gebhart/patrepeval huggingface dataset:
Synthesis and Characterization of Chimeric Silkworm Silk (2003)

A synthetic gene encoding a chimeric silklike protein was constructed that combined a polyalanine encoding region (Ala)(18), a sequence slightly longer than the (Ala)(12-13) found in the silk fibroin from the wild silkworm Samia cynthia ricini, and a sequence encoding GVGAGYGAGAGYGVGAGYGAGVGYGAGAGY, found in the silk fibroin from the silkworm Bombyx mori. A tetramer of the chimeric repeat sequence encoding a approximately 29 kDa protein was expressed as a fusion protein in Escherichia coli. In comparison to S. c. ricini silk, the chimeric protein demonstrated improved solubility because it could be dissolved in 8 M urea. The purified protein assumed an alpha-helical structure based on solid-state (13)C CP/MAS NMR and was less prone to conformational transition to a beta-sheet, unlike native silk proteins from S. c. ricini. Model peptides representing the crystalline region of S. c. ricini silk fibroin, (Ala)(12) and (Ala)(18), formed beta-sheet structures. Therefore, the solubility and structural transitions of the chimeric protein were significantly altered through the formation of this chimeric silk. This experimental strategy to the study of silk structure and function can be used to develop an improved understanding of the contributions of protein domains in repetitive silkworm and spider silk sequences to structure development and structural transitions.
Labeling board based on boundary tracking (1995)

Abstract This paper reports on hardware implementation of a labeling algorithm using a combination of raster scanning and boundary tracking. For the prototype board, a new method is employed for generating addresses of image memory so that boundary tracking is efficient. Since boundary tracking is used, there is no need for working memory for storage of initial labels. Hence the circuit becomes compact, and the labeling can be performed without restricting the number of labels. Furthermore, it can also count the number of holes when labeling and it can set the minimum or maximum boundary length of the region to be labeled. This board is constructed using only general purpose ICs; for a 512 × 512 image, the processing time is approximately 50 ms.
Intelligent Control and Integration Software for Flexible Manufacturing Cells (2007)

Flexible manufacturing cells (FMC) are capable of producing a broad variety of products and changing their characteristics quickly and frequently. This flexibility provides for more efficient use of resources but makes the control of these systems more difficult. This paper presents several software applications developed for industrial robots and computer numerical control (CNC) machines. The objective of this software is to allow these equipments to be integrated, in an easy and efficient way, in modern FMC. For the industrial robots, we developed the "winRS232ROBOTcontrol" and "winEthernetROBOTcontrol" software. For the CNC machines, the software that was developed was "winMILLcontrol" for the mill and "winTURNcontrol" for the lathe. With this software, industrial robots and CNC machines can be integrated in modern FMC, in an easy and efficient way. An FMC was developed, with industrial characteristics, with the objective of showing the potentialities of the developed software. Thus, the proposed software in this paper was tested and validated in the developed FMC, and the experimental results show the viability and success of this software in the coordination of the different sectors in the FMC and in the improvement of their performance
Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction (2014)

Herein, we describe a novel cloning strategy for PCR-amplified DNA which employs the type IIs restriction endonuclease BsaI to create a linearized vector with four base-long 5'-overhangs, and T4 DNA polymerase treatment of the insert in presence of a single dNTP to create vector-compatible four base-long overhangs. Notably, the insert preparation does not require any restriction enzyme treatment. The BsaI sites in the vector are oriented in such a manner that upon digestion with BsaI, a stuffer sequence along with both BsaI recognition sequences is removed. The sequence of the four base-long overhangs produced by BsaI cleavage were designed to be non-palindromic, non-compatible to each other. Therefore, only ligation of an insert carrying compatible ends allows directional cloning of the insert to the vector to generate a recombinant without recreating the BsaI sites. We also developed rapid protocols for insert preparation and cloning, by which the entire process from PCR to transformation can be completed in 6-8 h and DNA fragments ranging in size from 200 to 2200 bp can be cloned with equal efficiencies. One protocol uses a single tube for insert preparation if amplification is performed using polymerases with low 3'-exonuclease activity. The other protocol is compatible with any thermostable polymerase, including those with high 3'-exonuclease activity, and does not significantly increase the time required for cloning. The suitability of this method for high-throughput cloning was demonstrated by cloning batches of 24 PCR products with nearly 100% efficiency. The cloning strategy is also suitable for high efficiency cloning and was used to construct large libraries comprising more than 108 clones/µg vector. Additionally, based on this strategy, a variety of vectors were constructed for the expression of proteins in E. coli, enabling large number of different clones to be rapidly generated.
A Tunable Squirrel-Cage Magnetron-The Donutron (1947)

The donutron is an all-metal squirrel-cage magnetron. It is a multisegment magnetron with a single resonant structure. It is tuned by the relative axial displacement of alternate anode segments, through flexure of one wall of the cavity in which the anode structure is supported. During the testing of some sixty models, the operating efficiency and the output power have been increased to 40 to 50 per cent and around 50 watts, respectively (6- to 12-centimeter range). The best model to date tunes over a 1.5 to 1 ratio with power flat to 3 decibels. A single value of voltage and magnetic field is adequate for the entire tuning range. Of the various modes of operation, two are important, a long-wave tunable cavity mode and a shortwave resonant re-entrant-line mode. The former can be entirely suppressed and the latter enhanced by a special phase-reversing anode. In the line mode the highest output powers have been observed. In cold tests, modes around 4 centimeters as well as indication of even shorter resonance wavelengths have been found. A wide range of design parameters has been studied. Rieke diagrams have been taken for the best tubes.
Non-alcoholic fatty liver and the gut microbiota (2016)

Intestinal host-microbiome interactions play diverse roles in the pathogenesis and progression of NAFLD and NASH. Elucidation of the mechanisms driving these microbial effects on the pathogenesis of NAFLD and NASH may enable to identify new diagnostic and therapeutic targets of these common metabolic liver diseases. This article is part of a special issue on microbiota.
B1.3 - Printed Piezoresistive Strain Sensors for Monitoring of Light-Weight Structures (2011)

In this paper we present the design and test of printed strain sensors, which can be integrated in lightweight structures for monitoring purposes. We focus on composite structures consisting of a metal substrate and insulating and conductive ink layers for sensing normal strain at the surface. Both, inkjet and screen printing technology are used to realize resistive topologies. In a first step, we analyze electrical properties of functional inks: electrical impedance and breakdown electrical field strength in case of insulation inks, resistance in case of conducting inks. Silver and PEDOT:PSS based suspensions are printed as sensing layer. To determine the resistance change due to plastic deformation of the metal substrate, tensile tests are performed up to 30% strain and subsequent resistance change is measured. In a second step, the sensing effect of printed conductive structures is investigated. Resistive sensing topologies are designed for detecting longitudinal and transversal normal strain. Meander structures, which form single resistors as well as bridge configurations, are printed on test specimens and analyzed in a four-point bending set up. Performing loading and unloading cycles, gauge factor, cross sensitivity, nonlinearity and hysteresis error of the sensors are measured.
CMR manganites: physics, thin films and devices (2003)

The manganese oxides of general formula RE1−xMxMnO3 (RE = rare earth, M = Ca, Sr, Ba, Pb) have remarkable interrelated structural, magnetic and transport properties induced by the mixed valence (3+–4+) of the Mn ions. In particular, they exhibit very large negative magnetoresistance, called colossal magnetoresistance (CMR), in the vicinity of metal–insulator transition for certain compositions. In this review paper, we summarize the most important features of the physics of the CMR manganites. The growth techniques for manganese oxide thin films, which are the basic material for potential applications, are reviewed and their structure and morphology examined in relation to growth parameters. The effects of epitaxial strains on the physical properties are discussed. Early works on superlattices and devices are presented.
Correcting atmospheric masking to retrieve the spectral albedo of land surfaces from satellite measurements (1995)

Abstract A radiative transfer approach to the problem of atmospheric correction of satellite images in the solar spectral range is presented which includes all multiple scattering processes without any approximation. The numerical solution is accepted as satisfying, if the numerical accuracy is better than I per cent. This means that the accuracy of the atmospheric correction depends almost exclusively on the quality of the auxiliary data on the atmospheric state and the surface reflection indicatrix. Byextensivemodel calculations these parameter driven error bounds have been quantified. Thus the calculation results in a corrected albedo image with specified error bounds. This seems to be the first algorithm available for atmospheric correction of real imagery data which relies on a numerical exact treatment of multiple scattering. The program EXACT (EXact Atmospheric Correction Technique) has so far been used with Landsat Thematic Mapper (TM), NOAA AVHRR (National Oceanic and Atmospheric Administration Advanced Very High Resolution Radiometer) and also with airborne Daedalus ATM images. The algorithm has been validated by comparison of satellite data to ground measurements and between different sensors. Errors of the derived albedos were found to remain below 0·01 for visible and near-infrared sensor channels of this set of radiometers.
Deploying Analytics with the Portable Format for Analytics (PFA) (2016)

We introduce a new language for deploying analytic models into products, services and operational systems called the Portable Format for Analytics (PFA). PFA is an example of what is sometimes called a model interchange format, a language for describing analytic models that is independent of specific tools, applications or systems. Model interchange formats allow one application (the model producer) to export models and another application (the model consumer or scoring engine) to import models. The core idea behind PFA is to support the safe execution of statistical functions, mathematical functions, and machine learning algorithms and their compositions within a safe execution environment. With this approach, the common analytic models used in data science can be implemented, as well as the data transformations and data aggregations required for pre- and post-processing data. PFA compliant scoring engines can be extended by adding new user defined functions described in PFA. We describe the design of PFA. A Data Mining Group (DMG) Working Group is developing the PFA standard. The current version is 0.8.1 and contains many of the commonly used statistical and machine learning models, including regression, clustering, support vector machines, neural networks, etc. We also describe two implementations of Hadrian, one in Scala and one in Python. We discuss four case studies that use PFA and Hadrian to specify analytic models, including two that are deployed in operations at client sites.
Carrier frequency estimation in satellite communications (2003)

For the estimation of the carrier frequency shift in satellite communications using QPSK modulation, two methods are usually considered to eliminate the data modulation: multiplying the phase of the received samples by 4 and keeping their amplitude or setting it to unity. A study of the operating threshold of the carrier frequency estimation is carried out in both cases in the presence of additive white Gaussian noise. A technique based on the expectation of a noisy sample in each case is proposed and verified by simulation, with the help of an efficient algorithm for the frequency estimation. The application envisaged is the estimation of the carrier frequency in a satellite receiver in burst mode transmission.
Novel rhodium(I)-porphyrin complexes and organorhodium(III)-porphyrin complexes. IV (1975)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNovel rhodium(I)-porphyrin complexes and organorhodium(III)-porphyrin complexes. IVHisanobu Ogoshi, Junichiro Setsune, Takashi Omura, and Zenichi YoshidaCite this: J. Am. Chem. Soc. 1975, 97, 22, 6461–6466Publication Date (Print):October 1, 1975Publication History Published online1 May 2002Published inissue 1 October 1975https://pubs.acs.org/doi/10.1021/ja00855a027https://doi.org/10.1021/ja00855a027research-articleACS PublicationsRequest reuse permissionsArticle Views1023Altmetric-Citations122LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characterization and Angiogenic Potential of Human Neonatal and Infant Thymus Mesenchymal Stromal Cells (2015)

