gebhart/patrepeval huggingface dataset:
Phosphorylation of p27Kip1 at Threonine 198 by p90 Ribosomal Protein S6 Kinases Promotes Its Binding to 14-3-3 and Cytoplasmic Localization (2003)

The cyclin-dependent kinase inhibitor p27Kip1 plays an important role in cell cycle regulation. The cyclin-dependent kinase-inhibitory activity of p27Kip1 is regulated by changes in its concentration and its subcellular localization. Several reports suggest that phosphorylation of p27Kip1 at serine 10, threonine 157, and threonine 187 regulate its localization. We have previously identified that carboxyl-terminal threonine 198 (Thr198) in p27Kip1 is a novel phosphorylation site and that Akt is associated with the phosphorylation at the site (Fujita, N., Sato, S., Katayama, K., and Tsuruo, T. (2002) J. Biol. Chem. 277, 28706-28713). We show herein that activation of the Ras/Raf/mitogen-activated protein kinase kinase (MAPK kinase/MEK) pathway also regulates phosphorylation of p27Kip1 at Thr198. MAPKs were not directly associated with p27Kip1 phosphorylation at Thr198, but the p90 ribosomal protein S6 kinases (RSKs) could bind to and directly phosphorylate p27Kip1 at Thr198 in a Ras/Raf/MEK-dependent manner. RSK-dependent phosphorylation promoted the p27Kip1 binding to 14-3-3 and its cytoplasmic localization. To prove the direct relationship between 14-3-3 binding and cytoplasmic localization, we constructed a p27Kip1-R18 fusion protein in which the R18 peptide was fused to the carboxyl-terminal region of p27Kip1. The R18 peptide is known to interact with 14-3-3 independent of phosphorylation. The p27Kip1-R18 distributed mainly in the cytosol, whereas mutant p27Kip1-R18 (p27Kip1-R18-K2) that had no 14-3-3 binding capability existed mainly in the nucleus. These results indicate that RSKs play a crucial role in cell cycle progression through translocation of p27Kip1, in addition to Akt, to the cytoplasm in a phosphorylation and 14-3-3 binding-dependent manner.
Meeting Real-Time Constraints in CAN (2005)

This paper deals with the problem of information flow scheduling in a communication system based on CAN ISO IS-11898 physical medium access mechanism. It mainly features a bus access arbitration protocol based on a priority assigned to each message to be transmitted; if two or more messages are transmitted at the same time by different communication nodes, only the message with the highest priority continues to be transmitted, the other being stopped. In real-time applications, messages are relevant to process variables which must be transmitted within strict time constraints; according to the CAN ISO IS-11898 bus arbitration protocol, respect of real-time constraints depends on the priority assigned to each variable. The aim of the paper is to propose a procedure for dynamic assignment of priorities to variables to be transmitted, in such a way the relevant real-time requirements are fulfilled. Although many other approaches can be found in literature, the proposal is original as it is based on standard full CAN communication stacks.
Growth and properties of dilute magnetic semiconductor superlattices containing Hg1−<i>x</i>Mn<i>x</i>Te (1986)

We report details of the successful growth by molecular beam epitaxy of superlattices containing alternating layers of Hg1−xMnxTe and HgTe. These structures are the first superlattices containing layers of a mercury-based dilute magnetic semiconductor (Hg1−xMnxTe ) to be grown by any thin-film technique. The optical and electrical properties of these new magnetic multilayers are discussed.
Local Anesthetics Containing the ac-Tetrahydro-beta-naphthylamine Pressor Group<sup>1</sup> (1936)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLocal Anesthetics Containing the ac-Tetrahydro-beta-naphthylamine Pressor Group1Harold W. Coles and William A. LottCite this: J. Am. Chem. Soc. 1936, 58, 10, 1989–1990Publication Date (Print):October 1, 1936Publication History Published online1 May 2002Published inissue 1 October 1936https://pubs.acs.org/doi/10.1021/ja01301a049https://doi.org/10.1021/ja01301a049research-articleACS PublicationsRequest reuse permissionsArticle Views28Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
High level production and rapid purification of the<i>E. coli trp</i>repressor (1986)

