gebhart/patrepeval huggingface dataset:
Allosteric Modulation of β-Arrestin-biased Angiotensin II Type 1 Receptor Signaling by Membrane Stretch (2014)

It has recently been appreciated that the angiotensin II type 1 receptor (AT1R), a prototypic member of the G protein-coupled receptor superfamily, also functions as a mechanosensor. Specifically, mechanical stretch activates the AT1R to promote downstream signaling mediated exclusively by the multifunctional scaffold protein, β-arrestin, in a manner consistent with previously identified β-arrestin-biased ligands. However, the ligand-independent mechanism by which mechanical stretch promotes β-arrestin-biased signaling remains unknown. Implicit in the concept of biased agonism (i.e. the ability of an agonist to activate a subset of receptor-mediated signaling pathways) is the notion that distinct active conformations of the receptor mediate differential activation of signaling pathways. Here we determined whether mechanical stretch stabilizes distinct β-arrestin-activating conformations of the AT1R by using β-arrestin2-biased agonists as conformational probes in pharmacological and biophysical assays. When tested at cells expressing the AT1R fused to β-arrestin (AT1R-β-arrestin2), we found that osmotic stretch increased the binding affinity and potency of the β-arrestin-biased agonist TRV120023, with no effect on the balanced agonist AngII. In addition, the effect of osmotic stretch on ERK activation was markedly augmented in cells expressing the AT1R-β-arrestin2 fusion compared with the wild type AT1R and completely blocked in cells expressing the AT1R-Gq fusion. Biophysical experiments with an intramolecular BRET β-arrestin2 biosensor revealed that osmotic stretch and TRV120023 activate AT1Rs to stabilize β-arrestin2 active conformations that differ from those stabilized by the AT1R activated by angiotensin II. Together, these data support a novel ligand-independent mechanism whereby mechanical stretch allosterically stabilizes specific β-arrestin-biased active conformations of the AT1R and has important implications for understanding pathophysiological AT1R signaling.
Congenital malformations in twins in England and Wales. (1991)

Several major malformations were significantly more common in twins than in singletons. The excess of indeterminate sex and pseudohermaphroditism has not been described before and may be analogous to freemartinism in cattle. Most of the conditions less common in twins are minor, and the reported deficits may be due to underascertainment of the less serious conditions in twins. Down's syndrome is an exception, and the deficit may well be real.
Term indexing for retrieval by unification (2003)

A method is presented for indexing terms in a knowledge-base retrieval-by-unification (RBU) system. The term is a well-defined structure which represents knowledge using variables. RBU operations are an extension of relational database operations using unification and backtracking to retrieve terms from term relations. The term indexing proposed uses hashing and trie structures to reduce the number of comparisons between elements of a search condition and of an object term relation. Unification on a trie structure is suited to backtracking bindings of variables. The search and updating speed of an RBU prototype is measured to evaluate the indexing method. This method is effective for fast term retrieval for a large number of similar and varied form terms. The overhead for maintaining indexes in updating is low.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pathophysiology of Pancreatitis (1999)

Acute pancreatitis is an inflammatory disease, which varies in severity from mild to severe. Factors determining the severity of pancreatitis are not known. It is generally believed that the earliest events in the evolution of acute pancreatitis lead to premature intra-acinar cell activation of digestive zymogens and that those enzymes, once activated cause acinar cell injury. Recent studies have suggested that the ultimate severity of resulting pancreatitis may be determined by events which occur subsequent to acinar cell injury. These include inflammatory cell recruitment and activation as well as the generation and release of cytokines and other chemical mediators of inflammation. Recently, we have undertaken studies to elucidate the role of various inflammatory agents in determining the severity of pancreatitis. Results from these ongoing studies indicate that substance P acting via neurokinin-1 (NK1) receptors, chemokines interacting with CCR1 receptors and platelet activating factor play an important pro-inflammatory role in regulating the severity of pancreatitis and associated lung injury. On the other hand, complement factor 5a (C5a) acts as an anti-inflammatory agent during the development of pancreatitis.
Diethylstilbestrol. A novel F0-directed probe of the mitochondrial proton ATPase. (1986)

