gebhart/patrepeval huggingface dataset:
DETECTION OF VIABLE <i>LISTERIA MONOCYTOGENES</i> IN MILK USING AN ELECTROCHEMICAL METHOD (2003)

Abstract Growth of Listeria monocytogenes in a Listeria enrichment broth (LEB) was automatically monitored by electrochemical cyclic voltammetric scan using a gold working electrode. Changes in cyclic voltammograms were observed during growth of L. monocytogenes in LEB. The reduction peak at −0.4 V (vs Ag/AgCl) corresponding to the reduction of oxygen dissolved in LEB on cyclic voltammograms was decreasing with proliferation of L. monocytogenes, and disappeared eventually. A pair of reversible redox peaks appeared during growth of L. monocytogenes in LEB. These cyclic voltammetric characteristics can be used to detect L. monocytogenes in various samples. Threshold values (detection time) obtained from the oxygen consumption curves were inversely related to the concentrations of L. monocytogenes in the broth. A calibration curve was obtained by plotting initial cell concentrations (CFU/mL) determined by conventional plate counting, as a function of the detection time. A linear response was found on the calibration curve for L. monocytogenes between 1 ∼ 9 times 10 0 and 1 ∼ 9 times 10 5 cells/mL. The detection time was approximately 17 and 6 h for 1 ∼ 9 times 10 0 and 1 ∼ 9 × 10 5 cells/mL of viable L. monocytogenes in the broth, respectively. The method was evaluated in detection of L. monocytogenes in milk samples.
EVIDENCE FOR RECYCLING OF SYNAPTIC VESICLE MEMBRANE DURING TRANSMITTER RELEASE AT THE FROG NEUROMUSCULAR JUNCTION (1973)

When the nerves of isolated frog sartorius muscles were stimulated at 10 Hz, synaptic vesicles in the motor nerve terminals became transiently depleted. This depletion apparently resulted from a redistribution rather than disappearance of synaptic vesicle membrane, since the total amount of membrane comprising these nerve terminals remained constant during stimulation. At 1 min of stimulation, the 30% depletion in synaptic vesicle membrane was nearly balanced by an increase in plasma membrane, suggesting that vesicle membrane rapidly moved to the surface as it might if vesicles released their content of transmitter by exocytosis. After 15 min of stimulation, the 60% depletion of synaptic vesicle membrane was largely balanced by the appearance of numerous irregular membrane-walled cisternae inside the terminals, suggesting that vesicle membrane was retrieved from the surface as cisternae. When muscles were rested after 15 min of stimulation, cisternae disappeared and synaptic vesicles reappeared, suggesting that cisternae divided to form new synaptic vesicles so that the original vesicle membrane was now recycled into new synaptic vesicles. When muscles were soaked in horseradish peroxidase (HRP), this tracerfirst entered the cisternae which formed during stimulation and then entered a large proportion of the synaptic vesicles which reappeared during rest, strengthening the idea that synaptic vesicle membrane added to the surface was retrieved as cisternae which subsequently divided to form new vesicles. When muscles containing HRP in synaptic vesicles were washed to remove extracellular HRP and restimulated, HRP disappeared from vesicles without appearing in the new cisternae formed during the second stimulation, confirming that a one-way recycling of synaptic membrane, from the surface through cisternae to new vesicles, was occurring. Coated vesicles apparently represented the actual mechanism for retrieval of synaptic vesicle membrane from the plasma membrane, because during nerve stimulation they proliferated at regions of the nerve terminals covered by Schwann processes, took up peroxidase, and appeared in various stages of coalescence with cisternae. In contrast, synaptic vesicles did not appear to return directly from the surface to form cisternae, and cisternae themselves never appeared directly connected to the surface. Thus, during stimulation the intracellular compartments of this synapse change shape and take up extracellular protein in a manner which indicates that synaptic vesicle membrane added to the surface during exocytosis is retrieved by coated vesicles and recycled into new synaptic vesicles by way of intermediate cisternae.
Pressure?volume measurements by conductance catheter during cardiac resynchronization therapy (2004)

