gebhart/patrepeval huggingface dataset:
Molecular evolution of the growth hormone-releasing hormone/pituitary adenylate cyclase-activating polypeptide gene family. Functional implication in the regulation of growth hormone secretion (2000)

Growth hormone-releasing hormone (GHRH) and pituitary adenylate cyclase-activating polypeptide (PACAP) belong to the same superfamily of regulatory neuropeptides and have both been characterized on the basis of their hypophysiotropic activities. This review describes the molecular evolution of the GHRH/PACAP gene family from urochordates to mammals and presents the hypothesis that the respective roles of GHRH and PACAP in the control of GH secretion are totally inverted in phylogenetically distant groups of vertebrates. In mammals, GHRH and PACAP originate from distinct precursors whereas, in all submammalian taxa investigated so far, including birds, amphibians and fish, a single precursor encompasses a GHRH-like peptide and PACAP. In mammals, GHRH-containing neurons are confined to the infundibular and dorsomedial nuclei of the hypothalamus while PACAP-producing neurons are widely distributed in hypothalamic and extrahypothalamic areas. In fish, both GHRH- and PACAP-immunoreactive neurons are restricted to the diencephalon and directly innervate the adenohypophysis. In mammals and birds, GHRH plays a predominant role in the control of GH secretion. In amphibians, both GHRH and PACAP are potent stimulators of GH release. In fish, PACAP strongly activates GH release whereas GHRH has little or no effect on GH secretion. The GHRH/PACAP family of peptides thus provides a unique model in which to investigate the structural and functional facets of evolution.
Cognitive & Communicative Principle and the Interpretation of Bridging Anaphora (2013)

Bridging anaphora, a usual phenomenon in daily communication, is characterized by the anaphoric expressions which are definite noun phrases. This process can be interpreted as the search for relevance by communicators. Cognitive Principle claims that humans intuitively concentrate their minds on relevance and Communicative Principle provides bases for identifying the referents. Bridging anaphora can be expounded cognitively under the framework of Relevance Theory.
Investigation of Compressive Strength of Molded Cylinders and Drilled Cores of Concrete (1961)

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Restraining add-on's behavior in private browsing (2012)

In this paper we address the privacy issues of add-on mechanism supported by browser in private mode. The add-ons enjoy unrestrained access to user sensitive information at all times. This freedom can be misused to create add-ons with malicious intent of violating privacy of the browser. We have designed and implemented an add-on which performs this task in private mode of the browser. This is a clear violation of the goals of private browsing. Mozilla lacks privacy ensuring mechanism against add-ons at browser level. So we have modified the source code of Mozilla Firefox to prevent such behavior of an add-on. It involves runtime monitoring of add-on's behavior in private mode and notify/block suspicious ones. We have been able to prevent such add-on's activity using our mechanism.
Regio- and Stereoselectivity in the Coupling Reaction of Secologanin with Dopamine Derivatives (2001)

The coupling reaction of tetraacetylsecologanin with dopamine and its N-benzyl derivative was investigated. In both series, stereoisomers at C-1, as well as regioisomer normal and neo compounds, were formed. Moreover, the N-unsubstituted products were partially lactamized, and the N-benzyl derivatives epimerized at C-1. In the products, the R configuration of C-1 over the S and the formation of the normal structure over the neo one predominated. The epimerization of both epimers gave an equilibrium of R and S in a ratio of 7:3 and was interpreted by cleavage of the C-1-N-2 bond. The fact that lactamization was much faster in the R than in the S series was explained on the basis of the supposed transition states. The structure, the configuration of C-1, and in several cases the conformations were established by detailed NMR studies and supported by chemical correlations.
Table Look-Up MOSFET Modeling System Using a 2-D Device Simulator and Monotonic Piecewise Cubic Interpolation (1983)

This paper describes a method for connecting an MOSFET 2-D device simulator to a circuit simulator via a 3-D table look-up MOSFET model. The computational cost of the device simulator is drastically reduced by a proposed monotonic piecewise cubic interpolation technique. With this technique, the device simulator needs to calculate only 100 ~ 200 points to make up an accurate 3-D table look-up MOSFET model. The computational time necessary for the interpolation is only about one third of the time for calculating one current point by the device simulator.
Structural Geology (2010)

Lavishly illustrated in color, this textbook takes an applied approach to introduce undergraduate students to the basic principles of structural geology. The book provides unique links to industry applications in the upper crust, including petroleum and groundwater geology, which highlight the importance of structural geology in exploration and exploitation of petroleum and water resources. Topics range from faults and fractures forming near the surface to shear zones and folds of the deep crust. Students are engaged through examples and parallels drawn from practical everyday situations, enabling them to connect theory with practice. Containing numerous end-of-chapter problems, e-learning modules, and with stunning field photos and illustrations, this book provides the ultimate learning experience for all students of structural geology.
Distinct functions of T- and L-type calcium channels during activation of bovine adrenal glomerulosa cells. (1996)

Calcium influx into adrenal glomerulosa cells is a key event during the stimulation of aldosterone secretion by physiological increases in extracellular potassium concentrations. Two types of voltage-operated calcium channels, T- and L-types, are present on bovine glomerulosa cells, but their respective functions are not yet clearly defined. Using the patch-clamp method in the perforated patch configuration combined with microfluorimetry of cytosolic calcium, we demonstrate that L-type channels are exclusively responsible for the sustained elevation of cytosolic calcium observed upon stimulation with extracellular potassium, even at low, physiological concentrations of this agonist. In contrast, aldosterone secretion appears closely related to T-type channel activity. Moreover, when the activity of each channel type is selectively modulated by pharmacological agents, such as dihydropyridines or zonisamide, the cytosolic calcium response can be clearly dissociated from the steroidogenic response. Similarly, modulation of T channel activation by protein kinase C results in a parallel inhibition of aldosterone secretion, without any effect on the levels of cytosolic free calcium. This direct functional link between T-type calcium channel activity and steroidogenesis suggests a model in which calcium entering the cell through these channels bypasses the cytosol to activate intramitochondrial steps of aldosterone biosynthesis.
250V 1A three-phase monolithic inverter IC for brushless motor drives. (1991)

This paper describes a high voltage three-phase monolithic inverter IC using dielectrically isolated power IC technology. This inverter IC has the ability to drive a 50 W brushless motor, and it can be supplied voltage from a commercial voltage source using only a rectifier. This inverter IC includes six insulated gate bipolar transistors (IGBTs) and fast recovery diodes for output stages having a current capability of 1 A for continuous current. The IGBTs and fast recovery diodes realize a reduction in forward voltage drops and fast switching speeds. The IC also provides functions such as drive circuits suitable for pulse width modulation (PWM) control at a switching frequency of 20 kHz, a charge pump type power supply for each upper driver, and unique overcur rent protection circuits on one chip. The drive circuits which prevent the IGBTs from incorrect turning on under the large dV/dt condition, and the overcurrent circuits, provide easy use and enhanced reliability.
Continuous nondestructive monitoring of microbial biofilms: A review of analytical techniques (1995)

A fundamental requirement for the understanding and control of biofilms is the continuous nondestructive monitoring of biofilm processes. This paper reviews research analytical techniques that monitor biofilm processes in a continuous nondestructive manner and that could also be modified for industrial applications. To be considered 'continuous' and 'nondestructive' for the purpose of this review a technique must: (a) function in an aqueous system; (b)not require sample removal; (c) minimize signal from organisms or contaminants in the bulk phase; and (d) provide real-time data. Various microscopic, spectrochemical, electrochemical, and piezoelectrical analysis methods fulfill these criteria. These techniques monitor the formation of biofilms, the physiology of the microorganisms withiin biofilms, and/or the interaction of the biofilms with their environment. It is hoped that this review will stimulate development and use of biofilm monitoring techniques in industrial and environmental settings.
Antifungal activity of the extracts and saponins from Sapindus saponaria L. (2007)

Extracts from the dried pericarp of Sapindus saponaria L. (Sapindaceae) fruits were investigated for their antifungal activity against clinical isolates of yeasts Candida albicans and C. non-albicans from vaginal secretions of women with Vulvovaginal Candidiasis. Four clinical isolates of C. albicans, a single clinical isolated of each of the species C. parapsilosis, C. glabrata, C. tropicalis, and the strain of C. albicans ATCC 90028 were used. The hydroalcoholic extract was bioactivity-directed against a clinical isolate of C. parapsilosis, and showed strong activity. The n-BuOH extract and one fraction showed strong activity against all isolates tested. Further column-chromatography on silica gel separation of this fraction afforded two pure triterpene acetylated saponins: 3-O-(4-acetyl-beta-D-xylopyranosyl)-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabinopyranosyl-hederagenin (1) and 3-O-(3,4-di-acetyl-beta-D-xylopyranosyl)-(1->3)-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabynopyranosyl-hederagenin (2). The structures of the compounds were based on spectral data ((1)H and 13C NMR, HSQC, HMBC and MS), and on with literature. The saponins isolated showed strong activity against C. parapsilosis.
<i>FGFR3</i> and <i>Tp53</i> Mutations in T1G3 Transitional Bladder Carcinomas: Independent Distribution and Lack of Association with Prognosis (2005)

FGFR3 and Tp53 mutations have been proposed as defining two alternative pathways in the pathogenesis of transitional bladder cancer. FGFR3 mutations are associated with low-grade tumors and a favorable prognosis. Tp53 alterations are associated with advanced tumors and, possibly, with a poor prognosis. We focus here on the subgroup of T1G3 superficial tumors because they are a major clinical challenge. Patients (n = 119) were identified from a prospective study of 1,356 cases. Mutations in FGFR3 (exons 7, 10, and 15) and Tp53 (exons 4-9) were analyzed using PCR and direct sequencing. All cases were followed for recurrence and death. Survival was analyzed using Kaplan-Meier curves and multivariable Cox regression. FGFR3 mutations were detected in 20 (16.8%) tumors; 100 mutations in Tp53 were found in tumors from 78 (65.5%) cases. Multiple alterations in Tp53 were present in 19 tumors (16%). Inactivating mutations were present in 58% of tumors. The combined mutation distribution (FGFR3/Tp53) was: wt/wt (34.5%), mut/wt (7.6%), wt/mut (48.7%), and mut/mut (9.2%), indicating that the presence of either mutation did not depend on the other (P value = 0.767). FGFR3 and Tp53 mutations were not associated with clinicopathologic characteristics of patients and did not predict, alone or in combination, recurrence or survival. Taking the risk of the wt/wt group as reference, the mutation-associated risks of cancer-specific mortality were: mut/wt 1.42 (0.15-13.75), wt/mut 0.67 (0.19-2.31), mut/mut 1.62 (0.27-9.59). These molecular features support the notion that T1G3 tumors are at the crossroads of the two main molecular pathways proposed for bladder cancer development and progression.
Brain Lactic Acidosis and Ischemic Cell Damage: 2. Histopathology (1981)

The influence of severe tissue lactic acidosis during incomplete brain ischemia (30 min) on cortex morphology was studied in fasted rats. Production of lactate in the ischemic tissue was varied by preischemic infusions (i.v.) of either a saline or a glucose solution. The brains were fixed by perfusion with glutaraldehyde at 0, 5, or 90 min of recirculation. In saline-infused animals (tissue lactate about 15 μmol g −1 ), changes observed at 0 and 5 min of recirculation were strikingly discrete: slight condensation of nuclear chromatin. mild to moderate mitochondrial swelling, and only slight astrocyte edema. These changes had virtually disappeared after 90 min recirculation and. at this time. only discrete ribosomal changes were observed. In contrast. glucose-infused rats (tissue lactate about 35 μmol g −1 ) showed severe changes: marked clumping of nuclear chromatin and cell sap in all cells was already evident at 0 and 5 min recirculation, while mitochondrial swelling was mild to moderate. Although tissue fixation was inadequate at 90 min. the ultrastructural appearance indicated extensive damage. It is concluded that excessive tissue lactic acidosis during brain ischemia exaggerates structural alterations and leads to irreversible cellular damage. A tentative explanation is offered for the paucity (<0.2%) of condensed neurons with grossly swollen mitochondria. previously considered a hallmark of ischemic cell injury.
Sorry Dave, I'm Afraid I Can't Do That: Explaining Unachievable Robot Tasks Using Natural Language (2013)

