gebhart/patrepeval huggingface dataset:
Enhancement of the Immunostimulatory Functions of Ex Vivo–Generated Dendritic Cells from Early-Stage Colon Cancer Patients by Consecutive Exposure to Low Doses of Sequential-Kinetic-Activated IL-4 and IL-12. A Preliminary Study (2015)

Dendritic cells (DCs), specialized antigen-presenting cells bridging innate and adaptive immunity, play a crucial role in determining specific immune response to tumors. Because of their potent immunoregulatory capacities, DCs have been exploited in anticancer vaccination, with limited success thus far. This pilot study compared low-dose interleukin (IL)-4 and IL-12 prepared by sequential kinetic activation (SKA) with standard doses of the same recombinant human cytokines on functional activity of ex vivo-generated monocyte-derived (Mo) DCs from colon carcinoma patients and normal subjects. MoDCs were exposed to medium alone, SKA-IL-4 (0.5 fg/ml), or SKA-IL-12 (2 fg/ml), alone or consecutively combined, in parallel with rhIL-4 (50 ng/ml) and rhIL-12 (1 ng/ml). Primary allogeneic one-way mixed lymphocyte reaction (MLR) was the end point to assess in vitro T-lymphocyte proliferation in response to MoDCs, and secreted IL-12p70 and interferon-γ in MLR supernatants measured by ELISA to assay for T-helper 1-promoting MoDC phenotype. No single agent enhanced the compromised allostimulatory activity of MoDCs from colon cancer patients, unlike healthy donors. However, MoDCs from nonmetastatic colon cancer patients, after sequential exposure to SKA-IL-4 (48 hours) and SKA-IL-12 (24 hours), displayed increased T-cell stimulatory capacity by MLR and acquired driving T-helper 1 polarization activity, although less markedly than the effects induced by recombinant human cytokines or found in normal subjects. These results point to an immunomodulatory capacity of low-dose SKA-IL-4 and SKA-IL-12 and encourage further investigation to provide clues for the rational development of new and more effective immunotherapeutic strategies against cancer.
Vascular endothelial growth factor: Direct neuroprotective effect in <i>in vitro</i> ischemia (2000)

Vascular endothelial growth factor (VEGF) is a hypoxia-inducible angiogenic peptide with recently identified neurotrophic effects. Because some neurotrophic factors can protect neurons from hypoxic or ischemic injury, we investigated the possibility that VEGF has similar neuroprotective properties. In HN33, an immortalized hippocampal neuronal cell line, VEGF reduced cell death associated with an in vitro model of cerebral ischemia: at a maximally effective concentration of 50 ng/ml, VEGF approximately doubled the number of cells surviving after 24 h of hypoxia and glucose deprivation. To investigate the mechanism of neuroprotection by VEGF, the expression of known target receptors for VEGF was measured by Western blotting, which showed that HN33 cells expressed VEGFR-2 receptors and neuropilin-1, but not VEGFR-1 receptors. The neuropilin-1 ligand placenta growth factor-2 failed to reproduce the protective effect of VEGF, pointing to VEGFR-2 as the site of VEGF's neuroprotective action. Two phosphatidylinositol 3'-kinase inhibitors, wortmannin and LY294002, reversed the neuroprotective effect of VEGF, implicating the phosphatidylinositol 3'-kinase/Akt signal transduction system in VEGF-mediated neuroprotection. VEGF also protected primary cultures of rat cerebral cortical neurons from hypoxia and glucose deprivation. We conclude that in addition to its known role as an angiogenic factor, VEGF may exert a direct neuroprotective effect in hypoxic-ischemic injury.
Lythraceous alkaloids. X. Alkaloids of Lagerstroemia subcostata and L. favriei: A contribution to the chemotaxonomy. (1978)

