gebhart/patrepeval huggingface dataset:
Ectopic <i>Hoxa-1</i> induces rhombomere transformation in mouse hindbrain (1994)

Homeobox genes are expressed with a specific spatial and temporal order, which is essential for pattern formation during the early development of both invertebrates and vertebrates. Here we show that widespread ectopic expression of the Hoxa-1 (Hox 1.6) gene directed by a human beta-actin promoter in transgenic mice is embryolethal and produces abnormal phenotypes in a subset of domains primarily located in anterior regions. Interestingly, this abnormal development in the Hoxa-1 transgenic mice is associated with ectopic expression of the Hoxb-1 (Hox 2.9) gene in select hindbrain regions. At gestation day 9.5, two domains of strong Hoxb-1 expression are found in the anterior region of the hindbrains of Hoxa-1 transgenic embryos. One region represents the normal pattern of Hoxb-1 expression in rhombomere 4 and its associated migrating neural crest cells, while another major domain of Hoxb-1 expression consistently appears in rhombomere 2. Similar ectopic domains of beta-galactosidase activity are detected in dual transgenic embryos containing both beta-actin/Hoxa-1 transgene and a Hoxb-1/lacZ reporter construct. Expression of another lacZ reporter gene that directs beta-galactosidase activity predominantly in rhombomere 2 is suppressed in the Hoxa-1 transgenic embryos. We have also detected weaker and variable ectopic Hoxb-1 expression in rhombomeres 1, 3 and 6. No ectopic Hoxb-1 expression is detected in rhombomere 5 and the expression of Hoxa-3 and Krox-20 in this region is unchanged in the Hoxa-1 transgenic embryos. While no obvious change in the morphology of the trigeminal or facial-acoustic ganglia is evident, phenotypic changes do occur in neurons that emanate from rhombomeres 2 and 3 in the Hoxa-1 transgenic embryos. Additionally, alterations in the pattern of Hoxa-2 and Hoxb-1 expression in a subpopulation of neural crest cells migrating from the rhombomere 2 region are detected in these transgenics. Taken together, these data suggest that ectopic Hoxa-1 expression can reorganize select regions of the developing hindbrain by inducing partial transformations of several rhombomeres into a rhombomere-4-like identity.
Kinins as mediators of intestinal secretion (1989)

Kinins are small peptides that have diverse biological actions. Concentrations of kinins in the nanomolar or subnanomolar range induce intestinal smooth muscle contraction and evoke mucosal electrolyte secretion. Hyperkininemia is associated with effects on gastrointestinal motility and intestinal mucosal inflammation. Bradykinin and kallidin are the predominant kinins with effects on the gastrointestinal tract of mammals. Bradykinin stimulates chloride ion secretion by the guinea pig and rabbit ileum, rabbit colon, rat colon and monolayers of human HCA-7 cells. Kinins directly or indirectly stimulate phospholipase A2 and phospholipase C. Cells in the lamina propria of the mucosa (e.g., fibroblasts, mast cells, leukocytes), by liberating cyclooxygenase and lipoxygenase metabolites of arachidonic acid, are involved in the kinin response; direct effects on epithelial cells cannot be ruled out, however. Antagonists now exist for kinin receptors. Based on studies with these antagonists in smooth muscle preparations, two subgroups of kinin receptor have been identified. The B2-type receptor appears to be responsible for both the contraction of ileal muscle and ileal secretion. Kinins are probably more important as pathophysiological rather than as physiological mediators. They may amplify the effect of inflammatory products that induce intestinal secretion. The precise involvement of kinins in clinical mucosal secretory states and diarrhea will require quantitative assessment of their levels during each phase of mucosal inflammation. Additional studies on the mechanism of action of kinins will be essential in designing therapy to mitigate the symptoms associated with mucosal inflammation.
Protective clothing systems and materials (1994)

