gebhart/patrepeval huggingface dataset:
Clinical analysis of acute pulmonary embolism with type 2 diabetes mellitus (2016)

Objective
To explore the clinical features and recurrence rates of acute pulmonary embolism (APE) with type 2 diabetes mellitus(DM2).


Methods
126 patients with acute pulmonary embolism were retrospectively analysed from January 2010 to December 2015. All patients were divided into two groups:observation group (APE with DM2,43 cases) and control group(APE without DM2,83 cases).The differences of clinical manifestations, complications,laboratory results,thrombus localizations,risk stratification and the recurrence rates of acute pulmonary embolism were analysed between the two groups.


Results
There were no significant differences in age,sex and clinical manifestations between the two groups. The combination of respiratory failure,coronary heart disease and pneumonia were more frequently seen in observation group(P<0.05). The level of creatine kinase, lactate dehydrogenase, fasting blood glucose,cTnI,NT-pro-BNP, CREA were obviously higher than control group(P<0.05). A higher risk stratification and recurrence rate were observed in the bservation group(P<0.05).


Conclusion
Patients with acute pulmonary embolism and type 2 diabetes mellitus are more serious and more affected by complications and a higher recurrence rate,thus,we should give more attention to these patients and reduce the recurrence rate.


Key words: 
Pulmonary embolism; Type 2 diabetes mellitus; Recurrence rate
Linear-Grating Triboelectric Generator Based on Sliding Electrification (2013)

The triboelectric effect is known for many centuries and it is the cause of many charging phenomena. However, it has not been utilized for energy harvesting until very recently. (1-5) Here we developed a new principle of triboelectric generator (TEG) based on a fully contacted, sliding electrification process, which lays a fundamentally new mechanism for designing universal, high-performance TEGs to harvest diverse forms of mechanical energy in our daily life. Relative displacement between two sliding surfaces of opposite triboelectric polarities generates uncompensated surface triboelectric charges; the corresponding polarization created a voltage drop that results in a flow of induced electrons between electrodes. Grating of linear rows on the sliding surfaces enables substantial enhancements of total charges, output current, and current frequency. The TEG was demonstrated to be an efficient power source for simultaneously driving a number of small electronics. The principle established in this work can be applied to TEGs of different configurations that accommodate the needs of harvesting energy and/or sensing from diverse mechanical motions, such as contacted sliding, lateral translation, and rotation/rolling.
Degradation of Aromatics and Chloroaromatics by<i>Pseudomonas</i>sp. Strain B13: Cloning, Characterization, and Analysis of Sequences Encoding 3-Oxoadipate:Succinyl-Coenzyme A (CoA) Transferase and 3-Oxoadipyl-CoA Thiolase (2002)

3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase carry out the ultimate steps in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates in bacteria utilizing the 3-oxoadipate pathway. This report describes the characterization of DNA fragments with the overall length of 5.9 kb from Pseudomonas sp. strain B13 that encode these enzymes. DNA sequence analysis revealed five open reading frames (ORFs) plus an incomplete one. ORF1, of unknown function, has a length of 414 bp. ORF2 (catI) encodes a polypeptide of 282 amino acids and starts at nucleotide 813. ORF3 (catJ) encodes a polypeptide of 260 amino acids and begins at nucleotide 1661. CatI and CatJ are the subunits of the 3-oxoadipate:succinyl-CoA transferase, whose activity was demonstrated when both genes were ligated into expression vector pET11a. ORF4, termed catF, codes for a protein of 401 amino acid residues with a predicted mass of 41,678 Da with 3-oxoadipyl-CoA thiolase activity. The last three ORFs seem to form an operon since they are oriented in the same direction and showed an overlapping of 1 bp between catI and catJ and of 4 bp between catJ and catF. Conserved functional groups important for the catalytic activity of CoA transferases and thiolases were identified in CatI, CatJ, and CatF. ORF5 (catD) encodes the 3-oxoadipate enol-lactone hydrolase. An incomplete ORF6 of 1,183 bp downstream of ORF5 and oriented in the opposite direction was found. The protein sequence deduced from ORF6 showed a putative AMP-binding domain signature.
[Flow cytometric analysis of the effect of DWA2114R on the cell cycle traverse of RPMI 8402 cells--comparison with CDDP]. (1992)

