gebhart/patrepeval huggingface dataset:
Limited Protection from a Pathogenic Chimeric Simian-Human Immunodeficiency Virus Challenge following Immunization with Attenuated Simian Immunodeficiency Virus (1999)

ABSTRACT Two live attenuated single-deletion mutant simian immunodeficiency virus (SIV) constructs, SIV 239Δnef and SIV PBj6.6Δnef , were tested for their abilities to stimulate protective immunity in macaques. During the immunization period the animals were examined for specific immune responses and virus growth. Each construct generated high levels of specific immunity in all of the immunized animals. The SIV 239Δnef construct was found to grow to high levels in all immunized animals, with some animals remaining positive for virus isolation and plasma RNA throughout the immunization period. The SIV PBj6.6Δnef was effectively controlled by all of the immunized animals, with virus mostly isolated only during the first few months following immunization and plasma RNA never detected. Following an extended period of immunization of over 80 weeks, the animals were challenged with a pathogenic simian-human immunodeficiency virus (SHIV) isolate, SIV 89.6PD , by intravenous injection. All of the SIV 239Δnef -immunized animals became infected with the SHIV isolate; two of five animals eventually controlled the challenge and three of five animals, which failed to check the immunizing virus, progressed to disease state before the unvaccinated controls. One of five animals immunized with SIV PBj6.6Δnef totally resisted infection by the challenge virus, while three others limited its growth and the remaining animal became persistently infected and eventually died of a pulmonary thrombus. These data indicate that vaccination with attenuated SIV can protect macaques from disease and in some cases from infection by a divergent SHIV. However, if animals are unable to control the immunizing virus, potential damage that can accelerate the disease course of a pathogenic challenge virus may occur.
Kinetochore stretching inactivates the spindle assembly checkpoint (2009)

The spindle assembly checkpoint (SAC) monitors the attachment of microtubules to the kinetochore and inhibits anaphase when microtubule binding is incomplete. The SAC might also respond to tension; however, how cells can sense tension and whether its detection is important to satisfy the SAC remain controversial. We generated a HeLa cell line in which two components of the kinetochore, centromere protein A and Mis12, are labeled with green and red fluorophores, respectively. Live cell imaging of these cells reveals repetitive cycles of kinetochore extension and recoiling after biorientation. Under conditions in which kinetochore stretching is suppressed, cells fail to silence the SAC and enter anaphase after a delay, regardless of centromere stretching. Monitoring cyclin B levels as a readout for anaphase-promoting complex/cyclosome activity, we find that suppression of kinetochore stretching delays and decelerates cyclin B degradation. These observations suggest that the SAC monitors stretching of kinetochores rather than centromeres and that kinetochore stretching promotes silencing of the SAC signal.
Aging of the Skin (1997)

There are precious few benefits, save perhaps experience and wisdom, that those of advanced age may claim over those who continue to dwell in their youth. One somewhat paradoxical advantage, however, is the ability of older patients to apparently heal better than younger patients after cutaneous surgery. In older patients, the incision lines are less red, the scarring is less hypertrophic, and "normalization" of appearance occurs more rapidly. And yet, the "wrapping" does not necessarily reflect the contents of the "box." Unfavorable age-dependent alterations in the physical properties of the skin and the wound-healing cascade may affect the viability and structural integrity of the postoperative result. Surgery on the aged population must therefore couple the optimism for a pleasing aesthetic result with the caution reflecting a cutaneous substrate altered by the perturbations of time. This somewhat shaky balance, although not altogether understood, is worthy of study by the physician approaching the patient of advanced age. Arch Dermatol. 1997;133:1273-1277
Unplanned extubation in critically ill adults: clinical review (2012)

Identifying risk factors for UE and minimizing UE incidence through appropriate preventive strategies are prerequisites for improving nursing care quality and patient safety in the ICU.
Adult T-cell leukaemia/lymphoma (2007)

