gebhart/patrepeval huggingface dataset:
A peripherally restricted cannabinoid receptor agonist produces robust anti-nociceptive effects in rodent models of inflammatory and neuropathic pain (2010)

Cannabinoids are analgesic in man, but their use is limited by their psychoactive properties. One way to avoid cannabinoid receptor subtype 1 (CB1R)-mediated central side-effects is to develop CB1R agonists with limited CNS penetration. Activation of peripheral CB1Rs has been proposed to be analgesic, but the relative contribution of peripheral CB1Rs to the analgesic effects of systemic cannabinoids remains unclear. Here we addressed this by exploring the analgesic properties and site of action of AZ11713908, a peripherally restricted CB1R agonist, in rodent pain models. Systemic administration of AZ11713908 produced robust efficacy in rat pain models, comparable to that produced by WIN 55, 212-2, a CNS-penetrant, mixed CB1R and CB2R agonist, but AZ11713908 generated fewer CNS side-effects than WIN 55, 212-in a rat Irwin test. Since AZ11713908 is also a CB2R inverse agonist in rat and a partial CB2R agonist in mouse, we tested the specificity of the effects in CB1R and CB2R knock-out (KO) mice. Analgesic effects produced by AZ11713908 in wild-type mice with Freund's complete adjuvant-induced inflammation of the tail were completely absent in CB1R KO mice, but fully preserved in CB2R KO mice. An in vivo electrophysiological assay showed that the major site of action of AZ11713908 was peripheral. Similarly, intraplantar AZ11713908 was also sufficient to induce robust analgesia. These results demonstrate that systemic administration of AZ11713908, produced robust analgesia in rodent pain models via peripheral CB1R. Peripherally restricted CB1R agonists provide an interesting novel approach to analgesic therapy for chronic pain.
Plant Regeneration through Anther Culture and Induced Parthenogenesis in Genus<i>Helianthus</i> (1997)

Two possibilities, based on the androgenic and gynogenic approach for development of haploid plants in vitro, were studied in the Genus Helianthus. Six F1 interspecific hybrids between the wild species H.decapetalus, H.hirsutus, H.rigidus, H.mollis, H.eggertii and H.laevigatus, and the cultivated H. animus L. have been used as donor plants for anther culture. Anthers were plated on two different callus induction media: AA medium (Alissa et al., 1985) and mod. L4M medium (Lenee P. and Chupea Y., 1985). Depending on the genotype callus induction occurred in a range of 50–100% on the mod. L4M medium and 15–60% on the AA medium. Plant regeneration and shoot multiplication were observed in the F1 hybrids of H.eggertii, H.mollis, H.decapetalus and H.rigidus after the third transfer of the calluses on the B5 hormone free medium (Chandler and Beard, 1983). For inducing gynogenic development 8 wild sunflower species (H. decapetalus, H. hirsutus, H.rigidus, H. eggertii, H. laevigatas, H. ciliaris, H. x laetiflorus and H. strumosus), were pollinated with inactivated through gamma—irradiation pollen of Rf line 147. The formed embryos were cultured in vitro. Seven plants of the variant H.x laetiflorus x Rf 147, where the pollen were treated with 600 Gy gamma rays, were grown in greenhouse.
Selection of Aptamers for Amyloid &beta;-Protein, the Causative Agent of Alzheimer's Disease (2010)

