gebhart/patrepeval huggingface dataset:
Differential activation of p44<i>mapk</i> (ERK1) by α-thrombin and thrombin-receptor peptide agonist (1993)

alpha-Thrombin (thrombin), a potent mitogen for CCL39 hamster lung fibroblasts, stimulates phosphoinositide-specific phospholipase C (PI-PLC) and inhibits adenylate cyclase via cleavage of a specific G-protein-coupled receptor (TH-R), recently cloned from human and hamster cells. This action can be entirely mimicked by the synthetic peptide SFFLRNP, referred to here as TMP (thrombin-mimicking peptide). TMP corresponds to the first seven amino acids of the new N-terminus generated by thrombin cleavage of the hamster TH-R. Although thrombin and TMP apparently generate identical early transmembrane signals, only thrombin is mitogenic on its own. TMP needs to be associated with fibroblast growth factor (FGF), a tyrosine kinase-activating growth factor, to induce cell-cycle re-entry. Here, we have examined the early and late phase of p44 MAP kinase (p44mapk) activation in G0-arrested CCL39 cells after stimulation by thrombin, TMP, FGF or TMP+FGF. We found that: (i) both thrombin and TMP rapidly activate p44mapk in a dose-dependent manner with maximum activation at around 5 min, (ii) after the initial burst of activation, a second and long-lasting wave of activation is observed in response to thrombin (10-100 nM) but not to TMP (up to 300 microM), (iii) FGF alone (25 ng/ml), like thrombin, rapidly and persistently activates p44mapk (20-fold at 5 min and about 3-fold after 2 h), (iv) TMP added together with FGF strongly potentiates the second and sustained phase of p44mapk activation. From these results we propose that: (1) thrombin-induced mitogenesis is mediated only in part by the TH-R recently cloned and (2) activation of p44mapk, in particular the long-lasting phase that correlates with DNA synthesis, is an obligatory event for cell-cycle re-entry.
Experience With Nontreatment of Central Serous Choroidopathy (1974)

Data are presented from 27 patients (34 eyes) with central serous choroidopathy who were followed up for an average of 23 months without therapeutic intervention. The detachment completely resolved in all cases. The average duration of detachment was three months. In all eyes, the visual acuity returned to 20/40 or better, and in all but two eyes, the final visual acuity was 20/30 or better. Central serous choroidopathy is essentially a benign and self-limited disorder, and treatment by photocoagulation is seldom needed.
Parallelism and Garbage Collection Aware I/O Scheduler with Improved SSD Performance (2017)

In this paper, we propose PGIS, a parallelism and garbage collection aware I/O Scheduler, which identifies the hot data based on trace characteristics to exploit the channel level internal parallelism of flash-based storage systems. PGIS not only fully exploits abundant channel resource in the SSD, but also it introduces a hot data identification mechanism to reduce the garbage collection overhead. By dispatching hot read data to different channel, the channel level internal parallelism is fully exploited. By dispatching hot write data to the same physical block, the garbage collection overhead has been alleviated. The experiment results show that compared with existing I/O schedulers, PGIS improves the response time and garbage collection performance significantly. Consequently, PGIS reduces the garbage collection overhead up to 30.9%, while exploiting channel level internal parallelism.
Unexpected intramolecular cyclization of 4-(2-halophenyl)pyrazolo[3,4-b]quinolines: formation of 5- and 7-membered rings from one starter (2009)

Cyclization of 4-(2-halophenyl)-1H-pyrazolo[3,4-b]quinolines 3a-b and 9a-9b provided two regioisomeric compounds: 6-phenyl-6H-5,6,7-triazadibenzo[f,h]naphtho[3,2,1-cd]azulenes 4,10 and 1,3-diphenyl-3H-indeno[1,2,3-de]pyrazolo[3,4-b]quinolines 2,11. All of them are considered as new building blocks for optoelectronic materials. The two-step synthesis utilized readily available starting materials.
Cyclophosphamide-Induced Apoptosis in COV434 Human Granulosa Cells Involves Oxidative Stress and Glutathione Depletion (2007)

