gebhart/patrepeval huggingface dataset:
Oblique incidence and observation electronic speckle-pattern interferometry (1994)

In this paper we discuss an oblique incidence and observation electronic speckle-pattern interferometer, in which we use an anamorphic prism in front of the object. A collimated beam traveling through the prism is partly reflected at the base of the prism. The reflected light is the reference beam and the transmitted light illuminates the diffuse object, thereby generating the object beam. In this scheme the object and the reference beams are collinear. A new scheme that uses two prisms and permits phase stepping is also presented.
The Energetic Basis Underpinning T-cell Receptor Recognition of a Super-bulged Peptide Bound to a Major Histocompatibility Complex Class I Molecule (2012)

Although the major histocompatibility complex class I (MHC-I) molecules typically bind short peptide (p) fragments (8-10 amino acids in length), longer, "bulged" peptides are often be presented by MHC-I. Such bulged pMHC-I complexes represent challenges for T-cell receptor (TCR) ligation, although the general principles underscoring the interaction between TCRs and bulged pMHC-I complexes are unclear. To address this, we have explored the energetic basis of how an immunodominant TCR (termed SB27) binds to a 13-amino acid viral peptide (LPEPLPQGQLTAY) complexed to human leukocyte antigen (HLA) B*3508. Using the crystal structure of the SB27 TCR-HLA B*3508(LPEP) complex as a guide, we undertook a comprehensive alanine-scanning mutagenesis approach at the TCR-pMHC-I interface and examined the effect of the mutations by biophysical (affinity measurements) and cellular approaches (tetramer staining). Although the structural footprint on HLA B*3508 was small, the energetic footprint was even smaller in that only two HLA B*3508 residues were critical for the TCR interaction. Instead, the energetic basis of this TCR-pMHC-I interaction was attributed to peptide-mediated interactions in which the complementarity determining region 3α and germline-encoded complementarity determining region 1β loops of the SB27 TCR played the principal role. Our findings highlight the peptide-centricity of TCR ligation toward a bulged pMHC-I complex.
FILTER BUBBLE (2012)

MedientheoretikerNur wenige haben ein Posting zur Kenntnis
Selective inhibition of prostaglandin biosynthesis by gold salts and phenylbutazone (1975)

Gold salts and phenylbutazone selectively inhibit the synthesis of PGF2alpha and PGE2 respectively. Lowered production of one prostaglandin species is accompanied by an increased production of the other. Selective inhibition by these drugs was observed in the presence of adrenaline, reduced glutathione and copper sulphate under conditions when most anti-inflammatory compounds inhibited PGE2 and PGF2alpha syntheses equally. It is postulated that selective inhibitors may have a different mode of action in vivo and beneficial effects may be related to the endogenous ratio of PGE to PGF required for normal function.
Iridium-Catalyzed, Asymmetric Amination of Allylic Alcohols Activated by Lewis Acids (2007)

The direct, Ir-catalyzed, regio- and enantioselective amination of allylic alcohols with Lewis acid activators to form branched allylic amine products is reported. The reactions of arylamines, benzylic amines, and secondary aliphatic amines in the presence of Nb(OEt)5 as activator occurred with high regioselectivities and high enantioselectivities. These results led to the development of Ir-catalyzed reactions of allylic alcohol with arylamines and BPh3 as activator in catalytic amounts. These reactions are rare examples of enantioselective substitutions of allylic alcohols. They are particularly unusual examples of the substitution of allylic alcohols to generate branched substitution products from monosubstituted allylic alcohols and of enantioselective substitutions of allylic alcohols with amine nucleophiles.
160 Gbit/s all-optical time-division demultiplexing utilising modified multiple-output OTDM demultiplexer (MOXIC) (2002)

The first successful demonstration of error-free simultaneous four-channel-output demultiplexing of 160 Gbit/s OTDM signals is reported. The key is a modification to the authors original multiple-output demultiplexer, MOXIC, that significantly increases the stability of its operation owing to improving on/off extinction ratios of demultiplexed signals.
The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: purification and characterization of the recombinant form (1997)

