gebhart/patrepeval huggingface dataset:
(R)-3-[(S)-1-Carboxy-5-(4-Piperidyl)pentyl]amino-4-Oxo-2,3,4,5-Tetrahydro-1,5-Benzothiazepine-5-Acetic Acid (CV-5975): A New Potent and Long-Lasting Inhibitor of Angiotensin Converting Enzyme (1988)

The synthetic design and the biological activities of structurally new angiotensin converting enzyme (ACE) inhibitors, (R)-3-amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetic acid derivatives, are described. A number of compounds in this series showed potent ACE inhibitory activity in vitro and in vivo. Structure-activity studies indicated that a piperidyl moiety on the amino group at the 3-position in this series conferred long-lasting ACE inhibitory activity and that the duration of activity depended on the length of the carbon chain in the 1-carboxy-ω)-(4-piperidyl)alkyl group. (R)-3-[(S)-1-carboxy-5-(4-piperidyl) -pentyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzothiazepine-5-acetic acid (CV-5975) was selected as the most promising ACE inhibitor for further studies because of its marked inhibitory activity.
Analysis of RFID Based Positioning Technique Using Received Signal Strength and Directional Antenna (2016)

Automatic robot navigation is being utilized in many industries for the purpose of high speed work delivery. Color follower, fix path follower robots are current solution to this activities but dynamic path configuration is not possible in these robots. Hence new system proposes effective and fully dynamic path follower robots using RFID and directional antenna. Radio Frequency Identification (RFID) system permits automatic identification of objects with RFID tags using radio waves which have been widely used in mobile robot navigation, localization and mapping both in indoor and outdoor environment. This article presents a navigation strategy for autonomous mobile robot using passive RFID system. Proposed robot system is provided with RFID tag functionality which will load tag number and direction instruction. At some turning point, user will put RF tag, this tag will be read by RF reader which is placed on robot. As per direction instruction robot will change the direction and reach to the destination. Also as per the movement, robot will send its GPS location to PC (Personal Computer) which will be displayed on PC. Hence main goal is to provide more reliable and low energy consumption based indoor positioning system which will be achieved using directional antenna.
Blind equalization algorithms with automatic constellation phase recovery for dual-mode CAP-QAM reception (2003)

Some emerging broadband access standards require a receiver that can first blindly start up, then blindly distinguish between carrierless amplitude modulation/phase modulation (CAP) and quadrature amplitude modulation (QAM), and finally decode the signal. The "brute force" solution to this problem would be to implement CAP and QAM receivers in parallel. However, we propose a novel dual-mode receiver based on a single equalizer which accommodates both line codes, yielding significant computational savings. We describe the receiver architecture, blind start-up methods, and line code identification algorithms, as well as computer simulations and a laboratory experiment to verify the theoretical receiver behavior.
Bradykinin Sequesters B2 Bradykinin Receptors and the Receptor-coupled Gα Subunits Gαq and Gαiin Caveolae in DDT1 MF-2 Smooth Muscle Cells (1997)

In this report, we show that the vasoactive peptide agonist bradykinin (BK) when bound to B2 BK receptors on DDT1 MF-2 smooth muscle cells promotes the recruitment and sequestration of the occupied receptors and the receptor-coupled G-protein alpha subunits Galphaq and Galphai in caveolae. Association of ligand receptor complexes and Galpha subunits with caveolae was indicated by their co-enrichment on density gradients with caveolin, a marker protein for caveolae. Caveolin and Galpha subunits were monitored by immunoblotting, whereas receptors were monitored as ligand receptor complexes formed by labeling receptors with the agonist BK or the antagonist NPC17731 prior to cell disruption and caveolae enrichment. These complexes were detected with radioligand and by immunoblotting with BK antibodies. A direct interaction of Galpha subunits with caveolin was also indicated by their co-immunoprecipitation. Immunoelectron microscopy revealed that the enriched caveolin, Galpha subunits, and BK receptor complexes were present in structures of 0.1-0.2 microm. At 4 degrees C, BK and NPC17731 receptor complexes were detected in caveolae, and both complexes were sensitive to acid washing prior to cell disruption and caveolae enrichment. Elevation of the temperature to 37 degrees C increased the amount of BK receptor complexes in caveolae with a maximal response at 10 min (continuous labeling) or 20 min (single-round labeling), and the complexes became acid-resistant. These conditions also increased the amount of Galphaq and Galphai in caveolae with a maximal response at 5-10 min. In contrast, the NPC17731 receptor complexes remained acid-sensitive and dissociated at this temperature, and antagonists did not increase the amount of Galpha subunits in caveolae. These results show that some agonists that act through G-protein-coupled receptors promote the association of their receptors and receptor-coupled Galpha subunits with caveolae.
Molecular Cloning and Expression of a Novel Protease-resistant GH-36 α-Galactosidase from Rhizopus sp. F78 ACCC 30795 (2009)

A 2172-bp full-length gene (aga-F78), encoding a protease-resistant alpha-galactosidase, was cloned from Rhizopus sp. F78 and expressed in Escherichia coli. The deduced amino acid sequence shared highest identity (45.0%) with an alpha-galactosidase of glycoside hydrolase family 36 from Absidia corymbifera. After one step purification with Ni-NTA chelating column, the recombinant Aga-F78 migrated as a single band of ~82 and ~210 kDa on SDS-PAGE and non-denaturing gradient PAGE, respectively, indicating that the native structure of the recombinant Aga-F78 was trimer. Exhibiting the similar properties as the authentic protein, purified recombinant Aga-F78 was optimally active at 50 degrees and pH 4.8, highly pH stable over the pH range 5.0-10.0, more resistant to some cations and proteases, and had wide substrate specificity (pNPG, melidiose, raffinose and stachyose). The recombinant enzyme also showed good hydrolytic ability to soybean meal, releasing galactose of 415.58 microng/g soybean meal. When combined with trypsin, the enzyme remained over 90% degradability to soybean meal. These favorable properties make Aga-F78 a potential candidate for applications in the food and feed industries.
Contact effects on HF loss of CPW high resistivity silicon lines (2002)

This paper shows that the HF losses of CPW lines realized on 5-10 K/spl Omega/cm HRS (High Resistivity Silicon) substrates are strongly affected by the derivative of the I-V curves, ie. HF losses are higher where the I-V characteristic changes most rapidly. As a result the excess HF loss due to choice of quiescent bias voltage can be as high as 0.3-0.4 dB/cm. The implication of the effect is that by proper dc biasing of a CPW line on HRS substrate minimum HF loss can be achieved. This is of importance when active devices are to be biased through line interconnects.
Antigenic properties of human lymphoblastoid interferons (1979)

Most virus-induced human lymphoblastoid interferons examined contained variable proportions of the Le and F antigenic species described for human leukocyte interferon. The F species was not detectable in interferons liberated spontaneously from human lymphoblastoid cells in culture. Lymphoblastoid interferons differed considerably in their interaction with the same anti-interferon serum. Spontaneous interferons required approximately ten times less antibody for neutralization than interferon induced by virus in the same cultures or in Namalva cells. The findings suggest that either spontaneous interferons contain fewer inactive antibody-binding molecules than virus-induced lymphoblastoid interferons or the number and distribution of antibody-combining sites, and possibly other surface properties of the interferon molecule, may be influenced by the manner in which spontaneous and induced interferons egress from the cells.
Development of transfer molding technology for package with die active side partially exposed (2004)

In this paper soft insert molding tcchnology for exposed die packagc is presented. Thc requirements of cxposed die surface to be free of mechanical damage and mold flash create a unique challenge for transfer molding. In addition, the asymmetrical encapsulation presents new reliability risks. Material optimization in the development phase is necessw to obtain a reliable package. Exlensive experimental evaluation showed Ulat good overall molding quality is obtained. This technology is potentially cost effective. There is no evidence of mechanical damage to die due lo molding operation. Flash free surfaces can be aclucved with proper tooling. Molded exposed die package can be reliable with optimized material choices. Further effort is to be made to rcduce the undercut and die surfacc contamination.
Laser beacon tracking for free-space optical communication on small-satellite platforms in low-earth orbit (2015)

Thesis: S.M., Massachusetts Institute of Technology, Department of Aeronautics and Astronautics, 2015.
Meta-analysis of the efficacy of antibiotic therapy in eradicating Helicobacter pylori. (1992)

Despite numerous Helicobacter pylori treatment studies, the optimum regimen(s) for its eradication remain unclear. Our objective was to determine systematically which regimen(s) gave the best pooled eradication rates, by using meta-analysis methodology. A total of 27 studies were identified. Pooled eradication rates for single (18.6%), double (48.2%), and triple therapy (82.3%) were statistically highly different (p < 0.0005). Eradication rates with amoxicillin (23.0%) and bismuth compounds (19.6%) were equivalent. Combined treatment with bismuth+metronidazole was better than bismuth+amoxicillin (55.1% vs. 43.7%, p = 0.049). Triple therapy with bismuth+metronidazole+tetracycline gave a statistically higher eradication rate (94.1%) than bismuth+metronidazole+amoxicillin (73.1%, p < 0.0005). Despite increased side effects with multiple antibiotic regimens, patients tolerated these well, without significant drop-out. The combination of bismuth, metronidazole, and tetracycline gives the best eradication rate, but the optimal doses and duration of treatment have yet to be determined. Further studies are necessary to explore factors such as antibiotic resistance and drug compliance as important factors affecting antibiotic efficacy.
The Dance of Siva: Religion, Art and Poetry in South India (2002)

List of illustrations Acknowledgements Introduction 1. The Nataraja bronze 2. The Cidambaram myth 3. Temple, priests and ritual 4. The Hall of Consciousness, the Heart of the Universe 5. Saiva Siddhanta and Vedanta 6. The Goddess 7. Bhiksatana 8. Bhairava the Terrible and other forms of Siva 9. Saints, dancing girls, ganas and Apasmara 10. Last words Notes Bibliography Index and glossary.
Biochemical properties of regulatory peptides derived from mil proteins (1997)

Biologically active peptides derived from milk proteins are inactive within the sequence of the precursor proteins but can be released by enzymatic proteolysis. Based on structure-activity studies, peptides with a defined bioactivity show common structural features. Moreover, many milk protein-derived peptides reveal multifunctional bioactivities. Bioactive peptide fragments originating from milk proteins should be taken into account as potential modulators of various regulatory processes in the body. Opioid peptides are opioid receptor ligands with agonistic or antagonistic activities. Angiotensin converting enzyme (ACE) inhibitory peptides can exert an antihypertensive effect. Immunomodulating casein peptides have been found to stimulate the proliferation of human lymphocytes and the phagocytic activities of macrophages. Antimicrobial peptides have been shown to kill sensitive microorganisms. Antithrombotic peptides inhibit the fibrinogen binding to a specific receptor region on the platelet surface and also inhibit aggregation of platelets. Casein phosphopeptides can form soluble organophosphate salts and may function as carriers for different minerals, especially calcium. In relation to their mode of action, bioactive peptides may reach target sites (e.g., receptors, enzymes) at the luminal side of the intestinal tract or after absorption, in peripheral organs. The physiological significance of bioactive peptides as exogenous regulatory substances is not yet fully understood. Nevertheless, several bioactive peptides derived from milk proteins have been shown to exert beneficial physiological effects. Milk-derived peptides were already produced on an industrial scale and as a consequence these peptides have been considered for application both as dietary supplements in "functional foods" and as drugs.
Hybrid cell lines constitute a potential reservoir of polarized cells: isolation and study of highly differentiated hepatoma-derived hybrid cells able to form functional bile canaliculi in vitro. (1991)

A large number of hepatoma cell lines has been used to study expression and regulation of liver-specific function. However these cells, even the most differentiated, are morphologically far from hepatocytes. In no case is the typical hepatocyte cell polarity well maintained. Cell hybridization has been used as a potential means for turning on specific genes. From hybrids between well differentiated Fao rat hepatoma cells and WI 38 human fibroblasts, we have attempted to isolate segregated cells that are highly differentiated and polarized. Such cells, detected in aged cultures of only one hybrid (WIF12), were isolated by subcloning. One subclone, WIF12-1 was analyzed. Expression of liver-specific functions extinguished in the original hybrid is restored in all WIF12-1 cells at a very high level, similar to that of hepatocytes and 5-30 times higher that that of parental cells. Moreover human genes coding for liver-specific proteins (albumin, fibrinogen, and alcohol dehydrogenase) are actively expressed. WIF12-1 cells have acquired a polarized phenotype as attested by the presence of bile canaliculi between adjacent cells and by the asymmetrical localization of apical (Mg(2+)-ATPase, gamma-glutamyl transpeptidase) and basolateral membrane markers. The bile canaliculi formed are dynamic and functional structures, characterized by long periods of expansion followed by rapid contractions. The ability to polarize is a general and permanent property of WIF12-1 cells. These cells appear to constitute a valid model for the in vitro study of hepatocyte cell polarity, membrane domain formation and mechanisms of membrane protein sorting.
Clinical heterogeneity associated with deletions in the long arm of chromosome 15: Report of 3 new cases and their possible genetic significance (1987)

Deletions, duplications, and rearrangements of the long arm of chromosome 15 are frequently associated with the clinical diagnosis of the Prader-Willi syndrome. However, a number of other clinical entities have also been associated with similar, if not identical, cytogenetic defects, arguing for clinical heterogeneity associated with abnormalities in this region of chromosome 15. We present 3 patients who all appear to have deletions in 15q11-15q12, such as described for many patients with Prader-Willi syndrome; however, none of these patients has classical clinical features of the Prader-Willi syndrome. The first patient is a child with Williams syndrome, the second, Angelman (Happy Puppet) syndrome, and the third is a child with hypotonia of infancy, obesity, and developmental delay, but who does not meet specific diagnostic criteria for the Prader-Willi syndrome. It is proposed that different molecular abnormalities involving specific points or segments along the long arm of chromosome 15 might account for the clinical diversity seen among these and other patients.
Modelling of a semiconductor optical amplifier based nonlinear optical loop mirror for all-optical regeneration (1995)

Various applications, such as demultiplexing, clock recovery, or all-optical signal regeneration have been demonstrated using nonlinear optical loop mirrors (NOLM), where the nonlinear effects were either spread over the fiber, due to cross phase modulation, or concentrated on a nonlinear element such as a semiconductor optical amplifier (SOA) undergoing cross gain saturation [1]. This paper addresses the last configuration and its application in all-optical regeneration.
Preventive and post‐infection control of <i>Botrytis cinerea</i> in tomato plants by hexanoic acid (2008)

The antifungal activity of hexanoic acid on the phytopathogen Botrytis cinerea was studied. This chemical inhibited both spore germination and mycelial growth in vitro in a concentration‐ and pH‐dependent manner, and stopped spore germination at a very early stage, preventing germ‐tube development. The minimum fungicidal concentration (MFC) for in vitro spore germination was 16 m m . Hexanoic acid also inhibited in vitro mycelial growth of germinated spores at an MFC of 12 m m . Studies performed to characterize the mechanisms underlying the antimicrobial effect of hexanoic acid showed that it alters fungal membrane permeability. In addition, hexanoic acid treatment increased the levels of spermine, spermidine, putrescine and cadaverine in B. cinerea mycelia. Spray application of hexanoic acid at fungicidal concentrations on 4‐week‐old tomato plants prior to fungal inoculation reduced necrosis diameter by approximately 60%. Application of the same hexanoic acid concentrations on previously infected plants reduced further necrosis expansion by around 30%. The results suggest that this chemical acts as a preventive and curative fungicide. Interestingly, treatments with hexanoic acid at concentrations below the MFC in hydroponic solution prior to fungal inoculation significantly reduced necrosis area. These results suggest an inducer effect of plant responses for hexanoic acid treatments at these concentrations. Hexanoic acid is a good candidate for safe antifungal treatments for the control of B. cinerea , which is responsible for many economic losses on fruits, vegetables and flowers.
Acoustic whispering-gallery modes in optomechanical shells (2012)

We numerically calculate the forms and frequencies of mechanical whispering-gallery modes in silica shells. Such modes were recently experimentally observed in water-filled optomechanical resonators, which constitute a bridge between optomechanics and microfluidics. We consider the three acoustic mode families of Rayleigh–Lamb waves, longitudinal waves and Love waves. Our study shows that these acoustic modes have rich velocity dispersion characteristics and can create considerable deformation of the inner surface of the shells. In this manner, a novel optomechanical interaction may be facilitated between fluids and light.
Systems reliability and availability prediction and comparison with field data for a mid-range computer (2002)

The author describes reliability and availability prediction based on systems evaluation through Markov modelling and comparison of results with field observations of a sample of mid-range computer systems with mirrored disks. The system uses single level architecture with disk striping. Reliability evaluation revealed that, with mirroring, the disk system and the intermediate FRUs (field replaceable units) between the disk system and the processor will be duplicated. Hence, system failures would be limited to the processor which is not duplicated. The validity of the prediction was checked by field monitoring of the performance of four systems with mirrored disks. Good agreement between the predicted and observed reliability and availability estimates was observed reliability and availability estimates was observed for the system hardware. As a result of disk monitoring the disk system is totally fault tolerant.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Epitope mapping of hemagglutinating adhesion HA-Ag2 of Bacteroides (Porphyromonas) gingivalis (1992)

Thirteen monoclonal antibodies (MAbs) directed against hemagglutinating adhesion HA-Ag2 of Bacteroides (Porphyromonas) gingivalis were produced by immunizing mice with the relevant immunoprecipitate from crossed immunoelectrophoresis (CIE). Crossed immuno-affinoelectrophoresis and hemagglutination experiments confirmed that our MAbs recognized a molecule able to bind erythrocytes and involved in the hemagglutination process. In immunoelectron microscopy, these MAbs labelled amorphous material as novel cell-bound appendages distinct from fimbriae. CIE experiments allowed differentiation of the MAbs according to reactivity with immunoprecipitates Ag2, Ag8a, and Ag8c, which define HA-Ag2. The epitopes recognized by nine MAbs were mapped on three main antigenic domains (I, II, and III) by competition experiments and further grouped according to chemical composition and distribution on CIE immunoprecipitate. Domain I, defined by two MAbs, comprises an epitope with protein and carbohydrate determinants and distributed on Ag2 only. Epitopes of domain IIA, defined by four MAbs, are distributed on Ag8a, Ag8c, and Ag2 and are essentially composed of protein determinants but also have carbohydrate determinants that enhance the binding of the MAbs but are not essential. Epitopes of domain IIB, defined by two MAbs, and of domain III, defined by a single MAb, have a composition similar to that of domain IIA epitopes but are distributed on Ag8a and Ag8c only. A competition enzyme-linked immunosorbent assay with serum from normal subjects and patients with periodontitis suggested that domain I is more immunogenic than domain II.
Eradication of Johne's Disease from a Heavily Infected Herd in 12 Months (1998)

The goal of the project was to demonstrate that it is FEASIBLE to eradicate paratuberculosis, and that it can be done fairly quickly. The project was not intended to evaluate the most cost-effective strategy for Johne's eradication. Conscientious implementation of husbandry changes to limit spread of M. paratuberculosis infection coupled with accurate record keeping, and a testing program to identify the infected cows for culling should lead to control and eventual elimination of paratuberculosis from most dairy herds.
The design and implementation of SPIRIT: a spatially aware search engine for information retrieval on the Internet (2007)

Much of the information stored on the web contains geographical context, but current search engines treat such context in the same way as all other content. In this paper we describe the design, implementation and evaluation of a spatially aware search engine which is capable of handling queries in the form of the triplet of ⟨theme⟩⟨spatial relationship⟩⟨location⟩. The process of identifying geographic references in documents and assigning appropriate footprints to documents, to be stored together with document terms in an appropriate indexing structure allowing real‐time search, is described. Methods allowing users to query and explore results which have been relevance‐ranked in terms of both thematic and spatial relevance have been implanted and a usability study indicates that users are happy with the range of spatial relationships available and intuitively understand how to use such a search engine. Normalised precision for 38 queries, containing four types of spatial relationships, is significantly higher (p<0.001) for searches exploiting spatial information than pure text search.
Full-Length Genome Sequence of Porcine Deltacoronavirus Strain USA/IA/2014/8734 (2014)

ABSTRACT Porcine deltacoronavirus (PDCoV) was detected in feces from diarrheic sows during an epidemic of acute and transmissible diarrhea. No transmissible gastroenteritis virus or porcine epidemic diarrhea virus was detected. The PDCoV USA/IA/2014/8734 from the herd was sequenced for full-length genomic RNA to further characterize PDCoV in U.S. swine.