gebhart/patrepeval huggingface dataset:
Evolution of Organic Aerosols in the Atmosphere (2009)

Organic aerosol (OA) particles affect climate forcing and human health, but their sources and evolution remain poorly characterized. We present a unifying model framework describing the atmospheric evolution of OA that is constrained by high-time-resolution measurements of its composition, volatility, and oxidation state. OA and OA precursor gases evolve by becoming increasingly oxidized, less volatile, and more hygroscopic, leading to the formation of oxygenated organic aerosol (OOA), with concentrations comparable to those of sulfate aerosol throughout the Northern Hemisphere. Our model framework captures the dynamic aging behavior observed in both the atmosphere and laboratory: It can serve as a basis for improving parameterizations in regional and global models.
Binding phenomena of isolated unique plasmic degradation products of human cross-linked fibrin. (1979)

Proteolysis of human cross-linked fibrin by plasmin results in the formation of a DD . E complex, and Fragments DD and E as the major degradation products. Three species of Fragment E, which differ both in molecular weights (E1, Mr = 60,000; E2, Mr = 55,000; E3, Mr = 50,000) and in charge, have been isolated from a digest of cross-linked fibrin. Each Fragment E species reacts with monospecific anti-E antiserum. Fragments E1 and E2 bind with Fragment DD to form a DD . E complex but Fragment E3 is inactive. This binding is specific since these Fragments E do not bind to fibrinogen or to degradation products of fibrinogen or of noncross-linked fibrin. Fragments E1 and E2 incubated with plasmin are degraded to Fragment E3, suggesting that the three species represent sequential degradation products. Plasmin-treated Fragments E1 and E2 no longer bind with Fragment DD; therefore, it appears that the peptides cleaved from Fragment E2 by plasmin contain or modify the sites responsible for complex formation. On the other hand, Fragment DD binds not only to Fragments E1 and E2, but also to fibrinogen, Fragments X (Stage 1), X (Stage 2), Y, and NH2-terminal disulfide knot, but only after thrombin treatment, suggesting that Fragment DD binds to complementary sites on the NH2-terminal region of fibrinogen which are exposed after thrombin treatment.
Filamin Binds to the Cytoplasmic Domain of the β1-Integrin (1998)

Integrins play an important role in regulating cell adhesion, motility, and activation. In an effort to identify intracellular proteins expressed by activated T cells that interact with the cytoplasmic domain of beta1-integrin (CD29), we used the beta1-integrin cytoplasmic domain as bait in the yeast two-hybrid system. Here we report that the cytoplasmic domain of beta1-integrin specifically interacts with the cytoskeletal protein filamin. This interaction required all but the most carboxyl-terminal three residues of the cytoplasmic domain of beta1, and the carboxyl-terminal 477 residues of filamin containing the terminal 4. 5 approximately 96-residue tandem repeats of filamin. To verify this interaction in vivo, we showed that filamin specifically coprecipitated with beta1 in mammalian cells. We also showed that recombinant filamin chimeric proteins were able to bind to the beta1 cytoplasmic domain in vitro. We observed that a subset of single point mutations in the cytoplasmic domain of beta1, which had been previously reported to impair its function, disrupt the interaction between beta1 and filamin. Taken together, these findings suggest that the interaction between beta1 and filamin, which in turn can bind actin, provides a mechanism for the interaction of this cell surface receptor with cytoskeletal proteins and that this interaction plays a role in normal receptor function.
Estimation of Twelve Bacopa Saponins in Bacopa monnieri Extracts and Formulations by High-Performance Liquid Chromatography (2006)

A simple and sensitive reversed phase high performance liquid chromatographic (HPLC) method has been developed for the simultaneous determination of twelve bacopa saponins present in the extracts of the Indian Medicinal Plant, Bacopa monnieri. The separation was achieved on a reversed phase C(18) column (Luna C(18)), 5 microm by isocratic elution with 0.05 M sodium sulphate buffer (pH 2.3) and acetonitrile (68.5 : 31.5, v/v) as the mobile phase at a flow rate of 1.0 ml/min with an operating temperature of 30 degrees C. The method was validated for linearity, precision, intra- and inter-day precision and accuracy. Several Bacopa samples (plant materials, extracts and commercial formulations) were successfully analyzed. Major bacopasaponins were bacosides A(3) (3), bacopaside II (4), bacopaside I (5), bacopaside X (6), bacopasaponin C (7), bacopaside N2 (9) and the minor components were bacopasaponin F (1), bacopasaponin E (2), bacopaside N1 (8) bacopaside III (10), bacopaside IV (11) and bacopaside V (12). The total saponin content in the samples, plant materials and extracts varied from 5.1 to 22.17% and 1.47 to 66.03 mg/capsule or tablet in the commercial formulations.
Analysis and visualization of properties of free-form surfaces (2002)

