gebhart/patrepeval huggingface dataset:
α7 Nicotinic Acetylcholine Receptors on GABAergic Interneurons Evoke Dendritic and Somatic Inhibition of Hippocampal Neurons (2002)

GABAergic interneurons in the hippocampus express high levels of alpha7 nicotinic acetylcholine receptors, but because of the diverse roles played by hippocampal interneurons, the impact of activation of these receptors on hippocampal output neurons (i.e., CA1 pyramidal cells) is unclear. Activation of hippocampal interneurons could directly inhibit pyramidal neuron activity but could also produce inhibition of other GABAergic cells leading to disinhibition of pyramidal cells. To characterize the inhibitory circuits activated by these receptors, exogenous acetylcholine was applied directly to CA1 interneurons in hippocampal slices, and the resulting postsynaptic responses were recorded from pyramidal neurons or interneurons. Inhibitory currents mediated by GABA(A) receptors were observed in 27/131 interneuron/pyramidal cell pairs, but no instances of disinhibition of spontaneous inhibitory events or GABA(B) receptor-mediated responses were observed. Two populations of bicuculline-sensitive GABA(A) receptor-mediated currents could be distinguished based on their kinetics and amplitude. Anatomical reconstructions of the interneurons in a subset of connected pairs support the hypothesis that these two populations correspond to inhibitory synapses located either on the somata or dendrites of pyramidal cells. In 11 interneuron/interneuron cell pairs, one presynaptic neuron was observed that produced strong inhibitory currents in several nearby interneurons, suggesting that disinhibition of pyramidal neurons may also occur. All three types of inhibitory responses (somatic-pyramidal, dendritic-pyramidal, and interneuronal) were blocked by the alpha7 receptor-selective antagonist methyllycaconitine. These data suggest activation of these functionally distinct circuits by alpha7 receptors results in significant inhibition of both hippocampal pyramidal neurons as well as interneurons.
Synthesis of Titanium CCC-NHC Pincer Complexes and Catalytic Hydroamination of Unactivated Alkenes (2012)

The previously reported amine elimination methodology using group 4 tetrakis(dimethylamido) starting materials for the synthesis of CCC-NHC pincer Zr and Hf complexes has been extended to include Ti. Use of excess Ti(NMe2)4 with 1,3-bis(3-butylimidazol-1-yl)benzene diiodide (1) yielded (1,3-bis(3-butylimidazol-1-yl-2-idene)-2-phenylene)bis(dimethylamido)iodotitanium(IV) (2), which subsequently reacted with CH2Cl2 to afford (1,3-bis(3-butylimidazol-1-yl-2-idene)-2-phenylene)trichlorotitanium(IV) (3). Combining complex 2 with TMSCl yielded complex 3 along with the geometric isomers of (1,3-bis(3-butylimidazol-1-yl-2-idene)-2-phenylene)dichloroiodotitanium(IV) (4a,e). The salt, 1,3-bis(3-butylimidazol-1-yl)benzene dichloride (5), was reacted with Ti(NMe2)4 in a stoichiometric amount to yield (1,3-bis(3-butylimidazol-1-yl-2-idene)-2-phenylene)dichloro(dimethylamido)titanium(IV) (6), while use of excess Ti(NMe2)4 yielded (1,3-bis(3-butylimidazol-1-yl-2-idene)-2-phenylene)chlorobis(dimethylamido)titanium(IV) (7). An efficient direct synthesis of trichloro complex 3 was accomplished by reacting the chlorobis(dimethylamido)titanium complex 7 with TMSCl. The catalytic activity of the bis(dimethylamido)iodotitanium complex 2 and chlorobis(dimethylamido)titanium complex 7 toward the hydroamination/cyclization of unactivated alkene-amines was also evaluated.
Autologous Cultured Chondrocytes for the Treatment of Knee Cartilage Injury (2000)

