gebhart/patrepeval huggingface dataset:
Reliability of Embedded Ultrathin Chips Subjected to Cyclic Stresses (2012)

Recently, ultra-thin embedded die technology has garnered much attention for its ability to produce assemblies in two or three dimensions with reduced footprint, low profile, low weight, and conformity. The embedded die technology can be successfully utilized in the development of flexible printed circuits to satisfy the demand for reliable and high density packaging solutions. With tremendous application potential in wearable and disposable electronics, the reliability of the flexible embedded die package is of paramount importance. Presented are results from the experimental study on the electrical performance of ultrathin (≤50 μm) silicon dice embedded into a polymer substrate and bumped using three different techniques—electroplating, wire bonding, and sputtering. The reliability of different packages was evaluated through a series of thermal and mechanical cyclic stress tests. The results suggested that the reliability of all embedded dice was high regardless of the bumping technology. However, compared with the other two methods, wire bonded stud bumps provided better and more consistent performance.
Use of a novel triple-tracer approach to assess postprandial glucose metabolism (2003)

Numerous studies have used the dual-tracer method to assess postprandial glucose metabolism. The present experiments were undertaken to determine whether the marked tracer nonsteady state that occurs with the dual-tracer approach after food ingestion introduces error when it is used to simultaneously measure both meal glucose appearance (R(a meal)) and endogenous glucose production (EGP). To do so, a novel triple-tracer approach was designed: 12 subjects ingested a mixed meal containing [1-(13)C]glucose while [6-(3)H]glucose and [6,6-(2)H(2)]glucose were infused intravenously in patterns that minimized the change in the plasma ratios of [6-(3)H]glucose to [1-(13)C]glucose and of [6,6-(2)H(2)]glucose to endogenous glucose, respectively. R(a meal) and EGP measured with this approach were essentially model independent, since non-steady-state error was minimized by the protocol. Initial splanchnic glucose extraction (ISE) was 12.9% +/- 3.4%, and suppression of EGP (EGPS) was 40.3% +/- 4.1%. In contrast, when calculated with the dual-tracer one-compartment model, ISE was higher (P < 0.05) and EGPS was lower (P < 0.005) than observed with the triple-tracer approach. These errors could only be prevented by using time-varying volumes different for R(a meal) and EGP. Analysis of the dual-tracer data with a two-compartment model reduced but did not totally avoid the problems associated with marked postprandial changes in the tracer-to-tracee ratios. We conclude that results from previous studies that have used the dual-tracer one-compartment model to measure postprandial carbohydrate metabolism need to be reevaluated and that the triple-tracer technique may provide a useful approach for doing so.
Friction based locomotion module for mobile MEMS robots (2007)

The "modulated friction inertial drive (MFID) principle" is presented as a locomotion solution for mm- size micro robots dealing with both size constraints and limitations in terms of power consumption. Based on one of the configurations of this locomotion principle a locomotion module (10 times 10 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) with two degrees of freedom (X, thetas <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">z</sub> ) has been developed. It consists of two horizontally vibrating masses actuated by electrostatic comb drives and fabricated by DRIE on a SOI wafer. The design as well as calculated and measured actuator displacements and resonance frequencies are presented. First locomotion experiments have shown a linear velocity of 0.207 mm/s for a driving voltage of 30 V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">p2p</sub> and a power consumption of only 16.6 muW.
Simple Model for Internal Photoemission (1971)

We have studied the influence of film thickness and collision processes upon the internal photoemission process in metal-semiconductor systems. A simple, one-dimensional random walk model has been used. The analysis shows that significantly larger yields may be expected for film thicknesses of the order of or less than electron-phonon mean free paths. The influence of energy dependent mean free paths has also been studied. The results are in qualitative agreement with the results of Fermi-age theory, but are somewhat different quantitatively.
Suppression of Soybean Yield Potential in the Continental United States by Plant Diseases from 2006 to 2009 (2010)

