gebhart/patrepeval huggingface dataset:
The <i>pilE</i> gene product of <i>Pseudomonas aeruginosa</i>, required for pilus biogenesis, shares amino acid sequence identity with the <i>N</i>‐termini of type 4 prepilin proteins (1994)

A new locus required for type 4 pilus biogenesis by Pseudomonas aeruginosa has been identified. A pilE mutant, designated MJ-6, was broadly resistant to pili-specific phages and unable to translocate across solid surfaces by the pilus-dependent mechanism of twitching motility (Twt-). Immunoblot analysis demonstrated that MJ-6 was devoid of pili (Pil-) but was unaffected in the production of unassembled pilin pools. Genetic studies aimed at localizing the pilE mutation on the P. aeruginosa PAO chromosome demonstrated a strong co-linkage between MJ-6 phage resistance and the proB marker located at 71 min. Cloning of the pilE gene was facilitated by the isolation and identification of a pro(B+)-containing plasmid from a PAO1 cosmid library. Upon introduction of the PAO1 proB+ cosmid clone into MJ-6, sensitivity to pili-specific phage, twitching motility and pilus production were restored. The nucleotide sequence of a 1 kb EcoRV-ClaI fragment containing the pilE region revealed a single complete open reading frame with characteristic P. aeruginosa codon bias. PilE, a protein with a molecular weight of 15,278, showed significant sequence identity to the pilin precursors of P. aeruginosa and to other type 4 prepilin proteins. The region of highest homology was localized to the N-terminal 40 amino acid residues. The putative PilE N-terminus contained a seven-residue basic leader sequence followed by a consensus cleavage site for prepilin peptidase and a largely hydrophobic region which contained tyrosine residues (Tyr-24 and Tyr-27) previously implicated in maintaining pilin subunit-subunit interactions. The requirement of PilE in pilus biogenesis was confirmed by demonstrating that chromosomal pilE insertion mutants were pilus- and twitching-motility deficient.
Human-Computer Interaction (2003)

This four volume set provides the complete proceedings of the 10th International Conference on Human-Computer Interaction held June, 2003 in Crete, Greece. A total of 2,986 individuals from industry, academia, research institutes, and governmental agencies from 59 countries submitted their work for presentation at the conference. The papers address the latest research and development efforts, as well as highlight the human aspects of design and use of computing systems. Those accepted for presentation thoroughly cover the entire field of human-computer interaction, including the cognitive, social, ergonomic, and health aspects of work with computers. The papers also address major advances in knowledge and effective use of computers in a variety of diversified application areas, including offices, financial institutions, manufacturing, electronic publishing, construction, health care, and disabled and elderly people.
Nonlinear Dynamics of the Phonon Stimulated Emission in Microwave Solid-State Resonator of the Nonautonomous Phaser Generator (2007)

The microwave phonon stimulated emission (SE) has been experimentally and numerically investigated in a nonautonomous microwave acoustic quantum generator, called also microwave phonon laser or phaser (see previous works arXiv:cond-mat/0303188 ; arXiv:cond-mat/0402640 ; arXiv:nlin.CG/0703050) Phenomena of branching and long-time refractority (absence of the reaction on the external pulses) for deterministic chaotic and regular processes of SE were observed in experiments with various levels of electromagnetic pumping. At the pumping level growth, the clearly depined increasing of the number of coexisting SE states has been observed both in real physical experiments and in computer simulations. This confirms the analytical estimations of the branching density in the phase space. The nature of the refractority of SE pulses is closely connected with the pointed branching and reflects the crises of strange attractors, i.e. their collisions with unstable periodic components of the higher branches of SE states in the nonautonomous microwave phonon laser.
Toll-like receptor 4 on stromal and hematopoietic cells mediates innate resistance to uropathogenic <i>Escherichia</i> <i>coli</i> (2003)

