gebhart/patrepeval huggingface dataset:
The Effects of the Broadband UVA Radiation on Myeloid Leukemia Cells: The Possible Role of Protein Kinase C in Mediation of UVA‐lnduced Effects (1996)

We examined the effects of broadband UVA radiation (320-400 nm) on a rat myeloid leukemia cell line-chloroma (ChL). A Phillips face tanner model HB 171/A was used as a light source. Chloroma were irradiated through a 5 mm thick glass filter that cut off all of the UVB contamination. The irradiances were measured, from 250 to 400 nm, with a well-characterized and calibrated double-grating spectroradiometer Optronic 742. The overall uncertainty of dose evaluation was estimated to be +/-15% (2 sigma). The cells were irradiated with UVA doses of 4 and 8 J/cm2 and cultured thereafter for 24 h. After this period of time, a marked decline up to 50% was observed in cell proliferation in UVA-irradiated ChL cultures. The cell proliferation decline was found to be caused by simultaneously occurring G2/M phase cell cycle arrest and apoptosis in part of the UVA-irradiated ChL population. Concomitantly, with the decline in cell proliferation, an increase was observed in the expression of the major histocompatibility (MHC) class I and II antigens. Because protein kinase C (PKC) is known to regulate cell proliferation, apoptosis and expression of MHC antigens, and because UVA was shown to regulate PKC activity/expression, we therefore examined whether UVA irradiation has any effect on the expression of isozymes of PKC. Western blots revealed that ChL express alpha, beta I, delta, epsilon, eta, and zeta/iota isozymes of PKC and that expression of all isozymes declined 24 h after UVA irradiation (8 J/cm2). Finally, PKC activation in ChL by exposure to phorbol ester caused cell cycle arrest in G1 phase but did not induce apoptosis. This suggests that the previously shown UVA-induced PKC activation in ChL might be responsible for the induction of MHC antigens but the simultaneously observed ChL apoptosis is likely to be mediated by PKC down-regulation. All together, our results suggest that UVA, at irradiance levels that resemble the outdoor exposure, may have profound effects on the immune-related properties of leukocytes. Thus, we speculate that in vivo the immune functions of leukocytes passing through dermal capillaries might be altered by exposure to solar UVA radiation.
Designing colon‐specific delivery systems for anticancer drug‐loaded nanoparticles: An evaluation of alginate carriers (2013)

Incorporation of drug-loaded nanoparticles (NPs) in colon-specific delivery systems shows potential for raising local drug concentrations, tumor targeting and improving chemotherapy. Alginate microcapsules (15-80 µm diameter) containing insoluble Eudragit(®) RS NPs as models were characterized precisely in terms of NP loading and release kinetics. High NP loading (22%, w/w of the dried microcapsules) combined with negligible release in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) suggested that high concentrations of NPs could be transported to the colon. However, NP aggregation was confirmed at extremely low concentration (0.0003%, w/v) in alginate solution (0.007%, w/v) and after release from alginate microcapsules. Indomethacin, a model anticolorectal cancer drug, was encapsulated in pH-responsive Eudragit(®) S100 NPs (116 nm, 5%, w/w drug loading) using the nanoprecipitation method. Approximately 90% of the drug load was released from the NPs in SGF and SIF before transfer to simulated colon fluid (SCF). However, incorporation of NPs in 2 mm alginate pellets resulted in a significantly higher fraction of the drug load (around 60%) being available for release in SCF. Delivery of isolated NPs to the colon for interaction with and uptake by cancer cells requires elimination of NP-excipient interactions that promote NP aggregation. NP-loaded alginate carriers, meanwhile, offer a promising strategy for delivery of anticancer drugs to tumor sites in the colon and reducing systemic side effects.
A Self-Folding Hydrogel <i>In Vitro</i> Model for Ductal Carcinoma (2016)

A significant challenge in oncology is the need to develop in vitro models that accurately mimic the complex microenvironment within and around normal and diseased tissues. Here, we describe a self-folding approach to create curved hydrogel microstructures that more accurately mimic the geometry of ducts and acini within the mammary glands, as compared to existing three-dimensional block-like models or flat dishes. The microstructures are composed of photopatterned bilayers of poly (ethylene glycol) diacrylate (PEGDA), a hydrogel widely used in tissue engineering. The PEGDA bilayers of dissimilar molecular weights spontaneously curve when released from the underlying substrate due to differential swelling ratios. The photopatterns can be altered via AutoCAD-designed photomasks so that a variety of ductal and acinar mimetic structures can be mass-produced. In addition, by co-polymerizing methacrylated gelatin (methagel) with PEGDA, microstructures with increased cell adherence are synthesized. Biocompatibility and versatility of our approach is highlighted by culturing either SUM159 cells, which were seeded postfabrication, or MDA-MB-231 cells, which were encapsulated in hydrogels; cell viability is verified over 9 and 15 days, respectively. We believe that self-folding processes and associated tubular, curved, and folded constructs like the ones demonstrated here can facilitate the design of more accurate in vitro models for investigating ductal carcinoma.
Analog pixel array detectors (2006)

X-ray pixel array detectors (PADs) are generally thought of as either digital photon counters (DPADs) or X-ray analog-integrating pixel array detectors (APADs). Experiences with APADs, which are especially well suited for X-ray imaging experiments where transient or high instantaneous flux events must be recorded, are reported. The design, characterization and experimental applications of several APAD designs developed at Cornell University are discussed. The simplest design is a ;flash' architecture, wherein successive integrated X-ray images, as short as several hundred nanoseconds in duration, are stored in the detector chips for later off-chip digitization. Radiography experiments using a prototype flash APAD are summarized. Another design has been implemented that combines flash capability with the ability to continuously stream X-ray images at slower (e.g. milliseconds) rates. Progress is described towards radiation-hardened APADs that can be tiled to cover a large area. A mixed-mode PAD, design by combining many of the attractive features of both APADs and DPADs, is also described.
The effect of a leptin single nucleotide polymorphism on quality grade, yield grade, and carcass weight of beef cattle (2005)

Feedlot producers could optimize the value of cattle in a given market grid if they were able to improve the uniformity of the body composition between cattle among loads. Allelic variation due to a single nucleotide transition (cytosine [C] to thymine [T] transition that results in a Arg25Cys) has been demonstrated to be associated with higher leptin mRNA levels in adipose tissue and increased fat deposition in mature beef, but the effect on economically important carcass traits has not been investigated in either market-ready steers or heifers. Therefore, the objective of this study was to determine the effects of a leptin SNP on the quality grade (QG), yield grade (YG), and weight of beef carcasses. A slaughter trial was conducted using 1,435 crossbred finished heifers and 142 crossbred finished steers as they entered the slaughter facility. Canada QG tended (main effect of genotype P = 0.16, but P < 0.01 for both CC vs. TT and CT vs. TT) to be affected by leptin genotype. Specifically, 7.6 and 7.1% more TT carcasses graded Canada AAA or higher than the CT and CC carcasses, respectively, which supports the suggestion that the leptin SNP is associated with carcass fat. The proportion of carcasses grading Canada YG 1, 2, or 3 was affected (P < 0.01, P = 0.05, and P = 0.02 for YG 1, 2, and 3) by leptin genotype. The proportion of TT carcasses of Canada YG 1 was 12.5 and 15.1% lower than that of CT and CC carcasses, respectively, indicating that rearing animals under the same management and feeding system may result in greater carcass fat and a lower probability of the proportion of carcasses grading YG 1 within certain genotypes. The carcass weights of animals with the CC genotype tended (P = 0.07) to be higher than those of the TT genotype (365.5 vs. 362.3 kg). No significant difference was observed between the TT and CT genotypes in carcass weight. The observed associations between leptin genotype and carcass characteristics may represent an opportunity to genetically identify animals that are most likely to reach specific marketing groups.
Transfer of glutamine between astrocytes and neurons (2001)