This paper addresses the challenge of enabling nonexpert users to command robots to perform complex high-level tasks using natural language. It describes an integrated system that combines the power of formal methods with the accessibility of natural language, providing correct-by-construction controllers for high-level specifications that can be implemented, and easy-to-understand feedback to the user on those that cannot be achieved. This is among the first works to close this feedback loop, enabling users to interact with the robot in order to identify a succinct cause of failure and obtain the desired controller. The supported language and logical capabilities are illustrated using examples involving a robot assistant in a hospital.
Biodegradation of <i>Bombyx mori</i> silk fibroin fibers and films (2003)

Abstract The in vitro biodegradation of Bombyx mori silk fibroin was studied by incubating fibers and films with proteolytic enzymes (collagenase type F, α‐chymotrypsin type I‐S, protease type XXI), for times ranging from 1 to 17 days. The changes in sample weight and degree of polymerization of silk fibers exposed to proteolytic attack were negligible. However, tensile properties were significantly affected, as shown by the drop of strength and elongation as a function of the degradation time. Upon incubation with proteolytic enzymes, silk films exhibited a noticeable decrease of sample weight and degree of polymerization, the extent of which depended on the type of enzyme, on the enzyme‐to‐substrate ratio, and on the degradation time. Protease was more aggressive than α‐chymotrypsin or collagenase. Film fragments resistant to enzymatic degradation were enriched in glycine and alanine. FT‐IR measurements showed that the degree of crystallinity of biodegraded films increased. Soluble degradation products of silk films consisted of a range of peptides widely differing in size, deriving from the amorphous sequences of the silk fibroin chains. Biodegraded fibers showed an increase of surface roughness, while films displayed surface cracks and cavities with internal voids separated by fiber‐like elements. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 91: 2383–2390, 2004
Single Point Mutations Distributed in 10 Soluble and Membrane Regions of the Nicotiana plumbaginifolia Plasma Membrane PMA2 H+-ATPase Activate the Enzyme and Modify the Structure of the C-terminal Region (1998)

The Nicotiana plumbaginifolia pma2 (plasma membrane H+-ATPase) gene is capable of functionally replacing the H+-ATPase genes of the yeast Saccharomyces cerevisiae, provided that the external pH is kept above 5.0. Single point mutations within the pma2 gene were previously identified that improved H+-ATPase activity and allowed yeast growth at pH 4.0. The aim of the present study was to identify most of the PMA2 positions, the mutation of which would lead to improved growth and to determine whether all these mutations result in similar enzymatic and structural modifications. We selected additional mutants in total 42 distinct point mutations localized in 30 codons. They were distributed in 10 soluble and membrane regions of the enzyme. Most mutant PMA2 H+-ATPases were characterized by a higher specific activity, lower inhibition by ADP, and lower stimulation by lysophosphatidylcholine than wild-type PMA2. The mutants thus seem to be constitutively activated. Partial tryptic digestion and immunodetection showed that the PMA2 mutants had a conformational change making the C-terminal region more accessible. These data therefore support the hypothesis that point mutations in various H+-ATPase parts displace the inhibitory C-terminal region, resulting in enzyme activation. The high density of mutations within the first half of the C-terminal region suggests that this part is involved in the interaction between the inhibitory C-terminal region and the rest of the enzyme.
Surface Plasmon Resonance Biosensor Technology for Marine Toxin Analysis (2014)

Marine biotoxins are diverse chemical compounds that are classified according to the acute symptoms that they present to humans and other mammals upon their ingestion within contaminated seafood. Biosensor technologies are analytical tools for the recognition and measurement of a target through its association with a biological component and physiochemical detector. Commercial surface plasmon resonance (SPR) biosensors such as Biacore systems consist of three key components: the optical detector at the SPR interface, an interchangeable chip surface onto the optical interface, and an integrated micro uidic system with liquid handling systems operated by computer-driven software. A number of different types of binders have been exploited in the development of various methodologies for the detection of marine biotoxins. Antibodies unlike receptors are biochemical binders and bind selectively to chemical features on the structure of the toxin to which they are produced. Polyclonal antibodies tend to display better sensitivity due to multiple epitope binding.
Generation of Artificial Proteoglycans Containing Glycosaminoglycan-modified CD44 (1999)

All CD44 isoforms are modified with chondroitin sulfate (CS), while only those containing variably spliced exon V3 are modified with both CS and heparan sulfate (HS). The CS is added to a serine-glycine (SG) site in CD44 exon E5, while HS and CS are added to the SGSG site in exon V3. Site-directed mutagenesis and other molecular biology techniques were used to determine the minimal motifs responsible for the addition of CS and HS to CD44 (see accompanying paper (Greenfield, B., Wang, W.-C., Marquardt, H., Piepkorn, M., Wolff, E. A., Aruffo, A., and Bennett, K. L. (1999) J. Biol. Chem. 274, 2511-2517)). We have used this information to generate artificial proteoglycans containing the extracellular domain of the cell adhesion protein lymphocyte function-associated antigen-3 (LFA-3) (CD58) and CD44 motifs modified with CS or a combination of CS and HS. Analysis of the CD44-modified LFA-3 protein showed that it retains the ability to engage and trigger the function of its natural ligand CD2, resulting in T cell activation. In addition, the glycosaminoglycan-modified artificial proteoglycan is capable of binding the chemokine RANTES (regulated upon activation, normally T cell expressed and secreted) and delivering it to human T cells, resulting in enhanced T cell activation. These data demonstrate that artificial proteoglycans can be engineered with functional domains that have enhanced activity by codelivering glycosaminoglycan-binding molecules. The artificial proteoglycans were also used as a model system to explore the glycosaminoglycan binding properties of basic-fibroblast growth factor and the chemokine RANTES. While basic-fibroblast growth factor was shown to bind HS alone, this model revealed that RANTES binds not only HS, as has been demonstrated in the past, but also CS. Thus, artificial proteoglycans can be used for studying the glycosaminoglycan binding patterns of growth factors and chemokines and provide a means to manipulate the levels, types, and activity of glycosaminoglycan-binding proteins in vitro and in vivo.
Mutants of Bacteriophage T4 Defective in the Induction of T4 Endonuclease II (1971)

Abstract Some mutants of bacteriophage T4 selected for their inability to replicate in the presence of hydroxyurea are defective in the degradation of host DNA. Nine such mutants which all map in the same locus (the denA locus) were all deficient in the induction of T4 endonuclease II. We conclude that this enzyme is involved in the breakdown of cellular DNA.
Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase. (1993)

The three-dimensional structure of the hybrid Bacillus 1,3-1,4-beta-glucanase (beta-glucanase; 1,3-1,4-beta-D-glucan 4-glucanohydrolase, lichenase, EC 3.2.1.73) designated H(A16-M) was determined by x-ray crystallography at a resolution of 2.0 A and refined to an R value of 16.4% using stereochemical restraints. The protein molecule consists mainly of two seven-stranded antiparallel beta-pleated sheets arranged atop each other to form a compact, sandwich-like structure. A channel crossing one side of the protein molecule accommodates an inhibitor, 3,4-epoxybutyl beta-D-cellobioside, which binds covalently to the side chain of Glu-105, as seen in a crystal structure analysis at 2.8-A resolution of the protein-inhibitor complex (R = 16.8%). That Glu-105 may be indispensible for enzyme catalysis by H(A16-M) is suggested by site-directed mutagenesis of this residue, which inevitably leads to an inactive enzyme.
Design and Analysis of VTOL Operated Intercity Electrical Vehicle for Urban Air Mobility (2021)

This paper discusses the conceptual design of an intercity electrical vertical take-off-and-landing aircraft. A literature survey of existing eVTOL aircrafts, configuration selection, initial sizing, weight estimation, modelling and analysis was conducted. The present intercity eVTOL aircraft has the capability to carry four passengers along with one pilot for a distance of 500 km. Two specific aircraft modes, such as air-taxi and air-cargo mode, are considered in the present design. Market entry is predicted before 2031. Subsequently, innovative technologies are incorporated into the design. The present design features an aerodynamically shaped fuselage, tapered wing and a V-tail design. It can carry a nominal payload of 500 kg to a maximum range of 500 km at a cruise speed of Mach 0.168. The present eVTOL is comprised of a 5 m-long fuselage and an 11 m wingspan. It utilizes six tilt-rotor propeller engines. The maximum take-off weight and empty weight are 1755 kg and 1255 kg, respectively. The unit price is expected to be between USD 14.83 and 17.36 million. This aircraft has an aesthetically pleasing, intelligent and feasible design.