gebhart/patrepeval huggingface dataset:
The course and prerequisites of Lys-plasminogen formation during fibrinolysis (1988)

Plasmin-catalyzed modification of the native plasma zymogen Glu1-plasminogen to its more reactive Lys78 form has been shown to be enhanced in the presence of fibrin. The aim of the present work has been to characterize the influence of fibrinopeptide release, fibrin polymerization, and plasmin cleavage of fibrin on the rate of Lys78-plasminogen formation. 125I-Labeled Glu1- to Lys78-plasminogen conversion was catalyzed by performed Lys78-plasmin, or by plasmin generated during plasminogen activation with tissue plasminogen activator or urokinase. The two forms of plasminogen were quantitated following separation by polyacrylamide gel electrophoresis in acetic acid/urea. Plasmin generated by plasminogen activator was monitored by a fixed-time amidolytic assay. The rate of Lys78-plasminogen formation was correlated, in separate experiments, to the simultaneous, plasmin-catalyzed cleavage of 125I-labeled fibrinogen or fibrin to fragments X, Y, and D. The radiolabeled components were quantitated after separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The results show that the formation of both bathroxobin-catalyzed des-A-fibrin and thrombin-catalyzed des-AB-fibrin leads to marked stimulation of Lys78-plasminogen formation, whereas inhibition of fibrin polymerization, with Gly-Pro-Arg-Pro, abolishes the stimulatory effect. The rate of Lys78-plasminogen formation varies markedly in the course of fibrinolysis. The apparent second-order rate constant of the reaction undergoes a transient increase upon transformation of fibrin to des-A(B) fragment X polymer and decreases about 10-fold to the level observed during fibrinogenolysis upon further degradation to soluble fragments Y and D.(ABSTRACT TRUNCATED AT 250 WORDS)
Operator-assisted harvesting of protein crystals using a universal micromanipulation robot (2007)

High-throughput crystallography has reached a level of automation where complete computer-assisted robotic crystallization pipelines are capable of cocktail preparation, crystallization plate setup, and inspection and interpretation of results. While mounting of crystal pins, data collection and structure solution are highly automated, crystal harvesting and cryocooling remain formidable challenges towards full automation. To address the final frontier in achieving fully automated high-throughput crystallography, the prototype of an anthropomorphic six-axis universal micromanipulation robot (UMR) has been designed and tested; this UMR is capable of operator-assisted harvesting and cryoquenching of protein crystals as small as 10 µm from a variety of 96-well plates. The UMR is equipped with a versatile tool exchanger providing full operational flexibility. Trypsin crystals harvested and cryoquenched using the UMR have yielded a 1.5 Å structure demonstrating the feasibility of robotic protein crystal harvesting.
Decreased Factor XIII Availability for Thrombin and Early Loss of Clot Firmness in Patients with Unexplained Intraoperative Bleeding (2004)

To explore relevant changes in unexplained intraoperative bleeding, we evaluated elements of the final steps of the coagulation cascade in 226 consecutive patients undergoing elective surgery. Patients were stratified for the occurrence of unexplained intraoperative bleeding according to predefined criteria. Twenty patients (8.8%) developed unexplained bleeding. The median intraoperative blood loss was 1350 mL (bleeders) and 400 mL (nonbleeders) (P < 0.001). Fibrinogen and Factor XIII (F. XIII) were more rapidly consumed in bleeders (P < 0.001). Soluble fibrin formation (fibrin monomer) was increased in bleeders throughout surgery (P < or = 0.014). However, F. XIII availability per unit thrombin generated was significantly decreased in bleeders before, during, and after surgery (P < or = 0.051). Computerized thrombelastography showed a parallel, significant reduction in clot firmness. We suggest that mild preexisting coagulopathy is not rare in surgical patients and probably can result in clinically relevant intraoperative bleeding. This hemostatic disorder shows impaired clot firmness, probably secondary to decreased cross-linking (due to a loss of F. XIII, both in absolute measures and per unit thrombin generated). We suggest that the application of F. XIII might be worthwhile to test in a prospective clinical trial to increase clot firmness in patients at risk for this intraoperative coagulopathy.
Proanthocyanidin Oligomers Isolated from <i>Salacia reticulata</i> leaves potently Inhibit Pancreatic Lipase Activity (2012)

