gebhart/patrepeval huggingface dataset:
Phosphoglucomutase 1: complete human and rabbit mRNA sequences and direct mapping of this highly polymorphic marker on human chromosome 1. (1992)

A cDNA clone encoding the mRNA for the highly polymorphic human enzyme phosphoglucomutase 1 (PGM1; EC 5.4.2.2) has been isolated and characterized. This was achieved indirectly by first isolating a rabbit cDNA from an expression library using anti-rabbit PGM antibodies. A comparison of the nucleotide sequences shows that the homologies between human and rabbit PGM1 mRNAs are 92% and 97% for the coding nucleotide sequence and the amino acid sequence, respectively. The derived rabbit amino acid sequence is in complete agreement with the published protein sequence for rabbit muscle PGM. A physical localization of the human PGM1 gene to chromosome 1p31 has been determined by in situ hybridization. Analysis of DNA from a wide variety of vertebrates indicates a high level of PGM1 sequence conservation during evolution.
ChemInform Abstract: NEW ANTISECRETORY AND ANTIULCER ALKOXYTHIOBENZAMIDES. II (1979)

Abstract Nach bekannten Methoden (meist Thionierung des Benzamids mit Phosphorpentasulfid in Dioxan bzw. Pyridin oder Willgerodt‐Kindler‐Reaktion des Benzaldehyds mit Schwefel und dem heterocyclischen Amin) wurden 33 N‐heterocyclische Thiobenzamide wie (I)‐(VI) synthetisiert.
Effects of phase change on reflection in phase-measuring interference microscopy (2004)

We show by analytical and numerical calculations that the phase change on reflection that occurs in interference microscopy is almost independent of the numerical aperture of the objective. The shift of the microscope interferogram response due to the phase change on reflection, however, increases with the numerical aperture. Measurements of the interferogram shift are made with a Linnik interference microscope equipped with various numerical-aperture objectives and are reported and compared with theory.
Flash photolysis of per[oxydi]sulfate ions in aqueous solutions. The sulfate and ozonide radical anions (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFlash photolysis of per[oxydi]sulfate ions in aqueous solutions. The sulfate and ozonide radical anionsLibera Dogliotti and Elie HayonCite this: J. Phys. Chem. 1967, 71, 8, 2511–2516Publication Date (Print):July 1, 1967Publication History Published online1 May 2002Published inissue 1 July 1967https://pubs.acs.org/doi/10.1021/j100867a019https://doi.org/10.1021/j100867a019research-articleACS PublicationsRequest reuse permissionsArticle Views1376Altmetric-Citations297LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Abnormal expression of the ATM and TP53 genes in sporadic breast carcinomas. (2000)

The ataxia telangiectasia gene (ATM) has been implicated as a risk factor in the development of sporadic breast carcinomas. ATM protein expression was analyzed by immunohistochemistry in 17 breast carcinomas with two monoclonal antibodies whose immunohistochemical use was first validated by comparing the immunoreactivity observed in spleen samples from ataxia telangiectasia and trauma patients. In normal breast ducts, ATM showed nuclear expression in the epithelial but not in the myoepithelial cells. In contrast, this nuclear expression was absent or low in the epithelial cancer cells in 10 of 17 (59%) of the tumors studied. Allelic imbalance in the ATM gene was found in three of seven tumors examined. Two of these showed reduced ATM protein expression, but this did not correlate with the presence of ATM mutations in the tumor DNA detected by restriction endonuclease fingerprinting screening. These results suggest that the reduced ATM protein expression could be attributable, in certain tumors, to deletions or rearrangements within or close to the ATM gene. Positive p53 immunostaining was found in 10 tumors, with TP53 mutations detected in 8. Three tumors had both low ATM expression and mutated TP53. Our results indicate that in the majority (15 of 17) of the sporadic breast carcinomas examined, not only is the functionality of the ATM-p53-mediated DNA damage response compromised, but also other signaling pathways activated by these two multifunctional proteins are likely to be impaired, which could be a contributing factor to tumor development and progression.
Driver Fatigue Detection based on Eye State Analysis (2008)

