gebhart/patrepeval huggingface dataset:
Correct memory operation of cache-based multiprocessors (1987)

This paper shows that cache coherence protocols can implement indivisible synchronization primitives reliably and can also enforce sequential consistency. Sequential consistency provides a commonly accepted model of behavior of multiprocessors. We derive a simple set of conditions needed to enforce sequential consistency in multiprocessors. These conditions are easily applied to prove the correctness of existing cache coherence protocols that rely on one or multiple broadcast buses to enforce atomicity of updates; in these protocols, all processing elements must be connected to the broadcast buses. The conditions are also used in this paper to establish new protocols which do not rely on the atomicity of updates and therefore do not require single access buses to propagate invalidations or to perform distributed WRITEs. It is also shown how such protocols can implement atomic READ&MODIFY operations for synchronization purposes.
Pharmacokinetics-Pharmacodynamics of a Novel β-Lactamase Inhibitor, CB-618, in Combination with Meropenem in an <i>In Vitro</i> Infection Model (2016)

The usefulness of β-lactam antimicrobial agents is threatened as never before by β-lactamase-producing bacteria. For this reason, there has been renewed interest in the development of broad-spectrum β-lactamase inhibitors. Herein we describe the results of dose fractionation and dose-ranging studies carried out using a one-compartment in vitro infection model to determine the exposure measure for CB-618, a novel β-lactamase inhibitor, most predictive of the efficacy when given in combination with meropenem. The challenge panel included Enterobacteriaceae clinical isolates, which collectively produced a wide range of β-lactamase enzymes (KPC-2, KPC-3, FOX-5, OXA-48, SHV-11, SHV-27, and TEM-1). Human concentration-time profiles were simulated for each drug, and samples were collected for drug concentration and bacterial density determinations. Using data from dose fractionation studies and a challenge Klebsiella pneumoniae isolate (CB-618-potentiated meropenem MIC = 1 mg/liter), relationships between change from baseline in log10 CFU/ml at 24 h and each of CB-618 area under the concentration-time curve over 24 h (AUC0-24), maximum concentration (Cmax), and percentage of the dosing interval that CB-618 concentrations remained above a given threshold were evaluated in combination with meropenem at 2 g every 8 h (q8h). The exposure measures most closely associated with CB-618 efficacy in combination with meropenem were the CB-618 AUC0-24 (r(2) = 0.835) and Cmax (r(2) = 0.826). Using the CB-618 AUC0-24 indexed to the CB-618-potentiated meropenem MIC value, the relationship between change from baseline in log10 CFU/ml at 24 h and CB-618 AUC0-24/MIC ratio in combination with meropenem was evaluated using the pooled data from five challenge isolates; the CB-618 AUC0-24/MIC ratio associated with net bacterial stasis and the 1- and 2-log10 CFU/ml reductions from baseline at 24 h were 27.3, 86.1, and 444.8, respectively. These data provide a pharmacokinetics-pharmacodynamics (PK-PD) basis for evaluating potential CB-618 dosing regimens in combination with meropenem in future studies.
A new serine protease gene from Trichoderma harzianum is expressed in Saccharomyces cerevisiae (2009)

