gebhart/patrepeval huggingface dataset:
Genomic origin and transcriptional regulation of two variants of cGMP‐binding cGMP‐specific phosphodiesterases (1999)

We have reported alternative splice variants of cGMP-binding cGMP-specific phosphodiesterases (PDE5A), i.e. rat PDE5A2, human PDE5A1, canine PDE5A1 and PDE5A2, which possess distinct N-terminal sequences. In this study, the DNA sequences corresponding to the unique N-terminal portions of PDE5A1 and PDE5A2 were shown to be tandemly located upstream of exons encoding the common region of PDE5A in both human and rat PDE5A genes. The presence of human PDE5A2 and rat PDE5A1 transcripts in lung was confirmed by reverse transcriptase-PCR. These results indicated that two variant forms of PDE5A exist in humans, canines and rats. We examined the tissue distribution of the two variants of human PDE5A in adult and fetal humans. The patterns of expression of the two alternatively spliced transcripts of human PDE5A in human tissues differed. Many putative regulatory elements including cAMP response elements were observed in the 5'-untranslated region and intron of the PDE5A gene. The levels of the PDE5A transcripts, especially the PDE5A2 transcripts, were increased by a cAMP analogue in cultured rat vascular smooth muscle cells, indicating that the PDE5A2 is an inducible variant of PDE5A in rats.
Regulation of <i>Escherichia coli secA</i> by Cellular Protein Secretion Proficiency Requires an Intact Gene X Signal Sequence and an Active Translocon (1998)

secA is translationally regulated by the protein secretion proficiency state of the Escherichia coli cell. This regulation was explored by making signal sequence mutations in the gene upstream of secA, gene X, which promotes secA translational coupling. Gene X signal sequence mutants were constitutive for secA expression, while prlA alleles partially restored secA regulation. These results show that interaction of the pre-gene X protein with the translocon is required for proper secA regulation. Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor.
Antioxidant properties of palm fruit extracts. (2005)

Phenolic compounds have been shown to exhibit bioactive properties, and in particular antioxidant effects. A phenolic-rich fraction has been isolated from the aqueous by-product obtained during the milling of oil palm fruits. The objectives of the study were to determine the phenolic content of the crude and ethanolic extracts of oil palm fruits and to evaluate the antioxidant properties of these extracts. The total phenolics content of the crude and ethanol extracts as determined by the Folin-Ciocalteu method were found to be 40.3 +/- 0.5 and 49.6 +/- 0.6 mg GAE/g extract (dry basis), respectively. The radical scavenging activity of the extracts determined using 2,2 diphenyl-1-picrylhydrazyl radical (DPPH.) indicated that both crude and ethanol extracts exhibit hydrogen-donating capacity, and have antiradical power (ARP) comparable to ascorbic acid. The DPPH radical scavenging activity of the extracts were less than that of gallic acid, but the time-course variations of the scavenging curves suggest that the extracts acted by a mechanism similar to that of gallic acid. The electron-donating potentials of the extracts were inferred from the hydrogen peroxide scavenging and reducing power assays. The reducing power of crude and ethanol extracts at 1 mM GAE were found to be comparable to that of 0.3 mM gallic acid. The extracts indicated complete scavenging of hydrogen peroxide at concentrations above 0.4 mM GAE. These findings suggest that the crude and ethanol extracts are able to scavenge free radicals, by either hydrogen or electron donating mechanisms, and can therefore act as primary antioxidants.
Epitaxial growth over optical gratings on GaAs (1974)

Epitaxial layers of GaAs have been successfully grown on corrugated GaAs substrates. Growth techniques were such that the corrugation was preserved during the growth process. Growth conditions are described which permit p-n junctions containing corrugations of period suitable for electrically pumped distributed-feedback lasers in GaAs to be produced. This work demonstrates that there are no fundamental limitations on growing buried corrugations in GaAs for a large variety of integrated optical devices.
What to Do about High-Frequency Trading (2013)

The author discusses his views on an issue of interest to FAJ readers.
PepTool™ and GeneTool™: Platform-Independent Tools for Biological Sequence Analysis (2003)

Although we are unable to discuss all of the functionality available in PepTool and GeneTool, it should be evident from this brief review that both packages offer a great deal in terms of functionality and ease-of-use. Furthermore, a number of useful innovations including platform-independent GUI design, networked parallelism, direct internet connectivity, database compression, and a variety of enhanced or improved algorithms should make these two programs particularly useful in the rapidly changing world of biological sequence analysis. More complete descriptions of the programs, algorithms and operation of PepTool and GeneTool are available on the BioTools web site (www.biotools.com), in the associated program user manuals and in the on-line Help pages.
Analysis of digital evidence in identity theft investigations (2010)

