gebhart/patrepeval huggingface dataset:
VEGF signalling: integration and multi-tasking in endothelial cell biology (2003)

The central role of VEGF (vascular endothelial growth factor A) in angiogenesis is dependent upon its ability to co-ordinately regulate multiple endothelial functions. The multifunctionality of VEGF at the cellular level results from its ability to initiate a diverse, complex and integrated network of signalling pathways via its major receptor, kinase-insert-domain-containing receptor (KDR). Activation of phospholipase C-gamma, protein kinase C, Ca(2+), ERK (extracellular-signal-regulated protein kinase), Akt, Src, focal adhesion kinase and calcineurin pathways has been implicated in mediating multiple VEGF functions, including survival, proliferation, migration, vascular permeability, tubulogenesis, NO and prostanoid synthesis, and gene expression. NO and prostanoids in turn play paracrine and autocrine roles in linking post-receptor signalling to biological functions. Integration between biologically important signalling cascades occurs at several points. Akt and ERK, for example, are key junction points linking together signal transduction involved in survival and NO generation, and proliferation and prostanoid biosynthesis. Together, the multiplicity, functional versatility and integration of VEGF signalling provide a useful framework for understanding the mechanisms underlying the endothelial biological response to this key factor.
Optimum conditions for generating channelling patterns in the scanning electron microscope (1969)

The optimum conditions are discussed for generating channelling patterns (i.e. orientation dependent lines and bands of contrast) in the scanning electron microscope. From considerations of the electron optics it is shown that patterns of highest angular resolution may be obtained by establishing a compromise between a small divergence in a collimated beam and a high beam current at the specimen surface. It is also shown that surface detail finer than 50 μm may be resolved and superimposed on channelling patterns with only small loss in pattern resolution. Finally, a technique is presented for obtaining selected area channelling patterns from areas smaller than 100 μm across.
Microprocessor-Controlled Digital Shunt Regulator (1980)

A new approach to the design of power systems is presented in which a microprocessor is used as a controller for a digital shunt regulator (DSR). This approach meets the demands of future space and ground missions, i. e., high efficiency, high reliability, low weight, low volume, increased flexibility, and less development time. This approach responds to future demands by permitting realtime modification of system parameters for system optimization. This feature is especially important in the event of an anomaly. As the microprocessor need not be dedicated to the DSR, it can simultaneously be used for battery management and for charge regulator/discharge regulator control. This approach also reduces the component count, simplifies assembly and testing of the unit, results in significant time saving, and increases the reliability.
Effect of Nitrate in the Rooting Medium on Carbohydrate Composition of Soybean Nodules (1981)

Nodulated soybean plants (Glycine max [L.] Merr) were grown in sand culture. Carbohydrate composition of nodules, roots, and leaf blades was determined and related to the effects of nitrate in nutrient solution on nodule growth and on nitrogenase activity of nodules.When plants were grown without nitrate for 6 weeks and then supplied with 150 milligrams NO(3)-N/liter for 4 or 7 days, there was an 80% decline in acetylene reduction activity of nodulated roots, relative to the 0-N control. The 80% decline in nitrogenase activity was accompanied by a decline in nodule glucose concentration of about 0.15 milligram per gram fresh weight and an increase in nodule sucrose concentration of about 0.23 milligram per gram fresh weight.Plants were grown with 0, 30, or 100 milligrams NO(3)-N/liter for 5 or 6 weeks to study long-term effects of nitrate on nodule growth. The 100-N treatment reduced nodule weight/plant by 70% but reduced the sum of sucrose + glucose + fructose concentration in nodules by only 12%. The ratios of [sucrose] in nodules/[sucrose] in roots and [fructose] in nodules/ [fructose] in roots increased slightly in response to nitrate, indicating that nitrate affects sugar concentration in roots more than sugar concentration in nodules.The effect of nitrate on glucose concentration of nodules was consistently negative. However, if it is assumed that sucrose, glucose, and fructose are equivalent in their ability to support nodule functions, then the overall results are not consistent with the hypothesis that nitrate inhibits nodule growth and activity by reducing the accumulation of carbohydrate in nodules.
Inhibition of Threonine Dehydratase Is Herbicidal (1994)

