gebhart/patrepeval huggingface dataset:
B cell receptor triggering sensitizes human B cells to TRAIL-induced apoptosis (2010)

TRAIL is known to cause death in tumor cells, but physiological regulation of its activity remains poorly characterized. We demonstrate that BCR triggering sensitizes transformed centroblast-like BL cells and peripheral blood memory B cells to TRAIL-mediated apoptosis. The sensitization correlated with surface down-regulation and intracellular retention of TRAIL-R4, along with changes in the expression of several Bcl-2 protein family members. Although enhancing FAS-mediated cell death, CD40 activation protected B cells from TRAIL-induced apoptosis. Combination of Ig cross-linking with CD40 ligation did not prevent TRAIL-R4 down-regulation but induced changes in the mitochondria-regulated pathway of apoptosis that are known to be associated with resistance to TRAIL. Human CD5(+) B cells, presumably stimulated by reactivity to self without immunological help, exhibited very high ex vivo sensitivity to TRAIL. Our results define the first B-lymphocyte-specific physiological signal that increases cellular sensitivity to TRAIL. This may be important for our understanding of TRAIL involvement in the control of B cell responses and aid in designing TRAIL-based therapies for B cell lymphomas.
C-Slate: Exploring Remote Collaboration on Horizontal Multi-touch Surfaces (2007)

We introduce C-Slate, a new vision-based system, which utilizes stereo cameras above a commercially available tablet technology to support remote collaboration. The horizontally mounted tablet provides the user with high resolution stylus input, which is augmented by multi-touch interaction and recognition of untagged everyday physical objects using new stereo vision and machine learning techniques. This provides a novel and interesting interactive tabletop arrangement, capable of supporting a variety of fluid multi-touch interactions, including symmetric and asymmetric bimanual input, coupled with the potential for incorporating tangible objects into the user interface. When used in a remote context, these features are combined with the ability to see visual representations of remote users’ hands and remote physical objects placed on top of the surface. This combination of bimanual and tangible interaction and sharing of remote gestures and physical objects provides a new way to collaborate remotely, complementing existing channels such as audio and video conferencing.
Hereditary hemorrhagic telangiectasia: sonographic findings in the liver. (1984)

Three cases of hereditary hemorrhagic telangiectasia were evaluated by ultrasound. The sonographic findings included demonstration of an abnormally dilated common hepatic artery, multiple arteriovenous malformations, and abnormal echogenicity of the liver.
Dissemination of Cultural Heritage and Impact of Pilgrim Tourism at Gangasagar Island (2006)

Tourism plays a great role towards socio-economic changes. According to Indian sentiment the pilgrim centers or tirthasthan used to visit by number of tourists to earn virtue. Gangasagar is one of such centre where taking holy dip during makar sankranti, thought as, in life of a person earns that much of virtue which one can earn by visiting all the pilgrim centers throughout the life. The present paper aims to highlight the impact of pilgrim tourists at Gangasagar Island of Sundarban Biosphere and also projects the role of myth centering around Kapil Muni temple at Gangasagar which attracts not only tourists but also disseminate over the generation its cultural tenets which is valuable, being one of the repository of the great Indian cultural heritage.
NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer (2004)

It has been reported that two inducible prostaglandin synthetic enzymes, cylooxygenase-2 (COX-2) and microsomal PGE synthase, are over-expressed in non-small cell lung cancer (NSCLC). Using quantitative reverse transcription-polymerase chain reaction, we analyzed RNA levels of the key prostaglandin catabolic enzyme, NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH), in 19 pairs of NSCLC tumors and adjacent non-malignant tissue from the same patient. We found that 100% of tumor-tissue pairs showed at least a 2-fold decrease and 61% showed a 10-fold decrease. This suggests that the increased expression of COX-2 and PGE synthase in tumors may work in concert with the decreased expression of 15-PGDH to amplify an increase in tissue levels of proliferative PGE2. To further explore if 15-PGDH is related to tumorigenesis, athymic nude mice were injected with control A549 cells or cells transiently over-expressing wild-type or mutant 15-PGDH (Y151F). It was found that mice injected with control A549 cells or with cells expressing mutant enzyme produced tumors normally. However, mice injected with A549 cells expressing wild-type 15-PGDH had a significant decrease in tumor growth. Examining the effects of 15-PGDH expression on cellular changes in A549 cells, we found that over-expression of 15-PGDH induced apoptosis of A549 cells as evidenced by fragmentation of DNA, activation of pro-caspase 3, cleavage of poly(ADP-ribose) polymerase and decreased expression of Bcl-2. We also found that the expression of 15-PGDH was negatively related to that of pro-adhesive and invasive CD44. Furthermore, the expression of 15-PGDH was found to be stimulated by hyaluronidase. These results suggest that 15-PGDH may decrease the level of proliferative PGE2, induce apoptosis and function like a tumor suppressor.
Estrogen Receptor β Expression Is Associated with Tamoxifen Response in ERα-Negative Breast Carcinoma (2007)

