gebhart/patrepeval huggingface dataset:
SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway (1998)

Synaptosomal-associated protein of 25 kDa (SNAP-25) is a palmitoylated membrane protein essential for neurotransmitter release from synaptic terminals. We used neuronal cell lines to study the biosynthesis and posttranslational processing of SNAP-25 to investigate how palmitoylation contributes to the subcellular localization of the protein. SNAP-25 was synthesized as a soluble protein that underwent palmitoylation approximately 20 min after synthesis. Palmitoylation of the protein coincided with its stable membrane association. Treatment of cells with brefeldin A or other disrupters of transport inhibited palmitoylation of newly synthesized SNAP-25 and abolished membrane association. These results demonstrate that the processing of SNAP-25 and its targeting to the plasma membrane depend on an intact transport mechanism along the exocytic pathway. The kinetics of SNAP-25 palmitoylation and membrane association and the sensitivity of these parameters to brefeldin A suggest a novel trafficking pathway for targeting proteins to the plasma membrane. In vitro, SNAP-25 stably associated with membranes was not released from the membrane after chemical deacylation. We propose that palmitoylation of SNAP-25 is required for initial membrane targeting of the protein but that other interactions can maintain membrane association in the absence of fatty acylation.
Membrane Interlayer with Pt Recombination Particles for Reduction of the Anodic Hydrogen Content in PEM Water Electrolysis (2018)

Polymer electrolyte membrane (PEM) water electrolysis is a key technology for sustainable hydrogen based energy supply. Gas permeation through the PEM leads to hydrogen in oxygen at the anode side posing a safety hazard and therefore restricting the operation window of PEM water electrolysis, especially when operating under pressure. In this work the hydrogen in oxygen content at the anode is significantly reduced when a recombination interlayer is integrated into the membrane electrode assemblies (MEAs) compared to reference MEAs without interlayer. The recombination interlayer with a platinum loading of 0.02 mg cm -2 is sprayed between two membranes that are coated with anode and cathode catalysts on the outside. The permeating H 2 and O 2 forms water at the recombination interlayer, leading to higher gas purity and resolving safety issues. In case of the MEAs with interlayer also a constant current hold at 1 A cm -2 for 245 h revealed only a slight increase of the hydrogen in oxygen content (below 14010 -6 vol.% h -1 ) whereas for the reference MEAs without interlayer a stronger increase was observed (above 125010 -6 vol.% h -1 ). Furthermore, the long-term experiments showed no increased degradation rates compared to the reference MEAs.
Abundance, relative gelation activity, and distribution of the 95,000-dalton actin-binding protein from Dictyostelium discoideum. (1983)

We have studied the abundance, relative gelation activity, and distribution of the 95,000-dalton actin-binding protein in Dictyostelium discoideum amoebae. The 95,000-dalton protein was a prominent polypeptide as assessed using quantitative densitometry and radioimmunoassay. We estimated that this protein comprised approximately 1.2% of the protein in a soluble extract of amoebae. The molar ratio of the dimeric 95,000-dalton protein to actin in the soluble extract was 1:30. The apparent viscosities of actin mixtures with either the purified 95,000-dalton protein or the soluble extract were measured by falling ball viscometry in an attempt to assess the contribution of the 95,000-dalton protein to gelation of the soluble extract. The gelation of the soluble extract was significantly less than that expected from the contribution of the 95,000-dalton protein alone. Consequently, we questioned the validity of quantitative analyses of the contributions of specific actin-binding proteins to the gelation of cell extracts. The apparent distribution of the 95,000-dalton protein was observed in chemically fixed and extracted cells by immunofluorescence microscopy and compared with the distribution of cytoplasm and organelles visible using light microscopy. The 95,000-dalton protein was dispersed throughout the cytoplasm of fixed cells, was apparently excluded from prominent organelles, and displayed brightest fluorescence in regions of hyaline cytoplasm. These regions of hyaline cytoplasm that exhibited the brightest fluorescence were observed in the cortical region of rounded cells and in pseudopods of polarized cells. Thus, cell shape and polarity may also have influenced the apparent distribution of the 95,000-dalton protein observed by immunofluorescence microscopy. Study of the distribution of fluorescein-labeled ovalbumin injected into living cells supported the interpretation that the thickness of the cell and the distribution of organelles contributed to the apparent distribution of the 95,000-dalton protein observed in fixed cells using immunofluorescence microscopy. We suggest that the 95,000-dalton protein contributes to modulation of the consistency and contractility of the cytoplasm of D. discoideum amoebae, since it could cross-link actin filaments in vitro in a reversible process that was regulated by changes in the concentration of calcium and of protons, and since it was present in large quantity in the cytoplasm of these cells.
Service Advertisement and Discovery in Mobile Ad hoc Networks (2002)

