gebhart/patrepeval huggingface dataset:
ACTION SPECTRA AGAIN?* (1991)

Action spectroscopy has a long history and is of central importance to photobiological studies. Action spectra were among the first assays to point to chlorophyll as the molecule most responsible for plant growth and to DNA as the genetic material. It is useful to construct action spectra early in the investigation of new areas of photobiological research in an attempt to determine the wavelength limits of the radiation region causing the studied response. But due to the severe absorption of ultraviolet (UV) radiation by biological samples, UV action spectra were first limited to small cells (bacteria and fungi). Advances in techniques (e.g. single cell culture) and analysis allowed accurate action spectra to be reported even for mammalian cells. But precise analytical action spectra are often difficult to obtain when large, pigmented, or groups of cells are investigated. Here some action spectra are limited in interpretation and merely supply a wavelength vs effect curve. When polychromatic sources are employed, the interpretation of action spectra is even more complex and formidable. But such polychromatic action spectra can be more directly related to ambient responses. Since precise action spectra usually require the completion of a relatively large number of careful experiments using somewhat sophisticated equipment over a range of at least six wavelengths, they are often not pursued. But they remain central to the elucidation of the effect being studied. The worldwide community has agreed that stratospheric ozone is depleting, with the possibility of a consequent rise in the amount of UV-B (290-320 nm) reaching the earth's surface. It is therefore essential that new action spectra be completed for UV-B effects on a large variety of responses of human, animal, and aquatic plant systems. Combining these action spectra with the known amounts of UV-B reaching the biosphere can give rise to solar UV effectiveness spectra that, in turn, can give rise to estimates of effect. Preliminary estimates suggest that ozone layer depletion may seriously impact such important biological end-points as skin cancer, cataracts, the immune system, crop yields, and oceanic phytoplankton. So action spectra continue to play a central role in important photobiological research.
Stress Effect of Antilymphocyte Serum (1969)

Antilymphocyte serum prepared in the rabbit against guinea-pig lymph node cells produces a sustained weight loss when injected into normal guinea-pigs over 14 days, compared with a very transient loss produced by normal rabbit serum. Antilymphocyte serum also produces a much greater increase in plasma cortisol level than normal rabbit serum. Part of this effect may be due to adrenal stimulation, and further studies of its activity in adrenalectomized animals are suggested.
Swedish government : article for the magazine for public administration in Leiden (2003)

Since I work at the Ministry of Justice in Sweden, with issues on supervising the Swedish police, I very much enjoyed 1 1king part in the course Public governance and public management ir transition: European perspectives at the Faculty of Social Sciences at Leiden university in May.
Role of “old” pharmacological agents in the treatment of Cushing’s syndrome (2016)

Despite recent advances in the management of endogenous Cushing's syndrome (CS), its treatment remains a challenge. When surgery has been unsuccessful or unfeasible as well in case of recurrence, the "old" pharmacological agents represent an important alternative for both ACTH-dependent and independent hypercortisolism. Especially in the latter, the advent of novel molecules directly targeting ACTH secretion has not outweighed the "old" drugs, which continue to be largely employed and have recently undergone a reappraisal. This review provides a survey of the "old" pharmacological agents in the treatment of CS.
Measuring translational, rotational, and vibrational dynamics in colloids with digital holographic microscopy (2011)

We discuss a new method for simultaneously probing translational, rotational, and vibrational dynamics in dilute colloidal suspensions using digital holographic microscopy (DHM). We record digital holograms of clusters of 1-μm-diameter colloidal spheres interacting through short-range attractions, and we fit the holograms to an exact model of the scattering from multiple spheres. The model, based on the T-matrix formulation, accounts for multiple scattering and near-field coupling. We also explicitly account for the non-asymptotic radial decay of the scattered fields, allowing us to accurately fit holograms recorded with the focal plane located as little as 15 μm from the particle. Applying the fitting technique to a time-series of holograms of Brownian dimers allows simultaneous measurement of six dynamical modes - three translational, two rotational, and one vibrational - on timescales ranging from 10(-3) to 1 s. We measure the translational and rotational diffusion constants to a precision of 0.6%, and we use the vibrational data to measure the interaction potential between the spheres to a precision of ∼50 nm in separation distance. Finally, we show that the fitting technique can be used to measure dynamics of clusters containing three or more spheres.
Generation of insulin‐secreting cells from human embryonic stem cells (2006)