Threonine dehydratase, the first enzyme in isoleucine biosynthesis, catalyzes deamination and dehydration of threonine to produce 2-ketobutyrate and ammonia. An antimetabolite, 2-(1-cyclohexen-3(R)-yl)-S-glycine (CHG), inhibits the plant enzyme. CHG inhibits the growth of Black Mexican Sweet corn (Zea mays) cells and of Arabidopsis thaliana plants. The herbicidal effects of CHG can be reversed by 2-ketobutyrate, other intermediates of isoleucine biosynthesis, and by isoleucine itself. These results suggest that the herbicidal effects observed with CHG are a consequence of inhibition of threonine dehydratase. The enzyme could be a potential target site for an herbicide screening program.
Genetic regulation of self‐incompatibility (1993)

Abstract Self‐incompatibility is a cell‐cell recognition system in higher plants that is based on the ability of the pistil to discriminate “self‐pollen from “non‐self"‐pollen. In the simplest systems, this recognition response is controlled by a single locus — the S‐locus — with multiple alleles. Pollination of a pistil with pollen bearing an S‐allele recognition factor identical to that expressed in the host plant stigma or style results in rejection of the “self"‐pollen. Most of the studies on the molecular genetics of self‐incompatibility that are summarized in this review have had as their goal the identification and characterization of the gene product(s) associated with the self‐incompatibility response. These studies have provided a great deal of new and important information about self‐incompatibility — despite the fact that many critical questions remain unresolved. Taken together, the present evidence from these studies indicates that the self‐incompatibility response is likely to be far more complex than suggested by historical models.
International Semiconductor Device Research Symposium (ISDRS-91) Held in Charlottesville, Virginia on 4-6 December 1991 (1992)

The First International Semiconductor Device Research Symposium (ISDRS-91) took place in Charlottesville, Va on December 4-6, 1991 for the purpose of providing a convenient forum for the exchange of information and new ideas for researchers from industry, university, and government laboratories with leading researchers from the United States, Canada, Europe, Asia, and the former Soviet Union. As the first international conference of its kind to take place after the August 1991 coup attempt in the Soviet Union, it was unique with the presence of an unusually large contingent of Russian scientists. The emphasis of the program was on novel ideas--advanced semiconductor technologies still in their infancy whose tangible technological outcomes are not expected for another five to ten years. Some of the technologies discussed at the symposium included bandgap engineering, large area semiconductor electronics, new millimeter wave and optoelectronics technologies, and silicon carbide and diamond devices.
Infliximab for the Treatment of Posterior Uveitis with Retinal Neovascularization in Behçet Disease (2004)

Purpose To report a case of posterior uveitis with retinal neovascularization in a patient with Behçet disease treated with infliximab. Methods A 50-year-old man with a history of recurrent relapses of ocular inflammation despite immunosuppressive therapy developed retinal neovascularization near the optic disk. The patient was treated with infliximab and followed up for 12 months. Results Retinal neovascularization regressed 8 months after the first anti–tumor necrosis factor (TNF) treatment and with six infusions of infliximab. The ocular inflammation resolved almost completely. Conclusions The result suggests that anti-TNF therapy may be effective in the treatment of retinal neovascularization caused by panuveitis in Behçet disease.
Baseline Studies of the Clay Minerals Society Source Clays: Geological Origin (2001)

In any clay deposit, the nature of the mineral assemblage and the composition of individual clay minerals can change radically in a few em. Consequently, any given locality can contain many subtly different types of clay minerals. Results from different laboratories on ostensibly the same clay material may not always be comparable because the samples may indeed not contain an assembly of identical clay minerals. Such confusion slows the understanding of this important group of minerals. Several attempts were made to provide investigators with reasonably constant clay materials, the first being that of the American Petroleum Institute Project 49 (Kerr, 1949). The Clay Minerals Society Source Clays project proposed to provide investigators with gently homogenized clay materials, carefully collected and processed under the supervision of clay specialists. The collection would include metric ton amounts to ensure a long-lasting collection. The program began in 1972, with the introduction of the materials described in this paper. Later the program expanded to include the Special Clays. These samples are materials not amenable to homogenization, or they are available in very small amounts.
THE EFFECT OF PROLONGED CULTIVATION IN VITRO UPON THE PATHOGENICITY OF YELLOW FEVER VIRUS (1937)

