gebhart/patrepeval huggingface dataset:
Surface-Enhanced Raman Spectroscopy for Bioanalysis: Reliability and Challenges (2018)

Surface-enhanced Raman spectroscopy (SERS) inherits the rich chemical fingerprint information on Raman spectroscopy and gains sensitivity by plasmon-enhanced excitation and scattering. In particular, most Raman peaks have a narrow width suitable for multiplex analysis, and the measurements can be conveniently made under ambient and aqueous conditions. These merits make SERS a very promising technique for studying complex biological systems, and SERS has attracted increasing interest in biorelated analysis. However, there are still great challenges that need to be addressed until it can be widely accepted by the biorelated communities, answer interesting biological questions, and solve fatal clinical problems. SERS applications in bioanalysis involve the complex interactions of plasmonic nanomaterials with biological systems and their environments. The reliability becomes the key issue of bioanalytical SERS in order to extract meaningful information from SERS data. This review provides a comprehensive overview of bioanalytical SERS with the main focus on the reliability issue. We first introduce the mechanism of SERS to guide the design of reliable SERS experiments with high detection sensitivity. We then introduce the current understanding of the interaction of nanomaterials with biological systems, mainly living cells, to guide the design of functionalized SERS nanoparticles for target detection. We further introduce the current status of label-free (direct) and labeled (indirect) SERS detections, for systems from biomolecules, to pathogens, to living cells, and we discuss the potential interferences from experimental design, measurement conditions, and data analysis. In the end, we give an outlook of the key challenges in bioanalytical SERS, including reproducibility, sensitivity, and spatial and time resolution.
<i>dishevelled</i> is a component of the <i>frizzled</i> signaling pathway in <i>Drosophila</i> (1995)

The tissue polarity genes in Drosophila are required to coordinate cell polarity within the plane of the epidermis. Evidence to date suggests that these genes may encode components of a novel signal transduction pathway. Three of the genes, frizzled (fz), dishevelled (dsh), and prickle (pk) share a similar tissue polarity phenotype, suggesting that they function together in a single process. dsh is also known to function as a mediator of wingless (wg) signaling in a variety of developmental patterning processes in the fly. In this study, we make use of a fz transgene and a hypomorphic fz allele as genetic tools in an attempt to order these genes in a genetic hierarchy. Our results argue that dsh encodes a dosage sensitive component required for fz function and that it likely acts downstream of fz in the generation of tissue polarity. Our findings suggest that dsh may have a general role in signal transduction, perhaps as a component of a receptor complex.
Nonsurgical correction of congenital ear abnormalities in the newborn: Case series (2005)

A simple, nonsurgical treatment in a Caucasian population appeared to be very effective in correcting congenital ear abnormalities with no complications and high patient/parent satisfaction.
Effect of aberrations and scatter on image resolution assessed by adaptive optics retinal section imaging (2007)

The effect of increased high-order wavefront aberrations on image resolution was investigated, and the performance of adaptive optics (AO) for correcting wavefront error in the presence of increased light scatter was assessed in a model eye. An AO section imaging system provided an oblique view of a model retina and incorporated a wavefront sensor and deformable mirror for measurement and compensation of wavefront aberrations. Image resolution was quantified by the width of a Lorentzian curve fitted to a laser line image. Wavefront aberrations were significantly reduced with AO, resulting in improvement of image resolution. In the model eye, image resolution was degraded with increased high-order wavefront aberrations (horizontal coma and spherical) and improved with AO correction of wavefront error in the presence of increased light scatter. The findings of the current study suggest that AO imaging systems can potentially improve image resolution in aging eyes with increased aberrations and scatter.
Novel mutations in the connexin 32 gene associated with X‐linked Charcot‐Marie tooth disease (1996)

