gebhart/patrepeval huggingface dataset:
Potential therapeutic role of microRNA in lung cancer treatment (2008)

AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA

SY31-01 

MicroRNAs (miRNAs) are regulatory RNAs found in multicellular eukaryotes, including humans where they are implicated in cancer . miRNAs control gene expression through complementary elements in the 3’UTRs of target mRNAs. The let-7 microRNA is phylogenetically conserved and temporally expressed in many animals. C. elegans let-7 controls terminal differentiation in a stem cell-like lineage in the skin, while human let-7 has been implicated in lung cancer. Four closely related miRNAs constitute the C. elegans let-7 family. To elucidate let-7 ’s role in temporal control of nematode development, we used sequence analysis and reverse genetics to identify candidate let-7 target genes . Additionally we found that one let-7 family member, mir-84 , is expressed dynamically in vulval precursor cells at the time that they are specified for distinct roles in vulval morphogenesis. Specifically, mir-84 is absent when let-60/ras is actively specifying the 1 vulval fate in P6.p. The 3’UTR of let-60/ras contains multiple mir-84 miRNA complementary sites, and restricts reporter gene expression to P6.p. mir-84 over-expression suppresses the multivulva phenotype of activating let-60/ras mutations. This suggests that let-60/ras is a target of mir-84 , which modulates ras signaling leading to vulval cell fate specification. In addition, mammalian HRAS , KRAS and NRAS 3’UTRs contain multiple let-7 complementary sites, suggesting conservation of gene interactions . The 3’ UTRs of the human RAS genes contain multiple LCSs, allowing let-7 to regulate RAS expression. let-7 expression is lower in lung tumors than in normal lung tissue while RAS protein is significantly higher in lung tumors , providing a possible mechanism for let-7 in cancer. The RAS family makes up major oncogenes in various cancers, including lung cancer. We showed that let-7 is highly expressed in lung tissue and that inhibiting let-7 function leads to increased cell division in A549 lung cancer cells, providing evidence that let-7 functions as a tumor suppressor in lung cells . Over-expression of let-7 in cancer cell lines alters cell cycle progression and reduces cell division. let-7 was previously shown to regulate the expression of the RAS lung cancer oncogenes. We also showed that multiple genes involved in cell cycle and cell division functions are also directly or indirectly repressed by let-7. This work reveals the let-7 microRNA to be a master regulator of cell proliferation pathways. This work also suggests that the level of expression of the let-7 miRNA might be an important factor in limiting or contributing to oncogenesis. This may also be the first step in a possible cancer therapy, targeting RAS with a microRNA. This work was supported by a grant from the Connecticut Department of Public Health and from Asuragen, Inc.
Efficient Regulation of VEGF Expression by Promoter-Targeted Lentiviral shRNAs Based on Epigenetic Mechanism (2009)

We show that lentivirus-mediated delivery of shRNA molecules targeted to specific regions in the mVEGF-A promoter either induce or repress VEGF-A expression via epigenetic modulation. Thus, we describe a new approach of gene therapy, epigenetherapy, based on an epigenetic mechanism at the promoter level. Controlling transcription through manipulation of specific epigenetic marks provides a novel approach for the treatment of several diseases.
A mechanism for TCP performance enhancement over asymmetrical environment (2004)

This paper describes a novel mechanism for achieving sufficient TCP performance over some asymmetrical environment without any modification in customer premises. In today's Internet, several types of access media have an asymmetrical bandwidth characteristic, where the downstream link bandwidth is larger than the upstream. However, such asymmetrical environment may cause TCP performance degradation due to upstream link congestion. In order to solve this problem, we propose a PEP (performance enhancement proxy) based approach to improve TCP downloading throughput by reducing upstream traffic using our compulsory IP fragmentation technique. The results of our performance evaluation show that our experimental proxy implementation is capable of accelerating TCP throughput above three times faster comparing to the case without the proxy.
The effect of euthanasia technique on vascular arachidonic acid metabolism and vascular and intestinal smooth muscle contractility. (1990)

