gebhart/patrepeval huggingface dataset:
High-throughput single-cell manipulation system for a large number of target cells (2011)

A sequential and high-throughput single-cell manipulation system for a large volume of cells was developed and the successive manipulation for single cell involving single-cell isolation, individual labeling, and individual rupture was realized in a microhydrodynamic flow channel fabricated by using two-dimensional simple flow channels. This microfluidic system consisted of the successive single-cell handlings of single-cell isolation from a large number of cells in cell suspension, labeling each isolated single cell and the lysate extraction from each labeled single cell. This microfluidic system was composed of main channels, cell-trapping pockets, drain channels, and single-cell content collection channels which were fabricated by polydimethylsiloxane. We demonstrated two kinds of prototypes for sequential single-cell manipulations, one was equipped with 16 single-cell isolation pockets in microchannel and the other was constructed of 512 single-cell isolation pockets. In this study, we demonstrated high-throughput and high-volume single-cell isolation with 512 pocket type device. The total number of isolated single cells in each isolation pocket from the cell suspension at a time was 426 for the cell line of African green monkey kidney, COS-1, and 360 for the rat primary brown preadipocytes, BAT. All isolated cells were stained with fluorescence dye injected into the same microchannel successfully. In addition, the extraction and collection of the cell contents was demonstrated using isolated stained COS-1 cells. The cell contents extracted from each captured cell were individually collected within each collection channel by local hydrodynamic flow. The sequential trapping, labeling, and content extraction with 512 pocket type devices realized high-throughput single-cell manipulations for innovative single-cell handling, feasible staining, and accurate cell rupture.
Active skeleton for non-rigid object detection (2009)

We present a shape-based algorithm for detecting and recognizing non-rigid objects from natural images. The existing literature in this domain often cannot model the objects very well. In this paper, we use the skeleton (medial axis) information to capture the main structure of an object, which has the particular advantage in modeling articulation and non-rigid deformation. Given a set of training samples, a tree-union structure is learned on the extracted skeletons to model the variation in configuration. Each branch on the skeleton is associated with a few part-based templates, modeling the object boundary information. We then apply sum-and-max algorithm to perform rapid object detection by matching the skeleton-based active template to the edge map extracted from a test image. The algorithm reports the detection result by a composition of the local maximum responses. Compared with the alternatives on this topic, our algorithm requires less training samples. It is simple, yet efficient and effective. We show encouraging results on two widely used benchmark image sets: the Weizmann horse dataset [7] and the ETHZ dataset [16].
OpenMP-style parallelism in data-centered multicore computing with R (2012)

R 1 is a domain specific language widely used for data analysis by the statistics community as well as by researchers in finance, biology, social sciences, and many other disciplines. As R programs are linked to input data, the exponential growth of available data makes high-performance computing with R imperative. To ease the process of writing parallel programs in R, code transformation from a sequential program to a parallel version would bring much convenience to R users. In this paper, we present our work in semi-automatic parallelization of R codes with user-added OpenMP-style pragmas. While such pragmas are used at the frontend, we take advantage of multiple parallel backends with different R packages. We provide flexibility for importing parallelism with plug-in components, impose built-in MapReduce for data processing, and also maintain code reusability. We illustrate the advantage of the on-the-fly mechanisms which can lead to significant applications in data-centered parallel computing.
The synthesis and structure of the first 10-As-3 species (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe synthesis and structure of the first 10-As-3 speciesScott Anthony Culley and Anthony J. Arduengo IIICite this: J. Am. Chem. Soc. 1985, 107, 4, 1089–1090Publication Date (Print):February 1, 1985Publication History Published online1 May 2002Published inissue 1 February 1985https://pubs.acs.org/doi/10.1021/ja00290a072https://doi.org/10.1021/ja00290a072research-articleACS PublicationsRequest reuse permissionsArticle Views143Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
The demon-haunted sentence: A skeptical analysis of reverse speech (2000)

Advocates of reverse speech propose that it is a direct path to the unconscious mind. However, there is no evidence of its existence, and accepting this pseudoscience could prove tragic.
Feasibility design report : Atiak Moyo Road (2001)

This environmental assessment for the Third Road Development Project for Uganda details expected environmental impacts and proposes measures to mitigate negative impacts. Among them are the following: Minimize hydrological impacts by allowing unimpeded water flow by providing for culverts in the road design. Reduce impacts due to earthworks and quarry excavation by exercising care, and carrying out activities during the dry season. Prevent soil loss and reduce visual intrusion by reinstatement, landscaping of gravel pits and quarries, replanting areas cleared for deviations, and vegetating the road embankment in the steep sections. Introduce speed restrictions to reduce dust emissions. Lessen disturbance caused by noise and dust pollution by locating crushing plants downwind of households. Sensitize motorists and provide special areas for trucks to control oil and noise pollution. Keep deviations within the road reserve as far as practicable. Provide compensation for crops and existing structures on land outside the road reserve temporarily acquired for deviations. The workmen's camp should use gas or electric cookers to preclude the need to but charcoal. A central canteen for the workforce will reduce energy and water consumption and solid waste generated. Conduct campaigns for sexually transmitted diseases (STDs) in the camps as well as in the villages. Provide workers with protective gear and workers compensation cover, and equip sites with first aid kits. Enhance road safety by installing clear and frequent road signs and markings.
Timing analysis for digital fault simulation using assignable delays (1974)

The techniques to be described in this paper are generally applicable to any time domain, parallel fault, digital logic simulation system. The particular implementation was done on the CC-TEGAS3 system and quoted results are from this system.The first technique to be considered provides accuracy of fault simulation when using assignable nominal delays for different element types. The second technique provides for handling fault induced activity in a network, in such a way as to considerably reduce the amount of simulation time required.
Internal sclerostomy using laser ablation of dyed sclera in glaucoma patients: a pilot study. (1993)

Twelve eyes of 12 patients with refractory glaucoma were treated with internal sclerostomy using laser ablation of dyed sclera. The technique involves iontophoretic impregnation of the sclera at the limbal region with 1% methylene blue and subsequent delivery of a pulsed dye laser beam to the stained area through a goniolens. The red wavelength of 664 nm generated by the laser is maximally absorbed by the stained sclera and creates a through and through sclerostomy. Successful complete sclerostomy was achieved in seven eyes (58.3%) but there was some reduction of intraocular pressure (IOP) in all cases. Mean prelaser IOP (of all 12 eyes) was 36.6 mm Hg and 1 hour after therapy it was reduced to 20.0 mm Hg. At the end of the follow-up period (mean 16 months) the mean IOP was 23.7 mm Hg; five eyes (41%) maintained their IOP below 22 mm Hg, and the average number of medications per eye came down from 3.25 to 2.25 per eye. Transient corneal burns with Descemet's membrane folds adjacent to the laser impact zone were detected in eight eyes (67%). No major complications like IOP spike, hyphaema, iridodialysis, or retinal detachment have been detected. Clear cornea and dark blue scleral staining are essential for the mechanical success of this procedure. Gonioscopic laser internal sclerostomy of methylene blue dyed sclera is technically feasible and the preliminary results of IOP control in refractory glaucoma are promising. Modifications of the iontophoresis process and laser parameters are currently under investigation in order to improve these results.
Depth cues in human visual perception and their realization in 3D displays (2010)

Over the last decade, various technologies for visualizing three-dimensional (3D) scenes on displays have been technologically demonstrated and refined, among them such of stereoscopic, multi-view, integral imaging, volumetric, or holographic type. Most of the current approaches utilize the conventional stereoscopic principle. But they all lack of their inherent conflict between vergence and accommodation since scene depth cannot be physically realized but only feigned by displaying two views of different perspective on a flat screen and delivering them to the corresponding left and right eye. This mismatch requires the viewer to override the physiologically coupled oculomotor processes of vergence and eye focus that may cause visual discomfort and fatigue. This paper discusses the depth cues in the human visual perception for both image quality and visual comfort of direct-view 3D displays. We concentrate our analysis especially on near-range depth cues, compare visual performance and depth-range capabilities of stereoscopic and holographic displays, and evaluate potential depth limitations of 3D displays from a physiological point of view.
Susceptibility of Influenza Viruses to the Novel Cap-Dependent Endonuclease Inhibitor Baloxavir Marboxil (2018)

The novel cap-dependent endonuclease inhibitor baloxavir marboxil was approved for the treatment of influenza A and B virus infections in February 2018 in Japan. Because of the need to monitor influenza viruses for reduced susceptibility to this drug, we used two cell-based screening systems - a conventional plaque reduction assay and a focus reduction assay - to evaluate the susceptibility of influenza viruses to baloxavir. First, we generated a reference virus possessing an I38T substitution in the polymerase acidic subunit (PA), which is known to be associated with reduced susceptibility to baloxavir, and demonstrated the validity of our systems using this reference virus. We then determined the susceptibility of a panel of neuraminidase (NA) inhibitor-resistant viruses and their sensitive counterparts to baloxavir. No significant differences in baloxavir susceptibilities were found between the NA inhibitor-resistant and -sensitive viruses. We also examined seasonal influenza viruses isolated during the 2017-2018 influenza season in Japan and found that no currently circulating A(H1N1)pdm09, A(H3N2), or B viruses had significantly reduced susceptibility to baloxavir and none of the viruses possessed an amino acid substitution at PA residue 38. Use of a combination of methods to analyze antiviral susceptibility and detect amino acid substitutions is valuable for monitoring the emergence of baloxavir-resistant viruses.
Prospective Evaluation of Community‐Acquired Acute‐Phase Hepatitis C Virus Infection (2005)

