gebhart/patrepeval huggingface dataset:
<i>In Vivo</i> Gene Delivery and Expression of Physiological Levels of Functional Human Factor VIII in Mice (1995)

Hemophilia A is caused by blood coagulation factor VIII (FVIII) deficiency and is an attractive target for gene therapy. However, features of FVIII physiology, such as the instability of the mRNA and protein, have provided obstacles to the design of a feasible strategy for the transfer and expression of the human FVIII gene in vivo. We have constructed a recombinant adenoviral vector, Av1ALH81, that contains the human FVIII cDNA from which the B-domain has been deleted (BDD FVIII) and extensively characterized this vector in vitro and in vivo. In vitro, HepG2, human hepatoma cells, transduced with Av1ALH81 secreted high levels of biologically active human BDD FVIII measured by the Coatest bioassay (> 2,400 mU per 10(6) cells per 24 hr). Administration of Av1ALH81 to mice, via tail vein, resulted in expression of human BDD FVIII in the mouse plasma at levels averaging 307 +/- 93 ng/ml 1 week post-injection, measured by a sensitive human FVIII-specific ELISA. Normal FVIII levels in humans are 100-200 ng/ml, and therapeutic levels are as low as 10 ng/ml. Purification of the human FVIII from the mouse plasma, and subsequent Coatest analysis, revealed that the human FVIII produced in the mice was biologically active. In addition, the duration of FVIII expression in vivo was followed, and high-level FVIII expression was sustained over a period of several weeks. The finding that an adenoviral vector can mediate high-level expression of human FVIII in an animal model provides the basis for the development of gene therapy for hemophilia A.
Effect of manuka honey on the expression of universal stress protein A in meticillin-resistant Staphylococcus aureus (2011)

Staphylococcus aureus is an important pathogen that can cause many problems, from impetigo to endocarditis. With its continued resistance to multiple antibiotics, S. aureus remains a serious health threat. Honey has been used to eradicate meticillin-resistant S. aureus (MRSA) strains from wounds, but its mode of action is not yet understood. Proteomics provides a potent group of techniques that can be used to analyse differences in protein expression between untreated bacterial cells and those treated with inhibitory concentrations of manuka honey. In this study, two-dimensional (2D) electrophoresis was combined with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to determine the identities of proteins whose levels of expression were changed at least two-fold following treatment with manuka honey. Protein extracts were obtained from cells grown in tryptone soy broth (with or without manuka honey) by mechanical disruption and were separated on 2D polyacrylamide gels. A protein was isolated in gels prepared from untreated cell extract that was absent from gels made using honey-treated cell extract. Using MALDI-TOF MS, the protein was identified as universal stress protein A (UspA). Downregulation of this protein was confirmed by real-time polymerase chain reaction (PCR), which showed a 16-fold downregulation in honey-treated cells compared with untreated samples. This protein is involved in the stress stamina response and its downregulation could help to explain the inhibition of MRSA by manuka honey.
Interfacial tensions between molten magnesium and salts of the magnesium chloride-potassium chloride-barium chloride system (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInterfacial tensions between molten magnesium and salts of the magnesium chloride-potassium chloride-barium chloride systemJohn N. RedingCite this: J. Chem. Eng. Data 1971, 16, 2, 190–195Publication Date (Print):April 1, 1971Publication History Published online1 May 2002Published inissue 1 April 1971https://pubs.acs.org/doi/10.1021/je60049a007https://doi.org/10.1021/je60049a007research-articleACS PublicationsRequest reuse permissionsArticle Views100Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Temperature- and pH-dependent changes in the coordination sphere of the heme c group in the model peroxidase N alpha-acetyl microperoxidase-8. (1992)

The pH- and temperature-dependent changes in the coordination sphere of the heme c group of N alpha-acetyl microperoxidase-8 (Ac-MP-8) have been studied by examining its optical, resonance Raman, electron paramagnetic resonance, and magnetic circular dichroism spectra. An optical titration indicates that Ac-MP-8 exists in three major ionization forms over the pH 1-12 range that are linked by pK alpha values of approximately 3 and 9. The acid form that is present at pH 1.5 exists as a mixture of five- and six-coordinate high-spin species and most likely has water or buffer ions as axial ligand(s). On titration to pH 7, the His18 residue is deprotonated and becomes the proximal ligand to the iron to give a six-coordinate neutral form that has water as the sixth ligand. This form exists in a thermal high-spin intermediate-spin state equilibrium. On raising the pH to 10, an alkaline form is generated which is predominantly a five-coordinate high-spin species. It is formed by ionization of the proximal His18 residue to its imidazolate form with concomitant dissociation of the water ligand at the sixth site. At concentrations of Ac-MP-8 greater than 10 microM, some six-coordinate low-spin species are formed that are attributed to a dimer in which a His18 residue from a second molecule of Ac-MP-8 coordinates to the sixth site of another to give a bis-His complex. Raising the pH to 11.5 does not produce an appreciable amount of the six-coordinate complex with hydroxide as the sixth ligand. These studies show that Ac-MP-8 is a good water-soluble model for the peroxidases that exhibits minimal aggregation at concentrations below 10 microM in the neutral and alkaline pH regions.
Phylogeny and character evolution in <i>Medicago</i> (Leguminosae): Evidence from analyses of plastid <i>trnK/matK</i> and nuclear <i>GA3ox1</i> sequences (2010)

