gebhart/patrepeval huggingface dataset:
Sphingosine kinase 1 as a potential therapeutic target in epithelial ovarian cancer (2014)

Sphingosine kinase 1 (SK1) is over-expressed in multiple types of human cancer. SK1 has growth-promoting effects and has been proposed as a potential therapeutic target. We investigated the therapeutic effects of SK1 inhibition in epithelial ovarian carcinoma (EOC). SK1 siRNA or inhibitors were tested in EOC cell lines, including A2780, SKOV3ip1, A2780-CP20, SKOV3-TR, ES2 and RMG2. Cells were treated with SK inhibitor or FTY720, and cell proliferation, apoptosis, angiogenesis and invasion were examined by MTT, FACS, ELISA and wound-healing assays, respectively. In vivo experiments were performed to test the effects of FTY720 on tumor growth in orthotopic mouse xenografts of EOC cell lines A2780 or SKOV3ip1 and a patient-derived xenograft (PDX) model of clear cell ovarian carcinoma (CCC). Blocking SK1 with siRNA or inhibitors significantly reduced proliferation, angiogenesis and invasion, and increased apoptosis in chemosensitive (A2780 and SKOV3ip1) and chemoresistant (A2780-CP20, SKOV3-TR, ES2 and RMG2) EOC cells. SK1 inhibitors also decreased the intracellular enzymatic activity of SK1. Furthermore, FTY720 treatment significantly decreased the in vivo tumor weight in xenograft models of established cell lines (A2780 and SKOV3ip1) and a PDX model for CCC compared to control (p < 0.05). These results support therapeutic targeting of SK1 as a potential new strategy for EOC.
Synthesis and characterization of cross-conjugated oligo(phenylene enynylene)s (2005)

The synthesis and characterization of a series of cross-conjugated oligo(phenylene enynylene)s via the Sonogashira protocol is reported. The structural properties of these oligomers have been established by 1 H and 13 C NMR and IR spectroscopies, as well as mass spectrometry. Their electronic absorption and emission behavior has been investigated via UV/Vis and fluorescence spectroscopy. The results of this study demonstrate that electronic communication along the conjugated framework of these oligomers is limited due to the presence of a cross-conjugated enyne framework and arylene fragments.
Improved preparation of 1,3-cyclopentanedione (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImproved preparation of 1,3-cyclopentanedioneJohn M. McIntosh and Pierre BeaumierCite this: J. Org. Chem. 1972, 37, 18, 2905–2906Publication Date (Print):September 1, 1972Publication History Published online1 May 2002Published inissue 1 September 1972https://pubs.acs.org/doi/10.1021/jo00983a027https://doi.org/10.1021/jo00983a027research-articleACS PublicationsRequest reuse permissionsArticle Views516Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
ChemInform Abstract: PRIMARY PRODUCTS IN PHOTOCYCLOADDITIONS OF 9‐ANTHRACENECARBONITRILE TO α‐TERPINENE AND 1‐METHOXY‐1,3‐CYCLOHEXADIENE (1981)

Abstract Unter Lichteinwirkung addiert sich α‐Terpinen (II) an das Carbonitril (I), wobei neben dem Dimer von (I) die Addukte (III) und (IV) entstehen.
ION-CONDUCTING POLYMERS* (2002)

*Reprinted from Functional Monomers and Polymers, 2nd ed.; Takemoto, K., Ottenbrite, R.M., Kamachi, M., Eds.; Marcel Dekker, Inc.: New York; 1997, 387–431. Acknowledgments Notes *Reprinted from Functional Monomers and Polymers, 2nd ed.; Takemoto, K., Ottenbrite, R.M., Kamachi, M., Eds.; Marcel Dekker, Inc.: New York; 1997, 387–431.
ChemInform Abstract: Recent Advances in the Chemistry of Stable Simple Enols (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
The molecular basis of ATM-dependent dimerization of the Mdc1 DNA damage checkpoint mediator (2012)

