gebhart/patrepeval huggingface dataset:
Lipase dissolution and stabilization in ether-functionalized ionic liquids (2009)

Ionic liquids (ILs) with various structures and properties have been extensively investigated in many biocatalytic reactions and processes. However, although hydrophobic ILs tend to stabilize the insoluble (suspended) enzymes, they usually have low hydrogen-bonding basicity with solutes, which limits the solubility of many substrates (such as D-glucose, ascorbic acid, and cellulose). In contrast, hydrophilic ILs (such as those based on chloride, acetate and dicyanamide) are able to dissolve many of these substances that are not quite soluble in common organic solvents. Unfortunately, enzymes are not always active in these hydrophilic media due to strong interactions (such as H-bonding) between proteins and ILs. To resolve this dilemma, we recently synthesized new acetate-based ILs carrying a long alkyloxyalkyl chain in their cations, and found that these ether-functionalized solvents are lipase-compatible and can dissolve considerable amounts of D-glucose and cellulose (Green Chem., 2008, 10, 696). In this study, we further observed that these ILs could dissolve high concentrations of lipase B from Candida antarctica (CALB) (> 5 mg/mL at 50 °C), as well as other substrates including amino acids and betulinic acid. Therefore, these novel media offer new opportunities for carrying out homogeneous enzymatic reactions, which is practically important for large substrate molecules. In this article, we further confirmed the lipase compatibility of these ILs through the transesterification between ethyl butyrate and 1-butanol. The second derivative infrared spectra of CALB suggest the conservation of secondary structures of proteins in these ILs. We further investigated these ether-functionalized ILs in two important biocatalytic reactions: enzymatic synthesis of methyl-phthalate of betulinic acid, and CALB-catalyzed synthesis of D-glucose fatty acid esters. These substrates are not very soluble in conventional organic solvents, but very soluble in ILs, which improved the catalytic efficiency of these reactions. Moderate to high conversions were achieved in both reactions.
Indium Fluoroalkoxide Compounds (1999)

Reactions of indium amide complexes with fluorinated alcohols give indium fluoroalkoxide complexes. In[N-t-Bu(SiMe(3))](3) reacts with 3 equiv of (CF(3))Me(2)COH to give the homoleptic alkoxide dimer [In{&mgr;-OCMe(2)(CF(3))}{OCMe(2)(CF(3))}(2)](2), but reactions involving the more acidic alcohols (CF(3))(2)MeCOH and (CF(3))(2)CHOH yield products containing t-BuNH(2), which is derived from the amide ligands. Thus, In[N-t-Bu(SiMe(3))](3) reacts with (CF(3))(2)MeCOH to give In[OCMe(CF(3))(2)](3)(H(2)N-t-Bu) and with (CF(3))(2)CHOH to yield In[OCH(CF(3))(2)](3)(H(2)N-t-Bu)(3) and [H(3)N-t-Bu][In{OCH(CF(3))(2)}(4)(H(2)N-t-Bu)]. Reactions of (CF(3))(2)MeCOH and (CF(3))(2)CHOH with In(tmp)(3) (tmp = the anion derived from 2,2,6,6-tetramethylpiperidine) and In(NEt(2))(3) are less complicated. In(tmp)(3) reacts with 3 equiv of (CF(3))(2)CHOH to give In[OCH(CF(3))(2)](3)(Htmp) and with 4 equiv of (CF(3))(2)CHOH or (CF(3))(2)MeCOH to yield the salt compounds [H(2)tmp][In{OCR(CF(3))(2)}(4)] (R = H, Me). Trigonal bipyramidal [H(2)NEt(2)][In{OCH(CF(3))(2)}(4)(HNEt(2))] and octahedral mer-In[OCMe(CF(3))(2)](3)(py)(3) are isolated from reactions involving In(NEt(2))(3). Crystal structure determinations of [In{&mgr;-OCMe(2)(CF(3))}{OCMe(2)(CF(3))}(2)](2), [H(3)N-t-Bu][In{OCH(CF(3))(2)}(4)(H(2)N-t-Bu)].EtOEt, In[OCH(CF(3))(2)](3)(Htmp), [H(2)tmp][In{OCMe(CF(3))(2)}(4)], In[OCMe(CF(3))(2)](3)(py)(3), and [H(2)NEt(2)][In{OCH(CF(3))(2)}(4)(HNEt(2))] were carried out.
The production of new potato varieties technological advances (1987)

