gebhart/patrepeval huggingface dataset:
Cloning,expression and purification of the M gene from SARS Coronavirus (2005)

To clone the M gene of SARS Coronavirus,express in E.coli and purify the expression products,the M gene fragment of SARS Coronavirus was amplified and cloned into the pMD-18T vector.After nucleotide sequencing,the M gene fragment was subcolned into the expression vector pET23a with correct orientation and then transformed into E.coli DH5α.The plasmid from the corrected clone identified by PCR and endonuclease digestion was transformed into E.coli BL21(DE3)and then induced for expression.The expression product was purified by metal-chelated affinity chromatography and analyzed by Western blotting.It was found that the 675bp gene fragment was amplified by RT-PCR.Nucleotide sequencing showed there was no sense mutation.A 27KD protein was expressed after induction and purified by metal-chelated affinity Chromatography.Western blotting result indicated that the expressed products could be recognized by sera of SARS Patinats.M gene of SARS Coronavirus was successively cloned and expressed in E.coli and the expressed gene products were successfully purified and recognized by SARS patient's is serum.
Near-Infrared Resonance Raman Spectroscopy of the Special Pair and the Accessory Bacteriochlorophylls in Photosynthetic Reaction Centers (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNear-Infrared Resonance Raman Spectroscopy of the Special Pair and the Accessory Bacteriochlorophylls in Photosynthetic Reaction CentersNerine J. Cherepy, Andrew P. Shreve, Laura J. Moore, Stefan Franzen, Steven G. Boxer, and Richard A. MathiesCite this: J. Phys. Chem. 1994, 98, 23, 6023–6029Publication Date (Print):June 1, 1994Publication History Published online1 May 2002Published inissue 1 June 1994https://pubs.acs.org/doi/10.1021/j100074a032https://doi.org/10.1021/j100074a032research-articleACS PublicationsRequest reuse permissionsArticle Views268Altmetric-Citations102LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Oleylamine-Mediated Synthesis of Pd Nanoparticles for Catalytic Formic Acid Oxidation (2009)

We report a facile synthesis of monodisperse Pd nanoparticles by the reduction of Pd(acac)(2) with oleylamine and borane tributylamine complex. The oleylamine-coated Pd nanoparticles are readily "cleaned" with a 99% acetic acid wash, and the Pd particles supported on Ketjen carbon are catalytically active for formic acid oxidation in HClO(4) solution. The catalyst shows no obvious activity degradation after 1500 cyclic voltammetry cycles under ambient conditions. These Pd particles hold promise as a highly active non-Pt catalyst for fuel cell applications.
The effect of collisional broadening on Monte Carlo simulations of high-field transport in semiconductor devices (1981)

Some important consequences of the uncertainty principle on Monte Carlo simulations of very high field transport are discussed. It is shown that recent values of the phonon scattering rates reported for GaAs by Shichijo and Hess lead to an unrealistically high collisional broadening (0.3-0.6eV) of the electronic states, thus rendering questionable any attempt to relate transport properties to the band structure, and invalidating the semiclassical Boltzmann transport picture used in the Monte Carlo simulation. These considerations are important in the modeling of very high field transport properties in semiconductor devices.
COMPARATIVE STUDY OF CLINICAL FACTORS THAT PREDISPOSE PATIENTS TO PROLIFERATIVE VITREORETINOPATHY IN APHAKIA (1991)

To clarify the risk factors of proliferative vitreoretinopathy (PVR) in aphakia, the clinical features of 25 aphakic eyes with PVR were statistically analyzed and compared with a control group of 157 aphakic eyes with non-PVR rhegmatogenous retinal detachment. The statistically significant (P less than 0.05) factors that predisposed patients to PVR in aphakia were as follows: a history of vitreous loss on cataract surgery, retinal detachment developing within 3 months after cataract extraction, duration of retinal detachment longer than 3 months, break larger than three disc diameters, and choroidal detachment. Vitreous loss is believed to play the most important role in the development of PVR in aphakia.
Angiotensinogen and Megalin Interactions Contribute to Atherosclerosis—Brief Report (2018)

Objective- AGT (Angiotensinogen) is the unique precursor of the renin-angiotensin system that is sequentially cleaved by renin and ACE (angiotensin-converting enzyme) to produce Ang II (angiotensin II). In this study, we determined how these renin-angiotensin components interact with megalin in kidney to promote atherosclerosis. Approach and Results- AGT, renin, ACE, and megalin were present in the renal proximal convoluted tubules of wild-type mice. Hepatocyte-specific AGT deficiency abolished AGT protein accumulation in proximal tubules and diminished Ang II concentrations in kidney, while renin was increased. Megalin was most abundant in kidney and exclusively present on the apical side of proximal tubules. Inhibition of megalin by antisense oligonucleotides (ASOs) led to ablation of AGT and renin proteins in proximal tubules, while leading to striking increases of urine AGT and renin concentrations, and 70% reduction of renal Ang II concentrations. However, plasma Ang II concentrations were unaffected. To determine whether AGT and megalin interaction contributes to atherosclerosis, we used both male and female low-density lipoprotein receptor<sup>-/-</sup> mice fed a saturated fat-enriched diet and administered vehicles (PBS or control ASO) or megalin ASO. Inhibition of megalin did not affect plasma cholesterol concentrations, but profoundly reduced atherosclerotic lesion size in both male and female mice. Conclusions- These results reveal a regulatory role of megalin in the intrarenal renin-angiotensin homeostasis and atherogenesis, positing renal Ang II to be an important contributor to atherosclerosis that is mediated through AGT and megalin interactions.
Continuous long-term culture of human bone marrow (1981)

Human bone marrow was cultured with alpha medium, 10(-6)M hydrocortisone and a mixture of horse and fetal calf serum in a long-term liquid culture system. An adherent layer was formed with contained fat cells, macrophages, fibroblast-like and epithelioid cells. The layer harboured myeloid cells and their presumptive precursors whilst the nonadherent cells were composed of immature myeloid elements plus mature macrophages and granulocytes whose numbers were maintained for periods of up to 12 weeks. Experiments showed that the use of serum mixtures was superior to horse or fetal calf serum alone and successful culture was accompanied by early growth of the hydrocortisone-dependent fat cells in the adherent layer.
Experimental approach to the mechanism of the negative bias enhanced nucleation of diamond on Si via hot filament chemical vapor deposition (1996)

The mechanism of the negative bias enhanced nucleation of diamond on silicon has been studied by a set of experiments using hot filament chemical vapor deposition. Nucleation enhancement was achieved for experimental configurations either with or without the application of a negative bias to the mirror-polished Si substrates. The obtained nucleation density ranged from 108 to 1010 cm−2. The as-deposited films were characterized by scanning electron microscopy and Raman spectroscopy. Our results demonstrate that the electron emission from the diamond coating the substrate holder, which is speculated to greatly increase the concentration of atomic hydrogen and dissociated hydrogen radicals on/near the substrate surface is responsible for the nucleation enhancement during the bias pretreatment.
Duration of feeding and superficial and in-depth damage to soybean seed by selected species of stink bugs (Heteroptera: Pentatomidae) (2011)

Laboratory studies were conducted to compare duration of feeding and superficial and in-depth damage to soybean (Glycine max) seeds by the Southern green stink bug, Nezara viridula (L.), the Neotropical brown stink bug, Euschistus heros (F.), the red-banded stink bug, Piezodorus guildinii (Westwood), and the green-belly stink bug, Dichelops melacanthus (Dallas). Results indicated that feeding time was significantly longer for N. viridula (≈ 133 min) compared to E. heros and D. melacanthus (≈ 70 min), but not different from P. guildinii (≈ 103 min). There was a positive correlation between feeding time and the resulting damage for E. heros, N. viridula and P. guildinii (R² > 0.80, P < 0.0001), but not for D. melacanthus (R² = 0.1011, P = 0.1493). The deepest seed damage (2.0 mm) was made by P. guildinii and the shallowest (0.5 mm) by D. melacanthus. The depth of the seed damage by E. heros and N. viridula (0.8, 1.2 mm, respectively) was intermediate in comparison to the other species studied. Feeding damage to the seed endosperm caused variable cell disruption and protein body dissolution, particularly when P. guildinii fed on seeds, suggesting that the deleterious action of salivary enzymes was greater for this bug compared to the others.
Low-power microelectronics embedded in live jellyfish enhance propulsion (2020)

Artificial control of animal locomotion has the potential to simultaneously address longstanding challenges to actuation, control, and power requirements in soft robotics. Robotic manipulation of locomotion can also address previously inaccessible questions about organismal biology otherwise limited to observations of naturally occurring behaviors. Here, we present a biohybrid robot that uses onboard microelectronics to induce swimming in live jellyfish. Measurements demonstrate that propulsion can be substantially enhanced by driving body contractions at an optimal frequency range faster than natural behavior. Swimming speed can be enhanced nearly threefold, with only a twofold increase in metabolic expenditure of the animal and 10 mW of external power input to the microelectronics. Thus, this biohybrid robot uses 10 to 1000 times less external power per mass than other aquatic robots reported in literature. This capability can expand the performance envelope of biohybrid robots relative to natural animals for applications such as ocean monitoring.
Absolute Beginner's Guide to Corel WordPerfect 10 (2003)

From the Publisher:
In recent news, Corel announced that HP, the world's largest PC manufacturer, Dell, the second-largest, and Sony have all agreed to preinstall WordPerfect 10 and Quattro Pro 10 on some of their computer lines. Cost differential between Microsoft's Office and Works Suites, as well as Microsoft's Office licensing terms is helping fuel the trend to switch software vendors. With more consumers using this product, the need for a beginner-level book on these software programs has increased as well, and the Absolute Beginner's Guide to Corel WordPerfect 10 is the only such resource available! The book assumes absolutely no prior knowledge of the topic, and the information is presented in an easy-to-read, lighthearted style. Bonus chapters on QuattroPro 10 and TaskManager appear in the book and other material is available on www.quepublishing.com. This title is for those who are new to WordPerfect and want to learn how to use the word processing program from start to finish. It is also a great guide for users who are upgrading from previous versions of the software and want to know the newest features.
Targeting the Assembly of Bacterial Cell Division Protein FtsZ with Small Molecules (2011)

FtsZ is the key protein of bacterial cell division and an emergent target for new antibiotics. It is a filament-forming GTPase and a structural homologue of eukaryotic tubulin. A number of FtsZ-interacting compounds have been reported, some of which have powerful antibacterial activity. Here we review recent advances and new approaches in modulating FtsZ assembly with small molecules. This includes analyzing their chemical features, binding sites, mechanisms of action, the methods employed, and computational insights, aimed at a better understanding of their molecular recognition by FtsZ and at rational antibiotic design.
Instrumentation Techniques for Low Voltage Impulse Testing of Power Transformers (1972)

Bonneville Power Administration' s instrumentation ion techniques for the Low Voltage Impulse method of detecting physical internal changes in transformers is presented. The potential use of the method for locating that movement is also discussed.
Hyaluronan affects extravascular water in lungs of unanesthetized rabbits (1989)

We have determined whether changes in lung hyaluronan content affect extravascular water in lungs of unanesthetized rabbits. Three groups of experiments were performed. In group 1 (n = 12), no infusions were given; in group 2, nine pairs of rabbits received either intravenous hyaluronidase (750 U.kg-1.min-1) or an equivalent volume of saline; in group 3, nine pairs of rabbits received either hyaluronidase or saline, followed by intravenous saline infusion amounting to 24% of body weight. At the end of each experiment, one lung was analyzed for extravascular lung water by the wet-dry method. Except for group 3, in all animals the other lung was analyzed for hyaluronan content by a method that involved hydrolyzing lung hyaluronan with fungal hyaluronidase to release reducing N-acetyl glucosamine groups, which were quantified. In group 1, lung hyaluronan, which varied from 50 to 159 micrograms/g dry wt (mean 106 +/- 35 micrograms/g dry wt), significantly correlated with variation in extravascular lung water (mean 4.2 +/- 0.3 g/g dry wt). In group 2 rabbits given hyaluronidase, lung hyaluronan was 40% lower and extravascular lung water was 14.6% lower than in paired controls (P less than 0.01). In group 3, volume expansion did not affect lung water, except after hyaluronidase when lung water was 47% higher than paired controls. We conclude that in the lung the content of hyaluronan is one of the determinants of extravascular water content.
Some applications of the Eulerian functions of a finite group (1961)

This paper is chiefly concerned with inequalities for the numbers of subgroups of a finite p -group. The following are typical results. Let G be a p -group of order p a , and let n G (p k ) denote the number of subgroups of G of order p k .
SQLoop: High Performance Iterative Processing in Data Management (2018)

Increasingly more iterative and recursive query tasks are processed in data management systems, such as graph-structured data analytics, demanding fast response time. However, existing CTE-based recursive SQL and its implementation ineffectively respond to this intensive query processing with two major drawbacks. First, its iteration execution model is based on implicit set-oriented terminating conditions that cannot express aggregation-based tasks, such as PageRank. Second, its synchronous execution model cannot perform asynchronous computing to further accelerate execution in parallel. To address these two issues, we have designed and implemented SQLoop, a framework that extends the semantics of current SQL standard in order to accommodate iterative SQL queries. SQLoop interfaces between users and different database engines with two powerful components. First, it provides an uniform SQL expression for users to access any database engine so that they do not need to write database dependent SQL or move datasets from a target engine to process in their own sites. Second, SQLoop automatically parallelizes iterative queries that contain certain aggregate functions in both synchronous and asynchronous ways. More specifically, SQLoop is able to take advantage of intermediate results generated between different iterations and to prioritize the execution of partitions that accelerate the query processing. We have tested and evaluated SQLoop by using three popular database engines with real-world datasets and queries, and shown its effectiveness and high performance.
Similar role of waveguide bends in photonic crystal circuits and disordered defects in coupled cavity waveguides: An intrinsic problem in realizing photonic crystal circuits (2003)

We investigate, experimentally and theoretically, the influence of disordered defects on the transmission properties of optical delay lines based on coupled cavity waveguides (CCW's). Also, the modification of transmission for a line-defect waveguide in a two-dimensional (2D) photonic crystal (PC) upon bending is examined by numerical simulation. We reveal that the effect of waveguide bending on the transmission properties of PC circuits is very similar to that of disordered defects on the transmission properties of CCW's. It is shown that the broad impurity band of a one-dimensional CCW will evolve into sharp defect modes upon increasing the disorder. Using a symmetric Mach-Zehnder structure formed in a 2D PC as an example, we show that a PC circuit containing multiple bends and branches of different properties generally gives rise to sharp resonant modes whose linewidth is inversely proportional to the size of the PC circuit. This might be an intrinsic problem in realizing PC circuits.
On-target and Off-target-based Toxicologic Effects (2012)

Adverse toxicologic effects are categorized as chemical-based, on-target, or off-target effects. Chemical-based toxicity is defined as toxicity that is related to the physicochemical characteristics of a compound and its effects on cellular organelles, membranes, and/or metabolic pathways. On-target refers to exaggerated and adverse pharmacologic effects at the target of interest in the test system. Off-target refers to adverse effects as a result of modulation of other targets; these may be related biologically or totally unrelated to the target of interest. Both the risk assessment and development strategies used for xenobiotics are influenced by the understanding of the mechanism of toxicity. It is imperative that the toxicologic pathologist use the toxicologic and biologic data at hand and literature information on the target to form testable hypotheses related to whether a toxicity is chemical-based, on-target, or off-target. The objective of this session at the 2012 Society of Toxicologic Pathologists Symposium in Boston, Massachusetts, was to discuss chemical-based, on-target, and off-target-based effects and the scientific approaches used to aid in their human risk assessment.
Static And Dynamic Properties Of Active Joints (2005)

In this paper the realisation and the first experimental results of a new electrostatic actuator, the 'active joint' are presented. This structure is capable of delivering large forces and large deflections. When a sinusoidal voltage is applied with an amplitude larger than the critical voltage and a frequency close to the resonant frequency, the active joints shows a chaotic response. A first model is presented in order to describe the dynamic behaviour of active joints.
The Rule of Law as Transition to Democracy in China (2003)

This paper assesses Pan Wei's proposal for a 'consultative rule of law system' for China, finding it a potentially important step along the path of political reform. China urgently needs political reform to deal with the rapidly mounting problems of corruption, abuse of power, financial scandals, rising crime and inequality, and declining legitimacy of the Chinese Communist Party. A rule of law, with an independent judiciary and other autonomous institutions of horizontal accountability, is vital if China is to rein in these problems and deliver better, fairer, more transparent and effective governance. However, Pan Wei's proposed system goes only part of the way toward addressing the deficiencies of governance in China, and is therefore best viewed as a transitional framework. To work, horizontal accountability must be supplemented with and reinforced by vertical account ability, through competitive elections, which give local officials an incentive to serve the public good and enable bad officials to be removed by the people. Ultimately, I argue, China can only achieve adequate and enduring political accountability by moving toward democracy. Among the other issues addressed in the paper are the architecture and appointment of a system of horizontal accountability for China; the role of the Communist Party (or its successor hegemon) in a 'rule of law' system; and the timing and phasing of the transition to a rule of law.
Various applications of direct PCR using blood samples. (2002)

Samples of blood or other animal fluids contain a variety of substances that inhibit the polymerase chain reaction (PCR), meaning that isolation of DNA, involving multiple labor-intensive steps, is generally necessary prior to PCR. We have developed a novel reagent cocktail that effectively suppresses these inhibitory substances. Using this reagent cocktail, DNA from various targets can be efficiently amplified directly from various forms of blood samples without DNA isolation. 1. DNA sequences within the beta-globin gene could be amplified directly from human blood samples treated with various anticoagulants. Either fresh blood or blood samples stored frozen for up to 4 years could be used for PCR. 2. DNA sequences of up to 2056 bp within the beta-globin gene could be amplified directly from human blood samples. 3. Human chromosomal and mitochondrial DNA from different individuals could be amplified directly from blood samples. 4. Low titers of hepatitis B virus could be amplified directly from human blood samples. 5. DNA could be amplified directly from various target sequences using dried blood in a PCR tube or on a filter paper. 6. Transgenes could be detected directly in blood samples from transgenic mice.
Metamaterials with negative permittivity and permeability: background, salient features, and new trends (2003)

Here the author presents a brief background and the history of complex media, in particular the materials with negative permittivity and permeability, and then he discusses some of the salient electromagnetic features of these metamaterials. This is followed by description of some of the ideas regarding potential future applications of these metamaterials in devices and components, along with physical remarks and intuitive justification.