gebhart/patrepeval huggingface dataset:
Redox regulation of cell proliferation: Bioinformatics and redox proteomics approaches to identify redox-sensitive cell cycle regulators (2018)

Plant stem cells are the foundation of plant growth and development. The balance of quiescence and division is highly regulated, while ensuring that proliferating cells are protected from the adverse effects of environment fluctuations that may damage the genome. Redox regulation is important in both the activation of proliferation and arrest of the cell cycle upon perception of environmental stress. Within this context, reactive oxygen species serve as 'pro-life' signals with positive roles in the regulation of the cell cycle and survival. However, very little is known about the metabolic mechanisms and redox-sensitive proteins that influence cell cycle progression. We have identified cysteine residues on known cell cycle regulators in Arabidopsis that are potentially accessible, and could play a role in redox regulation, based on secondary structure and solvent accessibility likelihoods for each protein. We propose that redox regulation may function alongside other known posttranslational modifications to control the functions of core cell cycle regulators such as the retinoblastoma protein. Since our current understanding of how redox regulation is involved in cell cycle control is hindered by a lack of knowledge regarding both which residues are important and how modification of those residues alters protein function, we discuss how critical redox modifications can be mapped at the molecular level.
Study on Edge Detection Based on Local Energy Model (2011)

Most of the edge detection algorithms are based on the gradient method.When texture is complex,the gradient may be cluttered.And the useful information cannot be found in the edge map.In this paper,a local energy model to detect robust and useful edges is given.The local energy model is related to the Human Vision System(HVS).And non-classic receptive field(Non-CRF) inhibition methods are used to detect interested edges.The results show the proposed method is much better than gradient methods in detecting edges of interesting.
Enzymatic mutation detection: enrichment of heteroduplexes from hybrid DNA mixtures by cleavage-deficient GST-tagged endonuclease VII (1999)

A method for the enrichment of heteroduplex DNAs from hybrid DNA mixtures by endonuclease VII is reported. The procedure is based on the ability of a GST-fused cleavage-deficient mutant endonuclease VII (EVII-N62D(GST)) to bind to mismatching nucleotides in heteroduplex DNAs identical to the wild-type enzyme. The GST tag was used for stable immobilisation of the protein to Glutathione Sepharose 4B. This enables the material to withstand the repeated rounds of binding steps required for enrichment of heteroduplex molecules from appropriate samples.
Simultaneous all-optical frequency downconversion technique utilizing an SOA-MZI for WDM radio over fiber (RoF) applications (2006)

Simultaneous all-optical frequency-downconversion technique utilizing a semiconductor optical amplifier Mach-Zehnder interferometer (SOA-MZI) is experimentally demonstrated, and its application to a wavelength-division-multiplexing (WDM) radio over fiber (RoF) uplink is proposed. The conversion efficiencies from 22.5 (f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">RF</sub> ) to 2.5 GHz (f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">IF</sub> =f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">RF</sub> -2f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">LO</sub> ) are in the range from 1.5 to 3 dB for the optical RF wavelength between 1548 and 1558 nm. Error-free simultaneous all-optical frequency downconversion of the two WDM RoF upstream channels that carry 155-Mb/s differential phase-shift keying data at 22.5 GHz to an optical intermediate frequency signal having the frequency of 2.5 GHz with the power penalty less than 0.1 dB at the bit error rate of 10 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-8</sup> is achieved
Sequential Operations in Digital Picture Processing (1966)

article Free Access Share on Sequential Operations in Digital Picture Processing Authors: Azriel Rosenfeld Computer Science Center, University of Maryland, College Park, Maryland Computer Science Center, University of Maryland, College Park, MarylandView Profile , John L. Pfaltz Computer Science Center, University of Maryland, College Park, Maryland Computer Science Center, University of Maryland, College Park, MarylandView Profile Authors Info & Claims Journal of the ACMVolume 13Issue 4Oct. 1966 pp 471–494https://doi.org/10.1145/321356.321357Published:01 October 1966Publication History 1,217citation4,367DownloadsMetricsTotal Citations1,217Total Downloads4,367Last 12 Months448Last 6 weeks41 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Using the Media (1997)