The export of glutamine from astrocytes, and the uptake of glutamine by neurons, are integral steps in the glutamate-glutamine cycle, a major pathway for the replenishment of neuronal glutamate. We review here the functional and molecular identification of the transporters that mediate this transfer. The emerging picture of glutamine transfer in adult brain is of a dominant pathway mediated by system N transport (SN1) in astrocytes and system A transport (SAT/ATA) in neurons. The participating glutamine transporters are functionally and structurally related, sharing the following properties: (a) unlike many neutral amino acid transporters which have proven to be obligate exchangers, these glutamine transporters mediate net substrate transfer energized by coupling to ionic gradients; (b) they are sensitive to small pH changes in the physiological range; (c) they are susceptible to adaptive and humoral regulation; (d) they are related structurally to the AAAP (amino acid and auxin permeases) family of transporters. A key difference between SN1 and the SAT/ATA transporters is the ready reversibility of glutamine fluxes via SN1 under physiological conditions, which allows SN1 both to sustain a glutamine concentration gradient in astrocytes and to mediate the net outward flux of glutamine. It is likely that the ASCT2 transporter, an obligate exchanger of neutral amino acids, displaces the SN1 transporter as the main carrier of glutamine export in proliferating astrocytes.
The Design of a Controller for a Following Vehicle in an Emergency Lane Change Maneuver (1999)

A lane change maneuver is one of the many appropriate responses to an emergency situation. The design of an Emergency Lane Change (ELC) maneu- ver for a platoon of two vehicles is considered in this paper. In response to the presence of a stationary or a slowly moving obstacle in the lane or any such emergency, the lead vehicle in the platoon designs an ELC trajectory on-line and broadcasts the trajectory curvature information in real time to its follower. The problem considered, in this paper, is the design of an integrated lateral and longitudinal controller that enables the follower to track the lead vehicle's trajectory, while maintaining a desired following distance. A sliding mode con- troller is developed for this purpose and its performance is demonstrated in simulations.
An Efficient Robust Eye Localization by Learning the Convolution Distribution Using Eye Template (2015)

Eye localization is a fundamental process in many facial analyses. In practical use, it is often challenged by illumination, head pose, facial expression, occlusion, and other factors. It remains great difficulty to achieve high accuracy with short prediction time and low training cost at the same time. This paper presents a novel eye localization approach which explores only one-layer convolution map by eye template using a BP network. Results showed that the proposed method is robust to handle many difficult situations. In experiments, accuracy of 98% and 96%, respectively, on the BioID and LFPW test sets could be achieved in 10 fps prediction rate with only 15-minute training cost. In comparison with other robust models, the proposed method could obtain similar best results with greatly reduced training time and high prediction speed.
TDRSS telecommunications payload - An overview (1977)

The telecommunications payload is described for the tracking and data relay satellite system (TDRSS). The salient design features of the electronic equipment, as well as the performance requirements that such equipment must satisfy to fulfill NASA and Western Union communication functions are presented. Operational characteristics associated with the single and multiple access channels used to relay information to and from users are also highlighted.
Mechanisms of Histone Deacetylase Inhibitor-Regulated Gene Expression in Cancer Cells (2014)

Understanding the mechanisms by which HDACi regulate gene expression and an appreciation of their transcriptional targets could facilitate the ongoing clinical development of these emerging therapeutics. In particular, this knowledge could inform the design of rational drug combinations involving HDACi, and facilitate the identification of mechanism-based biomarkers of response.
Local control of uniaxial anisotropy in LPE bubble garnet films (1974)

Local control of the growth-induced anisotropy in LPE bubble garnet films can be obtained by masking part of the film with a layer of epitaxial Gd3Ga5O12 and annealing at elevated temperatures (∼ 1200 °C) in a nitrogen atmosphere for approximately 1 h. In the regions exposed to nitrogen, the introduction of oxygen vacancies accelerates the reduction of Ku by the randomizing of the rare-earth ion distribution.
The Preparation and Professionalization of Economics Instructors in Two-Year Colleges (1970)

With the rapid growth of two-year colleges and the probability that an increasing proportion of students will get their introductory economics in these institutions comes a natural concern over the quality of instruction found therein. Lewis reviews three surveys dealing with the preparation of junior college teachers of economics, offers explanations for the dearth of well-trained economists in the two-year colleges, and suggests ways of meeting the special needs of junior college teachers. He calls for both pre-service and in-service training programs designed to upgrade the teaching of economics in the two-year colleges.
Identification of Magnolol and Honokiol as Anxiolytic Agents in Extracts of Saiboku-to, an Oriental Herbal Medicine (1998)

