gebhart/patrepeval huggingface dataset:
Diversification of the Structural Determinants of Fibroblast Growth Factor-Heparin Interactions (2012)

The functions of a large number (>435) of extracellular regulatory proteins are controlled by their interactions with heparan sulfate (HS). In the case of fibroblast growth factors (FGFs), HS binding determines their transport between cells and is required for the assembly of high affinity signaling complexes with their cognate FGF receptor. However, the specificity of the interaction of FGFs with HS is still debated. Here, we use a panel of FGFs (FGF-1, FGF-2, FGF-7, FGF-9, FGF-18, and FGF-21) spanning five FGF subfamilies to probe their specificities for HS at different levels as follows: binding parameters, identification of heparin-binding sites (HBSs) in the FGFs, changes in their secondary structure caused by heparin binding and structures in the sugar required for binding. For interaction with heparin, the FGFs exhibit K(D) values varying between 38 nM (FGF-18) and 620 nM (FGF-9) and association rate constants spanning over 20-fold (FGF-1, 2,900,000 M(-1) s(-1) and FGF-9, 130,000 M(-1) s(-1)). The canonical HBS in FGF-1, FGF-2, FGF-7, FGF-9, and FGF-18 differs in its size, and these FGFs have a different complement of secondary HBS, ranging from none (FGF-9) to two (FGF-1). Differential scanning fluorimetry identified clear preferences in these FGFs for distinct structural features in the polysaccharide. These data suggest that the differences in heparin-binding sites in both the protein and the sugar are greatest between subfamilies and may be more restricted within a FGF subfamily in accord with the known conservation of function within FGF subfamilies.
Influence of Organic Phosphonates on Hydroxyapatite Crystal Growth Kinetics (1996)

Phosphonate additives have important applications both in biomineralization processes and in industrial mineral scale formation. The kinetics of crystal growth of hydroxyapatite (HAP) has been investigated in the presence of seven phosphonate additives: HEDP (hydroxyethylenediphosphonic acid), DETPMP (diethylenetriaminepenta(methylenephosphonic acid)), DHTPMP (dihexyltriaminepenta (methylenephosphonic acid)), AMP (aminotris(methylenephosphonic acid)), HDTMP (hexyldiaminetetra(methylenephosphonic acid)), EDTMP (ethylenediaminetetra(methylenephosphonic acid)), and DETMP (diaminoethoxytetra(methylenephosphonic acid)). The constant composition (CC) method, used in this study, enabled reliable crystal growth rate data to be obtained even when the reactions were appreciably inhibited. The experiments were performed at pH 7.40, ionic strength 0.15 mol L-1 (maintained with NaCl), and an HAP relative supersaturation, σ, of 5.5. The results indicate that traces of some phosphonates (≤10-6 mol L-1) are extremely effective in inhibiting crystal growth. Assuming that the adsorbed additives block discrete growth sites on the crystal surfaces, the kinetic results may be interpreted in terms of a Langmuir adsorption model yielding kinetic affinity constants. The order of inhibitory effectiveness, DETMP > EDTMP > DETPMP > HEDP ≥ DHTPMP > HDTMP ≥ AMP, reflects the ability of the phosphonates to bind to the apatite surfaces. ζ potential measurments of HAP surfaces in the presence of additives provide important corroboratory data for the interpretation of the crystal growth results.
Wideband array antenna concept (2006)

This paper presents a unique wideband array concept that includes a wideband array aperture incorporating radiating elements of different sizes and inter-element spacings. The array is a fully-filled aperture consisting of a core region surrounded by several layers of radiating elements which increase in size. By proper selection of the core region and the number of layers, one can form a beam with specified beamwidth over a five to one frequency range. The proposed concept considerably reduces the cost and complexity by decreasing the effective number of radiating elements and the corresponding receive or transmit modules. Numerical simulation results, ignoring the mutual coupling effects, are presented for array patterns at several frequencies to prove the concept. A possible beamforming concept is also included. Additional work is planned to prove the viability of the concept by taking the mutual coupling into account.
Monoclonal Antibodies to DIPA: A Novel Binding Partner of p120-Catenin Isoform 1 (2012)

