gebhart/patrepeval huggingface dataset:
Stable Chloroplast Transformation of the Unicellular Red Alga<i>Porphyridium</i> Species (2002)

Abstract Red algae are extremely attractive for biotechnology because they synthesize accessory photosynthetic pigments (phycobilins and carotenoids), unsaturated fatty acids, and unique cell wall sulfated polysaccharides. We report a high-efficiency chloroplast transformation system for the unicellular red microalgaPorphyridium sp. This is the first genetic transformation system for Rhodophytes and is based on use of a mutant form of the gene encoding acetohydroxyacid synthase [AHAS(W492S)] as a dominant selectable marker. AHAS is the target enzyme of the herbicide sulfometuron methyl, which effectively inhibits growth of bacteria, fungi, plants, and algae. Biolistic transformation of synchronized Porphyridium sp. cells with the mutant AHAS(W492S) gene that confers herbicide resistance gave a high frequency of sulfomethuron methyl-resistant colonies. The mutant AHAS gene integrated into the chloroplast genome by homologous recombination. This system paves the way for expression of foreign genes in red algae and has important biotechnological implications.
Modification of transport properties of ion‐exchange membranes VIII. Changes in properties of anion exchange membranes on introduction of hydrophobic groups (1989)

Abstract A copolymer membrane composed of 4‐vinylpyridine‐styrene‐divinylbenezene was reacted with long chain alkyl bromides (octyl bromide, dodecyl bromide, and hexadecyl bromide) in order to introduce hydrophobic groups into the anion exchange membrane by the quaternization reaction. The resulting membranes show a marked increased in fixed ion concentration as a result of lowered water content. Although the electrical resistance of the membranes increased slightly, the apparent diffusion coefficient of hydrochloric acid through the membranes decreased markedly. Complete quaternization of the membranes by methyl iodide after reaction with the long‐chain alkyl bromides caused an increase in the water content and in the apparent diffusion coefficient of the acid and a decrease in the current efficiency in electrodialysis of the acid. This is explained by the high water content of the anion exchange membranes quaternized by methyl iodide.
Ultra-Thin Silicon based Piezoelectric Capacitive Tactile Sensor (2016)

This paper presents an ultra-thin bendable silicon based tactile sensor, in a piezoelectric capacitor configuration, realized by wet anisotropic etching as post-processing steps. The device is fabricated over bulk silicon, which is thinned down to 35 μm from an original thickness of 636 μm. Dicing of thin membrane is achieved by low cost novel technique of Dicing before Etching. The piezoelectric capacitor is composed of polyvinylidene fluoride trifluoroethylene (PVDF-TrFE), which present an attractive avenue for tactile sensing as they respond to dynamic contact events (which is critical for robotic tasks), easy to fabricate at low cost and are inherently flexible. The sensor exhibits enhanced piezoelectric properties, thanks to the optimization of the poling procedure. The sensor capacitive behaviour is confirmed using impedance analysis and the electro-mechanical characterization is done using TIRA shaker setup.
Intracellular accumulation of aggregated pyroglutamate amyloid beta: convergence of aging and Aβ pathology at the lysosome (2012)

Deposition of aggregated amyloid beta (Aβ) is a major hallmark of Alzheimer's disease (AD)-a common age-related neurodegenerative disorder. Typically, Aβ is generated as a peptide of varying lengths. However, a major fraction of Aβ peptides in the brains of AD patients has undergone posttranslational modifications, which often radically change the properties of the peptides. Aβ3(pE)-42 is an N-truncated, pyroglutamate-modified variant that is abundantly present in AD brain and was suggested to play a role early in the pathogenesis. Here we show that intracellular accumulation of oligomeric aggregates of Aβ3(pE)-42 results in loss of lysosomal integrity. Using a novel antibody specific for aggregates of AβpE3, we show that in postmortem human brain tissue, aggregated AβpE3 is predominantly found in the lysosomes of both neurons and glial cells. Our data further demonstrate that AβpE3 is relatively resistant to lysosomal degradation, which may explain its accumulation in the lysosomes. The intracellular AβpE3 aggregates increase in an age-dependent manner. The results presented in this study support a model where Aβ pathology and aging converge, leading to accumulation of the degradation-resistant pE-modified Aβ in the lysosomes, lysosomal dysfunction, and neurodegeneration.
Fractional Reyes‐Style Adaptive Tessellation for Continuous Level of Detail (2014)

