gebhart/patrepeval huggingface dataset:
Brain serotonin turnover in morphine tolerant and dependent mice. (1970)

The rate of serotonin (5-HT) synthesis in the brains of mice rendered tolerant to and physically dependent on morphine either by s.c. implantation of a morphine pellet for three to four days or by daily s.c. injections of increasing doses of morphine was found to be about twice that of control animals receiving a placebo implant or daily saline injections. Inhibition of brain 5-HT synthesis by p -chlorophenylalanine was accompanied by a decrease in tolerance and dependence development to morphine. The concomitant administration of naloxone to reduce tolerance and physical dependence development to daily injections of morphine resulted also in a decrease in the rate of brain 5-HT synthesis. Although the roles of other biogenic amines have not been excluded, the results suggest that increased brain 5-HT synthesis may be associated with tolerance and physical dependence development to morphine in the mouse but do not establish a causal relationship.
A 3-D Sparse FIR Frustum Filter for Enhancing Broadband Plane Waves (2018)

A 3-D finite-extent impulse response (FIR) frustum filter having sparse coefficients is proposed for enhancing broadband plane waves (PWs) received by uniform aperture arrays. Motivated by the design technique of 1-D interpolated FIR filters, the proposed filter is realized by cascading a spatially-upsampled 3-D FIR prototype filter having a frustum-shaped passband and a 3-D FIR masking filter having a parallelepiped passband. Sparsity of the filter coefficients is achieved through the spatial upsampling of the coefficients of the prototype filter and by hard-thresholding the coefficients of both prototype and masking filters. The proposed 3-D FIR frustum filter saves 39.5% multiplications and 66.6% additions compared to the state-of-the-art 3-D FIR frustum filters without degrading the performance in enhancing broadband PWs.
Protein Phosphatase 2A Stabilizes Human Securin, Whose Phosphorylated Forms Are Degraded via the SCF Ubiquitin Ligase (2006)

Sister chromatid segregation is triggered at the metaphase-to-anaphase transition by the activation of the protease separase. For most of the cell cycle, separase activity is kept in check by its association with the inhibitory chaperone securin. Activation of separase occurs at anaphase onset, when securin is targeted for destruction by the anaphase-promoting complex or cyclosome E3 ubiquitin protein ligase. This results in the release of the cohesins from chromosomes, which in turn allows the segregation of sister chromatids to opposite spindle poles. Here we show that human securin (hSecurin) forms a complex with enzymatically active protein phosphatase 2A (PP2A) and that it is a substrate of the phosphatase, both in vitro and in vivo. Treatment of cells with okadaic acid, a potent inhibitor of PP2A, results in various hyperphosphorylated forms of hSecurin which are extremely unstable, due to the action of the Skp1/Cul1/F-box protein complex ubiquitin ligase. We propose that PP2A regulates hSecurin levels by counteracting its phosphorylation, which promotes its degradation. Misregulation of this process may lead to the formation of tumors, in which overproduction of hSecurin is often observed.
Synthesis and pharmacological screening of some N-carboxymethylbarbituric acid derivatives. I. (1979)

Reaction of haloacetate with barbital and methylphenobarbital in polar aprotic solvents in the presence of anhydrous K2CO3 gave N-carboalkoxymethyl derivatives in good yields. Acidic hydrolysis of the latter compounds affords N-carboxymethyl derivatives of barbital 8 or methylphenobarbital 7. From acid 7 and 8 via acid chlorides, amides 11--20 were prepared. These amides lack anticonvulsant activity and show only slight sedative and analgesic action.
Mitochondrial fission proteins in peripheral blood lymphocytes are potential biomarkers for Alzheimer’s disease (2012)

Altered mitochondrial fission proteins Drp1, SNO-Drp1, and Fis1 in PBL were relatively sensitive and specific in identifying AD patients and could be serving as a biomarker in the procedure of diagnosis.
Path Planning for Nonholonomic Multiple Mobile Robot System with Applications to Robotic Autonomous Luggage Trolley Collection at Airports (2020)

In this paper, we propose a novel path planning algorithm for the nonholonomic multiple mobile robot system with applications to a robotic autonomous luggage trolley collection system at airports. We consider this path planning algorithm as a Multiple Traveling Salesman Problem (MTSP). Our path planning algorithm consists of three parts. First, we use the Minimum Spanning Tree (MSP) algorithm to divide the MTSP into a number of independent TSPs, which achieves the task assignment for each mobile robot. Secondly, we implement a closed-loop forward control policy based on the kinematic model of the mobile robot to get a feasible and smooth path. The control cost of the path is used as the new metric in solving the TSPs. Finally, in order to adapt to our case, we modify the TSP as an Open Dynamic Traveling Salesman Problem with Fixed Start (ODTSP-FS) and implement an ant colony algorithm to achieve the path planning for each mobile robot. We evaluate our algorithm with simulation experiments and the experimental results demonstrate that our algorithm can quickly generate feasible and smooth paths for each robot while satisfying the nonholonomic constraints.
Bad Data Identification When Using Phasor Measurements (2007)

Synchronized phasor measurements obtained by phasor measurement units (PMU) are rapidly populating power systems. Among the expected benefits of having these measurements are the potential improvements in the accuracy and reliability of state estimation. On the other hand, state estimation formulation will have to be modified when phasor measurements are incorporated due to two issues. One is related to the reference bus selection which is no longer needed when phasor measurements are present. The other is the numerical problems encountered during initialization if current phasor measurements exist. This paper presents a reference-free rectangular state estimation method to address both of these problems. Furthermore, it is also shown that bad data in any of the phasor measurements can be detected and identified provided certain redundancy re quirements are met. These requirements are illustrated using example systems and measurement configurations.
Two-dimensional electrophoresis in basic and clinical research, as exemplified by studies on the exocrine pancreas. (1982)

Abstract I summarize our experience in the development and use of two-dimensional isoelectric focusing/SDS gel electrophoresis for the study of soluble proteins, in particular those of the exocrine pancreas. The importance of adding protease inhibitors to the first-dimension isoelectric focusing gel to prevent the autoactivation of potential pancreatic proteases and subsequent proteolysis is discussed. Recently developed methods for both the indirect and direct identification of protein spots by biological activity are summarized. I give examples of the importance of this technique in the study of basic mechanisms involved in the synthesis, segregation, and secretion of exportable proteins in the exocrine pancreas, as well as in the search for biochemical markers of pancreatic disease.
Construction, Expression, and Activities of L49-sFv-β-Lactamase, a Single-Chain Antibody Fusion Protein for Anticancer Prodrug Activation (1997)

The L49 (IgG1) monoclonal antibody binds to p97 (melanotransferrin), a tumor-selective antigen that is expressed on human melanomas and carcinomas. A recombinant fusion protein, L49-sFv-bL, that contains the antibody binding regions of L49 fused to the Enterobacter cloacae r2-1 beta-lactamase (bL) was constructed, expressed, and purified to homogeneity in an Escherichia coli soluble expression system. The variable regions of L49 were cloned by reverse transcription-polymerase chain reaction from L49 hybridoma mRNA using signal sequence and constant region primers. Construction of the gene encoding L49-sFv-bL was accomplished by hybridization insertion of VH, VL, and sFv linker sequences onto a pET phagemid template containing the bL gene fused to the pelB leader sequence. Optimal soluble expression of L49-sFv-bL in E. coli was found to take place at 23 degrees C with 50 microM isopropyl beta-D-thiogalactopyranoside induction and the use of the nonionic detergent Nonidet P-40 for isolation from the bacteria. Construction and expression of a soluble form of the p97 antigen in Chinese hamster ovary cells allowed affinity-based methods for analysis and purification of the fusion protein. Surface plasmon resonance, fluorescent activated cell sorting, and Michaelis-Menten kinetic analyses showed that L49-sFv-bL retained the antigen binding capability of monovalent L49 as well as the enzymatic activity of bL. In vitro experiments demonstrated that L49-sFv-bL bound to 3677 melanoma cells expressing the p97 antigen and effected the activation of 7-(4-carboxybutanamido)cephalosporin mustard (CCM), a cephalosporin nitrogen mustard prodrug. On the basis of these results, L49-sFv-bL was injected into nude mice with subcutaneous 3677 tumors, and localization was determined by measuring bL activity. Tumor to blood conjugate ratios of 13 and 150 were obtained 4 and 48 h post conjugate administration, respectively, and the tumor to liver, spleen, and kidney ratios were even higher. A chemically produced L49-Fab'-bL conjugate yielded a much lower tumor to blood ratio (5.6 at 72 h post administration) than L49-sFv-bL. Therapy experiments established that well-tolerated doses of L49-sFv-bL/CCM combinations resulted in cures of 3677 tumors in nude mice. The favorable pharmacokinetic properties of L49-sFv-bL allowed prodrug treatment to be initiated 12 h after the conjugate was administered. Thus, L49-sFv-bL appears to have promising characteristics for site-selective anticancer prodrug activation.
Enquête sur les archives industrielles rhodaniennes (1993)

Robert François. Enquête sur les archives industrielles rhodaniennes. In: Bulletin du Centre Pierre Léon d'histoire économique et sociale, 1993 n°1,1993. pp. 31-42.
Performance evaluation of hierarchical mass storage systems (1996)

One of the most interesting grand challenges that will drive high-performance computing and communications in the coming decade is the analysis of enormous amounts of satellite and simulation data to understand global effects such as weather front interactions and ocean-land-atmosphere interactions. These enormous amounts of data will impose tremendous demands on the underlying storage systems. To allow for the efficient storage and retrieval of such enormous amounts of data it is necessary to develop high-performance mass storage systems.
Mass storage systems consist of a hierarchy of storage media arranged in such an order as to provide a fast access, high capacity, low cost storage system. The continuous increase in user demands at scientific computing research centers for storage space forces system administrators to procure additional storage devices without access to tools for justifying such procurement. Furthermore, the plethora of available storage devices in the market with different performance characteristics makes this capacity planning process even more difficult.
This dissertation addresses this lack of tools for performance evaluation and capacity planning of hierarchical mass storage systems. Its main contributions are the development of queueing network models for both host attached and network attached device based mass storage systems. Two approximations were developed as components of this work. The first is an approximation of the performance of RAID devices in the setting of mass storage systems with multiple classes of requests. The second is an approximation of the simultaneous resource possession problem which occurs during tape to disk transfers in mass storage systems. The accuracy of both approximations was validated using process-based simulations. The third contribution of this dissertation is the development of Pythia--a tool for performance evaluation of hierarchical mass storage systems. Using the graphical user interface of Pythia, a user can easily describe the architecture of a mass storage system. The tool then automatically generates a queueing network model of the mass storage system and solves it using the modified multi-class approximate Mean Value Analysis algorithm with the approximations developed as part of this research.
Synthesis of mono-, oligo- and polyamide-cucurbituril rotaxanes (1998)

Mono-, oligo- and polyamide rotaxanes with the macrocycle cucurbituril are synthesized easily with high yields by interfacial condensation. The complex of cucurbituril with 1,6-hexanediammonium ion is used as preorganized structure. With different acid and diacid chlorides a great variety of amiderotaxanes and polyrotaxanes can be synthesized. The characterization is difficult due to the low solubility of cucurbituril and rotaxanes. But with the methods of IR, DTA, 1H NMR and elemental analyses, the existence of the rotaxanes is shown.
Inhibitors of the <i>C</i><sub>2</sub>-Symmetric HIV-1 Protease:  Nonsymmetric Binding of a Symmetric Cyclic Sulfamide with Ketoxime Groups in the P2/P2‘ Side Chains (1999)

Symmetric cyclic sulfamides, substituted in the P2/P2' position with functional groups foreseen to bind preferentially to the S2/S2' subsites of HIV-1 protease, have been prepared. Despite efforts to promote a symmetric binding, the sulfamides seemed prone to bind nonsymmetrically, as deduced from X-ray crystal structure analysis of one of the most potent inhibitors, possessing ketoxime groups in the P2/P2' side chains. Ab initio calculations suggested that the nonsymmetric conformation of the cyclic sulfamide scaffold had lower energy than the corresponding symmetric, cyclic urea-like conformation.
Analysis of Swath Bathymetry Sonar Accuracy (2005)

The practical limitations of many bottom mapping sonars lie in their ability to accurately estimate the angle of arrival. This paper addresses the accuracy of angle estimation when employed to determine the location of an extended target such as the bottom. A Gaussian model is assumed for the bottom backscatter and the corresponding Cramer-Rao lower bound for the variance of the angle estimate is determined for multi-element linear arrays. The paper focuses on determining the performance of high-resolution swath bathymetry sonars and, therefore, concentrates on the ability to determine bottom location with short pulses. Two error mechanisms, footprint shift and uncorrelated noise, are identified as important contributors to measurement errors. The two-element interferometric sonar configuration is investigated in detail. It is shown through the use of probability distributions, the Cramer-Rao bound, and simulation that it is difficult to get a good estimate of performance through simulation alone. Performance enhancement through pre-estimation and post-estimation averaging of multiple snapshots and changes in performance with pulse length and pulse rise time are also considered. Bottom estimation performance employing multi-element arrays is compared and contrasted with that of the two-element interferometric array. It is determined that there is little benefit associated with the multi-element array in terms of angle estimation performance alone. However, when other considerations such as angle ambiguities, multiple angles of arrival, and physical shortcomings associated with practical arrays are taken into account, the multi-element array is favored.
Rapid method for purification of soil DNA for hybridization and PCR analysis. (1993)

Monitoring of microbial DNA in soils by dot blot hybridization and PCR analysis is a useful technique for gaining insight into the survival and impact of genetically modified micro-organisms released in the environment. Most methods of DNA isolation from soils require a large number of purification steps rendering them unsuitable for quantitative analysis of multiple samples. Here we describe a very rapid method for the isolation and purification of multiple samples of soil DNA that can be used directly for dot blot hybridization and PCR analysis. Soil DNA extracts are prepared by lysozyme/SDS treatment at pH 9.0 and purified by ammonium acetate precipitation and Sephadex G50 gel filtration. In a practical application of this method, sandy soil samples were seeded with Alcaligenes eutrophus cells and exposed to high temperature (42 degrees C) or desiccation. As a result, the number of culturable A. eutrophus cells which could be recovered from the soil samples quickly declined. However, the concentration of a marker gene encoding resistance to cadmium, cobalt and zinc (czc) remained unaltered.
GEOMETRIC OPTIMIZATION OF PLANAR 3-<i>R<u>P</u>R</i> PARALLEL MECHANISMS (2007)

To pursue the maximal singularity-free workspace of parallel mechanisms is a very important concern for robot designers. This paper focuses on the case of planar 3-RPR parallel mechanisms. First, a relatively simple singularity equation of any point on the platform is derived. The obtained singularity equation shows that the singularity locus of any point on the platform is a circle of the same size, as long as the base and the platform are similar triangles. Furthermore, the three centres of the workspace circles lie exactly on the singularity circle. With these useful observations, the singularity-free workspace as well as the maximal leg length ranges can be determined. For a base of unit area, it is found that robots with equilateral triangle base and platform can obtain the maximal singularity-free workspace. Three case studies demonstrate this observation. Finally, a procedure for this kind of robot geometric design is provided.
Study of quadruped walking robot climbing and walking down slope (2002)

Presents an experimental study of climbing and walking down a slope by the JTUWM-II quadruped robot. A scheme by which the walking robot detects the slope and measures the gradient of the slope with tactile sensors is proposed. An algorithm for the body to turn parallel with the sloping surface is developed. In the transition area gait adjustment is made for stable walking. Also the maximum gradient of the slope for the robot and the optimum body height are given.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Production Process for High-Quality Pea-Protein Isolate with Low Content of Oligosaccharides and Phytate (2001)

A process for pea-protein isolate production, resulting in low content of phytate and oligosaccharides, has been developed. Oligosaccharides were removed from the protein fraction through ultrafiltration. Ultrafiltration of 50- and 100-kD molecular-weight cutoffs (MWCOs) were tested, and both effectively separated the oligosaccharides from the protein. Phytate degradation was achieved by incubation of the pea-protein solution by addition of exogenous phytase enzyme. An almost complete degradation of inositol hexa-, penta-, tetra-, and triphosphates was reached using an incubation time of 1 h. The reduced content of oligosaccharides and inositol phosphates is likely to result in reduced flatulence and improved mineral bioavailability. These qualities of the pea-protein isolate make it a suitable protein source for infant formula production.
A Predictive Approach Using Deep Feature Learning for Electronic Medical Records: A Comparative Study. (2018)

Massive amount of electronic medical records accumulating from patients and populations motivates clinicians and data scientists to collaborate for the advanced analytics to extract knowledge that is essential to address the extensive personalized insights needed for patients, clinicians, providers, scientists, and health policy makers. In this paper, we propose a new predictive approach based on feature representation using deep feature learning and word embedding techniques. Our method uses different deep architectures for feature representation in higher-level abstraction to obtain effective and more robust features from EMRs, and then build prediction models on the top of them. Our approach is particularly useful when the unlabeled data is abundant whereas labeled one is scarce. We investigate the performance of representation learning through a supervised approach. First, we apply our method on a small dataset related to a specific precision medicine problem, which focuses on prediction of left ventricular mass indexed to body surface area (LVMI) as an indicator of heart damage risk in a vulnerable demographic subgroup (African-Americans). Then we use two large datasets from eICU collaborative research database to predict the length of stay in Cardiac-ICU and Neuro-ICU based on high dimensional features. Finally we provide a comparative study and show that our predictive approach leads to better results in comparison with others.
The Skin Produces Urocortin<sup>1</sup> (2000)

Since the skin produces POMC peptides, in the present work we investigated local production of urocortin, a peptide related to CRH, the normal endogenous stimulant for POMC. Urocortin immunoreactivity was detected by direct RIA in extracts of human skin, mouse skin (C57BL-6 strain), cultured cells from established lines of human melanoma and squamous cell carcinoma, human keratinocytes (Ha-CaT), and hamster melanomas. Addition of a reverse phase high performance liquid chromatography step before the RIA confirmed the presence of urocortin, as the immunoreactivity eluted at the same retention time as urocortin standard in extracts from HaCaT keratinocytes and mouse skin. Using the tandem technique of liquid chromatography-mass spectrometry, we identified a peptide with the same mass and retention time as the urocortin standard in human skin extracts. The urocortin antigen could be immunolocalized to normal keratinocytes of the epidermis and hair follicle, epithelium of sweat and sebaceous glands, dermal skeletal muscle, and nevocytes; it was also detected in melanoma and basal cell carcinoma cells. RT-PCR amplification of ribonucleic acid from human skin, cultured keratinocytes, and melanoma cells showed a 145-kb fragment from the coding region of exon 2 of the urocortin gene in all of the tested sources. Lastly, sequencing of the amplified fragment confirmed 100% homology with the known sequence of the urocortin gene. In conclusion, we now demonstrate that human skin and mouse skin as well as cultured keratinocytes and melanoma cells exhibit functional expression of the urocortin gene with actual production of urocortin peptide.