gebhart/patrepeval huggingface dataset:
Role of Sterol Superlattice in Free Radical-Induced Sterol Oxidation in Lipid Membranes (2005)

We developed a new fluorescence assay for sterol oxidation and used it to study the relationship between free radical-induced sterol oxidation and membrane sterol lateral organization. This assay used dehydroergosterol (DHE) as both a membrane probe and a membrane component. Sterol oxidation was induced by a free radical generator, AAPH (2,2'-azobis(2-amidinopropane)dihydrochloride). Using this new assay, we found that, in unilamellar vesicles composed of DHE and 1-palmitoyl-2-oleoyl-l-alpha-phosphatidylcholine (POPC), the initial rate of DHE oxidation induced by AAPH changed with membrane sterol content in an alternating manner, exhibiting a local maximum at 20.3, 22.2, 25.0, 32.3, and 40.0 mol % DHE. These mole fractions correspond to the critical sterol mole fractions C(r) predicted for maximal sterol superlattice formation. In three-component bilayers composed of POPC, cholesterol, and DHE (fixed at 1 and 5 mol %), the initial rate of AAPH-induced DHE oxidation exhibited a biphasic change whenever the total sterol mole fraction, irrespective of the DHE content, was near C(r), indicating that the correlation between sterol oxidation and sterol superlattice formation revealed in this study is not an artifact due to the use of the fluorescent cholesterol analogue DHE. The alternating variation of AAPH-induced sterol oxidation with sterol content also appeared in multicomponent unilamellar vesicles containing bovine brain sphingomyelins (bbSPM), POPC, and DHE. The present work and our previous study on cholesterol oxidase-induced sterol oxidation [Wang et al. (2004) Biochemistry 43, 2159-2166] suggest that sterol oxidation in general, either by reactive oxygen species or by enzymes, may be regulated by the extent of sterol superlattice in the membrane and thus regulated by the membrane sterol content in a fine-tuning manner.
Heparin Cofactor II Deficiency (2002)

Consensus was reached that there is insufficient evidence to recommend testing for heparin cofactor II deficiency in patients with thromboembolic disease.
High Efficiency and Robust Performance of Organo Lead Perovskite Solar Cells with Large Grain Absorbers Prepared in Ambient Air Conditions (2014)

Abstract Organo lead halide perovskite absorbers were prepared by a two-step solution-processing method under ambient air conditions. Large cuboid crystalline grains that form a porous surface in junction with an organic hole conductor enable power conversion efficiency up to 15.3%, exhibiting minimal J–V hysteresis and stable performance against light intensity change. The cell without encapsulation showed sufficiently high stability under exposure to air.
Comprehensive isomorphic subtree enumeration (2008)

A fundamental problem in program analysis and optimization concerns the discovery of structural similarities between different sections of a given program and/or across different programs. Specifically, there is a need to find topologically identical segments within compiler intermediate representations (IRs).
Laboratory evaluation of resistance to moisture damage in asphalt mixtures (2012)

Moisture damage in asphalt mixtures refers to loss in strength and durability due to the presence of water. Egypt road network is showing severe deterioration such as raveling and stripping because the bond between aggregates and asphalt film is broken due to water intrusion. To minimize moisture damage, asphalt mixes are investigated to evaluate the effect of air voids, degree of saturation, media of attack and the conditioning period. Two medias of attack are considered and two anti-stripping additives are used (hydrated lime and Portland cement). The retained Marshall stability and tensile strength ratio are calculated to determine the resistance to moisture damage. The results showed that both lime and cement could increase Marshall stability, resilient modulus, tensile strength and resistance to moisture damage of mixtures especially at higher condition periods. Use of hydrated lime had better results than Portland cement.
Phase Slips and Phase Synchronization of Coupled Oscillators (1998)

The behaviors of coupled oscillators, each of which has periodic motion with random natural frequency in the absence of coupling, are investigated. Some novel collective phenomena are revealed. At the onset of instability of the phase-locking state, simultaneous phase slips of all oscillators and quantized phase shifts in these phase slips are observed. By increasing the coupling, a bifurcation tree from high-dimensional quasiperiodicity to chaos to quasiperiodicity and periodicity is found. Different orders of phase synchronizations of chaotic oscillators and chaotic clusters play the key role for constructing this tree structure.
Enzyme-linked immunosorbent assay for chromogranin A. (1989)

