gebhart/patrepeval huggingface dataset:
Manhattan Scene Understanding via XSlit Imaging (2013)

A Manhattan World (MW) [3] is composed of planar surfaces and parallel lines aligned with three mutually orthogonal principal axes. Traditional MW understanding algorithms rely on geometry priors such as the vanishing points and reference (ground) planes for grouping coplanar structures. In this paper, we present a novel single-image MW reconstruction algorithm from the perspective of non-pinhole cameras. We show that by acquiring the MW using an XSlit camera, we can instantly resolve co planarity ambiguities. Specifically, we prove that parallel 3D lines map to 2D curves in an XSlit image and they converge at an XSlit Vanishing Point (XVP). In addition, if the lines are coplanar, their curved images will intersect at a second common pixel that we call Coplanar Common Point (CCP). CCP is a unique image feature in XSlit cameras that does not exist in pinholes. We present a comprehensive theory to analyze XVPs and CCPs in a MW scene and study how to recover 3D geometry in a complex MW scene from XVPs and CCPs. Finally, we build a prototype XSlit camera by using two layers of cylindrical lenses. Experimental results on both synthetic and real data show that our new XSlit-camera-based solution provides an effective and reliable solution for MW understanding.
TREATMENT OF STEROID RESISTANT REJECTION WITH A NOVEL ANTI B-CELL PREPARATION, RITUXIMAB. (2000)

Becker, Yolanda T.1; Becker, Bryan N.2; Knechtle, Stuart J.1; Odorico, Jon S.1; D'Alessandro, Anthony M.1; Pirsch, John D.1; Sollinger, Hans W.1 Author Information
<i>Lactococcus lactis</i> Lytic Bacteriophages of the P335 Group Are Inhibited by Overexpression of a Truncated CI Repressor (2002)

Phages of the P335 group have recently emerged as important taxa among lactococcal phages that disrupt dairy fermentations. DNA sequencing has revealed extensive homologies between the lytic and temperate phages of this group. The P335 lytic phage phi31 encodes a genetic switch region of cI and cro homologs but lacks the phage attachment site and integrase necessary to establish lysogeny. When the putative cI repressor gene of phage phi31 was subcloned into the medium-copy-number vector pAK80, no superinfection immunity was conferred to the host, Lactococcus lactis subsp. lactis NCK203, indicating that the wild-type CI repressor was dysfunctional. Attempts to clone the full-length cI gene in Lactococcus in the high-copy-number shuttle vector pTRKH2 were unsuccessful. The single clone that was recovered harbored an ochre mutation in the cI gene after the first 128 amino acids of the predicted 180-amino-acid protein. In the presence of the truncated CI construct, pTRKH2::CI-per1, phage phi31 was inhibited to an efficiency of plaquing (EOP) of 10(-6) in NCK203. A pTRKH2 subclone which lacked the DNA downstream of the ochre mutation, pTRKH2::CI-per2, confirmed the phenotype and further reduced the phi31 EOP to <10(-7). Phage phi31 mutants, partially resistant to CI-per, were isolated and showed changes in two of three putative operator sites for CI and Cro binding. Both the wild-type and truncated CI proteins bound the two wild-type operators in gel mobility shift experiments, but the mutated operators were not bound by the truncated CI. Twelve of 16 lytic P335 group phages failed to form plaques on L. lactis harboring pTRKH2::CI-per2, while 4 phages formed plaques at normal efficiencies. Comparisons of amino acid and DNA level homologies with other lactococcal temperate phage repressors suggest that evolutionary events may have led to inactivation of the phi31 CI repressor. This study demonstrated that a number of different P335 phages, lytic for L. lactis NCK203, have a common operator region which can be targeted by a truncated derivative of a dysfunctional CI repressor.
Further evidence for recessive inheritance of von willebrand disease with abnormal binding of von willebrand factor to factor VIII (1992)

