gebhart/patrepeval huggingface dataset:
A single intravenous injection of oncolytic picornavirus SVV-001 eliminates medulloblastomas in primary tumor-based orthotopic xenograft mouse models (2010)

Difficulties of drug delivery across the blood-brain barrier (BBB) and failure to eliminate cancer stem cells (CSCs) are believed to be the major causes of tumor recurrences in children with medulloblastoma (MB). Seneca Valley virus-001 (SVV-001) is a naturally occurring oncolytic picornavirus that can be systemically administered. Here, we report its antitumor activities against MB cells in a panel of 10 primary tumor-based orthotopic xenograft mouse models. We found that SVV-001 killed the primary cultured xenograft cells, infected and replicated in tumor cells expressing CSC surface marker CD133, and eliminated tumor cells capable of forming neurospheres in vitro in 5 of the 10 xenograft models. We confirmed that SVV-001 could pass through BBB in vivo. A single i.v. injection of SVV-001 in 2 anaplastic MB models led to widespread infection of the preformed intracerebellar (ICb) xenografts, resulting in significant increase in survival (2.2-5.9-fold) in both models and complete elimination of ICb xenografts in 8 of the 10 long-term survivors. Mechanistically, we showed that the intracellular replication of SVV-001 is mediated through a subverted autophagy that is different from the bona fide autophagic process induced by rapamycin. Our data suggest that SVV-001 is well suited for MB treatment. This work expands the current views in the oncolytic therapy field regarding the utility of oncolytic viruses in simultaneous targeting of stem and nonstem tumor cells.
Near-infrared femtosecond laser crystallized poly-Si thin film transistors (2007)

Polycrystalline silicon (poly-Si) thin film transistors (TFTs) fabricated by near-infrared femtosecond laser annealing (FLA) are demonstrated. The FLA-annealed poly-Si channels exhibit low tail-state, deep-state, and midgap-state densities of grain traps. Characteristics such as field-effect mobility, threshold voltage, and subthreshold slope for FLA-annealed poly-TFTs are comparable to those of conventional approaches. A wide process window for annealing laser fluences was confirmed by examining the changes in electrical parameters for transistors with various channel dimensions.
Tips and tricks for stenting of bifurcation coronary lesions. (2010)

Although the provisional stenting technique to treat coronary bifurcation lesions is the preferred method, many bifurcation lesions still require a two-stent technique to optimize the result and clinical outcome. This manuscript summarizes the tips and tricks of bifurcation stenting with cases illustrations. As interventionists are encountering more complex and difficult cases, one may find the tips and tricks in this manuscript helpful in daily practice.
Lung-Specific Transgenic Expression of KC Enhances Resistance to <i>Klebsiella pneumoniae</i> in Mice (1998)

A vigorous host response is required to effectively clear pathogenic bacteria from the lungs and is dependent upon the recruitment and activation of neutrophils and macrophages. A family of chemotactic cytokines, referred to as chemokines, have been shown to participate in this complex protective response. In this study, we assessed the role of the C-X-C chemokine KC in lung antibacterial host defense using wild-type (wt) B6D2 mice or transgenic mice that had been bred on a B6D2 background expressing KC under the control of a Clara cell-specific promoter within the lung. The administration of Klebsiella pneumoniae to both wt and KC-transgenic mice resulted in a time-dependent expression of KC protein within the lung that peaked at 24 to 48 h postinoculation. When infected with K. pneumoniae, the KC-transgenic mice showed a striking improvement in survival compared with wt control mice. This improved survival was due to an increase in bacterial clearance, which occurred in association with a vigorous recruitment of neutrophils in the KC-transgenic mice compared with their wt control counterparts. No differences in the lung levels of the specific cytokines TNF-alpha, IFN-gamma, IL-12, and IL-10 were noted. However, inducible macrophage inflammatory protein-2 levels were significantly decreased in the KC-transgenic mice compared with the wt mice. This study indicates that the compartmentalized overexpression of KC in vivo results in increased lung bacterial clearance and improved survival, which occurs in association with enhanced polymorphonuclear leukocyte influx to the lung.
Wide-band low-loss high-isolation microstrip periodic-stub diplexer for multiple-frequency applications (2001)

This paper introduces a three-port microstrip multifrequency diplexer used in a phased-array transceiver system that employs band-stop filters with open-circuited stubs for band selection and separation. The diplexer is designed to take 10, 12, 19, and 21 GHz into port 1 and to separate 10 and 19 GHz to port 2 and 12 and 21 GHz to part 3 with minimal dispersion. The insertion loss for each frequency varies from 0.4 to 3.4 db and the return loss is better than 10 dB. The isolation between channels at the four frequencies is greater than 50 dB. Each passband created between adjacent stopbands has a bandwidth over 1 GHz. The microstrip diplexer is designed using periodic stubs that collectively have the advantages of low insertion loss, high isolation and rejection, wide-band performance on each channel, and easy fabrication. This type of diplexer has many applications in multifrequency transceivers for communication systems.
Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication (1991)