Identity theft could be currently considered as a significant problem in the modern internet driven era. This type of computer crime can be achieved in a number of different ways; various statistical figures suggest it is on the increase. It intimidates individual privacy and self assurance, while efforts for increased security and protection measures appear inadequate to prevent it. A forensic analysis of the digital evidence should be able to provide precise findings after the investigation of Identity Theft incidents. At present, the investigation of Internet based Identity Theft is performed on an ad hoc and unstructured basis, in relation to the digital evidence. This research work aims to construct a formalised and structured approach to digital Identity Theft investigations that would improve the current computer forensic investigative practice. The research hypothesis is to create an analytical framework to facilitate the investigation of Internet Identity Theft cases and the processing of the related digital evidence. This research work makes two key contributions to the subject: a) proposing the approach of examining different computer crimes using a process specifically based on their nature and b) to differentiate the examination procedure between the victim’s and the fraudster’s side, depending on the ownership of the digital media. The background research on the existing investigation methods supports the need of moving towards an individual framework that supports Identity Theft investigations. The presented investigation framework is designed based on the structure of the existing computer forensic frameworks. It is a flexible, conceptual tool that will assist the investigator’s work and analyse incidents related to this type of crime. The research outcome has been presented in detail, with supporting relevant material for the investigator. The intention is to offer a coherent tool that could be used by computer forensics investigators. Therefore, the research outcome will not only be evaluated from a laboratory experiment, but also strengthened and improved based on an evaluation feedback by experts from law enforcement. While personal identities are increasingly being stored and shared on digital media, the threat of personal and private information that is used fraudulently cannot be eliminated. However, when such incidents are precisely examined, then the nature of the problem can be more clearly understood.
<i>In Vitro</i> –Cultured Human Islet Cell Monolayers: Stemness Markers and Insulin Recovery upon Streptozotocin Exposure (2009)

We have investigated long-term in vitro-cultured human islet-derived cell monolayers (CM) to determine their suitability as source of newly generated beta-cells. Initial loss of the islet three-dimensional architecture resulted in rearrangement of pancreatic hormone and key transcriptional factor expression, or decreased insulin secretion, or declined glucose-stimulated insulin release, reflecting reversal to an immature precursor stage. However, upon exposure to several streptozotocin (STZ) concentrations, CM showed glucose-stimulated insulin release recovery and an increased synthesis of insulin mRNA. Possibly, this outcome could derive from eventual "stemness" properties of such cell populations, unfolded by subtoxic STZ-induced CM damage. To test this hypothesis, we have examined messenger and protein expression of embryonic stem cell markers (Nanog, Sox-2, Oct-4A), which varied in CM versus control whole human islets. Moreover, different STZ concentrations were shown to modulate marker expression in CM. All together our data preliminarily seem to indicate that CM may generate reconstituted insulin-producing cells likely due to their intrinsic, preexisting stemness properties.
Sonochemical Synthesis of Nanostructured Molybdenum Sulfide (1998)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSonochemical Synthesis of Nanostructured Molybdenum SulfideMillan M. Mdleleni, Taeghwan Hyeon, and Kenneth S. SuslickView Author Information School of Chemical Sciences University of Illinois at Urbana-Champaign 601 South Goodwin Avenue, Urbana, Illinois 61801 Cite this: J. Am. Chem. Soc. 1998, 120, 24, 6189–6190Publication Date (Web):June 5, 1998Publication History Received5 January 1998Published online5 June 1998Published inissue 1 June 1998https://pubs.acs.org/doi/10.1021/ja9800333https://doi.org/10.1021/ja9800333rapid-communicationACS PublicationsCopyright © 1998 American Chemical SocietyRequest reuse permissionsArticle Views2707Altmetric-Citations278LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Catalysts,Catalytic activity,Molybdenum,Sulfides,Thiophenes Get e-Alerts
Emerging Trends in Energy Management (2010)

