gebhart/patrepeval huggingface dataset:
Novel Bombyx mori cell lines cultivable at 37&deg;C (1999)

Four continuous cell lines were established from cultures of embryonic tissue of the silkworm, Bombyx mori, and designated NISES-BoMo-MK, NISES-BoMo-KG, NISES-BoMo-DZ, and NISES-BoMo-OH. The cells were mostly spherical in shape, and could be cultured at 25°C to 37°C. The population doubling time of each cell line was not affected by the temperatures employed, being about one day at both 25°C and 37°C. The karyotype of the cells was typical of lepidopteran cell lines. The number of chromosomes was higher at 37°C than at 25°C. The activity of the enzymes, phosphoglucose isomerase, phosphoglucomutase, isocitrate dehydrogenase, and glucose-6-phosphate dehydrogenase extracted from the cells was higher at 37°C than at 25°C. None of these novel cell lines formed any polyhedra, even with high titer inoculation of Bombyx mori nuclear polyhedrosis virus.
Supercontinuum generation in dispersion engineered highly nonlinear (γ = 10 /W/m) As_2S_3 chalcogenide planar waveguide (2008)

We demonstrate supercontinuum generation in a highly nonlinear As(2)S(3) chalcogenide planar waveguide which is dispersion engineered to have anomalous dispersion at near-infrared wavelengths. This waveguide is 60 mm long with a cross-section of 2 mum by 870 nm, resulting in a nonlinear parameter of 10 /W/m and a dispersion of +29 ps/nm/km. Using pulses with a width of 610 fs and peak power of 68 W, we generate supercontinuum with a 30 dB bandwidth of 750 nm, in good agreement with theory.
Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis (2000)

Qualitative and quantitative differences were observed and measured between the normal and malignant part of each sample. Morphologically altered nuclei displayed densely labelled spots within faintly labelled areas whereas normal nuclei were darker and uniformly stained. Image analysis allowed calculation of the average integrated optical density of the nuclei in both types of tissues, demonstrating a constant and significantly lower intensity for the former type of cells.
Non-insulin-treated ICA positive and negative diabetics are equally insulin resistant. (1983)

Insulin action was assessed in 5 cytoplasmic islet cell antibody (ICA) positive non-diabetics, 8 ICA positive (type I) non-insulin-treated diabetics, 7 ICA negative insulin-treated diabetics by measurement of steady state plasma glucose (SSPG) levels during a combined intravenous infusion of propranolol, adrenaline, glucose and insulin. SPPG values of ICA positive and negative non-diabetics were similar and their combined value (4.0 +/- 0.5 mmol/l) was significantly lower (p less than 0.01 and less than 0.01) than those (11.5 +/- 1.9 and 11.3 +/- 2.2 mmol/l) of ICA positive and negative diabetics, indicating that both groups of diabetics were similarly insulin resistant. Similar correlations were observed between SSPG and HbA1 levels when considering all ICA positive subjects (r = 0.89, p less than 0.001) and all ICA negative subjects (r = 0.73, p less than 0.01). Conventional insulin treatment (2.6 months) in 4 ICA positive diabetics improved insulin action in each case with a reduction in mean SSPG concentration from 14.0 +/- 2.3 to 8.5 +/- 3.4 mmol/l. Thus, ICA positive and negative diabetics, of equivalent degree of carbohydrate intolerance, are equally insulin resistant. Insulin treatment may improve, but appears not to normalise, insulin action in ICA positive (type I) diabetics.
" ACTIVATION " ET " SAILLANCE " D'UNITÉS GRAMMATICALES. QUELLE PLACE POUR L'INTONATION DANS LA CONSTRUCTION DYNAMIQUE DU SENS ? (2009)

International audience
gamma-tubulin is a minus end-specific microtubule binding protein. (1995)

The role of microtubules in mediating chromosome segregation during mitosis is well-recognized. In addition, interphase cells depend upon a radial and uniform orientation of microtubules, which are intrinsically asymmetric polymers, for the directional transport of many cytoplasmic components and for the maintenance of the structural integrity of certain organelles. The slow growing minus ends of microtubules are linked to the centrosome ensuring extension of the fast growing plus ends toward the cell periphery. However, the molecular mechanism of this linkage is not clear. One hypothesis is that gamma-tubulin, located at the centrosome, binds to the minus ends of microtubules. To test this model, we synthesized radiolabeled gamma-tubulin in vitro. We demonstrate here biochemically a specific, saturable, and tight (Kd = 10(-10) M) interaction of gamma-tubulin and microtubule ends with a stoichiometry of 12.6 +/- 4.9 molecules of gamma-tubulin per microtubule. In addition, we designed an in vitro assay to visualize gamma-tubulin at the minus ends of axonemal microtubules. These data show that gamma-tubulin represents the first protein to bind microtubule minus ends and might be responsible for mediating the link between microtubules and the centrosome.
Reactions Involved in Bioluminescence Systems of Limpet ( <i>Latia neritoides</i> ) and Luminous Bacteria (1972)

Luminescence in Latia involves a specific flavoprotein enzyme ("luciferase"), which has a tightly bound flavin group constituting the light-emitter. The overall reaction includes oxidation of a specific substrate ("luciferin," an enol formate derivative of an aliphatic aldehyde), by 2 O(2) molecules, in the presence of a "purple protein" cofactor, yielding a ketone, HCOOH, CO(2), and light. In Achromobacter, a required aliphatic aldehyde, which is functionally equivalent to Latia luciferin, is oxidized to an acid containing the same hydrocarbon chain as the aldehyde; this reaction proceeds in the presence of bacterial luciferase and reduced flavin mononucleotide with a quantum yield of 0.17 + 0.1 photons per aldehyde molecule that is independent of aldehyde chain length from 9 to at least 14 carbons.
Density Behavior of Drilling Fluids During High Pressure High Temperature Drilling Operations (1998)

Abstract In this study, we present an analytical model for the density-pressure-temperature dependence for drilling fluids. This generalized equation of state is valid for oil- and water based drilling fluids and also for completion fluids. The model is compared to other models already proposed, and is found to represent the measured data more accurately than the other models. In the application of the model we study the transient density behavior during drilling operations.
Prevention of optics and resist contamination in 300-mm lithography: improvements in chemical air filtration (2001)

Atmospheric pressure deep UV lithography using fast chemically amplified photoresists will be the mainstay of semiconductor production into the foreseeable future. Airborne molecular contamination (AMC) in the form of bases and condensable organic and inorganic materials however, threaten both sensitive optics and modern resists thereby creating a host of yield limiting contamination issues. Past work by Kunz at MIT has described photo-induced organic contamination of lithographic optics as a significant concern in leading-edge lithography. Moreover, Kinkead and Ercken, and Kishkovich and Dean have published work on the impact of base contamination on CD uniformity in modern photoresists. Herein, the authors discuss solutions to control both optics and resist contamination in a single compact filter system for advanced lithography. The results of this work suggest that resist and optics contamination can be controlled as we enter the era of low K1 factor <150nm/300mm-device production.
Endoscope-aided cataract surgery in corneal opacity associated with aniridia (2005)

Uka, Junko MD; Minamoto, Atsushi MD; Hirayama, Tomoko MD; Adachi, Toru MD; Yamane, Ken MD; Mishima, Hiromu K. MD Author Information
Biochemical effects of unsaponifiable lipidic components of avocado and soya bean administered percutaneously on the connective tissue components of hairless rat skin (1979)

Synopsis The unsaponifiable lipidic fractions of avocado and soya bean were administered percutaneously, on the dorsal skin of hairless rats for 15 days, in a 5% sweet almond oil solution. This treatment produced a modification of dermal connective tissue components. The biochemical analysis showed an increase of soluble proteins, especially of salt soluble collagen together with a decrease of proteins insoluble in neutral buffer. The ratio, soluble proteins:insoluble proteins, increases as well as the ratio soluble collagen: insoluble collagen. These modifications can be considered as a result of the activation of connective tissue metabolism. The above results are in agreement with those obtained when the unsaponifiables were administered per os to rats. Effets biochimiques, sur le tissu conjonctif sous cutané des insaponifiables de soja et d'avocat adminstrés par voie percutanée chez le rat sans poils
Microbicides to Prevent HIV Transmission: Overcoming Obstacles to Chemical Barrier Protection (2005)

Safe and effective topical microbicides are biologically plausible. Several trials that are under way may demonstrate the ability of microbicides to protect against transmission of HIV, but multiple challenges remain.
Hish speed deep packet inspection method with low power dissipation (2011)

Hish speed deep packet inspection using TCAM faces the problem of high power dissipation.A 2-phase DPI method BF-TCAM was proposed.The first phase used parallel Bloom filters to exclude the normal packets not including attack signatures.The second phase used TCAM to inspect suspicious packets including real attack packets and false positive packets of the first phase.The Bloom filters' false negative probability is zero and false positive probability is very low.Since most of the network data traffic does not include attack signatures,the method can get high speed deep packet inspection with low power dissipation.
Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae (2003)

Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member of the Caulimoviridae family. All characteristic domains conserved in plant pararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.
Information Extraction and Machine Learning: Auto-Marking Short Free Text Responses to Science Questions (2005)

Traditionally, automatic marking has been restricted to item types such as multiple choice that narrowly constrain how students may respond. More open ended items have generally been considered unsuitable for machine marking because of the difficulty of coping with the myriad ways in which credit-worthy answers may be expressed. Successful automatic marking of free text answers would seem to presuppose an advanced level of performance in automated natural language understanding. However, recent advances in computational linguistics techniques have opened up the possi-bility of being able to automate the marking of free text responses typed into a computer without having to create systems that fully understand the answers. This paper describes the use of information extraction and machine learning techniques in the marking of short, free text responses of up to around five lines.
A new method for constructing tumor specific gene co-expression networks based on samples with tumor purity heterogeneity (2018)

Supplementary data are available at Bioinformatics online.
Murine C4b-binding protein. Mapping of the ligand binding site and the N-terminus of the pre-protein (1993)

Abstract We have constructed cell surface-bound forms of murine C4b-binding protein (mC4BP) that allowed us to monitor the binding of mC4BP to C4b with relatively simple erythrocyte rosette assays. We used two types of surface-bound mC4BP: one in which segments of mC4BP were fused directly to a peptide containing the transmembrane and cytoplasmic domains of human complement receptor CR2 (BPR1-type); and a second in which the same segments were fused to a longer peptide containing the five membrane-proximal short consensus repeats (SCR) of CR2 as well as the transmembrane and cytoplasmic domains (BPR2-type). COS cells transfected with either construct carrying all six mC4BP SCR rosetted with C4b-bearing EAC14 cells but not with C4b-lacking EAC1 cells; and rosetting was inhibited by excess inactivated C4 but not inactivated C3. COS cells transfected with BPR2 constructs carrying only SCR 1-3 or 1-4 gave similar rosetting behavior. However, rosetting was not observed with BPR2 constructs carrying only SCR 1-2 or 2-6, or with BPR1 constructs carrying only SCR 1-2, 1-3, 1-4, or 2-6. Finally, we found that alteration of the AUG sequence 56 triplet codons upstream of the putative N-terminus of mature mC4-BP eliminates rosetting whereas alteration of a second AUG sequence 13 codons upstream has no effect on rosetting. These results indicate that 1) SCR 1-3 of mC4BP are necessary and adequate for binding to C4b, 2) steric effects close to the cell surface may interfere with binding, and 3) mC4BP has an extraordinarily long 56 amino acid residue signal peptide.
The N-terminal region of ABC50 interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2 (2007)

ABC50 is an ABC (ATP-binding cassette) protein which, unlike most ABC proteins, lacks membrane-spanning domains. ABC50 interacts with eIF2 (eukaryotic initiation factor 2), a protein that plays a key role in translation initiation and in its control, and in regulation of ribosomes. Here, we establish that the interaction of ABC50 with eIF2 involves features in the N-terminal domain of ABC50, the region of ABC50 that differs most markedly from other ABC proteins. This region also shows no apparent similarity to the eIF2-binding domains of other partners of eIF2. In contrast, the N-terminus of ABC50 cannot bind to ribosomes by itself, but it can in conjunction with one of the nucleotide-binding domains. We demonstrate that ABC50 is a phosphoprotein and is phosphorylated at two sites by CK2. These sites, Ser-109 and Ser-140, lie in the N-terminal part of ABC50 but are not required for the binding of ABC50 to eIF2. Expression of a mutant of ABC50 in which both sites are mutated to alanine markedly decreased the association of eIF2 with 80S ribosomal and polysomal fractions.
Physical Conditioning Facilitates the Exercise-Induced Secretion of Beta-Endorphin and Beta-Lipotropin in Women (1981)

EXERCISE training is used increasingly to prevent and treat disease, and millions of healthy persons participate in strenuous sports; yet, the mechanisms by which exercise produces various clinical effects are imperfectly understood. Emerging evidence suggests that endogenous opioid peptides influence diverse functions linked with the body's energy balance, including appetite,1 thermorégulation,2 lipolysis,3 and reproduction.4 Preliminary observations of athletes indicate that the acute expenditure of energy during exercise stimulates secretion of opioid peptides, but the lack of data on endocrine functions in subjects of experiments and the wide range of reported basal concentrations of hormones weaken this conclusion.5 6 7 No studies have . . .
Backscattered electron imaging to visualize arterial endothelial detachment in the scanning electron microscope. (1983)

The endothelium of the central ear artery of an anesthetized rabbit was damaged by placing artery forceps on the ear over the vessel for 30 min. After removal of the forceps, blood was allowed to flow through the injured vessel for 30 min. Blood flow was then stopped, blood was washed out of the artery in situ and initial fixation with 1% glutaraldehyde was begun. Specimens of the vessel containing the damaged region were postfixed in 3% cacodylate buffered glutaraldehyde and prepared for SEM examination after methenamine silver staining or following post fixation with osmium. Backscattered electron imaging (BEI) of silver stained specimens with inverted signal polarity showed "black" endothelial nuclei similar to darkly stained nuclei of conventional light microscope slide preparations. Secondary electron imaging (SEI) of corresponding sites stained with silver also showed endothelial nuclei. An interruption of the normal pattern of nuclei indicated a detached area of endothelium where subendothelium was exposed. Platelets were observed adhering to the surface of the exposed subendothelium. Injury to the endothelium was seen more readily in silver stained preparations as voids in the nuclear pattern as compared with conventional SEI preparations, postfixed with osmium, which did not show well defined nuclei.
A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli (1997)