The average British voter is not an enthusiastic consumer of party political propaganda. Few people set their video so as not to miss a party political broadcast, scour the streets for party billboards, or rush to their letterbox when they hear it open and shut in the hope of finding the latest circular from a local candidate. And the rare individual who is an avid peruser of a party's publicity material is probably a committed supporter and almost certainly not an all-important floating voter.KeywordsParty LeaderTelevision NewsPress ConferenceLabour PartyConservative PartyThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Analysis of C-cadherin Regulation during Tissue Morphogenesis with an Activating Antibody (1999)

The regulation of cadherin-mediated adhesion at the cell surface underlies several morphogenetic processes. To investigate the role of cadherin regulation in morphogenesis and to begin to analyze the molecular mechanisms of cadherin regulation, we have screened for monoclonal antibodies (mAbs) that allow us to manipulate the adhesive state of the cadherin molecule. Xenopus C-cadherin is regulated during convergent extension movements of gastrulation. Treatment of animal pole tissue explants (animal caps) with the mesoderm-inducing factor activin induces tissue elongation and decreases the strength of C-cadherin-mediated adhesion between blastomeres (Brieher, W.M., and B.M. Gumbiner. 1994. J. Cell Biol. 126:519-527). We have generated a mAb to C-cadherin, AA5, that restores strong adhesion to activin-treated blastomeres. This C-cadherin activating antibody strongly inhibits the elongation of animal caps in response to activin without affecting mesodermal gene expression. Thus, the activin-induced decrease in C-cadherin adhesive activity appears to be required for animal cap elongation. Regulation of C-cadherin and its activation by mAb AA5 involve changes in the state of C-cadherin that encompass more than changes in its homophilic binding site. Although mAb AA5 elicited a small enhancement in the functional activity of the soluble C-cadherin ectodomain (CEC1-5), it was not able to restore cell adhesion activity to mutant C-cadherin lacking its cytoplasmic tail. Furthermore, activin treatment regulates the adhesion of Xenopus blastomeres to surfaces coated with two other anti-C-cadherin mAbs, even though these antibodies probably do not mediate adhesion through a normal homophilic binding mechanism. Moreover, mAb AA5 restores strong adhesion to these antibodies. mAb AA5 only activates adhesion of blastomeres to immobilized CEC1-5 when it binds to C-cadherin on the cell surface. It does not work when added to CEC1-5 on the substrate. Together these findings suggest that the regulation of C-cadherin by activin and its activation by mAb AA5 involve changes in its cellular organization or interactions with other cell components that are not intrinsic to the isolated protein.
A Deep Memory-based Architecture for Sequence-to-Sequence Learning (2015)

We propose DEEPMEMORY, a novel deep architecture for sequence-to-sequence learning, which performs the task through a series of nonlinear transformations from the representation of the input sequence (e.g., a Chinese sentence) to the final output sequence (e.g., translation to English). Inspired by the recently proposed Neural Turing Machine (Graves et al., 2014), we store the intermediate representations in stacked layers of memories, and use read-write operations on the memories to realize the nonlinear transformations between the representations. The types of transformations are designed in advance but the parameters are learned from data. Through layer-by-layer transformations, DEEPMEMORY can model complicated relations between sequences necessary for applications such as machine translation between distant languages. The architecture can be trained with normal back-propagation on sequenceto-sequence data, and the learning can be easily scaled up to a large corpus. DEEPMEMORY is broad enough to subsume the state-of-the-art neural translation model in (Bahdanau et al., 2015) as its special case, while significantly improving upon the model with its deeper architecture. Remarkably, DEEPMEMORY, being purely neural network-based, can achieve performance comparable to the traditional phrase-based machine translation system Moses with a small vocabulary and a modest parameter size.
Mycobactin analysis as an aid for the identification of Mycobacterium fortuitum and Mycobacterium chelonae subspecies (1992)

Mycobactin patterns from 65 Mycobacterium fortuitum and Mycobacterium chelonae strains have been determined by thin-layer chromatography. By use of a rich liquid medium containing an iron chelator (ethylenediamine-di-o-hydroxyphenylacetic acid [EDDA]) to ensure iron starvation, all strains were able to form mycobactins. The method developed here allows sensitive detection of mycobactin by thin-layer chromatography from as little as 5 ml of culture after a 2-week incubation. Within M. fortuitum two mycobactin patterns were identified, whereas within M. chelonae four were recognized. Comparisons with the subspecific identification performed by using biochemical tests showed that 73% of the M. fortuitum subsp. fortuitum strains shared the same mycobactin pattern (designated F), whereas 75% of the M. fortuitum subsp. peregrinum strains shared the other mycobactin pattern (designated P). Within the M. fortuitum strains that cannot be assigned to a subspecies on the basis of their biochemical features, only F and P patterns were determined. Similarly, 93% of the M. chelonae subsp. chelonae strains produced the so-called C1 and C2 patterns and 86% of the M. chelonae subsp. abscessus strains produced A1 and A2 patterns. C2 and A2 were the patterns most frequently encountered; they were represented by 65 and 50% of the M. chelonae subsp. chelonae and M. chelonae subsp. abscessus strains, respectively. Within the biochemically M. chelonae strains that did not fit any subspecies on the basis of biochemical test results, C1, C2, and A1 patterns were found. Whereas about 30% of both M. fortuitum and M. chelonae strains cannot be characterized to the subspecies level on the basis of biochemical tests, 100% of the strains of both species can be characterized on the basis of mycobactin patterns.
Optimum Small-Scale Open and Direct Solar Thermal Brayton Cycle for Pretoria, South Africa (2012)

The open and direct solar thermal Brayton cycle is exposed to various weather conditions like changing solar irradiation, wind and surrounding temperature. The geometries of the receiver and recuperator and the turbine operating point as parameter can be optimised in such a way that they accommodate these weather changes and allow for high net power output throughout a typical year. In this paper, a method of obtaining these parameters based on total entropy generation minimisation is presented. A parabolic dish concentrator with a diameter of 4.8 m is used as well as an off-the-shelf turbo-machine. Results show that the net absorbed power at the receiver and maximum allowed receiver surface temperature play important roles in determining the optimum operating point and maximum net power output.
Mimicking biopolymers on a molecular scale: nano(bio)technology based on engineered proteins (2009)

Proteins are ubiquitous biopolymers that adopt distinct three-dimensional structures and fulfil a multitude of elementary functions in organisms. Recent systematic studies in molecular biology and biotechnology have improved the understanding of basic functional and architectural principles of proteins, making them attractive candidates as concept generators for technological development in material science, particularly in biomedicine and nano(bio)technology. This paper highlights the potential of molecular biomimetics in mimicking high-performance proteins and provides concepts for applications in four case studies, i.e. spider silk, antifreeze proteins, blue mussel adhesive proteins and viral ion channels.
The problem with EHRs and coding (2009)

Something has gone awry to create an environment that leaves well-intended physicians victimized when government audits reveal their software systems have allowed—even facilitated—submission of non-compliant and potentially fraudulent claims.
Sustained-release Bupropion Versus Naltrexone in the Treatment of Pathological Gambling (2005)

This preliminary study shows that sustained-release bupropion may be effective as naltrexone in the treatment of PG. Further studies are needed to confirm our findings.
Robustness studies in adaptive control (1983)

This paper examines robustness issues in Model Reference Adaptive Control systems in the presence of unmodeled dynamics and output disturbances. We present an approximate technique, trend analysis, by which we can study the evolution of the parameter error trajectory under periodic excitation. This analysis provides new insights upon the size and spectral content of the excitation sufficient to guarantee local stability.
The quest for sustainable polyesters – insights into the future (2013)

