gebhart/patrepeval huggingface dataset:
Identification of Endogenous Phosphorylation Sites of Bovine Medium and Low Molecular Weight Neurofilament Proteins by Tandem Mass Spectrometry (2004)

Neurofilament proteins (NFP) are intermediate filaments found in the neuronal cytoskeleton. They are highly phosphorylated, a condition that is believed to be responsible for the assembly and stability of the filaments. Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) shows molecular masses for bovine NFP subunits of 63, 105, and 125 kDa for NFL, NFM, and NFH. Mass spectrometric de novo sequencing was used to determine the N-terminal sequence of bovine NFM (115 amino acids), which was previously unknown. Molecular mass information shows that there is one-half equivalent phosphate group on NFL and 24 on NFM. For the first time, it is shown that bovine NFL has three phosphorylation sites (Ser(55), Ser(66), and Ser(472)) and NFM has 22 (Ser(512), Ser(546), Ser(554), Ser(560), Thr(627), Ser(629), Ser(634), Ser(639), Thr(646), Ser(649), Ser(654), Ser(664), Ser(669), Thr(676), Ser(679), Ser(684), Ser(694), Ser(726), Ser(750), Ser(756), Ser(770), and Ser(846)) and two tentative sites (Ser(659)/Thr(661) and Thr(840)). Ser(66) was previously not known to be phosphorylated in NFL of other species, while two sites (Ser(55) and Ser(472)) are consistent with the phosphorylations observed in other mammalian NFLs. The three sites, Ser(55), Ser(66), Ser(472), are heterogeneously phosphorylated. Phosphorylation in bovine NFM occurs mainly in the Lys-Ser-Pro (KSP) region, but the Val-Ser-Pro and Ser-Glu-Lys motifs are also phosphorylated. Most of the phosphorylation sites are in accordance with those previously identified in other mammalian NFMs. In bovine NFM, 16 out of the 22 sites are always phosphorylated (Ser(512), Thr(627), Ser(629), Ser(634), Ser(639), Thr(646), Ser(649), Ser(654), Ser(664), Ser(669), Thr(676), Ser(679), Ser(684), Ser(694), Ser(726), and Ser(750)), all of which are contained in the KSP region, and six are sometimes phosphorylated (Ser(546), Ser(554), Ser(560), Ser(756), Ser(770), and Ser(846)). The NFPs have other modifications, including deamidation, oxidation, and N-terminal acetylation. Pyroglutamic acid formation also occurs.
Synthesis of an Optically Active, Bicyclic 2-Pyridone Dipeptide Mimetic (2002)

The eleven-step preparation of the bicyclic 2-pyridone dipeptide mimetic 1 [(3S)-6-(benzyloxycarbonylamino)-5-oxo-1,2,3,5-tetrahydroindolizine-3-carboxylic acid] in optically active form (60% ee) is described. Key steps in the synthesis of 1 include the osmium-catalyzed asymmetric dihydroxylation of olefin 13 [(6-but-3-enyl-2-methoxypyridin-3-yl)carbamic acid benzyl ester] and the intramolecular cyclization of protected diol 19 [(3'R)-[6-[4'-(tert-butyldimethylsilanyloxy)-3'-hydroxybutyl]-2-methoxypyridin-3-yl]carbamic acid benzyl ester] to afford the pyridinium salt 20 [(3S)-[3-(tert-butyldimethylsilanyloxymethyl)-5-methoxy-2,3-dihydro-1H-indolizin-6-yl]carbamic acid benzyl ester trifuoromethanesulfonic acid salt]. Several alternate methods to prepare olefin 13 are also discussed.
Mid-IR supercontinuum generation in Ho-doped fiber amplifier (2011)

For the first time to our best knowledge we have used Ho-doped fiber amplifier to get supercontinuum in the mid-IR range using all-fiber scheme. To pump the source Q-switched Er-doped fiber was used. The observed spectrum covers the spectral range from 2000 to 2500 nm with the power variation less than two decades. Average power of 0.4 W and pulse energy of 0.1 mJ was measured.
Distributed audit trail analysis (2002)

