gebhart/patrepeval huggingface dataset:
A PhoP-repressed gene promotes Salmonella typhimurium invasion of epithelial cells (1993)

The Salmonella typhimurium transcriptional regulators, PhoP/PhoQ, induce phoP-activated gene (pag) expression to promote virulence and intracellular survival within macrophages. This response to the macrophage intracellular environment is simulated by phoP/phoQ constitutive mutations (phenotype PhoPc) that increase the expression of pag genes and repress the synthesis of approximately 20 proteins encoded by phoP-repressed genes (prg genes) (S. I. Miller and J. J. Mekalanos, J. Bacteriol. 172:2485-2490, 1990). PhoPc bacteria are attenuated for mouse virulence, suggesting that prg genes are virulence genes. We now report the identification of five unlinked prg loci by use of the transposon TnphoA. In general, medium conditions (i.e., starvation) that activate pag expression repress prg expression. However, variable effects on the PhoP regulon were observed when bacteria were grown under different oxygen tensions (pag and prg genes) or exposed to low pH (prg genes), suggesting heterogenous control of the regulon. One prg locus, prgH, was demonstrated to contribute to mouse virulence by both the oral and the intraperitoneal routes. prgH was located at 59 min on the Salmonella chromosome, a region where other genes essential to invasion of epithelial cells are clustered. The prgH locus was highly linked to one invasion locus, hil (C.A. Lee, B.D. Jones, and S. Falkow, Proc. Natl. Acad. Sci. USA 89:1847-1851, 1992), although transcription of prgH was opposite that of the Tn5B50-encoded promoters that result in a hyperinvasive or hil phenotype. Both PrgH and PhoPc mutant S. typhimurium were found to be defective in induction of endocytosis by Madin-Darby canine kidney (MDCK) epithelial cells. The invasion defect of PrgH but not that of PhoPc mutant bacteria was complemented by plasmids containing prgH (hil) DNA. Therefore, two virulence properties of Salmonella species, induction of endocytosis by epithelial cells and survival within macrophages, are oppositely modulated by the PhoP/PhoQ virulence regulators.
Mechanisms for the Clinical Benefits of Angiotensin II Receptor Blockers (2005)

The renin-angiotensin-aldosterone system (RAAS) regulates sodium balance, fluid volume, and blood pressure (BP). It is also implicated in the progression of heart failure, hypertension, and kidney disease; this is mediated by the binding of angiotensin II (ang II) to the ang II type 1 but not the ang II type 2 receptor. Preclinical and clinical studies have shown that blockade of the RAAS with ang II receptor blockers (ARB) is effective not only in controlling BP but also in preventing end-organ damage. Through their mechanism of action, ARB may offer benefit with respect to endothelial dysfunction and vascular remodeling, as well as cardiac and renal protection. In addition, both recent and ongoing clinical trials help to clarify further the mechanisms for the benefits of using ARB across the cardiovascular continuum.
Advanced resin systems and 3D textile preforms for low cost composite structures (1993)

Advanced resin systems and 3D textile preforms are being evaluated at Lockheed Aeronautical Systems Company (LASC) under NASA's Advanced Composites Technology (ACT) Program. This work is aimed towards the development of low-cost, damage-tolerant composite fuselage structures. Resin systems for resin transfer molding and powder epoxy towpreg materials are being evaluated for processability, performance and cost. Three developmental epoxy resin systems for resin transfer molding (RTM) and three resin systems for powder towpregging are being investigated. Various 3D textile preform architectures using advanced weaving and braiding processes are also being evaluated. Trials are being conducted with powdered towpreg, in 2D weaving and 3D braiding processes for their textile processability and their potential for fabrication in 'net shape' fuselage structures. The progress in advanced resin screening and textile preform development is reviewed here.
The dual PPAR<i>α</i>/<i>γ</i> agonist, ragaglitazar, improves insulin sensitivity and metabolic profile equally with pioglitazone in diabetic and dietary obese ZDF rats (2005)