Protective Clothing: An Overview * Design of Protective Clothing * Chemical Protective Clothing * Attitudes and Clothing Practices of Pesticide Applicators * Refurbishing Pesticide-Contaminated Clothing * Heat Transfer Through Human Body and Clothing Systems * Protective Clothing: Use of Flame-Retardant Textile Finishes * Heat- and Fire-Resistant Fibers for Protective Clothing * Biological Protective Clothing * Protective Clothing Effective Against Biohazards * Biological Test Methods for Protective Clothing.
EFFECTS OF GLUCOCORTICOIDS ON THE RODENT HEPATOCELLULAR FRUCTOSE-1, 6-DIPHOSPHATASE ACTIVITY-LIGHT AND ELECTRON HISTOCHEMICAL STUDIES (1969)

Effects of glucocorticoids (triamcinolone diacetate (TAG) and dexamethasone) on the hepatocellular fructose-1, 6-diphosphatase (FDPase) activity was investigated histochemically in some rodents.It was observed that glucocorticoids induced the hepatocellular FDPase and this induction was heterogenous in nature, indicating the functional heterogeneity among hepatic cells. This phenomenon was named as “TAC effect”. The TAG effect is not only enzyme specific, but also species specific.
Integer multiplication and division on the HP Precision Architecture (1988)

In recent years, many architectural design efforts have focused on maximizing performance for frequently executed, simple instructions. The authors describe how a small set of primitive instructions combined with what is considered careful frequency analysis and clever programming allows the Hewlett-Packard (HP) Precision Architecture integer multiplication and division implementation to provide adequate performance at little or no hardware cost.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Language design and implementation using ruby and the interpreter pattern (2008)

In this paper, the S-expression Interpreter Framework (SIF) is presented as a tool for teaching language design and implementation. The SIF is based on the interpreter design pattern and is written in the Ruby programming language. Its core is quite small, but it can be easily extended by adding primitive procedures and special forms. The SIF can be used to demonstrate advanced language concepts (variable scopes, continuations, etc.) as well as different programming styles (functional, imperative, and object oriented).
Transradial access in an occluded radial artery: new technique. (2007)

Radial access is considered clearly impossible in patients who develop radial artery occlusion after a transradial procedure. Radial artery occlusion also prohibits an ipsilateral transulnar approach. For patient subsets such as those with dialysis access in the other upper extremity or an occluded left radial artery with left internal mammary artery bypass grafts, switching to the transfemoral approach becomes inevitable. In some instances, the femoral access is also not an option due to severe aortoiliac disease. We describe a technique that allows the operator to access the proximal circulation from the occluded radial artery.
[Determination of amylose content in transgenic progenies containing an antisense waxy gene and identifying of pure lines in indica rice]. (2004)

Through genetic transformation mediated by Agrobacterium tumefaciens, an antisense waxy gene was introduced into Longtefu B line, a male sterile maintainer line in indica (Oryza sativa L.). Thirty transgenic plants showed integration of antisense waxy gene into the genome as determined by PCR assay, and twenty eight were confirmed by Southern blotting. T1 seeds from twenty one transgenic plants showed a marked decrease of amylose content, ranging from 3%-13% less than control, and seeds from some transgenic lines exhibited typical waxy phenotype. Six transgenic lines were selected to examine the amylose content in different generations. In the T4 generation, two homozygous lines, Long 3-1-1-1 and Long 5-8-2-1, were selected, with amylose content of 15.9% and 8.4% respectively. The amylose content in these lines is in consistent with the decrease of the accumulation of Wx protein as determined by SDS-PAGE electrophoresis. Cross and subsequent backcross of Long 3-1-1-1 and Long 5-8-2-1 with the Longtefu mare sterile line were performed to determine the changes of amylose content in F1 and B1F1 seeds. The results showed an average amlyose content of 21.4% in F1 seeds with Long 3-1-1-1 as a parent, while only 13.6% with Long 5-8-2-1 as a parent. In addition, the average amylose contents in B1F1 seeds were 17.1% and 9.3% respectively. Our results indicated that during the fertility transfer in the male sterile line, stable transgenic lines with medium or low contents of amylose had direct effects on amylose content in F1 seeds.
Improved polyhydroxybutyrate production by <i>Saccharomyces cerevisiae</i> through the use of the phosphoketolase pathway (2013)