The principal active anxiolytic components in Saiboku-to, an Oriental herbal medicine, have been isolated and identified as magnolol (5,5'-di-2-propenyl-1,1'-biphenyl-2,2'-diol) and honokiol (3',5-di-2-propenyl-1,1'-biphenyl-2,4'-diol). Evaluation by means of an elevated plus-maze test showed that honokiol was at least 5000 times more potent than Saiboku-to when mice were treated orally for 7 days.
An energy-efficient noise-tolerant dynamic circuit technique (2000)

Noise in deep submicron technology combined with the move toward dynamic circuit techniques have raised concerns about reliability and energy efficiency of VLSI systems in the deep submicron era. To address this problem, a new noise-tolerant dynamic circuit technique is presented. The average noise threshold energy (ANTE) and the energy normalized ANTE (NANTE) metrics are proposed to quantify the noise immunity and energy efficiency, respectively. Simulation results in 0.35-/spl mu/m CMOS for NAND gate and full-adder designs indicate that the proposed technique improves the ANTE and NANTE by 2/spl times/ and 1.4/spl times/ over conventional domino circuits. The improvement in the NANTE is 11% higher than the existing noise-tolerance techniques. Furthermore, the proposed technique has a smaller area overhead (36%) as compared to static circuits whose area overhead is 60%. Also presented in this paper is an ASIC developed in 0.35-/spl mu/m CMOS to evaluate the performance of the proposed technique. Experimental results demonstrate a 27% average improvement in noise immunity over conventional dynamic circuits.
Assessment of inflammation and fibrosis in non‐alcoholic fatty liver disease by imaging‐based techniques (2012)

Non-alcoholic fatty liver disease (NALFD) is a burgeoning global health problem, and the assessment of disease severity remains a clinical challenge. Conventional imaging and clinical blood tests are frequently unable to determine disease activity (the degree of inflammatory change) and fibrotic severity, while the applicability of histological examination of liver biopsy is limited. Imaging platforms provide liver-specific structural information, while newer applications of these technologies non-invasively exploit the physical and chemical characteristics of liver tissue in health and disease. In this review, conventional and newer imaging-based techniques for the assessment of inflammation and fibrosis in NAFLD are discussed in terms of diagnostic accuracy, radio-pathological correlations, and practical considerations. In particular, recent clinical studies of ultrasound (US)-based and magnetic resonance elastography techniques are evaluated, while the potential of contrast-enhanced US and magnetic resonance spectroscopy techniques is discussed. The development and application of these techniques is starting to reduce the clinical need for liver biopsy, to produce surrogate end-points for interventional and observational clinical studies, and through this, to provide new insights into the natural history of NAFLD.
Spectral tuning and near-field imaging of photonic crystal microcavities (2008)

We experimentally observe a sizable and reversible spectral tuning of the resonances of a two-dimensional photonic crystal microcavity induced by the introduction of a subwavelength size glass tip. The comparison between experimental near-field data, collected with $\ensuremath{\lambda}∕6$ spatial resolution, and results of numerical calculations shows that the spectral shift induced by the tip is proportional to the local electric field intensity of the cavity mode. This observation proves that the electromagnetic local density of states in a microcavity can be directly measured by mapping the tip-induced spectral shift with a scanning near-field optical microscope.
Use fluid bed reactor for maleic anhydride from butane (1985)

A new process is described that incorporates three major improvements over the conventional air oxidation of benzene in a fixed-bed reactor system. The new ALMA Process was developed jointly by Alusuisse Italia and Lummus Crest. It includes the following process improvements: n-Butane feedstock, fluidized-bed reactor system, and a continuous maleic anhydride recovery system using an organic solvent. A summary of the process is given, as well as the steps in its development and its economic advantages.
An Assessment of Thermodynamic Consistency Tests for Vapor-Liquid Equilibrium Data (1997)

Abstract Consistency tests are techniques that allow, in principle, the assessment of experimental vapor-liquid equilibrium data on the basis of the Gibbs-Duhem equation. Much empiricism and arbitrariness is frequently observed in the analysis and application of consistency tests, a situation that may question their usefulness. Perfect data satisfy exactly the Gibbs-Duhem relation, but acceptable data obey it within a tolerable limits (consistency criteria) which does not give a unique answer, regarding the quality of the data, when different consistency procedures are used. As with any approximation, application of consistency tests requires rigour, reasonable assumptions and models, and an examination of the data and results of the test. In this work, a set of guidelines that allow a critical interpretation of consistency analysis is proposed and special attention is given to the point-to-point test which is extensively used today in data evaluation.
A Blockchain-Based Privacy-Preserving Payment Mechanism for Vehicle-to-Grid Networks (2018)

