gebhart/patrepeval huggingface dataset:
The structure and dynamics of the granulocyte macrophage colony-stimulating factor receptor defined by the ternary complex model (1993)

Myeloid cell lines and primary leukemic myeloblasts express two classes of granulocyte macrophage colony-stimulating factor (GM-CSF) binding sites of high (Kd 20-50 pM) and low affinity (Kd 5-10 nM). High affinity binding is caused by the association of two chains, p80 alpha and p130 beta, whereas p80 alpha alone confers low affinity binding only. Furthermore interleukin-3 (IL-3) competes for the binding of GM-CSF to its high affinity receptor (for review see Nicola, N. A., and Metcalf, D. (1991) Cell 67, 1-4). In the present study, we took advantage of the perturbation of GM-CSF binding equilibrium by IL-3 to take a quantitative approach to analysis of the structure and dynamics of the GM-CSF receptor complex. First, cross-linking studies were performed at two concentrations of radioligand. At 200 pM, a concentration sufficient for near saturation of the high affinity binding site R1, the association between p80 alpha and p130 beta is stoichiometric, and the addition of IL-3 prevents the binding to both chains. At 5 nM, a concentration sufficient for half-occupancy of the low affinity binding site R2, IL-3 prevents cross-linking to the beta chain only. Second, GM-CSF saturation curves were analyzed both at equilibrium and under conditions of perturbation of the equilibrium by IL-3. In the presence of IL-3, the interaction of GM-CSF with its receptor is converted from high to low affinity binding. Computer modeling of binding data with a ternary complex model involving GM-CSF, p80 alpha, and p130 beta indicates that the model fits the data with accuracy and suggests that ligand binding stabilizes the interaction between p80 alpha and p130 beta by 3 orders of magnitude. Third, membrane solubilization dissociates p80 alpha and p130 beta whereas on ligand-stabilized preformed complexes, solubilization did not dissociate the two chains. Finally, upon addition of GM-CSF, there is an increase with time in the proportion of ligand bound to the high affinity receptor, at the expense of that bound to low affinity receptor, suggesting that stabilization of the ternary complex is a time-dependent process.
<i>Aggregatibacter actinomycetemcomitans</i>biofilm killing by a targeted ciprofloxacin prodrug (2013)

A pH-sensitive ciprofloxacin prodrug was synthesized and targeted against biofilms of the periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). The dose required to reduce the viability of a mature biofilm of Aa by ~80% was in the range of ng cm(-2) of colonized area (mean biofilm density 2.33 × 10(9) cells cm(-2)). A mathematical model was formulated that predicts the temporal change in the concentration of ciprofloxacin in the Aa biofilm as the drug is released and diffuses into the bulk medium. The predictions of the model were consistent with the extent of killing obtained. The results demonstrate the feasibility of the strategy to induce mortality, and together with the mathematical model, provide the basis for design of targeted antimicrobial prodrugs for the topical treatment of oral infections such as periodontitis. The targeted prodrug approach offers the possibility of optimizing the dose of available antimicrobials in order to kill a chosen pathogen while leaving the commensal microbiota relatively undisturbed.
The mucopolysaccharidoses: a success of molecular medicine (2008)

The mucopolysaccharidoses represent a devastating group of lysosomal storage diseases affecting approximately 1 in 25 000 individuals. Advances in biochemistry and genetics over the past 25 years have resulted in the identification of the key hydrolases underlying the mucopolysaccharidoses, with subsequent isolation and characterisation of the genes involved. Ultimately these advances have led to the recent development of specific treatment regimens for some of the mucopolysaccharidoses, in the form of direct enzyme replacement. Direct replacement of the defective gene product has been attempted for very few genetic disorders, and thus the experience gained in the lysosomal storage diseases by the development, evaluation and integration of treatment regimens into healthcare is instructive for other rare genetic disorders. This review focuses on the pathophysiology of the mucopolysaccharidoses and highlights the complex biochemical and physiological perturbations that underlie the disease phenotype.
Impregnation and Drying (2009)

This chapter contains sections titled: Introduction Impregnation Drying The Chemistry Impregnation and Drying of an MoOx/Al2O3 Catalyst Conclusions References
Can oxytocin treat autism? (2015)