Alzheimer's disease (AD) is a progressive, age-dependent, neurodegenerative disorder with an insidious course that renders its presymptomatic diagnosis difficult(1). Definite AD diagnosis is achieved only postmortem, thus establishing presymptomatic, early diagnosis of AD is crucial for developing and administering effective therapies(2,3). Amyloid beta-protein (Abeta) is central to AD pathogenesis. Soluble, oligomeric Abeta assemblies are believed to affect neurotoxicity underlying synaptic dysfunction and neuron loss in AD(4,5). Various forms of soluble Abeta assemblies have been described, however, their interrelationships and relevance to AD etiology and pathogenesis are complex and not well understood(6). Specific molecular recognition tools may unravel the relationships amongst Abeta assemblies and facilitate detection and characterization of these assemblies early in the disease course before symptoms emerge. Molecular recognition commonly relies on antibodies. However, an alternative class of molecular recognition tools, aptamers, offers important advantages relative to antibodies(7,8). Aptamers are oligonucleotides generated by in-vitro selection: systematic evolution of ligands by exponential enrichment (SELEX)(9,10). SELEX is an iterative process that, similar to Darwinian evolution, allows selection, amplification, enrichment, and perpetuation of a property, e.g., avid, specific, ligand binding (aptamers) or catalytic activity (ribozymes and DNAzymes). Despite emergence of aptamers as tools in modern biotechnology and medicine(11), they have been underutilized in the amyloid field. Few RNA or ssDNA aptamers have been selected against various forms of prion proteins (PrP)(12-16). An RNA aptamer generated against recombinant bovine PrP was shown to recognize bovine PrP-beta(17), a soluble, oligomeric, beta-sheet-rich conformational variant of full-length PrP that forms amyloid fibrils(18). Aptamers generated using monomeric and several forms of fibrillar beta(2;)-microglobulin (beta(2;)m) were found to bind fibrils of certain other amyloidogenic proteins besides beta(2;)m fibrils(19). Ylera et al. described RNA aptamers selected against immobilized monomeric Abeta40(20). Unexpectedly, these aptamers bound fibrillar Abeta40. Altogether, these data raise several important questions. Why did aptamers selected against monomeric proteins recognize their polymeric forms? Could aptamers against monomeric and/or oligomeric forms of amyloidogenic proteins be obtained? To address these questions, we attempted to select aptamers for covalently-stabilized oligomeric Abeta40(21) generated using photo-induced cross-linking of unmodified proteins (PICUP)(22,23). Similar to previous findings(17,19,20), these aptamers reacted with fibrils of Abeta and several other amyloidogenic proteins likely recognizing a potentially common amyloid structural aptatope(21). Here, we present the SELEX methodology used in production of these aptamers(21).
<title>Blind equalization</title> (1991)

Blind equalization deals with the restoration of the input sequence given the output of a linear communication channel and statistical information about the input. A solution to this problem can be achieved by using nonlinear transformations or higher-order statistics of the output sequence. The purpose of this paper is to provide a review of existing adaptive blind equalization algorithms and point out their strengths and limitations.
Chromatin-Remodeling and Memory Factors. New Regulators of Plant Development (2002)

The establishment and heritable maintenance of specific epigenetic states that lead to differential gene expression are crucial for cell differentiation and development. Over the past few years, it has become apparent that epigenetic control of transcription is mediated through specific states of
A millimeter-wave radial line slot antenna fed by a rectangular waveguide through a ring slot (2002)

A radial line slot antenna (RLSA) is a high gain and high efficiency planar antenna proposed for DBS subscribers in the 12 GHz band. In recent years, several radio systems have been developed in the millimeter wave band. In conventional RLS, a radial waveguide has been fed by a coaxial feeder. In the millimeter wave band, fabrication of the feeder is considerably difficult, since the physical size is small. High accuracy in manufacturing is needed to give high antenna efficiency. We have proposed a planar feed circuit with a ring slot and a microstrip line (see Kigure, Y. et al., IEICE Natl. Conv. Rec., B-1-102, 1999). The feed circuit may be easily integrated with an RF planar circuit. In this structure, however, undesired radiation to the back of the antenna is significant and the antenna efficiency is degraded. In terms of efficiency in the millimeter wave band, a rectangular waveguide is more attractive for feeding a radial waveguide. A rectangular-to-radial waveguide transformer through a ring slot is proposed. This transformer is slightly bulky, however there is no leakage. It is suitable for integrating with existing RF modules that have an interface to the rectangular waveguide. The rectangular-to-radial waveguide transformer through a ring slot is designed and fabricated at the 12 GHz band and we integrate a RLSA with this feed circuit at the 38 GHz band.
Conductivity in semiconductors (1949)

This article is a summary of the material on the nature of the conductivity in semiconductors presented at the AIEE Summer General Meeting in a Symposium on Electrical Properties of Semiconductors and the Transistor. Previous articles in the series covered rectification and photoeffects in semiconductors.
Fabrication of GaInP/GaAs//Si Solar Cells by Surface Activated Direct Wafer Bonding (2013)

GaInP/GaAs//Si solar cells with three active p-n junctions were fabricated by surface activated direct wafer bonding between GaAs and Si. The direct wafer bond is performed at room temperature and leads to a conductive and transparent interface. This allows the fabrication of high-efficiency monolithic tandem solar cells with active junctions in both Si and the III-V materials. This technology overcomes earlier challenges of III-V and Si integration caused by the large difference in lattice constant and thermal expansion. Transmission electron microscopy revealed a 5-nm thin amorphous interface layer formed by the argon fast atom beam treatment before bonding. No further defects or voids are detected in the photoactive layers. First triple-junction solar cell devices on Si reached an efficiency of 23.6% under concentrated illumination.
Integrating microsystems with metamaterials towards metadevices (2019)

Electromagnetic metamaterials, which are a major type of artificially engineered materials, have boosted the development of optical and photonic devices due to their unprecedented and controllable effective properties, including electric permittivity and magnetic permeability. Metamaterials consist of arrays of subwavelength unit cells, which are also known as meta-atoms. Importantly, the effective properties of metamaterials are mainly determined by the geometry of the constituting subwavelength unit cells rather than their chemical composition, enabling versatile designs of their electromagnetic properties. Recent research has mainly focused on reconfigurable, tunable, and nonlinear metamaterials towards the development of metamaterial devices, namely, metadevices, via integrating actuation mechanisms and quantum materials with meta-atoms. Microelectromechanical systems (MEMS), or microsystems, provide powerful platforms for the manipulation of the effective properties of metamaterials and the integration of abundant functions with metamaterials. In this review, we will introduce the fundamentals of metamaterials, approaches to integrate MEMS with metamaterials, functional metadevices from the synergy, and outlooks for metamaterial-enabled photonic devices.
The Delphi Method: An Experimental Study of Group Opinion (1969)

This report describes the results of an extensive set of experiments conducted at RAND during the spring and summer of 1968 that were concerned with evaluating the effectiveness of the Delphi procedures for formulating group judgments.
Cytolytic T Lymphocytes Raised Against a Human Bladder Carcinoma Recognize an Antigen Encoded by Gene <i>MAGE-A12</i> (2000)

Human bladder carcinoma line LB831-BLC expresses several distinct Ags that are recognized by different autologous CTL. Here, we show that one of these Ags is presented by HLA-Cw7 and encoded by gene MAGE-A12. This is the first time that CTL directed against a MAGE-encoded Ag have been derived from the lymphocytes of a patient with cancer other than melanoma. This new Ag was found to be nonapeptide VRIGHLYIL, corresponding to position 170-178 of the MAGE-A12 protein. Gene MAGE-A12 is silent in normal tissues except in male germline cells, which do not express HLA molecules. It is expressed in 26-62% of melanomas, infiltrating bladder carcinomas, lung carcinomas, esophageal carcinomas, and head and neck carcinomas. Because HLA-Cw7 is present in 43% of Caucasians, this new Ag is shared by many tumors and should be a useful target for cancer immunotherapy.
A model for the germ tube formation and mycelial growth form of<i>Candida albicans</i> (1984)

A model based on morphological and ultrastructural evidence is presented which illustrates a novel and hitherto undescribed pattern of germ tube formation and hyphal growth in early and mature colonies of Candida albicans. Accordingly, most of the cytoplasm within the parent yeast cell migrates into and forward with the extending germ tubes and leaves behind an extensively vacuolated yeast cell. Growing hyphae similarly are subtended by migrating "slugs' of protoplasm and leave behind vacuolated intercalary compartments. The vacuolated cell compartments apparently must first regenerate their protoplasmic contents before producing branches or secondary germ tubes. This model is used to explain certain unusual features of the growth kinetics of the filamentous form of this organism.
High performance steam development. Final report, Phase No. 3: 1500{degree}F steam plant for industrial cogeneration prototype development tests (1996)

As a key part of DOE`s and industry`s R&D efforts to improve the efficiency, cost, and emissions of power generation, a prototype High Performance Steam System (HPSS) has been designed, built, and demonstrated. The world`s highest temperature ASME Section I coded power plant successfully completed over 100 hours of development tests at 1500{degrees}F and 1500 psig on a 56,000 pound per hour steam generator, control valve and topping turbine at an output power of 5500 hp. This development advances the HPSS to 400{degrees}F higher steam temperature than the current best technology being installed around the world. Higher cycle temperatures produce higher conversion efficiencies and since steam is used to produce the large majority of the world`s power, the authors expect HPSS developments will have a major impact on electric power production and cogeneration in the twenty-first century. Coal fueled steam plants now produce the majority of the United States electric power. Cogeneration and reduced costs and availability of natural gas have now made gas turbines using Heat Recovery Steam Generators (HRSG`s) and combined cycles for cogeneration and power generation the lowest cost producer of electric power in the United States. These gas fueled combined cycles also have major benefits in reducing emissions while reducing the cost of electricity. Development of HPSS technology can significantly improve the efficiency of cogeneration, steam plants, and combined cycles. Figure 2 is a TS diagram that shows the HPSS has twice the energy available from each pound of steam when expanding from 1500{degrees}F and 1500 psia to 165 psia (150 psig, a common cogeneration process steam pressure). This report describes the prototype component and system design, and results of the 100-hour laboratory tests. The next phase of the program consists of building up the steam turbine into a generator set, and installing the power plant at an industrial site for extended operation.
RNA B is the major nucleolar trimethylguanosine-capped small nuclear RNA associated with fibrillarin and pre-rRNAs in Trypanosoma brucei. (1993)

RNA B is one of three abundant trimethylguanosine-capped U small nuclear RNAs (snRNAs) of Trypanosoma brucei which is not strongly identified with other U snRNAs by sequence homology. We show here that RNA B is a highly diverged U3 snRNA homolog likely involved in pre-rRNA processing. Sequence identity between RNA B and U3 snRNAs is limited; only two of four boxes of homology conserved between U3 snRNAs are obvious in RNA B. These are the box A homology, specific for U3 snRNAs, and the box C homology, common to nucleolar snRNAs and required for association with the nucleolar protein, fibrillarin. A 35-kDa T. brucei fibrillarin homolog was identified by using an anti-Physarum fibrillarin monoclonal antibody. RNA B and fibrillarin were localized in nucleolar fractions of the nucleus which contained pre-rRNAs and did not contain nucleoplasmic snRNAs. Fibrillarin and RNA B were precipitated by scleroderma patient serum S4, which reacts with fibrillarins from diverse organisms; RNA B was the only trimethylguanosine-capped RNA precipitated. Furthermore, RNA B sedimented with pre-rRNAs in nondenaturing sucrose gradients, similarly to U3 and other nucleolar snRNAs, suggesting that RNA B is hydrogen bonded to rRNA intermediates and might be involved in their processing.
Subhertz linewidth diode lasers by stabilization to vibrationally and thermally compensated ultralow-expansion glass Fabry-Pérot cavities (2008)

We achieved a 0.5 Hz optical beat note linewidth with $\ensuremath{\sim}0.1\text{ }\text{Hz}/\text{s}$ frequency drift at 972 nm between two external cavity diode lasers independently stabilized to two vertically mounted Fabry-P\'erot (FP) reference cavities with a finesse of 400 000. Vertical FP reference cavities are suspended in midplane such that the influence of vertical vibrations to the mirror separation is significantly suppressed. This makes the setup virtually immune for vertical vibrations that are more difficult to isolate than horizontal vibrations. To compensate for thermal drifts the FP spacers are made from ultralow-expansion (ULE) glass which possesses a zero linear expansion coefficient. A design using Peltier elements in vacuum allows operation at an optimal temperature where the quadratic temperature expansion of ULE could be eliminated as well. The measured linear drift of such ULE FP cavity of 63 mHz/s was due to material aging and the residual frequency fluctuations were less than $\ifmmode\pm\else\textpm\fi{}20\text{ }\text{Hz}$ during 16 h of measurement. Some part of the temperature-caused drift is attributed to the thermal expansion of the mirror coatings. Thermally induced fluctuations that cause vibrations of the mirror surfaces limit the stability of our cavity. By comparing two similar laser systems we obtain an Allan instability of $2\ifmmode\times\else\texttimes\fi{}{10}^{\ensuremath{-}15}$ between 0.1 and 10 s averaging time, which is close to the theoretical thermal noise limit.
Constitutive expression of mhc and adhesion molecules by alveolar epithelial cells (type ii pneumocytes) isolated from human lung and comparison with immunocytochemical findings (1994)

