gebhart/patrepeval huggingface dataset:
High-Speed Digital Golay Code Flaw Detection System (1981)

Current ultrasonic c orrelation flau detection systems are constrained to slow operation speed because long correlation times are required to reduce self-noise to tolerable levels. Self-noise occurs due to incomplete pulse-compression in the correlation detector and causes interference uhen a flau is located next to a large surface or in a large grained material. A neu, entirely digital, flau detection system is described uhich uses paired pseudo-random codes called complementary Golay codes, as the transmit signals, to cancel self-noise through correlation of tu0 transmit bursts. Results are presented uhich shou that the Golay code system uill produce better performance in the presence of g rains and large reflecting surfaces, and can operate at much higher speed than previous correlation flau detection systems. The neu Golay code system is also show to have the same resolution as conventional pulse-echo systems and to obtain the same high signal-tonoise ratio enhancement as in previously described random signal correlation flaw detection systems.
BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5 (2015)

The transcription factor KLF5 is highly expressed in basal-like breast cancer and promotes breast cancer cell proliferation, survival, migration and tumour growth. Here we show that, in breast cancer cells, KLF5 is stabilized by the deubiquitinase (DUB) BAP1. With a genome-wide siRNA library screen of DUBs, we identify BAP1 as a bona fide KLF5 DUB. BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination. KLF5 is in the BAP1/HCF-1 complex, and this newly identified complex promotes cell cycle progression partially by inhibiting p27 gene expression. Furthermore, BAP1 knockdown inhibits tumorigenicity and lung metastasis, which can be rescued partially by ectopic expression of KLF5. Collectively, our findings not only identify BAP1 as the DUB for KLF5, but also reveal a critical mechanism that regulates KLF5 expression in breast cancer. Our findings indicate that BAP1 could be a potential therapeutic target for breast and other cancers.
High Efficiency Retroviral-Mediated Gene Transduction into CD34 <sup>+</sup> Cells Purified from Peripheral Blood of Breast Cancer Patients Primed with Chemotherapy and Granulocyte-Macrophage Colony-Stimulating Factor (1994)

The hematopoietic system has been one of the major targets for designing human gene therapy protocols. In the present system, we have transduced LNL6, one of the most commonly used retroviral vectors in gene therapy, into purified CD34+ cells from peripheral blood of patients primed with chemotherapy and granulocyte-macrophage colony-stimulating factor (GM-CSF). Purification of CD34+ cells was achieved by incubation with a murine anti-CD34 monoclonal antibody (9C5), and subsequently with paramagnetic microspheres (Dynal) coated with sheep anti-mouse IgG1 (Fc). The CD34+ cells were released from the beads by treatment with chymopapain. Flow cytometry analysis using the anti-CD34 antibody HPCA-2-FITC targeted at another epitope of CD34 showed that 78-97.5% of the cells thus purified were CD34+. After retroviral-mediated gene transfer, polymerase chain reaction (PCR) analysis revealed that 67-100% of the hematopoietic colonies contained the marker gene neo, indicating that CD34+ cells purified by immunomagnetic microsphere method from peripheral mononuclear cells primed with hematopoietic growth factors are highly susceptible to retroviral-mediated gene transfer. The expression of neo as determined by reverse transcription (RT)-PCR appeared to be unstable and not persistent. Taken together, our data suggest that LNL6 is a suitable vector for gene marking of hematopoietic progenitors but not for gene therapy protocols based on persistent gene expression.
Diamond power FET concept (2002)

In this paper a novel concept for diamond power FETs is presented. This concept is based on a /spl delta/-doped active channel using homoepitaxial CVD-layers on 100-oriented single crystals. The channel is controlled by a recessed sub-/spl mu/m pn-junction gate. Based on technological building blocks developed previously, the structure has been simulated and 13 W/mm RF power are predicted. First fabricated FETs show that the concept is feasible.
Elucidating Double Aggregation Mechanisms in the Morphology Optimization of Diketopyrrolopyrrole‐Based Narrow Bandgap Polymer Solar Cells (2014)

The power conversion efficiency (PCE) of a DPP-based polymer solar cell is significantly improved by using DIO or DCB as processing additives. The discovery that DCB outperforms DIO with a significantly wider solvent mixture operation window suggests different optimization mechanisms. Although both solvent mixture systems involve double aggregation processes, including a similar solution-to-film aggregation, however, two distinct solution-stage aggregations are observed: relatively amorphous polymer aggregates form in the CF-DIO solution, while more crystalline polymer aggregates form in CF-DCB solution.
Spatial-geometric approach to physical mobile interaction based on accelerometer and IR sensory data fusion (2010)

Interaction with the physical environment using mobile phones has become increasingly desirable and feasible. Nowadays mobile phones are being used to control different devices and access information/services related to those devices. To facilitate such interaction, devices are usually marked with RFID tags or visual markers, which are read by a mobile phone equipped with an integrated RFID reader or camera to fetch related information about those objects and initiate further actions. This article contributes in this domain of mobile physical interaction; however, using a spatial-geometric approach for interacting with indoor physical objects and artifacts instead of RFID based solutions. Using this approach, a mobile phone can point from a distance to an annotated object or a spatial subregion of that object for the purpose of interaction. The pointing direction and location is determined based on the fusion of IR camera and accelerometer data, where the IR cameras are used to calculate the 3D position of the mobile phone users and the accelerometer in the phone provides its tilting and orientation information. The annotation of objects and their subregions with which the mobile phone interacts is performed by specifying their geometric coordinates and associating related information or services with them. We perform experiment in a technology-augmented smart space and show the applicability and potential of the proposed approach.
The Development of Professional Standards in Coaching (2014)

Создание профессиональных стандартов коучингаАвтор утверждает, что мы достигли той точки, когда необходимо создать некоторое общее понимание коучинга для того, чтобы защитить профессию, репутацию профессиональных коучей и интересы их клиентов.
Real-Time Quantitative PCR for the Detection of Fetal Aneuploidies (2008)

In prenatal analysis, one of the major concerns is the detection of fetal aneuploidies. Several molecular methods have been described recently for the rapid analysis of amniotic fluid and chorionic villi. Fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) of short tandem repeats are already implemented in prenatal laboratories and permit the evaluation of the major chromosomal aberrations within 24 h. However, both methods have their disadvantages. The advent of real-time PCR has revolutionized the measurement of nucleic acid copy numbers in recent years. Quantitative PCR, a term that was only 10 years ago considered to be an oxymoron, is now widely accepted. We demonstrated previously the feasibility of detection of trisomy 21 by real-time PCR, and here we describe the modified test that permits simultaneous analysis of trisomies 18 and 21. This approach has been demonstrated in a recent large-scale analysis, and it is presented in detail in this chapter.
Engineering and rapid selection of a low‐affinity glucose/galactose‐binding protein for a glucose biosensor (2007)

Periplasmic expression screening is a selection technique used to enrich high-affinity proteins in Escherichia coli. We report using this screening method to rapidly select a mutated D-glucose/D-galactose-binding protein (GGBP) having low affinity to glucose. Wild-type GGBP has an equilibrium dissociation constant of 0.2 microM and mediates the transport of glucose within the periplasm of E. coli. The protein undergoes a large conformational change on binding glucose and, when labeled with an environmentally sensitive fluorophore, GGBP can relay glucose concentrations, making it of potential interest as a biosensor for diabetics. This use necessitates altering the glucose affinity of GGBP, bringing it into the physiologically relevant range for monitoring glucose in humans (1.7-33 mM). To accomplish this a focused library was constructed using structure-based site-saturation mutagenesis to randomize amino acids in the binding pocket of GGBP at or near direct H-bonding sites and screening the library within the bacterial periplasm. After selection, equilibrium dissociation constants were confirmed by glucose titration and fluorescence monitoring of purified mutants labeled site-specifically at E149C with the fluorophore IANBD (N,N'-dimethyl-N-(iodoacetyl)-N'-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylene-diamine). The screening identified a single mutation A213R that lowers GGBP glucose affinity 5000-fold to 1 mM. Computational modeling suggested the large decrease in affinity was accomplished by the arginine side chain perturbing H-bonding and increasing the entropic barrier to the closed conformation. Overall, these experiments demonstrate the ability of structure-based site-saturation mutagenesis and periplasmic expression screening to discover low-affinity GGBP mutants having potential utility for measuring glucose in humans.
Mobility enhancement design and control for a Dual-Wheel Dual-Armed Mobile Manipulator (2015)

Recently domestic Wheeled Mobile Robot(WMR) such as "iRobot Roomba Vacuum Cleaning Robot" is becoming popular. However, such WMR has poor mobility to surmount the obstacles like the door sill, which greatly limits its functionality. Instead of using complex wheel mechanisms, another possible solution is to use external manipulator. Mobile Manipulator(MM) is a robotic system combing the mobile robot and manipulator, hence inherits their natural advantages. Most researches payed attention to enhancing manipulability instead of enhancing the mobility of the hybrid mobile manipulator. In this paper we propose to use two manipulators to enhance the mobility of a WMR-Roomba to surmount the obstacle. The mobile manipulators can work in two different modes. When the mobile manipulator moves on flat ground, wheels are the main driving unit and can achieve high motion efficiency; When the WMR encounters the obstacle, manipulators are the main driving unit and can achieve high mobility. The design and control procedure of the Dual-Wheel Dual-Armed Mobile Manipulator(DWDAMM) is presented. By analyzing the kinematics of the Mobile Manipulators and using cubic polynomial interpolation method, the parameter design and trajectory planning problem are turned into an optimization problem. Simulation experiments were performed using MATLAB and SimMechanics. Results show that the proposed design and control method are effective and can enhance mobility of the original wheeled mobile robot.
Stabilization and Augmentation of Circulating AIM in Mice by Synthesized IgM-Fc (2014)

Owing to rapid and drastic changes in lifestyle and eating habits in modern society, obesity and obesity-associated diseases are among the most important public health problems. Hence, the development of therapeutic approaches to regulate obesity is strongly desired. In view of previous work showing that apoptosis inhibitor of macrophage (AIM) blocks lipid storage in adipocytes, thereby preventing obesity caused by a high-fat diet, we here explored a strategy to augment circulating AIM levels. We synthesized the Fc portion of the soluble human immunoglobulin (Ig)M heavy chain and found that it formed a pentamer containing IgJ as natural IgM does, and effectively associated with AIM in vitro. When we injected the synthesized Fc intravenously into mice lacking circulating IgM, it associated with endogenous mouse AIM, protecting AIM from renal excretion and preserving the circulating AIM levels. As the synthesized Fc lacked the antigen-recognizing variable region, it provoked no undesired immune response. In addition, a challenge with the Fc-human AIM complex in wild-type mice, which exhibited normal levels of circulating IgM and AIM, successfully maintained the levels of the human AIM in mouse blood. We also observed that the human AIM was effectively incorporated into adipocytes in visceral fat tissue, suggesting its functionality against obesity. Thus, our findings reveal potent strategies to safely increase AIM levels, which could form the basis for developing novel therapies for obesity.
Defining the unknown: Molecular methods for finding new microbes (1996)

The purpose of this article is to describe specific applications of molecular diagnostics that are currently identifying suspected or unidentified microbial pathogens. The techniques reviewed include (i) the use of specific primers and PCR to identify new microbes, (ii) PCR amplification of conserved 16S rRNA sequence with subsequent identification of specific internal sequence from the candidate bacterial pathogen, and (iii) an exciting new modification of PCR called RDA or "reverse PCR" that can identify unique infectious agents in diseased tissue. The field will continue to expand rapidly and, it is hoped, contribute to a better understanding of the microbial environment with which humans coexist. Also, molecular techniques will eventually be applied in the demonstration of pathogenesis by the various newly identified microbial pathogens.
Cobalt Hydroxide as a Capacitor Material: Tuning Its Potential Window (2008)

Research activities on various supercapacitor materials have grown enormously in recent years. Capacitor studies of over a limited potential window in alkaline electrolytes are reported. A high potential window of a capacitor material is desirable for use in a device. Furthermore, as the specific energy and specific power of a capacitor depend strongly on its voltage, experiments are conducted in the present work to understand the reason for a low potential window for as a capacitor material and also to increase its potential window. Experiments are conducted in NaOH and electrolytes of various concentrations using electrochemically precipitated on stainless steel current collectors in an aqueous electrolyte. Based on the potential window, specific capacitance, and specific energy, it is found that NaOH electrolyte is more appropriate for capacitor properties of than the rest of the electrolytes studied. Using a electrode with a specific mass of in NaOH, a specific capacitance of about is obtained with a potential window of at a charge–discharge current density of .
The Nexus between Traditional and Non-Traditional Security Cooperation in Japan -China Relations: Environmental Security and the Construction of a Northeast Asian Region (2010)

The Ministry of Education, Culture, Sports, Science, and Technology began in 2007 the Global COE (Center of Excellence) Program with the primary aim of developing creative human resources to lead the world and internationally competitive universities in Japan.The Global Institute for Asian Regional Integration (GIARI) of the Graduate School of Asia-Pacifi c Studies, Waseda University is one of the twelve sites in interdisciplinary, combined, and new fi elds selected from among numerous applicants throughout the country.The Global COE program at GIARI has two aims: to develop competent professionals who will contribute to regional cooperation and consolidation, and to build a center for this purpose.The program has three areas of study: (1) political integration and identity; (2) economic integration and sustainability; and (3) social integration and network, and the three areas are organically interconnected.The program is building a theoretical framework for regional governance, allowing Ph.D. candidates to participate in different projects to develop multidimensional and comprehensive perspectives, and has already produced many results in this endeavor.The program also encourages research and other activities to create strong networks with other institutions of higher learning in the region and also to collaborate with government agencies, public organizations, and NGOs in order to build a world-class research center at Waseda University.
Preparation of Diblock and Triblock Copolymers of Styrene, 2,5-Norbornadiene, Ethylmethacrylate and PEG by Nitroxide-controlled Free Radical Polymerization (2001)

2,5-Norbomadiene has been polymerized in bulk at 125 &apos;C in presence of a low molar mass of polystyrene-TEMPO as macroinitiator. The structure and poly-dispersity of the obtained diblock copolymer (PSt-PNB-TEMPO) were determin-ed by IR, &apos; H NMR spectroscopy and GPC measurement, respectively, where TEMPO is 2,2,6,6 tetramethylpiperidinyl-l-oxy and PNB is polynorbomadiene. The triblock copolymer of styrene-2,5-norbomadiene-ethylmethacrylate (PSt-PNB-PEMA) using a PSt-PNB-TEMPO as the macroinitiator in the presence of camphorsulphonic acid (CSA) was prepared. Also the triblock copolymer containing polyethylmethacrylate and poly(ethylene glycol) designated as PEMA-PEG-PEMA was synthesized by a novel method. The &apos; H NMR and FTIR studies of triblock copolymers confirmed their structures and the absence of TEMPO end groups for PEMA. Key Words: diblock and triblock copolymer, synthesis, 2,5-norbomadiene, polystyrene-TEMPO, living radical polymerization
The role of complement C3 opsonization, C5a receptor, and CD14 in<i>E. coli</i>-induced up-regulation of granulocyte and monocyte CD11b/CD18 (CR3), phagocytosis, and oxidative burst in human whole blood (2007)

The relative role of complement and CD14 in Escherichia coli-induced leukocyte CD11b up-regulation, phagocytosis, and oxidative burst in human whole blood was examined. The highly specific thrombin inhibitor lepirudin was used as anticoagulant, as it does not affect complement activation. Complement inhibition at the level of C3 (anti-C2 and anti-factor D) and C5 (C5a receptor antagonist and anti-C5/C5a) efficiently inhibited CD11b up-regulation, phagocytosis, and oxidative burst in granulocytes. Monocyte activation was generally less complement-dependent, but when C3 activation was blocked, a pronounced inhibition of phagocytosis and oxidative burst was obtained. Only the combination of anti-C2 and antifactor D blocked E. coli C3 opsonization completely. Whole E. coli, disrupted E. coli, and the C3-convertase activator cobra venom factor up-regulated CD11b rapidly on both cell types, proportional to their complement activation potential in the fluid phase. In comparison, purified LPS at concentrations comparable with that present in the E. coli preparations did not activate complement. Oxidative burst was induced only by whole bacteria. Finally, the combination of complement inhibition and anti-CD14 completely blocked E. coli-induced granulocyte and monocyte CD11b up-regulation and quantitatively, virtually abolished phagocytosis. The results indicate that complement and CD14, despite differential effects on granulocytes and monocytes, are the two crucial, quantitative factors responsible for E. coli-induced CD11b, phagocytosis, and oxidative burst in both cell types.
Reflectometric fiber optic frequency-modulated continuous-wave interferometric displacement sensor (2005)

A practical reflectometric fiber optic displacement sensor is reported. The sensor is based on the principle of optical frequency-modulated continuous-wave (FMCW) interference and primarily consists of a single-mode laser diode, a Y-type fiber optic directional coupler, and a quarter-pitch gradient-index lens. The displacement of the target is determined by measuring the phase shift of the beat signal with a special signal-processing method. An accuracy of 0.02 µm in a measurement range of 1000 µm has been achieved.
Automatic generation of fast optimizing code generators (1988)

article Automatic generation of fast optimizing code generators Share on Authors: C. W. Fraser AT&T Bell Laboratories, Murray Hill, NJ AT&T Bell Laboratories, Murray Hill, NJView Profile , A. L. Wendt Univ. of Arizona, Tucson, AZ Univ. of Arizona, Tucson, AZView Profile Authors Info & Claims ACM SIGPLAN NoticesVolume 23Issue 7July 1988 pp 79–84https://doi.org/10.1145/960116.53998Online:01 June 1988Publication History 17citation450DownloadsMetricsTotal Citations17Total Downloads450Last 12 Months5Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Basement Membrane and Collagen Deposition After Laser Subepithelial Keratomileusis and Photorefractive Keratectomy in the Leghorn Chick Eye (2006)

An advantage of LASEK over PRK is the reduction of postoperative haze.
Biodetoxification of silver-cyanide from electroplating industry wastewater (2000)

A bacterial consortium capable of utilizing metal-cyanides as a source of nitrogen was used to develop a microbiological process for the detoxification of silver-cyanide from electroplating wastewater. When the treatment was carried out in a 27-l rotating biological contactor (R3C) in continuous mode, the system could achieve > 99.5% removal of 0.1 mmol l(-1) silver-cyanide (approximately 5 mg l(-1) cyanide and 10 mg l(-1) silver) in 10 h with sugarcane molasses (0.1 ml l(-1)) as carbon source. The silver ions set free during biodegradation were efficiently adsorbed by the bacterial biomass. The RBC-treated effluent was found to be safe for discharge into the environment, as confirmed by chemical analysis and fish bioassay studies.
Evidence of pestivirus RNA in human virus vaccines (1994)

We examined live virus vaccines against measles, mumps, and rubella for the presence of pestivirus RNA or of pestiviruses by reverse transcription PCR. Pestivirus RNA was detected in two measles-mumps-rubella combined vaccines and in two monovalent vaccines against mumps and rubella. Nucleotide sequence analysis of the PCR products indicated that a modified live vaccine strain used for immunization of cattle against bovine viral diarrhea is not responsible for the contamination of the vaccines.
Effect of freezing on the in vivo recovery of irradiated red cells (1994)

Irradiated red cells can be frozen after being stored under various conditions and can still meet established guidelines requiring 75-percent recovery 24 hours after transfusion.
High-Frequency Active Metamaterials (2009)

We present a systematic numerical study of metamaterials integrated with gain media to achieve composite metamaterials at terahertz and infrared wavelength with reduced losses. The impact of spatial dispersion on the effective permeability resonance restoration is emphasized.