gebhart/patrepeval huggingface dataset:
Computer‐controlled vacuum backflush for capillary GC (1990)

Abstract A vacuum‐pump‐operated backflush system has been developed for applications involving high‐speed, repetitive GC analysis of gas streams containing ppm and ppb levels of organic vapor. The system uses an injector capable of cryofocusing and a relatively short length of 0.25‐mm i.d. fused silica column for the separation of relatively simple mixtures of volatile compounds. Analysis times typically are in the 5–10 s range, and backflush times are in the 2–5 s range. Gases from the flame ionization detector are used as the backflush carrier gas, and no modification of the detector is necessary. A procedure is described which allows the average gas velocity to be measured during backflush operation. The minimum backflush time is directly proportional to the analysis time and to the square of the column length, making this system most useful with short columns and short analysis times.
Characterization of CCD sensor for actinic mask blank inspection (2006)

Back-illuminated charge-coupled devices (BI-CCD) have been characterized for the application of actinic EUVL mask blank inspection. Point spread function (PSF) of a BI-CCD is analyzed by extracting the EUV-emulating events from photon counting images of <sup>55</sup>Fe exposure experiments. The spread of the PSF is found to be reduced by applying higher voltage to extend the depletion layer, which is consistent with the physical model of the electron diffusion in the backside region. High speed capability of the most promising BI-CCD is also tested to investigate its applicability to production-worthy high throughput inspection tools. It has been demonstrated that a clear image can be obtained at 5.6MHz clock speed which translates into the throughput of 2 hours per blank using 26x imaging optics. The readout noise at this clock speed, however, has turned out to be extremely degraded than is required to keep the required sensitivity. Potential approaches to overcome sensitivity degradation caused by the increase in the readout noise are discussed.
Who Is Mr. HAMLET? Interaction of Human α-Lactalbumin with Monomeric Oleic Acid (2008)

A specific state of the human milk Ca(2+) binding protein alpha-lactalbumin (hLA) complexed with oleic acid (OA) prepared using an OA-pretreated ion-exchange column (HAMLET) triggers several cell death pathways in various tumor cells. The possibility of preparing a hLA-OA complex with structural and cytotoxic properties similar to those of the HAMLET but under solution conditions has been explored. The complex was formed by titration of hLA by OA at pH 8.3 up to OA critical micelle concentration. We have shown that complex formation strongly depends on calcium, ionic strength, and temperature; the optimal conditions were established. The spectrofluorimetrically estimated number of OA molecules irreversibly bound per hLA molecule (after dialysis of the OA-loaded preparation against water followed by lyophilization) depends upon temperature: 2.9 at 17 degrees C (native apo-hLA; resulting complex referred to as LA-OA-17 state) and 9 at 45 degrees C (thermally unfolded apo-hLA; LA-OA-45). Intrinsic tryptophan fluorescence measurements revealed substantially decreased thermal stability of Ca(2+)-free forms of HAMLET, LA-OA-45, and OA-saturated protein. The irreversibly bound OA does not affect the Ca(2+) association constant of the protein. Phase plot analysis of fluorimetric and CD data indicates that the OA binding process involves several hLA intermediates. The effective pseudoequilibrium OA association constants for Ca(2+)-free hLA were estimated. The far-UV CD spectra of Ca(2+)-free hLA show that all OA-bound forms of the protein are characterized by elevated content of alpha-helical structure. The various hLA-OA complexes possess similar cytotoxic activities against human epidermoid larynx carcinoma cells. Overall, the LA-OA-45 complex possesses physicochemical, structural, and cytotoxic properties closely resembling those of HAMLET. The fact that the HAMLET-like complex can be formed in aqueous solution makes the process of its preparation more transparent and controllable, opening up opportunities for formation of active complexes with specific properties.
Nitrogen-15 NMR study of [15N]actinomycin D complexed with d(pGpC) and DNA (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNitrogen-15 NMR study of [15N]actinomycin D complexed with d(pGpC) and DNAStephen C. Brown, Richard H. Shafer, and Peter A. MirauCite this: J. Am. Chem. Soc. 1982, 104, 20, 5504–5506Publication Date (Print):October 1, 1982Publication History Published online1 May 2002Published inissue 1 October 1982https://pubs.acs.org/doi/10.1021/ja00384a050https://doi.org/10.1021/ja00384a050research-articleACS PublicationsRequest reuse permissionsArticle Views54Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Killing the Messenger: Antisense DNA and siRNA (2004)

