gebhart/patrepeval huggingface dataset:
Synthesis and Binding Properties of Oligonucleotides Carrying Nuclear Localization Sequences (1999)

The synthesis of oligonucleotides carrying nuclear localization peptide sequences is described using two strategies: first, oligonucleotides carrying a thiol group at the 5' end were reacted with maleimido peptides; second, peptide and oligonucleotide were prepared stepwise on the same support, yielding oligonucleotide-3'-peptide conjugates. This second approach was thoroughly studied. Using amino acids and small peptides as model compounds, some side reactions were analyzed, detected, and minimized. Oligonucleotides complementary to Ha-ras gene and carrying nuclear localization peptides at the 3' and 5' ends were prepared. Melting temperature studies showed that duplexes containing nuclear localization peptides were more stable than duplexes with unmodified oligonucleotides. Moreover, oligonucleotide-peptide conjugates maintain a good mismatch discrimination when they bind to their target RNA.
Synthesis of 1,3-Dicarbonyl Compounds by the Oxidation of 3-Hydroxycarbonyl Compounds with Corey-Kim Reagent (1988)

A new method for the preparation of various 1,3-dicarbonyl compounds is described. Oxidation of 3-hydroxycarbonyl compounds without substituent at C-2 position by the Corey-Kim reagent (N-chlorosuccinimide-dimethyl sulfide) afforded the stable dimethylsulfonium methylides, which on reductive desulfurization by zinc-acetic acid furnished the 1,3-dicarbonyl derivatives.On the other hand, the same treatment of 2-mono-, or 2,2-disubstituted 3-hydroxy-carbonyl compounds gave directly the corresponding 1,3-dicarbonyl analogous,respectively.
Characterization of cytosolic malic enzyme in human tumor cells (1994)

Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor cells were identical. Thus, NADP(+)-ME found in tumor cells does not constitute a tumor-specific isoform as suggested by other studies but is identical to the 'housekeeping protein' predominantly expressed in human liver and white adipose tissue.
Design and Measurement of a CT $\Delta\Sigma$ ADC With Switched-Capacitor Switched-Resistor Feedback (2009)

The performance of traditional continuous-time (CT) delta-sigma (DeltaSigma) analog-to-digital converters (ADCs) is limited by their large sensitivity to feedback pulse-width variations caused by clock jitter in their feedback digital-to-analog converters (DACs). To mitigate that effect, we propose a modified switched-capacitor (SC) feedback DAC technique, with a variable switched series resistor (SR). The architecture has the additional benefit of reducing the typically high SC DAC output peak currents, resulting in reduced slew-rate requirements for the loop-filter integrators. A theoretical investigation is carried out which provides new insight into the synthesis of switched-capacitor with switched series resistor (SCSR) DACs with a specified reduction of the pulse-width jitter sensitivity and minimal power consumption and complexity. To demonstrate the concept and to verify the reduced pulse-width jitter sensitivity a 5 mW, 312 MHz, second order, low-pass, 1-bit, CT DeltaSigma modulator with SCSR feedback was implemented in a 1.2 V, 90 nm, RF-CMOS process. An SNR of 66.4 dB and an SNDR of 62.4 dB were measured in a 1.92 MHz bandwidth. The sensitivity to wideband clock phase noise was reduced by 30 dB compared to a traditional switched-current (SI) return-to-zero (RZ) DAC.
Three new β,γ-methylene analogues of adenosine triphosphate (1981)

G. M. Blackburn, D. E. Kent and F. Kolkmann, J. Chem. Soc., Chem. Commun., 1981, 1188 DOI: 10.1039/C39810001188
Synthesis and Pyrolysis of Novel Polymeric Precursors to TiC/Al2O3, TiN/Al2O3, and AlN/TiN Nanocomposites (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleSynthesis and Pyrolysis of Novel Polymeric Precursors to TiC/Al2O3, TiN/Al2O3, and AlN/TiN NanocompositesZhiping Jiang and Wendell E. RhineCite this: Chem. Mater. 1994, 6, 7, 1080–1086Publication Date (Print):July 1, 1994Publication History Published online1 May 2002Published inissue 1 July 1994https://pubs.acs.org/doi/10.1021/cm00043a033https://doi.org/10.1021/cm00043a033research-articleACS PublicationsRequest reuse permissionsArticle Views201Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Multiple metal-carbon bonds. 44. Isolation and characterization of the first simple tantalacyclobutane complexes (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleMultiple metal-carbon bonds. 44. Isolation and characterization of the first simple tantalacyclobutane complexesKevin C. Wallace, John C. Dewan, and Richard R. SchrockCite this: Organometallics 1986, 5, 10, 2162–2164Publication Date (Print):October 1, 1986Publication History Published online1 May 2002Published inissue 1 October 1986https://pubs.acs.org/doi/10.1021/om00141a045https://doi.org/10.1021/om00141a045research-articleACS PublicationsRequest reuse permissionsArticle Views169Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Quantum Tunneling of Magnetization and Related Phenomena in Molecular Materials (2003)

Molecules comprising a large number of coupled paramagnetic centers are attracting much interest because they may show properties which are intermediate between those of simple paramagnets and classical bulk magnets and provide unambiguous evidence of quantum size effects in magnets. To date, two cluster families, usually referred to as Mn12 and Fe8, have been used to test theories. However, it is reasonable to predict that other classes of molecules will be discovered which have similar or superior properties. To do this it is necessary that synthetic chemists have a good understanding of the correlation between the structure and properties of the molecules, for this it is necessary that concepts such as quantum tunneling, quantum coherence, quantum oscillations are understood. The goal of this article is to review the fundamental concepts needed to understand quantum size effects in molecular magnets and to critically report what has been done in the field to date.
MU-Tomo: Independent Dose Validation Software for Helical TomoTherapy (2010)

A software program, MU-Tomo, has been developed to perform an independent point dose calculation and compare it to the dose calculated from the TomoTherapy (TomoTherapy, Inc., Madison, WI) treatment planning system (TPS). Input parameters required for this software include: archived tomotherapy patient files, QA plan image coordinates, tomotherapy-calculated point dose and machine-specific dosimetric parameters such as the off-axis ratios (OARx and OARy), tissue phantom ratios (TPR) and output functions (Scp). The software was validated on four phantom models and fifty tomotherapy patient plans representing various anatomical sites. Our results indicate that MU-Tomo can perform in a few seconds an independent dose calculation accurately and provide a secondary check for a point dose validation of helical tomotherapy plans.
Growth of high-quality GaAs/AlAs Bragg mirrors on patterned InP-based quantum well mesa structures (1997)

We have investigated the regrowth of GaAs/AlAs quarter-wave Bragg reflectors on patterned mesa InP-based quantum well heterostructures that can be fabricated into 1.55 μm vertical cavity surface emitting lasers. It is seen from transmission electron and scanning electron microscopy that the multiple layer GaAs-based mirrors can be grown on InP-based heterostructure mesas of diameters 10–40 μm without noticeable propagation of defects into the reflector layers or the quantum well region below. At the same time the photoluminescence from the quantum wells after regrowth indicates that lasers can be fabricated.
Anti-Interleukin-10R1 Monoclonal Antibody Enhances Bacillus Calmette-Guérin Induced T-Helper Type 1 Immune Responses and Antitumor Immunity in a Mouse Orthotopic Model of Bladder Cancer (2012)

Anti-interleukin-10 receptor 1 mAb enhanced the bacillus Calmette-Guérin induced T-helper type 1 immune response and anti-bladder cancer immunity. A humanized form of this mAb warrants future investigation for bacillus Calmette-Guérin treatment of bladder cancer.
<i>In situ</i> passivation and blue luminescence of silicon clusters using a cluster beam/H2O codeposition production method (2009)

Si clusters are produced in a gas aggregation source and fly through ultrahigh vacuum onto a cold target where they are codeposited with water vapor. Melting of the ice yields immediately a suspension of nanoparticles that emits intense, nondegrading luminescence in the blue wavelength range. Spectroscopic analysis reveals a Si/SiO core-shell structure where the luminescence stems from oxygen deficient defects. The main advantage of our production method is that it yields the luminescent Si nanoparticles in one step.
Associate Editor: D. Shugar The 90-kDa Molecular Chaperone Family: Structure, Function, and Clinical Applications. A Comprehensive Review (1998)

The 90-kDa molecular chaperone family (which comprises, among other proteins, the 90- kDa heat-shock protein, hsp90 and the 94-kDa glucose-regulated protein, grp94, major molecular chaperones of the cytosol and of the endoplasmic reticulum, respectively) has become an increasingly active subject of research in the past couple of years. These ubiquitous, well-conserved proteins account for 1-2% of all cellular proteins in most cells. However, their precise function is still far from being elucidated. Their involvement in the aetiology of several autoimmune diseases, in various infections, in recognition of malignant cells, and in antigen-presentation already demonstrates the essential role they likely will play in clinical practice of the next decade. The present review summarizes our current knowledge about the cellular functions, expression, and clinical implications of the 90-kDa molecular chaperone family and some approaches for future research. pharmacol. ther. 79(2):129-168, 1998. © 1998 Elsevier Science Inc.
Angiogenic role for glycodelin in tumorigenesis (2001)

