gebhart/patrepeval huggingface dataset:
Implementation aspects of large vocabulary recognition based on intraword and interword phonetic units (1990)

Most large vocabulary speech recognition systems essentially consist of a training algorithm and a recognition structure which is essentially a search for the best path through a rather large decoding network. Although the performance of the recognizer is crucially tied to the details of the training procedure, it is absolutely essential that the recognition structure be efficient in terms of computation and memory, and accurate in terms of actually determining the best path through the lattice, so that a wide range of training (sub-word unit creation) strategies can be efficiently evaluated in a reasonable time period. We have considered an architecture in which we incorporate several well known procedures (beam search, compiled network, etc.) with some new ideas (stacks of active network nodes, likelihood computation on demand, guided search, etc.) to implement a search procedure which maintains the accuracy of the full search but which can decode a single sentence in about one minute of computing time (about 20 times real time) on a vectorized, concurrent processor. The ways in which we have realized this significant computational reduction are described in this paper.
Acoustic metamaterial design and applications (2010)

The explosion of interest in metamaterials is due to the dramatically increased manipulation\nability over light as well as sound waves. This material research was stimulated by the\nopportunity to develop an artificial media with negative refractive index and the application in superlens which allows super-resolution imaging. High-resolution acoustic imaging techniques are the essential tools for nondestructive testing and medical screening. However, the spatial resolution of the conventional acoustic imaging methods is restricted by the incident wavelength\nof ultrasound. This is due to the quickly fading evanescent fields which carry the subwavelength\nfeatures of objects. By focusing the propagating wave and recovering the evanescent field, a flat lens with negative-index can potentially overcome the diffraction limit. We present the first experimental demonstration of focusing ultrasound waves through a flat acoustic metamaterial lens composed of a planar network of subwavelength Helmholtz resonators. We observed a tight focus of half-wavelength in width at 60.5 KHz by imaging a point source. This result is in\nexcellent agreement with the numerical simulation by transmission line model in which we\nderived the effective mass density and compressibility. This metamaterial lens also displays\nvariable focal length at different frequencies. Our experiment shows the promise of designing compact and light-weight ultrasound imaging elements.\nMoreover, the concept of metamaterial extends far beyond negative refraction, rather giving\nenormous choice of material parameters for different applications. One of the most interesting examples these years is the invisible cloak. Such a device is proposed to render the hidden object undetectable under the flow of light or sound, by guiding and controlling the wave path through an engineered space surrounding the object. However, the cloak designed by transformation optics usually calls for a highly anisotropic metamaterial, which make the experimental studies\nremain challenging. We present here the first practical realization of a low-loss and broadband acoustic cloak for underwater ultrasound. This metamaterial cloak is constructed with a network of acoustic circuit elements, namely serial inductors and shunt capacitors. Our experiment clearly shows that the acoustic cloak can effectively bend the ultrasound waves around the hidden object, with reduced scattering and shadow. Due to the non-resonant nature of the building\nelements, this low loss (~6dB/m) cylindrical cloak exhibits excellent invisibility over a broad\nfrequency range from 52 to 64 kHz in the measurements. The low visibility of the cloaked object\nfor underwater ultrasound shed a light on the fundamental understanding of manipulation,\nstorage and control of acoustic waves. Furthermore, our experimental study indicates that this design approach should be scalable to different acoustic frequencies and offers the possibility for a variety of devices based on coordinate transformation.
Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4 (2009)

Although understanding of the olfactory system has progressed at the level of downstream receptor signaling and the wiring of olfactory neurons, the system remains poorly understood at the molecular level of the receptors and their interaction with and recognition of odorant ligands. The structure and functional mechanisms of these receptors still remain a tantalizing enigma, because numerous previous attempts at the large-scale production of functional olfactory receptors (ORs) have not been successful to date. To investigate the elusive biochemistry and molecular mechanisms of olfaction, we have developed a mammalian expression system for the large-scale production and purification of a functional OR protein in milligram quantities. Here, we report the study of human OR17-4 (hOR17-4) purified from a HEK293S tetracycline-inducible system. Scale-up of production yield was achieved through suspension culture in a bioreactor, which enabled the preparation of >10 mg of monomeric hOR17-4 receptor after immunoaffinity and size exclusion chromatography, with expression yields reaching 3 mg/L of culture medium. Several key post-translational modifications were identified using MS, and CD spectroscopy showed the receptor to be approximately 50% alpha-helix, similar to other recently determined G protein-coupled receptor structures. Detergent-solubilized hOR17-4 specifically bound its known activating odorants lilial and floralozone in vitro, as measured by surface plasmon resonance. The hOR17-4 also recognized specific odorants in heterologous cells as determined by calcium ion mobilization. Our system is feasible for the production of large quantities of OR necessary for structural and functional analyses and research into OR biosensor devices.
The CitST two‐component system regulates the expression of the Mg‐citrate transporter in <i>Bacillus subtilis</i> (2000)

