gebhart/patrepeval huggingface dataset:
In Vitro Studies on Regulation of Osteogenic Activities by Electrical Stimulus on Biodegradable Electroactive Polyelectrolyte Multilayers (2014)

In this study, a novel electroactive tetreaniline-containing degradable polyelectrolyte multilayer film (PEM) coating [(poly(l-glutamic acid)-graft-tetreaniline/poly(l-lysine)-graft-tetreaniline)n, (PGA-g-TA/PLL-g-TA)n] was designed and fabricated by layer-by-layer (LbL) assembly method. Compared with the nongrafted PEMs, the tetreaniline-grafted PEMs showed higher roughness and stiffness in micro/nanoscale structures. The special surface characteristics and the typical electroconductive properties were more beneficial for adhesion, proliferation, and differentiation of preosteoblast MC3T3-E1 cells. Moreover, the enhanced effects were observed on the modulation of MC3T3-E1 cells that differentiated into maturing osteoblasts, when the electroactive PEMs were coupled with electrical stimulus (ES), especially in the early phase of the osteoblast differentiation. The alkaline phosphatase (ALP) activity, calcium deposition, immunofluorescence staining, and RT-qPCR were evaluated on the differentiation of preosteoblast. These data indicate that the comprehensive effects through coupling electroactive scaffolds with electrical stimulus are better to develop bioelectric strategies to control cell functions for bone regeneration.
Evaluation of New Seedling-Rearing and Feritilization for Low-Cost and Labour-Saving on Paddy Rice Culture in the Rice-wheat Double Cropping Area of Gunma Prefecture (2006)

群馬県東部平坦地域を中心とした稲麦二毛作地帯の水稲栽培の新育苗技術と施肥技術による低コスト・省力化を経済性, 省力性両面から評価した. 経済性に関しては10 a当たり経費, 省力性に関しては育苗の技術では取り扱い育苗箱積算重量, 施肥技術では10 a当たりの労働時間によって評価を行った. その結果, 新育苗箱育苗法及び培土減量育苗法は経費, 労働時間がそれぞれ30～36％の低減, 平置き出芽法, 本田全量基肥法は作業時間のみ30～33％の低減, 育苗箱全量基肥法は経費が17％, 労働時間は94％の低減となった. 個々の技術を体系化した「新育苗箱育苗法＋平置き出芽法＋本田全量基肥法」及び「培土量減量育苗法＋平置き出芽法＋育苗箱全量基肥法」の2つの体系を「従来育苗箱育苗法＋標準培土育苗法＋無加温積み重ね出芽法＋本田分肥法」から構成される標準技術と比較したところ, 経費が前者で6％, 後者で30％, 労働時間は共に33％の低減となった. また, 後者による現地試験を2か年間実施した結果, 水稲の生育・収量は標準体系とほぼ同等であり, 収量・品質への影響もなく, 導入可能な技術であると判断した.
Characterisation of zinc-binding domains of peroxisomal RING finger proteins using size exclusion chromatography/inductively coupled plasma-mass spectrometry (2007)

We determined the zinc binding stoichiometry of peroxisomal RING finger proteins by measuring sulfur/metal ratios using inductively coupled plasma-mass spectrometry coupled to size exclusion chromatography, a strategy that provides a fast and quantitative overview on the binding of metals in proteins. As a quality control, liquid chromatography-electrospray ionisation-time of flight-mass spectrometry was used to measure the molar masses of the intact proteins. The RING fingers of Pex2p, Pex10p, and Pex12p showed a stoichiometry of 2.0, 2.1, and 1.2 mol zinc/mol protein, respectively. Thus, Pex2p and Pex10p possess a typical RING domain with two coordinated zinc atoms, whereas that of Pex12p coordinates only a single zinc atom.
Incorporation of<i>N</i>-Acetylneuraminic Acid into<i>Haemophilus somnus</i>Lipooligosaccharide (LOS): Enhancement of Resistance to Serum and Reduction of LOS Antibody Binding (2002)