Expression of ERbeta is an independent marker for favorable prognosis after adjuvant tamoxifen treatment in ERalpha-negative breast cancer patients and involves a gene expression program distinct from ERalpha. These results may be highly clinically significant, because in the United States alone, approximately 10,000 women are diagnosed annually with ERalpha-negative/ERbeta-positive breast carcinoma and may benefit from adjuvant tamoxifen.
OP9 Bone Marrow Stroma Cells Differentiate into Megakaryocytes and Platelets (2013)

Platelets are essential for hemostatic plug formation and thrombosis. The mechanisms of megakaryocyte (MK) differentiation and subsequent platelet production from stem cells remain only partially understood. The manufacture of megakaryocytes (MKs) and platelets from cell sources including hematopoietic stem cells and pluripotent stem cells have been highlighted for studying the platelet production mechanisms as well as for the development of new strategies for platelet transfusion. The mouse bone marrow stroma cell line OP9 has been widely used as feeder cells for the differentiation of stem cells into MK lineages. OP9 cells are reported to be pre-adipocytes. We previously reported that 3T3-L1 pre-adipocytes differentiated into MKs and platelets. In the present study, we examined whether OP9 cells differentiate into MKs and platelets using MK lineage induction (MKLI) medium previously established to generate MKs and platelets from hematopoietic stem cells, embryonic stem cells, and pre-adipocytes. OP9 cells cultured in MKLI medium had megakaryocytic features, i.e., positivity for surface markers CD41 and CD42b, polyploidy, and distinct morphology. The OP9-derived platelets had functional characteristics, providing the first evidence for the differentiation of OP9 cells into MKs and platelets. We then analyzed gene expressions of critical factors that regulate megakaryopoiesis and thrombopoiesis. The gene expressions of p45NF-E2, FOG, Fli1, GATA2, RUNX1, thrombopoietin, and c-mpl were observed during the MK differentiation. Among the observed transcription factors of MK lineages, p45NF-E2 expression was increased during differentiation. We further studied MK and platelet generation using p45NF-E2-overexpressing OP9 cells. OP9 cells transfected with p45NF-E2 had enhanced production of MKs and platelets. Our findings revealed that OP9 cells differentiated into MKs and platelets in vitro. OP9 cells have critical factors for megakaryopoiesis and thrombopoiesis, which might be involved in a mechanism of this differentiation. p45NF-E2 might also play important roles in the differentiation of OP9 cells into MK lineages cells.
A Hybrid Multichannel Hardware Simulator for Frequency-Selective Mobile Radio Paths (1983)

We present the analysis and design of an efficient hybrid digital/analog multichannel hardware simulator for frequencyselective Rayleigh-fading mobile radio paths. The simulator has eight RF outputs and is linear and reciprocal. All outputs exhibit mutually independent Rayleigh fading over a 45 dB range and have identical time delay spreads. Analog RF techniques are combined with a digital signal processor (DSP) to generate random path modulation. Frequency selectivity from time delay spread is created using several RF path delay elements. Simulated vehicle velocity is continuously variable from 6 to 60 mph. The simulator is thus a flexible tool for the study of mobile radio transmission techniques.
An analysis of rollover stability measurement for high-speed mobile robots (2006)

Mobile robots and passenger vehicles are frequently required to operate at high speeds, on terrain that is sloped or uneven. These systems can be susceptible to rollover, particularly during severe maneuvers. This paper presents an analysis of rollover stability measurement for mobile robots operating at high speeds. The analysis examines the accuracy of a commonly accepted rollover stability metric during operation on sloped and rough terrain. The effects of sensor placement, center-of-gravity position estimation error, and wheel dynamics are examined. It is shown that these effects can have a significant impact on stability measurement during high speed operation
Online directory of databases for material properties (1984)

We have created an online directory of databases of material properties on the Technology Information System at Lawrence Livermore National Laboratory (LLNL/TIS). This directory is intended to provide interactive access to scientific and technical databases available to the public that contain information pertaining to nuclear, atomic, molecular, physical, chemical, and mechanical properties of substances. The directory is based on work done earlier by Joseph Hilsenrath of the National Bureau of Standards (NBS/OSRD) and Jack H. Westbrook of General Electric Corporation. In addition to the 101 data files previously reported, we have updated the information and identified more than 38 new numeric databases and predictive systems in these fields. We have included, where applicable, entries contained in the directories published by Cuadra Associates, CODATA, and UNESCO. In addition to describing the contents of the databases, we have provided updated information on the availability of the databases and their online access over public telephone and data networks. The online directory is prepared for use by scientists and engineers and should enhance the sharing of S and T resources over communication networks. This directory is expected to become particularly important to the national and international magnetic- and laser-energy fusion projects, nuclear criticality safety,more » and computer aided engineering programs. Some of the numeric databases are directly accessible by authorized users via the TIS Intelligent Gateway Processor at LLNL (TIS/IGP), with self-guiding procedures for the downloading, merging, post-processing, and graphical/statistical analysis of data.« less
A novel Foxn1<sup>eGFP/+</sup> mouse model identifies Bmp4‐induced maintenance of <i>Foxn1</i> expression and thymic epithelial progenitor populations (2016)

