gebhart/patrepeval huggingface dataset:
Beneficial actions of exogenous hyaluronic acid on wound healing. (1991)

To determine the effect of exogenous hyaluronic acid (HA) on healing of experimental wounds, responses in the hamster cheek pouch were measured after a hole was cut through the tissue with a biopsy punch. Fluorescence-labeled dextran was administered intravenously as a macromolecular tracer and the microcirculation was observed in vivo with a fluorescence microscope connected to a high-resolution television system. In one group a gelatin sponge soaked in 1.5 ml 16 mg/dl HA in water was applied topically at the time of injury and on postinjury days 1, 3, 5, and 7. The control group received the sponge soaked in the aqueous vehicle. Every 2 days after injury, the microcirculation was observed or histologic specimens were harvested. Wound size decreased almost twice as fast with HA compared with its vehicle (p less than 0.05). Healing was defined as time for total wound closure with at least one microvessel bridging the site of injury and required 16 or more days with vehicle but averaged less than 9 days with HA. Early during healing the repair site was surrounded by widespread extravasation of the fluorescent tracer, an index of inflammation; this area was reduced by two thirds 2 to 4 days after injury with HA compared with its vehicle (p less than 0.05). The density of perfused microvessels was twofold higher with HA 2 to 4 days after injury (p less than 0.05). However, microvessel density was similar in both groups by 6 days after injury and remained similar for at least 45 days after injury, which suggests that HA evoked no unusual angiogenic response. Histologic examination of fixed, stained specimens showed increases in intravascular leukocytes after injury and treatment-related differences in the distribution of intravascular leukocytes in 20 to 40 microns and 40 to 80 microns diameter microvessels 1 to 2 days after injury. Otherwise, leukocyte infiltration during healing was similar in both groups. The mechanism for the beneficial action of HA on healing is unknown. However, several in vitro studies suggest that HA is part of a feedback loop that promotes cell proliferation and migration in actively growing tissues. Alternatively, the role of HA in water homeostasis could favor tissue hydration, which has a well-known beneficial effect on healing.
Systematic Characteristic Exploration of the Chimeras Generated in Multiple Displacement Amplification through Next Generation Sequencing Data Reanalysis (2015)

This study exhibited the profile of the phi29MDA chimeras by tens of millions of chimeric sequences, and helped understand the amplification mechanism of the phi29 DNA polymerase. Our work also illustrated the importance of NGS data reanalysis, not only for the improvement of data utilization efficiency, but also for more potential genomic information.
Alkaline Plating Baths Containing Organic Amines: Part II . Copper Plating from Solutions Containing Ethylenediamine (1938)

Several solutions were prepared, containing copper sulfate and ethylenediamine, from which bright and firmly adherent deposits of copper were obtained on iron at temperatures of from 20° C. to 65° C. and current densities ranging from 0.025 amp./sq. in. (0.4 amp./dm. 2 ) to 0.20 amp./sq. in. (3.2 amp./dm. 2 )
All-optical helicity dependent magnetic switching in an artificial zero moment magnet (2014)

Low remanent magnetization as key prerequisite for the ability of helicity dependent all-optical magnetic switching (AOS) is demonstrated for an artificial zero moment magnet. A heterostructure consisting of two amorphous ferrimagnetic Tb36Fe64 and Tb19Fe81 alloy layers is designed to yield a zero remanent net magnetization at room temperature by means of an antiparallel interfacial exchange coupling of the dominant magnetic moments. The canceling layer magnetizations provide vanishing demagnetization fields and the ability of AOS. Contrary to this, no all-optical switching is observed for single Tb36Fe64 and Tb19Fe81 films. This study provides further evidence that the ability for all-optical magnetic switching is correlated to the remanent sample magnetization and thus to the difference in magnetic moment of the rare-earth and transition-metal sublattices.
Zur Biochemie der Schilddrüsenfunktion VII; Anzeichen der tierexperimentellen E-Hypervitaminose (1947)

