gebhart/patrepeval huggingface dataset:
Stimulation of protein synthesis in COS cells transfected with variants of the alpha-subunit of initiation factor eIF-2. (1992)

The role of eukaryotic initiation factor 2 (eIF-2) phosphorylation in translational control has been demonstrated in vivo by overexpressing variant forms of eIF-2 alpha that are not phosphorylated. COS-1 cells transiently transfected with expression vectors for human eIF-2 alpha contain 10-20-fold more eIF-2 alpha subunit than the endogenous COS cell eIF-2 trimeric complex. Expression of the variant form of eIF-2 alpha, Ser51Asp, where Asp replaces Ser51, causes inhibition of protein synthesis, whereas the Ser48Asp variant does not. When either Ser48 or Ser51 is replaced by Ala, the variants stimulate dihydrofolate reductase synthesis when the eIF-2 alpha kinase, DAI, is activated. In order to elucidate these mechanisms, we have separated eIF-2 trimeric complexes from free overexpressed eIF-2 alpha subunits by fast protein liquid chromatography Superose chromatography. Pulse-labeled cells transfected with wild-type or variant DNAs produced eIF-2 preparations with greater than 10-fold higher specific radioactivity in the alpha-subunit compared to the gamma-subunit, thus demonstrating that the human eIF-2 alpha produced from the plasmids readily exchanges into COS cell eIF-2 complexes. Both wild-type and Ser48Ala variant forms of the free 2 alpha-subunit, further purified by MonoQ chromatography, are poor substrates for the heme-regulated eIF-2 alpha kinase, HRI, but are good substrates for double-stranded RNA-activated inhibitor in vitro; the Ser51Ala variant subunit is not phosphorylated by either kinase. None of the purified free eIF-2 alpha subunits inhibits phosphorylation of eIF-2 in vitro, even at up to 8-fold molar excess. Examination of the extent of eIF-2 alpha phosphorylation in the COS cell eIF-2 complexes by two-dimensional polyacrylamide gel electrophoresis shows that the stimulation of dihydrofolate reductase synthesis by the Ser51Ala variant is most readily explained by failure of eIF-2 to be phosphorylated. Stimulation by the Ser48Ala variant appears to occur by mitigation of the effect of phosphorylation at Ser51 since the double variant, Ser48Ala-Ser51Asp, inhibits protein synthesis less than the single variant Ser51Asp. The evidence argues strongly against there being a second site of phosphorylation involved in translational repression.
Concurrent Zinc-Blende and Wurtzite Film Formation by Selection of Confined Growth Planes (2018)

Recent research on nanowires (NWs) demonstrated the ability of III-V semiconductors to adopt a different crystallographic phase when they are grown as nanostructures, giving rise to a novel class of materials with unique properties. Controlling the crystal structure however remains difficult and the geometrical constraints of NWs cause integration challenges for advanced devices. Here, we report for the first time on the phase-controlled growth of micron-sized planar InP films by selecting confined growth planes during template-assisted selective epitaxy. We demonstrate this by varying the orientation of predefined templates, which results in concurrent formation of zinc-blende (ZB) and wurtzite (WZ) material exhibiting phase purities of 100% and 97%, respectively. Optical characterization revealed a 70 meV higher band gap and a 2.5× lower lifetime for WZ InP in comparison to its natural ZB phase. Further, a model for the transition of the crystal structure is presented based on the observed growth facets and the bonding configuration of InP surfaces.
A CONCEPTUAL CELL FOR ELECTROWINNING LIQUID URANIUM. (1969)

A production-scale cell is proposed for electrowinning liquid uranium from uranium dioxide feed in a molten fluoride electrolyte. The design is based on the developement work of several investigators. Features of the design include a vertical cathode, a terraced, electrically floating hearth, isolation of the metal pool from the oxide feed, and provision for rapid removal of the molten metal. The paper analyzes problems of other researchers in achieving high current efficiencies and the importance in the cell design of feeding sufficient oxide to the anode area and of maintaining ample metal-to-anode spacing.
Porous−wall BeO capillary waveguide laser (1975)

Small−bore capillary tubes exhibit very high optical gains and saturation intensities in flowing gas CO2 systems. However, these parameters may vary greatly in the axial direction due to the nonuniform axial pressure distribution. Use of porous−wall BeO capillary tubes makes it possible to attain a more uniform pressure distribution and to usefully scale capillary waveguide amplifiers to longer lengths.
The modern European state and system of states (2002)

This unique collection presents texts in international relations from Ancient Greece to the First World War. Major writers such as Thucydides, Augustine, Aquinas, Machiavelli, Grotius, Kant and John Stuart Mill are represented by extracts of their key works; less well-known international theorists including John of Paris, Cornelius van Bynkershoek and Friedrich List are also included. Fifty writers are anthologised in what is the largest such collection currently available. The texts, most of which are substantial extracts, are organised into broadly chronological sections, each of which is headed by an introduction that places the work in its historical and philosophical context. Ideal for both students and scholars, the volume also includes biographies and guides to further reading.
Cluster-Suppressed Plasma Chemical Vapor Deposition Method for High Quality Hydrogenated Amorphous Silicon Films (2002)

We have developed a novel plasma chemical vapor deposition (PCVD) method for preparing high quality hydrogenated amorphous silicon (a-Si:H) films, which suppresses effectively growth of clusters by transporting them out of the reactor using gas flow and thermophoresis. By utilizing this cluster-suppressed PCVD method, we have demonstrated deposition of quite high quality a-Si:H films, microstructure parameter Rα of which can be reduced below 0.003. The decrease in Rα value is closely related to the decrease in cluster amount. Preliminary evaluation of fill factor (FF) of the a-Si:H Schottky solar cell of the a-Si:H films of Rα=0.057 shows the high initial value FFi=0.57 and high stabilized value after-light-soaking FFa=0.53.
Image reconstruction from frequency-offset Fourier data (1984)

Motivated by the ability of synthetic-aperture radar and related imaging systems to produce images of surprisingly high quality, we consider the problem of reconstructing the magnitude of a complex signal f from samples of the Fourier transform of f located in a small region offset from the origin. It is shown that high-quality speckle reconstructions are possible so long as the phase of f is highly random. In this case, the quality of the reconstruction is insensitive to the location of the known Fourier data, and edges at all orientations are reproduced equally well. A large number of computer examples are presented demonstrating these attributes. Methods for improving image quality are also briefly discussed.
The Effectiveness of Antihistamines in the Common Cold (1981)

A randomized, double-blind, placebo-controlled study was done on 106 volunteers to assess the effectiveness of chlorpheniramine maleate against the common cold. The results suggest that this antihistamine is significantly effective in relieving the symptoms of a cold if used four times daily for one week, and that relief was gained with no significant increase in side effects.
Nerve growth factor receptors on cultured rat Schwann cells (1988)

Neonatal rat Schwann cells were grown in tissue culture and assayed for NGF receptors with time in culture. NGF receptor levels on freshly prepared Schwann cells (day 0) were low but increased dramatically during the first week in culture. Characterization of 125I-NGF binding to resuspended cells grown for 4 d in culture revealed that binding was not saturable at high ligand concentrations (50-70 nM) and that a high-capacity, low-affinity NGF binding component existed on these cells as compared to PC12 cells. The monoclonal antibody, 192-IgG, which recognizes the rat NGF receptor, was used as an immunohistochemical tool to verify the presence of NGF receptors on the cultured rat Schwann cells. In contrast to radiolabeled NGF, 125I-192-IgG demonstrated saturable binding to Schwann cells in suspension, with Kd and Bmax values of 4 nM and 115 fmol/10(6) cells, respectively. Schwann cells showed no evidence of slow dissociation or internalization of NGF binding at any of several NGF concentrations. 192-IgG was used to immunoprecipitate 125I-NGF chemically crosslinked to cell membranes. SDS-PAGE and subsequent autoradiography of the immunoprecipitated NGF receptors revealed that 2 species of NGF receptors were precipitated from Schwann cells and PC12 cells. In PC12 cells, 2 bands with molecular weights of 90 and 210 kDa were identified. The Schwann cell NGF receptor species migrated slower on the gels, with apparent molecular weights of 95 and 220 kDa. Further analysis of glial cell NGF receptors showed that Schwann cells isolated from the vagus nerve of neonatal rats also expressed NGF receptors in culture; however, astrocytes cultured from neonatal rat cerebral cortex, cultured under conditions reported here, were devoid of detectable NGF receptors. These results show that NGF receptor levels on Schwann cells increase with time in culture, and this resembles what is observed in Schwann cells in vivo when adult peripheral nerve is injured. The data are discussed in terms of a supportive role for the Schwann cell in facilitating peripheral nerve development and regeneration.
Recombinant full-size human antibody to Ebola virus (2007)

A full-size human antibody to Ebola virus was constructed by joining genes encoding the constant domains of the heavy and light chains of human immunoglobulin with the corresponding DNA fragments encoding variable domains of the single-chain antibody 4D1 specific to Ebola virus, which was chosen from a combinatorial phage display library of single-strand human antibodies. Two expression plasmids. pCH1 and pCL1, containing the artificial genes encoding the light and heavy chains of human immunoglobulin, respectively, were constructed. Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody. The affinity constant for the antibody was estimated by solid-phase enzyme-linked immunoassay to be 7.7 x 10(7) +/- 1.5 x 10(7) M(-1). Like the parent single-chain antibody 4DI, the resulting antibody bound the nucleoprotein of Ebola virus and did not interact with the proteins of Marburg virus.
Perturbed Angular Correlation Study of a Haptenic Molecule (1972)

The angular correlation of the 173-247 keV gamma-ray cascade after the electron-capture decay of (111)In is strongly perturbed when the 1-p-nitrophenyl-ethylenediaminetetraacetate chelate of (111)In(8+) is added to a solution containing rabbit antibody to dinitrophenyl groups. The radioactive chelate can be displaced by the addition of dinitrophenyllysine or unlabeled chelate. The average association constant between the antibody and the labeled chelate has been estimated from perturbed angular correlation measurements; this value is compared to the results of equilibrium dialysis. These experiments provide good evidence that information concerning macromolecular behavior can be obtained from perturbed angular correlation experiments that use chemically specific labels.
HRM-facilitated rapid identification and genotyping of mutations induced by CRISPR/Cas9 mutagenesis in rice (2018)

The clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated (Cas) technology has recently emerged as a powerful genomic editing tool with great potential for crop breeding. However, commonly used protocols for screening of CRISPR/Cas9-induced mutations are laborious, timeconsuming, and costly. In the present study we examined the applicability of high resolution melting (HRM) analysis fast screening CRISPR/Cas9-induced mutations in T 0 plants and subsequent genotyping of T 1 populations. Comparative analysis demonstrated that HRM analysis could identify mutant T 0 plants carrying various types of mutation, including single nucleotide substitutions and short insertion/deletions, with false positive/negative rates of 0-2.78%. Furthermore, T 1 plants derived from single T 0 plants could be correctly genotyped by HRM analysis using WT parents and T 0 plants as controls. We hence recommend the adoption of HRM analysis in CRISPR/Cas9 mediated genetic studies and breeding in rice and other crop species.
Global AIDS into the 1990s. (1990)

(1990). Global AIDS into the 1990s. Interdisciplinary Science Reviews: Vol. 15, No. 2, pp. 110-112.
Reduced Graphene Oxide Nanosheet for Chemo-photothermal Therapy (2016)

The protein-functionalized reduced graphene oxide (rGO) nanosheet is of great interest in stimuli-responsive drug delivery and controlled release applications. We developed doxorubicin (DOX)-loaded bovine serum albumin (BSA)-functionalized rGO (DOX-BSA-rGO) nanosheets. To investigate the reduction of BSA-functionalized GO nanosheets and drug loading efficiency, we used X-ray photoelectron spectroscopy (XPS) and UV-visible spectrophotometer analysis. DOX-BSA-rGO nanosheets exhibited dose-dependent cellular uptake without any cytotoxic effect. We also demonstrated near-infrared (NIR)-induced chemo-photothermal therapy of brain tumor cells treated with DOX-BSA-rGO nanosheets. Therefore, this DOX-BSA-rGO nanosheet could be a powerful tool for chemo-photothermal therapy applications.
Signature verification with a syntactic neural net (1990)