Highly purified populations of alveolar epithelial cells (type II pneumocytes) were isolated from human lung specimens. These cells were characterised histochemically, by demonstrating the presence of intracellular alkaline phosphatase, and morphologically, by electron microscopic demonstration of lamellar bodies and microvilli. Expression of the epithelial glycoprotein HEA-125, of MHC class I and class II (HLA-DR, -DP and -DQ) antigens and of the intercellular adhesion molecules ICAM-1, VCAM-1, LFA-3 and B7 was quantified by flow cytometry. Comparison was made between the expression of these molecules by isolated type II cells and by alveolar epithelium in normal human lung tissue after immunocytochemical staining of frozen sections of donor lung. Isolated type II pneumocytes expressed HEA-125 and class I MHC molecules and the class II MHC molecules HLA-DR and -DP; HLA-DQ was not detected. The intercellular adhesion molecule ICAM-1 was expressed constitutively at low levels but there was minimal expression of VCAM-1, LFA-3 and B7. It was not possible to differentiate type II cells from the predominant type I pneumocytes on frozen sections. Alveolar epithelium expressed HEA-125, class I MHC antigens, the class II molecules HLA-DR, and -DP and the intercellular adhesion molecule LFA-3. Expression of the adhesion molecules ICAM-1, VCAM-1 and B7 was variable. As with the isolates, HLA-DQ was not observed on alveolar epithelium. In conclusion, a reproducible method for the isolation of pure populations of human type II pneumocytes has been developed. These cells were not damaged by the isolation procedure. It is not known whether alveolar epithelium can present antigens to T lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
Characterization of space environmental effects on candidate solar sail material (2002)

The National Aeronautics and Space Administration's (NASA) Marshall Space Flight Center (MSFC) is concentrating research into the utilization of photonic materials for spacecraft propulsion. Spacecraft propulsion, using photonic materials, will be achieved using a solar sail. A sail operates on the principle that photons, originating from the sun, impart pressure and provide a source of spacecraft propulsion. The pressure can be increased, by a factor of two if the sun-facing surface is perfectly reflective. Solar sails are generally composed of a highly reflective metallic front layer, a thin polymeric substrate, and occasionally a highly emissive back surface. The Space Environmental Effects Team at MSFC is actively characterizing candidate solar sail materials to evaluate the thermo-optical and mechanical properties after exposure to a simulated Geosynchronous Transfer Orbit (GTO) radiation environment. This study is the first known characterization of solar sail materials exposed to space simulated environments. The technique of radiation dose verses material depth profiling was used to determine the orbital equivalent exposure doses. The solar sail exposure procedures and results of the material characterization will be discussed.
<i>Bacillus</i> Probiotics: Spore Germination in the Gastrointestinal Tract (2002)

Spores of Bacillus species are being used commercially as probiotics and competitive exclusion agents. Unlike the more commonly used Lactobacillus-type probiotics, spores are dormant life forms. To address how spore probiotics might function we have investigated whether spores can germinate in the gastrointestinal tract by using a murine model. Using a genetically engineered chimeric gene, ftsH-lacZ, which is strongly expressed only in vegetative cells, we have developed a sensitive competitive reverse transcription-PCR assay which has enabled detection of as few as 10(2) vegetative bacteria in the mouse gut. Using this method we have administered doses of ftsH-lacZ spores to groups of mice and shown that spores can germinate in significant numbers in the jejunum and ileum. The levels of detection we obtained suggest that spores may colonize the small intestine, albeit briefly.
Advances in two dimensional arrays for real time volumetric imaging (2002)

The authors have previously described prototype two-dimensional arrays used for real time volumetric imaging (1991, 1992, 1995). However, these arrays primarily operated at frequencies below 3.0 MHz, have been unwieldy to the operator due to the interconnect schemes used, and have taken up to 6 weeks to construct. The authors have recently constructed transducers operating at higher frequencies, and having a smaller interconnect geometry. These transducers include a 40/spl times/40=1600 element array operating at 3.5 MHz and a 60/spl times/60=3600 element array operating at 5.0 MHz for cardiac applications. Each transducer includes 256 transmit and 256 receive elements. The authors also constructed an 80/spl times/80=6400 element array operating at 2.5 MHz on a muti-layer polyimide connector for obstetric applications. This transducer has 256 transmit and 208 receive elements. Using a flex circuit interconnect, the authors reduced the size in the transducer assembly by a factor of four compared to their previous method of pin and socket interconnects. The transducer/handle assembly is integrated with the Duke University real time volumetric imaging system capable of generating multiple planes at any desired angle and depth within the pyramidal volume.