gebhart/patrepeval huggingface dataset:
Detection of Cell Wall Galactomannoprotein Afmp1p in Culture Supernatants of <i>Aspergillus fumigatus</i> and in Sera of Aspergillosis Patients (2002)

Mannoproteins are important and abundant structural components of fungal cell walls. The AFMP1 gene encodes a cell wall galactomannoprotein of Aspergillus fumigatus. In the present study, we show that Afmp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Afmp1p was capable of detecting this protein from the cell culture supernatant of A. fumigatus. The anti-Afmp1p antibody is specific since it fails to react with any protein from lysates of Aspergillus flavus, Aspergillus niger, Aspergillus terreus, Penicillium marneffei, Candida albicans, Cryptococcus neoformans, Blastomyces dermatitidis, and Histoplasma capsulatum by Western blotting. In addition, this Afmp1p antigen-based ELISA is also specific for A. fumigatus since the cell culture supernatants of the other eight fungi gave negative results. Finally, a clinical evaluation of sera from invasive aspergillosis patients indicates that 8 of 15 (53%) patients are Afmp1p antigen test positive. Furthermore, an Afmp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for invasive aspergillosis carry a sensitivity of 86.7% (13 of 15). The specificities of the tests are high since none of the 138 control sera, including 100 from normal blood donors, 20 from patients with penicilliosis marneffei, 6 from patients with candidemia, 8 from patients with typhoid fever, and 4 from patients with melioidosis, was positive by either test. In conclusion, the combined Afmp1p antibody and antigen tests are highly sensitive and specific for A. fumigatus invasive aspergillosis.
User-Friendly Graph Editing for Procedural Modeling of Buildings (2010)

A proposed rule-based editing metaphor intuitively lets artists create buildings without changing their workflow. It's based on the realization that the rule base represents a directed acyclic graph and on a shift in the development paradigm from product-based to rule-based representations. Users can visually add or edit rules, connect them to control the workflow, and easily create commands that expand the artist's toolbox (for example, Boolean operations or local controlling operators). This approach opens new possibilities, from model verification to model editing through graph rewriting.
Separation of the bacterial species, Escherichia coli, from mixed-species microbial communities for transcriptome analysis (2011)

A method based on immuno-magnetic separation (IMS) and application of RNAlater was developed to separate a bacterial species, E. coli as an example, from mixed-species communities while preserving its transcriptome. The method combined with cDNA microarray analysis should be very useful to study species interactions in mixed-species communities.
Transition between Collective Behaviors of Micromotors in Response to Different Stimuli (2013)

We report a Ag(3)PO(4) microparticle system showing collective behaviors in aqueous medium. Transition between two emergent patterns, namely "exclusion" and "schooling", can be triggered by shift in chemical equilibrium upon the addition or removal of NH(3) or in response to UV light. The transitions are consistent with a self-diffusiophoresis mechanism resulting from ion gradients. The reported system is among the few examples of nanomotors that are based on a reversible nonredox reaction and demonstrates new design principles for micro/nanomotors. Potential applications of the reported system in logic gates, microscale pumping, and hierarchical assembly have been demonstrated.
In Situ 119Sn Conversion Electron Mössbauer Study of the Surface of Tin Layers as Deposited by an Electroless Process (1986)

In situ119Sn conversion electron Mössbauer spectroscopy has been used to investigate the process of autocatalytic (electroless) tin deposition in a strong alkaline solution using a rotating disk. It is very likely that a tin(II) compound—probably some tin(II) hydroxide complex—is present at the surface during the Sn‐metal depositing disproportionation reaction. The results obtained in a tin(II) solution with a pH of 6.1 indicate the presence at the surface of what is probably a tin(II) chloride compound.
Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral stationary phase (1998)

The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed-phase mode, the normal-phase mode, or the polar-organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase to another.
pH, Temperature and Lactate Production in Human Red Blood Cells: Implications for Blood Storage and Glycolytic Control (1992)

