gebhart/patrepeval huggingface dataset:
Mutants of <i>Escherichia coli</i> Defective in Membrane Phospholipid Synthesis: Macromolecular Synthesis in an sn-Glycerol 3-Phosphate Acyltransferase <i> K <sub>m</sub> </i> Mutant (1974)

sn-Glycerol 3-phosphate (G3P) auxotrophs of Escherichia coli have been selected from a strain which cannot aerobically catabolize G3P. The auxotrophy resulted from loss of the biosynthetic G3P dehydrogenase (EC 1.1.1.8) or from a defective membranous G3P acyltransferase. The apparent K(m) of the acyltransferase for G3P was 11- to 14-fold higher (from about 90 mum to 1,000 to 1,250 mum) in membrane preparations from the mutants than those of the parent. All extracts prepared from revertants of the G3P dehydrogenase mutants showed G3P dehydrogenase activity, but most contained less than 10% of the wild-type level. Membrane preparations from revertants of the acyltransferase mutants had apparent K(m)'s for G3P similar to that of the parent. Strains have been derived in which the G3P requirement can be satisfied with glycerol in the presence of glucose, presumably because the glycerol kinase was desensitized to inhibition by fructose 1,6-diphosphate. Investigations on the growth and macromolecular synthesis in a G3P acyltransferase K(m) mutant revealed that upon glycerol deprivation, net phospholipid synthesis stopped immediately; growth continued for about one doubling; net ribonucleic acid (RNA), deoxyribonucleic acid (DNA), and protein nearly doubled paralleling the growth curve; the rate of phospholipid synthesis assessed by labeling cells with (32)P-phosphate, (14)C-acetate, or (3)H-serine was reduced greater than 90%; the rates of RNA and DNA synthesis increased as the cells grew and then decreased as the cells stopped growing; the rate of protein synthesis showed no increase and declined more slowly than the rates of RNA and DNA synthesis when the cells stopped growing. The cells retained and gained in the capacity to synthesize phospholipids upon glycerol deprivation. These data indicate that net phospholipid synthesis is not required for continued macromolecular synthesis for about one doubling, and that the rates of these processes are not coupled during this time period.
Electromagnetic optimization of EMS-MAGLEV systems (1998)

In EMS-MAGLEV high-speed transport systems, devices for propulsion, levitation and contactless on-board electric power transfer are combined in a single electromagnetic structure. The strong coupling among the windings affects the performance of each device and requires the utilization of numerical codes. The paper describes an overall optimization procedure, based on a suitable mathematical model of the system, which takes into account several items of the system performance. The parameters of the model are calculated by an automated sequence of FEM analyses of the configuration. Both the linear generator output characteristics and the propulsion force ripple are improved applying the procedure to a reference configuration. The results are compared with the results obtained by a sequence of partial optimizations operating separately on two different subsets of the geometric parameters.
Introducing problem-based learning as a learning strategy for Masters students (2012)

The introduction of problem-based learning (PBL) as an innovative strategy is often challenging to both teaching staff and students. This is particularly the case when it is only used on one module within a programme. This case study reports on an evaluation of the experiences of students and staff in the first cohort introduced to problem-based learning in one module of an accelerated taught Masters level programme in occupational therapy. Most students responded positively to the module with an acknowledged development in personal and professional skills, team working skills, knowledge base, independent learning skills and successful completion of the module. For a minority of students, however, this was not the case. This paper identifies strategies for effective introduction of a problem-based learning approach to a single module and considers how the potential negative impact on some learners might be minimised.
Techniques for speeding up high-quality perspective maximum intensity projection (1994)

This paper presents three classes of speed-up techniques for calculating high-quality perspective Maximum Intensity Projection (MIP) images from Magnetic Resonance Angiography (MRA) data. The method of choice is a combination of Ray Acceleration by Distance Coding (RADC) with Closest Vessel Projection (CVP); this technique combines a five- to ten-fold speed improvement with improved visualization of blood vessels.
First High Pressure and High Temperature Digital Electric Intellitite Welded Permanent Down Hole Monitoring System for Gas Wells (2008)