We are still at an early stage of assessing oxytocin-based therapy for autism spectrum disorders
Dengue Virus Infection of B Lymphocytes In Vitro. (2012)

Dengue virus infection of certain subsets of the immune system, especially dendritic cells and macrophages, is well-established in the literature. On these cells, the virus can use DC-SIGN as a receptor. Infection of B lymphocytes is debated, and since B cells upregulate DC-SIGN upon activation, we hypothesize that this receptor may also be used for DENV infection of B cells. In this thesis, I show that DENV-2 infects primary aB and does not infect resting B cells, while DENV-1 infects neither resting nor aB. DENV-2 infection of aB is DC-SIGN dependent. Infection of Raji/DC-SIGN cells with DENV-2 is DC-SIGN dependent, while Raji/0 infection is observed. Infection is detected via flow cytometry using DENV-specific antibodies: 2H2 for DENV-2 infection, and 3H5.1 for DENV-1 infection. Statement of Public Health Impact: Dengue virus infection incidence has increased in recent decades, and poses a particular burden on children in South and Central America. It is known that cells of the immune system are directly involved with disease pathogenesis, and so further investigating infection of specific immune cells is crucial to understanding how best to prevent negative outcomes of DENV infection. B lymphocytes are a key part of the adaptive immune system, and may be infected by DENV. My thesis directly investigates this question, and also examines if the C-type lectin DC-SIGN is the receptor used by the virus for infection.
The use of <i>N</i>-methylprotoporphyrin dimethyl ester to inhibit ferrochelatase in <i>Rhodopseudomonas sphaeroides</i> and its effect in promoting biosynthesis of magnesium tetrapyrroles (1982)

N-Methylprotoporphyrin dimethyl ester inhibits ferrochelatase in isolated membranes of Rhodopseudomonas sphaeroides at low concentrations (around 10 nm). Full inhibition developed after a short lag phase. The inhibition was non-competitive with porphyrin substrate. Addition of inhibitor to growing cultures of Rps. sphaeroides caused a decrease (near 40%) in cytochrome content and a severe inhibition of ferrochelatase; the excretion of haem into the medium by cell suspensions was also severely inhibited. The addition of N-methylprotoporphyrin dimethyl ester to suspensions of photosynthetically competent Rps. sphaeroides Ga caused excretion of Mg-protoporphyrin monomethyl ester. When added to mutants V3 and O1, magnesium divinylphaeoporphyrin a5 monomethyl ester and 2-devinyl-2-hydroxyethylphaeophorbide a were excreted, with maximum effect at around 3 microM-inhibitor in the medium. The results are interpreted to suggest that the inhibitor decreases concentration of intracellular haem, which normally controls the activity of 5-aminolaevulinate synthetase. Unregulated activity of this enzyme leads to overproduction of protoporphyrin, which is diverted to the bacteriochlorophyll pathway. Further control operates at magnesium protoporphyrin ester conversion in normal cells.
Subthreshold Swing Reduction by Double Exponential Control Mechanism in an MOS Gated-MIS Tunnel Transistor (2015)

The current of an MIS tunnel diode was tremendously changed (about five orders) while sweeping the gate bias of an MOS capacitor nearby. It was supposed that the effective Schottky barrier height of the MIS tunnel diode was affected by the bias of the nearby MOS gate capacitor via changing the minority carrier distribution at the fringe of the MIS tunnel diode. Therefore, the subthreshold behavior of the MIS tunnel diode current was controlled by the voltage bias of the nearby MOS capacitor, which is called MOS gated-MIS tunnel transistor. The minimum subthreshold swing of the MOS gated-MIS tunnel transistor could reach 15.3 mV/decade empirically.
99mTc-Tilmanocept: A Novel Molecular Agent for Lymphatic Mapping and Sentinel Lymph Node Localization (2015)

Preoperative lymphatic mapping in conjunction with intraoperative γ-probe detection is widely used for sentinel node localization in melanoma, breast cancer, and other malignancies. (99m)Tc-radiocolloids have been the standard radiotracers used for sentinel node mapping. (99m)Tc-tilmanocept is a receptor-binding molecular imaging agent approved by the U.S. Food and Drug Administration for lymphatic mapping and lymph node localization in breast cancer, melanoma, clinically node-negative squamous cell carcinoma of the oral cavity, and other solid tumors. It has several advantages over conventional radiocolloids, including rapid injection site clearance, high sentinel node extraction, and low distal node accumulation, which can lead to efficient resource use.
Electrospinning (2012)