These data underscore the importance of nucleic acid screening of blood donations to prevent HCV transmission and of long-term follow-up to ascertain whether there is viral persistence, at least among injection drug users.
Comparison of bile salt/phosphatidylcholine mixed micelles in solubilization to sterols and stability (2016)

Androst-3β,5α,6β-triol (Triol) is a promising neuroprotective agent, but its poor solubility restricts its development into parenteral preparations. In this study, Triol is significantly solubilized by bile salt/phosphatidylcholine mixed micelles (BS/PC-MM). All BS/PC-MM systems are tested to remarkably improve the drug solubility with various stabilities after drug loading. Among them, the sodium glycocholate (SGC)/egg phosphatidylcholine (EPC) system with 2:1 ratio in weight and the total concentration of SGC and EPC of 100 mg/mL is proved to produce stable mixed micelles with high drug loading. It is found that the stability of drug-loaded mixed micelles is quite different, which might be related to the change in critical micelle concentration (CMC) after incorporating drugs. SGC/EPC and SGC/soya phosphatidylcholine (SPC) remain transparent under accelerated conditions and manifest a decreased CMC (dropping from 0.105 to 0.056 mg/mL and from 0.067 to 0.024 mg/mL, respectively). In contrast, swine bile acid-sodium salt (SBA-Na)/PC and sodium deoxycholate (SDC)/PC are accompanied by drug precipitation and reached the maximum CMC on the first and the third days, respectively. Interestingly, the variation of CMC under accelerated testing conditions highly matches the drug-precipitating event in the primary stability experiment. In brief, the bile salt/phosphatidylcholine system exists as a potential strategy of improving sterol drug solubility. CMC variation under accelerated testing conditions might be a simple and easy method to predict the stability of drug-loaded mixed micelles.
HLA‐B16 antigens: sequence of the ST‐16 antigen, further definition of two B38 subtypes and evidence for convergent evolution of B*3902 (1995)

The ST-16 antigenic specificity of the HLA-B locus is defined as a B39 variant of Mexican-Americans. Nucleotide sequencing of cDNA shows the ST-16 allele (B*3905) differs from B*39011 by a single substitution that substitutes tyrosine for aspartic acid at position 74 of the mature class I heavy chain. The complete coding region sequence for the common caucasoid allele encoding the B38 antigen has been determined. This B*3801 allele differs from B*3802 at two nucleotide substitutions within the Bw4 sequence motif. B*3801 and B*3802 may have been derived independently from B*39011 by conversion events with B alleles donating distinctive Bw4 motifs. A novel allele B*39022 derived from a Colombian Indian differs from the B*39021 allele of Japanese origin at two widely separated silent substitutions. Comparison of sequences for the known B16 alleles suggest that B*39021 and B*39022 were independently derived by recombination from B*39013 and B*39011 respectively.
Mobilization of hematopoietic stem cells: state of the art (2008)

HSC mobilization induced by granulocyte colony-stimulating factor may be enhanced by directly targeting the chemotactic interaction between HSCs and bone marrow stroma with CXCR4 antagonists. When the primary problem is reduced, however, HSC numbers in the bone marrow, due to repeated chemotherapy/radiotherapy treatments, an alternative is to enhance HSC content by enhancing bone formation prior to mobilization.
Activity-sensitive flip-flop and latch selection for reduced energy (2002)

This article presents new techniques to evaluate the energy and delay of flip-flop and latch designs and shows that no single existing design performs well across the wide range of operating regimes present in complex systems. We prepose the use of a selection of flip-flop latch designs, each timed for different activation patterns and speed requirements. We illustrate the use of our technique on a pipelined MIPS processor datapath running SPECint95 benchmarks, where we reduce total flip-flop and latch energy by 60% without increasing cycle time.
Glycoprotein Ia gene C807T polymorphism and risk for major adverse cardiac events within the first 30 days after coronary artery stenting (2000)

The glycoprotein complex Ia/IIa (GP Ia/IIa) is a major collagen receptor on platelets and other cell types. Recently, linked polymorphisms within the coding region of the GP Ia gene (C807T and G873A) were identified that are related to GP Ia/IIa surface expression. The T807/A873 allele is associated with high expression, whereas the C807/G873 allele is associated with low surface expression of GP Ia/IIa. Subsequently, the T807 allele was found to be associated with coronary and cerebral infarction in younger patients. Platelet adhesion to the vessel wall plays a pivotal role in thrombosis after coronary artery stent placement. The goal of this study was to test whether C807T polymorphism is associated with a higher incidence of thrombotic events following coronary stenting. Consecutive patients treated with coronary stent placement (n = 1797) were genotyped for C807T polymorphism with polymerase chain reaction and allele-specific fluorogenic probes. The composite end point was defined as death, myocardial infarction, or urgent target vessel revascularization within 30 days of stent implantation. The genotype distribution of the study population was CC in 36.5%, CT in 46.7%, and TT in 16.8% of the patients. The incidence of the composite end point was 6.5% in T allele carriers and 5.3% in noncarriers (odds ratio for T allele carriage 1.23 [95% confidence interval, 0.81-1.86],P = .33). After adjusting for other baseline characteristics, the odds ratio for the composite end point was 1.15 (0.76-1.75). Therefore, C807T genotype has no significant influence on the major adverse events occurring after coronary artery stenting.
Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11 (1996)

Lactobacillus plantarum C11 secretes a small cationic peptide, plantaricin A, that serves as induction signal for bacteriocin production as well as transcription of plnABCD. The plnABCD operon encodes the plantaricin A precursor (PlnA) itself and determinants (PlnBCD) for a signal transducing pathway. By Northern (RNA) and sequencing analyses, four new plantaricin A-induced operons were identified. All were highly activated in concert with plnABCD upon bacteriocin induction. Two of these operons (termed plnEFI and plnJKLR) each encompass a gene pair (plnEF and plnJK, respectively) encoding two small cationic bacteriocin-like peptides with double-glycine-type leaders. The open reading frames (ORFs) encoding the bacteriocin-like peptides are followed by ORFs (plnI and -L, respectively) encoding cationic hydrophobic proteins resembling bacteriocin immunity proteins. On the third operon (termed plnMNOP), a similar bacteriocin-like ORF (plnN) and a putative immunity ORF (either plnM or -P) were identified as well. These findings suggest that two bacteriocins of two-peptide type (mature PlnEF and PlnJK) and a bacteriocin of one-peptide type (mature PlnN) could be responsible for the observed bacteriocin activity. The last operon (termed plnGHSTUV) contains two ORFs (plnGH) apparently encoding an ABC transporter and its accessory protein, respectively, known to be involved in processing and export of peptides with precursor double-glycine-type leaders. Promoter structure was established. A conserved regulatory-like box encompassing two direct repeats was identified in the promoter regions of all five plantaricin A-induced operons. These repeats may serve as regulatory elements for gene expression.
Optical constants of electron-beam evaporated boron films in the 68-900 eV photon energy range (2007)

The optical constants of electron-beam evaporated boron from 6.8 to 900 eV were calculated through transmittance measurements of boron thin films deposited onto carbon-coated microgrids or LiF substrates in ultrahigh-vacuum conditions. In the low-energy part of the spectrum the measurements were performed in situ on freshly deposited samples, whereas in the high-energy range the samples were exposed to the atmosphere before the measurements. The extinction coefficient was calculated directly from the transmittance data, and a Kramers-Kronig analysis that combined the current data with data from the literature was performed to determine the dispersive part of the index of refraction. Finally, two different sum-rule tests were performed that indicated the good consistency of the data.
Global Leadership for Sustainability (2021)

Given the social and environmental challenges facing all organizations, there is a need for new leadership models, methods, and tools for implementing organizational change for sustainable development. Toward that end, we review current approaches to leadership for sustainability in terms of their conceptual frameworks and extant research, which all advocate a balanced stakeholder approach to leadership to address the social and environmental issues related to sustainability and sustainable development. Then, drawing from spiritual and being-centered leadership theories, we offer a model of Global Leadership for Sustainability (GLfS) that incorporates and extends the conceptual domain beyond current approaches to leadership for sustainability. In doing so we propose that spirituality, through the qualities of self-transcendence and interconnectedness, is critical for sustainability and is foundational for GLfS. We also emphasize the importance of cultivating a Global Mindset for Sustainability, which incorporates two ethical principles—an ethic of remote moral responsibility and an ethic of care and compassion. As a result, global leaders for sustainability become more committed to moving beyond satisfying stakeholders’ demands for economic returns, toward a more sustainable, triple bottom line, balanced approach. Finally, we discuss implications for theory, research, and practice of GLfS.