gebhart/patrepeval huggingface dataset:
Review article: Luminex technology for HLA antibody detection in organ transplantation (2009)

Since its inception in the early 1960s, the serologically based complement-dependent cytotoxicity (CDC) assay has been the cornerstone technique for the detection of human leucocyte antigen (HLA) antibodies, not only in pre-transplant renal patients, but also in other forms of organ transplantation. Recently, solid phase assays have been developed and introduced for this purpose, and in particular the Flow-based bead assays such as the Luminex system. This latter assay has proved to be far more sensitive than the CDC assay and has revealed pre-sensitization in potential transplant recipients not detected by other methods of HLA antibody detection. However, the clinical implications of this increased sensitivity have not been convincingly demonstrated until recently. This technology for HLA antibody detection permits the evaluation of the clinical importance of antibodies directed at, for example, HLA-DPB1 and HLA-DQA1, which has not been possible to date. There are Luminex issues, however, requiring resolution such as the ability to distinguish between complement fixing and non-complement fixing antibodies and determination of their relative clinical significance. Luminex technology will permit a re-evaluation of the role of HLA antibodies in both early and late antibody-mediated rejection.
A Summary of the New York Urban Air Pollution Dynamics Research Program (1967)

A description is given of the experimental techniques employed in a study of urban air pollution in the New York Metropolitan Area. The experimental measurements consist of an anemometer network, an SO2 ground network, an upper level wind network and programmed flight of a helicopter, and fixed winged aircraft equipped to measure pressure height, temperature, wet bulb depression, and SO2 concentration. Some highlights of the observational program are shown. These include detailed pictures of the height distribution of the urban heat island, associated wind flow patterns, and their effect on the distribution of concentration within the city. Some examples are given of the three-dimensional distribution of temperature and SO2 within the urban area. These observations are discussed in the context of a numerical model.
Investigation of a periodically segmented waveguide Fabry-Pe/spl acute/rot interferometer for use as a chemical/biosensor (2006)

We present a periodically segmented waveguide Fabry-Pe/spl acute/rot interferometer (PSW-FPI) intended to be used for tagless real-time chemical/ biological sensing through bulk-material interaction. The differential sensor detects changes in the refractive index (RI) of a sample regardless of its absolute RI value. Experiments with a series of sucrose solutions of various concentrations are compared with theoretical results, and a very good match is found between the two. The theoretical sensitivity limit for a 50-dB-signal-to-noise-ratio (SNR) measurement system is estimated as /spl delta/n=3/spl middot/10/sup -7/. For a 29-dB-SNR measurement system, a measured sensitivity limit of /spl delta/n=4/spl middot/10/sup -5/ is comparable with the previously reported sensitivities of label-free real-time optical biosensors (2/spl middot/10/sup -5/-5/spl middot/10/sup -5/). However, the total required sensing length (720 /spl mu/m) of our PSW-FPI sensor is much shorter than that of the previously reported devices (9-20 mm).
Overexpression of E-cadherin on Melanoma Cells Inhibits Chemokine-promoted Invasion Involving p190RhoGAP/p120ctn-dependent Inactivation of RhoA (2009)

Melanoma cells express the chemokine receptor CXCR4 that confers high invasiveness upon binding to its ligand CXCL12. Melanoma cells at initial stages of the disease show reduction or loss of E-cadherin expression, but recovery of its expression is frequently found at advanced phases. We overexpressed E-cadherin in the highly invasive BRO lung metastatic cell melanoma cell line to investigate whether it could influence CXCL12-promoted cell invasion. Overexpression of E-cadherin led to defective invasion of melanoma cells across Matrigel and type I collagen in response to CXCL12. A decrease in individual cell migration directionality toward the chemokine and reduced adhesion accounted for the impaired invasion. A p190RhoGAP-dependent inhibition of RhoA activation was responsible for the impairment in chemokine-stimulated E-cadherin melanoma transfectant invasion. Furthermore, we show that p190RhoGAP and p120ctn associated predominantly on the plasma membrane of cells overexpressing E-cadherin, and that E-cadherin-bound p120ctn contributed to RhoA inactivation by favoring p190RhoGAP-RhoA association. These results suggest that melanoma cells at advanced stages of the disease could have reduced metastatic potency in response to chemotactic stimuli compared with cells lacking E-cadherin, and the results indicate that p190RhoGAP is a central molecule controlling melanoma cell invasion.
Sam68 RNA Binding Protein Is an In Vivo Substrate for Protein Arginine <i>N</i> -Methyltransferase 1 (2003)

