gebhart/patrepeval huggingface dataset:
Effects of nitric oxide synthase inhibitor on hemodynamic change and O2 delivery in septic dogs (1995)

To elucidate the role of nitric oxide (NO) in septic shock, we measured hemodynamic and pulmonary gas changes in anesthetized dogs after intravenous administration of bacterial lipopolysaccharide (LPS) with or without NO synthase inhibitor, NG-nitro-L-arginine (L-NNA). Infusion of LPS (250 ng.kg-1.min-1) for 2 h decreased mean arterial pressure over 1-4 h. Although L-NNA (10 mg/kg) blocked LPS-induced hypotension, it decreased cardiac index, oxygen delivery index, arterial pH, and arterial PO2 and increased systemic vascular resistance index in the presence or absence of LPS. Administration of NG-nitro-D-arginine (D-NNA, 10 mg/kg) alone caused fewer hemodynamic effects (increased systemic vascular resistance index and decreased cardiac index) than L-NNA alone. Our study provides evidence that L-NNA prevents endotoxin-induced hypotension but decreases cardiac output and oxygen delivery, effects that may, in part, be due to a nonspecific NO synthase-independent event. Thus clinical use of NO synthase inhibitors for the treatment of septic shock should be cautiously considered.
Sensing damage by the NLRP3 inflammasome (2011)

The NLRP3 inflammasome is activated in response to a variety of signals that are indicative of damage to the host including tissue damage, metabolic stress, and infection. Upon activation, the NLRP3 inflammasome serves as a platform for activation of the cysteine protease caspase-1, which leads to the processing and secretion of the proinflammatory cytokines interleukin-1β (IL-1β) and IL-18. Dysregulated NLRP3 inflammasome activation is associated with both heritable and acquired inflammatory diseases. Here, we review new insights into the mechanism of NLRP3 inflammasome activation and its role in disease pathogenesis.
Deletion of the Mint3/Apba3 Gene in Mice Abrogates Macrophage Functions and Increases Resistance to Lipopolysaccharide-induced Septic Shock (2011)

Two major metabolic systems are usually used to generate ATP: oxidative phosphorylation (OXPHOS) in the mitochondria and glycolysis. Most types of cells employ OXPHOS for ATP production during normoxia but then shift energy production from OXPHOS to glycolysis when exposed to hypoxia. Hypoxia-inducible factor-1 (HIF-1) is the master transcription factor regulating this metabolic shift. On the other hand, macrophages are unique in making use of glycolysis for ATP generation constitutively even during normoxia. We recently proposed that in macrophages, Mint3/APBA3 inhibits factor inhibiting HIF-1 (FIH-1) during normoxia, which in turn releases the suppression of HIF-1 activity by FIH-1. To demonstrate the physiological function of APBA3 in macrophages, we established Apba3(-/-) mice. The mutant mice presented no apparent gross phenotype but exhibited significant resistance against LPS-induced septic shock. The level of ATP in macrophages obtained from the mutant mice was reduced to 60% of the level observed in wild type cells, which in turn led to reduced ATP-dependent activities such as glycolysis, cytokine production, and motility. We also generated mutant mice with the Apba3 gene deleted specifically from cells of the myeloid lineage and confirmed that LPS-induced septic shock is mitigated significantly. Thus, we show cell type-specific regulation of energy production by APBA3 in macrophages using genetically manipulated mice. The specific function of APBA3 in macrophages might allow us to develop therapeutics to regulate aberrant macrophage function during infection and diseases.
High‐speed aqueous gel‐permeation chromatography (1978)

Abstract High‐speed gel‐permeation chromatography (GPC) of water‐soluble polymers has been investigated by using TSK‐GEL, type‐PW columns packed with small porous particles. Being semirigid, the column packing could be operated under high pressure and, therefore, it was possible to achieve high‐speed GPC. A resolution higher than that of ordinary GPC in organic solvent system was attained when the measurement of one sample was completed within 40 min with a 6‐ft column set. Samples with a wide range of molecular weights, from oligomers to polymers having a molecular weight greater than 10 6 , could be fractionated. Dextran, polyethylene glycol, polyacrylamide, poly(vinyl alcohol), and poly(vinyl pyrrolidone) were separated according to molecular size with no evidence of adsorption. Investigation of other water soluble polymers is now in progress.
Molecular Analysis of Human Prorenin Prosegment Variants in Vitro and in Vivo (1995)

