gebhart/patrepeval huggingface dataset:
Intercellular spread of Shigella flexneri through a monolayer mediated by membranous protrusions and associated with reorganization of the cytoskeletal protein vinculin (1991)

The spread of Shigella flexneri in a monolayer of infected Henle and HeLa cells was studied by using immunofluorescence and electron microscopy. Infected cells produced numerous bacterium-containing membranous protrusions up to 18 microns in length that penetrated adjacent cells and were subsequently phagocytosed. Fluorescence staining of actin and vinculin in infected cells with phalloidin and monoclonal antibody to vinculin, respectively, demonstrated that the protrusions containing the bacteria consisted of these cytoskeletal proteins. Actin accumulated predominantly at the poles of bacteria distal to the tip of protrusions and appeared as trails extending back towards the host cell cytoplasm. Vinculin, however, was distributed uniformly around the bacteria and throughout the protrusion. A profound rearrangement of vinculin occurred in Henle and HeLa cells following infection with shigellae: whereas in uninfected cells it was distributed mainly around the cell periphery, in infected cells it concentrated mainly around clusters of bacteria in the cytoplasm. This suggests a possible involvement of the vinculin cytoskeletal protein in the intercellular spread of shigellae during an infection.
Requirements for Langerhans' cell depletion following in vitro exposure of murine skin to ultraviolet-B. (1993)

Langerhans' cells found within the skin and mucous membranes are critical regulators of antimicrobial and allergic responses. Therefore, the depletion of these cells following exposure of skin to solar ultraviolet radiation (UV) has direct functional consequences on immunity within this tissue. In order to understand how Langerhans' cell depletion is regulated following exposure of skin to medium-wave UV (UVB), the role of second messengers in these responses was investigated using a novel in vitro system. This was accomplished by analysing the expression of a specific marker associated with Langerhans' cells (ATPase) among the epidermal portion of cultured sections of mouse skin following treatment with inhibitors specific for second messenger components and subsequent exposure to UVB. In this study, inhibitors of guanosine triphosphate (GTP) binding proteins, H-8, pertussis toxin and cholera toxin as well as inhibitors of RNA and protein synthesis were all capable of blocking Langerhans' cell depletion in response to UVB treatment. In contrast, an inhibitor of protein kinase C (H-7) was incapable of specifically blocking depletion following treatment with this physical agent. These results suggest that Langerhans' cell depletion mediated by UVB may involve a pertussis and cholera toxin-sensitive G protein as well as de novo protein synthesis.
Three-Dimensional Geometry of the Tricuspid Annulus in Healthy Subjects and in Patients With Functional Tricuspid Regurgitation (2006)

Real-time 3D echocardiography showed a complicated 3D structure of the TA, which appeared to be different from the "saddle-shaped" mitral annulus, suggesting an annuloplasty for TR different from that for mitral regurgitation.
Aphid Transmission and Systemic Plant Infection Determinants of Barley Yellow Dwarf Luteovirus-PAV are Contained in the Coat Protein Readthrough Domain and 17-kDa Protein, Respectively (1996)

Proteins encoded by open reading frames (ORF) 3, 4, and 5 of the barley yellow dwarf luteovirus genome are translated from a single subgenomic RNA. The structural proteins are encoded by ORF 3 (coat protein) and ORF 5 (readthrough domain) and contain undefined domains that regulate the movement of virus through aphid vectors. The biological function of the nonstructural 17-kDa protein encoded by ORF 4 is unknown. A complementation method was employed to test the ability of barley yellow dwarf virions carrying mutations within the readthrough domain and the 17-kDa protein to be transmitted by aphids and to cause systemic infections in plants. We show that the readthrough domain is required for aphid transmission; however, it is not required for virus to be taken up by aphid hindgut cells and released into the hemocoel. The circulative pathway of luteoviruses in aphid vectors requires that virus be actively transported from the hemolymph into the salivary system. Thus, it appears that the readthrough domain is required for transport of virus through membranes of the aphid salivary glands. Furthermore, the readthrough domain was not required for systemic infection of plants, but did influence the accumulation of virus in infected plants. The 17-kDa protein is required for the systemic infection of plants.
NMR Structure and Mutagenesis of the Third Bir Domain of the Inhibitor of Apoptosis Protein XIAP (2000)