In 6- and 10-week-old obesity-prone (fa/fa) Zucker diabetic fatty (ZDF) rats, effects of prevention and intervention therapies, respectively, were compared between PPARalpha/gamma agonist, ragaglitazar (RAGA) and separate PPARgamma and alpha agonists, pioglitazone (PIO) and bezafibrate (BF). In a separate study, lean (+/+) ZDF rats fed highly palatable chow to induce dietary obesity and insulin resistance were treated similarly. To test insulin-secretory capacity, all animals underwent a hyperglycaemic clamp. Insulin sensitivity was improved equally by RAGA and PIO in fa/fa rats subjected to both prevention and intervention treatments (e.g., prevention HOMA-IR: -71 and -72%, respectively), as was hyperglycaemia (both -68%). BF had no effect on either parameter in any study. Plasma lipids were markedly reduced (by 48-77%) by RAGA in all studies, equivalent to PIO, but to a greater extent than BF. RAGA improved beta-cell function (HOMA-beta) more than three-fold with prevention and intervention therapies, whereas PIO showed improvement only in intervention therapy. Consistent with improved insulin sensitivity, glucose infusion rate during the clamp was 60% higher in RAGA-treated animals subjected to prevention therapy, but there was little additional insulin-secretory response, suggesting that insulin secretion was already maximal.Thus, RAGA and PIO equally improve metabolic profile in ZDF rats, particularly when administered early in the course of diabetes. They also improve beta-cell function, although this is better demonstrated through indices incorporating fasting insulin and glucose concentrations than through the hyperglycaemic clamp technique in this model.
Predominance of functional multidrug resistance (MDR-1) phenotype in CD34+ acute myeloid leukemia cells (1993)

The expression of the MDR-1-encoded P-170 glycoprotein (P-170) associated with clinical multidrug resistance (MDR) was investigated in 52 consecutive patients with untreated acute myeloid leukemia (AML). P- 170 expression was analyzed in correlation with CD34 expression and clinical response. Thirty of 52 patients expressed P-170 (58%). Eight of 30 P-170+ as compared with 16 of 22 P-170- patients achieved a complete remission (CR) (27% v 73%, P = .003). In 21 of 30 P-170+ patients, expression of the CD34 antigen was observed in greater than 10% of the blast cells, as compared with 14 of 22 P-170- patients (70% v 64%, P > .05). The CR rate of CD34+ and CD34- patients was 31% and 76%, respectively (P = .006). In AMLs that simultaneously expressed both P-170 and CD34, the CR rate was worse as compared with those negative for P-170 and CD34 (5% v 63%, P = .004). In 12 patients (8 P- 170+, 4 P-170-) CD34 and P-170 expression were further characterized by double fluorescence studies. It was shown that P-170+ cells were largely, but not exclusively, restricted to the CD34+ cell population. For the 8 P-170+ AML samples, the median ratio of P-170+/P-170- in CD34+ cells was 4.845 (range, 0.60 to 25.00) as compared with 0.135 (range, 0.02 to 0.67) in CD34- cells. In these 12 AML samples, the presence of functional resistance as defined by reduced daunorubicin accumulation was evaluated in CD34+ and CD34- AML cells. In 8 of 8 P- 170+ patients, intracellular daunorubicin accumulation in CD34+ AML blast cells was lower than in CD34- cells, and it increased after cyclosporin addition. No difference of intracellular daunorubicin accumulation was observed between CD34+ and CD34- AML cells of 4 P-170- patients. These data indicate that P-170 expression in AML with a heterogeneous CD34+ phenotype seems predominantly present in CD34+ AML blast cells.
GPS L1-L2 Bias Determination (1993)

Abstract : The GPS satellite L1-L2 bias T sub gd is determined using data from a TI-4100 receiver operating at Millstone Hill in Westford, MA. Pseudorange L1-L2 differences are computed. This difference is a measure of the total electron content (TEC) path delay along the line of sight plus the L1-L2 satellite and receiver bias. The difference of two measurements at the same time and satellite position cancels the TEC path delay and possible receiver bias and is a measure of the difference in satellite bias of the two satellites; i.e., Sv(a)L1-L2 - Sv(b)L1-L2. such direct measurements are not often available. Therefore, measurements at the same time are converted to 'zenith' TEC values using a mapping function. These difference are used in a least squares solution to determine the difference in bias between GPS satellites. Individual bias differences are obtained with an accuracy of 0.1 ns (o.285 TEC units) or better. By choosing one satellite as the reference, the individual satellite biases can also be obtained. The stability of each satellite bias is also estimated by examining a time series of the differences.
Dual latching solenoid valve Patent (1968)

