gebhart/patrepeval huggingface dataset:
Triboelectric Nanogenerator for Harvesting Wind Energy and as Self-Powered Wind Vector Sensor System (2013)

We report a triboelectric nanogenerator (TENG) that plays dual roles as a sustainable power source by harvesting wind energy and as a self-powered wind vector sensor system for wind speed and direction detection. By utilizing the wind-induced resonance vibration of a fluorinated ethylene-propylene film between two aluminum foils, the integrated TENGs with dimensions of 2.5 cm × 2.5 cm × 22 cm deliver an output voltage up to 100 V, an output current of 1.6 μA, and a corresponding output power of 0.16 mW under an external load of 100 MΩ, which can be used to directly light up tens of commercial light-emitting diodes. Furthermore, a self-powered wind vector sensor system has been developed based on the rationally designed TENGs, which is capable of detecting the wind direction and speed with a sensitivity of 0.09 μA/(m/s). This work greatly expands the applicability of TENGs as power sources for self-sustained electronics and also self-powered sensor systems for ambient wind detection.
A cohort pilot study on therapeutic vaccination of advanced melanoma patients with dendritic cells loaded with multiple peptides or electroporated with mRNA (2006)

18011 Background: Therapeutic vaccination of melanoma patients with tumor specific antigenic peptides has been associated with increased frequency of anti-vaccine CTL precursor frequencies in peripheral blood and objective tumor regression in a low percentage of patients. Methods: We investigated the safety and activity of two autologous dendritic cell-based vaccines in 2 sequential cohorts of advanced melanoma patients. Patients first underwent a leucapheresis. Following enrichment in a semi-closed culture system using Cell Factories (Nunc), adherent cells were cultured in RPMI 1640 medium supplemented with 1% heat-inactivated autologous plasma, 500U/ml IL-4 and 1000 U/ml GM-CSF for 6 days and cryo-preserved. Upon thawing, DC were pulsed with 8 peptides or electroporated with synthetic mRNA encoding 7 antigens. Dendritic cell vaccines were administered by intradermal and subcutaneous biweekly injections for a total of 6 times (= cycle 1). Thereafter vaccination was repeated every 6w until clinical deterioration or patient refusal. Frequencies of blood anti-vaccine CTL were estimated by in vitro restimulation in limiting dilution conditions followed by detection with tetramer assay. Results: Fifteen patients were recruited (9M/6F; median age was 49 y, range 28–81). Seven HLA-A2 patients were treated with peptide pulsed DC (cohort A) and 7 patients (4 HLA-A2 and 3 HLA-A1) with mRNA electroporated DC (cohort B). One patient was sequentially treated with both vaccines. Five patients in cohort A and 3 patients in cohort B completed the first treatment cycle of 6 bi-weekly vaccinations (cycle 1 is ongoing in 3 patients of cohort B). Toxicity was limited to grade 1 or 2 local skin reactions at the vaccine administration sites in all patients. In cohort B one patient qualified for stable disease and 2 other patients with progressive disease displayed regression of individual metastases. A >10-fold increase in CTL precursor frequency was observed in 2 out of 3 patients of cohort A that have been analyzed at present. Conclusions: Vaccination of melanoma patients with peptide pulsed or mRNA electroporated autologous DC vaccines was found to be feasible and safe. Updated results will be presented at the meeting. [Table: see text]
Competitive Inhibition of <i>hsp70</i> Gene Expression Causes Thermosensitivity (1988)

A novel method has been developed for modulating the expression of an endogenous chromosomal gene in a higher eukaryote, by competitive inhibition at the level of gene transcription. The gene studied was the hsp70 gene, which encodes a 72-kilodalton (kD) heat shock protein that is synthesized after thermal stress. The 5' control region of the hsp70 gene was inserted on a plasmid containing the eukaryotic gene for dihydrofolate reductase. The hybrid plasmid was then introduced into a Chinese hamster ovary cell line and elevated in copy number approximately 20,000-fold by selection of cells with methotrexate. Heat-inducible expression from the intact hsp70 gene was reduced by at least 90% in the modified cells when compared with the induction in control cells, and the modified cells also displayed elevated thermosensitivity. The change in heat shock protein synthesis is presumably caused by competition among the increased number of binding sites for the heat-shock transcription factor, leading to altered expression from the native heat shock gene. These results support a role for heat shock protein in the recovery of mammalian cells from acute thermal stress.
Vision Based Driver Assistance for Near Range Obstacle Sensing under Unstructured Traffic Environment (2019)

