gebhart/patrepeval huggingface dataset:
Regulation of transcription and chromatin structure by pRB: Here, there and everywhere (2012)

Commitment to divide is one of the most crucial steps in the mammalian cell division cycle. It is critical for tissue and organismal homeostasis, and consequently is highly regulated. The vast majority of cancers evade proliferative control, further emphasizing the importance of the commitment step in cell cycle regulation. The Retinoblastoma (RB) tumor suppressor pathway regulates this decision-making step. Since being the subject of Knudson's 'two hit hypothesis', there has been considerable interest in understanding pRB's role in cancer. It is best known for repressing E2F dependent transcription of cell cycle genes. However, pRB's role in controlling chromatin structure is expanding and bringing it into new regulatory paradigms. In this review we discuss pRB function through protein-protein interactions, at the level of transcriptional regulation of individual promoters and in organizing higher order chromatin domains.
Modeling EAI (2002)

Software integration has been a solution to the problem of connecting dissimilar systems for many years. Integration is still a challenging issue. EAI is defined as the process of integrating enterprise systems with existing applications. Since software integration research has yielded a variety of different concepts, we survey and discuss this terminology. EAI arises as an evolution of the CASE technology approach. The main goal of this work is the modeling of EAI in order to obtain a more organized and unified view of the main aspects involved. The EAI model presented here was constructed extending the known Brown's Conceptual Model of Integration. One contribution of this paper is the identification of architectural styles and patterns and the specification of a quality model based on the ISO 9126-1 standard for enterprise systems (ES). This quality model specifies the quality characteristics that the systems in the EAI domain should hold.
In situ misemgel as a multifunctional dual-absorption platform for nasal delivery of raloxifene hydrochloride: formulation, characterization, and in vivo performance (2018)

The developed in situ misemgel matrix could be considered as a promising multifunctional platform for nasal delivery which works based on a dual-absorption mechanism.
Micelles of poly(oxyethylene)-poly(oxypropylene) block copolymer (pluronic) as a tool for low-molecular compound delivery into a cell: phosphorylation of intracellular proteins with micelle incorporated [gamma-32P]ATP. (1992)

Pluronic P85 (poly(oxyethylene)-poly(oxypropylene) block copolymer) was used for in vitro delivery of [gamma-32P]ATP into intact Jurkat cells. Negatively charged ATP molecules are not able to penetrate cell plasma membrane. Hence, exogenous [gamma-32P]ATP added to a cell culture does not participate in phosphorylation of intracellular proteins. The addition to cells of [gamma-32P]ATP solubilized in positively charged (containing dodecylamine) pluronic micelles results in considerable increase of protein phosphorylation. In this case the treatment of intact cells with alkaline phosphatase (resulting in dephosphorylation of external proteins) causes no essential decrease of [32P]-incorporation in cell proteins. That gives an evidence of delivery of solubilized ATP into a cell. Under the experimental conditions used, pluronic micelles neither influence the viability of cells nor permeabilize cell plasma membrane.
Fractionation of DNA restriction fragments with ion‐exchangers for high‐performance liquid chromatography (1986)

The fractionation abilities of several ion-exchangers of the high-performance liquid chromatography type for two sets of DNA restriction fragments, ranging from 7 base pairs (bp) to about 650 bp and differing in their mean base composition, have been studied. The ion-exchangers tested comprise the RPC-5, the 5-PW DEAE and the Mono Q as polymer-based resins, and the Nucleogens 500 and 4000, both prepared from silica beads. The results indicate that all the ion-exchangers except the 5-PW DEAE perfectly separate fragment sizes up to about 90 bp, the 5-PW DEAE separating to 45 bp only. Above 200 bp only the Mono Q resin works in a satisfactory way provided that about 100 micrograms DNA mixture, containing less than 25 fragments within the given size range, is loaded per milliliter of packed resin. Appreciable base-pair specificities were detected for most of the resins which cause substantial retardations of the d(A + T)-rich fragments with respect to the eluting salt concentration. If the latter dominate in the DNA sample, acceptable results were only obtained with the Mono Q resin when the column was operated at elevated temperature.
Neurologic Manifestations of AIDS (1987)

An understanding of the biologic characteristics and cellular tropism of human immunodeficiency virus (HIV) is critical to appreciate the diverse neurologic manifestations of HIV infection in patients with the acquired immunodeficiency syndrome (AIDS). Only carefully designed prospective studies can provide information regarding prevalence, incidence, and natural history of the full spectrum of neurologic complications of HIV infection. A degree of tropism for monocyte/macrophages and possibly for cells within the CNS seems certain. One of the most frequent complications is AIDS-related dementia, which reflects central nervous system invasion by HIV. Despite the evidence linking unchecked viral replication within the brain and progressive dementia, the basic pathogenetic mechanisms remain obscure. Further characterization of the cellular targets of HIV within the brain, and the mechanisms which ultimately lead to the dementia, is critical. The demonstration that HIV enters the central nervous system during the earliest stages of infection has major implications for antiviral agents which must penetrate brain parenchyma to clear the virus effectively. Other neurologic complications occur frequently, including myelopathies, peripheral neuropathies, opportunistic CNS infections, and CNS neoplasms. Many of these disorders are novel and incompletely characterized and their etiology is uncertain. While treatment is available for several of these conditions, it is generally not curative, and is often poorly tolerated because of adverse effects. Research directions will focus on better understanding of pathogenetic mechanisms, on earlier and more precise detection of these diverse conditions, and on improved therapeutic agents. For the future, efforts toward the development of a safe, effective vaccine are of critical importance. There are, however, already up to 2 million individuals in the United States who are already infected with HIV and who are thus at risk for developing 1 or several of these neurologic complications. Vaccination, even if it were available now, is not likely to benefit these individuals. While it is hoped that only a fraction of this infected population will develop neurologic symptoms, the prospects of an epidemic of AIDS-related dementia are ominous, particularly as antiviral therapy alone is unlikely to either eradicate the virus or restore brain function. In Africa and worldwide the numbers at risk for HIV-related diseases are enormous, and the risk factors for transmission of HIV less well defined. There, economic and medical resources are less than adequate to deal with a problem of this magnitude.
User-driven cloud transportation system for smart driving (2012)

Intelligent transportation systems (ITS) have emerged as an efficient and effective way of alleviating the traffic congestion and improving the performance of transportation systems. Key challenges of ITS in recent years include the pervasive data collection, data security, privacy preserving, large volume data processing, and intelligent analytics. These challenges lead to a revolution in ITS development by leveraging the crowdsourcing scheme and cloud computing architecture. In this paper, we propose a user-driven Cloud Transportation system (CTS) which employs a scheme of user-driven crowdsourcing to collect user data for traffic model construction and congestion prediction including data collection, filtering, modeling, intelligent computation and publish. We describe in details the application scenario, system architecture, and core CTS services model. To verify the feasibility of our approach, we have developed a prototype system which elaborated the cloud architecture and other implementation details. This paper aims to inspire further research of user-driven CTS on intelligent data processing model for smarter utilization of transportation infrastructure.
Typhus and typhuslike rickettsiae associated with opossums and their fleas in Los Angeles County, California (1992)

The recent discovery of cat fleas (Ctenocephalides felis) infected with a typhuslike rickettsia (designated the ELB agent) raises the question of whether similar rickettsial infections exist in wild cat flea populations. We verified the presence of the ELB agent and Rickettsia typhi in urban and suburban areas of Los Angeles, Calif. Opossums trapped in close proximity to the residences of human murine typhus cases in Los Angeles county and other areas within the city of Los Angeles were tested for the presence of typhus group rickettsiae by the polymerase chain reaction (PCR). The presence of rickettsiae in the spleen tissues of three opossums (n = 9) and in 66 opossum fleas (n = 205) was determined by PCR and was verified by dot blot and Southern transfer hybridization. Further analysis of the amplified PCR products generated by a series of primer pairs derived from either the 17-kDa antigen gene or the citrate synthase gene revealed that both R. typhi and the ELB agent were present in the tested samples. Dual infection was not noted in the samples; however, the fleas were infected with either R. typhi or the ELB agent. The presence of the ELB agent in the cat flea population may have implications for public health. Whether this agent is responsible for the mild cases of human murine typhus in urban and suburban areas of Los Angeles or in other endemic foci remains to be determined.
Network-aware video level encoding for uplink adaptive HTTP streaming (2015)

We study the uplink delivery of live video using adaptive HTTP streaming (AHS). In AHS, the process of simultaneously creating video levels at different rates is computationally demanding and quickly exceeds the computational capacity of mobile devices. As a remedy, we propose a network-aware video level selection approach which reduces the number of levels that need to be encoded. To this end, we develop an algorithm which selects a reduced set of video levels from a static pre-defined set based on TCP uplink throughput information. More specifically, during session start-up and after inter-RAN handovers, TCP uplink throughput information from a remote database is used while otherwise, actual TCP uplink throughput measurements are performed. We test the proposed approach in an automotive scenario to upstream the video of a vehicle's front-facing camera to a remote video portal. Our results show that our proposed network-aware video level selection approach leads to a significant reduction of the number of video levels that need to be encoded. At the same time, a similar quality of experience is achieved in terms of mean subjective quality of the delivered video segments, interrupted playback duration due to stalling events, and number of quality switches when compared to an implementation which considers the static full set of video levels.
Isozyme-specific enzyme inhibitors. 14. 5'(R)-C-[(L-homocystein-S-yl)methyl]adenosine 5'-(.beta.,.gamma.-imidotriphosphate), a potent inhibitor of rat methionine adenosyltransferases (1987)

The title compound is a covalent adduct of L-methionine (Met) and beta,gamma-imido-ATP. In its synthesis the N-Boc derivative of 5'(R)-C-(aminomethyl)-N6-benzoyl-5'-O-tosyl-2',3'-O- isopropylidenadenosine was converted by the successive actions of CF3CO2H and HNO2 into the corresponding 5'(R)-C-hydroxymethyl derivative. Treatment of this with disodium L-homocysteinate led to attack of sulfur at C6', apparently via a 5',6'-epoxide, and to total stereoselective inversion at C5' to furnish, after debenzoylation, 5'(R)-C-(L-homocystein-S-ylmethyl)-2',3'-O-isopropylidene ade nosine. The 5' configuration was established by conversion of this into the known 5'(S)-C-methyl-2',3'-O-isopropylidene adenosine with Raney nickel. The alpha-amino acid residue was protected as an N-Boc methyl ester, after which the 5'-hydroxyl was phosphorylated with benzyl phosphate and dicyclohexylcarbodiimide. The phosphoanhydride bond with inorganic imidodiphosphate was then created by established methods. Finally, blocking groups were removed under conditions that gave the desired adduct with no racemization of its L-methionine residue. It was a potent inhibitor [KM(ATP)/Ki = 1080; KM(Met)/Ki = 7.7] of the M-2 (normal tissue) form of rat methionine adenosyltransferase and of the M-T (hepatoma tissue) form [KM(ATP)/Ki = 670; KM(Met)/Ki = 22]. Inhibitions were competitive with respect to ATP or to L-methionine, indicating a dual substrate site mode of binding to the enzyme forms.
Sensitivity of Screening Tests for Detecting Vision In Preschoolers-targeted Vision Disorders When Specificity Is 94% (2005)

Similar to the previously reported results at 90% specificity, the screening tests vary widely in sensitivity with specificity set at 94%. The rankings of the sensitivities for detection of the 4 VIP-targeted vision disorders are similar to those with specificity set to 90%.
Wax precipitation from North Sea crude oils. 3. Precipitation and dissolution of wax studied by differential scanning calorimetry (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTWax precipitation from North Sea crude oils. 3. Precipitation and dissolution of wax studied by differential scanning calorimetryAsger Baltzer Hansen, Elfinn Larsen, Walther Batsberg Pedersen, Anne B. Nielsen, and Hans Petter RoenningsenCite this: Energy Fuels 1991, 5, 6, 914–923Publication Date (Print):November 1, 1991Publication History Published online1 May 2002Published inissue 1 November 1991https://pubs.acs.org/doi/10.1021/ef00030a021https://doi.org/10.1021/ef00030a021research-articleACS PublicationsRequest reuse permissionsArticle Views892Altmetric-Citations122LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A transparent MAC method for bandwidth sharing and CDV control at the ATM layer of passive optical networks (1996)

In the quest for a way to bring local loop costs down and make B-ISDN affordable to small traffic customers, the passive optical networks (PONs) in combination with ATM (APONs) have been investigated extensively. To achieve the required medium sharing on a time division multiplexing (TDMA) basis, an arbitration mechanism is required in the upstream direction. The resulting multiplexing and concentration, though it is the source of significant savings, it has been plagued by the penalty of high distortion of the traffic profiles at the exit of the PON. The preventive congestion control method of ATM is rather intolerant of such distortions in the multiplexing stages. Arbitration methods which are either semistatic or based on number of arrivals per reservation period, cause very high cell delay variation (CDV). The cell clustering introduced by the arbitration method cannot be distinguished from peak rate violations of the contract by the policing unit. The result is either unwarranted rejections of conforming cells or unacceptably low utilization. The scheme presented herewith maintains a CDV behavior comparable to that of a common centralized FIFO multiplexer. It relies on a meticulous recording of the arrival timing of cells enabling a subsequent allocation of slots which produces almost the same output (and CDV) as the centralized FLFO multiplexer. A compact coding of the timing information restricts bandwidth waste for control information to the size found in other similar protocols. The most important feature is that the scheme is amenable to easy hardware implementation and does not require any call related information.
Effect of the iliotibial band on knee biomechanics during a simulated pivot shift test (2006)

The purpose of this study was to evaluate the effect of the iliotibial band (ITB) on the kinematics of anterior cruciate ligament (ACL) intact and deficient knees and also on the in situ force in the ACL during a simulated pivot shift test. A combination of 10 N-m valgus and 5 N-m internal tibial torques was applied to 10 human cadaveric knees at 15 degrees, 30 degrees, 45 degrees, and 60 degrees of flexion using a robotic/universal force-moment sensor testing system. ITB forces of 0, 22, 44, and 88 N were also applied. An 88 N ITB force significantly decreased coupled anterior tibial translation of ACL deficient knees by 32%-45% at high flexion angles, but did not have a significant effect at low flexion angles. Further, an 88 N ITB force significantly decreased in situ forces in the ACL at all flexion angles by 23%-40%. These results indicate that during the pivot shift test, the ITB can improve tibial reduction at high flexion angles while not affecting subluxation at low flexion angles. Additionally, the action of the ITB as an ACL agonist suggests that its use as an ACL graft might hinder knee stability in response to rotatory load.
Crenolanib Inhibits the Drug-Resistant PDGFRA D842V Mutation Associated with Imatinib-Resistant Gastrointestinal Stromal Tumors (2012)

Crenolanib is a potent inhibitor of imatinib-resistant PDGFRA kinases associated with GIST, including the PDGFRA D842V mutation found in approximately 5% of GISTs. The spectrum of activity of crenolanib suggests that this drug is a type I inhibitor (inhibitor of activated conformation of kinase). Based in part on these results, a phase II clinical study of this agent to treat GIST with the PDGFRA D842V mutation has been initiated.
Using machine learning for unsupervised maritime waypoint discovery from streaming AIS data (2015)

Estimating the future position of a deep sea vessel more than 24 hours in advance is a major challenge for Dutch logistics service providers (LSPs). Their unscheduled arrival in ports directly impacts scheduling and waiting times of barges, propagating throughout the entire supply chain network. To help LSPs' planners improve planning operations, we intend to capture the characteristics of maritime routes for a specific region (the North Sea connecting the Netherlands and United Kingdom) in the form of a directed graph, which can be used as a foundation for predicting destination and arrival time of each associated vessel. To create such graph we need an efficient way to extract waypoints for traffic data and this is the problem we will address in this paper.
Adaptation of a CCR5-Using, Primary Human Immunodeficiency Virus Type 1 Isolate for CD4-Independent Replication (1999)

The gp120 envelope glycoprotein of the human immunodeficiency virus type 1 (HIV-1) promotes virus entry by sequentially binding CD4 and chemokine receptors on the target cell. Primary, clinical HIV-1 isolates require interaction with CD4 to allow gp120 to bind the CCR5 chemokine receptor efficiently. We adapted a primary HIV-1 isolate, ADA, to replicate in CD4-negative canine cells expressing human CCR5. The gp120 changes responsible for the adaptation were limited to alteration of glycosylation addition sites in the V2 loop-V1-V2 stem. The gp120 glycoproteins of the adapted viruses bound CCR5 directly, without prior interaction with CD4. Thus, a major function of CD4 binding in the entry of primary HIV-1 isolates can be bypassed by changes in the gp120 V1-V2 elements, which allow the envelope glycoproteins to assume a conformation competent for CCR5 binding.
Bacterial and Fungal Microbiota of Guinea Grass Silage Shows Various Levels of Acetic Acid Fermentation (2021)

This study aimed to gain insights into the bacterial and fungal microbiota associated with the acetic acid fermentation of tropical grass silage. Direct-cut (DC, 170 g dry matter [DM]/kg) and wilted (WT, 323 g DM/kg) guinea grass were stored in a laboratory silo at moderate (25 °C) and high (40 °C) temperatures. Bacterial and fungal microbiota were assessed at 3 days, 1 month, and 2 months after ensiling. Lactic acid was the primary fermentation product during the initial ensiling period, and a high Lactococcus abundance (19.7–39.7%) was found in DC silage. After two months, the lactic acid content was reduced to a negligible level, and large amounts of acetic acid, butyric acid, and ethanol were found in the DC silage stored at 25 °C. The lactic acid reduction and acetic acid increase were suppressed in the DC silage stored at 40 °C. Increased abundances of Lactobacillus, Clostridium, and Wallemia, as well as decreased abundances of Saitozyma, Papiliotrema, and Sporobolomyces were observed in DC silages from day three to the end of the 2 month period. Wilting suppressed acid production, and lactic and acetic acids were found at similar levels in WT silages, regardless of the temperature and storage period. The abundance of Lactobacillus (1.72–8.64%) was lower in WT than in DC silages. The unclassified Enterobacteriaceae were the most prevalent bacteria in DC (38.1–64.9%) and WT (50.9–76.3%) silages, and their abundance was negatively related to the acetic acid content. Network analysis indicated that Lactobacillus was involved in enhanced acetic acid fermentation in guinea grass silage.