gebhart/patrepeval huggingface dataset:
Temperature-, concentration- and cholesterol-dependent translocation of <scp>L</scp>- and <scp>D</scp>-octa-arginine across the plasma and nuclear membrane of CD34+ leukaemia cells (2007)

Delineating the mechanisms by which cell-penetrating peptides, such as HIV-Tat peptide, oligoarginines and penetratin, gain access to cells has recently received intense scrutiny. Heightened interest in these entities stems from their ability to enhance cellular delivery of associated macromolecules, such as genes and proteins, suggesting that they may have widespread applications as drug-delivery vectors. Proposed uptake mechanisms include energy-independent plasma membrane translocation and energy-dependent vesicular uptake and internalization through endocytic pathways. In the present study, we investigated the effects of temperature, peptide concentration and plasma membrane cholesterol levels on the uptake of a model cell-penetrating peptide, L-octa-arginine (L-R8) and its D-enantiomer (D-R8) in CD34+ leukaemia cells. We found that, at 4-12 degrees C, L-R8 uniformly labels the cytoplasm and nucleus, but in cells incubated with D-R8 there is additional labelling of the nucleolus which is still prominent at 30 degrees C incubations. At temperatures between 12 and 30 degrees C, the peptides are also localized to endocytic vesicles which consequently appear as the only labelled structures in cells incubated at 37 degrees C. Small increases in the extracellular peptide concentration in 37 degrees C incubations result in a dramatic increase in the fraction of the peptide that is localized to the cytosol and promoted the binding of D-R8 to the nucleolus. Enhanced labelling of the cytosol, nucleus and nucleolus was also achieved by extraction of plasma membrane cholesterol with methyl-beta-cyclodextrin. The data argue for two, temperature-dependent, uptake mechanism for these peptides and for the existence of a threshold concentration for endocytic uptake that when exceeded promotes direct translocation across the plasma membrane.
Temporal analysis of text data using latent variable models (2009)

Detecting and tracking of temporal data is an important task in multiple applications. In this paper we study temporal text mining methods for music information retrieval. We compare two ways of detecting the temporal latent semantics of a corpus extracted from Wikipedia, using a stepwise probabilistic latent semantic analysis (PLSA) approach and a global multiway PLSA method. The analysis indicates that the global analysis method is able to identify relevant trends which are difficult to get using a step-by-step approach. Furthermore we show that inspection of PLSA models with different number of factors may reveal the stability of temporal clusters making it possible to choose the relevant number of factors.
Mass Spectrometry (1971)

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.
Short-Term Temporal Discounting of Reward Value in Human Ventral Striatum (2009)

Delayed rewards lose their value for economic decisions and constitute weaker reinforcers for learning. Temporal discounting of reward value already occurs within a few seconds in animals, which allows investigations of the underlying neurophysiological mechanisms. However, it is difficult to relate these mechanisms to human discounting behavior, which is usually studied over days and months and may engage different brain processes. Our study aimed to bridge the gap by using very short delays and measuring human functional magnetic resonance responses in one of the key reward centers of the brain, the ventral striatum. We used psychometric methods to assess subjective timing and valuation of monetary rewards with delays of 4.0-13.5 s. We demonstrated hyperbolic and exponential decreases of striatal responses to reward predicting stimuli within this time range, irrespective of changes in reward rate. Lower reward magnitudes induced steeper behavioral and striatal discounting. By contrast, striatal responses following the delivery of reward reflected the uncertainty in subjective timing associated with delayed rewards rather than value discounting. These data suggest that delays of a few seconds affect the neural processing of predicted reward value in the ventral striatum and engage the temporal sensitivity of reward responses. Comparisons with electrophysiological animal data suggest that ventral striatal reward discounting may involve dopaminergic and orbitofrontal inputs.
Ultra-High Input Impedance, Low Noise Integrated Amplifier for Noncontact Biopotential Sensing (2011)

Noncontact electrocardiogram/electroencephalogram/ <?Pub _bookmark="" Command="[Quick Mark]"?> electromyogram electrodes, which operate primarily through capacitive coupling, have been extensively studied for unobtrusive physiological monitoring. Previous implementations using discrete off-the-shelf amplifiers have been encumbered by the need for manually tuned input capacitance neutralization networks and complex dc-biasing schemes. We have designed and fabricated a custom integrated noncontact sensor front-end amplifier that fully bootstraps internal and external parasitic impedances. DC stability without the need for external large valued resistances is ensured by an ac bootstrapped, low-leakage, on-chip biasing network. The amplifier achieves, without neutralization, input impedance of 60 fF <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\Vert$</tex></formula> 50 T <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\Omega$</tex></formula> , input referred noise of 0.05 fA/ <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\sqrt{\rm Hz}$</tex></formula> and 200 nV/ <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\sqrt{\rm Hz}$</tex></formula> at 1 Hz, and power consumption of 1.5 <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$\mu$</tex></formula> A per channel at 3.3 V supply voltage. Stable frequency response is demonstrated below 0.05 Hz with electrode coupling capacitances as low as 0.5 pF.
[Aerobic upper body training. An underutilized potential in training therapy]. (1992)