The interaction of temperature and pH in biological systems comprises two components. Temperature change may perturb the pH of solutions, and it may change the pKa of some ionizable groups that are involved in enzyme catalysis. The pH optima of single reactions and whole pathways are therefore temperature sensitive. The pH optimum of glycolysis in human red cells has been investigated only at 37 °C. We have measured the effect of temperature on the pH of stored blood suspensions and on the pH optimum of glycolysis in the human red cell. The pH of the cell suspensions in a traditional storage medium was 7.25 ± 0.2 at 4°C. The pH optimum of glycolysis was high (7.8-8.5) between 15 and 35 °C. It can be inferred from our data that human red cells are currently stored at least 0.5 pH units below the pH optimum of glycolysis at 4°C. This suggestion is supported by storage experiments which showed that glycolysis at 4°C was at least 1.5-fold more active at an initial pH of 7.67 versus 7.36. Equations which describe the variation in reaction velocity with pH were fitted to the pH curves for glycolysis in order to identify the ionizable groups that contribute to the effect of pH on glycolysis. It is generally accepted that hexokinase catalyses the rate-limiting step in glycolysis in the human red cell, but none of the ionizable groups implicated correspond to that involved in the hexokinase reaction.
Preparation of Metallic Calcium by Electrolysis of Calcium Oxide Dissolved in Molten Calcium Chloride (1964)

Metallic calcium was prepared by electrolysis of calcium oxide dissolved in molten calcium chloride in a modified moving cathode‐type cell. For comparison, control electrolyses of the chloride were made. Average cell voltage with the oxide‐bearing electrolyte ranged up to 23% less than that with the chloride alone. The oxide content of the electrolyte decreased due to electrolysis, and tests showed that chlorine was not released at the anode. This appears to be the first demonstration that calcium oxide can be fed to a chloride electrolyte for calcium production.
Coaxial Radio Frequency (RF) Diathermy in Anterior Segment Surgery (1985)

Modern ophthalmic microsurgery, particularly cataract extraction, trabeculectomy, and other forms of glaucoma filtration surgery, has been characterized by ever increasing precision. The use of radio frequency (RF) currents for cautery of blood vessels in anterior segment microsurgery has several advantages over other methods widely employed, including the bipolar cautery. A coaxial monopolar underwater diathermy probe, which operates with RF current has been specifically developed for anterior segment microsurgery and has proved vastly superior in cataract surgery, trabeculectomy, and full thickness glaucoma filtration procedures.
Saving and Uncertainty: The Precautionary Demand for Saving (1968)

I. Introduction, 465. — II. A two period model of consumption, 466. — III. The effect of uncertainty on saving, 467. — IV. Decreasing risk aversion and precautionary saving, 468. — V. Speculations on the consumption function, 470. — VI. Conclusion, 472.
Magnetic properties of a new 3D-cobalt phosphonate: Co3(O3PC2H4CO2)2 (1999)

The magnetic properties of a new cobalt(II) phosphonate have been investigated. The compound is isostructural with the pillared layered metal phosphonates M 3 (O 3 PC 2 H 4 CO 2 ) 2 , M=Zn, Mn. In this structure, the magnetic transition metal ions are arranged within layers formed by the interconnection of CoO 4 tetrahedra and CoO 6 octahedra. The ac and dc magnetic susceptibilities and magnetization measurements show that Co 3 (O 3 PC 2 H 4 CO 2 ) 2 is a canted antiferromagnet at T N =15.5 K. Below T N , the hysteresis cycles exhibit an S shape related to a spin flop effect. The specific heat measurements indicate that large short-range correlations are present above T N in relation with the layered character of the structure.
Robust Multi-body Feature Tracker: A Segmentation-free Approach (2016)

Feature tracking is a fundamental problem in computer vision, with applications in many computer vision tasks, such as visual SLAM and action recognition. This paper introduces a novel multi-body feature tracker that exploits a multi-body rigidity assumption to improve tracking robustness under a general perspective camera model. A conventional approach to addressing this problem would consist of alternating between solving two subtasks: motion segmentation and feature tracking under rigidity constraints for each segment. This approach, however, requires knowing the number of motions, as well as assigning points to motion groups, which is typically sensitive to the motion estimates. By contrast, here, we introduce a segmentation-free solution to multi-body feature tracking that bypasses the motion assignment step and reduces to solving a series of subproblems with closed-form solutions. Our experiments demonstrate the benefits of our approach in terms of tracking accuracy and robustness to noise.
Novel detection and differential utilization of a c-myc transcriptional block in colon cancer chemoprevention. (2002)