A growth factor-mediated selection method was used to obtained insulin-secreting cells from human embryonic stem cells (hESC; Royan H1). Our resultant cells were positive for dithizone, a zinc-chelating agent known to selectively stain pancreatic beta cells and immunoreactive for antibodies against insulin, glucagon, and C-peptide. Semi-quantitative reverse transcription-polymerase chain reaction detected expression of proinsulin, insulin and other pancreatic beta-cell-related genes, such as Nkx6.1, Is11, Glut2, Pax4, and prohormone convertase2 (PC2). Moreover, glucagon, somatostatin, K(ATP)-channel genes KIR6.2 and SUR1, islet amyloid polypeptide (IAPP), PC1/3, and glucokinase (GCK) were expressed in the differentiating hESC in a developmental stage-dependent manner. Also, the addition of glucose to the culture medium triggered insulin release from differentiated cells, but transmission electron microscopy of the differentiated cells did not show typical beta-cell granules, even though secretary granules were detected. The results showed that hESC have the ability to transcribe and process insulin, but further improvements of the current method are required to generate a sufficient source of true beta cells for the treatment of diabetes mellitus.
Coherent methods in the X-ray sciences (2009)

X-ray sources are developing rapidly and their coherent output is growing correspondingly. The increased coherent flux from modern X-ray sources is being matched with an associated development in experimental methods. This article reviews the literature describing the ideas that utilize the increased brilliance from modern X-ray sources. It explores how ideas in coherent X-ray science are leading to developments in other areas, and vice versa. The article describes measurements of coherence properties and uses this discussion as a base from which to describe partially coherent diffraction and X-ray phase-contrast imaging, with applications in materials science, engineering and medicine. Coherent diffraction imaging methods are reviewed along with associated experiments in materials science. Proposals for experiments to be performed with the new X-ray free-electron lasers are briefly discussed. The literature on X-ray photon-correlation spectroscopy is described and the features it has in common with other coherent X-ray methods are identified. Many of the ideas used in the coherent X-ray literature have their origins in the optical and electron communities and these connections are explored. A review of the areas in which ideas from coherent X-ray methods are contributing to methods for the neutron, electron and optical communities is presented.
Winglet Design and Optimization for UAVs (2010)

Winglets are known improve the efficiency of large aircraft at high subsonic speeds, but winglet designs for smaller aircraft such as UAVs are largely unproven. Winglets improve efficiency by diffusing the shed wingtip vortex, which in turn reduces the drag due to lift and improves the wing’s lift over drag ratio. This research investigates methods for designing and optimizing winglet geometry for UAVs that operate at Reynolds numbers near 10. The design methodology is based on the vortex lattice method. Optimized designs are tested and compared with base designs for validation and include both Whitcomb and blended winglets. Designs are validated using wind tunnel tests. The resulting methodology is then applied to existing UAV platforms for specific performance improvements.
Microvascular Surgery (1976)

Methods of anastomosing small blood vessels are presented, together with an account of the authors' own experiences in the field of microvascular surgery. Practical suggestions are made to avoid pitfalls. The modern literature on experimental and clinical applications of microvascular technique in plastic and reconstructive surgery is reviewed. An extensive bibliography is offered as an aid to the interested reader.
Stereochemistry and Fate of Hydrogen Atoms in the Diol-Dehydratase-Catalyzed Dehydration ofmeso-Butane-2,3-diol (1998)

