gebhart/patrepeval huggingface dataset:
Molecular Mechanism in<i>α</i>-Glucosidase and Glucoamylase (1997)

The hydrolysis of glucosidic linkage catalyzed by every carbohydrate-hydrolase is a reaction in which the product retains (alpha-->alpha or beta-->beta) or inverts (alpha-->beta or beta-->alpha) the anomeric configuration of the substrate. alpha-Glucosidase and glucoamylase are essentially distinguished by releasing alpha-glucose and beta-glucose, respectively, from the common substrates having alpha-glucosidic linkage. The distinction in the substrate specificities of the two enzymes was explained by the subsite affinities in their active sites. The amino acid sequences of the regions containing the catalytic sites were compared in alpha-glucosidases and glucoamylases from various sources. alpha-Glucosidases were suggested to be grouped into two families by their primary structures. The catalytic reaction mechanisms of carbohydrate-hydrolases were discussed in the two significant models of a nucleophilic displacement mechanism and an oxocarbenium ion intermediate mechanism.
Characteristic Component of Rehmanniae Radix Preparata Compared to Rehmanniae Radix and Rehmanniae Radix Crudus (1993)

Rehmanniae Radix Preparata is manufactured with Rehmanniae Radix accoding to KP V. For quality control of Rehmanniae Radix Preparata, its standard component is required. The methanol extracts of Rehmanniae Radix crudus, Rehmanniae Radix, Rehmanniae Radix preparata were divided into the three groups of ether, butanol and aqueous fraction by liquid-liquid separation. In the comparative TLC of ether fraction, the characteristic component of Rehmanniae Radix preparata was found. The ether fraction was evaporated and separated on the silica gel column with chloroform-methanol and further separated on the preparative silica gel TLC with chloroform-methanol-water. The component was illucidated as 5-(hydroxymethyl)-2-furancarboxaldehyde(5-HMF). 5-HMF was not found in Rehmanniae Radix crudus and found in Rehmanniae radix in much less Quantities than Rehmanniae Radix Preparata.
Early stages in Bacillus subtilis transformation: association between homologous DNA and surface structures (1978)

The addition of ethylenediaminetetraacetate to competent cultures of Bacillus subtilis irreversibly inhibited the transformability as well as the cellular binding of DNA. Our results show that the inhibition of DNA binding by ethylenediaminetetraacetate in whole cells, protoplasts, and membrane vesicles is mainly due to a permanent alteration of the DNA receptors. Transformation absolutely requires free magnesium ions, whereas DNA binding is a magnesium-independent step. In contrast to ethylenediaminetetraacetate, the absence of Mg2+ does not irreversibly affect the capacity of the competent cells to be transformed DNA-binding receptors located at the cell surface remain associated with the plasma membrane after protoplasting and after isolation of membrane vesicles. A Mg2+-dependent endonucleolytic activity associated with the membrane appears to be responsible for the lower levels of binding by protoplasts in the presence of this ion.
Excursions in drug discovery (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExcursions in drug discoveryArthur A. PatchettCite this: J. Med. Chem. 1993, 36, 15, 2051–2058Publication Date (Print):July 1, 1993Publication History Published online1 May 2002Published inissue 1 July 1993https://pubs.acs.org/doi/10.1021/jm00067a001https://doi.org/10.1021/jm00067a001research-articleACS PublicationsRequest reuse permissionsArticle Views477Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Influence of particles on AC and DC electrical performance of gas insulated systems at extra-high-voltage (1977)

High-voltage breakdown measurements were made in two similar particle contaminated coaxial test systems, one with AC and the other with DC voltages. Information is presented on the effects of particle size, shape, and material for both SF <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">6</inf> and N <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> gases at pressures up to 15 atm in a plain coaxial gap and a coaxial gap including a post-type support spacer. Particle motion and location were found to strongly influence insulation performance. Measured values of electric fields which lifted and drove the particles, so that they bounced vertically and laterally, compare favorably with calculated levels. Movement into the the higher stress region at the center conductor was correlated with the initiation of sparkover. These breakdowns could be at levels more than a factor of five lower than those obtained when contamination was not introduced. Large variations in breakdown voltage of as much as 3 to 1 encountered under DC correspond to conditions where particle motion could be restricted, presumably by corona discharge, to motion near the outer electrode. AC sparkover levels were typically at the lower limits of the DC range. Both free and attached particles on the dielectric spacer surface would trigger flashover at the same low levels as were measured in the gas gap.
The Stony Brook / SMARTS Atlas of mostly Southern Novae (2012)