Mdc1 is a large modular phosphoprotein scaffold that maintains signaling and repair complexes at double-stranded DNA break sites. Mdc1 is anchored to damaged chromatin through interaction of its C-terminal BRCT-repeat domain with the tail of γH2AX following DNA damage, but the role of the N-terminal forkhead-associated (FHA) domain remains unclear. We show that a major binding target of the Mdc1 FHA domain is a previously unidentified DNA damage and ATM-dependent phosphorylation site near the N-terminus of Mdc1 itself. Binding to this motif stabilizes a weak self-association of the FHA domain to form a tight dimer. X-ray structures of free and complexed Mdc1 FHA domain reveal a 'head-to-tail' dimerization mechanism that is closely related to that seen in pre-activated forms of the Chk2 DNA damage kinase, and which both positively and negatively influences Mdc1 FHA domain-mediated interactions in human cells prior to and following DNA damage.
Intelligent Resource Scheduling at Scale: A Machine Learning Perspective (2018)

Resource scheduling in a computing system addresses the problem of packing tasks with multi-dimensional resource requirements and non-functional constraints. The exhibited heterogeneity of workload and server characteristics in Cloud-scale or Internet-scale systems is adding further complexity and new challenges to the problem. Compared with,,,, existing solutions based on ad-hoc heuristics, Machine Learning (ML) has the potential to improve further the efficiency of resource management in large-scale systems. In this paper we,,,, will describe and discuss how ML could be used to understand automatically both workloads and environments, and to help to cope with scheduling-related challenges such as consolidating co-located workloads, handling resource requests, guaranteeing application's QoSs, and mitigating tailed stragglers. We will introduce a generalized ML-based solution to large-scale resource scheduling and demonstrate its effectiveness through a case study that deals with performance-centric node classification and straggler mitigation. We believe that an MLbased method will help to achieve architectural optimization and efficiency improvement.
Deep tissue imaging using spectroscopic analysis of multiply scattered light (2014)

Scattering limits the penetration depth of most optical imaging techniques. Efforts to overcome this limitation often require complex optical or computational schemes. We have developed a new method of assessing tissue properties based on spectroscopic analysis of multiply scattered light. The technique, multispectral multiple-scattering low-coherence interferometry (ms2/LCI), uses coherence and spatial gating to produce images of tissue optical properties up to 9 mm deep, with millimeter-scale resolution. The capabilities of ms2/LCI are demonstrated using tissue phantoms composed of chicken breast. Discrimination of diseased and healthy tissues is shown through imaging and analysis of burns in ex vivo human skin samples. Our technique may provide a powerful way to assess burn depth and progression in sensitive, burned tissues where physical contact is undesirable.
Studying on extraction method for semantic relationship among topic words (2015)

A concept lattice based method to extract the semantic relationship among topic words proposed. By counting the topic words frequency in the text, topic words are classified as low-frequency, middle-frequency, high-frequency topic words. It's the base to forms the semantic space of topic words of semantic network. Using concept lattice, semantic relationships in the topic level are extracted. The relationships include similar relationship and imply relationship. A good result has been obtained in the vertical search engine object for clothing area. The extract method can also be directly applied to related search and query expansion of information retrieval etc.
Real-Time Detection of the Mutation Responsible for Progressive Rod-Cone Degeneration in Labrador Retriever Dogs Using Locked Nucleic Acid TaqMan Probes (2009)

Progressive rod-cone degeneration (prcd) is a late onset, autosomal recessive, inherited disease in dogs caused by a G > A substitution in the PRCD locus. prcd has been reported in more than 18 breeds, including Labrador Retriever dogs. In this study, a real-time polymerase chain reaction (PCR) assay, exploiting the features of locked nucleic acid (LNA) fluorescent-labeled probes, was developed to genotype the sequence variants responsible for the disease. Two Labrador Retrievers were diagnosed with prcd by ophthalmological examination performed by a panelist of the Italian hereditary eye disease control program. The 2 dogs, as well as 8 related and 14 unrelated Labrador Retrievers, were genotyped with both direct sequencing of the disease locus and real-time LNA TaqMan PCR assay. Even though the region surrounding the mutation was predicted to be highly structured, making probe annealing difficult, the real-time PCR assay allowed researchers to correctly genotype the dogs in all cases with a sensitivity threshold of 4 ng/reaction of genomic DNA. A real-time PCR assay will allow a high-throughput analysis of a larger cohort of dogs, thereby enabling researchers to investigate the prevalence of the mutated allele in the affected breeds.
Drucklose Direktfluorierung: Eine einfache Methode zur präparativen Synthese von neuen Fluorierungsreagenzien (1990)

Large-scale synthesis of the new saccharin-derived N-fluorosultam 1 is described, starting from readily available saccharin 2. A newly designed fluorination apparatus, which allows the preparation of 100-g quantities of 1 at atmospheric pressure, is discussed in detail.
Influence of cycling position and crosswinds on performance and aerodynamics (2015)

Wind is fundamentally important to cyclists since it affects their performance, bicycle control, balance and road safety. The overall aim of this research is to improve the understanding of the effect of cycling position and crosswinds on the aerodynamics and performance of a cyclist.\nThe first part of this thesis focuses on cycling position. To minimise air resistance, cyclists lower their torso to become more aerodynamic. Results show that lowering torso angle is associated with impairment of physiological functioning, which can be explained by a combination of mechanical and muscular factors. Consequently, there is a trade-off between aerodynamic gains and physiological impairment. Therefore a mathematical model has been developed predicting the optimal torso angle at different cycling speeds.\nThe second part of this thesis aims to understand the flow mechanics around a cyclist subjected to crosswinds. Both wind tunnel experiments and computational fluid dynamics simulations of the flow around a cyclist are used to reveal the effect of crosswinds on cyclists in different cycling positions.\nThis thesis provides a unique approach to study cycling performance and safety by jointly investigating the human physiology and aerodynamic performance.
The immunological synapse: a cause or consequence of T-cell receptor triggering? (2011)

The immunological synapse forms as a result of the tight apposition of a T cell with an antigen-presenting cell (APC) and it is the site where the T-cell receptor (TCR) is triggered by its antigen ligand, the peptide-MHC complex present in the APC membrane. The immunological synapse was initially characterized in the T-cell membrane as three concentric rings of membrane receptors and their underlying cytoskeletal and signalling proteins. The inner circle, or central supramolecular activation cluster (cSMAC), concentrates most of the TCR and CD28, and it is surrounded by the peripheral SMAC that is formed by integrins. Finally, the most external ring or distal SMAC (dSMAC) is where proteins with large ectodomains are located, such as CD43 and CD45, far from the cSMAC. This arrangement was initially thought to be responsible for maintaining sustained TCR signalling, however, this typical concentric bull's-eye pattern is not found in the immunological synapses formed with the APCs of dendritic cells. Interestingly, TCR signalling has been detected in microclusters formed in the dSMAC area and it extinguishes as the TCRs reach the cSMAC. Hence, it appears that TCR signalling and full T-cell activation do not require the formation of the cSMAC and that this structure may rather play a role in TCR down-regulation, as well as participating in the polarized secretion of lytic granules. Here, we shall review the historical evolution of the role of the cSMAC in T-cell activation, finally discussing our most recent data indicating that the cSMAC serves to internalize exhausted TCRs by phagocytosis.
Pathogenesis and treatment of mitochondrial myopathies: recent advances. (2010)

In this brief review, I have highlighted recent advances in several areas of mitochondrial medicine, including mtDNA-related diseases, mendelian mitochondrial encephalomyopathies, and therapy. The pathogenic mechanisms of mtDNA mutations, especially those affecting mitochondrial protein synthesis, are still largely unknown. The pathogenicity of homoplasmic mtDNA mutations has become evident but has also called attention to modifying nuclear genes, yet another example of impaired intergenomic signaling. The functional significance of the homoplasmic changes associated with mitochondrial haplogroups has been confirmed. Among the mendelian disorders, a new form of "indirect hit" has been described, in which the ultimate pathogenesis is toxic damage to the respiratory chain. Three therapeutic strategies look promising: (i) allogeneic hematopoietic stem cell transplantation in MNGIE (mitochondrial neurogastrointestinal encephalomyopathy); (ii) bezafibrate, an activator of PGC-1alpha, has proven effective in animal models of mitochondrial myopathy; and (iii) pronucleus transfer into a normal oocyte is effective in eliminating maternal transmission of mtDNA, thus preventing the appearance of mtDNA-related disorders.
[Difluro(phosphono)methyl]phenylalanine-containing peptide inhibitors of protein tyrosine phosphatases (1999)

Peptides containing the non-hydrolysable phosphotyrosine analogue 4-[difluro(phosphono)methyl]phenylalanine [Phe(CF2P)] were synthesized and tested as inhibitors of the protein tyrosine phosphatases (PTPs) PTP1B, CD45, PTPbeta, LAR and SHP-1. We have identified peptides containing two adjacent Phe(CF2P) residues as potent inhibitors of PTPs. The tripeptide having the sequence Glu-Phe(CF2P)-Phe(CF2P) is a potent and selective inhibitor of PTP1B. This peptide inhibits PTP1B with an IC50 of 40 nM, which is at least 100-fold lower than with other PTPs. A second tripeptide, Pro-Phe(CF2P)-Phe(CF2P), is most potent against PTPbeta, with an IC50 of 200 nM, and inhibits PTP1B with an IC50 of 300 nM. These data suggest that it is possible to develop selective, active-site-directed, reversible, potent inhibitors of PTPs.
A novel reconfigurable power amplifier structure for multi-band and multi-mode portable wireless applications using a reconfigurable die and a switchable output matching network (2009)

A novel reconfigurable power amplifier structure for multi-band and multi-mode applications is presented. Included in the design are a reconfigurable output matching network and a reconfigurable die. To demonstrate the feasibility of the concept, a 900 MHz/1.6 GHz reconfigurable power amplifier with 33.8 dBm/29.4 dBm output power has been designed as a multi-chip module to validate the multi-band and multi-mode application. The new topology makes possible a 50% size reduction of the GaAs die and at least a 20% size reduction of the modules for two or more services. The topology is flexible and can be easily expanded to cover additional standards.
Short-Term Fluctuations in Petroleum Consumption (1953)

In this article Mr Butler not only shows how he has evolved a method of estimating the trend in petroleum consumption but also expresses the uncertainty in the resulting forecasts by giving limits within which short‐term fluctuations may be expected to lie.
Screening of Feed Components for Salmonella with Polyvalent H. Agglutination (1968)

The application of polyvalent H serology for screening certain feed components for Salmonella was evaluated. In a comparative study of 1,894 suspicious or known positive samples, Salmonella organisms were detected in 1,141 samples with the conventional method and in 1,134 samples with the polyvalent H method. A statistical analysis of the results obtained by both methods indicated that the polyvalent H method is as reliable as the conventional method. Salmonellae can be detected by this method within 60 hr, whereas conventional methods require at least 4 days. The speed and reliability of the polyvalent H method are desirable for routine quality assurance.
Structure and Association of Human Lactoferrin Peptides with<i>Escherichia coli</i>Lipopolysaccharide (2004)

An 11-amino-acid amphipathic synthetic peptide homologous to a helical region on helix 1 of human lactoferrin HLP-2 exhibited bactericidal activity against Escherichia coli serotype O111, whereas an analogue synthesized with Pro substituted for Met, HLP-6, had greatly reduced antimicrobial activity. The bactericidal activity of HLP-2 was 10-fold greater than that of HLP-6 in both buffer and growth medium by time-kill assays. These assays also showed a pronounced lag phase that was both concentration and time dependent and that was far greater for HLP-2 than for HLP-6. Both peptides, however, were shown to be equally efficient in destabilizing the outer membrane when the hydrophobic probe 1-N-phenylnaphthylamine was used and to have the same lipopolysaccharide (LPS) binding affinity, as shown by polymyxin B displacement. Circular dichroism (CD) spectroscopy was used to study the structure and the organization of the peptides in solution and upon interaction with E. coli LPS. In the presence of LPS, HLP-2 and HLP-6 were found to bind and adopt a beta-strand conformation rather than an alpha-helix, as shown by nonimmobilized ligand interaction assay-CD spectroscopy. Furthermore, this assay was used to show that there is a time-dependent association of peptide that results in an ordered formation of peptide aggregates. The rate of interpeptide association was far greater in HLP-2 LPS than in HLP-6 LPS, which was consistent with the lag phase observed on the killing curves. These results allow us to propose a mechanism by which HLP-2 folds and self-assembles at the outer membrane surface before exerting its activity.
Manufacture of SiC Composite Coatings by HVOF (2001)

Abstract The main application of HVOF processes is the production of wear and/or corrosion protective coatings based on cermets (e.g. WC-Co). To reduce the weight of the sprayed volume, to increase the oxidation resistance and to reduce the costs of consumables SiC based cermets are of particular interest. The outstanding mechanical, thermal and chemical properties of SiC have made this material attractive for thermal spray applications. Thermal spraying of pure SiC is impossible, because of its decomposition at elevated temperatures. The development of suitable alloys as metallic binders enables the wetting of the SiC particles with reduced reactivity between the matrix and the hard particle during the thermal spray process. In the present work starting from the metallurgical concept of the metallic matrix to the production route from the atomization of the metallic matrices, the spray drying and sintering as well as the processability of the composites is discussed. The coatings are characterized concerning porosity and SiC content using optical and scanning electron microscopy, digital image analysis, X-ray diffraction and micro hardness testing. The wear resistance of the coatings is determined by oscillating wear test. Microstructure and performance of the SiC composite coatings are discussed with respect to technical applications.
The involvement of Bcl-2 family proteins in AKT-regulated cell survival in cisplatin resistant epithelial ovarian cancer (2016)

Many studies involving patients with cisplatin-resistant ovarian cancer have shown that AKT activation leads to inhibition of apoptosis. The aim of this study was to examine the potential involvement of the Bcl-2 family proteins in AKT-regulated cell survival in response to cisplatin treatment. Cisplatin-sensitive (PEO1) and cisplatin-resistant (PEO4) cells were taken from ascites of patients with ovarian cancer before cisplatin treatment and after development of chemoresistance. It was found that cisplatin treatment activated the AKT signaling pathway and promoted cell proliferation in cisplatin-resistant EOC cells. When AKT was transfected into nucleus of cisplatin-resistant ovarian cancer cells, DNA-PK was phosphorylated at S473. The activated AKT (pAKT-S473) in these cells inhibited the death signal induced by cisplatin thereby inhibiting cisplatin-mediated apoptosis. Results from this study showed that the combination of cisplatin, DNA-PK inhibitor NU7441, and AKT inhibitor TCN can overcome drug resistance, increase apoptosis, and re-sensitize PEO4 cells to cisplatin treatment. A decrease in apoptotic activity was seen in PEO4 cells when Bad was downregulated by siRNA, which indicated that Bad promotes apoptosis in PEO4 cells. Use of the Bcl-2 inhibitor ABT-737 showed that ABT-737 binds to Bcl-2 but not Mcl-1 and releases Bax/Bak which leads to cell apoptosis. The combination of ABT-737 and cisplatin leads to a significant increase in the death of PEO1 and PEO4 cells. All together, these results indicate that Bcl-2 family proteins are regulators of drug resistance. The combination of cisplatin and Bcl-2 family protein inhibitor could be a strategy for the treatment of cisplatin-resistant ovarian cancer.
Novel Processing of Electromagnetic Logging Measurements Through Metallic Casing (2014)

Abstract This paper presents a novel method to remove the casing effects from electromagnetic (EM) logging measurements through metallic casing. After the removal of the casing effects, the measurements are exactly the same as those measured in the borehole without the casing. As a result, the data can be processed (or inverted) in the same manner as openhole EM logging data, such as the array induction and tri-axial induction measurements. The method converts data with casing effects to casing-free data via simple but efficient correction algorithms based on the measurements of the impedance of the transmitters and receivers, from which the casing effects around the transmitters and receivers can be determined. In addition, the method can be used to determine the magnetic and electric properties of the casing. In addition to single well logging, the method could be particular useful for crosswell EM survey between two cased holes which the industry has not been able to solve. One such benefit is that it could make it possible to see resistivity differences due to ongoing processes (i.e. water flooding) in a producing field. The algorithms and the physics underlying them are discussed in details. Both non-magnetic casing and magnetic casing are considered in the paper.