Serine proteases are highly conserved among fungi and considered to play a key role in different aspects of fungal biology. These proteases can be involved in development and have been related to biocontrol processes. Difficulties in heterologous expression in a bacterial or yeast host have hampered engineering of these proteases for industrial application. We report here a successful expression of the serine protease SL41 from a biocontrol fungus Trichoderma harzianum in Saccharomyces cerevisiae. A new serine proteases gene SL41 has been cloned from T. harzianum. The full length cDNA was isolated by 5' and 3'rapid amplification of cDNA ends. The isolated cDNA of SL41 was then sequenced. The results showed that the open reading frame of SL41 was 1.617 bp long, encoding 538 amino acids. The cloning vector pMD18-T and an E. coli DH5alpha host were used to yield clones as E. coli DH5alpha/SL41. The SL41 gene was integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/SL41. Serine protease expressed by pYES2/SL41 was induced by galactose (maximal activity 16.5 units ml(-1) at 40 degrees C, pH 8.0) and was produced in fermentation liquid cultured for 60 h. Northern blot analysis indicated that SL41 transcripts are differentially accumulated at different culture time.
Formation mechanism of dip peaks in nonsuppressed ion chromatography (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFormation mechanism of dip peaks in nonsuppressed ion chromatographyTetsuo. Okada and Tooru. KuwamotoCite this: Anal. Chem. 1984, 56, 12, 2073–2078Publication Date (Print):October 1, 1984Publication History Published online1 May 2002Published inissue 1 October 1984https://pubs.acs.org/doi/10.1021/ac00276a022https://doi.org/10.1021/ac00276a022research-articleACS PublicationsRequest reuse permissionsArticle Views120Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Angiotensin-(1-7) and Nitric Oxide Interaction in Renovascular Hypertension (1995)

New studies suggest that vasodilator systems may play an important role in restraining the rise in peripheral vascular resistance associated with the evolution of arterial hypertension. We characterized in conscious dogs the hemodynamic and hormonal effects of 4 weeks of feeding either the nitric oxide synthase inhibitor N omega-nitro-L-arginine (3 mg.kg-1.d-1) or the nitric oxide precursor L-arginine (0.3 mg.kg-1.d-1) during the evolution of two-kidney, one clip hypertension. Inhibition of nitric oxide production elicited a form of hypertension more severe than that produced in placebo-fed two-kidney, one clip dogs. The higher levels of blood pressure were accompanied by lower levels of plasma renin activity and lower angiotensin II concentrations. During the chronic phase of renovascular hypertension, the fall in blood pressure produced by acute systemic injections of lisinopril or losartan was significantly reduced in dogs given the nitric oxide inhibitor. In contrast, chronic administration of L-arginine had no effect on the magnitude of hypertension or on the increases in renin activity and hyperangiotensinemia associated with the evolution of renal hypertension. Likewise, the fall in blood pressure produced by pharmacological blockade of angiotensin II was not different from that recorded in untreated renal hypertensive dogs. The vasodilator component of the blood pressure response due to intravenous injections of angiotensin-(1-7) (1 to 100 nmol/kg) was augmented in both untreated and L-arginine-treated two-kidney, one clip hypertensive dogs, but was significantly attenuated in hypertensive dogs fed the nitric oxide synthase inhibitor.(ABSTRACT TRUNCATED AT 250 WORDS)
The TAR RNA-binding Protein, TRBP, Stimulates the Expression of TAR-containing RNAs in Vitro and in VivoIndependently of Its Ability to Inhibit the dsRNA-dependent Kinase PKR (2003)

TRBP (HIV-1 transactivating response (TAR) RNA-binding protein) and PKR, the interferon-induced dsRNA-regulated protein kinase, contain two dsRNA binding domains. They both bind to HIV-1 TAR RNAs through different sites. Binding to dsRNA activates PKR that phosphorylates the eukaryotic initiation factor eIF-2alpha leading to protein synthesis inhibition. TRBP and PKR can heterodimerize, which inhibits the kinase function of PKR and has a positive effect on HIV-1 expression. In this study, an in vitro reticulocyte assay revealed the poor expression of TAR containing CAT RNAs compared with CAT RNAs. Addition of TRBP restored translation efficiency of TAR-CAT RNA and decreased the phosphorylation status of eIF-2alpha, confirming its role as a PKR inhibitor. Unexpectedly, eIF-2alpha was phosphorylated in the presence of TAR-CAT as well as CAT RNA devoid of the TAR structure. TRBP inhibited eIF-2alpha phosphorylation in both cases, suggesting that it restores the translation of TAR-CAT RNA independently and in addition to its ability to inhibit PKR. TRBP activity on gene expression was then analyzed in a PKR-free environment using PKR-deficient murine embryo fibroblasts. In a transient reporter gene assay, TRBP stimulated the expression of a TAR-containing luciferase 3.8-fold whereas the reporter gene with mutated TAR structures or devoid of TAR was stimulated 1.5- to 2.4-fold. Overall, the activity of TRBP2 was higher when the 5'-end of the mRNA was structured and was mediated independently by each dsRBD in TRBP. Increasing concentrations of TRBP showed no significant modification of the luciferase RNA levels, suggesting that TRBP stimulates translation of TAR-containing RNAs. Therefore, TRBP is an important cellular factor for efficient translation of dsRNA containing transcripts, both by inhibiting PKR and in a PKR-independent pathway.
Homogeneous Catalysis for Sustainable Hydrogen Storage in Formic Acid and Alcohols (2017)

