gebhart/patrepeval huggingface dataset:
Lactogenic Hormones: Binding Sites, Mammary Growth, Secretory Cell Differentiation, and Milk Biosynthesis in Ruminants (1985)

Roles of the lactogenic hormones prolactin and placental lactogen in mammary development in ruminants were reviewed. In contrast with other ruminants, failure to detect lactogenic activity in the serum of pregnant cows (in excess of that attributed to prolactin) suggests that placental lactogen may have little direct effect on mammary growth or lactogenesis. However, replacement and ablation experiments using ergocryptine provide definitive evidence that increased periparturient secretion of prolactin is necessary for maximal milk production in cattle. Quantitative microscopy indicates a relative failure of mammary cells in cows with inhibited secretion of prolactin to differentiate structurally. Prolactin induces synthesis and secretion of alpha-lactalbumin in prepartum bovine mammary tissue. Temporary disruption of mammary microtubules immediately prepartum in pregnant heifers reduced subsequent milk production, biosynthetic capacity, and cellular differentiation. For maximal milk production, mammary secretory cells apparently must respond to lactogenic hormone stimulation during the immediate periparturient period. Colchicine may desensitize the mammary epithelium to prolactin action. Membrane binding of radiolabeled human growth hormone to ruminant mammary gland provides a measure of lactogenic hormone binding sites. Specific binding to 600 micrograms of mammary membrane protein was 296% greater in lactating, compared with nonlactating, pregnant (65 days of gestation) ewes. Binding capacity (fmol/mg membrane protein) averaged 275 +/- 57 in mammary membranes from nonlactating, pregnant ewes (100 days gestation, n = 2) and 2,325 +/- 521 in mammary membranes from lactating ewes (n = 6, 14 to 21 days postpartum). Greater understanding of hormonal regulation of the ruminant mammary gland likely will result in development of techniques to produce milk more efficiently and perhaps capability to evaluate production potential of young animals.
Monolithic Isolated Single-Mode Ring Laser (1987)

MISER uses four-sided ring to lase nonreciprocally. Laser concept provides very-compact, efficient, and stable source of coherent radiation. Lasers of single, very-stable frequency desirable for number of purposes. Frequency stability makes possible coherent detection of laser radiation with low-bandwidth detector, useful for communications. Allows very-high-resolution spectroscopy. Any application where desired to use laser in way microwaves used traditionally enhanced if frequency very stable.
Newly identified proteins in human nasal lavage fluid from non-smokers and smokers using two-dimensional gel electrophoresis and peptide mass fingerprinting (2002)

Human nasal lavage fluids (NLFs) were analyzed with two-dimensional gel electrophoresis (2-DE) and proteins were identified with peptide mass fingerprinting using matrix-assisted laser desoption/ionization time of flight mass spectrometry. In some cases, the identification was verified by analysis of post-source decay fragmentation spectra. Many of the identified proteins were new forms or fragments of previously found proteins (e.g. albumin, lactoferrin, cystatin, calgranulin, von Ebners gland protein and palate lung nasal epithelium clone), while others were proteins that have previously been indicated by 2-DE image matching or immunoblots (e.g. apolipoprotein AI, lysozyme C, and Clara cell secretory protein). Some new proteins, not shown before in 2-DE patterns of NLF were also found, e.g. mammaglobin B, (2)-microglobulin and immunoglobulin J chain. Of the identified NLF proteins many appear to be involved in inflammatory and immune responses. A study was therefore conducted to investigate if the levels of these proteins were changed in smokers compared to nonsmokers. It was found that NLF from smokers contained decreased levels of Clara cell secretory protein, and increased proportions of a truncated variant of lipocortin-1, three acidic forms of alpha(1)-antitrypsin, and one phosphorylated form of cystatin S. Furthermore, NLF from smokers contained increased proportions of a new variant of palate lung nasal epithelium clone (PLUNC), a recently identified airway irritation marker. The results demonstrate that 2-DE of NLF may be used to assess alterations of proteins or post-translationally modified proteins in smokers. Clara cell secretory protein (CC 16, CC 10) and lipocortin-1 are two anti-inflammatory, phospholipase A(2) inhibitory proteins, and alpha(1)-antitrypsin and cystatin S are two proteinase inhibitors. Changed levels of these proteins may therefore be of importance to the airway inflammation caused by smoking. The results also support the notion that PLUNC is involved in inflammatory responses in the upper airways.
Evaluation of multiwavelength first- and second-derivative spectra for the quantitation of mixtures of polynuclear aromatic hydrocarbons (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEvaluation of multiwavelength first- and second-derivative spectra for the quantitation of mixtures of polynuclear aromatic hydrocarbonsYahya R. Tahboub and Harry L. PardueCite this: Anal. Chem. 1985, 57, 1, 38–41Publication Date (Print):January 1, 1985Publication History Published online1 May 2002Published inissue 1 January 1985https://pubs.acs.org/doi/10.1021/ac00279a014https://doi.org/10.1021/ac00279a014research-articleACS PublicationsRequest reuse permissionsArticle Views128Altmetric-Citations37LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optimal methods to characterize the G93A mouse model of ALS (2005)

In the present study, we used the SOD1 (G93A) mutant transgenic mice as a model of amyotrophic lateral sclerosis (ALS). This model is widely used as a laboratory tool to study experimental treatments in vivo for ALS to investigate new therapeutic strategies for this neurodegenerative disease. Such studies require the objective quantification of different parameters while mice develop the disease. We have applied a battery of different and specific tests: scoring of motor deficits by a trained observer, weighing, survival measure, hanging wire test, rotarod task and electromyography, most of them commonly used to evaluate G93A animals. We have critically compared these methods, showing the significant influence of gender on the onset of symptoms, and the optimal moment to apply each test. These results should be taken into account in future therapeutic assays on this ALS model.
Integrated laser-modulator-amplifier in an identicallayer with two different multiquantum wells (1998)

A modulator with low chirp parameter (< 0.5) at moderate bias voltage (~ 0 V) was integrated with a semiconductor laser and amplifier. The indentical active layer contains two different multiquantum wells (MQW) in the transverse direction in all three parts. These two different MQWs improve the chirp behaviour of the modulator and the amplification.
Anomalous Raman scattering from the surface of conjugated polymer melts (2001)

Confocal Raman spectroscopy from the surface of a polymer melt is shown to produce anomalous results for polarized Raman scattering of specific molecular vibrations. Values of the depolarization ratio that cannot exist in normal liquids are interpreted as due to surface-induced molecular orientation of the polymer chains. The technique provides one of the few direct experimental evidences of molecular-orientation effects predicted in lattice models of polymer melts.
Photonic Hydrogels with Poly(ethylene glycol) Derivative Colloidal Spheres as Building Blocks (2008)

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication to the EditorNEXTPhotonic Hydrogels with Poly(ethylene glycol) Derivative Colloidal Spheres as Building BlocksTong Cai†, Guonan Wang†, Samuel Thompson†, Manuel Marquez‡§⊥, and Zhibing Hu*†View Author Information Department of Physics, University of North Texas, P.O. Box 311427, Denton, Texas 76203; Harrington Department of Bioengineering, Arizona State University, Tempe, Arizona 85287; NIST Center for Theoretical and Computational Nanosciences, Gaithersburg, Maryland 20899; and Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545* To whom correspondence should be addressed. E-mail: [email protected]†University of North Texas.‡Arizona State University.§NIST Center for Theoretical and Computational Nanosciences.⊥Los Alamos National Laboratory.Cite this: Macromolecules 2008, 41, 24, 9508–9512Publication Date (Web):November 19, 2008Publication History Received5 September 2008Revised12 November 2008Published online19 November 2008Published inissue 23 December 2008https://pubs.acs.org/doi/10.1021/ma802035dhttps://doi.org/10.1021/ma802035drapid-communicationACS PublicationsCopyright © 2008 American Chemical SocietyRequest reuse permissionsArticle Views2416Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Gels,Hydrogels,Polymer particles,Reaction products,Thin films Get e-Alerts
Morbidity, Mortality, and Healthcare Burden of Nosocomial <i>Clostridium Difficile</i> -Associated Diarrhea in Canadian Hospitals (2002)

N-CDAD is a common and serious nosocomial infectious complication in Canada, is associated with substantial morbidity and mortality, and imposes an important financial burden on healthcare institutions.
Corroborating tomographic defect metrics with mechanical response in an additively manufactured precipitation-hardened stainless steel (2018)

The intrinsic relation between structure and performance is a foundational tenant of most all materials science investigations. While the specific form of this relation is dictated by material system, processing route and performance metric of interest, it is widely agreed that appropriate characterization of a material allows for greater accuracy in understanding and/or predicting material response. However, in the context of additive manufacturing, prior models and expectations of material performance must be revisited as performance often diverges from traditional values, even among well explored material systems. This work utilizes micro-computed tomography to quantify porosity and lack of fusion defects in an additively manufactured stainless steel and relates these metrics to performance across a statistically significant population using high-throughput mechanical testing. The degree to which performance in additively manufactured stainless steel can and cannot be correlated to detectable porosity will be presented and suggestions for performing similar experiments will be provided.
Producers strive to bring kainic acid back on the market (2000)

RETURN TO ISSUEPREVNewsNEXTProducers strive to bring kainic acid back on the marketJEAN-FRANÇOIS TREMBLAYCite this: Chem. Eng. News 2000, 78, 10, 31Publication Date (Print):March 6, 2000Publication History Published online12 November 2010Published inissue 6 March 2000https://pubs.acs.org/doi/10.1021/cen-v078n010.p031https://doi.org/10.1021/cen-v078n010.p031newsACS PublicationsCopyright © 2000 American Chemical SocietyArticle Views17Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Central nervous system,Pharmaceuticals,Seawater Get e-Alerts
Preoperative diagnosis of Meckel's diverticulum in a 62-year-old man: utility of an angiographic technique. (1993)

A 62-year-old man with severe anal bleeding was admitted to our ward as an emergency patient. Angiography of the superior mesenteric artery revealed an omphalomesenteric artery. Methylene blue was injected superselectively into the artery through an angiographic catheter at the time of subsequent laparotomy. Blood supply through the artery to the restricted area of the diverticulum could thus be defined in situ.
Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses (2011)

Adeno-associated virus (AAV)-based vectors expressing therapeutic gene products have shown great promise for human gene therapy. A major challenge for translation of promising research to clinical development is the establishment of appropriate quality control (QC) test methods to characterize clinical grade AAV vectors. This chapter focuses on QC testing, providing an overview of characterization methods appropriate for clinical vectors prepared for early phase clinical studies, and detailed descriptions for selected assays that are useful to assess AAV vector safety, potency, and purity.
Accumulation of Streptococcus mutans on Light‐Cured Composites and Amalgam: An In Vitro Study (1998)

The aim of the in vitro study was to examine the accumulation of Streptococcus mutans on light-cured composite materials and amalgam. Bacteria cultures were grown in a brain heart infusion medium, and their growth rate was determined through turbidity measurements. The data, so obtained, were evaluated statistically by analysis of variance (ANOVA) followed by Scheffe test. Experiments on amalgam showed better results compared to those on composite materials. There were no statistically significant differences in plaque accumulation on different composite materials after finishing and polishing procedures, compared to plaque accumulation on composite materials against a Mylar strip.
Purification, Characterization, and Sequence Determination of Phospholipase D Secreted by Streptoverticillium cinnamoneum (1999)

Phospholipase D (PLD), secreted into the culture medium of an actinomycete, Streptoverticillium cinnamoneum, has been purified to homogeneity and characterized. The Stv. cinnamoneum PLD efficiently catalyzes both the hydrolysis and transphosphatidylation of various phospholipids, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), and phosphatidylserine (PS). However, the substrate specificity differs between the two reactions; PE serves as the most preferred substrate for the hydrolysis, but PC and PS are better substrates than PE for the transphosphatidylation. In addition, the transphosphatidylation but not the hydrolysis of PE and PC is markedly activated on the addition of metal ions, especially Al3+. Nucleotide and amino acid sequence determination of the Stv. cinnamoneum PLD revealed the presence of common structural motifs identified in all PLD sequences from various species.
Properties of .alpha.-keto cations. Facile generation under solvolytic conditions (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProperties of .alpha.-keto cations. Facile generation under solvolytic conditionsXavier Creary and Cristina C. GeigerCite this: J. Am. Chem. Soc. 1982, 104, 15, 4151–4162Publication Date (Print):July 1, 1982Publication History Published online1 May 2002Published inissue 1 July 1982https://pubs.acs.org/doi/10.1021/ja00379a018https://doi.org/10.1021/ja00379a018research-articleACS PublicationsRequest reuse permissionsArticle Views1354Altmetric-Citations70LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Probiotic<i>L. reuteri</i>Treatment Prevents Bone Loss in a Menopausal Ovariectomized Mouse Model (2014)

Estrogen deficiency is a major risk factor for osteoporosis that is associated with bone inflammation and resorption. Half of women over the age of 50 will experience an osteoporosis related fracture in their lifetime, thus novel therapies are needed to combat post-menopausal bone loss. Recent studies suggest an important role for gut-bone signaling pathways and the microbiota in regulating bone health. Given that the bacterium Lactobacillus reuteri ATCC PTA 6475 (L. reuteri) secretes beneficial immunomodulatory factors, we examined if this candidate probiotic could reduce bone loss associated with estrogen deficiency in an ovariectomized (Ovx) mouse menopausal model. Strikingly, L. reuteri treatment significantly protected Ovx mice from bone loss. Osteoclast bone resorption markers and activators (Trap5 and RANKL) as well as osteoclastogenesis are significantly decreased in L. reuteri-treated mice. Consistent with this, L. reuteri suppressed Ovx-induced increases in bone marrow CD4+ T-lymphocytes (which promote osteoclastogenesis) and directly suppressed osteoclastogenesis in vitro. We also identified that L. reuteri treatment modifies microbial communities in the Ovx mouse gut. Together, our studies demonstrate that L. reuteri treatment suppresses bone resorption and loss associated with estrogen deficiency. Thus, L. reuteri treatment may be a straightforward and cost-effective approach to reduce post-menopausal bone loss.
SYBR Green-Based Real-Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False-Negative Results Due to Strain Variability (2004)

Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus (WNV). Rapidly evolving RNA viruses present a challenge for diagnosis because they accumulate mutations that may render them undetectable. To explore the effect of sequence variations on assay performance, we generated every possible single point mutation within the target region of the widely used TaqMan assay for WNV and found that the TaqMan assay failed to detect 47% of possible single nucleotide variations in the probe-binding site and was unable to detect any targets with more than two mutations. In response, we developed and validated a less expensive assay with the intercalating dye SYBR green. The SYBR green-based assay was as sensitive as the TaqMan assay for WNV. Importantly, it detected 100% of possible WNV target region variants. The assay developed here adds an additional layer of protection to guard against false-negative results that result from natural variations or drug-directed selection and provides a rapid means to identify such variants for subsequent detailed analysis.
Characterization of a cytokinesis defective (cyd1) mutant of Arabidopsis (1999)

Although several mutations and genes affecting plant cytokinesis have been identified, mutant screens are not yet saturated and knowledge about gene function is still limited. A novel Arabidopsis mutation, cytokinesis defective1 (cyd1), was identified by partial or missing cell walls in stomata. Stomata with incomplete or no cytokinesis still differentiate and some contain swellings of the outer wall not found in the wild type. The incomplete walls are correctly placed opposite stomatal wall thickenings suggesting that the mutation interferes with the execution of cytokinesis rather than with the placement of the division site. Cytokinesis defects are also detectable in other cell types throughout the plant, defects which include cell wall protrusions, two or more nuclei in one cell, and reduced cell number. The extent of cytokinetic partitioning correlates with nuclear number in abnormal stomata. Many cyd1 epidermal cells, stomata and pollen are larger, and trichomes have more branches. cyd1 is partially lethal with poor seed set and some defective ovules, but many plants are fertile despite abnormalities in vegetative and reproductive development such as missing, reduced, fused or misshapen leaves and floral organs. cyd1 appears to be the only cytokinesis mutant described where defects are known to occur in both mature vegetative and reproductive organs. Thus, the CYD1 gene product appears to be necessary for the execution of cytokinesis throughout the shoot. The examination of stomata by microscopy may be a useful screen for the directed isolation of additional cytokinesis mutations that are not embryo or seedling lethal
Phosphorous Acid and Urea: Valuable Sources of Phosphorus and Nitrogen in the Hydrothermal Synthesis of Ammonium-Thorium Phosphates (2008)

Microcrystals of the first ammonium-thorium phosphates, (NH 4) 2Th(PO 4) 2.H 2O (tetragonal, I4 1/ amd, a = 7.0192(4) A, c = 17.9403(8) A) and NH 4Th 2(PO 4) 3 (monoclinic, C2/ c, a = 17.880(6) A, b = 6.906(1) A, c = 8.152(2) A, beta = 104.39(2) degrees ) were hydrothermally obtained from a Th(NO 3) 4-CO(NH 2) 2-H 3PO 3-H 2O system ( T = 180 degrees C). In both cases, the structure consists of a three-dimensional framework with PO 4 tetrahedra coordinated to Th atoms (ThO n polyhedra, where n = 8 or 9, for the tetragonal or monoclinic phase, respectively). The ammonium ions (and water molecules) are located in the tunnels.