Abstract Resident mesenchymal stromal cells (MSCs) are involved in angiogenesis during thymus regeneration. We have previously shown that MSCs can be isolated from enzymatically digested human neonatal and infant thymus tissue that is normally discarded during pediatric cardiac surgical procedures. In this paper, we demonstrate that thymus MSCs can also be isolated by explant culture of discarded thymus tissue and that these cells share many of the characteristics of bone marrow MSCs. Human neonatal thymus MSCs are clonogenic, demonstrate exponential growth in nearly 30 population doublings, have a characteristic surface marker profile, and express pluripotency genes. Furthermore, thymus MSCs have potent proangiogenic behavior in vitro with sprout formation and angiogenic growth factor production. Thymus MSCs promote neoangiogenesis and cooperate with endothelial cells to form functional human blood vessels in vivo. These characteristics make thymus MSCs a potential candidate for use as an angiogenic cell therapeutic agent and for vascularizing engineered tissues in vitro.
Gas–liquid interfacial plasmas: basic properties and applications to nanomaterial synthesis (2009)

A boundary between plasmas and liquids, which activates physical and chemical reactions, has successfully been generated under a low gas pressure condition by utilizing ionic liquids with unique properties such as fully ionized plasma state, extremely low vapor pressure and high heat capacity. Due to the formation and control of a sheath electric field just above the ionic liquid, the plasma-ion behavior can be manipulated and the effects of the ion irradiation on the ionic liquid are quantitatively revealed. In connection with the plasma-ion irradiation, the secondary electron emission is found to be enhanced by introducing the ionic liquid as a cathode electrode. The control of the plasma-ion irradiation flux and energy to the ionic liquid leads to the creation of various kinds of metal nanoparticles by the reduction in metal chlorides and the realization of efficient synthesis. Furthermore, the metal nanoparticles are synthesized with carbon nanotubes as a template in the ionic liquid using the plasma irradiation method. It is found that the high density, mono-dispersed and isolated metal nanoparticles are synthesized between or inside the carbon nanotubes by controlling the gas–liquid interfaced plasmas.
AAPM Medical Physics Practice Guideline 8.a.: Linear accelerator performance tests (2017)

This guideline will assist the QMP in developing a comprehensive QA program for linacs in the external beam radiation therapy setting. The committee sought to prioritize tests by their implication on quality and patient safety. The QMP is ultimately responsible for implementing appropriate tests. In the spirit of the report from American Association of Physicists in Medicine Task Group 100, individual institutions are encouraged to analyze the risks involved in their own clinical practice and determine which performance tests are relevant in their own radiotherapy clinics.
Characterization of a new, potent, immunopathogenic epitope in S-antigen that elicits T cells expressing V beta 8 and V alpha 2-like genes (1991)

Experimental autoimmune uveoretinitis (EAU) is a predominantly T cell-mediated autoimmune disease induced in susceptible animals by active immunization with human or bovine retinal S-Ag or by passive transfer of activated S-Ag or peptide-specific CD4+ T cells. During the course of studies aimed at the identification of T cell and B cell recognition sites in bovine and human S-Ag, a new potent uveitogenic region, located near the carboxy terminus of the molecule, was identified and characterized. Analysis of several synthetic peptides from this region showed that a 14 amino acid residue peptide, BSAg339-352, was highly uveitogenic when injected with adjuvants into Lewis rats. A uveitogenic T cell line, R737, was raised by in vitro selection of lymphocytes from animals immunized with peptide BSAg333-352. Northern blot analysis of mRNA from the R737 T cell line was positive for the rat homologs of murine V beta 8 and V alpha 2 T cell receptor gene probes. Whereas peptide BSAg339-352 defined the pathogenic site, nonpathogenic, proliferative sites were found in close physical association. This region is immediately adjacent to previously characterized pathogenic and proliferative sites contained in residues BSAg352-364. These results, as well as our previous observations, show S-Ag to be a complex molecule with several highly conserved amino acid sequences that can elicit pathogenic T cells with restricted T cell receptor V gene usage capable of active and passive elicitation of experimental autoimmune uveoretinitis.
Cryptanalysis of a Threshold Proxy Signature with Known Signers (2005)

A scheme of threshold proxy signature with known signers was proposed by Hwang et al. In their scheme, the receiver can identify the proxy signers that actually generated proxy signatures. Tzeng et al. demonstrated that this signature scheme is insecure and proposed an improvement to mend the information leakage. This paper shows that the improved scheme is still insecure under the original signer's forgery attack, i.e. insider attack. Our demonstration of insider attack shows that the original signer can freely choose message and delegation warrant and then produces a signature on them.
Cetyltrimethyl Ammonium Bromide (CTAB) DNA Miniprep for Plant DNA Isolation (2009)

INTRODUCTION The presence of polysaccharides in a DNA preparation can inhibit use of techniques such as polymerase chain reaction (PCR). Cetyltrimethyl ammonium bromide (CTAB) is a surfactant useful for isolation of DNA from tissues containing high amounts of polysaccharides. Under the high-salt conditions used in this protocol, the CTAB binds the polysaccharides, removing them from the solution. When used with Arabidopsis , this procedure yields quite pure DNA that is suitable for thermal asymmetric interlaced PCR (TAIL-PCR).
Isolation of hydrogenotrophic methanogenic archaea from a subtropical paddy field (1999)

A total of 347 blue-autofluorescent colonies on roll tube cultures were transferred individually for isolation in order to examine methanogenic flora in a subtropical paddy field after application of organic matter with three treatments (control soil, straw-amended soil, and cellulose-amended soil). Two hundred and three strains showed a positive reaction for methane production. However, only 36 strains were found to be pure. Among the 36 pure methanogenic isolates, 19 utilized H2-CO2 as sole source for energy and carbon for growth, 17 utilized H2-CO2 and formate, and none utilized methanol and acetate. All 36 isolates were rods distinguishable into three groups by cell morphology: group 1, irregularly crooked long rods; group 2, gently curved long (or very long) rods; and group 3, relatively straight long (or very long) rods. Three isolates, strains 3544-4 and 6544-9 of group 2 and 5544-3 of group 3, were identified as Methanobacterium spp. by partial sequencing analysis of 16S rRNA. The partial 16S rRNA sequence of strain 6544-9 was identical to that of Methanobacterium bryantii. The other three isolates which belonged to group 1, strains 3543-10, 3544-9, and 6132-1, seemed to represent a new species within the genus Methanobacterium or probably Methanobrevibacter. Each of the pure isolates from control and straw-amended soil belonged to group 1. It is suggested that the group 1 methanogens are characterized as a geographically interesting indigenous type in the subtropical paddy field.
Errors in the Parallel Connection of a 100 : 1 Series-Parallel Buildup of Four-Terminal Resistors (1972)

This paper deals with the problem of calculating the parallel resistance of a 100: 1 series-parallel buildup of four-terminal resistors with accuracy as high as possible. A rather complex equivalent circuit has been assumed, taking into account all the causes of significant errors. Then a complete calculation has been carried out for obtaining all the error terms expressed by means of 15 relatively synthetic formulas. The method of solving the problem that has been developed is a new one and allows 1) rather simple calculations even if the network is complex, and 2) an immediate separation of the error terms from the main value (in fact, the result is given directly as the sum of a base value and a set of perturbations). The method is based on applying Cohn's theorem and using certain symmetries that have been put in evidence in the equivalent circuit. The perturbations may be considered either as corrections or as uncertainties. Very simple formulas are given for the means and the standard deviations of the errors, the perturbations being considered as uncertainties, and it being assumed that the causes of error are independent of each other and normally distributed. Anyway, these formulas may conveniently be used for initial investigation on the magnitudes of the errors before applying the more complex formulas that give the exact values of the variations. Finally, as an example, results of computations carried out on a particular buildup box are reported.