gebhart/patrepeval huggingface dataset:
The Combination of Spontaneous Resolution and Asymmetric Catalysis: A Model for the Generation of Optical Activity from a Fully Racemic System (2001)

Enantiomerically pure BIPHOS was obtained by spontaneous resolution through the crystallization of a racemic mixture, without the use of chemical or physical chiral auxiliaries, and was used in the synthesis of the enantiomerically pure complex [PdCl2(biphos)] (see scheme). The complex proved to be efficient in catalytic asymmetric allylic substitutions with 80 % ee.
Mannose 6-Phosphate Receptor and Sortilin Mediated Endocytosis of α-Galactosidase A in Kidney Endothelial Cells (2012)

Prominent vasculopathy in Fabry disease patients is caused by excessive intracellular accumulation of globotriaosylceramide (GL-3) throughout the vascular endothelial cells causing progressive cerebrovascular, cardiac and renal impairments. The vascular lesions lead to myocardial ischemia, atherogenesis, stroke, aneurysm, thrombosis, and nephropathy. Hence, injury to the endothelial cells in the kidney is a key mechanism in human glomerular disease and endothelial cell repair is an important therapeutic target. We investigated the mechanism of uptake of α-galactosidase A (α-Gal A) in renal endothelial cells, in order to clarify if the recombinant enzyme is targeted to the lysosomes via the universal mannose 6-phosphate receptor (M6PR) and possibly other receptors. Immunohistochemical localization of infused recombinant α-Gal A in a renal biopsy from a classic Fabry disease patient showed that recombinant protein localize in the endothelial cells of the kidney. Affinity purification studies using α-Gal A resins identified M6PR and sortilin as α-Gal A receptors in cultured glomerular endothelial cells. Immunohistochemical analyses of normal human kidney with anti-sortilin and anti-M6PR showed that sortilin and M6PR were expressed in the endothelium of smaller and larger vessels. Uptake studies in cultured glomerular endothelial cells of α-Gal A labeled with fluorescence and (125)I showed by inhibition with RAP and M6P that sortilin and M6PR mediated uptake of α-Gal A. Biacore studies revealed that α-Gal A binds to human M6PR with very high affinity, but M6PR also binds to sortilin in a way that prevents α-Gal A binding to sortilin. Taken together, our data provide evidence that sortilin is a new α-Gal A receptor expressed in renal endothelial cells and that this receptor together with the M6PR is able to internalize circulating α-Gal A during enzyme replacement therapy in patients with Fabry disease.
FUNCTION OF ROOT BORDER CELLS IN PLANT HEALTH: Pioneersin the Rhizosphere (1998)

Plants dedicate a large amount of energy to the regulated production of living cells programmed to separate from roots into the external environment. This unusual process may be worth the cost because it enables the plant to dictate which species will share its ecological niche. For example, border cells can rapidly attract and stimulate growth in some microorganisms and repel and inhibit the growth of others. Such specificity may provide a way to control the dynamics of adjacent microbial populations in the soil to foster beneficial associations and inhibit pathogenic invasion. Plant genes controlling the delivery of border cells and the expression of their unique properties provide tools to genetically engineer plants with altered border cell quality and quantity. Such variants are being used to test the hypothesis that the function of border cells is to protect plant health by controlling the ecology of the root system.
Prehydrating High-strength Microspheres in Lightweight Cement Slurry Creates Value for Offshore Malaysian Operator (2013)

Abstract Casing cementation across depleted reservoirs can be challenging because conventionally weighted cement slurries can induce losses and damage the permeability of formations caused by the higher associated hydrostatic pressure they exert on the wellbore. Therefore, when such pressure-related concerns exist, lighter-weight cement slurries are often preferred. For an offshore Malaysia operator, a lightweight cement slurry was proposed to reduce hydrostatic pressure and prevent losses and formation damage. However, instead of a water-extended lightweight cement slurry, a high-performance, lightweight (HPLW) cement slurry consisting of high-strength, hollow microspheres was designed. Special bulk blending and transportation are normally required for these HPLW slurries because of the large bulk volume and low specific gravity (SG) of high-strength, hollow microspheres. In this case, because of limitations related to the rig’s storage capacity, the microspheres could not be stored conventionally and used as a dry additive to the slurry during mixing. Instead, an alternative solution involving prehydrating the high-strength microspheres with liquid additives and mix water in the mixing pits was implemented. This paper discusses the procedures used during cementing operations and details the value of using this mixing method.
Effect of Processing Parameters on Physical Properties of Cadmium Stannate Thin Films Prepared by a Dip‐Coating Technique (2001)

Thin films of dicadmium stannate spinel (Cd 2 SnO 4 ) were deposited on glass substrates using a dip‐coating technique. The films were transparent to visible light (90%) and electrically conductive. X‐ray diffractometry showed that annealed films consisted of a single cubic spinel phase only when they were prepared from a solution with the composition of Cd:Sn = 2.5 and fired at a temperature of 400°–500°C. The Cd:Sn ratio, the firing temperature, and the post‐deposition annealing sequence were crucial for the formation of a single phase, which is vital to obtain optimal optical and electrical properties. A resistivity as low as 3.3 × 10 −4 Ω·cm could be obtained after annealing.
Design on Intelligent Maintenance Device in large craft (2011)

With respect to maintenance in large aircraft, the issue is always focused on how to enhance the efficiency of Maintenance Instrument and improve the ability of human-interaction. Our designed Intelligent Maintenance Device (IMD) would meet those requirements. Based on ARM microprocessor and Embedded Linux Operating System, IMD we designed have the ability to detect and confirm the fault. By sampling and fitting voltages of component in large craft whose fault is need to be determined, maintainers can easily solve the problem while performing other work.
Focused ion beam tuning of in-plane vibrating micromechanicalresonators (1999)

Strategies for permanently tuning the resonant frequency of in-plane micromechanical resonators are compared. It is shown that tuning of the suspension stiffness may have advantages compared with tuning of the mass. Iterative frequency tuning of laterally resonant electrostatic comb-drive microactuators by focused ion beam machining is demonstrated.
Regulation of reactivated contraction in teleost retinal cone models by calcium and cyclic adenosine monophosphate. (1984)

We have been using lysed cell models of teleost retinal cones to examine the mechanism of contraction in nonmuscle cells. We have previously reported that dark-adapted retinas can be lysed with the detergent Brij-58 to obtain cone motile models that undergo Ca++- and adenosine triphosphate (ATP)-dependent reactivated contraction. In this report we further dissect the roles of ATP and Ca++ in activation of contraction and force production by (a) characterizing the Ca++ and nucleotide requirements in more detail, (b) by analyzing the effects of inosine triphosphate (ITP) and the ATP analog ATP gamma S and (c) by testing effects of cyclic adenosine monophosphate (cAMP) on reactivated cone contraction. Exposing lysed cone models to differing free Ca++ concentrations produced reactivated contraction at rates proportional to the free Ca++ concentration between 3.16 X 10(-8) and 10(-6) M. A role for calmodulin (CaM) in this Ca++ regulation was suggested by the inhibition of reactivated contraction by the calmodulin inhibitors trifluoperazine and calmidazolium ( R24571 ). The results of analysis of nucleotide requirements in lysed cone models were consistent with those of smooth muscle studies suggesting a role for myosin phosphorylation in Ca++ regulation of contraction. ATP gamma S and ITP are particularly interesting in that ATP gamma S, on the one hand, can be used by kinases to phosphorylate proteins (e.g., myosin light chains) but resists cleavage by phosphatases or adenosine triphosphatases (ATPases), e.g., myosin ATPase. ITP, on the other hand, can be used by myosin ATPase but does not support Ca++/calmodulin mediated phosphorylation of myosin light chains by myosin light chain kinase. Thus, these nucleotides provide an opportunity to distinguish between the kinase and myosin ATPase requirements for ATP. When individual nucleotides were tested with cone motile models, the nucleotide requirement was highly specific for ATP; not only ITP and ATP gamma S, but also guanosine triphosphate, cytosine triphosphate, adenylyl-imidodiphosphate (AMPPNP) failed to support reactivated contraction when substituted for ATP throughout the incubation. However, if lysed cones were initially incubated with ATP gamma S and then subsequently incubated with ITP, the cones contracted to an extent that was comparable to that observed with ATP. As observed in skinned smooth muscle, adding cAMP to contraction medium strongly inhibited contraction in lysed cone models.
Enzyme replacement therapy by fibroblast transplantation: long-term biochemical study in three cases of Hunter's syndrome. (1979)

We have assessed the effectiveness of transplanted histocompatible fibroblasts as a long-lived source of lysosomal enzymes for replacement therapy in three patients with Hunter's syndrome, over periods ranging from 2.5 to 3.75 yr. The level of Hunter corrective factor excreted by all three patients increased after transplantation, as did the activity of alpha-L-idurono-2-sulfate sulfatase in serum, when measured directly with a radioactive disulfated disaccharide substrate. Sulfatase activity was also raised in leukocyte homogenates from the two patients that we were able to assess. These increases in enzyme activity were accompanied by corresponding increases in catabolism of heparan and dermatan sulfates, as shown by (a) a decrease in sulfate:uronic ratios of urinary oligosaccharides, (b) an increase in iduronic acid monosaccharide, and (c) a normalization of Bio-Gel P-2 gel filtration profiles. Both the increase in enzyme activity and increased catabolism were maintained during the period of study and were not affected by either a gradual decrease or total withdrawal of immunosuppressive therapy.
Identification of a Domain of Axin That Binds to the Serine/Threonine Protein Phosphatase 2A and a Self-binding Domain (1999)

Axin is a negative regulator of embryonic axis formation in vertebrates, which acts through a Wnt signal transduction pathway involving the serine/threonine kinase GSK-3 and beta-catenin. Axin has been shown to have distinct binding sites for GSK-3 and beta-catenin and to promote the phosphorylation of beta-catenin and its consequent degradation. This provides an explanation for the ability of Axin to inhibit signaling through beta-catenin. In addition, a more N-terminal region of Axin binds to adenomatous polyposis coli (APC), a tumor suppressor protein that also regulates levels of beta-catenin. Here, we report the results of a yeast two-hybrid screen for proteins that interact with the C-terminal third of Axin, a region in which no binding sites for other proteins have previously been identified. We found that Axin can bind to the catalytic subunit of the serine/threonine protein phosphatase 2A through a domain between amino acids 632 and 836. This interaction was confirmed by in vitro binding studies as well as by co-immunoprecipitation of epitope-tagged proteins expressed in cultured cells. Our results suggest that protein phosphatase 2A might interact with the Axin.APC.GSK-3.beta-catenin complex, where it could modulate the effect of GSK-3 on beta-catenin or other proteins in the complex. We also identified a region of Axin that may allow it to form dimers or multimers. Through two-hybrid and co-immunoprecipitation studies, we demonstrated that the C-terminal 100 amino acids of Axin could bind to the same region as other Axin molecules.
Predictive microRNAs for lymph node metastasis in endoscopically resectable submucosal colorectal cancer (2016)

Accurate prediction of regional lymph node metastasis (LNM) in endoscopically resected T1-stage colorectal cancers (CRCs) can reduce unnecessary surgeries. To identify miRNA markers that can predict LNM in T1-stage CRCs, the study was conducted in two phases; (I) miRNA classifier construction by miRNA-array and quantitative reverse transcription PCR (qRT-PCR) using 36 T1-stage CRC samples; (II) miRNA classifier validation in an independent set of 20 T1-stage CRC samples. The expression of potential downstream target genes of miRNAs was assessed by immunohistochemistry. In the discovery analysis by miRNA microarray, expression of 66 miRNAs were significantly different between LNM-positive and negative CRCs. After qRT-PCR validation, 11 miRNAs were consistently significant in the combined classifier construction set. Among them, miR-342-3p was the most significant one (P=4.3×10-4). Through logistic regression analysis, we developed a three-miRNA classifier (miR-342-3p, miR-361-3p, and miR-3621) for predicting LNM in T1-stage CRCs, yielding the area under the curve of 0.947 (94% sensitivity, 85% specificity and 89% accuracy). The discriminative ability of this system was consistently reliable in the independent validation set (83% sensitivity, 64% specificity and 70% of accuracy). Of the potential downstream targets of the three-miRNAs, expressions of E2F1, RAP2B, and AKT1 were significantly associated with LNM. In conclusion, this classifier can predict LNM more accurately than conventional pathologic criteria and our study results may be helpful to avoid unnecessary bowel surgery after endoscopic resection in early CRC.
Kinetic studies of Escherichia coli elongation factor Tu-guanosine 5'-triphosphate-aminoacyl-tRNA complexes (1985)

A new method for measuring the dissociation rate of the Escherichia coli elongation factor Tu-GTP--aminoacyl-tRNA complex has been developed and applied to the determination of the dissociation rates of ternary complexes formed between E. coli EF-Tu-GTP and a set of E. coli aminoacyl-tRNAs. The set of aminoacyl-tRNAs includes at least one tRNA coding for each of the 20 amino acids as well as purified isoacceptor tRNA species for arginine, glycine, leucine, lysine, and tyrosine. The results reveal that the dissociation rates vary for each ternary complex. Tu-GTP-Gln-tRNA dissociates the slowest and Tu-GTP-Val-tRNA the fastest of all noninitiator ternary complexes at 4 degrees C, pH 7.4. The equilibrium dissociation constant for Tu-GTP-Thr-tRNA has been determined to be 1.3 (0.4) X 10(-9) M under identical reaction conditions, and the absolute value of the equilibrium dissociation constant has been calculated for 28 ternary complexes from the relative equilibrium dissociation constant ratios previously measured [Louie, A., Ribeiro, N. S., Reid, B. R., & Jurnak, F. (1984) J. Biol. Chem. 259, 5010-5016]. The association rate of each ternary complex has been estimated from the ratio of the dissociation rate relative to the equilibrium dissociation constant. Tu-GTP-His-tRNA associates the fastest and Tu-GTP-Leu-tRNA1Leu the slowest. By inclusion of Tu-GTP-Met-tRNAfMet in the studies, evidence has been obtained that suggests that the initiator ternary complex does not function in the elongation cycle because the dissociation rate of the complex is very fast.
Transient crowd discovery on the real-time social web (2011)

In this paper, we study the problem of automatically discovering and tracking transient crowds in highly-dynamic social messaging systems like Twitter and Facebook. Unlike the more static and long-lived group-based membership offered on many social networks (e.g., fan of the LA Lakers), a transient crowd is a short-lived ad-hoc collection of users, representing a "hotspot" on the real-time web. Successful detection of these hotspots can positively impact related research directions in online event detection, content personalization, social information discovery, etc. Concretely, we propose to model crowd formation and dispersion through a message-based communication clustering approach over time-evolving graphs that captures the natural conversational nature of social messaging systems. Two of the salient features of the proposed approach are (i) an efficient locality- based clustering approach for identifying crowds of users in near real-time compared to more heavyweight static clustering algorithms; and (ii) a novel crowd tracking and evolution approach for linking crowds across time periods. We find that the locality-based clustering approach results in empirically high-quality clusters relative to static graph clus- tering techniques at a fraction of the computational cost. Based on a three month snapshot of Twitter consisting of 711,612 users and 61.3 million messages, we show how the proposed approach can successfully identify and track interesting crowds based on the Twitter communication structure and uncover crowd-based topics of interest.
Silver carboxylate nanostructure nucleation and growth on AgBr crystals (2005)