Valve assembly for controlling simultaneously more than one fluid flow, and having stable qualities under loads
Stimulation of Short-term Plant Growth by Glycerol Applied as Foliar Sprays and Drenches under Greenhouse Conditions (2011)

The effects of aqueous solutions applied as foliar spray and drench applications of glycerol were tested on the ‘Chantenay’ carrot ( Daucus carota L.) family Apiaceae, corn ( Zea mays L.) family Poaceae, and spearmint ( Mentha spicata L.) family Lamiaceae under greenhouse conditions. Foliar sprays and drenches were administered to carrots at concentrations of 0, 1, 3, 5, 10, 25, or 50 ml·L −1 . Fresh weights, dry weights, and taproot diameter from carrot seedlings sprayed with a solution containing 5 mL·L −1 (50 m m ) glycerol increased 105.6%, 158.4%, and 53.8%, respectively, when compared with untreated carrots. Foliar sprays were administered to corn at concentrations of 0, 0.1, 0.3, 0.5, and 1 ml·L −1 and spearmint at concentrations of 0, 1, 5, and 10 mL·L −1 . Growth responses increased in corn and spearmint by using certain glycerol concentrations. Fresh weights, dry weights, and shoot length from corn seedlings sprayed with a solution containing 0.5 mL·L −1 (5 m m ) glycerol increased 83.5%, 154.6%, and 90.9%, respectively, when compared with untreated corn. Fresh weights, dry weights, and shoot length from mint plants sprayed with a solution containing 5 mL·L −1 (50 m m ) glycerol increased 46.6%, 68.7%, and 102.5%, respectively, when compared with untreated plants. Glycerol applications can stimulate growth responses in diverse plant species.
Heuristic evaluation of virtual reality applications (2004)

Journal Article Heuristic evaluation of virtual reality applications Get access Alistair Sutcliffe, Alistair Sutcliffe ∗ Centre for HCI Design, School of Informatics, University of Manchester, P.O. Box 88, Manchester M60 1QD, UK ∗ Corresponding author. Tel.: +44-0-161-200-3315; fax: +44-0+161-200-3324 Search for other works by this author on: Oxford Academic Google Scholar Brian Gault Brian Gault Centre for HCI Design, School of Informatics, University of Manchester, P.O. Box 88, Manchester M60 1QD, UK Search for other works by this author on: Oxford Academic Google Scholar Interacting with Computers, Volume 16, Issue 4, August 2004, Pages 831–849, https://doi.org/10.1016/j.intcom.2004.05.001 Published: 20 June 2004 Article history Received: 25 July 2003 Revision received: 30 April 2004 Accepted: 01 May 2004 Published: 20 June 2004
Geographic Database for IVHS Management (1991)

In this paper, the authors describe an Intelligent Vehicle Highway Systems (IVHS) information management system which obtains information from road sensors, city maps, and event schedules, and generates information to driver, traffic controllers and researchers. The authors then extend the relational database to model traffic information in a relational database by abstract data types and triggers.
IRF8 Interacts with the BCL6 Co-repressor, BCOR (109.5) (2012)

Abstract BCOR has been discovered as a BCL-6 interacting co-repressor, but little is known about its biological activities in normal B cell development and function. Previously, we found that the interferon regulatory factor 8 (IRF8), also known as interferon consensus sequence-binding protein, ICSBP, directly targets a large number of genes in germinal center (GC) B cells including BCL6. In this study, we screened potential binding partners of IRF8 using a retrovirus-based protein complementation assay in a mouse pre-B cell line. We found that IRF8 interacts directly with BCOR and the alpha helical region of IRF8 is required for this interaction. Using a siRNA-mediated IRF8 knockdown mouse B cell lymphoma cell line, we showed that IRF8 represses BCOR and enhances BCL6 transcription. Taken together, these data suggest that a complex comprised of BCOR-IRF8 modulates BCL6 associated transcriptional regulation of B cell function. Additional studies to evaluate the possible role of BCOR-IRF8 complex are in progress.
The Patch Transform (2009)

