gebhart/patrepeval huggingface dataset:
FcϵRI-mediated Induction of Nuclear Factor of Activated T-cells (1995)

Nuclear factor of activated T-cells (NFAT) is a transcriptional activator that binds to the interleukin-2 promoter and is believed to be responsible for T-cell-specific interleukin-2 gene expression. Here we demonstrate using electrophoretic mobility shift assays that nuclear NFAT can be induced in the rat basophilic leukemia (RBL-2H3) mast cell line and rat bone marrow-derived mast cells upon cross-linkage of the high affinity receptor (Fc epsilon RI) for immunoglobulin E (IgE). Receptor-dependent activation of NFAT was mimicked by the combination of the protein kinase C activator phorbol myristate acetate and the calcium ionophore ionomycin. The induced binding activity was specific for the NFAT recognition motif because competition with nonradioactive NFAT oligonucleotide abolished the DNA binding activity, whereas nonradioactive oligonucleotides recognized by the transcription factors NF kappa B, glucocorticoid receptors, and TFIID did not. An oligonucleotide representing the AP-1 recognition sequence also blocked the NFAT DNA binding activity, as did a combination of anti-Fos and anti-Jun antibodies. Using electrophoretic mobility shift assays, AP-1-binding proteins were found to be induced in RBL-2H3 cells under the same conditions as was the NFAT binding activity. Together these data suggest that the NFAT complex in mast cells contains Fos and Jun proteins as does NFAT in T-cells. The appearance of nuclear NFAT binding activity was dependent in part upon calcium mobilization, as buffering the antigen-induced calcium rise with intracellular BAPTA strongly inhibited NFAT activation. Prevention of calcium influx with external EGTA also inhibited NFAT activation, indicating that release of calcium from internal stores was insufficient for sustained activation of mast cell NFAT. Cyclosporin A, a potent inhibitor of the calmodulin-dependent phosphatase calcineurin, blocked the induction of NFAT-DNA binding activity, implicating calcineurin as a key signaling enzyme in this pathway. These results suggest that NFAT is present in the mast cell line RBL-2H3 and in primary bone marrow-derived mast cells, is similar in subunit composition to the T-cell NFAT, and may play a role in calcium-dependent signal transduction in mast cells.
The Relation between Age and Mean Length of Utterance in Morphemes (1981)

The relationship between child age and mean length of utterance measured in morphemes (MLU) was studied in a sample of 123 middle- to upper-middle-class midwestern children, aged 17 to 59 months, conversing with mothers in free play. A significant correlation was found between age and MLU: r = .88. Age accounted for 78% of the variance when MLU was regressed on age; and MLU accounted for 77% of the variance when age was regressed on MLU. Significant nonlinear components accounted only for an additional 1% and 3% (respectively) of the variance. Ranges within one standard deviation (SD) were estimated for predicted MLUs and derived for predicted ages on the basis of linear regression. MLU increased at an average rate of 1.2 morphemes per year; predicted variability in predicted MLU increased with age. These findings can play a useful role in three activities when the children studied are from populations similar to the one sampled here, and MLUs are obtained in the same way. They can aid in (1) finding children whose development of productive syntax requires further diagnostic evaluation; (2) finding children at a particular linguistic stage quickly; and (3) predicting the age most likely to be associated with a given MLU. The substitution of local MLU norms, sampled from the population and ages of clinical interest, can further sharpen these evaluations.
Advances in photochemistry, Vol. 14 (1988)

This volume reflects the significant advances in experimentation and interpretation in the field, presenting new insights into the history of molecules that absorb radiation. It is part of a series intended to explore the frontiers of photochemistry.
Nisin: a possible alternative or adjunct to nitrite in the preservation of meats (1981)

Nisin at 75 ppm (75 microgram/g) was superior to 150 ppm of nitrite in inhibiting outgrowth of Clostridium sporogenes PA3679 spores in meat slurries, which had been heated to simulate the process used for cooked ham. The inhibitory activity of nisin decreased as the spore load or pH of the slurries increased. Unlike nitrite, inhibition by nisin was unaffected by high levels of iron either as a constituent of meats or when added as an iron salt. In slurries treated with 75 ppm of nisin, refrigerated storage for 56 days resulted in depletion of nisin to a level low enough to allow outgrowth within 3 to 10 days if the slurries were subsequently abused at 35 degrees C. In contrast, a combination of 40 ppm of nitrite and either 75 or 100 ppm of nisin almost completely inhibited outgrowth in these slurries. The nisin-nitrite combination appeared to have a synergistic effect, and the low concentration of nitrite was sufficient to preserve the color in meats similar to that of products cured with 150 ppm of nitrite.
Evaluation of a lifetime‐based optode to measure oxygen in aquatic systems (2006)