These results support the hypothesis that some characters considered important in existing taxonomies, for example, single-seeded fruits that have arisen more than once in both Medicago and Trigonella, are indeed homoplastic. Others, such as the 2n = 14 chromosome number, have also arisen independently within the genus. In addition, we demonstrate support for the utility of GA3ox1 sequences for phylogenetic analysis among and within closely related genera of legumes.
Joining ionics and electronics: semiconductor chips with ion channels, nerve cells, and brain tissue (2005)

The microscopic interfacing of semiconductor devices and living neuronal systems is discussed. From the biological side, ion channels, nerve cells, and brain tissue are considered. Basic experimental studies are performed with capacitors and transistors of simple silicon chips. The approach is extended to a CMOS chip with 16000 transistor sensors.
Effects of Hebeloma arenosa on growth and survival of container-grown red pine seedlings (Pinus resinosa) (1991)

The ability of the ectomycorrhizal fungus Hebelomaarenosa Burdsall, MacFall & Albers to enhance growth and survival of container-grown red pine seedlings (Pinusresinosa Ait.) was investigated. Shoot height of H. arenosa inoculated seedlings was 28% greater than noninoculated seedlings when grown without fertilizer applications. Eight-week-old seedlings transplanted into a Ball mix (a mixture of peat, bark, and perlite) containing up to a 1:64 dilution of fungal inoculum had significantly greater root dry weights and root/shoot ratios than noninoculated seedlings when grown for an additional 14 weeks. Under experimental greenhouse conditions, root and shoot dry weights of container-grown red pine seedlings that had been directly seeded into Ball mix containing up to a 1:256 dilution of fungal inoculum were significantly greater than weights measured for noninoculated seedlings. Root and shoot dry weights of container-grown seedlings seeded directly into a 1:5 dilution of H. arenosa inoculum and Ball mix and then grown under commercial production conditions were greater than comparable weights of noninoculated seedlings. Hebelomaarenosa inoculation significantly increased seedling survival following outplanting, but did not increase seedling growth. Hebelomaarenosa did not colonize roots growing from the root plug into the surrounding soil.
Small Molecule Blockers of Voltage‐gated Sodium Channels (2006)

This chapter contains sections titled: Drugs that Act on Sodium New Insights for Launched Compounds Challenges of Current Agents Compounds in Clinical Development New Blockers in Discovery or Pre-clinical Stage Emerging Indications and Future Directions
Prediction of sleep‐disordered breathing by unattended overnight oximetry (1999)

Summary Between January 1994 and July 1997, 793 patients suspected of having sleep‐disordered breathing had unattended overnight oximetry in their homes followed by laboratory polysomnography. From the oximetry data we extracted cumulative percentage time at S a O 2 < 90% (CT 90 ) and a saturation variability index (ΔIndex, the sum of the differences between successive readings divided by the number of readings – 1). CT 90 was weakly correlated with polysomnographic apnea/hypopnea index (AHI), (Spearman 𝓅 =0.36, P < 0.0001) and with ΔIndex ( 𝓅 =0.71, P < 0.0001). ΔIndex was more closely correlated with AHI ( 𝓅 =0.59, P < 0.0001). In a multivariate model, only ΔIndex was significantly related to AHI, the relationship being AHI=18.8 ΔIndex +7.7. The 95% CI for the coefficient were 16.2, 21.4, and for the constant were 5.8, 9.7. The sensitivity of a ΔIndex cut‐off of 0.4 for the detection of AHI≥15 was 88%, for detection of AHI≥20 was 90% and for the detection of AHI≥25 was 91%. The specificity of ΔIndex ≥0.4 for AHI≥15 was 40%. In 113 further patients, oximetry was performed simultaneously with laboratory polysomnography. Under these circumstances ΔIndex was more closely correlated with AHI ( 𝓅 =0.74, P < 0.0001), as was CT 90 ( 𝓅 =0.58, P < 0.0001). Sensitivity of ΔIndex ≥0.4 for detection of AHI≥15 was not improved at 88%, but specificity was better at 70%. We concluded that oximetry using a saturation variability index is sensitive but nonspecific for the detection of obstructive sleep apnea, and that few false negative but a significant proportion of false positive results arise from night‐to‐night variability.
The Yeast <i>SEN3</i> Gene Encodes a Regulatory Subunit of the 26S Proteasome Complex Required for Ubiquitin-Dependent Protein Degradation In Vivo (1995)