The aspartyl protease renin, an important modulator of blood pressure in humans, is present in the circulation not only in its active form, but also as an inactive precursor, prorenin, in which a 43-amino acid prosegment blocks access of the substrate to the active site of the enzyme. Site-directed mutagenesis of the prosegment has led to the following conclusions. 1) Maintenance of the enzymatically inactive state of prorenin requires a short peptide sequence between positions 10P and 20P (where P denotes prosegment and numbering is relative to amino terminus) of the prosegment; and 2) there is an inverse relationship between the ability of prosegment mutations to activate and their effect on the secretion of the various prorenins, suggesting that this same region of the prosegment plays a critical role in the biosynthesis of human prorenin. Since these results demonstrated that single amino acid mutations could activate human prorenin to varying degrees, mutations in this region of the renin gene could be clinically important in humans. To test this hypothesis, genomic screening was carried out on the corresponding region of the human renin gene (exon 2) in a cohort of patients selected for a likely familial component to their hypertension. While this study identified a novel polymorphism in exon 2 of the human renin gene, evidence was not obtained for either the presence of prosegment mutations or the association of the novel polymorphism with hypertension in the patient population studied. In conclusion, both structure-function studies and genetic screening suggest that mutation of the prorenin prosegment is an unlikely factor in activation of the renin-angiotensin system in humans.
Testosterone Signaling through Internalizable Surface Receptors in Androgen Receptor-free Macrophages (1999)

Testosterone acts on cells through intracellular transcription-regulating androgen receptors (ARs). Here, we show that mouse IC-21 macrophages lack the classical AR yet exhibit specific nongenomic responses to testosterone. These manifest themselves as testosterone-induced rapid increase in intracellular free [Ca(2+)], which is due to release of Ca(2+) from intracellular Ca(2+) stores. This Ca(2+) mobilization is also inducible by plasma membrane-impermeable testosterone-BSA. It is not affected by the AR blockers cyproterone and flutamide, whereas it is completely inhibited by the phospholipase C inhibitor U-73122 and pertussis toxin. Binding sites for testosterone are detectable on the surface of intact IC-21 cells, which become selectively internalized independent on caveolae and clathrin-coated vesicles upon agonist stimulation. Internalization is dependent on temperature, ATP, cytoskeletal elements, phospholipase C, and G-proteins. Collectively, our data provide evidence for the existence of G-protein-coupled, agonist-sequestrable receptors for testosterone in plasma membranes, which initiate a transcription-independent signaling pathway of testosterone.
Resettlement policy framework and procedural guidelines (2001)

This policy framework provides the principles, and provisions to compensate the population negatively affected by the Rural Electrification, and, the Renewable Energy Development projects, and, is based on the Bank's operational directives regarding involuntary resettlement, and on Cambodian law. Measures to minimize impacts on the project affected families (PAFs), focus primarily on a careful design, and selection of the proposed site distribution, so as to reduce land acquisition needs, and help improve, or at least maintain the PAF's living standards. Housing, and structures affected will be compensated at replacement costs, while land losses will be at land-for-land of equal agricultural productive capacity. However, where land is not available, compensation should be provided in cash at replacement costs, based on market values. During the resettlement transitional period, subsidies - or living allowances - will be available for relocation, and moving expenses. Business, crop, and/or miscellaneous production losses will be compensated at replacement costs, and, in seeking to improve living conditions, the provision of training assistance will enhance skills, access to credit will improve income capacity, and, the implementation of the project itself, should create new job opportunities. Grievance mechanisms, and a monitoring criteria will ensure the appropriate resettlement process, as per consultations with PAFs.
Identification of 1,4-dihydropyridine binding regions within the alpha 1 subunit of skeletal muscle Ca2+ channels by photoaffinity labeling with diazipine. (1991)