At low concentrations, diethylstilbestrol (DES) is shown to be a potent F0-directed inhibitor of the F0F1-ATPase of rat liver mitochondria. In analogy to other F0-directed inhibitors, DES inhibits both the ATPase and ATP-dependent proton-translocation activities of the purified and membrane bound enzyme. When added at low concentrations with dicyclohexylcarbodiimide (DCCD), a covalent inhibitor, DES acts synergistically to inhibit ATPase activity of the complex. At higher concentrations, DES restores DCCD-inhibited ATPase activity. However, there is no restoration of ATP-dependent proton translocation. Under these conditions DCCD remains covalently bound to the F0F1-ATPase complex and F1 remains bound to Fo. Significantly, when the F0F1-ATPase is inhibited by the Fo-directed inhibitor venturicidin rather than DCCD, DES is also able to restore ATPase activity. In contrast, DES is unable to restore ATPase activity to F0F1 preparations inhibited by the Fo-directed inhibitors oligomycin or tricyclohexyltin. However, combinations of [DES + DCCD] or [DES + venturicidin] can restore ATPase activity to F0F1 preparations inhibited by either oligomycin or tricyclohexyltin. Results presented here indicate that the F0 moiety of the rat liver mitochondrial proton ATPase contains a distinct binding site for DES. In addition, they suggest that at saturating concentrations simultaneous occupancy of the DES binding site and sites for either DCCD or venturicidin promote "uncoupled" ATP hydrolysis.
Vallejo entre “dos aguas encontradas” en Los heraldos negros (2019)

Interpretacion de “Los anillos fatigados” de Cesar Vallejo, contenido en Los heraldos negros, como un ejemplo del septimo tipo de ambiguedad de William Empson en el que un texto sugiere simultaneamente dos significados opuestos, expresando asi una division fundamental en la mente del autor. La alusion en el verso 8 al “dedo deicida” del poeta es vista como un eco de la filosofia de Nietzsche. Se plantea que la lucha entre este nuevo estilo poetico —la pose de Zaratustra— y el estilo modernista y rebuscado de Vallejo opera en el corazon de “Los anillos fatigados”, poema que esencialmente se niega a lograr una resolucion semantica.
Neutron Scattering Studies of the Effects of Formulating Amphotericin B with Cholesteryl Sulfate on the Drug’s Interactions with Phospholipid and Phospholipid–Sterol Membranes (2015)

Langmuir surface pressure, small-angle neutron scattering (SANS), and neutron reflectivity (NR) studies have been performed to determine how formulation of the antifungal drug amphotericin B (AmB), with sodium cholesteryl sulfate (SCS)-as in Amphotec-affects its interactions with ergosterol-containing (model fungal cell) and cholesterol-containing (model mammalian cell) membranes. The effects of mixing AmB in 1:1 molar ratio with cholesteryl sulfate (yielding AmB-SCS micelles) are compared against those of free AmB, using monolayers and bilayers formed from palmitoyloleoylphosphatidylcholine (POPC) in the absence and presence of 30 mol % ergosterol or cholesterol, in all cases employing a 1:0.05 molar ratio of lipid:AmB. Analyses of the (bilayer) SANS and (monolayer) NR data indicate that the equilibrium changes in membrane structure induced in sterol-free and sterol-containing membranes are the same for free AmB and AmB-SCS. Stopped-flow SANS experiments, however, reveal that the structural changes to vesicle membranes occur far more rapidly following exposure to AmB-SCS vs free drug, with the kinetics of these changes varying with membrane composition. With POPC vesicles, the structural changes induced by AmB-SCS become apparent only after several minutes, and equilibrium is reached after ∼30 min. The corresponding onset of changes in POPC-ergosterol and POPC-cholesterol vesicles, however, occurs within ∼5 s, with equilibrium reached after 10 and 120 s, respectively. The rate of insertion of AmB into POPC-sterol membranes is thus increased through formulation as AmB-SCS. Moreover, the differences in monolayer surface pressure and SANS structure-change equilibration times suggest significant rearrangement of AmB within these membranes following insertion. The reduced times to equilibrium for the POPC-ergosterol vs POPC-cholesterol systems are consistent with the known differences in affinity of AmB for these two sterols, and the reduced time to equilibrium for AmB-SCS interaction with POPC-ergosterol membranes vs that for free AmB is consistent with the reduced host toxicity of Amphotec.
Bis(alkylguanidinium) receptors for phosphodiesters: effect of counterions, solvent mixtures, and cavity flexibility on complexation (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBis(alkylguanidinium) receptors for phosphodiesters: effect of counterions, solvent mixtures, and cavity flexibility on complexationDiane M. Kneeland, Katsuhiko Ariga, Vincent M. Lynch, Chia Yu Huang, and Eric V. AnslynCite this: J. Am. Chem. Soc. 1993, 115, 22, 10042–10055Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/ja00075a021https://doi.org/10.1021/ja00075a021research-articleACS PublicationsRequest reuse permissionsArticle Views2032Altmetric-Citations157LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (4)»Supporting Information Supporting Information Get e-Alerts
Water Uptake and Hygroscopic Growth of Organosulfate Aerosol (2016)