The recombinant form of the cellulase CelF of Clostridium cellulolyticum, tagged by a C-terminal histine tail, was overproduced in Escherichia coli. The fusion protein was purified by affinity chromatography on a Ni-nitrilotriacetic acid column. The intact form of CelF (Mr, 79,000) was rapidly degraded at the C terminus, giving a shorter stable form, called truncated CelF (Mr, 71,000). Both the entire and the truncated purified forms degraded amorphous cellulose (kcat = 42 and 30 min(-1), respectively) and microcrystalline cellulose (kcat = 13 and 10 min(-1), respectively). The high ratio of soluble reducing ends to insoluble reducing ends released by truncated CelF from amorphous cellulose showed that CelF is a processive enzyme. Nevertheless, the diversity of the cellodextrins released by truncated CelF from phosphoric acid-swollen cellulose at the beginning of the reaction indicated that the enzyme might randomly hydrolyze beta-1,4 bonds. This hypothesis was supported by viscosimetric measurements and by the finding that CelF and the endoglucanase CelA are able to degrade some of the same cellulose sites. CelF was therefore called a processive endocellulase. The results of immunoblotting analysis showed that CelF was associated with the cellulosome of C. cellulolyticum. It was identified as one of the three major components of cellulosomes. The ability of the entire form of CelF to interact with CipC, the cellulosome integrating protein, or mini-CipC1, a recombinant truncated form of CipC, was monitored by interaction Western blotting (immunoblotting) and by binding assays using a BIAcore biosensor-based analytical system.
Interfacial Nanoarchitectonics: Lateral and Vertical, Static and Dynamic (2013)

The exploration of nanostructures and nanomaterials is essential to the development of advanced functions. For such innovations, nanoarchitectonics has been proposed as a novel paradigm of nanotechnology aimed at assembling nanoscale structural units into predesigned configurations or arrangements. In this Feature Article, we provide an overview of several recent research works from the viewpoint of interfacial nanoarchitectonics with features developed in lateral directions or grown in vertical directions with construction on solid, static, or flexible dynamic surfaces. Lateral nanoarchitectonics at a static interface provides molecular organization by bottom-up nanoarchitectonics and can also be used to realize device integration by top-down nanoarchitectonics. In particular, in the latter case, the fabrication of novel devices, so-called atomic switches, are introduced as a demonstration of atomic-level electronics. Lateral nanoarchitectonics at dynamic interfaces is exemplified by 2D molecular patterning and molecular machine operation induced by macroscopic motion. The dynamic nature of interfaces enables us to operate molecular-sized machines by macroscopic mechanical stimuli such as our hand motion, which we refer to as hand-operated nanotechnology. Vertical nanoarchitectonics is mainly discussed in relation to layer-by-layer (LbL) assembly. By using this technique, we can assemble a variety of functional materials in ultrathin film structures of defined thickness and layer sequence. The organization of biomolecules (or even living cells) within thin films and their integration with device structures is exemplified. Finally, the anticipated research directions of interfacial nanoarchitectonics are described.
USE OF ESSENTIAL OIL OF LAVENDER ANGUSTIFOLY TO IMPROVE SLEEP QUALITY (2022)

Aromatherapy is an integrative and complementary practice practiced for centuries, which uses essential oils for therapeutic purposes.These substances are products of volatile concentration from a plant-derived source and can be applied topically, inhaled and orally.The Lavander angustifolia It is used to improve sleep quality for its anxiolytic and sedative properties.Thus, the objective of this work is to verify the use of essential oil of Lavander angustifolia with aromatherapy, in improving sleep quality.A bibliographic review was carried out.The selection of research sources was based on publications found in databases such as PUBMED, Google academic and SciELO.Among the articles found, those dated between 2012 and 2022, and published in Portuguese and English, were selected.Some studies show positive results from the use of lavender essential oil in the quality of sleep due to the simple way of application and no adverse effects.
Helical Wrapping and Insertion of Graphene Nanoribbon to Single-Walled Carbon Nanotube (2011)

