gebhart/patrepeval huggingface dataset:
How Close Is Close Enough?: Testing Nonexperimental Estimates Of Impact Against Experimental Estimates Of Impact With Education Test Scores As Outcomes (2002)

In this study we test the performance of some nonexperimental estimators of impacts applied to an educational intervention—reduction in class size—where achievement test scores were the outcome. We compare the nonexperimental estimates of the impacts to "true impact" estimates provided by a random-assignment design used to assess the effects of that intervention. Our primary focus in this study is on a nonexperimental estimator based on a complex procedure called propensity score matching. Previous studies which tested nonexperimental estimators against experimental ones all had employment or welfare use as the outcome variable. We tried to determine whether the conclusions from those studies about the performance of nonexperimental estimators carried over into the education domain. Project Star is the source of data for the experimental estimates and the source for drawing nonexperimental comparison groups used to make nonexperimental estimates. Project Star was an experiment in Tennessee involving 79 schools in which students in kindergarten through third grade were randomly assigned to small classes (the treatment group) or to regular-size classes (the control group). The outcome variables from the data set were the math and reading achievement test scores. We carried out the propensity-score-matching estimating procedure separately for each of 11 schools' kindergartens and used it to derive nonexperimental estimates of the impact of smaller class size. We also developed proper standard errors for the propensity-score-matched estimators by using bootstrapping procedures. We found that in most cases, the propensity-score estimate of the impact differed substantially from the "true impact" estimated by the experiment. We then attempted to assess how close the nonexperimental estimates were to the experimental ones. We suggested several different ways of attempting to assess "closeness." Most of them led to the conclusion, in our view, that the nonexperimental estimates were not very "close" and therefore were not reliable guides as to what the "true impact" was. We put greatest emphasis on looking at the question of "how close is close enough" in terms of a decision-maker trying to use the evaluation to determine whether to invest in wider application of the intervention being assessed—in this case, reduction in class size. We illustrate this in terms of a rough cost-benefit framework for small class size as applied to Project Star. We find that in 30 to 45 percent of the 11 cases, the propensity-score-matching nonexperimental estimators would have led to the "wrong" decision.
Mapping of Functional Regions in the Amino-Terminal Portion of the Herpes Simplex Virus ICP27 Regulatory Protein: Importance of the Leucine-Rich Nuclear Export Signal and RGG Box RNA-Binding Domain (2002)

Infected-cell protein 27 (ICP27) is an essential herpes simplex virus type 1 (HSV-1) regulatory protein that activates a subset of viral delayed-early and late genes, at least in part through posttranscriptional mechanisms. Previous studies have shown that the amino (N)-terminal half of the protein contains important functional regions, including a leucine-rich nuclear export signal (NES). However, to date, the phenotype of an HSV-1 ICP27 NES mutant has not been reported. In this study, we engineered and characterized dLeu, an HSV-1 deletion mutant that specifically lacks ICP27's NES (amino acids 6 to 19). The phenotype of dLeu was analyzed alongside those of eight other ICP27 N-terminal deletion mutants. We found that in Vero cells, dLeu displays modest defects in viral gene expression and an approximately 100-fold reduction in the production of viral progeny. Unlike wild-type (WT) ICP27, which exhibits a cytoplasmic distribution in addition to its predominant nuclear localization, dLeu ICP27 is highly restricted to the cell nucleus. This strongly suggests that the N-terminal leucine-rich sequence functions as an NES during viral infection. Our analysis of dLeu and the other mutants has enabled us to genetically define the regions in the N-terminal 200 residues of ICP27 which are required for efficient viral growth in Vero cells. Only two regions appear to be important: (i) the leucine-rich NES and (ii) the RGG box RNA-binding domain, encoded by residues 139 to 153. A virus lacking the RGG box-encoding sequence, d4-5, has a phenotype similar to that of dLeu in that it displays modest defects in viral gene expression and grows poorly. Interestingly, deletion of both the NES and RGG box, as well as the sequences in between, is lethal. The resulting virus, d1-5, displays severe defects in viral gene expression and DNA synthesis and is unable to produce significant amounts of infectious progeny. Therefore, the N-terminal portion of ICP27 contains at least two functional domains which collectively are absolutely essential for viral infection.
Clinical Considerations of Dimethyl Sulfoxide (1984)

Dimethyl sulfoxide (DMSO) has been heralded as a "miracle" drug capable of a wide range of therapeutic capabilities. While its biological actions are indeed diverse, one must reserve judgement on the attributes of DMSO until further clinical studies involving the specific actions of DMSO are conducted. It is clear that DMSO has amazing properties that certainly could be useful in the treatment of many medical disorders.
원저 : 피부 백혈병의 임상 및 병리조직학적 연구 (1990)

A clinicopathological study was made on 17 case with leukemia cutis diagnosed in a period of 10 years frorn 1980 to 1989 in Seoul Xational University I-lospital. The results were summarized as follows : 1. There were 4 cases with acute lyrnphocytic leukernia, 
7 with acute myelocytir. leukemia (AML), 5 with chronic myelocytic leukemia (CML), 1 with eosinophilic leukemia. 2. The clinical appearance of leukemia cutis included nodules (88.2%), papules, macules, plaques and ulcerative lesions. Leukemia cutis dis not show any discernible clinical qppearance with each different type of leukemia. 3. Leukemia cutis showed a wide spectrum of histopathologic features, diffuse infiltration, patchy 
infiltration with linear infiltration between collagen bundles, perivascular and peridnexal involvement, perivascular involvement, and mainly subcutaneous tissue involvement. There was no distinctive histopathologic pattern with each different type of leukemia. 4. The demonstration of intracytoplasmic chloroacetate esterase and lysozyme was helpful 
to refine the diagnosis of leukemia cutis. 
(Kor J Dermatol 28(3): 321 330, 1990)
Dynamic three-dimensional reconstruction of ICUS images based on an EGG-gated pull-back device (2002)

At present most systems used for three-dimensional reconstruction (3-D) of two-dimensional intracoronary ultrasound (ICUS) images are based on an image acquisition with a pull-back device which withdraws the catheter with a constant speed, not taking account of cardiac motion and coronary dynamic/pulsation. Cyclic changes of the vessel dimensions and the movement of the catheter inside the vessel result in artifacts and inaccuracies of quantitative measurements. This phenomenon limits accuracy and resolution when an attempt of 3-D reconstruction is performed, since images obtained in different phases of the cardiac cycle are compiled. To overcome these limitations the authors developed a custom-designed pull-back device driven by a stepping motor, which is controlled by a steering logic ensuring an ECG-gated image acquisition.
Role of pRb-Related Proteins in Simian Virus 40 Large-T-Antigen-Mediated Transformation (1995)

Simian virus 40 large T-antigen (TAg) transformation is thought to be mediated, at least in part, by binding to and modulating the function of certain cellular proteins, including the retinoblastoma tumor suppressor gene product, pRb. TAg can disrupt the inhibitory complexes formed by pRb with the oncogenic transcription factor E2F, and this mechanism has been suggested to be important for TAg-mediated transformation. Residues 102 to 114 of TAg (including the LXCXE motif) are required for binding to pRb. Mutations within this LXCXE motif abolish the ability of TAg to bind to pRb as well as to transform certain cell types. TAg can also bind to at least two other cellular proteins, p107 and p130, that are related to pRb by sequence homology and share the ability to bind E2F. However, whether p107 and p130 are also targets in TAg-mediated transformation is less clear. To assess the relative contribution of the inactivation of pRb, p107, and p130 to transformation by TAg, fibroblasts were prepared from embryos derived from matings of mice heterozygous for an Rb knockout allele. The ability of TAg to transform fibroblasts homozygous for either wild-type or knockout Rb alleles was evaluated. It is demonstrated that the integrity of the LXCXE motif provides a growth advantage in Rb+/+ and Rb-/- cells. Furthermore, wild-type TAg, but not the LXCXE mutants, could bind to p107 and p130 and disrupt p107-E2F and p130-E2F binding complexes. These results suggest that p107 and p130 participate in TAg-mediated transformation and that they may behave as tumor suppressors.
Molecular Genetics of Human B- and T-cell Neoplasia (1986)

More than 95% of human hematopoietic malignancies carry nonrandom chromosomal alterations, predominantly chromosomal translocations, inversions, deletions, and trisomies (Yunis 1983). During the past 5 years, it has become clear that such specific chromosomal rearrangements are not epiphenomena, but instead are directly involved in the pathogenesis of human leukemias and lymphomas (Croce and Nowell 1985). It seems likely that chromosomal alterations also play a very important role in the pathogenesis of human solid tumors, since recent studies by standard and high-resolution banding techniques indicate that more than 90% of these tumors contain clonal cytogenetic changes (Yunis 1983). Unfortunately, with very few exceptions, such as Ewing sarcomas and synovial sarcomas, it has not yet been possible to correlate specific chromosomal rearrangements with specific human solid tumors. One possible reason for this is that cells of solid tumors carry numerous chromosomal alterations, making it very difficult to determine whether these are consistent cytogenetic...
Serine-Arginine-rich Protein p30 Directs Alternative Splicing of Glucocorticoid Receptor Pre-mRNA to Glucocorticoid Receptor β in Neutrophils (2003)

Glucocorticoid (GC) insensitivity is a major clinical challenge in the treatment of many inflammatory diseases. It has been shown previously that GC insensitivity, in several inflammatory cell types, is due to an overabundance of the beta isoform of the glucocorticoid receptor (GCRbeta) relative to the ligand binding isoform, GCRalpha. GCRbeta functions as a dominant inhibitor of GCRalpha action. A number of GCR isoforms are created from the same pre-mRNA transcript via alternative splicing, and the factor or factors that control alternative splicing of GCR pre-mRNA are of great importance. In the current study, we have identified the predominant alternative splicing factor present in human neutrophils, which is known to be exceptionally GC-insensitive. The predominant alternative splicing factor in neutrophils is SRp30c, which is one of several highly conserved serine-arginine-rich (SR) proteins that are involved in both constitutive and alternative splicing in eukaryotic cells. Inhibition of SRp30c expression with antisense oligonucleotide strongly inhibited expression of GCRbeta and stimulated expression of GCRalpha. Antisense molecules targeted to other SR proteins had no effect. Our data indicate that SRp30c is necessary for alternative splicing of the GCR pre-mRNA to create mRNA encoding GCRbeta.
Macro to nano: a simple method for transporting cultured cells from milliliter scale to nanoliter scale (2010)

Microfluidic devices are well-suited for the study of metabolism and paracrine and autocrine signaling because they allow steady or intermittent perfusion of biological cells at cell densities that approach those in living tissue. They also enable the study of small populations of rare cells. However, it can be difficult to introduce the cells into a microfluidic device to achieve and control such densities without damaging or clumping the cells. We describe simple procedures that address the problem of efficient introduction of cells and cell culture media into microfluidic devices using small bore polyetheretherketone (PEEK) tubing and Hamilton gastight syringes. Suspension or adherent cells grown in cell culture flasks are centrifuged and extracted directly from the centrifuge pellet into the end of the PEEK tubing by aspiration. The tube end is then coupled to prepunched channels in the polydimethylsiloxane microfluidic device by friction fitting. Controlled depression of the syringe plunger expels the cells into the microfluidic device only seconds following aspiration. The gastight syringes and PEEK tubing with PEEK fittings provide a non-compliant source of pressure and suction with a rapid response time that is well suited for short-term intramicrofluidic cellular studies. The benefits of this method are its simplicity, modest expense, the short preparation time required for loading appropriate numbers of cells and the applicability of the technique to small quantities of rare or expensive cells. This should in turn lead to new applications of microfluidic devices to biology and medicine.
Polymorphism of UDP-Glucuronosyltransferase and Drug Metabolism (2005)

