gebhart/patrepeval huggingface dataset:
Droplet Microfluidic Chip Based Nucleic Acid Amplification and Real-Time Detection of Influenza Viruses (2013)

Miniaturized bio-diagnostic devices have the potential to allow for rapid pathogen screening in clinical patient samples, as a low cost and portable alternative to conventional bench-top equipment. Miniaturization of key bio-diagnostic techniques, such as: nucleic acid detection and quantification, polymerase chain reaction (PCR), DNA fingerprinting, enzyme linked immunosorbent assay (ELISA), results in substantial reduction of reaction volumes (expensive samples/reagents) and shorter reaction times. Droplet microfluidics (DMF) is one of several miniaturized bio-sample handling techniques available for manipulating clinical samples and reagents in microliter (10<sup>-6</sup> L) to picoliter (10<sup>-12</sup> L) volume regime. Electro-actuation of sample and reagent in the form of droplets in the aforementioned volume regime, using dielectrophoresis (DEP) and/or Electrowetting (EW) are achieved by means of patterned, insulated metal electrodes on one or more substrates. In this work, we have utilized electro-actuation based DMF technology, integrated with suitably tailored resistive micro-heaters and temperature sensors, to achieve chip based real-time, quantitative PCR (qRT-PCR). This qRT-PCR micro-device was utilized to detect and quantify the presence of influenza A and C virus nucleic acids, using in-vitro synthesized viral RNA segments. The experimental analysis of the DMF micro-device confirms its capabilities in qRT-PCR based detection and quantification of pathogen samples, with accuracy levels comparable to established commercial bench-top equipment (PCR efficiency ∼95%). The limit of detection (LOD) of the chip based qRT-PCR technique was estimated to be ∼5 copies of template RNA per PCR reaction.
PDMA Research on New Product Development Practices: Updating Trends and Benchmarking Best Practices (1997)

Product development professionals may have the feeling that yet another buzzword or magic bullet always lurks just around the corner. However, researchers have devoted considerable effort to helping practioners determine which tools, techniques, and methods really do offer a competitive edge. Starting 30 years ago, research efforts have aimed at understanding NPD practices and identifying those which are deemed “best practices.” During the past five years, pursuit of this goal has produced numerous privately available reports and two research efforts sponsored by the PDMA. Abbie Griffin summarizes the results of research efforts undertaken during the past five years and presents findings from the most recent PDMA survey on NPD best practices. This survey, conducted slightly more than five years after PDMA's first best‐practices survey, updates trends in processes, organizations, and outcomes for NPD in the U.S., and determines which practices are more commonly associated with firms that are more successsful in developing new products. The survey has the following objectives: determining the current status of NPD practices and performance; understanding how product development has changed from five years ago; determining whether NPD practice and performance differ across industry segments; and, investigating process and product development tools that differentiate product development success. The survey findings indicate that NPD processes continue to evolve and become more sophisticated. NPD changes continually on multiple fronts, and firms that fail to keep their NPD practices up to date will suffer an increasingly marked competitive disadvantage. Interestingly, although more than half of the respondents use a cross‐functional stage‐gate process for NPD, more than one‐third of all firms in the study still use no formal process for managing NPD. The findings suggest that firms are not adequately handling the issue of team‐based rewards. Project‐completion dinners are for the most frequently used NPD reward; they are also the only reward used more by best‐practice firms than by the rest of the respondents. The best‐practice firms participating in the study do not use financial rewards for NPD. Compared to the other firms in the study, best‐practice firms use more multifunctional teams, are more likely to measure NPD processes and outcomes, and expect more from their NPD programs.
Product Analysis of GG-Specific Photooxidation of DNA via Electron Transfer:  2-Aminoimidazolone as a Major Guanine Oxidation Product (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTProduct Analysis of GG-Specific Photooxidation of DNA via Electron Transfer: 2-Aminoimidazolone as a Major Guanine Oxidation ProductKatsuhito Kino, Isao Saito, and Hiroshi SugiyamaView Author Information Department of Synthetic Chemistry and Biological Chemistry Faculty of Engineering, Kyoto University, CREST Yoshida, Sakyo, Kyoto 606-8501, Japan Institute for Medical and Dental Engineering Tokyo Medical and Dental University 2-3-10 Kanda Surugadai, Chiyoda, Tokyo 101-0062, Japan Cite this: J. Am. Chem. Soc. 1998, 120, 29, 7373–7374Publication Date (Web):July 29, 1998Publication History Received9 March 1998Published online29 July 1998Published inissue 1 July 1998https://pubs.acs.org/doi/10.1021/ja980763ahttps://doi.org/10.1021/ja980763arapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views647Altmetric-Citations160LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Genetics,High-performance liquid chromatography,Irradiation,Mixtures,Oligomers Get e-Alerts
Investigating Volatility Trends of Silver through an Analysis of Stock Options Prices (2013)

Dylan Houston is a Spring 2013 graduate of Coastal Carolina University's Honors Program with a Bachelor of Science in Information Systems. In addition, he was named the outstanding graduate in information systems. He is involved in the Numbers & Bytes Club as the Vice President and is a member of Upsilon Pi Epsilon (International Honor Society for the Computing and Information Disciplines). He has experience programming with a wide range of technologies and enjoys problem solving and building systems. In his free time he enjoys weightlifting, martial arts, and football. ABSTRACT Volatility is a statistical measure that describes the amount of fluctuation in prices for a given investment; generally, the higher the volatility for an investment, the riskier it is perceived to be. Traders study volatility history so that they can make informed decisions on how to invest capital. The purpose of this article is to analyze implied volatility values, which are derived from the investment's price and are considered the market's estimate of the investment's actual volatility, for silver electronically traded fund (ETF) options in periods of both high and low price movement. In doing so, we desired to see whether or not the general market perception of silver ETF options demonstrated significantly different trends between periods of high and low price movement. Our results demonstrated that implied volatility trends did not show any significant differences between the periods of low and high price movement, and therefore market perception of iShares Silver Trust (SLV) options did not radically change, regardless of the price changes of SLV.
<title>On-line detection of surface defects on steel billets using multiple grazing-incidence light sources</title> (1992)

An optical method for on-line hot steel surface quality inspection is proposed. The method is based on the four laser sources illuminating the surface at grazing incidence from different directions. A CCD line array camera, located perpendicularly over the surface, captures the scattered light generated by each source separately. Surface defects induce shadow and bright field patterns which are related to each source direction and angle of incidence. Such a multiple source configuration is required for the discrimination of real surface defects from normal reflectivity and surface geometry variations. The patterns are digitized for each source separately and then combined using a simple and fast algorithm. The method was tested in our laboratory on cold steel billet samples. Characteristic signatures were obtained for most surface defects such as blisters, scale embedments, large cracks and pinholes. A scanning rate of several thousand lines per second is planned for the industrial prototype by selecting a different wavelength for each laser source and by using a four wavelength CCD line array camera.
Induction of carbohydrate‐specific antibodies in HLA‐DR transgenic mice by a synthetic glycopeptide: a potential anti cancer vaccine for human use (2003)

Over the last few years, anticancer immunotherapy has emerged as a new exciting area for controlling tumors. In particular, vaccination using synthetic tumor-associated antigens (TAA), such as carbohydrate antigens hold promise for generating a specific antitumor response by targeting the immune system to cancer cells. However, development of synthetic vaccines for human use is hampered by the extreme polymorphism of human leukocyte-associated antigens (HLA). In order to stimulate a T-cell dependent anticarbohydrate response, and to bypass the HLA polymorphism of the human population, we designed and synthesized a glycopeptide vaccine containing a cluster of a carbohydrate TAA B-cell epitope (Tn antigen: alpha-GalNAc-Ser) covalently linked to peptides corresponding to the Pan DR 'universal' T-helper epitope (PADRE) and to a cytotoxic T lymphocyte (CTL) epitope from the carcinoembryonic antigen (CEA). The immunogenicity of the construct was evaluated in outbred mice as well as in HLA transgenic mice (HLA-DR1, and HLA-DR4). A strong T-cell dependent antibody response specific for the Tn antigen was elicited in both outbred and HLA transgenic mice. The antibodies induced by the glycopeptide construct efficiently recognized a human tumor cell line underlying the biological relevance of the response. The rational design and synthesis of the glycopeptide construct presented herein, together with its efficacy to induce antibodies specific for native tumor carbohydrate antigens, demonstrate the potential of a such synthetic molecule as an anticancer vaccine candidate for human use.
Identification of new target molecules<i>PTK2</i>,<i>TGFBR2</i>and<i>CD9</i>overexpressed during advanced bone marrow remodelling in primary myelofibrosis (2009)

Primary myelofibrosis (PMF) is a myeloproliferative neoplasm characterized by remodelling of the bone marrow, including progressive myelofibrosis and exaggerated angiogenesis. Advanced PMF frequently shows a full-blown fibre meshwork, which avoids aspiration of cells, and the expression profile of genes related to stroma pathology at this stage remains largely undetermined. We investigated bone marrow core biopsies in PMF showing various degrees of myelofibrosis by custom-made low density arrays (LDA) representing target genes with designated roles in synthesis of extracellular matrix, matrix remodelling, cellular adhesion and motility. Among a set of 11 genes up-regulated in advanced stages of PMF (P < or = 0.01) three candidates, PTK2 protein tyrosine kinase 2 (PTK2), transforming growth factor beta type II receptor (TGFBR2) and motility-related protein-1 (CD9 molecule, CD9), were investigated in more detail. PTK2, TGFBR2 and CD9 were significantly overexpressed in larger series of advanced PMF stages (P < or = 0.01 respectively). Endothelial cells of the increased microvessel network in PMF could be identified as a predominant source for PTK2, TGFBR2 and CD9. CD9 also strongly identified activated fibroblasts in advanced myelofibrosis. We conclude that PTK2, TGFBR2 and CD9 represent new target molecules involved in bone marrow remodelling of PMF and warrant further investigation for potential targeted therapy.
Ontology-based integration of OLAP and information retrieval (2004)

This paper describes an ontology-based approach for building an enterprise knowledge portal that integrates OLAP and information retrieval functionality to access both structured data stored in a data warehouse and unstructured data in form of documents. We discuss how to perform global searches over these information sources. In addition, our approach provides adaptive searching by tracking the user context. When a user performs ad-hoc navigation in an OLAP report, the system will be able to use the query context information to also search for relevant documents.
A New Classification Based on the Kaban's Modification for Surgical Management of Craniofacial Microsomia (2010)

In medicine, classifications are designed to describe accurately and reliably all anatomic and structural components, establish a prognosis, and guide a given treatment. Classifications should be useful in a universal way to facilitate communication between health professionals and to formulate management protocols. In many situations and particularly with craniofacial microsomia, there have been many different classifications that do not achieve this goal. In fact, when there are so many classifications, one can conclude that there is not a clear one that accomplishes all these ends and defines a treatment protocol. It is our intent to present a new classification based on the Pruzansky's classification, later modified by Kaban, to determine treatment protocols based on the degree of osseous deficiency present in the body, ramus, and temporomandibular joint. Different mandibular defects are presented in two patients with craniofacial microsomia type III and IV according to our classification with the corresponding management proposed for each type and adequate functional results.
Silicon nanowire heterostructures for advanced energy and environmental applications: a review (2016)