To identify regions that are involved in the formation of the dihydropyridine receptor site of skeletal muscle L-type Ca2+ channels, the alpha 1 subunit of the channel complex was specifically labeled with the 1,4-dihydropyridine-receptor-selective photoaffinity probe [3H]diazipine. Photoaffinity-labeled regions were identified by probing labeled proteolytic fragments with several anti-peptide antibodies recognizing different segments of the alpha 1 sequence. Forty to 50% of the alpha 1-associated [3H]diazipine label was contained in the tryptic fragment between Arg-988 and Ala-1023 derived from the loop between segments S5 and S6 in domain III. This region corresponds to a portion of the channel that is believed to contribute to formation of the transmembrane pore. Twenty to 30% of the labeling occurred in a V8 protease fragment between Glu-1349 and Trp-1391. This fragment contains transmembrane segment S6 of domain IV and has previously been shown to form part of the drug receptor for phenylalkylamine Ca2+ antagonists. Our data suggest that the dihydropyridine receptor is formed by close apposition of two discontinuous regions of the alpha 1 subunit sequence in domains III and IV. In light of previous work localizing this receptor site to the extracellular surface of the lipid bilayer, it is proposed that amino acid residues at the extracellular surface in the loop connecting segments IIIS5 and IIIS6 and at the extracellular end of segment IVS6 contribute to formation of the dihydropyridine receptor site.
Structural analysis of La<sub>2</sub>Mo<sub>2</sub>O<sub>9</sub>-based fast oxide-ion conductors (2005)

The most important unsolved problem for the introduction of the Hydrogen Economy is efficient and safe storage of hydrogen. Alanates, compounds based on the AlH 4 -unit, are among the most promising metal hydrides for reversible hydrogen storage. The storage capacity is large, e.g. NaAlH 4 can release 5.6 wt% hydrogen below 200 C. Work during the last years has revealed that Ti additives improve the kinetics of NaAlH 4 and also make re-hydrogenation possible. In order to improve the understanding of the effect of additives and absorption/desorption processes in general, detailed structural studies are very important.
Active sonic boom control (1996)

A theory and simulation code are developed to study non-steady sources as means to control sonic booms of supersonic aircraft. A key result is that the source of sonic boom pressure is not confined to the length of the aircraft but occupies an extensive segment of the flight path. An aircraft in non-steady flight functions as a synthetic aperture antenna, generating complex acoustic waves with no simple relation to instantaneous volume or lift distributions. The theory applies linear acoustics to slender non-steady sources but requires no far-field approximation. The solution for pressure contains a term not seen in Whitham's theory for sonic booms of distant supersonic aircraft. The term describes a pressure field that decays algebraically behind the Mach cone and, in the case of steady flight, integrates to a ground load equal to the weight of the aircraft. The algebraic term is separate from those that describe the sonic boom. Two non-steady source phenomena are evaluated: periodic velocity changes (surge), and periodic longitudinal lift redistribution (slosh). Surge can attenuate a sonic boom and covert it into prolonged weak reverberation, but accelerations needed to produce the phenomenon seem too large for practical use. Slosh may be practical and can alter sonic booms but does not, on average, result in boom attenuation. The conclusion is that active sonic boom abatement is possible in theory but maybe not practical.
Increasing frequencies of site-specific mutagenesis and gene targeting in <i>Arabidopsis</i> by manipulating DNA repair pathways (2013)