In this paper, we present an effective vision-based driver fatigue detection method. Firstly, the interframe difference approach binding color information is used to detect face. If exists, the face area is segmented from the image based on a mixed skin tone model. Then we simulate the process of crystallization to obtain the location of eyes within face area. Later, eye area, average height of the pupil and width to height ratio are used to analyze the eye's status. Finally, the driver fatigue is confirmed by analyzing the changes of eye's states. The experimental results show validity of our proposed method.
Simultaneous long offset (SLO) towed streamer seismic acquisition (2013)

An innovative 3D towed streamer project in offshore Gabon used a dual-vessel continuous long offset streamer configuration to acquire 0-12 km offsets with ten dual- sensor streamers. Streamer control for the 6 km streamers was robust and avoided operational complications or logistical penalties. Simultaneous shooting maximized inline shot density for long record lengths, thus capturing unaliased deep target reflections from rugose base-salt and sub-salt horizons. Survey design benefited from prior 2D survey experiences with a variety of broadband source and streamer technologies, and the use of 2D streamers as long as 12 km. 3D illumination modelling further suggested that offsets as long as 16 km could be expected to yield useful base-salt and sub-salt reflections.Wavefield separation processing yielded full receiver- side deghosting onboard, followed by an inversion-based separation of simultaneous shots onshore. The ultra-long 12 km offsets combined with strong amplitudes of deghosted low frequencies have yielded encouraging sub- salt and pre-salt imaging.
Bidding Strategy of Virtual Power Plant for Participating in Energy and Spinning Reserve Markets—Part I: Problem Formulation (2010)

This paper addresses the bidding problem faced by a virtual power plant (VPP) in a joint market of energy and spinning reserve service. The proposed bidding strategy is a non-equilibrium model based on the deterministic price-based unit commitment (PBUC) which takes the supply-demand balancing constraint and security constraints of VPP itself into account. The presented model creates a single operating profile from a composite of the parameters characterizing each distributed energy resources (DER), which is a component of VPP, and incorporates network constraints into its description of the capabilities of the portfolio. The presented model is a nonlinear mixed-integer programming with inter-temporal constraints and solved by genetic algorithm (GA).
Synthesis of Ferrocenyl Alkenes, Dienes, and Enynes via Samarium Diiodide Promoted Tandem Addition and Dehydration of Ferrocenyl Carbonyls with Halides (2001)

A practical method for preparation of ferrocenyl alkenes, dienes, and enynes from ferrocenyl carbonyls was explored. A one-pot operation using samarium diiodide to promote the condensation reactions of ferrocenecarboxaldehyde, acetylferrocene, benzoylferrocene, and butanoylferrocene with benzyl bromides, allyl bromide, propargyl bromide, and 1-bromo-3-chlorobutane gave the olefinic products in very high yields. The condensation reactions were also achieved by using catalytic amounts of SmI(2) with magnesium to regenerate the divalent samarium reducing agent.
O-Selectivity and Utility of Phosphorylation Mediated by Phosphite Triester Intermediates in the N-Unprotected Phosphoramidite Method (2004)

Previously, O-selective phosphorylation on polymer supports in the N-unprotected phosphoramidite method could not be carried out because the amino groups of dA and dC have high reactivity toward tervalent phosphorus(III)-type phosphitylating reagents. In this paper, we developed a new coupling strategy named the "activated phosphite method" in which the phosphitylation is mediated by phosphite triester intermediates 1. Application of 1-hydroxybenzotriazole as the promoter to the solid-phase synthesis resulted in excellent O-selectivity of more than 99.7%. This O-selectivity was explained by the frontier molecular orbital interactions between the reactive intermediates and the nucleophiles such as the amino or hydroxyl groups of nucleosides. Furthermore, longer oligonucleotides were synthesized not only by a manual operation but also by a DNA synthesizer. The utility of our new method was demonstrated by the successful synthesis of a base-labile modified oligodeoxyribonucleotide having 4-N-acetyldeoxycytidine residues. Finally, DNA 20-mers containing dA or dC could be synthesized in good yields by use of a combined reagent of 6-trifluoromethyl-1-hydroxybenzotriazole and benzimidazolium triflate.
The healing of hamster skin ulcers treated with <i>n</i>‐butyl‐2‐cyanoacrylate (Histoacryl blue) (1984)

