gebhart/patrepeval huggingface dataset:
Skeleton-Space: a Multiscale Shape Description Combining Region and Boundary Information (1994)

A multiscale extension to the medial axis transform(MAT) or skeleton can be obtained by combining information derived from a scale-space hierarchy of boundary representations with region information provided by the MAT. The skeleton-space is constructed by attributing each skeleton component with a hierarchically ordered sequence of residual values, each expressing the saliency of the component at a distinct resolution level. Since our method amounts to a rather symbolic than iconic computation of a multiscale MAT, it does not introduce the correspondence problem between distinct levels of detail, in contrast to other commonly proposed techniques. Our multiscale MAT is capable of describing complex shapes characterized by significantly jagged boundaries. Furthermore, tracking the evolutionof prominent loci of the MAT such as nodes across scales permits to assess the most significant skeleton constituents and to automatically determine pruning parameters. A salient subset of the MAT ...
The Gestalt two-chair technique: how it relates to theory (2009)

Using Q-Methodology, this study sought to find empirical support for the three stage model of the Gestalt two-chair technique and the theory underlying how and why it is effective, put forth by L. S. Greenberg (1979; 1983). A structured Q-Sort was constructed using the factors of Conflict Resolution (CR) and the Gestalt concept of Contact (C) in a 2X2 factorial design. Each factor was divided into two levels CR - Resolved/Unresolved, C - Interruption-of-contact/Contact. The factors of CR and C were expected to interact before and after successful and unsuccessful therapy for decision-making. Individuals who were Unresolved in their decision-making were expected to experience Interruption-of-contact while individuals who were Resolved from an integrated sense of self were expected to experience being in Contact. The 85 item sort was validated by experts in Gestalt theory and therapy as representing the three stage model and the theory underlying it. Eight subjects, ambivalent about remaining in their marriages, performed the Q-Sort before and after 6 sessions of therapy using the two-chair technique. Moderate support was found for the 3 stages of the model, Opposition, Merging and Integration. Some support was found for the interaction of the factors of CR and C. Significant 2-way interaction was found for 1 subject before and after therapy considered successful according to the model. Significant 2-way interactions were found after successful therapy for four subjects but not before. Significant 2-way interactions were not found for subjects after unsuccessful therapy. When therapy was successful the factors of CR and C interacted as predicted. When therapy was not successful the factors of CR and C did not interact as predicted. The factors of CR and C did not interact for individuals who were experiencing a great deal of interruption-of-contact, indicating there is a possible pre-stage to the model where CR and C are independent of each other or where other factors may be involved. This study expanded the research on the Gestalt two-chair technique in several ways. It found some empirical support for the model and the theory underlying it. It found areas were CR and C do not interact as predicted by theory. It extended the range of application of research on two-chair technique from career decision-making to divorce decision-making.
A versatile modular vector system for rapid combinatorial mammalian genetics (2015)

Here, we describe the multiple lentiviral expression (MuLE) system that allows multiple genetic alterations to be introduced simultaneously into mammalian cells. We created a toolbox of MuLE vectors that constitute a flexible, modular system for the rapid engineering of complex polycistronic lentiviruses, allowing combinatorial gene overexpression, gene knockdown, Cre-mediated gene deletion, or CRISPR/Cas9-mediated (where CRISPR indicates clustered regularly interspaced short palindromic repeats) gene mutation, together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging. Examples of tumor engineering were used to illustrate the speed and versatility of performing combinatorial genetics using the MuLE system. By transducing cultured primary mouse cells with single MuLE lentiviruses, we engineered tumors containing up to 5 different genetic alterations, identified genetic dependencies of molecularly defined tumors, conducted genetic interaction screens, and induced the simultaneous CRISPR/Cas9-mediated knockout of 3 tumor-suppressor genes. Intramuscular injection of MuLE viruses expressing oncogenic H-RasG12V together with combinations of knockdowns of the tumor suppressors cyclin-dependent kinase inhibitor 2A (Cdkn2a), transformation-related protein 53 (Trp53), and phosphatase and tensin homolog (Pten) allowed the generation of 3 murine sarcoma models, demonstrating that genetically defined autochthonous tumors can be rapidly generated and quantitatively monitored via direct injection of polycistronic MuLE lentiviruses into mouse tissues. Together, our results demonstrate that the MuLE system provides genetic power for the systematic investigation of the molecular mechanisms that underlie human diseases.
Laboratory and clinical studies of cancer chemoprevention by antioxidants in berries (2008)

Reactive oxygen species (ROS) are a major cause of cellular injury in an increasing number of diseases, including cancer. Most ROS are created in the cell through normal cellular metabolism. They can be produced by environmental insults such as ultraviolet light and toxic chemicals, as well as by the inflammatory process. Interception of ROS or limiting their cellular effects is a major role of antioxidants. Due to their content of phenolic and flavonoid compounds, berries exhibit high antioxidant potential, exceeding that of many other foodstuffs. Through their ability to scavenge ROS and reduce oxidative DNA damage, stimulate antioxidant enzymes, inhibit carcinogen-induced DNA adduct formation and enhance DNA repair, berry compounds have been shown to inhibit mutagenesis and cancer initiation. Berry constituents also influence cellular processes associated with cancer progression including signaling pathways associated with cell proliferation, differentiation, apoptosis and angiogenesis. This review article summarizes laboratory and human studies, demonstrating the protective effects of berries and berry constituents on oxidative and other cellular processes leading to cancer development.
Inter‐relationships between rheumatoid arthritis and periodontal disease (2003)

This review considers the considerable similarities between periodontal disease and rheumatoid arthritis (RA). While the etiology of these two diseases may differ, the underlying pathogenic mechanisms are remarkably similar and it is possible that individuals manifesting both periodontitis and RA may suffer from a unifying underlying systemic dysregulation of the inflammatory response. In light of these findings, the implications for the use of disease-modifying medications in the management of these two chronic inflammatory conditions is apparent. Further longitudinal studies and medication-based intervention studies are required to determine just how closely these two conditions are allied.
Generation of an erythromycin-sensitive derivative of Clostridium difficile strain 630 (630Δerm) and demonstration that the conjugative transposon Tn916ΔE enters the genome of this strain at multiple sites (2005)

Erythromycin resistance in Clostridium difficile strain 630 is conferred by a genetic element termed Tn5398 which contains two erm(B) genes: erm1(B) and erm2(B). An erythromycin-sensitive derivative of strain 630 (designated 630Deltaerm) was generated by spontaneous mutation after continuous subculture for 30 days. This strain had lost the erm2(B) gene from within Tn5398 but retained erm1(B). However, the strain could revert to erythromycin resistance at a frequency of 2.79 x 10(-8), although it still contained the deletion of erm2(B). The availability of C. difficile 630Deltaerm allowed the behaviour of Tn916DeltaE to be investigated in this strain. This element entered the genome at multiple sites indicating that it could be useful as an insertional mutagen.
Machine learning approaches for epitope prediction (2008)

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix CHAPTER 1. GENERAL INTRODUCTION . . . . . . . . . . . . . . . . . .
Timing Vulnerability Factors of Sequentials (2004)

Single-event upsets (SEU) from particle strikes have become a key challenge in microprocessor design. Modern superpipelined microprocessors typically contain many thousands of sequentials whose soft-error rate (SER) cannot be neglected any more. An accurate assessment of the SER of sequentials is therefore crucial. This work describes a method for computing timing vulnerability factors (TVFs) of sequentials. Our methology captures the impact of the circuit environment which sequentials are typically placed in. Further, upsets occurring in local clock nodes have been accounted for. Results are presented for master-slave type flip flops and for flow-through latches of a high-performance microprocessor. Our investigations demonstrate that TVFs are a strong function of the propagation delay of the combinational logic and typically vary between /spl sim/0% and 50%. For high-performance microprocessors, we predict average TVF values of the order of 20%-30%. Further, we expect TVFs to be largely technology independent for the same design.
Cutting Edge: Adenovirus E19 Has Two Mechanisms for Affecting Class I MHC Expression (1999)

Viral strategies for immune evasion include inhibition of various steps in the class I MHC assembly pathway. Here, we demonstrate that adenovirus produces one gene product with a dual function in this regard. It is well established that adenovirus E19 binds class I molecules and retains them in the endoplasmic reticulum (ER). However, E19 also delays the expression of class I alleles to which it cannot tightly bind. Here, we show that E19 binds TAP and acts as a tapasin inhibitor, preventing class I/TAP association. DeltaE19, an E19 mutant lacking the ER-retention signal, delays maturation of class I molecules, indicating that E19's inhibition of class I/TAP interaction is sufficient to delay class I expression. These data identify tapasin inhibition as a novel mechanism of viral immune evasion and suggest that, through this secondary mechanism, adenovirus can affect Ag presentation by MHC alleles that it can only weakly affect by direct retention.
The luminescent spectra of zinc sulphide and zinc cadmium sulphide phosphors (1946)

The first page of this article is displayed as the abstract.
Hierarchical lane detection for different types of roads (2008)

This paper presents a hierarchical lane detection system with the ability to deal with both structured and unstructured roads. The proposed system classifies the environment first before applying suitable algorithms for different types of roads. For environment classification, pixels with lane- marking colors are extracted as feature points. Eigenvalue decomposition regularized discriminant analysis is utilized in model selection and maximum likelihood estimation of Gaussian parameters in high dimensional feature space. For structured roads, the extracted feature points are reused for lane detection. For unstructured roads, mean-shift segmentation is applied to divide the scene into regions. Possible road boundary candidates are selected, and Bayes rule is used to choose the most probable boundary pairs. The experimental results show that the system is able to robustly find the boundaries of the lanes on different types of roads and various weather conditions.
Phase-coherent transport in hybrid superconducting nanostructures (1998)

This article is an overview of recent experimental and theoretical work on transport in phase-coherent hybrid nanostructures, with particular emphasis on dc electrical conduction. A summary of multiple scattering theory and the quasi-classical methods is presented and comparisons between the two are made. Several paradigms of phase-coherent transport are discussed, including zero-bias anomalies, reentrant and long range proximity effects, Andreev interferometers and superconductivity-induced conductance suppression.
GnRH-Deficient Phenotypes in Humans and Mice with Heterozygous Variants in<i>KISS1</i>/<i>Kiss1</i> (2011)

Deleterious, heterozygous RSV in KISS1 exist at a low frequency in GnRH-deficient patients as well as in the general population in presumably normal individuals. As in Kiss1(+/-)/Kiss1r(+/-) mice, heterozygous KISS1 variants in humans may work with other genetic and/or environmental factors to cause abnormal reproductive function.
Fabrication of high current and low profile micromachined inductor with laminated Ni/Fe core (2002)

A new process for the fabrication of high current and very low profile micromachined inductors has been developed. This process involves the combination of mechanical lamination and electrodeposition of copper windings by means of LIGA-like lithography through thick epoxy photoresists. The dimension of the fabricated inductor is 16 mm/spl times/19 mm/spl times/1 mm. The fabricated inductor has an inductance value of 1.2 /spl mu/H with DC saturation current of 3 A and an electrical resistance of less than 30 m/spl Omega/ at 10 kHz.
Gangliosides and Neurotrophic Growth Factors in the Retina: Molecular Interactions and Applications as Neuroprotective Agents<sup>a</sup> (1998)

Polypeptide growth factors and gangliosides can both be considered as trophic agents involved in almost all stages of neural cell development, differentiation, survival, and pathology. In most cases their physiological roles are still not clear due to the considerable complexity in their regulation. Several growth factors [e.g., basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF)] and one species of ganglioside (GM1) have been shown to exert interactions with each other and also to exhibit neuroprotective effects against retinal ischemia in vivo and cerebral excitotoxicity in vitro. Different experimental models are used to investigate their relevance to ischemic and excitotoxic conditions in the retina, and it is shown that (1) both bFGF and EGF show very effective neuroprotection for rat retinal neurones exposed to toxic levels of glutamate or its nonphysiological agonist kainate in vitro; (2) GM1 (10(-5M) used under the same conditions does not afford protection; (3) retinal glial cells also suffer morphological perturbations following glutamate or kainate treatment, but this effect is dependent on neuron-glial interactions, indicating the existence of intermediate neuron-derived messenger molecules; (4) these glial changes can be corrected by posttreatment with either bFGF or EGF in vitro; (5) using an in vivo animal model involving anterior chamber pressure-induced ischemia in adult rats, it is shown that either pretreatment by intraperitoneal injection of GM1, or posttreatment by intraocular injection of the same ganglioside, reduces significantly histological damage to inner nuclear regions; and (6) in cultured retinal Müller glial cells the existence of molecular and metabolic interactions between both types of trophic factors is demonstrated. Hence both these groups of trophic molecules show interesting features for retinal ischemic treatment.
Fast iterative image restoration with a spatially varying PSF (1997)

We describe how to efficiently apply a spatially-variant blurring operator using linear interpolation of measured point spread functions. Numerical experiments illustrate that substantially better resolution can be obtained at very little additional cost compared to piecewise constant interpolation.
Calcium regulation of keratinocyte differentiation (2012)

Calcium is the major regulator of keratinocyte differentiation in vivo and in vitro. A calcium gradient within the epidermis promotes the sequential differentiation of keratinocytes as they traverse the different layers of the epidermis to form the permeability barrier of the stratum corneum. Calcium promotes differentiation by both outside-in and inside-out signaling. A number of signaling pathways involved with differentiation are regulated by calcium, including the formation of desmosomes, adherens junctions and tight junctions, which maintain cell-cell adhesion and play an important intracellular signaling role through their activation of various kinases and phospholipases that produce second messengers that regulate intracellular free calcium and PKC activity, critical for the differentiation process. The calcium receptor plays a central role by initiating the intracellular signaling events that drive differentiation in response to extracellular calcium. This review will discuss these mechanisms.
Potential for specific cancer therapy with immune T lymphocytes. (1984)

In animal models, advanced disseminated tumors can be specifically eradicated by infusions of T lymphocytes immune to tumor. The most successful regimens require a relatively small tumor burden and immunosuppression of the host to augment the transfer of immunity. Since chemotherapeutic agents administered to tumor-bearing hosts can achieve both requirements, models that combine chemotherapy and immune cells have been developed and have been shown to be effective for the therapy of advanced syngeneic malignancies. Surprisingly, tumor eradication in these and similar models does not require the transfer of cytotoxic T lymphocytes. Rather, noncytotoxic T cells of the helper/inducer subset alone are sufficient, presumably mediating tumor elimination by a delayed-type hypersensitivity mechanism involving induction of host inflammatory cells. Since donor T cells must persist in the host to provide maximum benefit in therapy, the outcome of therapy is subject to both positive and negative influences within the host for a prolonged period of time. Interleukin-2 (IL-2), a T cell replication-inducing lymphokine, promotes the in vitro growth of antigen-activated T lymphocytes. T lymphocytes immune to tumor can be grown long-term in vitro in response to IL-2 and are able to mediate a specific, cell dose-dependent effect in therapy when inoculated into tumor-bearing hosts. In vitro such long-term cultured T lymphocytes are exquisitely dependent upon exogenous IL-2 for the induction of proliferation and survival. In vivo the administration of IL-2 induces the growth of similar long-term cultured T lymphocytes and, as a consequence, increases their therapeutic efficacy. Therefore, IL-2 can be utilized both in vitro and in vivo to augment specific T cell responses for the therapy of established tumors.
Experience with inhibitor injection to combat IGSCC in PWR steam generators (1995)

Since the rate of IGA/SCC of Alloy 600 in caustic solutions can be reduced in the presence of titanium compounds, field tests of such compounds were conducted at the Prairie Island 1 and Point Beach 2 PWRs. Behavior of titanium dioxide, titanium dioxide-silica sol-gel and an organic titanium chelate was assessed during these tests. Limited laboratory testing also was performed. No detrimental effects of titanium dioxide injection on plant chemistry were observed. However, only minimal titanium concentrations ({approx} 5 ppb as a particulate species) could be maintained in the steam generators.
B fibers are the best predictors of cardiac activity during Vagus nerve stimulation (2018)

B fiber activation can serve as a standardized, objective measure of stimulus dose across all subjects. Tracking fiber activation provides a more systematic way to study the effects of VNS and in the future, may lead to a more consistent method of therapy delivery.
Multimodality Imaging of Coiled‐Coil Mediated Self‐Assembly in a “Drug‐Free” Therapeutic System (2015)

Two complementary coiled-coil peptides CCE/CCK are used to develop a "drug free" therapeutic system, which can specifically kill cancer cells without a drug. CCE is attached to the Fab' fragment of anti-CD20 1F5 antibody (Fab'-CCE), and CCK is conjugated in multiple grafts to poly[N-(2-hydroxypropyl)methacrylamide] (P-(CCK)x ). Two conjugates are consecutively administered: First, Fab'-CCE coats peptide CCE at CD20 antigen of lymphoma cell surface; second, CCE/CCK biorecognition between Fab'-CCE and P-(CCK)x leads to coiled-coil formation, CD20 crosslinking, membrane reorganization, and ultimately cell apoptosis. To prove that two conjugates can assemble at cell surface, multiple fluorescence imaging studies are performed, including 2-channel FMT, 3D confocal microscopy, and 4-color FACS. Confocal microscopy shows colocalization of two fluorescently labeled conjugates on non-Hodgkin's lymphoma (NHL) Raji cell surface, indicating "two-step" targeting specificity. The fluorescent images also reveal that these two conjugates can disrupt normal membrane lipid distribution and form lipid raft clusters, leading to cancer cell apoptosis. This "two-step" biorecognition capacity is further demonstrated in a NHL xenograft model, using fluorescent images at whole-body, tissue and cell levels. It is also found that delaying injection of P-(CCK)x can significantly enhance targeting efficacy. This high-specificity therapeutics provide a safe option to treat NHL and other B cell malignancies.
Design of improved integrated thin-film planar dc SQUID gradiometers (1985)

Key issues in the design of improved first and second derivative, thin-film, planar dc SQUID gradiometers are discussed. The introduction of a planar coupling scheme to optimally couple the planar dc SQUID to the gradiometer pickup loops leads to significantly increased sensitivity as well as elimination of the sensitivity differences between series and parallel gradiometer loop configurations. Two-hole and figure-8 SQUID designs are presented which are consistent with intrinsic gradiometer balance ≲10−4 against uniform field changes. Straightforward calculations together with data from existing low-noise SQUIDs suggest improvements in gradient sensitivity on the order of 102 over existing planar gradiometers.
Kaposi's Sarcoma-Associated Herpesvirus Induces Nrf2 Activation in Latently Infected Endothelial Cells through SQSTM1 Phosphorylation and Interaction with Polyubiquitinated Keap1 (2014)

The transcription factor Nrf2 is activated by stress signals, including viral infection, and responds by activating the transcription of cytoprotective genes. Recently, Nrf2 has been implicated in oncogenesis and was shown to be activated during de novo KSHV infection of endothelial cells through ROS-dependent pathways. The present study was undertaken to determine the mechanism of Nrf2 activation during prolonged latent infection of endothelial cells, using an endothelial cell line latently infected with KSHV. We show that Nrf2 activation was elevated in KSHV latently infected endothelial cells independently of oxidative stress but dependent on the autophagic protein sequestosome-1 (SQSTM1), which was involved in the degradation of the Nrf2 inhibitor Keap1. Furthermore, our results indicated that the KSHV latent protein vFLIP participates in Nrf2 activation. This study suggests that KSHV hijacks the host's autophagic protein SQSTM1 to induce Nrf2 activation, thereby manipulating the infected host gene regulation to promote KS pathogenesis.