gebhart/patrepeval huggingface dataset:
Beta-adrenergic receptor-G protein-adenylate cyclase complex in experimental canine congestive heart failure produced by rapid ventricular pacing. (1991)

Changes in the beta-adrenergic receptor-G protein-adenylate cyclase complex were investigated in an experimental canine model of low-output heart failure produced by chronic rapid ventricular pacing. The contractile response occurring after exposure to the beta-adrenergic agonist dobutamine, measured as peak left ventricular + dP/dt, was decreased after 3 weeks of pacing. To further characterize the diminished functional responsiveness to beta-adrenergic receptor stimulation, beta-adrenergic receptor-adenylate cyclase coupling was investigated using membranes prepared from both control and paced animals. The density of beta-adrenergic receptors was decreased by 40% with a selective downregulation of the beta 1-subtype. The affinity of the receptor for the antagonist radioligand [125I]iodocyanopindolol remained unchanged. A defect in coupling was suggested by a decreased ability of isoproterenol, fluoride, and forskolin to stimulate adenylate cyclase in membranes prepared from failing hearts. Determination of the levels of Gi alpha (the alpha-subunit of Gi) by immunoblotting and pertussis toxin labeling revealed modest increases of approximately 30%. Furthermore, Mn2+ and purified Gs failed to stimulate adenylate cyclase in membranes prepared from failing hearts, indicating an impairment in the catalytic moiety of adenylate cyclase itself or in the ability of adenylate cyclase to couple to Gs. In contrast, complementation assay did not reveal differences in the functional activity of Gs alpha (the alpha-subunit of Gs). Taken together, these data demonstrate a selective decrease in the beta 1-subtype of adrenergic receptors and an increase in a 40-kd G1-like protein in the failing heart. Similar changes have been described in human idiopathic dilated cardiomyopathy. In addition to these changes, we identified a possible defect at the level of the catalytic subunit of adenylate cyclase.
Design of a Novel Open-Path Aerosol Extinction Cavity Ringdown Spectrometer (2015)

We describe a new open-path aerosol extinction cavity ringdown spectrometer (CRDS), which measures extinction coefficients as aerosol is drawn transversely through the optical cavity. With no inlet tubing, particle losses in the open-path CRDS due to impaction of coarse particles or evaporation of highly humidified particles in transfer lines are minimized, improving aerosol extinction measurements in dusty and humid environments. This report presents the key elements of the new open-path CRDS design as well as comparisons with a conventional closed-path CRDS and data obtained during a field study at NOAA's instrumented 300 m tower in Erie, Colorado. The open-path CRDS's 1σ detection limit for one second averaged data was 0.05 Mm−1, comparable to the limit of our closed-path CRDS.Copyright 2015 American Association for Aerosol Research
[Protein secretion of human cultured liver cells]. (1988)

Liver cells have many functions, and one of which is a production of plasma proteins. Therefore, studies on synthesis and production of plasma proteins from hepatocytes are very important for the recognition of various hepatic dysfunctions, clinically. Of late years, a lot of the complex mechanism of protein synthesis and--secretion was elucidated by using a technique of liver cell culture, for example, primary monolayer culture by freshly isolated hepatocytes and cloned cell culture derived from hepatocellular carcinoma. This paper described the results of our observations and other researchers, and then discussed the point of production of human major plasma proteins using the above culture methods, such as albumin, alpha-fetoprotein and transferrin. Furthermore, we showed statistically that half of twenty-six human hepatoma cell lines established until 1988 in Japan, had already lost their secretory potencies of major plasma proteins in vitro.
The E1 replication protein of bovine papillomavirus type 1 contains an extended nuclear localization signal that includes a p34cdc2 phosphorylation site (1993)