The purposes of this research are to analyze and visualize some properties of aesthetic surfaces and to construct the shapes, which satisfy designers' intentions. To extract and visualize the properties, surface edges of a free-form surface were introduced. The edges are loci of the points that have the extremum values for characteristics of the surfaces such as curvature, height, brightness and so on. By analyzing idea sketches drawn by styling designers, we find that the designers' intentions are closely related to the contour curves and the surface edges. The results of this research give fundamental technology not only to classify features of free-form surfaces but also to generate an aesthetic shape according to the styling designers' feelings.
Counting Unstained, Confluent Cells by Modified Bright-Field Microscopy (2013)

We present a very simple procedure yielding high-contrast images of adherent, confluent cells such as human neuroblastoma (SH-EP) cells by ordinary bright-field microscopy. Cells are illuminated through a color filter and a pinhole aperture placed between the condenser and the cell culture surface. Refraction by each cell body generates a sharp, bright spot when the image is defocused. The technique allows robust, automatic cell counting from a single bright-field image in a wide range of focal positions using free, readily available image-analysis tools. Contrast may be enhanced by swelling cell bodies with a brief incubation in PBS. The procedure was benchmarked against manual and automated counting of fluorescently labeled cell nuclei. Counts from day-old and freshly seeded plates were compared in a range of densities, from sparse to densely overgrown. On average, bright-field images produced the same counts as fluorescence images, with less than 5% error. This method will allow routine cell counting using a plain bright-field microscope without cell-line modification or cell staining.
Principal Role of Glycoprotein VI in α2β1 and αIIbβ3 Activation During Collagen-Induced Thrombus Formation (2004)

GPVI- and autocrine-released ADP induce affinity changes of alpha2beta1 and alphaIIbbeta3 during thrombus formation on collagen under flow. These integrin changes are dispensable for adhesion but strengthen platelet-collagen interactions and thereby collagen-induced platelet activation. Integrin activation during thrombus formation on collagen was studied using fluorescent-labeled antibodies IAC-1 and PAC1 directed against activation-dependent epitopes of alpha2beta1 and alphaIIbbeta3 integrin, respectively. Glycoprotein VI blockade by 9O12 antibody or P2Y ADP receptors reduced integrin activation along with aggregate formation and fibrinogen binding but not alpha2beta1-dependent adhesion.
Development, testing and demonstration of a portable submersible miniature particle imaging velocimetry device (2007)

A portable underwater particle image velocimetry (PIV) device has been developed, tested and demonstrated. The underwater PIV uses a 532 nm battery-powered 90 mW continuous laser. The laser beam is pulsed via a camera-synchronized chopper wheel. Images were recorded using a 1 megapixel black and white 10-bit CCD battery-powered camera controlled via a PCMCIA frame grabber card connected to a laptop computer. The system was validated against a standard laboratory PIV for average velocities up to 15 cm s -1 downstream from a 1.6 cm circular cylinder. The average vorticities calculated between the two systems were similar with a maximum difference of 3.6%. The average velocities were also similar with the largest difference occurring at the slowest flow recorded (difference of 0.5 cm s -1 ), resulting in a 9.4% difference. The maximum eddy size was comparable between the two systems with an average error of 4%. The system was field tested in the Huron River, Michigan downstream from a 1.2 cm diameter submerged limb. Mean velocities and standard deviations were comparable to acoustic Doppler velocimeter data. This paper presents the first published subsurface PIV data from a fluvial environment, demonstrating potential applications for a number of ecological and geomorphological studies.
Comprehensive Census of Bacteria in Clean Rooms by Using DNA Microarray and Cloning Methods (2009)