UDP-glucuronosyltransferase is a group of catabolic enzymes involved in the detoxification and excretion of many xenobiotic and endogeneous substances in intrahepatic and extrahepatic tissues. The group consists of two subfamilies, UGT1 and UGT2. UGT1 consists of 5 exons and has a unique gene structure. There are thirteen exon 1s from UGT1A1 to UGT1A13P, and exon 2 to exon 5 are used in common for all mRNAs expressed from the gene. Each isoform of UGT1 results from differential splicing of exon1s to common exon 2-5, and has an unique spectrum of substrate specificity. In contrast, the genes of the UGT2 family consist of 6 exons, and all the enzymes have an individual set of exon 1 to exon 6. In UGT1 there are no reports of polymorphism in the common exons, although a number of polymorphisms have been reported for exon 1s. The mutations of UGT1A1 cause hereditary unconjugated hyperbilirubinemias: Crigler-Najjar syndrome type I, type II and Gilbert syndrome. UGT1A1 has two major polymorphisms--a missense mutation of G71R and an insertion mutation of TATA box. Prevalence of Gilbert syndrome is attributed to these polymorphisms. Since UGT1A1 metabolizes not only bilirubin but also hormones and drugs, the mutations could be involved in carcinogenesis and adverse drug reactions. Recent studies also revealed a widespread presence of diverse polymorphisms in other isoforms of UGT1 as well as the UGT2 family, including UGT1A6, UGTG1A7, UGT1A8, UGT1A10, UGT2B4, UGT2B7 and UGT2B15. The incidences and types of the polymorphisms for these enzymes are quite different in region and ethnic groups. Understanding of these polymorphisms is essential for the prevention of adverse effects of a considerable number of drugs and to predict cancer risks.
Transcriptional organization of the rfaGBIJ locus of Salmonella typhimurium (1991)

The transcriptional organization of the rfaGBIJ gene cluster of Salmonella typhimurium was studied by using lacZ and cat transcriptional probes. The results indicated that the leftward end of the gene cluster (rfaG-rfaB-rfaI) is an operon that is transcribed from one or more promoters that lie upstream of rfaG. The results further indicated that the product of the rfaH (sfrB) gene acts as a positive regulator of transcription of the entire rfaGBIJ cluster. At least one site required for the RfaH-mediated transcriptional regulation lies within or very close to the upstream promoter.
DETECTION OF SOCCER GOAL SHOTS USING JOINT MULTIMEDIA FEATURES AND CLASSIFICATION RULES (2003)

Hyaluronan (sodium hyaluronate) is a glycosaminoglycan that is present in all joint tissues. Painful arthritic joints have been characterized by hyaluronan of reduced elastoviscosity. The purpose of this investigation was to determine whether hyaluronan has an influence on joint nociceptor sensitivity and whether restoration of elastoviscosity would decrease nerve responses from nociceptive afferent fibers in arthritic joints. Nerve impulse activity was recorded from nociceptive afferent fibers of the medial articular nerve in anesthetized cats. An acute experimental arthritis was produced by intra-articular injection of kaolin and carrageenan. This caused, within 3 h, the development of ongoing nerve activity and enhancement of nerve impulse responses to passive movements in the normal range of the joint. Intra-articular injection of an elastoviscous solution of hylan, a hyaluronan derivative, significantly reduced both the ongoing activity and the movement-evoked responses in 1-2 h. This effect was not obtained when a nonelastoviscous solution of hylan was injected into the inflamed joint. The results indicate that intra-articularly injected elastoviscous solutions of hylan reduced nociceptive activity in inflamed joints through an elastoviscous, rheological effect on nociceptive afferent fibers through the intercellular matrix in which these fibers are embedded.
Safe Data: Recognising the Issue (2011)

