gebhart/patrepeval huggingface dataset:
The MN1-TEL Fusion Protein, Encoded by the Translocation (12;22)(p13;q11) in Myeloid Leukemia, Is a Transcription Factor with Transforming Activity (2000)

The Tel gene (or ETV6) is the target of the translocation (12;22)(p13;q11) in myeloid leukemia. TEL is a member of the ETS family of transcription factors and contains the pointed protein interaction (PNT) domain and an ETS DNA binding domain (DBD). By contrast to other chimeric proteins that contain TEL's PNT domain, such as TEL-platelet-derived growth factor beta receptor in t(5;12)(q33;p13), MN1-TEL contains the DBD of TEL. The N-terminal MN1 moiety is rich in proline residues and contains two polyglutamine stretches, suggesting that MN1-TEL may act as a deregulated transcription factor. We now show that MN1-TEL type I, unlike TEL and MN1, transforms NIH 3T3 cells. The transforming potential depends on both N-terminal MN1 sequences and a functional TEL DBD. Furthermore, we demonstrate that MN1 has transcription activity and that MN1-TEL acts as a chimeric transcription factor on the Moloney sarcoma virus long terminal repeat and a synthetic promoter containing TEL binding sites. The transactivating capacity of MN1-TEL depended on both the DBD of TEL and sequences in MN1. MN1-TEL contributes to leukemogenesis by a mechanism distinct from that of other chimeric proteins containing TEL.
Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody. (1980)

We recently reported the isolation of a rat monoclonal antibody designated 2.4G2 (9) that is directed against the mouse trypsin-resistant Fc receptor (FcR) for IgG2b and IgG1 immune aggregates. We have now utilized the Fab fragment of 2.4G2 as an affinity reagent to purify FcR from the macrophage cell line J774 to apparent homogeneity. The antigen isolated from J774 cells consisted of two general types of polypeptides with broad electrophoretic mobilities of approximately 60,000 and 47,000 mol wt. Similar broad bands ranging from 47,000 to 70,000 mol wt were isolated from various FcR-bearing cell lines of B, T, and null lymphocyte, as well as of macrophage origin. J774 FcR was judged to be a glycoprotein based on the sensitivity of its isoelectric point to neuraminidase digestion, its labeling with galactose oxidase/NaB[3H4], and its binding to concanavalin A-Sepharose. In phosphate-buffered saline, the isolated protein formed large aggregates that were shown to retain FcR activity, albeit with a somewhat altered IgG subclass specificity. The FcR aglutinated erythrocytes that were coated with both IgG2b and IgG2a that did not otherwise hemagglutinate. In addition, iodinated FcR bound to Sephadex beads coated with rabbit IgG, mouse IgG1, IgG2b, and IgG2a, but not to beads coated with mouse IgG3 or rabbit F(ab')2 fragments. The binding of the purified receptor to all IgG classes was inhibited by the Fab fragments of 2.4G2. In contrast, the binding of IgG2a to intact macrophages was inhibited by 2.4G2 Fab by only 15%, whereas rabbit IgG immune aggregate binding was almost completely abolished.
Reciprocal circular polarization selective surfaces (1996)

nationale du Canada de reproduire, prter, distribuer ou vendre des copies de sa thse de quelque manire et sous quelque forme que ce soit Pour mettre des exemplaires de cette thse la disposition des personnes intresses.L'auteur conserve la proPrit du droit d'auteur qui Protge sa thse.Ni la thse ni des extraits substantiels de celle'ci ne doivent tre imprims ou autrement reproduits sans son autorisation.
Metabolites of Aspergillus ustus. Part 4. Stable-isotope labelling studies on the biosynthesis of the austalides (1987)

Incorporationof [1-13C]-, [1,2-13C2]-and [1-13C,2,2,2-2H3]-acetate, and (3RS)-[2-13C]mevalonolactone into austalide D(2), a metabolite of Aspergillus ustus, shows that the austalides are derived from 6[(2E,6E)farnesyl]-5,7-dihydroxy-4-methylphthalide. The proposed mechanism for the subsequent cyclisation and oxidative modifications of the farnesyl moiety, consistent with the relative stereochemistry of the austalides and the structures of the cometabolites, austalides J (4), K (5), and L (6), is supported by the incorporation of austalide K into austalide D. The addition of ethanol to growing cultures of A. ustus severely inhibits normal metabolite production and induces the formation of a new metabolite, ethyl 6-ethyl-2,4-dihydroxy-3-methylbenzoate (11). The biosynthetic origin of this metabolite was studied using [1-13C]- and [1-13C,2,2,2-2H3]acetate and (2S)-[methyl-13C]methionine as precursors.
Heterologous expression of an engineered truncated form of human Lewis fucosyltransferase (Fuc-TIII) by the methylotrophic yeast Pichia pastoris (1998)

A stable GS115 Pichia pastoris recombinant strain was constructed to secrete a truncated form of the human alpha(1,3/4) fucosyltransferase (amino acids 45-361). Enzyme production resulted from a secretory pathway based on the pre-pro- alpha mating factor signal sequence of the yeast Saccharomyces cerevisiae . Following its transit through the Golgi apparatus, the enzyme accumulated in the periplasmic space before its release in the culture broth (about 30 mg/l). Cell-enclosed enzyme ( approximately 0.16%) proved to be fairly stable for many freezing and thawing cycles and could be used several times as an immobilized catalyst. Soluble enzyme (>99.8%) representing the main protein of the culture broth (10%) has been characterized by Western-blotting, substrate specificities and kinetic parameters. The two forms (cell-enclosed and soluble) of recombinant enzyme may be used for in vitro synthesis of Lewisadeterminants.
Modification of Tip Vortex by Spanwise Slots (1999)

Characteristics of trailing vortices generated by a rectangular fixed wing with the NACA23012 airfoil section have been investigated with a 2-dimensional LDV system. Curved slots were imbedded near the rectangular tip to blow a slight amount of the main stream into the tip face and to modify tip vortices. Tangential and axial velocity components of tip vortices, circulations and momentum deficits were measured at vertical planes to the vortex trail for both the plain and the slotted wings, respectively. Lift distributions over both wings were also investigated by the use of the bound dirculations measured at five different locations for each span. It was found that the slotted wing develops a fairing effect at the tip and lessens the vortex strengths without serious defects for the lift losses even though the slight momentum deficit was perceived for this selected model. It was, therefore, confirmed that the slotted wing can be used for a device to improve the aerodynamic performance by the tip vortex modification.
Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics (2014)

New therapeutic strategies are needed to treat infections caused by drug-resistant bacteria, which constitute a major growing threat to human health. Here, we use a high-throughput technology to identify combinatorial genetic perturbations that can enhance the killing of drug-resistant bacteria with antibiotic treatment. This strategy, Combinatorial Genetics En Masse (CombiGEM), enables the rapid generation of high-order barcoded combinations of genetic elements for high-throughput multiplexed characterization based on next-generation sequencing. We created ∼ 34,000 pairwise combinations of Escherichia coli transcription factor (TF) overexpression constructs. Using Illumina sequencing, we identified diverse perturbations in antibiotic-resistance phenotypes against carbapenem-resistant Enterobacteriaceae. Specifically, we found multiple TF combinations that potentiated antibiotic killing by up to 10(6)-fold and delivered these combinations via phagemids to increase the killing of highly drug-resistant E. coli harboring New Delhi metallo-beta-lactamase-1. Moreover, we constructed libraries of three-wise combinations of transcription factors with >4 million unique members and demonstrated that these could be tracked via next-generation sequencing. We envision that CombiGEM could be extended to other model organisms, disease models, and phenotypes, where it could accelerate massively parallel combinatorial genetics studies for a broad range of biomedical and biotechnology applications, including the treatment of antibiotic-resistant infections.
An Automatic Calibrating Apparatus for Standard Scales by Counting Interference Fringes (1964)

A new device is described with which the calibration of a standard scale up to 1000 mm long can be made in terms of interference fringes in an hour’s run. A standard scale on a carriage is translated longitudinally with uniform speed of 5 mm/min. Fringes produced by a Michelson interferometer are changed to a train of electric pulses that measures the translation with the unit of a quarter of wavelength used (5461 Å), and these pulses are accumulated on a counter successively. An impulse is produced by a fixed photoelectric microscope at every passage of graduation lines across the microscope axis. At every moment when the impulse comes from the microscope, the immediate content of the counter is taken out and printed automatically. A maximum of six photoelectric microscopes are used in tandem to extend the limited coherence length, and the batches of data are shown to be connected without introducing any appreciable errors. Thus a single run over 300 mm has made it possible to calibrate all graduations with a total accuracy of ±0.1 μ.
Understanding the control of <i>Pseudomonas aeruginosa</i> alginate synthesis and the prospects for management of chronic infections in cystic fibrosis (2005)

Decades of research have been dedicated to the study of the opportunistic pathogen Pseudomonas aeruginosa, a Gram-negative, environmental bacterium that secretes the exopolysaccharide alginate during chronic lung infection of cystic fibrosis (CF) patients. Although P. aeruginosa utilizes a variety of factors to establish a successful infection in the lungs of CF patients, alginate has stood out as one of the best-studied prognostic indicators of chronic lung infection. While the genetics, biosynthesis and regulation of alginate are well understood, questions still remain concerning its role in biofilm development and its potential as a therapeutic target. The purpose of this review is to provide a brief summary of alginate biosynthesis and regulation, and to highlight recent discoveries in the areas of alginate production, biofilm formation and vaccine design. This information is placed in context with a proposed P. aeruginosa infectious pathway, highlighting avenues for the use of existing therapies as well as the potential for novel agents to reduce or eliminate chronic infections in CF patients.
Public-key encryption based on Chebyshev maps (2003)

We propose a public-key encryption algorithm based on Chebyshev maps, which is secure, practical, and can be used for both encryption and digital signature. Software implementation and properties of the algorithm are discussed in detail.
GRAD: On Graph Database Modeling (2016)

Graph databases have emerged as the fundamental technology underpinning trendy application domains where traditional databases are not well-equipped to handle complex graph data. However, current graph databases support basic graph structures and integrity constraints with no standard algebra. In this paper, we introduce GRAD, a native and generic graph database model. GRAD goes beyond traditional graph database models, which support simple graph structures and constraints. Instead, GRAD presents a complete graph database model supporting advanced graph structures, a set of well-defined constraints over these structures and a powerful graph analysis-oriented algebra.
High precision clockless ADC using wavelet neural network (2016)

A design technique for an asynchronous Analog-to-Digital Converter (ADC) is presented. The proposed design retains a clockless level crossing sampling technique, and then applies a Wavelet Neural Network (WNN) technique. High-level simulation results are shown for various ADC resolutions. The Signal to Noise and Distortion Ratio (SNDR) achieved for 4-bit ADC systems are presented. It has been shown that a 4-bit system with the proposed asynchronous ADC architecture using a WNN technique achieves an Effective Number Of Bits (ENOB) of up to 38 bits depending on the input frequency and resolution.
Improved ADPCM voice signal transmission employing click-noise detection scheme for TDMA-TDD personal communication systems (1997)

In personal communication systems, better voice quality and lower terminal price are essential requirements, and adaptive differential pulse-code modulation (ADPCM) codecs are one of the voice codec candidates that have good voice quality and reasonable cost. However, in slow fading environments, burst errors induce click noise in ADPCM voice signal transmission. This paper proposes a novel error-concealment scheme to improve the ADPCM voice signal transmission quality of time division multiple access time division duplex (TDMA-TDD) personal communication systems in slow fading environments. The proposed scheme employs an improved error-detection technique with PCM differential detection and overflow detection coupled with a cyclic redundancy check (CRC) code. To compensate for residual click noise, an expanded CRC error-detection signal is used. Erroneous PCM signals are adaptively suppressed according to the click-noise detection information. Hardware simulation results show that these techniques significantly improve ADPCM voice signal transmission quality by 0.5 points and 1.0 compared to that of the conventional ADPCM muting scheme and no processing, respectively, with the mean opinion scoring (MOS) test in the slow fading environments typical of personal communication systems.
Prophylactic Effect of a<i>Lactobacillus casei</i>Preparation on the Recurrence of Superficial Bladder Cancer (1992)

A randomized controlled study was conducted in patients with superficial bladder cancer in order to investigate the safety of an orally administered (3 g/day) Lactobacillus preparation, biolactis powder (BLP), and its preventive effect on the recurrence after transurethral resection of the bladder tumor (TUR-Bt). Of 58 cases enrolled in the study 23 cases in the BLP group and 25 cases in the control group completed the study. There were no significant differences in the patient characteristics for the complete cases between the two groups. Comparison of the disease-free duration by the Kaplan-Meier method revealed that the 50% recurrence-free interval after TUR-Bt was prolonged by BLP treatment (350 days) to 1.8 times that in the control group (195 days). A significant difference between the groups was detected by the long-rank test (p = 0.03). No adverse side effect was observed. The results suggest that oral administration of BLP is useful for the prevention of the recurrence of superficial bladder cancer.
Using skin-color motion for 3D face modelling and synthesis (2004)

Today 3D human face models have been widely used in applications such as face recognition, head tracking, facial expression recognition, human action recognition, video compression/coding and facial animation. Modeling human faces provides a potential solution to identifying faces with variations in illumination, pose, and facial expression. We propose a method of modeling human faces based on a generic MPEG-4 face model (a triangular mesh model) and individual facial characteristics containing both shape and texture information. The modeling method adapts a generic face model to the extracted facial features.
Targeted disruption of the meprin metalloproteinase β gene protects against renal ischemia-reperfusion injury in mice (2008)

Meprins are membrane-bound and secreted metalloproteinases consisting of alpha- and/or beta-subunits that are highly expressed in mouse kidney proximal tubules. Previous studies have implied that the meprin alpha/beta-isoform is deleterious when renal tissue is subjected to ischemia-reperfusion (I/R). To delineate the roles of the meprin isoforms in renal disease, we subjected mice deficient in meprin-beta (KO) and their wild-type (WT) counterparts to I/R. WT mice were markedly more susceptible to renal injury after I/R than the meprin-beta KO mice as determined by blood urea nitrogen levels. Urinary levels of inflammatory cytokines IL-6 and KC (CXCL1) were significantly higher in WT compared with meprin-beta KO mice by 6 h post-I/R. At 96 h postischemia, kidney mRNA expression levels for tumor necrosis factor-alpha, transforming growth factor-beta, inducible nitric oxide synthase, and heat shock protein-27 were significantly higher in the WT than meprin-beta KO mice. For WT mice subjected to I/R, there was a rapid (3 h) redistribution of meprin beta-subunits in cells in S3 segments of proximal tubules, followed by shedding of apical cell membrane and detachment of cells. These studies indicate that meprin-beta is important in the pathogenesis of renal injury following I/R and that the redistribution of active meprin-alpha/beta is a major contributor to renal injury and subsequent inflammation.
Future Directions: Growth Prediction Models (2007)

The predictive value of these models could be improved by the inclusion of extended anthropometric variables and biological parameters such as insulin-like growth factor I levels. However, recent reports that common polymorphisms of theGH receptor (GHR) gene may be associated with variations in response to GH suggest that, in the future, molecular genetics may provide an additional tool for refining growth prediction models. This possibility is being explored in a pilot study examining the effects of candidate genes in a targeted KIGS population to determine whether the GHR geneor other gene variants contribute to growth response over the first year of GH treatment.
Multiprobe monitoring of ionic, metabolic, and electrical activities in the awake brain (1982)

A new approach for studying brain metabolic, ionic, and electrical activities in the awake animal is described. We developed a multiprobe assembly holding electrodes for extracellular K+, pH, DC potential, electrocorticogram, and temperature as well as a light guide for monitoring intramitochondrial NADH oxidation-reduction state. The assembly was designed so that because of the type of electrodes used and the protection system around them, the same system could be used in many experiments. The results presented here are typical and show the potential use of the multiprobe approach for studying the effects of hypoxia, anoxia, spreading depression, and ischemia on the awake brain. From the results obtained the following conclusions can be drawn. 1) The DC correction for the K+ and H+ measurements is necessary, although it was not as good for the H+ as for the K+ signals. 2) During ischemia (complete or partial) a clear acidification of the brain was found in correlation with the decrease in oxygen availability as evaluated by the NADH fluorescence signal. 3) During brain activation (induced by spreading depression) extracellular K+ was elevated and then actively pumped back into the cells. The NADH showed an oxidation response, and the pH response started with an alkalinization followed by a short acidification.
Circuits using uniform TFTs based on amorphous In‐Ga‐Zn‐O (2007)

Abstract— High‐performance and excellent‐uniformity thin‐film transistors (TFTs) having bottom‐gate structures are fabricated using an amorphous indium‐gallium‐zinc‐oxide (IGZO) film and an amorphous‐silicon dioxide film as the channel layer and the gate insulator layer, respectively. All of the 94 TFTs fabricated with an area 1 cm 2 show almost identical transfer characteristics: the average saturation mobility is 14.6 cm 2 /(V‐sec) with a small standard deviation of 0.11 cm 2 /(V‐sec). A five‐stage ring‐oscillator composed of these TFTs operates at 410 kHz at an input voltage of 18 V. Pixel‐driving circuits based on these TFTs are also fabricated with organic light‐emitting diodes (OLED) which are monolithically integrated on the same substrate. It is demonstrated that light emission from the OLED cells can be switched and modulated by a 120‐Hz ac signal input. Amorphous‐IGZO‐based TFTs are prominent candidates for building blocks of large‐area OLED‐display electronics.
High-speed adder design using time borrowing and early carry propagation (2002)

This paper presents a novel application of skew-tolerant domino circuit design by combining the effects of time borrowing and early carry propagation in a 64-bit adder. Skew-tolerant circuit design softens the clock edges and allows time borrowing from one stage of logic to the next in a pipeline. Early carry propagation also softens clock edges by allowing useful work to be done during the precharge phase. In this paper, these two effects are used simultaneously, resulting in a significant reduction in latency. Simulation results show that up to a 42% decrease in delay can be achieved over a traditional two-phase clocking design when both techniques are combined in the same circuit.
Controllable fabrication of SiGe/Si and SiGe/Si/Cr helical nanobelts (2005)

Helical nanobelts of SiGe/Si and SiGe/Si/Cr are fabricated by rolling up strained thin heterostructures. The fabrication involved electron beam lithography, reactive ion etching, and wet chemical etching steps followed by a drying procedure. All parameters of the helical nanobelts, namely their helical angle, chirality, pitch and diameter, are controllable in a reproducible fashion. The ease of fabrication of SiGe/Si and hybrid helical nanobelts opens new paths for the fabrication technology of micro- or nanoscale sensors, transducers, resonators and cylindrical shaped micro-capacitors.
Cytokine control of parasite-specific anergy in human lymphatic filariasis. Preferential induction of a regulatory T helper type 2 lymphocyte subset. (1993)

The immunological mechanisms involved in maintenance of an asymptomatic microfilaremic state (MF) in patients with lymphatic filariasis remain undefined. MF patients have impaired filarial antigen (Ag)-specific lymphocyte proliferation and decreased frequencies (Fo) of Ag-specific T cells, and yet elevated serum IgE and antifilarial IgG4. To investigate the mechanism of Ag-specific anergy in MF patients in contrast to amicrofilaremic individuals with chronic lymphatic obstruction (CP), the Fo of Ag-specific lymphocytes from peripheral blood mononuclear cells secreting either IL-4 or IFN-gamma were assessed by filter spot enzyme-linked immunosorbent assay, and IL-10 and transforming growth factor-beta (TGF-beta) mRNA transcript levels were assessed by a semiquantitative reverse transcriptase polymerase chain reaction technique. The Fo of filaria-specific IL-4-secreting lymphocytes were equivalent in both MF (geometric mean [GM] = 1:11,700) and CP (GM = 1:29,300 P = 0.08), whereas the Fo of IFN-gamma-secreting lymphocytes were lower in MF (GM = 1:39,300) than in CP (GM = 1:4,200, P < 0.01). When the ratio of IL-4/IFN-gamma (T helper type 2 [Th2]/Th1)-secreting cells was examined, MF subjects showed a predominant Th2 response (8:1) compared with a Th1 response in CP individuals (1:4). mRNA transcript levels of IL-10 were also significantly elevated in MF compared with CP individuals (P < 0.01). Further, IL-10 and TGF-beta were shown to have a role in modulating the Ag-specific anergy among MF subjects, in that neutralizing anti-IL-10 or anti-TGF-beta significantly enhanced lymphocyte proliferation response (by 220-1,300%) to filarial Ags in MF individuals. These findings demonstrate that MF subjects respond to parasite antigen by producing a set of suppressive cytokines that may facilitate persistence of the parasite within humans while producing little clinical disease.