gebhart/patrepeval huggingface dataset:
Expression of plasmid-encoded aad in Clostridium acetobutylicum M5 restores vigorous butanol production (1994)

Mutant M5 of Clostridium acetobutylicum ATCC 824, which produces neither butanol nor acetone and is deficient in butyraldehyde dehydrogenase (BYDH), acetoacetate decarboxylase, and acetoacetyl-coenzyme A:acetate/butyrate:coenzyme A-transferase activities, was transformed with plasmid pCAAD, which carries the gene aad (R. V. Nair, G. N. Bennett, and E. T. Papoutsakis, J. Bacteriol, 176:871-885, 1994). In batch fermentation studies, aad expression restored butanol formation (84 mM) in mutant M5 without any acetone formation or any significant increase in ethanol production. The corresponding protein (AAD) appeared as a ca. 96-kDa band in a denaturing protein gel. Expression of AAD in M5 resulted in restoration of BYDH activity and small increases in the activities of acetaldehyde dehydrogenase, butanol dehydrogenase, and ethanol dehydrogenase. These findings suggest that BYDH activity in C. acetobutylicum ATCC 824 resides largely in AAD, and that AAD's primary role is in the formation of butanol rather than of ethanol.
Heat Pipes and Heat Rejection Component Testing at NASA Glenn Research Center (2012)

Titanium-water heat pipes are being evaluated for use in the heat rejection system for space fission power systems. The heat rejection syst em currently comprises heat pipes with a graphite saddle and a composite fin. The heat input is a pumped water loop from the cooling of the power conversion system. The National Aeronautics and Space Administration has been life testing titanium-water heat pipes as well as eval uating several heat pipe radiator designs. The testing includes thermal modeling and verification of model, material compatibility, frozen startup of heat pipe radiators, and simulating low-gravity environments. Future thermal testing of titanium-water heat pipes includes low-g ravity testing of thermosyphons, radiation testing of heat pipes and fin materials, water pump performance testing, as well as Small Busine ss Innovation Research funded deliverable prototype radiator panels.
100-kHz-rate gas-phase thermometry using 100-ps pulses from a burst-mode laser (2015)

Temperature measurements based on gas-phase coherent anti-Stokes Raman scattering (CARS) spectroscopy are demonstrated in reacting flows at a rate of 100 kHz employing a burst-mode laser with a pulse duration of ∼100 ps. The recently developed picosecond-duration, high-energy burst-mode laser is used to pump an optical parametric generator/optical parametric amplifier that produces broadband light centered at ∼680 nm to provide the Stokes beams for excitation of the rovibrational Raman transitions of H(2). The 532-nm output of the picosecond burst-mode laser is then utilized as a pump beam for the CARS process that generates 100 single-shot spectra at a rate of 100 kHz during the 1-ms duration burst. Coherent spectroscopy-based temperature measurements at 100 kHz will significantly aid the understanding of transient and unsteady flow phenomena related to turbulent combustion, transonic and hypersonic flows, high-enthalpy flows, and the dynamics of energetic materials.
Properties of an insoluble form of trypsin (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProperties of an insoluble form of trypsinJames D. Ogle and Cora K. GlassmeyerCite this: Biochemistry 1971, 10, 5, 786–792Publication Date (Print):March 1, 1971Publication History Published online1 May 2002Published inissue 1 March 1971https://pubs.acs.org/doi/10.1021/bi00781a010https://doi.org/10.1021/bi00781a010research-articleACS PublicationsRequest reuse permissionsArticle Views119Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Diarylamino Groups as Photostable Auxofluors in 2-Benzoxazolylfluorene, 2,5-Diphenyloxazoles, 1,3,5-Hexatrienes, 1,4-Distyrylbenzenes, and 2,7-Distyrylfluorenes (2002)

The relationship of structure to optical spectral properties was determined for five types of fluors in a search for an optimum-wavelength shifter to be used as part of the detection systems for high-energy particles from accelerators. In a search for photostable fluors to serve as waveshifters in plastic fibers it was found that the wavelengths of interest, absorption max 410 +/- 10 nm and fluorescence emission max 480 +/- 20 nm, along with other properties, such as high solubility and short fluorescence decay time, could be obtained from fluorophors composed of aromatic rings and vinyl groups only by using amino groups as auxochromes to give bathochromic shifts of wavelengths. Since primary, monoalkyl, and dialkylamino groups were not sufficiently photostable, a number of fluorophores bearing diarylamino groups were investigated. Syntheses of the fluors made use of the Buchwald amination, an improved version of the Emmons-Horner reaction, and other common reactions. The fluor types were the following: a 2-benzoxazolyl-7-(4-diarylamino)fluorene 7, 2-(4-cyanophenyl)-5-(4-aminophenyl)oxazoles 14 and 20, 1,3,5-hexatrienes 24a-d and 26a-c, 1,4-distyrylbenzenes 31d-g and 32a-e, and 2,7-distyrylfluorenes 40a,d-e. The unsymmetrical fluors 7, 14, and 20 were not as bright as the best hexatrienes, distyrylbenzenes, and distyrylfluorenes, which were all symmetrical. Where the 1,6-diaryl-1,3,5-hexatrienes 24a-d had high fluorescence quantum yield (Phi(f)), the 1,1,6,6-tetraryl-1,3,5-hexatrienes 26a-c had both lower epsilon and Phi(f). Where the 1,4-distyrylbenzenes 31d-g had high Phi(f), the 1,4-bis(2-phenylstyryl)benzenes 32a-e had Phi(f) = 0. Diarylamino groups as auxofluors conferred higher photochemical stability than dialkylamino groups on similar fluorophores. The 1,4-distyrylbenzenes 31d,e and the 2,7-distyrylfluorenes 40d,ehad the most desirable properties overall, which included fast decay times of 2 ns. Computer simulations predicted absorption and emission wavelengths fairly well, but were of little help for the prediction of brightness, stability, Phi(f), or decay time.
Zernike expansion of separable functions of Cartesian coordinates (2004)

A Zernike expansion over a circle is given for an arbitrary function of a single linear spatial coordinate. The example of a half-plane mask (Hilbert filter) is considered. The expansion can also be applied to cylindrical aberrations over a circular pupil. A product of two such series can thus be used to expand an arbitrary separable function of two Cartesian coordinates.
Electrochemical investigation of Substituted Pyranopyrazoles Adsorption on Mild Steel in Acid Solution (2012)

Four substituted pyranopyrazole derivatives (PPZs) were synthesized and their effects on the electrochemical behavior of mild steel in 1 M HCl were investigated using gravimetric measurements, Tafel extrapolation method, linear polarization resistance (LPR), and electrochemical impedance spectroscopy (EIS). Tafel polarization measurements revealed that these compounds effectively suppressed both the anodic and cathodic processes of mild steel corrosion in acid solution and acted as mixed-type inhibitors. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) examinations of electrode surface confirmed the existence of adherent layer of inhibitor on electrode surface. The UV–visible absorption spectrum of inhibitor solution containing mild steel indicated the formation of Fe–PPZ complex. Quantum chemical calculations have been used to evaluate the structural, electronic, and reactivity parameters of the selected pyranopyrazole derivatives in relation to their inhibition action.
LOAD TESTING, BENCHMARKING, AND APPLICATION PERFORMANCE MANAGEMENT FOR THE WEB (2002)

This paper discusses three important activities in this context: load testing, benchmarking, and application performance management. Best practices for each of these activities are identified. The paper also explains how basic performance results can be used to increase the efficiency of load testing procedures
Interleukin-6 Trans-Signaling and Colonic Cancer Associated with Inflammatory Bowel Disease (2012)

Interleukin-6 (IL-6) is a cytokine largely induced during infection, inflammation, and cancer. In the liver, IL-6 induces the synthesis of acute-phase proteins, which are believed to support the response of the body during infection and inflammation. Moreover, IL-6 has been reported to be a growth factor in multiple myeloma. IL-6 on cells binds to an IL-6 receptor (IL-6R) forming an IL-6/IL-6R complex, which associates with a homodimer of a second receptor subunit, gp130, to initiate signaling. Gp130 is present on all cells of the body, whereas IL-6R is only expressed on hepatocytes, some leukocytes, and some epithelial cells. Since gp130 has no measurable affinity for IL-6, cells which do not express IL-6R are unresponsive to the cytokine. A soluble form of IL-6R, which is found in the blood, can still bind IL-6 and the complex of IL-6/sIL-6R can bind to cellular gp130 also on cells without IL-6R expression. This signaling mechanism has been called trans-signaling. Interestingly, a soluble form of gp130 (sgp130) blocks IL-6 trans-signaling without affecting classic IL-6 signaling via the membrane-bound IL-6R. We used a dimerized version of sgp130 fused to the Fc portion of an IgG1 antibody (sgp130Fc) to discriminate between classic and trans-signaling of IL-6. It turned out that proinflammatory activities of IL-6 are mediated via trans-signaling, whereas anti-inflammatory or regenerative activities are mediated via classic signaling. These results are important for strategies to inhibit IL-6 signaling in autoimmune diseases such as rheumatoid arthritis, inflammatory bowel disease, and inflammation-associated colorectal cancer.
Power Management of Packet Switches via Differentiated Delay Targets (2008)

In this paper, we explore two novel scheduling algorithms which allow for both differentiated quality-of-service (QOS) and power conservation in input-queued packet switches. At their core is the idea of a backlog target which represents the delay sensitivity of each input/output port combination. The first algorithm, target-based projective cone scheduling (T- PCS), incorporates these targets into the well-studied projective cone scheduling algorithm, a generalized form of maximum weight matching (MWM). The second algorithm, average backlog scheduling(ABS), uses a 'memory window' to push average backlogs towards their targets. We explain the intuition behind each of these and then show, through simulation, that both exhibit high performance in terms of managing power and QOS, while simultaneously addressing these two key concerns in switches.
Relationship between chimpanzee Rh‐like genes and the R‐C‐E‐F blood group system (1993)

The antigenic closeness between the chimpanzee alloantigen Rc of the R-C-E-F system, and the human alloantigen Rho(D) suggests a phyloge-connection between their genes. To confirm at the molecular level the common origin of these genes, genomic DNA from 16 unrelated chimpanzees of various R-C-E-F phenotypes were digested by three restriction enzymes and analyzed by Southern blot using a human Rh cDNA probe and three exon-specific probes. Restrictions profiles displayed reach polymorphism. Correlations between some bands and certain R-C-E-F phenotypes demonstrate that the human Rh cDNA probe defines in chimpanzee genomic DNA some genes of the R-C-E-F system.
Effects of Indigestible Dextrine on Bowel Movements. (1993)

難消化性デキストリン (PF) の便通に及ぼす影響について検討する目的で, PF摂取後の糞便重量, 糞便水分量, 排便状態, 便性状, 胃腸症状等について調べた。1) PF10～60g摂取後の便性状は大半がバナナ状～半練状であり, 下痢発症のED50値は2.4g/kg体重と推定された。また, 臨床上問題となるような胃腸症状は認められなかった。2) PF35g/日, 5日の連続摂取で, 糞便重量, 排便回数の増加が認められたが, 糞便水分含有率は変化がみられなかった。3) PF5～10g/日, 5日の連続摂取で, 排便状態の改善が認められた。
Lysis of Erythrocytes by Silicate Minerals (1986)

Abstract In vitro studies of the destruction (lysis) of bovine red blood cells (erythrocytes) by some silicate minerals showed the reaction to be complete in less than 1 hr and very destructive to the cell membrane. The activity as lysing agents was found to be in the order smectites > silica > palygorskite ≃ sepiolite > chrysotile > kaolinite. Different compositions (Fe, Al, Mg, Li, vacancy) of the octahedral sheet of the smectite and fibrous clay minerals did not appreciably alter their hemolytic activity. The most active particle size range for kaolinite and montmorillonite was 0.2-2 μ m. Structural folding of palygorskite reduced lysis suggesting that edge surfaces and silanol groups are important in this process. Aluminum oxides and hydroxides caused no lysis, and coatings of positively charged aluminum-hydroxy polymers on montmorillonite, silica, palygorskite, and kaolinite significantly reduced lysis.
An Overview on Concept Drift Learning (2018)

