gebhart/patrepeval huggingface dataset:
Calcium-Regulating Hormones in Essential Hypertension (1986)

Circulating levels of the calcium-regulating hormones, calcitonin, calcitriol, and parathyroid hormone, were analyzed in relation to plasma renin activity in 10 persons with normal blood pressure and in 51 persons with essential hypertension. Calcitriol (p less than 0.008) and parathyroid hormone (p less than 0.01) levels were elevated in hypertensives with low renin activity, whereas calcitonin levels were higher in patients with high renin activity (p less than 0.008), compared with normotensive controls and other hypertensive patients. Continuous relationships were observed between calcitriol levels and plasma renin activity in all patients (r = -0.65, p less than 0.001) and between parathyroid hormone levels and urinary sodium excretion in hypertensive patients with low renin activity (r = -0.63, p less than 0.01). Together, these results support a linkage between calcium metabolism and renin-sodium factors in essential hypertension. Calcium-regulating hormones and the renin-aldosterone system may coordinately mediate the blood pressure effects of differing dietary calcium and sodium intakes at the cellular level by altering cellular handling of monovalent and divalent ions.
Effect of Self-Dispersion Nanosized AlOOH on Microstructure and Wear Resistance of Alumina Ceramic Balls (2008)

The self-dispersed AlOOH nanopowders were prepared by the sol-hydrothermal crystallization and charging composite dispersion method. The nanosized AlOOH were dispersed evenly into the mixed slurry of alumina and fluxing agent by the new batching technology of sol homogeneous dispersion and ball-milling-free for preparation of the ceramic body containing 98.1% alumina. The ceramic ball blank formed by cold isostatic pressing was sintered into ceramic ball at ordinary pressure and the temperature lower than 1500°C for 3 h. Using this method, uniform and fine grains of ceramic ball with better wear resistance were obtained by adding 3.5 mass% nanosized AlOOH to ceramic slurry. The wear rate of the self-made ceramic ball and the compared ceramic ball (which is the most wear resistance ceramic ball at present) under the same testing conditions were 0.004 and 0.056%/h, respectively.
An all-transputer visual autobahn-autopilot/copilot (2002)

An integrated system for visual road vehicle guidance has been created on transputer-hardware and partly validated in normal Autobahn traffic environments. The computer vision and dynamic scene understanding components are discussed. The system is capable of recognizing horizontal and vertical curvature of the lane, lane width, number of lanes, the state of the vehicle relative to the lane, and the relative state of two other objects by recursive monocular estimation following the 4-D approach as a unifying scheme for 3-D dynamic scene understanding through motion stereo. The architecture realized on transputer hardware is described. Experimental results with a 5-ton van are discussed.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The eag Potassium Channel Binds and Locally Activates Calcium/Calmodulin-dependent Protein Kinase II (2004)

Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) has been implicated in the regulation of neuronal excitability in many systems. Recent studies suggest that local regulation of membrane potential can have important computational consequences for neuronal function. In Drosophila, CaMKII regulates the eag potassium channel, but if and how this regulation was spatially restricted was unknown. Using coimmunoprecipitation from head extracts and in vitro binding assays, we show that CaMKII and Eag form a stable complex and that association with Eag activates CaMKII independently of CaM and autophosphorylation. Ca(2+)/CaM is necessary to initiate binding of CaMKII to Eag but not to sustain association because binding persists when CaM is removed. The Eag CaMKII-binding domain has homology to the CaMKII autoregulatory region, and the constitutively active CaMKII mutant, T287D, binds Eag Ca(2+)-independently in vitro and in vivo. These results favor a model in which the CaMKII-binding domain of Eag displaces the CaMKII autoinhibitory region. Displacement results in autophosphorylation-independent activation of CaMKII which persists even when Ca(2+) levels have gone down. Activity-dependent binding to this potassium channel substrate allows CaMKII to remain locally active even when Ca(2+) levels have dropped, providing a novel mechanism by which CaMKII can regulate excitability locally over long time scales.
The heat capacity of nickel carbonyl and the thermodynamics of its formation from nickel and carbon monoxide (1955)

