gebhart/patrepeval huggingface dataset:
A new continuous epitope of hepatitis A virus (1998)

A new continuous epitope of hepatitis A virus (HAV) was defined in the VP3 protein. Convalescent sera recognised the synthetic peptide 3110-3121 (FWRGDLVFDFQV). The replacement of the arginine, glycine, or aspartic acid at positions 112, 113, or 114, respectively by other amino acids induced the loss of synthetic peptide recognition by human convalescent sera, thereby confirming the presence of an epitope in the original VP3(110-121) sequence. Shorter VP3 peptides such as VP3(110-119). VP3(110-117), and VP3(110-116) and a tandem repeat of VP3(111-116) failed to react with convalescent sera, indicating the importance of the entire peptide in the epitope structure. The maximum inhibition of human convalescent binding to HAV by the VP3(110-121) peptide was around 60%, and 50% inhibition was achieved at a peptide concentration of 2.3-2.4 micrograms/ml. Antibodies generated by this peptide bound to intact HAV and neutralised its infectivity. Antipeptide antibodies inhibited convalescent serum binding to HAV. Monoclonal antibodies H7C27 and MAK-4E7 inhibited completely binding of the antipeptide antibodies to HAV.
Comparison of Five Different Filters for the Removal of Leukocytes from Red Cell Concentrates (1992)

The leukocyte depletion capacity and performance of 5 filters designed for filtration of red cell concentrates (RCC) were compared by counting leukocytes, measuring red cell volumes and by histological examination of the filters after use. To eliminate interdonor differences, 5 buffy-coat-poor RCC were pooled (in each of 10 experiments) and subsequently split up into the original bags. The RCC were passed over the Cellselect filter, a column filled with cellulose acetate, and over flat-bed polyester filters: the Cellselect Optima, the Pall RC 50, the Leukostop and the Sepacell R-500. The filtration was shortest with the Pall RC 50 (p less than 0.001 compared to the other 4 filters). Leukocyte removal was most effective with the cellulose acetate filter (p less than 0.01 compared to the other 4 filters) followed by the Cellselect Optima polyester filter (p less than 0.02 compared to the remaining 3 filters). Residual leukocytes did not exceed 50 x 10(6) for any brand of filter. Red cell recovery was similar for all 5 filters with mean values from 86.1 to 89.2%. The leukocyte numbers, counted in Türk's solution or in propidium iodide, gave comparable values in hemocytometers applying light microscopy or fluorescent microscopy, respectively. Histological examination showed that lymphocytes were mainly removed by trapping, whereas granulocytes showed a variable pattern: adhesion in presence of platelets or trapping.
HIPS - A Virtual Reality Hip Prosthesis Implantation Simulator (2018)

We present the first VR training simulator for hip replacement surgeries. We solved the main challenges of this task - high and stable forces during the milling process while simultaneously a very sensitive feedback is required - by using an industrial robot for the force output and the development of a novel massively parallel haptic rendering algorithm with support for material removal.
Data Security in Vehicular Communication Networks (2009)

This chapter contains sections titled: Introduction Challenges of Data Security in Vehicular Networks Network, Applications, and Adversarial Model Security Infrastructure Cryptographic Protocols Privacy Protection Mechanisms Implementation Aspects Outlookand Conclusions References
Switching Function Implementation based on Graph (2011)

본 논문에서는 그래프에 기초하여 유한체상의 스위칭함수를 도출하는 알고리즘과 이를 바탕으로 스위칭함수를 회로로 구현하는 방법을 제안하였다. 방향성그래프의 경로 수로부터 행렬방정식을 유도한 후에 이에 따른 스위칭 함수회로설계 알고리즘을 제안하였으며, 설계된 회로와 함께 방향성 그래프의 특성을 만족할 수 있게 노드들에 대한 코드를 할당하는 알고리즘을 제안하였다. 본 논문에서 제안한 알고리즘을 통해 설계한 스위칭함수회로는 기존의 방법에 비해 좀 더 최적화된 스위칭함수회로를 구현할 수 있었으며, 제안한 스위칭함수회로설계 알고리즘을 통해 임의의 자연수의 경로 수를 갖는 방향성 그래프에 대한 설계가 가능하였다. 또한, 입출력단자 수의 감소, 회로구성의 간략화, 연산속도의 향상과 비용감소 등의 이점이 있었다. This paper proposes the method of switching function implementation using switching function extraction based on graph over finite fields. After we deduce the matrix equation from path number of directional graph, we propose the switching function circuit algorithm, also we propose the code assignment algorithm for nodes which is satisfied the directional graph characteristics with designed circuits. We can implement more optimal switching function compare with former algorithm, also we can design the switching function circuit which have any natural number path through the proposed switching function circuit implementation algorithms. Also the proposed switching function implementation using graph theory over finite fields have decrement number of input-output, circuit construction simplification, increment arithmetic speed and decrement cost etc.
Cutting and Machining Energetic Materials with a Femtosecond Laser (2003)

Abstract A femtosecond (fs) laser has been used as a tool for solving many problems involving access, machining, disassembly, inspection and avoidance of undesirable hazardous waste streams in systems containing energetic materials. Because of the unique properties of the interaction of ultrashort laser pulses with matter, the femtosecond laser can be used to safely cut these energetic materials in a precise manner without creating an unacceptable waste stream. Many types of secondary high explosives (HE) and propellants have been cut with the laser for a variety of applications ranging from disassembly of aging conventional weapons (demilitarization), inspection of energetic components of aging systems to creating unique shapes of HE for purposes of initiation and detonation physics studies. Hundreds of samples of energetic materials have been cut with the fs laser without ignition and, in most cases, without changing the surface morphology of the cut surfaces. The laser has also been useful in cutting nonenergetic components in close proximity to energetic materials.
<i>Arabidopsis thaliana</i> natural variation in temperature-modulated immunity uncovers transcription factor UNE12 as a thermoresponsive regulator (2019)

Abstract Temperature impacts plant immunity and growth but how temperature intersects with endogenous pathways remains unclear. Here we uncover variation between Arabidopsis thaliana natural accessions in response to two non-stress temperatures (22°C and 16°C) affecting accumulation of the thermoresponsive stress hormone salicylic acid (SA) and plant growth. Analysis of differentially responding A. thaliana accessions shows that pre-existing SA provides a benefit in limiting bacterial pathogen infection at both temperatures. Several A. thaliana genotypes display a capacity to mitigate negative effects of high SA on growth, indicating within-species plasticity in SA - growth tradeoffs. An association study of temperature x SA variation, followed by physiological and immunity phenotyping of mutant and over-expression lines, identifies the transcription factor unfertilized embryo sac 12 ( UNE12 ) as a temperature-responsive SA immunity regulator. Here we reveal previously untapped diversity in plant responses to temperature and a way forward in understanding the genetic architecture of plant adaptation to changing environments.
Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from <i>Lactococcus</i> and <i>Lactobacillus</i> spp (1993)

A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The purity of the resulting plasmid DNA makes it suitable for subsequent molecular manipulations. The convenience of the technique makes this rapid mini-prep procedure suitable for routine plasmid isolation from lactic acid bacteria.
Specific appetite, energetic and metabolomics responses to fat overfeeding in resistant-to-bodyweight-gain constitutional thinness (2014)

CTs showed specific adaptation to fat overfeeding: overall increase in anorexigenic hormonal profile, enhanced post prandial GLP-1 and PYY and inverse to controls changes in urine metabolomics. Overfeeding revealed a paradoxical positive energy balance contemporary to a lack of bodyweight gain, suggesting yet unknown specific energy expenditure pathways in CTs.
Continuous ECG monitoring in the management of pre-hospital health emergencies (2003)

For the last five years, a system for the management and coordination of pre-hospital health emergency has been in regular use at the Emergency Coordination Centre of Heraklion, as part of HYGEIAnet, the Regional Health Information Network of Crete. Approximately 20,000 emergency episodes per year are currently being logged in the system. The need for better support of cardiac cases (about 20% of the total) prompted the extension of the system to include the continuous acquisition of 12-lead ECGs and their real-time transmission to the coordination centre for evaluation by an expert. The 12-lead ECG module has been developed and integrated in the existing system with special attention to the provision of an easy user interface so that the extra work required to the ambulance personnel is negligible. The overall system has been carefully tested and is now in daily use.
New Dosing Regimens for Amifostine: A Pilot Study to Compare the Relative Bioavailability of Oral and Subcutaneous Administration with Intravenous Infusion (2002)

A phase I clinical trial was conducted to assess thefeasibility of a more convenient and safe dosing regime for the cytoprotective drug amifostine. Two alternative routes of administration, oral and subcutaneous (s.q.), each with a dose of 500 mg, were compared to a 7.5-minute intravenous (i.v.) infusion, with a dose of 200 mg/m2, in normal, healthy volunteers (N = 12). Bioavailability of amifostine (parent drug) and its pharmacologically active metabolite, WR-1065, was evaluated by comparing the area under the concentration-time curve (AUC) derived from HPLC analysis of amifostine and both protein-free and protein-bound WR-1065 in all three routes of administration. Results showed that SQ (but not oral) administration of amifostine could provide a more effective dosing regimen, in terms of both a reasonable AUC for the bound form of WR-1065 and decreased toxicity, compared to i.v. delivery. These data suggest that the protein-bound form of WR-1065 plays an important role in contributing to the bioavailability of this clinically useful cytoprotective drug.
Tumor necrosis factor-alpha increases Mn-SOD expression: protection against oxidant injury (1991)

Antioxidant enzymes, including superoxide dismutase, are important for protecting the lung against O2 injury. Manganese superoxide dismutase (Mn-SOD) is a superoxide anion (O2-.) scavenger located in the mitochondria, a primary site of O2-. production during hyperoxia. We studied the effects of tumor necrosis factor (TNF-alpha), a macrophage-derived cytokine, on Mn-SOD expression in human pulmonary adenocarcinoma cells. TNF-alpha significantly increased Mn-SOD activity and mRNA in a dose-and time-dependent manner. Mn-SOD activity was increased 3-fold and mRNA 20-fold after a 48-h incubation with TNF-alpha (25 ng/ml). To examine the mechanism of this increase, cells were incubated for 48 h with TNF-alpha (25 ng/ml) with or without cycloheximide (10 microns) or actinomycin D (10 micrograms/ml). Actinomycin D blocked the induction of Mn-SOD mRNA by TNF-alpha, but cycloheximide did not. These findings suggest that the effect of TNF-alpha requires gene transcription but not synthesis of new protein intermediates. To test the hypothesis that increased Mn-SOD protects against oxidative injury, pulmonary adenocarcinoma cells were incubated in TNF-alpha (25 ng/ml) for 48 h and then exposed to paraquat (PQ+), an intracellular O2-. generator. Cells pretreated with TNF-alpha had significantly improved survival in PQ+ compared with controls. At the LD50 (6 microns) for control cells, 95% of TNF-alpha-treated cells survived, 85% at the LD75 (10 microns), and 77% at the LD90 (14 microns). Our results suggest that the induction of Mn-SOD by TNF-alpha in pulmonary adenocarcinoma cells is pretranslationally mediated and that increasing Mn-SOD activity with TNF-alpha confers protection against O2 radicals.
WR-2721 and high-dose cisplatin: an active combination in the treatment of metastatic melanoma. (1987)

Cisplatin, alone or in combination with other chemotherapeutic agents, is relatively inactive against metastatic melanoma. Prior trials have demonstrated partial response (PR) rates of less than 10% with cisplatin alone. WR-2721 is an organic thiophosphate compound, which in the animal model, selectively protects normal tissues against the toxicity of cisplatin chemotherapy. During the course of a phase I trial of WR-2721 and cisplatin, objective PRs were noted in patients with far advanced metastatic melanoma. These observations led us to perform a phase II trial of WR-2721 and cisplatin. Thirty-six patients received 128 courses of WR-2721 before cisplatin (60 to 150 mg/m2). All patients had progressive disease before treatment. Objective PRs were observed in 19 of 36 evaluable patients (53%). Three additional patients had minor responses (MRs). PRs occurred in 53% of patients with prior chemotherapy (ten of 19). Sites of responding metastases were subcutaneous disease (15 of 19 patients), lymph nodes (16 of 21 patients), lung (four of ten patients), and liver (eight of 17 patients). The median duration of response was 4 months, with a mean of 4.5 months (range, 1 to 8 months). Transient nephrotoxicity was observed in less than 5% of courses. In all cases, renal function returned to normal within 1 to 2 weeks. Hematologic toxicity was mild and infrequent. Nine patients developed peripheral neuropathy following a median cisplatin dose of 670 mg/m2. Twenty patients experienced mild clinical hearing loss. These data suggest that WR-2721 may potentiate the antitumor activity of cisplatin in metastatic melanoma.
Bacteriotherapy and probiotics’ role on oral health (2005)

Oral infections constitute some of the most common and costly forms of infections in humans. The concept of microbial ecological change as a mechanism for preventing dental disease is an important one while altered microbial ecology may lead to dental disease. New methods such as probiotic approaches (i.e. whole bacteria replacement therapy) to eliminate pathogenic members of the microbiota can be investigated. Bacteriotherapy is an alternative and promising way to combat infections by using harmless bacteria to displace pathogenic microorganisms. Probiotics are one of these new agents which are widely used for their therapeutic action. Limited research is available showing that some probiotic cultures may help dental improvement. Present paper focuses on possible oral benefits of probiotics.
RSSI-Based Indoor Localization and Tracking Using Sigma-Point Kalman Smoothers (2009)

Solutions for indoor tracking and localization have become more critical with recent advancement in context and location-aware technologies. The accuracy of explicit positioning sensors such as global positioning system (GPS) is often limited for indoor environments. In this paper, we evaluate the feasibility of building an indoor location tracking system that is cost effective for large scale deployments, can operate over existing Wi-Fi networks, and can provide flexibility to accommodate new sensor observations as they become available. This paper proposes a sigma-point Kalman smoother (SPKS)-based location and tracking algorithm as a superior alternative for indoor positioning. The proposed SPKS fuses a dynamic model of human walking with a number of low-cost sensor observations to track 2-D position and velocity. Available sensors include Wi-Fi received signal strength indication (RSSI), binary infra-red (IR) motion sensors, and binary foot-switches. Wi-Fi signal strength is measured using a receiver tag developed by Ekahau, Inc. The performance of the proposed algorithm is compared with a commercially available positioning engine, also developed by Ekahau, Inc. The superior accuracy of our approach over a number of trials is demonstrated.
Dielectric and Electromechanical Properties of (Li, Na)NbO <sub>3</sub> Ceramics (1977)

Permittivity, dielectric loss tangent, radial coupling coefficient, and radial frequency constant have been measured as a function of composition for pressure‐sintered Li. r Na 1‐x NbO 3 in the range 0.015≤x≤0.15. An anomaly in each of these properties when plotted as a function of composition is associated with a structural transition between 2 ferroelectric phases; this transition lies near room temperature for compositions where x∼0.12. Compositions near this phase boundary exhibit room‐temperature properties which are of interest for high‐frequency filter applications.
Assessing Two Dimensions of Interpersonal Trust: Other-Focused Trust and Propensity to Trust (2021)

One's propensity to trust others and others' trustworthiness are two important aspects of interpersonal trust. Both theory and research suggest that it is possible to distinguish between an individual's propensity to trust (one's "trustingness" or the extent to which one feels able to trust others) and their other-focused trust (the extent to which one feels that others are worthy of our trust). However, there is as yet no measure that distinguishes between these two components of trust. In three studies, we examined the psychometrics of a proposed two-dimensional measure of trust that encompasses propensity to trust and other-focused trust components. To test discriminant validity, we also administered measures of personality, personal self-esteem, social capital, propensity to like people, perceived social support, as well as general and personal beliefs in a just world. Factor analyses supported the proposed two-factor model for the new trust measure. Further analyses supported the difference between these measures.
Therapeutic Strategies To Prevent Neurodegeneration And Promote Regeneration In Multiple Sclerosis (2005)

Multiple sclerosis (MS) is an immune-mediated demyelinating and degenerative disease of the central nervous system (CNS), with lesions predominantly occurring in the CNS white matter. The current treatment for MS relies on therapies that primarily target the peripheral immune response. However, it is clear that these strategies alone are insufficient for treating the chronic progressive disability that is the ultimate outcome of the disease. Axonal degeneration may be the primary determinant of fixed neurological deficits in MS. Here, we will discuss the contribution of axonal damage to MS pathogenesis, and potential cellular and molecular targets in the prevention of neurodegeneration. In addition, we will discuss potential molecular approaches to promote repair of CNS components in multiple sclerosis.
Vacuum inductive store/pulse compression experiments on a high power accelerator using plasma opening switches (1983)

The first results of experiments using plasma opening switches for inductive energy storage are described. The switch has been shown to conduct up to 200-kA current for ∼50 ns then to open in &lt;10 ns, transferring the current to an electron beam load. Inductive energy storage, pulse compression, and power multiplication are demonstrated. A simple model explaining the switch operation is presented.
MR angiography of congenital heart disease: Value of segmented two‐dimensional inflow technique and maximum‐intensity‐projection display (1994)

Magnetic resonance (MR) angiography of the cardiovascular system was evaluated in 41 patients with congenital heart disease by using a two-dimensional (2D) inflow technique based on a magnetization-prepared gradient-echo pulse sequence with segmented k-space data acquisition and electrocardiographic gating at 0.5 T. Inversion and saturation prepulses were used to suppress stationary tissue and enhance intravascular signal. Presaturation slabs were applied where certain vascular structures had to be suppressed. Sequence parameters were optimized by evaluating signal intensity and contrast characteristics for various flip angles and inversion and saturation delay times. The heart and intrathoracic vasculature were encompassed with 40-50 overlapping sections. Both 2D angiograms and maximum-intensity-projection images were evaluated. Combining data sets acquired in the sagittal and transverse orientations provided the most satisfactory information about the pulmonary arteries. The highest signal-to-noise ratios were obtained with a flip angle of 65 degrees and short prepulse delay times. Two-dimensional MR angiography can provide useful diagnostic information but requires a thorough understanding of in-plane and hemodynamically induced signal intensity changes.
Fragment analysis in small molecule discovery. (2002)

Cheminformatics is playing an ever-increasing role in small molecule drug discovery. The widespread use of high-throughput screening (HTS) and combinatorial chemistry techniques has led to the generation of large amounts of pharmacological data which, in turn, has catalyzed the development of computational methods designed to reduce the time and cost in identifying molecules suitable for pharmaceutical development. This review focuses on recent advances in the field of substructure analysis, an increasingly popular data mining technique with applications at many levels of the discovery process, including HTS, compound library design, virtual screening and the prediction of biological activity.
Resorcinol Crystallization from the Melt: A New Ambient Phase and New “Riddles” (2016)

Structures of the α and β phases of resorcinol, a major commodity chemical in the pharmaceutical, agrichemical, and polymer industries, were the first polymorphic pair of molecular crystals solved by X-ray analysis. It was recently stated that "no additional phases can be found under atmospheric conditions" (Druzbicki, K. et al. J. Phys. Chem. B 2015, 119, 1681). Herein is described the growth and structure of a new ambient pressure phase, ε, through a combination of optical and X-ray crystallography and by computational crystal structure prediction algorithms. α-Resorcinol has long been a model for mechanistic crystal growth studies from both solution and vapor because prisms extended along the polar axis grow much faster in one direction than in the opposite direction. Research has focused on identifying the absolute sense of the fast direction-the so-called "resorcinol riddle"-with the aim of identifying how solvent controls crystal growth. Here, the growth velocity dissymmetry in the melt is analyzed for the β phase. The ε phase only grows from the melt, concomitant with the β phase, as polycrystalline, radially growing spherulites. If the radii are polar, then the sense of the polar axis is an essential feature of the form. Here, this determination is made for spherulites of β resorcinol (ε, point symmetry 222, does not have a polar axis) with additives that stereoselectively modify growth velocities. Both β and ε have the additional feature that individual radial lamellae may adopt helicoidal morphologies. We correlate the appearance of twisting in β and ε with the symmetry of twist-inducing additives.
Nitriles in Organic Synthesis: Synthesis of new pyridazine, pyridine and pyrazolo[3,4‐b]pyridine derivatives (1989)

Abstract The reaction of diethyl monoiminomalonate with benzoylacetonitrile affords the ethyl 3‐amino‐4‐cyanopentanoate derivative 1 a . Compound 1 a condensed with salicylaldehyde to yield the coumarin 5 and coupled with arenediazonium salts to yield the arenehydrazones 6a – c . Compounds 6a , b could be converted into the aminopyridazinium carboxylate 8a , b on treatment with aqueous sodium hydroxide. The reaction of 6a – c with hydrazine and phenyl hydrazine is reported.