gebhart/patrepeval huggingface dataset:
Stepwise amplified immunoperoxidase (PAP) staining. I. Cellular morphology in relation to membrane markers. (1984)

A novel procedure for the assay of monoclonal antibodies is described. The technique is based on a combination of three principles. Unlabeled (sheep) antiserum to mouse immunoglobulin (Ig) and complexes of peroxidase with mouse monoclonal antiperoxidase (monoclonal PAP complexes) are used as reagents in a variant of the unlabeled antibody enzyme (PAP) method, described by Sternberger. The amount of peroxidase eventually bound to a monoclonal antibody can be varied over a wide range by repetition of incubation cycles with anti-mouse Ig and monoclonal PAP complexes. During the assay, incubations and wash steps are performed by immersion of whole slides. The influence of repetitive incubation cycles with anti-mouse Ig and monoclonal PAP complexes on background staining and detection of monoclonal antibodies at low concentrations was quantitated in a model system. At a given primary antibody concentration, a linear relationship was found between peroxidase activity and the number of incubation cycles. Application of the technique to the detection of monoclonal antibodies bound to cell-surface antigens is described. Peripheral blood cells were labeled in suspension with monoclonal antibodies. Cytocentrifuge preparations of labeled cells were prepared, and such preparations were fixed before stepwise-amplified PAP staining. Cells showed intense specific staining. Morphological detail of stained and unstained cells is preserved, allowing morphological analysis of labeled cells and rapid analysis of monoclonal antibody specificity. Because the reagents used in the assay can be produced in large quantities with uniform quality, the technique can be readily automated. This, together with the possibility to increase the sensitivity of antibody detection in a controlled, stepwise fashion to levels that cannot be reached with "single-step" techniques, may further expand the applications of monoclonal antibodies.
Effects of salicylate and 2,4-dinitrophenol on respiration and metabolism (1982)

The respiratory and metabolic responses to intravenous administration of sodium salicylate (240 mg/kg0.75) and 2,4-dinitrophenol (5.7 mg/kg0.75), uncouplers of oxidative phosphorylation, were studied in anesthetized, paralyzed, vagotomized, and glomectomized cats. During the initial 10 min after administration of salicylate, metabolic rate (VCO2) increased 70% and did not increase further during the experiment. Respiration, on the other hand, increased progressively at the same rate over the entire 60-min course of the experiment. The total increase in respiration was greater than 300%. Thus neither the time course nor the magnitude of the metabolic and respiratory responses were related. Similar findings were also obtained following administration of 2,4-dinitrophenol. We conclude that these agents stimulate respiration by a mechanism other than one related to the ability to uncouple oxidative phosphorylation and cause an increase in whole-body metabolism.
Experimental Studies on Reflection Spectra in Monodomain Cholesteric Liquid Crystal Cells: Total Reflection, Subsidiary Oscillation and Its Beat or Swell Structure (1983)

Many optical properties are presented here in the reflection spectra for monodomain cholesterics of various cell thicknesses at various angles of incidence. It is reported for the first time that left-circularly polarized light incident on a left-handed helix gives rise to a beat structure in the subsidiary oscillation, while right-circularly polarized light causes a swell to be superimposed on the subsidiary oscillation. The beat structure is ascribed to the excitation of two sets of optical eigen modes, each set consisting of two optical eigen modes of slightly different wavelength. The polarization characteristics of the total reflection band as well as the selective reflection band are also discussed.
A dual-stage low-power converter driving for piezoelectric actuator applied in micro robot (2019)