J. E. Spice, L. A. K. Staveley and G. A. Harrow, J. Chem. Soc., 1955, 100 DOI: 10.1039/JR9550000100
Reverse engineering of protein secretion by uncoupling of cell cycle phases from growth (2011)

The demand for recombinant proteins both for biopharmaceutical and technical applications is rapidly growing, and therefore the need to establish highly productive expression systems is steadily increasing. Yeasts, such as Pichia pastoris, are among the widely used production platforms with a strong emphasis on secreted proteins. Protein secretion is a limiting factor of productivity. There is strong evidence that secretion is coupled to specific growth rate (µ) in yeast, being higher at higher µ. For maximum productivity and product titer, high specific secretion rates at low µ would be desired. At high secretion rates cultures contain a large fraction of cells in the G2 and M phases of cell cycle. Consequently, the cell design target of a high fraction of cells in G2 + M phase was achieved by constitutive overexpression of the cyclin gene CLB2. Together with predictive process modeling this reverse engineered production strain improved the space time yield (STY) of an antibody Fab fragment by 18% and the product titer by 53%. This concept was verified with another secreted protein, human trypsinogen.
A model driven component-based development framework for agents (2005)

Developing agent-oriented systems is still a difficult task. However, a component-based approach can help by supporting both modular modification of existing systems and construction of new systems from existing parts. In this paper we develop a foundation (conceptual model) for a component-based agent development framework by extending the concepts of the SMART framework. We apply our definitions to an existing agent application in order to both refine the definitions, and to evaluate the extent to which the model is able to support modification of existing systems. A prototype toolkit called Component Agent Framework for non-Experts (CAFnE) that implements the component framework is also explained briefly.
Vertically Aligned <sup>13</sup>C Single-Walled Carbon Nanotubes Synthesized by No-Flow Alcohol Chemical Vapor Deposition and their Root Growth Mechanism (2008)

We present the successful synthesis of aligned 13C labeled single-walled carbon nanotube (SWNT) arrays from alcohol by a modified no-flow chemical vapor deposition (CVD) method that makes efficient growth possible using a small amount of carbon source. The synthesis of high-quality SWNTs by this alternative method was confirmed by resonance Raman spectroscopy, which also showed that the quality of the grown SWNTs is uniform in growth direction. The synthesis of 13C labeled SWNTs provides solid evidence for the root growth mechanism in alcohol catalytic CVD, which agrees well with the transmission electron microscopy (TEM) observations.
The 'universal' dielectric response. III (1990)

The screened hopping mechanism, which assumes that in every dielectric system, dipolar or hopping-charge-dominated, there must be a finite amount of local adjustment of dipolar orientations or of charge dispositions in response to any particular dipole or charge movement, is discussed. The relaxation of polarization in a dipolar system with a frequency-domain (FD) behavior is considered, focusing on the application of the energy criterion to such systems at low frequencies of the applied alternating field. The spectral shape of the dielectric loss characteristic of dipolar materials in the FD, and the corresponding time-domain (TD) response are reexamined in the light of the foregoing discussion. An electrochemical model of low-frequency dispersion is presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Advancements in Paraffin Testing Methodology (2001)

Abstract Predictive methods for wax deposition within pipelines make extensive use of diffusion models to account for the amount of wax being deposited. Laboratory testing methods commonly rely on deposition results from so-called coldfinger devices to assess deposition tendencies. While these experiments are primarily done for testing chemical inhibitor performance, they serve here as the basis for a cross-reference to the often-used diffusion models. An empirical heat transfer model is developed to adequately address the heat transfer within this device. Oil analysis data in combination with thermodynamic prediction routines are used to prepare the necessary n-paraffin solubility charts. While the heat transfer and solubility data are used for a diffusion-type wax deposition, an empirical deposition model is developed to describe the deposition process. It is illustrated how practical information such as deposition tendencies can be gained from these cold finger devices. An assessment of chemical inhibitor performance is made.
METTL3 inhibition reduces N<sup>6</sup>‐methyladenosine levels and prevents allogeneic CD4<sup>+</sup> T‐cell responses (2022)

