gebhart/patrepeval huggingface dataset:
Functional β-propeller lectins by tandem duplications of repetitive units (2010)

Internal symmetry in proteins is likely to be the footprint of evolution by gene duplication and fusion. Like other symmetrical proteins, β-propellers, which are made of 4-10 β-sheet units (blades) circularly arranged around a central tunnel, have probably evolved by duplication and fusion of a rudimentary repetitive unit. However, reproducing the evolution of functional β-propellers by duplication and fusion of repeated units remains a challenge, in particular, because the repeated units must jointly pack to form one hydrophobic core while maintaining intact active sites. As model for generating repeat propellers, we chose tachylectin-2--a highly symmetrical five-bladed β-propeller lectin with five sugar-binding sites. We report the engineering of folded and functional lectins by duplication and fusion of repetitive sequence modules taken from tachylectin-2. The repeated modules comprise three strands of one blade plus one strand of the next blade, thus enabling the closure of the propeller's ring via strand-strand Velcro-like interactions. Duplication and fusion of five modules with the same sequence gave rise to a highly aggregated protein, yet its soluble fraction exhibited lectin function. Subsequently, a library of diversified sequence modules fused in tandem was selected by phage display for glycoprotein binding. A range of new lectins were isolated with binding and biophysical properties that resemble those of wild-type tachylectin-2. These results demonstrate the ability to construct folded and functional globular repeat proteins, and support the role of duplication and fusion of elementary modules in the evolutionary routes that led to the β-propellers fold.
LOBOT: Low-Cost, Self-Contained Localization of Small-Sized Ground Robotic Vehicles (2012)

It is often important to obtain the real-time location of a small-sized ground robotic vehicle when it performs autonomous tasks either indoors or outdoors. We propose and implement LOBOT, a low-cost, self-contained localization system for small-sized ground robotic vehicles. LOBOT provides accurate real-time, 3D positions in both indoor and outdoor environments. Unlike other localization schemes, LOBOT does not require external reference facilities, expensive hardware, careful tuning or strict calibration, and is capable of operating under various indoor and outdoor environments. LOBOT identifies the local relative movement through a set of integrated inexpensive sensors and well corrects the localization drift by infrequent GPS-augmentation. Our empirical experiments in various temporal and spatial scales show that LOBOT keeps the positioning error well under an accepted threshold.
Crosstalk between p53 and FOXO Transcription Factors (2004)

Recent studies find striking similarities between p53 and the FOXO family of forkhead transcription factors. Both transcription factors can be posttranslationally modified, for example, by acetylation and phosphorylation. They regulate a number of genes in common. Upregulation of either p53 or FKHRL1 leads to cell cycle arrest or apoptosis, depending on cellular context and type of stress. While these findings suggest that p53 and FOXO may parallel in their functions, our recent report demonstrates cross talk between these two transcription factors in vivo. Since both p53 and FOXO have been described as potential drug targets in cancer therapy, we believe understanding their functional interactions as well as the signaling pathways involved will be important to direct their clinical application.
Aneuploidy in human spermatozoa: FISH analysis in men with constitutional chromosomal abnormalities, and in infertile men (2001)

Reproductive difficulties are associated intimately with cytogenetic abnormalities. This article reviews multicolour fluorescence in situ hybridization studies on spermatozoa from men with constitutional chromosomal abnormalities and the consequences for spermatozoa, and on chromosomal abnormalities in the spermatozoa of infertile men who have normal somatic karyotypes. In 47,XYY men, the frequencies of 24,XY and 24,YY spermatozoa appear to be < or = 1%. Klinefelter (47,XXY) and mosaic Klinefelter patients had sperm aneuploidy frequencies of 2-25% and 1.5-7.0%, respectively. Robertsonian translocation carriers had 3-27% spermatozoa unbalanced for the chromosomes involved in the translocation, with a possible modest interchromosomal effect, but none of the increased frequencies of chromosomal disomy approached 1%. The frequency of chromosomally unbalanced spermatozoa in reciprocal translocations averages 50%, is strongly dependent on the chromosomes involved in the individual translocation, and may be slightly increased as a result of a small interchromosomal effect. Infertile men with a normal karyotype and low sperm concentration or certain types of morphologically abnormal spermatozoa have a significantly increased risk of producing aneuploid spermatozoa, particularly for the sex chromosomes. An increased risk of sperm aneuploidy was not observed in infertile men with poor sperm motility or in those with a normal karyotype and normal semen parameters.
Dual numerical aperture confocal operation of movingfibre bar code reader (1999)