Bovine papillomavirus (BPV) DNA replication occurs in the nucleus of infected cells. Most enzymatic activities are carried out by host cell proteins, with the viral E1 and E2 proteins required for the assembly of an initiation complex at the replication origin. In latently infected cells, viral DNA replication occurs in synchrony with the host cell chromosomes, maintaining a constant average copy number of BPV genomes per infected cell. By analyzing a series of mutants of the amino-terminal region of the E1 protein, we have identified the signal for transport of this protein to the cell nucleus. The E1 nuclear transport motif is highly conserved in the animal and human papillomaviruses and is encoded in a similar region in the related E1 genes. The signal is extended relative to the simple nuclear localization signals and contains two short amino acid sequences which contribute to nuclear transport, located between amino acids 85 and 108 of the BPV-1 E1 protein. Mutations in either basic region reduce nuclear transport of E1 protein and interfere with viral DNA replication. Mutations in both sequences simultaneously prevent any observable accumulation of the protein and reduce replication in transient assays to barely detectable levels. Surprisingly, these mutations had no effect on the ability of viral genomes to morphologically transform cells, although the plasmid DNA in the transformed cells was maintained at a very low copy number. Between these two basic amino acid blocks in the nuclear transport signal, at threonine 102, is a putative site for phosphorylation by the cell cycle regulated kinase p34cdc2. Utilizing an E1 protein purified from either a baculovirus vector system or Escherichia coli, we have shown that the E1 protein is a substrate for this kinase. An E1 gene mutant at threonine 102 encodes for a protein which is no longer a substrate for the p34cdc2 kinase. Mutation of this threonine to isoleucine had no observable effect on either nuclear localization of E1 or DNA replication of the intact viral genome.
Modelling and searching web-based document collections (2002)

Jonker took place in my promotion committee.Besides Peter, I also found a willing ear in the persons of Jan Flokstra,
Citizen of London (1887)

Journal Article Citizen of London Get access A. Hall A. Hall Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume s7-III, Issue 62, 5 March 1887, Page 198, https://doi.org/10.1093/nq/s7-III.62.198f Published: 05 March 1887
Complex I and Parkinson's Disease (2001)

Complex I of the mammalian electron transfer chain is composed of at least 43 protein subunits, of which 7 are encoded by mtDNA. It catalyzes the transfer of electrons from NADH to ubiquinone and translocates protons from the mitochondrial matrix to the intermembrane space. It may also play direct roles in the mitochondrial permeability transition and in cell death pathways. Despite the limitations of current complex I assays, biochemical studies have suggested the presence of a mild, systemic defect of complex I in Parkinson's disease (PD). Recent experimental work has modeled this abnormality using rotenone to systemically inhibit complex I. Chronic rotenone exposure accurately recapitulated the pathological, biochemical, and behavioral features of PD. Thus, relatively subtle complex I abnormalities--either genetic or acquired--may be central to the pathogenesis of PD.
A theoretical appraisal of the relative merits of 50 % hybrid and synthetic varieties of grasses (1972)

SUMMARY The expected merits of the semi-hybrid variety, produced by bulk interpollination of two populations (Foster, 1971a, 6, c), are examined in relation to those of the synthetic variety. With diploid inheritance, the mean expression of both types of variety is seen to be the same. Any postulated difference in productivity must depend not only on the preferential establishment and survival of a small fraction of the total population, but on the existence of a correlation between characters determining productivity and establishment. For two populations producing a heterotic hybrid, a correlation due to loose linkage or gene association without linkage may result in the superiority of the semi-hybrid, but tight linkage or pleiotropy are more likely to favour the synthetic variety. It is concluded that superiority of the semi-hybrid mode of varietal production can only be established by means of a direct comparison with its synthetic counterpart, and not solely by the demonstration of upper-parent heterosis for productivity.
The Performance of Concurrency Control Algorithms for Database Management Systems (1984)

This paper describes a study of the performance of cen-tralized concurrency control algorithms. An algorithm-independent simulation framework was developed in order to support comparative studies of various concurrency control algorithms. We describe this framework in detail and present performance results which were obtained for what we believe to be a representative cross-section of the many proposed algo-rithms. The basic algorithms studied include four lock-ing algorithms. two timestamp algorithms. and one optimistic algorithm. Also. we briefly summarize stu-dies of several multiple version algorithms and several hierarchical algorithms. We show that. in general, locking algorithms provide the best performance.
Image Handling In Two-Dimensional Design (1982)

A research team in England has developed an image manipulation system that enables graphics designers to work creatively with visual ambiguities.
Effect of $\hbox{Ge}_{2}\hbox{Sb}_{2}\hbox{Te}_{5}$ Thermal Barrier on Reset Operations in Filament-Type Resistive Memory (2011)

We have investigated the effect of a <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\hbox{Ge}_{2}\hbox{Sb}_{2} \hbox{Te}_{5}$</tex></formula> (GST) thermal barrier on the reset operations in TiN/Cu/SiC/Pt devices. When the GST film was introduced as a thermal barrier, the device showed a lower reset voltage and a lower reset current than a device without the GST layer. In particular, the reset speed of the device with the GST layer was significantly faster at room temperature compared to the device without the GST layer. We attribute the improved resistive switching to the GST thermal barrier, which induces thermally assisted electrochemical reduction of the Cu filament.
Membrane pore architecture of a cytolytic toxin from Bacillus thuringiensis (2000)

To investigate the membrane pore structure of Cyt2Aa1 toxin from Bacillus thuringiensis, 14 single-cysteine substitutions of the toxin were constructed. Five of these mutants (L172C, V186C, L189C, E214C and L220C) yielded characteristic products when processed by proteinase K; other mutants were degraded by this enzyme. Mutants that yielded characteristic proteolysed products and wild-type toxin were labelled with polarity-sensitive acrylodan (6-acryloyl-2-dimethylaminonaphthalene) at the thiol group of cysteine residues. A green–blue shift in the emission spectra was observed with all labelled toxins on transfer from an aqueous solution into a solution containing membranes or liposomes from red blood cells. These results suggested that the label moved into the hydrophobic environment of the membrane or became buried within hydrophobic regions of the protein oligomers. Digestion of membrane-bound labelled toxin with proteinase K did not cause a significant decrease in emission intensity from any of the labelled mutants. This suggests that L172C, V186C, L189C, E214C and L220C are inserted into the membrane and are therefore protected from proteolysis. In contrast, a marked decrease in emission intensity was observed when membrane-bound labelled wild-type toxin was digested with proteinase K. This suggests that Cys-19 does not insert into the membrane. Fluorimetric analysis of delipidated pore complexes suggests that L172C, V186C, L189C and E214C point towards the lipid in the membrane, whereas L220C is either within the hydrophobic environment of the protein oligomers or exposed to the membrane lipids. Most of the Cys-19 from wild-type molecules is enclosed within the hydrophobic pockets of the protein oligomers.
Kinetic Behavior of Solid Particle in Chemical-Looping Combustion:  Suppressing Carbon Deposition in Reduction (1998)

In order to apply chemical-looping combustion to a practical power plant, carbon deposition on the solid particle is one of the key problems to be overcome. Six kinds of solid particles were examined to clarify the kinetic behavior of carbon deposition. The effects of the solid composition, feed gas composition, and reaction temperature on carbon deposition were investigated by TGR (thermogravimetrical reactor) on the basis of NiO/YSZ particle. From the viewpoints of both reactivity and resistance against carbon deposition, the particle of NiO mixed with YSZ (i.e., yttria-stabilized zirconia) was found to be a good candidate for chemical-looping combustion. We have observed that carbon deposition could be completely avoided with very low concentration of water vapor. By means of a proposed model, the condition that carbon deposition would be avoided was identified.
Utilization of chemokine receptors, orphan receptors, and herpesvirus-encoded receptors by diverse human and simian immunodeficiency viruses (1997)

Human immunodeficiency virus type 1 (HIV-1) requires both CD4 and a coreceptor to infect cells. Macrophage-tropic (M-tropic) HIV-1 strains utilize the chemokine receptor CCR5 in conjunction with CD4 to infect cells, while T-cell-tropic (T-tropic) strains generally utilize CXCR4 as a coreceptor. Some viruses can use both CCR5 and CXCR4 for virus entry (i.e., are dual-tropic), while other chemokine receptors can be used by a subset of virus strains. Due to the genetic diversity of HIV-1, HIV-2, and simian immunodeficiency virus (SIV) and the potential for chemokine receptors other than CCR5 or CXCR4 to influence viral pathogenesis, we tested a panel of 28 HIV-1, HIV-2, and SIV envelope (Env) proteins for the ability to utilize chemokine receptors, orphan receptors, and herpesvirus-encoded chemokine receptor homologs by membrane fusion and virus infection assays. While all Env proteins used either CCR5 or CXCR4 or both, several also used CCR3. Use of CCR3 was strongly dependent on its surface expression levels, with a larger number of viral Env proteins being able to utilize this coreceptor at the higher levels of surface expression. ChemR1, an orphan receptor recently shown to bind the CC chemokine I309 (and therefore renamed CCR8), was expressed in monocyte and lymphocyte cell populations and functioned as a coreceptor for diverse HIV-1, HIV-2, and SIV Env proteins. Use of ChemR1/CCR8 by SIV strains was dependent in part on V3 loop sequences. The orphan receptor V28 supported Env-mediated cell-cell fusion by four T- or dual-tropic HIV-1 and HIV-2 strains. Three additional orphan receptors failed to function for any of the 28 Env proteins tested. Likewise, five of six seven-transmembrane-domain receptors encoded by herpesviruses did not support Env-mediated membrane fusion. However, the chemokine receptor US28, encoded by cytomegalovirus, did support inefficient infection by two HIV-1 strains. These findings indicate that additional chemokine receptors can function as HIV and SIV coreceptors and that surface expression levels can strongly influence coreceptor use.
Low threshold gain metal coated laser nanoresonators (2008)

We introduce a low refractive index layer between the metal and the gain medium in metal-coated laser resonators and demonstrate that it can significantly reduce the dissipation losses. Analysis of a gain medium waveguide shows that for a given waveguide radius, the low index layer has an optimal thickness for which the lasing threshold gain is minimal. The waveguide analysis is used for the design of a novel three-dimensional cylindrical resonator that is smaller than the vacuum wavelength in all three dimensions and exhibits a low enough threshold gain to lase at room temperature.
Centric phase‐encoding order in three‐dimensional MP‐RAGE sequences: Application to abdominal imaging (1992)

Three-dimensional (3D) magnetization-prepared rapid gradient-echo imaging has been proposed as a method for improving signal-to-noise ratio (S/N) and contrast-to-noise ratio (C/N) in rapid abdominal imaging. Originally, a standard sequential phase-encoding order was proposed. In the present study, two approaches to a 3D centric phase-encoding order are presented: (a) application of the two-dimensional (2D) centric order to one of the 3D encoding directions, and (b) an interleaved square spiral order, which is the segmented 3D analog of the 2D centric order. With use of simulation, phantom, and volunteer results, the proposed 3D centric methods are compared in terms of S/N, C/N, and artifacts to the 3D sequential method and 2D magnetization-prepared methods. The second centric approach was found to be superior to the first; however, in general, the 3D technique was found to be inferior to the 2D technique for abdominal imaging because of motion artifact in the 3D image set caused by misregistration among the multiple breath holds required.
Biocompatible Charged and Uncharged Surfaces Using Nanoparticle Films (2010)

Biocompatible surfaces: Cationic, anionic, and neutral nanoparticles (NPs) were immobilized via dithiocarbamate formation onto polyethylenimine (PEI) films to create biofouling resistant surfaces. These surfaces are designed to bind proteins without denaturation, hence preventing biofouling. Extended incubation of these surfaces with BSA and serum demonstrate minimal fouling, providing a new methodology for generating surfaces resistant towards protein fouling.
Interaction of monoclonal antibodies directed against bromodeoxyuridine with pyrimidine bases, nucleosides, and DNA. (1986)

Although antibodies directed against bromodeoxyuridine (BrdU) are being used in both clinical and basic research laboratories as tools to study and monitor DNA synthesis, little is known about the epitopes with which they react. Four monoclonal antibodies directed against BrdU were produced and were characterized to learn more about the epitopes on BrdU which are important for antibody recognition, to identify compounds other than BrdU which react with the antibodies and which might interfere with immunologic assays for BrdU, and to characterize the reaction of these antibodies with BrdU-containing DNA. By radioimmunoassays, the antibodies generally reacted well with 5-iododeoxyuridine, 5-fluorodeoxyuridine, and 5-nitrouracil. However, none of the antibodies reacted well with uridine--indicating that a substituent on uridine C5 was essential for antibody reactivity--or with 5-bromo- or iodo-cytosine, indicating that the region around pyrimidine C4 is important for antibody recognition. Although the antibodies reacted with 5-halogen-substituted uracil bases, the antibodies reacted much better with the corresponding halogenated nucleosides, indicating that the sugar moiety was important for recognition. The presence of a triphosphate group on C'5 of BrdU (i.e., BrdUTP) did not detectably alter antibody recognition. Three of the antibodies reacted only with purified DNA containing BrdU, whereas one antibody, which exhibited a weak interaction with thymidine, also reacted with BrdU-free DNA. S1 nuclease treatment of purified DNA suggested that all four monoclonal antibodies reacted exclusively with single-stranded regions of BrdU-containing DNA. Comparison of detecting DNA synthesis by [3H]TdR incorporation followed by autoradiography with that by BrdU incorporation followed by indirect immunofluorescence indicated that the latter technique was both an accurate and a sensitive measure of DNA synthesis.
Switched-capacitor filters (1990)

A novel technique for building active filters using switched-capacitor (SC) circuits is proposed. Principles of operation, methods of control, analysis, and design of a typical SC filter are presented. To assess its effectiveness, the technique is used to control input current harmonics in a phase-controlled converter. It is shown that a wide range of harmonics can be controlled using a single filter.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
An Ultra-Wide Band System with Chirp Spread Spectrum Transmission Technique (2006)

We consider a wireless Ultra-Wide Band (UWB) communication system using chirp spread spectrum (CSS) transmission technique. A system with high order modulation CSS technique is proposed. Our proposed system has inherited the advantages of CSS technique and common spread spectrum systems. It is immune to Doppler frequency offset and able to increase bit rate by the overlap technique. Existing CSS systems suffer intersymbol interference and high envelope variation caused by the overlap technique seriously while our proposed approach is able to overcome the above disadvantages without loss of the bit rate
Cross-layer scheduling for power efficiency in wireless sensor networks (2004)

Wireless sensor networks are considered the sensing technology of the future. Large numbers of untethered sensor nodes can be used for tracking small animals and targets, environmental monitoring, enforcing security perimeters, etc. A major problem for many sensor network applications is determining the most efficient way of conserving the energy of the power source. Some networks use batteries, while others suggest different methods of gathering energy (e.g., solar cells). Regardless of the powering method, energy conservation is of prime importance for sensor networks. The best way to conserve energy is to turn the sensor nodes off; however, since an inactive sensor node is no longer part of the network, the network can become disconnected. This creates a fundamental trade-off. In this paper, we propose a deterministic, schedule-based energy conservation scheme. In the proposed approach, time-synchronized sensors form on-off schedules that enable the sensors to be awake only when necessary. The schedule establishment is fully distributed and thus appropriate for large sensor networks. The performance of the proposed approach is evaluated through the use of simulations
Crystallization of Methyl Ammonium Lead Halide Perovskites: Implications for Photovoltaic Applications (2014)

Hybrid organic/lead halide perovskites are promising materials for solar cell fabrication, resulting in efficiencies up to 18%. The most commonly studied perovskites are CH3NH3PbI3 and CH3NH3PbI3-xClx where x is small. Importantly, in the latter system, the presence of chloride ion source in the starting solutions used for the perovskite deposition results in a strong increase in the overall charge diffusion length. In this work we investigate the crystallization parameters relevant to fabrication of perovskite materials based on CH3NH3PbI3 and CH3NH3PbBr3. We find that the addition of PbCl2 to the solutions used in the perovskite synthesis has a remarkable effect on the end product, because PbCl2 nanocrystals are present during the fabrication process, acting as heterogeneous nucleation sites for the formation of perovskite crystals in solution. We base this conclusion on SEM studies, synthesis of perovskite single crystals, and on cryo-TEM imaging of the frozen mother liquid. Our studies also included the effect of different substrates and substrate temperatures on the perovskite nucleation efficiency. In view of our findings, we optimized the procedures for solar cells based on lead bromide perovskite, resulting in 5.4% efficiency and Voc of 1.24 V, improving the performance in this class of devices. Insights gained from understanding the hybrid perovskite crystallization process can aid in rational design of the polycrystalline absorber films, leading to their enhanced performance.