We introduce the Stony Brook / SMARTS Atlas of (mostly) Southern Novae. This atlas contains both spectra and photometry obtained since 2003. The data archived in this atlas will facilitate systematic studies of the nova phenomenon and correlative studies with other comprehensive data sets. It will also enable detailed investigations of individual objects. In making the data public we hope to engender more interest on the part of the community in the physics of novae. The atlas is on-line at \url{http://www.astro.sunysb.edu/fwalter/SMARTS/NovaAtlas/} .
Independent Association Between Plasma Leptin and C-Reactive Protein in Healthy Humans (2004)

Leptin and CRP levels are independently associated in normal humans, providing further evidence linking metabolic and inflammatory cardiovascular disease mechanisms.
Internet based infant monitoring system (2003)

The development of a prototype Internet-based infant monitoring system is currently ongoing. Real time analog, digital and video signals are transferred over the Internet. This project will investigate the advantages, disadvantages and limitations of using Internet for telemedicine monitoring.
A simple organ culture model for assessing the effects of growth factors on corneal re-epithelialization (1997)

The effects of growth factors on re-epithelialization of wounded human and bovine corneas were studied in a simple organ culture system. Excisional trephine and epithelial scrape wounds were created on bovine and human corneo-scleral rings in which the endothelial corneal concavity was then filled with an agar-collagen mixture. Organ culture was undertaken at 37 degrees C in a humidified 5% CO2 incubator with serum-free Medium 199 maintained at the level of the conjunctival epithelium. Rates of reepithelialization in response to addition of exogenous epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and transforming growth factor type beta 1 (TGF-beta 1) were assessed by image analysis. Corneal cultures could be maintained for up to 3 weeks without significant stromal oedema or keratocyte deterioration and with little loss of epithelial architecture. Following wounding the cornea reepithelialized in a similar fashion to that observed in vivo i.e. a lag phase followed by migration/proliferation and the reformation of an intact multilayered epithelium. EGF accelerated, basic FGF had no effect on, and TGF-beta 1 inhibited the rate of corneal re-epithelialization. Our organ culture model maintains corneal integrity and provides a practical system in which to study factors that modulate corneal reepithelialization following wounding.
Nucleic Acid Homologies Among Oxidase-Negative <i>Moraxella</i> Species (1970)

The deoxyribonucleic acid (DNA) base composition and DNA homologies of more than 40 strains of oxidase-negative Moraxella species were determined. These bacteria have also been identified as belonging to the Mima-Herellea-Acinetobacter group and the Bacterium anitratum group, as well as to several other genera including Achromobacter and Alcaligenes. The DNA base content of these strains ranged from 40 to 46% guanine plus cytosine. DNA-DNA competition experiments distinguished five groups whose members were determined by showing 50% or more homology to one of the reference strains: B. anitratum type B5W, Achromobacter haemolyticus var. haemolyticus, Alcaligenes haemolysans, Achromobacter metalcaligenes, and Moraxella lwoffi. A sixth group comprised those strains showing less than 50% homology to any of the reference strains. Negligible homology was found between strains of oxidase-negative and oxidase-positive Moraxella species in DNA-DNA competition experiments. However, evidence of a distant relationship between the two groups was obtained in competition experiments by using ribosomal ribonucleic acid.
A new method for the rapid titrimetric analysis of sodium alkyl sulphates and related compounds (1948)

S. R. Epton, Trans. Faraday Soc., 1948, 44, 226 DOI: 10.1039/TF9484400226
Polar localization of the MinD protein of <i>Bacillus subtilis</i> and its role in selection of the mid-cell division site (1998)

Cell division in rod-shaped bacteria is initiated by formation of a ring of the tubulin-like protein FtsZ at mid-cell. Division site selection is controlled by a conserved division inhibitor MinCD, which prevents aberrant division at the cell poles. The Bacillus subtilis DivIVA protein controls the topological specificity of MinCD action. Here we show that DivIVA is targeted to division sites late in their assembly, after some MinCD-sensitive step requiring FtsZ and other division proteins has been passed. DivIVA then recruits MinD to the division sites preventing another division from taking place near the newly formed cell poles. Sequestration of MinD to the poles also releases the next mid-cell sites for division. Remarkably, this mechanism of DivIVA action is completely different from that of the equivalent protein MinE of Escherichia coli, even though both systems operate via the same division inhibitor MinCD.
Auditory Temporal and Spectral Resolution in Normal and Impaired Hearing (1999)

