gebhart/patrepeval huggingface dataset:
Identification and Characterization of <i>Drosophila</i> Relatives of the Yeast Transcriptional Activator SNF2/SWI2 (1994)

The Drosophila brahma (brm) gene encodes an activator of homeotic genes that is highly related to the yeast transcriptional activator SWI2 (SNF2), a potential helicase. To determine whether brm is a functional homolog of SWI2 or merely a member of a family of SWI2-related genes, we searched for additional Drosophila genes related to SWI2 and examined their function in yeast cells. In addition to brm, we identified one other Drosophila relative of SWI2: the closely related ISWI gene. The 1,027-residue ISWI protein contains the DNA-dependent ATPase domain characteristic of the SWI2 protein family but lacks the three other domains common to brm and SWI2. In contrast, the ISWI protein is highly related (70% identical) to the human hSNF2L protein over its entire length, suggesting that they may be functional homologs. The DNA-dependent ATPase domains of brm and SWI2, but not ISWI, are functionally interchangeable; a chimeric SWI2-brm protein partially rescued the slow growth of swi2- cells and supported transcriptional activation mediated by the glucocorticoid receptor in vivo in yeast cells. These findings indicate that brm is the closest Drosophila relative of SWI2 and suggest that brm and SWI2 play similar roles in transcriptional activation.
Comparison of three cell block techniques for detection of low frequency abnormal cells (2013)

Background: The Cellient ® Automated Cell Block System rapidly creates paraffin-embedded cell blocks by using vacuum filtration to deposit a layer of cells on a filter and infiltrate those cells with reagents and paraffin. This study used a “tracer” cell model to mimic low frequency abnormal cells and compare detection and representative sampling with simple sedimentation, Richard-Allan HistoGel™, and Cellient cell block techniques. Methods: Tracer cells were a cultured cell line (CaSki) fixed in methanol, prestained in solution with hematoxylin, and quantified using a hemacytometer. Tracer cells were diluted in a 10-fold dilution series ranging from 100 to 0.1 tracer/mL in a background of pooled clinical serous effusion specimens. Ten replicates of each dilution were processed using each cell block method, and the resulting blocks were cut to produce two slides from each block. The slides were deparaffinized, counterstained with eosin, cover-slipped, and screened for the presence of tracer cells. Blocks were considered to be representative of the specimen if tracer cells were detected on either of the slides. If no tracer cells were observed on either slide, the block was recut to generate a third slide. If tracer cells were seen on the third slide, the block was considered representative of the specimen. Results: Tracer cells were identified on the initial slides for 20 of 40 (50.0%) simple sedimentation, 21 of 40 (52.5%) of HistoGel, and 25 of 40 (62.5%) of Cellient cell blocks. Representative sampling of the 1 tracer/mL specimen was 10.0% for simple sedimentation and 30.0% for HistoGel and Cellient. Only Cellient showed representative sampling of the 0.1 tracer/mL specimen. Conclusion: The Cellient System blocks demonstrated representative sampling at the lowest tracer cell concentration compared with simple sedimentation and HistoGel. Keywords: Cellient ® , HistoGel™, simple sedimentation, CaSki, microtomy
Dimorphecolic Acid—A Unique Hydroxydienoid Fatty Acid<sup>2</sup> (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDimorphecolic Acid—A Unique Hydroxydienoid Fatty Acid2C. R. Smith Jr., T. L. Wilson, E. H. Melvin, and I. A. WolffCite this: J. Am. Chem. Soc. 1960, 82, 6, 1417–1421Publication Date (Print):March 1, 1960Publication History Published online1 May 2002Published inissue 1 March 1960https://pubs.acs.org/doi/10.1021/ja01491a034https://doi.org/10.1021/ja01491a034research-articleACS PublicationsRequest reuse permissionsArticle Views179Altmetric-Citations93LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development and validation of a new MRI simulation technique that can reliably estimate optimal in vivo scanning parameters in a glioblastoma murine model (2018)

Scanning parameter optimization using the computer simulation was shown to be a valid surrogate to the in vivo approach in a glioblastoma murine model yielding in a better delineation and differentiation of the tumor from the contralateral hemisphere. In addition to drastically reducing the time invested in choosing optimal scanning parameters when compared to an in vivo approach, this simulation program could also be used to harmonize MRI acquisition parameters across scanners from different vendors.
Purification and Properties of the Escherichia coli Deoxyribonucleic Acid-unwinding Protein (1974)

Abstract The DNA-unwinding protein from Escherichia coli has been purified to homogeneity. It is a single polypeptide of 22,000 daltons; the native molecular weight is 90,000. The effect of the protein on the activity of the three DNA polymerases of E. coli has been studied. The activities of DNA polymerases I and III are significantly reduced, whereas DNA polymerase II activity is enhanced in the presence of unwinding protein. The rate of transcription catalyzed by E. coli RNA polymerase in the presence of the protein is reduced when single-stranded, but not double-stranded, DNAs are employed as templates. Using fd DNA as template in a DNA synthetic reaction that is dependent on both RNA polymerase and DNA polymerase II, the unwinding protein was found to be essential for the synthesis of a DNA product that is equal in size to the template. With the use of crude cell-free extracts of E. coli, it was shown that DNA polymerase II can convert bacteriophage fd DNA to the replicative form. These experiments suggest a possible physiological role for DNA polymerase II and the unwinding protein of E. coli.
Cytokine‐inducible SH2 protein‐3 (CIS3/SOCS3) inhibits <i>Janus</i> tyrosine kinase by binding through the N‐terminal kinase inhibitory region as well as SH2 domain (1999)

CIS3 inhibits JAK2 kinase activity by binding to the activation loop through the SH2 domain, and KIR is necessary for kinase inhibition. Although the inhibitory mechanism by CIS3 is similar to that by JAB, the contributions of the SH2 domain and KIR for binding are different between JAB and CIS3. Our study defined the inhibitory mechanism of CIS3 and provides a useful information for creating a novel tyrosine kinase inhibitor.
SunnyTALEN: A second‐generation TALEN system for human genome editing (2013)

Transcription activator-like effector nucleases (TALENs) have rapidly emerged as a powerful genome editing tool. The site-specific DNA double-strand breaks generated by TALENs in the human chromosome can induce homologous recombination or non-homologous end joining, resulting in desired genetic modifications. In this study, we report the development of a TALEN variant, SunnyTALEN, with >2.5-fold improved genome editing efficacy in human cells. The corresponding scaffold increases the rate of genetic modification at all the 13 tested loci of human genome and is compatible with heterodimer TALEN architectures. This enhanced and high-efficiency TALEN variant represents a novel second-generation TALEN system and has great potential for biological and therapeutic applications.
Die Entkoppelung von Produktions- und Arbeitsprozeß (1987)

Zusammenfassung Verschiedene empirische Studien über die Einführung und Nutzung der CNC-Technologie haben gezeigt, daß bei gleichen oder zumindest ähnlichen technischen und ökonomischen Rahmenbedingungen arbeitsorganisatorisch verschieden verfahren wird. Der gegebene Organisationsspielraum wird theoretisch als Resultat einer zunehmenden Entkoppelung von Produktions- und Arbeitsprozeß, einzelne Organisationsvarianten werden als Lösungsversuche einer strukturellen Problematik von Prozeß- und Arbeitsökonomie begriffen. Die objektive Problematik der Organisationsbildung ist ihrerseits in soziale Kräftekonstellationen eingebunden, die selbst wiederum auf das organisationsstrukturelle Problem von Fremdsteuerung und Eigenregulierung von Arbeit verweisen.
An automata-theoretic approach to modular model checking (2000)

