gebhart/patrepeval huggingface dataset:
The Transcriptome of a Human Polar Body Accurately Reflects Its Sibling Oocyte (2011)

Improved methods are needed to reliably and accurately evaluate oocyte quality prior to fertilization and transfer into the woman of human embryos created through in vitro fertilization (IVF). All oocytes that are retrieved and matured in culture are exposed to sperm with little in the way of evaluating the oocyte quality. Furthermore, embryos created through IVF are currently evaluated for developmental potential by morphology, a criterion lacking in quantitation and accuracy. With the recent successes in oocyte vitrification and storage, clear metrics are needed to determine oocyte quality prior to fertilizing. The first polar body (PB) is extruded from the oocyte before fertilization and can be biopsied without damaging the oocyte. Here, we tested the hypothesis that the PB transcriptome is representative of that of the oocyte. Polar body biopsy was performed on metaphase II (MII) oocytes followed by single-cell transcriptome analysis of the oocyte and its sibling PB. Over 12,700 unique mRNAs and miRNAs from the oocyte samples were compared with the 5,431 mRNAs recovered from the sibling PBs (5,256 shared mRNAs or 97%, including miRNAs). The results show that human PBs reflect the oocyte transcript profile and suggests that mRNA detection and quantification through high-throughput quantitative PCR could result in the first molecular diagnostic for gene expression in MII oocytes. This could allow for both oocyte ranking and embryo preferences in IVF applications. Improved methods are needed to reliably and accurately evaluate oocyte quality prior to fertilization and transfer into the woman of human embryos created through in vitro fertilization (IVF). All oocytes that are retrieved and matured in culture are exposed to sperm with little in the way of evaluating the oocyte quality. Furthermore, embryos created through IVF are currently evaluated for developmental potential by morphology, a criterion lacking in quantitation and accuracy. With the recent successes in oocyte vitrification and storage, clear metrics are needed to determine oocyte quality prior to fertilizing. The first polar body (PB) is extruded from the oocyte before fertilization and can be biopsied without damaging the oocyte. Here, we tested the hypothesis that the PB transcriptome is representative of that of the oocyte. Polar body biopsy was performed on metaphase II (MII) oocytes followed by single-cell transcriptome analysis of the oocyte and its sibling PB. Over 12,700 unique mRNAs and miRNAs from the oocyte samples were compared with the 5,431 mRNAs recovered from the sibling PBs (5,256 shared mRNAs or 97%, including miRNAs). The results show that human PBs reflect the oocyte transcript profile and suggests that mRNA detection and quantification through high-throughput quantitative PCR could result in the first molecular diagnostic for gene expression in MII oocytes. This could allow for both oocyte ranking and embryo preferences in IVF applications.
cDNA cloning and molecular characterization of cherry green ring mottle virus. (1998)

The complete nucleotide sequence of the cherry green ring mottle virus (CGRMV) genome was determined to be 8372 nt excluding a 3' poly(A) tail. Based on computer analysis and sequence comparison, five open reading frames (ORFs) were identified on the virion strand encoding: a putative RNA-dependent RNA polymerase, a triple gene block and a coat protein. Two other ORFs with Mr values over 10,000 and internal to the helicase and coat protein genes, but of unknown function, were also identified. Sequence and genome structure comparisons with other filamentous viruses indicated that CGRMV is most similar to apple stem pitting virus, some carlaviruses and potexviruses. However, it is different from members of any of these virus groups in regard to sequence homology and genome organization. A chimeric fusion coat protein was expressed in E. coli and antibodies specific for the CGRMV coat protein were raised in rabbits. The antibody was used in Western blot analyses to detect the CGRMV coat protein in infected cherry tissue.
"Poisee" and "Goshee" (1928)

"Poisee" and "Goshee" Get access H. B. Morse H. B. Morse 1Camberley Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume CLV, Issue nov03, 3 November 1928, Page 314, https://doi.org/10.1093/nq/CLV.nov03.314d Published: 03 November 1928
Die extrakorporale radikale DaVinci-Prostatektomie (2006)

