gebhart/patrepeval huggingface dataset:
Endoplasmic Reticulum Export of Glycosyltransferases Depends on Interaction of a Cytoplasmic Dibasic Motif with Sar1 (2003)

Membrane proteins exit the endoplasmic reticulum (ER) in COPII-transport vesicles. ER export is a selective process in which transport signals present in the cytoplasmic tail (CT) of cargo membrane proteins must be recognized by coatomer proteins for incorporation in COPII vesicles. Two classes of ER export signals have been described for type I membrane proteins, the diacidic and the dihydrophobic motifs. Both motifs participate in the Sar1-dependent binding of Sec23p-Sec24p complex to the CTs during early steps of cargo selection. However, information concerning the amino acids in the CTs that interact with Sar1 is lacking. Herein, we describe a third class of ER export motif, [RK](X)[RK], at the CT of Golgi resident glycosyltransferases that is required for these type II membrane proteins to exit the ER. The dibasic motif is located proximal to the transmembrane border, and experiments of cross-linking in microsomal membranes and of binding to immobilized peptides showed that it directly interacts with the COPII component Sar1. Sar1GTP-bound to immobilized peptides binds Sec23p. Collectively, the present data suggest that interaction of the dibasic motif with Sar1 participates in early steps of selection of Golgi resident glycosyltransferases for transport in COPII vesicles.
Syntheses of 7-Substituted-3-phenylisocoumarins and the Related Compounds and their Fluorescence Spectra (1969)

4-Nitrostilbene-2-carboxylic acid was treated with chlorine to obtain 7-nitro-trans-4-chloro-3-phenyl-3, 4-dihydroisocoumarin (7), mp 183484°C. 7-Nitro-3-phenylisocoumarin, mp 256257°C, obtained by dehydrochlorination of (7), could be reduced into 7-amino-3-phenylisocoumarin (5).Diazotization of (5) with subsequent coupling with β-naphthol and β-naphthyl-amine gave red-colored compounds, (9), mp 253.5254.5°C, and (10), mp 248249°C, respectively. 7-Chloro- (11), mp 181. 5, -182. 5°C, and 7-bromo-3-phenyli-socoumarins (12), mp 185. 5186°C, were synthesized from (5) by the Sandmeyer reaction. 7- (2H-Naphtho [1, 2-d] p-triazol-2-yl) -3-phenylisocoumarin (1), mp 309 310°C, was obtained by refluxing (10) with cupric sulfate in pyridine. By heating a mixture of (1), aqueous ammonia, and methyl cellosolve in the autoclave at 200220°C for 10 hrs, 7- (2H-naphtho [1, 2-d] -triazol-2-yl) -3-phenylisocarbostyril (2), mp 333.5334.5°C, was prepared.2- (3-Carboxy-4-styrilphenyl) -2H-naphtho [1, 2-d] -triazole and 2- (p-styrilphe-ny1) -2H-naphtho [1, 2-d] -triazole were also synthesized for comparison. Their infrared, ultraviolet absorption and fluorescence spectra and that of (1) and (2) were measured and discussed.
Intraarticular gene transfer of thrombospondin‐1 suppresses the disease progression of experimental osteoarthritis (2010)

In osteoarthritis, angiogenesis, which occurs in the osteochondral junction and synovium, may accelerate inflammation and contribute to the severity of the disease. We used anterior cruciate ligament-transection (ACLT) to investigate the therapeutic effect of an angiogenesis inhibitor, thrombospondin-1 (TSP-1), in a rat model of osteoarthritis. Osteoarthritis was induced in Wistar rats in the knee of one hind leg. After ACLT, AdTSP-1 (adenoviral vector encoding mouse TSP-1) was intraarticularly injected into the knee joints. Transgene expression, angiogenesis, and inflammatory responses in the knee joints were examined. They were also assessed morphologically, radiographically, and histologically for manifestations of disease. The levels of TSP-1 peaked on day 3 and were substantially maintained for at least 9 days after AdTSP-1 infection. Adenovirus-mediated gene expression was detected in the synovial membrane and chondrocytes. TSP-1 gene transfer induced transforming growth factor-β (TGF-β) production, but it reduced microvessel density, macrophage infiltration, and interleukin-1β (IL-1β) levels. Gross morphological and histopathological examinations revealed that rats treated with AdTSP-1 had less severe osteoarthritis than controls. In vivo adenovirus-mediated TSP-1 gene transfer significantly reduced microvessel density, inflammation, and suppressed the progression of osteoarthritis. This study provides potential applications of TSP-1 gene delivery for treating osteoarthritis.
Preparation and Properties of Pyrolytic Silicon Nitride (1966)

