gebhart/patrepeval huggingface dataset:
Reversible sodium pump defect and swelling in the diabetic rat erythrocyte: effects on filterability and implications for microangiopathy. (1989)

We have found a defect in the ouabain-sensitive Na+, K+-ATPase (Na+ pump, EC 3.6.1.37) of erythrocytes from streptozocin diabetic rats. This defect was accompanied by an increase in cell volume and osmotic fragility and a decrease in the cytosolic K+/Na+ ratio. There was also a doubling in the time needed for diabetic erythrocytes to pass through 4.7-micron channels in a polycarbonate filter. Our data are consistent with a primary defect in the erythrocyte Na+ pump and secondary changes in cell volume, osmotic fragility, K+/Na+ ratio, and cell filterability. All were reversed or prevented in vivo by insulin or the aldose reductase inhibitor Sorbinil. Protein kinase C agonists (phorbol ester and diacylglycerol) and agonist precursor (myoinositol) reversed the Na+ pump lesion, suggesting that protein kinase C-dependent phosphorylation of the 100-kDa subunit regulates Na+ pump activity and that insulin can influence erythrocyte protein kinase C activity. Ouabain inhibition of the erythrocyte Na+ pump also produced increases in cell size and reductions in rates of filtration. Theoretical treatment of the volume changes also predicts reduction in filterability as a consequence of cell swelling. We suggest that enlarged erythrocytes could play a role in the evolution of the microvascular changes of diabetes mellitus.
Chemical and Behavioral Ecology of Palm Weevils (Curculionidae: Rhynchophorinae) (1996)

R. M. Giblin-Davis, A. C. Oehlschlager, A. Perez, G. Gries, R. Gries, T. J. Weissling, C. M. Chinchilla, , R. H. Hallett, H. D. Pierce, Jr., L. M. Gonzalez, Chemical and Behavioral Ecology of Palm Weevils (Curculionidae: Rhynchophorinae), The Florida Entomologist, Vol. 79, No. 2 (Jun., 1996), pp. 153-167
Secondary Vibrio cholerae-specific cellular antibody responses following wild-type homologous challenge in people vaccinated with CVD 103-HgR live oral cholera vaccine: changes with time and lack of correlation with protection (1993)

Peripheral blood immunoglobulin A antibody-secreting-cell (ASC) responses are thought to reflect the mucosal immune response to locally presented antigens. We evaluated the ASC response to cholera toxin (CT) and Inaba lipopolysaccharide (LPS) in 26 North American volunteers following immunization with a single oral dose of live attenuated Vibrio cholerae O1 vaccine strain CVD 103-HgR and again upon homologous wild-type challenge with V. cholerae classical Inaba 569B. Challenge occurred at either 7, 30, or 180 days after vaccination. The CT and LPS ASC responses of volunteers following vaccination (83 and 55%, respectively) were similar in magnitude and frequency to those of unvaccinated controls following wild-type challenge (80 and 60%, respectively [0.1 < or = P < or = 0.9]). The responses were primarily immunoglobulin A. Vaccinated volunteers challenged within 30 days of vaccination had reduced or nondetectable CT and LPS ASC responses. Challenge at 6 months resulted in a heightened ASC response to LPS, confirming the existence of mucosal memory. ASC responses to CT upon challenge at 6 months were detectable but not different from that seen following primary immunization, suggesting that secondary ASC responses to different antigens from a single vaccine operate independently. In spite of these variable ASC responses, the vaccine efficacy was 100% following challenge for all vaccinees. V. cholerae-specific ASC responses following antigenic reexposure gave information on the presence of mucosal B memory cells but did not correlate with protective immunity. As such, these ASC assays will have limited usefulness for evaluating vaccine responders in vaccine field trials in cholera-endemic areas where prior V. cholerae O1 exposure is unknown.
Consideration of the evolution of the <i>Saccharomyces cerevisiae MEL</i> gene family on the basis of the nucleotide sequences of the genes and their flanking regions (1994)

Analysis of the DNA sequences of new members of the Saccharomyces cerevisiae MEL1-MEL10 gene family showed high homology between the members. The MEL gene family, alpha-galactosidase-coding sequences, have diverged into two groups; one consisting of MEL1 and MEL2 and the other of MEL3-MEL10. In two S. cerevisiae strains containing five or seven MEL genes each, all the genes are nearly identical, suggesting very rapid distribution of the gene to separate chromosomes. The sequence homology and the abrupt change to sequence heterogeneity at the centromere-proximal 3' end of the MEL genes suggest that the distribution of the genes to new chromosomal locations has occurred partly by reciprocal recombination at solo delta sequences. We identified a new open reading frame sufficient to code for a 554 amino acid long protein of unknown function. The new open reading frame (Accession number Z37509) is located in the 3' non-coding region of MEL3-MEL10 genes in opposite orientation to the MEL genes (Accession numbers Z37508, Z37510, Z37511). Northern analysis of total RNA showed no hybridization to a homologous probe, suggesting that the gene is not expressed efficiently if at all.
<i>Campylobacter jejuni</i>in dairy cows and raw milk (1987)

Twelve herds of dairy cows were examined by rectal swabbing for the presence of Campylobacter jejuni. Ten herds were positive with the incidence of colonized animals ranging from 10 to 72% of those tested. With the exception of the two negative herds where mains water only was consumed, all animals drank from rivers or streams when grazing. There was no relationship between total and coliform counts and the presence of C. jejuni in raw milk. However, milk from one farm that consistently gave positive results had significantly higher Escherichia coli counts than other samples.
A polyethnic London carnival as a contested cultural performance* (1982)

Click to increase image sizeClick to decrease image size Notes The material on which this article is based was gathered with the help of a research grant from the SSRC of Great Britain, for which I am grateful. I owe a special debt of gratitude to the many leaders and artists of the Notting Hill Carnival for giving me so much of their time in discussion. I would like also to record my thanks to Dr Helen Hornsey for valuable assistance in gathering some of the records.
A novel power combining configuration without using an isolator (2002)

A power combining circuit configuration not requiring an isolator is presented. As the configuration enables all orders of intermodulation to be reduced, it promises to contribute significantly to the miniaturization of millimeter-wave communication equipment.
Flow injection analysis of three N-substituted phenothiazine drugs with amperometric detection at a carbon fibre array electrode (1985)

The carbon fibre array electrode is demonstrated as a useful detector for flow injection analysis in the amperometric mode of detection.A liquid chromatographic internal sampling valve was used to inject the sample into the supporting electrolyte stream and sampling rates as high as 200 samples per hour were feasible using a flow-rate of 1 ml min–1. The oxidations of three pharmaceutically important phenothiazine drugs, namely perphenazine, triflupromazine. HCI and fluphenazine.2HCI, were investigated and an assay procedure was developed for these compounds and their formulations. Detection limits for the analytes were a few tenths of ananogram.
DNA modification: intrinsic selectivity of nickel(II) complexes (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDNA modification: intrinsic selectivity of nickel(II) complexesXiaoying Chen, Steven E. Rokita, and Cynthia J. BurrowsCite this: J. Am. Chem. Soc. 1991, 113, 15, 5884–5886Publication Date (Print):July 1, 1991Publication History Published online1 May 2002Published inissue 1 July 1991https://pubs.acs.org/doi/10.1021/ja00015a065https://doi.org/10.1021/ja00015a065research-articleACS PublicationsRequest reuse permissionsArticle Views233Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
When Cooperation Fails: The International Law and Politics of Genetically Modified Foods (2009)

The dispute over genetically modified organisms has brought the US & the EU into conflict. This book examines the dynamic interactions of domestic law & politics, transnational networks, international regimes, & global markets, through a theoretically grounded & empirically comprehensive analysis of the governance of GM foods & crops.
Techniques in Molecular Systematics and Evolution (2002)

I Analytical Methods.- to Part I.- 1 Phylogenetic Analysis: The Basic Method.- 2 Parsimony Analysis.- 3 Optimization Alignment: Down, Up, Error, and Improvements.- 4 Techniques for Analyzing Large Data Sets.- 5 Measures of Support.- 6 Partitioning of Multiple Data Sets in Phylogenetic Analysis.- 7 Complex Model Organism Genome Databases.- 8 Comparative Phylogenomics: A Strategy for High-throughput Large-scale Sub-genomic Sequencing Projects for Phylogenetic Analysis.- 9 Comparative Methods.- 10 Analyzing Data at the Population Level.- II Laboratory Methods.- to Part II.- 11 Obtaining, Storing and Archiving Specimens and Tissue Samples for Use in Molecular Studies.- 12 DNA Isolation Procedures.- 13 Late Pleistocene DNA Extraction and Analysis.- 14 PCR Methods and Approaches.- 15 High-Throughput DNA Sequencing for Systematics.- 16 A Practical Guide for Microsatellite Analysis.- 17 Determining the Spatial and Temporal Patterns of Developmental Gene Expression in Vertebrates and Invertebrates Using in situ Hybridization Techniques.- Guide to Solutions.- Guide to protocols.- Troubleshooting Guide.
An approach for mining web service composition patterns from execution logs (2010)

A service-oriented application is composed of multiple web services to fulfill complex functionality that cannot be provided by individual web service. The combination of services is not random. In many cases, a set of services are repetitively used together in various applications. We treat such a set of services as a service composition pattern. The quality of the patterns is desirable due to the extensive uses and testing in the large number of applications. Therefore, the service composition patterns record the best practices in designing and developing reliable service-oriented applications. The execution log tracks the execution of services in a service-oriented application. To document the service composition patterns, we propose an approach that automatically identifies service composition patterns from various applications using execution logs. We locate a set of associated services using Apriori algorithm and recover the control flows among the services by analyzing the order of service invocation events in the execution log. A case study shows that our approach can effectively detect service composition patterns.
Outcomes Following Acute Exacerbation of Severe Chronic Obstructive Lung Disease. The SUPPORT Investigators (Study to Understand Prognoses and Preferences for Outcomes and Risks of Treatments) (1996)

In order to describe the outcomes of patients hospitalized with an acute exacerbation of severe chronic obstructive pulmonary disease (COPD) and determine the relationship between patient characteristics and length of survival, we studied a prospective cohort of 1,016 adult patients from five hospitals who were admitted with an exacerbation of COPD and a PaCO2 of 50 mm Hg or more. Patient characteristics and acute physiology were determined. Outcomes were evaluated over a 6 mo period. Although only 11% of the patients died during the index hospital stay, the 60-d, 180-d, 1-yr, and 2-yr mortality was high (20%, 33%, 43%, and 49%, respectively). The median cost of the index hospital stay was $7,100 ($4,100 to $16,000; interquartile range). The median length of the index hospital stay was 9 d (5 to 15 d). After discharge, 446 patients were readmitted 754 times in the next 6 mo. At 6 mo, only 26% of the cohort were both alive and able to report a good, very good, or excellent quality of life. Survival time was independently related to severity of illness, body mass index (BMI), age, prior functional status, PaO2/FI(O2), congestive heart failure, serum albumin, and the presence of cor pulmonale. Patients and caregivers should be aware of the likelihood of poor outcomes following hospitalization for exacerbation of COPD associated with hypercarbia.
Electrical conductivity relaxation in thin-film yttria-stabilized zirconia (2001)

We report on complex admittance measurements on ZrO2:Y2O3 (YSZ) thin films in the parallel plate geometry. Highly textured YSZ thin films, grown by rf sputtering, allow measuring complex admittance free of the effect of charge blocking at grain boundaries. We have examined low-temperature (close to room temperature) regime dominated by association of oxygen vacancies. Complex admittance analyzed in terms of the modulus formalism supplies information on correlation effects in ion motion and allows obtaining an association energy for the oxygen vacancies of 0.45 eV, in agreement with previous theoretical calculations.
High-Performance Nano-Photoinitiators with Improved Safety for 3D Printing (2017)

