gebhart/patrepeval huggingface dataset:
WNT-3A modulates articular chondrocyte phenotype by activating both canonical and noncanonical pathways (2011)

Activation and disruption of Wnt/β-catenin signaling both result in cartilage breakdown via unknown mechanisms. Here we show that both WNT-3A and the Wnt inhibitor DKK1 induced de-differentiation of human articular chondrocytes through simultaneous activation of β-catenin–dependent and independent responses. WNT-3A activates both the β-catenin–dependent canonical pathway and the Ca2+/CaMKII noncanonical pathways, with distinct transcriptional targets. WNT-3A promotes cell proliferation and loss of expression of the chondrocyte markers COL2A1, Aggrecan, and SOX9; however, proliferation and AXIN2 up-regulation are downstream of the canonical pathway and are rescued by DKK1, whereas the loss of differentiation markers is CaMKII dependent. Finally, we showed that in chondrocytes, the Ca2+/CaMKII-dependent and β-catenin–dependent pathways are reciprocally inhibitory, thereby explaining why DKK1 can induce loss of differentiation through de-repression of the CaMKII pathway. We propose a novel model in which a single WNT can simultaneously activate different pathways with distinct and independent outcomes and with reciprocal regulation. This offers an opportunity for selective pharmacological targeting.
Odor considerations in the use of frying oils (1979)

Abstract Odors generated into cooking and serving areas during use of oils in pan frying and deep‐fat frying are of concern to home and institutional consumers and, in some respects, to industrial users. Odor considerations are factors in the selection of types of oils to be used in both domestic and foreign markets. Comparative techniques have been developed to evaluate room odor characteristics of frying oils. Evaluation research has been done on various oils and cooking fats for room odors developed during frying. Improved odor characteristics contributed by additives to oils have been studied, as well as relationship between the linolenate content of soybean oil and its characteristic room odor. The nature of the volatile constituents which contribute to room odors during frying is the subject of continuing research efforts.
Plasmid DNA Vaccines: Investigation of Integration into Host Cellular DNA following Intramuscular Injection in Mice (2000)

The primary safety concern for DNA vaccines is their potential to integrate into the host cell genome. We describe an integration assay based on purification of high-molecular-weight genomic DNA away from free plasmid using gel electrophoresis, such that the genomic DNA can then be assayed for integrated plasmid using a sensitive PCR method. The assay sensitivity was approximately 1 plasmid copy/microg DNA (representing approximately 150,000 diploid cells). Using this assay, we carried out integration studies of three different plasmid DNA vaccines, containing either the influenza hemagglutinin, influenza matrix or HIV gag gene. Six weeks after intramuscular injection, free plasmid was detected in treated muscle at levels ranging from approximately 1,000 to 4,000 copies/microg DNA. At 6 months, the plasmid levels ranged between 200 and 800 copies/microg DNA. Gel purification of genomic DNA revealed that essentially all of the detectable plasmid in treated quadriceps was extrachromosomal. If integration had occurred, the frequency was </= 1-8 integrations per 150,000 diploid cells, which would be at least three orders of magnitude below the spontaneous mutation rate. Our results suggest that the risk of mutation due to integration of plasmid DNA vaccines following intramuscular injection is negligible.
Firm IP development: methodology and deliverables (2002)

This paper addresses the need to develop firm intellectual properties (IPs) with a standard set of deliverables so they can be integrated with very little effort. We have presented the design flow used for developing a firm IP. A DMA controller is used as an example. The paper highlights the deliverables from a IP vendor/user perspective to proliferate the acceptance and usage of the IP.
Development of a LAG-3 immunohistochemistry assay for melanoma (2022)

We have demonstrated that the assay reliably assesses LAG-3 expression in FFPE human melanoma samples by IHC.
Purification of a Cysteine Proteinase Inhibitor from Rice,<i>Oryza sativa</i>L.<i>japonica</i> (1985)

