gebhart/patrepeval huggingface dataset:
Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure (2007)

Pharmacologic activation of the heterodimeric HIF transcription factor appears promising as a strategy to treat diseases, such as anemia, myocardial infarction, and stroke, in which tissue hypoxia is a prominent feature. HIF accumulation is normally linked to oxygen availability because an oxygen-dependent posttranslational modification (prolyl hydroxylation) marks the HIFalpha subunit for polyubiquitination and destruction. Three enzymes (PHD1, PHD2, and PHD3) capable of catalyzing this reaction have been identified, although PHD2 (also called Egln1) appears to be the primary HIF prolyl hydroxylase in cell culture experiments. We found that conditional inactivation of PHD2 in mice is sufficient to activate a subset of HIF target genes, including erythropoietin, leading to striking increases in red blood cell production. Mice lacking PHD2 exhibit premature mortality associated with marked venous congestion and dilated cardiomyopathy. The latter is likely the result of hyperviscosity syndrome and volume overload, although a direct effect of chronic, high-level HIF stimulation on cardiac myocytes cannot be excluded.
RNAi and Cellular miRNAs in Infections by Mammalian Viruses (2011)

MicroRNAs (miRNAs) play an essential role in the regulation of eukaryotic gene expression. Recent studies demonstrate that miRNAs can also strongly affect the replication of pathogenic viruses. For example, cellular miRNAs can target and repress the expression of viral mRNAs, but there is also at least one example of a cellular miRNA that stimulates virus replication. Furthermore, viruses can encode their own miRNAs, trigger changes in cellular miRNA expression or encode RNA silencing suppressor factors that inhibit cellular miRNAs. These interactions together form a complex regulatory network that controls both viral and host gene expression, which ultimately determines the outcome of viral infection at the cellular level and disease progression in the host. Here, we summarize the literature data on such virus-cell interactions in mammals and discuss how miRNAs can be used as research tools or targets in the development of novel antiviral therapeutics.
High Levels of Circulating CXC Chemokine Ligand 10 Are Associated with Chronic Autoimmune Thyroiditis and Hypothyroidism (2004)

CXC chemokine ligand 10 (CXCL10), an interferon-gamma-inducible chemokine associated with Th1-mediated immune responses, has been proposed as a marker of inflammation in autoimmune diseases. We measured serum CXCL10 concentrations in 223 consecutive patients with newly diagnosed autoimmune thyroiditis (AT), 97 euthyroid controls, and 29 patients with nontoxic multinodular goiter and related this parameter to the clinical phenotype. The three groups were similar in gender distribution and age; among the AT patients, 24% had subclinical hypothyroidism. Serum CXCL10 level was significantly higher in AT patients (157 +/- 139 pg/ml) than in controls (79 +/- 38) or patients with multinodular goiter (90 +/- 32; P < 0.0001). Among patients with AT, CXCL10 levels were significantly higher in those with a hypoechoic ultrasonographic pattern and hypothyroidism. In a multiple linear regression model including age, thyroid volume, hypoechogenicity, hypervascularity, TSH, free T(4), and antithyroid peroxidase, only age (standardized coefficient = 0.39; P = 0.0001) and TSH (standardized coefficient = 0.41; P < 0.0002) were significantly related to serum CXCL10 levels. We conclude that circulating CXCL10 is increased in patients with AT and is associated with hypothyroidism. CXCL10 may be regarded as a marker of a more aggressive thyroiditis leading to thyroid destruction.
Lipoprotein-associated phospholipase A(2) as an independent predictor of coronary heart disease (2000)

Background: Chronic inflammation is believed to increase the risk of coronary events by making atherosclerotic plaques in coronary vessels prone to rupture. We examined blood constituents potentially affected by inflammation as predictors of risk in men with hypercholesterolemia who were enrolled in the West of Scotland Coronary Prevention Study, a trial that evaluated the value of pravastatin in the prevention of coronary events. Methods: A total of 580 men who had had a coronary event (nonfatal myocardial infarction, death from coronary heart disease, or a revascularization procedure) were each matched for age and smoking status with 2 control subjects (total, 1160) from the same cohort who had not had a coronary event. Lipoprotein-associated phospholipase A(2), C-reactive protein, and fibrinogen levels and the white-cell count were measured at base line, along with other traditional risk factors. The association of these variables with the risk of coronary events was tested in regression models and by dividing the range of values according to quintiles. Results: Levels of C-reactive protein, the white-cell count, and fibrinogen levels were strong predictors of the risk of coronary events; the risk in the highest quintile of the study cohort for each variable was approximately twice that in the lowest quintile. However, the association of these variables with risk was markedly attenuated when age, systolic blood pressure, and lipoprotein levels were included in multivariate models. Levels of lipoprotein-associated phospholipase A(2) (platelet-activating factor acetylhydrolase), the expression of which is regulated by mediators of inflammation, had a strong, positive association with risk that was not confounded by other factors. It was associated with almost a doubling of the risk in the highest quintile as compared with the lowest quintile. Conclusions: Inflammatory markers are predictors of the risk of coronary events, but their predictive ability is attenuated by associations with other coronary risk factors. Elevated levels of lipoprotein-associated phospholipase A(2) appear to be a strong risk factor for coronary heart disease, a finding that has implications for atherogenesis and the assessment of risk.
Spirulina Extract Gets FDA Approval for Coloring of Tablet and Capsule Coatings (2015)

U.S. dietary supplement manufacturers now have a new colorant option for creating naturally blue, green, and purple tablets or capsules.
Proceedings (Supplement), AFCRL Scientific Balloon Symposium (8th) 30 September to 3 October 1974 (1974)

Abstract : This publication, in conjunction with the main volume, AFCRL-TR-74- 0393, dated 21 August 1974, contains the papers presented at the Eighth AFCRL Scientific Balloon Symposium, 30 September to 3 October 1974, held at Hyannis, MA. The papers are grouped in accordance with the five symposium sessions: Powered Balloons, Tethered Balloons, Free Balloon Technology, Balloon-Borne Experiments, and Special Applications.
A Low-Noise Amplifier with Integrated Current and Power Sensors for RF BIST Applications (2007)

In this paper, the design and experimental results of the fully integrated CMOS current sensors and power sensors for RF built-in self-test (BIST) applications are presented. By utilizing a standard 0.18-mum CMOS process, a 5-GHz RF LNA incorporated with the on-chip sensors is fabricated for demonstration. With the proposed BIST technique, the circuit parameters of the LNA including the dc current, forward gain and gain compression can be extracted while maintaining only negligible performance degradation for the device under test (DUT) at multi-gigahertz frequencies.
Acrylic acid polymer film chemically modified graphite electrodes (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcrylic acid polymer film chemically modified graphite electrodesGeorge H. Heider, Mark B. Gelbert, and Alexander M. YacynychCite this: Anal. Chem. 1982, 54, 2, 322–324Publication Date (Print):February 1, 1982Publication History Published online1 May 2002Published inissue 1 February 1982https://pubs.acs.org/doi/10.1021/ac00239a042https://doi.org/10.1021/ac00239a042research-articleACS PublicationsRequest reuse permissionsArticle Views145Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
How to avoid bias when comparing bone marrow transplantation with chemotherapy. (1991)

It is important to know whether the survival of patients receiving an allogeneic bone marrow transplant (BMT) is better than that of patients receiving "conventional" treatment and, if BMT is better, to know how much better. Unfortunately, this information is surprisingly difficult to obtain accurately. Most studies that have attempted to define the value of BMT have been subject to varying degrees of bias because of the problems of identifying a "conventional chemotherapy" control group with which the outcome of patients who have received BMTs can be compared. A common bias arises when disease-free survival of BMT patients is compared with that of all other remitters. Early failures are then automatically assigned to the chemotherapy group even if they have donors and so would have gone on to a BMT had they not relapsed or died. Since some patients receive BMTs many months (or even years) after achieving remission-when their prognosis is already much improved-the definition of "early failure" is problematic. Nevertheless, although it is very rarely used, an adequate statistical method does exist to overcome this problem. Careful analysis cannot, however, overcome the problem of selection bias: patients selected for BMT are likely to have better (or worse) prognosis than patients who are treated conventionally. The only really satisfactory way of assessing the value of BMT is to conduct randomised trials comparing BMT with no BMT - or with extra chemotherapy. Several such studies are currently being undertaken assessing the role of autologous BMT in AML.(ABSTRACT TRUNCATED AT 250 WORDS)
New Code Acquisition Techniques in Spread-Spectrum Communication (1980)

New techniques for acquiring the codes used in frequency hopping (FH), hybrid frequency hopping/time hopping (FH/TH), and frequency hopping/direct sequence (FH/DS) spread-spectrum systems are presented. Autoregressive spectral estimation is employed to recover the FH code, quadrature processing is used for the DS code, while the TH gating code is recovered by a simple threshold test used in conjunction with the adaptive filter used for the spectral estimation. A wide margin of spectral estimation errors can be tolerated since an acquisition logic is used to estimate the FH code phase. The proposed techniques require acquisition times only of the order of the shift-register generator lengths, whereas other schemes of serial and parallel search require acquisition times of the order of the code lengths. Detailed simulation results will identify the working carrier-to-noise ratio (CNR) range for the different techniques presented as well as the various thresholds involved.
DEGRADATION OF THE CELL‐WALL POLYSACCHARIDE OF <i>PORPHYRIDIUM</i> SP. (RHODOPHYTA) BY MEANS OF ENZYMATIC ACTIVITY OF ITS PREDATOR, <i>GYMNODINIUM</i> SP. (PYRROPHYTA)<sup>1</sup> (1992)

ABSTRACT The dinoflagellate Gymnodinium sp., which preys specifically on cells of the red microalga Porphyridium sp., possesses enzymes that degrade exocellular polysaccharides of the Porphyridium sp. A crude extract of Gymnodinium sp. was applied to this polysaccharide, and the degradation products were characterized by charge and size separations. Charge separation revealed the presence of a fraction that was not found in the native polysaccharide. This fraction, which was eluted from an anion‐exchange resin with water alone, was composed mostly of glucose and xylose (in a 1:1 weight ratio). Size separation of the degradation products revealed three fractions; the molecular weight of the main one was 5 × 10 6 daltons, whereas that of the native polysaccharide was 7 × 10 6 daltons. The carbohydrate composition of these fractions was determined. Although the main product of degradation had a relatively high molecular weight, its viscosity was significantly reduced relative to the native polysaccharide. Additional enzymatic degradation is required for further exploration of the structure of the exocellular polymer of Porphyridium sp.
Solid phase immunoadsorption for therapeutic and analytical studies on neuropathy-associated anti-GM1 antibodies (2006)

Autoimmune neuropathies including Guillain-Barré syndrome are frequently associated with anti-GM1 ganglioside antibodies. These are believed to play a pathogenic role and their clearance from the circulation would be predicted to produce therapeutic benefit. This study examines the conditions required for effective immunoadsorption of anti-GM1 antibodies using glycan-conjugated Sepharose as a matrix. In solution inhibition studies using a range of GM1-like saccharides in conjunction with mouse and human anti-GM1 antibodies, the whole GM1 pentasaccharide beta-Gal-(1-3)-beta-GalNAc-(1-4)-[alpha-Neu5Ac-(2-3)]-beta-Gal-(1-4)-beta-Glc was the favored ligand for maximal inhibiton of antibody-GM1 interactions in comparison with monosaccharides, Gal-(1-3)-beta-GalNAc-betaOMe, and synthetic GM1 mimetics. Immunoadsorption studies comparing binding of mouse monoclonal anti-GM1 antibodies to GM1-Sepharose and beta-Gal-(1-3)-beta-GalNAc-Sepharose confirmed the preference seen in solution inhibition studies. GM1-Sepharose columns were then used to adsorb anti-GM1 immunoglobulin G and immunoglobulin M antibodies from human neuropathy sera. Anti-GM1 antibodies subsequently eluted from the columns often showed a striking monoclonal or oligoclonal pattern, indicating that the immune response to GM1 is restricted to a limited number of B-cell clones, even in the absence of a detectable serum paraprotein. These data support the view that immunoadsorption plasmapheresis could potentially be developed for the acute depletion of serum anti-GM1 antibodies in patients with neuropathic disease, and also provide purified human anti-GM1 antibodies for analytical studies.
Purification and Characterization of<i>α</i>-Keto Ester Reductases from Bakers’ Yeast (1994)

Three α-keto ester reductases (yeast keto ester reductase, YKER-II, -IV, -V) were purified from bakers’ yeast. YKER-II, -IV, and -V are dimeric, monomeric, and dimeric enzymes, respectively, and molecular masses are estimated to be 58, 31–39, and 83kDa, respectively, based on gel filtration and SDS- polyacrylamide electrophoresis. Kinetic parameters and stereoselectivities in reduction of α-keto esters have been measured. YKER-IV contributes mainly to reduction by bakers’ yeast at low substrate concentrations, and is useful for synthetic purposes.
Novel Anode Catalyst for Direct Methanol Fuel Cells (2014)

PtRu catalyst is a promising anodic catalyst for direct methanol fuel cells (DMFCs) but the slow reaction kinetics reduce the performance of DMFCs. Therefore, this study attempts to improve the performance of PtRu catalysts by adding nickel (Ni) and iron (Fe). Multiwalled carbon nanotubes (MWCNTs) are used to increase the active area of the catalyst and to improve the catalyst performance. Electrochemical analysis techniques, such as energy dispersive X-ray spectrometry (EDX), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and X-ray photoelectron spectroscopy (XPS), are used to characterize the kinetic parameters of the hybrid catalyst. Cyclic voltammetry (CV) is used to investigate the effects of adding Fe and Ni to the catalyst on the reaction kinetics. Additionally, chronoamperometry (CA) tests were conducted to study the long-term performance of the catalyst for catalyzing the methanol oxidation reaction (MOR). The binding energies of the reactants and products are compared to determine the kinetics and potential surface energy for methanol oxidation. The FESEM analysis results indicate that well-dispersed nanoscale (2-5 nm) PtRu particles are formed on the MWCNTs. Finally, PtRuFeNi/MWCNT improves the reaction kinetics of anode catalysts for DMFCs and obtains a mass current of 31 A g(-1) catalyst.
Synthesis and characterization of a functional intact IgG in a prokaryotic cell-free expression system (2007)

Antibodies are an important component of the immune system of higher eukaryotes. Furthermore, they are effective tools in basic research, medical diagnostics and therapy. Recombinant expression of these heterotetrameric, disulfide-bridged proteins is usually performed in mammalian cells. Here, we describe the cell-free expression of a mouse monoclonal antibody, MAK33, in a coupled transcription/translation system, based on an Escherichia coli lysate. Both the heavy and the light chain can be produced efficiently in this setup. However, they fail to form functional antibodies. With a view to overcome folding and oxidation defects, we supplemented the system with the oxidoreductases PDI (protein disulfide isomerase) and DsbC and the ER-specific chaperones Grp94 and BiP; furthermore, we optimized the redox conditions. We found that functional antibodies can only be obtained in the presence of an oxidoreductase. In contrast, the addition of Grp94 and/or BiP had no influence on the productive folding reaction. The comparison of the antibody expressed in vitro with MAK33 expressed in cell culture showed that the in vitro expressed antibody is correctly assembled, disulfide-bridged and shows identical antigen affinity. The stability of the in vitro expressed non-glycosylated IgG is comparable to that of the authentic antibody.
Effects of Duct Lip Shaping and Various Control Devices on the Hover and Forward Flight Performance of Ducted Fan UAVs (2005)

The military's desire for ducted fan vertical takeoff and landing (VTOL) unmanned aerial vehicles (UAVs) stems from the vehicles' relatively small size, safety in tight quarters, increased payload capacity for their size, and their ability to hover for surveillance missions. However, undesirable aerodynamic characteristics are associated with these vehicles in crosswinds, namely momentum drag and asymmetric duct lift. Because the duct itself, and not the fan, is the root cause of these unfavorable aerodynamic attributes, various lip shapes were tested to determine the effects of leading edge radius of curvature and duct wall thickness. It was found that a lip with a small leading edge radius performed best in forward flight and crosswind conditions, while the performance of a lip with a large leading edge radius was enhanced in static conditions. Through tuft flow visualization and static pressure measurements it was determined that the reason for the difference in performance between the two lips was due to flow separation on the interior of the duct lip surface. Control vanes positioned aft of the duct were tested as the primary attitude control for the vehicle. An empirical control vane model was created based on the static data for the control vanes, and it was applied to wind tunnel test results to determine the required control vane angle for trim. Wind tunnel testing showed the control vanes were capable of trimming out the adverse pitching moment generated by the duct, but at some flight speeds large vane deflections were necessary. Additional control devices placed at the lip of the duct and stabilizer vanes positioned aft of the duct were tested to reduce the amount of control vane deflection required for trim. It was found that the duct deflector control effector had the largest impact on the adverse pitching moment, while the stabilizer vanes were only effective at low crosswind velocities.
High magnetic permeability of ε-GaxFe2−xO3 magnets in the millimeter wave region (2010)

In this study, the magnetic permeability of a series of metal substituted ε-Fe2O3 was first measured. The electromagnetic wave absorption properties were measured in the region of 50–75 GHz (V band), and the materials exhibited absorption peaks at 64 GHz (ε-Ga0.51Fe1.49O3), 59 GHz (ε-Ga0.56Fe1.44O3), and 55 GHz (ε-Ga0.61Fe1.39O3). From the reflectance and transmittance measurements, the magnetic permeability (μ′-jμ″) and dielectric constant (ε′-jε″) of ε-GaxFe2−xO3 were evaluated. The μ″ values of ε-GaxFe2−xO3 are the highest values among reported magnetic millimeter wave absorbers in the V-band region.
Vibration control using an adaptive tuned vibration absorber with a variable curvature stiffness element (2005)

An adaptive tuned vibration absorber (ATVA) with a variable stiffness element is capable of retuning itself in response to a time-varying excitation frequency, enabling effective vibration control over a range of frequencies. One way of adjusting the stiffness is by shape change, and this paper presents the design for an ATVA that exploits this principle. The stiffness element is formed from parallel curved beams whose curvature can be adjusted and hence the stiffness can be changed in real time. The performance of a prototype ATVA with piezo-actuated curved beams is analysed and good correlation is achieved between theoretical and experimental results. The device is shown to be tunable over the frequency range 36–56 Hz, with the maximum tuned frequency being limited by inertia effects of the curved beams. The tests demonstrate the efficacy of this design in vibration control, particularly with respect to its agility in tracking rapidly varying forcing frequencies.
Nonstoichiometry and electrocoloration due to injection of Li+ and O2− ions into lithium niobate crystals (2000)

The high oxygen and lithium ion conductivity in LiNbO3 was investigated and interpreted in terms of lithium and oxygen vacancies being intrinsically present in congruently grown single crystals. As a result of this, it was found that the stoichiometry of lithium niobate crystals may be changed with respect to lithium and oxygen. The optical and electrical properties of electrically colored LiNbO3 crystals were studied and it was shown that the absorption spectra of thermally reduced and electrocolored samples are identical. Therefore, the origin of the absorption processes is considered to be the same in both cases. The formation of regions with different stoichiometry due to the injection of additional lithium or oxygen into the LiNbO3 crystals was also observed and investigated. The motion of these stoichiometric domains through a LiNbO3 crystal from one electrode to the other was studied and described in terms of electrodiffusion of the ions and electrons. A model is proposed which considers the injection of oxygen and lithium vacancies for the generation of concentration profiles in the originally homogeneous material. The numerical calculation of the concentration profiles shows good agreement with the experimental results.
On Testing and Evaluating Service-Oriented Software (2008)

As service-oriented architecture matures and more Web services become available, developers must test an ever-increasing volume of services. A framework that defines and evaluates test-case potency based on coverage relationships can reduce testing effort while maintaining testing's effectiveness.