gebhart/patrepeval huggingface dataset:
Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA-E, F, and G in Maternal-Placental Immune Recognition (2003)

In this study we focused on the structure and expression of the HLA-E, F, and G class I complexes in placental tissue. Structural analysis included an examination of the peptides bound to soluble and membrane forms of the HLA-G complex isolated directly from placenta. An important distinction was observed from HLA-G bound peptides previously isolated from transfectant cells. Thus, the number of distinct moieties bound to placental-derived proteins was substantially lower than that bound to transfectant-derived HLA-G. Indeed, a single peptide species derived from a cytokine-related protein alone accounted for 15% of the molar ratio of HLA-G bound peptide. To further examine HLA-E and its potential to bind peptide, notably that derived from HLA-G, we combined new Abs to examine expression in placental tissues for all the known forms of the nonclassical class I molecules. Whereas membrane HLA-G was found in extravillous trophoblasts, soluble HLA-G was found in all placental trophoblasts, including villous cytotrophoblasts and syncitiotrophoblasts. Further, HLA-E was found in all cells that expressed either form of HLA-G, consistent with HLA-E being complexed with the HLA-G signal sequence-derived nonamer in these cells. Finally, using new reagents specific for HLA-F, a restricted pattern of expression was observed, primarily on extravillous trophoblasts that had invaded the maternal decidua. Comparative staining indicated that HLA-F was on the surface of these cells, defining them as the first to demonstrate surface expression of this Ag and the first cell type identified to express all three nonclassical HLA class I Ags simultaneously.
Silicon roughness induced by plasma etching (1994)

A parametric study of single-crystal silicon roughness induced by an SF6 plasma has been carried out by means of atomic force microscopy. An helicon source (also called resonant inductive plasma etcher) has been used to study the relation between plasma parameters and subsequent surface damage. The surface damage has been examined in terms of height roughness analysis and in terms of spatial (lateral) extent of the surface roughness. The central result is that roughness scales with the ratio of the ion flux over the reactive neutral flux (J+/JF), showing the combined role of both ionic and neutral species. At low ion flux, the neutrals smooth the surface, while at higher ion flux, they propagate the ion-induced defects, allowing the roughness to be enhanced. Influences of other parameters such as exposure duration, ion energy, or substrate temperature have also been quantified. It is shown that the roughness growth is well described by an empirical law: rms∝(1/√E)(J+/JF)ηtβ, with η≊0.45 and β≊1 (rms is the root mean square of the roughness). In other respects, we analyze the data with a Fourier transform analysis. The main advantage is to minimize noise and to separate the magnitude of the roughness, the lateral correlation length on which the roughness is growing, and the behavior of short and long range roughness. The results are identical to the rms analysis, especially, the above scaling law. The time evolution of the lateral correlation length follows a scaling law (which is not accessible by means of the rms) leading to a fractal dimension of 2.67. Also is observed a variation of the short range roughness as a function of the substrate bias voltage. Consequence for further scaling down of integrated circuits is called to mind.
The Good Life (2014)

Are we born selfish or primed to help others? Does stress make people more antisocial? Can we ever be genuinely altruistic? This book explores some of the dilemmas at the heart of being human. Integrating cutting edge studies with in-depth clinical experience, Graham Music synthesizes a wealth of fascinating research into an explanation of altruism, cooperation and generosity and shows how we are primed to turn off the ‘better angels of our nature’ in the face of stress, anxiety and fear. Using fascinating psychological research but rooted in a clinicians understanding of the impact of stress on our moral and pro-social capacities, The Good Life covers topics as diverse as: The role of parenting and family life in shaping how antisocial or pro-social we become How stress, abuse and insecure attachment profoundly undermine empathic and altruistic capacities The relative influence of our genes or environments on becoming big-hearted or coldly psychopathic How our immediate contexts and recent social changes might tilt us towards either selfish or cooperative behaviour This book makes a unique contribution to a subject that is increasingly on people’s minds. It does not shirk complexity, nor suggest easy explanations, but offers a hard look at the evidence in the hope that we can gain some understanding of how a ‘Good Life’ might develop. Often personally challenging, intellectually exhilarating and written with an easily accessible style, The Good Life makes sense of how our moral selves take shape, and shines a light on the roots of goodness and nastiness.
Cocaine use disorder prevalence: From current DSM-IV to proposed DSM-5 diagnostic criteria with both a two and three severity level classification system. (2013)

This article presents a secondary analysis from a study investigating the compatibility of the current DSM-IV and previously proposed DSM-5 cocaine use disorder (CUD) criteria (S. L. Proctor, A. M. Kopak, & N. G. Hoffmann, 2012, Compatibility of current DSM-IV and proposed DSM-5 diagnostic criteria for cocaine use disorders. Addictive Behaviors, 37, 722-728). The current analyses examined the compatibility of the current DSM-IV and two sets of proposed DSM-5 diagnostic criteria for CUDs among adult male inmates (N = 6,871) recently admitted to the Minnesota Department of Corrections state prison system from 2000-2003. Initially proposed DSM-5 criteria (DSM-5.0) featured only two diagnostic designations (i.e., moderate and severe). A subsequent revision (DSM-5.1) included the addition of a mild designation and required a greater number of positive findings for the severe designation. A computer-prompted structured diagnostic interview was administered to all inmates as part of routine clinical assessments. The past 12-month prevalence of DSM-IV CUDs was 12.70% (Abuse, 3.78%, Dependence, 8.92%), while 10.98% met past 12-month DSM-5.1 criteria for a CUD (Mild [MiCUD], 1.72%; Moderate [MCUD], 1.12%; and Severe [SCUD], 8.14%). The vast majority of those with no diagnosis (99.6%) continued to have no diagnosis, and most of those with a dependence diagnosis (91.2%) met SCUD criteria of the proposed DSM-5.1. Most of the variation in DSM-5.1 diagnostic classifications was accounted for by those with a current abuse diagnosis. DSM-5.0 MCUD cases were most affected when DSM-5.1 criteria were applied. The proposed diagnostic changes might translate to reduced access to treatment for those individuals evincing symptoms consistent with DSM-IV cocaine abuse.
Antitumor and antileukemic effects of some steroids and other biologically interesting compounds containing an alkylating agent (1972)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntitumor and antileukemic effects of some steroids and other biologically interesting compounds containing an alkylating agentF. I. Carroll, Abraham Philip, J. T. Blackwell, D. Jane Taylor, and Monroe E. WallCite this: J. Med. Chem. 1972, 15, 11, 1158–1161Publication Date (Print):November 1, 1972Publication History Published online1 May 2002Published inissue 1 November 1972https://pubs.acs.org/doi/10.1021/jm00281a016https://doi.org/10.1021/jm00281a016research-articleACS PublicationsRequest reuse permissionsArticle Views100Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Controlling fluid animation with geometric potential (2004)

Abstract We propose a new fluid control technique that uses a geometrically induced potential field. Instead of optimizing the control forces exerted at each frame, as was done in previous work, a potential is added as an extra dimension to the simulation space which coerces the fluid inside this space to form the target shape. This type of shape control requires practically no additional computing by the Navier‐Stokes solver at run‐time, and adds little overhead to implementation. The confinement potentials are induced from geometric information given by animators, and so the control forces that take fluids to a lower potential can be decided in a preprocessing step. We show that a slightly generalized Navier‐Stokes equation for fluids in potential fields can be simulated without changing the solver itself. A harmonic potential function can be quickly found with the Poisson solver which is already implemented as a part of the Navier‐Stokes solver. 2 and 3 dimensional flows designed by common methods such as hand drawing, traditional shape modeling and key‐framing, can be animated efficiently with our control technique. Copyright © 2004 John Wiley & Sons, Ltd.
METROPOLE-3D: An Efficient and Rigorous 3D Photolithography Simulator (1999)

this paper isorz&apos;jBX3 as follows. After abrB&apos; descrjjqq5 ofour simulation methodology in Sect. 2, weprFXz t modeling rling3 in Sect. 3, followed by a conclusion in Sect. 4.
Kettensteife Polymere – Struktur, Phasenverhalten und Eigenschaften (1989)