This is an enzyme-linked immunosorbent assay (ELISA) for determining chromogranin A (CGA) with use of a monoclonal antibody. CGA was isolated from bovine chromaffin granules. The analytical ELISA procedure for bovine CGA was developed and optimized. Typical standard curves ranged from 500 pg to 500 ng of CGA. We then studied human plasma CGA-immunoreactivity as measured by this assay. The curve for dilutions of human plasma paralleled the standard curve for bovine CGA. The intra-assay coefficient of variation for determination of human plasma CGA was 4.56%, indicating that reliable determinations can be performed for human plasma. However, further study revealed the presence of two CGA-immunoreactive substances in human plasma, one of which corresponds to the native CGA. The nature of the second immunoreactive substance still remains unknown. Nevertheless the measured CGA concentrations (ranging from 0.19 to 0.35 mg/L) in plasma are comparable with previously reported values.
A point dose method for<i>in vivo</i>range verification in proton therapy (2008)

Range uncertainty in proton therapy is a recognized concern. For certain treatment sites, less optimal beam directions are used to avoid the potential risk, but also with reduced benefit. In vivo dosimetry, with implanted or intra-cavity dosimeters, has been widely used for treatment verification in photon/electron therapy. The method cannot, however, verify the beam range for proton treatment, unless we deliver the treatment in a different manner. Specifically, we split the spread-out Bragg peaks in a proton field into two separate fields, each delivering a 'sloped' depth-dose distribution, rather than the usual plateau in a typical proton field. The two fields are 'sloped' in opposite directions so that the total depth-dose distribution retains the constant dose plateau covering the target volume. By measuring the doses received from both fields and calculating the ratio, the water-equivalent path length to the location of the implanted dosimeter can be verified, thus limiting range uncertainty to only the remaining part of the beam path. Production of such subfields has been experimented with a passive scattering beam delivery system. Phantom measurements have been performed to illustrate the application for in vivo beam range verification.
Anoxic LTP is mediated by the redox modulatory site of the NMDA receptor (1994)

1. The effects of redox reagents, 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) and tris(carboxyethyl)phosphine (TCEP), on anoxia-induced long-term potentiation (LTP) were investigated in CA1 hippocampal neurons using extracellular recording techniques. Experiments were performed in the presence of 0.1 mM MgCl2 and 10 microM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) to pharmacologically isolate N-methyl-D-aspartate (NMDA) receptor-mediated responses. 2. DTNB (200 microM), a thiol oxidizing reagent, reduces by 52 +/- 9% (mean +/- SE) (n = 9/9) NMDA-receptor field potentials evoked by electrical stimulation of Schaffer collaterals and this effect could not be reversed by extensive washing. Nearly the same reduction of the initial response was obtained with different concentrations of DTNB (100 and 500 microM), but the time required to reach the maximal inhibition was concentration-dependent. 3. In keeping with an earlier study oxygen and glucose deprivation for 2-3 min induced a long-term potentiation (LTP) of the NMDA receptor response (+65 +/- 16%, n = 4/6). This potentiation was reversed by DTNB (100-500 microM) (-47 +/- 18%; n = 4/4) and the initial LTP could not be restored upon extensive washing of the drug. 4. TCEP (200 microM), a reagent which reduces S-S bond, amplified the electrically evoked NMDA-receptor EPSP (+27 +/- 12%; n = 3). In addition, TCEP (200 microM), nearly completely reversed the effect of DTNB (200 microM) on anoxia-induced LTP (+56 +/- 19%; n = 3/3). Preliminary results also indicate that TCEP occlude anoxic-LTP (n = 3/4). 5. Following DTNB (200 microM) treatment, oxygen and glucose deprivation did not generate anoxic LTP and extensive washing did not restore a potentiated NMDA field potential. 6. These observations strongly suggest that the redox site of the NMDA receptor is involved in the induction and the maintenance of the anoxic LTP of the NMDA receptor-mediated response in CA1.
Parallel coordinate descent methods for big data optimization (2015)

In this work we show that randomized (block) coordinate descent methods can be accelerated by parallelization when applied to the problem of minimizing the sum of a partially separable smooth convex function and a simple separable convex function. The theoretical speedup, as compared to the serial method, and referring to the number of iterations needed to approximately solve the problem with high probability, is a simple expression depending on the number of parallel processors and a natural and easily computable measure of separability of the smooth component of the objective function. In the worst case, when no degree of separability is present, there may be no speedup; in the best case, when the problem is separable, the speedup is equal to the number of processors. Our analysis also works in the mode when the number of blocks being updated at each iteration is random, which allows for modeling situations with busy or unreliable processors. We show that our algorithm is able to solve a LASSO problem involving a matrix with 20 billion nonzeros in 2 h on a large memory node with 24 cores.
Nickel Compounds (2005)

