gebhart/patrepeval huggingface dataset:
Direct Demonstration of Dielectric Breakdown in the Membranes of Valonia utricularis (1975)

Abstract It is shown that if the membrane potential of cells of Valonia utricularis was increased rapidly by applying ~500 μs current pulses, dielectric breakdown of the membrane occured when the potential reached a value of ~0.85 V. The breakdown phenomenon observed was not associated with global damage to the cell or its membrane. The process could be repeated after a short resealing time (~ 10s), many times on a single cell. The rapidity of the breakdown process (~1 μs) rules out the possibility that dielectric breakdown occurs by a mechanism similar to that of punch-through, which involves changes in ionic profiles.
Predictive value of in vitro assessment of cytotoxic drug activity in advanced breast cancer (2005)

The predictive value of a short-term in vitro total cell kill assay was investigated in 37 patients with breast cancer (BC). Tumor cells were prepared from tumor samples from 17 patients with locally advanced and 20 with metastatic BC, which were treated with the FEC (5-fluorouracil, epirubicin, cyclophosphamide) regimen or a combination of epirubicin and taxane. The cells were then tested in the fluorometric microculture cytotoxicity assay (FMCA), which is based on the conversion by viable cells of fluorescein diacetate to fluorescent fluorescein, for sensitivity to the drugs given in vivo. The FMCA data were scored as low, intermediate or extreme drug resistance based on the median cell survival +/- SD for each drug and patient subset. The drug classification for each sample was then correlated to clinical outcome in terms of objective response and time to tumor progression. The FMCA significantly predicted objective tumor response with a sensitivity of 89% and a specificity of 53%. Furthermore, in patients with locally advanced BC, low drug resistance was significantly associated with longer time to progression. It is concluded that the FMCA seems to report clinically relevant cytotoxic drug sensitivity data in BC. The potential clinical role of the FMCA and similar tests is discussed.
Specificity of cleavage in replicative-form DNA of bovine herpesvirus 1 (1988)

The linear double-stranded DNA genome of herpesvirus as it is present in infectious virions needs to be circularized after infection of host cells and before DNA replication. Replicative-form genomes have to be cleaved into linear unit-length molecules during virion maturation and are most probably the substrate for inversion of the short segment relative to the long segment of the bovine herpesvirus 1 (BHV-1) genome. Those regions of the BHV-1 genome which are functionally involved in these processes have been analyzed at the molecular level by cloning and sequencing the genomic termini, the fusion of both termini from replicative-form molecules, and the junction between the short and the long genome segment. On the basis of the simple genome arrangement of BHV-1, it was inferable that the cleavage of replicative-form genomes by a hypothetical BHV-1 terminase activity may be specified by a sequence at the left end of UL (An element), which is located proximal to a reiterated beta element that makes up the cleavage site itself. The relationship of those elements in BHV-1 and the comparison to similar regions of other herpesviruses indicate consensus sequence elements which are functionally important for cleavage and isomerization of viral DNA during maturation of virions.
Identification of MpaA, an Amidase in <i>Escherichia coli</i> That Hydrolyzes the γ- <scp>d</scp> -Glutamyl- <i>meso-</i> Diaminopimelate Bond in Murein Peptides (2003)

MpaA amidase was identified in Escherichia coli by its amino acid sequence homology with the ENP1 endopeptidase from Bacillus sphaericus. The enzymatic activity of MpaA, i.e., hydrolysis of the gamma-D-glutamyl-diaminopimelic acid bond in the murein tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelic acid, was demonstrated in the cell extract of a strain expressing mpaA from a multicopy plasmid. An mpaA mpl (murein peptide ligase) double mutant accumulated large amounts of murein tripeptide in its cytoplasm, consistent with the premise that MpaA degrades the tripeptide if its recycling via the peptidoglycan biosynthetic pathway is blocked.
A Powerful Approach for the Selection of 2-Aminopurine Substitution Sites to Investigate RNA Folding (2011)

A precise tertiary structure must be adopted to allow the function of many RNAs in cells. Accordingly, increasing resources have been devoted to the elucidation of RNA structures and the folding of RNAs. 2-Aminopurine (2AP), a fluorescent nucleobase analogue, can be substituted in strategic positions of DNA or RNA molecules to act as site-specific probe to monitor folding and folding dynamics of nucleic acids. Recent studies further demonstrated the potential of 2AP modifications in the assessment of folding kinetics during ligand-induced secondary and tertiary RNA structure rearrangements. However, an efficient way to unambiguously identify reliable positions for 2AP sensors is as yet unavailable and would represent a major asset, especially in the absence of crystallographic or NMR structural data for a target molecule. We report evidence of a novel and direct correlation between the 2'-OH flexibility of nucleotides, observed by selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) probing and the fluorescence response following nucleotide substitutions by 2AP. This correlation leads to a straightforward method, using SHAPE probing with benzoyl cyanide, to select appropriate nucleotide sites for 2AP substitution. This clear correlation is presented for three model RNAs of biological significance: the SAM-II, adenine (addA), and preQ(1) class II (preQ(1)cII) riboswitches.
Early Changes in Microbial Community Structure Are Associated with Sustained Remission After Nutritional Treatment of Pediatric Crohnʼs Disease (2016)

Microbial community structure differs between healthy controls, patients who have an enduring response to exclusive enteral nutrition, and those who relapse early on introduction of normal diet. Our novel Bayesian approach to these differences is able to predict sustained remission after exclusive enteral nutrition.
Integrating RFID (2004)

RFID (radio frequency identification) has received a great deal of attention in the commercial world over the past couple of years. The excitement stems from a confluence of events. First, through the efforts of the former Auto-ID Center and its sponsor companies, the prospects of low-cost RFID tags and a networked supply chain have come within reach of a number of companies. Second, several commercial companies and government bodies, such as Wal-Mart and Target in the United States, Tesco in Europe, and the U.S. Department of Defense, have announced RFID initiatives in response to technology improvements.
Germ-line mutations in the neurofibromatosis 2 gene: correlations with disease severity and retinal abnormalities. (1996)

Neurofibromatosis 2 (NF2) features bilateral vestibular schwannomas, other benign neural tumors, and cataracts. Patients in some families develop many tumors at an early age and have rapid clinical progression, whereas in other families, patients may not have symptoms until much later and vestibular schwannomas may be the only tumors. The NF2 gene has been cloned from chromosome 22q; most identified germ-line mutations result in a truncated protein and severe NF2. To look for additional mutations and clinical correlations, we used SSCP analysis to screen DNA from 32 unrelated patients. We identified 20 different mutations in 21 patients (66%): 10 nonsense mutations, 2 frameshifts, 7 splice-site mutations, and 1 large in-frame deletion. Clinical information on 47 patients from the 21 families included ages at onset and at diagnosis, numbers of meningiomas, spinal and skin tumors, and presence of cataracts and retinal abnormalities. We compared clinical findings in patients with nonsense or frameshift mutations to those with splice-site mutations. When each patient was considered as an independent random event, the two groups differed (P < or = .05) for nearly every variable. Patients with nonsense or frameshift mutations were younger at onset and at diagnosis and had a higher frequency and mean number of tumors, supporting the correlation between nonsense and frameshift mutations and severe NF2. When each family was considered as an independent random event, statistically significant differences between the two groups were observed only for mean ages at onset and at diagnosis. A larger data set is needed to resolve these discrepancies. We observed retinal hamartomas and/or epiretinal membranes in nine patients from five families with four different nonsense mutations. This finding, which may represent a new genotype-phenotype correlation, merits further study.
Thermal annealing effects on silver nanowire networks (2014)

Thermal annealing is shown to be a successful approach to reduce the electrical resistance of transparent electrodes made of randomly oriented silver nanowires (AgNWs). A decrease in the electrical resistance by several orders of magnitude, whilst maintaining optical transmission (above 85%), is demonstrated. Several mechanisms involved in the electrical behaviour induced by thermal treatment both in air and under vacuum are identified using a combination of ramped, stepped and isothermal annealing. Some mechanisms lead to the reduction of the electrical resistance such as local sintering, while others, such as spheroidisation, induce irreversible damage to the network. It is also shown that the polymer used in the synthesis of Ag nanowires plays a crucial role as a thermal stabiliser under vacuum conditions. Finally, optimised samples exhibit an optical transmittance of 83% (without substrate contribution removal) and a sheet resistance of 9.5 Ω/sq.
Renewing Human Assets in Indian Ports: Issues and Strategies for Improving Performance (2005)

The main objective of this research study is to examine (i) critical issues relating to renewing human assets for improving port performance, (ii) strategies to be taken for imparting training which would result in improving performance for Indian ports, and (iii) the existing relationship between port efficiency and renewing human assets through training in Indian Ports. A statistical analysis through a model comprising trainee density and average turnround of ships on port accounts in days has been carried out for data over three years 1999–00, 2000–01 and 2001–02. The results obtained have been very interesting. Three distinct relationships have emerged between renewal of human assets and port efficiency for three segments of observations. The author has then identified the issues and suggests the framework of policy and a plan for renewal of human assets for improving performance in Indian ports.
BGP Link-State Extensions for IPv6 Segment Routing (2017)

This document defines extensions to BGP link-state (BGP-LS) to carry
the information of IPv6 Segment Routing (SRv6).
Signature Pedagogy in Early Years Education: A Role for COTS Game-Based Learning (2012)

In this article we look at the links between early years pedagogy and the use of digital game-based learning. Early years education is a distinctive phase of the education system in many countries, generally covering the age range from 3–6 or 7 years. In the United Kingdom, it tends to bridge preschool and the first two years in primary school. Early years education is based on characteristic values, beliefs, and practices which distinguish it from later phases of mainstream educational provision. Based on an analysis of these characteristics, we argue that it is an example of a signature pedagogy. We look at the role of information and communication technologies in such pedagogy, specifically the use of digital games, illustrating the case with reference to a recent empirical study which looked at the use of a commercial off-the-shelf game with 5- and 6-year old children. We conclude by reaffirming the congruence between such games and a signature pedagogy of early years education. The role of the teacher, and in particular his/her pedagogical skill, is central to the educational use of commercial games.
Industrial Hygiene Evaluation Methods (2003)

Professionals and students in the field of industrial hygiene need a concise guide that thoroughly covers the practical methods of evaluating health threats in the workplace.Bisesi and Kohn's Industrial Hygiene Evaluation Methods, Second Edition introduces basic methods for evaluating work and some non-work environments in order to detect and measure physical, chemical and biological agents, as well as hazardous ergonomic factors. The book is divided into relatively short units that provide concise overviews and descriptions of basic concepts. Each unit is followed by practical technical exercises. These exercises foster the understanding of basic industrial hygiene principles and practices for collection, detection, identification, calculation, and interpretation of qualitative and quantitative data. Exercises can be conducted in a setting in which agents and other factors are detectable and measurable. Alternatively, the simulated evaluation exercises that are included can be conducted in a classroom or laboratory.This book is an introductory reference for environmental and occupational health and safety students and practitioners. It is an indispensable tool that illustrates methods fundamental to industrial hygiene practice, and is just as valuable in the professional's office as it is in the classroom.
A Target Assignment Problem (1957)

This paper is concerned with a target assignment model of a probabilistic and nonlinear nature, but nevertheless one which is closely related to the “personnel-assignment” problem. It is shown here that, despite the apparent nonlinearities, it is possible to devise a linear programming formulation that will ordinarily provide a close approximation to the original problem.(This abstract was borrowed from another version of this item.)
Collusion-resistant anonymous data collection method (2009)

The availability and the accuracy of the data dictate the success of a data mining application. Increasingly, there is a need to resort to on-line data collection to address the problem of data availability. However, participants in on-line data collection applications are naturally distrustful of the data collector as well as their peer respondents, resulting in inaccurate data collected as the respondents refuse to provide truthful data in fear of collusion attacks. The current anonymity-preserving solutions for on-line data collection are unable to adequately resist such attacks in a scalable fashion. In this paper, we present an efficient anonymous data collection protocol for a malicious environment such as the Internet. The protocol employs cryptographic and random shuffling techniques to preserve participants' anonymity. The proposed method is collusion-resistant and guarantees that an attacker will be unable to breach an honest participant's anonymity unless she controls all N-1 participants. In addition, our method is efficient and achieved 15-42% communication overhead reduction in comparison to the prior state-of-the-art methods.
Tramadol Release from a Delivery System Based on Alginate‐Chitosan Microcapsules (2003)

Abstract Alginate‐chitosan microcapsules to control the release of Tramadol‐HCl were prepared using two different methods. In the two‐stage procedure (Variant I) alginate was first pumped into a CaCl 2 /NaCl solution and then transferred into a chitosan solution. In the one‐stage procedure (Variant II) alginate was directly pumped into a chitosan/CaCl 2 solution, and different behavior could be noted in each case. The microcapsules were spherical in both variants and they swelled to a greater extent in a basic medium as compared to an acid one. The drug release profile of Tramadol from microcapsules in simulated gastric fluid and simulated intestinal fluid was also studied. The maximum release of Tramadol at 24 h was 64% and 86% for Variant I and II, respectively, in simulated intestinal fluid. Release was adjusted using the power law of the semi‐empirical Peppas equation in order to gain information about the release mechanism. In both cases the values of the exponent were found to be between 0.53 and 0.84 for swellable microcapsules in simulated gastric and intestinal fluids, respectively, indicating anomalous drug transport for both variants. The good results obtained with alginate‐chitosan microcapsules are comparable to those of the best products so far described in the scientific bibliography and in addition, chitosan is useful in pharmacy. Surface morphology of Tramadol‐loaded microcapsule. magnified image Surface morphology of Tramadol‐loaded microcapsule.
A Novel Voltammetric In-Situ Profiling System for ContinuousReal-Time Monitoring of Trace Elements in Natural Waters (1998)

This article describes a novel voltammetric in-situ profiling system (VIP System) for continuous, real-time monitoring of trace elements in fresh and seawater down to 500 meter depth. The VIP System has been designed using advanced microprocessor and telemetry technology. The heart of the submersible voltammetric probe is a Hg-plated Ir-based microsensor covered with an antifouling gel membrane. This gel integrated microsensor allows direct voltammetric measurements of trace metals in complex media without physical and chemical interferences of the test solution. A detailed description of the system is given and examples of environmental applications are reported for in-situ trace metal monitoring in oxygen saturated seawater as well as for in-situ profiling of MnII in anoxic lake water. The results indicate that the VIP System is robust and reliable and that in-situ measurements of mobile fraction of trace metals can be achieved down to very low concentration levels (ca. 5 ppt).
A laboratory-based survey of Campylobacter infections in Prahova County. (2008)