Hydrogen gas is a storable form of chemical energy that could complement intermittent renewable energy conversion. One of the main disadvantages of hydrogen gas arises from its low density, and therefore, efficient handling and storage methods are key factors that need to be addressed to realize a hydrogen-based economy. Storage systems based on liquids, in particular, formic acid and alcohols, are highly attractive hydrogen carriers as they can be made from CO<sub>2</sub> or other renewable materials, they can be used in stationary power storage units such as hydrogen filling stations, and they can be used directly as transportation fuels. However, to bring about a paradigm change in our energy infrastructure, efficient catalytic processes that release the hydrogen from these molecules, as well as catalysts that regenerate these molecules from CO<sub>2</sub> and hydrogen, are required. In this review, we describe the considerable progress that has been made in homogeneous catalysis for these critical reactions, namely, the hydrogenation of CO<sub>2</sub> to formic acid and methanol and the reverse dehydrogenation reactions. The dehydrogenation of higher alcohols available from renewable feedstocks is also described. Key structural features of the catalysts are analyzed, as is the role of additives, which are required in many systems. Particular attention is paid to advances in sustainable catalytic processes, especially to additive-free processes and catalysts based on Earth-abundant metal ions. Mechanistic information is also presented, and it is hoped that this review not only provides an account of the state of the art in the field but also offers insights into how superior catalytic systems can be obtained in the future.
Recent Developments in Studies of l-Stepholidine and its Analogs: Chemistry, Pharmacology and Clinical Implications (2007)

Tetrahydroprotoberberines (THPBs) represent a series of compounds extracted from the Chinese herb Corydalis ambigua and various species of Stephania. THPBs, dependent on the presence of hydroxyl groups in its structure, are divided into three types: nonhydroxyl-THPBs, monohydroxyl-THPBs and dihydroxyl-THPBs. THPBs are identified as a new category of dopamine receptor ligands. Among all THPBs, dihydroxyl-THPBs attracted particular attention because of their dual actions on dopamine (DA) receptors. They exhibit D(1) receptor agonistic activity while acting as D(2) receptor antagonists. This unique pharmacological profile made dihydroxyl-THPBs such as l-stepholidine (l-SPD) potential agents in the treatment of drug addiction, Parkinson's disease, and especially, schizophrenia. Clinical studies have shown that co-administration of l-SPD with a typical antipsychotic drug significantly enhances the therapeutic effects and remarkably reduces the tardive dyskinesia induced by the typical antipsychotic drug used with schizophrenic patients. Moreover, l-SPD alone was shown to have therapeutic value without inducing significant extrapyramidal side effects and also seemed to reduce the negative symptoms of schizophrenia. This is confirmed in experimental studies using animal models of schizophrenia, in which l-SPD improved social interaction and cognitive function, inhibited hyperactivity in schizophrenic animals. This review discusses the chemistry, pharmacology and clinical implications of l-THPBs in the drug development for psychosis and neurobiological diseases.
Development of Shape Memory Alloy (SMA) Actuated Mechanisms for Spacecraft Release Applications (1999)