Journal Article Purification of a Cysteine Proteinase Inhibitor from Rice, Oryza sativa L. japonica Get access Keiko Abe, Keiko Abe Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan Search for other works by this author on: Oxford Academic Google Scholar Soichi Arai Soichi Arai Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 49, Issue 11, 1 November 1985, Pages 3349–3350, https://doi.org/10.1080/00021369.1985.10867271 Published: 01 November 1985 Article history Received: 20 February 1985 Published: 01 November 1985
Tensile Properties of Large Extrusion and Forgings of RS P/M Al-9Fe-1.9Mo-1.7Si Aluminum Alloy (2007)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Flame colour characterization in the visible and infrared spectrum using a digital camera and image processing (2008)

An attempt has been made to characterize the colour spectrum of methane flame under various burning conditions using RGB and HSV colour models instead of resolving the real physical spectrum. The results demonstrate that each type of flame has its own characteristic distribution in both the RGB and HSV space. It has also been observed that the averaged B and G values in the RGB model represent well the CH* and C*2 emission of methane premixed flame. Theses features may be utilized for flame measurement and monitoring. The great advantage of using a conventional camera for monitoring flame properties based on the colour spectrum is that it is readily available, easy to interface with a computer, cost effective and has certain spatial resolution. Furthermore, it has been demonstrated that a conventional digital camera is able to image flame not only in the visible spectrum but also in the infrared. This feature is useful in avoiding the problem of image saturation typically encountered in capturing the very bright sooty flames. As a result, further digital imaging processing and quantitative information extraction is possible. It has been identified that an infrared image also has its own distribution in both the RGB and HSV colour space in comparison with a flame image in the visible spectrum. © 2008 IOP Publishing Ltd.
A 1D time-varying median filter for seismic random, spike-like noise elimination (2008)

Abstract Random noise in seismic data affects the signal-to-noise ratio, obscures details, and complicates identification of useful information. We have developed a new method for reducing random, spike-like noise in seismic data. The method is based on a 1D stationary median filter (MF) — the 1D time-varying median filter (TVMF). We design a threshold value that controls the filter window according to characteristics of signal and random, spike-like noise. In view of the relationship between seismic data and the threshold value, we chose median filters with different time-varying filter windows to eliminate random, spike-like noise. When comparing our method with other common methods, e.g., the band-pass filter and stationary MF, we found that the TVMF strikes a balance between eliminating random noise and protecting useful information. We tested the feasibility of our method in reducing seismic random, spike-like noise, on a synthetic dataset. Results of applying the method to seismic land data from Texas demonstrated that the TVMF method is effective in practice.
Bioactive and bioresorbable cellular cubic-composite scaffolds for use in bone reconstruction (2006)

We used a novel composite fibre-precipitation method to create bioactive and bioresorbable cellular cubic composites containing calcium phosphate (CaP) particles (unsintered and uncalcined hydroxyapatite (u-HA), alpha-tricalcium phosphate, beta-tricalcium phosphate, tetracalcium phosphate, dicalcium phosphate dihydrate, dicalcium phosphate anhydrate or octacalcium phosphate) in a poly-D/L-lactide matrix. The CaP particles occupied greater than or equal to 70 wt% (greater than or equal to 50 vol%) fractions within the composites. The porosities of the cellular cubic composites were greater than or equal to 70% and interconnective pores accounted for greater than or equal to 70% of these values. In vitro changes in the cellular geometries and physical properties of the composites were evaluated over time. The Alamar Blue assay was used to measure osteoblast proliferation, while the alkaline phosphatase assay was used to measure osteoblast differentiation. Cellular cubic C-u-HA70, which contained 70 wt% u-HA particles in a 30 wt% poly-D/L-lactide matrix, showed the greatest three-dimensional cell affinity among the materials tested. This composite had similar compressive strength and cellular geometry to cancellous bone, could be modified intraoperatively (by trimming or heating) and was able to form cortico-cancellous bone-like hybrids. The osteoinductivity of C-u-HA70, independent of biological growth factors, was confirmed by implantation into the back muscles of beagles. Our results demonstrated that C-u-HA70 has the potential as a cell scaffold or temporary hard-tissue substitute for clinical use in bone reconstruction.
The contribution of thermoreflectance to high resolution thermal mapping (2003)

Thermal phenomena that occur in operating integrated circuits can disturb their functioning and even cause failures. In order to prevent such dramatic issues, it is necessary to study these phenomena by developing high‐resolution thermal mapping of electronic devices. This can be done by using the thermoreflectance technique. The principle of thermoreflectance measurements is reviewed and various experimental setups are described. Experimental results show that this technique allows the mapping of both low and high frequency thermal phenomena at submicron scales.
Soybean Phosphatides in Prothrombin-Consumption and Thromboplastin-Generation Tests: Their Use in Recognizing “Thrombasthenic Hemophilia” (1960)

Journal Article Soybean Phosphatides in Prothrombin-Consumption and Thromboplastin-Generation Tests: Their Use in Recognizing “Thrombasthenic Hemophilia” Get access Charles A. Owen, Jr., M.D., Charles A. Owen, Jr., M.D. Section of Clinical Pathology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota Search for other works by this author on: Oxford Academic Google Scholar John H. Thompson, Jr., Ph.D. John H. Thompson, Jr., Ph.D. Section of Clinical Pathology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 33, Issue 3, 1 March 1960, Pages 197–208, https://doi.org/10.1093/ajcp/33.3.197 Published: 01 March 1960
PPFMs and Other Covert Contaminants: Is There More to Plant Physiology Than Just Plant? (1994)

Crop loss due to soil salinization is an increasing threat to agriculture worldwide. This review provides an overview of cellular and physiological mechanisms in plant responses to salt. We place cellular responses in a time- and tissue-dependent context ...Read More
A dynamic modeling and a fault detection scheme of a PMSM under an inter turn short (2012)

A PMSM stator inter turn short fault generates a back emf and an impedance unbalance. Those unbalance causes a torque ripple and an escalating partial heat defect. This paper proposes a fault detection scheme based on the PMSM dynamic model under the inter turn short which is derived in the positive and the negative sequence synchronous reference frames. To validate the proposed scheme, the finite elements method (FEM) with electric circuit is conducted on the PMSM.
Tyrosine Phosphorylation of Lactate Dehydrogenase A Is Important for NADH/NAD <sup>+</sup> Redox Homeostasis in Cancer Cells (2011)

The Warburg effect describes an increase in aerobic glycolysis and enhanced lactate production in cancer cells. Lactate dehydrogenase A (LDH-A) regulates the last step of glycolysis that generates lactate and permits the regeneration of NAD(+). LDH-A gene expression is believed to be upregulated by both HIF and Myc in cancer cells to achieve increased lactate production. However, how oncogenic signals activate LDH-A to regulate cancer cell metabolism remains unclear. We found that the oncogenic receptor tyrosine kinase FGFR1 directly phosphorylates LDH-A. Phosphorylation at Y10 and Y83 enhances LDH-A activity by enhancing the formation of active, tetrameric LDH-A and the binding of LDH-A substrate NADH, respectively. Moreover, Y10 phosphorylation of LDH-A is common in diverse human cancer cells, which correlates with activation of multiple oncogenic tyrosine kinases. Interestingly, cancer cells with stable knockdown of endogenous LDH-A and rescue expression of a catalytic hypomorph LDH-A mutant, Y10F, demonstrate increased respiration through mitochondrial complex I to sustain glycolysis by providing NAD(+). However, such a compensatory increase in mitochondrial respiration in Y10F cells is insufficient to fully sustain glycolysis. Y10 rescue cells show decreased cell proliferation and ATP levels under hypoxia and reduced tumor growth in xenograft nude mice. Our findings suggest that tyrosine phosphorylation enhances LDH-A enzyme activity to promote the Warburg effect and tumor growth by regulating the NADH/NAD(+) redox homeostasis, representing an acute molecular mechanism underlying the enhanced lactate production in cancer cells.
Development of a Calorimeter to Measure the Self-Heating Characteristics of Coal (1985)

A recently developed calorimeter is described which measures the total heat produced by a one kilogram sample of coal under controlled oxidizing conditions. Essentially the calorimeter measures the maximum temperature rise produced by a sample of coal in a dynamic oxygen atmosphere which is held at predetermined constant moisture content. The observed temperature rise is proportional to the heat generated during the initiation of the oxidation process and is measured by a series of thermistors situated in the coal. The magnitude of this temperature rise effectively determines the self heating propensity of the coal. The sample compartment of this apparatus is designed to eliminate any thermal gradients within the coal sample which may lead to non-uniform temperature rises. This apparatus enables the reproducible measurement of a coal's self heating propensity, in terms of time-temperature curves, to be made under different environmental conditions of humidity, temperature and oxygen content. The calorimeter has been used to establish the role, in the oxidation process, of moisture, initial temperature, air flow rate and size distribution. Experimental evidence indicates that moisture acts as a contact catalyst in the oxidation of coal.
Human pharmacokinetics and disposition of sarmoxicillin, a lipophilic amoxicillin prodrug (1981)

Sarmoxicillin, an amoxicillin prodrug, is the methoxymethyl ester of hetamoxicillin. Esterification converted amoxicillin from an amphoteric to a cationic compound and resulted in a 30- to 600-fold increase in lipid partitioning. Oral absorption studies in normal subjects demonstrated that sarmoxicillin was only partially hydrolyzed by nonenzymatic and gut or hepatic first-pass metabolism and that significant quantities of intact ester appeared in the systemic circulation. Sarmoxicillin was converted to amoxicillin in plasma by hydrolysis of the acetone penicinate and the methoxymethyl ester bonds. Significant amoxicillin levels were demonstrated in saliva after administration of sarmoxicillin, but not amoxicillin, over a 250- to 1,000-mg dose range. Differences in the absorption, distribution, or metabolism of amoxicillin were also evident in the lower plasma amoxicillin maximum concentration and area under the curve and longer half-life after sarmoxicillin administration. Differences in the distribution of this lipophilic ester could result in a significant increase in tissue penetration and subsequent therapeutic efficacy of amoxicillin when administered as sarmoxicillin.
Influence of melatonin supplementation on serum antioxidative properties and impact of the quality of life in multiple sclerosis patients. (2014)

The relationship between the prevalence of multiple sclerosis (MS) and sunlight's ultraviolet radiation was proved. Oxidative stress plays a role in the pathogenic traits of MS. Melatonin possesses antioxidative properties and regulates circadian rhythms. Several studies have reported that the quality of life is worse in patients with MS than in healthy controls, with a higher prevalence of sleep disturbances, depression and fatigue. The aim of study was to evaluate 5 mg daily melatonin supplementation over 90 days on serum malondialdehyde (MDA) concentration and superoxide dismutase (SOD) activity and its' influence on impact of the quality of life of MS patients. A case-control prospective study was performed on 102 MS patients and 20 controls matched for age and sex. The EDSS, MRI examinations and Multiple Sclerosis Impact Scale (MSIS-29) questionnaire was completed. Marked increase in serum MDA concentration in all MS patients groups was observed and after melatonin treatment decreased significantly in interferons-beta and glatiramer acetate-treated groups, but not in mitoxantrone-treated group. A significant increase in SOD activity compared to controls only in glatiramer acetate-treated group was observed. After 3 months melatonin supplementation the SOD activity increased compared to initial values in interferons beta-treated groups. A significant increase in both MSIS-29-PHYS and MSIS-29-PSYCH items mean scores only in the MX group as compared to other groups was observed. There were no significant differences in mean MSIS-29-PHYS was observed before and after melatonin therapy. Melatonin supplementation caused a decrease in mean MSIS-29-PSYCH scores compared to initial values in interferons beta-treated groups. Finding from our study suggest that melatonin can act as an antioxidant and improves reduced quality in MS patients.
Enzymes as denture cleansers (1977)

It was the purpose of the study to test the efficiency of dextranase, mutanase, and protease in removing denture plaque. The study group comprised 100 denture-wearers with denture stomatitis. The enzymes were dispensed as dissolvent tablets either in pure or in mixed preparations. Placebo tablets and Steradent, a commercial denture cleanser, were used as control tablets. The following parameters were studied: the amount of denture plaque, the degree of palatal erythema, and the concentration of yeast cells and inflammatory cells in mucosal and denture smears. The study was designed and carried out as a double-blind study. The dissolvent tablets containing the enzymes in mixed preparations were more effective than the tablets containing the pure enzymes, the placebo tablets, or the Steradent tablets. The beneficial effect of the mixed enzyme preparations included a significant reduction of the amount of denture plaque and an improvement of the clinical condition of the palatal mucosa.
Type I Interferon Induces Inhibitory 16-kD CCAAT/ Enhancer Binding Protein (C/EBP)β, Repressing the HIV-1 Long Terminal Repeat in Macrophages: Pulmonary Tuberculosis Alters C/EBP Expression, Enhancing HIV-1 Replication (1998)

We have previously observed that HIV-1 replication is suppressed in uninflamed lung and increased during tuberculosis. In vitro THP-1 cell–derived macrophages inhibited HIV-1 replication after infection with Mycobacterium tuberculosis. Suppression of HIV-1 replication was associated with inhibition of the HIV-1 long terminal repeat (LTR) and induction of ISGF-3, a type I interferon (IFN)–specific transcription factor. Repression of the HIV-1 LTR required intact CCAAT/enhancer binding protein (C/EBP) sites. THP-1 cell–derived macrophages infected with M. tuberculosis, lipopolysaccharide, or IFN-β induced the 16-kD inhibitory C/EBPβ isoform and coincidentally repressed HIV-1 LTR transcription. C/EBPβ was the predominant C/EBP family member produced in THP-1 macrophages during HIV-1 LTR repression. In vivo, alveolar macrophages from uninflamed lung strongly expressed inhibitory 16-kD C/EBPβ, but pulmonary tuberculosis abolished inhibitory C/EBPβ expression and induced a novel C/EBP DNA binding protein. Therefore, in vitro, proinflammatory stimulation produces an IFN response inhibiting viral replication by induction of a C/EBPβ transcriptional repressor. THP-1 cell–derived macrophages stimulated with type I IFN are similar to alveolar macrophages in the uninflamed lung in vivo. In contrast, the cellular immune response in active pulmonary tuberculosis disrupts this innate immunity, switching C/EBP expression and allowing high level viral replication.
Dual-structure Data Center Multicast Using Software Defined Networking (2014)

Data center applications use multicast as an effective method to reduce bandwidth cost. However, traditional multicast protocols designed for IP networks are usually bottlenecked by the limited state capacity on switches. In this paper, we propose a scalable multicast solution on fat tree networks based on the observation that data center multicast traffic has strong heterogeneity. We propose to remove the multicast management logic from switches and use the SDN controller to manage multicast groups. The proposed Dual-structure Multicast (DuSM) determines elephant and mice groups according to their traffic amounts and treats them separately. For each elephant group, the controller installs multicast state to maintain multiple shared trees and the group traffic will be balanced evenly among the trees to avoid congestion. For mice groups, the controller applies state-free mutlicast that trades bandwidth capacity for state capacity, such as multicast-to-unicast translation. Our experiments using real multicast traffic data show that the number of groups DuSM supports can be 300% of that of IP multicast. DuSM also achieves traffic balance among links.
Effect of blocking VEGF, hTERT and Bcl-xl by multiple shRNA expression vectors on the human laryngeal squamous carcinoma xenograft in nude mice (2008)

Our results demonstrate that the application of vector-based RNAi technology that involves blocking multiple targets will be a promising therapeutic modality in the gene therapy of human cancers. The data strongly suggest that simultaneously blocking multiple genes in human cancers using an RNAi approach should be considered in cancer therapy.
Spatial sound localization in an augmented reality environment (2006)

Augmented Reality (AR), the overlay of virtual images onto the real world, is an increasingly popular technique for developing new human-computer interfaces. As human navigation and orientation in different environments depend on both visual and auditory information, sound plays a very important role in AR applications. In this paper we explore users' capability to localize a spatial sound (registered with a virtual object) in an AR environment, under different spatial configurations of the virtual scene. The results not only confirm several previous findings on sound localization, but also point out some important new visual-audio cues which should be taken into consideration for effective localization and orientation in AR environment. Finally, this paper provides tentative guidelines for adding spatial sound to AR environments.