gebhart/patrepeval huggingface dataset:
High Affinity Binding of β-Adrenergic Receptor Kinase to Microsomal Membranes (1996)

The beta-adrenergic receptor kinase (beta ARK) modulates beta-adrenergic and other G protein-coupled receptors by rapidly phosphorylating agonist-occupied receptors at the plasma membrane. We have recently shown that beta ARK also associates with intracellular microsomal membranes both "in vitro" and "in situ" (García-Higuera, I., Penela, P., Murga, C., Egea, G., Bonay, P., Benovic, J. L., and Mayor, F., Jr. (1994) J. Biol. Chem. 269, 1348-1355), thus suggesting a complex modulation of the subcellular distribution of beta ARK. In this report, we used recombinant [35S]methionine-labeled beta ARK to show that this kinase interacts rapidly with a high affinity binding site (Kd of 20 +/- 1 nM) present in salt-stripped rat liver microsomal membranes. Although beta ARK binding is not modulated by membrane preincubation with G protein activators, the activity of bound beta ARK toward rhodopsin or a synthetic peptide substrate was markedly enhanced upon stimulation of the endogenous heterotrimeric G proteins present in the microsomal membranes by AIF4- or mastoparan/guanosine 5'-(3-O-thio)triphosphate, thus strongly suggesting a functional link between these proteins and membrane-associated beta ARK. Interestingly, beta ARK association with microsomal membranes is not significantly affected by a fusion protein derived from the carboxyl terminus of beta ARK1 (the proposed location of the beta gamma subunit binding site), whereas it is markedly inhibited by fusion proteins corresponding to the amino-terminal region of the kinase. The main determinants of binding appear to be localized to an approximately 60-amino acid residue stretch (residues 88 to 145). Our results further indicate a functional relationship between beta ARK and heterotrimeric G proteins in different intracellular organelles, and suggest that additional proteins may be involved in modulating the cellular localization of the kinase through a new targeting domain of beta ARK.
Robust adaptive full-order observer design based on γ-positive real problem for speed sensorless vector controlled induction motors (2004)

This paper proposes a design of a robust adaptive full-order observer based on /spl gamma/-positive real problem for sensorless vector controlled induction motors. The adaptive full-order observer is known to become unstable in a major part of regenerating-mode and low-speed region, and this prevents the sensorless vector controller from operating an induction motor successfully. In this paper, designs of a observer gain and adaptive schemes based on /spl gamma/-positive real problem are proposed. Firstly, the error system of the adaptive full-order observer is reconsidered, requirements of adaptive full-order observer with stator resistance identifier are described. Next, in order to satisfy all requirement of the robust adaptive observer, the designs of the observer gain and adaptive schemes for speed and stator resistance are shown. Finally, several experimental results show the feasibility and the effectiveness of the proposed design strategy.
Transport of Electrons in a Strong Built-in Electric Field (1968)

The problem is discussed of the transport of electrons in the presence of a strong field due to fixed space charges, as in the depletion region of a p-n junction. It is found that the effective mobility, for small departures from equilibrium, is equal to the chordal hot-electron mobility which would result if the strong field were applied by external means.
Computer generated images from digital grid databases (2003)

The author describes the application of computer-generated images (CGI) in training and terrain analysis devices. Many of these images are generated by converting a digital grid map (x, y, z) grid database to a perspective view which is then displayed on a TV monitor. The author presents a unique mathematical approach for this conversion and a basic functional design for real-time (TV-rate) display of these images. A seascape image generated is also described where the database is a mathematical model. The proposed techniques have been applied to generate very realistic terrain and seascape images. Pseudonoise patterns have been used to enhance realism. It is concluded that this has been very effective in the foreground where one x/y grid element covers many pixels. The approach is limited to about a 20 degrees pitch down of the boresight. The computations required are primarily a series of accumulations.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Impact of Cancer on Children's Physical, Emotional, and Psychosocial Well-being (2009)