RNA binding proteins often contain multiple arginine glycine repeats, a sequence that is frequently methylated by protein arginine methyltransferases. The role of this posttranslational modification in the life cycle of RNA binding proteins is not well understood. Herein, we report that Sam68, a heteronuclear ribonucleoprotein K homology domain containing RNA binding protein, associates with and is methylated in vivo by the protein arginine N-methyltransferase 1 (PRMT1). Sam68 contains asymmetrical dimethylarginines near its proline motif P3 as assessed by using a novel asymmetrical dimethylarginine-specific antibody and mass spectrometry. Deletion of the methylation sites and the use of methylase inhibitors resulted in Sam68 accumulation in the cytoplasm. Sam68 was also detected in the cytoplasm of PRMT1-deficient embryonic stem cells. Although the cellular function of Sam68 is unknown, it has been shown to export unspliced human immunodeficiency virus RNAs. Cells treated with methylase inhibitors prevented the ability of Sam68 to export unspliced human immunodeficiency virus RNAs. Other K homology domain RNA binding proteins, including SLM-1, SLM-2, QKI-5, GRP33, and heteronuclear ribonucleoprotein K were also methylated in vivo. These findings demonstrate that RNA binding proteins are in vivo substrates for PRMT1, and their methylation is essential for their proper localization and function.
Glucagon-like Peptide-1 Analogue and Dipeptidyl Peptidase-IV Inhibitors (2006)

□ 지상강좌
Forward-tracking: a technique for searching beyond failure (2005)

In many applications, such as decision support, negotiation, planning, scheduling, etc., one needs to express requirements that can only be partially satisfied. In order to express such requirements, we propose a technique called forward-tracking. Intuitively, forward-tracking is a kind of dual of chronological back-tracking: if a program globally fails to find a solution, then a new execution is started from a program point and a state 'forward' in the computation tree. This search technique is applied to constraint logic programming, obtaining a powerful extension that preserves all the useful properties of the original scheme. We report on the successful practical application of forward-tracking to the evolutionary training of(constrained) neural networks.
Effect of Conductive Additives on the Structural and Electrochemical Properties of Li<sub>4</sub>Ti<sub>5</sub>O<sub>12</sub>Spinel (2012)

The effect of a conductive agent on the structural and electrochemical properties of <TEX>$Li_4Ti_5O_{12}$</TEX>(LTO) spinel was investigated through neutron diffraction during Li intercalation and electrochemical measurements. The charging process of LTO is known as transformation of the white <TEX>$(Li_3)_{8a}[LiTi_5]_{16d}O_{12}$</TEX> into a dark-colored <TEX>$(Li_{3-X})_{8a}[Li_{X+Y}]_{16c}[LiTi_5]_{16d}O_{12}$</TEX> by incorporating the inserted Li into octahedral 16c sites, and the Li in tetrahedral 8a sites shifted to 16c sites. The occupancy of the tetrahedral 8a site varied with the existence of carbon in the electrode. Without carbon, the lattice parameter and cell volume of LTO decreased more notably than in the carbon-containing LTO electrode during Li insertion process. These phenomena might be attributed that the Li occupancy of the tetrahedral 8a of the LTO electrode without carbon was less than that of the carbon-containing LTO electrode.
The State of the Art of Clinical Hypnosis (1987)

Abstract This paper reviews the contemporary status of clinical hypnosis in light of the current emphasis on briefer, pragmatic forms of therapy and consumer demands to demonstrate effectiveness. Conceptual shifts and an expansion of clinical applications are related to changes in hypnotic strategies and technique. Suggestions for future avenues of clinical research are outlined and the importance of the continued integration of scientific rigor, empirical clarity, and clinical acumen and sensitivity is emphasized.
Sparsity Enhanced Decision Feedback Equalization (2012)