A syntactic neural network is equivalent to a parser for a certain type of grammar-in this case, strictly hierarchical context-free. This allows an efficient method for pattern description and has the added advantage of being a generative model. The authors show how the network itself can infer the grammar. Syntactic neural nets can model stochastic or nonstochastic grammars. The stochastic nets are properly probabilistic and are powerful discriminators; the nonstochastic nets are less powerful, but have straightforward silicon implementations with existing technology. Learning in syntactic nets may proceed supervised or unsupervised. In each case, the algorithm is the same; the difference lies in the data presented to the net. In prior publications, the authors applied syntactic neural nets to character recognition and cursive script recognition. The authors presently show that nonstochastic nets can perform signature verification with high reliability. This raises the possibility of signature verification on a robust smart card
Automated Playtesting With Procedural Personas Through MCTS With Evolved Heuristics (2018)

This paper describes a method for generative player modeling and its application to the automatic testing of game content using archetypal player models called procedural personas. Theoretically grounded in psychological decision theory, procedural personas are implemented using a variation of Monte Carlo tree search (MCTS) where the node selection criteria are developed using evolutionary computation, replacing the standard UCB1 criterion of MCTS. Using these personas, we demonstrate how generative player models can be applied to a varied corpus of game levels and demonstrate how different playstyles can be enacted in each level. In short, we use artificially intelligent personas to construct synthetic playtesters. The proposed approach could be used as a tool for automatic play testing when human feedback is not readily available or when quick visualization of potential interactions is necessary. Possible applications include interactive tools during game development or procedural content generation systems where many evaluations must be conducted within a short time span.
<i>GLI</i> <i>3</i> mutations in human disorders mimic <i>Drosophila</i> Cubitus interruptus protein functions and localization (1999)

Truncation mutations of the GLI3 zinc finger transcription factor can cause Greig cephalopolysyndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), and postaxial polydactyly type A (PAP-A). GLI3 is homologous to Drosophila Cubitus interruptus (Ci), which regulates the patched (ptc), gooseberry (gsb), and decapentaplegic (dpp) genes. Ci is sequestered in the cytoplasm and is subject to posttranslational processing whereby the full-length transcriptional activator form (Ci155) can be cleaved to a repressor form (Ci75). Under hedgehog signaling, the Ci155 form translocates to the nucleus whereas in the absence of hedgehog, the Ci75 form translocates to the nucleus. Based on the correlation of GLI3 truncation mutations and the human phenotypes, we hypothesized that GLI3 shows transcriptional activation or repression activity and subcellular localization similar to Ci. Here we show that full-length GLI3 localizes to the cytoplasm and activates PTCH1 expression, which is similar to full-length Ci155. PHS mutant protein (GLI3-PHS) localizes to the nucleus and represses GLI3-activated PTCH1 expression, which is similar to Ci75. The GCPS mutant protein has no effect on GLI3-activated PTCH1 transcription, consistent with the role of haploinsufficiency in this disorder. The PAP-A mutant protein (GLI3-PAP-A) showed less specific subcellular localization but still inhibited GLI3-activated PTCH1 transcription, suggesting it may be a weaker allele than the GLI3-PHS mutation. These data show that GLI3 mutations in humans mimic functional effects of the Drosophila ci gene and correlate with the distinct effects of these mutations on human development.
Noise removal by migration of time-shift images (2014)

ABSTRACT When using seismic data to image complex structures, the reverse time migration (RTM) algorithm generally provides the best results when the velocity model is accurate. With an inexact model, moveouts appear in common image gathers (CIGs), which are either in the surface offset domain or in subsurface angle domain; thus, the stacked image is not well focused. In extended image gathers, the strongest energy of a seismic event may occur at non-zero-lag in time-shift or offset-shift gathers. Based on the operation of RTM images produced by the time-shift imaging condition, the non-zero-lag time-shift images exhibit a spatial shift; we propose an approach to correct them by a second pass of migration similar to zero-offset depth migration; the proposed approach is based on the local poststack depth migration assumption. After the proposed second-pass migration, the time-shift CIGs appear to be flat and can be stacked. The stack enhances the energy of seismic events that are defocused at zero time lag due to the inaccuracy of the model, even though the new focused events stay at the previous positions, which might deviate from the true positions of seismic reflection. With the stack, our proposed approach is also able to attenuate the long-wavelength RTM artifacts. In the case of tilted transverse isotropic migration, we propose a scheme to defocus the coherent noise, such as migration artifacts from residual multiples, by applying the original migration velocity model along the symmetry axis but with different anisotropic parameters in the second pass of migration. We demonstrate that our approach is effective to attenuate the coherent noise at subsalt area with two synthetic data sets and one real data set from the Gulf of Mexico.
Silicon carbide color centers for quantum applications (2020)

Abstract Silicon carbide has recently surged as an alternative material for scalable and integrated quantum photonics, as it is a host for naturally occurring color centers within its bandgap, emitting from the UV to the IR even at telecom wavelength. Some of these color centers have been proved to be characterized by quantum properties associated with their single-photon emission and their coherent spin state control, which make them ideal for quantum technology, such as quantum communication, computation, quantum sensing, metrology and can constitute the elements of future quantum networks. Due to its outstanding electrical, mechanical, and optical properties which extend to optical nonlinear properties, silicon carbide can also supply a more amenable platform for photonics devices with respect to other wide bandgap semiconductors, being already an unsurpassed material for high power microelectronics. In this review, we will summarize the current findings on this material color centers quantum properties such as quantum emission via optical and electrical excitation, optical spin polarization and coherent spin control and manipulation. Their fabrication methods are also summarized, showing the need for on-demand and nanometric control of the color centers fabrication location in the material. Their current applications in single-photon sources, quantum sensing of strain, magnetic and electric fields, spin-photon interface are also described. Finally, the efforts in the integration of these color centers in photonics devices and their fabrication challenges are described.
Compact single-etched sub-wavelength grating couplers for O-band application (2017)

We demonstrate two single-etched sub-wavelength grating coupler (SWGC) designs for O-band application, one targeting at high coupling efficiency and the other targeting at broad operating bandwidth. The high-efficiency SWGC has a measured peak coupling efficiency of -3.8 dB and a 3-dB bandwidth of 40 nm, and the broadband SWGC has a measured peak coupling efficiency of -4.3 dB and a 3-dB bandwidth of 71 nm. Focusing gratings have been used in our SWGCs to reduce the design footprints and the dimensions of our SWGCs are smaller than 45 μm × 24 μm. The back reflections of our SWGCs are suppressed to be below -15 dB over the wavelength range from 1260 nm to 1360 nm.
Piecewise linear skeletonization using principal curves (2002)

Proposes an algorithm to find piecewise linear skeletons of handwritten characters by using principal curves. The development of the method was inspired by the apparent similarity between the definition of principal curves (smooth curves which pass through the "middle" of a cloud of points) and medial axes (smooth curves that run equidistantly from the contours of a character image). The central fitting-and-smoothing step of the algorithm is an extension of the polygonal line algorithm, which approximates principal curves of data sets by piecewise linear curves. The polygonal line algorithm is extended to find principal graphs and complemented with two steps specific to the task of skeletonization: an initialization method to capture the approximate topology of the character, and a collection of restructuring operations to improve the structural quality of the skeleton produced by the initialization method. An advantage of our approach over existing methods is that we optimize the skeleton graph by minimizing an intuitive and explicit objective function that captures the two competing criteria of smoothing the skeleton and fitting it closely to the pixels of the character image. We tested the algorithm on isolated handwritten digits and images of continuous handwriting. The results indicated that the proposed algorithm can find a smooth medial axis in the great majority of a wide variety of character templates and that it substantially improves the pixel-wise skeleton obtained by traditional thinning methods.