Adult T-cell leukaemia/lymphoma (ATLL) is a mature T-cell neoplasm of post-thymic lymphocytes aetiologically linked to the human T-cell lymphotropic virus, HTLV-I, and with a distinct geographical distribution. The disease manifests with leukaemia in greater than two thirds of patients, while the remaining patients have a lymphomatous form. According to the disease manifestations, various forms which differ in clinical course and prognosis have been recognised: acute, chronic, smouldering and lymphoma. Organomegaly, skin involvement, circulating atypical lymphocytes ("flower" cells) with a CD4+ CD25+ phenotype and hypercalcaemia are the most common disease features. The diagnosis should be based on a constellation of clinical features and laboratory investigations. The latter comprise: lymphocyte morphology, immunophenotype, histology of the tissues affected in the pure lymphoma forms and serology or DNA analysis for HTLV-I. The differential diagnosis of ATLL includes other mature T-cell neoplasms such as T-cell prolymphocytic leukaemia (T-PLL), Sézary syndrome (SS), peripheral T-cell lymphomas and occasionally healthy carriers of the virus or Hodgkin disease. The clinical course is aggressive with a median survival of less than 12 months in the acute and lymphoma forms. Despite major advances in understanding the pathogenesis of the disease, management of these patients remains a challenge for clinicians as they do not respond or achieve only transient responses to therapies used in high-grade lymphomas. The use of antiretroviral agents such as zidovudine in combination with interferon-alpha, with or without concomitant chemotherapy, has shown activity in this disease with improvement in survival and response rate. Consolidation with high dose therapy and autologous or allogeneic stem-cell transplantation should be considered in young patients.
Peripheral Blood Stem Cell Collection in Elderly Patients (2008)

Abstract Introduction Intensive treatments like autologous stem cells transplantations are final standard treatment in lymphoma and myeloma for young people (1,2). In the context of population’s ageing, those treatments should be eligible for older patients. One of the major causes which did not allowed these procedures in elderly patients is the reports of the impact of the age on the process of mobilisation of peripheral stem cell (3). We proceed a retrospective study to explore the feasibility of stem cells collection in patients older than 65 years, compared to younger people. Material and patients During the period of 1999–2006, we identified all the patients older than 65 years, with myeloma and lymphoma, who performed peripheral stem cells collection in Brest’s blood collection centre. We excluded patients for bone marrow collection. Patients were eligible if they achieved more than 2.106 CD34/kg. Then we compared the results obtained in older patients with the results obtained in younger people. Results We identified 100 patients older than 65 years between the 359 patients who underwent peripheral stem cell harvest in Brest’s harvest centre. We excluded 37 others patients for non-conformity. The elderly patients were significantly older (age 68.9 vs. 49.3 y, p&lt;10−6). No differences were seen for the number of collection between the older and the younger patients (1.59 vs. 1.50, p&gt;0.05). The number of CD34/kg per patient was sufficient to realise more than one autologous transplantation in both groups of patients (5.3 vs. 6.67, p=0.004). Samples were sufficiently rich for CD34/kg (4.36 vs. 5.54, p=0.02). Patients were harvested in one (60), two (25) or more than 2 collections (15). Among the older patients, 71% achieved a sufficient collection of more than 2.106 CD34/kg in one sample only. Conclusion We identified one hundred patients older than 65 years, during a period of 8 years, who performed peripheral stem cells collection, with sufficient wealth (&gt;2.106 CD34/kg). The majority of patients, 60%, need only one stem cells harvesting. The study confirmed that old patients could be harvested like young people, and eligible for stem cells autologous transplantation.
Phospholipids chiral at phosphorus. 2. Preparation, property, and application of chiral thiophospholipids (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhospholipids chiral at phosphorus. 2. Preparation, property, and application of chiral thiophospholipidsKarol Bruzik, Sanjeev M. Gupte, and Ming Daw TsaiCite this: J. Am. Chem. Soc. 1982, 104, 17, 4682–4684Publication Date (Print):August 1, 1982Publication History Published online1 May 2002Published inissue 1 August 1982https://pubs.acs.org/doi/10.1021/ja00381a033https://doi.org/10.1021/ja00381a033research-articleACS PublicationsRequest reuse permissionsArticle Views83Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Time-Domain Measurement System for UWB Microwave Imaging (2018)

