gebhart/patrepeval huggingface dataset:
Plasmid stability and α‐amylase production in batch and continuous cultures of <i>Bacillus subtilis</i> TN106[pAT5] (1989)

Cell growth and enzyme (alpha-amylase) production characteristics of Bacillus subtilis TN106 containing the recombinant plasmid pAT5 are investigated in batch and continuous cultures using a defined medium with glucose as the limiting nutrient. The batch culture studies demonstrate that the recombinant plasmid, reported earlier(1) to be stably maintained in the host, suffers from segregational and structural instabilities. The structural instability of this strain occurred during culture storage and can be eliminated in bioreactor experiments by using a modified inoculum preparation procedure. Such elimination allows an unbiased investigation of segregational instability via continuous culture studies. Such studies conducted with this fast growing microorganism, in the absence of antibiotic selection pressure, indicate a very efficient glucose utilization (very low residual glucose concentrations) over a wide range of dilution rates (0.16 h(-1) - 0.94 h(-1)). The nearly time-invariant and low residual glucose concentrations at each such dilution rate enable convenient estimation of growth parameters of the host and recombinant cells and frequency of segregational instability from transients in the resulting mixed cultures. The specific alpha-amylase activity exhibits an inverse relationship to the specific growth rate of recombinant cells. The growth of recombinant cells is not affected by the presence of antibiotic (kanamycin). The growth advantage of host cells over recombinant cells diminishes with increasing dilution rate.
Control of Alkanethiolate Monolayer Structure Using Vapor-Phase Annealing (2003)

We describe an annealing procedure for self-assembled monolayers (SAMs) that uses vapor-phase molecules to modify the local domain structure. Existing SAMs of decanethiolate on Au{111} were annealed using vapor-phase dodecanethiol molecules, so that the original and newly introduced molecules could be distinguished using scanning tunneling microscopy (STM). Molecules deposited from the vapor phase inserted at existing monolayer defect sites and domain boundaries, and at substrate step edges forming discrete network-like domains. The SAM molecular lattice can be preserved across molecular terrace boundaries between the decanethiolate and dodecanethiolate domains. Candidate molecular electronic component molecules were inserted from solution in the decanethiolate matrix as isolated molecules. These inserted molecules could then be surrounded by dodecanethiolate molecules introduced from the vapor phase, thus demonstrating a method for controlling the local environment of inserted molecules.
α<sub>7</sub>β<sub>1</sub>-Integrin regulates mechanotransduction and prevents skeletal muscle injury (2006)

Alpha7beta1-integrin links laminin in the extracellular matrix with the cell cytoskeleton and therein mediates transduction of mechanical forces into chemical signals. Muscle contraction and stretching ex vivo result in activation of intracellular signaling molecules that are integral to postexercise injury responses. Because alpha7beta1-integrin stabilizes muscle and provides communication between the matrix and cytoskeleton, the role of this integrin in exercise-induced cell signaling and skeletal muscle damage was assessed in wild-type and transgenic mice overexpressing the alpha7BX2 chain. We report here that increasing alpha7beta1-integrin inhibits phosphorylation of molecules associated with muscle damage, including the mitogen-activated protein kinases (JNK, p38, and ERK), following downhill running. Likewise, activation of molecules associated with hypertrophy (AKT, mTOR, and p70(S6k)) was diminished in mice overexpressing integrin. While exercise resulted in Evans blue dye-positive fibers, an index of muscle damage, increased integrin protected mice from injury. Moreover, exercise leads to an increase in alpha7beta1 protein. These experiments provide the first evidence that alpha7beta1-integrin is a negative regulator of mechanotransduction in vivo and provides resistance to exercise-induced muscle damage.
Non-destructive evaluation of wind turbine blades using an infrared camera (1999)

The use of a digital infrared as a non-destructive evaluation thermography camera (NDE) tool was ex- plored in two separate wind turbine blade fatigue tests. The fwst test was a fatigue test of part of a 13.1 meter wood-epoxy-composite blade. The second test was on a 4.25 meter pultruded fiber glass blade section driven at several mechanical resonant frequencies. The digital infrared camera can produce images of either the static temperature distribution on the surface of the specimen, or the dynamic temperature distribution that is in phase with a specific frequency on a vibrating specimen. The dynamic temperature distribution (due to thermoplastic effects) gives a measure of the sum of the principal stresses at each point on the surface. In the wood- epoxy-composite blade fatigue test, the point of ultimate failure was detected long before failure occurred. The mode shapes obtained with the digital infrared camera, from the resonant blade tests, were in very good agree- ment with the finite-element calculations. In addition, the static temperature images of the resonating blade showed two areas that contained cracks. Close-up dy- namic inf%red images of these areas showed the crack structure that agreed with subsequent dye-penetrant analysis.
Confined Polymerization in Porous Organic Frameworks with an Ultrahigh Surface Area (2012)