Replication protein A (RP-A) is a three-subunit single-stranded DNA-binding protein that has been isolated from human cells. RP-A is essential for SV40 DNA replication and may also be important in genetic recombination. The sequence of a cDNA encoding the 70-kDa subunit of human RP-A is reported. The 616-amino acid predicted open reading frame of the human protein is 31% identical with the 621-amino acid open reading frame of the 70-kDa subunit of RP-A from the yeast Saccharomyces cerevisiae. Both proteins share a highly conserved putative metal binding domain of the 4-cysteine type. The human cDNA directs production in Escherichia coli of a 70-kDa protein that reacts with a monoclonal antibody directed against the 70-kDa subunit of human RP-A. The recombinant 70-kDa subunit, purified from bacteria, exhibits single-stranded DNA binding activity comparable to that of the complete RP-A complex. The 70-kDa subunit is able to substitute for the complete human RP-A complex in stimulating the activity of DNA polymerase alpha-primase on a poly(dA).oligo(dT) template. However, the 70-kDa subunit alone cannot substitute for the complete RP-A complex in SV40 DNA replication in vitro, suggesting an important functional role for the other subunits.
Characterization of signals and noise rejection with bipolar longitudinal intrafascicular electrodes (1999)

Longitudinal intrafascicular electrodes (LIFE's) are fine electrodes threaded into the extracellular space between axons in peripheral nerves or spinal roots. We are developing these electrodes for application in functional electrical stimulation and in basic physiology. An area of concern in chronic recording application of LIFE's is the possibility of electromyogram and other external noise sources masking the recorded neural signals. We characterized neural signals recorded by LIFE's and confirmed by three independent methods that increasing interelectrode spacing for bipolar LIFE's increases signal amplitude. The spectrum of neural signal from bipolar and monopolar LIFE lies between 300 Hz and 10 kHz. The amplitude of the spectrum increases with increasing interelectrode spacing, although the distribution is not affected. Single unit analysis of LIFE recordings show that they record selectively from units closest to the electrode active site. Units with conduction velocities ranging from 50-120 m/s were identified. Extraneural noise, as stimulus artifact or electromyogram, is much reduced with bipolar LIFE recording, as compared to monopolar recordings. Relative improvement in neural signal to extraneural noise increases with interelectrode spacing up to about 2 mm. Since there is no further improvement beyond 2 mm, we conclude that the preferred interelectrode spacing for bipolar LIFE's is 2 mm.
MSL2 Promotes Mdm2-independent Cytoplasmic Localization of p53 (2008)

Although it was originally thought of as a passive way to block the nuclear function of p53, accumulating evidence suggests that cytoplasmic localization of p53 plays an active role in p53-mediated functions such as apoptosis and autophagy. Previous studies by us and others demonstrated that Mdm2-mediated p53 ubiquitination induces both degradation and cytoplasmic localization. Here we describe MSL2, a novel E3 ligase for p53 that promotes ubiquitin-dependent cytoplasmic p53 localization. Unlike Mdm2 or most other p53 E3 ligases, MSL2-mediated p53 ubiquitination does not affect the stability of p53. Moreover, the MSL2-mediated effect on p53 is Mdm2-independent. Thus, our study identifies an important ubiquitin-ligase for modulating p53 subcellular localization.
Adaptive Vertex Quantization for Mesh Compression (2007)

Computer-Aided Design and Applications is an international journal on the applications of CAD and CAM. It publishes papers in the general domain of CAD plus in emerging fields like bio-CAD, nano-CAD, soft-CAD, garment-CAD, PLM, PDM, CAD data mining, CAD and the internet, CAD education, genetic algorithms and CAD engines. The journal is aimed at all developers and users of CAD technology to ptovide CAD solutions for various stages of design and manufacturing. The journal publishes all about Computer-Aided Design and Computer-Aided technologies.
Targeting Oxidative Stress and Aberrant Critical Period Plasticity in the Developmental Trajectory to Schizophrenia (2015)