Die roboterassistierte laparoskopische Prostatektomie ermöglicht die kurative radikale Prostatektomie mit maximaler Gewebeschonung, geringem Blutverlust und sehr guter Erhaltung der erektilen Funktion und Kontinenz. Die Komplikationsrate ist gering. Die Hauptvorteile eines extraperitonealen Zuganges bestehen hauptsächlich in der nur geringen Kopftieflage während der Operation und dem Schutz vor intestinalen und intraabdomalen Komplikationen. Die Erholung des Patienten nach dem Eingriff ist kurz und erlaubt eine baldige Rückkehr zum Arbeitsplatz. Die Zahl der Eingriffe entwickelt sich in den USA exponentiell, während die Technologie im deutschsprachigen Raum erst in wenigen spezialisierten Zentren routinemäßig etabliert ist.
Reconstruction of UTC (NIM) (2013)

NIM has reconstructed UTC (NIM) since 2010 at new campus, and the official UTC (NIM) was switched to the new campus in October 2012. The new clock ensemble includes 6 Hydrogen masers and 7 Cesium clocks, with 1 Hydrogen maser and 2 Cesium clocks placed at the old campus. The master clock is a Hydrogen maser at new campus, and UTC (NIM) is generated from a micro phase stepper steered by a new algorithm and UTC, the frequency of the Hydrogen maser is predicted and rapid UTC data is used to steer UTC (NIM). A dual mixer time difference measurement system is used to measure the time difference between the clocks and UTC (NIM) at new campus, the clocks at old campus are linked to UTC (NIM) by GPS. GPS and TWSTFT data are reported to BIPM everyday automatically. The GPS receivers at new campus are calibrated with reference to one GPS receiver calibrated by BIPM. The UTC (NIM) has been operated normally at new campus; the accuracy and stability are improved. In this paper the generation of new UTC (NIM) is described and the performance of UTC (NIM) is presented.
Mechanism underlying increased neuronal activity in the rat ventrolateral periaqueductal grey by a μ‐opioid (1999)

1. The overall effect of the mu-opioid receptor agonist DAMGO (Tyr-D-Ala-Gly-MePhe-Gly-ol) on ventrolateral periaqueductal grey (PAG) neurons in brain slices was studied using the whole-cell patch-clamp recording technique. 2. Under current-clamp conditions, DAMGO (1 microM) increased cell firing in many PAG neurons even though the opioid induced hyperpolarization and inhibited excitatory postsynaptic potentials (EPSPs) in these cells. 3. The increase in cell activity by DAMGO was observed in both transverse and horizontal slices. The increase persisted when the membrane potential was re-depolarized to the control level. Thus, different planes of sections or the removal of Na+ channel inactivation could not account for the observation. 4. The GABA antagonist bicuculline caused cell firing, mimicking the excitatory effect of DAMGO. Unlike DAMGO, however, bicuculline depolarized PAG cells. 5. Under voltage-clamp conditions, with the same driving force, the evoked inhibitory postsynaptic currents (IPSCs) in these neurons were 2.3 times larger than the evoked excitatory postsynaptic currents (EPSCs). Furthermore, DAMGO inhibited IPSCs by 60.7% while it inhibited EPSCs by 35.3%. 6. We propose that the overall effect of an opioid depends on the dynamic balance of its excitatory and inhibitory actions. In the PAG, the blockade of the inhibitory drive of GABAergic inputs by DAMGO is large. It overcomes the DAMGO-induced hyperpolarization and inhibition of EPSCs and thus results in the excitation of these neurons.
Preclinical Pharmacologic Evaluation of Pralatrexate and Romidepsin Confirms Potent Synergy of the Combination in a Murine Model of Human T-cell Lymphoma (2015)

Collectively, these data strongly suggest that the combination of pralatrexate and romidepsin merits clinical study in patients with TCLs.
Entry points into a smart campus environment - overview of the ETHOC system (2004)