An implemented system for on-line analysis of multiple distributed data streams is presented. The system is conceptually universal since it does not rely on any particular platform feature and uses format adaptors to translate data streams into its own standard format. The system is as powerful as possible (from a theoretical standpoint) but still efficient enough for on-line analysis thanks to its novel rule-based language (RUSSEL) which is specifically designed for efficient processing of sequential unstructured data streams. The generic concepts are applied to security audit trail analysis. The resulting system provides powerful network security monitoring and sophisticated tools for intrusion/anomaly detection. The rule-based and command languages are described as well as the distributed architecture and the implementation. Performance measurements are reported, showing the effectiveness of the approach.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Separation Characteristics Of Dissolved Gases From Water Using A Polypropylene Hollow Fiber Membrane Module With High Surface Area (2014)

A polypropylene hollow fiber membrane module is used for separating dissolved gases which contain dissolved oxygen from water. These dissolved gases can be used for underwater breathing. To be used for a human, the minimum amount of oxygen is essential. To increase separation of dissolved gases, much water and high surface area of hollow fibers are requested. For efficient separation system, performance of single membrane module with high surface area needs to be investigated. In this study, we set up experimental devices for analyzing separation characteristics of dissolved gases including oxygen from water using a polypropylene hollow fiber membrane module. Separation of dissolved gases from water is investigated with variations of water flow rates. Composition of dissolved gases is also measured using GC. These results expect to be used in developing the portable separation system.
Low molecular weight GTP-binding proteins in human neutrophil granule membranes. (1991)

Degranulation of neutrophils involves the differential regulation of the exocytosis of at least two populations of granules. Low molecular weight GTP-binding proteins (LMW-GBPs) have been implicated in the regulation of vesicular traffic in the secretory pathways of several types of cells. In the present study we identify distinct subsets of LMW-GBPs associated with the membranes of neutrophil-specific and azurophilic granules. Ninety-four percent of total [35S]guanosine 5'-(3-O-thio)triphosphate (GTP gamma S) binding activity was equally distributed between the plasma membrane and cytosol with the remaining 6% localized in the granules. In contrast, the cytosol contained only 10% of the total GTPase activity while the specific granules accounted for 13%. [alpha-32P]GTP binding to proteins transferred to nitrocellulose revealed LMW-GBPs in all fractions except the azurophilic granules. The specific granules contained three out of four bands which were found in the plasma membrane; these ranged from 20 to 23 kDa and all were resistant to alkaline extraction. Photoaffinity labeling with [alpha-32P]8-azido-GTP in the presence of micromolar Al3+ identified proteins of 25 and 26 kDa unique to azurophilic granules; these could not be labeled with [alpha-32P]8-azido-ATP and could be extracted by acidic but not alkaline pH. Botulinum C3-mediated [32P]ADP-ribosylation identified proteins of 16, 20, and 24 kDa both in plasma membranes and those of specific granules. An anti-ras monoclonal antibody, 142-24E5, recognized a 20-kDa protein localized to the plasma and specific granule membranes which could not be extracted by alkaline pH, was not a substrate for botulinum C3 ADP-ribosyltransferase, and was translocated from specific granules to plasma membrane after exposure of neutrophils to phorbol myristate acetate. We conclude that neutrophil-specific and azurophilic granules contain distinct subsets of LMW-GBPs which are uniquely situated to regulate the differential exocytosis of these two compartments.
[Problems of congenital toxoplasmosis. Evolution over four decades]. (1999)

EFFECT OF PREVENTIVE MEASURES: The development of reliable routine serology tests and the demonstration of the high prevalence of toxoplasmosis in France led to mandatory prospective screening of pregnant women in 1978, followed by prenatal screening in 1985. In addition, in utero diagnosis, first on fetal blood and now with the reliable and safe method using polymerase chain reaction on amniotic fluid, formally identifies the parasite. THE SITUATION TODAY: A comparison of the experience of a specialized center in Paris during three different periods over the last 40 years showed that currently 71% of all cases of congenital toxoplasmosis are infraclinical at birth and only 5% are severe. Mean incidence of seroconversion during pregnancy is 1.48%, with a 40% risk of fetal contamination if no treatment is given. The risk of overt fetopathy predominantly concerns maternal infections occurring prior to 26 weeks gestation. IN UTERO TREATMENT: Positive PCR diagnosis on amniotic fluid imposes serial ultrasound examinations to identify any fetopathy and an in utero treatment by giving the mother the pyrimethamine-sulfadiazine combination. Biological results are favorable. POST-NATAL TREATMENT: Post-natal treatment is indicated even in latent forms and should be continued for the first year of life. The pyrimethamine-sulfadiazine combination is the only pharmaceutical regimen with well-proven efficacy. New compounds should allow better prophylaxis against maternofetal contamination or improved post-natal treatment. RECURRENCE: In 70% of the cases, serology tests become positive again, but are not associated with significantly increased risk of ocular recurrence.
Imatinib mesylate therapy for relapse after allogeneic stem cell transplantation for chronic myelogenous leukemia (2002)