Abstract In this paper we present a fractional parametric splitting scheme for Reyes‐style adaptive tessellation. Our parallel algorithm generates crack‐free tessellation from a parametric surface, which is also free of sudden temporal changes under animation. Continuous level of detail is not addressed by existing Reyes‐style methods, since these aim to produce subpixel‐sized micropolygons, where topology changes are no longer noticeable. Using our method, rendering pipelines that use larger triangles, thus sensitive to geometric popping, may also benefit from the quality of the split‐dice tessellation stages of Reyes. We demonstrate results on a real‐time GPU implementation, going beyond the limited quality and resolution of the hardware tessellation unit. In contrast to previous split‐dice methods, our split stage is compatible with the fractional hardware tessellation scheme that has been designed for continuous level of detail.
A model of the mechanical design process based on empirical data (1988)

This paper describes the task/episode accumulation model (TEA model) of non-routine mechanical design, which was developed after detailed analysis of the audio and video protocols of five mechanical designers. The model is able to explain the behavior of designers at a much finer level of detail than previous models. The key features of the model are (a) the design is constructed by incrementally refining and patching an initial conceptual design, (b) design alternatives are not considered outside the boundaries of design episodes (which are short stretches of problem solving aimed at specific goals), (c) the design process is controlled locally, primarily at the level of individual episodes. Among the implications of the model are the following: (a) CAD tools should be extended to represent the state of the design at more abstract levels, (b) CAD tools should help the designer manage constraints, and (c) CAD tools should be designed to give cognitive support to the designer.
The Effect of Innate Immunity on Autoimmune Diabetes and the Expression of Toll-Like Receptors on Pancreatic Islets (2004)

Viral infections have previously been implicated as a trigger of autoimmune diabetes. In this study, we compared a viral mimic with other microbial components derived from bacteria in triggering diabetes development in C57BL/6-rat insulin promoter-B7.1 mice that do not normally develop diabetes. It is striking that only the viral mimic induced the development of diabetes in our model system. Further mechanistic studies suggest that diabetes is induced, in part, by the combination of direct recognition of this virus-like stimulus by pancreatic islets through the expression of the innate immune receptor, Toll-like receptor 3. In addition, the functions of APCs are up-regulated, and this could stimulate islet Ag-reactive T cells that will attack beta cells leading to autoimmune diabetes.
Damage mechanisms and process optimization for photomasks with sub-resolution assist features (2008)

Sub-Resolution Assist Features (SRAFs) are typically the smallest features on a photomask and amplify many of the challenges in mask manufacturing. During the initial stages of process development, resist feature adhesion is the dominate damage mechanism. Throughout mask fabrication, the influence of inherent material properties and wet processing can eventually delaminate SRAFs. The various mechanisms that cause SRAF damage will be presented systematically. Process optimization steps to address the failure mechanisms will be presented. Data illustrating the improved process window for small features will be included.
La reforma del mercado laboral de 2012 se ajusta a la Constitución: análisis de la sentencia del Tribunal Constitucional 119/2014, de 16 de julio (BOE, 15 de agosto) (2014)

La STC 119/2014 resuelve los temas de constitucionalidad mas espinosos y complejos de la reforma laboral de 2012, plasmada en la L 3/2012. Declara que los preceptos impugnados se ajustan a los mandatos constitucionales. Tras exponer las razones de decidir de la sentencia analizada, el estudio se adentra en la metodologia aplicada por la mayoria de magistrados del TC (9) y por los magistrados que han suscrito un voto particular (3), asi como en la valoracion de las cuestiones de dogmatica-juridico-laboral implicadas. Tambien se recuerda el ATC 43/2014 de caracter precedente.
Sphingosine-1-Phosphate Transporters as Targets for Cancer Therapy (2014)