The university campus is an interesting application environment for the ubiquitous computing paradigm: a large number of users share a substantial amount of their information needs and much of this information is directly related to physical objects, places, and people that are situated in the campus environment. We motivate the need for material entry points into a virtual campus that increase its accessibility and visibility from within the physical campus environment. We focus on the aspect of linking virtual and physical elements in such a setting and present the ETHOC system, which enables users to attach virtual counterparts to printed material. The system performs the creation, administration, and intermediation of online resources related to paper documents. To information providers, it offers a Web-based author portal for generating unique IDs that can be printed as barcodes and for associating online content and actions to printed material. To users it offers simple means to interact with virtual counterparts of printed documents using a variety of devices, such as WAP-enabled mobile phones or PDAs, and it stores a personal access history for each user. The ETHOC system allows us to put ubiquitous computing concepts into practical use and to gain new insights into the design of virtual counterparts of real-world objects.
Computationally Efficient Integration of Complex Thernal Multi-Chip Power Module Models into Circuit Simulators (2007)

For analyzing reliability or short-term overload conditions of power electronic systems, it is necessary to know transient temperatures of the power semiconductors. Directly coupling thermal and circuit simulators increases the simulation time by orders of magnitude, therefore making such an approach impractical. A well-known solution to this problem is to extract thermal equivalent circuits from 3D-field simulations and to insert them directly into the circuit simulator. In this paper we discuss the poor scaling performance of this state-of-the-art approach. There is an enormous increase in simulation time if there are more than just a few chips thermally modeled. We propose a general procedure at the circuit simulator solver level to increase the calculation speed of such a coupled simulation significantly.
The Selection of Classifiers for a Data-driven Parser (2015)

There is a large number of classifiers that can be used for generating a parse model; i.e., as an oracle for guiding data-driven parsers when parsing natural languages. In this paper we present a general and simple approach for generating a parse model. Additionally, we present a large number of experiments on various classifiers. We also present the effect of various parse models,\nwhich are generated from different classifiers, on a data-driven parser to see the way each model contributes to parsing performance.
Lloyd Interferometer Applied to Flatness Testing (1967)

Lloyd's mirror experiment is applied to testing flatness of large surfaces. Because of the grazing incidence, even rough surfaces provide the characteristic interference pattern. In the case of a perfectly flat surface, that pattern consists of narrowly and equally spaced two beam interference fringes. Departures from flatness are reflected in changes of the fringe spacing. Moiré techniques are used for visualizing and measuring these changes in the deviation from straightness of the Moiré fringes. Qualitative examples are given.
Notes - Cation Exchange Resins as Catalysts in the Alkylation of Phenols (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNotes - Cation Exchange Resins as Catalysts in the Alkylation of PhenolsBernard Loev and John MassengaleCite this: J. Org. Chem. 1957, 22, 8, 988–989Publication Date (Print):August 1, 1957Publication History Published online8 April 2003Published inissue 1 August 1957https://pubs.acs.org/doi/10.1021/jo01359a609https://doi.org/10.1021/jo01359a609research-articleACS PublicationsRequest reuse permissionsArticle Views180Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Gene Structure and cDNA Sequence Identify the Beaded Filament Protein CP49 as a Highly Divergent Type I Intermediate Filament Protein (1996)

The fiber cell of the vertebrate ocular lens assembles a cytoskeletal structure, the beaded filament, which contains two proteins unique to the fiber cell: CP49 (phakinin) and CP115/CP95 (filensin). We report here the complete primary sequence and gene structure for human CP49. These data show that CP49 is a member of the intermediate filament family, but highly unusual in several regards. 1) CP49 primary sequence does not permit unambiguous assignment to any existing class of intermediate filament protein, but exhibits a gene structure that is identical to the Type I cytokeratins. 2) CP49 essentially lacks one of the three major domains that characterize all intermediate filament proteins, the carboxyl-terminal tail domain. 3) CP49 shows substitutions at 3 of 4 residues in the otherwise highly conserved intermediate filament protein motif LNDR. Notably, this divergence includes an Arg to Cys substitution that has only been observed in the mutant human cytokeratin K14, a mutation shown to cause the skin blistering seen in the genetic disorder Dowling-Meara epidermolysis bullosa simplex.
The electrical behaviour of abrupt ion implanted and diffused P<sup>+</sup>N junctions (1971)

