gebhart/patrepeval huggingface dataset:
Studies on the Energy Metabolism of Human Leukocytes (1967)

Abstract 1. Oxidative phosphorylation has been studied in mitochondrial preparations from human leukocytes, using recently developed methods for homogenization, measuring respiration, and assaying for ATP. 2. Appreciable stimulation of both respiration and phosphorylation was limited to 3 substrates: succinate, malate, and α-glycerophosphate. The effects of other substrates were minimal. 3. The stimulating effects of these 3 substrates responded to inhibitors in a manner typical of mitochondrial oxidative phosphorylation. There was also considerable endogenous activity which, however, was insensitive to inhibitors. It is concluded the endogenous respiration and phosphorylation are not associated with electron transport. Subtracting their values from the data, P/O ratios consistent with good phosphorylation with the 3 substrates are obtained. 4. Studies with oligomycin and dinitrophenol suggest the presence of respiratory control. This indicates the mitochondria are intact. It is concluded that in the intact leukocyte the mitochondria are a major source of ATP.
Behavioral Phenotyping of Transgenic and Knockout Mice: Practical Concerns and Potential Pitfalls (2006)

New technologies in molecular genetics have dramatically increased the number of targeted gene mutations available to the biomedical research community. Many mutant mouse lines have been generated to provide animal models for human genetic disorders, offering insights into anatomical, neurochemical, and behavioral effects of aberrant gene expression. A variety of assays have been developed to identify and characterize phenotypic changes. In the behavioral domain, our phenotyping strategy involves a comprehensive standardized methodological approach that assesses general health, reflexes, sensory abilities, and motor functions. This assessment is followed by a series of complementary tasks in the specific behavioral domain(s) hypothesized to reveal the function(s) of the gene. Our multitiered approach minimizes intersubject variability by standardizing the experimental history for all animals, improves interlaboratory reliability by providing a clearly defined experimental protocol, and minimizes artifactual interpretations of behavioral data by careful preliminary assessments of basic behaviors, followed by multiple tests within the behavioral domain of interest. Despite meticulous attention to experimental protocol, attention to environmental factors is essential. Differences in noise, light, home cage environment, handling, and diet can dramatically alter behavior. Baseline differences in the behaviors of inbred strains used to generate targeted mutant mouse lines can directly influence the behavioral phenotype of the mutant line. Strategies aimed at minimizing environmental variability and contributions of background genes will enhance the robustness of mouse behavioral phenotyping assays.
First demonstration of air-silica Bragg fiber (2004)

We present experimental and theoretical results on air-silica Bragg fibers. The TE/sub 01/ mode is observed for the first time to our knowledge in Bragg fibers. We could transmit light by bandgap guiding over 50 m.
Face Tracking and Recognition at a Distance: A Coaxial and Concentric PTZ Camera System (2013)

Face recognition has been regarded as an effective method for subject identification at a distance because of its covert and remote sensing capability. However, face images have a low resolution when they are captured at a distance (say, larger than 5 meters) thereby degrading the face matching performance. To address this problem, we propose an imaging system consisting of static and pan-tilt-zoom (PTZ) cameras to acquire high resolution face images up to a distance of 12 meters. We propose a novel coaxial-concentric camera configuration between the static and PTZ cameras to achieve the distance invariance property using a simple calibration scheme. We also use a linear prediction model and camera motion control to mitigate delays in image processing and mechanical camera motion. Our imaging system was used to track 50 different subjects and their faces at distances ranging from 6 to 12 meters. The matching scenario consisted of these 50 subjects as probe and additional 10 000 subjects as gallery. Rank-1 identification accuracy of 91.5% was achieved compared to 0% rank-1 accuracy of the conventional camera system using a state-of-the-art matcher. The proposed camera system can operate at a larger distance (up to 50 meters) by replacing the static camera with a PTZ camera to detect a subject at a larger distance and control the second PTZ camera to capture the high-resolution face image.
Discontinuous electrophoresis revisited: a review of the process. (1996)