The diagnosis and treatment of cancer are a stressful and threatening experience, which can be emotionally devastating to children. Despite the improved prognosis, the course of cancer treatment has tremendous impact on children. This article aims to examine the impact of cancer on physical, emotional, and psychosocial well-being of Hong Kong Chinese children, an area of research that has been underrepresented in the literature. Ninety-eight Hong Kong Chinese children aged 7 to 15 years, admitted for treatment of cancer in 2 pediatric oncology units of 2 different hospitals, were invited to participate in the study. Findings from this study indicated that the children scored considerably high state anxiety on admission, and more than half of the participants presented some depressive symptoms during their stay in the hospital. Moreover, semistructured interviews indicated that nearly all children expressed different degrees of sadness and worry. The findings suggested that there is a room for improvement in existing nursing intervention regarding preparing children for hospitalization and treatment of cancer. There is an imperative need for nurses to evaluate appropriate nursing interventions that can help children resume their normal growth and development, in particular, to help them ease the physical, emotional, and psychological burden of life-threatening disease.
Rehabilitating Blind and Visually Impaired People: A psychological approach (1993)

Blindness: implications of the term. Models of rehabilitation.Psychological adjustment to sight loss. Perception as information-processing. The brain. Special client groups and their problems. Congenital blindness. Anxiety, stress and the learning process. Low Vision. The family. Taking care of yourself.
Variable Phase Bright-Field Contrast—An Alternative Illumination Technique for Improved Imaging in Transparent Specimens (2012)

In variable phase bright-field contrast, a bright-field image based on axial or concentric-peripheral light is optically superimposed with a phase-contrast image, so that typical details that are imminent in one or the other technique contribute to the resulting composite image. In particular, complex structured specimens consisting of high-density light absorbing details and additional low-density phase shifting components can be observed with improved clarity. As both partial images interfere with each other, fine details within thin specimens can be highlighted further by additional contrast effects based on interference. Haloing and shade-off are significantly reduced when compared with phase contrast carried out stand-alone. Our method is characterized by several technical means that are relevant for the high image quality that can be achieved: both illuminating light components associated with bright field and phase contrast are filtered at different colors and separated from each other so that they meet the specimen at different angles of incidence. The intensities of the phase-contrast- and bright-field-producing light can be selectively regulated so that the final image can be dominated by phase contrast or bright field, or be equalized. The condenser aperture diaphragm can be used for modulations of the image's appearance.
Transcriptional regulation of the HO-1 gene in cultured macrophages exposed to model airborne particulate matter (2003)

Respirable particulate matter generated during incomplete combustion of fossil fuels may principally target the cells found in the distal region of the lung. This study characterizes some of the effects that a model particulate matter has on the induction of heme oxygenase (HO)-1 in macrophages. HO-1 is a highly inducible stress response gene that has been demonstrated to modulate chemical, physical, and environmental stimuli. Cultured macrophages (RAW 264.7 cells) exposed continuously to a well-defined model of particulate matter (benzo[a]pyrene adsorbed onto carbon black) induced HO-1 gene expression in a time-dependent manner. Likewise, the addition of benzo[a]pyrene-1,6-quinone, a redox cycling metabolite of benzo[a]pyrene, to RAW cells also induced HO-1. This particle-induced gene expression of HO-1 was found to correlate with a corresponding increase in protein levels. Gene regulation studies were performed to delineate the transcriptional regulation of HO-1 after exposure to model particulate matter. Deletional analysis of the HO-1 gene and mutational analysis of activator protein (AP)-1 regulatory element on both distal enhancers demonstrated the importance of this transcriptional factor in mediating HO-1 gene transcription in response to model particulate matter. These results were supported by gel shift analysis demonstrating increased AP-1 binding activity after exposure to particulate matter. In summary, this study demonstrates that model particulate matter enhanced the expression of HO-1. This inductive process may be mediated by AP-1 activation of the regulatory elements on both the 5'-distal enhancers.
Predictability of Adequacy of Spinal Root Decompression Using Evoked Potentials (1991)

Dermatomal evoked potentials are being used to obtain accurate information concerning the viability of specific nerve root pathways and the quality and extent of nerve root decompression. Twelve patients with quantifiable weakness were selected and tested before, during, and after surgery. Nine patients showed dramatic intraoperative electrical improvement, including decreased latencies and/or increased amplitudes; all had postoperative relief of pain and motor return. Three patients showed variable intraoperative electrical responses with complete pain relief but no motor improvement. No patients had false-negative findings. Intraoperative monitoring of dermatomal evoked potentials appears to be of value in predicting the quality of spinal decompression. Similar monitoring of somatosensory evoked potentials alone cannot provide the specificity for individual root levels that is available by monitoring individual dermatomes.
Metabolism and cellular functions of IP6: a review. (2000)

Inositol hexaphosphate (IP6) has a demonstrably effective anti-cancer action against a variety of experimental tumors. However, the mechanisms of its actions are yet to be completely discerned. Studies in my laboratory have shown that IP6 is rather rapidly absorbed by rats in vivo. Ion exchange chromatography demonstrates the presence of inositol and IP1-6 in gastric epithelial cells as early as within 1 h of intragastric 3H-IP6 administration. The metabolized IP6, in the form of inositol and IP1 is transported via plasma and reaches distant organs as well as tumors. In rats, the urinary metabolites of IP6 are inositol and IP1. However, in humans 1-3% of total administered IP6 is excreted in the urine as IP6; the level shows a normal oscillation between 0.5-6 mg/L [F. Grases et al]. Investigations of the uptake and metabolism by a variety of cancer cell lines in vitro also demonstrate an instantaneous absorption of IP6. The rate and pattern at which IP6 is metabolized by cancer cells varies depending on the cell type. Intracellular inositols accumulated mostly (80-97%) in the cytosol as inositol and IP1-6. IP6 treatment of all the cell lines tested so far demonstrates that it is cytostatic and not cytotoxic. Along with inhibition of cell proliferation, there is enhanced differentiation of malignant cells to a more mature phenotype, often resulting in reversion to normal. Studies of the expression of tumor suppressor gene demonstrate up-regulation of wild type p53 and down-regulation of the mutant form. Since p53-mediated cell cycle arrest may be the direct result of induction of WAF-1 gene (p21WAF-1/CIP1), our studies demonstrate that IP6 up-regulates the expression of p21WAF-1/CIP1 in a dose-dependent manner. These data strongly point towards the involvement of signal transduction pathways, cell cycle regulatory genes, differentiation genes, oncogenes and perhaps, tumor suppressor genes in bringing about the observed anti-neoplastic action of IP6.
Growth-Hormone-Binding Protein in Patients with Acromegaly (1992)

The present study was undertaken to investigate the possible regulatory effect of chronic exposure to human growth hormone (hGH), in patients with acromegaly, on growth-hormone-binding protein (GH-BP). Nineteen patients with active acromegaly, before, during or after treatment, comprised the subjects of this study. Serum GH was measured by radioimmunoassay and GH-BP by a binding assay with dextran-coated charcoal separation. The specific binding of [125I]hGH (1 ng) obtained with 50 microliters serum was expressed as a percentage of total cpm. To evaluate the impact of the lower GH-BP on GH activity, we studied the effect of acromegalic serum on hGH displacement of [125I]hGH binding to GH receptors in rabbit liver membranes. Compared to normal controls (11.43 +/- 0.37%), the acromegalic patients had low serum levels of GH-BP (5.45 +/- 0.40%; p < 0.001), which correlated negatively with serum GH levels (p < 0.01). In 7 patients, GH-BP normalized within 2-3 months of successful therapy. The lower GH-BP was due to a reduction in binding capacity, whereas binding affinity remained unchanged. Acromegalic serum, with its low GH-BP, resulted in a shift to the left of the GH displacement curve when compared with normal human sera: IC50 values were 7.47 +/- 0.29 and 11.19 +/- 0.84 ng (p < 0.02) for acromegalic and normal human sera, respectively. We conclude that acromegaly is characterized by low levels of GH-BP due to a decrease in serum-binding capacity. The decrease in GH-BP may render the acromegalic serum GH relatively more active in the GH receptor assay.
[Apoptosis of K562 cells induced by nociceptin/orphanin FQ]. (2006)

The study was to investigate the proliferation and apoptosis effect of nociceptin/orphanin FQ (OFQ) on 562 cells in vitro. Inhibition of K562 cells proliferation was measured by MTT assay. Morphological assessment of apoptosis was performed with Wright staining and transmission electron microscope. The apoptosis peak was measured by flow cytometry. DNA fragmentation was visualized by agarose gel electrophoresis. The results showed that OFQ time-dependently and no-dose-dependently inhibited the proliferation of K562 cells at concentrations of 10(-6) - 10(-13) mol/L. Discrete maximum of cytolytic activity was detected at concentrations of 10(-6) - 10(-7), 10(-9), 10(-12) mol/L. Compared with the control group K562 cells, the cells treated with OFQ at concentration of 10(-9) mol/L for 72 hours showed typical characteristics of apoptosis under transmission electron microscope. Apoptosis peak was found by FCM at concentration of 0, 10(-7), 10(-8), 10(-9) mol/L of OFQ for 72 hours, apoptosis rates were 0%, 22.8%, 23.8% and 26.5% respectively. DNA agarose gel electrophoresis revealed nuclear fragmentation (DNA ladder). It is concluded that OFQ can inhibit the proliferation of K562 cells and induce the apoptosis in K562 cells.
Investigation of the Scaling Law on Cellulose Solution Prepared at Low Temperature (2008)

Cellulose was dissolved rapidly in a 9.5 wt % NaOH/4.5 wt % thiourea aqueous solution pre-cooled to -5 degrees C to prepare its concentrated solution, in which inclusion complexes (ICs) associated with cellulose, NaOH, thiourea, and water clusters were created. Physical gels could form in the cellulose solution at either high temperature or after long storage time, because of aggregation between the ICs. To clarify whether the Winter and Chambon theory could describe the gelation process of this complex system, we have investigated carefully the viscoelastic behavior of the cellulose solution with the advanced rheological expanded system (ARES). In the temperature range from 10 to 25 degrees C, we have successfully measured the loss tangent (tan delta) at the gel point according to the Winter and Chambon theory, showing the independence of tan delta on the frequency for the cellulose solution. The exponents of the scaling laws eta 0 proportional, variant epsilon-gamma and Ge proportional, variant epsilon z for the cellulose solution at 10 degrees C before and beyond the gel point were confirmed to be in agreement with the predicted values based on the percolation theory. The high sensitivity of the cellulose solution on temperature poses a limit for the application of the scaling law for the wide temperature range. The gel formed from the cellulose solution at 30 degrees C at long storage time could undergo a transition to a transparent liquid state after stirring at -5 degrees C. At the same time, the loss modulus (G' ') exceeds the storage modulus (G'), indicating a partially reversible sol-gel transition, as a result of the reconstruction of the hydrogen-bond networks between the solvent and cellulose.
Numerical investigation of implementation of air-earth boundary by acoustic-elastic boundary approach (2007)

Abstract The need for incorporating the traction-free condition at the air-earth boundary for finite-difference modeling of seismic wave propagation has been discussed widely. A new implementation has been developed for simulating elastic wave propagation in which the free-surface condition is replaced by an explicit acoustic-elastic boundary. Detailed comparisons of seismograms with different implementations for the air-earth boundary were undertaken using the (2,2) (the finite-difference operators are second order in time and space) and the (2,6) (second order in time and sixth order in space) standard staggered-grid (SSG) schemes. Methods used in these comparisons to define the air-earth boundary included the stress image method (SIM), the heterogeneous approach, the scheme of modifying material properties based on transversely isotropic medium approach, the acoustic-elastic boundary approach, and an analytical approach. The method proposed achieves the same or higher accuracy of modeled body waves relative to the SIM. Rayleigh waves calculated using the explicit acoustic-elastic boundary approach differ slightly from those calculated using the SIM. Numerical results indicate that when using the (2,2) SSG scheme for SIM and our new method, a spatial step of 16 points per minimum wavelength is sufficient to achieve 90% accuracy; 32 points per minimum wavelength achieves 95% accuracy in modeled Rayleigh waves. When using the (2,6) SSG scheme for the two methods, a spatial step of eight points per minimum wavelength achieves 95% accuracy in modeled Rayleigh waves. Our proposed method is physically reasonable and, based on dispersive analysis of simulated seismographs from a layered half-space model, is highly accurate. As a bonus, our proposed method is easy to program and slightly faster than the SIM.
Plasma cortisol, androstenedione, testosterone and luteinizing hormone in running exercise of different intensities (1980)