Charcot-Marie-Tooth disease is a pathologically and genetically hetergenous group of disorders that cause a progressive neuropathy, defined pathologically by degeneration of the myelin (CMT 1) of the axon (CMT 2) of the peripheral nerves. An X-linked type of the demyelinating form of this disorder (CMT X) has recently been linked to mutations in the connexin 32 (Cx32) gene, which codes for a 284 amino acid gap junction protein found in myelinated peripheral nerve. To date some 7 different mutations in this gene have been identified as being responsible for CMT X. The majority of these predict nonconservative amino acid substitutions, while one is a frameshift mutation which predicts a premature stop at codon 21. We report the results of molecular studies on three further local CMT X kindreds. The Cx32 gene was amplified by PCR in three overlapping fragments 300-450 bp in length using leukocyte-derived DNA as template. These were either sequenced directly using a deaza dGTP sequencing protocol, or were cloned and sequenced using a TA vector. In two of the kindreds the affected members carried a point mutation which was predicted to effect a non-conservative amino acid change within the first transmembrane domain. Both of these mutations causedmore » a restriction site alteration (the loss of an Nla III and the creation of a Pvu II, respectively), and the former mutation was observed to segregate with the clinicial phenotype in affected family members. Affected members of the third kindred, which was a very large multigenerational family that had been extensively studied previously, were shown to carry a point mutation predicted to cause a premature truncation of the Cx32 gene product in the intracellular carboxy terminus. This mutation obliterated an Rsa I site which allowed a rapid screen of several other family members.« less
Terferol, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase. II Structural elucidation. (1984)

Streptomyces showdoensis SANK 65080 produced terferol, an inhibitor of cyclic adenosine 3',5'-monophosphate phosphodiesterase (cAMP-PDE). NMR spectrometry and X-ray analysis were used to determine the structure of the compound, a new member of the terphenyl family.
HPDA: A hybrid parity-based disk array for enhanced performance and reliability (2010)

A single flash-based Solid State Drive (SSD) can not satisfy the capacity, performance and reliability requirements of a modern storage system supporting increasingly demanding data-intensive computing applications. Applying RAID schemes to SSDs to meet these requirements, while a logical and viable solution, faces many challenges. In this paper, we propose a Hybrid Parity-based Disk Array architecture, HPDA, which combines a group of SSDs and two hard disk drives (HDDs) to improve the performance and reliability of SSD-based storage systems. In HPDA, the SSDs (data disks) and part of one HDD (parity disk) compose a RAID4 disk array. Meanwhile, a second HDD and the free space of the parity disk are mirrored to form a RAID1-style write buffer that temporarily absorbs the small write requests and acts as a surrogate set during recovery when a disk fails. The write data is reclaimed back to the data disks during the lightly loaded or idle periods of the system. Reliability analysis shows that the reliability of HPDA, in terms of MTTDL (Mean Time To Data Loss), is better than that of either pure HDD-based or SSD-based disk array. Our prototype implementation of HPDA and performance evaluations show that HPDA significantly outperforms either HDD-based or SSD-based disk array.
Quick and highly efficient copper-catalyzed cycloaddition of aliphatic and aryl azides with terminal alkynes “on water” (2008)

We have developed a quick and highly efficient method for copper-catalyzed cycloaddition of water-insoluble aliphatic/aryl azides and alkynes “on water” at room temperature, the protocol uses 10 mol% CuBr as the catalyst, and 50 mol% PhSMe as the ligand.
Enabling Hand-Crafted Visual Markers at Scale (2017)

As locative media and augmented reality spread into the everyday world so it becomes important to create aesthetic visual markers at scale. We explore a designer-centred approach in which skilled designers handcraft seed designs that are automatically recombined to create many markers as subtle variants of a common theme. First, we extend the d-touch topological approach to creating visual markers that has previously been shown to support creative design with two new techniques: area order codes and visual checksums. We then show how the topological structure of such markers provides the basis for recombining designs to generate many variations. We demonstrate our approach through the creation of beautiful, personalized and interactive wallpaper. We reflect on how technologies must enable designers to balance goals of scalability, aesthetics and reliability in creating beautiful interactive decoration.
Effect of Lactobacillus Plantarum TWK10 on Improving Endurance Performance in Humans (2018)