INTRODUCTION Fibers - Key Functional Elements in Technology and Nature Some Background Information Processing of Polymer Materials towards Fibers - Fiber Extrusion Routes to More Advanced Fibers - Mimicking Nature Electrospinning Electrospinning - Important Facts to Remember NATURE OF THE ELECTROSPINNING PROCESS - EXPERIMENTAL OBSERVATIONS AND THEORETICAL ANALYSIS Experimental Setups Experimental Observations on Fiber Formation Theoretical Analysis of the Nature of the Electrospinning Processes Nature of the Electrospinning Process - Important Facts NANOFIBER PROPERTIES Parameters Controlling Nanofiber Formation Short Account on Methods of Analysis for the Structure of Electrospun Nanofibers Control of Nanofiber Diameters Shape of the Fibers Nanofiber Topologies, Porous Fibers Nanofiber Trajectories in the Deposition Plane Internal Morphology of Electrospun Nanofibers Mechanical Properties of Single Nanofibers Nanofiber Properties - Important Facts to Remember NONWOVENS COMPOSED OF ELECTROSPUN NANOFIBERS Nanofiber Nonwovens - Functional Elements for Technical Applications Methods of Analysis for Properties on Nonwovens Fiber Arrangements in Nonwovens Heterogeneous Nonwovens Porosity and Pore Structures - Theoretical Modeling and Experimental Analysis Mechanical Properties of Nonwovens Nonwovens Composed of Electrospun Nanofibers - Important Facts to Remember ELECTROSPINNING - SOME TECHNICAL ASPECTS Technical Setups for Creating Jets Designs of Counterelectrode Configurations Allowing Preparation of Nonwovens with a Broad Range of Architecture Electrospinning - Some Technical Aspects - Important Facts to Remember MODIFICATION OF THE ELECTROSPINNING TECHNIQUE Towards Advanced Modes of Fiber Preparation and Deposition Near-Field Electrospinning - High-Precision Deposition Electrospinning Towards Core-Shell and Hollow Fibers Modification of the Electrospinning Technique - Important Facts to Remember MATERIALS CONSIDERATIONS Introduction Spinning from Organic Solvents Spinning of Water-Soluble Polymers Spinning of Biopolymers Spinning of Complex Polymer Systems Nanofibers from Polymer Hybrids, Metals, Oxides Melt Spinning Materials Considerations - Important Facts to Remember TECHNICAL APPLICATIONS OF ELECTROSPUN NANOFIBERS Nanofibers, Nanofiber Nonwovens - Elements of Nanotechnology Filter Applications Textile Applications Catalysis Nanofiber Reinforcement Surface Modifications Template Applications Plant Protection via Pheromones Technical Applications - Important Facts to Remember MEDICINAL APPLICATIONS FOR ELECTROSPUN NANOFIBERS Nanotechnology and Medicinal Applications in General Tissue Engineering Wound Healing Transport and Release of Drugs Nanotechnology and Medicinal Applications in General - Important Facts to Remember
Cardiomyoblast-like Cells Differentiated from Human Adipose Tissue-Derived Mesenchymal Stem Cells Improve Left Ventricular Dysfunction and Survival in a Rat Myocardial Infarction Model (2009)

