gebhart/patrepeval huggingface dataset:
Trm1p, a Zn(II) <sub>2</sub> Cys <sub>6</sub> -Type Transcription Factor, Is a Master Regulator of Methanol-Specific Gene Activation in the Methylotrophic Yeast <i>Candida boidinii</i> (2008)

The methylotrophic yeasts are commonly used as hosts for heterologous gene expression. In this study, we describe a novel gene, TRM1, in Candida boidinii, responsible for the transcriptional activation of several methanol-inducible promoters. The encoded protein, Trm1p, is a Zn(II)2Cys6-type zinc cluster protein. Deletion of TRM1 completely inhibits growth on methanol but causes no growth defect on glucose or other nonfermentative carbon sources, glycerol, ethanol, or oleate. Trm1p is responsible for transcriptional activation of five methanol-inducible promoters tested, but not for peroxisome assembly or peroxisomal protein transport. Expression of the TRM1 gene was constitutive, and Trm1p localizes to the nuclei regardless of the carbon source. Two cis-acting methanol response elements (MREs), MRE1 and MRE2 are present in the promoter of the dihydroxyacetone synthase gene. Trm1p is shown to be required for MRE1-dependent methanol-inducible gene expression. Chromatin immunoprecipitation assays reveal that Trm1p binds to five methanol-inducible promoters upon methanol induction but does not bind in glucose-grown cells. Thus, the TRM1 gene encodes a master transcriptional regulator responsible for methanol-specific gene activation in the methylotrophic yeasts.
Si wire waveguide polarisation‐independent wavelength filter using polarisation rotation Bragg grating (2014)

A polarisation‐independent Bragg grating wavelength filter using polarisation rotation is reported. A rib waveguide Bragg grating structure and second‐order mode are used to generate the polarisation rotation. A device connected to polarisation beam splitter (PBS) to obtain a polarisation‐independent device is fabricated. The measured diffraction wavelength peaks were almost the same for both input polarisations.
Upstream activation and repression elements control transcription of the yeast COX5b gene. (1990)

The Saccharomyces cerevisiae COX5b gene is regulated at the level of transcription by both the carbon source and oxygen. To define the cis-acting elements that underlie this transcriptional control, deletion analysis of the upstream regulatory region of COX5b was performed. The results of the study suggest that at least four distinct regulatory sites are functional upstream of the COX5b transcriptional starts. One, which was precisely defined to a region of 20 base pairs, contains two TATA-like elements. Two upstream activating sequences (UAS15b and UAS2(5b)) and an upstream repression sequence (URS5b) were also found. Each of the latter three elements was able either to activate (UAS1(5b) and UAS2(5b)) or to repress URS5b) the transcription of a heterologous yeast gene. Further analysis revealed that UAS1(5b) is the site of carbon source control and may be composed of two distinct domains that act synergistically. URS5b mediates the aerobic repression of COX5b and contains two sequences that are highly conserved in other yeast genes negatively regulated by oxygen.
Decreased Asialotransferrin in Cerebrospinal Fluid of Patients with Childhood-Onset Ataxia and Central Nervous System Hypomyelination/Vanishing White Matter Disease (2005)

The transferrin isoform abnormality in the cerebrospinal fluid of patients with CACH/VWM appears unique and is a potential clinical diagnostic biomarker. The rapid, efficient diagnosis of this disorder would have a significant impact on clinical studies exploring new strategies for the management and treatment of this disease.
Multipass Michelson interferometer with the use of a wavelength-modulated laser diode (1996)

An improved multipass Michelson interferometer is implemented. This technique uses the fact that the wavelength of a laser diode varies in proportion to the diode's injection current. With this method the sensitivity augmentation is accomplished by inserting a beam splitter into one arm of the interferometer, resulting in multiple reflections between the end mirror and the beam splitter. In addition, the interference of laser beams reflected from two arms can be accomplished with unequal arms in the condition of a short coherence length. The sensitivity increase of interference fringes and the compensation of the short coherence length have been demonstrated in experiments.
Topiramate and Lamotrigine Pharmacokinetics during Repetitive Monotherapy and Combination Therapy in Epilepsy Patients (2003)