The basic components of Lagerstroemia subcostata collected on Amami-ohshima Island and two slightly different types of Lagerstroemia fauriei collected on Tanegashima Island were investigated. We distinguished the two types by calling them L. fauriei and L. fauriei (Tanegashima-type). Four new alkaloids, lasubine-I (12), -II (14), subcosine-I (15), and -II (16), all of which belong to type E, were isolated from the former. From the latter two, however, lythrine (3), cryogenine (17), and lythridine (18), all of which belong to type C, were isolated. L. fauriei has been classified as L. subcostata var. fauriei by S. Hatusima, but from the chemotaxonomical aspect, it shows prominent differences from L. subcostata. Thus their classification under species would be more appropriate.
Parkinson's Disease Metabolic Network Predicts Phenoconversion in Patients with Idiopathic REM Sleep Behavior Disorder (S19.004) (2013)

OBJECTIVE: To determine whether expression of the Parkinson's disease-related metabolic pattern (PDRP) predicts neurodegenerative disease in patients with idiopathic REM sleep behavior disorder (iRBD).
Differential regulation of the coexpressed calcitonin/alpha-CGRP and beta-CGRP neuroendocrine genes. (1988)

The rat calcitonin/alpha-CGRP and beta-CGRP genes are members of a neuroendocrine gene family that are coexpressed in a distinct cell-specific pattern. The alpha- and beta-CGRP gene sequences predict highly related peptides that differ by only a single amino acid. Using rat medullary thyroid carcinoma cells as an in vitro system for testing hormone responsiveness, we have shown that the calcitonin/alpha-CGRP and beta-CGRP genes are differentially regulated. The synthetic glucocorticoid, dexamethasone, caused a 2- to 5-fold increase in calcitonin and alpha-CGRP mRNAs due to an increase in the nuclear transcription rate. In contrast, the levels of beta-CGRP mRNA did not significantly change following dexamethasone treatment. Differential regulation within neuroendocrine gene families might be a common mechanism for generating functionally similar peptides in response to a variety of hormonal signals.
Heat Transfer in Health and Healing1 (2015)

Our bodies depend on an exquisitely sensitive and refined temperature control system to maintain a state of health and homeostasis. The exceptionally broad range of physical activities that humans engage in and the diverse array of environmental conditions we face require remarkable strategies and mechanisms for regulating internal and external heat transfer processes. On the occasions for which the body suffers trauma, therapeutic temperature modulation is often the approach of choice for reversing injury and inflammation and launching a cascade of healing. The focus of human thermoregulation is maintenance of the body core temperature within a tight range of values, even as internal rates of energy generation may vary over an order of magnitude, environmental convection, and radiation heat loads may undergo large changes in the absence of any significant personal control, surface insulation may be added or removed, all occurring while the body's internal thermostat follows a diurnal circadian cycle that may be altered by illness and anesthetic agents. An advanced level of understanding of the complex physiological function and control of the human body may be combined with skill in heat transfer analysis and design to develop life-saving and injury-healing medical devices. This paper will describe some of the challenges and conquests the author has experienced related to the practice of heat transfer for maintenance of health and enhancement of healing processes.
Dynamic Infinite Relational Model for Time-varying Relational Data Analysis (2010)

We propose a new probabilistic model for analyzing dynamic evolutions of relational data, such as additions, deletions and split &amp; merge, of relation clusters like communities in social networks. Our proposed model abstracts observed timevarying object-object relationships into relationships between object clusters. We extend the infinite Hidden Markov model to follow dynamic and time-sensitive changes in the structure of the relational data and to estimate a number of clusters simultaneously. We show the usefulness of the model through experiments with synthetic and real-world data sets. 1
Efficient Production of Block-copolymer-coated Ceramic Nanoparticles by Sequential Reversible Addition–Fragmentation Chain-transfer Polymerizations with Particles (SqRAFTwP) (2013)

Abstract Ceramic nanoparticles (NPs) were surface modified and dispersed by in situ synthesized block copolymers. Living radical polymerization with sequential addition of monomers enables the effective coating of ceramic NPs by block copolymers. The method enables us to add stable polymer layers on any kind of NPs; therefore, it can help the mass production of polymer-coated inorganic NPs and drastically reduce the cost, without sacrificing their performance.
PLASMA DESORPTION MASS SPECTROMETRY STUDIES OF SMALL PROTEINS ADSORBED TO NITROCELLULOSE (1989)

