gebhart/patrepeval huggingface dataset:
Reversal of resistance to vincristine in P388 leukemia by various polycyclic clinical drugs, with a special emphasis on quinacrine. (1988)

We investigated several lipophilic drugs with a polycyclic structure for their effect on the net uptake of vincristine in vincristine-resistant P388 leukemia cells. Fourteen of 23 agents promoted vincristine uptake in the resistant cells. The net increase in vincristine uptake was caused by prevention of its outward transport rather than by stimulation of inward transport. Some of these drugs, e.g., quinacrine, dilazep, syrosingopine, simetride, etc., remarkably potentiated the cytotoxicity of vincristine against the resistant cells in vitro. Quinacrine, an antimalarial drug which had the greatest effect on vincristine uptake and relatively low host toxicity, exhibited potent therapeutic synergism in combination with vincristine in resistant leukemia-bearing mice.
Evaluation of 802.11a for streaming data in ad-hoc networks (2004)

Advances in communication and processing have made ad-hoc networks of wireless devices a reality. One application is home entertainment systems where multiple Home-to-Home (H20) devices collaborate as peers to stream audio and video clips to a household. In this study, we investigate the feasibility of IEEE 802.11a protocol in combination with both TCP and UDP to realize a H20 device. Challenges include lossy connections, unfair allocation of bandwidth between multiple simultaneous transmissions, and the exposed node limitation [22], [19], [13], [4]. Our primary contribution is an empirical study of 802.11a to quantify these factors and their significance. Our multi-dimensional experimental design consists of the following axes: distance between participating devices, number of intermediate H20 devices used to route a stream from a producing H20 device to a consuming H2O device, and simultaneous number of active streams in the same radio range. Both operating system and application level routing were considered_ Obtained results demonstrate the following lessons. First, with a multi-hop UDP transmission, in the absence of congestion control, transient bottlenecks result in a high loss rate. Hence, a transport protocol with congestion control is essential for streaming of continuous media within a H2O cloud. Second, 802.11a does not drop TCP connections in the presence of many competing transmissions (802.11b drops connections [22]). Third, we observed fairness when transmitting several hundred Megabytes (MB) of data, among multiple competing 1- hop TCP and UDP flows. Fourth, while there is unfair allocation of bandwidth with an exposed node, the observed bandwidths are sufficient to stream a high-quality video clip (with a 4 Mbps display bandwidth requirement). These results indicate streaming of data is feasible with an ad-hoc network of wireless devices employing the 80211a protocol.
An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence. (1994)

Regions extremely variable in size and sequence occur at conserved locations in eukaryotic rRNAs. The functional importance of one such region was determined by gene reconstruction and replacement in Tetrahymena thermophila. Deletion of the D8 region of the large-subunit rRNA inactivates T. thermophila rRNA genes (rDNA): transformants containing only this type of rDNA are unable to grow. Replacement with an unrelated sequence of similar size or a variable region from a different position in the rRNA also inactivated the rDNA. Mutant rRNAs resulting from such constructs were present only in precursor forms, suggesting that these rRNAs are deficient in either processing or stabilization of the mature form. Replacement with D8 regions from three other organisms restored function, even though the sequences are very different. Thus, these D8 regions share an essential functional feature that is not reflected in their primary sequences. Similar tertiary structures may be the quality these sequences share that allows them to function interchangeably.
A new approach to the determination of the shear strength of clay by the fall-cone test (1957)

(1) Region disturbed by the cone; (2) Study of the cone motion; (3) Relation between shear strength and cone penetration; (4) Precautions to be observed in the fall-cone test; (5) Comparison between the fall-cone test and other shear strengthtests.
ARQ with Doped Fountain Decoding (2008)

