gebhart/patrepeval huggingface dataset:
Relationship between structure of the 5' noncoding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system. (1982)

To determine whether the rate of protein synthesis is controlled by the structure of mRNA near its 5' terminus, protein-synthesizing ability, especially in its initial stage, was compared among RNAs of plant viruses. Those viruses used here contain several definite pieces of single-stranded RNA. Each of these RNAs acts as a messenger. Cucumber mosaic virus (CMV) RNA 5 synthesizes a small amount of a protein, Mr 7000, in an in vitro protein-synthesizing system from wheat germ or reticulocyte. Brome mosaic virus (BMV) RNA 4 synthesizes a large amount of a coat protein under the same conditions. Both RNAs have the same 5'-cap structure and a short noncoding region (10 nucleotides in CMV RNA 5 and 9 in BMV RNA 4) between the 5' terminus and the initiation codon AUG. A sequence complementary to the 3' terminal of 18S ribosomal RNA is contained in BMV RNA 4 but is not apparent in CMV RNA 5. Formation of the initiation complex for protein synthesis by the 5'-terminal-labeled mRNA of cytoplasmic polyhedrosis virus was inhibited by the addition of unlabeled BMV RNA 4 whereas it was only slightly inhibited by unlabeled CMV RNA 5. BMV RNA 4, which has a sequence complementary to rRNA, can form the initiation complex more easily than CMV RNA5. It is concluded that an apparent complementary sequence in the 3' terminal of 18S rRNA in the 5' noncoding region of eukaryotic mRNA and the 5'-cap structure enhance the rate of initiation complex formation in protein synthesis.
MWIR InAsSb XB n n detector (bariode) arrays operating at 150K (2011)

The XB<sub>n</sub><i>n</i> high operating temperature (HOT) detector project at SCD is aimed at developing a HOT (~150K) mid-wave infrared (MWIR) detector array, based on InAsSb/AlSbAs barrier detector or "bariode" device elements. The essential principle of the XB<sub>n</sub><i>n</i> bariode architecture is to suppress the Generation-Recombination contribution to the dark current by ensuring that the depletion region of the device is contained inside a large bandgap <i>n</i>-type barrier layer (BL) and excluded from the narrow bandgap <i>n</i>-type active layer (AL). The band profile of the XB<sub>n</sub><i>n</i> device leads to effective blocking of electron transport across the BL while maintaining a free path for the holes, thus assuring a high internal quantum efficiency (QE). Our devices exhibit a very large minority carrier lifetime (~700 ns), leading to a very low dark current of <10<sup>-6</sup> A cm<sup>-2</sup> at 150K, which is essentially diffusion limited. We compare bariode devices with both a <i>p</i>-type GaSb contact layer (CL) and an n-type InAsSb CL (termed C<sub>p</sub>B<sub>n</sub><i>n</i> and <i>n</i>B<sub>n</sub><i>n</i>, respectively). Apart from a ~0.3V shift in the operating bias, the optical and electrical properties of both architectures are virtually identical, demonstrating the generic nature of the XB<sub>n</sub><i>n</i> barrier detector family. We have fabricated FPAs from <i>n</i>B<sub>n</sub><i>n</i> bariode arrays bonded both to a 320×256, 30 μm pitch Read-Out Integrated Circuit (ROIC) and a 640×512, 15 μm pitch ROIC. For lattice matched FPAs the cut-off wavelength at >50% of maximum response is ~ 4.1 μm. We show an image registered at 150K with a 640×512/15 μm Pelican FPA, using f/3.2 optics. The operability at 150K is >99.5% and the measured NETD, limited only by shot and Read-Out noise, is 20 mK for a 22 ms integration time. At this f/number, the detector has a background limited performance (BLIP) up to ~165K.
Rookhope Old Smelt Mill, Rookhope, County Durham: results of the 1995 survey (2001)

Rookhope Old Smelt Mill (Scheduled Ancient Monument No 29010) was abandoned some time after the construction ofRookhope Smelt Mill 1km to the E by the Blackett company between 1730 and 1740. In 1995, the authors carried out a survey of the site and were able to confirm its use for smelting lead ores. There were one and possibly two smelting mills on the site together with evidence of slag crushing and ore dressing operations. There is a system of leats to supply water to the various operations. The site is overlain to the W by the dam of the newer Rookhope Smelt Mill and the shafts and outlet from the Tailrace Level to the E. In August 1999, the Environment Agency commenced work to build a lagoon to intercept silt carried by water overflowing from the nearby Fraser's Grove mine further up the valley. This work has destroyed much of the old smelt mill site.
Iodine-123-beta-CIT and iodine-123-FPCIT SPECT measurement of dopamine transporters in healthy subjects and Parkinson's patients. (1998)

