gebhart/patrepeval huggingface dataset:
The primary structure of the antenna polypeptides of <i>Ectothiorhodospira halochloris</i> and <i>Ectothiorhodospira halophila</i> (1992)

Antenna polypeptides from two species of the family Ectothiorhodospiraceae have been investigated. By means of gel filtration and subsequent high-performance liquid chromatography, at least five polypeptides were isolated from each of Ectothiorhodospira halochloris and Ectothiorhodospira halophila. The majority of their primary structures was identified by Edman degradation. Comparison of these polypeptide sequences with the known primary structures of antenna polypeptides from various purple non-sulfur bacteria revealed interesting new aspects with regard to the structure of the core-peripheral antenna system. E. halochloris and E. halophila contain two pairs of alpha- and beta-polypeptides each with typical primary structure elements of core complexes, indicating a modified antenna complex organization.
Fundamental insight into the effect of carbodiimide crosslinking on cellular recognition of collagen-based scaffolds (2016)

Carbodiimide stabilised collagen is employed extensively for the fabrication of biologically active materials. Despite this common usage, the effect of carbodiimide crosslinking on cell-collagen interactions is unclear. Here we have found that carbodiimide crosslinking of collagen inhibits native-like, whilst increasing non-native like, cellular interactions. We propose a mechanistic model in which carbodiimide modifies the carboxylic acid groups on collagen that are essential for cell binding. As such we feel that this research provides a crucial, long awaited, insight into the bioactivity of carbodiimide crosslinked collagen. Through the ubiquitous use of collagen as a cellular substrate we feel that this is fundamental to a wide range of research activity with high impact across a broad range of disciplines.
Accent adaptation in speech user interface (2006)

This paper examines the impact of accent present in speech on the performance of speaker independent automatic speech recognition (ASR) systems. In this paper, we show that, the presence of accent in the speech can increase the error rate. We validate a fundamental assumption that a speaker independent ASR engine, trained by a variety of accents, performs poorer than an engine that is trained for a particular accent, when tested by the same accent. Based on the results, we propose a method to lower the recognition error rate and measure the improvement by first determining the accent of the utterance and then applying the appropriate ASR engine from a bank of engines trained for different accents. We show that applying this method, will results to an average decrease of 24% overall error rate. The results are encouraging for a future complementary work. The research was carried out on an HMM based speech recognizer and TIMIT database was used to train and test the ASR engine.
Thick unevenness compensation in a hot rolling mill having automatic gage control (2002)

Hot rolling products with improved quality features such as thickness profile, strip width, flatness and material mechanical properties, are in increasing demand. The project goal is to improve the production quality in an existing hot rolling mill at Aceralia Steel Company, Aviles, Spain. This paper describes the design and implementation of a supervisory system for real-time compensation of uneven thickness on both sides of a rolled strip. The design is based on a multivariable process model, whose parameters are calculated online using measurement data from the mill. As a result, a computer system was developed that corrects the automatic gage control output from online acquired mill operation data. Previously, this task was done manually and periodically by a human operator. Manual correction is tedious and error prone, since it is based on visual inspection. Moreover, the proposed method leads to notorious improvement in the output strip quality, as the correcting signal may be continuously applied before the uneven thickness effect is visible.
Reactions of Perchloryl Fluoride with Organic Compounds. I. Perchlorylation of Aromatic Compounds<sup>1</sup> (1958)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of Perchloryl Fluoride with Organic Compounds. I. Perchlorylation of Aromatic Compounds1C. E. Inman, R. E. Oesterling, and E. A. TyczkowskiCite this: J. Am. Chem. Soc. 1958, 80, 19, 5286–5288Publication Date (Print):October 1, 1958Publication History Published online1 May 2002Published inissue 1 October 1958https://pubs.acs.org/doi/10.1021/ja01552a069https://doi.org/10.1021/ja01552a069research-articleACS PublicationsRequest reuse permissionsArticle Views182Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Landscape of transcription in human cells (2012)

Eukaryotic cells make many types of primary and processed RNAs that are found either in specific subcellular compartments or throughout the cells. A complete catalogue of these RNAs is not yet available and their characteristic subcellular localizations are also poorly understood. Because RNA represents the direct output of the genetic information encoded by genomes and a significant proportion of a cell's regulatory capabilities are focused on its synthesis, processing, transport, modification and translation, the generation of such a catalogue is crucial for understanding genome function. Here we report evidence that three-quarters of the human genome is capable of being transcribed, as well as observations about the range and levels of expression, localization, processing fates, regulatory regions and modifications of almost all currently annotated and thousands of previously unannotated RNAs. These observations, taken together, prompt a redefinition of the concept of a gene.
Analysis and Controller Design Based on Linear Model for Heavy-Duty Vehicles (1998)

Abstract In this paper, we present the time domain, frequency domain and pole/zero analyses of a linearized model of heavy-duty vehicles (tractor-semitrailer type) for lateral control. These analyses are conducted to examine the steering response subject to variations of speed, road adhesion coefficient and look-ahead distance for the lateral position sensor. Based on these analyses, a linear controller for lane following is designed. The close-loop simulation is conducted to show the effectiveness of the linear controller.
Simulating a Multi-domain RFID System for Replacement Part Tracking (2009)

The efficient and reliable handling of replacement parts is of high importance for airlines. It can be supported by attaching an RFID tag to replacement parts that stores data identifying unambiguously the replacement part and further information about its installation and maintenance. Security is essential so that only authenticated and authorized entities can read and modify data according to the defined access permissions. Although the information is managed in a decentralized way, it has anyhow to be synchronized with the airlines backend database finally.This paper describes data format, certificate management and access control when using these passive RFID tags in a multi-domain scenario for replacement part tracking within the avionics industry. The demonstrator architecture design is described for simulating replacement part tracking. It allows simulating use cases of the multi-domain RFID system for replacement part tracking. Both simulated and real RFID tags can be used in the simulation.
Do Deficiencies of Endothelial Derived Relaxing Factor Contribute to Myocardial Stunning? (1993)

Myocardial stunning is a phenomenon in which ventricular function is depressed by prior ischemia and remains suboptimal when blood flow is resumed. It is also characterized by coronary vascular endothelial dysfunction that is related to the duration of ischemia and can result in protein leakage, myocardial hemorrhage, or increased coronary vascular resistance. It is thought that local vascular tone is regulated by endothelium derived relaxing factor (EDRF), a compound commonly believed to be identical to nitric oxide (NO). EDRF increases the effects of other vasodilators through the formation of cyclic guanosine monophosphate (cGMP) by guanylate cyclase. Patients with various cardiovascular disorders have been found to have dysfunction in the EDRF system, and EDRF paradoxically produces vasoconstriction in atherosclerotic arteries. Similarly, EDRF stimulation of vasodilation due to acetylcholine, calcium ionophores, or platelets appears to be reduced in coronary arteries that have been damaged by ischemia and reperfusion. By increasing EDRF production or inhibiting its breakdown, EDRF precursors such as L-arginine, the superoxide radical scavenger superoxide dismutase, nitroglycerin, and nitroprusside all cause vasodilation by increasing NO levels in the setting of myocardial ischemia. These therapies may also improve local "microvascular" function, thereby improving ventricular function.
Rhenium complexes of bidentate, bis-bidentate and tridentate N-heterocyclic carbene ligands (2015)