Abstract Results are reported of measurements of the properties of diodes formed by ion implantation, and for comparison boron diffused P+N diodes of similar area close by on the same chip. The four group III acceptor impurities were implanted separately to a dose of 5 × 1015 ions/cm2 at room temperature into similar samples of suitably masked silicon. Boron ions were also implanted at liquid nitrogen temperature and 450°C. Annealing was limited to a maximum temperatare of 550 °C. Measurements have been made of sheet resistance, forward and reverse I-V characteristics (from 10−9 amps/cm2), reverse breakdown voltage, noise, minority carrier storage time and junction series resistance. The bulk properties of boron implanted diodes were found to be reproducible. The introduction of recombination centres by implantation is the major factor influencing variation in these properties between one implantation condition and another. Changes in surface oxide conditions probably affect reverse leakage currents and breakdown voltages. The properties of boron implanted diodes are considered suitable for applications such as the MOSFET, and are superior to those of the AI, Ga and In implanted diodes.
Characterization of<i>Ferroplasma</i>Isolates and<i>Ferroplasma acidarmanus</i>sp. nov., Extreme Acidophiles from Acid Mine Drainage and Industrial Bioleaching Environments (2004)

Three recently isolated extremely acidophilic archaeal strains have been shown to be phylogenetically similar to Ferroplasma acidiphilum Y(T) by 16S rRNA gene sequencing. All four Ferroplasma isolates were capable of growing chemoorganotrophically on yeast extract or a range of sugars and chemomixotrophically on ferrous iron and yeast extract or sugars, and isolate "Ferroplasma acidarmanus" Fer1(T) required much higher levels of organic carbon. All four isolates were facultative anaerobes, coupling chemoorganotrophic growth on yeast extract to the reduction of ferric iron. The temperature optima for the four isolates were between 35 and 42 degrees C and the pH optima were 1.0 to 1.7, and "F. acidarmanus" Fer1(T) was capable of growing at pH 0. The optimum yeast extract concentration for "F. acidarmanus" Fer1(T) was higher than that for the other three isolates. Phenotypic results suggested that isolate "F. acidarmanus" Fer1(T) is of a different species than the other three strains, and 16S rRNA sequence data, DNA-DNA similarity values, and two-dimensional polyacrylamide gel electrophoresis protein profiles clearly showed that strains DR1, MT17, and Y(T) group as a single species. "F. acidarmanus" Fer1(T) groups separately, and we propose the new species "F. acidarmanus" Fer1(T) sp. nov.
Dual band MIMO antenna using ENG zeroth order resonator for 4G system (2009)

We propose a two-element diversity-antenna array using epsilon negative zeroth order resonator (ENG-ZOR) at the long-term evolution (LTE) and Wibro (WiMax in Korea) bands for the multiple-input multiple-output (MIMO) application. Each element is designed by utilizing an ENG ZOR unit cell with size of 10 mm × 6 mm × 6 mm. The lower resonant frequency is fixed by the zeroth order resonance of the ENG ZOR and the higher is selected by the radiator utilizing the first order resonance of the ENG ZOR. An isolator is added between the two elements in order to enhance the isolation characteristic at higher band of the designed MIMO antenna. The MIMO antenna is fabricated on the top side of the ground plane with size of 48 mm × 108 mm × 0.4 mm. The fabricated antenna has the isolation of about 20 dB at the lower band and higher than 13 dB at the higher band.
Distributed odor source localization (2002)

This paper presents an investigation of odor localization by groups of autonomous mobile robots. First, we describe a distributed algorithm by which groups of agents can solve the full odor localization task. Next, we establish that conducting polymer-based odor sensors possess the combination of speed and sensitivity necessary to enable real world odor plume tracing and we demonstrate that simple local position, odor, and flow information, tightly coupled with robot behavior, is sufficient to allow a robot to localize the source of an odor plume. Finally, we show that elementary communication among a group of agents can increase the efficiency of the odor localization system performance.
The lipocalin<i>α</i><sub>1</sub>-microglobulin protects erythroid K562 cells against oxidative damage induced by heme and reactive oxygen species (2008)