Abstract This paper describes an innovative and reliable first High Pressure High Temperature digital electric permanent monitoring solution with state-of-the-art intellitite welded system that allows bottom hole pressure and temperature in real time in JFYN-01 gas well. Permanent down hole system provide bottom hole pressures and temperatures during the producing life of the well both in flowing and shut in conditions which allows to optimize production and flow above dew point in deep high pressure and high temperature wells where intervention is very expensive. The down hole gauge system is connected from wellsite to gas plant through SCADA which allows to monitor well performance in real time. This data are used for history matching, deliverability and transit testing. Included in the paper are equipment selection, design, development details, installation procedures, and "lessons learned" after installation of the fully welded digital permanent down hole monitoring system. Using HPHT permanent system will increase recovery factor and allows producing the well at optimum rate; i.e., above dew point pressure. Ultimately, PDHMS will limit the future interventions.
Dual phase high-temperature membranes for CO<sub>2</sub> separation – performance assessment in post- and pre-combustion processes (2016)

Dual phase membranes are highly CO<sub>2</sub>-selective membranes with an operating temperature above 400 °C. The focus of this work is to quantify the potential of dual phase membranes in pre- and post-combustion CO<sub>2</sub> capture processes. The process evaluations show that the dual phase membranes integrated with an NGCC power plant for CO<sub>2</sub> capture are not competitive with the MEA process for post-combustion capture. However, dual phase membrane concepts outperform the reference Selexol technology for pre-combustion CO<sub>2</sub> capture in an IGCC process. The two processes evaluated in this work, post-combustion NGCC and pre-combustion IGCC, represent extremes in CO<sub>2</sub> partial pressure fed to the separation unit. Based on the evaluations it is expected that dual phase membranes could be competitive for post-combustion capture from a pulverized coal fired power plant (PCC) and pre-combustion capture from an Integrated Reforming Cycle (IRCC).
Wireless sensor network for aircraft health monitoring (2004)

Wireless sensor networks is an emerging paradigm of computing and networking where a node may be selfpowered, and have sensing, computing, and communication capabilities. They have been proposed for use in a wide variety of applications. The objective of this article is to describe a wireless sensor network for monitoring of the health of aircraft engines. We describe the architecture of the wireless sensor network along with how it fits into the general area of wireless sensor networks.
A Mild and Efficient Sonochemical<i>tert</i>-Butoxycarbonylation of Amines from Their Salts (1991)

The introduction of the tert-butoxycarbonyl protecting group onto amines, as free bases or as salts, can be effected under mild conditions with ultrasonic irradiation. This method permits easy work-up and gives the protected compounds in high yield.
A Variant X-linked Chronic Granulomatous Disease Patient (X91+) with Partially Functional Cytochrome b (1995)

Genetic analysis of a patient with the variant cytochrome b-245-positive form of chronic granulomatous disease revealed a missense mutation resulting in a Arg54-->Ser substitution in the gp91phox subunit of cytochrome b-245. As a consequence, although no O2- is made, NADPH oxidase-associated FAD accepts electrons from NADPH in the cell-free activation system and becomes reduced. The reduced flavin exhibits normal levels of iodonitrotetrazolium violet diaphorase activity, and the patient's neutrophils exhibit high levels of intracellular oxidant production and show a low level of NBT staining in the NBT slide test. Thus, this mutation appears to render the heme center of NADPH oxidase present but nonfunctional, while leaving the flavin center fully functional.
A Wireless Electroceutical Dressing Lowers Cost of Negative Pressure Wound Therapy (2015)