Lack of attention while navigating in unstructured traffic causes mishaps as road obstacles such as vehicles, jaywalkers do not follow road rules and their pattern of movement are highly unpredictable. This research work aims at developing a system that can be deployable on present vehicle for early detection and warning of road anomalies, so that evasive actions can be taken for safe navigation. We have developed near range surface obstacle sensing system using vision sensor, which can alert the driver, if any obstacle is entering the drive region or in the drive region within 15 meters. To improve real time performance of deep learning model, proposed methodology first predicts the drive region on road using monocular camera based on vanishing point (VP). YOLO (You Look At Once) darknet v3 detector is integrated with tracking algorithm SORT (Deep simple online and real time tracking) and multilevel perceptron neural network does early predication to alert the driver based on spatial information of detected obstacle. A visual alert will display the type of obstacle entering or within the drive region. The experiment result depict YOLO-SORT-MLP can ensure early detection and tracking of obstacle at real time at different time of day and weather conditions at 0.91 F1 score. The proposed methodology is been deployed on the test vehicle and tested on unstructured traffic environments.
A Cell-Based Small Molecule Screening Method for Identifying Inhibitors of Epithelial-Mesenchymal Transition in Carcinoma (2012)

Epithelial Mesenchymal Transition (EMT) is a crucial mechanism for carcinoma progression, as it provides routes for in situ carcinoma cells to dissociate and become motile, leading to localized invasion and metastatic spread. Targeting EMT therefore represents an important therapeutic strategy for cancer treatment. The discovery of oncogene addiction in sustaining tumor growth has led to the rapid development of targeted therapeutics. Whilst initially optimized as anti-proliferative agents, it is likely that some of these compounds may inhibit EMT initiation or sustenance, since EMT is also modulated by similar signaling pathways that these compounds were designed to target. We have developed a novel screening assay that can lead to the identification of compounds that can inhibit EMT initiated by growth factor signaling. This assay is designed as a high-content screening assay where both cell growth and cell migration can be analyzed simultaneously via time-course imaging in multi-well plates. Using this assay, we have validated several compounds as viable EMT inhibitors. In particular, we have identified compounds targeting ALK5, MEK, and SRC as potent inhibitors that can interfere with EGF, HGF, and IGF-1 induced EMT signaling. Overall, this EMT screening method provides a foundation for improving the therapeutic value of recently developed compounds in advanced stage carcinoma.
Bone morphogenetic protein‐7 enhances dendritic growth and receptivity to innervation in cultured hippocampal neurons (2000)

Members of the bone morphogenetic protein (BMP) family of growth factors are present in the central nervous system during development and throughout life. They are known to play an important regulatory role in cell differentiation, but their function in postmitotic telencephalic neurons has not been investigated. To address this question, we examined cultured hippocampal neurons following treatment with bone morphogenetic protein-7 (BMP-7, also referred to as osteogenic protein-1). When added at the time of plating, BMP-7 markedly stimulated the rate of dendritic development. Within 1 day, the dendritic length of BMP-7-treated neurons was more than twice that of controls. By three days the dendritic arbors of BMP-7-treated neurons had attained a level of branching similar to that of 2-week-old neurons cultured under standard conditions. Several findings indicate that BMP-7 selectively enhances dendritic development. While dendritic length was significantly increased in BMP-7-treated neurons, the length of the axon was not. In addition, the mRNA encoding the dendritic protein MAP2 was significantly increased by BMP-7 treatment, but the mRNA for tubulin was not. Finally, BMP-7 did not enhance cell survival. Because dendritic maturation is a rate-limiting step in synapse formation in hippocampal cultures, we examined whether BMP-7 accelerated the rate at which neurons became receptive to innervation. Using two separate experimental paradigms, we found that the rate of synapse formation (assessed by counting synapsin I-positive presynaptic vesicle clusters) was increased significantly in neurons that had been exposed previously to BMP-7. Because BMP-7 and related BMPs are expressed in the hippocampus in situ, these factors may play a role in regulating dendritic branching and synapse formation in both development and plasticity.
Functional characterization of the <i>Aspergillus fumigatus</i> CRZ1 homologue, CrzA (2008)