Alpha(1)-microglobulin is a 26 kDa plasma and tissue glycoprotein that belongs to the lipocalin protein superfamily. Recent reports show that it is a reductase and radical scavenger and that it binds heme and has heme-degrading properties. This study has investigated the protective effects of alpha(1)-microglobulin against oxidation by heme and reactive oxygen species in the human erythroid cell line, K562. The results show that alpha(1)-microglobulin prevents intracellular oxidation and up-regulation of heme oxygenase-1 induced by heme, hydrogen peroxide and Fenton reaction-generated hydroxyl radicals in the culture medium. It also reduces the cytosol of non-oxidized cells. Endogeneous expression of alpha(1)-microglobulin was up-regulated by these oxidants and silencing of the alpha(1)-microglobulin expression increased the cytosol oxidation. alpha(1)-microglobulin also inhibited cell death caused by heme and cleared cells from bound heme. Binding of heme to alpha(1)-microglobulin increased the radical reductase activity of the protein as compared to the apo-protein. Finally, alpha(1)-microglobulin was localized mainly at the cell surface both when administered exogeneously and in non-treated cells. The results suggest that alpha(1)-microglobulin is involved in the defence against oxidative cellular injury caused by haemoglobin and heme and that the protein may employ both heme-scavenging and one-electron reduction of radicals to achieve this.
Enzymes from solvent-tolerant microbes: Useful biocatalysts for non-aqueous enzymology (2009)

Solvent-tolerant microbes are a newly emerging class that possesses the unique ability to thrive in the presence of organic solvents. Their enzymes adapted to mediate cellular and metabolic processes in a solvent-rich environment and are logically stable in the presence of organic solvents. Enzyme catalysis in non-aqueous/low-water media is finding increasing applications for the synthesis of industrially important products, namely peptides, esters, and other trans-esterification products. Solvent stability, however, remains a prerequisite for employing enzymes in non-aqueous systems. Enzymes, in general, get inactivated or give very low rates of reaction in non-aqueous media. Thus, early efforts, and even some recent ones, have aimed at stabilization of enzymes in organic media by immobilization, surface modifications, mutagenesis, and protein engineering. Enzymes from solvent-tolerant microbes appear to be the choicest source for studying solvent-stable enzymes because of their unique ability to survive in the presence of a range of organic solvents. These bacteria circumvent the solvent's toxic effects by virtue of various adaptations, e.g. at the level of the cytoplasmic membrane, by degradation and transformation of solvents, and by active excretion of solvents. The recent screening of these exotic microbes has generated some naturally solvent-stable proteases, lipases, cholesterol oxidase, cholesterol esterase, cyclodextrin glucanotransferase, and other important enzymes. The unique properties of these novel biocatalysts have great potential for applications in non-aqueous enzymology for a range of industrial processes.
Improvement of an Unusual Twin-Arginine Transporter Leader Peptide by a Codon-Based Randomization Approach (2006)

Secretion of Escherichia coli penicillin acylase was improved by codon-based random mutagenesis of its signal peptide. The mutagenesis technology was applied to the gene region coding for positions Lys2 to Thr13 (N half) and Ala14 to Leu25 (C half) of the signal peptide. Protein secretion was higher in several signal peptide variants (up to fourfold with respect to the wild-type value).
Epidermal growth factor and transforming growth factor-?? actions on the gut (1995)

Epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) are part of a large family of ligands that bind to the epidermal growth factor receptor. While the precise role of these ligands is still unclear, there is little doubt that they play an important role in the development and maintenance of gastrointestinal epithelial continuity. The main ligand for the intestinal EGF receptor (EGFR) may in fact be TGF-alpha, and there is evidence that EGF itself may act as a surveillance factor in adults, only having an effect if the mucosa is damaged.