Two small, multicopy, expression plasmids were constructed that permit convenient insertion of trpR, the structural gene for the trp repressor of Escherichia coli, with its natural ribosome binding site or adjacent to the ribosome binding site for the trp leader peptide. In these plasmids trpR is positioned between the strong regulated tac promoter and the rpoC transcription terminator. IPTG induction of lacIq strains bearing these plasmids results in the production of 25-50% of the soluble cell protein as trp repressor. Mutant and wild type repressors overproduced in this manner have been purified by simple procedures.
A Novel Micro-Tissue Reactor for Maintaining Hepatocytes in Vitro (2006)

We apply the biomimetic engineering concept to design a microfluidic device which mimics the flow field of the real functional liver tissue. HepG2/C3A in microreactor not only possessed the ultrastructure of tight junctions and apical microvilli, but also arranged as hepatic cord-like morphology under designed fluids field. In addition, albumin synthesis and urea production were also enhanced in microreactors compared to those in conventional Petri dish cultures. The microfluid cell culture array could potentially be applied in a wide range such as high throughput cell-based screening, drug screening, personal cell therapy and quantitative cell biology.
Ni(II)-Bearing Green Rust II and Its Spontaneous Transformation into Ni(II)-Bearing Ferrites (1986)

Abstract When solutions containing Fe(II), Ni(II), and SO42− ions were oxidized by a mixed gas of air and nitrogen at pH 7.1 (25 °C), a Ni(II)-bearing green rust II (GR-II), which is a uniform crystalline precipitate with a definite chemical composition, was formed. The Ni(II) content in the Ni(II)-bearing GR-II increased with an increase in the Ni(II) concentration in the reaction solution. The Ni(II)-bearing GR-II was spontaneously transformed into Ni(II)-bearing ferrite under a nitrogen atmosphere without any oxidant. The metal mole ratio of [Ni(II)/ Fe(III)]fer in the ferrite increased linearly with an increase in that of [Ni(II)/Fe(III)]grn in the Ni(II)-bearing GR-II (slope=0.193). From this relationship, the spontaneous transformation reaction was described as 2(Ni2+)x(Fe2+)2−x(Fe3+)(SO42−)(OH−)5→Ni0.39xFe3−0.39xO4+(2−1.61x)Fe2++1.61xNi2++Fe(OH)2+2SO42−+4H2O. This equation shows that Fe(II) ion in the GR-II is more readily incorporated into the ferrites than Ni(II) ion.
Preconditioning by Mitochondrial ATP-Sensitive Potassium Channel Openers : An Effective Approach for Improving the Preservation of Heart Transplants (1999)

These data support the concept that the cardioprotective effects of ischemic preconditioning can be duplicated by a mitochondrial K(ATP) opener and suggest that activation of these channels could be an effective means of improving the preservation of globally ischemic cold-stored hearts, as occurs during cardiac transplantation.
Molecular Beam Epitaxial Growth of Al-doped ZnMgO Alloy Films for Modulation-doped ZnO/ZnMgO Heterostructures (2005)

In this paper, we describe the characteristics of Al-doped n -type Zn 1- x Mg x O alloy films grown on a -plane sapphire substrates by molecular beam epitaxy, and the application of the films in modulation-doped ZnO/Zn 1- x Mg x O quantum wells (QWs). The results of Hall measurement for the Al-doped Zn 0.8 Mg 0.2 O alloy films revealed an excellent doping efficiency that the resistivity at 300 K decreased from 3.8×10 -1 Ω·cm at 1.0×10 18 cm -3 to 8.0×10 -4 Ω·cm at 3.5×10 20 cm -3 . Although Al doping at higher than 10 20 cm -3 resulted in a reduction in intensity and a broadening of the peak width of near-band-edge emission in cathodoluminescence with an increase in absorption-edge energy induced by the Burstein–Moss shift in optical transmittance, highly c -axis-oriented films without rotational domains were obtained in a wide range of doping levels. Such a successful doping was also confirmed for Zn 1- x Mg x O alloy films with a Mg content as high as 0.4. By applying Al doping to modulation-doped ZnO/Zn 0.6 Mg 0.4 O QWs, the sheet carrier density of the ZnO well was found to be proportional to the doping level in the Zn 0.6 Mg 0.4 O barrier layer.
[Effects of rice-duck farming system on Oryza sativa growth and its yield]. (2005)

