gebhart/patrepeval huggingface dataset:
Bactericidal Activity of Photocatalytic TiO <sub>2</sub> Reaction: toward an Understanding of Its Killing Mechanism (1999)

When titanium dioxide (TiO(2)) is irradiated with near-UV light, this semiconductor exhibits strong bactericidal activity. In this paper, we present the first evidence that the lipid peroxidation reaction is the underlying mechanism of death of Escherichia coli K-12 cells that are irradiated in the presence of the TiO(2) photocatalyst. Using production of malondialdehyde (MDA) as an index to assess cell membrane damage by lipid peroxidation, we observed that there was an exponential increase in the production of MDA, whose concentration reached 1.1 to 2.4 nmol. mg (dry weight) of cells(-1) after 30 min of illumination, and that the kinetics of this process paralleled cell death. Under these conditions, concomitant losses of 77 to 93% of the cell respiratory activity were also detected, as measured by both oxygen uptake and reduction of 2,3,5-triphenyltetrazolium chloride from succinate as the electron donor. The occurrence of lipid peroxidation and the simultaneous losses of both membrane-dependent respiratory activity and cell viability depended strictly on the presence of both light and TiO(2). We concluded that TiO(2) photocatalysis promoted peroxidation of the polyunsaturated phospholipid component of the lipid membrane initially and induced major disorder in the E. coli cell membrane. Subsequently, essential functions that rely on intact cell membrane architecture, such as respiratory activity, were lost, and cell death was inevitable.
Isolation and characterization of cDNA clones for carrot extensin and a proline-rich 33-kDa protein (1985)

Extensins are hydroxyproline-rich glycoproteins associated with most dicotyledonous plant cell walls. To isolate cDNA clones encoding extensin, we started by isolating poly(A)(+) RNA from carrot root tissue, and then translating the RNA in vitro, in the presence of tritiated leucine or proline. A 33-kDa peptide was identified in the translation products as a putative extensin precursor because: (i) it is rich in proline and poor in leucine, and (ii) the message appears to be more abundant when carrot tissue is wounded. From a cDNA library constructed with poly(A)(+) RNA from wounded carrots, one cDNA clone (pDC5) was identified that specifically hybridized to poly(A)(+) RNA encoding this 33-kDa peptide. We isolated three cDNA clones (pDC11, pDC12, and pDC16) from another cDNA library using pDC5 as a probe. DNA sequence data, RNA hybridization analysis, and hybrid released in vitro translation indicate that the cDNA clone pDC11 encodes extensin and that cDNA clones pDC12 and pDC16 encode the 33-kDa peptide, which as yet has an unknown identity and function. The assumption that the 33-kDa peptide was an extensin precursor was invalid. RNA hybridization and DNA sequence analysis indicate that pDC5 is a hybrid clone corresponding to two RNA species. RNA hybridization analysis showed that RNA encoded by both clone types is accumulated upon wounding.
Key-Management Infrastructure for Protecting Stored Data (2008)

Any secure use of encryption requires good key management. Historically, it has been hard to do key management well and there has been almost no interoperability between key-management products from different vendors. But help is on the way, at least for some of the most important cases. A new standard from the IEEE P1619 Security in Storage Working Group (SISWG) will make it easier to manage the keys used to encrypt data in storage. This standard will greatly simplify key management and finally make interoperable key management possible. Products that implement the standard should be available by next year. Encryption has traditionally been both expensive and difficult to use. This limited its widespread use to little more than SSL, the protocol that's used to authenticate Web servers and encrypt connections to them. The recent proliferation of data security and privacy laws, however, has made the use of encryption attractive in many other cases. On the one hand, it's fairly easy to convince auditors that sensitive data is being protected adequately when it's encrypted. On the other hand, because encryption is actually one of the best ways to protect sensitive data, it's much more than just a check box that will keep auditors happy.
Conservative Management of Metatarsal and Heel Pain in the Adult Foot (1987)

