gebhart/patrepeval huggingface dataset:
New Enzymatic Method with Tryptophanase for Determining Potassium in Serum (1992)

We established a simple and rapid enzymatic method for measuring potassium ion in serum by using tryptophanase (EC 4.1.99.1) purified from Escherichia coli K12 strain (E. coli K12 IFO 3301). The presence of pyridoxal 5-phosphate promotes this enzymatic reaction, and potassium and (or) ammonium ions further accelerate it, with ammonium and potassium ions providing equivalent acceleration. We eliminated endogenous ammonium ion by using glutamate dehydrogenase (GLDH; EC 1.4.1.4), then produced ammonium ion in the presence of tryptophanase, tryptophan, and pyridoxal 5-phosphate. The concentration of formed ammonium ion, which was proportional to that of potassium ion in sample, was determined by adding GLDH to produce NADP+ in the presence of 2-oxoglutarate and NADPH; we then read the change of absorbance at 340 nm. The standard curve was linear for potassium ion concentrations up to 7.00 mmol/L. The within-assay variation (CV) was 0.89% at 5.51 mmol/L and 1.32% at 3.37 mmol/L. The day-to-day CVs were 0.99% at 6.85 mmol/L and 1.71% at 3.52 mmol/L. Analytical recoveries ranged from 98.7% to 108.9%. The correlation coefficient between values obtained with this enzymatic assay (y) and by flame photometry (x) was 0.995: y = 0.984x + 0.091 mmol/L (Sy.x = 0.105, n = 100). The presence of hemoglobin, bilirubin, or other cations little affects this system.
LPS induces the interaction of a transcription factor, LPS-induced TNF-α factor, and STAT6(B) with effects on multiple cytokines (2005)

TNF-alpha is a pivotal cytokine whose overproduction can be lethal. Previously, we identified a transcription factor, LPS-induced TNF-alpha factor (LITAF), that regulates TNF-alpha transcription. We now report the discovery and characterization of a regulatory cofactor that we call signal transducer and activator of transcription (STAT) 6(B) because of its considerable homology to STAT6 [here referred to as STAT6(A)]. The STAT6(B) gene expression was found to be activated by LPS. Furthermore, we show that cotransfection of STAT6(B) and LITAF induces an interaction between the two proteins, consequently forming a complex that subsequently translocates into the nucleus and up-regulates the transcription of cytokines. The effect of the complex on a panel of cytokines was tested. In addition, the specific role of LITAF in this complex was established with experiments, including RNA interference technology. Overall, these findings describe roles for LITAF, STAT6(B), and the LITAF-STAT6(B) complex in the regulation of inflammatory cytokines in response to LPS stimulation in mammalian cells.
Total synthesis of tetracyclic triterpenes. 1. The (.+-.)-5-epi-euphane ring system (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of tetracyclic triterpenes. 1. The (.+-.)-5-epi-euphane ring systemLawrence Kolaczkowski and William ReuschCite this: J. Org. Chem. 1985, 50, 24, 4766–4771Publication Date (Print):November 1, 1985Publication History Published online1 May 2002Published inissue 1 November 1985https://pubs.acs.org/doi/10.1021/jo00224a022https://doi.org/10.1021/jo00224a022research-articleACS PublicationsRequest reuse permissionsArticle Views625Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Buffer-Free GeSn with High Relaxation Degree Grown on Si(001) Substrate for Photodetection (2018)

In this paper, buffer-free germanium-tin (GeSn) films on Si(001) grown by molecular beam epitaxy are characterized. GeSn layers show high thermal stability under complementary metal-oxide-semiconductor processing conditions. The experimental results demonstrate that Ge <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.928</sub> Sn <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0.072</sub> film retains high crystallinity with few misfit dislocations formed at a relaxation degree of 91.5% after post-thermal annealing at 600 °C. Moreover, numerical simulations demonstrate that the proposed photodetector can achieve a high responsivity of 0.102 A/W at 2 μm. This strategy can facilitate the fabrication of GeSn-based devices, which will contribute substantially to the development of group IV-based infrared optoelectronics.
Observation of soliton self-frequency shift in photonic crystal fibre (2002)

