gebhart/patrepeval huggingface dataset:
In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery (2004)

Targeted gene transfection remains a crucial issue to permit the real development of genetic therapy. As such, in vivo targeted transfection of specific subsets of hematopoietic stem cells might help to sustain hematopoietic recovery from bone marrow aplasia by providing local production of growth factors. Balb/C mice were injected intravenously, with an anti-mouse c-kit (CD117) monoclonal antibody chemically coupled to a human IL-3 gene-containing plasmid DNA. Mice were sacrificed for tissue analyses at various days after injection of the conjugates. By ELISA, the production of human IL-3 was evidenced in the sera of animals 5 days after treatment. Cytofluorometric analysis after in vivo transfection of a reporter gene eGFP demonstrated transfection of CD117+/Sca1+ hematopoietic immature cells. By PCR analysis of genomic DNA and RNA using primer specific pIL3 sequences, presence and expression of the human IL-3-transgene were detected in the bone marrow up to 10 days in transfected mice but not in control animals. These data clearly indicate that antibody-mediated endocytosis gene transfer allows the expression of the IL-3 transgene into hematopoietic immature cells, in vivo. While availability of marketed recombinant growth factors is restricted, this targeting strategy should permit delivery of therapeutic genes to tissues of interest through systemic delivery. In particular, the ability to specifically target growth factor expression into repopulating hematopoietic stem cells may create new opportunities for the treatment of primary or radiation-induced marrow failures.
Lymphocyte, monocyte, and natural killer cell reference ranges in postpartal women (1997)

Normative values for immune-cell subsets in postpartal women, who are recovering from the relative immunosuppression of pregnancy, have not been established. Considerable differences in normative values for subsets of immune cells have been demonstrated based on sociodemographic factors, such as age and race. In order to make accurate clinical decisions about postpartal women, comparisons with normal reference ranges are necessary. Therefore, flow cytometric data for 51 healthy women at 4 months postpartum are presented and changes over the first 4 postpartal months are documented. The levels of some lymphocyte cell subsets, such as CD4+/CD45RA+ and Ia on lymphocytes, remained stable over time. The levels of other lymphocyte cell subsets, such as CD4+/CD29+, increased over the first 4 postpartal months, while those of other cell subsets, such as CD8 and CD11b, increased between delivery and 2 months postpartum and then dropped again by the fourth postpartal month. The levels of two natural killer cell subsets (CD3-/CD16+ and CD3-/CD57+) rose from delivery until 1 month postpartum and then plateaued. Comparisons were made with reference ranges of nonpostpartal groups provided in the literature and in a study of healthy women being conducted in the same laboratory, and postpartal women were found to have lower values of CD8, CD3-/CD16+, CD4+/CD45RA+, CD20, and CD11b than those reported in the literature.
Selective Separation of Actinide(III) by a rPr-BTP/nitrobezene Extraction System (2008)

A selective separation of Actirlide(III) by a nPr-BTP/nitrobezene extraction system was studied. The nPr-BTP (2.6-Bis-(5.6-n-propyl-1.2.4-triazin-3-yl)-pyridine) of a environmentally -friendly CHN type was self-synthesized and its compatability with diluent and stability with nitric acid were investigated. At the 0.1M nPr-BTP/nitrobenzene-1M and O/A=2, extraction yields of Am used as a representative of Actinide(III) and Eu were about 85% and 8%, respectively, and the other RE elements such as Nd, Ce and Y were extracted less than 3% (separation factor of Am and Eu was about 60). Thus, there was no problems in the selective extraction of Actinide(III) from RE. The stripping yield of Am with 0.05M at O/A= 1, however, was about 43% and the maximum stripping yield was 65% at O/A=0.3. It is necessary to develop the stripping system including the stripping agent instead of nitric acid solution.
Ring opening polymerization ofD,L-lactide in the presence of zinc metal and zinc lactate (1998)

