gebhart/patrepeval huggingface dataset:
Immobilization of immunoglobulins on silica surfaces. Stability (1985)

The development of new immunosensors based on surface-concentration-measuring devices requires a stable and reproducible immobilization of antibodies on well-characterized solid surfaces. We here report on the immobilization of immunoglobulin G (IgG) on chemically modified silica surfaces. Such surfaces may be used in various surface-oriented analytical methods. Reactive groups were introduced to the silica surfaces by chemical-vapour deposition of silane. The surfaces were characterized by ellipsometry, contact-angle measurements and scanning electron microscopy. IgG covalently bound by the use of thiol-disulphide exchange reactions, thereby controlling the maximum number of covalent bonds to the surface, was compared with IgG adsorbed on various silica surfaces. This comparison showed that the covalently bound IgG has a superior stability when the pH was lowered or incubation with detergents, urea or ethylene glycol was carried out. The result was evaluated by ellipsometry, an optical technique that renders possible the quantification of amounts of immobilized IgG. The results outline the possibilities of obtaining a controlled covalent binding of biomolecules to solid surfaces with an optimal stability and biological activity of the immobilized molecules.
Weighted vote for trees aggregation in Random Forest (2014)

Random Forest RF is a successful technique of ensemble prediction that uses the majority voting or an average depending on the combination. However, it is clear that each tree in a random forest can have different contribution to the treatment of some instance. In this paper, we show that the prediction performance of RF's can still be improved by replacing the GINI index with another index (twoing or deviance). Our experiments also indicate that weighted voting gives better results compared to the majority vote.
Synthesis and Cytotoxic Evaluation of Combretafurans, Potential Scaffolds for Dual-Action Antitumoral Agents (2006)

We have synthesized rigid analogues of combretastatin bearing a furan ring in place of the olefinic bridge. These compounds are cytotoxic at nanomolar concentrations in neuroblastoma cells, display a similar structure-activity relationship compared to combretastatin A4, and inhibit tubulin polymerization. We also show that the furan ring can be further functionalized. Thus, it is possible that combretafurans could act as scaffolds for the development of dual-action antitumoral agents.
Immunological studies of human placentae: identification and distribution of proteins in mature chorionic villi. (1977)

Cryostat sections of fifteen full-term, normal, human placentae have been studied by indirect immunofluorescence, using characterized antisera to a large number of proteins, in order to localize particular protein antigens to well-defined anatomical regions. Actin, plasminogen, and transferrin were identified in trophoblasts. Beta-2-microglobulin was uniformly absent from these cells. The complement component C3, the immunoglobulin IgG, and fibrinogen and collagen were identified in trophoblastic basement membranes. Of these IgG was the most sparingly represented, and C3 was not found on all trophoblastic basement membranes. Many proteins were identified within the stroma of chorionic billi, collagen being the most abundant. Many of these persisted following prolonged washing of the tissues. All four IgG subclasses were present in stroma, IgG1 and IgG3 being most prominent. Stromal cells were positive for beta-2-microglobulin, suggesting a unique sequestration of this protein on stromal cells but not on trophoblasts. Placental stem vessels were surrounded by collagen, and the walls of these vessels stained brightly for myosin: the vascular endothelium contained beta-2-microglobulin and actin. Peri- and inter-villous fibrin were faintly positive for several proteins, but these areas stained intensely for fibrinogen, plasminogen, alpha-2-macroglobulin and C4. This approach has proven to be useful in establishing precise definitions of the molecular morphology of normal human placentae.
Preventing Capability Leaks in Secure JavaScript Subsets. (2010)

Publishers wish to sandbox third-party advertisements to protect themselves from malicious advertisements. One promising approach, used by ADsafe, Dojo Secure, and Jacaranda, sandboxes advertisements by statically verifying that their JavaScript conforms to a safe subset of the language. These systems blacklist known dangerous properties that would let advertisements escape the sandbox. Unfortunately, this approach does not prevent advertisements from accessing new methods added to the built-in prototype objects by the hosting page. In this paper, we show that onethird of the Alexa US Top 100 web sites would be exploitable by an ADsafe-verified advertisement. We propose an improved statically verified JavaScript subset that whitelists known-safe properties using namespaces. Our approach maintains the expressiveness and performance of static verification while improving security. 1
Numerical model of multiple-Raman-shifting excimer lasers to the blue-green in H_2 (1981)