A series of eight Rhenium(I)-N-heterocyclic carbene (NHC) complexes of the general form [ReCl(CO)3(C^C)] (where C^C is a bis(NHC) bidentate ligand), [ReCl(CO)3(C^C)]2 (where C^C is a bis-bidentate tetra-NHC ligand) and [Re(CO)3(C^N^C)](+)[X](-) (where C^N^C is a bis(NHC)-amine ligand and the counter ion X is either the ReO4(-) or PF6(-)) have been synthesised using a Ag2O transmetallation protocol. The novel precursor imidazolium salts and Re(I) complexes were characterized by elemental analysis, (1)H and (13)C NMR spectroscopy and the molecular structures for two imidazolium salt and six Re(I) complexes were determined by single crystal X-ray diffraction. These NHC ligand systems are of interest for possible applications in the development of Tc-99m or Re-186/188 radiopharmaceuticals and as such the stability of two complexes of the form [ReCl(CO)3(C^C)] and [Re(CO)3(C^N^C)][ReO4] were evaluated in ligand challenge experiments using the metal binding amino acids L-histidine or L-cysteine. These studies showed that the former was unstable, with the chloride ligand being replaced by either cysteine or histidine, while no evidence for transchelation was observed for the latter suggesting that bis(NHC)-amine ligands of this type may be suitable for biological applications.
Selective Trafficking of KNOTTED1 Homeodomain Protein and Its mRNA Through Plasmodesmata (1995)

Plasmodesmata are intercellular organelles in plants that establish cytoplasmic continuity between neighboring cells. Microinjection studies showed that plasmodesmata facilitate the cell-to-cell transport of a plant-encoded transcription factor, KNOTTED1 (KN1). KN1 can also mediate the selective plasmodesmal trafficking of kn1 sense RNA. The emerging picture of plant development suggests that cell fate is determined at least in part by supracellular controls responding to cellular position as well as lineage. One of the mechanisms that enables the necessary intercellular communication appears to involve transfer of informational molecules (proteins and RNA) through plasmodesmata.
State-equations corresponding to a time-varying linear differential system (1967)

This letter presents an algorithm for constructing state-equations corresponding to a well-formed, linear, time-varying system L(t,p)y = R(t,p)u.
Four steps in managing migraine (1989)

The first step in management of migraine is convincing the patient of the diagnosis. Once this has been accomplished, the physician needs to identify precipitating factors. Common migraine triggers include stress, menstruation, use of oral contraceptives, and certain foods. The next step is choosing appropriate therapy. Analgesics are most commonly prescribed in North America; nonsteroidal antiinflammatory drugs, ergotamine, narcotics, corticosteroids, and other drugs are also useful. Prophylactic therapy should be considered when treatment of individual attacks fails, causes adverse effects, or leads to dependence.
Role of cyclooxygenase-2 in inflammation (1994)

ARACHIDONATE 12-LIPOXYGENASES: THEIR POSSIBLE PHYSIOLOGICAL AND PATHOLOGICAL ROLES S. Yamamoto~, N. Uedal, K. Yamamotol, 3. Tokunaga2, E. Shirakawa2, H. Shinkai2, M. Ogawa 3, T. Sato 3, I. Kudo 3, K. Inoue 3, H. Takizawa 3, T. Nagano 3, M. Hirobe3, N. Matsuki3, H. Saito 3 1) Department of Biochemistry, Tokushima University, School of Medicine, Tokushima 770, Japan 2) Japan Tobacco Inc., Central Pharmaceutical Research Institute, Takatsuki, Osaka 569, Japan 3) Faculty of Pharmaceudcal Sciences, the University of Tokyo, Tokyo 113, Japan
Significance and Mechanism of the Indirect Effect in Bacterial Cells. The Relative Protective Effect of Added Compounds in Escherichia coli B, Irradiated in Liquid and in Frozen Suspension (1969)

Tore Sanner, Alexander Pihl, Significance and Mechanism of the Indirect Effect in Bacterial Cells. The Relative Protective Effect of Added Compounds in Escherichia coli B, Irradiated in Liquid and in Frozen Suspension, Radiation Research, Vol. 37, No. 1 (Jan., 1969), pp. 216-227
Lens-specific expression of transforming growth factor beta1 in transgenic mice causes anterior subcapsular cataracts. (1998)

Transgenic mice were generated by microinjection of a construct containing a self-activating human TGF-beta1 cDNA driven by the lens-specific alphaA-crystallin promoter. Seven transgenic founder mice were generated, and four transgenic lines expressing TGF-beta1 were characterized. By postnatal day 21, mice from the four families exhibited anterior subcapsular cataracts. The lenses in these mice developed focal plaques of spindle-shaped cells that expressed alpha-smooth muscle actin, and that resembled the plaques seen in human anterior subcapsular cataracts. Transgenic mice showed additional ocular defects, including corneal opacification and structural changes in the iris and ciliary body. The corneal opacities were associated with increased exfoliation of the squamous layer of the corneal epithelium and increased DNA replication in the basal epithelium.
Primary structure of an archaebacterial transducer, a methyl-accepting protein associated with sensory rhodopsin I. (1992)

A methylated membrane protein of 97 kDa was suggested on the basis of mutant analysis to transduce signals from the phototaxis receptor sensory rhodopsin I to the flagellar motor in Halobacterium halobium. Here we report isolation of the proposed transducer protein, cloning of its gene based on partial protein sequences, the complete gene sequence, and analysis of the encoded primary structure. The 1611-base-pair gene termination codon overlaps the initiator ATG of the sopI gene, which encodes the sensory rhodopsin I apoprotein. The predicted size of 57 kDa for the methylated protein indicates an aberrant electrophoretic migration on SDS/polyacrylamide gels, as occurs with other acidic halophilic proteins. Putative promotor elements are located in an A+T-rich region upstream of the gene. Comparison of the translated nucleotide sequence with N-terminal sequence of the purified protein shows the protein is synthesized without a processed leader peptide and the N-terminal methionine is removed in the mature protein. The deduced protein sequence predicts two transmembrane helices near the N terminal that would anchor the protein to the membrane. Beyond this hydrophobic region of 46 residues, the remainder of the protein (536-amino acid residues total) is hydrophilic. The C-terminal 270 residues contain a region homologous to the signaling domains of eubacterial transducers (e.g., Escherichia coli Tsr protein), flanked by two regions homologous to the methylation domains of the transducer family. The protein differs from E. coli Tsr in that it does not have an extramembranous-receptor binding domain but instead has a more extended cytoplasmic region. Coexpression of the methyl-accepting protein gene (designated htrI) and sopI restores sensory rhodopsin I phototaxis to a mutant (Pho81) that contains a deletion in the htrI/sopI region. These results extend the eubacterial transducer family to the archaebacteria and substantiate the proposal that the methylated membrane protein functions as a signal-transducing relay between sensory rhodopsin I and cytoplasmic sensory-pathway components.
Preparation of an RGD ALB Conjugate (1992)

RGD (Arg-Gly-Asp) tripeptide was identified as the minimal active core peptide sequence common to adhesive proteins. In this paper, the authors report preparation of RGD containing peptide albumin conjugate (RGD ALB), and its effects on cellular adhesive function in vitro. RGD ALB was prepared via a coupling reaction of albumin with pentapep-tide (GRGDS; Gly-RGD-Ser) by water soluble carbodimide. Bovine endothelial cells (ECs) adhered to and spread well on a surface coated with RGD ALB, whereas few ECs adhered on surfaces coated with GRGESP-albumin conjugate (GRGESP peptide with little cell attachment activity; false control) and albumin. Cellular behavior, such as adhesion, spreading, growth, and migration, on surfaces coated with GRD-ALB, fibronectin (FN), and vitronectin (VN) were quantitatively examined. Adherent cell number on RGD ALB coated surfaces was larger than on those coated with FN and VN. Cell morphology on RGD ALB coated surfaces was similar to that on FN coated surfaces. The cell growth and migration activities on RGD ALB coated surfaces were almost equal to those coated with FN and VN. Thus, RGD ALB was found to promote cell adhesion, migration, and growth as effectively as fibronectin. This indicates that an artificial adhesive protein, simply derived with bioactive peptidyl li-gand, can find versatile applications in fields in which cellular events play a critical role—for example, extracellular matrices and wound healing promotion aids.
Vacuum Tube Voltmeter (1940)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVacuum Tube VoltmeterLloyd E. West and Rex J. RobinsonCite this: Ind. Eng. Chem. Anal. Ed. 1940, 12, 8, 476–479Publication Date (Print):August 1, 1940Publication History Published online1 May 2002Published inissue 1 August 1940https://pubs.acs.org/doi/10.1021/ac50148a019https://doi.org/10.1021/ac50148a019research-articleACS PublicationsRequest reuse permissionsArticle Views29Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Prp43: An RNA helicase-like factor involved in spliceosome disassembly (1997)

The Saccharomyces cerevisiae genes PRP2, PRP16, and PRP22 encode pre-mRNA splicing factors that belong to the highly conserved "DEAH" family of putative RNA helicases. We previously identified two additional members of this family, JA1 and JA2. To investigate its biological function, we cloned the JA1 gene and generated alleles carrying mutations identical to those found in highly conserved regions of other members of the DEAH family. A ja1 allele carrying a mutation identical to that in the temperature-sensitive (ts) prp22-1 gene conferred ts phenotype when integrated into the genome of a wild-type strain by gene replacement. Northern analysis of RNA obtained from the ts strain shifted to a nonpermissive temperature revealed accumulation of unspliced pre-mRNAs and excised intron lariats. Furthermore, analysis of splicing complexes showed that intron lariats accumulated in spliceosomes. The results presented indicate that JA1 encodes a pre-mRNA processing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA. We have therefore renamed this gene PRP43.
Sketch-based modeling with few strokes (2005)

We present a novel sketch-based system for the interactive modeling of a variety of free-form 3D objects using just a few strokes. Our technique is inspired by the traditional illustration strategy for depicting 3D forms where the basic geometric forms of the subjects are identified, sketched and progressively refined using few key strokes. We introduce two parametric surfaces, rotational and cross sectional blending, that are inspired by this illustration technique. We also describe orthogonal deformation and cross sectional oversketching as editing tools to complement our modeling techniques. Examples with models ranging from cartoon style to botanical illustration demonstrate the capabilities of our system.
Parallel database systems (1990)

The concept of parallel database machines consisting of exotic hardware has been replaced by a fairly conventional shared-nothing hardware base along with a highly parallel dataflow software architecture. Such a design provides speedup and scaleup in processing relational database queries. This paper reviews the techniques used by such systems, and surveys current commercial and research systems.
Somatic expansion behaviour of the (CTG)n repeat in myotonic dystrophy knock-in mice is differentially affected by Msh3 and Msh6 mismatch-repair proteins (2002)

The mechanism of expansion of the (CTG)n repeat in myotonic dystrophy (DM1) patients and the cause of its pathobiological effects are still largely unknown. Most likely, long repeats exert toxicity at the level of nuclear RNA transport or splicing. Here, we analyse cis- and trans-acting parameters that determine repeat behaviour in novel mouse models for DM1. Our mice carry 'humanized' myotonic dystrophy protein kinase (Dmpk) allele(s) with either a (CTG)84 or a (CTG)11 repeat, inserted at the correct position into the endogenous DM locus. Unlike in the human situation, the (CTG)84 repeat in the syntenic mouse environment was relatively stable during intergenerational segregation. However, somatic tissues showed substantial repeat expansions which were progressive upon aging and prominent in kidney, and in stomach and small intestine, where it was cell-type restricted. Other tissues examined showed only marginal size changes. The (CTG)11 allele was completely stable, as anticipated. Introducing the (CTG)84 allele into an Msh3-deficient background completely blocked the somatic repeat instability. In contrast, Msh6 deficiency resulted in a significant increase in the frequency of somatic expansions. Competition of Msh3 and Msh6 for binding to Msh2 in functional complexes with different DNA mismatch-recognition specificity may explain why the somatic (CTG)n expansion rate is differentially affected by ablation of Msh3 and Msh6.