gebhart/patrepeval huggingface dataset:
Plasma separation using a hollow fiber membrane device (1986)

A disposable hollow fiber device was evaluated by collecting approximately 550 ml of normal donor plasma (n = 43) and by performing sham (n = 10) and therapeutic (n = 12) plasma exchanges. Blood was processed at 70 ml per min, and plasma flux averaged 23 (collection) and 25 (exchange) ml per min (mean separation efficiencies of 52 and 60%, respectively). The procedures were tolerated well by all donors and patients. The plasma hemoglobin concentration in separated plasma averaged 1 mg per dl, and cell contamination was negligible (mean of 1, 3, and 6 RBCs, platelets and WBCs/microliter, respectively). There was no evidence of in vivo classical or alternative pathway complement activation as assessed by total hemolytic complement generation (CH50), alternative pathway hemolytic activity (AP50), C3 conversion, or C5 activation, nor were unexpected changes seen in the results of laboratory tests performed after the procedure. Sieving coefficients during sham plasma exchange averaged as follows: albumin, 1.03; IgM, 1.0, IgG, 1.0; IgA, 0.98; factor V, 1.07; factor VII, 0.89; factor VIII, 1.05; and factor IX, 1.19. The device appears to be useful for separation of cell-free plasma from blood during therapeutic plasma exchange procedures.
EFFECTS OF VITAMIN B<sub>12</sub> ON SERUM LIPIDS AND LIPOPROTEINS OF PIGS FED DIETS SUPPLEMENTED WITH PROPIONIC ACID OR CALCIUM PROPIONATE (1982)

Effects of vitamin B 12 supplementation of diets containing propionic acid (PA), or calcium propionate (CP) on feed intake, serum lipids and lipoproteins were studied utilizing 80 crossbred pigs averaging 22.5 kg. Diets, based on barley-soybean meal, containing 0, 3.5 or 7% PA or CP, were fed with and without the addition of 4.95 mg vitamin B 12 /kg diet. Inclusion of PA or CP with or without the addition of vitamin B 12 had no significant effect on feed intake. Addition of PA or CP depressed serum cholesterol, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol and serum triglycerides. Vitamin B 12 supplementation had no effect on serum total cholesterol or HDL cholesterol, caused a reduction in LDL cholesterol, and tended to overcome the reduction in serum triglyceride. Supplementation of diets with vitamin B 12 reduced liver lipid levels, largely as a result of a decrease in cholesterol and phospholipids. These data suggest that the cholesterol-lowering effect of PA is not simply a result of a depression in feed intake, but arises from a direct action of PA on cholesterol metabolism. Key words: Vitamin B 12 , lipids, lipo protein, pigs, propionic, propionate
Bio-inspired self-organization for supporting dynamic reconfiguration of modular agents (2009)

This paper presents an approach to develop and manage a self-organizing distributed components based system in dynamically changing environments. In the proposed approach, an application is composed of a federation of distributed, autonomous and diverse mobile agents. Each mobile agent is implemented as dynamically associated bio-inspired modular components, that can migrate and reconfigure by themselves while the application is being executed. Association between modular components can be changed and transformed according to agents' local migration schemes including deployment based on biological processes. This paper presents a an adaptive architecture, which can reorganize and reconfigure agents after several biological concepts and mechanisms. We describe several key features of the modular components, depict the design and implementation methodologies of the modular component-based self-organizable framework, and demonstrate how the framework satisfies a set of functional requirements derived from the features of our agents. We also present some simulation results to examine scalability and efficiency of the framework.
Colorectal Cancer Mortality: Effectiveness of Biennial Screening for Fecal Occult Blood (1999)

The results from this study, together with the other two published randomized trials of fecal occult blood screening, are consistent in demonstrating a substantial, statistically significant reduction in colorectal cancer mortality from biennial screening.
PHYSIOLOGIC CURVE OF RESPONSE TO PHYTOHORMONES BY SEEDS, GROWING PLANTS, CUTTINGS, AND LOWER PLANT FORMS (1937)

In all plant species tested, increasing concentrations of phytohormones produced responses falling on a physiologic curve from minimum through optimum to maximum which, if exceeded, led to injury and death. Indolyl-acetic acid, its butyric and propionic homologues, naphthylacetic acid, their salts, and mixtures gave similar results. Treating seeds with hormones incorporated in adsorbent dust stimulated both root and top growth markedly, with less danger of overdosage than in solution treatment. Dosages equivalent to 50 to 250 mg. per acre applied as dilute solutions to soil growing young lettuce and tomato plants covered the optimum range of stimulation to growth. Dust treatment of cuttings proved very convenient and successful in inducing rooting, the plants again showing a wider range of tolerance to dusts than to solutions. Fermentation of sugar by yeast responded to hormone stimulation. Various practical applications are discussed.
Investigation of the Immunogenicity of a Protein Drug Using Equilibrium Dialysis and Liquid Chromatography Tandem Mass Spectrometry Detection (2005)

Biotherapeutics such as protein and peptide drugs have attracted significant attention in the medical community and pharmaceutical industry in recent years. Immunogenicity is one of the major concerns in the development and application of biotherapeutics. Although great efforts have been put forth in reducing immunogenicity, monitoring the free ("active") drug concentration and the antibody formation is critical for preclinical and clinical studies. Currently, it is still a challenging task to have a standardized test method monitoring immunogenicity when biotherapeutic compounds such as proteins and peptides are administrated. Combined with liquid chromatography/tandem mass spectrometry detection, the equilibrium dialysis technique that is conventionally used for measuring the free and bound concentration of small organic molecules was extended to the application of measuring the free and bound concentrations of a protein drug with a relative molecular mass over 10,000 from plasma samples containing antibody. This novel approach could also be used for accurately measuring the antibody concentration when a reference standard of the antibody is available.
Vigabatrin, but not Gabapentin or Topiramate, Produces Concentration‐related Effects on Enzymes and Intermediates of the GABA Shunt in Rat Brain and Retina (2003)

These findings corroborate a previously reported accumulation of VGB in the retina, which may be responsible for the visual field constriction observed clinically. This phenomenon does not appear to extend to other GABAergic drugs, suggesting that these agents might not cause visual field defects.
Palladium-catalyzed decarboxylation-carbonylation of allylic carbonates to form .beta.,.gamma.-unsaturated esters (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPalladium-catalyzed decarboxylation-carbonylation of allylic carbonates to form .beta.,.gamma.-unsaturated estersJiro Tsuji, Koji Sato, and Hiroshi OkumotoCite this: J. Org. Chem. 1984, 49, 8, 1341–1344Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/jo00182a005https://doi.org/10.1021/jo00182a005research-articleACS PublicationsRequest reuse permissionsArticle Views1969Altmetric-Citations142LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Realism vs. optimality: a systems-engineered wide area network design system (2002)

Research is described in modeling, analysis and design of large scale, wide area communications networks. The thrust of the work has been directed towards large scale packet-switched data communications networks employing the US Department of Defense (DoD) suite of protocols. While there are a number of peculiarities (e.g., objectives and constraints on designs) specific to DoD technology networks which may not be found in all non-DoD environments, the DoD environment embodies many of the complicating aspects of a variety of data communications technologies: uncertain traffic; dynamic, adaptive routing; multiple, conflicting, and noncommensurate objectives; a broad spectrum of network sizes and purposes; numerous network reliability and survivability concerns; and internetworking.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Exact mass measurement by Fourier transform mass spectrometry (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExact mass measurement by Fourier transform mass spectrometryE. B. Ledford, Sahba. Ghaderi, R. L. White, R. B. Spencer, P. S. Kulkarni, C. L. Wilkins, and M. L. GrossCite this: Anal. Chem. 1980, 52, 3, 463–468Publication Date (Print):March 1, 1980Publication History Published online1 May 2002Published inissue 1 March 1980https://pubs.acs.org/doi/10.1021/ac50053a021https://doi.org/10.1021/ac50053a021research-articleACS PublicationsRequest reuse permissionsArticle Views240Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
G-quadruplex recognition by bis-indole carboxamides (2008)

Herein we report the de novo design and synthesis of a geometrically flexible bis-indole carboxamide and a constrained derivative, as a novel class of small molecule scaffold that exhibits high stabilization potential for DNA G-quadruplex sequences associated with the promoters of c-kit2 and c-myc.
Organization and transcription of the myo-inositol operon, iol, of Bacillus subtilis (1997)

Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analysis suggested that an inositol-inducible promoter for the iol operon (iol promoter) might be a promoter-like sequence in the 5' region of iolA, which is probably recognized by sigmaA. S1 nuclease analysis implied that a rho-independent terminator-like structure in the 3' region of iolJ might be a terminator for iol transcription. Disruption of the iol promoter prevented synthesis of the iol transcript as well as that of Idh, implying that the iol operon is most probably transcribed as an 11.5-kb mRNA containing the 10 iol genes. Immediately upstream of the iol operon, two genes (iolR and iolS) with divergent orientations to the iol operon were found. Disruption of iolR (but not iolS) caused constitutive synthesis of the iol transcript and Idh, indicating that the iolR gene encodes a transcription-negative regulator (presumably a repressor) for the iol operon. Northern and S1 nuclease analyses revealed that the iolRS genes were cotranscribed from another inositol-inducible promoter, which is probably recognized by sigmaA. The promoter assignments of the iol and iolRS operons were confirmed in vivo with a lacZ fusion integrated into the amyE locus.
Iterative Cycles of In Vitro Protein Selection for DNA Polymerase Activity (2003)

Isolation of genes encoding catalysts for defined chemical reactions should be facilitated by selection of proteins for catalysis from molecular repertoires. Display of proteins on phage allows the coupling between a protein and its gene. Furthermore, if the reaction product can be linked to the phage enzyme catalyzing the reaction, affinity chromatography for the product yields the protein catalyzing the reaction and its gene. One main advantage of this selection method is that it can be in principle generalized to most chemical reactions.Here, iterative in vitro selections for polymerase activity were used to isolate a single phage-polymerase among more than 108 phage particles. Three to five selection cycles were required depending on the fraction of infectious phage-polymerases in the initial phage population. This is the first report quantifying the enrichment over iterative selection rounds for the isolation of rare catalysts displayed on phage.
Software Installation on a Huge Heterogeneous Network using Mobile Agents (2007)

In this paper we describe a system that performs software installation on a vast heterogeneous network using mobile agents. More specifically, the Java aglets platform has been used to achieve this purpose. We require that a one time installation of the Java aglet server is done on all nodes in the network. When new software is to be installed, the central server is responsible to send agents over the network that performs the installation. Error handling and security features have also been addressed in our system.
A chimeric antibody with the human ?1 constant region as a putative standard for assays to detect IgG ?2-glycoprotein I-dependent anticardiolipin and anti-?2-glycoprotein I antibodies (1999)

Because the reactivity of HCAL is similar to that of aCL in sera from humans with APS, HCAL will be useful as a standard for human IgG aCL and anti-beta2GPI antibody assays.
Mitochondria Targeting by Guanidine− and Biguanidine−Porphyrin Photosensitizers (2008)

We report the syntheses of three new amphiphilic porphyrin derivatives, containing a guanidine, a biguanidine, or an MLS peptide, that were designed to target the cell mitochondria. The guanidine- and biguanidine-porphyrins are poorly soluble in water, forming J-type aggregates in aqueous solutions. On the other hand, the porphyrin-MLS peptide conjugate bearing a low molecular weight PEG spacer is highly water-soluble and does not aggregate in aqueous media. The fluorescence quantum yields determined for all porphyrins were higher at low pH (<6) and the porphyrin-peptide conjugate had the highest quantum yields in aqueous media. All porphyrins showed low dark toxicity toward human carcinoma HEp2 cells, and the guanidine-porphyrin was the most phototoxic (IC 50 = 4.8 microM at 1 J cm (-2)), followed by the biguanidine-porphyrin and the porphyrin-MLS (IC50 = 8.2 microM and 9.8 microM at 1 J cm (-2), respectively). The porphyrin-MLS peptide conjugate accumulated the most within cells of all porphyrins at all times investigated and the biguanidine-porphyrin accumulated the least. Both the guanidine- and biguanidine-porphyrins localized within cell mitochondria and, in addition, were found in the lysosomes and the ER (in the case of the guanidine-porphyrin). In contrast, the porphyrin-MLS peptide conjugate localized mainly within the cell lysosomes.
Exploiting shift invariance to obtain a high payload in digital image watermarking (2003)

Robust, invisible watermarking of digital images or video very often has to satisfy a set of mutually conflicting requirements. These requirements include invisibility, robustness to image processing and geometric transformations, low false positive probabilities, high payload, fast detection and embedding, etc. In this paper we show how invariance to translations can be exploited to increase the payload. This is achieved by simultaneous embedding of several shifted watermark patterns, such that the information content is hidden in the relative shifts of the patterns. The principles of this are illustrated for the case of JAWS, a spatial domain watermark method developed by Philips. The method can easily be applied to other watermark methods which are able to detect shifted versions of watermarks.
Red Rice (<i>Oryza sativa</i>) Biology. I. Characterization of Red Rice Ecotypes (1999)

Plant characteristics of red rice ecotypes obtained from Arkansas, Louisiana, Mississippi, and Texas, including 11 strawhulled, five blackhulled, two goldhulled, and one brownhulled type, were evaluated under field conditions. Most ecotypes were uniform and stable but manifested considerable genetic variability. Red rice plants had pubescent leaves, were taller with lighter green color, and produced more tillers and panicles per plant than rice cultivars ‘Lemont,’ ‘Mars,’ and ‘Maybelle.’ Most ecotypes were highly susceptible to seed shattering starting about 14 d after anthesis when seed moisture was more than 25%. Seeds of most ecotypes were highly dormant at harvest. Rice cultivars had a larger flag leaf and more total leaf area per plant at anthesis and produced more seeds per panicle than red rice. Some red rice ecotypes had plant characteristics closely related to cultivated rice suggesting natural hybridization with rice.
Between-assay variability of faecal calprotectin enzyme-linked immunosorbent assay kits (2012)

Laboratories should be aware of the lack of the assay standardization, as demonstrated by the between-assay variability. A comparison between f-Cp concentrations reported by these assays and clinical markers of disease severity is required in order to determine their diagnostic accuracy. The EQA scheme represents the first available programme for f-Cp.
The neurotrophin receptor, gp75, forms a complex with the receptor tyrosine kinase TrkA. (1996)

The high-affinity NGF receptor is thought to be a complex of two receptors , gp75 and the tyrosine kinase TrkA, but direct biochemical evidence for such an association had been lacking. In this report, we demonstrate the existence of such a gp75-TrkA complex by a copatching technique. Gp75 on the surface of intact cells is patched with an anti-gp75 antibody and fluorescent secondary antibody, the cells are then fixed to prevent further antibody-induced redistributions, and the distribution of TrkA is probed with and anti-TrkA antibody and fluorescent secondary antibody. We utilize a baculovirus-insect cell expression of wild-type and mutated NGF receptors. TrkA and gp75 copatch in both the absence and presence of NGF. The association is specific, since gp75 does not copatch with other tyrosine kinase receptors, including TrkB, platelet-derived growth factor receptor-beta, and Torso (Tor). To determine which domains of TrkA are required for copatching, we used a series of TrkA-Tor chimeric receptors and show that the extracellular domain of TrkA is sufficient for copatching with gp75. A chimeric receptor with TrkA transmembrane and intracellular domains show partial copatching with gp75. Deletion of the intracellular domain of gp75 decreases but does not eliminate copatching. A point mutation which inactivates the TrkA kinase has no effect on copatching, indicating that this enzymatic activity is not required for association with gp75. Hence, although interactions between the gp75 and TrkA extracellular domains are sufficient for complex formation, interactions involving other receptor domains also play a role.
Anodic Evolution of Oxygen on Sputtered Iridium Oxide Films (1981)

We report on the electrocatalytic properties of sputtered iridium oxide films (SIROF's) in acidic electrolytes. Long term stability (240 hr) is demonstrated. Typical steady‐state currents are 75 mA/cm2 at 1.85V (vs. RHE), which is 50% higher than previously reported. In addition, we introduce a new method of determining Tafel slopes based on the short‐time decay of the oxygen evolution reaction (OER) current, corrected for the capacitative component. Tafel plots are presented for samples of different thickness and degree of hydration. The high current density and absence of corrosion demonstrate the superior catalytic properties of SIROF's vs. iridium over the entire voltage range investigated.
Surface Potential Mapping of SAM‐Functionalized Organic Semiconductors by Kelvin Probe Force Microscopy (2010)

Kelvin probe force microscopy measurements on rubrene single crystals partially coated with fluorinated and non-fluorinated SAM derivatives are employed to determine the SAM-induced surface potentials caused by an interfacial charge-transfer doping process resulting in an interface dipole. The surface potential and topographic information in turn allow calculation of the effective intramolecular electric fields and carrier densities due to doping in the SAM-modified rubrene crystals. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
A Likelihood-Based Approach to the Estimation of Relative DNA Copy Number by Comparative Genomic Hybridization (1997)

A general approach is described for efficiently and objectively analyzing comparative genomic hybridization (CGH) profile data based on the use of realistic statistical models and the application of standard likelihood-based inference. In contrast to other methods in current use, the approach provides a most parsimonious explanation by identifying the smallest number of relative DNA copy number changes consistent with the data, together with estimates of their levels and positions and of their standard errors. By making efficient use of available data, it has the potential to enhance the resolution of CGH technology. The computational feasibility of the method is illustrated by application to real CGH profile data from human chromosome 4.