gebhart/patrepeval huggingface dataset:
Immune-evasive gene switch enables regulated delivery of chondroitinase after spinal cord injury (2018)

Chondroitinase ABC is a promising preclinical therapy that promotes functional neuroplasticity after CNS injury by degrading extracellular matrix inhibitors. Efficient delivery of chondroitinase ABC to the injured mammalian spinal cord can be achieved by viral vector transgene delivery. This approach dramatically modulates injury pathology and restores sensorimotor functions. However, clinical development of this therapy is limited by a lack of ability to exert control over chondroitinase gene expression. Prior experimental gene regulation platforms are likely to be incompatible with the non-resolving adaptive immune response known to occur following spinal cord injury. Therefore, here we apply a novel immune-evasive dual vector system, in which the chondroitinase gene is under a doxycycline inducible regulatory switch, utilizing a chimeric transactivator designed to evade T cell recognition. Using this novel vector system, we demonstrate tight temporal control of chondroitinase ABC gene expression, effectively removing treatment upon removal of doxycycline. This enables a comparison of short and long-term gene therapy paradigms in the treatment of clinically-relevant cervical level contusion injuries in adult rats. We reveal that transient treatment (2.5 weeks) is sufficient to promote improvement in sensory axon conduction and ladder walking performance. However, in tasks requiring skilled reaching and grasping, only long term treatment (8 weeks) leads to significantly improved function, with rats able to accurately grasp and retrieve sugar pellets. The late emergence of skilled hand function indicates enhanced neuroplasticity and connectivity and correlates with increased density of vGlut1+ innervation in spinal cord grey matter, particularly in lamina III-IV above and below the injury. Thus, our novel gene therapy system provides an experimental tool to study temporal effects of extracellular matrix digestion as well as an encouraging step towards generating a safer chondroitinase gene therapy strategy, longer term administration of which increases neuroplasticity and recovery of descending motor control. This preclinical study could have a significant impact for tetraplegic individuals, for whom recovery of hand function is an important determinant of independence, and supports the ongoing development of chondroitinase gene therapy towards clinical application for the treatment of spinal cord injury.
Software Maintenance Maturity Model (SMmm): the software maintenance process model (2005)

We address the assessment and improvement of the software maintenance function by proposing a maturity model for daily software maintenance activities: the Software Maintenance Maturity Model (SMmm). The software maintenance function suffers from a scarcity of management models to facilitate its evaluation, management, and continuous improvement. The SMmm addresses the unique activities of software maintenance while preserving a structure similar to that of the Capability Maturity Model integration (CMMi). It is designed to be used as a complement to that model. The SMmm is based on practitioners' experience, international standards, and the seminal literature on software maintenance. We present the model's purpose, scope, foundation, and architecture, followed by its initial validation. Copyright © 2005 John Wiley & Sons, Ltd.
Physical Activity/Exercise and Diabetes: A Position Statement of the American Diabetes Association (2016)

The adoption and maintenance of physical activity are critical foci for blood glucose management and overall health in individuals with diabetes and prediabetes. Recom- mendations and precautions vary depending on individual characteristics and health status. In this Position Statement, we provide a clinically oriented review and evidence- based recommendations regarding physical activity and exercise in people with type 1 diabetes, type 2 diabetes, gestational diabetes mellitus, and prediabetes. Physical activity includes all movement that increases energy use, whereas exer- cise is planned, structured physical activity. Exercise improves blood glucose control in type 2 diabetes, reduces cardiovascular risk factors, contributes to weight loss, and improves well-being (1,2). Regular exercise may prevent or delay type 2 di- abetes development (3). Regular exercise also has considerable health bene fi ts for people with type 1 diabetes (e.g., improved cardiovascular fi tness, muscle strength, insulin sensitivity, etc.) (4). The challenges related to blood glucose management vary with diabetes type, activity type, and presence of diabetes-related complica- tions (5,6). Physical activity and exercise recommendations, therefore, should be tailored to meet the speci fi c needs of each individual.
Na++K+-ATPase in gills of the blue crab<i>Callinectes sapidus</i>: cDNA sequencing and salinity-related expression of α-subunit mRNA and protein (2001)

Many studies have shown that hyperosmoregulation in euryhaline crabs is accompanied by enhanced Na(+)+K(+)-ATPase activity in the posterior gills, but it remains unclear whether the response is due to regulation of pre-existing enzyme or to increased gene transcription and mRNA translation. To address this question, the complete open reading frame and 3' and 5' untranslated regions of the mRNA coding for the alpha-subunit of Na(+)+K(+)-ATPase from the blue crab Callinectes sapidus were amplified by reverse transcriptase/polymerase chain reaction (RT-PCR) and sequenced. The resulting 3828-nucleotide cDNA encodes a putative 1039-amino-acid protein with a predicted molecular mass of 115.6 kDa. Hydrophobicity analysis of the amino acid sequence indicated eight membrane-spanning regions, in agreement with previously suggested topologies. The alpha-subunit amino acid sequence is highly conserved among species, with the blue crab sequence showing 81-83 % identity to those of other arthropods and 74-77 % identity to those of vertebrate species. Quantitative RT-PCR analysis showed high levels of alpha-subunit mRNA in posterior gills 6-8 compared with anterior gills 3-5. Western blots of gill plasma membranes revealed a single Na(+)+K(+)-ATPase alpha-subunit protein band of the expected size. The posterior gills contained a much higher level of alpha-subunit protein than the anterior gills, in agreement with previous measurements of enzyme activity. Immunocytochemical analysis showed that the Na(+)+K(+)-ATPase alpha-subunit protein detected by alpha5 antibody is localized to the basolateral membrane region of gill epithelial cells. Transfer of blue crabs from 35 to 5 per thousand salinity was not accompanied by notable differences in the relative proportions of alpha-subunit mRNA and protein in the posterior gills, suggesting that the enhanced Na(+)+K(+)-ATPase enzyme activity that accompanies the hyperosmoregulatory response may result from post-translational regulatory processes.
DNS ontology-based search service (2006)

In this paper, we design and implement a DNS ontology-based, three-layer (i.e., presentation layer, logic layer and data layer) semantic search service. The primary goal is to strengthen the sharing and reuse of DNS knowledge through the iDNS-KDE knowledge portal (http://idns-kde.nctu.edu.tw) to facilitate people in locating the required information and to help alleviate the system management issues on the DNS domain. We adopt the DNS ontology as the semantics resolution mechanism to improve the search capability. It is used for enhancing the semantic search and extending the original diagnosis-and-tutoring model in iDNS-KDE to become the ”diagnosis-search-tutoring” model. On the other hand, the separation of the layers could make the whole system more flexible and reusable.
Comparison of rat and rat-mouse chimeric anti-murine CD4 antibodies in vitro. Chimeric antibodies lyse low-density CD4+ cells. (1989)

GK1.5, a rat anti-mouse CD4 mAb, is effective in the treatment of several autoimmune syndromes, induces tolerance to co-administered Ag, and prolongs allograft survival. We have constructed a family of molecules with GK1.5 V regions and mouse gamma 1, gamma 2a, gamma 2b, or gamma 3 constant regions to investigate the mechanisms underlying the effectiveness of GK1.5. The rat-mouse chimeric antibodies are specific for murine CD4 and have identical binding curves as rat GK1.5 on CD4+ T cells. The chimeric GK1.5 gamma 2a, GK1.5 gamma 2b, and GK1.5 gamma 3 antibodies are more efficient than rat GK1.5 at C-mediated cytotoxicity. This is attributed to the enhanced capacity of the chimeric antibodies, compared to rat GK1.5, to lyse CD4+ cells with a low cell surface Ag density. This observation may have important implications for therapy.
Hot-carrier transport processes in stimulated desorption of alkali halides (1998)

Electron- and photon-stimulated desorption of epitaxial thin films and bulk single crystals of alkali halides has been investigated. It has been found that the widely accepted model based on self-trapped exiton decay and thermally activated defect diffusion cannot account for a number of experimental observations. In particular, it cannot explain the stability of the very thin alkali-halide films against the electron beam, and it fails to interpret correctly the yield dependence of the halogen atoms emitted with nonthermal energies on the film thickness and sample temperature. It is shown that for satisfactory interpretation of those data one has to take into account early stages of crystal excitation, i.e., hot carrier formation and transport processes occurring prior to self-trapped exciton phase. Consequently, a comprehensive description of the observed desorption features is presented.
Modulation of collagen synthesis in normal and osteoarthritic cartilage (2004)

In osteoarthritic cartilage, chondrocytes are able to present heterogeneous cellular reactions with expression and synthesis of the (pro)collagen types characteristic of prechondrocytes (type IIA), hypertrophic chondrocytes (type X), as well as differentiated (types IIB, IX, XI, VI) and dedifferentiated (types I, III) chondrocytes. The expression of type IIA procollagen in human osteoarthritic cartilage support the assumption that OA chondrocytes reverse their phenotype towards a chondroprogenitor phenotype. Recently, we have shown that dedifferentiation of mouse chondrocytes induced by subculture was associated with the alternative splicing of type II procollagen pre-mRNA with a switch from the IIB to the IIA form. In this context, we demonstrated that BMP-2 favours expression of type IIB whereas TGF-beta1 potentiates expression of type IIA induced by subculture. These data reveal the specific capability of BMP-2 to reverse the program of chondrocyte dedifferentiation. This interesting feature needs to be tested with human chondrocytes since cell amplification is required for the currently used autologous chondrocyte transplantation.
Varicoceles: a coaxial coil occlusion system. (1984)

A modified transfemoral technique for testicular venography and occlusion of the testicular vein (TV) was used to treat 30 patients with a total of 43 varicoceles (17 left-sided and 13 bilateral). Occlusion was satisfactory in all left varicoceles and 90% of right varicoceles. This coaxial technique facilitates subselective catheterization of the TV and its collaterals and enables coils to be placed deep within these vessels, either alone or in combination with detachable balloons.
Addition of Nucleotides Similar to Deleted CAA Repeats in the 5' Non-coding Region of Tomato Mosaic Virus RNA Following Propagation (1996)

Previously we made a series of deletion mutants in the 5' non-coding region of tomato mosaic tobamovirus (ToMV) RNA and checked their ability to replicate in tobacco protoplasts. Long deletions in this region caused the virus to lose the ability to replicate. Several mutants with deletions of about 10 nucleotides (short deletion mutants; SDM) retained the ability to replicate. In this study, we inoculated SDMs onto systemic host plants and observed their symptoms. One mutant (19/32) caused severe mosaic symptoms on some tobacco plants (Nicotiana tabacum cv. Samsun) but no symptoms on others. Virus accumulation in 19/32-inoculated plants paralleled the severity of symptoms. Four CAA repeat sequences were deleted in 19/32. Progeny 19/32 from plants showing severe systemic mosaic symptoms had acquired additional nucleotides in this region. We conclude that the CAA repeat sequence is related to the fitness of the virus population to replicate in whole plants rather than to translation of ToMV replicase genes.
Fault diagnosis of plasma etch equipment (2002)

The development and implementation of robust methods for fault detection promises to enhance manufacturing by improving our capability to monitor equipment and processes. In order to fully utilize this capability it is important that the machine fault is not only detected, but also diagnosed as belonging to a fault category so that appropriate corrective action can promptly be taken. In this paper we examine the diagnostic performance of two probabilistic modeling techniques in using sensor signals to classify faults. We also discuss how the strengths of these models may be combined in a hierarchical architecture giving rise to a more powerful diagnostic tool.
The Effect of Urapidil on Neurogenic Bladder: A Placebo Controlled Double-Blind Study (1996)

Urapidil improved voiding dysfunction in patients with a neurogenic bladder and decreased urethral resistance in a dose related fashion.
Imaginal discs can be recovered from cultured embryos mutant for the segment-polarity genes <i>engrailed, naked</i> and <i>patched</i> but not from <i>wingless</i> (1989)

Drosophila embryos homozygous for strong mutations in each of the segment-polarity genes wingless (wg), engrailed (en), naked (nkd) and patched (ptc) form a larval cuticle in which there is a deletion in every segment. The mutant embryos normally fail to hatch but by in vivo culture we were able to show which could produce adult structures. Cultured wg⁻ embryos did not produce any adult structures. Cultured en⁻ embryos produced eye-antennal derivatives and rarely produced partial thoracic structures. nkd⁻ and ptc⁻ embryos produced eye-antennal and thoracic derivatives. The nkd⁻ and ptc⁻ thoracic imaginal discs developed with an abnormal morphology and abnormal pattern of en-expression. Our findings are consistent with the idea that the thoracic imaginal discs derive from two adjacent groups of cells that express wg and en respectively in the embryo.
Physiology of the pancreatic α-cell and glucagon secretion: role in glucose homeostasis and diabetes (2008)

The secretion of glucagon by pancreatic alpha-cells plays a critical role in the regulation of glycaemia. This hormone counteracts hypoglycaemia and opposes insulin actions by stimulating hepatic glucose synthesis and mobilization, thereby increasing blood glucose concentrations. During the last decade, knowledge of alpha-cell physiology has greatly improved, especially concerning molecular and cellular mechanisms. In this review, we have addressed recent findings on alpha-cell physiology and the regulation of ion channels, electrical activity, calcium signals and glucagon release. Our focus in this review has been the multiple control levels that modulate glucagon secretion from glucose and nutrients to paracrine and neural inputs. Additionally, we have described the glucagon actions on glycaemia and energy metabolism, and discussed their involvement in the pathophysiology of diabetes. Finally, some of the present approaches for diabetes therapy related to alpha-cell function are also discussed in this review. A better understanding of the alpha-cell physiology is necessary for an integral comprehension of the regulation of glucose homeostasis and the development of diabetes.
Endometrial and Pituitary Responses to the Steroidal Antiprogestin RU 486in Postmenopausal Women (1985)

The effects of the antiprogestin RU 486 on the human endometrium were investigated. Seventeen postmenopausal women were injected with estradiol (E2) benzoate (0.625 mg/day) for 15 days. Progesterone (P) (25 mg/day) and/or RU 486 (100 or 200 mg/day) were given to groups of 2-3 women during the last 6 days of E2 benzoate treatment. Serial blood samples were drawn for the measurement of plasma E2, P, and LH and FSH. An endometrial biopsy was performed on the last day of treatment, and processed for histology or for assays of DNA polymerase alpha, E2-dehydrogenase (E2DH), and P receptor (PR). Treatment with E2 benzoate alone resulted in a marked decrease of plasma gonadotropins; in those patients who received either P, RU 486, or both, in addition to E2 benzoate, the concentrations of plasma LH and FSH were further decreased to premenopausal levels. In absence of glycerol, the affinity of RU 486 for the endometrial PR (Kd = 0.8 nM) was higher than that of P (Kd = 1.2 nM). Glycerol decreased markedly the affinity of RU 486, whereas the affinity of P for the PR was unchanged. RU 486 had negligible affinity for plasma transcortin. Either P or RU 486, but not both together, induced secretory changes in the endometrium as determined from histologic sections of tissue biopsies. Either P or RU 486 decreased DNA polymerase alpha and increased E2-DH activities in the endometrium. Unexpectedly, when P and RU 486 were given together. E2-DH activity remained at the level found in E2-treated women. In vitro cultures of proliferative endometrium treated with the synthetic progestagen R 5020 or with RU 486 also had increased E2-DH activity; RU 486 counteracted R 5020 effects. We conclude that, contrary to previous results with experimental animals, the anti-P RU 486 has some progestomimetic activity in humans under specific conditions. Paradoxically, when given together with P, RU 486 lost most of its progestomimetic activity in the endometrium and behaved as a pure antagonist.
Imaginário e memória: interações sígnicas na arte amazônica contemporânea (2016)

O artigo propoe uma reflexao sobre trajetorias ecossistemicas comunicacionais, por meio de memorias e metamorfoses da arte. Tem o objetivo de compreender aspectos da interacao entre corpo, mente e ambiente, em processos artisticos no Amazonas. A abordagem e feita pelo vies da semiotica e o referencial teorico traz autores como Lucia Santaella, Christine Greiner e Paes Loureiro. E possivel perceber cartografias signicas construidas pelo imaginario, a partir de inter-relacoes e interdependencias.
Deformation Behavior of Metal Rubber Material (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Comparison of the Antiproliferative Activity of Two AntitumourRuthenium(III) Complexes With Their Apotransferrin andTransferrin‐Bound Forms in a Human Colon Cancer Cell Line (1995)

Two ruthenium(III) complexes, namely trans-indazolium[tetrachlorobis(indazole)- ruthenate(III)], HInd[RuInd(2)Cl(4)] and trans-imidazolium[tetrachlorobis(imidazole)- ruthenate(III)], HIm[RuIm(2)Cl(4)] exhibit high anticancer activity in an autochthonous colorectal carcinoma model in rats. Recently, it has been shown that both complexes bind specifically to human serum apotransferrin and the resulting adducts have been studied through spectroscopic and chromatographic techniques with the ultimate goal of preparing adducts with good selectivity for cancer cells due to the fact that tumour cells express high amounts of transferrin receptors on their cell surface.In order to investigate whether the cellular uptake of the complexes was mediated by apotransferrin or transferrin, we compared the antiproliferative efficacy of HInd[RuInd(2)Cl(4)] and HIm[RuIm(2)Cl(4)] with its apotransferrin- and transferrin-bound form in the human colon cancer cell line SW707 using the microculture tetrazolium test (MTT).Our results show that especially the transferrin-bound forms exhibit high antiproliferative activity, which exceeds that of the free complex, indicating that this protein can act as a carrier of the ruthenium complexes into the tumor cell.
Fast‐Rate Capable Electrode Material with Higher Energy Density than LiFePO<sub>4</sub>: 4.2V LiVPO<sub>4</sub>F Synthesized by Scalable Single‐Step Solid‐State Reaction (2015)

Use of compounds that contain fluorine (F) as electrode materials in lithium ion batteries has been considered, but synthesizing single-phase samples of these compounds is a difficult task. Here, it is demonstrated that a simple scalable single-step solid-state process with additional fluorine source can obtain highly pure LiVPO<sub>4</sub>F. The resulting material with submicron particles achieves very high rate capability ≈100 mAh g<sup>-1</sup> at 60 C-rate (1-min discharge) and even at 200 C-rate (18 s discharge). It retains superior capacity, ≈120 mAh g<sup>-1</sup> at 10 C charge/10 C discharge rate (6-min) for 500 cycles with >95% retention efficiency. Furthermore, LiVPO<sub>4</sub>F shows low polarization even at high rates leading to higher operating potential >3.45 V (≈3.6 V at 60 C-rate), so it achieves high energy density. It is demonstrated for the first time that highly pure LiVPO<sub>4</sub>F can achieve high power capability comparable to LiFePO<sub>4</sub> and much higher energy density (≈521 Wh g<sup>-1</sup> at 20 C-rate) than LiFePO<sub>4</sub> even without nanostructured particles. LiVPO<sub>4</sub>F can be a real substitute of LiFePO<sub>4.</sub>
Use of Steel Slag Leach Beds for the Treatment of Acid Mine Drainage: The McCarty Highwall Project (2002)

Steel slag leach beds were constructed at the abandoned McCarty mine site in Preston County, West Virginia. The leach beds were constructed as slag check dams below limestone-lined settling basins. Acid water was captured in limestone channels and directed into basins to leach through the slag dams and discharge into a tributary of Beaver Creek. Since installation in October 2000, the system has been consistently producing net alkaline, pH 9.0 water. The water is still net alkaline and has stabilized at a neutral pH after the treated water encounters several other acidic seeps downstream. In addition, other than elevated Cr, which exceeds EPA water criteria for freshwater aquatics at the Beaver pond discharge, metals in the system effluent are within acceptable limits.
BASIC PROPERTIES OF MAGNETIC SHAPE MEMORY ACTUATORS (2002)

Magnetically controlled shape memory materials are a new w ay to p roduce motion and force. The material changes s hape in a magnetic field. Shape c hanges are e ven l0 p ercent and response time is less than a millisecond. Usual applications of the material are ac tuators that produce linear motion. In this article several magnetic shape memory actuators are presented and their properties and advantages are discussed.
Protocol stack-based telecom emulator (2002)

The paper describes the concept and implementation of a telecom emulator that features both reconfigurability and high-speed processing. The emulator can be easily transmuted into any telecom system as a real node. It has two innovative system design concepts. The first is to divide the specification into simplified processes based on the open system interconnection (OSI) reference model. The second is the use of a sophisticated hardmacro and its software-callable driver. We implemented a prototype system called ATTRACTOR and applied it to some telecom applications. The applications were able to be implemented in a short design time and were operated in real computer network environments.