The coiled-coil domain-containing delta-interacting protein A (DIPA) is a transcription factor implicated in developmental regulation. DIPA is the first protein discovered to selectively interact with the p120-catenin (p120) isoform 1, an alternatively spliced form of p120 expressed preferentially in mesenchymal cells. Although a small fraction of p120 can be observed in the nucleus under certain circumstances, the vast majority of it associates with classical cadherins at adherens junctions. We observed for the first time that a discrete fraction of DIPA exists at cell-cell junctions, in addition to its predominantly nuclear localization. Thus, the p120-DIPA interaction may regulate cell signaling and/or transcriptional events, as has been described previously for β-catenin and the LEF/TCF transcription factor family. To facilitate further study of DIPA and to determine the physiological relevance of its interaction with p120, we have generated and characterized a panel of five DIPA-specific monoclonal antibodies (MAbs) that function in immunoblotting, immunoprecipitation, and immunofluorescence assays.
Model Predictive Control for Path Tracking of a VTOL Tailsitter UAV in an HIL Simulation Environment (2018)

2017-2018 > Academic research: refereed > Refereed conference paper
Instrumentation and method for high accuracy geo-referencing of sugar beet plants (2007)

Efficient use of automatic field operations will allow care and management of crops in very different systems from what is known today and may lead to the possibility of individual plant care systems. Automatic field operation systems have the potential to reduce environmental impact while preserving economics in crop production. These systems require accurate and reliable information about the position of individual crop plants and, if possible, additional information about crop growth status. The aim of the presented research was to generate a geo-spatial map of individual crop plants derived from geo-referenced data recorded during seeding operation. A standard sugar beet precision seeder was retrofitted with optical sensors that could detect seeds as they were released into the furrow. Furthermore, a real time kinematic global positioning system (RTK-GPS) and a dual axis tilt sensor provided the global position and attitude angles of the seeder. A data acquisition system was configured for recording and storage of global positions, seeder attitude data, and seed drop detections during seeding. This paper outlines the methodology of processing the recorded data into a geo-spatial seed map. The developed instrumentation was used in field experiments under typical conditions and operation velocities up to 5.3 km h−1. The validation showed that 95% of the sugar beet seedlings emerged within 37.3 mm from the seed drop positions contained in the geo-spatial seed map. An error analysis associated with the estimation of geo-referenced plant positions consisted of positioning sensor errors, seed displacement in the furrow and deviation between location of plant emergence and the corresponding seed location. Furthermore, the error contribution from individual sources was of the same magnitude, except for the error due to deviation between location of plant emergence and the corresponding seed location. As this fully random error will always occur, it was considered meaningless trying to further minimize the sensor errors. Inclusion of seeder attitude data in the data processing significantly improved the accuracy of the estimation of geo-referenced plant positions and therefore it was concluded that a dual axis tilt sensor should be a required part of the instrumentation. Furthermore, it was shown that high accuracy of the estimation of geo-referenced plant positions required a zero horizontal velocity of the seed released from the seeding mechanism. In general, the overall accuracy of the estimation of geo-referenced plant positions was satisfactory to allow subsequent individual plant scale operations.
Simultaneous determination of gemfibrozil and its metabolites in plasma and urine by a fully automated high performance liquid chromatographic system (1991)

Sensitive and specific methods for the simultaneous determination of gemfibrozil (Lopid), a lipid-lowering agent, and its metabolites in plasma and urine are described. The methods are based on a fully automated high performance liquid chromatographic (HPLC) system with fluorescence detection. Urine samples, diluted with acetonitrile, were directly analysed by HPLC using a flow and eluent programming method. In the case of plasma, gemfibrozil and its main metabolites were extracted from acidified samples and the resulting extracts injected into the chromatographic system. The sensitivity was approximately 100 ng/mL for gemfibrozil and its four metabolites using 0.5 mL plasma or urine. An acyl glucuronide of gemfibrozil excreted in human urine after oral administration of the drug was isolated and its structure and stability examined.
A New Approach for the Camera Motion Extraction from Texture Images with Spatio-Temporal Slice Analysis (2009)