Mutations in the adenomatous polyposis coli (APC) gene, which initiate almost all human colon cancers, directly target the proto-oncogene, c-myc, by elevating beta-catenin/T-cell factor (TCF) signaling. We have shown that agents ascribed chemopreventive activity for colon cancer in fact also stimulate beta-catenin/TCF activity in vitro. Their effects on c-myc transcription were assayed using a novel variant of fluorescence in situ hybridization that detects c-myc transcription sites in intact nuclei. Increased transcriptional initiation of c-myc induced by the short-chain fatty acid, butyrate, consistent with elevated beta-catenin/TCF activity, was efficiently abrogated by a block to transcriptional elongation, resulting in decreased c-myc expression. 1alpha,25-Dihydroxyvitamin D(3) also induced transcriptional blockage. In contrast, the nonsteroidal anti-inflammatory drug, sulindac, increased c-myc expression, an effect attributable at least in part to its failure to induce transcriptional blockage. We have described a novel approach for evaluating the effects of chemopreventive agents on the expression of a gene critical in colonic tumorigenesis.
Purification and characterization of a protein that binds to the recombination signal sequence of the immunoglobulin J<sub>x</sub>segment (1989)

A protein that binds to the recombination signal sequence (RS) of the immunoglobulin J kappa segment was purified almost to homogeneity from the nuclear extract of a murine pre-B cell line 38B9. A similar binding protein was found in lymphoid cell lines but not in non-lymphoid cell lines. The binding activity was associated with a polypeptide with a molecular weight of 60,000. DNase I footprinting analysis demonstrated that this binding protein interacted with the heptamer and several 3' bases close to the heptamer. The Kd value of the J kappa RS binding protein to the J kappa RS was 1 nM. One base substitution in the heptamer of the J kappa RS greatly reduced the affinity of the J kappa RS binding protein. The high specificity of the binding site of the J kappa RS binding protein suggests that this protein may be involved in V-J recombination.
Isolation, Structural Assignment, and Synthesis of <i>N</i>-(2-Methyl-3-oxodecanoyl)-2-pyrroline, a New Natural Product from <i>Penicillium </i><i>b</i><i>revicompactum</i> with in Vivo Anti-Juvenile Hormone Activity (1998)

A new natural product with in vivo anti-juvenile hormone (JH) activity, N-(2-methyl-3-oxodecanoyl)-2-pyrroline (2), has been isolated from Penicillium brevicompactum Dierckx. Its structure has been tentatively assigned based on spectral data and unambiguously confirmed by alternative syntheses. Compound 2 has been prepared by means of a sequence of reactions beginning with acylation of Meldrum's acid by octanoyl chloride. The subsequent steps have been aminolysis of the resultant intermediate with pyrrolidine, alkylation of the active position with iodomethane, introduction of a methoxy group in the pyrrolidine ring by anodic oxidation, and final elimination of methanol through adsorption on SiO2 and heating. The natural product 2 and its bicyclic isomer 2-heptyl-3-methyl-4-oxo-6,7,8,8a-tetrahydro-4H-pyrrolo[2,1-b]-1,3-oxazine (3) are also obtained. This reveals that 2 can be biogenetically related to the recently discovered brevioxime (1). Compound 2 and the synthetic precursors of 2 have shown important biological activities as insecticides (against Oncopeltus fasciatus Dallas) and fungicides. The natural product induces precocious metamorphosis in the target insect (70% precocious adults at 10 μg/nymph). In view of the above results, these products could be useful as lead molecules for the synthesis of analogues with enhanced biological activities.
Fault Tree-Based Reliability Assessment of a 132-kV Transmission Line Protection Scheme (2013)

