gebhart/patrepeval huggingface dataset:
Development of New Strategies Using Extracellular Vesicles Loaded with Exogenous Nucleic Acid (2020)

Gene therapy is a therapeutic strategy of delivering foreign genetic material (encoding for an important protein) into a patient's target cell to replace a defective gene. Nucleic acids are embedded within the adeno-associated virus (AAVs) vectors; however, preexisting immunity to AAVs remains a significant concern that impairs their clinical application. Extracellular vesicles (EVs) hold great potential for therapeutic applications as vectors of nucleic acids due to their endogenous intercellular communication functions through their cargo delivery, including lipids and proteins. So far, small RNAs (siRNA and micro (mi)RNA) have been mainly loaded into EVs to treat several diseases, but the potential use of EVs to load and deliver exogenous plasmid DNA has not been thoroughly described. This review provides a comprehensive overview of the principal methodologies currently employed to load foreign genetic material into EVs, highlighting the need to find the most effective strategies for their successful clinical translations.
GaN HEMT: Dominant Force in High-Frequency Solid-State Power Amplifiers (2015)

The gallium nitride (GaN) high-electron-mobility transistor (HEMT) has emerged as the dominant force in high-frequency solid-state power amplifiers (PAs)-not that it does not have competition. Silicon (Si) bipolar junction transistors (BJTs) and Si laterally diffused metal-oxide-semiconductor (LDMOS) field-effect transistors (FETs) are still commercially available. They are viable alternatives to GaN HEMTs in aerospace/defense applications such as L-band transponders/interrogators for the identification friend or foe; Link 16 data links; electronic warfare; and surveillance radar; and, in the case of Si LDMOSs, commercial cellular base stations. These older technologies can be favored due to their mature heritage, good performance, and low cost. The pseudomorphic HEMT (PHEMT) is ubiquitous in microwave and millimeter-wave power applications. Vacuum electron devices (VEDs) still reign in the regime of brute power. The GaN HEMT has been displacing these technologies as it has matured and costs have come down.
Smac Mimetics Activate the E3 Ligase Activity of cIAP1 Protein by Promoting RING Domain Dimerization (2011)

The inhibitor of apoptosis (IAP) proteins are important ubiquitin E3 ligases that regulate cell survival and oncogenesis. The cIAP1 and cIAP2 paralogs bear three N-terminal baculoviral IAP repeat (BIR) domains and a C-terminal E3 ligase RING domain. IAP antagonist compounds, also known as Smac mimetics, bind the BIR domains of IAPs and trigger rapid RING-dependent autoubiquitylation, but the mechanism is unknown. We show that RING dimerization is essential for the E3 ligase activity of cIAP1 and cIAP2 because monomeric RING mutants could not interact with the ubiquitin-charged E2 enzyme and were resistant to Smac mimetic-induced autoubiquitylation. Unexpectedly, the BIR domains inhibited cIAP1 RING dimerization, and cIAP1 existed predominantly as an inactive monomer. However, addition of either mono- or bivalent Smac mimetics relieved this inhibition, thereby allowing dimer formation and promoting E3 ligase activation. In contrast, the cIAP2 dimer was more stable, had higher intrinsic E3 ligase activity, and was not highly activated by Smac mimetics. These results explain how Smac mimetics promote rapid destruction of cIAP1 and suggest mechanisms for activating cIAP1 in other pathways.
Hereditary Spastic Paraplegia: Genetic Heterogeneity and Genotype-Phenotype Correlation (1999)