A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servicing Facility (KSC-PHSF), and the Jet Propulsion Laboratory Spacecraft Assembly Facility (JPL-SAF) clean rooms were collected during the various assembly phases of the Phoenix and Mars Science Laboratory (MSL) spacecraft. Clone library-derived analyses detected a larger bacterial diversity prior to the arrival of spacecraft hardware in these clean room facilities. PhyloChip results were in agreement with this trend but also unveiled the presence of anywhere from 9- to 70-fold more bacterial taxa than cloning approaches. Among the facilities sampled, the JPL-SAF (MSL mission) housed a significantly less diverse bacterial population than either the LMA-MTF or KSC-PHSF (Phoenix mission). Bacterial taxa known to thrive in arid conditions were frequently detected in MSL-associated JPL-SAF samples, whereas proteobacterial lineages dominated Phoenix-associated KSC-PHSF samples. Comprehensive bacterial censuses, such as that reported here, will help space-faring nations preemptively identify contaminant biomatter that may compromise extraterrestrial life detection experiments. The robust nature and high sensitivity of DNA microarray technologies should prove beneficial to a wide range of scientific, electronic, homeland security, medical, and pharmaceutical applications and to any other ventures with a vested interest in monitoring and controlling contamination in exceptionally clean environments.
Supplier intelligence in MRO procurement (2008)

This paper explores the requirements for long term sustainable procurement practices. In the procurement of very expensive plant and equipment, MRO and the very long term continued availability of parts and components is very critical. Sustainable procurement practices are also critical in the continued non-disruption of critical parts and components in a manufacturing process. It proposes that procurement personnel and practices must have appropriate information and data of their prime suppliers of critical components, sub-assemblies and parts. Current research and literature search indicates there is very little publication and research investigating supplier intelligence (SI) in the context of the suppliers' suppliers' financial health, corporate governance, operating environment, capability, capacity and basic long term survival. This paper proposes the need for a conceptual model for gathering supplier's suppliers' intelligence for long term supplier relationship management; negotiation and contract management. The impetus for such information of a trading partner is becoming more critical since the collapse of large multi national corporations. Supplier intelligence is a fitness evaluation that enables the buyer to operationalise their long term procurement strategies with more certainty and lower risk. This paper also proposes how long term contractual terms and conditions may be designed taking into consideration of supplier's suppliers' profile to build superior supply chains and increase competitive advantage, hence a sourcing strategy.
Inhibitory effects of various ayurvedic and Panamanian medicinal plants on the infection of herpes simplex virus‐1 <i>in vitro</i> and <i>in vivo</i> (1995)

Abstract Extracts of 40 Ayurvedic medicines and 39 Panamanian medicinal plants were screened for their inhibitory activity on the plaque formation of herpes simplex virus type 1 (HSV‐1) in cultured cells. The extracts of 11 plant species showed potent inhibitory activity at a concentration of 100 μg/mL, while those of ten species showed moderate activities. Repeated oral administration of each extract of Rhus acuminata (galls), Saraca indica (bark), Strychnos potatrum (seeds) appreciably suppressed the development of typical skin lesions induced by infection of HSV‐1 in BALB/c mice. The extract of S. potatrum prolonged both development of skin lesion and mean survival time. This indicates that S. potatrum is a possible candidate for therapeutic application for HSV‐1 infection.
Formal implementation verification of the bus interface unit for the Alpha 21264 microprocessor (1997)

In this paper we present our method of formal verification of the transistor implementation of the Bus Interface Unit (BIU) of the Alpha 21264 microprocessor. We compare the logical description compiled from the Register Transfer Level (RTL) against that extracted from the custom-designed transistor-level schematics. BOVE, our BDD-based verification tool, does not require latch-to-latch correspondence, thus allowing the RTL to be more stable during the design process and giving the schematic designers freedom to implement race and timing optimizations. A unique "retiming" comparison algorithm efficiently compares partitions that include multiple pipeline stages, retiming optimizations and precharge logic. BOVE also verifies small finite-state machines that have different state encodings in the RTL and schematic.
Association between four SNPs in IL-4 and the risk of gastric cancer in a Chinese population. (2017)