The anticancer drug cyclophosphamide induces granulosa cell apoptosis and is detoxified by glutathione (GSH) conjugation. We previously showed that both cyclophosphamide treatment and GSH depletion induced granulosa cell apoptosis in rats, but the role of GSH in apoptosis in human ovarian cells has not been studied. Using the COV434 human granulosa cell line, we tested the hypotheses that (1) GSH depletion or treatment with 4-hydroperoxycyclophosphamide (4HC), a preactivated form of cyclophosphamide, induces apoptosis, (2) GSH depletion potentiates 4HC-induced apoptosis, and (3) 4HC-induced apoptosis is mediated by GSH depletion and oxidative stress. Cells were treated with buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis, with or without follicle stimulating hormone (FSH) or serum. A significant increase in the number of apoptotic cells, assessed by terminal deoxynucleotidyl transferase-mediated deoxy-uridine triphosphate nick-end labeling (TUNEL) and Hoechst 33342 staining, occurred with BSO treatment. Treatment with 4HC dose-dependently induced apoptosis by TUNEL, Hoechst staining, and caspase 3 activation. Treatment with 4HC caused an increase in reactive oxygen species generation, measured by dichlorofluorescein fluorescence, oxidative DNA damage, measured by 8-hydroxyguanosine immunostaining, and an oxidation of the redox potential for the oxidized glutathione/reduced glutathione couple. Total intracellular GSH declined after 4HC treatment, preceding the onset of cell death. Treatment with antioxidants inhibited 4HC-induced apoptosis. Combined treatment with BSO and 4HC caused greater induction of apoptosis than either treatment alone. These findings are consistent with roles for oxidative stress and GSH depletion in mediating the induction of apoptosis in COV434 cells by cyclophosphamide.
Zur Kenntnis der β‐Dicarbonyl‐Verbindungen, VI. Mitteil.: Die Kondensation von α‐Aminomethylen‐β‐dicarbonyl‐Verbindungen mit Ketonen und mit β‐Dicarbonyl‐Verbindungen (1949)

Abstract Es Wird gezeigt, daß die Kondensation von α‐Aminomethylen‐β‐dicarbonyl‐Verbindungen mit Ketonen und mit β‐Dicarbonyl‐Verbindungen nach einem der Einwirkung von α‐Áthoxymethylen‐β‐dicarbonyl‐ bzw. Oxymethylen‐β‐carbonyl‐Verbindungen analogen Mechanismus verläuft; beide Reaktionen füren daher u.U. zu den gleichen Reaktionsprodukten.
C-4/BGA comparison with other MLC single chip package alternatives (2002)

Future applications will require higher I/O counts, more densification, lower cost and greater performance. This paper demonstrates why area-array based chip-to-substrate and substrate-to-card interconnections are strategic, particularly solder bump flip chips (SBFC or C-4) and solder grid arrays (SGA) respectively. That is, SBFC are capable of high pin counts coupled with high yields, performance and reliability. Moreover, recently introduced SGA interconnections, both ball and column, provide substantial benefits over standard pin grid array (PGA) packages. Also, SGA packages possess the highest density achievable at the card level when utilized in conjunction with SBFC-mounted die.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Service-oriented process control using High-Level Petri Nets (2008)

Service-oriented systems constitute a suitable approach for the development of modular, flexible and reconfigurable production systems, addressing the current requirements imposed by global markets. This paper focuses on the process control of Service-oriented production systems, whose behavior is regulated by the coordination of services that are available from distributed modular and collaborative control components. The proposed control is based on a kind of High-level Petri Nets tailored for the description, connection and synchronization of different concurrent processes. Several features can be distinguished by this approach, since its modularity, control reusability, flexibility and other valuable inherited characteristics from the proposed High-Level Petri Nets.
[Cut-off points of fasting fingertip capillary blood glucose for detecting both undiagnosed diabetes and pre-diabetes]. (2010)

Fasting capillary blood glucose with the lower cut-point of 6.0 mmol/L in screening for undiagnosed diabetes mellitus alone, was relatively reliable, whereas for both IFG and IGT the fasting fingertip blood glucose tests were fallible. It was convenient and could be used in screening the DM at the community level.
Large-Scale Clinical Expansion of Mesenchymal Stem Cells in the GMP-Compliant, Closed Automated Quantum® Cell Expansion System: Comparison with Expansion in Traditional T-Flasks (2014)

peer reviewed
Head and Neck Cutaneous Squamous Cell Carcinoma Requiring Parotidectomy: Prognostic Indicators and Treatment Selection (2014)

