gebhart/patrepeval huggingface dataset:
Glycosidase activities of the 293 and NS0 cell lines, and of an antibody‐producing hybridoma cell line (1994)

We have previously demonstrated that Chinese hamster ovary (CHO) cell lysates harbor sialidase, beta-galactosidase, beta-hexosaminidase, and fucosidase activities that can accumulate extracellularly in CHO cell culture, thereby potentially leading to extracellular modification of glycoprotein oligosaccharides. The sialidase activity in CHO cell lysates was surprisingly active and stable at pH 7.5, with a half-life of 57 h at 37 degrees C.We have extended this work to determine whether 293, NS0, or hybridoma cell lysates contain similar glycosidase activities. The pH-activity profiles of beta-galactosidase and beta-hexosaminidase in lysates of these three cell lines resemble the pH-activity profiles for these enzymes in CHO cell lysate, whereas the pH-activity profiles of sialidase and fucosidase appear to be cell-type dependent. Sialidase activities were relatively stable at pH 4.5 in 293, NS0, and hybridoma cell lysates. However, the activities in 293 and NS0 cell lysates were unstable at pH 7.5, with no activity remaining after a 2-h incubation at 37 degrees C. The sialidase activity in hybridoma cell lysate was moderately stable at pH 7.5 with 30% of the activity remaining after a 2-h incubation at 37 degrees C. We conclude that the sialidase activites from 293, NS0, and hybridoma cells have characteristics similar to the vast majority of reported mammalian sialidase activities, and that these activities are markedly differant from the CHO cell sialidase activity.Finally, sialidase, beta-galactosidase, beta-hexosaminidase, and fucosidase activities were measured at pH 7 in cell-free bioreactor supernatants of the hybridoma cell line. As previously observed in CHO cell culture, all four glycosidase activities were present in the hybridoma supernatants. However, the sialidase activity in hybridoma supernatant was an order of magnitude lower than in CHO cell culture supernatant despite the fact that the hybridoma cell lysis rate was an order of magnitude higher. (c) 1994 John Wiley & Sons, Inc.
Evaluation of simplified dna extraction methods for<i>EMM</i>typing of group a streptococci (2006)

Simplified methods of DNA extraction for amplification and sequencing for emm typing of group A streptococci (GAS) can save valuable time and cost in resource crunch situations. To evaluate this, we compared two methods of DNA extraction directly from colonies with the standard CDC cell lysate method for emm typing of 50 GAS strains isolated from children with pharyngitis and impetigo. For this, GAS colonies were transferred into two sets of PCR tubes. One set was preheated at 94 degrees C for two minutes in the thermal cycler and cooled while the other set was frozen overnight at -20 degrees C and then thawed before adding the PCR mix. For the cell lysate method, cells were treated with mutanolysin and hyaluronidase before heating at 100 degrees C for 10 minutes and cooling immediately as recommended in the CDC method. All 50 strains could be typed by sequencing the hyper variable region of the emm gene after amplification. The quality of sequences and the emm types identified were also identical. Our study shows that the two simplified DNA extraction methods directly from colonies can conveniently be used for typing a large number of GAS strains easily in relatively short time.
Expression, Perception, and Induction of Musical Emotions: A Review and a Questionnaire Study of Everyday Listening (2004)

In this article, we provide an up-to-date overview of theory and research concerning expression, perception, and induction of emotion in music. We also provide a critique of this research, noting that previous studies have tended to neglect the social context of music listening. The most likely reason for this neglect, we argue, is that that most research on musical emotion has, implicitly or explicitly, taken the perspective of the musician in understanding responses to music. In contrast, we argue that a promising avenue toward a better understanding of emotional responses to music involves diary and questionnaire studies of how ordinary listeners actually use music in everyday life contexts. Accordingly, we present findings from an exploratory questionnaire study featuring 141 music listeners (between 17 and 74 years of age) that offers some novel insights. The results provide preliminary estimates of the occurrence of various emotions in listening to music, as well as clues to how music is used by listeners in a number of different emotional ways in various life contexts. These results confirm that emotion is strongly related to most people's primary motives for listening to music.
Genotypic Changes in Human Immunodeficiency Virus Type 1 Protease Associated with Reduced Susceptibility and Virologic Response to the Protease Inhibitor Tipranavir (2006)

