gebhart/patrepeval huggingface dataset:
Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus (1993)

Stable and potent fibrinolytic enzymes (six homogeneous proteins) were purified to homogeneity from extracts of the lyophilized powder of an earthworm, Lumbricus rubellus. The molecular weight of each enzyme estimated by SDS-polyacrylamide gel electrophoresis was different from those by gel filtration chromatography in the six purified proteins. The exact molecular weight of each enzyme (F-III-2, F-III-1, F-II, F-I-2, F-I-1, and F-I-0) measured by ion-spray MS analysis was 29,662, 29,667, 24,664, 24,220, 24,196, and 23,013, respectively. The isoelectric point (pI) of each enzyme was 3.40, 3.60, 4.20, 4.00, 4.30, and 4.85, respectively. The enzymes were single polypeptide chains. They had a very strong fibrinolytic activity and the maximum reactivity for chromogenic substrates from pH 9-11. The enzymes, acidic proteins that had abundant asparagine and aspartic acid, and low lysine in their amino acid composition, did not contain component sugars. The enzymes were stable at from pH 1-11 and up to 60 degrees C. Studies on substrate specificity and inhibition indicated that these enzymes were alkaline trypsin-like serine proteases. N-Terminal amino acid sequences of the enzymes had local similarities to those of trypsin-like enzymes such as elastase and coagulation factor IX. From the results of amino acid sequence, amino acid composition analyses and immunological analyses, it was suggested that these six enzyme proteins were derived as isozyme(s) from at least four different genes.
Demonstration of Real-Time Burst-Mode Digital Coherent Reception With Wide Dynamic Range in DSP-Based PON Upstream (2016)

This paper proposes a real-time burst-mode coherent receiver (BMCR) that enables wide dynamic range reception for future passive optical network (PON) applications, wherein two key burst-mode components are implemented; an automatic gain controlled semiconductor optical amplifier (AGC-SOA) for optical power equalization and a real-time digital signal processor (DSP) with a frame detection (FD) function. The AGC-SOA greatly increases the receiver dynamic range by reducing the impact of quantization errors in the analogue-to-digital conversion used for DSP-based signal demodulation while the FD function detects burst frame arrivals for frame-by-frame optimization of tap weights of an adaptive filter in the DSP. Successful real-time reception of 20 Gb/s single polarization quadrature phase shift keying burst signals with wide dynamic range of 22.0 dB is experimentally demonstrated. New system configurations of the DSP-based coherent PONs using the BMCR and wavelength plans assigned to the new systems are also proposed to offer a smooth migration from existing PONs.
Massive parallel-sequencing-based hydroxyl radical probing of RNA accessibility (2014)

Hydroxyl Radical Footprinting (HRF) is a tried-and-tested method for analysis of the tertiary structure of RNA and for identification of protein footprints on RNA. The hydroxyl radical reaction breaks accessible parts of the RNA backbone, thereby allowing ribose accessibility to be determined by detection of reverse transcriptase termination sites. Current methods for HRF rely on reverse transcription of a single primer and detection by fluorescent fragments by capillary electrophoresis. Here, we describe an accurate and efficient massive parallel-sequencing-based method for probing RNA accessibility with hydroxyl radicals, called HRF-Seq. Using random priming and a novel barcoding scheme, we show that HRF-Seq dramatically increases the throughput of HRF experiments and facilitates the parallel analysis of multiple RNAs or experimental conditions. Moreover, we demonstrate that HRF-Seq data for the Escherichia coli 16S rRNA correlates well with the ribose accessible surface area as determined by X-ray crystallography and have a resolution that readily allows the difference in accessibility caused by exposure of one side of RNA helices to be observed.
Malignant hyperthermia and excitation–contraction coupling (2001)

