gebhart/patrepeval huggingface dataset:
Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1. (1996)

A human cytochrome P-450 (P450) 1B1 cDNA was expressed in Saccharomyces cerevisiae and the microsomes containing P450 1B1 were used to examine the selectivity of this enzyme in the activation of a variety of environmental carcinogens and mutagens in Salmonella typhimurium TA1535/pSK1002 or NM2009 tester strains, using the SOS response as an end point of DNA damage. We also determined and compared these activities of P450 1B1 with those catalyzed by recombinant human P450s 1A1 and 1A2, which were purified from membranes of Escherichia coli. The carcinogenic chemicals tested included 27 polycyclic aromatic hydrocarbons and their dihydrodiol derivatives, 17 heterocyclic and aryl amines and aminoazo dyes, three mycotoxins, two nitroaromatic hydrocarbons, N-nitrosodimethylamine, vinyl carbamate, and acrylonitrile. Among the three P450 enzymes examined here, P450 lB1 was found to have the highest catalytic activities for the activation of 11,12-dihydroxy-11,12-dihydrodibenzo[a,l]pyrene, 1,2-dihydroxy-1,2-dihydro-5-methylchrysene, (+)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, 11,12-dihydroxy-11,12-dihydrobenzo[g]chrysene, 3,4-dihydroxy-3,4-dihydrobenzo[c]phenanthrene, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole, 2-aminoanthracene, 3-methoxy-4-aminoazobenzene, and 2-nitropyrene. P450 1B1 also catalyzed the activation of 2-amino-3,5-dimethylimidazo[4,5-f]quinoline, 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, 2-amino-3-methylimidazo[4,5-f]quinoline, 2-aminofluorene, 6-aminochrysene and its 1,2-dihydrodiol, (-)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene, 1,2-dihydroxy-1,2-dihydrochrysene, 1,2-dihydroxy-1,2-dihydro-5,6-dimethylchrysene, 2,3-dihydroxy-2,3-dihydrofluoranthene, 3,4-dihydroxy-3,4-dihydro-7,12-dimethylbenz[a]anthracene, and 6-nitrochrysene to appreciable extents. However, P450 1B1 did not produce genotoxic products from benzo[a]pyrene, trans- 3,4-dihydroxy-3,4-dihydrobenzo[a]anthracene, trans-8,9-dihydroxy-8,9-dihydrobenzo[a]anthracene, 7,12-dimethylbenz[a]anthracene and its cis-5,6-dihydrodiol, 5-methylchrysene, 11,12-dihydroxy-11,12-dihydro-3-methylcholanthrene, 1,2-dihydroxy-1,2-dihydro-6-methylchrysene, benzo[c]phenanthrene, 2-amino-6-methyldipyridol[1,2-a:3',2'-d]imidazole, 2-acetylaminofluorene, benzidine, 2-naphthylamine, aflatoxin B1, aflatoxin G1, sterigmatocystin, N-nitrosodimethylamine, vinyl carbamate, or acrylonitrile in this assay system. P450 1B1 is expressed constitutively in extrahepatic organs, including fetal tissue samples, and is highly inducible in various organs by 2,3,7,8-tetrachlorodibenzo-p-dioxin and related compounds in experimental animal models. Thus, activation of procarcinogens by P450 lB1 may contribute to human tumors of extrahepatic origin.
Universal utility interface for Plug-in Hybrid electric vehicles with vehicle-to-grid functionality (2010)

This paper proposes a novel topology for Electrical Vehicles and Plug-in Hybrid Electrical Vehicles with controllable power factor with 3-phase input and unity power factor with single phase input. The proposed on-board topology provides bidirectional power flow to/from the grid, low weight, low volume, and isolation. A control scheme is devised and a complete simulation of power control is performed over varying loads, varying input voltages, three phase or single phase input, and switching schemes. Simulation results are presented that demonstrate the ability to control power flow and synthesize the grid currents to be sinusoidal.
Thrombin activation of PAR-1 contributes to microvascular stasis in mouse models of sickle cell disease (2020)

Vaso-occlusive crisis (VOC) is the primary cause of morbidity and hospitalization in sickle cell disease (SCD); however, only 4 therapies (hydroxyurea, l-glutamine, crizanlizumab, and voxeletor) are currently approved in SCD. These agents limit the duration, severity, and frequency of crises. Activation of coagulation is a hallmark of SCD. Studies in animal models of SCD have shown that coagulation contributes to the chronic inflammation and end-organ damage associated with the disease; however, it is unknown whether coagulation directly contributes to the microvascular stasis that causes VOC. Herein, we demonstrate that inhibition of tissue factor (TF) and the downstream coagulation proteases factor Xa and thrombin significantly attenuates heme-induced microvascular stasis in mouse models of VOC. Pharmacologic inhibition of the principal thrombin receptor, protease activated receptor-1 (PAR-1), as well as deficiency of PAR-1 in all nonhematopoietic cells, also reduces stasis in sickle mice. PAR-1 deficiency was associated with reduced endothelial von Willebrand factor expression, which has been shown to mediate microvascular stasis. In addition, TF inhibition reduces lung vaso-occlusion in sickle mice mediated by arteriolar neutrophil-platelet microemboli. In sum, these results suggest that prophylactic anticoagulation might attenuate the incidence of VOC.
A DYNAMIC MODEL ON THE TRI-REFORMER FIXED-BED REACTOR TO PREPARE SYNTHESIS GAS REQUIRED TO PRODUCE METHANOL (2016)

The tri-reformer reactors play an important role in methanol production units, enhancing efficiency of synthesis gas preparation required to produce methanol. In the present work, using energy and mass balance and by exerting an appropriate kinetic, a proper dynamic and homogeneous model was proposed. The obtained equations were solved by means of MATLAB software. In certain circumstances, changes of various parameters versus length of the rector and time were examined. Eventually, effects of the major parameters, including inlet temperature and ratio of inlet material (CO 2 /CH 4 , O 2 /CH 4 and steam/CH 4 ), using the proposed model were studied. The results showed that increase of oxygen to methane ratio and steam to methane ratio, and also decrease of the temperature led to enhance hydrogen amount in product.
Reduced Oviposition by <I>Diaprepes abbreviatus</I> (Coleoptera: Curculionidae) and Growth Enhancement of Citrus by Surround Particle Film (2006)

Regularly applied sprays of a particle film, Surround WP, greatly enhanced the growth of citrus trees on a poorly drained Winder soil at Fort Pierce, FL. After 3 yr of applications every 3 or 4 wk, Surround-treated trees had at least 5 times the mass, 6 times the canopy volume, and approximately 4 times the cross-sectional area of the tree stems at the graft union compared with untreated trees. The larger Surround-treated trees attracted a higher number of adult weevil Diaprepes abbreviatus (L.) and to a lesser extent citrus root weevil, Pachnaeus litus (Germar), per tree, but there was an equivalent number of egg masses per tree compared with the control trees. The number of egg masses per female weevil oviposited on Surround-treated trees was significantly less than either the control trees or trees treated biannually with an entomopathogenic nematode, BioVector. The number of larvae per tree recovered from the roots of excavated trees was greater from trees treated with Surround once every 3 wk compared with control trees. The data suggest that Surround particle film greatly enhanced the growth of citrus trees grown in a poorly drained soil. The reduction in oviposition by D. abbreviatus was insufficient to significantly reduce the number of root weevil larvae per tree feeding on the roots. However, the more vigorous trees resulting from Surround applications may be more resistant or tolerant to root weevil feeding.
A Robust Stereo Matching Method for Low Texture Stereo Images (2009)

Computing disparity images for stereo pairs of low texture images is a challenging task because matching costs inside low texture areas of the stereo pairs are almost similar. This problem can not be solved straightforwardly by increasing the size of aggregation windows or by using global optimization methods, e.g. dynamic programming, because those approaches will smooth depth discontinued boundaries as well. Based on the assumption that disparities of pixels in homogeneous regions are similar, this paper proposes a new method that is able to robustly perform stereo matching for low texture stereo images. The proposed method utilizes the edge maps computed from the stereo pairs to guide the cost aggregation process in stereo matching. By using edge maps, the proposed method can achieve the effect of using different shapes and sizes of aggregation windows. Moreover, the computational complexity of the proposed method is independent from the window size, similar to the moving average aggregation method. Experimental results from both of an artificial and a real stereo image sequence demonstrate that the proposed method can produce a larger number of and a better accuracy of reliable disparities for low texture stereo images than the moving average method.
The contributions of central versus peripheral vision to scene gist recognition (2009)

Which region of the visual field is most useful for recognizing scene gist, central vision (the fovea and parafovea) based on its higher visual resolution and importance for object recognition, or the periphery, based on resolving lower spatial frequencies useful for scene gist recognition, and its large extent? Scenes were presented in two experimental conditions: a "Window," a circular region showing the central portion of a scene, and blocking peripheral information, or a "Scotoma," which blocks out the central portion of a scene and shows only the periphery. Results indicated the periphery was more useful than central vision for maximal performance (i.e., equal to seeing the entire image). Nevertheless, central vision was more efficient for scene gist recognition than the periphery on a per-pixel basis. A critical radius of 7.4 degrees was found where the Window and Scotoma performance curves crossed, producing equal performance. This value was compared to predicted critical radii from cortical magnification functions on the assumption that equal V1 activation would produce equal performance. However, these predictions were systematically smaller than the empirical critical radius, suggesting that the utility of central vision for gist recognition is less than predicted by V1 cortical magnification.
Association-based similarity testing and its applications (2003)

This paper proposes a new similarity measure between basket datasets based on associations. The new measure is calculated from support counts using a formula inspired by information entropy. Experiments on both real and synthetic datasets show the ef
SALT ACCLIMATION OF TRITICUM-AESTIVUM BY CHOLINE CHLORIDE - PLANT-GROWTH, MINERAL-CONTENT, AND CELL-PERMEABILITY (1993)

Seedlings of a salt sensitive line of Triticum aestivum were grown in Hoagland solution supplemented with 100 mM NaCl following a pretreatment with choline chloride (ChCl). Changes in growth, mineral content of roots and shoots, and passive permeability of the cell membrane were measured. Relative growth of ChCl pretreated plants after exposure to NaCl maximized at 0.5 mM ChCl reaching about control values. Mineral content (except Na+ and C1-) was lowered by NaCl treatment. Choline chloride pretreatment at all concentrations decreased cell membrane permeability for urea and methylurea with little effect of a subsequent NaCl treatment. The results suggested that ChCl acclimated the salt sensitive wheat cultivar (with 0.5 mM ChCl the most effective concentration). It is proposed that this acclimation involves two components: (a) modification of the membrane phospholipid composition and structure by the ChCl pretreatment, and (b) enabling of the cell metabolism to function near to normal under saline conditions after ChCl pretreatment.
A highly copper selective chromogenic calix[4]arene derivative (2010)

In this article, copper selective complexation behavior of 5,11,17,23-tetrakis[(N,N-dicyclohexylamino)methyl]-25,26,27,28-tetrahydroxycalix[4]arene (4) has been explored. Besides this, the binding ability of 4 toward different metal ions such as Cd2+, Co2+, Cu2+, Hg2+, Ni2+, Pb2+ and Zn2+ has been investigated by UV-visible and fluorescence spectroscopies. The chromoionophore 4 shows pronounced selectivity for Cu2+ even in the presence of other metal ions. The results reveal that Cu2+ forms 1 ∶ 1 host–guest complex with 4. The FT-IR spectroscopic method has also been applied for further confirmation of complex formation between 4 and Cu2+ and found to be satisfactory.
Effect of Immune Heterozygous Spleen Cell Transfer on Resistance to Mouse Hepatitis Virus Infection in Nude Mice (1981)

The role of cell mediated immune response to mouse hepatitis virus (MHV) infection in mice was studied by transferring spleen cells from immune heterozygous littermates (nu/+). A suppressive effect on viral growth was seen in infected nude (nu/nu) mice, whereas immune nu/+ serum transfer had no effect. The protective effect of immune nu/+ spleen cells was significantly reduced by treatment with anti-theta serum plus complement but not with anti-Ig serum. In infected nu/nu mice which received transfers of immune nu/+ cells, neutralizing antibody appeared although the titer was not high enough to protect nu/nu mice from fatal infection. Histopathologically, lymphocyte infiltration in hepatic lesions was evident in infected nu/nu mice with nu/+ cell transfer, while it was slight without nu/+ cell transfer.
An approach on the potential use of probiotics in the treatment of skin conditions: acne and atopic dermatitis (2018)

Acne and Atopic Dermatitis (AD) are chronic inflammatory skin conditions with severe impact on a patient's life. Current treatments are related to adverse effects and do not represent a definitive cure. The present paper reviews the alterations in skin microbiome, specifically in acne and AD, and aims in searching for potential treatments based on benefic microorganisms, called probiotics. The review was made through bibliographic search of the main databases (Science Direct, PubMed, Scielo, Medline) between September 2015 and June 2016. Acne lesions create an environment that facilitates the excess growth of Propionibacterium acnes (P. acnes). AD is related to an increase in the proportion of Staphylococcus aureus (S. aureus) during flare-ups. Some microorganisms have been shown to act not only in the prevention but also in the competition for pathogenic microorganisms and beneficially affect the inflammatory process present in these conditions. Despite the high variety of tested bacteria, Staphylococcus, Streptococcus, Lactococcus, Lactobacillus, and Enterococcus are the ones which showed the highest potential to control acne, and Vitreoscilla filiformis (V. filiformis), Staphylococcus epidermidis (S. epidermidis), and species of Lactobacillus and Bifidobacterium in the treatment of AD. Many of these studies were in vitro, and more detailed research should be performed in order to prove the real efficacy and safety of probiotics in these situations. An interesting alternative seems to be the use of Bacteriocin-like inhibitory substances produced by probiotics, responsible for their antimicrobial activity.
An Assessment of the Current LEO Debris Environment and the Need for Active Debris Removal (2010)

The anti-satellite test on the Fengun-1 C weather satellite in early 2007 and the collision between Iridium 33 and Cosmos 2251 in 2009 dramatically altered the landscape of the human-made orbital debris environment in the low Earth orbit (LEO). The two events generated approximately 5500 fragments large enough to be tracked by the U.S. Space Surveillance Network. Those fragments account for more than 60% increase to the debris population in LEO. However, even before the ASAT test, model analyses already indicated that the debris population (for those larger than 10 cm) in LEO had reached a point where the population would continue to increase, due to collisions among existing objects, even without any future launches. The conclusion implies that as satellites continue to be launched and unexpected breakup events continue to occur, commonly-adopted mitigation measures will not be able to stop the collision-driven population growth. To remediate the debris environment in LEO, active debris removal must be considered. This presentation will provide an updated assessment of the debris environment after the Iridium 33/Cosmos 2251 collision, an analysis of several future environment projections based on different scenarios, and a projection of collision activities in LEO in the near future. The need to use active debris removal to stabilize future debris environment will be demonstrated and the effectiveness of various active debris removal strategies will be quantified.
IPv6 Router Alert Option for MPLS Operations, Administration, and Maintenance (OAM) (2015)

RFC 4379 defines the MPLS Label Switched Path (LSP) Ping/Traceroute mechanism in which the Router Alert Option (RAO) MUST be set in the IP header of the MPLS Echo Request messages and may conditionally be set in the IP header of the MPLS Echo Reply messages depending on the Reply Mode used. While a generic "Router shall examine packet" Option Value is used for the IPv4 RAO, there is no generic RAO value defined for IPv6 that can be used. This document allocates a new, generic IPv6 RAO value that can be used by MPLS Operations, Administration, and Maintenance (OAM) tools, including the MPLS Echo Request and MPLS Echo Reply messages for MPLS in IPv6 environments. Consequently, it updates RFC 4379.
The Suramin Derivative NF449 Interacts with the 5-fold Vertex of the Enterovirus A71 Capsid to Prevent Virus Attachment to PSGL-1 and Heparan Sulfate (2015)

NF449, a sulfated compound derived from the antiparasitic drug suramin, was previously reported to inhibit infection by enterovirus A71 (EV-A71). In the current work, we found that NF449 inhibits virus attachment to target cells, and specifically blocks virus interaction with two identified receptors--the P-selectin ligand, PSGL-1, and heparan sulfate glycosaminoglycan--with no effect on virus binding to a third receptor, the scavenger receptor SCARB2. We also examined a number of commercially available suramin analogues, and newly synthesized derivatives of NF449; among these, NF110 and NM16, like NF449, inhibited virus attachment at submicromolar concentrations. PSGL-1 and heparan sulfate, but not SCARB2, are both sulfated molecules, and their interaction with EV-A71 is thought to involve positively charged capsid residues, including a conserved lysine at VP1-244, near the icosahedral 5-fold vertex. We found that mutation of VP1-244 resulted in resistance to NF449, suggesting that this residue is involved in NF449 interaction with the virus capsid. Consistent with this idea, NF449 and NF110 prevented virus interaction with monoclonal antibody MA28-7, which specifically recognizes an epitope overlapping VP1-244 at the 5-fold vertex. Based on these observations we propose that NF449 and related compounds compete with sulfated receptor molecules for a binding site at the 5-fold vertex of the EV-A71 capsid.
Identification of Chromatographic Peaks Using Lotus 1-2-3(R) (1988)

A multiple reference peak identification method using a Lotus 1-2-3® spreadsheet program is developed for off-line identification and editing of chromatographic peaks on a PC-XT/AT compatible computer. There is no apparent limit to the total number of reference peaks. Any peak, regardless of size, can be used as a reference peak as long as it is well-resolved and present in both the sample and reference chromatograms. The identification is made by matching normalized retention indices of peaks in the sample chromatogram with those of known components in a calibration chromatogram. The accuracy of peak assignment is typically better than 95%. The identification and editing of a chromatogram from a capillary gas chromatograph containing 200 peaks takes about 15 min. The software program is almost completely menu driven.
Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancer (2017)

Tumor-associated macrophages (TAMs), most of which display the immunosuppressive M2 phenotype, affect the tumor microenvironment and promote progression and metastasis in lung carcinoma. In this study, we analyzed clinical non-small cell lung cancer (NSCLC) samples and found that high densities of TAMs were associated with a poor prognosis in NSCLC patients. Moreover, the number of TAMs present correlated positively with expression of sex determining region Y (SRY)-related high mobility group box 9 (SOX9) in NSCLC tissues. TAMs secreted TGF-β, which increased SOX9 expression and promoted epithelial-to-mesenchymal transition (EMT) in lung cancer cells, thereby promoting tumor proliferation, migration, and invasion. SOX9 knockdown inhibited EMT, indicating that TGF-β-mediated EMT is SOX9-dependent. TGF-β induced SOX9 expression by upregulating the C-jun/SMAD3 pathway. These results indicate that TGF-β secreted by TAMs promotes SOX9 expression via the C-jun/SMAD3 pathway, thereby promoting tumor metastasis. The TGF-β/SOX9 axis may therefore be an effective target for the treatment of lung cancer.
Fish are like flies are like frogs: Conservation of dorsal‐ventral patterning mechanisms (1997)

Genetic analysis of Drosophila has shown that a morphogenetic gradient of the Transforming Growth Factor-beta family member dpp patterns the embryonic dorsal-ventral axis. Molecular and embryological evidence from Xenopus has strongly suggested a similar role for Bmp-4, the dpp homolog, in patterning the dorsal-ventral axis of chordates. A recent report has now identified mutations in two genes, dino and swirl, that disrupt dorsal-ventral patterning in the zebrafish Danio rerio. Characterization of these mutations parallels findings from Drosophila, thus establishing a genetic framework for the analysis of dorsal-ventral patterning in a vertebrate.
Bees aren't the only ones: swarming in Gram‐negative bacteria (1994)

Swarming is a form of active surface motility that is widespread among flagellated, Gram-negative bacteria. In the laboratory, growth of the bacteria on certain agar surfaces leads to induction of the differentiated swarmer-cell state. Swarmer cells are generally long and multinucleate, always hyperflagellated, and can move rapidly over the agar surface in a coordinated manner. Some swarm colonies exude large amounts of 'slime', which could be essential for promoting intimate cell-cell contacts during swarming. There is evidence that the differentiated swarmer-cell stage facilitates pathogenic associations with host tissue. Almost nothing is known about the molecular signalling mechanism of surface sensing. Increased viscosity appears to be sensed by several bacteria, but other environmental cues, specific to each bacterium, are also important. In organisms in which swarming motility has been studied in some detail, the chemotaxis system has been shown to play an important role. The recent discovery of swarming motility in two genetically well-characterized organisms--Escherichia coli and Salmonella typhimurium--should lead to rapid progress in understanding this process.