gebhart/patrepeval huggingface dataset:
Patterns of Hyaluronan Staining Are Modified by Fixation Techniques (1997)

The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The HA, particularly in the dermis, was best preserved in sections fixed in 10% acid-formalin with 70% ethanol. In contrast, sections fixed in the routine 10% neutral-buffered formalin had a much weaker intensity of HA staining. Furthermore, acid-formalin/ethanol-fixed sections retained much of their apparent HA after incubation with saline, in contrast to the neutral formalin-fixed sections, in which most of the stainable HA was lost. Such marked differences in staining intensity were not observed in slides stained with Alcian blue, a procedure pressumed to stain HA as well as other glycosaminoglycans. Staining using the HA binding peptide was entirely absent when sections were first preincubated in hyaluronidase, whereas similar Alcian blue-stained sections retained most of their staining intensity. Caution should be exercised in evaluating the distribution of HA in tissues using the HA binding peptide, particularly when different fixation techniques among several laboratories are being compared. In addition, the ability to evaluate the HA content of tissues using Alcian blue staining should be reconsidered. The sulfated glycosaminolglycans of the “ground substance” appear to be the predominant substrates for Alcian blue. (J Histochem Cytochem 45:1157–1163, 1997)
Artificial molecule realization of a magnetic wall (1997)

The design of magnetic materials can be achieved with artificial magnetic molecules formed by electrically small loop antennas loaded with passive electrical circuit elements. A time derivative Lorentz material response is achieved with a series resistor and capacitor load. It is shown that the parameters of this magnetic molecule can be selected to yield a time derivative Lorentz material that acts as a highly conducting magnetic wall.
Vaginitis: Case Reports and Brief Review (2002)

Vaginitis is the most frequently gynecologic diagnosis encountered by physicians who provide primary care to women. Accurate diagnosis can be elusive, complicating treatment. Most experts believe that up to 90% of vaginitis cases are secondary to bacterial vaginosis, vulvo-vaginal candidiasis, and trichomoniasis. The diagnosis of vaginitis is based on the patient's symptoms, the physical examination, the findings of microscopic examination of the wetmount and potassium hydroxide (KOH) preparations, and the results of the pH litmus test. Additional cultures and testing may be needed for difficult-to-diagnose cases.
Blastocyst attachment in the ovariectomized rat treated with an intrauterine injection of luteinizing hormone-releasing hormone (LRH) (1983)

Pregnant rats were bilaterally ovariectomized on day 3 and the blastocysts were maintained in a state of delayed implantation by the daily sc injection of 4 mg progesterone from day 3 to 6. On the morning of day 6 the rats received an intrauterine injection of 1 mg LRH in the right horn. A second group of rats was treated as above and was additionally injected with 2 mg of indomethacin on day 5 and 1 mg on the morning of day 6. Fifteen min prior to autopsy the animals were injected iv with a 0.5% solution of Evans Blue. The presence of uterine blue bands and the number of blastocysts recovered from the right and left (control) horns were recorded at 0.25, 0.5, 1.2 and 4 h after the injection of LRH. It was determined that the number of blastocysts flushed from the treated horn was significantly reduced below that of the control left horn within 15 min and at every autopsy interval thereafter. In addition, blue bands appeared in the treated horn as early as 15 min after the injection of LRH demonstrating that implantation had occurred in a few instances. No blue bands appeared in the control horns. The injection of indomethacin did not inhibit the attachment or implantation of blastocysts or the presence of blue bands in the treated horn. A possible role for blastocyst oestrogen is discussed.
Cylindrical Microdomain Orientation of PS-<i>b</i>-PMMA on the Balanced Interfacial Interactions: Composition Effect of Block Copolymers (2009)

ADVERTISEMENT RETURN TO ISSUEPREVNoteNEXTCylindrical Microdomain Orientation of PS-b-PMMA on the Balanced Interfacial Interactions: Composition Effect of Block CopolymersDu Yeol Ryu*, Sujin Ham, Eunhye Kim, Unyong Jeong, Craig J. Hawker§, and Thomas P. Russell⊥View Author Information† Department of Chemical Engineering‡ Department of Materials Science and Engineering and Active Polymer Center for Pattern Integration Yonsei University, Seoul 120-749, Korea§ Material Research Laboratory and Departments of Materials, Chemistry and Biochemistry, University of California, Santa Barbara, California 93016⊥ Department of Polymer Science & Engineering, University of Massachusetts, Amherst, Massachusetts 01003*To whom correspondence should be addressed. E-mail [email protected]Cite this: Macromolecules 2009, 42, 13, 4902–4906Publication Date (Web):May 4, 2009Publication History Received18 January 2009Revised2 April 2009Published online4 May 2009Published inissue 14 July 2009https://pubs.acs.org/doi/10.1021/ma900110whttps://doi.org/10.1021/ma900110wbrief-reportACS PublicationsCopyright © 2009 American Chemical SocietyRequest reuse permissionsArticle Views2657Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Copolymers,Scattering,Surface interactions,Thickness,Thin films Get e-Alerts
A bio-inspired high-authority actuator for shape morphing structures (2003)