In modular verification the specification of a module consists of two part. One part describes the guaranteed behavior of the module. The other part describes the assumed behavior of the system in which the module is interacting. This is called the assume-guarantee paradigm. In this paper we consider assume-guarantee specifications in which the guarantee is specified by branching temporal formulas. We distinguish between two approaches. In the first approach, the assumption is specified by branching temporal formulas too. In the second approach, the assumption is specified by linear temporal logic. We consider guarantees in ∀CTL, and ∀CTL*. We develop two fundamental techniques: building maximal models for ∀CTL and ∀CTL* formulas and using alternating automata to obtain space-efficient algorithms for fair model checking. Using these techniques we classify the complexity of satisfiability, validity, implication, and modular verification for ∀CTL and ∀CTL*. We show that modular verification is PSPACE-complete for ∀CTL and is EXSPACE-complete for ∀CTL*. We prove that when the assumption is linear, these bounds hold also for guarantees in CTL and CTL*. On the other hand, the problem remains EXSPACE-hard even when we restrict the assumptions to LTL and take the guarantees as a fixed ∀CTL formula.
A Simple Activity Measurement of Lysozyme (1971)

Journal Article A Simple Activity Measurement of Lysozyme Get access Taiji Imoto, Taiji Imoto Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi Search for other works by this author on: Oxford Academic Google Scholar Kazuyoshi Yagishita Kazuyoshi Yagishita Department of Agricultural Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi Search for other works by this author on: Oxford Academic Google Scholar Agricultural and Biological Chemistry, Volume 35, Issue 7, 1 July 1971, Pages 1154–1156, https://doi.org/10.1080/00021369.1971.10860050 Published: 01 July 1971 Article history Received: 24 April 1971 Published: 01 July 1971
A Near Real-Time System for Security Assurance Assessment (2008)

Building systems that are guaranteed to be secure or to remain secure over time is still an unachievable goal. The need for a tool that helps to determine security assurance level of a system is therefore vital in order to maintain and improve overall security. This paper introduces our system to assess the overall security assurance of a large, networked, IT-driven system in terms of a dedicated evaluation infrastructure based on multi-agent technology. We use attack graph approach to compute an attackability metric value and define other metrics for anomaly detection to assess both the static and dynamic visions of the system under study. The implemented software system is described, and the examples of experiments for evaluating of network component, sub network and network security assurance levels are considered.
Sugar formulation effect on available lysine content of dulce de leche (1999)

Dulce de leche is a dairy-based confectionery product, widely consumed in Argentina and other Latin American countries as filling or topping for desserts, cakes and biscuits (Hansen, 1978; Bouzas, 1997). It is prepared by heat concentration of whole milk with added sucrose until it reaches 700 g total solids/kg dulce de leche. Sucrose is usually partly replaced by other sugars (not more than 400 g/kg of total sugars added according to Normas Mercosur, 1996) to avoid crystallization. Sodium bicarbonate is added to prevent coagulation of proteins as the pH of the mixture decreases and the isoelectric pH of proteins increases during heating. Maillard browning is the main reaction of milk proteins during the manufacture of dulce de leche. This results in an attractive flavour and colour, but also leads to less desirable changes, such as loss of nutritional value and formation of toxic compounds (Rizzi, 1994; Friedman, 1996 a ). The natural reactants are free amino groups of milk proteins and lactose. However, as the addition of other reducing sugars is an accepted practice, the consequences may be quite different depending on the formulation used. In addition, other reactions leading to crosslinkages between protein chains contribute to the reduction in nutritional value (Mauron, 1990; Friedman, 1996 b ; Finot, 1997). The reduction of nutritional quality of milk proteins is attributed to the lower digestibility and the biological inactivation or destruction of essential amino acids. Lysine is the essential amino acid most affected as its free ε-amino group is very reactive (Hurrell, 1990; Mauron, 1990; Finot, 1997). Several studies have investigated the physical and rheological characteristics (Pauletti et al . 1984, 1996), sensory properties (Sabioni et al . 1984; Buera et al . 1990; Hough et al . 1990), sugar composition and microbiological stability (Ferramondo et al . 1984) of dulce de leche. However, there is little information about the loss in available lysine during manufacture (Pavlovic et al . 1994). The aim of this study was to examine the effects of the nature and quantity of various sugars commonly used in the manufacture of dulce de leche on the loss of available lysine.
Mechanisms of photosensitization induced by drugs: A general survey (2000)

This paper presents a general survey of the mechanisms involved in drug phototoxicity. Moreover, a list of 174currently used clinical drugs inducing photosensitization is provided in addition to some others from which phototoxiceffects are suspected. Likewise, some aspects related to the mechanisms involved in the phototoxicity of fluoroquinolonesand non steroidal-antiinflammatory drugs have been reviewed. Finally, a possible role of the arenediazonium ionsas photosensitisers is discussed.
Time-of-Flight-Based Radio Tomography for Device Free Localization (2013)

Due to its ability of realizing device free localization with wireless networks, the radio tomography becomes a promising technique that draws considerable attention. Traditional radio tomography makes use of the received signal strength (RSS) of wireless links to realize location estimation. However, the RSS measurement is particularly sensitive to noise. Inspired by the fact that similar to the RSS, the time-of-flight (TOF) measurement also changes significantly when some objects shadow the wireless link, and the fact that compared with the RSS, the TOF measurement is robust to noise, a novel TOF-based radio tomography is proposed in this paper. With the TOF measurements of the shadowed links as observation information, a modified particle filter algorithm which utilizes the compressive sensing technique to produce the importance distribution of the particle set is proposed, so as to realize localization and tracking with under-sampled measurements by making full use of the space-domain sparse and time-domain gradually changed feature of the location information. The experiments with the 802.15.4a chirp spread spectrum ranging hardware are presented to confirm the proposed scheme.
[Diagnosis and surgical methods of managing patients with chronic abdominal ischaemia]. (2010)

Presented herein are the findings of examination of sixty-two patients with clinical manifestations of chronic abdominal ischaemia predominantly induced by stenosis of the celiac trunk. The authors carried out a comparative analysis of efficacy of instrumental methods of diagnosis of chronic abdominal ischaemia syndrome, having also investigated the lesions of the visceral branches by the findings of angiography. Also presented herein are the outcomes of various methods of surgical correction: 47 reconstructive and conditionally reconstructive operations, 10 transcutaneous endovascular angioplasties, also considering complications of the immediate postoperative period as well as short- and long-term outcomes of surgical treatment.
Effects of barium on stimulus‐induced rises of [K<sup>+</sup>]<sub>o</sub> in human epileptic non‐sclerotic and sclerotic hippocampal area CA1 (2000)

In the hippocampus of patients with therapy-refractory temporal lobe epilepsy, glial cells of area CA1 might be less able to take up potassium ions via barium-sensitive inwardly rectifying and voltage-independent potassium channels. Using ion-selective microelectrodes we investigated the effects of barium on rises in [K+]o induced by repetitive alvear stimulation in slices from surgically removed hippocampi with and without Ammon's horn sclerosis (AHS and non-AHS). In non-AHS tissue, barium augmented rises in [K+]o by 147% and prolonged the half time of recovery by 90%. The barium effect was reversible, concentration dependent, and persisted in the presence of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), N-methyl-D-aspartate (NMDA) and gamma-aminobutyric acid [GABA(A)] receptor antagonists. In AHS tissue, barium caused a decrease in the baseline level of [K+]o. In contrast to non-AHS slices, in AHS slices with intact synaptic transmission, barium had no effect on the stimulus-induced rises of [K+]o, and the half time of recovery from the rise was less prolonged (by 57%). Under conditions of blocked synaptic transmission, barium augmented stimulus-induced rises in [K+]o, but only by 40%. In both tissues, barium significantly reduced negative slow-field potentials following repetitive stimulation but did not alter the mean population spike amplitude. The findings suggest a significant contribution of glial barium-sensitive K+-channels to K+-buffering in non-AHS tissue and an impairment of glial barium-sensitive K+-uptake in AHS tissue.
New aspects of Saccharomyces cerevisiae as a novel carrier for berberine (2013)

Analytical methods proved that berberine was encapsulated in the yeast cells. Fluorescence microscopy and FTIR results showed the entrance of berberine inside the yeasts. DSC diagram indicated the appearance of new peak which is due to the synthesis of new product. Although plasmolysis caused changes in yeast cell structure and properties, it did not enhance berberine loading in the cells. The results confirmed that Saccharomyces cerevisiae could be an efficient and safe carrier for active materials.
Coupling endoplasmic reticulum stress to the cell death program: role of the ER chaperone GRP78 (2002)

Alterations in Ca(2+) homeostasis and accumulation of unfolded proteins in the endoplasmic reticulum (ER) lead to an ER stress response. Prolonged ER stress may lead to cell death. Glucose-regulated protein (GRP) 78 (Bip) is an ER lumen protein whose expression is induced during ER stress. GRP78 is involved in polypeptide translocation across the ER membrane, and also acts as an apoptotic regulator by protecting the host cell against ER stress-induced cell death, although the mechanism by which GRP78 exerts its cytoprotective effect is not understood. The present study was carried out to determine whether one of the mechanisms of cell death inhibition by GRP78 involves inhibition of caspase activation. Our studies indicate that treatment of cells with ER stress inducers causes GRP78 to redistribute from the ER lumen with subpopulations existing in the cytosol and as an ER transmembrane protein. GRP78 inhibits cytochrome c-mediated caspase activation in a cell-free system, and expression of GRP78 blocks both caspase activation and caspase-mediated cell death. GRP78 forms a complex with caspase-7 and -12 and prevents release of caspase-12 from the ER. Addition of (d)ATP dissociates this complex and may facilitate movement of caspase-12 into the cytoplasm to set in motion the cytosolic component of the ER stress-induced apoptotic cascade. These results define a novel protective role for GRP78 in preventing ER stress-induced cell death.
Measurement of surface topography of magnetic recording materials through computer analyzed microscopic interferometry (1983)

A recently developed surface characterization technique, Computer Analyzed Microscopic Interferometry, is shown to have many applications in the study of magnetic recording surfaces. Three-dimensional and quantitative interferometric images produce very high depth resolution without reducing the field of view. This allows very precise surface roughness and volume measurements.
The role of neutrophil apoptosis in juvenile‐onset systemic lupus erythematosus (2009)

Our data demonstrate an imbalance in proapoptotic and antiapoptotic factors in both neutrophils and sera from patients with juvenile-onset SLE. This imbalance results in increased neutrophil apoptosis in these patients. Correlations with markers of disease activity indicate that altered neutrophil apoptosis in juvenile-onset SLE patients may play a pathogenic role in this condition.
[The effects of diazoxide on myocardium function and the expressions of ERK and JNK in isolated spontaneous hypertension rat hearts]. (2006)

These results indicated that diazoxide preconditioning could mimic ischemic preconditioning, the activation of ERK expression and the declining of JNK expression involved in diazoxide preconditioning in isolated SHR hearts.
Activated nitriles in heterocyclic synthesis. Synthesis of several new pyrimidine and pyridazine derivatives (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTActivated nitriles in heterocyclic synthesis. Synthesis of several new pyrimidine and pyridazine derivativesKamal Usef Sadek, Sherif Mahmoud Fahmy, Rafat Mild Mohareb, and Mohamed Hilmy ElnagdiCite this: J. Chem. Eng. Data 1984, 29, 1, 101–103Publication Date (Print):January 1, 1984Publication History Published online1 May 2002Published inissue 1 January 1984https://pubs.acs.org/doi/10.1021/je00035a033https://doi.org/10.1021/je00035a033research-articleACS PublicationsRequest reuse permissionsArticle Views136Altmetric-Citations19LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts