed as an uncommon neurological syndrome occurring in patients with organ transplants involving demyelination, in particular in the parieto-occipital region and centrum semiovale. Although the syndrome is not associated with a particular (absolute) serum level of tacrolimus, it resolves spontaneously upon decreasing the dose. The tacrolimus-related syndrome has a similar radiographic and pathologic appearance as the analogous syndrome that occurs in patients taking cyclosporine.