The results of this study show that no PK interaction between TPM and LTGwas observed at the doses used in this study.
Successful Alloy Attachment of GaAs MMIC's (1987)

Alloy attachment of GaAs monolithic circuits was examined after initial reflow, after environmental bake, and after a stepped series of thermal cycles from 200 to 1000 cycles (-55 to + 125°C). The variety of solders tested included both pastes and preforms. Monolithic assemblies alloyed with these solders were evaluated for changes in physical properties as well as for changes in electrical performance. It was noted during the study that via fractures due to thermal expansion differences between the alloy and the GaAs monolithic device were a common occurrence and could become an inherent reliability risk. Based on this evidence, an investigation relating the frequency of fracturing to the size and the shape of the vias was undertaken. Results led to the development of processing parameters which could minimize and control fracture occurrence.
Experimental Shock with Special Reference to Anesthesia (1934)

No other journal can match Anesthesia & Analgesia for its original and significant contributions to the anesthesiology field. Each monthly issue features peer-reviewed articles reporting on the latest advances in drugs, preoperative preparation, patient monitoring, pain management, pathophysiology, and many other timely topics. Backed by internationally-known authorities who serve on the Editorial Board and as Section Editors, Anesthesia &Analgesia is your gateway to everything that is happening in anesthesia and 14 related subspecialties: Analgesia; Ambulatory Anesthesia; Anesthetic Pharmacology; Cardiovascular Anesthesia; Critical Care and Trauma; Economics, Education, and Policy; Neurosurgical Anesthesia; Obstetric Anesthesia; Pain Mechanisms; Pain Medicine; Pediatric Anesthesia; Regional Anesthesia; Patient Safety; and Technology, Computing and Simulation.
REGULATION OF THYROTROPIN-RELEASING HORMONE RECEPTORS IS CELL TYPE SPECIFIC: COMPARISON OF ENDOGENOUS PITUITARY RECEPTORS AND RECEPTORS TRANSFECTED INTO NON-PITUITARY CELLS (1991)

TRH, which does not elevate cyclic AMP, and elevation of cellular cyclic AMP decrease the density (down-regulate) of TRH receptors (TRH-Rs) on pituitary (GH3) cells. In this study we measured the effects of TRH and elevation of cyclic AMP on TRH-Rs expressed in non-pituitary cells transfected with a recently cloned mouse pituitary TRH-R complementary DNA. In stably transfected rat glioma (C6-2) cells and transiently transfected COS-1 cells TRH caused TRH-R down-regulation while elevation of cyclic AMP caused increases in TRH-R density. Hence, the effects of cyclic AMP on TRH-Rs in transfected C6-2 and COS-1 cells are different from those in GH3 cells while the effects of TRH on TRH-R are similar in all three cell types. These data show that regulation of TRH-Rs is cell type specific.
Distributed heterogeneous information systems and schema transformation (2002)

The advantages of the object-oriented data model Cosmos as a conceptual modeling facility for distributed database architectures are highlighted. It is shown that this data model not only offers the possibility of defining highly structured abstract constructs but also provides a general-purpose programming environment for the definition of additional operations upon already existing heterogeneous data. The problem of extracting information from existing heterogeneous data representations is also addressed, and the use of a two-layered approach is suggested. In the two-layered quintessence model architecture the user can utilize highly structured objects at the upper layer, while all interschema and interlanguage transformations are performed in a transparent manner at the lower layer. A two-layered model configuration is introduced for the case of semidecentralized distributed heterogeneous information systems and it is explained how its lower layer is in a position to map heterogeneous host schemas to each other.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Symbol synchronization in convolutionally coded systems (Corresp.) (1979)