Current methods of deploying spacecraft payloads typically employ explosive-type separation devices which do not address shock concerns and are not resettable without disassembly from the spacecraft or significant refurbishment of parts. Other separation mechanisms exist which eliminate explosives, but also do not offer reduced shock or the advantages of insitu resettability. Several new, shape-memory alloy (SMA) actuated release mechanisms have been developed for satellite release applications for loads of 500 lbf and greater. These mechanisms all offer fast, non-pyro releases and are completely testable and reusable while mounted on the spacecraft. The Qwknut and the Low-Force Nut (LFN) are suitable for release applications up to 3000 lbf, while the Fast-Acting, Shockless Separation Nut (FASSN) is suitable for higher load releases above 5000 lbf. FASSNs have been successfully prototyped for release applications of 80,000 lbf. Shawn H. Smith Applications for SMA mechanisms include satellite hold-down and release from the launch vehicle, hold-down and release of solar panels, and hold-down and release of cover panels and satellite appendages.
The Influence of Fuel Composition on Emissions of Carbon Monoxide and Oxides of Nitrogen (1970)

<div class="htmlview paragraph">A theoretical and experimental investigation has been carried out in an attempt to determine the effect of fuel composition on the exhaust emission levels of carbon monoxide and nitric oxide from a reciprocating spark ignition engine. Fuels considered in the investigation were representative of the aromatic, paraffin, cycloparaffin, and olefin families.</div> <div class="htmlview paragraph">Results show that both energy content and hydrogen/carbon ratio of the fuel are instrumental in predicting the carbon monoxide and nitric oxide concentrations present in the exhaust. In addition, it has been shown that a progressive change from paraffinicity to aromaticity is accompanied by an increase in nitric oxide and, to a lesser extent, an increase in carbon monoxide exhaust concentrations.</div>
Two-section Semiconductor Optical Amplifier Power equaliser with 8dBm Output Saturation Power For 10Gbit/s Applications (1999)

Optical functions such as amplification, switching and wavelength conversion based on Semiconductor Optical Amplifiers (SOA’s) are potentially attractive for optical fibre system. Among them, interferometric wavelength conversion based on cross-phase modulation in SOA’s has demonstrated regenerative and cascadability properties [1-4].
STEREOSPECIFICITY OF 2,4‐DIAMINOBUTYRIC ACID WITH RESPECT TO INHIBITION OF 4‐AMINOBUTYRIC ACID UPTAKE AND BINDING (1977)

S(+)-2,4-Diaminobutyric acid is at least 20 times more potent than the R(-) stereoisomer as an inhibitor of the sodium-dependent uptake of 4-aminobutyric acid (GABA) in rat brain slices. Both isomers, however, are equipotent as inhibitors of sodium-independent binding of GABA to membranes from rat brain. The latter finding may be relevant to the reported neurotoxicity in rats of both isomers of 2,4-diaminobutyric acid after intracisternal injection.
Proposal for a two-qubit quantum phase gate for quantum photonic integrated circuits (2012)

We propose a protocol for a two-qubit quantum phase gate based upon the reflection of photon pulses from a quantum dot in a cavity. Depending on the state of the quantum dot the reflected photons acquire a conditional phase shift. The key ingredient is the ultrafast control of the quantum dot energies by electric fields, which allows for tuning the exciton and biexciton successively into resonance with the cavity mode. The complete dynamics of the gate are simulated, revealing a fidelity of about 0.9. The proposed scheme uses position coding and is therefore well suited to the implementation in an integrated photonic quantum processor.
Research and Development of Facility Agriculture Precision Irrigation Monitor and Control System (2005)

The system is designed aiming at the status of the rapid development of facility agriculture in recent years in China while lacking advanced and practical water-saving technologies and equipments about facility agriculture, by using soil moisture sensor, plant nutrition monitor device, accurate irrigation machine, PLC intelligent controller and computer monitor and control system, The resources of water can be utilized perfectly and reasonably, the irrigation efficiency and water utilization ratio can be realized the best effect. Through application of high precision sensor technology, communication technology, data-base restore and processing technology, the system integrate measure system, irrigation equipment, plant growth, soil moisture with laws of plant growing and water needing, so the control means are more reasonably. It can offer plant irrigation data online and can increase the outputs while saving water, so the precise irrigation can be realized. The system possesses advantages of economic and saving energy, easily operation and high-extent automation, it has character of innovation in Chinese facility agriculture and can be used in greenhouses, tunnels, precise agriculture, grassland or pasture and city afforesting etc. It plays important and practical role in implementation of water-saving agriculture.
Genetic engineering of Synechocystis sp. PCC6803 for poly-β-hydroxybutyrate overproduction (2017)