Twenty-eight adults with chronic myelogenous leukemia (CML) that had relapsed after allogeneic stem cell transplantation (SCT) received imatinib mesylate (400-1000 mg/d). Disease was in chronic phase in 5 patients, accelerated in 15, and blastic in 8 (7 medullary, 1 extramedullary); median time from transplantation to relapse was 9 months (range, 1-137 months). Thirteen patients had undergone salvage donor lymphocyte infusion (DLI) (median time from DLI to imatinib mesylate therapy, 4 months [range, 2-39 months]). The overall response rate was 79% (22 of 28 patients); the complete hematologic response (CHR) rate was 74% (17 of 23 patients), and the cytogenetic response rate was 58% (15 of 26 patients; complete response in 9 [35%] patients). CHR rates were 100% for chronic phase, 83% for accelerated phase, and 43% for blastic phase. The patient with extramedullary blastic disease achieved complete response. Cytogenetic response rates were 63% (12 of 19 patients) for chronic or accelerated phases (complete cytogenetic response in 8) and 43% for blastic phase (3 of 7 patients). At median follow-up of 15 months, 19 patients were alive, 9 with no evidence of disease. The 1-year estimated survival rate was 74%. Five patients had recurrence of grade 3 (3 patients) or grades 1 to 2 (2 patients) graft-versus-host disease (GVHD). Severe granulocytopenia developed in 43% of patients and thrombocytopenia in 27%; both conditions reversed with dose adjustments of imatinib mesylate. We conclude that imatinib mesylate effectively controlled CML that recurred after allogeneic SCT, but it was associated with side effects including myelosuppression and recurrence of severe GVHD.
Characterization of Plasma‐Polymerized Thiophene Thin Films and Nanoparticles Synthesized by a Double‐Discharge Technique (2008)

Abstract PTH nanoparticles and thin films were synthesized via a plasma polymerization technique using a double‐discharge system. This system has a fast filamentary discharge formed from the superposition of an ordinary low‐pressure dc glow discharge and a high‐current pulsed one. The samples were characterized by SEM, TEM, XPS, UV‐vis and FT‐IR spectroscopy. We found that superposing two discharges allows to control the morphology of the films. In the high‐temperature region, smooth and uniform thin films were produced, whereas gas‐phase polymerization resulted in films with grains of irregular shape. However, the films produced in the gas‐phase region exhibited highly thiophenic structures and could be dispersed in DCM, giving nanoparticles of 30 to 50 nm diameter. magnified image
Protective effects of sindacon on renal ischemic-reperfusion injury in rats (2011)

Aim:To investigate the protective effects of sindacon on renal ischemic-reperfusion injury(IRI)in rats.Methods:Fifty rats were randomly divided in five groups:sham operation group,model group,sindacon high,middle,low dosage groups(200,100,50 mg/kg).The renal IRI model was established after intragastric administration of corresponding drugs for seven days among sindacon high,middle,low dosage groups.Rats of model group were administrated saline.The scores of renal tubule injury were assessed.The levels of blood urea nitrogen(BUN)and creatinine(Cr)in serum,activity of superoxidase(SOD)and content of malondialdehyde(MDA)in renal homogenate were measured.Results:The scores of renal tubule injury,and the levels of blood BUN and Cr,SOD and MDA in renal homogenate among the five groups were obviously different(F were 136.710,102.695,99.754,16.471,71.333,P0.001).Compared with the model group,the scores of renal tubule injury,the levels of BUN and Cr were higher and the level of MDA was lower in sindacon high,and middle dosage groups.The levels of SOD in sindacon high grouphigher than that in model group(P0.05).Conclusion:Sindacon has protective effect in rats on renal IRI,and the mechanism may be due to its antioxidant capacity.
The effectiveness of an AR-based context-aware assembly support system in object assembly (2014)