Alternate symbol inversion is sometimes applied to the output of convolutional encoders to guarantee sufficient richness of symbol transition for the receiver symbol synchronizer. A bound is given for the length of the transition-free symbol stream in such systems, and those convolutional codes are characterized in which arbitrarily long transition free runs occur.
Timing of Peripheral Nerve Repair: Important Local Neuropathological Factors (1977)

A review of the local neuropathological factors suggests that in lesions-in-continuity, the timing of surgical intervention should be predicated by the histological structure of that particular lesion-in-continuity. This cannot be derived by inspection or palpation of the lesion. The information must be gained from clinical and electrical studies. In the case of the divided nerve, the interrupted fascicles should be sutured as soon as the longitudinal extent of the injury can be accurately determined. The ultimate factor limiting the successful outcome of delayed peripheral nerve surgery is probably related to the state of the end organs and muscle fibers.
Fabrication and Characterization of PZT/Thermoplastic Polymer Composites for High‐Frequency Phased Linear Arrays (1994)

A new method of fabricating PZT/potymer composites with 2‐2 connectivity is described. This fabrication technique offers significant advantages over conventional dice‐and‐fill fabrication methods, and the composites exhibit the high electromechanical coupling expected from conventional PZT/polymer composites. In this method, thin (≤20μm) sintered PZT plates and sheets of a thermoplastic polymer film (≤6 μm) are bonded together via thermal processing. A technique for sintering thin, flat PZT plates from tape cast materials was developed to provide the necessary PZT plates. The resulting composite blocks were cut to required dimensions, electroded, and poled. Electromechanical properties were measured to evaluate the composites.
Fusion of Smartphone Motion Sensors for Physical Activity Recognition (2014)

For physical activity recognition, smartphone sensors, such as an accelerometer and a gyroscope, are being utilized in many research studies. So far, particularly, the accelerometer has been extensively studied. In a few recent studies, a combination of a gyroscope, a magnetometer (in a supporting role) and an accelerometer (in a lead role) has been used with the aim to improve the recognition performance. How and when are various motion sensors, which are available on a smartphone, best used for better recognition performance, either individually or in combination? This is yet to be explored. In order to investigate this question, in this paper, we explore how these various motion sensors behave in different situations in the activity recognition process. For this purpose, we designed a data collection experiment where ten participants performed seven different activities carrying smart phones at different positions. Based on the analysis of this data set, we show that these sensors, except the magnetometer, are each capable of taking the lead roles individually, depending on the type of activity being recognized, the body position, the used data features and the classification method employed (personalized or generalized). We also show that their combination only improves the overall recognition performance when their individual performances are not very high, so that there is room for performance improvement. We have made our data set and our data collection application publicly available, thereby making our experiments reproducible.
Liver transplantation in a patient with unresectable colorectal liver metastases -- a case report. (2014)

In patients with multiple unresectable liver onlycolorectal metastases, liver transplantation may improve overallsurvival and quality of life, by using marginal grafts whichcannot be allocated to the patients with standard indicationsfor liver transplantation. The advent of MDCT and PET CT scan and the use of m-TOR inhibitors may improve the resultsachieved by liver transplantation in patients with CLMs.Further studies could be useful in an attempt to disclosewhether a selected group of patients with unresectable liveronly colorectal metastases could become acceptable candidatesfor liver transplantation.
Checking Consistency of C and Verilog using Predicate Abstraction and Induction (2004)

It is common practice to write C models of circuits due to the greater simulation efficiency.Once the C program satisfies the requirements, the circuit is designed in a hardware description language (HDL) such as Verilog.It is therefore highly desirable to automatically perform a correspondence check between the C model and a circuit given in HDL.We present an algorithm that checks consistency between an ANSI-C program and a circuit given in Verilog using Predicate Abstraction.The algorithm exploits the fact that the C program and the circuit share many basic predicates.In contrast to existing tools that perform predicate abstraction, our approach is SAT-based and allows all ANSI-C and Verilog operators in the predicates.We report experimental results on an out-of-order RISC processor.We compare the performance of the new technique to Bounded Model Checking (BMC).
Transverse particle trajectories in high-gradient magnetic separation (1982)