A new family with a bleeding diathesis and FVIII deficiency secondary to abnormal binding of von Willebrand factor (vWF) to factor VIII (FVIII) is described. Two propositi of this family, an 18-year-old male and a 33-year-old female, both with a history of epistaxis, bruising, bleeding from the gums, epistaxis, hemarthrosis, and hematoma, were analyzed. Also additional members of the same family with no bleeding history were also studied. The propositi showed normal vWF activities, low FVIII activity; one of them had been diagnosed as having hemophilia A and the other was a hemophilia A carrier. Both showed a very poor response to treatment with FVIII concentrates and desmopressin (DDAVP) but a good clinical response to cryoprecipitate. APTT was prolonged and no inhibitory activity was noticeable in their plasmas. Thirty-five units per kilogram body weight of Hemofil M was infused to both propositi and FVIII reached basal level within 60 minutes of the infusion. No FVIII response at all was observed in the female after intravenous DDAVP administration. However, the male who received the infusion of 35 U/kg body weight of Humate-P achieved a normal FVIII level that was maintained for 12 hours. Multimeric analysis of vWF was normal in all the members studied. Von Willebrand factor domain for FVIII binding was assayed in the two propositi and in six other members of the same family by using a non-isotopic and sensitive method, a modification of the one previously described, using the Hemofil M concentrate as exogenous FVIII. The data obtained showed that both propositi had similar binding to that observed by using plasma of a patient with severe von Willebrand disease. Furthermore, five siblings had a decreased binding of vWF to FVIII, when compared with plasma from normal individuals or patients with hemophilia A. We also observed that, for screening purpose, the ratio of bound FVIII/immobilized vWF (at saturation of the anti-vWF and offering of 1 U/ml of exogenous FVIII) distinguished two levels of abnormality (normal range 0.70-1.15, propositi 0.004-0.007, and remaining members affected 0.25-0.42). The most probable explanation is that the propositi are homozygous or double heterozygous, the other five siblings affected being heterozygous for a recessive vWF defect. This more accessible assay presented here may be of help in routine analysis for diagnosing this type of von Willebrand disease, which has important implications for therapy and genetic counseling.
Automatic design of modulators from specifications to silicon (1994)

We present a tool developed as part of the ESPRIT-AD2000 Project which, starting from high-level specifications of SC Sigma Delta modulators, calculates optimum specifications for their building blocks and then, optimum sizes for the block schematics. At both design levels, optimization is performed using statistical techniques to enable global design, and innovative heuristics for increased computer efficiency as compared with conventional statistical optimization approaches. We include measurements taken from a 17 bit @ 40 kHz prototype of an SC fully-differential fourth-order Sigma Delta modulator which was designed using the proposed tool for a 1.2 mu m CMOS double-metal double-poly technology.
Quantification of mRNA by non-radioactive RT-PCR and CCD imaging system (1992)

Journal Article Quantification of mRNA by non-radioactive RT-PCR and CCD imaging system Get access Hiroki Nakayama, Hiroki Nakayama Department of Clinical and Molecular Biology, Faculty of Medicine, Kyoto UniversityKyoto 606, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Hiromichi Yokoi, Hiromichi Yokoi Department of Clinical and Molecular Biology, Faculty of Medicine, Kyoto UniversityKyoto 606, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Jun Fujita Jun Fujita Department of Clinical and Molecular Biology, Faculty of Medicine, Kyoto UniversityKyoto 606, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 20, Issue 18, 25 September 1992, Page 4939, https://doi.org/10.1093/nar/20.18.4939 Published: 25 September 1992 Article history Received: 05 June 1992 Published: 25 September 1992
Visual compatibility of tolazoline hydrochloride with various medications during simulated Y-site injection (1990)

