gebhart/patrepeval huggingface dataset:
Mechanism of selective translation of vaccinia virus mRNAs: differential role of poly(A) and initiation factors in the translation of viral and cellular mRNAs (1991)

We have recently demonstrated that the poly(A) moieties of short RNAs obtained from both in vitro transcription and from vaccinia virus (VV)-infected cells exhibit dissimilar effects on the in vitro translation of cellular and VV mRNAs (R. Bablanian, G. Coppola, P. Masters, and A. K. Banerjee, Virology 148:375-380, 1986; M. J. Su and R. Bablanian, Virology 179:679-693, 1990). In the present study, we have investigated the roles of poly(A), m7GTP, and initiation factors in the mechanism of selective translation of VV mRNAs. The effects of unfractionated poly(A) [termed poly(A)un, with various chain lengths up to 3,000 nucleotides] and a 150- to 300-nucleotide fraction of synthetic poly(A) [termed poly(A)150-300] on the translation of HeLa cell mRNAs and early and late VV mRNAs were studied. Both the poly(A)un and the poly(A)150-300 completely inhibited the translation of HeLa cell mRNAs obtained from total cytoplasmic RNA in the nuclease-treated reticulocyte lysates. Viral mRNAs from total cytoplasmic RNA also were slightly inhibited (15 to 38%) by the poly(A)un, whereas the poly(A)150-300 had no significant effect on their translation. The translation of oligo(dT)-cellulose-selected HeLa mRNAs was as sensitive to inhibition by poly(A)150-300 as the mRNAs found in total cytoplasmic RNA. However, the translations of oligo(dT)-cellulose-selected viral mRNAs become more sensitive to the inhibitory effect of poly(A)150-300 than the translations of viral mRNAs found in the total cytoplasmic RNA. Both HeLa and VV mRNAs became more resistant to the poly(A)-mediated inhibition when these mRNAs were deadenylated, but the relative resistance to inhibition by poly(A)150-300 of deadenylated VV mRNAs was much greater than that of HeLa cell mRNAs. The translation of VV mRNAs was significantly less inhibited than the translation of HeLa mRNAs when the cap analog, m7GTP, was added to the cell-free system. The inhibition of HeLa cell mRNA translation by both poly(A)un and poly(A)150-300 was completely restored when poly(A)-binding protein (PAB) was added to the cell-free translational system. The addition of eukaryotic initiation factor 4A (eIF-4A) did not restore translation when poly(A)un was used to inhibit translation; however, inhibition by poly(A)150-300 was significantly reversed by this initiation factor. The reversal of poly (A)-mediated inhibition of HeLa cell mRNA translation was additive when PAB was used together with eIF-4A. Early VV mRNA translation was only slightly inhibited by poly(A)un (15%), and this inhibition was completely reversed by either PAB or eIF-4A.(ABSTRACT TRUNCATED AT 400 WORDS)
DivS, a novel SOS‐inducible cell‐division suppressor in <i>Corynebacterium glutamicum</i> (2007)

DNA damage-induced SOS response elicits the induction of cell-division suppressor as well as DNA repair genes. In Gram-positive bacteria, cell-division suppressor genes, so far characterized from Bacillus subtilis (yneA) and Mycobacterium tuberculosis (rv2719c), share limited homology, but are both located in the vicinity of lexA on their respective genomes. Using this proximity to lexA, Corynebacterium glutamicum R divS (cgR1759) was identified as an SOS-inducible cell-division suppressor in this study. The amino acid sequence of DivS showed no homology to that of YneA and Rv2719c. divS expression was markedly induced by DNA-damaging mitomycin C treatment in wild-type cells, but not in its DeltarecA mutant cells, which are unable to induce the SOS response. Wild-type C. glutamicum R cells exposed to DNA-damaging mitomycin C exhibited elongated morphology that, using green fluorescent protein-FtsZ fusion protein, was attributed to defects in FtsZ ring assembly. Cells defective in FtsZ ring assembly were subsequently incapable of septum wall synthesis. In the presence of mitomycin C, divS mutant cells did not exhibit this elongated morphology, whereas cells overexpressing divS were elongated and abnormally branched.
Component replication in distributed systems: a case study using Enterprise Java Beans (2004)