Injury to articular cartilage is a problem causing debilitating symptoms that often require surgical intervention. Traditional surgeries provide treatments to relieve symptoms of pain, locking, and swelling. With advances in technology, chondral defects can now be repaired. By repairing the cartilage, individuals have a far better chance of having healthy, active lifestyles including high activity, such as sports, or normal activities of daily living, such as working. But everyone is not a candidate for cartilage repair. By understanding the significance of the injury and the available surgical interventions, nurses can provide information that can enable a favorable outcome.
A review on synthesis of value added products from polyethylene terephthalate (PET) waste (2013)

Many research papers have been contributed by several authors for making PET waste recycling economically and ecologically more viable. Recycling of PET waste was started in last two decades. Most of the authors are devoting their time in getting economically viable solution for development of methods based on either mechanical or chemical recycling. Some success has been obtained in development of chemical recycling methods which provides value added products from PET waste. However, different products developed by chemical recycling have not provided economically enough and reliable methods of recycling of PET waste.
Automatic detection and segmentation of microcalcifications on digitized mammograms (1992)

We have studied the use of computer-based methods to the detection and segmentation of microcalcifications in digital mammograms. The computer detection system is based on the rhmaxima algorithm applied twice to two filtered images, where we have automated the parameters r and h. The segmentation is based on an algorithm derived from the watershed transform by using different markers for the background and microcalcifications. The potential application of such a computer-aided system to mammographic interpretation is demonstrated by its ability to detect microcalcifications in clinical mammograms.
Circulating cell-free DNA in serum as a biomarker of colorectal cancer (2013)

Aims The aim of this study was to report circulating cell-free DNA using ALU247 and ALU247/ALU115 biomarkers in serum of operated and non-operated patients with colorectal cancer (CRC). Methods To undertake this, 90 blood samples were collected, including 30 samples from healthy volunteers; 27 samples from CRC non-operated patients and 33 samples from CRC-operated patients. Circulating cell-free DNA was verified through quantitative real-time PCR (qPCR) using ALU115 and ALU247 primers. Results With regard to the ALU115-qPCR biomarker, the increased levels of circulating cell-free DNA in serum of non-operated patients were significant when compared with control (p<0.05). Moreover, levels of ALU247-qPCR biomarker were statistically significant between non-operated versus operated and non-operated versus control groups (p=0.000). With regard to the ALU247/115-qPCR biomarker, significant differences were observed between control versus non-operated patients (p=0.019), operated versus non-operated patients (p=0.005) and control versus operated patients (p=0.043). Conclusions Thus, the ALU247 and ALU247/ALU115-qPCR biomarkers may be important in detecting and monitoring CRC patients in both early and late stages.
Enhanced Photosynthesis and Redox Energy Production Contribute to Salinity Tolerance in Dunaliella as Revealed by Homology-Based Proteomics (2004)

Salinity is a major limiting factor for the proliferation of plants and inhibits central metabolic activities such as photosynthesis. The halotolerant green alga Dunaliella can adapt to hypersaline environments and is considered a model photosynthetic organism for salinity tolerance. To clarify the molecular basis for salinity tolerance, a proteomic approach has been applied for identification of salt-induced proteins in Dunaliella. Seventy-six salt-induced proteins were selected from two-dimensional gel separations of different subcellular fractions and analyzed by mass spectrometry (MS). Application of nanoelectrospray mass spectrometry, combined with sequence-similarity database-searching algorithms, MS BLAST and MultiTag, enabled identification of 80% of the salt-induced proteins. Salinity stress up-regulated key enzymes in the Calvin cycle, starch mobilization, and redox energy production; regulatory factors in protein biosynthesis and degradation; and a homolog of a bacterial Na(+)-redox transporters. The results indicate that Dunaliella responds to high salinity by enhancement of photosynthetic CO(2) assimilation and by diversion of carbon and energy resources for synthesis of glycerol, the osmotic element in Dunaliella. The ability of Dunaliella to enhance photosynthetic activity at high salinity is remarkable because, in most plants and cyanobacteria, salt stress inhibits photosynthesis. The results demonstrated the power of MS BLAST searches for the identification of proteins in organisms whose genomes are not known and paved the way for dissecting molecular mechanisms of salinity tolerance in algae and higher plants.
Analysis of depletion edge translation lightwave modulators (1988)

Presents a complete analysis of waveguide phase modulators based on the depletion-edge-translation concept. The phenomena taking place inside the depletion region which contribute to changing the refractive index there are studied. It is shown that the behavior of these modulators can be understood in terms of two electric field-related and two carrier-related effects: linear electrooptic, electrorefractive, plasma, and band filling. The sum of the refractive index variations produced by each one of these effects, taking into account the waveguide geometry, accounts quantitatively for the experimental phase shifts measured in the devices. No fitting parameters are used and a very good agreement between theory and experiment is obtained. Based on this theory, an analysis of the device is made in terms of the optimum values for the doping in the waveguide, and also in terms of the wavelength dependence of the device phase modulation properties.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Bandwidth estimation for ultra-high-speed lithium niobate modulators (2003)

The effects of velocity matching, impedance matching, conductor loss, and dielectric loss on the optical bandwidth of an ultra-high-speed lithium niobate modulator are reported. It is shown that both dielectric loss and impedance matching play a key role for velocity-matched high-speed modulators with low conductor loss. The effects of etch depth, buffer thickness, electrode width, and thegap between the electrodes on device performance are also illustrated.
Binding of <sup>125</sup>I‐labeled granulocyte colony‐stimulating factor to normal murine hemopoietic cells (1985)

The binding of granulocyte colony-stimulating factor (G-CSF) to murine bone marrow cells was investigated using a radioiodinated derivative of high specific radioactivity which retained full biological activity. The binding was time- and temperature-dependent, saturable and highly specific. The apparent dissociation constant for the reaction was 60-80 pM at 37 degrees C and 90-110 pM at 4 degrees C, similar to that found for the binding of G-CSF to murine leukemic cells (WEHI-3B D+) and significantly higher than the concentration of G-CSF required to stimulate colony formation in vitro. Autoradiographic analysis confirmed the specificity of binding since granulocytic cells were labeled but lymphocytes, erythroid cells and eosinophils were not. Blast cells and monocytic cells were partially labeled, the latter at low levels. In the neutrophilic granulocyte series, grain counts increased with cell maturity, polymorphs being the most heavily labeled but all cells showed considerable heterogeneity in the degree of labeling. Combination of Scatchard analysis of binding with autoradiographic data indicated that mature granulocytes from murine bone marrow exhibited 50-500 G-CSF receptors per cell.
To brake or not to brake? Is there a question? (2002)

The vehicle following problem in an automated highway is approached from the point of view of safety. In particular the situation where an intervehicle collision is unavoidable and the relative velocity at impact is what determines the safety of the situation, is considered. It is shown, using tools from optimal control, that braking as hard as possible is not the best possible strategy in all cases. More importantly, the assumption that the worst that the vehicle ahead can do is to brake as hard as possible may also be incorrect.
Optimum symbol-by-symbol detection of uncoded digital data over the Gaussian channel with unknown carrier phase (1994)

A theory of optimum receiver design for symbol-by-symbol detection of an uncoded digital data sequence received over the Gaussian channel with unknown carrier phase is presented. Linear suppressed-carrier modulation is assumed. The work here aims at laying a conceptual foundation for optimum symbol-by-symbol detection, and rectifies existing approaches to the problem. The optimum receiver structure is obtained explicitly for an arbitrary carrier phase model, but its computational requirements are too heavy in general for any practical implementation. In one important special case, namely, the case in which the carrier phase can be treated as a constant over some K+1 symbol intervals, the optimum receiver can be approximated by a readily implementable decision-feedback structure at high SNR. Simulated error performance results are presented for this latter receiver for PSK modulations, with various carrier phase models. Since a decision-feedback receiver can encounter a "runaway", a variation of this receiver is developed which uses feedforward of tentative decisions concerning future symbols. This modified receiver does not have any "runaway" problem, and has been shown to yield good error performance via simulations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A VLSI design for full search block matching motion estimation (2002)

