gebhart/patrepeval huggingface dataset:
Quantitative assays of epidermal growth factor receptor in human breast cancer: Cut‐off points of clinical relevance (1988)

Epidermal growth factor receptor (EGFr) assays were performed by 3 different methods on 246 human primary breast carcinomas. Scatchard analyses of multipoint binding data on 19 of the first 209 tumours, performed by a displacement method, demonstrated that the majority of tumours exhibited 2 classes of binding site, the high-affinity site with an affinity constant (KD) of mean 2 nmol/l (SD 1.3, range 0.44-7 nmol/l), and a low-affinity site, KD mean 9.5 nmol/l (range 6-15.5 nmol/l). Scatchard analysis of multipoint assays using increasing concentrations of 125I-labelled EGF showed that saturation of the high-affinity site occurred in the majority of saturation of the high-affinity site occurred in the majority of tumours at a concentration of labelled EGF of I nM. Comparison of the KD values of the high-affinity site obtained from displacement assays with those obtained by increasing labelled EGF showed that the KD was significantly higher (p = 0.0002) when measured by the latter method. There was no difference in binding capacity of the high-affinity or low-affinity sites by the 2 methods. A 2-point assay with I nM labelled EGF (specific activity approx. 80-130 microCi/microgram) correlated with quantitative values for the high-affinity site from Scatchard analysis (p less than 0.02). There was a strong inverse relationship between EGFr greater than 10 fmol/mg membrane protein (2-point assay) and ER (dextran-coated charcoal method), Chi-squared 2 X 2 contingency table test = 34.027, p less than 0.0001. Follow-up data from 135 patients revealed a strong inverse relationship between EGFr greater than 10 fmol/mg membrane protein and oestrogen receptor (ER) status and a positive correlation with early recurrence and death. Our data describe a reproducible assay for EGFr and show that a cut-off point at 10 fmol/mg allows clinically useful application.
Online Buffer Fullness Estimation Aided Adaptive Media Playout for Video Streaming (2011)

Adaptive media playout (AMP) control is proposed in order to compensate for the bit-rate fluctuation of networks, which may result in playout interruptions in video streaming application. Most AMP algorithms found in the literature trigger playout-rate adjustments based on the buffer fullness or its variation. However, the challenge of the threshold based methods is to select the appropriate threshold for triggering a playout-rate adjustment owing to the unknown fluctuation of the channel quality and the video bitrate. We conceive an adaptive media playout regime based on underflow probability estimation, which requires no significant statistical knowledge of the previous tele-traffic load. To achieve this, we present an underflow probability estimation model based on large deviation theory relying on the buffer fullness and on its variation. We will then directly use the underflow probability to trigger the actions of playout control, instead of using indirect methods based on a buffer fullness threshold or buffer fullness variation threshold. Experiments based on MPEG-4 Variable Bit-Rate encoded video and VBR channels associated with Adaptive Modulation and Coding are conducted in order to investigate the achievable performance of the proposed algorithm. Our simulation results demonstrate an improved performance in comparison to other recent AMP algorithms.
Providing Fault Detection from Sensor Data in Complex Machines That Build the Smart City (2022)

Household appliances, climate control machines, vehicles, elevators, cash counting machines, etc., are complex machines with key contributions to the smart city. Those devices have limited memory and processing power, but they are not just actuators; they embed tens of sensors and actuators managed by several microcontrollers and microprocessors communicated by control buses. On the other hand, predictive maintenance and the capability of identifying failures to avoid greater damage of machines is becoming a topic of great relevance in Industry 4.0, and the large amount of data to be processed is a concern. This article proposes a layered methodology to enable complex machines with automatic fault detection or predictive maintenance. It presents a layered structure to perform the collection, filtering and extraction of indicators, along with their processing. The aim is to reduce the amount of data to work with, and to optimize them by generating indicators that concentrate the information provided by data. To test its applicability, a prototype of a cash counting machine has been used. With this prototype, different failure cases have been simulated by introducing defective elements. After the extraction of the indicators, using the Kullback-Liebler divergence, it has been possible to visualize the differences between the data associated with normal and failure operation. Subsequently, using a neural network, good results have been obtained, being able to correctly classify the failure in 90% of the cases. The result of this application demonstrates the proper functioning of the proposed approach in complex machines.
The Customs House (2012)