We analyze the benefits of an automatic repeat- request (ARQ) scheme employed in doped belief-propagation decoding for increasing the throughput of fountain encoded data transmissions. The proposed doping mechanism selects doping symbols randomly from the set of input symbols contributing to degree-two output symbols. Here an output symbol is the encoded symbol whose code-graph links to input symbols decoded thus far have been severed. This doping approach always ensures releasing of at least one output symbol, thus increasing the number of degree-one output symbols (the ripple). Using a random walk analysis, we study the belief propagation decoding with degree-two random doping for a fountain code with symbols drawn from an Ideal Soliton distribution. We show that the decoding process is a renewal process whereas the process starts all over afresh after each doping. The approximate interdoping process analysis revolves around a random walk model for the ripple size. We model the sequence of the ripple size increments (due to doping and/or decoding) as an iid sequence of shifted and truncated Poisson random variables. This model furnishes a prediction on the number of required doping symbols and, furthermore, the ARQ throughput cost analysis. We also find that the Ideal Soliton significantly outperforms the Robust Soliton distribution in our ARQ-doping scheme.
Concentration-Thermal Swing Adsorption Process for Separation of Bulk Liquid Mixtures (1993)

Abstract A novel concentration-thermal swing adsorption process is described for separation of bulk binary liquid mixtures. The process is designed to produce essentially two pure products with high recoveries of both components. It is particularly suited for separation of azeotropic or close-boiling liquid mixtures which are difficult to separate by distillation. An example of the performance of the new process for separation of an azeotropic water-methyl acetate mixture is given. Experimental binary surface excess equilibrium isotherms, adsorptive mass transfer coefficients, and column dynamics for adsorption of water-methyl acetate mixtures on NaX zeolite are reported.
Transaction processing primitives and CSP (1987)

Several primitives for transaction processing systems are developed using the notations of Communicating Sequential Processes. The approach taken is to capture each requirement separately, in the simplest possible context: The specification is then the conjunction of all these requirements. As each is developed as a predicate over traces of the observable events in the system, it is also implemented as a simple communicating process; the implementation of the entire system is then merely the parallel composition of these processes. The laws of CSP are then used to transform the system to achieve the required degree of concurrency, to make it suitable for execution in a multiple-tasking system, for example. Finally, there is a discussion of how state-based systems may be developed using this approach together with some appropriate notation for specifying and refining data structures and operations upon them and of how the system may be implemented. This work is intended as a case study in the use of CSP.
Some Physical Characteristics of a Proteinase from Aeromonas proteolytica (1970)

Abstract A proteinase has been isolated from culture filtrates of Aeromonas proteolytica essentially free of the aminopeptidase activity that is present in spent media from this organism. Sedimentation and moving boundary electrophoretic experiments indicated a high degree of homogeneity for the enzyme, which was isoelectric at approximately pH 3.5. The proteinase had a sedimentation coefficient of 3.50 S, a diffusion coefficient (d020,w) of 8.52 x 10-7 cm2 sec-1, and a partial specific volume, calculated from the amino acid composition, of 0.709 cm3 g-1. From both sedimentation velocity and sedimentation equilibrium, the molecular weight was 34,800; calculations of minimum molecular weight on the basis of zinc content yielded a value near 33,800 which indicated the presence of 1 g atom of zinc per mole of enzyme. Amino acid analysis revealed approximately 316 residues.
Simplified Real-Time Multiplex Detection of Loop-Mediated Isothermal Amplification Using Novel Mediator Displacement Probes with Universal Reporters (2018)