A compact bar code reader using pre-lens scanning by a piezoelectrically-actuated vibrating optical fibre cantilever is demonstrated. Dual numerical aperture confocal operation, based on transmission of a guided mode and reception of cladding modes, is shown to be a simple method for increasing and separating the return signal.
Two-channel Hyperspectral LiDAR with a Supercontinuum Laser Source (2010)

Recent advances in nonlinear fiber optics and compact pulsed lasers have resulted in creation of broadband directional light sources. These supercontinuum laser sources produce directional broadband light using cascaded nonlinear optical interactions in an optical fibre framework. This system is used to simultaneously measure distance and reflectance to demonstrate a technique capable of distinguishing between a vegetation target and inorganic material using the Normalized Difference Vegetation Index (NDVI) parameters, while the range can be obtained from the waveform of the echoes. A two-channel, spectral range-finding system based on a supercontinuum laser source was used to determine its potential application of distinguishing the NDVI for Norway spruce, a coniferous tree, and its three-dimensional parameters at 600 nm and 800 nm. A prototype system was built using commercial components.
Trace-Metal Analysis of Cancerous and Non-cancerous Human Tissues<xref ref-type="fn" rid="FN2">2</xref> (1971)

To ascertain whether any significant differences in the concentration of trace metals exist between cancerous and noncancerous human tissues, 22 trace metals and 2 bulk metals were examined by means of emission spectrography. Both cancerous and corresponding noncancerous tissue samples were taken from the same individual, and the clinical and histopathologic information was obtained. The following types of cancers were studied: ductal and scirrhous carcinoma of the breast, bronchogenic carcinoma of the lung, and adenocarcinoma of the colon. The results showed that the copper, magnesium, manganese, and zinc content of cancerous breast tissue was significantly higher than that of the noncancerous tissue. In bronchogenic carcinoma of the lung, the iron content of the cancerous tissue was significantly lower and the zinc content significantly higher than contents in the noncancerous bronchial tissue. In adenocarcinoma of the colon, the concentration of tin was considerably lower in cancerous than in noncancerous tissue. Significant differences in the concentration of selective metals were noticeable between cancerous tissues. It was also apparent that these differences pertain to a specific type of cancer. This specificity indicates that all types of cancers cannot be grouped under one category; for interpretation, each type should be regarded as a separate entity.
Context-Aware Browser (2009)

We propose the Context-Aware Browser (CAB), a new approach to context-aware Web content perusal by means of mobile devices. The CAB is a Web browser running on mobile devices, exploiting artificial intelligence techniques and mixing several ingredients. The novelty of the proposed approach is threefold. First, our proposal allows a different perspective in the development of Web contents, which should be conceived in a context-dependent way. Our framework makes it possible to develop information and web applications specifically for a given environment, which will become capable to transmit context information, and ad hoc, context-based applications to whom will enter that context. Second, we exploit several informations provided by the surrounding environment and not only the location in order to carry out a more refined search of web contents and applications. Third, the CAB approach involves an innovative two stage retrieval and a filtering process, that minimizes privacy issues.
Anticonvulsant effects of calcium channel blockers in partial and generalized model epilepsies. (1987)