Sphingosine-1-phosphate (S1P) is a pleiotropic lipid mediator that regulates cell survival, migration, the recruitment of immune cells, angiogenesis, and lymphangiogenesis, all of which are involved in cancer progression. S1P is generated inside cancer cells by sphingosine kinases then exported outside of the cell into the tumor microenvironment where it binds to any of five G protein coupled receptors and proceeds to regulate a variety of functions. We have recently reported on the mechanisms underlying the "inside-out" signaling of S1P, its export through the plasma membrane, and its interaction with cell surface receptors. Membrane lipids, including S1P, do not spontaneously exchange through lipid bilayers since the polar head groups do not readily go through the hydrophobic interior of the plasma membrane. Instead, specific transporter proteins exist on the membrane to exchange these lipids. This review summarizes what is known regarding S1P transport through the cell membrane via ATP-binding cassette transporters and the spinster 2 transporter and discusses the roles for these transporters in cancer and in the tumor microenvironment. Based on our research and the emerging understanding of the role of S1P signaling in cancer and in the tumor microenvironment, S1P transporters and S1P signaling hold promise as new therapeutic targets for cancer drug development.
Enhanced Cell Adhesion to the Dimpled Surfaces of Golf-Ball-Shaped Microparticles (2014)

Engineering surface morphology as in honeycomb-structured planar films is of great importance for providing new potential application and improved performance in biomedical fields. We demonstrate potential new applications for the uniform biocompatible golf-ball-shaped microparticles that resembles 3D feature of honeycomb-structured film. Dimple size controllable golf-ball-shaped microparticles were fabricated by microfluidic device. Surface dimples not only can act as picoliter beaker but also enhance cell adhesion without any chemical modification of the surface.
Generation of a pH gradient in an immobilized enzyme system (1993)

Several examples of two-step sequential reactions exist where, because of the poor equilibrium conversion by the first reaction, it is desirable to conduct the two reactions simultaneously. In such a scheme, the product of the first reaction is continuously removed by the second reaction, thus not allowing the first reaction to approach chemical equilibrium. Therefore, the first reaction is allowed to proceed in the desired direction at an appreciable rate. However, in many biochemical applications where enzyme catalysts are involved, the enzyme's activities are strong functions of pH. Where the pH optima of the first and second reaction differ by three to four units, the above reaction scheme would be difficult to implement. In these cases, the two reactions can be separated by a thin permeable membrane across which the desired pH gradient is maintained. In this article, it was shown, both by theory and experiment, that a thin, flat membrane of immobilized urease can accomplish this goal when one face of the membrane is exposed to the acidic bulk solution (pH(b) = 4.5) containing a small quantity of urea (0.01 M). In this particular case, the ammonia that was produced in the membrane consumed the incoming hydrogen ions and thus maintained the desired pH gradient. Experimental results indicate that with sufficient urease loading, the face of the membrane opposite to the bulk solution could be maintained at a pH that would allow many enzymes to realize their maximum activities ( approximately 7.5). It was also found that this pH gradient could be maintained even in the presence of a buffer, which greatly enhances the transport of protons into the membrane.
Chemical Dynamics in Solution (1990)

The majority of reactions carried out by chemists take place in liquid solutions. Only within the last 10-15 years, however, has it become possible to study such reactions in molecular detail.
Thermal Degradation Chemistry of Alkyl Quaternary Ammonium Montmorillonite (2001)

