gebhart/patrepeval huggingface dataset:
Fibroblast Growth Factor and Cyclic AMP (cAMP) Synergistically Activate Gene Expression at a cAMP Response Element (1994)

Growth factors and cyclic AMP (cAMP) are known to activate distinct intracellular signaling pathways. Fibroblast growth factor (FGF) activates ras-dependent kinase cascades, resulting in the activation of MAP kinases, whereas cAMP activates protein kinase A. In this study, we report that growth factors and cAMP act synergistically to stimulate proenkephalin gene expression. Positive synergy between growth factor- and cAMP-activated signaling pathways on gene expression has not been previously reported, and we suggest that these synergistic interactions represent a useful model for analyzing interactions between these pathways. Transfection and mutational studies indicate that both FGF-dependent gene activation and cAMP-dependent gene activation require cAMP response element 2 (CRE-2), a previously characterized cAMP-dependent regulatory element. Furthermore, multiple copies of this element are sufficient to confer FGF regulation upon a minimal promoter, indicating that FGF and cAMP signaling converge upon transcription factors acting at CRE-2. Among many different ATF/AP-1 factors tested, two factors, ATF-3 and c-Jun, stimulate proenkephalin transcription in an FGF- or Ras-dependent fashion. Finally, we show that ATF-3 and c-Jun form heterodimeric complexes in SK-N-MC cells and that the levels of both proteins are increased in response to FGF but not cAMP. Together, these results indicate that growth factor- and cAMP-dependent signaling pathways converge at CRE-2 to synergistically stimulate gene expression and that ATF-3 and c-Jun regulate proenkephalin transcription in response to both growth factor- and cAMP-dependent intracellular signaling pathways.
Creep and Corrosion Properties of the Extruded Magnesium Alloy Containing Rare Earth (2007)

Effects of microstructures on the creep and corrosion properties were investigated in the Mg-8Al alloys with addition of the rare earth elements (La-rich Mischmetal, RE). The addition of RE to Mg-8Al alloy may form a stable intermetallic phase, Al11RE3 at elevated temperature, and suppress the β phase (Mg17Al12) with poor stability at high temperature. The corrosion rate of the alloy slightly decreases with increasing the added RE contents. The constant-load creep behavior was examined at 423, 448 and 473 K under stresses between 40 and 100 MPa. The RE-containing alloy showed a prolonged period of stead-state creep compared to the Mg-8Al base alloy and slightly reduced minimum creep rates. A stress exponent of 2 estimated suggests that the creep behavior can be controlled by the grain boundary sliding. The apparent activation energy for creep of Mg-8Al and Mg-8Al-2RE alloys are 114 and 104 kJ mol−1, respectively.
Characterization of the novel duplicated PRLR gene at the late-feathering K locus in Lohmann chickens (2013)

A partial duplication of the prolactin (PRL) receptor gene (designated as dPRLR) has been identified at the late-feathering (LF) K locus on chromosome Z of some chicken strains recently, implying that dPRLR is probably a candidate gene associated with LF development in chickens. However, little is known about the structure, functionality, and spatiotemporal expression of the dPRLR gene in chickens. In this study, using 3'-RACE and RT-PCR, the full-length cDNA of the dPRLR obtained from the kidneys of male Lohmann layer chickens carrying a K allele was cloned. The cloned dPRLR is predicted to encode a membrane-spanning receptor of 683 amino acids, which is nearly identical to the original PRLR, except for its lack of a 149-amino acid C-terminal tail. Using a 5× STAT5-Luciferase reporter system and western blot analysis, we demonstrated that dPRLR expressed in HepG2 cells could be potently activated by chicken PRL and functionally coupled to the intracellular STAT5 signaling pathway, suggesting that dPRLR may function as a novel receptor for PRL. RT-PCR assays revealed that similar to the original PRLR gene, dPRLR mRNA is widely expressed in all embryonic and adult tissues examined including the skin of male Lohmann chickens with a K allele. These findings, together with the expression of PRL mRNA detected in the skin of embryos at embryonic day 20 and 1-week-old chicks, suggest that skin-expressed dPRLR and PRLR, together with plasma and skin-derived PRL, may be involved in the control of the LF development of chicks at hatching. Moreover, the wide tissue expression of dPRLR implies that dPRLR may regulate other physiological processes of chickens carrying the K allele.
Reprogramming wireless sensor networks with a selective and incremental approach (2008)

