gebhart/patrepeval huggingface dataset:
Interactions of Eukaryotic Translation Initiation Factor 3 (eIF3) Subunit NIP1/c with eIF1 and eIF5 Promote Preinitiation Complex Assembly and Regulate Start Codon Selection (2004)

The N-terminal domain (NTD) of NIP1/eIF3c interacts directly with eIF1 and eIF5 and indirectly through eIF5 with the eIF2-GTP-Met-tRNA(i)(Met) ternary complex (TC) to form the multifactor complex (MFC). We investigated the physiological importance of these interactions by mutating 16 segments spanning the NIP1-NTD. Mutations in multiple segments reduced the binding of eIF1 or eIF5 to the NIP1-NTD. Mutating a C-terminal segment of the NIP1-NTD increased utilization of UUG start codons (Sui(-) phenotype) and was lethal in cells expressing eIF5-G31R that is hyperactive in stimulating GTP hydrolysis by the TC at AUG codons. Both effects of this NIP1 mutation were suppressed by eIF1 overexpression, as was the Sui(-) phenotype conferred by eIF5-G31R. Mutations in two N-terminal segments of the NIP1-NTD suppressed the Sui(-) phenotypes produced by the eIF1-D83G and eIF5-G31R mutations. From these and other findings, we propose that the NIP1-NTD coordinates an interaction between eIF1 and eIF5 that inhibits GTP hydrolysis at non-AUG codons. Two NIP1-NTD mutations were found to derepress GCN4 translation in a manner suppressed by overexpressing the TC, indicating that MFC formation stimulates TC recruitment to 40S ribosomes. Thus, the NIP1-NTD is required for efficient assembly of preinitiation complexes and also regulates the selection of AUG start codons in vivo.
Reducible Poly(amido ethylenimine)s Designed for Triggered Intracellular Gene Delivery (2006)

Poly(amido ethylenimine) polymers, a new type of peptidomimetic polymer, containing multiple disulfide bonds (SS-PAEIs) designed to degrade after delivery of plasmid DNA (pDNA) into the cell were synthesized and investigated as new carriers for triggered intracellular gene delivery. More specifically, three SS-PAEIs were synthesized from Michael addition reactions between cystamine bisacrylamide (CBA) and three different ethylene amine monomers, i.e., ethylenediamine (EDA), diethylenetriamine (DETA), or triethylenetetramine (TETA). Complete addition reactions were confirmed by (1)H NMR. The molecular weight, buffer capacity, and relative degree of branching for each SS-PAEI was determined by gel permeation chromatography (GPC), acid-base titration, and liquid chromatography-mass spectroscopy (LC-MS), respectively. Physicochemical characteristics of polymer/pDNA complexes (polyplexes) were analyzed by gel electrophoresis, particle size, and zeta-potential measurements. All three SS-PAEIs effectively complex pDNA to form nanoparticles with diameters less than 200 nm and positive surface charges of approximately 32 mV. The in vitro gene transfer properties of SS-PAEIs were evaluated using mouse embryonic fibroblast cell (NIH3T3), primary bovine aortic endothelial cell (BAEC), and rat aortic smooth muscle cell (A7R5) lines. Interestingly, polyplexes based on all three SS-PAEIs exhibited remarkably high levels of reporter gene expression with nearly 20x higher transfection efficiency than polyethylenimine 25k. The high transfection efficiency was maintained in the presence of 10% serum in the transfection medium. Furthermore, confocal microscopy experiments using labeled pDNA indicated that polyplexes of SS-PAEI displayed greater intracellular distribution of pDNA as compared to PEI, most likely due to environmentally triggered release. Therefore, SS-PAEIs are a new class of transfection agents that facilitate high gene expression while maintaining a low level of toxicity.
RNA Biomarkers: Frontier of Precision Medicine for Cancer (2017)

As an essential part of central dogma, RNA delivers genetic and regulatory information and reflects cellular states. Based on high-throughput sequencing technologies, cumulating data show that various RNA molecules are able to serve as biomarkers for the diagnosis and prognosis of various diseases, for instance, cancer. In particular, detectable in various bio-fluids, such as serum, saliva and urine, extracellular RNAs (exRNAs) are emerging as non-invasive biomarkers for earlier cancer diagnosis, tumor progression monitor, and prediction of therapy response. In this review, we summarize the latest studies on various types of RNA biomarkers, especially extracellular RNAs, in cancer diagnosis and prognosis, and illustrate several well-known RNA biomarkers of clinical utility. In addition, we describe and discuss general procedures and issues in investigating exRNA biomarkers, and perspectives on utility of exRNAs in precision medicine.
Architectures and algorithms for parallel updates of raster scan displays (1982)

P&apos;33615-78-C-1551. The views and conclusions contained in this document are those of the authors and should not bc interpreted as representing the official policies, either expressed or implied, of the The frame buffer memory organization is the key to achieving high display performance. Traditional frame buffer designs use the scan-line organization which allows several pixels along the length of a scan-line to be updated together. This thesis advocates the symmetric square organization which allows the access of square regions of the display. This organization is based on the belief that the regions of the display which are commonly accessed simultaneously are no more likely to be tall and thin than to be short and wide. The square memory organization is studied for representative display applications and is shown to be indeed better than the scan-line organization. Based on the algorithms for these applications, a display design is presented. This design uses one custom designed LSI chip, 64 copies of which are required to implement the frame buffer memory system. TABI,Eo[: CONTENTS
Thin Layer Chromatography in Phytochemistry (2008)

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first sourc
The influence of recycled expanded polystyrene (EPS) on concrete properties: Influence on flexural strength, water absorption and shrinkage (2013)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Jamaleddin Elsalah, Yosra Al-Sahli, Ahmed Akish, Omar Saad, Abdurrahman Hakemi; The influence of recycled expanded polystyrene (EPS) on concrete properties: Influence on flexural strength, water absorption and shrinkage. AIP Conference Proceedings 16 December 2013; 1569 (1): 181–185. https://doi.org/10.1063/1.4849254 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Improved <sup>18</sup>F Labeling of Peptides with a Fluoride-Aluminum-Chelate Complex (2010)

We reported previously the feasibility to radiolabel peptides with fluorine-18 ((18)F) using a rapid one-pot method that first mixes (18)F(-) with Al(3+) and then binds the (Al(18)F)(2+) complex to a NOTA ligand on the peptide. In this report, we examined several new NOTA ligands and determined how temperature, reaction time, and reagent concentration affected the radiolabeling yield. Four structural variations of the NOTA ligand had isolated radiolabeling yields ranging from 5.8% to 87% under similar reaction conditions. All of the Al(18)F NOTA complexes were stable in vitro in human serum, and those that were tested in vivo also were stable. The radiolabeling reactions were performed at 100 degrees C, and the peptides could be labeled in as little as 5 min. The IMP467 peptide could be labeled up to 115 GBq/micromol (3100 Ci/mmol), with a total reaction and purification time of 30 min without chromatographic purification.
Low cost solder flip chip (2002)

Since the advent of flip chip packaging technology in the solid logic technology (SLT) of IBM in the early 1960s, a great deal of thought and energy has been invested toward making flip chip cost competitive with wirebonding. While this goal has been occasionally met in the intervening years, the relentless progress of wirebond technology has repeatedly regained the advantage.
Polypeptide signalling for plant defence genes. (1994)

The synthesis of proteinase inhibitor proteins in response to wounding is a defensive response against pest and pathogen attacks. Wounding of leaves results in the release of a mobile wound signal which induces proteinase inhibitor synthesis throughout the plant. The signal transduction pathway regulating this response is not fully understood, but several compounds have been identified which are capable of inducing proteinase inhibitor synthesis in tomato and potato leaves. These compounds include cell wall fragments of both plant and pathogen origin [1-3], abscisic acid [4], jasmonic acid [5] and, most recently, an 18-amino-acid polypeptide, called systemin, isolated from tomato leaves [6]. In this chapter, we describe the properties of systemin and its precursor prosystemin, and we summarize the evidence supporting a role for systemin as an initial signal that regulates proteinase inhibitor synthesis in response to wounding.
Ghrelin: Structure and Function (2005)

