gebhart/patrepeval huggingface dataset:
Characterization of Intrastriatal Recombinant Adeno-Associated Virus-Mediated Gene Transfer of Human Tyrosine Hydroxylase and Human GTP-Cyclohydrolase I in a Rat Model of Parkinson’s Disease (1998)

To achieve local, continuous l -DOPA delivery in the striatum by gene replacement as a model for a gene therapy for Parkinson’s disease, the present studies used high titer purified recombinant adeno-associated virus (rAAV) containing cDNAs encoding human tyrosine hydroxylase (hTH) or human GTP-cyclohydrolase I [GTPCHI, the rate-limiting enzyme for tetrahydrobiopterin (BH 4 ) synthesis] or both to infect the 6-OHDA denervated rat striatum. Striatal TH and GTPCHI staining was observed 3 weeks after rAAV transduction, with little detectable perturbation of the tissue. Six months after intrastriatal rAAV transduction, TH staining was present but apparently reduced compared with the 3 week survival time. In a separate group of animals, striatal TH staining was demonstrated 1 year after rAAV transduction. Double staining studies using the neuronal marker NeuN indicated that >90% of rAAV-transduced cells expressing hTH were neurons. Microdialysis experiments indicated that only those lesioned animals that received the mixture of MD–TH and MD–GTPCHI vector displayed BH 4 independent in vivo l -DOPA production (mean ∼4–7 ng/ml). Rats that received the hTH rAAV vector alone produced measurable l -DOPA (mean ∼1–4 ng/ml) only after receiving exogenous BH 4 . l -Aromatic amino acid decarboxylase blockade, but not 100 m m KCl-induced depolarization, enhanced l -DOPA overflow, and animals in the non-hTH groups (GTPCHI and alkaline phosphatase) yielded minimal l -DOPA. Although elevated l -DOPA was observed in animals that received mixed hTH and hGTPCHI rAAV vectors, there was no reduction of apomorphine-induced rotational behavior 3 weeks after intrastriatal vector injection. These data demonstrate that purified rAAV, a safe and nonpathogenic viral vector, mediates long-term striatal hTH transgene expression in neurons and can be used to successfully deliver l -DOPA to the striatum.
THE ANTICANCER AGENT PB-100 CONCENTRATES IN THE NUCLEUS AND NUCLEOLI OF HUMAN GLIOBLASTOMA CELLS BUT DOES NOT ENTER NORMAL ASTROCYTES (1995)

Selectivity of the anticancer agent PB-100 for malignant cells, already demonstrated using cell growth and viability evaluation, is now confirmed by microscopic observations. PB-100 is easily detected inside cells by its yellow color under visible light and by its blue fluorescence; it may be measured in isolated nuclei using its characteristic UV absorbance. After short treatment of human BCNU-resistant glioblastoma cells (U 251) and normal astrocyte controls (CRL 1656), PB-100 accumulates in the malignant cell nucleus, particularly concentrating in the multiple nucleoli and rapidly inducing glioblastoma cell death, whilst, in contrast, the anticancer agent does not even enter normal cells. We had already shown that PB-100 binds to DNA of cancer cells, but not to that of normal cells. In vitro tests described in this report indicate that PB-100 binds to purine bases, but not to pyrimidines, of various ribopolymers and its binding to purine rich nucleic acid stretches is inferred.
Niridazole Suppression of Experimental Allergic Encephalomyelitis in Lewis Rats (1977)

Administration of niridazole to Lewis rats beginning 2 days before sensitization with guinea pig spinal cord in combination with immunologic adjuvants exerted a dose related suppressive influence on development of experimental allergic encephalomyelitis (EAE). A daily dose of 75 mg/kg completely prevented clinical neurologic signs as well as markedly suppressed occurrence of immunohistopathologic manifestations of this autoimmune disease. A higher daily dose level of niridazole, i.e., 100 mg/k, also inhibited EAE but was associated with neurotoxic manifestations.
Cerebral Vascular Hamartomas in Five Dogs (2001)

Vascular hamartomas are considered developmental lesions rather than true neoplasms. Reports of such anomalies in the canine brain are scarce, and their classification is confusing. This case series of vascular hamartomas from the brains of five dogs was characterized using histochemistry and immunohistochemistry, in addition to gross and microscopic findings. All five hamartomas were located in the telencephalon, three in the pyriform lobe, without any predilection for the left or right side. Each hamartoma consisted of a proliferation of thin-walled vessels which varied in caliber. These vessels were elastin-negative, with varying amounts of collagen and no muscular component. In four of the five hamartomas, lining cells were actin- and factor VIII-positive. All five hamartomas contained glial fibrillary acid protein (GFAP)-positive parenchyma at moderate to high frequency, and four contained neurofilament-positive axons between component vessels. This report shows that vascular hamartomas in the canine brain are structural malformations for which immunohistochemistry is useful for accurate classification.
Fabrication of All-Nb Josephson Junctions Using Oxidized Zr Overlayers (1986)

Fabrication of all-Nb Josephson tunnel junctions using artificial barriers of oxidized Zr overlayers is described. Thin Zr overlayers are sputter-deposited onto polycrystalline Nb films and then thermally oxidized. The tunneling characteristics of these junctions are found to be greatly affected by the occurrence of Zr diffusion into the grain boundaries of the base Nb films during Zr deposition, which is confirmed by XPS analysis. Paying particular attention to the rapid substrate cooling, high quality junctions with V m =47 mV are obtained.
Partial Regeneration/Proliferation of the .BETA.-Cells in the Islets of Langerhans by Nigella sativa L. in Streptozotocin-Induced Diabetic Rats (2003)

This experiment was carried out to investigate the effect of N. sativa L. on histopathology of pancreatic beta-cells, and blood insulin and glucose concentrations in streptozotocin-induced diabetic rats. Fifty male Wistar rats (200-250 g) were divided into two experimental groups (diabetics with no treatment and diabetics with N. sativa L. treatment), each containing twenty-five rats. Diabetes was induced in both groups by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). The experimental animals in both groups became diabetic within 24 hours after the administration of STZ. The rats in N. sativa L.-treated group were given the daily intraperitoneal injection of 0.20 ml/kg of N. sativa L. volatile oil for 30 days starting the day after STZ injection. Control rats received only the same amount of normal saline solution. The rats in both groups received the last injection 24 hours before the sacrification and 5 randomly-selected rats in each group were sacrificed before, and the 1, 10, 20 and 30 days after the STZ injection to collect blood and pancreatic tissue samples. The N. sativa L. treatment caused a decrease in the elevated serum glucose, an increase in the lowered serum insulin concentrations and partial regeneration/ proliferation of pancreatic beta-cells in STZ-induced diabetic rats with the elapse of the experiment. It is concluded that the hypoglycaemic action of N. sativa L. could be partly due to amelioration in the beta-cells of pancreatic islets causing an increase in insulin secretion. More studies are needed to demonstrate the exact mechanism of action of N. sativa L. on ameliorated blood glucose concentration in STZ-induced diabetes.
A framework of fuzzy modeling using genetic algorithms with appropriate combination of evaluation criteria (2002)

Fuzzy modeling is a method to describe nonlinear input-output relationships. Genetic algorithms (GAs) have been used with fuzzy modeling for identification of the structure of a fuzzy model and selection of input variables. Users often require fuzzy models that satisfy multiple evaluation criteria. Assignment of appropriate weights on these criteria is one of the key factors for good GA search. In order to give a guideline for assigning the degree of importance to each evaluation criterion for generating fuzzy models, we examined the characteristic of each evaluation criterion.
In vitro activity of midecamycin, a new macrolide antibiotic (1983)

Midecamycin, an acetoxy-substituted macrolide antibiotic, was tested against gram-positive and gram-negative bacteria. It inhibited the majority of streptococci, staphylococci, and strains of Haemophilus and Listeria at concentrations of less than 3.1 micrograms/ml. It was less active than erythromycin, and it failed to inhibit erythromycin-resistant isolates.
Developing a cyber threat intelligence sharing platform for South African organisations (2017)

Cyberattacks are on the increase in severity, complexity and frequency, negatively affecting the citizens, government, and businesses. Adversely, the security and Defence role-players in developing countries, such as South Africa, are short of the required capacity and capability to adequately defend and protect the national cyberspace against these fast moving and persistent threats and attacks. Be that as it may, the South African cyberspace still requires national attention and protection by the mandated role-players, such as the Defence force and its industry partners. Thus, within the cyber domain, the various Defence force role-players can no longer rely on traditional solutions to detect, defend, and respond to the forever changing cyber threats and cyberattacks. In order to reduce cybersecurity risks and strengthen cyber resilience of the nation, strategic cybersecurity information sharing in the Defence environment is becoming a necessity. Thus, the contribution from this paper is a systematic discussion and demonstration of a conceptual cyber threat intelligence sharing model and platform that could stimulate and enable different stakeholders within the Defence environment to seamlessly and collaboratively aggregate, analyse, and timely share contextual and actionable cyber-threat intelligence that could lead to a resilient cybersecurity posture and better protection of the national cyberspace.
Prodigiosin<sup>1a</sup> (1963)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProdigiosin1aA. J. Castro, J. F. Deck, M. T. Hugo, E. J. Lowe, J. P. Marsh Jr., and R. J. PfeifferCite this: J. Org. Chem. 1963, 28, 3, 857–860Publication Date (Print):March 1, 1963Publication History Published online1 May 2002Published inissue 1 March 1963https://pubs.acs.org/doi/10.1021/jo01038a508https://doi.org/10.1021/jo01038a508research-articleACS PublicationsRequest reuse permissionsArticle Views179Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Retinoic Acid Leads to Cytoskeletal Rearrangement through AMPK-Rac1 and Stimulates Glucose Uptake through AMPK-p38 MAPK in Skeletal Muscle Cells (2008)

Retinoic acid (RA) is one of the major components of vitamin A. In the present study, we found that retinoic acid activated AMP-activated protein kinase (AMPK). RA induced Rac1-GTP formation and phosphorylation of its downstream target, p21-activated kinase (PAK), whereas the inhibition of AMPK blocked RA-induced Rac1 activation. Moreover, cofilin, an actin polymerization regulator, was activated when incubated with RA. We then showed that inhibition of AMPK by compound C, a selective inhibitor of AMPK, or small interfering RNA of AMPK alpha1 blocked RA-induced cofilin phosphorylation. Additionally, we found that retinoic acid-stimulated glucose uptake in differentiated C2C12 myoblast cells and activated p38 mitogen-activated protein kinase (MAPK). Finally, the inhibition of AMPK and p38 MAPK blocked retinoic acid-induced glucose uptake. In summary, our results suggest that retinoic acid may have cytoskeletal roles in skeletal muscle cells via stimulation of the AMPK-Rac1-PAK-cofillin pathway and may also have beneficial roles in glucose metabolism via stimulation of the AMPK-p38 MAPK pathway.
Analysis of Vinylene Carbonate Derived SEI Layers on Graphite Anode (2004)

The solid electrolyte interface (SEI) formation on composite graphite and highly oriented pyrolytic graphite in a vinylene carbonate (VC)-containing electrolyte was analyzed using evolved gas analysis, Fourier transform infrared analysis, two-dimensional nuclear magnetic resonance, X-ray photoelectron spectroscopy, time of flight-secondary-ion mass spectrometry, and scanning electron microscopy. We found that the SEI layers derived from VC-containing electrolytes consist of polymer species such as poly (vinylene carbonate) (poly(VC)), an oligomer of VC, a ring-opening polymer of VC, and polyacetylene. Moreover, lithium vinylene dicarbonate, lithium divinylene dicarbonate, lithium divinylene dialkoxide, and lithium carboxylate, RCOOLi, were formed on graphite as VC reduction products. The presence of VC in the ethylene carbonate (EC)-based electrolyte caused a decrease in the reductive gases of the EC dimethyl carbonate solvent such as and CO. The VC-derived SEI layer was formed at a potential more positive than 1.0 V vs. Effective SEI formation by reduction of VC progresses before that of EC. The thermal decomposition temperature of the SEI layer derived from VC shifted to a higher temperature compared to that derived from the VC-free electrolytes. We concluded that the thermal stability of the VC-derived SEI layer has a close relation to high-temperature storage characteristics at elevated temperatures. © 2004 The Electrochemical Society. All rights reserved.
High gradient induction accelerator (2007)

A new type of compact induction accelerator is under development at the Lawrence Livermore National Laboratory that promises to increase the average accelerating gradient by at least an order of magnitude over that of existing induction machines. The machine is based on the use of high gradient vacuum insulators, advanced dielectric materials and switches and is stimulated by the desire for compact flash X-ray radiography sources. Research describing an extreme variant of this technology aimed at proton therapy for cancer will be described. Progress in applying this technology to several applications will be reviewed.
Is State Law Looking for Trouble: The Federal Arbitration Act Flexes Its Preemptive Muscle (2003)

This article begins with an overview of the preemption concept as it affects the American legal system. The source of preemption power is revealed and the most common forms of preemption are introduced. Next, the article discusses preemption and its interaction with the Federal Arbitration Act (FAA). The discussion begins with a chronological view of the cases that have defined the effects the FAA has on arbitration agreements via its preemption power and ends with a summary of the current state of the law.
Beacon Technologies (2016)

Proximity technologyâin particular, Bluetooth beaconsâis a major source of business opportunity, and this book provides everything you need to know to architect a solution to capitalize on that opportunity. Learn the key standardsâiBeacon, Eddystone, Bluetooth 4.0, and AltBeaconâand how they work with other proximity technologies. Then build your understanding of the proximity framework and how to identify and deploy the best solutions for your own business, institutional, or consulting needs. Solutions architects of all typesâventure capitalists, founders, CEOs, strategists, product managers, CTOs, business developers, and programmersâwill learn about the following from reading Beacon Technologies: The Hitchhiker's Guide to the Beacosystem: â¢The disruptive implications of digitalâphysical convergence and the new applications it makes possible â¢The key standards that solutions developers need to understand to capitalize on the business opportunity of proximity technology â¢The new phenomenon of beacon networks, which will be hugely significant in driving strategic decisions and creating wealth â¢Other technologies in the proximity ecosystem catalyzed by and complementary to Bluetooth beacons, including visual light communication, magnetic resonance, and RFID â¢The Beacosystem framework for analyzing the proximity ecosystem Steve Statler is a writer, public speaker, and consultant working in the beacon ecosystem. He trains and advises retailers, venue owners, VCs, as well as makers of beacon software and hardware. Previously he was the Senior Director for Strategy and Solutions Management at Qualcomm's Retail Solutions Division, helping to incubate Gimbal, one of the leading Bluetooth beacons on the market. He is also the CEO of Cause Based Solutions, creators of Give the Change, democratizing philanthropy, enabling non-profit supporters to donate the change from charity branded debit cards, and developer of The Good Traveler program. Contributors: Anke Audenaert, CEO, Favrit John Coombs, CEO, Rover Labs Theresa Mary Gordon, Co-Founder, tapGOconnect Phil Hendrix, Director, immr Kris Kolodziej, President, IndoorLBS Patrick Leddy, CEO, Pulsate Ben Parker, VP Business Development, AccelerateIT Mario Proietti, CEO, Location Smart Ray Rotolo, SVP OOH, Gimbal Kjartan Slette, COO, Unacast Jarno Vanto, Partner, Borenius Attorneys LLP David Young, Chief Engineer, Radius Networks Foreword by Asif Khan, President LBMA.
Novel approaches toward ninhydrin analogs (1994)

Several new 5-arylninhydrins have been prepared using palladium-catalyzed cross-coupling reactions.
Effect of mechanical means on the restoration of the limb regenerative ability in metamorphic stages of Bufo regularis Reuss. (1975)

Limbs of Bufo regularis REUSS metamorphic stages No. 57, 63 and 66 were transected at the thigh level. Removal of the apical skin cover on the third postoperative day, followed by traumatization after further two days in the limb stumps, was more effective in the enhancement of the regenerative ability than when each one of these procedures was performed independently. Better results were achieved in cases of the preclimactic stage (No. 57) where about 30% of the cases (Series III) developed heteromorphic limb outgrowths having more distal structures as the shank ending with or without toes. Normally, after transection at the same level, the limb stumps of this stage could restore part of the thigh region only.
Shape representation using space filled sub-voxel distance fields (2002)

Voxelisation is the process of converting a source object of any data type into a three-dimensional grid of voxel values. This voxel grid should represent the original object as closely as possible, although some inaccuracies will occur due to the discrete nature of the voxel grid representation. In this paper we report our ongoing research into methods for representing objects as voxelised distance fields, in particular we report fast methods for accurate distance field production. A review of current alternative voxelisation methods is also given.
The role of transcription factors of neurosensory cells in non-syndromic sensorineural hearing loss with or without inner ear malformation (2015)

Only the c.133A > G (p. Ala45Thr) in the Neurod1 gene was detected in this study. The allele frequencies of this variant were 88.00% and 84.88% in the inner ear malformation group and the normal inner ear group, respectively, while 90.85% of children in the control group carried c.133A > G. This variant existed in every group commonly and had no significant difference among them. No variant in the other two TFs was detected in this cohort.
A New Facile, and Convenient Synthesis of 2-Oxo-3-alkenoic Acids and Esters (1986)

Activated 1-alkenes such as ketene dithioacetals, vinyl sulfides, N-vinyl amides, and N-vinylcarbazole react with oxalyl chloride or ethoxalyl chloride in the presence of pyridine to give the 2-oxo-3-alkenoic acids or ethyl esters, respectively.
PCAN: 3D Attention Map Learning Using Contextual Information for Point Cloud Based Retrieval (2019)

Point cloud based retrieval for place recognition is an emerging problem in vision field. The main challenge is how to find an efficient way to encode the local features into a discriminative global descriptor. In this paper, we propose a Point Contextual Attention Network (PCAN), which can predict the significance of each local point feature based on point context. Our network makes it possible to pay more attention to the task-relevent features when aggregating local features. Experiments on various benchmark datasets show that the proposed network can provide outperformance than current state-of-the-art approaches.
Highly efficient photorefractive polymers for dynamic holography (1995)

The performance of recently developed highly efficient photoref_ ract1ve polyme_rs .base~ on the photoconducting polymer host poly( N-vinylcarbazole) 1s 1nvest1gated, and the use of these materials as recording media in dynamic holography and other applications is evaluated.- A diffraction efficiency 'rJ = 86% (limited only by absorption and reflection losses), a two-beam coupling net gain coefficient r = 200 cm - 1 and light-induced refractive index modulations as high as lin=7X 10-3 are demonstrated. Hologram growth rates of the order of 500 ms are observed with. recording light intensities <10 mW /cm2 , using either lowpower laser diodes (675 nm) or a HeNe laser (633 nm). The materials show good sensitivity in this part of the spectrum. Applications such as dynamic time-average interferometry are demonstrated.
Comparison of Leukocytic Pyrogen and Leukocytic Endogenous Mediator (1977)

SummaryLeukocytic pyrogen was subjected to a five-step purification scheme, and quantitative assays for both leukocytic pyrogen and leukocytic endogenous mediator (LEM) were made. At each step of the purification scheme, the active fractions, in addition to producing fever, lowered plasma iron and zinc concentrations, elevated fi-brinogen concentrations, and caused a release of neutrophils from bone marrow. It appears, therefore, that LEM and leukocytic pyrogen are the same molecule.