In this work, we report the first hybrid nanosized photoinitiators with low cytotoxicity and migration by coupling of polyhedral oligomeric silsesquioxanes (POSS) to benzophenone derivatives. This new series of photoinitiators were fully characterized and showed many favorable properties such as uniform sizes, extremely low tendency to migrate, less effect on resin viscosity, enhanced thermal stability and mechanical strength, increased photoactivity, and significantly lower cell toxicity compared to their corresponding benzophenone molecules. The utility of these hybrid nanosized photoinitiators in 3D printing was demonstrated in printing of various 3D structures with high resolution and accuracy.
Comparación de dos procedimientos para la resincronización del estro de vacas inseminadas, previamente tratadas por anestro (2015)

In order to compare two methods for resynchronization (ESR) an \ninvestigation was carried out. For six months (November-April \n2008) 1088 crossbred cows were subjected to treatment estrus \ninduction (IE) and inseminated (AI) and divided into three groups, \ngroup A (n = 500) received an injection of 100 oily progesterone \nmg intramuscularly on day 13 after the service and 1 mg estradiol \nbenzoate 20 ± 1 days after AI. The B (n = 396), an oil solution of \nestradiol benzoate in 1mg 20 ± 1 day after AI and C, (n = 192) \nwitness. There were no significant differences between the first \nand second service although the conception rate of the latter was \nsuperior services p> 0.05 in comparison to the control group. CSR \ntreatment based on progesterone and estradiol benzoate was the \nmost efficient method
연구논문 : 고등학교 지구과학 교과과정의 개선을 위한 판구조론 개념의 비교분석 (1988)

The plate tectonics is the theory which can systematically interpret various kinds of changes of crusts on the earth surfaces. If the concepts of this theory is taken a triangular position and systematically played up at the curricula of the earth science in the high school, this theory may be able to become the most adequate subject for students to interest in the earth science. The aim of this study is the making out the expanded model of the unit of plate tectonics for the purpose of the more effective teaching-learning of the plate tectonics and the improvement of curricula on the plate tectonics in the high school.
Efficacy of oral afoxolaner for the treatment of canine generalised demodicosis (2016)

The efficacy of oral treatment with a chewable tablet containing afoxolaner 2.27% w/w (NexGard(®), Merial) administered orally was assessed in eight dogs diagnosed with generalised demodicosis and compared with efficacy in eight dogs under treatment with a topical combination of imidacloprid/moxidectin (Advocate(®), Bayer). Afoxolaner was administered at the recommended dose (at least 2.5 mg/kg) on Days 0, 14, 28 and 56. The topical combination of imidacloprid/moxidectin was given at the same intervals at the recommended concentration. Clinical examinations and deep skin scrapings were performed every month in order to evaluate the effect on mite numbers and the resolution of clinical signs. The percentage reductions of mite counts were 99.2%, 99.9% and 100% on Days 28, 56 and 84, respectively, in the afoxolaner-treated group, compared to 89.8%, 85.2% and 86.6% on Days 28, 56 and 84 in the imidacloprid/moxidectin-treated group. Skin condition of the dogs also improved significantly from Day 28 to Day 84 in the afoxolaner-treated group. Mite reductions were significantly higher on Days 28, 56 and 84 in the afoxolaner-treated group compared to the imidacloprid/moxidectin-treated group. The results of this study demonstrated that afoxolaner, given orally, was effective in treating dogs with generalised demodicosis within a two-month period.
Preparation and characterization of size-controlled silver nanoparticles decorated multi-walled carbon nanotubes and their electrocatalytic reduction properties for hydrogen peroxide (2013)

The well dispersed and size-controlled Ag nanoparticles decorated multi-walled carbon nanotubes (MWCNT) were synthesized by a simple chemical plating method. The oxidized MWCNT surfaces could get a more controlled and specific nucleation of Ag nanoparticles. The as-prepared nanocomposites were characterized by transmission electron microscopy, X-ray diffraction and cyclic voltammetry. Randles-Sevcik plot suggested that the reaction of the nanocomposites in alkaline solution was a diffusion-controlled process. The electrocatalytic reduction property toward H2O2 was also studied.
Assessment of the Performance of Acoustic and Mass Balance Methods for Leak Detection in Pipelines for Transporting Liquids (2010)

On-line leak detection is a main concern for the safe operation of pipelines. Acoustic and mass balance are the most important and extensively applied technologies in field problems. The objective of this work is to compare these leak detection methods with respect to a given reference situation, i.e., the same pipeline and monitoring signals acquired at the inlet and outlet ends. Experimental tests were conducted in a 749 m long laboratory pipeline transporting water as the working fluid. The instrumentation included pressure transducers and electromagnetic flowmeters. Leaks were simulated by opening solenoid valves placed at known positions and previously calibrated to produce known average leak flow rates. Results have clearly shown the limitations and advantages of each method. It is also quite clear that acoustics and mass balance technologies are, in fact, complementary. In general, an acoustic leak detection system sends out an alarm more rapidly and locates the leak more precisely, provided that the rupture of the pipeline occurs abruptly enough. On the other hand, a mass balance leak detection method is capable of quantifying the leak flow rate very accurately and of detecting progressive leaks.
False discovery rate, sensitivity and sample size for microarray studies (2005)

We use a mixture model, involving differentially expressed (DE) and non-DE genes, that captures the most common problem of finding DE genes. Factors determining FDR are (1) the proportion of truly differentially expressed genes, (2) the distribution of the true differences, (3) measurement variability and (4) sample size. Many current small microarray studies are plagued with large FDR, but controlling FDR alone can lead to unacceptably large FNR. In evaluating a design of a microarray study, sensitivity or FNR curves should be computed routinely together with FDR curves. Under certain assumptions, the FDR and FNR curves coincide, thus simplifying the choice of sample size for controlling the FDR and FNR jointly.
Characterization of human mineralocorticosteroid receptor expressed in the baculovirus system. (1991)

To investigate the structure and function of the mineralocorticosteroid receptor (MR), one has to circumvent the major difficulty related to its very low abundance. For this purpose, the full-length human MR (hMR) has been produced using the efficient baculovirus system. The recombinant protein is overexpressed in Sf9 insect cells at a concentration of approximately 2 pmol/mg of protein, which is 50-100 times more than the concentration in aldosterone target tissues. It binds aldosterone with high affinity (Kd approximately 1 nM) and clearly displays a mineralocorticosteroid specificity as evidenced by competition studies with steroid ligands and by the monoclonal anti-idiotypic antibody H10E interacting with the steroid-binding domain of MR. After [35S]methionine labeling, a single polypeptide band at approximately 120 kDa is detected and further identified as hMR by immunoblotting with A4, an anti-peptide antibody. Sedimentation analyses show that the native form of MR is recognized by A4 and B174, an antibody to the 90-kDa heat shock protein, since they both induce a shift of the receptor from 9S to 11S sedimentation coefficient. These results clearly demonstrate that MR is a heterooligomer containing the insect equivalent of the 90-kDa heat shock protein. This 9S receptor complex is converted to a 4S form during high-salt gradient ultracentrifugation, suggesting that MR can undergo a complete in vitro transformation. Immunofluorescence studies indicate that hMR, which is almost exclusively a cytoplasmic protein in Sf9 cells, is translocated to the nucleus after aldosterone exposure. Therefore, the recombinant hMR seems to behave as the native receptor. Given the possibility of large-scale protein production, the baculovirus system should prove useful in studies of the molecular basis of MR function as a transcription factor.
METHODS OF MEASURING SWIMMING SPEED OF SPERMATOZOA (1975)

Three basic approaches for determining the mean swimming speed of a suspension of microorganisms were compared, using bull and ram spermatozoa. Number fluctuation counting was performed automatically on a Quantimet 720 image analysing computer, the mean speed being obtained using 'probability after' statistics. The other two approaches were photomicrographic: number flux counting was performed on single photomicrographs; on the same photomicrographs, the mean speed was estimated from measurement of 'whole' and 'half' track lengths. These results were compared with each other and with the Quantimet results. The 'probability after' method was also compared, on additional samples, with cine-photomicrographic tracking. The mean speeds predicted by the 'probability after' method compared favourably with the other methods (range 68 mum/sec to 162 mum/sec). The results also suggested that, on single photomicrographs, measurement of 'half' track lengths or number flux counting were generally preferable to measurement of whole track lengths.