gebhart/patrepeval huggingface dataset:
Two‐dimensional gel electrophoresis, protein electroblotting and microsequencing: A direct link between proteins and genes (1990)

We have used two-dimensional gel electrophoresis as a general "preparative" method to purify proteins for microsequencing analysis. In the first experiments, proteins derived from a total extract of Nicotiana tabacum leaf tissue were directly blotted from the gel onto poly(4-vinyl-N-methylpyridinium iodide)-coated glass fiber sheets. The major spots were excised and subjected to NH2-terminal sequence analysis, which made it possible to identify five of the eight selected proteins, while two more were recognized by generated internal sequences. In a second set of experiments, proteins of human origin were separated on multiple two-dimensional gels and the Coomassie Brilliant Blue-stained spots were excised from the gels. The combined spots were re-eluted and concentrated in a new gel and blotted on Immobilon. They were fragmented by in situ proteolysis and the generated peptides were separated by reverse phase-high performance liquid chromatography and sequenced. At the average, the internal sequences that were obtained covered 35 residues per protein and allowed unambiguous identification of 13 of the 23 proteins analyzed so far. The sequence information obtained of the unidentified proteins is sufficient for further cloning. These results demonstrate that systematic sequence analysis of the major proteins seen in two-dimensional gels is within the reach of current technologies. This offers a unique opportunity to link information contained in protein databases with known or forthcoming DNA sequence data.
IMS vs. P2P and Web 2.0 - Understanding the Role of the IP Multimedia System (IMS) in Face of a Converging Telco and Internet Service World (2008)

This tutorial will introduce the notion of network convergence and next generation networks by looking at the evolution of telecommunication services and Internet services and the related infrastructure. Special emphasis will be placed on describing the principles and architecture of the IP multimedia subsystem (IMS), a common service platform standard defined by IETF, 3GPP, ETSI TISPAN, and Cablelab, which is based on internet protocols and intelligent network principles. Besides the IMS core operation also the IMS application provisioning principles will be illustrated. It will be shown that IMS is well designed to support seamless presence and community oriented multimedia information and communication services across various networks. However, a critical comparison of IMS with classic VoIP infrastructures, P2P service platforms and the emerging Web 2.0 will be performed. The tutorial ends with an introduction of the Fraunhofer FOKUS Open IMS Playground - a globally known pioneering IMS testbed, and the corresponding open source IMS core system, which has been released in 2006 and since then provides the foundation for many academic and industry NGN/IMS testbeds around the globe.
Phenotypic characterization of endometrial stromal sarcoma of the uterus (2006)

Endometrial stromal sarcoma (ESS) of the uterus is a rare uterine malignancy that has not been characterized in detail. To characterize the phenotype of ESS of the uterus, we extracted RNA from ESS and the stroma of normal endometrium using a tissue microdissection system and compared the expression profiles in the two tissues. After suppression subtractive hybridization and differential screening, we detected the metastasis-associated lung adenocarcinoma transcript 1 (MALAT-1) gene as one of the major genes upregulated in ESS, and a full-length placental cDNA clone (CS0DI066YJ10) as one of the major genes downregulated. The results were confirmed by in situ hybridization in four resected specimens of ESS and 36 biopsy specimens of normal endometrial tissue. All ESS (4/4) and all cases of endometrial stromal cells in the proliferative phase (13/13) were positive for MALAT-1, but samples of normal stroma in the secretory phase and menopausal state included some that were negative or weakly positive for MALAT-1 (5/13 and 3/10, respectively). In contrast, all ESS and 12 of 13 cases of stromal cells in the proliferative phase were negative for the full-length placental cDNA clone but 10 of 13 cases of endometrial stromal cells in the secretory phase were positive for transcripts of the gene (P < 0.05). These results indicated that endometrial stromal cells have different phenotypic characteristics between proliferative and secretory phases and the tumor cells of ESS have the phenotypic character of endometrial stromal cells in the proliferative phase.
Developing a zero‐AOX shrink‐resist process for wool. Part 1: Preliminary results (2001)

