gebhart/patrepeval huggingface dataset:
Combination of N149S and D171G mutations in<i>Aeromonas caviae</i>polyhydroxyalkanoate synthase and impact on polyhydroxyalkanoate biosynthesis (2007)

Aeromonas caviae polyhydroxyalkanoate synthase (PhaC(Ac)) is an important biocatalyst for the synthesis of practically useful two-component polyhydroxyalkanoate copolymer, poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)]. In a previous study, two PhaC(Ac) mutants that have a single amino acid substitution of either asparagine 149 by serine (N149S) or aspartate 171 by glycine (D171G) were isolated as higher active enzymes by means of evolutionary engineering. In this study, the synergistic effects of N149S and D171G double mutation (NSDG) in PhaC(Ac) on polyhydroxyalkanoate biosynthesis were investigated in recombinant Ralstonia eutropha. The PhaC(Ac) NSDG mutant showed enhanced incorporation of longer 3-hydroxyalkanoate (3HA) units into the polyhydroxyalkanoate copolymer from octanoate (3HA fraction: 18.5 mol%) and soybean oil (5.4 mol%) as a carbon source. Besides, the NSDG mutant synthesized P(3HB) homopolymer with a very high molecular weight (M(w)=368 x 10(4)) when fructose was used as a carbon source. Thus, a combination of the beneficial mutations synergistically altered enzymatic properties, leading to synthesis of a polyhydroxyalkanoate copolymer with enhanced 3HA fraction and increased molecular weight.
Use of Signal-Mediated Amplification of RNA Technology (SMART) to Detect Marine Cyanophage DNA (2002)

Here, we describe the application of an isothermal nucleic acid amplification assay, signal-mediated amplification of RNA technology (SMART), to detect DNA extracted from marine cyanophages known to infect unicellular cyanobacteria from the genus Synechococcus. The SMART assay is based on the target-dependent production of multiple copies of an RNA signal, which is measured by an enzyme-linked oligosorbent assay. SMART was able to detect both synthetic oligonucleotide targets and genomic cyanophage DNA using probes designed against the portal vertex gene (g20). Specific signals were obtained for each cyanophage strain (S-PM2 and S-BnMI). Nonspecific genomic DNA did not produce false signals or inhibit the detection of a specific target. In addition, we found that extensive purification of target DNA may not be required since signals were obtained from crude cyanophage lysates. This is the first report of the SMART assay being used to discriminate between two similar target sequences.
Comparison of p40 (Δ<scp>N</scp>p63) and p63 expression in prostate tissues – which one is the superior diagnostic marker for basal cells? (2013)

In most cases, p40 stains prostatic basal cells as reliably as p63, with only minor differences. Aberrant staining of tumour cells is seen more rarely than with p63 (clone 4A4), establishing its higher specificity. However, additional cytoplasmic immunoreactivity narrows its eligibility for antibody cocktails (e.g. with alpha-methylacyl-CoA racemase).
Regeneration in Markov Chain Samplers (1995)

Abstract Markov chain sampling has recently received considerable attention, in particular in the context of Bayesian computation and maximum likelihood estimation. This article discusses the use of Markov chain splitting, originally developed for the theoretical analysis of general state-space Markov chains, to introduce regeneration into Markov chain samplers. This allows the use of regenerative methods for analyzing the output of these samplers and can provide a useful diagnostic of sampler performance. The approach is applied to several samplers, including certain Metropolis samplers that can be used on their own or in hybrid samplers, and is illustrated in several examples.
<b>Growth hormone improves cardiac function in rats with experimental myocardial infarction</b> (1997)

