gebhart/patrepeval huggingface dataset:
On the Mechanism of Hydrogen Storage in a Metal−Organic Framework Material (2007)

Monte Carlo simulations were performed modeling hydrogen sorption in a recently synthesized metal-organic framework material (MOF) that exhibits large molecular hydrogen uptake capacity. The MOF is remarkable because at 78 K and 1.0 atm it sorbs hydrogen at a density near that of liquid hydrogen (at 20 K and 1.0 atm) when considering H2 density in the pores. Unlike most other MOFs that have been investigated for hydrogen storage, it has a highly ionic framework and many relatively small channels. The simulations demonstrate that it is both of these physical characteristics that lead to relatively strong hydrogen interactions in the MOF and ultimately large hydrogen uptake. Microscopically, hydrogen interacts with the MOF via three principle attractive potential energy contributions: Van der Waals, charge-quadrupole, and induction. Previous simulations of hydrogen storage in MOFs and other materials have not focused on the role of polarization effects, but they are demonstrated here to be the dominant contribution to hydrogen physisorption. Indeed, polarization interactions in the MOF lead to two distinct populations of dipolar hydrogen that are identified from the simulations that should be experimentally discernible using, for example, Raman spectroscopy. Since polarization interactions are significantly enhanced by the presence of a charged framework with narrow pores, MOFs are excellent hydrogen storage candidates.
Psychoanalysis and Literature: Past and Present (1993)

Click to increase image sizeClick to decrease image size Additional informationNotes on contributorsNorman N. HollandNorman N. Holland, Ph.D., Marston-Milbauer Eminent Scholar at the University of Florida.
CD3‐mediated apoptosis of human medullary thymocytes and activated peripheral T cells: Respective roles of interleukin‐1, interleukin‐2, interferon‐γ and accessory cells (1993)

Clonal deletion represents an important mechanism for the establishment of tolerance, by the elimination of autoreactive T cells. Deletion is accomplished by programmed cell death, termed apoptosis, induced by mobilization of the T cell receptor (TCR) on both thymocytes and mature T cells. The mechanism which drives T cells towards cell death or cell proliferation after TCR mobilization remains unclear. We show here that the mobilization of the CD3/TCR complex of both CD4+ and CD8+ single-positive medullary human thymocytes and human mature activated T cells, in the absence of accessory cells, leads to an activation-induced cell death process by apoptosis. In both cases, apoptosis was associated with interferon (IFN)-gamma gene expression and secretion in the absence of interleukin (IL)-2 gene expression; and the addition of anti-IFN-gamma antibody prevented cell death. Apoptosis could also be prevented by cyclosporin A (CsA) treatment and could be re-induced by the addition of IFN-gamma to CsA-treated cells. Addition of IL-2 had two different effects, it prevented apoptosis and also allowed proliferation in response to CD3 monoclonal antibody. Addition of IL-1, which induces IL-2 gene expression and secretion or addition of accessory cells, had the same preventive effect. These results suggest that the uncoupling of IFN-gamma and IL-2 gene expression following CD3/TCR mobilization initiates apoptosis of human T cells at several different stages during development and activation. We propose that co-signals provided by accessory cells allow a coupling of IL-2 gene and IFN-gamma gene expression, and that an essential role for IL-2 secretion in T cell activation involves the inhibition of a death program induced by IFN-gamma secretion.
Optimal conditions for freezing CHO‐S and HEK293‐EBNA cell lines: Influence of Me<sub>2</sub>SO, freeze density, and PEI‐mediated transfection on revitalization and growth of cells, and expression of recombinant protein (2008)