A fully automatic time-domain measurement system for ultra-wideband (UWB) microwave imaging is presented. The system uses a pair of movable antennas to transmit and receive custom UWB pulse designs with each antenna able to be independently controlled to rotate about a region of interest. A full measurement, consisting of both incident and total field collections, is completed within 20 min using custom software. The collected data is processed to reconstruct images of three test objects. Imaging results demonstrate the system can localize and recognize different shaped objects, and differentiate multiple objects. The precision of antenna positioning and the impact of random noise on the microwave signal are investigated. The maximal positioning error for the antennas allows for accurate use of the system up to 7 GHz. A comparison between the time-domain system and a frequency-sweep method shows both methods allow for accurate imaging results. The signal-to-noise ratio and image quality may be improved with time-domain results when properly selecting a time window for the received signal.
Heterologous protein secretion by<i>Lactobacillus plantarum</i>using homologous signal peptides (2008)

The homologous signal peptides tested out, in this study, could be useful in food-grade systems for secretion of interesting proteins in Lact. plantarum. The constructed modular secretion vectors are easily accessible for rapid signal peptide screening.
HOPSMAN: An Experimental Testbed System for a 10-Gb/s Optical Packet-Switched WDM Metro Ring Network (2008)

For future WDM MANs, optical packet-switching has been considered to be a promising paradigm that efficiently supports a wide range of Internet-based applications having time-varying and high bandwidth demands and stringent delay requirements. This article presents the design of an experimental testbed system for a high-performance optical packet-switched WDM metro ring network, HOPSMAN. HOPSMAN boasts three crucial features. First, it has a scalable architecture in which the number of nodes is unconstrained by the number of wavelengths. Second, HOPSMAN nodes are equipped with high-speed photonic hardware components, including fast tunable receivers and optical slot erasers, capable of performing speedy optical packet-switching operations. Third, HOPSMAN incorporates a MAC scheme that embodies efficient and dynamic bandwidth allocation, resulting in exceptional delay-throughput performance. The article presents the key hardware components by highlighting the challenging issues we faced and the solutions we proposed for the testbed implementation. Finally, to demonstrate the feasibility of HOPSMAN, the article describes the experimental setup and presents the results obtained from running a commercially available remote media player application on the system.
An Improved Channel Estimation Technique for IEEE 802.11p Standard in Vehicular Communications (2018)

IEEE 802.11p based Dedicated Short-Range Communication (DSRC) is considered a potential wireless technology to enable transportation safety and traffic efficiency. A major challenge in the development of IEEE 802.11p technology is ensuring communication reliability in highly dynamic Vehicle-to-Vehicle (V2V) environments. The design of IEEE 802.11p does not have a sufficient number of training symbols in the time domain and pilot carriers in the frequency domain to enable accurate estimation of rapidly varying V2V channels. The channel estimation of IEEE 802.11p is preamble based, which cannot guarantee a suitable equalization in urban and highway scenarios, especially for longer length data packets. This limitation has been investigated by some research works, which suggest that one major challenge is determining an accurate means of updating channel estimate over the course of packet length while adhering to the standard. The motivation behind this article is to overcome this challenge. We have proposed an improved Constructed Data Pilot (iCDP) scheme which adheres to the standard and constructs data pilots by considering the correlation characteristics between adjacent data symbols in time domain and adjacent subcarriers in frequency domain. It is in contrast to previous schemes which considered the correlation in the time domain. The results have shown that the proposed scheme performs better than previous schemes in terms of bit error rate (BER) and root-mean-square error (RMSE).
112.8-Gb/s PM-RZ-64QAM optical signal generation and transmission on a 12.5GHz WDM grid (2010)

By employing a novel scheme we have generated 112.8-Gb/s polarization-multiplexed (PM)-RZ-64QAM optical signal. Transmission of 112.8-Gb/s PM-RZ-64QAM optical signal over 2×40km of SSMF has been demonstrated in an 8-channel WDM environment on a 12.5GHz grid.
Arrhythmia detection and classification using morphological and dynamic features of ECG signals (2010)

