gebhart/patrepeval huggingface dataset:
Effectiveness of Subcutaneous Dihydroergotamine by Home Injection for Migraine (1996)

The effectiveness of dihydroergotamine administered by home subcutaneous injection by the patient or family for severe headache attacks was assessed retrospectively in 51 patients. Average follow-up was 21 weeks. Twenty-one patients had intermittent migraine attacks, 27 had transformed migraine with chronic daily headache, and 3 had chronic tension-type headache. Of the 51 patients taught home injection, 35% had an excellent overall response, 18% had a good response, 12% had a poor response but continued to use dihydroergotamine, and 35% had discontinued dihydroergotamine use. Side effects were the main reason for stopping dihydroergotamine. These included nausea or vomiting or both, limb pain or numbness or both, chest or throat tightness or both, and soreness at the injection site. Thirty-three patients (65%) continued to use dihydroergotamine at the end of the follow-up period. In patients who previously required injections from medical personnel for headache crises and in whom home injection of dihydroergotamine was effective, a dramatic reduction occurred in hospital emergency room and physician office utilization. Dihydroergotamine use by home injection can be an effective treatment for a significant proportion of patients with severe migraine including patients with transformed migraine and medication overuse.
Antibody-Free Magnetic Cell Sorting of Genetically Modified Primary Human CD4+ T Cells by One-Step Streptavidin Affinity Purification (2014)

Existing methods for phenotypic selection of genetically modified mammalian cells suffer disadvantages of time, cost and scalability and, where antibodies are used to bind exogenous cell surface markers for magnetic selection, typically yield cells coated with antibody-antigen complexes and beads. To overcome these limitations we have developed a method termed Antibody-Free Magnetic Cell Sorting in which the 38 amino acid Streptavidin Binding Peptide (SBP) is displayed at the cell surface by the truncated Low Affinity Nerve Growth Receptor (LNGFRF) and used as an affinity tag for one-step selection with streptavidin-conjugated magnetic beads. Cells are released through competition with the naturally occurring vitamin biotin, free of either beads or antibody-antigen complexes and ready for culture or use in downstream applications. Antibody-Free Magnetic Cell Sorting is a rapid, cost-effective, scalable method of magnetic selection applicable to either viral transduction or transient transfection of cell lines or primary cells. We have optimised the system for enrichment of primary human CD4+ T cells expressing shRNAs and exogenous genes of interest to purities of >99%, and used it to isolate cells following Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 genome editing.
965. The preparation and properties of some plutonium compounds. Part VII. Plutonium carbides (1957)

J. L. Drummond, B. J. McDonald, H. M. Ockenden and G. A. Welch, J. Chem. Soc., 1957, 4785 DOI: 10.1039/JR9570004785
Locomotive Asset Management: Integration OfGPS, Mobile Telemetry, And GIS Technologies (1998)

The paper discusses the prototype architecture and capabilities of a Locomotive Condition Monitoring System (LCMS) utilizing an open computing platform, public communications service and commercial software. Integration of locomotive originated data and existing data sets, along with proposed enhancements are also discussed. The treatise documents the use of computer technologies to aid and improve asset management in a railroad environment. Though deployed on a freight railroad, the LCMS application is readily transportable to a rail transit environment and can provide a core platform for the implementation of other applications requiring data from on-board a rail vehicle.
Determination of effective energies in CT calibration (1978)

The effective energy of a polychromatic beam for a Computed Tomography (CT) scanner can be measured directly only with difficulty. However, a linear relationship exists between the measured CT numbers and corresponding attenuation coefficients of known materials at the effective energy of the x-ray beam. The effective energy can then be determined by searching all energies for the best linear correlation between the CT numbers and the attenuation coefficients. This can be performed by two methods: graphically, by means of choosing visually the straightest of the fitted lines or, mathematically, by maximizing the correlation coefficient. The energy corresponding to the optimal fit is therefore selected as the effective energy. The latter method was implemented by computer and demonstrated by scanning the AAPM phantom, which contained known materials, and determining the effective energies and the relationship between the linear attenuation coefficients and CT numbers for three commercial units.
The observation of myofibroblasts in hypertrophic scars. (1975)

Contractile fibroblasts, myofibroblasts, are common cellular components of immature human hypertrophic scars. The contraction of these cells, could play a significant role in the elevation of the scar tissue. The appearance of such cells within granulation tissue suggests that the tissue is pre-disposed to hypertrophy at an early stage of repair.
BindingDB Entry 5375: [Second generation SSRIS: human monoamine transporter binding profile of escitalopram and R-fluoxetine]. (2012)

