gebhart/patrepeval huggingface dataset:
Purification and properties of<i>Thermus filiformis</i>DNA polymerase expressed in<i>Escherichia coli</i> (1999)

The gene encoding Thermus filiformis (Tfi) DNA polymerase was expressed under the control of the tac promoter on a high-copy plasmid, pJR, in Escherichia coli. The Tfi DNA polymerase was purified by using heat treatment and DEAE-Sephacel column chromatography. The purified enzyme had a molecular mass of 92 kDa, as estimated by SDS/PAGE. The optimum pH and temperature of the enzyme were 8.4-9.0 and 70-72.5 degrees C respectively. The half-life of the enzyme at 94 degrees C was approx. 40 min. The enzyme was activated by the bivalent cations, Mg(2+) and Mn(2+), and was inhibited by EDTA. The optimal Mg(2+) concentration of the enzyme was 4 mM. The optimal conditions for the PCR reaction were slightly different from those for the enzyme activity except for the optimal Mg(2+) concentration. Low concentrations of KCl had no effect on either the enzymic activity or the PCR amplification. The result of the PCR experiment with the enzyme indicates that Tfi DNA polymerase might be useful in DNA amplification.
Modulation of Ia and leukocyte common antigen expression in rat glomeruli during the course of glomerulonephritis and aminonucleoside nephrosis. (1984)

We have investigated changes in the glomerular expression of two antigens, Ia and leukocyte common (LC) antigens, during the course of two models of glomerular injury in the rat. Acute glomerulonephritis induced by nephrotoxic, antiglomerular basement membrane antiserum is accompanied by a 3-fold increase in the number of glomerular cells expressing Ia and LC antigens. Acquisition of the Ia+ phenotype appears to occur in LC+ monocytes that have assumed subendothelial and intramesangial positions. Enhanced Ia expression is seen over a wide range of proteinuria. Decomplementation prior to nephrotoxic, antiglomerular basement membrane anti-serum administration abrogates both the proteinuria and the increased Ia expression; an increase in cells expressing LC antigen still occurs. The chronic phase of nephrotoxic, antiglomerular basement membrane antiserum-induced glomerulonephritis is associated with a persistent increase in glomerular Ia+ cells residing primarily in the mesangium. In the glomerulopathy induced by the aminonucleoside of puromycin, there are two phases of glomerular Ia modulation. In the first week, there is a decrease in Ia expression by LC+ cells. The second week is marked by infiltration by monocytes and an increase in Ia expression by both resident and infiltrating cells. Modulation of the Ia phenotype on resident and infiltrating marrow-derived cells carries implications with respect to the immunologic pathogenesis of both models of glomerular injury.
Crystal Structure of an ATPase-active Form of Rad51 Homolog from Methanococcus voltae (2004)

Homologous gene recombination is crucial for the repair of DNA. A superfamily of recombinases facilitate a central strand exchange reaction in the repair process. This reaction is initiated by coating single-stranded DNA (ssDNA) with recombinases in the presence of ATP and Mg(2+) co-factors to form helical nucleoprotein filaments with elevated ATPase and strand invasion activities. At the amino acid sequence level, archaeal RadA and Rad51 and eukaryal Rad51 and meiosis-specific DMC1 form a closely related group of recombinases distinct from bacterial RecA. Unlike the extensively studied Escherichia coli RecA (EcRecA), increasing evidences on yeast and human recombinases imply that their optimal activities are dependent on the presence of a monovalent cation, particularly potassium. Here we present the finding that archaeal RadA from Methanococcus voltae (MvRadA) is a stringent potassium-dependent ATPase, and the crystal structure of this protein in complex with the non-hydrolyzable ATP analog adenosine 5'-(beta,gamma-iminotriphosphate), Mg(2+), and K(+) at 2.4 A resolution. Potassium triggered an in situ conformational change in the ssDNA-binding L2 region concerted with incorporation of two potassium ions at the ATPase site in the RadA crystals preformed in K(+)-free medium. Both potassium ions were observed in contact with the gamma-phosphate of the ATP analog, implying a direct role by the monovalent cations in stimulating the ATPase activity. Cross-talk between the ATPase site and the ssDNA-binding L2 region visualized in the MvRadA structure provides an explanation to the co-factor-induced allosteric effect on RecA-like recombinases.
Evidence that a 16-kilodalton integral membrane protein antigen from Schistosoma japonicum adult worms is a type A2 phospholipase (1991)

Type A2 phospholipase (PLA2) activity has been observed in integral membrane protein extracts of Schistosoma japonicum. Antiserum raised against bee venom PLA2 recognized a single 16-kDa band in the parasite extracts; it also localized to antigen in the gut lining of fixed adult schistosomes as shown by immunofluorescence techniques. Evidence was obtained that the molecule was expressed at low levels in comparison with other integral membrane proteins and was weakly immunogenic in rabbits. Two oligonucleotide probes were constructed on the basis of highly conserved regions between the nucleotide sequences of rat, bovine, rattlesnake, and bee venom PLA2; these probes were used to isolate S. japonicum genomic DNA phage clones. A 1.8-kb FnuD2 fragment was shown by Southern blot analysis to strongly hybridize with the 5' 32P-labeled PLA2 oligonucleotides in both S. japonicum genomic DNA and DNA from one of the phage clones. The nucleotide and predicted amino acid sequences of this fragment revealed homology with the C terminus of PLA2s from different species.
Validity of the Lie Detector (1988)

The profession of polygraphy uses physiological measures to improve the detection of deception. The science most relevant for assessing the utility of these measures is that of psychophysiology. This article examines the validity of polygraphy from the perspective of the science of psychophysiology, which employs physiological measures to study and differentiate psychological processes. The focus is on the version practiced currently by most members of the American Polygraph Association, the “control question technique” (CQT). A brief consideration of some critical terms is followed by a description of CQT polygraphy, and then by a review of the literature. We conclude that as a scientific tool, CQT polygraphy is of questionable validity, although it is probably a better-than-chance detector of guilt.
Generation of a three-dimensional collagen scaffold-based model of the human endometrium (2020)

The endometrium is the secretory lining of the uterus that undergoes dynamic changes throughout the menstrual cycle in preparation for implantation and a pregnancy. Recently, endometrial organoids (EO) were established to study the glandular epithelium. We have built upon this advance and developed a multi-cellular model containing both endometrial stromal and epithelial cells. We use porous collagen scaffolds produced with controlled lyophilization to direct cellular organization, integrating organoids with primary isolates of stromal cells. The internal pore structure of the scaffold was optimized for stromal cell culture in a systematic study, finding an optimal average pore size of 101 µm. EO seeded organize to form a luminal-like epithelial layer, on the surface of the scaffold. The cells polarize with their apical surface carrying microvilli and cilia that face the pore cavities and their basal surface attaching to the scaffold with the formation of extracellular matrix proteins. Both cell types are hormone responsive on the scaffold, with hormone stimulation resulting in epithelial differentiation and stromal decidualization.
An Unusual Isotope Effect on Substrate Inhibition in the Oxidation of Arachidonic Acid by Lipoxygenase (2003)

Soybean lipoxygenase catalyzes the oxidation of arachidonic acid to 15S-HPETE. The reaction displays strong substrate inhibition with unlabeled substrate but no discernible substrate inhibition with arachidonic acid labeled with deuterium at C13, the site of hydrogen/deuterium atom abstraction. The unusual behavior is due primarily to a large kinetic isotope effect on Km,O2 as a result of the strong selection against deuterium in the abstraction step.
Magnetic Microactuation Of Torsional Polysilicon Structures (2005)

A microactuator technology utilizing magnetic thin films and polysilicon flexures is applied to torsional micro structures. These structures are constructed in a batch-fabrication process that combines electroplating with conventional IC-lithography, materials, and equipment. A microactuated mirror made from a 430 x 130 x l5 /spl mu/m/sup 3/ nickel-iron plate attached to a pair of 400 x 2.2 x 2.2 /spl mu/m/sup 3/ polysilicon torsional beams has been rotated more than 90/spl deg/ out of the plane of the wafer and actuated with a torque greater than 3.0 nN-m. The torsional flexure structure constrains motion to rotation about a single axis which can be an advantage for a number of microphotonic applications (e.g., beam chopping, scanning, and steering).
Studies of Synthetic Polymers by Nonradiative Energy Transfer (1988)

Nonradiative energy transfer between fluorescent labels attached to polymers has been used to characterize polymer miscibility, the interpenetration of chain molecules in solution, micelle formation in graft copolymers, the unfolding of collapsed chain molecules in polymer melts, and the transfer of energy absorbed by a large number of donor labels to a small number of acceptors by an "antenna effect." The change in the emission spectrum after ionomer solutions with different fluorescent counterions were mixed provided rate constants for counterion interchange. The fluorescence behavior of dispersions of donor-labeled polymers stabilized by a graft copolymer with acceptor fluorophores in the solution phase led to inferences about the morphology of the dispersed particles.
Technique to generate equal amplitude, higher-orderopticalpulses in rational harmonically modelocked fibre ring lasers (2001)

A novel technique of pulse amplitude equalisation in higher-order optical pulses in a rational harmonically modelocked fibre ring laser is proposed and demonstrated. The combination of intra-cavity optical filtering, and even-order modulation sideband generation using nonlinear characteristics of a Mach-Zehnder intensity modulator can effectively eliminate pulse amplitude instability at higher-order rational harmonic modelocking. The generated optical pulse trains exhibit equal amplitudes with a high degree of spectral purity in terms of a large suppression of supermode noise, low amplitude noise, phase noise, and timing jitter.
Quinine is bactericidal. (2009)

The concomitant antimalarial and antibacterial action of Quinine dihydrochloride may be beneficial in developing countries adding to cost effectiveness of treatment provided to patients belonging to low socioeconomic group.
ORGANIC GASTRIC SYPHILIS (1930)

W1'ith Special l'late
Effect of oxygen on magnetic properties of Nd-Fe-B magnets (1988)

Oxygen plays a very important role in the magnetic properties of R-Fe-B-type alloys. As the oxygen content increases in Nd-Fe-B and Nd-Dy-Fe-Al-B alloys, the alloys gradually lose their magnetic and thermomagnetic properties: the Hci decreases and both the temperature coefficient of Hci and the irreversible temperature loss increase. The degradation of thermomagnetic properties may be accounted for by depletion of the Nd-rich phase in the grain boundaries through Nd oxidation. High coercivity magnets, relatively stable with respect to temperature, can be produced by minimizing the oxygen content in R-Fe-B alloys.
A first book of quantum field theory (2000)

PRELIMINARIES Why Quantum Field Theory Creation and Annihilation Operators Special Relativity Space and Time in Relativistic Quantum Theory Natural Units CLASSICAL FIELD THEORY A quick review of particle mechanics Euler-Lagrange Equations in Field Theory Hamiltonian Formalism Noether's Theorem QUANTIZATION OF SCALAR FIELDS Equation of Motion The Field and its Canonical Quantization Fourier Decomposition of the Field Ground State of the Hamiltonian and Normal Ordering Fock Space Complex Scalar Field Propagator QUANTIZATION OF DIRAC FIELDS Dirac Hamiltonian Dirac Equation Plane Wave Solutions of Dirac Equation Projection Operators Lagrangian for a Dirac Field FourIer Decomposition of the Field Propagator THE S-MATRIX EXPANSION Examples of Interactions Evolution Operator S-Matrix Wick's Theorem FROM WICK EXPANSION TO FEYNMAN DIAGRAMS Yukawa Interaction: Decay of a Scalar Normalized States Sample Calculation of a Matrix Element Another Example: Fermion Scattering Feynman Amplitude Feynman Rules Virtual Particles Amplitudes which are not S-Matrix Elements CROSS SECTIONS AND DECAY RATES Decay Rate Example of Decay Rate Calculation Scattering Cross Section Generalities of 2-to-2 Scattering Inelastic Scattering with 4-Fermion Interaction Mandelstam Variables QUANTUM ELECTRODYNAMICS Local Gauge Invariance Interaction Hamiltonian Lower Order Processes Electron-Electron Scattering Electron-Positron Scattering e-e+ (R)m-m+ Consequence of Gauge Invariance Compton Scattering Scattering by an External Field Bremmsstrahlung P, T, C, AND THEIR COMBINATIONS Motivations from Classical Physics Parity Charge Conjugation Time Reversal CP CPT ELECTROMAGNETIC FORM FACTORS General Electromagnetic Vertex Physical Interpretation of Form Factors Anomalous Magnetic Moment of the electron Charge Form Factor Electron-Proton Scattering RENORMALIZATION Degree of Divergence of a Diagram Specific Examples in QED Outline of the Program Ward-Takahashi Identity General Forms for Divergent Amplitudes Regularization of Self-Energy Diagrams Counterterms Full Lagrangian Observable Effects of Renormalization SYMMETRIES AND SYMMETRY BREAKING Classification of Symmetries Groups and Symmetries Approximate Symmetries Spontaneous Breaking of Symmetries Goldstone's Theorem Higgs Mechanism YANG-MILLS THEORY OF NON-ABELIAN GAUGE FIELDS Gauge Fields of Non-Abelian Symmetry Pure Gauge Lagrangian Interactions of Non-Abelian Gauge Fields Equations of Motion and Conserved Currents Quantization of Non-Abelian Gauge Fields Quantum Chromodynamics STANDARD ELECTROWEAK THEORY Gauge Group Spontaneous Symmetry Breaking Fermions in the Theory Gauge Boson Decay Scattering Processes Propagator for Unstable Particles Global Symmetries of the Model APPENDIX: Useful Formulas INDEX
Activation of β-catenin Signaling Pathways by Classical G-Protein-Coupled Receptors: Mechanisms and Consequences in Cycling and Non-cycling Cells (2006)

Wnt signaling pathways are some of the most intensely studies in all of biology. Recently, a number of classical heterotrimeric G protein coupled receptors (GPCRs) have been shown to activate the canonical Wnt pathway, culminating in the stabilization of beta-catenin and induction of transcription of genes regulated by the Tcf/Lef family of transactivators. However, mechanisms by which these GPCRs accomplish this differ in key ways, and in some circumstances, the phenotypes produced are novel. Herein, we will examine mechanisms by which classical GPCRs interact with the canonical Wnt pathway, culminating in its activation, and describe the consequences of this activation, focusing on the heart. In the heart, the contractile cells, or cardiomyocytes, are terminally differentiated and virtually exclusively grow by increasing cell size (hypertrophy) rather than cell number, and we will describe how GPCR-mediated activation of the canonical pathway can drive this process.
Modern wax technology. (1949)

ADVERTISEMENT RETURN TO ISSUEArticleNEXTModern wax technology.Hubert W. Jenkins and Harold B. Fried Cite this: J. Chem. Educ. 1949, 26, 4, 182Publication Date (Print):April 1, 1949Publication History Received3 August 2009Published online1 April 1949Published inissue 1 April 1949https://doi.org/10.1021/ed026p182RIGHTS & PERMISSIONSArticle Views179Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (4 MB) Get e-Alerts Get e-Alerts
Common Technical Specification of G-PON system among major worldwide access carriers (2006)

This paper will show how many of the major worldwide access carriers cooperated to minimize the cost of G-PON implementation by clarifying their requirements at near/medium term and identifying commonalities among these requirements (e.g. optical transmission, split ratio, optical budget, line rate, GTC, QoS, Service mapping) thanks to a G-PON CTS document that has been finally approved in February 2006.
Automatic Control in Anesthesia (1985)

This report is divided into two parts. First, we developed two new servo control systems by modifying an existing one. The original system was designed to control inspired halothane concentration using mean arterial pressure; the two new systems were designed to control inspired halothane concentration using end-tidal concentration or to control mean arterial pressure using the automated infusion of nitroprusside. Second, we compared the performance of experienced physician and nurse anesthetists (nine, six, and six experiments, respectively). The experiments incorporated a standardized testing sequence of two changes in desired blood pressure (set point) and two pharmacologically induced disturbances in blood pressure (perturbations). The scoring was designed to examine how fast blood pressure changed (90% response time), how far past the set point it went (overshoot), how long it took to eliminate most of the fluctuations in blood pressure (settling time), and the degree of fluctuation of blood pressure after settling (stability). Given three systems to be tested, there were (3 X 14) 42 possible mean scores for the machine and 42 for the anesthetists. The machine scored better than the anesthetists in 38 out of 42 of the mean scores; the differences were statistically significant in 19 out of 42 scores. The wide scatter in performances of the anesthetists prevented the achievement of significance in nine cases with large differences between means. Thus when the scores from the three systems were combined to achieve a larger n value, the machine outperformed the anesthetist in 12 out of 14 scores.(ABSTRACT TRUNCATED AT 250 WORDS)
Site-Selective Doping of Compound Semiconductors by Ion Implantation of Radioactive Nuclei (1980)

Sn impurity atoms have been selectively inserted on the two different substitutional lattice sites in the III-V semiconductors GaP, GaAs, GaSb, InP, InAs, and InSb. Radioactive $^{119}\mathrm{In}^{+}$ and $^{119}\mathrm{Sb}^{+}$ ions which decay to the M\"ossbauer state of $^{119}\mathrm{Sn}$ have been implanted. From the isomer shifts determined in M\"ossbauer-emission experiments it is concluded that the implanted In and Sb ions selectively populate III and V sites, respectively.
Further Comments on the Characteristics of the Hedeman H-1, H-2, and H-3 Codes (1983)

This letter clarifies some of the properties of the Hedeman codes and examines their application to band-limited channels. Attention is drawn to the equivalence of H-2 and H-3 to simple linear modulation schemes. All three codes are dc free by virtue of a charge constraint which additionally ensures a minimum distance two (considered as rate one-half codes). The asymptotic error performance at high signal-to-noise ratio can thus be similar to NRZ or biphase signaling, although the Viterbi algorithm is required to achieve this performance in the case of H-1 code. Like Miller code, both H-1 and H-2 will pass through unequalized low-pass channels with bandwidths little greater than required for NRZ signaling.
Asynchronous secure computation (1993)

We initiate a study of security in asynchronous networks.
A New Stelè from Athens (1902)

Apparently there had been sculptured on the missing half of the marble stelè lately acquired by the British Museum from Athens a seated figure with hand upraised (Pl. 1.). There is a trace of the raised arm and also of a footstool. The subject had therefore been one of those scenes of parting or meeting so common on Athenian stelae. But the young man leaning on his staff is not of the usual Athenian type. In several respects he resembles a youthful Heracles on a relief from Mt. Ithome now in Athens, which figure it has been the custom to regard as Polycleitan (Fig. 1). So far as the pose of the head and the Diadumenos-like modelling of the body are concerned that opinion may be right. Only we must remember that the somewhat formal modelling of the thorax both in the Ithome relief and the new stelè is not unfrequent in Greek art, at least from the time of Lysippos onwards. A familiar instance is the Hermes on the sculptured drum of a column from Ephesus in the British Museum. It is a modification of the type of Polycleitos and may have set in much earlier than Lysippos. It may even have extended to Athens.
An experimental 180 Mb/sec PRML channel for magnetic recording (1991)

An experimental 180 Mbit/s partial-response maximum-likelihood (PRML) channel was developed in order to investigate various technical issues arising in very high speed magnetic disk recording systems. The channel was implemented with discrete analog and logic components. A thin-film 10-turn write head and an experimental high-resolution magnetoresistive read head were used with a high coercivity, thin-film disk. Linear densities of 4000 fc/mm were achieved at 45 m/s and 100 nm flying height. The authors describe the experimental channel in detail and also indicate some of the new experimental results obtained.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Neutrophil maturation rate determines the effects of dipeptidyl peptidase 1 inhibition on neutrophil serine protease activity (2016)

Following inhibition of DPP1 in the rat, the NSP activity was determined by the amount of DPP1 inhibition and the turnover of neutrophils and is thus supportive of the role of neutrophil maturation in the activation of NSPs. Clinical trials to monitor the effect of a DPP1 inhibitor on NSPs should take into account the delay in maximal response on the one hand as well as the potential delay in a return to baseline NSP levels following cessation of treatment.