Research must focus on management of diseases that cause extensive losses, especially when funds for research are limited. Knowledge of the losses caused by various soybean diseases is essential when prioritizing research budgets. The objective of this project was to compile estimates of soybean yield potential losses caused by diseases for each soybean producing state in the United States from 2006 to 2009. This data is of special interest since the 4-year period summarized in this report, permits an examination of the impact of soybean rust that was first reported in the United States in 2004. Thus, in addition to the goal of providing this information to aid funding agencies and scientists in prioritizing research objectives and budgets, an examination of the impact of soybean rust on soybean yield losses relative to other diseases is warranted. Yield losses caused by individual diseases varied among states and years. Soybean cyst nematode caused more yield losses than any other disease during 2006 to 2009. Seedling diseases, Phytophthora root and stem rot, sudden death syndrome, Sclerotinia stem rot, and charcoal rot ranked in the top six of diseases that caused yield loss during these years. Soybean yield losses due to soybean rust and Sclerotinia stem rot varied greatly over years, especially when compared to other diseases. Accepted for publication 21 October 2010. Published 22 November 2010.
Ring transformation of oxazoles to fused imidazoles. New synthetic route for 6-methyl-2,3-diphenyl-7,8-dihydroimidazo[1,2-b]pyridazine and 5-methyl-2,3-diphenyl-6,7-dihydro-5H-pyrrolo[1,2-a]imidazole, and their perhydrobenzo analogues (1983)

Fused imidazoles were synthesized by an intramolecular ring transformation of γ-keto-oxazoles with hydrazine and by an intramolecular dehydration of γ-amino-oxazoles. γ-Keto-oxazoles (4) and (6) were prepared by the reaction of lithiated 2-methyl-4,5-diphenyloxazole (1) with the methyl enol ether of an α-bromo ketone, followed by hydrolysis. γ-Keto-oxazoles (4) and (6) gave the 7,8-dihydroimidazo[1,2-b]pyridazine (7) and the 6,7,8,9,9a, 10-hexahydroimidazo[1,2-b]cinnoline (8), respectively, on treatment with hydrazine hydrate in acetic acid. The transformed fused imidazoles (7) and (8) were further converted into the corresponding tetrahydroimidazo[1,2-b]pyridazine (9) and octahydroimidazo[1,2-b]cinnoline (10), respectively, by reduction with NaBH4. γ-Amino-oxazoles (11) and (12) were prepared from (4) and (6), respectively, on reduction with NaBH3CN in the presence of ammonium acetate. The pyrolysis of (11) and (12) provided the cyclodehydrated 6,7-dihydro-5H-pyrrolo[1,2-a]imidazole (13) and 5,6,7,8,8a,9-hexahydro-4aH-imidazo[1,2-a]indole (14), respectively.
Glypican-3 Expression Distinguishes Small Hepatocellular Carcinomas From Cirrhosis, Dysplastic Nodules, and Focal Nodular Hyperplasia-like Nodules (2006)

Distinguishing small hepatocellular carcinoma (HCC) from other types of small focal lesions that occur in a cirrhotic liver can be difficult on the basis of morphologic features alone. We investigated whether the expression of glypican-3 (GPC3) could be an ancillary tool in the histopathologic diagnostic process. We performed immunohistochemistry for GPC3 on 16 low-grade dysplastic nodules, 33 high-grade dysplastic nodules, 13 focal nodular hyperplasia-like nodules, and 59 HCCs with a diameter less or equal to 3 cm present in the cirrhotic liver of 66 patients. Both resected lesions and lesions biopsied by needle were included and nonlesional cirrhotic parenchyma was also stained. In a subset of cases (23 samples of cirrhosis, 4 low-grade dysplastic nodules, 5 high-grade dysplastic nodules, 2 focal nodular hyperplasia-like nodules, and 18 HCCs), real time reverse transcriptase-polymerase chain reaction for GPC3 was performed. GPC3 expression was, both on immunohistochemistry and by real time reverse transcriptase-polymerase chain reaction, much higher in small HCCs than in cirrhosis and other types of small focal lesions, indicating that the transition from premalignant lesions to small HCC is associated with a sharp increase of GPC3 expression in a majority of cases. The sensitivity and specificity of a positive GPC3-staining for the diagnosis of HCC in small focal lesions was 0.77 and 0.96, respectively, in resected cases, and 0.83 and 1, respectively, for needle biopsies. Because the result of the staining was easily interpretable, immunohistochemistry for GPC3 is valuable ancillary tool in the histopathologic diagnosis of small focal lesions in cirrhosis.
Two Plasmodium Rhomboid Proteases Preferentially Cleave Different Adhesins Implicated in All Invasive Stages of Malaria (2006)

