gebhart/patrepeval huggingface dataset:
Expression of two different endogenous galactoside-binding lectins sharing sequence homology. (1988)

The endogenous beta-D-galactoside-binding lectins of UV-2237-IP3 fibrosarcoma cells consist of two polypeptides with molecular weights of 14,500 (L-14.5) and 34,000 (L-34). Recently we constructed complementary DNA (cDNA) libraries from these cells in the expression vector lambda gt11 and isolated and characterized cDNA clones encoding the two lectin species (A. Raz et al., Exp. Cell Res., 173: 109, 1987). We report here on the similarity in structure and gene number in genomic DNA of the fibrosarcoma cells and syngeneic normal mouse lung DNA. The expression of mRNAs hybridizing to these cDNAs was evaluated in three pairs of normal versus their transformed cell variants. Messenger RNA corresponding to the Mr 34,000 lectin was present more abundantly in the transformed cells than in their normal counterparts, while no difference was detected between the cell pairs with respect to mRNA levels corresponding to the Mr 14,500 lectin. We have established the nucleotide sequences of the two cDNA clones. The deduced amino acid sequence of L-14.5 lectin cDNA clone (L-3) is significantly homologous to the sequence of six other L-14 vertebrate galactoside binding lectins. The sequence of the Mr 34,000 lectin cDNA clone (M5) revealed that it shares a stretch of 39 amino acids with all of the L-14 galactoside binding lectins, irrespective of origin of the species, suggesting both that they are all derived from a common ancestral gene, and that this domain is necessary for similar sugar binding properties.
Efficient expression of a transgene in platelets using simian immunodeficiency virus‐based vector harboring glycoprotein Ibα promoter: <i>in vivo</i> model for platelet‐targeting gene therapy (2006)

Platelets release several mediators that modify vascular integrity and hemostasis. In the present study, we developed a technique for efficient transgene expression in platelets in vivo and examined whether this targeted-gene-product delivery system using a platelet release reaction could be exploited for clinical applications. Analysis of luciferase reporter gene constructs driven by platelet-specific promoters (the GPIIb, GPIbalpha, and GPVI) revealed that the GPIbalpha promoter was the most potent in the megakaryoblastic cell line UT-7/TPO and human CD34+-derived megakaryocytes. Transduction of UT-7/TPO; CD34+-derived megakaryocytes; and c-Kit+, ScaI+, and Lineage- (KSL) murine hematopoietic stem cells with a simian immunodeficiency virus (SIV)-based lentiviral vector carrying eGFP resulted in efficient, dose-dependent expression of eGFP, and the GPIbalpha promoter seemed to bestow megakaryocytic-specific expression. Transplantation of KSL cells transduced with SIV vector containing eGFP into mice showed that there was preferable expression of eGFP in platelets driven by the GPIbalpha promoter [7-11% for the cytomeglovirus (CMV) promoter, 16-27% for the GPIbalpha promoter]. Furthermore, transplantation of ex vivo-transduced KSL cells by SIV vector carrying human factorVIII (hFVIII) driven by the GPIbalpha promoter induced the production of detectable transcripts of the hFVIII gene and the hFVIII antigen in bone marrow and spleen for at least 90 days and partially corrected the hemophilia A phenotype. Platelet-targeting gene therapy using SIV vectors appears to be promising for gene therapy approaches toward not only inherited platelet diseases but also other hemorrhagic disorders such as hemophilia A.
Stabilization of Montmorillonite Clay in Porous Media by Polyacrylamides (1996)

Abstract The critical salinity concentration (CSC) was used to compare the ability of nonionic polyacrylamides (PAM) and cationic polyacrylamides (CPAM) to stabilize montmoriilonite clay dispersed in sandpacks. The method consists of injecting brine at decreasing salinity levels until clay release is detected by a continuous increase in pressure drop. As expected, because of the neutralization of negative clay surface charges by adsorbed macromolecules, CPAM have a higher stabilizing power than PAM, lowering the CSC of NaCl from 27 500 to only 500 ppm, and the CSC of KCI from 5000 to 1000 ppm. However, because of their ionicity and very high adsorption level, CPAM strongly reduce sandpack permeability. Likewise, a low-molecular-weight PAM may be preferred because of its good stabilizing power associated with a minimal loss of core permeability.
Orbit allocation of domestic communications satellites (1969)

An efficient method of packing domestic communications satellites is described. In this method, inclined elliptical synchronous orbits are used instead of inclined circular synchronous orbits. The total number of communications satellites is, for example, 8.4 times larger than that for the equatorial system only.
On Stability of Large-Scale Systems under Structural Perturbations (1973)

A large-scale system isconsidered asasystem constituted ofsubsystems whichmay beconnected ordisconnected fromeach otherduring operation. A newconcept ofconnective stability isintro- ducedbywhich alarge-scale system isregarded asstable ifitremains stable (in thesense ofLyapunov) understructural perturbations produced bytheon-off participation ofthesubsystems. Algebraic conditions are developed thatguarantee exponential connective stability oflarge-scale systems whichmaybecomposed oflinear andnonlinear time-varying subsystems coupled bylinear ornonlinear connections.
Evaluation of In‐Season Nitrogen Management Strategies for Corn Production (2008)

Nitrogen is an important and costly input for corn ( Zea mays L.) production. With rising prices and environmental concerns, producers are looking for ways to better manage N fertilization. A project designed to evaluate in‐season N management strategies for adjusting N rate was conducted in 30 Iowa cornfields from 2004 to 2006. Nitrogen rates applied preplant or early sidedress (PRE‐N) and corn plant sensing with a chlorophyll meter (CM) to detect N stress was used to determine as‐needed in‐season post sensing nitrogen application (POST‐N). Field‐length strips were replicated three times with six N treatments: zero‐N control, 67 kg N ha −1 (reduced PRE‐N rate), 67+ (reduced PRE‐N rate plus POST‐N), 134 kg N ha −1 (agronomic PRE‐N rate), 134+ (agronomic PRE‐N rate plus POST‐N), and 268 kg N ha −1 (PRE‐N non‐N limiting reference rate). Nitrogen deficiency stress sensing was partially successful, with 70 to 80% correct N deficiency detection. Mean yield increased 0.5 Mg ha −1 across all sites with the POST‐N applied to the 67 kg N ha −1 PRE‐N rate. The 67+ POST‐N strategy had a similar total N applied as the agronomic 134 kg N ha −1 PRE‐N rate, but the yield was 0.5 Mg ha −1 lower. This indicates yield potential loss and poor recovery from the POST‐N applications. Economic return comparisons indicated that the agronomic 134 PRE‐N rate and 134+ POST‐N in‐season strategy were similar and had higher return than the 67+ POST‐N strategy. The agronomic PRE‐N rate, with confirmation of N stress and determination of additional N need through plant sensing, was a more cost effective in‐season strategy than starting with the reduced PRE‐N rate.
The Last RNA-Binding Repeat of the <i>Escherichia coli</i> Ribosomal Protein S1 Is Specifically Involved in Autogenous Control (2000)

The ssyF29 mutation, originally selected as an extragenic suppressor of a protein export defect, has been mapped within the rpsA gene encoding ribosomal protein S1. Here, we examine the nature of this mutation and its effect on translation. Sequencing of the rpsA gene from the ssyF mutant has revealed that, due to an IS10R insertion, its product lacks the last 92 residues of the wild-type S1 protein corresponding to one of the four homologous repeats of the RNA-binding domain. To investigate how this truncation affects translation, we have created two series of Escherichia coli strains (rpsA(+) and ssyF) bearing various translation initiation regions (TIRs) fused to the chromosomal lacZ gene. Using a beta-galactosidase assay, we show that none of these TIRs differ in activity between ssyF and rpsA(+) cells, except for the rpsA TIR: the latter is stimulated threefold in ssyF cells, provided it retains at least ca. 90 nucleotides upstream of the start codon. Similarly, the activity of this TIR can be severely repressed in trans by excess S1, again provided it retains the same minimal upstream sequence. Thus, the ssyF stimulation requires the presence of the rpsA translational autogenous operator. As an interpretation, we propose that the ssyF mutation relieves the residual repression caused by normal supply of S1 (i.e., that it impairs autogenous control). Thus, the C-terminal repeat of the S1 RNA-binding domain appears to be required for autoregulation, but not for overall mRNA recognition.
Hyaluronan release from Streptococcus pyogenes: export by an ABC transporter (2004)

Capsular hyaluronan of Streptococcus pyogenes is synthesized at the protoplast membrane. It is widely assumed that hyaluronan is exported by the synthase itself and that no additional protein is required for transfer through plasma membranes. However, we produced an insertional mutation that reduced the mucoid phenotype, hyaluronan production, and capsule formation. Nucleotide sequence analysis of the insertion site identified a gene coding for a protein with an ATP-binding cassette (ABC) that belonged to an ABC transporter system and was located next to the hyaluronan synthesis genes. The mucoid phenotype was reconstituted by complementation with DNA encoding the ABC transporter system. These results indicated that an ABC transporter was required for efficient capsule production.
RATE OF AND RISK FACTORS FOR ACUTE INPATIENT MORTALITY AFTER ORTHOPAEDIC SURGERY (2002)

Death is rare after orthopaedic operations. In the United States, the rate of acute mortality after inpatient orthopaedic surgical procedures is approximately 1% for all patients, 3.1% for patients with a hip fracture, and 0.5% for patients without a hip fracture. These data will aid orthopaedic surgeons in predicting operative mortality for their patients.
Personalized Recommender Systems Integrating Social Tags and Item Taxonomy (2009)

The social tags in web 2.0 are becoming another important information source to profile users' interests and preferences to make personalized recommendations. To solve the problem of low information sharing caused by the free-style vocabulary of tags and the long tails of the distribution of tags and items, this paper proposes an approach to integrate the social tags given by users and the item taxonomy with standard vocabulary and hierarchical structure provided by experts to make personalized recommendations. The experimental results show that the proposed approach can effectively improve the information sharing and recommendation accuracy.
Exceptional Thermal Stability in a Supramolecular Organic Framework: Porosity and Gas Storage (2010)

Reaction of β-amino-β-(pyrid-4-yl)acrylonitrile with the aromatic dicarboxaldehydes 9,10-bis(4-formylphenyl)anthracene and terephthalaldehyde affords the dihydropyridyl products 9,10-bis(4-((3,5-dicyano-2,6-dipyridyl)dihydropyridyl)phenyl)anthracene (L(1)) and 1,4-bis(4-(3,5-dicyano-2,6-dipyridyl)dihydropyridyl)benzene (L(2)), respectively. In the solid state [L(1)]·2.5DMF·3MeOH (SOF-1) crystallizes in the monoclinic space group P2(1)/c and forms a 3D stable supramolecular organic framework via strong N-H···N(py) hydrogen bonds and π-π interactions. The material incorporates pyridyl-decorated channels and shows permanent porosity in the solid state. The pore volumes of the desolvated framework SOF-1a calculated from the N(2) isotherm at 125 K and the CO(2) isotherm at 195 K are 0.227 and 0.244 cm(3) g(-1), respectively. The N(2) absorption capacity of SOF-1a at 77 K is very low, with an uptake of 0.63 mmol g(-1) at 1 bar, although saturation N(2) adsorption at 125 K is 6.55 mmol g(-1) (or 143 cm(3) g(-1)). At ambient temperature, SOF-1a shows significant CO(2) adsorption with approximately 3 mol of CO(2) absorbed per mole of host at 16 bar and 298 K, corresponding to 69 cm(3) g(-1) at STP. SOF-1a also adsorbs significant amounts of C(2)H(2), with an uptake of 124 cm(3) (STP) g(-1) (5.52 mmol g(-1)) at 1 bar at 195 K. Methane uptake at 195 K and 1 bar is 69 cm(3) (STP) g(-1). Overall, gas adsorption measurements on desolvated framework SOF-1a reveal not only high capacity uptakes for C(2)H(2) and CO(2), compared to other crystalline molecular organic solids, but also an adsorption selectivity in the order C(2)H(2) > CO(2) > CH(4) > N(2). Overall, C(2)H(2)(270 K)/CH(4)(273 K) selectivity is 33.7 based on Henry's Law constant, while the C(2)H(2)(270 K)/CO(2)(273 K) ratio of uptake at 1 bar is 2.05. The less bulky analogue L(2) crystallizes in the triclinic space group P1 as two different solvates [L(2)]·2DMF·5C(6)H(6) (S2A) and [L(2)]·2DMF·4MeOH (S2B) as pale yellow tablets and blocks, respectively. Each L(2) molecule in S2A participates in two N-H···O hydrogen bonds between dihydropyridyl rings and solvent DMF molecules. Packing of these layers generates 1D nanochannels along the crystallographic a and b axes which host DMF and benzene molecules. In S2B, each L(2) ligand participates in hydrogen bonding via an N-H···O interaction between the N-H of the dihydropyridyl ring and the O of a MeOH and also via an N···H-O interaction between the N center of a pyridine ring and the H-O of a second MeOH molecule. The presence of the L(2)-HOMe hydrogen bonds prevents ligand-ligand hydrogen bonding. As a result, S2B crystallizes as one-dimensional chains rather than as an extended 3D network. Thermal removal of solvents from S2A results in conversion to denser phase S2C which shows no effective permanent porosity.
Localization of a Normal Prostatic Secretory Product Using the Monoclonal Antibody KR-P8 (1985)

We previously reported that the monoclonal antibody KR-P8 detects a prostate organ-specific antigen that is distinct from other markers such as prostatic acid phosphatase and prostate specific antigen. In this report we demonstrate that the antigen recognized by KR-P8 is among the secretory products of both normal and malignant prostatic epithelium. Immunoperoxidase staining patterns showed that the antigen was concentrated on the luminal surfaces of the glandular epithelial cells of prostate, and also that the antigen was localized within secretory vacuoles of cells of the prostate tumor line PC3. In addition, using an immunoblotting assay the KR-P8 binding antigen was detected in the growth media of PC3 cells and also found to be present in human urine and seminal plasma. The data suggest that the antigen recognized by KR-P8 may be a useful marker for studying the secretory processes of the prostate gland.
Near-infrared surface plasmon polariton dispersion control with hyperbolic metamaterials (2013)

We demonstrate experimentally signatures and dispersion control of surface plasmon polaritons from 1 to 1.8 µm using periodic multilayer metallo-dielectric hyperbolic metamaterials. The fabricated structures are comprised of smooth films with very low metal filling factor. The measured dispersion properties of these hyperbolic metamaterials agree well with calculations using transfer matrix, finite-difference time-domain, and effective medium approximation methods despite using only 2.5 periods. The enhancement factor in the local photonic density of states from the studied samples in the near-infrared wavelength region is determined to be 2.5-3.5. Development of this type of metamaterial is relevant to sub-wavelength imaging, spontaneous emission and thermophotovoltaic applications.
Physical Chemical Studies of Soluble Antigen—Antibody Complexes. I. The Valence of Precipitating Rabbit Antibody<sup>1</sup> (1952)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhysical Chemical Studies of Soluble Antigen—Antibody Complexes. I. The Valence of Precipitating Rabbit Antibody1S. J. Singer and Dan H. CampbellCite this: J. Am. Chem. Soc. 1952, 74, 7, 1794–1802Publication Date (Print):April 1, 1952Publication History Published online1 May 2002Published inissue 1 April 1952https://pubs.acs.org/doi/10.1021/ja01127a052https://doi.org/10.1021/ja01127a052research-articleACS PublicationsRequest reuse permissionsArticle Views78Altmetric-Citations51LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Suspended minerogenic particle distributions in high‐energy coastal environments: Optical implications (2004)

This paper examines suspended minerogenic particle distributions in the near‐coastal ocean, Oceanside, California. The environment is dominated by resuspension of particles from a well‐sorted sandy sediment. We obtain information on the suspended mineral matter from the relocatable numerical sedimentation model, TRANS98. Inputs for the model include bottom particle size distribution, wind speed, wave period and height, and near‐bottom current speed. Model output is a vertical profile of mineral particle concentrations and size distributions at selected depths. The mineral particle size distribution allows the calculation of the total scattering coefficient of suspended minerogenic matter. The particle size distribution for the bed at Oceanside is approximately Gaussian lognormal in character. The predicted size distribution near bottom is a skewed Gaussian lognormal; then the distribution shifts toward the increasing importance of smaller size classes as we approach the water surface. Deeper stations maintain a lognormal character up into the surface layers while the shallower stations switch to a particle size distribution of the smallest size class being the modal class in the surface layers. Hyperbolic size distributions have been proposed for the Oceanside site from optical observations. These distributions would have a slope of 2.1 for coastal water which would decrease to as low as 1.3 for shallow nearshore stations and near the bottom. None of the proposed hyperbolic slopes provides an adequate approximation of the particle distributions predicted by TRANS98. The larger particle size classes are significantly overestimated by hyperbolic models, which causes the total scattering coefficient of the minerogenic matter to be overestimated. These results indicate that hyperbolic slope models cannot be used to retrieve particle size distributions by inversions involving the total scattering coefficient nor are they accurate estimators of the minerogenic scattering coefficient.
Performance Optimization of Parallel Programs - Tracing, Zooming, Understanding - (1995)

Performance optimization most often is based on the detailed knowledge of program behavior. One option to get this information is tracing. The visualization environment PARvis developed at KFA translates a given trace file generated on CRAY T3D into a variety of graphical views, e.g., state diagrams, activity charts, timeline displays, and statistics. PARvis supports an animation mode that can help to locate performance bottlenecks, and it provides flex- ible filter operations to reduce the amount of information displayed. Moreover, it has a powerful zooming feature that allows to identify problems at any level of detail.
The Importance of Accurate Blood Pressure Measurement (2009)

Joel handler, Md, is an expert Panel Member of the eighth Joint national committee on High Blood Pressure; Hypertension clinical lead, care Management institute; and Hypertension lead for Southern california Kaiser Permanente, anaheim, ca. e-mail: joel.handler@kp.org. Clinical Scenario A woman, age 72 years, has blood pressures of 150/70 mm Hg and 150/80 mm Hg, obtained by a medical assistant (MA), on consecutive office visits and does not have a history of hypertension. The blood pressure cuff is properly sized, the MA is inquiring about the patient’s last mammogram while obtaining the blood pressure, and the patient is helping to hold her arm up within the MA’s grasp. The mean of a dozen blood pressure readings that the patient has obtained at home is 128/64 mm Hg. Does this patient have white-coat hypertension?
Raw starch-digesting amylase from Chalara paradoxa. (1987)

A black mold strain was isolated from the pith of a sago palm (Metroxylon sagu), in Papua New Guinea. The mold, which was identified as Chalara paradoxa, produced a strong amylase activity, and the enzyme involved hydrolyzed starch granules to glucose. The enzyme showed extremely high activity as to the hydrolysis of starch granules compared with those conventionally studied raw starch-digesting amylases.The culture conditions for production of the enzyme were studied. The optimum culture medium consisted of 4.0% raw sago starch, 6.5% corn steep liquor, 0.7% beef extract, 0.3% NaCl and 0.08% FeSO4·7H2O. The optimum initial pH and optimum temperature were 4.5 and 30°C, respectively.This enzyme was purified by successive starch adsorption, DEAE-cellulose column chromatography and Toyopearl HW-55 gel filtration, the purified enzyme showing 60-fold the activity of the original culture liquor. It gave a single band on disc gel electrophoresis, and the molecular weight estimated on SDS disc gel electrophoresis was 82, 000. On isoelectric focusing, the enzyme showed six bands, at isoelectric points (pl) of 3.20, 3.26, 3.34, 3.41, 3.49 and 3.52.This amylase showed the maximum activity at 45-50°C and pH 5.0. Its pH-stability range was relatively wide. The enzyme retained more than 80% of its initial activity in the pH range of 4.5 to 7.5. It was stable below 50°C, and its thermostability was improved by the addition of calcium ions. The relative reaction rates of the enzyme with various metal ions and inhibitors were also studied. The anomeric configuration of the product obtained from starch with the enzyme was the beta-configuration. The ratio of raw starch-digesting activity to gelatinized starch-saccharifying activity of the enzyme was 31.5%.
BODIPY-Based Fluorescent Probes for Sensing Protein Surface-Hydrophobicity (2015)

Mapping surface hydrophobic interactions in proteins is key to understanding molecular recognition, biological functions, and is central to many protein misfolding diseases. Herein, we report synthesis and application of new BODIPY-based hydrophobic sensors (HPsensors) that are stable and highly fluorescent for pH values ranging from 7.0 to 9.0. Surface hydrophobic measurements of proteins (BSA, apomyoglobin, and myoglobin) by these HPsensors display much stronger signal compared to 8-anilino-1-naphthalene sulfonic acid (ANS), a commonly used hydrophobic probe; HPsensors show a 10- to 60-fold increase in signal strength for the BSA protein with affinity in the nanomolar range. This suggests that these HPsensors can be used as a sensitive indicator of protein surface hydrophobicity. A first principle approach is used to identify the molecular level mechanism for the substantial increase in the fluorescence signal strength. Our results show that conformational change and increased molecular rigidity of the dye due to its hydrophobic interaction with protein lead to fluorescence enhancement.