gebhart/patrepeval huggingface dataset:
CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection (2019)

CRISPR/Cas9 causes double-stranded DNA breaks that can undergo DNA repair either via non-homologous end joining (NHEJ) or, in the presence of a template, homology-directed repair (HDR). HDR is typically used to insert a specific genetic modification into the genome but has low efficiency compared to NHEJ, which is lowered even further when trying to create a homozygous change. In this study we devised a novel approach for homozygous single base editing based on utilising simultaneously two donor DNA templates cloned in plasmids with different antibiotic resistant genes. The donor templates were co-transfected alongside the CRISPR/Cas9 machinery into cells and a double antibiotic selection was optimised and allowed the isolation of viable desired clones. We applied the method for obtaining isogenic cells homozygous for variant B cystatin C, a recessive risk factor for age-related macular degeneration and Alzheimer's disease, in both induced Pluripotent Stem Cells (iPSCs) and a human RPE cell line. Bi-allelic gene edited clones were validated by sequencing, demonstrating that the double antibiotic templates approach worked efficiently for both iPSCs and human differentiated cells. We propose that this one step gene editing approach can be used to improve the specificity and frequency of introducing homozygous modifications in mammalian cells.
On profiling mobility and predicting locations of wireless users (2006)

In this paper, we analyze a year long wireless network users' mobility trace data collected on ETH Zurich campus. Unlike earlier work in [4,18], we profile the movement pattern of wireless users and predict their locations. More specifically, we show that each network user regularly visits a list of places such as a building (also referred to as "hubs") with some probability. The daily list of hubs, along with their corresponding visit probabilities, are referred to as a mobility profile. We also show that over a period of time (e.g., a week), a user may repeatedly follow a mixture of mobility profiles with certain probabilities associated with each of the profiles. Our analysis of the mobility trace data not only validate the existence of our so-called sociological orbits [8], but also demonstrate the advantages of exploiting it in performing hub-level location predictions In particular, we show that such profile based location predictions are more precise than common statistical approaches based on observed hub visitation frequencies alone.
<title>Dipyridophenazine complexes of Ru(II): versatile optical sensors for small and large molecules</title> (1997)

Bis(phenanthroline)dipyridophenazineruthenium(II), [(Ru(phen)<SUB>2</SUB>dppz]<SUP>2+</SUP>, exhibits unusual optical properties compared to other derivatives of [Ru(bpy)<SUB>3</SUB>]<SUP>2+</SUP>. [Ru(phen)<SUB>2</SUB>dppz]<SUP>2+</SUP> does not exhibit photoluminescence in water, but does emit brightly at approximately 610 nm in the presence of nonaqueous solvents or hydrated polymers like DNA and Nafion. We have observed this `light switch' effect upon binding [Ru(phen)<SUB>2</SUB>dppz]<SUP>2+</SUP> to a protein with a hydrophobic pocket (chymotrypsin), suggesting that we may be able to develop optical probes of protein tertiary structure. We have also prepared derivatives of [Ru(phen)<SUB>2</SUB>dppz]<SUP>2+</SUP> that have ancillary ligands other than phenanthroline, and find that these molecules change color upon exposure to small molecules; the environmental parameters we have been able to assess so far include Lewis acidity, the nature of hydrated counterions, polarity, and hydrogen-based donation ability.
Carrier capture and quantum confinement in GaAs/AlGaAs quantum wire lasers grown on V-grooved substrates (1992)

