gebhart/patrepeval huggingface dataset:
Carbonic anhydrase in the plasma membranes from leaves of C<sub>3</sub> and C<sub>4</sub> plants (1993)

Plasma membranes were isolated from green leaves of maize ( Zea mays ), spinach ( Spinacia oleracea ), Setaria viridis and wheat ( Triticum aestivum cv. Omase) by aqueous two‐phase partitioning. Carbonic anhydrase activity was detected in these membranes. The activity was inhibited by specific inhibitors for carbonic anhydrase, acetazolamide and ethoxyzolamide. The carbonic anhydrase activity was markedly enhanced by the addition of Triton X‐100 to the plasma membranes. The highest activity was obtained in the presence of 0.015% detergent. The activity was scarcely affected when the plasma membrane vesicles were treated with proteinase K, but largely inactivated by the protease after treating the membranes with Triton X‐100. These results indicate that carbonic anhydrase faces the cytoplasmic side of the membrane since plasma membranes purified by aqueous two‐phase partitioning are tightly sealed vesicles of right side‐out orientation (apoplastic side‐out). With leaves of C 4 plants, 20 to 60% of the total carbonic anhydrase activity was found in the microsomal fraction. By contrast, only 1 to 3% of the activity was found in the microsomal fraction from leaves of C 3 plants. Western blot analysis showed that a polypeptide in the spinach plasma membrane cross‐reacted with an antiserum raised against spinach chloroplast carbonic anhydrase, and that the molecular mass of the plasma membrane enzyme was higher than that of the chloroplast carbonic anhydrase (28 and 26 kDa, respectively). This indicates the presence of different molecular species of carbonic anhydrase in the chloroplast and the plasma membrane.
A robust and fast block motion estimation algorithm (2002)

The authors present a new algorithm for fast block motion estimation in video coding applications. In this new algorithm, general characteristics of the block motion field are explored and related to the block motion vector statistics in the search window. As such, they present a way to design the optimal checking point pattern based on minimizing an objective function that is defined to be an average distance measure and such a pattern can be made adaptive to the motion statistics. Then, they suggest a regular checking point pattern which can be regarded as a sub-optimal solution. Simulation results show that motion estimation with the suggested pattern is both fast and robust.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Query previews in networked information systems (1997)

Dynamic queries have been shown to be an effective technique to browse information, and to find patterns and exceptions. Dynamic queries involve the interactive control by a user of visual query parameters that generate rapid (100 ms update), animated, and visual displays of database search results. The data of early implementations was stored in local memory to guarantee optimal speed. Problems arise when the data is very large and distributed over a network. To overcome the problems of slow networks and data volume we propose a two-phase approach to query formulation using query previews and query refinements [1]. Preview mechanisms have been used in the past [2] and we believe that their use will be a major component of successful networked information systems interfaces (e.g. [3]).
Effect of Electromagnetic Interference on Human Body Communication (2016)

In human body communication (HBC), the human body is used as a medium for transmitting data between devices as a replacement for wired and wireless technologies. The human body functions as an antenna in the low-frequency band used by HBC. Owing to this antenna function, electromagnetic waves radiating from electronic devices or wireless services cause electromagnetic interference (EMI) in HBC devices. This paper addresses such EMI in HBC devices. The interference signal caused by EMI was measured while the human subject, who was using an HBC device, was exposed to a general EMI environment at multiple sites. Using the interference model obtained from the measured interference signals, bit-error-rate degradation caused by the interference signal was simulated. The interference model presented in this paper can be effectively used to achieve reliable data communication in various HBC devices.
Biochemical basis of SOS-induced mutagenesis in <i>Escherichia coli</i> : Reconstitution of <i>in vitro</i> lesion bypass dependent on the UmuD′ <sub>2</sub> C mutagenic complex and RecA protein (1998)

Damage-induced SOS mutagenesis requiring the UmuD'C proteins occurs as part of the cells' global response to DNA damage. In vitro studies on the biochemical basis of SOS mutagenesis have been hampered by difficulties in obtaining biologically active UmuC protein, which, when overproduced, is insoluble in aqueous solution. We have circumvented this problem by purifying the UmuD'2C complex in soluble form and have used it to reconstitute an SOS lesion bypass system in vitro. Stimulated bypass of a site-directed model abasic lesion occurs in the presence of UmuD'2C, activated RecA protein (RecA*), beta-sliding clamp, gamma-clamp loading complex, single-stranded binding protein (SSB), and either DNA polymerases III or II. Synthesis in the presence of UmuD'2C is nonprocessive on damaged and undamaged DNA. No lesion bypass is observed when wild-type RecA is replaced with RecA1730, a mutant that is specifically defective for Umu-dependent mutagenesis. Perhaps the most noteworthy property of UmuD'2C resides in its ability to stimulate both nucleotide misincorporation and mismatch extension at aberrant and normal template sites. These observations provide a biochemical basis for the role of the Umu complex in SOS-targeted and SOS-untargeted mutagenesis.
An 81MHz, 1280 x 720pixels x 30frames/s MPEG-4 video/audio codec processor (2005)

A high-definition MPEG-4 CODEC processor capable of encoding 720p images (1280/spl times/720 pixels 30f/s) at 81MHz is presented. The CODEC is implemented with only 390k gates and an 80 kB SRAM. It is fabricated in a 0.13/spl mu/m CMOS process on a 5.6mm/spl times/5.6mm die.
Analysis of Proinflammatory and Anti-Inflammatory Cytokine Serum Concentrations in Patients With Multiple Sclerosis by Using a Multiplexed Immunoassay (2011)

We examined cytokines and other inflammatory markers in serum samples from 833 patients with multiple sclerosis and 117 healthy control subjects. A multiplexed immunoassay was used to assess the concentrations of 13 cytokines/inflammatory markers: interferon (IFN)-γ; interleukins (ILs)-1β, 2, 4, 5, 6, 8, 10, 12, and 13; tumor necrosis factor (TNF)-α; IL-2 receptor; and soluble CD40 ligand. Significant increases between patients and control subjects were found for IFN-γ (mean, 7.5 vs 0.4 pg/mL; P = .0002), IL-2 (mean 5.7 vs 1.0 pg/mL; P =.0002), IL-1β (mean, 23.0 vs 11.3 pg/mL; P ≤ .0001), TNF-α (mean, 4.1 vs 1.2 pg/mL; P = .01), IL-4 (mean, 1.4 vs 0.1 pg/mL; P ≤ .0001), IL-10 (mean, 16.8 vs 7.5 pg/mL; P = .03), and IL-13 (mean, 4.5 vs 0.8 pg/mL; P ≤ .0001). Profiling cytokines in multiple sclerosis may help to identify mechanisms involved in the pathogenesis of the disease, aid in monitoring the disease course and in evaluating responses to specific therapies, and, potentially, lead to new therapies directed at cytokines or their receptors.
Crystal structure of the DNA-recognition component of the bacterial virus Sf6 genome-packaging machine (2010)

In herpesviruses and many bacterial viruses, genome-packaging is a precisely mediated process fulfilled by a virally encoded molecular machine called terminase that consists of two protein components: A DNA-recognition component that defines the specificity for packaged DNA, and a catalytic component that provides energy for the packaging reaction by hydrolyzing ATP. The terminase docks onto the portal protein complex embedded in a single vertex of a preformed viral protein shell called procapsid, and pumps the viral DNA into the procapsid through a conduit formed by the portal. Here we report the 1.65 A resolution structure of the DNA-recognition component gp1 of the Shigella bacteriophage Sf6 genome-packaging machine. The structure reveals a ring-like octamer formed by interweaved protein monomers with a highly extended fold, embracing a tunnel through which DNA may be translocated. The N-terminal DNA-binding domains form the peripheral appendages surrounding the octamer. The central domain contributes to oligomerization through interactions of bundled helices. The C-terminal domain forms a barrel with parallel beta-strands. The structure reveals a common scheme for oligomerization of terminase DNA-recognition components, and provides insights into the role of gp1 in formation of the packaging-competent terminase complex and assembly of the terminase with the portal, in which ring-like protein oligomers stack together to form a continuous channel for viral DNA translocation.
[Latest progress in maxillofacial X-ray study and surgery in treatment of congenital and acquired deformities of viscera cranium in adults]. (2000)

The communication outlines the present-day potentialities of X-ray diagnosis and maxillofacial surgery in recognizing and treating congenital and acquired deformities of the visceral cranium.
A general Sub-Band Coding Scheme for 140 Mbit/s HDTV (1992)