Innate host defenses at mucosal surfaces are critical in the early stages of many bacterial infections. In addition to cells of the traditional innate immune system, epithelial cells can also produce inflammatory mediators during an infection. However, the role of the epithelium in innate host defense in vivo is unclear. Recent studies have shown that lipopolysaccharide (LPS) recognition is critical for bladder epithelial cells to recognize and respond to Escherichia coli. Moreover, the LPS-nonresponsive mouse strain C3HHeJ, which has a mutation in the primary LPS receptor, Toll-like receptor 4 (TLR4), is extremely susceptible to infection with uropathogenic strains of E. coli. In this study, a bone marrow transplant approach was used to investigate the specific contributions of the bladder epithelium (and other stromal cells) in the TLR4-mediated innate immune response to the invading E. coli pathogen. Mice expressing the mutant TLR4 in the epithelialstromal compartment were not able to mount a protective inflammatory response to control the early infection even when their hematopoietic cells expressed wild-type TLR4. However, the presence of TLR4(+) epithelialstromal cells was not sufficient to activate an acute inflammatory response unless the hematopoietic cells were also TLR4(+). These results demonstrated that bladder epithelial cells play a critical role in TLR4-mediated innate immunity in vivo during a mucosal bacterial infection.
Smart Distributed Generation Systems Using Artificial Neural Network-Based Event Classification (2018)

Distributed generation (DG) sources have become an integral part of today's decentralized power systems. However, current DG systems are mostly passive and do not provide intelligent information to help detect power quality issues. In this paper, a novel and intelligent event classification scheme is proposed to provide the DG systems with real-time decision making capabilities. The proposed technique has the ability to provide information to help maintain the quality and reliability of the DG systems under various disturbances or operating conditions. This event classification technique was developed using artificial neural networks (ANNs) with a pre-defined set of local input parameters. The algorithm is implemented using four parallel ANNs that were designed to operate under a majority vote fusion algorithm representing the final classification output. A total of 310 event cases were generated to test the performance of the proposed technique. Simulation results showed that events were accurately classified within 10 cycles of their occurrences while achieving a 96.21% average classification accuracy.
Daylight visible/NIR whole-sky imagers for cloud and radiance monitoring in support of UV research programs (2003)

Measurements of UV radiation at the earth’s surface may be highly impacted by the presence of clouds. In order to provide support for UV research, a Daylight Visible/NIR Whole Sky Imager was developed to provide cloud fraction assessment over the whole sky, as well as measurements of the radiance distribution over the full sky in several spectral bands. Radiances are determined in approximately 700,000 directions simultaneously with a given optical filter. Data may be acquired in seven spectral bands that may be selected for the application. The current instrument uses filters near 450 and 650 nm, open-hole, filters in the blue-green broadband and NIR long-pass, and two polarizers. Opaque and thin cloud fraction is determined from images acquired in the blue and red wavelengths. A more sophisticated version of the algorithm to detect thinner clouds and enable aerosol assessment is in development, and will be based on use of the NIR data in conjunction with the blue and red data. This paper will provide an overview of the instrument design and calibration, and sample sky radiance results. The cloud algorithms for determination of cloud fraction will be discussed, and the cloud imager results will also be presented.
Interpretación de la función de onda—¿qué son los electrones? y ¿cómo se mueven? (2013)

En la mecánica cuántica, el estado físico de un electrón es descrito por una función de onda. Según la interpretación de probabilidad estándar, la función de onda de un electrón es amplitud de probabilidad, y su modulo cuadrado da la densidad de probabilidad de encontrar el electrón en una cierta posición en el espacio. En este artículo, se muestra que esta suposición central de la mecánica cuántica puede tener una extensión ontológica. Se argumenta que las partículas microscópicas como los electrones son realmente partículas, pero su movimiento no es continuo, sino discontinuo y aleatorio. Desde esta perspectiva, el modulo cuadrado de la función de onda no sólo da la densidad de probabilidad de que las partículas&nbsp;se encuentren&nbsp;en ciertos lugares, sino que también da la densidad de probabilidad de que las partículas&nbsp;estén&nbsp;allí. En otras palabras, la función de onda en la mecánica cuántica se puede considerar como una representación del estado de movimiento discontinuo aleatorio de las partículas, y en un nivel más profundo, puede representar la propiedad disposicional de las partículas que determina su movimiento discontinuo aleatorio.
The Proto-oncogene c-myc Acts through the Cyclin-dependent Kinase (Cdk) Inhibitor p27Kip1to Facilitate the Activation of Cdk4/6 and Early G1Phase Progression (2002)

Progression through the early G(1) phase of the cell cycle requires mitogenic stimulation, which ultimately leads to the activation of cyclin-dependent kinases 4 and 6 (Cdk4/6). Cdk4/6 activity is promoted by D-type cyclins and opposed by Cdk inhibitor proteins. Loss of c-myc proto-oncogene function results in a defect in the activation of Cdk4/6. c-myc(-/-) cells express elevated levels of the Cdk inhibitor p27(Kip1) and reduced levels of Cdk7, the catalytic subunit of Cdk-activating kinase. We show here that in normal (c-myc(+/+)) cells, the majority of cyclin D-Cdk4/6 complexes are assembled with p27 and remain inactive during cell cycle progression; their function is presumably to sequester p27 from Cdk2 complexes. A small fraction of Cdk4/6 protein was found in lower molecular mass catalytically active complexes. Conditional overexpression of p27 in c-myc(+/+) cells caused inhibition of Cdk4/6 activity and elicited defects in G(0)-to-S phase progression very similar to those seen in c-myc(-/-) cells. Overexpression of cyclin D1 in c-myc(-/-) cells rescued the defect in Cdk4/6 activity, indicating that the limiting factor is the number of cyclin D-Cdk4/6 complexes. Cdk-activating kinase did not rescue Cdk4/6 activity. We propose that the defect in Cdk4/6 activity in c-myc(-/-) cells is caused by the elevated levels of p27, which convert the low abundance activable cyclin D-Cdk4/6 complexes into unactivable complexes containing higher stoichiometries of p27. These observations establish p27 as a physiologically relevant regulator of cyclin D-Cdk4/6 activity as well as mechanistically a target of c-Myc action and provide a model by which c-Myc influences the early-to-mid G(1) phase transition.
Vinblastine-Loaded Nanoparticles with Enhanced Tumor-Targeting Efficiency and Decreasing Toxicity: Developed by One-Step Molecular Imprinting Process (2019)

Molecularly imprinted polymers have exhibited good performance as carriers on drug loading and sustained release. In this paper, vinblastine (VBL)-loaded polymeric nanoparticles (VBL-NPs) were prepared by a one-step molecular imprinting process, avoiding the waste and incomplete removal of the template, and evaluated as targeting carriers for VBL delivery after modification. Using acryloyl amino acid comonomers and disulfide cross-linkers, VBL-NPs were synthesized and then conjugated with poly(ethylene glycol)-folate. The dynamic size of the obtained VBL-NPs-PEG-FA was 258.3 nm (PDI = 0.250), and the encapsulation efficiency was 45.82 ± 1.45%. The nanoparticles of VBL-NPs-PEG-FA were able to completely release VBL during 48 h under a mimic tumor intracellular condition (pH 4.5, 10 mM glutathione (GSH)), displaying significant redox responsiveness, whereas the release rates were much slower in the mimic body liquid (pH 7.4, 2 μM GSH) and tumor extracellular environment (pH 6.5, 2 μM GSH). Furthermore, the carriers NPs-PEG-FA, prepared without VBL, showed satisfactory intrinsic hemocompatibility, cellular compatibility, and tumor-targeting properties: they could rapidly and efficiently accumulate to folate receptor positive Hela cells and then internalized via receptor-mediated endocytosis, and the retention in tumor tissues could last for over 48 h. Interestingly, VBL-NPs-PEG-FA could evidently increase the accumulation of VBL in tumor tissues while decreasing the distribution of VBL in organs, exert similar anticancer efficacy against Hela tumors in the xenograft model of nude mice to VBL injection, and significantly improve the abnormality of liver and spleen observed in VBL injection. VBL-NPs-PEG-FA has the potential to be the delivery carrier for VBL by enhancing the tumor-targeting efficacy of VBL and decreasing toxicity to normal tissues.
The Measurement of Performance of Combustors Using Passive Acoustic Methods (2004)

This paper presents results obtained as part of an ongoing proof of concept program being carried out by the University of Nevada, Reno and the NASA Glenn Research Center. The goal of this program is to develop passive acoustic methods which can be applied to determine the performance of jet engines and/or turbines. Six (6) acoustic transducers were mounted around a ring on a High Pressure Burner Rig (HPBR) located at the NASA Glenn Research Center. Data were recorded using a multi-track Sony digital data recorder. The HPBR was operated over several hours using a prescribed duty cycle of different fuel/air mixtures. Several signal processing methods were applied to this data including time-frequency and correlation techniques. Some very interesting results were obtained and are presented in this paper including the ability to determine the fuel/air mixtures on a continuing basis using only passive acoustic signature analysis methods.
Performance comparison between semicontinuous and discrete hidden Markov models of speech (1988)

The semicontinuous hidden Markov model (SCHMM) of speech has been shown to offer improved performance in comparison to conventional discrete HMM. Experimental results with different vector quantisation levels, different probability density functions, and different numbers of tokens in the training set for the SCHMM are reported here to compare with conventional discrete HMM.
The growth and structure of semiconducting thin films (1974)

In this review silicon and gallium arsenide are considered as typical examples of elemental and compound semiconductors respectively, and an account is given of the nucleation, growth and structure, both crystallographic and electrical, of autoepitaxial films of these materials. Some discussion of preparative techniques is first provided to illustrate the basic principles of thin film growth, but experimental details are not included. The use of modern methods of surface physics to assess the influence of the substrate surface on nucleation and subsequent growth behaviour is discussed, particularly with reference to results obtained using low energy electron diffraction. Auger electron spectroscopy and molecular beam techniques. Finally, the way in which studies of autoepitaxial growth have been extended to include growth on other substrates is considered.
IK of rabbit ventricle is composed of two currents: evidence for IKs (1996)

The delayed rectifier K+ current (IK) in rabbit heart has long been thought to consist of only a single, rapidly activating, dofetilide-sensitive current, IKr. However, we find that IK of rabbit ventricular myocytes actually consists of both rapid and slow components, IKr and IKs, respectively, that can be isolated pharmacologically. Thus, after complete blockade of IKr with dofetilide, the remaining current, IKs, is homogeneous as judged by an envelope of tails test. IKs activates and deactivates slowly, continues to activate during sustained depolarizations, has a half-activation potential of 7.0 +/- 0.8 mV and slope factor of 11.0 +/- 0.7 mV, reverses at -77.2 +/- 1.3 mV (extracellular K+ concentration = 4 mM), is increased by removing extracellular K+, and is enhanced by isoproterenol and stocked by azimilide. Northern analysis demonstrates that the minK (IsK) gene, which encodes a subunit of the channel that underlies the IKs current, is expressed in rabbit heart. Expression of the rabbit protein in Xenopus oocytes elicits a slowly activating, voltage-dependent current, IsK, similar to those expressed previously from mouse, rat, guinea pig, and human genes. The results demonstrate that IKs is present in rabbit ventricle and therefore contributes to cardiac repolarization in this species.
Single-wafer cluster tool performance: an analysis of throughput (1994)

Cluster tools gained greater acceptance over the past several years, although concerns still exist over the throughput these tools can achieve. This paper presents an analysis of the relationship between process times, transport times, and maximum throughput in an individual cluster tool. Theoretical models which quantify the time required to process both an individual wafer and a lot in a cluster tool are developed. Three techniques for increasing throughput, based on these models, are also presented. These modifications require minimal modification of many existing designs and can yield significant increases in performance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Cloning of the glucokinase gene in Escherichia coli B (1983)

A gene for glucokinase (Glk) in Escherichia coli B was cloned onto vector plasmid pBR322, and the hybrid plasmid obtained was designated pGK100. The gene for Glk was located in the central MluI fragment (0.82 megadalton) of the 6.0-megadalton chromosomal DNA inserted in the HindIII site of the vector. The introduction of pGK100 into E. coli 112L having a decreased level of Glk activity resulted in the about 15-fold increase in this enzyme activity. The poor growth rate of 112L cells on glucose or mannose was also improved by the introduction of pGK100. However, removal of some portion near the glk gene prevented the growth of 112L cells, although Glk activity was high enough to support growth. Therefore, some function of Glk may be regulated by a gene(s) near the glk gene.
The Reaction between Oxygen and 2-Butene (1935)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Reaction between Oxygen and 2-ButeneH. J. Lucas, A. N. Prater, and R. E. MorrisCite this: J. Am. Chem. Soc. 1935, 57, 4, 723–727Publication Date (Print):April 1, 1935Publication History Published online1 May 2002Published inissue 1 April 1935https://pubs.acs.org/doi/10.1021/ja01307a039https://doi.org/10.1021/ja01307a039research-articleACS PublicationsRequest reuse permissionsArticle Views182Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evidence for a cytopathogenicity determinant in HIV-1 Vpr (2002)

HIV-1 is cytopathic for CD4(+) T lymphocytes in vitro and this property of HIV-1 is generally considered to account for some of its in vivo cytopathogenicity. Thus, the extent of lymphocyte depletion correlates with the level of viremia whereas low levels of viral replication are typically associated with stable lymphocyte levels and asymptomatic infection such as is observed in non-progressors. Here, we describe a non-progressor who did not fit this general pattern in that CD4(+) T lymphocyte homeostasis was maintained in the face of high-level viral replication. Biological viral isolates from this patient replicated in primary lymphocytes without inducing cytopathicity. Because this phenotype is reminiscent of Vpr-deleted viruses, we examined the contribution of the Vpr gene to the viral phenotype. Vpr alleles derived from this patient contained both premature stop codons and an unusual Q3R polymorphism. Insertion of patient-derived Vpr alleles or a Q3R substitution into a cytopathic HIV-1 clone resulted in a marked impairment of cytopathicity without affecting viral replication efficiency. The effect of Vpr on cytopathicity was unrelated to reported activities of Vpr including virion association, interaction with uracil DNA glycosylase, G(2) arrest, or enhancement of macrophage infection but correlated with the ability of Vpr to induce host cell apoptosis. This study suggests the presence of a determinant of in vivo cytopathogenicity within HIV-1 Vpr and further indicates that viral replication can be uncoupled from cytopathicity in vitro and in vivo.
Co-Exchanged IM5, a Stable Zeolite for the Selective Catalytic Reduction of NO in the Presence of Water and SO<sub>2</sub> (2003)

IM5 is a recently discovered zeolite that presents a high hydrothermal stability and, when exchanged with cobalt, shows a high activity for the selective catalytic reduction of NO with propane. The catalytic behavior of a cobalt-exchanged IM5 zeolite is compared with that of a cobalt-exchanged ZSM5 zeolite. The state of the cobalt in the zeolite and the influence of the partial pressure of oxygen, reaction temperature, and the presence of water and SO2 are studied. It is shown that cobalt is mainly as Co2+ in a tetrahedral environment, although the presence of a small amount of other cobalt states cannot be rejected. The material is very active for the selective catalytic reduction of NOx with propane at 723 K and, what is important, with a high oxygen concentration. The presence of possible catalyst poisons such as SO2 and water does not result in a deactivation of the Co−IM5.
Multifunctional Homopolymers: Postpolymerization Modification via Sequential Nucleophilic Aromatic Substitution (2016)

Despite recent progress for efficient and precise synthesis of functional polymers, few reports describe postpolymerization functionalization strategies for the controlled incorporation of multiple pendent groups per repeat unit. Cyanuric chloride, or 2,4,6-trichloro-1,3,5-triazine (TCT), offers a facile method to introduce distinct pendent functionalities. An acrylamide monomer containing a triazine ring with two electrophilic sites was prepared from TCT and polymerized via reversible addition–fragmentation chain transfer (RAFT) polymerization. Subsequent nucleophilic aromatic substitution reactions using a combination of amine and thiol nucleophiles introduced two functionalities into each repeat unit of the RAFT-derived polymers. The success of each postpolymerization modification reaction was confirmed by 1H NMR spectroscopy and size-exclusion chromatography, and small molecule model studies corroborated the high chemoselectivity of the nucleophilic aromatic substitution reactions. This postpolymerization modification strategy based on TCT enables a facile and efficient synthesis of multifunctional homopolymers.