Proanthocyanidin oligomers isolated from Salacia reticulata, referred to "Kothala himbutu" (KT) in Singhalese, leaves was proved to potently inhibit pancreatic lipase activity. After confirming in vivo examination, healthy foods, teas, and liquors containing the extracts of KT leaves are expected to be on market.
Effect of particle group parameters of GGBFS on its activity and blended cement properties (2010)

Based on filling effect and micro-aggregate effect of admixtures,the physical properties of cement prepared by GGBFS with different particle size distribution were tested,and the effect of slag particle group parameters on cement service performance and compressive strength ratio of blended cement was studied.The result showed that the wide distribution and coarse particle size of GGBFS was helpful to improve cement service performance.At the same time,the accumulation density of particles influenced the compressive strength ratio of blended cement especially at the early age,while the compressive strength ratio at later age was correlated to specific area of GGBFS.
1H-Cyclopenta[b]benzofuran Lignans from Aglaia Species Inhibit Cell Proliferation and Alter Cell Cycle Distribution in Human Monocytic Leukemia Cell Lines (1999)

Thirteen naturally occurring 1H-cyclopenta[b]benzofuran lignans of the rocaglamide type as well as one naturally occurring aglain congener all of them isolated from three Aglaia species (Aglaia duperreana, A. oligophylla and A. spectabilis) collected in Vietnam were studied for their antiproliferative effects using the human monocytic leukemia cell lines MONO-MAC-1 and MONO-MAC-6. Only rocaglamide type compounds showed significant inhibition of [3H-]thymidine incorporation and the most active compound didesmethylrocaglamide inhibited cell growth in a similar concentration range as the well-known anticancer drug vinblastine sulfate. Detailed structure-activity analysis indicated that the OH-group at C-8b which is a common structural feature of most naturally occurring rocaglamide compounds is essential for the described antiproliferative activity since replacement of this group by methylation led to a complete loss of the inhibitory activity for the resulting derivative. Rocaglamide derivatives rapidly inhibited DNA as well as protein biosynthesis of MONO-MAC-6 cells at concentrations well below those of actinomycin D or cycloheximide which were used as positive controls in the respective experiments. Didesmethylrocaglamide was furthermore able to induce growth arrest of MONO-MAC-1 cells in the G2/M and probably G0/G1-phase of the cell cycle with no morphological indication of cellular damage. Our data suggests that 1H-cyclopenta[b]benzofuran lignans of the rocaglamide type act primarily by a cytostatic mechanism.
An Active Vision System for Detecting, Fixating and Manipulating Objects in the Real World (2009)

The ability to autonomously acquire new knowledge through interaction with the environment is an important research topic in the field of robotics. The knowledge can only be acquired if suitable perception— action capabilities are present: a robotic system has to be able to detect, attend to and manipulate objects in its surrounding. In this paper, we present the results of our long-term work in the area of vision-based sensing and control. The work on finding, attending, recognizing and manipulating objects in domestic environments is studied. We present a stereo-based vision system framework where aspects of top-down and bottom-up attention as well as foveated attention are put into focus and demonstrate how the system can be utilized for robotic object grasping.
Hot Dry Rock geothermal energy moving towards practical applications (1994)

The thermal energy present in hot rock at depth is a vast resource which has so far been tapped only in those unusual locations where natural fluids exist to transport that energy to the surface. For the past twenty years work has been underway at the Los Alamos National Laboratory to develop the technology to access and recovery the heat present in rock which is hot but contains no natural mobile fluid. The world`s first plant capable of sustained production of geothermal energy from HDR was completed in 1991. This facility combined an artificial geothermal reservoir of sufficient size and high enough temperature to deliver large amounts of useful energy with a surface plant built to power industry standards and capable of sustained, routine operation. During the past two years, extended testing at Fenton Hill has demonstrated that energy can be extracted from HDR on a continuous basis. Thermal energy was produced continuously at a rate of about 4 MW in two test phases lasting 112 and 55 days, respectively, and intermittently for a period of 7 1/2 months between the continuous test segments. Temperature measurements at the surface and at depth indicated no decline in the average discharge temperature of water from the reservoir over the span of the test. In fact, tracer testing indicated that access of the circulating water to the hot reservoir rock improved as the test proceeded.
Improved signal processing to detect cancer by ultrasonic molecular imaging of targeted nanoparticles (2011)