The inhibitor of apoptosis proteins (IAPs) regulate the caspase family of cysteine proteases, which play an important role in the execution of programmed cell death. Human X-linked inhibitor of apoptosis protein (XIAP) is a potent inhibitor of caspases-3, -7, and -9. Here we show that the Bir3 domain is the minimal region of XIAP that is needed for potent caspase-9 inhibition. The three-dimensional structure of the Bir3 domain of XIAP, determined by NMR spectroscopy, resembles a classical zinc finger and consists of five alpha-helices, a three-stranded beta-sheet, and a zinc atom chelated to three cysteines and one histidine. The structure of the Bir3 domain is similar to that of the Bir2 domain of XIAP but differs from the previously determined structure of the Bir3 domain of MIHB. Based on site-directed mutagenesis, we have identified the regions of the Bir3 domain of XIAP that are important for inhibiting caspase-9. Despite the structural similarities of the Bir2 and Bir3 domain of XIAP, a different set of residues were found to be critical for inhibiting the individual caspases. These results suggest that XIAP inhibits caspase-3 and caspase-9 in a different manner.
Nonlinear Refractory Plasmonics with Titanium Nitride Nanoantennas (2016)

Titanium nitride (TiN) is a novel refractory plasmonic material which can sustain high temperatures and exhibits large optical nonlinearities, potentially opening the door for high-power nonlinear plasmonic applications. We fabricate TiN nanoantenna arrays with plasmonic resonances tunable in the range of about 950-1050 nm by changing the antenna length. We present second-harmonic (SH) spectroscopy of TiN nanoantenna arrays, which is analyzed using a nonlinear oscillator model with a wavelength-dependent second-order response from the material itself. Furthermore, characterization of the robustness upon strong laser illumination confirms that the TiN antennas are able to endure laser irradiation with high peak intensity up to 15 GW/cm(2) without changing their optical properties and their physical appearance. They outperform gold antennas by one order of magnitude regarding laser power sustainability. Thus, TiN nanoantennas could serve as promising candidates for high-power/high-temperature applications such as coherent nonlinear converters and local heat sources on the nanoscale.
Reflective-type optical encoder based on fractional Talbot self-image effect using phase grating (2010)

This study presents a reflective-type optical encoder based on fractional Talbot self-image effect using a phase grating. An amplitude grating image is produced by the phase grating at a quarter or three quarters of Talbot distance. The encoder has the advantages of a large gap and more light efficiency. This work use a 850nm Light-Emitting Diode (LED) as light source, the light source will be collimated by a collimator ,and incident on a phase grating by 45° angle. The fractional Talbot self-image of phase grating is produced and projected on an amplitude index grating. The Moiré interference is occurred when the both gratings displaced from each other. The encoder includes a reflective-type phase-grating scale with period 20 μm and an index-amplitude grating. The gap between the phase index grating and mail scale was increased to three quarters of Talbot distance. The tolerance of gap between the index and main grating is 0.1 mm. The tolerance of yaw motion is ± 0.25°.
An assessment of two evanescent field biosensors in the development of an immunoassay for tuberculosis (2009)

Accurate diagnosis of active tuberculosis is required to improve treatment, reduce transmission of the disease and control the emergence of drug resistance. A rapid and reliable test would make a considerable contribution to the management of the TB epidemic, especially in HIV-burdened and resource-poor countries where access to diagnostic laboratories are limited. Surrogate marker antibody detection to mycobacterial lipid cell wall antigens gave promising results, in particular with cord factor. The specific advantage of using mycolic acids as lipid antigens in comparison to protein antigens is that mycolic acid is a CD1 restricted antigen with the ability to induce proliferation of CD4/CD8 double negative T-cells, which may explain the sustained antibody production in AIDS patients. Traditional end-point assays to detect anti-MA antibodies showed an unacceptable number of false positive and negative test results. Here a much improved biosensor method (the MARTI-assay, i.e. Mycolic acid Antibody Real-Time Inhibition assay) was developed to detect antibodies to mycolic acid in patient sera as surrogate markers of active tuberculosis. The test was assessed on an IAsys optical biosensor and gave an accuracy of 82%. The technology was transferred to an SPR (ESPRIT) biosensor to economise and simplify the assay. Mycolic acid containing liposomes were immobilized on the SPR gold surface pre-coated with octadecanethiol. The following parameters were optimized on the ESPRIT biosensor to enable reliable TB diagnosis: effect of degassed buffer, saponin blocking, first exposure to serum at low concentration and second exposure to antigen inhibited serum at high concentration. The IAsys biosensor system has a weakness in the double channel cuvette system, in which the channels often do not give matching results, while being ten times more expensive than the gold discs provided for the ESPRIT biosensor. The ESPRIT biosensor is provided with an adjustable laser setting to compensate for differences in the channel readings as well as an automated fluidic system that reduces variance from one sample to the next. First indications are that the test can also be used for prognosis of TB during treatment. It is hoped that the ESPRIT biosensor will improve the accuracy of the test to more than 90%. If the MARTI-assay technology could be made amenable for high throughput screening, it may provide the solution to the serodiagnosis of tuberculosis and monitoring of progress during TB treatment both in adult and children, thereby reducing the spread of TB within the communities.
CHANGES IN ASPHALTS (1984)

A review of recently published literature indicates that changes in asphalts are of considerable concern to those responsible for designing, constructing, and managing pavements. Data indicate that the ranges of properties of asphalts have not changed significantly, but recent world petroleum supply and economic climate may have caused more rapid changes in asphalt from certain sources. New refining or manufacturing techniques may also affect asphalt properties, but these causes probably account for a smaller portion of reported changes than variations in feedstocks that are primarily petroleum crude oils. There appears to have been a statistically significant but small change to a population of more temperature-susceptible asphalts, which may have been a result of specification changes and greater use of high smoke point asphalts (that may generally have higher temperature susceptibilities) with the use of increased mixing temperatures in drum mixers. Preferred methods of measuring low temperature performance are through the use of low temperature consistency measurements. Chemical analyses reported to date have not correlated well with performance because of the complex nature of asphalts. The changes in asphalt properties with use of antistripping additives is discussed and some factors affecting paving construction and pavement performance, which may be perceived as changes in asphalts, are listed.
Effects of NMDA receptor channel blockers, MK-801 and memantine, on locomotor activity and tolerance to delay of reward in Wistar−Kyoto and spontaneously hypertensive rats (2004)

N-Methyl-D-aspartate (NMDA) receptor blockade enhances motor activity and stimulates dopamine metabolism, effects shared with classical psychostimulant drugs. The present study aimed to characterize behavioral effects of two NMDA receptor channel blockers, MK-801 and memantine, in both Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats. In Experiment 1, SHR rats demonstrated higher spontaneous locomotor activity and spent more time in the central area of the open field apparatus compared with WKY rats. Rats of both strains pre-treated with MK-801 (0.01-0.3 mg/kg) or memantine (1-32 mg/kg) demonstrated dose-dependent increases in the total distance traveled and time spent in the central area. Experiment 2 was based on the two-lever discrete-trial delayed reinforcement task in which rats could press one lever to obtain one pellet immediately or another lever for four pellets delivered after a variable delay (0-60 s). Tolerance to delay of reward did not differ between strains. MK-801 (0.03-0.3 mg/kg) and memantine (1-10 mg/kg) produced small, but significant, facilitation of the large-reward lever responding and markedly impaired operant performance at higher dose levels (increased number of missed trials). For both experiments, effects of MK-801 and memantine were more pronounced in WKY compared with SHR rats. Additional studies are needed to address the utility of noncompetitive NMDA receptor blockers in the treatment of attention deficit and hyperactivity disorder.
Classifying texts using relevancy signatures (1992)

Text processing for complex domains such as terrorism is complicated by the difficulty of being able to reliably distinguish relevant and irrelevant texts. We have discovered a simple and effective filter, the Relevancy Signatures Algorithm, and demonstrated its performance in the domain of terrorist event descriptions. The Relevancy Signatures Algorithm is based on the natural language processing technique of selective concept extraction, and relies on text representations that reflect predictable patterns of linguistic context. This paper describes text classification experiments conducted in the domain of terrorism using the MUC-3 text corpus. A customized dictionary of about 6,000 words provides the lexical knowledge base needed to discriminate relevant texts, and the CIRCUS sentence analyzer generates relevancy signatures as an effortless side-effect of its normal sentence analysis. Although we suspect that the training base available to us from the MUC-3 corpus may not be large enough to provide optimal training, we were nevertheless able to attain relevancy discriminations for significant levels of recall (ranging from 11% to 47%) with 100% precision in half of our test runs.
Reliable and Efficient Way to Broadcast Messages in a Group by Trust-Based Broadcast (TBB) Scheme (2012)

Nowadays information systems are being shifted to distributed architectures, i.e. Grid and Peer-to-peer (P2P) models to obtain the benefits like scalability, autonomy, and fault-tolerance. We consider the P2P model as a fully distributed, scalable system, which is composed of peer processes (peers). Here, a group of multiple peers cooperate with each other. Peers have to efficiently and flexibly deliver messages to every peer of the group in P2P overlay networks. In order to efficiently and reliably broadcast messages in a scalable group, we take advantage of the multipoint relaying (MPR) mechanism. Here, each peer sends messages to only a subset of its acquaintances. However, if a peer which forwards messages to other peers is faulty, the peers cannot receive messages. In this paper, we newly discuss a trustworthiness-based broadcast (TBB) algorithm where only trustworthy peers forward messages. That is, untrustworthy peers are peers which cannot forward the messages due to some faults. Here, the transmission fault implied by faults of untrustworthy peers can be reduced. We evaluate the TBB algorithm in terms of the number of messages transmitted.
Truncation effects in Viterbi decoding (2003)

Practical Viterbi decoders often fall significantly short of full maximum likelihood decoding performance because of survivor truncation effects. In the present work the authors study the tradeoff between truncation length and performance loss for the two most common variations of Viterbi's algorithm: best-state decoding (BSD) and fixed-state decoding (FSD). It is found that FSD survivors should be about twice as long as BSD survivors for comparable performance.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Adaptive driver-automation shared steering control via forearm surface electromyography measurement (2020)

Shared steering control has been developed to reduce driver workload while keeping the driver in the control loop. A driver could integrate visual sensory information from the road ahead and haptic sensory information from the steering wheel to achieve better driving performance. Previous studies suggest that, compared with adaptive automation authority, fixed automation authority is not always appropriate with respect to human factors. This paper focuses on designing an adaptive shared steering control system via sEMG (surface electromyography) measurement from the forearm of the driver, and evaluates the effect of the system on driver behavior during a double lane change task. The shared steering control was achieved through a haptic guidance system which provided active assistance torque on the steering wheel. Ten subjects participated in a high-fidelity driving simulator experiment. Two types of adaptive algorithms were investigated: haptic guidance decreases when driver grip strength increases (HG-Decrease), and haptic guidance increases when driver grip strength increases (HG-Increase). These two algorithms were compared to manual driving and two levels of fixed authority haptic guidance, for a total of five experimental conditions. Evaluation of the driving systems was based on two sets of dependent variables: objective measures of driver behavior and subjective measures of driver workload. The results indicate that the adaptive authority of HG-Decrease yielded lower driver workload and reduced the lane departure risk compared to manual driving and fixed authority haptic guidance.
Microflow Technology in Polymer Synthesis (2012)

Microflow technology, i.e., the use of microfluidic devices for continuous flow synthesis, represents a highly useful and increasingly popular method in organic chemistry. Recently, also an increasing number of polymer synthesis protocols attain benefit from this technique. In particular, the control of highly exothermic, fast polymerization reactions can be improved due to the excellent heat and mass transfer within the small dimensions of the microreactors. Continuous flow setups with different micromixer geometries and flow patterns are currently used for the preparation of a variety of macromolecular architectures by ionic and (controlled) radical polymerization techniques. This Perspectives reviews recent developments in synthetic strategies and reactor design for the homogeneous synthesis of polymers in microflow systems and emphasizes future challenges and promise for applications. Polymer synthesis by radical, anionic, cationic, and coordinative polymerization is considered as well as different polymer topologies generated (linear, branched, and dendritic architectures).
Are Barrier Crashes Sufficient for Evaluating Air Bag Sensor Performance? (1990)

<div class="htmlview paragraph">This is the fifth in a series of papers on the theory of sensing automobile crashes [<span class="xref">1</span>, <span class="xref">2</span>, <span class="xref">3</span> and <span class="xref">4</span>]. The focus of this paper is to analyze why barrier crash tests are presently conducted and to propose a methodology for determining what crash tests should be run for better overall performance.</div>
On vocabulary-independent speech modeling (2002)

The use of vocabulary-independent (VI) models to improve the usability of speech recognizers is described. Initial results using generalized triphones as VI models show that with more training data and more detailed modeling, the error rate of VI models can be reduced substantially. For example, the error rates for VI models with 5000, 10000, and 15000 training sentences, are 23.9%, 15.2%, and 13.3%, respectively. Moreover, if task-specific training data are available, one can interpolate them with VI models. This task adaptation can reduce the error rate by 18% over task-specifying models.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Modelling the dynamic drape of fabrics on synthetic humans: a physical, lumped‐parameter model (1995)

True 3‐D garment design (CAD) systems are fundamental for the next generation of intelligent textile and garment manufacture and retailing. Reports a new approach for modelling fabric. The fabric model is developed based on a physical analogue to a deep shell system for describing and predicting the real 3‐D shape of clothes. The fabric motion is determined by deformation energy, gravity and external constraints, such as collision forces, using the deformable node bar concept. The advantages of this model are that engineering parameters can be used as model parameters directly and that the model is configured based on the surface co‐ordinate system, which is believed to be important as the basis of a powerful fashion CAD system. The model successfully simulated fabric drape and has been implemented on a synthetic female model.
Soft Cardinality in Semantic Text Processing: Experience of the SemEval International Competitions (2015)

Soft cardinality is a generalization of the classic set cardinality (i.e., the number of elements in a set), which exploits similarities between elements to provide a counting of the number of elements in a collection. This model is so general that can be used interchangeability as cardinality function in resemblance coefficients such as Jaccard's, Dice's, cosine and others. Beyond that, cardinality-based features can be extracted from pairs of objects being compared to learn adaptive similarity functions from training data. This approach can be used for comparing any object that can be represented as a set or bag. We and other international teams used soft cardinality to address a series of natural language processing (NLP) tasks in the recent SemEval (semantic evaluation) competitions from 2012 to 2014. The systems based on soft cardinality have always been among the best systems in all the tasks in which they participated. This paper describes our experience in that journey by presenting the generalities of the model and some practical techniques for using soft cardinality for NLP problems.