gebhart/patrepeval huggingface dataset:
Curcuminoids Form Reactive Glucuronides In Vitro (2006)

Curcumin is of current interest because of its putative anti-inflammatory, anticarcinogenic, and anti-Alzheimer's activity, but its pharmacokinetic and metabolic fate is poorly understood. The present in vitro study has therefore been conducted on the glucuronidation of curcumin and its major phase I metabolite, hexahydro-curcumin, as well as of various natural and artificial analogs. The predominant glucuronide generated by rat and human liver microsomes from curcumin, hexahydro-curcumin, and other analogs with a phenolic hydroxyl group was a phenolic glucuronide according to LC-MS/MS analysis. However, a second glucuronide carrying the glucuronic acid moiety at the alcoholic hydroxyl group was formed from the same curcuminoids, but not hexahydro-curcuminoids, by human microsomes. Curcuminoids without a phenolic hydroxyl group gave rise to the aliphatic glucuronide only. The phenolic glucuronides of curcuminoids, but not of hexahydro-curcuminoids, were rather lipophilic and, in part, unstable in aqueous solution, their stability depending strongly on the type of aromatic substitution. The phenolic glucuronide of curcumin and of its natural congeners, but not the parent compounds, clearly inhibited the assembly of microtubule proteins under cell-free conditions, implying chemical reactivity of the glucuronides. These novel properties of the major phase II metabolites of curcuminoids deserve further investigation.
Metrology of laser-produced plasma light source for EUV lithography (2005)

Metrology concepts and related results are discussed for characterization of extreme ultraviolet (EUV) light sources based on laser-produced plasmas using metal foil and droplet targets. Specific designs of narrow-band EUV detectors employing multilayer mirrors and broadband detectors for droplet steering are described. Spatially resolved plasma imaging using in-band EUV pinhole cameras is discussed. A grazing-incidence flat-field EUV spectrometer is described that has been employed for spectroscopy in the 6 nm - 22 nm range. In addition, spectroscopic data of out-of-band radiation in the ultraviolet and visible spectral regions are presented. Results obtained for different wavelengths of the incident laser radiation and for both tin- and lithium foil- and droplet- targets are discussed.
P2570Synergistic activation of the cardiac enhancer landscape during reprogramming (2019)

Abstract Introduction Cardiogenic transcription factors (TFs), Gata4, Mef2c, and Tbx5, can directly reprogram fibroblasts to a cardiac fate and their cardiogenic activity is enhanced by the Hand2 TF and the Akt1 kinase. Although these cardiac reprogramming factors are key regulators of heart development, their expression and biological functions are not limited to the heart, and the mechanism by which these cardiac factors orchestrate reprogramming remains unclear. Purpose We sought to study the molecular mechanisms by which cardiac reprogramming factors contribute to cell fate conversion using a genome-wide approach. Methods Here, we used chromatin immunoprecipitation followed by massively parallel DNA sequencing (ChIP-seq) to explore the genomic binding sites of reprogramming TFs and the landscape of active enhancers, annotated by H3K27ac histone modification, during cardiac reprogramming. Results We found that reprogramming TFs rapidly silence fibroblast enhancers and synergistically activate cardiac enhancers which were predominantly enriched with Mef2 motifs. Addition of Hand2 and Akt1 to GMT expands TF co-occupancy and thereby activates additional cardiac enhancers, which further augments cardiac gene expression. Moreover, additional cardiac enhancers were sequentially activated during the reprogramming process, in accordance with the temporal acquisition of functional phenotypes in induced cardiac-like myocytes. We discovered that subsets of reprogramming enhancers are conserved among other cardiogenic processes, and a collection of reprogramming enhancers displayed unique spatial expression patterns in the developing heart in transgenic mouse embryos. Conclusion Our study describes the epigenomic dynamics that underlie cardiac reprogramming which is cooperatively orchestrated by reprogramming factors to convert fibroblasts toward a cardiac lineage. Acknowledgement/Funding NIH (AR-067294, HL-130253, HL-138426, HD-087351), Fondation Leducq Transatlantic Networks of Excellence in Cardiovascular Research
An Efficient WS-QoS Broker Based Architecture for Web Services Selection (2010)