This study evaluates the effectiveness of an AR-based context-aware assembly support system with AR visualization modes proposed in object assembly. Although many AR-based assembly support systems have been proposed, few keep track of the assembly status in real-time and automatically recognize error and completion states at each step. Naturally, the effectiveness of such context-aware systems remains unexplored. Our test-bed system displays guidance information and error detection information corresponding to the recognized assembly status in the context of building block (LEGO) assembly. A user wearing a head mounted display (HMD) can intuitively build a building block structure on a table by visually confirming correct and incorrect blocks and locating where to attach new blocks. We proposed two AR visualization modes, one of them that displays guidance information directly overlaid on the physical model, and another one in which guidance information is rendered on a virtual model adjacent to the real model. An evaluation was conducted to comparatively evaluate these AR visualization modes as well as determine the effectiveness of context-aware error detection. Our experimental results indicate the visualization mode that shows target status next to real objects of concern outperforms the traditional direct overlay under moderate registration accuracy and marker-based tracking.
The impact of mentoring and collegial support on faculty success: An analysis of support behavior, information adequacy, and communication apprehension (1989)

This study investigates the relationship between mentoring and collegial support systems, information adequacy, communication apprehension, and faculty success. Professors (N = 224) at two universities were surveyed. Results showed that patterns of communication and success differed by gender/mentee status. Canonical analysis, controlling for gender/mentee status, supported the hypothesized relationships.
K‐band CMOS frequency doubler with high fundamental rejection (2014)

The design of a frequency doubler with current‐reuse and fundamental rejection topology is presented. The frequency doubler is fabricated by a standard 0.18 μm CMOS process with a chip size of 0.57 mm 2 . The DC power consumption is 13.9 mW. In addition, the measured conversion gain is between –4.4 and –5.24 dB from 10 to 12 GHz input frequency. With the proposed active notch filter in the cascode stage of the doubler, a superior fundamental rejection is obtained. The measured fundamental rejection is between 32.4 and 53.8 dB.
Complete assignments of <sup>1</sup>H and <sup>13</sup>C NMR data for three new arylnaphthalene lignan from <i>Justicia procumbens</i> (2008)

Three new arylnaphthalene lignans, named neojusticin C (1), procumbenoside C (2) and procumbenoside D (3), have been isolated from the whole plant of Justicia procumbens, together with three known ones, justicidinoside B (4), justicidinoside C (5), and diphyllin-1-O-beta-D-apiofuranoside (6). The complete assignments of 1H and 13C NMR data for three new lignans were first obtained by means of 2D NMR techniques, including HSQC and HMBC.
Severed corticospinal axons recover electrophysiologic control of muscle activity after x-ray therapy in lesioned adult spinal cord. (1996)

Mechanical injury to the adult mammalian spinal cord results in permanent loss of structural integrity at the lesion site and of the brain-controlled function distal to the lesion. Some of these consequences were permanently averted by altering the cellular constituents at the lesion site with x-irradiation delivered within a critical time window after injury. We have reported in a separate article that x-irradiation of sectioned adult rat spinal cord resulted in restitution of structural continuity and regrowth of severed corticospinal axons across and deep into the distal stump. Here, we report that after x-ray therapy of the lesion site severed corticospinal axons of transected adult rat spinal cord recover electrophysiologic control of activity of hindlimb muscles innervated by motoneurons distal to the lesion. The degree of recovery of control of muscle activity was directly related to the degree of restitution of structural integrity. This restitution of electrophysiologic function implies that the regenerating corticospinal axons reestablish connectivity with neurons within the target field in the distal stump. Our data suggest that recovery of structural continuity is a sufficient condition for the axotomized corticospinal neurons to regain some of their disrupted function in cord regions distal to the lesion site.
Design of a Suspended Stripline Narrow Bandpass Filter with Ultrawideband Harmonic Suppression (2018)

A design method of narrow bandpass filters (NBPFs) of 4–6% bandwidth with ultrawideband suppression of harmonic passbands, utilizing two cascaded step impedance resonators (SIRs) in a suspended stripline, is proposed in this paper. The proposed design utilized the characteristics of a suspended stripline, which provides a much higher characteristic impedance ratio as compared with that of the microstripline, enabling ultrawideband harmonic suppression. As an example of the NBPF, a filter with a passband center frequency <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:msub><mml:mrow><mml:mi>f</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:math> of 0.75 GHz and bandwidth of 5% was implemented and proved to suppress the harmonic passbands up to <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mn>13.5</mml:mn><mml:mtext> </mml:mtext><mml:msub><mml:mrow><mml:mi>f</mml:mi></mml:mrow><mml:mrow><mml:mn>0</mml:mn></mml:mrow></mml:msub></mml:math>. Since the proposed filter was implemented on the suspended stripline, the passband insertion loss was only −0.9 dB, which is low as compared with other previous designs. The proposed filter is a compact high-performance low-loss NBPF, which can be applicable to various wireless systems.
Dynamic optical tomographic imaging of the human forearm for quantification of the vascular bed response in diabetics and non-diabetics (2004)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text N. A. Franco, R. E. Hardin, D. P. Klemer, M. S. Katz, H. L. Graber, C. H. Schmitz, R. L. Barbour, A. G. Smeraldi, and T. F. Panetta, "Dynamic optical tomographic imaging of the human forearm for quantification of the vascular bed response in diabetics and non-diabetics," in Biomedical Topical Meeting, OSA Technical Digest (Optica Publishing Group, 2004), paper WF37. Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
The role of tumor-associated macrophages in tumor vascularization (2013)

Tumor vascularization is a highly complex process that involves the interaction between tumors and their surrounding stroma, as well as many distinct angiogenesis-regulating factors. Tumor associated macrophages (TAMs) represent one of the most abundant cell components in the tumor environment and key contributors to cancer-related inflammation. A large body of evidence supports the notion that TAMs play a critical role in promoting the formation of an abnormal tumor vascular network and subsequent tumor progression and invasion. Clinical and experimental evidence has shown that high levels of infiltrating TAMs are associated with poor patient prognosis and tumor resistance to therapies. In addition to stimulating angiogenesis during tumor growth, TAMs enhance tumor revascularization in response to cytotoxic therapy (e.g., radiotherapy), thereby causing cancer relapse. In this review, we highlight the emerging data related to the phenotype and polarization of TAMs in the tumor microenvironment, as well as the underlying mechanisms of macrophage function in the regulation of the angiogenic switch and tumor vascularization. Additionally, we discuss the potential of targeting pro-angiogenic TAMs, or reprograming TAMs toward a tumoricidal and angiostatic phenotype, to promote normalization of the tumor vasculature to enhance the outcome of cancer therapies.
Decreased expression of Drp1 and Fis1 mediates mitochondrial elongation in senescent cells and enhances resistance to oxidative stress through PINK1 (2010)

Mitochondria display different morphologies, depending on cell type and physiological situation. In many senescent cell types, an extensive elongation of mitochondria occurs, implying that the increase of mitochondrial length in senescence could have a functional role. To test this hypothesis, human endothelial cells (HUVECs) were aged in vitro. Young HUVECs had tubular mitochondria, whereas senescent cells were characterized by long interconnected mitochondria. The change in mitochondrial morphology was caused by downregulation of the expression of Fis1 and Drp1, two proteins regulating mitochondrial fission. Targeted photodamage of mitochondria induced the formation of reactive oxygen species (ROS), which triggered mitochondrial fragmentation and loss of membrane potential in young cells, whereas senescent cells proved to be resistant. Alterations of the Fis1 and Drp1 expression levels also influenced the expression of the putative serine-threonine kinase PINK1, which is associated with the PARK6 variant of Parkinson's disease. Downregulation of PINK1 or overexpression of a PINK1 mutant (G309D) increased the sensitivity against ROS in young cells. These results indicate that there is a Drp1- and Fis1-induced, and PINK1-mediated protection mechanism in senescent cells, which, when compromised, could contribute to the age-related progression of Parkinson's disease and arteriosclerosis.