gebhart/patrepeval huggingface dataset:
Plasmid DNA Electrotransfer: A New Non Viral Method for Gene Therapy in Oncology (2002)

With some defined conditions, electric pulses delivery to tissue in vivo can greatly enhance DNA transfection. We here describe the application in oncology of intratumoral or intramuscular DNA electrotransfer by using muscle as a secretory tissue of transgenic proteins displaying anticancer properties.
Formation of covalent complexes between human<i>O<sup>6</sup></i>alkyltransferase and BCNU-treated defined length synthetic oligodeoxynucleotides (1988)

Repair of chloroethylnitrosourea (CENU)-induced precursors of DNA interstrand cross-links by O6-alkylguanine-DNA alkyltransferase (GAT or GATase) appears to be a factor in tumor resistance to therapy with this class of antineoplastic drugs. Since human GAT is highly specific for O6-guanine, yet the probable cross-link structure is N'-Guanine N3-cytosine ethane, rearrangement of the initial O6-guanine adduct via O6,N1ethanoguanine has been proposed. We suggested that GAT reaction with this intermediate would produce DNA covalently linked to protein through an ethane link from N1-guanine to the alkylacceptor site on GAT. In preliminary studies we demonstrated a covalent complex between GAT and carmustine (BCNU)-treated DNA by a precipitation assay method. We have now developed a method for isolating the reaction product of BCNU-treated synthetic 14-mer [32P]-labeled oligodeoxynucleotide and GAT using polyacrylamide gel electrophoresis. This approach can be used to characterize the adducts induced by CENUs that lead to complex formation with GAT. Results obtained to date are consistent with these adducts being precursors of DNA interstrand cross-links.
Synthetic heterodyne detection in a fiber-optic ring-laser gyro (1993)

A synthetic heterodyne detection scheme for ring-laser gyros to sense both rotation rate and direction is presented. This scheme is compatible with the sinusoidal push-pull phase-modulation technique for suppressing frequency locking. The good experimental results achieved in a fiber-optic Brillouin ring-laser gyro show the potential of this detection scheme in applications.
Engineering of Complex Order and the Macroscopic Deformation of Liquid Crystal Polymer Networks (2012)

Rise or fall: Complex-structured freestanding polymer films with molecular order in three dimensions were prepared through photoalignment of polymerizable liquid crystals. The resulting films deform into cone and saddle shapes upon heating.
Lyophilization of Semiconducting Polymer Dot Bioconjugates (2013)

Semiconducting polymer dot (Pdot) bioconjugates are a new class of ultrabright fluorescent probes. Here, we report a procedure for lyophilizing Pdot bioconjugates so that they successfully retain their optical properties, colloidal stability, and cell-targeting capability during storage. We found that, when Pdot bioconjugates were lyophilized in the presence of 10% sucrose, the rehydrated Pdot bioconjugates did not show any signs of aggregation and exhibited the same hydrodynamic diameters as before lyophilization. The brightness of the lyophilized Pdots was at least as good as before lyophilization, but in some cases, the quantum yield of lyophilized Pdots curiously showed an improvement. Finally, using flow cytometry, we demonstrated that lyophilized Pdot bioconjugates retained their biological targeting properties and were able to effectively label cells; in fact, cells labeled with lyophilized Pdot bioconjugates composed of PFBT, which were stored for 6 months at -80 °C, were ~22% brighter than those labeled with identical but unlyophilized Pdot bioconjugates. These results indicate lyophilization may be a preferred approach for storing and shipping Pdot bioconjugates, which is an important practical consideration for ensuring Pdots are widely adopted in biomedical research.
A Protective Vaccine Delivery System for <i>In Vivo</i> T Cell Stimulation Using Nanoengineered Polymer Hydrogel Capsules (2009)

