gebhart/patrepeval huggingface dataset:
Affinity chromatography of the anterior pituitary D2-dopamine receptor (1986)

The D2-dopamine receptor from bovine anterior pituitary has been solubilized with digitonin and purified approximately 1000-fold by affinity chromatography on a new affinity support. This support consists of a (carboxymethylene)oximino derivative of the D2-selective antagonist spiperone (CMOS) covalently attached to Sepharose 4B through a long side chain. The interaction of the solubilized receptor activity with the affinity gel was biospecific. Dopaminergic drugs blocked adsorption of solubilized receptor activity to the CMOS-Sepharose with the appropriate D2-dopaminergic potency and stereoselectivity. For agonists, (-)-N-n-propylnorapomorphine greater than 2-amino-6,7-dihydroxytetrahydronaphthalene approximately equal to apomorphine greater than dopamine, whereas for antagonists (+)-butaclamol much greater than (-)-butaclamol. The same D2-dopaminergic specificity was observed for elution of receptor activity from the gel. To observe eluted receptor binding activity, reconstitution of the eluted material into phospholipid vesicles was necessary. Typically, 70-80% of the solubilized receptor was adsorbed by CMOS-Sepharose, and 40-50% of the adsorbed activity could be recovered after reconstitution of the eluted material. The overall recovery of D2-receptor activity from bovine anterior pituitary membranes was 12-15% with specific binding activity of approximately 150 pmol/mg. The reconstituted affinity-purified receptor bound ligands with the expected D2-dopaminergic specificity, stereoselectivity, and rank order of potency.
Synthesis of [3″‐<sup>3</sup>H]taxol and [13‐<sup>3</sup>H]taxol<sup>1</sup> (1993)

Abstract 7‐Trietylsilylbaccatin III ( 11 ) was coupled with cis‐1‐benzoyl‐3‐triethylsiloxy‐4‐(3′‐bromophenyl)azetidin‐2‐one. Hydrolysis of the silyl groups gave 3″‐bromotaxol which was reduced with tritium gas to give[3″‐ 3 H]taxol with specific activity of 19.3 Ci mmol. Reduction of 7‐triethylsilyl‐13‐oxobaccatin III with [ 3 H]borane‐tetrahydrofuran complex gave 7‐triethylsilyl[13‐ 3 H]baccatin III ( 17 ). Coupling of 17 with cis‐3‐triethylsiloxy‐4‐phenyl‐(3R,4S)‐azetidin‐2‐one and hydrolysis gave [13‐ 3 H]taxol with specific activity of 1.66 Ci/mmol.
Enzymatic hydrolysis of cellulose and various pretreated wood fractions (1982)

Three strains of Trichoderma-T. reesei C30, T. reesei QM9414, and Trichoderma species E-58-were used to study the enzymatic hydrolysis of pretreated wood substrates. ach of the culture filtrates was incubated with a variety of commercially prepared cellulose substrates and pretreated wood substrates. Solka floc was the most easily degraded commercial cellulose. The enzyme accessibility of steam-exploded samples which had been alkali extracted and then stored wet decreased with the duration of the steam treatment. Air drying reduced the extent of hydrolysis of all the samples but had a greater effect on the samples which had previously shown the greatest hydrolysis. Mild pulping using 2% chlorite increased the enzymatic hydrolysis of all the samples. Steam explosion was shown to be an excellent pretreatment. The results indicate that the distribution of the lignin as well as the surface area of the cellulosic substrate are important features in enzymatic hydrolysis.
A novel B-cell lineage-specific transcription factor present at early but not late stages of differentiation. (1990)

A novel B-cell-specific transcription factor, BSAP, was identified as a mammalian homolog of the sea urchin protein TSAP, which interacts with the promoters of four tissue-specific late histone H2A-2 and H2B-2 genes. As shown by mobility-shift, methylation interference, and mutational analyses, the mammalian protein BSAP recognizes all four sea urchin binding sites in a manner indistinguishable from TSAP; however, the two proteins differ in molecular weight. BSAP is exclusively restricted to the B-cell lineage of lymphoid differentiation. Its expression appears to be activated during pro-B-cell development, is abundant at the pre-B- and mature B-cell stages, but is absent in terminally differentiated plasma cells. Moreover, BSAP is clearly a B-cell-specific transcription factor, as a wild-type but not a mutant TSAP-binding site of the sea urchin functions only in transfected B cells as an upstream promoter element. Competition experiments did not reveal any high-affinity binding site for BSAP in known regulatory regions of immunoglobulin and class II major histocompatibility (MHC) genes, suggesting that BSAP is a regulator of a different set of B-lymphoid-specific genes.
Kinetic Studies of Ferrous Ion Oxidation with Crystalline Human Ferroxidase (Ceruloplasmin) (1966)

Abstract The kinetics of the catalysis of Fe(II) oxidation by crystalline human ferroxidase (ceruloplasmin) has been studied under various conditions at 30°. Two Km values with respect to Fe(II), Km1 = 0.6 µm and Km2 = 50 µm, were obtained at pH 6.5, at which ferroxidase gave the maximum activity of 550 Fe(II) per ferroxidase per min in an acetate buffer. Competition between the two substrates, p-phenylenediamine and Fe(II), was observed. The stoichiometry of Fe(II), ferroxidase, and oxygen, determined from the experimental data, was 4:1:1.
Demands grow but rewards do not (1998)

Another pay round, another kick in the teeth for nurses. I am sick and tired of this perennial debate about how much nurses are worth and the endless comparison between other public service professions like teaching and the police. We are skilled carers and we are also expected to be administrators, educators, mentors, researchers and towers of strength to all and sundry.
A Retained Bullet in Pericardial Sac: Penetrating Gunshot Injury of the Heart (2016)

Penetrating cardiac trauma is rarely seen but when present there is a short time lag to keep the patients alive. Cardiac gunshot injuries are exceptional and it occurs mostly during interpersonal disagreements casualties or a mistakenly fired gun nowadays. Here we present a case of cardiac gunshot injury from the war of Kobani, Syria. The patient was mistakenly diagnosed to have a sole bullet in the left shoulder while he had a penetrating cardiac trauma with a bullet in the heart and pericardial effusion possibly giving rise to pericardial tamponade. Luckily the cardiac gunshot injury was noticed one day later and the patient was referred to a tertiary hospital. Intrapericardial bullet was conservatively followed up. The patient was discharged one week later after resection of the bullet in the shoulder.
SQUIDs: THEN AND NOW (2010)

Following Brian Josephson's prediction in 1962, Anderson and Rowell observed Josephson tunneling in 1963. The following year, Jaklevic, Lambe, Silver and Mercereau demonstrated quantum interference in a superconducting ring containing two Josephson tunnel junctions. Subsequently, the first practical devices emerged, including the point-contact dc and rf SQUIDs (Superconducting QUantum Interference Devices) of Zimmerman and Silver and Clarke's SLUG (Superconducting Low-inductance Undulatory Galvanometer) — a blob of solder frozen around a length of niobium wire. The return to the tunnel junction as the Josephson element was heralded by the cylindrical SQUID in 1976. The square washer dc SQUID developed by Ketchen and Jaycox in 1982 remains the workhorse design for most applications. Theories for the dc and rf SQUIDs were worked out in the 1970s. Today, SQUIDs (mostly dc) are used in a variety of configurations — for example, as magnetometers, gradiometers, cryogenic current comporators, low-frequency and microwave amplifiers, and susceptometers — in applications including magnetoencephalography, magnetocardiography, geophysics, nondestructive evaluation, precision gyroscopes, standards, cosmology, nuclear magnetic resonance, reading out superconducting quantum bits, and a myriad of one-of-a-kind experiments in basic science. Experiments are described to search for galaxy clusters, hunt for the axion, and perform magnetic resonance imaging in microtesla fields.
NEW YORK ACADEMY OF MEDICINE, SECTION OF OPHTHALMOLOGY (1955)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Ophthalmology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Ophthalmology journal
Quantitative determination of 17 beta-estradiol and progesterone in cellular fractions of term placentae of normal and hypertensive patients. (1992)