Pressure-volume measurements by conductance catheter enable assessment of systolic and diastolic left ventricular function and quantification of mechanical dyssynchrony in a continuous and on-line fashion. The present review gives an overview of the (potential) applications of this methodology in relation to cardiac resynchronization therapy.
Deletion analysis of the mouse m1 muscarinic acetylcholine receptor: effects on phosphoinositide metabolism and down-regulation (1989)

Deletions have been constructed in the putative third cytoplasmic loop of the mouse m1 muscarinic acetylcholine receptor (mAChR) gene, and the effects of these mutations on mAChR coupling to phosphoinositide metabolism and agonist-induced down-regulation have been examined following expression in Y1 adrenal carcinoma cells. Deletion of up to 123 of the 156 amino acids in this loop has no effect on antagonist or agonist binding, or on coupling to stimulation of phosphoinositide metabolism. These results suggest that the membrane proximal portions of this loop are involved in determining the specificity of functional coupling of the receptor. Deletion of 75% of the loop has no effect on short-term agonist-induced internalization but does cause a significant decrease in the magnitude of agonist-induced down-regulation of receptor number. Thus, this portion of the receptor may be involved in mediating the response to long-term agonist exposure.
A Low-Profile Three-Dimensional Silicon/Parylene Stimulating Electrode Array for Neural Prosthesis Applications (2005)

This paper describes a low-profile three-dimensional silicon/parylene microelectrode array as basis for practical neural prostheses for use in the central nervous system. The circuit areas of the silicon probes, containing mixed-signal CMOS circuitry for neural stimulation/recording, can be folded over to reduce the overall height of the microassembled array above the cortical surface. The low- profile structure is implemented using multiple gold beams spaced by orthogonal silicon braces. An integrated silicon/parylene batch process is introduced to encapsulate these interconnects and achieve high yield.
Ultradrawing of amorphous polymers by coextrusion illustrated with atactic polystyrene (1979)

Abstract Films of pure and high‐impact atactic polystyrene were prepared by the recently developed technique of solid‐state coextrusion. The films were produced at extrusion rates ≥4 cm/min at 126°C with a maximum extrusion draw ratio (EDR) of 11.6. These ultradrawn films are fibrous, have a high birefringence of −2.24 × 10 −2 , and exhibit a 72% elastic recovery. The material has a tensile modulus of ∼4–5 GPa and a tensile strength to break of 85 MPa. Thermal analysis suggests a constant T g .
The table widget reloaded (2010)

Many applications present data sets that are essentially large tables. Certain features of such table views have become commonplace, and a comprehensive set of interactions with tables could easily be available in a platform-independent way in the form of a widget. Table widgets are not a new concept, but previous examples have some shortcomings in functionality and nonfunctional aspects. They are not explicitly based on a platform-independent set of functional requirements and do not take into account the types of data presented. Those table widgets that are part of the GUI framework do not take into account nonfunctional requirements such as the size of the data set. This paper presents the requirements for a universal table widget, and describes a prototype implementation that fulfills many of these requirements. Its features include the ability to handle large data sets, heterogeneous row and column sizes and heterogeneous content types.
Cystatin C and the Risk of Death and Cardiovascular Events among Elderly Persons (2005)

Cystatin C, a serum measure of renal function, is a stronger predictor of the risk of death and cardiovascular events in elderly persons than is creatinine.
A new soft-switched contactless battery charger with robust local controllers (2003)

Abstract- A new soft-switched contactless battery charger with an improved local controller is described. The controller consists of a primary-current feedback control circuit in the inverter side and an output-voltage feedback control circuit in the rectifier side. By controlling the primary current of the inverter side, the maximum transferable power through the inductive coupling is automatically kept constant over the entire input voltage and load ranges. As a result, excessive circulating energy in the charger is minimized. Moreover, by incorporating the proposed controller, zero-voltage switching (ZVS) of all power switches is achieved. Finally, the proposed controller provides a tight regulation of the output voltage over the entire load and input voltage ranges without any feedback connection between the rectifier side and the inverter side. 1.
Generation of mouse polyclonal and human monoclonal antibodies against Bacillus anthracis toxin. (2005)

High titer antisera against the protective antigen (PA) from Bacillus anthracis were generated immunizing Balb/c mice two times intraperitoneally with PA in combination with lipopeptide adjuvant P3CSK4. The sera were able to protect the mouse macrophage cell line J774A.1 from an anthrax toxin challenge. We also tested the blood of anthrax vaccine-immunized persons for PA- and lethal factor (LF)-specific antibodies. An increased titer was found after three immunizations, and the sera were also able to protect the mouse macrophage cell line from a toxin challenge. For the preparation of human monoclonal antibodies, we used peripheral blood lymphocytes. After in vitro stimulation using PA or synthetic peptides derived from PA, B lymphocytes were immortalized by PEG fusion with the human mouse heteromyeloma cell line CB-F7. We obtained several clones producing high amounts of PA-specific immunoglobulin (Ig).
De longs ARN non codants activateurs de la transcription des gènes (2011)

International audience
Cloning and Disruption of CKB1, the Gene Encoding the 38-kDa β Subunit of Saccharomyces cerevisiae Casein Kinase II (CKII) (1995)

Saccharomyces cerevisiae casein kinase II (CKII) contains two distinct catalytic (alpha and alpha') and regulatory (beta and beta') subunits. We report here the isolation and disruption of the gene, CKB1, encoding the 38-kDa beta subunit. The predicted Ckb1 sequence includes the N-terminal autophosphorylation site, internal acidic domain, and potential metal binding motif (CPX3C-X22-CPXC) present in other beta subunits but is unique in that it contains two additional autophosphorylation sites as well as a 30-amino-acid acidic insert. CKB1 is located on the left arm of chromosome VII, approximately 33 kilobases from the centromere and does not correspond to any previously characterized genetic locus. Haploid and diploid strains lacking either or both beta subunit genes are viable, demonstrating that the regulatory subunit of CKII is dispensable in S. cerevisiae. Such strains exhibit wild type behavior with regard to growth on both fermentable and nonfermentable carbon sources, mating, sporulation, spore germination, and resistance to heatshock and nitrogen starvation, but are salt-sensitive. Salt sensitivity is specific for NaCl and LiCl and is not observed with KCl or agents which increase osmotic pressure alone. These data suggest a role for CKII in ion homeostasis in S. cerevisiae.
A Comparative Analysis of the Plant Cellulose Synthase (<i>CesA</i>) Gene Family (2000)

CesA genes are believed to encode the catalytic subunit of cellulose synthase. Identification of nine distinct CesA cDNAs from maize (Zea mays) has allowed us to initiate comparative studies with homologs from Arabidopsis and other plant species. Mapping studies show that closely related CesA genes are not clustered but are found at different chromosomal locations in both Arabidopsis and maize. Furthermore, sequence comparisons among the CesA-deduced proteins show that these cluster in groups wherein orthologs are often more similar than paralogs, indicating that different subclasses evolved prior to the divergence of the monocot and dicot lineages. Studies using reverse transcriptase polymerase chain reaction with gene-specific primers for six of the nine maize genes indicate that all genes are expressed to at least some level in all of the organs examined. However, when expression patterns for a few selected genes from maize and Arabidopsis were analyzed in more detail, they were found to be expressed in unique cell types engaged in either primary or secondary wall synthesis. These studies also indicate that amino acid sequence comparisons, at least in some cases, may have value for prediction of such patterns of gene expression. Such analyses begin to provide insights useful for future genetic engineering of cellulose deposition, in that identification of close orthologs across species may prove useful for prediction of patterns of gene expression and may also aid in prediction of mutant combinations that may be necessary to generate severe phenotypes.
Performance enhancement of composition-graded-base type-II InP∕GaAsSb double-heterojunction bipolar transistors with fT&gt;500GHz (2006)

The high frequency performance of type-II InP∕GaAsSb double heterojunction bipolar transistors (DHBTs) with compositionally graded and uniform 25nm base layers and 65nm InP collectors is investigated. The graded base DHBT achieves a peak current gain of 20.5 compared to 14.9 for the uniform base DHBT at current densities near 15mA∕μm2. The peak fT of a graded base device with 0.38×8μm2 emitter dimensions improves from 505GHz at 25°C to 535GHz at −55°C as determined by −20dB∕decade extrapolation. The base grading has reduced the total transit time by 25% compared to the uniform base type-II DHBT.
SIVELESTAT (SELECTIVE NEUTROPHIL ELASTASE INHIBITOR) IMPROVES THE MORTALITY RATE OF SEPSIS ASSOCIATED WITH BOTH ACUTE RESPIRATORY DISTRESS SYNDROME AND DISSEMINATED INTRAVASCULAR COAGULATION PATIENTS (2009)

Neutrophil elastase plays an important role in the development of acute respiratory distress syndrome (ARDS) and disseminated intravascular coagulation (DIC) in sepsis. Sivelestat is a selective neutrophil elastase inhibitor. It is possible that sivelestat improves the outcome of septic patients associated with ARDS and DIC. A retrospective data analysis of septic patients associated with ARDS and DIC was conducted to investigate the effects of sivelestat. Observational period was 5 days after admission to intensive care unit (ICU). The study included 167 septic patients associated with ARDS and DIC. Control group included 133 patients without sivelestat, and sivelestat group included 34 patients started to deadministered sivelestat on the admission to ICU. The lung injury scores and Pa(O2)/Fl(O2) ratio of the sivelestat group were significantly more severe than those of the control group from days 1 to 4. On day 5, the lung injury score and Pa(O2)/Fl(O2) ratio of the sivelestat group improved to the same levels of those of the control group. The DIC score of sivelestat group improved on day 3 in comparison to day 1, and those of control group remained unchanged until day 4. The length of ICU stay of the sivelestat group was significantly shorter than that of the control group. A stepwise multiple logistic-regression analysis showed the sivelestat administration to be an independent predictor of survival of the septic patients associated with both ARDS and DIC. The length of ICU stay of the sivelestat group was significantly shorter than that of the control group. In addition, sivelestat administration was found to be an independent predictor of survival of those patients.
On understanding the nature of slow fading in LOS microcellular channels (2002)

Our investigations in understanding the physical mechanism of the LOS microcellular channel reveal that the principle source of slow fading is the random interference pattern of a few strong contributing paths which causes what we term as deceptive shadowing. It is shown that in a comparative curve fitting of slow fading, Nakagami distribution also fits the fading data. Also in non-zero mean complex Gaussian channels, the segregation of slow and fast fading using moving mean normalization is illusionary as the fading parameters in both cases remain the same. We argue that radio link performance calculation based on the segregation of slow and fast fading are therefore unnecessary for Rice/lognormal or Nakagami/lognormal fading models and may be avoided in order to simplify complex analyses.
Ford, Henry: My Life and Work (2020)

Das 1922 erschienene Buch, das von dem Wirtschaftsjournalisten Samuel Crowther verfasst wurde, beruht auf Gesprächen mit Ford sowie Informationen der Presseabteilung der Firma Ford und ist deshalb mit Vorbehalten zu lesen: Der Werdegang des jungen Ford wird in der Erinnerung mitunter verklärt, der Prototyp seines ersten Fahrzeugs falsch auf 1893 datiert – möglicherweise absichtlich zwecks Harmonisierung des Buches mit einem von 1903 bis 1911 laufenden Patentstreit, bei dem Ford dieses Baujahr behauptete.
Theory of polyampholyte solutions (1991)

We consider polyampholyte polymers containing both positive and negative monomers randomly dispersed along the chain. Neutral chains collapse into a globule due to attractive electrostatic interactions. The behavior of the charges inside the globule is similar to that of charges in a small volume of simple electrolyte. A screening length κ−1p coming from the polymeric charge may be defined as in Debye–Hückel theory. The internal structure of the globule is that of close packed blobs of radius equal to the screening length. When salt is added this further screens the interactions and reduces the attractions. The globule begins to increase in size when the concentration of salt becomes larger than the concentration of charge on the polymer itself. Screened Coulomb interactions in a neutral chain behave like a negative contribution to excluded volume. For a chain in a good solvent there is a θ salt concentration at which the net excluded volume becomes zero. Chains are swollen above this concentration of salt, and collapsed below this concentration. For small sections of chain the Coulomb interactions are unscreened and cannot be treated as a modification to excluded volume. Chains with a strong net charge of one sign tend to behave as conventional polyelectrolyte with charges of only one sign. We determine the criterion for the value of the net charge at which the repulsions (polyelectrolyte effect) begin to dominate the attractions (polyampholyte effect). The predictions are found to be in good qualitative agreement with experiments.
Development of Solid Pesticide Formulations by Fluidized-Bed Technology (1988)

