gebhart/patrepeval huggingface dataset:
A glow discharge anemometer (1934)

A glow discharge at atmospheric pressure is responsive to air velocity, and may be used as an anemometer. The properties of such a glow are discussed in this paper, and data relating to glow current, voltage, glow length, and air velocity are presented. It is also shown that the glow discharge responds to rapid fluctuations in air velocity and is therefore a practical device for investigating turbulence. A comparison of such an anemometer with the previously used hot wire method is given.
Nucleotide sequence of avian carcinoma virus MH2: two potential onc genes, one related to avian virus MC29 and the other related to murine sarcoma virus 3611. (1984)

The 5.2-kilobase (kb) RNA genome of avian carcinoma virus MH2 has the genetic structure 5'-delta gag (0.2 kb)- mht (1.2 kb)-myc (1.4 kb)-c (0.4 kb)-poly(A) (0.2 kb)-3'. delta gag is a partial retroviral core protein gene, mht and myc are cell-derived MH2-specific sequences, and c is the 3'-terminal retroviral vector sequence. Here we have determined the nucleotide sequence of 3.5 kb from the 3' end of delta gag to the 3' end of molecularly cloned proviral MH2 DNA, in order to elucidate the genetic structure of the virus and to compare it with other mht - and myc-containing oncogenic viruses as well as with the chicken proto-myc gene. The following results were obtained: (i) delta gag- mht forms a hybrid gene with a contiguous reading frame of 2682 nucleotides that terminates with a stop codon near the 3' end of mht . The 3' 969 nucleotides of mht up to the stop codon are 80% sequence related to the onc-specific raf sequence of murine sarcoma virus 3611 (94% homologous at the deduced amino acid level). (ii) The myc sequence is preceded by an RNA splice acceptor site shared with the cellular proto-myc gene, beyond which it is colinear up to a 3'-termination codon and 40 noncoding nucleotides with the myc sequences of avian retrovirus MC29 and chicken proto-myc. Thus, myc forms, together with a 5' retroviral exon, a second MH2-specific gene. (iii) myc is followed by the 3'-terminal c region of about 400 nucleotides, which is colinear with that of Rous sarcoma virus except for a substitution near the 5' end of the long terminal repeat. It is concluded that MH2 contains two genes with oncogenic potential, the delta gag- mht gene, which is closely related to the delta gag-raf transforming gene of MSV 3611, and the myc gene, which is related to the transforming gene of MC29. Furthermore, it may be concluded that the cellular proto-onc genes, which on sequence transduction become viral onc genes, are a small group because among the 19 known onc sequences, 5 are shared by different taxonomic groups of viruses of which the mht /raf homology is the closest determined so far.
Superpipelined adder designs (2002)

The superpipelining concept is applied to adder designs. Superpipelined designs of ripple carry, carry select, conditional sum, and carry lookahead adders are presented as examples. It is shown that the carry skip adder cannot be superpipelined. Results for the total number of latches required, the number of pipelining stages, and the total gate counts are presented. It is shown that the superpipelined carry lookahead adder is faster and less complex than the other adders.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Insights into the CtrA regulon in development of stress resistance in obligatory intracellular pathogen <i>Ehrlichia chaffeensis</i> (2011)

