gebhart/patrepeval huggingface dataset:
Influence of furosemide on hemodynamic responses during exercise in horses (1992)

Four hours prior to exercise on a high-speed treadmill, 4 dosages of furosemide (0.25, 0.50, 1.0, and 2.0 mg/kg of body weight) and a control treatment (10 ml of 0.9% NaCl) were administered IV to 6 horses. Carotid arterial pressure (CAP), pulmonary arterial pressure (PAP), and heart rate were not different in resting horses before and 4 hours after furosemide administration. Furosemide at dosage of 2 mg/kg reduced resting right atrial pressure (RAP) 4 hours after furosemide injection. During exercise, increases in treadmill speed were associated with increases in RAP, CAP, PAP, and heart rate. Furosemide (0.25 to 2 mg/kg), administered 4 hours before exercise, reduced RAP and PAP during exercise in dose-dependent manner, but did not influence heart rate. Mean CAP was reduced by the 2-mg/kg furosemide dosage during exercise at 9 and 11 m/s, but not at 13 m/s. During recovery, only RAP was decreased by furosemide administration. Plasma lactate concentration was not significantly influenced by furosemide administration. Furosemide did not influence PCV or hemoglobin concentration at rest prior to exercise, but did increase both variables in dose-dependent manner during exercise and recovery. However, the magnitude of the changes in PCV and hemoglobin concentration were small in comparison with changes in RAP and PAP, and indicate that furosemide has other properties in addition to its diuretic activities. Furosemide may mediate some of its cardiopulmonary effects by vasodilatory activities that directly lower pulmonary arterial pressure, but also increase venous capacitance, thereby reducing venous return to the atria and cardiac filling.
Study on Thermodynamic Properties of Ionic Liquid <i>N</i>-Butyl-3-methylpyridinium Bis(trifluoromethylsulfonyl)imide (2012)

An air- and water-stable hydrophobic ionic liquid N-butyl-3-methylpyridinium bis(trifluoromethylsulfonyl)imide ([c43mpy][NTf2]) was synthesized and characterized. Density, surface tension, dynamic viscosity, and electrical conductivity of the IL were measured and calculated from (278.15 to 353.15) K. The glass transition temperature and decomposition temperature were determined by the differential scanning calorimetry and thermogravimatric analysis. The physicochemical properties like molecular volume, standard molar entropy, lattice energy, parachor, molar enthalpy of vaporization, interstice volume, thermal expansion coefficient, interstice fraction, etc. of the IL were estimated by the reported empirical and semiempirical equations. The dynamic viscosity and electrical conductivity data of the IL were described by Vogel–Fulcher–Tamman and Arrhenius equations, respectively. Then, the relationship between the molar conductivity and dynamic viscosity of this IL was expressed through the Walden rule.
Effectively Generating and Delivering Personalized Product Information: Adopting the Web 2.0 Approach (2010)

E-catalogues are widely used for presenting product information in e-commerce environment. Currently, many companies send product information to potential customers via e-mails, but there is a spam problem preventing such e-mails from reaching their target customers. Nevertheless, personalization is now considered as an important marketing strategy, so that mass distribution of e-catalogues may become less desirable for companies. In this study, we developed an innovative service platform for efficiently creating and effectively delivering personalized e-catalogues to target customers. This study not only utilizes but contributes to the body of knowledge associated with the emergent Web 2.0 concepts, service-oriented architecture, and Web-feed technologies.
Novel automated three‐dimensional genome scanning based on the nuclear architecture of telomeres (2010)

Telomeres, the end of chromosomes, are organized in a nonoverlapping fashion and form microterritories in nuclei of normal cells. Previous studies have shown that normal and tumor cell nuclei differ in their 3D telomeric organization. The differences include a change in the spatial organization of the telomeres, in telomere numbers and sizes and in the presence of telomeric aggregates. Previous attempts to identify the above parameters of 3D telomere organization were semi-automated. Here we describe the automation of 3D scanning for telomere signatures in interphase nuclei based on three-dimensional fluorescent in situ hybridization (3D-FISH) and, for the first time, define its sensitivity in tumor cell detection. The data were acquired with a high-throughput scanning/acquisition system that allows to measure cells and acquire 3D images of nuclei at high resolution with 40 × or 60 × oil and at a speed of 10,000-15,000 cells h(-1) , depending on the cell density on the slides. The automated scanning, TeloScan, is suitable for large series of samples and sample sizes. We define the sensitivity of this automation for tumor cell detection. The data output includes 3D telomere positions, numbers of telomeric aggregates, telomere numbers, and telomere signal intensities. We were able to detect one aberrant cell in 1,000 normal cells. In conclusions, we are able to detect tumor cells based on 3D architectural profiles of the genome. This new tool could, in the future, assist in patient diagnosis, in the detection of minimal residual disease, in the analysis of treatment response and in treatment decisions.
Nitric Oxide-Dependent Production of cGMP Supports the Survival of Rat Embryonic Motor Neurons Cultured with Brain-Derived Neurotrophic Factor (1998)

Trophic factor deprivation induces neuronal nitric oxide synthase (NOS) and apoptosis of rat embryonic motor neurons in culture. We report here that motor neurons constitutively express endothelial NOS that helps support the survival of motor neurons cultured with brain-derived neurotrophic factor (BDNF) by activating the nitric oxide-dependent soluble guanylate cyclase. Exposure of BDNF-treated motor neurons to nitro-L-arginine methyl ester (L-NAME) decreased cell survival 40-50% 24 hr after plating. Both low steady-state concentrations of exogenous nitric oxide (<0.1 microM) and cGMP analogs protected BDNF-treated motor neurons from death induced by L-NAME. Equivalent concentrations of cAMP analogs did not affect cell survival. Inhibition of nitric oxide-sensitive guanylate cyclase with 2 microM 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) reduced the survival of BDNF-treated motor neurons by 35%. cGMP analogs also protected from ODQ-induced motor neuron death, whereas exogenous nitric oxide did not. In all cases, cell death was prevented with caspase inhibitors. Our results suggest that nitric oxide-stimulated cGMP synthesis helps to prevent apoptosis in BDNF-treated motor neurons.
Donor/Acceptor Interactions in Systematically Modified Ru<sup>II</sup>−Os<sup>II</sup> Oligonucleotides (2002)

Donor/acceptor (D/A) interactions are studied in a series of doubly modified 19-mer DNA duplexes. An ethynyl-linked Ru(II) donor nucleoside is maintained at the 5' terminus of each duplex, while an ethynyl-linked Os(II) nucleoside, placed on the complementary strands, is systematically moved toward the other terminus in three base pair increments. The steady-state Ru(II)-based luminescence quenching decreases from 90% at the shortest separation of 16 A (3 base pairs) to approximately 11% at the largest separation of 61 A (18 base pairs). Time-resolved experiments show a similar trend for the Ru(II) excited-state lifetime, and the decrease in the averaged excited-state lifetime for each duplex is linearly correlated with the fraction quenched obtained by steady-state measurements. Analysis according to the Förster dipole-dipole energy transfer mechanism shows a reasonable agreement. Deviation from idealized behavior is primarily attributed to uncertainty in the orientation factor, kappa(2). Analyzing D/A interactions in an analogous series of doubly modified oligonucleotides, where the ethynyl-linked Ru(II) center is replaced with a saturated two-carbon linked complex, yields an excellent correlation with the Förster mechanism. As this simple change partially relaxes the rigid geometry of the donor chromophore, these results suggest that the deviation from idealized Förster behavior observed for the duplexes containing the rigidly held Ru(II) center originates, at least partially, from ambiguities in the orientation factor. Surprisingly, analyzing both quenching data sets according to the Dexter mechanism also shows an excellent correlation. Although this can be interpreted as strong evidence for a Dexter triplet energy transfer mechanism, it does not imply that this electron exchange mechanism is operative in these D/A duplexes. Rather, it suggests that systems that transfer energy via the Förster mechanism can under certain circumstances exhibit Dexter-like "behavior", thus illustrating the danger of imposing a single physical model to describe D/A interactions in such complex systems. While we conclude that the Förster dipole-dipole energy transfer mechanism is the dominant pathway for D/A interactions in these modified oligonucleotides, a minor contribution from the Dexter electron exchange mechanism at short distances is likely. This complex behavior distinguishes DNA-bridged Ru(II)/Os(II) dyads from their corresponding low molecular-weight and covalently attached counterparts.
Dopamine in vivo inhibits VEGF-induced phosphorylation of VEGFR-2, MAPK, and focal adhesion kinase in endothelial cells (2004)

Vascular permeability factor (VPF)/VEGF is a potent multifunctional cytokine and growth factor that has critical roles in vasculogenesis and in both physiological and pathological angiogenesis. Because it has been recently shown that the neurotransmitter dopamine at pharmacological dose can inhibit VEGF/VPF-mediated microvascular permeability, proliferation, and migration of endothelial cells in vitro, we therefore hypothesized that endogenous dopamine may regulate the actions of VPF/VEGF in vivo. We report that VPF/VEGF-induced phosphorylation of VEGF receptor 2, focal adhesion kinase, and MAPK in the endothelial cells is strikingly increased in both dopamine-depleted and dopamine D(2) receptor knockout mice compared with normal controls, thereby indicating that endogenous dopamine regulate these critical signaling cascades required for the in vivo endothelial functions of VPF/VEGF. Together, these observations provide new mechanistic insight into the dopamine-mediated inhibition of the activities of VPF/VEGF and suggest that endogenous neurotransmitter dopamine might be an important physiological regulator of VPF/VEGF activities in vivo.
Enzyme electrode for glucose based on the quinoprotein glucose dehydrogenase (1985)

Glucose dehydrogenase (E.C. 1.1.99.17) has been used to produce an amperometric enzyme electrode for glucose. With phenazine ethosulphate as the electron mediator, a linear response for up to 0.8 mM glucose was obtained with response times of 3–5 min. 2,6-Dichlorophenolindophenol as mediator gave long (> 30 min) response times, but the measurement time was reduced to 30 s by monitoring the first-order differential of the response; the derivatised signal allowed temporal separation of the enzyme-mediated signal from electrochemical interference. Whole blood glucose measurements were correlated with a spectrophotometric method based on glucose oxidase (y= 0.84x + 0.67, r= 0.963, n= 36).
Plasma displays (1991)

The author describes many of the various approaches which have been used in designing display devices. The emphasis is on device configuration and performance rather than on the physics of the glow discharge. There are three aspects of the physics the author mentions as being unusual to many plasma physicists: the use of priming in display-scanning mechanisms, the use of the Townsend discharge in a positive column for high output and efficacy, and the use of a double-layer sheath as a scannable source of electrons. The adroit use of priming can reduce the number of drive circuits required-an advantage unique in the display art to plasma devices. The gas discharge can be used as a source of electrons which can then excite cathodoluminescent phosphors in a variety of colors. It can also be used as a selection means for liquid-crystal displays. A wide variety of device configurations, using both unidirectional and bidirectional pulse excitations, is described.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Industrial applications of ultrahigh precision short-pulse laser processing (Invited Paper) (2005)

Short-pulse lasers of femtosecond and picosecond durations are used in ultrahigh precision processing of optical, electronic, and micro-mechanical devices. In this paper we discuss the fabrication of high quality photonic crystal band-pass filters with femtosecond laser pulses. We also discuss the parallel processing of inkjet nozzles with a picosecond laser. A laser milling algorithm and a high accuracy beam scanner allows the formation of precisely shaped holes as inkjet nozzles, and an efficient diffractive beam splitter allows the simultaneous drilling of many hundreds of holes for low cost manufacturing.
Cell adhesion and metastasis (1992)

There is no single phenotypic trait whose exclusive expression correlates universally with metastatic behaviour. Nevertheless, several lines of evidence point towards adhesive phenomena as mediating some crucial steps in the malignant spread of particular tumour types. These steps include detachment from the primary, invasion of the ECM including include detachment from the primary, invasion of the ECM including structures such as the basement membrane and organ capsules, and lodgement in the vessels of remote organs. Furthermore, there is evidence that adhesive phenomena can contribute at least in part to the selective patterns of spread shown by a variety of tumour cell types. The molecular dissection of the adhesive events involved in these processes has only just begun. It is clear already, however, that several different determinants are likely to be involved and the clinical application of this knowledge is unlikely to be immediate.
Domain Analysis of Protein P30 in <i>Mycoplasma pneumoniae</i> Cytadherence and Gliding Motility (2011)

The cell wall-less prokaryote Mycoplasma pneumoniae causes bronchitis and atypical pneumonia in humans. Mycoplasma attachment and gliding motility are required for colonization of the respiratory epithelium and are mediated largely by a differentiated terminal organelle. P30 is a membrane protein at the distal end of the terminal organelle and is required for cytadherence and gliding motility, but little is known about the functional role of its specific domains. In the current study, domain deletion and substitution derivatives of P30 were engineered and introduced into a P30 null mutant by transposon delivery to assess their ability to rescue P30 function. Domain deletions involving the extracellular region of P30 severely impacted protein stability and adherence and gliding function, as well as the capacity to stabilize terminal organelle protein P65. Amino acid substitutions in the transmembrane domain revealed specific residues uniquely required for P30 stability and function, perhaps to establish correct topography in the membrane for effective alignment with binding partners. Deletions within the predicted cytoplasmic domain did not affect P30 localization or its capacity to stabilize P65 but markedly impaired gliding motility and cytadherence. The larger of two cytoplasmic domain deletions also appeared to remove the P30 signal peptide processing site, suggesting a larger leader peptide than expected. We propose that the P30 cytoplasmic domain may be required to link P30 to the terminal organelle core, to enable the P30 extracellular domain to achieve a functional conformation, or perhaps both.
Chemical Synthesis of Modified RNA (2010)

This chapter contains sections titled: Introduction The chemical synthesis of modified RNA Examples of modified RNA for the analysis and manipulation of RNA secondary and tertiary structures Conclusion Acknowledgments References
Comparison of continuous and intermittent renal replacement therapy for acute renal failure (2005)

The present investigation provides no evidence for a survival benefit of continuous vs intermittent RRT in ICU patients with ARF.
Hypervitaminosis A: side-effects of retinoids (1986)

Conference Article| October 01 1986 Hypervitaminosis A: side-effects of retinoids ANDREAS KISTLER ANDREAS KISTLER 1Biological Pharmaceutical Research Department, F. Hoffmann-La Roche & Co. Ltd., CH-4002 Basle, Switzerland Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1986) 14 (5): 936–939. https://doi.org/10.1042/bst0140936 Article history Received: May 06 1986 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation ANDREAS KISTLER; Hypervitaminosis A: side-effects of retinoids. Biochem Soc Trans 1 October 1986; 14 (5): 936–939. doi: https://doi.org/10.1042/bst0140936 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search © 1986 Biochemical Society1986 Article PDF first page preview Close Modal You do not currently have access to this content.
Influence of surgically induced varicocele on testicular blood flow, temperature, and histology in adult rats and dogs. (1981)

Varicocele had been repeatedly implicated as a cause of infertility in selected men, although neither a causal relationship nor a mechanism has been documented. The purpose of this investigation was to create a varicocele model in animals and to study the subsequent alterations in testicular physiology. Secondary dilatation of the left internal spermatic vein was achieved either by partial ligation of the left renal vein in rats and dogs or by surgical destruction of the valve of the left testicular vein in a second group of dogs. 1 mo after partial ligation in the rats and 3 mo after partial ligation or valve destruction in the dogs, testicular blood flow was measured using Strontium 85 (SR-85)-labeled microspheres (15 +/- 1.1 micrometer). Intratesticular temperature was measured with a Bailey needle probe thermometer and biopsies were obtained for histologic sections. Mean testicular blood flow in milliliters per minute per 100 g was significantly greater in the partially ligated rats; right testis control 26 +/- 2, left testis control 24 +/- 2 compared to right testis experimental 35 +/- 3, left testis experimental 35 +/- 4 (p less than 0.02). Dogs undergoing either partial vein ligation or valve destruction showed similar increases in mean testicular blood flow; right testis control 8 +/- 1, left testis control 8 +/- 1 vs. right testis experimental 16 +/- 3, left testis experimental 18 +/- 4 (p less than 0.01). The mean difference between intratesticular and intraperitoneal temperature in control rats was significantly higher (4.02 +/- 0.25 degrees C right testis, 3.77 +/- 0.14 degrees C left testis), than rats who underwent partial vein ligation (right testis 2.14 +/- 0.09 degrees C, left testis 2.34 +/- 0.12 degrees C) (p less than 0.001). Control dogs also had a significantly higher mean difference between intratesticular and rectal temperatures; (right testis control 3.61 +/- 0.42 degrees C, left testis control 3.60 +/- 0.40 degrees C) than the partially ligated or valve destruction dogs (right testis 2.31 +/- 0.17 degrees C, left testis 2.67 +/- 0.32 degrees C) (p less than 0.05). In addition, histologic evaluation revealed abnormalities in spermatogenesis in some of the animals. Thus, venous dilatation secondary to partial vein ligation or testicular vein valve obliteration is followed by large bilateral increases in testicular blood flow in these two species. A consequence of this increased flow is an elevation in bilateral testicular temperature, which it is postulated, leads to impaired spermatogenesis in some of the animals. In selected men varicocele may impair spermatogenesis by a similar physiologic mechanism.
5,5″-Disubstituted 2,2′:6′,2″-Terpyridines through and for Metal-Mediated Cross-Coupling Chemistry (1999)

A novel functionality pattern for terpyridines results from the introduction of halogen, stannyl, and boronic ester functionalities at positions C-5 and C-5″ (see figure). This considerably widens the applicability of this class of tridentate ligands for supra- and macromolecular applications. The feasibility of growth reactions with these novel terpyridines is demonstrated.
Epidemiology of DSM-III-R major depression and minor depression among adolescents and young adults in the national comorbidity survey (1998)

Data on the prevalences, comorbidities, and cohort effects of DSM-III-R major depression (MD) and minor depression (mD) are reported for the nationally representative sample of n = 1,769 adolescents and young adults who participated in the National Comorbidity Survey. Lifetime prevalences are 15.3% (MD) and 9.9% (mD), while 30-day prevalences are 5.8% (MD) and 2.1% (mD). Most cases reported recurrent episodes (73.9% of those with MD and 69.2% with mD) and significant role impairment, including attempted suicide among 21.9% of those with MD. The majority of lifetime cases (76.7% of those with MD and 69.3% with mD) reported other comorbid lifetime NCS/ DSM-III-R disorders. Depression was temporally secondary in the majority of these cases. Number of prior disorders was more important than type of disorders in predicting subsequent depression, raising the possibility that secondary depression is a nonspecific severity marker for earlier disorders. A cohort effect for both MD and mD was documented that persisted even for episodes lasting a year or longer. Increasing prevalences of prior comorbid disorders were found to play an important part in explaining the cohort effect for depression.
Homeostatic Responses to Prolonged Cold Exposure: Human Cold Acclimatization (1996)

The sections in this article are: 1 Naturally Occurring Cold Acclimatization 1.1 Circumpolar Residents 1.2 Primitive People Living in Temperate-Weather Regions 1.3 Modern People Repeatedly lmmersed in Cold Water 2 Experimentally Induced Cold Acclimation 2.1 Acclimation Induced by Repeated Cold-Air Exposure 2.2 Acclimation Induced by Repeated Cold-Water Immersion 3 Determinants of the Acclimatization Pattern 4 Summary
A Fully Compressible Three Dimensional Inverse Design Method Applicable to Radial and Mixed Flow Turbomachines (1990)

A fully three dimensional compressible inverse design method for the design of radial and mixed flow machines is described. In this method the distribution of the circumferentially averaged swirl velocity, or rV¯θ on the meridional geometry of the impeller is prescribed and the corresponding blade shape is computed iteratively. Two approaches are presented for solving the compressible flow problem. In the approximate approach, the pitchwise variation in density is neglected and as a result the algorithm is simple and efficient. In the exact approach, the velocities and density are computed throughout the three dimensional flow field by employing Fast Fourier Transform in the tangential direction. The results of the approximate and exact approach are compared for the case of a high speed (subsonic) radial-inflow turbine and it is shown that the difference between the blade shapes computed by the two methods is well within the manufacturing tolerances. The flow through the designed impeller is analysed by using three dimensional inviscid and viscous time marching programs and very good correlations between the specified and computed rV¯θ is obtained.