The potato is one of the world's most important food crops. It produces more nutritious food from less land in a shorter time than any other major crop. Therefore advances in the production of new potato varieties will greatly contribute to the world's food supply. The book outlines strategies used in the breeding and testing of potato varieties. It considers prospects for varietal improvement using both traditional and new techniques, including genetic manipulation, tissue culture and protoplast fusion. Opportunities for breeding varieties suitable for propagation from true seed, of particular significance to the agricultural systems of developing countries, are also featured, together with an extensive and bibliography. The book is based on the proceedings of a joint meeting of EAPR and EUCARPIA at King's College, Cambridge, December 1985. It contains contributions from many leading authorities in potato breeding and variety assessment throughout the world, and will interest post-graduate students in plant breeding, genetics and molecular biology, together with professionals engaged in potato research and the application of molecular biology to agriculture.
Field Evaluation of an Improved Formulation of Gypchek (a Nuclear Polyhedrosis Virus Product) Against the Gypsy Moth (Lepidoptera: Lymantriidae) (1999)

Prior to 1994, Gypchek® (USDA Forest Service, Washington, DC), the gypsy moth nuclear multicapsid polyhedrosis virus (LdMNPV), was used in operational programs against gypsy moth Lymantria dispar (L.) dispersed in a standard molasses-lignosulfonate tank mix and applied twice at the rate of 5 × 1011 polyhedral inclusion bodies (PIB) per ha per application in a volume of 19 liters per ha. In 1995, we evaluated a commercially-produced carrier and operational options that would make Gypchek application more efficient and less costly without reducing efficacy. Specifically, the standard tank mix formulation and application procedure was compared against a premixed commercial carrier, Carrier 038 (Novo Nordisk, Franklinton, NC) applied by three different application options. Option 1 consisted of double applications of Gypchek at the rate of 5 × 1011 PIB per ha per application in volumes of 9.5 liters per ha. Option 2 was identical to Option 1 except that application volume was reduced to 4.8 liters per ha. Option 3 consisted of a single application of Gypchek dispersed in 9.5 liters per ha at the rate of 1 × 1012 PIB per ha. There was also a fifth treatment consisting of unsprayed control plots. All treatments were evaluated in replicated 4-ha forest plots in southwestern Virginia. Levels of LdNPV-induced mortality, of larvae collected live 6 to 11 days after treatment, among treatments receiving a double application were not significantly different at α = 0.05 from such mortality resulting from the single application of Gypchek in Carrier 038. However, both of the double-application treatment options (but not the single application option) of Gypchek dispersed in Carrier 038 had significantly higher levels (α = 0.05) of LdMNPV than the double-application treatment of Gypchek dispersed in the standard tank mix. Defoliation was significantly different among the four Gypchek treatments, but all were significantly lower than the controls. These results indicated that all three options using Carrier 038 provided a level of efficacy equal to or better than the standard tank mix formulation applied twice at 19 liters/ha. Economic assessment indicated that the single application option was more efficient and/or less expensive than the other options.
Silicon Nanowire Transistors with a Channel Width of 4 nm Fabricated by Atomic Force Microscope Nanolithography (2008)

The emergence of an ultrasensitive sensor technology based on silicon nanowires requires both the fabrication of nanoscale diameter wires and the integration with microelectronic processes. Here we demonstrate an atomic force microscopy lithography that enables the reproducible fabrication of complex single-crystalline silicon nanowire field-effect transistors with a high electrical performance. The nanowires have been carved from a silicon-on-insulator wafer by a combination of local oxidation processes with a force microscope and etching steps. We have fabricated and measured the electrical properties of a silicon nanowire transistor with a channel width of 4 nm. The flexibility of the nanofabrication process is illustrated by showing the electrical performance of two nanowire circuits with different geometries. The fabrication method is compatible with standard Si CMOS processing technologies and, therefore, can be used to develop a wide range of architectures and new microelectronic devices.
Intraocular lens haptic fracturing with the neodymium:YAG laser (2006)

Neodymium:YAG laser haptic fracturing can be a viable option to cut the fibrosed haptic before explanting any IOL. It was demonstrated in vitro that it is easier to fracture hydrophilic acrylic and PMMA haptics than hydrophobic acrylic and polyvinylidene fluoride haptics.
A structural model of ultra-microelectrodes for shear-force based scanning electrochemical microscopy (2018)

