gebhart/patrepeval huggingface dataset:
Molecular genetic responses to lysergic acid diethylamide include transcriptional activation of <i>MAP kinase phosphatase‐1, C/EBP‐β</i> and <i>ILAD‐1</i>, a novel gene with homology to arrestins (2004)

We recently demonstrated that the potent hallucinogenic drug lysergic acid diethylamide (LSD) dynamically influences the expression of a small collection of genes within the mammalian prefrontal cortex. Towards generating a greater understanding of the molecular genetic effects of hallucinogens and how they may relate to alterations in behavior, we have identified and characterized expression patterns of a new collection of three genes increased in expression by acute LSD administration. These genes were identified through additional screens of Affymetrix DNA microarrays and examined in experiments to assess dose-response, time course and the receptor mediating the expression changes. The first induced gene, C/EBP-beta, is a transcription factor. The second gene, MKP-1, suggests that LSD activates the MAP (mitogen activated protein) kinase pathway. The third gene, ILAD-1, demonstrates sequence similarity to the arrestins. The increase in expression of each gene was partially mediated through LSD interactions at 5-HT2A (serotonin) receptors. There is evidence of alternative splicing at the ILAD-1 locus. Furthermore, data suggests that various splice isoforms of ILAD-1 respond differently at the transcriptional level to LSD. The genes thus far found to be responsive to LSD are beginning to give a more complete picture of the complex intracellular events initiated by hallucinogens.
Classical simulation of noncontextual Pauli Hamiltonians (2020)

Noncontextual Pauli Hamiltonians decompose into sets of Pauli terms to which joint values may be assigned without contradiction. We construct a quasiquantized model for noncontextual Pauli Hamiltonians. Using this model, we give an algorithm to classically simulate the noncontextual variational quantum eigensolver. We also use the model to show that the noncontextual Hamiltonian problem is NP-complete. Finally, we explore the applicability of our quasiquantized model as an approximate simulation tool for contextual Hamiltonians. These results support the notion of noncontextuality as classicality in near-term quantum algorithms.
Vitamin E and Glutathione are Required for Preservation of Microsomal Glutathione S‐Transferase from Oxidative Stress in Microsomes (1990)

Glutathione (GSH) inhibited lipid peroxidation induced by NADPH-BrCCl3 in vitamin E sufficient microsomes, but did not in phenobarbital (PB)-treated microsomes (containing about 60% of normal vitamin E) or in vitamin E-deficient microsomes (containing about 30% of normal vitamin E). There was a good correlation between the increased formation of CHCl3 from BrCCl3 in the presence of GSH under anaerobic conditions and the vitamin E level in the microsomes. A normal level of vitamin E in microsomes was thus very important for GSH-dependent inhibition of lipid peroxidation and for the efficient formation of CHCl3 from BrCCl3. Bromosulfophthalein (BSP) eliminated the effects of GSH on lipid peroxidation and CHCl3 formation. The apparent Km and Vmax of substrates for GSH S-transferase were changed by in vivo depletion of vitamin E in microsomes, and the Vmax/Km values were significantly reduced. The enzyme activity in microsomes was inactivated following the loss of vitamin E during in vitro lipid peroxidation, and GSH prevented the loss of vitamin E and protected the enzyme from attack by free radicals. GSH inhibited lipid peroxidation induced by NADPH-Fe2+ and the loss of GSH S-transferase activity during the peroxidation in PB-treated microsomes, but did not in the case of induction by NADPH-BrCCl3. A possible relation between the microsomal GSH S-transferase activity and defense by GSH against lipid peroxidation in microsomes is discussed.
SAW filters using ZnO thin films (2003)

Surface acoustic wave (SAW) filters using ZnO thin films are discussed. These films have a low dielectric constant and low temperature coefficients, but stability and producibility of the films are a concern. Some fabrication techniques for the films are described, and packaging and reliability of the filters are discussed. SAW filters with 1.5 to 2 GHz center frequency are reported.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
“Amar a Igreja é fazê-la arder”: Três rumos na Igreja para o pós-pandemia (2020)

Tres possiveis cenarios se descortinam no pos-pandemia para a pastoral da Igreja catolica: 1. o dos grupos tradicionalistas, que sao refratarios ao dialogo com o mundo contemporâneo, que nao aceitam a reforma do papa Francisco; 2. o da maioria do corpo eclesial, que podera mudar seus rumos, tendo aprendido com o que viveu durante a pandemia; 3. o de grupos que buscam avancar ainda mais no caminho tracado por Jesus de Nazare, aprofundando a descoberta de Deus como misericordia, que busca acender as brasas de uma Igreja da misericordia.
Equivalence between Two-Qubit Entanglement and Secure Key Distribution (2003)

We study the problem of secret key distillation from bipartite states in the scenario where Alice and Bob can perform measurements only at the single-copy level and classically process the obtained outcomes. Even with these limitations, secret bits can be asymptotically distilled by the honest parties from any two-qubit entangled state, under any individual attack. Our results point out a complete equivalence between two-qubit entanglement and secure key distribution: a key can be established through a one-qubit channel if and only if it allows one to distribute entanglement. These results can be generalized to a higher dimension for all those states that are one-copy distillable.
Determination of absolute amounts of GDP and GTP bound to Ras in mammalian cells: comparison of parental and Ras-overproducing NIH 3T3 fibroblasts. (1995)

We devised enzyme-based methods to measure fmol amounts of GDP and GTP and applied these methods to measure absolute amounts of Ras-bound GDP and GTP in NIH 3T3 fibroblasts. We found that parental NIH 3T3 cells contained 509 and 1.3 fmol of Ras-bound GDP and GTP, respectively, per mg of cellular protein and that stable transfectants of NIH 3T3 cells overexpressing wild-type Ha-Ras contained 7008 and 21.3 fmol of Ras-bound GDP and GTP, respectively, per mg of cellular protein; thus, in both cell types < 0.3% of Ras was in the active GTP-bound state. In contrast, NIH 3T3 cells overexpressing an activated form of Ha-Ras contained 5013 and 2049 fmol of Ras-bound GDP and GTP, respectively, per mg of protein, yielding 29% of Ras in the GTP-bound state. Since intracellular Ras is probably all in a guanine-nucleotide bound state, this method allows one to calculate the number of Ras molecules in each cell: parental NIH 3T3 cells and the Ha-Ras overproducing cells contain approximately 20,000 and approximately 275,000 Ras molecules per cell, respectively. We also incubated parental NIH 3T3 cells with 32PO4 and determined the radioactivity in Ras-bound GDP and GTP and the specific activity of cytosolic GDP and GTP; these experiments indicated that Ras-bound GTP may not be in equilibrium with the total cytosolic GTP pool.
Influenza A virus uses the aggresome processing machinery for host cell entry (2014)

During cell entry, capsids of incoming influenza A viruses (IAVs) must be uncoated before viral ribonucleoproteins (vRNPs) can enter the nucleus for replication. After hemagglutinin-mediated membrane fusion in late endocytic vacuoles, the vRNPs and the matrix proteins dissociate from each other and disperse within the cytosol. Here, we found that for capsid disassembly, IAV takes advantage of the host cell's aggresome formation and disassembly machinery. The capsids mimicked misfolded protein aggregates by carrying unanchored ubiquitin chains that activated a histone deacetylase 6 (HDAC6)-dependent pathway. The ubiquitin-binding domain was essential for recruitment of HDAC6 to viral fusion sites and for efficient uncoating and infection. That other components of the aggresome processing machinery, including dynein, dynactin, and myosin II, were also required suggested that physical forces generated by microtubule- and actin-associated motors are essential for IAV entry.
Efficient in-memory data management (2014)

This paper analyzes the performance of three systems for in-memory data management: Memcached, Redis and the Resilient Distributed Datasets (RDD) implemented by Spark. By performing a thorough performance analysis of both analytics operations and fine-grained object operations such as set/get , we show that neither system handles efficiently both types of workloads. For Memcached and Redis the CPU and I/O performance of the TCP stack are the bottlenecks -- even when serving in-memory objects within a single server node. RDD does not support efficient get operation for random objects, due to a large startup cost of the get job. Our analysis reveals a set of features that a system must support in order to achieve efficient in-memory data management.
Pace condition detection for debugging shared-memory parallel programs (1992)

This thesis addresses theoretical and practical aspects of the dynamic detecting and debugging of race conditions in shared-memory parallel programs. To reason about race conditions, we present a formal model that characterizes actual, observed, and potential behaviors of the program. The actual behavior precisely represents the program execution, the observed behavior represents partial information that can be reasonably recorded, and the potential behavior represents alternate executions possibly allowed by nondeterministic timing variations. These behaviors are used to characterize different types of race conditions, general races and data races, which pertain to different classes of parallel programs and require different detection techniques. General races apply to programs intended to be deterministic; data races apply to nondeterministic programs containing critical sections.
We prove that, for executions using synchronization powerful enough to implement two-process mutual exclusion, locating every general race or data race is an NP-hard problem. However, for data races, we show that detecting only a subset of all races is sufficient for debugging. We also prove that, for weaker types of synchronization, races can be efficiently located.
We present post-mortem algorithms for detecting race conditions as accurately as possible, given the constraint of limited run-time information. We characterize those races that are direct manifestations of bugs and not artifacts caused by other races, imprecise run-time traces (causing false races to appear real), or unintentional synchronization (caused by shared-memory references). Our techniques analyze the observed behavior to conservatively locate races that either did occur or had the potential of occurring, and that were unaffected by any other race in the execution.
Finally, we describe a prototype data race detector that we used to analyze a sample collection of parallel programs. Experiments indicate that our techniques effectively pinpoint non-artifact races, directing the programmer to parts of the execution containing direct manifestations of bugs. In all programs analyzed, our techniques reduced hundreds to thousands of races down to four or fewer that required investigation.
Restricted Enzooticity of Hepatitis E Virus Genotypes 1 to 4 in the United States (2011)

Hepatitis E is recognized as a zoonosis, and swine are known reservoirs, but how broadly enzootic its causative agent, hepatitis E virus (HEV), is remains controversial. To determine the prevalence of HEV infection in animals, a serological assay with capability to detect anti-HEV-antibody across a wide variety of animal species was devised. Recombinant antigens comprising truncated capsid proteins generated from HEV-subgenomic constructs that represent all four viral genotypes were used to capture anti-HEV in the test sample and as an analyte reporter. To facilitate development and validation of the assay, serum samples were assembled from blood donors (n = 372), acute hepatitis E patients (n = 94), five laboratory animals (rhesus monkey, pig, New Zealand rabbit, Wistar rat, and BALB/c mouse) immunized with HEV antigens, and four pigs experimentally infected with HEV. The assay was then applied to 4,936 sera collected from 35 genera of animals that were wild, feral, domesticated, or otherwise held captive in the United States. Test positivity was determined in 457 samples (9.3%). These originated from: bison (3/65, 4.6%), cattle (174/1,156, 15%), dogs (2/212, 0.9%), Norway rats (2/318, 0.6%), farmed swine (267/648, 41.2%), and feral swine (9/306, 2.9%). Only the porcine samples yielded the highest reactivities. HEV RNA was amplified from one farmed pig and two feral pigs and characterized by nucleotide sequencing to belong to genotype 3. HEV infected farmed swine primarily, and the role of other animals as reservoirs of its zoonotic spread appears to be limited.
[Exon-intron structure of genes of fungi genomes]. (2009)

Objects of research--genes of A. fumigatus, C. glabrata, C. neoformans, D. hansenii, E. cuniculi, E. gossypii, K. lactis, M. grisea, N. crassa, S. cerevisiae, S. pombe, U. maydis and Y. lipolytica fungi genomes. Methods of research are computer calculation. The content of genes with exon-intron structure in fungi genomes are from 0.7 to 97.0%. The exon-intron gene structure was changed when the portion of genes with introns increased. In A. fumigatus, C. neoformans, M. grisea, N. crassa, S. pombe and U. maydis genomes that linear dependence between gene lengths, sum of exon lengths and intron number in genes was established.
Quantitative muscle strength assessment in duchenne muscular dystrophy: longitudinal study and correlation with functional measures (2012)

Our longitudinal data using QMT in a cohort of DMD patients suggest that this could be used as an additional tool to monitor changes, providing additional information on segmental strength.
Intense Natural Selection in a Population of Darwin's Finches (Geospizinae) in the Galápagos (1981)

Survival of Darwin's finches through a drought on Daphne Major Island was nonrandom. Large birds, especially males with large beaks, survived best because they were able to crack the large and hard seeds that predominated in the drought. Selection intensities, calculated by O'Donald's method, are the highest yet recorded for a vertebrate population.
Synthesis of fast response, temperature-sensitive poly(N-isopropylacrylamide) gel (1991)

A microporous gel of N-isopropylacrylamide was synthesized by increasing the solution temperature above the lower critical solution temperature of the polymer during the copolymerization/crosslinking reaction. In response to changes in temperature this gel expands 120 times faster and contrasts 3000 times faster than comparable homogeneous gels
Morphology Modulating the Wettability of a Diamond Film (2014)

Control of the wetting property of diamond surface has been a challenge because of its maximal hardness and good chemical inertness. In this work, the micro/nanoarray structures etched into diamond film surfaces by a maskless plasma method are shown to fix a surface's wettability characteristics, and this means that the change in morphology is able to modulate the wettability of a diamond film from weakly hydrophilic to either superhydrophilic or superhydrophobic. It can be seen that the etched diamond surface with a mushroom-shaped array is superhydrophobic following the Cassie mode, whereas the etched surface with nanocone arrays is superhydrophilic in accordance with the hemiwicking mechnism. In addition, the difference in cone densities of superhydrophilic nanocone surfaces has a significant effect on water spreading, which is mainly derived from different driving forces. This low-cost and convenient means of altering the wetting properties of diamond surfaces can be further applied to underlying wetting phenomena and expand the applications of diamond in various fields.
An AuNPs-functionalized AlGaN/GaN high electron mobility transistor sensor for ultrasensitive detection of TNT (2015)

In this work, we developed a gold nanoparticle functionalized AlGaN/GaN HEMT sensor for fast and ultrasensitive detection of TNT. This HEMT sensor showed attractive properties for TNT detection in terms of speed, sensitivity and miniaturization.
Modern management of premenstrual syndrome (1993)

Premenstrual syndrome (PMS), always a confusing and controversial area of medicine, now receives its due with this comprehensive monograph. Its basic assumption is that occurrences in the female reproductive endocrine system may trigger pre-existing factors, leading to the constellation of symptoms known as PMS. Diagnosis, often difficult to come by with PMS, is laid out simply and clearly. There are chapters on the interrelationship of medical illnesses and the menstrual cycle, on the parallels between PMS and psychiatric illnesses and on the physiology of mood.
Analysis of gap junction formation in rat hepatocytes by intravenous injection of an anti-connexin32 monoclonal antibody (HAM8) (2001)

HAM8 monoclonal antibody was used to examine the mechanism of connexin32 (Cx32) formation in the rat model in vivo by immunofluorescence and immunoelectron microscopy. After a single intravenous injection of HAM8 IgG, a number of HAM8 signals were observed at the sinusoidal face, between adjacent hepatocytes as well as in the cytoplasm stained with only 2nd fluorescent antibody. Moreover, the in vivo localization of the HAM8 antigen appeared to change with time. At 5 min after the antibody injection, Cx32 signals from liver sections were clearly detected at the sinusoidal face. Fifteen minutes later, numerous linear and dotted fluorescent signals were observed between hepatocytes; in addition, much punctate staining was found at the sinusoidal face and in hepatocytes. These findings were identified by immunoelectron microscopy. Interestingly, 1 hr later, much punctate staining considered to be similar to those seen in the normal rat liver tissues was observed between adjacent hepatocytes, suggesting that a great deal of Cx32 combined with HAM8 have been assembled into identifiable gap junction plaques. Five hours later, intercellular and intracellular Cx32 signals were infrequently detected. When staining was performed with HAM8 and 2nd antibody, however, numerous Cx32 signals were again observed between neighboring hepatocytes, as punctate staining appearing in a pattern approximately the same as that seen in the normal liver tissue. Based on these results, we assumed that a precursor gap was present during Cx32 formation, and discussed the pathways of Cx32 formation and the degradation of Cx32 as well as that of HAM8.
Assembly scaffold NifEN: A structural and functional homolog of the nitrogenase catalytic component (2016)

NifEN is a biosynthetic scaffold for the cofactor of Mo-nitrogenase (designated the M-cluster). Previous studies have revealed the sequence and structural homology between NifEN and NifDK, the catalytic component of nitrogenase. However, direct proof for the functional homology between the two proteins has remained elusive. Here we show that, upon maturation of a cofactor precursor (designated the L-cluster) on NifEN, the cluster species extracted from NifEN is spectroscopically equivalent and functionally interchangeable with the native M-cluster extracted from NifDK. Both extracted clusters display nearly indistinguishable EPR features, X-ray absorption spectroscopy/extended X-ray absorption fine structure (XAS/EXAFS) spectra and reconstitution activities, firmly establishing the M-cluster-bound NifEN (designated NifEN(M)) as the only protein other than NifDK to house the unique nitrogenase cofactor. Iron chelation experiments demonstrate a relocation of the cluster from the surface to its binding site within NifEN(M) upon maturation, which parallels the insertion of M-cluster into an analogous binding site in NifDK, whereas metal analyses suggest an asymmetric conformation of NifEN(M) with an M-cluster in one αβ-half and an empty cluster-binding site in the other αβ-half, which led to the proposal of a stepwise assembly mechanism of the M-cluster in the two αβ-dimers of NifEN. Perhaps most importantly, NifEN(M) displays comparable ATP-independent substrate-reducing profiles to those of NifDK, which establishes the M-cluster-bound αβ-dimer of NifEN(M) as a structural and functional mimic of one catalytic αβ-half of NifDK while suggesting the potential of this protein as a useful tool for further investigations of the mechanistic details of nitrogenase.
Insecticide Residues in Urine, Determination of Urinary p-Nitrophenol by Thin-Layer Chromatography and Phosphorimetry (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInsecticide Residues in Urine, Determination of Urinary p-Nitrophenol by Thin-Layer Chromatography and PhosphorimetryH. A. Moye and J. D. WinefordnerCite this: J. Agric. Food Chem. 1965, 13, 6, 533–536Publication Date (Print):November 1, 1965Publication History Published online1 May 2002Published inissue 1 November 1965https://pubs.acs.org/doi/10.1021/jf60142a013https://doi.org/10.1021/jf60142a013research-articleACS PublicationsRequest reuse permissionsArticle Views60Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
TUCUXI: The InTelligent Hunter Agent for Concept Understanding and LeXical ChaIning (2005)

In this paper we present TUCUXI, an intelligent hunter agent that replaces traditional keywords-based queries on the Web with a user-provided domain ontology, where meanings to be searched are not ambiguous. TUCUXI judges the relevance of the retrieved pages by matching the domain ontology against a simplified, but semantically rich, document representation (Map of Meanings). The Map of Meanings extraction involves the Lexical Chaining technique, from the Natural Language Processing (NLP) research field.
Low-noise readout using active reset for CMOS APS (2000)

Pixel reset noise sets the fundamental detection limit on photodiode based CMOS image sensors. Reset noise in standard active pixel sensor (APS) is well understood and is of order kT/C. In this paper we present a new technique for resetting photodiodes, called active reset, which reduces reset noise without adding lag. Active reset can be applied to standard APS. Active reset uses bandlimiting and capacitive feedback to reduce reset noise. This paper discusses the operation of an active reset pixel, and presents an analysis of lag and noise. Measured results from a 6 transistor per pixel 0.35 micrometers CMOS implementation are presented. Measured results show that reset noise can be reduced to less than kT/18C using active reset. We find that theory simulation and measured results all match closely.