Molecular dynamics simulations are performed to demonstrate that the graphene nanoribbon (GNR) could helically wrap onto and insert into the single-walled carbon nanotube (SWNT) to form helical configurations, which are quite close to the helices found in nature. The steady decline of the potential energy suggests that the helical wrapping and insertion are spontaneous. The van der Waals interaction, π–π stacking interaction between the GNR and the SWNT, and dangling σ-orbitals on carbon atoms at open edges of GNR are all responsible for these unique phenomena. Two GNRs would form a DNA-like double-helix with the same handedness. The dependence of the diameter and chirality of SWNT and the width of GNR in the helix-forming process are investigated. The gaps on the tube walls are not good at the helical wrapping and insertion. In addition, we explore the possibility of using the spontaneous wrapping and insertion of GNRs to deliver substances. These unique properties are expected to provide novel strategies to design nanoscale vehicles and substantial functional devices.
Cyclic nucleotide phosphodiesterase isoenzyme activities in human alveolar macrophages (1995)

These results indicate that human monocytes and alveolar macrophages are distinct targets for the effects of selective PDE inhibitors while alveolar macrophages from normal and atopic individuals appear to be equally sensitive.
Genomic rearrangements in theBRCA1 andBRCA2 genes (2005)

Mutations in the BRCA1 and BRCA2 genes predispose women to breast and ovarian cancer. BRCA1 and BRCA2 are 83 and 86 kb long, with coding sequences of 5.7 and 10.2 kb, scattered over 22 and 26 coding exons, respectively. The large majority of the alterations identified in these genes are point mutations and small insertions/deletions. However, an increasing number of large genomic rearrangements are being identified, especially in BRCA1. This review gives a brief overview of the techniques used to screen the BRCA1 and BRCA2 genes for large rearrangements, and describes those for which the breakpoints have been characterized. The principal mechanisms that are thought to lead to their formation, founder effects, and recombination hotspots, are also discussed.
Properties of four composite veneering materials polymerized with different laboratory photo‐curing units (1998)

This study examined properties of four composite veneering materials polymerized with two different photo-curing units for the purpose of evaluating curing performance of the combination of composite material and curing unit. Two microfilled (Dentacolor and Thermoresin LC II) and two hybrid (Cesead II and Solidex) composite materials designed for prosthetic veneer were selected. The respective light sources of the units were a xenon (Dentacolor XS) and a metal halide (Hyper L II) lamp. The composite materials were exposed with the photo-curing unit for 60 s on each side (i.e. from top and bottom). Knoop hardness, compressive strength, flexural strength, flexural modulus, water absorption and water solubility were determined according to standardized testing methods. The specimens exposed with the metal halide unit generally exhibited greater Knoop hardness number, and compressive strength and lower solubility than those exposed with the xenon unit. A microfilled material (Thermoresin LC II) cured with the metal halide unit exhibited significantly improved results for all tests as compared with the same material cured with the xenon unit.
Through Silicon Via(TSV) defect/pinhole self test circuit for 3D-IC (2009)

The next generation of ICs will have greater density and speed than that of their predecessors due to the fact that they can be stacked into 3DICs. A common defect in this emerging technology, however, is that pinholes can form during the process of oxide deposition along the through silicon via (TSV) walls. In this paper, four analog test circuits are explored for the purposes of detecting these pinholes. Each of the circuits uses the leakage current from a single PMOS. By using this leakage current to test the resistance between the TSV and ground, one can determine whether there is a pinhole creating a short between the TSV and the substrate.
Reflection high-energy electron diffraction observation of GaAs surface-prepared ultrasonic running de-ionized water treatment (1991)

GaAs (001) surface ultrasonically cleaned under running de-ionized water (U-RDIW) is investigated by reflection high-energy electron diffraction (RHEED). The RHEED observations of U-RDIW-treated surfaces show a spotty (1×1) pattern at room temperature and a (2×1) streaky surface reconstruction pattern at 360 °C. The experimental results indicate that chemically clean and damage-free GaAs surfaces can be produced by U-RDIW treatment. We discuss surface structures before/after heating using a hydrogen- terminated model.
Quantitative MRI measurement of lung density must account for the change in <i>T</i> with lung inflation (2009)

Data show that T(2) (*) in the lung is very short, and varies considerably with lung volume. Thus, if quantitative assessment of lung density within a breathhold is to be measured accurately, then it is necessary to also determine T(2) (*).
CLASSY: An adaptive maximum likelihood clustering algorithm (1979)

