gebhart/patrepeval huggingface dataset:
A Tyrosine-to-Threonine Mutation Converts Cycloartenol Synthase to an Oxidosqualene Cyclase that Forms Lanosterol as Its Major Product (2000)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Tyrosine-to-Threonine Mutation Converts Cycloartenol Synthase to an Oxidosqualene Cyclase that Forms Lanosterol as Its Major ProductJennifer B. R. Herrera, William K. Wilson, and Seiichi P. T. MatsudaView Author Information Department of Chemistry and Department of Biochemistry and Cell Biology Rice University, Houston, Texas 77005 Cite this: J. Am. Chem. Soc. 2000, 122, 28, 6765–6766Publication Date (Web):June 28, 2000Publication History Received17 April 2000Published online28 June 2000Published inissue 1 July 2000https://pubs.acs.org/doi/10.1021/ja0013226https://doi.org/10.1021/ja0013226rapid-communicationACS PublicationsCopyright © 2000 American Chemical SocietyRequest reuse permissionsArticle Views519Altmetric-Citations52LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Cations,Genetics,Organic compounds,Peptides and proteins,Triterpenes Get e-Alerts
II. Derivate des Prolins (1908)

3 e i der tryptischen Verdauung der Gelatine haben L e v e n e und B e a t t y l ) ein Prolylglycinanhydrid isolirt,
Static and Dynamic Studies of Electro-Active Polymer Actuators and Integration in a Demonstrator (2017)

Nowadays, the haptic effect is used and developed for many applications—particularly in the automotive industry, where the mechanical feedback induced by a haptic system enables the user to receive information while their attention is kept on the road and on driving. This article presents the development of a vibrotactile button based on printed piezoelectric polymer actuation. Firstly, the characterization of the electro-active polymer used as the actuator and the development of a model able to predict the electromechanical behavior of this device are summarized. Then, the design of circular membranes and their dynamic characterization are presented. Finally, this work is concluded with the construction of a fully functional demonstrator, integrating haptic buttons leading to a clear haptic sensation for the user.
Processing of sulfonated polysulfone for PEMFC membrane applications: Part 2. Polymer in salt form (2006)

Abstract The possibility of manufacturing sulfonated polysulfone (sPSU) membranes by an extrusion process is explored for low‐temperature proton exchange membrane fuel cell applications. Because the acid form of sPSU is not suitable for melt‐phase processing, the use of the salt form of the polymer was considered, as sulfonate groups are much less reactive than sulfonic acid groups. This form of sPSU makes it possible to use an extrusion process with the help of a suitable plasticizer, such as poly(ethyleneglycol) (PEG). The residual porosity of the film, induced by removing the soluble PEG with water, was found to improve the proton conductivity of the membrane. The membrane fabrication process has a prominent effect on the final properties of the film. For the same composition, a solvent‐casting procedure can produce membranes with proton conductivity twice as high as that attainable with a melt‐phase process. However, extruded membranes exhibit a greater longevity than do cast membranes. At the same time, the proton conductivity of extruded membranes is less dependent on the ion‐exchange capacity of the polymer. © 2006 Wiley Periodicals, Inc. Adv Polym Techn 25: 127–133, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/adv.20067
Regulation of the alpha 1(I) collagen promoter via a transforming growth factor-beta activation element (1993)

A transforming growth factor-beta (TGF-beta) activating element (TAE), with a nuclear factor-1 (NF-1)-like sequence, was previously located 1.6 kilobases upstream from the transcription start site in the alpha 1(I) collagen promoter (Ritzenthaler, J. D., Goldstein, R. H., Fine, A., Lichtler, A., Rowe, D. W., and Smith, B. D. (1991) Biochem. J. 280, 157-162). Double-stranded TAE, but not NF-1 consensus sequences, abrogated TGF-beta stimulation of co-transfected collagen promoter-chloramphenicol acetyltransferase constructs. Mutations in non-NF-1 binding sites, located by methylation interference, eliminated activity of the TAE oligonucleotide. However, TAE sequences failed to bind in vitro expressed NF-1 protein, to compete for NF-1-binding proteins, and to bind with protein which reacts with antibodies to NF-1 family of proteins. Within the TAE there was an activator protein 2 (AP-2) binding site. Although AP-2 protein bound to TAE, antibodies to AP-2 did not react with nuclear protein-TAE complexes. TAE bound to a 34,000-Da protein on Southwestern analysis. However, the UV-cross-linked TAE-nuclear protein complex was 82,000 Da. Finally, a dose-response study demonstrated that TGF-beta increased TAE nuclear binding proteins at lower doses with a different response curve than NF-1 nuclear binding proteins. Taken together these data demonstrated that TGF-beta functions in human lung fibroblasts to activate collagen transcription through TAE sites by protein complexes independent of NF-1 or AP-2 protein.
Eye position effects on visual, memory, and saccade-related activity in areas LIP and 7a of macaque (1990)

We studied the effect of eye position on the light-sensitive, memory, and saccade-related activities of neurons of the lateral intraparietal area and area 7a in the posterior parietal cortex of rhesus monkeys. A majority of the cells showed significant effects of eye position, for each of the 3 types of response. The direction tuning of the light-sensitive, memory and saccade responses did not change with eye position but the magnitude of the response did. Since previous work showed a similar effect for the light-sensitive response of area 7a neurons (Andersen and Mountcastle, 1983; Andersen et al., 1985b), the present results indicate that this modulating effect of eye position may be a general one, as it is found in 3 types of responses in 2 cortical areas. Gain fields were mapped by measuring the effect of eye position on the magnitude of the response at 9 different eye positions for each neuron. The gain fields were usually planar or largely planar for all 3 types of response in both areas, indicating that the magnitude of the response usually varies linearly with both horizontal and vertical eye position. A similar observation was made previously for the gain fields of the light-sensitive response of area 7a neurons (Andersen et al., 1985b). Although gain fields sloped in all directions for the population of cells, the gain field slopes of the light-sensitive, memory and saccade responses for individual cells were usually similar. It is proposed that these eye position effects play an important role in making coordinate transformations for visually guided movement.
Aerothermal Study of Intermediate Turbine Ducts (2016)

An intermediate turbine duct guides the flow from the smaller diameter high pressure turbine to the downstream larger diameter intermediate or low pressure turbine. It is typically equipped with a structural vane transferring loads for the core to the external parts, and supplies the core with necessary services such as oil and air. Flow in an intermediate turbine duct is highly complex, influenced by the upstream turbine stage flow structures, which include tip leakage flows and non-uniformities originating from the upstream high pressure turbine vane and rotor. The complexity of the flow structures makes predicting them using numerical methods difficult, hence there exists a need for experimental validation. This thesis includes results from experiments conducted in two different facilities, the Chalmers Large-Scale Low-Speed Turbine Facility and the Oxford Turbine Research Facility. At Chalmers, the emphasis was at aerodynamic measurements including static pressure, total pressure, velocities and flow angles in the intermediate turbine duct. In Oxford, the experimental campaign included both aerodynamic and heat transfer measurements with the purpose of describing the aerothermal flow through the intermediate turbine duct. A new intermediate turbine duct with a medium turning vane was designed, manufactured, instrumented and installed in the Oxford Turbine Research Facility. Three high pressure turbine stage inlet conditions were studied; a uniform inlet flow and two low-NOx swirl profiles. Three adjacent intermediate turbine duct vanes were instrumented in an identical manner, to study the vane-to-vane clocking effect. Instrumentation includes static pressure tappings and thin film heat flux gauges installed on the intermediate turbine duct vane and endwalls at discrete locations. A three-hole pressure probe equipped with thermocouples above and below was traversed at the intermediate turbine duct exit. Steady and unsteady CFD predictions were performed of the intermediate turbine duct on its own and the full 1.5 high pressure turbine stage. The results from the test campaign in Oxford show the clocking effect to have a larger effect on the surface aerodynamics and heat transfer of the intermediate turbine duct vane than the introduction of a low-NOx swirl profile at the high pressure turbine stage inlet. The clocking effect is for example seen to change the incidence to the intermediate turbine duct vane. The change in inlet condition is seen to have local effects in the intermediate turbine duct. The CFD results are found to in general match the experimental data fairly well, both in aerodynamics and heat transfer. A comparison of steady and averaged time averaged unsteady CFD is noted to provide similar results.
Preoperative Roentgenographically Guided Percutaneous Localization of Occult Breast Lesions (1982)

The identification of a suspicious occult breast lesion with mammography should, in most instances, result in roentgenographically guided localization followed by surgical excision. Over a three-year period, we performed 180 roentgenographically guided preoperative localizations for occult breast lesions, 32% (57/180) of which were malignant. Of the total, there were 85 masses and 95 clustered microcalcifications. We prefer to use a modified hookwire for lesion localization.
A scalable framework for heterogeneous GPU-based clusters (2012)

GPU-based heterogeneous clusters continue to draw attention from vendors and HPC users due to their high energy efficiency and much improved single-node computational performance, however, there is little parallel software available that can utilize all CPU cores and all GPUs on the heterogeneous system efficiently. On a heterogeneous cluster, the performance of a GPU (or a compute node) increases in a much faster rate than the performance of the PCI-Express connection (or the interconnection network) such that communication eventually becomes the bottleneck of the entire system. To overcome the bottleneck, we developed a multi-level partitioning and distribution method that guarantees a near-optimal communication volume. We have also extended heterogeneous tile algorithms to work on distributed memory GPU clusters. Our main idea is to execute a serial program and generate hybrid-size tasks, and follow a dataflow programming model to fire the tasks on different compute nodes. We then devised a distributed dynamic scheduling runtime system to schedule tasks, and transfer data between hybrid CPU-GPU compute nodes transparently. The runtime system employs a novel distributed task-assignment protocol to solve data dependencies between tasks without coordination between processing units. The runtime system on each node consists of a number of CPU compute threads, a number of GPU compute threads, a task generation thread, an MPI communication thread, and a CUDA communication thread. By overlapping computation and communication through dynamic scheduling, we are able to attain a high performance of 75 TFlops for Cholesky factorization on the heterogeneous Keeneland system using 100 nodes, each with twelve CPU cores and three GPUs. Moreover, our framework is able to attain high performance on distributed-memory clusters without GPUs, and shared-system multiGPUs.
The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells. (1995)

We have identified a Schizosaccharomyces pombe gene with homology to the budding yeast gene CDC5, the Drosophila gene polo, and the mammalian family of genes encoding polo-like kinases. Disruption of this gene, plo1+, indicates that it is essential. Loss of plo1+ function leads to a mitotic arrest in which condensed chromosomes are associated with a monopolar spindle or to the failure of septation following the completion of nuclear division. In the latter case, cells show a failure both in the formation of an F-actin ring and in the deposition of septal material, suggesting that plo1+ function is required high in the regulatory cascade that controls septation. The overexpression of plo1+ in wild-type cells also results in the formation of monopolar spindles but also induces the formation of multiple septa without nuclear division. Septation can also be induced in the absence of mitotic commitment and concomitant spindle formation by the overexpression of plo1+ in cdc25-22 or cdc2-33 cells arrested in G2; in G1 cells arrested at Start by the cdc10-V50 mutation, or in cells lacking the cyclin B homolog cdc13 that undergo repeated S phases in the absence of mitosis.
Fast algorithms for nonconvex compressive sensing: MRI reconstruction from very few data (2009)

Compressive sensing is the reconstruction of sparse images or signals from very few samples, by means of solving a tractable optimization problem. In the context of MRI, this can allow reconstruction from many fewer k-space samples, thereby reducing scanning time. Previous work has shown that nonconvex optimization reduces still further the number of samples required for reconstruction, while still being tractable. In this work, we extend recent Fourier-based algorithms for convex optimization to the nonconvex setting, and obtain methods that combine the reconstruction abilities of previous nonconvex approaches with the computational speed of state-of-the-art convex methods.
Aniline-Catalyzed Reductive Amination as a Powerful Method for the Preparation of Reducing End-“Clickable” Chitooligosaccharides (2013)

Functionalized oligosaccharides are useful intermediates to prepare products for biological research or for the development of advanced functional materials. Here, we report the unprecedented use of aniline as an efficient organocatalyst reaction with "clickable" (azide or alkyne) amine for the transimination-mediated reductive amination of a chitooligosaccharide. Moreover, we demonstrate that alkyne-bearing aniline constitutes an excellent tool for the easy derivatization of chitosan oligosaccharides. Evidence for such improvement has been illustrated by the straightforward design of a FRET substrate to probe chitinase activity and of amphiphilic polycaprolactone-grafted-chitosan. This efficient methodology paves the way to the preparation of novel chitosan oligosaccharide-based advanced materials.
Design, synthesis and evaluation of 2-(1,3-dioxoisoindolin-2-yl)-Nphenylacetamides as inhibitors of reverse transcriptase (2013)

In the present study, we have designed and synthesized 16 novel 1,3-dioxoisoindole 4(a-p) derivatives as HIV-1 Non-Nucleoside Reverse Transcriptase Inhibitors. Lipinski rule of five parameters and molecular parameters like drug likeness and drug scores were calculated for the designed analogs using online servers like Molinspiration and Osiris property explorer. Designed analogs were synthesized using a known synthetic protocol. Synthesized compounds were characterized using FT-IR 1H NMR Mass and Elemental analysis. The compounds were evaluated for their HIV-1 Reverse Transcriptase (RT) inhibitor activity by HIV-1 RNA dependent DNA polymerase activity assay at 2 and 20μM concentrations. Among the designed analogs, 4a, 4c, 4d, 4h, 4l, 4m and 4p showed weak Reverse Transcriptase inhibitory activity at 20μM concentration. For the designed compounds, there was no correlation observed between molecular modeling and in-vitro studies.
Water Treatment with Conventional and Alternative Coagulants (2017)

Half of the world's population does not have access to basic sanitation, leading to socio-economic problems, such as the lack of drinking water and the spread of diseases. In this way, the development of water treatment technologies that are accessible to that target people is of utmost relevance. Currently, in the water treatment of separation process, the compound mostly used as a coagulant in water treatment is the aluminum sulphate, which gives good results for turbidity and color removal of raw water. However, studies show that its deposition in the human body can cause serious damage to health, and the development of diseases, including Alzheimer's disease. Thus, this project set out to do a parallel study between the aluminum sulphate coagulant and as an alternative, the seed extract of Moringa oleífera (MO). The efficiency of both coagulants were evaluated through measurement of turbidity and color of water samples. MO proved to be more efficient, with removals of 94.9% of turbidity and 92.5% of color, when using a dosage of 20 mg L-1. The water obtained results close to drinking water required by Brazilian regulation, indicating that MO seed could be an alternative for water treatment providing water for small communities.
Identification and assessment of BWR in-vessel severe accident mitigation strategies (1992)

This report provides the results of work carried out in support of the US Nuclear Regulatory Commission Accident Management Research Program to develop a technical basis for evaluating the effectiveness and feasibility of current and proposed strategies for boiling water reactor (BWR) severe accident management. First, the findings of an assessment of the current status of accident management strategies for the mitigation of in-vessel events for BWR severe accident sequences are described. This includes a review of the BWR Owners` Group Emergency Procedure Guidelines (EPGSs) to determine the extent to which they currently address the characteristic events of an unmitigated severe accident and to provide the basis for recommendations for enhancement of accident management procedures. Second, where considered necessary, new candidate accident management strategies are proposed for mitigation of the late-phase (after core damage has occurred) events. Finally, recommendations are made for consideration of additional strategies where warranted, and two of the four candidate strategies identified by this effort are assessed in detail: (1) preparation of a boron solution for reactor vessel refill should control blade damage occur during a period of temporary core dryout and (2) containment flooding to maintain the core debris within the reactor vessel if the injection systems cannot be restored.
Tools used to study how protein complexes are assembled in signaling cascades (2011)

Most proteins do not function on their own but as part of large signaling complexes that are arranged in every living cell in response to specific environmental cues. Proteins interact with each other either constitutively or transiently and do so with different affinity. When identifying the role played by a protein inside a cell, it is essential to define its particular cohort of binding partners so that the researcher can predict what signaling pathways the protein is engaged in. Once identified and confirmed, the information might allow the interaction to be manipulated by pharmacological inhibitors to help fight disease. In this review, we discuss protein-protein interactions and how they are essential to propagate signals in signaling pathways. We examine some of the high-throughput screening methods and focus on the methods used to confirm specific protein-protein interactions including; affinity tagging, co-immunoprecipitation, peptide array technology and fluorescence microscopy.
Global Optimization of Nonlinear Blend-Scheduling Problems (2017)

The scheduling of gasoline-blending operations is an important problem in the oil refining industry. This problem not only exhibits the combinatorial nature that is intrinsic to scheduling problems, but also non-convex nonlinear behavior, due to the blending of various materials with different quality properties. In this work, a global optimization algorithm is proposed to solve a previously published continuous-time mixed-integer nonlinear scheduling model for gasoline blending. The model includes blend recipe optimization, the distribution problem, and several important operational features and constraints. The algorithm employs piecewise McCormick relaxation (PMCR) and normalized multiparametric disaggregation technique (NMDT) to compute estimates of the global optimum. These techniques partition the domain of one of the variables in a bilinear term and generate convex relaxations for each partition. By increasing the number of partitions and reducing the domain of the variables, the algorithm is able to refine the estimates of the global solution. The algorithm is compared to two commercial global solvers and two heuristic methods by solving four examples from the literature. Results show that the proposed global optimization algorithm performs on par with commercial solvers but is not as fast as heuristic approaches.
Total nucleated cell differential for blood and bone marrow using a single tube in a five‐color flow cytometer (2007)

We have designed a one-tube immunophenotyping panel for classification of total nucleated cells and platelets in blood or bone marrow. The seven parameters available in one single tube in our cytometer seem to be enough for reliable differential count even in difficult pathological samples. The analytical imprecision of the flow cytometer differential was much lower than that obtained with microscopy or cell counter differentials.
CORRECTION OF HUMP OR SADDLE BRIDGE NOSE: SIMPLIFIED METHOD (1925)

The method that I report is, I believe, an original surgical procedure for the correction of hump and saddle bridge noses and of some other nasal deformities. It is one which I have practiced for the last six years. I described its technic first while on the board the S. S.<i>Rotterdam</i>on June 29, 1922, to Dr. George W. Mackenzie, of Philadelphia, and a group of eye, ear, nose and throat specialists which he conducted to Vienna for a course of study. On July 18, 1924, I again described the operative procedure at a meeting of the Venango County Medical Society of Pennsylvania. I outline this operation because of its facility and safety, for the greatest deterrent to the correction of external nasal deformities is the difficulty of reaching the field without causing infection or scar formation. The removal of the enlargement in hump nose, or the