In this article, we evaluate the performance of a commercially available lifetime‐based optode and compare it with data obtained by other methods. We performed a set of 10 different tests, including targeted laboratory evaluations and field studies, covering a wide range of situations from shallow coastal waters and wastewater treatment plants to abyssal depths. Our principal conclusion is that, owing to high accuracy (± 2 µM), long‐term stability (more than 20 months), lack of pressure hysteresis, and limited cross‐sensitivity, this method is overall more suitable for oxygen monitoring than other methods.
Identification of immunodominant autoantigens in rat autoimmune orchitis (2005)

Infection and inflammation of the genital tract are amongst the leading causes of male infertility. Experimental autoimmune orchitis (EAO) in the rat serves as a model for the investigation of inflammatory testicular impairment. In this study, experiments were conducted to identify the molecules that are responsible for eliciting the autoimmune attack on the testis. EAO was induced in in-bred Wistar rats by active immunization with testis homogenates (EAO group I). Development of disease was observed using histological techniques and a new non-invasive three-dimensional (3D) imaging technology for in vivo monitoring, termed flat-panel volumetric computed tomography (fpvCT). Examination of control and EAO testes demonstrated the superior image quality of high-resolution fpvCT. A proteomics approach using 2D SDS-PAGE and immunoblotting analysis with EAO sera identified 12 spots. Seven were subsequently identified by mass spectrometry as heat shock proteins 60 (Hsp60) and 70 (Hsp70), disulphide isomerase ER-60, alpha-1-anti-trypsin, heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1), sperm outer dense fibre major protein 2 (ODF-2), and phosphoglycerate kinase 1. Hsp70, ODF-2, hnRNP H1, and ER-60 were identified by all EAO sera studied. To test the capacity of the identified proteins to elicit testicular autoimmune disease, recombinant proteins were used either individually or in combination to immunize rats (EAO group II). In all groups, the incidence of EAO was 25%. Inflammatory-type (ED1+) and resident (ED2+) macrophages, lymphocytes (CD45RA+), and dendritic cells (Ox-62+) were strongly increased in EAO group II animals, comparable to the testes of EAO I rats. Pre-immunization with a low dose of recombinant Hsp 70, hnRNP H1 or ODF-2 before induction of EAO with testis homogenate significantly delayed the onset of EAO but could not prevent disease. The identification of testicular autoantigens will allow a better understanding of disease pathogenesis and could provide a basis for the development of novel therapies for inflammation-based male infertility.
Single-Walled Carbon Nanotubes Based Chemicapacitive Sensors (2012)

Carboxylated single-walled carbon nanotubes (SWNTs) based chemicapacitive gas sensors were fabricated by AC dielectrophoretically aligning SWNTs across microfabricated gold electrodes with controlled density/device resistance. Two different sensing configurations (i.e., horizontal/in-plane and vertical/out-of-plane) were utilized to compare their sensing performance. Upon exposure to water vapor at room temperature, the response (R = [(C--C0)/C0] x 100%) increased with an increase in water vapor concentration similar to that of resistance response. In horizontal configuration, the response was increased with an increase in device resistance which might be attributed to preferentially alignment of semiconducting SWNTs during initial phase of alignment. However, the response was independent of device resistance in vertical/out-of-plane configuration which indicated that the sensing mechanism is based on the change of dielectric constant of gate and atmosphere.
Improved stochastic modeling of shapes for content-based image retrieval (2003)

Recent advances in the stochastic modeling of shapes for content based image database retrieval are presented. These advances include an integrated approach to shape and color based retrieval, where the cues: color and shape, are both utilized in a local rather than a global way, as well as a novel deformation-tolerant method based on (pseudo-) two dimensional stochastic models. The stochastic modeling itself is based on the use of HMMs, whereas the feature extraction is a polar sampling technique which is also known as shape matrix. Previously, it was demonstrated that this combination of feature extraction and HMMs is able to perform an elastic matching, which is especially needed in sketch based image retrieval (S. Muller et al., 1998). The use of streams (sets of features that are assumed to be statistically independent) within the HMM framework allows the integration of shape and color derived features into a single model, thereby allowing one to control the influence of the different streams via stream weights. Furthermore, these stream weights can also be utilized in order to integrate weighting factors, which have been derived in the context of shape matrices, in order to achieve a more objective comparison between shapes. The weighting factors are based on the fact that the sampling density is not constant with the polar sampling raster.
Extraction of Steroid Conjugates from Urine Using Charcoal as an Adsorbent (1965)