Until recently, there was little or no emphasis on aerobic upper body exercise training, despite research strongly supports the inclusion of this type of exercise in cardiac rehabilitation programs and adult fitness. The limited degree of transfer of training benefits from trained limbs to another set of untrained limbs appears to discount the practice of recommending aerobic leg exercise alone. In addition, leg training programs fail to accommodate individuals with specific health problems. There is an increased physiological stress during arm training, if compared to leg training. Exercise intensity derived from a bicycle- or treadmill test would therefore cause an inappropriately target heart rate for upper body exercise. Thus, target heart rate has to be reduced by 10 to 20% to be adequate for aerobic arm training. Finally, an example of a progressive arm exercise training program is shown.
ATTENUATION OF COHERENT NOISE IN MARINE SEISMIC EXPLORATION USING VERY LONG ARRAYS * (1978)

A bstract The use of arrays to separate primary reflections from unwanted coherent seismic events is common practice in land seismic surveys. Very long source and receiver arrays have been used recently to reduce the effects of waterbottom multiples on marine seismic data. The source array consists of five uniformly spaced identical subarrays, each with five different airguns, where the distance between the subarrays may vary from 20 m56 m. The volume of each subarray is 10.3 1 (630 cu.in.) which gives a total volume of the array of 51.5 1 (3150 cu.in.) operated at a pressure of 14 MPa (2000 psi). In order to have a flexible receiver system it was decided to implement the extended receiver array in data processing by computing a weighted sum of two to five traces. The hydrophone cable consists of fifty‐four channels with a group length of 50 m. Data shot with the superlong airgun array are processed by a combination of standard techniques and special procedures. In particular, the quality of the stack section is improved by using a weighted stack. The stack weights are computed by a program which takes into account the primary‐to‐multiple ratio. Comparisons with conventional data show significant improvements in data quality obtained by using the superlong airgun array. Examples show that the waterbottom multiples have been strongly attenuated and the deep seismic events have been enhanced. The combined array response function for dipping events is given in an appendix.
Molecular Hydrogen: New Antioxidant and Anti-inflammatory Therapy for Rheumatoid Arthritis and Related Diseases (2013)

Rheumatoid arthritis (RA) is a chronic inflammatory disease in which the progressive destruction of joint causes morbidity. It is also associated with an increased risk of atherosclerosis, which can result in cardiovascular disease and mortality. The therapeutic goal is to control the systemic inflammation to obtain not only the remission of symptoms, but also improve general state of health. Although recent biologic immunosuppressive therapies targeting pro-inflammatory cytokines have spawned a paradigm shift regarding the prognosis of RA, these therapies possess inherent side effects. Also, early diagnosis of the disease remains confounded by uncertainty. While the mechanisms responsible for the onset of RA remain unclear, reactive oxygen species (ROS) play a significant role in the pathogenesis of RA. ROS play a central role both upstream and downstream of NF-κB and TNFα pathways, which are located at the center of the inflammatory response. Among the ROS, the hydroxyl radical is the most harmful, and molecular hydrogen (H2) is a selective scavenger for this species. Recently, it has been shown that H2 is useful when administered along with the conventional therapy in RA as it acts to reduce oxidative stress in the patients. Especially in the early stage, H2 showed significant therapeutic potential, which also seemed to assist diagnosis and treatment decisions of RA. The possible expectations regarding the potential benefits of H2 by reducing the oxidative stress, resulting from inflammatory factors, are raised and discussed here. They include prevention of RA and related atherosclerosis, as well as therapeutic validity for RA.
Engineering aspects of disruption current decay (1983)

Engineering features associated with the configuration of a tokamak can affect the amount of energy that produces melting and damage to the limiters or internal wall surfaces as the result of a major disruption. During the current decay period of a major thermal disruption, the energy that can damage a wall or limiter comes from the external magnetic field. By providing a good conducting torus near the plasma and increasing the plasma circuit resistance, this magnetic energy (transferred by way of the plasma circuit) can be minimized. This report addresses engineering design features to reduce the energy deposited on the inner torus surface that produces melting of the structures.
The General Assembly (1925)

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The tumour-associated antigen L6 (L6-Ag) is recruited to the tetraspanin-enriched microdomains: implication for tumour cell motility (2008)

Tumour-associated antigen L6 (L6-Ag, also known as TM4SF1) regulates tumour cell motility and invasiveness. We found that L6-Ag is abundant on the plasma membrane and on intracellular vesicles, on which it is co-localised with the markers for late endosomal/lysosomal compartments, including Lamp1/Lamp2 proteins and LBPA. Antibody internalisation and live-imaging experiments suggested that L6-Ag is targeted to late endocytic organelles (LEO) predominantly via a biosynthetic pathway. Mapping experiments showed that the presence of transmembrane regions is sufficient for directing L6-Ag to LEO. On the plasma membrane, L6-Ag is associated with tetraspanin-enriched microdomains (TERM). All three predicted cytoplasmic regions of L6-Ag are crucial for the effective recruitment of the protein to TERM. Recruitment to TERM correlated with the pro-migratory activity of L6-Ag. Depletion of L6-Ag with siRNA has a selective effect on the surface expression of tetraspanins CD63 and CD82. By contrast, the expression levels of other tetraspanins and beta1 integrins was not affected. We found that L6-Ag is ubiquitylated and that ubiquitylation is essential for its function in cell migration. These data suggest that L6-Ag influences cell motility via TERM by regulating the surface presentation and endocytosis of some of their components.
Intracardiac Voltage Gradients during Transthoracic Defibrillation: Implications for Postshock Myocardial Injury (2005)

In-vitro studies indicate that the electric-field intensity, or voltage gradient (VG), generated by a defibrillation shock is a determinant of defibrillation success as well as potential shock-induced cardiac injury. It is not clear how common descriptors of shock dose, e.g., joules (J), relate to VGs. Objectives: To assess the relationships between shock energy descriptors and VG. Methods: One monophasic and three biphasic waveforms were compared using transthoracic shocks and standard electrodes in five swine. VG measurements via intracavitary multielectrode-tipped catheters were compared with delivered energy and peak current. Shock variables were recorded at native transthoracic impedance and an adjusted impedance approximating that of typical humans. Results: For shocks at the same energy setting, peak current and VGs varied widely among the four defibrillators. At simulated human impedance (75 Ω), shocks at each device's maximum energy setting produced similar VGs among the three biphasic defibrillators, despite different delivered energies. For both native impedance (35 Ω) and at 75 Ω, VG correlated with peak current (r = 0.81 and 0.77, respectively) but not energy setting (r = 0.61, 0.52) or delivered energy (r = 0.58, 0.56). At the maximum energy setting of each device, maximum recorded VGs for both monophasic (33 V/cm) and biphasic (24 V/cm) defibrillators were less than those reported to cause myocardial injury (>60 to 80 V/cm). Conclusions: Energy descriptors correlate poorly to actual shock intensities. When compared with reported VG thresholds of myocardial injury, this study suggests that risk of injury from critically strong VGs is low for all of these defibrillators and equivalent among tested biphasic waveforms.
A new classification system for bacterial Rieske non-heme iron aromatic ring-hydroxylating oxygenases (2008)

The new classification system provides the following features. First, the new classification system analyzes RHO enzymes as a whole. RwithSecond, the new classification system is not static but responds dynamically to the growing pool of RHO enzymes. Third, our classification can be applied reliably to the classification of incomplete RHOs. Fourth, the classification has direct applicability to experimental work. Fifth, the system provides new insights into the evolution of RHO systems based on enzyme interaction.
In vitro maturation of human neonatal CD4 T lymphocytes. II. Cytokines present at priming modulate the development of lymphokine production. (1994)

