gebhart/patrepeval huggingface dataset:
Isolation and characterization of a 35,000 molecular weight subunit fetal cartilage matrix protein. (1983)

A noncollagenous protein, which is a major component of the extracellular matrix of third trimester fetal epiphyseal cartilage, has been isolated and characterized.On sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, the protein exists in the form of its 35,000 molecular weight subunit.Under nonreducing conditions, the protein exists as a 69,000 molecular weight dimer.In the studies reported here, the protein was usually identified as its 35,000 molecular weight subunit, demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions.Noncollagenous proteins and proteoglycans were extracted in 4 M guanidine hydrochloride containing protease inhibitors from the epiphyseal cartilage of bovine fetuses ranging in age from 106 to 270 days.The noncollagenous proteins were separated from proteoglycans by equilibrium density gradient centrifugation under associative conditions.The fraction containing the extracted proteins was examined by sodium dode-cy1 sulfate-polyacrylamide gel electrophoresis under reducing conditions, to identify the protein species extracted from fetal epiphyseal cartilages of different ages.During the third trimester the 35,000 molecular weight matrix protein was the protein species present in highest concentration in extracts of fetal epiphyseal cartilage.The matrix protein was isolated by chromatography on: (a) DEAE-Sephacel in 6 M urea; (b) Affi-Gel blue; (c) hydroxyapatite; and (d) Sephacryl S-200 in 4 M guanidine hydrochloride.The isolated protein was characterized in terms of amino acid composition, carbohydrate composition and molecular weights under denaturing and nondenaturing conditions.An antiserum was prepared against the 35,000 molecular weight protein and its homogeneity and immunological identity were examined by double immunodiffusion, crossed immunoelectrophoresis, and ELISA.Cartilate extracellular matrix is composed of collagen, proteoglycans, and a variety of noncollagenous proteins and glycoproteins, few of which have been isolated and characterized.
A robust approach for the derivation of closed-form Green's functions (1996)

Spatial-domain Green's functions for multilayer, planar geometries are cast into closed forms with two-level approximation of the spectral-domain representation of the Green's functions. This approach is very robust and much faster compared to the original one-level approximation. Moreover, it does not require the investigation of the spectral-domain behavior of the Green's functions in advance to decide on the parameters of the approximation technique, and it can be applied to any component of the dyadic Green's function with the same ease.
Study of Intravesical Stick (IVS). I. Preparative Method and IVS Dissolution Examination of Active Ingredient (1985)

The bladder has functions to retain and excrete the urine, differing from other regions. Therefore, when drugs are infused in the bladder, they are excreted during urination, and it is difficult to keep them acting in the affected regions for a long period. We have been studying a drug with a prolonged effect in the bladder, thus enabling topical chemotherapy of bladder cancers and prevention of their reccurrence. This led to research and development work on the intravesical stick (IVS). IVS has a base consisting of witepsol H-15, E-85, polysorbate 80 and bleached beeswax, and mixed drugs in it uniformly, and is formed to the stick-shape, and this semi solid dosage form can be applied into the bladder via urethra. The specific gravity was 0.989-0.998 (25°C), and dysuria develops hardly because of the floating into urine. Moreover, in the dissolution examination of active ingredient, the differences of elution patterns were noted by the type of the prescription of the base, and the active ingredient was detected from the start of the dissolution up to 48-120 h. From the results, the usefulness of this drug was suggested as a prolonged effective one.
Breathing Analysis Using Thermal and Depth Imaging Camera Video Records (2017)

The paper is devoted to the study of facial region temperature changes using a simple thermal imaging camera and to the comparison of their time evolution with the pectoral area motion recorded by the MS Kinect depth sensor. The goal of this research is to propose the use of video records as alternative diagnostics of breathing disorders allowing their analysis in the home environment as well. The methods proposed include (i) specific image processing algorithms for detecting facial parts with periodic temperature changes; (ii) computational intelligence tools for analysing the associated videosequences; and (iii) digital filters and spectral estimation tools for processing the depth matrices. Machine learning applied to thermal imaging camera calibration allowed the recognition of its digital information with an accuracy close to 100% for the classification of individual temperature values. The proposed detection of breathing features was used for monitoring of physical activities by the home exercise bike. The results include a decrease of breathing temperature and its frequency after a load, with mean values -0.16 °C/min and -0.72 bpm respectively, for the given set of experiments. The proposed methods verify that thermal and depth cameras can be used as additional tools for multimodal detection of breathing patterns.
The <i>white</i> Gene of <i>Ceratitis capitata</i> : A Phenotypic Marker for Germline Transformation (1995)

Reliable germline transformation is required for molecular studies and ultimately for genetic control of economically important insects, such as the Mediterranean fruit fly (medfly) Ceratitis capitata. A prerequisite for the establishment and maintenance of transformant lines is selectable or phenotypically dominant markers. To this end, a complementary DNA clone derived from the medfly white gene was isolated, which showed substantial similarity to white genes in Drosophila melanogaster and other Diptera. It is correlated with a spontaneous mutation causing white eyes in the medfly and can be used to restore partial eye color in transgenic Drosophila carrying a null mutation in the endogenous white gene.
Recent trends in the out-of-home of children in Australia (1997)

The recent collection of out-of-home care data by the Australian Institute of Health and Welfare has enabled an analysis of placement trends for the three year period 1993-1996. Significant findings include a sharp increase in overall numbers of children placed into care and a continuing decline in the use of residential/group care. The data are considered in the context of longer term placement trends and some implications for service delivery are discussed.
Inhibin Interferes with Activin Signaling at the Level of the Activin Receptor Complex in Chinese Hamster Ovary Cells<sup>1</sup> (1997)

To gain more insight in the mechanism of action of inhibin, we studied the effect of inhibin on activin signaling in Chinese hamster ovary cells. Inhibin specifically counteracted activin-induced expression of a plasminogen activator inhibitor 1 promoter element (3TP) and of the junB gene, but was ineffective when the responses were induced by transforming growth factor-beta. This indicates that inhibin acts only on the activin-specific part of these signaling cascades. Using a constitutively active activin type IB receptor we determined whether inhibin acted at the level of the activin-receptor complex or downstream of it. The mutant activin receptor stimulated the expression of the 3TP promoter in the absence of activin. This stimulation was insensitive to inhibin, indicating that inhibin acts exclusively at or upstream of this activin type I receptor. In addition, competition studies using labeled activin showed that inhibin displaced activin from the activin type II receptors, especially from the activin type IIB receptor, but not from the type I receptors. In conclusion, these data show that in Chinese hamster ovary cells inhibin acts directly at the activin receptor complex, most likely through displacement of activin from the activin type II receptor.
Low-power optical bistability and multistability in a self-electro-optic silicon interferometer (1985)

A novel hybrid semiconductor device is presented which shows optical bistability and a clear optical multistability at milliwatt input powers. Experimentally, a silicon etalon is used as a Fabry-Perot interferometer where an electric field is additionally applied by using integrated electrical contacts. At 1.06 μm the device exhibits the combined properties of a photodetector and an optical modulator. Due to this self-electro-optic effect a large optical nonlinearity is achieved which is theoretically attributed to electrical and optical heating of the sample.
Reaction of 1,2,4-triazines with N-phenylmaleimide. (1987)

Diels-Alder addition of N-phenylmaleimide (2) to 1, 2, 4-triazines (1) occurred across carbons 3 and 6 of the triazines to give 1 : 1 adducts. After losing nitrogen, they gave dihydropyridines, which underwent a variety of further reactions. Pyridine derivatives (3) and bis-adducts (4) containing a 2-azabicyclo [2.2.2] oct-2-ene residue were obtained.
Predicting positive and negative links in online social networks (2010)