Abstract Die überwiegende Anzahl der heute in der Technik verwendeten Kunststoffe besteht aus flexiblen Makromolekülen, bei denen die aufeinanderfolgenden Grundeinheiten einen von 180° abweichenden Winkel bilden und gegeneinander verdrehbar sind. Kettensteife Polymere, die im Idealfall Stäbchengestalt aufweisen, sind bisher weit weniger gut untersucht worden. Der Grund hierfür liegt in der extremen Schwerlöslichkeit oder oft sogar der völligen Unlöslichkeit und Unschmelzbarkeit dieser Makromoleküle. In letzter Zeit haben aber gerade diese Materialien großes technisches Interesse gefunden, da sich daraus hochzugfeste Fasern und Formkörper herstellen lassen. Diese ungewöhnlichen mechanischen Eigenschaften leiten sich aus der nahezu parallelen Anordnung der stäbchenförmigen Kettenmoleküle im Festkörper ab, die durch Formgebung aus einer flüssigkristallinen (nematischen) Phase erhalten wird. Eine umfassende technische Nutzung dieser Materialien bedingt daher ein weitgehendes Verständnis des Phasenverhaltens und der Struktur der kettensteifen Polymere. Die Methodenvielfalt, die dem Polymerforscher heute zur Verfügung steht, hat dabei zu wichtigen Erkenntnissen geführt, die zusammen mit den Resultaten theoretischer Modelle zum Phasenverhalten direkt für die Praxis nutzbar gemacht werden können.
Functional Assessment of the Pharynx at Rest and during Swallowing in Partially Paralyzed Humans  (1997)

Vecuronium-induced partial paralysis cause pharyngeal dysfunction and increased risk for aspiration at mechanical adductor pollicis TOF ratios < 0.90. Pharyngeal function is not normalized until an adductor pollicis TOF ratio of > 0.90 is reached. The upper esophageal sphincter muscle is more sensitive to vecuronium than is the pharyngeal constrictor muscle.
An end-to-end approach to globally scalable programmable networking (2003)

The three fundamental resources underlying Information Technology are bandwidth, storage, and computation. The goal of wide area infrastructure is to provision these resources to enable applications within a community. The end-to-end principles provide a scalable approach to the architecture of the shared services on which these applications depend. As a prime example, IP and the Internet resulted from the application of these principles to bandwidth resources. A similar application to storage resources produced the Internet Backplane Protocol and Logistical Networking, which implements a scalable approach to wide area network storage. In this paper, we discuss the use of this paradigm for the design of a scalable service for wide area computation, or programmable networking. While it has usually been assumed that providing computational services in the network will violate the end-to-end principles, we show that this assumption does not hold. We illustrate the point by describing Logistical Network Computing, an extension to Logistical Networking that supports limited computation at intermediate nodes.
Nature and dynamic interpretation of deformation lamellae in calcite of three marbles (1953)

Dynamics of the Polish and Eastern Slovakian parts of the Carpathian accretionary wedge: insights from palaeostress analyses
The therapeutic potential of interleukin-6 hyperagonists and antagonists (1997)

Interleukin-6 (IL-6) is a 4-helical protein that binds to a specific IL-6 receptor on target cells and to two molecules of the promiscuous signal transducing protein, glycoprotein 130 (gp130). Structure-function analysis has led to the definition of molecular contacts between IL-6 and its receptor subunits. This knowledge has led to the design of competitive antagonistic proteins that retain their receptor binding capability, but fail to stimulate one or both gp130 proteins; the properties of such recombinant antagonistic proteins are compared with traditional neutralising monoclonal antibodies targeted at IL-6 or receptor subunits. Furthermore, several strategies have been employed to construct molecules with increased bioactivity. Possible therapeutic applications in putative IL-6 dependent haematologic disorders, e.g., Castleman's disease (CD), POEMS syndrome, multiple myeloma, and bone diseases, e.g., Paget's disease, osteoporosis, are outlined. IL-6 antagonists could also, in theory, suppress inflammatory activity in rheumatic and autoimmune diseases and could prevent secondary amyloidosis. This principle may prove advantageous in myocardial infarction (MI) and unstable angina pectoris. More generally, IL-6 antagonists could improve the wasting and microcytic anaemia of chronic diseases. IL-6 antagonists might slow down development of mesangio-proliferative glomerulonephritis (MPGN). Hyperagonistic variants of IL-6 have a potential use in the ex vivo expansion of haematopoietic progenitor cells and as thrombopoietic agents. They might well be the first drugs to aid liver regeneration in vivo.
Fluoroimmunoassays and immunofluorometric assays. (1985)

Fluorescent probes and fluorometric methods have gained increasing interest in the field of clinical immunology, not only as one additional alternative to radioimmunoassays, but also in producing cheap, stable, and safe reagents and rapid and sensitive assays. One of the main goals has been the development of homogeneous assays: assays based on fluorescence polarization, fluorescence quenching, excitation transfer, or enzymically releasable probes are widely applied, especially in drug monitoring. The development of suitable solid-phase separation techniques has facilitated utilization of fluorescence in heterogeneous assays, which in general have wider applications, from proteins and viruses to small haptens. Lately new alternative fluorescent probes and methods have been introduced. For example, the use of fluorescent phycobiliproteins or porphyrin derivatives with long-wavelength emission and large Stokes shift or, in particular, the rare earth chelates with unique fluorescent properties well suited to time-resolved measurement have opened new possibilities towards more sensitive immunoassays.
Determining Contents Power Users for Revitalizing Blog Networks (2009)

In a blog network, there are special users who induce other users to actively utilize blog services. In this paper, these users whose contents exhibit large influence over other bloggers are defined as 'Content Power Users' (CPUs). It is important to accurately determine who content power users are in a blog network in order to establish business policies that will stimulate usage of blog services. In this paper, we discuss a novel method of determining content power users. First, we propose a system of measuring the influence of content of each post owned by individual users. Then, by adjusting the measured values based on the time of exposure and adding them up, we calculate the power of influence for corresponding users. Finally, by applying the proposed method to actual blog networks and comparing the selected power users to those of a preexisting method, we analyze different methods of determining power users. The experimental results demonstrate that our method of determining power users reflects well dynamic changes in a blog network.
pKa shift-associated effects in enantioseparations by cyclodextrin-mediated capillary zone electrophoresis (1999)

Enantioselective migration of dansylated (Dns) amino acids in the presence of hydroxypropylated-beta-cyclodextrin under acidic conditions near the pI value of the analytes was investigated by means of capillary zone electrophoresis. Based on the migration data, the pH dependence of the complexation constants was evaluated, as well as the variation of the complex mobilities with pH. As a result of these data, the migration behavior in the pH region near the pI could be understood, which, in some instances, includes the reversal of migration order upon variation of selector concentration. The enantioselective pKa shifts upon complexation could be quantitated for the carboxylic and the amino group separately. pKa shifts were found in the order of 0.8 pI units, the differences between the enantiomers being up to 0.25 pH units. These data were in agreement with the pI shifts reported from isoelectric focusing experiments. The accurate determination of the pI values of the Dns amino acids makes it possible to calibrate the pI scale in isoelectric focusing in the presence of chiral selectors.
Isolation and Characterization of Two Novel Phosphodiesterase PDE11A Variants Showing Unique Structure and Tissue-specific Expression (2000)

