gebhart/patrepeval huggingface dataset:
Engineering a “Methionine Clamp” into Src Family Kinases Enhances Specificity toward Unnatural ATP Analogues (2003)

A single alanine or glycine mutation in the ATP binding site of a protein kinase allows unique use of an unnatural analogue of ATP (N(6)-(benzyl) ATP) as a phosphodonor, which is not accepted by wild-type kinases. Addition of [gamma(32)P] N(6)-(benzyl) ATP to a cell lysate containing an ATP analog-specific kinase allele (as1 allele) results in the exclusive radiolabeling of bona fide substrates of the mutant kinase. Here we report efforts to engineer kinase alleles that have enhanced selectivity for ATP analogues and decreased catalytic activity with ATP, thus increasing the signal-to-noise ratio of substrate labeling. Two conserved leucine residues that contact each face of the adenine ring of ATP were mutated to methionine. The introduction of this "methionine clamp" resulted in Src and Fyn kinase alleles that have markedly improved specificity for unnatural N(6)-substituted ATP analogues over the natural substrate, ATP. This preference for unnatural nucleotides is reflected in more efficient labeling of protein substrates in cell extracts using the new analogue-specific v-Src allele. Kinase alleles with enhanced selectivity for unnatural ATP analogues should greatly facilitate the ultimate goal of labeling kinase substrates in intact cells, where concentrations of ATP and other competing nucleotides are high.
Isolation of the gene encoding the Saccharomyces cerevisiae centromere-binding protein CP1. (1990)

CP1 is a sequence-specific DNA-binding protein of the yeast Saccharomyces cerevisiae which recognizes the highly conserved DNA element I (CDEI) of yeast centromeres. We cloned and sequenced the gene encoding CP1. The gene codes for a protein of molecular weight 39,400. When expressed in Escherichia coli, the CP1 gene directed the synthesis of a CDEI-binding protein having the same gel mobility as purified yeast CP1. We have given the CP1 gene the genetic designation CEP1 (centromere protein 1). CEP1 was mapped and found to reside on chromosome X, 2.0 centimorgans from SUP4. Strains were constructed in which most of CEP1 was deleted. Such strains lacked detectable CP1 activity and were viable; however, CEP1 gene disruption resulted in a 35% increase in cell doubling time and a ninefold increase in the rate of mitotic chromosome loss. An unexpected consequence of CP1 gene disruption was methionine auxotrophy genetically linked to cep1. This result and the recent finding that CDEI sites in the MET25 promoter are required to activate transcription (D. Thomas, H. Cherest, and Y. Surdin-Kerjan, J. Mol. Biol. 9:3292-3298, 1989) suggest that CP1 is both a kinetochore protein and a transcription factor.
Crystal-trim and its application to investigations on channeling effects during ion implantation (1994)

Abstract The formalism for the binary collision code Crystal-TRIM which simulates ion implantation effects in crystalline solids is presented. The treatment of binary collisions, the kinematics and the crystal description are elucidated in detail. The nuclear scattering is described by the ‘universal’ potential. The scattering angles are calculated by semianalytical and analytical formulae. We employ a semiempirical model to describe the electronic stopping of the ions. Thermal vibrations of lattice atoms, the radiation-induced production of vacancies and interstitials and their accumulation, the generation of static lattice disorder due to electronic energy deposition and the influence of a thin amorphous surface layer are considered in the simulation model. The program Crystal-TRIM is applied to calculate range profiles for P+ and B+ implantation into Si at energies between 500 keV and 1 MeV, at ion incidence into axial and planar channels and into ‘random’ directions, and at ion doses between 1013 and 1014 cm−2. A relatively good agreement with experimental data is found if the effects of radiation damage, static lattice disorder and the influence of an amorphous surface layer are taken into account simultaneously. We study dechanneling processes during ion implantation. The dechanneled fraction of the ion beam is calculated in dependence on the depth. Furthermore, we illustrate the motion of individual projectiles within the crystalline solid.
Global Incidence of Multidrug‐Resistant Tuberculosis (2006)

