gebhart/patrepeval huggingface dataset:
In Operando X-ray Absorption Fine Structure Studies of Polyoxometalate Molecular Cluster Batteries: Polyoxometalates as Electron Sponges (2012)

We carried out in operando Mo K-edge X-ray absorption fine structure measurements on the rechargeable molecular cluster batteries (MCBs) of polyoxometalates (POMs), in which a Keggin-type POM, [PMo(12)O(40)](3-), is utilized as a cathode active material with a lithium metal anode. The POM-MCBs exhibit a large capacity of ca. 270 (A h)/kg in a voltage range between V = 4.0 V and V = 1.5 V. X-ray absorption near-edge structure analyses demonstrate that all 12 Mo(6+) ions in [PMo(12)O(40)](3-) are reduced to Mo(4+) in the discharging process. This means the formation of a super-reduced state of the POM, namely, [PMo(12)O(40)](27-), which stores 24 electrons, and this electron number can explain the large capacity of the POM-MCBs. Furthermore, extended X-ray absorption fine structure analyses reveal the molecular structure of [PMo(12)O(40)](27-), which is slightly reduced in size compared to the original [PMo(12)O(40)](3-) and involves Mo(4+) metal-metal-bonded triangles. Density functional theory calculations suggest that these triangles are formed because of the large number of additional electrons in the super-reduced state.
Attenuation of acute hypoxic pulmonary vasoconstriction and hypoxic pulmonary hypertension in mice by inhibition of Rho-kinase (2004)

RhoA GTPase mediates a variety of cellular responses, including activation of the contractile apparatus, growth, and gene expression. Acute hypoxia activates RhoA and, in turn, its downstream effector, Rho-kinase, and previous studies in rats have suggested a role for Rho/Rho-kinase signaling in both acute and chronically hypoxic pulmonary vasoconstriction. We therefore hypothesized that activation of Rho/Rho-kinase in the pulmonary circulation of mice contributes to acute hypoxic pulmonary vasoconstriction and chronic hypoxia-induced pulmonary hypertension and vascular remodeling. In isolated, salt solution-perfused mouse lungs, acute administration of the Rho-kinase inhibitor Y-27632 (1 x 10(-5) M) attenuated hypoxic vasoconstriction as well as that due to angiotensin II and KCl. Chronic treatment with Y-27632 (30 mg x kg(-1) x day(-1)) via subcutaneous osmotic pump decreased right ventricular systolic pressure, right ventricular hypertrophy, and neomuscularization of the distal pulmonary vasculature in mice exposed to hypobaric hypoxia for 14 days. Analysis of a small number of proximal pulmonary arteries suggested that Y-27632 treatment reduced the level of phospho-CPI-17, a Rho-kinase target, in hypoxic lungs. We also found that endothelial nitric oxide synthase protein in hypoxic lungs was augmented by Y-27632, suggesting that enhanced nitric oxide production might have played a role in the Y-27632-induced attenuation of chronically hypoxic pulmonary hypertension. In conclusion, Rho/Rho-kinase activation is important in the effects of both acute and chronic hypoxia on the pulmonary circulation of mice, possibly by contributing to both vasoconstriction and vascular remodeling.
Scalable high speed IP routing lookups (1997)

Internet address lookup is a challenging problem because of increasing routing table sizes, increased traffic, higher speed links, and the migration to 128 bit IPv6 addresses. IP routing lookup requires computing the best matching prefix, for which standard solutions like hashing were believed to be inapplicable. The best existing solution we know of, BSD radix tries, scales badly as IP moves to 128 bit addresses. Our paper describes a new algorithm for best matching prefix using binary search on hash tables organized by prefix lengths. Our scheme scales very well as address and routing table sizes increase: independent of the table size, it requires a worst case time of log 2 ( address bits ) hash lookups. Thus only 5 hash lookups are needed for IPv4 and 7 for IPv6. We also introduce Mutating Binary Search and other optimizations that, for a typical IPv4 backbone router with over 33,000 entries, considerably reduce the average number of hashes to less than 2, of which one hash can be simplified to an indexed array access. We expect similar average case behavior for IPv6.
Interface Influence on the Proton-Conductivity of Ordered Mesoporous Silica Membranes (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
An Improved General Procedure for the Synthesis of N,N' ‐ Bisacrylyldiaminoalkanes (1980)