This article explains that like software, data affects the behaviour of many systems, and has the potential to cause unsafe behaviour without the presence of any failure of the system hardware. However, while software is often designed as an integral part of a system, data may not even be recognised as forming a part of the system. It may also be generated by third parties considerably detached from the main system design effort. While systems can be designed to tolerate problems with the data they use, this requires designers to have recognised the contribution of data to safety. A taxonomy of potential data faults is proposed to aid analysis of how problems with data could contribute to unsafe system-level behaviour.
Free-radical cyclization of bromo acetals. Use in the construction of bicyclic acetals and lactones (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFree-radical cyclization of bromo acetals. Use in the construction of bicyclic acetals and lactonesGilbert Stork, Robert Mook Jr., Scott A. Biller, and Scott D. RychnovskyCite this: J. Am. Chem. Soc. 1983, 105, 11, 3741–3742Publication Date (Print):June 1, 1983Publication History Published online1 May 2002Published inissue 1 June 1983https://pubs.acs.org/doi/10.1021/ja00349a082https://doi.org/10.1021/ja00349a082research-articleACS PublicationsRequest reuse permissionsArticle Views2374Altmetric-Citations259LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The Use of Benzylsulfonyl Chloride in Peptide Syntheses<sup>1,2</sup> (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Use of Benzylsulfonyl Chloride in Peptide Syntheses1,2H. Bayard Milne and Chi-Hsieh PengCite this: J. Am. Chem. Soc. 1957, 79, 3, 639–644Publication Date (Print):February 1, 1957Publication History Published online1 May 2002Published inissue 1 February 1957https://pubs.acs.org/doi/10.1021/ja01560a036https://doi.org/10.1021/ja01560a036research-articleACS PublicationsRequest reuse permissionsArticle Views357Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Comparative Assessment of Patrons' Uses and Evaluations across Public Libraries within a System: A Replication. (1987)

Etude comparative du comportement des utilisateurs dans les 20 bibliotheques d'un reseau regional de bibliotheques, qui tend a prouver que les bibliotheques avec une quantite de sources differentes repondent differemment aux besoins des utilisateurs. Cette enquete essaie de definir les motivations des utilisateurs quant au choix de la bibliotheque
Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the Peyer's patches. (1994)

Salmonella species are known to initiate infection of mammalian hosts by penetrating the intestinal epithelium of the small bowel. These bacteria preferentially interact with Peyer's patches which are collections of lymphoid follicles making up the gut-associated lymphoid tissue. We infected murine ligated intestinal loops with invasive and noninvasive Salmonella typhimurium strains for 30, 60, 120, and 180 min and examined the infected tissue by transmission electron microscopy. Within 30 min, we found that invasive S. typhimurium exclusively entered M cells found within the follicle-associated epithelium (FAE) of the Peyer's patches. Initially, interactions between invasive bacteria and enterocytes adjacent to the M cells were not found. Invasion of M cells was associated with the ability of the bacteria to invade tissue culture cells. S. typhimurium mutants, which were noninvasive for tissue culture cells, could not be found in ligated loops associated with M cells or enterocytes after incubations of 30, 60, 120, or 180 min. At 60 min, internalized invasive S. typhimurium were cytotoxic for the M cells. Destruction of an M cell formed a gap in the FAE which allowed organisms to invade enterocytes adjacent to the dead cell. Later in the infection process (120 and 180 min), the presence of bacteria beneath the FAE correlated with changes in the cytoarchitecture of the lymphoid follicle. In addition, replicating Salmonella began to enter both the apical and basolateral surfaces of enterocytes adjacent to infected M cells.