gebhart/patrepeval huggingface dataset:
Expression of an acidic isoform of calponin in rat brain: western blots on one- or two-dimensional gels and immunolocalization in cultured cells (1995)

Calponin, an actin- and Ca(2+)-calmodulin-binding protein characterized as an inhibitory factor of the smooth-muscle actomyosin activity, has also been shown to be present in some non-muscle cells. However, there is a controversy as to whether calponin is present or not in brain. Several laboratories indicate that this protein is absent in chicken or bovine brains, while Applegate et al. [Applegate, Feng, Green and Taubman (1994) J. Biol. Chem. 269, 10683-10690] have recently reported the identification of an mRNA specific for a 36 kDa non-muscle calponin analogue in homogenates of rat brains. For the first time we demonstrate, by Western blots and in situ immunofluorescence localization using monoclonal as well as affinity-purified polyclonal antibody to gizzard calponin, that a 36-37 kDa and a 35-36 kDa calponin-like proteins are expressed respectively in pig and rat brains and in rat cerebellar cultured cells. The acidic pI (5.2-5.4) of the rat brain protein revealed by isoelectric focusing is in good agreement with that of the protein coded for by the calponin isoform mRNA described by Applegate et al. and is different from that of the protein from chicken gizzard (pI 9.9). Brain calponin-like protein is different from two other Ca(2+)-calmodulin-binding proteins previously identified in brain, namely caldesmon and adducin, and from tropomyosin.
Biomechanical Properties of <i>In Vivo</i> Human Skin From Dynamic Optical Coherence Elastography (2009)

Dynamic optical coherence elastography is used to determine in vivo skin biomechanical properties based on mechanical surface wave propagation. Quantitative Young's moduli are measured on human skin from different sites, orientations, and frequencies. Skin thicknesses, including measurements from different layers, are also measured simultaneously. Experimental results show significant differences among measurements from different skin sites, between directions parallel and orthogonal to Langer's lines, and under different skin hydration states. Results also suggest surface waves with different driving frequencies represent skin biomechanical properties from different layers in depth. With features such as micrometer-scale resolution, noninvasive imaging, and real-time processing from the optical coherence tomography technology, this optical measurement technique has great potential for measuring skin biomechanical properties in dermatology.
Frequency of <i>BRAF</i> T1796A mutation in papillary thyroid carcinoma relates to age of patient at diagnosis and not to radiation exposure (2005)

In this study, the frequency of BRAF mutation was investigated in a series of 67 cases of papillary thyroid cancer (PTC) in patients from Ukraine. Thirty-two patients were aged 30 years or older at the time of diagnosis and 35 were under 16. Tumour was microdissected from paraffin wax-embedded sections, DNA extracted, and the presence of the BRAF T1796A mutation demonstrated by two different methods: PCR followed by restriction enzyme digestion or primer extension assay and detection using MALDI-TOF mass spectrometry. Eighteen (58%) of the adult cases, but only one of the 35 cases aged less than 16 harboured a BRAF T1796A mutation. There was complete agreement between the two methods used, suggesting that the MALDI-TOF assay is a robust alternative to conventional mutation analysis. RET rearrangement was also examined in the young cohort. The overall frequency of RET rearrangement was 45.7%. Eight of the younger group of patients were born after 1 December 1986 and were therefore not exposed to radioiodine in fallout from Chernobyl. None of the PTCs from these eight patients were positive for BRAF mutation. The frequency of RET rearrangement was 44% in the 27 cases exposed to radiation and 50% in the eight not exposed. These results suggest that the different molecular biological profiles observed are associated with the age of the patient at diagnosis with PTC, rather than being associated with radiation exposure.
Characterization of a quarter torus filter for vacuum arc ion flux transmission (2004)

Measurements on the ion flux transmission in a magnetically filtered d-c vacuum arc are presented. The device includes a metallic plasma-generating chamber with water-cooled electrodes coupled to a substrate chamber through a quarter-torus magnetic filter. It is employed a Copper cathode (6 cm in diameter) with a Copper annular anode. The filter consists in a steel cylinder 500 mm length and 100 mm inner diameter with 90o of bending angle and corrugated lateral walls, surrounded by a magnetic field generating coil. The arc is operated at a current level of 100 A, and the intensity of the filtering magnetic field was in the range 0¡200 G (measured at the knee of the filter). This magnetic field is enough high so as to magnetize the electrons but not the ions. The discharge is ignited by bringing (and later removing) a tungsten striker into contact with the cathode. The arc voltage drop, the floating potentials of the filter and the plasma, and the ion current collected by probes located at different positions as functions of the magnetic field intensity are reported and compared with measurements presented in the literature with other similar devices.
Stimuli-Responsive Programmed Specific Targeting in Nanomedicine (2016)

Both passive targeting and actively enhanced cellular internalization play significant roles in tumor-targeted therapy. Programmed specific targeting, as a novel targeting strategy that exploits stimuli-responsive structures, expects that nanocarriers show high stability during blood circulation for efficient passive targeting, then respond to tumor internal or external stimuli and transform into more cell-interactive forms upon arrival at the tumor tissue for enhanced cellular internalization. In this Perspective, we introduce recent advances in the design and development of stimuli-responsive programmed specific targeting nanomedicines, which are based on switchable surface charge, activatable targeting molecules, and variable coatings, to combine the advantages of passive targeting and actively enhanced cellular internalization.
Thermally induced segregation of SnO2 nanoclusters in Sn-doped silica glasses from oversaturated Sn-doped silica xerogels (2001)

Silica glasses with substitutional Sn-doping or with dispersion of SnO2 nanoclusters were prepared by the sol–gel method. The occurrence of SnO2 clustering was investigated by optical absorption, Raman scattering and electron paramagnetic resonance spectroscopies, varying both the tin concentration and the densification temperature of Sn-doped silica xerogels. The 3.7 eV absorption edge of SnO2 and the A1g Raman mode at 630 cm−1 of the SnO2 phonon spectrum were used to assess the presence of SnO2 nanoclusters; the yield of X-ray induced paramagnetic E′ Sn centres was monitored to probe the variation in the amount of substitutional Sn centres. The results indicate that the clustering of SnO2 becomes effective for Sn concentrations ≥ 0.8 mol% and it is competitive with the substitutional doping in xerogels densified at T ≥ 900 °C. No evidence of pre-existent clustering was observed in xerogels densified at lower temperatures in the investigated doping range.
Immunological Cells and Functions in Gaucher Disease (2013)

The macrophage (MΦ) has been the focus of causality, research, and therapy of Gaucher disease, but recent evidence casts doubt its solitary role in the disease pathogenesis. The excess of glucosylceramide (GC) in such cells accounts for some of the disease manifestations. Evidence of increased expression of C-C and C-X-C chemokines (i.e., CCL2,CXCL1, CXCL8) in Gaucher disease could be critical for monocyte transformation to inflammatory subsets of macrophages and dendritic cells (DC) as well as neutrophil (PMNs) recruitment to visceral organs. These immune responses could be essential for activation of T- and B-cell subsets, and the induction of numerous cytokines and chemokines that participate in the initiation and propagation of the molecular pathogenesis of Gaucher disease. The association of Gaucher disease with a variety of cellular and humoral immune responses is reviewed here to provide a potential foundation for expanding the complex pathophysiology of Gaucher disease.
Service Quality: A Measure of Information Systems Effectiveness (1995)

The IS function now includes a significant service component. However, commonly used measures of IS effectiveness focus on the products, rather than the services, of the IS function. Thus, there is the danger that IS researchers will mismeasure IS effectiveness if they do not include in their assessment package a measure of IS service quality. SERVQUAL, an instrument developed by the marketing area, is offered as a possible measure of IS service quality. SERVQUAL measures service dimensions of tangibles, reliability, responsiveness, assurance, and empathy. The suitability of SERVQUAL was assessed in three different types of organizations in three countries. After examination of content validity, reliability, convergent validity, nomological validity, and discriminant validity, the study concludes that SERVQUAL is an appropriate instrument for researchers seeking a measure of IS service quality.
CHARACTERIZATION OF DYE-SENSITIZED PHOTOOXIDATION OF MUSHROOM TYROSINASE (1989)

Dye-sensitized photooxidation was investigated as a nonthermal means to inactivate food quality-related enzymes, using mushroom tyrosinase as a model. Illumination of tyrosinase in the presence of either rose bengal or riboflavin resulted in an apparent first-order destruction of enzyme activity. Both dye and light were required, and photoinactivation was favored by increasing levels of dissolved oxygen. The rose bengal-sensitized photooxidation was generally more rapid than that caused by riboflavin (at 0.01% dye), with ki values ranging from 0.74 to 1.66 h−1 and 0.25 to 1.23 h−1, respectively. First-order rate constants for photoinactivation decreased with decreasing temperatures (Ea was 8.4 to 12.2 kcal mol−1 between 20° and 50°C), increasing protein concentration (0.175 to 1.40 mg−1 ml), increasing sodium phosphate buffer concentration (10 to 200 mM) and increasing ionic strength (0.02 to 0.20). The dependence of enzyme photoinactivation rates on pH (between 6.0 and 9.0) resembled a titration curve with a pK of 7.5, and maximal rates were observed at pH 8.0–9.0.
Parameters controlling nitric oxide emissions from gas turbine combustors. (1973)

Nitric oxide forms in the primary zone of gas turbine combustors where the burnt gas composition is close to stoichiometric and gas temperatures are highest. It has been found that combustor air inlet conditions, mean primary zone fuel-air ratio, residence time, and the uniformity of the primary zone are the most important variables affecting nitric oxide emissions. Relatively simple model of the flow in a gas turbine combustor, coupled with a rate equation for nitric oxide formation via the Zeldovich mechanism are shown to correlate the variation in measured NOx emissions. Data from a number of different combustor concepts are analyzed and shown to be in reasonable agreement with predictions. The NOx formulation model is used to assess the extent to which an advanced combustor concept, the NASA swirl can, has produced a lean well-mixed primary zone generally believed to be the best low NOx emissions burner type.
Intracapsular Prostatic Omentalization: A New Technique for Management of Prostatic Abscesses in Dogs (1995)

Twenty dogs with abscessation of the prostate gland were treated by celiotomy, drainage, and digital exploration of the gland through bilateral capsulectomy wounds. Omentum was introduced through the capsulectomy wounds and packed into the abscess cavities around the prostatic urethra. Most dogs were discharged from the hospital within 48 hours of the surgery. Long‐term resolution of the problem (no recurrence within 12 months) was achieved in 19 dogs. One dog had recurrent abscessation that was managed by the placement of dependant Penrose drains. Temporary tube cystostomy was performed postoperatively in one dog for the management of transient dysuria. One dog with septic peritonitis resulting from preoperative abscess rupture was managed by concurrent open peritoneal drainage. Intracapsular prostatic omentalization was a simple and effective means of managing prostatic abscessation with minimal requirement for postoperative hospitalization of the patient. The frequency of serious complications, including postoperative urinary incontinence, was low.
Development of a Novel Ice Sensing and Active De-icing Method for Wind Turbines (2012)

The best wind resources in terms of high wind speeds are often located in cold wet regions where atmospheric icing is likely on the blades of wind turbines. Icing can cause several problems for the operation of wind turbines in cold climates such as (1) reducing the energy production by lowering the aerodynamic eciency of the rotor, (2) reducing the life expectancy of the turbine by increasing the fatigue on the structure, and (3) representing a risk for people and properties around the turbine. Current de-icing systems for wind turbines largely originate from the aircraft industry, and only a very few tests have been performed so far to optimize their design for wind turbines. Thus, much work remains to be done to develop more satisfactory methods for preventing ice and de-icing for wind turbines. We aim to develop new techniques of rapid and accurate ice detection and de-icing to reduce both the operating cost and energy consumption of anti-icing and de-icing. In this paper, we begin with a tutorial and literature survey on de-icing of wind turbines. We then discuss the advantages and disadvantages of dierent methods of active de-icing systems on wind turbines, calculate heat ux requirements of de-icing systems (blade thermodynamics) and relate our results to wind turbine energy protability. Finally we will demonstrate an accurate and direct on-the-blade ice detection technique utilizing optical frequency-domain reectometry(OFDR ) to yield (a) ice thickness measurement within micron accuracy as well as (b)an identication of the type of ice present.
UV epoxy bonding for enhanced SAW transmission and microscale acoustofluidic integration (2012)

Surface acoustic waves (SAWs) are appealing as a means to manipulate fluids within lab-on-a-chip systems. However, current acoustofluidic devices almost universally rely on elastomeric materials, especially PDMS, that are inherently ill-suited for conveyance of elastic energy due to their strong attenuation properties. Here, we explore the use of a low-viscosity UV epoxy resin for room temperature bonding of lithium niobate (LiNbO(3)), the most widely used anisotropic piezoelectric substrate used in the generation of SAWs, to standard micromachined superstrates such as Pyrex® and silicon. The bonding methodology is straightforward and allows for reliable production of sub-micron bonds that are capable of enduring the high surface strains and accelerations needed for conveyance of SAWs. Devices prepared with this approach display as much as two orders of magnitude, or 20 dB, improvement in SAW transmission compared to those fabricated using the standard PDMS elastomer. This enhancement enables a broad range of applications in acoustofluidics that are consistent with the low power requirements of portable battery-driven circuits and the development of genuinely portable lab-on-a-chip devices. The method is exemplified in the fabrication of a closed-loop bidirectional SAW pumping concept with applications in micro-scale flow control, and represents the first demonstration of closed channel SAW pumping in a bonded glass/LiNbO(3) device.
CW Yb,Tm:LiYF4 Upconversion Laser at 650 nm, 800 nm, and 1500 nm (1995)

Continious wave laser emission at visible and IR-transitions in Yb,Tm:LiYF4 have been obtained at 300 K by pumping Yb at 960 nm and using upconversion energy transfer mechanisms.
Nanofibrous Scaffolds and their Biological Effects (2003)

Abstract The sections in this article are Overview Introduction Methods of Formation Electrospinning Self‐assembly Phase Separation Nanofibrous Composite Scaffolds Inorganic Composites Surface Modification Factor Delivery Scaffolds Biological Effects of Nanofibers Attachment Proliferation Differentiation Migration Tissue Formation Connective Tissue Ligaments Cartilage Bone Neural Tissue Cardiovascular Tissue Cardiac Muscle Blood Vessel Liver Tissue Conclusion
Review article: the pharmacology of rabeprazole (1999)

Rabeprazole sodium is a new substituted benzimidazole proton pump inhibitor with several differences compared with existing proton pump inhibitors. In vitro and animal studies have demonstrated that rabeprazole is a more potent inhibitor of H+,K(+)-ATPase and acid secretion than omeprazole, and is a more rapid inhibitor of proton pumps than omeprazole, lansoprazole, or pantoprazole. This probably reflects rabeprazole's faster activation in the parietal cell canaliculus. In human studies, once-daily doses of 5-40 mg of rabeprazole inhibit gastric acid secretion in a dose-dependent fashion. A once-daily dose of 20 mg has consistently achieved profound decreases in 24-h intragastric acidity in single and repeat dosing studies, in healthy volunteers and patients with either peptic ulcer disease or gastro-oesophageal reflux disease. Significantly greater decreases in intragastric acidity are achieved on day 1 of dosing with rabeprazole 20 mg than with omeprazole 20 mg. As with other proton pump inhibitors, rabeprazole has in vitro antibacterial activity against Helicobacter pylori, with greater activity against this organism than either lansoprazole or omeprazole. In addition to inhibiting bacterial urease activity, rabeprazole binds to several molecules on H. pylori. Clinical trials are needed to assess the clinical importance of these findings, as well as to assess whether the potential advantages of rabeprazole result in clinical benefit for patients with acid-related diseases.
Chemical Vapor Cleaning of 6H‐SiC Surfaces (1999)