The extraction of steroid conjugates from human urine using charcoal as an adsorbent has been investigated using urine specimens containing radioactive, naturally occurring conjugates of various steroid hormones. Following adsorption, the conjugates were removed from the charcoal by elution with ammoniacal ethanol. The recovered radioactive materials were examined by partition column chromatography in order to establish that the extracted metabolites remained in the conjugated form. The recoveries were comparable to those obtained by the ether-ethanol extraction procedure of Edwards et al. (1). Analysis of the conjugated metabolites of cortisol isolated by charcoal suggested that they were unaltered by the adsorption procedure. The results of this study disclose that adsorption on charcoal can serve as the basis for a simple and efficient method for the recovery of steroid conjugates from urine.
Tinnitus: a management model (1986)

A comprehensive model of tinnitus management is proposed. As it is rarely possible to abolish the symptom, management of the tinnitus patient must aim at precipitating the habituation process. The model is split into 'evaluation' and 'remediation' sections. In each section the various aspects of management are discussed. Together with traditional factors, the importance of psychological processes is stressed. The role of the expectations of the patient in limiting remedial possibilities is also discussed.
Citrus Yield Mapping System in Natural Outdoor Scenes using the Watershed Transform (2006)

A machine vision system consisting of two CCD color cameras, a DGPS receiver, anencoder and ultrasonic sensors was developed to obtain citrus yield information of a citrus grove. Atotal of 512 images from 32 trees were taken during static operation from a citrus grove located in theWinter Garden, Florida.<br><br>A fruit counting algorithm was developed in this research to estimate citrus yield. Noise removal andillumination adjustment were conducted. The color segmentation and marker-controlled Watershedalgorithm were developed to segment and split touching fruits. The algorithm was tested on 176images and the number of fruits counted by the algorithm was compared with the number of fruitscounted by human observation. The coefficient of determination, R2, was 0.83. Moreover, thenumber of fruits counted by the algorithm and actual harvested number of boxes was compared. Thecoefficient of determination was 0.64. An average fruit size was also determined from each imageusing ultrasonic measurements.
GLUT-4 NH2 terminus contains a phenylalanine-based targeting motif that regulates intracellular sequestration. (1993)

Expression of chimeras, composed of portions of two different glucose transporter isoforms (GLUT-1 and GLUT-4), in CHO cells had indicated that the cytoplasmic NH2 terminus of GLUT-4 contains important targeting information that mediates intracellular sequestration of this isoform (Piper, R. C., C. Tai, J. W. Slot, C. S. Hahn, C. M. Rice, H. Huang, D. E. James. 1992. J. Cell Biol. 117:729-743). In the present studies, the amino acid constituents of the GLUT-4 NH2-terminal targeting domain have been identified. GLUT-4 constructs containing NH2-terminal deletions or alanine substitutions within the NH2 terminus were expressed in CHO cells using a Sindbis virus expression system. Deletion of eight amino acids from the GLUT-4 NH2 terminus or substituting alanine for phenylalanine at position 5 in GLUT-4 resulted in a marked accumulation of the transporter at the plasma membrane. Mutations at other amino acids surrounding Phe5 also caused increased cell surface expression of GLUT-4 but not to the same extent as the Phe5 mutation. GLUT-4 was also localized to clathrin lattices and this colocalization was abolished when either the first 13 amino acids were deleted or when Phe5 was changed to alanine. To ascertain whether the targeting information within the GLUT-4 NH2-terminal targeting domain could function independently of the glucose transporter structure this domain was inserted into the cytoplasmic tail of the H1 subunit of the asialoglycoprotein receptor. H1 with the GLUT-4 NH2 terminus was predominantly localized to an intracellular compartment similar to GLUT-4 and was sequestered more from the cell surface than was the wild-type H1 protein. It is concluded that the NH2 terminus of GLUT-4 contains a phenylalanine-based targeting motif that mediates intracellular sequestration at least in part by facilitating interaction of the transporter with endocytic machinery located at the cell surface.
A Method to Determine Lysine Acetylation Stoichiometries (2014)

Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes. A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proportion of lysine residues that are acetylated. Here we describe a mass spectrometry method using a combination of isotope labeling and detection of a diagnostic fragment ion to determine the stoichiometry of protein lysine acetylation. Using this technique, we determined the modification occupancy for ~750 acetylated peptides from mammalian cell lysates. Furthermore, the acetylation on N-terminal tail of histone H4 was cross-validated by treating cells with sodium butyrate, a potent deacetylase inhibitor, and comparing changes in stoichiometry levels measured by our method with immunoblotting measurements. Of note we observe that acetylation stoichiometry is high in nuclear proteins, but very low in mitochondrial and cytosolic proteins. In summary, our method opens new opportunities to study in detail the relationship of lysine acetylation levels of proteins with their biological functions.
Size dependent oxygen buffering capacity of ceria nanocrystals (2010)

The structural, chemical and electronic changes of ceria as a function of decreasing particle size have been studied: at sizes below 5 nm the total amount of reducible oxygen dramatically increases due to superoxide formation on the ceria surface, a result indicative of a size dependent oxygen buffering capacity.
Bradykinin-induced amyloid precursor protein secretion: a protein kinase C-independent mechanism that is not altered in fibroblasts from patients with sporadic Alzheimer's disease (1998)

