gebhart/patrepeval huggingface dataset:
An atrial peptide is a potent renal vasodilator substance. (1984)

Renal intra-arterial administration of rat atrial extracts elicits a concentration dependent renal vasodilation (independent of prostaglandin or dopamine release) in anesthetized rats. The atrial extracts do not alter skeletal musculature (hindlimb) vascular resistance or systemic arterial blood pressure. The high molecular weight peptide fraction of atrial extracts obtained by gel filtration, reduces renal resistance intra-arterially only after proteolytic activation (in vitro) or following intravenous (i.e., systemic in vivo activation) administration. The low molecular weight peptide fraction of the atrial extract which is active intra-arterially as a renal vasodilator has been further purified to yield two major peptides. The 21 amino acid peptide, designated atriopeptin I, was previously demonstrated to be natriuretic and to relax intestinal but not vascular smooth muscle strips. This peptide exerted little or no intra-arterial effect on renal resistance. The 23 amino acid peptide (the phenylalanine-arginine C terminal extension of atriopeptin I), designated atriopeptin II, was natriuretic and spasmolytic (in vitro) on both intestinal and vascular strips and was a potent renal vasodilator in vivo. Thus, the renal vasodilator activity present in cardiac atrial extracts appears to derive from a proteolytic process which selectively generates the 23 amino acid peptide, atriopeptin II. Further cleavage with the loss of phenylalanine-arginine C-terminal, as occurs with atriopeptin I, markedly suppresses the renal vasodilation.
Are Nutritional Supplements Ready for Prime Time? (2008)

Article AbstractBecause this piece has no abstract, we have provided for your benefit the first 3 sentences of the full text.Over the past 20 years, there has been a surge of clinical trials examining the safety and efficacy of natural compounds for psychiatric indications. These studies have produced encouraging evidence for several putative antidepressants such as S-adenosyl-L-methionine (SAMe), St. John's wort (SJW), the omega-3 fatty acids, and folic acid.There are about 45 published randomized controlled trials(RCTs) of SAMe monotherapy against placebo or tricyclic antidepressants(TCAs).
Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96‐well microplates (2005)

Quantification of SC by means of a 96-well microplate-based colorimetric method is feasible and shortens the time of analysis. However, when using D-Squame tape disks, SC removal on a limited area of the tape is not predictive for SC removal on the entire tape as removal is inhomogenous. Therefore, SC protein extraction should be performed on a large enough area, eventually on the entire tape when quantifying SC mass removed by tape stripping.
Analysis of Micrometer Sample Flows in a Non Coaxial Sheath Flow Device (2007)

In this paper we present a novel non coaxial sheath flow device with channel dimensions of 50 μm in square at the focusing region. Analyses of the adaptable sample flow are carried out where the focusing limitation and its profile variations are shown using confocal microscope measurements. A comparison of the sample flow height inside the channel and its relative fluorescence intensity is highlighted. Additionally, the influence of the hydrodynamic focusing on the sample profile and its uniformity over a certain distance is shown.
Isolation and Characterization of a Highly Specific Serine Endopeptidase from an Oral Strain of Staphylococcus epidermidis (2001)

Infection by Staphylococcus epidermidis, an opportunistic pathogen, has become a major problem due to the increased use of implanted medical devices and the growing number of patients who are therapeutically or infectiously immunosuppressed. These infections appear to proceed via modulation of the coagulation and complement systems. In this communication we describe the purification and characterization of a novel extracellular proteinase from an oral strain of S. epidermidis that can degrade fibrinogen, complement protein C5, and several other proteins. This proteinase has a strong preference for cleavage after glutamic acid residues, but not after aspartic acid. The S. epidermidis enzyme may be a multifunctional protein which not only provides this organism with both the ability to evade the complement defense system and to dysregulate the coagulation cascade, but also supplies nutrients for its growth through the degradation of Glu-rich proteins.
Mobile Robot Indoor Positioning Based on a Combination of Visual and Inertial Sensors (2019)