We have used human red blood cells as a model particle system for studying transverse particle trajectories relevant to high gradient magnetic separators. Trajectories measured around a single 50 μm diameter wire are compared with calculations based on potential and laminar flow models of the fluid motion. The magnetic velocity is determined in an independent experiment which precisely models potential flow. At the low Reynolds numbers (Re = 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-3</sup> ) used here it is found that the laminar flow modeI gives better agreement with experiment.
An improved floating-random-walk algorithm for solving the multi-dielectric Dirichlet problem (1993)

An improved floating-random-walk algorithm for solving the multidielectric Dirichlet problem is outlined. The improvement is achieved by using statistically generated Green's functions that are calculated beforehand and stored as lookup tables. These tables have been used to solve the multidielectric Dirichlet problem for an arbitrary two-dimensional geometry. The improved algorithm is also compared with the conventional floating-random-walk algorithm and is found to be at least two times more efficient. Results are presented for two types of parallel-plate geometries.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Straightforward Synthesis of (1→2)-Linked Pseudo Aza-<i>C</i>-disaccharides by the Novel Cycloaddition of Enantiopure Cyclic Nitrones to Glycals (1998)

The novel, highly stereoselective, intermolecular cycloaddition reaction of enantiopure cyclic nitrones 8 to 1,2-glycals 9 opens the way to a straightforward synthesis of a broad class of new (1-->2)-linked pseudo aza-C-disaccharides 6, suitable substrates for selective inhibition of glycosidase enzymes. The cycloadditions occur with high stereocontrol, displaying preferential interaction between the bottom face of the glycal and the face of the nitrone anti to the substituent on C-3, with the reagents approaching in an exo fashion. The cycloadditions produced tricyclic isoxazolidines 7 that represent nonreducing pseudo aza-C-disaccharides stable to hydrolytic conditions. The target pseudo aza-disaccharides 6 were obtained by sequential deprotection of the hydroxyl groups and isoxazolidine ring-opening.
Coordinatively Saturated Tris(oxazolinyl)borato Zinc Hydride-Catalyzed Cross Dehydrocoupling of Silanes and Alcohols (2011)

The four-coordinate zinc compound ToMZnH (1, ToM = tris(4,4-dimethyl-2-oxazolinyl)phenylborate) catalyzes selective alcoholysis of substituted hydrosilanes. The catalytic reaction of PhMeSiH2 and aliphatic alcohols favors the monodehydrocoupled product PhMeHSi–OR. With the aryl alcohol 3,5-C6H3Me2OH, the selectivity for mono(aryloxy)hydrosilane PhMeHSiOC6H3Me2 and bis(aryloxy)silane PhMeSi(OC6H3Me2)2 is controlled by relative reagent concentrations. Reactions of secondary organosilanes and diols provide cyclic bis(oxo)silacycloalkanes in high yield. The empirical rate law for the ToMZnH-catalyzed reaction of 3,5-dimethylphenol and PhMeSiH2 is −d[PhMeSiH2]/dt = k′obs[ToMZnH]1[3,5-C6H3Me2OH]0[PhMeSiH2]1 (determined at 96 °C) which indicates that Si–O bond formation is turnover-limiting in the presence of excess phenol.
Novel and Direct Nucleophilic Sulfenylation and Thiocyanation of Phenol Ethers Using a Hypervalent Iodine(III) Reagent (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel and Direct Nucleophilic Sulfenylation and Thiocyanation of Phenol Ethers Using a Hypervalent Iodine(III) ReagentYasuyuki Kita, Takeshi Takada, Sachiko Mihara, Brendan A. Whelan, and Hirofumi TohmaCite this: J. Org. Chem. 1995, 60, 22, 7144–7148Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/jo00127a018https://doi.org/10.1021/jo00127a018research-articleACS PublicationsRequest reuse permissionsArticle Views1112Altmetric-Citations97LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Validation and Application of a Protocol for the Extraction and Quantitative Analysis of Sphingomyelin in Erythrocyte Membranes of Patients with NAFLD (2021)

