gebhart/patrepeval huggingface dataset:
An approachable human adult stem cell source for hard‐tissue engineering (2005)

Stem cells were obtained from deciduous dental pulp of healthy subjects, aged 6-10 years. This stem cell population was cultured, expanded, and specifically selected, detecting using a FACsorter, c-kit, CD34, and STRO-1 antigen expression. Then, c-kit+/CD34+/STRO-1+ cells were replaced in the culture medium added of 20% FBS, leading to osteoblast differentiation. In fact, these cells, after a week, showed a large positivity for CD44, osteocalcin, and RUNX-2 markers. To achieve an adipocytic differentiation, cells, after sorting, were challenged with dexamethason 10(-8) mM in the same culture medium. To obtain myotube fusion, sorted cells were co-cultured in ATCC medium with mouse myogenic C2C12 cells and, after a week, human stem cell nuclei were found to be able to fuse, forming myotubes. Differentiated osteoblasts, as assessed by a large positivity to several specific antibodies, after 30 days of culture and already in vitro, started to secrete an extracellular mineralized matrix, which, 2 weeks later, built a considerable number of 3D woven bone samples, which showed a strong positivity to alkaline phosphatase (ALP), alizarin red, calcein, other than to specific antibodies. These bone samples, after in vivo transplantation into immunosuppressed rats, were remodeled in a lamellar bone containing entrapped osteocytes. Therefore, this study provides strong evidence that human deciduous dental pulp is an approachable "niche" of stromal stem cells, and that it is an ideal source of osteoblasts, as well as of mineralized tissue, ready for bone regeneration, transplantation, and tissue-based clinical therapies.
Optimizing method of pancreatic cancer MiaPaCa-2 cells total RNA-transfected dendrtic cells (2011)

Objective To investigate the best method of transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into dendrtic cells (DCs). Methods DCs were cultured from peripheral blood mononuclear cells induced by rhGM-CSF, rhIL-4 and TNF-α. Morphology of DCs was observed. Flow cytometry was used to detect the mature DCs specific surface markers:CD40, HLA-DR, CD83, CD86. Mixed lymphocyte (MLR) was used to determine the ability of DCs to stimulate allogeneic T cell proliferation.Liposomal transfection, electroporation method and passive transfection was used to transfect MiaPaCa-2 cell total RNA into DCs, Real time RT-PCR and MTT assay was used to determine the expression of MUC1 mRNA and the survival rate of the RNA transfected DCs. Results The cells acquired showed typical DCs morphology, the positive rate of CD40, HLA DR, CD83 and CD86 were 34.3% ,50.2% ,89.2% and 73.6%,and they showed a strong ability to stimulate allogeneic T cell proliferation. 48 h after transfection with MiaPaCa-2 cells total RNA by using electroporation, the MUC1 mRNA amount (45.39 ± 9.33) in DCs was higher than that of liposomes method (3 1. 68 ± 7.25) and passive transfection method (18.53 ± 3.26) . DCs survival rate was (80.36 ± 2.43)% by using electroporation, which was relatively lower than (91.48 ±5.42) % by using passive transfection method, but higher than (67.44 ± 2.51) % by using liposomes method,and it was stabilized around 80%. Conclusions Transfecting total RNA extracted from pancreatic cancer MiaPaCa-2 cells into DCs with electroporation is efficient and safe.

Key words: 
Pancreatic neoplasms; Dendritic cells; RNA Transfection
New Instrumentation for Three-Step Follicular Unit Extraction (2006)

1. Robert M. Bernstein, MD 1. New York, New York 2. William R. Rassman, MD 1. Los Angeles, California When we published the technique of follicular unit extraction (FUE) in 2002, Dr. Rassman and I described a 2-step process for this new hair transplant procedure.[1][1] The first step
Pancreatic acinar cells produce, release, and respond to tumor necrosis factor-alpha. Role in regulating cell death and pancreatitis. (1997)