Gastric cancer (GC) is the 5th most prevalent cancer. The etiology of GC is still poorly understood. We performed a case-control study in a Chinese population to investigate the association of rs2243248 (-1098 G/T), rs2227284 (-33 C/T), rs2243250 (-589 T/C) and rs2070874 (-107 T/C) polymorphisms and haplotypes with the development of gastric cancer in a Chinese population. A total of 362 patients with gastric cancer and 384 controls were recruited between December 2013 and December 2015. Genotyping of rs2243248 (-1098 G/T), rs2227284 (-33 C/T), rs2243250 (-589 T/C) and rs2070874 (-107 T/C) was performed in a 384-well plate format on the sequenom MassARRAY platform, and analyzed by MALDI-TOF MS. The TC and CC genotypes of rs2243250 (-589 T/C) were associated with an increased risk of gastric cancer when compared with the TT genotype, with adjusted ORs (95% CI) of 1.52 (1.07-2.15) and 2.13 (1.30-3.51), respectively. The TTTT haplotype revealed a reduced risk of gastric cancer (OR=0.65, 95% CI=0.45-0.94). No linkage disequilibrium was found among IL-4 rs2243248, rs2227284, rs2243250 and rs2070874. In summary, our findings support a significant association of IL-4 rs2243250 polymorphism with the risk of gastric cancer in the Chinese population, and IL-4 haplotype contributes to the development of this disease.
Potent interaction of flavopiridol with MRP1 (1999)

The multidrug resistance protein 1 (MRP1) is an ATP-dependent transport protein for organic anions, as well as neutral or positively charged anticancer agents. In this study we show that flavopiridol, a synthetic flavonoid currently studied in phase 1 trials for its antiproliferative characteristics, interacts with MRP1 in a potent way. Flavopiridol, as well as other (iso)flavonoids stimulate the ATPase activity of MRP1 in a dose-dependent way at low micromolar concentrations. A new specific monoclonal antibody against MRP1 (MIB6) inhibits the (iso)flavonoid-induced ATPase activity of plasma membrane vesicles prepared from the MRP1 overexpressing cell line GLC4/ADR. The accumulation of daunorubicin in GLC4/ADR cells is increased by flavopiridol and by other non-glycosylated (iso)flavonoids that interact with MRP1 ATPase activity. However, flavopiridol is the only tested compound that affects the daunorubicin accumulation when present at concentrations below 1 microM. Glycosylated (iso)flavonoids do not affect MRP1-mediated transport or ATPase activity. Finally, MRP1 overexpressing and transfected cells are resistant to flavopiridol, but not to other (iso)flavonoids tested. These findings may be of relevance for the development of anticancer therapies with flavopiridol.
Application of Xanthan Gum for Enhanced Oil Recovery (1977)

High molecular weight water soluble polymers find application in two different enhanced oil recovery processes. At present, the principal use is for an improved form of waterflooding in which polymers are used to increase the efficiency with which water can contact and displace reservoir oil. However, it is anticipated that polymer requirements for processes of this type will be overshadowed by the quantity needed to provide mobility control for future micellar-polymer projects. The latter processes have potential for producing oil that is unrecoverable by polymer augmented waterflooding. In both applications--polymer waterflooding and micellar-polymer flooding--the function of polymer is to reduce the mobility of injected water. Mobility is defined as the flow capacity of a rock-fluid system, or the volumetric flow rate per unit area achieved with a given pressure gradient (Figure 1). It is usually expressed as effective rock permeability divided by fluid viscosity, and the common petroleum reservoir engineering
Mycogenesis of cerium oxide nanoparticles using Aspergillus niger culture filtrate and their applications for antibacterial and larvicidal activities (2015)

Cerium oxide nanoparticles (CeO2 NPs) were synthesized using Aspergillus niger culture filtrate. The mycosynthesized CeO2 NPs were characterized by UV–Visible (UV–Vis), Fourier Transform Infrared (FT-IR), X-ray diffraction (XRD), Micro Raman, Thermogravimetric/Differential Thermal Analysis (TG/DTA), Photoluminescence, and Transmission Electron Microscopy (TEM) analyses. UV–Vis spectrum exhibited a corresponding absorption peak for CeO2 NPs at 296 nm, and the functional groups present in the fungal filtrate responsible for the synthesis of NPs were analyzed by FT-IR. The further characterization of the mycosynthesized CeO2 NPs revealed particles of the cubic structure and spherical shape, with the particle sizes ranging from 5 to 20 nm. The antibacterial activity of CeO2 NPs was examined in respect of two Gram-positive (G+) bacteria (Streptococcus pneumoniae, Bacillus subtilis) and two Gram-negative (G−) bacteria (Proteus vulgaris, Escherichia coli) by disk diffusion method. The test results for CeO2 NPs at a concentration of 10 mg/mL showed higher activities on the zone of inhibition of up to 10.67 ± 0.33 and 10.33 ± 0.33 mm against Streptococcus pneumonia and Bacillus subtilis, respectively, The CeO2 NPs caused 100 % mortality on first instar of Aedes aegypti at 0.250 mg/L concentration after 24-h exposure. The mycosynthesis of CeO2 NPs is a simple, cost-effective and eco-friendly approach and it will also potentially helpful to control pathogenic bacteria and dengue vector.
Spaceborne radar remote sensing: Applications and techniques (1988)

The operation and applications of spaceborne radars for terrestrial and planetary remote sensing are described in an introduction for advanced students and practicing scientists. Chapters are devoted to imaging radars, wave-surface interactions and geoscientific applications, real- and synthetic-aperture radars, end-to-end system design, SAR data processing, altimeters, and scatterometers. Extensive diagrams, drawings, graphs, photographs, and sample radar images are provided.
Application of lipase to concentrate the docosahexaenoic acid (DHA) fraction of fish oil (1991)

A commercial lipase preparation from Rhizopus niveus was used to concentrate the omega-3 fatty acid, docosahexaenoic acid (DHA) component in fish oil. The DHA content of cod-liver oil was 9.64% (w/w) of total fatty acids. Enzymatic digestion conditions were established which produced a DHA content in the monoglycerides fraction of 29.17% (w/w) of total fatty acid, triglyceride, and diglyceride components were 5.72, 9.95, and 15316%, respectively.
Molecular genetics of chromosome 21 and Down syndrome : proceedings of the Sixth Annual National Down Syndrome Society Symposium, held in New York, New York, December 7-8, 1989 (1990)

PART I - Molecular Structure of Human Chromosome 21 PART II - Genes on Chromosome 21 and the Phenotype of Down Syndrome PART III - Molecular Models of Down Syndrome.
Stereotaxic Apparatus for Operations on the Human Brain (1947)

Purpose: Head and neck cancer (HNC) is the sixth most common cancer worldwide with a 5-year survival rate of less than 50%. The PI3K/AKT/mTOR signaling pathway is frequently implicated in HNC. Recently, IQ motif–containing GTPase-activating protein 1 (IQGAP1) was discovered to scaffold the PI3K/AKT signaling pathway. IQGAP1 gene expression is increased in HNC, raising the hypothesis that IQGAP1 contributes to HNC. Experimental Design: We performed a combination of in vitro studies using human cancer cell lines treated with a cell-permeable peptide that interferes with IQGAP1′s ability to bind to PI3K, and in vivo studies utilizing mice genetically knocked out for the Iqgap1 (Iqgap1−/−). In vivo EGF stimulation assays were used to evaluate PI3K signaling. To study the role of IQGAP1 in HNC, we used a well-validated mouse model that drives HNC via a synthetic oral carcinogen, 4-nitroquinoline 1-oxide (4NQO). Results: IQGAP1 is necessary for efficient PI3K signaling in vitro and in vivo. Disruption of IQGAP1-scaffolded PI3K/AKT signaling reduced HNC cell survival. Iqgap1−/− mice had significantly lower cancer incidences, lesser disease severity, and fewer cancer foci. IQGAP1 protein levels were increased in HNC arising in Iqgap1+/+ mice. The level of PI3K signaling in 4NQO-induced HNC arising in Iqgap1−/− mice was significantly reduced, consistent with the hypothesis that IQGAP1 contributes to HNC at least partly through PI3K signaling. High IQGAP1 expression correlated with reduced survival, and high pS6 levels correlated with high IQGAP1 levels in patients with HNC. Conclusions: These data demonstrate that IQGAP1 contributes to head and neck carcinogenesis.