Abstract Recently our laboratory has attempted the synthesis of a beaded poly (dimethylacrylamide) support for use in solid phase peptide synthesis. The preparation of this resin requires the ready availability of N,N'-bisacrylyl-1,2-diaminoethane. To date the only literature preparation of this monomer(1) requires very cold temperatures, ion exchange chromatography, and several recrystallizations resulting in a moderate overall yield. We wish to report here a simple and fast general procedure for the synthesis of N,N'-bisacrylyldiamino-alkanes which results in high yields of pure products.
생약의 류코트리엔 B4 수용체결합 저해작용 검색 (2000)

Leukotriene B₄ (LTB₄) is a pro-inflammatory mediator synthesized in myeloid cells from arachidonic acid. Elevated levels of LTB₄ have been found in a number of inflammatory diseases and levels are related to disease activity in some of these. Because LTB₄ interacts with cells through specific cell surface receptors, LTB₄ receptor blockade is the most specific approach to reduce the pathogenic role of LTB₄. In order to find LTB₄ receptor antagonist from plants, we screened the LTB₄ receptor antagonistic activity of the methanol extract and solvent fractions of herbal drugs. The ability of samples to inhibit specific binding of [³H]-LTB₄ to human peripheral neutrophils was used as assay to evaluate the antagonistic activity of plant materials. Among the tested methanol extracts of herbal drugs, Mori Radicis Cortex, Perillae Semen, Armeniacae Semen and Sophorae subprostratae Radix showed potent inhibitory activity above 70% at the concentration of 100 ㎍/㎖. The inhibitory activities of LTB₄ binding to human neutrophils were evaluated for several solvent fractions at three different concentrations. Especially, hexane soluble fractions of Anemarrhenae Rhizoma and Embeliae Radix, and ethyl acetate soluble fractions of Aristolochiae Fructus, Magnoliae Cortex and Zingiberis Rhizoma crudus shwed moderate activity at 25 ㎍/㎖. These fractions were promising candidates for the study of the activity-guided chromatographic purification of active compounds. Silica gel column chromatography of hexane soluble fractions of Anemarrhenae Rhizoma and Embeliae Radix gave very active sub-fractions, AA-4 and ES-4, and their inhibition activities of LTB₄ binding to human neutrophil at 30 ㎍/㎖ were 78% and 62%, respectively. From these results we could anticipate new LTB₄ receptor antagonist from herbal drugs, and the block of LTB₄ effects may provide beneficial in neutrophil mediated diseases such as inflammation and bronchial asthma.
Genomic Analyses of the Microsporidian Nosema ceranae, an Emergent Pathogen of Honey Bees (2009)

Recent steep declines in honey bee health have severely impacted the beekeeping industry, presenting new risks for agricultural commodities that depend on insect pollination. Honey bee declines could reflect increased pressures from parasites and pathogens. The incidence of the microsporidian pathogen Nosema ceranae has increased significantly in the past decade. Here we present a draft assembly (7.86 MB) of the N. ceranae genome derived from pyrosequence data, including initial gene models and genomic comparisons with other members of this highly derived fungal lineage. N. ceranae has a strongly AT-biased genome (74% A+T) and a diversity of repetitive elements, complicating the assembly. Of 2,614 predicted protein-coding sequences, we conservatively estimate that 1,366 have homologs in the microsporidian Encephalitozoon cuniculi, the most closely related published genome sequence. We identify genes conserved among microsporidia that lack clear homology outside this group, which are of special interest as potential virulence factors in this group of obligate parasites. A substantial fraction of the diminutive N. ceranae proteome consists of novel and transposable-element proteins. For a majority of well-supported gene models, a conserved sense-strand motif can be found within 15 bases upstream of the start codon; a previously uncharacterized version of this motif is also present in E. cuniculi. These comparisons provide insight into the architecture, regulation, and evolution of microsporidian genomes, and will drive investigations into honey bee-Nosema interactions.
Effect of zeta potentials of oil droplets and bubbles on flotation of oil‐in‐water mixtures (1988)