Changes in plasma cortisol, androstenedione, testosterone and luteinizing hormone (LH) were measured in five young male sprinters after maximal short-term running and in five young male long-distance runners after moderate (90 min, 4.3 min/km) and intense (45 min, 3.3 min/km) long-term running. Short-term running increased mean plasma cortisol (27%) and androstenedione (19%) significantly; no appreciable changes were found in mean plasma testosterone or LH levels. Intense long-term running caused considerable increases in mean plasma cortisol (43%) and androstenedione (53%). Immediately after the long-term runs mean plasma testosterone and LH did not show nay significant changes, but half an hour later mean plasma LH was found to have dropped significantly below the preceding level, by 42% after the moderate run and by 45% after the intense run. At this time the intense long-term run caused a significant decrease in mean plasma testosterone, which remained depressed up to 3 h after the end of the exercise. This study implies that the changes in plasma hormone levels depend more on the intensity of the exercise than on its duration. The activity of the pituitary-adrenocortical system appears to be a good indicator of the effort expended during the exercise. In the pituitary-testicular system, in contrast, the effort expended may be more accurately reflected by changes during the recovery period.
Dynamics of Major Histocompatibility Complex Class I Association with the Human Peptide-loading Complex (2012)

Although the human peptide-loading complex (PLC) is required for optimal major histocompatibility complex class I (MHC I) antigen presentation, its composition is still incompletely understood. The ratio of the transporter associated with antigen processing (TAP) and MHC I to tapasin, which is responsible for MHC I recruitment and peptide binding optimization, is particularly critical for modeling of the PLC. Here, we characterized the stoichiometry of the human PLC using both biophysical and biochemical approaches. By means of single-molecule pulldown (SiMPull), we determined a TAP/tapasin ratio of 1:2, consistent with previous studies of insect-cell microsomes, rat-human chimeric cells, and HeLa cells expressing truncated TAP subunits. We also report that the tapasin/MHC I ratio varies, with the PLC population comprising both 2:1 and 2:2 complexes, based on mutational and co-precipitation studies. The MHC I-saturated PLC may be particularly prevalent among peptide-selective alleles, such as HLA-C4. Additionally, MHC I association with the PLC increases when its peptide supply is reduced by inhibiting the proteasome or by blocking TAP-mediated peptide transport using viral inhibitors. Taken together, our results indicate that the composition of the human PLC varies under normal conditions and dynamically adapts to alterations in peptide supply that may arise during viral infection. These findings improve our understanding of the quality control of MHC I peptide loading and may aid the structural and functional modeling of the human PLC.
BECAS DE LA CONTAINER CORPORATION (1960)

LA CONTAINER CORPORATION OF AMERICA A RENOVADO LAS BECAS DE 500.00 DOLARES POR SEIS MESES, A LOS ALUMNOS DE LA CARRERA DE DIBUJANTE PUBLICITARIO DE LA ESCUELA NACIONAL DE ARTE PLASTICAS DE LA UNIVERSIDAD NACIONAL AUTONOMA DE MEXICO. LOS ALUMNOS FERNANDO REYES TODD Y CARLOS URIBE GARCIA LA CONTINUARAN RECIBIENDO DURANTE ESTE LAPSO DE TIEMPO Y SIN OBLIGACION QUE HACER ALGUNOS TRABAJOS CON IDEAS ORIGINALES PARA LA CITADA EMPRESA.
Evidence for an Essential Carboxyl Group for Yeast Phosphoglycerate Kinase (1974)

Abstract An alternative method of purifying 3-phosphoglycerate kinase (EC 2.7.2.3) from yeast is described which employs step-induced pH-gradient elution from phosphocellulose and electrofocusing. The purified enzyme has a specific activity of 1200 to 1400 units per mg when corrected to saturating conditions and 30°. Reaction of this enzyme with Woodward's reagent K. (N-ethyl-5-phenylisoxazolium-3'-sulfonate) leads to rapid inhibition; MgATP affords protection against inactivation. Inhibition obeys pseudo-first order kinetics and there is an enzyme inhibitor complex formed prior to covalent modification. Although up to 5 moles of carboxyl per mole of phosphoglycerate kinase can be modified by Woodward's reagent K, it appears that only a single carboxyl is essential for the enzyme's action. The essential carboxyl appears to be more reactive than the nonessential but modifiable carboxyls. Sedimentation velocity and optical rotatory dispersion studies indicate that there has been no major conformational change concomitant with modification. Possible mechanistic roles for the essential carboxyl are discussed.