A variety of real-time detection techniques for loop-mediated isothermal amplification (LAMP) based on the change in fluorescence intensity during DNA amplification enable simultaneous detection of multiple targets. However, these techniques depend on fluorogenic probes containing target-specific sequences. That complicates the adaption to different targets leading to time-consuming assay optimization. Here, we present the first universal real-time detection technique for multiplex LAMP. The novel approach allows simple assay design and is easy to implement for various targets. The innovation features a mediator displacement probe and a universal reporter. During amplification of target DNA the mediator is displaced from the mediator displacement probe. Then it hybridizes to the reporter generating a fluorescence signal. The novel mediator displacement (MD) detection was validated against state-of-the-art molecular beacon (MB) detection by means of a HIV-1 RT-LAMP: MD surpassed MB detection by accelerated probe design (MD: 10 min, MB: 3-4 h), shorter times to positive (MD 4.1 ± 0.1 min shorter than MB, n = 36), improved signal-to-noise fluorescence ratio (MD: 5.9 ± 0.4, MB: 2.7 ± 0.4; n = 15), and showed equally good or better analytical performance parameters. The usability of one universal mediator-reporter set in different multiplex assays was successfully demonstrated for a biplex RT-LAMP of HIV-1 and HTLV-1 and a biplex LAMP of Haemophilus ducreyi and Treponema pallidum, both showing good correlation between target concentration and time to positive. Due to its simple implementation it is suggested to extend the use of the universal mediator-reporter sets to the detection of various other diagnostic panels.
Content-based multimedia data access in Internet video communication (2002)

This paper presents a platform for the development and evaluation of MPEG-4 functionalities. The implemented functionalities are content-based multimedia data access, content-based manipulation and bitstream editing, hybrid natural and synthetic data combination, content-based scalability. The system was implemented with Java.
Effects of different pretreatments on the surface structure of silicon and the adhesion of metal films (1992)

Interface structures and adhesion to silicon after different surface pretreatments have been investigated for Ni(500–1000 nm)/Ti(250 nm) films prepared in a dc planar magnetron sputtering apparatus. In order to obtain high adhesion, a chemical pretreatment (with buffered HF) has been found to be favorable in comparison with a conventional Ar ion bombardment pretreatment (with cathodic voltage: 400 V). High resolution transmission electron microscopy (HRTEM) showed that there were two layers between Ti and Si in the case of Ar ion bombardment pretreatment. Energy dispersive x-ray spectroscopy (EDS) and electron diffraction (ED) showed that they were amorphous Ti–Si alloy and amorphous Si containing Ar. Moreover, a peeling pattern, after the test with adhesive tape, could be found at the boundary between the amorphous Ti–Si alloy layer and the amorphous Si layer containing Ar by using x-ray photoelectron spectroscopy (XPS). In the case of chemical pretreatment, only an amorphous Ti–Si alloy layer was observed between Ti and Si, and peeling was not observed. The differences in interface structure and adhesion are considered to be influenced by the Ar implanted during the Ar ion bombardment pretreatment.
Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock (2011)

The function of phagocytic and antigen presenting cells is of crucial importance to sustain immune competence against infectious agents as well as malignancies. We here describe a reproducible procedure for the quantification of phagocytosis by leukocytes in whole blood. For this, a pH-sensitive green-fluorescent protein- (GFP) like dye (Eos-FP) is transfected into infectious microroganisms. After UV-irradiation, the transfected bacteria emit green (≈5160 nm) and red (≈581 nm) fluorescent light at 490 nm excitation. Since the red fluorescent light is sensitive to acidic pH, the phagocytosed bacteria stop emitting red fluorescent light as soon as the phagosomes fuse with lysosomes. The green fluorescence is maintained in the phagolysosome until pathogen degradation is completed. Fluorescence emission can be followed by flow cytometry with filter settings documenting fluorescence 1 (FL 1, FITC) and fluorescence 2 (FL 2, phycoerythrin, PE). Eos-FP transfected bacteria can also be traced within phagocytes using microscopical techniques. A standardized assay has been developed which is suitable for clinical studies by providing clinicians with syringes pre-filled with fixed and appropriately UV-irradiated Eos-FP E. coli (TruCulture™). After adding blood or body fluids to these containers and starting the incubation at 37°C, phagocytosis by granulocytes proceeds over time. Cultures can be terminated at a given time by lysing red blood cells followed by flow cytometry. A pilot study demonstrated that Eos-FP E. coli phagocytosis and digestion was up-regulated in the majority of patients with either severe sepsis or septic shock as compared to healthy donors (p < 0.0001 after o/n incubation). Following treatment with recombinant human granulocyte colony-stimulating factor (rhG-CSF) in selected patients with sepsis, phagolysosome fusion appeared to be accelerated.
Targeted Inactivation of the Mouse α2-Macroglobulin Gene (1995)

