gebhart/patrepeval huggingface dataset:
Der3p/Hrd1p Is Required for Endoplasmic Reticulum-associated Degradation of Misfolded Lumenal and Integral Membrane Proteins (1998)

We have studied components of the endoplasmic reticulum (ER) proofreading and degradation system in the yeast Saccharomyces cerevisiae. Using a der3-1 mutant defective in the degradation of a mutated lumenal protein, carboxypeptidase yscY (CPY*), a gene was cloned which encodes a 64-kDa protein of the ER membrane. Der3p was found to be identical with Hrd1p, a protein identified to be necessary for degradation of HMG-CoA reductase. Der3p contains five putative transmembrane domains and a long hydrophilic C-terminal tail containing a RING-H2 finger domain which is oriented to the ER lumen. Deletion of DER3 leads to an accumulation of CPY* inside the ER due to a complete block of its degradation. In addition, a DER3 null mutant allele suppresses the temperature-dependent growth phenotype of a mutant carrying the sec61-2 allele. This is accompanied by the stabilization of the Sec61-2 mutant protein. In contrast, overproduction of Der3p is lethal in a sec61-2 strain at the permissive temperature of 25 degrees C. A mutant Der3p lacking 114 amino acids of the lumenal tail including the RING-H2 finger domain is unable to mediate degradation of CPY* and Sec61-2p. We propose that Der3p acts prior to retrograde transport of ER membrane and lumenal proteins to the cytoplasm where they are subject to degradation via the ubiquitin-proteasome system. Interestingly, in ubc6-ubc7 double mutants, CPY* accumulates in the ER, indicating the necessity of an intact cytoplasmic proteolysis machinery for retrograde transport of CPY*. Der3p might serve as a component programming the translocon for retrograde transport of ER proteins, or it might be involved in recognition through its lumenal RING-H2 motif of proteins of the ER that are destined for degradation.
The <i>SERPINE2</i> Gene is Associated with Chronic Obstructive Pulmonary Disease in Two Large Populations (2007)

These data provide further support for SERPINE2 as a COPD susceptibility gene.
A high‐molecular‐weight outer membrane protein of <i>Xanthomonas oryzae</i> pv. <i>oryzae</i> exhibits similarity to non‐fimbrial adhesins of animal pathogenic bacteria and is required for optimum virulence (2002)

Transposon insertions in a novel 3.798 kb open reading frame (ORF) of the rice pathogen, Xanthomonas oryzae pv. oryzae (Xoo) cause virulence deficiency and altered colony/lawn morphology. This ORF encodes a protein, XadA, of 1,265 amino acids that exhibits significant similarity to non-fimbrial adhesins of animal pathogenic bacteria such as Yersinia YadA and Moraxella UspA1. An interesting feature is that the YadA similarity region is repeated six times within the XadA sequence and encompasses almost the entire length of the protein. Anti-XadA antibodies identified a 110 kDa outer membrane protein that was sensitive to protease treatment of whole cells. XadA expression is induced in minimal medium. Homology modelling suggests that XadA adopts a beta-helix conformation-like pertactin, a non-fimbrial adhesin of Bordetella pertussis. This work is the first characterization of a non-fimbrial adhesin-like molecule in a plant pathogenic bacterium. It extends our knowledge about the repertoire of homologous virulence factors that are deployed by animal and plant pathogenic bacteria to include functions potentially involved in adhesion.
MA57---a code for the solution of sparse symmetric definite and indefinite systems (2004)

We introduce a new code for the direct solution of sparse symmetric linear equations that solves indefinite systems with 2 × 2 pivoting for stability. This code, called MA57, is in HSL 2002 and supersedes the well used HSL code MA27. We describe some of the implementation details and emphasize the novel features of MA57. These include restart facilities, matrix modification, partial solution for matrix factors, solution of multiple right-hand sides, and iterative refinement and error analysis. The code is written in Fortran 77, but there are additional facilities within a Fortran 90 implementation that include the ability to identify and change pivots. Several of these facilities have been developed particularly to support optimization applications, and we illustrate the performance of the code on problems arising therefrom.
모자보호시설에 거주하는 이혼여성 한부모가족의 가족탄력성이 가족적응에 미치는 영향 (2007)

본 연구에서는 모자보호시설에 입소한 이혼여성 한부모가족을 대상으로 가족탄력성이 가족적응에 어떠한 영향을 미치는가를 살펴보았다. 이를 위해 전국 40개의 모자보호시설 중 연구를 허락한 10개의 시설에 우편으로 설문지를 전달하였고 회수된 114부의 자료를 분석에 사용하였다. 주요 연구결과를 요약하면 다음과 같다. 첫째,...
Screen Printed Thin Film CdS/CdTe Solar Cell (1980)

Thin film CdS/CdTe solar cells with an efficiency of 6.3 % have been prepared on a borosilicate glass substrate of 4s×4 cm2 by successively repeating screen printing and heating (sintering) of each paste of CdS, CdTe and C. The CdS paste consists of CdS, CdCl2, GaCl2 and propylene glycol (PG). The CdTe paste contains CdCl2 and PG, and the C paste contains PG and a small amount of acceptor impurity. During the heating of C paste, an n·CdS/p·CdTe heterojunction is formed. In the most efficient cell, the peak of electron voltaic effect exists within 1 µm of the CdTe side from the CdS/CdTe metallurgical boundary. The C electrode cell is more stable than the previous Cu2Te electrode cell for an accelerated life test. From 25 elemental cells with 4×4 cm2 substrate, a 1 watt module has tentatively been constructed with a module efficiency of 2.9 %.
Selective use of image-guided large-core needle biopsy of the breast: accuracy and cost-effectiveness. (1995)

Large-core needle biopsy is an accurate and cost-effective method for sampling breast lesions when used in a selective fashion.
Plasma carnitine insufficiency and effectiveness of <scp>L</scp>‐carnitine therapy in patients with mitochondril myopathy (1993)

Plasma carnitine "insufficiency," (plasma esterified carnitine to free carnitine ratio above 0.25) was found in 21 of 48 (43.8%) patients with mitochondrial myopathy, of whom 4 also showed both total and free carnitine deficiencies in plasma. In addition, plasma levels of SCAC and LCAC were higher in patients with mitochondrial myopathy than in controls (P < 0.001 and P < 0.01, respectively). Patients diagnosed as having plasma carnitine insufficiency or deficiency were treated with L-carnitine (50-200 mg/kg per day in four daily doses). Muscle weakness improved in 19 of 20 patients, failure to thrive in 4 of 8, encephalopathy in 1 of 9, and cardiomyopathy in 8 of 8 patients. Plasma carnitine "insufficiency" provides an additional clue to the diagnosis of mitochondrial myopathy and an indication for L-carnitine therapy.
Multiple Column Synthesis of a Library of T‐Cell Stimulating T<sub>n</sub>‐Antigenic Glycopeptide Analogues for the Molecular Characterization of T‐Cell–Glycan Specificity (1996)

A series of peptides and glycopeptides derived by amino acid and glycosyl amino acid scans through the self peptide from CBA/J mouse haemoglobin Hb (67-76). VITAFNEGLK, was synthesized by multiple column peptide synthesis (MCPS). Investigation of glycopeptide binding to the mouse major histocompatibility class II molecule Ek showed that glycans in position 72 did not interfere with the binding to Ek. Immunization experiments revealed that glycopeptides with the glycan in position 72 were immunogenic. Therefore a series of N-linked and O-linked glycopeptides with the glycan attached in the position 72 either to serine, threonine or asparagine was synthesized by MCPS. The glycan structure was furthermore varied with respect to monosaccharide component, size of oligosaccharide, anomer configuration and stereochemistry of essential hydroxyl groups in order to investigate the specificity of the interaction with the T-cell receptor. Easy synthesis of ready to use Ser and Thr building blocks corresponding to mucin core 1, the Tn-antigen and its beta-anomer were developed using trichloroacetimidates as glycosyl donors and reduction with in situ acetylation of the azide containing glycosylation products. Synthesis of an alpha-linked GlcNAc-Thr building block was achieved by glycosylation of Fmoc-Thr-OPip with 2-azido-2-deoxy-3,4,6-tri-O-acetyl-D-glycopyranosyl trichloroacetimidate as a glycosyl donor. Other building blocks were obtained by previously described procedures.
Neonatal alloimmune thrombocytopenic purpura and congenital porencephaly in two siblings associated with a "new" maternal antiplatelet antibody (1985)

We report a brother and sister, both of whom have porencephaly, hydrocephalus, optic atrophy, severe mental retardation, and spastic quadriplegia. In the younger child, abnormal intracranial structure was demonstrated by sonography at 32 weeks' gestation and was suspected earlier. Both children had transient severe thrombocytopenia as newborns. The mother is healthy and has never had purpura or other bleeding symptoms. However, her serum was found to react strongly with platelets from the father and from both children. The antibody in the mother's serum is platelet-specific but does not appear to be directed against any of the known antigens associated with neonatal alloimmune thrombocytopenic purpura (NATP) in other families, including PlA1, PlE2, or Baka. Although the mother's serum reacts with platelets from all 47 unrelated normal donors tested and from both the mothers and the fathers of 17 other children with suspected NATP, it does not react with her own platelets or with platelets from a patient with Glanzmann's thrombasthenia. These observations suggest that the serum from this woman identifies a previously undescribed high-frequency platelet-specific alloantigen and that sensitization to this determinant caused severe immune thrombocytopenia in both her children. It is likely that this led to intracranial hemorrhage in utero in these cases.
Single-shot THz Spectrometer for Measurement of RF Breakdown in mm-wave Accelerators (2016)

We present a new instrument designed to detect RF pulse shortening caused by vacuum RF breakdown in mm-wave particle accelerators. RF breakdown limits the performance of high gradient RF accelerators. To understand the properties of these breakdowns, it is necessary to have diagnostics that reliably detect RF breakdowns. In X-band or S-band accelerators, RF breakdowns are detected by measuring RF pulse shortening, vacuum burst, or, if current monitors are available, spikes in the field-emitted currents. In mm-wave accelerators, all of these methods are difficult to use. In our experiments, we could not measure RF pulse shortening directly with a crystal detector because the RF pulse is very short'just a few nanoseconds'and changes in the measured signal were masked by RF amplitude jitter. To overcome this limitation, we built a single-shot spectrometer with a frequency range of 117-125 GHz and a resolution of 0.1 GHz. The spectrometer should be able to measure the widening of the spectrum caused by the shortening of nanosecond-long pulses. We present design considerations, first experimental results obtained at FACET, and planned future improvements for the spectrometer.
Supercritical Fluid Extraction of Natural Antioxidants from Rosemary:  Comparison with Liquid Solvent Sonication (1997)

Supercritical fluid extraction (SFE) and liquid solvent sonication, in combination with two different sample treatments, were compared for the extraction of natural antioxidants from rosemary leaves. Dried, ground, and sieved rosemary leaves (20 mg) were subjected to SFE with CO(2) at 355 bar at 100 °C (CO(2) density 0.72 g/mL) for 20 min at a liquid flow rate of 4 mL/min. The analytes were concentrated on an ODS trap and subsequently eluted with acetone. Antioxidants in the SF and liquid solvent extract were analyzed by HPLC. Compounds of known antioxidant activity such as carnosol, carnosic acid, and methyl carnosate were identified by mass spectrometry of the HPLC fractions collected. Freezing and grinding the samples in liquid nitrogen resulted in decreased carnosic acid recoveries. Supercritical CO(2) extraction provided the highest recovery of carnosic acid from rosemary leaves (35.7 mg/g), the lowest relative standard deviation (4.4%), and the cleanest extract [Formula: see text] no cleanup prior to HPLC was required. Among the liquid solvents studies, only acetone provided comparable results (73% recovery relative to SC-CO(2) extraction); however, it required decoloration with active carbon prior to HPLC analysis.
Bioencapsulation and application of Lactobacillus plantarum isolated from catfish gut as an antimicrobial agent and additive in fish feed pellets (2014)

Lactobacillus plantarum was isolated from fresh water catfish collected from a local market. The probiotic-like characteristics of the bacteria, and its antibacterial effects against common food-borne and fish pathogens, were investigated by growing it in different diverse conditions (varying osmolarity, bile salt, acid and alkaline conditions, and temperature). The L. plantarum was encapsulated in a mixture of alginate and fish gelatin protein at different concentrations to enhance its protection during incorporation into fish feed pellets. The significance of encapsulation was proven by exposing the encapsulated L. plantarum to harsh conditions in comparison to un-encapsulated cells as a control. The results suggest that 2 % alginate and 13 % gelatin coating material had optimal textural properties, with a hardness of 1.436 N, high viscosity of 20.23 cP and highest encapsulation efficiency with 8.45 log colony forming units (CFU). The bacteria showed antagonistic effects against the fish pathogen Streptococcus agalactiae, and other common pathogens such as Salmonella typhimurium, Escherichia coli and Klebsiella pneumoniae. Encapsulated L. plantarum was found to tolerate pH 2 and pH 8 more efficiently compared to un-encapsulated cells, and encapsulated cells survived 6 log CFU from an initial 8.23 log CFU count at low pH 2 after a 90-min incubation and showed resistance to 50 °C for 1 h. Thus, the isolated L. plantarum was found to have potential to be used successfully in fish feed and other similar applications.
On-the-Fly Fluorescence Lifetime Detection in Liquid Chromatography with Data Collected Simultaneously at Multiple Emission Wavelengths (1999)

A novel liquid chromatography (LC) fluorescence detector simultaneously generates fluorescence decay curves at multiple emission wavelengths. The fourth harmonic (266 nm) excitation light from a pulsed Nd:YAG laser is focused into a standard LC flow cell. A small spectrograph disperses the fluorescence. Fiber-optic delay lines positioned in the exit focal plane time the arrival of photons at the photomultiplier tube detector. Four wavelength channels with 50 ns channel spacing were used in this study. The intensity-decay time waveform is averaged with a digital storage oscilloscope. Temporal overlap between adjacent wavelength channels is removed during the post-processing of the data. System performance was characterized for chromatographic elution of polycyclic aromatic hydrocarbons (PAHs). Detection limits are 1.6 ppb for naphthalene and 0.14 ppb for fluorene. Resolution of co-eluting 1,4-dimethyl-naphthalene and anthracene components on the basis of differences in their lifetimes and spectral distributions is demonstrated.
Impact of leflunomide versus biologic agents on the costs of care for rheumatoid arthritis in a managed care population. (2002)

Leflunomide is associated with reduced costs of medications and other healthcare services relative to biologic medications among managed care patients with RA.
The trade credit in the Spanish agrofood industry (2011)

Selling on credit is rather frequent in Mediterranean countries. Its generalized use can lead to excessive enlargements of the payment periods and consequently can deteriorate the profitability of firms. In spite of the relevance of this problem there are few empirical researches. This work intends to fill this gap and to shed light on the factors related to the extension of trade credit. In the theoretical and empirical literature, different motives have been proposed to explain this issue: a mechanism to reduce transaction costs, a financial alternative to the bank system and an additional tool to improve commercial activities. To contrast these ideas a panel of 388 firms of the Spanish agrofood industry has been taken, and static and dynamic regression models have been estimated by using robust methods to heteroskedasticity, autocorrelation and endogeneity of the explanatory variables. The results confirm that trade credit receivable is associated with more active firms and with cheaper bank financing. Other factors with positive relationships are short-term bank debts and accounts payable. These findings are consistent with commercial motives, rather than a pure financial view, in the sense that financial distressed producers extend trade credit as a way of promoting their products and in turn increasing their sales.
Effects of pyruvate dose on <i>in vivo</i> metabolism and quantification of hyperpolarized <sup>13</sup>C spectra (2011)

Real-time in vivo measurements of metabolites are performed by signal enhancement of [1-(13)C]pyruvate using dynamic nuclear polarization, rapid dissolution and intravenous injection, acquisition of free induction decay signals and subsequent quantification of spectra. The commonly injected dose of hyperpolarized pyruvate is larger than typical tracer doses, with measurement before complete dilution of the injected bolus. Pyruvate is in exchange with its downstream metabolites lactate, alanine and bicarbonate. A transient exposure to high pyruvate blood concentrations may cause the saturation of cellular uptake and metabolic conversion. The goal of this study was to examine the effects of a [1-(13)C]pyruvate bolus on metabolic conversion in vivo. Spectra were quantified by three different methods: frequency-domain fitting with LCModel, time-domain fitting with AMARES and simple linear least-squares fitting in the time domain. Since the simple linear least-squares approach showed bleeding artifacts and LCModel produced noisier time signals. AMARES performed best in the quantification of in vivo hyperpolarized pyruvate spectra. We examined pyruvate doses of 0.1-0.4 mmol/kg (body mass) in male Wistar rats by acquiring slice-selective free induction decay signals in slices dominated by heart, liver and kidney tissue. Dose effects were noted in all cases, except for alanine in the cardiac slice below the dose of 0.2 mmol/kg. Our results indicate unlimited cellular uptake of pyruvate up to this dose and limited enzymatic activity of lactate dehydrogenase. In the cardiac slice above 0.2 mmol/kg and in liver and kidney slices, reflect limited cellular uptake or enzymatic activity, or a combination of both effects. The results indicate that the dose of pyruvate must be recognized as an important determinant for metabolic tissue kinetics, and saturation effects must be taken into account for the quantitative interpretation of the observed results.
The Isolation of a Cyclic Intermediate in the Ketone—Alkoxyacetylene Reaction (1965)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Isolation of a Cyclic Intermediate in the Ketone—Alkoxyacetylene ReactionW. J. MiddletonCite this: J. Org. Chem. 1965, 30, 4, 1307Publication Date (Print):April 1, 1965Publication History Published online1 May 2002Published inissue 1 April 1965https://pubs.acs.org/doi/10.1021/jo01015a544https://doi.org/10.1021/jo01015a544research-articleACS PublicationsRequest reuse permissionsArticle Views240Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Harmonically Informed Multi-Pitch Tracking. (2009)

[TODO] Add abstract here.
C3d immunohistochemistry on formalin‐fixed tissue is a valuable tool in the diagnosis of bullous pemphigoid of the skin (2009)

C3d immunohistochemistry is a valuable tool in the diagnosis of BP and PG of the skin with a sensitivity of at least 97%. Mixed forms of linear IgA dermatosis, and BP, DHD and linear IgA dermatosis can only be identified by DIF. A positive result may prompt serologic confirmation of BP without further need for DIF.
Improving Bedding Plant Quality and Stress Resistance with Low Phosphorus (1998)

Bedding plants are frequently exposed to water stress during the postproduction period, resulting in reduced quality. We demonstrated that alumina-buffered P fertilizer (Al-P) provides adequate but much lower P concentrations than conventionally used in soilless mixes. When impatiens ( Impatiens wallerana Hook. f. `Impulse Orange') and marigold ( Tagetes patula L. `Janie Tangerine') plants were grown with reduced phosphorus using Al-P, P leaching was greatly reduced and plant quality was improved. Diameter of impatiens plants and leaf area of plants of both species were reduced by Al-P. Marigold plants grown with Al-P had more flowers and fewer wilted flowers. Flower wilting was also reduced for impatiens plants grown with Al-P. In marigold plants, roots were confined to a small volume beneath the drip tube in control plants, while roots of Al-P plants were well distributed through the medium. There was no obvious difference in impatiens root distribution. When plants at the marketing stage were exposed to drought, the Al-P plants of both species wilted more slowly than the conventionally fertilized controls. The reduced leaf area in both species and the improved root distribution of marigold may account for the improvement in drought tolerance of the Al-P plants.
Slicing Softly with Shear (2012)

A soft solid is more easily sliced using a combination of normal and shearing deformations rather than diced by squeezing down on it normally with the same knife. To explain why this is so, we experimentally probe the slicing and dicing of a soft agar gel with a wire, and complement this with theory and numerical simulations of cutting of a highly deformable solid. We find that purely normal deformations lead to global deformations of the soft solid, so that the blade has to penetrate deeply into the sample, well beyond the linear regime, to reach the relatively large critical stress to nucleate fracture. In contrast, a slicing motion leads to fracture nucleation with minimal deformation of the bulk and thus a much lower barrier. This transition between global and local deformations in soft solids as a function of the angle of shear explains the mechanics of the paper cut and design of guillotine blades.