Microbiota is currently an important issue in disease and health and many studies have revealed it to play an important role in physiological homeostasis and health promotion. Lactobacillus plantarum (L. plantarum), isolated from Taiwan pickled vegetables, is a well-known probiotic microorganism. In a recent animal study, it was shown that supplementation of mice with L. plantarum TWK10 (TWK10) could increase muscle mass, improve exercise performance and exert anti-fatigue effects. In order to examine the ergogenic effect of TWK10 supplementation on endurance performance in humans, we conducted a human double-blind placebo-controlled clinical study. A total of sixteen adult subjects over 20 years of age were recruited and randomly allocated to the placebo or TWK10 group (n = 8 each). The TWK10 group received 6 weeks of supplementation. Physiological assessments were conducted by exhaustive treadmill exercise measurements and related biochemical indexes. After 6 weeks of supplementation, levels of lactic acid, blood ammonia, blood glucose, free fatty acid (FFA) and creatine kinase (CK) were evaluated during exhaustive exercise. We were able to show that the TWK10 group had significantly higher endurance performance and glucose content in a maximal treadmill running test compared to the placebo group (P < 0.05), suggesting that TWK10 supplementation may be beneficial to energy harvest. Taken together, our results suggest that TWK10 has the potential to be an aerobic exercise supplement for physiological adaptation or an ergogenic supplement with health benefits for amateur runners.
Extraordinary Room-Temperature Photoluminescence in Triangular WS<sub>2</sub> Monolayers (2012)

Individual monolayers of metal dichalcogenides are atomically thin two-dimensional crystals with attractive physical properties different from those of their bulk counterparts. Here we describe the direct synthesis of WS2 monolayers with triangular morphologies and strong room-temperature photoluminescence (PL). The Raman response as well as the luminescence as a function of the number of S-W-S layers is also reported. The PL weakens with increasing number of layers due to a transition from direct band gap in a monolayer to indirect gap in multilayers. The edges of WS2 monolayers exhibit PL signals with extraordinary intensity, around 25 times stronger than that at the platelet's center. The structure and chemical composition of the platelet edges appear to be critical for PL enhancement.
Error flatten logarithm approximation for graphics processing unit (2011)

This paper propose a high performance algorithm for logarithm approximation. A piecewise linear interpolation with non-uniform partition is introduced to produce a flatten error distribution. Base on error flatten algorithm our method can produce greatly more accurate results than state-of-art logarithm approximation. Our proposed logarithm conversion is further optimized for hardware implementation. Analysis of simulation results in Matlab and hardware resources in FPGA illustrate that our method are superior or comparable to state-of-art logarithm conversion for graphics hardware.
Phytoestrogenic activity of blackcurrant (<i>Ribes nigrum</i>) anthocyanins is mediated through estrogen receptor alpha (2015)

These results suggest that blackcurrant anthocyanins act as phytoestrogens in vitro and in vivo.
ChemInform Abstract: Review on Synthesis of 3‐Substituted 1,2‐Benzisoxazole Derivatives (2016)

Abstract Review: 29 refs.
Dextran metabolism following infusion of 7.5% NaCl/6% dextran‐70 to euvolemic and hemorrhaged rabbits (1992)

Abstract Dextran metabolism was evaluated in euvolemic and hemorrhaged rabbits following administration of a 7.5%NaCl/6% Dextran‐70 (HSD) solution. Control rabbits and those bled 8 ml/kg body weight were infused i.v. with 4 ml/kg of HSD or HSD containing 14 C‐Dextran‐70. Blood samples were withdrawn prior to and at times up to 96 hr after HSD infusion. Peak serum dextran concentrations were about 29% higher in hemorrhaged rabbits than in controls, yet serum dextran t 1/2 was similar in both groups. Molecular weight (MW) distribution of dextran in serum showed a slight shift toward a MW >70,000, consistent with excretion of lower MW forms in the urine. After 96 hr concentrations of 14 C‐Dextran were 20‐fold higher in liver from both groups of rabbits, in comparison to spleen, lung, and kidney. In addition, dextranase activity in liver was markedly higher than in the other tissues assayed. These studies indicate that dextran infused as HSD does not associate with any protein fractions, is found only in low concentrations in tissue, and has a serum half‐life adequate to serve as a useful plasma volume expander.
Low molecular weight GTP-binding proteins are associated with neuronal organelles involved in rapid axonal transport and exocytosis (1989)