Successful delivery of labile vaccine antigens, such as peptides and proteins, to stimulate CD4 and CD8 T cell immunity could improve vaccine strategies against chronic infections such as HIV and Hepatitis C. Layer-by-layer (LbL)-assembled nanoengineered hydrogel capsules represent a novel and promising technology for the protection and delivery of labile vaccine candidates to antigen-presenting cells (APCs). Here we report on the in vitro and in vivo immunostimulatory capabilities of LbL-assembled disulfide cross-linked poly(methacrylic acid) (PMA(SH)) hydrogel capsules as a delivery strategy for protein and peptide vaccines using robust transgenic mice models and ovalbumin (OVA) as a model vaccine. We demonstrate that OVA protein as well as multiple OVA peptides can be successfully encapsulated within nanoengineered PMA(SH) hydrogel capsules. OVA-containing PMA(SH) capsules are internalized by mouse APCs, resulting in presentation of OVA epitopes and subsequent activation of OVA-specific CD4 and CD8 T cells in vitro. OVA-specific CD4 and CD8 T cells are also activated to proliferate in vivo following intravenous vaccination of mice with OVA protein- and OVA peptide-loaded PMA(SH) hydrogel capsules. Furthermore, we show that OVA encapsulated within the PMA(SH) capsules resulted in at least 6-fold greater proliferation of OVA-specific CD8 T cells and 70-fold greater proliferation of OVA-specific CD4 T cells in vivo compared to the equivalent amount of OVA protein administered alone. These results highlight the potential of nanoengineered hydrogel capsules for vaccine delivery.
Things go better with coke: the beneficial role of carbonaceous deposits in heterogeneous catalysis (2015)

Carbonaceous deposits on heterogeneous catalysts are traditionally associated with catalyst deactivation. However, they can play a beneficial role in many catalytic processes,<italic>e.g.</italic>dehydrogenation, hydrogenation, alkylation, isomerisation, Fischer–Tropsch, MTO<italic>etc.</italic>This review highlights the role and mechanism by which coke deposits can enhance catalytic performance.
Diabetes-Induced Accelerated Atherosclerosis in Swine (2001)

Patients with diabetes are at higher risk for atherosclerotic disease than nondiabetic individuals with other comparable risk factors. Studies examining mechanisms underlying diabetes-accelerated atherosclerosis have been limited by the lack of suitable humanoid animal models. In this study, diabetes was superimposed on a well-characterized swine model of atherosclerosis by injection of the beta-cell cytotoxin streptozotocin (STZ), resulting in a >80% reduction in beta-cells and an increase in plasma glucose to diabetic levels. Animals were maintained without exogenous insulin for up to 48 weeks. Plasma glucose and cholesterol levels and lesion extent and severity were quantified in swine with diabetes and hyperlipemia alone and in combination compared with controls. Diabetes had no effect on plasma cholesterol levels, but diabetic/hyperlipemic (D-HL) swine developed hypertriglyceridemia and showed a doubling in aortic sudanophilia over nondiabetic/hyperlipemic (N-HL) swine as early as 12 weeks (47.25 +/- 4.5 vs. 24.0 +/- 4.6%). At 20 weeks, coronary artery stenosis was significantly greater in D-HL than in N-HL animals (86 +/- 10 vs. 46 +/- 8%). Coronary lesions predominantly arose in the first 2-3 cm of the vessels and displayed humanoid morphology. Aortic lesions in D-HL swine had double the cholesterol content of those in N-HL swine, and incorporation of oleate into cholesteryl ester was significantly greater in grossly normal aortic areas of D-HL swine compared with N-HL and was attributed to similar elevated incorporation in monocytes. This large study demonstrates that a model of diabetes with humanoid characteristics, including hypertriglyceridemia and severe, accelerated atherosclerosis can be reproducibly induced and maintained in swine. This model should potentially be of great value in elucidating mechanisms underlying the accelerated atherosclerosis seen in human diabetic individuals.
Strategies for Robotic Sensing Using Acoustics (1985)