In this study, cervical, but not parotid, lymph node involvement was associated with poor outcomes in patients with advanced cSCC requiring a parotidectomy. In patients without evidence of cervical or parotid lymph node involvement, a neck dissection may be spared, given there is a 5% chance of occult disease.
Implications of bleeding and blood transfusion in percutaneous coronary intervention. (2007)

For patients undergoing percutaneous coronary intervention (PCI), bleeding complications are a major clinical concern. With advances in pharmacotherapy and devices over the past 2 decades, the risk of ischemic outcomes, such as myocardial infarction or death, has decreased. Bleeding complications have more recently become a clinical and research priority. Determining the incidence of and risk factors for bleeding is complicated by the multiple systems used to classify bleeding severity and report bleeding events. The origin of the data, clinical trials versus registries, also influences the incidence of reported bleeding events. Registry data suggest that risk of bleeding among patients undergoing PCI is higher in clinical practice than the incidence observed in clinical trials. Another clinical concern is the possible association between PCI-related bleeding complications and myocardial infarction, stroke, or death. Reduction in bleeding risk is a desirable goal that may potentially improve survival and increase comfort for patients undergoing PCI. Using strategies such as careful vascular access, alternative radial artery access, and modified antithrombotic regimen may reduce bleeding during PCI as well as improve patient outcomes.
Advertising reach and frequency: maximizing advertising results through effective frequency (1996)

An expanded edition (first, 1979) by a new author provides overview and analysis of the concepts of advertising reach and frequency with reference to advertising budgeting, timing, media selection, measurement, and related topics. Annotation copyright Book News, Inc. Portland, Or.
Consensus Statement on Continuous EEG in Critically Ill Adults and Children, Part II (2015)

Recommended qualifications for CCEEG personnel and CCEEG technical specifications will facilitate standardization of this emerging technology.
ERGOTOCIN: THE ACTIVE PRINCIPLE OF ERGOT RESPONSIBLE FOR THE ORAL EFFECTIVENESS OF SOME ERGOT PREPARATIONS ON HUMAN UTERI (1935)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTERGOTOCIN: THE ACTIVE PRINCIPLE OF ERGOT RESPONSIBLE FOR THE ORAL EFFECTIVENESS OF SOME ERGOT PREPARATIONS ON HUMAN UTERIM. S. Kharasch and R. R. LegaultCite this: J. Am. Chem. Soc. 1935, 57, 5, 956–957Publication Date (Print):May 1, 1935Publication History Published online1 May 2002Published inissue 1 May 1935https://doi.org/10.1021/ja01308a505Request reuse permissionsArticle Views27Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (233 KB) Get e-Alertsclose Get e-Alerts
Flexure and pressure-loading effects on the performance of structure–battery composite beams (2018)

The effects of sustained three-point bend loading and hydrostatic pressure on the mechanical and energy-storage performance of three structure–battery beam prototypes were experimentally investigated. The SB beams, designed for unmanned underwater vehicle applications, were fabricated using marine-grade structural composite constituents and commercial rechargeable lithium-ion “pouch” cells. Low-temperature cure materials and multistep processing were used in fabrication to avoid exposing the cells to temperatures above 60℃. The results showed load relaxation (up to 6–18%) under constant displacement three-point bending within the elastic regime due to viscoelastic shear in adhesive bond layers between components and lamina. Concurrent cell charge–discharge during sustained load bending had a small effect on the load (∼1% change or less). Energy storage capacity under hydrostatic pressures up to 2 MPa, equivalent to 200 m ocean depth, showed a 6–8% decrease in capacity. The results highlighted the need for some design changes to improve structure–battery component performance including: exclusive use of high-temperature cure resins (epoxy or vinyl ester) to improve structural performance and enable single-step fabrication, and transverse (fiber) reinforcement to strengthen the interlayer bonds and embedded cell pockets to minimize load relaxation effects and maximize component bending strength.
Red blood cells mediate the onset of thrombosis in the ferric chloride murine model (2013)