Andrew Motion's new book opens with a sequence of war poems (first published as the pamphlet Laurels and Donkeys, on Armistice Day 2010), drawing on soldiers' experiences of war from 1914 until today - beginning with a story about Siegfried Sassoon and moving via World War Two and Korea to the recent conflicts in Iraq and Afghanistan. Many of the poems are in the voices of combatants, others are based on memories of the poet's father, who landed at D-day and fought in France and Germany. poems combine understatement with a clear-eyed and unswerving candor. The Customs House has other rooms: a group of topographies, mapping moments in a marriage against the contingencies of place and family history; and several 'found poems', in which the poet collaborates with his source, mixing what is there already with what is about to be there: whether a remarkable sonnet sequence on the last days of the Baroque genius Francesco Borromini, or in other poems a richly imagined extrapolation from the silent premises of a painting.
Aluminum Silicon Carbide (AlSiC) for Advanced Microelectronic Packages (1998)

Aluminum Silicon Carbide (AlSiC) metal matrix composite (MMC) materials have a unique set of material properties that are ideally suited for all electronic packaging applications requiring thermal management. The AlSiC coefficient of thermal expansion (CTE) value is compatible with direct IC device attachment for the maximum thermal dissipation through the 170 – 200 W/mK thermal conductivity value material. Additionally, the low material density of AlSiC makes it ideal for weight sensitive applications such as portable devices. The AlSiC material and functional electronic packaging geometries are produced using the Ceramics Process Systems Corporation's QuickSet™/QuickCast™ net-shape (without machining) fabrication process. Additionally, functional packaging features such as feedthrus, sealrings and substrates are incorporated in AlSiC during fabrication using the CPS Concurrent Integration™ technique eliminating the need for the traditional assembly and brazing (or soldering) operations. The ideal AlSiC material properties and the AlSiC net-shape fabrication process provides low-cost high performance functional thermal management packaging solutions. This paper will review the material properties of AlSiC contrasted to traditional heat-sinking materials. AlSiC fabrication processing flow will also be reviewed and discussed. Functional packaging designs and application solutions will be illustrated through CPS AlSiC product examples.
The Grignard Reaction in the Synthesis of Ketones. I. A New Method of Preparing Desoxybenzoins (1933)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Grignard Reaction in the Synthesis of Ketones. I. A New Method of Preparing DesoxybenzoinsSanford S. JenkinsCite this: J. Am. Chem. Soc. 1933, 55, 2, 703–706Publication Date (Print):February 1, 1933Publication History Published online1 May 2002Published inissue 1 February 1933https://pubs.acs.org/doi/10.1021/ja01329a038https://doi.org/10.1021/ja01329a038research-articleACS PublicationsRequest reuse permissionsArticle Views196Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Carbon-Hydrogen Determination by Gas Chromatography (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbon-Hydrogen Determination by Gas ChromatographyA. A. Duswalt and W. W. BrandtCite this: Anal. Chem. 1960, 32, 2, 272–274Publication Date (Print):February 1, 1960Publication History Published online1 May 2002Published inissue 1 February 1960https://pubs.acs.org/doi/10.1021/ac60158a037https://doi.org/10.1021/ac60158a037research-articleACS PublicationsRequest reuse permissionsArticle Views233Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Progress of Researches on Transdiferentiation (2004)