The mouse alpha 2-macroglobulin gene was inactivated in embryonic stem cells by homologous recombination. Liver alpha 2-macroglobulin mRNA and plasma protein was absent in homozygotes and reduced to 50% in heterozygotes. alpha 2-Macroglobulin-deficient mice were viable and produced normally sized litters with normal sex ratio over 3 generations. Characterization of adult homozygotes included diets with different fat content, treatments with endotoxin, bleomycin, carbon tetrachloride, and ethionine to test for immune system, lung, liver, and pancreas toxicity, respectively. Knock-out mice were more resistant to endotoxin but more sensitive to a choline-free diet supplemented with ethionine. Regulation of murinoglobulin mRNA expression during pregnancy was analyzed as a possible back-up mechanism for the deficiency in alpha 2-macroglobulin. In addition, expression of mRNA was studied, coding for alpha 2-macroglobulin receptor/lipoprotein receptor-related protein, low density lipoprotein receptor, and very low density lipoprotein receptor and for some common ligands, i.e. apolipoprotein E, lipoprotein lipase, and the 44-kDa heparin binding protein. Their differential regulation in the knock-out mice relative to C57B1 mice was evident and is discussed. The impressive 15-fold increase in maternal liver murinoglobulin mRNA at partum in the knock-out mice indicated increased consumption, compared to only 4-fold in normal mice. Thus, murinoglobulin appears as the major proteinase inhibitor around partum, obviously solicited to a much greater extend in alpha 2-macroglobulin-deficient mice.
EFFICIENT CW LASER OSCILLATION AT 4416 Å IN Cd (II) (1968)

A gain of 20%/m and a power output of 50 mW have been measured on the 5s2 2D5/2 → 5p2P03/2 4416-Å laser transition in Cd (II) using a single isotope of Cd114. The system was operated in a tubular furnace at temperatures in the range of 200°C with He as a buffer gas. The laser output with respect to He pressure, discharge current, and furnace temperature are discussed along with a proposed excitation mechanism. The system appears to have the prospect of being one of the most efficient, high power lasers operating in the visible region of the spectrum.
Electronic and structural properties of the pentanary alloy GaxIn1−xPySbzAs1−y−z (1999)

The universal tight binding (UTB) model based on a modified pseudocell (MPC) is used to obtain the electronic band structure, band gaps, and the density of states (DOS) for the pentanary alloy GaxIn1−xPySbzAs1−y−z of dimension N=3 (i.e., x, y, and z). The composition dependent local and alloy bond lengths of the alloy are formulated within the MPC, and the lattice matching conditions for several substrates are found by optimizing the bond length of the substrate to the alloy bond length. The principal band gaps [E(Γ),E(L),E(X)] of the alloy are calculated lattice matched to GaAs, InP, InAs, and GaSb. The main contribution of each component upon the DOS is investigated through observation of variations with composition. In addition, the band gap calculations are performed for the ternary (N=1; GaxIn1−xP, GaPyAs1−y, and GaxIn1−xAs) and quaternary (N=2; GaxIn1−xPyAs1−y) alloys which are limiting composition cases for the pentanary alloy GaxIn1−xPySbzAs1−y−z. The latter reduced results are in good agreement with available experimental data, thereby validating the UTB model. The UTB model may also be extended to treat higher dimensional material systems (N⩾4).
Restricted T-cell receptor V beta gene usage by myelin basic protein-specific T-cell clones in multiple sclerosis: predominant genes vary in individuals. (1991)