Polyesters from renewable resources are an expanding area with a burgeoning scientific activity, nevertheless little has been reviewed about this particular class of polymers. The present appraisal intends to contribute to fill this literature gap by reviewing recent aspects related to the most promising renewable-based polyesters. Emphasis will be placed on bio-based polyesters that, given their comparable properties, may replace polymers derived from fossil fuel feedstock, and on bio-based polyesters with completely innovative properties for novel applications. Furthermore, the sources of renewable monomers will also be reviewed, together with the most relevant eco-friendly synthetic approaches used in polycondensation reactions leading to polyesters.
Sensitive assay for thyroglobulin autoantibodies in serum employing polyethylene glycol for precipitation (1988)

A sensitive assay for thyroglobulin autoantibodies (Tg-ab) is described. The assay is based on prolonged incubation of [125I]Tg with serum followed by precipitation of antibody bound [125I]Tg by polyethylene glycol (PEG). The PEG concentration was chosen to give a low precipitation of free [125I]Tg while effectively precipitating Tg-ab-bound [125I]Tg. The lowest reference employed contained 19.5 MRC U/l Tg-ab. This was always different from an incubate without Tg-ab. Tg standard added to samples interfered in the assay giving spuriously low Tg-ab values as in other Tg-ab assays. Tg-ab added to samples was recovered quantitatively. The interassay coefficient of variation was 19.8% and the intra-assay coefficient of variation 11.3%. Only eight of 66 serum samples with various amounts of Tg-ab, measured with the new assay, had levels of Tg-ab detectable by the passive haemagglutination test.
Tissue Distribution and Membrane Localization of Aquaporin-9 Water Channel (2001)

Aquaporin-9 (AQP9) is a water channel membrane protein also permeable to small solutes such as urea, glycerol, and 5-fluorouracil, a chemotherapeutic agent. With the aim of understanding the pathophysiological role of AQP9, we performed an extensive analysis by Western blotting, RT-PCR, and immunolocalization in rat tissues. Western blotting analysis revealed a major band of approximately 32 kD in testis, liver, and brain. Immunofluorescence showed strong expression of AQP9 in the plasma membrane of testis Leydig cells. In liver, AQP9 expression was found to be sex-linked. Male rats had higher levels of AQP9 than female in terms of both protein and mRNA. Moreover, in female livers the expression of AQP9 was mostly confined to perivascular hepatocytes, whereas males showed a more homogeneous hepatocyte staining. No differences in AQP9 expression level related to the age or to protein content of the diet were found, indicating that differences in the liver may be gender-dependent. In the brain, AQP9 expression was found in tanycytes mainly localized in the areas lacking a blood-brain barrier (BBB), such as the circumventricular organs (CVOs) of the third ventricles, the subfornical organ, the hypothalamic regions, and the glial processes of the pineal gland. AQP9 expression in the osmosensitive region of the brain suggests a role in the mechanism of central osmoreception. All these findings show a unique tissue distribution of AQP9 compared to the other known aquaporins.
A general synthetic route to nanofibers of polyaniline derivatives (2006)

Nanofibrous mats of a wide variety of polyaniline derivatives can be synthesized without the need for templates or functional dopants by simply introducing an initiator into the reaction mixture of a rapidly mixed reaction between monomer and oxidant.
Zinc Thiolate Complexes [ZnL<i><sub>n</sub></i>(SR)]<sup>+</sup> with Azamacrocyclic Ligands: Synthesis and Structural Properties (2006)

Abstract A series of 24 novel zinc thiolate complexes containing saturated macrocyclic polyamine ligands with the general formula [Zn(L n )(SR)]ClO 4 (L n : n ‐dentate azamacrocyclic ligand) has been synthesized. Two different thiolate ligands (R = phenyl, benzyl) and 12 macrocyclic ligands with ring sizes varying from 11 to 16 atoms and containing three, four, and five nitrogen donors (including a new macrocyclic triamine ligand, 8‐methyl‐[11]aneN 3 ) have been used. The solid‐state structures of 20 of the 24 complexes have been characterized by X‐ray diffraction. The crystal lattice of all compounds is comprised exclusively of monomers. The tetracoordinate N 3 S compounds exhibit Zn–S bond lengths ranging from 2.2259(5) to 2.2492(9) Å and the pentacoordinate N 4 S systems from 2.266(2) to 2.3200(7) Å. In addition, two complexes with the very rare N 5 S coordination environment for zinc have been found. They exhibit Zn–S bond lengths of 2.3782(7) and 2.42 Å (mean value of two independent molecules). The thiolate complexes are suitable model systems for the precursor proteins of matrix metalloproteases (pro‐MMPs) as well as for thiolate alkylating enzymes such as cobalamine‐dependent/independent methionine synthases, farnesyl transferase, and the Ada repair protein. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006)
Water Proton NMR: A Tool for Protein Aggregation Characterization (2017)

Formulation stability is a critical attribute of any protein-based biopharmaceutical drug due to a protein's inherent tendency to aggregate. Advanced analytical techniques currently used for characterization of protein aggregates are prone to a number of limitations and usually require additional manipulations with the sample, such as dilution, separation, labeling, and use of special cuvettes. In the present work, we compared conventional techniques for the analysis of protein aggregates with a novel approach that employs the water proton transverse relaxation rate R<sub>2</sub>(<sup>1</sup>H<sub>2</sub>O). We explored differences in the sensitivity of conventional techniques, size-exclusion chromatography (SEC), microflow imaging (MFI), and dynamic light scattering (DLS), and water NMR (wNMR) toward the presence of monoclonal antibody aggregates generated by different stresses. We demonstrate that wNMR outperformed SEC, DLS, and MFI in that it was most consistently sensitive to increases in both soluble and insoluble aggregates, including subvisible particles. The simplicity of wNMR, its sensitivity, and possibility of noninvasive measurements are unique advantages that would permit its application for more efficient and higher throughput optimization of protein formulations.
Textual inference by combining multiple Logic programming paradigms (2005)

The goal of our approach to textual inference is to answer queries about events and dates reported in texts; queries that demand inferencing with the relevant background knowledge. Our primary knowledge representation language is AnsProlog. The core inference engine is a combination of AnsProlog and Constraint Logic programming. AnsProlog is particularly useful for the representation of defaults, causal relations, and other types of common-sense knowledge. Constraint Logic Programming is needed to solve constraints involving the relationship between the sequence of actions and the dates on which the actions may have occurred. The combination of the two creates a useful tool for answering queries over texts and in particular queries that involve relatively deep reasoning.
Management, Orchestration and Chargingin the New Era (2018)

In December 2017, 3GPP passed two major milestones for 5G by approving the first set of 5G New Radio (NR) specifications and by putting in place the 5G Phase 1 System Architecture. These achievements have brought about the need for new management standards, as 5G adds to the ever-growing size and complexity of telecom systems.
Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications (2014)

Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for epidemics of debilitating arthritic disease. The recent outbreak (2004-2014) resulted in an estimated 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. The development of CHIKV-specific diagnostics and research tools is thus highly desirable. Herein we describe the generation and characterization of the first mAbs specific for the capsid protein (CP) of CHIKV. The antibodies recognized isolates representing the major genotypes of CHIKV, as well as several other alphaviruses, and were reactive in a range of assays including ELISA, Western blot, immunofluorescence and immunohistochemistry (IHC). We have also used the anti-CP mAb 5.5G9 in IHC studies to show that capsid antigen is persistently expressed 30 days post-infection in cells with macrophage morphology in a mouse model of chronic CHIKV disease. These antibodies may thus represent useful tools for further research, including investigations into the structure and function of CHIKV CP, and as valuable reagents for CHIKV detection in a range of settings.
Hong Kong: The Business Environment in the New Special Administrative Region (2000)

There are both opportunities and dangers facing business in Hong Kong as a function of the changeover of sovereignty which took place on 1 July 1997. On that date, over 150 years of British administration (with the exception of the Japanese occupation from 1941 to 1945) came to an end and the People’s Republic of China (PRC) assumed power over the territory. The question that must be asked is whether or not the dynamic economic growth and favourable business environment of Hong Kong can be sustained under new management.