gebhart/patrepeval huggingface dataset:
Efficient Unrestricted Identity-Based Aggregate Signature Scheme (2014)

An aggregate signature scheme allows anyone to compress multiple individual signatures from various users into a single compact signature. The main objective of such a scheme is to reduce the costs on storage, communication and computation. However, among existing aggregate signature schemes in the identity-based setting, some of them fail to achieve constant-length aggregate signature or require a large amount of pairing operations which grows linearly with the number of signers, while others have some limitations on the aggregated signatures. The main challenge in building efficient aggregate signature scheme is to compress signatures into a compact, constant-length signature without any restriction. To address the above drawbacks, by using the bilinear pairings, we propose an efficient unrestricted identity-based aggregate signature. Our scheme achieves both full aggregation and constant pairing computation. We prove that our scheme has existential unforgeability under the computational Diffie-Hellman assumption.
Platelet‐activating factor stimulates phosphatidic acid formation in cultured rat mesangial cells: Roles of phospholipase D, diglyceride kinase, and de novo phospholipid synthesis (1993)

Platelet-activating factor (PAF) stimulates phospholipase C (PLC)-induced hydrolysis of phosphatidylinositol-4,5-bisphosphate (PtdIns-4,5-P2). Yet, PAF-stimulated diglycerides (DG) are still elevated at time points where inositol polyphosphates have returned to basal levels. Thus, other signal transduction pathways that hydrolyze phosphatidylcholine (PtdCho) or phosphatidylethanolamine (PtdEth) and form DG and phosphatidic acids (PA) through either PLC or phospholipase D (PLD) may also mediate PAF-stimulated cellular responses. Initially the effects of PAF upon 32P-PA generation in mesangial cells (MC) were assessed. PA formation may be indicative of several metabolic pathways including PLD and DG kinase activities as well as de novo phospholipid synthesis. PAF (10(-7) M) increased 32P-PA formation as early as 5 seconds and this elevation persisted up to 15 minutes. When MC were pretreated with the DG kinase inhibitor-R59022, PAF-induced 32P-PA formation was diminished at early but not late time points, demonstrating that the initial component of PA formation may be due, in part, to PLC activation and subsequent phosphorylation of DG. The reciprocal reaction, PA phosphohydrolase, which dephosphorylates PA to from DG was not stimulated by PAF, suggesting that the sustained elevation of DG induced by PAF is primarily a reflection of PLC. 3H-glycerol pulse-labeling experiments suggest that PAF also stimulates de novo phospholipid synthesis which also contributes to PA formation. Conclusive proof for PLD in the generation of PA was obtained by assessing the formation of 3H-phosphatidyl-ethanol (PEt) from 3H-alkyl-lyso-glycero phosphocholine (GPC) and exogenous ethanol. PAF stimulated alkyl-PEt generation in the presence but not the absence of 0.5% ethanol. Also, PAF induced a concomitant elevation of alkyl-PA at 15 minutes and this elevation of alkyl-PA was reduced when the cells were exposed to exogenous ethanol, reflecting the formation of PEt. Corroborating evidence suggests that PAF stimulates 3H-choline and 3H-ethanolamine release, suggesting that PtdCho and PtdEth are substrates for PLD. Thus, these data demonstrate that MC respond to PAF with elevated PLD and DG kinase activities as well as with an increased rate of de novo lipid synthesis which increases PA, a potential intracellular signal.
The digitisation of healthcare in a developing country - the case of ICT institutionalisation in a private hospital (2018)

Calls have been made to look at the implementation of ICT at the small-scale level which involves technologies with low cost of adoption or in resource-poor regions. Hence, this study investigates the opportunities and the challenges involved in the implementation of ICT in a private hospital in a developing economy. A descriptive case study strategy was therefore adopted where data was collected through interviews, artefact and document observation. It was discovered that a Hospital Management Administration System (HAMS) has been introduced. In addition, the study identified some challenges in implementation. Lessons and recommendations for overcoming the challenges in the implementation of ICT in the hospital are discussed. The findings add to the existing knowledge bank of ICT adoption and implementation in the health sector specifically by a private hospital in a developing economy. This research is beneficial to government agencies responsible for the health sector.
Interleaved Converter With Voltage Multiplier Cell for High Step-Up and High-Efficiency Conversion (2010)