For single-carrier systems with frequency domain equalization, decision feedback equalization (DFE) performs better than linear equalization and has much lower computational complexity than sequence maximum likelihood detection. The main challenge in DFE is the feedback symbol selection rule. In this paper, we give a theoretical insight on multiple symbol selection, based on sparsity and we present a simple algorithm for DFE. The algorithm converges fast and has a low computational cost. We show how the initial solution can be obtained via convex relaxation instead of linear equalization, and illustrate the impact that the choice of the initial solution has on the bit error rate performance of our algorithm. The algorithm is applicable in several existing wireless communication systems (SC-FDMA, MC-CDMA, MIMO-OFDM). Numerical results illustrate significant performance improvement in terms of bit error rate compared to the MMSE solution.
Improved Outcome Based on Fluid Management in Critically III Patients Requiring Pulmonary Artery Catheterization (1992)

We performed a randomized, prospective trial to evaluate whether fluid management that emphasized diuresis and fluid restriction in patients with pulmonary edema could affect the development or resolution of extravascular lung water (EVLW), as well as time on mechanical ventilation and time in the intensive care unit (ICU), in critically ill patients requiring pulmonary artery catheterization (PAC). PAC was performed on 101 patients. A total of 52 patients were randomized to an EVLW management group using a protocol based on bedside indicator-dilution measurements of EVLW. The other 49 patients were randomized to a wedge pressure (WP) management group in whom fluid management decisions were guided by WP measurements. A total of 89 patients had pulmonary edema (defined as EVLW greater than 7 ml/kg ideal body weight). Except for a clinically unimportant difference in mean age, the two groups were entirely comparable at baseline. The study groups were managed differently, as evidenced by cumulative input-output of 2,239 +/- 3,695 ml (median = 1,600 ml) in the WP group versus 142 +/- 3,632 ml (median = 754 ml) in the EVLW group (p = 0.001). EVLW decreased significantly, and ventilator-days and ICU days were significantly shorter only in patients from the EVLW group. No clinically significant adverse effect occurred as a result of following the EVLW group algorithm. Thus, a lower positive fluid balance, especially in patients with pulmonary edema regardless of cause, is associated with reduced EVLW, ventilator-days, and ICU days.
Robot-based augmentative and alternative communication for nonverbal children with communication disorders (2014)

Nonverbal children with communication disorders have difficulties communicating through oral language. To facilitate communication, Augmentative and Alternative Communication (AAC) is commonly used in intervention settingss. Different forms of AAC have been used; however, one key aspect of AAC is that children have different preferences and needs in the intervention process. One particular AAC method does not necessarily work for all children. Although robots have been used in different applications, this is one of the first times that robots have been used for improvement of communication in nonverbal children. In this work, we explore robot-based AAC through humanoid robots that assist therapists in interventions with nonverbal children. Through playing activities, our study assessed changes in gestures, vocalization, speech, and verbal expression in children. Our initial results show that robot-based AAC intervention has a positive impact on the communication skills of nonverbal children.
Phonon Self-Energy Corrections to Nonzero Wave-Vector Phonon Modes in Single-Layer Graphene (2012)

Phonon self-energy corrections have mostly been studied theoretically and experimentally for phonon modes with zone-center (q=0) wave vectors. Here, gate-modulated Raman scattering is used to study phonons of a single layer of graphene originating from a double-resonant Raman process with q≠0. The observed phonon renormalization effects are different from what is observed for the zone-center q=0 case. To explain our experimental findings, we explored the phonon self-energy for the phonons with nonzero wave vectors (q≠0) in single-layer graphene in which the frequencies and decay widths are expected to behave oppositely to the behavior observed in the corresponding zone-center q=0 processes. Within this framework, we resolve the identification of the phonon modes contributing to the G(⋆) Raman feature at 2450 cm(-1) to include the iTO+LA combination modes with q≠0 and also the 2iTO overtone modes with q=0, showing both to be associated with wave vectors near the high symmetry point K in the Brillouin zone.
CD23 Sheddase A Disintegrin and Metalloproteinase 10 (ADAM10) Is Also Required for CD23 Sorting into B Cell-derived Exosomes (2010)