In this article, a dual-stage converter driving for a piezoelectric actuator based on flyback circuit was designed and implemented, which could be applied in a micro robot. A low-voltage direct current could be converted to a high-voltage alternating current through flyback circuit and direct current/alternating current circuit in low-power condition. In the direct current/direct current stage, the charging and discharging process was realized to generate a high voltage bias from a low voltage directly supplied by battery. Then, the high voltage was converted into alternating waveform by an energy recovery circuit in direct current/alternating current stage. Experiments were conducted to verify the ability of the proposed converter to drive a 100-V-input piezoelectric bimorph actuator using a prototype 108 mg (excluding printed circuit board mass), 169 (13 × 13) mm 2 , and 500-mW converter. According to the experimental results, this converter could be used for driving piezoelectric actuator applied in micro robot.
Cyclic Dimethylsiloxanes as Pseudo Crown Ethers: Syntheses and Characterization of Li(Me<sub>2</sub>SiO)<sub>5</sub>[Al{OC(CF<sub>3</sub>)<sub>3</sub>}<sub>4</sub>], Li(Me<sub>2</sub>SiO)<sub>6</sub>[Al{OC(CF<sub>3</sub>)<sub>3</sub>}<sub>4</sub>], and Li(Me<sub>2</sub>SiO)<sub>6</sub>[Al{OC(CF<sub>3</sub>)<sub>2</sub>Ph}<sub>4</sub>] (2006)

Ein unerwarteter Gast: Die Salze LiD5[AlF], LiD6[AlF] und LiD6[AlPhF] (Dn=(Me2SiO)n; [AlF]=Al{OC(CF3)3}4; [AlPhF]=Al{OC(CF3)2Ph}4) sind die ersten Wirt-Gast-Komplexe, die direkt aus cyclischen Dimethylsiloxanen, Alkalimetallionen und schwach koordinierenden Anionen synthetisiert wurden. Nach den experimentell und rechnerisch bestimmten Strukturen binden die cyclischen Dimethylsiloxane als Pseudo-Kronenether (siehe Struktur von [LiD6]+). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2001/2006/z504262_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Loudness of complex signals presented by electrical stimulation of the auditory nerve (1985)

An understanding of the factors affecting the loudness of complex electrical stimulation is necessary if improved speech processing schemes are to be developed for cochlear implants. This experiment used a variety of complex signal types to examine the relationship between perception of loudness and physical characteristics of electrical stimulation. Loudness was studied for a set of 19 stimuli differing in bandwidth, center frequency, peak factor, spectral shape, and tonality. Four cochlear implant subjects and two normal-hearing subjects made equal-loudness judgments for the stimuli using the method of adjustment. A 200-Hz tone at a comfortable listening level served as the reference. Equated stimuli delivered to the electrode were analyzed for amplitude and frequency characteristics. The relationship of these statistics to perceived loudness will be discussed.
Towards Optimised Context Selection In Scalable Wavelet Based Video Coding (2007)

Publication in the conference proceedings of EUSIPCO, Poznan, Poland, 2007
Untersuchungen über den Halogen‐Halogen‐Austausch am aromatischen Kern, III. Chlorierung von Fluorbenzol (1958)

Abstract Die Endprodukte der Chlorierung von Fluorbenzol in Gegenwart von Katalysatoren sind je nach Versuchsbedingungen Hexachlorbenzol bzw. Hexachlor‐ und Pentachlor‐monofluor‐benzol.
A fuzzy logic based speech extraction approach for e-Learning content production (2008)

This paper proposed a fuzzy logic based approach to label speech segments from media files for content production in e-Learning systems. A fuzzy inference system (FIS) was introduced to fuzzify the extracted features extracted from different dimensions. apply predefined rules, and generate an output that represents the degree a clip belonging to speech. We show that our methodology improves the accuracy of the speech detection, and enhances the efficiency and robustness of the voice activity detection (VAD), comparing with the single feature based VAD algorithms.
Plasma kallikrein during experimentally induced allergic rhinitis: role in kinin formation and contribution to TAME-esterase activity in nasal secretions. (1986)

