gebhart/patrepeval huggingface dataset:
Na <sup>+</sup> -Ca <sup>2+</sup> Exchange Activity Is Localized in the T-Tubules of Rat Ventricular Myocytes (2002)

Detubulation of rat ventricular myocytes has been used to investigate the role of the t-tubules in Ca2+ cycling during excitation-contraction coupling in rat ventricular myocytes. Ca2+ was monitored using fluo-3 and confocal microscopy. In control myocytes, electrical stimulation caused a spatially uniform increase in intracellular [Ca2+] across the cell width. After detubulation, [Ca2+] rose initially at the cell periphery and then propagated into the center of the cell. Application of caffeine to control myocytes resulted in a rapid and uniform increase of intracellular [Ca2+]; the distribution and amplitude of this increase was the same in detubulated myocytes, although its decline was slower. On application of caffeine to control cells, there was a large, rapid, and transient rise in extracellular [Ca2+] as Ca2+ was extruded from the cell; this rise was significantly smaller in detubulated cells, and the remaining increase was blocked by the sarcolemmal Ca2+ ATPase inhibitor carboxyeosin. The treatment used to produce detubulation had no significant effect on Ca2+ efflux in atrial cells, which lack t-tubules. Detubulation of ventricular myocytes also resulted in loss of Na+-Ca2+ exchange current, although the density of the fast Na+ current was unaltered. It is concluded that Na+-Ca2+ exchange function, and hence Ca2+ efflux by this mechanism, is concentrated in the t-tubules, and that the concentration of Ca2+ flux pathways in the t-tubules is important in producing a uniform increase in intracellular Ca2+ on stimulation.
Shallow donors in multiple-well GaAs-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Ga</mml:mi></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mi mathvariant="normal">−</mml:mi><mml:mi mathvariant="normal">x</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Al</mml:mi></mml:mrow><mml:mrow><mml:mi mathvariant="normal">x</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math>As heterostructures (1986)

The effects of finite-width barriers upon the binding energies and probability distributions of shallow donor states in GaAs-${\mathrm{Ga}}_{1\mathrm{\ensuremath{-}}\mathrm{x}}$${\mathrm{Al}}_{\mathrm{x}}$As heterostructures are reported. We have performed calculations for the ground and low-lying excited states for donor positions within a GaAs well or a ${\mathrm{Ga}}_{1\mathrm{\ensuremath{-}}\mathrm{x}}$${\mathrm{Al}}_{\mathrm{x}}$As barrier. Although the binding energy of the ground state of a donor located at the center of a well is typically not strongly affected by barriers wider than an effective Bohr radius, those of the excited states are reduced significantly for the on-center case. All states considered show significant effects due to the neighboring wells if the donor is located near the edge of a well or within a barrier.
A Multi-Code Biorthogonal Code Keying with Constant Amplitude Coding (2005)

Orthogonal modulation provides low probability of bit error, however its bandwidth efficiency is very low. Biorthogonal code may double the bandwidth efficiency, but its required bandwidth grows exponentially with the number of input bits as in orthogonal modulation. In this paper, we propose a multi-code biorthogonal code keying (MBCK) scheme that significantly reduces the signal bandwidth with the benefit from orthogonal waveform coding maintained. The system consists of multiple waveform coding blocks, and the sum of output codewords is transmitted. A problem with MBCK is that output signal is multi-level, which requires amplifier with high linearity. So it may not be an appropriate scheme for portable unit where power efficiency is highly important. We also propose a modified MBCK scheme that guarantees constant amplitude output. The transmitter of the proposed scheme contains a redundant waveform coder whose input is generated by encoding the information bits. Adding the codewords from all constituent waveform coding blocks, the composite signal has constant amplitude. It is also shown that the redundant bits are not only used to make constant amplitude signal but also used to improve the BER performance at the receiver.
Activated Ion Electron Capture Dissociation for Mass Spectral Sequencing of Larger (42 kDa) Proteins (2000)

