gebhart/patrepeval huggingface dataset:
Near-infrared photothermal/photodynamic therapy with indocyanine green induces apoptosis of hepatocellular carcinoma cells through oxidative stress (2017)

Indocyanine green (ICG) is a photothermal agent, photosensitizer, and fluorescence imaging probe which shows specific accumulation in hepatocellular carcinoma (HCC) cells. We recently developed a photodynamic therapy (PDT) using ICG and near-infrared (NIR) laser as a new anti-cancer treatment for HCC. However, the molecular mechanism underlying this effect needs to be elucidated. HuH-7 cells, a well-differentiated human HCC cell line, were transplanted subcutaneously into BALB/c-nu/nu mice for in vivo experiment. ICG was administered 24 h before NIR irradiation. The irradiation was performed at three tumor locations by 823-nm NIR laser on days 1 and 7. The temperature of HuH-7 xenografts increased to 48.5 °C 3 minutes after ICG-NIR irradiation start. Reactive oxygen species (ROS) production was detected after ICG-NIR irradiation both in vitro and in vivo. There was certain anti-tumor effect and ROS production even under cooling conditions. Repeated NIR irradiation increased the cell toxicity of ICG-NIR therapy; the mean tumor volume on day 9 was significantly smaller after ICG-NIR irradiation compared to tumor without irradiation (87 mm<sup>3</sup> vs. 1332 mm<sup>3</sup>; p = 0.01) in HCC mice xenografts model. ICG-NIR therapy induced apoptosis in HCC cells via a photothermal effect and oxidative stress. Repeated ICG-NIR irradiation enhanced the anti-tumor effect.
Earthquake shakes Twitter users (2010)

Twitter, a popular microblogging service, has received much attention recently. An important characteristic of Twitter is its real-time nature. For example, when an earthquake occurs, people make many Twitter posts (tweets) related to the earthquake, which enables detection of earthquake occurrence promptly, simply by observing the tweets. As described in this paper, we investigate the real-time interaction of events such as earthquakes in Twitter and propose an algorithm to monitor tweets and to detect a target event. To detect a target event, we devise a classifier of tweets based on features such as the keywords in a tweet, the number of words, and their context. Subsequently, we produce a probabilistic spatiotemporal model for the target event that can find the center and the trajectory of the event location. We consider each Twitter user as a sensor and apply Kalman filtering and particle filtering, which are widely used for location estimation in ubiquitous/pervasive computing. The particle filter works better than other comparable methods for estimating the centers of earthquakes and the trajectories of typhoons. As an application, we construct an earthquake reporting system in Japan. Because of the numerous earthquakes and the large number of Twitter users throughout the country, we can detect an earthquake with high probability (96% of earthquakes of Japan Meteorological Agency (JMA) seismic intensity scale 3 or more are detected) merely by monitoring tweets. Our system detects earthquakes promptly and sends e-mails to registered users. Notification is delivered much faster than the announcements that are broadcast by the JMA.
A New Family of High-Affinity Transporters for Adenine, Cytosine, and Purine Derivatives in Arabidopsis (2000)

In many organisms, including plants, nucleic acid bases and derivatives such as caffeine are transported across the plasma membrane. Cytokinins, important hormones structurally related to adenine, are produced mainly in root apices, from where they are translocated to shoots to control a multitude of physiological processes. Complementation of a yeast mutant deficient in adenine uptake (fcy2) with an Arabidopsis cDNA expression library enabled the identification of a gene, AtPUP1 (for Arabidopsis thaliana purine permease1), belonging to a large gene family (AtPUP1 to AtPUP15) encoding a new class of small, integral membrane proteins. AtPUP1 transports adenine and cytosine with high affinity. Uptake is energy dependent, occurs against a concentration gradient, and is sensitive to protonophores, potentially indicating secondary active transport. Competition studies show that purine derivatives (e.g., hypoxanthine), phytohormones (e.g., zeatin and kinetin), and alkaloids (e.g., caffeine) are potent inhibitors of adenine and cytosine uptake. Inhibition by cytokinins is competitive (competitive inhibition constant K(i) = 20 to 35 microM), indicating that cytokinins are transported by this system. AtPUP1 is expressed in all organs except roots, indicating that the gene encodes an uptake system for root-derived nucleic acid base derivatives in shoots or that it exports nucleic acid base analogs from shoots by way of the phloem. The other family members may have different affinities for nucleic acid bases, perhaps functioning as transporters for nucleosides, nucleotides, and their derivatives.
Rapid saliva processing techniques for near real‐time analysis of salivary steroids and protein (2008)

No method was optimal for all analytes, highlighting the need for validation of saliva treatment methods before their adoption in rapid POC analyses.
Pharmacokinetic-pharmacodynamic relationships and dose response to meloxicam in horses with induced arthritis in the right carpal joint (2004)

Results of this study suggest that meloxicam is a potent anti-inflammatory drug in horses. A dosage of 0.6 mg/kg/d would be appropriate for use in a clinical study.
ID based public key cryptosystems based on Okamoto and Tanaka's ID based one way communication scheme (1990)

With a slight modification to Okamoto and Tanaka's ID-based one-way communication scheme, two identity-based public-key cryptosystems are proposed. These systems do not possess the conspiracy problem and can withstand certain key attacks common to most public-key cryptosystems.
Electrophilic Aromatic Substitution Reactions of 1,2-Dihydro-1,2-azaborines (2007)

The aromatic boron-nitrogen heterocycle 1,2-dihydro-1,2-azaborine undergoes classical electrophilic substitution. These reactions allow easy functionalization to provide a variety of 3- and 5-substituted derivatives. [reaction: see text].
Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells (2014)

Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow. Several studies have shown that mechanical stimulation modulates MSC differentiation through mobilization of second messengers, but the mechanism of mechanotransduction remains poorly understood. In this study, using fluorescence and laser confocal microcopy as well as patch-clamp techniques, we identified the transient receptor potential melastatin type 7 (TRPM7) channel as the key channel involved in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca(2+) increase and pressure-induced osteogenesis. TRPM7 directly sensed membrane tension, independent of the cytoplasm and the integrity of cytoskeleton. Ca(2+) influx through TRPM7 further triggered Ca(2+) release from the inositol trisphosphate receptor type 2 on the endoplasmic reticulum and promoted NFATc1 nuclear localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation-induced osteogenesis.
Molecular and crystal structure of 2-{(E)-[(4-Methylphenyl)imino]methyl}-4-nitrophenol: A redetermination (2016)

The crystal structure of the title compound, C14H12N2O3, was recently determined as a mixture of its neutral (OH containing) and zwitterionic (NH containing) forms, in a 0.60 (4): 0.40 (4) ratio using the X-ray determination. In this study, the title compound has been characterized by FT-IR and X-ray diffraction. The redetermination showed that the title compound has only enol (OH) form because of lack of the NH stretching vibration in FT-IR spectrum. In addition, the molecular structure and tautomerism of the title compound have been discussed.
Multi-beam digital antenna for radar, communications, and UAV tracking based on off-the-shelf wireless technologies (2006)

The state-of-art technologies keep generating new ways of improving on the performance of the old systems. Array antennas, one of the continuously improving technologies, brought many benefits to our life. The superiorities of array antennas remove the disadvantages of the old technology radars such as great sidelobes, vulnerability to the jammers, and degradation effect of the clutter. Array antennas find many applications on different areas. Today, unmanned aerial vehicles (UAVs) have begun to be seen in our life more often than before. UAVs prevent pilot loss of life. They carry out a variety of military and civilian missions such as surveillance and reconnaissance, target recognition, battle damage assessment, EW, search and rescue, and traffic monitoring. An important use of the UAVs is troop support, carrying out reconnaissance and surveillance missions, which requires maintaining a data-link with troops in order to send any data collected, such as video images, or audio. During operations it is necessary to continuously maintain a data and control link with the operator. This requires the ground station antenna to track the UAV so the antenna beam is pointed properly. The purpose of this research is to design and build an array to angle-track a UAV and, eventually, to accomplish the data transfer from the UAV to the ground station.
In Situ Real‐Time Chemiluminescence Imaging of Reactive Oxygen Species Formation from Cardiomyocytes (2008)

We have applied the highly sensitive chemiluminescence (CL) imaging technique to investigate the in situ ROS formation in cultured monolayers of rat H9c2 cardiomyocytes. Photon emission was detected via an innovative imaging system after incubation of H9c2 cells in culture with luminol and horseradish peroxidase (HRP), suggesting constitutive formation of ROS by the cardiomyocytes. Addition of benzo(a)pyrene-1,6-quinone (BPQ) to cultured H9c2 cells resulted in a 4-5-fold increase in the formation of ROS, as detected by the CL imaging. Both constitutive and BPQ-stimulated CL responses in cultured H9c2 cells were sustained for up to 1 hour. The CL responses were completely abolished in the presence of superoxide dismutase and catalase, suggesting the primary involvement of superoxide and hydrogen peroxide (H2O2). In contrast to BPQ-mediated redox cycling, blockage of mitochondrial electron transport chain by either antimycin A or rotenone exerted marginal effects on the ROS formation by cultured H9c2 cells. Upregulation of cellular antioxidants for detoxifying both superoxide and H2O2 by 3H-1,2-dithiole-3-thione resulted in marked inhibition of both constitutive and BPQ-augmented ROS formation in cultured H9c2 cells. Taken together, we demonstrate the sensitive detection of ROS by CL imaging in cultured cardiomyocytes.
Colonyzer: automated quantification of micro-organism growth characteristics on solid agar (2010)

Colonyzer can automatically quantify culture growth from large batches of captured images of microbial cultures grown during genome-wide scans over the wide range of cell densities observable after highly dilute liquid spot inoculation, as well as after more concentrated pinning inoculation. Colonyzer is open-source, allowing users to assess it, adapt it to particular research requirements and to contribute to its development.
Oxidant Stress and Reduced Antioxidant Enzyme Protection in Polycystic Kidney Disease (2002)

Oxidative stress has been implicated in the pathogenesis of both acquired and hereditary polycystic kidney disease. Mechanisms of oxidant injury in C57BL/6J-cpk mice and Han:SPRD-Cy rats with rapidly or slowly progressive polycystic kidney disease were explored. Expression of heme oxygenase-1 mRNA, an inducible marker of oxidative stress, was shown to be increased in cystic kidneys of mice and rats in a pattern that reflected disease severity. By contrast, there was a decrease in mRNA expression of the antioxidant enzymes extracellular glutathione peroxidase, superoxide dismutase, catalase, and glutathione S-transferase during disease progression. Renal mRNA levels of these enzymes were strikingly reduced in rapidly progressive disease in homozygous cystic mice and rats. In slowly progressive disease in heterozygous rats, renal antioxidant mRNA levels were decreased to a greater extent in cystic males than in the less severely affected females. Protein levels for extracellular glutathione peroxidase were also reduced in plasma and in cystic kidneys of mice and rats. Plasma extracellular glutathione peroxidase enzymatic activity was also decreased, whereas the lipid peroxidation products malondialdehyde and 4-hydroxy-2(E)-nonenal were increased in kidneys and blood plasma of cystic mice. Reduced antioxidant enzyme protection and increased oxidative damage represent general mechanisms in the pathogenesis of polycystic kidney disease.
HMM clustering for connected word recognition (2003)

The authors describe an HMM (hidden Markov model) clustering procedure and discuss its application to connected-word systems and to large-vocabulary recognition based on phonelike units. It is shown that the conventional approach of maximizing likelihood is easily implemented but does not work well in practice, as it tends to give improved models of tokens for which the initial model was generally quite good, but does not improve tokens which are poorly represented by the initial model. The authors have developed a splitting procedure which initializes each new cluster (statistical model) by splitting off all tokens in the training set which were poorly represented by the current set of models. This procedure is highly efficient and gives excellent recognition performance in connected-word tasks. In particular, for speaker-independent connected-digit recognition, using two HMM-clustered models, the recognition performance is as good as or better than previous results using 4-6 models/digit obtained from template-based clustering.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Experimental Study of Fretting Crack Nucleation in Aerospace Alloys with Emphasis on Life Prediction (2000)

A statistically-designed experimental program engendered to validate an analytical approach for the prediction of fretting crack nucleation in 2024-T351 aluminum alloy has been completed. The test results indicate that the near-surface cyclic contact stress and strain field can be juxtaposed with a multiaxial fatigue life parameter relying on uniaxial strain-life constants to predict crack nucleation for a wide range of load intensities and conditions representative of those experienced in riveted joints. With this approach validated, efforts have been initiated to predict fretting-induced fatigue failures in riveted single lap joint structures. Research was targeted at characterizing the conditions at and around the rivet/hole interface, including finite element modeling of both the mechanics of load transfer in riveted joints and the residual stress field introduced during the rivet installation process. Model results and an ancillary set of fatigue tests of single lap joint test articles have identified a strong correlation among riveting process parameters, the mechanics of load transfer, and the subsequent tribological and fatigue degradation of the joints. Final comments are offered regarding the ability of this integrated approach to predict the fatigue performance of riveted lap joint structures.
Data analysis in extended x-ray-absorption fine structure: Determination of the background absorption and the threshold energy (1983)

Two approaches for the determination of the background absorption (${\ensuremath{\mu}}_{0}$) in the extended x-ray-absorption fine structure (EXAFS) are presented. Both methods, experimental and computational, take advantage of the damping of the EXAFS amplitude resulting from the convolution with Gaussian functions of different widths. In the experimental method two or more spectra are collected with the use of different spectrometer slit widths, resulting in spectra of different resolutions for the same sample. In the computational approach the convolution is accomplished via a convolution algorithm. The intersection points of the resulting spectra are used to generate ${\ensuremath{\mu}}_{0}$. At the absorption edge, the spectra intersect at a unique point, which is shown to be a measure of the threshold energy, ${E}_{0}$. Illustration of the two methods for background removal is given for a copper-foil sample. The computational approach is superior to the experimental method of damping the EXAFS spectra to give ${\ensuremath{\mu}}_{0}$.
Epidermal growth factor (EGF) receptor blockade inhibits the action of EGF, insulin-like growth factor I, and a protein kinase A activator on the mitogen-activated protein kinase pathway in prostate cancer cell lines. (1999)

Epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) are potent mitogens that regulate proliferation of prostate cancer cells via autocrine and paracrine loops and promote tumor metastasis. They exert their action through binding to the corresponding cell surface receptors that initiate an intracellular phosphorylation cascade, leading to the activation of mitogen-activated protein kinases (MAPKs), which recruit transcription factors. We have studied the effects of EGF, IGF-I, and the protein kinase A (PKA) activator forskolin on the activation of p42/ extracellular signal-regulated kinase (ERK)2, which is a key kinase in mediation of growth factor-induced mitogenesis in prostate cancer cells. The activity of p42/ERK2 was determined by immune complex kinase assays and by immunoblotting using a phospho p44/p42 MAPK-specific antibody. EGF, IGF-I, and forskolin-induced PKA activity stimulate intracellular signaling pathways converging at the level of p42/ERK2. In the androgen-insensitive DU145 cell line, there is a constitutive basal p42/ ERK2 activity that is not present in androgen-sensitive LNCaP cells. Constitutive p42/ERK2 activity is abrogated by blockade of the EGF receptor. Hence, it is obviously caused by an autocrine loop involving this receptor. The effects of EGF on p42/ERK2 are potentiated by forskolin in both cell lines. The blockade of PKA by the specific inhibitor H89 attenuates this synergism. This finding is in contrast to those obtained in several other systems studied thus far, in which PKA activators inhibited MAPKs. p42/ERK2 in DU145 cells is highly responsive to IGF-I stimulation, whereas no effect of IGF-I on p42/ERK2 can be measured in LNCaP cells. Moreover, our results demonstrate that selective blockade of the EGF receptor in prostate cancer cells does not only inhibit the action of EGF, but also IGF-I-induced activation of the MAPK pathway and the interaction with the PKA pathway. In conclusion, these findings offer new possibilities for a therapeutical intervention in prostate cancer by targeting signaling pathways of growth factors and PKA.
Does Snoring Predict Sleepiness Independently of Apnea and Hypopnea Frequency? (2000)

Obstructive apneas and hypopneas during sleep are a well recognized cause of excessive daytime sleepiness. Snoring is also associated with excess sleepiness, although it is not known whether this reflects an independent effect of snoring or whether snoring is simply a marker for obstructive sleep apnea. To further explore the relation of snoring to sleepiness, we conducted a cross-sectional cohort study of community-dwelling adults participating in the Sleep Heart Health Study. The study sample comprises 2,737 men and 3,040 women with a mean age of 64 (SD 11) yr. Sleepiness was quantified using the Epworth Sleepiness Scale (ESS). Snoring history was obtained via a self-completion questionnaire. The respiratory disturbance index (RDI), defined as the number of apneas plus hypopneas per hour of sleep, was measured during in-home polysomnography. The ESS score increased progressively with increasing RDI, from a mean of 7.1 (4.2) in subjects with RDI < 1.5 to 8.8 (4.8) in subjects with RDI >/= 15 (p < 0.001). A progressive increase in ESS score was also seen across five categories of snoring frequency, from 6.4 (4.2) in current nonsnorers to 9.3 (4.8) in subjects who snored six to seven nights per week (p < 0.001). The prevalence of excessive daytime sleepiness, defined as an ESS score >/= 11, increased from 15% in never-snorers to 39% in those who snored six to seven nights per week. The relation of snoring to sleepiness was seen at all levels of RDI, with no significant change in the relation of snoring to ESS score after adjustment for RDI in multivariate models. The effects of snoring and RDI on sleepiness were little affected by adjustment for age, sex, race, body mass index, or questionnaire evidence of insufficient sleep time or nocturnal leg jerks or cramps. We conclude that both snoring and RDI are independently associated with excess sleepiness in community-dwelling, middle-aged and older adults.
Vegetable Diseases (2006)

Our dependence on healthy vegetable crops as a reliable source of food transcends all barriers of nation and culture. Consumers now demand excellent quality from the industry that produces large volumes of high quality vegetables to be sold locally, regionally and shipped internationally. The diseases that affect vegetables compromise such quality