gebhart/patrepeval huggingface dataset:
Post-endocytic Sorting of Calcitonin Receptor-like Receptor and Receptor Activity-modifying Protein 1 (2007)

Calcitonin receptor-like receptor (CLR) and the receptor activity-modifying protein 1 (RAMP1) comprise a receptor for calcitonin gene-related peptide (CGRP). Although CGRP induces endocytosis of CLR/RAMP1, little is known about post-endocytic sorting of these proteins. We observed that the duration of stimulation with CGRP markedly affected post-endocytic sorting of CLR/RAMP1. In HEK and SK-N-MC cells, transient stimulation (10(-7) M CGRP, 1 h), induced CLR/RAMP1 recycling with similar kinetics (2-6 h), demonstrated by labeling receptors in living cells with antibodies to extracellular epitopes. Recycling of CLR/RAMP1 correlated with resensitization of CGRP-induced increases in [Ca(2+)](i). Cycloheximide did not affect resensitization, but bafilomycin A(1), an inhibitor of vacuolar H(+)-ATPases, abolished resensitization. Recycling CLR and RAMP1 were detected in endosomes containing Rab4a and Rab11a, and expression of GTPase-defective Rab4aS22N and Rab11aS25N inhibited resensitization. After sustained stimulation (10(-7) M CGRP, >2 h), CLR/RAMP1 trafficked to lysosomes. RAMP1 was degraded approximately 4-fold more rapidly than CLR (RAMP1, 45% degradation, 5 h; CLR, 54% degradation, 16 h), determined by Western blotting. Inhibitors of lysosomal, but not proteasomal, proteases prevented degradation. Sustained stimulation did not induce detectable mono- or polyubiquitination of CLR or RAMP1, determined by immunoprecipitation and Western blotting. Moreover, a RAMP1 mutant lacking the only intracellular lysine (RAMP1K142R) internalized and was degraded normally. Thus, after transient stimulation with CGRP, CLR and RAMP1 traffic from endosomes to the plasma membrane, which mediates resensitization. After sustained stimulation, CLR and RAMP1 traffic from endosomes to lysosomes by ubiquitin-independent mechanisms, where they are degraded at different rates.
The <i>apbE</i> Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in <i>Salmonella typhimurium</i> (1998)

Thiamine pyrophosphate is an essential cofactor that is synthesized de novo in Salmonella typhimurium. The biochemical steps and gene products involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine have yet to be elucidated. We have isolated mutations in a new locus (Escherichia coli open reading frame designation yojK) at 49 min on the S. typhimurium chromosome. Two significant phenotypes associated with lesions in this locus (apbE) were identified. First, apbE purF double mutants require thiamine, specifically the HMP moiety. Second, in the presence of adenine, apbE single mutants require thiamine, specifically both the HMP and the thiazole moieties. Together, the phenotypes associated with apbE mutants suggest that flux through the purine pathway has a role in regulating synthesis of the thiazole moiety of thiamine and are consistent with ApbE being involved in the conversion of AIR to HMP. The product of the apbE gene was found to be a 36-kDa membrane-associated lipoprotein, making it the second membrane protein implicated in thiamine synthesis.
A simple rapid purification scheme for hydroxymethylbilane synthase from human erythrocytes (1988)

Hydroxymethylbilane synthase from human erythrocytes was purified 47,000-fold to greater than 95% homogeneity and 7.5% yield by a simple and rapid procedure using heat treatment (80 degrees C, in the presence of proteinase inhibitors, to convert one of two chromatographically separable forms into the other), DEAE-cellulose and Cibacron Blue F3G-A-Sepharose chromatographies and Sephadex G-75 gel filtration. The purified enzyme was similar to the enzyme purified from other species in showing hyperbolic dependence of velocity on substrate concentration, a non-linear progress curve for uroporphyrinogen appearance, and was monomeric, having an Mr of 44,000 by gel filtration on Sephadex G-100 and h.p.l.c. and an Mr of 45,000 on SDS/polyacrylamide-gel electrophoresis. The enzyme showed a sharp pH profile for Vmax, and various folates were shown to accelerate neither the enzymic formation of hydroxymethylbilane nor ring-closure of hydroxymethylbilane.
Pyrene–Oxadiazoles for Organic Light-Emitting Diodes: Triplet to Singlet Energy Transfer and Role of Hole-Injection/Hole-Blocking Materials (2015)