In previous studies, electron capture dissociation (ECD) has been successful only with ionized smaller proteins, cleaving between 33 of the 153 amino acid pairs of a 17 kDa protein. This has been increased to 99 cleavages by colliding the ions with a background gas while subjecting them to electron capture. Presumably this ion activation breaks intramolecular noncovalent bonds of the ion's secondary and tertiary structure that otherwise prevent separation of the products from the nonergodic ECD cleavage of a backbone covalent bond. In comparison to collisionally activated dissociation, this "activated ion" (AI) ECD provides more extensive, and complementary, sequence information. AI ECD effected cleavage of 116, 60, and 47, respectively, backbone bonds in 29, 30, and 42 kDa proteins to provide extensive contiguous sequence information on both termini; AI conditions are being sought to denature the center portion of these large ions. This accurate "sequence tag" information could potentially identify individual proteins in mixtures at far lower sample levels than methods requiring prior proteolysis.
Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp (1987)

Comparison of partial 16S rRNA sequences from representative Campylobacter species indicates that the Campylobacter species form a previously undescribed basic eubacterial group, which is related to the other major groups only by very deep branching. This analysis was extended to include the spiral bacterium associated with human gastritis, Campylobacter pylori (formerly Campylobacter pyloridis). The distance between C. pylori and the other Campylobacter species is sufficient to exclude the pyloric organism from the Campylobacter genus. The results indicate that C. pylori is more closely related to Wolinella succinogenes than it is to the other Campylobacter species inspected. Another close relative of the campylobacters was found to be Thiovulum, a sulfide-dependent marine bacterium.
La consommation des jus de fruits en France en 1970 (1971)

Comparaison (tableaux) de la commercialisation francaise 1969, 1970, 1971 de jus de fruits et nectars. Importations. Exportations. Consommation
SpinybotII: climbing hard walls with compliant microspines (2006)

A new climbing robot has been developed that can scale flat, hard vertical surfaces including concrete, brick, stucco and masonry without using suction or adhesives. The robot can carry a payload equal to its own weight and can cling without consuming power. It employs arrays of miniature spines that catch opportunistically on surface asperities. The approach is inspired by the mechanisms observed in some climbing insects and spiders. This paper covers the analysis and implementation of the approach, focusing on issues of spine/surface interaction and compliant suspension design
Effects of several putative beta 3-adrenoceptor agonists on lipolysis in human omental adipocytes. (1996)

None of the beta 3-adrenoceptor agonists tested is an ideal drug for therapeutic use in man (i.e. regarded as a selective and full agonist with high receptor potency). Only CL 316 243 may have a potential therapeutic role, although the potency is very low. CGP 12177 is useful as a reference substance for human in vitro studies.
HDAC inhibitors block innate immunity (2011)

In this issue of Blood, Roger and colleagues present data on the magnitude of influence that broad-spectrum HDAC inhibitors exert on TLR-driven immune responses, thus demonstrating that HDAC inhibitors are immunosuppressive drugs.
Magnetoresistance in magnetic manganese oxide with intrinsic antiferromagnetic spin structure (1993)

Giant and isotropic magnetoresistance as huge as −53% was observed in magnetic manganese oxide La0.72Ca0.25MnOz films with an intrinsic antiferromagnetic spin structure. We ascribe this magnetoresistance to spin-dependent electron scattering due to spin canting of the manganese oxide.
Software-Based Self Test Methodology for On-Line Testing of L1 Caches in Multithreaded Multicore Architectures (2012)

The flexibility that allows the application of different March tests is a critical requirement for on-line testing of memory arrays. In a previous study, we have introduced a low-cost software-based self test (SBST) program development methodology for on-line periodic testing of L1 caches that utilizes direct cache access (DCA) instructions and exploits the native monitoring hardware available in modern architectures. In this brief, we discuss a multithreaded optimization of this SBST methodology that exploits the thread level parallelism of multithreaded multicore architectures in order to speed up March test execution by elaborating the low level multiple sub-bank cache organization. The effectiveness of the methodology and its multithreaded optimization is demonstrated on the L1 caches of OpenSPARC T1 processor. Our results showed a speedup of more than 1.7 when the multithreaded optimization is applied and an acceptable performance overhead (less than 11%), even in intensive periodic test scenarios.
Ex Vivo Expansion of CD34+ Umbilical Cord Blood Cells in a Defined Serum-Free Medium (QBSF-60) with Early Effect Cytokines (1999)

To investigate the clinically applicable conditions that support substantial expansion of both primitive and more mature hematopoietic cells of umbilical cord blood (UCB) for transplantation in adults, enriched CD34+ cells from 8 fresh UCB samples and 4 expanded UCB products were cultured in defined serum-free medium (QBSF-60) in the presence of a cytokine combination of SCF, Flt-3-ligand (FL), thrombopoietin (TPO), IL-3 for up to 2 weeks. Fresh medium with cytokines was supplemented or exchanged at day 4, day 7, and day 10. The proliferative response was assessed at day 7, day 10, and day 14 by evaluating the following parameters: nucleated cell (NC), clonogenic progenitors (colony-forming unit-granulocyte-macrophage [CFU-GM], burst-forming unit-erythrocyte [BFU-E], CFU-GEMM, and high-proliferative potential colony-forming cell [HPP-CFC]), immunophenotypes (CD34+ cells and CD34+ subpopulations), and LTCIC. Simultaneously numerical expansion of various stem/progenitor cells, including primitive CD34+CD38-HLA-DR- subpopulation and LTCIC, CD34+ cells, and clonogenic progenitors to mature nucleated cells, were continuously observed during the culture. An average 103.32 +/- 71.37 x 10(6) CD34+ cells (range 10.12 x 10(6)-317.9 x 10(6)) could be obtained from initial 1.72 +/- 1.13 x 10(6) UCB CD34+ cells after 10-14 days cultured under the described conditions. Sufficient CD34+ cells (>50.0 x 10(6)) for transplantation in adults would be available in all but one UCB collections after 10-14 days expansion. The expanded CD34+ cells sustained most of the in vitro characteristics of initial unmanipulated CD34+ cells, including clonogenic efficiency (of both primitive and committed progenitors), the proportion of CD34+CD38-HLA-DR- subpopulation, and the expansion potential. Initial addition of IL-3 to the cocktail of SCF + FL + TPO had positive effects on the expansion of both primitive and, especially, the more mature hematopoietic cells. It accelerated the expansion speed and shortened the optimal culture time from 14 days to 10 days. These results indicated that our proposed short-term culture system, consisting of QBSF-60 serum-free medium with a simple early acting cytokine combination of SCF + FL + TPO, could substantially support simultaneous expansion of various stem/progenitor cell populations involved in the different phases of engraftment. It would be a clinically applicable protocol for ex vivo expansion of CD34+ UCB cells.
Computationally efficient E-model improvement of MOS estimate including jitter and buffer losses (2012)

In the article we propose a method of improving ITU-T E-model MOS estimate of VoIP call quality. The improvement consists of including the effects of network jitter as measured and distributed in RTCP packets; jitter buffer size at receiver and codec packetization settings as input parameters of E-Model. Our method uses Pareto/D/1/k system for modeling general VoIP input traffic stream interarrival times and jitter buffer behavior resulting in additional packet loss. Consequently we propose the inclusion of jitter buffer size, codec packetization and network jitter into E-Model by means of substitution of existing Ppl (packet loss) parameter for Pplef (effective packet loss) in computationally effective manner suitable for real-time MOS estimate without the need of capturing large packet traces from the network and performing estimation of Hurst parameter or using other means of traffic statistics description in advance.
Influence of electrospun fiber mesh size on hMSC oxygen metabolism in 3D collagen matrices: Experimental and theoretical evidences (2011)

The traditional paradigm of tissue engineering of regenerating in vitro tissue or organs, through the combination of an artificial matrix and a cellular population has progressively changed direction. The most recent concept is the realization of a fully functional biohybrid, where both, the artificial and the biotic phase, concur in the formation of the novel organic matter. In this direction, interest is growing in approaches taking advantage of the control at micro- and nano-scale of cell material interaction based on the realization of elementary tassels of cells and materials which constitute the beginning point for the expansion of 3D more complex structures. Since a spontaneous assembly of all these components is expected, however, it becomes more fundamental than ever to define the features influencing cellular behavior, either they were material functional properties, or material architecture. In this work, it has been investigated the direct effect of electrospun fiber sizes on oxygen metabolism of h-MSC cells, when any other culture parameter was kept constant. To this aim, thin PCL electrospun membranes, with micro- and nano-scale texturing, were layered between two collagen slices up to create a sandwich structure (µC-PCL-C and nC-PCL-C). Cells were seeded on membranes, and the oxygen consumption was determined by a phosphorescence quenching technique. Results indicate a strong effect of the architecture of scaffolds on cell metabolism, also revealed by the increasing of HIF1-α gene expression in nC-PCL-C. These findings offer new insights into the role of materials in specific cell activities, also implying the existence of very interesting criteria for the control of tissue growth through the tuning of scaffold architecture.
Modeling of Fluid Flow and Heat Transfer for Optimization of Pin-Fin Heat Sinks (2004)

I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final versions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public.
Higher-order functional reactive programming in bounded space (2012)

Functional reactive programming (FRP) is an elegant and successful approach to programming reactive systems declaratively. The high levels of abstraction and expressivity that make FRP attractive as a programming model do, however, often lead to programs whose resource usage is excessive and hard to predict. In this paper, we address the problem of space leaks in discrete-time functional reactive programs. We present a functional reactive programming language that statically bounds the size of the dataflow graph a reactive program creates, while still permitting use of higher-order functions and higher-type streams such as streams of streams. We achieve this with a novel linear type theory that both controls allocation and ensures that all recursive definitions are well-founded. We also give a denotational semantics for our language by combining recent work on metric spaces for the interpretation of higher-order causal functions with length-space models of space-bounded computation. The resulting category is doubly closed and hence forms a model of the logic of bunched implications.
Detection of C-reactive protein, streptolysin O, and anti-streptolysin O antibodies in immune complexes isolated from the sera of patients with acute rheumatic fever. (1986)

Circulating immune complexes (IC) of 42 patients with acute rheumatic fever from Santiago, Chile, were studied. The complexes were isolated by polyethylene glycol precipitation and were analyzed for antibodies, antigens, and C-reactive protein. We found the complexes to be enriched in antibody to streptolysin O, particularly in the group of patients with elevated levels of IC. IgM was the predominant class of Ig present in the complexes. Western blots from 12 patients to detect antigens in the complexes showed proteins of m.w. 50,000, 60,000, and 69,000, consistent with the polypeptides of streptolysin O. Such antigens were absent in the complexes from patients with post-streptococcal glomerulonephritis and pharyngitis. Eluted antibodies from these protein bands on the nitrocellulose sheets reacted with the streptolysin O in Western blots and neutralized the hemolytic activity of streptolysin O in a microhemolysin assay. In addition, isolated complexes from several sera showed the presence of C-reactive protein bound to complexes. In vitro experiments demonstrated that [125I]C-reactive protein was not precipitated by polyethylene glycol either alone or when added to monomeric IgG, whereas it precipitated significantly when added to aggregated IgG. The detectable C-reactive protein in isolated complexes and sera samples increased after treatment with sodium dodecyl sulfate. These data suggest that circulating immune complexes in acute rheumatic fever contain streptolysin O and its antibody and raise interesting questions regarding the pathogenetic significance of C-reactive protein in the complexes.
A Ring-Focus Antenna with Splash Plate in Ka-Band (2018)

A design for Ka-band satellite communication antenna in earth station is introduced. This kind of antenna uses splash plate feed based on ring-focus antenna. The theory of the new splash plate feed design is presented and the relative equations are derived. The measured antenna parameters, VSWR, and patterns are highly consistent with the simulated results, which demonstrates the success of the design for such kind of antenna that can be effectively used in Ka-band applications.
NMR Assignment of Rhodobacter capsulatus Ferricytochrome c', a 28 kDa Paramagnetic Heme Protein (1995)

The cytochromes c' are paramagnetic heme proteins generally consisting of two identical 14 kDa subunits. The 1H and 15N resonances of the ferricytochrome c' from the purple phototrophic bacterium Rhodobacter capsulatus have been extensively assigned by the TOCSY-HSQC, NOESY-HSQC, HSQC-NOESY-HSQC, and HNHA 3D heteronuclear experiments performed on an 8 mM sample labeled with 15N. In addition, the 13C alpha and 13CO resonances were assigned by the HNCA and multiple-quantum HNCOCA 3D experiments performed on a 0.5 mM sample labeled with 13C and 15N. The assignment of the backbone 13C resonances was used to confirm the 1H and 15N assignments and to better define secondary structure. On the basis of medium-range NOEs, 3JHN alpha coupling constants, and backbone 13C chemical shifts, the secondary structure consists of four helices: helix-1 (3-29), helix-2 (33-49), helix-3 (78-97), and helix-4 (103-117). On the basis of long-range NOE contacts, the Rb. capsulatus ferricytochrome c' is a four-helix bundle protein in which consecutive helices are antiparallel with respect to one another.
Precursor of amyloid protein in Alzheimer disease undergoes fast anterograde axonal transport. (1990)

In the brains of aged humans and cases of Alzheimer disease, deposits of amyloid in senile plaques are located in proximity to nerve processes. The principal component of this extracellular amyloid is beta/A4, a peptide derived from a larger amyloid precursor protein (APP), which is actively expressed in brain and systemic organs. Mechanisms that result in the proteolysis of APP to form beta/A4, previously termed beta-amyloid protein, and the subsequent deposition of the peptide in brain are unknown. If beta/A4 in senile plaques is derived from neuronally synthesized APP and deposited at locations remote from sites of synthesis, then APP must be transported from neuronal cell bodies to distal nerve processes in proximity to deposits of amyloid. In this study, using several immunodetection methods, we demonstrate that APP is transported axonally in neurons of the rat peripheral nervous system. Moreover, our investigations show that APP is transported by means of the fast anterograde component. These findings are consistent with the hypothesis of a neuronal origin of beta/A4, in which amyloid is deposited in the brain parenchyma of aged individuals and cases of Alzheimer disease. In this setting, we suggest that APP is synthesized in neurons and delivered to dystrophic nerve endings, where subsequent alterations of local processing of APP result in deposits of brain amyloid.
Equiprobable Subcarrier Activation Method for OFDM With Index Modulation (2016)

Orthogonal frequency division multiplexing with index modulation (OFDM-IM) conveys additional information bits via the indices of active subcarriers. Consequently, the determination of the active subcarriers according to the incoming bits arises as a challenging problem. The existing solution resorts to the lexicographic ordering, which leads to a low implementation complexity but an unequal subcarrier activation probability. This letter proposes a distinct solution that allows the equiprobable activation of all OFDM-IM subcarriers with comparable implementation complexity. The signal-to-noise ratio gain achieved by the proposed solution is also analyzed. Computer simulations reveal the advantages of the proposed solution.
Method of measuring the dispersion in multimode optical fibers (1980)

A description is given of an apparatus and method for measuring the transmission characteristics of glass optical fibers, enabling the broadening of 10−9–10−10 sec pulses to be determined with an accuracy of 5×10−12sec. The results are given of measurements of the pulse shape at the output of a multimode fiber having an M-shaped refractive index profile under different conditions of matching the fiber to the source and radiation detector. It is shown that three mode groups propagate in the investigated fiber. This leads to a splitting of a single input radiation pulse into three output pulses, separated in time, when all the modes are excited at the fiber input and the length of the fiber is less than its mode-excitation length.