gebhart/patrepeval huggingface dataset:
Quantitation of the binding, uptake, and degradation of fluoresceinylated neoglycoproteins by flow cytometry (1987)

The fluorescence properties of the fluorescein residues bound to a protein are used to analyze by flow cytometry the neoglycoproteins' endocytosis mediated by membrane lectins of Lewis lung carcinoma cells (3LL cells). The quantum yield of fluorescein bound to a protein is dependent on the number of fluorophore molecules bound to a protein molecule and the pH of the environmental medium. The mean fluorescence intensity of a fluorescein molecule bound to a protein decreases when the number of fluorescein residues per protein molecule increases. However, after proteolytic digestion, the mean fluorescence intensity of a fluorescein molecule is constant and equal to that of free fluorescein. The binding of fluorescein-labeled alpha-glucosylated serum albumin to 3LL cells at 4 degrees C can easily be determined by flow cytometry because under these conditions the environmental pH is neutral, and the neoglycoprotein is not degraded. When the cells are incubated at 37 degrees C in the presence of a fluorescein-labeled neoglycoprotein, the fluorescence intensity of a cell is low because of the low pH of endosomes and lysosomes but is increased upon a postincubation at 4 degrees C in the presence of monensin, a proton/sodium ionophore. The extent of the proteolytic digestion of an endocytosed neoglycoprotein can be assessed by comparing, upon a monensin postincubation at 4 degrees C, the high cell-associated fluorescence of cells incubated in the absence of leupeptin (an inhibitor of lysosomal proteases) and the relatively low fluorescence intensity of cells incubated in the presence of leupeptin.
Optically Active 1,1′-Di-<i>tert</i>-butyl-2,2′-diphosphetanyl and Its Application in Rhodium-Catalyzed Asymmetric Hydrogenations (2004)

(1S,1′S,2R,2′R)-1,1′-Di-tert-butyl-2,2′-diphosphetanyl was prepared from tert-butylphosphine via phosphine-boranes as intermediates. The rhodium complex of the ligand was used as a highly efficient catalyst in asymmetric hydrogenations of α-acetyl­aminoacrylates and α-substituted enamides.
Effect of ethyl apovincaminate on cerebral circulation of dogs under normal conditions and in arterial hypoxia. (1976)

Effects of 1, 2 and 4 mg/kg i.v. doses of ethyl apovincaminate (RGH-4405, Cavinton) on cerebral blood flow, determined in the internal carotid and vertebral artery, were studied in anaesthetized dogs under 21, 16 and 11% O2 inhalation. Total cerebral blood flow was increased by 4 mg/kg Cavinton under normal O2 and between 100 to 200 mmHg perfusion pressures, while 2 mg/kg doses were effective under hypoxia. Similar phenomena were observed in the carotid and vertebral flow, separately. Arterial hypoxia seems to potentiate the effect of Cavinton on cerebral blood flow.
Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid (1990)

The polymerase chain reaction was used to detect Treponema pallidum in specimens of cerebrospinal fluid (CSF), as a means of diagnosing syphilis. Segments of the TmpA and 4D genes were amplified to provide an estimated threshold sensitivity of approximately 65 organisms in 0.5 ml. A spectrum of pathogens known to cause meningitis, and several non-pathogenic treponemes were unreactive. Treponema pertenue, and only one of 30 control specimens of CSF were positive. In contrast, 10 of 19 CSFs from patients being evaluated for latent or tertiary syphilis were positive, as were 7 of 28 specimens from HIV-positive patients.
Survey on Current State-of-the-Art in Needle Insertion Robots: Open Challenges for Application in Real Surgery (2013)

Minimally invasive percutaneous treatment robots have become a popular area in medical robotics. Minimally invasive treatments are an important part of modern surgery; however percutaneous treatments are a difficult procedure for surgeons. They must carry out a procedure that has limited visibility, tool maneuverability and where the target and tissue surrounding it move because of the tool. Robot technology can overcome those limitations and increase the success of minimally invasive percutaneous treatment. In this paper we will present a review of the current state-of-the-art in robotic insertion needle for minimally invasive treatments, focusing on the limitations and challenges still open for their use in clinical application.
Secure Physical Enclosures from Covers with Tamper-Resistance (2018)

Ensuring physical security of multiple-chip embedded systems on a PCB is challenging, since the attacker can control the device in a hostile environment. To detect physical intruders as part of a layered approach to security, it is common to create a physical security boundary that is difficult to penetrate or remove, e.g., enclosures created from tamper-respondent envelopes or covers. Their physical integrity is usually checked by active sensing, i.e., a battery-backed circuit continuously monitors the enclosure. However, adoption is often hampered by the disadvantages of a battery and due to specialized equipment which is required to create the enclosure. In contrast, we present a batteryless tamper-resistant cover made from standard flexPCB technology, i.e., a commercially widespread, scalable, and proven technology. The cover comprises a fine mesh of electrodes and an evaluation unit underneath the cover checks their integrity by detecting short and open circuits. Additionally, it measures the capacitances between the electrodes of the mesh. Once its preliminary integrity is confirmed, a cryptographic key is derived from the capacitive measurements representing a PUF, to decrypt and authenticate sensitive data of the enclosed system. We demonstrate the feasibility of our concept, provide details on the layout, electrical properties of the cover, and explain the underlying security architecture. Practical results including statistics over a set of 115 flexPCB covers, physical attacks, and environmental testing support our design rationale. Hence, our work opens up a new direction of counteracting physical tampering without the need of batteries, while aiming at a physical security level comparable to FIPS 140-2 level 3.
Hypoglycemia-Associated Autonomic Failure in Advanced Type 2 Diabetes (2002)

We tested the hypotheses that the glucagon response to hypoglycemia is reduced in patients who are approaching the insulin-deficient end of the spectrum of type 2 diabetes and that recent antecedent hypoglycemia shifts the glycemic thresholds for autonomic (including adrenomedullary epinephrine) and symptomatic responses to hypoglycemia to lower plasma glucose concentrations in type 2 diabetes. Hyperinsulinemic stepped hypoglycemic clamps (85, 75, 65, 55, and 45 mg/dl steps) were performed on two consecutive days, with an additional 2 h of hypoglycemia (50 mg/dl) in the afternoon of the first day, in 13 patients with type 2 diabetes---7 treated with oral hypoglycemic agents (OHA R(X); mean [+/- SD] HbA(1c) 8.6 +/- 1.1%) and 6 requiring therapy with insulin for an average of 5 years and with reduced C-peptide levels (insulin R(X), HbA(1c) 7.5 +/- 0.7%)---and 15 nondiabetic control subjects. The glucagon response to hypoglycemia was virtually absent (P = 0.0252) in the insulin-deficient type 2 diabetic patients (insulin R(X) mean [+/- SE] final values of 52 plus minus 16 vs. 93 plus minus 15 pg/ml in control subjects and 98 +/- 16 pg/ml in type 2 diabetic patients, OHA R(X) on day 1). Glucagon (P = 0.0015), epinephrine (P = 0.0002), and norepinephrine (P = 0.0138) responses and neurogenic (P = 0.0149) and neuroglycopenic (P = 0.0015) symptom responses to hypoglycemia were reduced on day 2 after hypoglycemia on day 1 in type 2 diabetic patients; these responses were not eliminated, but their glycemic thresholds were shifted to lower plasma glucose concentrations. In addition, the glycemic thresholds for these responses were at higher-than-normal plasma glucose concentrations (P = 0.0082, 0.0028, 0.0023, and 0.0182, respectively) at baseline (day 1) in OHA R(X) type 2 diabetic patients, with relatively poorly controlled diabetes. Because the glucagon response to falling plasma glucose levels is virtually absent and the glycemic thresholds for autonomic and symptomatic responses to hypoglycemia are shifted to lower glucose concentrations by recent antecedent hypoglycemia, patients with advanced type 2 diabetes, like those with type 1 diabetes, are at risk for hypoglycemia-associated autonomic failure and the resultant vicious cycle of recurrent iatrogenic hypoglycemia.
Further complexities of the Rh antigen D disclosed by testing category D<sup>II</sup> cells with monoclonal anti‐D (1993)

The reaction pattern of monoclonal anti-D with category DII cells differed from those of other category D cells. DII cells express epD1, epD2, epD3, epD5, epD6/7 and epD8 but lack epD4 and a new epitope epD9. The new epitope, epD9, is proposed to explain the failure of some monoclonal anti-D (previously considered to be anti-epD3) to react with DII cells.
A Multi-Channel Stopped-Flow Reactor for Measuring Ozone Decay Rate: Instrument Development and Application (2007)

Abstract The ability to accurately estimate ozone decay rate through empirical measurement is often fundamental to efficient reactor design and operation in ozone disinfection processes. Variations of ozone decay rates in natural waters and difficulty to estimate them through mechanistic approach highlight the need for reliable methodologies to measure these parameters. In this study, a novel, multi-channel stopped-flow reactor that allows for automatic, continuous analysis of ozone decay kinetics under controlled conditions was developed and verified. Data presented includes instrument design and operation parameter optimization, precision and accuracy verification, and applicability demonstrated with representative synthetic waters. Keywords: OzoneOzone DecayMulti-Channel Stopped-Flow ReactorMeasurement
Sugar composition and FT‐IR analysis of exopolysaccharides produced by microbial isolates from paper mill slime deposits (2005)

Thirty exopolysaccharides (EPS) produced by bacteria isolated from biofilms or slimelayers from different paper and board mills in Finland, France and Spain were subjected to size exclusion chromatography and sugar compositional analysis. High performance size exclusion chromatography (HPSEC) analysis revealed that some samples were composed of several molecular weight populations. These samples were fractionated by size exclusion chromatography and pooled accordingly. Principal components analysis (PCA) of the sugar compositions of the different pools indicated the presence of glucans and mannans caused by insufficient removal of the carbon or nitrogen source (yeast extract) from the bacteria growth medium leading to an overestimation of the glucose and mannose level in the sample, respectively. From the point of view of slime problems the EPS populations are the most important for multivariate analysis. Four groups of EPSs have been recognized by PCA analysis: a group of EPSs produced by Enterobacter and related genera similar to the regularly reported colanic acid; a group of Methylobacterium EPSs having high galactose and pyruvate levels and two groups that showed less dense clusters produced by Bacillus and related genera, showing high mannose and/or glucose levels and Klebsiella EPSs that showed galactose with rhamnose as major characteristic sugar moieties. Fourier transform infrared spectroscopy (FT-IR) of the same samples followed by discriminant partial least squares regression (DPLS) and linear discriminant analysis (LDA) showed that, when used with a well-defined training set, FT-IR could be used clustering instead of time-consuming sugar composition analysis. The Enterobacter and Methylobacetrium EPS groups could be recognized clearly. However the fact that this could hardly be done for the other two groups in the dataset indicates the importance of a larger and well-defined training or calibration set. The potential to use FT-IR, as a tool for pattern recognition and clustering with respect to EPS structures produced by micro organisms isolated from a paper mill environment is discussed.
A 2.4-GHz CMOS Power Amplifier With an Integrated Antenna Impedance Mismatch Correction System (2014)

To prevent the performance degradation of a power amplifier (PA) from an antenna impedance mismatch, we propose a fully integrated PA with an automatic antenna-mismatch correction system. Using only voltage amplitude information, this method reduces the complexity of the system while compensating impedance mismatch at all mismatched impedance angles. The proposed PA is implemented in 0.18- μm CMOS technology and the measurement results show it maintains its 1-dB gain variation point as well as power-added efficiency under the mismatched condition close to that of a well-matched condition. To the best of our knowledge, this is the first fully integrated CMOS PA that is capable of automatically recovering antenna-mismatch conditions without any help from the off-chip components.
Simultaneous detection of 19 <scp>K</scp><i>‐ras</i> mutations by free‐solution conjugate electrophoresis of ligase detection reaction products on glass microchips (2012)