cDNAs encoding a novel phosphodiesterase, phosphodiesterase 11A (PDE11A), were isolated by a combination of reverse transcriptase-polymerase chain reaction using degenerate oligonucleotide primers and rapid amplification of cDNA ends. Their catalytic domain was identical to that of PDE11A1 (490 amino acids) reported during the course of this study. However, the cDNAs we isolated had N termini distinct from PDE11A1, indicating two novel N-terminal variants of PDE11A. PDE11A3 cDNA encoded a 684-amino acid protein including one complete and one incomplete GAF domain in the N-terminal region. PDE11A4 was composed of 934 amino acids including two complete GAF domains and shared 630 C-terminal amino acids with PDE11A3 but had a distinct N terminus containing the putative phosphorylation sites for cAMP- and cGMP-dependent protein kinases. PDE11A3 transcripts were specifically expressed in testis, whereas PDE11A4 transcripts were particularly abundant in prostate. Recombinant PDE11A4 expressed in COS-7 cells hydrolyzed cAMP and cGMP with K(m) values of 3.0 and 1.4 microm, respectively, and the V(max) value with cAMP was almost twice that with cGMP. Although PDE11A3 showed the same K(m) values as PDE11A4, the relative V(max) values of PDE11A3 were approximately one-sixth of those of PDE11A4. PDE11A4, but not PDE11A3, was phosphorylated by both cAMP- and cGMP-dependent protein kinases in vitro. Thus, the PDE11A gene undergoes tissue-specific alternative splicing that generates structurally and functionally distinct gene products.
Kinetic Analysis of Four HIV-1 Reverse Transcriptase Enzymes Mutated in the Primer Grip Region of p66 (1997)

The highly conserved primer grip region in the p66 subunit of HIV-1 reverse transcriptase (RT) is formed by the beta12-beta13 hairpin (residues 227-235). It has been proposed to play a role in aligning the 3'-OH end of the primer in a position for nucleophilic attack on an incoming dNTP. To analyze the importance of the primer grip for RT function, mutant RTs were used that contain single alanine substitutions of residues Trp229, Met230, Gly231, and Tyr232 in the p66 subunit of the heterodimeric p66/51 enzyme. Steady-state and pre-steady-state kinetic analyses of the enzymes were performed. All mutant enzymes revealed reduced polymerase activity. Mutation of Y232A showed the smallest effect on polymerase function. Equilibrium fluorescence titrations demonstrated that the affinity of the mutants for tRNA was only slightly affected. However, the affinity for primer-template DNA was reduced 27-fold for mutant p66(W229A)/51 and 23-fold for mutant p66(G231A)/51, and the maximal pre-steady-state rate of nucleotide incorporation, kpol, was reduced 27-fold for p66(W229A)/51 and 70-fold for p66(G231A)/51, respectively. Mutant p66(M230A)/51 revealed no reduced affinity for primer-template but showed a 71-fold reduced affinity for dTTP. Additionally, the mutations Trp229 and Gly231 affected the stability of the RT heterodimer.
Inhibition of the Stromal p38MAPK/MK2 Pathway Limits Breast Cancer Metastases and Chemotherapy-Induced Bone Loss (2018)

The role of the stromal compartment in tumor progression is best illustrated in breast cancer bone metastases, where the stromal compartment supports tumor growth, albeit through poorly defined mechanisms. p38MAPKα is frequently expressed in tumor cells and surrounding stromal cells, and its expression levels correlate with poor prognosis. This observation led us to investigate whether inhibition of p38MAPKα could reduce breast cancer metastases in a clinically relevant model. Orally administered, small-molecule inhibitors of p38MAPKα or its downstream kinase MK2 each limited outgrowth of metastatic breast cancer cells in the bone and visceral organs. This effect was primarily mediated by inhibition of the p38MAPKα pathway within the stromal compartment. Beyond effectively limiting metastatic tumor growth, these inhibitors reduced tumor-associated and chemotherapy-induced bone loss, which is a devastating comorbidity that drastically affects quality of life for patients with cancer. These data underscore the vital role played by stromal-derived factors in tumor progression and identify the p38MAPK-MK2 pathway as a promising therapeutic target for metastatic disease and prevention of tumor-induced bone loss.<b>Significance:</b> Pharmacologically targeting the stromal p38MAPK-MK2 pathway limits metastatic breast cancer growth, preserves bone quality, and extends survival. <i>Cancer Res; 78(19); 5618-30. ©2018 AACR</i>.