Abstract The article contains sections titled: 1. Introduction 2. Inorganic Nickel Compounds 3. Nickel Tetracarbonyl 4. Nickel Salts of Organic Acids 5. Organonickel Compounds 6. Analysis 7. Economic Aspects 8. Toxicology 8.1. Distribution in the Environment 8.2. Mammalian Toxicology
Efficient Boolean matching in technology mapping with very large cell libraries (2002)

A new method for quickly retrieving Boolean functions from an arbitrarily large library is introduced. The method relies on signature calculation for variables of Boolean functions. Signatures induce an ordering of the variables which is used to construct a BDD (binary decision diagram). The potential of the method is demonstrated by an application in technology mapping for Actel FPGAs (field programmable gate arrays). The proposed approach can handle any kind of gate in the library. Experimental results show that libraries 3 to 4 orders of magnitude larger than those previously used can be dealt with efficiently.
Abstract 4606: Preclinical characterization of a novel fully human IgG1 anti-PD-L1 mAb CK-301 (2017)

Abstract Antibodies targeting Programmed Death-1 (PD-1), or its ligand, PD-L1, have demonstrated remarkable efficacy in subsets of cancer patients, with inhibition of the interaction between PD-1 on T-cells and PD-L1 on tumor cells leading to the recovery of anti-tumor immune response and immune-mediated eradication of tumors. However, not all patients respond to existing PD-1 and PD-L1 targeting agents and relapses to therapy still occur. Therefore, there exists a need to identify additional therapeutics and approaches to engage the immune system to enhance the efficacy of current anticancer therapies. Using phage and yeast display approaches, we have discovered and optimized a novel, fully human, PD-L1 specific IgG1 antibody, CK-301, which exhibits subnanomolar binding affinity for PD-L1. CK-301 blocks binding of PD-L1 to both PD-1 and B7-1 in enzyme-linked immunosorbent assays (ELISA) and cell-based competition assays. Using an assay measuring inhibition of a nuclear factor of activated T-cells (NFAT) reporter caused by PD-L1 binding to PD-1, we demonstrate that CK-301 completely reverses reporter inhibition at concentration of less than 1 µg/ml, IC50 of the dose response curve is 80ng/ml. CK-301 enhances IFN-gamma secretion in allogeneic mixed lymphocyte reaction (MLR) using primary human T-cells and immature dendritic cells. CK-301 can also trigger antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) mediated killing of PD-L1+ cell lines, including lymphoma cells. CK-301 has similar subnanomolar affinity for cynomolgus monkey PD-L1 as for human PD-L1, hence we chose Macaca fascicularis for pre-clinical toxicology and safety pharmacology studies. Single-dose administration of CK-301 to monkeys up to the highest tested dose of 100 mg/kg was shown to be safe and demonstrated linear dose-dependent pharmacokinetic (PK) properties over the dose range from 1 to 100 mg/kg with a half-life of 15 days at 100 mg/kg. A first-in-human Phase 1 study of CK-301 is planned to commence in mid-2017. Citation Format: Leonid Gorelik, George Avgerinos, Yune Kunes, Wayne A. Marasco. Preclinical characterization of a novel fully human IgG1 anti-PD-L1 mAb CK-301 [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 4606. doi:10.1158/1538-7445.AM2017-4606
Control of red spider mites resistant to standard miticides (1960)

California Agriculture - All Issues
Technologies for Detection of Babesia microti: Advances and Challenges (2021)

The biology of intraerythrocytic <i>Babesia</i> parasites presents unique challenges for the diagnosis of human babesiosis. Antibody-based assays are highly sensitive but fail to detect early stage <i>Babesia</i> infections prior to seroconversion (window period) and cannot distinguish between an active infection and a previously resolved infection. On the other hand, nucleic acid-based tests (NAT) may lack the sensitivity to detect window cases when parasite burden is below detection limits and asymptomatic low-grade infections. Recent technological advances have improved the sensitivity, specificity and high throughput of NAT and the antibody-based detection of <i>Babesia</i>. Some of these advances include genomics approaches for the identification of novel high-copy-number targets for NAT and immunodominant antigens for superior antigen and antibody-based assays for <i>Babesia</i>. Future advances would also rely on next generation sequencing and CRISPR technology to improve <i>Babesia</i> detection. This review article will discuss the historical perspective and current status of technologies for the detection of <i>Babesia microti</i>, the most common <i>Babesia</i> species causing human babesiosis in the United States, and their implications for early diagnosis of acute babesiosis, blood safety and surveillance studies to monitor areas of expansion and emergence and spread of <i>Babesia</i> species and their genetic variants in the United States and globally.
miRNAkey: a software for microRNA deep sequencing analysis (2010)