These updated sets of estimates incorporating previously treated TB cases call for an urgent plan to expand appropriate diagnostic and treatment services for patients with MDR-TB in low-resource settings.
Field emission characteristics of CuO nanowires grown on brown-oxide-coated Cu films on Si substrates by conductive heating in air (2006)

CuO nanowires were grown on brown-oxide-coated Cu films on Si substrates by conductive heating in air and their field emission characteristics were investigated. High resolution scanning electron microscopy showed that the CuO nanowires grew perpendicularly to the substrate, and their length and radius could be controlled by varying heating temperature and time. It was found that the lengths of the CuO nanowires were saturated to a certain point with respect to the heating temperature, and the increasing heating time increased the uniformity of the CuO nanowires in length. The field emission characteristics were strongly affected by the formation of the brown-oxide layer and the morphologies of the CuO nanowires governed by heating temperature and time.
Magnetite recovery in coal washing by high gradient magnetic separation. Final report (1977)

The report describes a demonstration of the successful recovery of magnetite from mixtures of magnetite and coal, like those found in a coal-washing circuit, by High Gradient Magnetic Separation. The demonstration was part of a research program at Francis Bitter National Magnet Laboratory. High values of magnetite recovery were achieved at reasonably high material throughput rates with little coal found reporting to the magnetics. A single-stage separator incorporating a new matrix design was used at rates up to 4.4 tons of solids per hour per square foot of matrix cross section (300 gpm/sq ft). At this troughput rate, more than 99% of the magnetite was trapped along with less than 5% of the coal. Magnetic field values no higher than 6 kilogauss were used to achieve these results, a value well within the range of present commerical magnet designs.
Optimizing CMOS implementations of the C-element (1997)

The C-element is one of the most frequently used primitives in asynchronous control circuits. This paper introduces various single-rail and double-rail implementations of the C-element, explains ways to optimize their performance and compares them with respect to energy and speed in a micropipeline control circuit environment. The issue of divergence of the complementary outputs in the double-rail implementations is also discussed.
Alternating cerebral hemispheric activity and the lateralization of autonomic nervous function. (1983)

Alternating dominance of cerebral hemispheric activity was demonstrated in humans by use of the electroencephalogram (EEG). Relative changes of electrocortical activity have a direct correlation with changes in the relative nostril dominance, the so-called nasal cycle. The nasal cycle is a phenomenon where efficiency of breathing alternates predominantly through right or left nostril with a periodicity ranging from 25 to greater than 200 minutes. Relatively greater integrated EEG value in one hemisphere correlates with predominant airflow in the contralateral nostril, defining a new interrelationship between cerebral dominance and peripheral autonomic nervous function.
PointINS: Point-Based Instance Segmentation (2021)

In this paper, we explore the mask representation in instance segmentation with Point-of-Interest (PoI) features. Differentiating multiple potential instances within a single PoI feature is challenging, because learning a high-dimensional mask feature for each instance using vanilla convolution demands a heavy computing burden. To address this challenge, we propose an instance-aware convolution. It decomposes this mask representation learning task into two tractable modules as instance-aware weights and instance-agnostic features. The former is to parametrize convolution for producing mask features corresponding to different instances, improving mask learning efficiency by avoiding employing several independent convolutions. Meanwhile, the latter serves as mask templates in a single point. Together, instance-aware mask features are computed by convolving the template with dynamic weights, used for the mask prediction. Along with instance-aware convolution, we propose PointINS, a simple and practical instance segmentation approach, building upon dense one-stage detectors. Through extensive experiments, we evaluated the effectiveness of our framework built upon RetinaNet and FCOS. PointINS in ResNet101 backbone achieves a 38.3 mask mean average precision (mAP) on COCO dataset, outperforming existing point-based methods by a large margin. It gives a comparable performance to the region-based Mask R-CNN K. He, G. Gkioxari, P. Dollár, and R. Girshick, "Mask R-CNN," in Proc. IEEE Int. Conf. Comput. Vis., 2017, pp. 2980-2988 with faster inference.
Diffusion-weighted Imaging Improves the Diagnostic Accuracy of Conventional 3.0-T Breast MR Imaging (2010)