Alloreactive CD4<sup>+</sup> T cells play a central role in allograft rejection. However, the post-transcriptional regulation of the effector program in alloreactive CD4<sup>+</sup> T cells remains unclear. N<sup>6</sup> -methyladenosine (m<sup>6</sup> A) RNA modification is involved in various physiological and pathological processes. Herein, we investigated whether m<sup>6</sup> A methylation plays a role in the allogeneic T-cell effector program. m<sup>6</sup> A levels of CD4<sup>+</sup> T cells from spleens, draining lymph nodes and skin allografts were determined in a skin transplantation model. The effects of a METTL3 inhibitor (STM2457) on CD4<sup>+</sup> T-cell characteristics including proliferation, cell cycle, cell apoptosis and effector differentiation were determined after stimulation of polyclonal and alloantigen-specific (TEa; CD4<sup>+</sup> T cells specific for I-Eα<sub>52-68</sub> ) CD4<sup>+</sup> T cells with α-CD3/α-CD28 monoclonal antibodies and cognate CB6F1 alloantigen, respectively. We found that graft-infiltrating CD4<sup>+</sup> T cells expressed high m<sup>6</sup> A levels. Administration of STM2457 reduced m<sup>6</sup> A levels, inhibited T-cell proliferation and suppressed effector differentiation of polyclonal CD4<sup>+</sup> T cells. Alloreactive TEa cells challenged with 40 μm STM2457 exhibited deficits in T-cell proliferation and T helper type 1 cell differentiation, a cell cycle arrest in the G0 phase and elevated cell apoptosis. Moreover, these impaired T-cell responses were associated with the diminished expression levels of transcription factors Ki-67, c-Myc and T-bet. Therefore, METTL3 inhibition reduces the expression of several key transcriptional factors for the T-cell effector program and suppresses alloreactive CD4<sup>+</sup> T-cell effector function and differentiation. Targeting m<sup>6</sup> A-related enzymes and molecular machinery in CD4<sup>+</sup> T cells represents an attractive therapeutic approach to prevent allograft rejection.
A review of substrate coupling issues and modeling strategies (2003)

This tutorial paper reviews the technologies behind substrate coupling and modeling. The key focuses throughout are the importance of suitable substrate models and a sufficient understanding of the substrate coupling in the circuit being designed. Discussions include Radio Frequency (RF) and Mixed-Signal (MS) designs, but also touch upon System-on-Chip (SoC). An example RF circuit is simulated with a substrate model and the effect of substrate process variations on the circuit's performance is discussed.
Use of a sensitive bioimmunoabsorbent assay to isolate and characterize monoclonal antibodies to biologically active human erythropoietin (1988)