Supplementary data are available at Bioinformatics online.
Latest Developments in Static High Frequency Power Sources for Induction Heating (1970)

This paper describes the basic principles of operation and control of three closely related types of static thyristor inverter circuit, which have been developed specifically for induction heating applications. The circuits described constitute a complete and compatible line of static frequency converters, capable of meeting the needs of virtually all practical induction heating applications, requiring frequencies from 180 Hz up to 10 kHz, at power levels from 100 kW upwards.
Key Technologies for Next Generation Thin Film Silicon Solar Cells. Preparation of Ultra-Large Grain Silicon Thin-Films by Excimer-Laser. (2000)

Advanced excimer-laser-annealing (ELA) technologies of Si thin films on glass have been reviewed. ELA-induced lateral grain growth seems attractive from an application view point since grains more than several microns in length can be grown by single shot of an excimer-laser light pulse. There are two technological ways for enhancing lateral growth kinetics. The one is introduction of non-uniform light intensity on the sample surface. Different values of thermal energy density stored in the molten Si film results in its solidification time delay along the sample surface, which triggers the long lateral grain growth. The other is an application of a non-uniform sample structure, which causes a non-uniform heat removal rate, resulting in the solidification time delay. An underlayer plays also very important role in ELA. By using organic SOG as the underlayer, grains more than 20 microns in length could be grown, and by using porous silica the grain size became more than 0.1 mm. These lateral growth technologies seem applicable not only to thin-film transistors but also to solar cells.
An introduction to cybernetics (1956)

Many workers in the biological sciencesphysiologists, psycholo- gists, sociologistsare interested in cybernetics and would like to apply its methods and techniques to their own speciality. Many have, however, been prevented from taking up the subject by an impression that its use must be preceded by a long study of elec- tronics and advanced pure mathematics; for they have formed the impression that cybernetics and these subjects are inseparable.
Leakage Neutron Spectrum from a Bare Pu<sup>239</sup>Critical Assembly (1960)

The leakage neutron spectrum of a bare Pu239 critical assembly has been measured with nuclear emulsions as both radiator and detector. A comparison with similar measurements on the U235 analogue of this assembly indicates a higher average energy for the Pu239 neutrons.
유아 무용교육이 사회적 능력에 미치는 영향 (2006)

유아기에 있어서 신체적 움직임은 유아의 자신의 활동욕구의 발산이나 외부로부터 지원 및 또래와의 활동을 통하여 이루어진다. 유아기 신체놀이는 유아의 발육발달과 유아기 인격형성 그리고 관계형성에 영향을 미치는 중요한 활동으로 여겨지고 있다. 본 연구의 연구대상은 경기도 영통 중심가의 S유치원으로 사회적·경제적으로 중·상류층 자녀들이 다니고 있고 6세반으로 유아 무용교육 특기적성 참여 유아를 임의로 20명을 선정하여 실험집단을 배정하였고 비교집단은 광주 중심가의 S유치원으로 사회적·경제적으로 중·상류층 자녀들이 다니고 있고 6세반으로 유아 무용교육을 참여하지 않은 유아를 임으로 20명을 선정하였다. 이상의 연구방법과 절차를 통하여 유아 무용교육이 사회적 능력에 미치는 영향을 분석·규명한 결과 다음과 같은 결과를 얻었다. 첫째, 유아 무용교육이 사회적 능력에 미치는 영향에서 무용교육에 참여하는 몰입도는 주도성, 책임성, 개방성, 사교성 등에 영향을 미친 반면 참여 강도와 빈도는 주도성과 책임성에 영향을 미친다. 둘째, 유아의 개인적 특성과 무용교육이 사회적 능력에 미치는 영향에서 유아 무용교육이 개인적 특성 병인에 비하여 사회적 능력에 미치는 영향력이 상대적으로 높다. 따라서 조기의 유아 무용 교육은 사회적 발달에 영향을 미치므로 유아교육기관에서는 유아의 사회적 발달 향상을 위해 무용교육프로그램을 지속적으로 실시하는 것이 유아의 사회적 발달을 증진시키는데 영향을 준다고 본다.
Editorial (1980)