Energy is the measure of the development of any nation. Booming economic growth, rapid industrialization and high standard of living of the global population demand more and more energy in different forms. Since the quantity of available energy from conventional resources is depleting day by day, development of newer or renewable energy technologies and improvement of conventional technologies become necessary to meet the energy demand in the future. The world is undergoing a period of global climate change. Growing demand for energy despite limited fossil fuel reserves and growing environmental concerns due to increased emissions of carbon dioxide and methane, well-known green house gases, is undoubtedly the major challenge of the 21st century. It is of international importance that technological solutions can be brought to bear to solve these problems as well as providing alternative sources of power and energy. To achieve a sustainable development, the origin and the use of energy have to be addressed, and advanced energy technologies for both fossil and renewable energy carriers have to be developed. There is need to think globally but act locally. The adoption of new energy sources, energy carriers and better energy management will not only affectnthe energy market but will also have social economic and environmental impacts. New energy technologies and particular heating and cooling technologies are decentralized and will create markets and employment essentially at a local level and, therefore, will induce a modification of individual behavior. From an economic viewpoint, new energy technologies are capital intensive sources of energy and the present period is particularly interested in the development of such technologies and systems due to relatively low cost of money and high cost of energy. The scientific community has not only to think and develop advanced energy technologies but also to contribute in improving the existing ones. Even if renewable energies and new energy carriers, such as hydrogen, are promising solutions, our society still relies on fossil fuels as primary energy for many applications.
Rapid and simple method for screening of natural antioxidants from Chinese herb Flos Lonicerae Japonicae by DPPH‐HPLC‐DAD‐TOF/MS (2008)

A rapid and simple method has been developed for the screening and identification of natural antioxidants of Flos Lonicerae Japonicae (FLJ), derived from the flower buds of Lonicera japonica. The hypothesis is that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas (PAs) of compounds with potential antioxidant effects in the HPLC chromatograms will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS hyphenated technique. Using the proposed approach, about 14 compounds in the FLJ extract were found to possess a potential antioxidant activity. They were identified as chlorogenic acid (1), 1-O-caffeoylquinic acid (1-O-CQA, 2), caffeic acid (4), 4-O-CQA (5), rutin (7), isoquercitrin (8), luteolin-7-O-glucoside (9), lonicerin (10), 4,5-O-dicaffeoylquinic acid (4,5-O-diCQA, 11), 3,5-O-diCQA (12), 1,3-O-diCQA (13), 3,4-O-diCQA (14), 1,4-O-diCQA (16), and luteolin (17). In addition, the free radical scavenging capacities of the available identified compounds were also investigated by HPLC assay. The results indicated that the compounds with PAs significantly decreasing were natural antioxidants, whereas those with PAs not changing presented no activities, which accordingly indicated that this newly proposed method could be widely applied for rapid screening and identification of natural antioxidants from complex matrices including Chinese herbal medicines.
Viable Embryos from Injection of Round Spermatids into Oocytes (1995)

To the Editor: By injecting sperm into the cytoplasm of an oocyte1 it is now possible to achieve fertilization and pregnancy in cases of obstructive azoospermia or severe oligozoospermia and asthen...
A novel complex locus UGT1 encodes human bilirubin, phenol, and other UDP-glucuronosyltransferase isozymes with identical carboxyl termini. (1992)

Two human liver UDP-glucuronosyltransferase (transferase) cDNAs, HUG-Br1 and HUG-Br2, were previously isolated (Ritter, J. K., Crawford, J. M., and Owens, I. S. (1991) J. Biol. Chem. 266, 1043-1047), and each was shown to encode a bilirubin transferase isozyme which catalyzes the formation of all physiological conjugates of bilirubin IX alpha following expression in COS-1 cells. Sequence data showed that the cDNAs contained identical 3' ends (1469 base pairs in length) to each other and to that of the human phenol transferase cDNA, HLUG P1 (Harding, D., Fournel-Gigleux, S., Jackson, M. R., and Burchell, B. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8381-8385). Here we report that the two corresponding bilirubin transferases and the phenol transferase are encoded by a novel locus, UGT1, which is also predicted to encode three other bilirubin transferase-like isozymes all having identical carboxyl termini. The transcriptional arrangement utilizes six nested promoter elements, each of which is positioned upstream of a unique exon 1. Each exon 1 encodes the NH2-terminal domain (286 amino acids) and confers the substrate specificity of the isoform. The 3' end of the locus contains 4 common exons which encode the identical carboxyl termini (246 amino acids). It is predicted that six nested primary transcripts are synthesized and that each exon 1 is differentially spliced to the 4 common exons to produce six unique, mature mRNAs. Although the gene organization is present as a single copy, it provides the flexibility of independent regulation of each isoform which is known to occur in the case of bilirubin and phenol transferase activities. With an understanding of the gene structure, lethal, as well as the nonlethal defects, associated with bilirubin transferase activity can now be determined.
Novel Digital Controller Improves Dynamic Response and Simplifies Design Process of Voltage Regulator Module (2007)

A novel digital controller is presented in this paper that significantly improves the dynamic response of a voltage regulator module (VRM). The controller uses a digital linear scheme during steady-state operation and an innovative non-linear scheme during transient load conditions. The proposed non-linear controller accurately calculates the optimal response to an arbitrary load step variation. This paper demonstrates the design procedure of a VRM using the proposed controller. Since the response to the load variation is predictable, an engineer is able to design (without iteration) a VRM that is guaranteed to meet a set of dynamic response criteria (voltage deviation, recovery time).
Transmission properties of composite metamaterials in free space (2002)