Polymers framed: Interpenetrated nanocomposites were formed by polymerization of acrylonitrile in a ultrahigh surface area (>5000 m2 g−1) porous framework. The resulting material realizes a hyperextended interface with uniform interdigitation of the two structures at the size limit of the individual molecular moieties. The confined poly(acrylonitrile) chains could be transformed into light-absorbing polyconjugated ladder polymers. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Differing definitions of manufacturer. (1998)

Medical device manufacturers marketing in the United States (US) and Europe must comply with different labelling requirements concerning the indication of manufacturer on the label. These differences are increased if US labelling requirements are compared with the practice of own-brand labelling under the European medical device Directives. This article discusses the differing requirements.
Photoacoustic imaging endoscope (2009)

We currently present a design concept for a photoacoustic imaging endoscope as well as some preliminary experimental results. The device is based on the generation of acoustic waves in tissue by short laser pulses and the sensing of these waves with a thin, optical Fabry-Pérot etalon. The entire device is designed to be mounted on the tip of a needle to deliver high-resolution photoacoustic imaging for minimally-invasive clinical applications such as diagnosing kidney disease and guiding laparoscopic surgery.
Analysis of Genetic Diversity and Population Structure of Sesame Accessions from Africa and Asia as Major Centers of Its Cultivation (2016)

Sesame is an important oil crop widely cultivated in Africa and Asia. Understanding the genetic diversity of accessions from these continents is critical to designing breeding methods and for additional collection of sesame germplasm. To determine the genetic diversity in relation to geographical regions, 96 sesame accessions collected from 22 countries distributed over six geographic regions in Africa and Asia were genotyped using 33 polymorphic SSR markers. Large genetic variability was found within the germplasm collection. The total number of alleles was 137, averaging 4.15 alleles per locus. The accessions from Asia displayed more diversity than those from Africa. Accessions from Southern Asia (SAs), Eastern Asia (EAs), and Western Africa (WAf) were highly diversified, while those from Western Asia (WAs), Northern Africa (NAf), and Southeastern Africa (SAf) had the lowest diversity. The analysis of molecular variance revealed that more than 44% of the genetic variance was due to diversity among geographic regions. Five subpopulations, including three in Asia and two in Africa, were cross-identified through phylogenetic, PCA, and STRUCTURE analyses. Most accessions clustered in the same population based on their geographical origins. Our results provide technical guidance for efficient management of sesame genetic resources in breeding programs and further collection of sesame germplasm from these different regions.
Dimensions of Xf virus from its rotational and translational diffusion coefficients (1980)

Measurements on monodisperse preparations of the filamentous virus Xf give 17.3 +/- 0.6 s-1 for its rotational diffusion coefficient in 1 mM sodium phosphate, pH 7.5 at 20 degrees C and (2.53 +/- 0.06) X 10(-8) cm2 s-1 for its translational diffusion coefficient in 0.15 M NaCl and 0.015 M sodium phosphate, pH 7, at 25 degrees C. These coefficients yield a length of 980 +/- 30 nm and a diameter of 7.2 +/- 1.5 nm for the virus in solution when used to solve simultaneously the two Broersma equations for the diffusion of rigid rods.
Preparation, characterization and catalytic properties of nickel aluminate nanoparticles: A comparison between conventional and microwave method (2014)

In the present work, synthesis of nickel aluminate using Opuntia dilenii haw as plant extract by a microwave combustion method (MCM) and its comparison with the conventional combustion method (CCM) is investigated. O. dilenii haw plant extract simplifies the process, provides an alternative process for a simple and an economical synthesis. The absence of surfactant has led to a simple, cheap and fast method of synthesis of NiAl2O4 nanoparticles. The as-synthesized NiAl2O4 nanoparticles were characterized by X-ray diffraction (XRD) studies, Fourier transform infrared spectroscopy (FT-IR) studies, high resolution scanning electron microscopy (HR-SEM), energy dispersive X-ray analysis (EDX), high resolution transmission electron microscopy (HR-TEM), diffuses reflectance spectroscopy (DRS), and Brunauer Emmett Teller (BET) surface area analysis. The XRD results confirmed the formation of the cubic phase NiAl2O4. The formation of pure nickel aluminate phase was confirmed by FT-IR. The formation of NiAl2O4 nanoparticles was confirmed by HR-SEM and HR-TEM and their possible formation mechanisms were also proposed. MCM could produce NiAl2O4 with uniform size and well-defined shape with crystallinity. The optical property was determined by DRS. NiAl2O4 prepared by the microwave combustion method was found to possess a higher surface area, lower crystallite size than the NiAl2O4 nanoparticles prepared by the conventional combustion method, which in turn has led to the improved performance toward the selective oxidation of benzyl alcohol to benzaldehyde.
Direct observation of standing wave formation at surface steps using scanning tunneling spectroscopy (1993)