The thermal stability of organically modified layered silicate (OLS) plays a key role in the synthesis and processing of polymer-layered silicate (PLS) nanocomposites. The nonoxidative thermal degradation of montmorillonite and alkyl quaternary ammonium-modified montmorillonite were examined using conventional and high-resolution TGA combined with Fourier transform infrared spectroscopy and mass spectrometry (TG−FTIR−MS) and pyrolysis/GC−MS. The onset temperature of decomposition of these OLSs was approximately 155 °C via TGA and 180 °C via TGA−MS, where TGA−MS enables the differentiation of water desorbtion from true organic decomposition. Analysis of products (GC−MS) indicates that the initial degradation of the surfactant in the OLS follows a Hoffmann elimination reaction and that the architecture (trimethyl or dimethyl), chain length, surfactant mixture, exchanged ratio, or preconditioning (washing) does not alter the initial onset temperatures. Catalytic sites on the aluminosilicate layer reduce thermal stability of a fraction of the surfactants by an average of 15−25 °C relative to the parent alkyl quaternary ammonium salt. Finally, the release of organic compounds from the OLS is staged and is associated with retardation of product transfer arising from the morphology of the OLS. These observations have implications to understanding the factors impacting the interfacial strength between polymer and silicate and the subsequent impact on mechanical properties as well as clarifying the role (advantageous or detrimental) of the decomposition products in the fundamental thermodynamic and kinetic aspects of polymer melt intercalation.
Phytoalexin Accumulation in <i>Arabidopsis thaliana</i> during the Hypersensitive Reaction to <i>Pseudomonas syringae</i> pv <i>syringae</i> (1992)

Inoculation of leaves of Arabidopsis thaliana (L.) Heynh. with the wheat pathogen, Pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation. No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, Xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (pH 6.9). Phytoalexin accumulation was correlated with the restricted in vivo growth of P. s. syringae. A phytoalexin was purified by a combination of reverse phase flash chromatography, thin layer chromatography, followed by reverse phase high performance liquid chromatography. The Arabidopsis phytoalexin was identified as 3-thiazol-2'-yl-indole on the basis of ultraviolet, infrared, mass spectral, (1)H-nuclear magnetic resonance, and (13)C-nuclear magnetic resonance data.
The changing concept of soil quality (1995)

Soil quality concerns are not new, but each time we come to them we use a slightly different approach because our understanding of soils has increased and the questions that scientists and society ask have changed. Productivity and suitability for different beneficial uses of soil have been major themes in soil quality, but there are other approaches. It is useful to consider the changing concept of soil quality so we can put our present concerns in the context of the other ideas as we seek to reverse soil degradation and improve soil quality

Suitability for various beneficial uses is probably the oldest and one of the most frequently used concepts of soil quality. Often this concept is related to the quantity of crops produced, but it can also be related to quality. Soil health, as it determines human health, is expressed through crop quality. Because soil is a unique habitat for a wide diversity of biota, various biological parameters have been used in defining soil quality. A further refinement is to define soil quality in terms of the various functions that soils perform in ecosystems, such as recycling of nutrients, partitioning …
Surface Preparation and Corrosion Behavior of Titanium Alloys for Surgical Implants (1983)

Surface preparation and corrosion behavior of titanium alloys were studied; both topics deal with the formation of surface oxide films. When metals are prepared for surgical implant use, an effort is made to produce an optimum surface. Effects of the surface treatments on surface morphology, surface film composition, and structure are shown in transmission electron photomicrographs and electron diffraction patterns. Roughened surfaces were produced, and surface films of titanium oxide (TiO) or titanium dioxide (TiO2) or both occurred on some specimens. Sodium titanate (NaO · xTiO2) occurred on the specimens washed in sodium hydroxide (NaOH) solution. Open circuit potential versus time curves have been measured to show some electrochemical effects of various surface treatments. The results show that titanium alloys immersed in Hanks' physiological solution reach the same final open-circuit potential after approximately two weeks' exposure regardless of prior surface treatment. Measurements of the anodic polarization behavior of titanium alloys and other surgical implant alloys show the effects of alloy composition and the testing solution on the passive region and breakdown potentials of these materials. In general, for titanium alloys, the differences are not great, but the presence of nickel results in a significant lowering of the breakdown potential. The materials studied were titanium, Ti-6Al-4V, titanium-nickel (memory alloy), Ti-13Cu-4Ni, and Ti-4.5Al-5Mo-1.5Cr. In all cases titanium materials are more corrosion resistant in Hanks' solution than cobalt-chromium-molybdenum Type 316L stainless steel, or cobalt-nickel-chromium alloys. Repassivation measurements show the rapid formation and the high degree of stability of the protective film on titanium and titanium alloys.
The Applicability of Semiconducting Layered Materials for Electrochemical Solar Energy Conversion (1980)

Abstract Semiconducting transition metal dichalcogenides with layer structure belong to the most stable electrode materials in electrochemical solar cells. The photoelectrochemical reactions however, are complicated by the surface anisotropy. Results of photoelectrochemical cells with n‐type MoSe 2 and WSe 2 demonstrate the importance of surface morphology for the energy conversion efficiency. — The optimal energy conversion yield of these layered semiconductors has turned out being lower than theoretically expected from their band gaps. It is shown that this is connected with the existence of an indirect band gap which restricts light absorption in a considerable energy quantum range above the band gap. Besides this, surface defects and inhomogeneities reduce the quantum yield by increased surface recombination. — Photoelectrochemical hydrogen evolution has been investigated with p‐type WSe 2 . It is shown that a considerable energy gain can be reached by the catalytic action of platinum deposits on the surface of the electrode. This is explained in terms of surface state catalysis.
Biochemical Evaluation of Patients with Cancer-Associated Hypercalcemia (1980)

In 50 consecutive patients with cancer-associated hypercalcemia, we measured nephrogenous cyclic AMP, tubular phosphorus threshold, fasting calcium excretion, plasma 1,25-dihydroxyvitamin D, and immunoreactive parathyroid hormone as determined by four region-specific antiserums. Nephrogenous cyclic AMP excretion was elevated in 41 patients and suppressed in nine (means, 5.85 vs. 0.51 nmol per 100 ml of glomerular filtrate). There was no overlap between these groups. When compared with 15 patients with primary hyperparathyroidism, the group with increased cyclic AMP excretion had similar reductions in tubular phosphorus threshold; higher fasting calcium excretion (means, 0.66 vs. 0.25 mg per 100 ml of glomerular filtrate, P < 0.01); marked reductions in 1,25-dihydroxyvitamin D (means, 20 vs. 83 pg per milliliter, P < 0.001); and lower levels of immunoreactive parathyroid hormone in all four assays. The data suggest that elevated excretion of nephrogenous cyclic AMP may be a useful marker of humorally mediated cancer-associated hypercalcemia, that this type of hypercalcemia is common, that the humoral factor responsible for this syndrome is not native 1-84 parathyroid hormone, and that the various subtypes of cancer-associated hypercalcemia are biochemically distinguishable from primary hyperparathyroidism.
Hollow glass waveguides for broadband infrared transmission (1994)

Broadband hollow glass waveguides have been fabricated with losses as low as 0.15 dB/m at 10.6 microm. We make these hollow glass waveguides by coating the inside of polyimide-coated silica-glass tubing with a metallic layer followed by a thin dielectric coating of a metal halide. The bore sizes of the guides range from 320 to 700 microm, and we have made lengths as long as 3 m. The bending radii of the waveguides are less than 5 cm for bore sizes less than 500 microm. We have used these waveguides to deliver greater than 80 W of CO(2) laser power and 5 W of Er:YAG laser power. The hollow glass guides are inexpensive, robust, and quite flexible and therefore a good infrared fiber for power and sensor applications.
Versatile Gas-Liquid Partition Chromatography Apparatus (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVersatile Gas-Liquid Partition Chromatography ApparatusG. K. Ashbury, A. J. Davies, and J. W. DrinkwaterCite this: Anal. Chem. 1957, 29, 6, 918–925Publication Date (Print):June 1, 1957Publication History Published online1 May 2002Published inissue 1 June 1957https://doi.org/10.1021/ac60126a017Request reuse permissionsArticle Views33Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-Alertsclose Get e-Alerts