gebhart/patrepeval huggingface dataset:
Pertussis Toxin-Catalyzed ADP-Ribosylation: Effects on the Coupling of Inhibitory Receptors to the Adenylate Cyclase System (1984)

The adenylate cyclase system consists of stimulatory and inhibitory hormone and drug receptors coupled through different GTP-binding proteins to a catalytic unit, responsible for the synthesis of cAMP from ATP. Pertussis toxin blocks the effect of inhibitory agonists on the catalytic unit by enzymatically inactivating the inhibitory GTP-binding protein (Gi). Study of the inhibitory arm of the cyclase system has been facilitated by the dissection of the overall process of hormonal inhibition of cAMP formation into a series of reactions characteristic of the individual protein components of this complex system; pertussis toxin has proven to be a useful tool with which to study these individual reactions. Exposure of cells or membranes to pertussis toxin in the presence of NAD results in ADP-ribosylation of a 41,000 Da subunit of Gi. ADP-ribosylation of Gi has a number of effects on the overall and partial reactions of the cyclase system, including a loss of a) hormonal inhibition of cAMP formation, b) hormonal stimulation of GTPase and c) agonist-induced release of membrane-bound guanyl nucleotides. In addition, in toxin-treated membranes, the affinity of inhibitory receptors for agonist but not antagonist is decreased with no significant change in receptor number.
Low-loss polarization-maintaining fusion splicing of single-mode fibers and hollow-core photonic crystal fibers, relevant for monolithic fiber laser pulse compression (2008)

We report on highly reproducible low-loss fusion splicing of polarization-maintaining single-mode fibers (PM-SMFs) and hollow-core photonic crystal fibers (HC-PCFs). The PM-SMF-to-HC-PCF splices are characterized by the loss of 0.62 +/- 0.24 dB, and polarization extinction ratio of 19 +/- 0.68 dB. The reciprocal HC-PCF-to-PM-SMF splice loss is found to be 2.19 +/- 0.33 dB, which is caused by the mode evolution in HC-PCF. The return loss in both cases was measured to be -14 dB. We show that a splice defect is caused by the HC-PCF cleave defect, and the lossy splice can be predicted at an early stage of the splicing process. We also demonstrate that the higher splice loss compromises the PM properties of the splice. Our splicing technique was successfully applied to the realization of a low-loss, environmentally stable monolithic PM fiber laser pulse compressor, enabling direct end-of-the-fiber femtosecond pulse delivery.
On the problem of regioselectivity in the 1,3-dipolar cycloaddition reaction of munchnones and sydnones with acetylenic dipolarophiles (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOn the problem of regioselectivity in the 1,3-dipolar cycloaddition reaction of munchnones and sydnones with acetylenic dipolarophilesAlbert Padwa, Edward M. Burgess, Henry L. Gingrich, and David M. RoushCite this: J. Org. Chem. 1982, 47, 5, 786–791Publication Date (Print):February 1, 1982Publication History Published online1 May 2002Published inissue 1 February 1982https://pubs.acs.org/doi/10.1021/jo00344a008https://doi.org/10.1021/jo00344a008research-articleACS PublicationsRequest reuse permissionsArticle Views753Altmetric-Citations72LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
The "molecular movements" of sensitive moving-magnet galvanometers (1926)

A discussion is given of the random movements of the system of a sensitive moving magnet galvanometer, on the assumption that these have a mean energy equal to 1/3 of that of a gas molecule at the same temperature. It is shown that these movements are of importance at sensitivities now practically attainable, and certain deductions are made in connexion with the design of galvanometer systems.
Role of the target in directing the outgrowth of retinal axons: Transplants reveal surface‐related and surface‐Independent cues (1987)