Haemophilus somnus isolates from cases of thrombotic meningoencephalitis, pneumonia, and other disease sites are capable of undergoing a high rate of phase variation in the oligosaccharide component of their lipooligosaccharides (LOS). In contrast, the LOS of commensal strains isolated from the normal reproductive tract phase vary little or not at all. In addition, the LOS of H. somnus shares conserved epitopes with LOS from Neisseria gonorrhoeae, Haemophilus influenzae, and other species that can incorporate sialic acid into their LOS. We now report that growth of disease isolates of H. somnus with CMP-N-acetylneuraminic acid (CMP-NeuAc) or NeuAc added to the medium resulted in incorporation of NeuAc into the LOS. However, NeuAc was not incorporated into the LOS of commensal isolates and one disease isolate following growth in medium containing CMP-NeuAc or NeuAc. Sialylated LOS was detected by an increase in the molecular size or an increase in the amount of the largest-molecular-size LOS electrophoretic bands, which disappeared following treatment with neuraminidase. Sialylated LOS could also be detected by reactivity with Limax flavus agglutinin lectin, which is specific for sialylated species, by dot blot assay; this reactivity was also reversed by neuraminidase treatment. H. somnus strain 2336 LOS was found to contain some sialic acid when grown in medium lacking CMP-NeuAc or NeuAc, although supplementation enhanced NeuAc incorporation. In contrast strain 738, an LOS phase variant of strain 2336, was less extensively sialylated when the growth medium was supplemented with CMP-NeuAc or NeuAc, as determined by electrophoretic profiles and electrospray mass spectrometry. The sialyltransferase of H. somnus strain 738 was confirmed to preferentially sialylate the Gal(beta)-(1-3)-GlcNAc component of the lacto-N-tetraose structure by capillary electrophoresis assay. Enhanced sialylation of the strain 2336 LOS inhibited the binding of monoclonal antibodies to LOS by enzyme immunoassay and Western blotting. Furthermore, sialylation of the LOS enhanced the resistance of H. somnus to the bactericidal action of antiserum to LOS. Sialylation and increased resistance to killing by normal serum also occurred in a deletion mutant that was deficient in the terminal Gal-GlcNAc disaccharide. LOS sialylation may therefore be an important virulence mechanism to protect H. somnus against the host immune system.
Semblanza de Liselott Diem (2008)

El apellido DIEM posee en las Ciencias del Deporte un reconocido prestigio. Una parte importante de los estudiantes que estudiaron en los anos 70 y 80 tuvieron la ocasion de leer los volumenes que sobre Historia del Deporte, este estudioso aleman escribio. Menos conocida fue su esposa Liselott pero no por ello menos importante. Encarno a la mujer investigadora, a la mujer que se preocupo de manera notable por el estudio del comportamiento y desarrollo motor infantil, asi como del Olimpismo moderno convencida como estaba de las ideas de Pierre de Coubertin, asi como por haber contribuido a fundar el Instituto Carl Diem, institucion creada para el desarrollo del conocimiento en el ambito de las Ciencias del Deporte. Sus estudios fueron numerosos y variados, y en espanol se han conocido a traves de sus libros y estudios diversos y variados. Estos libros se caracterizaron por un lenguaje directo, sin tecnicismos, y desarrollados para mostrar a los profesionales la forma organizada y sistematizada de acometer el desarrollo de la competencia motriz infantil, competencia motriz que debia responder a una expresion natural de las potencialidades infantiles en contextos lo mas naturales posibles, esfuerzos que no siempre fueron reconocidos por sus contemporaneos. (Leer + en PDF )
Application of polyimide resin to semiconductor devices in Japan (1988)

Polyimide resins that have been developed and marketed for semiconductor applications by Japanese companies are reviewed. Test results for devices in which polyimide resins are used as the interlayer insulator, alpha-ray shielding, or buffer coating film are presented. The status of the development of new polyimides for semiconductors is discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
RADIO TO SEND AUTO ON-BOARD DIAGNOSTICS (1995)