Schizophrenia is a neurodevelopmental disorder reflecting a convergence of genetic risk and early life stress. The slow progression to first psychotic episode represents both a window of vulnerability as well as opportunity for therapeutic intervention. Here, we consider recent neurobiological insight into the cellular and molecular components of developmental critical periods and their vulnerability to redox dysregulation. In particular, the consistent loss of parvalbumin-positive interneuron (PVI) function and their surrounding perineuronal nets (PNNs) as well as myelination in patient brains is consistent with a delayed or extended period of circuit instability. This linkage to critical period triggers (PVI) and brakes (PNN, myelin) implicates mistimed trajectories of brain development in mental illness. Strategically introduced antioxidant treatment or later reinforcement of molecular brakes may then offer a novel prophylactic psychiatry.
Limitations of the X-ray Photoelectron Spectroscopy Technique in the Study of Electroactive Polymers (1997)

Polyaniline (PAN) and polypyrrole (PPY) were analyzed by X-ray photoelectron spectroscopy (XPS) and bulk analytical techniques. A systematic comparison of the results was carried out to determine the effects of anion size, synthesis methods, and the nature of the polymer (powder or film) on the variation between the surface and bulk compositions. Good agreement between the XPS and bulk analytical results is generally obtained for chemically synthesized polyaniline (in powder form). However, these two methods may give substantial discrepancies in the analysis of anion concentration in as-cast polyaniline base films subjected to re-doping by acids. Such discrepancies are also found in the analysis of as-synthesized polypyrrole doped by large anions and polypyrrole base films and powders after re-doping by acids. In these cases the XPS technique tends to give anion concentrations that are significantly higher than the bulk values. In some of these cases the high surface anion concentration supports a high proportion of positively charged nitrogens (>50% for polyaniline, >30% for polypyrrole), which must involve the protonation of some amine units in addition to all of the imine units. However, in actuality, the re-doping process can only replenish the anions to less than half of the original bulk concentration in polypyrrole.
Temperature compensated high-Q dielectric resonators for long term stable low phase noise oscillators (2002)

High temperature superconductor (HTS)-shielded dielectric resonators with Q-values >10/sup 5/ at T=77 K are promising candidates for applications in low phase noise oscillators. We have investigated feedback-oscillators at 5.6 and 10.2 GHz and are currently setting up an oscillator at 23 GHz. To achieve ultimate e-values we have used TE/sub 01/spl delta/-/ and whispering gallery mode resonators with the central dielectric made from sapphire or lanthanum aluminate (LaAlO/sub 3/). Employing commercial MESFET-amplifiers we have determined the phase noise performance of feedback oscillators at these frequencies. These results confirm the superior performance of HTS-shielded dielectric resonator stabilised oscillators. The long term stability of the oscillator is mainly determined by the temperature stability of the resonator frequency. Thermally induced variations of the permittivity E of the central dielectric puck cause frequency drifts of the resonator frequency. Rutile has successfully been used to build resonators with frequency stabilities <0.3 ppm/K at 5.6 GHz. To enable accurate positioning of the oscillator frequency we have investigated the effect of varying the distance between the central dielectric and a superconducting end plate for a whispering gallery mode resonator at 23 GHz. We have also started utilising a Stirling-type miniature cooler to cool the resonators. We present data on the influence of cooler vibrations on the purity of the oscillator frequency.
Measurement of carbon film thickness by inelastic electron scatter (2001)

The electron spectra obtained from magnetic media coated with carbon films of varying thickness are presented. Electrons backscattered inelastically dominate the spectra. The intensity of the elastic peak varies in accordance with the Beer/Lambert law and escape depths of 30 and 43 Å for 1 and 2 keV primary beam energies, respectively. The maximum intensity in the inelastic scatter portion of the spectrum decreases in energy and magnitude with increasing film thickness. The spectral differences associated with changes in carbon film thickness are large and provide an alternative to x-ray photoelectron spectrometry for quantitative carbon film thickness measurement.
Angiotensin‐(1‐7) reduces proliferation and angiogenesis of human prostate cancer xenografts with a decrease in angiogenic factors and an increase in sFlt‐1 (2012)

The decrease in PlGF and VEGF coupled with the increase in sFlt-1 suggests that Ang-(1-7) may serve as a novel anti-angiogenic therapy for prostate cancer. Further, the pleiotropic mechanisms of action by Ang-(1-7) may limit angiogenic resistance that occurs with VEGF inhibitors or receptor blockers.
Concurrent Optimization of Coverage, Capacity, and Load Balance in HetNets Through Soft and Hard Cell Association Parameters (2018)