These data suggest that SPECT imaging with [123I]-FPCIT visually demonstrates reductions in striatal uptake similar to [123I]-beta-CIT. iodine-123-FPCIT washed out from striatal tissue 15-20 times faster than [123I]-beta-CIT, and estimates of dopamine transporter loss in Parkinson's disease patients were higher for [123I]-FPCIT than for [123I]-beta-CIT. This was most likely due to the faster rate of striatal washout and establishment of transient equilibrium binding conditions at the dopamine transporter, which the modeling theory suggests produces an overestimation of dopamine transporter density with relatively greater overestimates in healthy control subjects by [123I]-FPCIT.
El mentiroso y el nuevo esquema T (2010)

Desde que Tarski publicó su estudio sobre el concepto de verdad en los años 30, ha sido una práctica ortodoxa el considerar que toda instancia del esquema T es verdadera. Sin embargo, algunas instancias del esquema son falsas. Éstas incluyen las instancias paradójicas ejemplificadas por la oración del mentiroso. Aquí se demuestra que un esquema mejor permite un tratamiento uniforme de la verdad en el que las paradojas semánticas resultan ser simplemente falsas.
A non-insulated resonant boost converter (2010)

In this paper, a resonant boost converter is analyzed and verified through experimentation. Switching losses are reduced since the converter operates at ZCS (Zero Current Switching). The analysis presented here covers its operation with variable switching frequency but only below the resonant frequency. The range of its voltage gain goes from 1 to 2 and the energy conversion efficiency (including filtering at input) is around 97% for the lowest input voltage. Its envisioned that it could be applied as a front-end converter when a backend inverter is needed, for instance with batteries, photovoltaic converters or fuel cells, perform partial output voltage regulation only.
An analysis of the improvements in OSNR from distributed Raman amplifiers using modern transmission fibres (2002)

We present measurements of the Raman efficiency spectra for recent transmission fibres and model the improvement in DWDM system performance using distributed Raman amplification. For 5 spans of 25 dB fibre loss and 1 dB of connector loss, we predict 4.5-6.5 dB improvement in optical signal to noise ratio (OSNR) when using a 500 mW pump, a variation of up to 2 dB depending on fibre type.
Wolbachia infections and the expression of cytoplasmic incompatibility in Drosophila sechellia and D. mauritiana. (1995)

Various stocks of Drosophila mauritiana and D. sechellia were found to be infected with Wolbachia, a Rickettsia-like bacterium that is known to cause cytoplasmic incompatibility and other reproductive abnormalities in arthropods. Testing for the expression of cytoplasmic incompatibility in these two species showed partial incompatibility in D. sechellia but no expression of incompatibility in D. mauritiana. To determine whether absence of cytoplasmic incompatibility in D. mauritiana was due to either the bacterial or host genome, we transferred bacteria from D. mauritiana into an uninfected strain of D. simulans, a host species known to express high levels of incompatibility with endogenous Wolbachia. We also performed the reciprocal transfer of the natural D. simulans Riverside infection into a tetracycline-treated stock of D. mauritiana. In each case, the ability to express incompatibility was unaltered by the different host genetic background. These experiments indicate that in D. simulans and D. mauritiana expression of the cytoplasmic incompatibility phenotype is determined by the bacterial strain and that D. mauritiana harbors a neutral strain of Wolbachia.
Surface Roughness Analysis and Measurement Techniques (2000)

A solid surface, or more exactly a solid-gas or solid-liquid interface, has a complex structure and complex properties depending on the nature of the solids, the method of surface preparation, and the interaction between the surface and the environment. Properties of solid surfaces are crucial to surface interaction because surface properties affect real area of contact, friction, wear, and lubrication. In addition to tribological functions, surface properties are important in other applications, such as optical, electrical and thermal performance, painting, and appearance.
DeepOrganNet: On-the-Fly Reconstruction and Visualization of 3D / 4D Lung Models from Single-View Projections by Deep Deformation Network (2019)

This paper introduces a deep neural network based method, i.e., DeepOrganNet, to generate and visualize fully high-fidelity 3D / 4D organ geometric models from single-view medical images with complicated background in real time. Traditional 3D / 4D medical image reconstruction requires near hundreds of projections, which cost insufferable computational time and deliver undesirable high imaging / radiation dose to human subjects. Moreover, it always needs further notorious processes to segment or extract the accurate 3D organ models subsequently. The computational time and imaging dose can be reduced by decreasing the number of projections, but the reconstructed image quality is degraded accordingly. To our knowledge, there is no method directly and explicitly reconstructing multiple 3D organ meshes from a single 2D medical grayscale image on the fly. Given single-view 2D medical images, e.g., 3D / 4D-CT projections or X-ray images, our end-to-end DeepOrganNet framework can efficiently and effectively reconstruct 3D / 4D lung models with a variety of geometric shapes by learning the smooth deformation fields from multiple templates based on a trivariate tensor-product deformation technique, leveraging an informative latent descriptor extracted from input 2D images. The proposed method can guarantee to generate high-quality and high-fidelity manifold meshes for 3D / 4D lung models; while, all current deep learning based approaches on the shape reconstruction from a single image cannot. The major contributions of this work are to accurately reconstruct the 3D organ shapes from 2D single-view projection, significantly improve the procedure time to allow on-the-fly visualization, and dramatically reduce the imaging dose for human subjects. Experimental results are evaluated and compared with the traditional reconstruction method and the state-of-the-art in deep learning, by using extensive 3D and 4D examples, including both synthetic phantom and real patient datasets. The efficiency of the proposed method shows that it only needs several milliseconds to generate organ meshes with 10K vertices, which has great potential to be used in real-time image guided radiation therapy (IGRT).
Studies on extrarenal erythropoietin. (1969)

Abstract The plasma of nephrectomized rats exposed to intense hypoxia contained detectable amounts of an erythropoietic stimulating factor. The titer of this factor increased with further increase in the intensity of hypoxia. Extrarenal erythropoietin is capable of stimulating heme synthesis by bone marrow cells in vitro and is neutralized by antiserum to erythropoietin. These data indicate that extrarenal erythropoietin is probably identical to that of renal origin, and its production is regulated by the intensity of hypoxia.
Defined Conjugation of Glycans to the Lysines of CRM<sub>197</sub> Guided by their Reactivity Mapping (2014)

Systematic characterisation of the reactivity of the lysine moieties in CRM197 towards N-hydroxysuccinimide linkers bearing alkynes or azides is described. This involves two-step conjugation of various glycans to CRM197 by click chemistry in a well-defined manner. By semiquantitative LC-MS/MS analysis of proteolytic digests of the conjugates formed, the reactivity of lysine residues in the protein was mapped and ranked. Computational analysis of the solvent accessibility of each lysine residue (based on the CRM197 crystal structure) established a correlation between reactivity and surface exposure. By this approach, conjugation involving lysine residues (normally a random process) can be controlled. It enables the preparation of lysine-mediated glycoconjugates with improved batch-to-batch reproducibility, thereby producing neo-glycoconjugates with more-consistent biological activity.
Retinoic Acid Receptor β Stimulates Hepatic Induction of Fibroblast Growth Factor 21 to Promote Fatty Acid Oxidation and Control Whole-body Energy Homeostasis in Mice (2013)

Activation of retinoic acid receptor (RAR) with all-trans-retinoic acid (RA) ameliorates glucose intolerance and insulin resistance in obese mice. The recently discovered fibroblast growth factor 21 (FGF21) is a hepatocyte-derived hormone that restores glucose and lipid homeostasis in obesity-induced diabetes. However, whether hepatic RAR is linked to FGF21 in the control of lipid metabolism and energy homeostasis remains elusive. Here we identify FGF21 as a direct target gene of RARβ. The gene transcription of Fgf21 is increased by the RAR agonist RA and by overexpression of RARα and RARβ, but it is unaffected by RARγ in HepG2 cells. Promoter deletion analysis characterizes a putative RA-responsive element (RARE) primarily located in the 5'-flanking region of the Fgf21 gene. Disruption of the RARE sequence abolishes RA responsiveness. In vivo adenoviral overexpression of RARβ in the liver enhances production and secretion of FGF21, which in turn promotes hepatic fatty acid oxidation and ketogenesis and ultimately leads to increased energy expenditure in mice. The metabolic effects of RA or RARβ are mimicked by FGF21 overexpression and largely abolished by FGF21 knockdown. Moreover, hepatic RARβ is bound to the putative RAREs of the Fgf21 promoter in a fasting-inducible manner in vivo, which contributes to FGF21 induction and the metabolic adaptation to prolonged fasting. In addition to other nuclear receptors, such as peroxisome proliferator-activated receptor α and retinoic acid receptor-related receptor α, RAR may act as a novel component to induce hepatic FGF21 in the regulation of lipid metabolism. The hepatic RAR-FGF21 pathway may represent a potential drug target for treating metabolic disorders.
Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits (2019)

