gebhart/patrepeval huggingface dataset:
IgA Nephropathy: Characterization of IgG Antibodies Specific for Galactose-Deficient IgA1 (2007)

The circulating immune complexes in IgA nephropathy (IgAN) are composed of galactose (Gal)-deficient IgA1 bound to IgG or IgA1 antibodies specific for hinge-region O-linked glycans of Gal-deficient IgA1. To analyze properties of the anti-glycan antibodies, we determined the binding of serum IgG and IgG secreted by Epstein-Barr virus (EBV)- immortalized B cells from patients with biopsy-proven IgAN (n = 12) and healthy controls (n = 5) to a panel of antigens coated on ELISA plates. These antigens were: (1) enzymatically desialylated and degalactosylated IgA1 myeloma protein (dd-IgA1), (2) Fab fragment of Gal-deficient IgA1 containing part of the hinge region with O-glycans (Fab-IgA1), (3) synthetic hinge-region peptide linked to bovine albumin (HR-BSA), and (4) synthetic hingeregion glycopeptide with three GalNAc residues linked to BSA (HR-GalNAc-BSA). IgG-secreting EBV-immortalized cell lines were subcloned by limiting dilution. The concentration of total IgG and distribution of IgG subclasses were measured by ELISA. The levels of IgG in sera and supernatants directed against dd-IgA1 and Fab-IgA1 were significantly higher in IgAN patients than in controls (p < 0.01). IgG from IgAN patients exhibited strong reactivity with HR-GalNAc-BSA, but not with HR-BSA. The IgG-secreting cell lines produced antibodies specific to dd-IgA1; the antigen-specific IgG was most frequently of the IgG2 subclass. In summary, sera and supernatants from IgG-secreting cell lines from patients with IgAN were characterized by high levels of IgG antibodies with specificity to the Gal-deficient O-linked glycans of IgA1. The immortalized cell lines will provide a stable and convenient source of IgG for molecular studies of antibodies specific to the aberrant O-glycans in IgA1.
Ligand-dependent Corepressor LCoR Is an Attenuator of Progesterone-regulated Gene Expression (2009)

Ligand-dependent corepressor LCoR interacts with the progesterone receptor (PR) and estrogen receptor ERalpha in the presence of hormone. LCoR contains tandem N-terminal PXDLS motifs that recruit C-terminal-binding protein (CtBP) corepressors as well as a C-terminal helix-turn-helix (HTH) domain. Here, we analyzed the function of these domains in coregulation of PR- and ERalpha-regulated gene expression. LCoR and CtBP1 colocalize in nuclear bodies that also contain CtBP-interacting protein CtIP and polycomb group repressor complex marker BMI1. Coexpression of CtBP1 in MCF7 or T47D breast cancer cells augmented corepression by LCoR, whereas coexpression of CtIP did not, consistent with direct interaction of LCoR with CtBP1, but not CtIP. The N-terminal region containing the PXDLS motifs is necessary and sufficient for CTBP1 recruitment and essential for full corepression. However, LCoR function was also strongly dependent on the helix-turn-helix domain, as its deletion completely abolished corepression. LCoR, CtBP, and CtIP were recruited to endogenous PR- and ERalpha-stimulated genes in a hormone-dependent manner. Similarly, LCoR was recruited to estrogen-repressed genes, whereas hormone treatment reduced CtBP1 binding. Small interfering RNA-mediated knockdown of LCoR or CtBP1 augmented expression of progesterone- and estrogen-stimulated reporter genes as well as endogenous progesterone-stimulated target genes. In contrast, their ablation had gene-specific effects on ERalpha-regulated transcription that generally led to reduced gene expression. Taken together, these results show that multiple domains contribute to LCoR function. They also reveal a role for LCoR and CtBP1 as attenuators of progesterone-regulated transcription but suggest that LCoR and CtBP1 can act to enhance transcription of some genes.
Liver-specific expression of a Qa-encoded class I gene is associated with DNA hypomethylation. (1986)