citS and citT genes encoding a new two-component system were identified in the 71 degrees region between the pel and citM loci on the Bacillus subtilis chromosome. citS- and citT-deficient strains were unable to grow on minimal plates including citrate as a sole carbon source. In addition, a strain deficient in citM, which encodes the secondary transporter of the Mg-citrate complex, exhibited the same phenotype on this medium. Northern blot analysis revealed that citM was polycistronically transcribed with the downstream yflN gene, and that CitS and CitT were necessary for transcription of the citM-yflN operon. Upon addition of 2 mM citrate to DSM, this operon was strongly induced after the middle of the exponential growth phase in the wild type, but not in the citST double null mutant. Moreover, the transcription of this operon was completely repressed in the presence of 1% glucose. We found a sequence exhibiting homology to a catabolite-responsive element (cre) in the citM promoter region. Glucose repression was lost in ccpA and citM-cre mutants. From the result of a citM-promoter deletion experiment, putative CitT target sequences were found to be located around two regions, from -62 to -74 and from -149 to -189, relative to the citM start point. Furthermore, DNase I footprinting assays revealed that these two CitT target regions extended maximally from -36 to -84 and from -168 to -194. From these findings, we concluded that the expression of citM is positively regulated by the CitST system and negatively regulated by CcpA.
Direct Involvement of the Small GTPase Rac in Activation of the Superoxide-producing NADPH Oxidase Nox1 (2006)

Activation of the non-phagocytic superoxide-producing NADPH oxidase Nox1, complexed with p22(phox) at the membrane, requires its regulatory soluble proteins Noxo1 and Noxa1. However, the role of the small GTPase Rac remained to be clarified. Here we show that Rac directly participates in Nox1 activation via interacting with Noxa1. Electropermeabilized HeLa cells, ectopically expressing Nox1, Noxo1, and Noxa1, produce superoxide in a GTP-dependent manner, which is abrogated by expression of a mutant Noxa1(R103E), defective in Rac binding. Superoxide production in Nox1-expressing HeLa and Caco-2 cells is decreased by depletion or sequestration of Rac; on the other hand, it is enhanced by expression of the constitutively active Rac1(Q61L), but not by that of a mutant Rac1 with the A27K substitution, deficient in binding to Noxa1. We also demonstrate that Nox1 activation requires membrane recruitment of Noxa1, which is normally mediated via Noxa1 binding to Noxo1, a protein tethered to the Nox1 partner p22(phox): the Noxa1-Noxo1 and Noxo1-p22(phox) interactions are both essential for Nox1 activity. Rac likely facilitates the membrane localization of Noxa1: although Noxa1(W436R), defective in Noxo1 binding, neither associates with the membrane nor activates Nox1, the effects of the W436R substitution are restored by expression of Rac1(Q61L). The Rac-Noxa1 interaction also serves at a step different from the Noxa1 localization, because the binding-defective Noxa1(R103E), albeit targeted to the membrane, does not support superoxide production by Nox1. Furthermore, a mutant Noxa1 carrying the substitution of Ala for Val-205 in the activation domain, which is expected to undergo a conformational change upon Rac binding, fully localizes to the membrane but fails to activate Nox1.
An analysis of field performance of cellular insulated filled telephone cable after a decade of service (1986)

Abstract : It is twelve years since cellular insulated filled telephone cable was introduced to American telephony systems. Despite early concerns regarding certain properties of expanded insulation, cellular insulated filled cables have proven to be a dependable cable design with optimal transmission properties, good reliability, and low maintenance. Shortly after the introduction of cellular insulated filled telephone cable, cables from early production runs were installed in various field test sites. Annual electrical measurements have been made and the electrical performance of these cables has been documented. Results of physical testing on samples recently recovered from the field test sites show the condition of cable materials after a decade of service.
Domain wall cloning in magnetic nanowires (2007)