We propose and demonstrate a type of composite metamaterial which is constructed by combining thin copper wires and split ring resonators (SRRs) on the same board. The transmission measurements performed in free space exhibit a passband within the stop bands of SRRs and thin wire structures. The experimental results are in good agreement with the predictions of the transfer matrix method simulations.
Polyphenols as dietary supplements: A double-edged sword (2009)

Polyphenols as dietary supplements: A double-edged sword Keith R Martin, Christy L AppelNutrition Program, Healthy Lifestyles Research Center, College of Nursing and Health Innovation, Arizona State University, Mesa, AZ, USAAbstract: Increased consumption of fruits and vegetables is associated with a lower risk of chronic disease such as cardiovascular disease, some forms of cancer, and neurodegeneration. Pro-oxidant-induced oxidative stress contributes to the pathogenesis of numerous chronic diseases and, as such, dietary antioxidants can quench and/or retard such processes. Dietary polyphenols, ie, phenolic acids and flavonoids, are a primary source of antioxidants for humans and are derived from plants including fruits, vegetables, spices, and herbs. Based on compelling evidence regarding the health effects of polyphenol-rich foods, new dietary supplements and polyphenol-rich foods are being developed for public use. Consumption of such products can increase dietary polyphenol intake and subsequently plasma concentrations beyond expected levels associated with dietary consumption and potentially confer additional health benefits. Furthermore, bioavailability can be modified to further increase absorption and ultimately plasma concentrations of polyphenols. However, the upper limit for plasma concentrations of polyphenols before the elaboration of adverse effects is unknown for many polyphenols. Moreover, a considerable amount of evidence is accumulating which supports the hypothesis that high-dose polyphenols can mechanistically cause adverse effects through pro-oxidative action. Thus, polyphenol-rich dietary supplements can potentially confer additional benefits but high-doses may elicit toxicity thereby establishing a double-edge sword in supplement use.Keywords: antioxidant, bioavailability, flavonoids, polyphenols, supplement
Limitations of Current Prophylaxis Against Influenza Virus Infection (2007)

Avian influenza has been a source of worldwide concern since Hong Kong authorities detected the first outbreak in 1997. Mainly as a result of poultry-to-human transmission, more than 200 cases of infection in humans have been attributed to the A/H5, A/H7, and A/H9 viral subtypes, with a case fatality rate for A/H5N1 infections exceeding 50%. A mutant or reassortant virus capable of efficient human-to-human transmission can set off a pandemic. Increased attention to prophylaxis against viral infection has identified several potentially complementary approaches: nonpharmacologic measures (eg, travel restrictions), vaccination, chemotherapeutic agents, and herbal/natural products. All have significant limitations that point out the need for additional modalities. Herbal/natural products, particularly those based on green tea extract, offer promise as adjuncts or alternatives to current interventions and warrant further evaluation in well-controlled human trials.
Pilot correlation positioning method for urban UMTS networks (2005)

Abstract – The aim of this paper is to present a novel mobile positioning method for urban deployment of UMTS network. Proposed technique is entirely network-based, thus not involving any modifications in the terminals. The method presented in this paper utilizes information accessible in the SRNC describing the visible pilot RSCP measurements of a particular UE. Least square method is used for selecting the most similar situation from the set of pre-measured pilots strength samples stored in location server in the network. The performance of the method has been examined by intensive measurement campaigns carried out in Elisa UMTS network in Tampere, Finland. Field trials have provided very promising results, since on the average, the position of the user can be estimated with the accuracy at the level of 190 m for 90 % of measurements and 90 m with 67% CERP. 1.
On-line structural health and fire monitoring of a composite personal aircraft using an FBG sensing system (2008)

We report in this paper on the design and development of a novel on-line structural health monitoring and fire detection system based on an array of optical fiber Bragg grating (FBG) sensors and interrogation system installed on a new, precommercial compact aircraft. A combined total of 17 FBG sensors - strain, temperature and high-temperature - were installed at critical locations in an around the wings, fuselage and engine compartment of a prototype, Comp Air CA 12 all-composite, ten-passenger personal airplane powered by a 1,650 hp turbine engine. The sensors are interrogated online and in real time by a swept laser FBG interrogator (Micron Optics sm125-700) mounted on board the plane. Sensors readings are then combined with the plane's avionics system and displayed on the pilot's aviation control panel. This system represents the first of its kind in commercial, small frame, airplanes and a first for optical fiber sensors.