The metabolic pathways of the central carbon metabolism in Saccharomyces cerevisiae are well studied and consequently S. cerevisiae has been widely evaluated as a cell factory for many industrial biological products. In this study, we investigated the effect of engineering the supply of precursor, acetyl-CoA, and cofactor, NADPH, on the biosynthesis of the bacterial biopolymer polyhydroxybutyrate (PHB), in S. cerevisiae. Supply of acetyl-CoA was engineered by over-expression of genes from the ethanol degradation pathway or by heterologous expression of the phophoketolase pathway from Aspergillus nidulans. Both strategies improved the production of PHB. Integration of gapN encoding NADP(+) -dependent glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans into the genome enabled an increased supply of NADPH resulting in a decrease in glycerol production and increased production of PHB. The strategy that resulted in the highest PHB production after 100 h was with a strain harboring the phosphoketolase pathway to supply acetyl-CoA without the need of increased NADPH production by gapN integration. The results from this study imply that during the exponential growth on glucose, the biosynthesis of PHB in S. cerevisiae is likely to be limited by the supply of NADPH whereas supply of acetyl-CoA as precursor plays a more important role in the improvement of PHB production during growth on ethanol.
An agent-based infrastructure for energy profile capture and management (2011)

Accurately monitoring changing energy usage patterns in households is a first requirement for more efficient and eco-friendly energy management. Such data is essential to the establishment of the Smart Grid, but at this stage, domestic data collection devices are still in development and monitoring-enabled domestic appliances are rare, so that any experimental software framework must be flexible and adaptable both in respect of sensor sources and developer and user requirements. These considerations have been the drivers behind the distributed agent-based platform this paper proposes. It provides: (i) a generic sensor interface that can be specialised for new devices as required, while insulating the rest of the platform from such changes, (ii) persistent unstructured (RDF) data storage, permitting both semantic annotation and semantic-based queries, independent of data sources, and (iii) a flexible, dynamic browser interface, that allows for remote configuration of the sensor platform and accessibility via a wide range of devices. Two small case studies show the utility of the approach.
A Computational Approach to Politeness with Application to Social Factors (2013)

We propose a computational framework for identifying linguistic aspects of politeness. Our starting point is a new corpus of requests annotated for politeness, which we use to evaluate aspects of politeness theory and to uncover new interactions between politeness markers and context. These findings guide our construction of a classifier with domain-independent lexical and syntactic features operationalizing key components of politeness theory, such as indirection, deference, impersonalization and modality. Our classifier achieves close to human performance and is effective across domains. We use our framework to study the relationship between politeness and social power, showing that polite Wikipedia editors are more likely to achieve high status through elections, but, once elevated, they become less polite. We see a similar negative correlation between politeness and power on Stack Exchange, where users at the top of the reputation scale are less polite than those at the bottom. Finally, we apply our classifier to a preliminary analysis of politeness variation by gender and community.
Induction of the epibranchial placodes (1999)

The cranial sensory ganglia, in contrast to those of the trunk, have a dual embryonic origin arising from both neurogenic placodes and neural crest. Neurogenic placodes are focal thickenings of ectoderm, found exclusively in the head of vertebrate embryos. These structures can be split into two groups based on the positions that they occupy within the embryo, dorsolateral and epibranchial. The dorsolateral placodes develop alongside the central nervous system, while the epibranchial placodes are located close to the top of the clefts between the branchial arches. Importantly, previous studies have shown that the neurogenic placodes form under the influence of the surrounding cranial tissues. In this paper, we have analysed the nature of the inductive signal underlying the formation of the epibranchial placodes. We find that epibranchial placodes do not require neural crest for their induction, but rather that it is the pharyngeal endoderm that is the source of the inductive signal. We also find that, while cranial ectoderm is competent to respond to this inductive signal, trunk ectoderm is not. We have further identified the signalling molecule Bmp7 as the mediator of this inductive interaction. This molecule is expressed in a manner consistent with it playing such a role and, when added to ectoderm explants, it will promote the formation of epibranchial neuronal cells. Moreover, the Bmp7 antagonist follstatin will block the ability of pharyngeal endoderm to induce placodal neuronal cells, demonstrating that Bmp7 is required for this inductive interaction. This work answers the long standing question regarding the induction of the epibranchial placodes, and represents the first elucidation of an inductive mechanism, and a molecular effector, underlying the formation of any primary sensory neurons in higher vertebrates.
Lower extremity free flap reconstruction outcomes using venous coupler (2011)