As noted previously, paroxetine and sertraline possess moderate affinity (<50 nmol/L) for the human norepinephrine transporter and dopamine transporter, respectively. R-fluoxetine, unlike the other selective serotonin reuptake inhibitors, possesses moderate affinity (Ki=64 nmol/L) for the serotonin 2C receptor. Potential clinical correlates of these unique attributes of escitalopram and R-fluoxetine are discussed. (Biol Psychiatry 2001; 50: 345-350 " 2001 Society of Biological Psychiatry).
Inside Microsoft Windows CE (1998)

From the Publisher:
Inside Microsoft Windows CE reveals the innovative system architecture that enables you, the developer or technology champion, to capitalize on new markets in consumer and industrial electronics, including: handheld PCs, palm-sized PCs, computers for automobiles, Web televisions and Web telephones, game consoles, DVD and multimedia devices, and the undisclosed and the uninvented. You'll also find conceptual frameworks to help you understand your design options and see real-world examples that demonstrate the flexibility and potential of this remarkable operating system.
Iron protoporphyrin IX (hemin) but not tin or zinc protoporphyrin IX can stimulate gene expression in K562 cells from enhancer elements containing binding sites for NF-E2 (1994)

Many genes whose transcription is erythroid-specific contain enhancer or promoter elements that bind the transcription factor NF-E2. Hemin induction increases the expression of globin genes in the human erythroleukemia cell line K562, and increases the expression of reporters gene regulated by an enhancer elements containing binding sites for NF-E2. The failure of metalloporphyrins other than hemin to stimulate the transient expression of a CAT reporter gene linked to an enhancer element containing a binding site for NF-E2 was correlated with their failure to induce benzidine-positive K562 cells and increase the steady-state level of gamma-globin mRNA. This study suggests that elevated levels of zinc protoporphyrin IX found in the anemia of chronic disease, iron deficiency, and lead poisoning may contribute to a decrease in globin gene expression by interfering with the transcriptional activity of enhancer elements containing binding sites for NF-E2.
Tunable Lasing from a Cholesteric Liquid Crystal Film Embedded with a Liquid Crystal Nanopore Network (2011)

Continuous tuning of lasing wavelength is achieved in cholesteric liquid crystal lasers by embedding a network of nanopores with an average size of 10 nm filled with liquid crystals inside a polymerized matrix with helical order. The device possesses both high transparency and a fast response time because the tuning is driven by local reorientation of the liquid crystal molecules in the nanopores.
Prevalence of a history of prior varicella/herpes zoster infection in multiple sclerosis (2017)

Varicella zoster virus (VZV) infection has been implicated in multiple sclerosis (MS), but direct causal involvement has been disputed. Nevertheless, knowledge of VZV exposure is important, given the risk of serious complications of first exposure while undergoing immunosuppressive treatment, in particular with fingolimod. We distributed questionnaires to MS clinic patients, requesting information about history of chickenpox, sibling/household/occupational exposure, history of zoster (shingles), and disease-modifying treatment. A random, proportionally representative sample of 51 patients that included patients with positive, negative, and unknown chickenpox history were selected for determination of VZV IgG by ELISA. Of 1206 distributed questionnaires, 605 were returned (50% response rate). Of these, 86% reported history of chickenpox, 5.6% gave negative history, and 8.5% did not know. Of 594 who answered the zoster question, 78% gave a negative response, 4% did not know, and 104 (17%) answered yes. Of these, 83 reported 1 episode; 12 had 2; 5 had 3; and 1 each reported 5, 6, and 15 episodes. Of 51 patients tested for VZV IgG (44 "yes," 4 "no," and 3 "I don't know" answers to the question of whether they had chickenpox), 48 were seropositive; the 3 seronegative all had reported having had chickenpox. The high rate of MS patients reporting prior chickenpox infection is comparable with previous reports. A substantial proportion of MS patients, estimated to be higher than an age-matched general population, report single or multiple episodes of zoster. These data are useful for consideration of immunosuppressive treatments and/or VZV and zoster vaccination.
Cerebral metabolism in newborn dogs during reversible asphyxia (1977)

Abstract Acute, systemic asphyxia was induced in paralyzed, lightly anesthetized 1‐ to 2‐day‐old dogs by respiratory arrest. Measurements of arterial blood pressure, acid‐base balance, and concentrations of pyruvate, lactate, and glucose in blood were correlated with electroencephalographic activity and with concentrations of high‐energy phosphates and glycolytic substrates in the cerebral cortex. Most animals tolerated 15 minutes, but not 20 minutes, of asphyxia with apparently normal behavioral recovery. Asphyxia was always accompanied by bradycardia, systemic hypotension, and a progressive decline in arterial pH; the concentration of blood lactate and the lactate/pyruvate ratio rose. Blood glucose levels were unaffected, at least during the first 10 minutes. Concentrations of glucose and phosphocreatine in cerebral cortex declined rapidly during asphyxia to low levels, but levels of adenosine triphosphate remained within normal limits for up to 5 minutes despite the fact that electrical activity in brain ceased within 2 minutes. The intravenous injection of carbon black into animals asphyxiated for 2 1/2 , 5, 10, or 15 minutes revealed substantial reductions in blood flow to brain during asphyxia; however, relative to the cerebral cortex, brainstem structures received a preferential blood supply.
Cyclo-oxygenase 2 expression is associated with angiogenesis and lymph node metastasis in human breast cancer (2002)