The CLASSY clustering method alternates maximum likelihood iterative techniques for estimating the parameters of a mixture distribution with an adaptive procedure for splitting, combining, and eliminating the resultant components of the mixture. The adaptive procedure is based on maximizing the fit of a mixture of multivariate normal distributions to the observed data using its first through fourth central moments. It generates estimates of the number of multivariate normal components in the mixture as well as the proportion, mean vector, and covariance matrix for each component. The basic mathematical model for CLASSY and the actual operation of the algorithm as currently implemented are described. Results of applying CLASSY to real and simulated LANDSAT data are presented and compared with those generated by the iterative self-organizing clustering system algorithm on the same data sets.
Safety and Clinical Usage of Newcastle Disease Virus in Cancer Therapy (2011)

Newcastle disease virus (NDV) is an avian virus that causes deadly infection to over 250 species of birds, including domestic and wild-type, thus resulting in substantial losses to the poultry industry worldwide. Many reports have demonstrated the oncolytic effect of NDV towards human tumor cells. The interesting aspect of NDV is its ability to selectively replicate in cancer cells. Some of the studies have undergone human clinical trials, and favorable results were obtained. Therefore, NDV strains can be the potential therapeutic agent in cancer therapy. However, investigation on the therapeutic perspectives of NDV, especially human immunological effects, is still ongoing. This paper provides an overview of the current studies on the cytotoxic and anticancer effect of NDV via direct oncolysis effects or immune stimulation. Safety of NDV strains applied for cancer immunotherapy is also discussed in this paper.
Real‐time fluorescence‐based detection of furanocoumarin photoadducts of DNA (2008)

Real-time fluorescence detection systems were adapted to identify DNA adducts formed by photogenotoxic phytochemicals. Two assays were developed: the first was based on quantitative polymerase chain reaction (qPCR) while the second used thermal denaturation and renaturation (D-R). Both assays employed yeast DNA, the fluorescent dye SYBR Green and a real-time PCR thermocycler. The furanocoumarins 8-methoxypsoralen (8-MOP), 5-methoxypsoralen (5-MOP), psoralen, angelicin and imperatorin, and the furanochrome khellin, were tested for adduct forming ability with up to 2 h of UVA light exposure (lambda = 320-400 nm). The known bifunctional compounds, 8-MOP, 5-MOP and psoralen, were inferred to form biadducts here based on both D-R and qPCR assays, as expected from previous research. The known monofunctional compound angelicin was used as a negative control and did not form biadducts based on either assay. Two compounds of unknown functional specificity, imperatorin and khellin, were determined to be positive and negative for biadduct activity, respectively. Detection of biadducts with 8-MOP, 5-MOP, psoralen and imperatorin, but not angelicin or khellin, was further verified by temperature gradient gel electrophoresis. The fluorescence methods improve and expand upon existing assays to monitor DNA adducts.
High-luminance and high-resolution 5-in. polycrystal phosphor screen (2003)

We have developed a 5-in. poly-crystal phosphor screens for projection CRTs using poly-crystal materials as the phosphor screen substrate. The poly-crystal materials has the same excellent physicochemical characteristics as the YAG has such as high thermal conductivity and high intensity as well as high optical transparency, but also the bigger diameter of the poly-crystal faceplate can be easily obtained and the cost of the poly-crystal faceplate is only a tenth of that of the same diameter of YAG. Furthermore, poly-crystal substrate is obtained by stamping technological process. The poly-crystal phosphor screens may be satisfied to the needs of large-scale production. An experimental 48-in. rear-projection TV incorporating the projection tubes achieved more than 1600 TV lines of horizontal resolution and 1800 lumens of brightness.
Long-term reconstitution of the mouse hematopoietic system by embryonic stem cell-derived fetal liver. (1991)

