gebhart/patrepeval huggingface dataset:
Models of Nociception: Hot‐Plate, Tail‐Flick, and Formalin Tests in Rodents (2007)

Experimental models of pain include tests of response thesholds to high intensity stimuli (acute pain tests) and changes in spontaneous or evoked behavioral responses in animals with peripheral injury or inflammation (persistent pain models). Acute thermal pain is modeled by the hot-plate and tail-flick test, while persistent pain can be modeled by the formalin test. This unit presents protocols for all three of these tests, including preparation of animals (rats or mice), administration of a compound being tested for its analgesic properties and data collection.
Codes for Error Correction in High-Speed Memory Systems—Part I: Correction of Cell Defects in Integrated Memories (1971)

This paper introduces two schemes to correct bit errors caused by defective memory cells in high-speed random-access memory systems. The schemes are addressed to word-organized memories produced by the integrated technologies. One of the two schemes calls for encoding of input information and the other does not. The schemes are simple, economical for the technologies concerned, and exhibit a regularity which makes it possible to fabricate the necessary additional hardware within the same technology.
Factors Influencing Isolation of Islets of Langerhans (1989)

More efficient methods of islet isolation must be developed for islet transplantation to become clinically routine. During collagenase dispersal of human pancreas, an amorphous, viscous, gellike material often develops and entraps large numbers of islets, thereby reducing the yield. When donor human pancreas is minced and treated with collagenase, the gel forms most abundantly if the digestion temperature is less than 35 degrees C and if pH falls below 7.2 +/- 0.2. Gel formation appears to be proportional to warm- or cold-ischemia time and may be related to tissue trauma during collection. Once gel has formed, trapped islets cannot be released by filtration, dilution, DNase, incubation temperature, or pH adjustment. These characteristics suggest that the material is gelatin derived from collagen released enzymatically from pancreatic stroma. We demonstrate that gelation is greatly reduced or eliminated when 1) the incubation medium includes glycerol--a common gelatin solvent--at 5% (vol/vol), 2) the minced tissue-to-total incubation volume ratio is greater than or equal to 1:10, 3) free-islet exposure to pancreatic digestion products is minimized by frequent separation of islets, and 4) collagenase concentration is optimized by titration. Gelation is also minimized by maintaining 5) incubation temperature at 38 +/- 1 degree C and 6) pH in the range 7.7-7.9. Variations in these physical and chemical conditions were analyzed by determining islet yields (stereoscopic microscope counts of serially diluted samples) and by insulin radioimmunoassay of acid alcohol extracts of isolated islets after separation through discontinuous Ficoll gradients. When isolation conditions are optimized as stated, we typically recover 3.3 +/- 1.0 x 10(4) islets/g pancreas, corresponding to greater than 10(6) islets per donor.
Plasma Elastin-derived Peptide Levels in Normal Adults, Children, and Emphysematous Subjects: Physiologic and Computed Tomographic Scan Correlates (1992)

Pulmonary emphysema is likely to be the result of elastic tissue digestion by unrestrained elastase activity in the lung. Elastin breakdown by elastases results in the release of soluble elastin fragments (EDP), which may be measured in plasma by an ELISA. Plasma EDP levels measured using an ELISA were determined in the following groups: disease-free children (n = 24), 0.162 +/- 0.082 ng/ml; disease-free adult nonsmokers (n = 114), 1.74 +/- 0.8 ng/ml; smokers (n = 68), 2.76 +/- 4.59 ng/ml; reformed smokers (n = 43), 1.91 +/- 1.14 ng/ml. Adults with established pulmonary emphysema (n = 50), as defined by bullous formation on the chest radiograph, had levels of 50.83 +/- 24.8 ng/ml, significantly higher than the disease-free groups at p < 0.01. Pulmonary emphysema can be reflected by pulmonary function tests, especially those that measure the pulmonary elastic properties, and by computed tomographic (CT) scan percent emphysema score. We therefore examined the relationship of plasma EDP to these other indicators of pulmonary emphysema in a separate group of 26 subjects using elastic recoil measurements (K), and a further group of 30 subjects with CT scan percent emphysema score. A significant correlation of p < 0.001 was shown for plasma EDP and K and a significant correlation of p < 0.01 was shown for plasma EDP and CT scan percent emphysema score, these correlations suggesting that plasma EDP levels are indicators of the loss of pulmonary distensibility and of mild to moderate pulmonary emphysema. These findings suggest that pulmonary emphysema is characterized by active elastin breakdown.
Cyclic Adenosine 3′,5′-Monophosphate-dependent Protein Kinase of Human Erythrocyte Membranes (1972)

Abstract A cyclic AMP (adenosine 3', 5'-monophosphate)-dependent protein kinase has been found in membranes derived from human erythrocytes which accounts for greater than 70% of the total cyclic AMP-dependent protein kinase activity of these cells. Components of the erythrocyte membranes were phosphorylated by membrane-bound protein kinase, but stimulation of the rate of phosphorylation by cyclic AMP was observed only when histones or protamine served as substrates. The membrane-associated cyclic AMP-dependent protein kinase resembles many soluble protein kinases; it requires 20 mm Mg2+ for maximal activity, has apparent Km values of 26 µg per ml and 8.3 µm for protamine and ATP, respectively, and a Ka for cyclic AMP of 28 nm. Erythrocyte membranes specifically bind cyclic AMP and exhibit a dissociation constant of 3.3 nm for the membrane-cyclic AMP complex. Cyclic AMP-independent protein kinase activity was dissociated from the membrane by treatment with 1 m NH4Cl, but the cyclic AMP-binding activity was retained by the particulate fraction. These findings suggest that the cyclic AMP-binding moiety is firmly integrated into the membrane structure, whereas the catalytic moiety may only be loosely associated with the membrane.
Diagnostic-strategy selection for series systems (1990)

The selection of efficient testing strategies for repairable systems composed of components arranged in series is considered. Two cost models (for perfect and imperfect testing) represent the consequences of possible test realizations. The probability that any particular component is responsible for the failure is derived and used as a basis for the two models. The model for perfect testing is solved exactly. In the optimal perfect-test sequence the components are tested in decreasing order of the ratio of: (probability that the component is responsible for the system failure) to (component test cost). For imperfect testing, possible diagnostic errors are included in a model for which two heuristic solution strategies are provided. The model represents the consequences of both false-positive and false-negative component-test outcomes. The heuristic strategies yield efficient test sequences. Under reasonable assumptions, the second heuristic strategy is guaranteed to locate the optimal test sequence. The model can quantitatively evaluate the benefits of test-accuracy enhancement plans. These models and algorithms provide convenient methods for selecting efficient test-sequences. This is illustrated by representative examples.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Immobilization of Lipase on Single Walled Carbon Nanotubes in Ionic Liquid (2010)

A lipase from Pseudomonas cepacia was immobilized onto single walled carbon nanotubes (SWNTs) in two different ways in each of two solvent systems (buffer and ionic liquid). The most efficient immobilization was achieved in ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, BMIM-<TEX>$BF_4$</TEX>). In this procedure, carbon nanotubes were first functionalized noncovalently with 1-pyrenebutyric acid N-hydroxysuccinimide ester and then subject to the coupling reaction with the lipase in ionic liquid. The resulting immobilized enzyme displayed the highest activity in the transesterification of 1-phenylethyl alcohol in the presence of vinyl acetate in toluene.
Linewidth dependence of electromigration in evaporated Al-0.5%Cu (1980)

The linewidth dependence of electromigration damage has been evaluated for 2.5-cm-long, 1–4-μm-wide, e-gun-evaporated Al-0.5%Cu conductions. It is observed that the ≲1-μm lines are much longer lived than the ≳2-μm lines, reversing the trend at wider widths. These results are rationalized on the basis of the ’’bamboo’’-type grain structure of narrow lines in contrast to the much more heterogeneous structure of the wider meanders.
Role of a copper (I)‐dependent enzyme in the anti‐platelet action of S‐nitrosoglutathione (1996)

1. S-nitrosoglutathione (GSNO) is a potent and selective anti-platelet agent, despite the fact that its spontaneous rate of release of nitric oxide (NO) is very slow. Our aim was to investigate the mechanism of the anti-aggregatory action of GSNO. 2. The biological action of GSNO could be mediated by NO released from S-nitrosocystylglycine, following enzymatic cleavage of GSNO by gamma-glutamyl transpeptidase. The anti-aggregatory potency of GSNO was not, however, altered by treatment of target platelets with the gamma-glutamyl transpeptidase inhibitor acivicin (1 mM). gamma-Glutamyl transpeptidase is not, therefore, involved in mediating the action of GSNO. 3. The rate of breakdown of S-nitrosoalbumin was increased from 0.19 +/- 0.086 nmol min-1 to 1.52 +/- 0.24 nmol min-1 (mean +/- s.e.mean) in the presence of cysteine (P < 0.05, n = 4). Inhibition of platelet aggregation by S-nitrosoalbumin was also significantly increased by cysteine (P < 0.05, n = 4), suggesting that the biological activity of S-nitrosoalbumin is mediated by exchange of NO from the protein carrier to form the unstable compound cysNO. Breakdown of GSNO showed a non-significant acceleration in the presence of cysteine, from 0.56 +/- 0.22 to 1.77 +/- 0.27 nmol min-1 (mean +/- s.e.mean) (P = 0.064, n = 4), and its ability to inhibit platelet aggregation was not enhanced by cysteine. This indicates that the anti-platelet action of GSNO is not dependent upon transnitrosation to form cysNO. 4. Platelets pretreated with the copper (I)-specific chelator bathocuproine disulphonic acid (BCS), then resuspended in BCS-free buffer, showed resistance to the inhibitory effect of GSNO. These findings suggest that BCS impedes the action of GSNO by binding to structures on the platelet, rather than by chelating free copper in solution. 5. Release of NO from GSNO was catalysed enzymatically by ultrasonicated platelet suspensions. This enzyme had an apparent K(m) for GSNO of 12.4 +/- 2.64 microM and a Vmax of 0.21 +/- 0.03 nmol min-1 per 10(8) platelets (mean +/- s.e.mean, n = 5). It was inhibited by BCS, but not by the iron chelator bathophenathroline disulphonic acid, nor by acivicin. 6. We conclude that the stable S-nitrosothiol compound GSNO may exert its anti-platelet action via enzymatic, rather than spontaneous release of NO. This is mediated by a copper-dependent mechanism. The potency and platelet-selectivity of GSNO may result from targeted NO release at the platelet surface.
Improvement of the Trivalent Inactivated Flu Vaccine Using PapMV Nanoparticles (2011)

Commercial seasonal flu vaccines induce production of antibodies directed mostly towards hemaglutinin (HA). Because HA changes rapidly in the circulating virus, the protection remains partial. Several conserved viral proteins, e.g., nucleocapsid (NP) and matrix proteins (M1), are present in the vaccine, but are not immunogenic. To improve the protection provided by these vaccines, we used nanoparticles made of the coat protein of a plant virus (papaya mosaic virus; PapMV) as an adjuvant. Immunization of mice and ferrets with the adjuvanted formulation increased the magnitude and breadth of the humoral response to NP and to highly conserved regions of HA. They also triggered a cellular mediated immune response to NP and M1, and long-lasting protection in animals challenged with a heterosubtypic influenza strain (WSN/33). Thus, seasonal flu vaccine adjuvanted with PapMV nanoparticles can induce universal protection to influenza, which is a major advancement when facing a pandemic.
KMUP‐1 Promotes Osteoblast Differentiation Through cAMP and cGMP Pathways and Signaling of BMP‐2/Smad1/5/8 and Wnt/β‐Catenin (2014)

Phosphodiesterase (PDE) inhibitors have been suggested as a possible candidate for the treatment of osteopenia, including osteoporosis. KMUP-1 is a novel xanthine derivative with inhibitory activities on the PDE 3, 4, and 5 iso-enzymes to suppress the degradation of cAMP and cGMP. This study aimed to investigate the effect of KMUP-1 on osteoblast differentiation and the underlying cellular and molecular mechanisms. Primary osteoblasts and osteoblastic MC3T3-E1 cells were examined. KMUP-1 enhanced alkaline phosphatase (ALP) activity and mineralization compared to untreated controls in primary osteoblasts and MC3T3-E1 cells. KMUP-1 also increased the mRNA expression of the osteoblastic differentiation markers, including collagen type 1a, ALP, osteocalcin, osteoprotegerin, BMP-2, and Runx2, a key transcription regulator for osteoblastic differentiation. The osteogenic effect of KMUP-1 was abolished by BMP signaling inhibitor, noggin. Furthermore, we found that KMUP-1 upregulated Smad1/5/8 phosphorylations with subsequent BRE-Luc activation confirmed by transient transfection assay. In addition, KMUP-1 inactivated glycogen synthase kinase-3β (GSK-3β), with associated nuclear translocation of β-catenin. Co-treatment with H89 and KT5823, cAMP and cGMP pathway inhibitors, respectively, reversed the KMUP-1-induced activations of Smad1/5/8, β-catenin, and Runx2. The findings demonstrate for the first time that KMUP-1 can promote osteoblast maturation and differentiation in vitro via BMP-2/Smad1/5/8 and Wnt/β-catenin pathways. These effects are mediated, in part, by the cAMP and cGMP signaling. Thus, KMUP-1 may be a novel osteoblast activator and a potential new therapy for osteoporosis.
Design of a GPR for deep investigations (2007)

This paper describes the design steps in producing a GPR for deep, mainly geological and environmental investigations. A phase coded spread spectrum pulse is used with high power transmitters to an antenna array. The receiver has a real time data converter with a correlator giving 125dB dynamic range. Initial results are shown.
Assessment of Avenins from Different Oat Varieties Using R5-Based Sandwich ELISA (2017)

Gluten derived from wheat and related triticeae cereals possesses distinct amino acid sequences that provoke the immunopathogenic features of celiac disease (CD) in genetically susceptible individuals. However, the role of oat-derived gluten, or avenins, in CD pathogenesis remains a disputed matter, as evidenced by a lack in harmonized legislation regarding gluten classification in relation to gluten-free labeling. In this study, we have analyzed a panel of pure oat cultivars using a sandwich ELISA based on the R5 monoclonal antibody (mAb), which binds to canonical epitopes occurring within celiagenic peptides present in triticeae-derived gluten but reportedly not present in avenins. We have identified three varieties of oats that reproducibly bind R5 antibodies and levels indicating the presence of gluten at more than the 20 ppm gluten regulatory threshold. Nested assessment using Western blot analysis and alternative gluten detection systems corroborated these results. Collectively, these data suggest that select oat varieties may prove problematic to patients with CD and to food companies and regulatory agencies and will extend our basic understanding of current gluten detection systems.
Lateral <i>pn</i>-junctions as a novel electron detector for microcolumn systems (1999)

A lateral pn-junction detector (l-pn detector) for low energy backscattered electrons has been designed, fabricated, and characterized. General design rules for l-pn detectors have been established. The detector assembly consists of a n-type finger structure diffused in p-type silicon. It was fabricated using standard clean room technologies and tested in a conventional scanning electron microscope under conditions similar to those in electron beam microcolumns. With this setup, images of Au/Si alignment marks were obtained with primary beam currents around 1 nA at 1 keV electron energy. The gain was measured for 1 and 2 keV primary beam energy. Signal to noise ratio and bandwidth were determined from image data. It is demonstrated that l-pn detectors are well suited for imaging in microcolumns.
Molecular cloning and characterization of two rat renal kallikrein genes (1988)

Kallikreins compose a multigene family coding for a subgroup of serine proteases, which are involved in the processing of bioactive peptides. Two rat kallikrein-related genes, RSKG-7 (rat submandibular gland kallikrein gene 7) and RSKG-3, have been cloned and their sequences analyzed. RSKG-7 is approximately 4200 bases in length and consists of five exons and four introns. The 5' end region contains the variant CATAT box and TTTAAA box; the 3' end region contains the polyadenylation signal AATAAA. This gene encodes a putative 28,935-dalton preproenzyme of 261 amino acids (aa). The active enzyme consists of 237 aa and is preceded by a deduced signal peptide of 18 aa and a profragment of 6 aa. RSKG-3 is highly homologous to RSKG-7 in terms of its sequence and structure; it encodes a 28,730-dalton prepropeptide consisting of a signal peptide of 18 aa, a profragment of 6 aa, and an active peptide of 235 aa. Sequence comparisons of RSKG-7, RSKG-3, and other kallikrein-related enzymes reveal the key amino acid residues needed for both serine protease activity (His/Asp/Ser) and kallikrein-like cleavage specificity at basic amino acids. Northern blot analyses using specific oligonucleotide probes demonstrate that, among the 12 tissues studied, RSKG-7 and RSKG-3 are expressed in the rat kidney and submandibular gland. Castration of male rats results in a decrease in submandibular gland RSKG-7 mRNA, which can be restored to the normal level by treatment with thyroxine or testosterone. On the other hand, neither castration nor hormonal manipulation affects RSKG-7 mRNA levels in the kidney.
Cancer/testis antigen expression and autologous humoral immunity to NY-ESO-1 in gastric cancer. (2004)

Gastric cancer has the highest mortality rate and the second-highest morbidity rate of all malignant tumors in China. Since cancer/testis (CT) antigens are expressed in various types of human tumors but generally not in normal tissue except for testis, they are promising antigens for cancer immunotherapy. NY-ESO-1, in particular, is the most immunogenic of the CT antigens. To study the feasibility of developing a CT antigen vaccine for gastric cancer, 101 gastric cancer samples were analyzed for the presence of NY-ESO-1 mRNA and that of 10 other CT antigen genes. Twelve out of 101 samples (11.9%) were found to be NY-ESO-1 mRNA-positive, 11 of them from advanced stage patients. In 7 of the 12 NY-ESO-1 mRNA-positive samples, the NY-ESO-1 protein was also detected by immunohistochemistry. An autologous humoral immune response to NY-ESO-1 was detected in 6 of 12 advanced stage NY-ESO-1 mRNA-positive patients, indicating that NY-ESO-1 is immunogenic in advanced stage gastric cancer. The serum from a patient with an NY-ESO-1 negative but LAGE-1 positive tumor was also found to be NY-ESO-1 antibody positive, possibly due to cross-reactivity between NY-ESO-1 and LAGE-1. All NY-ESO-1 mRNA-positive gastric cancer samples also expressed one to seven additional CT genes, revealing a tendency toward a clustered expression pattern, regardless of disease stage. About 74% of the samples expressed at least one CT antigen, most frequently MAGE-3 (41.6%). NY-ESO-1 and MAGE-3 are thus potential targets for a multivalent CT antigen vaccine.
Self-deoxygenating glassware (2019)

The removal of dissolved oxygen (O2) from solution is a prerequisite for many reactions, frequently requiring specialized equipment/reagents or expertise. Herein, we introduce a range of reusable, shelf-stable enzyme-functionalized glassware, which biocatalytically removes O2 from contained aqueous solutions. The effectiveness of the activated glassware is demonstrated by facilitating several O2-intolerant RAFT polymerizations.
Response to Comment on “Electrokinetic Stacking Injection of Neutral Analytes under Continuous Conductivity Conditions” (2002)

ADVERTISEMENT RETURN TO ISSUEPREVCommentResponse to Comment on "Electrokinetic Stacking Injection of Neutral Analytes under Continuous Conductivity Conditions"Dean S. Burgi and James P. LandersView Author Information Department of Chemistry, University of Virginia, McCormick Road, Charlottesville, Virginia 22901Cite this: Anal. Chem. 2002, 74, 15, 3931Publication Date (Web):July 3, 2002Publication History Published online3 July 2002Published inissue 1 August 2002https://pubs.acs.org/doi/10.1021/ac020355whttps://doi.org/10.1021/ac020355warticle-commentaryACS PublicationsCopyright © 2002 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views313Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (13 KB) Get e-AlertscloseSUBJECTS:Electrical conductivity,Electrokinetics,Electroosmosis,Micelles Get e-Alerts
Dissolution kinetics of calcium carbonate in sea water; III, A new method for the study of carbonate reaction kinetics (1974)

For part 2, see Am. Jour. Sci., Vol. 272, p. 840, 1972; see also this Bibliography, Berner and Morse, 05