To avoid the time consuming, labor intensive seed-train expansion and to improve production reliability and consistency, portions of bulk cryopreserved cells from the same cultivation can be utilized as inocula or alternatively may be used to undertake transient transfections for large-scale bioreactor production. In this study, the conditions for large-scale freezing in cryobags were optimized utilizing a design of experiment approach. We showed that relatively high density of 30-40 x 10(6) cells/mL and relatively low Me(2)SO concentrations of 5-6% in the freezing media are optimal to freeze HEK293-EBNA and CHO-S cells in a controlled manner in order to achieve high viable cell recovery and growth post-thawing. The immediate transfer of freshly thawed cells into culture medium resulted in better cell growth compared to cells that were centrifuged in order to remove Me(2)SO. This was the case as long as the residual Me(2)SO did not exceed 0.2-0.3%. The best time to perform transient 25 kDa polyethylenimine-mediated transfection of pCEP4-EGFP plasmid into freshly thawed, one-step inoculated cells is after 72-96 h in culture. At this time point, the numbers of EGFP-positive cells in the freshly thawed culture mimic perfectly that of cells grown continuously. Finally, our data showed that it is possible to freeze transiently polyethyleneimine-transfected HEK293-EBNA cells and maintain growth rate and expression of recombinant protein following thawing. The optimal time point for freezing cells was 4 h after transfection.
An Improved Procedure for Generation of Half-Tone Computer Graphics Presentations (1969)

ability to half-tone with variable positions illumination sources.The workers were the author and K. Kelley and the drones were D
Stand-alone system for high-resolution, real-time terahertz imaging (2012)

In this work we present a stand-alone, portable system for high resolution real-time THz imaging. The total weight of the apparatus is less than 15 kg and its physical dimension is of ~(65 cm)3. A quantum cascade laser emitting at 3.4 THz laser based on a third-order distributed feedback cavity is used as source. It operates in continuous-wave at 50 K with more than 1 mW output power and less than 300 mW of power consumption. High resolution real-time THz imaging is reported: resolution of 2.5 times the wavelength is demonstrated.
Expression of a prostate-associated protein, human glandular kallikrein (hK2), in breast tumours and in normal breast secretions (2000)

The recent demonstration of human glandular kallikrein (hK2) expression in a breast carcinoma cell line has suggested that this putatively prostate-restricted, steroid hormone-regulated protease may also be expressed in breast epithelium in vivo and secreted into the mammary duct system. Given that the only substrate yet identified for hK2 activity is the precursor of prostate-specific antigen (PSA), the expression of which in breast carcinomas may be associated with favourable prognosis, our purpose was to examine the expression pattern of both hK2 and PSA in breast tumour tissues. Cytosolic extracts of 336 primary breast carcinomas prepared for routine oestrogen receptor (ER) and progesterone receptor (PR) analysis, as well as 31 nipple aspirates from six women with non-diseased mammary glands, were assayed for hK2 and PSA using immunofluorometric assays developed by the authors. In the tumour extracts, measurable hK2 and PSA concentrations were detected in 53% and 73% of cases respectively, and were positively correlated to each other (r = 0.59, P = 0.0001). Higher concentrations of PSA and hK2 were found in tumours expressing steroid hormone receptors (P = 0.0001 for PSA and P = 0.0001 for hK2, by Wilcoxon tests for both ER and PR), and both PSA (r = 0.25, P = 0.0001) and hK2 (r = 0.22, P = 0.0001) correlated directly with PR levels. A negative correlation between patient age and PSA (r = -0.12, P = 0.03) was also found. Both proteins were present in nipple aspirate fluid at relatively high concentrations which were positively correlated (r = 0.53, P = 0.002). The molecular weights of the immunoreactive species quantified by the hK2 and PSA assays were established by high-performance liquid chromatography (HPLC) and were consistent with the known molecular weights of hK2 and PSA. Together these data provide the first evidence, to our knowledge, that both malignant breast tissue and normal breast secretion contain measurable quantities of hK2, and that the degree of hK2 expression or secretion is directly proportional to the expression of PSA and steroid hormone receptors. hK2 expression may therefore be a marker of steroid hormone action in breast tissue.
Partially depleted SOI NMOSFET's with self-aligned polysilicon gate formed on the recessed channel region (1997)

A new SOI NMOSFET with a "LOCOS-like" shape self-aligned polysilicon gate formed on the recessed channel region has been fabricated by a mix-and-match technology. For the first time, we developed a new scheme for implementing self-alignment in both source/drain and gate structure in recessed channel device fabrication. Symmetric source/drain doping profile was obtained and highly symmetric electrical characteristics were observed. Drain current measured from 0.3 μm SOI devices with V/sub z/ of 0.773 V and T/sub ox/=7.6 nm is 360 μA/μm at V/sub GS/=3.5 V and V/sub DS/=2.5 V. Improved breakdown characteristics were obtained and the BV/sub DSS/ (the drain voltage for 1 nA/μm of I/sub D/ at T/sub GS/=0 V) of the device with L/sub eff/=0.3 μm under the floating body condition was as high as 3.7 V.
Anycast-RP Using Protocol Independent Multicast (PIM) (2006)

This specification allows Anycast-RP (Rendezvous Point) to be used inside a domain that runs Protocol Independent Multicast (PIM) only. Other multicast protocols (such as Multicast Source Discovery Protocol (MSDP), which has been used traditionally to solve this problem) are not required to support Anycast-RP. This memo extends the Register mechanism in PIM so Anycast-RP functionality can be retained without using MSDP.
H18F + Sb2O3: a new selective radiofluorinating agent (1986)

18F was selectively introduced into trihalomethyl groups using H18F and Sb2O3. E.g., treatment of PhCClF2 with H18F and Sb2O3 at 220° for 20 min gave 44% PhC18FF2.
The antiviral activity of dipyridamole. (1977)

Dipyridamole, a coronary vasodilatator, was found to possess antiviral activity against representatives of different families. The antiviral properties were studied in chick embryo, human diplid and FL cell cultures by the agar diffusion plaque inhibition and plaque reduction tests and one-step growth cycle experiments. The inhibition of the virus-induced cytopathic effect was estimated quantitatively. Dipyridamole significantly inhibited the yield of members of the viral families Picornaviridae, Togaviridae, Orthomyxoviridae, Paramyxoviridae, Herpetoviridae and Poxviridae, as well as of Chlamydiaceae (sheep abortion agent).
Contextual Distance for Data Perception (2007)

Structural perception of data plays a fundamental role in pattern analysis and machine learning. In this paper, we develop a new structural perception of data based on local contexts. We first identify the contextual set of a point by finding its nearest neighbors. Then the contextual distance between the point and one of its neighbors is defined by the difference between their contribution to the integrity of the geometric structure of the contextual set, which is depicted by a structural descriptor. The centroid and the coding length are introduced as the examples of descriptors of the contextual set. Furthermore, a directed graph (digraph) is built to model the asymmetry of perception. The edges of the digraph are weighted based on the contextual distances. Thus direction is brought to the undirected data. And the structural perception of data can be performed by mining the properties of the digraph. We also present the method for deriving the global digraph Laplacian from the alignment of the local digraph Laplacians. Experimental results on clustering and ranking of toy problems and real data show the superiority of asymmetric perception.
Fabrication of a Glass‐Ceramic‐to‐Metal Seal Between Ti–6Al–4V and a Strontium Boroaluminate Glass (2016)

Glass‐ceramics are widely utilized in the electronics industry to provide electrical insulation and to form leak‐tight joints with a range of metals. The coefficient of thermal expansion ( CTE ) of the glass‐ceramic can be controlled by the extent of crystallization to reduce detrimental tensile stresses in the joint. In recent years, there has been interest in using titanium alloys, in place of stainless steels, due to their lower density and superior specific strength. In this study, the heat treatment of a strontium boroaluminate glass has been tailored to create glass‐ceramics with mean CTE s ranging from 5.7 ± 0.1 × 10 −6 /K to 9.7 ± 0.1 × 10 −6 /K over the temperature range 303–693 K. The resultant glass‐ceramic consists of three crystalline phases and residual glass. A glass‐ceramic with a mean CTE of 6.9 ± 0.1 × 10 −6 /K was subsequently fabricated to form a compression seal with a Ti–6Al–4V housing and a preoxidized Kovar pin. Single pin assemblies were shown to be reproducible in terms of microstructure and all passed a standard helium leak test, indicating that a successful seal had been produced.
Nuclide Composition Benchmark Data Set for Verifying Burnup Codes on Spent Light Water Reactor Fuels (2002)

To establish a nuclide composition benchmark data set for the verification of burnup codes, destructive analyses of light water reactor spent-fuel samples, which were cut out from several heights of spent-fuel rods, were carried out at the analytical laboratory at the Japan Atomic Energy Research Institute. The 16 samples from three kinds of pressurized water reactor (PWR) fuel rods and the 18 samples from two boiling water reactor (BWR) fuel rods were examined. Their initial 235U enrichments and burnups were from 2.6 to 4.1% and from 4 to 50 GWd/t, respectively. One PWR fuel rod and one BWR fuel rod contained gadolinia as a burnable poison. The measurements for more than 40 nuclides of uranium, transuranium, and fission product elements were performed by destructive analysis using mass spectrometry, and alpha-ray and gamma-ray spectrometry. Burnup for each sample was determined by the 148Nd method. The analytical methods and the results as well as the related irradiation condition data are compiled as a complete benchmark data set.
Nonsteroidal anti-inflammatory drug gastropathy. We started it--can we stop it? (1986)

We first documented the disturbing frequency of serious gastropathy associated with nonsteroidal anti-inflammatory drug (NSAID) use in a series of 140 patients with rheumatoid arthritis more than a decade ago.<sup>1</sup>Now there is increasing evidence from more accurate endoscopic studies that supports our concern. Reports of the Food and Drug Administration to the Arthritis Advisory Committee indicate that there are increasing national and foreign data regarding the high frequency of gastrointestinal tract and peptic ulcer complications resulting from NSAID use. The reports also indicate that gastrointestinal tract bleeding is reported twice as often as ulcers. Since more than 27 million prescriptions for NSAIDs were written in the past year alone, and more than 14 million patients with arthritis are known to take NSAIDs regularly, the public health implications are evident. A recent multicenter hospital study determined that 1.8 billion dollars in rheumatoid arthritis hospital care costs reflected gastropathy complications
STATUS OF THE 100 MEV PREINJECTOR FOR THE ALBA (2008)

An obstructed inguinal hernia is a common surgical emergency, which presents with a variety of contents like the small intestine, omentum, and colon. Intestinal knotting is a rare entity encountered in surgical practice; it occurs when one coil of intestine wraps around the other and eventually leads to complications such as intestinal obstruction, ischemia, and gangrene. Both conditions are considered surgical emergencies and should be dealt with through appropriate surgical measures forthwith. We report the case of an obstructed inguinal hernia, which, on exploration, showed an ileoileal knot as its content. Ileoileal knotting is a very rare phenomenon and, to the best of our knowledge, such an ileoileal knot as a content of obstructed inguinal hernia has not been reported in the surgical literature so far.
Effect of deoxycholic acid on Ca<sup>2+</sup>movement, cell viability and apoptosis in human gastric cancer cells (2014)

Deoxycholic acid (DOA) is one of the secondary bile acids used as a mild detergent for the isolation of membrane associated proteins. This study examined whether the secondary bile acid, DOA, altered Ca(2+) movement, cell viability and apoptosis in SCM1 human gastric cancer cells. The Ca(2+)-sensitive fluorescent dye fura-2 was used to measure [Ca(2+)]i. DOA-evoked [Ca(2+)]i rises concentration dependently. The response was reduced by removing extracellular Ca(2+). DOA-evoked Ca(2+) entry was inhibited by store-operated Ca(2+) channel inhibitors (nifedipine, econazole and SKF96365), the protein kinase C (PKC) activator phorbol 12-myristate 13 acetate (PMA) and the PKC inhibitor GF109203X. In Ca(2+)-free medium, treatment with the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (TG) abolished DOA-evoked [Ca(2+)]i rises. Conversely, treatment with DOA abolished TG-evoked [Ca(2+)]i rises. Inhibition of phospholipase C with U73122 abolished DOA-evoked [Ca(2+)]i rises. At 100-500 μM, DOA decreased cell viability, which was not changed by chelating cytosolic Ca(2+) with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA/AM). DOA between 100 and 300 μM also induced apoptosis. Collectively, in SCM1 cells, DOA-induced [Ca(2+)]i rises by evoking phospholipase C-dependent Ca(2+) release from the endoplasmic reticulum and Ca(2+) entry via store-operated Ca(2+) channels. DOA also caused Ca(2+)-independent apoptosis.
Welding robots (2003)

Using robots in industrial welding operations is common but far from being a streamlined technological process. The problems are with the robots, still in their early design stages and difficult to use and program by regular operators; the welding process, which is complex and not really well known and the human-machine interfaces, which are unnatural and not really working. In this article, these problems are discussed, and a system designed with the double objective of serving R&D efforts on welding applications and to assist industrial partners working with welding setups is presented. The use of object-oriented and distributed software to assist industrial robotic welding applications is discussed. The system is explained in some detail and demonstrated using two test cases that reproduce two situations common in industry: multilayer butt welding and multipoint fillet welding.
Risk factors and raised atherosclerotic lesions in coronary and cerebral arteries. Statistical analysis from the Oslo study. (1981)

In 1972-1973, about 16,200 men living in Oslo, aged 40 to 49 years, were examined for cardiovascular disease, and had a number of coronary risk factors measured. This report gives the results of 129 autopsied cases with regard to the association between raised atherosclerotic lesions in coronary and cerebral arteries and various coronary risk factors. For coronary raised lesions, the high density lipoprotein (HDL) cholesterol ratio was the most significant risk factor. Systolic blood pressure and total serum cholesterol were also significantly associated. Physical activity at work and at leisure, nonfasting triglycerides, and cigarette smoking did not show a significant association with coronary artery raised lesions. The association between total serum cholesterol and systolic blood indicates that total serum cholesterol may be more important than systolic pressure in the synergism affecting the development of coronary atherosclerosis. For cerebral artery raised lesions, blood pressure was the most important risk factor, even though serum cholesterol was highly associated with the lesions. The interaction analysis also suggested that blood pressure was more important than serum cholesterol in the synergism.
An empirical investigation of program spectra (1998)

A variety of expensive software maintenance and testing tasks require a comparison of the behaviors of program versions. Program spectra have recently been proposed as a heuristic for use in performing such comparisons. To assess the potential usefulness of spectra in this context, we conducted an experiment that examined the relationship between program spectra and program behavior, and empirically compared several types of spectra. This paper reports the results of that experiment.
Identification of inhibitors of SARS-CoV-2 in-vitro cellular toxicity in human (Caco-2) cells using a large scale drug repurposing collection (2020)

<title>Abstract</title> To identify possible candidates for progression towards clinical studies against SARS-CoV-2, we screened a well-defined collection of 5632 compounds including 3488 compounds which have undergone clinical investigations (marketed drugs, phases 1 -3, and withdrawn) across 600 indications. Compounds were screened for their inhibition of viral induced cytotoxicity using the human epithelial colorectal adenocarcinoma cell line Caco-2 and a SARS-CoV-2 isolate. The primary screen of 5632 compounds gave 271 hits. A total of 64 compounds with IC50 <20 µM were identified, including 19 compounds with IC50 < 1 µM. Of this confirmed hit population, 90% have not yet been previously reported as active against SARS-CoV-2 in-vitro cell assays. Some 37 of the actives are launched drugs, 19 are in phases 1-3 and 10 pre-clinical. Several inhibitors were associated with modulation of host pathways including kinase signaling P53 activation, ubiquitin pathways and PDE activity modulation, with long chain acyl transferases were effective viral inhibitors.
Pathological roles of the VEGF/SphK pathway in Niemann–Pick type C neurons (2014)

Sphingosine is a major storage compound in Niemann-Pick type C disease (NP-C), although the pathological role(s) of this accumulation have not been fully characterized. Here we found that sphingosine kinase (SphK) activity is reduced in NP-C patient fibroblasts and NP-C mouse Purkinje neurons (PNs) due to defective vascular endothelial growth factor (VEGF) levels. Sphingosine accumulation due to inactivation of VEGF/SphK pathway led to PNs loss via inhibition of autophagosome-lysosome fusion in NP-C mice. VEGF activates SphK by binding to VEGFR2, resulting in decreased sphingosine storage as well as improved PNs survival and clinical outcomes in NP-C cells and mice. We also show that induced pluripotent stem cell (iPSC)-derived human NP-C neurons are generated and the abnormalities caused by VEGF/SphK inactivity in these cells are corrected by replenishment of VEGF. Overall, these results reveal a pathogenic mechanism in NP-C neurons where defective SphK activity is due to impaired VEGF levels.