gebhart/patrepeval huggingface dataset:
Affinity Chromatography of<i>Pseudomonas</i>Isoamylase on Cross-linked Amylose Gel (1977)

Journal Article Affinity Chromatography of Pseudomonas Isoamylase on Cross-linked Amylose Gel Get access Kanji Kato, Kanji Kato The Institute of Scientific and Industrial Research, Osaka University, Yamada-kami, Suita-shi, Osaka 565, Japan Search for other works by this author on: Oxford Academic Google Scholar Yoshio Konishi, Yoshio Konishi The Institute of Scientific and Industrial Research, Osaka University, Yamada-kami, Suita-shi, Osaka 565, Japan Search for other works by this author on: Oxford Academic Google Scholar Akinori Amemura, Akinori Amemura The Institute of Scientific and Industrial Research, Osaka University, Yamada-kami, Suita-shi, Osaka 565, Japan Search for other works by this author on: Oxford Academic Google Scholar Tokuya Harada Tokuya Harada The Institute of Scientific and Industrial Research, Osaka University, Yamada-kami, Suita-shi, Osaka 565, Japan To whom reprint should be sent. Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 41, Issue 10, 1 October 1977, Pages 2077–2080, https://doi.org/10.1080/00021369.1977.10862809 Published: 01 October 1977 Article history Received: 08 June 1977 Published: 01 October 1977
A Novel Mouse β-Defensin, mBD-6, Predominantly Expressed in Skeletal Muscle (2001)

Defensins comprise a family of cationic antimicrobial peptides that is characterized by the conserved 6 cysteine residues. They are expressed in the epithelial cells of various organs and are identified as key elements in the host defense system at the mucosal surface. We isolated a novel mouse beta-defensin gene from the bacterial artificial chromosome DNA containing the mouse beta-defensin-3 gene. The full-length cDNA was cloned from skeletal muscle cDNA and called mouse beta-defensin-6 (mBD-6). The predicted peptide conserved the 6-cysteine motif and had 59% amino acid sequence identity with mouse beta-defensin-3 and 59% identity with mouse beta-defensin-4. We demonstrated the expression of mBD-6 in skeletal muscle in addition to the esophagus, tongue, and trachea. In animal models of endotoxemia, mBD-6 expression was also induced in the lung. mBD-6 showed potent antimicrobial activity against Escherichia coli and would play an important role in host defense in the esophagus, airways, and skeletal muscle. mBD-6 is the first reported beta-defensin predominantly expressed in skeletal muscle. This unique tissue specificity suggests some novel physiological roles of this peptide family.
Calmodulin Regulates Fodrin Susceptibility to Cleavage by Calciumdependent Protease I (1989)

The intracellular calcium-dependent proteolysis of fodrin has been postulated to be central to the regulation of plasticity of the cortical cytoskeleton of many eukaryotic cells. The close proximity of the sites of calmodulin (CaM) binding and calcium-dependent protease I (CDP-I) cleavage in mammalian alpha-fodrin suggested that their action may be linked. In hypotonic and isotonic buffers, CDP-I proteolysis of the beta subunit of fodrin was absolutely dependent upon the presence of active CaM. The stimulation by CaM was inhibited by CaM antagonists. The rate of CDP-I proteolysis of both subunits was enhanced by CaM, while the rate of fodrin proteolysis with other proteases was not influenced by CaM. The increase in the susceptibility of fodrin to CDP-I proteolysis was half-maximal at 80 nM CaM, and maximal at 200 nM CaM. The unusual and differential susceptibility of alpha- and beta-fodrin to proteolysis by CDP-I in the absence of CaM was exploited to investigate the quaternary structure of fodrin in which only the alpha subunit was cleaved. Cleavage of the alpha subunit alone did not destroy the tetrameric form of the molecule, whereas CDP-I cleavage of both subunits rendered the molecule incapable of reforming tetramers. These results provide structural and functional evidence that CaM and CDP-I act synergistically in the regulated proteolysis of fodrin.
Application of the bispectrum to glottal pulse analysis (2003)

Higher order spectral (HOS) techniques, such as the bispectrum, offer robustness to Gaussian noise and the ability to recover phase information. However, their drawbacks, such as the high variance of estimates and the need for long data records, have limited their use in conventional speech processing applications. As in glottal pulse estimation, all existing inverse filtering approaches use second-order statistics, it is of interest to explore the potential of HOS in this area. Using the theory of HOS factorization and the linear bispectrum, it is shown how voiced speech can be modelled as a nonGaussian coloured noise driven system. The linear bispectrum approach can be used to obtain alternative glottal pulse and vocal tract estimates in hybrid Iterative Adaptive Inverse Filtering (hIAIF) and the results are compared with traditional IAIF. Finally, a new technique which involves joint estimation of the glottal pulse and vocal tract followed by inverse filtering is presented. This new technique shows good preliminary results and is much simpler than previous techniques.
Transient Intermediates in the Laser Flash Photolysis of Ketoprofen in Aqueous Solutions:  Unusual Photochemistry for the Benzophenone Chromophore (1997)

The transient intermediates in the nanosecond laser flash photolysis of ketoprofen, an aryl propionic acid, show the formation of a carbanion in aqueous solutions at pH 7.1. This carbanion incorporates spectroscopic properties from both a ketyl radical anion and a benzylic radical. The ketoprofen carboxylate undergoes biphotonic photoionization, a process that contributes less than 10% to its photodecomposition and leads to a benzylic-type radical after decarboxylation with a rate constant ≥1 × 107 s-1. On the other hand, the carbanion forms monophotonically and the unsuccessful attempts to sensitize the formation of the ketoprofen triplet excited state in aqueous solutions suggest that the carbanion precursor is either an excited singlet state or an extremely short-lived triplet. In organic solvents of lower polarity, the excited triplet state is readily detectable.
Optimal Trajectory Control of Burst Mode for LLC Resonant Converter (2012)

The LLC resonant converter has drawn much attention for high efficiency. However, its light-load efficiency still cannot meet the ever increasing requirements. This paper proposes a novel burst-mode control for the LLC resonant converter to improve the light-load efficiency. Based on the state-plane trajectory analysis, during the burst-on time, a three-pulse switching pattern is implemented. By optimizing the first pulse width, the state variables are settled to the steady state of the highest efficiency load in the minimal time, and then, the next two pulse operation follows this steady-state trajectory to achieve high burst efficiency. Moreover, when the load changes, the burst-on time with the optimal three-pulse switching pattern is maintained, but the burst-off time is modulated. As a result, the output-voltage low-frequency ripple minimally keeps constant, and no additional filter is needed. Experimental results validate the proposed techniques.
Altered Monocyte Responses to Defined TLR Ligands in Patients With Primary Biliary Cirrhosis * (2005)

The role of the adaptive immune response, with regard to the development of autoantibodies, has been extensively studied in primary biliary cirrhosis (PBC). However, the importance of innate immunity has been noted only recently. Based on the proposed role of microorganisms in the pathogenesis of the disease, we hypothesize that patients with PBC possess a hyper-responsive innate immune system to pathogen-associated stimuli that may facilitate the loss of tolerance. To address this issue, we isolated peripheral blood monocytes from 33 patients with PBC and 26 age-matched healthy controls and stimulated such cells in vitro with defined ligands for toll-like receptor (TLR) 2 (lipoteichoic acid; LTA), TLR3 (polyIC), TLR4 (lipopolysaccharide; LPS), TLR5 (flagellin), and TLR9 (CpG-B). Supernatant fluids from the cultures were analyzed for levels of 5 different pro-inflammatory cytokines, interleukin (IL)-1beta, IL-6, IL-8, IL-12p70, and TNF-alpha. After in vitro challenge with TLR ligands, PBC monocytes produced higher relative levels of pro-inflammatory cytokines, particularly IL-1beta, IL-6, IL-8, and TNF-alpha, compared with controls. In conclusion, monocytes from patients with PBC appear more sensitive to signaling via select TLRs, resulting in secretion of selective pro-inflammatory cytokines integral to the inflammatory response that may be critical in the breakdown of self-tolerance.
In Vitro Inhibition of Lens Epithelial Cell Proliferation and Migration (1988)

Delayed opacification of the posterior capsule is the most common cause of decreased visual acuity after extracapsular cataract extraction. In humans, this has been shown to result from migration and possibly proliferation of residual lens epithelial cells onto the central posterior capsule. We studied a group of pharmacologic agents to determine their ability to inhibit lens epithelial cell proliferation and migration in vitro. A drug capable of inhibiting lens epithelial cell growth and/or migration, if free of unacceptable toxic effects on other cell populations, might be used to prevent lens capsule opacification. Anti-proliferative activity was exhibited by several agents, with 50% inhibition of growth occurring at the following concentrations: 5-fluorouracil, 30 micrograms/ml; daunomycin, 10 ng/ml; colchicine, 15 ng/ml; doxorubicin, 5 ng/ml; dexamethasone, 100 micrograms/ml; and cytosine arabinoside, 100 ng/ml. Colchicine inhibited lens epithelial cell migration by 50% at 20 ng/ml.
Physicochemical Characteristics of Self-Aggregates of Hydrophobically Modified Chitosans (1998)

Chitosan derivatives containing 2.8−5.1 deoxycholic acid groups per 100 anhydroglucosamine units of chitosan were synthesized by an EDC-mediated coupling reaction. Physicochemical properties of self-aggregates of the hydrophobically modified chitosans in aqueous media were studied by the dynamic light scattering method and fluorescence spectroscopy. The mean diameter (dH) of DC4.2 self-aggregates in phosphate-buffered saline (PBS) solution (pH 7.2) was 159 nm with unimodal size distribution (variance = 0.082). It seemed that the interparticle interaction between self-aggregates was almost negligible. The mean diameter decreased with increasing degree of substitution (DS), pH, or ionic strength of the medium. The critical aggregation concentration (cac) of self-aggregates was determined from the fluorescence emission spectra of pyrene. The cac value of DC4.2 in PBS solution (pH 7.2) was 2.6 × 10-2 mg/mL. The cac values decreased with increasing DS, pH, or ionic strength of the medium.
Characterization of a ferroelectric liquid crystal-based time delay unit for phased array antenna applications (1997)

An experimental demonstration of a photonic time delay unit (PTDU) based on ferroelectric liquid crystal devices and Thompson polarization beamsplitters (TBSs) is presented. High signal-to-noise ratios (SNRs) (>96 dB) and optical polarization extinction ratios (ER,/spl sim/40 dB) are demonstrated for the PTDU using an active noise filter technique. Using a 5 V peak square wave switch drive signal, a 70 /spl mu/s switching speed is demonstrated for this PTDU that can be used in fast (e.g., 5000 beam/s) beam scanning phased array systems. A shorter switching speed of 35 /spl mu/s is also observed using a high 15 V peak transitional voltage driving signal.
Whole cell yeast biotransformations in two-phase systems: Effect of solvent on product formation and cell structure (1992)

Biotransformation of benzaldehyde and pyruvate to (R)-phenylacetyl carbinol bySaccharomyces cerevisiae was investigated in two-phase aqueous-organic reaction media. With hexane as organic solvent, maximum biotransformation activity was observed with a moisture content of 10%. Of the organic solvents tested, highest biotransformation activities were observed with hexane and hexadecane, and lowest activities occurred with chloroform and toluene. Biocatalyst samples from biphasic media containing hexane, decane and toluene manifested no apparent cell structural damage when examined using scanning electron microscopy. In contrast, cellular biocatalyst recovered from two-phase systems containing chloroform, butylacetate and ethylacetate exhibited damage in the form of cell puncturing after different incubation periods. Phospholipids were detected in reaction media from biocatalytic systems which exhibited cell damage in electron micrographs. Phospholipid release was much lower in the two-phase systems containing toluene or hexane or in 100% aqueous biocatalytic system.
LA INTENCIONALIDAD EN LA FORMACIÓN DE VALORES PROFESIONALES: ¿MANIPULACIÓN O DESARROLLO? (2003)

RESUMEN Hoy dia, la formacion de valores en el contexto universitario cobra una importancia relevante si se tienen en cuenta, en primer lugar, los nuevos retos en el campo cientifico tecnologico y, en segundo lugar, la necesidad de un egresado competente, investido de conocimientos pero tambien de valores profesionales que lo distingan como ser humano. Este articulo presenta una reflexion acerca de las posibilidades que brinda la educacion universitaria para contribuir a la formacion de valores profesionales en el estudiante, desde la concepcion curricular hasta la intencion docente desde el aula. Se resalta el papel de la comunicacion educativa en el proceso de ensenanza aprendizaje y el acercamiento de la situacion aulica al desempeno profesional, mediante la ejecucion por parte del estudiante, de tareas semejantes a las profesionales, todo ello como una premisa para la contribucion a la formacion de la personalidad del educando. Se asumen concepciones contemporaneas acerca de los valores que consideran al estudiante como sujeto de su propia formacion, lo cual elimina la posibilidad de la manipulacion de la personalidad. Palabras claves : Valores profesionales, proceso de ensenanza aprendizaje, comunicacion educativa, manipulacion, personalidad. SUMMARY: Nowadays, the formation of human values in the university context receives an excellent importance if they consider, in the first place, the new challenges in the technological scientific field and, secondly, the necessity of competent withdrawing, invested with knowledge but also with professional values that distinguish it like being human. This paper presents a reflection about the possibilities that the university education offers to contribute to the formation of professional values in the student, from the curricular conception to the educational intention from the classroom. The paper of the educative communication in the process of education learning and the approach of the aulic situation to the professional performance is emphasized, by means of the execution by the student of similar tasks to the professionals, all it like a premise for the contribution to the formation of the personality of educating. Contemporary conceptions are assumed about the values that consider to the student like subject of their own formation, which eliminates the possibility of the manipulation of the personality. Key words : Professional values, process of education learning, educative communication, manipulation, personality.
An Analytical Performance Model for Partitioning Off-Chip Memory Bandwidth (2013)