The aim of this study was to determine whether tumor necrosis factor-alpha (TNFalpha) and receptors for TNFalpha are expressed in the exocrine pancreas, and whether pancreatic acinar cells release and respond to TNFalpha. Reverse transcription PCR, immunoprecipitation, and Western blot analysis demonstrated the presence of TNFalpha and 55- and 75-kD TNFalpha receptors in pancreas from control rats, rats with experimental pancreatitis induced by supramaximal doses of cerulein, and in isolated pancreatic acini. Immunohistochemistry showed TNFalpha presence in pancreatic acinar cells. ELISA and bioassay measurements of TNFalpha indicated its release from pancreatic acinar cells during incubation in primary culture. Acinar cells responded to TNFalpha. TNFalpha potentiated NF-kappaB translocation into the nucleus and stimulated apoptosis in isolated acini while not affecting LDH release. In vivo studies demonstrated that neutralization of TNFalpha with an antibody produced a mild improvement in the parameters of cerulein-induced pancreatitis. However, TNFalpha neutralization greatly inhibited apoptosis in a modification of the cerulein model of pancreatitis which is associated with a high percentage of apoptotic cell death. The results indicate that pancreatic acinar cells produce, release, and respond to TNFalpha. This cytokine regulates apoptosis in both isolated pancreatic acini and experimental pancreatitis.
Preparation and Characterization of Agarose Hydrogel Nanoparticles for Protein and Peptide Drug Delivery (1997)

The purpose of this work was to develop and characterize a protein and peptide injectable drug delivery system in agarose hydrogel nanoparticles. The nanoparticles were prepared by using a new emulsion-converted-to-suspension in situ method. This is an emulsifier-free method that has advantages for protein and peptide drug encapsulations. Ovalbumin, used as a model protein drug, was successfully encapsulated into nearly spherical agarose hydrogel nanoparticles under mild conditions. The nanoparticles possessed a log-normal size distribution with an average size of 504 nm. They imbibed a large amount of water (66.85% to 84.33%) and the water content was a function of temperature; the water content increased with increase in temperature. Release studies of the ovalbumin from the agarose hydrogel nanoparticles revealed a diffusion-controlled release mechanism with a temperature dependence; the ovalbumin release rate was higher at 37 degrees C than that at room temperature. The great biocompatibility of agarose hydrogel, plus the mild conditions for drug encapsulation, make the agarose hydrogel nanoparticles a potential system for protein and peptide drug delivery.
A thermal flow sensor with liquid characterization feature (2010)

An enhanced flow sensor is under development at IMTEK to enable simultaneous measurement of thermal properties of a fluid and its flow velocity. AC heating is used to obtain more and detailed information on the fluid properties. Here, we present the flow characteristics of our sensor for liquids and show the influence of thermal conductivity of the fluid on the temperature amplitude at the central thermistor using calibration liquids. We further discuss the influences of fluid thermal properties and flow velocity on the phase of each thermistor signal. Experimental results suggest that phase shift on any thermistor location strongly depends on flow velocity for liquids. They also show that thermal conductivity of liquids can be determined with a minimum accuracy of 85% of their literature values using a linearly interpolated temperature amplitude characteristic of the closest thermistor to the heater.
Formation Energy of Individual Cation Vacancies in LiF and NaCl (1969)

The formation energy g+ of an individual cation vacancy in LiF and NaCl has been measured by means of the temperature dependence of the electrical signals produced by push-pull deformation of bent crystals and application of the theory of charged dislocations. For LiF: g+=0.74±0.04 eV at 680°K. For NaCl: g+=0.75±0.04 eV at 627°K and 0.72±0.04 eV at 761°K. A comparison with other published results for NaCl reveals some unexplained disagreement.
Indolent lymphoma: diagnosis and prognosis in medical practice. (2013)

Although it was a small number of patients, the results obtained correspond to the results existing in literature.
Cholesterol-lowering Action of BNA-based Antisense Oligonucleotides Targeting PCSK9 in Atherogenic Diet-induced Hypercholesterolemic Mice (2012)