Recent evidence suggests that low molecular weight GTP-binding proteins may play important roles in a variety of membrane transport processes. In order to address the question of whether these proteins are involved in transport processes in the nerve axon, we have assessed their presence in rapid transport membranes from rabbit optic nerve. We report the characterization of a group of low molecular weight GTP-binding proteins which are constituents of rapid transport vesicles. Although these proteins are components of rapid transport vesicles, they are apparently not major rapidly transported species. They are localized in cytosolic as well as in membrane fractions of axons, and the membrane-associated form behaves as an integral membrane protein(s). These proteins are also found in association with a variety of vesicular and organellar components of neurons including coated vesicles, synaptic vesicles, synaptic plasma membranes, and mitochondria. We discuss the possible roles of these proteins in rapid axonal transport and exocytosis.
The Differential Glycosylation of Human Pro-Urokinase from Various Recombinant Mammalian Cell Lines Does Not Affect Activity and Binding to PAI-1 (1989)

Human chromosomal DNA encoding single-chain urokinase-type Plasminogen Activator (scu-PA, or pro-urokinase) was inserted in an expression plasmid and transfected in human A431, mouse LB6 and CHO cells. LB6 cells were also transfected with a Bovine Papilloma Virus derivative containing the scu-PA gene. Human scu-PA was purified from cell supernatants of recombinant clones and characterized for structure and function. All recombinant scu-PAs are undistinguishable from human urine-derived scu-PA for peptide backbone, but possess a higher sugar content, as revealed by SDS-PAGE analysis after digestion with glycopeptidase F. This difference is partly due to an increased sialic acid content, as shown by analysis of neuraminidase-treated scu-PAs. No difference was found, however, among recombinant and natural scu-PAs in the kinetics of conversion into two-chain active forms (tcu-PAs) by human plasmin, and in the KM and kcat values of tcu-PA activity on the chromogenic substrate S-2444 and on human plasminogen. Also, recombinant and non-recombinant tcu-PAs displayed similar dose-response curves for binding to the endothelial inhibitor PAI-1. In conclusion, the glycosylation pattern of u-PA does not affect its interaction with the plasma proteins directly involved in its fibrinolytic function.
Ecologic analysis of some immune-related disorders, including type 1 diabetes, in Australia: latitude, regional ultraviolet radiation, and disease prevalence. (2002)

The apparent immune-suppressive effect of ultraviolet radiation (UVR) has suggested that this environmental exposure may influence the development of immune-related disorders. Self-reported prevalence rates of type 1 diabetes mellitus, rheumatoid arthritis (RA), eczema/dermatitis, and asthma, from the 1995 Australian National Health Survey, were therefore examined by latitude and ambient level of UVR. A positive association of type 1 diabetes mellitus prevalence was found with both increasing southern latitude of residence (r = 0.77; p = 0.026) and decreasing regional annual ambient UVR (r= -0.80; p = 0.018); a 3-fold increase in prevalence from the northernmost region to the southernmost region was evident. In contrast, asthma correlated negatively with latitude (r = -0.72; p = 0.046), although the change in asthma prevalence from the north to the south of Australia was only 0.7-fold. For both RA and eczema/dermatitis, there were no statistically significant associations between latitude/UVR and disease prevalence. These ecologic data provide some support for a previously proposed beneficial effect of UVR on T-helper 1-mediated autoimmune disorders such as type 1 diabetes. The inverse association of type 1 diabetes prevalence with UVR is consistent with that previously reported for another autoimmune disease, multiple sclerosis, in Australia, and also with type 1 diabetes latitudinal gradients in the Northern Hemisphere. The finding also accords with photoimmunologic evidence of UVR-induced immunosuppression and may suggest a beneficial effect of UVR in reducing the incidence of such autoimmune conditions. In light of this study, analytic epidemiologic studies investigating risk of immune disorders in relation to personal UVR exposure in humans are required.
Determination of the thermal-conductivity tensor and the heat capacity of insulating solids with the transient hot-strip method (1981)

The recently described transient hot-strip method has been developed for the study of solids with direction dependent thermal conductivity. By making three independent measurements, with the hot-strip properly oriented, information can be obtained about the thermal conductivities along the principal directions and also about the specific heat capacity per unit volume. To demonstrate the versatility of the method, crystalline quartz was studied over the temperature range 230–340 K and the results are in good agreement with earlier reported data.
Antagonist Effects of Ca<sup>2+</sup>and Spermine on Phosphatidylinositol 4,5-Bisphosphate-Mediated Transmembrane Redistribution of Phospholipids in Large Unilamellar Vesicles and in Erythrocytes (1996)