<b>Objective:</b> To test whether the use of a wireless electroceutical dressing (WED) (Procellera<sup>®</sup>) in conjunction with a 5-day negative pressure wound therapy (NPWT) may reduce the number of dressing changes required per week with this therapy. <b>Approach:</b> At the Ohio State University Comprehensive Wound Center, chronic wound patients (<i>n</i>=30) undergoing NPWT were randomized into two arms following consent as approved by the institutional review board. The control arm received standard of care NPWT, where the dressing change was performed thrice a week. The test arm received the same care except that the WED was added as an interface layer and dressing change was limited to twice a week. <b>Results:</b> A reduced cost of care was achieved using the WED in conjunction with NPWT. Despite fewer dressing changes in wounds dressed with the WED, closure outcomes were comparable with no overt signs of any wound complication, including infection. The cost of NPWT care during the week was significantly lower (from $2918 to $2346) in the WED-treated group compared with patients in the control arm. <b>Innovation:</b> This work introduces a novel technology platform involving a WED, which may be used in conjunction with NPWT. If used as such, NPWT is effective in decreasing the frequency of dressing change and lowering the cost of care. <b>Conclusion:</b> This work points toward the benefit of using the WED combined with NPWT. A larger clinical trial investigating the cost-effectiveness of WED in wound care is warranted.
Estrone Sulfatase:  Probing Structural Requirements for Substrate and Inhibitor Recognition (1997)

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTEstrone Sulfatase: Probing Structural Requirements for Substrate and Inhibitor RecognitionCynthia Anderson, Jennifer Freeman, Linda H. Lucas, Michael Farley, Habib Dalhoumi, and Theodore S. WidlanskiCite this: Biochemistry 1997, 36, 38, 11542Publication Date (Web):September 23, 1997Publication History Published online23 September 1997Published inissue 1 September 1997https://doi.org/10.1021/bi9750089Copyright © 1997 American Chemical SocietyRequest reuse permissions This publication is free to access through this site. Learn MoreArticle Views231Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (30 KB) Get e-AlertscloseSupporting Info ()»Supporting Information Supporting Information Get e-Alerts
The Revival of the Notes Field: Leveraging the Unstructured Content in Electronic Health Records (2019)

<b>Problem:</b> Clinical practice requires the production of a time- and resource-consuming great amount of notes. They contain relevant information, but their secondary use is almost impossible, due to their unstructured nature. Researchers are trying to address this problems, with traditional and promising novel techniques. Application in real hospital settings seems not to be possible yet, though, both because of relatively small and dirty dataset, and for the lack of language-specific pre-trained models. <b>Aim:</b> Our aim is to demonstrate the potential of the above techniques, but also raise awareness of the still open challenges that the scientific communities of IT and medical practitioners must jointly address to realize the full potential of unstructured content that is daily produced and digitized in hospital settings, both to improve its data quality and leverage the insights from data-driven predictive models. <b>Methods:</b> To this extent, we present a narrative literature review of the most recent and relevant contributions to leverage the application of Natural Language Processing techniques to the free-text content electronic patient records. In particular, we focused on four selected application domains, namely: data quality, information extraction, sentiment analysis and predictive models, and automated patient cohort selection. Then, we will present a few empirical studies that we undertook at a major teaching hospital specializing in musculoskeletal diseases. <b>Results:</b> We provide the reader with some simple and affordable pipelines, which demonstrate the feasibility of reaching literature performance levels with a single institution non-English dataset. In such a way, we bridged literature and real world needs, performing a step further toward the revival of notes fields.
The Glutamine-rich Region of the HIV-1 Tat Protein Is Involved in T-cell Apoptosis (2004)

Human immunodeficiency virus (HIV) infection and the progression to AIDS are characterized by the depletion of CD4(+) T-cells. HIV-1 infection leads to apoptosis of uninfected bystander cells and the direct killing of HIV-infected cells. This is mediated, in part, by the HIV-1 Tat protein, which is secreted by virally infected cells and taken up by uninfected cells. We chemically synthesized two 86-residue subtype D Tat proteins, Ug05RP and Ug11LTS, from two Ugandan patients who were clinically categorized as either rapid progressor or long-term survivor, with non-conservative mutations located essentially in the glutamine-rich region. Structural heterogeneities were revealed by CD, which translate into differing trans-activational and apoptotic effects. CD data analysis and molecular modeling indicated that the short alpha-helix observed in subtype D Tat proteins from rapid progressor patients such as Tat Mal and Tat Ug05RP is not present in Ug11LTS. We show that Tat Ug05RP is more efficient than Tat Ug11LTS in its trans-activational role and in inducing apoptosis in binding tubulin via the mitochondrial pathway. The glutamine-rich region of Tat appears to be involved in the Tat-mediated apoptosis of T-cells.
High-Throughput Sample Preparation from Whole Blood for Gene Expression Analysis (2006)