Abstract Vitamin-E-reiche Fütterung von männlichen jungen Kaninchen führt nach mehreren Wochen zu krankhaften Erscheinungen an der Schilddrüse (SD). Die drei Symptome der entstandenen E-Hypervitaminose in dem innersekretorischen Organ sind: 1. Abnorme Volumen- und Gewichtszunahme, 2. Jodfreiheit bzw. -mangel und 3. histologische Veränderung (gesteigertes Epithelwachstum). Vitamin-E-Gaben neutralisieren nicht die schilddrüsenschädigende Wirkung von Kohlkropfnahrung. Ein täglicher α-Toko-pherol-Zusatz zum Normalfutter verhindert nicht den kropferzeugenden Thiouracil-Effekt an Kaninchen, sondern wirkt im gleichen Sinne.
Prime Ricerche sulla Fisionomia Enzimatica Caratteristica della Fotosintesi (1954)

Summary The rate of oxidation of glucose-6-phosphate, ribose-5-phosphate, fructose-1,6- phosphate, iso-citrate and malate in extracts from green and etiolated pea leaves was determined, using the triphenyltetrazolium technique. Glucose-6-phosphate, ribose-5-phosphate, and fructose-1,6-phosphate, in the presence of added TPN, where oxidized at a rate about twice higher in the extracts from green than in the extracts from etiolated leaves. Iso-citrate, in the presence of TPN, fructose-1,6-phosphate, in the presence of DPN, and malate, in the presence of either TPN or DPN, were oxidized at about the same rate in the two types of extracts. These data seem to indicate a preferential synthesis of enzymes involved in the metabolic cycle of phosphorylated sugars during the transition of the leaf from the etiolated to the photosynthetising physiognomy. They seem also favourable to the view assigning to this metabolic system a primary importance in the anabolic pathway of photosynthesis.
Isorhamnetin Ameliorates Dry Eye Disease via CFTR Activation in Mice (2021)

Dry eye disease is one of the most common diseases, with increasing prevalence in many countries, but treatment options are limited. Cystic fibrosis transmembrane conductance regulator (CFTR) is a major ion channel that facilitates fluid secretion in ocular surface epithelium and is a potential target of therapeutic agent for the treatment of dry eye disease. In this study, we performed a cell-based, high-throughput screening for the identification of novel natural products that activate CFTR and restore the aqueous deficiency in dry eye. Screening of 1000 natural products revealed isorhamnetin, a flavonol aglycone, as a novel CFTR activator. Electrophysiological studies showed that isorhamnetin significantly increased CFTR chloride current, both wild type and ∆F508-CFTR. Isorhamnetin did not alter intracellular cAMP levels and the activity of other ion channels, including ANO1, ENaC, and hERG. Notably, application of isorhamnetin on mouse ocular surface induced CFTR activation and increased tear volume. In addition, isorhamnetin significantly reduced ocular surface damage and expression of interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α in an experimental mouse model of dry eye. These data suggest that isorhamnetin may be used to treat dry eye disease.
Frequencies of ALK and ROS in NSCLC FFPE tumor samples utilizing a highly specific immunohistochemistry-based assay and FISH analysis. (2010)

10536 Background: Receptor tyrosine kinases ALK and ROS are expressed as oncogenic fusion proteins in approximately 2%-5% of all non-small cell lung cancer (NSCLC) patients. Most recently, ALK targeted therapies have been shown to be effective in ALK positive patients. This may also be true for ROS but is less validated. The ability to identify patients that harbor these fusion proteins is desirable. Currently, molecular assays such as fluorescence in situ hybridization (FISH) and RT-PCR are used to identify these patients. We reasoned that an immunohistochemistry (IHC)-based assay could provide a fast, inexpensive alternative for diagnosing patients with ALK and ROS abnormalities. We have developed an IHC-based assay for the detection of low level ALK and ROS in NSCLC patients with high specificity and sensitivity. Methods: We generated highly specific rabbit monoclonal antibodies against ALK and ROS. ALK and ROS antibodies were evaluated in a panel of 656 NSCLC FFPE tumor tissues. The majority of tissues (n = 409) were analyzed in tissue microarray format while the remaining (n = 247) were whole tissue sections. IHC positive tissues were also analyzed by FISH to compare protein expression to the presence of ALK and ROS rearrangements. Results: Western blot, IHC and FISH experiments confirm that our monoclonal antibodies can specifically detect low levels of both ROS and ALK in NSCLC tumor samples. Approximately, 4.1% (27 cases) of NSCLC cases analyzed were ALK positive by IHC. We were able to confirm these results by FISH in 25/27 cases. The remaining 2 cases we were unable to score due to poor tissue quality. We identified 2.6% (17/656) of the NSCLC tissues to be ROS positive by IHC. We confirmed these results in 16/17 tissues by FISH analysis. Due to technical issues, we were unable to score the remaining tissue. Both the ALK and ROS antibodies are highly specific. We are currently performing additional studies to determine sensitivity. ALK and ROS were never observed in the same tumor sample. Conclusions: We conclude that IHC staining with ALK and ROS monoclonal antibodies is a highly specific assay that reliably identifies aberrant ALK and ROS protein expression in NSCLC patients. No significant financial relationships to disclose.
Run-Time Services for Hybrid CPU/FPGA Systems on Chip (2006)

Modern FPGA devices, which include (multiple) processor core(s) as diffused IP on the silicon die, provide an excellent platform for developing custom multiprocessor systems-on-programmable chip (MPSoPC) architectures. As researchers are investigating new methods for migrating portions of applications into custom hardware circuits, it is also critical to develop new run-time service frameworks to support these capabilities. Hthreads (HybridThreads) is a multithreaded RTOS kernel for hybrid FPGA/CPU systems designed to meet this new growing need. A key capability of hthreads is the migration of thread management, synchronization primitives, and run-time scheduling services for both hardware and software threads into hardware. This paper describes the hthreads scheduler, a key component for controlling both software-resident threads (SW threads) and threads implemented in programmable logic (HW threads). Run-time analysis shows that the hthreads scheduler module helps in reducing unwanted system overhead and jitter when compared to historical software schedulers, while fielding scheduling requests from both hardware and software threads in parallel with application execution. Run time analysis shows the scheduler achieves constant time scheduling for up to 256 active threads with a total of 128 different priority levels, while using uniform APIs for threads requesting OS services from either side of the hardware/software boundary
Chemical synthesis of a human fibroblast interferon gene and its expression in Escherichia coli. (1983)

Using the solid phase phosphotriester method, a gene coding for human fibroblast interferon consisting of 166 amino acid residues was chemically synthesized. The gene obtained by ligation of 61 synthesized oligodeoxyribonucleotide fragments was inserted into the downstream of tryptophan promoter, and expressed in E. coli. Lysate of this E. coli showed the antiviral activity which was specifically neutralized by anti-fibroblast interferon antibody. No particular advantage was observed in the expression efficiency by the synthetic gene over that by the native gene.
A Convenient Approach Towards the Conversion of H-Phosphonate and H-Phosphonothioate Diesters into Phosphoro(di)thioate Derivatives (1990)

S-Benzyl and S-phenyl thiosuccinimides (N-benzylthio-and N-phenylthiosuccinimides) react, in the presence of the base N,N-diisopropylethylamine (DIPEA), with phosphonate and phosphonothioate diesters (HP(O)(OR)2 and HP(S)(OR)2) to give the corresponding S-benzyl(phenyl) phosphorothioates or phosphorodithioates, respectively. Further, S,S-dibenzyl-(diphenyl) phosphorodithioates can be prepared by the reaction of a hydroxylic component with the reactive species generated in situ from ammonium phosphinate, the earlier mentioned individual thiosuccinimides and the activating reagent 2,4,6-triisopropylbenzenesulfonyl chloride.
Origami-Inspired Approaches for Biomedical Applications (2020)

Modern day biomedical applications require progressions that combine advanced technology with the conformability of naturally occurring, complex biosystems. These advancements yield conformational interactions between the biomedical devices and the biological organisms' structures. Biomedical applications that adapt origami-inspired approaches have accrued aspired advances. Along with application-specific advantages, the most pertinent advances provided by origami-inspired strategies include voluminous structures with the ability to conform to biosystems, shape-shifting from two-dimensional (2D) to three-dimensional (3D) structures, and biocompatibility. Throughout this paper, the exploration of new studies, primarily within the past decade, with origami-based applications of biomedical devices, including their theories, experimental results, and plans for future testing are reviewed. This mini-review contains examples that aid the advancement of biomedical applications and hold promising future discoveries. The origami-inspired applications discussed within this paper are tissue scaffolds, drug delivery approaches, stents and catheters, implants, microfluidic devices, biosensors, and origami usage in surgery.
Fuzzy optimal control for double inverted pendulum (2012)

In this paper a fuzzy optimal control for stabilizing an upright position a double inverted pendulum (DIP) is developed and compared. Modeling is based on Euler-Lagrange equations. This results in a complicated nonlinear fast reaction, unstable multivariable system. Firstly, the mathematical models of double pendulum system are presented. The weight variable fuzzy input is gained by combining the fuzzy control theory with the optimal control theory. Simulation results show that the controller, which the upper pendulum is considered as main control variable, has high accuracy, quick convergence speed and higher precision.
The structure and organization of a proline-rich protein gene of a mouse multigene family. (1985)

One gene of the mouse proline-rich protein multigene family was cloned on a 3.6-kilobase pair EcoRI/BglII DNA fragment from a (partial) Sau3A bacteriophage library of CD-1 mouse chromosomal DNA. Phage harboring the gene were identified by plaque hybridization using 32P-labeled proline-rich protein cDNA inserts from clones pRP33 and pMP1 obtained from rat and mouse, respectively. The transcriptional unit includes three exonic sequences separated by 1434 base pairs (intron I) and 450 base pairs (intron II). The complete primary structure of the gene and the 5' and 3' flanking regions (3595 base pairs) were determined by the Maxam and Gilbert (Maxam, A.M., and Gilbert, W. (1980) Methods Enzymol. 65, 499-560) sequencing method. The DNA on the 5' side of exon I contains several sequences that may be involved in the induction and expression of this mouse gene. These sequences include putative regulatory sites such as those considered to be inducible by cAMP and steroids, Z-DNA and enhancer sequences and the expected TATAA and CAAT boxes. The mature protein coding region, exon II, is not interrupted with intron sequences. Exon III is located in the nontranslated region and contains the poly(A) addition site. The deduced amino acid sequence showed that the protein encoded by this gene contains 13 tandemly repeat regions, each 14 amino acids in length, with the prototype sequence PPPPGGPQPRPPQG. Each amino acid within the repeat has a favored codon. The consensus DNA sequence for each repeat is CCA CCA CCA CCA GGA GGC CCA CAG CCG AGA CCC CCT CAA GGC. The high degree of conservation of both nucleotide and amino acid sequences within the repeat region suggests that proline-rich protein genes likely evolved by gene duplication of a 42-base pair internal repeat.
Tobacco Mosaic Virus Delivery of Phenanthriplatin for Cancer therapy (2016)

Phenanthriplatin, cis-[Pt(NH3)2Cl(phenanthridine)](NO3), is a cationic monofunctional DNA-binding platinum(II) anticancer drug candidate with unusual potency and cellular response profiles. Its in vivo efficacy has not yet been demonstrated, highlighting the need for a delivery system. Here we report tobacco mosaic virus (TMV) as a delivery system for phenanthriplatin. TMV forms hollow nanotubes with a polyanionic interior surface; capitalizing on this native structure, we developed a one-step phenanthriplatin loading protocol. Phenanthriplatin release from the carrier is induced in acidic environments. This delivery system, designated PhenPt-TMV, exhibits matched efficacy in a cancer cell panel compared to free phenanthriplatin. In vivo tumor delivery and efficacy were confirmed by using a mouse model of triple negative breast cancer. Tumors treated with PhenPt-TMV were 4× smaller than tumors treated with free phenanthriplatin or cisplatin, owing to increased accumulation of phenanthriplatin within the tumor tissue. The biology-derived TMV delivery system may facilitate translation of phenanthriplatin into the clinic.
Systematic design of program analysis frameworks (1979)