Lightweight structures capable of changing their shape on demand are of interest for a number of applications, including aerospace, power generation, and undersea vehicles. This paper describes a bio-inspired cellular metal vertebrate structure which relies on shape memory alloy (SMA) faces to achieve fully reversing shape change. The resulting vertebrate actuators can be combined with flexible face sheets to create a load-bearing, shape morphing panel. Performance of the vertebrate actuator in terms of maximum curvature and moment is analyzed and discussed. A recently constructed, prototype shape morphing airfoil is used to illustrate the concept.
Prediction of the vibration level when monitoring rolling element bearings in paper mill machines (2001)

Rolling element bearings in paper mill machines are considered in this paper due to their critical role in the machine function. In general, the cost-effectiveness of a vibration-based maintenance ...
Advances in size-exclusion separations of proteins and polymers by UHPLC (2014)

The separation of molecular compounds based on their capacity to access the intra-particle pore volume of chromatographic media, which is dictated by the relative size in solution of those compounds, has been commonly known as size-exclusion chromatography (SEC) or gel-permeation chromatography (GPC). Conventionally, these two terms have been applied to the analysis of biomolecules and polymers, respectively. Over the more than half-a-century history of size-based separations, there has been a series of advancements, starting from the earliest soft-gel particles and culminating within the past few years in the use of sub-2-µm particles in ultra-high-performance liquid chromatography (UHPLC). The intent of this review is to provide a concise synopsis of the advancements of both chromatography columns and instrumentation for protein and polymer size-based separations. Also, this review presents brief summaries of the application of UHPLC technology for these classes of analytes.
Analysis of the F Antigen-Specific <i>papA</i> Alleles of Extraintestinal Pathogenic <i>Escherichia coli</i> Using a Novel Multiplex PCR-Based Assay (2000)

Polymorphisms in PapA, the major structural subunit and antigenic determinant of P fimbriae of extraintestinal pathogenic Escherichia coli, are of considerable epidemiological, phylogenetic, and immunotherapeutic importance. However, to date, no method other than DNA sequencing has been generally available for their detection. In the present study, we developed and rigorously validated a novel PCR-based assay for the 11 recognized variants of papA and then used the new assay to assess the prevalence, phylogenetic distribution, and bacteriological associations of the papA alleles among 75 E. coli isolates from patients with urosepsis. In comparison with conventional F serotyping, the assay was extremely sensitive and specific, evidence that papA sequences are highly conserved within each of the traditionally recognized F serotypes despite the diversity observed among F types. In certain strains, the assay detected serologically occult copies of papA, of which some were shown to represent false-negative serological results and others were shown to represent the presence of nonfunctional pap fragments. Among the urosepsis isolates, the assay revealed considerable segregation of papA alleles according to O:K:H serotype, consistent with vertical transmission within clones, but with exceptions which strongly suggested horizontal transfer of papA alleles between lineages. Sequencing of papA from two strains that were papA positive by probe and PCR but F negative in the new PCR assay led to the discovery of two novel papA variants, one of which was actually more prevalent among the urosepsis isolates than were several of the known papA alleles. These findings provide novel insights into the papA alleles of extraintestinal pathogenic E. coli and indicate that the F PCR assay represents a versatile new molecular tool for epidemiological and phylogenetic investigations which should make rapid, specific detection of papA alleles available to any laboratory with PCR capability.
ENHANCING PERSON ANNOTATION FOR PERSONAL PHOTO MANAGEMENT USING CONTENT AND CONTEXT BASED TECHNOLOGIES By (2008)

DUBLIN CITY UNIVERSITYI hereby certify that this material, which I now submit for assessment on the programme of study leading to the award of Doctor of Philosophy, is entirely my own work and has not been taken from the work of others save and to the extent that such work has been cited and acknowledged within the text of my work. Signed: ID number:
ULTRA LOW NOx CATALYTIC COMBUSTION FOR IGCC POWER PLANTS (2004)

Tests were performed in PCI's sub-scale high-pressure (10 atm) test rig, using PCI's two-stage (catalytic / gas-phase) combustion process for syngas fuel. In this process, the first stage is a Rich-Catalytic Lean-burn (RCL{trademark}) catalytic reactor, wherein a fuel-rich mixture contacts the catalyst and reacts while final and excess combustion air cool the catalyst. The second stage is a gas-phase combustor, wherein the catalyst cooling air mixes with the catalytic reactor effluent to provide for final gas-phase burnout and dilution to fuel-lean combustion products. During the reporting period, PCI successfully achieved NOx = 0.011 lbs/MMBtu at 10 atm pressure (corresponding to 2.0 ppm NOx corrected to 15% O{sub 2} dry) with near-zero CO emissions, surpassing the project goal of < 0.03 lbs/MMBtu NOx. These emissions levels were achieved at scaled (10 atm, sub-scale) baseload conditions corresponding to Tampa Electric's Polk Power Station operation on 100% syngas (no co-firing of natural gas).
Gene Therapy for Chronic Relapsing Experimental Allergic Encephalomyelitis Using Cells Expressing a Novel Soluble p75 Dimeric TNF Receptor (2000)

In a murine relapsing experimental allergic encephalomyelitis (EAE) model, gene therapy to block TNF was investigated with the use of a retroviral dimeric p75 TNF receptor (dTNFR) construct. To effectively produce these TNF inhibitors in vivo, a conditionally immortalized syngeneic fibroblast line was established, using a temperature-sensitive SV40 large T Ag-expressing retrovirus. These cells were subsequently infected with a retrovirus expressing soluble dTNFR. CNS-injected cells could be detected 3 mo after transplantation and were shown to produce the transgene product by immunocytochemistry and ELISA of tissue fluids. These levels of dTNFR protein were biologically active and could significantly ameliorate both acute and relapsing EAE. This cell-based gene-vector approach is ideal for delivering proteins to the CNS and has particular relevance to the control of inflammatory CNS disease.
Load Balancing across ECUs in Automotives (2009)

Modern automotive subsystems are no longer a heap of electro-mechanics but instead involve a number of electronic control units (ECUs). Electronic control unit (ECU) has become the fundamental building block of any automotive subsystem and is interfaced with electro mechanics counterpart. To meet the system wide requirements, these ECUs are interconnected using the communication infrastructure. Although the communication infrastructure in terms of, predominantly, the CAN based vehicle network took its birth to enable ECUs to work in a coordinated manner in order to support system wide requirements, during the past decade, this infrastructure was also viewed as a potential means to incorporate extensibility in terms of addition of newer ECUs which are built for implementing additional requirements. With this paradigm, the number of ECUs started growing in a steep manner, uncontrolled and as a result, today, it is not hard to see a high segment automotive housing ECUs as large as 75-80. Hence, load balancing mechanisms are needed to ease ECU integration and for efficient utilization of CPU power in ECUs. In this paper, we explain the concept of load balancing on the basis of CPU utilization across ECUs.
Bandwidth Optimization for Compact Fourier Interpolation (2008)

In this paper, we present a bandwidth-optimization technique for Compact Fourier Interpolation (COMFI). COMFI is a minimum mean-square-error interpolation technique for data sampled at irregular locations. A particular application of interpolation is the estimation of data on a global, Cartesian grid (regularization). The sampling space can be of arbitrary dimension. The interpolated data are computed as a weighted sum of the actual data in a neighbourhood of the selected interpolation locations. The interpolation operator depends on the actual sampling locations and the interpolation location, but not on the data themselves. The operator is designed to have the minimum mean-square interpolation error among all linear operators over a suitable class of spatially band-limited basis functions. We consider, however, the spatial bandwidth as a parameter to be selected in an optimum way. Typically, we compute the COMFI operator to have the largest bandwidth that the actual sampling regime supports, in the sense that the mean-square interpolation error is below an acceptable threshold. We show that the quality of the operator can be strongly dependent on the bandwidth used for its design, and that the optimum bandwidth can vary significantly with spatial location.
Note on the Design of “Terminal-Analog” Speech Synthesizers (1957)

The synthesis of vowel sounds by lumped-constant electrical networks having transfer functions similar to the transmission properties of the vocal tract is considered. A comparison is made between cascade and parallel connections of simple electrical resonators for producing vowel sounds. The cascade arrangement of resonators is shown to yield vowel sounds having formants of proper amplitude when information specifying the formant frequencies only is known.
<i>Staphylococcus aureus</i> Degrades Neutrophil Extracellular Traps to Promote Immune Cell Death (2013)