A recent trend has seen the extension of object-oriented middleware. A major advantage components offer over objects is that only the business logic of an application needs to be addressed by a programmer with support services required incorporated into the application at deployment time. This is achieved via components (business logic of an application), containers that host components and are responsible for providing the underlying middleware services required by components and application servers that host containers. Well-known examples of component middleware architectures are Enterprise Java Beans (EJBs) and the CORBA Component model (CCM). Two of the many services available at deployment time in most component architectures are component persistence and atomic transactions. This paper examines, using EJBs, how replication for availability can be supported by containers so that components that are transparently using persistence and transactions can also be made highly available.
Creep crack growth in cold-formed C–Mn steel at 360°C (1987)

AbstractAn investigation of subcritical crack growth in C–Mn steels at 360°C has been conducted to provide data relevant to the assessment of the long-term integrity of cold-formed bends in boiler integral pipework systems. The material was obtained from bends that exhibited hardnesses towards the top and bottom of the usually observed range and which had been in service for approximately 63000 h. Crack growth rates were found to be a function of specimen displacement rates, and despite evidence that growth may have occurred under non-steady-state conditions, good correlation of the data was obtained using the C* parameter. Material hardness was found to be a major factor in determining resistance to cracking, although the trends observed in creep and crack growth properties suggest that its effect may be reduced at long exposure times.MST/509
Effects of free fatty acids, growth hormone and growth hormone receptor blockade on serum ghrelin levels in humans (2007)

Elevation of FFAs by means of an intravenous infusion acutely suppresses ghrelin levels, whereas GH administration and GHR blockade have no detectable effect on ghrelin concentration when insulin and FFA levels are kept fixed.
Overexpression of LIMK1 Promotes Migration Ability of Multidrug-Resistant Osteosarcoma Cells (2011)

Multidrug resistance (MDR) to chemotherapy is a major obstacle in the treatment of cancer and the resistance process is multifactorial. Studies on multidrug resistance mechanisms relied on the availability of cancer multidrug resistance cell lines that have been established. In this study we successfully established a multidrug resistance cell line MG63/VCR derived from human osteosarcoma cell line MG63 based on the induction by vincristine. MG63/VCR cells exhibited high resistance to vincristine and other anticancer drugs, accompanied by upregulated expression of MDR-associated genes MDR1, MRP1, and Bcl-2. Notably, we found that MG63/VCR cells exhibited higher migration ability compared to parental MG63 cells. Moreover, we demonstrated that LIMK1, a key regulator of actin cytoskeleton, was overexpressed at both mRNA and protein levels in MG63/VCR cells and the higher LIMK1 protein level was correlated with higher level of phosphorylated cofilin. In addition, knockdown of LIMK1 abolished the higher migration ability of MG63/ VCR cells. These results suggest that LIMK1 overexpression contributes to the invasion and metastasis of drug-resistant osteosarcoma and reveal LIMK as a novel therapeutic target for drug resistant osteosarcoma.
Diffusion in Ethylene Polymers. IV (1959)

Self-diffusion coefficients have been measured for two low molecular weight fractions (M = 4100 and 5800) of a linear polyethylene. The activation energy for the M = 4100 fraction is 5.3 kcal/mole which is near the value 5.6 kcal/mole found for n — C32H66. At 150°C, for this fraction, D = 1.6×10—7 cm2/sec and as 10—7 is near the lower limit of detectability the activation energy is probably not accurately determined. The diffusion coefficient for the M = 5800 fraction is 1.1×10—7 cm2/sec at 150°C. These results, combined with previous results, show that D = κn—5/3, where D is the coefficient of self-diffuison for the normal paraffin CnH2n+2. κ is a constant at a given temperature, 4.5×10—3 cm2/sec at 200°C and 2.5×10—3 cm2/sec at 150°C.
Influence of the Anodic Work Function on the Performance of Organic Solar Cells (2002)

Cutting etch organic solar cells utilize the bulk-heterojunction concept, shown in the graphic, of interpenetrating electron and hole conducting networks which enhances the exciton splitting process in the photosensitive layer. Generated charges are turned into current due to the built-in field given by the adjoining electrodes. Here, the influence of an altered doping level of the hole collecting electrode has been investigated.
Calcium supplements with or without vitamin D and risk of cardiovascular events: reanalysis of the Women's Health Initiative limited access dataset and meta-analysis (2011)

Calcium supplements with or without vitamin D modestly increase the risk of cardiovascular events, especially myocardial infarction, a finding obscured in the WHI CaD Study by the widespread use of personal calcium supplements. A reassessment of the role of calcium supplements in osteoporosis management is warranted.
When the proton becomes larger (2011)

The TOTEM experiment at the LHC has just confirmed that, at high energy, protons behave as if they were becoming larger. In more technical terms, their total cross-section – a parameter linked to the proton-proton interaction probability – increases with energy. This phenomenon, expected from previous measurements performed at much lower energy, has now been confirmed for the first time at the LHC’s unprecedented energy. One arm of a TOTEM T2 detector during its installation at interaction point 5. A composite particle like the proton is a complex system that in no way resembles a static Lego construction: sub-components move inside and interactions keep the whole thing together, but in a very dynamic way. This partly explains why even the very common proton can still be hiding secrets about its nature, decades after its discovery. One way of studying the inner properties of protons is to observe how they interact with each other, which, in technical terms, implies calculating the total cross-section of the proton-proton interactions. Early measurements at the CERN ISR surprisingly showed that the cross-section increases when the energy increases. This was then confirmed by the CERN SppS Collider and the Tevatron. But this is the first time that the trend has been confirmed at the highest energy, that of the LHC. “TOTEM’s result of (98 ± 3) mbarn for the total cross-section confirms that, even at the so far unexplored energy of the LHC, the proton behaves as if it were becoming larger,” says Karsten Eggert, spokesperson of the TOTEM collaboration. TOTEM measurement of the total cross-section of the proton at the 7 TeV centre-of-mass energy: 98 ± 3 mbarn. Measuring the proton-proton total cross-section is not a trivial exercise. “We requested a special run of the LHC,” explains Eggert. “The beam divergence in the proximity of the interaction points in the machine had to be much smaller than in standard LHC operation. In only thirty minutes of data taking with this special beam configuration, TOTEM collected sufficient data to measure the elastic proton-proton scattering cross-section, which made it possible to determine the total cross-section using the so-called optical theorem." The present calculation is based on the luminosity measurement provided by the CMS experiment, but the collaboration’s future plans involve using only TOTEM’s detectors to measure it in a luminosity-independent way. “Rather soon we will have a longer specialised run and move our detectors closer to the beams,” says Eggert. TOTEM’s new result will significantly contribute to a deeper understanding of the nature of the proton. From theory, we know that there is a limit to how large the proton can become if pushed to the highest energies. “For the time being, our results are beautifully in agreement with data coming from cosmic rays and extrapolations made from previous measurements. This result represents the first experimental confirmation of long-standing guesses that physicists made about the proton’s behaviour at high-energy,” concludes Eggert.
Novel drain-biased transconductance building blocks for continuous-time filter applications (1989)

A family of continuous-time RC filter building blocks is introduced which uses the linear transconductance of an MOS transistor operating in the triode region as a tunable element. The conductance is tuned by controlling the transistor's drain voltage through a bias current. This technique offers a wide input linear range, good frequency behaviour, ease of tuning and high input impedance.
Treatment of Infection Due to <i>Pneumocystis carinii</i> (1998)

The therapy of infection due to Pneumocystis carinii has evolved in two areas over the past 20 years.First, improvements in the diagnosis of Pneumocystis infection have facilitated early diagnosis; treatment can often be initiated before marked deterioration in pulmonary function is observed.Second, the prolonged survival of immunocompromised patients has altered the presentation of Pneumocystis pneumonia, with there being an increased incidence of patients presenting with, in addition to P. carinii, multiple opportunistic infections often with some degree of resistance to common antimicrobial agents; the development of atypical (e.g., extrapulmonary) presentations of disease; and a growing number of patients requiring alternative prophylactic regimens due to drug toxicity (19a).Because Pneumocystis infection can be rapidly progressive and the success of therapy is related to the severity of disease at the time of the initiation of therapy, early therapy is essential.The short-term use of treatment (48 h) will not impair the histopathologic diagnosis of infection if, for example, bronchoscopic or laboratory support services are temporarily unavailable.The risk of opportunistic infection may be reduced by decreasing the level of immune suppression in patients receiving exogenous immune suppression for autoimmune diseases or following organ transplantation or by decreasing the viral load in patients infected with human immunodeficiency virus (HIV).Treatment of Pneumocystis should be successful if a 14-to 21-day course of therapy is tolerated. CLINICAL PRESENTATIONA number of atypical clinical features may alter the choice of therapy for Pneumocystis infection.Multiple infections are often present simultaneously with Pneumocystis infection.Multiple infections are often present simultaneously with Pneumocystis infection.Thus, failure to respond to appropriate anti-Pneumocystis therapy may reflect the progression of another process which may require treatment before clinical improvement is observed.Response to appropriate therapy may be slow.Response to appropriate therapy may be slow, especially in those with severe respiratory compromise or concurrent underlying lung disease.Radiologic appearance lags behind clinical deterioration or improvement.Radiologic appearance lags behind clinical deterioration or improvement, most commonly in the patient with altered pulmonary parenchyma due to radiation therapy, chemotherapy, or prior surgery; or in the patient receiving corticosteroid therapy; or in the patient with neutropenia.Empiric therapy is generally avoided in the immunocompromised host without AIDS.Empiric therapy is generally avoided
Electroactive Polymer-Based Devices for e-Textiles in Biomedicine (2005)

This paper describes the early conception and latest developments of electroactive polymer (EAP)-based sensors, actuators, electronic components, and power sources, implemented as wearable devices for smart electronic textiles (e-textiles). Such textiles, functioning as multifunctional wearable human interfaces, are today considered relevant promoters of progress and useful tools in several biomedical fields, such as biomonitoring, rehabilitation, and telemedicine. After a brief outline on ongoing research and the first products on e-textiles under commercial development, this paper presents the most highly performing EAP-based devices developed by our lab and other research groups for sensing, actuation, electronics, and energy generation/storage, with reference to their already demonstrated or potential applicability to electronic textiles.
Emotional Reactions to the Perception of Risk in the Pompeii Archaeological Park (2020)

The assessment of perceived risk by people is extremely important for safety and security management. Each person is based on the opinion of others to make a choice and the Internet represents the place where these opinions are mostly researched, found and reviewed. Social networks have a decisive impact: 92% of consumers say they have more trust in social media reviews than in any other form of advertising. For this reason, Opinion Mining and Sentiment Analysis have found interesting applications in the most diverse context, among which the most innovative is certainly represented by public safety and security. Security managers can use the perceptions expressed by people to discover the unexpected and potential weaknesses of a controlled environment or otherwise the risk and security perception of people that sometimes can be very different from real level of risk and security of a given site. Since the perceptions are the result of mostly unconscious elaborations, it is necessary to go deeper and to search for the emotions, triggered by the sensorial stimuli, that determine them. The objective of this paper is to study the perception of risk within the Pompeii Archaeological Park, giving emphasis to the emotional components, using the semantic analysis of the textual contents present in Twitter.
Do Physician Practice Styles Persist over Time? Continuities in Patterns of Clinical Decision-Making among General Practitioners (2000)

This study confirms the existence of substantial and durable individual practice styles in primary medical care, with implications for the development and successful implementation of clinical guidelines.
Synthesis of N-<i>t</i>-Boc-4-<i>S</i>-<i>t</i>-Butyl-L-thiophenylalanine<i>via</i>Palladium Catalyzed Cross-Coupling Reaction of N-<i>t</i>-Boc-4-Iodo-L-phenylalanine with<i>t</i>-Butylthiol or Sodium<i>t</i>-Butylthiolate (1996)

Abstract Cross-coupling reaction of N-t-Boc-4-iodo-L-phenylalanine with t-BuSH proceeded efficiently in the presence of Pd2dba3.CHCl3/DPPF as the catalyst system. However, Pd2dba3.CHCl3/DPPB was found to be more effective for coupling with t-BuSNa.
GENDERED PERSPECTIVES ON MEDICINE: AN INTRODUCTION (2005)

This is a volume about gender, health and medicine broadly defined. It is based on the now widely-held assumption in the sociology of medicine that medicine and health are social constructions and that gender is an embedded part of them (see Lorber, 1997). The essays reveal that embedded with gender in the institution of medicine are race, class, and sexuality. Taken as a whole, the volume offers a critique of exclusively biomedical approaches to personal and public health and calls for more sociological input and qualitative research to help us understand aspects of health and illness. Among the recurrent themes in the seven essays are the medicalization of personal and social problems, the commodification of healthcare, and questions of agency, responsibility and control.
Activation of montmorillonite by ion exchange and sorption complexes of tetra-alkyl ammonium montmorillonites (1955)

R. M. Barrer and D. M. MacLeod, Trans. Faraday Soc., 1955, 51, 1290 DOI: 10.1039/TF9555101290
Concentrated Hydrogen Peroxide as a Propellant (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTConcentrated Hydrogen Peroxide as a PropellantNoah S. Davis and John H. KeefeCite this: Ind. Eng. Chem. 1956, 48, 4, 745–748Publication Date (Print):April 1, 1956Publication History Published online1 May 2002Published inissue 1 April 1956https://pubs.acs.org/doi/10.1021/ie50556a025https://doi.org/10.1021/ie50556a025research-articleACS PublicationsRequest reuse permissionsArticle Views519Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Soft Substrate Up-regulates the Interaction of STIM1 with Store-operated Ca<sup>2+</sup>Channels That Lead to Normal Epithelial Cell Apoptosis (2008)

We have demonstrated that soft substrate induced apoptosis in polarized cells, but not in transformed cells by disturbance of Ca(2+) homeostasis. This study aims to further investigate the regulatory mechanisms underlying the disruption of Ca(2+)-signaling integrity in soft substrate-induced epithelial apoptosis. Soft substrate up-regulated the store-operated Ca(2+) (SOC) entry across the plasma membrane of normal cervical epithelial cells, which resulted in increased cytosolic Ca(2+) levels. Concomitantly, soft substrate induced the aggregation and translocation of stromal interacting molecule 1 (STIM1) toward the cell periphery to colocalize with Orai1, an essential pore subunit of SOC channel, detected by fluorescence resonance energy transfer approach and confocal image analyses. The disturbed Ca(2+) homeostasis resulted in the activation of mu-calpain, which cleaved alpha-spectrin, induced actin disorganization, and caused apoptosis. In contrast, soft substrate did not disturb Ca(2+) homeostasis or induce apoptosis in cervical cancer cells. Chelating extracellular Ca(2+) by EGTA and down-regulated SOC entry by small interfering RNA targeting STIM1 or inhibitors targeting Ca(2+)-binding site of calpain significantly inhibited soft substrate-induced activation of mu-calpain and epithelial cell apoptosis. Thus, soft substrate up-regulates the interaction of STIM1 with SOC channels, which results in the activation of mu-calpain and subsequently induces normal epithelial cell apoptosis.
Trends in cancer incidence in Connecticut, 1935–1991 (1994)

Reducing cancer incidence rates will require expanded primary prevention efforts (mainly involving behavioral changes) and more etiologic research on common cancers whose causes are poorly understood (e.g., breast and prostate cancer) and rarer cancers showing unexplained recent increases (i.e., testicular cancer and Hodgkin's disease).