A chromosomal DNA fragment from the erythromycin-sensitive bacterium Corynebacterium glutamicum ATCC 13032 was shown to mediate resistance against erythromycin, tetracycline, puromycin, and bleomycin in Escherichia coli. Multicopy cloning of the fragment did not cause a resistance phenotype in C. glutamicum. The corresponding gene encodes a hydrophobic protein with 12 potential transmembrane-spanning ex-helical segments showing similarity to drug-H+ antiporters.
Patient safety - the role of human factors and systems engineering. (2010)

Patient safety is a global challenge that requires knowledge and skills in multiple areas, including human factors and systems engineering. In this chapter, numerous conceptual approaches and methods for analyzing, preventing and mitigating medical errors are described. Given the complexity of healthcare work systems and processes, we emphasize the need for increasing partnerships between the health sciences and human factors and systems engineering to improve patient safety. Those partnerships will be able to develop and implement the system redesigns that are necessary to improve healthcare work systems and processes for patient safety.
Synthesis of Indoleacetic Acid from Glutamic Acid and a Proposed Mechanism for the Conversion<sup>1</sup> (1951)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Indoleacetic Acid from Glutamic Acid and a Proposed Mechanism for the Conversion1Sidney W. Fox and Milon W. BullockCite this: J. Am. Chem. Soc. 1951, 73, 6, 2754–2755Publication Date (Print):June 1, 1951Publication History Published online1 May 2002Published inissue 1 June 1951https://pubs.acs.org/doi/10.1021/ja01150a094https://doi.org/10.1021/ja01150a094research-articleACS PublicationsRequest reuse permissionsArticle Views270Altmetric-Citations34LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Population pharmacokinetics and pharmacodynamics of enoxaparin in unstable angina and non‐ST‐segment elevation myocardial infarction (2003)

Enoxaparin clearance depends on body weight, and, therefore, weight-adjusted dosing is recommended to minimize interpatient variability in drug exposure and the risk of haemorrhage. The importance of an increased risk of haemorrhage with decreasing renal function must be weighed against the benefit of treatment with enoxaparin in patients with UA and NSTEMI.
PLATE FIXATION FOR OPEN MANDIBULAR FRACTURES (1985)

Eighty-one patients with mandibular fractures were treated between November 1978 and November 1983. Twenty-six of these patients had 47 comminuted open fractures treated with rigid internal fixation utilizing AO compression plating. No infected nonunion, osteomyelitis, or plate extrusion occurred. Occlusal relationships of remaining dentition were maintained with normal mandibular function in all patients. Rigid internal fixation is an excellent method for stabilizing and repairing comminuted open mandibular fractures. The technique provides immediate return of mandibular function which may be critical in the management of the polytraumatized patient.
DESIGN AND PERFORMANCE OF A TACTILE SHAPE DISPLAY USING RC SERVOMOTORS (2003)

Tactile displays are used to convey small-scale force and shape information to the fingertip. We describe a 6 x 6 tactile shape display design that is low in cost and easily constructed. It uses commercially available RC servomotors to actuate an array of mechanical pins. The pins deflect a maximum of 2 mm, with a resolution of 0.1 mm. The pin center spacing is 2 mm and the pin diameter is 1 mm. For the maximum deflection of 2 mm, the display can represent frequencies up to 7.5 Hz; smaller deflections lead to achievable frequencies up to 25 Hz because the servos are slew rate limited. This design is well suited to tactile display research, as it offers reasonable performance in a robust and inexpensive package.
Assessment of boar sperm samples by computer-assisted sperm anaylsis and the mobility assay (2007)

The sperm mobility assay (Accudenz) used in the present study measures sperm penetration into a biologically inert gradient solution. When a sample of sperm is overlaid in a cuvette containing Accudenz, sperm penetrate the solution and then measured with a spectrophotometer. The mobility assay has been successfully used to select chicken and turkey sperm donors. We validated this assay for semen from boars. Absorbance was measured after overlaying stored semen (24h) from each boar in prefilled cuvettes for 1, 5, 10, 15, 20, 40 min. There were no significant differences between sperm concentrations 1 x 108 and 5 x 107 viable sperm/mL. Absorbance was half-maximal at 13.1 min. In addition, there was medium repeatability for individual boars. There were positive correlations between mobility values and several computer-assisted sperm analysis (CASA) measurements, and low to high reproducibility for several CASA measurements. We concluded that further study is needed to find the proper Km and, the mobility and CASA measurements are correlated.