Among the technologies available for gene knockdown RNase H-dependent antisense oligonucleotides and RNAi are very popular. Both offer specificity and efficient knockdown of the genes; both are useful tools to study gene functions. Antisense and RNAi methods share many practical problems such as site selection, toxicity at high concentration, and the difficulty of transfection in certain cell types. We will focus in this review on the most important issues in the development of both methods and their possible use in gene-silencing therapy.
Surface De-PEGylation Controls Nanoparticle-Mediated siRNA Delivery <i>In Vitro</i> and <i>In Vivo</i> (2017)

The present work proposes a unique de-PEGylation strategy for controllable delivery of small interfering RNA (siRNA) using a robust lipid-polymer hybrid nanoparticle (NP) platform. The self-assembled hybrid NPs are composed of a lipid-poly(ethylene glycol) (lipid-PEG) shell and a polymer/cationic lipid solid core, wherein the lipid-PEG molecules can gradually dissociate from NP surface in the presence of serum albumin. The de-PEGylation kinetics of a series of different lipid-PEGs is measured with their respective NPs, and the NP performance is comprehensively investigated <i>in vitro</i> and <i>in vivo</i>. This systematic study reveals that the lipophilic tails of lipid-PEG dictate its dissociation rate from NP surface, determining the uptake by tumor cells and macrophages, pharmacokinetics, biodistribution, and gene silencing efficacy of these hybrid siRNA NPs. Based on our observations, we here propose that lipid-PEGs with long and saturated lipophilic tails might be required for effective siRNA delivery to tumor cells and gene silencing of the lipid-polymer hybrid NPs after systemic administration.
Severe <scp>SARS‐CoV</scp>‐2 infection in a 32‐week pregnant woman treated with Remdesivir‐Dexamethasone combination therapy: A case report (2022)

This study shows that remdesivir and dexamethasone combination therapy can be considered as a suitable treatment choice for pregnant women infected with COVID-19. It is worth mentioning that more studies are required to evaluate the efficacy and side effects of remdesivir monotherapy and its combination with dexamethasone during pregnancy.
<i>LEUNIG</i> regulates <i>AGAMOUS</i> expression in <i>Arabidopsis</i> flowers (1995)

LEUNIG was identified in a genetic screen designed to isolate second-site enhancer mutations of the floral homeotic mutant apetala2-1. leunig mutations not only enhance apetala2, but by themselves cause a similar but less-pronounced homeotic transformation than apetala2 mutations. leunig flowers have sepals that are transformed toward stamens and carpels, and petals that are either staminoid or absent. In situ hybridization experiments with leunig mutants revealed altered expression pattern of the floral homeotic genes APETALA1, APETALA3, PISTILLATA, and AGAMOUS. Double mutants of leunig and agamous exhibited a phenotype similar to agamous single mutants, indicating that agamous is epistatic to leunig. Our analysis suggests that a key role of LEUNIG is to negatively regulate AGAMOUS expression in the first two whorls of the Arabidopsis flower.
Pyruvate kinase M2 regulates glucose metabolism by functioning as a coactivator for hypoxia-inducible factor 1 in cancer cells (2011)

Cancer cells feature altered glucose metabolism that allows their rapid growth. They consume large amounts of glucose to produce lactate, even in the presence of ample oxygen, which is known as the Warburg effect. Pyruvate kinase M2 (PKM2) contributes to the Warburg effect by previously unknown mechanisms. Hypoxia-inducible factor 1 (HIF-1) mediates PKM2 gene transcription and glucose reprogramming in cancer cells. The recent discovery of novel physical and functional interactions between PKM2 and HIF-1 in cancer cells has provided insight into molecular mechanisms underlying the Warburg effect.
Isochromophilones III -VI, Inhibitors of Acyl-CoA: Cholesterol Acyltransferase Produced by Penicillium multicolor FO-3216. (1995)

