gebhart/patrepeval huggingface dataset:
Cloning and expression in Escherichia coli of histidine utilization genes from Pseudomonas putida (1985)

A library of the Pseudomonas putida chromosome, prepared through the use of the cosmid pJB8 ligated to a partial Sau3A digest of bacterial DNA, followed by in vitro packaging into bacteriophage lambda particles, was used to construct a strain of Escherichia coli which contained the genes for histidine utilization. This isolate produced a repressor product and all five enzymes required in Pseudomonas spp. for histidine dissimilation, whereas none of these could be detected in the nontransduced parent E. coli strain. When this transductant was grown on various media containing histidine or urocanate as the inducer, it was observed that production of the cloned histidine degradative enzymes was influenced somewhat by the choice of nitrogen source used but not by the carbon source. The recombinant cosmid was isolated and found to consist of 21.1 kilobase pairs of DNA, with approximately 16 kilobase pairs derived from Pseudomonas DNA and the remainder being from the pJB8 vector. Digestion of this insert DNA with EcoRI provided a 6.1-kilobase-pair fragment which, upon ligation in pUC8 and transformation into an E. coli host, was found to encode histidine ammonia-lyase and urocanase. The inducible nature of this production indicated that the hut repressor gene also was present on this fragment. Insertional inactivation of the histidine ammonia-lyase and urocanase genes by the gamma-delta transposon has permitted location of these structural genes and has provided evidence that transcription proceeds from urocanase through histidine ammonia-lyase. Mapping of the 16-kilobase-pair Pseudomonas DNA segment with restriction enzymes and subcloning of additional portions, one of which contained the gene for formiminoglutamate hydrolase and another that could constitutively express activities for both imidazolone propionate hydrolase and formylglutamate hydrolase, has provided evidence for the organization of all hut genes.
Digital circuit capacitance and switching analysis for ground bounce in ICs with a high-ohmic substrate (2004)

Substrate noise is a major obstacle for mixed-signal integration. Ground bounce is a major contributor to substrate noise generation due to the resonance caused by the inductance and the Vdd-Vss admittance that consists of the on-chip digital circuit capacitance of the MOS transistors, the decoupling, and the parasitics arising from the interconnect. In this paper, we address: 1) the dependence of the Vdd-Vss admittance on the different states of the circuit, the supply voltage, and the interconnect, and 2) the computation of the total supply current with ground bounce. By using a fast and accurate macromodeling approach, the Vdd-Vss admittances of several test circuits are computed with 2%-3% error relative to the values simulated from the complete SPICE level netlist, but several orders of magnitude faster in CPU time and with 10% maximum error relative to the measurements on a test ASIC fabricated in a 0.18-/spl mu/m CMOS process on a high-ohmic substrate with 18 /spl Omega//spl middot/cm resistivity. The measurements also show that this admittance mainly depends only on the connectivity of the gates to the supply rail rather than their connectivity among each other.
Optical fibres coated with a UV-curable material at a speed of 12 m/s (1984)

The letter reports the successful achievement of coating silica fibre at a rate of greater than 10 m/s. We draw 125 μm-diameter fibres from silica rods and preforms, and coated in-line with UV-curable material. All fibres were proof-tested at two different stress levels of 0.7 and 1.4 GN/m2 We also checked the transmission properties of the fibre. It is found that the optical properties of the fibre remain the same while the mechanical properties are comparable to those of the fibres coated at a low speed.
Polarization characteristics of resistively loaded orthogonal dipoles excited by ultra wideband signals (2003)

Polarization characteristics of the radiated electric field in free space at far distances from resistively loaded thin half-wave orthogonal dipoles excited by short pulses are investigated. It is shown that the radiated field can become circularly polarized in the broadside direction when the exciting input voltage is sinusoidal and the input to the x-axis dipole is the same as the input to the z-axis dipole but delayed by a quarter cycle. The Wu-King loading profile (see Wu, T.T. and King, R.W.P., IEEE Trans. Antennas and Propagation, vol.13, p.369-73, 1965) is considered here. These results are compared with that of the radiated field of unloaded orthogonal dipoles excited by finite cycles of sinusoids.
Reliability Study OF A NURSING FLOW SHEET (1975)