Malignant hyperthermia (MH) is a state of elevated skeletal muscle metabolism that may occur during general anaesthesia in genetically pre-disposed individuals. Malignant hyperthermia results from altered control of sarcoplasmic reticulum (SR) Ca2+ release. Mutations have been identified in MH-susceptible (MHS) individuals in two key proteins of excitation-contraction (EC) coupling, the Ca2+ release channel of the SR, ryanodine receptor type 1 (RyR1) and the alpha1-subunit of the dihydropyridine receptor (DHPR, L-type Ca2+ channel). During EC coupling, the DHPR senses the plasma membrane depolarization and transmits the information to the ryanodine receptor (RyR). As a consequence, Ca2+ is released from the terminal cisternae of the SR. One of the human MH-mutations of RyR1 (Arg614Cys) is also found at the homologous location in the RyR of swine (Arg615Cys). This animal model permits the investigation of physiological consequences of the homozygously expressed mutant release channel. Of particular interest is the question of whether voltage-controlled release of Ca2+ is altered by MH-mutations in the absence of MH-triggering substances. This question has recently been addressed in this laboratory by studying Ca2+ release under voltage clamp conditions in both isolated human skeletal muscle fibres and porcine myotubes.
Design of a smart leading edge device for low speed wind tunnel tests in the European project SADE (2011)

Purpose The purpose of this paper is to describe the pre‐design and sizing of a smart leading edge section which is developed in the project SADE (Smart High Lift Devices for Next Generation Wings), which is part of the seventh framework program of the EU. Design/methodology/approach The development of morphing technologies in SADE concentrates on the leading and trailing edge high‐lift devices. At the leading edge a smart gap and step‐less droop nose device is developed. For the landing flap a smart trailing edge of the flap is in the focus of the research activities. The main path in SADE follows the development of the leading edge section and the subsequent wind tunnel testing of a five meter span full‐scale section with a chord length of three meters in the wind tunnel T‐101 at the Russian central aero‐hydrodynamic institute (TsAGI) in Moscow. Findings The presented paper gives an overview over the desired performance and requirements of a smart leading edge device, its aerodynamic design for the wind tunnel tests and the structural pre‐design and sizing of the full‐scale leading edge section which will be tested in the wind tunnel. Originality/value SADE aims at a major step forward in the development and evaluation of the potential of morphing airframe technologies.
A Study of Micah 6.1–8 (1951)

It has often been remarked that the last verse of this passage is an epitome of the teaching of the three great eighth century prophets: the appeal of Amos for justice; Hosea's unfolding of the meaning of hesedh ; Isaiah's summons to the life of humble faith and obedience, which was the outcome of his vision of the holy and living God of Israel. Nowhere is the prophetic message more concisely and powerfully expressed. There are about these words a dignity and comprehensiveness which may easilytempt the modern reader to isolate them from what precedes, and so to miss the ground and the significance of the prophetic appeal. As George Adam Smith wrote many years ago: “Usually it is only the last of the verses upon which the admiration of the reader is bestowed: What doth the Lord require of thee, O man, but to do justice and love mercy and walk humbly with thy God ? But in truth the rest of the passage differeth not in glory; the wonder of it lies no more in its peroration than in its argument as a whole.” 1 The observation is salutary. We have not adequately interpreted these words (v. 8) when we see in them simply an ethical counterblast to a religion whose requirements were exclusively cultic and formal, or when we think of them as merely enunciating an austere but unattainable moral standard, as law without gospel. Nor ought they to be dismissed as an impressive testimony to a faith which we have outgrown.
Emotion Detection from text: A Survey (2014)

This survey describes recent works in the field of Emotion Detection from text, being a part of the broader area of Affective Computing. This survey has been inspired on the well-known fact that, despite there is a lot of work on emotional detection systems, a lot of work is expected to be done yet. The increment of these systems is due to the large amount of emotional data available in Social Web. Detecting emotions from text have attracted the attention of many researchers in computational linguistics because it has a wide range of applications, such as suicide prevention or measuring well-being of a community. This paper mainly collects works based on lexical and machine learning approaches and these works are classificated in accordance with the emotional model and the approach used.
Efficient Geometry Compression for GPU‐based Decoding in Realtime Terrain Rendering (2009)