Results from a study in which Raman-shifted outputs were observed from photonic crystal fibres pumped by 800 nm femtosecond pulses are reported. Under optimum conditions, a spectral shift to wavelengths of 1260 nm was recorded.
Reflective point-diffraction microscopic interferometer with long-term stability (2011)

An on-axis phase-shifting reflective point-diffraction microscopic interferometer for quantitative phase microscopy based on Michelson architecture is proposed. A cube beamsplitter splits the object wave spectrum into two copies within two arms. Reference wave is rebuilt in one arm by low-pass filtering on the object wave frequency spectrum with a pinhole-mask mirror, and interferes with the object wave from the other arm. Polarization phase-shifting is performed and phase imaging on microscale specimens is implemented. The experimental results demonstrate that the proposed scheme has the advantage of long-term stability due to its quasi common-path geometry with full use of laser energy.
Free radical photopolymerization initiated by UV and LED: Towards UV stabilized, tack free coatings (2014)

UV stabilizers (UV absorbers and Hindered Amine Light Stabilizers-HALS) are added to UV-curable formulations to provide long-term durability. The influence of HALS and the filtering effect of UV absorbers are investigated under UV and LED irradiation by using different photoinitiating systems in free radical photopolymerization. The contribution of irradiation conditions and initiating systems on the filtering effect is discussed by performing Real-Time Fourier Transform Infrared Spectroscopy (RT-FTIR) and Confocal Raman Microscopy. Additionally, to get quantitative information about the light absorbed by the photoinitiator, without and with the filtering issue arising from UV stabilizers, series of calculations are carried out by taking into account the amount of light absorbed by the compounds and the intensity of the incident light. Finally, formulations are optimized with respect to surface inhibition to obtain tack free coatings in air atmosphere in the presence of UV stabilizers under visible light. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 3597–3607
<i>Caulobacter</i> PopZ forms an intrinsically disordered hub in organizing bacterial cell poles (2016)

Despite their relative simplicity, bacteria have complex anatomy at the subcellular level. At the cell poles of Caulobacter crescentus, a 177-amino acid (aa) protein called PopZ self-assembles into 3D polymeric superstructures. Remarkably, we find that this assemblage interacts directly with at least eight different proteins, which are involved in cell cycle regulation and chromosome segregation. The binding determinants within PopZ include 24 aa at the N terminus, a 32-aa region near the C-terminal homo-oligomeric assembly domain, and portions of an intervening linker region. Together, the N-terminal 133 aa of PopZ are sufficient for interacting with all binding partners, even in the absence of homo-oligomeric assembly. Structural analysis of this region revealed that it is intrinsically disordered, similar to p53 and other hub proteins that organize complex signaling networks in eukaryotic cells. Through live-cell photobleaching, we find rapid binding kinetics between PopZ and its partners, suggesting many pole-localized proteins become concentrated at cell poles through rapid cycles of binding and unbinding within the PopZ scaffold. We conclude that some bacteria, similar to their eukaryotic counterparts, use intrinsically disordered hub proteins for network assembly and subcellular organization.
Influence of external mirror on modal behavior of twin-stripe index-guided injection lasers (1991)

The coupled-mode theory of parallel waveguides is used to calculate the influence of an external mirror on the modal behavior of the AR-coated laser array. The case of twin-stripe index-guided injection lasers with separate contacts is treated in detail. It is shown that for asymmetrical current injection, the external cavity couples the laser's guided array modes and creates new laser-cavity modes with a better mode discrimination in favour of the one having a single far-field pattern. These results imply that multiple laser arrays might benefit from an additional external mirror.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
CLINICAL EVALUATION OF A TWO‐SITE IMMUNORADIOMETRIC ASSAY FOR ADRENOCORTICOTROPIC IN UNEXTRACTED HUMAN PLASMA USING MONOCLONAL ANTIBODIES (1987)

We have developed a sensitive two-site immunoradiometric assay (IRMA) for intact ACTH and its precursors, pro-opiomelanocortin and 22 kDa peptide in unextracted human plasma. The assay uses two monoclonal antibodies. Antibody 1A12, specific for ACTH 10-18, is radiolabelled and antibody 2A3 specific for the C-terminal region (ACTH 24-39), is coupled to Sephacryl S300 for the solid-phase. Samples are incubated for 18 h with labelled antibody followed by 2 h with solid-phase antibody. Separation employs the sucrose layering technique. Using human pituitary ACTH 1-39 (code 74/555) in diluent containing 10% horse serum to standardize the assay, the sensitivity (upper 99% confidence limit of zero standard) is 3.5 +/- 0.8 ng/l (n - 7). The mean coefficient of variation is 5.9% within-assay and 6.7% between-assay and is less than 10% between 22 and greater than 5000 ng/l. Mean recovery of ACTH 1-39 added to dexamethasone-suppressed human plasma is 109% and endogenous ACTH behaves indistinguishably from standard ACTH on dilution. In normal subjects, mean plasma ACTH levels are 30 ng/l at 0730 h, and 15 ng/l at 1630 h at rest. ACTH concentrations are between 60 and 330 ng/l, 8-10.5 h after metyrapone (2 g orally at 2300 h), between 140 and 320 ng/l, 30-60 min after insulin-induced hypoglycaemia, and less than 4 ng/l, 8 h after dexamethasone (1.5 mg orally at 2300 h). In a range of pathological conditions ACTH concentrations accurately reflect the disorders of the pituitary-adrenal axis. Endogenous ACTH immunoactivity is stable in vitro at 22 degrees C for at least 1 h in whole blood and at least 4 h in plasma. It is concluded that this two-site IRMA for ACTH in unextracted plasma offers a reliable assay for clinical purposes.
Anticancer activity of natural and synthetic acetylenic lipids (2006)

This review is a comprehensive survey of acetylenic lipids and their derivatives, obtained from living organisms, that have anticancer activity. Acetylenic metabolites belong to a class of molecules containing triple bond(s). They are found in plants, fungi, microorganisms, and marine invertebrates. Although acetylenes are common as components of terrestrial plants, fungi, and bacteria, it is only within the last 30 years that biologically active polyacetylenes having unusual structural features have been reported from plants, cyanobacteria, algae, invertebrates, and other sources. Naturally occurring aquatic acetylenes are of particular interest since many of them display important biological activities and possess antitumor, antibacterial, antimicrobial, antifouling, antifungal, pesticidal, phototoxic, HIV-inhibitory, and immunosuppressive properties. There is no doubt that they are of great interest, especially for the medicinal and/or pharmaceutical industries. This review presents structures and describes cytotoxic and anticancer activities only for more than 300 acetylenic lipids and their derivatives isolated from living organisms.
Insulin Receptor Carbohydrate Units Contain Poly-<i>N</i>-Acetyllactosamine Chains* (1990)

The insulin receptor was immunoprecipitated from cultured human lymphocytes (IM-9) and rat hepatocytes (Fao) after biosynthetic labeling with [3H]glucosamine or [3H]mannose, and the nature of the carbohydrate units was investigated. Digestion of the receptor from IM-9 lymphocytes with E. freundii endo-beta-galactosidase increased the migration of the insulin receptor alpha- and beta-subunits on sodium dodecyl sulfate-polyacrylamide gels and sharpened the electrophoretic bands; the alpha-subunit was converted from an apparent mol wt (Mr) of 123,000 to a Mr of 118,000, and the beta-subunit from a Mr of 92,000 to 89,000. The susceptibility of the insulin receptor to this enzyme indicates that its carbohydrate units contain poly-N-acetyllactosamine sequences. Affinity chromatography of receptor glycopeptides on Concanavalin-A-Sepharose revealed that the poly-N-acetyllactosamine units were attached to multiantennary glycopeptides that accounted for over 75% of the [3H]glucosamine incorporated into the IM-9 lymphocyte insulin receptor; the remaining radioactivity was present in polymannose units (primarily Man8GlcNAc2) and biantennary complex saccharides. Several differences in the carbohydrate chains of the insulin receptor from the Fao and IM-9 cells indicated that glycosylation was cell specific despite the occurrence of poly-N-acetyllactosamine chains in both cell types. The IM-9 lymphocyte receptor glycopeptides were larger (Mr, 3,200-9,500) and more susceptible to endo-beta-galactosidase than those from the Fao receptor (Mr, 3,000-5,000). Moreover, the released saccharides from the Fao receptor were found by exoglycosidase digestions and chromatographic comparison to standards to contain terminal sialic acid in both alpha 2----3 and alpha 2----6 linkage to galactose, whereas the IM-9 carbohydrate units contained only alpha 2----3-linked sialic acid.
Regions of Lower Crossing Over Harbor More Rare Variants in African Populations of <i>Drosophila melanogaster</i> (2001)