Results of a computer model, which describes all resonant parametric and Raman processes involving frequencies up to fifth-order Stokes waves in a multiple-Raman-shifting amplifier using H(2), are reported. The model is primarily concerned with downconverting ultraviolet excimer laser wavelengths to the blue-green. Results for shifting the XeCl laser (308 nm) to the third Stokes wave (499 nm) are presented.
Photonic Phase Gate via an Exchange of Fermionic Spin Waves in a Spin Chain (2010)

We propose a new protocol for implementing the two-qubit photonic phase gate. In our approach, the π phase is acquired by mapping two single photons into atomic excitations with fermionic character and exchanging their positions. The fermionic excitations are realized as spin waves in a spin chain, while photon storage techniques provide the interface between the photons and the spin waves. Possible imperfections and experimental systems suitable for implementing the gate are discussed.
Mechanism analysis of the capacitance contributions and ultralong cycling-stability of the isomorphous MnO<sub>2</sub>@MnO<sub>2</sub> core/shell nanostructures for supercapacitors (2015)

The isomorphous MnO<sub>2</sub>@MnO<sub>2</sub> core/shell nanostructures synthesized through a facile hydrothermal process showed remarkable electrochemical performance, <italic>i.e.</italic>, a high specific capacitance with excellent cycling stability.
Maintenance of 2,3-DPG and ATP Levels in Blood Storedfor 30 Days at a Constant pH Obtained by Variation of C0(2) (1978)

A novel procedure for storing blood at a controlled pH consists in collecting blood in a pH 8.20, Tris-CPD solution and storing it in a special recipient including a gas permeable membrane under a CO(2) atmosphere. The recipient is placed in an atmosphere of variable CO(2)content, so that the initial alkalinity of the preservative is balanced by dissolved CO(2), the proportion of which is diminished when lactate production increases with storage. 2,3-DPG and ATP were studied at three different pH levels of approximately 7.25, 7.45, and 7.65 at 4°C. The best pH for the simultaneous maintenance of 2,3-DPG and ATP was 7.65. Under these conditions, 2,3-DPG is maintained at its initial level and ATP at 55% of its initial level at the 30th day. Lactate production is linear and hemolysis moderate.
Selected physiological responses of kudzu to different chromium concentrations (2001)

This study was designed to evaluate the potential use of kudzu (Pueraria montana var. lobata) as a phytoremediator to reduce Cr (VI) contamination. In addition, the effect of Cr concentrations of 4, 8, and 12 mg L –1 on kudzu growth, gas exchange, pigment concentration, and carbohydrate accumulation were evaluated. In general, Cr uptake by kudzu was increased as Cr concentration increased in the growing media. Most of the Cr taken up by kudzu was accumulated in the root as indicated by the significantly higher ratio of root/shoot Cr concentrations at increasing Cr levels in the root environment. Kudzu growth, photosynthetic rate, and chlorophyll a and b concentrations declined as the Cr concentration increased. The decrease in photosynthesis was independent of the internal carbon dioxide. Anthocyanin was found to increase as Cr concentration gradually increased from 4 to 12 mg L –1 . Kudzu clearly demonstrated its ability to survive at Cr concentrations up to 8 mg L –1 in the root environment. Kudzu, therefore, can be considered as a replacement crop in areas of Cr contamination falling within the concentration range of this study. Key words: Carbohydrate, chromium uptake, photosynthesis, phytoremediation
Nonlinear L¥ Identifications for CH4-Air Combustion (1993)

A numerical scheme is proposed to predict complex chemical reactions aiming to obtain the moles of chemical products, reaction temperature and mixture density as a result of complex chemical reaction. First, by treating the calculation of complex chemical reaction as a typical identification problem, a nonlinear L, minimization technique can be used to predict the moles of dominant species in the chemical reaction by taking into account the conservation of energy, atoms, and ions in the reaction. Lastly, the predicted results are treated as the accurate set of initial guess for a numerical gradient method to extract the exact thermodynamic states or the state relationships of chemical reaction. The calculated state relationships are then employed to analyse the structure of turbulent diffusion flame by injecting methane gas at 300 OK into open air at 700 OK.
The Role of Return Value Prediction in Exploiting Speculative Method- Level Parallelism (2003)

This work studies the performance impact of return value prediction in a system that supports speculative method-level parallelism (SMLP). A SMLP system creates a speculative thread at each method call. This allows the method and the code from which it is called to be executed in parallel. To improve performance, the return values of methods are predicted in hardware so that no method has to wait for its sub-method to complete before continuing to execute. We find that two-thirds of return values need to be predicted, and perfect return value prediction improves performance by an average of 44% over no return value prediction. However, the performance of realistic predictors is limited by poor prediction accuracy on integer return values and unfavorable SMLP conditions. A new Parameter Stride (PS) predictor is proposed to overcome the deficiencies of the standard predictors by predicting based on method arguments. Combining the PS predictor with previous predictors results in an average 7% speedup versus a system with hybrid return value prediction and 21% speedup versus no return value prediction.
Final Report on the Safety Assessment of PEG-6,-8, and-20 Sorbitan Beeswax (2001)

Polyethylene Glycol (PEG)-6,-8, and-20 Sorbitan Beeswax are ethoxylated derivatives of Beeswax that function as surfactants in cosmetic formulations. Only PEG-20 Sorbitan Beeswax is currently reported to be used, at concentrations up to 11%. Few data on the PEGs Sorbitan Beeswax ingredients were available. This safety assessment relied upon the available data from previous safety assessments of Beeswax, Synthetic Beeswax, Sorbitan Esters, PEGs, and PEG Sorbitan fatty acid esters, also known as Polysorbates. The ester linkage of PEG Sorbitan fatty acid esters was hydrolyzed after oral administration, and the PEG Sorbitan moiety was poorly absorbed from the gastrointestinal tract. Sorbitan Stearate was hydrolyzed to stearic acid and anhydrides of sorbitol in the rat. PEGs are readily absorbed through damaged skin and are associated with contact dermatitis and systemic toxicity in burn patients. PEGs were not sensitizing to normal skin. PEGs did not cause reproductive toxicity, nor were tested PEGs mutagenic or carcinogenic. Sorbitol was not a reproductive or developmental toxin in multigenerational studies in rats. Neither Beeswax nor Synthetic Beeswax produced significant acute animal toxicity, ocular irritation, skin irritation, or skin sensitization. Polysorbates produced no acute or long-term effects, were generally not irritating or sensitizing, and were noncarcinogenic, although studies did demonstrate enhancement of the activity of chemical carcinogens. Sorbitan fatty acid esters were relatively nontoxic via ingestion, generally were not skin irritants or sensitizers, and were not mutagenic or carcinogenic. Sorbitan Laurate was a cocarcinogen in a mouse skin-painting study. PEG-6 Sorbitan Beeswax delivered via a stomach tube was nontoxic in rats in acute studies. Undiluted PEG-6 Sorbitan Beeswax was nonirritating to the eyes of rabbits and was non-irritating to intact and abraded skin of rabbits. PEG-20 Sorbitan Beeswax was only minimally irritating to rabbit eyes at concentrations as high as 30%, and was not a significant skin irritant in rabbits exposed to a product with PEG-20 Sorbitan Beeswax at 2%. In clinical tests, PEG-6 and-20 Sorbitan Beeswax at concentrations up to 3% were only minimally irritating and were nonsensitizers. Careful consideration was made of the data on the cocarcinogenesis, but the high exposure levels, high frequency of exposure, and absence of a dose-response led to the conclusion that there was not a cocarcinogenesis risk with the use of these ingredients in cosmetic formulations. Accordingly, these ingredients were considered safe for use in cosmetic formulations under the present practices of use.
Navigator GPS Receiver for Fast Acquisition and Weak Signal Space Applications (2004)

NASA Goddard Space Flight Center (GSFC) is developing a new space-borne GPS receiver that can operate effectively in the full range of Earth orbiting missions from Low Earth Orbit (LEO) to geostationary and beyond. Navigator is designed to be a fully space flight qualified GPS receiver optimized for fast signal acquisition and weak signal tracking. The fast acquisition capabilities provide exceptional time to first fix performance (TIFF) with no a priori receiver state or GPS almanac information, even in the presence of high Doppler shifts present in LEO (or near perigee in highly eccentric orbits). The fast acquisition capability also makes it feasible to implement extended correlation intervals and therefore significantly reduce Navigator s acquisition threshold. This greatly improves GPS observability when the receiver is above the GPS constellation (and satellites must be tracked from the opposite side of the Earth) by providing at least 10 dB of increased acquisition sensitivity. Fast acquisition and weak signal tracking algorithms have been implemented and validated on a hardware development board. A fully functional version of the receiver, employing most of the flight parts, with integrated navigation software is expected by mid 2005. An ultimate goal of this project is to license the Navigator design to an industry partner who will then market the receiver as a commercial product.
Monolithically Integrated 100 GHz DFB-TWEAM (2009)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> A monolithically integrated distributed feedback (DFB) laser and traveling-wave electro-absorption modulator (TWEAM) with <formula formulatype="inline"><tex Notation="TeX">${\geq} 100$</tex></formula> GHz <formula formulatype="inline"> <tex Notation="TeX">${-}3$</tex></formula> dBe bandwidth suitable for Non-return-to-zero (NRZ) operation with on-off keying (OOK) is presented. The steady-state, small-signal modulation response, microwave reflection, chirp characteristic, and both data operation and transmission were investigated. The DFB-TWEAM was found to be an attractive candidate for future short distance communication in high bitrates systems. </para>
Serum Antioxidant Activity in Uremic Patients (1985)

Serum antioxidant activity (AOA) was examined in 35 healthy subjects and 111 patients with chronic renal failure (CRF), consisting of 13 patients in the predialysis stage, 11 requiring the start of regular dialysis therapy (RDT) and 87 undergoing RDT. Serum AOA was determined by assaying serum activity to inhibit malondialdehyde (MDA) generation. AOA levels were significantly lower in CRF patients, and the lowest levels were noticed in patients with uremic symptoms requiring the start of RDT. These levels were restored to a subnormal level during RDT. Defective serum AOA appears to be an endogenous metabolic consequence in uremia. Sera with low AOA tended to show high MDA levels, indicating that patients with low serum AOA were susceptible to cellular injury by lipid peroxidation. It is proposed that defective serum AOA may contribute to a certain uremic toxicity through peroxidative cell damage.
Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle (2015)

Loss of gamma-sarcoglycan (γ-SG) induces muscle degeneration and signaling defects in response to mechanical load, and its absence is common to both Duchenne and limb girdle muscular dystrophies. Growing evidence suggests that aberrant signaling contributes to the disease pathology; however, the mechanisms of γ-SG-mediated mechanical signaling are poorly understood. To uncover γ-SG signaling pathway components, we performed yeast two-hybrid screens and identified the muscle-specific protein archvillin as a γ-SG and dystrophin interacting protein. Archvillin protein and message levels were significantly upregulated at the sarcolemma of murine γ-SG-null (gsg(-/-)) muscle but delocalized in dystrophin-deficient mdx muscle. Similar elevation of archvillin protein was observed in human quadriceps muscle lacking γ-SG. Reintroduction of γ-SG in gsg(-/-) muscle by rAAV injection restored archvillin levels to that of control C57 muscle. In situ eccentric contraction of tibialis anterior (TA) muscles from C57 mice caused ERK1/2 phosphorylation, nuclear activation of P-ERK1/2 and stimulus-dependent archvillin association with P-ERK1/2. In contrast, TA muscles from gsg(-/-) and mdx mice exhibited heightened P-ERK1/2 and increased nuclear P-ERK1/2 localization following eccentric contractions, but the archvillin-P-ERK1/2 association was completely ablated. These results position archvillin as a mechanically sensitive component of the dystrophin complex and demonstrate that signaling defects caused by loss of γ-SG occur both at the sarcolemma and in the nucleus.
Active-Materials Induced-Strain Actuation for Aeroelastic Vibration Control (2000)

Recent achievements in the application of active- materials induced-strain actuation to counteract aeroelastic and vibration effects in helicopters and fixed wing aircraft are reviewed. First, the induced-strain actuation principles and capabilities are briefly presented. Next, the attention is focused on the smart rotor blade applications. Induced twist, active blade tip, and active blade flap are presented, with emphasis on experimental results. Then, fixed wing aircraft applications are considered. Experiments of active flutter control, buffet suppression, gust load alleviation, and sonic fatigue reduction are discussed. Conclusions and directions for further work are presented.
A Distinct Subset of Proinflammatory Neutrophils Isolated from Patients with Systemic Lupus Erythematosus Induces Vascular Damage and Synthesizes Type I IFNs (2010)

Neutrophil-specific genes are abundant in PBMC microarrays from lupus patients because of the presence of low-density granulocytes (LDGs) in mononuclear cell fractions. The functionality and pathogenicity of these LDGs have not been characterized. We developed a technique to purify LDGs from lupus PBMCs and assessed their phenotype, function, and potential role in disease pathogenesis. LDGs, their autologous lupus neutrophils, and healthy control neutrophils were compared with regard to their microbicidal and phagocytic capacities, generation of reactive oxygen species, activation status, inflammatory cytokine profile, and type I IFN expression and signatures. The capacity of LDGs to kill endothelial cells and their antiangiogenic potential were also assessed. LDGs display an activated phenotype, secrete increased levels of type I IFNs, TNF-alpha, and IFN-gamma, but show impaired phagocytic potential. LDGs induce significant endothelial cell cytotoxicity and synthesize sufficient levels of type I IFNs to disrupt the capacity of endothelial progenitor cells to differentiate into mature endothelial cells. LDG depletion restores the functional capacity of endothelial progenitor cells. We conclude that lupus LDGs are proinflammatory and display pathogenic features, including the capacity to synthesize type I IFNs. They may play an important dual role in premature cardiovascular disease development in systemic lupus erythematosus by simultaneously mediating enhanced vascular damage and inhibiting vascular repair.
Occlusion, Orthodontic treatment, and temporomandibular disorders: a review. (1995)

A review of the current literature regarding the interaction of morphologic and functional occlusal factors relative to TMD indicates that there is a relatively low association of occlusal factors in characterizing TMD. Skeletal anterior open bite, overjets greater than 6 to 7 mm, retruded cuspal position/intercuspal position slides greater than 4 mm, unilateral lingual crossbite, and five or more missing posterior teeth are the five occlusal features that have been associated with specific diagnostic groups of TMD conditions. The first three factors often are associated with TMJ arthropathies and may be the result of osseous or ligamentous changes within the temporomandibular articulation. With regard to the relationship of orthodontic treatment to TMD, the current literature indicates that orthodontic treatment performed during adolescence generally does not increase or decrease the odds of developing TMD later in life. There is no elevated risk of TMD associated with any particular type of orthodontic mechanics or with extraction protocols. Although a stable occlusion is a reasonable orthodontic treatment goal, not achieving a specific gnathologically ideal occlusion does not result in TMD signs and symptoms. Thus, according to the existing literature, the relationship of TMD to occlusion and orthodontic treatment is minor. Signs and symptoms of TMD occur in healthy individuals and increase with age, particularly during adolescence; thus, TM disorders that originate during various types of dental treatment may not be related to the treatment but may be a naturally occurring phenomenon.
Total protein extraction from cultured cells for use in electrophoresis and western blotting. (1996)

Experimentation with cultured cells often requires analyzing cellular protein extract by gel electrophoresis and immunoblotting. Traditional methods for extracting cellular proteins by homogenization or detergent solubilization usually produce protein samples that are viscous (due to the presence of DNA) and prone to degradation due to the presence of endogenous protease activity. We have developed a method that involves solubilization of cells with sodium dodecyl sulfate (SDS), precipitation of proteins with trichloroacetic acid (TCA) with special physical exclusion of DNA aggregate and reconstitution of precipitated proteins with Tris base. Protein samples prepared by this method contain little DNA, making them ideal for long-term storage. The solubilized total protein extracts are fully compatible with protein assay, gel electrophoresis and Western blotting. When compared to protein extracts from a homogenization method, those from the TCA method showed an identical total protein staining pattern on SDS polyacrylamide gel electrophoresis and contained distinct cellular proteins recognized by many monoclonal and polyclonal antibodies tested (including anti-actin, spectrin, protein kinase C (alpha), talin and spectrin) on Western blots.