gebhart/patrepeval huggingface dataset:
Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells (1996)

The localization of the adenovirus type 5 34-kDa E4 and 55-kDa E1B proteins was determined in the absence of other adenovirus proteins. When expressed by transfection in human, monkey, hamster, rat, and mouse cell lines, the E1B protein was predominantly cytoplasmic and typically was excluded from the nucleus. When expressed by transfection, the E4 protein accumulated in the nucleus. Strikingly, when coexpressed by transfection in human, monkey, or baby hamster kidney cells, the E1B protein colocalized in the nucleus with the E4 protein. A complex of the E4 and E1B proteins was identified by coimmunoprecipitation in transfected HeLa cells. By contrast to the interaction observed in primate and baby hamster kidney cells, the E4 protein failed to direct the E1B protein to the nucleus in rat and mouse cell lines as well as CHO and V79 hamster cell lines. This failure of the E4 protein to direct the nuclear localization of the E1B protein in REF-52 rat cells was overcome by fusion with HeLa cells. Within 4 h of heterokaryon formation and with protein synthesis inhibited, a portion of the E4 protein present in the REF-52 nuclei migrated to the HeLa nuclei. Simultaneously, the previously cytoplasmic E1B protein colocalized with the E4 protein in both human and rat cell nuclei. These results suggest that a primate cell-specific factor mediates the functional interaction of the E1B and E4 proteins of adenovirus.
Elastic and Inelastic Scattering of High-Energy Electrons by Some (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mn>2</mml:mn><mml:mi>p</mml:mi></mml:math>)-Shell Nuclei on the Intermediate-Coupling Model (1957)

The M\o{}ller potential has been used to describe the electron-nucleon interaction and a formula has been worked out in the Born approximation for the differential cross sections of elastic and inelastic scattering of high-energy electrons by $2p$-shell nuclei on the intermediate-coupling model with neglect of configuration mixing. Preliminary calculations have been made for ${\mathrm{Be}}^{9}$ and ${\mathrm{C}}^{12}$ to test some of the qualitative features of the formula deduced, especially the $\ensuremath{\theta}$ dependence of the $\ensuremath{\sigma}(\ensuremath{\theta})$ vs $\ensuremath{\theta}$ curve for inelastic scattering with change of total angular momentum $J$.
Patients′ Attitudes and Prior Treatments in Neuropathic Pain: A Pilot Study (2002)

New, and even conventional, therapies are often not pursued, despite inadequate pain control. Several issues are discussed, including patient barriers to seeking pain management, patient and physician barriers to analgesic drug therapy, and appropriate use of and access to multidisciplinary pain centres. Failure to implement therapeutic advances in pain management not only hinders improvement in patient care, but also may render futile decades of research. Widespread professional, patient and public education, as well as continued interdisciplinary research on treatment barriers, is essential.
Cryotherapy in Sport Injury Management (1995)

Based on Ken Knight's book, Cryotherapy in Sport Injury Management (ISBN 0 87322 771 9), these two videos are designed for use by sports medici ne professionals to: help athletes understand cryotherapy and comply to this recommended self-therapy; teach sports first aid or athletic training courses; and make presentations to local sports groups. The first tape explains why cryotherapy works through animated depictions of the injury process at the cellular level and how cryotherapy slows this process. The tape then introduces the RICES approach - Rest, Icing, Compression, Elevation, and Stabilization - for immediate treatment of injuries. The second tape shows how to get back into the game quickly by using the advanced techniques of cryokinetics and cryostretch.
CD19 is functionally and physically associated with surface immunoglobulin. (1989)

The pan-B and B cell-specific sIg and CD19 antigens are functionally and physically associated in the presence of anti-Ig mAb. Incubation of B cells with anti-Ig antibodies causes rapid, specific, reversible, concentration-dependent, and unidirectional comodulation of CD19 on every mature B cell studied. Comodulation is produced by mAbs specific for the gamma, mu, kappa, and lambda chains of Ig, and by at least one idiotype-specific mAb. Comodulation is observed using 15 CD19-specific mAbs that detect at least three different CD19 epitopes. Of 18 surface antigens studied, only CD19 is comodulated. Loss of sIg and CD19 occurs concurrently during anti-Ig modulation and demonstrates a comparable dependence on anti-Ig concentration, suggesting that these are parallel rather than serial events. Incubation with anti-Ig specifically cocaps and suggests internalization of anti-CD19 mAb. Comodulation of sIg and CD19 by anti-Ig but not anti-CD19 mAbs suggests that ligand binding enables sIg to then interact with CD19. We propose that CD19 is a component of the B cell antigen receptor and suggest that it could facilitate signal transduction by sIg-antigen complexes.
Post-processing GNSS/INS Measurements Using a Tightly Coupled Fixed-Interval Smoother Performing Carrier Phase Ambiguity Resolution (2006)

This paper describes a high accurate tightly cou- pled, differentially corrected GNSS/INS system for survey appli- cations in post-processing mode. A Kalman filter is described, that calculates the navigation solution as well as the the GNSS carrier phase integer ambiguities. The LAMBDA method is used to resolve the integer ambiguities. To achieve an optimum solution based on all measurement data available, a Rauch-Tung- Striebel smoother is used. The combination of tight integration and smoothing results in a system which is capable to resolve almost all of the carrier phase ambiguities and therefore provides centimeter accuracy, even if satellites are only available for short periods. The smoother provides steady results without discontinuities and bridges GNSS outages well. Hence, the system overcomes problems which are typical for kinematic ground surveys.
Inhibitor(s) of prostaglandin biosynthesis in extracts of oat (<i>Avena sativa</i>) seeds (1981)

Conference Article| October 01 1981 Inhibitor(s) of prostaglandin biosynthesis in extracts of oat (Avena sativa) seeds SHEIKH A. SAEED; SHEIKH A. SAEED 1Research Department, Miles Laboratories Limited, Stoke Poges, Slough, SL2 4LY, Berks, U.K. Search for other works by this author on: This Site PubMed Google Scholar NAEEM M. BUTT; NAEEM M. BUTT 2Research Department, Miles Laboratories Limited, Stoke Poges, Slough, SL2 4LY, Berks, U.K. Search for other works by this author on: This Site PubMed Google Scholar WENDY J. McDONALD-GIBSON; WENDY J. McDONALD-GIBSON 3Research Department, Miles Laboratories Limited, Stoke Poges, Slough, SL2 4LY, Berks, U.K. Search for other works by this author on: This Site PubMed Google Scholar HARRY O. J. COLLIER HARRY O. J. COLLIER 4Research Department, Miles Laboratories Limited, Stoke Poges, Slough, SL2 4LY, Berks, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1981) 9 (5): 444. https://doi.org/10.1042/bst0090444 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation SHEIKH A. SAEED, NAEEM M. BUTT, WENDY J. McDONALD-GIBSON, HARRY O. J. COLLIER; Inhibitor(s) of prostaglandin biosynthesis in extracts of oat (Avena sativa) seeds. Biochem Soc Trans 1 October 1981; 9 (5): 444. doi: https://doi.org/10.1042/bst0090444 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1981 Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.
Pharmacogenomics and therapeutic prospects in Alzheimer’s disease (2005)

Approximately 10-20% of the direct costs of Alzheimer's disease are attributed to pharmacological treatment. Less than 20% of Alzheimer's disease patients are moderate responders to conventional drugs (e.g., donepezil, rivastigmine, galantamine, memantine) with doubtful cost-effectiveness. In total, 15% of the Caucasian population with Alzheimer's disease are carriers of defective CYP2D6 polymorphic variants that are potentially responsible for therapeutic failures when receiving cholinesterase inhibitors and psychotropic drugs. In addition, structural genomics studies demonstrate that > 100 genes might be involved in Alzheimer's disease pathogenesis, regulating dysfunctional genetic networks leading to premature neuronal death. The Alzheimer's disease population exhibits a higher genetic variation rate than the control population, with absolute and relative genetic variations of 40-60% and 0.85-1.89%, respectively. Alzheimer's disease patients also differ from patients with other forms of dementia in their genomic architecture, possibly with different genes acting synergistically to influence the phenotypic expression of biological traits. Functional genomics studies in Alzheimer's disease reveal that age of onset, brain atrophy, cerebrovascular haemodynamics, brain bioelectrical activity, cognitive decline, apoptosis, immune function and amyloid deposition are associated with Alzheimer's disease-related genes. Pioneering pharmacogenomics studies also demonstrate that the therapeutic response in Alzheimer's disease is genotype-specific, with APOE-4/4 carriers as the worst responders to conventional treatments. It is likely that pharmacogenetic and pharmacogenomic factors account for 60-90% of drug variability in drug disposition and pharmacodynamics. The incorporation of pharmacogenomic/pharmacogenetic protocols in Alzheimer's disease may foster therapeutic optimisation by helping to develop cost-effective drugs, improving efficacy and safety, and reducing adverse events and cutting-down unnecessary cost for the industry and the community.
Simultaneous acquisition of cerebral blood volume‐, blood flow‐, and blood oxygenation‐weighted <scp>MRI</scp> signals at ultra‐high magnetic field (2014)

Purpose Yang et al. proposed an MRI technique for the simultaneous acquisition of cerebral blood volume (CBV), cerebral blood flow (CBF), and blood oxygenation level‐dependent (BOLD)‐weighted MRI signals (9). The purpose of this study was to develop modified version of the Yang sequence, which utilizes the advantages of 7 Tesla, leading to a robust and reliable MRI sequence. Methods The inversion recovery‐based MR pulse sequence introduced here involves slice‐saturation slab‐inversion vascular space occupancy (SI‐SS‐VASO) MRI, double echo planar imaging readouts for arterial spin labeling, and VASO in order to correct for BOLD contamination, and a separate BOLD acquisition to minimize inversion effects on the BOLD signal. A standard visual stimulus block design was used to evaluate the spatial and temporal characteristics of CBV‐, CBF‐, and BOLD‐weighted images. Results The high signal‐to‐noise ratio and spatial resolution of this method leads to robust activation maps. This technique enables the investigation of the differential spatial specificity and temporal characteristics of the different modalities. Conclusion The pulse sequence could be a powerful tool for studies of neurovascular coupling, hemodynamic response, or calibrated BOLD. Magn Reson Med 74:513–517, 2015. © 2014 Wiley Periodicals, Inc.
Enhanced immune response with a combination of alum and biodegradable nanoparticles containing tetanus toxoid (2001)

The purpose of the study was to determine the synergistic adjuvant effect of sustained release biodegradable nanoparticles in combination with alum. Nanoparticles containing tetanus toxoid (TT) were formulated using a biodegradable polymer, polylactic polyglycolic acid co-polymer (50:50, molecular weight 100000). The immunization studies were carried out subcutaneously in rats. The results were expressed as mean serum anti-TT IgG levels. I'he nanoparticles demonstrated a TT loading of 4% w/w with mean particle diameter of 238 +/- 31 nm. The TT encapsulated in nanoparticles was released slowly under in vitro conditions, with 67.5% cumulative release occurring in 20 days. A single injection of TT-nanoparticles (TT dose = 10 Lf) mixed with TT-Alum (TT dose= 5 Lf) induced a four-fold greater mean serum anti-TT IgG response than a single injection of TT nanoparticles alone (TT dose=15Lf) (2235 +/- 310 vs. 539 +/- 49 microg/ml, mean +/- sem, p = >0.001). In addition, the mean immune response induced with the single injection of combination of nanoparticles and alum was comparable to the two injections of TT-alum alone (5 Lf each dose) given at 3 week intervals IgG = 1998 +/- 333 microg/ ml). Furthermore, the combination induced a peak immune response (IgG = 4215 +/- 546 microg/ml) as early as the first time point at 3 weeks post-immunization. In the case of a TT-alum alone, the animals showed a weaker immune response at 3 weeks and required a second dose of TT-alum to enhance the antibody response. The data thus suggest that the combination of TT-nanoparticles and TT-alum acts as a much better adjuvant than nanoparticles or alum alone. A rapid induction of immune response is useful to curb the spread of communicable diseases in the case of an outbreak.
A simple synthesis of mesoporous carbons with tunable mesopores using a colloidal template-mediated vapor deposition polymerization (2005)

Mesoporous carbons with highly uniform and tunable mesopores were fabricated by one-step vapor deposition polymerization (VDP) using colloidal silica particles as templates and polyacrylonitrile (PAN) as a carbon precursor.
572. Pyrazine derivatives. Part XI. Synthesis of cyclic hydroxamic acids related to aspergillic acid (1949)

G. Dunn, J. A. Elvidge, G. T. Newbold, D. W. C. Ramsay, F. S. Spring and W. Sweeny, J. Chem. Soc., 1949, 2707 DOI: 10.1039/JR9490002707
[Comparative effects of transurethral incision (TUIP) and the combination of TUIP and LHRH agonists in the treatment of benign prostatic hypertrophy]. (1996)

Between December 1991 and December 1993, 74 BPH patients with an increased operative risk and concomitant diseases such as diabetes mellitus and hypertension were submitted to a transurethral incision of the prostate (TUIP). After TUIP, patients were randomized to two different groups: group 1 was followed without additional treatment and group 2 received an LHRH analogue for the first 6 months of follow-up. With respect to transurethral resection of the prostate (TURP), TUIP has been shown to demonstrate a lower perioperative morbidity. This advantage has lent further support to this technique as a valid alternative for patients in poor general conditions who are at high risk with more invasive procedures. One of the limits of TUIP is the long-term effectiveness. Aim of this study was to ascertain whether in patients with BPH and an increased operative risk who require immediate and definitive treatment but with a low perioperative morbidity, the long-term effectiveness of TUIP can be stabilized by the administration of an LHRH analogue. At present postoperative follow-up ranges from a minimum of 24 months to a maximum 48 months (mean 38.4 months). Perioperative morbidity rate associated with TUIP was 8.1%. In the group randomized to combination therapy (TUIP + LHRH analogue), the clinical condition of the patients was not modified by LHRH analogue treatment and none of the patients withdrew from treatment. Loss of sexual potency occurred in all patients on LHRH analogue, however, none of these patients discontinued treatment for this reason. At the end of the cycle of hormone treatment, sexual potency returned to pretreatment values in 69.5% of patients after a mean of 3.2 months. In this study the objective efficacy of the treatment was evaluated using flow rate measurements, and the subjective assessment of outcomes, using the International Prostate Symptom Score. Statistically significant differences between the two groups (TUIP alone or TUIP + LHRH analogue) (p < 0.01) were reported at 6 months and were still maintained at 24 months of follow up. Results emerging from this investigation confirm that TUIP may be considered extremely safe procedure with low operative risk. In selected BPH patients who are at high risk, with a more invasive procedure and who must be submitted to immediate and definitive treatment, the association of an LHRH analogue seems to increase the long-term effectiveness of TUIP. Five year follow-up studies are still in progress.
Comparison Between Dynamic Contour Tonometry and Goldmann Applanation Tonometry (2009)

IOP measured by DCT was not affected by CCT in eyes with low to normal CCT, whereas this measurement was affected in eyes of high CCT range. CCT may have less effect on IOP measurements using DCT than those obtained by GAT, within a specified range of CCT.
Morphologic features of chronic hepatitis associated with primary sclerosing cholangitis and chronic ulcerative colitis (1981)

Histologic, ultrastructural, chemical, and statistical methods were used to study liver biopsy and autopsy specimens from 43 patients who had primary sclerosing cholangitis (PSC), with or without chronic ulcerative colitis (CUC), and from 19 patients who had CUC without PSC. In all study groups, essentially the same abnormalities were found in the hepatic parenchyma outside the major bile ducts, although nondiagnostic tissue samples were observed also. Specimens from patients with extrahepatic PSC were indistinguishable from those patients with combined extra- and intrahepatic PSC. Common findings included periductal fibrosis and inflammation, portal edema and fibrosis, focal proliferation of bile ducts and ductules, focal bile duct obliteration and loss of bile ducts, copper deposition, and cholestasis. Proliferation of bile ducts in some portal tracts and obliteration or absence of bile duct in others were the most characteristic changes. In most specimens, inflammatory changes appeared mild, yet biliary cirrhosis had developed in 34% of the patients. Specimens from patients with PSC, with or without CUC, more often contained bile and strikingly increased stainable copper (Grades 2 and 3) than did specimens from patients with CUC without PSC. Hepatic copper contents, measured by atomic absorption spectrophotometry, also were higher in specimens from patients with PSC. Study of PCS specimens by transmission electron microscopy and by energy-dispersive X-ray microanalysis revealed that most copper was sequestered in lipolysosomes. The recognition of strikingly similar morphologic features in many liver specimens from patients with either PSC or CUC or both suggests that the causes of these conditions are closely related.
RICE BREEDING AND INHERITANCE OF HERBICIDE RESISTANCE IN CLEARFIELD RICE ( Oryza sativa L.) (2007)

An ethyl methane sulfonate-induced mutation of the acetolactate synthase. The first two herbicide-resistant rice varieties available for commercial production in southern United States were the imidazolinone-resistant varieties 'CL 121' and 'CL 141' by Clear Field. Six modern varieties as OM2717, AS996, OM2395, OMCS2000, IR64, OM1490 from CLRRI and seven lines from Clear Field Company (B1, B2, B3, B4, B5, B6, B7 carrying herbicide resistance gene) were crossed in all possible combinations without reciprocals. These crosses segregated into 3:1 resistant to susceptible, suggesting that a dominant gene controls resistance to herbicide. This was confirmed by reaction of F 3 progenies, which segregated into 1:2:1 homozygous resistant to segregating to homozygous susceptible. Polymorphic marker RM267, RM262, RM285, RM256, RM271, RM44, RM261 detected target gene in the progenies. Five lines namely OM5712, OM5727, OM5728, OM5749, OM5755 exhibited survival ability when herbicide applied. Forty-three lines have reduced to survive un nder stress (40-95% died). OMCF9 (OM 2395 / B2) and OMCF6 (IR64 / B1) obtained the highest yield. They exhibited their tolerance to imazapyr herbicide.
ICAM-1 and VCAM-1 mediate endotoxemic myocardial dysfunction independent of neutrophil accumulation (2002)

Both intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) have been implicated in neutrophil-mediated lung and liver injury during sepsis. However, the role of these adhesion molecules as well as the contribution of neutrophils in myocardial dysfunction during sepsis remains to be determined. The purpose of this study was to examine the role of ICAM-1, VCAM-1, and neutrophils in lipopolysaccharide (LPS)-induced myocardial dysfunction. Mice were subjected to LPS (0.5 mg/kg ip) or vehicle (normal saline), and left ventricular developed pressure (LVDP) was determined by the Langendorff technique. LVDP was depressed by nearly 40% at 6 h after LPS. Immunofluorescent staining revealed a temporal increase in myocardial ICAM-1/VCAM-1 expression and neutrophils after LPS. Antibody blockade of VCAM-1 reduced myocardial neutrophil accumulation and abrogated LPS-induced cardiac dysfunction. Antibody blockade or absence of ICAM-1 (gene knockout) also abrogated LPS-induced cardiac dysfunction but did not reduce neutrophil accumulation. Neutrophil depletion (vinblastine or antibody) did not protect from LPS-induced myocardial dysfunction. Our results suggest that although endotoxemic myocardial dysfunction requires both ICAM-1 and VCAM-1, it occurs independent of neutrophil accumulation.
Genotyping and mRNA profiling reveal actionable molecular targets in biliary tract cancers. (2018)

Biliary tract cancer (BTC) represents a heterogeneous disease with dismal outcome. Herein, we examined genotype and angiogenesis features in BTC. We applied genotyping (Sanger, qPCR, 101-gene panel NGS), mRNA relative quantification methods, and β-catenin immunohistochemistry in 84 FFPE BTC (55 gallbladder [GBC], 14 intrahepatic [ICC], 15 extrahepatic [ECC] carcinomas). We identified 541 mutations in 68 (81%) tumors. Top mutated genes were CTNNB1 (36%); PTEN (33%); TP53 (31%); PIK3R1 (29%); PIK3CA (13%); BRCA2 and KRAS (12%); BRCA1 (11%). Six GBCs were hypermutated [hm] displaying a distinct mutational pattern. Mutations in TP53 and PI3K, Wnt and RAS components were prevalent among non-hypermutated tumors. All hmGBCs carried mutations in BRCA2 and other homologous recombination repair (HRR) genes, in PD1, but not in CTNNB1 and KRAS. None of the pathogenic BRCA2 p.D2723G and BRCA1 p.Q563* and c.5266dupC was present at frequencies expected for germline mutations. We observed copy gains (>6 copies) in EGFR (9% of informative tumors), PRKAR1A (7%), PIK3CA (6%), ERBB2 (5%) and MET (4%). TP53 mutations were prevalent in GBC (P<0.001) and PRKAR1A copy gains in ICC (P=0.007). PTEN was frequently co-mutated with CTNNB1 (P<0.001). Unrelated to CTNNB1 mutations, nuclear β-catenin was detected in 45% of tumors, among them in 5/6 hmGBC. We observed strong mRNA expression correlation of the two neuropilins (NRP1 and NRP2) with each other (Spearman's rho 0.59) and with the endothelin receptor (NRP2 rho 0.66; NRP2 rho 0.51), and between VEGFA and its receptors (FLT1 rho 0.49; KDR rho 0.45). All PIK3CA mutated tumors expressed endothelin 1 mRNA (P=0.010). Most tumors expressing nuclear β-catenin were negative for VEGFC (P=0.009) and FLT4 (P=0.002) mRNA expression. In conclusion, we confirmed the presence of known genomic aberrations in BTC and different genotypes between BTC subsets. Novel findings are the coexistence of PI3K and WNT pathway gene alterations in BTC, their association with angiogenesis, and the hypermutated GBCs with HRR gene mutations, all of which may be considered for new treatment options in this difficult to treat disease.