Carrier capture mechanisms in semiconductor quantum wire (QWR) lasers grown by organometallic chemical vapor deposition on nonplanar substrates were investigated by cathodoluminescence and photoluminescence (PL) spectroscopy. Efficient carrier capture into the QWRs via adjacent quantum wells is manifested by a complete transfer of luminescence intensity from the well- to the wire-spectral lines at temperatures above ∼100 K. In addition, higher QWR subbands separated by 19 meV are observed in the PL spectra, in agreement with the calculated subband spacing. The quantum well assisted carrier capture in these wires is important for the efficient room temperature operation of lasers and other optoelectronic devices based on QWRs.
The preparation and properties of O-methylated adenosine derivatives (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe preparation and properties of O-methylated adenosine derivativesJerry B. Gin and Charles A. DekkerCite this: Biochemistry 1968, 7, 4, 1413–1420Publication Date (Print):April 1, 1968Publication History Published online1 May 2002Published inissue 1 April 1968https://pubs.acs.org/doi/10.1021/bi00844a025https://doi.org/10.1021/bi00844a025research-articleACS PublicationsRequest reuse permissionsArticle Views139Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
PhoneEar (2015)

We present PhoneEar, a new approach that enables mobile devices to understand the broadcasted audio and sounds that we hear every day using existing infrastructure. PhoneEar audio streams are embedded with sound-encoded data using nearly inaudible high frequencies. Mobile devices then listen for messages in the sounds around us, taking actions to ensure we don't miss any important info. In this paper, we detail our implementation of PhoneEar, describe a study demonstrating that mobile devices can effectively receive sound-based data, and describe the results of a user study that shows that embedding data in sounds is not detrimental to sound quality. We also exemplify the space of new interactions, through four PhoneEar-enabled applications. Finally, we discuss the challenges to deploying apps that can hear and react to data in the sounds around us.
Minéralogie des Argiles (1964)

Keywords: Mineralogie ; Geologie ; Argile Reference Record created on 2004-09-07, modified on 2016-08-08
Fluorescent magnetic submicronic polymer (FMSP) nanoparticles induce cell death in human colorectal carcinoma cells (2018)

Nanoparticles have many advantages such as high biocompatibility, bioavailability and effective therapeutic capabilities. The aim of the present study is to examine whether fluorescent magnetic submicronic polymer nanoparticles (FMSP-nanoparticles) have any impact on human colorectal cancer cells. In the present study, we have tested FMSP-nanoparticles with an average size of 100-200 nm on human colorectal carcinoma cells (HCT-116) to check their cytotoxic and anti-cancer capabilities. The effects of FMSP-nanoparticles on cancer cells were observed after 6 h, 24 h and 48 h intervals. The response of FMSP-nanoparticles-treated cells was evaluated by Trypan Blue, 4lue-diamidino-2-phenylindole (DAPI) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Our MTT analysis results revealed that FMSP-nanoparticles produced dose-dependent effects on cancer cells, FMSP-nanoparticles with dose of 1.25 µg/mL did not decrease cell survivability, whereas dosages of 12.5 µg/mL and 50 µg/mL respectively showed 23.59% and 59.47% decrease in the cancer cell survivability. In conclusion, our results demonstrate FMSP-nanoparticles have a potential anti-cancer capability and hold a great promise for colon cancer treatments.
Optical Single Sideband (OSSB) Transmission for Dispersion Avoidance and Electrical Dispersion Compensation in Microwave Subcarrier and Baseband Digital Systems (1997)

In high frequency subcarrier systems chromatic dispersion gives rise to a relative delay or phase shift between the upper and low optical sidebands associated with the subcarrier frequency which upon square law detection results in a periodic variation of the received RF signal according to cos2(πDλ2f2L/c).
The Effects of Deoxyspergualin on the Development of Diabetes in Diabetes‐Prone BB Rats (1992)

The effects of the administration of the recently discovered immunosuppressant 15-Deoxyspergualin (DSP) on the development of insulin-dependent diabetes mellitus (IDDM) in diabetes-prone BB rats were studied. The data show that 2 mg/kg body weight DSP, administered six times a week from the 30th day up to the 105th day of age, significantly reduced the incidence of diabetes in diabetes-prone BB rats as compared with the PBS-injected controls. The drug was also able to reduce the signs of pancreatic insulitis and the percentages of W3/25+ and OX6+ splenocytes. Interruption of the treatment resulted in a later onset of diabetes in a high percentage of animals within 41 days. These findings suggest that 15-DSP may temporarily reverse the pathogenic mechanisms leading to beta cell destruction and autoimmune diabetes in a well-known experimental model of human insulin-dependent diabetes mellitus.
Quantification of the Steric Influence of Alkylphosphine–Sulfonate Ligands on Polymerization, Leading to High-Molecular-Weight Copolymers of Ethylene and Polar Monomers (2014)