The calcium channel blockers D890 and verapamil reduced neuronal calcium currents in single snail neurons with intra- and extracellular applications, respectively. Epileptic discharges of single neurons in the rat's motor cortex (in vivo) were depressed in amplitude by intracellular injection of D890. Focal seizure discharges and generalized tonic-clonic seizure activity in the cerebral cortex of the rat were reduced by intracerebroventricular perfusion of verapamil. In non-epileptic rats verapamil failed to exert a depressive effect on somatosensory evoked potentials and on the waves of the spontaneous EEG.
S/390 Parallel Enterprise Server Generation 3: A balanced system and cache structure (1997)

Since initiating the information technology industry-wide transition from bipolar to CMOS technology with the first generation of S/390® processors in 1994, IBM reached another major milestone with the introduction of the third generation in September 1996. The balanced system and cache structure and the modularity of the components of Generation 3 support a wide performance range from a uniprocessor to a high-performance multiprocessing system. Because of this modularity, Generation 4 is also based on this structure.
Decrease in Neuromuscular Control About the Knee with Maturation in Female Athletes (2004)

After girls mature, they land from a jump differently than do boys, as measured kinematically and kinetically.
Animal models of human disease. Pathology and molecular biology of spontaneous neoplasms occurring in transgenic mice carrying and expressing activated cellular oncogenes. (1989)

This present review focuses on spontaneous neoplasms occurring in transgenic mice carrying and expressing activated cellular oncogenes. The historical development of transgenic mice as in vivo disease models is briefly traced, followed by a brief description of the actual technology in such systems. Additional emphasis is placed on the concept of targeting activated cellular oncogenes to specific tissues in transgenic mice. Cumulative experience with activated (Vmyc, ras, and neu (erb-B2] oncogenes in transgenic mice is considered in detail, with particular attention paid to the observed pathology, as well as to the kinetics of disease occurrence. It is concluded that transgenic mice offer the interested investigator(s) an excellent prospective, in vivo model of oncogenesis.
On-Farm Composting: A Guide to Principles, Planning & Operations (2009)

Contains a discussion of basic composting principles, compostable materials, composting systems, the use of compost and its benefits, the steps for facility planning and operation, and the regulations that govern on-farm composting.
Wavelet Compressive Sampling Signal Reconstruction Using Upside‐Down Tree Structure (2011)

This paper suggests an upside‐down tree‐based orthogonal matching pursuit (UDT‐OMP) compressive sampling signal reconstruction method in wavelet domain. An upside‐down tree for the wavelet coefficients of signal is constructed, and an improved version of orthogonal matching pursuit is presented. The proposed algorithm reconstructs compressive sampling signal by exploiting the upside‐down tree structure of the wavelet coefficients of signal besides its sparsity in wavelet basis. Compared with conventional greedy pursuit algorithms: orthogonal matching pursuit (OMP) and tree‐based orthogonal matching pursuit (TOMP), signal‐to‐noise ratio (SNR) using UDT‐OMP is significantly improved.
ON MEASURING THE PERMITTIVITY TENSOR OF AN ANISOTROPIC MATERIAL FROM THE TRANSMISSION COEFFICIENTS (2008)

The permittivity tensor of an anisotropic material can be predicted with use of the presented technique. A slab of this substance possessing infinitesimal thickness is illuminated by a normally incident plane wave and rigorous expressions for the transmission coefficients are obtained. The derived formulas are linearly expanded with respect to the small thickness of the slice, while simple approximations of the material permittivities are produced by measuring the transmission coefficients for suitable polarizations. These simplified expressions provide a physical intuition about the use and the function of the anisotropy parameters which cannot be achieved via more precise but also more complex patterns. Some diagrams of the prediction error with respect to the dielectric constants, the size of the slab and the operating frequency are included and discussed.
Humidity‐Compensating Sensor for Volatile Organic Compounds Using Stacked Porous Silicon Photonic Crystals (2008)

Abstract One‐dimensional photonic crystals constructed from multilayered stacks of porous Si are used as sensors for gas‐phase volatile organic compounds (VOCs). The ability of a double‐stack structure to provide compensation for drift due to changing relative humidity (RH) is investigated. In this approach, two separate photonic crystals (dielectric stacks) are etched into a crystalline Si substrate, one on top of the other. The top stack is chemically modified to be hydrophobic (by hydrosilylation with dodecene) and the bottom stack is made hydrophilic (by hydrosilylation with undecylenic acid). It is shown that the optical spectrum of the double‐stack structure provides an effective means to discriminate VOCs from water vapor. In this approach, shifts in the peak frequencies from both photonic crystals are measured simultaneously. Because the two stacks respond differently to water and to VOC, the effect of changing humidity can be nulled by calculating the weighted difference between the two peak frequencies. Reliable determination of the concentration of VOC vapor in nitrogen over a range of RH values (25% < RH < 75%) is demonstrated. The ability of the double‐stack structure to discriminate between water vapor and VOCs is quantified for four different VOCs: toluene, dimethyl methylphosphonate (DMMP), heptane, and ethanol.
Packaging and microcooling of high power diode laser bars (1998)

Lifetime and reliability of high power diode laser bars (HPDLBs) are sensitively related to operating temperature, mounting stress and solder electromigration. These three factors have been taken into account for the development of a new packaging technology for 1 cm laser bars of gallium arsenide. We examine the use of chemical vapour deposited (CVD) diamond as heatspreaders in order to reduce thermal resistance of a microchannel cooler for liquid cooling. We show that it's possible to perform hard soldering on CVD-diamond with a new technique. Additionally, we present a controlled water cooling system fit to the flow characteristics of the cooler. It permits to adjust the emission wavelength of the diode lasers by changing the water flux.
Dimensions of Drinking among Male College Students (1967)

Dimensions of Drinking Among Male College Students Get access Peter Park Peter Park University of Massachusetts Search for other works by this author on: Oxford Academic PubMed Google Scholar Social Problems, Volume 14, Issue 4, Spring 1967, Pages 473–482, https://doi.org/10.2307/798859 Published: 07 August 2014
Treatment of experimental autoimmune arthritis by nasal administration of a type II collagen-cholera toxoid conjugate vaccine (1999)

Nasal treatment with CTB-CII appears to result in decreased peripheral Th1 and Th2 responses to collagen. These results suggest that intranasal vaccination with CTB-CII may offer an effective immunotherapeutic means for the control of chronic polyarthritis.
Influence of ouabain on cell inactivation by irradiation. (1996)

It is concluded that ouabain is strongly dose modifying in the human cell lines HeLa and A549 at concentrations which correlate with the inhibition of the Na(+)-K(+)-ATPase. Ouabain also inhibits the recovery of cells blocked in the cell cycle by irradiation.
Asymmetrical Duty Cycle Controlled Full-Bridge LLC Resonant Converter with Equivalent Switching Frequency Doubler (2015)

In the conventional full-bridge LLC converter, the duty cycle is kept as 0.5 and the phase-shift angle of the half-bridge modules is 0° to be a symmetrical operation, which makes the resonant tank operating frequency only equal to the switching frequency of the power devices. By regulating the duty cycles of the upper and lower switches in each half-bridge module to be 0.75 and 0.25 and the phase-shift angle of the half-bridge modules to be 180°, the asymmetrical duty cycle controlled full-bridge LLC resonant converter is derived. The proposed asymmetrical duty cycle controlled scheme halves the switching frequency of the primary switches. As a result, the driving losses are effectively reduced. Compared with the conventional full-bridge LLC converter, the soft-switching condition of the derived asymmetrical controlled LLC converter becomes easier to reduce the resonant current. Consequently, the conduction losses are decreased and the conversion efficiency is improved. The asymmetrical control scheme can be also extended to the stacked structure for high input voltage applications. Finally, two LLC converter prototypes both with 200-kHz resonant frequency for asymmetrical and symmetrical control schemes are built and compared to validate the effectiveness of the proposed control strategy.
p53 Expression in Human Carcinomas: Could Flow Cytometry Be an Alternative to Immunohistochemistry? (1998)

Several studies have shown that p53 expression has important clinical implications as an indicator of prognosis and response to chemotherapy or radiotherapy in different human tumor types. Determination of p53 expression by immunohistochemistry (IHC) has been incorporated into routine practice and its reliability has been consolidated. However, flow cytometric (FCM) analysis might represent an important objective and rapid approach. In the present study we determined p53 expression by IHC and FCM on a series of 118 human solid tumors. IHC determination was performed on histological sections and FCM analysis on cell suspensions. Low correlation coefficients (rs from 0.22 to 0.57) were observed between IHC and FCM data from individual tumors. By considering the IHC approach as the gold standard, high sensitivity and low specificity were found for FCM in detecting p53 expression. The FCM analysis of p53 expression and DNA content showed p53-positive cells in all cell cycle phases. Moreover, in most breast, lung, and colon aneuploid tumors (77%), p53-positive cells were detected only in the subpopulations with abnormal DNA content. In conclusion, FCM-p53 expression cannot be used alternatively to IHC determination, and its clinical relevance remains to be validated. Nevertheless, FCM may provide important information about p53 protein expression in the different subpopulations and cell cycle phases. (J Histochem Cytochem 46:41-47, 1998)
Multinuclear nuclear magnetic resonance study of three aqueous lanthanide shift reagents: complexes with EDTA and axially symmetric macrocyclic polyamino polyacetate ligands (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultinuclear nuclear magnetic resonance study of three aqueous lanthanide shift reagents: complexes with EDTA and axially symmetric macrocyclic polyamino polyacetate ligandsCharles C. Bryden, Charles N. Reilley, and Jean F. DesreuxCite this: Anal. Chem. 1981, 53, 9, 1418–1425Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/ac00232a027https://doi.org/10.1021/ac00232a027research-articleACS PublicationsRequest reuse permissionsArticle Views411Altmetric-Citations82LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Scanning Wiener-fringe microscope with an optical fiber tip (1992)

A scanning probe optical microscope using the Wiener fringe is presented. The Wiener fringe is formed by a standing wave between the incident and reflected waves on an optically reflective surface and is detected by inserting an optical fiber tip into the fringe-field region. The detected signal is used to maintain the tip-sample distance constant so that a topographic image of a sample can be obtained by a computer-assisted instrument. A spatial resolution of 200 nm has been achieved by observing a sample of known geometry.
Misuse of corticosteroid metered-dose inhaler is associated with decreased asthma stability (2002)

This study assessed whether the improper use of pressurized metered-dose inhalers (pMDIs) is associated with decreased asthma control in asthmatics treated by inhaled corticosteroids (ICS). General practitioners (GPs) included consecutive asthmatic outpatients treated by pMDI-administered ICS and on-demand, short-acting beta2-agonists. They measured an asthma instability score (AIS) based on daytime and nocturnal symptoms, exercise-induced dyspnoea, beta2-agonist usage, emergency-care visits and global perception of asthma control within the preceding month; the inhalation technique of the patient also was assessed. GPs (n=915) included 4,078 adult asthmatics; 3,955 questionnaires were evaluable. pMDI was misused by 71% of patients, of which 47% was due to poor coordination. Asthma was less stable in pMDI misusers than in good users (AIS: 3.93 versus 2.86, p<0.001). Among misusers, asthma was less stable in poor coordinators (AIS: 4.38 versus 3.56 in good coordinators, p<0.001). To conclude, misuse of pressurized metered-dose inhalers, which is mainly due to poor coordination, is frequent and associated with poorer asthma control in inhaled corticosteroid-treated asthmatics. This study highlights the importance of evaluating inhalation technique and providing appropriate education in all patients, especially before increasing inhaled corticosteroid dosage or adding other agents. The use of devices which alleviate coordination problems should be reinforced in pressurized metered-dose inhaler misusers.