gebhart/patrepeval huggingface dataset:
Vacuolar H <sup>+</sup> -ATPase in ocular ciliary epithelium (1997)

The mechanisms controlling the production of aqueous humor and the regulation of intraocular pressure are poorly understood. Here, we provide evidence that a vacuolar H+-ATPase (V-ATPase) in the ocular ciliary epithelium is a key component of this process. In intracellular pH (pHi) measurements of isolated ciliary epithelium performed with 2',7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF), the selective V-ATPase inhibitor bafilomycin A1 slowed the recovery of pHi in response to acute intracellular acidification, demonstrating the presence of V-ATPase in the plasma membrane. In isolated rabbit ciliary body preparations examined under voltage-clamped conditions, bafilomycin A1 produced a concentration-dependent decrease in short-circuit current, and topical application of bafilomycin A1 reduced intraocular pressure in rabbits, indicating an essential role of the V-ATPase in ciliary epithelial ion transport. Immunocytochemistry utilizing antibodies specific for the B1 isoform of the V-ATPase 56-kDa subunit revealed localization of V-ATPase in both the plasma membrane and cytoplasm of the native ciliary epithelium in both rabbit and rat eye. The regional and subcellular distribution of V-ATPase in specific regions of the ciliary process was altered profoundly by isoproterenol and phorbol esters, suggesting that change in the intracellular distribution of the enzyme is a mechanism by which drugs, hormones, and neurotransmitters modify aqueous humor production.
The Pulmonary Surfactant: Control of Fluidity at the Air-Liquid Interface (1980)

The mammalian alveolus is stabilized by the presence of a specialized material, the pulmonary surfactant, which reduces the surface tension at the air-liquid interface. The disaturated lecithin, 1,2-dipalmitoyl-sn-phosphatidylcholine, is a major constituent of the surfactant. Unsaturated lecithins, other phospholipids, neutral lipid and protein are also present. The properties of compressed films of natural surfactant strongly resemble those of a monolayer of dipalmitoylphosphatidylcholine. However, below the transition temperature of 41°C, liposomes of dipalmitoylphosphatidylcholine are in the condensed gel state from which monolayers form very slowly. The mechanism by which dipalmitoylphosphatidylcholine is transferred from bilayers to monolayers has been investigated with a pulsating bubble technique. Natural surfactant reduces the surface tension of the pulsating bubble to 25–30 dynes/cm at maximal radius (0.55 mm) and to 0 dynes/cm at minimal radius (0.4 mm). Evidence is presented which strongly indicates that the proteins associated with natural surfactant are not required for the rate or the extent of the reduction of surface tension. Divalent cations appear to be important for surfactant behaviour. Using combinations of pure lipids, artificial surfactant preparations have been obtained which possess the basic physical and biological properties of natural surfactant.
Powertrain control verification benchmark (2014)

Industrial control systems are often hybrid systems that are required to satisfy strict performance requirements. Verifying designs against requirements is a difficult task, and there is a lack of suitable open benchmark models to assess, evaluate, and compare tools and techniques. Benchmark models can be valuable for the hybrid systems research community, as they can communicate the nature and complexity of the problems facing industrial practitioners. We present a collection of benchmark problems from the automotive powertrain control domain that are focused on verification for hybrid systems; the problems are intended to challenge the research community while maintaining a manageable scale. We present three models of a fuel control system, each with a unique level of complexity, along with representative requirements in signal temporal logic (STL). We provide results obtained by applying a state of the art analysis tool to these models, and finally, we discuss challenge problems for the research community.
Immune responses in newly developed short-lived SAM mice. III. Genetic control of defective helper T-cell activity in in vitro primary antibody response. (1989)

Immune activities of newly developed, short-lived SAM-P/1 mice declined sharply after a few months of age. As early as 2 months of age, the activity of T-helper (Th) cells ('Th2'-like) in the in vitro primary antibody response was profoundly impaired, in contrast to normal activity of Th cells ('Th1'-like) engaged in cell-mediated immune responses. Thus, young SAM-P/1 mice show a functional heterogeneity of Th cells. To determine how such a 'Th2' abnormality is inherited in SAM-P/1 mice, immune activities of their hybrids and backcrosses between MHC-identical, high responder B10.BR mice were statistically assessed. The distribution of responses did not support the Mendelian single-gene determination for low responsiveness. Moreover, involvement of a single gene which exhibits incomplete dominance was ruled out because of a continuous distribution pattern of antibody response in the F2 generation. Such an analysis strongly suggests that the impaired 'Th2'-like activity of SAM-P/1 mice is under control of two genes, based on the proportion of low responders in F2 hybrids (29 out of 267, 10.8%) and on calculation according to Wright's formula (n = 1.72). Further linkage analyses suggest that one of the genes is closely linked to albino coat-colour (c) locus on chromosome 7. The putative two genes are likely to control 'differentiation' or 'maturation' of Th2-like cells defectively, but the defect is not refractory, because in vivo-primed Th cells function in vitro as do those in ordinary strains of mice. Possible mechanisms and biological significance in relation to loss of immune activity with ageing are discussed.
Soluble factor(s) produced by adult bone marrow stroma inhibit in vitro proliferation and differentiation of fetal liver BFU-E by inducing apoptosis. (1997)

Hematopoiesis occurs in different organs during fetal development. Several studies suggest that the growth of hematopoietic progenitors at one stage of ontogenic maturation may not be supported by a microenvironment from a different ontogenic stage. To determine if human fetal liver (FL) clonogenic progenitors can develop in an adult bone marrow (ABM) microenvironment, we compared growth of BFU-E and CFU-GM from 7-14-wk-old FL, 11-20-wk-old fetal bone marrow (FBM), umbilical cord blood (UCB), or ABM in clonogenic medium with or without ABM stroma. In contrast to BFU-E from FBM, UCB, or ABM, soluble factor(s) produced by ABM stroma severely suppressed growth of 98% of FL BFU-E by inducing apoptosis of cells beyond early erythroblast stage. The nature of the soluble factor remains unknown, although we have evidence that it is heat labile with molecular mass < 10 kD. Antibody neutralization studies indicate that TGF-beta1, IL-1, TNF-alpha, macrophage inflammatory protein (MIP)-1alpha, or IFN-gamma are not responsible. The observation that FL progenitors may not be capable of differentiating when transferred to an ABM microenvironment may have important implications for FL transplantation into postnatal recipients. Further, this demonstrates that ontogenic stage-specific interactions between hematopoietic progenitors and their microenvironment are important for the normal development of hematopoiesis.
Sintering Theory and Fundamentals (2015)

Abstract Sintering is a thermal treatment process in which a powder or a porous material, already formed into the required shape, is converted into a useful article with the requisite microstructure. Sintering can be classified as solid-state, viscous, liquid-phase, and pressure-assisted (or pressure) sintering. This article provides information on the mechanisms and theoretical analysis of sintering and focuses on the types, mechanisms, process and microstructural variables, computer simulation, stages, and fundamentals of densification and grain growth of solid-state sintering and liquid-phase sintering. It describes the models for viscous sintering and the methods used in pressure-assisted sintering, namely, uniaxial hot pressing, hot isostatic pressing, sinter forging, and spark plasma sintering.
Hand-Held Holography (1967)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text G. W. Stroke, A. Funkhouser, C. Leonard, G. Indebetouw, and R. G. Zech, "Hand-Held Holography," J. Opt. Soc. Am. 57, 110-110 (1967) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Transistor level micro-placement and routing for two-dimensional digital VLSI cell synthesis (1999)

