gebhart/patrepeval huggingface dataset:
P2 and P2′ Residues Are Important for the Efficient Proteolysis of Factor VIIIa at Arg336 Catalyzed by Activated Protein C. (2007)

Abstract Activated Protein C (APC) is an anticoagulant serine protease that proteolytically inactivates the coagulation cofactors, factors (F) Va and VIIIa. FVIIIa is a non-covalent heterotrimer consisting of A1, A2 and A3-C1-C2 subunits. APC-catalyzed inactivation of FVIIIa results from proteolysis at the P1 residues Arg336 and Arg562 within the A1 and A2 subunits, respectively, with cleavage at Arg336 representing the dominant reaction. We recently showed that replacement of the P4-P3′ residues surrounding the Arg336 site with the corresponding residues flanking Arg562 resulted in an ∼100-fold reduction in cleavage rate at Arg336 (Varfaj et al, J. Biol. Chem.2007). Comparison of the P4-P3′ residues at the slow-reacting site in FVIIIa (Arg562) with the corresponding residues at the fast-reacting site in FVa (Arg506) revealed that these sequences differ primarily at the P2 and P2′ positions. This observation suggested an important contribution by these residues to cleavage efficiency. The role of the P2 and P2′ residues in the proteolysis of FVIIIa by APC was investigated by preparing recombinant FVIII proteins possessing mutations at Leu335 (P2) and Lys338 (P2′) flanking the P1 Arg336. B-domainless FVIII proteins were stably expressed in BHK cells and purified. Leu335Arg and Leu335Gln mutants were constructed based upon the P2 residues preceding Arg506 in FVa and Arg562 in FVIIIa, respectively. The Lys338Ile mutant was based upon the P2′ residue following Arg506 in FVa. Specific activity values for all FVIII variants were similar to wild type (WT) FVIII. APC-catalyzed inactivation rates for FVIIIa were determined using a FXa generation assay, and rates for proteolysis at the scissile bonds within the A1 and A2 subunits were determined by SDS-PAGE and Western blotting. Rates for APC-catalyzed cleavage of the A1 subunit for the FVIIIa variants Leu335Arg and Leu335Gln were reduced ∼2- and ∼4-fold, respectively, as compared to WT. However, the rate of cleavage of the A1 subunit in the Lys338Ile FVIIIa variant was enhanced ∼3-fold compared to WT. Cleavage rates at Arg562 in all the variants were unaffected by mutation at either residue 335 or 338. These relative values for rates of proteolysis paralleled the observed rates for inactivation of the FVIIIa forms. Overall, these results suggest that both P2 and P2′ residues are important in the efficient proteolysis at Arg336 in FVIIIa. Furthermore, Leu appears more optimal than either Arg or Gln at the P2 position, whereas Ile is preferred over Lys at the P2′ position in this macromolecular substrate for APC.
Structural Health Monitoring of Steel Pipes under Different Boundary Conditions and Choice of Signal Processing Techniques (2012)

Guided wave technique is an efficient method for monitoring structural integrity by detecting and forecasting possible damages in distributed pipe networks. Efficient detection depends on appropriate selection of guided wave modes as well as signal processing techniques. Fourier analysis and wavelet analysis are two popular signal processing techniques that provide a flexible set of tools for solving various fundamental problems in science and engineering. In this paper, effective ways of using Fourier and Wavelet analyses on guided wave signals for detecting defects in steel pipes are discussed for different boundary conditions. This research investigates the effectiveness of Fourier transforms and Wavelet analysis in detecting defects in steel pipes. Cylindrical Guided waves are generated by piezo-electric transducers and propagated through the pipe wall boundaries in a pitch-catch system. Fourier transforms of received signals give information regarding the propagating guided wave modes which helps in detecting defects by selecting appropriate modes that are affected by the presence of defects. Continuous wavelet coefficients are found to be sensitive to defects. Several types of mother wavelet functions such as Daubechies, Symlet, and Meyer have been used for the continuous wavelet transform to investigate the most suitable wavelet function for defect detection. This research also investigates the effect of different boundary conditions on wavelet transforms for different mother wavelet functions.
An improved implementation method of AHB busmatrix (2005)