Recent findings in molecular biology implicate the involvement of proprotein convertase subtilisin/kexin type 9 (PCSK9) in low-density lipoprotein receptor (LDLR) protein regulation. The cholesterol-lowering potential of anti-PCSK9 antisense oligonucleotides (AONs) modified with bridged nucleic acids (BNA-AONs) including 2',4'-BNA (also called as locked nucleic acid (LNA)) and 2',4'-BNA(NC) chemistries were demonstrated both in vitro and in vivo. An in vitro transfection study revealed that all of the BNA-AONs induce dose-dependent reductions in PCSK9 messenger RNA (mRNA) levels concomitantly with increases in LDLR protein levels. BNA-AONs were administered to atherogenic diet-fed C57BL/6J mice twice weekly for 6 weeks; 2',4'-BNA-AON that targeted murine PCSK9 induced a dose-dependent reduction in hepatic PCSK9 mRNA and LDL cholesterol (LDL-C); the 43% reduction of serum LDL-C was achieved at a dose of 20 mg/kg/injection with only moderate increases in toxicological indicators. In addition, the serum high-density lipoprotein cholesterol (HDL-C) levels increased. These results support antisense inhibition of PCSK9 as a potential therapeutic approach. When compared with 2',4'-BNA-AON, 2',4'-BNA(NC)-AON showed an earlier LDL-C-lowering effect and was more tolerable in mice. Our results validate the optimization of 2',4'-BNA(NC)-based anti-PCSK9 antisense molecules to produce a promising therapeutic agent for the treatment of hypercholesterolemia.
Nuclear criticality safety considerations in the design of high density spent fuel storage racks (1983)

The importance to nuclear criticality safety of various design parameters for poisoned high density spent fuel storage racks is presented and discussed. The most significant design parameters are identified and quantitatively related to criticality requirements over the range of normal interest for storage of both BWR and PWR type fuel assemblies. Criticality considerations of compacted fuel storage are also considered, and the significance of the revision to Reg. Guide 1.13 and of the proposed ANS 8.17 standard on Nuclear Criticality Safety is discussed.
A phase II study of ifosfamide, cisplatin, etoposide in patients with advanced non-small cell lung cancer: a preliminary report. (1990)

Immunocompromised patients are predisposed to opportunistic fungal infections. Candidiasis is reported most frequently both as a localized infection (eg, oropharyngeal candidiasis) and as life-threatening systemic candidiasis. With relatively few antifungal agents in the clinical armamentarium, the optimal management of candidiasis remains controversial. Among the agents that are available, amphotericin B is difficult to administer, 5-fluorocytosine cannot be used alone due to the frequent emergence of resistant yeasts, and ketoconazole, which is effective for esophageal and oropharyngeal candidiasis, is not recommended for systemic candidiasis, especially in granulocytopenic patients. Recently, fluconazole, a new triazole antifungal agent, has been found to be active against Candida spp and is being studied in various clinical settings. In addition to its oral formulation, it is available for intravenous (IV) administration, which is a significant advantage in treating debilitated or noncompliant patients. In a randomized, double-blind study, we compared the efficacy of 100 mg/d oral fluconazole with that of 400 mg/d ketoconazole in cancer patients with oropharyngeal candidiasis. Although clinical and microbiological outcomes were similar for both groups, relapses occurred earlier in ketoconazole- than in fluconazole-treated patients. In another study, we administered fluconazole IV 100 to 300 mg/d to 13 patients, eight of whom had fungemia. Preliminary results are encouraging. Further studies of fluconazole as prophylaxis in granulocytopenic patients and as therapy for documented systemic candidiasis are under way. These studies are expected to define specific indications for fluconazole in immunocompromised patients.
Effects of Cell Lysis on the Rheological Behavior of Red Blood Cell Suspensions (1990)

Rheological studies of lysed cell suspensions are performed with a magneto acoustic ball microrheometer. Two methods for lysing the cells are developed in order to provide cell volume concentrations identical to control intact cell suspensions. The first uses a freeze-thaw technique and the second uses sonication. It is found that cell suspensions disrupted by sonication have a lower viscosity than intact suspensions, whereas cell suspensions lysed by the freeze-thaw method exhibit a higher viscosity. Sonication is discovered to have a detrimental impact on the cell membrane, and to cause complete destruction of the cell membrane structure. Measurements of the steady state viscosity show that indeed the presence of the membrane is not detected, and that what is measured is mainly the viscosity of the hemoglobin solution. On the other hand, freeze-thaw results indicate that at least two phenomena occur. The first phenomenon, occurring during the first freeze-thaw cycle, produces an increase in viscosity and in viscoelasticity. The second one, taking place after subsequent freeze-thaw cycles, induces a decrease in the bulk rheological properties. Several possible mechanisms are presented to explain the observed phenomena.
The Existence and Definition of Philosophy. From an Arabic Text Ascribed to al-Fārābī (1951)

The following pages are translated from an unpublished Bodleian MS. They form the first part of the introduction to an Arabic commentary on the Eisagoge of Porphyry, which the title page and the Bodleian catalogue ascribe to the well-known Arab philosopher al-Fārābī (first half of the tenth century A.D.). We know from independent sources that al-Fārābī wrote a commentary to the Eisagoge. This is evidently what we have before us, but the text dealing with the Eisagoge, which was later itself commented on by Avempace, is a different work.The world empire of the Arabs was already an established fact considerably earlier than A.D. 700, It is generally admitted that the Arabs in the countries which they came to rule were the heirs of classical antiquity to a considerable extent. This is nowhere more evident than in philosophy and the sciences. If at first sight it seems surprising that they made more use of ancient philosophy and science than the general literature and common institutions of the Graeco-Roman world such as the theatre and the gymnasium, the reason is doubtless to be found in the abstract character of these branches, in consequence of which they did not impinge directly on the prejudices of the new rulers.
Architectural Design of Home Energy Saving System Based on Realtime Energy-Awareness (2009)

Recently, a global market related to energy efficiency and energy saving technologies is being extended rapidly with the growth of a matter of primary concern about extreme climate change. Especially, in the home network environments where only its functional aspects have been focused on without considering energy consumption, it is urgent to develop new energy-efficient technologies specialized in the home network environments. In this paper, architecture of home energy saving system (HESS) based on energy-awareness and its basic functional requirements are designed and proposed for real-time home energy monitoring service, reducing or cutting off standby power caused by home appliances, and intelligent home energy management service.
A forum for supporting interactive presentations to distributed audiences (1994)

Computer technology is available to build video-based tools for supporting presentations to distributed audiences, but it is unclear how such an environment affects participants' ability to interact and to learn. We built and tested a tool called Forum that broadcasts live audio, video and slides from a speaker, and enables audiences to interact with the speaker and other audience members in a variety of ways. The challenge was to enable effective interactions while overcoming obstacles introduced by the distributed nature of the environment, the large size of the group, and the asymmetric roles of the participants. Forum was most successful in enabling effective presentations in cases when the topic sparked a great deal of audience participation or when the purpose of the talk was mostly informational and did not require a great deal of interaction. We are exploring ways to enhance Forum to expand the effectiveness of this technology.
Salient stills (2005)

Salient Stills are a class of images that reflect the aggregation of the temporal changes that occur in a moving-image sequence with the salient features of individual frames preserved. They convey the intended expression of an entire series of moving frames---a visual summary of camera and object movements. The original frames, which may include variations in focal length or field of view, or moving objects, are combined to create a single still image. The still image may have multiresolution patches, a larger field of view, or higher overall resolution than any individual frame in the original image sequence. Salient Stills may also contain selected significant objects from individual or multiple video frames. Salient Stills attempt to retain much of the original content (detail) and spatial and temporal extent (context) of the original video or film sequence.
An Overview of Buried Oxides on Silicon: New Processes and Radiation Effects (1996)

This paper presents a review of the main properties of the two types of buried oxides that currently dominate the Silicon-On-Insulator (SOI) technologies: SIMOX (Separation by IMplantation of OXygen) and BESOI (Bond and Etch-Back SOI) materials. After examining the main advantages of SOI structure for radiation-hardened electronics, we present different advanced technological processes of such buried oxides and review their physical characteristics as well as their charge trapping properties under ionizing radiations.
Digital terrain systems (1997)

A digital terrain system (DTS) uses a stored digital map of terrain elevation data together with measurements of the aircraft's dynamics and height above the ground, to provide navigation and terrain referenced cues. The paper provides an overview of DTS, however, as specific designs will differ, the technical information presented is generally based on the BAe TERPROM system. The DTS can provide the following functions: terrain referenced navigation (TRN); terrain following (TF); predictive ground collision avoidance system (PGCAS); obstruction warning and cueing; and ranging. A TRN system is an integrated navigation system, processing and combining data from a dead reckoning system, usually an INS (inertial navigation system), radalt (radar altimeter, gives height above ground level) and a DTED (digital terrain elevation data) map database.
Working abroad (1993)