The incorporation of a shear-force (SF) feedback in scanning electrochemical microscopy (SECM) hardware has enabled topographically resolved electrochemical imaging of electroactive substrates. Despite the versatility of SECM-SF imaging, structural response of the ultra-microelectrode (UME) to various excitation inputs is poorly understood and predictive mathematical models for characterizing dynamic behavior, particularly at high operating frequencies (>100 kHz), are absent. In this article, we present a finite element model to characterize SF behavior by modeling the UME as a rigid cantilever with two distributed piezoelectric wafers (dither and receiver) and demonstrate the model’s ability to predict experimentally observed SF behavior. The obtained SF response under different dither-to-receiver distances for various UME geometries and loading conditions provides insight to the optimum placement of piezoelectric wafers on the UME for achieving a high SF amplitude at SF-sensitive frequencies. In addition, the variations in SF response under different dither-to-receiver orientations indicate the existence of a system transfer function that is dependent on the operating modes of the receiver. The agreement between simulated and experimental results suggests that the finite element model along with the experimental methodology can be extended to automated SF imaging using SECM hardware.
A Method for Measuring Activated Factor VIII in Plasma (1991)

A method is described which enables a quantitative measurement of the concentration of activated factor VIII (VIIIa) in plasma. Based on the ability of factor VIIIa to accelerate the activation of factor X by factor IXa, phospholipid and calcium ions, the course of factor X activation in time is measured using a chromogenic substrate. Free factor Xa is able to activate nonactivated factor VIII present in a plasma sample, which increases the factor X activation velocity, and thus disturbs the measurement of factor VIIIa. Furthermore, factor Xa was found to be inactivated by serine protease inhibitors from the plasma sample. By adding surplus chromogenic substrate these reactions of factor Xa are inhibited and at the same time the rate of substrate conversion is a measure of the amount of factor Xa present. Factor X activation and amidolysis of chromogenic substrate then take place simultaneously. It is shown that under proper conditions the factor X activation velocity is linearly proportional to the factor VIIIa concentration. This causes the optical density to increase as a parabolic function of time. The concentration of factor VIIIa can be obtained from the quadratic coefficient of the equation describing the parabola. The method is specific for factor VIIIa in that the extrinsic factor X activator is shown to have no influence on the measurement of factor VIIIa in thromboplastin activated plasma. We conclude that a sensitive and reliable method for assessing factor VIIIa concentrations in plasma has been developed on the basis of simultaneous inhibition and measurement of factor Xa by a high concentration of chromogenic substrate.
Design and Construction of a First-Generation High-Throughput Integrated Robotic Molecular Biology Platform for Bioenergy Applications (2011)

The molecular biological techniques for plasmid-based assembly and cloning of gene open reading frames are essential for elucidating the function of the proteins encoded by the genes. High-throughput integrated robotic molecular biology platforms that have the capacity to rapidly clone and express heterologous gene open reading frames in bacteria and yeast and to screen large numbers of expressed proteins for optimized function are an important technology for improving microbial strains for biofuel production. The process involves the production of full-length complementary DNA libraries as a source of plasmid-based clones to express the desired proteins in active form for determination of their functions. Proteins that were identified by high-throughput screening as having desired characteristics are overexpressed in microbes to enable them to perform functions that will allow more cost-effective and sustainable production of biofuels. Because the plasmid libraries are composed of several thousand unique genes, automation of the process is essential. This review describes the design and implementation of an automated integrated programmable robotic workcell capable of producing complementary DNA libraries, colony picking, isolating plasmid DNA, transforming yeast and bacteria, expressing protein, and performing appropriate functional assays. These operations will allow tailoring microbial strains to use renewable feedstocks for production of biofuels, bioderived chemicals, fertilizers, and other coproducts for profitable and sustainable biorefineries.
Syrian Hamster Female Protein: Analysis of Female Protein Primary Structure and Gene Expression (1985)

The concentration in plasma of the female protein (FP) of the golden Syrian hamster is regulated by sex steroids and by mediators of the acute-phase response to tissue injury or inflammation. A complementary DNA (cDNA) clone corresponding to FP was isolated from a hamster liver cDNA library and used to determine the nucleotide sequence and derived amino acid sequence of native FP. The primary sequence of FP is 69 percent identical to human serum amyloid P component and 50 percent identical to human C-reactive protein. Evidence showed that sex-limited and acute-phase control of the FP gene is pretranslational. The FP protein is thus a useful model for investigating dual regulation of expression of a single gene.
Using CORBA and Java for PBX management (2002)

Telephone switches are characteristically long-lived, evolving systems. We describe how a legacy two-tier system for telephone switch management was re-engineered as a three-tier Web-based application using CORBA and Java. In this solution the design-time advantages of portability and abstraction brought by an IDL information model are combined with the universality and ease of use of a Java GUI. Performance was shown to be largely unaffected by the addition of the extra tier, while CORBA was seen to bring unexpected new advantages in the area of scripting and rapid application development (RAD).
A VECTOR PERTURBATION APPROACH TO THE GENERALIZED AIRCRAFT SPARE PARTS GROUPING PROBLEM (2000)

: The Vector Perturbation Approach is introduced for addressing the generalized parts grouping problem, identifying part families for a general set of suppliers, not just a single supplier. This method is driven by the need for flexible and lean supply chain systems. A vector space model is used to represent a set of operation sequences as opposed to the traditional matrix and integer programming models in Group Technology. Using this approach we find that we are able to generate part groups from 90% of the available parts, in which all the operation sequences are preserved. This contrasts with only 66% of the available parts grouped using the traditional methods. Furthermore, a vector representation of operation sequences provides an intuitive means for discovering the natural structure of the part data. From these results we conclude that this technique can dramatically improve the effectiveness of the entire supply chain. INTRODUCTION A key challenge to military readiness is the a...
Transcription and Regulation of the Bidirectional Hydrogenase in the Cyanobacterium <i>Nostoc</i> sp. Strain PCC 7120 (2007)

The filamentous, heterocystous cyanobacterium Nostoc sp. strain PCC 7120 (Anabaena sp. strain PCC 7120) possesses an uptake hydrogenase and a bidirectional enzyme, the latter being capable of catalyzing both H2 production and evolution. The completely sequenced genome of Nostoc sp. strain PCC 7120 reveals that the five structural genes encoding the bidirectional hydrogenase (hoxEFUYH) are separated in two clusters at a distance of approximately 8.8 kb. The transcription of the hox genes was examined under nitrogen-fixing conditions, and the results demonstrate that the cluster containing hoxE and hoxF can be transcribed as one polycistronic unit together with the open reading frame alr0750. The second cluster, containing hoxU, hoxY, and hoxH, is transcribed together with alr0763 and alr0765, located between the hox genes. Moreover, alr0760 and alr0761 form an additional larger operon. Nevertheless, Northern blot hybridizations revealed a rather complex transcription pattern in which the different hox genes are expressed differently. Transcriptional start points (TSPs) were identified 66 and 57 bp upstream from the start codon of alr0750 and hoxU, respectively. The transcriptions of the two clusters containing the hox genes are both induced under anaerobic conditions concomitantly with the induction of a higher level of hydrogenase activity. An additional TSP, within the annotated alr0760, 244 bp downstream from the suggested translation start codon, was identified. Electrophoretic mobility shift assays with purified LexA from Nostoc sp. strain PCC 7120 demonstrated specific interactions between the transcriptional regulator and both hox promoter regions. However, when LexA from Synechocystis sp. strain PCC 6803 was used, the purified protein interacted only with the promoter region of the alr0750-hoxE-hoxF operon. A search of the whole Nostoc sp. strain PCC 7120 genome demonstrated the presence of 216 putative LexA binding sites in total, including recA and recF. This indicates that, in addition to the bidirectional hydrogenase gene, a number of other genes, including open reading frames connected to DNA replication, recombination, and repair, may be part of the LexA regulatory network in Nostoc sp. strain PCC 7120.
Mechanisms of Action of Bisphosphonates (2005)

This chapter contains sections titled: Introduction Structure of Bisphosphonates and Targeting to Bone Effects of Bisphosphonates on Bone Cells Direct Effects on Osteoclasts Effects on Osteoclast Precursors Effects on Osteoblasts and Osteocytes Inhibition of FPP Synthase by Nitrogen-Containing Bisphosphonates Inhibition of FPP Synthase Causes Loss of Prenylated Proteins in Osteoclasts Metabolism of Non-Nitrogen-Containing Bisphosphonates to ATP Analogues Other Potential Molecular Mechanisms of Action Summary References
Heteromultimers of DEG/ENaC subunits form H <sup>+</sup> -gated channels in mouse sensory neurons (2002)

Acidic extracellular solution activates transient H(+)-gated currents in dorsal root ganglion (DRG) neurons. The biophysical properties of three degenerin/epithelial sodium (DEG/ENaC) channel subunits (BNC1, ASIC, and DRASIC), and their expression in DRG, suggest that they might underlie these H(+)-gated currents and function as sensory transducers. However, it is uncertain which of these DEG/ENaC subunits generate the currents, and whether they function as homomultimers or heteromultimers. We found that the biophysical properties of transient H(+)-gated currents from medium to large mouse DRG neurons differed from BNC1, ASIC, or DRASIC expressed individually, but were reproduced by coexpression of the subunits together. To test the contribution of each subunit, we studied DRG from three strains of mice, each bearing a targeted disruption of BNC1, ASIC, or DRASIC. Deletion of any one subunit did not abolish H(+)-gated currents, but altered currents in a manner consistent with heteromultimerization of the two remaining subunits. These data indicate that combinations of two or more DEG/ENaC subunits coassemble as heteromultimers to generate transient H(+)-gated currents in mouse DRG neurons.
Channel Estimation for mmWave MIMO With Transmitter Hardware Impairments (2017)

This letter considers the problem of channel estimation for millimeter wave (mmWave) multiple-input multiple-output systems under a transmitter impairments model. Specifically, taking the transmitter hardware impairments into account, the performance of conventional pilots-based channel estimation scheme will be degraded due to the destroyed training pilots. By exploiting the sparsity of mmWave channel in the angular domain, a new channel estimation algorithm based on the Bayesian compressive sensing (BCS) and least square estimation (LSE) is proposed. First, the expectation maximization algorithm is presented to solve the BCS problem, and the refined measurement matrix and the support of the channel vector are obtained. Next, the channel gain coefficients are estimated by using the LSE. Simulation results show that the proposed algorithm can achieve better performance compared with the conventional BCS and orthogonal matching pursuit algorithm.
Internal sequence analysis of proteins eluted from polyacrylamide gels (1996)

We have developed an elution-digestion-sequencing (EDS) method, which yields the internal amino acid sequence of partially purified proteins. The overall yield for the method was greater than 60%. The method yielded peptide peaks that could be sequenced on HPLC for all tested proteins with masses from 45 to 200.10(3) and yielded internal amino acid sequence information when as little as 10 pmol of partially purified protein was used as the starting material. The EDS method was extremely reliable and gave sequence information for each of 25 proteins tested, including high-molecular-mass proteins (M(r) > 100.10(3)) that were difficult to sequence by other methods.
Mechanics of Textile and Laminated Composites: With applications to structural analysis (1996)

Basic equations of elasticity. Theory of anisotropic elastic media. Elastic properties of fiber reinforced composites. Elastic characterization of textile reinforced composites. Elastic characterization of layered anisotropic media. 3-D analysis of laminated structures. Analysis of textile composite structures. Appendices. References. Index.
Synthesis and Herbicidal Activities of Novel Substituted Acetophenone Oxime Esters of Pyrithiobac (2020)

Abstract A series of substituted acetophenone oxime esters of Pyrithiobac were synthesized as potential herbicides. The structures of the synthesized new compounds were confirmed by 1 H NMR, 13 C NMR, HRMS, and X‐ray single‐crystal diffraction (XRD). The pre‐emergence and post‐emergence herbicidal activity studies show that 4‐CF 3 ( 6 f ), 4‐NO 2 ( 6 i ), 4‐NH 2 ( 6 j ), 4‐F ( 6 l ), and 2,4‐2OCH 3 ( 6 s ) substituted acetophenone oxime esters of Pyrithiobac exhibit better pre‐emergence and post‐emergence herbicidal activities against monocotyledon and dicotyledon weeds than other synthesized new compounds. In pre‐emergence herbicidal activity study, 6 f , 6 i , 6 j , 6 l , and 6 s exhibit similar herbicidal activities against monocotyledon and dicotyledon weeds as compared to Staple (Pyrithiobac‐sodium). In post‐emergence herbicidal activity study, 6 f , 6 i , 6 j , 6 l , and 6 s exhibit similar herbicidal activities against dicotyledon weeds and better herbicidal activities against monocotyledon weeds as compared to Staple. Compounds 6 i and 6 l with high herbicidal activities and similar binding mode with acetolactate synthase as compared to Staple were further subjected to crop safety study. The results show that the safety of compound 6 l to cotton is as good as Staple and better than 6 i . The present work indicates that compound 6 l may serve as a new candidate for potential herbicides.