Hereditary spastic paraplegia (HSP) is a group of disorders whose primary symptom is insidiously progressive, lower extremity spasticity and weakness. Neuropathological analysis of "pure" HSP reveals axonal degeneration that is maximal in the terminal portions of the longest descending and ascending tracts (crossed and uncrossed corticospinal tracts to the legs and fasciculus gracilis, respectively). HSP may be transmitted as an X-linked, autosomal recessive, or autosomal dominant trait, each of which is genetically heterogeneous: mutations in different genes cause clinically similar disorders. To date, there are at least three genetic loci for X-linked HSP; at least three genetic loci for autosomal recessive HSP; and at least six genetic loci for autosomal dominant HSP. The genetic basis for three of these twelve forms of HSP have been discovered. One form of autosomal recessive HSP (on chromosome 16) is due to mutations in the paraplegin gene, which encodes a mitochondrial protein homologous to metalloproteases. One form of X-linked HSP is caused by mutations in the proteolipoprotein gene, an intrinsic myelin protein. Mutation in this gene also causes the dysmyelinating disorder, Pelizeaus-Merzbacher disease. X-linked spastic paraplegia can be caused also by mutations in the L1CAM gene. This review summarizes our current understanding of genetic heterogeneity and genotype-phenotype correlation in HSP.
A topological Area Coverage Algorithm for Indoor Vacuuming Robot (2007)

A novel complete area coverage algorithm is presented for autonomous vacuuming robots working in completely unknown indoor environments. The algorithm works by segments the environment into smaller regions. Each smaller region is covered in zigzag pattern. A topological world map based on natural landmarks, such as wall corners and edges, is built incrementally while the robot systematically cleans the environments. The natural landmarks are added as nodes in a partial map as they are discovered. A three-layer BP network classification is designed to identify the natural landmarks. A novel feature of the map is the use of topology to store coverage information. An environment is completely covered only when the world map is fully defined, that is, fully connected. The simulation and real robot experiments prove that the proposed algorithm is valid.
Neuromorphic Event-Based Slip Detection and Suppression in Robotic Grasping and Manipulation (2020)

Slip detection is essential for robots to make robust grasping and fine manipulation. In this paper, a novel dynamic vision-based finger system for slip detection and suppression is proposed. We also present a baseline and feature based approach to detect object slips under illumination and vibration uncertainty. A threshold method is devised to autonomously sample noise and object feature events in real-time to improve slip detection and suppression. Moreover, a fuzzy based suppression strategy using incipient slip feedback is proposed for regulating the grip force. A comprehensive experimental study of our proposed approaches under uncertainty and system for high-performance precision manipulation are presented. We also propose a slip metric to evaluate such performance quantitatively. For a class of objects, results indicate that the system can effectively detect incipient slip events at a sampling rate of 2kHz (Δt = 500μs) and suppress them before a gross slip occurs. The event-based approach holds promises to high precision manipulation task requirement in industrial manufacturing and household services.
Predicting battery life with early cyclic data by machine learning (2019)

Abstract This work applies machine learning tools to achieve the early prediction of commercial battery life. We compared the prediction accuracy of different machine learning algorithms to the battery database. Among various algorithms, the decision tree (DT) method exhibits the highest accuracy of 95.2% to predict whether the battery can maintain above 80% initial capacity after 550 cycles. Using the initial two cycles of data, DT proposes that the change of discharge capacity is the main feature to estimate the lifetime type of batteries. Given the first 100 cycles, the factor with the maximum weight turns to the internal resistance for estimating the battery lifetime.
Development and application of millimeter wave radar sensors for underground mining (2005)

This paper defines the issues that are required for the development of a successful underground range measurement sensor. It considers various options, including laser and sonar implementations, before focusing on a millimeter-wave frequency modulated continuous wave radar. The implementations of radar sensors for simple ranging and three-dimensional cavity profiling are then discussed before some data obtained in underground mines is presented to verify the radar performance through thick dust and vapor.
Twin-wire SAW welding of 70 kg class high strength steel (2013)

The twin-wire SAW welding process was investigated using Baosteel 70 kg class high strength steel,and the properties of the joints were studied using conventional single and double-faced grooves as well as an optimized groove.The results showed that by using an optimized double-faced groove,a small root face,and no back gouging,a small welding heat input was achieved and thus the joint strength and toughness were improved significantly.Also,removing back gouging reduced the labor required in the process.The weld reinforcement and deformation were observed to be rather small.
Effects of including a ruminally protected lipid supplement in the diet on the fatty acid composition of beef muscle (2003)