We study online social networks in which relationships can be either positive (indicating relations such as friendship) or negative (indicating relations such as opposition or antagonism). Such a mix of positive and negative links arise in a variety of online settings; we study datasets from Epinions, Slashdot and Wikipedia. We find that the signs of links in the underlying social networks can be predicted with high accuracy, using models that generalize across this diverse range of sites. These models provide insight into some of the fundamental principles that drive the formation of signed links in networks, shedding light on theories of balance and status from social psychology; they also suggest social computing applications by which the attitude of one user toward another can be estimated from evidence provided by their relationships with other members of the surrounding social network.
Pilot-Assisted Decision-Aided Maximum-Likelihood Phase Estimation in Coherent Optical Phase-Modulated Systems With Nonlinear Phase Noise (2010)

<para xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> We proposed pilot-assisted (PA) decision-aided maximum-likelihood (DA ML) phase estimation for coherent optical <formula formulatype="inline"><tex Notation="TeX">$M$</tex></formula>-ary phase-shift-keying systems. In a nonlinear-phase-noise channel with laser phase noise, the required launched power at bit-error-rate (BER) <formula formulatype="inline"><tex Notation="TeX">$=10 ^{-4}$</tex> </formula> using the proposed PA DA ML algorithm without differential encoding (DE) can be reduced by about 0.7 dB in quadrature phase-shift keying and eight-phase-shift keying systems, and about 0.4 dB in 16-quadrature amplitude modulation format, compared to their DE counterparts using DA ML. </para>
Intranets vs. Lotus Notes (1996)

This text is intended for people who are looking for options beyond NetWare or NT networks, and who want the functionality of Lotus Notes without the training, maintenance and management usually associated with it. It explains how to build an Intranet, discusses the software needed, and how to use your Intranet locally to connect to a WAN. The comparisons to Lotus Notes provide a frame of reference. The book provides instruction on how to build a groupware system with a variety TCP/IP programs accessible via the Internet, and provides details on how to use the appropriate Internet programs for groupwork purposes.
Antimalarial compounds isolated from plants used in traditional medicine (2009)

Abstract Objectives This review covers the compounds with antiplasmodial activity isolated from plants published from 2005 to the end of 2008, organized according to their phytochemical classes. Details are given for substances with IC50 values ≤ 11 μM. Key findings Malaria is a major parasitic disease in many tropical and subtropical regions and is responsible for more than 1 million deaths each year in Africa. The rapid spread of resistance encourages the search for new active compounds. Nature and particularly plants used in traditional medicine are a potential source of new antimalarial drugs as they contain molecules with a great variety of structures and pharmacological activities. Summary A large number of antimalarial compounds with a wide variety of structures have been isolated from plants and can play a role in the development of new antimalarial drugs. Ethnopharmacological approaches appear to be a promising way to find plant metabolites that could be used as templates for designing new derivatives with improved properties.
Effects of Epidermal Growth Factor on Fibroblast Migration through Biomimetic Hydrogels (2003)

We have previously reported on the development and use of synthetic hydrogel extracellular matrix (ECM) analogues that can be used to study the mechanisms of migration. These biomimetic hydrogels consist of bioinert poly(ethylene glycol) diacrylate derivatives with proteolytically degradable peptide sequences included in the backbone of the polymer and adhesion peptide sequences grafted into the network. Cells adhere to the hydrogel via interaction between the grafted adhesion ligands and receptors on the cell surface. The cells migrate through the three-dimensional system by secreting the appropriate proteolytic enzymes, which are involved in cell migration and are targeted to the peptide sequences incorporated in the backbone of the polymer. It was observed that cell migration has a biphasic dependence on adhesion ligand concentration, with optimal migration at intermediate ligand levels. In this study, we demonstrate that we can covalently attach epidermal growth factor (EGF) to PEG and graft them into the hydrogels. It was observed that EGF when tethered maintained mitogenic activity. It was also observed that fibroblast migration significantly increased in the presence of the grafted EGF through the collagenase-sensitive hydrogels. In addition, the increase in migration was found to be independent from the proliferative response of the cells. These synthetic ECM analogues allow one to systematically control identities and concentrations of biomolecules and are useful tools to study mechanisms of cell migration.
Bismuth Nanoparticle Decorating Graphite Felt as a High-Performance Electrode for an All-Vanadium Redox Flow Battery (2013)

Employing electrolytes containing Bi(3+), bismuth nanoparticles are synchronously electrodeposited onto the surface of a graphite felt electrode during operation of an all-vanadium redox flow battery (VRFB). The influence of the Bi nanoparticles on the electrochemical performance of the VRFB is thoroughly investigated. It is confirmed that Bi is only present at the negative electrode and facilitates the redox reaction between V(II) and V(III). However, the Bi nanoparticles significantly improve the electrochemical performance of VRFB cells by enhancing the kinetics of the sluggish V(II)/V(III) redox reaction, especially under high power operation. The energy efficiency is increased by 11% at high current density (150 mA·cm(-2)) owing to faster charge transfer as compared with one without Bi. The results suggest that using Bi nanoparticles in place of noble metals offers great promise as high-performance electrodes for VRFB application.
3D deformation field throughout the interior of materials. (2013)

This report contains the one-year feasibility study for our three-year LDRD proposal that is aimed to develop an experimental technique to measure the 3D deformation fields inside a material body. In this feasibility study, we first apply Digital Volume Correlation (DVC) algorithm to pre-existing in-situ Xray Computed Tomography (XCT) image sets with pure rigid body translation. The calculated displacement field has very large random errors and low precision that are unacceptable. Then we enhance these tomography images by setting threshold of the intensity of each slice. DVC algorithm is able to obtain accurate deformation fields from these enhanced image sets and the deformation fields are consistent with the global mechanical loading that is applied to the specimen. Through this study, we prove that the internal markers inside the pre-existing tomography images of aluminum alloy can be enhanced and are suitable for DVC to calculate the deformation field throughout the material body.
Identification and antitumor activity of a novel inhibitor of the NIMA-related kinase NEK6 (2018)

The NIMA (never in mitosis, gene A)-related kinase-6 (NEK6), which is implicated in cell cycle control and plays significant roles in tumorigenesis, is an attractive target for the development of novel anti-cancer drugs. Here we describe the discovery of a potent ATP site-directed inhibitor of NEK6 identified by virtual screening, adopting both structure- and ligand-based techniques. Using a homology-built model of NEK6 as well as the pharmacophoric features of known NEK6 inhibitors we identified novel binding scaffolds. Twenty-five compounds from the top ranking hits were subjected to in vitro kinase assays. The best compound, i.e. compound 8 ((5Z)-2-hydroxy-4-methyl-6-oxo-5-[(5-phenylfuran-2-yl)methylidene]-5,6-dihydropyridine-3-carbonitrile), was able to inhibit NEK6 with low micromolar IC<sub>50</sub> value, also displaying antiproliferative activity against a panel of human cancer cell lines. Our results suggest that the identified inhibitor can be used as lead candidate for the development of novel anti-cancer agents, thus opening the possibility of new therapeutic strategies.
Primate antibodies to components of the human immune system (1994)

The feasibility to raise nonhuman primate antibodies against selected components of the human immune system was tested. The immunogens were whole cells (human T lymphocytes) or purified, recombinant human proteins (cytokines: TNF alpha or GM-CSF; soluble forms of cell surface antigens: sCD4 or sCD25). Significant immunizations, yielding functionally relevant antibodies, were readily achieved in rhesus monkeys, but, not surprisingly, may be less frequent in chimpanzees. The results suggest a general strategy for production of therapeutically useful MAB.
Differential gene expression analysis of strawberry cultivars that differ in fruit‐firmness (2003)

Firmness is an important selection criterium in the breeding of fruit, including strawberry ( Fragaria × ananassa Duch.). Clear differences in fruit‐firmness are observed between cultivars. In order to identify candidate genes which might be associated with such textural differences, gene expression levels were compared for a soft and a firm cultivar (cv. Gorella and cv. Holiday, respectively). DNA‐microarrays representing 1701 strawberry cDNAs were used for simultaneous hybridization of two RNA populations derived from red ripe fruit of both cultivars. In total 61 clones (3.6% of the total cDNAs on the arrays) displayed differential expression, including 10 clones (8 different ones) which showed homology to cell wall related genes in the public databases. The results from the microarray experiments were further confirmed by RNA gel blots, which were also used to examine gene expression in a third cultivar, Elsanta, showing an intermediate texture phenotype (offspring of a cross between Gorella and Holiday). Interestingly, two genes encoding proteins catalysing successive reactions in lignin metabolism (cinnamoyl CoA reductase and cinnamyl alcohol dehydrogenase) showed the highest difference in expression level.
Autocrine and paracrine growth control by granulocyte-monocyte colony- stimulating factor of acute lymphoblastic leukemia cells (1993)

Blast colony assays were performed on freshly obtained bone marrow samples from 19 newly diagnosed or relapsed children with acute lymphoblastic leukemia (ALL) of B lineage to determine the effect of added granulocyte-monocyte colony-stimulating factor (GM-CSF). Of the 19 marrow samples tested, 7 responded to GM-CSF with a mean increase in ALL blast colonies of 346%. Blast cells from one of the responders chosen for flow cytometric study showed expression of GM-CSF receptors on 38% of cells. These findings prompted us to establish five ALL cell lines of diverse phenotypes to examine the expression of GM-CSF and GM- CSF receptor genes in human leukemia, and to determine the role of GM- CSF in autocrine and paracrine growth control of ALL cells. One line, termed G2, manifested a GM-CSF-mediated autocrine pattern of cell growth with the following features: G2 blast colony growth in a serum- free system without added growth factor was density dependent; exogenous GM-CSF augmented G2 colony formation when the cells were seeded at low density; G2 cells constitutively expressed mRNA for GM- CSF and GM-CSF receptor; G2 cells also produced and secreted measurable amounts of GM-CSF into cell culture supernatant; and, monoclonal anti- GM-CSF antibodies abolished G2 colony growth when added to cultures with cells seeded at low density without growth factors. Of the other four ALL cell lines, three expressed mRNA for GM-CSF receptor and responded in vitro to added GM-CSF with increased blast colony growth; however, none of these four cell lines expressed mRNA for constitutive production of GM-CSF. A fifth ALL cell line lacked receptors for GM-CSF and did not respond in clonogenic assays to added GM-CSF. Thus, a bioregulator of normal hematopoiesis plays a central role in autocrine growth control of G2 ALL cells, and an important paracrine growth- promoting role in three of four other ALL cell lines.
Take Pride in America in Southern Nevada: Quarterly Progress Report, Period Ending March 31, 2006 (2006)

• A community-based roundtable met three times this quarter to suggest goals and objectives for the anti-litter strategic plan.\n• Project Manager Doug Joslin is representing the public lands on a Clark County Recycling Advisory Committee, which met twice this quarter.\n• Four public service announcements were produced by UNLV students, with one selected by the team for use in the Southern Nevada messaging campaign.\n• The Clint Eastwood Take Pride in America PSA aired 74 times in January 2006 and was viewed by 116,802 people.\n• The interagency team has begun arrangements for paper recycling in partnership with the Abitibi-Consolidated Paper Retriever program.\n• A draft of the anti-litter and dumping messaging campaign will be presented to the team on April 12.\n• Two clean-ups were completed this quarter at Lake Mead National Recreation Area.\n• A website for dumpster and roll-off requests was completed in March 2006.