The reliability of a power system network depends greatly on the performance of the protection system. Improving the reliability of the protection scheme of the transmission lines will enhance the overall reliability of the power system network. The focus of this paper is the improvement of the reliability of a power system transmission line using fault tree analysis (FTA). The paper considers the development of fault tree diagrams for the protection scheme of the 150km-long 132-kV transmission line in Northern Nigeria. The existing protection scheme is analyzed and compared with a proposed scheme equipped with a redundancy arrangement. And the result shows that the new scheme offers significant improvement (about 51%) in the availability of the line.
Heterosis and Combining Ability in Hybrids Derived from Oilseed Rape Cultivars and Inbred Lines (1989)

Basic genetic information needed to develop hybrid oilseed rape ( Brassica napus spp. oleifera ) cultivars has not been previously reported. Since recent work had shown oilseed rape cultivars to be genetically heterogeneous, inbred line derived hybrids were compared to cultivar derived hybrids to determine if there were differences among inbred lines in terms of their performance in hybrid combination. For inbred line derived hybrids, midparent was compared to general combining ability as a means of predicting hybrid yield. Three inbred lines, from each of three source cultivars, were crossed using a factorial mating design and the resultant hybrids were field evaluated in three environments. The inbred lines, source cultivars, and cultivar hybrids were included as checks. Yields of inbred line derived hybrids were compared to their respective cultivar derived hybrids and some inbred line crosses were found to be significantly ( P < 0.05) higher and others significantly ( P < 0.05) lower yielding. There were also differences in general combining ability (GCA) effects among inbred lines. This result indicates the presence of variability in breeding values among cultivar derived inbred lines and that hybrid oilseed rape breeding programs should be based on inbred line crosses, rather than cultivar crosses. General combining ability (GCA) was significant ( P < 0.01) and accounted for 88% of the cross sums of squares, while specific combining ability (SCA) was nonsignificant ( P > 0.05) indicating that additive genetic effects predominantly influence the expression of yield. Regression of midparent on observed hybrid yield was significant ( P < 0.01), with an r 2 of 0.37. Regression of expected hybrid yield, based on parental GCA, on observed hybrid yield was also significant ( P < 0.01), but with an r 2 of 0.88. Therefore, GCA was more effective than midparent for describing hybrid yield. There was, however, a positive relationship between midparent and hybrid yield indicating that despite the somewhat low r 2 , midparent may still be a useful guide for the selection of parents for testing in hybrid combination.
Studies of inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by using a visible femtosecond laser: insight into the possible inactivation mechanisms (2011)

We report experimental results on the inactivation of encephalomyocarditis virus, M13 bacteriophage, and Salmonella typhimurium by a visible femtosecond laser. Our results suggest that inactivation of virus and bacterium by a visible femtosecond laser involves completely different mechanisms. Inactivation of viruses by a visible femtosecond laser involves the breaking of hydrogen/hydrophobic bonds or the separation of the weak protein links in the protein shell of a viral particle. In contrast, inactivation of bacteria is related to the damage of their DNAs due to irradiation of a visible femtosecond laser. Possible mechanisms for the inactivation of viruses and bacteria are discussed.
Molecular methods for bacterial genotyping and analyzed gene regions (2011)

Bacterial strain typing is an important process for diagnosis, treatment and epidemiological investigations. Current bacterial strain typing methods may be classified into two main categories: phenotyping and genotyping. Phenotypic characters are the reflection of genetic contents. Genotyping, which refers discrimination of bacterial strains based on their genetic content, has recently become widely used for bacterial strain typing. The methods already used in genotyping of bacteria are quite different from each other. In this review we tried to summarize the basic principles of DNA-based methods used in genotyping of bacteria and describe some important DNA regions that are used in genotyping of bacteria. J Microbiol Infect Dis 2011;1(1):42-46. doi: 10.5799/ahinjs.02.2011.01.0007
Simulation of Combustion Field by Lattice Boltzmann Method. (2002)

For modeling turbulent combustion, numerous studies have been made. Even chemically nonreacting turbulent flows are complex phenomena, and chemical reactions make the problem more complicated. Due to the limitation of computational costs, conventional numerical methods are impractical in carrying out 3 D numerical simulations at high Reynolds numbers with detailed chemistry. The lattice Boltzmann method (LBM) has emerged as an efficient alternative for numerical simulation of complex flows. In our recent study, we have proposed a numerical scheme to simulate combustion field by LBM. Here, for benchmark study, we focus on a flame in simple flow geometry. The numerical model and LB equations for flow, temperature, and concentration are explained in detail. Using methane/air and propane/air mixtures, we obtain laminar burning velocities, which are compared with experimental values.
Paleo seismic and color acoustic impedance sections: Applications of downward continuation in structural and stratigraphic context (1982)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1982Paleo seismic and color acoustic impedance sections: Applications of downward continuation in structural and stratigraphic contextAuthors: M. Turhan TanerErnest E. CookNorman S. NeidellM. Turhan TanerSeismic Research Corp., Ernest E. CookZenith Exploration Co., Inc., and Norman S. NeidellZenith Exploration Co., Inc.https://doi.org/10.1190/1.1826837 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1826837FiguresReferencesRelatedDetailsCited by4. Migration21 March 20128. Earth Imaging in Depth21 March 2012 SEG Technical Program Expanded Abstracts 1982ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1982 Pages: 520 publication data© 1982 Copyright © 1982 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished Online: 24 Mar 2005 CITATION INFORMATION M. Turhan Taner, Ernest E. Cook, and Norman S. Neidell, (1982), "Paleo seismic and color acoustic impedance sections: Applications of downward continuation in structural and stratigraphic context," SEG Technical Program Expanded Abstracts : 110-111. https://doi.org/10.1190/1.1826837 Plain-Language Summary PDF DownloadLoading ...
Sabot Design Optimization (1981)

SABOT discard aerodynamics are described using local shock/expansion procedures. Optimization was based upon a figure of merit for pitching moment impulse, precluding sabot impact on the projectile stabilizing fins. Optimum designs exhibit initial lateral motion with small rotation minimizing mechanical moments and facilitating fin clearance, followed by rapid pitch-up/deceleration minimizing interaction duration.
Dynamic Yield Strengths of B4C, BeO, and Al2O3 Ceramics (1971)

The dynamic yield strengths for several ceramics were measured under shock-wave compression. The Hugoniot elastic limit (HEL), taken as the amplitude of the observed elastic precursor traveling ahead of the compressional wave, was 154 and 82 kbar for B4C and BeO ceramics, respectively, and ranged from 61 to 134 kbar for several Al2O3 ceramics. These values apply to 6-mm-thick samples; in some cases there were variations with sample thickness. The offset of the Hugoniot from the isotropic compression curve in the stress-volume plane indicates a nonzero yield strength above the HEL for Al2O3. This yield strength is somewhat less than the yield strength at the HEL. The compaction of porous ceramics is found to follow a quadratic variation with stress, full compaction being reached at about four times the HEL.
Electronic properties of low-temperature grown III/V thin films (1994)

Abstract : Our study of LT GaAs in the previous contract is furthered by: (1) employing several novel techniques to investigate the defects present and the transport mechanism in LT GaAs. (2) expanding to other LT-III/V compounds and (3) exploring the unique properties of these LT semiconductors for device applications. Photocurrent spectroscopy of annealed LT GaAs gives clear evidence of substantial presence of As(sub Ga) defects in the material. The 'internal photoemission' spectrum is actually found to be due to photoexcitation from As(sub Ga) defects. Magnetic circular dichroism and near-infrared absorption provide sensitive and quantitative measurements of the concentration of the As(sub Ga) defects in both neutral and positively charged states. A systematical study of materials grown and annealed at different temperatures shows that As(sub Ga) defects can account for the pinning of Fermi energy and play a dominant role in the materials. Nuclear magnetic resonance has started to be used to study LT GaAs. Although the study is in its early stage, it has been demonstrated that by using a spin echo technique it is possible to obtain characteristic responses from As in perfect sites and defective sites. in contrast to LT GaAs. LT lnP was found to be highly conductive. jg