Recent attention has focused on the remarkable ability of cells switching their phenotypes which iscalled transdifferentiation．States of developmental commitment are encoded as combination of transcription factors，andchanges in their expression could bring ab out transdifferentiation．Transdifferentiation wi ll have practical value in thedesign of novel therapies for the treatment of diseases when the new in vitro models of the process are set up．Recentlydiscovered cases of transdifferentiation were summarized，and the potential molecular and cellular mechan isms thatunderlie the switches，their significan ce of developmental biology and medicine were illumi nated．
An overview on the activation of reactivity of natural pozzolans (2001)

Natural pozzolan is one of the oldest construction materials. Lime-pozzolan cements have been used for several thousands of years and have an excellent reputation for their durability. Pozzolans are being widely used as a cement replacement in Portland cement concrete. The use of pozzolans has the advantage of lower costs and better durability, but the disadvantage of a longer setting time and a slower early strength development. Different techniques have been tried to increase the reactivity of natural pozzolans to overcome these disadvantages. This paper has reviewed various methods used to activate the pozzolanic activity of natural pozzolans. All activation methods can be classified into three catalogues: thermal, mechanical, and chemical activation. A comparison based on strengthcost relationship indicates that the chemical activation method is the most effective and cheapest one.Key words: activation, reactivity, natural pozzolans, pozzolanic reaction, calcination, elevated temperature curing, chemical activators, strength, cost.
High-precision flip-chip technology for alloptical wavelength conversion using SOI photonic circuit (2011)

High-precision hybrid integration on SOI photonic circuit was developed. The technology was applied to fabricate an integrated high-speed all-optical wavelength converter. Potential of the platform was demonstrated by 40Gb/s all-optical wavelength switching.
Lung defenses against Pseudomonas aeruginosa in C5-deficient mice with different genetic backgrounds (1986)

Lung defenses against Pseudomonas aeruginosa were investigated in C5-deficient strains of mice with different genetic backgrounds. We studied pulmonary clearance and cell responses after aerosol exposure to P. aeruginosa in C5-deficient B10.D2/oSnJ and DBA/2J mice and their closest C5-sufficient counterparts, B10.D2/nSnJ and DBA/1J mice. Different patterns of lung clearance and pulmonary cell responses were found for the two C5-deficient strains. C5-deficient B10.D2/oSnJ mice showed defective lung clearance of P. aeruginosa 4 h after challenge compared with C5-sufficient B10.D2/nSnJ animals. This finding was associated with a decreased number of polymorphonuclear leukocytes recruited into the airways during the same time. Interestingly, C5-deficient DBA/2J mice recruited higher numbers of polymorphonuclear leukocytes than did C5-sufficient DBA/1J mice by 4 h after aerosolization. Nevertheless, lung clearance of P. aeruginosa in DBA/2J mice was not as effective as in C5-sufficient DBA/1J mice, suggesting that other functions of C5 besides chemotaxism could be involved. Lung clearance of P. aeruginosa was also investigated in C5-deficient and -sufficient hybrids sharing the same genetic background (DBA/2J X B10.D2). The results suggested that murine lung clearance of P. aeruginosa is markedly affected by lack of C5 in a specific genetic background (B10.D2).
GAS TURBINE MIXED JET FLOWS BASED ON THE COANDĂ EFFECT (2015)

In this paper are presented some analytical results regarding the mixing jet flow devices used in the gas turbine engines. Recent achievements in advanced ejectors, nozzles and air flow rate amplifiers are presented. Two cases of specific flows ejectors were studied: ejector with normal ejection and uniform speeds; ejector with peripheral ejection and non-uniform speeds in admission region. The analysis is based on the theoretically and experimentally characteristics of a typical axial flow engine elements. The paper deals with the theoretical quantitative and qualitative aspects of the Coand phenomenon, in connection with the analytical Karman model.
Antibody Inhibitors to von Willebrand Factor Metalloproteinase and Increased Binding of von Willebrand Factor to Platelets in Ticlopidine-Associated Thrombotic Thrombocytopenic Purpura (2000)

In the patients who developed ticlopidine-associated TTP, autoantibodies to the vWF metalloproteinase were formed; this led to the same type of vWF abnormalities observed in patients with idiopathic acute TTP. The findings suggest that failure to process large and unusually large vWF multimers in vivo caused binding of vWF to platelets, systemic platelet thrombosis, and TTP.
Defined Anaerobic Growth Medium for Studying <i>Candida albicans</i> Basic Biology and Resistance to Eight Antifungal Drugs (2004)

The polymorphic fungus Candida albicans is one of the most versatile opportunistic pathogens in humans. Many organs of the human body are potential targets for infection by this pathogen, but infection is commonly localized in the gastrointestinal tract, an environment providing anaerobic growth conditions. We describe a chemically defined anaerobic growth medium for four strains of Candida albicans (A72, SC5314, MEN, and 10261). It is a defined liquid glucose-phosphate-proline growth medium supplemented with oleic acid, nicotinic acid, and ammonium chloride. The cells did not require or respond to added ergosterol. Oleic acid and nicotinic acid are growth factors which are required only for the anaerobic growth of C. albicans. An important technical feature of this study was the use of anaerobically grown inocula to study anaerobic growth. Anaerobically, the cells grew exclusively as mycelia at 25, 30, and 37 degrees C. The doubling time at 30 degrees C was ca. 20 h. The cells did not produce farnesol and did not respond to exogenous farnesol, and they were resistant to the highest tested levels of amphotericin B and four of the azole antifungals. We suggest that the anaerobic growth of C. albicans may contribute to the trailing end point phenomenon and the resistance of C. albicans biofilms to antifungal drugs.
The Effect of Leptin on Luteinizing Hormone Release Is Exerted in the Zona Incerta and Mediated by Melanin‐Concentrating Hormone (2000)

The adipose hormone, leptin, not only restrains appetite, but also influences energy expenditure. One such influence is to promote sexual maturation and fertility. The neuromodulatory circuits that mediate this effect are not well known but the present study suggests that one mediator could be melanin-concentrating hormone (MCH). We show that the long-form receptor (Ob-Rb) is expressed in the zona incerta of the rat and that administration of leptin (both 0.5 microg and 1.0 microg/side) into this area of ovariectomized, oestrogen-primed rats stimulated the release of luteinizing hormone (LH) within 1 h, the effect enduring for a further 1 h. Injections of leptin into the arcuate nucleus induced a smaller, transient rise in LH while injections into the paraventricular and ventromedial nuclei were without effect. MCH neurones are present in the zona incerta and administration of this hormone into the medial preoptic area (mPOA) stimulates LH release, therefore we investigated the possibility that MCH might mediate this effect of leptin. An injection of MCH antiserum into mPOA prevented the rise in LH normally induced by leptin injected into the zona incerta. In addition, melanocortin receptor antagonists ([D-Arg8]ACTH(4-10) and [Ala6]ACTH(4-10)), previously shown to inhibit the stimulatory effect of MCH on LH release, also inhibited the effect of leptin. We propose that one route by which leptin may promote reproductive activity is by enhancing MCH release from fibres within the mPOA. Speculative mechanisms for the action of MCH include the following possibilities: MCH may be acting on the specific MCH receptor which in turn interacts with a melanocortin or melanocortin-like receptor; MCH may bind directly to one of the melanocortin receptors; or melanocortin antagonists may interact with the MCH receptor.
Medial Spheres for Shape Approximation (2011)

We study the problem of approximating a 3D solid with a union of overlapping spheres. In comparison with a state-of-the-art approach, our method offers more than an order of magnitude speedup and achieves a tighter approximation in terms of volume difference with the original solid while using fewer spheres. The spheres generated by our method are internal and tangent to the solid's boundary, which permits an exact error analysis, fast updates under local feature size preserving deformation, and conservative dilation. We show that our dilated spheres offer superior time and error performance in approximate separation distance tests than the state-of-the-art method for sphere set approximation for the class of (σ,θ)-fat solids. We envision that our sphere-based approximation will also prove useful for a range of other applications, including shape matching and shape segmentation.
Lessons in <i>de novo</i> peptide sequencing by tandem mass spectrometry (2013)

Mass spectrometry has become the method of choice for the qualitative and quantitative characterization of protein mixtures isolated from all kinds of living organisms. The raw data in these studies are MS/MS spectra, usually of peptides produced by proteolytic digestion of a protein. These spectra are "translated" into peptide sequences, normally with the help of various search engines. Data acquisition and interpretation have both been automated, and most researchers look only at the summary of the identifications without ever viewing the underlying raw data used for assignments. Automated analysis of data is essential due to the volume produced. However, being familiar with the finer intricacies of peptide fragmentation processes, and experiencing the difficulties of manual data interpretation allow a researcher to be able to more critically evaluate key results, particularly because there are many known rules of peptide fragmentation that are not incorporated into search engine scoring. Since the most commonly used MS/MS activation method is collision-induced dissociation (CID), in this article we present a brief review of the history of peptide CID analysis. Next, we provide a detailed tutorial on how to determine peptide sequences from CID data. Although the focus of the tutorial is de novo sequencing, the lessons learned and resources supplied are useful for data interpretation in general.
Hematopoietic Engraftment and Survival in Adult Recipients of Umbilical-Cord Blood from Unrelated Donors (2001)

Umbilical-cord blood from unrelated donors can restore hematopoiesis in adults who receive myeloablative therapy and is associated with acceptable rates of severe acute and chronic GVHD.
A Small Molecule Inhibitor Selectively Induces Apoptosis in Cells Transformed by High Risk Human Papilloma Viruses (2016)

A phenotypic high-throughput cell culture screen was performed to identify compounds that prevented proliferation of the human Papilloma virus type 16 (HPV-16) transformed cell line Ca Ski. A series of quinoxaline compounds exemplified by Compound 1 was identified. Testing against a panel of cell lines demonstrated that Compound 1 selectively inhibited replication of all HPV-16, HPV-18, and HPV-31 transformed cell lines tested with 50% Inhibitory Concentration (IC50) values of 2 to 8 μM relative to IC50 values of 28 to 73 μM in HPV-negative cell lines. Treatment with Compound 1 resulted in a cascade of multiple apoptotic events, including selective activation of effector caspases 3 and 7, fragmentation of cellular DNA, and PARP (poly(ADP-ribose) polymerase) cleavage in HPV-positive cells relative to HPV-negative cells. Unregulated proliferation of HPV transformed cells is dependent on the viral oncogenes, E6 and E7. Treatment with Compound 1 resulted in a decrease in HPV E7 protein in Ca Ski cells. However, the timing of this reduction relative to other effects of compound treatment suggests that this was a consequence, rather than a cause, of the apoptotic cascade. Likewise, compound treatment resulted in no obvious effects on the E6- and E7- mediated down regulation of p53 and Rb, or their downstream effectors, p21 or PCNA. Further investigation of apoptotic signals induced by Compound 1 revealed cleavage of Caspase-8 in HPV-positive cells as early as 2 hours post-treatment, suggesting the compound initiates apoptosis through the extrinsic, death receptor-mediated, pathway of cell death. These studies provide proof of concept that cells transformed by oncogenic Papillomaviruses can be selectively induced to undergo apoptosis by compound treatment.
Effects of Aging on the Opioid Modulation of Feeding in Humans (2001)

We conclude that healthy older adults retain their sensitivity to the suppressive effects of naloxone on food intake. Possible gender differences in this sensitivity warrant further investigation. A decline in opioid activity is unlikely to contribute substantially to the physiological anorexia of aging observed in older people.