As an integral part of V2G networks, EVs receive electricity from not only the grid but also other EVs and may frequently feed the power back to the grid. Payment records in V2G networks are useful for extracting user behaviors and facilitating decision-making for optimized power supply, scheduling, pricing, and consumption. Sharing payment and user information, however, raises serious privacy concerns in addition to the existing challenge of secure and reliable transaction processing. In this article, we propose a blockchain-based privacy preserving payment mechanism for V2G networks, which enables data sharing while securing sensitive user information. The mechanism introduces a registration and data maintenance process that is based on a blockchain technique, which ensures the anonymity of user payment data while enabling payment auditing by privileged users. Our design is implemented based on Hyperledger to carefully evaluate its feasibility and effectiveness.
Reduction of Combustion Irreversibility in a Gas Turbine Power Plant Through Off-Gas Recycling (1995)

Combustion in conventional fossil-fueled power plants is highly irreversible, resulting in poor overall energy conversion efficiency values (less than 40 percent in many cases). The objective of this paper is to discuss means by which this combustion irreversibility might be reduced in gas turbine power cycles, and the conversion efficiency thus improved upon. One such means is thermochemical recuperation of exhaust heat. The proposed cycle recycles part of the exhaust gases, then mixes them with fuel prior to injection into a reformer. The heat required for the endothermic reforming reactions is provided by the hot turbine exhaust gases. Assuming state-of-the-art technology, and making a number of simplifying assumptions, an overall efficiency of 65.4 percent was attained for the cycle, based on the lower heating value (LHV) of the methane fuel. The proposed cycle is compared to a Humid Air Turbine (HAT) cycle with similar features that achieves an overall efficiency of 64.0 percent. The gain in cycle efficiency that can be attributed to the improved fuel oxidation process is 1.4 percentage points. Compared to current high-efficiency gas turbine cycles, the high efficiency of both cycles studied therefore results mainly from the use of staged compression and expansion with intermediate cooling and reheating, respectively.
Insulin-mediated inhibition of apolipoprotein B secretion requires an intracellular trafficking event and phosphatidylinositol 3-kinase activation: studies with brefeldin A and wortmannin in primary cultures of rat hepatocytes (1996)

Insulin inhibition of the secretion of apolipoprotein B (apo B) was studied in primary cultures of rat hepatocytes by using brefeldin A (BFA), an inhibitor of protein transport from the endoplasmic reticulum (ER) to the Golgi apparatus, and by using the phosphatidylinositol 3-kinase (PI 3-K) inhibitor wortmannin. Incubation of hepatocytes with BFA (10 micrograms/ml) for 1 h inhibited the subsequent secretion of apo B, albumin and transferrin for up to 3 h. BFA treatment resulted in the time-dependent accumulation in cells of [14C]leucine-labelled proteins and apo B. Under conditions where insulin decreased total apo B (cell plus secreted), BFA blocked the insulin-dependent effect. These results suggest that export of apo B from the ER is a prerequisite for the observed insulin effect. Treatment of hepatocytes with wortmannin for 20 min abolished insulin inhibition of apo B secretion, suggesting that the insulin effect on the apo B pathway involves activation of PI 3-K. Enzyme inhibitor studies indicate that chymostatin and (+)-(2S,3S)-3-[(S)-methyl-1-(3-methylbutylcarbamoyl)-butylcarba moyl]-2- oxiranecarboxylate (E-64-c) partially block insulin effects on apo B compared with leupeptin, which had no discernible effect. The cell-permeable derivative of E-64-c, EST, and N-Ac-Leu-Leu-norleucinal (ALLN) were most effective in blocking insulin effects on apo B. These results suggest that insulin action on apo B in primary rat hepatocytes involves (1) vesicular movement of apo B from the ER; (2) activation of PI 3-K and (3) a cellular protease that is either a cysteine- or calcium-activated neutral protease.