gebhart/patrepeval huggingface dataset:
Increased nuclear conjugated polyamines and transglutaminase during liver regeneration. (1981)

The nuclear content of conjugated polyamines increased during rat liver regeneration. Conjugated polyamines isolated from the acid-precipitable fraction of nuclei required peptide bond hydrolysis for release of the parent compounds. The most striking change occurred in conjugated putrescine which fluctuated in a biphasic manner; maximal nuclear levels 12-fold and 25-fold above those of sham-operated controls were achieved at 4 and 42 hr after hepatectomy, respectively. Conjugated spermidine and spermine increased 3- and 2-fold respectively within 4 hr and remained high throughout the 48 hr studied. When expressed on the basis of mg of nuclear protein, the maximal conjugated putrescine increased 19-fold, conjugated spermidine increased 2-fold, and conjugated spermine decreased by 50%. Therefore, the spermidine and spermine conjugates may be of a more constitutive nature whereas the large changes in the nuclear conjugation of putrescine associated with the onset of growth may play a regulatory role. The nucleus also contained transglutaminase (R-glutaminyl-peptide:amine gamma-glutamyl-yltransferase, EC 2.3.2.13), an enzyme shown in vitro to conjugate polyamines covalently to proteins. The specific activity of the nuclear enzyme increased rapidly after partial hepatectomy to a level 3-fold above control at 4 hr and 7-fold above control at 42 hr. The increased conjugating activity resulted from an increase in detectable maximal velocity and not a change in affinity of the enzyme for putrescine (Km congruent to 0.4 mM). There was also a 3-fold increase at 42 hr in the number of nuclear amine acceptor sites present to which radiolabeled putrescine could be conjugated by endogenous enzyme.
Methods to extract the exopolymeric matrix from biofilms: a comparative study (1999)

To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization and the biofilm matrix is solubilized in the solution.
Silyloxyphosphanes. New Phosphitylating Reagents in Nucleotide Chemistry (1991)

Abstract Letsinger et al. developed a phosphite method of synthesis of oligonucleotides. This method was further improved by Carruthers et al. Our present work is a useful extension of the Letsinger-Carruthers type reagents. Recently, we have been able to demonstrate that bis- (N, N-diisopropylamino)trimethylsilyloxy phosphane exhibit phosphitylating properties in the presence 1-H-tetrazole.1 Preparation of this reagent and its ability to undergo selective nucleophilic substitution at the tricoordinate phosphorus atom without affecting the silicon center under conditions which are commonly used for the phosphite triester approach has been recently described.1 In the same paper we reported remarkable chemical properties of dinucleosidetrimethyl-siloxyphosphanes. Namely their participation in Michaelis-Arbuzov type reactions under extremely mild conditions and reactions with oxalylazolides and sulfuryl chloride fluoride which are unique for these reagents.
Quantitative Validation of Media for Transportation and Storage of <i>Streptococcus pneumoniae</i> (2003)

The need to design effective Streptococcus pneumoniae vaccines and to monitor resistance means that it is essential to have efficient methods to determine carriage rates. Two liquid media, consisting of skim milk, glycerol, glucose, and tryptone soya broth (STGG) or skim milk, glycerol, and glucose (SGG) alone, were evaluated for their ability to maintain pneumococcal viability. Optimal recovery of S. pneumoniae was achieved when swabs were transferred to STGG medium prior to plating onto blood agar-gentamicin selective plates (22%) compared to 7% when plated out directly (P < 0.0001 by Fisher's exact test). Both STGG and SGG media are appropriate for the long-term storage of pneumococci and primary swab samples at -70 degrees C, with no decrease in viable count observed following repeated freeze-thaw cycles. Samples could be stored refrigerated for up to 3 days in either STGG or SGG medium with no significant loss of viability. Viability decreased progressively in storage at 20 to 30 degrees C, with greater losses of viability occurring at the higher temperatures. There were no significant differences in viability between isolates in the two media. STGG preserved pneumococci significantly better (about twofold) than SGG medium at 21 degrees C (P < 0.0001) and 30 degrees C (P < 0.0001). Samples can be stored for 4 and 2.5 days at 6 to 8 degrees C, 28 and 17 h at 21 degrees C, and 15 and 7 h at 30 degrees C in STGG and SGG media, respectively. For field studies undertaken in resource-limited environments, SGG medium can be prepared by using locally available materials. The quantitative data reported in this study will enable researchers to plan appropriate transport and storage protocols.
Tighter Confinement Increases Selectivity of <scp>d</scp>‐Glucose Isomerization Toward <scp>l</scp>‐Sorbose in Titanium Zeolites (2020)

Aqueous-phase isomerization of d-glucose to d-fructose and l-sorbose is catalyzed in parallel by Lewis acidic Ti sites in siliceous frameworks. Glucose isomerization rates (per Ti, 373 K) are undetectable when Ti sites are confined within mesoporous voids (Ti-MCM-41, TiO<sub>2</sub> -SiO<sub>2</sub> ) and increase to detectable values when Ti sites are confined within the smaller 12-membered ring (12-MR) micropores of Ti-Beta. Isomerization rates decrease to lower values (by ≈20×) with further decreases in micropore size as Ti sites are confined within 10-MR pores (Ti-MFI, Ti-CON), likely because of intrapore reactant diffusion restrictions, and reach undetectable values within the 8-MR pores of Ti-CHA as size exclusion prevents glucose from accessing active sites. Remarkably, the selectivity toward l-sorbose over d-fructose increases systematically as spatial constraints around Ti sites become tighter, and is >10 on Ti-MFI. These findings demonstrate the marked influence of confinement around Ti active sites on the selectivity between parallel stereoselective sugar isomerization pathways.
The production of freeze-dried immobilized cultures of Streptococcus thermophilus and their acidification properties in milk (2001)

The aim of this study was to prepare alginate-immobilized freeze-dried cultures of Streptococcus thermophilus and to compare the acidifying activities of these rehydrated cultures with classical free cell liquid inoculants. Streptococcus thermophilus BT1 grew in alginate beads and the population reached 10(10) cfu g(-1) after 6 h incubation. Re-inoculation of the beads in fresh medium with a further 6 h incubation did not improve the biomass level, but extending the incubation at 42 degrees C to 24 h caused significant death. The rehydrated immobilized cell technology (ICT) starter contained 13% free cells. In acidifying activity tests, the ICT culture had a similar acidification curve to that of a classical milk-grown free cell culture, except that it reached lower final pH values. Although the differences between the ICT and liquid cultures were not important, there were significant effects of inoculation level on lag time, maximum acidification rate and on the pH and time at which the acidification rate was at its highest.
The expression profiles of miRNA–mRNA of early response in genetically improved farmed tilapia (Oreochromis niloticus) liver by acute heat stress (2017)

Genetically improved farmed tilapia (GIFT, Oreochromis niloticus) are commercially important fish that are cultured in China. GIFT are highly susceptible to diseases when exposed to high temperatures in summer. Better understanding the GIFT regulatory response to heat stress will not only help in determining the relationship between heat stress signalling pathways and adaption mechanisms, but will also contribute to breeding new high-temperature tolerant strains of GIFT. In this study, we built control (28 °C) and heat-treated (37.5 °C) groups, and extracted RNA from the liver tissues for high-throughput next-generation sequencing to study the miRNA and mRNA expression profiles. We identified 28 differentially expressed (DE) miRNAs and 744 DE mRNAs between the control and heat-treated groups and annotated them using the KEGG database. A total of 38 target genes were predicted for 21 of the DE miRNAs, including 64 negative miRNA-mRNA interactions. We verified 15 DE miRNA-mRNA pairs and 16 other DE mRNAs by quantitative real-time PCR. Important regulatory pathways involved in the early response of GIFT to heat stress included organism system, metabolism, and diseases. Our findings will facilitate the understanding of regulatory pathways affected by acute heat stress, which will help to better prevent heat damage to GIFT.
Privacy-preserving energy theft detection in smart grids (2012)

In the U.S., energy theft causes six billion dollar losses to utility companies (UCs) every year. With the smart grid being proposed to modernize current power grids, energy theft may become an even more serious problem since the “smart meters” used in smart grids are vulnerable to more types of attacks compared to traditional mechanical meters. Therefore, it is important to develop efficient and reliable methods to identify illegal users who are committing energy theft. Although some schemes have been proposed for the UCs to detect energy theft in power grids, they all require the users to send their private information, e.g., load files or meter readings at certain times, to the UCs which invades users' privacy and raises serious concerns about privacy, safety, etc. As far as we know, we are the first to investigate the energy theft detection problem considering users' privacy issues. In this paper, we propose to solve in a distributed fashion a linear system of equations (LSE) for the users' “honesty coefficients”, which indicate the users are honest when equal to 1 and are fraudulent when larger than 1. In particular, we develop two distributed privacy-preserving energy theft detection algorithms based on LU decomposition, called LUD and LUPD, respectively, which can identify fraudulent users without invading any user's privacy. Compared to LUD, LUPD requires higher execution time but is stable even in large-size systems. Moreover, the LUD and LUPD algorithms are proposed in the case that users commit energy theft at a constant rate, i.e., with constant honesty coefficients. We also propose adaptive LUD/LUPD algorithms to account for the scenarios where the users have variable honesty coefficients. Extensive simulations are carried out and the results show that the proposed algorithms can efficiently and successfully identify the fraudulent users in the system.
ChemInform Abstract: ORGANIC METAL COMPLEXES, XIII. ANISOTROPY EFFECTS OF CONJUGATED CYCLIC SYSTEMS, II. ANISOTROPY EFFECTS IN THE NMR SPECTRA OF METAL CHELATES OF 3‐MESITYL‐ AND 3‐(9‐ANTHRYL)ACETYLACETONE (1976)

Abstract Durch Erhitzen des Acetylacetonato‐Cr‐Komplexes (I) und Jodmesitylen bzw. Bromanthracen (II) mit Cu‐ Pulver werden das Mesityl‐ bzw. Anthrylaceton (III) dargestellt.
Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome (2004)

The involvement of mucosal mast cells (MMC) in the pathophysiology of irritable bowel syndrome (IBS) is still controversial. We aimed to re-evaluate the role of MMC in visceral hypersensitivity associated with IBS using a rat IBS model that develops the IBS symptom after a subsidence of acetic acid-induced colitis. No significant difference in the number of MMC was observed between normal rat colon and IBS rat colon. (61.7 +/- 2.9/mm(2) in normal vs. 88.7 +/- 13.3/mm(2) in IBS, p > 0.29). However, the degranulation rate of MMC was significantly higher in IBS rat colon (49.5 +/- 2.4% in normal vs. 68.8 +/- 3.4% in IBS, p < 0.05). Pretreatment of a mast cell stabilizer, doxantrazole (5 mg/kg, i.p.), reduced the degranulation rate of MMC and significantly attenuated visceral hypersensitivity to rectal distension in IBS rat, whereas it had no effect on the visceral sensory responses in normal rat. These results suggest that, although the number of MMC is not significantly changed in IBS rat colon, the higher degranulation rate of MMC is responsible for visceral hypersensitivity in this model IBS.
Characteristics of circular loop antennas above a lossless ground plane (1981)

The circuit and radiation characteristics of a circular loop antenna above a lossless ground plane are studied. It is shown that the gain of this antenna is higher than that of a half-wave dipole above the ground plane. A gain as high as 10 dB is obtainable with this simple geometry.
Long-Term Persistence of Human Bone Marrow Stromal Cells Transduced with Factor VIII-Retroviral Vectors and Transient Production of Therapeutic Levels of Human Factor VIII in Nonmyeloablated Immunodeficient Mice (2000)

The potential of using bone marrow (BM)-derived human stromal cells for ex vivo gene therapy of hemophilia A was evaluated. BM stromal cells were transduced with an intron-based Moloney murine leukemia virus (Mo-MuLV) retroviral vector that contained the B domain-deleted human factor VIII (FVIIIdeltaB) cDNA. This FVIII-retroviral vector was pseudotyped with the gibbon ape leukemia virus envelope (GALV-env) to attain higher transduction efficiencies. Using optimized transduction methods, high in vitro FVIII expression levels of 700 to 2500 mU of FVIII/10(6) cells per 24 hr were achieved without selective enrichment of the transduced BM stromal cells. After xenografting of 1.5-3 x 106 engineered BM stromal cells into the spleen of nonobese diabetic severe combined immunodeficient (NOD-SCID) mice, human plasma FVIII levels rose to 13 +/- 4 ng/ml but declined to basal levels by 3 weeks postinjection because of promoter inactivation. About 10% of these stromal cells engrafted in the spleen and persisted for at least 4 months after transplantation in the absence of myeloablative conditioning. No human BM stromal cells could be detected in other organs. These findings indicate that retroviral vector-mediated gene therapy using engineered BM stromal cells may lead to therapeutic levels of FVIII in vivo and that long-term engraftment of human BM stromal cells was achieved in the absence of myeloablative conditioning and without neo-organs. Hence, BM stromal cells may be useful for gene therapy of hemophilia A, provided prolonged expression can be achieved by using alternative promoters.
Poly(<scp>D,L</scp>‐lactic acid‐<i>co</i>‐glycolic acid)‐<i>b</i>‐poly(ethylene glycol)‐<i>b</i>‐poly (<scp>D,L</scp>‐lactic acid‐<i>co</i>‐glycolic acid) triblock copolymer and thermoreversible phase transition in water (2002)

Novel thermoreversible gelation behavior of aqueous solutions of ABA-type triblock copolymers composed of the central polyethylene oxide (PEG) block and two poly(D,L-lactic acid-co-glycolic acid) side blocks was found. Phase transition characteristics, such as critical gel concentration (CGC) and lower and upper critical gel temperature (CGT), are closely related to the molecular structure of the triblock copolymers. The CGC and the lower CGT both increases with increasing PEG/PLGA molecular weight ratio. Increasing the GA content in PLGA block induces a somewhat higher CGC. The copolymer forms micelles with a PLGA loop core and a PEG shell in water. Also grouped micelles are identified seemingly due to the bridging of two micelles sharing two PLGA blocks of a block copolymer chain. As the temperature increases the association of micelles increases, which results in gelation. The ABA-type copolymers exhibit a relatively low CGC (<10%) and low sol-gel transition temperatures compared to BAB-type copolymers. As the temperature increases further gel-sol transition is observed, which would result from the shrinkage of micelles with temperature increase. The hydrodynamic size of the micelles is monitored by dynamic laser scattering, and a possible gelation mechanism was suggested.
Temporal relational data model (1997)

This paper incorporates a temporal dimension to nested relations. It combines research in temporal databases and nested relations for managing the temporal data in nontraditional database applications. A temporal data value is represented as a temporal atom; a temporal atom consists of two parts: a temporal set and a value. The temporal atom asserts that the value is valid over the time duration represented by its temporal set. The data model allows relations with arbitrary levels of nesting and can represent the histories of objects and their relationships. Temporal relational algebra and calculus languages are formulated and their equivalence is proved. Temporal relational algebra includes operations to manipulate temporal data and to restructure nested temporal relations. Additionally, we define operations to generate a power set of a relation, a set membership test, and a set inclusion test, which are all derived from the other operations of temporal relational algebra. To obtain a concise representation of temporal data (temporal reduction), collapsed versions of the set-theoretic operations are defined. Procedures to express collapsed operations by the regular operations of temporal relational algebra are included. The paper also develops procedures to completely flatten a nested temporal relation into an equivalent 1 NF relation and back to its original form, thus providing a basis for the semantics of the collapsed operations by the traditional operations on 1 NF relations.
Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo (2009)

Cre/LoxP recombination is the gold standard for conditional gene regulation in mice in vivo. However, promoters driving the expression of Cre recombinase are often active in a wide range of cell types and therefore unsuited to target more specific subsets of cells. To overcome this limitation, we designed inactive "split-Cre" fragments that regain Cre activity when overlapping co-expression is controlled by two different promoters. Using transgenic mice and virus-mediated expression of split-Cre, we show that efficient reporter gene activation is achieved in vivo. In the brain of transgenic mice, we genetically defined a subgroup of glial progenitor cells in which the Plp1- and the Gfap-promoter are simultaneously active, giving rise to both astrocytes and NG2-positive glia. Similarly, a subset of interneurons was labelled after viral transfection using Gad67- and Cck1 promoters to express split-Cre. Thus, split-Cre mediated genomic recombination constitutes a powerful spatial and temporal coincidence detector for in vivo targeting.
Using Force Sensitive Resistors to Evaluate Hand Tool Grip Design (1992)

The pressure distribution of the hand is an important element in evaluating hand tool grip. For measuring the pressure distributions of the hand, a portable sensor glove was developed using force sensitive resistors of the conductive polymer type. Pressure distributions of three hand tools for three male subjects were measured. The pressure distribution pattern varied significantly between type of grip, location, and subjective exertion level. Factor analysis was used to identify the relative importance of each area of the hand during grip action. The results showed that there is an ‘active' area involved in gripping the hand tool and a ‘control’ area which supports the hand motion for the given task. Studies about hand tool pressure distributions will provide an important guideline in designing a new tool or improving an existing one. A microcomputer based system is being developed that records data on grip pressure distribution throughout a tool grip task. The digitized data then will be analyzed both graphically and statistically and will be used to evaluate various hand tools for industrial settings.
In vitro analysis of the role of glucose oxidase from Talaromyces flavus in biocontrol of the plant pathogen Verticillium dahliae (1996)

Culture filtrates from Talaromyces flavus grown on glucose contained high levels of glucose oxidase activity, while culture filtrates from T. flavus grown on xylan contained negligible glucose oxidase activity. Culture filtrates from T-flavus grown on both media contained complex protein profiles. However, only culture filtrates from T. flavus grown on glucose inhibited germination of microsclerotia of Verticillium dahliae in in vitro inhibition assays. A polyclonal antiserum preparation, pABGO-1, raised against purified glucose oxidase from T. flavus was highly specific for glucose oxidase. Only one protein band in culture filtrates (from glucose medium), migrating at 71 kDa, was detected in Western blots (immunoblots) with this antiserum. This band comigrated with purified glucose oxidase. No bands were detected in culture filtrates from the xylan medium. Glucose oxidase was removed via immunoprecipitation from culture filtrates of T. flavus grown in glucose medium, resulting in filtrates which no longer inhibited in vitro microsclerotial germination. When glucose oxidase-depleted filtrates were amended with purified glucose oxidase from T. flavus, the ability to kill microsclerotia in vitro was restored to original levels. We conclude that glucose oxidase is the only protein in culture filtrates of T. flavus responsible for inhibition of germination of microsclerotia of V. dahliae.
Wayside War Grave in Brittany (1951)

Journal Article Wayside War Grave in Brittany Get access Margaret Willy Margaret Willy Search for other works by this author on: Oxford Academic Google Scholar English: Journal of the English Association, Volume 8, Issue 48, Autumn 1951, Pages 280-a–280, https://doi.org/10.1093/english/8.48.280-a Published: 01 October 1951
Expression and tumorigenicity of the Epstein-Barr virus BARF1 gene in human Louckes B-lymphocyte cell line. (1994)

We previously showed that the Epstein-Barr virus, which encodes the BARF1 gene, could transform rodent fibroblasts. In this work, the expression of the BARF1 gene was studied in the human Louckes B-lymphocyte cell line. Introduction of the BARF1 open reading frame under the control of the Mo-MuLV LTR promotor into nontumorigenic Louckes lymphoid cells led to the activation of the c-myc protooncogene and increased expression of the B-cell surface proteins, the transferrin receptor, CD21, and CD23. BARF1-expressing cells induced a diffuse lymphoma-like tumor in newborn rats treated with anti-thymocyte serum that was, however, transient and regressed after 3-4 weeks as the immune system recovered. The tumor induction was similar to that observed with lymphoid cell lines in vitro generated by infection with the B95-8 virus strain, in which lytic antigens are expressed at low levels. After long-term culture, Louckes cell clones lost expression of the BARF1 gene and were unable to induce tumors.
New photostimulable phosphor materials for digital radiography (2000)

Intense photostimulated luminescence (PSL) is observed, for the first time, in X-ray-irradiated KBr:Eu and KCl/sub x/Br/sub 1-x/Eu phosphor ceramics. The peak in the stimulation spectrum for the PSL red-shifted with increasing the Br content of KCl/sub x/Br/sub 1-x/:Eu phosphor ceramics. The intensity of the PSL with a peak of about 420 nm increases linearly with increasing X-ray irradiation dose over a wide range. The KBr:Eu phosphor exhibites better fading characteristics at room temperature (RT) in the dark, comparing with that of BaFBr:Eu phosphor. The mechanism of fading of the PSL is also discussed.