Abstract The effects of surface charges of oil droplets and bubbles in the oil‐in‐water flotation process were studied by a zeta potential apparatus which was specially designed to measure the zeta potential of bubbles. To measure the electrophoretic velocity of bubbles accurately, small bubbles whose rising velocities were less than 1 mm/s were introduced into an electrophoresis cell by a method of pressure reduction of a solution containing dissolved air, and the movement of the bubble in the cell was followed by moving the cell vertically at the same velocity as that of the rising bubble. It was found that the separation efficiency, n , for the flotation was strongly dependent on the zeta potentials of both the oil droplets and the bubbles. n increased as the zeta potentials of both the oil droplets and the bubbles decreased. Also n became the largest when the oil droplets were negatively charged and the bubbles were positively charged. Furthermore, a critical adhesion parameter, m =4πϵζ p ζ b /kA, between oil droplets and bubbles was calculated by the heterocoagulation theory, using the value of the experimentally determined Hamaker constant, A =3.38x 10 −20 J, and the dependence of n on m was examined.
Up-regulation of HIV coreceptors CXCR4 and CCR5 on CD4+ T cells during human endotoxemia and after stimulation with (myco)bacterial antigens: the role of cytokines (2000)

Concurrent infections in patients with human immunodeficiency virus (HIV) infection stimulate HIV replication. Chemokine receptors CXCR4 and CCR5 can act as HIV coreceptors. The authors hypothesized that concurrent infection increases the HIV load through up-regulation of CXCR4 and CCR5. Using experimental endotoxemia as a model of infection, changes in HIV coreceptor expression were assessed in 8 subjects injected with lipopolysaccharide (LPS, 4 ng/kg). The expression of CXCR4 and CCR5 on CD4(+) T cells was increased 2- to 4-fold, 4 to 6 hours after LPS injection. In whole blood in vitro, LPS induced a time- and dose-dependent increase in the expression of CXCR4 and CCR5 on CD4(+) T cells. Similar changes were observed after stimulation with cell wall components of Mycobacterium tuberculosis (lipoarabinnomannan) or Staphylococcus aureus (lipoteichoic acid), or with staphylococcal enterotoxin B. LPS increased viral infectivity of CD4-enriched peripheral blood mononuclear cells (PBMCs) with a T-tropic HIV strain. In contrast, M-tropic virus infectivity was reduced, possibly because of elevated levels of the CCR5 ligand cytokines RANTES and MIP-1beta. LPS-stimulated up-regulation of CXCR4 and CCR5 in vitro was inhibited by anti-TNF and anti-IFN gamma. Incubation with recombinant TNF or IFN gamma mimicked the LPS effect. Anti-interleukin 10 (anti-IL-10) reduced CCR5 expression, without influencing CXCR4. In accordance, rIL-10 induced up-regulation of CCR5, but not of CXCR4. Intercurrent infections during HIV infection may up-regulate CXCR4 and CCR5 on CD4(+) T cells, at least in part via the action of cytokines. Such infections may favor selectivity of HIV for CD4(+) T cells expressing CXCR4. (Blood. 2000;96:2649-2654)
Vertex covers and connected vertex covers in 3-connected graphs (1991)

Discusses time complexity analysis of the minimum vertex cover and minimum connected vertex cover problems for 3-connected graphs. A vertex cover of a graph G=(V, E) is a subset N of V such that each element of E is incident upon some element of N, where V and E are the sets of vertices and of edges of G, respectively. A connected vertex cover of a graph G is a vertex cover of G such that the subgraph G(N) induced by N of G is connected. The minimum vertex cover problem (VCP) is the problem of finding a vertex cover of minimum cardinality, and the minimum connected vertex cover problem is similarly defined.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Folates and prevention of disease (2001)

Research in the past decade has established that low or inadequate folate status may contribute to congenital malformations and the development of chronic disease in later life. Using an evidence based approach, there are clear guidelines for recommending folic acid supplementation or fortification in certain disease conditions but further proof of its efficacy is required in other circumstances. There is conclusive evidence that maternal periconceptional supplementation with folic acid prevents the majority of NTDs, probably by overcoming one or more genetically inherited metabolic blocks in folate dependent enzymes. Public health efforts to advise women to increase their folate intake have not been successful. As a result, the U.S. government passed legislation to have all flour fortified with folic acid. This intervention has had a dramatic effect on folate status in the U.S. To date, countries of the EU have not adopted mandatory fortification policies. The amino acid homocysteine is an essential intermediate in folate metabolism. Substantial evidence indicates that elevated plasma homocysteine is an independent risk factor for heart disease and stroke. Plasma homocysteine levels can be reduced by folic acid supplements. A food fortification policy would probably be an effective population strategy to reduce plasma homocysteine. However, many experts believe that this would be premature without first showing that such reduction would cause a decrease in the prevalence of cardiovascular disease. The contribution of folate to cancer risk is not well defined although there is reasonable evidence to implicate low folate status in the specific case of colorectal cancer. In particular, long-term folic acid supplementation may reduce risk of colorectal cancer substantially. Various mental disorders including Alzheimer's Disease have been associated with low folate status or elevated plasma homocysteine. While it is hard to determine if this is cause or effect, there is little doubt that if it were true then low dose folic acid intervention would be highly effective.
Organization of histaminergic fibers in the rat brain (1988)

Detailed information on innervation of the histaminergic system in the brain is essential to an understanding of the physiological roles of this system. In a previous immunocytochemical study with antihistidine decarboxylase (HDC) antibody, we detected extensive networks of histaminergic fibers in many areas of the rat brain (Watanabe et al., '84). In the present study, we improved the immunocytochemical procedure and examined the detailed distribution of histaminergic innervation in the rat brain with anti-HDC antibody. As reported previously, the highest concentrations of fibers were found in the hypothalamic nuclei and medial forebrain bundle. With the modified procedure, we detected more dense networks of HDC-immunoreactive (HDCI) fibers. Furthermore, we demonstrated for the first time the existence of HDCI fibers in other regions, namely, the thalamic nuclei, median eminence, fimbria of the hippocampus, habenular nuclei, superior colliculus, nucleus of the optic tract, parabrachial nuclei, mesencephalic trigeminal nucleus, superior, lateral, and spinal vestibular nuclei, posterior lobe of the hypophysis, and vascular organ of the lamina terminalis. We also found dense transverse fibers in the retrochiasmatic area and supraoptic decussation, which suggests bilateral innervation of the histaminergic system. These results indicate that innervation of the rat brain by the histaminergic system is more extensive than observed previously.
Hydrogen-terminated Si Surfaces. Formation of Hydrogen Terminated Silicon Surfaces. (1999)

Recently, a convenient wet-chemical treatment method to terminate silicon surface with hydrogen atoms has been developed. It is attractive from the viewpoint of surface science and device technology. However, since the atomic structure and surface microroughness of the surfaces treated by the wet-chemical process is strongly affected by the condition, we should optimize the treatment condition in order to make atomicallyflat and well-ordered hydrogen-terminated surface. Here we discuss the optimization of wetchemical treatment of the hydrogen-terminated Si(111) and (001) surfaces.
Comparison of the nucleic acids of helical and coccoid forms of Helicobacter pylori (1997)

The nucleic acids of the helical and coccoid forms of Helicobacter pylori were studied to determine if the coccoid forms are "viable (capable of growing) but nonculturable." Using a reference strain (NCTC 11638) and five clinical strains, the nucleic acid contents, DNA integrity, and results of PCR and reverse transcription-PCR (RT-PCR) were compared for helical H. pylori and coccoid forms induced using glycochenodeoxycholic acid or bismuth citrate. The DNA and RNA contents of the coccoid forms were respectively 6.8- and 8.1-fold lower than those of helical H. pylori after 3 days of induction and 11.5- and 14.7-fold lower after 7 days. Agarose gel electrophoresis of DNA extracted from the coccoid forms after 3 days of induction showed a smear pattern indicating DNA cleavage, whereas DNA from helical H. pylori showed a single band with a high molecular mass. After 12 days of induction, all RNA samples from 100% coccoid cultures were negative for the mRNA of urease A or the 26-kDa species-specific protein by RT-PCR. However, most RNA samples obtained after 3 or 7 days of induction were positive at low levels despite the lack of recovery from these cultures. These results suggest that the coccoid form of H. pylori has impaired genomic DNA and is in the process of cellular degeneration, thus being still alive but nonincreasable.
Characteristics of Tissue‐Engineered Cartilage on Macroporous Biodegradable PLGA Scaffold (2006)