On-Board Diagnostic Systems (OBDII) will monitor emissions critical components and activate a malfunction indicator light (MIL) when a failure or drift in calibration is likely to result in emissions that exceed 1.5 times a vehicle's applicable standards. Although the desired response is the timely repair of these malfunctions, under California's current inspection and maintenance requirements, malfunctions may go unaddressed for up to two years. This paper addresses the feasibility of incorporating radio transponders into the OBD system that are capable of transmitting fault codes in order to minimize the time between detection and repair.
Effects of ground plane truncations on the array behaviour of a smart antenna terminal with modular architecture (2005)

Distally based medial hemisoleus muscle flap is reliable coverage option for middle and distal third of tibial defects.
A Percolation Approach to Dielectric Breakdown Statistics (1997)

The stochastic breakdown model proposed by Niemeyer et al. has been improved by taking the time evolution into account in the two-dimensional lattice system. A Markov process has been assumed in the breakdown process and `time' has been defined reflecting the distribution of broken bonds in the cluster. The change of breakdown patterns with time predicts various aspects of breakdown phenomena. We have shown that the final breakdown path does not always form a straight line. We have proposed one of the possible origins for the polarity problem and shown that the polarity difference is understood by the relative position of the damaged layer to the starting point of breakdown.
[Induction of specific immunity against tumor endothelial cells by dendritic cells in vitro]. (2005)

DC loaded with Td-EC antigen can effectively induce specific CTLs against Td-ECs in vitro.
50-nm fully depleted SOI CMOS technology with HfO/sub 2/ gate dielectric and TiN gate (2003)

In this paper, we demonstrate for the first time CMOS thin-film metal gate FDSOI devices using HfO/sub 2/ gate dielectric at the 50-nm physical gate length. Symmetric V/sub T/ is achieved for long-channel nMOS and pMOS devices using midgap TiN single metal gate with undoped channel and high-k dielectric. The devices show excellent performance with a I/sub on/=500 /spl mu/A//spl mu/m and I/sub off/=10 nA//spl mu/m at V/sub DD/=1.2 V for nMOSFET and I/sub on/=212 /spl mu/A//spl mu/m and I/sub off/=44 pA//spl mu/m at V/sub DD/=-1.2 V for pMOSFET, with a CET=30 /spl Aring/ and a gate length of 50 nm. DIBL and SS values as low as 70 mV/V nand 77 mV/dec, respectively, are obtained with a silicon film thickness of 14 nm. Ring oscillators with 15 ps stage delay at V/sub DD/=1.2 V are also realized.
Maturation and Specificity of Plasmodium falciparum Subtilisin-like Protease-1, a Malaria Merozoite Subtilisin-like Serine Protease (2000)

Plasmodium falciparum subtilisin-like protease-1 (PfSUB-1) is a protein belonging to the subtilisin-like superfamily of serine proteases (subtilases). PfSUB-1 undergoes extensive posttranslational proteolytic processing. The primary translation product is converted in the parasite endoplasmic reticulum to p54. This is further processed to p47, which accumulates in secretory organelles within the merozoite. Here, we present a detailed study of this processing. In vitro translated PfSUB-1 showed no capacity to undergo autocatalytic processing. However, parasite extracts contain a protease that cleaves the in vitro translated proprotein between Asp(219) and Asn(220) to form two products of 31 (p31) and 54 kDa; the latter was indistinguishable from authentic p54 and remained complexed with p31 in a noncovalent interaction characteristic of that between a subtilase prodomain and its cognate catalytic domain. Cross-linking studies showed that this complex also exists in the parasite. Expression of PfSUB-1 in recombinant baculovirus also resulted in processing to p54. Mutation of the predicted active site serine abolished processing. Recombinant p54 was secreted in a complex with p31, and could be further converted to p47 in vitro. Conversion required calcium, was an intramolecular autocatalytic process, and involved a second cleavage between Asp(251) and Ala(252). A decapeptide based on sequence flanking Asp(219) was efficiently cleaved by recombinant PfSUB-1. We conclude that PfSUB-1 is a subtilase with an unusual substrate specificity and that it is activated by two autocatalytic processing steps.
Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas. (1990)

Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells.
Calculation of time-dependent Bingham plastic flow using marker FEM (2002)

A simple marker FEM solution algorithm is introduced to calculate transient Bingham plastic flows. An axisymmetric pure radial flow problem and a simple problem of mass conservation are first solved for the purpose of checking the FEM algorithm. A slumping flow of cement -paste cased by its own weight is then calculated as an example of the transient Bingham plastic flow.
La aportación de Enile Jaques-Dalcroze en el campo de la pedagogía musical y el movimiento corporal (1996)

En el siguiente articulo se expone la relacion entre musica y movimiento desde la perspectiva de una corriente pedagogica y pionera, basada en la aportacion de Emile Jacques-Dalcroze. Despues de introducirnos en las raices y las bases teoricas del trabajo de Dalcroze, la autora ejemplifica su aficion en la ensenanza de la musica, su relacion con otras artes y su introduccion en Espana.
Cell Transplants Offer Promise for Stroke Recovery (2003)

Cell transplantation is an experimental approach to restore brain function in neurodegenerative disorders such as Parkinson's and Huntington's disease. Transplantation also represents a possible strategy to repair the brain after a stroke. Various cell types are under investigation in experimental stroke studies. This review discusses the different graft sources and presents preliminary data on the transplantation of neural progenitor cells after stroke in rats. Following transplantation, progenitor cells proliferated and differentiated into all the different brain cell types, including neurons, and they repopulated the ischemic infarct. These results suggest that cell transplantation may serve as a future restorative therapy for stroke and other neurologic disorders such as Parkinson's disease, Alzheimer's disease, trauma, and multiple sclerosis.
Free zone electrophoresis of animal cells: Experiments on cell-cell interactions (1985)

The electrophoretically migrating zones wasa monitored. The absence of fluid flows in the direction of migration permits direct measurement of electrophoretic velocities of any material. Sedimentation is orthogonal to electrokinetic motion and the effects of particle-particle interaction on electrophoretic mobility is studied by free zone electrophoresis. Fixed erythrocytes at high concentrations, mixtures of fixed erythrocytes from different animal species, and mixtures of cultured human cells were studied in low ionic strength buffers. The electrophoretic velocity of fixed erythrocytes was not altered by increasing cell concentration or by the mixing of erythrocytes from different species. When zones containing cultured human glial cells and neuroblastoma cells are permitted to interact during electrophoresis, altered migration patterns occur. It is found that cell-cell interactions depends upon cell type.
Ras‐associated nuclear structural change appears functionally significant and independent of the mitotic signaling pathway (1998)

An altered nuclear morphology has been previously noted in association with Ras activation, but little is known about the structural basis, functional significance, signaling pathway, or reproducibility of any such change. We first tested the reproducibility of Ras-associated nuclear change in a series of rodent fibroblast cell lines. After independently developing criteria for recognizing Ras-associated nuclear change in a Papanicolaou stained test cell line with an inducible H(T24)-Ras oncogene, two cytopathologists blindly and independently assessed 17 other cell lines. If the cell lines showed Ras-associated nuclear change, a rank order of increasing nuclear change was independently scored. Ras-associated nuclear changes were identified in v-Fes, v-Src, v-Mos, v-Raf, and five of five H(T24)-Ras transfectants consisting of a change from a flattened, occasionally undulating nuclear shape to a more rigid spherical shape and a change from a finely textured to a coarse heterochromatic appearance. Absent or minimal changes were scored in six control cell lines. The two cytopathologists' independent morphologic rank orders were similar (P< .0002). The mitogen signaling pathway per se does not appear to transduce the change since no morphologic alterations were identified in cell lines with activations of downstream components of this pathway—MAPKK or c-Myc—and the rank orders did not correlate with markers of mitotic rate (P > .11). The rank order correlated closely with metastatic potential (P < .0014 and P < .0003) but not with histone H1 composition or global nuclease sensitivity. Based on published studies of five of the cell lines, there may be a correlation between increases in certain nuclear matrix proteins and the Ras-associated nuclear change. J. Cell. Biochem. 70:130–140, 1998. © 1998 Wiley-Liss, Inc.
The Second Intracellular Loop of Metabotropic Glutamate Receptor 1 Cooperates with the Other Intracellular Domains to Control Coupling to G-proteins (1996)