DNA methylation of two murine major histocompatibility complex (H-2) class I genes was examined in hybridizations to MspI and HpaII chromosomal DNA restriction digests. Q10, which exhibits liver-specific expression, and H-2Kb, a transplantation antigen gene, were examined in liver, spleen, thymus, and cell-line DNAs. Unmethylated Q10 gene sequences were detected only in the liver, whereas the H-2Kb gene was unmethylated in all tissues examined.
Intimate association of Thy-1 and the T-cell antigen receptor with the CD45 tyrosine phosphatase. (1990)

Immunoprecipitation of Thy-1 from Triton X-100 detergent lysates of surface-iodinated and chemically cross-linked T cells precipitated at least five major and discrete bands. Four of these bands were identified as Thy-1, CD45 (a transmembrane tyrosine phosphatase), a major histocompatibility complex-encoded class I molecule, and beta 2-microglobulin. Similar analyses revealed that CD45 was coprecipitated from lysates of cross-linker-treated cells by antibodies to the T-cell antigen receptor (TCR). The same pattern of coprecipitated bands was observed when digitonin was used to lyse untreated cells. Immunoprecipitation of Thy-1 or the TCR from lysates of cross-linked T cells precipitated CD45 tyrosine phosphatase activity. Calculations based upon the amounts of coprecipitated enzymatic activity or TCR zeta chain indicate that a substantial fraction of Thy-1 and TCR complexes can be cross-linked to CD45. These data support a model in which the dependence of Thy-1 signaling on TCR coexpression is due to their common interaction with a tyrosine phosphatase and provide a possible structural basis for the influence of CD45 on TCR-mediated signaling.
Predicting Customer Churn Prediction In Telecom Sector Using Various Machine Learning Techniques (2018)

Customer churn analysis and prediction in telecom sector is an issue now a days because it's very important for telecommunication industries to analyze behaviors of various customer to predict which customers are about to leave the subscription from telecom company. So data mining techniques and algorithm plays an important role for companies in today's commercial conditions because gaining a new customer's cost is more than retaining the existing ones. In this paper we can focuses on various machine learning techniques for predicting customer churn through which we can build the classification models such as Logistic Regression, SVM, Random Forest and Gradient boosted tree and also compare the performance of these models.
Concentration difference energy systems (1978)

A new concept in electric power generation based on the absorption of steam into a thick solution which causes the generation of absorption heat (called the concentration difference energy system - CDE) is described. When heat is applied to water in an enclosure connected (above the liquid level) to a container of thick aqueous solution of salts, the difference in vapor pressure assures a constant flow of steam which can be used to turn a turbine to generate electricity. Many sources of low grade heat which can be harnessed by this CDE system are mentioned. (WHK)
The Evaluation Model on an Application of SROI for Sustainable Social Enterprises (2020)

The evaluation of social enterprises has been criticized for not being able to reflect the positive social aspects of the company through the financial evaluation. SROI (Social Return on Investment) is a social concept applied to the measurement of economic return on investment that aims to measure the social added value of social enterprises and reflect them in their performance. It is necessary to research for the provision of support and management for sustainable social enterprises, and to prepare a method for evaluating social enterprise by applying SROI. The Delphi 1st and 2nd surveys for the development of evaluation model for social enterprises using SROI were conducted by 50 social enterprise CEOs and experts. To produce the results of this study, the SPSS 20.0, AMOS 24 and Expert Choice 11 programs were used and the pairwise comparison analysis method was performed to analyze importance and priority. The results of the Delphi and AHP (Analytic Hierarchy Process) analysis showed that employment was the most important factor in social enterprises with the highest share of newly hired personnel. Employment-type social enterprises have the highest priority in terms of employment, income (income increase for vulnerable workers), and community contribution (affordable of social services), while the social service type was in order of employment, community contribution, and income (income increase for the vulnerable workers). On the other hand, the mixed type was employment (newly hired personnel), income (income increase of vulnerable workers), employment (social work participants' switch to similar work after contract expiration), and community contribution (affordable of social services). This study makes efforts to form social capital by raising the public's awareness of social value with efficient management through various evaluations of social enterprises and the emergence of various social enterprises. This study also emphasizes the need to better understand social enterprises as a multi-scholar and multi-dimensional organization that includes a multi-faced mechanism of social, economic, and environmental community development, away from understanding social enterprises as a specific business model.
Liposomes as a gene delivery system (1999)

Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle. This has encouraged the development of nonviral DNA-mediated gene transfer techniques such as liposomes. Many liposome-based DNA delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. Another recent technology using cationic lipids has been evaluated and has generated substantial interest in this approach to gene transfer.
Control Law Synthesis for Active Flutter Suppression Using Optimal Control Theory (1979)

This paper describes a study investigating the use of optimal control theory for the synthesis of an active flutter-suppression control law. For an example design application, a high-aspect-ratio cantilever wind-tunnel wing model is considered. The structural dynamics are represented by analytically computed natural frequencies and mode shapes. The three-dimensional unsteady aerodynamic forces for oscillatory motion are computed employing the doublet-lattice technique. With the aid of finite-order approximating functions for representing the aerodynamic forces in the time domain, the equations are written in the standard state vector form. Linear optimal control theory is then applied to find particular sets of gain values which minimize a quadratic cost function of the states and controls. These control laws are shown to increase the flutter dynamic pressure by at least 50% at Mach numbers 0.7 and 0.9. The closed-loop system's control surface activity in a gust environment is also examined.
A single‐step procedure for the isolation of individual mRNA species from crude lysates of <i>Physarum polycephalum</i> (1983)

An individual mRNA species was isolated directly from lysates of Physarum polycephalum by hybridization to immobilised complementary DNA. The solvent for lysis was a 6 M‐guanidinium salt solution, and the hybridization reaction was carried out at 42°C in 4 M guanidinium salt/33% (v/v) formamide buffer. The mRNA species isolated by this procedure was active in cell‐free protein synthesis and directed the synthesis of a polypeptide of M r 25 500 ± 750.
Drug-resistant human laryngeal carcinoma cells have increased levels of cathepsin B. (2001)

In our previous work we showed that the drug-resistance of cervical carcinoma, laryngeal carcinoma and glioblastoma cells may be accompanied by increased levels of tumor markers for invasion and metastasis (i.e. urokinase-type plasminogen activator, plasminogen activator inhibitor type 1, and/or cathepsin D). In the present study we examined the concentration of cathepsins B, L and H in three drug-resistant clones isolated from human laryngeal carcinoma (HEp2). The basal levels of cathepsins B, L and H were determined by enzyme linked immunoabsorbent assay (ELISA). Our results showed that all three clones had an increased level of cathepsin B (in two clones an almost 4-fold increase was determined). The level of cathepsin L was altered (increased) only in VK2 clone, while the levels of cathepsin H were similar in parental cells and drug-resistant clones. Thus, our results suggest that drug-resistance may be accompanied by an increased level of cathepsin B, i.e. tumor associated protease, involved in invasion and metastasis.
Silent Scaffolds (2012)

We established a new in vivo arrestin-3-JNK3 interaction assay based on bioluminescence resonance energy transfer (BRET) between JNK3-luciferase and Venus-arrestins. We tested the ability of WT arrestin-3 and its 3A mutant that readily binds β2-adrenergic receptors as well as two mutants impaired in receptor binding, Δ7 and KNC, to directly bind JNK3 and to promote JNK3 phosphorylation in cells. Both receptor binding-deficient mutants interact with JNK3 significantly better than WT and 3A arrestin-3. WT arrestin-3 and Δ7 mutant robustly promoted JNK3 activation, whereas 3A and KNC mutants did not. Thus, receptor binding, JNK3 interaction, and JNK3 activation are three distinct arrestin functions. We found that the KNC mutant, which tightly binds ASK1, MKK4, and JNK3 without facilitating JNK3 phosphorylation, has a dominant-negative effect, competitively decreasing JNK activation by WT arrestin-3. Thus, KNC is a silent scaffold, a novel type of molecular tool for the suppression of MAPK signaling in living cells.
Designing Call Auction Institutions: Is Double Dutch The Best? (1992)

Journal Article Designing Call Auction Institutions: Is Double Dutch The Best? Get access Kevin A. McCabe, Kevin A. McCabe University of Arizona Search for other works by this author on: Oxford Academic Google Scholar Stephen J. Rassenti, Stephen J. Rassenti University of Arizona Search for other works by this author on: Oxford Academic Google Scholar Vernon L. Smith Vernon L. Smith University of Arizona Search for other works by this author on: Oxford Academic Google Scholar The Economic Journal, Volume 102, Issue 410, 1 January 1992, Pages 9–23, https://doi.org/10.2307/2234848 Published: 01 January 1992
Isolation and Characterization of a Yeast<i>Cryptococcus</i>sp. S-2 That Produces Raw Starch-digesting<i>α</i>-Amylase, Xylanase, and Polygalacturonase (1994)