We demonstrate cloning of magnetic domain wall solitons using a nanowire junction. The effect of junction geometry is assessed using several different structure designs to construct operating phase diagrams. A domain wall cloning element is then integrated with a magnetic domain wall NOT gate and feedback loop to unambiguously demonstrate domain wall duplication. Finally, we demonstrate four times digital amplification of a signal by siting cloning elements sequentially. This can be used for signal routing in nanowire circuits or providing readout amplification for interfacing magnetic nanostructures with macroscopic probes such as magneto-optical beams.
Stereoselective Anion Template Effects: Syntheses and Molecular Structures of Tetraphenyl [12]Mercuracarborand-4 Complexes of Halide Ions (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereoselective Anion Template Effects: Syntheses and Molecular Structures of Tetraphenyl [12]Mercuracarborand-4 Complexes of Halide IonsZhiping Zheng, Carolyn B. Knobler, and M. Frederick HawthorneCite this: J. Am. Chem. Soc. 1995, 117, 18, 5105–5113Publication Date (Print):May 1, 1995Publication History Published online1 May 2002Published inissue 1 May 1995https://doi.org/10.1021/ja00123a012RIGHTS & PERMISSIONSArticle Views233Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (4 MB) Get e-AlertsSupporting Info (3)»Supporting Information Supporting Information Get e-Alerts
Using the Congestion Quantifier Seven-Item Test to assess change in patient symptoms and their impact (2008)

Generally, nasal congestion is reported to be the most troublesome symptom of allergic rhinitis and has been shown to have negative effects on sleep, productivity, work, and school performance. A new patient-reported outcome (PRO) tool called the Congestion Quantifier Seven-Item Test (CQ7) was developed and shown to be a reliable and valid screening tool for nasal congestion. The purpose of this study was to assess the CQ7 as a tool for clinical use that can monitor changes in nasal congestion or determine if patients are showing meaningful improvement in symptoms. This study examined the responsiveness of the CQ7 to changes in nasal congestion and changes in relevant PRO to explore whether the CQ7 can serve as a monitoring tool and a valid screening tool for nasal congestion using latent growth curve models. Results showed that the CQ7 is sensitive to changes in patients' symptoms and their impact. Change in the CQ7 from baseline to day 15 was highly related to the change in symptom scores from baseline to day 15 (beta = -0.99); and regression coefficients for the relationships between the CQ7 and changes in the other PRO subscales also were very high (betas ranged from -0.79 to -0.95), lending evidence to support the use of the CQ7 as a monitoring tool. The large sizes of the coefficients suggest that the efficient, seven-item monitoring tool captures change in congestion very well. Analyses of responsiveness to change suggest that a three- to four-point improvement is meaningful to patients.
Outer Membrane Vesicle Production by <i>Escherichia coli</i> Is Independent of Membrane Instability (2006)

It has been long noted that gram-negative bacteria produce outer membrane vesicles, and recent data demonstrate that vesicles released by pathogenic strains can transmit virulence factors to host cells. However, the mechanism of vesicle release has remained undetermined. This genetic study addresses whether these structures are merely a result of membrane instability or are formed by a more directed process. To elucidate the regulatory mechanisms and physiological basis of vesiculation, we conducted a screen in Escherichia coli to identify gene disruptions that caused vesicle over- or underproduction. Only a few low-vesiculation mutants and no null mutants were recovered, suggesting that vesiculation may be a fundamental characteristic of gram-negative bacterial growth. Gene disruptions were identified that caused differences in vesicle production ranging from a 5-fold decrease to a 200-fold increase relative to wild-type levels. These disruptions included loci governing outer membrane components and peptidoglycan synthesis as well as the sigma(E) cell envelope stress response. Mutations causing vesicle overproduction did not result in upregulation of the ompC gene encoding a major outer membrane protein. Detergent sensitivity, leakiness, and growth characteristics of the novel vesiculation mutant strains did not correlate with vesiculation levels, demonstrating that vesicle production is not predictive of envelope instability.
Heterogeneity in a room-temperature ionic liquid: Persistent local environments and the red-edge effect (2006)

In this work, we investigate the slow dynamics of 1-butyl-3-methylimidazolium hexafluorophosphate, a very popular room-temperature ionic solvent. Our study predicts the existence of heterogeneity in the liquid and shows that this heterogeneity is the underlying microscopic cause for the recently reported "red-edge effect" (REE) observed in the study of fluorescence of the organic probe 2-amino-7-nitrofluorene. This theoretical work explains in microscopic terms the relation between REE and dynamic heterogeneity in a room-temperature ionic liquid (IL). The REE is typical of micellar or colloidal systems, which are characterized by microscopic environments that are structurally very different. In contrast, in the case of this room-temperature IL, the REE occurs because of the long period during which molecules are trapped in quasistatic local solvent cages. This trapping time, which is longer than the lifetime of the excited-state probe, together with the inability of the surroundings to adiabatically relax, induces a set of site-specific spectroscopic responses. Subensembles of fluorescent molecules associated with particular local environments absorb and emit at different frequencies. We describe in detail the absorption wavelength-dependent emission spectra of 2-amino-7-nitrofluorene and show that this dependence on lambda(ex) is characteristic of the IL and, as is to be expected, is absent in the case of a normal solvent such as methanol.
A bit-interleaved embedded hamming scheme to correct single-bit and multi-bit upsets for SRAM-based FPGAs (2014)