Silicon nitride has very attractive electrical, physical, and chemical properties which are of great interest for microelectronic devices. Until recently, most methods that have been reported in literature produce silicon nitride in the form of powder, needles, ribbons, or flakes. Recently, Sterling and Swann reported that continuous films of silicon nitride have been obtained. A new pyrolytic method for preparing pinhole‐free continuous silicon nitride film is described. Films have been successfully prepared in the temperature range of 750°–1100°C. Some physical and chemical properties of those films are summarized.
Palladium-Catalyzed Carbon-Carbon Bond Formation on a Solid Phase Utilizing Hypervalent Iodonium Salts (1998)

Abstract Palladium-catalyzed cross-coupling of hypervalent iodonium salts with polymer bound alkene, alkyne, and boronate was achieved under mild conditions.
Construction of a Yeast Strain Deleted for theTRP1 Promoter and Coding Region that Enhances the Efficiency of the Polymerase Chain Reaction-Disruption Method (1997)

The sequence of the genome of Saccharomyces cerevisiae was recently determined. As well as all the informations concerning the structure of the chromosomes the scientific community had to deal with the discovery of dozens of new open reading frames (ORFs) of unknown function. The study of these ORFs requires the development of simple procedures that can be used on a large scale. In the framework of a European Pilot Project we have described a new approach for deleting ORFs. This method is based on transformation with a polymerase chain reaction product but is limited by the use of a strain deleted for the auxotropic marker. We present here the construction of a new recipient strain that lacks the TRP1 region and that allows a high efficiency of gene deletion.
Computational Histological Staining and Destaining of Prostate Core Biopsy RGB Images with Generative Adversarial Neural Networks (2018)

Histopathology tissue samples are widely available in two states: paraffin-embedded unstained and non-paraffin-embedded stained whole slide RGB images (WSRI). Hematoxylin and eosin stain (H&E) is one of the principal stains in histology but suffers from several shortcomings related to tissue preparation, staining protocols, slowness and human error. We report two novel approaches for training machine learning models for the computational H&E staining and destaining of prostate core biopsy RGB images. The staining model uses a conditional generative adversarial network that learns hierarchical non-linear mappings between whole slide RGB image (WSRI) pairs of prostate core biopsy before and after H&E staining. The trained staining model can then generate computationally H&E-stained prostate core WSRIs using previously unseen non-stained biopsy images as input. The destaining model, by learning mappings between an H&E stained WSRI and a non-stained WSRI of the same biopsy, can computationally destain previously unseen H&E-stained images. Structural and anatomical details of prostate tissue and colors, shapes, geometries, locations of nuclei, stroma, vessels, glands and other cellular components were generated by both models with structural similarity indices of 0.68 (staining) and 0.84 (destaining). The proposed staining and destaining models can engender computational H&E staining and destaining of WSRI biopsies without additional equipment and devices.
Reducing phosphorus runoff and improving poultry production with alum (1999)