Adipose tissue-derived mesenchymal stem cells (ADMSCs) are multipotent cells. Here we examined whether human ADMSCs (hADMSCs) could differentiate into cardiomyoblast-like cells (CLCs) by induction with dimethylsulfoxide and whether the cells would be utilized to treat cardiac dysfunction. Dimethylsulfoxide induced the expression of various cardiac markers in hADMSCs, such as alpha-cardiac actin, cardiac myosin light chain, and myosin heavy chain; none of which were detected in noncommitted hADMSCs. The induced cells were thus designated as hADMSC-derived CLCs (hCLCs). To confirm their beneficial effect on cardiac function, hCLC patches were transplanted onto the Nude rat myocardial infarction model, and compared with noncommitted hADMSC patch transplants and sham operations. Echocardiography demonstrated significant short-term improvement of cardiac function in both the patch-transplanted groups. However, long-term follow-up showed rescue and maintenance of cardiac function in the hCLC patch-transplanted group only, but not in the noncommitted hADMSC patch-transplanted animals. The hCLCs, but not the hADMSCs, engrafted into the scarred myocardium and differentiated into human cardiac troponin I-positive cells, and thus regarded as cardiomyocytes. Transplantation of the hCLC patches also resulted in recovery of cardiac function and improvement of long-term survival rate. Thus, transplantation of hCLC patches is a potentially effective therapeutic strategy for future cardiac tissue regeneration.
La sociedad entre la universidad e institución de salud y su importancia en la formación de los alumnos de grado de psicología (2012)

This study intends to investigate the contributions that the partnership University/Health Service provides to undergraduate students, especially in hospitals. The research was conducted through semi-directed interviews with ten internship coordinators from ten different hospitals and twenty senior Psychology students. The survey results show that the partnership is highly valued and motivated by the perspective that the association benefits and helps both sides, which can be determined by the resonance of predominantly positive aspects in the speech of the interviewees. However, the negative aspects of the partnership were relativized denoting a certain resistance in face of questions and possible changes, not favoring a space for reflection able to lead to important advances in the establishment of new health practices in order to satisfactorily meet the real needs and demands of the population. Keywords: Health Psychology, Partnership, University, Health Services, Professional Training.
Reversible phase transfer and fractionation of Au nanoparticles by pH change (2010)

The reversible transfer of Au nanoparticles between water and chloroform phases by pH change was achieved by using a long-chain amine derivative. Further, we demonstrated that the phase transfer method was very effective for the fractionation of Au nanoparticles according to their crystal facet distinction.
Effects of Thickness on Thermal Stresses in a Thin Solder or Adhesive Layer (1992)

Research Papers Effects of Thickness on Thermal Stresses in a Thin Solder or Adhesive Layer An-Yu Kuo, An-Yu Kuo Structural Integrity Associates, Inc., San Jose, CA 95118 Search for other works by this author on: This Site PubMed Google Scholar Kuan-Luen Chen Kuan-Luen Chen Structural Integrity Associates, Inc., San Jose, CA 95118 Search for other works by this author on: This Site PubMed Google Scholar Author and Article Information An-Yu Kuo Structural Integrity Associates, Inc., San Jose, CA 95118 Kuan-Luen Chen Structural Integrity Associates, Inc., San Jose, CA 95118 J. Electron. Packag. Jun 1992, 114(2): 199-202 (4 pages) https://doi.org/10.1115/1.2906418 Published Online: June 1, 1992 Article history Received: August 1, 1991 Online: April 28, 2008
Kinetic and optical relaxation studies of adsorbed intermediates in some electrochemical reactions (1973)

In certain electrocatalytic reactions, co-adsorption of more than one electroactive intermediate may arise. The charge under the overall potentiodynamic current-potential profile for the adsorbed species may not then be used to evaluate coverage by the species. A method is proposed for resolution of processes involving more than one electrochemisorbed species when the electroactivity of, e.g., two species arises over a common potential range. The method is based on the different kinetic relaxation response of “reversibly” electrosorbed species from that for more “irreversibly” electrosorbed species. Applications to the case of reduction of adsorbed nitriles at Pt enables a two-stage mechanism to be formulated and in the reduction of benzene the charges for co-adsorption of H and benzene reduction can be distinguished. In the initial stages of Pt surface oxidation, the method enables the reversibly electrosorbed OH species to be distinguished from more irreversibly reactive rearranged surface oxide.The behaviour of chemisorbed CH3CN and the oxide film on Pt below a monolayer have also been investigated by a.c. modulated reflectance over a range of a.c. frequencies and wavelengths of light.
Knowledge-guided automatic thresholding for 3-dimensional display of head MRI images (2003)

A segmentor is described which automatically extracts soft tissues from the multisliced magnetic resonance imaging (MRI) of the head region by an iterative thresholding procedure. 3-D graphic displays are shown of the segmented soft tissues. To get the optimum threshold value, a goodness measure is used based on the knowledge of the object.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A New Class of <i>Caulobacter crescentus</i> Flagellar Genes (1998)

ABSTRACT Eight Caulobacter crescentus flagellar genes, flmA , flmB , flmC , flmD , flmE , flmF , flmG , and flmH , have been cloned and characterized. These eight genes are clustered in pairs ( flmAB , flmCD , flmEF , and flmGH ) that appear to be structurally organized as operons. Homology comparisons suggest that the proteins encoded by the flm genes may be involved in posttranslational modification of flagellins or proteins that interact with flagellin monomers prior to their assembly into a flagellar filament. Expression of the flmAB , flmEF , and flmGH operons was shown to occur primarily in predivisional cells. In contrast, the flmCD operon was expressed throughout the cell cycle, with only a twofold increase in predivisional cells. The expression of the three temporally regulated operons was subject to positive regulation by the CtrA response regulator protein. Mutations in class II and III flagellar genes had no significant effect on the expression of the flm genes. Furthermore, the flm genes did not affect the expression of class II or class III flagellar genes. However, mutations in the flm genes did result in reduced synthesis of the class IV flagellin proteins. Taken together, these data indicate that the flm operons belong to a new class of flagellar genes.
Structure and Electrochemical Properties of Li1 − x  ( Ni y Co1 − y  ) 1 + x  O 2 : Effect of Chemical Delithiation at 0°C (1995)

Acid treatment at 0°C of solid solutions yields delithiated well crystalline oxides which do not contain protons. For the nearly stoichiometric nickel‐rich oxides, removal of more than 20% of the lithium leads to a monoclinic distortion of the rhombohedral unit cell. Electron paramagnetic resonance (EPR) of low‐spin Ni3+ ions shows that for up to 0.2 extracted lithium, the generated Ni4+ ions are statistically distributed in the layers while above 0.2 extracted lithium the Ni4+ ions trend to order. During acid delithiation the oxidation of Ni3+ to Ni4+ ions proceeds prior to the oxidation of Co3+ to Co4+ as for a nonaqueous electrolyte. Discharge‐charge cycles of lithium cells using acid‐delithiated oxides as cathode materials show a significant improvement of reversibility compared with the charge‐discharge cycles of the corresponding pristine samples.
The physical and mechanical properties of hafnium orthosilicate: experiments and first-principles calculations (2020)

Hafnium orthosilicate (HfSiO4: hafnon) has been proposed as an environmental barrier coating (EBC) material to protect silicon coated, silicon-based ceramic materials at high temperatures and as a candidate dielectric material in microelectronic devices. It can naturally form at the interface between silicon dioxide (SiO2) and hafnia (HfO2). When used in these applications, its coefficient of thermal expansion (CTE) should match that of silicon and SiC composites to reduce the stored elastic strain energy, and thus risk of failure of these systems. The physical, mechanical, thermodynamic and thermal transport properties of hafnon have been investigated using a combination of both density functional theory (DFT) calculations and experimental assessments. The average linear coefficient of thermal expansion (CTE) calculated using the quasi-harmonic approximation increase from 3.06 10-6 K-1 to 6.36 10-6 K-1, as the temperature increases from 300 to 1500 K, in agreement with both X-ray diffraction lattice parameter and dilatometry measurements. The predicted thermal conductivity from Boltzmann transport theory was approximately 18 W/m.K at 300K. Both hot disk and laser flash measurements gave a thermal conductivity of 13.3 W/m.K. This slightly lower value is indicative of residual disorder in the experimental samples that was absent in the theoretical analysis. First-principles calculations and nanoindentation techniques were used to assess the ambient temperature elastic constants and bulk modulus respectively. The elastic properties obtained by both approaches agreed to within 5% validating the computational approach and its future use for study of the thermomechanical properties of other oxides or silicates.
A Self-Biased Operational Transconductance Amplifier in 0.18 micron 3D SOI-CMOS (2007)

We report on the design fabrication and testing of a wide range transconductance amplifier fabricated in the 0.18 <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">μ</i> m MIT Lincoln Labs 3D SOI-CMOS process. The amplifier is designed to operate in subthreshold and employs self-biased cascode transistors to minimize the bias lines transversing the 3 tiers in the technology.