... component for ad hoc communications and collaboration in ubiquitous computing environments. In this paper, a lightweight service advertisement and discovery protocol for MANET (Mobile Ad hoc NETworks) based on ODMRP (On-Demand Multicast Routing Protocol) is presented. Its service advertisement and discovery information is piggybacked in ODMRP routing control packets. Thus the implementation workload and resource consumption are lightweight as long as mobile devices in MANET support multicast routing functionality. The protocol avoids excessive traffic overhead of periodic advertisements since only updated service advertisements are distributed and the service query/reply mechanism in the pull model is used. Moreover, the pull model deals with mobility in MANET because the advertised and registered service can be obsolete after a certain period of time due to the mobility of either the server or client devices.
A stable, rugged, capacitance-stabilised piezoelectric scanned Fabry-Perot etalon (1981)

In many spectroscopic and interferometric applications, the plate separation of a Fabry-Perot etalon has to be repeatably stepped or scanned over at least one free spectral range. A convenient technique for adjusting the plate separation of an etalon is by means of piezoelectric transducers which may be stabilised by a capacitance micrometer servo-loop. Several optically-contacted etalons have been constructed where the capacitive elements are integral features of the three spacers which also incorporate the piezoelectric transducers. Due to the optical contacting used throughout the device, the etalon is stable against mechanical shock and vibration for most laboratory and field applications. For space-flight applications, however, the spacers have been bonded using cyanoacrylate adhesives.
microRNAs and muscle disorders (2008)

MicroRNAs (miRNAs) are a class of non-coding regulatory RNAs of approximately 22 nucleotides in length. miRNAs are highly conserved across a number of species, including plants, worms and humans. miRNAs regulate gene expression post-transcriptionally, primarily by associating with the 3' untranslated region (UTR) of their regulatory target mRNAs. Recent work has begun to reveal roles for miRNAs in a wide range of biological processes, including cell proliferation, differentiation and apoptosis. miRNAs are expressed in cardiac and skeletal muscle, and dysregulated miRNA expression has been correlated with muscle-related disorders. Genetic studies have identified distinct roles for specific miRNAs during cardiogenesis, cardiac hypertrophy and electrical conduction. Furthermore, conditionally inhibiting the maturation of miRNAs in mouse cardiac and skeletal muscles has revealed that miRNAs are essential for the development and function of those muscles. These previously unrecognized regulators shed new light on the molecular mechanisms that underlie muscle development and pathology, and suggest the potential importance of miRNAs as diagnostic markers and therapeutic targets for muscle-related disease.
Relationships of the Antiproliferative Proteins BTG1 and BTG2 with CAF1, the Human Homolog of a Component of the Yeast CCR4 Transcriptional Complex (2001)