Temporal, spectral, and combined temporal-spectral resolution of hearing was assessed by recording masked hearing thresholds. The masker was an octave band noise. Spectral resolution was assessed by introducing a spectral gap of half an octave bandwidth in the masker. A 50-msec gap assessed temporal resolution. The spectral and temporal gaps were used separately or simultaneously. Normal-hearing and hearing-impaired subjects participated. For each masking condition, the subjects were tested at masker levels 50, 60, 70, and 80 dB SPL and at test-tone frequencies 0.5, 1, 2, and 4 kHz. Normal-hearing subjects showed reduced masking with spectral and temporal gaps. The combination of spectral and temporal gap reduced masking further. The release of masking was dependent upon the masker level. Hearing-impaired subjects showed less release of masking than normal-hearing subjects. The degree of hearing impairment was inversely related to release of masking. Reliability of the test procedure was assessed.
Generalized Krylov recycling methods for solution of multiple related linear equation systems in electromagnetic analysis (2008)

In this paper we propose methods for fast iterative solution of multiple related linear systems of equations. Such systems arise, for example, in building pattern libraries for interconnect parasitic extraction, parasitic extraction under process variation, and parameterized interconnect characterization. Our techniques are based on a generalized form of "recycled" Krylov subspace methods that use sharing of information between related systems of equations to accelerate the iterative solution. Experimental results on electromagnetics problems demonstrate that the proposed method can achieve a speed-up of 5X~30X compared to direct GMRES applied sequentially to the individual systems. These methods are generic, fully treat nonlinear perturbations without approximation, and can be applied in a wide variety of application domains outside electromagnetics.
Testing the Implementation of Clinical Guidelines (1991)

come achieved eventually led many to call for national efforts to employ professional education in addition to financial incentives to promote more cost-effective practice.2 Unfortunately, it was not at all clear which clinical approaches merited advocacy. Less than 20 percent of all commonly accepted clinical modalities had been demonstrated to be efficacious with a randomized controlled trial (RCT).3 Because the cost of performing an RCT for all of the remaining tests, procedures, and medicines now in use would be prohibitive, observational designs were promoted as an acceptable alternative for providing physicians with the information urgently needed to guide their practice.4 Consequently, the Agency for Health Care Policy and Research (AHCPR) was established in December 1989 and charged with responsibility for the conduct of such the development of clinical guidelines based on the findings of outcome research, the dissemination of guidelines to the health care community, and the evaluation of the effects of
Cellular immune responses induced in cattle by heterologous prime–boost vaccination using recombinant viruses and bacille Calmette–Guérin (2004)

The development of novel vaccine strategies to replace or supplement bacille Calmette-Guérin (BCG) is urgently required. Here we study, in cattle, the use of heterologous prime-boost strategies based on vaccination with BCG and the mycobacterial mycolyl transferase Ag85A (Rv3804c) expressed either in recombinant modified vaccinia virus Ankara (MVA85A) or attenuated fowlpox strain FP9 (FP85A). Five different vaccination schedules were tested in the first experiment: MVA85A followed by BCG (group 1); BCG followed by MVA85A (group 2); BCG followed by FP85A and then MVA85A (group 3); MVA85A followed by MVA85A and then FP85A (group 4); and FP85A followed by FP85A and then MVA85A (group 5). Vaccine-induced levels of cellular immunity were assessed by determining interferon-gamma (IFN-gamma) responses in vitro. Prime-boost protocols, using recombinant MVA and BCG in combination (groups 1-3), resulted in significantly higher frequencies of Ag85-specific IFN-gamma-secreting cells than the two viral vectors used in combination (P=0.0055), or BCG used alone (groups 2 and 3, P=0.04). The T-cell repertoires of the calves in all five groups were significantly broader following heterologous booster immunizations than after the primary immunization. In a second experiment, the effects of BCG\MVA85A heterologous prime-boost vaccination were compared with BCG\BCG homologous revaccination. The results suggested a higher Ag85A-specific response with a wider T-cell repertoire in the MVA85A-boosted calves than in the BCG\BCG-vaccinated calves. In conclusion therefore, the present report demonstrates the effectiveness of heterologous prime-boost strategies based on recombinant MVA and BCG to induce strong cellular immune responses in cattle and prioritise such vaccination strategies for rapid assessment of protective efficacy in this natural target species of tuberculosis.
Efficacy in Pigs of Inactivated and Live Attenuated Influenza Virus Vaccines against Infection and Transmission of an Emerging H3N2 Similar to the 2011-2012 H3N2v (2013)