Ehrlichia chaffeensis is an obligate intracellular bacterium that causes human monocytic ehrlichiosis. Ehrlichiae have a biphasic developmental cycle consisting of dense-cored cells (DCs) and reticulate cells (RCs). Isolated DCs are more stress resistant and infectious than RCs. Here, we report that a response regulator, CtrA was upregulated in human monocytes at the late growth stage when DCs develop. E. chaffeensis CtrA bound to the promoters of late-stage transcribed genes: ctrA, ompA (peptidoglycan-associated lipoprotein), bolA (stress-induced morphogen) and surE (stationary-phase survival protein), which contain CtrA-binding motifs, and transactivated ompA, surE and bolA promoter-lacZ fusions in Escherichia coli. OmpA was predominantly expressed in DCs. E. chaffeensis binding to and subsequent infection of monocytes were inhibited by anti-OmpA IgG. E. chaffeensis BolA bound to the promoters of genes encoding outer surface proteins TRP120 and ECH_1038, which were expressed in DCs, and transactivated trp120 and ECH_1038 promoter-lacZ fusions. E. chaffeensis bolA complemented a stress-sensitive E. coli bolA mutant. E. coli expressing E. chaffeensis SurE exhibited increased resistance to osmotic stress. Our results suggest that E. chaffeensis CtrA plays a role in co-ordinating development of the stress resistance for passage from the present to the next host cells through its regulon.
Optical-Field-Induced Birefringence and Freedericksz Transition in a Nematic Liquid Crystal (1981)

Optical-field-induced birefringence in nematic 4-cyano-4\ensuremath{'}-pentylbiphenyl was measured with cw pump and probe beams, and the optical-field-induced Freedericksz transition was observed for the first time. The results are in quantitative agreement with the theoretical prediction.
Reverse-time wave-field extrapolation, imaging, and inversion (1988)

Abstract The scattered wave field propagated backward in time into an arbitrary background medium is related via a volume integral to perturbations in velocity about the background, which are expressed as a scattering potential. In general, there is no closed-form expression for the kernel of this integral representation, although it can be expressed asymptotically as a superposition of plane waves backpropagated from the receiver array. When the receiver array completely surrounds the scatterer, the kernel reduces to the imaginary part of the Green's function for the background medium.This integral representation is used to relate the images obtained by imaging algorithms to the actual scattering potential. Two such relations are given: (1) for the migrated image, obtained by deconvolving the extrapolated field with the incident field; and (2) for the reconstructed image, obtained by applying a one-way wave operator to the extrapolated field and then deconvolving by the incident field. The migrated image high-lights rapid changes in the scattering potential (interfaces), whereas the reconstructed image can, under ideal conditions, be a perfect reconstruction of the scattering potential. 'Ideal' conditions correspond to (1) weak scattering about a smoothly varying background medium, (2) a receiver array with full angular aperture, and (3) data of infinite bandwidth.Images obtained from a multioffset vertical seismic profile (VSP) illustrate some of the practical differences between the two imaging algorithms. The reconstructed image shows a much clearer picture of the target (a reef structure), in part because the one-way imaging operator eliminates artifacts caused by the limited aperture of the receiver array.
An electrowetting-based microfluidic platform for magnetic bioassays (2010)

Here we present our recent work on a droplet-based microfluidic device for manipulating microliter-sized droplets. By replacing the formerly used common dielectric SiO2 with Si3N4 and applying a 33 nm thick Teflon top layer to create a hydrophobic surface, we successfully lowered the actuation voltage from 450 V to 50 Vdc/40 Vac. Sputtered HfO2 with high dielectric constant was also investigated as an insulator, which could reproducibly yield thin defect-free insulation layers and lower the actuation voltage to less than 40 V.
Biotechnological Applications of Protein Splicing (2019)

Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.
A review of islanding detection methods for distributed resources (2004)

Islanding detection methods for distributed resources are reviewed. Special emphasis is put on some of the detecting systems based on "active methods" which have been developed in Japan for rotating machine type distributed generations. Then, reduction of the islanding detection sensitivity with multiple distributed generations is discussed. Finally, a new concept of an islanded operation is introduced.
Synthesis of some benzofuronaphthyridines and benzofuronaphthyridine derivatives (1993)