The patch transform represents an image as a bag of overlapping patches sampled on a regular grid. This representation allows users to manipulate images in the patch domain, which then seeds the inverse patch transform to synthesize modified images. Possible modifications include the spatial locations of patches, the size of the output image, or the pool of patches from which an image is reconstructed. When no modifications are made, the inverse patch transform reduces to solving a jigsaw puzzle. The inverse patch transform is posed as a patch assignment problem on a Markov random field (MRF), where each patch should be used only once and neighboring patches should fit to form a plausible image. We find an approximate solution to the MRF using loopy belief propagation, introducing an approximation that encourages the solution to use each patch only once. The image reconstruction algorithm scales well with the total number of patches through label pruning. In addition, structural misalignment artifacts are suppressed through a patch jittering scheme that spatially jitters the assigned patches. We demonstrate the patch transform and its effectiveness on natural images.
LayerMover: Storage Migration of Virtual Machine across Data Centers Based on Three-Layer Image Structure (2016)

Live Virtual Machine (VM) migration across data centers is an important support technology which will bring benefits to both cloud providers and users. At present, VM storage migration becomes the bottleneck of this technology as it explores only the static data feature of image files without much consideration on data semantics. In this paper, we propose a new space-efficient VM image structure-three-layer structure. According to different functions and features, the data of a VM are separated into an Operating System (OS) layer, a Working Environment (WE) layer, and a User Data (UD) layer. On basis of three-layer image structure, we design a novel VM storage migration system-LayerMover. It tries to improve the migration performance through optimizing current data deduplication methods. To evaluate the potential performance gains of LayerMover, we explore the similarity features of WE layer data. Our experimental results show that the similarity ratio between different images can reach 70%. Our experiments about the VM storage migration performance of LayerMover show that it can be 27% faster than current migration methods for twolayer image structures under our experimental environments. When migrating a VM in a second round or migrating a batch of VMs, LayerMover will see more performance improvement.
The early literature on Mallophaga. Part II, 1763-1775 (1951)

This is the second part of a series of papers in which it is intended to review the species of Mallophaga described between 1758 and 1818. This part deals with the twenty species described by G. A. Scopoli in 1763 and 1772, the single species described by Pontoppidan in 1763, and the three species described by J. C. Fabricius in 1775.
Desorption of single-stranded nucleic acids from graphene oxide by disruption of hydrogen bonding (2013)

Graphene oxide (GO) is known to interact with single-stranded nucleic acids through pi-stacking interactions and hydrogen bonds between the nucleobases and the hexagonal cells of GO. It also quenches the fluorescence when the fluorophore comes near to the GO mesh. When single-stranded (ss) regions of either DNA or RNA are present, those regions were adsorbed onto the surface of GO with a quenching of fluorescence located proximally to the GO surface. We demonstrated that bound single-stranded nucleic acids can be readily dissociated from GO by disrupting hydrogen bonding with urea, which was confirmed with fluorescence measurement and gel electrophoresis. Hydrogen bonding mainly contributes to the interaction between GO and single-stranded nucleic acids such as ssDNA and RNA. The GO-coated mesoporous silica nanoparticles (GO-MSNs) were synthesized for better separation of RNAs from cells. Cellular RNAs were readily adsorbed and eluted with ease by using GO-MSN and urea, respectively, demonstrating that GO-MSN and urea elution is a facile RNA extraction method.
Fluorescence lifetime excitation cytometry by kinetic dithering (2014)

Flow cytometers are powerful high-throughput devices that capture spectroscopic information from individual particles or cells. These instruments provide a means of multi-parametric analyses for various cellular biomarkers or labeled organelles and cellular proteins. However, the spectral overlap of fluorophores limits the number of fluorophores that can be used simultaneously during experimentation. Time-resolved parameters enable the quantification of fluorescence decay kinetics, thus circumventing common issues associated with intensity-based measurements. This contribution introduces fluorescence lifetime excitation cytometry by kinetic dithering (FLECKD) as a method to capture multiple fluorescence lifetimes using a hybrid time-domain approach. The FLECKD approach excites fluorophores by delivering short pulses of light to cells or particles by rapid dithering and facilitates measurement of complex fluorescence decay kinetics by flow cytometry. Our simulations demonstrated a resolvable fluorescence lifetime value as low as 1.8 ns (±0.3 ns) with less than 20% absolute error. Using the FLECKD instrument, we measured the shortest average fluorescence lifetime value of 2.4 ns and found the system measurement error to be ±0.3 ns (SEM), from hundreds of monodisperse and chemically stable fluorescent microspheres. Additionally, we demonstrate the ability to detect two distinct excited state lifetimes from fluorophores in single cells using FLECKD. This approach presents a new ability to resolve multiple fluorescence lifetimes while retaining the fluidic throughput of a cytometry system. The ability to discriminate more than one average fluorescence lifetime expands the current capabilities of high-throughput and intensity-based cytometry assays as the need to tag one single cell with multiple fluorophores is now widespread.
Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods (2003)

Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.
Tuning of successively scanned two monolithic Vernier-tuned lasers and selective data sampling in optical comb swept source optical coherence tomography (2013)

Monolithic Vernier tuned super-structure grating distributed Bragg reflector (SSG-DBR) lasers are expected to become one of the most promising sources for swept source optical coherence tomography (SS-OCT) with a long coherence length, reduced sensitivity roll-off, and potential capability for a very fast A-scan rate. However, previous implementations of the lasers suffer from four main problems: 1) frequencies deviate from the targeted values when scanned, 2) large amounts of noise appear associated with abrupt changes in injection currents, 3) optically aliased noise appears due to a long coherence length, and 4) the narrow wavelength coverage of a single chip limits resolution. We have developed a method of dynamical frequency tuning, a method of selective data sampling to eliminate current switching noise, an interferometer to reduce aliased noise, and an excess-noise-free connection of two serially scanned lasers to enhance resolution to solve these problems. An optical frequency comb SS-OCT system was achieved with a sensitivity of 124 dB and a dynamic range of 55-72 dB that depended on the depth at an A-scan rate of 3.1 kHz with a resolution of 15 μm by discretely scanning two SSG-DBR lasers, i.e., L-band (1.560-1.599 μm) and UL-band (1.598-1.640 μm). A few OCT images with excellent image penetration depth were obtained.
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function (2004)

Interaction of p120 with juxtamembrane domain (JMD) of VE-cadherin has been implicated in regulation of endothelial cell-cell adhesion. We used a number of approaches to alter the level of p120 available for binding to VE-cadherin as a means to investigate the role of p120-VE-cadherin interaction in regulation of barrier function in confluent endothelial monolayers. Expression of an epitope-tagged fragment corresponding to JMD of VE-cadherin resulted in a decrease in endothelial barrier function as assessed by changes in albumin clearance and electrical resistance. Binding of JMD-Flag to p120 resulted in a decreased level of p120. In addition to decreasing p120 level, expression of JMD also decreased level of VE-cadherin. Expression of JMD also caused an increase in MLC phosphorylation and rearrangement of actin cytoskeleton, which, coupled with decreased cadherin, can contribute to loss of barrier function. Reducing p120 by siRNA resulted in a decrease in VE-cadherin, whereas increasing the level of p120 increased the level of VE-cadherin, demonstrating that p120 regulates the level of VE-cadherin. Overexpression of p120 was, however, associated with decreased barrier function and rearrangement of the actin cytoskeleton. Interestingly, expression of p120 was able to inhibit thrombin-induced increases in MLC phosphorylation, suggesting that p120 inhibits activation of Rho/Rho kinase pathway in endothelial cells. Excess p120 also prevented JMD-induced increases in MLC phosphorylation, correlating this phosphorylation with Rho/Rho kinase pathway. These findings show p120 plays a major role in regulating endothelial barrier function, as either a decrease or increase of p120 resulted in disruption of permeability across cell monolayers.
The music education program at Ainslie school (1999)

The Music Education Program (MEP) at Ainslie Primary School (APS) and Red Hill Primary School (RHPS) was initiaced by The Canberra School of Music (CSM) in 1984. It was conceived and developed by Anna de Borah and Susan West. It was the first time that a tertiary music institution had committed staff and funding to run a specialist music literacy program in government primary schools through its already existing funding allocations. Over the years, the issue of funding has been a constant problem and the source of discussion (and argument!) between federal and territorial funding bodies. Currently, the classroom program itself is funded through ACT Government grants for community involvement and the associated pedagogy programs are funded via the Federal grants for tertiary euducation.