Three pyrene-oxadiazole derivatives were synthesized and characterized by optical, electrochemical, thermal, and theoretical investigations to obtain efficient multifunctional organic light emitting diode (OLED) materials. Synthesized molecules were used as emitters and electron transporters in three different device configurations, involving hole-injection/hole-blocking materials that showed good current and power efficiencies. To understand the underlying mechanisms involved in the application of these molecules as emitters and transporters, a detailed photophysical characterization of molecules 4-6 was carried out. The absorption, steady-state fluorescence, phosphorescence, fluorescence lifetime, and phosphorescence lifetime measurements were carried out. The high quantum yield and efficient reverse intersystem crossing leading to delayed fluorescence emission makes the molecule a good emitter, and the charge delocalization properties leading to excimer formation make them efficient electron transporters. Isoenergetic singlet and triplet states of the molecules make the reverse intersystem crossing feasible at room temperature even in the absence of thermal activation.
Virtual damping and einstein relation in oscillators (2003)

This paper presents a new physical theory of oscillator phase noise. Built around the concept of phase diffusion, this work bridges the fundamental physics of noise and existing oscillator phase-noise theories. The virtual damping of an ensemble of oscillators is introduced as a measure of phase noise. The explanation of linewidth compression through virtual damping provides a unified view of resonators and oscillators. The direct correspondence between phase noise and the Einstein relation is demonstrated, which reveals the underlying physics of phase noise. The validity of the new approach is confirmed by consistent experimental agreement.
Agile photonic fractional Fourier transformation of optical and RF signals (2017)

International audience
Colored-noise-induced first-order phase transition in a single-mode dye laser (1987)

We report the observation of a colored-noise-induced first-order nonequilibrium phase transition in a single-mode dye laser. Although a second-order phase transition exists in the more usual single-mode laser models, the order of the phase transition can be altered by including colored-noise fluctuations in the pumping of the laser. The nonwhite character of the noise is shown to be crucial to the onset of the characteristics of a first-order phase transition.
The GRASP Taxonomy of Human Grasp Types (2015)

In this paper, we analyze and compare existing human grasp taxonomies and synthesize them into a single new taxonomy (dubbed “The GRASP Taxonomy” after the GRASP project funded by the European Commission). We consider only static and stable grasps performed by one hand. The goal is to extract the largest set of different grasps that were referenced in the literature and arrange them in a systematic way. The taxonomy provides a common terminology to define human hand configurations and is important in many domains such as human-computer interaction and tangible user interfaces where an understanding of the human is basis for a proper interface. Overall, 33 different grasp types are found and arranged into the GRASP taxonomy. Within the taxonomy, grasps are arranged according to 1) opposition type, 2) the virtual finger assignments, 3) type in terms of power, precision, or intermediate grasp, and 4) the position of the thumb. The resulting taxonomy incorporates all grasps found in the reviewed taxonomies that complied with the grasp definition. We also show that due to the nature of the classification, the 33 grasp types might be reduced to a set of 17 more general grasps if only the hand configuration is considered without the object shape/size.
Schema evolution in object databases by catalogs (2002)

We are concerned with schema evolution in object oriented databases (OODB) that is processed by a modification of the classes of the schema. We present a new categorization of the different modifications in three categories: primitive, composite and complex modifications. On this basis, we propose a method by decomposition for addressing conceptual schema evolution: "real-life" complex schema modifications are solved by decomposition into simpler well known and controlled modifications, called composites, which in turn may be reduced to so-called primitives. A key step in making this approach practical, is the provision of two catalogs: one for the primitives based on a sound object model and one for the composites built on top of the primitive catalog. Such catalogs raise the level of abstraction, further reuse and are prerequisites for effective tool support. We define the three types of schema modifications, describe these catalogs, put them into the context of our decomposition approach and provide a process for schema evolution.
Synthesis and Structure−Phytotoxicity Relationships of Acetylenic Phenols and Chromene Metabolites, and Their Analogues, from the Grapevine Pathogen <i>Eutypa </i><i>l</i><i>ata</i> (2002)

