gebhart/patrepeval huggingface dataset:
A Novel p53 Transcriptional Repressor Element (p53TRE) and the Asymmetrical Contribution of Two p53 Binding Sites Modulate the Response of the Placental Transforming Growth Factor-β Promoter to p53 (2002)

Previous studies in our laboratory and others identified placental transforming growth factor-beta (PTGF-beta) as an important downstream mediator of DNA damage signaling and a transcriptional target of p53. Here we show that accumulation of PTGF-beta mRNA in response to p53 overexpression is delayed relative to p21(WAF1), whereas the promoters of these genes respond to p53 with similar kinetics. Mutational analyses of two p53 binding sites within the PTGF-beta promoter revealed that site p53-1 (+29 bp) is responsible for as much as 80% of the transcriptional response to p53. This is consistent with electrophoretic mobility shift assays showing that site p53-1 binds p53 with a much higher affinity than site p53-2 (-850 bp). We also describe for the first time a novel 21-bp element (-222 to -242 bp) that acts to down-regulate the PTGF-beta promoter response to p53. Termed the p53 transcriptional repressor element (p53TRE), this sequence was shown to suppress p53 transactivation in a position- and promoter-independent fashion and to associate with a 28-kDa protein expressed in several tumor cell lines. A p53 suppressor element and asymmetric p53 binding sites may participate determining the activation thresholds of p53-responsive promoters in a cell- and context-specific manner.
CD157 is an important mediator of neutrophil adhesion and migration (2004)

CD157, a glycosylphosphatidylinositol (GPI)-anchored protein encoded by a member of the CD38 NADase/ADP-ribosyl cyclase gene family, is expressed on the surface of most human circulating neutrophils. This work demonstrates that CD157 is a receptor that induces reorganization of the cytoskeleton and significant changes in cell shape, and that signals mediated by CD157 act through modulation of cytosolic Ca(2+) concentration. These signals are independent of the products of CD157's enzymatic activities (ie, cyclic adenosine diphosphate [ADP]-ribose and ADP-ribose). Indeed, the enzymatic activities of CD157 in circulating neutrophils as well as in dimethyl sulfoxide (DMSO)-differentiated (CD157(+)/CD38(-)) HL-60 cells, are hardly detectable. This work also shows that the receptorial activity relies on cross-talk between CD157 and beta(2) integrin. CD157 localizes in GM1-enriched lipid rafts and, upon activation, it migrates to the uropod, a structure specialized in motility and adhesive functions. Indeed, CD157 is involved in adhesion to extracellular matrix proteins and in chemotaxis induced in vitro by formyl-methionyl-leucyl-phenylalanine (fMLP). These findings were consistent with the results obtained in neutrophils from patients with paroxysmal nocturnal hemoglobinuria (PNH), in which CD157 is deficient. These neutrophils showed constant defects in adhesion and migration. Our data attribute specific and crucial roles to CD157 in the regulation of innate immunity during inflammation.
Update on Irritable Bowel Syndrome Program of Research (2013)

The key to planning effective management strategies is to understand the heterogeneity of this disorder. Interventions for IBS include non-pharmacological strategies such as cognitive behavior therapy, relaxation strategies, and exclusion diets.
High-Throughput Screen for Small Molecule Inhibitors of Mint1-PDZ Domains (2007)

Several hundred PDZ (postsynaptic density-95, Drosophila disks-large, ZO-1) domain-containing proteins have been identified in the human genome. PDZ domains play a critical role in organization and function of cellular signaling pathways. Thus, small molecule inhibitors of PDZ domain association with their targets have wide potential applications as research and therapeutic agents. PDZ domains typically bind to a carboxyl-terminal tail of the target protein. Here we describe a high-throughput screening (HTS) assay for small molecule inhibitors of association between Mint1-PDZ domains and N-type Ca2+ channel carboxyl-terminal peptide (NC peptide). The performance of a homogeneous time-resolved fluorescence resonance energy transfer (HTRF) and an amplified luminescent proximity homogeneous assay (ALPHA) were systematically compared in parallel pilot HTS experiments with glutathione S-transferase-Mint1-PDZ1/2 protein and biotinylated NC peptide. Both of the two assays showed similar sensitivities in our target protein assay. Using HTRF-based assay we screened a library of 100,000 small molecule compounds and identified a number of potential "hits." The activity of isolated "hits" was confirmed by ALPHA assay. However, further evaluation revealed that isolated "hits" most likely act as "promiscuous binders," not as specific Mint-PDZ inhibitors, and that additional screening will be required to identify the true Mint-PDZ inhibitors. The assays described provided an example of HTS for a small molecule inhibitor of Mint-PDZ domain that can be easily adapted to other PDZ domain-mediated interactions.
Reconstruction and processing of medical electronic endoscopic image based on white LED illumination (2011)

A video laparo endoscopic system was introduced in this paper, which included a camera, a white LED illuminator and a high-definition image processing workstation. The camera adopted a CMOS image sensor to achieve high resolution imaging. Four LEDs were assembled directly to the front-end of the endoscope. Optical fiber coupling system was unnecessary. Image processing workstation was used to demosaic, enhance, and display the image on an LCD monitor in real time, and then capture and store the image. The image resolution reached 1280*1024. This system can be used directly in a laparoscope casing of 10mm in diameter. It is compact, low-cost, high-definition, and suitable for the single-port laparoscope surgery.
Rechargeable solid‐state Li‐air batteries: a status report (2018)

Abstract The theoretical specific energy of lithium‐air battery is as high as 3436 Wh·kg −1 , and the possible achieved value may reach 600–700 Wh·kg −1 , which enables this energy storage system as an important propulsion power sources for electric vehicles with the driving range of 500–800 km. Currently, Li‐air batteries are facing main challenges at stability, efficiency, applicability and safety. In particular, from a practical view of point, the Li‐air batteries should be operated directly in ambient air. Solid‐state battery system is the best avenue to eventually solve these main issues. At the heart of the solid state, Li‐air technology is the solid‐state Li + ‐conducting ceramic material. Developing solid‐state lithium‐air batteries (SSLAB) can solve the problem of applicability fundamentally and circumvent the safety issues completely, and it is also an important avenue to improve the stability of the battery system. In this paper, we provide a systematical review of the progress in the cell construction, the regulation of the electrode/electrolyte interface, the cell assembly, the electrochemical performance and the mechanism for the SSLAB. In every section, the contributions of the recent research progress in the main challenges and the remained questions will be commented. Based on these reviews, we attempt to propose some alternative approaches for the next stage and suggest a development prospective for the SSLAB.
Survey of Vitamin Content of Fortified Milk (1988)

This paper summarizes work done by 4 different laboratories on the vitamin content of milk. Riboflavin, vitamin A, and vitamin D were assayed in whole, 2%, and skim milks that had been fortified. In general, the adherence to label claim decreased with decreasing fat content. This may be due to methods and stage of vitamin addition prior to processing.
Studies of Inhibitory Effect of Ammonium Ions in Several Virus-Tissue Culture Systems (1961)

SummaryThe antiviral effect of ammonium ions. previously shown in dog kidney cells against influenza virus, has been reexamined with 10 viruses in 10 tissue culture cell lines. Only influenza virus appeared to be affected by the inhibitor, which functioned in a number of cell systems. The effect was shown to be independent of virus inoculum size and therefore apparently on the host cell. The inhibition was demonstrated in suspended allantoic membrane cultures, and a marginal effect was also observed in intact chick embryos.
A common neoepitope is created when the reactive center of C1-inhibitor is cleaved by plasma kallikrein, activated factor XII fragment, C1 esterase, or neutrophil elastase. (1988)

The reactive center of C1-inhibitor, a plasma protease inhibitor that belongs to the serpin superfamily, is located on a peptide loop which is highly susceptible to proteolytic cleavage. With plasma kallikrein, C1s and beta-Factor XIIa, this cleavage occurs at the reactive site residue P1 (Arg444); with neutrophil elastase, it takes place near P1, probably at residue P3 (Val442). After these cleavages, C1-inhibitor is inactivated and its conformation is modified. Moreover, in vivo, cleaved C1-inhibitor is removed from the blood stream more rapidly than the intact serpin, which suggests that proteolysis unmasks sites responsible for cellular recognition and the uptake of the cleaved inhibitor. In the study reported here, we show, using an MAb, that an identical neoepitope is created on C1-inhibitor after the cleavage of its exposed loop by plasma kallikrein, C1s, beta-Factor XIIa, and by neutrophil elastase.
Evaluation of Multi-Target and Single-Target Liposomal Drugs for the Treatment of Gastric Cancer (2008)