The polymerization of D,L-lactide in the presence of zinc metal was investigated for low values of the monomer/catalyst ratio in order to allow end-group characterization of the growing chains by high resolution 360MHz 1H NMR. The results showed that the polymerization was moisture sensitive and that only a fraction of zinc was active. Small quantities of a side-product were detected and identified as zinc lactate. This compound appeared to be an efficient initiator of the ring-opening polymerization in the bulk. Zinc lactate is thus proposed as the actual initiator of the polymerization of 1,4-dioxane-2,5-diones in the presence of zinc metal. A polymerization mechanism based on a cationic process co-catalysed by lactic acid is also proposed, which accounted very satisfactorily for experimental data. Initiation by zinc lactate yielded high molecular weight polymers with a high degree of conversion and rather high polymerization rates. Therefore, zinc lactate appears to be an attractive alternative to the initiations by zinc metal and stannous octoate, the latter being suspected of some toxicity. © 1998 SCI.
Treatment Benefit in the Enhanced External Counterpulsation Consortium (2000)

The present study utilized a cohort of 2,289 consecutive patients enrolled in the Enhanced External Counterpulsation (EECP) Consortium to evaluate whether results of university studies showing EECP safety and effectiveness in treating angina can be generalized. EECP was found to be safe and well tolerated with a 4.0% rate of adverse experiences. Angina class improved in 74% of patients with limiting angina (Canadian Cardiovascular Society, CCS, functional class II-IV), with patients most impaired at baseline demonstrating the greatest improvement (39.5% of patients in CCS III and IV improved 2 or more classes). Efficacy was independent of provider setting or experience, women responded as well as men, and although younger patients demonstrated a greater likelihood of improvement, EECP was effective in patients ranging from 19 to 97 years. Extending the benefit of EECP treatment to a wider range of patients may be indicated based on these findings.
Part-Based Visual Tracking with Online Latent Structural Learning (2013)

Despite many advances made in the area, deformable targets and partial occlusions continue to represent key problems in visual tracking. Structured learning has shown good results when applied to tracking whole targets, but applying this approach to a part-based target model is complicated by the need to model the relationships between parts, and to avoid lengthy initialisation processes. We thus propose a method which models the unknown parts using latent variables. In doing so we extend the online algorithm pegasos to the structured prediction case (i.e., predicting the location of the bounding boxes) with latent part variables. To better estimate the parts, and to avoid over-fitting caused by the extra model complexity/capacity introduced by the parts, we propose a two-stage training process, based on the primal rather than the dual form. We then show that the method outperforms the state-of-the-art (linear and non-linear kernel) trackers.
Image-Analyzing Microscopes (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImage-Analyzing MicroscopesPhilip G. SteinCite this: Anal. Chem. 1970, 42, 13, 103A–107APublication Date (Print):November 1, 1970Publication History Published online23 May 2012Published inissue 1 November 1970https://pubs.acs.org/doi/10.1021/ac60295a810https://doi.org/10.1021/ac60295a810research-articleACS PublicationsRequest reuse permissionsArticle Views11Altmetric-Citations3LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Coccidioidomycosis in the Dog (1980)

A 10-month-old male Scotch Terrier was admitted to Stange Memorial Clinic on Nov. 12, 1952. The history given revealed that in Arizona, about five weeks previously, the dog's appetite had failed and a cough was evidenced. The patient was taken to an Arizona veterinary hospital for examination and was retained there for one week. A diagnosis of "Desert Fever" with a swelling of a lymph node near the heart was made. Medication in Arizona consisted of aureomycin, terramycin and chloromycetin in unknown dosages. The dog was brought back to Iowa three weeks prior to admittance to Stange Memorial Clinic, and upon arrival here, the general condition was noted as poor. A temperature of 103.4°F., listlessness, poor appetite, weakness, constipation and a serous discharge from the eyes were recorded.
On the Autoxidation of Bitter-Tasting Iso-α-acids in Beer (2010)