This is a review paper on the effects of aluminum sulfate (alum) on ammonia volatilization and P runoff from poultry litter. Initially, laboratory studies were conducted that showed P solubility could be reduced in poultry litter with Al, Ca, and Fe amendments, indicating that these amendments may reduce P runoff. These results were confirmed in small plot studies in which alum applications to litter were shown to decrease P concentrations in runoff by as much as 87%, while improving tall fescue yields. Leaf tissue analyses indicated that the yield improvements were due to increased N availability, which we hypothesized was due to reduced NH3 volatilization. This result was confirmed in laboratory studies that showed that alum was one of the most effective (and cost-effective) compounds for reducing NH3 volatilization. Field trials conducted at commercial broiler farms in conjunction with the Environmental Protection Agency showed that alum additions to poultry litter lowered litter pH, particularly during the first 3 to 4 wk of each growout, which resulted in less NH3 volatilization and lower atmospheric NH3. Ammonia volatilization rates were reduced by 97% for the first 4 wk of the growout. Broilers grown on alum-treated litter were heavier than the controls (1.73 vs 1.66 kg) and had lower mortality (3.9 vs 4.2%) and better feed efficiency (1.98 vs 2.04). Electricity and propane use were lower for alum-treated houses. As a result of these economic benefits to the integrator and grower, the benefit:cost ratio of alum addition was 1.96. Phosphorus concentrations in runoff from small watersheds were 75% lower from alum-treated litter than normal litter over a 3-yr period. Long-term small plot studies on alum use have shown that alum-treated litter results in lower soil test P levels than normal litter and does not increase Al availability in soils or uptake by plants.
First-in-human trial of the safety, pharmacokinetics and immunogenicity of a PEGylated anti-CD40L antibody fragment (CDP7657) in healthy individuals and patients with systemic lupus erythematosus (2015)

Single doses of CDP7657 showed predictable PK in healthy individuals and patients with SLE and were well tolerated, with no safety signals of concern. These findings support further investigation of CDP7657 as a therapy for SLE.
Oral use of interferon therapy in cervical human papillomavirus infection. (2000)

The efficacy and the optimal dose of systemic alpha-interferon treatment of genital human papillomavirus infection is now under discussion because of high cost, low compliance and systemic toxicity of this drug. Recent studies seem to suggest that natural alpha-interferon may also have antiviral efficacy in humans after oral administration of low doses of the drug. For this reason, the efficacy and side-effects of an oral preparation of natural alpha-Interferon (OROFERONE-IFI) have been evaluated in a randomized, placebo controlled trial. We report here the results of this preliminary study. Low doses (150U tid for 8 weeks) of the drug were administered to 12 patients with mild cervical dysplasia and human papillomavirus infection. A significantly higher proportion of Interferon treated subjects showed a colposcopic and histological regression of the lesion after months of treatment in comparison with 9 placebo treated patients (75% vs 30%) without major side effects. These results seem to justify wider evaluations of this approach to Interferon treatment characterized by lower costs and fewer side effects in comparison with traditional systemic high dose treatment of alpha Interferon.
Non-contact Ultrasonic Inspection Technology of Fillet Weldments (2005)

The non-destructive Inspection of the fillet weldment has difficulties due to its geometrical complexity and uneasy access. The surface shear horizontal wave (SH-wave), however, has been successfully applied to the detection of cracks on the surface and sub-surface of the filet weldment heel part. The conventional ultrasonic inspection using the surface SH-wave is usually a contact method using piezoelectric transducer. Thus, it is not suitable for a field application because the reliability and repeatability of inspection are significantly affected by test conditions such as couplant, contact pressure and pre-process. In order to overcome this problem, a non-contact SH-wave inspection method using EMAT is propose. The experimental results with this non-contact method are compared with those with a conventional ultrasonic method in fillet weldment with slit type defects. It is shown that the non-contact inspection technique requires simple procedure and less time in the fillet weldment inspection.
ADDRESS TO THE SOCIETY1 (1932)

ADDRESS TO THE SOCIETY Get access R. S. PEARSON R. S. PEARSON Search for other works by this author on: Oxford Academic Google Scholar Forestry: An International Journal of Forest Research, Volume 6, Issue 1, 1932, Pages 1–4, https://doi.org/10.1093/oxfordjournals.forestry.a063256 Published: 01 April 1932
Partial error masking to reduce soft error failure rate in logic circuits (2004)