Using scanning tunneling microscopy and spectroscopy, we have observed the formation of standing waves by the scattering of surface state electrons at steps and defects on the Au(111) surface. From the periods, peak positions, and intensities of the standing waves, the energy dispersion of the surface states, the scattering phase shifts as a function of energy and crystallographic direction, and coherence lengths could be determined.
Effect of tip geometry on contrast and spatial resolution of the near-field microwave microscope (2006)

The near-field scanning microwave microscope (NSMM) can quantitatively image materials properties at length scales far shorter than the free space wavelength (λ). Here we report a study of the effect of tip geometry on the NSMM signals. This particular NSMM utilizes scanning tunneling microscopy (STM) for distance-following control. We systematically examined many commercially available STM tips and found them to have a conical structure on the macroscopic scale, with an embedded sphere (of radius rsphere) at the apex of the tip. The rsphere values used in the study ranged from 0.1to12.6μm. Tips with larger rsphere show good signal contrast [as measured by the frequency shift (Δf) signal between tunneling height and 2μm away from the sample] with NSMM. For example, the tips with rsphere=8μm give signal contrast of 1000kHz compared to 85kHz with a tip of rsphere=0.55μm. However, large rsphere tips distort the topographic features acquired through STM. A theoretical model is used to understand the tip-to-sample interaction. The model quantitatively explains the measured change in quality factor (Q) as a function of height over bulk copper and silicon samples.
High-throughput, luciferase-based reverse genetics systems for identifying inhibitors of Marburg and Ebola viruses (2014)

Marburg virus (MARV) and Ebola virus (EBOV), members of the family Filoviridae, represent a significant challenge to global public health. Currently, no licensed therapies exist to treat filovirus infections, which cause up to 90% mortality in human cases. To facilitate development of antivirals against these viruses, we established two distinct screening platforms based on MARV and EBOV reverse genetics systems that express secreted Gaussia luciferase (gLuc). The first platform is a mini-genome replicon to screen viral replication inhibitors using gLuc quantification in a BSL-2 setting. The second platform is complementary to the first and expresses gLuc as a reporter gene product encoded in recombinant infectious MARV and EBOV, thereby allowing for rapid quantification of viral growth during treatment with antiviral compounds. We characterized these viruses by comparing luciferase activity to virus production, and validated luciferase activity as an authentic real-time measure of viral growth. As proof of concept, we adapt both mini-genome and infectious virus platforms to high-throughput formats, and demonstrate efficacy of several antiviral compounds. We anticipate that both approaches will prove highly useful in the development of anti-filovirus therapies, as well as in basic research on the filovirus life cycle.
Amylin: A Novel Action in the Brain to Reduce Body Weight* (2000)

Amylin is a 37-amino acid peptide hormone that is co-secreted with insulin by pancreatic B cells in response to a nutrient stimulus (e.g., during meals). To test the hypothesis that amylin acts within the brain to reduce long-term food intake and body weight, we examined the effects of acute and chronic 3rd-ventricular (i3vt) infusion of low doses of amylin on food intake and body weight in rats. In one experiment, separate groups of ad lib-fed male Long Evans rats were given one i3vt infusion (3 microl over 30 s) of synthetic cerebrospinal fluid vehicle or 1 to 100 pmol amylin, and food intake and body weight were monitored for 7 days. Amylin potently and dose-dependently reduced 1-h food intake, with all doses producing significant reductions. The largest dose (100 pmol) significantly reduced 24-h intake by over 30%. The effect was persistent in that both 7-day cumulative food intake and body weight change were significantly decreased over the 7 days following a single injection of 100 pmol of amylin. Other groups of rats received continuous i3vt infusion (0.5 microl/h volume) of saline or 2.0 pmol/h amylin via osmotic minipumps over 10 days. Food intake over the 10-day infusion was significantly suppressed in amylin-treated rats as compared to that of controls. Consequently, by the 4th day of infusion, amylin rats weighed significantly less than baseline relative to saline controls, and this difference persisted throughout the remainder of the infusion period. At sacrifice (Day 10), the percent of body weight from retroperitoneal fat depots was significantly lower in the amylin-treated rats, indicative of a reduction of total body adiposity. In summary, the results support the hypothesis that amylin acts as a signal to the brain contributing to the maintenance of long-term energy balance.
A fast multiscale framework for data in high-dimensions: Measure estimation, anomaly detection, and compressive measurements (2012)