Abstract At present, one of the most sensitive assays for human erythropoietin (Ep) is a bioassay that measures the Ep-dependent proliferation of spleen cells from phenylhydrazine-treated mice after 24 hours in culture. We describe how this assay can be used as the basis of a very sensitive method for detecting mouse antibodies to biologically active human Ep. In this procedure, microtiter wells are first coated with goat anti-mouse Ig antibody, then treated with mouse antibodies (serum or hybridoma culture supernatants), and finally incubated with a fixed amount of pure human Ep. Specific binding of anti-Ep antibodies is detected by adding spleen cells from phenylhydrazine-treated mice to the wells and measuring the ability of the cells to incorporate 3H- thymidine 24 hours later. This bioimmunosorbent assay (BISA) revealed the presence of anti-EP antibodies in sera from mice immunized with either pure human urinary Ep or a synthetic dodecapeptide corresponding to the aminoterminal region of Ep and in the culture supernatants from three of eight stable anti-Ep antibody-producing hybridoma cell lines that we have isolated. The three monoclonal antibodies showed similar reactivities in the BISA, but showed different affinities for Ep, with Kd values of approximately 0.7, 8, and 240 nmol/L, respectively. Further studies showed that all antibodies were capable of neutralizing Ep bioactivity and of binding 125I-labeled Ep in a radioimmunosorbent assay (RIA) but were virtually unreactive to Ep adsorbed to the bottom of enzyme-linked immunosorbent assay (ELISA) wells. Our results suggest that the BISA strategy may be an important complement to conventional RIA and ELISA techniques for identification of monoclonal antibodies specific for biologically active growth factors.
Automatic Calibration of Frequency Compensation System in Computer-Controlled Patch-Clamp Amplifier (2007)

Computer-controlled patch-clamp amplifier is a digitally controlled analog device used to record the cellular ion channel currents in electrophysiology research. The inherent bandwidth and performance of the headstage is limited by the stray capacitance and distributed capacitance across the feedback resistors. In order to effectively improve the performance of the headstage, the paper advanced a simplified automatic calibration method of frequency compensation system in resistor-feedback patch-clamp amplifier. The dynamic model of headstage was approximate as a two poles and one zero system in the transfer function by experience and test results, so the dynamic characteristics of the headstage were obtained employing least squares parameter estimation algorithm. Further more, the compensation parameter of high frequency booster can be estimated by the time constant of main pole of headstage. And automatic adjustment of the parameters in transient response correction stage was performed as a least squares fitting problem. The software routine running on the host computer conducted all operations of frequency compensation. Experimental results demonstrate that the simplified automatic calibration method can substantially extend the bandwidth and minimize step response error of headstage.
On the minimum distance of array codes as LDPC codes (2003)

For a prime q and an integer j/spl les/q, the code C(q,j) is a class of low-density parity-check (LDPC) codes from array codes which has a nice algebraic structure. In this correspondence, we investigate the minimum distance d(q,j) of the code in an algebraic way. We first prove that the code is invariant under a doubly transitive group of "affine" permutations. Then, we show that d(5,4)=8, d(7,4)=8, and d(q,4)/spl ges/10 for any prime q>7. In addition, we also analyze the codewords of weight 6 in the case of j=3 and the codewords of weight 8 in C(5,4) and C(7,4).
Fetal fibronectin. (2002)

Evaluation of sensitivity and specificity studies document that the fetal fibronectin test predicts preterm delivery. For symptomatic women, a sensitivity of 89% and specificity of 86% was found.
Chiral bis(amino acid)- and bis(amino alcohol)-oxalamidegelators. Gelation properties, self-assembly motifs and chirality effects (2009)