Semiconductor nanowires (NWs), in particular Si NWs, have attracted much attention in the last decade for their unique electronic properties and potential applications in several emerging areas. With the introduction of heterostructures (HSs) on NWs, new functionalities are obtained and the device performance is improved significantly in many cases. Due to the easy fabrication techniques, excellent optoelectronic properties and compatibility of forming HSs with different inorganic/organic materials, Si NW HSs have been utilized in various configurations and device architectures. Herein, we review the recent developments in Si NW HS-based devices including the fabrication techniques, properties (e.g., light emitting, antireflective, photocatalytic, electrical, photovoltaic, sensing etc) and related emerging applications in energy generation, conversion, storage, and environmental cleaning and monitoring. In particular, recent advances in Si NW HS-based solar photovoltaics, light-emitting devices, thermoelectrics, Li-ion batteries, supercapacitors, hydrogen generation, artificial photosynthesis, photocatalytic degradation of organic dyes in water treatment, chemical and gas sensors, biomolecular sensors for microbial monitoring etc have been addressed in detail. The problems and challenges in utilizing Si NW HSs in device applications and the key parameters to improve the device performance are pointed out. The recent trends in the commercial applications of Si NW HS-based devices and future outlook of the field are presented at the end.
Inheritance of Resistance to Fusarium Crown and Root Rot in Tomato (1987)

Abstract Resistance to Fusarium crown and root rot ( Fusarium oxysporum f. sp. radicis-lycopersici) in the tomato ( Lycopersicon esculentum Mill.) was inherited as a monogenic dominant gene in a cross between the breeding lines 89-1 (resistant) and 1239A (susceptible).
FIBRONECTIN PREVENTS D-galactosamine/Lipopolysaccharide-induced lethal hepatic failure in mice (2005)

Plasma fibronectin (FN) has a broad range of biological functions involved in cellular adhesion, motility, differentiation, apoptosis, hemostasis, wound healing, reticuloendothelial system function, and ischemic injury. In this study, we examined the effects of FN on D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced fulminant liver failure in mice. Female Balb/c mice received intraperitoneal injection of 50 mug/kg of LPS and 400 mg/kg of GalN simultaneously. Thirty minutes before GalN/LPS administration, human plasma FN (FN group) or the same dose of human serum albumin (control group) was given intravenously. GalN/LPS induced a marked decrease in plasma FN, which was reversed by FN pretreatment. The survival rate of the FN group was markedly improved in a dose-dependent manner compared with that of the control group (survival rate 0%). FN prevented increases in the concentrations of serum enzymes and total bilirubin related to liver injury. FN pretreatment significantly suppressed tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-6 levels, and enhanced IL-10 levels in serum and liver tissue compared with the control group. Moreover, TUNEL staining, caspase 3 and 8 activities, and necrosis in the remnant liver were significantly decreased in the FN-treated rats compared with the controls. Furthermore, FN pretreatment inhibited the activation of nuclear factor (NF)-kappaB and increased the expression of Bcl-xL protein in liver tissue. These results suggest that FN protected against GalN/LPS-induced liver failure by a mechanism involving inhibition of NF-kappaB activation, which caused down-regulation of TNF-alpha and involved up-regulation of IL-10, and elevation of Bcl-xL induced a blockage of apoptotic signals, by which apoptosis of hepatocytes caused by GalN/LPS was suppressed.
Functional Expression and Characterization of Trichome-Specific (-)-Limonene Synthase and (+)-α-Pinene Synthase from <i>Cannabis sativa</i> (2007)

Two recombinant, stereospecific monoterpene synthases, a (-)-limonene synthase (CsTPS1) and a (+)-α-pinene synthase (CsTPS2), encoded by Cannabis sativa L. cv. ‘Skunk’ trichome mRNA, have been isolated and characterized. Recombinant CsTPS1 showed a K m value of 6.8 μM, a V max of 1.1 × 10 −4 μmol/min and V max /K m of 0.016; the pH optimum was determined at pH 6.5, and a temperature optimum at 40°C. Recombinant CsTPS2 showed a K m value of 10.5 μM, a V max of 2.2 × 10 −4 μmol/min and V max /K m of 0.021; the pH optimum was determined at pH 7.0, and a temperature optimum at 30°C. Phylogenetic analysis showed that both CsTPSs group within the angiosperms and belong to the Tpsb subgroup of monoterpene synthases. The enzymatic products (-)-limonene and (+)-α-pinene were detected as natural products in C. sativa trichomes.
CYTOKINE RESPONSE TO BURN INJURY (1994)