The Louisiana State University (LSU) Affiliated Hospitals foot clinic has had good success in the treatment of metatarsal and heel pain using the following five modalities. Muscle strengthening and muscle stretching of the intrinsic muscles and the muscles about the ankle joint, especially the gastrocnemius-soleus complex. Contrast baths in the acute condition to decrease the edema and inflammatory process. Nonsteroidal agents with the acute condition to decrease the inflammatory response. Shoe modifications. Orthotic devices. Orthotic devices seem to be the most useful in the treatment of both conditions. It has been found necessary to prescribe orthotics in almost all cases of chronic problems to institute permanent relief. Opposition remains to multiple steroid injections into either area, although there is no great objection to one or possibly two injections into a particular area to decrease the inflammatory process. It has been observed that a general worsening of the condition occurs from multiple injections. After repeated steroid injections one sets the stage for increased atrophy of the fat pad, which results in a more prominent bony surface and increased pain. Cases have been documented on many occasions showing indented areas over the heel or metatarsals or complete loss of the metatarsal fat pad of patients who have received multiple injections of steroids.
Folding of ω-Conotoxins.  1. Efficient Disulfide-Coupled Folding of Mature Sequences in Vitro (1996)

Disulfide-coupled refolding reactions of five omega-conotoxins, Ca2+ channel antagonists derived from marine snails of the genus Conus, were examined. These peptides are 23-26 amino acid residues long, and the native conformation of each is stabilized by three disulfide bonds. Although the primary structures of the peptides show only limited sequence similarity, the patterns of disulfides and three-dimensional conformations are very similar. Refolding of the reduced proteins was promoted by the disulfide form of glutathione (GSSG) in the presence of reduced glutathione (GSH). All five of the peptides examined were able to refold to the native conformation, as judged by reversed-phase HPLC behavior, with efficiencies of 16% for omega-MVIIC, 28% for omega-MVIID, and 50% for omega-MVIIA, omega-GVIA, and omega-SVIA. The refolded form of omega-MVIIA was further shown to have biological activity indistinguishable from that of the native form, as well as the same rate of reductive unfolding in the presence of dithiothreitol. The overall folding rate and efficiency of omega-MVIIA was found to be quite sensitive to the thiol-disulfide redox potential, with optimum rates and yields obtained in the presence of GSSG and GSH at concentrations similar to those believed to be present in the endoplasmic reticulum. The folding efficiency of this peptide was greatly reduced by the addition of 8 M urea, indicating that formation of the correct disulfides is determined largely by noncovalent interactions, as opposed to steric constraints arising from the spacing between Cys residues. These results demonstrate that the mature forms of at least some omega-conotoxins contain sufficient information to direct correct folding and disulfide formation, in spite of their small size and limited sequence conservation.
La reproducción artificial humana frente al derecho civil colombiano. Parte 1 (2016)

Articulo publicado en dos entregas. Para consultar la primera parte dirijase al N° 86, esta entrega contiene: III. La problematica juridica colombiana de la reproduccion humana asistida – A. El contexto del problema – B. ?Contrato de donacion seminal o contrato de venta de semen? – C. Contrato de prestamo de vientres o maternidad delegada – D. Contrato de inseminacion artificial – E. Sobre las tecnicas de fertilizacion asistida y la responsabilidad civil. – F. Acerca del derecho de la personalidad sobre el cuerpo – G. Acerca del matrimonio civil
Direct Acylation of C(sp<sup>3</sup>)−H Bonds Enabled by Nickel and Photoredox Catalysis (2016)

Using nickel and photoredox catalysis, the direct functionalization of C(sp(3))-H bonds of N-aryl amines by acyl electrophiles is described. The method affords a diverse range of α-amino ketones at room temperature and is amenable to late-stage coupling of complex and biologically relevant groups. C(sp(3))-H activation occurs by photoredox-mediated oxidation to generate α-amino radicals which are intercepted by nickel in catalytic C(sp(3))-C coupling. The merger of these two modes of catalysis leverages nickel's unique properties in alkyl cross-coupling while avoiding limitations commonly associated with transition-metal-mediated C(sp(3))-H activation, including requirements for chelating directing groups and high reaction temperatures.
Development of a Conceptual Framework toward an Integrated Transportation System (2009)