We have previously suggested the involvement of a Ca(2+)-phosphatidylinositol 4,5-bisphosphate (PIP2) complex in the phospholipid transmembrane redistribution triggered by cytosolic Ca2+ in erythrocytes. Indeed, the lipid scrambling was induced by extracellular Ca2+ in erythrocytes loaded with PIP2 and was abolished in inside-out vesicles prepared from PIP2-depleted erythrocytes (Sulpice, J.C., Zachowski, A., Devaux, P.F., & Giraud, F. (1994) J. Biol. Chem. 269, 6347-6354). Here, we show that Ca2+ triggers a partial redistribution of spin-labeled phospholipids in protein-free large unilamellar vesicles (LUVs), only when they contain PIP2. Spermine, a polyamine known to interact with PIP2 and reported to inhibit lipid scrambling in resealed ghosts, was found to inhibit also the Ca(2+)-induced scrambling in LUVs and in PIP2-loaded erythrocytes, presumably by interacting with PIP2 and preventing the formation of Ca(2+)-PIP2 complexes. A similar mechanism can account for spermine inhibition in natural membranes, confirming the role of PIP2 in the scrambling process without excluding the participation of proteins. In erythrocytes, activation of the phosphoinositide phospholipase C (PLC) or a 20 h ATP depletion, which both led to a reduction in the PIP2 content by 40-60%, did not affect Ca(2+)-induced phospholipid scrambling. In contrast, longer ATP depletion, resulting in a 80% reduction in the PIP2 content, did induce a significant decrease in lipid scrambling, suggesting that only the PIP2 pool resistant to the PLC was involved. Spermine was able to inhibit hydrolysis of this pool by an exogenous PLA2. It is thus likely that spermine antagonized the Ca(2+)-induced scrambling in resealed ghosts by interacting with the PLC-resistant pool of PIP2.
Rational Design, Synthesis, and Evaluation of Key Analogues of CC-1065 and the Duocarmycins (2007)

The design, synthesis, and evaluation of a predictably more potent analogue of CC-1065 entailing the substitution replacement of a single skeleton atom in the alkylation subunit are disclosed and were conducted on the basis of design principles that emerged from a fundamental parabolic relationship between chemical reactivity and cytotoxic potency. Consistent with projections, the 7-methyl-1,2,8,8a-tetrahydrocyclopropa[c]thieno[3,2-e]indol-4-one (MeCTI) alkylation subunit and its isomer 6-methyl-1,2,8,8a-tetrahydrocyclopropa[c]thieno[2,3-e]indol-4-one (iso-MeCTI) were found to be 5-6 times more stable than the MeCPI alkylation subunit found in CC-1065 and slightly more stable than even the DSA alkylation subunit found in duocarmycin SA, placing it at the point of optimally balanced stability and reactivity for this class of antitumor agents. Their incorporation into the key analogues of the natural products provided derivatives that surpassed the potency of MeCPI derivatives (3-10-fold), matching or slightly exceeding the potency of the corresponding DSA derivatives, consistent with projections made on the basis of the parabolic relationship. Notable of these, MeCTI-TMI proved to be as potent as or slightly more potent than the natural product duocarmycin SA (DSA-TMI, IC50 = 5 vs 8 pM), and MeCTI-PDE2 proved to be 3-fold more potent than the natural product CC-1065 (MeCPI-PDE2, IC50 = 7 vs 20 pM). Both exhibited efficiencies of DNA alkylation that correlate with this enhanced potency without impacting the intrinsic selectivity characteristic of this class of antitumor agents.
Electrostatic Capacitive Imaging: A New NDE Technique (2007)

A new technique for NDE has been developed which is capable of imaging a wide range of materials and structures, ranging from insulators to metallic conductors. The approach, known as Capacitive Imaging (CI) uses electrode arrays in air to produce an AC electric field distribution within the material. Scanning the electrodes over the material causes a change in the field distribution, and hence changes in output voltage. Capacitive coupling allows the technique to work on a wide variety of material conductivities without some of the disadvantages associated with conventional eddy current and potential drop methods. Images are presented of carbon fibre composite materials, concrete and Plexiglas, illustrating the range of application in NDE. The effect of electrode shape and excitation frequency will be discussed in terms of image resolution and depth of penetration.