Murine embryonic stem (ES) cells are permanent blastocyst-derived cell lines capable of contributing to a wide variety of tissues, including the germ line, after injection into host blastocysts. Recently, we have shown that ES cells can produce all of the cells of the developing fetus after aggregation with developmentally compromised tetraploid embryos. Completely ES cell-derived embryos die perinatally, but the liver of these embryos is a source of entirely ES cell-derived hematopoietic progenitors. We have taken 14- to 15-day fetal liver cells from ES cell-tetraploid chimeras and reconstituted the hematopoietic system of lethally irradiated adult recipient mice. ES cell-derived hematopoietic stem cells were capable of long-term (greater than 6 months) repopulation of irradiated recipients, and all hematopoietic cell lineages analyzed (erythrocytes, T cells, mast cells, and macrophages) were derived exclusively from ES cells in such recipients. Thus, ES cells retain the capacity to differentiate into all hematopoietic cell types after prolonged passage in culture. This approach should provide a direct route to the production of mice whose hematopoietic cells carry genetic alterations that would be lethal if passed through the germ line.
Genetic Modification of the O-Polysaccharide of Francisella tularensis Results in an Avirulent Live Attenuated Vaccine (2011)

Ft.LVS::Δwzy was avirulent in mice and, despite expressing only 1 repeating unit of the O-PS, induced antibodies to the full-length O-PS. Vaccination with Ft.LVS::Δwzy protected mice against intranasal challenge with both type A and type B strains of F. tularensis and induced functional immunity through both humoral and cellular mechanisms.
Cleansing effect of acidic L-arginine on human oral biofilm (2016)

Dental plaque formed on tooth surfaces is a complex ecosystem composed of diverse oral bacteria and salivary components. Accumulation of dental plaque is a risk factor for dental caries and periodontal diseases. L-arginine has been reported to decrease the risk for dental caries by elevating plaque pH through the activity of arginine deiminase in oral bacteria. Here we evaluated the potential of L-arginine to remove established oral biofilms. Biofilms were formed using human saliva mixed with Brain Heart Infusion broth supplemented with 1 % sucrose in multi-well plates or on plastic discs. After washing the biofilms with saline, citrate (10 mM, pH3.5), or L-arginine (0.5 M, pH3.5), the retained biofilms were analyzed by crystal violet staining, scanning electron microscopy, and Illumina-based 16S rDNA sequencing. Washing with acidic L-arginine detached oral biofilms more efficiently than saline and significantly reduced biofilm mass retained in multi-well plates or on plastic discs. Illumina-based microbiota analysis showed that citrate (pH3.5) preferentially washed out Streptococcus from mature oral biofilm, whereas acidic L-arginine prepared with 10 mM citrate buffer (pH3.5) non-specifically removed microbial components of the oral biofilm. Acidic L-arginine prepared with citrate buffer (pH3.5) effectively destabilized and removed mature oral biofilms. The acidic L-arginine solution described here could be used as an additive that enhances the efficacy of mouth rinses used in oral hygiene.
High-Detail Local Aerial Imaging Using Autonomous Drones (2009)

Aerial Imaging is mainly done with small planes from several kilometres altitude. A small unmanned quadcopter (a type of helicopter with four rotors) is an alternative airborne platform, which allows fast aerial imaging of small areas with a higher level of detail and lower cost. It can be used for updates of GI-Systems after natural phenomena, e.g. update of the tree population of areas affected by windthrow from storms. Another possible application is the detection of tree diseases like rottenness or bug infections. We have developed and performed a prototypic workflow for the update of the “Virtual Forest” 1 , a database and framework for efficient forest planning and wood mobilization, using aerial image data collected with the quadcopter AirRobot ®2 . We have integrated a software component into VEROSIM ® , a software solution for virtual reality and GI-Systems, which allows the autonomous routing, flyover and imaging of a selected geographical unit, e.g. a land parcel, and the subsequent georeferenced display of the aerial images.
Chiral discrimination by modified cyclodextrins (1996)

C. J. Easton and S. F. Lincoln, Chem. Soc. Rev., 1996, 25, 163 DOI: 10.1039/CS9962500163
A high frequency GaN Lamb-wave sensor device (2010)

Lamb-wave devices are characterized by high sensitivity to surface perturbations due to acoustic energy confinement in a thin piezoelectric membrane. In this paper, a Lamb-wave delay line device based on a single crystalline GaN membrane is presented. The observed operating frequency of 178.82 MHz was found to be in excellent agreement with numerical analysis predictions. The device as a chemical sensor was evaluated during the application of various concentrations of glycerol on the device surface; its ability to operate as a biosensor was also assessed during the detection of the specific binding of a protein (IgG) to the device surface.