gebhart/patrepeval huggingface dataset:
The Hexapeptide Inhibitor of Galβ1,3GalNAc-specific α2,3-Sialyltransferase as a Generic Inhibitor of Sialyltransferases (2002)

The mammalian Galbeta1,3GalNAc-specific alpha2,3-sialyltransferase (ST3Gal I) was expressed as a secreted glycoprotein in High Five (Trichoplusia ni) cells. Using this recombinant ST3Gal I, we screened the synthetic hexapeptide combinatorial library to explore a sialyltransferase inhibitor. We found that the hexapeptide, NH(2)-GNWWWW, exhibited the most strong inhibition of ST3Gal I among five different hexapeptides that were finally selected. The kinetic analysis of ST3Gal I inhibition demonstrated that this hexapeptide could act as a competitive inhibitor (K(i) = 1.1 microm) on CMP-NeuAc binding to the enzyme. Moreover, the hexapeptide was shown to strongly inhibit both N-glycan-specific alpha2,3- and alpha2,6-sialyltranferase in vitro, suggesting that this peptide may inhibit the broad range of sialyltransferases regardless of their linkage specificity. The inhibitory activity in vivo was investigated by RCA-I lectin blot analyses and by metabolic d-[6-(3)H]GlcNH(2) radiolabeling analyses of N- and O-linked oligosaccharides in Chines hamster ovary cells. Our results demonstrate that the hexapeptide can act as a generic inhibitor of the N- and O-glycan-specific sialyltransferases in mammalian cells, which results in the significantly reduced NeuAc expression on cellular glycoproteins in vivo.
Femtosecond laser microstructuring and bioactive nanocoating of titanium surfaces in relation to chondrocyte growth (2010)

Introduction: Titanium implants can be regarded as the current gold standard for restoration of sound transmission in the middle ear following destruction of the ossicular chain by chronic inflammation. Many efforts have been made to improve prosthesis design, while less attention had been given to the role of the interface. We present a study on chemical nanocoating on microstructured titanium contact surface with bioactive protein. Materials and Methods: Titanium samples of 5mm diameter and 0,25mm thickness were structured by means of a Ti:Sapphire femtosecond laser operating at 970nm with parallel lines of 5μm depth, 5μm width and 10μm inter-groove distance. In addition, various nanolayers were applied to titanium samples by aminosilanization, to which Star-Polyethylene glycole (Star-PEG) molecules plus biomarkers (e.g. RGD peptide sequence) were linked. Results: Chondrocytes could be cultured on microstructured surfaces without reduced rate of vital / dead cells compared to native surfaces. Chondrocytes also showed contact guidance by growing along ridges particularly on 5μm lines. On nanocoated titanium samples, first results showed a strong effect of Star-PEG suppressing unspecific protein absorption, while RGD peptide sequence did not promote chondrocyte cell growth. Discussion: According to these results, the idea of promoting cell growth on titanium prosthesis contact surfaces compared to non-contact surfaces (e.g. prosthesis shaft) by nanocoating is practicable. However, relative selectivity induced by microstructures for growth of chondrocytes compared to fibrocytes is subject to further evaluation.
A structural model for binaural sound synthesis (1998)

A structural model is presented for synthesizing binaural sound from a monaural source. The model produces well-controlled vertical as well as horizontal effects. The model is based on a simplified time-domain description of the physics of wave propagation and diffraction. The components of the model have a one-to-one correspondence with the physical sources of sound diffraction, delay, and reflection. The simplicity of the model permits efficient implementation in DSP hardware, and thus facilitates real-time operation. Additionally, the parameters in the model can be adjusted to fit a particular individual's characteristics, thereby producing individualized head-related transfer functions. Experimental tests verify the perceptual effectiveness of the approach.
The breast cancer-associated stromelysin-3 gene is expressed during mouse mammary gland apoptosis. (1992)

We have cloned from a mouse placenta cDNA library a mouse homologue of the human stromelysin-3 (ST3) cDNA, which codes for a putative matrix metalloproteinase expressed in breast carcinomas. The ST3 protein is well conserved between humans and mice, and the pattern of ST3 gene expression is similar in both species, and shows expression in the placenta, in the uterus, and during limb bud morphogenesis. We show that the ST3 gene can also be expressed in the normal mouse mammary gland. ST3 gene expression was not detected during mammary growth, neither in virgin nor in pregnant mice, but was specifically observed during postlactating involution of the gland, an apoptotic process associated with intense extracellular matrix remodeling. ST3 transcripts were found in fibroblasts immediately surrounding degenerative ducts, suggesting that ST3 gene expression may be associated with the basement membrane dissolution, which occurs during mammary gland involution. Since the ST3 gene is also specifically expressed in fibroblastic cells surrounding invasive neoplastic cells of breast carcinomas, we suggest that ST3 is implicated in extracellular matrix remodeling processes common to mammary apoptosis and breast cancer progression.
Energy-Aware Mobile Service Overlays: Cooperative Dynamic Power Management in Distributed Mobile Systems (2007)

With their increasingly powerful computational resources and high-speed wireless communications, future mobile systems will have the ability to run sophisticated applications on collections of cooperative end devices. Mobility, however, requires dynamic management of these platforms' distributed resources, and such management can also be used to meet application quality requirements and prolong application lifetimes, the latter by best using available energy resources. This paper presents energy-aware mobile service overlays (MSOs), a set of mechanisms and associated policies for running mobile applications across multiple, cooperating machines while actively performing power management to extend system usability lifetimes. MSO policies manage energy consumption by (i) allocating application components to available nodes based upon their current energy capacities and resource availabilities, (ii) monitoring for, and responding to changes in energy and resource characteristics, and (iii) dynamically exploiting energy-performance tradeoffs in over provisioned situations. Coupled with mobility, such cooperation enables multiple mobile platforms to bring their joint resources to bear on complex application tasks, providing significant benefits to application lifetimes and performance. Evaluations of MSOs on a MANET computing testbed indicate an extension in system lifetime of up to 10% for an example application.
Microwave sensing for meat and fish structure evaluation (2007)

Monitoring changes in muscle structure during the ageing of bovine meat and quality loss due to fish freezing are major industrial challenges. During ageing, bovine muscle becomes tender through muscle fibre deterioration, and full control of this process is essential. Conversely, degradation of fish muscle, often due to long storage or a freezing cycle, lowers quality. To improve competitiveness, and to respond to consumer quality demand, muscle structure needs to be evaluated in-line. We present here a polarimetric microwave method (10–24 GHz) consisting in free space and contact reflection coefficient measurements using a horn antenna and rectangular probes, respectively. This method is based on the measurement of dielectric properties of tissues parallel and perpendicular to muscle fibre directions. The dielectric properties of structured tissues such as muscles are anisotropic, but during processing structural disorganization reduces this anisotropy. The method is illustrated by the discrimination of fresh and frozen-thawed fish fillets and by monitoring of meat ageing.
Identification of a Functional Type VI Secretion System in Campylobacter jejuni Conferring Capsule Polysaccharide Sensitive Cytotoxicity (2013)

The pathogen Campylobacter jejuni is the principal cause of bacterial food-borne infections. The mechanism(s) that contribute to bacterial survival and disease are still poorly understood. In other bacterial species, type VI secretion systems (T6SS) are increasingly recognized to contribute to bacterial pathogenesis by toxic effects on host cells or competing bacterial species. Here we report the presence of a functional Type VI secretion system in C. jejuni. Proteome and genetic analyses revealed that C. jejuni strain 108 contains a 17-kb T6SS gene cluster consisting of 13 T6SS-conserved genes, including the T6SS hallmark genes hcp and vgrG. The cluster lacks an ortholog of the ClpV ATPase considered important for T6SS function. The sequence and organization of the C. jejuni T6SS genes resemble those of the T6SS located on the HHGI1 pathogenicity island of Helicobacter hepaticus. The C. jejuni T6SS is integrated into the earlier acquired Campylobacter integrated element CJIE3 and is present in about 10% of C. jejuni isolates including several isolates derived from patients with the rare clinical feature of C. jejuni bacteremia. Targeted mutagenesis of C. jejuni T6SS genes revealed T6SS-dependent secretion of the Hcp needle protein into the culture supernatant. Infection assays provided evidence that the C. jejuni T6SS confers contact-dependent cytotoxicity towards red blood cells but not macrophages. This trait was observed only in a capsule-deficient bacterial phenotype. The unique C. jejuni T6SS phenotype of capsule-sensitive contact-mediated hemolysis represents a novel evolutionary pathway of T6SS in bacteria and expands the repertoire of virulence properties associated with T6SS.
Ultrasound in organic synthesis. 7. Preparation of organozinc reagents and their nickel-catalyzed reactions with .alpha.,.beta.-unsaturated carbonyl compounds (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUltrasound in organic synthesis. 7. Preparation of organozinc reagents and their nickel-catalyzed reactions with .alpha.,.beta.-unsaturated carbonyl compoundsChristian Petrier, Jayne De Souza Barbosa, Claude Dupuy, and Jean Louis LucheCite this: J. Org. Chem. 1985, 50, 26, 5761–5765Publication Date (Print):December 1, 1985Publication History Published online1 May 2002Published inissue 1 December 1985https://pubs.acs.org/doi/10.1021/jo00350a065https://doi.org/10.1021/jo00350a065research-articleACS PublicationsRequest reuse permissionsArticle Views2540Altmetric-Citations91LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
<title>New low-phonon frequency crystals based on rare-earth-doped double halogenides for multiwavelength diode-pumped solid state lasers</title> (2002)

Energy (frequency) of phonons is the main parameter which determines ratio between probabilities of radiative and radiationless transitions in luminescence. Single crystals of double chlorides KPb2C15 and bromides KPb2Br5 , which are formed by heavy ions and have a low energy phonon spectrum (h? <200 and 150 cm-1, respectively), of optical quality were obtained using the Bridgmen-Stockbarger technique. We studied the optical spectra and luminescence kinetics of RE —doped crystals (RE= Pr3+, Nd3+, Tb3+, Ho3+, Er3+ etc). Intensity parameters were determined by the Judd-Ofelt method, radiative and non-radiative transition probabilities were calculated. It was shown that low multiphonon relaxation rate in these crystals together with high values of radiative probabilities leads to the evidence of high intensity luminescence in spectral domain from 360 to 9000 nm. These features make these crystals promising for practical applications as active media for UV, VIS and mid-IR solid state lasers and amplifiers with laser diode pumping.
[Functions of bacteriophage T4 rII genes. Preparatory isolation and several properties of rIIB protein]. (1976)

A method of preparative isolation of membrane rIIB protein from bacteriophage T4 is worked out. Conditions are found to maximal rIIB protein accumulation in membranes of E. coli cells infected with bacteriophage T4. The membrane isolation by ultracentrifugation is substituted with their sedimentation from polyethyleneglycol solutions by low-speed centrifugation. A fraction, enriched with rIIB protein, is obtained using the treatment of cell walls with detergents. Preparative polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate was used for further rIIB protein purification. The method described can be applied for the purification and preparative isolation of memorane and poor-soluble proteins. The problem on location and ufunctioning of rIIB protein is discussed.
Wide-range RF MEMS variable inductor using micro pump actuator (2008)

A novel variable radio-frequency (RF) microelectromechanical system (MEMS) inductor using micro-pump actuator is presented, which inductance is of- nH for GHz applications.The variable inductor has micro-pump fluid conductor as mercury and a spiral inductor fabricated on a Si CMOS chip. Micropump activates the fluid displacement by using passive microvalves. The fluid moves between inter-spires distance and shortening the path length of the current through the structure; leading to reduction of the stored magnetic energy, and hence the inductance. To reduce the substrate loss and thus increase the quality factor of the inductor, the silicon underneath spiral inductor is etched away. Electrical performance of variable inductor is analyzed using a 3D electromagnetic simulation tool (Ansoft). At 8.3 GHz, the simulated inductor is continuously varied from 2.22 nH to 0.88 nH, i.e., the variable range is above 100%. The proposed variable inductor is perspective key component for the multi-band RF circuits such as electrically controllable matching circuits and wide tuning range voltage controlled oscillator (VCO).
Protein Kinase A-Dependent Cooperation between GATA and CCAAT/Enhancer-Binding Protein Transcription Factors Regulates Steroidogenic Acute Regulatory Protein Promoter Activity (2002)

Steroidogenic acute regulatory protein (StAR) is an essential cholesterol transporter in steroidogenic tissues. Hormone-induced StAR expression is regulated through the cAMP-dependent pathway involving activation of protein kinase A (PKA). The StAR promoter contains several conserved DNA regulatory elements. These include binding sites for steroidogenic factor 1, CCAAT/enhancer-binding protein (C/EBP), and GATA transcription factors. Although these elements are important for StAR promoter activity, how the various transcription factors that bind these elements cooperate to confer cAMP responsiveness remains poorly understood. As induction of StAR transcription by cAMP in steroidogenic MA-10 cells does not require de novo protein synthesis, this suggests that all essential transcription factors are present and that posttranslational modifications of the factors are involved. We now report that GATA-4 is phosphorylated in MA-10 cells in response to cAMP and in heterologous CV-1 cells, GATA-4 transcriptional activity is stimulated by PKA. Moreover, we show that GATA-4 and C/EBPbeta directly interact in vitro and in vivo and synergistically activate the StAR promoter in CV-1 cells exclusively in the presence of PKA. As PKA-dependent synergy was also observed with other GATA and C/EBP family members, this transcriptional cooperation may contribute to hormone-stimulated StAR expression in all steroidogenic tissues.
Clathrate desalination plant preliminary research study. Water treatment technology program report No. 5 (Final) (1995)

This report presents preliminary research, design, and cost estimates for a clathrate freeze desalination method and system. A clathrate former is injected through the inner pipe of a submerged pipeline to a predetermined ocean depth at which the ocean temperture is less than the clathrate forming temperture. The agent combines with seawater within the annulus of the outer pipe to form a slurry of clathrate ice crystals and brine that is pumped to the surface. The clathrate forming agent may be recovered for reinjection or discarded. The melting of the clathrate ice and the return line of brine water to the ocean provide cold water sources that can be used to cool refrigerant in air conditioning systems in local buildings. The system depicted uses the hydrocarbon HCFC R141b (Dichloromonofluoroethane- CCl2FCH3) as the clathrate forming agent with lesser attention on HCFC R22 (Chlorodifluoromethane - CHClF2) and carbon dioxide (C02).
Si2, SiH3, and HSiO molecules: ESR at 4 K (1985)

ESR spectra of 29Si2, 29SiH3, and H29SiO molecules in argon and/or neon matrices at 4 K have yielded the following new data: Si2 has a zero-field splitting in its ground3Σ−g state of ‖D‖=2.53(1) cm−1 in an argon matrix, which extrapolates to a gas-phase parameter of ‖λ‖=1.35(5) cm−1. The hyperfine interaction constant ‖A⊥(29Si)‖=40(2) MHz. SiH3 has g∥=2.0043(5), g⊥=2.0035(5), ‖Aiso(H)‖=25(3), ‖Aiso(29Si)‖=665(4), and ‖Adip(29Si)‖=166(3) MHz. Probable spin densities are 15% sSi+91% pSi−5% sH with a calculated bond angle of 113° in solid argon. HSiO has been observed for the first time. Its g components and spin distribution are very similar to those of the HCO radical. It is bent with a presumed 2A′ ground state, in accord with unpublished calculations of Bruna and Peyerimhoff and the recently published study of Frenking and Schaefer. The more stable isomer HOSi may have also been detected here.
Augmented antitumor effects of combination therapy with interleukin-12, cisplatin, and tumor necrosis factor-alpha in a murine melanoma model. (1998)

Interleukin-12 (IL-12) has demonstrated antitumor activity in many murine tumor models. However, toxic effects resulting from treatment with IL-12 have been also described. Combining IL-12 with other antineoplastic agents could potentiate its antitumor efficacy and, furthermore, could minimize its toxicity by reducing the doses necessary to achieve the antitumor activity. We examined in a murine melanoma model the efficacy of combination tumor chemo-immunotherapy based on administration of IL-12, cisplatin (CDDP), and tumor necrosis factor-alpha (TNF-alpha). In the current study pairs of: IL-12 + CDDP and IL-12 + TNF-alpha, showed stronger antitumor activity than either agent given alone. Furthermore, combination tumor therapy with IL-12 + CDDP + TNF-alpha was more effective at retarding local tumor growth than either IL-12 + CDDP, IL-12 + TNF-alpha or CDDP + TNF-alpha combination therapies. Our observations indicate that combining of CDDP with IL-12 and IL-12 with TNF-alpha as well as using the triple combination of CDDP, IL-12 and TNF-alpha could be beneficial in tumor therapy.
Chemical and modal composition of dunite from Dun Mountain, Nelson (1959)

Summary A chemical and modal analysis is presented of the type dunite discovered by F. von Hochstetter on Dun Mountain, Nelson.
A review of some aspects of the epidemiology, diagnosis, and control of Mycoplasma hyopneumoniae infections (2001)

Mycoplasma hyopneumoniae (MH), one of the most important swine pathogens, is distributed world wide. Alone, MH is relatively benign,but associated with other pathogens, which is almost always the case in field situations, it may cause serious losses. The combination of porcine reproductive and respiratory syndrome virus and MH is particularly important in respiratory problems of grow-finish pigs. Different strains of MH exist, but their significance remains to be evaluated. Transmission occurs mainly by direct contact and aerosol. Cranioventral lesions of pneumonia are suggestive of MH, but are not pathognomonic. The use of modern production technologies has coincided, to a certain extent, with an upsurge of MH-associated problems. Antimicrobials are more efficacious when used for prevention rather than treatment. Optimal timing for vaccination depends on the age at which pigs will come in contact with the organism and their level of maternal immunity when vaccinated. It is possible to produce MH-negative pigs from infected herds and to eradicate the organism without total depopulation.
Structure, Properties, Functionalization, and Applications of Carbon Nanohorns (2016)

Carbon nanohorns (sometimes also known as nanocones) are conical carbon nanostructures constructed from an sp(2) carbon sheet. Nanohorns require no metal catalyst in their synthesis, and can be produced in industrial quantities. They provide a realistic and useful alternative to carbon nanotubes, and possibly graphene, in a wide range of applications. They also have their own unique behavior due to their specific conical morphology. However, their research and development has been slowed by several factors, notably during synthesis, they aggregate into spherical clusters ∼100 nm in diameter, blocking functionalization and treatment of individual nanocones. This limitation has recently been overcome with a new approach to separating these "dahlia-like" clusters into individual nanocones. In this review, we describe the structure, synthesis, and topology of carbon nanohorns, and provide a detailed review of nanohorn chemistry.
Applying the blockchain technology to promote the development of distributed photovoltaic in China (2018)

Access to energy has increasingly been provided by the Chinese Government via new alternative energy sources known as renewables in recent years. Meanwhile, the development and use of environmentally friendly renewables gradually become the basic requirements for the sustainable development in the future society. The integration of blockchain technology with distributed photovoltaic (PV) energy may break the existing pattern where the production, transportation, distribution, and sales of energy are centralized. This paper first reviews the current overall situation of China's distributed PV and further analyzes the policy environment with respect to the development of distributed PV. On the basis of the analysis of the status quo, the paper then discusses the internalities (strengths and weaknesses) and the externalities (opportunities and threats) that have driven the development of China's distributed PV by illustrating the SWOT analysis. The data structure and characteristics of blockchain are analyzed to identify the application mode of blockchain technology in the distributed PV industry for the first time. Through our research, some conclusions and policy proposals are finally put forward to provide support to the formulation of related policy in the Chinese Government and industry association.