Single Event Upsets (SEUs) inadvertently change the configuration bits of Static-RAM (SRAM)-based Field Programmable Gate Arrays (FPGAs), leading to erroneous output until the error has been corrected. Scrubbing using an Error Correction Code (ECC) such as hamming is a popular method to correct such faults. However, current works either require a large external memory to store the ECCs or can at most correct only one error in a frame. This paper proposes a novel bit-interleaved embedded hamming scheme along with scrubbing, to correct single (SBUs) and multi-bit upsets (MBUs) in SRAM-based FPGAs. This scheme does not require an external memory to store the ECCs, as they are embedded within the configuration memory itself. Experiments conducted on various benchmarks show that the proposed scheme can handle multiple errors per frame very well, with an embedding efficiency of over 99.3%.
Music-Supported Training is More Efficient than Functional Motor Training for Recovery of Fine Motor Skills in Stroke Patients (2010)

MOTOR IMPAIRMENTS ARE COMMON AFTER STROKE but efficacious therapies for these dysfunctions are scarce. Extending an earlier study on the effects of music-supported training (MST), behavioral indices of motor function were obtained before and after a series of training sessions to assess whether this new treatment leads to improved motor functions. Furthermore, music-supported training was contrasted to functional motor training according to the principles of constraint-induced therapy (CIT). In addition to conventional physiotherapy, 32 stroke patients with moderately impaired motor function and no previous musical experience received 15 sessions of MST over a period of three weeks, using a manualized, step-bystep approach. A control group consisting of 15 patients received 15 sessions of CIT in addition to conventional physiotherapy. A third group of 30 patients received exclusively conventional physiotherapy and served as a control group for the other three groups. Fine as well as gross motor skills were trained by using either a MIDI-piano or electronic drum pads programmed to emit piano tones. Motor functions were assessed by an extensive test battery. MST yielded significant improvement in fine as well as gross motor skills with respect to speed, precision, and smoothness of movements. These improvements were greater than after CIT or conventional physiotherapy. In conclusion, with equal treatment intensity, MST leads to more pronounced improvements of motor functions after stroke than CIT.
An Unanswered Question About Mandatory Arbitration: Should a Mandatory Arbitration Clause Preclude the EEOC From Seeking Monetary Relief on an Employee's Behalf in a Title VII Case? (2000)

Introduction 282 I. Background 285 A. Gilmer and Mandatory Arbitration Under the FAA 285 B. Title VII Statutory Scheme and Remedies 288 II. Circuit Split on Scope of EEOC Remedies and Monetary Relief 292 A. Only Injunctive Relief 292 1. Second Circuit: EEOC v. Kidder, Peabody & Co 292 2. Fourth Circuit: EEOC v. Waffle House, Inc. 297 B. Both Monetary and Injunctive Relief 300 1. Sixth Circuit: EEOC v. Frank’s Nursery & Crafts, Inc. ...300 2. Sixth Circuit: EEOC v. Northwest Airlines 307 III. Analysis and Recommendations 310 A. Consensus Regarding Injunctive Relief 310 B. Only Injunctive Relief 311 C. Both Monetary and Injunctive Relief 316 Conclusion 322
Matrix Effects by Specific Buffer Components in the Analysis of Metabolites with Ion Trap Mobility Spectrometry (2008)

The impact of buffer conditions on the ion trap mobility spectrometer (ITMS) signal is investigated through a series of statistically driven design of experiments (DOEs). A growing number of new ion mobility spectrometry (IMS) and ITMS applications are being performed with a physiological sample matrix, which is vastly different from the particulate and vapor matrixes that have been traditionally analyzed. Currently, there have been no efforts to globally examine the possibility of matrix suppression or enhancement of the IMS signal by these various components within physiological matrixes. This investigation consists of an effort to gauge the effects of common physiological buffer components and concentrations on two analytes of interest for ITMS analysis: o-nitrophenol and ephedrine. We show that, among the factors investigated, for a specific analyte and instrumental detection mode (i.e., negative/positive) the solution pH, presence of a protein, the buffer identity, and buffer concentration should be considered as they will enhance or suppress the ITMS signal while factors such as surfactant and salt concentration may play less of a role in impacting detectable ITMS signal. These observations are supported through statistical analysis of the DOE-derived data set.