gebhart/patrepeval huggingface dataset:
Identification of protective components of two major outer membrane proteins of spotted fever group Rickettsiae. (2001)

Fragments representing the genes of the two major outer membrane proteins of spotted fever group rickettsiae (rOmpA and rOmpB) were tested as DNA vaccines. Immunizations with each of three fragments (rompA4999-6710, rompB1550-2738, and rompB2459-4123) conferred a degree of protection on vaccinated mice against virulent rickettsial challenge. Protection was achieved when DNA immunizations were followed by booster immunizations with the homologous recombinant protein. Proliferation and gamma-interferon secretion were detected after in vitro stimulation of lymphocytes from immunized animals with whole Rickettsia conorii antigen. The data validate particular segments of rOmpA and rOmpB as potent immunogens and hence as sources of immunostimulatory elements with specificity for T lymphocytes, which are the key effectors of protective immunity against rickettsial infections.
Programming the InfiniBand Network Architecture for High Performance Message Passing Systems. (2003)

The InfiniBand Architecture provides availability, reliability, scalability, and performance for server I/O and inter-server communication. The InfiniBand specification not only describes the physical interconnect and the highlevel management functions but also a wide range of functions from simple unreliable communication to network partitioning, with many options. The specification defines line protocols for message passing as a collection of proto verbs. This paper discusses various programming options that available to benefit from the capabilities of InfiniBand Network Architecture. An analysis is presented of the various strategies and programming models that would be enabled and/or enhanced to benefit from the InfiniBand infrastructure, for message passing systems. Conclusions are offered about benefits, trends, and further opportunities for low-level InfiniBand programming.
Role of Protein and Lactose Interactions in the Age Gelation of Ultra-High Temperature Processed Concentrated Skim Milk (1993)

Skim milk was pasteurized, diafiltered, and concentrated three times by UF. Lactose or sucrose was then added at 3 or 6%. The five samples containing < .05% lactose, 3 and 6% lactose, and 3 and 6% sucrose were UHT processed at 140 degrees C for 4 s using indirect heating, collected aseptically in presterilized containers, and stored at 4, 20, and 35 degrees C. All samples stored at 4 and 20 degrees C gelled after 21 wk of storage. Samples stored at 35 degrees C did not gel. Browning occurred only in samples containing lactose stored at 35 degrees C. Proteolysis in gelled samples was shown by SDS-PAGE. Bands were due to proteolysis, protein crosslinking, and a streaking pattern in ungelled samples. Electron micrographs of gelled samples showed that various casein particles were connected by hairlike protrusions, but the micelles in ungelled samples were not connected and had few protrusions. The Maillard reaction neither promoted nor deferred age gelation. Protein modifications prevented gelation in samples stored at 35 degrees C. Age gelation was probably a two-step process in which dissociated proteins from the casein micelles reformed on micelles as hairlike protrusions. This process was followed by aggregation of the protein particles.
Crystal chemistry of portland cement hydrates as radioactive waste hosts. Final report, June 15, 1983-June 14, 1984. [Fixation of iodine by calcium aluminate hydrates, and fixation of strontium and cesium by using Stratling's compound] (1984)

Portland cement hydrates have been used as encapsulant/host phases in radioactive waste management. However, their phase chemistry and stability relationships are poorly defined. Therefore, on occasion, they have not performed as well as expected. As a result, their use has been mainly limited to low-level waste disposal. Since this knowledge gap existed, we had begun to investigate the crystal chemistry of the portland cement hydrates. It was our objective to identify potential hydrate host phases which were not only suitable for isolating radioactive-waste species but also inexpensive, easily processed, low-temperature materials. Initially, we were concentrating upon two areas of interest: the fixation of iodine by the calcium aluminate hydrates and the feasibility of using Stratling's compound as a host phase for cesium and strontium fixation. In both cases, a phase equilibrium study was initiated in order to identify phase relations and consequences of adding the species of interest to the system. An iodine-containing analogue of calcium monosulfoaluminate hydrate (C/sub 3/A.CaI/sub 2/.xH/sub 2/O) was identified as a possible host phase. CsOH was added to formulations in the neighborhood of Stratling's compound, in order to establish phase relations and identify the fixation ability of Stratling's compound and its associated hydrates. 11 figures,more » 9 tables.« less
Ataxin-3 With an Altered Conformation That Exposes the Polyglutamine Domain Is Associated With the Nuclear Matrix (1999)

Spinocerebellar ataxia type-3 or Machado-Joseph disease (SCA3/MJD) is a member of the CAG/polyglutamine repeat disease family. In this family of disorders, a normally polymorphic CAG repeat becomes expanded, resulting in expression of an expanded polyglutamine domain in the disease gene product. Experimental models of polyglutamine disease implicate the nucleus in pathogenesis; however, the link between intranuclear expression of expanded polyglutamine and neuronal dysfunction remains unclear. Here we demonstrate that ataxin-3, the disease protein in SCA3/MJD, adopts a unique conformation when expressed within the nucleus of transfected cells. The monoclonal antibody 1C2 is known preferentially to bind expanded polyglutamine, but we find that it also binds a fragment of ataxin-3 containing a normal glutamine repeat. In addition, expression of ataxin-3 within the nucleus exposes the glutamine domain of the full-length non-pathological protein, allowing it to bind the monoclonal antibody 1C2. Fractionation and immunochemical experiments indicate that this novel conformation of intranuclear ataxin-3 is not due to proteolysis, suggesting instead that association with nuclear protein(s) alters the structure of full-length ataxin-3 which exposes the polyglutamine domain. This conformationally altered ataxin-3 is bound to the nuclear matrix. The pathological form of ataxin-3 with an expanded polyglutamine domain also associates with the nuclear matrix. These data suggest that an early event in the pathogenesis of SCA3/MJD may be an altered conformation of ataxin-3 within the nucleus that exposes the polyglutamine domain.
Binding of hirudin to meizothrombin (1998)

Prothrombin (coagulation factor II) is the inactive precursor molecule of thrombin (coagulation factor IIa). Proteolytic cleavage of the peptide bond Arg320-Ile321 converts prothrombin into the two-chain thrombin precursor meizothrombin. Meizothrombin hydrolyses peptidyl substrates, but cleavage of fibrinogen is poor. Unfortunately, meizothrombin exhibits a significant autocatalytic activity and thus is not structurally stable in solution. Hirudin, the 65-residue peptide anticoagulant from the salivary gland of the European leech Hirudo medicinalis, is a highly specific and effective thrombin inhibitor. To study the interactions of meizothrombin and hirudin, recombinant prothrombin with active site Asp419 replaced by Asn (D419N-prothrombin) was produced in CHO cells and transformed into D419N-meizothrombin in vitro. D419N-meizothrombin exhibited no proteolytic and autocatalytic activity. D419N-meizothrombin was affinity purified at an immobilized C-terminal hirudin-derived peptide demonstrating the presence and activity of the anion binding exosite. D419N-meizothrombin exhibited binding activity to hirudin immobilized at the solid phase in an ELISA. Incubation of D419N-meizothrombin with hirudin resulted in a significant increase of intrinsic fluorescence. Fluorescence titration of D419N-meizothrombin with hirudin produced a sharp break in the titration curve at the molar equivalence point and a total fluorescence enhancement of 24%. However, the titration curve did not reflect a simple binding mechanism. Incubation of D419N-meizothrombin with fibrinopeptide A and C-terminal hirudin peptide 54-65 did not change fluorescence emission. Trp468 located in the gamma-loop of thrombin was replaced by Phe in the double-mutant D419N/W468F-thrombin. Similar to D419N-thrombin and D419N-meizothrombin, formation of the D419N/W468F-thrombin/hirudin complex resulted a significant increase in intrinsic fluorescence. Apparently, the binding of hirudin induces similar structural changes in both meizothrombin and thrombin. The structural change does not involve the flexible gamma-loop. The results suggest that meizothrombin binds hirudin similar to thrombin.
A Study on the Effect of the Department of Military NCO University students on Body composition Change in 15th week Exercise (2018)

본 연구의 목적은 15주간 운동이 군부사관과 대학생의 체성분 변화에 미치는 영향을 규명하는데 있다. 연구 대상은 경북 소재의 P대학교 군부사관과 남학생 4명, 여학생 10명을 대상으로 1회 100분 기준, 유산소 운동과 근력 및 근지구력 운동 중 자신이 선호하는 운동을 주3회 15주간 운동을 실시하여 체성분의 변화를 분석하였다. 체성분 분석은 ㈜자원메디컬사의 X-Scan Plus2를 이용하였고, 측정항목은 제지방, 근육량, 체수분량, 체지방량, 체지방률 5가지로 하였다. 자료 분석은 운동시작 1주차(운동프로그램 시작 전)에 5가지 항목을 측정하고 15주차 이후에 같은 항목을 재측정 한 후 운동의 효과성을 측정(분석)하기 위해 비모수 검정 방법의 한 형태인 Wilcoxon-test를 통해 5가지 항목의 사전과 사후를 비교 연구하였다. 연구한 결과 다음과 같은 결론을 얻었다. 15주 운동에 참여한 학생들은 운동 전후에 제지방, 근육량, 체수분량, 체지방량, 제지방률 등 모든 영역에 있어 변화가 있었지만, 체지방량의 변화만이 통계적으로 유의미한 것으로 나타났다.The purpose of this study was to investigate the effect of 15th week exercise on body composition of department of military NCO university students. The subjects of this study were P university department of military NCO in Gyeong-buk Province, 4 male students, and 10 female students were given 1 time per 100 minutes and subjected to exercise for 3 times a week for 15 consecutive weeks during the aerobic exercise, muscle strength and endurance exercise. The Body composition changes were analyzed. Body composition analysis was performed using X-Scan Plus2 manufactured by JAWON MEDICAL Co, The measurement items was fat, muscle mass, body water content, body fat mass, and percent body fat were 5 items. Data analysis was performed on 5 items in the first week of the exercise and the same items were re - measured in the 15th week. To assess the effectiveness of exercise, Wilcoxon-test, a form of nonparametric testing was performed. through measurement compared the pre- and post-tests of the 5 items. The results of the study were as follows. The students who participated in the 15th week Exercise showed changes in all areas including fat, muscle mass, body water, body fat and fat content before and after exercise, but only changes in body fat were statistically significant.
Discovery of Novel Small-Molecule Inhibitors of Human Epidermal Growth Factor Receptor-2: Combined Ligand and Target-Based Approach (2008)

Consensus virtual screening models were generated and validated utilizing a set of known human epidermal growth factor receptor-2 (HER2) inhibitors and modeled HER2 active and inactive state structures. The virtual screening models were successfully employed to discover a set of structurally diverse compounds with growth inhibitory activity against HER2-overexpressing SKBR3 breast cancer cell line. A search of a 3D database containing 350000 small-molecules using the consensus models retrieved 531 potential hits. Of the 531 hits, 57 were selected for testing in SKBR3 cells on the basis of structural novelty and desirable drug-like properties. Seven compounds inhibited growth of SKBR3 cells with IC50 values <10 microM. These lead compounds have desirable physicochemical properties and are excellent candidates for further optimization.
A wearable pre-impact fall early warning and protection system based on MEMS inertial sensor and GPRS communication (2015)

Fall is one of great threats affecting people with old age. Aimed at the falling issue of aged, the paper explored a pre-impact fall early warning and protection system. This system consists of an early fall alarm, protection airbags, a remote monitoring platform and a guardian's cellphone app. The early fall alarm and airbags are integrated in a belt, convenient for wearing and hip-protection. The inner of early fall alarm has a MEMS sensor, which collects 3-axis accelerated velocity and 3-axis angular velocity. A fall detection algorithm is applied to recognize falls from activities of daily living (ADL). When there is a dangerous movement approaching fall, the early fall alarm will warn the aged to stop the movement. When the fall happens, the early fall alarm will trigger the airbag system, then the airbags in the belt will inflate as soon as possible to reduce the damage to the aged. In addition, the early fall alarm will ring and send message to the guardian's cellphone for help. Meanwhile, the kinematics of the human body during falling time will be stored in TF card and sent to remote monitoring platform for storage. Then the monitoring platform can show the fall location where the fall incident happens in the electronic map. In order to test the reliability of this early fall alarm and protection system, a series of experiments have been designed. The results show that this system can be relatively accurate to detect falls, accomplishing functions including early fall warning and alarming, airbag inflation, statics transferring and storage, real-time location, which has significant benefit for reducing the direct damage and shortening the aiding time.
Angiotensin II Type 2 Receptor Stimulation of Neuronal Delayed-Rectifier Potassium Current Involves Phospholipase A<sub>2</sub>and Arachidonic Acid (1998)

