gebhart/patrepeval huggingface dataset:
Physical and Functional Interaction of Acyl-CoA-binding Protein with Hepatocyte Nuclear Factor-4α (2003)

Although acyl-CoA-binding protein (ACBP) has been detected in the nucleus, the physiological significance of this observation is unknown. As shown herein for the first time, ACBP in the nucleus physically and functionally interacted with hepatocyte nuclear factor-4 alpha (HNF-4 alpha), a nuclear binding protein that regulates transcription of genes involved in both lipid and glucose metabolism. Five lines of evidence showed that ACBP bound HNF-4 alpha in vitro and in the nucleus of intact cells. (i) ACBP interaction with HNF-4 alpha elicited significant changes in secondary structure. (ii) ACBP and HNF-4 alpha were coimmunoprecipitated by antibodies to each protein. (iii) Double immunolabeling and laser scanning confocal microscopy (LSCM) of rat hepatoma cells and transfected COS-7 cells significantly colocalized ACBP and HNF-4 alpha within the nucleus and in the perinuclear region close to the nuclear membrane. (iv) LSCM fluorescence resonance energy transfer determined an intermolecular distance of 53 A between ACBP and HNF-4 alpha in rat hepatoma cell nuclei. (v) Immunogold electron microscopy detected ACBP within 43 A of HNF-4 alpha. These interactions were specific since ACBP did not interact with Sp1 or glucocorticoid receptor in these assays. The functional significance of ACBP interaction with HNF-4 alpha was evidenced by mammalian two-hybrid and transactivation assays. ACBP overexpression in COS-7 or rat hepatoma cells enhanced transactivation of an HNF-4 alpha-dependent luciferase reporter plasmid by 3.2- and 1.6-fold, respectively. In contrast, cotransfection with antisense ACBP expression vector inhibited transactivation. LSCM of the individual triple fluorescent-labeled (HNF-4 alpha, ACBP, and luciferase) rat hepatoma cells showed a high correlation (r2, 0.936) between the level of luciferase and the level of ACBP expression. In summary, ACBP physically interacted with HNF-4 alpha in vitro and in intact cells, although ACBP expression level directly correlated with HNF-4 alpha-mediated transactivation in individual cells.
Pharmacokinetic and Biodistribution Studies of a Bone-Targeting Drug Delivery System Based on<i>N</i>-(2-Hydroxypropyl)methacrylamide Copolymers (2006)

Osteotropicity of novel bone-targeted HPMA copolymer conjugates has been demonstrated previously with bone histomorphometric analysis. The pharmacokinetics and biodistribution of this delivery system were investigated in the current study with healthy young BALB/c mice. The 125I-labeled bone-targeted and control (nontargeted) HPMA copolymers were administered intravenously to mice, and their distribution to different organs and tissues was followed using gamma counter and single photon emission computed tomography (SPECT). Both the invasive and noninvasive data further confirmed that the incorporation of D-aspartic acid octapeptide (D-Asp8) as bone-targeting moiety could favorably deposit the HPMA copolymers to the entire skeleton, especially to the high bone turnover sites. To evaluate the influence of molecular weight, three fractions (Mw of 24, 46, and 96 kDa) of HPMA copolymer-D-Asp8 conjugate were prepared and evaluated. Higher molecular weight of the conjugate enhanced the deposition to bone due to the prolonged half-life in circulation, but it weakened the bone selectivity. A higher content of bone-targeting moiety (D-Asp8) in the conjugate is desirable to achieve superior hard tissue selectivity. Further validation of the bone-targeting efficacy of the conjugates in animal models of osteoporosis and other skeletal diseases is needed in the future.
A fluoroscopic-based navigation system for ACL reconstruction assisted by robot (2009)

Entry position of the graft is very important in anterior cruciate ligament (ACL) reconstruction. However the determination of entry position is very difficult to the surgeon. In this paper, a navigation and evaluation system assisted by the 6-DOFS robot is implemented for the simulation evaluation and planning insertion points based on quadrant method for the femur and Stäublis method for the tibia on the lateral X-ray image of knee joint. Meanwhile, the implementation of the key technologies such as image correction, image registration, C-arm calibration, video tracking, bone surface reconstruction, image fusion, 6-DOFS robot, and virtual simulation are introduced. Finally, Experiments about the tunnel planning method and real time tracking of surgical apparatus are implemented on 8 bone of plastic models (Sawbone, Swiss) and 10 bones of the goat. In the experiment, the tibia rotates around the femur under the surgeon's implementation to evaluate the planning result with the virtual simulation and evaluation module. The positioning error is 1.59mm from analysis on 30 space targets. The virtual reconstruction ACL is satisfied with two important criteria of the best isometry and collision detection between graft and intercondylar surface of femur. The results are well accepted in operations. In order to satisfying with the request of exact operation in ACL reconstruction, we have developed 6-DOFS passive robot to assist the surgeons entry positioning and drilling of implant tunnels, implementing exact operation in knee joint.
CO<sub>2</sub> Capture by As‐Prepared SBA‐15 with an Occluded Organic Template (2006)