Metabotropic glutamate receptors (mGluR) share no sequence homology with any other G-protein-coupled receptors (GPCRs). The characterization of their G-protein coupling domains will therefore help define the general rules for receptor-G-protein interaction. To this end, the intracellular domains of mGluR3 and mGluR1, receptors coupled negatively to adenylyl cyclase and positively to phospholipase C, respectively, were systematically exchanged. The ability of these chimeric receptors to induce Ca2+ signals were examined in Xenopus oocytes and HER 293 cells. The chimeric receptors that still possessed the second intracellular loop (i2) of these proteins were targeted correctly to the plasma membrane. Consistent Ca2+ signals could be recorded only with chimeric mGluR3 receptors that contains i2 and at least one other intracellular domains of mGluR3 have to be replaced by their mGluR1 equivalent to produce optimal coupling to G protein. These observations indicate that i2 of mGluR1 is a critical element in determining the transduction mechanism of this receptor. These results suggest that i2 of mGluRs may play a role similar to i3 of most other GPCRs in the specificity of coupling to the G-proteins. Moreover, as in many other GPCRs, our data revealed cooperation between the different mGluR intracellular domains to control efficient coupling to G-proteins.
Upper Molecular Weight Limit for the Characterization of Polystyrene in Gel Permeation Chromatography (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUpper Molecular Weight Limit for the Characterization of Polystyrene in Gel Permeation ChromatographyE. L. Slagowski, L. J. Fetters, and Donald McIntyreCite this: Macromolecules 1974, 7, 3, 394–396Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 May 1974https://pubs.acs.org/doi/10.1021/ma60039a024https://doi.org/10.1021/ma60039a024research-articleACS PublicationsRequest reuse permissionsArticle Views203Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Amantadine for the agitated head-injury patient (1988)

Traumatic brain injury may be associated with agitated aggressive behaviour and the potential for injury to the patient and staff. We report two cases of recovering brain injury patients with difficult-to-treat destructive behaviour, whose agitation and aggression responded to amantadine. Direct-acting dopamine agonists such as amantadine may be the preferred treatment for patients with behaviour problems in the acute stages of recovery from coma.
Algin/Calcium Gel as a Raw and Cooked Binder in Structured Beef Steaks (1986)

ABSTRACT Structured meat products which could bind raw as well as cooked would be superior to existing structured products. Therefore, the algin/calcium gel system was, studied. Structured beef steaks were made using three levels of sodium alginate and three levels of CaCO 3 . Three additional treatments were included as controls. Treatments were subjectively evaluated for six raw parameters: discoloration, color intensity, alginate pocket area, alginate pocket size, percentage fat and raw bind. Treatments were also subjectively evaluated for four cooked parameters: aroma, flavor, mouthfeel and cooked bind. The algin/calcium gel mechanism can be used to produce structured beef steaks which bind in both the raw and cooked state. Optimum ingredient levels were 0.8–1.2% sodium alginate, 0.144–0.216% CaCO 3 with 500 ppm sodium erythorbate.
Psoriasis − epidemiology and clinical spectrum (2001)

Despite psoriasis being a common skin disease, there are still a number of unanswered questions. One of these is the prevalence of the disease, as there is a lack of specific data, with the majority of studies reporting estimates only. Population based studies are rare and longitudinal observations on changing prevalence rates are lacking. This contrasts with other T-cell mediated autoimmune diseases where the number of those affected is rising. Epidemiological studies revealed that a distinct group of diseases is quite frequently associated with psoriasis, e.g. arthritis, colitis, diabetes and hypertension. In contrast, atopic dermatitis and allergies are less frequently seen compared to normal rates of occurrence. As the psoriatic immune response pattern relates to activated Th-1 cells, psoriasis and atopic dermatitis appear to be mutually exclusive due to the Th-1/Th-2 dichotomy.