We studied the effects of multi- and single-target liposomal drugs on human gastric cancer cell AGS both in vitro and in vivo. The cytotoxic effect of dihydrotanshinone I was significantly enhanced by treatment with octreotide-polyethylene glycol(PEG)-liposome, Arg-Gly-Asp(RGD)-PEG-liposome, and RGD/octreotide-PEG-liposome encapsulated with 0.5 mug/ml of dihydrotanshinone I to AGS cell for 24 h, compared to control. Furthermore, the AGS cell survival rate for multi-target versus single target liposomal drugs was significantly suppressed. Microscopic examination revealed that significant cell death occurred in the multi- and single-target liposomal encapsulated drug groups. Significant suppression of tumor growth in AGS cell xenograft nude mice given octreotide-PEG-liposome, RGD/octreotide-PEG-liposome encapsulated drug, versus those given a free drug was noted after 13 d of experimentation with the multi-targeted liposome: up to 60.75% and 41.2% reduction of tumor volume as compared to dimethylsulfoxide (DMSO) control and the free drug groups respectively. The treated animals showed no gross signs of toxicity. The results have potential clinical application.
Equivalent regulation of wild type and an epitope‐tagged variant of G<sub>s</sub>α by the IP prostanoid receptor following expression in neuroblastoma × glioma hybrid, NG108‐15, cells (1994)

NG108-15 cells were transfected to stably express a haemagglutinin epitope-tagged variant of the long isoform of Gs alpha. Clone BST15 expressed this polypeptide at similar levels to the endogenous long isoform of Gs alpha. Treatment of clone BST15 with the IP prostanoid receptor agonist iloprost resulted in down-regulation of both forms of Gs alpha with both dose-effect curves to iloprost and time courses of loss of the two forms of Gs alpha being indistinguishable. These results demonstrate that the IP prostanoid receptor interacts with and regulates the epitope-tagged variant of Gs alpha in an equivalent manner to the unmodified protein and indicates that the epitope-tagged polypeptide can be used to analyse mechanisms of receptor regulation of cellular G-protein levels.
Influence of Different Allelic Variants of the <i>CYP3A</i> and <i>ABCB1</i> Genes on the Tacrolimus Pharmacokinetic Profile of Chinese Renal Transplant Recipients (2006)

Tacrolimus has a narrow therapeutic window and a wide interindividual variation in its pharmacokinetics. The cytochrome P450 3A (CYP3A) and the ATP-binding cassette B1 (ABCB1) genes play an important role in the tacrolimus disposition. Therefore, the aim of this study was to evaluate whether CYP3A and ABCB1 polymorphisms are associated with the area under the time concentration curve (AUC0-12) calculated using a two time point sample strategy. The CYP3A and ABCB1 genotypes were determined by real-time polymerase chain reaction (RT-PCR) fluorescence resonance energy transfer (FRET) assays in 103 Chinese renal transplant recipients and consequently related to their dose-normalized (dn)AUC0-12. A significant allele-dependent effect (Kruskal-Wallis; p < 0.001) was observed between the CYP3A5*3 polymorphism and the dnAUC0-12. Multiple regression analysis showed that the CYP3A5*3 polymorphism is the most significant independent variable and explained 35% of the dose requirement variability in relation to tacrolimus use. Regarding the ABCB1 G2677T/A and C3435T polymorphisms, a trend was observed between the different genotypes and the dnAUC0-12. In conclusion, the CYP3A5*3 polymorphism may be an important factor in determining the dose requirement for tacrolimus and genotyping can help determine the initial daily dose required by individual patients for adequate immunosuppression.
Distributed Automatic Modulation Classification Based on Cyclic Feature via Compressive Sensing (2013)