Although forkhead-box n1 (Foxn1) is a critical thymic epithelial cell regulator in thymus organogenesis, its association with epithelial differentiation and homeostasis in the postnatal and aged thymic microenvironment remains conflicting. Consequently, we have generated a Foxn1<sup>eGFP/+</sup> knock-in mouse model that allows for refined investigation of the aging thymic epithelium. This reporter line differs from those previously published in that concomitant expression of enhanced green fluorescent protein enables live cell sorting of Foxn1<sup>+</sup> cell populations. Our heterozygotes did not exhibit haploinsufficiency, with Foxn1 expression resembling that of wild-type mice. Comparative analysis between Foxn1 and enhanced green fluorescent protein at both the transcriptional and translational levels revealed co-localization, with progressive down-regulation observed predominantly in the aging cortical epithelium. Supplementation with bone morphogenetic protein (Bmp)-4 enhanced Foxn1 expression and colony forming efficiency in both embryonic and adult progenitor 3D cultures. Strikingly, selective maintenance of immature cortical and medullary epithelial cells was observed which is consistent with the higher Bmp receptor 2 expression levels seen in these progenitor populations. This study demonstrates the significance of our mouse model in unraveling the role of this master regulator in thymus development, homeostasis and aging, providing a faithful reporter system for phenotypic and functional investigations.
On Denial of Service Attacks in Software Defined Networks (2016)

Software defined networking greatly simplifies network management by decoupling control functions from the network data plane. However, such a decoupling also opens SDN to various denial of service attacks: an adversary can easily exhaust network resources by flooding short-lived spoofed flows. Toward this issue, we present a comprehensive study of DoS attacks in SDN, and propose multi-layer fair queueing (MLFQ), a simple but effective DoS mitigation method. MLFQ enforces fair sharing of an SDN controller’s resources with multiple layers of queues, which can dynamically expand and aggregate according to controller load. Both testbed-based and emulation-based experiments demonstrate the effectiveness of MLFQ in mitigating DoS attacks targeted at SDN controllers.
Online electrochemistry/mass spectrometry in drug metabolism studies: principles and applications (2010)

The study of drug metabolism reactions in an electrochemical cell enables the prediction as well as the synthesis of oxidative metabolites. In particular, reactive metabolites are directly detected using online EC/electrospray ionization mass spectrometry. Thus, the electrochemical technique is a promising tool that complements existing in vivo and in vitro techniques in drug metabolism studies.
The Politics of Secondary Industry Research in Australia 1926-1939 (1989)

Historical Records of Australian Science publishes peer-reviewed articles and book reviews on the history of science and scientists in Australia and the southwest Pacific, biographical memoirs of deceased Fellows of the Academy, and an annual bibliography of the history of Australian science
Biosensors based on nanomechanical systems (2012)

The advances in micro- and nanofabrication technologies enable the preparation of increasingly smaller mechanical transducers capable of detecting the forces, motion, mechanical properties and masses that emerge in biomolecular interactions and fundamental biological processes. Thus, biosensors based on nanomechanical systems have gained considerable relevance in the last decade. This review provides insight into the mechanical phenomena that occur in suspended mechanical structures when either biological adsorption or interactions take place on their surface. This review guides the reader through the parameters that change as a consequence of biomolecular adsorption: mass, surface stress, effective Young's modulus and viscoelasticity. The mathematical background needed to correctly interpret the output signals from nanomechanical biosensors is also outlined here. Other practical issues reviewed are the immobilization of biomolecular receptors on the surface of nanomechanical systems and methods to attain that in large arrays of sensors. We then describe some relevant realizations of biosensor devices based on nanomechanical systems that harness some of the mechanical effects cited above. We finally discuss the intrinsic detection limits of the devices and the limitation that arises from non-specific adsorption.
Tumor-specific overexpression of a novel keratinocyte lipid-binding protein. Identification and characterization of a cloned sequence activated during multistage carcinogenesis in mouse skin (1993)