A new methodology for designing logic circuits with partial error masking is described. The key idea is to exploit the asymmetric soft error susceptibility of nodes in a logic circuit by targeting the error masking capability towards the nodes with the highest soft error susceptibility to achieve cost-effective tradeoffs between overhead and reduction in the soft error failure rate. Such techniques can be used in cost-sensitive high volume mainstream applications to satisfy soft error failure rate requirements at minimum cost. Two reduction heuristics, cluster sharing reduction and dominant value reduction, are used to reduce the soft error failure rate significantly with a fraction of the overhead required for conventional TMR.
Heme Protein Assisted Dispersion of Gold Nanoparticle Multilayers on Chips: From Stabilization to High-Density Double-Stranded DNAs Fabricated in Situ for Protein/DNA Binding (2009)

Heme proteins in general are shown to be an effective linking agent in stabilizing gold nanoparticles (AuNPs) and thus facilitate the fabrication of three-dimensional (3D) AuNP multilayers on a chip, resulting in a higher coating density than that on polymer linker anchored surfaces for analytical applications. With the use of electron spectroscopy for chemical analysis (ESCA) measurements, a lower oxidation state of Au(0) and dramatic changes among multiple chemical states of N1s are detected upon coating AuNPs with heme proteins but not detected upon coating AuNPs with non-heme proteins. Thus, we propose that the stabilization power arises from π-conjugation between AuNPs and the heme group. We also propose that such conjugation must be facilitated by the exposure of the heme group through a conformational change of the protein as well as interactions of other functional groups with AuNPs to bring the heme moiety to a close face-to-face distance with the AuNPs. A high-density double-stranded DNA (dsDNA) composed of a sequence of estrogen response element (ERE) is then fabricated on heme protein anchored chips. An in situ hybridization and tracking method is developed based on hybridization-induced fluorescence restoration associated with AuNPs and assists in the subsequent detection of DNA/protein binding on the same chip. The AuNP ERE chips are shown to have high sensitivity and specificity for quantitative detection of ERE binding with its two transcription factor isoforms, estrogen receptors α and β (ERα and ERβ), in cell lysates with reduced reagents and reaction time.
A formal framework to elicit roles with business meaning in RBAC systems (2009)

The role-based access control (RBAC) model has proven to be cost effective to reduce the complexity and costs of access permission management. To maximize the advantages offered by RBAC, the role engineering discipline has been introduced. A viable approach is to explore current applications and systems to find de facto roles embedded in existing user permissions, leading to what is usually referred to as role mining. However, a key problem that has not yet been adequately addressed by existing role mining approaches is how to propose roles that have business meaning. In order to do this, we provide a new formal framework that also enjoys practical relevance. In particular, the proposed framework leverages business information - such as business processes and organization structure - to implement role mining algorithms. Our key observation is that a role is likely to be meaningful from a business perspective when it involves activities within the same business process or organizational units within the same branch. To measure the spreading of a role among business processes or organization structure, we resort to centrality indices. Such indices are used in our cost-driven approach during the role mining process. Finally, we illustrate the application of the framework through a few examples.
Un algorithme de programmation quadratique en variables binaires (1969)

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Expression of free fatty acid receptor GPR40 in the neurogenic niche of adult monkey hippocampus (2007)

Polyunsaturated free fatty acids (PUFAs) are known to play critical roles for the development, maintenance, and function of the brain. Recently, we reported that G-protein coupled receptor 40 (GPR40), one type of PUFA receptors, is expressed throughout the adult primate central nervous system including the hippocampus. This opens a possibility that PUFA might act as extracellular signaling molecules at the GPR40 receptor to regulate neuronal function. Here we studied protein expression of GPR40 in the neurogenic niche of the adult monkey hippocampus under normal and postischemic conditions. Confocal laser microscope analysis of immunostained sections revealed GPR40 immunoreactivity in neural progenitors, immature neurons, astrocytes and endothelial cells of the subgranular zone (SGZ) of the dentate gyrus (DG); a well-known neurogenic niche within the adult brain. Immunoblotting analysis showed that the GPR40 protein increased significantly in the second week after global cerebral ischemia as compared with the control. This was compatible with the postischemic increment of GPR40-positive cells in the SGZ as detected by immunofluorescence imaging. Taken together with our previous findings of the SGZ progenitor cell upregulation after ischemia, the present data suggest that PUFA such as docosahexaenoic acid may act via GPR40 to regulate adult hippocampal neurogenesis in primates.
Reocclusion of recanalized arteries during intra-arterial thrombolysis for acute ischemic stroke. (2004)