The fluidized bed granulator is versatile. Its application to pesticide formulation includes granulation, drying, coating and mixing products. In a fluidized bed granulation process, the granulation and drying are concurrent process in the same unit. It offers several advantages over the most common process, pan granulation method, in making water dispersible granules. The fluidized bed granulation can also be used to convert a liquid formulation containing a surfactant into a solid product. Some water soluble pesticides are the examples of this application.
On zonotopes (1971)

In this paper is described a diagram technique for zonotopes, or vector sums of line segments, which is analogous to that of Gale diagrams for general polytopes, and central diagrams for centrally symmetric polytopes. The use of these new zonal diagrams leads to relationships between zonotopes with <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="n"> <mml:semantics> <mml:mi>n</mml:mi> <mml:annotation encoding="application/x-tex">n</mml:annotation> </mml:semantics> </mml:math> </inline-formula> zones of dimensions <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="d"> <mml:semantics> <mml:mi>d</mml:mi> <mml:annotation encoding="application/x-tex">d</mml:annotation> </mml:semantics> </mml:math> </inline-formula> and <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="n minus d"> <mml:semantics> <mml:mrow> <mml:mi>n</mml:mi> <mml:mo>−</mml:mo> <mml:mi>d</mml:mi> </mml:mrow> <mml:annotation encoding="application/x-tex">n - d</mml:annotation> </mml:semantics> </mml:math> </inline-formula>, and enables one to enumerate all the combinatorial types of <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="d"> <mml:semantics> <mml:mi>d</mml:mi> <mml:annotation encoding="application/x-tex">d</mml:annotation> </mml:semantics> </mml:math> </inline-formula>-zonotopes with <inline-formula content-type="math/mathml"> <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" alttext="n less-than-over-equals d plus 2"> <mml:semantics> <mml:mrow> <mml:mi>n</mml:mi> <mml:mo>≦</mml:mo> <mml:mi>d</mml:mi> <mml:mo>+</mml:mo> <mml:mn>2</mml:mn> </mml:mrow> <mml:annotation encoding="application/x-tex">n \leqq d + 2</mml:annotation> </mml:semantics> </mml:math> </inline-formula> zones. The connexion between arrangements of hyperplanes in projective space and zonotopes leads to corresponding new results about arrangements.
In the I of the Storm: The Problems With Pluralism (1993)

On Friday, 11 September 1992, at 5:30 a.m. Hawaii time, Civil Defense sirens alerted Hawaii residents to impending disaster—Hurricane Iniki was headed directly for us. Turn on your radios the blare of the siren insisted, identify today's particular danger and prepare to batten down the hatches. A class-four hurricane, Iniki's weapons included sustained winds of 165 miles per hour and the memory of Hurricane Andrew not far from our minds. Though it will take years to recover from the loss of life, property, and employment wreaked by these two hurricanes, the power of a hurricane dissipates relatively quickly. The infrastructure of American society works rapidly through agencies such as FEMA and the Red Cross. While there is incredible damage to external manifestations of the structure (houses, hotels, worksites), the structure itself remains unchanged. On Friday, 31 July 1992, at 1:00 p.m. Atlanta time, Lynda Hart sounded an equally important siren. As she closed her presentation on the Looking at Seeing Panel of the 1992 Women and Theatre Conference, she cited a recent Operation Rescue attack on an abortion clinic in New York where sidewalk counselors for the right to life were saying Abortion is the new holocaust. The only people who should be going in there are Blacks and Jews. As the conference participants gasped in horror, Hart asked us, How much more evidence do we need to see that race and gender and class can't be theorized discretely? (10). As we tuned in more closely for details of this impending disaster and how to prepare for it, she implored us to look carefully, to remember that every looking has to be a looking again, a constant process of remembering what we've forgotten, and forgetting what we remember. The crisis in feminist theory that Hart identified—the crisis more devastating and certainly more sustained than the fury of a hurricane—is the exclusive location of gender discrete from issues of race, class, and sexuality as the defining element of feminist inquiry. This is not a danger against which we, as feminists, can fortify ourselves and then emerge