gebhart/patrepeval huggingface dataset:
Combinatorial peptide ligand libraries for urine proteome analysis: Investigation of different elution systems (2009)

Proteome treatments with peptide libraries in view of reducing high-abundance proteins and increasing the concentration of rare species involve the adsorption on solid-phase material. Subsequent elution of captured proteins may not be fully effective except when sequences of eluting agents are used. The standard way utilized up to the present has been a three- to four-step, sequential elution system consisting of various agents mixed together such as urea, thiourea, CHAPS, sodium chloride, citric or acetic acid and some polar solvents such as ACN and isopropanol. Elution sequences produce distinct fractions adding to the burden of having to analyze all of them. An alternative, highly effective, single elution to reduce the workload is here reported for the first time, namely elution in boiling 10% SDS added with 3% DTE. This single step elutes almost quantitatively the adsorbed proteins, thus ensuring, for all practical purposes, a full recovery. This high efficiency is believed to be due to the fact that the SDS micelles bury the polypeptide chains within their hydrophobic core, thus shielding them from the surroundings and impeding accidental adsorption to surfaces. Suggestions for selecting the best method to eliminate the excess of SDS for further protein analysis are also evaluated. The merits and limits of this novel system are assessed and discussed.
Molecular cloning of a chloride channel that is regulated by dehydration and expressed predominantly in kidney medulla. (1994)

The nucleotide sequence
Central Role of CD45RA− Foxp3hi Memory Regulatory T Cells in Clinical Kidney Transplantation Tolerance (2015)

The role of Foxp3(+) regulatory T cells (Tregs) in operational tolerance remains elusive, as initial results revealed an increased frequency of this subset in tolerant patients but no functional differences compared with immunosuppressed recipients. In addition, recent studies of regulatory B cells strongly suggest that Tregs may not have a central role in kidney transplantation tolerance. However, recent investigations of the crucial role of Foxp3 demethylation in Treg function and the possibility of identifying distinct Foxp3 T cell subsets prompted us to more thoroughly characterize Tregs in operationally tolerant patients. Thus, we studied the level of demethylation of the Foxp3 Treg-specific demethylated region (TSDR) in circulating CD4(+) T cells and analyzed Treg subset frequency in tolerant patients, healthy volunteers, patients with stable graft function under immunosuppression, and chronically rejecting recipients. We observed a higher proportion of CD4(+) T cells with demethylated Foxp3 and a specific expansion of CD4(+) CD45RA(-) Foxp3(hi) memory Tregs exclusively in tolerant patients. The memory Tregs of tolerant recipients exhibited increased Foxp3 TSDR demethylation, expressed higher levels of CD39 and glucocorticoid-induced TNF-related receptor, and harbored greater suppressive properties than memory Tregs from patients with stable graft function. Taken together, our data demonstrate that operationally tolerant patients mobilize an array of potentially suppressive cells, including not only regulatory B cells but also Tregs. Our results also indicate that tolerant patients have potent CD4(+)CD45RA(-) Foxp3(hi) memory Tregs with a specific Foxp3 TSDR demethylation pattern, which may contribute to the maintenance of graft tolerance.
Looking for the best immune‐checkpoint inhibitor in pre‐treated NSCLC patients: An indirect comparison between nivolumab, pembrolizumab and atezolizumab (2017)

Immune-checkpoint inhibitors represent the new standard of care in patients with advanced NSCLC who progressed after first-line treatment. This work aim to assess any difference in both efficacy and safety profiles among Nivolumab, Pembrolizumab and Atezolizumab in pre-treated NSCLC patients. Randomized clinical trials comparing immune-checkpoint inhibitor versus docetaxel in pre-treated patients with advanced NSCLC were included and direct comparison meta-analysis of selected trials have been performed. Subsequently the summary estimates of Nivolumab, Pembrolizumab and Atezolizumab emerging from the direct meta-analysis were selected to provide the pooled estimates of hazard ratio (HR) and relative risk (RR) for the indirect comparisons among these agents. A total of 5 studies met the selection criteria and were included in the meta-analysis. Indirect comparisons for efficacy outcomes showed the RR for ORR nivolumab versus atezolizumab 1.66 (95% CI 1.07-2.58), pembrolizumab versus atezolizumab 1.94 (95% CI 1.30-2.90). No significant differences in both PFS and OS have been observed. Indirect comparisons for safety showed the RR for G3-5 AEs nivolumab versus pembrolizumab 0.41 (95% CI 0.29-0.60), nivolumab versus atezolizumab 0.50 (95% CI 0.35-0.72). No significant differences in both pneumonitis and discontinuation rate have been observed. The results of this work revealed that nivolumab and pembrolizumab are associated with a significant increase of ORR as compared to atezolizumab and nivolumab is associated with a significant lower incidence of G3-5 AEs as compared to the other drugs. These evidences could support the oncologists to select the best drug for each patient.
Data Collection of Freeway Travel Time Ground Truth with Bluetooth Sensors (2010)

Accurate travel time information is essential to the effective management of traffic conditions. Traditionally, floating car data have been used as the primary source of ground truth for measuring the quality of real-time travel time provided by traffic surveillance systems. This paper introduces Bluetooth sensors as a new and effective means of data collection of freeway ground truth travel time. The concept of vehicle identification using Bluetooth signatures for travel time estimation along a section of freeway is explained. Issues related to error analysis, filtering of raw matched data, and accuracy of the resulting ground truth compared with floating car are discussed. Data from loop detectors on several freeway segments are used to approximate and report the average sampling rate of Bluetooth sensors. Results show that the new technology is a promising method for collecting high-quality travel time data that can be used as ground truth for evaluating other sources of travel time and other intelligent transportation system applications.
DC-Bus Voltage Range Extension in 1500 V Photovoltaic Inverters (2015)

Solar plants based on single-stage conversion photovoltaic (PV) inverters (no dc-dc boost stage) have gained popularity due to their simplicity, high efficiency, and cost effectiveness. Existing PV plants mostly operate under 1000 V and are subject to wide dc-bus voltage variations due to the effect of PV cell temperature and the voltage of the maximum power point (well below the open-circuit voltage). In particular, attractive locations, such as U.S.-Canada mountain and central regions, are subject to extreme PV surface temperatures, making the dc-bus voltage variation a challenge for single-stage solar inverters. This paper investigates 1500 V solar inverters with a focus on dc-bus voltage range extension capabilities through novel modulation and power devices utilization. A comparative analysis with the existing 1000 V solar inverters is presented to illustrate the significant advantages of the wide dc-bus range in 1500 V systems. A lower voltage limit in the dc-bus is achieved by employing a novel voltage reactive power dc-bus control strategy and modified modulation. A higher dc-bus peak voltage is obtained by maximizing the use of power switches. As a result, the 1500 V inverter dc-bus voltage is significantly extended to capture energy under extreme PV surface temperatures, greatly improving the limited range of traditional 1000 V inverters. Simulations and experimental results using a three-phase three-level neutral point-clamped inverter level are presented to validate the proposed dc-bus extension range, control strategy, and modulation scheme.
NMOS Ring oscillators with cobalt-silicided P-diffused shallow junctions formed during the "Poly-plug" contact doping cycle (1986)

Composite silicided source-drains are being developed to provide low-resistance shallow junctions for high performance fine-line circuits. The junctions are usually formed either by implantation and drive prior to silicide formation or else by implantation immediately after, followed by a heat cycle. This paper describes a novel approach for the fabrication of CoSi <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> /n <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+</sup> -p junctions (2 . 5 Ω/□ sheet resistance), wherein the junctions are doped by diffusion through the contact windows using the conventional "poly-plug" doping cycle [1], [2]. LPCVD poly-Si is deposited on windows to previously silicided gate and source-drain regions, and exposed to PBr <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</inf> at an elevated temperature. Since the diffusivity of dopants in silicides is higher than in bulk Si, this step transports the P through the poly-Si via the windows laterally into the silicide, to form uniformly doped junction surrounds. This poly-Si doping scheme for junction fabrication eliminates an ion-implant step, provides an independent means of tailoring channel length, and can potentially result in low-resistance contacts even if the window etch step has punched through the silicide, Electrical characteristics of 1.25-µm gate-length ring oscillators are similar to those of circuits processed with the conventional As implant and drive. Transistor <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">I-V</tex> 's and subthreshold behavior remain unaffected by the silicide doping process. Junction depth and leakage are sensitive functions of the poly-plug thermal cycle, with a 950°C 30-min drive resulting in 0.3-µm junctions. For a 1-µm design rule circuit layout, 30 to 45 min at 950°C is judged adequate.
Visible and invisible (1992)

In The City of God Augustine develops two related ideas. The Church includes all the elect, those who have already died, those still living and those yet to be born, in a bond of unity which transcends the ties of this life. It is therefore a community whose invisible reality is only imperfectly and partly embodied in its visible presence on earth. Secondly, because the human community on earth includes members of both ‘cities’, and because (as Augustine held) only God knows who are his elect, the ostensible Church membership here and now is in fact a mixed community. Wheat and tares grow together until harvest. The principle is echoed in Thomas Becon, Anglican reformer and Homilist, in his Catechism; he points out that ‘This word “Church” in the Holy Scripture is taken not only for the fellowship or company of the good and faithful, but also of the wicked and unfaithful.’ Augustine saw it as essential that the earthly community should preserve its unity as one body, saints and sinners together; no-one should presume to have special knowledge which enabled him to say who were God's people and who were not. There was thus a built-in tension in Augustine's ecclesiology. Visible and invisible Churches have different constitutions.
N–H Stretching Excitations in Adenosine-Thymidine Base Pairs in Solution: Pair Geometries, Infrared Line Shapes, and Ultrafast Vibrational Dynamics (2012)

We explore the N-H stretching vibrations of adenosine-thymidine base pairs in chloroform solution with linear and nonlinear infrared spectroscopy. Based on estimates from NMR measurements and ab initio calculations, we conclude that adenosine and thymidine form hydrogen bonded base pairs in Watson-Crick, reverse Watson-Crick, Hoogsteen, and reverse Hoogsteen configurations with similar probability. Steady-state concentration and temperature dependent linear FT-IR studies, including H/D exchange experiments, reveal that these hydrogen-bonded base pairs have complex N-H/N-D stretching spectra with a multitude of spectral components. Nonlinear 2D-IR spectroscopic results, together with IR-pump-IR-probe measurements, as also corroborated by ab initio calculations, reveal that the number of N-H stretching transitions is larger than the total number of N-H stretching modes. This is explained by couplings to other modes, such as an underdamped low-frequency hydrogen-bond mode, and a Fermi resonance with NH(2) bending overtone levels of the adenosine amino-group. Our results demonstrate that modeling based on local N-H stretching vibrations only is not sufficient and call for further refinement of the description of the N-H stretching manifolds of nucleic acid base pairs of adenosine and thymidine, incorporating a multitude of couplings with fingerprint and low-frequency modes.
Internet Banking Login with Multi-Factor Authentication (2016)

Internet banking is one of many services provided by financial institutions that have become very popular with an increasing trend. Due to the increased amount of usage of the service, Internet banking has become a target from adversaries. One of the points that are at risk of an attack is the login process. Therefore, it is necessary to have a security mechanism that can reduce this risk. This research designs and develops a multi-factor authentication protocol, starting from a registration system, which generates authentication factors, to an actual authentication mechanism. These factors can be categorised into two groups: short term and long term. For the authentication protocol, only three messages need to be exchanged between a client and a financial institution's server. Many cryptographic processes are incorporated into the protocol, such as symmetric and asymmetric cryptography, a symmetric key generation process, a method for generating and verifying digital signatures. All of the authentication messages have been proved and analysed by the logic of GNY and the criteria of OWASP-AT-009. Even though there are additional factors of authentication, users do not really feel any extra load on their part, as shown by the satisfactory survey.
Effect of Interfacial Debonding on the Strength of Composite Structure-Similarity Scale Model for the Wing-Box (2015)

Based on the wing-box structure, a model was established to analyze the strength of the scale model for the composite wing. Firstly, different failure criteria were set to determine damage onset of the components. The continuum damage variables were adopted in the stiffness degradation rule. Secondly, the interface elements were placed along the interface between the beam flange and the skin to investigate the effects of bonding strength on the ultimate load-carrying capacity of the wing-box. The failure modes of the wing-box structure were studied by using the nonlinear finite element method. The effect of flange’s width on the strength of wing-box was discussed based on the prediction method. The results indicated that the ultimate load-carrying capacity varied distinctly with the change of flange’s width. However, the bonding strength had limited effect on the model strength as the flange’s width increases to the critical value. The research methods and results of the study can serve as reference for the strength analysis on the scale model of composite wing as well as the determination of principles adopted in the design of the scale model for wing spar.
Highly Efficient Conversion of Ammonia in Electricity by Solid Oxide Fuel Cells (2006)

Hydrogen is the fuel for fuel cells with the highest cell voltage. A drawback for the use of hydrogen is the low energy density storage capacity, even at high pressures. Liquid fuels such as gasoline and methanol have a high energy density but lead to the emission of the greenhouse gas CO2. Ammonia could be the ideal bridge fuel, having a high energy density at relative low pressure and no (local) CO2 emission. Ammonia as a fuel for the solid oxide fuel cell (SOFC) appears to be very attractive, as shown by cell tests with electrolyte supported cells (ESC) as well as anode supported cells (ASC) with an active area of 81cm2. The cell voltage was measured as function of the electrical current, temperature, gas composition and ammonia (NH3) flow. With NH3 as fuel, electrical cell efficiencies up to 70% (LHV) can be achieved at 0.35A∕cm2 and 60% (LHV) at 0.6A∕cm2. The cell degradation during 3000 h of operation was comparable with H2 fueled measurements. Due to the high temperature and the catalytic active Ni∕YSZ anode, NH3 cracks at the anode into H2 and N2 with a conversion of >99.996%. The high NH3 conversion is partly due to the withdrawal of H2 by the electrochemical cell reaction. The remaining NH3 will be converted in the afterburner of the system. The NOx outlet concentration of the fuel cell is low, typically <0.5ppm at temperatures below 950°C and around 4ppm at 1000°C. A SOFC system fueled with ammonia is relative simple compared with a carbon containing fuel, since no humidification of the fuel is necessary. Moreover, the endothermic ammonia cracking reaction consumes part of the heat produced by the fuel cell, by which less cathode cooling air is required compared with H2 fueled systems. Therefore, the system for a NH3 fueled SOFC will have relatively low parasitic power losses and relative small heat exchangers for preheating the cathode air flow.
ChemInform Abstract: SYNTHESIS OF AGESTRICIN A, B, C AND D (1984)

Abstract Aus dem Acetophenon (I) werden mit den entsprechenden Aldehyden die Chalkone (IIa)‐(IIc) hergestellt, die sauer zu den Flavanonen (IIIa)‐(IIIc) cyclisiert werden.
Binding Analyses of <i>Bacillus thuringiensis</i> Cry δ-Endotoxins Using Brush Border Membrane Vesicles of <i>Ostrinia nubilalis</i> (2001)

Transgenic corn expressing the Bacillus thuringiensis Cry1Ab gene is highly insecticidal to Ostrinia nubilalis (European corn borer) larvae. We ascertained whether Cry1F, Cry9C, or Cry9E recognizes the Cry1Ab binding site on the O. nubilalis brush border by three approaches. An optical biosensor technology based on surface plasmon resonance measured binding of brush border membrane vesicles (BBMV) injected over a surface of immobilized Cry toxin. Preincubation with Cry1Ab reduced BBMV binding to immobilized Cry1Ab, whereas preincubation with Cry1F, Cry9C, or Cry9E did not inhibit BBMV binding. BBMV binding to a Cry1F-coated surface was reduced when vesicles were preincubated in Cry1F or Cry1Ab but not Cry9C or Cry9E. A radioligand approach measured 125I-Cry1Ab toxin binding to BBMV in the presence of homologous (Cry1Ab) and heterologous (Cry1Ac, Cry1F, Cry9C, or Cry9E) toxins. Unlabeled Cry1Ac effectively competed for 125I-Cry1Ab binding in a manner comparable to Cry1Ab itself. Unlabeled Cry9C and Cry9E toxins did not inhibit (125)I-Cry1Ab binding to BBMV. Cry1F inhibited (125)I-Cry1Ab binding at concentrations greater than 500 nM. Cry1F had low-level affinity for the Cry1Ab binding site. Ligand blot analysis identified Cry1Ab, Cry1Ac, and Cry1F binding proteins in BBMV. The major Cry1Ab signals on ligand blots were at 145 kDa and 154 kDa, but a strong signal was present at 220 kDa and a weak signal was present at 167 kDa. Cry1Ac and Cry1F binding proteins were detected at 220 and 154 kDa. Anti-Manduca sexta aminopeptidase serum recognized proteins of 145, 154, and 167 kDa, and anti-cadherin serum recognized the 220 kDa protein. We speculate that isoforms of aminopeptidase and cadherin in the brush border membrane serve as Cry1Ab, Cry1Ac, and Cry1F binding proteins.
Electroconvulsive Therapy in a Patient with Mania, Parkinsonism, and Tardive Dyskinesia. (1988)

The authors describe a patient in whom ECT was associated with recovery from a manic episode, improvement in coexistent parkinsonism, and a marked change in the anatomic distribution of dyskinetic movements.
Supervised ranking in open-domain text summarization (2001)

The paper proposes and empirically motivates an integration of supervised learning with unsupervised learning to deal with human biases in summarization. In particular, we explore the use of probabilistic decision tree within the clustering framework to account for the variation as well as regularity in human created summaries. The corpus of human created extracts is created from a newspaper corpus and used as a test set. We build probabilistic decision trees of different flavors and integrate each of them with the clustering framework. Experiments with the corpus demonstrate that the mixture of the two paradigms generally gives a significant boost in performance compared to cases where either of the two is considered alone.
Trends bei der Verarbeitung von Ölsamen und ‐früchten. Die gleichzeitige Gewinnung von Ölen und Proteinen (1975)

Abstract Anhand einer Literaturstudie werden die bei der gleichzeitigen Gewinnung von Ölen und Proteinen im Laboratoriums‐ bzw. Pilotmaßstab erforderlichen Verfahrensschritte — Intensivzerkleinerung der Zellen, Abtrennung unlöslicher Zellbestandteile, Aufarbeitung der anfallenden Emulsion bzw. Ölabscheidung und Proteinisolierung — beschrieben. Weiterhin werden solche Verfahrensvarianten mit den traditionellen Prozessen der Öl‐ und Proteingewinnung aus Ölsamen und ‐früchten bzw. deren Verarbeitungsrückständen verglichen, wobei eine Wertung vorgenommen und auf die anstehenden Probleme hingewiesen wird.
Multimedia Presentation Authoring and Virtual Collaboration in Medicine (2010)

Web-based virtual collaboration is increasingly gaining popularity in almost every area in our society due to the fact that it can bridge the gap imposed by time and geographical constraints. However, in medical field, such collaboration has been less popular than other fields. Some of the reasons were timeliness, security, and preciseness of the information they are dealing with. In this paper, we are proposing a web-based distributed medical collaboration system called Virtual Collaboration System for Medicine (VCSM) for medical doctors that meet the needs. The proposed system consists of two parts - multimedia presentation and recordable virtual collaboration. The former supports synchronized multimedia presentation using Synchronous Multimedia Integration Language (SMIL.) It allows synchronization of the contents of a PowerPoint presentation file and a video file. The presentation may be provided to the participants before the discussion begins. Next, in the virtual collaboration stage, participants can use text along with associated symbols during the discussion over the presented medical images. The symbols such as arrows or polygons can be set or removed dynamically to represent areas of interest in digital images using so called layered architecture that separates image layer from annotation layer. XML files are used to record participants' opinions along with the symbols over some particular images
Energy absorbing system for mechanical impacts (1972)

System is described based on use of arrangement of crushable hollow spheres bonded together in layers of progressively different diameter, with largest diameter spheres positioned to receive impact forces initially. System is particularly useful for delivery of payloads by air-drop techniques.