The study showed that under rice-duck farming, the number of rice non-productive tiller reduced significantly, the ratio of its effective panicles increased by 8.08 %, and its basal penetration light rate enhanced by 4.05%. At full-heading and maturing stages, the green leaf ratio under rice-duck farming was 6.01% and 10.65% higher than the control, and the leaf chlorophyll content was increased by 2.90% and 17.82%, respectively. Under rice-duck farming, the root vigour at full-heading stage and the photosynthesis capability of flag leaf at grain-filling stage were increased by 24.2% and 15.73%, which could accumulate more assimilative matter, and increase the harvest index and yield by 2.87% and 4.93%, respectively.
Benign and malignant stenoses of the stomach and duodenum: treatment with self-expanding metallic endoprostheses. (1996)

The placement of Wallstents offers good palliation of inoperable outlet stenoses of the stomach and the duodenum. With a combined radiologic and endoscopic approach, it is an easy and rapid procedure that can be performed without general anesthesia.
A yeast assay probes the interaction between botulinum neurotoxin serotype B and its SNARE substrate (2006)

The seven functionally distinct serotypes (A-G) of botulinum neurotoxin (BoNT) are dichains consisting of light chain (LC) with zinc-dependent endoprotease activity connected by one disulfide bond to heavy chain with neuronal-cell translocation and receptor-binding domains. LC-mediated proteolysis of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins and consequent inhibition of synaptic vesicle fusion to the presynaptic membrane of human motor neurons are responsible for flaccid paralysis associated with botulism. LC endoproteolysis is complex, requiring highly extended SNARE sequences at the surface of intracellular membranes and prompting our development of a genetically amenable assay to monitor the interaction between BoNT/LC and its SNARE substrate. Using BoNT serotype B as a model, the assay employs a chimeric SNARE protein where a portion of neuronal synaptobrevin (Sb) is fused to Snc2p, a Sb ortholog required for protein secretion from yeast cells. Regulated expression of serotype B-LC in yeast leads to cleavage of the chimera and a conditional growth defect. To assess utility of this assay for monitoring SNARE protein cleavage, we growth-selected chimeric SNARE mutations that inhibited proteolysis. When these mutations were introduced into Sb and examined for cleavage, substrate residues located near and distal to the cleavage site were important, including residues positioned near the Sb transmembrane domain, an unexplored aspect of BoNT cell intoxication. Additional mutations were positioned in a nine-residue SNARE motif, supporting a previously assigned role for this motif in LC recognition and providing proof of principle for the application of yeast-based technology to study intracellular BoNT/LC endoproteases.
All-trans-retinoic Acid Increases Transforming Growth Factor-β2 and Insulin-like Growth Factor Binding Protein-3 Expression through a Retinoic Acid Receptor-α-dependent Signaling Pathway (1997)

Retinoids, including retinol and retinoic acid derivatives, maintain the normal growth and differentiation of human bronchial epithelial cells. The signaling pathways through which retinoids mediate these effects have not been defined. Insulin-like growth factor binding protein-3 (IGFBP-3) and the transforming growth factor-beta (TGF-beta) gene family (beta1-3) were examined as potential components of the retinoid signaling pathway in normal human bronchial epithelial cells. All-trans-retinoic acid (t-RA) increased the levels of TGF-beta2 and IGFBP-3 mRNA and of secreted TGF-beta and IGFBP-3 proteins. An antagonist of retinoic acid receptor-alpha, LG100629, abrogated the increase in TGF-beta2 and IGFBP-3 mRNA levels induced by t-RA. t-RA increased IGFBP-3 mRNA levels transiently from 1 to 6 h, and subsequently a sustained increase began at 72 h, which coincided with the appearance of active TGF-beta in the media. Treatment with TGF-beta2 increased IGFBP-3 mRNA levels, but treatment with latency-associated peptide, which inactivates secreted TGF-beta, did not abrogate the effect of t-RA on IGFBP-3 expression. These findings provide evidence that t-RA increased TGF-beta2 and IGFBP-3 expression through an retinoic acid receptor-alpha-dependent pathway, and the increase in IGFBP-3 expression by t-RA did not require activation of the TGF-beta pathway by autocrine or paracrine mechanisms.
A Chromosomal Insertion Toolbox for Promoter Probing, Mutant Complementation, and Pathogenicity Studies in <i>Ralstonia solanacearum</i> (2011)