Accumulating evidence suggests from experimental and clinical studies beneficial effects of growth hormone (GH) on contractility, although concomitant cardiac hypertrophy, generally considered to be a cardiovascular risk factor, has also been reported. In the present study, we combine a rat model with impaired cardiac performance after myocardial infarction (MI) with echocardiographic evaluation of GH effects on cardiac structure and function. We have used a rat model with ligation of the left coronary artery in normal, growing male rats resulting in subsequent impaired cardiac performance. After 6 weeks' recovery, blind transthoracic echocardiography was performed to determine infarction size, cardiac geometry and performance. Rats with no signs of myocardial infarction were excluded from the study. After randomization, the rats were treated with daily s.c. injections of saline (n = 8) or recombinant human growth hormone (rhGH) (n = 6) at a dose of approximately 1 mg kg-1 body weight for 1 week. A new blind echocardiography examination was performed after treatment demonstrating a 13% increase in ejection fraction (EF) and a 50% increase in cardiac index in GH-treated rats compared with control rats (P < 0.01). Moreover, GH caused a significant decrease in end-systolic volume. There were no significant changes in left ventricular (LV) or interventricular wall thickness, LV dimensions, heart rate or diastolic function. No effects were seen on LV weight, cardiac insulin-like growth factor (IGF) I, IGF-I receptor and GH receptor mRNA content. GH in a physiological dose improves systolic function in an experimental model of heart failure without signs of hypertrophy, suggesting a potential role as a therapeutic agent in the treatment of heart failure and merits further investigation.
Energy losses in conductors carrying very high currents (1981)

Conductors carrying very high currents show losses of an electromagnetic and a shock compression nature. Electromagnetic losses (joule heating in the skin layer, magnetic flux diffusion) scale as H/sup 3/ or (I/a)/sup 3/, where H is the self magnetic field of the current and I/a is the current divided by conductor - periphery; shock losses scale as H/sup 4/ or H/sup 3/ ((I/a)/sup 4/ or (I/a)/sup 3/) depending on the magnitude of I. In experiments where electrical energy must be converged from a large pulsed power supply to a small load, these losses can account for half the orignal energy and limit the magnitude of the energy per unit volume in the load. These considerations may be important for the study of material properties at high energy density.
Photophysics and photochemical reactivities of organocopper(<scp>I</scp>) complexes. Crystal structure of [Cu<sub>2</sub>(PPh<sub>2</sub>Me)<sub>4</sub>(µ,η<sup>1</sup>-CCPh)<sub>2</sub>] (1996)

Dimeric [Cu2(PPh2Me)4(µ,η1-CCPh)2] has been synthesized and crystallographically characterized; its photophysics together with those of other related organocopper(I) complexes, [Cu2{2-C(SiMe3)2C5H4N}2] and [{Cu(C6H2Me3-2,4,6)}5] have been studied and Stern–Volmer quenching data of the phosphorescent state of the pyridine complex with organic halides are suggestive of a charge-transfer mechanism with a high driving-force regime.
Multi-Device Seamless Service by User-Oriented Session in All-IP Network (2007)

Evolutionary development towards all-IP networks will have various kinds of devices connected to the Internet. In such the ubiquitous networking environment with the features of heterogeneity and ubiquity, the user shall be able to communicate by a best suited way according to his situation and preference. In this paper, a user-oriented session is shown that enables a user to switch devices, media streams, and networks flexibly even during a session. Additionally, protocol adaptation technology is also provided so that various types of devices could be connected to the IMS/MMD networks that are fundamental infrastructures of the all-IP networks. We implemented the proposed system as a prototype using a BREW-enabled mobile phone and MMD core network.
An auditory brain-computer communication device (2005)

The thought-translation-device (TTD) is a brain-computer-interface (BCI) that enables completely paralyzed patients to communicate with their brain signals only. Voluntary self-regulation of brain signals (e.g. the slow cortical potentials) is achieved by a feedback training. Visual impairment of these patients requires an auditory feedback mode. The TTD can be entirely operated by listening and voluntary production of specific mental activity. It provides auditory (sonified) feedback of multiple EEG parameters for the training of self-regulation of brain signals which can operate a verbal spelling interface. The properties of the system are reported and the results of experiments with auditory feedback are presented.
Effect of ecdysterone on protein expression of PI3K,Glut-4 in insulin-resistant HepG2 cells (2009)

Objective To investigate the effect of ecdysterone on protein expressions of phosphatidylinositol 3-kinase(PI3K)and glucose transporter 4(GLUT-4)in the insulin-resistant(IR)HepG2 cells.Methods After establishment of the insulin resistant HepG2 cell model induced by high concentration of insulin.The HepG2 cells were incubated with 1×10-5M ecdysterone for 24 h.Immunocytochemical assay was used to assess the expression of PI3K and GLUT-4 was determined by Western blotting.Results The incubation of insulin resistance cells with ecdysterone 1×10-5M could significantly increase the protein expressions of PI3K and GLUT-4 in the IR HepG2 cells(P0.05).Conclusion Ecdysterone could improve the insulin sensitivity in IR HepG2 cells probably by upregulating the expression of insulin signaling proteins such as PI3K and GLUT-4.
Wavefront customized visual corrections : the quest for super vision II (2004)

Wavefront technology has seen an explosion of information and in keeping with the fast-paced changing world is the premier source on correction, Wavefront Customized Visual Correction: The Quest for Super Vision II. Recognized as the premier source on correction, Wavefront Customized Visual Correction details the latest advancements in state-of-the-art wavefront-guided ophthalmic corrections poised to revolutionize the eyecare world in the future. As ophthalmic technology and applications have matured, testing and wavefront-guided corrections are fast becoming ophthalmic prescriptions of unparalleled visual quality. A complete revision to the best-selling Customized Corneal Ablation: The Quest for Super Vision, this wavefront bible incorporates additional forms of customized diagnosis and correction, including intraocular lens correction, presbyopic issues, and contact lenses. New Topics Include: Significant clinical trial results on customized ablation (from the US FDA and international studies); Basic and advanced diagnostics and interpretation. Basic science of sensing and principles of sensing devices. Advanced customization in presbyopic correction. Wavefront customized lenses including IOLs and contact lenses. A section on advanced diagnostics and standards has been rewritten and expanded to account for the latest breakthroughs. This section is critical in properly educating eyecare professionals about this innovative technology. Wavefront Customized Visual Correction has opened the door to a world of future possibilities on the application of technology to the eye and will remain the most comprehensive single source on and customized visual correction.
Embolization of cerebral vessels with inflatable and detachable balloons (1978)

✓ The authors describe an inflatable and detachable rubber balloon for embolization of cerebral or spinal vessels.
Extra‐articular absorbable suture stabilization of coxofemoral luxation in dogs (2001)

Absorbable sutures avoid the possible complications related to the use of nonabsorbable material and seem to be sufficient to maintain articular stability during the capsular healing process.
Base-Catalyzed Rearrangement of Some 1,3-Oxathiolane Sulfoxides: Mechanistic Viewpoint of the Sigmatropic and Elimination Reactions (2004)

Rearrangements of 1,3-oxathiolane sulfoxides 8 and 9 in the presence of base are described from a mechanistic viewpoint of sigmatropic and elimination reactions. In the presence of triethylamine the (Z)-sulfoxide 8 gave the corresponding thiolsulfinate 10 by way of dimerization of the sulfenic acid intermediate 2 at room temperature while the (E)-sulfoxide 9 was recovered even after refluxing in ethyl acetate by the reversal of the [2,3]-sigmatropic rearrangement of the sulfenic acid 4. Triethylamine promoted the developing charge separation in the transition state of the sigmatropic rearrangement of the (Z)-sulfoxide 8 to facilitate the ring opening to the sulfenic acid 2. The reason for more facile ring opening of the (Z)-sulfoxide 8 in comparison with the corresponding (E)-sulfoxide 9 is attributable to the differences in the reactivity of the hydrogen adjacent to the carbonyl group. Triethylamine was not strong base to deprotonate the carbonyl-activated methylene hydrogen of the (E)-sulfoxide 9 but enough to catalyze the sigmatropic process of the sulfoxides. The sulfenic acid 2 dimerized to the thiolsulfinate 10 while the sulfenic acid 4 proceeded the sigmatropic ring closure. In the presence of strong base such as potassium hydroxide, the elimination reaction was predominant over the sigmatropic rearrangement. In this reaction condition, both sulfoxides 8a and 9a gave a mixture of the disulfide 12, the isomeric disulfide 14, and the sulfinic acid 13. Under the strong alkaline condition an elimination of activated hydrogen from the carbon adjacent to the carbonyl group to furnish the sulfenic acid 2a and the isomeric sulfenic acid 18. The formation of the transient intermediate in the reaction was proven by isolation of the isomeric disulfide 14. The reactive entity was regarded as the sulfenic acid rather than sulfenate anion under these reaction conditions.
La Crosse Encephalitis in Children (2001)