New azaphilones named isochromophilones III-VI were isolated from the culture broth of Penicillium multicolor FO-3216 as inhibitors of acyl-CoA: cholesterol acyltransferase (ACAT). Their structures were elucidated by NMR and other spectroscopic analyses. The IC50 values of isochromophilones III, IV, V and VI for ACAT activity in an enzyme assay using rat liver microsomes were calculated to be 110, 50, 50 and 120 microM, respectively.
SPLITTING THE SCENE GRAPH - Using Spatial Relationship Graphs Instead of Scene Graphs in Augmented Reality (2008)

Scene graphs have been a core element of 3D graphics since the publication of Inventor. However, in Virtual and Augmented Reality applications, 3D graphics are often interleaved with and controlled by real-world data provided by pose trackers, cameras and other kinds of sensors. In such a setup, the generalized concept of a Spatial Relationship Graph (SRG) might be better suited as an underlying data structure to describe the application and its components. In this paper, we will give an overview of the SRG concept, describe its difference to a scene graph and provide an example AR application built upon an SRG-based tracking library.
Suppression of progenitor differentiation requires the long noncoding RNA ANCR (2012)

Long noncoding RNAs (lncRNAs) regulate diverse processes, yet a potential role for lncRNAs in maintaining the undifferentiated state in somatic tissue progenitor cells remains uncharacterized. We used transcriptome sequencing and tiling arrays to compare lncRNA expression in epidermal progenitor populations versus differentiating cells. We identified ANCR (anti-differentiation ncRNA) as an 855-base-pair lncRNA down-regulated during differentiation. Depleting ANCR in progenitor-containing populations, without any other stimuli, led to rapid differentiation gene induction. In epidermis, ANCR loss abolished the normal exclusion of differentiation from the progenitor-containing compartment. The ANCR lncRNA is thus required to enforce the undifferentiated cell state within epidermis.
Model-Driven Management of Docker Containers (2016)

With the emergence of Docker, it becomes easier to encapsulate applications and their dependencies into lightweight Linux containers and make them available to the world by deploying them in the cloud. Compared to hypervisor-based virtualization approaches, the use of containers provides faster start-ups times and reduces the consumption of computer resources. However, Docker lacks of deployability verification tool for containers at design time. Currently, the only way to be sure that the designed containers will execute well is to test them in a running system. If errors occur, a correction is made but this operation can be repeated several times before the deployment becomes operational. Docker does not provide a solution to increase or decrease the size of container resources in demand. Besides the deployment of containers, Docker lacks of synchronization between the designed containers and those deployed. Moreover, container management with Docker is done at low level, and therefore requires users to focus on low level system issues. In this paper we focus on these issues related to the management of Docker containers. In particular, we propose an approach for modeling Docker containers. We provide tooling to ensure the deployability and the management of Docker containers. We illustrate our proposal using an event processing application and show how our solution provides a significantly better compromise between performance and development costs than the basic Docker container solution.
Estrogen Receptor-Mediated Actions of Polyphenolic Catechins in Vivo and in Vitro (2002)

Recent investigations have demonstrated that polyphenolic catechins inhibit breast cancer cell proliferation and tumor growth. However, the ER-mediated effects of the three predominant catechins (EGCG, ECG, and EGC) have not been extensively examined in vitro or in vivo. Therefore, EGCG, ECG, and EGC were examined for their ability to compete with [(3)H]-17beta-estradiol ([(3)H]-E(2)) for binding to ERalpha and ERbeta and to elicit reporter gene activity in MCF-7 human breast cancer cells transiently transfected with either chimeric ERalpha or ERbeta. EGCG and ECG displaced [(3)H]-E(2) from GST-hERalphadef (D, E, and F domains of human ERalpha fused to GST) or from full-length human ERbeta. Additionally, only EGCG elicited Gal4-hERalphadef and Gal4-mERbetadef-mediated reporter gene expression (EC(50) values: 28 and 19 micro M, respectively) in MCF-7 cells cotransfected with a Gal4-regulated luciferase reporter gene. In cotreatment experiments, EGCG (1-50 micro M) and ECG (1 micro M) decreased E(2)-induced (1 nM) ERbeta-mediated gene expression 35-50%. In vivo, no catechin induced estrogenic responses (uterine weight or uterine peroxidase activity) in immature C57BL/6 mice. However, when mice were cotreated with E(2) (10 micro g/kg/day, 3 days) and either EGCG (30 and 50 mg/kg/day, 3 days) or ECG (50 mg/kg/day, 3 days), uterine peroxidase activity was increased 2.3-fold above that elicited by E(2) alone. In conclusion, EGCG and ECG bind to ERalpha and ERbeta, but only EGCG elicited ER-mediated gene expression in vitro. However, both of these compounds moderately increased E(2)-inducible responses in vivo.
Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2 (2001)