A novel interleaved high step-up converter with voltage multiplier cell is proposed in this paper to avoid the extremely narrow turn-off period and to reduce the current ripple, which flows through the power devices compared with the conventional interleaved boost converter in high step-up applications. Interleaved structure is employed in the input side to distribute the input current, and the voltage multiplier cell is adopted in the output side to achieve a high step-up gain. The voltage multiplier cell is composed of the secondary windings of the coupled inductors, a series capacitor, and two diodes. Furthermore, the switch voltage stress is reduced due to the transformer function of the coupled inductors, which makes low-voltage-rated MOSFETs available to reduce the conduction losses. Moreover, zero-current-switching turn- on soft-switching performance is realized to reduce the switching losses. In addition, the output diode turn-off current falling rate is controlled by the leakage inductance of the coupled inductors, which alleviates the diode reverse recovery problem. Additional active device is not required in the proposed converter, which makes the presented circuit easy to design and control. Finally, a 1-kW 40-V-input 380-V-output prototype operating at 100 kHz switching frequency is built and tested to verify the effectiveness of the presented converter.
Perhaloalkyl hypochlorites and pentafluorosulfur hypochlorite. IV. Reactions with olefins (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPerhaloalkyl hypochlorites and pentafluorosulfur hypochlorite. IV. Reactions with olefinsLowell Ray Anderson, David Edward Young, Douglas E. Gould, R. Juurik-Hogan, D. Neuchterlein, and William B. FoxCite this: J. Org. Chem. 1970, 35, 11, 3730–3733Publication Date (Print):November 1, 1970Publication History Published online1 May 2002Published inissue 1 November 1970https://pubs.acs.org/doi/10.1021/jo00836a032https://doi.org/10.1021/jo00836a032research-articleACS PublicationsRequest reuse permissionsArticle Views164Altmetric-Citations22LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
In Vitro Synthesis of Betaxanthins Using Recombinant DOPA 4,5-Dioxygenase and Evaluation of Their Radical-Scavenging Activities (2010)

Betalamic acid, the chromophore of betaxanthins, was enzymatically synthesized on a large scale from l-dihydroxyphenylalanine (L-DOPA) using recombinant Mirabilis jalapa DOPA 4,5-dioxygenase. After synthesis, proline was directly added to the concentrated reaction mixture to generate proline-betaxanthin. The molecular mass and nuclear magnetic resonance spectrum of the purified product were identical to those previously reported for proline-betaxanthin. Twenty-four betaxanthin species were synthesized by the condensation reaction of purified betalamic acid and amino acids or amines. An HPLC protocol was established for identifying the different betaxanthin species. Proline-, dopamine-, and γ-aminobutyric acid (GABA)-betaxanthins were prepared as representative betaxanthins under large-scale conditions, and their 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activities were compared against those of known antioxidants. GABA-betaxanthin showed comparatively low activity, whereas dopamine-betaxanthin had similar activity to the red pigment betanin and the anthocyanin cyanidin 3-glucoside. Proline-betaxanthin had the highest activity of the three synthesized compounds and was similar to the flavonoid quercetin.
Insulin-like Growth Factor I (IGF-I) Induces Unique Effects in the Cytoskeleton of Cultured Rat Glomerular Mesangial Cells (1997)

Resident glomerular mesangial cells (MCs) have complex cytoskeletal organizations that maintain functional and structural integrity. The ability of cells to replicate, coordinate movement, change shape, and interact with contiguous cells or extracellular matrix depends on cytoskeletal organization. MCs synthesize insulin-like growth factor (IGF-I), express IGF-I receptors, and respond to IGF-I with increased proliferation. We noted that IGF-I treatment of mesangial cells was associated with a change in morphology. Therefore, these studies were undertaken to define specific IGF-I-mediated changes in cytoskeletal protein organization. Rat MCs were propagated from birth in culture without supplemental insulin. Quiescent, subconfluent cultures were treated with IGF-I (100 nM) for 1 hr. Rearrangements in f-actin, alpha-smooth muscle actin, beta-actin, vimentin, and vinculin were seen by fluorescence microscopy. As the cytoskeleton rearranged, alpha-smooth muscle actin dissociated from the f-actin bundles and beta-actin became polymerized under the leading lamellar edge. Ultrastructural changes were consistent with increased membrane turnover and metabolic activity. The normally sessile mesangial cell was induced by IGF-I to express a wound-healing phenotype characterized by movement and increased pinocytosis. These changes are different from those induced by insulin and have important implications for mesangial cell function.
Photodynamic Therapy of Intracranial Tissues: A Preclinical Comparative Study of Four Different Photosensitizers (1998)

For clinical PDT of most adult intracranial neoplasms ALA-induced PpIX appears to be promising, and SnET2 (liposomal) has potential for selective tumor destruction with relative sparing of white matter. Other normal brain structures and, for the other photosensitizers, also white matter are at risk of collateral damage, if exposed to light during PDT.
A Temporal Mode Selection In The Mpeg-2 Encoder Scheme (1996)

Publication in the conference proceedings of EUSIPCO, Trieste, Italy, 1996
Overcoming the “Oxidant Problem”: Strategies to Use O<sub>2</sub> as the Oxidant in Organometallic C–H Oxidation Reactions Catalyzed by Pd (and Cu) (2012)

Oxidation reactions are key transformations in organic chemistry because they can increase chemical complexity and incorporate heteroatom substituents into carbon-based molecules. This principle is manifested in the conversion of petrochemical feedstocks into commodity chemicals and in the synthesis of fine chemicals, pharmaceuticals, and other complex organic molecules. The utility and function of these molecules correlate directly with the presence and specific placement of oxygen and nitrogen heteroatoms and other functional groups within the molecules. Methods for selective oxidation of C-H bonds have expanded significantly over the past decade, and their role in the synthesis of organic chemicals will continue to increase. Our group's contributions to this field are linked to our broader interest in the development and mechanistic understanding of aerobic oxidation reactions. Molecular oxygen (O(2)) is the ideal oxidant. Its low cost and lack of toxic byproducts make it a highly appealing reagent that can address key "green chemistry" priorities in industry. With strong economic and environmental incentives to use O(2), the commmodity chemicals industry often uses aerobic oxidation reactions. In contrast, O(2) is seldom used to prepare more-complex smaller-volume chemicals, a limitation that reflects, in part, the limited synthetic scope and utility of existing aerobic reactions. Pd-catalyzed reactions represent some of the most versatile methods for selective C-H oxidation, but they often require stoichiometric transition-metal or organic oxidants, such as Cu(II), Ag(I), or benzoquinone. This Account describes recent strategies that we have identified to use O(2) as the oxidant in these reactions. In Pd-catalyzed C-H oxidation reactions that form carbon-heteroatom bonds, the stoichiometric oxidant is often needed to promote difficult reductive elimination steps in the catalytic mechanism. To address this challenge, we have identified new ancillary ligands for Pd that promote reductive elimination, or replaced Pd with a Cu catalyst that undergoes facile reductive elimination from a Cu(III) intermediate. Both strategies have enabled O(2) to be used as the sole stoichiometric oxidant in the catalytic reactions. C-H oxidation reactions that form the product via β-hydride or C-C reductive elimination steps tend to be more amenable to the use of O(2). The use of new ancillary ligands has also overcome some of the limitations in these methods. Mechanistic studies are providing insights into some (but not yet all) of these advances in catalytic reactivity.
Crystal Structure of a Human Cyclin-Dependent Kinase 6 Complex with a Flavonol Inhibitor, Fisetin (2005)

Cyclin-dependent kinases (CDKs) play a central role in cell cycle control, apoptosis, transcription, and neuronal functions. They are important targets for the design of drugs with antimitotic or antineurodegenerative effects. CDK4 and CDK6 form a subfamily among the CDKs in mammalian cells, as defined by sequence similarities. Compared to CDK2 and CDK5, structural information on CDK4 and CDK6 is sparse. We describe here the crystal structure of human CDK6 in complex with a viral cyclin and a flavonol inhibitor, fisetin. Fisetin binds to the active form of CDK6, forming hydrogen bonds with the side chains of residues in the binding pocket that undergo large conformational changes during CDK activation by cyclin binding. The 4-keto group and the 3-hydroxyl group of fisetin are hydrogen bonded with the backbone in the hinge region between the N-terminal and C-terminal kinase domain, as has been observed for many CDK inhibitors. However, CDK2 and HCK kinase in complex with other flavone inhibitors such as quercetin and flavopiridol showed a different binding mode with the inhibitor rotated by about 180 degrees. The structural information of the CDK6-fisetin complex is correlated with the binding affinities of different flavone inhibitors for CDK6. This complex structure is the first description of an inhibitor complex with a kinase from the CDK4/6 subfamily and can provide a basis for selecting and designing inhibitor compounds with higher affinities and specificities.
CATS: a Cryogenic Automated Transfer System installed on the beamline FIP at ESRF (2004)

CATS allows users to mount and dismount their crystal samples remotely on the diffractometer, without entering the experimental hutch. CATS has been integrated into the automated control of FIP, allowing users to choose the wavelengths, optimize the beam intensity, mount and screen their crystal sample automatically and finally record diffraction data on the best sample(s).
A new approach to integrate seismic and production data in reservoir models (1997)

A great deal of effort is devoted to reducing the uncertainties in reservoir modeling. For example, seismic properties are used to improve the characterization of interwell properties by providing porosity maps constrained to seismic impedance. Another means to reduce uncertainties is to constrain the reservoir model to production data. This paper describes a new approach where the production and seismic data are simultaneously used to reduce the uncertainties. In this new approach, the primary geologic parameter that controls reservoir properties is identified. Next, the geophysical parameter that is sensitive to the dominant geologic parameter is determined. Then the geology and geophysics are linked using analytic correlations. Unfortunately, the initial guess resulted in a reservoir model that did not match the production history. Since the time required for trial and error matching of production history is exorbitant, an automatic history matching method based on a fast optimization method was used to find the correlating parameters. This new approach was illustrated with an actual field in the Williston Basin. Upscalling problems do not arise since the scale is imposed by the size of the seismic bin (66m, 219 ft) which is the size of the simulator gridblocks.
Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium <i>Alicyclobacillus acidocaldarius</i> Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH (2000)

We have studied the uptake of maltose in the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius, which grows best at 57 degrees C and pH 3.5. Under these conditions, accumulation of [(14)C]maltose was observed in cells grown with maltose but not in those grown with glucose. At lower temperatures or higher pH values, the transport rates substantially decreased. Uptake of radiolabeled maltose was inhibited by maltotetraose, acarbose, and cyclodextrins but not by lactose, sucrose, or trehalose. The kinetic parameters (K(m) of 0.91 +/- 0.06 microM and V(max) ranging from 0.6 to 3.7 nmol/min/mg of protein) are consistent with a binding protein-dependent ATP binding cassette (ABC) transporter. A corresponding binding protein (MalE) that interacts with maltose with high affinity (K(d) of 1.5 microM) was purified from the culture supernatant of maltose-grown cells. Immunoelectron microscopy revealed distribution of the protein throughout the cell wall. The malE gene was cloned and sequenced. Five additional open reading frames, encoding components of a maltose transport system (MalF and MalG), a putative transcriptional regulator (MalR), a cyclodextrinase (CdaA), and an alpha-glucosidase (GlcA), were identified downstream of malE. The malE gene lacking the DNA sequence that encodes the signal sequence was expressed in Escherichia coli. The purified wild-type and recombinant proteins bind maltose with high affinity over a wide pH range (2.5 to 7) and up to 80 degrees C. Recombinant MalE cross-reacted with an antiserum raised against the wild-type protein, thereby indicating that the latter is the product of the malE gene. The MalE protein might be well suited as a model to study tolerance of proteins to low pH.
Association of Inpatient Use of Angiotensin-Converting Enzyme Inhibitors and Angiotensin II Receptor Blockers With Mortality Among Patients With Hypertension Hospitalized With COVID-19 (2020)

Among hospitalized patients with COVID-19 and coexisting hypertension, inpatient use of ACEI/ARB was associated with lower risk of all-cause mortality compared with ACEI/ARB nonusers. While study interpretation needs to consider the potential for residual confounders, it is unlikely that in-hospital use of ACEI/ARB was associated with an increased mortality risk.
Optical sensor for continuous determination of halides (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOptical sensor for continuous determination of halidesEdmund. Urbano, Helmut. Offenbacher, and Otto S. WolfbeisCite this: Anal. Chem. 1984, 56, 3, 427–429Publication Date (Print):March 1, 1984Publication History Published online1 May 2002Published inissue 1 March 1984https://pubs.acs.org/doi/10.1021/ac00267a029https://doi.org/10.1021/ac00267a029research-articleACS PublicationsRequest reuse permissionsArticle Views500Altmetric-Citations96LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
An MHC class Ib–restricted CD8 T cell response confers antiviral immunity (2008)

Although immunity against intracellular pathogens is primarily provided by CD8 T lymphocytes that recognize pathogen-derived peptides presented by major histocompatibility complex (MHC) class Ia molecules, MHC class Ib-restricted CD8 T cells have been implicated in antiviral immunity. Using mouse polyoma virus (PyV), we found that MHC class Ia-deficient (K(b-/-)D(b-/-)) mice efficiently control this persistently infecting mouse pathogen. CD8 T cell depletion mitigates clearance of PyV in K(b-/-)D(b-/-) mice. We identified the ligand for PyV-specific CD8 T cells in K(b-/-)D(b-/-) mice as a nonamer peptide from the VP2 capsid protein presented by Q9, a member of the beta(2) microglobulin-associated Qa-2 family. Using Q9-VP2 tetramers, we monitored delayed but progressive expansion of these antigen-specific CD8alphabeta T cells in K(b-/-)D(b-/-) mice. Importantly, we demonstrate that Q9-VP2-specific CD8 T cells more effectively clear wild-type PyV than a VP2 epitope(null) mutant PyV. Finally, we show that wild-type mice also generate Q9-restricted VP2 epitope-specific CD8 T cells to PyV infection. To our knowledge, this is the first evidence for a defined MHC class Ib-restricted antiviral CD8 T cell response that contributes to host defense. This study motivates efforts to uncover MHC class Ib-restricted CD8 T cell responses in other viral infections, and given the limited polymorphism of MHC class Ib molecules, it raises the possibility of developing peptide-based viral vaccines having broad coverage across MHC haplotypes.