We have reported previously the physical interaction of B-cell translocation gene proteins (BTG)1 and BTG2 with the mouse protein CAF1 (CCR4-associated factor 1) and suggested that these proteins may participate, through their association with CAF1, in transcription regulation. Here we describe the in vitro and in vivo association of these proteins with hPOP2, the human paralog of hCAF1. The physical and functional relationships between the BTG proteins and their partners hCAF1 and hPOP2 were investigated to find out how these interactions affect cellular processes, and in particular transcription regulation. We defined their interaction regions and examined their expression in various human tissues. We also show functional data indicating their involvement in estrogen receptor alpha (ERalpha)-mediated transcription regulation. We found that BTG1 and BTG2, probably through their interaction with CAF1 via a CCR4-like complex, can play both positive or negative roles in regulating the ERalpha function. In addition, our results indicate that two LXXLL motifs, referred to as nuclear receptor boxes, present in both BTG1 and BTG2, are involved in the regulation of ERalpha-mediated activation.
Log Analysis Using Temporal Logic and Reconstruction Approach: Web Server Case (2016)

We present a post-mortem log analysis method based on Temporal Logic (TL), Event Processing Language (EPL), and reconstruction approach. After showing that the proposed method could be adapted to any misuse event or attack, we specifically investigate the case of web server misuses. To this end, we examine 5 different misuses on Wordpress web servers, and generate corresponding log files of these attacks for forensic analysis. Then we establish attack patterns and formalize them by means of a special case of temporal logic, i.e. many sorted first order metric temporal logic (MSFOMTL). Later on, we implement these attack patterns in the EPL, and performed experimental log analysis by using a time window mechanism sliding on sorted log records to evaluate effectiveness and efficacy of our proposed method. We found that our approach is potentially capable of providing a platform where investigators can define/store/share misuse patterns using a common language while providing fast and accurate forensic analysis on large log files.
Development of IR-emitting colloidal II-VI quantum-dot materials (2000)

The current state-of-the-art of colloidal II-VI nanocrystal formation using the aqueous/thiol synthesis route is described. Work on single component and heterostructures and mixed compound quantum dots is discussed. The purpose of the work is to provide a range of infrared (IR)-emitting materials with high quantum efficiency (QE) as potential gain media for future ultrawideband optical amplifiers for high-capacity wavelength-division multiplexing (WDM) telecommunications systems. Physical and chemical factors influencing particle sizes are described.
Autonomous Underwater Vehicles: Trends and Transformations (2005)

Three examples of inter-agency cooperation utilizing current generation, individual Autonomous Underwater Vehicles (AUVs) are described consistent with recent recommendations of the U.S. Commission on Ocean Policy. The first steps in transforming individual AUVs into adaptive, networked systems are underway. To realize an affordable and deployable system, a network-class AUV must be designed with cost–size constraints not necessarily applied in developing solo AUVs. Vehicle types are suggested based on function and ocean operating regime: surface layer, interior and bottom layer. Implications for platform, navigation and control subsystems are explored and practical formulations for autonomy and intelligence are postulated for comparing performance and judging behavior. Laws and conventions governing intelligent maritime navigation are reviewed and an autonomous controller with conventional collision avoidance behavior is described. Network-class cost constraints can be achieved through economies of scale. Productivity and efficiency in AUV manufacturing will increase if constructive competition is maintained. Constructive strategies include interface and operating standards. Professional societies and industry trade groups have a leadership role to play in establishing public, open standards.
Aspirin Use in Patients with Major Upper Gastrointestinal Bleeding and Peptic-Ulcer Disease (1974)

Original Article from The New England Journal of Medicine — Aspirin Use in Patients with Major Upper Gastrointestinal Bleeding and Peptic-Ulcer Disease — A Report from the Boston Collaborative Drug Surveillance Program, Boston University Medical Center
In-Cylinder Air/Fuel Ratio Approximation Using Spark Gap Ionization Sensing (1998)