Bis(amino acid)- and bis(amino alcohol)oxalamide gelators represent the class of versatile gelators whose gelation ability is a consequence of strong and directional intermolecular hydrogen bonding provided by oxalamide units and lack of molecular symmetry due to the presence of two chiral centres. Bis(amino acid)oxalamides exhibit ambidextrous gelation properties, being capable to form gels with apolar and also highly polar solvent systems and tend to organise into bilayers or inverse bilayers in hydrogel or organic solvent gel assemblies, respectively. (1)H NMR and FTIR studies of gels revealed the importance of the equilibrium between the assembled network and smaller dissolved gelator assemblies. The organisation in gel assemblies deduced from spectroscopic structural studies are in certain cases closely related to organisations found in the crystal structures of selected gelators, confirming similar organisations in gel assemblies and in the solid state. The pure enantiomer/racemate gelation controversy is addressed and the evidence provided that rac-16 forms a stable toluene gel due to resolution into enantiomeric bilayers, which then interact giving gel fibres and a network of different morphology compared to its (S,S)-enantiomer gel. The TEM investigation of both gels confirmed distinctly different gel morphologies, which allowed the relationship between the stereochemical form of the gelator, the fibre and the network morphology and the network solvent immobilisation capacity to be proposed. Mixing of the constitutionally different bis(amino acid) and bis(amino alcohol)oxalamide gelators resulted in some cases in highly improved gelation efficiency denoted as synergic gelation effect (SGE), being highly dependent also on the stereochemistry of the component gelators. Examples of photo-induced gelation based on closely related bis(amino acid)-maleic acid amide and -fumaramide and stilbene derived oxalamides where gels form by irradiation of the solution of a non-gelling isomer and its photo-isomerisation into gelling isomer are provided, as well as examples of luminescent gels, gel-based fluoride sensors, LC-gels and nanoparticle-hydrogel composites.
Detection of endogenous human cytomegalovirus in CD34+ bone marrow progenitors (1996)

The cellular sites and mechanisms of human cytomegalovirus (HCMV) latency are still poorly defined. Although evidence suggests that peripheral blood monocytes are one site of latency in the healthy carrier, it is unlikely that monocytes represent a site of primary HCMV infection. Consequently, we have analysed CD34+ bone marrow progenitors, precursors of monocytes, to determine whether they are a site of HCMV carriage in normal virus carriers. For the first time, we demonstrate the presence of endogenous HCMV within bone marrow progenitors in the absence of HCMV lytic gene expression. These findings are consistent with previous evidence showing that the permissiveness of myeloid cells for HCMV is critically dependent on the differentiation state of the cell.
Construction, Properties, and Analysis of Group-Orthogonal Supersaturated Designs (2019)

In this article, we propose a new method for constructing supersaturated designs that is based on the Kronecker product of two carefully chosen matrices. The construction method leads to a partitioning of the factors of the design such that the factors within a group are correlated to the others within the same group, but are orthogonal to any factor in any other group. We refer to the resulting designs as group-orthogonal supersaturated designs. We leverage this group structure to obtain an unbiased estimate of the error variance, and to develop an effective, design-based model selection procedure. Simulation results show that the use of these designs, in conjunction with our model selection procedure enables the identification of larger numbers of active main effects than have previously been reported for supersaturated designs. The designs can also be used in group screening; however, unlike previous group-screening procedures, with our designs, main effects in a group are not confounded. Supplementary materials for this article are available online.
Promoters for the (alkyne) hexacarbonyldicobalt-based cyclopentenone synthesis (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPromoters for the (alkyne) hexacarbonyldicobalt-based cyclopentenone synthesisYoung Keun Chung, Bun Yeoul Lee, Nakcheol Jeong, Milan Hudecek, and Peter L. PausonCite this: Organometallics 1993, 12, 1, 220–223Publication Date (Print):January 1, 1993Publication History Published online1 May 2002Published inissue 1 January 1993https://pubs.acs.org/doi/10.1021/om00025a036https://doi.org/10.1021/om00025a036research-articleACS PublicationsRequest reuse permissionsArticle Views875Altmetric-Citations149LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Multilevel channel assignment (MCA) for wireless personal communications (2002)

A channel allocation technique to guarantee different carrier-to-interference (C/I) ratio constraints in wireless networks supporting multiple services is proposed and analyzed through a system supporting two services, voice and data. Since these services require different bit error rate (BER) performance, different C/I values should be used to meet these requirements. To achieve this goal a cell is divided into concentric zones with the objective of trading off C/I performance of users according to their service class and geographical location. The underlying principle is to offer the minimum required C/I performance for voice users in the inner zone having more than adequate transmission quality in order to improve C/I performance for data users which require very low BER. A Markov chain model of the proposed scheme is identified and results obtained through a comprehensive computer simulation for voice call blocking probability and data access delay are provided.