A correlation between diversity levels and rates of recombination is predicted both by models of positive selection, such as hitchhiking associated with the rapid fixation of advantageous mutations, and by models of purifying selection against strongly deleterious mutations (commonly referred to as "background selection"). With parameter values appropriate for Drosophila populations, only the first class of models predicts a marked skew in the frequency spectrum of linked neutral variants, relative to a neutral model. Here, we consider 29 loci scattered throughout the Drosophila melanogaster genome. We show that, in African populations, a summary of the frequency spectrum of polymorphic mutations is positively correlated with the meiotic rate of crossing over. This pattern is demonstrated to be unlikely under a model of background selection. Models of weakly deleterious selection are not expected to produce both the observed correlation and the extent to which nucleotide diversity is reduced in regions of low (but nonzero) recombination. Thus, of existing models, hitchhiking due to the recurrent fixation of advantageous variants is the most plausible explanation for the data.
Nearly cut-off modes caused by diffusion in lithium niobate (1994)

We have observed more extraordinary polarized guided modes near cut-off in titanium in-diffused lithium niobate planar waveguides than are predicted by titanium in-diffusion models. We also observe these nearly cut-off modes in lithium niobate specimens fabricated without titanium under a variety of diffusion atmospheres. We found more guided modes, with higher effective index in these plain lithium niobate specimens fabricated in wet oxygen than in specimens fabricated in either air, dry oxygen, dry argon, or wet argon. Values of the effective index of these nearly cut-off modes found in plain lithium niobate planar waveguides do not agree with those calculated using the lithium out-diffusion model for either vacuum-diffused or oxygen-diffused lithium niobate waveguides. We found good agreement between measured values of the effective mode index in specimens fabricated in either dry oxygen or air and calculated values found using an empirical diffusion model of a step index profile whose depth increases as the square root of diffusion time.
Site-directed mutagenesis of the juxtamembrane domain of the human insulin receptor (1993)

We have studied the functions of the juxtamembrane domain (941-989) of the human insulin receptor by site-directed mutagenesis. Tyrosine phosphorylation of pp185 was impaired in Chinese hamster ovary cells expressing the receptors with the alteration of Tyr960, but not of Tyr953 or Tyr972, to Phe (CHO-Y960F cells) as compared with cells expressing the normal receptors. In CHO-Y960F cells, tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1), the activation of phosphatidylinositol 3-kinase in the anti-phosphotyrosine and anti-IRS-1 immunoprecipitates, the activation of mitogen-activated protein (MAP) kinase, and biological actions were also impaired. In addition, although the deletion of residues 954-965 severely impaired insulin internalization, the deletion of NPXY (957-960), the internalization signal of the low density lipoprotein receptor, did not affect internalization. Moreover, neither the deletions around Tyr953 nor the alterations of the tyrosines (953, 960, or 972) significantly reduced internalization. These data suggest that: 1) Tyr960 is important for the recognition of pp185/IRS-1, the association of phosphatidylinositol 3-kinase with pp185/IRS-1, and the activation of MAP kinase; 2) MAP kinase may lie downstream of pp185/IRS-1 in insulin's signal transduction; and 3) the juxtamembrane domain, but not NPXY or individual tyrosines, is important for insulin internalization.
To Call or To Be Called Behind NATs is Sensitive in Solving the Direct Connection Problem (2010)