The (-) - (R) - 2-aminomethylpyrrolidine (1, 1-cyclobutanedicarboxylato) platinum(II) monohydrate, DW A2114R, is a new derivative of cis-dichlorodiammineplatinum(II), CDDP, which is reported to have a lower nephrotoxicity than CDDP. In this report, we investigated the effects of DW A2114R, in comparison with CDDP, on the cell growth, the cell cycle traverse and the colony-forming ability of human leukemia RPMI 8402 cells in vitro. The inhibitory effects of DW A2114R on the cell growth and the colony-forming ability were about 1/10 and 1/10-1/30, respectively, of the CDDP dose. And DW A2114R showed a higher time-dependency than CDDP in the experiment comparing exposure time to the drugs. Analysis of DNA histograms obtained by flow cytometry revealed that DW A2114R had a similar effect on the cell cycle traverse to that of CDDP. Both drugs exerted their growth-inhibitory effect by blocking cells at G2 phase. At higher concentrations, they prolonged the traverse of cells through S phase and completely inhibited them throughout the cell cycle. The comparative study on the colony-forming ability suggested that the G2 phase accumulation was irreversible and that these drugs are lethal, so the G2 phase accumulation could be considered a cytocidal effect of the drugs. In early clinical studies, however, the dose of DW A2114R was put at about 15-fold higher than the therapeutic dose of CDDP. This was comparable with our results in vitro and DW A2114R showed a much lower toxicities, with special regard to nephrotoxicity, than CD DP. These clinical data and our results suggest that DW A2114R could be clinically effective as an antitumor agent.
Design approaches for digitally dominated active pixel sensors: leveraging Moore's Law scaling in focal plane readout design (2008)

Although CMOS technology scaling has provided tremendous power and circuit density benefits for innumerable applications, focal plane array (FPA) readouts have largely been left behind due to dynamic range and signal-to-noise considerations. However, if an appropriate pixel front end can be constructed to interface with a mostly digital pixel, it is possible to develop sensor architectures for which performance scales favorably with advancing technology nodes. Although the front-end design must be optimized to interface with a particular detector, the dominant back end architecture provides considerable potential for design reuse. In this work, digitally dominated long wave infrared (LWIR) active pixel sensors with cutoff wavelengths between 9 and 14.5 μm are demonstrated. Two ROIC designs are discussed, each fabricated in a 90-nm digital CMOS process and implementing a 256 x 256 pixel array on a 30-μm pitch. In one of the implemented designs, the feasibility of implementing a 15-μm pixel pitch FPA with a 500 million electron effective well depth, less than 0.5% non-linearity in the target range and a measured NEdT of less than 50 mK at f/4 and 60 K is demonstrated. Simple on-FPA signal processing allows for a much reduced readout bandwidth requirement with these architectures. To demonstrate the potential for commonality that is offered by a digitally dominated architecture, this LWIR sensor design is compared and contrasted with other digital focal plane architectures. Opportunities and challenges for application of this approach to various detector technologies, optical wavelength ranges and systems are discussed.
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter (2004)

The sterol regulatory element binding-proteins (SREBPs) are transcription factors that regulate the genes of lipid metabolism. Cholesterol and unsaturated fatty acids regulate SREBPs. Giardia lamblia (GL) is an intestinal parasite and one of the earliest derived members within the eukaryotic lineage. GLs exist as trophozoites and cysts. Growth in cholesterol depletion induces transcription of cyst-wall protein (CWP) genes that are upregulated during encystation. The hypothesis was investigated that SREBP-like pathways have a role in cwp gene transcription. Chinese hamster ovary cells were transfected with a cwp-2 promoter reporter construct. Incubation with cholesterol or oleate reduced cwp-2 mediated gene transcription to about half of the control. Incubation in sterol-depleted media, or in the presence of either an inhibitor of intracellular cholesterol movement or inhibitor of cholesterol synthesis, increased gene expression up to 3-fold. Overexpression of SREBPs increased reporter gene activity 2.5-fold. In the absence of functional SREBPs, cwp-2 was not regulated by cholesterol. Footprint analysis of cwp-2 reveals three novel binding sites for mammalian SREBPs with no homologies in other species or humans. The data show that SREBP binds to and can modulate transcription of a regulatory element from an ancient eukaryote and suggest the existence of an SREBP homolog in GL.
Project Aedes transgenic population control in Juazeiro and Jacobina Bahia, Brazil (2014)

