gebhart/patrepeval huggingface dataset:
Soluble Protective Antigen from <i>Bordetella Pertussis</i> Prepared with Sodium Desoxycholate (1963)

Summary Treatment of Bordetella pertussis with sodium desoxycholate caused lysis of cells and liberation of the mouse protective antigen in soluble form and with a good yield. Optimum conditions for solubilization of protective antigen were 0.5 to 1% desoxycholate, pH 8.5, 0 to 2°C and a reduction in optical density of 40 to 50%. Approximately 75% of the bacterial dry weight was dissolved under these conditions. However, the soluble fraction was virtually nontoxic in contrast to ultrasonic extracts of the same cells. Millipore filtration was an effective method of sterilizing solutions of protective antigen.
Magnetic-field-dependent photodynamics of single NV defects in diamond: an application to qualitative all-optical magnetic imaging (2012)

Magnetometry and magnetic imaging with nitrogen-vacancy (NV) defects in\ndiamond rely on the optical detection of electron spin resonance (ESR).\nHowever, this technique is inherently limited to magnetic fields that are weak\nenough to avoid electron spin mixing. Here we focus on the high off-axis\nmagnetic field regime for which spin mixing alters the NV defect spin dynamics.\nWe first study in a quantitative manner the dependence of the NV defect optical\nproperties on the magnetic field vector B. Magnetic-field-dependent\ntime-resolved photoluminescence (PL) measurements are compared to a seven-level\nmodel of the NV defect that accounts for field-induced spin mixing. The model\nreproduces the decreases in (i) ESR contrast, (ii) PL intensity and (iii)\nexcited level lifetime with an increasing off-axis magnetic field. We next\ndemonstrate that those effects can be used to perform all-optical magnetic\nimaging in the high off-axis magnetic field regime. Using a scanning NV defect\nmicroscope, we map the stray field of a magnetic hard disk through both PL and\nfluorescence lifetime imaging. This all-optical method for high magnetic field\nimaging at the nanoscale might be of interest in the field of nanomagnetism,\nwhere samples producing fields in excess of several tens of milliteslas are\ntypical.
Role of nitric oxide in the vascular dysfunction of septic shock (1995)

Conference Article| November 01 1995 Role of nitric oxide in the vascular dysfunction of septic shock D. D. Rees D. D. Rees 1Wellcome Foundation Ltd., Langley Court, Beckenham, Kent BR3 3BS, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1995) 23 (4): 1025–1029. https://doi.org/10.1042/bst0231025 Article history Received: July 28 1995 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation D. D. Rees; Role of nitric oxide in the vascular dysfunction of septic shock. Biochem Soc Trans 1 November 1995; 23 (4): 1025–1029. doi: https://doi.org/10.1042/bst0231025 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: IFN-y, interferon-y, IL-1, interleukin-1, IL-6, interleukin-6, l-NMMA, NG-monomethyl-L-arginine, TNF-α, tumour necrosis factor-α This content is only available as a PDF. © 1995 Biochemical Society1995 Article PDF first page preview Close Modal You do not currently have access to this content.
Link-based service customization for NGN (2008)

In this paper we describe a framework to automatically customize an ongoing communication service whenever a terminal discovers a new link (either wireless or wire-line), which can be used for communication as a secondary link to the Internet. This framework makes use of the information that the server can obtain, as well as the ability to communicate using two links simultaneously at the terminal in order to provide more flexibility for the future communication service. This framework is general enough so that it can be applied to either next generation network or 3G mobile networks.
Highly Regioselective Pd-Catalyzed Intermolecular Aminoacetoxylation of Alkenes and Evidence for <i>cis</i>-Aminopalladation and S<sub>N</sub>2 C−O Bond Formation (2006)