Automatic modulation classification (AMC) is an important component in cognitive radio and many efforts have been made to improve the AMC's successful classification rate, especially when the environment is noisy. The cyclic feature has excellent resiliency to noise, so it has been frequently adopted as the feature for AMC. In this paper, in order to enhance the reliability of the system, we propose a distributed AMC scheme based on compressive sensing by taking advantage of the sparse property of cyclic feature map. A novel method based on compressive sensing principle for capturing the prominent peaks of the feature map is introduced, which is able to achieve good modulation recognition performance at sub-Nyquist rates. And a novel neural network fusion strategy is proposed for better cooperation. It is shown that the proposed distributed approach improves the classification rate compared with the single radio and can reduce the required samples compared with traditional sampling scheme.
Cat2Vec: Learning Distributed Representation of Multi-field Categorical Data (2017)

This paper presents a method of learning distributed representation for multi-field categorical data, which is a common data format with various applications such as recommender systems, social link prediction, and computational advertising. The success of non-linear models, e.g., factorisation machines, boosted trees, has proved the potential of exploring the interactions among inter-field categories. Inspired by Word2Vec, the distributed representation for natural language, we propose Cat2Vec (categories to vectors) model. In Cat2Vec, a low-dimensional continuous vector is automatically learned for each category in each field. The interactions among inter-field categories are further explored by different neural gates and the most informative ones are selected by pooling layers. In our experiments, with the exploration of the interactions between pairwise categories over layers, the model attains great improvement over state-of-the-art models in a supervised learning task, e.g., click prediction, while capturing the most significant interactions from the data.
[Fear memory extinction accelerated by simultaneous activation of GABA(B) and NMDA receptors]. (2012)

Effects of the N-methyl-D-aspartate (NMDA) agonist (D-cycloserine) and GABA(B) agonist (baclofen) on the fear memory extinction as manifested in the passive avoidance reaction in C57Bl/6J mice with "behavioral despair" (model of depression) and without previously developed stress have been studied. The positive acceleration of the fear memory extinction was observed in depressive mice upon the simultaneous administration of D-cycloserine and baclofen.
ChemInform Abstract: Structure‐Activity Relationships of Taxol®: Synthesis and Biological Evaluation of C2 Taxol Analogues. (1994)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Monocyclic pteridine analogues. A pyrazine analogue of methotrexate (1985)

Abstract Reaction of aminocyanoacetamide with bromomethylpyruvaldoxime provided 3‐amino‐6‐bromomethyl‐2‐carbamoylpyrazine 4‐oxide (7) , which was converted to a pyrazine analogue (3) of methotrexate by subsequent condensation with diethyl N ‐[4‐(methylamino)benzoyl]‐L‐glutamate, deoxygenation, and hydrolysis. The methotrexate analogue 3 was a poor inhibitor (I 50 > 150 μM) of bacterial or mammalian dihydrofolate reductase.
$\lambda$-NIC: Interactive Serverless Compute on Programmable SmartNICs (2019)

There is a growing interest in serverless compute, a cloud computing model\nthat automates infrastructure resource-allocation and management while billing\ncustomers only for the resources they use. Workloads like stream processing\nbenefit from high elasticity and fine-grain pricing of these serverless\nframeworks. However, so far, limited concurrency and high latency of server\nCPUs prohibit many interactive workloads (e.g., web servers and database\nclients) from taking advantage of serverless compute to achieve high\nperformance.\n In this paper, we argue that server CPUs are ill-suited to run serverless\nworkloads (i.e., lambdas) and present $\\lambda$-NIC, an open-source framework,\nthat runs interactive workloads directly on a SmartNIC; more specifically an\nASIC-based NIC that consists of a dense grid of Network Processing Unit (NPU)\ncores. $\\lambda$-NIC leverages SmartNIC's proximity to the network and a vast\narray of NPU cores to simultaneously run thousands of lambdas on a single NIC\nwith strict tail-latency guarantees. To ease development and deployment of\nlambdas, $\\lambda$-NIC exposes an event-based programming abstraction,\nMatch+Lambda, and a machine model that allows developers to compose and execute\nlambdas on SmartNICs easily. Our evaluation shows that $\\lambda$-NIC achieves\nup to 880x and 736x improvements in workloads' response latency and throughput,\nrespectively, while significantly reducing host CPU and memory usage.\n
Digital oxide deposition of SiO2 layers for III-nitride metal-oxide-semiconductor heterostructure field-effect transistors (2006)

We present a digital-oxide-deposition (DOD) technique to deposit high quality SiO2 dielectric layers by plasma-enhanced chemical vapor deposition using alternate pulses of silicon and oxygen precursors. The DOD procedure allows for a precise thickness control and results in extremely smooth insulating SiO2 layers. An insulating gate AlGaN∕GaN heterostructure field-effect transistor (HFET) with 8nm thick DOD SiO2 dielectric layer had a threshold voltage of −6V (only 1V higher than that of regular HFET), very low threshold voltage dispersion, and output continuous wave rf power of 15W∕mm at 55V drain bias.
p8 Is a New Target of Gemcitabine in Pancreatic Cancer Cells (2006)

Gemcitabine is the only available chemotherapeutic treatment of pancreatic cancers. It is, however, moderately effective, showing a tumor response rate of only 12%. The aim of this work was to identify new pathways involved in the resistance of pancreatic cancer cells to gemcitabine, in the hope of developing new adjuvant strategies to enhance its therapeutic efficacy. Comparison of gene expression patterns of five human pancreatic cancer cell lines showing different degrees of resistance to gemcitabine revealed specific overexpression of several genes in the most resistant. One of them encoded the antiapoptotic p8 protein. We found that (a) knocking down p8 expression in gemcitabine-resistant cells promoted cell death and increased caspase-3 activity; (b) forced overexpression of p8 in gemcitabine-sensitive cells increased their resistance to gemcitabine-induced apoptosis; and (c) gemcitabine down-regulated p8 mRNA expression. These results suggest that, in pancreatic cancer cells, a large part of gemcitabine-induced apoptosis results from the inhibition of the constitutive antiapoptotic activity of p8. Hence, targeting the p8-associated pathway could be a new adjuvant therapy improving the response of patients with pancreatic cancer to gemcitabine treatment.
A joint adaptive beamforming and user scheduling algorithm for downlink network MIMO systems (2013)

In this paper, we study multiple-input single-output downlink cellular systems which jointly design adaptive inter-cell interference cancellation and user scheduling assuming that partial channel state information (CSI) is shared among base stations (BSs). Since the optimal solution requires high complexity, we propose a new low complexity algorithm which selects the best users and their beamforming (BF) strategies in terms of maximizing the weighted sum rate. To this end, we first develop a simple threshold criterion for each user to decide the preferred BF strategy based on the derivation of the expected signal-to-interference-plus-noise ratio. Then, according to users' feedback about their decisions, a successive user and BF selection algorithm is performed at the BSs. From simulation results, we show that combined with proportional fair scheduling, the proposed scheme provides excellent sum rate performance with very low computational complexity.
.alpha.-Lithioamine synthetic equivalents: syntheses of diastereoisomers from Boc derivatives of cyclic amines (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT.alpha.-Lithioamine synthetic equivalents: syntheses of diastereoisomers from Boc derivatives of cyclic aminesPeter Beak and Won Koo LeeCite this: J. Org. Chem. 1993, 58, 5, 1109–1117Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://pubs.acs.org/doi/10.1021/jo00057a024https://doi.org/10.1021/jo00057a024research-articleACS PublicationsRequest reuse permissionsArticle Views4169Altmetric-Citations244LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Space Remote Sensing Systems: An Introduction (1985)

Space Remote Sensing Systems: An Introduction discusses the space remote sensing system, which is a modern high-technology field developed from earth sciences, engineering, and space systems technology for environmental protection, resource monitoring, climate prediction, weather forecasting, ocean measurement, and many other applications. This book consists of 10 chapters. Chapter 1 describes the science of the atmosphere and the earth's surface. Chapter 2 discusses spaceborne radiation collector systems, while Chapter 3 focuses on space detector and CCD systems. The passive space optical rad