A wireless sensor network can be formed by nodes from different manufacturers, as long as their radio component enables communication with each other. Sometimes it is necessary to change the program in the nodes and several over-the-air reprogramming mechanisms have been proposed for that purpose. We base our work in one of such mechanisms, Deluge, adding some important contributions: we allow the selection of a set of nodes to be reprogrammed (not exclusively the whole network) and we allow incremental reprogramming (it is not mandatory to send complete program images). Through experiments with a real network we show that our proposal brings several benefits related to less traffic and less time spent in reprogramming tasks, which saves energy by using less the radio interface.
An economical large-scale procedure to purify Micrococcus plasmid DNA. (1990)

A reproducible and economical procedure for obtaining a large yield of highly purified covalently closed circular (ccc) plasmid DNA from an industrially important strain of Micrococcus is described. The procedure adopted here departs in several ways from commonly used protocols for isolation of plasmid DNA from Gram (positive) and Gram (negative) bacteria. The plasmid DNA prepared by this procedure is free of contaminants and is pure enough to be used for electron microscopy, DNA transformation, sequencing, in vitro transcription and mutagenesis.
Structural and functional characterization of the HPV16 E7 protein expressed in bacteria. (1993)

The E7 gene of the human papillomaviruses (HPV) encodes a 98-amino acid, multifunctional nuclear phosphoprotein with functional and structural similarities to adenovirus E1A and the papovavirus T antigens. E7 is a viral oncoprotein, which will cooperate with an activated ras oncogene to transform primary rodent cells, and can cooperate with the HPV E6 protein for the efficient immortalization of primary human keratinocytes. Due to the compelling epidemiological and experimental association between HPV infection and cervical cancer, we have undertaken a detailed study of the structure of the HPV16 E7 protein. The E7 protein was expressed in Escherichia coli as a native, unfused polypeptide, and soluble protein was purified by conventional chromatographic techniques. The purified protein was assessed for various biochemical and biophysical properties. Purified E7 binds the retinoblastoma protein avidly and specifically, and it can dissociate the E2F transcription factor when assayed in vitro. Circular dichroism spectroscopy indicated that E7 reversibly binds Zn2+ and Cd2+, resulting in a substantial increase in the alpha-helical content of the metal-bound E7 consistent with the stabilization of a hydrophobic core in the COOH terminus of the protein.
An Arthroscopic Technique for Anterior Stabilization of the Shoulder with a Bioabsorbable Tack* (1996)

Arthroscopically assisted repair of the anterior aspect of the labrum with use of a bioabsorbable tack was performed in fifty-two consecutive patients who had chronic anterior instability of the shoulder. The average age of the patients was twenty-eight years (range, sixteen to fifty years). The etiology of the instability was a traumatic injury in forty-nine patients; twenty-six of those injuries were sustained during participation in a contact sport. Fifty shoulders had a Bankart lesion. The patients were evaluated at an average of forty-two months (range, twenty-four to sixty months) after the procedure. Forty-one (79 per cent) of the patients were asymptomatic and were able to participate in sports without restriction. The repair was considered to have failed in eleven (21 per cent) of the patients. In four of them, the failure resulted from a single traumatic reinjury during participation in a contact sport, and three of these reinjuries were treated nonoperatively. The remaining seven failures occurred atraumatically. Eight patients had an open glenoid-based capsulorrhaphy as a consequence of recurrent instability. At the reoperation, no evidence of the tack was found in any patient. In seven patients, the Bankart lesion had completely healed, and the anteroinferior aspect of the capsule was patulous. Anterior stabilization of the shoulder with a bioabsorbable tack may be indicated for patients who have anterior instability but do not need a capsulorrhaphy or capsular imbrication to reduce the joint volume.
Heparin augments osteoclast resorption‐stimulating activity in serum (1991)