The efficiency of the proteolytic enzyme papain in conferring shrink‐resistance to wool tops and woven fabrics has been enhanced by pretreatment of the wool with lipase/sodium monoperoxyphthalate/sodium sulphite. This process may be considered as a zero‐AOX shrink‐proofing treatment. The wool samples treated with this system show excellent shrink‐proofed properties. Infrared spectroscopy and X‐ray photoelectron spectroscopy showed that the Buntë salt together with low concentrations of cystine monoxide and cystine dioxide are formed in the course of the reaction. Studies of the surface of the treated wool using scanning electron microscopy have shown the complete absence of wool scales. A mechanism is proposed for shrink resistance in the lipase/sodium monoperoxyphthalate/papain treatment.
Resolved Motion Rate Control of Manipulators and Human Prostheses (1970)

The kinematics of remote manipulators and human prostheses is analyzed for the purpose of deriving resolved motion rate control. That is, the operator is enabled to call for the desired hand motion directly along axes relevant to the task environment. The approach suggests solutions to problems of coordination, motion under task constraints, and appreciation of forces encountered by the controlled hand.
Catalytic Isomerization of Vegetable Oils: Nickel Catalysts (1946)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic Isomerization of Vegetable Oils: Nickel CatalystsS. B. Radlove, H. M. Teeter, W. H. Bond, J. C. Cowan, and J. P. KassCite this: Ind. Eng. Chem. 1946, 38, 10, 997–1002Publication Date (Print):October 1, 1946Publication History Published online1 May 2002Published inissue 1 October 1946https://pubs.acs.org/doi/10.1021/ie50442a011https://doi.org/10.1021/ie50442a011research-articleACS PublicationsRequest reuse permissionsArticle Views187Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Au/Pt/Ti contacts to <i>p</i>-In0.53Ga0.47As and <i>n</i>-InP layers formed by a single metallization common step and rapid thermal processing (1990)

We have demonstrated the viability of depositing a thick Au bonding pad on top of Pt/Ti contacts on both p-InGaAs and n-InP within a single evaporation prior to heat treatment. This eliminates the usual post-sinter Au plating process. In particular, Au (500 nm)/Pt (60 nm)/Ti (50 nm) common contacts to Zn-doped 5×1018 cm−3 p-In0.53Ga0.47As and S-doped 1×1018 cm−3 n-InP were formed within a single pumpdown electron-gun evaporation and subsequently a single sintering process by means of rapid thermal processing. The lowest resistivity of these ohmic contacts were found to be 0.11 and 0.13 Ω mm (5.5×10−7 and 8×10−6 Ω cm2) for the p and n contacts, respectively. These values were achieved as a result of heating at 450 °C for 30 sec. This heat treatment caused a limited reaction at the Au-Pt and Pt-Ti interfaces, which did not lead to any significant intermixing of the Ti and Au. Thus, no significant indiffusion of the Au thorough the Pt barrier was observed and contact degradation did not occur. The stress of the as-deposited trilayer structure on InP was found to be 3×108 dyne cm2 tensile and increased to about 2×109 dyn cm2 as a result of the rapid thermal processing at 450 °C
Antibiotic treatment for <i>Clostridium difficile</i>-associated diarrhoea in adults (2017)

No firm conclusions can be drawn regarding the efficacy of antibiotic treatment in severe CDI as most studies excluded patients with severe disease. The lack of any 'no treatment' control studies does not allow for any conclusions regarding the need for antibiotic treatment in patients with mild CDI beyond withdrawal of the initiating antibiotic. Nonetheless, moderate quality evidence suggests that vancomycin is superior to metronidazole and fidaxomicin is superior to vancomycin. The differences in effectiveness between these antibiotics were not too large and the advantage of metronidazole is its far lower cost compared to the other two antibiotics. The quality of evidence for teicoplanin is very low. Adequately powered studies are needed to determine if teicoplanin performs as well as the other antibiotics. A trial comparing the two cheapest antibiotics, metronidazole and teicoplanin, would be of interest.
<title>Accommodating geometric and thermodynamic variability for forward-looking infrared sensors</title> (1997)