Recent studies in experimental autoimmune encephalomyelitis as a model for multiple sclerosis (MS) have demonstrated limited heterogeneity in T-cell antigen receptors (TCR) specific for myelin basic protein (MBP). To investigate restricted beta-chain variable-region (V beta) gene usage in humans, we analyzed TCR gene rearrangements in two lines and 34 MBP-specific T-cell clones that were isolated from five MS patients and two healthy subjects. The T cells were characterized for their specificity to MBP epitopes and HLA-restricting molecules. We demonstrate here that MBP-specific T-cell clones from these different MS patients and healthy individuals, in contrast to T cells from rodents, display a more diverse V beta gene usage as evidenced by their TCR V beta gene rearrangements. However, the different MBP-specific T-cell clones isolated from each individual MS patient showed a common V beta gene usage, suggesting individual-specific TCR restriction. Out of 16 MBP-specific clones derived from a single MS patient, 12 clones (75%) utilized the V beta 15 gene for their TCR gene rearrangement. MBP-specific clones isolated from four other MS patients also showed a consistent tendency for a predominant, but different, TCR V beta gene rearrangement. These results suggest a TCR heterogeneity among MBP-specific T-cell clones from different individuals but a limited TCR V beta gene usage among MBP-specific T-cell clones of the same individual. The predominant V beta gene used by the MBP-specific T-cell clones studied here was not found to correlate with the epitope specificity of T cells or with their restricting HLA molecule. These findings may support the possibility of intervention with monoclonal antibodies to specific V beta gene products as an approach to immune therapy of MS but also imply the necessity for an individual-specific immunotherapeutic approach.
A Faraday Effect Optical Isolator (1964)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text L. J. Aplet and J. W. Carson, "A Faraday Effect Optical Isolator," Appl. Opt. 3, 544-545 (1964) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Long-read sequence analysis of the MECP2 gene in Rett syndrome patients: correlation of disease severity with mutation type and location (2000)

Mutations in the methyl-CpG-binding protein gene MECP2 at Xq28 cause Rett syndrome (RTT), an X-linked dominant neurodevelopmental disorder characterized by a period of stagnation followed by regression in the development of young girls. Mutations were sought in MECP2 in 48 females with classical sporadic RTT, seven families with possible familial RTT and five sporadic females with features suggestive, but not diagnostic of RTT. Long distance PCR coupled with long-read direct sequencing was employed to sequence the entire MECP2 gene coding region in all cases. Mutations were identified in 44/55 (80%) unrelated classical sporadic and familial RTT patients, but only 1/5 (20%) sporadic cases with suggestive but non-diagnostic features of RTT. Twenty-one different mutations were identified (12 missense, four nonsense and five frame-shift mutations); 14 of these were novel. All missense mutations were located either in the methyl-CpG-binding domain or in the transcription repression domain. Nine recurrent mutations were characterized in a total of 33 unrelated cases (73% of all cases with MECP2 mutations). Significantly milder disease was noted in patients carrying missense mutations as compared with those with truncating mutations ( P = 0. 0023), and milder disease was associated with late as compared with early truncating mutations ( P = 0.0190).
Experiments with Reinforcement Learning in Problems with Continuous State and Action Spaces (1997)

A key element in the solution of reinforcement learning problems is the value function. The purpose of this function is to measure the long-term utility or value of any given state. The function is important because an agent can use this measure to decide what to do next. A common problem in reinforcement learning when applied to systems having continuous states and action spaces is that the value function must operate with a domain consisting of real-valued variables, which means that it should be able to represent the value of infinitely many state and action pairs. For this reason, function approximators are used to represent the value function when a close-form solution of the optimal policy is not available. In this article, we extend a previously proposed reinforcement learning algorithm so that it can be used with function approximators that generalize the value of individual experiences across both state and action spaces. In particular, we discuss the benefits of using sparse coarse-coded function approximators to represent value functions and describe in detail three implementations: cerebellar model articulation controllers, instance-based, and case-based. Additionally, we discuss how function approximators having different degrees of resolution in different regions of the state and action spaces may influence the performance and learning efficiency of the agent. We propose a simple and modular technique that can be used to implement function approximators with nonuniform degrees of resolution so that the value function can be represented with higher accuracy in important regions of the state and action spaces. We performed extensive experiments in the double-integrator and pendulum swing-up systems to demonstrate the proposed ideas. '