Nursing jobs abroad are also falling foul of recessionary cutbacks. But there are still jobs to be had. Julia Mountain helps you through the complications
Superfast Transesterification of Triolein Using Dimethyl Ether and a Method for High-Yield Transesterification (2008)

Superfast transesterification of triolein (a biodiesel feedstock model) was achieved in the presence of liquefied dimethyl ether using the co-solvent effect and low viscosity. Furthermore, a method for higher ester yield, in which methanol was additionally introduced into the reaction system immediately before the beginning of phase separation due to byproduct glycerin to maintain the reaction system in a homogeneous state, was proposed. This method led to superfast and high-yield transesterification with a yield exceeding 96% at 3 min.
D-cycloserine in Schizophrenia: New Strategies for Improving Clinical Outcomes by Enhancing Plasticity (2016)

There are preliminary findings that support a role for D-cycloserine in schizophrenia as a strategy to enhance neuroplasticity and memory. However, additional studies with DCS are needed to confirm these findings. In addition, clinical trials with positive and negative allosteric modulators with greater specificity for NMDA receptor subtypes are needed to identify the optimal strategy for enhancing neuroplasticity in schizophrenia.
Real-Time Temporal Maximum-Intensity-Projection Imaging of Hepatic Lesions with Contrast-Enhanced Sonography (2008)

Vascular morphology is depicted at a level or detail not seen before with sonography. High-frame-rate sequences of less than one second uniquely show arterial structure in liver lesions.
Respiratory chain and mitochondrial deoxyribonucleic acid in blood cells from patients with focal and generalized dystonia (1994)

Abstract An increaseing number of neurodegenerative diseases seem to be associated with or even due to disturbancs of cerebral energy metabolism. One generally accepted example is complex I deficiency in substantia nigra from patients with Parkinson's disease. Reports on a complex I defect in platelets from patients with dystonia led us to check for disturbances of the respiratory chain or of the mitochondrial genome in isolated mitochondria from patients with focal or generalized dystonia. We could not confirm the idea of mitochondrial disturbance in platelets from patients with dystonia because we did not find abnormal enzyme activities or any deletions of the mitochondrial genome. Thus, we do not think that blood cells such as platelets can serve as markers for neurodegenerative disorders such as dystonia.
<i>TIF4631</i> and <i>TIF4632</i> : Two Yeast Genes Encoding the High-Molecular-Weight Subunits of the Cap-Binding Protein Complex (eukaryotic initiation factor 4F) Contain an RNA Recognition Motif-Like Sequence and Carry out an Essential Function (1993)

The 5' ends of eukaryotic mRNAs are blocked by a cap structure, m7GpppX (where X is any nucleotide). The interaction of the cap structure with a cap-binding protein complex is required for efficient ribosome binding to the mRNA. In Saccharomyces cerevisiae, the cap-binding protein complex is a heterodimer composed of two subunits with molecular masses of 24 (eIF-4E, CDC33) and 150 (p150) kDa. p150 is presumed to be the yeast homolog of the p220 component of mammalian eIF-4F. In this report, we describe the isolation of yeast gene TIF4631, which encodes p150, and a closely related gene, TIF4632. TIF4631 and TIF4632 are 53% identical overall and 80% identical over a 320-amino-acid stretch in their carboxy-terminal halves. Both proteins contain sequences resembling the RNA recognition motif and auxiliary domains that are characteristic of a large family of RNA-binding proteins. tif4631-disrupted strains exhibited a slow-growth, cold-sensitive phenotype, while disruption of TIF4632 failed to show any phenotype under the conditions assayed. Double gene disruption engendered lethality, suggesting that the two genes are functionally homologous and demonstrating that at least one of them is essential for viability. These data are consistent with a critical role for the high-molecular-weight subunit of putative yeast eIF-4F in translation. Sequence comparison of TIF4631, TIF4632, and the human eIF-4F p220 subunit revealed significant stretches of homology. We have thus cloned two yeast homologs of mammalian p220.