Invasion of host cells by the malaria pathogen Plasmodium relies on parasite transmembrane adhesins that engage host-cell receptors. Adhesins must be released by cleavage before the parasite can enter the cell, but the processing enzymes have remained elusive. Recent work indicates that the Toxoplasma rhomboid intramembrane protease TgROM5 catalyzes this essential cleavage. However, Plasmodium does not encode a direct TgROM5 homolog. We examined processing of the 14 Plasmodium falciparum adhesins currently thought to be involved in invasion by both model and Plasmodium rhomboid proteases in a heterologous assay. While most adhesins contain aromatic transmembrane residues and could not be cleaved by nonparasite rhomboid proteins, including Drosophila Rhomboid-1, Plasmodium falciparum rhomboid protein (PfROM)4 (PFE0340c) was able to process these adhesins efficiently and displayed novel substrate specificity. Conversely, PfROM1 (PF11_0150) shared specificity with rhomboid proteases from other organisms and was the only PfROM able to cleave apical membrane antigen 1 (AMA1). PfROM 1 and/or 4 was thus able to cleave diverse adhesins including TRAP, CTRP, MTRAP, PFF0800c, EBA-175, BAEBL, JESEBL, MAEBL, AMA1, Rh1, Rh2a, Rh2b, and Rh4, but not PTRAMP, and cleavage relied on the adhesin transmembrane domains. Swapping transmembrane regions between BAEBL and AMA1 switched the relative preferences of PfROMs 1 and 4 for these two substrates. Our analysis indicates that PfROMs 1 and 4 function with different substrate specificities that together constitute the specificity of TgROM5 to cleave diverse adhesins. This is the first enzymatic analysis of Plasmodium rhomboid proteases and suggests an involvement of PfROMs in all invasive stages of the malaria lifecycle, in both the vertebrate host and the mosquito vector.
Photo-responsive bio-inspired adhesives: facile control of adhesion strength via a photocleavable crosslinker (2017)

A photo-responsive bio-inspired terpolymer adhesives consisting of a zwitterionic polymer, catechol moiety, and nitrobenzyl crosslinker was synthesized for convenient control of adhesion strength under UV irradiation.
Inducible ablation of astrocytes shows that these cells are required for neuronal survival in the adult brain (2001)

To study the function of astrocytes in the adult brain, we have targeted the expression of E. coli nitroreductase (NTR) to the astrocytes of transgenic mice under the control of the GFAP promoter. The astrocytes expressing NTR were selectively ablated after administration of the prodrug CB1954, resulting in motor discoordination. Histological examination showed that the region most affected in the brain was the cerebellum, in which the Bergmann glia were eliminated and the granular neurons had degenerated. Specific effects were also noted on the dendrites of the Purkinje cells, and the junction between these neurons and granular layer was disrupted. Astrocyte ablation was associated with a dramatic decrease in the expression of glutamate transporters, which may account for the degeneration of granular neurons since the excitotoxic effects of glutamate result in a similar phenotype. These results provide the first evidence that astrocytes are important for the survival of neurons in the adult brain in vivo.
Multiple Constraints at the Level of TCRα Rearrangement Impact Vα14i NKT Cell Development (2007)

CD1d-restricted NKT cells that express an invariant Valpha14 TCR represent a subset of T cells implicated in the regulation of several immune responses, including autoimmunity, infectious disease, and cancer. Proper rearrangement of Valpha14 with the Jalpha18 gene segment in immature thymocytes is a prerequisite to the production of a TCR that can be subsequently positively selected by CD1d/self-ligand complexes in the thymus and gives rise to the NKT cell population. We show here that Valpha14 to Jalpha rearrangements are temporally regulated during ontogeny providing a molecular explanation to their late appearance in the thymus. Using mice deficient for the transcription factor RORgamma and the germline promoters T early-alpha and Jalpha49, we show that developmental constraints on both Valpha and Jalpha usage impact NKT cell development. Finally, we demonstrate that rearrangements using Valpha14 and Jalpha18 occur normally in the absence of FynT, arguing that the effect of FynT on NKT cell development occurs subsequent to alpha-chain rearrangement. Altogether, this study provides evidence that there is no directed rearrangement of Valpha14 to Jalpha18 segments and supports the instructive selection model for NKT cell selection.
Characterization of a Monoclonal Antibody B1 That Recognizes Phosphorylated Ser-158 in the Activation Peptide Region of Human Coagulation Factor IX (2006)

Blood coagulation factor IX (FIX) undergoes various post-translational modifications such as gamma-carboxylation and glycosylation. Non-phosphorylated recombinant FIX has been reported to rapidly disappear from plasma, indicating that phosphorylation of FIX plays an important role in the physiological activity of this coagulation factor. In this study, we characterized the human FIX activation peptide (AP) using a monoclonal antibody that recognizes phosphorylated Ser-158 in the AP region. Murine monoclonal antibody B1 against human FIX recognized FIX with an apparent K(d) value of 5 nm in the presence of Ca(2+) (EC(50) = 0.58 mm). B1 bound to the isolated AP of FIX and retained the Ca(2+) dependence of binding to the isolated AP. The deglycosylation of AP did not affect the binding of B1 to AP, while B1 failed to bind to recombinant AP expressed in Escherichia coli. MALDI-TOF mass spectrometry showed that the m/z of plasma-derived deglycosylated AP is 82.54 Da greater than that of recombinant AP. The binding ability of B1 to AP was lost by the dephosphorylation of plasma-derived AP. B1 bound to synthetic peptide AP-(5-19), including phosphoserine-13, but not to the non-phosphorylated AP-(5-19) in the presence of Ca(2+). These data provide direct evidence that Ser-13 of the plasma-derived FIX AP region (Ser-158 of FIX) is phosphorylated and that B1 recognizes the epitope, which includes Ca(2+)-bound phosphoserine-158. B1 should be useful in the quality control of biologically active recombinant FIX containing phosphoserine-158.
Diabetic hypoglycemia (1985)

Hypoglycaemia is quantitatively by far the commonest acute complication of diabetes mellitus (DM). A recent survey from Nottingham estimated that 9% of insulin-dependent (IDDM, Type 1) diabetics were treated in casualty for severe hypoglycaemia in a 12-month period. A similar 8-month prospective study in London gave a figure of 10%. Both these figures can be regarded as underestimates, as they do not include hypoglycaemia treated outside hospital by patient, friend, relative or general prac-
Laser communication component technologies: database, status, and trends (1996)

A database of component and subsystem technologies for free-space laser communications has been compiled. This document discusses technology assessment for free-space lasercomm components, and contains a collection of characteristics of commercially available and one-of- a-kind components that were made for laser communication and other relevant applications. The document also includes quantitative data on laser communication systems that were constructed in the past, along with plots of development trends for specific component technologies as a function of time. We intend to continually update this information with the assistance of lasercomm community and industries involved. First draft of the document will be distributed by mid 1996 for comments and corrections. It is expected that this collection of data will serve as a handbook to lasercomm system engineers and designers.
System ISI calibration: a universally applicable scheme is possible only when coumarin plasma calibrants are used (1997)

Coagulometer-induced errors in International Normalized Ratios (INR) may be corrected by assigning an International Sensitivity Index (ISI) specific for the local combination of coagulometer and thromboplastin reagent (System ISI). In the present study, 57 laboratories determined the INR of eight frozen coumarin plasmas. There was considerable variation in INR reported at the different centres, with coefficients of variation (CV) ranging from 11.30% to 17.29%. In addition, their consensus mean INR values were consistently higher than those obtained manually. The overall mean percentage deviation was 20.06%, indicating that correction was required to reduce the over-estimation of INR that occurred even in those Systems incorporating a thromboplastin reagent with an instrument-specific ISI. Local correction using coumarin plasma calibrants reduced this value to < or = 7% with current reference thromboplastins BCT 441, OBT 79 and RBT 90 and also lowered the CV to < 5%. In contrast, when adsorbed plasma calibrants were employed, mean percentage deviations were 7.87% with BCT 441, 12.47% with OBT 79 and 77.37% with RBT 90 as reference respectively. This difference may be due, in part, to the difficulty in assigning an INR to adsorbed plasma calibrants, which contain no protein induced by vitamin K antagonists (PIVKA). This study demonstrates that coumarin plasma calibrants are superior in System ISI calibration.
Generation of a 50,000-member human DNA library with an average DNA insert size of 75-100 kbp in a bacteriophage P1 cloning vector. (1990)

A bacteriophage P1 cloning system that permits the isolation and amplification of cloned DNA fragments as large as 100 kbp was described previously. We have now utilized a similar system to generate a 50,000-member human DNA library with DNA inserts ranging in size from 75 to 100 kbp. Two major obstacles were overcome in constructing the library. The first concerned the mcrAB restriction system of Escherichia coli, which degrades DNA containing MeC and interferes with the recovery of cloned human DNA inserts. In the P1 cloning system, the effect of the Mcr restriction activity is to decrease recovery of cloned inserts by about 35-fold when the activity is in the host cell line and by about 3-fold when the activity is in the cells used to prepare the packaging extract. To circumvent this problem we inactivated, by mutation, the McrAB proteins in both components of the cloning system. The second obstacle concerned the preferential cloning of small DNA fragments from a population of fragments ranging in size from 20 to 100 kbp. To deal with this problem we first modified the P1 lysogen used to prepare the in vitro head-tail packaging extract so that it would produce 12 times as many large P1 heads (head capacity about 110 kbp) as small P1 heads (head capacity about 45 kbp). We then restructured the P1 cloning vector so that it could be used to produce vector "arm" fragments that could be ligated to insert DNA at only one end. This prevented the formation of long concatamers consisting of alternating units of vector and insert DNA and prohibited the packaging of small inserts in large phage heads. Using the insert-biased large head extract, the arms vector, and size-selected human DNA fragments, we showed that as much as 90% of recovered transformants contained inserts in the desired high molecular weight range.
The Reincarnation of Virtual Machines (2004)

The term virtual machine initially described a 1960s operating system concept: a software abstraction with the looks of a computer system’s hardware (real machine). Forty years later, the term encompasses a large range of abstractions?for example, Java virtual machines that don’t match an existing real machine. Despite the variations, in all definitions the virtual machine is a target for a programmer or compilation system. In other words, software is written to run on the virtual machine.
Long‐Term Ethanol Consumption Selectively Impairs Ganglioside Pathway in Rat Brain (1998)

Gangliosides are sialo-glycosphingolipids that play important roles in the interaction of cells with their environment and are thus involved in the regulation of many cellular events. Sialic acid residues are important for the conformation of a glycomolecule, their structural stability and their functions. Although decreased brain ganglioside sialic acid has been previously reported as a result of chronic ethanol treatment in rats, no reports are available on the sialylation of specific gangliosides and/or the mechanism leading to depletion of their sialic acid residues. Therefore, in this investigation, we have examined the effects of chronic ethanol treatment on (1) incorporation of [4,5-3H]N-acetylmannosamine (ManNAc) into specific rat brain gangliosides, GD3, GD1a, GT1a, and GT1b; and (2) enzymatic activities of brain sialyltransferase and sialidase at specific subcellular levels. The experiments were done in male Wistar rats pair-fed with either ethanol or control liquid diets for a period of 8 weeks. The rats were intracerebroventricularly injected with labeled ManNAc (30 microCi/rat) and killed after 90 min. Radioactivity was determined in respective ganglioside bands separated on a thin layer chromatography system. Specific activities of sialyltransferase and sialidase were assessed using GM3 and GD3 as substrates, respectively. The results showed significant decreases of 57.7% (p < 0.001) and 68.9% (p < 0.001), respectively, in the labeled ManNAc incorporation into GD3 and GD1a fractions in rats of the ethanol group, compared with rats of the control group. No significant changes were noted in the incorporation of labeled ManNAc into GT1a or GT1b ganglioside fractions between the ethanol and control groups. Concomitantly, compared with control rats, a decrease of 18.9% (p < 0.05), 20.6% (p < 0.05), and 15.8% (p < 0.001) was found in the sialyltransferase activity, respectively, at the whole brain, and brain Golgi and synaptosomal levels. However, dramatic increases of 32.4% (p < 0.05), 105% (p < 0.001), and 150% (p < 0.001) in sialidase activity were found, respectively, at the whole brain and brain cytosol and synaptosomal fractions of rat treated chronically with ethanol. Thus, we conclude that the deleterious actions of ethanol on the sialylation of rat brain gangliosides is specific, and the reduced sialic acid label found in GD3 and GD1a in this study is mainly due to increased activity of brain sialidase. Furthermore, the study reaffirms our tenet that, regardless of whether it is the liver or the brain, glycosylation cascade is one of the main target of the deleterious attacks of ethanol.