In several investigations of molecular imaging of angiogenic neovasculature using a targeted contrast agent, Renyi entropy [I(f)(r)] and a limiting form of Renyi entropy (I(f,∞)) exhibited significantly more sensitivity to subtle changes in scattering architecture than energy-based methods. Many of these studies required the fitting of a cubic spline to backscattered waveforms prior to calculation of entropy [either I(f)(r) or I(f,∞)]. In this study, it is shown that the robustness of I(f,∞) may be improved by using a smoothing spline. Results are presented showing the impact of different smoothing parameters. In addition, if smoothing is preceded by low-pass filtering of the waveforms, further improvements may be obtained.
Distributed fault detection of wireless sensor networks (2006)

Wireless Sensor Networks (WSNs) have become a new information collection and monitoring solution for a variety of applications. Faults occurring to sensor nodes are common due to the sensor device itself and the harsh environment where the sensor nodes are deployed. In order to ensure the network quality of service it is necessary for the WSN to be able to detect the faults and take actions to avoid further degradation of the service. The goal of this paper is to locate the faulty sensors in the wireless sensor networks. We propose and evaluate a localized fault detection algorithm to identify the faulty sensors. The implementation complexity of the algorithm is low and the probability of correct diagnosis is very high even in the existence of large fault sets. Simulation results show the algorithm can clearly identify the faulty sensors with high accuracy.
Basic Considerations for Fluid Anomaly Seismic Signal Detection, and Mapping Reservoir (1993)

The detection and structural confirmation of the fluid anomaly signal in the context of the stratal geometry, with stratal attribute displays overlain on depth contour maps, should generally provide for a low detection threshold and a low false alarm rate on modem 3D data, with major improvements in data quality hoped for in the future.
Effects of propafenone and 5‐hydroxy‐propafenone on hKv1.5 channels (1998)

1. The goal of this study was to analyse the effects of propafenone and its major metabolite, 5-hydroxy-propafenone, on a human cardiac K+ channel (hKv1.5) stably expressed in Ltk- cells and using the whole-cell configuration of the patch-clamp technique. 2. Propafenone and 5-hydroxy-propafenone inhibited in a concentration-dependent manner the hKv1.5 current with K(D) values of 4.4+/-0.3 microM and 9.2+/-1.6 microM, respectively. 3. Block induced by both drugs was voltage-dependent consistent with a value of electrical distance (referenced to the cytoplasmic side) of 0.17+/-0.55 (n=10) and 0.16+/-0.81 (n=16). 4. The apparent association (k) and dissociation (l) rate constants for propafenone were (8.9+/-0.9) x 10(6) M(-1) s(-1) and 39.5+/-4.2 s(-1), respectively. For 5-hydroxy-propafenone these values averaged (2.3+/-0.3) x 10(6) M(-1) s(-1) and 21.4+/-3.1 s(-1), respectively. 5. Both drugs reduced the tail current amplitude recorded at -40 mV after 250 ms depolarizing pulses to +60 mV, and slowed the deactivation time course resulting in a 'crossover' phenomenon when the tail currents recorded under control conditions and in the presence of each drug were superimposed. 6. Both compounds induced a small but statistically significant use-dependent block when trains of depolarizations at frequencies between 0.5 and 3 Hz were applied. 7. These results indicate that propafenone and its metabolite block hKv1.5 channels in a concentration-, voltage-, time- and use-dependent manner and the concentrations needed to observe these effects are in the therapeutical range.
In-line Gas Analyzer for UF6 and F2 through Differential Thermal Conductivity Measurements (1973)

For the purpose of analyzing UF6 and F2 continuously in the fluorination of U, a system of differential thermal conductivity cells (TCC), equipped with shock absorber, and also NaF and KCl traps, has been trially constructed. Mixed flow-through/diffusion type katharometers are used, provided with four and two Ni filaments (∼30Ω) on the UF6 and F2 cells, respectively. Based on data obtained from preliminary operating tests, the standard conditions for operating the system were established i.e., 500 mmHg abs. Cell pressure, 200 ml/min flow rate and 80 mA bridge current. Under these operating conditions and for UF6 and F2 concentrations of 2 and 20 v/o respectively, the overall out-put variations are below 1%. The total response time of the system including the transport lag is below 1 and 2.5 min for UF6 and F2 cells, respectively. The dead time due to blow-back noise is observed to have about the same period as the above response time. The sensitivities for UF6 and F2 are found respectively to be 11.5 and 0.37 mV/v/o, for which the limits of analytical determination are 0.01 and 0.05 v/o;, respectively. By practical application to uranium fluorination, the TCC system thus devised, has proven to possess the requisite characteristics for measurements of reaction rate, fluorine utilization and end of reaction in fluid-bed fluorination of uranium oxides. The system has possibilities of application to the study of reaction steps.
The Lack of Efficacy of 4,6,4′‐Trimethylangelicin to Induce Immune Suppression in an Animal Model for Photopheresis: a Comparison with 8‐MOP (1996)

