gebhart/patrepeval huggingface dataset:
RhoB Protects Human Keratinocytes from UVB-induced Apoptosis through Epidermal Growth Factor Receptor Signaling (2005)

Exposure of the skin to UVB light results in the formation of DNA photolesions that can give rise to cell death, mutations, and the onset of carcinogenic events. Specific proteins are activated by UVB and then trigger signal transduction pathways that lead to cellular responses. An alteration of these signaling molecules is thought to be a fundamental event in tumor promotion by UVB irradiation. RhoB, encoding a small GTPase has been identified as a DNA damage-inducible gene. RhoB is involved in epidermal growth factor (EGF) receptor trafficking, cytoskeletal organization, cell transformation, and survival. We have analyzed the regulation of RhoB and elucidated its role in the cellular response of HaCaT keratinocytes to relevant environmental UVB irradiation. We report here that the activated GTP-bound form of RhoB is increased rapidly within 5 min of exposure to UVB, and then RhoB protein levels increased concomitantly with EGF receptor (EGFR) activation. Inhibition of UVB-induced EGFR activation prevents RhoB protein expression and AKT phosphorylation but not the early activation of RhoB. Blocking UVB-induced RhoB expression with specific small interfering RNAs inhibits AKT and glycogen synthase kinase-3beta phosphorylation through inhibition of EGFR expression. Moreover, down-regulation of RhoB potentiates UVB-induced cell apoptosis. In contrast, RhoB overexpression protects keratinocytes against UVB-induced apoptosis. These results indicated that RhoB is regulated upon UVB exposure by a two-step process consisting of an early EGFR-independent RhoB activation followed by an EGFR-dependent induction of RhoB expression. Moreover, we have demonstrated that RhoB is essential in regulating keratinocyte cell survival after UVB exposure, suggesting its potential role in photocarcinogenesis.
Mechanisms of WNK1 and WNK4 interaction in the regulation of thiazide-sensitive NaCl cotransport (2005)

With-no-lysine (WNK) kinases are highly expressed along the mammalian distal nephron. Mutations in either WNK1 or WNK4 cause familial hyperkalemic hypertension (FHHt), suggesting that the protein products converge on a final common pathway. We showed previously that WNK4 downregulates thiazide-sensitive NaCl cotransporter (NCC) activity, an effect suppressed by WNK1. Here we investigated the mechanisms by which WNK1 and WNK4 interact to regulate ion transport. We report that WNK1 suppresses the WNK4 effect on NCC activity and associates with WNK4 in a protein complex involving the kinase domains. Although a kinase-dead WNK1 also associates with WNK4, it fails to suppress WNK4-mediated NCC inhibition; the WNK1 kinase domain alone, however, is not sufficient to block the WNK4 effect. The carboxyterminal 222 amino acids of WNK4 are sufficient to inhibit NCC, but this fragment is not blocked by WNK1. Instead, WNK1 inhibition requires an intact WNK4 kinase domain, the region that binds to WNK1. In summary, these data show that: (a) the WNK4 carboxyl terminus mediates NCC suppression, (b) the WNK1 kinase domain interacts with the WNK4 kinase domain, and (c) WNK1 inhibition of WNK4 is dependent on WNK1 catalytic activity and an intact WNK1 protein. These findings provide insight into the complex interrelationships between WNK1 and WNK4 and provide a molecular basis for FHHt.
Spatial coupling in heterogeneous catalysis (1995)

Spatial coupling mechanisms are studied in the heterogeneous catalytic oxidation of carbon monoxide over platinum at atmospheric pressure under oscillatory conditions. Experiments are conducted in a continuous flow reactor, and the reaction rate is monitored using both infrared imaging and thermocouples. The catalysts are in the form of platinum annular thin films on washer-shaped quartz substrates, and they provide highly repeatable oscillatory behavior. Oscillations are typically spatially synchronized with the entire catalyst ‘‘flashing’’ on and off uniformly. Spatial coupling is investigated by introducing various barriers which split the annular ring in half. Infrared images show that coupling through the gas phase dominates coupling via the diffusion of CO on the surface or heat diffusion through the substrate. The introduction of a localized heat perturbation to the catalyst surface does not induce a transition in the reaction rate. Thus, it is likely that the primary mode of communication is through the gas-phase diffusion of reactants.
Redox Ionic Liquid Phases:  Ferrocenated Imidazoliums (2006)

This communication reports the synthesis of new intrinsically electroactive N-alkyl imidazolium ionic liquids useful for the investigation of charge transport phenomena in undiluted semisolid redox media. The redox site chosen is ferrocene, but the overall approach should be general for designing imidazolium-based molten salts. The ferrocene is attached to imidazolium with several structural variations, and preliminary voltammetry of a melt is presented.
Coherent Power Combining of Chirped-Seed Erbium-Doped Fiber Amplifiers (2013)

Using a rapidly-chirped <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$(5\times 10^{14}~{\rm Hz}/{\rm s})$</tex> </formula> continuous-wave optoelectronic swept-frequency seed laser and two 3 W erbium-doped fiber amplifiers, we demonstrate simultaneous stimulated Brillouin scattering suppression, coherent power combining, and electronic beam steering, without imposing strict path-length matching requirements. Our platform presents a viable path toward high-power continuous-wave sources.
Calcium‐Independent Release of Acetylcholine from Stable Cell Lines Expressing Mouse Choline Acetyltransferase cDNA (1994)

Stably transfected cells expressing mouse choline acetyltransferase (ChAT) cDNA were established, and the synthesis and release of acetylcholine (ACh) were examined. A cDNA clone coding for mouse ChAT was inserted into an expression vector (pEF321) containing a promoter for human elongation factor 1 alpha to construct pEFmChAT. Neuronal (NG108-15, NS20Y, N1E115, and Neuro2A) and nonneuronal cell lines (L cells and NIH3T3) were transfected with pEFmChAT, and the cell lines that stably expressed high ChAT activity were selected. These cells expressed the 66-kDa ChAT protein and accumulated ACh mostly in the cytosol. The concentration of intracellular ACh in the cells increased upon raising the choline level in the medium. The cells continuously released ACh in a Ca(2+)-independent fashion. Neither high K+ nor calcium ionophore stimulated release of ACh from the cells.
Medullary catecholaminergic neurons in the normal human brain and in Parkinson's disease (1991)

Parkinson's disease is thought to cause degeneration of melanin-pigmented catecholaminergic neurons throughout the brainstem, but little quantitative information is available on the fate of catecholaminergic neurons associated with the dorsal vagal complex or medullary reticular formation. We therefore examined these neurons in the normal human medulla and in the brains of patients with Parkinson's disease, using both a melanin stain and immunohistochemical methods with an antiserum against tyrosine hydroxylase. The greatest numbers of catecholaminergic neurons in the ventrolateral reticular formation (A1/C1 group) were located in the far rostral medulla, whereas the largest populations of catecholaminergic cells in the dorsal vagal complex (A2/C2 group) were found at the level of the area postrema. No loss of cells was observed in the A1/C1 group in the parkinsonian brains. In contrast, the A2/C2 group showed moderate loss of neurons, most marked at the level of the area postrema. This difference was entirely due to the loss of neurons in the medial component of the A2 group, a population that normally is only lightly pigmented, while the heavily pigmented neurons in the ventral and intermediate components of the A2 complex were unaffected. Parkinson's disease causes degeneration only of selected populations of medullary catecholaminergic neurons, without apparent relationship to the extent of melanin pigmentation.
Using Cooperative Adaptive Cruise Control (CACC) to Form High-Performance Vehicle Streams (2014)

This research identifies the operational concepts for managing cooperative adaptive cruise control (CACC) vehicle maneuvering and traffic flows. This includes approaches for grouping the CACC vehicles, ranging from ad-hoc to centrally coordinated strategies, and the incentives that could be used to facilitate the vehicle clustering, both operational and financial. These are particularly important at low market penetrations, when the CACC vehicles are likely to be widely separated. The dissolution of CACC strings is also discussed, since this needs to be done carefully to avoid adverse traffic impacts. While the main focus is on vehicle-to-vehicle (V2V) CACC for use on limited access highways, strategies for infrastructure to vehicle (I2V) CACC and for both V2V and I2V CACC on signalized arterials are also considered. Connected Cruise Control (CCC), which has been developed as a driver-advisory transitional strategy to lead toward CACC in the Netherlands, is also discussed.
Exfocal Pumping of Optical Masers in Elliptical Mirrors (1964)

An efficiency near unity can be achieved in optical pumping devices for lasers, where unabsorbed light is refocused at the light source, leading to multiple passes of pumping energy through source and laser. In properly dimensioned elliptical mirrors this can, in principle, be done by placing source and laser outside of the focal points. Furthermore, in these “exfocal” elliptical designs the light density of the source is transformed into the laser at a ratio of 1:1, resulting in very low-threshold energies. The lowest value observed for ruby lasers up to 7.6 cm in length was 50 W-sec at room temperature in a rotational-ellipsoidal mirror where the axis of source and laser are oriented in the rotational axis of the ellipsom, while their lengths are equal to the distance between focus and wall. Alternative designs are exfocal elliptical and circular cylinders. In exfocal ellipsoids the pump light distribution is of exactly rotational symmetry which leads to symmetrical absorption of pumping light in the laser. As a result, quasi-periodic relaxation oscillations of 5000-μsec duration and a component of continuous emission have been observed at room temperature with 300 W-sec pumping energy.
Fundamental Principles of Condensation Polymerization. (1946)

ADVERTISEMENT RETURN TO ISSUEPREVArticleFundamental Principles of Condensation Polymerization.Paul J. FloryCite this: Chem. Rev. 1946, 39, 1, 137–197Publication Date (Print):August 1, 1946Publication History Published online1 May 2002Published inissue 1 August 1946https://pubs.acs.org/doi/10.1021/cr60122a003https://doi.org/10.1021/cr60122a003research-articleACS PublicationsRequest reuse permissionsArticle Views4070Altmetric-Citations499LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Phase I Trial of Intranasal and Endobronchial Administration of a Recombinant Adeno-Associated Virus Serotype 2 (rAAV2)-CFTR Vector in Adult Cystic Fibrosis Patients: A Two-Part Clinical Study (2003)

Recombinant adeno-associated serotype 2-based vectors (rAAV2) possess a number of theoretical advantages for cystic fibrosis (CF) gene therapy because they elicit little or no inflammatory response and generally result in stable expression. rAAV2 vectors expressing the cystic fibrosis transmembrane conductance regulator (CFTR) gene have previously been shown to mediate stable correction of the CF defect in CF bronchial epithelial cells and stable expression of CFTR in rabbit and nonhuman primate models. Here we report the results of the first trial initiated with rAAV in humans, a phase I study in 25 adult and adolescent CF patients with mild to moderate lung disease. Doses of the rAAV-CFTR vector (tgAAVCF) ranging from 3 x 10(1) to 1 x 10(9) replication units (RU), which is equivalent to approximately 6 x 10(4) to 2 x 10(12) DNase resistant particles (DRP), were administered to one side of the nose and to the superior segment of the lower lobe of the right lung. Several adverse events were noted prior to and/or after vector delivery, but most of them appeared to be related to the endogenous CF lung disease or a result of the bronchoscopic procedures. Only one of the serious events was judged to be possibly vector-related (based on temporal association), and this event was a pulmonary exacerbation very similar to several others experienced by the same subject in the three months preceding vector delivery. Vector shedding was minimal throughout the study, and serum-neutralizing antibodies were detected after vector delivery to subjects in the highest dosage cohorts. Gene transfer as measured by DNA polymerase chain reaction (PCR) was not observed until cohort 10 in nasal and bronchial epithelia. Sporadic low-level copy numbers suggested gene transfer of anywhere from 0.002 copies per cell up to 0.5 copies per cell was possible; however, DNA PCR was positive in lungs prior to direct dosing suggesting aspiration from the nasal dosing. These data indicate the need for continued evaluation of rAAV-CFTR vectors in additional clinical trials.
Notes on Lubricating Greases Made from the Soaps of Phenyl-Stearic,Acid (1930)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNotes on Lubricating Greases Made from the Soaps of Phenyl-Stearic,AcidWilliam GilfoilCite this: Ind. Eng. Chem. 1930, 22, 5, 487Publication Date (Print):May 1, 1930Publication History Published online1 May 2002Published inissue 1 May 1930https://pubs.acs.org/doi/10.1021/ie50245a016https://doi.org/10.1021/ie50245a016research-articleACS PublicationsRequest reuse permissionsArticle Views18Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
From clinician to manager (1980)

Abstract New skills must be learned and a new self‐image must be developed by a clinician who assumes a middle management role for the first time.
A Gaussian process regression approach to a single-index model (2010)

We consider a Gaussian process regression (GPR) approach to analysing a single-index model (SIM) from the Bayesian perspective. Specifically, the unknown link function is assumed to be a Gaussian process a priori and a prior on the index vector is considered based on a simple uniform distribution on the unit sphere. The posterior distributions for the unknown parameters are derived, and the posterior inference of the proposed approach is performed via Markov chain Monte Carlo methods based on them. Particularly, in estimating the hyperparameters, different numerical schemes are implemented: fully Bayesian methods and empirical Bayes methods. Numerical illustration of the proposed approach is also made using simulation data as well as well-known real data. The proposed approach broadens the scope of the applicability of the SIM as well as the GPR. In addition, we discuss the theoretical aspect of the proposed method in terms of posterior consistency.
Improved Laplacian Smoothing of Noisy Surface Meshes (1999)

This paper presents a technique for smoothing polygonal surface meshes that avoids the well‐known problem of deformation and shrinkage caused by many smoothing methods, like e.g. the Laplacian algorithm. The basic idea is to push the vertices of the smoothed mesh back towards their previous locations. This technique can be also used in order to smooth unstructured point sets, by reconstructing a surface mesh to which the smoothing technique is applied. The key observation is that a surface mesh which is not necessarily topologically correct, but which can efficiently be reconstructed, is sufficient for that purpose.
A unipolar pulse electromagnetic field apparatus for magnetic therapy (2018)

The National Aeronautics and Space Administration (NASA) has spent years studying magnetic therapy and has built their own pulse electromagnetic field apparatus (PEMF). The concept of unipolar pulsed electromagnetic field (UPEMF) devices is presented; this device is an improved medical apparatus for magnetic therapy based on PEMF. A concentrator is designed to strengthen the magnetic field on the north pole and weaken the opposite field. The UPEMF device provides an approximate unipolar electromagnetic field and meets the medical requirements of magnetic therapy in terms of frequency, shape, and intensity.
Deletion of Microfibrillar‐Associated Protein 4 Attenuates Left Ventricular Remodeling and Dysfunction in Heart Failure (2020)

Background Cardiac remodeling predisposes individuals to heart failure if the burden is not solved, and heart failure is a growing cause of morbidity and mortality worldwide. The cardiac extracellular matrix not only provides structural support, but also is a core aspect of the myocardial response to various biomechanical stresses and heart failure. MFAP4 (microfibrillar-associated protein 4) is an integrin ligand located in the extracellular matrix, whose biological functions in the heart remain poorly understood. In the current study we aimed to test the role of MFAP4 in cardiac remodeling. Methods and Results MFAP4-deficient (MFAP4<sup>-/-</sup>) and wild-type mice were subjected to aortic banding surgery and isoproterenol to establish models of cardiac remodeling. We also evaluated the functional effects of MFAP4 on cardiac hypertrophy, fibrosis, and cardiac electrical remodeling. The expression of MFAP4 was increased in the animal cardiac remodeling models induced by pressure overload and isoproterenol. After challenge of 8 weeks of aortic banding or 2 weeks of intraperitoneal isoproterenol, MFAP4<sup>-/-</sup> mice exhibited lower levels of cardiac fibrosis and fewer ventricular arrhythmias than wild-type mice. However, there was no significant effect on cardiomyocyte hypertrophy. In addition, there was no significant difference in cardiac fibrosis severity, hypertrophy, or ventricular arrhythmia incidence between wild-type-sham and knockout-sham mice. Conclusions These findings are the first to demonstrate that MFAP4 deficiency inhibits cardiac fibrosis and ventricular arrhythmias after challenge with 8 weeks of aortic banding or 2 weeks of intraperitoneal isoproterenol but does not significantly affect the hypertrophy response. In addition, MFAP4 deficiency had no significant effect on cardiac fibrosis, hypertrophy, or ventricular arrhythmia in the sham group in this study.
Synergism within polyhexamethylene biguanide biocide formulations (1990)

Polyhexamethylene biguanides (PHMB) are mixtures of polymeric biguanides with an average polymer length (n) of 5, but containing high (n greater than 15, mol. wt 3300) and low molecular weight material (n = 2, mol. wt 400). Studies involving discrete molecular weight fractions of PHMB have shown that antimicrobial activity of PHMB increases with increasing polymer length. Cell suspensions which had not been subjected to centrifugation and/or washing during their preparation were employed. Whilst activity was still observed to increase with n, the trend was much reduced as n exceeded six. Centrifugation and washing of cells markedly increased the activity of high but not low molecular weight materials and corresponded to losses upon centrifugation of envelope lipopolysaccharide (LPS). Such envelope LPS represented high affinity binding sites on the surfaces of the cells. Combinations of various molecular weight fractions of PHMB were evaluated against filter-washed cells and revealed a profound synergy between extremes of polymer length.
High Mutation Frequencies Among <i>Escherichia coli</i> and <i>Salmonella</i> Pathogens (1996)

Here it is reported that the incidence of mutators among isolates of pathogenic Escherichia coli and Salmonella enterica is high (over 1 percent). These findings counter the theory, founded on studies with laboratory-attenuated strains, that suggests mutators are rare among bacterial populations. Defects in methyl-directed mismatch repair underlie all mutator phenotypes described here. Of nine independently derived hypermutable strains, seven contained a defective mutS allele. Because these mutant alleles increase the mutation rate and enhance recombination among diverse species, these studies may help explain both the rapid emergence of antibiotic resistance and the penetrance of virulence genes within the prokaryotic community.
Non-linear view interpolation (1999)

A new non-linear view interpolation algorithm is presented in this paper. Unlike the linear interpolation scheme, our method can exactly simulate the perspective viewing transformation during walkthrough. To accelerate the view interpolation, the algorithm employs a binary subdivision scheme to optimize the decomposition of the source image so that the number of resultant blocks is greatly reduced. Holes in the intermediate image are filled by two steps, namely enlarging the transferred blocks at the sides adjacent to holes and retrieving the local image within holes from the destination images by multidirectional interpolation. Experimental results demonstrate that our algorithm is much more accurate and efficient than the traditional one. Copyright © 1999 John Wiley & Sons, Ltd.
Non-frozen preservation of committed hematopoietic stem cells from normal human bone marrow. (1981)

We examined the optimal conditions for maintaining normal human hematopoietic stem cells without cryopreservation. The effects of serum, HEPES, CPD and storage temperature were studied. Stem cell integrity (CFU-c, CFU-e, and BFU-e) was optimally maintained when the bone marrow cells were suspended in a medium containing 40% fetal calf serum, 25 mM HEPES, and 12.28% CPD and maintained at 4 degrees C. For example, under these storage conditions the recovery of functionally intact early erythroid stem cells, BFU-e, was 84% after 2 days, 44% after 4 days and 12% after 7 days. These results indicate that non-frozen short-term storage of human bone marrow may be of clinical utility in bone marrow transplantation.