Plasma levels of interleukin 1 beta (IL-1 beta), tumor necrosis factor alpha (TNF alpha), interleukin 6 (IL-6), and markers of protein metabolism were determined in 12 burn patients throughout the healing period (day 2 to 21 post-injury) to determine the pattern of variations in plasma cytokine concentration. To establish the relationship between cytokine production and the nutritional status a wide range of severity standpoints (burn surface area ranging from 9% to 82%) was chosen. Interleukin 6 levels were increased in all patients throughout the study period; maximum concentrations (615 +/- 198 pg/mL) were reached on day 4 and correlated (p < 0.01) with the extent of burn injury. Tumor necrosis factor alpha levels were also elevated; they were significantly higher on day 7 in the patients who developed sepsis than in the other patients (67 +/- 21 pg/mL vs. 20 +/- 7 pg/mL; p < 0.05) but did not correlate with the extent of burn injury. Interleukin 1 beta was rarely detected. Cortisolemia on day 7 was inversely correlated with levels of TNF alpha but not with those of IL-6. Interleukin 6 levels correlated positively with protein turnover (phenylalaninemia) and catabolism (3-methylhistidine/creatinine ratio) and negatively with levels of fibronectin and transthyretin. Our data indicate that the systemic cytokine response to burn injury is mainly represented by IL-6. These data also support the hypothesis that IL-6 is a key mediator of the variations in protein metabolism following burn injury.
Recent clinical trials evaluating benefit of drug therapy for modification of HDL cholesterol (2013)

Pharmacological manipulation of HDL-C has not improved the cardiovascular outcomes. Several agents have caused harm or unacceptable side-effects. Further studies are needed before one can recommend the use of additional lipid-modifying therapies beyond statins.
Functionalization of nanoparticles for dispersion in polymers and assembly in fluids (2006)

Abstract The fluorescent, magnetic, and conductive properties of nanoparticles can transform polymer‐based materials into composites with higher levels of sophistication than found in polymers alone. The ligand chemistry of nanoparticles is critically important in the development of polymer–nanoparticle composites to prevent nanoparticle aggregation and direct their assembly within polymers. Nanoparticle ligands can also prevent aggregation in solution and direct the assembly of nanoparticles at fluid–fluid interfaces, where interfacial chemistries can be performed to provide new routes to ultrathin composite sheets and capsules. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 5076–5086, 2006
A characterization of broadband user behavior and their e-business activities (2004)

This paper presents a characterization of broadband user behavior from an Internet Service Provider standpoint. Users are broken into two major categories: residential and Small-Office/Home-Office (SOHO). For each user category, the characterization is performed along four criteria: (i) session arrival process, (ii) session duration, (iii) number of bytes transferred within a session and (iv) user request patterns.Our results show that both residential and SOHO session inter-arrival times are exponentially distributed. Whereas residential session arrival rates remain relatively high during the day, SOHO session arrival rates vary much more significantly during the day. On the other hand, a typical SOHO user session is longer and transfers a larger volume of data. Furthermore, our analysis uncovers two main groups of session request patterns within each user category. The first group consists of user sessions that use traditional Internet services, such as e-mail, instant messenger and, mostly, www services. On the other hand, sessions from the second group, a smaller group, use typically peer-to-peer file sharing applications, remain active for longer periods and transfer a large amount of data. Looking further into the e-business services most commonly accessed, we found that subscription-based and advertising services account for the vast majority of user HTTP requests in both residential and SOHO workloads. Understanding these user behavior patterns is important to the development of more efficient applications for broadband users.