We demonstrate here the power and flexibility of free-solution conjugate electrophoresis (FSCE) as a method of separating DNA fragments by electrophoresis with no sieving polymer network. Previous work introduced the coupling of FSCE with ligase detection reaction (LDR) to detect point mutations, even at low abundance compared to the wild-type DNA. Here, four large drag-tags are used to achieve free-solution electrophoretic separation of 19 LDR products ranging in size from 42 to 66 nt that correspond to mutations in the K-ras oncogene. LDR-FSCE enabled electrophoretic resolution of these 19 LDR-FSCE products by CE in 13.5 min (E = 310 V/cm) and by microchip electrophoresis in 140 s (E = 350 V/cm). The power of FSCE is demonstrated in the unique characteristic of free-solution separations where the separation resolution is constant no matter the electric field strength. By microchip electrophoresis, the electric field was increased to the maximum of the power supply (E = 700 V/cm), and the 19 LDR-FSCE products were separated in less than 70 s with almost identical resolution to the separation at E = 350 V/cm. These results will aid the goal of screening K-ras mutations on integrated "sample-in/answer-out" devices with amplification, LDR, and detection all on one platform.
Identification of genes involved in utilization of acetate and acetoin in <i>Bacillus subtilis</i> (1993)

The Bacillus subtilis ccpA gene has previously been shown to be involved in repression of amyE expression when cells are grown in excess glucose. The region of the B. subtilis chromosome downstream from ccpA was characterized to determine if additional genes involved in carbohydrate metabolism were present. Two open reading frames that exhibited sequence similarity to the Escherichia coli and B. subtilis motA and motB motility genes were found immediately downstream from ccpA; disruption of this region had no effect on growth, sporulation or motility. Two divergent transcriptional units containing the acsA and acuABC genes were also found in this region. The acsA gene encodes acetyl-CoA synthetase, and inactivation of this gene resulted in loss of the ability to utilize acetate as a carbon source for growth or sporulation. Disruption of the acuABC genes resulted in poor growth or sporulation on acetoin or butanediol. The acsA and acuABC promoter sequences were identified by primer extension, and are in close proximity. Two sequences resembling the amyO regulatory target site necessary for glucose repression of amyE were identified in the acsA-acuABC promoter regions.
<i>In vitro</i> release kinetics of proteins from bioactive foams (2003)

This study describes an approach to obtaining 3-D scaffolds for tissue engineering that allows the incorporation and release of biologically active proteins to stimulate cell function. Laminin was adsorbed on the textured surfaces of binary 70S30C (70 mol % SiO(2), 30 mol % CaO) and ternary 58S (60 mol % SiO(2), 36 mol % CaO, 4 mol % P(2)O(5)) foams. The covalent bonds between the binding sites of the proteins and the ligands on the scaffolds' surfaces did not denaturate the proteins. In vitro studies show that the foams modified with chemical groups and coated with laminin were bioactive, as demonstrated by the formation of a crystalline hydroxy carbonate apatite (HCA) layer formed on the surfaces of the foams upon exposure to simulated body fluid (SBF). The release of proteins from the foams also was investigated. Sustained and controlled release from the scaffolds over a 30-day period was achieved. Laminin release from the bioactive foams followed the dissolution rate of the material network. These results suggest that bioactive foams have the potential to act as scaffolds for soft-tissue engineering with a controlled release of proteins that can induce tissue formation or regeneration.
Hg(OAc)<sub>2</sub>·0.1Sc(OTf)<sub>3</sub>-Catalyzed Cycloisomerization of 2-(4-Pentynyl)furan (2007)