Eutypa lata, the fungus responsible for dying-arm disease in grapevines, produces a number of structurally related secondary metabolites, of which eutypine (1) has been implicated as the principal phytotoxin. However, analysis of an E. lata strain from California known to be pathogenic to grapevines showed that eutypine was not present, suggesting that other metabolites could be phytotoxic. Investigation of the relative phytotoxicities of individual metabolites has been limited by insufficient material and lack of a reliable bioassay. Metabolites of particular interest and their precursors were therefore synthesized, and a rapid, quantitative bioassay via topical application of individual compounds to disks of grape leaves and measurement of chlorophyll loss was developed to provide a relative measure of tissue damage. The recently reported metabolite eulatachromene (2) was found to have phytotoxicity greater than that of eutypine (1). The cyclization product, 5-formyl-2-methylvinyl[1]benzofuran (3), also showed significant activity, whereas the reduction product, eutypinol (4), was inactive, as was the quinol, siccayne (5). These results indicate that before strains of Eutypa are incriminated as pathogenic they must be analyzed for the presence or absence of specific constituents for which the phytotoxicity has been unequivocally established.
Role of the Multidrug Efflux Systems of <i>Pseudomonas aeruginosa</i> in Organic Solvent Tolerance (1998)

Multidrug efflux pumps with a broad substrate specificity make a major contribution to intrinsic and acquired multiple antibiotic resistance in Pseudomonas aeruginosa. Using genetically defined efflux pump mutants, we investigated the involvement of the three known efflux systems, MexA-MexB-OprM, MexC-MexD-OprJ, and MexE-MexF-OprN, in organic solvent tolerance in this organism. Our results showed that all three systems are capable of providing some level of tolerance to organic solvents such as n-hexane and p-xylene. Expression of MexAB-OprM was correlated with the highest levels of tolerance, and indeed, this efflux system was a major contributor to the intrinsic solvent tolerance of P. aeruginosa. Intrinsic organic solvent tolerance was compromised by a protonophore, indicating that it is substantially energy dependent. These data suggest that the efflux of organic solvents is a factor in the tolerance of P. aeruginosa to these compounds and that the multidrug efflux systems of this organism can accommodate organic solvents, as well as antibiotics.
Investigational drugs for eating disorders (2003)

AbstractThe eating disorders anorexia nervosa, bulimia nervosa and binge eating disorder are common, significant public health problems which are treated with nutritional, psychotherapeutic and pharmacological interventions. A number of drugs (mostly antidepressant drugs) are currently used in their treatment to some benefit, but there is substantial room for improvement. A wide variety of compounds are listed as under investigation for the treatment of eating disorders. They have a diverse variety of mechanisms of action, reflecting the complex nature of the control of food intake. While none of these compounds are close to release at present, the diversity of mechanisms under study lend some optimism that more effective approaches will be identified.Keywordsanorexia nervosabinge eating disorderbulimia nervosaeating disorderserotonin
Establishment and Use of a General Practitioner Medical Unit in a District General Hospital (1972)

An analysis of the establishment and running of a general-practitioner medical unit in a district general hospital has shown that it fulfills a useful and positive role in the area health services and is well used. It shows that selectivity of admission can and does work and that the theoretical drawbacks to such a unit did not materialize in practice during the study.
Effects of Kojic acid, Arbutin and Vitamin C on cell viability and melanin synthesis in B16BL6 cells (2003)

Melanin biosynthesis is a human defense mechanism to protect skin from UV irradiation and also determines colors of hair and skin. However, as a interest on skin-whitening increases, researches to prevent pigmentation and hypersynthesis of melanin in skin are being actively in progress. Active components used as a whitening agent in cosmeceuticals are kojic acid, arbutin, vitamin C and hydroquinone. However, until now, because comparison researches among them in the aspect of both melanin formation and cellular toxicity have not been performed, we can't exactly estimate merits and defects of them as a whitening agent. To this end, we performed experiments to compare their effects on cell viability and melanin formation. As a first step, in vitro tyrosinase inhibition assay was done. While kojic acid and hydroquinone showed strong inhibition activities(their IC50/s are all < 100uM), arbutin and vitamin C showed weak activities. IC50/s of arbutin and vitamin C are 100uM and 400∼500uM, respectively.
In B16BL6 melanoma cells, like in vitro tyrosinase inhibition assay, arbutin and kojic acid showed more strong inhibition effect on melanin synthesis than vitamin C. And unlike arbutin, vitamin C and kojic acid induced cell death at high concentration. Although arbutin showed no cytotoxicity, it has side effect to induce morphological change at high concentration.. In this paper, we suggest both kojic acid and arbutin have stronger ability to inhibit melanogenesis than vitamin C. And they also have side effect, that is, kojic acid induces cell death like vitamin C and arbutin changes cell morphology respectively.
The Components of the Plants of Lagerstroemia Genus. V. Synthesis of the Aglycon of Lagertannin 3, 4-Di-O-methylellagic Acid from Gallic Acid (1977)

The aglycon of lagertannin, 3, 4-di-O-methylellagic acid (I), was synthesized from gallic acid and methyl 3-O-methylgallate (II) which was obtained in a fairly good yield by one-step method according to the two layer procedure of Bredereck.
Advanced glycation end product receptor interactions on microvascular cells occur within caveolin‐rich membrane domains (2000)

Advanced glycation end products (AGEs) have an important role in diabetic complications, with many responses mediated through AGE-receptors. The current study has investigated the binding and uptake of AGEs by retinal microvascular endothelium in an attempt to understand the nature of AGE-interaction with receptors on the cell surface. There has been special emphasis placed on the R1, R2, and R3 components of AGE-receptor complex (AGE-RC) and their localization to caveolin-rich membrane domains. Retinal microvascular endothelial cells (RMECs) were exposed to either AGE-modified BSA (AGE-BSA) or native BSA conjugated to colloidal gold (gAGE, gBSA) for various time periods, fixed, and processed for transmission electron microscopy (TEM). Localization of AGE-RC components in caveolae was investigated using confocal microscopy and ultrastructural immunogold labeling. Caveolae were extracted from RMECs using differential Triton X-100 solubility, and Western analysis was conducted to test for caveolae enrichment and the presence of AGE-RC complex components. Ligand blots determined 125I-AGE-BSA binding to caveolae-enriched extracts. Colloidal gold conjugates of AGE-BSA bound to caveolae and were internalized to be trafficked to lysosomal-like compartments. AGE-receptor complex components were significantly enriched within caveolae. The data suggest that AGEs interact with their receptors within caveolae. It is significant that the AGE-R complex localizes to these organelles, because this may have implications for AGE binding, internalization, signal transduction, and the modulation of AGE-receptor-mediated vascular cell dysfunction.
An 8-bit, 16 input, 3.2 pJ/op switched-capacitor dot product circuit in 28-nm FDSOI CMOS (2016)

An 8-bit, 16 input, switched-capacitor dot product circuit in 28-nm FDSOI CMOS is presented. The design uses sixteen 8-bit passive charge redistribution digital-to-analog multipliers followed by an 8-bit SAR ADC. Measured energy per dot product operation is 3.2 pJ. When used to compute partial dot products for the hidden layer of a three-layer neural network, the design achieves a classification accuracy of 98.0% on the MNIST handwritten digit dataset.
Activity-Based Probe for Histidine Kinase Signaling (2012)