The use and development of discontinuous buffer systems for the separation of proteins and nucleic acids are reviewed, and the advantages over a continuous buffer system are presented. Emphasis is given to the more recent applications in DNA separations where discontinuous systems have aided in the resolution and mobility tailoring of DNA fragments. Various DNA size ranges can be separated in short time periods by simple choice of buffer components rather than gel alterations. Guidelines for choosing appropriate buffer systems are offered as well as the application of borate complexes to modify the mobility of DNA. This article reviews the historical development of discontinuous buffers in the electrophoresis of proteins and nucleic acids with emphasis on the characteristics and applicability to nucleic acid separations.
A LDA-based method for automatic tagging of Youtube videos (2013)

This article presents a method for automatic tagging of Youtube videos. The proposed method combines an automatic speech recognition (ASR) system, that extracts the spoken contents, and a keyword extraction component that aims at finding a small set of tags representing a video. In order to improve the robustness of the tagging system to the recognition errors, a video transcription is represented in a topic space obtained by a Latent Dirichlet Allocation (LDA), in which each dimension is automatically characterized by a list of weighted terms. Tags are extracted by combining the weighted word list of the best LDA classes. We evaluate this method by employing the user-provided tags of Youtube videos as reference and we investigate the impact of the topic model granularity. The obtained results demonstrate the interest of such model to improve the robustness of the tagging system.
Automatic Number Plate Recognition (2024)

This final project develops an algorithm for automatic number plate recognition (ANPR). ANPR has gained much interest during the last decade along with the improvement of digital cameras and the gain in computational capacity. \n\t\t\t\t The text is divided in four chapters. The first, introduces the origins of digital \n\t\t\t\t image processing, also a little resume about the following algorithms that are \n\t\t\t\t needed for develop the system ANPR. The second chapter presents the objectives \n\t\t\t\t to be achieved in this project, as well as, the program used for his development. \n\t\t\t\t The following chapter explains the different algorithms that compound the system, \n\t\t\t\t which is built in five sections; the first is the initial detection of a possible number \n\t\t\t\t plate using edge and intensity detection to extract information from the image. The \n\t\t\t\t second and the third step, thresholding and normalization, are necessary to use the \n\t\t\t\t images in the following stages; the text of the plate is found and normalized. With \n\t\t\t\t the segmentation, each character of the plate is isolated for subsequent recognition. The last step reads the characters by correlation template matching, which is a simple but robust way of recognizing structured text with a small set of characters. It is evaluated the system’s speed and his error rate. Finally, the conclusions and future works are shown in the chapter four. The databases used consist of images under normal conditions and only Bulgarian’s numbers plate.
Treatment of bipolar mixed state with olanzapine. (1999)

Olanzapine was well-tolerated and was effective in the acute treatment of bipolar mixed state.
The Importance of Being Normal: Hey Hey It's Esther Blueburger (2008)

The manner in which Cathy Randall's 2008 quirky coming-of-age teen film 'Hey Hey It's Esther Blueburger' deals with a teenager's compulsion to be 'normal' is analysed. It fancifully addresses her experiences of growing up amidst changing relations with family and friends.
Influence of rate of heating on thermosensitivity of L1210 leukemia: membrane lipids and Mr 70,000 heat shock protein. (1986)

We examined the effect of rate of temperature rise on the thermosensitivity of a murine lymphoblastic leukemia. L1210 cells suspended in RPMI 1630 medium:5% fetal bovine serum at pH 7.4 were heated from 37 degrees C-42 degrees C, or 44 degrees C over variable times (immediately, 30, 60, 120, 180 min) in a circulating water bath controlled by an electronic temperature programmer. Survival of the cells using a soft agar clonogenic assay was plotted against the time at final temperature so that a Do (min of heat required to reduce survival by 63% on the exponential portion of the survival curve) could be calculated as an estimate of thermosensitivity. Cells heated from 37 degrees C-42 degrees C over a time period of 30 min (10 degrees C/h) were less thermosensitive (Do 62.7 +/- 12.5 min) as compared to those exposed immediately to 42 degrees C (Do 38.5 +/- 2.2 min). Cells heated over a period of 180 min (1.6 degrees C/h) showed almost no death even after 4 h at 42 degrees C. Thermosensitivity of cells heated to several other high temperatures was also a function of rate of heating. This relative thermal resistance induced by slow heating was not a result of a change in membrane cholesterol content or fatty acid composition. Similarly, there was no difference between cells heated at slow and fast rates in cell cycle distribution or in cellular protein concentration. The major heat shock protein of Mr 70,000, which was induced by immediate heating, was not synthesized at the same high rate 1-12 h after heat treatment by the cells made thermotolerant with slow heating. We conclude that the thermosensitivity of this neoplastic cell can be altered considerably by the rate of heating. This alteration is not due to a change in membrane lipids. Furthermore, the heat shock protein at Mr 70,000 which was synthesized after immediate heating could not be demonstrated in the gradually heated L1210 leukemia cells.
A New type of permanent magnet synchronous motors for parallel hybrid electric vehicle (2009)