DW imaging with glandular tissue-normalized ADC assessment improves the characterization of breast lesions beyond the characterization achieved with conventional 3D T1-weighted and dynamic contrast-enhanced MR imaging at 3.0 T.
A regulatory CD9<sup>+</sup>B-cell subset inhibits HDM-induced allergic airway inflammation (2015)

This finding strengthens the potential for Breg-targeted therapies in allergic asthma.
A high performance RF power amplifier with protection against load mismatches (2003)

A high performance power amplifier is presented which includes a protection circuitry against load impedance variations. Load mismatches produce peak voltages on the power transistor collector that give rise to breakdown conditions and hence to permanent faults. The protection circuitry is based on a feedback loop that acts on the amplifier gain to limit the overdrive of the output transistor. A monolithic power amplifier for 1.8-GHz DCS-PCS applications was integrated in a silicon bipolar technology with a 4.3-V breakdown voltage. The amplifier delivers a 33-dBm output power with 51% power-added efficiency and 33-dB gain at a nominal 3-V supply voltage. Standing wave ratio was tested up to 20:1 for all phases with a supply voltage up to 3.8 V without damage to the device.
Parallel Parsing Algorithms and VLSI Implementations for Syntactic Pattern Recognition (1984)

Earley's algorithm has been commonly used for the parsing of general context-free languages and the error-correcting parsing in syntactic pattern recognition. The time complexity for parsing is 0(n3). This paper presents a parallel Earley's recognition algorithm in terms of an ``X*'' operator. By restricting the input context-free grammar to be ¿-free, the parallel algorithm can be executed on a triangular-shape VLSI array. This array system has an efficient way of moving data to the right place at the right time. Simulation results show that this system can recognize a string with length n in 2n + 1 system time. We also present a parallel parse-extraction algorithm, a complete parsing algorithm, and an error-correcting recognition algorithm. The parallel complete parsing algorithm has been simulated on a processor array which is similar to the triangular VLSI array. For an input string of length n the processor array will give the correct right-parse at system time 2n + 1 if the string is accepted. The error-correcting recognition algorithm has also been simulated on a triangular VLSI array. This array recognizes an erroneous string of length n in time 2n + 1 and gives the correct error count. These parallel algorithms are especially useful for syntactic pattern recognition.
New methodology in iodonium salt synthesis. Reactions of [hydroxy(tosyloxy)iodo]arenes with aryltrimethylsilanes (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew methodology in iodonium salt synthesis. Reactions of [hydroxy(tosyloxy)iodo]arenes with aryltrimethylsilanesGerald F. Koser, Richard H. Wettach, and Carol S. SmithCite this: J. Org. Chem. 1980, 45, 8, 1543–1544Publication Date (Print):April 1, 1980Publication History Published online1 May 2002Published inissue 1 April 1980https://pubs.acs.org/doi/10.1021/jo01296a050https://doi.org/10.1021/jo01296a050research-articleACS PublicationsRequest reuse permissionsArticle Views1094Altmetric-Citations80LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Innovations in microwave filters and multiplexing networks for communications satellite systems (1992)

The evolution of microwave filters and multiplexers for space application is described. The many advances are described within the context of the design of the overall communication subsystem. Emphasis is placed on the multipaction and passive intermodulation (PIM) considerations in designing high-power multiplexers. The impact of microwave filter technology in the channel characterization of satellite systems is described. The future directions of research and development are briefly discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Development of a Gas–Liquid Two-Phase Flow Sensor Applicable to CBM Wellbore Annulus (2016)

