gebhart/patrepeval huggingface dataset:
Enforced IL-10 Expression Confers Type 1 Regulatory T Cell (Tr1) Phenotype and Function to Human CD4+ T Cells (2012)

Type 1 regulatory T (Tr1) cells are an inducible subset of CD4(+) Tr cells characterized by high levels of interleukin (IL)-10 production and regulatory properties. Several protocols to generate human Tr1 cells have been developed in vitro. However, the resulting population includes a significant fraction of contaminating non-Tr1 cells, representing a major bottleneck for clinical application of Tr1 cell therapy. We generated an homogeneous IL-10-producing Tr1 cell population by transducing human CD4(+) T cells with a bidirectional lentiviral vector (LV) encoding for human IL-10 and the marker gene, green fluorescent protein (GFP), which are independently coexpressed. The resulting GFP(+) LV-IL-10-transduced human CD4(+) T (CD4(LV-IL-10)) cells expressed, upon T-cell receptor (TCR) activation, high levels of IL-10 and concomitant low levels of IL-4, and markers associated with IL-10. Moreover, CD4(LV-IL-10) T cells displayed typical Tr1 features: the anergic phenotype, the IL-10, and transforming growth factor (TGF)-β dependent suppression of allogeneic T-cell responses, and the ability to suppress in a cell-to-cell contact independent manner in vitro. CD4(LV-IL-10) T cells were able to control xeno graft-versus-host disease (GvHD), demonstrating their suppressive function in vivo. These results show that constitutive over-expression of IL-10 in human CD4(+) T cells leads to a stable cell population that recapitulates the phenotype and function of Tr1 cells.
Danièle Alexandre-Bidon et Monique Crosson, L'Enfant à l'ombre des cathédrales (1987)

Lett Didier. Danièle Alexandre-Bidon et Monique Crosson, L'Enfant à l'ombre des cathédrales. In: Médiévales, n°12, 1987. Toutes les routes mènent à Byzance, sous la direction de Evelyne Patlagean . pp. 129-130.
Development of test structures for characterization of the fabrication and performance of radiation-hardened charge-coupled device (CCD) imagers : (1980)

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Tomato leaf curl Yunnan virus-encoded C4 induces cell division through enhancing stability of Cyclin D 1.1 via impairing NbSKη -mediated phosphorylation in Nicotiana benthamiana (2018)

The whitefly-transmitted geminiviruses induce severe developmental abnormalities in plants. Geminivirus-encoded C4 protein functions as one of viral symptom determinants that could induce abnormal cell division. However, the molecular mechanism by which C4 contributes to cell division induction remains unclear. Here we report that tomato leaf curl Yunnan virus (TLCYnV) C4 interacts with a glycogen synthase kinase 3 (GSK3)/SHAGGY-like kinase, designed NbSKη, in Nicotiana benthamiana. Pro32, Asn34 and Thr35 of TLCYnV C4 are critical for its interaction with NbSKη and required for C4-induced typical symptoms. Interestingly, TLCYnV C4 directs NbSKη to the membrane and reduces the nuclear-accumulation of NbSKη. The relocalization of NbSKη impairs phosphorylation dependent degradation on its substrate-Cyclin D1.1 (NbCycD1;1), thereby increasing the accumulation level of NbCycD1;1 and inducing the cell division. Moreover, NbSKη-RNAi, 35S::NbCycD1;1 transgenic N. benthamiana plants have the similar phenotype as 35S::C4 transgenic N. benthamiana plants on callus-like tissue formation resulted from abnormal cell division induction. Thus, this study provides new insights into mechanism of how a viral protein hijacks NbSKη to induce abnormal cell division in plants.
Expression of the Opioid Growth Factor, [Met<sup>5</sup>]‐Enkephalin, and the Zeta Opioid Receptor in Head and Neck Squamous Cell Carcinoma (1997)

Despite the prevalence of cancers of the head and neck, survival rates have not changed in the past few decades. Recent work has implicated peptide growth factors and their receptors in the genesis and progression of head and neck squamous cell carcinoma. Opioid growth factor (OGF, [Met5]-enkephalin) is a tonically active, autocrine and/or paracrine produced, inhibitory factor that influences the growth of normal and abnormal cells and tissues. This peptide interacts with the zeta (zeta) opioid receptor to modulate cellular proliferation, migration, and survival. Both OGF and the zeta receptor are present in mammalian tongue epithelium and skin, and modulate DNA synthesis. In the present study we examined the presence and distribution of OGF and the zeta opioid receptor in the head and neck squamous cell carcinomas from seven individuals. All specimens expressed this growth factor and its receptor regardless of tumor stage, location, and histologic grade. Immunoreactivity for both OGF and the zeta receptor were associated with the cytoplasm but not the nucleus in cells of each of these carcinomas. Our findings that a potent negative growth regulator and its receptor are present in head and neck squamous cell carcinoma lead us to suggest that OGF may modulate the growth of these types of cancers.
The μsystem: Providing light‐weight concurrency on shared‐memory multiprocessor computers running UNIX (1990)