Data sets are often modeled as samples from some probability distribution lying in a very high dimensional space. In practice, they tend to exhibit low intrinsic dimensionality, which enables both fast construction of efficient data representations and solving statistical tasks such as regression of functions on the data, or even estimation of the probability distribution from which the data is generated. In this paper we introduce a novel multiscale density estimator for high dimensional data and apply it to the problem of detecting changes in the distribution of dynamic data, or in a time series of data sets. We also show that our data representations, which are not standard sparse linear expansions, are amenable to compressed measurements. Finally, we test our algorithms on both synthetic data and a real data set consisting of a times series of hyperspectral images, and demonstrate their high accuracy in the detection of anomalies.
Solid acid-reduced graphene oxide nanohybrid for enhancing thermal stability, mechanical property and flame retardancy of polypropylene (2015)

PMoA enhances the radical trapping effect of graphene.
An Economic Analysis On Intermediate Microeconomics: An Ordered Probit Model (2005)

An ordered probit model is utilized in this study to determine primary factors leading to academic success among undergraduate students taking intermediate microeconomics. Using a sample of 169 students enrolled in intermediate microeconomics during the 2003-2004 academic year, it is found that individual student cumulative grade point average or total score on the Scholastic Aptitude Test along with choice of academic major influence the expected grade in intermediate microeconomics.
Achievements and challenges in the integration, reuse and synthesis of vegetation plot data (2016)

Abstract Aims I aim to review vegetation plot data discovery, the major international efforts to integrate these data, some of the remaining barriers to data integration, reuse and synthesis and how they can be overcome, and some of the emergent issues associated with data attribution and acknowledgement for data providers, users and aggregators. Results Vegetation plot data from 231 databases containing over three million plot records can be discovered via the metadata catalogue of the Global Index of Vegetation‐Plot Databases (GIVD). Major efforts to integrate data at national and international scales are well underway, including the North American VegBank, the European Vegetation Archive, the Botanical Information and Ecology Network (BIEN) and sPlot. Barriers to data reuse and synthesis remain: the most important are missing or incorrect geographic coordinates (geo‐coordinates) and inconsistencies in plant names. Many scientific journals now require the data underpinning published results to be archived in a publically accessible location via a digital object identifier (DOI). Such policies may be at odds with those of vegetation plot databases and funding agencies. The linkage between the New Zealand National Vegetation Survey Databank and an institutional data repository illustrates one solution to satisfying journal requirements to make data publically available, while retaining a direct linkage to the source data archive. Conclusions Although further progress needs to be made in digitising, publishing and integrating vegetation plot data, many once insurmountable barriers are rapidly being overcome. Developing effective solutions to the problems posed by changing taxonomic concepts in space and time is likely the most urgent requirement. Although changing journal requirements may result in vegetation plot data being archived in some form for a specific publication, this does not provide the integration required to enable data reuse and synthesis. For vegetation scientists, a recommended best practice is to archive plot data in an established vegetation plot repository as a first step, and when required, provide versioned data or summaries to meet journal requirements in a suitable repository with a clear linkage to the vegetation plot repository. The concepts outlined in this paper have wide‐ranging implications for other types of ecological data.
Wellsite Applications of Integrated MWD and Wellsite Data (1986)

ABSTRACT The advent of a wellsite computing center utilizing a common data base for surface and downhole measurements while drilling, provides new answers regarding safety, drilling efficiency and geological evaluation. New applications have been developed utilizing the synergistic combination of measurements made at the surface during drilling coupled with MWD measurements. These applications have demonstrated significant improvements in drilling efficiency, geological evaluation and safety. On-line drilling programs have been developed to improve hydraulics, rate of penetration and solve drilling mechanics problems. Formation evaluation software provides an immediate indication of water saturation. Safety is enhanced by on-line plotting of pressure trends from MWD resistivity, formation strength analysis and other pressure parameters. In addition real time computer programs improve safety performance of the rig crew while tripping and while controlling kicks.
Role of stem cells in large animal genetic engineering in the TALENs–CRISPR era (2013)

The establishment of embryonic stem cells (ESCs) and gene targeting technologies in mice has revolutionised the field of genetics. The relative ease with which genes can be knocked out, and exogenous sequences introduced, has allowed the mouse to become the prime model for deciphering the genetic code. Not surprisingly, the lack of authentic ESCs has hampered the livestock genetics field and has forced animal scientists into adapting alternative technologies for genetic engineering. The recent discovery of the creation of induced pluripotent stem cells (iPSCs) by upregulation of a handful of reprogramming genes has offered renewed enthusiasm to animal geneticists. However, much like ESCs, establishing authentic iPSCs from the domestic animals is still beset with problems, including (but not limited to) the persistent expression of reprogramming genes and the lack of proven potential for differentiation into target cell types both in vitro and in vivo. Site-specific nucleases comprised of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regulated interspaced short palindromic repeats (CRISPRs) emerged as powerful genetic tools for precisely editing the genome, usurping the need for ESC-based genetic modifications even in the mouse. In this article, in the aftermath of these powerful genome editing technologies, the role of pluripotent stem cells in livestock genetics is discussed.