Enhancing the polyunsaturated fatty acid (PUFA) and decreasing the saturated fatty acid content of beef is an important target in terms of improving the nutritional value of this food for the consumer. The present study examined the effects of feeding a ruminally protected lipid supplement (PLS) rich in PUFA on the fatty acid composition of longissimus thoracis muscle and associated subcutaneous adipose tissue. Animals were fed ad libitum on grass silage plus one of three concentrate treatments in which the lipid source was either Megalac (rich in palmitic acid; 16 : 0) or PLS (soybean, linseed and sunflower-seed oils resulting in an 18 : 2n-6:18 : 3n-3 value of 2.4:1). Treatment 1 contained 100 g Megalac/kg (Mega, control); treatment 2 (PLS1) contained 54 g Megalac/kg with 500 g PLS/d fed separately; treatment 3 (PLS2) contained no Megalac and 1000 g PLS/d fed separately. The PLS was considered as part of the overall concentrate allocation per d in maintaining an overall forage:concentrate value of 60:40 on a DM basis. Total dietary fat was formulated to be 0.07 of DM of which 0.04 was the test oil. Total intramuscular fatty acids (mg/100 g muscle) were decreased by 0.31 when feeding PLS2 compared with Mega (P<0.05). In neutral lipid, the PLS increased the proportion of 18 : 2n-6 and 18 : 3n-3 by 2.7 and 4.1 on diets PLS1 and PLS2 v. Mega, respectively. Similar responses were noted for these fatty acids in phospholipid. The amounts or proportions of 20 : 4n-6, 20 : 5n-3 or 22 : 6n-3 were not influenced by diet whereas the amounts and proportions of 22 : 4n-6 and 22 : 5n-3 in phospholipid were decreased with inclusion of the PLS. The amounts of the saturated fatty acids, 14 : 0, 16 : 0 and 18 : 0, in neutral lipid were on average 0.37 lower on treatment PLS2 compared with Mega. Feeding the PLS also decreased the proportion of 16 : 0 in neutral lipid. The amount of 18 : 1n-9 (P=0.1) and the amount and proportion of 18 : 1 trans (P<0.01) were lower on treatments PLS1 and PLS2 in neutral lipid and phospholipid. Conjugated linoleic acid (cis-9, trans-11) was not influenced by diet in the major storage fraction for this fatty acid, neutral lipid. The PUFA:saturated fatty acids value was increased markedly (x2.5) with inclusion of the PLS (P<0.001) while the Sigman-6 : n-3 value increased slightly (x1.2; P=0.015). The results suggest that the protected lipid used, which was rich in PUFA, had a high degree of protection from the hydrogenating action of rumen micro-organisms. The PLS resulted in meat with a lower content of total fat, decreased saturated fatty acids and much higher 18 : 2n-6 and 18 : 3n-3. The net result was a large shift in polyunsaturated: saturated fatty acids, 0.28 v. 0.08, on feeding PLS2 compared with Mega, respectively.
Reservoir heterogeneity and fracture parameter determination using electrical image logs and petrophysical data (a case study, carbonate Asmari Formation, Zagros Basin, SW Iran) (2019)

Abstract Assessment of reservoir and fracture parameters is necessary to optimize oil production, especially in heterogeneous reservoirs. Core and image logs are regarded as two of the best methods for this aim. However, due to core limitations, using image log is considered as the best method. This study aims to use electrical image logs in the carbonate Asmari Formation reservoir in Zagros Basin, SW Iran, in order to evaluate natural fractures, porosity system, permeability profile and heterogeneity index and accordingly compare the results with core and well data. The results indicated that the electrical image logs are reliable for evaluating fracture and reservoir parameters, when there is no core available for a well. Based on the results from formation micro-imager (FMI) and electrical micro-imager (EMI), Asmari was recognized as a completely fractured reservoir in studied field and the reservoir parameters are mainly controlled by fractures. Furthermore, core and image logs indicated that the secondary porosity varies from 0% to 10%. The permeability indicator indicates that zones 3 and 5 have higher permeability index. Image log permeability index shows a very reasonable permeability profile after scaling against core and modular dynamics tester mobility, mud loss and production index which vary between 1 and 1000 md. In addition, no relationship was observed between core porosity and permeability, while the permeability relied heavily on fracture aperture. Therefore, fracture aperture was considered as the most important parameter for the determination of permeability. Sudden changes were also observed at zones 1-1 and 5 in the permeability trend, due to the high fracture aperture. It can be concluded that the electrical image logs (FMI and EMI) are usable for evaluating both reservoir and fracture parameters in wells with no core data in the Zagros Basin, SW Iran.
AC impedance testing for valve regulated cells (2003)

The author describes how the AC impedance of a valve regulated lead acid (VRLA) cell can be an indicator of the condition of the standby battery system. Used in conjunction with load cycle testing impedance testing identifies cells requiring replacement, reduces performance testing, and reinforces confidence in the battery system. Test results are presented which confirm the impedance test as the first true diagnostic test for VRLA cells. The condition of VRLA battery systems is examined, common failure modes are identified, test regimens are described, and actual test data are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Neurochemical dementia diagnostics in Alzheimer’s disease: where are we now and where are we going? (2011)

Neurochemical dementia diagnostics (NDD) is a routine laboratory tool used in the diagnostic process for patients with neurodegenerative disorders, such as Alzheimer's disease. Currently, two groups of biomarkers analyzed in the cerebrospinal fluid are considered - namely amyloid-β peptides and Tau proteins - along with the hyperphosphorylated forms of the latter (pTau). Current directions in the development of NDD include the following: search for novel biomarkers with improved analytical or diagnostic performance; optimization of the analysis of the biomarkers already available (e.g., by improved quality control and interlaboratory comparison of results); applications of novel technologies enabling better management of patient samples; and search for biomarkers in the blood. This article presents the state-of-the-art in the field of cerebrospinal fluid-based NDD, and also summarizes some of the hypotheses of how the future development of NDD tools might look.
Amplitude apodizers encoded onto Fresnel lenses implemented on a phase-only spatial light modulator (2001)

We show that both a lens and a nonuniform amplitude transmission filter can be encoded simultaneously onto a twisted nematic liquid-crystal spatial light modulator (SLM) working in the phase-only mode. The inherent equivalent apodization that is due to the pixelated structure of the SLM is compensated for. In addition, different types of nonuniform transmission pupil such as transverse apodizing, transverse hyperresolving, and axial hyperresolving (multifocusing) filters are implemented. The excellent agreement between numerical and experimental results shows the capability of this method to encode amplitude apodizers on a phase-only SLM.
Compounds That Contract on Heating (1998)

Various mechanisms to explain negative thermal expansion in oxides are presented. Our search for compounds having this unusual property has focused on the mechanism based on transverse thermal motion of oxygen in M−O−M linkages. Such a mechanism can give strong negative thermal expansion over broad temperature ranges. Examples of compounds where this mechanism operates to give negative thermal expansion are ZrW2O8, the Sc2W3O12 family, NbOPO4, AlPO4-17, and faujasite SiO2.
Carbonation and related reactions of poly(styryl)lithium (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCarbonation and related reactions of poly(styryl)lithiumRoderic P. Quirk, Jian Yin, and Lewis J. FettersCite this: Macromolecules 1989, 22, 1, 85–90Publication Date (Print):January 1, 1989Publication History Published online1 May 2002Published inissue 1 January 1989https://pubs.acs.org/doi/10.1021/ma00191a017https://doi.org/10.1021/ma00191a017research-articleACS PublicationsRequest reuse permissionsArticle Views376Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Highly selective reactive ion etch process for InP-based device fabrication using methane/hydrogen/argon (1993)

The etch rates of GaInAs and AlInAs were characterized using a mixture of methane, hydrogen, and argon as a function of self-bias voltage. Effectively infinite etch selectivity between GaInAs and AlInAs was found for voltages below 200 V. This highly selective etch process was applied to the gate recess of a high electron mobility transistor device, and preliminary device measurements were made.
Anion binding by multidentate Lewis acids: a DFT study (2001)

Density functional studies of model tribora-macrocyclic Lewis acids indicate extremely strong binding of the methyl anion, which in some cases exceeds that of B(C6F5)3. In addition anion selectivity for the fluoride ion is implied by binding energies ca. 200 kJ mol−1 greater than those of other halides.
The Jackson Laboratory repository: model selection for immunology research (82.10) (2007)

Abstract The mouse continues as one of the most important model organisms. The Jackson Laboratory Repository serves as a centralized facility for developing, rederiving, cryopreserving and distributing mouse models. The Repository contains a large repertoire of mouse strains related to immunology research that have applications in studies of basic immunology, innate immunity, cancer research, autoimmunity, allergic diseases, and infectious diseases. Selecting appropriate mutant mice is often difficult. For example, mice devoid of CD4+ T cells are found in 3 different strains including a Cd4 −/− strain (B6.129S2-Cd4tm1Mak/J), a strain where MHC class II genes were deleted (B6.129-H2dlAb1-Ea/J), or a targeted mutation in the class II transactivator (B6.129S2-C2tatm1Ccum/J). A wide variety of cre-expressing strains, targeted mutants with loxP-flanked genes and tetracycline regulated transactivator-expressing mice are available that require careful selection. Other factors for model selection include mutation characteristics related to mechanism of action, cell types affected, effects of the mutation on breeding and lifespan, and other associated phenotypes. The strain background including breeding performance, MHC haplotype, disease resistance/susceptibilility and known mutations, such as hemolytic complement deficiency found in a subset of inbred strains, could confound results. Supported by NCRR (RR09781, RR11083, RR16049), NIA, HHMI, and donations from several private foundations
Microvesicles containing miRNAs promote muscle cell death in cancer cachexia via<i>TLR7</i> (2014)

MicroRNAs (miRNAs) are small, noncoding RNAs that regulate gene expression and, in cancers, are often packaged within secreted microvesicles. The cachexia syndrome is a debilitating state of cancer that predominantly results from the loss of skeletal muscle mass, which is in part associated with apoptosis. How tumors promote apoptosis in distally located skeletal muscles has not been explored. Using both tumor cell lines and patient samples, we show that tumor-derived microvesicles induce apoptosis of skeletal muscle cells. This proapoptotic activity is mediated by a microRNA cargo, miR-21, which signals through the Toll-like 7 receptor (TLR7) on murine myoblasts to promote cell death. Furthermore, tumor microvesicles and miR-21 require c-Jun N-terminal kinase activity to regulate this apoptotic response. Together, these results describe a unique pathway by which tumor cells promote muscle loss, which might provide a great insight into elucidating the causes and treatment options of cancer cachexia.
Fluorometric Sucrose Evaluation for Sugar Beet (2004)

Sucrose is the economic product from sugar beet. Disease resistance is often available in low-sucrose genotypes and, prior to the deployment of such novel genes as available into the cultivated spectrum, selection for increased sucrose content is required during introgression. The objective of this work was to evaluate a relatively rapid and inexpensive enzymatic-fluorometric microtiter plate assay for sucrose quantification in sugar beet root dry matter, both for progeny testing in the greenhouse and for evaluation of field-grown mother roots. As determined using HPLC, sucrose content in diverse populations of sugar and table beet assayed over various developmental stages ranged from 0.213 to 2.416 mmol g(-1) of dry matter, and these values were used as references for both refractometry and enzymatic-fluorometric assay. As expected, refractometric analysis generally overestimated sucrose content. Enzymatic-fluorometric analyses were reasonably well correlated with HPLC results for young greenhouse-grown root tissues (R2 = 0.976), and less so with older field-grown roots (R2 = 0.605), for unknown reasons. Enzymatic-fluorometric assays may be best deployed for progeny testing of young seedlings.