Abstract : The automated synthesis of mask geometry for VLSI leaf cells, referred to as the cell synthesis problem, is an important component of any structured custom integrated circuit design environment. Traditional approaches based on the classic functional cell style of Uehara & VanCleemput pose this problem as a straightforward one-dimensional graph optimization problem for which optimal solution methods are known. However, these approaches are only directly applicable to static CMOS circuits and they break down when faced with more exotic logic styles. Our methodology is centered around techniques for the efficient modeling and optimization of geometry sharing. Chains of diffusion-merged transistors are formed explicitly and their ordering optimized for area and global routing. In addition, more arbitrary merged structures are supported by allowing electrically compatible adjacent transistors to overlap during placement. The synthesis flow in TEMPO begins with a static transistor chain formation step. These chains are broken at the diffusion breaks and the resulting sub-chains passed to the placement step. During placement, an ordering is found for each chain and a location and orientation is assigned to each sub-chain. Different chain orderings affect the placement by changing the relative sizes of the sub-chains and their routing contribution. We conclude with a detailed routing step and an optional compaction step.
Low-temperature synthesis of multilayer graphene/amorphous carbon hybrid films and their potential application in solar cells (2012)

The effect of reaction temperature on the synthesis of graphitic thin film on nickel substrate was investigated in the range of 400°C to 1,000°C. Amorphous carbon (a-C) film was obtained at 400°C on nickel foils by chemical vapor deposition; hybrid films of multilayer graphene (MLG) and a-C were synthesized at a temperature of 600°C, while MLG was obtained at temperatures in excess of 800°C. Schottky-junction solar cell devices prepared using films produced at 400°C, 600°C, 800°C, and 1,000°C coupled with n-type Si demonstrate power conversion efficiencies of 0.003%, 0.256%, 0.391%, and 0.586%, respectively. A HNO3 treatment has further improved the efficiencies of the corresponding devices to 0.004%, 1.080%, 0.800%, and 0.820%, respectively. These films are promising materials for application in low-cost and simple carbon-based solar cells.
Power Semiconductor Drives (1984)

This textbook introduces students to the underlying principles of operation of power semiconductor drives. It explains every facet of application of power electronics to the control of electric motors in industrial drives. The book is primarily intended for B.E./B.Tech. students of Electrical Engineering/ Electrical and Electronics Engineering having courses in Electric Drives/ Power Semiconductor Drives. It will also be highly useful for M.E./M.Tech. students of these disciplines specializing in Power Electronics/Industrial Drives/Electric Drives.

The text is divided into eight chapters. The first two chapters cover the control of dc motors by using various kinds of converters. The third chapter focuses on dual converters and various braking techniques. Chopper control fed dc motors are discussed in the fourth chapter. The next three chapters are devoted to control methods for induction motors. The eighth chapter deals with the control of synchronous motor drives fed from VSI converters and cycloconverters.

Extensively illustrated, the book contains numerous solved examples throughout the text as well as a variety of chapterend questions to help in comprehending as well as in strengthening the grasp of the underlying concepts and principles. 
(Goodreads)
Effect of Oxygen Tension and Alginate Encapsulation on Restoration of the Differentiated Phenotype of Passaged Chondrocytes (2001)

The implantation of laboratory-grown tissue offers a valuable alternative approach to the treatment of cartilage defects. Procuring sufficient cell numbers for such tissue-engineered cartilage is a major problem since amplification of chondrocytes in culture typically leads to loss of normal cell phenotype yielding cartilage of inferior quality. In an effort to overcome this problem, we endeavored to regain the differentiated phenotype of chondrocytes after extensive proliferation in monolayer culture by modulating cell morphology and oxygen tension towards the in vivo state. Passaged cells were encapsulated in alginate hydrogel in an effort to regain the more rounded shape characteristic of differentiated chondrocytes. These cultures were exposed to reduced (5%-i.e., physiological), or control (20%) oxygen tensions. Both alginate encapsulation and reduced oxygen tension significantly upregulated collagen II and aggrecan core protein expression (differentiation markers). In fact, after 4 weeks in alginate at 5% oxygen, differentiated gene expression was comparable to primary chondrocytes. Collagen I expression (dedifferentiation marker) decreased dramatically after alginate entrapment, while reduced oxygen tension had no effect. It is concluded that alginate encapsulation and reduced oxygen tension help restore key differentiated phenotypic markers of passaged chondrocytes. These findings have important implications for cartilage tissue engineering, since they enable the increase in differentiated cell numbers needed for the in vitro development of functional cartilaginous tissue suitable for implantation.
Identification of Integrin-like Matrix Receptors with Affinity for Interstitial Collagens (1989)

