gebhart/patrepeval huggingface dataset:
Methanethiolation of the Liberated Cysteine Residues of Human α<sub>2</sub>‐Macroglobulin Treated with Methylamine Generates a Derivative with Similar Functional Characteristics as Native α<sub>2</sub>‐Macroglobulin (1995)

The thiol-modifying reagent methyl methanethiosulfonate reacts with the cysteine residues of thiol esters released upon treatment of human alpha 2-macroglobulin with methylamine. This methanethiolation generates a derivative of alpha 2-macroglobulin, with an 'open trap' and slow mobility in non-denaturing PAGE, similar to native alpha 2-macroglobulin. This similarity is further substantiated by surface hydrophobicity determinations and by the fact that neither the derivative nor native alpha 2-macroglobulin are cleared from the circulation in mice. Cleavages of bait regions in the derivative and native alpha 2-macroglobulin, however, result in electrophoretically fast forms which are cleared from the circulation in mice. In contrast to native alpha 2-macroglobulin, which can bind 2 mol chymotrypsin/mol, alpha 2-macroglobulin treated with methylamine and methylmethanethiosulfonate binds only 0.8 mol chymotrypsin/mol. Protection of trypsin against inhibition by soybean trypsin inhibitor is significantly better when alpha 2-macroglobulin is modified by methylamine and methylmethanethiosulfonate than when it is modified by dinitrophenyl thiocyanate, which cyanylates the exposed thiol group. The methanethiolated derivative is also more stable than the corresponding cyanylated derivative in that it is transformed to an electrophoretically fast form with a half-life of 9 h as compared to a half-life of 7 h for the latter. The transformation to the fast form is not due to instability of the thiol modification.
Large size MOEMS Fabry-Perot interferometer filter for focal plane array hyperspectral imaging (2015)

Focal plane array (FPA) technology is mature and is widely used for imaging applications. However, FPAs have broadband responses which limit their ability to provide high performance in hyperspectral applications such as detection of buried explosives, and identifying the presence of explosive chemicals and their concentrations. EPIR is currently developing Micro-Opto-Electro-Mechanical System (MOEMS) Fabry-Perot interferometer filter (FPF) devices for FPAs. In this paper, we present our approach to MOEMS FPF design and fabrication that will meet the size requirements for large format FPA hyperspectral imaging. We also report the performance of our FPF resonance cavity, capable of up to 3 μm change gap in tens of nanometer increments.
Extended cyclone theory for gas flows with high solids concentrations (1993)

Abstract Large recirculation cyclones with high concentrations of particulate solids have gained considerable importance in the field of circulating fluidized bed technology. To calculate a combustion process, the influence of the cyclone on the circulating quantity of solids and their grain size distribution must be known. Measurements in a 105‐MW‐plant in 1989 showed that the existing cyclone theory was not sufficient for this purpose. In a laboratory cyclone (900 mm in diameter), the separation and classification of solids in a gas flow was investigated. The cyclone was fed from a fluidized bed and operated with cold air and quartz of various grain size distributions. Based on this experimental device and the theory of momentum transfer between solids and gas flow, a new, more accurate method of calculating the separation efficiency was developed.
The Role of Sampling in Strategic Management Research on Performance: A Two-Study Analysis (2002)

Strategic management researchers often note the inability of many phenomena to consistently explain organizational performance. Using a two-study format, we examine the possibility that sampling practices may contribute to this inability. We chronicle sampling practices within 437 studies investigating the determinants of organizational performance published between 1980 and 1999 in the Academy of Management Journal, Administrative Science Quarterly, Journal of Management, Organization Science, and Strategic Management Journal. Our results reveal that fewer than one in five studies rely on a random sample and that researchers offer little a priori acknowledgement of sample limitations. Although the first study suggests that sampling issues have been problematic, it cannot offer direct evidence. Thus, in the second study, we examine the effect of sample selection on empirical results by testing one popular relationship from the strategy literature (chief executive officer duality and performance) using fou...
Inferring Remote Channel State Information: Cramér-Rao Lower Bound and Deep Learning Implementation (2018)