Reocclusion occurs relatively frequently during intra-arterial thrombolysis for ischemic stroke and seems to be associated with poor clinical outcomes.
Fundamental Concepts of Synchronous Machine Reactances (1937)

This educational paper presents fundamental concepts of synchronous machine reactances in a form suitable for college seniors and recent graduates. Part I builds up the ``physical concepts'' of flux linkage and inductance, part II applies the concepts to synchronous machines, developing a physical picture of machine reactances. Part III starts with the fundamental circuit differential equations of a machine and obtains expressions for the reactances in terms of self- and mutual inductances, ending with the machine equations in the direct, quadrature, and zero axes, which give a physical picture of the machine ``viewed'' from these axes.
Design of Time and Frequency Domain Pilots for Generalized Multicarrier Systems (2007)

By the generalized multi-carrier (GMC) principle a unified framework to describe various multi-carrier as well as single carrier approaches is established. In this paper the GMC principle is extended to pilot design and channel estimation, so that a unified description of pilot aided channel estimation (PACE) by interpolation in time and frequency is established. This applies to frequency domain pilots which are embedded in the GMC signal, as well as pilots sequences time multiplexed with data-bearing GMC blocks. A comparative performance evaluation of the two different GMC variants OFDM and single carrier with various pilot allocation schemes is carried out, also taking into account practical constraints such as the peak to average power ratio (PAPR) and required power amplifier back-off. It is found that a serial modem with time or frequency multiplexed pilots could be designed with a power amplifier with about 2 dB lower maximum power rating than that of a corresponding OFDM modem.
Interferometric alignment of the X-SAR antenna system on the space shuttle radar topography mission (2002)

The on-orbit alignment of the antenna beams of both the X-band and C-band radar systems during operations of the shuttle radar topography mission/X-band synthetic aperture radar (SRTM/X-SAR) was a key requirement for achieving best interferometric performance. In this paper, we consider the X-SAR antenna beam alignment in azimuth. For a single-pass cross-track SAR interferometer, we establish the relation between yaw and pitch misalignment of the antenna beams and the resulting relative shift of the Doppler frequency bands. This relation is used to provide solutions for the mechanical adjustments of the outboard antenna and electronic beam steering to correct for azimuth misalignment. Furthermore, the effects of the X-SAR effective outboard antenna pattern on the azimuth beam alignment are analyzed. As a result, a so-called "relaxing" factor is derived, which increases the limit for the difference in antenna azimuth angle with respect to the requirement on spectral overlap, and hence spatial interferogram resolution. However, we also show that the alignment requirement is driven by the constraint on decreasing the azimuth ambiguity-to-signal ratio (AASR) for the effective outboard antenna pattern to reduce the resulting additional height error. The strategy for misalignment determination and correction is presented, and results of the analysis of the in-flight X-SAR antenna beam alignment are discussed.
Voltage-gated Mobility of the Ca2+ Channel Cytoplasmic Tails and Its Regulatory Role (2003)

Transient increase in intracellular free Ca(2+) concentration generated by the voltage-gated Ca(v)1.2 channels acts as an important intracellular signal. By using fluorescence resonance energy transfer combined with patch clamp in living cells, we present evidence for voltage-gated mobility of the cytoplasmic tails of the Ca(v)1.2 channel and for its regulatory role in intracellular signaling. Anchoring of the C-terminal tail to the plasma membrane caused an inhibition of its state-dependent mobility, channel inactivation, and CREB-dependent transcription. Release of the tail restored these functions suggesting a direct role for voltage-gated mobility of the C-terminal tail in Ca(2+) signaling.
Performance of television ghost cancelling systems under field test conditions (1992)

A field test measurement program evaluating five ghost cancelling systems for the NTSC television service was conducted in the Washington, DC, area in the fall of 1991. Data relating to the performance of the ghost cancelers under different multipath and ghosting conditions experienced in the field are presented and the test program is described. Subjective assessment of relative and absolute performance of the ghost cancelling systems indicated that one system exhibited consistently superior performance relative to the other four systems.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>