Abstract A novel CO 2 capture phenomenon is observed by modifying as‐prepared mesoporous silica SBA‐15 (SBA(P)) with tetraethylenepentamine (TEPA), not only conserving the energy and time required for removing the template, but also opening the way to utilizing the micelle for dispersing guest species. The TEPA species dispersed within the channels of SBA(P) are highly accessible to CO 2 molecules; moreover, the hydroxyl group of the poly(ethylene oxide)–poly(propylene oxide)–poly(ethylene oxide) (Pluronic P123) template is able to modify the interactions between CO 2 and the amine to enhance the adsorptive capacity of this system. The remarkably high adsorption capacity (173 mg g –1 ) of this mesoporous silica–amine composite suggests potential CO 2 trapping applications, especially at low CO 2 concentrations during prolonged cyclic operations.
Direct Deposition of Amorphous Cobalt–Vanadium Mixed Oxide Films for Electrocatalytic Water Oxidation (2019)

Efficiency of water oxidation catalysts in terms of overpotential, current density, and voltage stability over time with facile methods of their fabrication remains a key challenge in developing competent mechanisms of storing energy in the form of green hydrogen fuels. In this work, a rapid one-step aerosol-assisted chemical vapor deposition (AACVD) method is employed to synthesize amorphous and highly active cobalt-vanadium mixed oxide catalysts (CoVO<sub>x</sub>) directly over fluorine-doped tin oxide (FTO) substrates. Morphological and structural characterizations made by field emission scanning electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and X-ray photoelectron spectroscopy techniques revealed the formation of pure-phase amorphous films with a gradual variation of topography as a function of deposition time. Of these films, the most active film (CoVO<sub>x</sub>-20) was obtained in 20 min deposition, showing a spongy networking of interwoven nanofibers with a homogeneous distribution of 3-4 nm pores, achieving an overpotential of 308 mV at 10 mA/cm<sup>2</sup> current density. A much higher current density of 175 mA/cm<sup>2</sup> could be achieved just at 380 mV of overpotential with Tafel slope as low as 62 mV/dec for this whole range while exhibiting long-term stability. Mass activity, electrochemical impedance spectroscopy data, and the estimation of electrochemically active surface area all endorsed this high catalytic performance of CoVO<sub>x</sub>-20, which is unprecedented for a low-cost, upscalable, and relatively less conductive substrate such as FTO used here. Our findings, thus, not only highlight the benefits of using AACVD in preparing two-dimensional amorphous catalysts but also prove the high efficiency of CoVO<sub>x</sub> materials thus obtained, as outlined in a plausible reaction mechanism.
Newly formed excitatory pathways provide a substrate for hyperexcitability in experimental temporal lobe epilepsy (1999)

Temporal lobe epilepsy (TLE) in humans and animals is associated with axonal sprouting of glutamatergic neurons and neosynaptogenesis in the hippocampal formation. We examined whether this plasticity of excitatory pathways contributes to an increased level of glutamatergic excitation in the CA1 region of rats experiencing chronic spontaneous limbic seizures following kainic acid or pilocarpine treatment. In chronic cases, we report an extensive axonal sprouting of CA1 pyramidal neurons, with many axonal branches entering the pyramidal cell layer and stratum radiatum, regions that are not innervated by axonal collaterals of CA1 pyramidal neurons in control animals. Concurrently with this anatomical reorganization, a large increase of the spontaneous glutamatergic drive is observed in the dendrites and somata of CA1 pyramidal cells. Furthermore, electrical activation of the reorganized CA1 associational pathway evokes epileptiform bursts in CA1 pyramidal cells. These findings suggest that reactive plasticity could contribute to the hyperexcitability of CA1 pyramidal neurons and to the propagation of seizures in these two models of TLE.
A method for mapping magnetic fields generated by current coils (1985)

Describes a device for measurement, control and mapping of magnetic fields. The magnetic sources, mainly coils, are powered by an alternating current; a pick-up coil detects the induced EMF, which is amplified by a phase-sensitive detector. Operating at low frequencies (below 100 Hz) substantially reduces the eddy current effects when conducting materials are near the coils. An application to control the linearity of a magnetic gradient is indicated. The simplicity of this device makes it very efficient in the design of gradient coil systems for nuclear magnetic resonance imaging.
Age-related comparisons by sex in the domains of aerobic physical activity for adults in Scotland (2015)

The domains in which adults in Scotland undertake MVPA vary by age group. Policies designed to increase PA should take this into account. Our findings challenge current thinking on age-related changes in activity, with the exclusion of occupational activity mitigating any age-related decline in MVPA.
Rhodium(II)-Catalyzed Stereocontrolled Synthesis of Dihydrofuran-3-imines from 1-Tosyl-1,2,3-triazoles (2014)

Rhodium(II) acetate catalyzes the denitrogenative transformation of 5-substituted and 4,5-disubstituted 1-sulfonyl-1,2,3-triazoles with pendent allyl and propargyl ether motifs to oxonium ylides that undergo [2,3]-sigmatropic rearrangement to give substituted dihydrofuran-3-imines in high yield and diastereoselectivity.
청국장 점질물의 생리기능 특성 (2013)

우리나라 대두 발효식품의 하나인 청국장의 우수성을 입증하고자 청국장 점질물을 이용하여 glucose 및 bile acid 투과 억제 효과, fibrinolytic activity, 그리고 장내세균 이용성 확인하였다. 청국장으로부터 점질물을 분리한 결과 2.6%의 수율을 보였다. Glucose 흡수지연효과는 2시간에 19.7%의 효과를 보였고, Bile acid 흡수지연효과는 30.0%의 효과를 보였다. 청국장 점질물의 fibrinolytic activity는 5g/100mL의 농도에서 140%로 높은 혈전 용해 활성을 나타내었다. 장내균총 중 유익균인 Bifidobacterium spp.에 대한 점질물의 증식저해 및 증진 효과를 살펴본 결과, Bifidobacterium adolescentis, Bifidobacterium bifidum, Bifidobacterium longum 모두 증식이 촉진되었다. 이상의 결과로 전통 청국장의 생리기능 특성에 대한 우수성이 입증되었다.
Changes in cytokine and biomarker blood levels in patients with colorectal cancer during dendritic cell-based vaccination (2009)

INTRODUCTION. Immunotherapy based on dendritic cell vaccination has exciting perspectives for treatment of cancer. In order to clarify immunological mechanisms during vaccination it is essential with intensive monitoring of the responses. This may lead to optimization of treatment and prediction of responding patients. The aim of this study was to evaluate cytokine and biomarker responses in patients with colorectal cancer treated with a cancer vaccine based on dendritic cells pulsed with an allogeneic melanoma cell lysate. MATERIAL AND METHODS. Plasma and serum samples were collected prior to vaccination and continuously during treatment. GM-CSF, IL-2, IL-6, TNF-alpha, IFN-gamma, IL-4, IL-8, IL-1b, IL-5, IL-10, IL-12, MIP-1b, IP-10 and Eotaxin were analyzed in a multiplex assay with a Luminex 100 instrument. CEA and TIMP-1 were analysed on ELISA platforms. RESULTS. Patients achieving stable disease showed increasing levels of plasma GM-CSF, TNF-alpha, IFN-gamma, IL-2, and IL-5. Patients with progressive disease showed significant increase in CEA and TIMP-1 levels, while patients with stable disease showed relatively unaltered levels. CONCLUSION. The increased levels of key pro-inflammatory cytokines in serum of patients who achieved stable disease following vaccination suggest the occurrence of vaccine-induced Th1 responses. Since Th1 responses seem to be essential in cancer immunotherapy this may indicate a therapeutic potential of the vaccine.
Towards a verified component platform (2013)

This paper describes ongoing work on a new technique for reducing the cost of assurance of large software systems by building on a verified component platform. From a component architecture description, we automatically derive a formal model of the system and a semantics for the runtime behaviour of generated inter-component communication code. We can prove wellformedness properties of the architecture automatically and provide a framework in which users can reason about their component code and its behaviour. By leveraging the isolation properties and communication guarantees of a formally verified platform, correctness arguments for critical components will be able to be derived independently and composed together to reason about system-level correctness.
Syntheses of α-Pyrones Using Gold-Catalyzed Coupling Reactions (2011)