The biosynthesis of poly-β-hydroxybutyrate (PHB) directly from carbon dioxide is a sustainable alternative for non-renewable, petroleum-based polymer production. Synechocystis sp. PCC6803 can naturally accumulate PHB using CO2 as the sole carbon source, particularly when major nutrients such as nitrogen become limiting. Many previous studies have tried to genetically engineer PHB overproduction; mostly by increasing the expression of enzymes directly involved in its biosynthesis pathway. Here, we have instead concentrated on engineering the central carbon metabolism of Synechocystis such that (i) the PHB synthesis pathway becomes deregulated, and/or (ii) the levels of its substrate, acetyl-CoA, were increased. Seven different mutants were constructed harboring, separately or in combination, three different genetic modifications to Synechocystis' metabolic network. These were the deletions of phosphotransacetylase (Pta) and acetyl-CoA hydrolase (Ach), and the expression of a heterologous phosphoketolase (XfpK) from Bifidobacterium breve. The wild type Synechocystis and the derivative strains were compared in terms of biomass and the PHB production capability during photoautotrophic growth. This was performed in a photobioreactor exposed to a diel light/dark rhythm and using standard BG11 as the growth medium. We found that the strain that combined all three genetic modifications, i.e. xfpk overexpression in a double pta and ach deletion background, showed the highest levels of PHB production from all the strains tested here. Encouragingly, the production levels obtained: 232 mg L− 1, ~ 12% (w/w) of the dry biomass weight, and a productivity of 7.3 mg L− 1 d− 1; are to the best of our knowledge, the highest ever reported for PHB production directly from CO2.
Terminal Radar Approach Control: Measures of Voice Communication System Performance (2005)

When the National Airspace System migrates from its current ground infrastructure and voice communications system to one that encompasses ground and satellite systems, digital data will be the principal communications medium. As technological advances lead to innovations in communications system development, those emerging systems will be evaluated against existing performance parameters. Baseline parameters were derived from waveform analysis performed on 8000 pilot and controller transmissions acquired from the five busiest TRACON facilities in the USA. The major findings were that communications occur quickly and with minimal silence between successive transmissions. Disruptions to efficient information transfer can result from blocked, stepped-on, and clipped transmissions — which occurred in 1.2% of the sampled transmissions. Even so, when transmissions were completely blocked an audible alarm (spoken word ‘blocked’ or heterodyning) typically alerted the controllers to their presence. Future systems developers may want to exploit and expand this capability to include stepped-on and clipped transmissions.
Crystal Structure of Human RANKL Complexed with Its Decoy Receptor Osteoprotegerin (2012)

Receptor activator of NF-κB ligand (RANKL), its signaling receptor RANK, and its decoy receptor osteoprotegerin (OPG) constitute a molecular triad that is critical in regulating bone remodeling, and also plays multiple roles in the immune system. OPG binds RANKL directly to block its interaction with RANK. In this article, we report the 2.7-Å crystal structure of human RANKL trimer in complex with the N-terminal fragment of human OPG containing four cysteine-rich TNFR homologous domains (OPG-CRD). The structure shows that RANKL trimer uses three equivalent grooves between two neighboring monomers to interact with three OPG-CRD monomers symmetrically. A loop from the CRD3 domain of OPG-CRD inserts into the shallow groove of RANKL, providing the major binding determinant that is further confirmed by affinity measurement and osteoclast differentiation assay. These results, together with a previously reported mouse RANKL/RANK complex structure, reveal that OPG exerts its decoy receptor function by directly blocking the accessibilities of important interacting residues of RANKL for RANK recognition. Structural comparison with TRAIL/death receptor 5 complex also reveals structural basis for the cross-reactivity of OPG to TRAIL.