Vaccines provide a primary means to limit disease but may not be effective at blocking infection and pathogen transmission. The objective of the present study was to evaluate the efficacy of commercial inactivated swine influenza A virus (IAV) vaccines and experimental live attenuated influenza virus (LAIV) vaccines against infection with H3N2 virus and subsequent indirect transmission to naive pigs. The H3N2 virus evaluated was similar to the H3N2v detected in humans during 2011-2012, which was associated with swine contact at agricultural fairs. One commercial vaccine provided partial protection measured by reduced nasal shedding; however, indirect contacts became infected, indicating that the reduction in nasal shedding did not prevent aerosol transmission. One LAIV vaccine provided complete protection, and none of the indirect-contact pigs became infected. Clinical disease was not observed in any group, including nonvaccinated animals, a consistent observation in pigs infected with contemporary reassortant H3N2 swine viruses. Serum hemagglutination inhibition antibody titers against the challenge virus were not predictive of efficacy; titers following vaccination with a LAIV that provided sterilizing immunity were below the level considered protective, yet titers in a commercial vaccine group that was not protected were above that level. While vaccination with currently approved commercial inactivated products did not fully prevent transmission, certain vaccines may provide a benefit by limitating shedding, transmission, and zoonotic spillover of antigenically similar H3N2 viruses at agriculture fairs when administered appropriately and used in conjunction with additional control measures.
Comparison of On-Wafer Calibrations (1991)

A powerful new verification technique determines the measurement accuracy of scattering parameter calibrations. The technique determines the relative reference impedance, reference plane offset, and the worst-case measurement deviations of any calibration from a benchmark calibration. The technique is applied to several popular on-wafer scattering parameter calibrations, and the deviations between those calibrations and the thru-reflect line calibration are quantified.
Improvement of actuation displacement of LIPCA implementing bifurcation phenomena (2006)

In this work, behavior of a unimorph piezoceramic actuator, LIPCA (Lightweight Piezo-Composite Actuator) under compression has been experimentally and numerically investigated. The LIPCA composed of composite laminated tabs, piezoceramic material layer, glass/epoxy composite and carbon fiber composite layers was modeled and analyzed by using a full three-dimensional finite element modeling technique. The geometrically nonlinear analysis was used in the analysis because the LIPCA has the initial curvature due to the curing process, which acts like an initial geometric imperfection. The LIPCA was installed in the simply supported configuration and compressive load was applied in the test jig. By measuring the lateral displacement created by the compressive load, the buckling load of the LIPCA was determined. The measured buckling load agreed well with the computed linear buckling load from the finite element analysis based on the thermal analogy. As various electric fields were applied to the LIPCA under the compressive load, the lateral displacement was measured to examine behavior of the LIPCA under the compressive load and electric field at the same time. From this test, proper combinations of the compressive load and prescribed voltage could be figured out, which can create controlled buckling of the LIPCA under compression by applying the electric field. The measured data showed that the lateral displacement of the LIPCA is significantly increased when a proper electric field is prescribed to the LIPCA in addition to the pre-determined compressive load. The measured data was compared with the computed result from the geometrically nonlinear finite element analysis based on the thermal analogy. The numerical simulation agreed well with the measurement for low compressive load (< 3N) and low electric field (< 150V). The strength of the LIPCA is also calculated to make sure that the actuator can be operated without fracture.
Designing an Analytics Platform for Professional Sports Teams (2015)

Professional sports is an area with huge economical and societal impact. However, so far it has received rather little attention by information systems research. Therefore, we design an Analytics Platform for Professional Sports Teams. We introduce Sports Analytics as an interdisciplinary research field and establish a design science research project. As part of this project, we first explore three professional sports teams and theorize meta-requirements for our platform. Second, we propose design principles that indicate how the proposed meta-requirements may be addressed. Third, we develop a prototypical web application that indicates how the proposed design principles may be instantiated. Finally, we evaluate the application with two professional sports teams and a global software vendor. As part of this evaluation, we focus on relevance and feasibility of the proposed Analytics Platform for Professional Sports Teams. We conclude this article by discussing its implications.
The metabolism of fluoroacetate by plants (1969)

Research Article| June 01 1969 The metabolism of fluoroacetate by plants P F Ward; P F Ward 1Department of Biochemistry, Agricultural Research Council Inctitute of Animal Physiology, Babraham, Cambridge Search for other works by this author on: This Site PubMed Google Scholar N S Huskisson N S Huskisson 1Department of Biochemistry, Agricultural Research Council Inctitute of Animal Physiology, Babraham, Cambridge Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1969) 113 (2): 9P. https://doi.org/10.1042/bj1130009Pa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation P F Ward, N S Huskisson; The metabolism of fluoroacetate by plants. Biochem J 1 June 1969; 113 (2): 9P. doi: https://doi.org/10.1042/bj1130009Pa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1969 The Biochemical Society1969 Article PDF first page preview Close Modal You do not currently have access to this content.