Journal Article Visual compatibility of tolazoline hydrochloride with various medications during simulated Y-site injection Get access Eric D. Marquardt, Pharm.D. Eric D. Marquardt, Pharm.D. Clinical Pharmacist Department of Pharmacy Services, University of California Davis Medical Center, Sacramento, and Assistant Clinical Professor of Pharmacy, School of Pharmacy, University of California San Franciso Address reprint requests to Dr. Marquardt at the Department of Pharmacy, University of California Davis Medical Center, 2315 Stockton Boulevard, Sacramento, CA 95817. Search for other works by this author on: Oxford Academic Google Scholar American Journal of Hospital Pharmacy, Volume 47, Issue 8, 1 August 1990, Pages 1802–1803, https://doi.org/10.1093/ajhp/47.8.1802 Published: 01 August 1990
RHAMM Promotes Interphase Microtubule Instability and Mitotic Spindle Integrity through MEK1/ERK1/2 Activity (2010)

An oncogenic form of RHAMM (receptor for hyaluronan-mediated motility, mouse, amino acids 163-794 termed RHAMM(Delta163)) is a cell surface hyaluronan receptor and mitotic spindle protein that is highly expressed in aggressive human cancers. Its regulation of mitotic spindle integrity is thought to contribute to tumor progression, but the molecular mechanisms underlying this function have not previously been defined. Here, we report that intracellular RHAMM(Delta163) modifies the stability of interphase and mitotic spindle microtubules through ERK1/2 activity. RHAMM(-/-) mouse embryonic fibroblasts exhibit strongly acetylated interphase microtubules, multi-pole mitotic spindles, aberrant chromosome segregation, and inappropriate cytokinesis during mitosis. These defects are rescued by either expression of RHAMM or mutant active MEK1. Mutational analyses show that RHAMM(Delta163) binds to alpha- and beta-tubulin protein via a carboxyl-terminal leucine zipper, but in vitro analyses indicate this interaction does not directly contribute to tubulin polymerization/stability. Co-immunoprecipitation and pulldown assays reveal complexes of RHAMM(Delta163), ERK1/2-MEK1, and alpha- and beta-tubulin and demonstrate direct binding of RHAMM(Delta163) to ERK1 via a D-site motif. In vitro kinase analyses, expression of mutant RHAMM(Delta163) defective in ERK1 binding in mouse embryonic fibroblasts, and blocking MEK1 activity collectively confirm that the effect of RHAMM(Delta163) on interphase and mitotic spindle microtubules is mediated by ERK1/2 activity. Our results suggest a model wherein intracellular RHAMM(Delta163) functions as an adaptor protein to control microtubule polymerization during interphase and mitosis as a result of localizing ERK1/2-MEK1 complexes to their tubulin-associated substrates.
The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrn1 in distinct cytoplasmic foci (2002)

Sm and Sm-like (LSm) proteins form heptameric complexes that are involved in various steps of RNA metabolism. In yeast, the Lsm1-7 complex functions in mRNA degradation and is associated with several enzymes of this pathway, while the complex LSm2-8, the composition of which largely overlaps with that of LSm1-7, has a role in pre-mRNA splicing. A human gene encoding an LSm1 homolog has been identified, but its role in mRNA degradation has yet to be elucidated. We performed subcellular localization studies and found hLSm1 predominantly in the cytoplasm. However, it is not distributed evenly; rather, it is highly enriched in small, discrete foci. The endogenous hLSm4 is similarly localized, as are the overexpressed proteins hLSm1-7, but not hLSm8. The foci also contain two key factors in mRNA degradation, namely the decapping enzyme hDcp1/2 and the exonuclease hXrn1. Moreover, coexpression of wild-type and mutant LSm proteins, as well as fluorescence resonance energy transfer (FRET) studies, indicate that the mammalian proteins hLSm1-7 form a complex similar to the one found in yeast, and that complex formation is required for enrichment of the proteins in the cytoplasmic foci. Therefore, the foci contain a partially or fully assembled machinery for the degradation of mRNA.
Drop drying on surfaces determines chemical reactivity - the specific case of immobilization of oligonucleotides on microarrays (2013)