Photopheresis is an extracorporeal form of photochemotherapy with 8-methoxypsoralen (8-MOP) and UVA (PUVA). Patients ingest 8-MOP and then a psoralen-rich buffy coat is obtained by centrifugation and mixed with saline. This mixture is recirculated through a UVA radiation field and then reinfused. Photopheresis appears to be effective for several T cell-mediated disorders, because the treatment results in a specific immune response against the pathogenic clone of T cells involved. With PUVA therapy, the whole body of the patient is exposed to UVA, after ingestion of 8-MOP. Upon UVA exposure 8-MOP binds to, amongst others, DNA and induces DNA monoadducts and interstrand cross-links. As a result of these photoadducts photocarcinogenicity is a risk in PUVA. In PUVA for psoriasis, it proved that angular furocoumarins, although almost incapable of inducing DNA cross-links (less carcinogenic), are still effective. In order to determine if monoadducts induced by photopheresis could also be effective we used, specifically, 4,6,4'-trimethylangelicin (TMA). In this report, we compare the photodegradation of both TMA and 8-MOP under conditions relevant to the in vivo situation, as well as the effect both compounds have on the viability of rat lymphocytes as measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. We show that TMA did not induce immunosuppression in vivo, even after extensive irradiation. In addition a dose dependency of 8-MOP/UVA versus the induced immune suppression was carried out. It was shown that there is a log dose/response correlation of r=0.9205.
Serendipity and the Discovery of Novel Compounds That Restore Mitochondrial Plasticity (2014)

The mitochondrial electron transport chain (ETC) plays a central role in energy generation in the cell. Mitochondrial dysfunctions diminish adenosine triphosphate (ATP) production and result in insufficient energy to maintain cell function. As energy output declines, the most energetic tissues are preferentially affected. To satisfy cellular energy demands, the mitochondrial ETC needs to be able to elevate its capacity to produce ATP at times of increased metabolic demand or decreased fuel supply. This mitochondrial plasticity is reduced in many age-associated diseases. In this review, we describe the serendipitous discovery of a novel class of compounds that selectively target cardiolipin on the inner mitochondrial membrane to optimize efficiency of the ETC and thereby restore cellular bioenergetics in aging and diverse disease models, without any effect on the normal healthy organism. The first of these compounds, SS-31, is currently in multiple clinical trials.
Purification and Characterization of a Cytochrome P450 (<italic>trans</italic>-Cinnamic Acid 4-Hydroxylase) from Etiolated Mung Bean Seedlings (1993)

A Cyt P450 (P450C4H) possessing trans-cinnamate 4-hydroxylase (C4H) activity was purified to apparent homogeneity from microsomes of etiolated mung bean seedlings. Upon SDS-polyacrylamide gel electrophoresis, the purified preparation gave a single protein band with a molecular mass of 58-kDa. Its specific P450 content was 12.6 nmol (mg protein)−1. Using NADPH as electron donor, purified P450C4H aerobically converted trans-cinnamic acid to p-coumaric acid with a specific activity of 68 nmol min−1 nmol−1 P450 in a reconstituted system containing NADPH-Cyt P450 reductase purified from the seedlings or rabbit liver microsomes, dilauroyl phosphatidylcholine, and cholate. This specific activity is by far the highest for reconstituted C4H systems so far reported and provides direct evidence that C4H activity is actually associated with a P450 protein. In the oxidized state P450C4H showed a typical low-spin type absorption spectrum with a Soret peak at 419 nm. A partial spectral shift to the high spin state was observed when trans-cinnamic acid was added to oxidized P450C4H. By spectral titration, the dissociation constant of the cinnamic acid-P450C4H complex was determined to be 2.8 μM. This value is similar to the Km value (1.8 μM) for trans-cinnamic acid determined in the reconstituted system.
Truncated RON Tyrosine Kinase Drives Tumor Cell Progression and Abrogates Cell-Cell Adhesion Through E-Cadherin Transcriptional Repression (2004)

RON is a tyrosine kinase receptor that triggers scattering of normal cells and invasive growth of cancer cells on ligand binding. We identified a short RON mRNA, which is expressed in human lung, ovary, tissues of the gastrointestinal tract, and also in several human cancers, including ovarian carcinomas and cell lines from pancreatic carcinomas and leukemias. This transcript encodes a truncated protein (short-form RON; sf-RON), lacking most of the RON receptor extracellular domain but retaining the whole transmembrane and intracellular domains. Sf-RON shows strong intrinsic tyrosine kinase activity and is constitutively phosphorylated. Epithelial cells transduced with sf-RON display an aggressive phenotype; they shift to a nonepithelial morphology, are unable to form aggregates, grow faster in monolayer cultures, show anchorage-independent growth, and become motile. We show that in these cells, E-cadherin expression is lost through a dominant transcriptional repression pathway likely mediated by the transcriptional factor SLUG. Altogether, these data show that expression of a naturally occurring, constitutively active truncated RON kinase results in loss of epithelial phenotype and aggressive behavior and, thus, it might contribute to tumor progression.
Microstructure and Mechanical Properties of SiC Platelet/Cordierite Glass‐Ceramic Composites (1995)

Cordierite glass‐ceramics reinforced with different volume fractions (0, 10, 20, and 30%) of SiC platelets were fabricated by mechanically mixing appropriate powders and hot pressing above the glass transition temperature. Some of the specimens were subjected to postsintering crystallization treatments. The resultant sintered microstructure was textured, and within it the SiC platelets tended to be oriented with their basal planes perpendicular to the hot‐pressing direction. The bending strength of the hot‐pressed specimens was constant, while the fracture toughness increased with the SiC content. Crystallization heat treatment in argon for 30 min at 1200°C caused a significant decrease in the bending strength, but improved the fracture toughness. Fractography has confirmed the crack deflection to be a dominant mechanism in the crystallized specimens, which contributes to the improvement in the fracture toughness. The overall changes in the mechanical properties are discussed with respect to the change in the residual stresses and oxidation characteristics of the SiC platelets.
SILICON MOLECULAR BEAM EPITAXY : STATUS ; DEVICES ; TRENDS (1988)

The single crystal growth, by molecular beam epitaxy, of materials compatible with silicon is described, and device applications are given. Process temperatures are decreased to 240°C, heterojunction devices are fabricated, strained layer Si/Ge superlattices are investigated, and silicon based monolithic integration of heterojunction devices and conventional devices is suggested.
S052014 Efficient Decomposition of Perfluoro Compounds Using Plasma Generated Inside Gas Bubbles Due to Adsorption (2011)

Perfluorooctane sulfonic acid (PFOS) is environmentally harmful and persistent substance. The decomposition of PFOS in water using plasma inside oxygen bubbles has been successfully conducted and the energy efficiency is relatively high compared to other methods such as photochemical process. The amount of PFOS adsorbing to the plasma?water interface showed much higher mole fraction of PFOS (6.0 X 10^-4) than that in bulk water (1.8 X 10^-6) at PFOS bulk concentration of 50 mg/L. Thus it is concluded that the plasma could effectively react with PFOS on the water surface and showed high energy efficiency.
Some Variation in Estrogenic Activity in Fresh and Dried White Clover Clones and the Ladino Variety (1960)

Journal Article Some Variation in Estrogenic Activity in Fresh and Dried White Clover Clones and the Ladino Variety Get access E. M. Bickoff, E. M. Bickoff Search for other works by this author on: Oxford Academic PubMed Google Scholar A. L. Livingston, A. L. Livingston Search for other works by this author on: Oxford Academic PubMed Google Scholar A. N. Booth, A. N. Booth Search for other works by this author on: Oxford Academic PubMed Google Scholar C. R. Thompson, C. R. Thompson Search for other works by this author on: Oxford Academic PubMed Google Scholar E. A. Hollowell, E. A. Hollowell Search for other works by this author on: Oxford Academic PubMed Google Scholar E. G. Beinhart E. G. Beinhart Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Animal Science, Volume 19, Issue 4, November 1960, Pages 1143–1149, https://doi.org/10.2527/jas1960.1941143x Published: 01 November 1960