gebhart/patrepeval huggingface dataset:
Computer algorithms to characterize individual subject EMG profiles during gait. (1992)

Three methods of precisely determining onset and cessation times of gait EMG were investigated. Subjects were 24 normal adults and 32 individuals with gait pathologies. Soleus muscle EMG during free speed level walking was obtained with fine wires, and was normalized by manual muscle test (%MMT). Linear envelopes were generated from the rectified, integrated EMG at each percent gait cycle (%GC) of each stride in individual gait trials. Three methods were used to generate EMG profiles for each tested subject. The ensemble average (EAV) was determined for each subject from the mean relative intensity of the linear envelopes. Low relative intensity or short duration EMG was removed from the ensemble average to create the intensity filtered average (IFA). The packet analysis method (PAC) created an EMG profile from the linear envelopes in successive strides whose respective centroid %GC locations were within +/- 15%GC of each other. Control values for onset and cessation times of individual gait trials were calculated after spurious outliers were removed. Mean onset and cessation times across subjects for control values and the experimental methods (EAV, IFA, and PAC) were calculated. Dunnett's test (p less than .05) was performed to compare control and experimental groups in patient and normal trials. EAV differed from control values for onsets (p less than .01), cessations (p less than .01), and durations (p less than .01) in both normal and patient trials. IFA and PAC had no significant differences from control value means. IFA was selected for clinical use as automatic analysis could be performed on all trials and a minimum number of decision rules were needed.
A High-Resolution Capacitance-to-Frequency Converter (1985)

This paper describes an integrated sampled-data sinusoidal oscillator with AGC amplitude stabilization, the frequency of which is proportional to an external low value capacitor (2-20 pF). The circuit, based on a switched-capacitor two-integrator loop, is insensitive to stray capacitances between sensing nodes and ground and features high resolution (16 bits) and excellent linearity (better than 0.1 percent) as well as very low power consumption (less than 0.1 mW). Such a "capacitance-to-frequency converter" is particularly suitable for interfacing variable-capacitance sensors (pressure transducers, accelerometers,... ) in applications requiring high accuracy but where signal bandwidth is limited to a few hundred hertz.
A G Protein γ Subunit Shares Homology with ras Proteins (1989)

Guanine nucleotide binding proteins (G proteins) that transduce signals from cell surface receptors to effector molecules are made up of three subunits, alpha, beta, and gamma. A complementary DNA clone that encodes a 71-amino acid protein was isolated from bovine brain; this protein contains peptide sequences that were derived from the purified gamma subunit of Gi and Go. The primary sequence of this G protein gamma subunit (G gamma) has 55 percent homology to the gamma subunit of transducin (T gamma) and also has homology to functional domains of mammalian ras proteins. The probe for isolating the clone was generated with the use of the polymerase chain reaction (PCR). The extent of divergence between T gamma and G gamma, the isolation of homologous PCR-generated fragments, and the differences between the predicted amino acid sequence of G gamma and that derived from the gamma subunit of Gi and Go indicate that gamma subunits are encoded by a family of genes.
A floor plan creation tool utilizing a smartphone with an ultrasonic sensor gadget (2016)

These days, the proposition that various applications in local indoor navigation have attracted significant attention recently is the provocative one. In order to improve and optimize the indoor navigation applications, we have to obtain the accurate floor plan of target place such as building and home environments. However, the belief that all the buildings provide us with accurate floor plan for indoor navigation is groundless. In this paper, we present a floor plan generator tool utilizing a smartphone integrated with an ultrasonic sensor gadget. In the measurement, the user completes a lap along the walls of all rooms. Then, the system estimates the accurate shape and size of the rooms. It leverages the inertial sensors, implemented in the smartphone, to track the user in the walking path. Moreover, the ultrasonic sensor in the gadget measures the distance between the path and walls. There are two main challenges to achieve optimized performance. The first challenge is that adjacent objects to, such as bookshelves, affect the accuracy in spatial layout estimation. To cope with this problem, we use a mixed Gaussian filter. The second challenge is that the narrow room, such as corridors, leads to the low accuracy. To alleviate this challenge, we implement two ultrasonic sensors in the reverse direction, and measure the distance between walls directly. The results from experimentations report improvement in shape and size estimation accuracy.
Haloacid Induced Alterations in Fertility and the Sperm Biomarker SP22 in the Rat Are Additive: Validation of an ELISA (2004)

Dibromoacetic acid (DBA) and bromochloroacetic acid (BCA) are prevalent disinfection by-products of drinking water that produce defects in spermatogenesis and fertility in adult rats. Previously we demonstrated that BCA compromises the fertility of cauda epididymal rat sperm and SP22, a sperm membrane protein that is highly correlated with the fertility of these sperm. Herein, we administered DBA and BCA, individually and in combination, to determine whether fertility and levels of SP22 on sperm were diminished in an additive fashion. Moreover, we wished to validate an immunoassay for quantitation of SP22. In a dose finding study, animals were exposed by oral gavage daily for 14 days to: BCA alone at 1.6, 4, and 8 mg/kg; DBA at equimolar levels of 2, 5, and 10 mg/kg; and two binary mixtures of 1.6 mg/kg BCA + 2 mg/kg DBA and 4 mg/kg BCA + 5 mg/kg DBA. The ED(50)s for the decrease in SP22 quantified by two-dimensional SDS-PAGE were 7.2 and 4.6 mg/kg for DBA and BCA. The ED(50)s for the decrease in SP22 quantified by ELISA were 8.1 and 5.9 mg/kg for DBA and BCA. The definitive study consisted of 2 and 4 mg/kg DBA, 1.6 and 3.2 mg/kg BCA, and a 2 mg/kg DBA + 1.6 mg/kg BCA mixture. The ED(50)s for decreases in fertility assessed by intrauterine insemination were 3.5 mg/kg and 2.7 mg/kg for DBA and BCA. Immunolocalization of SP22 in spermatocytes and spermatids, as well as on the cytoplasmic droplet and the equatorial segment of luminal sperm, was decreased by the DBA + BCA mixture. The decrease in SP22 in testicular parenchyma was comparable to that observed for sperm extracts. Based on 2D SDS-PAGE, ELISA, or fertility the haloacid-induced decreases in SP22 or fertility were additive or synergistic. The correlation between SP22 levels by ELISA and fertility was r(2) = 0.72 compared to 0.82 for SP22 levels by 2D SDS-PAGE and fertility, validating SP22 quantitation by ELISA.
Novel Cationic Amphiphilic Derivatives from Vernonia Oil:  Synthesis and Self-Aggregation into Bilayer Vesicles, Nanoparticles, and DNA Complexants (2005)

Self-assembling nanostructures were prepared from novel cationic amphiphilic compounds synthesized from vernonia oil, a natural epoxydized triglyceride. The presence of a 12,13-epoxy group on the C18 unsaturated fatty acid, vernolic acid, which is the main constituent of vernonia oil, permitted the synthesis of novel amphiphilic derivatives with a hydrogen-bonding hydroxyl and a cationic headgroup moiety on adjacent carbon atoms. The amphiphiles were prepared in a two-stage synthesis that comprised opening of the epoxy groups with a haloacetic acid, followed by quaternization of the halo group with a tertiary amine containing a C12 aliphatic chain. Intact vernonia oil as the starting material gave a triple-headed cationic amphiphile, containing three vernolic acid derived moieties connected through a glycerol backbone. A single-headed amphiphile with two alkyl chains and a single quaternary ammonium headgroup was synthesized from the methyl ester of vernolic acid as the starting material. The triple-headed derivative could form nonencapsulating structures. Cholesterol was required in the formulation (1:1) to make spherical vesicles that could encapsulate a water-soluble marker. The single-headed derivative, however, formed spherical encapsulating vesicles without cholesterol. TEM, NMR, and FT-IR were used to characterize the vesicles, and molecular structure vs morphology relationships were postulated on the basis of these data. The triple-headed amphiphile also formed a DNA complex that was highly resistant to hydrolysis by DNase. This amphiphile-DNA complex was used as vector for gene transfer in cell culture demonstrating efficient DNA transfection.
JPEG Steganalysis Using Empirical Transition Matrix in Block DCT Domain (2006)

This paper presents a novel steganalysis scheme to effectively attack the JPEG steganographic schemes. The proposed method exploits the correlations between block-DCT coefficients in both intra-block and inter-block sense. We use Markov empirical transition matrices to capture these dependencies. The experimental results demonstrate that the proposed scheme is superior to the existing steganalyzers in attacking OutGuess, F5, and MB1
RESEARCH FOR ROOM-TEMPERATURE MAGNETIC REFRIGERANTS IN RXCE2-XFE17 SERIES (1991)

The Curie temperature Tc of some iron‐based pseudobinary alloys such as RxCe2−xFe17, can be adjusted to room temperature by changing x in the range from 0 to 2 when R is Y or Pr. Therefore the room‐temperature magnetocaloric effects (RTME) can be greatly increased. When R=Pr and x=1.5, the RTME of the alloy reaches the maximum, which is greater than that of Fe by a factor 30 (about one‐half that of Gd). The results are explained in terms of the phase diagram and the critical exponent law.
Two-Particle Bosonic-Fermionic Quantum Walk via Integrated Photonics (2012)

Quantum walk represents one of the most promising resources for the simulation of physical quantum systems, and has also emerged as an alternative to the standard circuit model for quantum computing. Here we investigate how the particle statistics, either bosonic or fermionic, influences a two-particle discrete quantum walk. Such an experiment has been realized by exploiting polarization entanglement to simulate the bunching-antibunching feature of noninteracting bosons and fermions. To this scope a novel three-dimensional geometry for the waveguide circuit is introduced, which allows accurate polarization independent behavior, maintaining remarkable control on both phase and balancement.
Examples of reverse-time migration of single-channel, ground-penetrating radar profiles (1992)

Abstract A single-channel, ground-penetrating radar (GPR) profile portrays a distorted, unfocused image of subsurface structure due to apparent position shifts associated with dipping reflectors and to diffractions from corners and edges. A focused image may be produced from such data by using any of the migration algorithms previously developed for seismic data; we use reverse-time migration based on the scalar wave equation. Field work was performed over a simple stratigraphic soil sequence and a complicated fluvial environment. In the migrated images, reflector continuity is enhanced and the level of detail available for high-resolution interpretation is significantly increased.
Molecular Characterization of the Toxic Cyanobacterium <i>Cylindrospermopsis raciborskii</i> and Design of a Species-Specific PCR (2000)

Cylindrospermopsis raciborskii is a toxic-bloom-forming cyanobacterium that is commonly found in tropical to subtropical climatic regions worldwide, but it is also recognized as a common component of cyanobacterial communities in temperate climates. Genetic profiles of C. raciborskii were examined in 19 cultured isolates originating from geographically diverse regions of Australia and represented by two distinct morphotypes. A 609-bp region of rpoC1, a DNA-dependent RNA polymerase gene, was amplified by PCR from these isolates with cyanobacterium-specific primers. Sequence analysis revealed that all isolates belonged to the same species, including morphotypes with straight or coiled trichomes. Additional rpoC1 gene sequences obtained for a range of cyanobacteria highlighted clustering of C. raciborskii with other heterocyst-producing cyanobacteria (orders Nostocales and Stigonematales). In contrast, randomly amplified polymorphic DNA and short tandemly repeated repetitive sequence profiles revealed a greater level of genetic heterogeneity among C. raciborskii isolates than did rpoC1 gene analysis, and unique band profiles were also found among each of the cyanobacterial genera examined. A PCR test targeting a region of the rpoC1 gene unique to C. raciborskii was developed for the specific identification of C. raciborskii from both purified genomic DNA and environmental samples. The PCR was evaluated with a number of cyanobacterial isolates, but a PCR-positive result was only achieved with C. raciborskii. This method provides an accurate alternative to traditional morphological identification of C. raciborskii.
Staphylococcus aureus Small Colony Variants Are Induced by the Endothelial Cell Intracellular Milieu (1996)

Recent studies have reported that Staphylococcus aureus small colony variants (SCVs) can cause highly persistent infections in humans and in cultured endothelial cells. To understand the process by which SCVs of S. aureus appear in subjects who have not received antibiotic treatment, bovine endothelial cells were coincubated with a wild S. aureus strain for 72 h in the presence of lysostaphin. Intracellular bacteria were harvested and screened for stable SCVs. Intracellular bacteria developed the SCV phenotype at a greater rate than control bacteria not exposed to endothelial cells: The intracellular induction rate was approximately 10(-3) versus a spontaneous rate of <10(-7). This observation suggest that SCVs are induced by the intracellular milieu and suggest a possible mechanism for the intriguing pathophysiology of tissue persistence of staphylococci.
Mechanisms of Drug Sensitization to TRA-8, an Agonistic Death Receptor 5 Antibody, Involve Modulation of the Intrinsic Apoptotic Pathway in Human Breast Cancer Cells (2011)

TRA-8, a monoclonal antibody to death receptor 5 induces apoptosis in various cancer cells; however, the degree of sensitivity varies from highly sensitive to resistant. We have previously shown that resistance to TRA-8 can be reversed by using chemotherapeutic agents, but the mechanism underlying this sensitization was not fully understood. Here, we examined the combination of TRA-8 with doxorubicin or bortezomib in breast cancer cells. In TRA-8-resistant BT-474 and T47D cells, both chemotherapy agents synergistically sensitized cells to TRA-8 cytotoxicity with enhanced activation of apoptosis shown by cleavage of caspases and PARP, reduced Bid, increased proapoptotic Bcl-2 proteins, and increased mitochondrial membrane depolarization. Doxorubicin or bortezomib combined with TRA-8 also reduced Bcl-XL and X-linked inhibitors of apoptosis (XIAP) in treated cells. Furthermore, targeting these proteins with pharmacologic modulators, AT-101, BH3I-2' and AT-406, produced sensitization to TRA-8. TRA-8 combined with AT-101 or BH3I-2', inhibitors of antiapoptotic Bcl-2 proteins, produced synergistic cytotoxicity against ZR-75-1, BT-474, and T47D cells. The IAP-targeting compound, AT-406, was synergistic with TRA-8 in BT-474 cells, and to a lesser extent T47D cells. Activation of the intrinsic apoptotic pathway was a common mechanism associated with sensitization of TRA-8-resistant breast cancer cell lines. Collectively, these studies show that the Bcl-2 and IAP families of proteins are involved in TRA-8 and chemotherapy resistance via their modulation of the intrinsic apoptotic pathway. Targeting these proteins with novel agents sensitized TRA-8-resistant breast cancer cells, suggesting this approach may represent a potent therapeutic strategy in the treatment of breast cancer.
Sacral neuromodulation and peripheral nerve stimulation in patients with anal incontinence: an overview of techniques, complications and troubleshooting (2014)

Sacral neuromodulation (SNM) therapy has revolutionized the management of many forms of anal incontinence, with an expanded use and a medium-term efficacy of 75% overall. This review discusses the technique of SNM therapy, along with its complications and troubleshooting and a discussion of the early data pertaining to peripheral posterior tibial nerve stimulation in incontinent patients. Future work needs to define the predictive factors for neurostimulatory success, along with the likely mechanisms of action of their therapeutic action.
Translation and stability of ovalbumin messenger RNA injected into growing oocytes and fertilized ova of mice (1984)

Growing mouse oocytes and fertilized ova were injected with chicken ovalbumin messenger RNA (mRNAov) and chicken conalbumin mRNA (mRNAcon) and cultured in vitro. Estimation of mRNAov and mRNAcon stability by hybridization of cDNAov and cDNAcon to extracted mRNA from injected oocytes and fertilized ova indicated a half-life of 147 and 366 h in the oocyte and 5 and 3 h in the fertilized ovum respectively. Stability of mRNAov was similar in the fertilized and unfertilized ovum. Oocytes injected with chicken ovalbumin mRNA were also labelled with [3H]leucine and ovalbumin synthesis was measured by immunoprecipitation. The amount of ovalbumin synthesized during the initial 7 h was less than during the period of 18-25 or 66-73 h postinjection. The greatest percentage of ovalbumin to total protein synthesis occurred between 66-73 h. Oocytes secreted 12% of the synthesized ovalbumin during each of the 7 h periods (0-7, 18-25 and 66-73 h) indicating a stable mechanism for secretion throughout the culture period. These studies demonstrate: a dramatic difference in stability of injected mRNA between the growing oocyte and the unfertilized or fertilized ovum, and a gradual increase in the translation of injected mRNA by the growing oocyte during in vitro culture.
The effects of covalent additions of a psoralen on transcription by<i>E. coli</i>RNA polymerase (1987)

Synthetic DNA substrates containing a site-specifically engineered psoralen monoadduct or diadduct were used to characterize the response of the E. coli RNA polymerase elongation complex to these lesions. The psoralen derivative HMT (4'-hydroxymethyl-4,5', 8-trimethylpsoralen) was site specifically placed into two synthetic double-stranded DNA fragments each of which contained an E. coli RNA polymerase promoter at one end. The HMT molecule was attached to the middle of the DNA fragments as either a furan-side monoadduct or an interstrand diadduct. Transcription off the HMT crosslinked DNA templates showed that E. coli RNA polymerase terminated at the HMT diadduct site, i. e., one nucleotide before the modified thymidine residue on the template strand. The furan-side monoadduct when on the template strand also blocked transcription by the polymerase. However, no effect on transcription was observed when the monoadduct was located on the non-template strand.
Cascaded Classification of Gender and Facial Expression using Active Appearance Models. (2006)

This paper presents an approach to recognising the gender and expression of face images by means of active appearance models (AAM). Features extracted by a trained AAM are used to construct support vector machine (SVM) classifiers for 4 elementary emotional states (happy, angry, sad, neutral). These classifiers are arranged into a cascade structure in order to optimise overall recognition performance. Furthermore, it is shown how performance can be further improved by first classifying the gender of the face images using an SVM trained in a similar manner. Both gender-specific expression classification and expression-specific gender classification cascades are considered, with the former yielding better recognition performance. We conclude that there are gender-specific differences in the appearance of facial expressions that can be exploited for automated recognition, and that cascades are an efficient and effective way of performing multi-class recognition of facial expressions.
Pepstatin A alters host cell autophagic machinery and leads to a decrease in influenza A virus production (2011)

Autophagy is a survival mechanism that can take place in cells under metabolic stress and through which cells can recycle waste material. Disturbances in autophagic processes appear to be associated with a number of human pathologies, including viral infections. It has been hypothesized that viruses can subvert autophagy in order to penetrate the host cell and replicate. Because it has been suggested that autophagy is involved in influenza A virus replication, we analyzed the effects of two inhibitors of lysosomal proteases on the cellular control of influenza A virus replication. In particular, we used biochemical and morphological analyses to evaluate the modulation of influenza A/Puerto Rico/8/34 H1N1 virus production in the presence of CA074 and Pepstatin A, inhibitors of cathepsin proteases B and D, respectively. We found that Pepstatin A, but not CA074, significantly hindered influenza virus replication, probably by modulating host cell autophagic/apoptotic responses. These results are of potential interest to provide useful insights into the molecular pathways exploited by the influenza in order to replicate and to identify further cellular factors as targets for the development of innovative antiviral strategies.
Self-adaptive solid-state laser oscillator formed by dynamic gain-grating holograms (1995)

We describe the operation of a self-starting Nd:YAG laser oscillator incorporating a design in which laser oscillation occurs by means of diffraction from spontaneously generated three-dimensional gain gratings produced by spatial hole burning in the Nd:YAG amplifier. The transient onset and spectral selectivity of the gain gratings produce an output with energy of 600 mJ in a 10-ns single-longitudinal-mode pulse at 10 Hz. The self-adaptation of the gain gratings produces compensation of intracavity phase distortion. A transient numerical modeling of the nonlinear resonator gives good agreement with the experimental system and also provides insight into the temporal dynamics of the gain grating.
Biomimetic Nanostructures: Creating a High-Affinity Zinc-Binding Site in a Folded Nonbiological Polymer (2008)

One of the long-term goals in developing advanced biomaterials is to generate protein-like nanostructures and functions from a completely nonnatural polymer. Toward that end, we introduced a high-affinity zinc-binding function into a peptoid (N-substituted glycine polymer) two-helix bundle. Borrowing from well-understood zinc-binding motifs in proteins, thiol and imidazole moieties were positioned within the peptoid such that both helices must align in close proximity to form a binding site. We used fluorescence resonance energy transfer (FRET) reporter groups to measure the change of the distance between the two helical segments and to probe the binding of zinc. We systematically varied the position and number of zinc-binding residues, as well as the sequence and size of the loop that connects the two helical segments. We found that certain peptoid two-helix bundles bind zinc with nanomolar affinities and high selectivity compared to other divalent metal ions. Our work is a significant step toward generating biomimetic nanostructures with enzyme-like functions.