Semantic analysis of programs is essential in optimizing compilers and program verification systems. It encompasses data flow analysis, data type determination, generation of approximate invariant assertions, etc.
Study of Two-Dimensional Magnetic Recording System Including Micromagnetic Writer (2014)

Micromagnetic writing on 8 nm grains and readback with various readers have been studied. For a conventional writer recording a pseudorandom binary sequence with 2 Tb/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> channel bit density, user densities of 1.52 and 1.09 Tb/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> could be achieved with a rotated single sense head (RSH) and a normally oriented single head (NSH), respectively, using 2 nm sampling period, a minimum mean squared error equalizer, and a Viterbi detector. Simulation indicated that an RSH with multiple scans and 2-D equalization provided better resistance to a skew angle of 15° than an NSH. An optimized shingled writer was proposed; simulation indicated that an RSH and rotated head array could reach a user areal density of 3.76 and 4.52 Tb/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for two grains per channel bit, which was close to the predicted maximum user areal density (4.66 Tb/in <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> ) for this grain size obtained with an ideal writer and reader.
Review of Kachru (1982/1983): The Other Tongue: English across Cultures (1983)

Preview this article: Braj B. Kachru, ed., The Other Tongue: English across Cultures, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/eww.4.1.13pla-1.gif
Simple Colorimetric Detection of Amyloid <i>β</i>‐peptide (1–40) based on Aggregation of Gold Nanoparticles in the Presence of Copper Ions (2015)

A simple method for specific colorimetric sensing of Alzheimer's disease related amyloid-β peptide (Aβ) is developed based on the aggregation of gold nanoparticles in the presence of copper ion. The detection of limit for Aβ(1-40) is 0.6 nM and the promising results from practical samples (human serum) indicate the great potential for the routine detection.
Enhancement of Learning Achievement of Organic Chemistry Using Inquiry-based Semi-small Scale Experiments (SSSEs) (2014)

This study aimed to investigate students’ learning achievement and retention of organic chemistry by using seven inquiry-based Semi-Small Scale Experiments (SSSEs) of 21 hours duration. These experiments included: 1) functional groups, 2) hydrocarbon compounds, 3) alcohols and ethers, 4) aldehydes and ketones, 5) carboxylic acids, 6) esters, and 7) amines and amides. Fifty-one Grade 12 students at Khukhan School in Srisaket province of Thailand during the first academic semester of 2012 were purposively selected as participants. The data collected, included students’ learning achievement test scores of organic chemistry, learning retention scores 14 days after the implementation, and a survey of student satisfaction in learning inquiry-based SSSEs, were analyzed. The paired-samples t-test analysis indicated that the students obtained an average post-test score of learning achievement (mean 29.53, SD 2.98) statistically higher than that of the average pre-test score (mean 13.76, SD 1.98) at 0.05 level of significance. It also showed that the students’ average learning retention score (mean 28.82, SD 3.05) was not different from their post-test score of achievement at 0.05 level of significance. In addition, their satisfaction in learning inquiry-based SSSEs was at a ‘high’ level (mean 4.14, SD 0.33). It can be implied that the inquiry-based SSSEs were effective in the promotion of students’ learning as the students obtained high scores in the learning achievement and retention tests and were highly satisfied with the SSSEs.
Dynamic puddle delineation and modeling of puddle-to-puddle filling-spilling-merging-splitting overland flow processes (2013)

[1] Surface microtopography affects overland flow, infiltration, soil erosion, pollutant transport, and other fundamental hydrologic and environmental processes across scales. Under the influence of surface depressions, overland flow essentially features a series of progressive puddle-to-puddle (P2P) filling, spilling, merging, and splitting processes. The objectives of this study are to characterize puddles and their hierarchical relationships and model the microtopography-controlled P2P processes. We proposed a new modeling framework for simulating the P2P overland flow dynamics through cell-to-cell (C2C) and P2P routing for a set of puddle-based units (PBUs) in a well-delineated, cascaded P2P drainage system. Testing of the P2P model demonstrated its potential to improve overland flow modeling and hydrologic connectivity analysis by explicitly incorporating the hydrologic roles of depressions and quantifying the real microtopography-controlled P2P dynamics.