This study was undertaken to examine the effectiveness of a commercially available n-butyl-2-cyanoacrylate (Histoacryl blue+) as a local treatment for cutaneous ulcers. Two ulcers, approximately 0.5 cm2 each, were made on the backs of 30 hamsters. The right side was covered with a thin film of tissue adhesive, while the left side was left untreated as a control. Animals were sacrificed at various times post-operatively, the tissue excised, processed, and examined with the light microscope. Results showed that cyanoacrylate decreased the inflammatory exudate early in the experiment, and epithelial migration occurred slightly earlier in experimental tissue. Scab formation was absent in experimental sites until the layer of adhesive was lost. After 2 days, healing was comparable in both experimental and control, and the sites were indistinguishable histologically at day 5.
Nanoindentation testing of SU-8 photoresist mechanical properties (2008)

In this work, quasi-static and dynamic tests for the mechanical properties of SU-8 using a nanomechanical triboindenter were performed. Load-displacement data for the indenter as it is driven into ...
How much peptide sequence information is contained in ion trap tandem mass spectra? (2008)

Matching peptide tandem mass spectra to their cognate amino acid sequences in databases is a key step in proteomics. It is usually performed by assigning a score to a spectrum-sequence combination. De novo sequencing or partial de novo sequencing is useful for organisms without sequenced genome or for peptides with unexpected modifications. Here we use a very large, high accuracy proteomic dataset to investigate how much peptide sequence information is present in tandem mass spectra generated in a linear ion trap (LTQ). More than 400,000 identified tandem mass spectra from a single human cancer cell line project were assigned to 26,896 distinct peptide sequences. The average absolute fragment mass accuracy is 0.102 Da. There are on average about four complementary b- and y-ions; both series are equally represented but y ions are 2- to 3-fold more intense up to mass 1000. Half of all spectra contain uninterrupted b- or y-ion series of at least six amino acids and combining b- and y-ion information yields on average seven amino acid sequences. These sequences are almost always unique in the human proteome, even without using any precursor or peptide sequence tag information. Thus, optimal de novo sequencing algorithms should be able to obtain substantial sequence information in at least half of all cases.
A Wearable Fabric Computer by Planar-Fashionable Circuit Board Technique (2009)

A method to fabricate circuits on the cloth, planar fashionable circuit board (P-FCB), is proposed. And its applications such as fabric passive elements, user I/O interface, and the fabric package are introduced. The electrical and the mechanical characteristic analysis of P-FCB and the system integration methodology establishment improve the system performance and productivity. A complete wearable system is implemented by P-FCB technology for the continuous sweat monitoring and the RFID tag antenna applications.
Skeletal Muscle Reprogramming by Activation of Calcineurin Improves Insulin Action on Metabolic Pathways (2003)

The protein phosphatase calcineurin is a signaling intermediate that induces the transformation of fast-twitch skeletal muscle fibers to a slow-twitch phenotype. This reprogramming of the skeletal muscle gene expression profile may have therapeutic applications for metabolic disease. Insulin-stimulated glucose uptake in skeletal muscle is both impaired in individuals with type II diabetes mellitus and positively correlated with the percentage of slow- versus fast-twitch muscle fibers. Using transgenic mice expressing activated calcineurin in skeletal muscle, we report that skeletal muscle reprogramming by calcineurin activation leads to improved insulin-stimulated 2-deoxyglucose uptake in extensor digitorum longus (EDL) muscles compared with wild-type mice, concomitant with increased protein expression of the insulin receptor, Akt, glucose transporter 4, and peroxisome proliferator-activated receptor-gamma co-activator 1. Transgenic mice exhibited elevated glycogen deposition, enhanced amino acid uptake, and increased fatty acid oxidation in EDL muscle. When fed a high-fat diet, transgenic mice maintained superior rates of insulin-stimulated glucose uptake in EDL muscle and were protected against diet-induced glucose intolerance. These results validate calcineurin as a target for enhancing insulin action in skeletal muscle.
Eloquent silence: developmental functions of Class I histone deacetylases (2008)