<div class="htmlview paragraph">Experiments were conducted on a single cylinder engine to measure the ionization current across the spark plug electrodes as a function of key operating parameters including air/fuel ratio. A unique ignition circuit was adapted to measure the ion current as early as 300 microseconds after the initiation of spark discharge.</div> <div class="htmlview paragraph">A strong relationship between air/fuel ratio and features of the measured ion current was observed. This relationship can be exploited via relatively simple algorithms in a wide range of electronic engine control strategies. Measurements of spark plug ion current for approximating air/fuel ratio may be especially useful for use with low cost mixture control in small engine applications. Cylinder-to-cylinder mixture balancing in conjunction with a global exhaust gas oxygen sensor is another promising application of spark plug ion current measurement.</div>
Procalcitonin in Early Detection of Postoperative Complications (1998)

Procalcitonin presents itself as a new parameter of infection and sepsis. In the postoperative period PCT seems to be an interesting marker of early prediction of infective complications when high postoperative levels are found. Under routine conditions procalcitonin is a valid reproducible and detectable parameter.
Glycerol Disrupts Tight Junction—Associated Actin Microfilaments, Occludin, and Microtubules in Sertoli Cells (2000)

Intratesticular injections of glycerol have been shown to result in a marked and prolonged reduction of spermatogenesis, accompanied by increased permeability of the blood-testis barrier. Because the permeability of the blood-testis barrier is regulated by Sertoli cell tight junctions, and tight junction organization is regulated by the cytoskeleton, we undertook to examine the effects of glycerol treatment on cytoskeletal actin microfilaments and microtubules, and on the tight junction protein, occludin, in Sertoli cells. Adult rats received a single intratesticular injection of either saline (controls) or a 10% glycerol solution. At 24 hours and 7, 15, and 21 days after injection, testes were collected and prepared for routine histology, cryosectioning, or whole seminiferous tubule immunohistochemical staining; and the preparations were viewed by light and confocal microscopy. In saline-injected testes, Sertoli cells had a cytoskeletal and junctional organization that resembled that of normal testes. F-actin microfilaments, located in the basal region, were arranged in regular bundles or chords that circumscribed the perimeter of each Sertoli cell at the level of the tight junction. Occludin colocalized with tight junction-associated actin filament distribution and microtubules formed a geometric array associated with spermatogenic cells. In contrast, in glycerol-treated Sertoli cells, microfilament and microtubule organization and occludin distribution were partially or completely disrupted. From these results we conclude that glycerol treatment either directly or indirectly disrupts tight junction-associated F-actin and occludin and tubulin organization in rat Sertoli cells. Perturbation of the tight junction-associated proteins could explain the increase in permeability of the blood-testis barrier observed after glycerol treatment. Impaired spermatogenesis following glycerol treatment is likely a consequence of a leaky blood testis barrier and disrupted Sertoli cell cytoskeleton. Glycerol injections may serve as a useful tool in studying the relationship between cytoskeletal organization and the stabilization of Sertoli-Sertoli cell junctions.
Effect of feeding <i>Bacillus subtilis natto</i> fermentation product on milk production and composition, blood metabolites and rumen fermentation in early lactation dairy cows (2011)

This experiment was conducted to determine the effect of Bacillus subtilis natto fermentation product supplementation on blood metabolites, rumen fermentation and milk production and composition in early lactation dairy cows. Thirty-six multiparous Holstein cows (DIM = 29 ± 6 days, parity = 2.8 ± 1.1) were blocked by DIM and parity and then randomly assigned to three treatments (12 per treatment) in a 9-week trial. Cows in control, DFM1 and DFM2 were fed TMR diets supplemented with 0, 6 and 12 g of B. subtilis natto solid-state fermentation product per day per cow respectively. Plasma non-esterified fatty acids were lower (p = 0.03) in DFM1 and DFM2 compared with control cows (633 and 639 vs. 685 μm). Ruminal propionate increased (23.9 vs. 26.3 and 26.9/100 mol, control vs. DFM1 and DFM2 respectively) and acetate decreased (64.2 vs. 62.7 and 62.1/100 mol, control vs. DFM1 and DFM2 respectively) with increasing B. subtilis natto fermentation product supplementation. DMI of the cows in three treatments was not affected by B. subtilis natto fermentation product supplementation, but milk yield was 3.1 and 3.2 kg/day higher for DFM1 and DFM2 than that for control cows on average across the 9-week trial, and significant differences were observed during weeks 5-9 of the trial, which resulted in 9.5% and 11.7% increase in feed efficiency. B. subtilis natto fermentation product supplementation did not affect milk fat percentage and protein yield but increased (p < 0.05) milk fat yield and lactose percentage (p < 0.01) and tended to decrease protein percentage (p = 0.06). The findings show that B. subtilis natto fermentation product was effective in increasing lactation performance of early lactation dairy cows possibly by altering the rumen fermentation pattern without any negative effects on blood metabolites.
Let’s Share: A Game-Theoretic Framework for Resource Sharing in Mobile Edge Clouds (2020)