Journal Article Isolation and Characterization of a Yeast Cryptococcus sp. S-2 That Produces Raw Starch-digesting α-Amylase, Xylanase, and Polygalacturonase Get access Haruyuki Iefuji, Haruyuki Iefuji National Research Institute of Brewing, 2–6–30, Takinogawa, Kita-ku, Tokyo 114, Japan Search for other works by this author on: Oxford Academic Google Scholar Yuzuru Iimura, Yuzuru Iimura National Research Institute of Brewing, 2–6–30, Takinogawa, Kita-ku, Tokyo 114, Japan Search for other works by this author on: Oxford Academic Google Scholar Takaji Obata Takaji Obata National Research Institute of Brewing, 2–6–30, Takinogawa, Kita-ku, Tokyo 114, Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 58, Issue 12, 1 January 1994, Pages 2261–2262, https://doi.org/10.1271/bbb.58.2261 Published: 01 January 1994 Article history Published: 01 January 1994 Received: 02 May 1994
3-D surface solution using structured light and constraint propagation (1989)

A method for 3-D surface measurement using a projected grid of light is presented. The grid line identification problem is solved using very general constraints. At the same time, 3-D surface patch solutions are developed. From the general constraints of set of geometric and topological rules are derived that are effectively applied in the computation of grid labels and hence 3-D surface solutions. A set of five algorithms has been applied on five real scenes consisting of multiple objects of arbitrary shapes. The results show that globally consistent surface solutions can be obtained rapidly with good accuracy using a single image. A small degree of ambiguity remains, but can be further reduced or removed using increased knowledge.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Forced reconnection and mode locking in rotating cylindrical plasmas (1997)

The problem of mode locking due to forced magnetic reconnection in rotating cylindrical plasmas is revisited. Forced reconnection is characterized by very large values of the parameter Δ′, which makes the constant-ψ approximation generally inapplicable in the linear and early nonlinear regimes. The nonlinear dynamics of rotating non-constant-ψ islands is distinguished by the persistence of current sheets spanning Y points. Mode locking due to a suddenly imposed error field is discussed. Temporal dynamics and locking thresholds that differ significantly from the predictions of the constant-ψ theory [R. Fitzpatrick and T. C. Hender, Phys. Fluids B 3, 644 (1991)] are obtained. The predictions of the present theory are compared with experimental tokamak observations.
Reinvestigation of Aminomethyltrifluoroborates and Their Application in Suzuki−Miyaura Cross-Coupling Reactions (2011)

A reinvestigation into the chemical composition of potassium aminomethyltrifluoroborates is reported. These trifluoroborato salts have been reassigned as zwitterionic ammoniomethyltrifluoroborates. Minor adjustments to the previously disclosed reaction conditions are reported that permit a similar level of activity as nucleophiles in Suzuki-Miyaura cross-coupling reactions.
Differential activation of stress-activated protein kinase kinases SKK4/MKK7 and SKK1/MKK4 by the mixed-lineage kinase-2 and mitogen-activated protein kinase kinase (MKK) kinase-1 (1998)

Overexpression of the protein kinases mixed-lineage kinase-2 (MLK2) or mitogen-activated protein kinase (MAPK) kinase kinase-1 (MEKK1) is known to trigger the activation of stress-activated protein kinase (SAPK1)/c-Jun N-terminal kinase (JNK). Here we demonstrate that MLK2 activates SAPK kinase-1 (SKK1)/MAPK kinase 4 (MKK4) and SKK4/MKK7, the two known direct activators of SAPK1/JNK (both in transfection studies and in vitro). In contrast, MEKK1 activates SKK1/MKK4 more efficiently than MLK2, but barely activates SKK4/MKK7. Since SKK4/MKK7 (but not SKK1/MKK4) is activated by interleukin-1 and tumour necrosis factor in several cells and tissues, we suggest that MEKK1 does not mediate the activation of SKK4/MKK7 and SAPK1/JNK induced by these pro-inflammatory cytokines. MLK2 and MEKK1 also activated SKK2/MKK3 and SKK3/MKK6, the direct upstream activators of SAPK2a/p38.
The influence of head parameters on take-off velocity (1993)

The dependence of take-off velocity (TOV) on selected head parameters was measured with an air bearing spindle equipped with a strain gauge. Considering the range that was studied for each parameter, TOV was found to be strongly dependent on crown, skew angle, ski jump and suspension preload, in decreasing order. Camber height and rail width were found to have a lesser influence. Edge blend did not affect TOV; however, increasing edge blend improved the head-disk performance in contact start-stop (CSS) tests.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Blood coagulation changes in mice bearing Lewis lung carcinoma, a metastasizing tumor. (1977)

In view of the possible role of platelets and coagulation mechanisms in the growth and dissemination of solid tumors, a number of hematological parameters were followed during development of an experimental syngeneic tumor in mice, Lewis lung carcinoma. This tumor, when transplanted i.m. in C57BL/6 mice, grows locally and spontaneously metastasizes to the lungs. The transplanted animals survive for about 4 weeks. Metastases are visible from the third week. A slight but constant increase in plasma fibrinogen level and marked thrombocytopenia were first observed during the second week after tumor implantation. No other significant changes in coagulation and fibrinolysis parameters were detected. Moreover, the animals developed marked hemolytic anemia, possibly microangiopathic in origin. 125I-labelled fibrinogen survival was decreased by about 20% during the second week after tumor implantation and was not further reduced later. Fibrinogen turnover was progressively accelerated, being more than doubled by the end of the third week. Labeled fibrinogen accumulated in the primary tumor and in the lungs (its rate of disappearance from the tumor was much slower than that from lungs or blood). 51Cr-labeled platelet survival did not change throughout the observation period, whereas platelet turnover was markedly reduced from the end of the second week, suggesting defective platelet production. 51Cr-labeled RBC survival was drastically reduced to about 30% of the controls starting from the second week. The occurence of low-grade, localized intravascular coagulation could be suggested on the basis of these data. Moreover, when Lewis lung carcinoma cells were abruptly injected i.v. through the tall vein, more impressive signs of intravascular coagulation could be seen. Indeed, there was a rapid decrease in the number of platelets, a reduction in fibrinogen, and an increase in fibrin-fibrinogen degradation products. The effects of i.v. injection of Lewis lung carcinoma cells indicate a relevant interference of cancer cells with the hematostatic system. In contrast, the tenuous evidence fo coagulation disorders in animals receiving injections of tumor cells i.m. seems to indicate a limited effect on hemostasis of the same cells during i.m. tumor growth.
Credit Risk Evaluation Based on Social Media (2015)

Social media has been playing an increasingly important role for individuals to share their own opinions on many financial issues including credit risk in the investment decision. This paper analyzes whether these opinions transmitted through social media can accurately predict enterprises’ future credit risk. We consider financial statements oriented evaluation results based on logit method as the benchmark. And we then conduct textual analysis to retrieve both posts and their corresponding commentaries published on two most popular social media platforms of financial investors in China. The professional advice from financial analysts is also investigated in this paper. We surprisingly find that the opinions extracted from both posts and commentaries surpass opinions of analysts in terms of credit risk prediction.
Multispectral ultrasound imaging and analysis of speckle generating medium (2002)

The use of FM pulse imaging scheme to obtain multispectral data from a speckle generating medium is discussed. Its potential for speckle reduction was evaluated by computing correlation coefficients. Measured coefficients were also compared with model predictions. The results are consistent with other published reports and show that significant speckle decorrelation can be achieved with this technique, if one averages frames acquired with center frequencies at the extreme ends of the transducer operating bandwidth, and a small enough /spl Delta/f. At these extremes, the transducer response is low. This response limitation can, however, be overcome by increasing the linear frequency sweep time duration in the FM pulse imaging scheme. In a frequency dependent attenuating medium, proper choices of f/sub o/ and /spl Delta/f spanning the entire operating bandwidth of transducer may be necessary. The flexibility inherent in the FM pulse scheme can be exploited here.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>