Diseases of the Colon & Rectum: January 1980 - Volume 23 - Issue 1 - p 1 doi: 10.1007/BF02587191
Engineering <i>Lactococcus lactis</i> for Production of Mannitol: High Yields from Food-Grade Strains Deficient in Lactate Dehydrogenase and the Mannitol Transport System (2004)

Mannitol is a sugar polyol claimed to have health-promoting properties. A mannitol-producing strain of Lactococcus lactis was obtained by disruption of two genes of the phosphoenolpyruvate (PEP)-mannitol phosphotransferase system (PTS(Mtl)). Genes mtlA and mtlF were independently deleted by double-crossover recombination in strain L. lactis FI9630 (a food-grade lactate dehydrogenase-deficient strain derived from MG1363), yielding two mutant (Delta ldh Delta mtlA and Delta ldh Delta mtlF) strains. The new strains, FI10091 and FI10089, respectively, do not possess any selection marker and are suitable for use in the food industry. The metabolism of glucose in nongrowing cell suspensions of the mutant strains was characterized by in vivo (13)C-nuclear magnetic resonance. The intermediate metabolite, mannitol-1-phosphate, accumulated intracellularly to high levels (up to 76 mM). Mannitol was a major end product, one-third of glucose being converted to this hexitol. The double mutants, in contrast to the parent strain, were unable to utilize mannitol even after glucose depletion, showing that mannitol was taken up exclusively by PEP-PTS(Mtl). Disruption of this system completely blocked mannitol transport in L. lactis, as intended. In addition to mannitol, approximately equimolar amounts of ethanol, 2,3-butanediol, and lactate were produced. A mixed-acid fermentation (formate, ethanol, and acetate) was also observed during growth under controlled conditions of pH and temperature, but mannitol production was low. The reasons for the alteration in the pattern of end products under nongrowing and growing conditions are discussed, and strategies to improve mannitol production during growth are proposed.
High-Efficiency, Long-Term Cardiac Expression of Foreign Genes in Living Mouse Embryos and Neonates (2000)

We have developed a new strategy to achieve efficient and long-term foreign gene expression in both embryonic and postnatal mouse myocardium via direct intracardiac injection of recombinant adenovirus. The strategy should allow the functional assessment of the expression of dominantly acting exogenous genes, overexpression of wild-type genes, and Cre recombinase-mediated gene ablations at the single-cell level in the context of the intact adult mouse myocardium.
Association of polymorphism of serotonin 2A receptor gene with suicidal ideation in major depressive disorder (2000)

There is evidence indicating that density of 5-HT2A receptors is altered in brain regions of depressed suicide victims and in platelets of suicidal subjects with major depression or schizophrenia. Recent studies have also shown an association between the allele C of 102T/C polymorphism in the 5-HT2A receptor gene and schizophrenia. The present investigation tested the hypothesis that the observed changes in 5-HT2A receptor density in platelets of patients with major depression are a trait rather than state phenomenon and are associated with the 102 C allele in 5-HT2A receptor gene in a sample of 120 patients with major depression and a group of 131 control subjects comparable with respect to age, sex, and ethnic background. The allele and genotype frequencies of 102T/C polymorphism in 5-HT2A receptor gene were compared between patients and control subjects and between suicidal and non-suicidal patient groups. The major finding of this study was a significant association between the 102 C allele in 5-HT2A receptor gene and major depression, chi(2) = 4.5, df = 1, P = 0.03, particularly in patients with suicidal ideation, chi(2) = 8.5, df = 1, P < 0.005. Furthermore, we found that patients with a 102 C/C genotype had a significantly higher mean HAMD item 3 score (indication of suicidal ideation) than T/C or T/T genotype patients. Our results suggest that the 102T/C polymorphism in 5-HT2A receptor gene is primarily associated with suicidal ideation in patients with major depression. Am. J. Med. Genet. (Neuropsychiatr. Genet.) 96:56-60, 2000.
ICRF-187 permits longer treatment with doxorubicin in women with breast cancer. (1992)

By protecting against cumulative doxorubicin-induced cardiac toxicity, ICRF-187 permits significantly greater doses of doxorubicin to be administered to patients with greater safety.