Concept drift techniques aim at learning patterns from data streams that may change over time. Although such behavior is not usually expected in controlled environments, real-world scenarios can face changes in the data, such as new classes, clusters, and features. Traditional classifiers can be easily fooled in such situations, resulting in poor performances. Common concept drift domains include recommendation systems, energy consumption, artificial intelligence systems with dynamic environment interaction, and biomedical signal analysis (e.g., neurogenerative diseases). In this paper, we surveyed several works that deal with concept drift, as well as we presented a comprehensive study of public synthetic and real datasets that can be used to cope with such a problem. In addition, we considered a review of different types of drifts and approaches to handling such changes in the data. We considered different learners employed in classification tasks and the use of drift detection mechanisms, among other characteristics.
Production and characterization of recombinant chicken insulin-like growth factor-I from Escherichia coli (1992)

Recombinant chicken insulin-like growth factor-I (cIGF-I) has been produced in Escherichia coli after first modifying a plasmid that coded for a human IGF-I (hIGF-I) fusion protein, in order to introduce codons for the eight amino acid substitutions. The cIGF-I fusion protein, deposited in bacterial inclusion bodies, was dissolved under reducing conditions, desalted, subjected to anion-exchange chromatography to remove proteinases, refolded and partially purified by reverse-phase high-performance liquid chromatography. The fusion protein was cleaved with hydroxylamine after which cIGF-I was purified to homogeneity by three additional chromatographic steps. Recombinant cIGF-I was equipotent with hIGF-I in cell culture bioassays of protein synthesis and breakdown using rat L6 myoblasts and chick embryo fibroblasts. Binding of radiolabelled cIGF-I and hIGF-I was also equivalent in the two cell lines, as was their binding in ligand blots of chicken, sheep and human plasma. The cross-reactivity of cIGF-I in a polyclonal hIGF-I radioimmunoassay was 60% of that observed with hIGF-I. The availability of recombinant cIGF-I will facilitate investigations into the role of IGF-I in chicken growth and development.
Identifying Cyberbullying, Connecting with Students: The Promising Possibilities of Teacher-Student Social Networking (2012)

Cyberbullying, an emergent problem that most students face but few report, negatively affects students’ academic and personal development, disrupts the school environment, and usually peaks around middle school. The Association of Middle Level Education (AMLE) suggests that successful middle schools should, among other things, ensure every student has an adult advocate to guide academic and personal development in an inviting, safe, inclusive, and supportive school environment. The Olweus Anti-Bullying Program denotes educators’ proactive intervention must first follow recognition of students’ misbehaviors and both identification and supervision of problematic school contexts. Without such recognition, identification, and supervision, educators’ proactive interventions are likely impossible. This article offers social networking to educators as a method to identify and, to the best extent possible, supervise cyberbullying. This identification and supervision method merges with youth culture and coheres with AMLE’s and Olweus’ philosophies to positively influence the school’s environment and facilitate students’ intellectual and personal development. However, it contrasts sharply with various school districts’ approaches to confronting cyberbullying. The authors intend for this premise to spark interest in potential pilot studies whereby educators conscientiously and deliberately construct a path to proactive intervention.
3D Object Detection from LiDAR Data using Distance Dependent Feature Extraction (2020)

This paper presents a new approach to 3D object detection that leverages the properties of the data obtained by a LiDAR sensor. State-of-the-art detectors use neural network architectures based on assumptions valid for camera images. However, point clouds obtained from LiDAR are fundamentally different. Most detectors use shared filter kernels to extract features which do not take into account the range dependent nature of the point cloud features. To show this, different detectors are trained on two splits of the KITTI dataset: close range (objects up to 25 meters from LiDAR) and long-range. Top view images are generated from point clouds as input for the networks. Combined results outperform the baseline network trained on the full dataset with a single backbone. Additional research compares the effect of using different input features when converting the point cloud to image. The results indicate that the network focuses on the shape and structure of the objects, rather than exact values of the input. This work proposes an improvement for 3D object detectors by taking into account the properties of LiDAR point clouds over distance. Results show that training separate networks for close-range and long-range objects boosts performance for all KITTI benchmark difficulties.
Market-based user innovation in the Internet of Things (2010)