Histone deacetylases (HDACs) are essential catalytic components of the transcription silencing machinery and they play important roles in the programming of multicellular development. HDACs are present within multisubunit protein complexes, other components of which govern HDAC target gene specificity by controlling interactions with sequence-specific DNA-binding proteins. Here, I review the different developmental roles of the Sin3, NuRD, CoREST and NCoR/SMRT Class I HDAC complexes. With their distinct subunit composition, these versatile molecular devices function in many different settings, to promote axis specification and tissue patterning, to maintain stem cell pluripotency, facilitate self-renewal, guide lineage commitment and drive cell differentiation.
The role of HLA class II genes in insulin-dependent diabetes mellitus: molecular analysis of 180 Caucasian, multiplex families. (1996)

We report here our analysis of HLA class II alleles in 180 Caucasian nuclear families with at least two children with insulin-dependent diabetes mellitus (IDDM). DRB1, DQA1, DQB1, and DPB1 genotypes were determined with PCR/sequence-specific oligonucleotide probe typing methods. The data allowed unambiguous determination of four-locus haplotypes in all but three of the families. Consistent with other studies, our data indicate an increase in DR3/DR4, DR3/DR3, and DR4/DR4 genotypes in patients compared to controls. In addition, we found an increase in DR1/DR4, DR1/DR3, and DR4/DR8 genotypes. While the frequency of DQB1*0302 on DR4 haplotypes is dramatically increased in DR3/DR4 patients, DR4 haplotypes in DR1/DR4 patients exhibit frequencies of DQB1*0302 and DQB1*0301 more closely resembling those in control populations. The protective effect of DR2 is evident in this data set and is limited to the common DRB1*1501-DQB1*0602 haplotype. Most DR2+ patients carry the less common DR2 haplotype DRB1*1601-DQB1*0502, which is not decreased in patients relative to controls. DPB1 also appears to play a role in disease susceptibility. DPB1*0301 is increased in patients (P < .001) and may contribute to the disease risk of a number of different DR-DQ haplotypes. DPB1*0101, found almost exclusively on DR3 haplotypes in patients, is slightly increased, and maternal transmissions of DRB1*0301-DPB1*0101 haplotypes to affected children occur twice as frequently as do paternal transmissions. Transmissions of DR3 haplotypes carrying other DPB1 alleles occur at approximately equal maternal and paternal frequencies. The complex, multigenic nature of HLA class II-associated IDDM susceptibility is evident from these data.
A Subset of <i>Drosophila</i> Integrator Proteins Is Essential for Efficient U7 snRNA and Spliceosomal snRNA 3′-End Formation (2010)

Proper gene expression relies on a class of ubiquitously expressed, uridine-rich small nuclear RNAs (snRNAs) transcribed by RNA polymerase II (RNAPII). Vertebrate snRNAs are transcribed from a unique promoter, which is required for proper 3'-end formation, and cleavage of the nascent transcript involves the activity of a poorly understood set of proteins called the Integrator complex. To examine 3'-end formation in Drosophila melanogaster, we developed a cell-based reporter that monitors aberrant 3'-end formation of snRNA through the gain in expression of green fluorescent protein (GFP). We used this reporter in Drosophila S2 cells to determine requirements for U7 snRNA 3'-end formation and found that processing was strongly dependent upon nucleotides located within the 3' stem-loop as well as sequences likely to comprise the Drosophila equivalent of the vertebrate 3' box. Substitution of the actin promoter for the snRNA promoter abolished proper 3'-end formation, demonstrating the conserved requirement for an snRNA promoter in Drosophila. We tested the requirement for all Drosophila Integrator subunits and found that Integrators 1, 4, 9, and 11 were essential for 3'-end formation and that Integrators 3 and 10 may be dispensable for processing. Depletion of cleavage and polyadenylation factors or of histone pre-mRNA processing factors did not affect U7 snRNA processing efficiency, demonstrating that the Integrator complex does not share components with the mRNA 3'-end processing machinery. Finally, flies harboring mutations in either Integrator 4 or 7 fail to complete development and accumulate significant levels of misprocessed snRNA in the larval stages.
Microscopy by reconstructed wave-fronts (1949)