In this article, we first depict the call-role sensitivity problem in Network Address Translation (NAT) traversal, and then propose an approach to resolving the problem. The problem is whether a direct connection can be found between two peers across NATs mainly depends on the NAT type at the caller's side. We propose the extra-candidate connectivity check where both peers initiate a direct connectivity check to eliminate the effect of the call role. We have implemented the extra-candidate connectivity check and conducted experiments with 18 different NATs. Experimental results show that our approach can indeed resolve the call-role sensitivity problem, and maximize the direct connectivity rate (DCR) which is improved by 18.71% from the original scheme.
Deep Convolutional Neural Network Features and the Original Image (2016)

Face recognition algorithms based on deep convolutional neural networks (DCNNs) have made progress on the task of recognizing faces in unconstrained viewing conditions. These networks operate with compact feature-based face representations derived from learning a very large number of face images. While the learned features produced by DCNNs can be highly robust to changes in viewpoint, illumination, and appearance, little is known about the nature of the face code that emerges at the top level of such networks. We analyzed the DCNN features produced by two face recognition algorithms. In the first set of experiments we used the top-level features from the DCNNs as input into linear classifiers aimed at predicting metadata about the images. The results show that the DCNN features contain surprisingly accurate information about the yaw and pitch of a face, and about whether the face came from a still image or a video frame. In the second set of experiments, we measured the extent to which individual DCNN features operated in a view-dependent or view-invariant manner. We found that view-dependent coding was a characteristic of the identities rather than the DCNN features - with some identities coded consistently in a view-dependent way and others in a view-independent way. In our third analysis, we visualized the DCNN feature space for over 24,000 images of 500 identities. Images in the center of the space were uniformly of low quality (e.g., extreme views, face occlusion, low resolution). Image quality increased monotonically as a function of distance from the origin. This result suggests that image quality information is available in the DCNN features, such that consistently average feature values reflect coding failures that reliably indicate poor or unusable images. Combined, the results offer insight into the coding mechanisms that support robust representation of faces in DCNNs.
Electrical characterization of redox-active molecular monolayers on SiO2 for memory applications (2003)

Hybrid silicon capacitors have been successfully fabricated by attaching monolayers of redox-active molecules via self-assembly to ultrathin silicon dioxide layers. Capacitance, conductance, and cyclic voltammetric measurements have been used to characterize these capacitors. The presence of distinct capacitance and conductance peaks associated with oxidation and reduction of the monolayers at low gate voltages indicates discrete electron storage states for these capacitors, suggesting their feasibility in memory devices. The inherent molecular scalability and low-power operation coupled with existing silicon technology support the approach of hybrid molecule-silicon devices as a strong candidate for next generation electronic devices.
Rigorous 3D inversion of marine CSEM data based on the integral equation method (2007)

Abstract Marine controlled-source electromagnetic (MCSEM) surveys have become an important part of offshore petroleum exploration. However, due to enormous computational difficulties with full 3D inversion, practical interpretation of MCSEM data is still a very challenging problem. We present a new approach to 3D inversion of MCSEM data based on rigorous integral-equation (IE) forward modeling and a new IE representation of the sensitivity (Fréchet derivative matrix) of observed data to variations in sea-bottom conductivity. We develop a new form of the quasi-analytical approximation for models with variable background conductivity (QAVB) and apply this form for more efficient Fréchet derivative calculations. This approach requires just one forward modeling on every iteration of the regularized gradient-type inversion algorithm, which speeds up the computations significantly. We also use a regularized focusing inversion method, which provides a sharp boundary image of the petroleum reservoir. The methodology is tested on a 3D inversion of the synthetic EM data representing a typical MCSEM survey conducted for offshore petroleum exploration.