The low affinity receptor for IgE, CD23, is the natural regulator of IgE synthesis, and understanding both the synthesis and the catabolism of CD23 are, thus, important issues. Membrane CD23 is cleaved by a disintegrin and metalloproteinase 10 (ADAM10) and this cleavage influences the ability of CD23 to regulate IgE. In contrast to the belief that cleavage is a cell surface event, endosomal neutralization with NH(4)Cl was found to dramatically reduce CD23 cleavage, suggesting that the majority of CD23 cleavage occurred subsequent to internalization in the endosomal pathway and not at the cell surface. In line with this, full-length CD23 was shown to be sorted in an ADAM10-dependent manner into exosomes. Greatly increased ADAM10-mediated CD23 cleavage was seen at endosomal pH. Additionally, the stalk region of CD23 was found to interact with ADAM10 and ADAM10 binding of CD23 was found to be protease independent. SPR analysis of the interaction indicated about a 10-fold increase in the R(max) at endosomal pH (pH 5.8) compared with pH 7.4, whereas the affinity of the interaction was not significantly changed. The R(max) change, combined with the increased cleavage at endosomal pH, indicates greater accessibility of the CD23 stalk region for ADAM10 at the lower pH. These results indicate a model where CD23 internalization results in ADAM10-dependent incorporation into exosomes, followed by partial cleavage of CD23 by ADAM10 prior to being released from the cell. The increased cleavage at endosomal pH also has implications for other ADAM10 substrates.
Information type filters for tracking a maneuvering target (1990)

Two previously proposed adaptive covariance-type Kalman filtering techniques for tracking maneuvering targets (see Y.T. Chan et al. ibid., p.237-44, Mar. 1979, and Z. Tang et al. Report, Department of Electrical and Computer Engineering, Oregon State University, Corvallis, Oct. 1983) are developed further to utilize the information-type Kalman filter. These adaptive information-type filters are described in structurally decoupled forms, thereby greatly reducing the computational requirements while rendering the filters amenable to implementation on parallel processors. A coherent decision procedure for including partial coupling when necessary is developed via offline analysis of crosscorrelation functions.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Role of physical forces in compensatory growth of the lung (1989)

In many species, partial resection of the lung leads to rapid compensatory growth of the remaining tissue to restore normal lung mass and function. The response to partial pneumonectomy is closely controlled; both its rate and nature are subject to hormonal modulation. Physical factors, particularly distortion of the lung by altered inflation, are likely involved in regulation of the response, although the details of the regulatory mechanisms are not understood. In a number of tissues including the lung, application of external physical force leads to both acute and long-term changes in metabolism. In some cases these include cell growth and division, along with increased production of extracellular matrix components. Similar responses have been described after application of stress to isolated cells in culture. Independent lines of investigation have defined dramatic influences of cell shape on growth, differentiation, and metabolism, but stress-strain relationships at the cellular or subcellular levels are poorly defined. The mechanisms by which changes in cell shape are transduced to intracellular signals likely depend on receptor-mediated interactions with the cytoskeleton, but strain-associated transduction pathways may involve stretch-sensitive ion channels, G protein-dependent reactions, the action of locally produced autocrine or paracrine factors, or a combination of these factors. These observations suggest a general model of the response to pneumonectomy that may be used to formulate specific hypotheses as a basis for future investigations. This approach will provide insight into the mechanisms by which physical forces influence growth and metabolism in the lung and other tissues.
Modeling collisional processes in plasmas using discontinuous numerical methods (2016)

Thesis (Ph.D.)--University of Washington, 2016-06
Targeting cytokines in autoimmunity: new approaches, new promise (2003)

The increasing understanding of the pathophysiology of a number of human autoimmune diseases, and the realisation that cytokines play a major role, has provided the pharmaceutical industry with a wide array of new targets for therapeutic intervention. This has also resulted in a surge of interest for the development of ways of blocking cytokines and their actions in a specific and safe manner. This article reviews the current status of anticytokine therapy and the major efficacy that anti-TNF-a monoclonal antibodies (mAbs) and soluble TNF receptors have demonstrated in the clinic, which has led to their approval for the treatment of rheumatoid arthritis (RA), Crohn's disease (CD), juvenile arthritis and psoriatic arthritis. In addition, the development of novel approaches of cytokine blockade that are based on the characterisation of intracellular signalling pathways regulating cytokine expression (e.g., nuclear factor kappa B [NF-kB] and p38 mitogen activated protein kinase [MAPK]) and the use of small molecule inhibitors are discussed. Whether these approaches will keep up with their early promise and become a major and widespread treatment for several devastating autoimmune diseases will depend on specificity, safety, durability of the benefit, and pharmacoeconomic issues.
Synchronization Technique of Grid-Connected Power Converters Based on a Limit Cycle Oscillator (2017)

This paper shows a synchronization technique of power converters based on a limit cycle oscillator (LCO) and a frequency-locked loop (FLL), which makes the system frequency adaptive. The proposed technique LCO-FLL offers a high degree of immunity and robustness against swells, sags, harmonics, and phase-frequency shift. Furthermore, the LCO-FLL has the advantage of performing the synchrony with the grid from any initial condition set for LCO, ensuring an acceptable transient onto the synchronization process. Other advantages of the proposed scheme are that it does not require a phase-locked loop or trigonometric functions and it gives useful information to estimate the positive- and negative-sequence components in a three-phase system with a highly polluted grid scenario. In this paper, a local stability analysis of the LCO-FLL is presented. The behavior and performance of the proposed technique are compared with synchronization techniques by means of experimental results. Furthermore, a three-phase application of the synchronization technique is evaluated experimentally.
Determination of D-fructose in foodstuffs by an improved amperometric biosensor based on a solid binding matrix (1999)

An improved amperometric biosensor based on a solid binding matrix (SBM) composite transducer has been used for the determination of D-fructose in foodstuffs samples. The enzyme, D-fructose dehydrogenase (EC 1.1.99.11), was incorporated directly into a solid composite transducer containing both 2-hexadecanone as SBM and chemically modified graphite. Hexacyanoferrate(III) was used as a redox mediator and the current variation caused by the presence of D-fructose was measured amperometrically. The electrochemical properties and the characteristics of the composite fructose biosensors are described. The amperometric signals were fast, reproducible and linearly proportional to D-fructose concentrations in the range 50 × 10–6–10 × 10–3 mol l–1, with a correlation coefficient of 0.999. A set of measurements at +0.20 V versus SCE for 2 × 10–3 mol l–1D-fructose yielded a relative standard deviation for the steady-state current of 2.11%. The use of a chemically modified graphite by a mild oxidation step was shown to improve the biosensor selectivity against anionic interferents such as L-ascorbate. The biosensor proved to be stable for 6 months and the assay of D-fructose by this electrode was not influenced by the presence of sugars or other interferents commonly found in food samples. The biosensor was used for the determination of D-fructose in some food samples, and the results were consistent with those obtained with the commercially available D-fructose enzyme photometric kit.
BIOLOGICAL CONVERSION OF NARBONOLIDE TO PICROMYCIN (1973)

The macrolide antibiotic, picromycin, was found to be synthesized from narbonolide by the following two metabolic pathways: (A) Narbonolide (I) → narbomycin (II) → picromycin (III). (B) Narbonolide (I) → picronolide (IV) → picromycin (III). In path (A) glycosidation at C-5 with desosamine preceded hydroxylation at C-12, while in path (B) the reverse is true.
ChemInform Abstract: Palladium‐Catalyzed Cross‐Coupling Syntheses of Benzotriazolyl Enynes and a General Route to Enynyl Ketones and Alkynyl Ketones. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
A highly sensitive and specific chemiluminescent enzyme‐linked immunosorbent assay for diagnosis of active Trypanosoma cruzi infection (1997)

The chemiluminescent ELISAs provide a test that is highly sensitive (purified trypomastigote glycoconjugate and complex epimastigote antigens) and specific (purified trypomastigote glycoconjugate antigen) for Chagas' disease diagnosis. It can be used in blood bank screening and to monitor the treatment of patients undergoing chemotherapy.