We describe here the construction of a delivery system for stable and directed insertion of gene constructs in a permissive chromosomal site of the bacterial wilt pathogen Ralstonia solanacearum. The system consists of a collection of suicide vectors-the Ralstonia chromosome (pRC) series-that carry an integration element flanked by transcription terminators and two sequences of homology to the chromosome of strain GMI1000, where the integration element is inserted through a double recombination event. Unique restriction enzyme sites and a GATEWAY cassette enable cloning of any promoter::gene combination in the integration element. Variants endowed with different selectable antibiotic resistance genes and promoter::gene combinations are described. We show that the system can be readily used in GMI1000 and adapted to other R. solanacearum strains using an accessory plasmid. We prove that the pRC system can be employed to complement a deletion mutation with a single copy of the native gene, and to measure transcription of selected promoters in monocopy both in vitro and in planta. Finally, the system has been used to purify and study secretion type III effectors. These novel genetic tools will be particularly useful for the construction of recombinant bacteria that maintain inserted genes or reporter fusions in competitive situations (i.e., during plant infection).
EVALUATION OF METHODOLOGY FOR NUTRITIONAL ASSESSMENT IN CHILDREN: Anthropometry, Body Composition, and Energy Expenditure (1997)

Nutritional status in children is an indicator of health and well-being at both the individual and the population level. Screening for malnutrition should be an integral part of pediatric care universally. Nutritional intervention requires repeated measurement of nutritional status to assess severity and to track progress over time. Methodological issues in the assessment of nutritional status are reviewed with emphasis on anthropometric measurement, body composition, and energy expenditure of children at risk for malnutrition. Use of reference data, measurement error, maturational effects, and hereditary factors are among the issues reviewed and serve as guidelines in the interpretation of measurement of nutritional status.
Imaging buddhist art with a digital large-format camera (2012)

This article describes recent field work undertaken by Microsoft Research Asia and the Dunhuang Academy to capture high-resolution images of Buddhist art at the UNESCO world heritage site, the Mogao Caves . This project is intended as a feasibility study examining the use of a digital large-format gigapixel camera to capture high-resolution images in a cultural heritage setting. In particular, we report on the current challenges faced by the Dunhuang Academy in their imaging efforts and how the use of a digital large-format camera can improve the quality of the imaging process while reducing time and effort. We also describe lessons learned from this field study as well as remaining challenges inherent to such projects.
Highly Compressible Cross-Linked Polyacrylamide Hydrogel-Enabled Compressible Zn–MnO<sub>2</sub> Battery and a Flexible Battery–Sensor System (2018)

The fast advancement in flexible and wearable electronics has put up with new requirements on the energy storage device with improved tolerance to deformation apart from offering power output. Despite the tremendous progress in stretchable energy storage devices, the compressional energy storage devices have indeed received limited research attention. In this work, an intrinsically compressible rechargeable battery was proposed using the Zn-MnO<sub>2</sub> chemistry and a cross-linked polyacrylamide hydrogel electrolyte. Interestingly, the battery exhibited not only good energy storage performances but also excellent tolerance against large compressional strain without sacrificing the energy storage capability. It was also found that the ionic conductivities of the hydrogel increased with the values of the compressional strain, leading to an enhanced electrochemical performance. More importantly, upon dynamic compression, the voltage output of the battery can be very stable and reliable. Consequently, the battery assembled using the hydrogel electrolyte can be used to power a luminescent panel even with a 3 kg load on top of it. It was also demonstrated that the flexible sensor powered by our compressible battery exhibited similar and stable sensory signals compared with the same sensor powered by two commercial alkaline batteries. Furthermore, because of the excellent mechanical property of our battery, a smart wristband fabricated by integrating two battery packs and the flexible piezoresistive sensor could be powered and used to monitor the pressure exerted, demonstrating the battery's potential as the wearable power source for the flexible and wearable devices.
Modeling and control of FAST-Hex: A fully-actuated by synchronized-tilting hexarotor (2016)