Angiogenesis plays an important role in neovascularization in tumors. Glycodelin, a hormone-responsive protein, has been detected in tumors of reproductive organs and is found in high levels in the plasma of subjects with gynecological malignancies. Glycodelin is also found in the endothelial cells of the umbilical cord and in the blood vessels of tumors. In this study, we tested whether glycodelin-rich amniotic fluid and a synthetic peptide derived from the sequence of glycodelin peptide (Gp) might promote angiogenic response by examining the migration and tube formation in human umbilical cord vein endothelial cells (HUVECs). Increased migration and tube formation of HUVECs were found in the presence of amniotic fluid and Gp, and this increase was blocked by antibody to Gp and by an anti-vascular endothelial growth factor (VEGF) antibody, suggesting that the angiogenic effects of glycodelin might be mediated by VEGF. The results also showed that Gp significantly increased the release of VEGF protein and mRNA expression in HUVECs, RL-95 (human endometrial carcinoma cells), OVCAR-3 (human ovarian adenocarcinoma cells), EM42 (human endometrial epithelial cells), THP-1 (human monocyte), and MCF-7 and MDA-MB-231 (human breast adenocarcinoma cells) cell lines. VEGF receptor Fit-1 mRNA expression in HUVECs was also increased in the presence of Gp. These findings, together with the suggestion from the literature that glycodelin may have immunosuppressive properties, suggest that glycodelin might play an important role in neovascularization during embryogenesis and tumor development.
Photomagnetic Anneal, A New Magneto-Optic Effect, in Si-Doped Yttrium Iron Garnet (1967)

Experimental evidence is given to show that the magnetocrystalline anistropy of silicondoped yttrium iron garnet, nominal composition ${\mathrm{Y}}_{3}$${\mathrm{Fe}}_{4.9}$${\mathrm{Si}}_{0.1}$${\mathrm{O}}_{12}$, can be changed by more than 100 Oe at 20\ifmmode^\circ\else\textdegree\fi{}K through irradiation with near-infrared photons. The change is interpreted as due to the redistribution of electrons between iron ions, that is valence exchange.
Spinal Cord Electrical Stimulation in Severe Angina Pectoris: Surgical Technique, Intraoperative Physiology, Complications, and Side Effects (1989)

Twenty patients with angina pectoris were treated with spinal cord stimulation (SCS) at the T1,T2 level of the spinal cord since June 1985. These patients were not candidates for angioplasty or coronary bypass or those procedures had failed. There were no injections. One lead broke and one lead migrated. Both were corrected migrally and regained some relief. The relief of pain with SCS may be an alternative treatment to coronary bypass or angioplasty in some patients.
In a concerted action kit ligand and interleukin 3 control the synthesis of serotonin in murine bone marrow-derived mast cells. Up-regulation of GTP cyclohydrolase I and tryptophan 5-monooxygenase activity by the kit ligand (1993)

Mouse bone marrow-derived mast cells (BMMC) store and release serotonin whose synthesis is initiated by tryptophan 5-monooxygenase. (6R)-H4biopterin serves as the natural cofactor for this reaction. GTP cyclohydrolase I catalyzes the first and rate-limiting step of its synthesis. In this study we demonstrate that among a panel of growth-promoting cytokines including kit ligand (KL), interleukin 3 (IL-3), IL-4, IL-9, and nerve growth factor, KL selectively enhances the synthesis of H4biopterin through up-regulation of GTP cyclohydrolase I activity to 6.2-fold levels. The activities of the subsequent enzymes 6-pyruvoyl-H4pterin synthase and sepiapterin reductase remain unaffected. The activity of tryptophan 5-monooxygenase was selectively enhanced 4.5-fold by the combination of IL-3 with KL. All other factors could not substitute for KL. The constitutive high activity of aromatic L-amino acid decarboxylase is not different in cells cultured in IL-3 and/or KL. In consequence, the concerted action of IL-3 and KL on the GTP cyclohydrolase I and the tryptophan 5-monooxygenase reaction enhances the production of serotonin to about 20-fold levels. Additionally, KL specifically causes the release of about half of total serotonin produced. Hence, our data demonstrate a novel role of these cytokines for the function of mouse BMMC and provide a coherent view of the regulation of serotonin synthesis in this cell type.
The complete amino acid sequence of the <i>Clostridium botulinum</i> type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene (1990)

A 26-mer oligonucleotide probe was synthesized (based on the determined amino acid sequence of the N-terminus of the Clostridium botulinum type A neurotoxin, BoNT/A) and used in Southern blot analysis to construct a restriction map of the region of the clostridial genome encompassing BoNT/A. The detailed information obtained enabled the cloning of the structural gene as three distinct fragments, none of which were capable of directing the expression of a toxic molecule. The central portion was cloned as a 2-kb PvuII-TaqI fragment and the remaining regions of the light chain and heavy chain as a 2.4-kb ScaI-TaqI fragment and a 3.4-kb HpaI-PvuII fragment, respectively. The nucleotide sequence of all three fragments was determined and an open reading frame identified, composed of 1296 codons corresponding to a polypeptide of 149 502 Da. The deduced amino acid sequence exhibited 33% similarity to tetanus toxin, with the most highly conserved regions occurring between the N-termini of the respective heavy chains. Conservation of Cys residues flanking the position at which the toxins are cleaved to yield the heavy chain and light chain allowed the tentative identification of those residues which probably form the disulphide bridges linking the two toxin subfragments.
A Machine Learning based Efficient Software Reusability Prediction Model for Java Based Object Oriented Software (2014)

Software reuse refers to the development of new software systems with the likelihood of completely or partially using existing components or resources with or without modification. Reusability is the measure of the ease with which previously acquired concepts and objects can be used in new contexts. It is a promising strategy for improvements in software quality, productivity and maintainability as it provides for cost effective, reliable (with the consideration that prior testing and use has eliminated bugs) and accelerated (reduced time to market) development of the software products. In this paper we present an efficient automation model for the identification and evaluation of reusable software components to measure the reusability levels (high, medium or low) of procedure oriented Java based (object oriented) software systems. The presented model uses a metric framework for the functional analysis of the Object oriented software components that target essential attributes of reusability analysis also taking into consideration Maintainability Index to account for partial reuse. Further machine learning algorithm LMNN is explored to establish relationships between the functional attributes. The model works at functional level rather than at structural level. The system is implemented as a tool in Java and the performance of the automation tool developed is recorded using criteria like precision, recall, accuracy and error rate. The results gathered indicate that the model can be effectively used as an efficient, accurate, fast and economic model for the identification of procedure based reusable components from the existing inventory of software resources.
A systems-level modular design approach to field robotics (2002)

Long development times and high costs prevent robots from being practical for use in many important field missions. Here a modular design approach is proposed to produce a rapidly deployable low cost field robotic system. An inventory of components such as actuated joints, links, power supplies, and software modules are assembled to produce a field robotic system for a specified task. The proposed design method uses a hierarchical selection process combined with a genetic algorithm to select a robot configuration and action plan for a given task. This method is applied to an inspection task required for the preservation of the USS Constitution, an historic naval ship.
Rho/ROCK and MAPK signaling pathways are involved in glioblastoma cell migration and proliferation. (2009)

Rho/ROCK signaling is involved in GBM cell migration and proliferation, and this pathway may be linked to ERK signaling.
Differential surface labeling and internalization of glucagon by peripheral leukocytes. (1983)

Free accessAbstractFirst published March 1983Differential surface labeling and internalization of glucagon by peripheral leukocytes.G A Ackerman, J Yang, and K W WolkenView all authors and affiliationsVolume 31, Issue 3https://doi.org/10.1177/31.3.6186734
Eicosapentaenoic Acid and Docosahexaenoic Acid Block Serotonin-Induced Smooth Muscle Cell Proliferation (1999)

Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) present in fish oils have been ascribed as having significant antithrombotic and antiatherosclerotic effects. Vascular smooth muscle cell (SMC) proliferation plays an important role in the pathogenesis of atherosclerosis and restenosis. Recent studies have indicated that serotonin at concentrations present at sites of vascular injury stimulates SMC proliferation and may contribute to the restenotic process. In the present study we demonstrate that among the fatty acids tested, only EPA and DHA could block the mitogenic effect of serotonin on vascular SMC. Further, when added together these fatty acids act synergistically in blocking the mitogenic effect of serotonin. EPA and DHA blocked the 5HT-induced increase in the 5-HT(2) receptor mRNA. This antimitogenic effect of EPA and DHA may partially explain some of the beneficial effects of fish oils.
Stereoscopic camera system with creator-friendly functions (2009)

Stereoscopic filming is roughly divided into two types: toed-in and parallel camera configurations. Both types have disadvantages: toed-in cameras cause keystone distortions, and parallel cameras cause image loss by shifting. In addition, it is difficult for inexperienced creators to understand the optimal camera settings and post-processing procedures, such as cross points and inter-camera distance, in both types. These factors hinder the creation of stereoscopic images. Therefore, the authors focused on improving usability in stereoscopic filming, constructed an experimental camera system, and examined semi-automatic camera configuration function in terms of viewing safety.
Mouse Morphological Phenotyping With Magnetic Resonance Imaging (2006)

The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, with both MRI physicists and biologists starting to use MRI to identify mouse models of human disease. The purpose of this chapter is to provide details of the animal handling necessary for routine and robust in vivo imaging with particular emphasis on multiple-mouse imaging. In addition, techniques for perfusion-fixation for postmortem imaging of specimens and whole mice are given.