In developing countries, as well as in many western countries, members of the genus Campylobacter are recognized as one of the most common cause of acute bacterial enteritis. Campylobacter jejuni and Campylobacter coli isolation rates have been shown to be equal to, and sometimes higher than those of other enteric pathogens. The Microbiology Laboratory of the local Public Health Authority in Prahova County conducted a one and a half-year laboratory-based survey of Campylobacter infections in patients suffering from gastrointestinal symptoms. From a total of 3284 stool samples screened, the culture-positive ones confirmed the bacterial etiology for 551 diarrhea cases. Campylobacter was found in 345 specimens, being the most frequently isolated enteropathogen. C. jejuni outnumbered C. coli species (239 vs. 106 isolates). Salmonella isolates were the second local cause of diarrhea. The highest isolation rate of Campylobacter was found in children 5 years of age (262 strains). The prevalence of campylobacteriosis declined with age. The isolation rate of Campylobacter (10.5%), the unimodal age-specific distribution of cases, as well as the identification of polymicrobial infections among the screened population were epidemiological aspects resembling reports on campylobacteriosis in developing countries. The susceptibility of Campylobacter isolates to various antimicrobial agents, including macrolides and fluoroquinolones was also assessed. Among the screened isolates, Erythromycin retained a good activity, while an increased ciprofloxacin resistance was observed. The information gathered through this local study sustains the importance of Campylobacter in the etiology of autochthonous infectious diarrhea. A development of a national surveillance program regarding the most important foodborne pathogens would be beneficial for improving prevention and controlling measures.
Interocular suppression in cat striate cortex is not orientation selective (1995)

For the majority of neurones in cat striate cortex, the response to an optimal stimulus presented to one eye is suppressed when a stimulus of substantially different orientation is presented to the other eye. In order to determine the true orientational tuning of the underlying inhibitory interactions in the absence of binocular facilitation for matched stimuli, we tested how the response of such cells to an optimal grating in one eye is affected by gratings in the other eye of spatial frequencies too high or low to elicit an excitatory response through either eye: the vast majority of cells displayed suppression that was essentially independent of orientation. Our results indicate that interocular inhibition derives from cells representing all orientations, but is swamped by interocular facilitation for stimuli matched in orientation and spatial frequency.
Method for Heating Alkali Halides and Other Solids in Vapors of Controlled Pressure (1961)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation C. Z. Van Doorn; Method for Heating Alkali Halides and Other Solids in Vapors of Controlled Pressure. Rev. Sci. Instrum. 1 June 1961; 32 (6): 755–756. https://doi.org/10.1063/1.1717501 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioReview of Scientific Instruments Search Advanced Search |Citation Search
The yeast KRE5 gene encodes a probable endoplasmic reticulum protein required for (1----6)-beta-D-glucan synthesis and normal cell growth. (1990)

Yeast kre mutants define a pathway of cell wall (1----6)-beta-D-glucan synthesis, and mutants in genes KRE5 and KRE6 appear to interact early in such a pathway. We have cloned KRE5, and the sequence predicts the product to be a large, hydrophilic, secretory glycoprotein which contains the COOH-terminal endoplasmic reticulum retention signal, HDEL. Deletion of the KRE5 gene resulted in cells with aberrant morphology and extremely compromised growth. Suppressors to the KRE5 deletions arose at a frequency of 1 in 10(7) to 1 in 10(8) and permitted an analysis of deletions which were found to contain no alkali-insoluble (1----6)-beta-D-glucan. These results indicate a role for (1----6)-beta-D-glucan in normal cell growth and suggest a model for sequential assembly of (1----6)-beta-D-glucan in the yeast secretory pathway.
Regioselective Palladium-Catalyzed Arylation and Heteroarylation of Imidazo[1,2-<i>a</i>]pyridines (2006)

Palladium-catalyzed direct arylation and heteroarylation of imidazo[1,2-a]pyridines at the 3-position are described. The optimization of the reaction in conventional heating and the adaptation under microwaves irradiation is reported. The compatibility of the synthesis with the presence of bromo or chloro substituents in the 6-position was investigated.
Cellulose Acetate for Thermoplastic Foam Extrusion (2013)

Chapter 2
Predicting gene function using hierarchical multi-label decision tree ensembles (2010)

Our results suggest that decision tree based methods are a state-of-the-art, efficient and easy-to-use approach to ORF function prediction.