gebhart/patrepeval huggingface dataset:
Precise Phase Transition Measurements of Organic Materials by Differential Thermal Analysis (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrecise Phase Transition Measurements of Organic Materials by Differential Thermal AnalysisD. A. Vassallo and J. C. HardenCite this: Anal. Chem. 1962, 34, 1, 132–135Publication Date (Print):January 1, 1962Publication History Published online1 May 2002Published inissue 1 January 1962https://doi.org/10.1021/ac60181a040RIGHTS & PERMISSIONSArticle Views97Altmetric-Citations57LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (474 KB) Get e-Alerts Get e-Alerts
Phosphoinositide-specific phospholipase C-.delta.1: effect of monolayer surface pressure and electrostatic surface potentials on activity (1992)

We added phospholipase C-delta 1 (PLC-delta) to the aqueous subphase beneath monolayers formed from mixtures of phosphatidylinositol 4,5-bisphosphate (2% PIP2), phosphatidylserine (33% PS), and phosphatidylcholine (65% PC) and then measured the initial rate of hydrolysis of PIP2 after addition of 10 microM free calcium. Increasing the surface pressure of the monolayer, pi, from 20 to 40 mN/m decreased the rate of hydrolysis 200-fold. The rate of hydrolysis depends exponentially on the surface pressure: rate alpha exp(-pi Ap/kT) where k is the Boltzmann constant, T is the temperature, and Ap congruent to 1 nm2. Similar results were obtained with different (1 and 100 microM) free [Ca2+] and with different mole fractions of PIP2. The results are consistent with a model in which PLC-delta binds to PIP2 with high affinity (Ka = 10(6) M-1) in the absence of calcium ions [Rebecchi, M.J., Peterson, A., & McLaughlin, S. (1993) Biochemistry (preceding paper in this issue)], and a portion of PLC-delta of area Ap inserts into the monolayer doing work = pi Ap prior to hydrolysis of PIP2. Removing the monovalent acidic lipid PS from the monolayer decreases the activity of PLC-delta 4-fold, this effect of PS on activity is similar to the effect of monovalent acidic lipids on the binding of PLC-delta to PIP2 in bilayer vesicles.
Concentration of Different Minerals and Bio Availability of Iron of the Selected Varieties of Groundnuts (1995)

Groundnut is considered. The King of oil seeds' as it is one of the main source of edibie oil in the country. The average pod yieid of groundnuts in india varies from 700 to 900 kg per hectare.
Development of a lithium microbattery packaging technology: ERKTS01. Final report (1995)

The objective of this joint project between Oak Ridge National Laboratory (ORNL) and Eveready Battery Company (EBC) was to develop a coating process that would protect the lithium anode of thin-film rechargeable lithium batteries from air. Several methods were investigated including metallization of the lithium film, coverage of the lithium anode with the electrolyte, lithium phosphorus oxynitride (Lipon), and other ceramic films, and a multilayer coating consisting of alternating films of parylene and metal and/or ceramic films. The parylene-ceramic or metal multilaver coating was found to be an effective packaging method for thin-film lithium batteries.
A Changing Pattern of Brain-Derived Neurotrophic Factor Expression Correlates with the Rearrangement of Fibers during Cochlear Development of Rats and Mice (1999)

The reorganization of specific neuronal connections is a typical feature of the developing nervous system. It is assumed that the refinement of connections in sensory systems requires spontaneous activity before the onset of cochlear function and selective sensory experience during the ensuing period. The mechanism of refinement through sensory experience is currently postulated as being based on the selective reinforcement of active projections by neurotrophins. We studied a presumed role of neurotrophins for rearrangement of afferent and efferent fibers before the onset of sensory function in the precisely innervated auditory end organ, the cochlea. We observed a spatiotemporal change in the localization of brain-derived neurotrophic factor (BDNF) protein and mRNA, which correlated with the reorganization of fibers. Thus, BDNF decreased in target hair cells during fiber retraction and was subsequently upregulated in neurons, target hair cells, and adjacent supporting cells concomitant with the formation of new synaptic contacts. Analysis of the innervation pattern in BDNF gene-deleted mice by immunohistochemistry and confocal microscopy revealed a failure in the rearrangement of fibers and a BDNF dependency of distinct neuronal projections that reorganize in control animals. Our data suggest that, before the onset of auditory function, a spatiotemporal change in BDNF expression in sensory, epithelial, and neuronal cells may guide the initial steps of refinement of the innervation pattern.
Quasi‐three‐dimensional modeling of rip current systems (2003)

The focus of the paper is the analysis of the flow in rip current systems generated by channels in longshore bars on a beach. The horizontal variations of rip current systems are described through the use of the quasi‐three‐dimensional nearshore circulation model SHORECIRC. Model predictions are compared to laboratory measurements of waves and current velocities throughout the entire rip current system and show reasonable agreement. The rips in the two channels are found to behave differently because of the depth variation across the basin. It is found that higher bottom stress leads to more stable flow where the rip current meanders less and fewer eddies are generated. The wave current interaction creates forcing which reduces the distance rip currents flow offshore and can lead to a slow pulsation of the rip current. This pulsation is in addition to the instabilities of a jet which can also be present in rip currents. The three dimensionality of the rip current system is found to have a significant effect on the overall circulation patterns.
From the Background to the Spotlight: TASK Channels in Pathological Conditions (2010)

TWIK-related acid-sensitive potassium channels (TASK1-3) belong to the family of two-pore domain (K(2P) ) potassium channels. Emerging knowledge about an involvement of TASK channels in cancer development, inflammation, ischemia and epilepsy puts the spotlight on a leading role of TASK channels under these conditions. TASK3 has been especially linked to cancer development. The pro-oncogenic potential of TASK3 could be shown in cell lines and in various tumor entities. Pathophysiological hallmarks in solid tumors (e.g. low pH and oxygen deprivation) regulate TASK3 channels. These conditions can also be found in (autoimmune) inflammation. Inhibition of TASK1,2,3 leads to a reduction of T cell effector function. It could be demonstrated that TASK1(-/-) mice are protected from experimental autoimmune inflammation while the same animals display increased infarct volumes after cerebral ischemia. Furthermore, TASK channels have both an anti-epileptic as well as a pro-epileptic potential. The relative contribution of these opposing influences depends on their cell type-specific expression and the conditions of the cellular environment. This indicates that TASK channels are per se neither protective nor detrimental but their functional impact depends on the "pathophysiological" scenario. Based on these findings TASK channels have evolved from "mere background" channels to key modulators in pathophysiological conditions.
Special Stains in the Microwave Oven (1986)

We describe the modification of a number of stains for the microwave oven: Grocott's methenamine silver, acid fast bacteria, Giemsa, Lillie's melanin, methyl green pyronin, Masson's trichrome, and glycogen double digestion. Their adaptation has markedly reduced the time needed for staining. The staining procedures described here can be used on paraffin sections, frozen sections on coated slides, touch preparations, and cytology specimens.
Plasma Trapping in Cusped Geometries (1960)

Received 10 November 1959DOI:https://doi.org/10.1103/PhysRevLett.4.222©1960 American Physical Society
Alkali Denaturation of Covalently Closed Circular Duplex Deoxyribonucleic Acid (1970)

Abstract The alkaline pH range over which the double stranded circular DNA of the replicative form of the bacterial virus, ϕX174, is reversibly denatured has been determined in order to specify accurately the conditions for a preparative procedure for this DNA based on selective alkali denaturation. The denaturation remains entirely reversible to pH 12.6 at an ionic strength of about 0.25 over a range in which the sedimentation coefficient, after an initial drop, more than doubles. The sharp transition above pH 12.6 in which the denaturation becomes completely irreversible is accompanied by only a small further increase in sedimentation coefficient. Sedimentation-pH titration curves have been determined in detail for the replicative form of ϕX174 (molecular weight 3.4 x 106 daltons) and for the circular DNA of a penicillinase plasmid of Staphylococcus aureus (molecular weight 16.5 x 106 daltons). The latter curve is displaced to a lower pH and the two phase nature of the curve in the range of increasing sedimentation coefficient is markedly accentuated.
Leukocyte colony-stimulating factors. A review of associated neutrophilic dermatoses and vasculitides (1994)

Serious cutaneous adverse effects of colony-stimulating factors are distinctly rare but include neutrophilic dermatoses and necrotizing vasculitis. Upregulation of neutrophilic function and secondary release of cytokines may induce these complications. Early recognition and cessation of colony-stimulating factor therapy will limit the morbidity of these adverse events.
The Outsourcing Strategy of Local and Multinational Firms: A Supply Base Perspective (2014)

Firms outsource through connecting to local and global supply bases and making such connections produces costs of search and evaluation, which are a function of transaction characteristics and firm capabilities. We argue that firms outsource more when those costs are low. Hence, domestic subsidiaries of multinational firms, with low cost access to both local and global supply bases, outsource more than either domestic firms or foreign subsidiaries, as confirmed by evidence from a large data panel. We also propose that among foreign subsidiaries, distance from the home country co‐determines search and evaluation costs such that subsidiaries from more distant countries outsource less. This is confirmed for geographic distance, but a positive effect is found for political distance and a mixed effect for cultural distance.
Inositol 6 g daily may be effective in depression but not in schizophrenia (1993)

Abstract Inositol is an important precursor for second messenger synthesis and has been reported to be reduced by lithium treatment in rat brain and in human CSF in depression. An open trial of 6 g/day in 11 depressed patients resistant to previous treatment led to major improvement in nine patients. The enzyme synthesizing inositol has been reported to be elevated in schizophrenia, suggesting an attempted compensation for possible inositol deficiency. A controlled double‐blind crossover trial in 10 chronic schizophrenic patients of 6 g/day of inositol for 30 days did not reveal any benefit.
A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. (1992)

Fission yeast centromeres vary in size but are organized in a similar fashion. Each consists of two distinct domains, namely, the approximately 15-kilobase (kb) central region (cnt+imr), containing chromosome-specific low copy number sequences, and 20- to 100-kb outer surrounding sequences (otr) with highly repetitive motifs common to all centromeres. The central region consists of an inner asymmetric sequence flanked by inverted repeats that exhibit strict identity with each other. Nucleotide changes in the left repeat are always accompanied with the same changes in the right. The chromatin structure of the central region is unusual. A nucleosomal nuclease digestion pattern formed on unstable plasmids but not on stable chromosome. DNase I hypersensitive sites correlate with the location of tRNA genes in the central region. Autonomously replicating sequences are also present in the central region. The behavior of truncated minichromosomes suggested that the central region is essential, but not sufficient, to confer transmission stability. A portion of the outer repetitive region is also required. A larger outer region is necessary to ensure correct meiotic behavior. Fluorescence in situ hybridization identified individual cens. In the interphase, they cluster near the nuclear periphery. The central sequence (cnt+imr) may play a role in positioning individual chromosomes within the nucleus, whereas the outer regions (otr) may interact with each other to form the higher-order complex structure.
Isoelectric pH of polyamide–epichlorohydrin modified soy protein improved water resistance and adhesion properties (2006)

Abstract Protein macromolecules derived from plants have been considered as alternative resources for various applications, including adhesives, films, rubbers, and biocomposites. Plant protein polymers are pH sensitive and need to be modified to meet application performance. This study demonstrated interactions between polyamide–epichlorohydrin (PAE) and soy protein as affected by pH and temperature. PAE and soy protein molecules formed reversible ionic complexes at room temperature at a pH range of 4–9. The complexation interactions acted as physical crosslinking, which stabilized the soy protein structure and increased its denaturation temperature and enthalpy. The viscosity of adhesives derived from the interaction of PAE and soy protein was affected significantly by the complexation formation, denaturation, and pH. The complexation interactions improved the adhesion properties of the PAE/modified soy protein. pH also played an important role in the adhesion performance, which was attributed to the pH dependence of the protein conformation and PAE/soy protein complexation interactions. © 2006 Wiley Periodicals, Inc. J Appl PolymSci 103: 2261–2270, 2007
Broadband design of a low‐profile reflector antenna (2013)

This work presents the design and analysis of a modified Fresnel zone plate reflector for broadband operation. A new technology based on slight deformations on a flat metallic surface is applied to obtain the geometrical model of the proposed flat reflector antenna. In comparison with reflectarrays, Fresnel zone reflectors are lossless, low cost, easy to fabricate and broadband. According to the specifications of a previously published reflectarray, a centre‐fed prototype whose dimensions are 28 × 21 cm has been designed and analysed showing a maximum gain of 28.06 dBi at 12.5 GHz and a 32.8% 3 dB gain bandwidth in the range between 10.4 and 14.5 GHz. A second prototype has been designed and analysed to evaluate the performance of the proposed design method compared with a previous design of a Fresnel zone reflector. Better results in terms of gain and antenna efficiency have been obtained.
IMP dehydrogenase from Pneumocystis carinii as a potential drug target (1997)

Mycophenolic acid, a specific inhibitor of IMP dehydrogenase (IMPDH; EC 1.1.1.205), is a potent inhibitor of Pneumocystis carinii growth in culture, suggesting that IMPDH may be a sensitive target for chemotherapy in this organism. The IMPDH gene was cloned as a first step to characterizing the enzyme and developing selective inhibitors. A 1.3-kb fragment containing a portion of the P. carinii IMPDH gene was amplified by PCR with two degenerate oligonucleotides based on conserved sequences in IMPDH from humans and four different microorganisms. Northern hybridization analysis showed the P. carinii IMPDH mRNA to be approximately 1.6 kb. The entire cDNA encoding P. carinii IMPDH was isolated and cloned. The deduced amino acid sequence of P. carinii IMPDH shared homology with bacterial (31 to 38%), protozoal (48 to 59%), mammalian (60 to 62%), and fungal (62%) IMPDH enzymes. The IMPDH cDNA was expressed by using a T7 expression system in an IMPDH-deficient strain of Escherichia coli (strain S phi 1101). E. coli S phi 1101 cells containing the P. carinii IMPDH gene were able to grow on medium lacking guanine, implying that the protein expressed in vivo was functional. Extracts of these E. coli cells contained IMPDH activity that had an apparent Km for IMP of 21.7 +/- 0.3 microM and an apparent Km for NAD of 314 +/- 84 microM (mean +/- standard error of the mean; n = 3), and the activity was inhibited by mycophenolic acid (50% inhibitory concentration, 24 microM; n = 2).
Immunocytochemical study of the binding of fibrinogen and thrombospondin to ADP- and thrombin-stimulated human platelets (1985)

We have used immunogold staining to locate thrombospondin (TSP) on thrombin-activated human platelets, and have compared its distribution with that of fibrinogen (or fibrin) on thrombin- and ADP-stimulated platelets. To do this, isolated platelets were incubated with monospecific antibodies to TSP or fibrinogen (fib) and the bound IgG located with a second antibody adsorbed to gold particles. Thrombin- induced secretion in Tyrode-Ca2+ was followed by both anti-TSP and anti- fib binding, with large clusters of gold particles observed on the platelet surface. Little or no labeling was observed on unstimulated platelets with either antibody. When secretion was effected in Tyrode- EDTA, anti-TSP IgG still bound to the activated platelets, but the number of particle clusters was significantly reduced. Little binding of anti-fib IgG now occurred. Platelets activated with ADP in the presence of added fib, and subsequently incubated with anti-fib IgG, showed small particle clusters over the whole platelet surface. Thrombin-stimulated platelets from two patients with thrombasthenia bound anti-TSP IgG similarly to normal platelets activated in Tyrode- EDTA. No anti-fib binding occurred. Our results suggest that fib and TSP bind to specific domains on the stimulated platelet membrane. Such sites may be responsible for the mediation of platelet surface contact interactions.
Construction of an extended three-dimensional idiotope map by electron microscopic analysis of idiotope-anti-idiotope complexes. (1987)

A three-dimensional map of the positions of four idiotypic determinants (idiotopes or Ids) and an isotypic determinant was derived by transmission electron microscopy of negatively stained immune complexes. Each complex was composed of a monoclonal Id-expressing IgG and one or two varieties of monoclonal anti-Id (or anti-isotype) Fab fragment or IgG. Data from the various combinations of Id and anti-Id (and anti-isotype) were used to construct a low-resolution three-dimensional model that revealed not only the approximate locations of Ids on the surface of the antibody variable domains but also details of the geometry of Id-anti-Id interactions not otherwise available. The Ids were shown to be dispersed over the variable domains, extending from the complementarity-determining region to near the variable-constant switch region. Thus, immunoelectron microscopy is a useful complement to serologic, biochemical, and genetic strategies for the topographical analysis of immunoglobulin Ids or other epitopes. This same approach should be of broader applicability in the study of epitopes and receptor sites on other macromolecules.
CFD assessment of orifice aspect ratio and mass flow ratio on jet mixing in rectangular ducts (1994)

Isothermal CFD analysis was performed on axially opposed rows of jets mixing with cross flow in a rectangular duct. Laterally, the jets' centerlines were aligned with each other on the top and bottom walls. The focus of this study was to characterize the effects of orifice aspect ratio and jet-to-mainstream mass flow ratio on jet penetration and mixing. Orifice aspect ratios (L/W) of 4-to-1, 2-to-1, and 1-to-1, along with circular holes, were parametrically analyzed. Likewise, jet-to-mainstream mass flow ratios (MR) of 2.0, 0.5, and 0.25 were systematically investigated. The jet-to-mainstream momentum-flux ratio (J) was maintained at 36 for all cases, and the orifice spacing-to-duct height (S/H) was varied until optimum mixing was attained for each configuration. The numerical results showed that orifice aspect ratio (and likewise orifice blockage) had little effect on jet penetration and mixing. Based on mixing characteristics alone, the 4-to-1 slot was comparable to the circular orifice. The 4-to-1 slot has a smaller jet wake which may be advantageous for reducing emissions. However, the axial length of a 4-to-1 slot may be prohibitively long for practical application, especially for MR of 2.0. The jet-to-mainstream mass flow ratio had a more significant effect on jet penetration and mixing. For a 4-to-1 aspect ratio orifice, the design correlating parameter for optimum mixing (C = (S/H)(sq. root J)) varied from 2.25 for a mass flow ratio of 2.0 to 1.5 for a mass flow ratio of 0.25.
Naming page elements in end-user web automation (2005)

The names of commands and objects are vital to the usability of a programming system. We are developing a web automation system in which users need to identify web page elements, such as hyperlinks and form fields, in pages written by other designers. Using a survey of 40 users asking them to provide names for page elements, we found that users' names varied widely. However, when names were restricted to using only visible words from the web page. we were able to develop name resolution techniques that automatically find the desired page element given the user's name for it, striking a balance between usability and the precision required by the programming system.
Gold corroles (2011)

Although a variety of conditions proved unsuccessful for gold insertion into β-unsubstituted meso-triarylcorroles, refluxing free-base β-octabromo-meso-triarylcorrole ligands, H 3 [Br 8 T (p- X-P)C] ( X = MeO , n- Bu , CH 3 , H , and CF 3 ), with chloroauric acid in dichloromethane containing a small amount of triethylamine for 30 min led to 54–65% yields of the corresponding gold complexes.