Application of ferric chloride (FeCl(3)) to exposed blood vessels is widely used to initiate thrombosis in laboratory mice. Because the mechanisms by which FeCl(3) induces endothelial injury and subsequent thrombus formation are little understood, we used scanning electron and brightfield intravital microscopy to visualize endothelial damage and thrombus formation occurring in situ. Contrary to generally accepted belief, FeCl(3) does not result in appreciable subendothelial exposure within the time frame of thrombosis. Furthermore, the first cells to adhere to FeCl(3)-treated endothelial surfaces are red blood cells (RBCs) rather than platelets. Energy dispersive x-ray spectroscopy demonstrated that ferric ions predominantly localize to endothelial-associated RBCs and RBC-derived structures rather than to the endothelium. With continuing time points, RBC-derived structures rapidly recruit platelets, resulting in large complexes that subsequently enlarge and coalesce, quickly covering the endothelial surface. Further studies demonstrated that neither von Willebrand factor nor platelet glycoprotein Ib-α receptor (GPIb-α) is required for RBCs to adhere to the endothelium, and that deficiency of GPIb-α greatly abrogated the recruitment of platelets to the endothelial-associated RBC material. These findings illuminate the mechanisms of FeCl(3)-mediated thrombosis and reveal a previously unrecognized ability of RBCs to participate in thrombosis by mediating platelet adhesion to the intact endothelial surface.
Normalization of blood glucose in diabetic rats with phlorizin treatment reverses insulin-resistant glucose transport in adipose cells without restoring glucose transporter gene expression. (1991)

Evidence is emerging for a direct role of glucose, independent of changes in insulin, in the regulation of cellular glucose transport and glucose utilization in vivo. In this study we investigate potential cellular and molecular mechanisms for this regulatory effect of glucose by determining how normalization of glycemia without insulin therapy in diabetic rats influences 3-O-methylglucose transport and the expression and translocation of two genetically distinct species of glucose transporters (GTs) in adipose cells. These results are compared with alterations in glucose disposal in vivo measured by euglycemic clamp. In rats rendered diabetic by 90% pancreatectomy, insulin-stimulated glucose transport in adipose cells is decreased 50% in parallel with reduced insulin-mediated glucose disposal in vivo. Levels of adipose/muscle GTs measured by immunoblotting are decreased in adipose cell subcellular membrane fractions, as are the corresponding mRNA levels assessed by Northern blotting of total adipose cell RNA. Normalization of blood glucose in diabetic rats with phlorizin, which impairs renal tubular glucose reabsorption and thus enhances glucose excretion, restores insulin-stimulated glucose transport in adipose cells and insulin-mediated glucose disposal in vivo. Importantly, levels of the adipose/muscle GT protein remain 43% reduced in the low-density microsomes in the basal state and 46% reduced in the plasma membranes in the insulin-stimulated state. Adipose/muscle GT mRNA levels remain approximately 50% depressed. Levels of the HepG2/brain GT protein and mRNA are unaltered by diabetes or phlorizin treatment. Thus, changes in ambient glucose independent of changes in ambient insulin can regulate the glucose transport response to insulin in isolated adipose cells and changes in responsiveness parallel alterations in glucose uptake in vivo. Since this effect can occur without alteration in the expression of the two species of glucose transporters present in adipose cells or in their translocation to the plasma membrane in response to insulin, it may result from changes in GT functional activity.
Wavelength-Selective Coupling and Decoupling of Polymer Chains via Reversible [2 + 2] Photocycloaddition of Styrylpyrene for Construction of Cytocompatible Photodynamic Hydrogels (2018)

Reversible photocycloaddition reactions have previously been employed in chemical cross-linking for the preparation of biomaterial scaffolds. However, the processes require activation by high-energy UV light, rendering them unsuitable for modification in biological environments. Here we demonstrate that the [2 + 2] photocycloaddition of styrylpyrene can be activated by visible light at λ = 400-500 nm, enabling rapid and effective conjugation and cross-linking of poly(ethylene glycol) (PEG) in water and under mild irradiation conditions (<i>I</i> = 20 mW cm<sup>-2</sup>). Notably, the reversion of the cycloaddition can be triggered by low-energy UV light at λ = 340 nm, which allows for efficient cleavage of the dimer adduct. Using this wavelength-gated reversible photochemical reaction we are able to prepare PEG hydrogels and modulate their mechanical properties in a bidirectional manner. We also demonstrate healing of the fractured hydrogel by external light triggers. Furthermore, we show that human mesenchymal stem cells can be encapsulated within the gels with high viability post encapsulation. This photochemical approach is therefore anticipated to be highly useful in studies of cell mechanotransduction, with relevance to disease progression and tissue regeneration.
Simultaneous modification of pyrolysis and densification for low-temperature solution-processed flexible oxide thin-film transistors (2012)