Ultradense heterogeneous networks (HetNets) are emerging as an inevitable approach to tackle the capacity crunch in cellular networks. However, imbalanced load among small and macrocells and poor resource utilization as a consequence in HetNets remains a long-standing problem. This paper addresses this problem by presenting a solution for maximization of coverage and capacity while minimizing load imbalance among macro and small cells. Most recent studies on the topic focus on either optimization of coverage, capacity or load, or a combination of two of these three intertwined objectives. We formulate the optimization problem as a function of two hard parameters namely antenna tilt and transmit power, and a soft parameter, cell individual offset, that affect the coverage, capacity, and load directly. The resulting solution is a combination of the otherwise conflicting coverage and capacity optimization (CCO) and load balancing (LB) self-organizing network (SON) functions. In the presented joint CCO-LB solution, a conflict free operation of CCO and LB is ensured by designing a novel load aware user association methodology and resolving the effects of shadowing on coverage probability using stochastic approximation. The problem is proven to be nonconvex and is solved using genetic algorithm, sequential quadratic programming, and pattern search algorithms. The proposed CCO-LB solution is compared against two recently proposed CCO and CCO-LB solutions in the literature. Results show that the proposed solution can yield significant gain in terms of throughput, spectral efficiency, and load distribution.
Neural predictors of cognitive improvement by multi-strategic memory training based on metamemory in older adults with subjective memory complaints (2018)

Previous studies have indicated that memory training may help older people improve cognition. However, evidence regarding who will benefit from such memory trainings has not been fully discovered yet. Understanding the clinical and neural inter-individual differences for predicting cognitive improvement is important for maximizing the training efficacy of memory-training programs. The purpose of this study was to find the individual characteristics and brain morphological characteristics that predict cognitive improvement after a multi-strategic memory training based on metamemory concept. Among a total of 49 older adults, 39 participated in the memory-training program and 10 did not. All of them underwent brain MRIs at the entry of the training and received the neuropsychological tests twice, before and after the training. Stepwise regression analysis showed that lower years of education predicted cognitive improvement in the training group. In MRI, thinner cortices of precuneus, cuneus and posterior cingulate gyrus and higher white matter anisotropy of the splenium of corpus callosum predicted cognitive improvement in the training group. Old age, lower education level and individual differences in cortical thickness and white matter microstructure of the episodic memory network may predict outcomes following multi-strategic training.
Atomic energy in industry. (1947)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAtomic energy in industry.I. Perlman Cite this: J. Chem. Educ. 1947, 24, 3, 115Publication Date (Print):March 1, 1947Publication History Received3 August 2009Published online1 March 1947Published inissue 1 March 1947https://doi.org/10.1021/ed024p115RIGHTS & PERMISSIONSArticle Views32Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (5 MB) Get e-Alerts Get e-Alerts
Expression, Purification and Characterization of the Rat Luteal 20α-Hydroxysteroid Dehydrogenase<sup>1</sup> (1997)

The enzyme, rat ovarian 20 alpha-hydroxysteroid dehydrogenase (20 alpha HSD), plays a central role in luteolysis and parturition. It catalyzes the reduction of progesterone, leading to the formation of progestationally inactive steroid, 20 alpha-hydroxypregn-4-ene-3-one (20 alpha-hydroxyprogesterone). Recently, we reported the cloning, sequencing, and deduced amino acid sequence of the rat luteal 20 alpha HSD. To further investigate whether phosphorylation and/or glycosylation affect the activity of 20 alpha HSD and to study its kinetic and biochemical properties, we established both bacterial and insect expression systems for obtaining large quantities of enzyme. The recombinant (rec) 20 alpha HSD expressed as glutathione-S-transferase-20 alpha HSD fusion protein was purified from bacterial lysates by affinity binding to glutathione-Sepharose beads followed by thrombin digestion, whereas the rec enzyme expressed in baculovirus-insect cell system was purified to apparent homogeneity by ion exchange chromatography, followed by dye affinity chromatographies. Both rec preparations of 20 alpha HSD demonstrated a single polypeptide chain of 37 kDa with similar K(m) values for 20 alpha-hydroxyprogesterone and NADP, although the corresponding maximum velocity values were slightly lower for the rec 20 alpha HSD expressed in the insect cells. The rec 20 alpha-HSD showed preference for progesterone/20 alpha-hydroxyprogesterone. 17 alpha-Hydroxyprogesterone was only 30% as effective. The enzyme also used various substrates specific for aldo-keto reductases, although with much less efficiency. The rec enzyme preparations showed an absolute requirement for NADP(H). In vitro phosphorylation of rec bacterial enzyme with either protein kinase A or protein kinase C had no demonstrable effect on its activity. Finally, no differences in enzyme activity were noted between glycosylated (expressed in insect cells) and nonglycosylated (expressed in bacteria) forms of the enzyme. In conclusion, these studies demonstrate that rat luteal 20 alpha HSD can be prepared in large amounts from either bacterial or insect expression systems in a catalytically active form. Indirect evidence also suggests that the catalytic activity of 20 alpha HSD may be independent of phosphorylation and glycosylation states of the enzyme protein, i.e. posttranslational modification of 20 alpha HSD may not be required for the maximal expression of enzyme activity.