A series of palladium/alkylphosphine-sulfonate catalysts were synthesized and examined in the homopolymerization of ethylene and the copolymerization of ethylene and polar monomers. Catalysts with alkylphosphine-sulfonate ligands containing sterically demanding alkyl substituents afforded (co)polymers whose molecular weight was increased by up to 2 orders of magnitude relative to polymers obtained from previously reported catalyst systems. The polymer molecular weight was found to be closely correlated to the Sterimol B5 parameter of the alkyl substituents in the alkylphosphine-sulfonate ligands. Thus, the use of bulky alkylphosphine-sulfonate ligands represents an effective and versatile method to prepare high-molecular-weight copolymers of ethylene and various polar monomers, which are difficult to obtain by previously reported methods.
High-Efficiency InGaN/GaN Dot-in-a-Wire Red Light-Emitting Diodes (2011)

We report on the achievement of high-performance InGaN/GaN dot-in-a-wire red light-emitting diodes on Si(111) substrates. Owing to the superior 3-D carrier confinement offered by the self-organized dot-in-a-wire heterostructures, the devices exhibit relatively high (~18%-32%) internal quantum efficiency at room temperature. Moreover, no efficiency droop was observed for injection current up to ~480A/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> under pulsed biasing conditions. We have also demonstrated that, by controlling the inhomogeneous broadening of the dot-in-a-wire heterostructures, the devices can exhibit relatively stable emission characteristics with increasing current.
Vanguard Management: An Emerging New Paradigm (1992)

There is an increasing body of evidence that significant and exciting changes are under way in how some organizations are seeing their world and transforming themselves to fit this new vision. The change is so fundamental as to constitute a paradigm shift. There is further evidence that some hospitality firms may be part of this transformation. The author advocates the use of vanguard management in this article
A common exocytotic mechanism mediates axonal and dendritic outgrowth. (2001)

Outgrowth of the dendrites and the axon is the basis of the establishment of the neuronal shape, and it requires addition of new membrane to both growing processes. It is not yet clear whether one or two exocytotic pathways are responsible for the respective outgrowth of axons and dendrites. We have previously shown that tetanus neurotoxin-insensitive vesicle-associated membrane protein (TI-VAMP) defines a novel network of tubulovesicular structures present both at the leading edge of elongating dendrites and axons of immature hippocampal neurons developing in primary culture and that TI-VAMP is an essential protein for neurite outgrowth in PC12 cells. Here we show that the expression of the N-terminal domain of TI-VAMP inhibits the outgrowth of both dendrites and axons in neurons in primary culture. This effect is more prominent at the earliest stages of the development of neurons in vitro. Expression of the N-terminal domain deleted form of TI-VAMP has the opposite effect. This constitutively active form of TI-VAMP localizes as the endogenous protein, particularly concentrating at the leading edge of growing axons. Our results suggest that a common exocytotic mechanism that relies on TI-VAMP mediates both axonal and dendritic outgrowth in developing neurons.
Agmatine reverses pain induced by inflammation, neuropathy, and spinal cord injury (2000)

Antagonists of glutamate receptors of the N-methyl-d-aspartate subclass (NMDAR) or inhibitors of nitric oxide synthase (NOS) prevent nervous system plasticity. Inflammatory and neuropathic pain rely on plasticity, presenting a clinical opportunity for the use of NMDAR antagonists and NOS inhibitors in chronic pain. Agmatine (AG), an endogenous neuromodulator present in brain and spinal cord, has both NMDAR antagonist and NOS inhibitor activities. We report here that AG, exogenously administered to rodents, decreased hyperalgesia accompanying inflammation, normalized the mechanical hypersensitivity (allodynia/hyperalgesia) produced by chemical or mechanical nerve injury, and reduced autotomy-like behavior and lesion size after excitotoxic spinal cord injury. AG produced these effects in the absence of antinociceptive effects in acute pain tests. Endogenous AG also was detected in rodent lumbosacral spinal cord in concentrations similar to those previously detected in brain. The evidence suggests a unique antiplasticity and neuroprotective role for AG in processes underlying persistent pain and neuronal injury.
Electronic structure and thermodynamic stability of<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mtext>LaMnO</mml:mtext></mml:mrow><mml:mn>3</mml:mn></mml:msub></mml:mrow></mml:math>and<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mtext>La</mml:mtext></mml:mrow><mml:mrow><mml:mn>1</mml:mn><mml:mo>−</mml:mo><mml:mi>x</mml:mi></mml:mrow></mml:msub><mml:msub><mml:mrow><mml:mtext>Sr</mml:mtext></mml:mrow><mml:mi>x</mml:mi></mml:msub><mml:msub><mml:mrow><mml:mtext>MnO</mml:mtext></mml:mrow><mml:mn>3</mml:mn></mml:msub></mml:mrow></mml:math>(001) surfaces:<i>Ab initio</i>calculations (2008)

We present the results of ab initio hybrid density-functional calculations of the atomic and the electronic structures of ${\text{LaMnO}}_{3}$ (LMO) and ${\text{La}}_{1\ensuremath{-}{x}_{b}}{\text{Sr}}_{{x}_{b}}{\text{MnO}}_{3}$ (001) surfaces. The total energies obtained from these calculations were used to analyze thermodynamic stability of the surfaces. We predict Sr and O vacancy segregation to the surface to occur with similar energies ($\ensuremath{\sim}0.5\text{ }\text{eV}$ per defect). In pure LMO only ${\text{MnO}}_{2}$ termination is thermodynamically favorable under typical operational conditions of a cathode in solid oxide fuel cells, whereas Sr doping makes La(Sr)O termination favorable. Finally, the role of Sr doping in cathode degradation is discussed.
The institutionalization of public hygiene in Korea, 1876-1910. (1995)

On the whole, the major impetus for the institutionalization of public hygiene in Korea came from two directions. On one hand, the self-enlightened intellectuals had introduced a variety of Western ideas and theories on public hygiene since the mid-eighteenth century. On the other hand, Japan strongly influenced the modern systems of Korean health care and medical education, especially through Japanese efforts at the sanitary control of infectious diseses such as smallpox and cholera. The institutionalization of Korea's public hygiene in this period corresponded not to the high ideas of the progressive intellectuals but to the larger social and institutional changes caused by the major political events. Ideas of public hygiene were institutionalized as a powerful strategy of linking the imperial capital and colonial domains.
An On-Line Spectrofluorimeter System for Rapid Collection of Absolute Luminescence Spectra (1971)

A spectrofluorimeter system has been developed which uses an on-line general purpose digital computer to both collect and correct fluorescence and luminescence spectra; no servosystems or complex analog circuitry are employed. Spectra are represented by a series of up to 500 individual data points, each of which is, in turn, the average of 16 samplings of the signal photomultiplier. The signal produced by a reference photomultiplier is also collected and used to make corrections appropriate to the type of spectra being collected. Wavelength drive is accomplished with a reversible stepping motor driven by commands from the computer; thus the wavelength position can be automatically and precisely controlled by the computer without recourse to cumbersome mechanical components. Corrections to collected spectra are made in real time on the basis of correction factors derived from fluorescence standards or a standard lamp. The complete system can collect a full spectrum (500 points) in 8 sec, average the results of any number of automatically repeated scans, and furnish the operator with both corrected and uncorrected spectra on either a wavelength or a wavenumber scale. In addition, the data can be retained on punched paper tape or presented graphically in a format acceptable for publication. Details of the system are presented with examples of its performance.
Tomato powdery mildew (1988)

A powdery mildew ( Erysiphe sp.) occurred on greenhouse‐grown tomato crops in southern England in 1987. White pustules appeared on the upper surfaces of leaves of affected crops and only rarely on the under surfaces. In greenhouse experiments, stems were very severely affected. In host range studies 19 tomato cultivars and six breeders' lines were susceptible as well as Nicotiana tabacum. N. xanthi. Solanium melongena, S. pseudocapsicum, S. tuberosum, Datura stramonium and Petunia hybrida. Conidia germinated readily at 15, 20 and 25 C and were viable for 2 days in the laboratory and at least 6 days in the greenhouse. The disease was well controlled by a range of fungicides including benomyl, bupirimate, carbendazim, chlorothalonil, fenarimol and pyrazophos. All of these except pyrazophos are approved for use on tomatoes in the UK. The pathogen was very sensitive to low concentrations of benomyl and fenarimol but less so to bupirimate.
TTSF: A Novel Two-Tier Spam Filter (2006)

Spam prevention is a classic puzzle in the research area of network security. The conventional spam filtering techniques still result to high false positives and have weak on-line processing ability. This paper presents a novel two-tier spam filter (TTSF) on the basis of analyses over the conventional anti-spam techniques. TTSF on-line filters spam by using URLs and off-line filters spam using digest-based approach. Experimental evaluations from the constructed prototype based on TTSF demonstrate it can significantly raise the filtering accuracy, effectively reduce false positives and can be applied to on-line processing and heavy traffic environment by reducing the computational cost than the state-of-the-art techniques
Dihydrochalcones from Piper longicaudatum (2001)

Bioactivity-guided fractionation of an ethanolic extract of the leaves and twigs of Piper longicaudatum Trelease & Yunker (Piperaceae) resulted in the isolation of one new (1) and three known (2-4) dihydrochalcones. The known compounds are: 2',6'-dihydroxy-4'-methoxydihydrochalcone (2), 2',6',4-trihydroxy-4'-methoxydihydrochalcone (asebogenin) (3), and 2'-hydroxy-4'-methoxy-2'-[1-hydroxy-1-methylethyl]-2",3"-dihy- drofurano[4",5":5',6"]-3"-[2-hydroxy-5-methoxycarbonylphe- nyl]dihydrochalcone (piperaduncin B) (4). The new compound is 2'-hydroxy-4'-methoxy-2"-[2-hydroxy-5-methoxycarbonyl- phenyl]-furano[4",5":5',6']-dihydrochalcone (longicaudatin) (1). Compounds 1-4 were tested for antibacterial activity against S. aureus and methicillin-resistant S. aureus (MRSA); only compound 3 showed inhibitory activity (IC50 of 10 and 4.5 micrograms/ml, respectively).
Effect of placement of occlusive membranes on root resorption and bone regeneration during healing of circumferential periodontal defects in dogs (1989)

Abstract Previous studies indicate that root resorption is a frequent sequela to significant amounts of periodontal repair in animal models. A model was developed in tabrador dogs to study periodontal wound healing in large circumferential defects in the absence of influence from the oral environment. A polytetrafluore‐thylene membrane of 1 μm pore size (GORE‐TEX®) was used to occlude cells from specific tissues from populating the healing den tin‐connective tissue wound. 3 modalities were studied: (1) control, in which no membrane and therefore no cell occlusion was used, (2) flap occlusion, in which the internal surface of the flap was lined with the membrane preventing contribution of cells from the flap, (3) bone occlusion, in which the reduced alveolar bone was lined with the membrane preventing contribution from bone cells. Wound closure included total submergence of teeth and membranes. The membranes remained incorporated and no exposure of membranes or teeth occurred during the 3 months healing period. Histology revealed bone regrowth in all 3 treatment modalities. It is suggested that inductive elements in the connective tissue or dentin, or bone cells from periosteum were responsible for bone regrowth in the bone membrane treatment modality. No significant difference was seen for root resorption between the 3 treatment modalities. This study found that physical occlusion of cells from the inside of the surgical flap, or from the pre‐existing bone, is not sufficient to prevent root resorption in periodontal wound healing.