Multi-sensor integrated navigation technology has been applied to the indoor navigation and positioning of robots. For the problems of a low navigation accuracy and error accumulation, for mobile robots with a single sensor, an indoor mobile robot positioning method based on a visual and inertial sensor combination is presented in this paper. First, the visual sensor (Kinect) is used to obtain the color image and the depth image, and feature matching is performed by the improved scale-invariant feature transform (SIFT) algorithm. Then, the absolute orientation algorithm is used to calculate the rotation matrix and translation vector of a robot in two consecutive frames of images. An inertial measurement unit (IMU) has the advantages of high frequency updating and rapid, accurate positioning, and can compensate for the Kinect speed and lack of precision. Three-dimensional data, such as acceleration, angular velocity, magnetic field strength, and temperature data, can be obtained in real-time with an IMU. The data obtained by the visual sensor is loosely combined with that obtained by the IMU, that is, the differences in the positions and attitudes of the two sensor outputs are optimally combined by the adaptive fade-out extended Kalman filter to estimate the errors. Finally, several experiments show that this method can significantly improve the accuracy of the indoor positioning of the mobile robots based on the visual and inertial sensors.
Unusual macromolecular architectures: the convergent growth approach to dendritic polyesters and novel block copolymers (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUnusual macromolecular architectures: the convergent growth approach to dendritic polyesters and novel block copolymersCraig J. Hawker and Jean M. J. FrechetCite this: J. Am. Chem. Soc. 1992, 114, 22, 8405–8413Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/ja00048a009https://doi.org/10.1021/ja00048a009research-articleACS PublicationsRequest reuse permissionsArticle Views942Altmetric-Citations160LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Expression of Telomerase Extends the Lifespan and Enhances Osteogenic Differentiation of Adipose Tissue–Derived Stromal Cells (2004)

Expression of TERT, the catalytic protein subunit of the telomerase complex, can be used to generate cell lines that expand indefinitely and retain multilineage potential. We have created immortal adipose stromal cell lines (ATSCs) by stably transducing nonhuman primate-derived ATSCs with a retroviral vector expressing TERT. Transduced cells (ATSC-TERT) had an increased level of telomerase activity and increased mean telomere length in the absence of malignant cellular transformation. Long-term culture of the ATSC-TERT cells demonstrated that the cells retain the ability to undergo differentiation along multiple lineages such as adipogenic, chondrogenic, and neurogenic. Untransduced cells demonstrated markedly reduced multilineage and self-renewal potentials after 12 passages in vitro. To determine the functional role of telomerase during osteogenesis, we examined osteogenic differentiation potential of ATSC-TERT cells in vitro. Compared with naive ATSCs, which typically begin to accumulate calcium after 3-4 weeks of induction by osteogenic differentiation medium, ATSC-TERT cells were found to accumulate significant amounts of calcium after only 1 week of culture in osteogenic induction medium. The cells have increased production of osteoblastic markers, such as AP2, osteoblast-specific factor 2, chondroitin sulfate proteoglycan 4, and the tumor necrosis factor receptor superfamily, compared with control ATSCs, indicating that telomerase expression may aid in maintaining the osteogenic stem cell pool during in vitro expansion. These results show that ectopic expression of the telomerase gene in nonhuman primate ATSCs prevents senescence-associated impairment of osteoblast functions and that telomerase therapy may be a useful strategy for bone regeneration and repair.
Subpopulations of normal peripheral blood and bone marrow cells express a functional multidrug resistant phenotype [see comments] (1992)

The multidrug-resistance gene, MDR1 is expressed in many normal tissues, but little is known about its expression in normal hematopoietic cells. Using the monoclonal antibody C219 and flow cytometric analysis, P-glycoprotein (P-gp) was found to be expressed in all peripheral blood (PB) subpopulations (CD4, CD8, CD14, CD19, CD56) except granulocytes. To specifically determine MDR1 gene expression, these PB subpopulations were isolated by fluorescence-activated cell sorting (FACS) and analyzed for MDR1 mRNA by polymerase chain reaction (PCR). All subsets were positive by PCR, but only minimal MDR1 mRNA was detected in monocytes and granulocytes. Significant efflux of Rhodamine- 123 (Rh-123), a measure of P-gp function, was detected in CD4+, CD8+, CD14+, CD19+, and CD56+ cells but not in granulocytes. Next, PCR- analysis was performed on FACS-sorted bone marrow (BM) cells to assess MDR1 expression in different maturational stages. Precursors (CD34+), early and late myeloid cells (CD33+/CD34+, CD33+/CD34-) as well as lymphocytes of the B-cell lineage (CD19+/CD10+, CD19+/CD10-) expressed the MDR1 gene. BM monocytic cells (CD33++/CD34-) were negative, and a very weak signal was detected in erythroid cells (glycophorin A+). Significant Rh-123 efflux was found in CD34+, CD10+, CD33+, and CD33++ BM cells, but not in glycophorin A+ cells. We conclude that PB and BM lymphocytes, PB monocytes, BM progenitors, and immature myeloid cells, but not late BM monocytes, erythroid cells, and PB granulocytes, express MDR1 mRNA and a functional P-gp. These results have to be taken into account when MDR1 expression is determined in tumor samples containing normal blood cells.
Connection between inflammation and carcinogenesis in gastrointestinal tract: Focus on TGF-β signaling (2010)