Many tumor cells are impaired in adhesion-regulated apoptosis, which contributes to their metastatic potential. However, suppression of this apoptotic pathway in untransformed cells is not mediated only by adhesion to the extracellular matrix but also through the resulting ability to spread and adopt a distinct morphology. Since cell spreading is dependent on the integrity of the actin microfilament cytoskeleton, we sought to determine if actin depolymerization was sufficient to induce apoptosis, even in the presence of continuous attachment. For this study, we used a human mammary epithelial cell line (MCF10A), which is immortalized but remains adhesion dependent for survival. Treatment of MCF10A cells with latrunculin-A (LA), an inhibitor of actin polymerization, rapidly led to disruption of the actin cytoskeleton and caused cell rounding but preserved attachment. Initiation of apoptosis in LA-treated MCF10A cells was detected by mitochondrial localization of the Bax apoptotic protein, which was prevented by overexpression of Bcl-2. DNA fragmentation and poly(ADP-ribose) polymerase (PARP) cleavage in LA-treated MCF10A cells indicated progression to the execution phase of apoptosis. The MDA-MB-453 cell line, which was derived from a metastatic human mammary tumor, was resistant to PARP cleavage and loss of viability in response to actin depolymerization. Stable overexpression of Bcl-2 in the untransformed MCF10A cells was able to recapitulate the resistance to apoptosis found in the tumor cell line. We demonstrate that inhibition of actin polymerization is sufficient to stimulate apoptosis in attached MCF10A cells, and we present a novel role for Bcl-2 in cell death induced by direct disruption of the actin cytoskeleton.
Low-Cost Automation for Aircraft Assembly (2004)

<div class="htmlview paragraph">In this paper solution for low-cost automation of aircraft assembly is presented. The concept of this development is closely related to “Lean Automation”, which in this case concerns the use of modern standard equipment such as standard robots, PC-computers and a newly developed spatial sensor system for precision measurements of positions. The robot is used to perform reconfiguration of tooling modules that are possible to be configured/reconfigured in six degrees of freedom. A prototype developed as the result of an EU-project called ADFAST* has been evaluated at Linköping University in Sweden. Technical functionality is reported where the robot manages to configure the flexible tooling modules to a total error bellow 50 μm. This paper presents the results on the portion of the project addressing robot, metrology system and tooling.</div>
L(+)-lactate dehydrogenase in sputum from patients with chronic obstructive lung diseases. (1972)

Abstract Human sputum specimens can be handled and homogenized like a tissue. Study of the stereochemical specificity of lactate dehydrogenase at pH 9.3, has demonstrated that substantial amount of l(+)-Lactate-dependent activities exist in sputa of patients with chronic obstructive lung diseases. d(−)-Lactate dehydrogenase activities either were absent or present in very low concentrations. The l(+)-Lactate dehydrogenase activities of human sputum appear to be primarily of the heart (H) type which requires optimal low concentrations of pyruvate for maximal activity. The sputum enzyme has characteristics similar to that found previously for human whole saliva. The primary source of the enzyme in human whole saliva is believed to be the polymorphonuclear leukocyte which is also a major cell type found in human sputum specimens. The activities of whole sputum homogenates have been compared to those of sputum homogenate supernatant extracts, and the activities were found to be virtually the same. Freezing and thawing of whole homogenates resulted in a variable loss of enzyme activity.
The use of poly(alkylene oxide)s to achieve fast and controlled photochromic switching in rigid matrices (2012)

Abstract We report a conjugation system for the enhancement of photochromic dye performance in rigid matrices using widely available, cheap, chemically robust and compatible polymeric starting materials, namely poly(propylene oxide) (PPO) and poly(1,2‐butylene oxide). Conjugation of these soft (low T g ) polymers to an indeno‐fused naphthopyran photochromic dye, in a telechelic geometry, gives access to a wide range of accelerated and tuned fade speeds (decoloration) via variation in molecular weight. The t 1/2 and t 3/4 fade speeds for PPO conjugates (polymer molecular weights ranging between ca. 425 and 2000) are accelerated by 35–58 and 51–76%, respectively, compared with the nonconjugated control dye. Longer oligomers provide faster decoloration approaching that obtained in solution. The stability of the polyethers allows functionalization using a wide variety of chemistries, including harsh acid catalyzed transformations, providing an overall facile synthesis of photochromic dye‐polymer conjugates in high yield and purity. In addition, these polymers give easy access to conjugates with star‐type architectures, which provide an even further improvement in performance compared with their linear counterparts with less conjugated polymer needed per dye to achieve a given fade speed. © 2012 Commonwealth of Australia. J Polym Sci Part A: Polym Chem, 2012
The purification of cholinesterase from horse serum (1974)

A relatively simple method is described by which cholinesterase was purified about 19000-fold starting from horse serum. Typically 20 litres of serum were processed to yield 15-18mg of electrophoretically pure cholinesterase in the form of an active salt-free dry powder. The method included two stages: fractionation with (NH(4))(2)SO(4) and ion-exchange chromatography. The (NH(4))(2)SO(4) stage included, in principle, the acid (pH3) step of the Strelitz (1944) procedure. The step took advantage of the stabilizing effect that 33%-satd. (NH(4))(2)SO(4) has on cholinesterase activity at pH3 and it is recognized that in the absence of (NH(4))(2)SO(4) the enzyme is rapidly destroyed at pH3. Cholinesterase was significantly more stable to pH3.0 at 2 degrees C than at 24 degrees C, and the acid step was done at both temperatures. The specific activities of the final products obtained by way of acid steps were the same at either temperature, thus indicating that the step has not harmed the enzyme active sites. The product from the first two stages was purified over 18000-fold and was 85-90% cholinesterase. The remaining impurities were removed by preparative gel electrophoresis. The product was about 40% more active and contained 40% more active sites per unit weight than electrophoretically pure cholinesterase prepared from partially purified commercial starting material. Although the number of active sites per molecule was not determined with certainty, a value of at least 3 and possibly 4 was indicated. The partial specific volumes were determined with a precision density meter, on the ultracentrifuge and from the amino acid and carbohydrate composition. The values by these independent methods were 0.688, 0.71 and 0.712ml/g, respectively. The amino acid and carbohydrate composition was determined. The cholinesterase contained 17.4% carbohydrate including 3.2% N-acetylneuraminic acid.
Simulation tool for the assessment of adaptive techniques to improve the TETRA air interface (2000)

This paper presents an evaluation system for the dynamic control of TETRA radio systems. Detailed link-level simulation results are controlled by event driven system level simulations which allows the operation of the overall system to be emulated accurately but with relatively low computational overhead. The system is particularly useful for studying the behaviour of adaptive algorithms when the system is in a transition between different states, and for predicting the quality of service delivered to the end user. (6 pages)
Computational approaches for generating electromagnetic Gaussian Schell-model sources (2014)

Two different methodologies for generating an electromagnetic Gaussian-Schell model source are discussed. One approach uses a sequence of random phase screens at the source plane and the other uses a sequence of random complex transmittance screens. The relationships between the screen parameters and the desired electromagnetic Gaussian-Schell model source parameters are derived. The approaches are verified by comparing numerical simulation results with published theory. This work enables one to design an electromagnetic Gaussian-Schell model source with pre-defined characteristics for wave optics simulations or laboratory experiments.