The protein phosphatase calcineurin is an important mediator connecting calcium-dependent signalling to various cellular responses in multiple organisms. In fungi calcineurin acts largely through regulating Crz1p-like transcription factors. Here we characterize an Aspergillus fumigatus CRZ1 homologue, CrzA and demonstrate its mediation of cellular tolerance to increased concentrations of calcium and manganese. In addition to acute sensitivity to these ions, and decreased conidiation, the crzA null mutant suffers altered expression of calcium transporter mRNAs under high concentrations of calcium, and loss of virulence when compared with the corresponding complemented and wild-type strains. We use multiple expression analyses to probe the transcriptional basis of A. fumigatus calcium tolerance identifying several genes having calA and/or crzA dependent mRNA accumulation patterns. We also demonstrate that contrary to previous findings, the gene encoding the Aspergillus nidulans calcineurin subunit homologue, cnaA, is not essential and that the cnaA deletion mutant shares the morphological phenotypes observed in the corresponding A. fumigatus mutant, DeltacalA. Exploiting the A. nidulans model system, we have linked calcineurin activity with asexual developmental induction, finding that CrzA supports appropriate developmental induction in a calcineurin and brlA-dependent manner in both species.
Restoration of responsiveness to phorbol ester by reconstitution of a functional Na/K/Cl cotransporter in cotransporter-deficient BALB/c 3T3 cells (1996)

Previous studies in this laboratory have implicated the membrane transport protein Na/K/Cl cotransporter (NKCC1) as an important component of the signaling pathways activated by phorbol esters in BALB/c 3T3 cells. The NKCC1 protein functions as a Na/K/Cl cotransporter in BALB/c 3T3 cells and many other cell types. Loss of NKCC1 function has been associated with loss of mitogenic responsiveness to phorbol ester. Here we report that expression of a cloned NKCC1 cDNA fused to a tetracycline-regulated promoter in BALB/c 3T3 cells deficient in Na/K/Cl cotransport activity (clone E12a cells) restored cotransport function. Compared with parental cotransport-deficient cells, transfected clones expressing the exogenous NKCC1 gene responded like typical BALB/c 3T3 cells to 12-O-tetradecanoylphorbol-13-acetate: loop diuretic-sensitive 86Rb+ flux was inhibited, cell volume was decreased, and cell growth was stimulated. These results support our previous conclusion that the loss of responsiveness of E12a cells to phorbol ester is caused by mutation of the endogenous NKCC1 gene.
Pressure Sensitive Sensors Based on Carbon Nanotubes, Graphene, and Its Composites (2018)

Carbon nanotubes (CNTs) and graphene have attracted a great deal of interest due to their outstanding mechanical, optical, electrical, and structural properties. Most of the scientists and researchers have investigated the optical and electrical properties of these materials. However, due to unique electromechanical properties of these materials, it is required to explore the piezoresistive properties of bulk nanostructured CNTs, graphene, and CNT-graphene composites. We investigated and compared the sensitivities and piezoresistive properties of sandwich-type pure CNT, pure graphene, and CNT-graphene composite pressure sensors. For all the samples, increase in pressure from 0 to 0.183 kNm −2 results in a decrease in the impedance and direct current (DC) resistance. Sensitivity and percentage decrease in resistance and impedance of CNT-graphene composite were lower than pure CNT while being higher than pure graphene based sample. Moreover, under the same external applied pressure, the sensitivity and percentage decrease in impedance for pure CNT, pure graphene, and CNT-graphene composite were smaller than the corresponding sensitivity and percentage decrease in resistance. The achieved experimental results of the composite sample were compared with simulated results which exhibit reasonable agreement with each other. The deviations of simulated resistance-pressure and impedance-pressure curves from experimental graphs were 0.029% and 0.105%, respectively.
Fidaxomicin Inhibits Spore Production in Clostridium difficile (2012)