With the emergence of multi-programmed workloads for Chip Multiprocessors (CMP), Quality of Service (QoS) of each co-scheduled application on the CMP is increasingly gaining importance. As more and more applications are consolidated into a single chip to compete for the limited off-chip memory bandwidth, off-chip memory bandwidth partitioning makes an increasing impact on system performance. Although various existing heuristic-based memory scheduling schemes have achieved significant system performance improvement by better partitioning the bandwidth, it is still not clear what are the best ways to partition off-chip bandwidth for improving different system performance objectives. The goal of this paper is to understand how off-chip memory bandwidth partitioning affects various system performance objectives. To achieve this goal, we propose an analytical model that is simple yet powerful enough to reveal the relationship between various memory bandwidth partitioning schemes and different system performance objectives. From our model, optimal memory bandwidth partitioning schemes for different system-level objectives are derived. Experimental results from a cycle-accurate full-system simulator show that, for heterogeneous workloads, performance improvements over No_partitioning/Equal_partitioning in terms of harmonic weighted speedup, minimum fairness, weighted speedup and sum of IPCs are 20.3%/2.1%, 49.8%/38.7%, 32.8%/7.6% and 64.2%/24%, on average, with our corresponding optimal partitioning schemes (i.e., Square_root, Proportional, Priority_APC, Priority_API), respectively.
Fast settling PLL frequency synthesizer utilizing the frequency detector method speedup circuit (1997)

We propose two items for the fast frequency settling in the phase locked loop (PLL) frequency synthesizer. One is a PLL frequency synthesizer utilizing a frequency detector method speedup circuit (FDMSC). From the experimental results, it is observed that fast frequency settling can be achieved. The other is a shortcut lowpass filter (LPF) method with the FDMSC in the PLL frequency synthesizer. The frequency settling time has been speeded up further by changing the time constant of the LPF to a smaller value only in the rising condition.
Preparation of activated fibrous carbon from phenolic fabric and its molecular sieving properties. (1987)

フェノール樹脂系繊維(カイノール)を出発原料として,N2,CO2,H2O,LPG燃焼ガスなどを用いて,おもに800～1000℃ で熱分解炭化および炭化工程を省略した直接賦活を行なって,表面積や細孔容積などの大きく異なる繊維状活性炭の製造を試みた。他方,通常の粒状活性炭についても生ヤシ殻からの比較製造実験を行ない,両者の細孔の開発挙動や生成活性炭の細孔構造特性や気相(水蒸気,ベンゼン,シクロヘキサン)と液相(ヨウ素)での吸着特性などを評価.対比した。得られたおもな結果は,つぎのとおりである。(1)カイノール繊維からは2000～3000m2/gの高表面積をもつ繊維状活性炭が収率10～30%で得られた。(2)吸着分離で重要な半径10nm以下のミクロ細孔容積については,いずれの原料からも約1.2cm3/gをもつ活性炭が得られたが,繊維状活性炭の細孔分布は半径0.8～1.Onmにのみ鋭利な中心ピークをもつ,ほぼ理想的な単峰型分布であった。(3)ヤシ殻活性炭では半径10nm以下の細孔領域でも単峰型分布でなく,半径10～104nmのマクロ細孔容積をも共存している点が繊維状活性炭と相違した。(4)原料と賦活度の異なる生成活性炭に対するベンゼンとシクロヘキサンの吸着特姓の比較から,カイノール繊維状活性炭の細孔入口形状がスリット状であることが検証された。また,ベンゼンとシクロヘキサンの分子最小径0.37とO.48nmの違いに基づく質量分離比16:1の優れた分子ふるい効果が確認され,製造条件の選定により,細孔径の制御が可能であることが確かめられた。(5)細孔物性や吸着特牲の比較基準として用いた,石炭原料の市販粒状活性炭は,多くの点でヤシ殻粒状活性炭と類似した特性を示した。
Langwidere: a new facial animation system (2002)

This paper presents Langwidere, a facial animation system that integrates hierarchical spline modeling with simulated muscles based upon local area surface deformations. The multi-level shape representation of a hierarchical spline provides control over the extent of deformations, while at the same time reducing the amount of data needed to define the surface. The facial model is constructed from a single closed surface defining the entire head and internal structures of the mouth cavity. Simulated muscles are attached to various levels of the surface with more rudimentary levels substituting for bone such as the skull and jaw. The combination of a hierarchical model and simulated muscles provides a smooth surface with precise and flexible control over surface shape.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Wideband Frequency-Domain Characterization of High-Impedance Probes (2001)

We investigated the broadband microwave properties of high-impedance probes designed for on-wafer waveform measurements. We show that the standard two-tier characterization method fails. We introduce two new methods of characterization, both of which yield equivalent results.
Formation of three‐dimensional fetal myocardial tissue cultures from rat for long‐term cultivation (2006)

Three-dimensional cardiomyocyte cultures offer new possibilities for the analysis of cardiac cell differentiation, spatial cellular arrangement, and time-specific gene expression in a tissue-like environment. We present a new method for generating homogenous and robust cardiomyocyte tissue cultures with good long-term viability. Ventricular heart cells prepared from fetal rats at embryonic day 13 were cultured in a scaffold-free two-step process. To optimize the cell culture model, several digestion protocols and culture conditions were tested. After digestion of fetal cardiac ventricles, the resultant cell suspension of isolated cardiocytes was shaken to initialize cell aggregate formation. In the second step, these three-dimensional cell aggregates were transferred onto a microporous membrane to allow further microstructure formation. Autonomously beating cultures possessed more than 25 cell layers and a homogenous distribution of cardiomyocytes without central necrosis after 8 weeks in vitro. The cardiomyocytes showed contractile elements, desmosomes, and gap junctions analyzed by immunohistochemistry and electron microscopy. The beat frequency could be modulated by adrenergic agonist and antagonist. Adenoviral green fluorescent protein transfer into cardiomyocytes was possible and highly effective. This three-dimensional tissue model proved to be useful for studying cell-cell interactions and cell differentiation processes in a three-dimensional cell arrangement.
An Improved Single-Stage Flyback PFC Converter for High-Luminance Lighting LED Lamps (2007)

This paper presents a universal power supply for high-luminance LED lamp test. The proposed power supply is designed and implemented by adopting an improved single-stage Flyback configuration. A constant current control method is used to regulate the lamp current and brightness under different pieces of LED series connection. A laboratory prototype has been built and tested. With the prototype, high efficiency, high power factor, and constant lamp current can be achieved for the performance test of various LED lamps.
Proactive Desk II: Development of a New Multi-object Haptic Display Using a Linear Induction Motor (2006)

The Proactive Desk is a force feedback system using a two-degree of freedom linear induction motor (LIM) designed for ordinary desktop operations. The system provides two-dimensional force feedback on a desktop surface, but only for a single object. This means that when several users operate this system together, only one user at a time can acquire force feedback from the system. In this paper, we propose a new-generation haptic display, Proactive Desk II, which uses an advanced LIM for multi-object driving, and develop a prototype system based on our approach. The system employs a cluster of coils for producing magnetic fluxes individually underneath the desktop, and those synthesize several traveling magnetic fields as a result. These magnetic fields simulate a local region of the field generated by our first-generation system, being able to drive multiple objects separately.
LE SEPARATEUR TRANSPOSABLE (1985)

L'ARTICLE PRESENTE LE SEPARATEUR TRANSPOSABLE QUI EST UN DISPOSITIF DE PROTECTION LIE A LA REALISATION DES CHANTIERS LOURDS SUR AUTOROUTE. LE DISPOSITIF COMPORTE 2 ELEMENTS : LE SEPARATEUR PROPREMENT DIT ET LA MACHINE DE TRANSFERT. L'ORIGINE ET LES CARACTERISTIQUES GENERALES SONT TOUT D'ABORDS PRESENTEES. LES MODIFICATIONS APPORTEES AUX PROTOTYPES POUR PALLIER CERTAINS INCONVENIENTS, AINSI QUE LES DEVELOPPEMENTS FUTURS FONT L'OBJET DE LA SUITE DE L'ARTICLE.