In this paper, we describe a flexible VLSI architecture to achieve a real-time processing of full-search block matching algorithm (FBMA) for video applications. The proposed architecture uses a parallel algorithm based on the idea of partial result accumulation. The partial sum results of the candidate block distortions are individually accumulated into cyclic storage buffer for each distortion measure. A parameterizable Motion Estimation Processor (MEP) is designed for both different reference block sizes and various search ranges. Moreover, for larger search ranges and high throughput rates, multiple number of MEPs can be cascaded. It has serial data input but performs parallel processing. It can be easily and cost-effectively implemented into VLSI by its simple one dimensional semi-systolic array architecture and control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Regulation of Inflammatory Response in Neural Cells in Vitro by Thiadiazolidinones Derivatives through Peroxisome Proliferator-activated Receptor γ Activation (2005)

In most neurodegenerative disorders, including multiple sclerosis, Parkinson disease, and Alzheimer disease, a massive neuronal cell death occurs as a consequence of an uncontrolled inflammatory response, where activated astrocytes and microglia and their cytotoxic agents play a crucial pathological role. Current treatments for these diseases are not effective. In the present study we investigate the effect of thiadiazolidinone derivatives, which have been recently suggested to play a role in neurodegenerative disorders. We have found that thiadiazolidinones are potent neuroprotector compounds. Thiadiazolidinones inhibited inflammatory activation of cultured brain astrocytes and microglia by diminishing lipopolysaccharide-induced interleukin 6, tumor necrosis factor alpha, inducible nitric-oxide synthase, and inducible cyclooxygenase type 2 expression. In addition, thiadiazolidinones inhibited tumor necrosis factor-alpha and nitric oxide production and, concomitantly, protected cortical neurons from cell death induced by the cell-free supernatant from activated microglia. The neuroprotective effects of thiadiazolidinones are completely inhibited by the peroxisome proliferator-activated receptor gamma antagonist GW9662. In contrast the glycogen synthase kinase 3beta inhibitor LiCl did not show any effect. These findings suggest that thiadiazolidinones potently attenuate lipopolysaccharide-induced neuroinflammation and reduces neuronal death by a mechanism dependent of peroxisome proliferator-activated receptor gamma activation.
Stromal galectin-1 expression is associated with long-term survival in resectable pancreatic ductal adenocarcinoma (2012)

The overall 5 year survival rate for pancreatic ductal adenocarcinoma (i.e., PDAC) is a dismal 5%, although patients that have undergone surgical resection have a somewhat better survival rate of up to 20%. Very long-term survivors of PDAC (defined as patients with ≥ 10 year survival following apparently curative resection), on the other hand, are considerably less frequent. The molecular characteristics of very long-term survivors (VLTS) are poorly understood, but might provide novel insights into prognostication for this disease. In this study, a panel of five VLTS and stage-matched short-term survivors (STS, defined as disease-specific mortality within 14 months of resection) were identified, and quantitative proteomics was applied to comparatively profile tumor tissues from both cohorts. Differentially expressed proteins were identified in cancers from VLTS vs. STS patients. Specifically, the expression of galectin-1 was 2-fold lower in VLTS compared with STS tumors. Validation studies were performed by immunohistochemistry (IHC) in two additional cohorts of resected PDAC, including: 1) an independent cohort of VLTS and 2) a panel of sporadic PDAC with a considerable range of overall survival following surgery. Immunolabeling analysis confirmed that significantly lower expression of stromal galectin-1 was associated with VLTS (p = 0.02) and also correlated with longer survival in sporadic, surgically-treated PDAC cases (hazard ratio = 4.9, p = 0.002). The results from this study provide new insights to better understand the role of galectin-1 in PDAC survival, and might be useful for rendering prognostic information, and developing more effective therapeutic strategies aimed at improving survival.
Role of Net Charge on Catalytic Domain and Influence of Cell Wall Binding Domain on Bactericidal Activity, Specificity, and Host Range of Phage Lysins (2011)

The recombinant lysins of lytic phages, when applied externally to Gram-positive bacteria, can be efficient bactericidal agents, typically retaining high specificity. Their development as novel antibacterial agents offers many potential advantages over conventional antibiotics. Protein engineering could exploit this potential further by generating novel lysins fit for distinct target populations and environments. However, access to the peptidoglycan layer is controlled by a variety of secondary cell wall polymers, chemical modifications, and (in some cases) S-layers and capsules. Classical lysins require a cell wall-binding domain (CBD) that targets the catalytic domain to the peptidoglycan layer via binding to a secondary cell wall polymer component. The cell walls of Gram-positive bacteria generally have a negative charge, and we noticed a correlation between (positive) charge on the catalytic domain and bacteriolytic activity in the absence of the CBD (nonclassical behavior). We investigated a physical basis for this correlation by comparing the structures and activities of pairs of lysins where the lytic activity of one of each pair was CBD-independent. We found that by engineering a reversal of sign of the net charge of the catalytic domain, we could either eliminate or create CBD dependence. We also provide evidence that the S-layer of Bacillus anthracis acts as a molecular sieve that is chiefly size-dependent, favoring catalytic domains over full-length lysins. Our work suggests a number of facile approaches for fine-tuning lysin activity, either to enhance or reduce specificity/host range and/or bactericidal potential, as required.
Hybrid transcription system for controlled plastid transgene expression (2006)

Plastid transformation technologies have developed rapidly over the last few years, reflecting their value in the study of the principal mechanisms of plastid gene expression and commercial interest in using plastids as bioreactors. Application of this technology is still limited by the difficulty of obtaining regulated, selective expression of plastid transgenes. The plastid genome is transcribed by two different types of RNA polymerase. One of them is of the eubacterial type of polymerase, and its subunits are encoded in the plastid genome [plastid-encoded RNA polymerase (PEP)]. The other one is of the phage type and nucleus-encoded [nucleus-encoded RNA polymerase (NEP)]. To obtain selective transgene expression, we have made use of the similarities and differences between the eubacterial and the plastid eubacterial type transcription systems. We created a hybrid transcription system in which the transgene is placed under the control of a eubacterial promoter which does not exist in the plastid genome and which is not recognized by the plastid endogenous transcriptional machinery. Selective transcription of the transgene is achieved by the supply of a chimeric transcription factor that interacts with PEP and directs it specifically to the foreign eubacterial-type transgene promoter. This hybrid transcription system could be used for biotechnological and fundamental research applications as well as in the characterization of the evolutionary differences between the eubacterial and the plastid eubacterial-type transcription systems.
Observation of Wakefield Suppression in a Photonic-Band-Gap Accelerator Structure (2016)

We report experimental observation of higher order mode (HOM) wakefield suppression in a room-temperature traveling-wave photonic-band-gap (PBG) accelerating structure at 11.700 GHz. It has been long recognized that PBG structures have the potential for reducing long-range wakefields in accelerators. The first ever demonstration of acceleration in a room-temperature PBG structure was conducted in 2005. Since then, the importance of PBG accelerator research has been recognized by many institutions. However, the full experimental characterization of the wakefield spectrum and demonstration of wakefield suppression when the accelerating structure is excited by an electron beam has not been performed to date. We conducted an experiment at the Argonne Wakefield Accelerator test facility and observed wakefields excited by a single high charge electron bunch when it passes through a PBG accelerator structure. Excellent HOM suppression properties of the PBG accelerator were demonstrated in the beam test.
Comments on "Area-time optimal adder design" (1994)

A previous paper by Wei and Thompson (1990) defined a family of adders based on a modular design and presented an excellent systematic method of implementing a VLSI parallel adder using three types of component cells designed in static CMOS. Their approach to the adder design was based on the optimization of a formulated dynamic programming problem with respect to area and time. The authors first explicitly demonstrate the optimal 32-bit fast carry generator, described by Wei and Thompson, is incorrect. With suitable corrections, a correct 32-bit fast carry generator design is then presented. Next, BiCMOS technology is applied to implement the subcircuit of fast carry generator to accelerate the critical path. The authors show that the critical path delay of 16-bit, 32-bit and 66-bit adders is respectively shortened to 83.89%, 86.89% and 90.62% after introducing the BiCMOS drivers.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>