[structure: see text]. Although the Hg(OTf)2.3TMU-catalyzed Friedel-Crafts-type reaction of 3-(4-pentynyl)furan afforded the exo cyclization product, the reaction of 2-(4-pentynyl)furan furnished a very low yield. We found a 10:1 mixed reagent of Hg(OAc)2 and Sc(OTf)3 showed remarkable catalytic activity for the latter transformation. The actual reacting species is presumed to be Hg(OAc)(OTf), which is efficiently generated in situ by mixing the two reagents.
Synthesis of surface-functionalized, probe-containing, polymerized vesicles derived from ammonium bromide surfactants (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of surface-functionalized, probe-containing, polymerized vesicles derived from ammonium bromide surfactantsC. Y. Guo, R. R. Shankar, S. H. Cai, J. Q. Wu, S. Abe, Rhys N. Thomas, J. E. Kuo, T. Tarnowski, and H. KiangCite this: Langmuir 1992, 8, 3, 815–823Publication Date (Print):March 1, 1992Publication History Published online1 May 2002Published inissue 1 March 1992https://pubs.acs.org/doi/10.1021/la00039a015https://doi.org/10.1021/la00039a015research-articleACS PublicationsRequest reuse permissionsArticle Views79Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Battery Modeling: A Versatile Tool to Design Advanced Battery Management Systems (2014)

Fundamental physical and (electro) chemical principles of rechargeable battery operation form the basis of the electronic network models developed for Nickel-based aqueous battery systems, including Nickel Metal Hydride (NiMH), and non-aqueous battery systems, such as the well-known Li-ion. Refined equivalent network circuits for both systems represent the main contribution of this paper. These electronic network models describe the behavior of batteries during normal operation and during over (dis) charging in the case of the aqueous battery systems. This makes it possible to visualize the various reaction pathways, including convention and pulse (dis) charge behavior and for example, the self-discharge performance.
Circulating <scp>PD</scp>‐<scp>L</scp>1 in <scp>NSCLC</scp> patients and the correlation between the level of <scp>PD</scp>‐<scp>L</scp>1 expression and the clinical characteristics (2015)

PD-L1 was elevated in advanced NSCLC patients and may play an important role in tumor immune evasion and patient prognosis.
Characteristics of low wattage microwave induced argon plasmas in metals excitation (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCharacteristics of low wattage microwave induced argon plasmas in metals excitationJohn H. Runnels and James H. GibsonCite this: Anal. Chem. 1967, 39, 12, 1398–1405Publication Date (Print):October 1, 1967Publication History Published online1 May 2002Published inissue 1 October 1967https://pubs.acs.org/doi/10.1021/ac60256a014https://doi.org/10.1021/ac60256a014research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Servo detection and control considering servo pattern defects (2012)

Hard disk drive servo system needs to improve its error tolerance at a decreasing signal to noise ratio (SNR), including at elevated fly heights with lower SNR for servo seek operations than during read and write to support the areal density growth. This paper discusses a servo burst error reduction scheme which can effectively reduce burst pattern defect induced servo demodulation errors and enhance the servo burst SNR. We also discuss the significant RRO that needs to be reduced in a drive with pre-patterned servo such as discrete track media or bit patterned media.
Synthesis of macrocyclic analogues of the neuroprotective agent glycyl-l-prolyl-l-glutamic acid (GPE) (2006)

The syntheses of seven macrocyclic analogues of the neuroprotective tripeptide glycyl-L-prolyl-L-glutamic acid (GPE) are described. Macrocycles 6 and 7 mimic the cis conformer of GPE whereas macrocycles 2-5, 8, and 9 mimic the trans conformer of GPE. The macrocyclic peptides of well-defined geometry were prepared via Grubbs ring closing metathesis of an appropriate diene precursor. In turn each of the diene precursors were prepared from the readily available allyl-substituted amino acid building blocks 12, 13, 14, 27, 36 and 51.