We have shown recently that kinins are generated during experimentally induced allergic rhinitis in man, and have demonstrated that substrates for kinin-forming enzymes are provided during the allergic response by a transudation of kininogens from plasma into nasal secretions. In light of this increased vascular permeability during the allergic reaction, we have extended our studies on the mechanisms of kinin formation to examine the potential involvement of plasma kallikrein. Allergic individuals (n = 7) and nonallergic controls (n = 7) were challenged intranasally with an allergen, and nasal lavages, obtained before and after challenge, were assayed for immunoreactive human plasma kallikrein/prekallikrein (iHPK). Post-challenge iHPK values were significantly elevated (p less than 0.01) in the allergic group (353 +/- 394 ng/ml; x +/- SD) as compared to the nonallergics (19 +/- 22 ng/ml), and correlated with increases in kinins, histamine, and N-alpha-tosyl-L-arginine methyl esterase (TAME-esterase) activity and with the onset of clinical symptoms. Gel filtration studies revealed that plasma prekallikrein is activated during the allergic response and contributes to kinin formation prior to interaction with plasma protease inhibitors. We also show that the majority of the TAME-esterase activity detected in nasal secretions during the allergic response is due to activities consistent with a plasma kallikrein/alpha 2-macroglobulin complex and with mast cell tryptase.
Bias in the cross spectrum and time delay estimates due to misalignment (1986)

The effect of misalignment in the weighted, overlapped segment averaging method used for estimating time delay is investigated. With nonwhite input, it is shown that the phase of the cross spectrum and the location of the peak in the cross correlator are biased.
Stimulation of repair in chronic, nonhealing, cutaneous ulcers using platelet-derived wound healing formula. (1990)

Chronic, nonhealing, cutaneous ulcers are a serious clinical problem. The results of previous studies using platelet-derived wound healing formula (PDWHF), derived from autologous platelets, provided evidence that PDWHF actively stimulates repair of the wound. To test whether or not PDWHF accelerates repair, a prospectively randomized, blinded trial was conducted using a placebo control. A total of 32 patients with chronic, nonhealing, cutaneous wounds of the lower extremity were randomized and treated for eight weeks with PDWHF or placebo. Epithelialization of the wound was the end point of study. In the group who received treatment, 81 per cent of patients had epithelialization in eight weeks compared with 15 per cent in the control group (p less than 0.0001). After crossover to treatment with PDWHF, all of the patients in the control group had epithelialization in an average of 7.1 weeks. Regression analysis of the rates of epithelialization also showed significant differences during the initial eight week trial and showed no difference after crossover of the control group to therapy with PDWHF. Results from this study demonstrate a highly statistically significant effect of topically applied platelet-derived growth factors on the repair of chronic, nonhealing, cutaneous ulcers.
Safety and efficacy of micronized tretinoin gel (0.05%) in treating adolescent acne. (2011)

Tretinoin is widely used in the treatment of acne. Despite significant advances in formulation development, irritation and dryness can be particularly bothersome, especially during the first 3-4 weeks, impacting adherence. Dose titration and adjunct use of moisturizers have been commonly employed. Co-prescribing with benzoyl peroxide (BPO) or a BPO/antibiotic combination is also common practice. The tretinoin molecule is unstable and can be degraded by BPO, further complicating treatment regimens. Lately, formulation technology has focused on providing more efficient penetration of the tretinoin into the skin layers so that lower concentrations of tretinoin might afford better tolerability, but maintain good efficacy; incorporating moisturizing excipients to minimize irritation; and providing greater stability to the tretinoin molecule. This approach would be particularly relevant in a pediatric acne population where efficacy/tolerability balance is important and treatment regimens must take into account lifestyles, but little data exist on the use of tretinoin in this patient population. A micronized formulation of tretinoin (0.05%) gel has been developed that provides a more efficient delivery of tretinoin, because of its optimal particle size, no degradation by BPO and better cutaneous tolerability than tretinoin microsphere (0.1%) gel without compromising efficacy in a pediatric population.
Peroxidase‐catalyzed co‐oxidation of indole‐3‐acetic acid and xanthene dyes in the absence of hydrogen peroxide (1993)

The effect of xanthene dyes on the chemiluminescence from the aerobic indole-3-acetic acid (IAA) oxidation, catalyzed by horseradish peroxidase (HRP), was studied. The rate of IAA oxidation and dye destruction were controlled. It was found that the addition of dyes to the IAA/HRP/O2 system resulted in: (i) the appearance of emission in the region of dye fluorescence, (ii) an increase of the total chemiluminescence intensity, (iii) a decrease of the emission duration, (iv) the acceleration of IAA oxidation, and (v) slow bleaching of the dyes. The experimental results lead to the conclusion that all spectral and kinetic variations of the chemiluminescence from the IAA/HRP/O2 system which are caused by the addition of xanthene dyes, are the result of IAA-dye co-oxidation. Earlier published reports regarding energy transfer from electronically excited species, generated in the IAA/HRP/O2 system, to the xanthene dyes seem to be erroneous.
Model for the Thermodynamic Properties of a Biodiesel Fuel (2009)

We present an equation-of-state approach to modeling the thermodynamic properties of a biodiesel fuel. Preliminary Helmholtz-type equations of state were developed with limited experimental data for five fatty acid methyl esters (FAMEs) that are the primary constituents of soy-based biodiesel fuel, namely, methyl palmitate, methyl stearate, methyl oleate, methyl linoleate, and methyl linolenate. These components were combined in a mixture model using ideal mixing to represent the thermodynamic properties of a biodiesel fuel. We performed limited experimental measurements on the density, sound speed, and initial boiling point of two biodiesel fuel samples and compared the results with the model.
Monolithic integration of a 10 Gb/s Mach-Zehnder modulator and a widely tunable laser based on a 2-ring loop-filter (2010)

A 10-Gb/s Mach-Zehnder modulator is monolithically integrated with a tunable laser source based on a 2-ring loop-filter with a small device dimension (2.5 × 0.9 mm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ). Sufficient static extinction ratio of 16.5 dB was obtained both at 1573.92 and 1595.50 nm.
Surface Modification of Steel Tire Cords via Plasma Etching and Plasma Polymerization Coating : Part II. Characterization (2000)

Zinc plated steel tire cords were subjected to RF plasma etching of argon, followed by plasma polymerization coating of acetylene or butadiene in order to enhance adhesion to rubber compounds. Plasma polymerization was carried out under optimized conditions of 10 W, 30 sec, 30 mTorr for acetylene and butadiene gas, while plasma etching was performed at 90W, 10min and 30mTorr. The adhesion of tire cords was evaluated via Tire Cords Adhesion Test (TCAT) and the failure surfaces of the tested samples were analyzed by SEM. Polymer coating by plasma polymerization was also characterized by FT-IR, Alpha-Step and dynamic contact angle analyzer in order to elucidate the adhesion mechanism.
Temporal Dynamics for Blind Measurement of Room Acoustical Parameters (2010)

In this paper, short- and long-term temporal dynamic information is investigated for the blind measurement of room acoustical parameters. In particular, estimators of room reverberation time (T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">60</sub> ) and direct-to-reverberant energy ratio (DRR) are proposed. Short-term temporal dynamic information is obtained from differential (delta) cepstral coefficients. The statistics computed from the zeroth-order delta cepstral sequence serve as input features to a support vector T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">60</sub> estimator. Long-term temporal dynamic cues, on the other hand, are obtained from an auditory spectrotemporal representation of speech commonly referred to as modulation spectrum. A measure termed as reverberation-to-speech modulation energy ratio, which is computed per modulation frequency band, is proposed and serves as input to T <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">60</sub> and DRR estimators. Experiments show that the proposed estimators outperform a baseline system in scenarios involving reverberant speech with and without the presence of acoustic background noise. Experiments also suggest that estimators of subjective perception of spectral coloration, reverberant tail effect, and overall speech quality can be obtained with an adaptive speech-to-reverberation modulation energy ratio measure.
Effect of glycerol, polyethylene glycol and glutaraldehyde on stability of phenylalanine ammonia-lyase activity in yeast (1987)