The flow sheet discussed in this article raises record-keeping from a mundane chore to a creative component of the health care system. But, say the authors, it does even more than that. By making sure that all nurses using it agree on procedure and term definitions, it can point up not only organizational strengths in which all may take pride, but also weaknesses which may be corrected by inservice educational programs. Although the flow sheet is described in the geriatric setting in which these authors work, its greater universality is readily apparent. Sally Cohn, M.S.N., is instructor of nursing, University of Connecticut, Storrs. Audrey Fulcher, R.N., M.A., is assistant professor of nursing, University of Connecticut. Nancy Gustafson, R.N., is geriatric nurse specialist, Hebrew Home for the Aged, Hartford, Connecticut. The authors would like to thank Dr. Ian Lawson, medical director of the Hebrew Home for the Aged, for his support during the conduct of this study. They also gratefully acknowledge Katherine Nuckolls, R.N., Ph.D., associate professor of nursing, Yale University School of Nursing, for her editorial assistance in the preparation of this article.
Cyanobacterial dominance: The role of buoyancy regulation in dynamic lake environments (1987)

Abstract The interactions of size, shape, and density of cyanobacteria result in a 5‐order of magnitude difference in flotation or sinking rates which, in turn, influence the extent of their dispersion in turbulent water masses. Active mixing through resource‐replete waters of high clarity favours fast‐growing, small‐celled species. Where photosynthetically active radiation is severely attenuated through the wind‐mixed layer, species may rely on turbulent entrainment but must be adapted toward efficient light harvesting (morphological attenuation, enhanced pigmentation). In both strongly segregated waters (light‐ and nutrient‐rich layers separated vertically) and waters experiencing high‐frequency fluctuations in vertical mixing and optical depth, emphasis is placed on the ability to make rapid, buoyancy‐adjusted vertical movements, favoured by large size. The cyanobacterial 1ife‐forms respectively typical of these contrasted limnological systems — unicellular coccoids (e.g., Synechococcus ), solitary filaments (e.g., Oscillatoria ) and colonial forms (e.g., Microcystis ) — illustrate the diversity of evolutionary adaptations to be discerned among the planktonic cyanobacteria and which contributes to their reputation as a prominent and successful group of organisms.
Compact binaries with code compression in a software dynamic translator (2004)

Embedded software is becoming more flexible and adaptable, which presents new challenges for management of highly constrained system resources. Software dynamic translation (SDT) has been used to enable software malleability at the instruction level for dynamic code optimizers, security checkers, and binary translators. This paper studies the feasibility of using SDT to manage program code storage in embedded systems. We explore to what extent code compression can be incorporated in a software infrastructure to reduce program storage requirements, while minimally impacting run-time performance and memory resources. We describe two approaches for code compression, called full and partial image compression, and evaluate their compression ratios and performance in a software dynamic translation system. We demonstrate that code decompression is indeed feasible in a SDT.
Nocturnal Fall of Blood Pressure and Silent Cerebrovascular Damage in Elderly Hypertensive Patients (1996)

To study the relation between diurnal blood pressure variations and silent cerebrovascular damage, we performed both 24-hour ambulatory blood pressure monitoring and brain magnetic resonance imaging in 131 elderly asymptomatic hypertensive patients. Silent cerebrovascular damage was identified by the magnetic resonance imaging findings of lacunae (low intensity in T1-weighted images and high intensity in T2-weighted images) and advanced periventricular hyperintense lesions (on T2-weighted images). The frequency of silent cerebrovascular damage in the 100 patients with sustained hypertension was greater than that in the 31 patients with white coat hypertension. We further classified the former group into nondippers (nocturnal reduction of systolic pressure by < 10% of awake systolic pressure; n = 46), dippers (reduction by > or = 10% to < 20%; n = 38), and extreme dippers (reduction by > or = 20%; n = 16). The extent of silent cerebrovascular damage was least severe in the dipper group (P < .05). This J-shaped relation was not found either with the cardiac hypertrophy detected by electrocardiography or with the renal damage assessed by urinary albumin excretion. More than half of the extreme dippers were patients with isolated systolic hypertension, and this prevalence was significantly greater than that in dippers or in nondippers (21% and 30%, respectively). Extreme dippers also had greater variability of pressure (standard deviation of awake systolic pressure) than dippers. Our results indicate that in addition to nondipping, extreme dipping (marked nocturnal fall of blood pressure) should be considered a type of abnormal diurnal blood pressure variation in elderly patients with hypertension who are likely to have advanced silent cerebrovascular damage.
Next generation micro-power systems (2008)

Emerging microsystems such as portable and implantable medical electronics, wireless microsensors and next-generation portable multimedia devices demand a dramatic reduction in energy consumption. The ultimate goal is to power these devices using energy harvesting techniques such as vibration-to-electric conversion or through wireless power transmission. A major opportunity to reduce the energy consumption of digital circuits is to scale supply voltages to 0.5V and below. The challenges associated with ultra-low-voltage design will be presented. These include variation-aware design for logic and SRAM circuits, efficient DC-DC converters for ultra-low-voltage delivery, and algorithm structuring to support extreme parallelism. This paper also addresses micro-power analog and RF circuits, which require the use of application-specific structures and highly digital variation-aware architectures.
Essai sur des directives de politique coloniale (1949)

La pagination indiquée dans la zone "Pages" est celle du tiré à part, numérisé par les Bibliothèques de l'ULB. La pagination, telle qu'elle apparaît dans la revue est pp. 23-38
Preliminary evidence that morning vaccination is associated with an enhanced antibody response in men (2008)

Variation in response to vaccination, particularly in vulnerable groups, provides a strong rationale for developing vaccine adjuvants. If there were consistent diurnal variation in immune response, this could inform a simple intervention for enhancing vaccine efficacy. Data from two studies are presented examining morning versus afternoon vaccine administration; in the first, hepatitis A vaccine was administered to young adults, and in the second, influenza vaccine to older community-based adults. Men, but not women, vaccinated in the morning mounted a better peak antibody response to both hepatitis A and the A/Panama influenza strain. These results indicate that it would be worthwhile testing this effect in a large randomized control trial with vaccination during time periods representing the extremes of hormonal and cytokine diurnal rhythms.
Extracting Dynamics from Static Cancer Expression Data (2008)

Static expression experiments analyze samples from many individuals. These samples are often snapshots of the progression of a certain disease such as cancer. This raises an intriguing question: Can we determine a temporal order for these samples? Such an ordering can lead to better understanding of the dynamics of the disease and to the identification of genes associated with its progression. In this paper we formally prove, for the first time, that under a model for the dynamics of the expression levels of a single gene, it is indeed possible to recover the correct ordering of the static expression datasets by solving an instance of the traveling salesman problem (TSP). In addition, we devise an algorithm that combines a TSP heuristic and probabilistic modeling for inferring the underlying temporal order of the microarray experiments. This algorithm constructs probabilistic continuous curves to represent expression profiles leading to accurate temporal reconstruction for human data. Applying our method to cancer expression data we show that the ordering derived agrees well with survival duration. A classifier that utilizes this ordering improves upon other classifiers suggested for this task. The set of genes displaying consistent behavior for the determined ordering are enriched for genes associated with cancer progression.
A multilevel sensing and program verifying scheme for Bi-HAND flash memories (2005)

A multi-level sensing and program verifying circuit is proposed for Bi-NAND type flash memories. The sensing circuit utilizes the advanced cross-coupled sense amplifier to achieve excellent immunity against mismatch effect, and reduce power consumption. The program-verifying scheme with dichotomous architecture simplifies the verifying circuit and speeds up verification process for multi-level cell arrays.
Elafin Is a Biomarker of Graft-Versus-Host Disease of the Skin (2010)

Graft-versus-host disease (GVHD), the major complication of allogeneic bone marrow transplantation, affects the skin, liver, and gastrointestinal tract. There are no plasma biomarkers specific for any acute GVHD target organ. We used a large-scale quantitative proteomic discovery procedure to identify biomarker candidates of skin GVHD and validated the lead candidate, elafin, with enzyme-linked immunosorbent assay in samples from 492 patients. Elafin was overexpressed in GVHD skin biopsies. Plasma concentrations of elafin were significantly higher at the onset of skin GVHD, correlated with the eventual maximum grade of GVHD, and were associated with a greater risk of death relative to other known risk factors (hazard ratio, 1.78). We conclude that elafin has significant diagnostic and prognostic value as a biomarker of skin GVHD.
Lightweight Aggregate Optimizes the Sustainability of Concrete, Through Weight Reduction, Internal Curing, Extended Service Life, and Lower Carbon Footprint. (2010)

This paper gives an overview on how lightweight aggregate (LWA) plays an important role in today's move toward sustainable concrete. It covers how lightweight aggregate provides value in sustainable construction and includes new insights and practices that can extend the service life of concrete pavements and bridges. Even though most lightweight aggregates require energy to manufacture, when used and evaluated from a whole building perspective the overall carbon footprint of a structure is often lowered. The use of lightweight aggregate provides strategies to enhance cement hydration, improve the energy performance of structures, reduce life-cycle costs and reduce environmental impacts of material transport and construction. The latest information on internal curing, suspended floor drying rates, roofs and how the use of lightweight aggregate contributes to LEED™ certification are addressed. Introduction. The benefits that have made using lightweight aggregate (LWA) economical for nearly 100 years are the same characteristics that make the material what is now being called The use of LWA helps designers, contractors and owners optimize the design, construction and long-term performance of concrete structures. By definition, sustainability must be looked at with a long-term perspective. A product that is considered green today but lowers the durability and long-term performance of structures is not sustainable. Conversely, when the energy used to produce a product, in this case LWA, is quickly recovered both in first and ongoing life cycle costs, the material is undoubtedly sustainable. LWA has been contributing to the sustainability of the site and structure of building projects long before the current movement came to the forefront. Lightweight aggregate stands on a 2000 year old Foundation of Sustainability. Roman period: The first known use of lightweight concrete occurred more than 2000 years ago. There are several lightweight concrete structures in the Mediterranean region, but the three most notable structures were built during the early Roman Empire and include the Port of Cosa, the Pantheon Dome, and the Coliseum. The Port of Cosa, built about 273 B.C., used lightweight concrete made from natural volcanic materials. These early builders learned that expanded aggregates
Clinical Utility of Determination of HER-2/neu and EGFR Fragments in Serum of Patients with Metastatic Breast Cancer (2006)

Determination of HER-2/neu ECD levels in the serum measured by ELISA at the onset of metastatic disease could offer an alternative to immunohistochemistry of the primary tumor since serum levels are correlated with protein expression in primary tumors. In contrast, no such correlation was observed for EGFR.
Critical Current Density of Y–Ba–Cu Oxide Wires (1987)

We have prepared Y–Ba–Cu oxide superconducting wires with stainless-steel, Cu–Ni alloy or silver sheath by a powder metallurgy technique, and confirmed superconductivity in stainless-steel or silver-sheathed wires. During the hea-treatment, a dielectric layer was formed at the interface between the sheath and Y–Ba–Cu oxide in stainless-steel-sheathed and Cu–Ni alloy-sheathed wires. The diffusion of the sheath element into Y–Ba–Cu oxide was analyzed using EPMA. The highest critical current dernsity, 560 A/cm 2 , was obtained for a silver-sheathed wire which was heat-treated in oxygen flow. A multicored wire was produced.
Detection of<i>Neisseria Gonorrhoeae</i>by Dot-Enzyme Immunoassay Using Monoclonal Antibodies (1989)

A highly sensitive and specific dot-enzyme immunoassay for the detection of Neisseria gonorrhoeae was developed using a pool of monoclonal antibodies (MAbs). The MAbs were obtained following immunization of mice with lithium acetate extracted outer membrane (OM) preparations. Western immunoblotting experiments demonstrated that MAbs NG26 and NG38, both IgG2a, reacted with lipopolysaccharides (LPS) and with the major OM protein, P1, respectively, MAb NG28, an IgG3, did not react in Western immunoblotting, MAbs NG28 and NG38 failed to react with OM treated with proteolytic enzymes or with semi-purified preparation of LPS. MAb NG26 reacted with the same LPS preparation. Binding radioimmunoassay with live bacteria showed that all the MAbs adsorbed to cell surface-exposed antigenic determinants. The limit of detection of the dot-enzyme immunoassay was between 1 and 4 x 10(4) cfu per dot. Using a panel of 177 strains of N. gonorrhoeae, MAbs NG28 and NG38 recognized only P1A and P1B strains respectively. MAb NG26 reacted with P1A, P1B and non-typable strains. These MAbs did not react with other Neisseria species or other bacterial species. Using this pool, the dot-enzyme immunoassay had a sensitivity of 93.2% and a specificity of 100%.
Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast (1993)

We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.
Identification and regulation of angiotensin II receptor subtypes on NG108-15 cells. (1991)

NG108-15 cells, a neurally derived clonal cell line, express various components of the renin-angiotensin system and thus serve as a model of the cellular action of angiotensin (Ang) II. NG108-15 cells contain a high-affinity binding site for Ang II, with a Kd of 1.1 nM and a Bmax of 6.5 fmol/mg protein. Ang peptides competed for 125I-Ang II binding with an order of potency of Ang II greater than Ang-(2-8) much greater than Ang-(1-7). The subtype 1 (or B)-selective Ang II receptor antagonist DuP 753 as well as [Sar1,Ile8]Ang II and [Sar1,Thr8]Ang II competed for Ang II binding with high affinity, whereas the subtype 2 (or A)-selective Ang receptor antagonist CGP 42112A was partially effective only at a 300-fold higher concentration. When NG108-15 cells were induced to differentiate by treatment with dibutyryl cyclic adenosine 3',5'-monophosphate, the density of Ang II receptors increased dramatically, with little change in affinity (1.1 versus 4.2 nM) or competition by Ang peptides. In marked contrast to undifferentiated cells, CGP 42112A became a potent competitor (IC50, 1 nM) for the majority (90-95%) of Ang II binding, whereas DuP 753 competed for only 5-10% of the binding sites. Ang II caused a dose-dependent mobilization of cytosolic Ca2+ in undifferentiated NG108-15 cells through activation of phospholipase C and the production of inositol 1,4,5-trisphosphate. In these cells, Ca2+ mobilization was blocked by either DuP 753 or the sarcosine Ang II analogues, whereas CGP 42112A was ineffective. Ang II also mobilized intracellular Ca2+ in differentiated NG108-15 cells.(ABSTRACT TRUNCATED AT 250 WORDS)
A Learning-Based Approach to Reduce JPEG Artifacts in Image Matting (2013)

Single image matting techniques assume high-quality input images. The vast majority of images on the web and in personal photo collections are encoded using JPEG compression. JPEG images exhibit quantization artifacts that adversely affect the performance of matting algorithms. To address this situation, we propose a learning-based post-processing method to improve the alpha mattes extracted from JPEG images. Our approach learns a set of sparse dictionaries from training examples that are used to transfer details from high-quality alpha mattes to alpha mattes corrupted by JPEG compression. Three different dictionaries are defined to accommodate different object structure (long hair, short hair, and sharp boundaries). A back-projection criteria combined within an MRF framework is used to automatically select the best dictionary to apply on the object's local boundary. We demonstrate that our method can produces superior results over existing state-of-the-art matting algorithms on a variety of inputs and compression levels.