Mobile edge computing seeks to provide resources to different delay-sensitive applications. This is a challenging problem as an edge cloud-service provider may not have sufficient resources to satisfy all resource requests. Furthermore, allocating available resources optimally to different applications is also challenging. Resource sharing among different edge cloud-service providers can address the aforementioned limitation as certain service providers may have resources available that can be “rented” by other service providers. However, edge cloud service providers can have different objectives or <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">utilities</i> . Therefore, there is a need for an efficient and effective mechanism to share resources among service providers, while considering the different objectives of various providers. We model resource sharing as a multi-objective optimization problem and present a solution framework based on <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Cooperative Game Theory</i> (CGT). We consider the strategy where each service provider allocates resources to its native applications first and shares the remaining resources with applications from other service providers. We prove that for a monotonic, non-decreasing utility function, the game is canonical and convex. Hence, the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">core</i> is not empty and the grand coalition is stable. We propose two algorithms, <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Game-theoretic Pareto optimal allocation</i> (GPOA) and <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Polyandrous-Polygamous Matching based Pareto Optimal Allocation</i> (PPMPOA) that provide allocations from the core. Hence the obtained allocations are <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Pareto</i> optimal and the grand coalition of all the service providers is stable. Experimental results confirm that our proposed resource sharing framework improves utilities of edge cloud-service providers and application request satisfaction.
ONC201 Exhibits Mutation-Independent Efficacy with Superior Potency in Non-Hodgkin Lymphoma and Multiple Myeloma (2015)

Abstract ONC201 is a first-in-class small molecule inducer of the integrated stress response that is currently in phase II clinical trials in select advanced cancers with promising early clinical results. The efficacy of this novel agent has been demonstrated in numerous preclinical advance cancer models in multiple indications with an exceptional safety profile that has translated well to the clinic. To determine the preclinical sensitivity profile of ONC201 in cancer, we performed an in vitro efficacy screen across >1,000 human cancer cell lines that represent a diverse array of tumor types and genetic aberrations. Sensitivity profiling was assessed by cell viability assays using dose responses curves at concentrations up to 20uM and at 72 hours post-treatment. Ranking the sensitivity dataset by tumor type, non-Hodgkin's lymphomas and multiple myeloma were the most sensitive tumor type to ONC201. The mutation-agnostic efficacy that is most pronounced in lymphomas and multiple myeloma is in accordance with the recent findings that ONC201 induces the integrated stress response through a novel target to trigger is downstream late apoptotic effects. B-cell malignancies are particularly susceptible to induction of apoptosis via the integrated stress response, as they have relatively high basal activation of this pathway due to ER stress conferred by immunoglobulin production. Confirmatory studies revealed that multiple myeloma cell lines indeed possess pronounced sensitivity with nanomolar GI50s, unlike most other tumor types, that is particularly encouraging given the systemic concentrations observed in the first-in-man study. Together, these studies suggest specific advanced cancer indications, such as non-Hodgkin's lymphoma and multiple myeloma, as promising lead indications for this novel agent that are being evaluated in phase II clinical trials. Disclosures Allen: Oncoceutics, Inc: Employment, Equity Ownership. Tarapore:Oncoceutics, Inc: Employment, Equity Ownership.