Our experiments identified two basic processes determining immobilization. First, the rate of drop drying that depends on the drop volume and the ambient relative humidity. Oligonucleotides in a dried spot react unspecifically with the surface and long reaction times are needed. 3D hydrogel surfaces allow for immobilization in a liquid environment under diffusive conditions. Here, oligonucleotide immobilization is much faster and a specific reaction with the reactive linker group is observed. Second, the effect of increasing probe concentration as a result of drop drying. On a 3D hydrogel, the increasing concentration of probe molecules in nL spotting volumes accelerates immobilization dramatically. In case of μL volumes, immobilization depends on whether the drop is allowed to dry completely. At non-drying conditions, very limited immobilization is observed due to the low oligonucleotide concentration used in microarray spotting solutions. The results of our study provide a general guideline for microarray assay development. They allow for the initial definition and further optimization of reaction conditions for the immobilization of oligonucleotides and other probe molecule classes to different surfaces in dependence of the applied spotting and reaction volume.
Routine Quality Control of Clinical Nuclear Medicine Instrumentation: A Brief Review (2008)

This article reviews routine quality-control (QC) procedures for current nuclear medicine instrumentation, including the survey meter, dose calibrator, well counter, intraoperative probe, organ ("thyroid") uptake probe, gamma-camera, SPECT and SPECT/CT scanner, and PET and PET/CT scanner. It should be particularly useful for residents, fellows, and other trainees in nuclear medicine, nuclear cardiology, and radiology. The procedures described and their respective frequencies are presented only as general guidelines.
Optimal Parameters for Maneuverability of Affordable Precision Munitions (2012)

High maneuverability of guided projectiles enables engagement of fleeing targets, opens the area of influence of a weapon system, and allows new missions to be performed such as prosecuting targets in defilade. Gun-launched precision munitions have unique constraints that create technical barriers to achieving enhanced maneuverability. Structural integrity during the gun launch event, packaging control surfaces within the launch tube, and affordability are paramount concerns. The present work is a fundamental investigation of the flight mechanics and guidance, navigation, and control technologies necessary to optimize maneuverability of affordable precision projectiles. Detailed aerodynamic modeling and nonlinear equations of motion for the flight of a fin-stabilized airframe meeting low control authority constraints were implemented in simulation. Flight control laws were developed for various maneuver schemes with different actuator realizations. Simulations were conducted over a large parameter space to evaluate maneuverability. Results provide the optimal parameters within the distinctive scope of gun-launched munitions. Flying a skid-to-turn airframe with four canards in the “X” configuration maximizes control authority with moderate volume allocation and actuator bandwidth requirements. Examination of dynamic stability along with static stability illustrates that high-fidelity aerodynamic characterizations are required when optimizing maneuverability due to the implications on the airframe design and flight control algorithm development.
Spin Trapping of Free Radicals Produced in vivo in Heart and Liver During Endotoxemia (1989)

Studies using free radical scavengers and measurements of lipid peroxidation have suggested that free radicals are generated during endotoxemia. Conclusions from these studies have implied that free radicals may participate in the sequence of pathologic events following endotoxin challenge in the experimental animal. Current inferences of free radical generation and involvement have been derived from indirect evidence and are therefore inconclusive. To quantitate the generation of free radicals in vivo during endotoxemia this study employed the use of electron paramagnetic resonance spectroscopy (EPR) combined with spin trapping techniques. Five minutes before intraperitoneal endotoxin administration, trimethoxy-a-phenyl-t-butyl-nitrone [(MeO)3 PBN] was administered intraperitoneally. Experimental animals were always matched with control animals receiving no endotoxin. At either five minutes or twenty-five minutes following endotoxin administration animals were decapitated and hearts and livers were rapidly taken for lipid extraction and EPR evaluation. Analysis of the EPR spectra revealed hyperfine splitting constants that indicated the presence of carbon-centered radical spin adducts in both organ tissues from animals exposed to endotoxin for twenty-five minutes. No signals were present in hearts and livers taken five minutes after endotoxin administration. EPR evaluation did not indicate spin adduct formation in control tissue. These data directly demonstrate that activation of processes in vivo involving free radical generation occur early during endotoxemia, but are not detectable immediately after the endotoxin challenge.
Pedestrian Tracking Based on Colour and Spatial Information (2005)