Synthetic methods that achieve oxidative 1,2-difunctionalization of alkenes are very powerful in organic chemistry. Here we report the first examples of intermolecular Pd-catalyzed aminoacetoxylation of alkenes with phthalimide as the nitrogen source and PhI(OAc)2 as the stoichiometric oxidant and source of acetate. These reactions are highly regio- and diastereoselective, and mechanistic studies reveal that the reaction proceeds via cis-aminopalladation of the alkene followed by oxidative cleavage of the intermediate Pd-C bond with inversion of stereochemistry.
Generalized predictive control of multivariable linear systems (1987)

In this paper, the generalized predictive control (GPC) algorithm developed by Clarke {2} is extended in order to control multiple-input-multiple-output (MIMO) linear systems. This algorithm is based on the minimization of a multistep criterion involving the future output errors and future control signals. It requires the design of a long range predictor for the controlled process. The theory is developed for a CARIMA model and for the corresponding state space description in innovation form. This last approach is used to analyse the closed-loop system obtained with the GPC.
Ex Vivo Cardiac Allograft Preservation by Continuous Perfusion Techniques (2000)

The current technique of cardiac preservation for clinical transplantation by infusion of cold cardioplegia and immersion of the heart in an isotonic saline bath at 4 degrees C limits safe tissue preservation time to 4 to 6 hours. The myriad of benefits to be gained by extending cardiac preservation time has prompted the search for alternatives to hypothermic immersion of the heart, the most promising of which involves techniques of coronary artery perfusion. Countless studies have shown the benefits of long-term storage of donor hearts by perfusion rather than the immersion technique. Continuous perfusion preservation has three basic advantages over simple immersion. Perfusion preservation with oxygen carrying solutions has the advantage of preventing ischemia, anaerobic metabolism, and reperfusion injury. Second, nutritional supplementation and provision of substrate can be more effectively delivered to myocardial cells. Third, continuous perfusion preservation effects the clearance of metabolic waste products from the coronary circulation. The composition of the ideal perfusion solution and optimal preservation conditions remain incompletely defined.
The Clinical Effectiveness of Pneumococcal Vaccine in the Elderly (1988)

The effectiveness of pneumococcal vaccine in the immunocompetent elderly remains controversial. We report the results of a multicenter, case-control study of 244 controls and 122 patients, aged 55 years and older, hospitalized during a 5-year period with pneumococcal bacteremia, meningitis, or other bacteriologically confirmed pneumococcal infection. Two controls per patient were matched on the basis of admission date, hospital records, and underlying diseases. All subjects were selected without knowledge of immunization status with pneumococcal vaccine, and were excluded if there was evidence for immunosuppression due to disease or iatrogenic causes. The clinical effectiveness of the vaccine was calculated to be 70% (95% confidence intervals [CI], 37% to 86%) in this population, based on a Mantel-Haenszel point estimate of the odds ratio of 0.30 (95% CI, 0.14% to 0.63%; P less than 0.005). The clinical effectiveness of pneumococcal vaccine in preventing pneumococcal infection in the immunocompetent elderly approximates the vaccine's effectiveness in the general immunocompetent population.
Deposition rate in modulated radio-frequency silane plasmas (2000)

Plasma-enhanced chemical-vapor deposition of amorphous silicon by a square-wave amplitude-modulated radio-frequency excitation has been studied by optical emission spectroscopy and plasma modeling. By the modulation, the deposition rate is increased or reduced, depending on the plasma parameters. The increase in the deposition rate in powder-free (α-regime) plasmas is explained by the behavior of the electrons. High-energy electrons cause a large production of radicals at the onset of the plasma, as evidenced by an overshoot in optical emission. This is confirmed by a one-dimensional fluid model. An optimum in the deposition rate at a modulation frequency of about 100 kHz is determined by the decay time of the electron density.
Ni-Composite Microencapsulated Graphite as the Negative Electrode in Lithium-Ion Batteries II: Electrochemical Impedance and Self-Discharge Studies (2000)

Electrochemical impedance and self‐discharge studies were carried out to investigate lithium intercalation into bare and Ni‐coated KS10 graphite. Values of the charge‐transfer resistances, exchange current densities, surface film resistances, and lithium‐ion diffusion coefficients as functions of the state of charge (SOC) all favored the 10 wt % Ni composite KS10 graphite over bare KS10 graphite when these materials were used as the negative electrode in a Li‐ion cell with mixed organic electrolyte. The charge‐transfer resistances were always lower and gave rise to between 26 and 27% larger exchange current densities, which increased from 137 to 614 mA/g as the SOC increased. The surface film resistances for Ni composite KS10 were between 0.02 and 0.05 Ω g, slightly smaller than those of 0.03 to 0.08 Ω g for bare KS10, and both surface film resistances decreased with increasing SOC. The lithium‐ion diffusion coefficients were always slightly larger, ranging between and . Results from the self‐discharge study also favored the 10 wt % Ni composite KS10, which exhibited less capacity loss over a 10 day period compared to bare KS10. © 2000 The Electrochemical Society. All rights reserved.
Experiments in Character-Level Neural Network Models for Punctuation (2017)

We explore character-level neural network models for inferring punctuation from text-only input. Punctuation inference is treated as a sequence tagging problem where the input is a sequence of un-punctuated characters, and the output is a corresponding sequence of punctuation tags. We experiment with six architectures, all of which use a long short-term memory (LSTM) network for sequence modeling. They differ in the way the context and lookahead for a given character is derived: from simple character embedding and delayed output to enable lookahead, to complex convolutional neural networks (CNN) to capture context. We demonstrate that the accuracy of proposed character-level models are competitive with the accuracy of a state-of-the-art word-level Conditional Random Field (CRF) baseline with carefully crafted features.
Ka-band beam steering reflectarray study (2003)

A one-meter diameter, 32 GHz active microstrip reflectarray capable of electronic beam steering has been studied. The 15,500 element array consists of microstrip patch antennas, microstrip phase shifters, and DC bias lines. Specifically, the study focused on the solid-state devices and DC bias layouts for scanning the beam linearly 45/spl deg/ from boresight using 2-bit phase shifters. Three configurations are recommended for the reflectarray with integrated 2-bit phase shifters where the total substrate and cladding mass varies from 8 kg to 14 kg. Additionally, DC power requirements for p-i-n diodes in 1-bit, 2-bit and 3-bit phase shifting circuits are approximately 19 W, 19 W, and 39 W, respectively. The DC power requirement for phase shifters using passive MESFETs is much less than 1 W.
Characteristics of GaAsSb single-quantum-well-lasers emitting near 1.3 μm (2000)

We report data on GaAsSb single-quantum-well lasers grown on GaAs substrates. Room temperature pulsed emission at 1.275 μm in a 1250-μm-long device has been observed. Minimum threshold current densities of 535 A/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> were measured in 2000-μm-long lasers. We also measured internal losses of 2-5 cm/sup -1/, internal quantum efficiencies of 30%-38% and characteristic temperatures T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> of 67/spl deg/C-77/spl deg/C. From these parameters, a gain constant G <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> of 1660 cm/sup -1/ and a transparency current density J/sub tr/ of 134 A/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> were calculated. The results indicate the potential for fabricating 1.3-μm vertical-cavity surface-emitting lasers from these materials.
The role of olfactory ensheating cells in regenerative medicine: review of the literature (2015)

Abstract Olfactory ensheathing cells (OECs) join olfactory axons in their entrance to the central nervous system, representing a unique population of glial cells with functions in olfactory neurogenesis, axonal growth and olfactory bulb formation. Olfactory ensheathing cells have a great potential to induce repair for neural injuries, in central nervous system and peripheral nervous system, existing numerous experimental and clinical studies lately, reporting beneficial effects in anatomical and functional recovery. Studies are also conducted in order to establish possible pro-regenerative effects of the OECs, their potential in tissue repair and ability to modulate the immune system. The aim of this paper was to review the properties of olfactory ensheathing cells and their potential therapeutic role in regenerative medicine.
P-SELECTIN-DEPENDENT ADHESION OF HUMAN CANCER-CELLS - REQUIREMENT FOR COEXPRESSION OF A PSGL-1-LIKE CORE PROTEIN AND THE GLYCOSYLATION PROCESS FOR SIALOSYL-LE(X) OR SIALOSYL-LE(A) (1995)