Fidaxomicin (FDX) is a novel antimicrobial agent with narrow-spectrum and potent bactericidal activity against Clostridium difficile. In recent clinical trials, FDX was superior to vancomycin in preventing recurrences of C. difficile infection. A possible mechanism of reducing recurrence may be through an inhibitory effect on sporulation. The effect of FDX and its major metabolite, OP-1118, on C. difficile growth and sporulation kinetics was compared with that of vancomycin, metronidazole, and rifaximin. Drugs at subminimum inhibitory concentrations (sub-MICs) were added to cells at an early stationary phase of growth; this was followed by collection of cells at various intervals for quantitation of total viable cell and heat-resistant spore counts on taurocholate-containing media. The effect of the drugs at 2-2.5× MIC on the expression of sporulation genes in C. difficile was also compared using quantitative reverse-transcriptase polymerase chain reaction. Both FDX and OP-1118 (1/4× MIC) inhibited sporulation when added to early-stationary-phase cells in C. difficile strains, including the epidemic NAP1/BI/027 strain. In contrast, vancomycin, metronidazole, and rifaximin (at similar sub-MICs) did not inhibit sporulation. The number of spores following treatment with comparator drugs increased to the same level as the no-drug control treatment. Expression of mother cell-specific (spoIIID) and forespore-specific (spoIIR) sporulation genes also was inhibited by FDX and OP-1118 but not significantly by vancomycin. Both FDX and OP-1118 (unlike vancomycin, rifaximin, and metronidazole) effectively inhibited sporulation by C. difficile. The inhibitory effect of FDX on C. difficile sporulation may contribute to its superior performance in sustaining clinical response and reducing recurrences and may also be beneficial in decreasing shedding and transmission of this pathogen.
Generating hypermedia from specifications by sketching multimedia templates (1996)

This paper presents PageJokey, an object-oriented system for incrementally sketching multimedia templates, The goal is to provide designers with experimental feedback about the ultimate document, beginning from the earliest stages of design. Designers can use existing multimedia objects to create prototype templates, and incrementally generate and test draft versions of the final product using both bottom-up and top=down design approaches, PageJokey supports modular development, using the concepts of' reuse, abstraction and genericity for generating hypermedia documents.
GPS-based geographic addressing, routing, and resource discovery (1999)

article Free AccessGPS-based geographic addressing, routing, and resource discovery Authors: Tomasz Imieliński Rutgers Univ., Piscataway, NJ Rutgers Univ., Piscataway, NJView Profile , Julio C. Navas Rutgers Univ., Piscataway, NJ Rutgers Univ., Piscataway, NJView Profile Authors Info & Claims Communications of the ACMVolume 42Issue 4April 1999 pp 86–92https://doi.org/10.1145/299157.299176Published:01 April 1999Publication History 116citation2,786DownloadsMetricsTotal Citations116Total Downloads2,786Last 12 Months84Last 6 weeks18 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteView all FormatsPDF
Peroxo(tetraphenylporphinato)manganese(III) and chloro(tetraphenylporphinato)manganese(II) anions. Synthesis, crystal structures, and electronic structures. (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPeroxo(tetraphenylporphinato)manganese(III) and chloro(tetraphenylporphinato)manganese(II) anions. Synthesis, crystal structures, and electronic structures.Reuel B. VanAtta, Charles E. Strouse, Louise Karle Hanson, and Joan Selverstone ValentineCite this: J. Am. Chem. Soc. 1987, 109, 5, 1425–1434Publication Date (Print):March 1, 1987Publication History Published online1 May 2002Published inissue 1 March 1987https://pubs.acs.org/doi/10.1021/ja00239a024https://doi.org/10.1021/ja00239a024research-articleACS PublicationsRequest reuse permissionsArticle Views975Altmetric-Citations144LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
The Radon Transform (1980)

The first edition of this book has been out of print for some time and I have decided to follow the publisher's kind suggestion to prepare a new edition. Many examples with explicit inversion formulas and range theo- rems have been added, and the group-theoretic viewpoint emphasized. For example, the integral geometric viewpoint of the Poisson integral for the disk leads to interesting analogies with the X-ray transform in Euclidean 3-space. To preserve the introductory flavor of the book the short and self-contained Chapter Von Schwartz' distributions has been added. Here 5 provides proofs of the needed results about the Riesz potentials while 3-4 develop the tools from Fourier analysis following closely the account in Hormander's books (1963] and [1983]. There is some overlap with my books (1984] and [1994b] which however rely heavily on Lie group theory. The present book is much more elementary. I am indebted to Sine Jensen for a critical reading of parts of the manuscript and to Hilgert and Schlichtkrull for concrete contributions men- tioned at specific places in the text. Finally I thank Jan Wetzel and Bonnie Friedman for their patient and skillful preparation of the manuscript.
Polar Head Group Decarboxylation and Methylation of Phospholipids: An Alternate Route for Phosphatidylcholine Formation in Cultured Neuronal Cells (1985)

Decarboxylation of phosphatidylserine (SPG) and methionine-dependent, stepwise methylation of phosphatidylethanolamine (EPG) to form phosphatidylcholine (CPG) were examined in monolayer cultures of rat cerebral cells. Ethanolamine, monomethylaminoethanol, or dimethylaminoethanol nitrogenous bases (N-bases) added to culture medium at millimolar level result each in synthesis of the corresponding phospholipid via a de novo pathway at initial rates of 0.18, 0.30, and 0.36 nmol/h/micrograms DNA, respectively. Addition of methyl-labeled methionine to culture medium at tracer levels or at millimolar concentration enabled measurements of the rates of phospholipid methylation from EPG phosphatidylmonomethylaminoethanol (Me1EPG) and phosphatidyldimethylaminoethanol (Me2EPG) precursors. At tracer doses, the rates of methylation from the above respective phospholipids are 0.45, 1.17, and 1.70 pmol/h/micrograms DNA. At 1 mM methionine, synthesis of CPG proceeds from [14C]EPG or [14C]Me2EPG at initial rates of 8 and 17 pmol/h/micrograms DNA, respectively. Although the latter phospholipid analog can be generated from its monomethyl precursor, methylation of EPG does not result in the accumulation of Me2EPG, suggesting two segregated and metabolically distinct pathways. In the presence of N-bases, of the total [3H]serine incorporated into cellular phospholipids 30-36.5% of labelled SPG is converted into decarboxylation products. The decarboxylation and methylation routes contribute a significant portion of choline from endogenous sources, most likely through conversion of SPG.
The Relationship Between Knowledge of Recent HbA1c Values and Diabetes Care Understanding and Self-Management (2005)

Respondents who knew their HbA(1c) values reported better diabetes care understanding and assessment of their glycemic control than those who did not. Knowledge of one's HbA(1c) level alone, however, was not sufficient to translate increased understanding of diabetes care into the increased confidence and motivation necessary to improve patients' diabetes self-management. Strategies to provide information to patients must be combined with other behavioral strategies to motivate and help patients effectively manage their diabetes.
자폐 범주성 장애 아동을 대상으로 한 중심축 반응 훈련 중재의 메타분석 -단일대상 연구를 중심으로- (2012)

본 연구는 자폐 범주성 장애 아동을 대상으로 한 중심축 반응 훈련의 효과를 파악하는 것에 목적이 있다. 이를 위하여 관련 국내외 단일대상 연구 문헌을 조사하여 22편의 분석대상 문헌을 선정하여 검토하였고, 비중복 비율(PND) 기법을 이용하여 중재 효과크기를 분석하였다. 분석을 통한 주요 결과는 다음과 같다. 첫째, 중심축 반응 훈련 관련 연구는 1987년부터 2011년까지 지속적으로 진행되어 왔으며, 22편의 연구 중에서 18편이 국외 연구 이고, 4편이 국내 연구였다. 선정된 연구에 참여한 대상자의 수는 총 77명으로 그 중 남아가 55명, 여아가 11명으로 나타났다. 둘째, 중심축반응훈련 중재의 전반적인 효과크기는 중재와 일반화 단계 모두 중간 효과를 보이는 것으로 나타났다. 셋째, 각 변인에 따라 효과크기가 다르게 나타난 것도 있었다. 중재효과크기는, 연구자가 중재를 실행하였을 때, 종속변인이 아동의 문제행동 감소와, 부모의 중심축 반응 훈련 실행도 이었을 때, 중재가 매우 효과적인 것으로 나타났다. 이상의 분석결과를 토대로 자폐 범주성 장애 아동을 대상으로 한 중심축반응훈련 중재의 향후 연구 방향과 교육적 시사점이 논의되었다.