Adsorption of bovine insulin to nitrocellulose has been studied using Plasma Desorption Mass Spectrometry (PDMS) and ellipsometry. The results indicate that PDMS can be used to study competition between adsorption of insulin to nitrocellulose and self association.
Destruction of Food Spoilage, Indicator and Pathogenic Organisms by Various Germicides in Solution and on a Stainless Steel Surface1 (1976)

Representative food spoilage, indicator, and pathogenic organisms were exposed to newly developed imported iodophors and widely used representative domestic germicides that included iodophors. The organisms used in these experiments included Salmonella derby, Escherichia coli, yeast of the genus Candida, Pseudomonas aeruginosa, Staphylococcus aureus. Streptococcus lactis, and spore of Bacillus licheniformis. Results of the germicide experiments showed generally similar effectiveness by iodophor and hypochlorite. Both were superior to the quaternary ammonium compounds (QAC) when used at much lower concentrations (12.5 and 25 for the halogens and 50 and 200 ppm for the QAC). The iodophors exhibited a consistently greater rate of destruction of yeast cells than the hypochlorite when low concentrations of germicide were used. A procedure to more closely simulate actual use of germicides in food and dairy industries also was employed in this study. Special polished metal strips were inoculated with organisms and exposed to an iodophor, a sodium hypochlorite, and a QAC for 15, 30, 60, and 300 sec. Again the halogen compounds were superior to the QAC in destroying the bacterial cells, especially against gram-negative species including Salmonella.
Mechanism of action of spiramycin and other macrolides (1988)

Macrolide antibiotics constitute a group of 12 to 16-membered lactone rings substituted with one or more sugar residues, some of which may be amino sugars. They inhibit bacterial protein synthesis both in vivo and in vitro with varying potencies. Macrolides are generally bacteriostatic, although some of these drugs may be bactericidal at very high concentrations. The mechanism of action of macrolides has been a matter of controversy for some time. Spiramycin, a 16-membered macrolide, inhibits translocation by binding to bacterial 50S ribosomal subunits with an apparent 1:1 stoichiometry. This antibiotic is a potent inhibitor of the binding to the ribosome of both donor and acceptor substrates. Spiramycin induces rapid breakdown of polyribosomes, an effect which has formerly been interpreted as occurring by normal ribosomal run-off followed by an antibiotic-induced block at or shortly after initiation of a new peptide. However, there is now convincing evidence that spiramycin, and probably all macrolides, act primarily by stimulating the dissociation of peptidyl-tRNA from ribosomes during translocation. Although the ribosomes of both Gram-positive and Gram-negative organisms are susceptible to macrolides, these antibiotics are mainly used against Gram-positive bacteria since they are unable to enter the porins of Gram-negative bacteria. Resistance to macrolides in clinical isolates is most frequently due to post-transcriptional methylation of an adenine residue of 23S ribosomal RNA, which leads to co-resistance to macrolides, lincosamides and streptogramins type B (the so-called MLSB phenotype). Other mechanisms of resistance involving cell impermeability or drug inactivation have been detected in Staphylococcus spp. and Escherichia coli. These strains are resistant to 14-membered macrolides (erythromycin and oleandomycin) but remain susceptible to spiramycin.
Generation of 5-fs pulses and octave-spanning spectra directly from a Ti:sapphire laser (2001)

Spectra extending from 600 to 1200 nm have been generated from a Kerr-lens mode-locked Ti:sapphire laser producing 5-fs pulses. Specially designed double-chirped mirror pairs provide broadband controlled dispersion, and a second intracavity focus in a glass plate provides additional spectral broadening. These spectra are to our knowledge the broadest ever generated directly from a laser oscillator.
A Purchase Protocol with Multichannel Authentication (2009)

While online shopping are becoming more accepted by people in modern life, cardholders are more concerned about card fraud and the lack of cardholder authentication in the current online credit card payment. This paper proposes a purchase protocol with live cardholder authentication for online transactions which combines telephone banking and online banking together. The order information and payment information are sent though the Internet and encrypted by asymmetric key encryption. The cardholder is authenticated by the card issuing bank ringing back at the customer’s phone number and the cardholder inputting the secure PIN and the amount to pay. The multichannel authentication makes the cardholder feel secure and card fraud difficult. Furthermore, the protocol does not require the cardholder to obtain public key certificates or install additional software for online transactions.
Clofazimine as elective treatment for granulomatous cheilitis. (2005)

Cheilitis granulomatosa (CG) is a rare, idiopathic inflammatory disorder that usually affects young adults and clinically is characterized by diffuse, non-tender, soft to firm swelling of one or both lips. A variant of granulomatous cheilitis is Melkersson-Rosenthal syndrome when associated with facial paralysis and furrowed tongue. Several treatments have been used with variable success. We report 3 cases of GC treated with oral clofazimine 100 to 200 mg daily for 3 to 6 months obtaining regression of lesions in all treated cases. Hyperpigmentation and elevation of liver enzymes were observed as side effects.
Annealed twists for stable tuned fused polished couplers (1993)

The fused polished coupler can be twist tuned in an oxybutane flame. The twist causes decoupling as the modal fields redistribute away from the coupler axis. The final structure is as stable as an untwisted fused polished coupler and is free from any torsional stress.
Analysis of the Pseudomonas putida CA-3 proteome during growth on styrene under nitrogen-limiting and non-limiting conditions (2009)

Pseudomonas putida CA-3 is a styrene-degrading bacterium capable of accumulating medium-chain-length polyhydroxyalkanoate (mclPHA) when exposed to limiting concentrations of a nitrogen source in the growth medium. Using shotgun proteomics we analysed global proteome expression in P. putida CA-3 supplied with styrene as the sole carbon and energy source under N-limiting (condition permissive for mclPHA synthesis) and non-limiting (condition non-permissive for mclPHA accumulation) growth conditions in order to provide insight into the molecular response of P. putida CA-3 to limitation of nitrogen when grown on styrene. A total of 1761 proteins were identified with high confidence and the detected proteins could be assigned to functional groups including styrene degradation, energy, nucleotide metabolism, protein synthesis, transport, stress response and motility. Proteins involved in the upper and lower styrene degradation pathway were expressed throughout the 48 h growth period under both nitrogen limitation and excess. Proteins involved in polyhydroxyalkanoate (PHA) biosynthesis, nitrogen assimilation and amino acid transport, and outer membrane proteins were upregulated under nitrogen limitation. PHA accumulation and biosynthesis were only expressed under nitrogen limitation. Nitrogen assimilation proteins were detected on average at twofold higher amounts under nitrogen limitation. Expression of the branched-chain amino acid ABC transporter was up to 16-fold higher under nitrogen-limiting conditions. Branched chain amino acid uptake by nitrogen-limited cultures was also higher than that by non-limited cultures. Outer membrane lipoproteins were expressed at twofold higher levels under nitrogen limitation. This was confirmed by Western blotting (immunochemical detection) of cells grown under nitrogen limitation. Our study provides the first global description of protein expression changes during growth of any organism on styrene and accumulating mclPHA (nitrogen-limited growth).
Spatial Audio Coding without Recourse to Background Signal Compression (2019)

The MPEG-H 3D audio standard applies singular value decomposition (SVD) to the input higher order ambisonics data, then encodes each predominant (foreground) sound component independently using a standard core audio codec. The residual (background) signal is encoded in the ambisonic domain. This paper is motivated by the observations: i) separate coding of SVD components ignores spatial inter channel masking effects; ii) compression in both SVD and ambisonic domains is difficult to perceptually optimize; iii) Only few predominant components are encoded due to the prohibitive side information cost of specifying SVD basis vectors. The proposed coding architecture overcomes the first two concerns by performing all compression in the SVD domain with a masking threshold that is calculated jointly for all encoded components, thereby accounting for cross-component masking. The third shortcoming is circumvented by a novel method for extending a given set of SVD basis vectors at no side information cost, by computing (at both encoder and decoder) basis vectors to span the null space of the transmitted basis vectors. Experimental results provide evidence for substantial objective and subjective gains.
Construction of Functional Coatings with Durable and Broad-Spectrum Antibacterial Potential Based on Mussel-Inspired Dendritic Polyglycerol and in Situ-Formed Copper Nanoparticles (2017)