Our work has focused on deformable template representations of geometric variability in automatic target recognition (ATR). Within this framework we have proposed the generation of conditional mean estimates of pose of ground-based targets remotely sensed via forward-looking IR radar (FLIR) systems. Using the rotation group parameterization of the orientation space and a Bayesian estimation framework, conditional mean estimators are defined on the rotation group with minimum mean squared error (MMSE) performance bounds calculated following. This paper focuses on the accommodation of thermodynamic variation. Our new approach relaxes assumptions of the target's underlying thermodynamic state, expanding thermodynamic state as a scalar field. Estimation within the deformable template setting poses geometric and thermodynamic variation as a joint inference. MMSE pose estimators for geometric variation are derived, demonstrating the 'cost' of accommodating thermodynamic variability. Performance is quantitatively examined, and simulations are presented.
An immunosuppressive agent, FTY720, increases intracellular concentration of calcium ion and induces apoptosis in HL‐60 (1997)

We previously reported that FTY720 is an efficient inducer of apoptosis in lymphocytes and cultured cell lines. In the present study, HL-60 human promyerocytoma cells also induced apoptosis through in vitro treatment with the drug, demonstrating extensive DNA fragmentation 6 hr after incubation. The major target of FTY720 was the common signalling pathway of apoptosis, since a rapid (< 1 min) increase in the intracellular Ca2+ concentration ([Ca2+]i) was found in the cells treated with the drug. Calcium chelation in the culture medium with EGTA did not affect the [Ca2+]i mobilization. A phospholipase C inhibitor, U73122, inhibited the increase in [Ca2+]i as well as the fragmentation of the nuclear DNA, whereas U73343, a non-effective analogue of U73122, had little effect. These results suggest that FTY720-induced apoptosis is mediated through an activation of phospholipase C and the subsequent release of Ca2+ from intracellular calcium pools. In addition, the treatment of HL-60 with pertussis toxin (PTX) did not inhibit Ca2+ mobilization or apoptosis, suggesting that the activation of phospholipase C is independent of PTX-sensitive G-proteins.
The Amyloid Precursor Protein-binding Protein APP-BP1 Drives the Cell Cycle through the S-M Checkpoint and Causes Apoptosis in Neurons (2000)

APP-BP1 binds to the amyloid precursor protein (APP) carboxyl-terminal domain. Recent work suggests that APP-BP1 participates in a novel ubiquitinylation-related pathway involving the ubiquitin-like molecule NEDD8. We show here that, in vivo in mammalian cells, APP-BP1 interacts with hUba3, its presumptive partner in the NEDD8 activation pathway, and that the APP-BP1 binding site for hUba3 is within amino acids 443-479. We also provide evidence that the human APP-BP1 molecule can rescue the ts41 mutation in Chinese hamster cells. This mutation previously has been shown to lead to successive S phases of the cell cycle without intervening G(2), M, and G(1), suggesting that the product of this gene negatively regulates entry into the S phase and positively regulates entry into mitosis. We show that expression of APP-BP1 in ts41 cells drives the cell cycle through the S-M checkpoint and that this function requires both hUba3 and hUbc12. Overexpression of APP-BP1 in primary neurons causes apoptosis via the same pathway. A specific caspase-6 inhibitor blocks this apoptosis. These findings are discussed in the context of abnormalities in the cell cycle that have been observed in Alzheimer's disease.
iPSC-Derived Neural Stem Cells Act via Kinase Inhibition to Exert Neuroprotective Effects in Spinal Muscular Atrophy with Respiratory Distress Type 1 (2014)

Spinal muscular atrophy with respiratory distress type 1 (SMARD1) is a motor neuron disease caused by mutations in the IGHMBP2 gene, without a cure. Here, we demonstrate that neural stem cells (NSCs) from human-induced pluripotent stem cells (iPSCs) have therapeutic potential in the context of SMARD1. We show that upon transplantation NSCs can appropriately engraft and differentiate in the spinal cord of SMARD1 animals, ameliorating their phenotype, by protecting their endogenous motor neurons. To evaluate the effect of NSCs in the context of human disease, we generated human SMARD1-iPSCs motor neurons that had a significantly reduced survival and axon length. Notably, the coculture with NSCs ameliorate these disease features, an effect attributable to the production of neurotrophic factors and their dual inhibition of GSK-3 and HGK kinases. Our data support the role of iPSC as SMARD1 disease model and their translational potential for therapies in motor neuron disorders.
Fast Dissolving Sublingual Films of Ondansetron Hydrochloride: Effect of Additives on in vitro Drug Release and Mucosal Permeation (2010)

Ondansetron hydrochloride, a 5 HT3 antagonist is a powerful antiemetic drug which has oral bioavailability of 60% due to hepatic first pass metabolism and has a short half-life of 5 h. To overcome the above draw back, the present study was carried out to formulate and evaluate fast dissolving films of ondansetron hydrochloride for sublingual administration. The films were prepared from polymers such as polyvinylalcohol, polyvinyl pyrrolidone, Carbopol 934P in different ratios by solvent casting method. Propylene glycol or PEG 400 as plasticizers and mannitol or sodium saccharin as sweeteners were also included. The IR spectral studies showed no interaction between drug and polymer or with other additives. Satisfactory results were obtained when subjected to physico-chemical tests such as uniformity of weight, thickness, surface pH, folding endurance, uniformity of drug content, swelling index, bioadhesive strength, and tensile strength. Films were also subjected to in vitro drug release studies by using USP dissolution apparatus. Ex vivo drug permeation studies were carried out using porcine membrane model. In vitro release studies indicated 81-96% release within 7 min and 66-80% within 7 min during ex vivo studies. Drug permeation of 66-77% was observed through porcine mucosa within 40 min. Higher percentage of drug release was observed from films containing the sweeteners. The stability studies conducted for a period of 8 weeks showed no appreciable change in drug content, surface pH, and in vitro drug release.
Cyclic GMP-Dependent Protein Kinases and the Cardiovascular System (2003)

Signaling cascades initiated by nitric oxide (NO) and natriuretic peptides (NPs) play an important role in the maintenance of cardiovascular homeostasis. It is currently accepted that many effects of these endogenous signaling molecules are mediated via stimulation of guanylyl cyclases and intracellular production of the second messenger cGMP. Indeed, cGMP-elevating drugs like glyceryl trinitrate have been used for more than 100 years to treat cardiovascular diseases. However, the molecular mechanisms of NO/NP signaling downstream of cGMP are not completely understood. Recent in vitro and in vivo evidence identifies cGMP-dependent protein kinases (cGKs) as major mediators of cGMP signaling in the cardiovascular system. In particular, the analysis of conventional and conditional knockout mice indicates that cGKs are critically involved in regulating vascular remodeling and thrombosis. Thus, cGKs may represent novel drug targets for the treatment of human cardiovascular disorders.
The Impact of Lactobacillus plantarum WCFS1 Teichoic Acid D-Alanylation on the Generation of Effector and Regulatory T-cells in Healthy Mice (2013)

To date it remains unclear how probiotics affect the immune system. Bacterial envelope components may play an essential role, as these are the first to establish bacterial-host cell interactions. Teichoic acids (TAs), and especially lipoteichoic acids, are the most pro-inflammatory components of the gram-positive bacterial envelope. This effect is dependent on D-alanyl substitution of the TA backbone and interactions with TLR2 on host cells. Although the pro-inflammatory properties of TAs have been established in vitro, it remains unclear how TAs affect immunomodulation in vivo. In this study, we investigated the role of TA D-alanylation on L. plantarum-induced intestinal and systemic immunomodulation in vivo. For this, we compared the effect of L. plantarum WCFS1 and its TA D-Alanylation negative derivative (dltX-D) on the distribution of dendritic cell and T cell populations and responses in healthy mice. We demonstrated that the majority of the L. plantarum-induced in vivo immunomodulatory effects were dependent on D-alanylation (D-Ala), as some L. plantarum WCFS1-induced immune changes were not observed in the dltX-D-treated group and some were only observed after treatment with dltX-D. Strikingly, not only pro-inflammatory immune responses were abolished in the absence of D-Ala substitution, but also anti-inflammatory responses, such as the L. plantarum-induced generation of regulatory T cells in the spleen. With this study we provide insight in host-microbe interactions, by demonstrating the involvement of D-alanylation of TAs on the bacterial membrane in intestinal and systemic immunomodulation in healthy mice.
Protein stability and molecular adaptation to extreme conditons (1991)

Proteins, due to the delicate balance of stabilizing and destabilizing interactions, are only marginally stable. Adaptation to extreme environments tends to shift the 'mesophilic' characteristics of proteins to the respective extremes of temperature, hydrostatic pressure, pH and salinity, such that, under the mutual physiological conditions, the molecular properties are similar regarding overall topology, flexibility and solvation. Enhanced intrinsic stability requires only minute local structural changes so that general strategies of stabilization cannot be established. Apart from mutative changes of amino-acid sequences, extrinsic factors (or cellular components) may be involved in 'extremophilic adaptation'. The molecular basis of acidophilic, alkalophilic and barophilic adaptation is still obscure. Mechanisms of enhanced thermal stability involve improved packing density, as well as specific local interactions. In halophiles, water and salt binding of the intrinsically stable protein inventory is accomplished by favoring acidic over basic amino acid residues and decreased hydrophobicity. General limits of viability are: (a) the susceptibility of the covalent structure of the polypeptide chain toward hydrolysis or hydrothermal degradation; (b) the competition of extreme solvent parameters with the weak electrostatic and hydrophobic interactions involved in protein stabilization; (c) perturbations of the folding and assembly of proteins; and (d) 'dislocation' of biochemical pathways due to effects of extreme conditions on the intricate network of metabolic reactions.
Robust Training Sequence Design for Correlated MIMO Channel Estimation (2013)

We study how to design a worst-case robust training sequence for multiple-input multiple-output (MIMO) channel estimation. We consider mean-squared error of channel estimates as the figure of merit which is a function of second-order statistics of the MIMO channel, i.e., channel covariance matrix, in order to optimize training sequences under a total power constraint. In practical applications, the channel covariance matrix is not known perfectly. Thus the main aspect of our design is to improve robustness of the training sequences against possible uncertainties in the available channel covariance matrix. Using a deterministic uncertainty model, we formulate a robust training sequence design as a minimax optimization problem where we take such imperfections into account. We investigate the robust design problem assuming the general case of an arbitrarily correlated MIMO channel and a non-empty compact convex uncertainty set. We prove that such a problem admits a globally optimal solution by exploiting the convex-concave structure of the objective function, and propose numerical algorithms to address the robust training design problem. We proceed the analysis by considering multiple-input single-output (MISO) channels and Kronecker structured MIMO channels along with unitarily-invariant uncertainty sets. For these scenarios, we show that the problem is diagonalized by the eigenvectors of the nominal covariance matrices so that the robust design is significantly simplified from a complex matrix-variable problem to a real vector-variable power allocation problem. For the MISO channel, we provide closed-form solutions for the robust training sequences with the uncertainty sets defined by the spectral norm and nuclear norm.
Production of 13C-Labeled Docosahexaenoic Acid by a Thraustochytrid (2000)

A 13C-labeled docosahexaenoic acid (DHA) highly enriched with 13C atoms was prepared using the DHA-producing Thraustochytrid, Schizochytrium sp. strain N1-27. Following 115h-cultivation with sodium acetate-1, 2-13C2 as the sole labeled carbon source, 2.53g/L total lipids were obtained from the cells. The 13C-labeled DHA ethyl ester was prepared from total lipids and fractionated to almost 100% purity with 53% recovery on HPLC. The 13C atom ratio was estimated at more than 97% from GC-MS. The productivity of labeled DHA was 0.29g/L. Other 13C-labeled fatty acids such as docosapentaenoic acid (DPA), eicosapentaenoic acid, and palmitic acid, were obtained as by-products. The present Thraustochytrid is thus shown useful for producing labeled DHA and DPA.