gebhart/patrepeval huggingface dataset:
Characteristics of Biofilm Formation by<i>Streptococcus mutans</i>in the Presence of Saliva (2008)

Interactions between salivary agglutinin and the adhesin P1 of Streptococcus mutans contribute to bacterial aggregation and mediate sucrose-independent adherence to tooth surfaces. We have examined biofilm formation by S. mutans UA159, and derivative strains carrying mutations affecting the localization or expression of P1, in the presence of fluid-phase or adsorbed saliva or salivary agglutinin preparations. Whole saliva- and salivary agglutinin-induced aggregation of S. mutans was adversely affected by the loss of P1 and sortase (SrtA) but not by the loss of trigger factor (RopA). Fluid-phase salivary agglutinin and, to a lesser extent, immobilized agglutinin inhibited biofilm development by S. mutans in the absence of sucrose, and whole saliva was more effective at decreasing biofilm formation than salivary agglutinin. Inhibition of biofilm development by salivary agglutinin was differently influenced by particular mutations, with the P1-deficient strain displaying a greater inhibition of biofilm development than the SrtA- or RopA-deficient strains. As expected, biofilm-forming capacities of all strains in the presence of salivary preparations were markedly enhanced in the presence of sucrose, although biofilm formation by the mutants was less efficient than that by the parental strain. Aeration strongly inhibited biofilm development, and the presence of salivary components did not restore biofilm formation in aerated conditions. The results disclose a potent ability of salivary constituents to moderate biofilm formation by S. mutans through P1-dependent and P1-independent pathways.
Detection and Analysis of Mitochondrial DNA Deletions by Whole Genome PCR (1996)

Southern blot analysis has been the best method available for the screening and detection of mitochondrial DNA (mtDNA) rearrangements. Recent developments in polymerase chain reaction (PCR) technology allowed the amplification of the whole mitochondrial genome (16.6 kb), making PCR a potentially useful technique for the detection of mtDNA deletions. We tested the usefulness of whole mitochondrial genome PCR by studying skeletal muscle DNA from seven patients with single and multiple deletions and controls from ages 3 to 91 years old. Specific patterns for single and multiple deletions were obtained with whole genome PCR, which were confirmed by the Southern analysis with probes hybridizing to mtDNA sequences. Amplifications from young controls (3 to 23 years old) yielded only one band (16.5 kh) while amplification from older controls revealed one or more additional smaller bands. The amplification from the 91-year-old control showed a pattern similar to amplifications from patients with multiple mtDNA deletions. Although single and multiple mtDNA deletions could be readily detected from patient samples, the high sensitivity of this method can lead to false positive results due to the presence of age-related deletions in old control samples. Despite its limitations, whole mitochondrial genome PCR can be useful for the detection of single deletions in muscle samples obtained from young individuals since the levels of age-related deletions are too low to be amplified.
Electroosmotic Flow Reversal Outside Glass Nanopores (2014)

We report observations of a striking reversal in the direction of electroosmotic flow (EOF) outside a conical glass nanopore as a function of salt concentration. At high ionic strengths (>100 mM), we observe EOF in the expected direction as predicted by classical electrokinetic theory, while at low salt concentrations (<1 mM) the direction of the flow is reversed. The critical crossover salt concentration depends on the pore diameter. Finite-element simulations indicate a competition between the EOF generated from the inner and outer walls of the pore, which drives flows in opposite directions. We have developed a simple analytical model which reveals that, as the salt concentration is reduced, the flow rates inside the pore are geometrically constrained, whereas there is no such limit for flows outside the pore. This model captures all of the essential physics of the system and explains the observed data, highlighting the key role the external environment plays in determining the overall electroosmotic behavior.
New Species of Ericentrodea from Bolivia and Colombia (Asteraceae, Coreopsidinae, Heliantheae) (1993)

Ericentrodea davidsmithii from Bolivia and E. ramirezii from Colombia are described. A key is provided for the six known species of the Andean genus Ericentrodea.
ChemInform Abstract: Azole Endothelin Antagonists. Part 1. A Receptor Model Explains an Unusual Structure‐Activity Profile. (1996)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
<title>Multimedia medical data archive and retrieval server on the Internet</title> (1997)

The Multimedia Medical Data Archive and Retrieval Server has been installed at the imaging science and information systems (ISIS) center in Georgetown University Medical Center to provide medical data archive and retrieval support for medical researchers. The medical data includes text, images, sound, and video. All medical data is keyword indexed using a database management system and placed temporarily in a staging area and then transferred to a StorageTek one terabyte tape library system with a robotic arm for permanent archive. There are two methods of interaction with the system. The first method is to use a web browser with HTML functions to perform insert, query, update, and retrieve operations. These generate dynamic SQL calls to the database and produce StorageTek API calls to the tape library. The HTML functions consist of a database, StorageTek interface, HTTP server, common gateway interface, and Java programs. The second method is to issue a DICOM store command, which is translated by the system's DICOM server to SQL calls and then produce StorageTek API calls to the tape library. The system performs as both an Internet and a DICOM server using standard protocols such as HTTP, HTML, Java, and DICOM. Users with proper authentication can log on to the server from anywhere on the Internet using a standard web browser resulting in a user-friendly, open environment, and platform independent solution for archiving multimedia medical data. It represents a complex integration of different components including a robotic tape storage system, database, user-interface, WWW protocols, and TCP/IP networking. The user will only deal with the WWW and DICOM server components of the system, the database and robotic tape library system are transparent and the user will not know that the medical data is stored on magnetic tapes. The server provides the researchers a cost-effective tool for archiving and retrieving medical data across a TCP/IP network environment. It will serve as a medium to exchange information between researchers at Georgetown University and those from other institutions.
Charging of large structures in space with application to the solar sail spacecraft (1985)

Covers advancements in spacecraft and tactical and strategic missile systems, including subsystem design and application, mission design and analysis, materials and structures, developments in space sciences, space processing and manufacturing, space operations, and applications of space technologies to other fields.
The Restriction of Bacteriophage   in Escherichia coli Strain w (1967)

Summary Escherichia coli strain w adsorbs phage λ very efficiently but the phage does not form plaques on this strain. In a very small fraction (10−4) of the infected cells the phage grows and produces small bursts of progeny phage also unable to form plaques on strain w. E. coli strain w is lysogenic for a temperate phage, wϕ, related to phage p2. Non-restricting hosts for phage λ became restricting hosts when made lysogenic for wϕ. When 32P-labelled λ adsorbed to restricting wϕ lysogenic hosts, > 20% of the 32P become acid-soluble shortly after infection. No wϕ specific modification was carried by the small number of λ Phages which escaped this restriction process. It is concluded that wϕ controls a host-restriction mechanism but not a host-modification process, and in parallel with other examples of host-controlled restriction and modification can be represented as r + m − or r + m°. λw mutants have been isolated which escape this restriction and which form plaques on strain w and w wϕ lysogenic strains with an efficiency of 1·. With these mutants a w-specific host modification controlled by the genome of strain w was demonstrated. Mixed infection experiments with restricted λ and unrestricted λw showed that that restricted phage did not block the growth of the unrestricted mutant nor did the mutant permit the restricted phage to grow. In addition it was shown that λ obtained from bacteria mixedly infected with λ and λw was still unable to grow in restricting hosts and λw similarly obtained from mixedly infected bacteria still retained its ability to grow on restricting hosts. It is concluded that there is a nucleotide sequence in the DNA of phage λ which, when λ infects a restricting host, is specifically recognized by the restriction mechanism controlled by the wϕ. The mutation to λw involves an alteration to this sequence such that it is no longer recognized by the restriction mechanism of the wϕ. Mutants of wϕwere isolated not restrictive for phage λ.
JavaBeans Developer's Reference (with CD-ROM) (1997)

From the Publisher:
JavaBeans Developer s Reference is the one-stop source for experienced professional Java developers seeking concise information on building, packaging, using, and updating JavaBean components. You ll learn the intricacies of extending JavaBeans into environments where most developers fear to tread. Bring your JavaBean components to the global marketplace armed with knowledge on component internalization, custom editing capabilities, integrity, and flexibility. Get the upper hand on the competition or cooperate with other developers to design dynamic JavaBeans that work in tandem. Regardless of the path you follow as a Beans developer, all the information you need -- from intuitive tutorials to extensive coverage of the JavaBeans and related APIs -- lies within the pages of the JavaBeans Developer s Reference. } Master the art of JavaBean development and packaging with step-by-step intuitive tutorials } Understand the impact and implications of JavaBeans on the software component architecture industry } Learn how to connect multiple JavaBeans for powerhouse components } Empower your JavaBeans with global accessibility through Internalization } Learn to handle universal JavaBean development issues by exploiting the Beans Development Kit } Personalize your JavaBeans by building your own custom component editors } Arm your JavaBeans to handle the Java 1.1 event model } Learn to ensure JavaBean integrity with Persistence } Take advantage of the extensive JavaBeans reference material as you develop CD-ROM includes: JDK 1.1: A must-have for JavaBean developers BDK: The Bean Development Kit that includes the BeanBox application as well as 12 example Beans Source code from the book: use the files from the book or customize them to suit your own needs Daniel Brookshier is a Software Engineering Consultant with over 15 years of experience and is a well-respected member of the Java community. He has worked on a wide range of industry applications, including telecommunications, oil & gas, aerospace, insurance, sales support, and software design tools.
Provides detailed coverage of how to create and implement JavaBeans
Gain insight into the Java APIs created to support JavaBeans
The companion CD-ROM includes examples from the book and an electronic version of the API reference section
A Conserved Mechanism for Steroid Receptor Translocation to the Plasma Membrane (2007)

Multiple steroid receptors (SR) have been proposed to localize to the plasma membrane. Some structural elements for membrane translocation of the estrogen receptor alpha (ER alpha) have been described, but the mechanisms relevant to other steroid receptors are entirely unknown. Here, we identify a highly conserved 9 amino acid motif in the ligand binding domains (E domains) of human/mouse ER alpha and ER beta, progesterone receptors A and B, and the androgen receptor. Mutation of the phenylalanine or tyrosine at position-2, cysteine at position 0, and hydrophobic isoleucine/leucine or leucine/leucine combinations at positions +5/6, relative to cysteine, significantly reduced membrane localization, MAP and PI 3-kinase activation, thymidine incorporation into DNA, and cell viability, stimulated by specific SR ligands. The localization sequence mediated palmitoylation of each SR, which facilitated caveolin-1 association, subsequent membrane localization, and steroid signaling. Palmitoylation within the E domain is therefore a crucial modification for membrane translocation and function of classical sex steroid receptors.
ASAR15, A cis-Acting Locus that Controls Chromosome-Wide Replication Timing and Stability of Human Chromosome 15 (2015)

DNA replication initiates at multiple sites along each mammalian chromosome at different times during each S phase, following a temporal replication program. We have used a Cre/loxP-based strategy to identify cis-acting elements that control this replication-timing program on individual human chromosomes. In this report, we show that rearrangements at a complex locus at chromosome 15q24.3 result in delayed replication and structural instability of human chromosome 15. Characterization of this locus identified long, RNA transcripts that are retained in the nucleus and form a "cloud" on one homolog of chromosome 15. We also found that this locus displays asynchronous replication that is coordinated with other random monoallelic genes on chromosome 15. We have named this locus ASynchronous replication and Autosomal RNA on chromosome 15, or ASAR15. Previously, we found that disruption of the ASAR6 lincRNA gene results in delayed replication, delayed mitotic condensation and structural instability of human chromosome 6. Previous studies in the mouse found that deletion of the Xist gene, from the X chromosome in adult somatic cells, results in a delayed replication and instability phenotype that is indistinguishable from the phenotype caused by disruption of either ASAR6 or ASAR15. In addition, delayed replication and chromosome instability were detected following structural rearrangement of many different human or mouse chromosomes. These observations suggest that all mammalian chromosomes contain similar cis-acting loci. Thus, under this scenario, all mammalian chromosomes contain four distinct types of essential cis-acting elements: origins, telomeres, centromeres and "inactivation/stability centers", all functioning to promote proper replication, segregation and structural stability of each chromosome.
Clima futuro andaluz a través de un estudio sobre escenarios locales de cambio climático (2014)

espanolPredicciones de los efectos del cambio climatico en Andalucia realizadas por Rediam a partir de modelos de simulacion. EnglishPredictions of the effects of the climate change in Andalucia realized by Rediam with simulation models.
Effective thermal conductivity of particulate composites with interfacial thermal resistance (1997)

A methodology is introduced for predicting the effective thermal conductivity of arbitrary particulate composites with interfacial thermal resistance in terms of an effective medium approach combined with the essential concept of Kapitza thermal contact resistance. Results of the present model are compared to existing models and available experimental results. The proposed approach rediscovers the existing theoretical results for simple limiting cases. The comparisons between the predicted and experimental results of particulate diamond reinforced ZnS matrix and cordierite matrix composites and the particulate SiC reinforced Al matrix composite show good agreement. Numerical calculations of these different sets of composites show very interesting predictions concerning the effects of the particle shape and size and the interfacial thermal resistance.
Dynamic stereo vision (1989)

Sensing 3-D shape and motion is an important problem in autonomous navigation and manipulation. Stereo vision is an attractive approach to this problem in several domains. In this thesis, I address fundamental components of this problem by using stereo vision to estimate the 3-D structure of of objects visible to a robot, as well as to estimate the motion of the robot as its travels through an unknown environment.
I begin by using cameras on-board a robot vehicle to estimate the motion of the vehicle by tracking 3-D feature-points or landmarks. I formulate this task as a statistical estimation problem, develop sequential methods for estimating the vehicle motion and updating the landmark model, and implement a system that successfully tracks landmarks through stereo image sequences. In laboratory experiments, this system has achieved an accuracy of 2$\\$% of distance over 5.5 meters and 55 stereo image pairs. These results establish the importance of statistical modelling in this problem and demonstrate the feasibility of visual motion estimation in unknown environments.
This work embodies a successful paradigm for feature-based and motion estimation, but the feature-based approach results in a very limited 3-D model of the environment. To extend this aspect of the system, I address the problem of estimating depth from stereo images. Depth maps specify scene for each pixel in the image. I propose a system architecture in which exploratory camera motion is used to acquire a narrow-baseline image pair by moving one camera of the stereo system. Depth estimates obtained from this image pair are used to matching of a wide-baseline image pair acquired with both cameras of the system. I formulate the bootstrap operation statistically by modelling maps as random fields and developing Bayesian matching algorithms in which information from the narrow-baseline image pair determines the prior density for matching the wide baseline image pair. This leads to efficient, area-based matching algorithms that are applied independently for each pixel or each scanline of the image. Experimental results with images of complex, outdoor scene models demonstrate the power of the approach.
Effects of Garlic Compounds Diallyl Sulfide and Diallyl Disulfide on Arylamine N-Acetyltransferase Activity in Strains of<i>Helicobacter pylori</i>from Peptic Ulcer Patients (1998)

Arylamine N-acctyltransferase (NAT) activities with p-aminobenzoic acid (PABA) and 2-aminofluorene (2-AF) were determined in the bacterium Helicobacter pylori collected from peptic ulcer patients. Two assay systems were performed, one with cellular cytosols, the other with intact cell suspensions. Cytosols or suspensions of H. pylori with or without specific concentrations of diallyl sulfide (DAS) or diallyl disulfide (DADS) co-treatment showed different percentages of 2-AF and PABA acetylation. The data indicated that there was decreased NAT activity associated with increased levels of DAS or DADS in H. pylori cytosols and suspensions. Viability studies on H. pylori demonstrated that DAS or DADS elicited dose-dependent bactericide affects on H. pylori cultures. The data also indicated that DAS and DADS decreased the apparent values of K(m) and Vmax of NAT enzyme from H. pylori in both systems examined. This report is the first demonstration that garlic components can affect H. pylori growth and NAT activity.
Determination of material removal rate of an electro-discharge machine using dimensional analysis (2004)

This paper describes the use of dimensional analysis in investigating the effects of the electrical and the physical parameters on the material removal rate of a diesinking electro-discharge machine. A brief explanation of the material removal process is presented and the factors influencing the material removal rate are identified and used in the dimensional analysis to produce a mathematical model for the material removal rate. The validity of the analysis is verified since it predicts results which are in good agreement with experimental findings.
The discrete residence time distribution of a distillation colum operated with microprocessor controlled periodic cycling (1978)

Abstract The fluid flow in a distillation column was studied by measurements of the discrete residence time distribution. The column, 100 mm in diameter was fitted with 5 sieve plates of 14.8% free area with a 6.1 mm hole diameter on a 635 mm plate spacing. The periodic control was obtained using a JOLT microprocessor system. An analysis of the discrete residence time distribution yielded the parameters in the (2S) model that describes the fluid flow. Modifications to the column internals are required to alter these parameters, if the maximum separation improvements are to be obtained.
Testing the Role of Charge and Structure on the Stability of Peptide–Porphyrin Complexes (2014)

This study aims to extend a structural and biophysical understanding of a coiled-coil based peptide model system that serves as a scaffold for the anionic porphyrin, TPPS4. This is part of an ongoing biomaterials effort to create photoelectronically active mesoscale fibrils for surface deposition and characterization of conductivity properties. The goals are two-fold: (1) to explore optimal basic side-chain moieties for tight binding to TPPS4 and (2) to test the binding of various metalated TPPS4 derivatives to our peptide model system. The latter goal is to control the electronic and redox properties of the fibrillar biomaterials. A soluble version of the peptide biomaterial was used in order to probe binding and to extract thermodynamically rigorous equilibrium binding constants. UV-visible spectroscopy and circular dichroism spectropolarimtery are used to measure the effects of binding on the Soret band of the porphyrin and the helical signal of the peptide, respectively. For the first study, it was found that lysine, ornithine, and arginine are equally robust at engaging TPPS4 with low micromolar binding affinity. In the case of the metalated porphyrins, submicromolar binding affinity was observed for Cu(II), Ni(II), and Pd(II). The ability of these metalated porphyrins to bind with high affinity is dependent largely on structural perturbations of the porphyrin molecule, rather than on induced electronic effects.
Large strain transduction utilizing phase transition in relaxor-ferroelectric Pb(In1/2Nb1/2)O3–Pb(Mg1/3Nb2/3)O3–PbTiO3 single crystals (2011)

In this work, we present experimental evidence that under relatively low level drive (&lt;0.1 MV/m) the large strain (∼0.5%) associated with ferroelectric rhombohedral FR-ferroelectric orthorhombic FO phase transition in domain engineered relaxor–ferroelectric single crystals under compressive stress and bias electric field can be captured. We have demonstrated this in mechanically confined ternary Pb(In1/2Nb1/2)O3–Pb(Mg1/3Nb2/3)O3–PbTiO3 single crystals poised at the rhombohedral side of the morphotropic phase boundary. Experimental strain-field results, methods of mechanical confinement and drive, and a low frequency, compact, high source level sound projector design are presented. Transducers that operate on a large strain principle as a design rule will benefit from this development.