Abstract This paper presents a description of the μSystem, which is a library of C routines that provide light‐weight concurrency on uniprocessor and multiprocessor computers running the UNIX operating system. A discussion of the run‐time structure of a μSystem program is given, which includes the following concepts: coroutines, tasks, virtual processors and clusters. Next the routines that implement these concepts are discussed in detail. Finally, some performance figures from the μSystem are given and discussed, followed by a comparison of the μSystem with other similar systems.
Development of a gas chromatographic selected ion monitoring assay for thyroxine (T4) in human serum (1984)

The components of a gas chromatographic mass spectrometric-selected ion monitoring (SIM) assay for thyroxine (T4) in human serum are described. The internal standard for the assay was synthesized from deuterium-labelled 3,5-diiodotyrosine and 3,5-diiodo-4-hydroxyphenylpyruvic acid. A novel method was developed for isolating the products of the coupling reaction. The results obtained by gas chromatography mass spectrometry SIM were compared with those of radioimmunoassay. The gas chromatographic mass spectrometric SIM assay would form the basis of a reference assay for T4.
Degradation mechanisms of organic ferroelectric field-effect transistors used as nonvolatile memory (2009)

Organic ferroelectric field-effect transistors were fabricated by inkjet printing for use as nonvolatile memory. Changes in device hysteresis were measured for 7 days to determine the limiting properties that restrict memory retention time. It was found that shifts in threshold voltage contributed to ∼55% of the reduction in transistor current, while decreased dielectric capacitance and reduced semiconductor mobility accounted for ∼30% and ∼15% of the current decay, respectively. The decrease in mobility and the shifts in threshold voltages are caused by remnant dipolar alignment in the ferroelectric insulator, and the reduction in gate capacitance is explained by injected charges in the ferroelectric dielectric. A method to calibrate and extract the input switching voltage is presented, and this calibration accounts for variations in device characteristics with time and allows the ferroelectric transistors to be used as analog memories.
Platinum-Based Drugs in Cancer Therapy (2000)

Part I: Platinum Chemistry. The Chemistry of Cisplatin in Aqueous Solution, Susan J. Berners-Price and Trevor G. Appleton. Chemistry and Structural Biology of 1,2-Interstrand Adducts of Cisplatin, Viktor Brabec. Transplatin-Modified Oligonucleotides as Potential Antitumor Drugs, Marc Leng, Annie Schwartz, and Marie-Josephe Giraud-Panis. Part II: Platinum Biochemistry. Cisplatin Accumulation, Paul A. Andrews. Cisplatin Resistance in Ovarian Cancer: Mismatch Repair and Engagement of Apoptosis, Robert Brown. DNA Adduct Tolerance and Bypass, Stephen G. Chaney and Alexandra Vaisman. How Does Cisplatin Kill Cells? Daniel Fink and Stephen B. Howell. Part III: Clinical Antitumor Activity and Toxicology of Platinum Drugs. Clinical Experience with Cisplatin and Carboplatin, M. S. Highley and A. H. Calvert. Clinical Experience: Platinum and Taxanes, Michelle Vaughan, Francisco Sapunar, and Martin Gore. Clinical Experience: DACH-Based Platinum Drugs, Peter J. O'Dwyer, James P. Stevenson, and Steven W. Johnson. Clinical Toxicology of Platinum-Based Cancer Chemotherapeutic Agents, Mark J. McKeage. Toxicology and Regulatory Aspects of Platinum Drugs, Diana L. Clark, Paul A. Andrews, D. D. Smith, Joseph J. DeGeorge, Robert L. Justice, and Julie G. Beitz. Part IV: New Platinum Drugs of the Future. New Platinum Drugs: The Pathway to Oral Therapy, Lloyd R. Kelland. Polynuclear Charged Platinum Compounds as a New Class of Anticancer Agents: Toward a New Paradigm, Nicholas Farrell. Index.
Enzymatic degradation of synthetic poly(.ALPHA.-amino acid) fibers. [2]. Copoly(N-hydroxyethyl-L-glutamine/.GAMMA.-methyl-L-glutamate) fiber. (1987)