The polyhydric alcohols, glycerol and sorbitol, significantly increased the rate of e-phenylalanine production from trans-cinnamic acid using whole cells of Rhodotorula rubra. Chloride ions and oxygen prevented the stimulatory effect of the polyhydric alcohols. Furthermore, the severe inhibition of the biotransformation by high trans-cinnamic acid concentrations was alleviated in the presence of glycerol and sorbitol. The rate of conversion could be manipulated still further, even with high trans-cinnamic acid concentrations, by elevating the reaction pH to 12 in the presence of polyhydric alcohol. When ceils were also treated first with glutaraldehyde (0.1% v/v) and then polyethylene glycol (15% v/v), although neither compound stimulated the actual rate of bioconversion, the reaction was markedly stabilised and gave a 73% yield after 28 days of continuous operation.
MD-Adapt: A Proposed Architecture for Open-Source Medical Device Interoperability (2007)

MD-adapt is an architecture for shamble common interfaces for medical devices in support of the goals of MDPnP. This paper proposes an architecture for enabling an open source, community-based effort to create a pool of reusable device interface implementations. The MD-adapt architecture consists of a standardized API (application programming interface) and a device metadata format, and supports building modular and portable medical device communications software adapters that run on a variety of relevant OS platforms.
Radiosensitization of Human Bronchogenic Carcinoma Cells by Interferon Beta (1984)

The effects of interferons on the radiosensitivity of in vitro human bronchogenic carcinoma cells was investigated. Human fibroblast-derived interferon (IFN-beta) was found to sensitize cells to gamma irradiation while either HuIFN-alpha or mouse IFN-alpha/beta did not. The observed radiosensitization was supra-additive and resulted in a decrease in the shoulder width of the radiation dose-cell survival curve but did not affect the slope. The degree of radiosensitization of the various IFNs tested paralleled the antiproliferative effects of these IFNs on this cell line.
Characterization of the 48.5 kDa Chorionic Gonadotropin-Like Protein from<i>Xanthomonas Maltophilia</i> (1993)

Our laboratory has previously reported the isolation of a 48.5 kDa membrane protein from Xanthomonas maltophilia (ATCC 13637) which immunologically cross-reacts with the beta-subunit of human chorionic gonadotropin (hCG) in both polyclonal and monoclonal radioimmunoassays (RIA) (1). The protein showed no reaction in RIAs for human LH, TSH, or the free alpha subunit of hCG. We have now improved the protein purification procedure and have obtained adequate bacterial protein to characterize the pure protein (designated xCG) in RIAs and also to obtain amino acid composition and partial sequence. We present these data and compare homology to hCG. An RIA for the bacterial protein has also been developed.
CHANGES IN THE LEVELS OF DNA, RNA, PROTEIN AND DNases IN DEVELOPING AND OLD CHICK BRAIN (1975)

Abstract —The levels of DNA, RNA, protein and activities of acid and alkaline DNases in developing and old chicken brain were studied. A rapid increase in DNA content was found in the embryonic brain until just prior to hatching. Thereafter, with a transient plateau around the day of hatching, the DNA continued to increase but at a very slow rate. Two‐year‐old brain was found to have a markedly higher level of DNA as compared to the 10th day postnatal value. RNA showed a steady increase up to the 20th day of embryonic life. Protein levels showed a gradual increase throughout the period studied. Both acid and alkaline DNases exhibited maximum activity during embryonic life, i.e. at a time when rapid cellular proliferation was occurring. With advancing age, the acid DNase activity showed a marked decline thus exhibiting no correlation to the high level of DNA found in the old brain. The alkaline DNase activity, however, was still at a significant level in the 2‐year‐old brain.
The Preparation and Properties of Some New Nitrogen and Fluorine Derivatives of Disilane (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation and Properties of Some New Nitrogen and Fluorine Derivatives of DisilaneMansour Abedini and Alan G. MacDiarmidCite this: Inorg. Chem. 1963, 2, 3, 608–613Publication Date (Print):June 1, 1963Publication History Published online1 May 2002Published inissue 1 June 1963https://doi.org/10.1021/ic50007a046Request reuse permissionsArticle Views85Altmetric-Citations29LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (773 KB) Get e-Alertsclose Get e-Alerts
The Impact of Ineffective and Inefficient Care on the Excess Costs of Elective Surgical Procedures (2011)

Inefficiency is substantially more important than suboptimal outcomes in accounting for the excessive hospital costs of elective surgical care in this study population.