Organosulfates (OS) are important components of secondary organic aerosol (SOA) that have been identified in numerous field studies. This class of compounds within SOA can potentially affect aerosol physicochemical properties such as hygroscopicity because of their polar and hydrophilic nature as well as their low volatility. Currently, there is a dearth of information on how aerosol particles that contain OS interact with water vapor in the atmosphere. Herein we report a laboratory investigation on the hygroscopic properties of a structurally diverse set of OS salts at varying relative humidity (RH) using a Hygroscopicity-Tandem Differential Mobility Analyzer (H-TDMA). The OS studied include the potassium salts of glycolic acid sulfate, hydroxyacetone sulfate, 4-hydroxy-2,3-epoxybutane sulfate, and 2-butenediol sulfate and the sodium salts of benzyl sulfate, methyl sulfate, ethyl sulfate, and propyl sulfate. In addition, mixtures of OS and sodium chloride were also studied. The results showed gradual deliquescence of these aerosol particles characterized by continuous uptake and evaporation of water in both hydration and dehydration processes for the OS, while the mixture showed prompt deliquescence and effloresce transitions, albeit at a lower relative humidity relative to pure sodium chloride. Hygroscopic growth of these OS at 85% RH were also fit to parameterized functional forms. This new information provided here has important implications about the atmospheric lifetime, light scattering properties, and the role of OS in cloud formation. Moreover, results of these studies can ultimately serve as a basis for the development and evaluation of thermodynamic models for these compounds in order to consider their impact on the atmosphere.
Isolation and characterization of an atrazine-degrading bacterium from industrial wastewater in China (2003)

Comparison analysis of atrazine degradation gene structure and arrangement in this and other bacteria provides insight into our understanding of the ecology and evolution of atrazine-degrading bacteria.
Use of Propidium Monoazide for Live/Dead Distinction in Microbial Ecology (2007)

One of the prerequisites of making ecological conclusions derived from genetic fingerprints is that bacterial community profiles reflect the live portion of the sample of interest. Propidium monoazide is a membrane-impermeant dye that selectively penetrates cells with compromised membranes, which can be considered dead. Once inside the cells, PMA intercalates into the DNA and can be covalently cross-linked to it, which strongly inhibits PCR amplification. By using PCR after PMA treatment, the analysis of bacterial communities can theoretically be limited to cells with intact cell membranes. Four experiments were performed to study the usefulness of PMA treatment of mixed bacterial communities comprising both intact and compromised cells in combination with end-point PCR by generating community profiles from the following samples: (i) defined mixtures of live and isopropanol-killed cells from pure cultures of random environmental isolates, (ii) wastewater treatment plant influent spiked with defined ratios of live and dead cells, (iii) selected environmental communities, and (iv) a water sediment sample exposed to increasing heat stress. Regions of 16S rRNA genes were PCR amplified from extracted genomic DNA, and PCR products were analyzed by using denaturing gradient gel electrophoresis (DGGE). Results from the first two experiments show that PMA treatment can be of value with end-point PCR by suppressing amplification of DNA from killed cells. The last two experiments suggest that PMA treatment can affect banding patterns in DGGE community profiles and their intensities, although the intrinsic limitations of end-point PCR have to be taken into consideration.
High-Sensitivity Force Detection by Phase-Modulation Atomic Force Microscopy (2006)