The multi-layer AHB BusMatrix (ML-AHB BusMatrix) proposed by ARM allows parallel access paths between multiple masters and slaves in a system. But, there is one clock cycle delay for each master in the ML-AHB BusMatrix of ADK (AMBA/spl trade/ design kit) whenever the master restarts the transactions or changes the slave layers because of the input stage and arbitration logic implemented with Moore type. In this paper, we present one way to improve the ML-AHB BusMatrix structure of ADK. With the elimination of the input stage and some restrictions on the arbitration scheme, we could not only take away the one clock cycle delay but also reduce the area overhead and power consumption. Experimental results show that the one clock cycle delay is removed. Besides the average dynamic power, static power, total area overhead and clock period of ameliorated ML-AHB BusMatrix are reduced by 42%, 64%, 33% and 33% respectively, compared with the ML-AHB BusMatrix of ADK.
Performance Modeling of a Thrust Vectoring Device for Hall Effect Thrusters (2009)

International audience
Detection of Amphipathic Viral Peptide on Screen-Printed Electrodes by Liposome Rupture Impact Voltammetry (2017)

Detection of infectious viruses and disease biomarkers is of utmost importance in clinical screening for effective identification and treatment of diseases. We demonstrate here the use of liposome rupture impact voltammetry for the qualitative detection of model amphipathic viral peptide on a screen-printed electrode. This novel, proof-of-concept method was proposed for the quick and reliable detection of viruses by nonfaradaic liposome rupture impact voltammetry with the aid of 1,2-dioleoyl-sn-glycero-3-phosphocholine liposomes. This provides an avenue for the development of future on-site, point-of-care detection devices for medical and biological applications.
Thermal annealing of deep ultraviolet (193 nm) induced compaction in fused silica (1998)

Different 1995–1996 ultraviolet (UV) grade experimental fused silica samples were evaluated for their resistance to UV-induced compaction at 193 nm under elevated sample temperature conditions. We found that the compaction rate decreases with increasing sample temperature. Compaction recoveries were observed at temperatures as low as 120 °C, this suggests that the recovery of UV-induced compaction in fused silica is a thermally excited process with a low activation energy. Isothermal annealing experiments were performed on two different sets of precompacted fused silica samples. An activation energy of 0.13 eV was found for both cases.
Subchondral bone in osteoarthritis: insight into risk factors and microstructural changes (2013)

Osteoarthritis (OA) is a major cause of disability in the adult population. As a progressive degenerative joint disorder, OA is characterized by cartilage damage, changes in the subchondral bone, osteophyte formation, muscle weakness, and inflammation of the synovium tissue and tendon. Although OA has long been viewed as a primary disorder of articular cartilage, subchondral bone is attracting increasing attention. It is commonly reported to play a vital role in the pathogenesis of OA. Subchondral bone sclerosis, together with progressive cartilage degradation, is widely considered as a hallmark of OA. Despite the increase in bone volume fraction, subchondral bone is hypomineralized, due to abnormal bone remodeling. Some histopathological changes in the subchondral bone have also been detected, including microdamage, bone marrow edema-like lesions and bone cysts. This review summarizes basic features of the osteochondral junction, which comprises subchondral bone and articular cartilage. Importantly, we discuss risk factors influencing subchondral bone integrity. We also focus on the microarchitectural and histopathological changes of subchondral bone in OA, and provide an overview of their potential contribution to the progression of OA. A hypothetical model for the pathogenesis of OA is proposed.
Using a body sensor network to measure the effect of fatigue on stair climbing performance (2012)

In terms of self-rated health, the most important activities of daily living are those involving mobility. Of these activities stair climbing is regarded as the most strenuous. A loss of stair climbing ability with age is normally associated with a loss of muscle strength and power, while other factors that influence muscle function, such as fatigue, are often not taken into account. So far no research has been published on how long-lasting fatigue affects activities of daily living, despite the fact that it has been repeatedly proven, in laboratory settings, to influence muscle force production over long periods of time. Technological advances in body sensor networks (BSNs) now provide a method to measure performance during complex real-life situations. In this study the use of a BSN was explored to investigate the effects of long-lasting fatigue on stair climbing performance in 20 healthy adults. Stair climbing performance was measured before and after a fatiguing protocol using a BSN. Performance was defined by temporal and spatial parameters. Long-lasting fatigue was successfully induced in all participants using an exercise protocol. The BSN showed that post-exercise fatigue did not influence stair climbing times (p > 0.2) and no meaningful changes in joint angles were found. No effect on overall stair climbing performance was found, despite a clear presence of long-lasting fatigue. This study shows that physiological paradigms can be further explored using BSNs. Ecological validity of lab-based measurements can be increased by combining them with BSNs.
Electrical measurement of non-destructively p-type doped graphene using molybdenum trioxide (2011)

We demonstrate effective non-destructive p-type doping of graphene via surface modification with molybdenum trioxide (MoO3) thin film using electrical transport measurements. The p-type doping via MoO3 modification of graphene leads to the downward shift of Fermi level towards the valence band. MoO3 modified graphene retains its high charge carrier mobility, facilitating the observation of quantum Hall effect. In-situ ultraviolet photoelectron spectroscopy studies also show that air exposure of MoO3 modified graphene reduces the doping efficiency.
Vaginal temperature sensing using UHF radio telemetry (1996)

User-induced errors are common when women repetitively employ conventional probe type thermometers to chart their basal body temperatures in an effort to indicate ovulation. An alternative technique employing a two-part telemetric thermometer is proposed, with low-power, SAWR-controlled UHF radio as the transmission medium. Worn overnight in the vagina, the 1 microW erp telemetry transmitter sends pulse modulated data continuously to a microcontroller in a nearby receiver; a real time clock enables programmable sampling and storage of the subject's temperature to 0.1 degrees C resolution. Initial clinical results indicate an enhanced performance compared to oral and axillary temperature trends taken by a mercury-in-glass thermometer. Polar plots of both the isolated and body-worn telemetry transmitter are presented; body induced attenuations of up to 30 dB were measured.
High-Bandwidth Feedback Control of Micromachined Tunneling Sensors (1995)

Abstract Displacement transducers based on quantum tunneling have been demonstrated for use in accelerometers, IR sensors, and other devices. An important characteristic of the tunneling transducer is that the tunneling current only occurs when the electrodes are separated by a gap of order 10 Å. Because of the requirement for a 10 Å gap between tunneling electrodes, a feedback control system is necessary to balance the signal forces to a very high degree of accuracy over the entire expected signal bandwidth. In this paper, we study several tunneling sensor control systems, with the goal of improving the control bandwidth. With our model analysis, a control bandwidth of 50 kHz is predicted, which is in excellent agreement with the measured bandwidth for these devices.
The Role of Surface Oxygen Vacancies in the NO<sub>2</sub> Sensing Properties of SnO<sub>2</sub> Nanocrystals (2008)

SnO2 nanocrystals were prepared by injecting a hydrolyzed methanol solution of SnCl4 into a tetradecene solution of dodecylamine. The resulting materials were annealed at 500 °C, providing 6−8 nm nanocrystals. The latter were used for fabricating NO2 gas sensing devices, which displayed remarkable electrical responses to as low as 100 ppb NO2 concentration. The nanocrystals were characterized by conductometric measurements, X-ray photoelectron spectroscopy (XPS), electron paramagnetic resonance (EPR), and cathodoluminescence (CL) spectroscopy. The results, interpreted by means of molecular modeling in the frame of the density functional theory (DFT), indicated that the nanocrystals contain topographically well-defined surface oxygen vacancies. The chemisorption properties of these vacancies, studied by DFT modeling of the NO2/SnO2 interaction, suggested that the in-plane vacancies facilitate the NO2 adsorption at low operating temperatures, while the bridging vacancies, generated by heat treatment at 500 °C, enhance the charge transfer from the surface to the adsorbate. The behavior of the oxygen vacancies in the adsorption properties revealed a gas response mechanism in oxide nanocrystals more complex than the size dependence alone. In particular, the nanocrystals surface must be characterized by enhanced transducing properties for obtaining relevant gas responses.
"Charlie,"development of a light-weight, virtual reality trainer for the LSO community: time to make the leap toward immersive VR (2015)

Landing Signal Officers (LSOs) are the backbone of tailhook naval aviation. Currently, once a junior officer is selected from a squadron to become an LSO, that person typically will go through an entire workup cycle before going to the Initial Formal Ground Training (IFGT) course. This means that an LSO will undergo months of on-the-job training at sea and assume different roles needed to recover aircraft before that individual receives his/her first formal training during IFGT. At the center of IFGT is the LSO Trainer, Device 2H111, in which the LSO receives a series of six one-hour long sessions. For many LSOs, this will be the only interaction will this training simulator. The aim of this thesis was to develop and evaluate whether major training objectives for the 2H111 could be supported using a proof of concept, light-weight, portable VR trainer with a VR HMD as its display solution. Thesis work included feasibility testing of a Graphical User Interface and voice recognition integration into a simulation to facilitate both an individual and a team training environment. The result of the study is that technology has come far enough to support a commercial-off-the-shelf technology solution.
Constrained moments simulation of healthcare capital acquisitions (1997)

Summary form only as given. Two analytical techniques which evaluate capital acquisitions in healthcare are the method of generating system moments and Monte Carlo simulation. Generating system moments enables the user to determine an estimate of the expected cost from a user-supplied function. Furthermore, the user can determine, from a variance component analysis, the sensitivity of the user-supplied function to marginal changes in the random variables by expanding the function about its mean using the Taylor series expansion to the second order. Calculating the magnitude of partial derivatives of each random variable with respect to the user-supplied function indicates the relative importance of each random variable to the function. VARSIM is an interactive program which is used to calculate the system moments. An add-in program to an Excel spreadsheet is used to invoke a Monte Carlo simulation, whose results are useful for assessing potential risks associated with a capital investment. The results from VARSIM indicate that the expected cost per MRI exam is $350 with a standard deviation of $11.81. Operating hours are increased from 50 to 126 hours per week. The simulation results indicate that there is a 55.2 percent probability that this cost level will be achieved and ultimately its annual target number of exams. Thus, the systematic approach presented in this paper provides a solid basis which hospitals can use to perform a thorough assessment of their capital equipment needs, and thereby, present their findings in a more objective manner to decision-makers.
Comparative Analysis on Interference Suppressive Transmission Schemes for White Space Radio Access (2012)

With opening up of the TV white spaces for opportunistic access, designing a flexible physical layer (PHY) scheme has become an important focus for cognitive radio (CR) systems. Two possible PHY design solutions proposed are: generalized frequency division multiplexing (GFDM) and interference avoidance by partitioned frequency and time domain transmission (IA-PFT). Both of these methods extend the orthogonal frequency division multiplexing (OFDM) scheme to be applicable as a flexible CR PHY solution in a fragmented spectrum. In GFDM, introduction of pulse shaping filters reduces the out-of-band radiation of the opportunistic signals into the frequency band of the incumbent users; while in IA-PFT, simultaneous cancellation carrier insertion and time windowing technique suppress spectral leakage into the incumbent band of operation. In this paper, these two different approaches are compared in terms of interference suppression, transmission performance and processing complexity.
Synthesis of phase-pure and monodisperse iron oxide nanoparticles by thermal decomposition (2015)

Superparamagnetic iron oxide nanoparticles (SPIONs) are used for a wide range of biomedical applications requiring precise control over their physical and magnetic properties, which are dependent on their size and crystallographic phase. Here we present a comprehensive template for the design and synthesis of iron oxide nanoparticles with control over size, size distribution, phase, and resulting magnetic properties. We investigate critical parameters for synthesis of monodisperse SPIONs by organic thermal decomposition. Three different, commonly used, iron containing precursors (iron oleate, iron pentacarbonyl, and iron oxyhydroxide) are evaluated under a variety of synthetic conditions. We compare the suitability of these three kinetically controlled synthesis protocols, which have in common the use of iron oleate as a starting precursor or reaction intermediate, for producing nanoparticles with specific size and magnetic properties. Monodisperse particles were produced over a tunable range of sizes from approximately 2-30 nm. Reaction parameters such as precursor concentration, addition of surfactant, temperature, ramp rate, and time were adjusted to kinetically control size and size-distribution, phase, and magnetic properties. In particular, large quantities of excess surfactant (up to 25 : 1 molar ratio) alter reaction kinetics and result in larger particles with uniform size; however, there is often a trade-off between large particles and a narrow size distribution. Iron oxide phase, in addition to nanoparticle size and shape, is critical for establishing magnetic properties such as differential susceptibility (dm/dH) and anisotropy. As an example, we show the importance of obtaining the required size and iron oxide phase for application to Magnetic Particle Imaging (MPI), and describe how phase purity can be controlled. These results provide much of the information necessary to determine which iron oxide synthesis protocol is best suited to a particular application.
Thymidylate synthetase from amethopterin- resistant Lactobacillus casei (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThymidylate synthetase from amethopterin- resistant Lactobacillus caseiFrank M. Huennekens, R. B. Dunlap, and Nigel G. L. HardingCite this: Biochemistry 1971, 10, 1, 88–97Publication Date (Print):January 5, 1971Publication History Published online1 May 2002Published inissue 5 January 1971https://doi.org/10.1021/bi00777a014RIGHTS & PERMISSIONSArticle Views109Altmetric-Citations185LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (3 MB) Get e-Alerts Get e-Alerts
Imaging nicotinic acetylcholine receptors with fluorine-18-FPH, an epibatidine analog. (1997)

These results demonstrate the feasibility of imaging nAChRs in vivo. Fluorine-18-FPH appears to be a suitable tracer to study nAChRs in the human brain.
Monograph of the Monistriini and Petasidini (Orthoptera : Pyrogomorphidae) (1985)

A numerical taxonomic study is made of the endemic Australian tribe Monistriini auct, in relation to the African and Indian Poekilocerini. It is concluded that the genera Petasida and Scutillya are phenetically as removed from the remainder of the Monistriini auct. as the Poekilocerini are from either; a new tribe Petasidini is erected to accommodate them. The two Australian tribes are revised comprehensively, with descriptions, figures, and keys of the 14 species. One new genus, Parastria, with new species P. reticulata, and a new species Monistria consobrina, are recognized. Extensive intraspecific geographical variation is described for most of the species and numerous geographical races discriminated. Distributions are documented by means of detailed maps. Biological information is presented when available. A small percentage of macropterous individuals occurs in several species of the mainly brachypterous genus Monistria; evidence is presented for regarding these as in part a kentromorphic phenomenon, but gregariousness has not been observed. Any one species has a limited range of host plants, many of which contain toxins. It is suggested that these are sequestered in the haemolymph as a defence against diurnal vertebrate predators and advertized by the usually aposematic coloration. An origin of the two Australian tribes from a common ancestor with the Poekilocerini is hypothesized for the Cretaceous period, throughout most of which Western Australia and India were in contact or near-contact. A 'lenticular organ' observed on the antennae of all the species has been found to occur widely in the Acridoidea.