Channel state information (CSI) is of vital importance in wireless communication systems. Existing CSI acquisition methods usually rely on pilot transmissions, and geographically separated base stations (BSs) with non-correlated CSI need to be assigned with orthogonal pilots which occupy excessive system resources. Our previous work adopts a data-driven deep learning based approach which leverages the CSI at a local BS to infer the CSI remotely, however the relevance of CSI between separated BSs is not specified explicitly. In this paper, we exploit a model-based methodology to derive the Cramér-Rao lower bound (CRLB) of remote CSI inference given the local CSI. Although the model is simplified, the derived CRLB explicitly illustrates the relationship between the inference performance and several key system parameters, e.g., terminal distance and antenna array size. In particular, it shows that by leveraging multiple local BSs, the inference error exhibits a larger power-law decay rate (w.r.t. number of antennas), compared with a single local BS; this explains and validates our findings in evaluating the deep-neural-network-based (DNN-based) CSI inference. We further improve on the DNN-based method by employing dropout and deeper networks, and show an inference performance of approximately $90\%$ accuracy in a realistic scenario with CSI generated by a ray-tracing simulator.
The Cardiotoxic Potential of the 5-HT3 Receptor Antagonist Antiemetics: Is There Cause for Concern? (2002)

As the predicted human life span increases, clinicians will be treating a larger, older oncology population. Because two of the most common major comorbidities are cardiovascular related, we need to be acutely aware of the toxic effects of chemotherapy, as well as the possible cardiac interaction of supportive agents, specifically the 5-HT(3) antiemetics. Until more data are made available, the best antiemetic option for patients receiving emetogenic and cardiotoxic chemotherapy may be the agent with the fewest apparent cardiac effects.
Report of the Immunohistochemistry Steering Committee of the Biological Stain Commission. “Proposed Format: Package Insert for Immunohistochemistry Products” (1992)

(1992). Report of the Immunohistochemistry Steering Committee of the Biological Stain Commission. “Proposed Format: Package Insert for Immunohistochemistry Products”. Biotechnic & Histochemistry: Vol. 67, No. 6, pp. 323-338.
<b>An endosperm‐specific DOF protein from barley, highly conserved in wheat, binds to and activates transcription from the prolamin‐box of a native B‐hordein promoter in barley endosperm</b> (1998)

A cDNA encoding a DNA-binding protein of the DOF class of transcription factors was isolated from a barley endosperm library. The deduced amino acid sequence for the corresponding protein is 94% identical through the DOF domain to the prolamin-box (P-box) binding factor PBF from maize. The gene encoding the barley PBF (BPBF) maps to chromosome 7H, and its expression is restricted to the endosperm where it precedes that of the hordein genes. The BPBF expressed in bacteria as a GST-fusion binds a P-box 5'-TGTAAAG-3' containing oligonucleotide derived from the promoter region of an Hor2 gene. Binding was prevented when the P-box motif was mutated to 5'-TGTAgAc-3'. A P-box binding activity, present in barley and wheat endosperm nuclei, interacted similarly to BPBF with this synthetic oligonucleotide, and the binding was abolished by 1,10-phenanthroline. Transient expression experiments in developing barley endosperms demonstrate that BPBF transactivates transcription from the P-box element of a native Hor2 promoter and that direct binding of BPBF to its target site is essential for transactivation since mutations in the DOF DNA-binding domain or in the P-box motif of this promoter abolished both binding and transactivation. Evidence was also obtained for the presence in wheat of a Pbf homologue having similar DNA-binding properties to that of BPBF. These results strongly implicate this endosperm-specific DOF protein from barley as an important activator of hordein gene expression and suggest the evolutionary conservation of the Pbf gene function among small grain cereals.
Synthesis of Glycosyltransferase Inhibitors (2009)

Glycosyltransferases and glycosidases work together to construct the oligosaccharide moieties of biologically active glycoconjugates. Although many excellent glycosidase inhibitors have been developed, some of which are in clinical use, there are relatively few promising candidates of glycosyltransferase inhibitors. In this review, we summarize the current state of the development of glycosyltransferase inhibitors.
The complex extracellular biology of<i>Streptomyces</i> (2009)

Streptomycetes, soil-dwelling mycelial bacteria that form sporulating aerial branches, have an exceptionally large number of predicted secreted proteins, including many exported via the twin-arginine transport system. Their use of noncatalytic substrate-binding proteins and hydrolytic enzymes to obtain soluble nutrients from carbohydrates such as chitin and cellulose enables them to interact with other organisms. Some of their numerous secreted proteases participate in developmentally significant extracellular cascades, regulated by inhibitors, which lead to cannibalization of the substrate mycelium biomass to support aerial growth and sporulation. They excrete many secondary metabolites, including important antibiotics. Some of these play roles in interactions with eukaryotes. Surprisingly, some antibiotic biosynthetic enzymes are extracellular. Antibiotic production is often regulated by extracellular signalling molecules, some of which also control morphological differentiation. Amphipathic proteins, assembled with the help of cellulose-like material, are required for both hyphal attachment to surfaces and aerial reproductive growth. Comparative genomic analysis suggests that the acquisition of genes for extracellular processes has played a huge part in speciation. The rare codon TTA, which is present in the key pleiotropic regulatory gene adpA and many pathway-specific regulatory genes for antibiotic production, has a particular influence on extracellular biology.
Image enhancement in ultramicroscopy by improved laser light sheets (2010)

In the majority of implementations of light sheet microscopy, such as ultramicroscopy, the laser beam illuminating the specimen is truncated by a slit aperture before it is focused to a light sheet by a single cylindrical lens. A light sheet generated in this way can be made very thin near to the focal point, but unfortunately its Rayleigh range is severely limited. This problem can be partially solved by using a smaller slit aperture. However, this also causes a major loss in power, a severe broadening of the beam waist, and thus a significant loss of resolution along the detection axis. We developed improved light-sheet-generation optics, which provide longer Raleigh ranges, whilst retaining beam waists comparable to our standard system with one cylindrical lens. Using the modified system we achieved a marked improvement in the resolution of ultramicroscopy reconstructions of representative biological specimens.
Laminated bus bars for power system interconnects (1998)

This paper demonstrates the effects of a laminated busbar on power electronics circuits, and compares its characteristics to those of other power distribution topologies. Included in the discussion are physical as well as electrical characteristics, along with the manufacturing implications that need to be considered.
Preventing Bone Loss and Weight Gain with Combinations of Vitamin D and Phytochemicals (2011)

Vitamin D and certain natural compounds have been shown to regulate both lipid metabolism and bone formation. Treatments that prevent or reverse age-related increase in bone marrow adiposity could both increase new bone formation and inhibit bone destruction. We tested the hypothesis that dietary supplementation with combinations of vitamin D and phytochemicals inhibits bone loss and decreases adiposity to a greater extent than control or vitamin D-alone diets. Aged ovariectomized female rats (12 months old, n=50, initial body weight=240 g) were given control (AIN-93M diet), vitamin D (2,400 IU/kg), or vitamin D plus resveratrol (16, 80, or 400 mg/kg of diet [low, medium, and high dose, respectively]), quercetin (80, 400, or 2,000 mg/kg of diet), and genistein (64, 256, or 1,040 mg/kg of diet) for 8 weeks. The high-dose treatment (vitamin D+400 mg/kg resveratrol+2,000 mg/kg quercetin+1,040 mg/kg genistein) reduced body weight gain (P<.05) and the fat pad weights (P<.05). This treatment also increased the serum concentration of insulin-like growth factor-1 (P<.05) and the bone mineral content of the femur. Micro-computed tomography and histomorphometric analyses indicated that the high-dose treatment prevented loss of trabecular bone (P<.05) and reduced marrow adipocytes (P<.001) and osteoclasts (P<.05) compared with the control and vitamin D alone (P<.05). We conclude that aged ovariectomized female rats supplemented with vitamin D combined with genistein, quercetin, and resveratrol had improved bone mineral density and reduced body weight gain and a significant decrease in bone marrow adipocytes. The synergistic effects of a combination of phytochemicals with vitamin D may be effective in reducing bone loss and weight gain after menopause.
Bacteriophage adhering to mucus provide a non–host-derived immunity (2013)