The transformation of meso-butane-2,3-diol into butan-2-ol by a strain of Lactobacillus brevis occurs through a diol-dehydratase-catalyzed conversion of the diol into butan-2-one, which is then reduced to the secondary alcohol by dehydrogenases. Experiments performed with deuterated meso-butane-2,3-diols showed that the dehydration reaction brings about an inversion of configuration at the (R)-configured C-atom of meso-butane-2,3-diol as a consequence of the substitution of the OH group by a H-atom; at the same time, the H-atom already bound to the (R) C-atom is retained in the resulting methylene group. The H-atom replacing the OH group was assessed to come from the medium since the H-atom at the (S)-configured C-atom was completely lost. By contrast, in the case of the conversion of (RS)-propane-1,2-diol into propan-1-ol under the same fermentation conditions, an extensive H-transfer (ca. 80%) from the primary-alcohol function to the adjacent C-atom was observed. This fact is taken as an indication of different modes in which the two substrates are processed by the enzyme (despite the same stereochemical outcome). A speculative hypothesis is presented to interpret such a dissimilarity.
HD mice: A novel mouse mutant with a specific defect in the generation of CD4<sup>+</sup>T cells (1998)

We have identified a spontaneous mutation in mice, which we term HD for "helper T cell deficient." This mouse is distinguished by the virtual absence of peripheral T cells of the CD4(+)8(-) major histocompatibility complex (MHC) class II-restricted T helper subset due to a specific block in thymic development. The developmental defect is selective for CD4(+)8(-) cells; the maturation of CD4(-)8(+) and gamma delta T cells is normal. The autosomal recessive mutation underlying the HD phenotype is unrelated to MHC class II, since it segregates independently of the MHC class II locus. Moreover, the HD phenotype is not caused by a defect of the CD4 gene. Bone marrow transfer experiments demonstrate that the defect is intrinsic to cells of the hematopoietic lineage, i.e., most likely to developing thymocytes themselves. The frequency of CD4(+)8(low) intermediate cells is markedly increased in HD mice, suggesting that class II-restricted thymocytes are arrested at this stage. This is the first genetic defect of its kind to be described in the mouse and may prove highly informative in understanding the molecular pathways underlying lineage commitment.
Moulting Hormones. LIII. the Synthesis and Biological Activity of Some Ecdysone Analogues (1981)

A number of ecdysone analogues were prepared to study the effect of structural changes on biological activity. It was found that analogues with the 5a-configuration or a 3,5-cyclo structure were inactive, that a 3ß-hydroxy group enhances activity but is not essential for activity, and that 3ß-substituents decrease activity as follows: OMe (60%), OAc (25%) and OEt (10%). The keto diol (3), keto alcohol (9) and amide (36) were found to be highly toxic to mosquito larvae.
Wide-Range Frequency Offset Estimation Algorithm for Optical Coherent Systems Using Training Sequence (2011)

A wide-range frequency offset estimation algorithm is proposed for optical coherent systems, which removes the modulated phase information from the received signal accurately using training sequence instead of traditional <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">M</i> th power operation. A large frequency offset approximating to ±0.5 times the symbol rate can be traced in arbitrary <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">M</i> -ary phase-shift-keying (MPSK) <i xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">M</i> -ary quadrature amplitude modulation (MQAM) systems by the proposed algorithm with the low computational complexity and the negligible training overhead.
Acquired enophthalmos with systemic lupus erythematosus (2015)

Ocular involvement sometimes occurs with systemic lupus erythematosus (SLE) but enophthalmos with SLE is rare. We report a case of enophthalmos with SLE. A 25-year-old male was admitted for two weeks of fever, sore throat, arthralgia, chest pain and right arm weakness with pain. We diagnosed him with SLE with malar rash, arthritis, pleural effusion, proteinuria, leukopenia, positive antinuclear antibody, anti-dsDNA, and lupus anticoagulant. The patient was prescribed high-dose prednisolone and hydroxychloroquine 400 mg. One week after discharge, he complained about a sensation of a sunken right eye. CT showed right enophthalmos, a post-inflammatory change and chronic inflammation. Proteinuria increased to 3.8 g/day after the patient stopped taking prednisolone. Cyclophosphamide therapy was administered for three months without improvement. We decided to restart prednisolone and change cyclophosphamide to mycophenolate mofetil. Proteinuria decreased but enophthalmos remains as of this reporting.
Second-generation antidepressants for depression in adults. (2013)

What are the comparative effectiveness and adverse effects of second-generation antidepressants for treating depression in adults?
从“松下作风”看日本的管理艺术 (1984)

在管理上美国与日本在所有硬性因素(策略、结构、制度)方面非常相似。主要的差别在于软性因素(技巧、作风、人员与最高目标)。美国由于对这四个软性因素认识不足,造成国际竞争能力低落,因为这些因素是任何要想长期获得成功的企业所不可缺少的要素。如果说有了松下幸之助,日本人才有了一位世界级的经营人才,这也许不算是夸大之辞。他一人融合了斯隆(Alfred Sloan,二十年代初美国通用
Short term variability of oxygen saturation during hemodialysis is a warning parameter for hypotension appearance (2008)

Acute hypotension is a frequent complication of hemodialysis. Blood oxygenation may play a role in hypotension and hypoxemia may be considered as a surrogate marker of hemodynamic instability. Continuous, non-invasive monitoring of oxygen saturation (SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) during hemodialysis is now possible, by means of sensors measuring SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> in blood entering the dialyzer. The aim of the present work was to analyze the short-term variability of SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> during hemodialysis in sessions with and without hypotension to correlate the SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> variability to hemodynamic instability. Our preliminary results showed an interesting, yet to be fully understood, role of SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> in anticipating hypotension onset.
Tennis forehand kinematics change as post-impact ball speed is altered (2011)

Peak joint angles and joint angular velocities were evaluated for varying speed forehands in an attempt to better understand what kinematic variables are most closely related to increases in post-impact ball velocity above 50% of maximal effort. High-speed video was used to measure three-dimensional motion for 12 highly skilled tennis players who performed forehands at three different post-impact ball speeds: fast (42.7 +/- 3.8 m/s), medium (32.1 +/- 2.9 m/s), and slow (21.4 +/- 2.0 m/s). Several dominant-side peak joint angles (prior to ball impact) increased as post-impact ball speed increased from slow to fast: wrist extension (16%), trunk rotation (28%), hip flexion (38%), knee flexion (27%), and dorsiflexion (5%). Between the aforementioned peak joint angles and ball impact, dominant-side peak angular velocities increased as ball speed increased from slow to fast: peak wrist flexion (118%), elbow flexion (176%), trunk rotation (99%), hip extension (143%), knee extension (56%), and plantarflexion (87%). Most kinematic variables changed as forehand ball speed changed; however, some variables changed more than others, indicating that range of motion and angular velocity for some joints may be more closely related to post-impact ball speed than for other joints.
Systematic Review and Meta-Analysis of the Effectiveness and Safety of Tigecycline for Treatment of Infectious Disease (2010)

The aim of this study was to compare the efficacy and safety of tigecycline, a newly developed glycylcycline antibiotic, with those of empirical antibiotic regimens which have been reported to possess good efficacy for complicated skin and skin structure infections (cSSSIs), complicated intra-abdominal infections (cIAIs), community-acquired pneumonia (CAP), and other infections caused by methicillin-resistant Staphylococcus aureus (MRSA) or vancomycin-resistant Enterococcus (VRE). A meta-analysis of randomized controlled trials (RCTs) identified in PubMed, the Cochrane Library, and Embase was performed. Eight RCTs involving 4,651 patients were included in the meta-analysis. Compared with therapy with empirical antibiotic regimens, tigecycline monotherapy was associated with similar clinical treatment success rates (for the clinically evaluable [CE] population, odds ratio [OR] = 0.92, 95% confidence interval [CI] = 0.76 to 1.12, P = 0.42; for the clinical modified intent-to-treat [c-mITT] population, OR = 0.86, 95% CI = 0.74 to 1.01, P = 0.06) and similar microbiological treatment success rates (for the microbiologically evaluable [ME] population, OR = 0.86, 95% CI = 0.69 to 1.07, P = 0.19). The incidence of adverse events in the tigecycline group was significantly higher than that in the other therapy groups with a statistical margin (for the modified intent-to-treat [mITT] population, OR = 1.33, 95% CI = 1.17 to 1.52, P < 0.0001), especially in the digestive system (mITT population, OR = 2.41, 95% CI = 1.67 to 3.46, P < 0.00001). No difference regarding all-cause mortality and drug-related mortality between tigecycline and the other regimens was found, although numerically higher mortality was found in the tigecycline group. This meta-analysis provides evidence that tigecycline monotherapy may be used as effectively as the comparison therapy for cSSSI, cIAIs, CAP, and infections caused by MRSA/VRE. However, because of the high risk of mortality, AEs, and emergence of resistant isolates, prudence with the clinical use of tigecycline monotherapy in infections is required.
Electrophoresis of Hemoglobin in Single Erythrocytes (1965)

