gebhart/patrepeval huggingface dataset:
Retinoic Acid Signaling Restricts the Cardiac Progenitor Pool (2005)

Organogenesis begins with specification of a progenitor cell population, the size of which provides a foundation for the organ's final dimensions. Here, we present a new mechanism for regulating the number of progenitor cells by limiting their density within a competent region. We demonstrate that retinoic acid signaling restricts cardiac specification in the zebrafish embryo. Reduction of retinoic acid signaling causes formation of an excess of cardiomyocytes, via fate transformations that increase cardiac progenitor density within a multipotential zone. Thus, retinoic acid signaling creates a balance between cardiac and noncardiac identities, thereby refining the dimensions of the cardiac progenitor pool.
Fiber-Optic System for Dual-Modality Imaging of Glucose Probes 18F-FDG and 6-NBDG in Atherosclerotic Plaques (2014)

This novel fiber-optic imaging system was sensitive to both radionuclide and fluorescent glucose probes taken up by murine atherosclerotic plaques. In addition, 6-NBDG is a promising novel fluorescent probe for detecting macrophage-rich atherosclerotic plaques.
Accelerated ray-casting for curvilinear volumes (1998)

We present an efficient and robust ray-casting algorithm for directly rendering a curvilinear volume of arbitrarily-shaped cells. We designed the algorithm to alleviate the consumption of CPU power and memory space. By incorporating the essence of the projection paradigm into the ray-casting process, we have successfully accelerated the ray traversal through the grid and data interpolations at sample points. Our algorithm also overcomes the conventional limitation requiring the cells to be convex. Application of this algorithm to several commonly-used curvilinear data sets has produced a favorable performance when compared with recently reported algorithms.
Production of Cuticle-degrading Enzymes by the Entomopathogen Metarhizium Anisopliae during Infection of Cuticles from Calliphora Vomitoria and Manduca Sexta (1987)

SUMMARY: A biochemical and histochemical investigation with specific substrates and inhibitors was used to visualize protease, esterase and aminopeptidase activities produced in situ during penetration of Calliphora vomitoria and Manduca sexta cuticles by hyphae of the entomopathogenic fungus Metarhizium anisopliae. Two endoproteases, and aminopeptidase and esterase activities, were mainly localized in simple and complex appressoria and germinating conidia. The effect of inhibitors on two characterized proteases (Pr1 and Pr2) and aminopeptidase activity in appressorial plates was quantified by microdensitometric measurement of reaction products. Pr1 and Pr2 activities were differentially inhibited by various protease inhibitors. Pr1, Pr2, esterase, aminopeptidase and N-acetylglucosaminidase (exochitinase) activities were present during penetration as detected directly following desorption from fungal and cuticle components. The proteases produced in situ were fractionated, and were shown by immunological and enzymological criteria to be the same as those produced in culture media. The sequence of enzyme appearance in situ showed that production of proteolytic enzymes precedes exochitinase production. No production of endochitinase was found before or during hyphal penetration of the cuticle.
Effect of Medium on H <sub>2</sub> O <sub>2</sub> Levels and Peroxidase-Like Activity by <i>Mycoplasma pneumoniae</i> (1971)

H(2)O(2) levels accumulated by Mycoplasma pneumoniae can be influenced by carbon source, different horse sera, yeast extract, thallium acetate, or growth in a simplified, dialyzed medium. Thus, increased levels of H(2)O(2) were detected by growth in glycerol, by omission of thallium acetate, and by the use of dialyzed medium. The ability of M. pneumoniae in the presence of glucose, but not with fructose, mannose, or glycerol, to remove both endogenous and exogenous H(2)O(2) suggests an inducible peroxidase similar to bacterial enzymes in streptococci. This peroxidase-like activity is in turn influenced by the complex interplay of medium components, explaining perhaps some of the variability of H(2)O(2) accumulations observed with glucose. A survival value has been suggested for this "peroxidase-like" activity.
Use of platinum electrodes for the electrochemical detection of bacteria (1978)

Platinum electrodes with surface area ratios of four to one were used to detect and enumerate a variety of gram-positive and gram-negative organisms. Linear relationships were established between inoculum size and detection time. End points for platinum electrodes were similar to those obtained with a platinum-reference electrode combination. Shape of the overall response curves and length of detection times for gram-positive organisms were markedly different than those for the majority of gram-negative species. Platinum electrodes are better than the platinum-reference electrode combination because of cost, ease of handling, and clearer definition of the end point.
The Fatty Acid Composition of Phosphatidylglycerol and Sulfoquinovosyldiacylglycerol of Higher Plants in Relation to Chilling Sensitivity (1986)

The fatty acid composition of phosphatidylglycerol and sulfoquinovosyldiacylglycerol has been measured in the leaves of 27 species of higher plants from six families whose members differed in their degrees of chilling sensitivity. The content of high melting point fatty acids (represented by the sum of hexadecanoic, trans-3-hexadecenoic and octadecanoic acids) in phosphatidylglycerols varied little between members of the same plant family and was not obviously related to the relative chilling sensitivity of members of that family. The saturated fatty acid content (hexadecanoic + octadecanoic acids) of sulfoquinovosyldiacylglycerols also appeared to be characteristic of a plant family, although some exceptions were found. In one case, (Carica papaya) the content of saturated fatty acids in sulfoquinovosyldiacylglycerol was sufficiently high to suggest that this lipid could undergo phase separations above 0 degrees C. It is concluded that the content of high melting point fatty acids in leaf phosphatidylglycerol is not a direct indication of the chilling sensitivity of a plant, but rather may be a reflection of the genetic origin of that plant.
Purification of the regulatory component of adenylate cyclase. (1980)

The regulatory component (G/F) of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] from rabbit liver plasma membranes has been purified essentially to homogeneity. The purification was accomplished by three chromatographic procedures in sodium cholate-containing solutions, followed by three steps in Lubrol-containing solutions. The specific activity of G/F was enriched 2000-fold from extracts of membranes to 3-4 mumol x min-1 x mg-1 (reconstituted adenylate cyclase activity). Purified G/F reconstitutes guanine nucleotide-, fluoride-, and hormone-stimulated adenylate cyclase activity in the adenylate cyclase-deficient variant of S49 murine lymphoma cells. G/F also recouples hormonal stimulation of the enzyme in the uncoupled variant of S49. Preparations of pure G/F contain three polypeptides with approximate molecular weights of 52,000, 45,000, and 35,000. The active G/F protein behaves as a multisubunit complex of these polypeptides. Treatment of G/F with [32P]NAD+ and cholera toxin covalently labels the molecular weight 52,000 and 45,000 polypeptides with 32P.
THE ADVANCED ENCRYPTION STANDARD (2002)

In this paper, we describe the Advanced Encryption Standard (AES), which has been approved after an international competition by the National Institute of Standards and Technology.
Delaying physical register allocation through virtual-physical registers (1999)

Register file access time represents one of the critical delays of current microprocessors, and it is expected to become more critical as future processors increase the instruction window size and the issue width. This paper presents a novel physical register management scheme that allows for a late allocation (at the end of execution) of registers. We show that it can provide significant savings in number of registers and thus, it can significantly shorten the register file access time. The approach is based on virtual-physical registers, which we presented in a previous work, extended with a new register allocation policy. This policy consists of an on-demand allocation in order to maximize the register usage, combined with a stealing mechanism that prevents older instruction from being delayed by younger ones. This shortens the average number of cycles that each physical register is allocated, and allows for an early execution of instructions since they can obtain a physical register for its destination earlier than with the conventional scheme. Early execution is especially beneficial for branches and memory operations, since the former can be resolved earlier and the latter can prefetch their data in advance.
Enhancing the brightness of thin-film electroluminescent displays by improving the emission process (1994)

AC thin film electroluminescent (ACTFEL) devices emit light laterally in addition to conventional surface emission. Because of the waveguiding in ACTFEL devices, lateral emission is stronger than surface emission. However, the former is ignored for displays. This note demonstrates that mirrors can be fabricated into substrates to reflect upward the lateral emission, which can then be summed with surface emission for displays. Use of standard silicon and thin film technology has demonstrated that the reflected lateral emission is a factor of three brighter than conventional surface emission. hence the effective efficiency of out-coupling the light has been improved for an ACTFEL device.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
NILM Techniques for Intelligent Home Energy Management and Ambient Assisted Living: A Review (2019)

The ongoing deployment of smart meters and different commercial devices has made electricity disaggregation feasible in buildings and households, based on a single measure of the current and, sometimes, of the voltage. Energy disaggregation is intended to separate the total power consumption into specific appliance loads, which can be achieved by applying Non-Intrusive Load Monitoring (NILM) techniques with a minimum invasion of privacy. NILM techniques are becoming more and more widespread in recent years, as a consequence of the interest companies and consumers have in efficient energy consumption and management. This work presents a detailed review of NILM methods, focusing particularly on recent proposals and their applications, particularly in the areas of Home Energy Management Systems (HEMS) and Ambient Assisted Living (AAL), where the ability to determine the on/off status of certain devices can provide key information for making further decisions. As well as complementing previous reviews on the NILM field and providing a discussion of the applications of NILM in HEMS and AAL, this paper provides guidelines for future research in these topics.
Molecular cloning of rat interleukin 4 cDNA and analysis of the cytokine repertoire of subsets of CD4<sup>+</sup> T cells (1991)

Rat peripheral CD4+ T cells may be subdivided into two functionally distinct subpopulations (OX-22highCD4+ and OX-22lowCD4+) on the basis of their reactivity with the monoclonal antibody MRCOX-22, which recognizes a restricted epitope on the leukocyte common antigen (LCA, CD45). Previous studies have demonstrated the increased activity of the OX-22highCD4+ subset in assays of cell-mediated reactivity, whereas the reciprocal OX-22lowCD4+ subset provides the majority of help for B cells in secondary antibody responses. Analyses of in vivo function have subsequently shown that the autoreactive activity associated with the OX-22highCD4+ subset can be inhibited through a suppressor activity within the OX-22lowCD4+ subset, indicating a further immunoregulatory role for these cells. Since the differential production of lymphokines such as interferon-gamma (IFN-gamma) and interleukin 4 (IL4) is believed to regulate alternative effector responses to a particular antigen, we have compared the lymphokine mRNA profiles of activated OX-22highCD4+ and OX-22lowCD4+ subsets using nucleic acid probes specific for rat IL 2, IFN-gamma and IL4, the latter of which has been isolated by a polymerase chain reaction cloning technique and its sequence is described. A higher frequency of cells expressed IL 2 mRNA in the OX-22high subset, in accordance with the relative levels of IL2 protein produced. In contrast, more IFN-gamma mRNA was detected in the OX-22lowCD4+ subset 24 h after mitogenic stimulation although these cells have consistently been shown to produce less IFN-gamma protein than the OX-22highCD4+ subset. This apparent paradox was resolved by the finding that the IFN-gamma mRNA levels in the OX-22lowCD4+ subset declined rapidly after 24 h while the levels continued to rise in the OX-22highCD4+ population such that at 48 h the relative levels were reversed. We have also demonstrated a higher level of IL4 mRNA expression within the OX-22lowCD4+ subset, which is undoubtedly involved in the increased B cell helper activity mediated by this subpopulation and may be responsible, in part, for their active suppression of cell-mediated immune responses.
The Amino-Terminal Peptide of HIV-1 gp41 Interacts with Human Serum Albumin (1993)

Structural and functional studies were made to assess interactions between human serum albumin (HSA) and the amino-terminal peptide (FP-I; 23-residue peptide 519-541) of glycoprotein 41,000 (gp41) of human immunodeficiency virus type-1 (HIV-1). Circular dichroism (CD) spectroscopy indicated that the peptide binds to albumin with dominant alpha-helical character. Peptide binding to albumin was also examined using FP-I spin labeled at either the amino-terminal alanine (FP-II; residue 519) or methionine (FP-III; position 537). Electron spin resonance (ESR) spectra of FP-II bound to HSA at 38 degrees C indicated that the spin label at the amino-terminal residue (Ala-519) was motionally restricted. The ESR spectrum of 12-nitroxide stearate (12-NS)-labeled HSA was identical to that obtained with FP-II, indicating that the reporter groups for the 12-NS and FP-II probes are similarly bound to albumin. Contrarily, ESR spectra of HSA labeled with FP-III indicated high mobility for the reporter group (Met-537) at the aqueous-protein interface. This suggests that the N-terminal gp41 peptide binds as an alpha helix (residues 519-536) to fatty acid sites on HSA, such that Ala-519 of the peptide residues in the interior of the protein while Met-537 lies outside the protein in aqueous solution. It is also of interest that addition of HSA to human red blood cells dramatically reduced the ability of FP-I to induce hemolysis, presumably through peptide-albumin binding that inhibited FP-I interactions with red cell membranes. The significance of these results focuses on the following three points. The first is that high serum levels of albumin may limit the efficacy of anti-HIV therapies using peptides based on the N-terminal gp41 domain. The second is that the elucidation of FP-I and HSA interactions with physical techniques may provide clues on the molecular features underlying viral FP-I combination with receptors on the target cell surface. Last, the affinity of albumin for the N-terminal gp41 peptide may play a subordinate role in the blocking of HIV infectivity in vitro that has been reported for chemically modified albumins.
Fully aliphatic polyimides from adamantane‐based diamines for enhanced thermal stability, solubility, transparency, and low dielectric constant (2006)

Abstract We have synthesized a series of fully aliphatic polyimides (APIs) from bicyclo[2,2,2]oct‐7‐ene‐2,3,5,6‐tetracarboxylic dianhydride (BOCA) and various aliphatic diamines, including linear aliphatic, flexible alicyclic, and rigid adamantyl diamines. We performed the polymerization reactions using one‐step syntheses in m ‐cresol at elevated temperatures without the isolation of poly(amic) acid. The chemical composition and structure of the polymers were characterized by nuclear magnetic resonance (NMR) and infrared (IR) spectrometry. The characterization data are reported from analyses using gel permeation chromatography (GPC), thermogravimetric analysis (TGA), differential scanning calorimeter (DSC), and wide‐angle X‐ray diffraction (WXAD) measurements. The polyimides are also subjected to solubility, solution viscosity, tensile strength, transparency, and dielectric constant measurements. The resultant polyimides possess well‐controlled molecular weight, reasonable intrinsic viscosity, good transparency, enhanced solubility, low dielectric constants, and high glass transition temperature, together with marginal thermal and mechanical stability. These properties were enhanced in copolyimides containing equimolar amounts of rigid and flexible moieties. These rigid‐rod APIs derived from the alicyclic dianhydride and aliphatic diamines are promising candidates as advanced materials for future applications in micro‐ and photoelectronic devices. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 3316–3326, 2006
Copper Nanoparticles on Charcoal for Multicomponent Catalytic Synthesis of 1,2,3‐Triazole Derivatives from Benzyl Halides or Alkyl Halides, Terminal Alkynes and Sodium Azide in Water as a “Green” Solvent (2009)

Abstract A one‐pot procedure for synthesis of 1,2,3‐triazole derivatives via the three‐component coupling (TCC) reaction between terminal alkynes, benzyl or alkyl halides, and sodium azide in the presences of 1 mol% nanoparticles copper/carbon (Cu/C) catalyst has been developed. The catalyst showed high catalytic activity and 1,4‐regioselectivity for the [3+2] Huisgen cycloaddition in water as a “green” solvent and good to excellent yields were obtained in all cases. This procedure eliminates the need to handle organic azides, and they are generated in situ . The reaction has a broad scope and is especially practical for the synthesis of new azacrown ether and anthraquinone derivatives of triazole. The heterogeneous catalysts were fully characterized by scanning electron microscopy (SEM), atomic forced microscopy (AFM), X‐ray diffraction (XRD), inductively coupled plasma (ICP) analysis and FT‐IR experimental techniques. The catalyst was recycled ten times without significant loss of activity.
TAK-701, a Humanized Monoclonal Antibody to Hepatocyte Growth Factor, Reverses Gefitinib Resistance Induced by Tumor-Derived HGF in Non–Small Cell Lung Cancer with an <i>EGFR</i> Mutation (2010)

Most non-small cell lung cancer (NSCLC) tumors with an activating mutation of the epidermal growth factor receptor (EGFR) are initially responsive to EGFR tyrosine kinase inhibitors (TKI) such as gefitinib but ultimately develop resistance to these drugs. Hepatocyte growth factor (HGF) induces EGFR-TKI resistance in NSCLC cells with such a mutation. We investigated strategies to overcome gefitinib resistance induced by HGF. Human NSCLC cells with an activating EGFR mutation (HCC827 cells) were engineered to stably express HGF (HCC827-HGF cells). HCC827-HGF cells secreted large amounts of HGF and exhibited resistance to gefitinib in vitro to an extent similar to that of HCC827 GR cells, in which the gene for the HGF receptor MET is amplified. A MET-TKI reversed gefitinib resistance in HCC827-HGF cells as well as in HCC827 GR cells, suggesting that MET activation induces gefitinib resistance in both cell lines. TAK-701, a humanized monoclonal antibody to HGF, in combination with gefitinib inhibited the phosphorylation of MET, EGFR, extracellular signal-regulated kinase, and AKT in HCC827-HGF cells, resulting in suppression of cell growth and indicating that autocrine HGF-MET signaling contributes to gefitinib resistance in these cells. Combination therapy with TAK-701 and gefitinib also markedly inhibited the growth of HCC827-HGF tumors in vivo. The addition of TAK-701 to gefitinib is a promising strategy to overcome EGFR-TKI resistance induced by HGF in NSCLC with an activating EGFR mutation.
Drilling Fluids (2004)

Abstract Drilling fluids (muds) play a vital role in the drilling of oil and gas wells. These muds are typically complex chemical mixtures formulated to perform a variety of functions under a wide range of down hole temperatures and pressures, but yet are nondamaging to the environment and safe for personnel to handle. These fluids are circulated down the hollow drill string and up the annulus, formed by the drill string and the hole to remove formation material dislodged by the bit. The fluids must have sufficient density to offset formation pressures to prevent a blowout and to maintain a stable well bore, but be formulated to prevent excessive loss of fluid to permeable formations. Other important functions include lubrication of the drill bit and drill string, collection of information about subsurface formations, and noncorrosivity to surface and down hole equipment. Drilling fluids are composed of liquids (water, petroleum oils, and other organic liquids, eg, synthetic brine fluids), dissolved inorganic and organic additives, and suspended, finely divided solids of various types. This article describes drilling fluid classification, properties, materials used as drilling fluid additives, testing, economics, and an update on current environmental restrictions on drilling fluids/muds in the North Sea and in the Gulf of Mexico. A detailed section has been devoted to clear brine drilling fluids (CBFs) that are used in completion and workover operations. Also presented is a selection of new and recent drilling fluid formulations from the patent literature.
A framework for constructing semantically composable feature models from natural language requirements (2009)

Software Product Line Engineering (SPLE) requires the construction of feature models from large, unstructured and heterogeneous documents, and the reliable derivation of product variants from the resulting model. This can be an arduous task when performed manually, and can be error-prone in the presence of a change in requirements. In this paper we introduce a tool suite which automatically processes natural-language requirements documents into a candidate feature model, which can be refined by the requirements engineer. The framework also guides the process of identifying variant concerns and their composition with other features. We also provide language support for specifying semantic variant feature compositions which are resilient to change. We show that feature models produced by this framework compare favourably with those produced by domain experts by application to a real-life industrial example.
Cerebral blood flow in normal pressure hydrocephalus. (1987)

A xenon-133 method was used to measure cerebral blood flow (CBF) before and after cerebrospinal fluid (CSF) removal in patients with normal pressure hydrocephalus (NPH). Preliminary results suggested that shunting should be performed on patients whose CBF increased after CSF removal. There was a significant increase in CBF in patients with NPH, which was confirmed by the favorable outcome of 88% of patients shunted. The majority of patients with senile and presenile dementia showed a decrease or no change in CBF after CSF removal. It is suggested that although changes in CBF and clinical symptoms of NPH may have the same cause, i.e., changes in the cerebral intraparenchymal pressure, there is no simple direct relation between these two events. The mechanism underlying the loss of autoregulation observed in NPH is also discussed.