Computer-assisted cardiac arrhythmia detection and classification can play a significant role in the management of cardiac disorders. In this paper, we propose a new approach for arrhythmia classification based on a combination of morphological and dynamic features. Wavelet Transform (WT) and Independent Component Analysis (ICA) are applied separately to each heartbeat to extract corresponding coefficients, which are categorized as 'morphological' features. In addition, RR interval information is also obtained characterizing the 'rhythm' around the corresponding heartbeat providing 'dynamic' features. These two different types of features are then concatenated and Support Vector Machine (SVM) is utilized for the classification of heartbeats into 15 classes. The procedure is applied to the data from two ECG leads independently and the two results are fused for the final decision. Compare the two classification results and the classification result is kept if the two are identical or the one with greater classification confidence is picked up if the two are inconsistent. The proposed method was tested over the entire MIT-BIH Arrhythmias Database [1] and it yields an overall accuracy of 99.66% on 85945 heartbeats, better than any other published results.
Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line (2015)

Retinal ganglion cell (RGC) injury and cell death from glaucoma and other forms of optic nerve disease is a major cause of irreversible vision loss and blindness. Human pluripotent stem cell (hPSC)-derived RGCs could provide a source of cells for the development of novel therapeutic molecules as well as for potential cell-based therapies. In addition, such cells could provide insights into human RGC development, gene regulation, and neuronal biology. Here, we report a simple, adherent cell culture protocol for differentiation of hPSCs to RGCs using a CRISPR-engineered RGC fluorescent reporter stem cell line. Fluorescence-activated cell sorting of the differentiated cultures yields a highly purified population of cells that express a range of RGC-enriched markers and exhibit morphological and physiological properties typical of RGCs. Additionally, we demonstrate that aligned nanofiber matrices can be used to guide the axonal outgrowth of hPSC-derived RGCs for in vitro optic nerve-like modeling. Lastly, using this protocol we identified forskolin as a potent promoter of RGC differentiation.
Active Fusion Model with Robustness against Partial Occlusions (2006)

이동 물체 추적에 있어서 배경과 이동 물체의 동적인 변화는 폐색이라는 문제를 발생시키는 중요한 원인이다. 그리고 이러한 폐색이 발생하는 환경에서는 이동 물체 추적의 정확도가 현저하게 감소한다 따라서 본 논문에서는 배경 또는 다른 물체에 의해 발생하는 부분적 폐색에 강건한 활동적 퓨전 모델을 제안한다. 활동적 퓨전 모델은 이동 물체의 경계선 특징을 기반으로 하는 전통적인 기존의 스네이크 모델과 경계선 내부의 영역 특징을 고려하는 영역 기반 스네이크 모델로 구성된다. 이것은 먼저 이동 물체에 발생하는 부분적 폐색의 종류를 윤곽선 폐색과 영역폐색으로 구분한 뒤 폐색이 발생하는 위치와 폐색량에 따라서 각 모델의 신뢰도를 조절함으로써 부분적 폐색문제를 극복한다. 실험 결과에서는 부분적으로 폐색이 발생하는 환경에서 기본 방법들이 이동물체 추적에 실패하는 반면에 제안하는 방법은 추적에 성공함을 보인다. The dynamic change of background and moving objects is an important factor which causes the problem of occlusion in tracking moving objects. The tracking accuracy is also remarkably decreased in the presence of occlusion. We therefore propose an active fusion model which is robust against partial occlusions that are occurred by background and other objects. The active fusion model is consisted of contour-based md region-based snake. The former is a conventional snake model using contour features of a moving object and the latter is a regional snake model which considers region features inside its boundary. First, this model classifies total occlusion into contour and region occlusion. And then it adjusts the confidence of each model based on calculating the location and amount of occlusion, so it can overcome the problem of occlusion. Experimental results show that the proposed method can successfully track a moving object but the previous methods fail to track it under partial occlusion.
Antimicrobial Activity and Spectrum of Cefovecin, a New Extended- Spectrum Cephalosporin, against Pathogens Collected from Dogs and Cats in Europe and North America (2006)

Cefovecin is a new extended-spectrum semisynthetic cephalosporin indicated for the treatment of bacterial infections in dogs and cats. This study evaluated the in vitro activity and spectrum of cefovecin against 2,641 recent clinical isolates (1,660 canine and 981 feline isolates) from Europe and the United States. MIC determinations against cefovecin and other reference antimicrobials were performed by broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). Cefovecin demonstrated bactericidal activity against both gram-positive and gram-negative pathogens. Cefovecin exhibited in vitro activity against all major aerobic and anaerobic bacterial pathogens associated with skin, urinary tract, and periodontal infections in dogs and cats. The MIC90 values of cefovecin against Staphylococcus intermedius, Escherichia coli, and Pasteurella multocida were 0.25 microg/ml, 1.0 microg/ml, and 0.06 microg/ml, respectively. No significant differences were observed in terms of the activities of cefovecin against pathogens from different European countries and against pathogens of European and U.S. origin.
Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acid (1976)

A fluorescent base wye (Yt base) was isolated from Torulopsis utilis tRNAPhe. The structure was established as 4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazo[1,2-alpha]purine based on ultraviolet (uv), nuclear magnetic resonance (NMR), and mass spectra, and by direct comparison with synthetic material. The nucleoside, wyosine (Yt), was isolated from purified tRNAPhe by enzymatic degradations followed by column and thin-layer chromatographies. The structure of wyosine is proposed as 3-ribofuranosyl-4,9-dihydro-4,6-dimethyl-9-oxo-1H-imidazol[1,2-alpha]purine (the ribosyl group is attached to the N-9 position of guanine nucleus) on the basis of comparison with model compounds.
Wireless Communication Role in Patient Response Time (2008)

This study investigated the use and impact of wireless communication technology developed by Vocera Communications and implemented at St Agnes Hospital, Baltimore, MD. The specific focus was on the impact of a newly installed component of the Vocera system, the Vocera Messaging Interface, which enables connectivity between third-party systems, such as a nurse call system. The results of the investigation of the nurse call integration confirmed that the use of the integrated communications system reduced overall mean time for completing a patient request by 51% across all observations when controlling for observation type. Furthermore, analysis of clinicians' usage of the system for different types of patient requests revealed that it enables the clinician to have more control in prioritizing and responding to requests according to the seriousness of the event. The study also exposed several "creative" and "evolving" impacts of the system that are discussed along with practical implications of the findings.
Developments and applications of cyanoacrylate adhesives (1989)

—Various developments in the synthesis of alkyl 2-cyanoacrylates, their analytical and test methods, adhesive compositions for different applications including those in the medical field, handling and storage, and health and safety hazards are reviewed.
Production and characterization of transgenic rice plants carrying a high-affinity nitrate transporter gene (OsNRT2.1) (2009)

Enhancing nitrogen utilization is important in maintaining crop yield under low nitrogen conditions. Therefore, rice plants (Oryza sativa L.) were transformed with pMLH7133-RNRT2, which bears OsNRT2.1, a major gene involved in nitrate uptake under low nitrate concentrations. Two transgenic plants harboring an OsNRT2.1 transgene were identified and used for further study. In transgenic plants, OsNRT2.1 was expressed constitutively in roots, while in wild-type plants, the transcript was detected 30 min after starting to supply nitrate. No co-suppression between transgene and host gene was observed, as was observed on introduction of a nitrate reductase gene from the same species. One of the transformants grew faster than the wild-type cultivars when KNO3 or NH4NO3 was supplied as a nitrogen source. However, no increase in nitrate uptake by young plants was observed. These results suggest that constitutive expression of OsNRT2.1 might enhance vegetative growth and that introduction of genes involved in nitrate uptake from the same species may be useful for genetic improvement of plant growth under low nitrogen conditions. Further studies are necessary to understand the molecular and physiological bases of expression of NRT2.1 in relation to plant growth.