We studied the cell surface expression of sialosyl-Le(x) (SLe(x)) and sialosyl-Le(a) (SLe(a)) and its correlation with E-selectin- and P-selectin-dependent cell adhesion, employing 12 human cancer cell lines derived from solid tumors and 2 myelogenic leukemic cell lines, HL60 and U937. Among all the cell lines tested, there was a clear correlation between E-selectin-dependent adhesion and degree of SLe(x) and SLe(a) expression. None of the cell lines derived from solid tumors bound significantly to P-selectin, but leukemic cell lines HL60 and U937 bound strongly to P-selectin. The cDNA clone encoding P-selectin glycoprotein ligand-1 (PSGL-1) was transfected into colonic cancer HRT18 and lung cancer PC3 cells, which express SLe(x) and SLe(a) but normally do not bind to P-selectin, although they do bind to E-selectin. The resulting transfectants bound strongly to P-selectin and equally well to E-selectin. A crude mucin fraction extracted from pooled human colonic cancer tissue bound to E-selectin but not to P-selectin. We conclude that tumor cell adhesion to P-selectin is highly dependent on expression of a specific core protein which appropriately assembles a specific carbohydrate to present to P-selectin. In contrast, E-selectin binds promiscuously to various types of SLe(x) and SLe(a) epitopes presented at the cell surface through N-linked, O-linked, or lipid-linked structures.
Development of a computerized database for evaluation of nurse practitioner student clinical experiences in primary health care. Report of three pilot studies. (1999)

A computerized database for the collection of patient encounter information by nurse practitioner students provides insight into the number and diversity of cases seen. Menu-driven data entry and controlled vocabulary in the form of diagnostic clusters provide a mechanism to categorize and analyze the data. Faculty are able to review student clinical experience by quantitative measures such as number of patient encounters, diagnostic cluster of the encounter, and patient demographic data. Qualitative measures such as student's level of responsibility and student prior experience with stated encounter also are included in the database. Data analysis of two interdisciplinary pilots provided comparisons across disciplines of nursing, medicine, and physical therapy. A subsequent nurse practitioner student pilot provided further refinement and a broadened database terminology more inclusive of a nursing perspective. Educational and clinical issues involved in development, maintenance, and future use of the database are discussed.
The Optimum Tapered Transmission Line Matching Section (1956)

The tapered transmission line matching section is analyzed by treating it as a high pass filter. The ideal reflection coefficient characteristic for the taper that gives the smallest pass band tolerance for a given cutoff frequency and vice versa is derived from the expression for the reflection coefficient of the optimum designed n section quarter-wave transformer by taking the limit as n tends to infinity. By neglecting the square of the reflection coefficient in the differential equation for the reflection coefficient on the taper, a synthesis procedure is derived for obtaining an optimum taper. The procedure is similar to that used in designing an optimum line source distribution in antenna theory. Conditions are derived for the maximum allowable pass band reflection and change in impedance level for which the theory remains accurate. For a maimum reflection coefficient of 0.1 in the pass band the theory remains accurate for all frequencies above the cutoff value provided the change in impedance level does not exceed 7.5. This optimum taper is compared with the well-known exponential and Gaussian tapers and is found to be 13.9 per cent and 27 per cent shorter respectively for the same cutoff frequency and pass band tolerance.
Role of beta2-microglobulin in the intracellular transport and surface expression of murine class I histocompatibility molecules (1989)