The initial stage of the reaction between sodium stearate (NaSt) and AgNO(3) produces silver stearate (AgSt) micelles, [(C(18)H(35)O(2))(x)(Na(x-y))(Ag(y))(H(2)O)(z)], and aggregations of these AgSt micelles in the form of cubic pre-AgSt crystals. When cubic grains of 50 nm AgBr are added to the NaSt dispersion prior to the AgNO(3), the reaction proceeds to form the silver stearate micelles, but not the aggregation of those micelles. Instead, the {111} silver ion planes of the cubic AgBr crystal corners provide nucleation sites for silver stearate micelle deposition and crystal growth. After nucleation, the AgSt micelles evolve into nanostructured bud-like formations via an epitaxial interface on one or several corners of each AgBr cubic crystal. Over time, additional AgSt micelle deposition enables the buds to grow longer into strand-like structures, which then connect to form the beginnings of the ultimate silver stearate crystal plates.
Relationship between chloride concentration and electrical conductivity in groundwater and its estimation from vertical electrical soundings (VESs) in Guasave, Sinaloa, Mexico (2012)

Relationship between chloride concentration and electrical conductivity in groundwater and its estimation from vertical
Ferromagnetic properties of Zn1−xMnxO epitaxial thin films (2002)

We report on ferromagnetic characteristics of Zn1−xMnxO (x=0.1 and 0.3) thin films grown on Al2O3(00⋅1) substrates using laser molecular-beam epitaxy. By increasing the Mn content, the films exhibited increases in both the c-axis lattice constant and fundamental band gap energy. The Curie temperature obtained from temperature-dependent magnetization curves was 45 K for the film with x=0.3, depending on the Mn composition in the films. The remanent magnetization and coercive field of Zn0.9Mn0.1O at 5 K were 0.9 emu/g and 300 Oe, respectively. For Zn0.7Mn0.3O, the remanent magnetization at 5 K increased to 3.4 emu/g.
A planar varactor diode for harmonic generation at gigahertz frequencies (2002)

The authors present the development of a planar varactor diode to eliminate whisker contact and thereby create a local oscillator power source for high-frequency, space-based heterodyne receivers. Specifically, the design and fabrication of a planar GaAs varactor diode for use as a 75-GHz-to-225-GHz tripler are described. It is concluded that the excellent electrical characteristics and ruggedness of the planar varactor diodes described here make them ideally suited for use in space-based systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Comparison of Yields and Fruit Quality between 'Matsumotowase-Fuyu' Japanese Persimmon Trees Trained to a Horizontal Trellis and Open Center Free-Standing Ones (2004)

To reduce labor costs in the cultivation of Japanese persimmon which is prone to be too vigorous, a horizontal trellis training system was attempted as a practical method for lowering the tree height. The frameworks of an open center, 3.6 m high 'Matsumotowase-Fuyu' Japanese persimmon trees were altered to a horizontal frame at 1.8 m height on February 1992 by cutting back secondary scaffold branches at 1.5. to 1.6 m above ground level. The tree growth, yields and fruit quality of the altered trees were compared with the open-center free-standing trees control. One year after altering the framework, the canopy area on horizontally trellised trees expanded vigorously, compared with the control group. After pruning, the trees with altered the framework were left with twenty percent more lateral branches than the open center free-standing trees. Two years after altering, the number of shoots per canopy area was greater in the treated trees than in the control, but the number of shoots per lateral branches and mean shoot length did not significantly differ from the control group. Annual fruit yield was 300 kg/a higher in the horizontal trellis system than that of the unaltered free standing system group, and the number of fruits per canopy area exceeded 10 fruits/m2. Over 4-year period, the horizontally trellised trees produced more flowers per lateral branch than did the open center free-standing ones. Physiological fruit drop rates were lower in horizontally trellised trees than in those of the control. Fruit on the former was significantly heavier than that on the letter. The percentage of fruit weighing more than 260 g accounted for 61.2% of the total yield in horizontally trellised trees. During the period of 80 days after blossoming to harvest, fruit diameter increased significantly faster on horizontally trellised trees than that on the free standing trees. Similarly, the commencement of fruit skin coloring and harvest time was advanced in the treated trees compared with those of the control. In conclusion, by lowering tree height the horizontal trellis training system achieves the following: decreased harvest and pruning costs, increased yield and fruit weight, improved fruit quality, and advanced maturation. These advantages indicate that this system has a strong potential as a training system that will be used extensively in the near future.