The importance of user innovation is widely accepted, but the development of the Internet of Things is primarily driven by large commercial players. Using an innovation perspective, this paper identifies how user innovation and market-based innovation can be combined in the Internet of Things (IoT). A survey of tools for user/developers in the IoT space uncovers a rich set of tools for creation of hardware, software and data but reveals poor support for distribution and sharing of such artifacts. To address this shortcoming we propose connected marketplaces as a way to provide users/developers with rich opportunities for sharing and trading of artifacts, and to enable effective user innovation in the IoT.
Interaction between interstitial cells and smooth muscles in the lower urinary tract and penis (2006)

Smooth muscles in the lower urinary tract and corporal tissue exhibit spontaneous contractile activity which depends on L-type Ca(2+) channels. The mechanism underlying this activity is spontaneous electrical activity which shows varied form and property between these tissues. Recent studies revealed that interstitial cells (ICs) are widely distributed in the genitourinary system, and suggested their involvement in spontaneous muscle activity. ICs in the system are not a simple analogy of interstitial cells of Cajal (ICC) in the gut, which act as electrical pacemaker, but represent variability amongst tissues which may account for individual characteristics of each organ. In the bladder and corporal tissue, where smooth muscle cells are capable of generating spontaneous electrical activity, ICs may modulate smooth muscle activity. ICs in corporal tissue release prostaglandins via cyclooxygenase-2 (COX-2) activity and reinforce not only spontaneous but also nerve-mediated alpha-adrenergic contractions. In the bladder, their fundamental role in the integration of signals between populations of cells has been proposed, and thus changes in ICs may contribute to an overactive bladder, a pathological condition which results from increased excitability in detrusor smooth muscles. In the urethra, ICs may act as electrical pacemakers as do ICC. However, overall contractility of urethral smooth muscles does not necessarily rely on pacemaking of ICs, and thus some population of smooth muscles may also have their own excitability.
Pharmacological Properties of Foods and Nutrients (2000)

SUMMARY Nutrients, i.e., protein, carbohydrate, fat, fiber, vitamins, minerals, trace elements, and water, are commonly viewed as primary modulators of health and disease, yet they also contain many bioactive substances or “extranutritional” components. These occur naturally, are produced in vivo, or may be produced by the industrial enzymatic digestion related to various food processing activities. While these substances have been viewed as inconsequential in relation to human health, a wide range of such food constituents are recognized as having multifunctional roles in health and disease arising from the physiological, behavioral and/or immuniologic influence(s) they exert.
Addition of Antioxidant of Bamboo Leaves (AOB) Effectively Reduces Acrylamide Formation in Potato Crisps and French Fries (2006)

The present study was to demonstrate the efficiency of antioxidant of bamboo leaves (AOB) on the reduction of acrylamide during thermal processing and to summarize the optimal level of AOB applied in potato-based products. Potato crisps and French fries were immersed into different contents of AOB solution, and the frying processing parameters were optimized. The acrylamide content was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The sensory evaluation was performed in double blind manner. Our results showed that nearly 74.1% and 76.1% of acrylamide in potato crisps and French fries was reduced when the AOB addition ratio was 0.1% and 0.01% (w/w), respectively. The maximum inhibitory rate was achieved when the immersion time was designed as 60 s. Sensory evaluation results showed that the crispness and flavor of potato crisps and French fries processed by AOB solution had no significant difference compared to normal potato matrixes (P > 0.05) when the AOB addition ratio was <0.5% (w/w). These results suggested that AOB could significantly reduce acrylamide formation in potato-based foods and keep original crispness and flavor of potato matrixes. This study could be regarded as a pioneer contribution on the reduction of acrylamide in various foods by natural antioxidants.