ABSTRACT A set of constituents of the erythrocyte membrane lipidome has been proposed to serve as biomarkers for liver disease and acute coronary syndrome. In erythrocytes, sphingomyelin hydrolysis provides ceramide, a signaling lipid necessary for phosphatidylserine exposure and eryptosis. Phosphatidylserine exposure further amplifies hepatic inflammation and fibrosis during non-alcoholic fatty liver disease (NAFLD). In this study, we developed and applied a quantitative TLC for erythrocyte membrane sphingomyelin of NAFLD patients. We also compared 10 extraction methods for the isolation of sphingomyelin from erythrocytes. For quantitative TLC, lipids were separated in Silica gel 60 F254 using a mixture of chloroform/methanol/acetic acid/water (60/50/1/4) (v/v/v/v). The separated lipids were stained in a chamber containing iodine, and the intensity of each of the primary colors (red, green, blue) and the sum of the Red plus Green colors (R+G) was analyzed. The method was linear over a wide range of concentrations, presented acceptable precision (inter-day CV(%) 0.34, 0.006 and 0.44 for 2.5, 5.0 and 10 μg, respectively), good accuracy (recovery range 85.2-97.1%), and excellent limit of detection (0.137 μg/spot) and limit of quantification (0.41 μg/spot). Using this quantitation method, we compared various lipid extraction methods and found that lipid extraction with methanol led to higher yield of erythrocyte sphingomyelin (135.35±1.04% recovery, compared to the Folch method). Application of these methods showed that erythrocytes from NAFLD patients (9 men, 15 women, 57.95±11.11 years old) contained statistically significantly less sphingomyelin (829.82±511.60 vs 1892.08±606.25 μg/ml of packed erythrocytes) compared to healthy controls (4 men, 6 women, 39.3±15.55 years old).
Spontaneous hemothorax. Report of 6 cases and review of the literature. (1992)

We present 6 cases of spontaneous hemothorax and comprehensively review the medical literature on this subject. We categorize the reported causes and offer a rational diagnostic approach to patients with nontraumatic hemothorax. We recommend specific treatments for specific etiologies, and emphasize the importance of well-established surgical principles for the treatment of hemothorax. Our suggestions should enable physicians to accurately diagnose and expeditiously treat patients with spontaneous hemothorax.
Tele-micro-surgery system with intelligent user interface (2002)

This paper describes a master-slave type tele-micro-surgical system with an intelligent user interface. To enhance the accuracy and the rigidity of the slave-manipulator, multicircular guides were adopted for the rotational degrees of freedom. To increase the operability of the system, an "antishadow" technique was used and the 3D relative information was presented on a 2D display. Furthermore, the anti-shadow was processed using a real-time tracking vision system to guide the slave-manipulator to the target position automatically. It took approximately 25 minutes to suture a 1 mm diameter artificial micro-blood-vessel using the system. The carotid artery of a rat with a diameter of 0.3 mm was also successfully sutured.
Bragg confinement of carriers in a shallow quantum well (1991)

A novel Bragg confining structure is proposed and analyzed. The combination of a shallow quantum well with superlattice-Bragg reflectors makes it possible to tailor separately the eigen-energy, and the wave function of the Bragg confined state. Longer lifetimes and higher effective barriers than in previously proposed Bragg confining structures is possible with the present scheme. Photoluminescence at energies close to the highest direct bandgap of the material system in use is expected from the proposed structure.
Patterning damage in narrow trackwidth spin-valve sensors (2003)

Using a combination of electron-beam lithography and Ar ion milling, we have fabricated spin-valve sensors with widths ranging from 30 to 250 nm. Although the resistance of the sensors scales as expected with width, the giant magnetoresistance (GMR) ratio decreases with decreasing width, consistent with the presence of a region with negligible GMR at the edges of the sensors. Sensors patterned using a focused ion beam showed a similar but much greater effect. We attribute this behavior to edge damage associated with the patterning process.