Tipranavir is a novel, nonpeptidic protease inhibitor of human immunodeficiency virus type 1 (HIV-1) with activity against clinical HIV-1 isolates from treatment-experienced patients. HIV-1 genotypic and phenotypic data from phase II and III clinical trials of tipranavir with protease inhibitor-experienced patients were analyzed to determine the association of protease mutations with reduced susceptibility and virologic response to tipranavir. Specific protease mutations were identified based on stepwise multiple-regression analyses of phase II study data sets. Validation included analyses of phase III study data sets to determine if the same mutations would be selected and to assess how these mutations contribute to multiple-regression models of tipranavir-related phenotype and of virologic response. A tipranavir mutation score was developed from these analyses, which consisted of a unique string of 16 protease positions and 21 mutations (10V, 13V, 20M/R/V, 33F, 35G, 36I, 43T, 46L, 47V, 54A/M/V, 58E, 69K, 74P, 82L/T, 83D, and 84V). HIV-1 isolates displaying an increasing number of these tipranavir resistance-associated mutations had a reduced phenotypic susceptibility and virologic response to tipranavir. Regression models for predicting virologic response in phase III trials revealed that each point in the tipranavir score was associated with a 0.16-log10 copies/ml-lower virologic response to tipranavir at week 24 of treatment. A lower number of points in the tipranavir score and a greater number of active drugs in the background regimen were predictive of virologic success. These analyses demonstrate that the tipranavir mutation score is a potentially valuable tool for predicting the virologic response to tipranavir in protease inhibitor-experienced patients.
Chemical sensors technology development planning workshop (1993)

The workshop participants were asked to: (1) Assess the current capabilities of chemical sensor technologies for addressing US Department of Energy (DOE) Environmental Restoration and Waste Management (EM) needs; (2) Estimate potential near term (one to two years) and intermediate term (three to five years) capabilities for addressing those needs; and (3) Generate a ranked list of specific recommendations on what research and development (R&D) should be funded to provide the necessary capabilities. The needs were described in terms of two pervasive EM problems, the in situ determination of chlorinated volatile organic compounds (VOCs), and selected metals in various matrices at DOE sites. The R&D recommendations were to be ranked according to the estimated likelihood that the product technology will be ready for application within the time frame it is needed and the estimated return on investment. The principal conclusions and recommendations of the workshop are as follows: Chemical sensors capable of in situ determinations can significantly reduce analytical costs; Chemical sensors have been developed for certain VOCs in gases and water but none are currently capable of in situ determination of VOCs in soils; The DOE need for in situ determination of metals in soils cannot be addressed with existing chemical sensors and the prospects for their availability in three to five years are uncertain; Adaptation, if necessary, and field application of laboratory analytical instruments and those few chemical sensors that are already in field testing is the best approach for the near term; The chemical sensor technology development plan should include balanced support for near- and intermediate-term efforts.
Ultimate intrinsic signal‐to‐noise ratio in MRI (1998)

A method to calculate the ultimate intrinsic signal-to-noise ratio (SNR) in a magnetic resonance experiment for a point inside an arbitrarily shaped object is presented. The ultimate intrinsic SNR is determined by body noise. A solution is obtained by optimizing the electromagnetic field to minimize total power deposition while maintaining a constant right-hand circularly polarized component of the magnetic field at the point of interest. A numerical approximation for the optimal field is found by assuming a superposition of a large number of plane waves. This simulation allowed estimation of the ultimate intrinsic SNR attainable in a human torso model. The performance of six coil configurations was evaluated by comparing the SNR of images obtained by the coils with the ultimate values. In addition, the behavior of ultimate intrinsic SNR was investigated as a function of main field strength. It was found that the ultimate intrinsic SNR increases better than linearly with the main magnetic field up to 10 T for our model. It was observed that for field strengths of 4 T or higher, focusing is required to reach the ultimate intrinsic SNR.
High speed data transmissions from pole to buildings (1993)

Numerous techniques have been investigated to increase the capacity of mobile radio systems, the most effective being the deployment of microcells. A microcell is the coverage area provided when the base station antenna is below the height of the surrounding (sub)urban sky-line. Typically antenna heights between 3 and 12 metres are used. The shape of the microcell is determined by the topology of the buildings surrounding the antenna, with the dimensions of the microcell dependent on the transmitter power. The authors conducted measurements using base stations situated at a low elevation on lamp-posts or utility poles in the streets to provide radio communications access to a number of adjacent houses. A digital CPM modem operating at data rates of 2, 4 and 8 Mb/s at carrier frequencies of 1.85, 2.43 and 3.55 GHz was used. The authors show that high bit rate transmission into buildings from close access points appears feasible. >
Obstructive Sleep Apnea Disparity among Truck Drivers: Danger on American Roads (2018)

Obstructive sleep apnea (OSA), a common sleep disorder, can cause perilous levels of fatigue that impact commercial drivers who operate vehicles such as tow trucks, tractor trailers, and buses. In this study, we explored this population for its awareness of obstructive sleep apnea, the risk factors, and its impact on health for all. We conducted a qualitative study using 33 semi-structured in-depth interview questions with 12 male commercial truck drivers ages 28 to 44 years old, who were Florida residents. Participants had basic knowledge of OSA, minimal awareness of the underlying risk factors that impact their overall health, and of OSA treatment. There was awareness of the effect of OSA on sleep partners of study participants. All participants felt it was important for truck drivers to receive routine OSA screening, even among those who expressed concern about having results negatively impact their occupational status. Consistent policies of trucking companies, which ensure trucker involvement and maintenance of the necessary preventive behaviors, are necessary. Further, truck drivers and their families should receive direct and consistent support of health conditions that have a direct effect on the driver’s health and the industry’s future.
Selected applications of methotrexate alone and in combination in osteosarcoma. (1981)

Review of the reported use of high-dose methotrexate (HDMTX) with citrovorum factor rescue (CF) for treatment of primary and metastatic osteosarcoma indicates the rarity of responses in clinical situations other than treatment of the primary tumor or isolated pulmonary metastases with doses of 7.5 g/m2 or greater delivered on a weekly basis for 4 to 6 weeks. Adjuvant chemotherapy using HDMTX-CF at triweekly intervals has led to 2-year disease-free survival rates that are inferior to those obtained with combinations of agents including HDMTX-CF Adriamycin (Adria), with or without vincristine, cyclophosphamide, phenylalanine mustard, bleomycin, and dactinomycin. We concluded that although HDMTX-CF has a role in the treatment of primary and metastatic osteosarcoma, its use in combination with other active agents offers greater potential advantage to affected patients.
Model-based synthesis of fault trees from Matlab-Simulink models (2002)

We outline a new approach to safety analysis in which concepts of computer HAZOP are fused with the idea of software fault tree analysis to enable a continuous assessment of an evolving programmable design developed in Matlab-Simulink. We also discuss the architecture of a tool that we have developed to support the new method and enable its application in complex environments. We show that the method and the tool enable the integrated hardware and software analysis of a programmable system and that in the course of that analysis they automate and simplify the development of fault trees for the system. Finally, we propose a demonstration of the method and the tool and we outline the experimental platform and aims of that demonstration.
Cholinergic mechanisms in pain and analgesia. (1989)

There is now substantial evidence that acetylcholinesterase inhibitors and muscarinic receptor agonists increase the pain threshold after both systemic and spinal administration. In rats, physostigmine gave a significant dose-dependent increase in latency times in the tail immersion test following intrathecal administration. The effect was antagonized with atropine. Neostigmine gave more prolonged latencies as did the muscarinic receptor agonist carbachol. Spinal cholinergic pathways for antinociception interacted with the spinal opioid and adrenergic nerve tracts. No cross-tolerance to the selective alpha 2-adrenoreceptor agonist guanfacine or to morphine was seen in rats tolerant of spinal carbachol antinociception. The mechanism of spinal cholinergic antinociception is not known but a muscarinic interneuron may explain the interactions with other neurotransmitters. Clinically, the centrally active cholinesterase inhibitor physostigmine has been shown to give postoperative pain relief although of short duration. Severe neurogenic pain has been successfully treated with physostigmine or distigmine.
Saccharide imprinting of poly(aniline boronic acid) in the presence of fluoride (2003)

A new approach for the electrosynthesis of saccharide-imprinted poly(aniline boronic acid) is described. The method involves the formation of a saccharide–aminophenylboronic acid complex in the presence of fluoride to allow the electropolymerization of a self-doped, molecularly imprinted polyaniline. The formation of the anionic monomer complex enables electrochemical polymerization at near neutral pH (5–7) ensuring the incorporation of saccharide in the resulting, self-doped polymer. In this work, films were imprinted with D-fructose where saccharide–aminophenylboronic acid complexation occurred in the presence of one equivalent of fluoride. The selectivity toward D-fructose relative to D-glucose showed an increase of over 25% as a result of imprinting. In addition to the enhanced selectivity, to the best of our knowledge this is the first example of the electropolymerization of a self-doped polyaniline homopolymer under neutral pH conditions.
Short duration synchronization of human theta rhythm during recognition memory (1997)

Structures within the medial temporal lobe, particularly the hippocampus, have long been implicated in human episodic memory. The same structures are known to generate EEG in the theta frequency range in animals. The aim of this experiment was to investigate the time course of changes in the human theta rhythm during a word recognition memory task. In the period 125-250 ms after the visual presentation of a word, theta power increased by an average of 13% compared with the prestimulus baseline period and this increase was more than twice as great for repeated words (18%) as for new ones (8%). These results show that there are short duration changes in the human theta rhythm associated with recognition memory.
Laser speckle measurements of transient Bénard convection (1978)

Quantitative velocity measurements of unsteady convection in a Bénard cell suddenly cooled from above to a supercritical Rayleigh number are reported. The results are obtained by a novel application of laser speckle photography adapted for the study of fluid dynamics. This technique allows two-dimensional full-field velocity information to be obtained solely from a selected plane within the volume of moving liquid at any given instant in time. The convective rolls are found to be regular and oriented parallel to the short side of the cell. The number of rolls within the cell is approximately twice as great as the number reported for steady conditions. The maximum vertical velocity distribution is observed to be a sinusoidal function of the horizontal distance with a detectable third-harmonic component.
Quality factors for degraded proton spectra (1965)

Quality factors for degraded proton spectra from ratio of dose equivalent to absorbed dose - radiation dose measurements in skin
ChemInform Abstract: STEREOSPECIFIC SYNTHESIS OF THE ANTICANCER AGENT 5′‐DEOXY‐5‐FLUOROURIDINE (5‐DFUR) AND ITS 5′‐DEUTERATED DERIVATIVES (1982)

Abstract Die Titelverbindung (IIIb) wird in hoher Ausb. durch stereospezifische Kondensation der triacetylierten Desoxyribose (Ia) (Anomerengemisch) mit dem Fluoruracilderivat (II) [→(IIIa)] und nachfolgende Desacetylierung erhalten.
THE DIFFERENTIAL DETERMINATION OF CONJUGATED HYDROXYSKATOLES IN HUMAN URINE (1967)

A method is described for the separation and estimation of 4-, 5-, 6-, and 7-hydroxyskatole sulfate esters in urine. After extraction from urine, the 5-, 6-, and 7-hydroxyskatole sulfate esters were hydrolyzed by a sulfatase preparation to the corresponding hydroxyskatoles. The 4-isomer was resistant to this enzymic hydrolysis. The hydroxyskatoles were then extracted by ethyl acetate from the hydrolyzate followed by extraction of the 4-sulfate ester by n-butanol. Each fraction was chromatographed on silica gel G thin layers, sprayed with a modified Ehrlich's reagent (p-N,N-bis(2-chloroethyl)aminobenzaldehyde), and the appropriate zones eluted from the chromatogram. The concentrations of each isomer originally present in the urine were estimated from the absorbances of the four eluates at their absorption maxima (ca. 600 mμ). The levels of the four isomeric skatolyl sulfates found in urines from 10 normal controls are given.
Electrochemical Sensors and Biosensors Based on Nanomaterials and Nanostructures (2014)

We report that considerable attention has been devoted to the integration of recognition elements with electronic elements to develop electrochemical sensors and biosensors.Various electrochemical devices, such as amperometric sensors, electrochemical impedance sensors, and electrochemical luminescence sensors as well as photoelectrochemical sensors, provide wide applications in the detection of chemical and biological targets in terms of electrochemical change of electrode interfaces. Here, this review focuses on recent advances in electrochemical sensors and biosensors based on nanomaterials and nanostructures during 2013 to 2014. The aim of this effort is to provide the reader with a clear and concise view of new advances in areas ranging from electrode engineering, strategies for electrochemical signal amplification, and novel electroanalytical techniques used in the miniaturization and integration of the sensors. Moreover, the authors have attempted to highlight areas of the latest and significant development of enhanced electrochemical nanosensors and nanobiosensors that inspire broader interests across various disciplines. Electrochemical sensors for small molecules, enzyme-based biosensors, genosensors, immunosensors, and cytosensors are reviewed herein (Figure 1). Such novel advances are important for the development of electrochemical sensors that open up new avenues and methods for future research. In conclusion, we recommend readers interested in the general principles of electrochemical sensors and electrochemical methods to refer to other excellent literature for a broad scope in this area.(3, 4) However, due to the explosion of publications in this active field, we do not claim that this Review includes all of the published works in the past two years and we apologize to the authors of excellent work, which is unintentionally left out.
Free cytoplasmic Ca <sup>++</sup> at rest and after cholinergic stimulus is increased in cultured muscle cells from Duchenne muscular dystrophy patients (1988)

We used a fluorescent dye, quin 2, to measure intracellular free calcium ([Ca++]i) in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++]i level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++]i transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggests that there is no generalized membrane defect.
Shufflons: Multiple Inversion Systems and Integrons (1999)

Conservative site-specific recombination functions to create biological diversity in prokaryotes. Simple site-specific recombination systems consist of two recombination sites and a recombinase gene. The plasmid R64 shufflon contains seven recombination sites, which flank and separate four DNA segments. Site-specific recombinations mediated by the product of the rci gene between any two inverted recombination sites result in the inversion of four DNA segments independently or in groups. The shufflon functions as a biological switch to select one of seven C-terminal segments of the PilV proteins, which is a minor component of R64 thin pilus. The shufflon determines the recipient specificity in liquid matings of plasmid R64. Other multiple inversion systems as well as integrons, which are multiple insertion systems, are also described in this review.