Halbach motor is a novel permanent magnet (PM) motor. One-sided and sinusoidal field is produced by the special distribution of permanent magnets. The field can increase air-gap flux and decrease the rotor yoke flux, which can improve power density, efficiency and reduce volume and pulsating torque of motor. It accords with hybrid electric vehicle system's need: high efficiency, high power density, low pulsating torque. Full-digital drive system of hybrid electric vehicle based on DSP2407A is designed according to Halbach motor's distinguishing feature. Experiment results prove Halbach motor has much more advantages in the system than traditional permanent magnetic motor.
Aspartate Isomerization in the Complementarity-Determining Regions of Two Closely Related Monoclonal Antibodies (2007)

The aspartic acid residues (Asp) present in the complementarity-determining regions (CDRs) of the light chains of two recombinant monoclonal antibodies (MAbs), MAb I and MAb II, are highly susceptible to isomerization due to the presence of glycine residues (Gly) on their C-terminal ends. Asp isomerization in these MAbs leads to formation of the isoaspartate (IsoAsp) and the cyclic imide (Asu) variants of these MAbs. Both MAb I and MAb II, employed in this study, elicit their pharmacological responses through binding human IgE. The formation of the MAb variants as a result of Asp isomerization significantly reduces the binding affinities of these antibodies to IgE, thereby reducing their potencies. Here we report on significant differences in the susceptibility of the MAb I and the MAb II to Asp isomerization. The molecular basis for these differences in rates of Asp isomerization was elucidated. The effect of primary sequence on Asp isomerization was evaluated using pentapeptide models of the MAbs, which included the labile Asp residues and their neighboring amino acid residues. The separation of the parent MAbs and pentapeptides from their isomerization products was achieved using hydrophobic interaction chromatography (HIC) and rp-HPLC, respectively. Structural characterization of the MAbs was performed using differential scanning calorimetry (DSC), circular dichroism (CD), and X-ray crystallography. Our investigations demonstrate that the differences in the Asp isomerization rates between MAb I and MAb II can be attributed to structural factors including the conformational flexibility and the extent of solvent exposure of the labile Asp residue.
Human Monocyte Recognition of Adenosine-Based Cyclic Dinucleotides Unveils the A2a G<sub>αs</sub>Protein-Coupled Receptor Tonic Inhibition of Mitochondrially Induced Cell Death (2014)

Cyclic dinucleotides are important messengers for bacteria and protozoa and are well-characterized immunity alarmins for infected mammalian cells through intracellular binding to STING receptors. We sought to investigate their unknown extracellular effects by adding cyclic dinucleotides to the culture medium of freshly isolated human blood cells in vitro. Here we report that adenosine-containing cyclic dinucleotides induce the selective apoptosis of monocytes through a novel apoptotic pathway. We demonstrate that these compounds are inverse agonist ligands of A2a, a Gαs-coupled adenosine receptor selectively expressed by monocytes. Inhibition of monocyte A2a by these ligands induces apoptosis through a mechanism independent of that of the STING receptors. The blockade of basal (adenosine-free) signaling from A2a inhibits protein kinase A (PKA) activity, thereby recruiting cytosolic p53, which opens the mitochondrial permeability transition pore and impairs mitochondrial respiration, resulting in apoptosis. A2a antagonists and inverse agonist ligands induce apoptosis of human monocytes, while A2a agonists are antiapoptotic. In vivo, we used a mock developing human hematopoietic system through NSG mice transplanted with human CD34(+) cells. Treatment with cyclic di-AMP selectively depleted A2a-expressing monocytes and their precursors via apoptosis. Thus, monocyte recognition of cyclic dinucleotides unravels a novel proapoptotic pathway: the A2a Gαs protein-coupled receptor (GPCR)-driven tonic inhibitory signaling of mitochondrion-induced cell death.
Rock Springs Site 12 hydraulic/explosive true in situ oil shale fracturing experiment (1980)

The experiment plan involved the creation and characterization of three horizontal hydraulic fractures, followed by the insertion and simultaneous detonation of slurry explosive in the two lower fractures. Core analyses, wellbore logging, and airflow and /sup 85/Kr tracer tests were used for site characterization and assessment of the hydraulic and explosive fracturing. Tiltmeters, wellhead pressure and flow gages, and in-formation pressure, flow and crack-opening sensors were used to monitor hydrofracture creation and explosive insertion. Explosive detonation diagnostic data were taken with stress and time-of-arrival gages and surface and in-formation accelerometers. The post-fracturing assessments indicated that: (1) hydrofracture creation and explosive insertion and detonation were accomplished essentially as planned; (2) induced fractures were randomly distributed through the shale with no extensively fractured regions or dislocation of shale; and (3) enhancement of permeability was limited to enlargement of the explosive-filled fractures.
Epidermal Growth Factor Receptors in Cancer Tissues of Esophagus, Lung, Pancreas, Colorectum, Breast and Stomach (1988)

The levels of epidermal growth factor (EGF) receptors were investigated in surgically resected tumors of various origins including esophagus (n = 33), lung (n = 14), pancreas (n = 9), colorectum (n = 10), breast (n = 23) and stomach (n = 8). The 125I-EGF binding capacities of squamous cell carcinomas of esophagus and lung were exceptionally higher than those of the other cancer tissues. Immunohistochemical staining with an anti-EGF receptor monoclonal antibody detected EGF receptors in the basal cells and parabasal cells of normal esophageal epithelium and in all the cancer cells of squamous cell carcinoma tissues of esophagus and lung. DNA replicating cells were examined by the bromodeoxyuridine staining method and it was found that the basal cells and parabasal cells of normal epithelium and peripheral cells of cancer pearls are proliferating. Contrary to this, a tumor antigen TA-4, known as a specific marker for squamous carcinoma, was detected in the differentiated cancer cells and in middle-layer squamous cells. These results strongly suggest that the increase in EGF receptor levels may be associated with the development of human squamous cell cancers of esophagus and lung. Thus, measurement of EGF receptor expression in tumor tissues has diagnostic value and should prove useful for the development of new therapies.
Clinical and pathological analysis of transplant glomerulopathy cases (2012)

Abstract Transplant glomerulopathy ( TG ) is involved in the criteria of chronic active antibody‐mediated rejection (c‐ AMR ) in B anff ‘09 classification. Patients TG was diagnosed in 58 renal allograft biopsy specimens ( BS ) from 37 renal transplant patients. Results Among 58 BS of TG , 27 BS were mild (cg1), 16 were moderate (cg2), and 15 were severe (cg3). Peritubular capillaritis was present in 49 (84%), transplant glomerulitis was seen in 47 BS (81%), interstitial fibrosis and tubular atrophy in 47 (81%) and the thickening of the peritubular capillary ( PTC ) basement membrane ( PTCBM ) in 44 (76%), and interstitial inflammation was present in 26 BS (45%). C 4d deposition in PTC was presented in 32 BS (55%). The circulating anti‐ HLA alloantibody was detected in 38 times (76%), of which 27/38 (54%) were donor‐specific antibodies. Deterioration of renal allografts’ function after biopsies was seen in 12 patients (32%) with six of them lost their graft. Conclusions We suggest that histopathological changes of TG accompanying by transplant glomerulitis, peritubular capillaritis, the thickening of the PTCBM , and circulating anti‐ HLA antibodies might indicate c‐ AMR , even if C 4d deposition in PTC is negative. The prognosis of the graft exhibiting TG was relatively good under the present immunosuppression protocol in short time.
Mesocrystals—Ordered Nanoparticle Superstructures (2009)