A technique for electrophoretic analysis of the hemoglobin mixtures from single erythrocytes has been developed. Hemoglobin mixtures were separated into their constituents, A(2), A, C, S, and J, at least qualitatively.
T lymphoid differentiation in human bone marrow (2003)

The unique role of the thymus in the development of T cells was established >4 decades ago. To elucidate how uncommitted lymphoid progenitor cells are instructed to migrate from bone marrow to the thymus to undergo T lymphoid differentiation, we generated and analyzed a genome-wide gene expression profile of CD7+ CD10+ human bone marrow T cell lineage precursors (TLPs) by using the serial analysis of gene expression technique. Unexpectedly, the serial analysis of gene expression profile identified a high number of (pre-) T cell receptor antigen (TCR)-related transcripts in bone marrow TLPs. To determine the configuration of the TCRbeta locus in these cells at a quantitative level, we sorted and analyzed bone marrow TLPs from five donors by single-cell PCR. Similar proportions of TLPs harbored TCRbeta germ-line alleles, D-J, or V-DJ gene rearrangements. Thus, bone marrow TLPs are heterogenous with respect to TCRbeta rearrangement status, suggesting an active recombination machinery that is consistent with the expression of RAG1, RAG2, and TdT in this population. As a hallmark of ongoing TCRbeta V-DJ rearrangement, we could amplify broken-ended recombination-signal sequence DNA intermediates from bone marrow TLPs, but not from mature T cells by ligation-mediated PCR. Approximately half of the TCRbeta rearrangements were compatible with the expression of a functional pre-TCR, which is in agreement with surface expression of pre-Talpha on bone marrow TLPs as shown by confocal laser microscopy and flow cytometry. At a frequency <0.5% of mononucleated cells in human bone marrow, this population is rare, yet it exemplifies T lymphoid differentiation in the human already before immigration into the thymus.
DNA damage repair and tolerance: a role in chemotherapeutic drug resistance (2013)

A significant barrier to effective cancer therapy is the development of resistance to the drugs utilised. Standard chemotherapeutic regimens typically contain genotoxic agents, designed to damage DNA of existing tumour cells as well as prevent the synthesis of new DNA during proliferation. DNA damage in normal cells can be repaired efficiently or tolerated to preserve cellular and organ functionality. The mechanisms of DNA repair and tolerance are distinct for different types of lesion, but can be predicted if the mechanism of interaction of the drug with the DNA is known. There is now evidence in solid tumours to suggest that increased repair or tolerance of DNA lesions may contribute to the ability of the cancer cell to survive in high genotoxic stress environments afforded by the therapy. This review will explore the current understanding of drug resistance mechanisms to chemotherapy, but will focus on the new evidence for tolerance and repair, including some new data from the authors' laboratory on the haematological malignancy multiple myeloma. The review will focus particularly on the role of the 'specialised polymerases' which have flexible active sites capable of accommodating DNA lesions, allowing replication past the lesion by translesion synthesis and tolerance of the damage, which ultimately results in a phenotype of drug resistance.
Spectral and temporal control of an actively mode-locked fiber laser (2009)

We report theoretical and experimental investigations on the spectral and temporal control of a mode-locked fiber laser using a chirped fiber Bragg grating and a loss modulator in either a undirectionnal ring cavity or a standing-wave cavity. The fiber laser generates picosecond pulses with a rapid tuning over a large bandwidth. Tuning is achieved by controlling the frequency of the applied modulation waveform. The adjustement of pulse duration between 40 - 500 ps and the rapid tuning from 1513 nm to 1588 nm are described.
Error-Correcting Codes with Byte Error-Detection Capability (1983)

Single error-correcting and double error-detecting codes capable of detecting all single byte errors are important for practical applications. They can be used to enhance the reliability and the data integrity of computer memory systems. Here we present the construction of these codes. The construction techniques are developed from the theory of orthogonal flats in finite Euclidean geometry.