Web Service selection is a key component in service-oriented computing. Service-oriented Architectures follow the find-bindexecute paradigm in which service providers register their services in public or private registries, which clients use to locate web services. The QoS based web service selection mechanisms plays an essential role in service-oriented architectures, because most of the applications want to use services that accurately meet their requirements. Currently, the UDDI catalogue supports only primitive matching mechanisms and provides no control over the quality of registered services. We propose a QoS broker based architecture for dynamic web service selection which facilitates the clients to specify the non-functional requirements like QoS along with functional requirements. The paper presents an efficient mechanism for finding the most suitable web service according to the consumer’s requirements.
“Double Click” Reaction on 7-Deazaguanine DNA: Synthesis and Excimer Fluorescence of Nucleosides and Oligonucleotides with Branched Side Chains Decorated with Proximal Pyrenes (2009)

The 7-tripropargylamine-7-deaza-2'-deoxyguanosine (2) containing two terminal triple bonds in the side chain was synthesized by the Sonogashira cross-coupling reaction from the corresponding 7-iodo nucleoside 1b. This was protected at the 2-amino group with an iso-butyryl residue, affording the protected intermediate 5. Then, compound 5 was converted to the 5'-O-DMT derivative 6, which on phosphitylation afforded the phosphoramidite 7. This was employed in solid-phase synthesis of a series of oligonucleotides. T(m) measurements demonstrate that a covalently attached tripropargylamine side chain increases duplex stability. Both terminal triple bonds of nucleoside 2 and corresponding oligonucleotides were functionalized by the Cu(I)-mediated 1,3-dipolar cycloaddition "double click reaction" with 1-azidomethyl pyrene 3, decorating the side chain with two proximal pyrenes. While the monomeric tripropargylamine nucleoside with two proximal pyrenes (4) shows strong excimer fluorescence, the ss-oligonucleotide containing 4 does not. This was also observed for ds-oligonucleotides when the complementary strand was unmodified. However, duplex DNA bearing pyrene residues in both strands exhibits strong excimer fluorescence when each strand contains two pyrene residues linked to the tripropargylamine moiety. This pyrene-pyrene interstrand interaction occurs when the pyrene modification sites of the duplex are separated by two base pairs which bring the fluorescent dyes in a proximal position. Molecular modeling indicates that only two out of four pyrene residues are interacting forming the exciplex while the other two do not communicate.
MASHaBLE (2016)

We present new applications for cryptographic secret handshakes between mobile devices on top of Bluetooth Low-Energy (LE). Secret handshakes enable mutual authentication, with the property that the parties learn nothing about each other unless they have been both issued credentials by a group administrator. This property provides strong privacy guarantees that enable interesting applications. One of them is proximity-based discovery for private communities. We introduce MASHaBLE, a mobile application that enables participants to discover and interact with nearby users if and only if they belong to the same secret community. We use direct peer-to-peer communication over Bluetooth LE, rather than relying on a central server. We discuss the specifics of implementing secret handshakes over Bluetooth LE and present our prototype implementation.
Morphology of Skin Microvasculature in Psoriasis (1989)

To establish the character of microvascular changes in psoriatic skin and their specificity, 29 skin biopsies of psoriatics (20 in exacerbation and 9 in a stationary stage) were investigated using histologic, histochemical, immunomorphologic, electron-microscopic, and morphometric methods. Five biopsies of uninvolved skin in scleroderma and five of diabetes mellitus patients were studied with the same technique for comparison. The results showed that structural changes depend on disease stage and the clinical appearance of lesions. Microvascular changes precede papule appearance during exacerbation and gradually increase during papule development. They comprise vascular dilatation, bridged fenestrations and gaps in endothelium, edematous areas in the cytoplasm of endotheliocytes, myocytes and pericytes, basement-membrane-zone thickening, and cell extravasation--signs of increased vascular permeability. Immunoglobulin G deposits in vascular walls, degranulation of mast cells, and extravasation of lymphocytes and neutrophils indicate that inflammation is a basic process during exacerbation and that immune mechanisms play an important role in the pathogenesis of inflammation. Microvascular changes in scleroderma and diabetes mellitus are different in nature and do not resemble those in psoriasis.
A Thermal Stabilizer for LiPF[sub 6]-Based Electrolytes of Li-Ion Cells (2002)

We report that tris(2,2,2-trifluoroethyl) phosphite (TTFP), in which the oxidization number of phosphor is three (III), can effectively improve thermal stability of -based electrolytes of Li-ion cells. Effect of TTFP on the performance of Li/graphite, Li/cathode, and Li-ion cells is estimated using cyclic voltammetry and galvanostatic cycling. Results show that TTFP is electrochemically stable in Li-ion chemistry. We also found that TTFP can prevent propylene carbonate (PC) decomposition and graphite exfoliation in Li/graphite half-cell. We propose a mechanism of TTFP protecting and discuss the effect of TTFP on cell performance. © 2002 The Electrochemical Society. All rights reserved.
Mannosylglycerate and Di- <i>myo</i> -Inositol Phosphate Have Interchangeable Roles during Adaptation of Pyrococcus furiosus to Heat Stress (2014)

Marine hyperthermophiles accumulate small organic compounds, known as compatible solutes, in response to supraoptimal temperatures or salinities. Pyrococcus furiosus is a hyperthermophilic archaeon that grows optimally at temperatures near 100°C. This organism accumulates mannosylglycerate (MG) and di-myo-inositol phosphate (DIP) in response to osmotic and heat stress, respectively. It has been assumed that MG and DIP are involved in cell protection; however, firm evidence for the roles of these solutes in stress adaptation is still missing, largely due to the lack of genetic tools to produce suitable mutants of hyperthermophiles. Recently, such tools were developed for P. furiosus, making this organism an ideal target for that purpose. In this work, genes coding for the synthases in the biosynthetic pathways of MG and DIP were deleted by double-crossover homologous recombination. The growth profiles and solute patterns of the two mutants and the parent strain were investigated under optimal growth conditions and also at supraoptimal temperatures and NaCl concentrations. DIP was a suitable replacement for MG during heat stress, but substitution of MG for DIP and aspartate led to less efficient growth under conditions of osmotic stress. The results suggest that the cascade of molecular events leading to MG synthesis is tuned for osmotic adjustment, while the machinery for induction of DIP synthesis responds to either stress agent. MG protects cells against heat as effectively as DIP, despite the finding that the amount of DIP consistently increases in response to heat stress in the nine (hyper)thermophiles examined thus far.
Identification of the Major Phosphorylation Sites for Protein Kinase C in Kit/Stem Cell Factor Receptor in Vitro and in Intact Cells (1995)

The c-kit-encoded tyrosine kinase receptor for stem cell factor (Kit/SCFR) is crucial for the development of hematopoietic cells, melanoblasts, and germ cells. Ligand stimulation of Kit/SCFR leads to receptor dimerization and autophosphorylation on tyrosine residues. We recently showed, that protein kinase C (PKC) acts in an SCF-stimulated negative feedback loop, which controls Kit/SCFR tyrosine kinase activity and modulates the cellular responses to SCF (Blume-Jensen, P., Siegbahn, A., Stabel, S., Heldin, C.-H., and Rönnstrand, L. (1993) EMBO J. 12, 4199-4209). We present here the identification of the major phosphorylation sites for PKC in Kit/SCFR. Two serine residues in the kinase insert, Ser-741 and Ser-746, are PKC-dependent phosphorylation sites in vivo and account for all phosphorylation by PKC in vitro. Together they comprise more than 60% of the total SCF-stimulated receptor phosphorylation in living cells and 85-90% of its phosphorylation in resting cells. Two additional serine residues, Ser-821 close to the major tyrosine autophosphorylation site in the kinase domain and Ser-959 in the carboxyl terminus are SCF-stimulated PKC-dependent phosphorylation sites. However, they are not phosphorylated directly by PKC-alpha in vitro. Both specific receptor tyrosine autophosphorylation and specific receptor-associated phosphatidylinositide 3'-kinase activity was increased approximately 2-fold in response to SCF in PAE cells stably expressing Kit/SCFR(S741A/S746A). Furthermore, the kinase activity of Kit/SCFR(S741A/S746A) toward an exogenous substrate was increased, which was reflected as a decreased Km and an increased Vmax, in accordance with the negative regulatory role of PKC on Kit/SCFR signaling.
Virtual simple architecture (VISA): exceeding the complexity limit in safe real-time systems (2004)

Meeting deadlines is a key requirement in safe real-time systems. Worst-case execution times (WCET) of tasks are needed for safe planning. Contemporary worst-case timing analysis tools can safely and tightly bound execution time on in-order single-issue pipelines with caches and static branch prediction. However, this simple pipeline appears to be a complexity limit, due to the need for analyzability. This excludes a whole class of high-performance processors from many embedded systems. We reconcile the complexity/safety trade-off by decoupling worst-case timing analysis from the processor implementation, through a virtual simple architecture (VISA). A VISA is the timing specification of a hypothetical simple pipeline and is the basis for worst-case timing analysis. However, the underlying microarchitecture can be arbitrarily complex. A task is divided into multiple subtasks which provide a means to gauge progress on the complex pipeline. Each subtask is assigned an interim deadline, or checkpoint, based on the latest allowable completion time of the subtask on the hypothetical simple pipeline. If no checkpoints are missed, then the complex pipeline is as timely as the safe pipeline. If a checkpoint is missed, the pipeline switches to a simple mode of operation that directly implements the VISA so that execution time of unfinished subtasks is safely bounded. The significance of our approach is that we circumvent worst-case timing analysis of the complex pipeline, by dynamically confirming its behavior is bounded by worst-case timing analysis of a simpler proxy pipeline. The benefit of using a high-performance processor is that tasks finish much sooner than they would have on an explicitly-safe processor. The new slack in the schedule can be exploited for higher throughput or lower power. With the VISA approach, an arbitrarily complex SMTprocessor can safely run nonreal-time tasks at the same time as a real-time task. Alternatively, frequency/voltage can be safely lowered to take up slack. We explore the latter application and show a VISA-compliant complex pipeline consumes 43-61% less power than an explicitly-safe pipeline.
Regulation of Inositol 1,4,5-Trisphosphate Receptors (1993)

Inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] is a soluble second messenger responsible for the generation of highly organized Ca2+ signals in a variety of cell types. These Ca2+ signals control many cellular responses, including cell growth, fertilization, smooth muscle contraction and secretion. Ins(1,4,5)P3 is produced at the plasma membrane following receptor activation, but rapidly diffuses into the cytosol, where it binds to specific receptors through which it mobilizes intracellular Ca2+ stores. The actions of Ins(1,4,5)P3 within cells are tightly controlled: enzymes control the rapid generation and metabolism of Ins(1,4,5)P3 following receptor activation; multiple Ins(1,4,5)P3 receptor subtypes and splice variants exist, some of which are differentially expressed between cell types and at different stages of development; and Ins(1,4,5)P3 receptors are the targets for a number of allosteric regulators, including protein kinases, ATP and divalent cations. Understanding how cells control the Ca(2+)-mobilizing activity of Ins(1,4,5)P3 will be important if we are to unravel the mechanisms that underlie the complex arrangements of Ca2+ signals.
Encephalitis associated with Borrelia burgdorferi infection in a horse (1987)

Infection with Borrelia burgdorferi was associated with encephalitis in a horse. The horse lived in an area of Wisconsin endemic for B burgdorferi infection. Borrelia burgdorferi was isolated from the brain, but rabies virus was not detected in the brain. Serum obtained from the horse had a B burgdorferi antibody titer of 1:2,048, but was negative for antibodies to eastern and western encephalomyelitis.
Indoor physical work environment: An ergonomics perspective (2013)

Work in the contemporary era is predominantly performed indoors. Indoor physical environmental factors (temperature, lighting, noise etc.) affect the performance of humans. Generally due consideration is not given to physical environmental aspects while designing individual workstation/workplace/facilities in an organization/industry. Keeping this scenario in mind it was felt that ample information in a consolidated form pertaining to basic indoor environmental parameters will definitely help managers, supervisors, engineers, designers, entrepreneurs to become conscious and take an effort towards implementation of recommendations adopted and proposed by scientific community/organizations for facilities layout planning. Indian standards are moderately addressed to help readers visualize the role of country specific organizations in promoting development of standardization in agreement with international bodies. National institutions help in evolving strategies for formulation and accordingly recognition of standards in their respective countries. Present paper strives to collect, segregate and arrange needed information to represent an appropriate knowledge body for all concerned. Good indoor environment is beneficial in terms of increasing productivity, satisfaction and overall well-being of people. Variations in specifications proposed by different organizations/bodies and other studies were observed. Standards proposed should be used as a basis, while due attention should be given to ethnic and geographic diversity, individual differences and adaptability while designing/ /planning any indoor workplace/workstation/facility. The need of the hour is location/country specific contextual investigations/research into indoor physical environmental factors. Such initiatives will ultimately result in formulation of standards/recommendations which are specific to industry/ location/ intended people.
Automatic Web Services Generation (2009)

This paper discusses the design and implementation of a Service Generator Toolkit (SGT) that allows Web services researchers to easily create large numbers of Web services. When developing a Web services tool, such as a service broker, it is necessary to obtain a large collection of Web services for testing and benchmarking purposes. Since it is difficult to manually create or collect a large number of web services, we chose to implement a system that can automatically generate web services from a service graph model, the SGT. The SGT automatically generates Web services by creating an abstract graph model of the services and then converting that model into implementation files. By converting the services graph model into deployable and executable Web services, the SGT provides support for a greater range of tests than previous efforts that use service models to facilitate testing.
Optimization of Elastically Tailored Tow-Placed Plates with Holes (2003)

Elastic stiffness tailoring of laminated composite panels by allowing the fibers to curve within the plane of the laminate is a design concept that has been demonstrated to be both beneficial and practical. The objective of the present paper is to demonstrate the effectiveness of stiffness tailoring through the use of curvilinear fibers to reduce stress concentrations around the hole and improve the load carrying capability of panels. Preliminary panel designs that are to be manufactured and tested were determined through design studies for flat plates without holes under axial compression using an optimization program. These candidate designs were then analyzed with finite element models that accurately reflect the test conditions and geometries in order to decide upon the final designs for manufacture and testing. An advanced tow-placement machine is used to manufacture the test panels with varying fiber orientation angles. A total of six large panels measuring three feet by six feet, each of which is used to produce four specimens with or without holes, are fabricated. The panels were machined into specimens with holes and tested at NASA Langley Research Center. Buckling response and failure of panels without holes and with two different hole dimensions are presented. Buckling and failure loads of tow-steered specimens are significantly greater than the buckling and failure loads of traditional straight-fiber specimens.
Ideal Gas Solubilities and Solubility Selectivities in a Binary Mixture of Room-Temperature Ionic Liquids (2008)

This study focuses on the solubility behaviors of CO2, CH4, and N2 gases in binary mixtures of imidazolium-based room-temperature ionic liquids (RTILs) using 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C2mim][Tf2N]) and 1-ethyl-3-methylimidazolium tetrafluoroborate ([C2mim][BF4]) at 40 degrees C and low pressures (approximately 1 atm). The mixtures tested were 0, 25, 50, 75, 90, 95, and 100 mol % [C2mim][BF4] in [C2mim][Tf2N]. Results show that regular solution theory (RST) can be used to describe the gas solubility and selectivity behaviors in RTIL mixtures using an average mixture solubility parameter or an average measured mixture molar volume. Interestingly, the solubility selectivity, defined as the ratio of gas mole fractions in the RTIL mixture, of CO2 with N2 or CH4 in pure [C2mim][BF4] can be enhanced by adding 5 mol % [C2mim][Tf2N].
100 Mbit per second VLSI implementation of sliding window coding (2002)

A VLSI circuit is being designed that performs lossless compression and decompression of computer files. The compression ratio must be comparable to that of widely used software methods (2.9 bits/byte) and the speed must be 100 Megabit per second (rate of uncompressed data). Further the design must be sufficiently generic so that it can be adapted to specific requirements.
Gene Expression Patterns in Pancreatic Tumors, Cells and Tissues (2007)

The scope of the present work was enhanced by the inclusion of publicly available datasets that encompass a wide spectrum of human tissues and enabled the identification of candidate genes that may serve diagnostic and therapeutic goals.