This study was designed to determine the effects that specific euthanasia methods have on vascular arachidonic acid metabolism and vascular and intestinal smooth muscle contractility. Rats were euthanatized by decapitation (DC), pentobarbital overdose (PB), or anesthesia with CO2, methoxyflurane or ether followed by DC (CO2-DC, Met-DC, Ether-DC, respectively). Rabbits were killed by a similar protocol, but CO2 overexposure replaced Ether-DC. The rat and rabbit aortas produced mainly 6-keto PGF1 alpha, the prostacyclin metabolite, and lesser amounts of PGE2. No qualitative differences were seen in arachidonate metabolites. However, aortic tissue from rabbits and rats killed by Met-DC produced more prostacyclin. In contrast, aorta from rabbits euthanatized by CO2-DC produced less prostacyclin than controls, whereas aorta from rats killed in the same way yielded greater amounts of prostacyclin. Aortic tissue from rabbits killed by Met-DC and CO2-OD was less responsive to acetylcholine (ACH). Intestinal contractility to ACH was increased in rabbits when Met-DC was used as the method of euthanasia, while colon from rats sacrificed by Met-DC showed decreased responsiveness to ACH. Colon from rats killed by intraperitoneal PB exhibited altered contractility to ACH and norepinephrine. The results of this study show that methoxyflurane, carbon dioxide (rabbit) and pentobarbital (rat) alter the vascular synthesis of prostacyclin and smooth muscle contractility. We conclude that the method of euthanasia affects certain physiologic parameters and careful consideration should be given to the selection of a particular euthanasia technique.
Controlled Nucleation of GaN Nanowires Grown with Molecular Beam Epitaxy (2010)

Abstract The location of GaN nanowires is controlled with essentially perfect selectivity using patterned SiN x prior to molecular beam epitaxy growth. Nanowire growth is uniform within mask openings and absent on the mask surface for over 95% of the usable area of a 76 mm diameter substrate. The diameters of the resulting nanowires are controlled by the size of the mask openings. Openings of approximately 500 nm or less produce single nanowires with symmetrically faceted tips.
Rotorcraft Aerodynamics Models for a Comprehensive Analysis (1998)

Recent developments of the aerodynamics models for the comprehensive analysis CAMRAD II are described, particularly the unsteady aerodynamic models and dynamic stall models, and the free wake geometry calculation. Three models for the unsteady aerodynamic loads in attached flow are implemented: from incompressible thin-airfoil theory, from ONERA EDLIN, and from Leishman-Beddoes. Five dynamic stall models are implemented: from Johnson, Boeing, Leishman-Beddoes, ONERA EDLIN, and ONERA BH. A key feature of the implementation of these models is revisions allowing the retention of airfoil tables for static loads in all cases. Results are presented for a two-dimensional airfoil, a three-dimensional wing, and rotors. Extensions of the CAMRAD II free wake method to include hover and ground effect are described, including hover performance correlation. Notation. A rotor disk area, πR2 cdz,cmz section drag and moment coefficients at zero lift cl,cd,cm section lift, drag, and moment coefficients cn section normal force coefficient CL wind axis rotor lift force, L /ρA(ΩR)2 CP rotor power, P /ρA(ΩR)3 CT rotor thrust, T /ρA(ΩR)2 CX wind axis rotor drag force, X /ρA(ΩR)2 D wake geometry distortion f trailing-edge separation point (fraction chord from leading edge) k reduced frequency, frequency × semichord/speed
Studies on Polymorphism of Monoglycerides. VIII. (1979)

Mechanism for the formation of a solid solution and its modification phenomenon were examined in the binary systems of C18-C16, C18-C14, C18-C12, and C16-C14, using differential scanning colorimetry (DSC) and infrared (IR) spectroscopy.1) Binary systems of C18-C16, C18-C14, and C16-C14 form continuous solid solution in either metastable state or stable state. At transition, they transit from sub-α and α-type solids to β-type solids, and this behavior was similar to that shown by 1-monoglyceride alone. C18-C12 system does not form a solid solution, and C18 and C12 were found to undergo transition independently.2) Phase diagrams of C18-C16, C18-C14, and C16-C14 systems showed a similar pattern. Transition curve from sub-α to α-type was characteristic, the lowest temperature appeared at a specific composition ratio of the two components, and this temperature differed according to the composition ratio of the two camponents. Since C18 and C12 did not dissolve with each other, transition curves of C18 and C12 were different curves.3) Transition rate was affected by the molecular chain length comprising the binary system, and became slower in the order of C18-C16, C18-C14, and C16-C14. The transition rate was the fastest in the case of short-chain monoglyceride containing 510% of longchain monoglyceride, but the rate becomes slower as the transition temperature from α to β-type becomes higher, so that, in general, the rate becomes faster with larger amount of short-chain monoglyceride contained in the long-chain monoglyceride, and became slower in the case of contrary.
Air-Stable n-Doped Colloidal HgS Quantum Dots (2014)