La Crosse virus infection should be considered in children who present with aseptic meningitis or encephalitis. Hyponatremia and increasing body temperature may be related to clinical deterioration.
Specific destruction of host-reactive mature T cells of donor origin prevents graft-versus-host disease in cyclophosphamide-induced tolerant mice (1991)

In cyclophosphamide (CP)-induced tolerance, a long lasting skin allograft tolerance was established in many H-2-identical strain combinations without graft vs host disease. Destruction of donor-reactive T cells of host origin, followed by intrathymic clonal deletion of these cells, has been revealed to be the chief mechanisms of this system. Here, we studied the fate of host-reactive populations in donor-derived T cells of C3H/He (C3H) (H-2k, Mls-1b, Mls-2a) mice rendered CP-induced tolerant to AKR/J (AKR) (H-2k, Mls-1a, Mls-2b), by assessing AKR-derived Thy-1.1+ T cells bearing TCR V beta 3 that are specifically reactive with Mls-2a-encoded Ag of the recipient C3H mice. In the AKR-derived Thy-1.1+ lymph node cells of the C3H mice that had been treated with AKR spleen cells plus CP, CD4(+)-V beta 3+ T cells were obviously decreased by day 10 after the CP treatment. At this stage, the Thy-1.1+ T cells were not detected in the C3H thymus, suggesting that the obvious decrease of CD4(+)-V beta 3+ T cells of AKR origin was not due to intrathymic clonal deletion in the recipient C3H mice. Therefore, the destruction of the host-reactive mature T cells of donor origin, as well as that of the donor-reactive mature T cells of host origin, occurred by the CP treatment at the induction phase. Furthermore, after the establishment of intrathymic mixed chimerism in the recipient C3H mice, V beta 3+ T cells were not detected among the Thy-1.1+ T cells of AKR origin in the mixed chimeric thymus, suggesting that the host-reactive immature T cells repopulated from the injected donor hematopoietic cells were clonally deleted in the recipient thymus. These two mechanisms appear to prevent graft vs host disease in CP-induced tolerance.
Global Gene Expression responses to 16-week High Fat Diet in Skeletal Muscle of C57BL/6 Mice (2010)

The expression of multiple genes can be measured simultaneously by gene microarray. Compared with traditional biological technology, gene array represents a simple, fast and high flux. It has been well recognized that human dietary habits have changed in the past decades and long-term high-fat diet can cause energy imbalance between intake and consumption, which could cause the abnormal signal pathways and promote the development of metabolic disorders such as obesity, insulin resistance, and type 2 diabetes. The high-fat diet is increasingly recognized as an important cause for metabolism syndrome. PURPOSE: To study the molecule mechanisms in the development of insulin resistance induced by long-term high-fat diet, gene microarray was performed to analyze the changes of global gene expression in the skeletal muscle of C57BL/6 mice. The differentially expressed genes were analyzed to provide the experimental evidences and potential targets for the cure of metabolic disorders. METHODS: 20 of 4-week old, C57BL/6 male mice were randomly divided into two groups, normal diet group (C) and high fat diet group (H), and then the mice were feed by normal chow or high fat diet for 16 weeks respectively. Six mice were randomly selected from each group and skeletal muscle was removed for RNA extraction. The RNA samples were then analyzed by gene microarray and data were evaluated by statistics software SPSS. Significance Analysis of Microarrays Software provided by Capital-Bio Corporation was used to dissect the differentially expressed genes between two groups. RESULTS: There are 590 differentially expressed genes in group H comparing with group C after 16 -week high-fat diet, including 321 up-regulated and 269 down-regulated genes. The 590 differentially expressed genes were further analyzed by gene ontology (GO) and the results indicated that these genes play 727 different molecular functions, participate 412 biological processes and 229 cellular component. The differentially expressed genes between the C and H groups were further tested by Disease Categorization and the result showed 10 genes intimately correlated with T2DM, including IDE,CASQ1,APOE,APOC3,PDK4,KLF13,AKT1,UCP2,GSTT1 and ADM. Furthermore, APOE,APOC3 and UCP2 were also responsible for the cause of other diseases including hypertension, atherosclerosis, obesity and liver disease. CONCLUSION: Long-term high-fat diet can produce significant changes in global gene expression in the skeletal muscle of C57BL/6 mice.
Acute hydrogen sulfide poisoning in a dairy farmer (2007)

Hydrogen sulfide is a significant occupational health hazard. Education, personal protective equipment, and early treatment are important in improving outcomes.
High-Energy Transurethral Thermotherapy with CoreTherm Approaches Transurethral Prostate Resection in Outcome Efficacy: A Meta-Analysis (2008)

A meta-analysis of current randomized controlled trials that compared TURP with HE-TUMT demonstrates more significant changes in Q(max), IPSS, and PVR when TURP is used to manage symptomatic BPH. Despite these statistical differences, stratified data demonstrate that current HE-TUMT machinery is more effective than previously used lower-energy machinery, especially at objective end points. This is most evident when the CoreTherm device is used. These findings, coupled with the decreased costs and morbidity associated with HE-TUMT, support this treatment as a reasonable alternative to TURP.
A novel generic fast Fourier transform pruning technique and complexity analysis (2004)

The fast Fourier transform (FFT) is an essential tool in digital signal processing and communications. In the applications of the FFT where the required outputs are very sparse, for example, in digital filtering, one may only require the spectrum corresponding to certain bins of the FFT or in narrow frequency windows. In these cases, most of the FFT outputs are not required. Some pruning algorithms have been proposed to deal with such cases. However, most of the pruning algorithms require that the outputs be in continuous windows. This paper develops a traced FFT Pruning method (TFFTP), which is a novel technique and does not require this condition. Under some circumstances, considerable savings in computational complexity and power consumption can be realized using the TFFTP compared to the FFT. This paper derives the average number of butterflies that need to be executed when only k/sub in/ input or/and k/sub out/ output bins of an N point FFT, where k/sub in//spl les/N, or/and k/sub out//spl les/N are required. This method is then extended to arbitrary radix FFT pruning and simultaneous input and output pruning case.
A Bidirectional Relationship Between Psychosocial Factors and Atopic Disorders: A Systematic Review and Meta-Analysis (2007)

The current review revealed a robust relationship between psychosocial factors and atopic disorders. This supports the use of psychological in addition to conventional physical and pharmacological interventions, in the successful prevention and management of atopic disorders.
Intramolecular electronic energy transfer between rigidly linked naphthalene and anthracene chromophores (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIntramolecular electronic energy transfer between rigidly linked naphthalene and anthracene chromophoresGregory D. Scholes, Kenneth P. Ghiggino, Anna M. Oliver, and Michael N. Paddon-RowCite this: J. Phys. Chem. 1993, 97, 46, 11871–11876Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/j100148a006https://doi.org/10.1021/j100148a006research-articleACS PublicationsRequest reuse permissionsArticle Views728Altmetric-Citations90LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts