gebhart/patrepeval huggingface dataset:
Monte Carlo Shielding Calculations for a Spent Fuel Transport Cask with Automated Monte Carlo Variance Reduction (2011)

For the purpose of performing reasonable shielding calculation of a spent fuel transport cask, the use of Monte Carlo methods has been proposed for solving the radiation transport problem on a detailed structure of the transport cask considering fixed neutron sources. A SMIRE (Simplified MCNP-ANISN_W Variance Reduction) system has been developed in the present study, which is possible to generate automatically the lower weight boundary of the weight window for each mesh based on the Consistent Adjoint Driven Importance Sampling (CADIS). Compared with the case of the importance based on the empirical formula, the figure of merit is increased by a factor of 25. In this system, it is possible to calculate the weight suitable for the distantly-positioned detector point from the fuel effective region to introduce the relaxation factor which relaxes the increase of the particle numbers at the boundary of weight window meshes that are generally caused by the large attenuation of adjoint flux. This system is used for a variety of the radiation transport problems as well as the transport cask.
CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms (2019)

CRISPR-Cas9-based genome editing has transformed the life sciences, enabling virtually unlimited genetic manipulation of genomes: The RNA-guided Cas9 endonuclease cuts DNA at a specific target sequence and the resulting double-strand breaks are mended by one of the intrinsic cellular repair pathways. Imprecise double-strand repair will introduce random mutations such as indels or point mutations, whereas precise editing will restore or specifically edit the locus as mandated by an endogenous or exogenously provided template. Recent studies indicate that CRISPR-induced DNA cuts may also result in the exchange of genetic information between homologous chromosome arms. However, conclusive data of such recombination events in higher eukaryotes are lacking. Here, we show that in <i>Drosophila</i>, the detected Cas9-mediated editing events frequently resulted in germline-transmitted exchange of chromosome arms-often without indels. These findings demonstrate the feasibility of using the system for generating recombinants and also highlight an unforeseen risk of using CRISPR-Cas9 for therapeutic intervention.
Control of a brushless DC motor/generator in a fuel cell hybrid electric vehicle (2009)

In this paper, the control of a brushless DC motor/generator in a light motorcycle powered by fuel cell energy for mass production is presented. The zero emission vehicle development will improve air quality and protect the environment. The control strategies for motoring and regenerative braking operations of a brushless DC motor are explained in detail based on a system hybrid control of a fuel cell hybrid vehicle. A prototype experimental test-bed is built for experimental verifications and results are discussed.
Robust traffic design for dynamic routing networks (1991)

The authors present traffic restoration design algorithms to attain a robust network for any facility link or node failure. Some of these algorithms were implemented on network models to compute the cost for doing traffic restoration for different traffic restoration level objectives. From their simulation studies, the authors found that with efficient use of trunk group diversity and at a reasonable incremental cost, one can obtain a robust network to respond to a major facility link or a node failure and still meet the network objectives. A similar approach based on the algorithms presented in this work has been proposed for design of the worldwide intelligent network.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Biofilm Formation by <i>Staphylococcus epidermidis</i> Depends on Functional RsbU, an Activator of the <i>sigB</i> Operon: Differential Activation Mechanisms Due to Ethanol and Salt Stress (2001)

Staphylococcus epidermidis is a common pathogen in medical device-associated infections. Its major pathogenetic factor is the ability to form adherent biofilms. The polysaccharide intercellular adhesin (PIA), which is synthesized by the products of the icaADBC gene cluster, is essential for biofilm accumulation. In the present study, we characterized the gene locus inactivated by Tn917 insertions of two isogenic, icaADBC-independent, biofilm-negative mutants, M15 and M19, of the biofilm-producing bacterium S. epidermidis 1457. The insertion site was the same in both of the mutants and was located in the first gene, rsbU, of an operon highly homologous to the sigB operons of Staphylococcus aureus and Bacillus subtilis. Supplementation of Trypticase soy broth with NaCl (TSB(NaCl)) or ethanol (TSB(EtOH)), both of which are known activators of sigB, led to increased biofilm formation and PIA synthesis by S. epidermidis 1457. Insertion of Tn917 into rsbU, a positive regulator of alternative sigma factor sigma(B), led to a biofilm-negative phenotype and almost undetectable PIA production. Interestingly, in TSB(EtOH), the mutants were enabled to form a biofilm again with phenotypes similar to those of the wild type. In TSB(NaCl), the mutants still displayed a biofilm-negative phenotype. No difference in primary attachment between the mutants and the wild type was observed. Similar phenotypic changes were observed after transfer of the Tn917 insertion of mutant M15 to the independent and biofilm-producing strain S. epidermidis 8400. In 11 clinical S. epidermidis strains, a restriction fragment length polymorphism of the sigB operon was detected which was independent of the presence of the icaADBC locus and a biofilm-positive phenotype. Obviously, different mechanisms are operative in the regulation of PIA expression in stationary phase and under stress induced by salt or ethanol.
A Comparative Analysis of the Sugar Phosphate Cyclase Superfamily Involved in Primary and Secondary Metabolism (2006)

Sugar phosphate cyclases (SPCs) catalyze the cyclization of sugar phosphates to produce a variety of cyclitol intermediates that serve as the building blocks of many primary metabolites, for example, aromatic amino acids, and clinically relevant secondary metabolites, for example, aminocyclitol/aminoglycoside and ansamycin antibiotics. Feeding experiments with isotopically labeled cyclitols revealed that cetoniacytone A, a unique C(7)N-aminocyclitol antibiotic isolated from an insect endophytic Actinomyces sp., is derived from 2-epi-5-epi-valiolone, a product of SPC. By using heterologous probes from the 2-epi-5-epi-valiolone synthase class of SPCs, an SPC homologue gene, cetA, was isolated from the cetoniacytone producer. cetA is closely related to BE-orf9 found in the BE-40644 biosynthetic gene cluster from Actinoplanes sp. strain A40644. Recombinant expression of cetA and BE-orf9 and biochemical characterization of the gene products confirmed their function as 2-epi-5-epi-valiolone synthases. Further phylogenetic analysis of SPC sequences revealed a new clade of SPCs that might regulate the biosynthesis of a novel set of secondary metabolites.
Upstream Differencing for Multiphase Flow in Reservoir Simulation (1991)

Upstream differencing for multiphase flow in reservoir simulation is analyzed. The associated numerical flux is shown to be well defined, monotone, Lipschitz–continuous, and consistent. In the case of a two-phase flow the corresponding numerical scheme is convergent and the numerical flux is compared to that of Godunov and Engquist–Osher. Finally, a simple way to obtain a higher-order scheme is outlined.
Hormonal regulation of malic enzyme and glucose-6-phosphate dehydrogenase in brown adipose tissue (1993)

The activities of malic enzyme (ME) and glucose-6-phosphate dehydrogenase (G-6-PDH), two NADPH-generating lipogenic enzymes, were measured in brown adipose tissue (BAT) of rats undergoing various neurohormonal manipulations. Methimazole-induced hypothyroidism doubled the activity of these two enzymes but, surprisingly, triiodothyronine (T3) given to hypothyroid rats caused a time- and dose-dependent stimulation of up to three- to fourfold. Unilateral BAT denervation modestly reduced the activity of these enzymes (approximately 30%) and failed to prevent the stimulation induced by hypothyroidism, whereas growth hormone (GH) successfully blocked this effect of hypothyroidism. Insulin stimulated both enzymes regardless of the thyroid status but failed to abolish the inhibitory effect of GH. In intact rats, cold exposure caused a time-dependent increase in the activity of both ME and G-6-PDH, which reached 5.2- and 3-fold, respectively, after 96 h. This cold-induced stimulation was not observed in hypothyroid rats, but it was restored by physiological doses of thyroxine (800 ng.100 g body wt-1.24 h-1). Replacement with T3 (300 ng.100 g body wt-1.24 h-1), in contrast, did not have this effect. In hypothyroid rats with hemidenervation of BAT, norepinephrine (NE) modestly increased ME and G-6-PDH activities in the denervated side, with little or no effect in the intact side. Receptor-saturating doses of T3 (50 micrograms.100 g body wt-1.day-1 over 48 h) stimulated two- and threefold both enzymes in both sides, reducing or obliterating the effect of denervation. The data suggest a complex neurohormonal regulation of the activity of ME and G-6-PDH in BAT.(ABSTRACT TRUNCATED AT 250 WORDS)
Essential Role of the Thymus to Reconstitute Naive (CD45RA+) T-Helper Cells After Human Allogeneic Bone Marrow Transplantation (1997)

To contribute to the understanding of the role of the thymus in humans in the reconstitution of naive (CD45RA+) T cells after bone marrow transplantation (BMT), we compared T-cell regeneration in a unique situation, namely a thymectomized cancer patient (15 years old), with that of thymus-bearing patients after allogeneic BMT. These cases shared features of transplantation (total body irradiation, HLA-matched donors, and graft-versus-host disease prophylaxis with cyclosporine A) and all had an uncomplicated posttransplantation course. As shown by fluorescence-activated cell sorting analyses, the thymectomized host failed to reconstitute CD45RA+ T-helper cells even 24 months after BMT (11% CD45RA+ of CD4+ cells). In this patient, preferentially CD45RO+ cells contributed to the recovery of CD4+ cells (206 of 261/μL at 6 months and 463 of 558/μL at 24 months after BMT, CD45RA+ of CD4+ cells), whereas CD45RA+ cells remained low (<60/μL). In contrast, nine thymus-bearing hosts (5 children and 4 adults) examined between 6 and 24 months after BMT effectively reconstituted CD4+/CD45RA+ cells according to their normal age-related range (≥28% in adults and ≥50% in children). Five of these were analyzed sequentially at 6 and 9 months after BMT. Within this period, CD45RA+ cells increasingly contributed to the recovery of CD4+ cells (median, +21%), even when total CD4+ cells decreased. With respect to T-cytotoxic/suppressor cells, the thymectomized host retained the capacity to recover CD45RA+ cells (137 of 333/μL at 6 months and 596 of 1,046/μL at 24 months after BMT, CD45RA+ of CD8+ cells), a proportion similar to that seen in thymus-bearing hosts. These findings suggest that a thymus-independent pathway exists to regenerate CD45RA+ T-cytotoxic/suppressor cells, but residual thymus is essential to reconstitute naive (CD45RA+) T-helper cells after BMT in humans.
<title>Automated rendezvous and capture system development and simulation for NASA</title> (2004)

The United States does not have an Automated Rendezvous and Capture/Docking (AR&C) capability and is reliant on manned control for rendezvous and docking of orbiting spacecraft. This reliance on the labor intensive manned interface for control of rendezvous and docking vehicles has a significant impact on the cost of the operation of the International Space Station (ISS) and precludes the use of any U.S. expendable launch capabilities for Space Station resupply. The Marshall Space Flight Center (MSFC) has conducted pioneering research in the development of an automated rendezvous and capture (or docking) (AR&C) system for U.S. space vehicles. This AR&C system was tested extensively using hardware-in-the-loop simulations in the Flight Robotics Laboratory, and a rendezvous sensor, the Video Guidance Sensor was developed and successfully flown on the Space Shuttle on flights STS-87 and STS-95, proving the concept of a video- based sensor. Further developments in sensor technology and vehicle and target configuration have lead to continued improvements and changes in AR&C system development and simulation. A new Advanced Video Guidance Sensor (AVGS) with target will be utilized as the primary navigation sensor on the Demonstration of Autonomous Rendezvous Technologies (DART) flight experiment in 2004. Realtime closed-loop simulations will be performed to validate the improved AR&C systems prior to flight.
Inhibition of K transport by divalent cations in sickle erythrocytes (1987)

We report experiments on the effect of intracellular divalent cations (Mg, Ca, Mn) on K transport and cell volume in erythrocytes from patients with homozygous hemoglobin S disease (SS cells). When CO-treated SS erythrocytes are exposed to the ionophore A23187, removal of cell Mg markedly stimulates K efflux, whereas increasing cell Mg inhibits K efflux. The Ki for the inhibition by internal free Mg is 0.38 +/- 0.10 mmol/L, a value comparable to the concentration of free Mg in normal cells (0.3 to 0.4 mmol/L). When swollen SS cells with increased Mg content were incubated in plasma-like medium, they shrunk much less than swollen SS cells with normal Mg content. Thus, elevation of cell Mg produces inhibition of swelling-induced K movement from SS cells. Internal Ca and Mn also inhibit K movement from SS cells. The inhibition of volume regulation by divalent cations suggests that increases in intracellular divalent ions, especially Mg, could induce a persistent degree of cell swelling in SS RBCs and thereby inhibit intracellular polymerization.
Generalizability of Scores Influenced by Multiple Sources of Variance (1965)

Generalizability theory concerns the adequacy with which a “universe” score can be inferred from a set of observations. In this paper the theory is applied to a universe in which observations are classifiable according to two independent variable aspects of the measuring procedure. Several types of universe scores are developed and the variance components ascertained for each type. The composition of expected observed-score variance and the adequacy of inference to a particular type of universe score is a function of the procedure used in gathering data. A generalizability study provides estimates of variance components which can be used in designing an efficient procedure for a particular decision purpose.
Compte-rendu de Lucie DUPRÉ, Du marron à la châtaigne. La relance d'un produit régional, Paris, Éditions du Comité des travaux historiques et scientifiques, 2002, 334 p. (2002)

http://ruralia.revues.org/document322.html
State-dependent block of voltage-gated Na+ channels by amitriptyline via the local anesthetic receptor and its implication for neuropathic pain (2004)

Amitriptyline is a tricyclic antidepressant, which also alleviates various pain syndromes at its therapeutic plasma concentration (0.36-0.90 microM). Accumulated evidence suggests that such efficacy may be due to block of voltage-gated Na(+) channels. The Na(+) channel alpha-subunit protein consists of four homologous domains (D1-D4), each with six transmembrane segments (S1-S6). The aims of this study were to locate the amitriptyline receptor in the Na(+) channel alpha-subunit and to compare the amitriptyline affinity in open, inactivated, and resting states of the Na(+) channel. Wild-type and mutant rat skeletal muscle alpha-subunit Na(+) channels were expressed in human embryonic kidney cells and assayed under whole-cell voltage clamp conditions. Our results indicate that the amitriptyline receptor overlaps with the local anesthetic receptor to a great extent in Na(+) channels. Residues N434 (at D1-S6), L1280 (D3-S6), and F1579 (D4-S6) may jointly form parts of the amitriptyline/local anesthetic receptor, with residue L1280 being most critical for amitriptyline binding. Open-channel block by amitriptyline was assessed in inactivation-deficient Na(+) channels and compared with the resting- and inactivated-channel block in wild-type channels. The open-channel block by amitriptyline has the highest affinity, with a 50% inhibitory concentration (IC(50)) of 0.26 microM. The inactivated-channel block by amitriptyline had a weaker affinity (0.51 microM), whereas the resting-channel displayed the weakest affinity (33 microM). We hypothesize that selective block of both persistent late openings and the inactivated state of neuronal Na(+) channel isoforms by amitriptyline also occurs at its therapeutic concentration and likely contributes to its efficacy in pain syndromes.
Fabrication of directed nano wire networks through self-assembly (2004)

We present a major development in our novel, self-assembly-based nano wire fabrication method. When it was first reported in MEMS 2003, the method could successfully produce a network of nano wires that are 50 nm in width and hundreds of microns long despite little control on the global shape of the network itself. However, the recent development enables us to impose very complicated shapes on the nano wire network by assigning points of wire initiation and termination by means of simple lithography. Hence, 1) the number, and 2) the direction of nano wires can be dictated.
<i>Ganoderma lucidum</i> Polysaccharides: Immunomodulation and Potential Anti-Tumor Activities (2011)

Ganoderma lucidum (G. lucidum), a basidiomycete white rot fungus, has long been prescribed to prevent and treat various human diseases, particularly in China, Japan, and Korea. Several classes of bioactive substances have been isolated and identified from G. lucidum, such as triterpenoids, polysaccharides, nucleosides, sterols, and alkaloids, among others. This paper examines the potential role of G. lucidum polysaccharide (GLPS) in tumor therapy and the possible mechanisms involved. Both in vitro and in vivo studies suggested that the anti-tumor activities of GLPS are mediated by its immunomodulatory, anti-angiogenic, and cytotoxic effects. GLPS affects immune cells and immune-related cells including B lymphocytes, T lymphocytes, dendritic cells, macrophages, and natural killer cells. In addition, recent data also suggest that GLPS suppresses tumorigenesis or inhibits tumor growth through direct cytotoxic effect and anti-angiogenic actions. However, many questions still need to be answered before both G. lucidum and GLPS can be widely accepted and used as anti-tumor agents.
A Case Study of School-Based Science Curriculum Development: Overview of Project Approach and Process of Implementation (2002)

Under the impetus of the Hong Kong education reform, curriculum development has brought a whole new range of perspectives with an emphasis on Learning to Learn. Schools are encouraged to formulate their own curriculum development plan according to their individual situation and readiness. (Curriculum Development Council, 2001a). This School-Based Science Curriculum Development (SBSCD) project was established to echo the need for professional guidance and support in this
A novel sialylfucopentaose in human milk. Presence of this oligosaccharide is not dependent on expression of the secretor or Lewis fucosyltransferases. (1987)

We have identified a novel oligosaccharide in human milk that is a fucosyl derivative of sialyltetrasaccharide c (NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc). This oligosaccharide was purified by affinity chromatography on a column of immobilized Ricinus communis I lectin. Structural analyses of radiolabeled oligosaccharides by exoglycosidase digestions, binding by specific anti-carbohydrate antibodies, and analysis of the 3H-labeled glucitol derivative obtained after permethylation and hydrolysis are consistent with the following proposed structure. (formula; see text) The analyses of human milk sialylpentasaccharides from donors typed as Le(a-,b+), Le(a+,b-), and Le(a-,b-) secretor confirmed the secretor gene-dependent expression of the sialylated lacto-N-fucopentaose I (Fuc alpha 1-2Gal beta 1-3[NeuAc alpha 2-6]GlcNAc beta 1-3Gal beta 1-4Glc) and the Lewis gene-dependent expression of the sialylated lacto-N-fucopentaose II (NeuAc alpha 2-3Gal beta 1-3[Fuc alpha 1-4]GlcNAc beta 1-4Glc). However, the presence of this novel oligosaccharide in human milk is not dependent on the expression of either the secretor gene or the Lewis gene-specified fucosyltransferases.
A Preliminary Study of Aflatoxin B1 Detection in Peanut Oil by Terahertz Time-Domain Spectroscopy (2014)

<abstract> <sc>Abstract.</sc> Aflatoxins are fungal secondary toxic metabolites that can cause serious health problems in animals and humans. Terahertz (THz) spectroscopy is promising as a nondestructive analytical tool for chemical and biochemical detection. In this technical note, THz time-domain spectroscopy (THz-TDS) was used for the quantitative analysis of aflatoxin B1 (AFB1) in peanut oil. Chemometrics methods including partial least squares regression (PLSR) and stepwise multiple linear regression (SMLR) were used to build quantitative models. Their performance was compared using two spectral pretreatment methods (first and second derivative). The results show that the SMLR model was more stable than the PLSR model, although its prediction precision was slightly lower. When 1.11 and 0.72 THz frequencies were used, the root mean square errors of calibration (RMSEC) and cross-validation (RMSECV) were 18.2 and 27.7 ppm, respectively, and the corresponding correlation coefficients (r<sub>c</sub> and r<sub>cv</sub>) were 0.9639 and 0.9309, respectively. The overall results indicate that THz-TDS combined with frequency selection could be applied as a rapid and nondestructive tool for the determination of AFB1 content in peanut oil.
Shoots from Douglas-fir cultures (1977)

Whole Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seed embryos were placed on defined medium containing 0.05 or 0.1 mg/ℓ BAP (benzylaminopurine). One vigorous shoot usually grew from the tip of each swollen cotyledon on the 0.05 BAP medium, but small multiple shoots were produced on the 0.1 BAP medium. After transfer of embryos or excised cotyledons to medium without hormones, the vigorous shoots developed normally but the small multiple shoots did not. However, when cotyledons were grown on medium containing auxin and cytokinin, callus was produced that was isolated and subcultured monthly to fresh medium. Shoots were produced from subcultured cotyledon callus in 11 of 35 cultures, for a frequency of 31%. Two of 30–50 excised shoots rooted after treatment with 10 mg/ℓ IBA (indolebutyric acid). A few shoots were also produced from subcultured needle callus and from stem explant callus from young seedling material.