Abstract The new benzofuro[2,3‐ b ]naphthyridine ring system was prepared. 2,4‐Dichloro‐3‐( o ‐methoxyphenyl)naphthyridines 3a and 3b were obtained by chlorination of hydroxynaphthyridinones 2a and 2b . Demethylation followed by cyclization of 3a and 3b afforded 11‐chlorobenzofuro[2,3‐ b ]naphthyridines 4a and 4b . Hydrogenolysis of these 11‐chlorobenzofuronaphthyridines gave the parent benzofuro[2,3‐ b ]naphthyridines 5a and 5b . The analog 4b was also converted to 11‐methoxybenzofuro[2,3‐ b ][1,8]naphthyridine ( 6 ).
Soft Robotic Manipulation and Locomotion with a 3D Printed Electroactive Hydrogel (2018)

Electroactive hydrogels (EAH) that exhibit large deformation in response to an electric field have received great attention as a potential actuating material for soft robots and artificial muscle. However, their application has been limited due to the use of traditional two-dimensional (2D) fabrication methods. Here we present soft robotic manipulation and locomotion with 3D printed EAH microstructures. Through 3D design and precise dimensional control enabled by a digital light processing (DLP) based micro 3D printing technique, complex 3D actuations of EAH are achieved. We demonstrate soft robotic actuations including gripping and transporting an object and a bidirectional locomotion.
Nuclear magnetic resonance studies and conformational analysis of bicyclic inhibitors of angiotensin-converting enzyme. Part 2. The octahydro-6H-pyridazo[1,2-a][1,2]diazepines (1986)

The conformations are determined of a number of bicyclic heterocycles synthesised as part of a programme related to the design of inhibitors of angiotensin-converting enzyme (ACE). In these compounds, a six-membered ring is fused to a seven-membered ring via a diaza bridge. By detailed 1H n.m.r. spectroscopy, the 6-oxo series is shown to be rigid with the axial 1-carboxy anchoring the system. In the 6-deoxy series some flexibility in the seven-membered ring is apparent, though nitrogen inversion is prevented. X-Ray diffraction, molecular graphics, and MNDO calculations are employed to assist in the conformational analyses. The three-dimensional arrangement of the three enzyme-binding groups in these compounds provides very potent inhibitors (l50 1.6–3nM).
Automatic high‐precision measurements of the location and width of Kikuchi bands in electron backscatter diffraction patterns (1998)

The demands for reliability and precision of crystal orientation data obtained through automatic analysis of electron backscattering patterns (EBSPs) in the SEM result in similar demands on the quality of the band position data which is provided by an image analysis procedure. This paper describes a new image processing procedure which is capable of providing accurate measurements of the location and width of typically 10–15 bands in digitized EBSPs of average quality. The new procedure is based on the Hough transform (HT) for line detection, and employs a special backmapping technique for generating two simplified HTs which separately focus on bright and dark lines in the images. A coordinated search for peaks in the two HTs leads to precise estimates of both the position and the width of bands in the patterns. A visual evaluation of the data produced by the new procedure shows that it performs significantly better than the conventional procedure with regard to both reliability and precision. Additionally, the measured band width data are fairly precise and can be used for obtaining a more robust and reliable indexing of the bands. Finally, the computational costs of the new procedure are smaller than for the conventional procedure.
Stereoselective Microbial Dehalorespiration with Vicinal Dichlorinated Alkanes (2003)

The suspected carcinogen 1,2-dichloroethane (1,2-DCA) is the most abundant chlorinated C(2) groundwater pollutant on earth. However, a reductive in situ detoxification technology for this compound does not exist. Although anaerobic dehalorespiring bacteria are known to catalyze several dechlorination steps in the reductive-degradation pathway of chlorinated ethenes and ethanes, no appropriate isolates that selectively and metabolically convert them into completely dechlorinated end products in defined growth media have been reported. Here we report on the isolation of Desulfitobacterium dichloroeliminans strain DCA1, a nutritionally defined anaerobic dehalorespiring bacterium that selectively converts 1,2-dichloroethane and all possible vicinal dichloropropanes and -butanes into completely dechlorinated end products. Menaquinone was identified as an essential cofactor for growth of strain DCA1 in pure culture. Strain DCA1 converts chiral chlorosubstrates, revealing the presence of a stereoselective dehalogenase that exclusively catalyzes an energy-conserving anti mechanistic dichloroelimination. Unlike any known dehalorespiring isolate, strain DCA1 does not carry out reductive hydrogenolysis reactions but rather exclusively dichloroeliminates its substrates. This unique dehalorespiratory biochemistry has shown promising application possibilities for bioremediation purposes and fine-chemical synthesis.
Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children (1999)

Bordetella pertussis shows polymorphism in two proteins, pertactin (Prn) and the pertussis toxin (PT) S1 subunit, which are important for immunity. A previous study has shown antigenic shifts in these proteins in the Dutch B. pertussis population, and it was suggested that these shifts were driven by vaccination. The recent Italian clinical trial provided the opportunity to compare the frequencies of Prn and PT S1 subunit variants in strains isolated from unvaccinated children, and from children vaccinated with two acellular and one whole-cell pertussis vaccine. Four Prn variants (Prn1, Prn2, Prn3 and Prn5) were found in the 129 strains analysed. Prn1, Prn2 and Prn3 have been described previously, whereas Prn5 is a novel variant. Prn1, Prn2, Prn3 and Prn5 were found in, respectively, 6, 41, 51 and 2% of the strains. The B. pertussis strains used to produce the vaccines administered in the clinical trial were found to produce Prn1, or a type which differed from Prn1 in one amino acid. The frequency of the Prn1 variant was found to be lowest in the strains isolated from vaccinated groups, suggesting that Prn1 strains are more affected by vaccine-induced immunity than Prn2 and Prn3 strains. Only one PT S1 type (S1A) was observed in the examined strains, which was distinct from the types produced by the vaccine strains (S1B and S1D). The S1A type also predominates in the Dutch B. pertussis population. The genetic relationship among B. pertussis strains analysed by IS1002-based DNA fingerprinting revealed that three fingerprint types predominate, representing more than 70% of the strains. Prn2 strains showed a greater variety of fingerprint types compared to Prn3, suggesting that Prn3 has emerged more recently. The results are discussed in the light of vaccine-driven evolution.
Development and analysis of an adaptive noise equalizer (1995)

The paper presents the development and analysis of a narrowband adaptive noise equalizer (ANE), which can either amplify or attenuate narrowband noise. The output of the ANE system contains residual narrowband components, the amplitudes of which can be linearly and arbitrarily controlled by adjusting the gain parameter of the equalizer, thus providing the desired noise shaping capability. The characteristics of the ANE system are analyzed and applied to active noise control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Wavefield Analysis Over Large Areas Using Distributed Higher Order Microphones (2014)

Successful recording of large spatial soundfields is a prevailing challenge in acoustic signal processing due to the enormous numbers of microphones required. This paper presents the design and analysis of an array of higher order microphones that uses 2D wavefield translation to provide a mode matching solution to the height invariant recording problem. It is shown that the use of Mth order microphones significantly reduces the number of microphone units by a factor of 1/(2M + 1) at the expense of increased complexity at each microphone unit. Robustness of the proposed array is also analyzed based on the condition number of the translation matrix while discussing array configurations that result in low condition numbers. The white-noise gain (WNG) of the array is then derived to verify that improved WNG can be achieved when the translation matrix is well conditioned. Furthermore, the array's performance is studied for interior soundfield recording as well as exterior soundfield recording using appropriate simulation examples.
Need a Catalyst? Design an Enzyme (1984)

En changeant un ou plusieurs acides amines sur les enzymes on s'apercoit qu'il est possible d'etudier leur fonction catalytique et peut-etre meme de produire de nouveaux catalyseurs
Measurement of telomere length in haematopoietic cells using <i>in situ</i> hybridization techniques (2000)