In the present investigation we have examined whether retinal axons can be directed to the superior colliculus via an alternate route when they do not have access to their normal substrates along the optic tract. To address this issue we transplanted embryonic mouse retinae into the midbrain parenchyma and to various positions around the outer surface of the midbrain of newborn rats and then examined the development of projections from the transplanted tissue. The projections from cortical grafts placed in the midbrain were studied to determine whether axons from different classes of neurons respond to the same cues for outgrowth. When retinae were placed within the midbrain close to the cerebral aqueduct, axons projected dorsally to the superficial layers of the superior colliculus. Directed outgrowth was seen from the earliest time a projection could be detected and was independent of whether the superior colliculus still received host optic afferents. In contrast, the major projection from similarly placed cortical transplants was directed toward the ventral part of the midbrain. Deafferentation of midbrain corticorecipient areas did not affect the projection patterns from either type of graft. Projections from retinae placed more ventrally in the midbrain tegmentum could not be detected. However, retinae placed on the surface of the midbrain, even as far ventral as the cerebral peduncle at the level of the inferior colliculus, always had a projection to the superior colliculus that ran along the brainstem surface. These observations suggest that the superior colliculus exerts a positive influence on the growth of optic axons to the midbrain. However, while target cues appear to be able to support retinal axon growth through the midbrain parenchyma, their range appears to be limited, and at distances beyond the extent of their influence, optic fiber outgrowth occurs only over the surface of the brain. It is suggested, therefore, that there are both local surface-related and target-derived surface-independent cues that guide optic axons to the tectum in developing mammals.
A flow cytometric study of antibiotic-induced damage and evaluation as a rapid antibiotic susceptibility test for methicillin-resistant Staphylococcus aureus (1997)

Flow cytometry using the anionic membrane potential-sensitive fluorescent probe, bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)), enabled assessment of antibiotic-induced membrane perturbation in five clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and two antibiotic-sensitive reference strains, NCTC 6571 and 8325-4, after establishment of steady-state growth in liquid cultures inoculated from single colonies. Flow cytometric indications of the enhanced DiBAC4(3) uptake after treatment with vancomycin at 0.1, 1, 4 and 10 x MIC showed excellent comparison with viability losses quantified as cfu on solid agar in MRSA isolate QC. The antibiotic susceptibility patterns to benzylpenicillin, methicillin and vancomycin for all isolates used in this study could be determined in 2-4 h from an overnight plate culture. This technique thus provides a rapid and reproducible antibiotic sensitivity test which may be applicable in routine clinical practice.
INVESTIGATION ON THE SCATTERING CHARACTERISTICS OF GAUSSIAN BEAM FROM TWO DIMENSIONAL DIELECTRIC ROUGH SURFACES BASED ON THE KIRCHHOFF APPROXIMATION (2008)

The scattering characteristic of paraxial gaussian beam from two dimensional dielectric rough surfaces is studied in this paper. The modification of the Kirchhoff approximation theory for rough surface scattering by an incident gaussian beam instead of a plane wave are developed based on conventional Kirchhoff scattering theory and plane wave spectrum expansion method. The coherent and incoherent scattered intensity and cross section of two dimensional dielectric rough surfaces is derived in detail. As a application, under incidence wave length λ =1 .06 µm, we calculate the coherent and incoherent scattered intensity and cross section of Gaussian beam scattering from plating aluminium dielectric rough surfaces change with the scattering zenith angles in different rough surface correlation length, rough surface height root mean square and other conditions. In the same scattering conditions, we compare the coherent and incoherent scattered section between the gaussian beam and plane wave to prove that our methods and programming cods is correct. The numerical results are shown
Occupancy-elevation grid: an alternative approach for robotic mapping and navigation (2015)

SUMMARY This paper proposes an alternative environment mapping method for accurate robotic navigation based on 3D information. Typical techniques for 3D mapping using occupancy grid require intensive computational workloads in order to both build and store the map. This work introduces an Occupancy-Elevation Grid (OEG) mapping technique, which is a discrete mapping approach where each cell represents the occupancy probability, the height of the terrain and its variance. This representation allows a mobile robot to know with an accurate degree of certainty whether a place in the environment is occupied by an obstacle and the height of such obstacle. Thus, based on its hardware characteristics, it can make calculations to decide if it is possible to traverse that specific place. In general, the map representation introduced can be used in conjunction with any kind of distance sensor. In this work, we use laser range data and stereo system data with a probabilistic treatment. The resulting maps allow the execution of tasks as decision making for autonomous navigation, exploration, localization and path planning, considering the existence and the height of the obstacles. Experiments carried out with real data demonstrate that the proposed approach yields useful maps for autonomous navigation.
Analysis of Direct P(Y)-Code Acquisition (1997)

GPS signal acquisition consists of a two-dimensional search in a time/frequency space to synchronize a receiver-generated reference signal with the received GPS signal in code phase and frequency. User uncertainties in code phase and frequency offsets determine the size of the region to be searched, and hence the code acquisition time. Encrypted P(Y)-code, transmitted on L1 and L2 frequencies, provides more precise (16 m spherical error probable [SEP]) user positioning and inherently more jamming immunity than does C/A-code. The purpose of this study is to explore the use of existing signal detection concepts for quantifying the time to achieve direct acquisition of the P(Y)-code in a jamming environment. Of particular interest is the mitigating effect of parallel processing in reducing acquisition time in the presence of initial (receiver) position, time, velocity, and frequency errors and/or uncertainties.
cGMP becomes a drug target (2012)

Cyclic guanosine 3',5'-monophosphate (cGMP) serves as a second messenger molecule, which regulates pleiotropic cellular functions in health and disease. cGMP is generated by particulate or soluble guanylyl cyclases upon stimulation with natriuretic peptides or nitric oxide, respectively. Furthermore, the cGMP concentration is modulated by cGMP-degrading phosphodiesterases. Several targets of cGMP are utilized to effect its various cellular functions. These effector molecules comprise cGMP-dependent protein kinases, ion channels, and phosphodiesterases. During the last decade, it emerged that cGMP is a novel drug target for the treatment of pulmonary and cardiovascular disorders. In this respect, several drugs were developed, which are now in clinical phase studies for, e.g., pulmonary hypertension or cardiovascular diseases. These new drugs act NO-independently with/without heme on soluble guanylyl cyclases or induce subtypes of particular guanylyl cyclases and thereby lead to new therapeutic concepts and horizons. In this regard, the fifth cGMP meeting held in June 2011 in Halle, Germany, comprised the new therapeutic challenges with the novel functional and structural concepts of cGMP generating and effector molecules. This report summarizes the new data on molecular mechanisms, (patho)physiological relevance, and therapeutic potentials of the cGMP signaling system that were presented at this meeting.
Resource utilisation and direct costs in patients with recently diagnosed fibromyalgia who are offered one of three different interventions in a randomised pragmatic trial (2015)

The purpose of this study is to understand the course of costs over a 2-year period in a cohort of recently diagnosed fibromyalgia (FM) patients receiving different treatment strategies. Following the diagnosis, patients were randomly assigned to a multidisciplinary programme (MD), aerobic exercise (AE) or usual care (UC) without being aware of alternative interventions. Time between diagnosis and start of treatment varied between patients. Resource utilisation, health care costs and costs for patients and families were collected through cost diaries. Mixed linear model analyses (MLM) examined the course of costs over time. Linear regression was used to explore predictors of health care costs in the post-intervention period. Two hundred three participants, 90 % women, mean (SD) age 41.7 (9.8) years, were included in the cohort. Intervention costs per patient varied from €864 to 1392 for MD and were €121 for AE. Health care costs (excluding intervention costs) decreased after diagnosis, but before the intervention in each group, and increased again afterwards to the level close to the diagnostic phase. In contrast, patient and family costs slightly increased over time in all groups without initial decrease immediately after diagnosis. Annualised health care costs post-intervention varied between €1872 and 2310 per patient and were predicted by worse functioning and high health care costs at diagnosis. In patients with FM, health care costs decreased following the diagnosis by a rheumatologist. Offering patients a specific intervention after diagnosis incurred substantial costs while having only marginal effects on costs.
Symposium review: Features of Staphylococcus aureus mastitis pathogenesis that guide vaccine development strategies (2018)