Two component random copolypeptide (PHEG-co-PMLG) fibers consisting of N-hydroxyethyl-L-glutamine and γ-methyl-L-glutamate with different copolymer composition were prepared by a partial aminolysis reaction of poly (γ-methyl-L-glutamate) fibers with 2-amino-1-ethanol, followed by crosslinking reaction with 1, 8-octamethylenediamine. The tensile properties of these hydrophilic fibers were highly dependent on the degree of swelling Qw in PBS solution (pH=7.4), and showed a typical elastomeric behavior in wet-state. Biodegradation behaviors of PHEG-co-PMLG fibers were performed in vitro, including enzymatic degradation and mechanical properties to simulate in vivo fiber degradation. The rate of degradation of these hydrophilic fibers in vitro by pronase E was also highly dependent on Qw of fibers. It was pointed out that the enzymatic degradation of these fibers was processed on the surface area of the fibers, and that the rate of degradation of these fibers was analyzed by the first order reaction rate concerning the effective surface area of the fiber. This fact was also demonstrated from the direct observation by scanning electron microscopy. The behavior of the decrease in the tensile strength as a function of the decrease in the fiber weight was characteristic for the enzymatic degradable fibers and quite different from that for the non-enzymatic degradable fibers such as polyglycolic acid fiber. The tissue reaction was observed to be extremely slight for these hydrophilic fibers, which have been replaced by the connective tissue quite safely during the absorption process.
The Chemical Structure of Mumbaistatin, a Novel Glucose-6-phosphate Translocase Inhibitor Produced by Streptomyces sp. DSM 11641. (2001)

The characterization of the structure of mumbaistatin (1), an effective inhibitor of the glucose-6-phosphatase system (EC 3.1.3.9), is reported. Isolation of mumbaistatin from cultures of Streptomyces sp. DSM 11641 was achieved by anion-exchange and reversed-phase chromatography. The acid-labile inhibitor was methylated for the structure determination. Single-crystal X-ray structure analysis of a triply methylated dehydration product, C31H24O11, revealed the structure of an aromatic dispirodiketal (2), a compound containing a previously undescribed ring system. Extensive 2D-NMR experiments with mumbaistatin and with the methylation products showed that mumbaistatin itself possesses the hydroxydiketodicarboxylic acid structure 1, C28H20O12, which, in the presence of acid or upon activation through methyl ester formation, undergoes self-condensation with loss of water to the dispirodiketal form (2). Mumbaistatin is an anthraquinone derivative, whose open-chain diketo form acts as a specific and powerful inhibitor of glucose-6-phosphate translocase: IC50=5 nM. The activity towards the same enzyme of the cyclized dispirodiketal derivatives is roughly one thousand times lower.
Restriction endonuclease analysis of Penicillium marneffei (1996)

Forty-six isolates of Penicillium marneffei were differentiated into two DNA types on the basis of their restriction fragment length polymorphisms. Of the 22 human isolates of P. marneffei, 16 (72.7%) were type I and 6 (27.3%) were type II. Of the 23 bamboo rat isolates, 20 from Rhizomys sumatrensis were type I and 3 from Cannomys badius were type II. The soil isolate was type II. These data represent the first molecular epidemiological study of this important emerging fungal pathogen.
Highly Enantioselective Asymmetric Isomerization of Primary Allylic Alcohols with an Iridium–N,P Complex (2011)

Access to chiral aldehydes: The asymmetric isomerization of primary allylic alcohols was studied with a bicyclic phosphine-oxazoline iridium catalyst. This method displays a broad substrate scope and leads to the desired chiral aldehydes with excellent enantioselectivities (see scheme; R1, R2=Ar or alkyl). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Dense Breast Tissues May Hold Increased Cancer Risk for Some (1994)

Journal Article Dense Breast Tissues May Hold Increased Cancer Risk for Some Get access Susan Jenks Susan Jenks Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 86, Issue 8, 20 April 1994, Pages 578–580, https://doi.org/10.1093/jnci/86.8.578 Published: 20 April 1994
A Benchmark Dataset and Saliency-Guided Stacked Autoencoders for Video-Based Salient Object Detection (2017)

Image-based salient object detection (SOD) has been extensively studied in past decades. However, video-based SOD is much less explored due to the lack of large-scale video datasets within which salient objects are unambiguously defined and annotated. Toward this end, this paper proposes a video-based SOD dataset that consists of 200 videos. In constructing the dataset, we manually annotate all objects and regions over 7650 uniformly sampled keyframes and collect the eye-tracking data of 23 subjects who free-view all videos. From the user data, we find that salient objects in a video can be defined as objects that consistently pop-out throughout the video, and objects with such attributes can be unambiguously annotated by combining manually annotated object/region masks with eye-tracking data of multiple subjects. To the best of our knowledge, it is currently the largest dataset for video-based salient object detection. Based on this dataset, this paper proposes an unsupervised baseline approach for video-based SOD by using saliency-guided stacked autoencoders. In the proposed approach, multiple spatiotemporal saliency cues are first extracted at the pixel, superpixel, and object levels. With these saliency cues, stacked autoencoders are constructed in an unsupervised manner that automatically infers a saliency score for each pixel by progressively encoding the high-dimensional saliency cues gathered from the pixel and its spatiotemporal neighbors. In experiments, the proposed unsupervised approach is compared with 31 state-of-the-art models on the proposed dataset and outperforms 30 of them, including 19 image-based classic (unsupervised or non-deep learning) models, six image-based deep learning models, and five video-based unsupervised models. Moreover, benchmarking results show that the proposed dataset is very challenging and has the potential to boost the development of video-based SOD.
The <i>rad18</i> Gene of <i>Schizosaccharomyces pombe</i> Defines a New Subgroup of the SMC Superfamily Involved in DNA Repair (1995)

The rad18 mutant of Schizosaccharomyces pombe is very sensitive to killing by both UV and gamma radiation. We have cloned and sequenced the rad18 gene and isolated and sequenced its homolog from Saccharomyces cerevisiae, designated RHC18. The predicted Rad18 protein has all the structural properties characteristic of the SMC family of proteins, suggesting a motor function--the first implicated in DNA repair. Gene deletion shows that both rad18 and RHC18 are essential for proliferation. Genetic and biochemical analyses suggest that the product of the rad18 gene acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair. This second repair pathway involves the products of the rhp51 gene (the homolog of the RAD51 gene of S. cerevisiae) and the rad2 gene.
Evaluation of Serum Levels of Interleukin (IL)–6, IL‐10, and Tumor Necrosis Factor–α in Patients with Crimean‐Congo Hemorrhagic Fever (2006)

We investigated the role played by cytokines in the mortality of patients with Crimean-Congo hemorrhagic fever (CCHF). Serum levels of several cytokines were measured in 3 patients with fatal CCHF and in 27 patients with nonfatal CCHF. Levels of interleukin (IL)-6 (P< or = .001) and tumor necrosis factor (TNF)-alpha (P = .004) were significantly higher in patients with fatal CCHF than in patients with nonfatal CCHF, whereas levels of IL-10 were not significantly different between the 2 groups (P = .937). Disseminated intravascular coagulation (DIC) scores were also higher in the patients with fatal CCHF (P = .023). Levels of IL-6 and TNF-alpha were positively correlated with DIC scores, whereas levels of IL-10 were negatively correlated with DIC scores. In conclusion, these findings demonstrate that proinflammatory cytokines play a major role in the mortality of patients with CCHF.
Organization of Nucleosides Supported by Boronic-Acid-Appended Poly(<scp>l</scp>-lysine): Creation of a Novel RNA Mimic (2001)

Abstract Boronic-acid-appended poly(l-lysine) (1) can form anionic boronate esters with diol derivatives in aqueous solution. One can thus expect that when nucleosides are mixed with 1, the resultant complexes will satisfy the basic structural characteristics of RNA, viz., one-dimensionally organized nucleic bases and anionic charges. To obtain insights into this novel RNA mimic, the solution properties of adenosine (Ad) were estimated in the absence and the presence of 1. The large absorption and CD spectral changes were observed at pH 6–10 where the poly(l-lysine) main chain tends to adopt an α-helix-rich conformation. On the basis of the fact that the magnitude of the spectral changes is much larger than the fraction of complexed Ad molecules, the polymer-supported organization mode was proposed: that is, Ad molecules complexed by covalent bonds with 1 act as clusters to induce further organization of uncomplexed Ad molecules by noncovalent interactions. The proposal was further confirmed by 1H NMR spectroscopy and dynamic light scattering. On the other hand, none of these spectral changes were observed for 2′-deoxyadenosine. The 1–Ad complex thus formed can interact with complementary poly(U) but not with noncomplementary poly(C), indicating that it can act as a novel RNA mimic.
Safety, immunogenicity, and lot stability of the whole cell/recombinant B subunit (WC/rCTB) cholera vaccine in Peruvian adults and children. (1999)