Antibodies to a rat liver membrane glycoprotein with an Mr of 115,000 (nonreduced) inhibited the attachment of rat hepatocytes and primary rat heart fibroblasts to both collagen and fibronectin. The Mr 115,000 glycoprotein cross-reacted immunologically with the beta 1-chain of the rat hepatocyte fibronectin receptor (HFNR), and the two proteins showed identical peptide maps after proteolytic cleavage. It was concluded that the Mr 115,000 protein was similar or identical to the beta 1-chain of Arg-Gly-Asp (RGD)-directed matrix receptors. Although collagen type I contains several RGD sequences, the attachment of hepatocytes and fibroblasts to collagen type I was not inhibited by the synthetic peptide GRGDTP in concentrations that blocked adhesion to fibronectin. Furthermore, hepatocytes adhered equally well to collagen fragments, generated by cyanogen bromide cleavage, lacking RGD sequences as to fragments containing this sequence. Antibodies to the Mr 115,000 protein inhibited the adhesion of hepatocytes to both types of collagen fragments. Taken together, these data indicate the presence of collagen receptors that share the beta-subunit with the HFNR but that are not directed to RGD sequences. Tentative alpha-chains of the collagen matrix receptor complex were isolated by immunoprecipitation of surface 125I-labeled fibroblast membrane proteins purified by affinity chromatography on immobilized collagen type I. Data are presented indicating that proteins with Mr around 145,000 and 170,000 (nonreduced) are associated in noncovalently linked complexes with the Mr 115,000 protein. These complexes have affinity for collagen and thus have properties expected for integrin-like collagen receptors.
On 5G Radio Access Network Slicing: Radio Interface Protocol Features and Configuration (2018)

Network slicing is a fundamental capability for future Fifth Generation (5G) networks to facilitate the cost-effective deployment and operation of multiple logical networks over a common physical network infrastructure in a way that each logical network (i.e. network slice) can be customized and dimensioned to best serve the needs of specific applications (e.g. mobile broadband, smart city, connected car, public safety, fixed wireless access) and users (e.g. general public, enterprise customers, virtual operators, content providers). The practical realization of such capability still raises numerous technical challenges, both in the Core and RAN parts of the 5G system. Through a comprehensive analysis of the impact that the realization of RAN slicing has on the different layers of the radio interface protocol architecture, this article proposes a framework for the support and specification of RAN slices based on the definition of a set of configuration descriptors that characterize the features, policies and resources to be put in place across the radio protocol layers of a next-generation RAN node.
Fast H.264/AVC to HEVC transcoding using mode merging and mode mapping (2015)

A fast H.264/AVC to HEVC transcoding algorithm using motion vector based mode merging and conditional probability based mode mapping is proposed in this paper. Reusing the incoming H.264 block modes directly does not give the best Rate Distortion performance in HEVC. Motion vector variance is used to predict the possible merge of the H.264 blocks at coding depths 0 and 1 in HEVC. A conditional probability based mathematical model is developed to predict the two most probable modes at coding depths 2 and 3 of the HEVC encoding stage. Results show around 50 percent complexity reduction with negligible rate distortion loss.
Specifying label layout style by example (2007)

Creating high-quality label layouts in a particular visual style is a time-consuming process. Although automated labeling algorithms can aid the layout process, expert design knowledge is required to tune these algorithms so that they produce layouts which meet the designer's expectations. We propose a system which can learn a labellayout style from a single example layout and then apply this style to new labeling problems. Because designers find it much easier to create example layouts than tune algorithmic parameters, our system provides a more natural workflow for graphic designers. We demonstrate that our system is capable of learning a variety of label layout styles from examples.
Development and advantages of serum-free, chemically defined nutrient media for culturing of nerve tissue (1988)

This paper describes in a historical perspective the development of serum-free nutrient media suitable for long-term culturing of nerve tissue. Several disadvantages of the use of serum are discussed, coupled with an acknowledgement that it is not always advisable to replace a routinely used serum-supplemented medium by a chemically defined medium with the expectation of immediate success. Therefore a strategy is given on how to develop a chemically defined medium that is thoroughly tuned to the specific needs of the cell type to be cultured. It is argued that such a medium has several substantial advantages over the use of serum.
A bidirectional multi-channel MAC protocol for improving TCP performance on multihop wireless ad hoc networks (2004)

The TCP protocol often suffers from performance problems in conventional single-channel multihop wireless ad hoc networks. The problems arise from hidden node and exposed node issues, which can lead to channel contention in the forward direction between TCP DATA packets that are part of the same TCP flow control window, as well as contention between TCP DATA and TCP ACK packets flowing in opposite directions. In this paper, we propose and evaluate a novel bidirectional multi-channel MAC protocol designed to improve TCP performance over a multihop wireless network. The protocol uses multiple transmission channels at the physical layer to reduce TCP DATA-DATA contention, and bidirectional RTS/CTS channel reservations to reduce TCP DATA-ACK collisions. With four channels, simulation results for static multihop networks show TCP throughput gains of 50% to 180%, compared to a conventional IEEE 802.11 MAC protocol. Fairness is also improved with our protocol, since contention is confined to a short handshake period on the control channel.
Fully Automatic Quantification of Microarray Image Data (2002)

DNA microarrays are now widely used to measure expression levels and DNA copy number in biological samples. Ratios of relative abundance of nucleic acids are derived from images of regular arrays of spots containing target genetic material to which fluorescently labeled samples are hybridized. Whereas there are a number of methods in use for the quantification of images, many of the software systems in wide use either encourage or require extensive human interaction at the level of individual spots on arrays. We present a fully automatic system for microarray image quantification. The system automatically locates both subarray grids and individual spots, requiring no user identification of any image coordinates. Ratios are computed based on explicit segmentation of each spot. On a typical image of 6000 spots, the entire process takes less than 20 sec. We present a quantitative assessment of performance on multiple replicates of genome-wide array-based comparative genomic hybridization experiments. By explicitly identifying the pixels in each spot, the system yields more accurate estimates of ratios than systems assuming spot circularity. The software, called, runs on Windows platforms and is available free of charge for academic use.
Correlation of Particular Bacterial PCR-Denaturing Gradient Gel Electrophoresis Patterns with Bovine Ruminal Fermentation Parameters and Feed Efficiency Traits (2010)

The influence of rumen microbial structure and functions on host physiology remains poorly understood. This study aimed to investigate the interaction between the ruminal microflora and the host by correlating bacterial diversity with fermentation measurements and feed efficiency traits, including dry matter intake, feed conversion ratio, average daily gain, and residual feed intake, using culture-independent methods. Universal bacterial partial 16S rRNA gene products were amplified from ruminal fluid collected from 58 steers raised under a low-energy diet and were subjected to PCR-denaturing gradient gel electrophoresis (DGGE) analysis. Multivariate statistical analysis was used to relate specific PCR-DGGE bands to various feed efficiency traits and metabolites. Analysis of volatile fatty acid profiles showed that butyrate was positively correlated with daily dry matter intake (P < 0.05) and tended to have higher concentration in inefficient animals (P = 0.10), while isovalerate was associated with residual feed intake (P < 0.05). Our results suggest that particular bacteria and their metabolism in the rumen may contribute to differences in host feed efficiency under a low-energy diet. This is the first study correlating PCR-DGGE bands representing specific bacteria to metabolites in the bovine rumen and to host feed efficiency traits.