Bovine mastitis affects animal health and welfare and milk production and quality, and it challenges the economic success of dairy farms. Staphylococcus aureus is one of the most commonly found pathogens in clinical mastitis but it also causes subclinical, persistent, and difficult-to-treat intramammary infections. Because of the failure of conventional antibiotic treatments and increasing pressure and concern from experts and consumers over the use of antibiotics in the dairy industry, many attempts have been made over the years to develop a vaccine for the prevention and control of Staph. aureus intramammary infections. Still, no commercially available vaccine formulation demonstrates sufficient protection and cost-effective potential. Multiple factors account for the lack of protection, including inadequate vaccine targets, high diversity among mastitis-provoking strains, cow-to-cow variation in immune response, and a failure to elicit an immune response that is appropriate for protection against a highly complex pathogen. The purpose of this review is to summarize key concepts related to the pathogenesis of Staph. aureus, and its interaction with the host, as well as to describe recent vaccine development strategies for prevention and control of Staph. aureus mastitis.
An Autonomous Multi-UAV System for Search and Rescue (2015)

This paper proposes and evaluates a modular architecture of an autonomous unmanned aerial vehicle (UAV) system for search and rescue missions. Multiple multicopters are coordinated using a distributed control system. The system is implemented in the Robot Operating System (ROS) and is capable of providing a real-time video stream from a UAV to one or more base stations using a wireless communications infrastructure. The system supports a heterogeneous set of UAVs and camera sensors. If necessary, an operator can interfere and reduce the autonomy. The system has been tested in an outdoor mission serving as a proof of concept. Some insights from these tests are described in the paper.
Circular genomes related to anelloviruses identified in human and animal samples by using a combined rolling-circle amplification/sequence-independent single primer amplification approach (2007)

A combined rolling-circle amplification (RCA) and sequence-independent single primer amplification (SISPA) approach was applied to four samples of human plasma and one sample of saliva from a cat. This approach permitted the characterization of nine anelloviruses. Most of them were identified as highly divergent strains that were classified into species of the genus Anellovirus. The smallest anellovirus described so far in humans was characterized (2PoSMA, 2002 nt; 'small anellovirus' species). Two highly divergent sequences belonging to the species Torque Teno Mini Virus (LIL-y1, 2887 nt; LIL-y2, 2871 nt), which clustered into a new phylogenetic branch, were also identified in human plasma samples. Finally, two genomes that are separated by a genetic divergence of 46 % were characterized in the cat's saliva, one of these creating a distinct phylogenetic branch (PRA1, 2019 nt). These results highlight the potential of RCA-SISPA for detecting circular (or circularized) genomes.
A 1-tap 40-Gbps look-ahead decision feedback equalizer in 0.18 /spl mu/m SiGe BiCMOS technology (2005)

This paper describes a fully-differential 1-Tap decision feedback equalizer (DFE) in 0.18/spl mu/m SiGe BiCMOS technology. The circuit is capable of equalizing NRZ data up to 40 Gbps. A look-ahead architecture is used with modifications to reduce complexity in the high speed clock distribution. An analog differential voltage controls the tap weights. The design is fabricated in 0.18 /spl mu/m SiGe BiCMOS technology with a 160-GHz ft. It occupies an area of 1.5 mm /spl times/ 1mm and operates from a 3.3 V supply with 230 mA current. It is the first feedback equalizer at 40 Gbps in silicon.
Anthropomorphic 1H MRS head phantom (1998)

An anthropomorphic 1H MRS head phantom has been developed which mimics the in vivo structure, metabolite concentrations, and relaxation times (for both water and metabolites) of human brain tissue. Different brain regions and two tumor types, fluid-containing ventricles, and air-filled sinus, and subcutaneous fat are all simulated. The main tissue-mimicking materials are gelatin/agar mixtures with metabolites and several other ingredients added. Their composition and method of production are thoroughly described. T1's and T2's of water in the phantom are very close to in vivo values, and metabolite T1's and T2's are considerably more realistic than those in aqueous solutions. Spectra and relaxation times for the pig brain were also acquired and compare well with those of the phantom. The realistic properties of this phantom should be useful for testing spectral quantitation and localization.
Estimation of Frequency for AM/FM Models Using the Phase Vocoder Framework (2008)

This paper proposes an extension of the applicability of phase-vocoder-based frequency estimators for generalized sinusoidal models, which include phase and amplitude modulations. A first approach, called phase corrected vocoder (PCV), takes into account the modification of the Fourier phases resulting from these modulations. Another approach is based on an adaptation of the principles of the time-frequency reassignment and is referred to as the reassigned vocoder (RV). The robustness of the estimation against noise is studied, both theoretically and experimentally, and the performance is assessed in comparison with two state-of-the-art algorithms: an unmodified version of the reassignment method and a quadratically interpolated fast Fourier transform method (QIFFT).
Shock induced polymorphic transition in quartz, carbon, and boron nitride (1990)

A theory describing the polymorphism induced by shock waves in silicates, oxides, sulfides, and many inorganic solids is presented. Shock wave experiments conducted on these and other materials indicate that many transformations to high-pressure phases are triggered via the production of shear bands and, in some cases, formation of high-density amorphous phases. Shock states in the mixed phase regimes, of quartz, carbon, and boron nitride, are quantitatively described in terms of the properties of both their low- and high-pressure phases. Good agreement between the calculated results and measured Hugoniot data in the mixed phase regime is obtained. By fitting the pressures of the onset of the phase transition from graphite to diamond, and associating its triggering with crossing the extension of the metastable melting line of graphite, we obtain a similar shaped curve to the metastable melting line obtained by Bundy [J. Geophys. Res. 85, 6930 (1980)]. Similarly, the transition from quartz to stishovite is associated with the metastable melting line of coesite. The present theory, when fit to the onset of the mixed phase regime of graphitelike boron nitride transforming to cubic boron nitride Hugoniot, predicts the standard entropy for cubic BN to be 0.4–0.5 J/g K.
Synthesis of methyl ketones by metabolically engineered <i>Escherichia coli</i> (2012)

Methyl ketones are a group of highly reduced platform chemicals with widespread applications in the fragrance, flavor and pharmacological industries. Current methods for the industrial production of methyl ketones include oxidation of hydrocarbons, but recent advances in the characterization of methyl ketone synthases from wild tomato have sparked interest towards the development of microbial platforms for the industrial production of methyl ketones. A functional methyl ketone biosynthetic pathway was constructed in Escherichia coli by over-expressing two genes from Solanum habrochaites: shmks2, encoding a 3-ketoacyl-ACP thioesterase, and shmks1, encoding a beta-decarboxylase. These enzymes enabled methyl ketone synthesis from 3-ketoacyl-ACP, an intermediate in the fatty acid biosynthetic cycle. The production of 2-nonanone, 2-undecanone, and 2-tridecanone by MG1655 pTH-shmks2-shmks1 was initially detected by nuclear magnetic resonance and gas chromatography-mass spectrometry analyses at levels close to 6 mg/L. The deletion of major fermentative pathways leading to ethanol (adhE), lactate (ldhA), and acetate (pta, poxB) production allowed for the carbon flux to be redirected towards methyl ketone production, doubling total methyl ketone concentration. Variations in methyl ketone production observed under different working volumes in flask experiments led to a more detailed analysis of the effects of oxygen availability on methyl ketone concentration in order to determine optimal levels of oxygen. The methyl ketone concentration achieved with MG1655 ∆adhE ∆ldhA ∆poxB ∆pta pTrcHis2A-shmks2-shmks1, the best performer strain in this study, was approximately 500 mg/L, the highest reported for an engineered microorganism. Through the establishment of optimal operating conditions and by executing rational metabolic engineering strategies, we were able to increase methyl ketone concentrations by almost 75-fold from the initial confirmatory levels.