To assess the safety, immunogenicity, and lot stability of the whole cell/recombinant B subunit cholera vaccine, 2 lots manufactured in June 1991 and February 1992 were tested in January 1995. Two oral doses of vaccine or placebo given 2 weeks apart were given with buffer to 216 Peruvian adults and children. Symptoms were elicited for 3 days after each dose. Serum and plasma specimens obtained from each volunteer before vaccination and 10-14 days after the second dose were tested for vibriocidal and anti-cholera toxin antibodies. The vaccine was well-tolerated. Nearly half of the 100 vaccinees had pre-vaccination vibriocidal titers > or = 1:40. Elevated titers were observed in 22% of 37 children 2-5 years of age compared with 66% of 63 vaccinees 6-65 years (P < 0.001). A > or =2-fold serum vibriocidal response was observed in 55% of 100 vaccinees and 6% of 32 placebo recipients. An elevated pre-vaccination titer (< or =1:40) did not change the proportion of vaccinees who responded with a > or =2-fold increase in vibriocidal titer (51% versus 59%, difference not significant), but did change the proportion responding with a > or =4-fold increase (41% versus 22%; P < 0.05). The vibriocidal seroconversion rate was lowest in children 2-5 years old despite low pre-vaccination titers. Two-fold or greater serum antitoxic responses in IgA and IgG were observed in >90% of the vaccinees; > or =4-fold responses were seen in 65-70% of the vaccinees with a 6-8-fold increase over baseline. Plasma specimens were as good as sera for determining anti-toxic antibodies by ELISA, but were less satisfactory for determining vibriocidal antibody titers.
Expression of the vitamin D metabolizing enzyme CYP24A1 at the annulus of human spermatozoa may serve as a novel marker of semen quality (2012)

Vitamin D (VD) is important for male reproduction in mammals and the VD receptor (VDR) and VD-metabolizing enzymes are expressed in human spermatozoa. The VD-inactivating enzyme CYP24A1 titrates the cellular responsiveness to VD, is transcriptionally regulated by VD, and has a distinct expression at the sperm annulus. Here, we investigated if CYP24A1 expression serves as a marker for VD metabolism in spermatozoa, and whether CYP24A1 expression was associated with semen quality. We included 130 men (53 healthy young volunteers and 77 subfertile men) for semen analysis and immunocytochemical (ICC) detection of CYP24A1. Another 40 men (22 young, 18 subfertile) were tested for in vitro effects of 1,25(OH)(2)D(3) on intracellular calcium concentration ([Ca(2+)](i)) and sperm motility. Double ICC staining showed that CYP24A1 and VDR were either concomitantly expressed or absent in 80% of the spermatozoa from young men. The median number of CYP24A1-expressing spermatozoa was 1% in subfertile men and thus significantly (p < 0.0005) lower than 25% in spermatozoa from young men. Moreover, CYP24A1 expression correlated positively with total sperm count, -concentration, -motility and -morphology (all p < 0.004), and the percentage of CYP24A1-positive spermatozoa increased (15 vs. 41%, p < 0.0005) after percoll-gradient-centrifugation. We noticed that the presence of >3% CYP24A1-positive spermatozoa distinguished young men from subfertile men with a sensitivity of 66.0%, a specificity of 77.9% and a positive predictive value of 98.3%. Functional studies revealed that 1,25(OH)(2)D(3) increased [Ca(2+)](i) and sperm motility in young healthy men, while 1,25(OH)(2)D(3) was unable to increase motility in subfertile patients. In conclusion, we suggest that CYP24A1 expression at the annulus may serve as a novel marker of semen quality and an objective proxy for sperm function.
HMM analysis of general state-space models (2011)

These notes explain how to use a hidden Markov model (HMM) approach for analysing possibly highly nonlinear time series data in a state-space formulation. The text introduces the general state-space model and gives an overview of other methods for ltering and smoothing ranging from the simple linear and Gaussian case to the fully general case. A discretization of the state-space is instrumental to the HMM approach and the choice of discretization is therefore discussed. The lter and smoothing recursions for the hidden Markov model are presented and applied to the standard benchmark model known from the literature on nonlinear time series. Finally, as an example, the parameters of a stochastic volatility model are estimated with maximum likelihood and the results are compared with an Monte Carlo based estimation procedure.