Differential screening of cDNA libraries from chemically induced malignant mouse skin squamous cell carcinomas (SCCs) identified sequences, including one called mal1, that were up-regulated in their expression at both the benign papilloma and the malignant SCC stages during tumor development. The mal1 plasmid cDNA clone was used to screen lambda phage cDNA libraries made from chemically induced papillomas and SCCs. Two size classes (655 and 933 nucleotides excluding the poly(A) tail) of full-length cDNAs were isolated. The corresponding mRNAs differ in their 3'-untranslated region by 278 nucleotides as a result of utilizing two alternative polyadenylation signals. Both transcripts were expressed simultaneously, showing the same expression patterns, with the smaller one being the predominant species. Most tissues examined showed a weak expression of mal1 mRNA. High levels of mal1 transcripts could be detected in adipose and mammary tissues and tongue epithelia and predominantly in epidermis. The expression observed in epidermis was up-regulated dramatically during tumor formation. Computer-assisted sequence analysis revealed one open reading frame that encoded a protein of 135 amino acid residues with extensive homology to members of the lipid-binding protein family. Residues determining the proposed beta-clam structure of these proteins and the structure of the lipid-binding region were shown to be conserved in the mal1 gene. In vitro translation of mal1 RNA yielded a polypeptide of the predicted size of 15 kDa that was immunoprecipitable with an anti-rat liver fatty acid-binding protein antiserum. Based on the sequence analysis and antigenic properties of mal1, we conclude that it encodes a novel member of the lipid-binding protein family.
Image compression using a feedforward neural network (2002)

We present an image compression technique using a feedforward neural network. The neural network has three layers: one input layer, one hidden layer and one output layer. The inputs of the neural network are original image data, while the outputs are reconstructed image data which are close to the inputs. If the amount of data required to store the hidden unit values and the connection weights to the output layer of the neural network is less than the original data, compression is achieved. In our experiments, we achieved a compression ratio of about 10 with good reconstructed image quality. The neural network construction algorithm we used has an attractive feature that each addition of a hidden unit to the network will always improve the image quality. Thus our compression scheme is flexible in the sense that the user can trade between image quality and compression ratio depending on the application requirements.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Magnesium and therapeutics. (1994)

Two different types of therapy with magnesium are used: physiological oral magnesium supplementation which is totally atoxic since it palliates magnesium deficiencies by simply normalizing the magnesium intake and pharmacological magnesium therapy which may induce toxicity since it creates iatrogenic magnesium overload. Primary and secondary magnesium deficiencies constitute the sole indication of physiological oral magnesium therapy. It is therefore necessary to be well acquainted with the clinical and paraclinical pattern of magnesium deficit and to discriminate between magnesium deficiency due to an insufficient magnesium intake which only requires oral physiological supplementation and magnesium depletion related to a dysregulation of the control mechanisms of magnesium status which requires more or less specific regulation of its causal dysregulation. Physiological oral magnesium load constitutes the best tool for diagnosis of magnesium deficiency and the first step of its treatment. Physiological oral magnesium supplementation (5 mg/kg/day) is easy and can be carried out in the diet or with magnesium salts, with practically only one contra-indication: overt renal failure. Specific and aspecific treatments of magnesium depletion are tricky using for example magnesium sparing diuretics, pharmacological doses of vitamin B6, physiological doses of vitamin D and of selenium. In order to use the pharmacological properties of induced therapeutic hypermagnesaemia, high oral doses of magnesium (> 10 mg/kg/day) are advisable for chronic indications and the parenteral route is suitable for acute indications. There are 3 types of indications: specific (for the treatment of some forms of magnesium deficit i.e. acute), pharmacological (i.e. without alterations of magnesium status) and mixed--pharmacological and aetiopathogenic--(for example complications of chronic alcoholism). Today pharmacological magnesium therapy mainly concerns the obstetrical, cardiological and anaesthesiological fields. The main indications are eclampsia, some dysrhythmias (torsades de pointe particularly) and myocardial ischaemias. But it is now difficult to situate the exact place of the pharmacological indications of magnesium. Magnesium infusions can only be envisaged in intensive care units with careful monitoring of pulse, arterial pressure, deep tendon reflexes, hourly diuresis, electrocardiogram and respiratory recordings. High oral magnesium doses besides their laxative action may bring latent complications which may reduce lifespan. There may remain some indications of the laxative and antacid properties of non soluble magnesium, particularly during intermittent haemodialysis. Lastly local use of the mucocutaneous and cytoprotective properties of magnesium is still valid, in cardioplegic solutions and for preservation of transplants particularly.
Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation) (2012)

We have developed a new multimodal molecular imaging system that combines CARS (coherent anti-Stokes Raman scattering), SHG (second harmonic generation), THG (third harmonic generation) and multiplex TSFG (third-order sum frequency generation) using a subnanosecond white-light laser source. Molecular composition and their distribution in living cells are clearly visualized with different contrast enhancements through different mechanisms of CARS, SHG, THG and TSFG. A correlation image of CARS and TSF reveals that the TSF signal is generated predominantly from lipid droplets inside a cell as well as the peripheral cell wall.