A simple three dimensional sub-band splitting scheme for HDTV is presented. The aim is to compress the interlaced HDTV signal which comes from a bit rate of 880 Mbit/s, down to less than 140 Mbit/s. The scheme uses perfect reconstruction and QMF filter banks to split a sequence of frames in the temporal, horizontal and vertical direction. The temporal high bands contain the motion information. After the filtering, subsampling is used in the spatial as well as in the temporal domain. To the subsampled bands bits are allocated, depending on the activities in those bands. Each band is divided in three different activity zones for which different quantizers are used. The resulting values are VLC coded using arithmetic coding. With this technique, good distribution quality results have been achieved. >
Importance of the Dimer-Dimer Interface for Allosteric Signal Transduction and AMP Cooperativity of Pig Kidney Fructose-1,6-Bisphosphatase (1997)

The role of the 190's loop of fructose-1,6-bisphosphatase (Fru-1, 6-P2ase) in the allosteric regulation of Fru-1,6-P2ase has been investigated through kinetic studies on three mutant enzymes, Glu-192 --> Ala, Glu-192 --> Gln, and Asp-187 --> Ala. AMP is an allosteric inhibitor, which binds to the regulatory sites and induces the R- to T-state transition; for wild-type Fru-1,6-P2ase AMP inhibition is cooperative with a Hill coefficient of 2.0. The replacement of Asp-187, which forms an interaction across the C1:C2 monomer-monomer interface, with alanine did not change the catalytic efficiency, and it had no effect on the cooperativity of AMP inhibition; however, the apparent dissociation constant for AMP increased more than 4-fold as compared to the value for the wild-type enzyme. The replacement of Glu-192, which forms interactions across the C1:C4 dimer-dimer interface, with Ala and Gln lowered kcat from 21 s-1 for wild-type enzyme to 15 s-1 and 13 s-1, respectively, for the mutant enzymes, while their respective Km values were not changed. However, these replacements did have dramatic effects on AMP inhibition; first, cooperative AMP inhibition was lost; second, the AMP inhibition was biphasic, which can be interpreted as due to AMP binding to two classes of binding sites. The high affinity class of sites corresponds to the regulatory sites, while the low affinity class of sites may be the active sites. The results reported here, combined with the structural and kinetic results from the Lys-42 --> Ala enzyme, strongly suggest that the C1:C4 dimer-dimer interface, rather than the C1:C2 monomer-monomer interface, is critical for the propagation of the allosteric signal between the AMP sites on different subunits; in addition, cooperative AMP inhibition is essential for the enzyme to be fully inhibited by the binding of AMP to the allosteric site.
Crack Deflection Process for Hot‐Pressed Whisker‐Reinforced Ceramic Composites (1989)

A crack deflection model for two‐dimensional randomly arranged rods has been derived by making an appropriate modification to the model by Faber and Evans. This model predicts more effective toughening in certain directions than that for three‐dimensional randomly arranged rods. The theoretical predictions are compared with experimentally measured fracture toughness data of hot‐pressed whiskerreinforced ceramic‐matrix composites.
Erratum: Quantitative vectorial spin analysis in angle-resolved photoemission:<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:mi mathvariant="normal">Bi</mml:mi><mml:mo>∕</mml:mo><mml:mi mathvariant="normal">Ag</mml:mi><mml:mrow><mml:mo>(</mml:mo><mml:mn>111</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:mrow></mml:math>and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:mi mathvariant="normal">Pb</mml:mi><mml:mo>∕</mml:mo><mml:mi mathvariant="normal">Ag</mml:mi><mml:mrow><mml:mo>(</mml:mo><mml:mn>111</mml:mn><mml:mo>)</mml:mo></mml:mrow></mml:mrow></mml:math>[Phys. Rev. B<b>77</b>, 165431 (2008)] (2009)

The concept of vectorial spin analysis in spin and angle resolved photoemission is illustrated in this report. Two prototypical systems, Bi/Ag(111)(sqrt3 x sqrt3)R30 and Pb/Ag(111)(sqrt3 x sqrt3)R30, which show a large Rashba type spin-orbit splitting, were investigated by means of spin and angle resolved photoemission. The spin polarization vectors of individual bands are determined by a two-step fitting routine. First, the measured intensities are fitted with an appropriate number of suitable peaks to quantify the contributions of the individual bands, then the measured spin polarization curves are fitted by varying for each band the polarization direction and its magnitude. We confirm that the surface states experience a large spin splitting. Moreover, we find that all surface state bands are 100 percent spin polarized, and that for some states spin polarization vectors rotate out of the surface plane.
Selective stimulation of the D1 ATPase domain of <i>N</i>‐ethylmaleimide‐sensitive fusion protein (NSF) by soluble NSF attachment proteins (1998)

N-Ethylmaleimide-sensitive fusion protein (NSF) is required for most intracellular membrane fusion events. NSF is recruited to membranes by soluble NSF attachment proteins (SNAPs) and membrane-resident SNAP receptor (SNARE) proteins. The 20 S complex of NSF/SNAPs/SNAREs disassembles when NSF hydrolyses ATP, and this disassembly event is believed to be essential for membrane fusion. SNAPs stimulate NSF ATPase activity, but it is not known which of NSF's two ATPase domains (D1 or D2) is affected. Using recombinant mutant NSFs defective in ATP hydrolysis in one domain only, we found that SNAPs stimulate NSF ATPase activity by a selective action on the D1 domain, yet had no effect on the D2 domain. Since the D1 domain of NSF is implicated in 20 S complex disassembly, this supports the idea that SNAP stimulation of NSF ATPase activity is required for membrane fusion.
Demetalation chemistry: control of vanadium on fluid cracking catalyst (1990)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDemetalation chemistry: control of vanadium on fluid cracking catalystJin S. Yoo, Emmitt H. Burk Jr., John A. Karch, and Andrew P. VossCite this: Ind. Eng. Chem. Res. 1990, 29, 7, 1183–1189Publication Date (Print):July 1, 1990Publication History Published online1 May 2002Published inissue 1 July 1990https://pubs.acs.org/doi/10.1021/ie00103a016https://doi.org/10.1021/ie00103a016research-articleACS PublicationsRequest reuse permissionsArticle Views132Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Time-domain broadband phase-only full-waveform inversion with implicit shaping (2017)

We propose a new full-waveform inversion (FWI) method that approximates broadband tomographic inversion and has a reduced sensitivity to errors in the observed-data amplitude information. The method is based on fitting the observed-data phase spectrum while automatically shaping forward-modeled wave fields to the observed-data amplitude spectrum. This is achieved by using a phase-only objective function that allows broadband time-domain inversion of the observed-data phase information. We demonstrate our method’s reduced sensitivity to dynamic information under the traveltime approximation, and compare the new objective function to the normalized L2 FWI objective function in an experiment on synthetic data with a frequency-dependent attenuation. Presentation Date: Monday, September 25, 2017 Start Time: 3:30 PM Location: 361F Presentation Type: ORAL
Spawning kinetics in the gilthead sea‐bream, <i>Sparus aurata</i> L. after low doses of human chronic gonadotropin (1979)

This paper reports the temporal pattern of hormonally induced spawning in Sparus aurata females reared in captivity. A low dose of human chorionic gonadotropin (100–400 i.u. kg −1 body weight), administered to females with oocytes in the last stages of vitello genesis, induced a daily cycle of maturation, ovulation and spawning of fertilizable eggs. This cyclic pattern lasted for a period of 4–100 days. In fish injected with saline and most of the untreated fish, all vitellogenetic eggs underwent rapid atresia.
Cellular mechanisms of the clearance function of type C receptors of atrial natriuretic factor. (1990)

Clearance (C) receptors of atrial natriuretic factor (ANF) have an important role of removing ANF from the circulation. In the present study we investigated the cellular mechanisms of this function. 125I-ANF1-28 specifically bound to C-ANF receptors in cultured bovine vascular smooth muscle cells is internalized by a temperature-dependent process at a rate of 5% occupied receptors/min at 37 degrees C. Internalized 125I-ANF1-28 is rapidly metabolized and released to the medium as [125I]monoiodotyrosine, a process that is reversibly inhibited by NH4Cl (10 mM). Retroendocytosis of receptor-ligand complex is detectable when intracellular accumulation of the ligand is maximized by previous incubation with NH4Cl. In the presence of saturating concentrations of 125I-ANF1-28 when cells are warmed from 4 to 37 degrees C, there is first a decrease and then an almost complete replenishment of surface C-ANF receptors, a phenomenon that is not altered by protein synthesis inhibition with cycloheximide. In the absence of temperature transition, the density of surface C-ANF receptors remains constant, and by 2 h of incubation the amount of ANF hydrolyzed far exceeds the total amount of C-ANF receptors. The total population of surface C-ANF receptors is internalized and recycled every hour, and these processes are constitutive since they also occur in the absence of ligand. Trypsinization and solubilization experiments further indicate that C-ANF receptors are rapidly internalized and recycled to the cell membrane. The results demonstrate that receptor-ligand internalization, extensive receptor recycling, and lysosomal hydrolysis of ANF are the cellular mechanisms by which C-ANF receptors exert their clearance function.
Identification of β-Lactamase in Antibiotic-Resistant <i>Bacillus cereus</i> Spores (2007)

Beta-lactamase type I is reported for the first time to occur in the sporulated form in a penicillin-resistant Bacillus species. The enzyme was readily characterized from the B. cereus 5/B line (ATCC 13061) by mass spectrometry and two-dimensional gel electrophoresis.
Structural basis for histone H3 Lys 27 demethylation by UTX/KDM6A (2011)

Tri- and dimethylations of histone H3K9 (H3K9me3/2) and H3K27 (H3K27me3/2), both situated in the "A-R-Kme-S" sequence motif, mediate transcriptional repression of distinct genomic regions. H3K9me3/2 mainly governs constitutive heterochromatin formation, while H3K27me3/2 represses key developmental genes. The mechanisms by which histone-modifying enzymes selectively regulate the methylation states of H3K9 and H3K27 are poorly understood. Here we report the crystal structures of the catalytic fragment of UTX/KDM6A, an H3K27me3/2-specific demethylase, in the free and H3 peptide-bound forms. The catalytic jumonji domain binds H3 residues 25-33, recognizing H3R26, H3A29, and H3P30 in a sequence-specific manner, in addition to H3K27me3 in the catalytic pocket. A novel zinc-binding domain, conserved within the KDM6 family, binds residues 17-21 of H3. The zinc-binding domain changes its conformation upon H3 binding, and thereby recognizes the H3L20 side chain via a hydrophobic patch on its surface, which is inaccessible in the H3-free form. Mutational analyses showed that H3R17, H3L20, H3R26, H3A29, H3P30, and H3T32 are each important for demethylation. No other methyllysines in the histone tails have the same set of residues at the corresponding positions. Thus, we clarified how UTX discriminates H3K27me3/2 from the other methyllysines with distinct roles, including the near-cognate H3K9me3/2, in histones.
Multispectral microbolometers for the midinfrared (2010)

The spectral responsivity and the dynamic behavior of microbolometers with an integrated absorbing metamaterial are investigated. Wavelength tailoring and tuning in different microbolometers are achieved by varying the lateral extension of the absorber elements. Maximum sensitivity is tuned between 2.9 and 7.7 μm, with peak absorptions reaching up to 88%. The presence of a continuous metallic shielding layer affects heat conduction and leads to faster thermal response times.
C<sub>2</sub>-Bridged Titanocene−Ferrocenyl Complexes:  Synthesis, Reaction Chemistry, and Electrochemical Behavior (1998)

LiC⋮CFc (2; Fc = (η5-C5H4)(η5-C5H5)Fe) reacts with [Ti]Cl2 (1a) ([Ti] = (η5-C5H4SiMe3)2Ti) in a 2:1 molar ratio to produce the C2-bridged titanocene−ferrocenyl complex [Ti](C⋮CFc)2 (3). While treatment of heterotrinuclear 3 with Ni(CO)4 (4) affords the heterotetranuclear tweezer complex {[Ti](C⋮CFc)2}Ni(CO) (5), reaction of 3 with NiCl2 (6) yields FcC⋮CC⋮CFc (7) along with [Ti]Cl2 (1a) and Ni0 in a redox reaction. 7 can also be synthesized in the chemical oxidation of 3 with 2 equiv of AgPF6. The electrochemical behavior of compounds 3 and 5 is discussed. The X-ray structure analysis of complex 3 is reported.