The iso-alpha-acids, the major contributor to bitter beer taste, is well-known to strongly degrade during beer aging. The storage of beer in brown glass bottles revealed a strong depletion of the trans-configured isomers in a highly specific manner, whereas the corresponding cis-iso-alpha-acids seemed to be hardly affected. In comparison, storage of beer in polyethylene terephthalate bottles, which are known to be permeable to oxygen, induced a drastic degradation of both isomers independent of their cis/trans configuration. To investigate the chemical transformation of iso-alpha-acids under oxidative storage conditions, suitable model experiments were performed, and the reaction products that formed were identified as previously not reported hydroperoxy- and hydroxyl-allo-iso-alpha-acids by means of one-/two-dimensional NMR and liquid chromatography-mass spectrometry experiments; for example, cis- and trans-configured hydroperoxy-alloisohumulone as well as the corresponding hydroxy-alloisohumulones were generated upon oxidation of cis- and trans-isohumulone independent of their cis/trans configuration. In addition, the oxidation products formed from the various iso-alpha-acid congeners were quantitatively determined in a series of beer samples stored under defined conditions. For the first time, these data help to understand the molecular mechanism involved in the autoxidative degradation of iso-alpha-acids in beer.
The bactericidal/permeability-increasing protein (BPI), a potent element in host-defense Against gram-negative bacteria and lipopolysaccharide (1993)

The bactericidal/permeability-increasing protein (BPI), is a ca. 55 kDa cytotoxic cationic protein of polymorphonuclear leukocytes (PMN) that is present principally in the azurophilic granules. BPI is toxic only toward Gram-negative bacteria. This target specificity is attributable to the strong attraction of BPI for the lipopolysaccharides (LPS) in the bacterial envelope. BPI also binds with high affinity (apparent Kd 2-5 nM) to a broad range of LPS species and potently inhibits the biologic activities of LPS in vitro. A proteolytically prepared or recombinant ca 25 kDa N-terminal fragment of BPI carries all the antibacterial activities of holo-BPI and is more potent than the holo-protein against more resistant bacteria with S-form LPS in their envelope. The fragment is as active as holo-BPI as an LPS-neutralizing agent in vitro and more potently inhibits cytokine induction by S-form Escherichia coli in whole blood ex vivo. Recombinant forms of both proteins protect animals against the lethal effects of administered LPS.
Reduction of the naturally occurring motor neuron loss by enlargement of the periphery (1976)

Motor hyperplasia following the enlargement of the periphery by implantation of a supernumerary leg is not due to "remote control" of proliferation, as shown by motor neuron counts in 6-day chick embryos. We have tested the alternative hypothesis that we are dealing with reduction of the naturally occurring cell death. In normal development, the lumbar lateral motor column (l.m.c.) undergoes motor neuron degeneration resulting in a cell loss of at least 40%, which occurs between six and one-half and nine and one-half days. Following transplantation of supernumerary legs, cases selected for vigorous motility showed a numerical difference between experimental and contralateral (control) sides amounting to +11.0% to +27.5%. The transplants were innervated by varying combinations of thoracic and rostral lumbar nerves. We interpret our data in terms of survival of motor neurons which normally would have failed in a competition at the periphery but which were sustained by the enlarged peripheral fields. Our data do not permit a decision between the two alternatives: competition for synaptic sites or for a trophic agent. The surviving motor neurons are not limited to the rostral segments of the motor column but in most instances distributed along its entire rostro-caudal extent, implying a redistribution of all l.m.c. axons. The term "hyperplasia" is no longer appropriate for the phenomenon under consideration and should be replaced by the term "hypothanasia.""
Borehole images for assessing present day stresses (2013)

Abstract Borehole images, which are now commonly acquired in the oil industry highlight very precisely damage that affects the borehole wall. Two damage types exist. The first corresponds to drilling operations: corkscrew effect, keyseat, cave etc. The second is related to present day stresses: induced fractures, breakouts or shear on preexisting planes. In addition to the real borehole damage, the acquisition and the processing of the image data may bring confusion in the identification of what is due to stresses. A new processing and filtering method is proposed for identifying the stress induced features and measuring precisely their geometry. Borehole images of two wells drilled in a shaly-sand reservoir particularly tight of the Timimoun basin (Algeria) clearly show that the main parameter that controls the damage geometry is the lithology. The stiffer and tighter is the rock the wider and deeper are the breakouts. To explain this strange behavior it is proposed that long term plasticity redistributes the horizontal tectonic stresses between the layers as a function of their shaliness. Another borehole image from Hassi Messaoud reservoir (Algeria) exhibits strong and progressive variation of the stress state where the well crosses a fault. By analogy to outcrops this perturbation is interpreted as due to a local deviation of the fault plane geometry and not to the average fault orientation itself. Such perturbations are unpredictable apart from images acquired while drilling. Once stress induced features are clearly identified we examine how they can be used for assessing the present day stresses. In a vertical well it is well known that breakouts or induced fractures just provide the azimuth of the principal horizontal stresses as the vertical stress, parallel to the well surface, has no effect on the damage location. In a deviated well the three principal stresses influence breakouts and induced fracture location. Mastin [1988] has clearly demonstrated where breakouts will appear in deviated wells. This location depends on the three following parameters: the horizontal stress azimuth, which stress is vertical and R the shape of the stress tensor. These parameters are exactly the ones that also control the orientation of the shear on a preexisting plane. As a consequence, the observation of a breakout or an induced fracture in a single deviated well does not bring conclusive information on the stress state. But the combination of observations in multiple deviation situations may lead to a clear stress state definition. This is similar to the methods promoted by Jacques Angelier in which slickenside inversion is used for determining the stress tensor. The breakout inversion method applied to 7 wells of a north Ural field drilled with very different azimuth and deviation leads to clear definition of the local stress state. Once the stress tensor parameters are clearly identified, it becomes possible to predict borehole or perforation stability for any well deviations.
Fungi occurring on water hyacinth [Eichhornia crassipes (Mart.) Solms] in Kerala. (2004)

Water hyacinth is one of the most invasive aquatic weeds of Kerala. A survey was conducted in the waterways of four southern districts of Kerala to document the fungal pathogens of water hyacinth. Out of the 21 fungi recorded, 17 were pathogenic. Among these, Myrothecium advena Sacc. is a new report on water hyacinth. Furthermore, M. advena and Fusarium pallidoroseum (Cooke) Sacc. caused more than 50% infection of the weed and hold promise as biocontrol agents of water hyacinth.
A new approach for sequencing human IRS1 phosphotyrosine‐containing peptides using CapLC‐Q‐TOF<sup>micro</sup> (2005)

Reversible phosphorylation of proteins functions as a biological switching network for activation and inhibition of downstream biological processes. Since phosphorylations of these sites are often transient processes, and hence sub-stoichiometric, systematic characterization of phosphorylation sites is a formidable challenge. In this work, a new approach was developed to pinpoint phosphotyrosine sites on tyrosine-containing peptides. This required (1) the development of a new and highly sensitive nano-electrospray assembly and (2) validation of the concept that the specificity and detection limit for trace levels of phosphotyrosine immonium ion in peptide mixtures from protein digests can be increased by varying the collision energy. With our method, an automatic tandem mass spectrometric analysis of peptides eluted from a C(18) capillary liquid chromatographic column is triggered by a positive confirmation of phosphotyrosine immonium ion in a time-of-flight mass spectrometric survey. The approach was tested by analyzing the phosphorylation of human IRS-1 peptides that interact with the Src-homology 2 domain and mixtures of these peptides with tryptic digests of bovine serum albumin and horse heart myoglobin.
Chemical determination of the m1 Moloney sarcoma virus pP60gag gene order: evidence for unique peptides in the carboxy terminus of the polyprotein. (1978)

The gene order of the ml Moloney sarcoma virus (mlMSV) specific pP60gag (P60) was determined by direct chemical analysis of the polyprotein. P60 was cleaved with cyanogen bromide (CNBr) into eight partial and complete fragments ranging in mass from 10,000 daltons to 58,000 daltons. Peptide maps of these fragments were compared to maps of p15, p12, and three CNBr fragments of p30. The polarity of p15 and p12 in a CNBr fragment of P60 was determined by carboxypeptidase A digestion; likewise the CNBr fragments of p30 were ordered by aminopeptidase digestion. The linear arrangement of P60 CNBr fragments gave the gene order of NH2-p15-p12-p30-COOH. The m3 isolate of MSV expresses a P70 gag polyprotein. Peptide maps of 48,000-dalton CNBr fragments of m3 P70 and ml P60 were similar and suggested that both polyproteins were similar through the NH2-terminal two-thirds of p30. However, the presence of peptides unique to the 10,500-dalton COOH-terminal fragment of m1MSV p30 and not present in the p30 of either m3MSV or Moloney leukemia virus suggested that the gag gene deletion in the m1 isolate begins in the p30 reading frame.
Co-trafficking of HFE, a Nonclassical Major Histocompatibility Complex Class I Protein, with the Transferrin Receptor Implies a Role in Intracellular Iron Regulation (1998)

The mechanism by which a novel major histocompatibility complex class I protein, HFE, regulates iron uptake into the body is not known. HFE is the product of the gene that is mutated in >80% of hereditary hemochromatosis patients. It was recently found to coprecipitate with the transferrin receptor (Feder, J. N., Penny, D. M., Irrinki, A., Lee, V. K., Lebron, J. A., Watson, N., Tsuchihashi, Z., Sigal, E., Bjorkman, P. J., and Schatzman, R. C. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 1472-1477; Parkkila, S., Waheed, A., Britton, R. S., Bacon, B. R., Zhou, X. Y., Tomatsu, S., Fleming, R.E. , and Sly, W. S. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 13198-13202) and to decrease the affinity of transferrin for the transferrin receptor (Feder et al.). In this study, HeLa cells were transfected with HFE under the control of the tetracycline-repressible promoter. We demonstrate that HFE and the transferrin receptor are capable of associating with each other within 30 min of their synthesis with pulse-chase experiments. HFE and the transferrin receptor co-immunoprecipitate throughout the biosynthetic pathway. Excess HFE is rapidly degraded, whereas the HFE-transferrin receptor complex is stable. Immunofluorescence experiments indicate that they also endocytose into transferrin-positive compartments. Combined, these results suggest a role for the transferrin receptor in HFE trafficking. Cells expressing HFE have modestly increased levels of transferrin receptor and drastically reduced levels of ferritin. These results implicate HFE further in the modulation of iron levels in the cell.
A Fully Implicit, Three-Dimensional, Two-Phase, Control-Volume Finite Element Model for the Simulation of Naturally Fractured Reservoirs (1996)

Abstract This paper describes the formulation and application of a fully implicit, three-dimensional, two-phase (water and oil) mathematical model for the simulation of naturally fractured reservoirs. The model equations are discretized using the control-volume finite element (CVFE) scheme where pressure terms are treated in a finite element manner while mobility terms are upstream-weighted as in the usual finite difference (FD) method. The form of the discretized equations is similar to the usual FD method, thus standard techniques are used to solve the Jacobian. The equations are solved fully implicit for pressure, saturation, and wellbore variables using the iterative Newton-Raphson method of solution. Two field scale examples, that were first presented by Kazemi et al., were used to test the model. These include a quadrant of a five-spot pattern, and a five-well fractured reservoir having dip and natural water influx.
Actin Cytoskeletal Disruption following Cryopreservation Alters the Biodistribution of Human Mesenchymal Stromal Cells In Vivo (2014)

Mesenchymal stromal cells have shown clinical promise; however, variations in treatment responses are an ongoing concern. We previously demonstrated that MSCs are functionally stunned after thawing. Here, we investigated whether this cryopreservation/thawing defect also impacts the postinfusion biodistribution properties of MSCs. Under both static and physiologic flow, compared with live MSCs in active culture, MSCs thawed from cryopreservation bound poorly to fibronectin (40% reduction) and human endothelial cells (80% reduction), respectively. This reduction correlated with a reduced cytoskeletal F-actin content in post-thaw MSCs (60% reduction). In vivo, live human MSCs could be detected in murine lung tissues for up to 24 hr, whereas thawed MSCs were undetectable. Similarly, live MSCs whose actin cytoskeleton was chemically disrupted were undetectable at 24 hr postinfusion. Our data suggest that post-thaw cryopreserved MSCs are distinct from live MSCs. This distinction could significantly affect the utility of MSCs as a cellular therapeutic.