gebhart/patrepeval huggingface dataset:
Identification and Strain Differentiation of <i>Vibrio cholerae</i> by Using Polyclonal Antibodies against Outer Membrane Proteins (2001)

Cholera is caused only by O1 and O139 Vibrio cholerae strains. For diagnosis, 3 working days are needed for bacterial isolation from human feces and for biochemical characterization. Here we describe the purification of bacterial outer membrane proteins (OMP) from V. cholerae O1 Ogawa, O1 Inaba, and O139 strains, as well as the production of specific antisera and their use for fecal Vibrio antigen detection. Anti-OMP antisera showed very high reactivity and specificity by enzyme-linked immunosorbent assay (ELISA) and dot-ELISA. An inmunodiagnostic assay for V. cholerae detection was developed; this assay avoids preenrichment and costly equipment and can be used for epidemiological surveillance and clinical diagnosis of cases, considering that prompt and specific identification of bacteria is mandatory in cholera.
Sialoadhesin (CD169) mRNA expression in peripheral blood mononuclear cells is increased in patients with advanced cancer (2006)

3643 Epstein-Barr virus (EBV) encoded small RNA (EBER) and antigens were strongly and specifically expressed in primary tumor tissues from nasopharyngeal cancer (NPC) patients. Its viral DNA was also differentially expressed in the serum or plasma samples from NPC patients. In recent years, the use of the circulating serum/plasma EBV DNA in diagnosis, predicting of relapse and prognostication of NPC has been strongly advocated by many research institutes. This study examined the protein expression of 4 additional genes in consecutive tumor biopsy tissues from NPC patients by immunohistochemical staining and their circulating serum DNA levels by real time PCR intending to find more markers for diagnosis of NPC. Abundant protein expressions of the 4 genes, namely, Bcl-2 (apoptosis inhibitory gene), p53 (tumor suppressor gene) and CXCR4 and CCR7 (chemokine receptors) were found in NPC biopsy tissues with Bcl-2 protein specifically present in the tumor cells in a similar fashion as EBER and p53 protein residing predominantly in the tumor cells as well as some apparently normal epithelium. The 2 chemokine receptors were expressed in the tumor cells as well as the stromal and epithelial cells. We further investigated the circulating nucleic acid levels of these genes in parallel with EBV DNA in the serum samples from 50 NPC patients and 50 normal controls. The 4 circulating DNAs were all dramatically and differentially elevated in NPC patients versus the normal controls in a very similar manner as EBV DNA. However, unlike EBV DNA, these circulating DNAs were also elevated in serum samples from lung cancer as well as diffuse large B cell lymphoma. We anticipated that these newly discovered circulating DNA could be useful for future diagnosis of NPC and other cancers.
Is Hydroxyapatite Ceramic Included in the Bone Remodelling Proccess? An In Vitro Study of Resorption and Formation Processes (2007)

Bone remodelling is a coupled process of bone formation and resorption. This process is physiologically controlled and involves the synthesis of bone matrix by osteoblasts and bone resorption by giant cells called osteoclasts. It includes a complex interaction of cells, with specific chemokine signalling. In order to study bone remodelling in vitro, we cultivated two precursor cellline types in a model coculture system on synthetic hydroxyapatite ceramic (HA) for 14 days. The monocytes have the capacity to differentiate into osteoclast-like cells, and the bone marrow stromal cells can differentiate into osteoblast-like cells. This coculture was used to analyse the in vitro cell interaction between monocytes and stromal cells. Furthermore, the attachment of the bone marrow stromal cells to the resorbed HA-surfaces was studied. In this in vitro study we demonstrated osteoclast-like differentiation and bone marrow stromal growth in a coculture system on a synthetic bone substitute material. Under optimal conditions, HA can be resorbed and bone marrow cells can grow into the lacunas to form new bone. These results give important cues for the adjustment of synthetic bone substitute materials for optimal remodelling behaviour.
A direct method for determining the structure of chromium(III) complexes in solution by deuteron nuclear magnetic resonance (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA direct method for determining the structure of chromium(III) complexes in solution by deuteron nuclear magnetic resonanceWilliam D. Wheeler, Sumio Kaizaki, and J. Ivan LeggCite this: Inorg. Chem. 1982, 21, 8, 3248–3250Publication Date (Print):August 1, 1982Publication History Published online1 May 2002Published inissue 1 August 1982https://pubs.acs.org/doi/10.1021/ic00138a069https://doi.org/10.1021/ic00138a069research-articleACS PublicationsRequest reuse permissionsArticle Views81Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Frequency-shaped sliding mode control using H/sub ∞/ control and μ synthesis theory (2002)

This paper proposes a new sliding mode control method using H/sub /spl infin////spl mu/ control theory and shows its applications. This concept is based on the frequency-shaped approach. A conventional hyperplane consists of a desired reference model without dynamics. Therefore, the sliding mode control system often becomes unstable based on spillover phenomena in a higher frequency region. On the other hand, the proposed design method can completely suppress such spillover phenomena because of the frequency-shaped hyperplane. Also, it has good robustness and robust performance in cases of parameter variations on the hyperplane to minimize the maximum singular value and structured singular value from some noise to the controlled variables. We have applied this new method to the flexible structure of the miniature test rig with four stories like high rise building and the positioning control system with the flexible arm. We have verified from simulations and experiments that the new sliding mode control method proposed in previous paper has good performances and it is very useful to suppress the spillover in a higher frequency region.
Development of an equation of state fully implicit compositional model. (2000)

A fully implicit compositional model is developed following the Coats approach. Although the fully implicit formulation guarantees a high degree of computational stability, it aggravates the computational efficiency due to the large number of independent variables. To relax this deficiency, a phase-stability analysis routine is included in the model. Adopting the Gibbs free energy as a stability criterion, we can estimate the number of phase present, prior to the flash calculation. If a single phase is detected, the subsequent flash computation routine can be detoured.The developed model is verified against an established model, an adaptive implicit compositional simulator, GEM. The computationa efficiencies with and without the stability analysis are evaluated to confirm the improvement in the iterative flash calculations. When there exists only one phase, the number of iterations is largely reduced by virtue of the stability analysis. In addition, even in two-phase situations. the flash computation is efficiently performed, because the stability analysis yields proper guesses. It is revealed that the stability analysis improves the computational efficiency regardless of the number of phases.
Simultaneous determination of inorganic anions and cations by ion chromatography with EDTA (ethylenediaminetetraacetic acid) as eluent (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSimultaneous determination of inorganic anions and cations by ion chromatography with EDTA (ethylenediaminetetraacetic acid) as eluentManabu. Yamamoto, Hirofumi. Yamamoto, Yuroku. Yamamoto, Susumu. Matsushita, Nobuyuki. Baba, and Tetsuo. IkushigeCite this: Anal. Chem. 1984, 56, 4, 832–834Publication Date (Print):April 1, 1984Publication History Published online1 May 2002Published inissue 1 April 1984https://pubs.acs.org/doi/10.1021/ac00268a061https://doi.org/10.1021/ac00268a061research-articleACS PublicationsRequest reuse permissionsArticle Views327Altmetric-Citations54LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Sorting by the Cytoplasmic Domain of the Amyloid Precursor Protein Binding Receptor SorLA (2007)

SorLA/LR11 (250 kDa) is the largest and most composite member of the Vps10p-domain receptors, a family of type 1 proteins preferentially expressed in neuronal tissue. SorLA binds several ligands, including neurotensin, platelet-derived growth factor-bb, and lipoprotein lipase, and via complex-formation with the amyloid precursor protein it downregulates generation of Alzheimer's disease-associated Abeta-peptide. The receptor is mainly located in vesicles, suggesting a function in protein sorting and transport. Here we examined SorLA's trafficking using full-length and chimeric receptors and find that its cytoplasmic tail mediates efficient Golgi body-endosome transport, as well as AP-2 complex-dependent endocytosis. Functional sorting sites were mapped to an acidic cluster-dileucine-like motif and to a GGA binding site in the C terminus. Experiments in permanently or transiently AP-1 mu1-chain-deficient cells established that the AP-1 adaptor complex is essential to SorLA's transport between Golgi membranes and endosomes. Our results further implicate the GGA proteins in SorLA trafficking and provide evidence that SNX1 and Vps35, as parts of the retromer complex or possibly in a separate context, are engaged in retraction of the receptor from endosomes.
Radiation therapy for acquired immunodeficiency syndrome-related Kaposi's sarcoma. (1988)

Twenty-four patients with acquired immunodeficiency syndrome (AIDS) received 80 courses of radiation therapy for Kaposi's sarcoma (KS). Pain and other symptoms due to mass effects were well controlled, but KS lesions often persisted in irradiated sites. Acute radiation toxicity to doses of approximately 2,000 cGy in ten fractions to the oral cavity and/or the foot were significant and may limit the dose and the efficacy of radiotherapy in patients with the epidemic form of KS.
Towards dynamically configurable context recognition systems (2012)

General representation, abstraction and exchange def-initions are crucial for dynamically configurable con-text recognition. However, to evaluate potential defini-tions, suitable standard datasets are needed. This paper presents our effort to create and maintain large scale, multimodal standard datasets for context recognition re-search. We ourselves used these datasets in previous research to deal with placement effects and presented low-level sensor abstractions in motion based on-body sensing. Researchers, conducting novel data collections, can rely on the toolchain and the the low-level sensor abstrac-tions summarized in this paper. Additionally, they can draw from our experiences developing and conducting context recognition experiments. Our toolchain is already a valuable rapid prototyping tool. Still, we plan to extend it to crowd-based sensing, enabling the general public to gather context data, learn more about their lives and contribute to context recog-nition research. Applying higher level context reasoning on the gathered context data is a obvious extension to our work.
Direct Partial Oxidation of Natural Gas to Liquid Chemicals: Chemical Kinetic Modeling and Global Optimization (2008)

The direct homogeneous partial oxidation of methane to methanol is investigated through modeling and a limited set of experiments. The experiments are conducted in a laminar flow quartz reactor at 100 bar and 598−763 K under undiluted conditions. The modeling is performed with a detailed chemical kinetic model, previously validated against a range of high-pressure data on oxidation of CO/H2 and small hydrocarbons. Modeling predictions compare well also with the experimental data obtained in the present study. On the basis of the chemical kinetic modeling, the effect of the main process parameters is discussed and the process is optimized, using a numerical global optimization methodology based on interval analysis. The experimental data, as well as the optimization results, indicate yields of methanol in the upper range of those reported in literature. However, the values are well below the commercial target.
Metallo-organic chemistry (1985)

Bonding in Transition Metal Organometallic Complexes Some Common Properties and Reactions of Transition Metal Organometallic Complexes s-Alkyl Complexes Carbene and Carbyne Complexes n2-Alkene and Alkyne Complexes n3-Allyl Complexes n4-Diene Complexes n5-Dienyl Complexes n6-Arene and n6-Triene Complexes Index.
A Survey of Multiple Tree Visualisation (2009)

This article summarises the current state of research into multiple tree visualisations. It discusses the spectrum of current representation techniques used on single trees, pairs of trees and finally multiple trees, in order to identify which representations are best suited to particular tasks and to find gaps in the representation space, in which opportunities for future multiple tree visualisation research may exist. The application areas from where multiple tree data are derived are enumerated, and the distinct structures that multiple trees make in combination with each other and the effect on subsequent approaches to their visualisation are discussed, along with the basic high-level goals of existing multiple tree visualisations.
Coordination of Cooperative Autonomous Vehicles: Toward safer and more efficient road transportation (2016)

While intelligent transportation systems come in many shapes and sizes, arguably the most transformational realization will be the autonomous vehicle. As such vehicles become commercially available in the coming years, first on dedicated roads and under specific conditions, and later on all public roads at all times, a phase transition will occur. Once a sufficient number of autonomous vehicles is deployed, the opportunity for explicit coordination appears. This article treats this challenging network control problem, which lies at the intersection of control theory, signal processing, and wireless communication. We provide an overview of the state of the art, while at the same time highlighting key research directions for the coming decades.
Incremental Linear Discriminant Analysis for Classification of Data Streams (2005)

This paper presents a constructive method for deriving an updated discriminant eigenspace for classification when bursts of data that contains new classes is being added to an initial discriminant eigenspace in the form of random chunks. Basically, we propose an incremental linear discriminant analysis (ILDA) in its two forms: a sequential ILDA and a Chunk ILDA. In experiments, we have tested ILDA using datasets with a small number of classes and small-dimensional features, as well as datasets with a large number of classes and large-dimensional features. We have compared the proposed ILDA against the traditional batch LDA in terms of discriminability, execution time and memory usage with the increasing volume of data addition. The results show that the proposed ILDA can effectively evolve a discriminant eigenspace over a fast and large data stream, and extract features with superior discriminability in classification, when compared with other methods.
Wind farm modelling for power quality (2002)

In this paper, the power quality impact of wind farms on the power system is investigated. A model is established for the wind farm Hagesholm (Denmark). This wind farm consists of six active stall controlled wind turbines from NEG-Micon with 2 MW rated power. The wind turbines are connected through two feeders to the 10kV busbar in a 50/10kV substation. The simulation model, implemented in the dedicated power system simulation tool DIgSILENT, is used in the first place to simulate the behaviour of the wind farm during normal operation of the wind farm. The model comprises a grid model, six almost identical wind turbine models, where electric, mechanic and aerodynamic aspects are included, and a model for the wind speed. The described model can be used for assessment of power quality, control strategies and behaviour in the event of grid faults. In this paper, the ability of the model to predict power quality is investigated. The simulations are used to compute power quality characteristics of a wind turbine, i.e. maximum power, reactive power consumption and flicker emission. The applied computation methods are reflecting the international power quality standards of wind turbine IEC 61400-21. The computed power quality characteristics are compared to measured power quality characteristics, determined by the same computation methods.
A one dose experimental cholera vaccine (2012)

The number of cholera vaccine doses required for immunity is a constraint during epidemic cholera. Protective immunity following one dose of multiple Vibrio cholerae (Vc) colonization factors (Inaba LPS El Tor, TcpA, TcpF, and CBP-A) has not been directly tested even though individual Vc colonization factors are the protective antigens. Inaba LPS consistently induced vibriocidal and protective antibodies at low doses. A LPS booster, regardless of dose, induced highly protective secondary sera. Vc protein immunogens emulsified in adjuvant were variably immunogenic. CBP-A was proficient at inducing high IgG serum titers compared with TcpA or TcpF. After one immunization, TcpA or TcpF antisera protected only when the toxin co-regulated pilus operon of the challenge Vc was induced by AKI culture conditions. CBP-A was not consistently able to induce protection independent of the challenge Vc culture conditions. These results reveal the need to understand how best to leverage the 'right' Vc immunogens to obtain durable immunity after one dose of a cholera subunit vaccine. The dominance of the protective anti-LPS antibody response over other Vc antigen antibody response needs to be controlled to find other protective antigens that can add to anti-LPS antibody-based immunity.
A Practical Indium Tribromide Catalysed Addition of Indoles to Nitroalkenes in Aqueous Media (2002)

The 1,4-conjugate addition of indoles to nitroalkenes was efficiently carried out in aqueous media using a catalytic amount of indium tribromide (5 mol%). The reusability of the indium tribromide was tested by performing consecutive cycles with the same catalyst.
Expression and Functional Study of Wild-Type and Mutant Human Cytochrome P450c21 in <i>Saccharomyces cerevisiae</i> (1991)

The most common cause of congenital adrenal hyperplasia is deficiency of cytochrome P450c21 (21-hydroxylase), which catalyzes the synthesis of adrenal steroids. We have cloned the human P450c21 cDNA into yeast expression vectors under the control of either the glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) promoter or the aldehyde-dehydrogenase (ADH) promoter. P450c21 RNA, protein, and enzyme activity can be detected, indicating that both promoters drive the synthesis of P450c21. The expressed P450c21 catalyzes the conversion of both of its substrates, with Km and Vmax values of 0.33 microM and 280 nmoles/hr.nmole of P450c21 protein for progesterone, and 0.23 microM and 450 nmoles/hr.nmole for 17-hydroxyprogesterone. These kinetic properties are similar to those of human P450c21 expressed in COS-1 cells. The microsomal fraction containing P450c21 exhibited an absorption peak at 450 nm upon binding to CO, demonstrating its hemoprotein nature. The CO-difference spectra indicated that there were about 0.08 nmole P450c21 hemoprotein/mg microsomal protein. Coupling this expression system with site-directed mutagenesis, the Asn-172 mutant of P450c21 had about 20-100 lower Vmax values; yet it retained normal affinity toward both substrates. This mutant protein also exhibited an altered absorbance with a peak at 420 nm rather than at 450 nm.
Tensegrity Systems and Geodesic Domes (1990)

The name Buckminster Fuller is associated with the development of geodesic domes and with tensegrity systems. The principle of the latter is that rigidity is obtained by selfstressing alone. The shapes of domes constructed using tensegrity systems are not generally known a priori; these domes belong to the class of systems with indeterminate shape, such as cable systems. A form-finding method using the dynamic relaxation technique with attenuation of kinetic energy is presented here. The results given by this general method are compared with known solutions in certain cases and make it possible to quantify the deviations between geometries of tensegrity systems and polyhedral geometries. An example is given to illustrate the possibilities of the combination of the principle of tensegity systems with that of geodesic domes. It is a double-layer, double-curvature system whose shape respects the selfstressing criterion. Industrial construction of the system can be envisaged thanks to the design of an assembly node which permits variations in curve radius.
Synthesis and Exploratory Deposition Studies of Isotetrasilane and Reactive Intermediates for Epitaxial Silicon (2019)

A synthetic method is presented for the production of isotetrasilane, a higher order perhydridosilane, with the purity and volume necessary for use in extensive studies of the chemical vapor deposition (CVD) of epitaxial silicon (e-Si) thin films. The chemical characteristics, thermodynamic properties, and epitaxial film growth of isotetrasilane are compared with those of other perhydridosilanes. A film-growth mechanism distinct from linear perhydridosilanes H(SiH<sub>2</sub>) <sub>n</sub>H, where n ≤ 4, is reported. Preliminary findings are summarized for CVD of both unstrained e-Si and strained e-Si doped with germanium (Ge) and carbon (C) employing isotetrasilane as the source precursor at temperatures of 500-550 °C. The results suggest that bis(trihydridosilyl)silylene is the likely deposition intermediate under processing conditions in which gas-phase depletion reactions are not observed.