The genetic control of insects is a promising technology to be applied for the control of wild populations in urban and agricultural areas as an alternative to the widely used methods based on chemical and mechanical control. In Brazil, there is considerable concern about the occurrence of Dengue disease across the country, and a clear demand for improved vector control. A joint project among Medfly and Mosquito Facility Brazil, University of Sao Paulo and Oxitec was agreed upon in order to test in urban areas, social, technical and operational aspects of genetic control for A. aegypti population employing an RIDL transgenic strain. The PAT - Project Aedes Transgenic - was established in the city of Juazeiro, by a demand of Secretary of Health of State of Bahia. As the first large project on releasing transgenic insets in a human populated area, the PAT would follow all phases and steps recommended by the international community and fit into the strict Brazilian biosecurity laws.

Community engagement - From the outset the project partners have worked closely with the Brazilian regulatory system to obtain required permits for field activities. It has been a clear focus of PAT from the outset to adopt full transparency and a vigorous and proactive community engagement campaign.

Mass rearing - Critical to any SIT program is the ability to consistently rear large volumes of high quality insects. Egg production has an average of 12 million/week and 700,000 males. Field activities have concentrated on the suburb of Itaberaba. Males were marked with different colors and released from the same point in order to evaluate relative dispersal and longevity. The RIDL strain dispersed further with a mean distance travel of 75m (95% CI 30-109) compared to wild Itaberaba strain, 44m (95% CI 20-73), although this difference was not significant. Ovitrapping formed the main monitoring tool for A. aegypti - wild populations in both the treated and the untreated areas of Itaberaba. Larvae hatched from field-collected eggs from ovitraps were screened for fluorescence to determine paternity (fluorescent larvae, transgenic father; non-fluorescent larvae, wild-type father). Adult trapping was conducted periodically using aspiration surveys and BG-traps. This coincided with the release of marked cohorts of RIDL mosquitoes enabling longevity of released males to be assessed. After 12 months of twice week releases - 18 millions - the suppression reached 96%. In another district - Mandacaru - the releases started in march 2012 and with the density of 14,000 males/ha/week in 5 months, 100% of larva captured in ovitraps were fluorescent, indicating a high suppression. After the success in both districts, a larger target was aimed, a urban population of 45,000 people, city of Jacobina, where the releases started in June 2013 with a perspective to reach a large suppression in 24 months.
Effects of the temperature, the duration of frozen storage, and the freezing container on in vitro measurements in human peripheral blood mononuclear cells (1996)

PBMCs isolated by leukapheresis and ficoll-hypaque treatment can be frozen with 10-percent DMSO in a -80 degrees C mechanical freezer. When a PVC bag is used for freezing and storage of PBMCs at -80 degrees C, the duration of frozen storage should not exceed 1.5 years, whereas PBMCs frozen in a polyolefin bag can be stored in a -135 degrees C freezer for as long as 2.4 years. When these guidelines were followed, in vitro recovery was 90 percent that of fresh PBMCs, viability was 90 percent, and growth in the CFU-GEMM tissue culture assay was similar to that of fresh PBMCs. The PBMCs frozen and stored in PVC or polyolefin bags exhibited satisfactory results, whereas those stored in cryostorage vials did not.
眼镜王蛇多肽OH-CATH30可作为潜在候选药物分子杀死临床耐药菌 (2018)