The DNA of human chromosomes terminates in several kilobases of telomere repeats that are gradually lost with; age and with replication in vitro. Defective telomere maintenance has been shown to be causally linked to cell cycle exit and apoptosis. In order to overcome the limitations imposed by Southern blotting, we have established a quantitative fluorescence in situ hybridization (Q-FISH) technique. This technique allows estimation of telomere length in specific chromosome arms from metaphase cell preparations. Furthermore, we have extended quantitative in situ hybridization to flow cytometry (flow FISH) in order to obtain information on the mean telomere repeat content in suspended cells. Telomere length in granulocytes, monocytes, CD8 and CD4 T lymphocytes and natural killer cells was found to differ slightly in the peripheral blood of adults. However, strikingly longer telomeres were observed in B lymphocytes (approximately 1.3 kb longer), suggesting a functional role for telomere maintenance in this cell subset. In summary, Q-FISH and flow FISH represent new methods for measuring telomere length in single cells and allow studies of telomere dynamics in haematopoietic subpopulations at various stages of normal and abnormal antigen responses.
Alloantibodies in von Willebrand disease (2013)

The development of alloantibodies against von Willebrand factor (VWF) represents a rare but serious complication of treatment of von Willebrand disease (VWD), occurring in ~5% to 10% of type 3 VWD patients. Affected patients can present with a range of symptoms, including lack or loss of hemostatic response to infused VWF concentrates up to anaphylactic reactions in rare cases. It is classically reported in multitransfused patients and occurs most frequently in patients with partial or complete VWF gene deletions. A positive family history of anti-VWF antibodies also appears to be a risk factor. There is a lack of standardization of laboratory methods for antibody identification and characterization. Issues of variability in laboratory approaches as well as the rarity of the complication act as a barrier to future studies. Recombinant factor VIII as well as bypassing agents and immune tolerance have been reported as effective treatments; however, aside from case reports, little exists in the literature to guide management. The imminent clinical availability of recombinant VWF has prompted a resurgence of interest in this area. Additional study is warranted to address the deficiencies in our understanding of this treatment complication.
Effective-index matrix analysis of distributed feedback semiconductor lasers (1992)

A method is developed for calculating the threshold gain in distributed feedback (DFB) semiconductor lasers consisting of a four-layer waveguide. Each corrugation region is expressed by a transfer matrix comprising the complex effective index, and the threshold calculation is reduced to the matrix multiplication. It is found that a periodic variation of the effective gain and loss induced by the modulation of the optical confinement factors gives rise to the threshold gain difference between the two adjacent modes on both sides of the Bragg frequency in standard DFB lasers without facet reflections. The absorption loss in the guiding layer increases the amplitude of the gain modulation, resulting in the increase of the threshold gain difference.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Thermal properties and flame retardancy of an ether-type UV-cured polyurethane coating (2010)

A new UV-reactive monomer piperazine-N,N-bis(acryloxyethylaryl-phosphoramidate) (N-PBAAP) containing phosphorus and nitrogen was synthesized and used as flame retardant for an ether-type UV-cured polyurethane acrylate (PUA) coating. The thermal properties of the PUA films were investigated by thermogravimetric analysis (TGA) in air and nitrogen atmosphere. The TGA results showed that the incorporation of N-PBAAP can obviously enhance the char residue of the PUA coatings. From the TGA and real time Fourier transformed infrared spectroscopy (RT-FTIR) results, different degradation behaviors were observed in the PUA coatings with different N-PBAAP content. The combustibility of the PUA coatings was evaluated by microscale combustion calorimeter (MCC). The MCC results revealed that the addition of N-PBAAP in the coatings can significantly reduce the peak Heat Release Rate (pHRR), Heat Release Capacity (HRC) and the Total Heat Release (THR) of the samples. Furthermore, dynamical mechanical thermal analysis (DMA) was employed to examine the viscoelastic properties of the PUA films. It was found that the incorporation of N-PBAAP in the formulation can bring in more functional groups to the coatings, which results in an increase of the glass transition temperature (Tg) and cross linking density (XLD) of the films.