This report documents research on the conceptual framework of an integrated transportation system with a prototype application under the framework. Three levels of control are involved in this framework: at the global level (an entire transportation system), at the local level (a highway segment, an intersection, or a freeway merge), and at the vehicle level (a vehicle and its surrounding). The prototype application is a Vehicle Infrastructure Integration (VII)-enabled intersection collision warning system.
Long-term (56-week) oral administration of probiotic Enterococcus faecium M-74 decreases the expression of sICAM-1 and monocyte CD54, and increases that of lymphocyte CD49d in humans. (2005)

We observed significant changes in CAMs expression in terms of a decrease in sICAM-1, CD54 on monocytes and CD11b on lymphocytes after one-year administration of Enterococcus faecium M-74 in humans. Anti-adhesion-aimed therapeutic modalities may provide the future approach to prevention and treatment of cardiovascular diseases. Application of probiotics may be part of such strategies. (Tab. 2, Fig. 6, Ref. 41.)
Studies on Condensed Pyrimidine Systems. XVII. Some Halogenopurines (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Condensed Pyrimidine Systems. XVII. Some HalogenopurinesGertrude B. Elion and George H. HitchingsCite this: J. Am. Chem. Soc. 1956, 78, 14, 3508–3510Publication Date (Print):July 1, 1956Publication History Published online1 May 2002Published inissue 1 July 1956https://pubs.acs.org/doi/10.1021/ja01595a065https://doi.org/10.1021/ja01595a065research-articleACS PublicationsRequest reuse permissionsArticle Views751Altmetric-Citations46LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Activation of retinoic acid receptor signaling coordinates lineage commitment of spontaneously differentiating mouse embryonic stem cells in embryoid bodies (2010)

Retinoid signaling has been implicated in embryonic stem cell differentiation. Here we present a systematic analysis of gene expression changes in mouse embryonic stem cells (mESCs), during their spontaneous differentiation into embryoid bodies and the effect of all-trans retinoic acid (ATRA) on this process. We show that retinoic acid is present in the serum and is sufficient to activate retinoid signaling at a basal level in undifferentiated mESCs. This signal disappears during embryoid body formation. However exogenously added ATRA resets the spontaneous differentiation programs in embryoid bodies and initiates a distinct genetic program. These data suggest that retinoid signaling not only promotes a particular pathway but also acts as a context dependent general coordinator of the differentiation states in embryonic stem cells.
Kinect v2 for mobile robot navigation: Evaluation and modeling (2015)

With the introduction of the Microsoft Kinect for Windows v2 (Kinect v2), an exciting new sensor is available to robotics and computer vision researchers. Similar to the original Kinect, the sensor is capable of acquiring accurate depth images at high rates. This is useful for robot navigation as dense and robust maps of the environment can be created. Opposed to the original Kinect working with the structured light technology, the Kinect v2 is based on the time-of-flight measurement principle and might also be used outdoors in sunlight. In this paper, we evaluate the application of the Kinect v2 depth sensor for mobile robot navigation. The results of calibrating the intrinsic camera parameters are presented and the minimal range of the depth sensor is examined. We analyze the data quality of the measurements for indoors and outdoors in overcast and direct sunlight situations. To this end, we introduce empirically derived noise models for the Kinect v2 sensor in both axial and lateral directions. The noise models take the measurement distance, the angle of the observed surface, and the sunlight incidence angle into account. These models can be used in post-processing to filter the Kinect v2 depth images for a variety of applications.
The incidence of acute cardiorespiratory and vascular dysfunction following intramedullary nail fixation of femoral metastasis (2000)

Intramedullary nail fixation is a common treatment for metastatic tumors of the femur with overt or impending femoral fracture. This procedure sometimes causes severe cardiorespiratory and vascular dysfunction. The clinical relevance of this is not dear. We reviewed 45 operations in 43 patients, where intramedullary nail fixation was used to treat metastatic femoral fractures and impending fractures. We studied the incidence of intraoperative oxygen desaturation and hypotension associated with intramedullary manipulation as markers of cardiorespiratory and vascular dysfunction. Acute oxygen desaturation and hypotension occurred in 11 of our 45 patients. Of these, 3 died, 2 required intensive care postoperatively and 6 made uneventful recoveries. We hope to highlight a serious complication in this patient group.
Radiobiology and Clinical Application of Halogenated Pyrimidine Radiosensitizers (1989)

Halogenated pyrimidines (HP) represent a unique class of non-hypoxic cell radiosensitizers currently under clinical re-investigation. In order for halogenated pyrimidines to sensitize cells to radiation, they must be incorporated into cellular DNA. In the case of human tumors, which have in general rather long cell cycle times, this may require many days of continuous drug infusion to achieve adequate replacement of the DNA base thymidine with HP. In vitro studies support the relationship between the extent of radiosensitization and the percentage of thymidine replacement. Recent clinical studies evaluating the role of iododeoxyuridine (IdUrd) as a radiation sensitizer in large unresectable sarcomas have been extremely encouraging. To support and expand upon these positive clinical findings more information and research is needed regarding: (1) the mechanism of HP-induced radiosensitization; (2) the percentage of HP thymidine replacement in human tumors achievable and how it relates to treatment outcome; (3) the means of increasing HP incorporation in tumor and minimizing incorporation in normal tissues; (4) a better understanding of optimal timing between HP administration and radiation treatment; and (5) methods to evaluate which tumors are appropriate candidates for HP therapy. While presently limited to use in conventional high dose-rate X-ray therapy, laboratory studies suggest that HP might also be effective in low dose-rate brachytherapy and for selected high LET clinical beams. HPs probably will not be 'general' non-hypoxic cell radiosensitizers for all tumor types, but with appropriate tumor-type/anatomical site selection and refinement in their administration, HPs may prove beneficial in cancer treatment.
Effect of gefitinib on EGFR Mutated Lung Cancer Cell Lines (2005)

3172 Background: EGFR is frequently overexpressed in many cancerous tumors such as lung, colon, breast and brain. Receptor tyrosine kinase inhibitors (TKI) such as gefitinib (Iressa) and Tarceva have been widely used for targeted therapy in cancer. These drugs inhibit the EGFR kinase activity by binding the ATP pocket within the catalytic domain. The inhibition of EGFR blocks MAP Kinase and PI3K-Akt pathways, and therefore, preventing cell proliferation and tumor growth. Recent clinical studies of patients with NSCLC showed that Tarceva is most effective in population with EGFR mutation. We describe a multiplexed proximity-based eTag assays for EGFR and Her family receptor dimerization following signaling phosphorylations, to study the effect Iressa on EGFR inhibition in lung cancer cell lines with mutant EGFR. Material and Methods: Lung cancer cell lines such as NCI-358 and NCI-1734 expressing wild type EGFR and NCI-1650 expressing mutant EGFR (in-frame deletion delE746-A750) and NCI-1975 expressing EGFR with missense mutation L858R were selected. These cell lines were treated with Iressa for 1–16 h followed by 100 ng/ml of EGF stimulation. EGFR/Her receptors were analyzed for receptor dimerization and downstream signaling pathway activation. Results: Her1/1 and Her1/2 were detected in both wild type and mutant cell cell lines. Upon treatment with Iressa, the EGFR phosphoryltion was inhibited in both wild type and mutant lung cancer cell lines, but the IC50s for cell lines with mutant EGFR were ∼50–100 times lower. Her1/1 and Her1/2 dimerization significantly increased in all cell lines following stimulation with Iressa. The MAP kinase pathway was inhibited upon treatment with Iressa, and like EGFR inhibition, the IC50s for mutant cell lines were 50–100 times lower as compared to wild type cell lines. Conclusion: We used the multiplexed eTag Assay system to study the effect of Iressa on EGFR in model cell lines, which express wild type and mutant EGFR. The results indicated that these EGFR inhibitors are more effective in cells with mutant EGFR. No significant financial relationships to disclose.
Power control requirements in linear decorrelating detectors for CDMA (2002)