Abstract We present a geometry compression scheme for restricted quadtree meshes and use this scheme for the compression of adaptively triangulated digital elevation models (DEMs). A compression factor of 8–9 is achieved by employing a generalized strip representation of quadtree meshes to incrementally encode vertex positions. In combination with adaptive error‐controlled triangulation, this allows us to significantly reduce bandwidth requirements in the rendering of large DEMs that have to be paged from disk. The compression scheme is specifically tailored for GPU‐based decoding, since it minimizes dependent memory access operations. We can thus trade CPU operations and CPU–GPU data transfer for GPU processing, resulting in twice faster streaming of DEMs from main memory into GPU memory. A novel storage format for decoded DEMs on the GPU facilitates a sustained rendering throughput of about 300 million triangles per second. Due to these properties, the proposed scheme enables scalable rendering with respect to the display resolution independent of the data size. For a maximum screen‐space error below 1 pixel it achieves frame rates of over 100 fps, even on high‐resolution displays. We validate the efficiency of the proposed method by presenting experimental results on scanned elevation models of several hundred gigabytes.
Coding Cell Micropatterns Through Peptide Inkjet Printing for Arbitrary Biomineralized Architectures (2018)

Well-designed micropatterns present in native tissues and organs involve changes in extracellular matrix compositions, cell types and mechanical properties to reflect complex biological functions. However, the design and fabrication of these micropatterns <i>in vitro</i> to meet task-specific biomedical applications remains a challenge. A <i>de novo</i> design strategy to code and synthesize functional micropatterns is presented to engineer cell alignment through the integration of aqueous-peptide inkjet printing and site-specific biomineralization. The inkjet printing provides direct writing of macroscopic biosilica selective peptide-R5 patterns with micrometer-scale resolution on the surface of a biopolymer (silk) hydrogel. This is combined with <i>in situ</i> biomineralization of the R5 peptide for site-specific growth of silica nanoparticles on the micropatterns, avoiding the use of harsh chemicals or complex processing. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin. This combination of peptide printing and site-specific biomineralization provides a new route for developing cost-effective micropatterns, with implications for broader materials designs. <b>Coding cell micropatterns through peptide inkjet printing for arbitrary biomineralized architectures</b> is demonstrated here. The functional micropatterned systems are used to align human mesenchymal stem cells and bovine serum albumin <i>in vitro</i>, avoiding the use of harsh chemicals or complex processing, while providing potential applications in developing cost-effective micropatterns to meet task-specific biomedical applications.
Rapid development of tightly-coupled GPS/INS systems (1997)

This paper addresses the question: "Why aren't tightly-coupled OPS/INS systems everywhere, on aircraft, ships and land vehicles?" Two barriers to the widespread use are cited. One is the high cost of the INS, and the other is the cost and complexity of tightly-coupled OPS/INS integration. One of those two barriers has recently been diminished drastically with the development of a standardized software package for tightly-coupled integration. In the past, only the largest corporations have been able to pay the initial development cost for tightly-coupled OPS/INS integration, usually with funding from a large defense program. Using the new software package, integration and van test can be accomplished in a matter of days, and this has been demonstrated with field trials. The package is intended primarily for small companies that otherwise would not be able to build tightly-coupled OPS/INS systems at all. What would have been a prohibitive 3- or 4-man year development effort is reduced to a few man weeks. To accomplish an integration, the system integrator has to find a way, through serial interfaces or by some other means, to get the INS measurements of acceleration (accumulated velocity change /spl Delta/V) and attitude rate (accumulated angle change /spl Delta//spl theta/) into a processor, along with the raw data of a GPS receiver. He also has to find a way to time tag the INS /spl Delta/V, /spl Delta//spl theta/ with GPS time. The rest of tightly-coupled OPS/INS integration is predominately accomplished in the standardized software package. That leaves the cost of the INS as the only remaining barrier to the very widespread use of OPS/INS, and invites new development of low cost inertial sensors. The focus of this paper is on the software package, and how it achieves standardization and ease of use while retaining the flexibility to produce optimal results with a variety of INS and GPS receiver types.
Ligand-Specific Structural Changes in the Vitamin D Receptor in Solution (2011)

Vitamin D receptor (VDR) is a member of the nuclear hormone receptor superfamily. When bound to a variety of vitamin D analogues, VDR manifests a wide diversity of physiological actions. The molecular mechanism by which different vitamin D analogues cause specific responses is not understood. The published crystallographic structures of the ligand binding domain of VDR (VDR-LBD) complexed with ligands that have differential biological activities have exhibited identical protein conformations. Here we report that rat VDR-LBD (rVDR-LBD) in solution exhibits differential chemical shifts when bound to three ligands that cause diverse responses: the natural hormone, 1,25-dihydroxyvitamin D(3) [1,25(OH)₂D₃], a potent agonist analogue, 2-methylene-19-nor-(20S)-1,25-dihydroxyvitamin D₃ [2MD], and an antagonist, 2-methylene-(22E)-(24R)-25-carbobutoxy-26,27-cyclo-22-dehydro-1α,24-dihydroxy-19-norvitamin D₃ [OU-72]. Ligand-specific chemical shifts mapped not only to residues at or near the binding pocket but also to residues remote from the ligand binding site. The complexes of rVDR-LBD with native hormone and the potent agonist 2MD exhibited chemical shift differences in signals from helix-12, which is part of the AF2 transactivation domain that appears to play a role in the selective recruitment of coactivators. By contrast, formation of the complex of rVDR-LBD with the antagonist OU-72 led to disappearance of signals from residues in helices-11 and -12. We present evidence that disorder in this region of the receptor in the antagonist complex prevents the attachment of coactivators.
Convenient preparation of B-trichloroborazine (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConvenient preparation of B-trichloroborazineEugene F. Rothgery and L. F. HohnstedtCite this: Inorg. Chem. 1967, 6, 5, 1065–1066Publication Date (Print):May 1, 1967Publication History Published online1 May 2002Published inissue 1 May 1967https://pubs.acs.org/doi/10.1021/ic50051a049https://doi.org/10.1021/ic50051a049research-articleACS PublicationsRequest reuse permissionsArticle Views301Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium in two US Great Basin hot springs with abundant ammonia‐oxidizing archaea (2011)

Many thermophiles catalyse free energy-yielding redox reactions involving nitrogenous compounds; however, little is known about these processes in natural thermal environments. Rates of ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium (DNRA) were measured in source water and sediments of two ≈ 80°C springs in the US Great Basin. Ammonia oxidation and denitrification occurred mainly in sediments. Ammonia oxidation rates measured using (15)N-NO(3)(-) pool dilution ranged from 5.5 ± 0.8 to 8.6 ± 0.9 nmol N g(-1) h(-1) and were unaffected or only mildly stimulated by amendment with NH(4) Cl. Denitrification rates measured using acetylene block ranged from 15.8 ± 0.7 to 51 ± 12 nmol N g(-1) h(-1) and were stimulated by amendment with NO(3)(-) and complex organic compounds. The DNRA rate in one spring sediment measured using an (15)N-NO(3)(-) tracer was 315 ± 48 nmol N g(-1) h(-1). Both springs harboured distinct planktonic and sediment microbial communities. Close relatives of the autotrophic, ammonia-oxidizing archaeon 'Candidatus Nitrosocaldus yellowstonii' represented the most abundant OTU in both spring sediments by 16S rRNA gene pyrotag analysis. Quantitative PCR (qPCR) indicated that 'Ca. N. yellowstonii'amoA and 16S rRNA genes were present at 3.5-3.9 × 10(8) and 6.4-9.0 × 10(8) copies g(-1) sediment. Potential denitrifiers included members of the Aquificales and Thermales. Thermus spp. comprised <1% of 16S rRNA gene pyrotags in both sediments and qPCR for T. thermophilus narG revealed sediment populations of 1.3-1.7 × 10(6) copies g(-1) sediment. These data indicate a highly active nitrogen cycle (N-cycle) in these springs and suggest that ammonia oxidation may be a major source of energy fuelling primary production.
A unified framework for augmented reality and knowledge-based systems in maintaining aircraft (2014)

Aircraft maintenance and training play one of the most important roles in ensuring flight safety. The maintenance process usually involves massive numbers of components and substantial procedural knowledge of maintenance procedures. Maintenance tasks require technicians to follow rigorous procedures to prevent operational errors in the maintenance process. In addition, the maintenance time is a cost-sensitive issue for airlines. This paper proposes intelligent augmented reality (IAR) system to minimize operation errors and time-related costs and help aircraft technicians cope with complex tasks by using an intuitive UI/UX interface for their maintenance tasks. The IAR system is composed mainly of three major modules: 1) the AR module 2) the knowledge-based system (KBS) module 3) a unified platform with an integrated UI/UX module between the AR and KBS modules. The AR module addresses vision-based tracking, annotation, and recognition. The KBS module deals with ontology-based resources and context management. Overall testing of the IAR system is conducted at Korea Air Lines (KAL) hangars. Tasks involving the removal and installation of pitch trimmers in landing gear are selected for benchmarking purposes, and according to the results, the proposed IAR system can help technicians to be more effective and accurate in performing their maintenance tasks.
Evaluation of hot carrier degradation of N-channel MOSFET's with the charge pumping technique (1986)

Hot carrier degradation in n-channel MOSFET transistors has been evaluated using the charge pumping technique in addition to the conventional <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I_{ds}-V_{gs}</tex> measurements. It is shown that the generation of large amounts of interface states is the primary result of moderate hot carrier stress. The simultaneous injection and recombination of injected electrons and holes is suggested to be responsible for this formation of interface states. The net charge in the oxide and interface states after any hot carrier stress is shown to be positive.
Olaparib combined with abiraterone in patients with metastatic prostate cancer: Safety run-in from a phase II study. (2015)

e16026 Background: Olaparib (Lynparza), a PARP inhibitor with antitumor activity in germline (g) BRCA1/2 mutation-associated breast and ovarian cancer, has indicated antitumor activity in gBRCA1/2 mutation-associated prostate cancer (Kaufman et al. JCO 2014). Abiraterone is approved for patients with metastatic castration-resistant prostate cancer (mCRPC) before chemotherapy and after docetaxel-based chemotherapy. Reported here is an open-label safety run-in assessment (part A) from a randomized, placebo-controlled Phase II study (NCT01972217) of olaparib in combination with abiraterone. Methods: Patients (pts) with mCRPC in prior receipt of docetaxel-based chemotherapy were initially administered olaparib 200 mg twice daily (bid) and abiraterone 1000 mg once daily (qd). After 3 pts had received ≥ 14 days’ treatment with both olaparib and abiraterone, dose-limiting toxicities (DLTs) were assessed by a safety review committee (SRC). If ≤ 1 DLT was observed, either olaparib was escalated to 300 mg bid alone for 3–7 days then combined with abiraterone 1000 mg qd (group 1), or abiraterone 1000 mg qd was administered alone for 5–7 days then in combination with olaparib 300 mg bid (group 2). Prednisone or prednisolone 5 mg bid was administered with abiraterone throughout. Results: In total, 16 pts enrolled. In the 3 pts receiving olaparib 200 mg bid and abiraterone 1000 mg qd, no DLTs were observed. In the 13 pts (group 1, n = 7; group 2, n = 6) subsequently dosed with olaparib 300 mg bid and abiraterone 1000 mg qd, no DLTs were observed in 9 who completed > 2 weeks of treatment, 3 were still receiving treatment ( < 2 weeks) and 1 (group 1) withdrew consent following an adverse event (AE) of grade 1 nausea, which was not considered a DLT by the SRC. At the time of reporting 41 AEs had been observed in 10 pts, of which nausea occurred most frequently (5 pts), followed by vomiting and loose stools (3 pts each). No serious AEs considered related to olaparib were observed. Conclusions: DLTs were not observed in any pt dosed with olaparib 200 mg bid (n = 3) or 300 mg bid (n = 9). Olaparib 300 mg bid was considered an appropriate dose for further study in combination with abiraterone in pts with mCRPC. Clinical trial information: NCT01972217.
Comparison of Combined Barker Codes for Coded Radar Use (1967)

The autocorrelation functions øI(τ) and øII(τ) of Codes I and II are shown in Figs. 1 and 2, respectively. Several interesting points are noted from a comparison of these figures.