The measurement of wellbore annulus gas-liquid two-phase flow in CBM (coalbed methane) wells is of great significance for reasonably developing gas drainage and extraction processes, estimating CBM output, judging the operating conditions of CBM wells and analyzing stratum conditions. Hence, a specially designed sensor is urgently needed for real-time measurement of gas-liquid two-phase flow in CBM wellbore annulus. Existing flow sensors fail to meet the requirements of the operating conditions of CBM wellbore annulus due to such factors as an inapplicable measurement principle, larger size, poor sealability, high installation accuracy, and higher requirements for fluid media. Therefore, based on the principle of a target flowmeter, this paper designs a new two-phase flow sensor that can identify and automatically calibrate different flow patterns of two-phase flows. Upon the successful development of the new flow sensor, lab and field tests were carried out, and the results show that the newly designed sensor, with a measurement accuracy of ±2.5%, can adapt to the operating conditions of CBM wells and is reliable for long-term work.
Detection and estimation of oestrogenic constituents in red clover (1962)

Abstract A two‐dimensional paper chromatographic method has been used for the separation and detection of all the known oestrogenic isoflavones and coumestrol in red clover. The presence of other isoflavone components in red clover is suggested. The concentrations of the individual oestrogenic constituents were determined by spectrophotometric measurement after separation by paper chromatography. Biochanin A has been found to be the predominant oestrogen in red clover, while formononetin (the other major isoflavone) is inactive.
Influence Of Deubiquitinating Enzymes On Mutagenesis In Saccharomyces Cerevisiae (2011)

In recent years, it became clear that the mutagenic effect of base alterations in a DNA template is dependent on bypass synthesis, carried out by one or more translesion polymerases. A critical role for proliferating cell nuclear antigen (PCNA) and its ubiquitination following DNA damage has been established. Among Saccharomyces cerevisiae deletion mutants in which UV mutagenesis is compromised, we identified and characterized a mutant of the ubiquitin recycling protein Doa4. Similar cases may be represented by Doa1, previously described by others, as well as Bro1 and Ubi4. We discuss overall altered ubiquitin levels or failure to deubiquitinate specific target proteins as likely explanations. This study is of relevance for understanding and possibly modifying the mutagenic effect of DNA-damaging agents in environmental toxicology, cancer treatment and cancer prevention.
Architectural acoustics—Hear here (2016)

The discipline of Architectural Acoustics consistently produces more than 100 papers across six or more special sessions, at each meeting of the ASA. Student paper awards, student design competitions, and Knudsen lectures augment these activities. Joint sessions, particularly with noise, musical acoustics, psychological and physiological acoustics, and signal processing in acoustics, add more still to the architectural acoustics goings-on at every ASA conference. The sphere of influence is not limited to ASA alone, as TCAA members participate in the Green Construction Code of the International Code Council, Society of Motion Picture and Television Engineers Study Group: Movie Theater Sound System Measurement and Adjustment Techniques, Classroom Acoustics Standards, the American Institute of Architects Continuing Education System, and more. This busy committee also produces a steady stream of publications documenting recent work and deciphering standards for key stakeholders. Anyone with an interest in the field will find many opportunities to advance their own expertise, build a network of colleagues, friends and mentors, and contribute to the essential activities of the Technical Committee on Architectural Acoustics.
Graphene: preparation and structural perfection (2010)

Three basic processes for the production of monolayer graphene are reviewed: cleavage of graphite crystals, exfoliation of graphite intercalation compounds and chemical vapor deposition. The relationship of the structural perfection of the obtained thin flakes to the preparation method is discussed.
Hybridization histochemical and immunohistochemical localization of inhibin/activin subunits and messenger ribonucleic acids in the rat brain (1996)