Inflammation is a primary defense process against various extracellular stimuli, such as viruses, pathogens, foods, and environmental pollutants. When cells respond to stimuli for short periods of time, it results in acute or physiological inflammation. However, if the stimulation is sustained for longer time or a pathological state occurs, it is known as chronic or pathological inflammation. Several studies have shown that tumorigenesis in the gastrointestinal (GI) tract is closely associated with chronic inflammation, for which abnormal cellular alterations that accompany chronic inflammation such as oxidative stresses, gene mutations, epigenetic changes, and inflammatory cytokines, are shared with carcinogenic processes, which forms a critical cross-link between chronic inflammation and carcinogenesis. Transforming growth factor (TGF)-beta is a multi-potent cytokine that plays an important role in regulation of cell growth, apoptosis and differentiation. Most importantly, TGF-beta is a strong anti-inflammatory cytokine that regulates the development of effector cells. TGF-beta has a suppressive effect on carcinogenesis under normal conditions by inhibiting abnormal cell growth, but on the other hand, many GI cancers originate from uncontrolled cell growth and differentiation by genetic loss of TGF-beta signaling molecules or perturbation of TGF-beta adaptors. Once a tumor has developed, TGF-beta exerts a promoting effect on the tumor itself and stromal cells to enhance cell growth, alter the responsiveness of tumor cells to stimulate invasion and metastasis, and inhibited immune surveillance. Therefore, novel development of therapeutic agents to inhibit TGF-beta-induced progression of tumor and to retain its growth inhibitory activities, in addition to anti-inflammatory actions, could be useful in oncology. In this review, we discuss the role of TGF-beta in inflammation and carcinogenesis of the GI tract related to abnormal TGF-beta signaling.
Quasi-phase-matched second-harmonic generation in AlGaAs waveguides with periodic domain inversion achieved by wafer-bonding (1995)

Quasi-phase-matched second-harmonic generation is observed in an AlGaAs waveguide. The AlGaAs waveguide is epitaxially grown on a template substrate where a periodic crystal domain inversion is achieved using wafer bonding and organometallic chemical vapor deposition. A scanning electron micrograph of the waveguide cross section reveals a distinct propagation of the crystal domain boundaries in the epitaxial growth direction. Second-harmonic generation measurements on a fabricated rib-loaded waveguide show a clear quadratic dependence of the second-harmonic power to the input fundamental power. The peak conversion efficiency is 4.9%/W whereas the theoretical value is 124%/W for an ideal waveguide with no loss and with equal domain dimensions. A significant increase in the conversion efficiency is expected with reduced scattering losses realized by improved epitaxial growth and fabrication processes.
Preliminary definition of a “critical region” of chromosome 13 in q32: Report of 14 cases with 13q deletions and review of the literature (1993)

We report on 14 patients with partial deletions of chromosome 13q. These patients exhibit a wide spectrum of phenotypes. Deletions limited to proximal bands q13-q31 are associated with growth retardation but not with major malformations. We review the literature since 1975 and summarize 13q deletion cases which have a phenotype involving one or more major malformations and mental retardation. Analysis of the breakpoints of these cases, as well as those reported by us, supports the hypothesis that only deletions involving at least part of band q32 are associated with major malformations and digital abnormalities. Patients with more distal deletions have severe mental retardation but do not have major malformations or growth retardation. A group of patients in whom the breakpoint is stated to be within q32 has had an intermediate phenotype. This suggests that it may be possible to define subregions within q32 whose deletion is associated with particular developmental defects.
Social Media Driven Public Health Informatics: Applications in Regulatory Science (2019)

Health information technology (health IT) is an emerging interdisciplinary research field that brings innovative and unique opportunities as well as challenges for information systems (IS) researchers. Public Health Informatics (PHI) is an important subdomain of health IT but yet extensively studied by IS researchers. The limited IS literature on PHI surveillance, especially on regulatory science opens new gates for IS researchers to test existing and develop new theories, to design and configure innovative methods and models, and to provide practical health managerial insights. The emerging information technology innovations provide novel insights and opportunities to improve public health surveillance. Specifically, social media analytics and intelligence, broadly accepted and applied in the current IS domains, has motivated a new branch of research in regulatory science that may bear prolific fruits both in theoretical and pragmatic perspectives. This dissertation seeks to address the problem and fills the research gaps by proposing a systematic research framework for regulatory science surveillance using social media-driven approaches. Five essays in the design science paradigm are developed. Essay I aims to provide a basic understanding of social media user-generated content on regulated products by text mining and social network analysis techniques. User-generated content can further motivate research on the user level. Essay II takes this perspective and models user features based on text. Essay III focuses on another important feature of social media – network structures, and develops a computational algorithm for large-scale social network analysis on modeling social influence. After proposing innovative IS solutions to real regulatory public health problems, essay IV endeavors to validate the use of social media datasets by combining survey and social media data for data triangulation. Finally, essay V, motivated by the keyword-based social media data collection processes, strives to automatically and accurately detect sensitive regulated product street names, utilizing rich text information in the ever-changing linguistic environment on social media. Beyond the practical insights of health decision support and solutions provided in each essay, this dissertation offers a systematic social media-driven regulatory science informatics research paradigm to guide future PHI and other analytical IS research.
On iris detection for mobile device applications (2014)