The development of naive CD4 T cells into type 1 or type 2 Th cells has been extensively analyzed in the mouse. Using neonatal CD4 T lymphocytes as a source of human naive cells, we report that these cells may be induced to differentiate into effector cells producing predominantly Th1 or Th2 cytokines. After 3 days of stimulation with anti-CD3 mAb immobilized on CD32 transfected mouse fibroblasts, followed by 3 days of culture in the presence of IL-2, neonatal cells acquire the phenotypic and functional characteristics of effector cells. Primed cells are enriched in CD45R0hi and CD31- cells, and upon stimulation with PMA+ ionomycin they release significant amounts of IL-2, IFN-gamma, IL-4, IL-5, and IL-10. Addition of exogenous cytokines during the period of activation with anti-CD3 markedly alters the profile of cytokine production by primed cells: 1) IL-2 uniformly enhances Th1 and Th2 cytokine production; 2) IL-4 markedly enhances the release of IL-4, IL-5, and IL-10 and suppresses that of IFN-gamma; 3) IFN-gamma strongly inhibits IL-4 and IL-5 production but slightly enhances IFN-gamma release; 4) IFN-alpha markedly inhibits IL-4 and IL-5 production and increases the production of both IFN-gamma and of IL-10; 5) TGF-beta suppresses IL-4 and IL-5 (and to a lesser extent IL-2) production but has inconsistent effect on IL-10 and IFN-gamma production. These effects of exogenous cytokines are not associated with an alteration of CD31 expression on primed cells.
PIDE: physical intrusion detection for personal mobile devices (2015)

Tese de mestrado, Engenharia Informática (Arquitectura, Sistemas e Redes de Computadores), Universidade de Lisboa, Faculdade de Ciências, 2015
Adrenomedullin (2002)

ince 1993, with the discovery of adrenomedullin (AM) by Kitamura and coworkers, 1 we have gained many insights into the biology of this new peptide. 3] We now know that AM circulates in plasma and is present in organs and tissues with increases in AM activity in heart failure, hypertension, and renal dysfunction. ] Indeed, exogenous AM administration induces vasodilation and natriuresis with cAMP elevation, resulting in beneficial cardiorenal response in humans with heart failure. Although AM was originally found by monitoring cAMP elevation in platelets, 1 supporting a key role for cAMP, it has been reported that the nitric oxide (NO)-cGMP pathway also contributes to actions of AM.
Power quality improvement in load commutated inverter-fed synchronous motor drives (2010)

The load commutated inverter (LCI)-fed synchronous motor (SM) drive, also known as commutatorless motor (CLM) drive, is popular in high power applications. Most promising features of these drives include high efficiency, economic operation, flexibility of operation and variable speed in high power range, which led to their applications in blowers, fans, pumps, mill drives for a range of industries as mining, water treatment plants, chemical, paper, textile, cement, rolling mills and petrochemical plants. However, the power quality (PQ) concerns have resulted in a set-back for such applications of these drives. This study investigates various topologies for the mitigation of PQ problems in LCI-fed SM drives using multi-pulse AC–DC converters. A set of hybrid topologies is proposed to feed CLM, which use a multi-winding transformer and diode rectifiers with a chopper cascaded named as ‘rectifier-chopper’. These topologies improve PQ at AC mains, that is, reduce total harmonic distortion in the input current and correct the power factor. It also presents a basis for selection of a suitable AC–DC converter for PQ improvement at the input AC mains of the LCI-fed SM drives.
Ultrasensitive and Highly Selective Detection of Alzheimer’s Disease Biomarker Using Two-Photon Rayleigh Scattering Properties of Gold Nanoparticle (2009)

Alzheimer's disease (AD) is a progressive mental disorder disease, which affects 26.6 million people worldwide and estimated increments can be 100 millions by 2050. Since there is no cure at present, early diagnosis of AD is crucial for the current drug treatments. Driven by the need, here we demonstrate for the first time that monoclonal anti-tau antibody-coated gold nanoparticle based two-photon scattering assay can be used for the detection of Alzheimer's tau protein in the 1 pg/mL level which is about 2 orders of magnitude lower than cutoff values (195 pg/mL) for tau protein in CSF (cerebrospinal fluid). We have shown that when anti-tau antibody-coated gold nanoparticles were mixed with 20 ng/mL of tau protein, two-photon Rayleigh scattering intensity (TPRS) increases by about 16 times. The mechanism of TPRS intensity change has been discussed. Our data demonstrated that our TPRS assay is highly sensitive to tau protein and it can distinguish from BSA, which is one of the most abundant protein components in CSF. Our results demonstrate the potential for a broad application of this type of nanobionanotechnology in practical biomedical applications.
Spin valves with NiO pinning layer (1996)

We have studied both top and bottom spin valves with NiO as an antiferromagnetic pinning layer. The largest bias fields of up to /spl sim/130 Oe were seen for NiFe/Cu/NiFe/NiO top spin valves. Larger bias fields appear to be correlated with partially (200)-textured NiO. The NiFe/Cu/NiFe/NiO samples exhibited GMR values in the 3-4% range. Larger GMR values were seen in NiO/Co/Cu/Co and Co/Cu/Co/NiO spin valves; the largest GMR observed was 11.3% for a bottom spin valve sample of this type.