Mucosal surfaces are a main entry point for pathogens and the principal sites of defense against infection. Both bacteria and phage are associated with this mucus. Here we show that phage-to-bacteria ratios were increased, relative to the adjacent environment, on all mucosal surfaces sampled, ranging from cnidarians to humans. In vitro studies of tissue culture cells with and without surface mucus demonstrated that this increase in phage abundance is mucus dependent and protects the underlying epithelium from bacterial infection. Enrichment of phage in mucus occurs via binding interactions between mucin glycoproteins and Ig-like protein domains exposed on phage capsids. In particular, phage Ig-like domains bind variable glycan residues that coat the mucin glycoprotein component of mucus. Metagenomic analysis found these Ig-like proteins present in the phages sampled from many environments, particularly from locations adjacent to mucosal surfaces. Based on these observations, we present the bacteriophage adherence to mucus model that provides a ubiquitous, but non-host-derived, immunity applicable to mucosal surfaces. The model suggests that metazoan mucosal surfaces and phage coevolve to maintain phage adherence. This benefits the metazoan host by limiting mucosal bacteria, and benefits the phage through more frequent interactions with bacterial hosts. The relationships shown here suggest a symbiotic relationship between phage and metazoan hosts that provides a previously unrecognized antimicrobial defense that actively protects mucosal surfaces.
Grain size effect on noise properties in CoCrTa thin film media (1995)

Grain growth of Cr and CoCrTa thin film was examined using high resolution transmission electron microscopy (HRTEM) and high resolution scanning microscopy (HRSEM). The effect of crystallography on media noise was studied. The grain size of the CoCrTa thin film increases with thickness and retains a bi-crystal structure over a range of Cr grain size. Media noise at a high recording density seems to increase with the grain size of Cr underlayer surface. This suggests that the micromagnetization process can be influenced by a bi-crystal structure of Co alloy films at their initial layers.
Suppression of Experimental Autoimmune Uveoretinitis by Anti-IL-17 Antibody (2009)

These findings demonstrate that blockade of endogenous IL-17 activity by treatment with anti-IL-17 antibody attenuates EAU in rats. IL-17 rather than IFN-gamma plays a crucial role in the development of EAU, and that antagonism of IL-17 could be useful for the treatment of human intraocular autoimmune diseases.
Consonant identification in noise using Hilbert-transform temporal fine-structure speech and recovered-envelope speech for listeners with normal and impaired hearing (2015)

Consonant-identification ability was examined in normal-hearing (NH) and hearing-impaired (HI) listeners in the presence of steady-state and 10-Hz square-wave interrupted speech-shaped noise. The Hilbert transform was used to process speech stimuli (16 consonants in a-C-a syllables) to present envelope cues, temporal fine-structure (TFS) cues, or envelope cues recovered from TFS speech. The performance of the HI listeners was inferior to that of the NH listeners both in terms of lower levels of performance in the baseline condition and in the need for higher signal-to-noise ratio to yield a given level of performance. For NH listeners, scores were higher in interrupted noise than in steady-state noise for all speech types (indicating substantial masking release). For HI listeners, masking release was typically observed for TFS and recovered-envelope speech but not for unprocessed and envelope speech. For both groups of listeners, TFS and recovered-envelope speech yielded similar levels of performance and consonant confusion patterns. The masking release observed for TFS and recovered-envelope speech may be related to level effects associated with the manner in which the TFS processing interacts with the interrupted noise signal, rather than to the contributions of TFS cues per se.
Enantioselective Synthesis of (<i>R</i>)‐2‐Methylalkanoic Acids: A Convenient Approach to α‐Substituted Chiral Carboxylic Acid Derivatives (2004)

Abstract Racemization‐free deacylation of N‐acylimidazolidine‐2‐ones using lithium hydroperoxide affords the corresponding α‐substituted chiral carboxylic acids in high yield while permitting recovery of the chiral auxiliary.
THE USE OF NON-STEROIDAL ANTI-INFLAMMATORY DRUGS IN PAEDIATRIC RHEUMATIC DISEASES (1993)

The use of NSAIDs for arthritis differs in children from adults in their indications, uses and pharmacokinetics, and fewer are available. Children with arthritis are assessed differently, as they complain less of pain. Salicylates, indomethacin and ibuprofen are used for the fever of systemic JCA. For control of joint symptoms, diclofenac, ibuprofen, tolmetin and naproxen are equal in their efficacy and tolerance:salicylates and indomethacin are no more effective but more toxic. Children tolerate NSAIDs well. Gastrointestinal symptoms appear to be less common than in adults, but the evidence regarding endoscopic changes in conflicting. Renal toxicity is rare. Tolmetin can cause pseudoproteinuria and naproxen pseudoporphyria. The liver in systemic JCA is vulnerable to drug toxicity. A therapeutic trial of an NSAID should continue for 8 weeks. Interactions with methotrexate and carbonic anhydrase inhibitors for glaucoma complicating iridocyclitis may occur.
Type VI collagen. Studies on its localization, structure, and biosynthetic form with monoclonal antibodies. (1984)

Monoclonal antibodies were prepared by immunization with whole tissue and were selected for their reactivity with extracellular matrices in tissue immunofluorescence. Two such antibodies were used to isolate the corresponding antigen from pepsin extracts of human placental tissue by immunochromatography. In each case, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate showed that the isolated material was composed of four polypeptides of Mr between 57,000 and 85,000 that were disulfide-bonded into a high molecular weight aggregate. Amino acid analyses showed that the isolated material was partly collagenous. The material was shown to be antigenically related to previously isolated peptic fragments of type VI collagen and it shared their unique structure as revealed by electron microscopy. Based on these findings, it was concluded that the isolated material was a form of type VI collagen. In immunofluorescence, the monoclonal antibodies localized type VI collagen throughout the connective tissue and in the extracellular matrix of cultured fibroblasts. Polypeptides presumably comprising the intact form of this collagen were isolated from cultures of metabolically radiolabeled fibroblast cell cultures using the two monoclonal antibodies. The isolated material consisted of two polypeptides of Mr 240,000 and 140,000 that were extensively disulfide cross-linked. Four additional monoclonal antibodies bound the same radioactive polypeptides from fibroblast cultures, but only one of them reacted with the fragments isolated from pepsin-digested placenta. Since all six antibodies were originally selected based on tissue immunofluorescence, and therefore react with the tissue form of the protein, the tissue form appears to be more similar to the polypeptides detected in fibroblast cultures than to the pepsin-resistant fragments. Since these monoclonal antibodies apparently recognize different parts of the molecule, they will be useful for further study of the structure and function of the intact form of type VI collagen.
Si-H and N-H vibrational properties in glow-discharge amorphous SiN<i>x</i>:H films (0&lt;<i>x</i>&lt;1.55) (1990)

Amorphous SiNx:H films were prepared by rf glow discharge of SiH4-NH3 mixtures at 300 °C, and the SiH, NH, and SiN vibrational absorptions were investigated as a function of x. The stretching absorption profiles due to SiH and SiN bonds are reproduced by a superposition of two components at around 2000 and 2100 cm−1, and of three components at around 750, 840, and 960 cm−1, respectively. The dependence of these intensities on x was examined by means of a generation probability analysis on the basis of the random bonding model including SiH and NH bonds which play an important role in the film growth mechanism.
Characterization for Multiharmonic Intermodulation Nonlinearity of RF Power Amplifiers Using a Calibrated Nonlinear Vector Network Analyzer (2016)

This paper presents a novel realization of nonlinear vector network analyzer (NVNA) test-bed with a new phase calibration technique, in order to characterize the multiharmonic intermodulation nonlinearity of a radio frequency (RF) power amplifier (PA) under a large-signal modulated input. The NVNA test-bed uses a step recovery diode comb generator as the phase reference, which is driven by a pulsed-RF signal with a high duty cycle of over 99%. Hence all the multiharmonic intermodulation tones of interest can be measured at once. A multisine phase standard provided by a vector signal analyzer is used to calibrate the intermodulation tones at each harmonic on a dense spectral grid. Two harmonic phase standards with different fundamental frequencies are used to synchronize the phase calibration results at different harmonics. The validity of this paper is verified by comparing NVNA measurements with oscilloscope measurements of the same PA under test. This paper can be used to inspect the nonlinearity of RF amplifiers under large-signal modulated excitations in both the frequency and time domains.