The techniques (temperature range of study) of in situ thermal desorption (500‐1100°C) and chemical vapor cleaning (CVC) via exposure to and/or (750‐1100°C) have been investigated for preparing surfaces suitable for epitaxial growth of SiC and III‐nitride films, and are compared with regard to surface purity, stoichiometry, and structural order. Oxide removal below the detection limits of Auger electron spectroscopy was achieved for all orientations via annealing in at 850‐900°C or ≈200° lower than necessary by thermal desorption. No non‐SiC carbon was detected on the surface by X‐ray photoelectron spectroscopy. An approximately one‐tenth of a monolayer of oxygen coverage and significant quantities of non‐SiC carbon were detected for all 6H‐SiC surfaces prepared by thermal desorption. In contrast to the predominantly non‐SiC carbon‐rich surfaces prepared by thermal desorption, the stoichiometry of the SiC surfaces prepared by CVC could be manipulated from Si‐rich to C‐rich without non‐SiC carbon formation by either extending the exposures or by following with exposure. The latter surfaces also had lower concentrations of both oxygen and non‐SiC carbon and increased surface order. © 1999 The Electrochemical Society. All rights reserved.
Taking the Skeletons Out of the Closets: A Simple and Efficient Topology Discovery Scheme for Large Ethernet LANs (2006)

We propose a simple and efficient algorithmic solution for discovering the physical topology of large, heterogeneous Ethernet LANs that may include multiple subnets as well as uncooperative network elements, like hubs. Our scheme utilizes only generic MIB information and does not require any hardware or software modification of the underlying network elements. By rigorous analysis, we prove that our method correctly infers the network topology and has low communication and computational overheads. Our simulation results show that the scheme successfully infers the complete topology in the vast majority of the cases, including many instances that cannot be inferred by other methods. Finally, our proof-of-concept implementation demonstrates the practicality of the proposed scheme for network management.
Effect of light source and specimen thickness on the surface hardness of resin composite. (2002)

The power density delivered by each light was: PAC over 1999 mW/cm2, QTH+S 588 mW/cm2, and QTH+T 844 mW/cm2. Both the thickness of the composite and the light source had a significant effect on the hardness. Overall, using the QTH+T produced the highest hardness values and the PAC light the lowest values. For all 2-mm thick specimens, irrespective of the light source, the hardness values at the bottom were not significantly different from their top hardness values measured at the same time. For the 3-mm thick specimens using the QTH+T, the hardness values at the bottom of both composites were not significantly different from their top hardness values at all time intervals. For the 3-mm thick specimens using the QTH+S, the bottom hardness values were not significantly different from those at the top for P60 only, and only at 24 hours and 7 days, but not when measured immediately. For all the 4 and 5-mm thick specimens, the hardness values at the bottom were all significantly different from those at the top measured at the same time interval.
Ligand-independent assembly of recombinant human CD1 by using oxidative refolding chromatography (2001)

CD1 is an MHC class I-like antigen-presenting molecule consisting of a heavy chain and β 2 -microglobulin light chain. The in vitro refolding of synthetic MHC class I molecules has always required the presence of ligand. We report here the use of a folding method using an immobilized chaperone fragment, a protein disulphide isomerase, and a peptidyl-prolyl cis - trans isomerase (oxidative refolding chromatography) for the fast and efficient assembly of ligand-free and ligand-associated CD1a and CD1b, starting with material synthesized in Escherichia coli . The results suggest that “empty” MHC class I-like molecules can assemble and remain stable at physiological temperatures in the absence of ligand. The use of oxidative refolding chromatography thus is extended to encompass complex multisubunit proteins and specifically to members of the extensive, functionally diverse and important immunoglobulin supergene family of proteins, including those for which a ligand has yet to be identified.
Rhodopsin mutations in autosomal dominant retinitis pigmentosa (1993)

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form (adRP), which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. So far, 41 single-base-pair (bp) substitutions, one two-bp substitution, and four deletions ranging from 3 to 42 bp have been identified in this gene. These mutations do not appear to be significantly clustered in a specific part of the protein, but occur in all three major domains, namely the intradiscal, transmembrane, and cytoplasmic regions. Different mutations appear to cause differences in the severity of the disease, though there is considerable variability in severity even within the same family, at least in certain of these mutations. Identification of all the mutations involved in rhodopsin-RP should allow accurate and early detection of affected individuals, informed genetic counselling, as well as furthering our knowledge of the disease process involved.