Macroautophagy/autophagy is generally regarded as a cytoprotective mechanism, and it remains a matter of controversy whether autophagy can cause cell death in mammals. Here, we show that chronic restraint stress suppresses adult hippocampal neurogenesis in mice by inducing autophagic cell death (ACD) of hippocampal neural stem cells (NSCs). We generated NSC-specific, inducible <i>Atg7</i> conditional knockout mice and found that they had an intact number of NSCs and neurogenesis level under chronic restraint stress and were resilient to stress- or corticosterone-induced cognitive and mood deficits. Corticosterone treatment of adult hippocampal NSC cultures induced ACD via SGK3 (serum/glucocorticoid regulated kinase 3) without signs of apoptosis. Our results demonstrate that ACD is biologically important in a mammalian system <i>in vivo</i> and would be an attractive target for therapeutic intervention for psychological stress-induced disorders.<b>Abbreviations</b>: AAV: adeno-associated virus; ACD: autophagic cell death; ACTB: actin, beta; Atg: autophagy-related; ASCL1/MASH1: achaete-scute family bHLH transcription factor 1; BafA<sub>1</sub>: bafilomycin A<sub>1</sub>; BrdU: Bromodeoxyuridine/5-bromo-2'-deoxyuridine; CASP3: caspase 3; cKO: conditional knockout; CLEM: correlative light and electron microscopy; CORT: corticosterone; CRS: chronic restraint stress; DAB: 3,3'-diaminobenzidine; DCX: doublecortin; DG: dentate gyrus; GC: glucocorticoid; GFAP: glial fibrillary acidic protein; HCN: hippocampal neural stem; i.p.: intraperitoneal; MAP1LC3B: microtubule-associated protein 1 light chain 3 beta; MKI67/Ki67: antigen identified by monoclonal antibody Ki 67; MWM: Morris water maze; Nec-1: necrostatin-1; NES: nestin; NR3C1/GR: nuclear receptor subfamily 3, group C, member 1; NSC: neural stem cell; PCD: programmed cell death; PFA: paraformaldehyde; PX: Phox homology; PtdIns3P: phosphatidylinositol-3-phosphate; RBFOX3/NeuN: RNA binding protein, fox-1 homolog (C. elegans) 3; SGK: serum/glucocorticoid-regulated kinases; SGZ: subgranular zone; SOX2: SRY (sex determining region Y)-box 2; SQSTM1: sequestosome 1; STS: staurosporine; TAM: tamoxifen; Ulk1: unc-51 like kinase 1; TUNEL: terminal deoxynucleotidyl transferase dUTP nick end labeling; VIM: vimentin; WT: wild type; ZFYVE1: zinc finger, FYVE domain containing 1; Z-VAD/Z-VAD-FMK: pan-caspase inhibitor.
INHIBITION OF (Na,K)ATPase IN THYMOCYTES REDUCES APOPTOSIS INDUCED BY MICROTUBULE-DISRUPTING AGENTS (1996)

Conference Abstract| November 01 1996 INHIBITION OF (Na,K)ATPase IN THYMOCYTES REDUCES APOPTOSIS INDUCED BY MICROTUBULE-DISRUPTING AGENTS Bogdan Djuričić; Bogdan Djuričić 1Institutes of Biochemistry and Histology, Faculty of Medicine, Belgrade, Yugoslavia Search for other works by this author on: This Site PubMed Google Scholar Andjelija Škaro-Milić; Andjelija Škaro-Milić 1Institutes of Biochemistry and Histology, Faculty of Medicine, Belgrade, Yugoslavia Search for other works by this author on: This Site PubMed Google Scholar Dejan Arsić; Dejan Arsić 1Institutes of Biochemistry and Histology, Faculty of Medicine, Belgrade, Yugoslavia Search for other works by this author on: This Site PubMed Google Scholar Vladimir Bumbaširević Vladimir Bumbaširević 1Institutes of Biochemistry and Histology, Faculty of Medicine, Belgrade, Yugoslavia Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1996 Biochemical Society1996 Biochem Soc Trans (1996) 24 (4): 612S. https://doi.org/10.1042/bst024612sa Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Bogdan Djuričić, Andjelija Škaro-Milić, Dejan Arsić, Vladimir Bumbaširević; INHIBITION OF (Na,K)ATPase IN THYMOCYTES REDUCES APOPTOSIS INDUCED BY MICROTUBULE-DISRUPTING AGENTS. Biochem Soc Trans 1 November 1996; 24 (4): 612S. doi: https://doi.org/10.1042/bst024612sa Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1996 Biochemical Society1996 Article PDF first page preview Close Modal You do not currently have access to this content.
Relaxin regulation of endometrial structure and function in the rhesus monkey (2004)

Despite the documented importance of the protein hormone relaxin in reproduction in various mammalian species, the role of relaxin in human reproduction is poorly understood, largely because of the lack of studies in women or in suitable non-human primate models. Here we describe the establishment of a non-human primate model of early human pregnancy and its use in defining the actions of relaxin. Results demonstrate that relaxin exerts dramatic uterine effects including pronounced increase in uterine weight and stimulation of endometrial angiogenesis and resident endometrial lymphocyte number. In addition, relaxin decreases endometrial levels of matrix metalloproteinases 1 and 3 and increases levels of their endogenous inhibitor, tissue inhibitor of metalloproteinase 1, resulting in maintenance of endometrial collagen content. Relaxin significantly inhibits endometrial levels of estrogen receptor alpha, but not beta, and of progesterone receptor isoforms A and B. The findings that relaxin stimulates new blood vessel formation and increases cytokine-containing lymphocyte number while maintaining endometrial connective tissue integrity are consistent with a significant role of relaxin in the establishment and/or maintenance of early pregnancy.
Heterogeneously Integrated III-V/Si Distributed Bragg Reflector Laser with Adiabatic Coupling (2013)

We report on a III-V on Silicon distributed Bragg reflector laser with adiabatic coupling operating continuous wave at 1547 nm. The lasing threshold at 20 °C and the maximum output power are 17 mA and 15 mW, respectively. The fiber-coupled power is higher than 4 mW. The device is directly modulated and generates open eye-diagram up to 12.5 Gb/s.
PURIFICATION AND CHARACTERIZATION OF ANTIOXIDANT PEPTIDES FROM SOY PROTEIN HYDROLYSATE (2010)

ABSTRACT Soy protein hydrolysates (SPH) were obtained by Alcalase hydrolysis of soy protein. Antioxidant activity of SPH was measured with a lipid peroxidation inhibitory activity and lipid oxidation was evaluated by measuring the thiobarbituric acid (TBA) and peroxide value. The potent antioxidant peptide was purified using ultrafiltration and consecutive chromatographic methods including FPLC and reverse-phase high-performance liquid chromatography. The antioxidant activity having a specific activity of 108.13%/µg for TBA method was enhanced 67.6-fold compared with SPH. In the amino acid composition of the final potent antioxidant peptide, hydrophobic amino acids were the most abundant amino acids and among them phenylalanine was especially abundant. PRACTICAL APPLICATIONS Soybean is important because of not only its multiple health-promoting properties but also its role as a useful source for producing biological active peptides. Enzymatic hydrolysis has been employed in order to produce the active peptide from various protein sources because of its naturalness and safety. In this paper, the potent antioxidant peptide was purified by an ultrafiltration and a series of chromatography from soy protein hydrolysates (SPH) obtained by Alcalase treatment. It is expected that the SPH with enhanced antioxidant activity could be used in a wide range of foods as a useful functional food ingredient.
Effect of MnO2 on Properties of NiFe2O4 Spinel Based Inert Anode (2005)

Abstract: In order to improve the properties of NiFe_2O_4 spinel based inert anode, some additive MnO_2 were added to raw materials. NiFe_2O_4 spinel with MnO_2 was made by solid-phase reaction at 1200℃ for 6 h. XRD were carried out and the effects of MnO_2 on density, conductivity and corrosion resistance were measured. XRD shows when MnO_2 was added no new phases exist and MnO_2 and NiFe_2O_4 formed solid solution; Mn~ 4+ replaced parts of Fe~ 3+ and the sample still had the structure of NiFe_2O_4 spinel. The crystal lattice of NiFe_2O_4 spinel became aberrated when MnO_2 was added, which can promote sintering, and improve density. Because Mn~ 4+ replaces parts of Fe~ 3+ and produces conduction electron, which can improve conductivity. The corrosion resistance of the samples was enhanced. When MnO_2 is 1.0%, the sample's corrosion rate is 1/5 of that of the sample without MnO_2. The reason is that Al_2O_3 in the melt reacts with Mn~ 4+ in the sample to produce MnAl_2O_4. MnAl_2O_4 forms a dense protecting coat, which can prevent melt from eroding further. Because the key problem with inert anodes is anode corrosion, so we consider the optimal amount of MnO_2 is 1.0%.