These data suggest that COX-2 expression is associated with angiogenesis, lymph node metastasis, and apoptosis in human breast cancer. Moreover, these results warrant further studies with larger series of patients to confirm the association with short disease free survival in patients with breast cancer.
Early growth and drought avoidance in Douglas-fir seedlings (1972)

Although the Douglas-fir region of the northwestern United States and British Columbia is frequently considered to have a common silviculture, obstacles to successful regeneration of the species range from competing vegetation and mammals on the mesic sites to extreme drought and heat on the xeric southerly exposures in northern California and southern Oregon. Recent reports on Douglas-fir, Pseudotsuga menziesii (Mirb.) Franco, by Ferrell and Woodard (1), Pharis and Ferrell (8), and Zavitkovski and Ferrell (11, 12) describe studies that demonstrated differences by seed source of response to drought stress of Douglas-fir seedlings under laboratory and greenhouse conditions. Our study was undertaken to determine whether planting stock raised from seed collected from mesic and xeric sites would demonstrate a differential survival under drought stress. And, further, to determine whether such survival differences were correlated with definite seedling parameters.
Aging Affects Two Modes of Nanoleakage Expression in Bonded Dentin (2003)

Water sorption into resin-dentin interfaces precedes hydrolytic degradation. We hypothesized that these processes are morphologically manifested by the uptake of ammoniacal silver nitrate, which is thought to trace hydrophilic domains and water-filled channels within matrices. Water sorption is thought to be nonuniform and can be traced by the use of silver nitrate. Human teeth bonded with an experimental filled-adhesive were aged in artificial saliva (experimental) or non-aqueous mineral oil (control). Specimens retrieved for up to a 12-month period were immersed in 50 wt% ammoniacal silver nitrate and examined by transmission electron microscopy for identification of the changes in their silver uptake. Reticular silver deposits initially identified within the bulk of hybrid layers in the experimental group were gradually reduced over time, but were subsequently replaced by similar deposits that were located along the hybrid layer-adhesive interface. Silver uptake in water-binding domains of the adhesive layers increased with aging, resulting in water tree formation. These water-filled channels may act as potential sites for hydrolytic degradation of resin-dentin bonds.
Laboratory Evaluation of Fire-Resistant Turbine Fluids (1957)

The trend toward higher steam temperatures has increased the possibility of fires from oil leaks in turbine systems. This has stimulated investigation of fire-resistant fluids for this application. Various chemical-base fluids are currently being studied in the laboratory, and at least one type is undergoing field trial. There is a need for development of suitable test methods for evaluating these fluids. Also, the use of such fluids will involve new design and operational considerations. The ASTM may be able to contribute to the resolution of these matters.
Performance Evaluation of Scheduling in IEEE 802.16 Based Wireless Mesh Networks (2006)

We propose an efficient centralized scheduling algorithm in IEEE 802.16 based wireless mesh networks (WMN) to provide high qualified wireless multimedia services. Our algorithm takes special attention on the relay function of the mesh nodes in a transmission tree which is seldom studied in previous research. Some important design metrics, such as fairness, channel utilization and transmission delay are considered in this scheduling algorithm. IEEE 802.16 employs TDMA and the selection policy for scheduled links in a time slot will definitely impact the system performance. We evaluated the proposed algorithm with four selection criteria through extensive simulations and the results are instrumental for improving the performance of IEEE 802.16 based WMNs in terms of link scheduling
<title>Implementation of an embedded image-based tracker in a distributed architecture</title> (2002)

A major purpose of targeting systems is to ease operator workload by keeping the line of sight (LOS) pointed on a designated target. Our airborne electro-optic targeting system uses the latest technology to accomplish this end. If the latitude, longitude and altitude (geo-position) of a stationary ground target are known in advance, this system can use the aircraft's GPS-aided inertial navigation system to keep the LOS pointed at the target. If the operator wants to point at a target whose geo-position is unknown, this system can compute latitude, longitude and altitude using LOS attitude and range. The role of the image-based tracker in this system is to track airborne or moving ground targets and to compensate for drift and other limitations of geo-point for stationary ground targets. The algorithms implemented in our targeting system are not only processing intensive, but also diverse, incorporating a variety of information from numerous sources in order to provide the operator with the best LOS control solution for a given scenario. This, balanced against the low latency requirements of a real-time control system, has resulted in a software design that spans four separate processor boards. We present the implementation of a successful real-time embedded image-based tracker on a distributed architecture.
ChemInform Abstract: SYNTHESIS AND CHARACTERIZATION OF ISOMERIC CIS‐ AND TRANS‐PYRRONE MODEL COMPOUNDS (1973)

Abstract Pyrromellitsäuredianhydrid (I) und o‐Phenylendiamin (II) liefert über die Zwischenstufen (III) die beiden isomeren Bis‐benzimidazol‐benzoldicarbonsäuren (IV) und (VII), aus denen die Cyclisierungsprodukte (V) bzw. (VIII) sowie durch Spaltung mit Methylat in reversibler Reaktion die Ester (VI) bzw. (IX) zugänglich sind.
Identification of Cocaine and Its Metabolites in Human Urine in the Presence of Ethyl Alcohol (1979)

A complete methodology was developed using extraction procedures for identification of cocaine, its major metabolites, common diluents, and other abused drugs from human urine case studies. Techniques utilized included screening by thin-layer chromatography, subsequent on-column alkylation for identification, and chemical ionization mass spectroscopy using methane as the reagent gas for confirmation. Data regarding the detection of cocaethylene, a cocaine homologue, was verified in the presence of ethyl alcohol as a biotransformation product with ethylecgonine.
Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants. (1995)

Cell plate formation in tobacco root tips and synchronized dividing suspension cultured tobacco BY-2 cells was examined using cryofixation and immunocytochemical methods. Due to the much improved preservation of the cells, many new structural intermediates have been resolved, which has led to a new model of cell plate formation in higher plants. Our electron micrographs demonstrate that cell plate formation consists of the following stages: (1) the arrival of Golgi-derived vesicles in the equatorial plane, (2) the formation of thin (20 +/- 6 nm) tubes that grow out of individual vesicles and fuse with others giving rise to a continuous, interwoven, tubulo-vesicular network, (3) the consolidation of the tubulo-vesicular network into an interwoven smooth tubular network rich in callose and then into a fenestrated plate-like structure, (4) the formation of hundreds of finger-like projections at the margins of the cell plate that fuse with the parent cell membrane, and (5) cell plate maturation that includes closing of the plate fenestrae and cellulose synthesis. Although this is a temporal chain of events, a developing cell plate may be simultaneously involved in all of these stages because cell plate formation starts in the cell center and then progresses centrifugally towards the cell periphery. The "leading edge" of the expanding cell plate is associated with the phragmoplast microtubule domain that becomes concentrically displaced during this process. Thus, cell plate formation can be summarized into two phases: first the formation of a membrane network in association with the phragmoplast microtubule domain; second, cell wall assembly within this network after displacement of the microtubules. The phragmoplast microtubules end in a filamentous matrix that encompasses the delicate tubulo-vesicular networks but not the tubular networks and fenestrated plates. Clathrin-coated buds/vesicles and multivesicular bodies are also typical features of the network stages of cell plate formation, suggesting that excess membrane material may be recycled in a selective manner. Immunolabeling data indicate that callose is the predominant lumenal component of forming cell plates and that it forms a coat-like structure on the membrane surface. We postulate that callose both helps to mechanically stabilize the early delicate membrane networks of forming cell plates, and to create a spreading force that widens the tubules and converts them into plate-like structures. Cellulose is first detected in the late smooth tubular network stage and its appearance seems to coincide with the flattening and stiffening of the cell plate.
Simple and Efficient Transgenesis with Meganuclease Constructs in Zebrafish (2009)

In the past, microinjection of plasmid DNA into early embryos represented the state of the art to generate transgenic zebrafish. However, this approach suffers significant drawbacks (mosaic distribution of the injected transgene, late transgene integration at high copy numbers, low transgenesis frequency), making the generation of transgenic lines a laborious task. Coinjection of I-SceI meganuclease with a reporter construct flanked by I-SceI sites overcomes these problems by earlier transgene integration into the host genome. Here, we provide an optimized protocol for I-SceI meganuclease-mediated transgenesis in zebrafish. This simple protocol provides a reliable method to transiently test tissue-specific reporter expression of meganuclease constructs in injected embryos (F0). Furthermore, it substantially facilitates the generation of multiple stable transgenic lines increasing transgenesis frequencies up to 45%, compared with 5% without I-SceI. The reliable reporter activity in F0 and the improved transgenesis frequency make this protocol a powerful tool for use in gain- and loss-of-function, cell tracing, and cell labeling experiments.