It is difficult to extract the motion information between the camera and the object surface with the model matching or the optical flow method when there is only texture structure on the object surface for observing. Arranging the spatio-temporal images with the time order and cutting the images array along a row or a column, a spatio-temporal slice image appears. Different camera motion reaches different slice image and the camera motion information can be extracted by analyzing this kind of slice images. Differ from the common spatio-temporal slice analysis methods, a measurement parameter to describe the texture elements distribute density is present in this paper. Camera motion information can be extracted by analyzing this parameter in spatio-temporal slices of different positions.
Studies on Tocopherol Derivatives. I. Conversion of β-, γ-, and δ-Tocopherol to α-Tocopherol (1971)

A four-step methylation of β-, γ-, and δ-tocopherol to α-tocopherol is described. All of the intermediates are isolated and characterized. The purities of methylated tocopherols are determined by quantitative gas liquid chromatography. The four steps are Mannich reaction, acetolysis, reductive cleavage, and reduction.
Raised parenchymal interleukin-6 levels correlate with improved outcome after traumatic brain injury (2004)

Previous studies have suggested that an increased production of the pro-inflammatory cytokines interleukin-6 (IL-6) and interleukin-1beta (IL-1beta) can influence patient outcome following a severe head injury. However, these studies have relied upon measurements of cytokine levels in CSF or serum, rather than the brain parenchyma itself. Recently, a method of intracranial microdialysis has been developed which permits the efficient recovery of macromolecules from the parenchyma. We have used this technique to investigate whether there is a correlation between patient outcome and parenchymally derived cytokines. Fourteen patients who were admitted to the Wessex Neurological Centre with severe head injury were selected for the study. This group of patients consisted of seven males and seven females with an age range of 21-77 years. Patients were treated according to standard protocols including emergency craniotomy where necessary. Microdialysis probes were implanted into the frontal region contralateral to the site of the primary injury. Approximately 200 micro l of dialysate was recovered every 8-12 h, and the concentrations of IL-6, IL-1beta and nerve growth factor (NGF) were determined by commercial enzyme-linked immunosorbent assays. Patients were assessed initially using the Glasgow coma score, and survivors were assessed after 6 months using the Glasgow outcome scale. Significantly (P = 0.04) higher levels of IL-6 were found in patients who survived compared with those who died. Also, there was a significant correlation between peak IL-6 levels and Glasgow outcome scores (r(2) = 0.34, P = 0.03, n = 14). The levels of IL-1beta and NGF were similar in both groups of patients. From these data, we suggest that IL-6 is an endogenous neuroprotective cytokine produced in response to severe head trauma.
A novel approach to the synthesis of morphine alkaloids: the synthesis of (d,l)-thebainone-A (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA novel approach to the synthesis of morphine alkaloids: the synthesis of (d,l)-thebainone-AMarcus A. Tius and Michael A. KerrCite this: J. Am. Chem. Soc. 1992, 114, 15, 5959–5966Publication Date (Print):July 1, 1992Publication History Published online1 May 2002Published inissue 1 July 1992https://pubs.acs.org/doi/10.1021/ja00041a008https://doi.org/10.1021/ja00041a008research-articleACS PublicationsRequest reuse permissionsArticle Views1639Altmetric-Citations74LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Acrylic Acid-Based Copolymers as Functional Binder for Silicon/Graphite Composite Electrode in Lithium-Ion Batteries (2015)

Acrylic acid-based copolymers are synthesized by polymerization of acrylic acid with a small portion of crosslinker, diallyl ethers. The obtained copolymers are soluble in water, and viscosity of their aqueous solution incrementally varies with increase in the amount of crosslinker and neutralization degree of -COOH groups with NaOH. The copolymers are examined as binders for silicon/graphite composite electrodes for lithium-ion batteries. Reversibility and lithiation capacity of the composite are highly improved by the selection of copolymers, which is due to better homogeneity and mechanical durability of the composite electrodes.
Formal Substitution of Bromocyclopropanes with Nitrogen Nucleophiles (2013)

A highly chemo- and diastereoselective protocol toward amino-substituted donor-acceptor cyclopropanes via the formal nucleophilic displacement in bromocyclopropanes is described. A wide range of N-nucleophiles, including carboxamides, sulfonamides, azoles, and anilines, can be efficiently employed in this transformation, providing expeditious access to stereochemically defined and densely functionalized cyclopropylamine derivatives.
Optimizing trichromacy for information about surface color in natural scenes (2005)

Color vision may be used to help identify objects and segment scenes despite variations in the spectral composition of the light. But humans, and some animals, have just three classes of cone receptors, too few to fully specify spectra. Given this limit, do normal human cones maximize the information available? This question was addressed by the present study. Images of rural and urban scenes were obtained with a high-resolution hyperspectral imaging system, which provided estimates of surface spectral reflectance over 400–720 nm at 10-nm intervals at each point in a digital representation of spatial resolution 1344 × 1024 pixels. In computational simulations, 35 scenes were illuminated by randomly selected combinations of daylights with and without filtering through a leafy canopy. Five thousand points in each scene were randomly selected and Shannon's mutual information was calculated as a function of the spectral position of a variable number of cone pigments. The mutual information had several maxima, three of the highest being within about 10 nm of the mean normal long-, medium-, and short-wave cone positions, suggesting that trichromacy is almost optimal for extracting information about surface color in natural scenes.
Defluorination of Perfluoropropene Using Cp*<sub>2</sub>ZrH<sub>2</sub>and Cp*<sub>2</sub>ZrHF:  A Mechanism Investigation from a Joint Experimental−Theoretical Perspective (2004)

Cp*(2)ZrH(2) (1) (Cp* = pentamethylcyclopentadienyl) reacts with perfluoropropene (2) to give Cp*(2)ZrHF (3) and hydrodefluorinated products under very mild conditions. Initial C-F bond activation occurs selectively at the vinylic terminal position of the olefin to exchange fluorine for hydrogen. Subsequent hydrodefluorination leads to the formation of the n-propylhydride complex Cp*(2)ZrH(CH(2)CH(2)CH(3)), which can be cleaved with dihydrogen to give propane and 1. A theoretical study of the reaction of Cp*(2)ZrH(2) (Cp* = cyclopentadienyl) and CF(2)[double bond]CF(CF(3)) has been undertaken. Several mechanisms have been examined in detail using DFT(B3PW91) calculations and are discussed for this H/F exchange: (a) internal olefin insertion/beta-fluoride elimination, (b) external olefin insertion/beta-fluoride elimination, and (c) F/H metathesis from either an inside or outside approach. Of these, the first case is found to be energetically preferred. Selective defluorination at the terminal carbon has been shown to be favored over defluorination at the substituted and allylic carbons.
Evaluation of MTBE Gasoline by Japanese Passenger Cars (1980)

<div class="htmlview paragraph">Performance and the compatibility of methyl tertiary butyl ether (MTBE) as a blending component for motor gasolines were assessed for Japanese passenger cars.</div> <div class="htmlview paragraph">MTBE was found to be an excellent road octane booster. But fuel consumption and exhaust emissions of MTBE-containing gasoline were observed to differ from conventional gasoline. The addition of MTBE changed the equivalence ratio of the mixture and reduced the calorific value of the fuel.</div> <div class="htmlview paragraph">Engine cleanliness, ORI and crankcase oil deterioration were not influenced by MTBE gasoline.</div> <div class="htmlview paragraph">MTBE gasoline was compatible with the elastomers and metals used for fuel system of Japanese passenger cars and was found to behave similarly to conventional gasoline.</div> <div class="htmlview paragraph">From these results, MTBE was judged to be an excellent octane appreciator in motor fuel for Japanese passenger cars.</div>
Extracellular Carbohydrate-Containing Polymers of a Model Biofilm-Producing Strain, <i>Staphylococcus epidermidis</i> RP62A (2005)

Staphylococcus aureus and coagulase-negative staphylococci, primarily Staphylococcus epidermidis, are recognized as a major cause of nosocomial infections associated with the use of implanted medical devices. It has been established that clinical isolates often produce a biofilm, which is involved in adherence to biomaterials and provides enhanced resistance of bacteria against host defenses and antibiotic treatments. It has been thought that the staphylococcal biofilm contains two polysaccharides, one responsible for primary cell adherence to biomaterials (polysaccharide/adhesin [PS/A]) and an antigen that mediates bacterial aggregation (polysaccharide intercellular adhesin [PIA]). In the present paper we present an improved procedure for preparation of PIA that conserves its labile substituents and avoids contamination with by-products. Based on structural analysis of the polysaccharide antigens and a thorough overview of the previously published data, we concluded that PIA from S. epidermidis is structurally identical to the recently described poly-beta-(1-->6)-N-acetylglucosamine from PS/A-overproducing strain S. aureus MN8m. We also show that another carbohydrate-containing polymer, extracellular teichoic acid (EC TA), is an essential component of S. epidermidis RP62A biofilms. We demonstrate that the relative amounts of extracellular PIA and EC TA produced depend on the growth conditions. Moderate shaking or static culture in tryptic soy broth favors PIA production, while more EC TA is produced in brain heart infusion medium.
Automatic Analysis of the Patient's Conscious Responses to the Emission of Auditory Stimuli during the Performance of an Audiometry (2011)

This paper presents a methodology for the automatic detection of human conscious responses to different auditory stimuli during the performance of an audiogram. An audiogram is the standard way of analyze the hearing of a patient in order to diagnose hearing loss. From a video sequence captured during the hearing evaluation performed by an expert to a patient, the methodology will be able to detect the instants in which the expert is sending auditory stimuli and when the patient responds consciously to them by raising his hand in order to indicate that he has heard them. The proposed method has been tested on a dataset of almost 7000 frames from three different video sequences obtaining very encouraging results in the hand raise detection as a response to the auditory stimuli. Finally, an analysis of the patient's speed reaction to stimuli is discussed.
Quantitation of renal function with technetium-99m MAG3. (1988)

The technetium-labeled hippuran analog [99mTc]MAG3 was compared with [131I]hippuran in 50 patients using a quantitative renal function protocol that includes: (a) estimation of effective renal plasma flow by a single-injection, single-sample plasma clearance method, (b) determination of relative function of right and left kidney from the initial count rate over each kidney, and (c) comparison of recovered urine activity with plasma disappearance. This protocol is suitable for routine clinical use, and, in fact, has been used heavily at our clinic for a number of years. By slight modification of the formulas, the results obtained with [99mTc]MAG3 agreed well with those using [131I]hippuran. We conclude that [99mTc]MAG3 can be substituted for [131I]hippuran in the quantitative protocol, with the better image quality and lower radiation dose (in abnormals) of a technetium-labeled agent.
AlGaAs/GaAs HBT limiting amplifier for 10 Gbps optical transmission system (2002)

A 10 Gbit/s limiting amplifier IC for optical transmission system was implemented with AlGaAs HBT technology. Amplifier was designed to support differential input and output. Small signal performance of the packaged IC achieves 26 dB gain and f/sub 3dB/ of 8 GHz. A single output has 800 mV/sub p-p/ swing with more than 26 dB dynamic range. The performance of the limiting amplifier was verified through single mode fiber 320 km transmission link test.