Robotics is an acknowledged critical component of present and future manufacturing methods. The competitive drive for still higher productivity is pressing robotics technology to provide machine intelligence capable of accurate, real-time adaptive behavior. Sensing is considered a major component of machine intelligence. For manufacturing, repair and maintenance, a sense of touch, proximity and depth perception, traditionally considered uniquely human activities, are among the key requirements for any machine to emulate - or surpass. Acoustic and ultrasonic based sensors are demonstrating a capacity to provide perceptual information for range, object recognition, tactile force and shape determination. They also complement machine vision. Numerous systems that make use of acousticshltrasonics and/or rely on the piezoelectric properties of transduction sensors will be examined: The device physics, data acquisition, signal processing, and feature extraction capabilities are always interrelated. Future requirements for systems design, which affects the speed of machine adaptive behavior, will also be discussed. The reader will note from the current state of the art that a great deal more research remains to be done.
Call Setup Latency Analysis in SIP-Based Voice over WLANs (2008)

In this letter, we consider session initiation protocol (SlP)-based voice over wireless local area networks (VoWLANs). We derive the analytical expression for the average call setup latency, and we observe the impact of the number of contending mobile nodes in a WLAN. Extensive simulation results are given to validate the analytical results.
Understanding Data Persistence (2019)

All but the simplest apps need to store data. The Stocks app lets users track their favorite stocks, so it needs to store the list of stocks to follow that the user chose. Each time the user launches the Stocks app again, it displays the list of stocks the user inputted previously. If the user adds or deletes stocks from this list, the Stocks app needs to store this updated list and retrieve it again the next time the user loads the Stocks app.
El Islam político: ¿está listo para comprometerse? (2005)

beta-interferon (beta-IFN) has both pro and anti-inflammatory properties, the balance of which leads to some suppression of disease activity in multiple sclerosis patients. Here, we examine the immunomodulation of neonatal rodent microglia, the principal CNS accessory cell, by beta-IFN and consider the interaction of beta-IFN and gamma-interferon (gamma-IFN). beta-IFN and gamma-IFN inhibit microglial proliferation. beta-IFN antagonises both gamma-IFN-induced upregulation of class II expression and the ability of gamma-IFN primed cells to mount a respiratory burst. In contrast, beta-IFN upregulates microglial Fc receptor expression and augments tumour necrosis factor alpha secretion from suboptimally stimulated microglia.
Modulation of human platelet function by food flavonoids (1996)

Conference Article| May 01 1996 Modulation of human platelet function by food flavonoids COLLETE KELLY; COLLETE KELLY 1Division of Biochemical Sciences. Rowett Research Institute, Aberdeen, AB2 9SB, Scotland, U.K. Search for other works by this author on: This Site PubMed Google Scholar KIRSTY HUNTER; KIRSTY HUNTER 1Division of Biochemical Sciences. Rowett Research Institute, Aberdeen, AB2 9SB, Scotland, U.K. Search for other works by this author on: This Site PubMed Google Scholar LYNN CROSBIE; LYNN CROSBIE 1Division of Biochemical Sciences. Rowett Research Institute, Aberdeen, AB2 9SB, Scotland, U.K. Search for other works by this author on: This Site PubMed Google Scholar MARGARET J. GORDON; MARGARET J. GORDON 1Division of Biochemical Sciences. Rowett Research Institute, Aberdeen, AB2 9SB, Scotland, U.K. Search for other works by this author on: This Site PubMed Google Scholar ASIM K. DUTTA-ROY ASIM K. DUTTA-ROY 1Division of Biochemical Sciences. Rowett Research Institute, Aberdeen, AB2 9SB, Scotland, U.K. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1996 Biochemical Society1996 Biochem Soc Trans (1996) 24 (2): 197S. https://doi.org/10.1042/bst024197s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation COLLETE KELLY, KIRSTY HUNTER, LYNN CROSBIE, MARGARET J. GORDON, ASIM K. DUTTA-ROY; Modulation of human platelet function by food flavonoids. Biochem Soc Trans 1 May 1996; 24 (2): 197S. doi: https://doi.org/10.1042/bst024197s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1996 Biochemical Society1996 Article PDF first page preview Close Modal You do not currently have access to this content.
The least squares estimation of time delay and its use in signal detection (1978)