We treated human skin fibroblasts with bradykinin (BK) and observed a concentration-dependent increase in the release of soluble amyloid precursor protein (sAPP). The estimated EC50 for the observed effect is 2.8 nM, which is of the same order of magnitude as the reported Kd of BK binding in human skin fibroblasts. The effect of BK on sAPP secretion appears to be dependent on interaction of the ligand with the B2 type of BK receptors but independent of activation of protein kinase C. We also show that sAPP release after BK treatment in fibroblasts from patients with sporadic Alzheimer's disease is not different from control cells and is paralleled by equivalent levels of inositol trisphosphate production. A discussion of the differences from previously published work focuses on the possible divergent alterations in transduction systems in fibroblasts from patients with familial and sporadic Alzheimer's disease. Our results are the first example of receptor-mediated sAPP release in human skin fibroblasts and the first demonstration of the co-existence of protein kinase C-dependent and -independent mechanisms in these cells.
Effect of galvanizing conditions on the impact properties of high strength steels. (1990)

Charpy impact tests were carried out in accordance with an experimental design on high-strength low alloy rolled structural steel with a grade of 785MPa, and on high-strength bolt steels with a grade of between 785 and 1177MPa such as low carbon boron steel. The following results were obtained.(1) For the structural steel, impact values were not affected by the galvanizing conditions, but the impact values of the galvanized steel, under the restraint of plastic deformation by the cδ1 layer, which was harder than the steel, became lower by about 10 to 12% at the upper transition temperature curves.(2) For bolt steels, when the hardness of the steel was less than the cδ1 layer, galvanizing decreased the impact values of the steels at room. For galvanized SNCM steel, these value decreased by about 12% after shot blasting and 20% after acid pickling. The decrease for galvanized LCB steel was about 9%. When, on the other hand, the steel was harder than the cδ1 layer, these effects were not observed.The above results indicate that when the cδ1 layer is harder than the steel, impact values decrease under the restraint of plastic deformation by the cδ1 layer.
Spectral dispersion curves of polymeric birefringent textile fibers (2002)

Optical Fourier transform (OFT) polarizing microscopy combined with variable-wavelength interferometry (VAWI) was used for the determination of the spectral dispersion curves of birefringent textile fibers. We observed that the maximally bright circular fringe from the OFT technique corresponded to the anticoincidence case of the fiber fringe with the empty fringe from the VAWI technique and that the maximally dark fringe from the OFT technique corresponded to the coincidence case. With this combination of the two techniques, we identified the positions of the anticoincidence and coincidence when the VAWI technique was applied to overcome the difficulties of manually determining these positions. The intensity of the OFT patterns at the center was grabbed with a charge couple device camera and analyzed with a photodiode electrical circuit. The new observation was used for the determination of the spectral dispersion curves of the birefringence of polyethylene and aramide fibers. Microinterferograms are provided as illustrations. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 84: 2481–2488, 2002
Profiling of Serum Bile Acids in a Healthy Chinese Population Using UPLC–MS/MS (2015)

Bile acids (BAs) are a group of important physiological agents for cholesterol metabolism, intestinal nutrient absorption, and biliary secretion of lipids, toxic metabolites, and xenobiotics. Extensive research in the last two decades has unveiled new functions of BAs as signaling molecules and metabolic regulators that modulate hepatic lipid, glucose, and energy homeostasis through the activation of nuclear receptors and G-protein-coupled receptor signaling in gut-liver metabolic axis involving host-gut microbial co-metabolism. Therefore, investigation of serum BA profiles, in healthy human male and female subjects with a wide range of age and body mass index (BMI), will provide important baseline information on the BA physiology as well as metabolic homeostasis among human subjects that are regulated by two sets of genome, host genome, and symbiotic microbiome. Previous reports on age- or gender-related changes on BA profiles in animals and human showed inconsistent results, and the information acquired from various studies was highly fragmentary. Here we profiled the serum BAs in a large population of healthy participants (n = 502) and examined the impact of age, gender, and BMI on serum BA concentrations and compositions using a targeted metabonomics approach with ultraperformance liquid chromatography triple-quadrupole mass spectrometry. We found that the BA profiles were dependent on gender, age, and BMI among study subjects. The total BAs were significantly higher in males than in females (p < 0.05) and higher in obese females than in lean females (p < 0.05). The difference in BA profiles between male and female subjects was decreased at age of 50-70 years, while the difference in BA profiles between lean and obese increased for subjects aged 50-70 years. The study provides a comprehensive understanding of the BA profiles in healthy subjects and highlights the need to take into account age, gender, and BMI differences when investigating pathophysiological changes of BAs resulting from gastrointestinal diseases.