Microvascular anastomotic coupling devices create effective venous anastomoses in lower extremity microvascular reconstruction. Thus, it presents an important tool in the armamentarium for lower extremity microsurgical reconstruction.
Case report: reduction of low back pain in a professional golfer (2000)

Previous research agrees that the majority of injuries that affect male golfers are located in the lower back and that they are related to improper swing mechanics and/or the repetitive nature of the swing. This study describes the trunk motion and paraspinal muscle activity during the swing of a golfer with related low back pain (LBP) and assesses the effect of a 3-month period of muscle conditioning and coaching on these variables. Motion of the trunk was measured using three-dimensional video analysis and electromyograms (EMGs) were recorded from the same six sites of the erector spinae at the start and end of the 3-month period. At the end of the period, the golfer was able to play and practice without LBP. Coaching resulted in an increase in the range of hip turn and a decrease in the amount of shoulder turn, which occurred during the swing. In addition, a reduction in the amount of trunk flexion/lateral flexion during the downswing occurred in conjunction with less activity in the left erector spinae. These changes may serve to reduce the torsional and compressive loads acting on the thoracic and lumbar spine, which in turn may have contributed to the cessation of the LBP and would reduce the risk of reoccurrence in the future. In conclusion, further research with more subjects would now be warranted in order to test the findings of this program for the prevention of low back in golfers as piloted in this case report.
Injection and flow control system for microchannels (2004)

In spite of considerable efforts, flow control in micro-channels remains a challenge owing to the very small ratio of channel/supply-system volumes, as well as the induction of spurious flows by extremely small pressure or geometry changes. We present here an inexpensive and robust system for flow control in a microchannel system, based on a dynamic control of reservoir pressures at the end of each channel. This system allows flow equilibration with a time constant smaller than one second, and is also able to maintain stable flux from stopped flow to many microl min(-1) range over several hours. It is robust to changes in ambient pressure and temperature. This system further includes a feature for sub-microliter sample injection during the experiment. We quantify flow control in elastomer and thermoplastic channels, and demonstrate the impact on one application of the system, namely the reproducible, automated separation of large DNA by electrophoresis in a self-organized magnetic bead matrix in a microchannel.
Maximum Transmission Rate of PSR/TSR Protocols in Wireless Energy Harvesting DF-Based Relay Networks (2015)

In this paper, we consider the power splitting relaying (PSR) and time switching relaying (TSR) protocols for decode-and-forward (DF)-based relay networks consisting of a source, a relay, and a destination. For the networks, the relay is assumed to have a rechargeable battery with a certain amount of remaining energy for energy harvesting through the received signal transmitted from the source. Specifically, for the PSR protocol with and without the direct-path, we present the outage probability expression with a given power splitting coefficient, and we obtain the optimum power splitting coefficient to maximize the transmission rate and derive its transmission rate and outage probability, where the optimum coefficient depends on channel conditions and remaining energy. For the TSR protocol with and without the direct-path, we present the outage probability expression with a given time switching coefficient, and we obtain the optimum time switching coefficient to maximize the transmission rate and derive its transmission rate, where the optimum coefficient also depends on channel conditions and remaining energy.
On Tracing Rays Through an Optical System (1914)

Trigonometrical formulae have been used for tracing rays not lying entirely in one plane through optical systems, as these can readily be arranged in a form suitable for logarithmic calculation. When a calculating machine is available such computations can be carried out more expeditiously by using algebraic formulae given in the Paper; in form these exactly correspond with the expressions for paraxial rays, and a comparison of the numerical result appears likely to suggest what alterations should be made when a general ray does not behave as desired. It is pointed out that if the two points in which a general ray meets an axial plane are defined as conjugate points, all pairs of conjugate points on a ray are connected by the same relations as hold for object and image points for paraxial rays, and the theory for paraxial rays can be extended to rays in genera by placing a suitable interpretation on magnification, &c. The definition of conjugate points can be extended to include rays lying in axial planes, in which case the one point marks the intersection of the ray with the radial focal line formed by rays passing through its conjugate.
Rational Design, Synthesis, and Optical Properties of Film‐Forming, Near‐Infrared Absorbing, and Fluorescent Chromophores with Multidonors and Large Heterocyclic Acceptors (2009)

A new series of film-forming, low-bandgap chromophores (1 a,b and 2 a,b) were rationally designed with aid of a computational study, and then synthesized and characterized. To realize absorption and emission above the 1000 nm wavelength, the molecular design focuses on lowering the LUMO level by fusing common heterocyclic units into a large conjugated core that acts an electron acceptor and increasing the charge transfer by attaching the multiple electron-donating groups at the appropriate positions of the acceptor core. The chromophores have bandgap levels of 1.27-0.71 eV, and accordingly absorb at 746-1003 nm and emit at 1035-1290 nm in solution. By design, the relatively high molecular weight (up to 2400 g mol(-1)) and non-coplanar structure allow these near-infrared (NIR) chromophores to be readily spin-coated as uniform thin films and doped with other organic semiconductors for potential device applications. Doping with [6,6]-phenyl-C(61) butyric acid methyl ester leads to a red shift in the absorption only for 1 a and 2 a. An interesting NIR electrochromism was found for 2 a, with absorption being turned on at 1034 nm when electrochemically switched (at 1000 mV) from its neutral state to a radical cation state. Furthermore, a large Stokes shift (256-318 nm) is also unique for this multidonor-acceptor type of chromophore, indicating a significant structural difference between the ground state and the excited state. Photoluminescence of the film of 2 a was further probed at variable temperatures and the results strongly suggest that the restriction of bond rotations certainly helps to diminish non-radiative decay and thus enhance the luminescence of these large chromophores.
Drastic reduction of series resistance in doped semiconductor distributed Bragg reflectors for surface-emitting lasers (1990)

Modifications to reduce the series resistance in p-type semiconductor distributed Bragg reflectors (DBR) consisting of ten pairs of quarter-wavelength GaAs (high refractive index)/Al0.7Ga0.3As (low index) layers were made by inserting an intermediate Al0.35Ga0.65As layer or a 200 Å superlattice of GaAs(10 Å)/Al0.7Ga0.3As (10 Å) at the GaAs/Al0.7Ga0.3As heterointerfaces. The specific DBR series resistance was reduced by two orders of magnitude to about 6.2×10−5 Ω cm2. These modifications did not alter the optical reflectivity and nearly identical reflection spectra were measured.
Pure paclitaxel nanoparticles: preparation, characterization, and antitumor effect for human liver cancer SMMC-7721 cells (2018)

All conclusions verified that electrostatic spraying is a potential technology for developing PPN, and PPN can be regarded as a promising treatment for cancer.
High flux coherent super-continuum soft X-ray source driven by a single-stage, 10mJ, Ti:sapphire amplifier-pumped OPA (2014)

We demonstrate the highest flux tabletop source of coherent soft X-rays to date, driven by a single-stage 10 mJ Ti:sapphire regenerative amplifier at 1 kHz. We first down-convert the laser to 1.3 µm using a parametric amplifier, before up-converting it to soft X-rays using high harmonic generation in a high-pressure, phase matched, hollow waveguide geometry. The resulting optimally phase matched broadband spectrum extends to 200 eV, with a soft X-ray photon flux of > 10(6) photons/pulse/1% bandwidth at 1 kHz, corresponding to > 10(9) photons/s/1% bandwidth, or approximately a three order-of-magnitude increase compared with past work. Finally, using this broad bandwidth X-ray source, we demonstrate X-ray absorption spectroscopy of multiple elements and transitions in molecules in a single spectrum, with a spectral resolution of 0.25 eV, and with the ability to resolve the near edge fine structure.