gebhart/patrepeval huggingface dataset:
Ethidium binding sites on plasmid DNA determined by photoaffinity labeling. (1984)

Photoaffinity labeling of pBR322 with ethidium monoazide (8-azido-3-amino-5-ethyl-6-phenylphenanthridinium chloride) was used to provide evidence for the sequence specificity of ethidium binding to native DNA. DNA-drug interactions were examined at concentrations of eight covalently bound ethidium drugs per molecule of pBR322 (4363 base pairs). Restriction enzyme cutting was blocked by the covalent binding of a drug molecule at (or near) the enzyme recognition sequence. This phenomenon was observed with all restriction enzymes tested and was not limited to specific regions of the pBR322 molecule. Double-digestion experiments indicated that a drug molecule may bind 2 to 3 base pairs outside the recognition sequence and still block restriction enzyme digestion. Intact plasmid was treated with [3H]ethidium monoazide and digested with restriction enzymes. The amount of covalently-linked ethidium analog was quantitated for different restriction fragments and the G-C content of each fragment was determined from the DNA sequence. In approximately half of the fragments the drug appeared to preferentially bind at a G-C base pair. However, no preference for specific sequences such as 5'-C-G-3' was detected, as had been suggested by previous modeling studies with ethidium bromide. The other fragments were located in specific map regions of the plasmid and did not bind drug with a strict dependence on GC content suggesting that binding specificity may depend on more than one structural feature of the DNA.
Soluble nickel inhibits HIF‐prolyl‐hydroxylases creating persistent hypoxic signaling in A549 cells (2006)

Soluble nickel compounds are carcinogenic to humans although the mechanism by which they cause cancer remains unclear. One major consequence of exposure to nickel is the stabilization of hypoxia inducible factor-1alpha (HIF-1alpha), a protein known to be overexpressed in a variety of cancers. In this study, we report a persistent stabilization of HIF-1alpha by nickel chloride up to 72 h after the removal of nickel from the culture media. In addition, we show that the HIF-prolyl hydroxylases (PHD's) are inhibited when cells are exposed to nickel and that they remain repressed for up to 72 h after nickel is removed. We then show that nickel can inhibit purified HIF-PHD's 2 in vitro, through direct interference with the enzyme. Through theoretical calculations, we also demonstrate that nickel may be able to replace the iron in the active site of this enzyme, providing a plausible mechanism for the persistent inhibition of HIF-PHD's by nickel. The data presented suggest that nickel can interfere with HIF-PHD directly and does not inhibit the enzyme by simply depleting cellular factors, such as iron or ascorbic acid. Understanding the mechanisms by which nickel can inhibit HIF-PHD's and stabilize HIF-1alpha may be important in the treatment of cancer and ischemic diseases.
3D Deeply Supervised Network for Automatic Liver Segmentation from CT Volumes (2016)

Automatic liver segmentation from CT volumes is a crucial prerequisite yet challenging task for computer-aided hepatic disease diagnosis and treatment. In this paper, we present a novel 3D deeply supervised network (3D DSN) to address this challenging task. The proposed 3D DSN takes advantage of a fully convolutional architecture which performs efficient end-to-end learning and inference. More importantly, we introduce a deep supervision mechanism during the learning process to combat potential optimization difficulties, and thus the model can acquire a much faster convergence rate and more powerful discrimination capability. On top of the high-quality score map produced by the 3D DSN, a conditional random field model is further employed to obtain refined segmentation results. We evaluated our framework on the public MICCAI-SLiver07 dataset. Extensive experiments demonstrated that our method achieves competitive segmentation results to state-of-the-art approaches with a much faster processing speed.
Carthami flos extract and its component, stigmasterol, inhibit tumour promotion in mouse skin two‐stage carcinogenesis (1994)

Abstract An antitumour‐promoting activity in two‐stage carcinogenesis, is found in the methanol extract of the Carthami Flos ( Carthamus tinctorius L.; Compositae), which is a traditional Chinese medicine and natural pigment of rouge additivies in certain Asian countries. From these active fractions, δ 5 ‐ and δ 7 ‐sterol fractions were separated. The separation was examined for inhibitory activity against TPA‐induced inflammatory ear oedema in mice. Stigmasterol (71% in the mixture) was the most abundant of 14 sterols identified in the δ 5 ‐sterol fraction. Schottenol (70% in the mixture) constituted the dominant sterol of the δ 7 ‐sterol fraction. Furthermore, stigmasterol markedly inhibited tumour promotion in two‐stage carcinogenesis experiments.
The Chemical Basis of Fungal Bioluminescence (2015)

Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins. Furthermore, it was shown that 3-hydroxyhispidin leads to bioluminescence in extracts from four diverse genera of luminous fungi, thus suggesting a common biochemical mechanism for fungal bioluminescence.
Electrophilic reactions at single bonds. IX. Intermolecular hydrogen exchange and alkylation (alkylolysis) of alkanes with alkylcarbenium fluoroantimonates (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTElectrophilic reactions at single bonds. IX. Intermolecular hydrogen exchange and alkylation (alkylolysis) of alkanes with alkylcarbenium fluoroantimonatesGeorge A. Olah, Y. K. Mo, and Judith A. OlahCite this: J. Am. Chem. Soc. 1973, 95, 15, 4939–4951Publication Date (Print):July 1, 1973Publication History Published online1 May 2002Published inissue 1 July 1973https://pubs.acs.org/doi/10.1021/ja00796a028https://doi.org/10.1021/ja00796a028research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A new view for power system engineers (2003)

Integrated action graphics and integrated performance views, two concepts for simplifying a user's ability to evaluate a greater number of design options for large industrial facilities and for providing an enhanced understanding of the transient and steady state performance of power systems, are described. The traditional power system study, the traditional user-program interface, and the limitations of each are discussed. It is suggested that power engineers familiar with conventional system study techniques will readily adapt to the interactive-graphical method of analysis with minimum effort. Operating and plant engineers may find the technique invaluable in the study of system disturbances, for increasing operational efficiency, and for better preparing operators for critical emergencies. The advanced mathematical techniques used in this program make it an effective tool for the study of large power systems on a personal computer.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Principal components array processing for swath acoustic mapping (2002)

The authors propose a new swath mapping sonar design in which the number of concurrent backscatter angles-of-arrival (AOA) is extended beyond the existing limit of one currently afforded by swath bathymetry sidescan (SBS). The AOA estimation method, called "principal components decomposition" (PCD) utilizes an N element sidescan transducer array to estimate, in a total least squares sense over one or more samples, the principal component directions and associated intensities of the received backscatter at each range. PCD affords separation of as many as N-1 concurrent uncorrelated AOAs or N/2 coherent AOAs and unlike conventional beamforming has a resolution limit that is governed by the signal-to-noise ratio rather than the size of the array aperture. This paper presents a synopsis of the theory behind principal components simulated and experimental results for a corner reflector backscattering geometry imaged (mapped) using a six element prototype sonar.
Hermetic thin film encapsulation of mechanical transducers for smart label applications (2009)

For realizing an active smart RFID label monitoring mechanical shock and inclination during transport processes, a capacitive inertial sensor has been developed. Hermetic sealing of the microstructures has been done by a wafer level thin film encapsulation technology based on CF-polymer as sacrificial layer material and a stress-optimized dielectric layer stack as capping material. Long term stability of the thin film encapsulation hermeticity in dependence of the material stack was tested by measuring the pressure inside the package using the pressure dependency of viscous damping between the electrode plates. For the used material stacks containing SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> (H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</sub> ) or SiO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> (H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</sub> )/SiN <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">x</sub> (H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">y</sub> ) and sputtered aluminum a sufficient hermeticity with leak rates of 2E-10 Pamiddotl/s and 6E-12 Pamiddotl/s, respectively, could be achieved.
Differential twinax cable modeling by measured 4-port s-parameters (2006)

A method for making SPICE circuit model of differential twinax cable from measured 4-port S-parameters is proposed. Ideal transmission lines are used in the middle of the model to represent the major part of the cable delay. Commercial SPICE model generator based on S-parameters can be used for the rest parts of the model. The circuit models have good correlation with the original measured frequency-domain responses and can be used in any SPICE-like transient circuit simulation tools.
Photoluminescence study of MBE grown InGaN with intentional indium segregation (2005)

Proper control of MBE growth conditions has yielded an In0.13Ga0.87N thin film sample with emission consistent with In-segregation. The photoluminescence (PL) from this epilayer showed multiple emission components. Moreover, temperature and power dependent studies of the PL demonstrated that two of the components were excitonic in nature and consistent with indium phase separation. At 15 K, time resolved PL showed a non-exponential PL decay that was well fitted with the stretched exponential solution expected for disordered systems. Consistent with the assumed carrier hopping mechanism of this model, the effective lifetime, τ, and the stretched exponential parameter, β, decrease with increasing emission energy. Finally, room temperature micro-PL using a confocal microscope showed spatial clustering of low energy emission. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Stacked polymer waveguide type fan‐in/fan‐out device for dense multi‐core fibre (2015)

A compact fan‐in/fan‐out waveguide device for 19‐core uncoupled multi‐core fibre (MCF) was demonstrated using laminated polymer waveguide. The core was made of poly‐methyl‐methacrylate and the cladding was made of ultra‐violet (UV) cured epoxy resin which is solvent‐free oligomer. Since the oligomer does not shrink after UV‐curing, the surface of the cladding over the core ridge was flattened and the multiple layers can be laminated. The accurate core alignment in the vertical direction was realised by controlling the spin‐coat condition of cladding material precisely. The insertion loss from a 19‐core fibre to 19 single‐core fibres was measured to be <20 dB and the sum of the cross‐talk from all neighbouring cores was lower than −40 dB. The coupling loss which is caused by the offset of the core position and the spot size mismatch at the facet between the MCF core and the waveguide was <1.8 dB. It was shown that the coupling loss and the insertion loss can be improved by adopting a properly selected pair of polymer materials with appropriate value of index contrast and low absorption loss.
Development of Automotive Liquid Hydrogen Storage Systems (2004)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation G. Krainz, G. Bartlok, P. Bodner, P. Casapicola, Ch. Doeller, F. Hofmeister, E. Neubacher, A. Zieger; Development of Automotive Liquid Hydrogen Storage Systems. AIP Conf. Proc. 23 June 2004; 710 (1): 35–40. https://doi.org/10.1063/1.1774664 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
WB2838 (3-chloro-4-(2-amino-3-chlorophenyl)-pyrrole): Non-steroidal androgen-receptor antagonist produced by a Pseudomonas. (1993)

In the course of our search for non-steroidal androgen-receptor antagonists of microbial origin, Pseudomonas sp. No. 2838 was found to produce an inhibitor of androgen binding to its receptor. This compound, named WB2838, was isolated and identified as 3-chloro-4-(2-amino-3-chlorophenyl)-pyrrole. The IC50 value of WB2838 for partially purified rat prostate cytosol receptor was 8.0 x 10(-7) M. However, the IC50 value of WB2838 against estrogen-receptor binding was about 90-fold greater than that against androgen-receptor binding. WB2838 inhibited the growth of androgen-responsive mouse mammary carcinoma SC-3 cells in the presence of 10(-8) M testosterone at IC50 value of 4.1 x 10(-7) M. This inhibition was reversed by adding 10(-5) M testosterone to the culture medium. WB2838 also showed the inhibitory activity against the growth of the ventral prostate induced by testosterone propionate in castrated immature rats. Therefore, it was concluded that WB2838 was a non-steroidal androgen-receptor antagonist.
Dioxygen affinities of synthetic cobalt Schiff base complexes (1987)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDioxygen affinities of synthetic cobalt Schiff base complexesDian Chen and A. E. MartellCite this: Inorg. Chem. 1987, 26, 7, 1026–1030Publication Date (Print):April 1, 1987Publication History Published online1 May 2002Published inissue 1 April 1987https://pubs.acs.org/doi/10.1021/ic00254a013https://doi.org/10.1021/ic00254a013research-articleACS PublicationsRequest reuse permissionsArticle Views2585Altmetric-Citations235LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
A New Polyvalent Pseudomonas Vaccine (1977)

This paper describes the preparation, chemical characterisation and immunogenic properties of a new polyvalent pseudomonas vaccine. New cultural methods were devised which allowed a build-up of the immunogen in the cell wall of the bacteria, and mild extraction techniques were used to remove the immunogens from the cell wall of living bacteria without apparent physical or chemical change which might affect immunogenicity. The polyvalent vaccine comprised 16 component vaccines, each a lipid-protein-carbohydrate complex extracted from one of the 16 different serotypes of Pseudomonas aeruginosa. Single injections into mice of each of the 16 component vaccines induced protection against several strains of homologous serotypes within 3 days of vaccination. The polyvalent vaccine induced similar protection against several strains of each of the 16 serotypes. We would like to thank R. E. Dyster, K. Digby and J. Simmonds for their technical assistance.
Analysis of unslotted direct-sequence spread spectrummultiple access network with packet combining (1997)

A packet combining scheme is investigated for an unslotted random access code division multiple access network over a frequency selective Rayleigh fading channel. It is shown that this simple diversity combining mechanism is highly advantageous for systems which can both tolerate a certain delay and operate over highly time-varying channels.
Optimal Layout of CMOS Functional Arrays (1981)

Designers of MOS LSI circuits can take advantage of complex functional cells in order to achieve better performance. This paper discusses the implementation of a random logic function on an array of CMOS transistors. A graph-theoretical algorithm which minimizes the size of an array is presented. This method is useful for the design of cells used in conventional design automation systems.
The Rv1712 Locus from <i>Mycobacterium tuberculosis</i> H37Rv Codes for a Functional CMP Kinase That Preferentially Phosphorylates dCMP (2009)

The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed.
PAINTS & COATINGS (1996)

RETURN TO ISSUEPREVNewsNEXTPAINTS & COATINGSMARC S. REISCHCite this: Chem. Eng. News 1996, 74, 42, 44–67Publication Date (Print):October 14, 1996Publication History Published online12 November 2010Published inissue 14 October 1996https://pubs.acs.org/doi/10.1021/cen-v074n042.p044https://doi.org/10.1021/cen-v074n042.p044newsACS PublicationsCopyright © 1996 AMERICAN CHEMICAL SOCIETYArticle Views16Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Coating materials,Color,Industrial manufacturing Get e-Alerts
Human lactoferrin binding in clinical isolates of Staphylococcus aureus (1991)

Human lactoferrin (HLf) is an iron-binding protein and a host-defence component at the mucosal surface. Recently, a specific receptor for HLf has been identified on a strain of Staphylococcus aureus associated with toxic shock syndrome. We have looked for the occurrence of 125I-HLf binding among 489 strains of S. aureus isolated from various clinical sources. HLf binding was common among S. aureus strains associated with furunculosis (94.3%), toxic shock syndrome (94.3%), endocarditis (83.3%) and septicaemia (82.8%) and other (nasal, vaginal or ocular) infections (96.1%) with a mean binding (in fmol) of 29.1, 21.9, 16.9, 22.2 and 29.2 respectively; the differences between mean HLf binding values of 29.1-29.2, 21.9-22.2 and 16.9 were significant. Furunculosis-associated (low-invasive or localised) isolates were high-to-moderate binders of HLf; 50% gave positive results at a threshold of greater than 31 fmol of 125I-HLf bound. In contrast, endocarditis-associated (high-invasive or systemic) isolates demonstrated low binding and did not bind 125I-HLf at the above threshold level. S. aureus recognised human or bovine Lf. However, bound 125I-HLf was more effectively inhibited in a dose-dependent manner by unlabelled bovine Lf than by homologous HLf. Binding of 125I-HLf to staphylococci was optimal with organisms grown in agar compared with those from broth cultures. The binding capacity of S. aureus was abolished when strains were grown on carbohydrate- and salt-rich agar media. HLf-binding ability of S. aureus did not correlate with fibronectin, fibrinogen, immunoglobulin G or laminin binding.
Mechanisms of Ca<sup>2+</sup> Overload in Reperfused Ischemic Myocardium (1990)

Macrophage polarization refers to how macrophages have been activated at a given point in space and time. Polarization is not fixed, as macrophages are sufficiently plastic to integrate multiple signals, such as those from microbes, damaged tissues, and ...Read More
On the solubilization properties of surfactant-polymer complexes. (1976)

Polyethylene glycol (PEG) and polyvinylpyrrolidone (PVP) affect the solubilization properties of sodium dodecyl sulphate (SDS). When these polymers are added to SDS solution, its solubilization power for riboflavin, khellin and propyl p-hydroxybenzoate is markedly decreased. In case of riboflavin and khellin, the polymer effect is more pronounced at high surfactant concentrations; for propyl p-hydroxybenzoate, the polymer effect is inversely proportional to the surfactant concentration. On the other hand, the solubilization power of SDS towards methyl and butyl p-hydroxybenzoate is greatly enhanced by PEG or PVP. In case of methyl p-hydroxybenzoate the effect of PEG is more pronounced at high surfactant concentrations; the effect of PVP is not significantly dependent on the surfactant concentration. For butyl p-hydroxybenzoate, the polymer effect decreases markedly with increasing surfactant concentration.