The yeast Sen1 protein was discovered by virtue of its role in tRNA splicing in vitro. To help determine the role of Sen1 in vivo, we attempted to overexpress the protein in yeast cells. However, cells with a high-copy SEN1-bearing plasmid, although expressing elevated amounts of SEN1 mRNA, show little increase in the level of the encoded protein, indicating that a posttranscriptional mechanism limits SEN1 expression. This control depends on an amino-terminal element of Sen1. Using a genetic selection for mutants with increased expression of Sen1-derived fusion proteins, we identified mutations in a novel gene, designated SEN3. SEN3 is essential and encodes a 945-residue protein with sequence similarity to a subunit of an activator of the 20S proteasome from bovine erythrocytes, called PA700. Earlier work indicated that the 20S proteasome associates with a multisubunit regulatory factor, resulting in a 26S proteasome complex that degrades substrates of the ubiquitin system. Mutant sen3-1 cells have severe defects in the degradation of such substrates and accumulate ubiquitin-protein conjugates. Most importantly, we show biochemically that Sen3 is a subunit of the 26S proteasome. These data provide evidence for the involvement of the 26S proteasome in the degradation of ubiquitinated proteins in vivo and for a close relationship between PA700 and the regulatory complexes within the 26S proteasome, and they directly demonstrate that Sen3 is a component of the yeast 26S proteasome.
Dementia and Alzheimer's disease. Indications, diagnosis, and treatment. (1995)

At least 6 million Americans suffer from some form of dementia. More than half of those patients are afflicted with Alzheimer's disease, a progressive, degenerative neurological disorder. Reaching the diagnosis of Alzheimer's, however, is often difficult since Alzheimer's can be accurately diagnosed only after all other options are ruled out. The process is painstaking, but it's also the crucial link in ensuring proper diagnosis and treatment. There's no cure yet for Alzheimer's, but researchers are narrowing in on possible causes of the disease and identifying patients who may be predisposed to developing it. Some of the most compelling research focuses on the presence of one or more of the apolipoprotein E4 genes. Researchers also continue to develop new drugs and physical therapies. In the Twin Cities, several drug studies are underway, and community resources are available to provide Alzheimer's patients and their families with information on everything from support groups to assisted-living arrangements.
Environmentally Clean Mitigation of Undesirable Plant Life Using Lasers (2009)

This concept comprises a method for environmentally clean destruction of undesirable plant life using visible or infrared radiation. We believe that during the blossom stage, plant life is very sensitive to electromagnetic radiation, with an enhanced sensitivity to specific spectral ranges. Small doses of irradiation can arrest further plant growth, cause flower destruction or promote plant death. Surrounding plants, which are not in the blossoming stage, should not be affected. Our proposed mechanism to initiate this effect is radiation produced by a laser. Tender parts of the blossom possess enhanced absorptivity in some spectral ranges. This absorption can increase the local tissue temperature by several degrees, which is sufficient to induce bio-tissue damage. In some instances, the radiation may actually stimulate plant growth, as an alternative for use in increased crop production. This would be dependent on factors such as plant type, the wavelength of the laser radiation being used and the amount of the radiation dose. Practical, economically viable realization of this concept is possible today with the advent of high efficiency, compact and powerful laser diodes. The laser diodes provide an efficient, environmentally clean source of radiation at a variety of power levels and radiation wavelengths. Figure 1 shows the overall concept, with the laser diodes mounted on a movable platform, traversing and directing the laser radiation over a field of opium poppies.
LAMP‐2‐deficient human B cells exhibit altered MHC class II presentation of exogenous antigens (2010)

Major histocompatibility complex (MHC) class II molecules present antigenic peptides derived from engulfed exogenous proteins to CD4(+) T cells. Exogenous antigens are processed in mature endosomes and lysosomes where acidic proteases reside and peptide-binding to class II alleles is favoured. Hence, maintenance of the microenvironment within these organelles is probably central to efficient MHC class II-mediated antigen presentation. Lysosome-associated membrane proteins such as LAMP-2 reside in mature endosomes and lysosomes, yet their role in exogenous antigen presentation pathways remains untested. In this study, human B cells lacking LAMP-2 were examined for changes in MHC class II-restricted antigen presentation. MHC class II presentation of exogenous antigen and peptides to CD4(+) T cells was impaired in the LAMP-2-deficient B cells. Peptide-binding to MHC class II on LAMP-2-deficient B cells was reduced at physiological pH compared with wild-type cells. However, peptide-binding and class II-restricted antigen presentation were restored by incubation of LAMP-2-negative B cells at acidic pH, suggesting that efficient loading of exogenous epitopes by MHC class II molecules is dependent upon LAMP-2 expression in B cells. Interestingly, class II presentation of an epitope derived from an endogenous transmembrane protein was detected using LAMP-2-deficient B cells. Consequently, LAMP-2 may control the repertoire of peptides displayed by MHC class II molecules on B cells and influence the balance between endogenous and exogenous antigen presentation.
Video Denoising Based on Adaptive Temporal Averaging (2012)