We have examined the requirement for beta 2-microglobulin (beta 2m) in the intracellular transport of murine class I histocompatibility molecules to the cell surface. R1E cells that are defective in the synthesis of beta 2m were transfected with either the class I H-2Kb or H-2Db genes alone, or together with the beta 2m gene. Kb or Db heavy chains synthesized in the presence of beta 2m were transported rapidly through the cell and expressed efficiently at the cell surface. In the absence of beta 2m, no "free" Kb heavy chains were detectable at the cell surface and their intracellular transport was blocked at an early stage. In contrast, a significant quantity of "free" Db heavy chains could be detected at the cell surface as we have reported previously. However, we have shown here that defects in intracellular transport were apparent in that the majority (approximately 70%) of newly synthesized Db heavy chains accumulated intracellularly and were degraded. Therefore, although Kb and Db heavy chains differ in their abilities to be expressed at the cell surface in the absence of beta 2m they both require association with beta 2m for efficient intracellular transport. In addition, R1E cells transfected with a deletion construct of the Kb gene expressed a truncated molecule lacking the alpha 3 extracellular domain (Kb-3) at the cell surface, but, like free Db, most newly synthesized Kb-3 molecules accumulated intracellularly. The free Kb, Kb-3, and Db heavy chains were not recognized by most mAb specific for Kb and Db, respectively. Therefore, even the transported forms of free Db and Kb-3 were not native in conformation, which is surprising given the current view that correct folding is essential for intracellular transport. Interestingly, the free Db and Kb-3 heavy chains that reached the cell surface differed in their detergent binding properties from those retained within the cell. This suggests that the transported heavy chains may have folded differently thus allowing their export to the cell surface.
Cooperation between the <i>Hic1</i> and <i>Ptch1</i> tumor suppressors in medulloblastoma (2008)

Medulloblastoma is an embryonal tumor thought to arise from the granule cell precursors (GCPs) of the cerebellum. PATCHED (PTCH), an inhibitor of Hedgehog signaling, is the best-characterized tumor suppressor in medulloblastoma. However, <20% of medulloblastomas have mutations in PTCH. In the search for other tumor suppressors, interest has focused on the deletion events at the 17p13.3 locus, the most common genetic defect in medulloblastoma. This chromosomal region contains HYPERMETHYLATED IN CANCER 1 (HIC1), a transcriptional repressor that is a frequent target of epigenetic gene silencing in medulloblastoma. Here we use a mouse model of Ptch1 heterozygosity to reveal a critical tumor suppressor function for Hic1 in medulloblastoma. When compared with Ptch1 heterozygous mutants, compound Ptch1/Hic1 heterozygotes display a fourfold increased incidence of medulloblastoma. We show that Hic1 is a direct transcriptional repressor of Atonal Homolog 1 (Atoh1), a proneural transcription factor essential for cerebellar development, and show that ATOH1 expression is required for human medulloblastoma cell growth in vitro. Given that Atoh1 is also a putative target of Hh signaling, we conclude that the Hic1 and Ptch1 tumor suppressors cooperate to silence Atoh1 expression during a critical phase in GCP differentiation in which malignant transformation may lead to medulloblastoma.
Reliable multi-destination transfer of data in a local area network (2002)

The author discusses some of the performance aspects of a connection oriented protocol such as TCP/IP or a connectionless protocol especially in terms of their ability to distribute identical data sets to two or more devices in a local area network (LAN). In particular, the author describes an adaptable and reliable multicast data transfer protocol. When compared to an efficient TCP/IP data distribution scheme, the multicast data transfer protocol reduces considerably the time needed to distribute the data and also reduces considerably the load on the LAN. Compared with the TCP/IP method the multicast data transfer protocol requires considerably lower CPU resources for the client but more CPU resources for each of the servers.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Multiple Syringe Inoculator for Agar Plates (1977)

The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface. Construction was simple and inexpensive, with minimal maintenance.