Inhibin and activin are best known as gonadal glycoprotein hormones but have a broad anatomical distribution. We previously described the central distribution ofinhibin/activin beta A- and beta B-subunit proteins in some neuronal cell bodies, fibers, and nuclei of the rat brain and reported a possible role for central activin in suckling-induced oxytocin secretion and corticotropin releasing factor release. In the present report, we mapped the detailed immunohistochemical localization of inhibin/activin alpha-, beta A-, and beta B-subunits throughout the rat brain to further clarify their central distribution. In addition, the localization and distribution of their corresponding mRNAs was assessed. The results are summarized as follows: 1) Both beta A- and beta B-subunit immunoreactivity are found in neuronal cell bodies in the nucleus of the solitary tract and the dorsal and ventral medullary reticular nuclei, and in fibers and terminals of known projection sites for these nuclei. 2) beta B-subunit immunoreactivity is localized in a group of perifornical neurons in the hypothalamus. 3) beta A-subunit immunoreactivity is present in discrete populations of neuronal cell nuclei scattered throughout the CNS. 4) mRNAs encoding each of the inhibin/activin subunits are expressed in all major brain regions as determined by S1 nuclease assay and in a variety of specific neuroanatomical sites as shown by in situ hybridization. The results suggest that central inhibin and activin proteins are produced in the brain where they may potentially serve inter- and intracellular functions in multiple systems.
Ischemia and reperfusion-induced arrhythmias in the rat. Effects of xanthine oxidase inhibition with allopurinol. (1984)

We have investigated the possibility that xanthine oxidase-linked free radical production has a role in the genesis of arrhythmias during ischemia and reperfusion. In this study, rats were treated with allopurinol (20 mg/kg, orally, 24 hours before study, plus 20 mg/kg, iv, 15 minutes prior to study). Using an anesthetized open-chest preparation with either coronary artery occlusion for 30 minutes, or 5 minutes followed by 10 minutes reperfusion, we monitored and compared the rhythm disturbances in experimental vs. placebo-treated rats (n = 18 in each group). Allopurinol treatment reduced the incidence of ventricular tachycardia during ischemia from 88% to 50% (P less than 0.05) and the number of premature ventricular complexes from 471 +/- 120 to 116 +/- 46 (P less than 0.02), but the treatment had no effect upon the incidence or duration of ventricular fibrillation or upon mortality. In contrast, far more dramatic protection was observed during reperfusion after 5 minutes of ischemia. Allopurinol treatment reduced the incidence of ventricular fibrillation from 67% to 11% (P less than 0.01), reduced the mean duration of fibrillation from 230 +/- 70 to 14 +/- 1 seconds (P less than 0.05), and reduced mortality by half (10/18 to 4/18), although this did not reach a level of statistical significance. In addition, the mean duration of tachycardia was reduced from 83 +/- 26 to 38 +/- 8 seconds (P less than 0.05). Allopurinol pretreatment thus affords some protection against ischemia-induced arrhythmias, but a higher degree of protection against reperfusion-induced arrhythmias. Allopurinol inhibits xanthine oxidase activity, and, in turn, this inhibits superoxide radical production.(ABSTRACT TRUNCATED AT 250 WORDS)
The Sensitivity, Specificity and Accuracy of Warning Signs in Predicting Severe Dengue, the Severe Dengue Prevalence and Its Associated Factors (2018)

<i>Objectives:</i> To study Malaysian dengue clinical practice guideline (CPG) warning signs (WS) in predicting severe dengue (SD) and its associated factors among confirmed cases presented to a teaching hospital in north-eastern Malaysia in 2014. <i>Methods:</i> A cross-sectional study was performed in February 2015 using secondary data acquired from the hospital records. There were 2607 confirmed dengue cases presented to Hospital Universiti Sains Malaysia (HUSM) in 2014. Seven hundred patients were selected after proportionate stratified random sampling conducted according to the number of cases in 12 different months in 2014. Data were collected and analysed using SPSS version 22.0. <i>Results:</i> Severe dengue outcomes represented 4.9% of cases. The prevalence of any of WS in SD was 91.2%. The most common WSs prior to SD were persistent vomiting (55.9%), and abdominal pain/tenderness (52.9%). The most sensitive warning sign in detecting SD was abdominal pain (59%). Specificity of individual WS were generally good, especially of clinical fluid accumulation (99%), hepatomegaly (98%) and mucosal bleeding (93%). Factors associated with SD were persistent vomiting (Adjusted odds ratio (aOR)): 2.41), mucosal bleeding (aOR: 4.73) and haematocrit rise with rapid platelet drop (aOR: 2.74). <i>Conclusion:</i> A focus on sensitivity, specificity, predictive values and association of a number of particular WS should be emphasized in order to better predict severe dengue outcomes.