Abstract: This paper proposes a video denoising algorithm based on adaptive, pixel-wise, temporal averaging. The algorithm decomposes videos into a set of 1-D time dependent signals and then removes the noise by establishing temporal averaging intervals throughout each signal from the set. Temporal averaging intervals are established by simple, yet effective comparison processes which include two-way thresholding. The proposed algorithm is tested on several types of 1-D signals and benchmark videos. Experiments suggest that the proposed algorithm, despite its simplicity, produces high-quality denoising results and even outperforms some state-of-the-art competitors.
Performances of a Linear Type Magnetic Flux Pump for Compensating a Little Decremented Persistent Current of HTS Magnets (2004)

This paper describes the characteristics of a linear type magnetic flux pump recently developed. The linear type flux pump has been fabricated to apply to compensate decremented persistent current of HTS applications such as NMR and MRI system. The linear type magnetic flux pump mainly consists of 3-phase AC armature coil, DC bias coil and superconducting Nb foil. In the preliminary experiment, the flux pump can charge the pumping-up current in the load coil of 1.3 mH during 1000 seconds under the DC bias current of 10 A and 3-phase AC of 8 A, 60 Hz. The magnitude of pumping current is 0.1 mA/s.
A 2-V 10.7-MHz CMOS limiting amplifier/RSSI (2000)

This paper presents low-voltage low-power CMOS circuit design techniques for an intermediate frequency (IF) limiting amplifier and received signal strength indicator (RSSI). The architecture of the limiting amplifier and RSSI employed is determined by the optimal power consumption for a specified speed, overall gain, and accuracy. Each gain cell of the limiting amplifier employs folded diode load for low-voltage operation. Offset is reduced by a cross-connected source-coupled pair offset subtractor that is along the signal path. Full-wave current rectification and summation are employed in the RSSI circuit to achieve high precision while maintaining low voltage and low power. Using a single 2-V supply voltage, measured results demonstrate the input dynamic range is larger than 75 dB for 10.7-MHz IF application. The prototype occupies an active area of 0.4 mm/sup 2/ using a 0.6-/spl mu/m digital CMOS technology. The power dissipation is 6.2 mW.
Comments on Calibration and Design of a Heat Flow Meter (1983)

After a review of the calibration of two heat flow meter (HFM) transducers, the HFM has been redesigned. The new design produces a calibration independent of the characteristics of the material being tested; that is, it produces the same calibration curve for thick, low-density specimens as for thin, high-density ones. Calibration within ±1 percent of transfer standard accuracy is discussed; the HFM precision for thick specimens of low-density glass fiber appears to be higher than that of a guarded hot plate under the same testing conditions.
Lanthanide-Based Lamellar Nanohybrids:  Synthesis, Structural Characterization, and Optical Properties (2006)

A general nonaqueous route has been applied for the preparation of lanthanide ordered nanocrystalline hybrid structures. In a simple one-pot reaction process, Ln(III) isopropoxides (Ln = Gd, Sm, Nd) were dissolved in benzyl alcohol and reacted in an autoclave between 250 and 300 °C. This approach leads to crystalline lanthanide oxide layers regularly separated from each other by organic layers of intercalated benzoate molecules. They display good thermal stability for temperature up to 400 °C. The gadolinium-based nanohybrids showed outstanding optical emission properties when doped with terbium(III) and europium(III).
Pulsed plasma-pulsed injection sources for remote plasma activated chemical vapor deposition (1993)

Remote plasma activated chemical vapor deposition (RPACVD) is an attractive fabrication technique owing to the increased selectivity of radical generation which can be obtained compared to deposition techniques in which the substrate is immersed in the plasma. This selectivity can be compromised if the deposition gases, which are typically injected downstream of the plasma zone, back-diffuse into the plasma where indiscriminate electron impact dissociation occurs. In this communication, a new RPACVD technique is described in which the plasma and injected gases are sequentially pulsed to temporally isolate the injected gases from the plasma. This method reduces, or eliminates, indiscriminate dissociation of the injected gases and improves the selectivity of radical fluxes to the substrate.