Angiotensin II (Ang II) elicits an Ang II type 2 (AT2) receptor-mediated increase in delayed-rectifier K+ current (IK) in neurons cultured from newborn rat hypothalamus and brainstem. This effect involves a pertussis toxin (PTX)-sensitive Gi protein and is abolished by inhibition of serine and threonine phosphatase 2A (PP-2A). Here, we determined that Ang II stimulates [3H]arachidonic acid (AA) release from cultured neurons via AT2 receptors. This effect of Ang II was blocked by inhibition of phospholipase A2 (PLA2) and by PTX. Because AA and its metabolites are powerful modulators of neuronal K+ currents, we investigated the involvement of PLA2 and AA in the AT2 receptor-mediated stimulation of IK by Ang II. Single-cell reverse transcriptase (RT)-PCR analyses revealed the presence of PLA2 mRNA in neurons that responded to Ang II with an increase in IK. The stimulation of neuronal IK by Ang II was attenuated by selective inhibitors of PLA2 and was mimicked by application of AA to neurons. Inhibition of lipoxygenase (LO) enzymes significantly reduced both Ang II- and AA-stimulated IK, and the 12-LO metabolite of AA 12S-hydroxyeicosatetraenoic acid (12S-HETE) stimulated IK. These data indicate the involvement of a PLA2, AA, and LO metabolite intracellular pathway in the AT2 receptor-mediated stimulation of neuronal IK by Ang II. Furthermore, the demonstration that inhibition of PP-2A abolished the stimulatory effects of Ang II, AA, and 12S-HETE on neuronal IK but did not alter Ang II-stimulated [3H]-AA release suggests that PP-2A is a distal event in this pathway.
On the Value of Mitigation and Contingency Strategies for Managing Supply Chain Disruption Risks (2006)

We study a single-product setting in which a firm can source from two suppliers, one that is unreliable and another that is reliable but more expensive. Suppliers are capacity constrained, but the reliable supplier may possess volume flexibility. We prove that in the special case in which the reliable supplier has no flexibility and the unreliable supplier has infinite capacity, a risk-neutral firm will pursue a single disruption-management strategy: mitigation by carrying inventory, mitigation by single-sourcing from the reliable supplier, or passive acceptance. We find that a supplier’s percentage uptime and the nature of the disruptions (frequent but short versus rare but long) are key determinants of the optimal strategy. For a given percentage uptime, sourcing mitigation is increasingly favored over inventory mitigation as disruptions become less frequent but longer. Further, we show that a mixed mitigation strategy (partial sourcing from the reliable supplier and carrying inventory) can be optimal if the unreliable supplier has finite capacity or if the firm is risk averse. Contingent rerouting is a possible tactic if the reliable supplier can ramp up its processing capacity, that is, if it has volume flexibility. We find that contingent rerouting is often a component of the optimal disruption-management strategy, and that it can significantly reduce the firm’s costs. For a given percentage uptime, mitigation rather than contingent rerouting tends to be optimal if disruptions are rare.
Human endothelial cells generate Th17 and regulatory T cells under inflammatory conditions (2011)

Organ transplantation represents a unique therapeutic option for irreparable organ dysfunction and rejection of transplants results from a breakdown in operational tolerance. Although endothelial cells (ECs) are the first target in graft rejection following kidney transplantation, their capacity to alloactivate and generate particular T lymphocyte subsets that could intervene in this process remains unknown. By using an experimental model of microvascular endothelium, we demonstrate that, under inflammatory conditions, human ECs induced proliferation of memory CD4(+)CD45RA(-) T cells and selectively amplified proinflammatory Th17 and suppressive CD45RA(-)HLA-DR(+)FoxP3(bright) regulatory CD4(+) T lymphocytes (Tregs). Although HLA-DR expression on resting microvascular ECs was sufficient to induce proliferation of memory CD4(+) T cells, Treg amplification was dependent on the interaction with CD54, highly expressed only under inflammatory conditions. Moreover, expansion of Th17 cells was dependent on IL-6 and STAT-3, and inhibition of either specifically impaired Th17, without altering Treg expansion. Collectively these data reveal that the HLA-DR(+) ECs regulate the local inflammatory allogeneic response, promoting either an IL-6/STAT-3-dependent Th17 response or a contact-CD54-dependent regulatory response according to the cytokine environment. Finally, these data open therapeutic perspectives in human organ transplantation based on targeting the IL-6/STAT-3 pathway and/or promoting CD54 dependent Treg proliferation.
Molecular Cloning and Characterization of Senescence-Related Genes from Carnation Flower Petals (1989)

The senescence of carnation (Dianthus caryophyllus L.) flower petals is associated with increased production of ethylene which plays an important role in regulating this developmental event. Three senescence-related cDNA clones were isolated from a cDNA library prepared from mRNA isolated from senescing petals. These cDNAs are representative of two classes of mRNAs which increase in abundance in senescing petal tissue. The mRNA for one class is present at low levels during the early stages of development and begins to accumulate in mature petals prior to the increase in ethylene production. The accumulation of this mRNA is reduced, but not eliminated, in petals treated with aminooxyacetic acid, an inhibitor of ethylene biosynthesis, or silver thiosulfate, an ethylene action inhibitor. In contrast, expression of the second class of mRNAs appears to be highly regulated by ethylene. These mRNAs are not detectable prior to the rise in ethylene production and increase in abundance in parallel with the ethylene climacteric. Furthermore, expression of these mRNAs is significantly inhibited by both aminooxyacetic acid and silver thiosulfate. Expression of these mRNAs in vegetative and floral organs was limited to floral tissue, and predominantly to senescing petals.
Type I Interferons Inhibition of Inflammatory T Helper Cell Responses in Systemic Lupus Erythematosus (2007)

T helper (Th) cells play a central role in systemic lupus erythematosus (SLE). Activated autoreactive Th cells provide the help required for autoreactive B cells to differentiate and produce pathogenic autoAbs. Both autoAb-containing immune complexes and direct effects of inflammatory Th cells promote tissue injury and organ damage. In SLE, triggering of plasmacytoid dendritic cell (pDC) Toll-like receptors by autoimmune complexes containing nucleic acid autoantigens stimulates pDC secretion of high levels of type I interferons (IFN-alpha/beta). Study of SLE patients and murine disease models implicate these type I IFNs as key disease effectors. However, the role of pDC-derived type I IFNs in regulating the inflammatory function of Th cells in SLE is unknown. Although, type I IFNs are classically considered to promote Th1-mediated inflammation, they can also act as potent inhibitors of both Th1 and Th17 inflammatory cell responses. Work of ourselves and others leads us to hypothesize that if initiated during stages of SLE when Th cell-mediated tissue inflammation is absent or minimal, such as early in the disease or during periods of remission, type I IFN neutralization will disrupt the cycle of systemic autoimmune induction and disease. However, if initiated during advanced stages of disease when there is substantial ongoing Th1 (and possibly Th17) cell-mediated inflammation, targeting type I IFNs will exacerbate the Th cell-mediated inflammatory disease and thus potentiate end-organ damage and destruction. This has important implications for the application of the numerous anti-type I IFN therapies currently under development for SLE treatment.
Dielectric polymers embedded with high-k coated conducting particles: effective dielectric properties modeling (2005)

High dielectric constant (high-k) materials are needed for embedded passives in advanced packaging applications. This work presents a systematic theoretical investigation of the effective dielectric constant, dielectric loss of dielectric polymers filled with high-k coated conducting particles with the objective to aid the design of polymer/filler composite materials with high dielectric constant and low dielectric loss at high frequencies. Our results demonstrate that, contrary to prevailing views, the filler concentration and coating layer thickness dependences of the effective dielectric constant and loss are nonmonotonic. Implications for high-k materials design are also outlined.
Antiphlogistische 2,3‐Dihydro‐1H‐pyrrolizine, 14. Mitt. Stellungsisomere Diaryldihydropyrrolizinyl‐ameisensäuren und ‐propionsäuren (1988)

Abstract 5,6‐, 6,7‐ und 5,7‐diarylierte 2,3‐Dihydropyrrolizinyl‐ameisensäuren sowie die entspr. Propionsäurederivate werden nach verschiedenen Methoden synthetisiert und anhand ihrer spektroskopischen Daten charakterisiert.