Performance of phase-modulation atomic force microscopy (PM-AFM) is investigated to achieve high force sensitivity and high-resolution imaging. PM-AFM detects the phase change of the oscillating cantilever relative to the excitation signal and uses it as the feedback signal. We compare the force sensitivity of PM-AFM with that of amplitude-modulation AFM (AM-AFM) theoretically as well as experimentally. We show that PM-AFM has a better signal-to-noise ratio than AM-AFM. We demonstrate that the electrostatic force images obtained by PM-AFM have clearer contrast than those obtained by AM-AFM.
Insulin-like Effect of Zinc on Adipocytes (1980)

In view of the known effect of zinc on the crystalline nature of insulin, we have investigated whether zinc alters the biologic potency of this hormone. Using the rate of lipogenesis by rat epididymal adipocytes as an index of the biologic potency of insulin, we have shown that zinc exerts a potent stimulatory effect upon lipogenesis in vitro similar to, but quite independently of, insulin and that it has an additive effect with that of insulin when both are incubated together. This effect of zinc on adipocytes and the biologic potency of insulin, hitherto unreported, is of significance at the biologic, pharmacologic, and clinical level.
Web-based monitoring of year-length deployments of autonomous gas sensing platforms on landfill sites (2011)

This paper describes multiple field deployments of autonomous gas monitoring platforms spanning durations in excess of 12 months. These trials form part of an on-going collaboration with the Environmental Protection Agency (EPA) in monitoring landfill migration of greenhouse gases, i.e. methane (CH <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> ) and carbon dioxide (CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ). Target gas concentrations were automatically recorded via infrared (IR) gas sensors calibrated for the respective gases, with this data being logged remotely every six hours to a central base-station. The autonomous platform with its web-based portal interface provides a flexible alternative to the existing labor-intensive, manual monitoring routines. The duration of the data herein represents one of the longest continuous field deployments of an environmental monitoring device. The real-time monitoring of the gas levels achieved by the bespoke platforms has proven to contribute significantly towards the improved management of landfill sites.
Two new saponins from Anemarrhena asphodeloides Bge. (2007)

Two new saponins 3-O-β-D-glucopyranosyl (1→2)-β-D-mannopyranosyl sarsasapogenin, named timosaponin A IV(1) and (5β, 25S)-26-O-β-D-glucopyranosyl-furost-20(22)-en-3,26-diol-3-O-β-D-glucopyranosyl (1→4) glucopyranosyl (1→2)-β-D-galacopyranoside, named timosaponin B IV(2), were isolated by silica gel column chromatography and preparative HPLC from Anemarrhena asphodeloides Bge. Their structures were elucidated by chemical characters and spectroscopic analysis.
Graphite as Cointercalation Electrode for Sodium‐Ion Batteries: Electrode Dynamics and the Missing Solid Electrolyte Interphase (SEI) (2018)

Abstract The intercalation of solvated sodium ions into graphite from ether electrolytes was recently discovered to be a surprisingly reversible process. The mechanisms of this “cointercalation reaction” are poorly understood and commonly accepted design criteria for graphite intercalation electrodes do not seem to apply. The excellent reversibility despite the large volume expansion, the small polarization and the puzzling role of the solid electrolyte interphase (SEI) are particularly striking. Here, in situ electrochemical dilatometry, online electrochemical mass spectrometry (OEMS), a variety of other methods among scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X‐ray diffraction (XRD) as well as theory to advance the understanding of this peculiar electrode reaction are used. The electrode periodically “breathes” by about 70–100% during cycling yet excellent reversibility is maintained. This is because the graphite particles exfoliate to crystalline platelets but do not delaminate. The speed at which the electrode breathes strongly depends on the state of discharge/charge. Below 0.5 V versus Na + /Na, the reaction behaves more pseudocapacitive than Faradaic. Despite the large volume changes, OEMS gas analysis shows that electrolyte decomposition is largely restricted to the first cycle only. Combined with TEM analysis and the electrochemical results, this suggests that the reaction is likely the first example of a SEI‐free graphite anode.
Deposition of CuInAlSe<inf>2</inf> films using co-sputtered precursors and selenization (2009)

CuInAl precursor films with varying Al/(Al+In) ratios were co-sputtered onto Mo coated soda-lime glass substrates. Samples were then selenized under high vacuum using thermally evaporated elemental selenium. Single-step and two-step selenization heating profiles were investigated. Single-step samples resulted in better Mo-CuInAlSe <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> adhesion than those selenized using the two-step approach. Precursor and selenized films were characterized for composition, crystalline structure, surface morphology, and composition with depth. The development of a method to co-sputtered CuInAl precursors is discussed, as well as the effect of selenization temperature and heating profile on selenized film properties.
Photochemical isomerisation derivatives of decafluorocyclohexene and their reactions (1971)

Perfluoro-(methylenecyclopentane) and -(1-methylcyclopentene), together with some higher-boiling compounds, have been obtained by the u.v.-irradiation of decafluorocyclohexene. Perfluoro-(methylenecyclopentane) underwent addition of chlorine and isomerisation to perfluoro-(1-methylcyclopentene), catalysed by fluoride ion; on treatment with sodium borohydride in bis-(2-methoxyethyl) ether it gave 1H-hexafluoro-2-methylcyclopentene, 3,3,4,4-tetrafluoro-1-trifluoromethylcyclopentene, and five polyfluoro-olefins as minor components. Both perfluoro-(methylenecyclopentane) and -(1-methylcyclopentene), on treatment with sodium iodide in acetone, afforded hexafluoro-1-iodo-2-trifluoromethylcyclopentene, which reacted with magnesium in ether to give a Grignard reagent, and with pentafluoroiodobenzene and copper bronze to give perfluoro-(2,2′-dimethylbicyclopent-1,1′-enyl) and hexafluoro-1-pentafluorophenyl-2-trifluoromethylcyclopentene. Perfluoro-(methylenecyclopentane) reacted with aluminium chloride in methyl iodide to give 1-(chlorodifluoromethyl)heptafluorocyclopentene, which, on treatment with sodium iodide in acetone, afforded 1-(difluoroiodomethyl)heptafluorocyclopentene. Both compounds could be dehalogenofluorinated to give perfluoro-(3-methylenecyclopentene), which added chlorine and underwent isomerisation, catalysed by fluoride ion, to perfluoro-1- and -2-methylcyclopentadiene. When heated, the former compound gave perfluoro-(1,4-dimethyltricyclo[5,2,1,02,6]deca-3,8-diene), a Diels–Alder dimer, and the latter gave a mixture of dimeric products. From the high-boiling products of the irradiation of decafluorocyclohexene, perfluoro-bicyclohex-1,1′-enyl, -1-cyclohexylcyclohexene, -1,1′-bicyclohexylidene, -1-(cyclohex-1-enyl)-1-methylcyclopentane, -bicyclohexyl, and -1-cyclohexyl-1-methylcyclopentane, were obtained.
αβ Chimeric Antisense Oligonucleotides: Synthesis and Nuclease Resistance in Biological Media (1994)

A new type of chimeric oligonucleotides composed of alpha- and beta-sections is described. The sequence of eight beta-nucleotides flanked at 3'- or/and 5'-ends by nuclease-resistant alpha-oligonucleotides has been chosen as an effector domain to form a substrate for RNase H. The synthesized oligonucleotides are complementary to the translation initiation site of the pim protooncogene mRNA. We used the chemical ligation method to prepare the chimeric oligonucleotides. The thermal stability of heteroduplexes formed by the alpha beta oligonucleotides with a complementary strand is not significantly altered compared to that of their beta-analogs. These oligonucleotides promote efficient RNase H-mediated cleavage of pim mRNA. Among the alpha beta oligonucleotides studied, one with an alpha-fragment bound by its 3'-end to the 3'-end of the beta-octanucleotide proved to be the most resistant to nucleolytic digestion in human plasma, calf serum, and murine fibroblast lysate. This alpha beta oligonucleotide directs more specific RNA cleavage by RNase H than its beta beta counterpart.