Abstract The subject of this paper is a new two-step method of optical imagery. In a first step the object is illuminated with a coherent monochromatic wave, and the diffraction pattern resulting from the interference of the coherent secondary wave issuing from the object with the strong, coherent background is recorded on a photographic plate. If the photographic plate, suitably processed, is replaced in the original position and illuminated with the coherent background alone, an image of the object will appear behind it, in the original position. It is shown that this process reconstructs the coherent secondary wave, together with an equally strong ‘twin wave’ which has the same amplitude, but opposite phase shifts relative to the background. The illuminating wave itself can be used for producing the coherent background. The simplest case is illumination by a point source. In this case the two twin waves are shown to correspond to two ‘twin objects’, one of which is the original, while the other is its mirror image with respect to the illuminating centre. A physical aperture can be used as a point source, or the image of an aperture produced by a condenser system . If this system has aberrations, such as astigmatism or spherical aberration, the twin image will be no longer sharp but will appear blurred, as if viewed through a system with twice the aberrations of the condenser. In either case the correct image of the object can be effectively isolated from its twin, and separately observed. Three-dimensional objects can be reconstructed, as well as two-dimensional. The wave used in the reconstruction need not be the original, it can be, for example, a light-optical imitation of the electron wave with which the diffraction diagram was taken. Thus it becomes possible to extend the idea of Sir Lawrence Bragg’s ‘X -ray microscope’ to arbitrary objects, and use the new method for improvements in electron microscopy. The apparatus will consist of two parts, an electronic device in which a diffraction pattern is taken with electrons diverging from a fine focus, and an optical synthetizer, which imitates the essential data of the electronic device on a much enlarged scale. The theory of the analysis-synthesis cycle is developed, with a discussion of the impurities arising in the reconstruction, and their avoidance. The limitations of the new method are due chiefly to the small intensities which are available in coherent beams, but it appears perfectly feasible to achieve a resolution limit of 1 Å, ultimately perhaps even better.
A survey of proteomic biomarkers for heterotopic ossification in blood serum (2017)

This study is the first reported SRM-MS analysis of serum from individuals with and without heterotopic ossification, and differences in the serum proteomic profile between healthy and diseased subjects were identified. Furthermore, our results indicate that normal wound healing signals can impact the ability to identify biomarkers, and a multi-protein panel assay, including osteocalcin preproprotein, osteomodulin precursor, and collagen alpha-1(v) chain isoform 2 preprotein, may provide a solution for HO detection and monitoring.
Coronary artery characterization based on tissue elasticity imaging - in vivo assessment (2003)

Diagnosis of pathologies like atherosclerotic plaque is important for preventing plaque rupture, which induces acute ischemic syndromes. We investigated a method of diagnosing plaque based on elasticity imaging using IVUS that reflects mechanical properties of the tissue of arteries. The aim of this study is to investigate the potential of the developed measurement systems for characterizing coronary arteries. To this end, we conducted in-vivo tests of performance and demonstrated that different types of plaque can be discriminated, revealing the potential of elasticity imaging for diagnosing types of plaque and preventing plaque rupture.
Accumulation of Fibronectin in Articular Cartilage Explants Cultured with TGFβ1 and Fucoidan (1995)

Fibronectin is a glycoprotein involved in cell matrix interactions. In osteoarthritis, fibronectin levels in the lesion cartilage are elevated up to 20-fold above control levels. In these experiments, explants of disease-free cartilage cultured in the presence of a combination of TGF beta 1 and the sulfated fucopolysaccharide, fucoidan, accumulated fibronectin at levels comparable to those found in osteoarthritic lesions. TGF beta 1 increased fibronectin synthesis, most of which was released to the medium. The addition of fucoidan favored retention of the newly synthesized fibronectin within the matrix. The fibronectin which accumulated as a result of these treatments was similar to the fibronectin in normal and osteoarthritic cartilage with respect to the ED-B+ alternative splice form. No change in the proteoglycan content of the cartilage explants with elevated fibronectin levels was detected.