Bacterial two-component systems (TCSs) are signaling pathways composed of two proteins: a histidine kinase (HK) and a response regulator (RR). Upon stimulation, the HK autophosphorylates at a conserved histidine. The phosphoryl group is subsequently transferred to an aspartate on an RR, eliciting an adaptive response, often up- or downregulation of gene expression. TCS signaling controls many functions in bacteria, including development, virulence, and antibiotic resistance, making the proteins involved in these systems potential therapeutic targets. Efficient methods for the profiling of HKs are currently lacking. For direct readout of HK activity, we sought to design a probe that enables detection of the phosphotransfer event; however, analysis of the phosphohistidine species is made difficult by the instability of the P-N bond. We anticipated that use of a γ-thiophosphorylated ATP analogue, which would yield a thiophosphorylated histidine intermediate, could overcome this challenge. We determined that the fluorophore-conjugated probe, BODIPY-FL-ATPγS, labels active HK proteins and is competitive for the ATP binding site. This activity-based probe provides a new strategy for analysis of TCSs and other HK-mediated processes and will facilitate both functional studies and inhibitor identification.
Are Commercially Available Essential Oils from Australian Native Plants Repellent to Mosquitoes? (2009)

While the use of topical insect repellents, particularly those containing synthetic active ingredients such as deet (N,N-diethyl-3-methylbenzamide), are a mainstay in personal protection strategies emphasized in public health messages, there is a growing demand in the community for alternative repellents, particularly those of botanical origin and thus deemed to be "natural." This study evaluated the repellency of essential oils from 11 Australian native plants in 5% v/v formulations against Aedes aegypti, Culex quinquefasciatus, and Culex annulirostris under laboratory conditions. A blend of the top 3 performing oils was then compared with deet and a commercially available botanical insect repellent. All essential oils provided at least some protection against the 3 mosquito species, with the longest protection time (110 min) afforded by Prostanthera melissifolia against Cx. quinquefasciatus. Mean protection times against Ae. aegypti were substantially lower than those for the Culex spp. tested. Deet provided significantly longer protection against Ae. aegypti than both the 5% v/v blend of Leptospermum petersonii, Prostanthera melissifolia, and Melaleuca alternifolia (the 3 most effective oils) and the commercial botanical repellent. The results of this study indicate that these essential oils from Australian native plants offer limited protection against biting mosquitoes and that a blend of essential oils holds may offer commercial potential as a short-period repellent or under conditions of low mosquito abundance. However, it is important that public health messages continue to emphasize the greater effectiveness of deet-based repellents in areas with risks of mosquito-borne disease.
A Sensitivity-Enhancing Control Approach for Structural Model Updating (2007)

Model updating plays an important role in structural design and dynamic analysis. The process of model updating aims to produce an improved mathematical model by correlating the initial model with the experimentally measured data. There are a variety of techniques available for model updating using dynamic and static measurements of the structure’s behavior. This paper focuses on the model updating methods using the measured natural frequencies of the structure. The practice of model updating using only the natural frequencies encounters two well-known limitations: deficiency of frequency measurement data, and low sensitivity of measured natural frequencies with respect to the physical parameters that need to be updated. To overcome these limitations, a novel model updating method is presented in this paper. First, closed-loop control is applied to the structure to enhance the sensitivity of natural frequencies to the updating parameters. Second, by including the natural frequencies based on a series of sensitivity-enhanced closed-loop systems, we can significantly enrich the frequency measurement data available for model updating. Using the natural frequencies of these sensitivity-enhanced closed-loop systems, an iterative process is utilized to update the physical parameters in the initial model. To demonstrate and verify the proposed method, case studies are carried out using a cantilevered beam structure. The natural frequencies of a series of sensitivity-enhanced closed-loop systems are utilized to update the mass and stiffness parameters in the initial FE model. Results show that the modeling errors in the mass and stiffness parameters can be accurately identified by using the proposed model updating method.
Achieving service portability in ICEBERG (2002)

There is a growing trend toward service access through heterogeneous devices from diverse networks. It thus becomes crucial to provide support for mobility and portability at the application and service level to enable seamless service access from any end devices and access networks. Such support allows transparent roaming and ubiquitous service access. In the ICEBERG project (Wang et al. 2000), our goal is to develop such a service infrastructure to integrate a variety of telephony and data services spanning diverse access networks reaching heterogeneous end users. In this paper, we discuss our techniques for achieving the goals of personal and service mobility, transparent network- and device-independent service access, as well as highly scalable and fault-tolerant access to composed service entities across a wide area. We evaluate our implementation through applications such as Universal In-box, Interactive Voice Room Control, MP3-Jukebox access using a cell-phone, and realtime video delivery to wireless clients.