Small synthetic molecules called growth hormone secretagogues (GHSs) stimulate the release of growth hormone (GH) from the pituitary. They act through the GHS-R, a G protein-coupled receptor whose ligand has only been discovered recently. Using a reverse pharmacology paradigm with a stable cell line expressing GHS-R, we purified an endogenous ligand for GHS-R from rat stomach and named it "ghrelin," after a word root ("ghre") in Proto-Indo-European languages meaning "grow." Ghrelin is a peptide hormone in which the third amino acid, usually a serine but in some species a threonine, is modified by a fatty acid; this modification is essential for ghrelin's activity. The discovery of ghrelin indicates that the release of GH from the pituitary might be regulated not only by hypothalamic GH-releasing hormone, but also by ghrelin derived from the stomach. In addition, ghrelin stimulates appetite by acting on the hypothalamic arcuate nucleus, a region known to control food intake. Ghrelin is orexigenic; it is secreted from the stomach and circulates in the bloodstream under fasting conditions, indicating that it transmits a hunger signal from the periphery to the central nervous system. Taking into account all these activities, ghrelin plays important roles for maintaining GH release and energy homeostasis in vertebrates.
Identification of Rapid Turnover Transcripts Overexpressed in Thyroid Tumors and Thyroid Cancer Cell Lines: Use of a Targeted Differential RNA Display Method to Select for mRNA Subsets (1997)

The mRNAs of transiently expressed proteins such as cytokines and proto-oncogenes are commonly subject to rapid transcriptional activation and degradation. Transcript turnover is determined in part by association of certain proteins with consensus AU-rich motifs (AUUUA) in the 3'-untranslated region of the transcripts. Here we report a modification of differential RNA display (DRD) to detect differentially expressed rapid turnover mRNAs containing AU-rich motifs from thyroid cancer tissues and cell lines. RNA of normal and thyroid cancer tissues was differentially displayed using a 3'anchor primer to the poly(A) tail and an arbitrary 5'primer incorporating an AUUUA sequence. The appropriateness of the strategy was established by its ability to display known early response genes, such as c- fos, using partially degenerate primers. To test whether the novel cDNAs isolated coded for transcripts subject to rapid turnover, they were used as probes for Northern blots of RNA from clonal human thyroid carcinoma cell lines treated for varying periods with either cycloheximide or actinomycin D. A number of novel differentially expressed cDNA fragments were isolated from human papillary thyroid carcinoma tissues, among them a cDNA with zinc finger motifs and homology to other zinc finger proteins. Using this fragment to probe a cDNA library, a full-length cDNA (ZnF20) was isolated that was 4333 bp in length and contained an open reading frame of 1029 amino acids. The ZnF20 cDNA hybridized to multiple transcripts in a thyroid cancer cell line (8.0, 4.5 and 2 kb) that increased after cycloheximide treatment and decayed <2 h after addition of actinomycin D. The ZnF20 mRNA was overexpressed in three of six thyroid papillary carcinomas as compared with paired normal thyroid tissue controls. The data presented here support the use of a targeted DRD approach for the isolation of rapid turnover mRNAs, many of which may be interesting candidate oncogenes.
UWB radar sensor to monitor heart physiology (2011)

Feasibility for noncontact monitoring of major cardiovascular physiology using ultra-wideband radar sensors is studied in computer simulations. A full-wave electromagnetic model of a human chest with a heart of variable blood filling volume is developed. The model is then simulated for several discrete volumes of heart blood filling. Computed subsequent radar returns are modulated by heart motions at different cardiac phases but heavily masked by background scattering. The recorded radar returns are processed using the developed algorithm to extract physiological information that can be linked to ventricle heart blood volume and heart electrical activity associated with ECG.
An innate-like Vδ1 <sup>+</sup> γδ T cell compartment in the human breast is associated with remission in triple-negative breast cancer (2019)

Innate-like tissue-resident γδ T cell compartments capable of protecting against carcinogenesis are well established in mice. Conversely, the degree to which they exist in humans, their potential properties, and their contributions to host benefit are mostly unresolved. Here, we demonstrate that healthy human breast harbors a distinct γδ T cell compartment, primarily expressing T cell receptor (TCR) Vδ1 chains, by comparison to Vδ2 chains that predominate in peripheral blood. Breast-resident Vδ1<sup>+</sup> cells were functionally skewed toward cytolysis and IFN-γ production, but not IL-17, which has been linked with inflammatory pathologies. Breast-resident Vδ1<sup>+</sup> cells could be activated innately via the NKG2D receptor, whereas neighboring CD8<sup>+</sup> αβ T cells required TCR signaling. A comparable population of Vδ1<sup>+</sup> cells was found in human breast tumors, and when paired tumor and nonmalignant samples from 11 patients with triple-negative breast cancer were analyzed, progression-free and overall survival correlated with Vδ1<sup>+</sup> cell representation, but not with either total γδ T cells or Vδ2<sup>+</sup> T cells. As expected, progression-free survival also correlated with αβ TCRs. However, whereas in most cases TCRαβ repertoires focused, typical of antigen-specific responses, this was not observed for Vδ1<sup>+</sup> cells, consistent with their innate-like responsiveness. Thus, maximal patient benefit may accrue from the collaboration of innate-like responses mounted by tissue-resident Vδ1<sup>+</sup> compartments and adaptive responses mounted by αβ T cells.
Newer Synthetic Rubbers Derived from Olefins and Diolefins (1961)

Abstract Synthetic rubbers are of significant interest to the petroleum industry, since nearly all of the raw materials required in their production are petrochemical in nature. Many of these petrochemicals are produced in large quantities for other chemical end uses as well. Among the most important of these large-tonnage intermediates are butadiene, styrene, acrylonitrile, chloroprene and isobutylene. The newer developments in synthetic rubber, to which this paper will largely be devoted, have focused greater attention on other monomers, such as isoprene, ethylene and propylene. The following figures will give some idea of the present and future consumption of these raw materials, largely based on petroleum, for chemical manufacture:
Essential components of growth and development. (1995)

The Joint Commission on Accreditation of Healthcare Organizations' standards require that documentation of age-appropriate care be done when a child or adolescent is treated, with particular attention given to growth and development, socialization, and other issues. The principles of growth and development from infancy to adolescence are discussed, including physical, psychological, and psychosocial needs. Nursing implications are presented to improve understanding of growth and developmental stages as well as provision of nursing care.
Brachytherapy in the treatment of solitary colorectal metastases to the liver (1990)

It is well-established that 30-40% of patients with solitary liver metastases from primary colorectal tumors can be cured by resection. Conventional radiation therapy has had only a palliative role in treating liver metastases because the dose that the liver will tolerate is far below a tumoricidal dose. In contrast, brachytherapy allows one to deliver a tumoricidal dose to the tumor while limiting the dose to surrounding normal tissue to the tolerance dose. As a pilot study, 125I seeds were implanted into unresectable hepatic metastases, or positive margins of resection, at the time of surgery. This report concerns six patients whose liver lesions were the only known site of disease and in whom precipitous drops in carcinembryonic antigen (CEA) levels followed the implants. Recurrence was observed in only one of the 11 implanted site, with a median follow-up of 12 months.
The intelligent container — What can MEMS do for logistics of food? (2015)

One third of the food produced on earth is never eaten, but lost. A considerable loss of food during transport could be prevented, if modern sensor technology would be used to manage the supply chain. The Intelligent Container project aims at reducing losses of food in transport by using sensor and communication technology and to improve supply chain management. The environment of the fruit during transport is observed concerning temperature, humidity and acceleration. The remaining shelf life time is continuously estimated. The status of ripening and decay of fruit is monitored looking at ethylene - a gaseous ripening indicator of many fruit. New micro gas chromatography systems are developed for the ethylene detection. For autonomous decisions, shelf life estimators and other decision support tools are embedded in the sensor network.
Identification of tumor associated immune responses against brachyury, a transcription factor and driver of EMT, in chordoma patients receiving a yeast-brachyury vaccine (gi-6301) (2014)

Meeting abstracts Brachyury is a tumor-associated antigen and transcription factor that drives the epithelial-to-mesenchymal transition (EMT) in human carcinomas. The aim of this study was to assess whether patients with chordoma, a rare tumor of the notochord that over-expresses brachyury, can
Vaccines Against Drug Abuse (2011)

The currently available medications for the treatment of drug abuse have had only limited success. Anti-addiction vaccines, aimed at eliciting antibodies that block the pharmacological effects of drugs, have great potential for treating drug abuse. We review the status of two vaccines that are undergoing clinical trials (for cocaine and nicotine addiction) and two that are still in preclinical development (for methamphetamine and heroin addiction). We also outline the challenges and ethical concerns associated with the development of anti-addiction vaccines and their use as future therapeutics.
A multi channel high accuracy real time DAQ system for fast neutron spectrometer based on GEM-TPC (2012)

To sample the 720 channels signals with 100ns rising edge by over 60dB dynamic range, a 12bits 50Ms/s real time DAQ system has been designed for the Fast Neutron Spectrometer Based on GEM-TPC. This DAQ system is consisted of eight 64 channels FADC cards sampling the signals from the GEM-TPC and communicating with the computer. Caused by the randomness of the events and the large variation range of the data volume (nearly 60dB) from the GEM-TPC, a bit intelligent functions need to be designed to meet the requirement of the real time data acquisition with nearly lkls event rate (each event will generate 1~120 signals with 0.25~3.5us pulse width). The 'complete digital design method' and the 'concatenate encoding method' are also applied in this DAQ system to keep the stability and the high accuracy for the signals processing and communicating. With the design mentioned above, this DAQ system could adapt over 2k/s event rate and over 60dB dynamic range.
Physical limitations on antennas of arbitrary shape (2007)

In this paper, physical limitations on bandwidth, realized gain, Q -factor and directivity are derived for antennas of arbitrary shape. The product of bandwidth and realizable gain is shown to be bounded from above by the eigenvalues of the long-wavelength, high-contrast polarizability dyadics. These dyadics are proportional to the antenna volume and are easily determined for an arbitrary geometry. Ellipsoidal antenna volumes are analysed in detail, and numerical results for some generic geometries are presented. The theory is verified against the classical Chu limitations for spherical geometries and shown to yield sharper bounds for the ratio of the directivity and the Q -factor for non-spherical geometries.
Robust GNSS Spoof Detection using Direction of Arrival: Methods and Practice (2018)

GNSS spoofing is a growing concern due to the increasing use of GNSS in safety and economically important applications. The foremost task to manage the GNSS spoofing threat is detection. Indeed, many potential means and measurements have been proposed for spoof detection. However, there exist no panaceas. Rather, we should rely on many different detection means to provide robust detection. One measurement useful for detection is direction of arrival (DOA), which we can get from many sources such as array and dual polarization antenna (DPA). Even with accurate DOA measurements, good monitor tests are needed to make an accurate decision or determination of spoofing. This paper develops and examines monitor tests suitable for DOA based spoof detection. Developed tests aim to have provable and conservative performance levels making them suitable for safety of life applications. Several tests are developed including signal pair comparisons based on statistical hypothesis tests, Bayesian estimates as well as comprehensive all satellites in view comparisons. On-air spoofing results from a DPA are used to validate performance. The developed techniques must detect simple cases such as when all examined signals are from a single spoofer (“all spoof” case) and challenging cases where there is a mix of genuine signals and spoofed signals (“mixed spoof” case). Another consideration is that measurements will typically be relative DOA as platform attitude may or may not be known. Solving the relative DOA problem also captures the simpler case where we have some knowledge of platform attitude (i.e. absolute DOA).