This paper describes a tracking with appearance modelling system for pedestrians. A cascade of boosted classifiers and Haar-like rectangular features [6, 12] are used for the pedestrian detection. Statistical modelling in the HSV colour space is used for adaptive background modelling and subtraction, where the use of circular statistics for hue is proposed. By using the background model in combination with the detector, the system extracts a feature vector based on colour statistics and the spatial information. Circular [9] and linear statistics are applied on the extracted features to robustly track the pedestrians and other moving objects through the scene. An adaptive appearance model copes with partial or full occlusions and addresses the problem of missing or wrong detections in single frames.
A 2D-nmr Structure Determination of Spirasine X, a New Diterpene Alkaloid from Spiraea japonica (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA 2D-nmr Structure Determination of Spirasine X, a New Diterpene Alkaloid from Spiraea japonicaF. Sun, X. T. Liang, and D. Q. YuCite this: J. Nat. Prod. 1987, 50, 5, 923–926Publication Date (Print):September 1, 1987Publication History Published online1 July 2004Published inissue 1 September 1987https://doi.org/10.1021/np50053a026Request reuse permissionsArticle Views143Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (194 KB) Get e-Alertsclose Get e-Alerts
The Scunthorpe Bowl (1941)

An abstract is not available for this content so a preview has been provided. Please use the Get access link above for information on how to access this content.
A theoretical calculation of the piezoresistivity and magnetoresistivity in p-type semiconducting diamond films (2002)

A theoretical study of the piezoresistivity and magnetoresistivity in p-type heteroepitaxial diamond films is presented, based on the Fuchs-Sondheimer thin-film theory and the valence band split-off model. The Boltzmann transport equation in the relaxation time approximation was solved and a mixed scattering by lattice vibrations, ionized impurities and surfaces was considered. The analytical expressions for the piezoresistive and magnetoresistive effects has been developed in a parallel-connection resistance model for the light-hole, the heavy-hole and the split-off bands. The calculated results are discussed and compared with experimental data. It is found that the self-spin interaction of the heavy holes could be related to stress. Moreover, a possible mechanism is presented to explain the fact that the magnetoresistivity of the p-type heteroepitaxial diamond films is greater than that of the diamond bulk.
Mining the malignant ascites proteome for pancreatic cancer biomarkers (2011)

Pancreatic cancer (PC) is one of the most lethal malignancies and disease-specific biomarkers are desperately needed for better diagnosis, prognosis, monitoring treatment efficacy and for accelerating the development of novel targeted therapeutics. Being an advanced stage manifestation and a proximal fluid in contact with cancer tissues, the ascitic fluid presents itself as a promising rich source of biomarkers. Herein, we present a comprehensive proteomic analysis of pancreatic ascitic fluid. To fractionate the complex ascites proteome, we adopted a multi-dimensional chromatographic approach that included size-exclusion, ion-exchange and lectin-affinity chromatographic techniques. Our detailed proteomic analysis with high-resolution Orbitrap(®) mass spectrometer resulted in the identification of 816 proteins. We followed rigorous filtering criteria that consisted of PC-specific information obtained from three publicly available databases (Oncomine, Protein Atlas and Unigene) to segregate 20 putative biomarker candidates for future validation. Since these proteins are of membranous and extra-cellular origin, most are glycosylated, and many of them are over-expressed in cancer tissues, the probability of these proteins entering the peripheral blood circulation is high. Their validation as serological PC biomarkers in the future is highly warranted.