This paper examines the use of two spatially separated receivers to determine the presence of a distant signal source and its relative bearing. Ideally, the phase shift between the receivers' output is proportional to the frequency with the time delay between outputs equal to the proportionality constant. Because of noise, the plot of phase against frequency is scattered along a straight line whose slope is the time delay. A least squares estimator of the slope turns out to be equivalent to the maximum likelihood estimator developed by Hamon and Hannan [1]. Since the goodness of fit of the least squares line is a function of the coherence between the receivers' output, the sum of the squared errors is used as a test statistic in detection. The proposed detector has a detection threshold that depends only on the probability of false alarm and not on the ambient noise level. It can also be simply extended to an array of receivers.
Protective Efficacy of a DNA Vaccine Encoding Antigen 85A from <i>Mycobacterium bovis</i> BCG against Buruli Ulcer (2001)

Buruli ulcer, caused by Mycobacterium ulcerans, is characterized by deep and necrotizing skin lesions, mostly on the arms and legs. Together with tuberculosis and leprosy, this mycobacterial disease has become a major health problem in tropical and subtropical regions, particularly in central and western Africa. No specific vaccine is available for Buruli ulcer. There is, however, evidence in the literature that suggests a cross-reactive protective role of the tuberculosis vaccine M. bovis BCG. To identify potential mechanisms for this cross-protection, we identified and characterized the M. ulcerans homologue of the important protective mycobacterial antigen 85 (Ag85A) from BCG. The homologue is well conserved in M. ulcerans, showing 84.1% amino acid sequence identity and 91% conserved residues compared to the sequence from BCG. This antigen was sufficiently conserved to allow cross-reactive protection, as demonstrated by the ability of M. ulcerans- infected mice to exhibit strong cellular immune responses to both BCG and its purified Ag85 complex. To further address the mechanism of cross-reactive protection, we demonstrate here that prior vaccination with either BCG or plasmid DNA encoding BCG Ag85A is capable of significantly reducing the bacterial load in the footpads of M. ulcerans- infected mice, as determined by Ziehl-Neelsen staining and by actual counting of CFU on 7H11 Middlebrook agar. Together, the results reported here support the potential of a cross-protective Ag85-based future vaccine against tuberculosis, Buruli ulcer, and leprosy.
Health Benefits of Rice Bran - A Review (2011)

Rice bran along with the germ is an inherent part of whole grain which consists of phytonutrients like oryzanols, tocopherols, tocotrienols, phytosterols and importantly dietary fibers. The complete exploitation of its potential has not been realized due to problems associated with rancidity. However, owing to numerous stabilization procedures, it has been possible to derive an array of health-promoting value-added products. The applications span over a wide range starting from cholesterol reduction, combating cancer, alleviating menopausal and postmenopausal symptoms, masking the signs of ageing to production of PHA substitutes and treating water from agricultural run-off. The most commonly used form is its oil that has exceptional properties which makes it unparalleled when compared with other vegetable oils. This article gives a bird’s eye view of rice bran and its distinct properties.
MMC05-6: PACA: Peer-Assisted Channel Assignment for Home Wireless LANs (2006)

A home network is a residential local area network, in which users are networking their PCs, laptops or wireless multimedia appliances to use a single residential access point (AP) or gateway connecting to the Internet. As network-capable appliances continue to gain momentum in the home, home networks are becoming increasingly popular. The problem arising from the increasing number of "wireless home" is the interference introduced from neighbor's home networks which leads to throughput dropped precipitously. This problem is different from the traditional channel assignment problem in WLAN. We are considering how home wireless network can self-configure its operating channel to minimize interference with other networks instead of how network administrator assigns channel to different APs. In this paper, we propose a peer-assisted channel assignment algorithm, termed PACA, for home wireless LAN based on local information. A newly power on AP automatically configures itself to operate on a channel with least interference. Mobile node helps other APs to do channel assignment and switching dynamically by providing traffic load information. Our simulation and experimental measurements show that the algorithm reduces interference among different networks and improves user throughput.
Property/composition relationships for Hanford high-level waste glasses melting at 115{degrees}C volume 1: Chapters 1-11 (1994)

A Composition Variation study (CVS) is being performed within the Pacific Northwest Laboratory Vitrification Technology Development (PVTD) project in support of a future high-level nuclear waste vitrification plant at the Hanford site in Washington. From 1989 to 1994, over 120 nonradioactive glasses were melted and properties measured in five statistically-designed experimental phases. Glass composition is represented by the 10 components SiO{sub 2}, B{sub 2}O{sub 3}, Al{sub 2}O{sub 3}, Fe{sub 2}O{sub 3}, ZrO{sub 2}, Na{sub 2}O, Li{sub 2}O, CaO, MgO, and Others (all remaining components). The properties measured include viscosity ({eta}), electrical conductivity ({epsilon}), glass transition temperature (T{sub g} ), thermal expansion of solid glass ({alpha}{sub s}) and molten glass ({alpha}{sub m}), crystallinity (quenched and canister centerline cooled glasses), liquidus temperature (T{sub L}), durability based on normalized elemental releases from the Materials Characterization Center-1 28-day dissolution test (MCC-1, r{sub mi}) and the 7-day Product Consistency Test (PCT, r{sub pi}), and solution pHs from MCC-1 and PCT. Amorphous phase separation was also evaluated. Empirical first- and second-order mixture models were fit using the CVS data to relate the various properties to glass composition. Equations for calculating the uncertainty associated with property values predicted by the models were also developed. The models were validated using both internal and external data. Other modeling approaches (e.g., non-bridging oxygen, free energy of hydration, phase-equilibria T{sub L}) were investigated for specific properties. A preliminary Qualified Composition Region was developed to identify glass compositions with high confidence of being processable in a melter and meeting waste form acceptance criteria.
Full-vector analysis of a realistic photonic crystal fiber (1999)

We analyze the guiding problem in a realistic photonic crystal fiber, using a novel full-vector modal technique. This is a biorthogonal modal method based on the non-self-adjoint character of the electromagnetic propagation in a fiber. Dispersion curves of guided modes for different fiber structural paremeters are calculated, along with the two-dimensional transverse intensity distribution of the fundamental mode. Our results match those achieved in recent experiments in which the feasibility of this type of fiber was shown.
Quantitation of membrane-protein-solute interactions during ultrafiltration. (1978)

One of the recommendations in the International Bamboo Congress and Workshop, held\nat Bali in 1995, requested the International Network for Bamboo and Rattan (INBAR), "to organize a task force to discuss and finalize a building code for bamboo". Consequently a draft was prepared under the title, "An International Model for National Building Codes, Section Bamboo". The target group of this Code comprises architects, builders, designers and engineers. These people are already familiar with national building codes for timber, masonry, etc. For bamboo, however, a similar Code is missing and this is an obstacIe for the recognition of bamboo as a building and engineering material. After approval by the next International Bamboo Congress and Workshop, the document will be submitted to the International Organization for Standardization (ISO). Once it has been accepted\nas an ISO-Standard, it can serve as the model or basis for developing National Building Codes by countries that grow bamboo and use it in construction. The topics incIuded in the Code are: philosophy, design concepts, structural design, beams, columns, joints, trusses, panels, durability, fire protection, grading and quality control.
Software radio: a modern approach to radio engineering (2002)

Software-based approaches enable engineers to build wireless system radios that are easier to manufacture, more flexible, and more cost-effective. Software Radio: A Modern Approach to Radio Engineering systematically reviews the techniques, challenges, and tradeoffs of DSP software radio design. Coverage includes constructing RF front-ends; using digital processing to overcome RF design problems; direct digital synthesis of modulated waveforms; A/D and D/A conversions; smart antennas; object-oriented software design; and choosing among DSP microprocessors, FPGAs, and ASICs. This is an excellent book for all RF and signal processing engineers building advanced wireless systems.