Sequential alkyne activation of terminal alkynes and propiolic acids by gold(I) catalysts yields compounds having α-pyrone skeletons. Novel cascade reactions involving propiolic acids are reported that give rise to α-pyrones with different substitution patterns.
Purification and composition of protein antifreezes with high cysteine contents from larvae of the beetle, <i>Tenebrio molitor</i> (1982)

Abstract Two antifreeze proteins with thermal hysteresis activity (they depress the freezing point of aqueous solutions by a noncolligative mechanism well below the melting point) were purified from cold‐acclimated larvae of the beetle, Tenebrio molitor . Both proteins have unusual amino acid compositions consisting of high levels of cysteine (15.4 and 28.0 mol%).
Integration Of Structured And Unstructured Text Data In A Clinical Information System (2006)

This paper describes an integration of structured and unstructured text data in a Clinical Information System (CIS). The input and output data of this Clinical Information System depend heavily on the use of a medical database and an intensive Graphic User Interface (GUI). The data source consists of multiple GUI's fields of various forms which compose structured data and free-text medical reports of patients. These free text medical reports are mostly made of the complicated part of unstructured text data. Usually, the free text medical reports include reports on patient intake, examination, and discharge, which could be an input from a keyboard, a handwriting device, a voice microphone, transcription service or others. Some of the medical language extraction and encoding systems, such as MedLEE, can be used to translate the medical free text into a standard and structured format. These systems will be reviewed and adopted to perform the knowledge extraction from medical free texts, if applicable. To achieve the goal of data sharing for the future application in data interoperability between heterogeneous database systems, a clinical data repository can be stored which is formed by applying a standard-compliant eXtensible Markup Language (XML), with GUI form, for integrating these data. Currently there are a number of healthcare information standards, such as HL7 (Health Level 7, 2006) and Medical Markup Language (Medical Markup Language, 2006) to promote data exchange. But it requires that both the sender and receiver of the data use the same standard in order to achieve the data exchange goal. In this paper, the generic data XML model is therefore developed and the feasibility for transforming the model into any of the standards is also demonstrated.
Kinetic Aspects of Cyanmethemoglobin Formation from Carboxyhemoglobin (1967)

Abstract The conversion of carboxy hemoglobin (HbCO) to cyanmethemoglobin (MHbCN) by Drabkin's reagent has been shown to be considerably slower than is the same reaction with oxyhemoglobin(Hb02). Conversion of Hb02 to MHbCN is complete in 10 min. whereas total conversion of HbCO requires 90-120 min. This difference in reaction rate must be considered in the determination of total hemoglobin as MHbCN when the blood contains appreciable HbCO.
A cerebellar-like terminal and postural tremor induced in normal man by transcranial magnetic stimulation (1999)

Trains of repetitive transcranial magnetic stimulation (TMS) at 10-30 Hz and intensities of 90-120% motor threshold were delivered through a figure of eight coil over the motor cortex while normal subjects made either rapid, self-terminated (ballistic) wrist movements or maintained the position of their wrist at a fixed angle. Movement kinematics and EMG activity in antagonistic forearm muscles were analysed. In the ballistic task, repetitive TMS had little effect on the velocity or acceleration of the initial segment of the movement, although it induced large terminal oscillations (tremor) around the target position at frequencies between 4.4 and 7.2 Hz. The likelihood that tremor would occur increased with increasing stimulus intensities or frequencies. It was maximal with stimulation over the forearm area, and decreased with stimulation over the leg area, or over parietal sites; there was no tremor during stimulation of cervical nerve roots. The frequency of the induced tremor was independent of the rate of stimulation and did not depend on the presence of excitatory and inhibitory motor responses to the stimulus. Stimulation could also induce tremor of the same frequency in the fixed task, but only during co-contraction of forearm muscles. The amplitude of tremor was proportional to the level of co-contraction. Clinically, the tremor induced by repetitive TMS appeared very similar to cerebellar tremors. In order to confirm this we investigated two cerebellar patients, one with autosomal dominant cerebellar ataxia and the other with multiple sclerosis. Both of them had a terminal tremor of 6-7 Hz in the wrist movement task. In the holding task, the amplitude of their postural tremor increased with the level of co-contraction in forearm muscles. Since the frequency of repetitive TMS-induced tremor was independent of stimulus parameters, we conclude that it represents some intrinsic property of the CNS. We suggest that the tremor is caused by disruption of cortical processes involved in terminating a voluntary movement or maintaining a posture. Similarities to cerebellar patients suggest that repetitive TMS may cause tremor by interfering with adaptive cerebellar afferent inflow to motor cortex. Repetitive TMS-induced tremor, therefore, may represent a model of some forms of cerebellar tremor in man.
Synthesis and Characterization of Porous Magnesium Hydroxide and Oxide Nanoplates (2003)

Porous magnesium hydroxide nanoplates were prepared directly from commercial bulk magnesium oxide crystals by a simple hydrothermal treatment. These thin plates would aggregate into large spherical particles. The platelike morphology was retained after calcination, and porous magnesium oxide nanoplates were obtained. These plates have a wormhole-like porous structure with high surface area. The obtained materials exhibit bimodal pore size distributions in the mesoporous domain. The aggregation of the nanoplates gives rise to large mesopores with a size of about 36 nm. In addition, each plate has small wormhole mesopores with a size of about 3.7 nm. The growth of magnesium hydroxide nanoplates occurred through a dissolution−recrystallization process. X-ray diffraction and electron diffraction, transmission electron microscopy, scanning electron microscopy, and nitrogen sorption have been employed to characterize these nanoplates. Such porous nanoplates with high surface area and high crystallinity have many promising applications. Moreover, bismuth oxide nanoplates were also produced following a similar method. The formation mechanism of such mesostructures without the use of a template is also discussed.
Microbial attack of nitrocellulose (1981)

Abstract This is a study dealing with the use of a microorganism for partial denitration of the surface of nitrocellulose‐based small arms propellants, in order to gain burning rate control. An organism, Aspergillus fumigatus , was found to grow on pyroxylin suspended in a nitrogen deficient, carbon‐containing nutrient medium. No growth was observed under the same conditions when carbon was absent. This would indicate that the organism utilized nitrogen from nitrocellulose without attacking the carbon backbone. Further, mechanism studies indicated that the organism did not utilize the nitrogen directly from nitrocellulose, but rather relied on a hydrolysis reaction for a source of nitrogen.
Polarization of Iron in H2S-NaHS Buffers (1965)

Effects of H2S concentration, pH, and buffer capacity on the hydrogen evolution reaction are reported. Over-all electrode reaction in the presence of H2S is: 2H2S+2e−→H2+2HS− The reaction is limited by H2S diffusion and by hydrogen overvoltage. Above the diffusion current reaction is by decomposition of HS− or H2O. Expressions are derived for calculating the pH at the electrode surface at, above and below the diffusion current. After correcting for pH changes, Tafel slopes of 115 mv are found in 10−3 M HS− and 55-70 mv for higher concentrations of HS− below the diffusion current. Solutions of 10−1 and 10−2 M HS− develop protective films. Anodic polarization curves for these solutions show typical active-passive transitions, with very low residual currents in the passive region.
Analysis and Experimentation of Model Predictive Control for Spacecraft Rendezvous and Proximity Operations with Multiple Obstacle Avoidance (2016)

Presented at AIAA/AAS Astrodynamics Specialist Conference, Long Beach, CA. The article of record as published may be found at https://doi.org/10.2514/6.2016-5269
Calcium-dependent growth regulation of small cell lung cancer cells by neuropeptides (2006)

Approximately 15-25% of all primary cancers of the lung are classified histologically as small cell lung carcinoma (SCLC), a subtype characterized by rapid growth and a poor prognosis. Neuropeptide hormones like bombesin/gastrin-releasing peptide, bradykinin or galanin are the principal mitogenic stimuli of this tumour entity. The mitogenic signal is transmitted into the cell via heptahelical neuropeptide hormone receptors, which couple to the heterotrimeric G proteins of the Gq/11 familiy. Subsequent activation of phospholipase Cbeta (PLCbeta) entails the activation of protein kinase C and the elevation of the intracellular calcium concentration. There is mounting evidence to support the notion that calcium mobilization is the key event that initiates different mitogen-activated protein kinase cascades. Neuropeptide-dependent proliferation of SCLC cells relies on parallel activation of the Gq/11/PLCbeta/Ras/extracellular signal-regulated kinase and the c-jun N-terminal kinase pathways, while selective engagement of either signalling cascade alone results in growth arrest and differentiation or apoptotic cell death. Basic experimental research has the potential to identify and validate novel therapeutic targets located at critical points of convergence of different mitogenic signal transduction pathways. In the case of SCLC, targeting the distinct components of the Ca2+ influx pathway as well as critical Ca2+-dependent cellular effectors may be rewarding in this regard.