Improved methods for engineering sequence-specific nucleases, including zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs), have made it possible to precisely modify plant genomes. However, the success of genome modification is largely dependent on the intrinsic activity of the engineered nucleases. In this study, we sought to enhance ZFN-mediated targeted mutagenesis and gene targeting (GT) in Arabidopsis by manipulating DNA repair pathways. Using a ZFN that creates a double-strand break (DSB) at the endogenous ADH1 locus, we analyzed repair outcomes in the absence of DNA repair proteins such as KU70 and LIG4 (both involved in classic nonhomologous end-joining, NHEJ) and SMC6B (involved in sister-chromatid-based homologous recombination, HR). We achieved a fivefold to 16-fold enhancement in HR-based GT in a ku70 mutant and a threefold to fourfold enhancement in GT in the lig4 mutant. Although the NHEJ mutagenesis frequency was not significantly changed in ku70 or lig4, DNA repair was shifted to microhomology-dependent alternative NHEJ. As a result, mutations in both ku70 and lig4 were predominantly large deletions, which facilitates easy screening for mutations by PCR. Interestingly, NHEJ mutagenesis and GT at the ADH1 locus were enhanced by sixfold to eightfold and threefold to fourfold, respectively, in a smc6b mutant. The increase in NHEJ-mediated mutagenesis by loss of SMC6B was further confirmed using ZFNs that target two other Arabidopsis genes, namely, TT4 and MPK8. Considering that components of DNA repair pathways are highly conserved across species, mutations in DNA repair genes likely provide a universal strategy for harnessing repair pathways to achieve desired targeted genome modifications.
Clinical Aspects of Clara Cell 10-kDa Protein / Uteroglobin (Secretoglobin 1A1) (2003)

Clara cell 10-kDa protein (CC10)/ uteroglobin (UG) is a nonglycoprotein with a molecular mass of 16 kilodaltons, which is produced by mucosal epithelial cells in the lung (Clara cells), uterus and prostate. Like other low molecular weigh proteins it is catabolized in renal proximal tubules. Structurally it is a homodimer of subunits of 70 amino acids covalently bound in an antiparallel manner. It belongs to secretogobin (SCGB) family and is assigned as subgroup 1A1. The function of the protein so far elucidated is immunoregulatory and anti-inflammatory in innate immunity. The knockout mouse of UG gene resulted in aggravation of inflammation by allergic and hyperoxic stimuli. It also showed very similar pathological features with human IgA nephropathy. The value is changed in the lung fluid and serum of various inflammatory and allergic lung diseases. Several kinds of single nucleotide polymorphisms (SNPs) in human CC10/UG gene were recently discovered; Adenine allele accumulation in G38A SNP has possible association with asthma and IgA nephropathy, being paralleled with disease severity of IgA nephropathy. Its expression is enhanced by some transcriptional factors induced by cytokines such as interferon-gamma. For cancer cells, the protein functions as an antagonist of neoplastic phenotype. CC10/UG forms one of intra- and intercellular regulators involved in inflammation and malignant transformation in the respiratory and urogenital fields.
Molecular Representations of Ratawi and Alaska North Slope Asphaltenes Based on Liquid- and Solid-State NMR (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular Representations of Ratawi and Alaska North Slope Asphaltenes Based on Liquid- and Solid-State NMRD. A. Storm, J. C. Edwards, S. J. DeCanio, and E. Y. SheuCite this: Energy Fuels 1994, 8, 3, 561–566Publication Date (Print):May 1, 1994Publication History Published online1 May 2002Published inissue 1 May 1994https://pubs.acs.org/doi/10.1021/ef00045a007https://doi.org/10.1021/ef00045a007research-articleACS PublicationsRequest reuse permissionsArticle Views328Altmetric-Citations71LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Cholesteryl Pullulan Encapsulated TNF-<i>α</i>Nanoparticles Are an Effective Mucosal Vaccine Adjuvant against Influenza Virus (2015)

We encapsulated tumor necrosis factor-α (TNF-α), a major proinflammatory cytokine, into cholesteryl pullulan (CHP) to prepare TNF/CHP nanoparticles. In this report, we describe the immune-enhancing capability of the nanoparticles to act as a vaccine adjuvant. TNF/CHP nanoparticles showed excellent storage stability and enhanced host immune responses to external immunogens. The nanoparticles were effective via the nasal route of administration for inducing systemic IgG1 as well as mucosal IgA. We applied the nanoparticles in a model experimental influenza virus infection to investigate their adjuvant ability. TNF/CHP nanoparticles combined with a conventional split vaccine protected mice via nasal administration against a lethal challenge of A/PR/8/34 (H1N1) influenza virus. Mechanistic studies showed that the nanoparticles enhanced antigen uptake by dendritic cells (DCs) and moderately induced the expression of inflammation-related genes in nasopharynx lymphoid tissue (NALT), leading to the activation of both B and T cells. Preliminary safety study revealed no severe toxicity to TNF/CHP nanoparticles. Slight-to-moderate influences in nasal mucosa were observed only in the repeated administration and they seemed to be reversible. Our data show that TNF/CHP nanoparticles effectively enhance both humoral and cellular immunity and could be a potential adjuvant for vaccines against infectious diseases, especially in the mucosa.
A Vogad for Radiotelephone Circuits (1939)

Commercial radiotelephone connections must generally be accessible to any telephone in an extensive wire system. Speech signals delivered to the radio terminals for transmission to distant points vary widely in amplitude due to the characteristics of the wire circuits and individual voices. To provide the best margin against atmospheric noise, it is usually the practice to equalize this wide range of speech amplitudes and thus drive the radiotelephone transmitter at its full capacity. Many devices have been proposed to adjust automatically the gain in a circuit to equalize speech volumes. The difficulties of providing a device which will respond properly over a wide range to the complex qualities of a speech signal have only recently been overcome to a satisfactory degree. The voice-operated gain-adjusting device, or "vogad," described in this paper is a practical design based upon more than a year's experience with one of the most promising devices made available by earlier development effort. A trial installation of this latest vogad is now under way at Norfolk in connection with a new radiotelephone system for harbor and coastal service.
Molecular biology of GABA <sub>A</sub> receptors (1990)

The major type of receptor for the inhibitory neurotransmitter gamma-aminobutyric acid (GABA), called the GABAA receptor, is a member of a gene superfamily of ligand-gated ion channels. This receptor is a hetero-oligomeric protein composed of several distinct polypeptide types (alpha, beta, gamma, and delta). Molecular cloning of these polypeptides reveals that they show 20-40% identity with each other, and 10-20% identity with polypeptides of the nicotinic acetylcholine receptors and strychnine-sensitive glycine receptor. Each polypeptide type is also represented by a family of genes whose members have 60-80% amino acid sequence identity. Regions of conserved and variable amino acid sequence suggest structural and functional domains within each polypeptide. All of the polypeptides when expressed in heterologous cells produce GABA-activated chloride channels, and the different subtypes express different pharmacological properties. The distributions of mRNAs for the different GABAA receptor polypeptides and their subtypes show significant brain regional variation consistent with pharmacological and biochemical evidence for receptor heterogeneity. Subpopulations of GABAA receptors with different cellular and regional locations show differential sensitivity to GABA, to modulators like steroids, to physiological regulation, to disease processes, and to pharmacological manipulation by drugs such as benzodiazepines. The properties of the different subpopulations of GABAA receptors are determined by which one or more of the different polypeptides and their subtypes are expressed in a given cell to produce a variety of different oligomeric protein structures. Molecular cloning techniques have produced rapid advances in understanding the GABAA receptor protein family.
Fabrication and Structural Characterization of Large-Scale Uniform SnO<sub>2</sub> Nanowire Array Embedded in Anodic Alumina Membrane (2001)

Semiconductor SnO2 nanowire arrays were fabricated by electrochemical deposition and thermal oxidizing methods based on highly ordered nanoporous alumina membrane. Their microstructures were characterized by X-ray diffraction, transmission electron microscopy, Raman spectrum, and scanning electron microscopy. The results indicate that the SnO2 nanowire array with cassiterite polycrystalline structure is uniformly assembled into the hexagonally ordered nanochannels of anodic alumina membranes. There are three phases (Sn, SnO, and SnO2) coexisting when the as-deposited assembly system is annealed at 823 K. However, only the SnO2 cassiterite phase is detected when the assembly system is annealed at 923 K.
Ideas lingüísticas de Bernardo de Aldrete (1968)

No disponible.
Improvement of microstrip patch antenna radiation patterns (1994)

Microstrip patch antennas typically have radiation patterns containing unwanted sidelobes or local minima caused by surface waves, especially when fabricated on high dielectric-constant substrates. The paper shows that removal of the substrate beneath the patch can greatly improve the pattern. A variation of this technique, compatible with monolithic fabrication, is applied to fabrication of a patch on GaAs and shows similar pattern improvement.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>