This paper addresses the effects of synchronisation errors (time delay, carrier phase, and carrier frequency) on the performance of linear decorrelating detectors (LDDs). A major effect is that all LDDs require certain degree of power control in the presence of synchronisation errors. The multi-shot sliding window algorithm (SLWA) and hard decision method (HDM) are analysed and their power control requirements are examined. Also, a more efficient one-shot detection scheme, called "hard-decision based coupling cancellation", is proposed and analysed. These schemes are then compared with the isolation bit insertion (IBI) approach in terms of power control requirements.
Anti-Phospholipase A2 Receptor Antibody Titer Predicts Post-Rituximab Outcome of Membranous Nephropathy (2015)

Rituximab induces nephrotic syndrome (NS) remission in two-thirds of patients with primary membranous nephropathy (MN), even after other treatments have failed. To assess the relationships among treatment effect, circulating nephritogenic anti-phospholipase A2 receptor (anti-PLA2R) autoantibodies and genetic polymorphisms predisposing to antibody production we serially monitored 24-hour proteinuria and antibody titer in patients with primary MN and long-lasting NS consenting to rituximab (375 mg/m(2)) therapy and genetic analyses. Over a median (range) follow-up of 30.8 (6.0-145.4) months, 84 of 132 rituximab-treated patients achieved complete or partial NS remission (primary end point), and 25 relapsed after remission. Outcomes of patients with or without detectable anti-PLA2R antibodies at baseline were similar. Among the 81 patients with antibodies, lower anti-PLA2R antibody titer at baseline (P=0.001) and full antibody depletion 6 months post-rituximab (hazard ratio [HR], 7.90; 95% confidence interval [95% CI], 2.54 to 24.60; P<0.001) strongly predicted remission. All 25 complete remissions were preceded by complete anti-PLA2R antibody depletion. On average, 50% anti-PLA2R titer reduction preceded equivalent proteinuria reduction by 10 months. Re-emergence of circulating antibodies predicted disease relapse (HR, 6.54; 95% CI, 1.57 to 27.40; P=0.01), whereas initial complete remission protected from the event (HR, 6.63; 95% CI, 2.37 to 18.53; P<0.001). Eighteen patients achieved persistent antibody depletion and complete remission and never relapsed. Outcome was independent of PLA2R1 and HLA-DQA1 polymorphisms and of previous immunosuppressive treatment. Therefore, assessing circulating anti-PLA2R autoantibodies and proteinuria may help in monitoring disease activity and guiding personalized rituximab therapy in nephrotic patients with primary MN.
NASA Robotic Neurosurgery Testbed (1997)

The detection of tissue interface (e.g., normal tissue, cancer, tumor) has been limited clinically to tactile feedback, temperature monitoring, and the use of a miniature ultrasound probe for tissue differentiation during surgical operations, In neurosurgery, the needle used in the standard stereotactic CT or MRI guided brain biopsy provides no information about the tissue being sampled. The tissue sampled depends entirely upon the accuracy with which the localization provided by the preoperative CT or MRI scan is translated to the intracranial biopsy site. In addition, no information about the tissue being traversed by the needle (e.g., a blood vessel) is provided. Hemorrhage due to the biopsy needle tearing a blood vessel within the brain is the most devastating complication of stereotactic CT/MRI guided brain biopsy. A robotic neurosurgery testbed has been developed at NASA Ames Research Center as a spin-off of technologies from space, aeronautics and medical programs. The invention entitled Robotic Neurosurgery Leading to Multimodality Devices for Tissue Identification is nearing a state ready for commercialization. The devices will: 1) improve diagnostic accuracy and precision of general surgery, with near term emphasis on stereotactic brain biopsy, 2) automate tissue identification, with near term emphasis on stereotactic brain biopsy, to permit remote control of the procedure, and 3) reduce morbidity for stereotactic brain biopsy. The commercial impact from this work is the potential development of a whole new generation of smart surgical tools to increase the safety, accuracy and efficiency of surgical procedures. Other potential markets include smart surgical tools for tumor ablation in neurosurgery, general exploratory surgery, prostate cancer surgery, and breast cancer surgery.