Increased numbers of mast cells are commonly seen at sites of increased bone resorption and in osteoporosis. Long-term administration of heparin, a major component of mast cell granules, causes osteoporosis. We therefore tested the effect of heparin on bone resorption by osteoclasts disaggregated from neonatal rat long bones. We found that, in the absence of serum, heparin was without effect on osteoclast function. However, in the presence of newborn calf serum, rat serum, or bovine platelet-poor plasma-derived serum, heparin, in the range 25-100 micrograms/ml, induced an increase in osteoclastic bone resorption. Heparin appeared to act through binding and enhancement of an osteoclast resorption-stimulating activity (ORSA) present in serum. A number of known factors that show an affinity for heparin, including transforming growth factor-beta, platelet-derived growth factors, insulin-like growth factors I or II, acidic or basic fibroblast growth factors, fibronectin, or laminin, could not substitute for ORSA, suggesting that the activity may represent a novel heparin-binding factor. The ability of glycosaminoglycans (GAGs) and related molecules to enhance resorption was dependent on the degree of sulfation and on their size: The high molecular weight GAG heparan sulfate and polysaccharides fucoidan or dextran sulfate showed a similar effect, while low molecular weight heparin, chondroitin-2-sulfate, chondroitin-4-sulfate, and chondroitin-6-sulfate were without effect. We propose that mast cells or heparin therapy increases bone resorption through augmentation of the activity of a factor involved in the local and systemic regulation of osteoclastic bone resorption.
프랑스 제3공화정 당시의 이념갈등과 사회통합 -뒤르케임의 공화주의 이념과 사회학의 역할을 중심으로 (2012)

이 논문은 프랑스 제 3공화정 시기에 활동했던 에밀 뒤르케임과 그 학파가 어떻게 그 당시의 이념갈등을 극복하고 사회통합을 성공적으로 끌어낼 수 있었는지, 공화주의 이념의 형성이라는 지성사적 맥락에 초점을 맞추어 연구한 것이다. 이 작업이 중요한 이유는 이 당시 새롭게 태어난 사회학이 그 학문적 정체성을 수립해가는 과정에서 현대 인문사회학자들이 주목해야 할 다음의 몇 가지 중요한 역할을 했기 때문이다. 첫째 다른 인문·사회과학들과의 협조를 통해서 사회현상을 통합적·학제적 시각에서 다루는 학문적 전통을 만들어냈다. 둘째 자유방임주의에 머물러 있던 19세기말 20세기 초 프랑스의 자유주의 이념을 적극적으로 확장해, 사회적 자유주의라는 이름으로 복지국가가 탄생하는 데 필요한 사회 철학적 이념을 제공하는 데 결정적 역할을 했다. 셋째, 자유방임주의와 사회주의라는 양극단의 사회발전 모델들 사이에서 공화주의라는 제 3의 발전모델을 제시함으로써, 제3공화정 초기 프랑스 사회의 양극화 현상과 극단적 이념의 혼란 상황을 수습하는데 결정적 기여를 했다. 이 작업을 통해 저자가 주장하는 바는 프랑스 제3공화정당시 뒤르케임과 그 학파의 탄생배경을 공화주의라는 이념모델로 접근할 때, 뒤르케임 이론의 핵심을 차지하는 주요개념들-유기적 연대, 도덕적 개인주의, 민주주의, 현대국가, 직업집단 이론이 유기적으로 더 잘 이해되고, 그의 사상 및 이론체계가 사회통합에 관심을 갖는 사회학도들에게 더욱 분명한 이론적·실천적 함의를 갖는 사회학적 교훈을 제공할 수 있다는 것이다.
FGD5 Mediates Proangiogenic Action of Vascular Endothelial Growth Factor in Human Vascular Endothelial Cells (2012)

This study provides the first demonstration of expression, subcellular localization, and function of FGD5 in vascular ECs. The results suggest that FGD5 regulates proangiogenic action of VEGF in vascular ECs, including network formation, permeability, directional movement, and proliferation.
Medullary arteries in aging and dementia. (1991)