High-pressure annealing (HPA) affected the thermodynamics of the formation of a solution-processed oxide film through the simultaneous modification of thermal decomposition and compression, and enabled the use of lower annealing temperatures, which was favourable for device implementation. HPA also reduced the film thickness and decreased the porosity, resulting in enhanced device characteristics at low temperature. Surface and depth profile characterization using X-ray reflectivity (XRR), X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (TOF-SIMS), and ellipsometry suggested that the HPA process supported the effective decomposition of commercial metal-nitrate and/or -salt precursors and strong bonding between oxygen and the metal ions, ultimately reducing the amount of organic residue. The as-optimized HPA process allowed for high-performance solution-processed flexible InZnO (IZO) TFTs on a polymeric substrate at 220 °C with low sub-threshold voltage swing (as low as 0.56 V dec−1), high on–off ratio of over 106, and field-effect mobility as high as 1.78 cm2 V−1 s−1, respectively. These results demonstrate that this is a simple and efficient promising approach for improving the performance of solution-processed electronic devices at low temperatures.
Kirigami/Origami‐Based Soft Deployable Reflector for Optical Beam Steering (2017)

The beam steering mechanism has been a key element for various applications ranging from sensing and imaging to solar tracking systems. However, conventional beam steering systems are bulky and complex and present significant challenges for scaling up. This work introduces the use of soft deployable reflectors combining a soft deployable structure with simple kirigami/origami reflective films. This structure can be used as a macroscale beam steering mechanism that is both simple and compact. This work first develops a soft deployable structure that is easily scalable by patterning of soft linear actuators. This soft deployable structure is capable of increasing its height several folds by expanding in a continuous and controllable manner, which can be used as a frame to deform the linearly stretchable kirigami/origami structures integrated into the structure. Experiments on the reflective capability of the reflectors are conducted and show a good fit to the modeling results. The proposed principles for deployment and for beam steering can be used to realize novel active beam steering devices, highlighting the use of soft robotic principles to produce scalable morphing structures.
Mannose 6-Phosphate/Insulin-like Growth Factor II Receptor Mediates Internalization and Degradation of Leukemia Inhibitory Factor but Not Signal Transduction (1999)

Leukemia inhibitory factor (LIF) is a multifunctional cytokine belonging to the interleukin-6 subfamily of helical cytokines, all of which use the glycoprotein (gp) 130 subunit for signal transduction. The specific receptor for LIF, gp190, binds this cytokine with low affinity and is also required for signal transduction. We have recently reported that glycosylated LIF produced by transfected Chinese hamster ovary cells also binds to a lectin-like receptor, mannose 6-phosphate/insulin-like growth factor II receptor (Man-6-P/IGFII-R) (Blanchard, F., Raher, S., Duplomb, L., Vusio, P., Pitard, V., Taupin, J. L., Moreau, J. F., Hoflack, B., Minvielle, S., Jacques, Y., and Godard, A. (1998) J. Biol. Chem. 273, 20886-20893). The present study shows that (i) mannose 6-phosphate-containing LIF is naturally produced by a number of normal and tumor cell lines; (ii) other cytokines in the interleukin-6 family do not bind to Man-6-P/IGFII-R; and (iii) another unrelated cytokine, macrophage-colony-stimulating factor, is also able to bind to Man-6-P/IGFII-R in a mannose 6-phosphate-sensitive manner. No functional effects or signal transductions mediated by this lectin-like receptor were observed in various biological assays after LIF binding, and mannose 6-phosphate-containing LIF was as active as non-glycosylated LIF. However, mannose 6-phosphate-sensitive LIF binding resulted in rapid internalization and degradation of the cytokine on numerous cell lines, which suggests that Man-6-P/IGFII-R plays an important role in regulating the amounts of LIF available in vivo.