gebhart/patrepeval huggingface dataset:
Secrets of Soil Survival Revealed by the Genome Sequence of Arthrobacter aurescens TC1 (2006)

Arthrobacter sp. strains are among the most frequently isolated, indigenous, aerobic bacterial genera found in soils. Member of the genus are metabolically and ecologically diverse and have the ability to survive in environmentally harsh conditions for extended periods of time. The genome of Arthrobacter aurescens strain TC1, which was originally isolated from soil at an atrazine spill site, is composed of a single 4,597,686 basepair (bp) circular chromosome and two circular plasmids, pTC1 and pTC2, which are 408,237 bp and 300,725 bp, respectively. Over 66% of the 4,702 open reading frames (ORFs) present in the TC1 genome could be assigned a putative function, and 13.2% (623 genes) appear to be unique to this bacterium, suggesting niche specialization. The genome of TC1 is most similar to that of Tropheryma, Leifsonia, Streptomyces, and Corynebacterium glutamicum, and analyses suggest that A. aurescens TC1 has expanded its metabolic abilities by relying on the duplication of catabolic genes and by funneling metabolic intermediates generated by plasmid-borne genes to chromosomally encoded pathways. The data presented here suggest that Arthrobacter's environmental prevalence may be due to its ability to survive under stressful conditions induced by starvation, ionizing radiation, oxygen radicals, and toxic chemicals.
LAW AND CONSCIENCE (1937)

The age in which we live presents a rapidly growing cleavage between law and conscience; and this cleavage is resulting inevitably in the destruction of both. It is due almost entirely to the subjectivism which has been steadily gaining ground for the last four hundred years. Once private judgment is admitted as a principle in the conduct of human, affairs the objective norms of human action cease to function as such and both conscience and law are deprived of their vitality. As early as the sixteenth century an incipient subjectivism made its appearance in Philosophy as well as in Religion; it is possible, indeed, that the effort to segregate Philosophy from Religion was the source of the whole evil. For when Religion has ceased to provide a philosophy of life it is no longer true religion, and its sanctions lose their compelling force. Moreover, Philosophy unallied to Religion is as a ship without rudder; it follows the caprice of any intellectual wind that blows. The purpose and the very content of human nature is lost sight of; law becomes an unstable shadow of its real self; and conscience, left to stand alone, is no more than a blind guide leading the blind. This cleavage was not suddenly wrought; nor has it come about without protest; Thomas More, whom we now rejoice to call Saint, was amongst the first to protest against it and thought it worth while to seal his protest with his blood.
Highly Stable Glassy Carbon Interfaces for Long-Term Neural Stimulation and Low-Noise Recording of Brain Activity (2017)

We report on the superior electrochemical properties, in-vivo performance and long term stability under electrical stimulation of a new electrode material fabricated from lithographically patterned glassy carbon. For a direct comparison with conventional metal electrodes, similar ultra-flexible, micro-electrocorticography (μ-ECoG) arrays with platinum (Pt) or glassy carbon (GC) electrodes were manufactured. The GC microelectrodes have more than 70% wider electrochemical window and 70% higher CTC (charge transfer capacity) than Pt microelectrodes of similar geometry. Moreover, we demonstrate that the GC microelectrodes can withstand at least 5 million pulses at 0.45 mC/cm<sup>2</sup> charge density with less than 7.5% impedance change, while the Pt microelectrodes delaminated after 1 million pulses. Additionally, poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT-PSS) was selectively electrodeposited on both sets of devices to specifically reduce their impedances for smaller diameters (<60 μm). We observed that PEDOT-PSS adhered significantly better to GC than Pt, and allowed drastic reduction of electrode size while maintaining same amount of delivered current. The electrode arrays biocompatibility was demonstrated through in-vitro cell viability experiments, while acute in vivo characterization was performed in rats and showed that GC microelectrode arrays recorded somatosensory evoked potentials (SEP) with an almost twice SNR (signal-to-noise ratio) when compared to the Pt ones.
Intermolecular Interactions in .alpha. Helical Oligo- and Poly(L-glutamic acid) at Acidic pH (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntermolecular Interactions in .alpha. Helical Oligo- and Poly(L-glutamic acid) at Acidic pHErik J. Spek, Youxiang Gong, and Neville R. KallenbachCite this: J. Am. Chem. Soc. 1995, 117, 43, 10773–10774Publication Date (Print):November 1, 1995Publication History Published online1 May 2002Published inissue 1 November 1995https://pubs.acs.org/doi/10.1021/ja00148a032https://doi.org/10.1021/ja00148a032research-articleACS PublicationsRequest reuse permissionsArticle Views229Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Systematic development of design of experiments (DoE) optimised self-microemulsifying drug delivery system of Zotepine (2017)