Cationic antimicrobial peptides (AMPs) are considered as important candidate therapeutic agents, which exert potent microbicidal properties against bacteria, fungi and some viruses. Based on our previous findings king cobra cathelicidin (OH-CATH) is a 34-amino acid peptide that exerts strong antibacterial and weak hemolytic activity. The aim of this research is to evaluate the efficacy of both OH-CATH30 and its analog D-OH-CATH30 against clinical isolates comparing with routinely utilized antibiotics in vitro. In this study, 584 clinical isolates were tested (spanning 2013-2016) and the efficacy of the candidate peptides and antibiotics were determined by a broth microdilution method according to the CLSI guidelines. Among the 584 clinical isolates, 85% were susceptible to OH-CATH30 and its analogs. Both L- and D-OH-CATH30 showed higher efficacy against (toward) Gram-positive bacteria and stronger antibacterial activity against nearly all Gram-negative bacteria tested compare with antibiotics. The highest bactericidal activity was detected against Acinetobacter spp., including multi-drug-resistant Acinetobacter baumannii (MRAB) and methicillin-resistant Staphylococcus aureus (MRSA). The overall efficacy of OH-CATH30 and its analogs was higher than that of the 9 routinely used antibiotics. OH-CATH30 is a promising candidate drug for the treatment of a wide variety of bacterial infections which are resistant to many routinely used antimicrobial agents.
An Electrical Hot-Wire Inclinometer (1919)

The hot-wire inclinometer consists essentially of two fine platinum wires (diameter about 0.1 mm.) mounted parallel to one another in a closed chamber at a distance apart equal to about 1 mm. A constant current of from 0.4 to 1.5 ampere (according to the sensitivity desired) is maintained in the wires, which constitute two of the arms of a Wheatstone bridge arranged so that the bridge is balanced in the zero position of the inclinometer. If desired, the bridge may be constituted of two pairs of heated wires as above. The inclinometer wires, in the zero position, may be either vertical or horizontal or inclined at any angle to the horizontal. The indications of the instrument are dependent upon the relative heating or cooling effects experienced by the respective wires when the orientation of the wires with regard to a hozontal plane is altered. The galvanoter deflection occurring with alteration in such orientation serves to indicate the inclination of a definitive radius vector to the horizontal, and calibration curves are given for the cases where the axis of rotation is horizontal, and (a) in the plane of the wires and paralel to the wires, and (b) at right angles to the plane of the wires. The characteristics of the calibration curves are discussed and curves are given showing how the resistances of the respective wires depend upon the orientation of the wires with regard to the horizontal plane referred to. Attention is directed to the finite width of the column of heated gas ascending from the wires, and an empirical formula is deduced expressing the maximum deflection as a function of the bridge current employed.
Selective and Rapid O-Acylation of Hydroxyl Groups of Nucleosides By Means of Phase Transfer Catalysis (1993)

Abstract Hydroxyl functions of various thymidine and undine derivatives could be selectively and rapidly benzoylated without modification of the imide groups by means of phase transfer catalysis. the selectivity and reaction rate were highly dependent on the kind and concentration of alkali.
Fabrication of functionally gradient TiN coatings by reactive plasma spraying. (1996)

The nitriding process of solid state Ti-alloy by RF plasma was fundamentally investigated. Based on the results obtained in the nitriding process, reactive plasma spraying of Ti powder was carried out to fabricate the thick nitride coatings. The wear resistance of the nitride coatings was also evaluated by the conventional wear test.The results obtained are summarized as follows:(1) A linear relationship was recognized in the relation between thickness of the nitride layer and square root of nitride time. Therefore, the thermal plasma process seemed to be a diffusion dominating process.(2) The diffusion rate of nitrogen in the Ti-alloy: Rd was estimated from the experimenal results obtained, and the plasma spraying of Ti powder was conducted under the deposition rate of less than Rd. By using this process, it was possible to fabricate the nitride coating with high deposition rate.(3) It revealed that the nitriding degree of the coating could be changed by controlling the coating deposition rate. Based on this results, functionally gradient coating, whose compositional configuration changed from coating/substrate interface to coating surface, was fabricated by controlling the deposition rate.(4) From the results of wear tests, it was found that the wear resistance property of the nitride coating obtained was equivalent to that of nitride layer on the Ti-alloy.
Toxicity of liposomal 3'-5'-O-dipalmitoyl-5-fluoro-2'-deoxyuridine in mice. (1998)

Toxicities of 5-fluoro-2'-deoxyuridine (FUdR) and its liposome incorporated dipalmitoyl derivative (FUdR-dipalmitate) to mouse bone marrow, spleen, liver and ileum were compared after treatment for 6 consecutive days. The applied doses of the two formulations, which were shown earlier to have equal antitumor activity in mouse tumor models, were 600 and 2 mumol/kg respectively. When applied in these doses, toxicity to the hemopoietic system, measured as a decreases in progenitor and precursor cells of the erythroid and granuloid/macrophage lineage in bone marrow and spleen, was more severe for FUdR than for liposomal FUdR-dipalmitate. In the liver, mitotic figures, as indicators of cell division, were absent for both drugs while in control livers the number of cells in mitosis was approximately 2%. Toxicity to the ileum was more severe for liposomal FUdR-dipalmitate than for FUdR and was manifested by granulocyte infiltration, the presence of cell debris, loss of columnar epithelial cells and enlarged nuclei with prominent nucleoli in these cells. Thus, by prolonging the retention time of FUdR in vivo, using liposomes as a vehicle and FUdR-dipalmitate as a lipophilic prodrug, the dose-limiting toxicity appears to shift from bone marrow to the gastrointestinal tract.
Studies on canine pregnancy using B‐mode ultrasound: Development of the conceptus and determination of gestational age (1990)

ABSTRACT Development of the conceptus was characterised using B‐mode ultrasound in a standard size of dog (labrador retrievers, golden retrievers and their crosses; weight range 22 to 30 kg). Growth curves were established for various measurements of the conceptuses. Regression equations allowed the prediction of the expected time of parturition. Multiple regression analysis of two measurements (biparietal diameter and trunk diameter) resulted in an increase in the confidence of prediction.
Five New Legionella Species Isolated from Water (1993)

Fourteen Legionella-like strains isolated from aquatic sources have been characterized serologically, biochemically, and in terms of DNA relatedness. The strains grew on buffered charcoal-yeast extract agar but not on blood agar and displayed phenotypic characteristics typical of the family Legionellaceae, including a requirement for cysteine, cellular fatty acid compositions in which branched-chain acids predominate, and the possession of isoprenoid quinones of the ubiquinone series with more than 10 isoprene units in their side chains. All were nonfermentative, lacked urease, were incapable of nitrate reduction, and reacted positively with a DNA probe specific for the Legionellaceae. DNA hybridization studies in which the hydroxyapatite method was used demonstrated that the strains represented five new species of the genus Legionella. Nine of the strains were more than 90% interrelated, and the name Legionella londiniensis sp. nov. is proposed for this group. Two strains formed a second hybridization group, for which the name Legionella nautarum sp. nov. is proposed, while the three remaining species, Legionella geestiana sp. nov., Legionella quateirensis sp. nov., and Legionella worsleiensis sp. nov., are each represented by a single strain. The levels of relatedness of the new species to each other are 23% or less, and the levels of relatedness to other members of the genus ranged from 0 to 36%. L. geestiana, L. nautarum, and L. londiniensis are serologically unrelated to all other known Legionella species. L. worsleiensis cannot be separated from Legionella pneumophila serogroup 4 by serological methods and is also serologically indistinguishable from L. quateirensis; distinctions may be made on the basis of fatty acid composition and biochemical reactions.
An adaptive thin film transistor (1966)

A thin film transistor (TFT) is described and demonstrated whose transfer characteristic can be reversibly adapted by a short duration voltage pulse applied to a high impedance gate electrode. The device is a four terminal-two gate structure: A source, drain, and insulator gate contact form the basic TFT while the amount of polarity of the polarization charge on the surface of the ferroelectric material of a second gate contact determines the pinch-off voltage of the TFT transfer characteristic. The characteristic is adaptive because once the polarization charge has been changed by an applied potential to the ferroelectric gate contact it will remain at its new value even if the potential is removed. The time required to adapt the characteristic is limited by the switching time of the ferroelectric material which, in general, can be of the order of microseconds. Experimental adaptive thin film transistors have been fabricated using triglycine sulfate for the ferroelectric (single crystal wafer used as substrate), evaporated silicon monoxide for the insulator and evaporated tellurium (p-type) for the semiconductor material. Preliminary electrical measurements indicate that the pinch-off voltage is adaptable over a sizable range and as a consequence, TFT transconductance ratios in excess of 100 to 1 are possible for maximum positive and negative polarization states of the ferroelectric material.