HgS nanocrystals show a strong mid-infrared absorption and a bleach of the near-infrared band edge, both tunable in energy and reversibly controlled by exposure to solution ions under ambient conditions. The same effects are obtained by applying a reducing electrochemical potential, confirming that the mid-infrared absorption is the intraband transition of the quantum dot. This is the first time that stable carriers are present in the quantum state of strongly confined quantum dot in ambient conditions. The mechanism by which doping is achieved is attributed to the rigid shifts of the valence and conduction band with respect to the environment, similar to the sensitivity of the work function of surfaces to adsorbates.
Global Music Asset Assurance Digital Currency : A DRM Solution for Streaming Content Using Blockchain (2018)

The amount of piracy in the streaming digital content in general and the music industry in specific is posing a real challenge to digital content owners. This paper presents a DRM solution to monetizing, tracking and controlling online streaming content cross platforms for IP enabled devices. The paper benefits from the current advances in Blockchain and cryptocurrencies. Specifically, the paper presents a Global Music Asset Assurance (GoMAA) digital currency and presents the iMediaStreams Blockchain to enable the secure dissemination and tracking of the streamed content. The proposed solution provides the data owner the ability to control the flow of information even after it has been released by creating a secure, selfinstalled, cross platform reader located on the digital content file header. The proposed system provides the content owners' options to manage their digital information (audio, video, speech, etc.), including the tracking of the most consumed segments, once it is release. The system benefits from token distribution between the content owner (Music Bands), the content distributer (Online Radio Stations) and the content consumer(Fans) on the system blockchain.
High Prevalence of Glycopeptide Resistance Genes <i>vanB</i> , <i>vanD</i> , and <i>vanG</i> Not Associated with Enterococci in Human Fecal Flora (2005)

ABSTRACT The presence of Enterococcus -associated vancomycin resistance genes vanA , vanB , vanD , vanE , and vanG in rectal swabs was investigated in two hospitals using PCR. All vanA genes detected were associated with vancomycin-resistant enterococci (VRE), whereas VRE-associated vanB genes were detected in only one hospital (4.7%). However, in both hospitals, high prevalences of vanB (6.2 and 2.3%), vanD (43.8 and 26.7%), and vanG (10.5 and 6.9%) genes not associated with enterococci were found.
Automatic I/O hint generation through speculative execution (1999)

Aggressive prefetching is an effective technique for reducing the execution times of disk-bound applications; that is, applications that manipulate data too large or too infrequently used to be found in file or disk caches. While automatic prefetching approaches based on static analysis or historical access patterns are effective for some workloads, they are not as effective as manually-driven (programmer-inserted) prefetching for applications with irregular or input-dependent access patterns. In this paper, we propose to exploit whatever processor cycles are left idle while an application is stalled on I/O by using these cycles to dynamically analyze the application and predict its future I/O accesses. Our approach is to speculatively pre-execute the application’s code in order to discover and issue hints for its future read accesses. Coupled with an aggressive hint-driven prefetching system, this automatic approach could be applied to arbitrary applications, and should be particularly effective for those with irregular and, up to a point, input-dependent access patterns.
Enhanced sensitivity to IGF-II signaling links loss of imprinting of <i>IGF2</i> to increased cell proliferation and tumor risk (2007)

Loss of imprinting (LOI) of the insulin-like growth factor-II gene (IGF2), leading to abnormal activation of the normally silent maternal allele, is a common human epigenetic population variant associated with a 5-fold increased frequency of colorectal neoplasia. Here, we show first that LOI leads specifically to increased expression of proliferation-related genes in mouse intestinal crypts. Surprisingly, LOI(+) mice also have enhanced sensitivity to IGF-II signaling, not simply increased IGF-II levels, because in vivo blockade with NVP-AEW541, a specific inhibitor of the IGF-II signaling receptor, showed reduction of proliferation-related gene expression to levels half that seen in LOI(-) mice. Signal transduction assays in microfluidic chips confirmed this enhanced sensitivity with marked augmentation of Akt/PKB signaling in LOI(+) cells at low doses of IGF-II, which was reduced in the presence of the inhibitor to levels below those found in LOI(-) cells, and was associated with increased expression of the IGF1 and insulin receptor genes. We exploited this increased IGF-II sensitivity to develop an in vivo chemopreventive strategy using the azoxymethane (AOM) mutagenesis model. LOI(+) mice treated with AOM showed a 60% increase in premalignant aberrant crypt foci (ACF) formation over LOI(-) mice. In vivo IGF-II blockade with NVP-AEW541 abrogated this effect, reducing ACF to a level 30% lower even than found in exposed LOI(-) mice. Thus, LOI increases cancer risk in a counterintuitive way, by increasing the sensitivity of the IGF-II signaling pathway itself, providing a previously undescribed epigenetic chemoprevention strategy in which cells with LOI are "IGF-II addicted" and undergo reduced tumorigenesis in the colon upon IGF-II pathway blockade.
Linezolid Therapy of Vancomycin-Resistant <i>Enterococcus faecium</i> Experimental Endocarditis (2001)

We compared the activities of linezolid (25 mg/kg of body weight, administered intraperitoneally every 8 h) and of vancomycin (25 mg/kg of body weight, administered intraperitoneally every 8 h) in a rat model of vanA vancomycin-resistant Enterococcus faecium experimental endocarditis. Results were expressed as median log(10) CFU per gram of vegetation after 3 days of treatment. The median log(10) CFU per gram of vegetation was 10.1 among 7 untreated control animals, 10.2 among 9 vancomycin-treated animals, and 7.9 among 10 linezolid-treated animals. Linezolid treatment was more active (P < 0.05) than vancomycin treatment or no treatment.
Purification and identification of lactoperoxidase in milk basic proteins as an inhibitor of osteoclastogenesis (2011)

A milk protein fraction with alkaline isoelectric points (milk basic protein, MBP) inhibits both bone resorption and osteoclastogenesis for in vitro models. We previously identified bovine angiogenin as a component of MBP that inhibits bone resorption. However, purified angiogenin had no effect on osteoclastogenesis, suggesting that MBP contains unidentified component(s) that inhibit osteoclast formation. In this study, we purified lactoperoxidase (LPO) as the predominant inhibitor of osteoclastogenesis in MBP. The LPO treatment downregulated levels of reactive oxygen species in osteoclasts. Signaling by receptor activator of NF-kappa-B ligand/receptor activator of NF-kappa-B (RANKL/RANK) was downregulated in LPO-treated cells, and, in particular, the ubiquitination of tumor necrosis factor receptor associate factor 6 (TRAF6) and activation of downstream signaling cascades (JNK, p38, ERK, and NFκB) were suppressed. Ultimately, LPO treatment led to decreased expression of c-Fos and NFAT2. These results suggest that MBP contains at least 2 components that independently suppress bone resorption through a unique mechanism: angiogenin inhibits bone resorption and LPO inhibits RANKL-induced osteoclast differentiation. These data explain many of the positive aspects of milk consumption on bone health.
Network-based application-independent time-error and direct port latency measurement (2016)

A system is proposed by which a link partner on a path with known latency and asymmetry can directly observe the time error of the attached slave PTP device, including offset from master as well as ingress and egress latency on the port. A qualified system implementing the proposed solution can provide application-independent real-time network based monitoring to assess a time sensitive networks / cyber-physical system's quality of time at the monitored slave nodes.
The alkene monooxygenase from <i>Xanthobacter</i> Py2 is a binuclear non‐haem iron protein closely related to toluene 4‐monooxygenase (1998)

The genes encoding the six polypeptide components of the alkene monooxygenase from Xanthobacter Py2 have been sequenced. The predicted amino acid sequence of the first ORF shows homology with the iron binding subunits of binuclear non-haem iron containing monooxygenases including benzene monooxygenase, toluene 4-monooxygenase (> 60% sequence similarity) and methane monooxygenase (> 40% sequence similarity) and that the necessary sequence motifs associated with iron co-ordination are also present. Secondary structure prediction based on the amino acid sequence showed that the predominantly alpha-helical structure that surrounds the binuclear iron binding site was conserved allowing the sequence to be modelled on the co-ordinates of the methane monooxygenase alpha-subunit. Significant differences in the residues forming the hydrophobic cavity which forms the substrate binding site are discussed with reference to the differences in reaction specificity and stereospecificity of binuclear non-haem iron monooxygenases.
Multi-dipolar Chromophores Featuring Phosphine Oxide as Joint Acceptor: A New Strategy toward High-Efficiency Blue Thermally Activated Delayed Fluorescence Dyes (2016)

Blue thermally activated delayed fluorescence (TADF) dyes are basically combinations of strong acceptors and weak donors. In this work, a weak acceptor P═O group was employed to construct a series of weak acceptor–strong donor (WASD)-type emitters with a phenoxazine donor, namely 10-(4-(diphenylphosphoryl)phenyl)-10H-phenoxazine (SPXZPO), 10,10′-(4,4′-(phenylphosphoryl)bis(4,1-phenylene))bis(10H-phenoxazine) (DPXZPO), and 10,10′,10″-(4,4′,4″-phosphoryltris(benzene-4,1-diyl))tris(10H-phenoxazine) (TPXZPO). Owing to the insulating effect of P═O on conjugation extension and intramolecular electronic communications, the photoluminescence spectra of these molecules are almost identical, manifesting the superiority of WASD structure in emission color preservation. Simultaneously, the multi-dipolar characteristics of TPXZPO enhance the intramolecular charge transfer (ICT), facilitating reverse intersystem crossing for higher TADF efficiency and shorter lifetime. As a consequence, TPXZPO realized the desired pure-blue electroluminescence peak at 464 nm, accompanied by a favorable external quantum efficiency (ηext) up to 15.3%, 100% exciton utilization, and reduced efficiency roll-offs. Its complementary full-TADF white organic light-emitting diodes also achieved ηext as high as 16.3%, among the best results reported so far for white TADF devices. The success of TPXZPO, the first example of a P═O-based WASD-type blue TADF dye, is attributed to the comprehensive and harmonized effects of the P═O joint on controlling conjugation and intramolecular electronic communication and the multi-dipolar structure on enhancing ICT.
Identification and localization of extracellular Ca<sup>2+</sup>-sensing receptor in rat intestine (1998)

The extracellular calcium (Ca2+o)-sensing receptor (CaR) plays vital roles in Ca2+o homeostasis, but no data are available on its expression in small and large intestine. Polymerase chain reaction products amplified from reverse-transcribed duodenal RNA using CaR-specific primers showed > 99% homology with the rat kidney CaR. Northern analysis with a CaR-specific cRNA probe demonstrated 4.1- and 7.5-kb transcripts in all intestinal segments. Immunohistochemistry with CaR-specific antisera showed clear basal staining of epithelial cells of small intestinal villi and crypts and modest apical staining of the former, whereas there was both basal and apical staining of colonic crypt epithelial cells. In situ hybridization and immunohistochemistry also demonstrated CaR expression in Auerbach's myenteric plexus of small and large intestines and in the submucosa in the region of Meissner's plexus. Our results reveal CaR expression in several cell types of small and large intestine, in which it may modulate absorptive and/or secretomotor functions.
Effect of different dialkylamino groups on the regioselectivity of lithiation of O-protected 3-(dialkylamino)phenols (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEffect of different dialkylamino groups on the regioselectivity of lithiation of O-protected 3-(dialkylamino)phenolsMaria Skowronska-Ptasinska, Willem Verboom, and David N. ReinhoudtCite this: J. Org. Chem. 1985, 50, 15, 2690–2698Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/jo00215a020https://doi.org/10.1021/jo00215a020research-articleACS PublicationsRequest reuse permissionsArticle Views1044Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts