gebhart/patrepeval huggingface dataset:
A multisubunit factor, CstF, is required for polyadenylation of mammalian pre-mRNAs. (1990)

We have purified and characterized a factor required for accurate polyadenylation of mammalian pre-mRNAs in vitro. This factor, called cleavage-stimulation factor (CstF), is composed of three distinct polypeptide subunits of 77, 64, and 50 kD. Using monoclonal antibodies directed against the 64- and 50-kD subunits, we show that CstF is required for efficient cleavage of polyadenylation substrates. Furthermore, CstF present in unfractionated nuclear extracts interacts with pre-mRNAs containing the signal sequence AAUAAA, but not AAGAAA, in such a manner that the 64-kD subunit can be cross-linked to the RNA by UV light. This polypeptide is thus identical to the previously described 64-kD nuclear protein that binds to AAUAAA-containing RNAs. Finally, indirect immunofluorescence of fixed cells indicates that CstF is distributed diffusely throughout the nucleus in a granular pattern distinct from the "speckled" pattern displayed by factors involved in pre-mRNA splicing, but similar to that of heterogeneous nuclear ribonucleoproteins. A model is presented in which CstF binds specifically to nascent RNA polymerase II transcripts and, by interacting with other factors, results in a rapid initiation of 3'-end processing of pre-mRNAs.
Multiple Defects of Natural Killer Cells in Cancer Patients: Anarchy, Dysregulated Systemic Immunity, and Immunosuppression in Metastatic Cancer (2020)

We have previously demonstrated that natural killer (NK) cells are the main immune effectors that can mediate selection and differentiation of different cancer stem cells and undifferentiated tumors via lysis and secreted or membrane-bound interferon-γ and tumor necrosis factor-α, respectively. This leads to growth inhibition and tumor metastasis curtailment. In this review, we present an overview of our findings on NK cell biology and its significance in selection and differentiation of stem-like tumors using in vitro and in vivo studies conducted in nonobese diabetic/severe combined immunodeficiency (scid)/interleukin-Rγ--, humanized-bone-marrow/liver/thymus (hu-BLT) mice, and those of human cancer patients. Moreover, we present recent advances in NK cell expansion and therapeutic delivery and discuss the superiority of allogeneic supercharged NK cells over their autologous counterparts for cancer treatment. We review potential loss of NK cell numbers and function at neoplastic and preneoplastic stages of tumorigenesis as a potential mechanism for pancreatic cancer induction and progression. We believe that NK cells should be placed highly in the armamentarium of tumor immunotherapy due to their indispensable role in targeting cancer stem-like/poorly differentiated tumors and a variety of other key NK cell functions that are discussed in this report, including their role in CD8+ T-cell expansion and targeting gene knockout or dedifferentiated tumors. The combination of allogeneic supercharged NK cells and other immunotherapeutic strategies such as oncolytic viruses, antibody-dependent cellular cytotoxicity-inducing antibodies, checkpoint inhibitors, chimeric antigen receptor (CAR)-T cells and CAR-NK cells, chemotherapeutics, and radiotherapeutic strategies can be used for optimal eradication of tumors.
Laparoscopic Lumbar Discectomy: Case Report (1991)

Laparoscopic lumbar discectomy is an extension of two accepted technologies, laparoscopy and percutaneous discectomy. A young male with an L5-S1 disc herniation had the anterior aspect of the lumbosacral disc exposed laparoscopically. The disc space was entered with a trephine under fluoroscopic and video control. The discectomy was carried out with curettes and rongeurs. Evoked potentials monitored continuously, reverted to normal with decompression of the S1 nerve root. This was accomplished in an outpatient setting with minimal use of oral narcotics.
CuO Nanoparticles from the Strongly Hydrated Ionic Liquid Precursor (ILP) Tetrabutylammonium Hydroxide: Evaluation of the Ethanol Sensing Activity (2012)

The sensing potential of CuO nanoparticles synthesized via precipitation from a water/ionic liquid precursor (ILP) mixture was investigated. The particles have a moderate surface area of 66 m(2)/g after synthesis, which decreases upon thermal treatment to below 5 m(2)/g. Transmission electron microscopy confirms crystal growth upon annealing, likely due to sintering effects. The as-synthesized particles can be used for ethanol sensing. The respective sensors show fast response and recovery times of below 10 s and responses greater than 2.3 at 100 ppm of ethanol at 200 °C, which is higher than any CuO-based ethanol sensor described so far.
In Silico Identification of Potent COVID-19 Main Protease Inhibitors from FDA Approved Antiviral Compounds and Active Phytochemicals through Molecular Docking: A Drug Repurposing Approach (2020)

The Novel Coronavirus (COVID-19) is a positive-sense single-stranded RNA ((+)ssRNA) virus. The COVID-19 Main Proteases play very important role in the propagation of the Novel Coronavirus (COVID-19). It has already killed more than 8000 people around the world and thousands of people are getting infected every day. Therefore, it is very important to identify a potential inhibitor against COVID-19 Main Proteases to inhibit the propagation of the Novel Coronavirus (COVID-19). We have applied a drug repurposing approach of computational methodology, depending on the synergy of molecular docking and virtual screening techniques, aimed to identify possible potent inhibitors against Novel Coronavirus (COVID-19) from FDA approved antiviral compounds and from the library of active phytochemicals. On the basis of recently resolved COVID-19 Main Protease crystal structure (PDB:6LU7), the library of 100 FDA approved antiviral compounds and 1000 active components of Indian Medicinal Plants extracted for screening against COVID-19 Main Protease. The compounds were further screened using Pyrex virtual screening tool and then best inhibitors, top 19 compounds optimally docked to the COVID-19 Main Protease structure to understand the participation of specific amino acids with inhibitors at active sites. Total 19 best compounds were identified after screening based on their highest binding affinity with respect to the other screened compounds. Out of 19, 6 best compounds were further screened based on their binding affinity and best ADME properties. Nelfinavir exhibited highest binding energy -8.4 kcal/mol and strong stability with the TRP207, ILE281, LEU282, PHE3, PHE291, GLN127, ARG4, GLY283, GLU288, LYS5, LYS137, TYR126, GLY138, TYR126, SER139 and VAL135 amino acid residues of COVID-19 Main Protease participating in the interaction at the binding pocket. In addition to Nelfinavir (-8.4), Rhein (-8.1), Withanolide D (-7.8), Withaferin A (-7.7), Enoxacin (-7.4), and Aloe-emodin (-7.4) also showed good binding affinity and best ADME properties. Our findings suggest that these compounds can be used as potential inhibitors against COVID-19 Main Protease, which could be helpful in inhibiting the propagation of the Novel Coronavirus (COVID-19). Moreover, further in vitro and in vivo validation of these findings would be very helpful to bring these inhibitors to next level study.
Enhancing data locality by using terminal propagation (1996)

Terminal propagation is a method developed in the circuit placement community for adding constraints to graph partitioning problems. This paper adapts and expands this idea, and applies it to the problem of partitioning data structures among the processors of a parallel computer. We show how the constraints in terminal propagation can be used to encourage partitions in which messages are communicated only between architecturally near processors. We then show how these constraints can be handled in two important partitioning algorithms, spectral bisection and multilevel-KL. We compare the quality of partitions generated by these algorithms to each other and to partitions generated by more familiar techniques.
Electro-Generation of Furfural Alcohol in Ni-P-MCMC/mCS Bipolar Membrane Equipped Electrolysis Cell (2011)

Based on the actual conditions during industrial production, this paper proposes an improvement method and solution to the typical grinding process model and derives a mathematical model of grinding process that’s suitable for industrial production. Sampling analysis and laboratory experiments of the production process were conducted, the derived model was tested, and the results show that particle size distribution curve obtained from the improved model is similar to the actual product, which means that the improved model can be applied to simulate industrial grinding process.
ARYLSULFUR TRIFLUORIDES AND PENTAFLUORIDES (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTARYLSULFUR TRIFLUORIDES AND PENTAFLUORIDESWilliam A. SheppardCite this: J. Am. Chem. Soc. 1960, 82, 17, 4751–4752Publication Date (Print):September 1, 1960Publication History Published online1 May 2002Published inissue 1 September 1960https://doi.org/10.1021/ja01502a083Request reuse permissionsArticle Views737Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (260 KB) Get e-Alertsclose Get e-Alerts
Two-Dimensional MEMS Stage Integrated With Microlens Arrays for Laser Beam Steering (2010)

A novel microelectromechanical stage with one uniaxial set of combs capable of 2-D actuation is presented. A polymer microlens array (MLA) is mounted vertically onto the stage. Driven at resonance, the stage deflects 124 <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\mu\hbox{m}$</tex></formula> out of plane and 34 <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex Notation="TeX">$\mu\hbox{m}$</tex></formula> in plane. Finally, laser beam steering is demonstrated using two cascaded MLAs. <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\hfill$</tex></formula> [2010-0201]
δ Opioid Receptor Agonism Preserves the Retinal Pigmented Epithelial Cell Tight Junctions and Ameliorates the Retinopathy in Experimental Diabetes (2019)

This set of experiments strongly indicates that the DOR agonism preserves RPE tight junctions and reduces the early markers of retinopathy in model of diabetes. These novel findings designate DOR as a potential therapeutic tool to treat DR with preservation of the RPE tight junction proteins.
Serum Retinol Levels and the Risk of Fracture (2003)

Our findings, which are consistent with the results of studies in animals, as well as in vitro and epidemiologic dietary studies, suggest that current levels of vitamin A supplementation and food fortification in many Western countries may need to be reassessed.
Ferroelectricity in<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:mi mathvariant="normal">Pb</mml:mi><mml:mrow><mml:mo>(</mml:mo><mml:msub><mml:mi mathvariant="normal">Zr</mml:mi><mml:mn>0.5</mml:mn></mml:msub><mml:msub><mml:mi mathvariant="normal">Ti</mml:mi><mml:mn>0.5</mml:mn></mml:msub><mml:mo>)</mml:mo></mml:mrow><mml:msub><mml:mi mathvariant="normal">O</mml:mi><mml:mn>3</mml:mn></mml:msub></mml:mrow></mml:math>thin films: Critical thickness and 180° stripe domains (2004)

The ferroelectric properties of disorder $\mathrm{Pb}({\mathrm{Zr}}_{0.5}{\mathrm{Ti}}_{0.5}){\mathrm{O}}_{3}$ thin films are investigated with Monte Carlo simulations on the basis of a first-principles-derived Hamiltonian. It is found that there exists a critical thickness of about three unit cells $(\ensuremath{\sim}12\phantom{\rule{0.3em}{0ex}}\mathrm{\AA{}})$ below which the ferroelectricity disappears under the condition that the in-plane polarizations are suppressed by sufficient clamping effect. Above the critical thickness, periodic 180\ifmmode^\circ\else\textdegree\fi{} stripe domains with out-of-plane polarizations are formed in the systems in order to minimize the energy of the depolarizing field. The stripe period increases with increasing film thickness. The microscopic mechanism responsible for these phenomena is discussed.
Wingbeat Shape Modulation for Flapping-Wing Micro-Air-Vehicle Control During Hover (2010)

A new method of controlling a flapping-wing micro air vehicle by varying the velocity profiles of the wing strokes is presented in this manuscript. An exhaustive theoretical analysis along with simulation results show that this new method, called split-cycle constant-period frequency modulation, is capable of providing independent control over vertical and horizontal body forces as well as rolling and yawing moments using only two physical actuators, whose oscillatory motion is defined by four parameters. An actuated bob-weight is introduced to enable independent control of pitching moment. A general method for deriving sensitivities of cycle-averaged forces and moments to changes in wingbeat kinematic parameters is provided, followed by an analytical treatment for a case where the angle of attack of each wing is passively regulated and the motion of the wing spar in the stroke plane is driven by a split-cycle waveform. These sensitivities are used in the formulation of a cycle-averaged control law that successfully stabilizes and controls two different simulation models of the aircraft. One simulation model is driven by instantaneous aerodynamic forces derived from blade-element theory, while the other is driven by an empirical representation of an unsteady aerodynamic model that was derived from experiments.
How to augment the second image? Recovery of the translation scale in image to image registration (2008)

In this paper, we present an automatic pose estimation (6 DoF) technique to augment images using keyframes pre-registered to a CAD model. State of the art techniques recover the essential matrix (5 DoF) in an automatic manner, but include a manual step to align the image with the CAD reference system because the essential matrix does not provide the scale of the translation. We propose using planar structures to recover this scale automatically and to offer immediate augmentation. These techniques have been implemented in our augmented reality software. Qualitative tests are performed in an industrial environment.
Occurrence of Piscirickettsiosis-Like Syndrome in Tilapia in the Continental United States (2005)

From 2001 to 2003, tilapia (Oreochromis sp.) farms in Florida, California, and South Carolina experienced epizootics of a systemic disease causing mortality. The fish exhibited lethargy, occasional exophthalmia, and skin petechia. The gills were often necrotic, with a patchy white and red appearance. Grossly, the spleen and kidneys were granular with whitish irregular nodules throughout. Granulomatous infiltrates were observed in kidney, spleen, testes, and ovary tissues, but not in the liver. The granulomas contained pleomorphic coccoid bacteria, measuring 0.57 +/- 0.1 x 0.8 +/- 0.2 microm, that were Giemsa-positive, acid-fast-negative, and Gram-negative. The bacteria had a double cell wall, variable electron-dense and -lucent areas, and were present in the cytoplasm and within phagolysosomes. The syndrome was associated with cold stress and poor water conditions. These findings are consistent with an infectious process caused by a Piscirickettsia-like bacterium described previously in tilapia in Taiwan and Hawaii. This report involves the first identified cases of a piscirickettsiosis-like syndrome affecting tilapia in the continental United States.
Trace Element Supplementation of Cheese Whey for the Production of Feed Yeast (1983)

The stimulating effect of some heavy metals, when added to cheese whey, on growth of the yeast Candida intermedia was investigated. With copper, manganese, zinc, iron, and molybdemum addition to whey, biomass productivity was increased enormously. The process was implemented by an Austrian dairy. In a 14-m 3 jet fermentor 30,000 liters of whey were processed daily. The mash leaving the fermentor was concentrated to 12% yeast dry matter by a vacuum evaporator, and after supplementation with other nutrients was fed directly to pigs. Based on European prices, the yeast product was about 20% cheaper than soy meal.
Stochastic Automata Modeling of Certain Problems of Collective Behavior (1980)

The behavior of a collective of interacting stochastic automata in a stationary random environment is considered. Each automaton is a goal-seeking element which manipulates its strategy only as a function of the environmental response. The goals of the automata as well as the nature of their interactions are assumed to satisfy certain qualitative properties which ensure the existence and uniqueness of a Nash strategy. It is shown that, without need of any a priori information, the collective behavior of the automata converges in probability to the Nash strategy. Two applications are presented. The first concerns the process of market price formation in a competitive economy. The second concerns the optimal allocation of a unidimensional resource in the process of system operation.
Mechanical property evaluation and failure analysis of cantilevered LIGA nickel microposts (2001)

An experimental apparatus has been built to measure the elastic modulus and bending strength (modulus of rupture) of LIGA nickel posts. The apparatus uses the static cantilever beam bending approach to measure mechanical properties in a direction parallel to the growth direction. Experimental results are presented for two sets of largely identical posts constructed using an overplating method. One set was electroplated using a Watts bath, and the other set was electroplated using a sulfamate bath. For the Watts bath, the measured modulus of elasticity was slightly lower than that of bulk nickel (182 GPa), while, for the sulfamate bath, it was approximately half (93 GPa). The strength properties of the two sets of posts also differ dramatically. Microhardness measurements, Focused Ion Beam (FIB) images of grain structure, and scanning electron microscopy (SEM) micrographs of failure regions are used to further characterize and explain the differences in the results. This integrated testing approach yields a consistent set of data regarding material properties, grain size/structure and failure mechanisms. Potential sources of experimental error are also identified and improvements in experiment design are suggested to reduce these errors.
Epigallocatechin Gallate, a Constituent of Green Tea, Represses Hepatic Glucose Production (2002)

Herbs have been used for medicinal purposes, including the treatment of diabetes, for centuries. Plants containing flavonoids are used to treat diabetes in Indian medicine and the green tea flavonoid, epigallocatechin gallate (EGCG), is reported to have glucose-lowering effects in animals. We show here that the regulation of hepatic glucose production is decreased by EGCG. Furthermore, like insulin, EGCG increases tyrosine phosphorylation of the insulin receptor and insulin receptor substrate-1 (IRS-1), and it reduces phosphoenolpyruvate carboxykinase gene expression in a phosphoinositide 3-kinase-dependent manner. EGCG also mimics insulin by increasing phosphoinositide 3-kinase, mitogen-activated protein kinase, and p70(s6k) activity. EGCG differs from insulin, however, in that it affects several insulin-activated kinases with slower kinetics. Furthermore, EGCG regulates genes that encode gluconeogenic enzymes and protein-tyrosine phosphorylation by modulating the redox state of the cell. These results demonstrate that changes in the redox state may have beneficial effects for the treatment of diabetes and suggest a potential role for EGCG, or derivatives, as an antidiabetic agent.
Photoreduction of Autooxidized Albumin−Heme Hybrid in Saline Solution:  Revival of Its O<sub>2</sub>-Binding Ability (2001)

Recombinant human serum albumin (rHSA) incorporating 2-[8-[N-(2-methylimidazolyl)]octanoyloxymethyl]-5,10,15,20-tetrakis(alpha,alpha,alpha,alpha-o-pivalamido)phenylporphinatoiron(II)s (Fe(II)Ps) [rHSA-Fe(II)P] is a synthetic hemoprotein which can bind and release O(2) reversibly under physiological conditions (saline solution [NaCl]: 150 mM, pH 7.3) as do hemoglobin and myoglobin. However, the central ferrous ions of Fe(II)Ps are slowly oxidized to O(2)-inactive ferric forms. Based on the UV-vis. absorption spectroscopy, the majority of the autooxidized Fe(III)Ps in albumin are determined to be six-coordinate high-spin complexes with a proximal imidazole and a chloride anion, which show ligand-to-metal charge transfer (LMCT) absorption at 330 nm. Interestingly, photoirradiation of this LMCT band under an argon atmosphere led to reduction of the central ferric iron of Fe(III)P, allowing the revival of the O(2)-binding ability. The ratio of the photoreduction reached a maximum of 83%, which is probably due to the partial dissociation of the axial imidazole. The same photoirradiation under a CO atmosphere provides the corresponding carbonyl rHSA-Fe(II)P. Laser flash photolysis experiments revealed that the reduction was completed within 100 ns. The quantum yields (Phi) of these photoreductions were approximately 0.01.
Optical sensor for noncontact measurement of lignin content in high-speed moving paper surfaces (2005)

An optical sensor for real-time measurement of lignin content in moving paper sheets is described in this paper. The sensor is useful for high-speed automated sorting of papers according to lignin content in a mixed-waste stream for efficient recycling. The sensor measures lignin fluorescence when excited in the visible region. Results indicate that when paper surfaces are excited with a laser source (532 nm), the lignin fluorescence spectrum shows a peak near 650 nm. Measurement of this peak intensity from paper samples is a measure of lignin content and correlates linearly with the standard chemical method for lignin determination (Klasson lignin content). Newsprint samples, typically containing high lignin, produce high intensity, while ledger printing and writing grades with low lignin content produce low-fluorescence intensity. The proposed sensor configuration is discussed and results from a systematic study of variables, namely, text printed on surface, homogeneous dyed in color, printed color patches, paper thickness, sensor configuration parameters such as excitation source intensity, and distance from sample surface are reported. In dynamic tests on a simulated conveyor operating at realistic speeds, the sensor is able to reliably measure fluorescence intensities on unprinted samples without ambiguity and identify high and low lignin-containing samples.
Antagonistic effects of the endophytic fungus Meira geulakonigii on the citrus rust mite Phyllocoptruta oleivora (2007)

Findings from this study demonstrate the endophytic nature of M. geulakonigii in its interaction with grapefruits. In addition, a molecular approach was developed to specifically detect the fungus inside the grapefruit peel. This approach can be used to assess the natural occurrence of M. geulakonigii in grapefruit.