We present FAST-Hex, a novel UAV concept which is able to smoothly change its configuration from underactuated to fully actuated by using only one additional motor that tilts all propellers at the same time. FAST-Hex can adapt to the task at hand by finely tuning its configuration from the efficient (but underactuated) flight (typical of coplanar multi-rotor platforms) to the full-pose-tracking (but less efficient) flight, which is attainable by non-coplanar multi-rotors. We also introduce a novel full-pose geometric controller for generic multi-rotors (not only the FAST-Hex) that outperforms classical inverse dynamics approaches. The controller receives as input any reference pose in ℝ <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sup> ×SO(3) (3D position + 3D orientation). Exact tracking is achieved if the reference pose is feasible with respect to the propeller spinning rate saturations. In case of unfeasibility a new feasible desired trajectory is generated online giving priority to the positional part. The new controller is tested with the FAST-Hex but can be used for many other multi-rotor platforms: underactuated, slightly fully-actuated and completely fully-actuated.
Gaseous deoxidation of anode copper at the Noranda smelter (1972)

The evolution of methods used for deoxidation of anode topper at the Noranda smelter is summarized. The processes range from birch poles and reverberatory furnace to present day natural gas and rotary furnace. Current deoxidation practice is discussed in detail and comparisons made between Noranda and some alternative procedures. Résumé Les auteurs font une étude de l'évolution des methodes de désoxydation du cuivre à anode à la fonderie de Noranda. Ces méthodes vont du perchage et du four réverbére au four rotatif moderne au gaz naturel. Les méthodes courantes de désoxydation sont ana1ysées en détail et l'on compare les procédés de Noranda à certains autres.
Evaluation of the real-time Java runtime environment for deployment in time-critical systems (2009)

This paper evaluates the results of benchmarking an implementation of the Real-Time Specification for Java. First, a motivation is given, which describes the need for applications that realize different levels of timing behavior. The difficulties with such applications are described, also. Then tests for memory allocation, communication buffering, and timer accuracy, which are derived from those difficulties, are formulated. The test results are evaluated in the last section.
Rapid quantitative mineral and phase analysis using automated scanning electron microscopy (QemSCAN); potential applications in forensic geoscience (2004)

Abstract QemSCAN is a scanning electron microscope (SEM) system, initially designed to support the mining industry by providing rapid automated quantitative mineral analyses. The system is based upon Carl Zeiss SEMs fitted with up to four light-element energy dispersive X-ray spectrometers. Representative subsamples are mounted into either resin or wax blocks and polished prior to analysis, or can be mounted onto carbon tape. During analysis, X-ray spectra are collected at a user-defined pixel spacing and are acquired very rapidly ( c. 10 ms per pixel). The measured spectra are automatically compared against a database of known spectra and a mineral or phase name is assigned to each measurement point by the QemSCAN computer software programs. In this way the near-surface qualitative elemental composition of each particle is systematically mapped, assigned to a mineral name or chemical compound/species, and digital pixel maps of each particle are created. Depending upon a range of parameters, including the particle size and the user-defined pixel spacing (which can vary between 0.20 μm and 25 μm), approximately 1000 particles, each 1–10 μm in size, can be measured per hour using a 1 μm pixel spacing. In addition to providing a qualitative elemental analysis and mineralogical or phase assignment for each particle, data relating to particle size, shape and calculated specific density are also generated. In this study, the potential application of this automated SEM system in forensic geoscience was evaluated by the analysis of: (1) a series of soil samples, and (2) a series of dust samples from an industrial complex. In both case studies, the mine-ralogy/phase composition of each sample analysed was found to be distinctive. In addition, textural data for the soil samples and particle shape data for the dust samples show that they can be clearly distinguished. Automated SEM using QemSCAN has clear potential application in the analysis of soil or other trace evidence in forensic case work.
Some Observations of Recent Events (1926)

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