During pregnancy the placenta is both the source and target for the actions of steroid hormones. In fact, an intact feto-placental unit is capable of elaborating increasing levels of a wide variety of steroid hormones and many other substances. The falling levels of steroid hormones, like estrogens and progesterone in serum and urine have been reported during pregnancy induced hypertension and/or preeclampsia. The purpose of this study was to determine the concentration of 17 beta-estradiol and progesterone in cytosol, nucleic fraction and particulate fraction of term placentae of normal and hypertensive patients by radioimmunoassay. Data from this study reveal the concentrations of 17 beta-estradiol (E2) and progesterone (P) in various cellular fractions from six placentae of normal patients (NP) and five placentae of hypertensive patients (HP). In HP overall concentrations of E2 (75.8 ng/gm) and P(46.5 micrograms/gm) is greater than those in NP, 32.6 ng/gm and 25.0 micrograms/gm respectively. The concentration of E2 in nuclear fraction of NP and HP remains unchanged while P concentration of nuclear fraction of HP (25.4 micrograms/gm) is much greater than those of NP (8.8 micrograms/gm). Moreover, the E2 concentration (55.5 ng/gm) in cytosol of HP is much greater than those of NP (12.3 ng/gm). Therefore, this study indicates that the abnormal concentrations of E2 and P of placentae are associated with pregnancy induced hypertension.
Differential displacement of the human soleus and medial gastrocnemius aponeuroses during isometric plantar flexor contractions in vivo (2004)

The human triceps surae muscle-tendon complex is a unique structure with three separate muscle compartments that merge via their aponeuroses into the Achilles tendon. The mechanical function and properties of these structures during muscular contraction are not well understood. The purpose of the study was to investigate the extent to which differential displacement occurs between the aponeuroses of the medial gastrocnemius (MG) and soleus (Sol) muscles during plantar flexion. Eight subjects (mean +/- SD; age 30 +/- 7 yr, body mass 76.8 +/- 5.5 kg, height 1.83 +/- 0.06 m) performed maximal isometric ramp contractions with the plantar flexor muscles. The experiment was performed in two positions: position 1, in which the knee joint was maximally extended, and position 2, in which the knee joint was maximally flexed (125 degrees ). Plantarflexion moment was assessed with a strain gauge load cell, and the corresponding displacement of the MG and Sol aponeuroses was measured by ultrasonography. Differential shear displacement of the aponeurosis was quantified by subtracting displacement of Sol from that of MG. Maximal plantar flexion moment was 36% greater in position 1 than in position 2 (132 +/- 20 vs. 97 +/- 11 N.m). In position 1, the displacement of the MG aponeurosis at maximal force exceeded that of the Sol (12.6 +/- 1.7 vs. 8.9 +/- 1.5 mm), whereas in position 2 displacement of the Sol was greater than displacement of the MG (9.6 +/- 1.0 vs. 7.9 +/- 1.2 mm). The amount and "direction" of shear between the aponeuroses differed significantly between the two positions across the entire range of contraction, indicating that the Achilles tendon may be exposed to intratendinous shear and stress gradients during human locomotion.
Scaleable shadow stack for a configurable DSP concept (2004)

SoC (System-on-Chip) applications map complex system functions on a single die. The increasing importance of flexibility in SoC applications leads to raising portion implemented in firmware. Therefore, the demand on computational power of the embedded processors in the application is increasing. The newest silicon technologies (e.g. 0.13 /spl mu/m and lower) help to increase the reachable frequency, but the demand cannot be sufficiently satisfied. One approach to increase the processor frequency is the introduction of pipelining. To guarantee data consistency in deep pipelined processors different methods have been developed. Additional complexity is introduced by the occurrence of interrupts. This paper describes a concept to enable data consistency between the instructions of different pipeline stages in pipelined DSP kernels during interrupt service routines, without the interaction of the DSP itself and with no restrictions concerning the nesting level of the interrupts. The scaleable shadow stack is part of a development project for a configurable DSP concept.
The combined action of hyaluronic acid and fibronectin stimulates neutrophil migration. (1985)

Previous investigations have demonstrated that the chemotactic response of polymorphonuclear leukocytes (PMN) was stimulated by hyaluronic acid (HA) when serum was present. The aim of the present investigation was to identify the serum factor necessary for the stimulation of PMN chemotaxis by HA. By means of gel filtration, the m.w. of the serum component was shown to be greater than 350,000. Immunoprecipitation of serum with anti-fibronectin, but not with anti-IgM and anti-alpha 2-macroglobulin, inhibited the stimulation of PMN chemotaxis by HA. Preincubation of PMN with HA (10 to 500 micrograms/L) and isolated fibronectin (0.1 to 100 mg/L) significantly stimulated the chemotactic response of PMN. Also, random migration of PMN was significantly increased by preincubation of the cells with HA (10 to 500 micrograms/L) and isolated fibronectin (50 to 200 mg/L). Additionally, PMN preincubated with HA (10 to 50 micrograms/L) and with fibronectin (10 to 50 mg/L) added afterwards, and PMN preincubated with fibronectin (10 mg/L) and with HA (5 to 10 micrograms/L) added after the preincubation, showed a significant stimulation of the chemotactic response. PMN preincubated with serum and chondroitin sulfate, or with fibrinogen and HA, demonstrated no stimulation of the chemotactic response. The present investigation suggests that the combined action of HA and fibronectin, which probably takes place at the cellular membrane, is a major mechanism in the HA-mediated stimulation of PMN migration.
A Study on tissue oxygen metabolism using gastric intramucosal pH (gastric pHi) in critically ill patients. (1995)

目的:種々の侵襲に対する過剰な生体反応が組織酸素代謝の失調を来し,細胞障害に続いてさまざまな臓器に障害を引き起こすことが知られている。そこでわれわれは胃粘膜内pH (gastric intramucosal pH,以下gastric pHiと略す)を測定,既存の指標と比較し,重症患者における組織酸素代謝について検討を加え,その失調に対する対策についても若干の検討を加えた。対象と方法:対象は1992年9月より1994年9月までに当救急部ICUに入室した症例の38例である。gastric pHiの他に,酸素供給量係数(以下DOIと略す),酸素消費量係数,血中lactate濃度,動脈血中ケトン体比,osmolality gap, cellular injury score(以下CISと略す)を測定し比較検討した。結果と考察:gastric pHiはlactate, CISと有意の負の相関を示し,組織での酸素の需給バランスがくずれoxygen debtが存在した場合lactate上昇とともにpHiも低下し,続いて細胞障害が発生している可能性が示唆された。一方gastric pHiはDOIが高値を示す領域では有意の負の相関を示した。その理由として以下の二点が考えられた。すなわち第一点としてhyperdynamic stateであるほど細胞のoxygen demandもより増大しているため,それを満たすだけのoxygen uptakeがhumoral mediatorなどのために不可能となっているのではないかということと,第二点として既に不可逆的な細胞障害を生じているためではないかということである。以上より組織酸素代謝の状態を評価するためにはDOIの測定のみならず,組織でのoxygen debtの有無とその結果としての細胞障害の有無を監視することが重要である。gastric pHiは細胞でのoxygen debtを表す指標として有用で,経時的に測定することで組織酸素代謝の変化を早期に発見することが可能となることが示唆された。また組織酸素代謝失調の治療にあたっては,治療効果を判定する指標および最終目標としてgastric pHiは適切であると考えられた。
Novel Fluorometric Assay for Hydroxyl Radical Scavenging Capacity (HOSC) Estimation (2006)

A novel fluorometric method was developed and validated for hydroxyl radical scavenging capacity (HOSC) estimation using fluorescein as the probe. A constant flux of pure hydroxyl radical is generated under physiological pH using a Fenton-like Fe3+/H2O2 reaction. The generation of pure hydroxyl radicals under the experimental conditions was evaluated and confirmed using electron spin resonance with DMPO spin-trapping measurements. The hydroxyl radical scavenging capacity of a selected antioxidant sample is quantified by measuring the area under the fluorescence decay curve with or without the presence of the antioxidant and expressed as Trolox equivalents per unit of the antioxidant. The assay may be performed using a plate reader with a fluorescence detector for high-throughput measurements. The assay was validated for linearity, precision, accuracy, reproducibility, and its correlation with a popular peroxyl radical scavenging capacity assay using selected pure antioxidant compounds and botanical extracts. This method may provide researchers in the food, nutrition, and medical fields an easy to use protocol to evaluate free radical scavenging capacity of pure antioxidants and natural extracts in vitro against the very reactive hydroxyl radical, which may be linked to numerous degenerative diseases and conditions.
SmartPrivacy for the Smart Grid: embedding privacy into the design of electricity conservation (2010)

The 2003 blackout in the northern and eastern U.S. and Canada which caused a $6 billion loss in economic revenue is one of many indicators that the current electrical grid is outdated. Not only must the grid become more reliable, it must also become more efficient, reduce its impact on the environment, incorporate alternative energy sources, allow for more consumer choices, and ensure cyber security. In effect, it must become “smart.” Significant investments in the billions of dollars are being made to lay the infrastructure of the future Smart Grid. However, the authors argue that we must take great care not to sacrifice consumer privacy amidst an atmosphere of unbridled enthusiasm for electricity reform. Information proliferation, lax controls and insufficient oversight of this information could lead to unprecedented invasions of consumer privacy. Smart meters and smart appliances will constitute a data explosion of intimate details of daily life, and it is not yet clear who will have access to this information beyond a person’s utility provider. The authors of this paper urge the adoption of Dr. Ann Cavoukian’s conceptual model ‘SmartPrivacy’ to prevent potential invasions of privacy while ensuring full functionality of the Smart Grid. SmartPrivacy represents a broad arsenal of protections, encapsulating everything necessary to ensure that all of the personal information held by an organization is appropriately managed. These include: Privacy by Design; law, regulation and independent oversight; accountability and transparency; market forces, education and awareness; audit and control; data security; and fair information practices. Each of these elements is important, but the concept of Privacy by Design represents its sine qua non. When applying SmartPrivacy to the Smart Grid, not only will the grid be able to, for example, become increasingly resistant to attack and natural disasters—it will be able to do so while also becoming increasingly resistant to data leakage and breaches of personal information. The authors conclude that SmartPrivacy must be built into the Smart Grid during its current nascent stage, allowing for both consumer control of electricity consumption and consumer control of their personal information, which must go hand in hand. Doing so will ensure that consumer confidence and trust is gained, and that their participation in the Smart Grid contributes to the vision of creating a more efficient and environmentally friendly electrical grid, as well as one that is protective of privacy. This will result in a positive-sum outcome, where both environmental efficiency and privacy can coexist.
Reusable and highly active supported copper(i)–NHC catalysts for Click chemistry (2013)

Immobilised [Cu(NHC)] catalysts are reported for the preparation of 1,2,3-triazoles. In addition to showing outstanding catalytic activity, the catalyst systems are easy to prepare and can be recycled many times.
Cellular Localization of Latent Murine Cytomegalovirus (1998)

Herpesviruses typically establish latent infection in their hosts. The cell(s) responsible for harboring latent virus, in most cases, is not known. Using immunofluorescence and PCR-in situ hybridization (PISH), a technique which combines the sensitivity of PCR with the localization and specificity of in situ hybridization, we provide the first direct evidence that endothelial cells are a major site of murine cytomegalovirus (MCMV) DNA in latently infected animals. These findings are consistent with existing knowledge of the biological behavior of CMV, in particular the transmission of latent CMV by solid organ and bone marrow transplantation, in both human and animal models. In addition, we have localized MCMV DNA in the lung alveolar macrophage and in bone marrow cells. Our findings confirm that bone marrow-derived hematopoietic cells are a site of CMV latency and further suggest that bone marrow may be a reservoir of infected progeny capable of migrating into the circulation and establishing latency in various tissues. These findings provide clearly needed insight into the site of latent infection which is central to an understanding of the mechanisms of reactivation.
Repeated Intracerebroventricular Administration of Glucagon-Like Peptide-1-(7–36) Amide or Exendin-(9–39) Alters Body Weight in the Rat**This work was supported by the United Kingdom Medical Research Council. (1999)

Central nervous system glucagon-like peptide-1-(7-36) amide (GLP-1) administration has been reported to acutely reduce food intake in the rat. We here report that repeated intracerebroventricular (i.c.v.) injection of GLP-1 or the GLP-1 receptor antagonist, exendin-(9-39), affects food intake and body weight. Daily i.c.v. injection of 3 nmol GLP-1 to schedule-fed rats for 6 days caused a reduction in food intake and a decrease in body weight of 16 +/- 5 g (P < 0.02 compared with saline-injected controls). Daily i.c.v. administration of 30 nmol exendin-(9-39) to schedule-fed rats for 3 days caused an increase in food intake and increased body weight by 7 +/- 2 g (P < 0.02 compared with saline-injected controls). Twice daily i.c.v. injections of 30 nmol exendin-(9-39) with 2.4 nmol neuropeptide Y to ad libitum-fed rats for 8 days increased food intake and increased body weight by 28 +/- 4 g compared with 14 +/- 3 g in neuropeptide Y-injected controls (P < 0.02). There was no evidence of tachyphylaxis in response to i.c.v. GLP-1 or exendin-(9-39). GLP-1 may thus be involved in the regulation of body weight in the rat.