gebhart/patrepeval huggingface dataset:
The effect of corticosteroids upon murine B cells in vivo and in vitro as determined in the LPS-culture system. (1987)

The influence of the synthetic corticosteroid dexamethasone sodium phosphate (DEXA) upon mouse B cells was studied. This was done by in vivo treatment of mice with a single or multiple injection of DEXA, and by culturing murine spleen cells and bone marrow cells in vitro in the presence of different concentrations of DEXA. The effect of DEXA on the B-cell compartment was assayed by polyclonal stimulation of the B cells by Escherichia coli lipopolysaccharide (LPS) in vitro and subsequent measurement of the Ig-secreting cell response in the protein A plaque assay. DEXA treatment could greatly reduce the number of B cells in the spleen, but the bone marrow B-cell compartment was quite resistant to DEXA. The in vitro LPS-induced IgM response of the residual B cells from both spleen and bone marrow and their capacity to switch from IgM to IgG and IgA secretion were not affected. These data indicate that DEXA can decrease the total number of B cells but not the functional capacity of the residual LPS-reactive B cells. This was confirmed at the clonal level by limiting dilution culture experiments. The contrasting effects of DEXA on splenic and bone marrow B cells was also found when the cells were exposed to the drug in vitro. It was found that 10(-8) M DEXA in vitro reduced the response of splenic B cells to LPS by more than 80%, while a similar reduction of the response by bone marrow B cells required a 1000-fold higher concentration.
The Heat of Formation of Krypton Difluoride<sup>1</sup> (1966)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Heat of Formation of Krypton Difluoride1Stuart R. GunnCite this: J. Am. Chem. Soc. 1966, 88, 24, 5924Publication Date (Print):December 1, 1966Publication History Published online1 May 2002Published inissue 1 December 1966https://pubs.acs.org/doi/10.1021/ja00976a037https://doi.org/10.1021/ja00976a037research-articleACS PublicationsRequest reuse permissionsArticle Views53Altmetric-Citations9LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Diagnostic and prognostic use of bispectral index in coma, vegetative state and related disorders (2008)

EEG-BIS recording is an interesting additional method to help in the diagnosis as well as in the prognosis of severely brain injured patients recovering from coma.
An innovative machine for automated cutting of fish (1997)

The "Iron Butcher", which is commonly used in the fish-processing industry for head cutting of fish, is known to be inefficient and wasteful, and the resulting product quality may be unacceptable for high-end markets. This paper describes an innovative machine for fish cutting that has been developed by us, employing advanced sensor technology, improved control techniques, and properly designed and integrated components. The new machine eliminates many of the drawbacks of the "Iron Butcher". The components of the machine, their design, and selection are described. Integration of the components, and development and operation of the overall system are presented. An economic analysis is given, in order to indicate the cost advantages of the new machine in comparison with the "Iron Butcher".
Miniaturized Battery‐Free Wireless Systems for Wearable Pulse Oximetry (2016)

Development of unconventional technologies for wireless collection, storage and analysis of quantitative, clinically relevant information on physiological status is of growing interest. Soft, biocompatible systems are widely regarded as important because they facilitate mounting on external (e.g. skin) and internal (e.g. heart, brain) surfaces of the body. Ultra-miniaturized, lightweight and battery-free devices have the potential to establish complementary options in bio-integration, where chronic interfaces (i.e. months) are possible on hard surfaces such as the fingernails and the teeth, with negligible risk for irritation or discomfort. Here we report materials and device concepts for flexible platforms that incorporate advanced optoelectronic functionality for applications in wireless capture and transmission of photoplethysmograms, including quantitative information on blood oxygenation, heart rate and heart rate variability. Specifically, reflectance pulse oximetry in conjunction with near-field communication (NFC) capabilities enables operation in thin, miniaturized flexible devices. Studies of the material aspects associated with the body interface, together with investigations of the radio frequency characteristics, the optoelectronic data acquisition approaches and the analysis methods capture all of the relevant engineering considerations. Demonstrations of operation on various locations of the body and quantitative comparisons to clinical gold standards establish the versatility and the measurement accuracy of these systems, respectively.
Interface molecular engineering of single-walled carbon nanotube/epoxy composites (2011)

Dispersion of large single-walled carbon nanotube (SWCNT) bundles into individual nanotubes or small bundles and thus strengthening of the nanotube/matrix interfacial interaction are prerequisites for taking full advantage of the remarkable multifunctional properties of SWCNTs in various carbon nanotube-based composites. Noncovalent functionalization of SWCNTs is an attractive option to simultaneously achieve these conditions. Toward this end, three reactive amino-containing pyrene derivatives (AmPys) with various spacer chain lengths were synthesized. One with the longest spacer length (12 methylene units, AmPy-12) shows the highest functionalization efficiency for SWCNTs in terms of dispersibility. Systematic characterization on a SWCNT/AmPy-12 hybrid suggests that ca. 10 wt% of AmPy-12 is strongly adsorbed on SWCNTs through π–π interactions, making them steadily dispersed into individual ones and/or small bundles without noticeable change in their electronic structure. AmPy-12-functionalized SWCNTs were then used for the preparation of epoxy composites. Since the SWCNT/epoxy interface was well engineered at a molecular level by application of AmPy-12, which interacts noncovalently with SWCNT but bonds chemically to the epoxy matrix, the composite with only 0.3 wt% SWCNTs displays an increase of 54% and 27% in tensile strength and Young's modulus, respectively, over neat resin. A low electrical percolation threshold of 0.1 wt% SWCNTs and improved thermal properties were also observed.
Parameter space for magnetized fuel targets in inertial confinement fusion (1983)

A simple, zero-dimensional model describing the temporal behaviour of an imploding-shell, magnetized fuel inertial confinement fusion target is formulated. The model includes effects not normally considered in inertial confinement fusion such as magnetic back-pressure on the imploding shell, magnetic reduction of thermal conductivity, magnetic diffusion, and Ohmic heating. The model is simple enough to permit a survey of the parameter space available for magnetized fuel by computing the behaviour of thousands of targets. The survey predicts the existence of a totally new region in parameter space where significant thermonuclear fuel burn-up can occur. The new region is characterized by very low fuel densities, very low implosion velocities, and,most important, driver requirements reduced by several orders of magnitude, suggesting that "break-even" experiments may be possible with existing inertial confinement fusion drivers. The computed results are in reasonable agreement with more complete two-dimensional magnetohydrodynamic simulations.
Performance of 3 x 3 couplers in fiber optic sensor systems (1994)

The performance of fiber optic interferometers constructed with 3x3 couplers is investigated. Deviations from ideal behavior are noted at low fringe visibilities. The use of Faraday mirrors in a 3x3 Michelson configuration is shown to eliminate these effects.
Luminol chemiluminescence: Chemistry, excitation, emitter (1990)

The mechanism of luminol chemiluminescence is a special case of nucleophilic addition to carbonyl compounds. The breakdown of the key intermediate, an alpha hydroxy hydroperoxide, produces a peracid ortho to an acyl diazene group. After intramolecular addition of the peracid, the energy from nitrogen expulsion is utilized in the formation of an anti-aromatic endoperoxide. Rupture along the O,O bond leaves a substantial part of the ensuing phthalate in its excited state. The emitter is shown to be a mono-protonated phthalate unaccessible by photoexcitation. The dark reaction is a concerted decomposition of the alpha hydroxy hydroperoxidixe to yield ground-state phthalate.
Validation of an Off-Eye Contact Lens Shack-Hartmann Wavefront Aberrometer (2008)

The instrument demonstrates good accuracy and repeatability in measuring second-, third-, and fourth-order aberrations of contact lenses and provides the industry with an instrument for evaluating the ex vivo optical characteristics of contact lenses.
Empirical Equation for Calculating the Density of Oxide Glasses (2009)

The density of oxide glass including silicate, borate, phosphate, tellurite, and germanate glasses were measured using the Archimedes method. On the assumption that the ionic packing ratio is approximately a constant independent of chemical composition, an empirical equation for estimating the density from chemical composition was proposed. The calculated values are in reasonable agreement with the corresponding measured ones.
Multiple interaction of fructose 1,6-bisphosphate and other effectors on phosphoenolpyruvate carboxylase from Brevibacterium flavum and its aspartate-producing mutant. (1986)

Phosphoenolpyruvate (PEP) carboxylase purified from Brevibacterium flavum was specifically activated by fructose 1, 6-bisphosphate (FBP). The other intermediates of sugar metabolism or their structural analogues did not influence the activity. FBP decreased the apparent Km for PEP but did not affect that for another substrate, bicarbonate, or the apparent maximum velocity for PEP. The dissociation constants for FBP from enzyme-FBP and enzyme-PEP-FBP complex were 63 and 32 μM, respectively, being almost equivalent to those for acetyl-CoA. Synergistic activation by FBP and acetyl-CoA was not observed with the B. flavum enzyme, unlike the Escherichia coli enzyme. FBP, like acetyl-CoA, was kinetically competitive with aspartate. With respect to another feedback inhibitor, 2-oxoglutarate, acetyl-CoA was non-competitive, whereas FBP was of mixed-type, i.e., FBP but not acetyl-CoA prevented 2-oxoglutarate from binding to the enzyme to a certain extent. Homotropic cooperativity was observed only with FBP but not with acetyl-CoA in the absence of inhibitors. Cooperativities of FBP and acetyl-CoA were increased by aspartate but not by 2-oxoglutarate. In the aspartate-overproducing mutant enzyme, the Michaelis constant for PEP was decreased, whereas the inhibitor constant for aspartate with or without simultaneous addition of 2-oxoglutarate and the activator constants for FBP and acetyl-CoA were increased. The decreased Michaelis constant for PEP was comparable to the apparent Km of the wild-type enzyme for PEP in the presence of the saturated concentration of FBP, and would result in a further decrease in the affinity of the mutant enzyme for aspartate.
ESD protection in finfet technologies (2011)

Strong dependency on - process technology (SOIFF, bulkFF) - process options (strain, SEG,...) - layout parameters (L <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">G</sub> , W <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">fin</sub> ,...) ESD needs to be considered early during technology development ESD remains very challenging but ESD is no roadblock for the introduction of both SOIFF and bulkFF!
Red cell hemolysis during processing and storage (2007)

Hemolysis of the red cells increases due to processing and during storage and is maximum during the first week. Adequate process control and proper storage facilities should be ensured to minimize the hemolysis of red cells during processing and storage.
Translational diffusion measured by PFG‐NMR on full length and fragments of the Alzheimer Aβ(1–40) peptide. Determination of hydrodynamic radii of random coil peptides of varying length (2002)

Abstract PFG‐NMR methods were used to measure the translational diffusion coefficients for the Aβ peptide involved in Alzheimer's disease and also for a series of fragments of this peptide. The peptides ranged from a pentamer to the full length Aβ(1–40). They were studied at 25° C and physiological pH in aqueous solution. The measured diffusion coefficients, including those of known monomeric peptides, were fitted without systematic deviations to a scaling law function of the molecular mass. We concluded that under these conditions Aβ(1–40) is in monomeric form. From the diffusion coefficient data, hydrodynamic radii r H were evaluated for the peptides. When combining our results on non‐ or weakly structured peptides with previously reported results on denatured proteins, we found that the hydrodynamic radii for the combined dataset could be well described by the same scaling law relating them to the molecular weight. The same law would even encompass data on single amino acids and di‐ and tripeptides measured by classical methods. From the above‐mentioned experimental data, scaling law parameters were determined. The relation between the measured hydrodynamic radius ( r H ) and the molecular weight of the polypeptide chain ( M r ) for amino acids, peptides and denatured proteins is r H = 0.27M r 0.50 Å. There is a remarkably good fit to this function for the measured hydrodynamic radii in a large range, almost three orders of magnitude, of molecular weights. The numerical value of the exponent, 0.5, is an indication that these polymers behave as Gaussian chains. Copyright © 2002 John Wiley & Sons, Ltd.
Absolute Efficiency Calibration of a Beta-Gamma Detector (2013)

Identification and quantification of nuclear events such as the Fukushima reactor failure and nuclear explosions rely heavily on the accurate measurement of radioxenon releases. One radioxenon detection method depends on detecting beta-gamma coincident events paired with a stable xenon measurement to determine the concentration of a plume. Like all measurements, the beta-gamma method relies on knowing the detection efficiency for each isotope measured. Several methods are commonly used to characterize the detection efficiency for a beta-gamma detector. The first and easiest method is to use a traceable (e.g., NIST) gamma standard to determine the detection efficiency. A second method determines the detection efficiencies relative to an already characterized detector. Finally, a potentially more accurate method is to use isotopes that the system is intended to measure and the form the system is intended to measure to perform an absolute efficiency calibration; in the case of a beta-gamma detector, this relies on radioxenon gas samples. The complication of the first method is it focuses only on the gamma detectors and does not offer a solution for determining the beta efficiency. The second method listed is not similarly constrained, however it relies on another detector to have a well-known efficiency calibration. The final method using actual radioxenon samples to make an absolute efficiency determination is the most desirable, but until recently, it was not possible to produce all four isotopically pure radioxenon isotopes. The production, by University of Texas (UT), of isotopically pure radioxenon has allowed the beta-gamma detectors to be calibrated using the absolute efficiency method. The first four radioxenon isotope calibration will be discussed in this paper.
Sequence and primer independent stochastic heterogeneity in PCR amplification efficiency revealed by single molecule barcoding (2014)

The polymerase chain reaction (PCR) is one of the most widely used techniques in molecular biology. In combination with High Throughput Sequencing (HTS), PCR is widely used to quantify transcript abundance for RNA-seq and especially in the context of analysis of T cell and B cell receptor repertoires. In this study, we combine molecular DNA barcoding with HTS to quantify PCR output from individual target molecules. Our results demonstrate that the PCR process exhibits very significant unexpected heterogeneity, which is independent of the sequence of the primers or target, and independent of bulk experimental conditions. The mechanistic origin of this heterogeneity is not clear, but simulations suggest that it must derive from inherited differences between different DNA molecules within the reaction. The results illustrate that single molecule barcoding is important in order to derive reproducible quantitative results from any protocol which combines PCR with HTS.
Rifampicin-Independent Interactions between the Pregnane X Receptor Ligand Binding Domain and Peptide Fragments of Coactivator and Corepressor Proteins (2011)

The pregnane X receptor (PXR), a member of the nuclear receptor superfamily, regulates the expression of drug-metabolizing enzymes in a ligand-dependent manner. The conventional view of nuclear receptor action is that ligand binding enhances the receptor's affinity for coactivator proteins, while decreasing its affinity for corepressors. To date, however, no known rigorous biophysical studies have been conducted to investigate the interaction among PXR, its coregulators, and ligands. In this work, steady-state total internal reflection fluorescence microscopy (TIRFM) and total internal reflection with fluorescence recovery after photobleaching were used to measure the thermodynamics and kinetics of the interaction between the PXR ligand binding domain and a peptide fragment of the steroid receptor coactivator-1 (SRC-1) in the presence and absence of the established PXR agonist, rifampicin. Equilibrium dissociation and dissociation rate constants of ~5 μM and ~2 s(-1), respectively, were obtained in the presence and absence of rifampicin, indicating that the ligand does not enhance the affinity of the PXR and SRC-1 fragments. Additionally, TIRFM was used to examine the interaction between PXR and a peptide fragment of the corepressor protein, the silencing mediator for retinoid and thyroid receptors (SMRT). An equilibrium dissociation constant of ~70 μM was obtained for SMRT in the presence and absence of rifampicin. These results strongly suggest that the mechanism of ligand-dependent activation in PXR differs significantly from that seen in many other nuclear receptors.
Integrating Information and Knowledge with Software Agents (2000)

This paper describes research and development activities for integrating distributed information and knowledge with software agent technology. First, we give an overview of our software agent architecture. Then, we describe the application of our software agent technology in a system that integrates distributed databases and in a document-oriented information integration system which integrates search engines. Our agent-based approach is suitable for dynamic information sources.
Histological and radiographic comparison of allograft substitutes using a continuous delivery model in segmental defects. (2007)

Demineralized bone matrix (DBM) has been shown to possess osteoinductive capability and one of the specific bone morphogenetic proteins (BMPs) found within DBM that has been attributed with this osteoinductive ability is BMP-7, also known as osteogenic protein-1 (OP-1). The specific aims of this study were (1) to compare the treatment of segmental bone defects with OP-l and DBM in a rat femur model and (2) to determine the effects of the two treatments given at high and low doses via sustained release drug delivery. Animals in Group 1 acted as the control and Group 2 had a created segmental defect with plating and placement of a calcined tricalcium phosphate lysine (TCPL) capsule containing antibiotic (sham). Group 3 and 4 animals had a created segmental defect and received a TCPL carrier containing antibiotic along with DBM or OP-1, respectively. After 4 weeks post-implantation, animals were sacrificed before the retrieval of the bone. The femora were analyzed radiographically and histologically for bone growth. Analysis of the gross specimens showed considerable bone regeneration at low and high doses for both DBM and OP-1 when compared to the shams. At low levels bone regeneration between DBM and OP-1 was very similar. However, at high doses, OP-1 was shown to cause bone overgrowth with a greater curvature and an increased thickness of the distal and proximal ends of the femur. The stained slides showed the defects treated with DBM and OP-1 to be bridged with lamellar and woven bone that was continuous with the original bone. Histologically, the experimental femora demonstrated natural remodeling processes with new osteons and angiogenesis.
Device-Agnostic Log Anomaly Classification with Partial Labels (2018)

Anomaly classification, i.e., detecting whether a network device is anomalous and determining its anomaly category if yes, plays a crucial role in troubleshooting. Compared to KPI curves, device logs contain too much more valuable information for anomaly classification. However, the regular expression based anomaly classification techniques cannot tackle the challenges lying in log anomaly classification. We propose LogClass, a data-driven framework to detect and classify anomalies based on device logs. LogClass combines a word representation method and the PU learning model to construct device-agnostic vocabulary with partial labels. We evaluate LogClass on tens of millions of switch logs collected from several real-world datacenters owned by a top global search engine. Our results show that LogClass achieves 99.515% F1 score in anomalous log detection, 95.32% Macro-F1 and 99.74% Micro-F1 in anomalous log classification in a computationally efficient manner.
Activity of Chitosans in combination with antibiotics in <i>Pseudomonas aeruginosa</i> (2009)

Chitosan and its derivative water soluble Chitosan oligosaccharide are used in a variety of applications in pharmaceutical preparations. In this study, 2 wild (ATCC 15729 and PAO1) and 2 mutant strains (PT121 and PT149) of P. aeruginosa are investigated for drug-drug interactions in vitro. 10 antimicrobial agents (antibiotics) are combined with different degree of deacetylated Chitosans and Chitosan oligosaccharide. All the chitosans show synergistic activity with sulfamethoxazole, a sulfonamide antimicrobial agent. It is interesting to observe that the MIC value for the MexEF-OprN overexpressing mutant strain of P. aeruginosa is 5 fold higher than the other strains under investigation suggesting a possible role of this efflux pump in Sulfamethoxazole efflux. The findings suggest on the use of chitosans as enhancing agent in combination with antibiotics in pharmaceutical preparations.