A novel transform called Gradient Direction Transform for fast detection of naturally curved items in digital images is described in this article. This general purpose image transform is defined to suit platforms with limited memory and processing footprints by utilizing only additions and simple shift and bitwise operations. We present this unique algorithmic approach in application to real world problems of iris detection. The new approach is tested on a large data set and the experiments show promising and superior performance compared to existing techniques.
Word programmer for NMR (1986)

A NMR programmer is described which is built around a logic idea and a set of specifications, rather than availability of special or new integrated circuits. We begin by discussing what a quintessential Touring machine can do to solve the problem of outputting words (or pulses) with the resolution of the system clock over arbitrarily long time intervals. We then illustrate a simple realization of this machine, with 4096 program lines, 25-ns resolution, two 16-bit output lines, the possibility of nesting loops down to the 256th level, and a component cost in the 102 $ range. A software ‘‘human’’ interface has been developed in an IBM-PC compatible (MS-DOS) environment, which simplifies the task of debugging the entire NMR spectrometer, and that of designing new experiments. While certainly overspecified for all traditional solid-state NMR experiments, our programmer may meet the requirements of recently suggested selective excitation and imaging experiments in liquids.
Evaluation of Memory Dysfunction Following Experimental Brain Injury Using the Morris Water Maze (1991)

Memory dysfunction, a common clinical feature of traumatic brain injury (TBI), is thought to be related to secondary damage of key anatomic structures in the brain, including the hippocampus. In the present study, we have characterized and evaluated a novel experimental paradigm using the Morris water maze (MWM) technique, to measure post-TBI memory retention after lateral (parasagittal) fluid percussion (FP) brain injury in rats. Male Sprague-Dawley rats (n = 37) received a total of 20 training trials over 2 days in the MWM. Two and a half hours after the last training trial, the animals received FP brain injury of moderate severity (2.3 atmospheres, n = 12), high severity (2.6 atm, n = 13), or no injury (n = 12). Forty-two hours after FP brain injury, we observed a highly sufficient memory dysfunction in animals from both injury groups compared to the uninjured group (p less than 0.001). The degree of memory dysfunction was found to be directly related to the severity of injury, with the high severity group scoring significantly worse than the moderately injured group (p = 0.15). In addition, hippocampal cell loss was observed after brain injury, but only unilaterally. These data suggest that lateral FP brain injury causes memory dysfunction possibly related to concurrent hippocampal cell loss and that posttraumatic memory deficits may be sensitively quantitated using the memory testing paradigm described.
Effect of miR-19a and miR-21 on the JAK/STAT signaling pathway in the peripheral blood mononuclear cells of patients with systemic juvenile idiopathic arthritis (2016)

Overexpression of the components of the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway are key factors of the pathogenic mechanisms underlying systematic juvenile idiopathic arthritis (SJIA). The present study aimed to investigate the association between microRNA (miR)-19a, miR-21 and the JAK/STAT signaling pathway. A total of 20 patients with SJIA were included in the study, and peripheral blood mononuclear cells (PBMCs) from 20 normal controls were also collected. RNAiso was used to extract total RNA, and the RNA was then reverse transcribed into cDNA. Primers were designed to detect the mRNA of miR-19a and miR-21, and U6 was set as the internal parameter. In addition, the mRNA of STAT3, suppressor of cytokine signaling 3 (SOCS3), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) was detected, and β-actin was set as the internal parameter. Reverse transcription-quantitative polymerase chain reaction was performed to detect the expression levels of these proteins in patients with SJIA and control subjects, and non-parametric tests were used to analyze the statistical differences in 2<sup>-ΔΔCq</sup> between the two groups. The expression levels of miR-19a and miR-21 were significantly lower in the SJIA group compared with the control group (P<0.05). SOCS3, TNF-α and STAT3 were shown to be the target genes of miR-19a and miR-21, as determined by Targetscan. The expression levels of STAT3, SOCS3, TNF-α and IL-6 mRNA were significantly higher compared with those of the control group (P<0.05). In the PBMCs of sthe patients with SJIA, miR-19a and miR-21 expression levels were lower compared with those of the control group, and the JAK/STAT signaling pathway was activated, which indicated that miR-19a and miR-21 may participate in the activation of the JAK/STAT signaling pathway.