1. Experimental evidence is presented to show that prolonged cultivation of yellow fever virus in vitro results in a change in its pathogenicity, and that this change varies with the type of tissues used for the cultivation. 2. In the tissue cultures used for the propagation of the virus, three different types of tissues were used. They included whole mouse embryo, chick embryo from which the head and spinal cord had been removed, and testicular tissues of mice and guinea pigs. 3. The changes in the pathogenicity of the virus cultivated for a period of over 3 years in a medium containing the tissues of whole mouse embryo were not striking. The viscerotropic virulence of the virus appeared somewhat diminished, in that when injected subcutaneously into rhesus monkeys or hedgehogs it failed to produce a fatal infection, although there is evidence to indicate that a generalized infection takes place as demonstrated by the appearance of virus in the circulating blood in relatively high concentration during infection. The neurotropic virulence of the virus remained unaltered during the cultivation in this medium. 4. The changes in the pathogenicity of the virus cultivated in medium containing tissues of chick embryo from which the head and spinal cord had been removed were very pronounced. The viscerotropic virulence of the virus was lost to a large extent. When injected subcutaneously into monkeys there was as a rule a very mild generalized infection, as demonstrated by the minimal quantities of virus found in the circulating blood. Its neurotropism was also much diminished. When injected into monkeys intracerebrally, it no longer produced a fatal encephalitis but only a moderate febrile reaction, followed by recovery and solid immunity to reinoculation with a highly virulent strain of virus. When injected intracerebrally into mice, the mortality ratio was not diminished but the incubation period was markedly prolonged. 5. The changes in the pathogenicity of the virus cultivated in medium containing testicular tissues were somewhat similar to those observed after cultivation in chick embryo medium which contained only a minimal amount of nervous tissue. Its viscerotropic affinity had been largely lost and only very small amounts of virus were found in the circulating blood of monkeys inoculated subcutaneously. Given intracerebrally, it produced death from encephalitis in monkeys. The incubation period in mice inoculated intracerebrally with this virus was also prolonged but somewhat less so than with the virus grown in chick embryo tissues without the central nervous system.
Strategic Combinations: The Future of Oncolytic Virotherapy with Reovirus (2016)

The dominant cancer treatment modalities such as chemotherapy, radiotherapy, and even targeted kinase inhibitors and mAbs are limited by low efficacy, toxicity, and treatment-resistant tumor subclones. Oncolytic viral therapy offers a novel therapeutic strategy that has the potential to dramatically improve clinical outcomes. Reovirus, a double-stranded benign human RNA virus, is a leading candidate for therapeutic development and currently in phase III trials. Reovirus selectively targets transformed cells with activated Ras signaling pathways; Ras genes are some of the most frequently mutated oncogenes in human cancer and it is estimated that at least 30% of all human tumors exhibit aberrant Ras signaling. By targeting Ras-activated cells, reovirus can directly lyse cancer cells, disrupt tumor immunosuppressive mechanisms, reestablish multicellular immune surveillance, and generate robust antitumor responses. Reovirus therapy is currently being tested in combination with radiotherapy, chemotherapy, immunotherapy, and surgery. In this review, we discuss the current successes of these combinatorial therapeutic strategies and emphasize the importance of prioritizing combination oncolytic viral therapy as reovirus-based treatments progress in clinical development. Mol Cancer Ther; 15(5); 767-73. ©2016 AACR.
Overlapping retrovirus U5 sequence elements are required for efficient integration and initiation of reverse transcription (1991)