The new tissue-engineered cartilage in vivo on PLGA scaffolds displayed the biochemical characteristics of cartilage tissue, and it showed chondrocyte-specific phenotypes and morphology that were similar to the native cartilage.
Modulation of bronchial epithelial cell barrier function by in vitro ozone exposure. (1994)

The epithelial cells lining the small, peripheral airways function as important targets for the action of inspired ozone. Loss of epithelial barrier integrity in these regions is a common element in ozone-induced airway inflammation. To investigate the direct effect of ozone on epithelial barrier function, canine bronchial epithelial (CBE) cells grown with an air interface were exposed for 3 hr to 0.2, 0.5, or 0.8 ppm ozone or to air. Mannitol flux, used as an index of paracellular permeability, increased above air controls by 461%, 774%, and 1172% at the three ozone concentrations, respectively. Transcellular electrical resistance exhibited a dose-related decrease. The immediate effect of 0.8 ppm ozone on permeability was significantly inhibited by preincubation for 48 hr in the presence of 1 ng/ml vitamin E (33%) or 1 microM vitamin A (34%). Responses to 0.5 ppm or 0.8 ppm were inhibited by pretreatment of the cells with 0.1 microM of the actin polymerizing agent phalloidin (34% and 25% inhibition, respectively). The increases in permeability induced by 0.2 and 0.5 ppm ozone were attenuated by 54% and 22%, respectively, at 18 hr after exposure, whereas that to 0.8 ppm was further enhanced by 42% at this time. The effects of ozone are modulated by the availability of antioxidants to the cells and appear to be associated with cytoskeletal dysfunction in CBE cells. The data are consistent with a loss of barrier function linked to a direct oxidative effect of ozone on individual CBE cells and indicate that the reversible or progressive nature of this effect is dose dependent.
Studies on immunological paralysis. X. Cellular characteristics of the induction and loss of tolerance to levan (Polyfructose). (1972)

The induction and loss of tolerance to native and depolymerized fractions of levan, a thymus-independent antigen, have been studied in (DBA/1×CBA-T6T6)F(1) mice by a direct plaque-forming cell (PFC) assay. Tolerance following 1 mg native levan began to break by 80 days, whereas mice remained specifically unresponsive 150 days after 10 mg. Effects attributable to long persistence of antigen have been demonstrated. The following main features of levan tolerance were established by transfer of spleen cells to 900 R-irradiated recipients: (1) the induction period is very short—less than 24 hours. (2) No detectable immunity accompanies or precedes induction. (3) The fall in immunogenicity with reduction in molecular weight (mol. wt) of levan is not accompanied by loss of tolerogenicity. An effectively tolerogenic, non-immunogenic fraction (mol. wt<10,000) was found. (4) There is no rapid loss of tolerance following cell transfer (reactivity is only regained after 50 days), arguing against the existence of tolerant cells in this model. As these results and those from corresponding investigations with SIII and HGG all show major dissimilarities, it is considered imprudent to base generalizations concerning the nature of B cell tolerance on studies with only one type of antigen. Whether or not two different kinds of specific unresponsiveness might be implicated with these cells is discussed.
On an Unbroken Alternating Current for Cable Telegraphy (1914)

The Paper proposes a new angle of view in the method of transmission of signals in the submarine telegraph cable, and describes some apparatus for operating on the general principles involved. An ocean cable is considered as a power line, and starting with the standard form of circuit which would be used in case it were required to operate an electric motor through an ocean cable, experiments are described to determine the minimum possible variations required in such a circuit to permit the alternating current received to be interpreted in dots, dashes, and spaces of the present alphabet. The uninterrupted alternating current used in transmission is operated on synchronously by the ordinary transmitting tape, so as to alter the impedance of the transmitting circuit at the instants when the current is naturally zero. Dots, dashes and spaces are each sent by semi-waves of either sign, but of different amplitudes. The alternating current received may be read directly from the record made by a siphon recorder, or this current may be employed to operate a siphon Morse printer, by means of an adaptation of Muirhead's gold-wire relay, or a Heurtley magnifier and a local wire relay. The voltage stress along an Atlantic cable when an alternator is employed is shown, and the transmitting impedance of such a cable is computed as the frequency varies. A special form of cable dynamo to operate at frequencies from 4 to 10 was used in the experiments described. The fundamental principle is developed of never metallically breaking the transmitter circuit, which permits of greater accuracy in balancing the duplex bridge.