Mesocrystals are 3D ordered nanoparticle superstructures, often with internal porosity, which receive much recent research interest. While more and more mesocrystal systems are found in biomineralization or synthesized, their potential as material still needs to be explored. It needs to be revealed, which new chemical and physical properties arise from the mesocrystal structure, or how they change by the ordered aggregation of nanoparticles to fully exploit the promising potential of mesocrystals. Also, the mechanisms for mesocrystal synthesis need to be explored to adapt it to a wide class of materials. The last three years have seen remarkable progress, which is summarized here. Also potential future directions of this reaserch field are discussed. This shows the importance of mesocrystals not only for the field of materials research and allows the appliction of mesocrystals in advanced materials synthesis or property improvement of existing materials. It also outlines attractive research directions in this field.
Theory of Prose (1991)

Journal Article Theory of Prose Get access Theory of Prose. BY Victor Shklovsky, TRANSLATED BY Benjamin Sher. Dalkey Archive Press. £29.95. David Seed David Seed Search for other works by this author on: Oxford Academic Google Scholar English: Journal of the English Association, Volume 40, Issue 166, Spring 1991, Pages 86–92, https://doi.org/10.1093/english/40.166.86 Published: 01 March 1991
Chemical and physical analyses of wax ester properties (2001)

Wax esters are major constituents of the surface lipids in many terrestrial arthropods, but their study is complicated by their diversity. We developed a procedure for quantifying isomers in mixtures of straight-chain saturated and unsaturated wax esters having the same molecular weights, using single-ion monitoring of the total ion current data from gas chromatography-mass spectrometry. We examined the biological consequences of structural differences by measuring the melting temperatures, T(m), of >60 synthetic wax esters, containing 26-48 carbon atoms. Compounds containing saturated alcohol and acid moieties melted at 38-73 degrees C. The main factor affecting T(m) was the total chain length of the wax ester, but the placement of the ester bond also affected T(m). Insertion of a double bond into either the alcohol or acid moiety decreased T(m) by approximately 30 degrees C. Simple mixtures of wax esters with n-alkanes melted several degrees C lower than predicted from the melting points of the component lipids. Our results indicate that the wax esters of primary alcohols that are most typically found on the cuticle of terrestrial arthropods occur in a solid state under physiological conditions, thereby conferring greater waterproofing. Wax esters of secondary alcohols, which occur on melanopline grasshoppers, melted >60 degrees C below primary esters of the same molecular weight and reduced T(m) of the total surface lipids to environmental values.
Comprehensive study of the Raman shifts of strained silicon and germanium (2009)

Raman shifts are investigated on silicon and germanium substrates under the uniaxial tensile strain on various substrate orientations. The strain splits the triply degenerate optical (LO, TO) phonons at the zone center (k⃗≈0). The redshifts of Si Raman peaks induced by the tensile strain on all substrate orientations are observed. With the specific polarization of the incident light, however, the unusual blueshifts of Ge Raman peaks induced by the tensile strain are observed on (110) and (111) Ge substrates. By using the suitable phenomenological constants and taking the Raman selection rules into consideration, the experimental results are in reasonable agreement with the lattice dynamical theory.
Circulating Leukemia Inhibitory Factor Levels Correlate With Disease Severity In Meningococcemia (1994)

Circulating concentrations of the proinflammatory cytokine leukemia inhibitory factor (LIF) were prospectively determined by radioreceptor competition assay (sensitivity, 1 ng/mL) in 33 subjects with meningococcemia. LIF was detected in the plasma of 13 subjects and was associated with development of septic shock (P < .01), disseminated intravascular coagulation (P < .05), multiorgan failure (P < .05), and death (P < .01). Plasma LIF concentrations were highest (1-1772 ng/mL) at hospital admission and became undetectable within 36 h, and the peak levels correlated inversely with systolic blood pressure (r, -.70, P < .001), peripheral blood leukocyte count (r, -.58, P < .01), and prodromal interval (r, -.60, P < .001). Plasma LIF concentrations > 400 ng/mL were present only in subjects with fatal fulminant infection. LIF concentrations in plasma collected within 12 h of hospital admission correlated with disease severity in patients with meningococcemia. It is likely that LIF participates in the host response to infection, and it may contribute to the pathogenesis of septic shock.