The aim of present investigation is to improve dissolution rate of poor soluble drug Zotepine by a self-microemulsifying drug delivery system (SMEDDS). Ternary phase diagram with oil (Oleic acid), surfactant (Tween 80) and co-surfactant (PEG 400) at apex were used to identify the efficient self-microemulsifying region. Box-Behnken design was implemented to study the influence of independent variables. Principal Component Analysis was used for scrutinising critical variables. The liquid SMEDDS were characterised for macroscopic evaluation, % Transmission, emulsification time and in vitro drug release studies. Optimised formulation OL1 was converted in to S-SMEDDS by using Aerosil<sup>®</sup> 200 as an adsorbent in the ratio of 3:1. The S-SMEDDS was characterised by SEM, DSC, globule size (152.1 nm), zeta-potential (-28.1 mV), % transmission study (98.75%), in vitro release (86.57%) at 30 min. The optimised solid SMEDDS formulation showed faster drug release properties as compared to conventional tablet of Zotepine.
Diabetes-prone and diabetes-resistant BB rats share a common major diabetes susceptibility locus, iddm4: additional evidence for a "universal autoimmunity locus" on rat chromosome 4. (1999)

Diabetes-prone (DP) BB rats develop autoimmune type 1 diabetes spontaneously. At least five loci are linked to disease expression: the major histocompatibility complex (iddm2), two susceptibility loci (iddm4, iddm5), and, possibly, a resistance locus (iddm3). Spontaneous disease also requires homozygosity for lyp/iddm1, which causes lymphopenia. It has not been determined whether lyp/iddm1 is required for predisposition to diabetes autoimmunity in addition to being required for its spontaneous expression. We analyzed backcross rats segregating for diabetes but not lymphopenia using Wistar-Furth (WF) and diabetes-resistant (DR) BB animals. The latter are nonlymphopenic (lyp+/+) and develop diabetes only in response to immunological perturbants. Treatment of (DR-BB x WF)F1 x WF animals (all lyp+/+) using a standard induction protocol caused type 1 diabetes in 58% of progeny. Expression of type 1 diabetes was strongly linked to iddm4. The results suggest that lyp/iddm1 does not determine the predisposition to autoimmunity in BB rats and that iddm4 is a major diabetogenic locus in both DP- and DR-BB animals. The iddm4 gene maps to a region containing several major autoimmunity loci, including aia2, aia3, and cia3. We propose that BB rat diabetes requires 1) class II RT1u (iddm2) for susceptibility, 2) additional loci for disease initiation and progression in response to perturbants, and 3) lyp for spontaneous disease.
Release of histamine from human leukocytes stimulated with the tumor-promoting phorbol diesters. I. Characterization of the response. (1981)

Abstract The tumor promoter and irritant, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) has been found to release histamine from human leukocyte preparations. TPA (100 ng/ml) released from 40 to 80% of the total histamine from the cells of 14 different donors during a 45-min incubation. Other phorbol diesters released histamine but were less potent than TPA (TPA &gt; PDD &gt; PDBz &gt; PDA &gt; mTPA). The histamine-releasing activity of the phorbol diesters correlated very closely not only with binding to a specific receptor in chicken embryo fibroblasts (r = 0.985), but also with their biologic activity in other systems. The rate of TPA-induced histamine release was slower than that of anti-lgE, whereas the temperature dependence was similar (optimum release 37°C, 50% decrease at 30°C, no release below 22°C or after a 30-min preincubation at 47°C). TPA caused histamine release in the absence of extracellular calcium, although the addition of calcium to the medium augmented the release. Release was not inhibited by 3 mM dibutyryl cyclic AMP, which did inhibit anti-lgE-induced release. Both TPA- and anti-lgE-induced release were inhibited by 2-deoxyglucose. Three known inhibitors of arachidonic acid metabolism, phenidone, 5,8,11,14-ei-cosatetraynoic acid, and 5,8,11-eicosatriynoic acid inhibited TPA-induced histamine release. The phospholipase inhibitor, bromphenacyl bromide, was also an effective inhibitor of TPA-induced histamine release. These findings suggest that the phorbol diesters are a unique class of histamine-releasing compounds that share with IgE-mediated stimuli some pathways in lipid metabolism but that are not regulated by cyclic AMP. The identification by others of a phorbol diester receptor, and the findings here that phorbol diesters cause histamine release suggest that the release of mediators important in allergic disease may occur by as yet undiscovered mechanisms operative in vivo.
How to isolate cardiac myocytes (1994)

A complete technique is described for the isolation of myocytes from mammalian hearts using the Langendorff perfusion technique. The use of calcium-free solution containing collagenase and protease, followed by low calcium solution, consistently results in a large number of calcium tolerant myocytes which are well suited for long periods of electrophysiological recording.
True (2000)

AQ4 (1,4-Bis-[[2-(dimethylamino-N-oxide)ethyl]amino]5,8-dihydroxyanthrace ne-9, 10-dione) is a prodrug designed to be excluded from cell nuclei until bioreduced in hypoxic cells to AQ4, a DNA intercalator and topoisomerase II poison. Thus, AQ4N is a highly selective bioreductive drug that is activated in, and is preferentially toxic to, hypoxic cells in tumours. Five murine tumours (MAC16, MAC26, NT, SCCVII and RIF-1) have been used to investigate the anti-tumour effects of AQ4N. In only one tumour (MAC16) was AQ4N shown to be active as a single agent. However, when combined with methods to increase the hypoxic tumour fraction in RIF-1 (by physical clamping) and MAC26 tumours (using hydralazine) there was a substantial enhancement in anti-tumour effect. Notably, RIF-1 tumours treated with AQ4N (250 mg kg(-1)) followed 15 min later by physically occluding the blood supply to the tumour for 90 min, resulted in a 13-fold increase in growth delay. When combined with radiation or chemotherapy, AQ4N substantially increased the effectiveness of these modalities in a range of in vivo model systems. AQ4N potentiates the action of radiation in both a drug and radiation dose-dependent manner. Further the enhancement observed is schedule-independent with AQ4N giving similar effects when given at any time within 16 h before or after the radiation treatment. In combination with chemotherapy it is shown that AQ4N potentiates the activity of cyclophosphamide, cisplatin and thiotepa. Both the chemotherapeutic drugs and AQ4N are given at doses which individually are close to their estimated maximum tolerated dose (data not included) which provides indirect evidence that in the combination chemotherapy experiments there is some tumour selectivity in the enhanced action of the drugs.
Manipulator Calibration Using a Single Endpoint Contact Constraint (2000)

Abstract Most manipulator calibration techniques require expensive and/or complicated pose measuring devices, such as theodolites. This paper investigates a calibration method where the manipulator endpoint is constrained to a single contact point and executes self-motions. From the easily measured joint angle readings, and an identification model, the manipulator is calibrated. Adding a wrist force sensor allows for the calibration of elastic effects due to end-point forces and moments. Optimization of the procedure is discussed. Experimental results are presented, showing the effectiveness of the method.
Micro Ratcheting Transmission Systems Based on Electrostatic Actuator (2008)

This paper presents design and fabrication of a silicon micro ratcheting transmission system (RTS) that utilizes electrostatic comb-drive actuators to rotate a gear ring (or rotor) through a ratchet mechanism. The rotational actuator is engaged with the gear ring through ratchet teeth at one end, and reciprocally rotates around an elastic point at the other end based on electrostatic force. Rotational motion and torque from driving gear ring are transmitted smoothly to driven gears through involute profile gear teeth. Novelty design of anti-gap structures have overcome the unavoidable gap problem occurred in deep reactive ion etching of silicon. The micro RTS has been fabricated and tested successfully. The rotation of the motor is linearly proportional to driving frequency up to 40 Hz.
ATP-induced arachidonic acid release in cultured astrocytes is mediated by Gi protein coupled P2Y1 and P2Y2 receptors (1998)

ATP-induced arachidonic acid (AA) release was studied in [3H]AA-prelabeled cultured astrocytes. To characterize the P2 purinoceptor-mediated effect of ATP, the subtype-specific agonists 2-methylthio ATP (2-MeSATP) and UTP were compared. ATP, UTP, or 2-MeSATP induced a dose-dependent increase of [3H]AA release, with EC50 values of 22.7 microM, 29.4 microM, and 1.68 microM, respectively; alpha,beta-methyleneATP and adenosine had no effect. The order of potency was ATP = UTP > or = 2-MeSATP, indicating that ATP interacted with both P2Y1 and P2Y2 receptors to mediate AA release in astrocytes. The effect of ATP, UTP, or 2-MeSATP was markedly inhibited by pretreatment of cells with pertussis toxin. Ca2+ ionophore-A23187 and PKC activator-TPA mimicked the effects of these three agonists to stimulate AA release. ATP, UTP, and 2-MeSATP induced a rapidly initial rise of [Ca2+]i and a sustained [Ca2+]i increase. The AA release was blocked in the external Ca2+ free in condition the sustained [Ca2+]i increase was abolished. Both A23187- and TPA-induced AA release were also blocked in this condition. Furthermore, inorganic Ca2+ channel blocker Co2+ inhibited ATP, UTP, or 2-MeSATP induced AA release as well. Long-term (24 h) treatment of cells with TPA resulted in an attenuation of three agonists, TPA or A23187 response. Similarly, ATP or TPA promoted AA release was inhibited by the mitogen-activated protein kinase (MAPK) cascade inhibitor PD 98059. ATP, TPA, or A23187 induced an increase in the activity and tyrosine phosphorylation of p42 MAPK, as well as a molecular weight shift, consistent with phosphorylation, of cytosolic phospholipase A2 (cPLA2). ATP- and TPA-stimulated activation of p42 MAPK activity and tyrosine phosphorylation were inhibited by long-term TPA treatment, while A23187-stimulated effects were completely blocked. Furthermore, tyrosine phosphorylation and activation of p42 MAPK and mobility shift of cPLA2 induced by A23187 were reversed in the absence of external Ca2+, suggesting the involvement of PKCalpha in MAPK activation and mobility shift of cPLA2. Taken together, ATP-stimulated AA release was secondary to the activation of P2Y1 and P2Y2 receptors/PLC pathway. Ca2+ and PKC interact to regulate this response. Elevation of intracellular Ca2+, the mechanism involving extracellular Ca2+ influx, might act partly through PKCalpha activation and in turn MAPK might be activated, leading to cPLA2 phosphorylation and AA release.
Antioxidant Effects of Quinoline Alkaloids and 2,4-Di-tert-butylphenol Isolated from Scolopendra subspinipes (2006)

The oxidized low-density lipoprotein (ox-LDL) plays a critical role at the early stages of atherosclerosis. Thus, the prevention of LDL-oxidation by antioxidants may arrest the progression of atherosclerosis. Two quinoline alkaloids, 3,8-dihydroxyquinoline (1) and 2,8-dihydroxy-3,4-dimethoxyquinoline (3), and 2,4-di-tert-butylphenol (2) were isolated from the dried body of Scolopendra subspinipes. Compounds 1-3 exhibited antioxidant activities on copper-mediated (1: IC50=2.6 microM, 2: IC50=8.2 microM, 3: IC50=63.0 microM), AAPH-mediated oxidation (1: IC50=3.9 microM, 2: IC50=9.9 microM, 3: IC50=71.8 microM), and SIN-1-mediated oxidation (1: 70%, 2: 52%, 3: 29% at 5.0 microM) in the TBARS assay. The antioxidant activities of compounds 1-3 were tested with respect to other parameters, such as the lag time of conjugated diene fromation, relative electrophoretic mobility (REM) of ox-LDL, and apoB-100 fragmentation on copper-mediated LDL-oxidation. In addition, compounds 1-3 showed 1,1-diphenyl-2-picrylhydrasyl (DPPH) radical scavenging activity and compound 1 also exhibited metal chelating activity.
Ethyl rhamnolipids as a renewable source to produce biopolyurethanes (2014)

Responding to increasing environmental concerns, lipids are attractive feedstock to produce bio‐based polyurethanes. In this study, ethyl rhamnolipids were used to synthesize polyurethanes by reacting with 1,6‐hexane diisocyanate (HDI). Rhamnolipids were produced by microbial fermentation, providing a novel source for biopolyurethanes. FTIR and gel content analyses confirmed that the hydroxyl groups on rhamnose residues were reacted with isocyanates. DSC analysis revealed an adjustable glass transition temperature (Tg) from 19 to 50°C. Ethyl rhamnolipids are liquid at 50°C, presumably because rhamnose is connected with fatty acid at a mid‐chain position and the unique structure decreases the tendency to crystallize. The liquid polyols were found to have good mixing and reaction properties. The study may inspire future synthesis of novel lipid‐based polyols. Practical applications: Use of vegetable oil‐based polyols for polyurethane production had been extensively investigated. However, synthesis of these polyols mostly involved introduction of petroleum‐based chemicals such as methanol and 1,2‐propanediol. Rhamnolipids are natural glycolipids. Using ethyl rhamnolipids to prepare polyurethanes provides an alternative, more renewable route. In addition, it inspires synthesis of vegetable oil polyols by introducing natural sugars. Bio‐based polyurethanes were prepared from ethyl rhamnolipids, by reaction with 1,6‐hexane diisocyanate (HDI). Rhamnolipids, obtained from fermentation, are an alternative renewable source of reactants for polyurethane production. The unique structure of rhamnolipids may also inspire future synthesis of novel lipid‐based polyols.
Influence of a holistic color interval on color harmony (2000)

This study investigates how a holistic color interval, i.e., the nondirectional color difference between a pair of colors in a CIELAB uniform color space, influences perceived color harmony. A set of 1035 test color pairs displayed on a CRT was evaluated for the degree of harmony. These test color pairs consist of pairs combined from among the selected 46 test colors evenly distributed in color space. The subjects were asked to select their three preferred colors from these 46 test colors and then to evaluate the degree of harmony of the test color combinations. The color intervals (ΔE) of each test color combination were calculated and treated as values of an independent variable. In addition, the evaluated degrees of color harmony were considered as values of a dependent variable, in which statistical analysis confirmed the relationship: the degree of harmony is a cubic function of the color interval. Moreover, the plot of this relationship allowed us to identify four color intervals: roughly corresponding to the regions of first ambiguity, similarity, second ambiguity, and contrast in Moon and Spencer's model. However, our results indicated that Moon and Spencer's principles for classifying harmonious/disharmonious regions in terms of the color interval for three color attributes—lightness, chroma and hue—may be inappropriate in predicting perceived color harmony. As for the color intervals between a pair of colors considered as a function of the three attributes, the interval for lightness may have a predominant effect on color harmony, expressed in terms of a cubic relationship. Results of the study further demonstrated that the subject's choice of colors significantly influences perceived color harmony. © 2001 John Wiley & Sons, Inc. Col Res Appl, 26, 29–39, 2001