A secondary structure in the 5' noncoding region of avian retrovirus RNA, called the U5-leader stem, was shown previously to have a role in initiation of reverse transcription (D. Cobrinik, L. Soskey, and J. Leis, J. Virol. 62:3622-3630, 1988). We now show that an additional RNA secondary structure near the U5 terminus, called the U5-IR stem, is also important for reverse transcription. Mutations that disrupt the U5-IR stem cause a replication defect associated with both a decrease in synthesis of viral DNA in infected cells and a decrease in initiation of reverse transcription in melittin-permeabilized virions. Structure-compensating base substitutions in the U5-IR restore reverse transcription efficiency. In viral DNA, U5-IR sequences are included in the U5 terminal region that functions as a viral integration donor site. When base substitutions are introduced into these sequences, a reduced efficiency of integration in vitro and in vivo is observed. These observations indicate that U5-IR sequences have a structural role in reverse transcription of viral RNA and a sequence-specific role in the integration of viral DNA.
Low temperature fired NiCuZn ferrite (1994)

Bismuth oxide (Bi/sub 2/O/sub 3/) and lead glass were introduced to NiCuZn ferrite to reduce the sintering temperatures from more than 950/spl deg/C to 875/spl deg/C for co-firing with the internal conductor, Ag. Both additives formed liquid phases in grain boundaries during the sintering process to enhance the densification of the specimens. The chip inductors made of the Bi/sub 2/O/sub 3/ doped ferrite exhibited higher DC resistance than the un-doped formulation due to the intensified Ag diffusion by the aforementioned liquid phase.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
National Institue of Advanced Industrial Science and Technology (AIST) (2003)

We propose a method of using clustering techniques for partitioning a set of orders, which are sequences of objects sorted according to some property, such as size, preference, or price. These orders are useful for, say, carrying out a sensory survey. We propose a method called the k-order means (k-o’means) method. We compared our method with the traditional clustering methods, and analyzed its characteristics. We also applied our method to questionnaire survey data on people’s preferences in types of sushi.
Evar and Complex Anatomy: An Update on Fenestrated and Branched Stent Grafts (2008)

Endovascular aneurysm repair (EVAR) offers a minimally invasive treatment to patients with improved short-term and similar mid-term results compared to conventional, open repair (OR). EVAR is preferred by patients due to the reduction of surgical trauma. Approximately 20% of patients have aneurysm neck morphology which is inadequate for a standard stent graft and requires the endograft to cross vital aortic side branches to achieve a seal. This chapter describes the evolution of three types of devices, namely the fenestrated and branched stent grafts as well as the chimney grafts. These stent grafts incorporate vital aortic side branches in the repair, thereby increasing the applicability of EVAR which may improve the overall results.
Cardiac Endothelial Cells Regulate Reactive Oxygen Species-induced Cardiomyocyte Apoptosis through Neuregulin-1β/erbB4 Signaling (2004)

Neuregulin (NRG)-1beta has a prosurvival effect on cardiac myocytes via the phosphatidylinositol-3-kinase/Akt pathway, but the physiological regulators of this system in the intact heart are unknown. In this study, we tested the hypothesis that reactive oxygen species regulate NRG/erbB signaling. We used isolated adult rat ventricular myocytes (ARVMs) or cardiac microvascular endothelial cells (CMECs) in monoculture, or together in coculture. H2O2 induced NRG-1beta release from CMECs in a concentration-dependent manner, and conditioned medium from H2O2-treated CMEC activated ARVM erbB4. NRG-1beta release occurred via proteolytic cleavage of 115-kDa transmembrane NRG-1beta and was inhibited by the metalloproteinase inhibitor 1,10-phenanthroline. In myocyte monoculture, H2O2 induced erbB4-dependent, but NRG-independent, activation of Akt. To elucidate the bioactivity of CMEC-derived NRG-1beta on ARVMs, we examined H2O2-induced myocyte apoptosis in co-culture using an antibody to NRG-1beta. The percentages of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells were significantly higher in the anti-NRG-1beta group than in the control group. The change in apoptosis induced by anti-NRG-1beta in co-culture was similar in magnitude to the protection of myocytes by addition of recombinant NRG-1beta to ARVM monocultures. Activation of NRG/erbB paracrine signaling was also seen in the intact heart subjected to oxidative stress by ischemia-reperfusion injury. Isolated perfused mouse hearts subjected to 15 min of ischemia, followed by 30 min of reperfusion, showed complete proteolytic cleavage of 115-kDa NRG-1beta, with concomitant erbB4 phosphorylation. These results demonstrate that reactive oxygen species activate NRG-1beta/erbB4 paracrine signaling in the heart and suggest that this system is involved in cardiac adaptation to oxidative stress.
Constraining specificity in the N‐domain of tissue inhibitor of metalloproteinases‐1; gelatinase‐selective inhibitors (2007)

The tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of the matrix metalloproteinases (MMPs). Since unregulated MMP activities are linked to arthritis, cancer, and atherosclerosis, TIMP variants that are selective inhibitors of disease-related MMPs have potential therapeutic value. The structures of TIMP/MMP complexes reveal that most interactions with the MMP involve the N-terminal pentapeptide of TIMP and the C-D beta-strand connector which occupy the primed and unprimed regions of the active site. The loop between beta-strands A and B forms a secondary interaction site for some MMPs, ranging from multiple contacts in the TIMP-2/membrane type-1 (MT1)-MMP complex to none in the TIMP-1/MMP-1 complex. TIMP-1 and its inhibitory domain, N-TIMP-1, are weak inhibitors of MT1-MMP; inhibition is not improved by grafting the longer AB loop from TIMP-2 into N-TIMP-1, but this change impairs binding to MMP-3 and MMP-7. Mutational studies with N-TIMP-1 suggest that its weak inhibition of MT1-MMP, as compared to other N-TIMPs, arises from multiple (>3) sequence differences in the interaction site. Substitutions for Thr2 of N-TIMP-1 strongly influence MMP selectivity; Arg and Gly, that generally reduce MMP affinity, have less effect on binding to MMP-9. When the Arg mutation is added to the N-TIMP-1(AB2) mutant, it produces a gelatinase-specific inhibitor with Ki values of 2.8 and 0.4 nM for MMP-2 and -9, respectively. Interestingly, the Gly mutant has a Ki of 2.1 nM for MMP-9 and >40 muM for MMP-2, indicating that engineered TIMPs can discriminate between MMPs in the same subfamily.
Modeling Reservation-Based Autonomous Intersection Control in VISSIM (2013)

The use of autonomous vehicles is attracting more and more attention as a promising approach to improving both highway safety and efficiency. Most previous studies on autonomous intersection management relied heavily on custom-built simulation tools to implement and evaluate their control algorithms, but the use of nonstandard simulation platforms makes the comparison of systems almost impossible. Furthermore, without support from standard simulation platforms, reliable and trustworthy simulation results are hard to obtain. In this context, this paper explores a way to model autonomous intersections through the use of VISSIM, a standard microscopic simulation platform. A reservation-based intersection control system named autonomous control of urban traffic (ACUTA) was introduced and implemented in VISSIM through the use of VISSIM's external driver model. The operational and safety performance characteristics of ACUTA were evaluated with VISSIM's easy-to-use evaluation tools. In comparison with the results obtained with optimized signalized control, significantly reduced delays, along with a higher intersection capacity and lower volume-to-capacity ratios under various traffic demand conditions, resulted from the use of ACUTA. The safety performance of ACUTA was evaluated by use of the surrogate safety measure model, and few conflicts between vehicles within the intersection were detected. Moreover, the key steps and elements for implementation of ACUTA in VISSIM were introduced. These steps and elements can be useful for other researchers and practitioners implementing their autonomous intersection control algorithms in a standard simulation platform. By use of a standard simulation platform, the performance characteristics of autonomous intersection control algorithms can eventually be compared.
Activation of boar proacrosin is effected by processing at both N‐and C‐terminal portions of the zymogen molecule (1989)

A mixture of 55 and 53 kDa boar proacrosins was autoactivated at pH 8.5 to produce a 43 kDa intermediate form and a 35 kDa mature acrosin, and each of four forms of (pro)acrosins was isolated. Analysis of the N-terminal sequences of the two proacrosins indicated the existence of a segment corresponding to the acrosin light chain at the N-terminal end of the zymogen. Two N-terminal sequences identical with those of the light and heavy chains were found in the intermediate form and mature acrosin. The proacrosins and the intermediate contained many more proline residues than the mature enzyme. These results indicate that the activation of boar acrosin zymogen is achieved by the removal of a C-terminal segment rich in proline residues and by the cleavage of the Arg23-Val24 bond leading to the formation of the light and heavy chains.