Whole blood is an attractive sample source for nucleic acid-based assays because it is readily available, easily accessible, and rich in genetic information. However, globin mRNA accounts for up to 70% of the mRNA (by mass) in whole blood total RNA, resulting in distortion of the RNA amplification and subsequently causing decreased Present calls, decreased call concordance, and increased signal variation in microarray analysis. Therefore, for gene expression analysis, whole blood is typically fractionated before total RNA isolation to reduce globin mRNA content. We have developed a high-throughput sample preparation technology that streamlines workflows for (1) total RNA isolation from whole blood (MagMAX-96 Blood RNA Isolation Kit), (2) globin mRNA removal using a novel, nonenzymatic technology (GLOBINclear—Human Kit), and (3) mRNA amplification and labeling for expression analysis (MessageAmp II-96 aRNA Amplification Kit). Globin mRNA removal eliminates the need for prefractionation of whole blood, minimizing the potential for expression profile changes during sample handling. Quantitative RT-PCR showed that this method effectively removed up to 95% of the globin mRNA from the isolated RNA while retaining normal levels of other mRNAs. The streamlined sample preparation enables quick and accurate expression analysis of relatively high numbers of blood samples.
Coating of TiO<sub>2</sub> particles by chemical vapor deposition of SiO<sub>2</sub> (1996)

A simple CVD process for coating titanium dioxide particles to improve dispersion , pigment durability, and resistance to viscosity increases in paints is presented. The conventionally used liquid‐phase routes are complicated and expensive and often lead to porous layers, which have high oil absorption, whereas the continuous, high temperature gas phase route described here produces dense layers of silica. The Figure shows a possible mechanism for the coating process. magnified image
Drosophila: A Laboratory Handbook. By Michael Ashburner. New York: Cold Spring Harbor Laboratory. 1989. 1331 pages. Price $180.00. ISBN 0 87969 321 5. - Drosophila: A Laboratory Manual. By Michael Ashburner. New York: Cold Spring Harbor Laboratory. 1989. 434 pages. Price $50.00. ISBN 0 97969 322 3. Price for the set: $230.00. (1990)

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Variable self-powered light detection CMOS chip with real-time adaptive tracking digital output based on a novel on-chip sensor (2017)

This paper provides a solution for a self-powered light direction detection with digitized output. Light direction sensors, energy harvesting photodiodes, real-time adaptive tracking digital output unit and other necessary circuits are integrated on a single chip based on a standard 0.18 µm CMOS process. Light direction sensors proposed have an accuracy of 1.8 degree over a 120 degree range. In order to improve the accuracy, a compensation circuit is presented for photodiodes' forward currents. The actual measurement precision of output is approximately 7 ENOB. Besides that, an adaptive under voltage protection circuit is designed for variable supply power which may undulate with temperature and process.
Identification of the first archaeal Type 1 RNase H gene from <i>Halobacterium</i> sp. NRC-1: archaeal RNase HI can cleave an RNA–DNA junction (2004)

All the archaeal genomes sequenced to date contain a single Type 2 RNase H gene. We found that the genome of a halophilic archaeon, Halobacterium sp. NRC-1, contains an open reading frame with similarity to Type 1 RNase H. The protein encoded by the Vng0255c gene, possessed amino acid sequence identities of 33% with Escherichia coli RNase HI and 34% with a Bacillus subtilis RNase HI homologue. The B. subtilis RNase HI homologue, however, lacks amino acid sequences corresponding to a basic protrusion region of the E. coli RNase HI, and the Vng0255c has the similar deletion. As this deletion apparently conferred a complete loss of RNase H activity on the B. subtilis RNase HI homologue protein, the Vng0255c product was expected to exhibit no RNase H activity. However, the purified recombinant Vng0255c protein specifically cleaved an RNA strand of the RNA/DNA hybrid in vitro, and when the Vng0255c gene was expressed in an E. coli strain MIC2067 it could suppress the temperature-sensitive growth defect associated with the loss of RNase H enzymes of this strain. These results in vitro and in vivo strongly indicate that the Halobacterium Vng0255c is the first archaeal Type 1 RNase H. This enzyme, unlike other Type 1 RNases H, was able to cleave an Okazaki fragment-like substrate at the junction between the 3'-side of ribonucleotide and 5'-side of deoxyribonucleotide. It is likely that the archaeal Type 1 RNase H plays a role in the removal of the last ribonucleotide of the RNA primer from the Okazaki fragment during DNA replication.
Specificity of Antibodies and Tuberculin Response after Occupational Exposure to Tuberculosis (1992)

Specific antibody levels and delayed-type hypersensitivity skin responses to antigens of Mycobacterium tuberculosis in 39 hospital staff who were heavily exposed to tuberculosis (TB) were compared with those in 36 factory employees from Indonesia. Antibody levels to the TB68 epitope of the 14-kDa antigen were significantly greater, while titers to the TB23 (19-kDa) and TB72 (38-kDa) epitopes and lipoarabinomannan (LAM) were lower in exposed than in nonexposed subjects (all P less than .02). The intensity of tuberculin responses correlated positively with anti-LAM and negatively with anti-19-kDa antibody levels. Possible reasons for the selective humoral response of chronically exposed healthy subjects to the 14-kDa antigen, but not to other antigens immunogenic in patients with tuberculosis, are discussed.
To the Editor: (1971)

Journal Article To the Editor: Get access V R Mallikarjuneswara, V R Mallikarjuneswara Department of Obstetrics and Gynecology Methodist Hospital of Brooklyn Brooklyn, N.Y. 11215 Search for other works by this author on: Oxford Academic Google Scholar C A B Clemetson, C A B Clemetson Department of Obstetrics and Gynecology Methodist Hospital of Brooklyn Brooklyn, N.Y. 11215 Search for other works by this author on: Oxford Academic Google Scholar J J Carr J J Carr Department of Laboratories Methodist Hospital of Brooklyn Brooklyn, N.Y. 11215 Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 17, Issue 5, 1 May 1971, Page 458, https://doi.org/10.1093/clinchem/17.5.458a Published: 01 May 1971
Analysis of unsteady airfoil interference effects using a zonal Navier-Stokes solver (1995)

© 1995, by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved.Unsteady aerodynamic interference effects and thrust generation on a flapping/stationary airfoil combination in tandem are studied parametrically. A zonal Navier-Stokes solver is employed to compute the unsteady flowfield. The computational domain is discretized with overlapping uniform grids generated around each airfoil. The thrust generation and the propulsive efficiency of the flapping/stationary airfoil combination is found to be a strong function of reduced frequency and the amplitude of the flapping motion of the leading airfoil. The maximum propulsive efficiency of a NACA0012 airfoil combination has been computed to be 70% at a reduced frequency of 0.75 and a flapping amplitude of 0.20 chord lengths when the airfoils are separated by about two chord lengths.
Arabic calligraphy recognition based on binarization methods and degraded images (2011)

Optical Font Recognition is one of the main challenges in this time. The available methods of optical font recognition are deal with the recent documents and fonts types. However, there are neglected in dealing with the historical and regarded documents. Moreover, they have neglected languages that are not belong into Asian or Latin. Regarding to those types of documents, we proposed a new framework of optical font recognition for Arabic calligraphy. We enhance binarization method based on previous works. By introducing that, we achieve better quality images at the preprocessing stage. Then we generate text block before passing mailing to post-processing stages. Then, we extract the features based on edge direction matrixes. In the classification stage, we apply backpropagation neural network to identify the font type of the calligraphy. We observe that our proposal method achieve better performance in both preprocessing and post processing.