Bacterial invasion of host tissues triggers polymorphonuclear leukocytes to release DNA [neutrophil extracellular traps (NETs)], thereby immobilizing microbes for subsequent clearance by innate defenses including macrophage phagocytosis. We report here that Staphylococcus aureus escapes these defenses by converting NETs to deoxyadenosine, which triggers the caspase-3-mediated death of immune cells. Conversion of NETs to deoxyadenosine requires two enzymes, nuclease and adenosine synthase, that are secreted by S. aureus and are necessary for the exclusion of macrophages from staphylococcal abscesses. Thus, the pathogenesis of S. aureus infections has evolved to anticipate host defenses and to repurpose them for the destruction of the immune system.
Electromagnetically driven microvalve fabricated in silicon (2002)

This paper reports on a microvalve designed for the use with gaseous media and fabricated in silicon. The focus is on the functional part, the membrane plus valve seat. The membrane to switch the valve between open and closed position is designed having a low spring constant. So only little of the available force is needed for deflection and most of it remains for switching against the load (air pressure). This is shown by FEM simulation (ANSYS 5.2, 5.3). Due to the special design of the valve, packaging as a standalone device as well as full integration into a microsystem is possible.
Dissociating β-Amyloid from α7 Nicotinic Acetylcholine Receptor by a Novel Therapeutic Agent, S 24795, Normalizes α7 Nicotinic Acetylcholine and NMDA Receptor Function in Alzheimer's Disease Brain (2009)

Alzheimer's disease (AD) is characterized by synaptic dysfunction and cardinal neuropathological features including amyloid plaques and neurofibrillary tangles. Soluble amyloid-beta (Abeta) can suppress synaptic activities by interacting with alpha7 nicotinic acetylcholine receptors (alpha7nAChRs). Here, we show that alpha7nAChR and NMDA glutamatergic receptor (NMDAR) activities are severely compromised in synaptosomes prepared from AD and Abeta(1-42) (Abeta42)-exposed control frontal cortex slices from postmortem tissue. Whereas Abeta(12-28) prevents Abeta42 from binding to alpha7nAChRs, 2-[2-(4-bromophenyl)-2-oxoethyl]-1-methyl pyridinium (S 24795), a novel alpha7nAChR partial agonist, facilitates release of Abeta42 from Abeta42-alpha7nAChR and -Abeta42 complexes. S 24795 interacts with alpha7nAChR and Abeta(15-20) region of the Abeta42 to enable partial recovery of the alpha7nAChR and NMDAR channel function. These findings suggest that the Abeta-alpha7nAChR interaction may be an upstream pathogenic event in AD and demonstrate that some recovery of neuronal channel activities may be achieved in AD brains by removing Abeta from alpha7nAChRs.
Isosteric Compounds. I. Acyl Derivatives of Dibenzothiophene (1938)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIsosteric Compounds. I. Acyl Derivatives of DibenzothiopheneAlfred Burger, William B. Wartman Jr., and Robert E. LutzCite this: J. Am. Chem. Soc. 1938, 60, 11, 2628–2630Publication Date (Print):November 1, 1938Publication History Published online1 May 2002Published inissue 1 November 1938https://pubs.acs.org/doi/10.1021/ja01278a023https://doi.org/10.1021/ja01278a023research-articleACS PublicationsRequest reuse permissionsArticle Views139Altmetric-Citations17LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Pharmacokinetic evaluation of recombinant, activated factor VII in patients with inherited factor VII deficiency. (2001)

These findings suggest that the kinetics of rFVIIa are not dose-dependent. In the absence of FVII, the changes of VdArea and CL may be in agreement with a mechanism of competition between FVII and rFVIIa for tissue factor binding.
Cellular manganese content is developmentally regulated in human dopaminergic neurons (2014)

Manganese (Mn) is both an essential biological cofactor and neurotoxicant. Disruption of Mn biology in the basal ganglia has been implicated in the pathogenesis of neurodegenerative disorders, such as parkinsonism and Huntington's disease. Handling of other essential metals (e.g. iron and zinc) occurs via complex intracellular signaling networks that link metal detection and transport systems. However, beyond several non-selective transporters, little is known about the intracellular processes regulating neuronal Mn homeostasis. We hypothesized that small molecules that modulate intracellular Mn could provide insight into cell-level Mn regulatory mechanisms. We performed a high throughput screen of 40,167 small molecules for modifiers of cellular Mn content in a mouse striatal neuron cell line. Following stringent validation assays and chemical informatics, we obtained a chemical 'toolbox' of 41 small molecules with diverse structure-activity relationships that can alter intracellular Mn levels under biologically relevant Mn exposures. We utilized this toolbox to test for differential regulation of Mn handling in human floor-plate lineage dopaminergic neurons, a lineage especially vulnerable to environmental Mn exposure. We report differential Mn accumulation between developmental stages and stage-specific differences in the Mn-altering activity of individual small molecules. This work demonstrates cell-level regulation of Mn content across neuronal differentiation.