gebhart/patrepeval huggingface dataset:
Atrial‐like cardiomyocytes from human pluripotent stem cells are a robust preclinical model for assessing atrial‐selective pharmacology (2015)

Drugs targeting atrial-specific ion channels, Kv1.5 or Kir3.1/3.4, are being developed as new therapeutic strategies for atrial fibrillation. However, current preclinical studies carried out in non-cardiac cell lines or animal models may not accurately represent the physiology of a human cardiomyocyte (CM). In the current study, we tested whether human embryonic stem cell (hESC)-derived atrial CMs could predict atrial selectivity of pharmacological compounds. By modulating retinoic acid signaling during hESC differentiation, we generated atrial-like (hESC-atrial) and ventricular-like (hESC-ventricular) CMs. We found the expression of atrial-specific ion channel genes, KCNA5 (encoding Kv1.5) and KCNJ3 (encoding Kir 3.1), in hESC-atrial CMs and further demonstrated that these ion channel genes are regulated by COUP-TF transcription factors. Moreover, in response to multiple ion channel blocker, vernakalant, and Kv1.5 blocker, XEN-D0101, hESC-atrial but not hESC-ventricular CMs showed action potential (AP) prolongation due to a reduction in early repolarization. In hESC-atrial CMs, XEN-R0703, a novel Kir3.1/3.4 blocker restored the AP shortening caused by CCh. Neither CCh nor XEN-R0703 had an effect on hESC-ventricular CMs. In summary, we demonstrate that hESC-atrial CMs are a robust model for pre-clinical testing to assess atrial selectivity of novel antiarrhythmic drugs.
Allergenicity of acid protease secreted by <i>Candida albicans</i> (1996)

We have previously reported the cases of Candida albicans (C. alb) acid protease (CAAP)-induced atopic asthma. In this study, the allergenicity of the released enzyme CAAP was examined among asthmatic patients with positive immediate skin response to crude C. alb antigen. Among 49 patients with positive skin response to crude C. alb, anti-crude C. alb IgE antibodies were detected in 40 and anti-CAAP IgE antibodies were detected in 18. Moreover, anticrude C. alb IgE antibodies were detected in all of the patients in whom anti-CAAP IgE antibodies were detected. No correlations between IgG antibodies to both antigens or between IgE and IgG antibodies to CAAP were observed. CAAP induced significant T-cell proliferation in 20/28 patients showing positive T-cell proliferation response to crude C. alb antigen. Most of the patients showing positive conjunctival response to crude C. alb antigen also showed positive response to CAAP. Most of the patients showing high levels of serum IgE antibody and positive histamine-release response of peripheral blood leukocytes to CAAP showed positive conjunctival response. The results indicate that CAAP is an important allergen in C. alb-related mucosal allergy.
ChemInform Abstract: Polar Hexagonal Tungsten Oxide (HTO) Materials: (1) Synthesis, Characterization, Functional Properties, and Structure—Property Relationships in A<sub>2</sub>(MoO<sub>3</sub>)<sub>3</sub>(SeO<sub>3</sub>) (A: Rb<sup>+</sup> and Tl<sup>+</sup>) and (2) Classification, Structural Distortions, and Second‐Harmonic Generating Properties of Known Polar HTOs. (2010)

Abstract Crystals of the title compounds are prepared from mixtures of MoO 3 , A 2 CO 3 (A: Rb, Tl), SeO 2 , and H 2 O (autoclave, 230 °C, 3 d, 50% and 80% yields for the Rb and Tl compounds, respectively).
Electrophoretic Capture and Detection of Nanoparticles at the Opening of a Membrane Pore Using Scanning Electrochemical Microscopy (2004)

Stochastic electrophoretic capture of individual nanometer-scale particles at the small opening of a conically shaped nanopore in a synthetic membrane is described. Particle capture is sensed using a scanning electrochemical microscope (SECM) to measure the decrease in the transport rate of a redox-active molecule through the pore. The SECM tip is positioned at the larger backside opening of pore and used to amperometrically monitor the transport rate prior, during, and after particle capture. Following capture, the particle is released by electrophoretically driving it out of the pore opening and back into the solution. The capture and release method is demonstrated by detection of charged polystyrene spheres (43-150-nm diameter) using a polycarbonate membrane with conically shaped pores, the small opening of the pore having a diameter of 60 nm. The inverse of the time to capture polystyrene spheres increases with particle concentration over the range 10(8)-10(10) particles/mL. Selective detection based on nanoparticle charge and size is also demonstrated. A quantitative theoretical description of the rate of particle capture is presented, and the physical mechanism of particle capture, based on the balance of electrostatic and entropic forces, is considered.
Characterisation of the binding of [<sup>3</sup>H]‐SB‐674042, a novel nonpeptide antagonist, to the human orexin‐1 receptor (2004)

1. This study characterises the binding of a novel nonpeptide antagonist radioligand, [(3)H]SB-674042 (1-(5-(2-fluoro-phenyl)-2-methyl-thiazol-4-yl)-1-((S)-2-(5-phenyl-(1,3,4)oxadiazol-2-ylmethyl)-pyrrolidin-1-yl)-methanone), to the human orexin-1 (OX(1)) receptor stably expressed in Chinese hamster ovary (CHO) cells in both a whole cell assay and in a cell membrane-based scintillation proximity assay (SPA) format. 2. Specific binding of [(3)H]SB-674042 was saturable in both whole cell and membrane formats. Analyses suggested a single high-affinity site, with K(d) values of 3.76+/-0.45 and 5.03+/-0.31 nm, and corresponding B(max) values of 30.8+/-1.8 and 34.4+/-2.0 pmol mg protein(-1), in whole cell and membrane formats, respectively. Kinetic studies yielded similar K(d) values. 3. Competition studies in whole cells revealed that the native orexin peptides display a low affinity for the OX(1) receptor, with orexin-A displaying a approximately five-fold higher affinity than orexin-B (K(i) values of 318+/-158 and 1516+/-597 nm, respectively). 4. SB-334867, SB-408124 (1-(6,8-difluoro-2-methyl-quinolin-4-yl)-3-(4-dimethylamino-phenyl)-urea) and SB-410220 (1-(5,8-difluoro-quinolin-4-yl)-3-(4-dimethylamino-phenyl)-urea) all displayed high affinity for the OX(1) receptor in both whole cell (K(i) values 99+/-18, 57+/-8.3 and 19+/-4.5 nm, respectively) and membrane (K(i) values 38+/-3.6, 27+/-4.1 and 4.5+/-0.2 nm, respectively) formats. 5. Calcium mobilisation studies showed that SB-334867, SB-408124 and SB-410220 are all functional antagonists of the OX(1) receptor, with potencies in line with their affinities, as measured in the radioligand binding assays, and with approximately 50-fold selectivity over the orexin-2 receptor. 6. These studies indicate that [(3)H]SB-674042 is a specific, high-affinity radioligand for the OX(1) receptor. The availability of this radioligand will be a valuable tool with which to investigate the physiological functions of OX(1) receptors.
B / S Study of Soil- Tea System Based on SSH Integration Technology (2014)

With the support of B /S architecture,MySQL database was operated under JDBC and the soil-tea system was developed on the open- source Eclipse platform. The practical application of Spring,Struts and Hibernate( SSH) in the soil- tea system achieved the light enclosure,database mapping and separation management of 3 business layers. Struts framework based on MVC pattern was employed on presentation layer, Spring on business layer and Hibernate mapping on persistence layer in this paper. This system,including Web services of soil global positioning system( GPS),tea disease- pest diagnosis system and soil nutrient and moisture system,achieved the intelligent management and quantitative decision of informations in soil- tea system. The results showed that the multilayer application system architecture based on SSH integrated framework effectively improved the scalability,maintainability,stability and development efficiency of the system.
Light and electron microscopic study of the distribution of substance P‐immunoreactive fibers and neurokinin‐1 receptors in the skin of the rat lower lip (2001)

Cutaneous antidromic vasodilatation and plasma extravasation, two phenomena that occur in neurogenic inflammation, are partially blocked by substance P (SP) receptor antagonists and are known to be mediated in part by mast cell-released substances, such as histamine, serotonin, and nitric oxide. In an attempt to provide a morphological substrate for the above phenomena, we applied light and electron microscopic immunocytochemistry to investigate the pattern of SP innervation of blood vessels and its relationship to mast cells in the skin of the rat lower lip. Furthermore, we examined the distribution of SP (neurokinin-1) receptors and their relationship to SP-immunoreactive (IR) fibers. Our results confirmed that SP-IR fibers are found in cutaneous nerves and that terminal branches are observed around blood vessels and penetrating the epidermis. SP-IR fibers also innervated hair follicles and sebaceous glands. At the ultrastructural level, SP-IR varicosities were observed adjacent to arterioles, capillaries, venules, and mast cells. The varicosities possessed both dense core vesicles and agranular synaptic vesicles. We quantified the distance between SP-IR varicosities and blood vessel endothelial cells. SP-IR terminals were located within 0.23-5.99 microm from the endothelial cell layer in 82.7% of arterioles, in 90.2% of capillaries, and in 86.9% of venules. Although there was a trend for SP-IR fibers to be located closer to the endothelium of venules, this difference was not significant. Neurokinin-1 receptor (NK-1r) immunoreactivity was most abundant in the upper dermis and was associated with the wall of blood vessels. NK-1r were located in equal amounts on the walls of arterioles, capillaries, and venules that were innervated by SP-IR fibers. The present results favor the concept of a participation of SP in cutaneous neurogenic vasodilatation and plasma extravasation both by an action on blood vessels after binding to the NK-1r and by causing the release of substances from mast cells after diffusion through the connective tissue.
Valuing environmental improvements in the Great Barrier Reef: Ecological and preference heterogeneity in local area case studies (2010)

The focus of this report is to test if protection values at a particular GBR site may be easily transferred to other case studies of interest in the region. The research involved valuing three local case studies in the GBR and testing how values were consistent across site and population characteristics. The sites were chosen to reflect substantial heterogeneity in extent, ecological composition and condition, while values were assessed for both local and distant populations. The results are encouraging, indicating that although significant heterogeneity was identified with the mixed logit models, values were robust to various site and population differences. No significant difference in protection values between the three local case studies could be identified, and there was no significant difference in values between the local population and the Brisbane population. However, some evidence for distance effects was identified for the Brisbane population, with closer sites valued more highly. As well, potential losses were valued more highly (in absolute terms) than potential gains. The implication of these results is that protection values are likely to be higher for closer reef areas with risks of losses than these with opportunities for improvements.
3-D-Printed Wideband Multi-Ring Dielectric Resonator Antenna (2019)

A three-dimensional (3-D)-printed wideband multi-ring dielectric resonator antenna (DRA) is investigated for the first time. It consists of four concentric dielectric rings with decreasing effective dielectric constants in the radial direction. To facilitate the 3-D-printed design, a basic unit cubic cell that can be easily adjusted to provide different effective dielectric constants is used. An axial coaxial probe is used to excite the DRA in three transverse magnetic (TM) modes (TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">01δ</sub> , TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">02δ</sub> , and TM <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">03δ</sub> ), which are merged to provide a wide impedance bandwidth. For demonstration, a prototype operating in C-band is designed and fabricated using a 3-D printer. Its reflection coefficient, radiation pattern, antenna gain, and antenna efficiency are measured, and reasonable agreement between the measured and simulated results is observed. The prototype has a wide measured 10-dB impedance bandwidth of 60.2% (4.3-8.0 GHz) with a low profile of 0.18λ <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">0</sub> , where λ0 is the wavelength in air at the center frequency (6.15 GHz). The prototype has an average measured antenna efficiency of 89%, with the peak efficiency given by 95%.
Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood (2011)

Cryopreservation of hematopoietic stem cells (HSCs) and hematopoietic progenitor cells (HPCs) is crucial for cord blood (CB) banking and transplantation. We evaluated recovery of functional HPC cryopreserved as mononuclear or unseparated cells for up to 23.5 years compared with prefreeze values of the same CB units. Highly efficient recovery (80%-100%) was apparent for granulocyte-macrophage and multipotential hematopoietic progenitors, although some collections had reproducible low recovery. Proliferative potential, response to multiple cytokines, and replating of HPC colonies was extensive. CD34(+) cells isolated from CB cryopreserved for up to 21 years had long-term (≥ 6 month) engrafting capability in primary and secondary immunodeficient mice reflecting recovery of long-term repopulating, self-renewing HSCs. We recovered functionally responsive CD4(+) and CD8(+) T lymphocytes, generated induced pluripotent stem (iPS) cells with differentiation representing all 3 germ cell lineages in vitro and in vivo, and detected high proliferative endothelial colony forming cells, results of relevance to CB biology and banking.
Power-Aware Processor Scheduling under Average Delay Constraints (2005)

In this paper, voltage scaling strategies for scheduling aperiodic tasks under average delay constraints are studied. Dynamic voltage scaling in single processor systems is formulated as a constrained stochastic optimization problem for which the optimal solution can be obtained using a combination of Lagrange relaxation and the value iteration method. For multiprocessor systems, we present a two-phase approach. In the first phase, the speed settings and static workload distribution of the processors are optimized to minimize the total power dissipation. Dynamic voltage scaling techniques are then applied to each individual processor in the second phase. Both homogeneous and heterogeneous systems have been investigated. Based on queueing theory, the proposed algorithms guarantee conformity to the average delay constraint. Moreover, our simulation experiments have shown they are effective for minimizing power consumption.
Amortized efficiency of list update and paging rules (1985)

In this article we study the amortized efficiency of the “move-to-front” and similar rules for dynamically maintaining a linear list. Under the assumption that accessing the ith element from the front of the list takes θ(i) time, we show that move-to-front is within a constant factor of optimum among a wide class of list maintenance rules. Other natural heuristics, such as the transpose and frequency count rules, do not share this property. We generalize our results to show that move-to-front is within a constant factor of optimum as long as the access cost is a convex function. We also study paging, a setting in which the access cost is not convex. The paging rule corresponding to move-to-front is the “least recently used” (LRU) replacement rule. We analyze the amortized complexity of LRU, showing that its efficiency differs from that of the off-line paging rule (Belady's MIN algorithm) by a factor that depends on the size of fast memory. No on-line paging algorithm has better amortized performance.
100-Channel wireless neural recording system with 54-Mb/s data link and 40%-efficiency power link (2012)

For real-time monitoring of brain activities, a highdata-rate, low-power, and highly mobile neural recording system is desirable. This paper presents a complete chipset for a 100-channel wireless neural recording system, which consists of 3 ICs — a neural interface (NI) IC and a wireless power RX and data TX IC for an implant unit (IU), and a wireless data RX IC for an external head unit (EHU). With a dual S/H NI architecture and a burst-mode (BM) wideband (WB) FSK TX, the IU achieves a 100-channel recording and wireless transmission at 54.24Mb/s while consuming only 6.6mW. Using power coupling with optimal resonant load transformation and high-efficiency rectifier and LDO circuits, the whole wireless power link achieves 40% efficiency over 1cm distance with 0.5cm tissue in between. The EHU needs to transmit the RF power lower than 30mW to operate the IU. The EHU is implemented using a crystal-less BM WB FSK RX consuming only 14.4mW at 27.12Mb/s.
Cellular response in naphthalene-induced Clara cell injury and bronchiolar epithelial repair in mice (1995)

Clara cells, progenitors for bronchiolar epithelium, are also primary targets for metabolically activated pulmonary cytotoxicants and have an abundance of the cytochrome P-450 monooxygenases required for xenobiotic metabolism. To define the repair pattern after massive Clara cell injury, mice were treated with naphthalene, and lungs evaluated 1-14 days postinjury (DPI). Clara cells of terminal bronchioles were vacuolated and swollen 1 DPI, exfoliated 2 DPI, and resembled controls at 14 DPI. The volume fraction of vacuolated cells was highest 1 and 2 DPI and minimal at 5-7 DPI. The volume fraction of normal nonciliated cells decreased 40% at 1 DPI. Cell proliferation increased within epithelium and interstitium at 1 DPI, was maximal at 2 DPI, and at all other time points was similar to baseline levels. Expression of Clara cell differentiation markers was barely detectable in terminal bronchiolar epithelium at 1 and 2 DPI, clearly detectable at 4 DPI, and gradually returned to control levels at 5-14 DPI. We conclude that bronchiolar epithelial repair after naphthalene injury involves distinct phases of proliferation and differentiation, proliferation of cells that are not differentiated Clara cells, and interaction of multiple cell types including nontarget cells.
Fast-scanning two-photon fluorescence imaging using a microelectromechanical systems (MEMS) two-dimensional scanning mirror (2006)

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce laser-scanning instrumentation based on a microelectromechanical systems (MEMS) scanner and describe two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.5 kHz. (180.2520) Fluorescence microscopy (180.5810) Scanning microscopy
Oscillations in giant magnetoresistance and antiferromagnetic coupling in [Ni81Fe19/Cu]<i>N</i> multilayers (1992)

We report giant magnetoresistance in [Ni81Fe19/Cu]N multilayers. Saturation magnetoresistance values exceeding 16% for saturation fields of only 600 Oe are found at 300 K. In addition, we show evidence for well-defined oscillations in saturation magnetoresistance as a function of Cu spacer layer thickness at 4.2 K, with an oscillation period and phase similar to that in Co/Cu multilayers. However, the temperature dependence of the magnetoresistance, while weak for thin Cu layers, is much stronger for thicker Cu layers. Consequently at 300 K only a single oscillation in magnetoresistance for thin Cu layers is found. We show that the properties of the Ni81Fe19/Cu multilayers are very sensitive to annealing at moderate temperatures, which may limit the possible technological applications of such structures.
Epigenetic dysregulation of K<sub>Ca</sub>3.1 channels induces poor prognosis in lung cancer (2015)

Epigenomic changes are an important feature of malignant tumors. How tumor aggressiveness is affected by DNA methylation of specific loci is largely unexplored. In genome-wide DNA methylation analyses, we identified the KCa 3.1 channel gene (KCNN4) promoter to be hypomethylated in an aggressive non-small-cell lung carcinoma (NSCLC) cell line and in patient samples. Accordingly, KCa 3.1 expression was increased in more aggressive NSCLC cells. Both findings were strong predictors for poor prognosis in lung adenocarcinoma. Increased KCa 3.1 expression was associated with aggressive features of NSCLC cells. Proliferation and migration of pro-metastatic NSCLC cells depended on KCa 3.1 activity. Mechanistically, elevated KCa 3.1 expression hyperpolarized the membrane potential, thereby augmenting the driving force for Ca(2+) influx. KCa 3.1 blockade strongly reduced the growth of xenografted NSCLC cells in mice as measured by positron emission tomography-computed tomography. Thus, loss of DNA methylation of the KCNN4 promoter and increased KCa 3.1 channel expression and function are mechanistically linked to poor survival of NSCLC patients.
CD-SEM precision: improved procedure and analysis (1999)

Accurate precision assessment becomes increasingly important as we proceed along the SIA road map, in to more advanced processes and smaller critical dimensions. Accurate precision is necessary in order to determine the P/T ratio which is used to decide whether a specific CD-SEM is valid for controlling a specific process. The customer's needs, as been presented by the SEMATECH Advanced Metrology Advisory Group, are to receive a detailed precision report, in the form of a full repeatability and reproducibility (RR) analysis. The 3 sigma single tool RR, of an in-line SEM, are determined in the same operational modes as used in production, and should include the effects of time and process variants on the SEM performance. We hereby present an RR procedure by a modulate approach which enables the user extending the evaluation according to her/his needs. It includes direct assessment of repeatability, reproducibility and stability analysis. It also allows for a study of wafer non homogeneity, induced process variation and a measured feature type effect on precision. The procedure is based on the standard ISO RR procedure, and includes a modification for a correct compensation for bias, or so called measurement turned. A close examination of the repeatability and reproducibility variations, provides insight to the possible sources of those variations, such as S/N ratio, SEM autofocus mechanism, automation etc. For example, poor wafer alignment might not effect the repeatability, but severally reduce reproducibility. Therefore the analysis is a key to better understanding and improving of CD-SEM performance, on production layers. The procedure is fully implemented on an automated CD-SEM, providing on line precision assessment. RR < 1 nm has been demonstrated on well defined resist and etched structures. Examples of the automatic analysis results, using the new procedure are presented.