We examined sclerotic changes of the medullary arteries in 110 nonneuropsychiatric patients ranging in age from the second to the ninth decades, in 20 patients with subcortical arteriosclerotic encephalopathy (Binswanger's disease), and in 20 patients with dementia of the Alzheimer type. The principal sclerotic change was fibrohyaline thickening of the wall, which began to appear during the late fourth decade, increased in incidence gradually with age, and was most severe in patients with subcortical arteriosclerotic encephalopathy. Morphometry showed that the sclerotic changes of the medullary arteries were most prominent in the frontal lobe, followed by the parietal, occipital, and temporal lobes, in both the nonneuropsychiatric and demented groups. The sclerotic rate in the frontal lobe of patients with dementia of the Alzheimer type was slightly higher than that in the nonneuropsychiatric patients (p less than 0.05) but far less than that in the patients with subcortical arteriosclerotic encephalopathy (p less than 0.001). The sclerotic rate correlated well with the degree of ischemic white matter changes as well as with blood pressure.
Crystal structure of selenosubtilisin at 2.0-.ANG. resolution (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCrystal structure of selenosubtilisin at 2.0-.ANG. resolutionRashid Syed, Zhen Ping Wu, James M. Hogle, and Donald HilvertCite this: Biochemistry 1993, 32, 24, 6157–6164Publication Date (Print):June 22, 1993Publication History Published online1 May 2002Published inissue 22 June 1993https://pubs.acs.org/doi/10.1021/bi00075a007https://doi.org/10.1021/bi00075a007research-articleACS PublicationsRequest reuse permissionsArticle Views281Altmetric-Citations64LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Management Information Systems and Student Affairs (1990)

Management Information Systems (MIS), although not clearly understood and defined in the student affairs discipline, is employed in student affairs offices throughout all colleges and universities. The benefits of using MIS to handle such tasks in student affairs as degree checking, classroom scheduling, transcript processing, and financial aid processing is now realized and appreciated by college or university administrators. This paper will briefly define MIS, and the impact of MIS on student affairs will be discussed. This paper also reviews past and present MIS applications, and suggests some possible future trends in the area of student affairs. Additionally, this paper examines the benefits and drawbacks of MIS applications in the student affairs area and outlines the techniques that can be employed to design and implement MIS in the student affairs environment.
New Absorption Liquids for the Removal of CO2 from Dilute Gas Streams using Membrane Contactors (2001)

Anthraquinone process, which is commonly used for production of hydrogen peroxide, includes an oxidation step where hydrogen peroxide is formed by the reaction between anthrahydroquinones and oxygen in an organic solvent.Oxidation is succeeded by extraction where hydrogen peroxide is extracted from the organic liquid by water and aqueous peroxide solution obtained.Oxidation is usually carried out in an empty or packed bubble column and extraction in a sieve-plate column.A new process was developed which allows the oxidation and extraction take place in the same space.The process is carried out in a tubular reactor where three phases are flowing concurrently.Both the equipment and plant size would be reduced considerably.The process has been tested in bench-scale but not in full production scale.
A Transcriptional Enhancer Required for the Differential Expression of the Human Estrogen Receptor in Breast Cancers (1997)

Breast cancers lacking estrogen receptor (ER) expression have an adverse prognosis and fail to respond to endocrine therapy. We have identified a transcriptional enhancer in the human ER gene which is differentially active in ER-positive (ER+) and ER-negative (ER-) human breast cancer cell lines. Enhancer function was mapped to a 35-bp element located from -3778 to -3744 upstream of the major human ER mRNA start site, which we have termed ER-EH0 (for estrogen receptor enhancer). Gel retardation assays with ER+ and ER- cell lines identified multiple DNA-protein complexes which specifically form on this enhancer. One of these complexes could be supershifted by anti-Jun or anti-Fos antibodies, identifying it as an AP-1-containing complex. Methylation interference assays suggest binding of factors to both the AP-1 site and adjacent base pairs. Enhancer activity requires both the AP-1 site and these adjacent sequences. Mutations introduced into ER-EH0 and the recently described proximal promoter element ERF-1 in the context of the full-length promoter confirm ER-EH0 as the dominant cis-acting element involved in differential ER expression.
In vitro Host Range of Equine Infectious Anemia Virus (1981)

Equine infectious anemia virus (EIAV) was successfully inoculated onto cell cultures of canine and feline origin, resulting in chronic infections in these cultures. Infection of equine cell cultures, which were the previous sole in vitro source demonstrated for virus production, was also performed for comparative purposes. Determination of the nature of the virus produced in the heterologous as well as the equine cells was accomplished in several ways. SDS-PAGE of purified virus from the different cell lines indicated very similar protein composition. Immunological identity was observed in gel diffusion tests employing an antiserum to the major core protein (p24) of equine-derived EIAV. Competition radioimmunoassays also indicated similar antigenicity in the viruses derived from the several cell lines. Strong relatedness was further demonstrated by hybridization of viral RNAs to EIAV complementary DNA. These data indicate that EIAV has an amphotropic cell culture host range and that the viruses isolated from the permissive lines were similar.
A New Approach for the Modification of Paper Surface Properties Using Polyoxometalates (2010)

A new approach for the chemical modification of the surface of paper based on the application of colloidal mixtures containing cationic starch and polyoxometalates on uncoated base paper is presented. Polyoxometalates with the Keggin-type structure and physical properties similar to those presented by coating pigments, namely H3PW12O40·23H2O, H4SiW12O40·24H2O, and K7PW11O39·9H2O, have been used in order to improve the quality of inkjet printing. The analysis of the different samples by FTIR-ATR spectroscopy showed the presence of the polyoxometalates (and the cationic starch) on the top surface of the paper. In addition, the determination of surface energy parameters, namely the polar component (σsp) and the dispersive component (σsd) of the surface energy, by contact angle measurements revealed that, for the new samples, the polar component level was much higher than that of the uncoated base paper. The quality of inkjet printing, evaluated by parameters such as the gamut area and the optical density, was considerably improved by these surface treatments.
Catalytic Asymmetric Synthesis of (<i>R</i>)-(-)-Calycotomine, (<i>S</i>)-(-)-Salsolidine and (<i>S</i>)-(-)-Carnegine (2006)

A simple and efficient procedure for a synthesis of isoquinoline alkaloids is described. The key step of the synthesis was a hydrocyanation of 6,7-dimethoxy-3,4-dihydroisoqunoline giving the corresponding 1-cyano-1,2,3,4-tetrahydroisoquinoline. The asymmetric Strecker reaction was accomplished in high yield and high enantiomeric excess using Jacobsen's thiourea-containing ­catalyst. The 1-cyanoisoquinoline thus obtained was transformed to natural products, (R)-(-)-calycotomine, (S)-(-)-salsolidine and (S)-(-)-carnegine.
Relationships between in vitro selenium supply, glutathione peroxidase activity, and phagocytic function in the HL-60 human myeloid cell line. (1985)

Utilizing the HL-60 human promyelocytic leukemia cell line cultured in defined medium, we examined the quantitative and temporal relationships between Se supply and the activity of the selenoenzyme glutathione peroxidase, as well as the effects of selenium deficiency on phagocytic function. Glutathione peroxidase activity depended on the medium Se concentration up to 2.6 X 10(-8) M (sodium selenate, 5 ng/ml), above which a plateau occurred. HL-60 cells grown in medium without Se supplementation became GSH peroxidase deficient, with activity 1-3% that of Se-replete cells. Replenishment of the medium with sodium selenate returned enzyme activity to 23% that of replete cells by 24 h and to 85% by 7 days, a process blocked by cycloheximide. Se-deficient HL-60 cells induced to granulocytic differentiation by dimethylformamide showed decreased hexose monophosphate shunt activity in response to phorbol myristate acetate and to an exogenous enzymatic H2O2-generating system. However, Se-deficient and -replete cells showed equal responses to methylene blue, which stimulates the shunt independently from the glutathione cycle. Se-deficient mature HL-60 cells stimulated with phorbol myristate acetate released 2.3-fold more H2O2 than Se-replete cells and only slightly (not significantly) less O2. Se-deficient and -replete differentiated HL-60 cells did not differ significantly in their capacities for cell motility or for ingestion of serum-opsonized bacteria. Differences between the findings of the present study and previous in vivo rat studies may reflect both the defined in vitro environment of the cell line and the inverse ratios of catalase and glutathione peroxidase activities in human and rat granulocytes.