A novel surface coating with durable broad-spectrum antibacterial ability was prepared based on mussel-inspired dendritic polyglycerol (MI-dPG) embedded with copper nanoparticles (Cu NPs). The functional surface coating is fabricated via a facile dip-coating process followed by in situ reduction of copper ions with a MI-dPG coating to introduce Cu NPs into the coating matrix. This coating has been demonstrated to possess efficient long-term antibacterial properties against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and kanamycin-resistant E. coli through an "attract-kill-release" strategy. The synergistic antibacterial activity of the coating was shown by the combination of two functions of the contact killing, reactive oxygen species production and Cu ions released from the coating. Furthermore, this coating inhibited biofilm formation and showed good compatibility to eukaryotic cells. Thus, this newly developed Cu NP-incorporated MI-dPG surface coating may find potential application in the design of antimicrobial coating, such as implantable devices.
Effect of Various Concentrations of Brilliant Green and Bile Salts on Salmonellae and Other Microorganisms (1965)

A study of the inhibitory effect of 24 different combinations of brilliant green and bile salts concentrations was conducted, using seven species of microorganisms capable of fermenting mannitol. The inhibitory effect of brilliant green decreased as the bile salts concentration was increased. Staphylococcus aureus and Proteus rettgeri were inhibited by all test media. Escherichia coli was inhibited in all but two combinations of brilliant green and bile salts. Aerobacter aerogenes generally followed a pattern of growth similar to that of three species of salmonellae. Three of the 24 combinations of brilliant green and bile salts showed little or no inhibition of salmonellae but did inhibit the other organisms studied.
The Size Effects on High Frequency Magnetic Properties of Amorphous Wires (1991)

The frequency dependence of the permeability, jU^, in amorphous Co-Fe- Si-B wires was investigated as a function of the wire diameter, annealing temperature, tensile stress and drawing parameters. Results show that the level of μ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i</sub> and the critical frequency for holding μ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i</sub> constant depend mainly on the wire diameter, suggesting that eddy currents affect μ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i</sub> at high frequencies. It was also found that two-stage annealing, in which drawing followed by annealing was performed twice, results in an improved frequency characteristic of μ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i</sub>
An Energy-Efficient IR-UWB Receiver Based on Distributed Pulse Correlator (2013)

A new circuit technique called distributed pulse correlator (DPC) is proposed for pulse detection in ultra-wideband impulse radio (IR-UWB) receivers. Among several IR-UWB transceiver architectures, an analog correlation receiver has the advantages of good performance and reduced circuit complexity, but requires a correlator and a template pulse with good time resolution. To achieve both fine time resolution and low power consumption, a DPC time-interleaves multiple sampling stages operating in a power-saving pulsed mode and incorporates a built-in mechanism to generate the reconfigurable local template pulse. The operation of the DPC is theoretically analyzed and its performance evaluated, followed by a detailed discussion of its circuit implementation. A chip prototype of a 3-10-GHz analog correlation receiver employing an eight-tap, 10-GSample/s DPC was designed and fabricated in a 0.18-μm standard digital CMOS technology. In the measurement, the DPC achieves a pulse rate of 250 MHz with an energy efficiency of 40 pJ/pulse, and the whole receiver achieves an energy efficiency of 190 pJ/pulse at the 250-MHz pulse rate. The complete IR-UWB link (a transmitter, a receiver, and antennas) is also tested.
[A comparative study on effect of two bisbenzylisoquinolines, tetrandrine and berbamine, on reversal of multidrug resistance]. (1997)

A comparative study on the effect of two bisbenzylisoquinolines, tetrandrine (TTD) and berbamine (BBM), and verapamil (VRP) on reversing multidrug resistance was reported. TTD, BBM and VRP showed significant activity in reversing adriamycin (ADR) and vincristine (VCR) resistance in acquired resistant MCF-7/Adr and KBv200 cell lines, and the effect was shown to be dose-dependent. TTD, at the concentration of 10 mumol.L-1, completely reversed ADR resistance in MCF-7/adr cells. TTD, BBM and VRP increased intracellular ADR accumulation in MCF-7/adr cells. There is minor difference in structure between TTD and BBM. TTD showed greater activity than VRP in reversing MDR, while BBM showed similar activity to that of VRP. TTD also showed significant activity in vivo in reversing ADR resistance in MDR MCF-7/Adr solid tumor in nude mice.