gebhart/patrepeval huggingface dataset:
Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents. (1976)

Twenty-three soluble (nonparticulate) radioactive agents were screened for their ability to label leukocytes in vitro in the presence or absence of plasma and red cells. The degree of cell binding was compared with that of other agents previously reported. Leukocyte binding of 2% or more of the added radioactivity was obtained with ten of these agents. High labeling yields were obtained only with nonpolar lipid-soluble complexes capable of penetrating lipid cell membranes. Unfortunately, this type of labeling tends to be reduced by plasma protein. Some lipophilic radioactive agents tend to elute from the cells after labeling. Compared with radioactive particles, soluble radiopharmaceuticals offer the advantage of easy centrifugal separation of the cell-bound from the residual activity in the suspension fluid. However, the labeling yield tends to be lower than for radioactive particles, and other cellular elements are labeled in addition to the phagocytic leukocytes. In this survey, the most promising soluble agents were 111In or 99mTc complexes of oxine, 111In-tetraphenylporphyrin, DNA labeled with radioactive iodine, and DNA labeled with 77Br and complexed with adriamycin. The ultimate value of any of these agents must await adequate in vivo testing in experimental animals and clinical trials.
Size-controlled growth of colloidal gold nanoplates and their high-purity acquisition (2010)

A facile while flexible approach to size-controllable high-purity colloidal gold nanoplates has been presented. By adjusting the quantity of seeds and I(-) through a seed-mediated, cetyltrimethylammonium bromide (CTABr)-assisted synthetic system, the edge length of the gold nanoplates can be adjusted from 140 to 30 nm without changing their thickness or crystal structure. By simply increasing the ion concentration of the reaction solution, the as-prepared gold nanoplates can be deposited due to the different electrostatic aggregation effects between nanoplates and spherical nanoparticles. Effective storage methods to keep the structural and optical stability of these gold nanoplates are also proposed.
Cross-talk Characteristics Of Af3 Phase Reversal Directional Coupler Switches (1985)

Cross-talk characteristics of optical waveguide directional coupler switches using two-sections of alternating Δβ phase mismatches are studied. In particular, it is found that the cross-talk characteristics can be influenced by the presence of passive coupling between the waveguides outside the electrode-interaction region, by the use of unequal voltages applied to each section and by the use of unequal sectional electrode lengths. The switching diagrams showing the contour maps of the regions corresponding to differing crosstalk levels for each case are presented.
Liquid contact resonance AFM: analytical models, experiments, and limitations (2014)

Contact resonance AFM (CR-AFM) is a scanning probe microscopy technique that utilizes the contact resonances of the AFM cantilever for concurrent imaging of topography and surface stiffness. The technique has not been used in liquid until recently due to analytical and experimental difficulties, associated with viscous damping of cantilever vibrations and fluid loading effects. To address these difficulties, (i) an analytical approach for contact resonances in liquid is developed, and (ii) direct excitation of the contact resonances is demonstrated by actuating the cantilever directly in a magnetic field. By implementing the analytical approach and the direct actuation through magnetic particles, quantitative stiffness imaging on surfaces with a wide range of stiffness can be achieved in liquid with soft cantilevers and low contact forces.
α<sub>E</sub>β<sub>7</sub> and α<sub>4</sub>β<sub>7</sub> integrins associated with intraepithelial and mucosal homing, are expressed on macrophages (1995)

The two beta 7 integrins alpha E beta 7 and alpha 4 beta 7 are the most recently described members of the integrins participating in intercellular binding. Their expression has been shown to be restricted to leukocytes and they have been suggested to be predominantly found in lymphocytes associating with the epithelium. Expression of beta 7 has mainly been studied on lymphocytes whereas macrophages have been reported not to express the beta 7 integrins. In this paper we have studied the expression of beta 7 integrins in monocytoid cells. The myelomonocytic cell lines HL-60 and THP-1 did not express beta 7 mRNA or protein, but differentiation of these cell lines to macrophages with phorbol 12-myristate 13-acetate (PMA) led to a strong induction of the beta 7 mRNA expression. A clear but less pronounced up-regulation of beta 7 mRNA-expression was also seen after treatment of HL-60 and THP-1 cells with interferon-gamma (IFN-gamma). However, its up-regulating effect on the surface expression of alpha 4 beta 7 and alpha E beta 7 complexes (detected by the monoclonal antibodies Act I and HML-1, respectively) exceeded that observed with PMA. To verify the in vitro cell line observations with normal cells, we also studied peripheral blood monocytes and tissue macrophages. Peripheral blood monocytes were Act I- and HML-1- in flow cytometry, but their expression was increased after a 72-h culture in the presence of PMA or IFN-gamma. Also, several Act I+ and HML-1+ macrophages were found in immunohistochemical stainings of both liver and edemic lung biopsies as well as in lymph node sinuses. We therefore conclude that while monocytes do not express beta 7 integrins the more differentiated cells of the monocyte-macrophage lineage do express both the alpha 4 beta 7 and alpha E beta 7 integrins, which might play a role in their intraepithelial homing.
Non-invasive assessment of barrier integrity and function of the human gut (2010)

Over the past decades evidence has been accumulating that intestinal barrier integrity loss plays a key role in the development and perpetuation of a variety of disease states including inflammatory bowel disease and celiac disease, and is a key player in the onset of sepsis and multiple organ failure in situations of intestinal hypoperfusion, including trauma and major surgery. Insight into gut barrier integrity and function loss is important to improve our knowledge on disease etiology and pathophysiology and contributes to early detection and/or secondary prevention of disease. A variety of tests have been developed to assess intestinal epithelial cell damage, intestinal tight junction status and consequences of intestinal barrier integrity loss, i.e. increased intestinal permeability. This review discusses currently available methods for evaluating loss of human intestinal barrier integrity and function.
A Computational Model of Embodied Language Learning (2003)

Language is about symbols and those symbols must be grounded in the physical environment during human development. Most recently, there has been an increased awareness of the essential role of inferences of speakers&apos; referential intentions in grounding those symbols. Experiments have shown that these inferences as revealed in eye, head and hand movements serve as an important driving force in language learning at a relatively early age. The challenge ahead is to develop formal models of language acquisition that can shed light on the leverage provided by embodiment. We present an implemented computational model of embodied language acquisition that learns words from natural interactions with users. The system can be trained in unsupervised mode in which users perform everyday tasks while providing natural language descriptions of their behaviors. We collect acoustic signals in concert with user-centric multisensory information from nonspeech modalities, such as user&apos;s perspective video, gaze positions, head directions and hand movements. A multimodal learning algorithm is developed that firstly spots words from continuous speech and then associates action verbs and object names with their grounded meanings. The central idea is to make use of non-speech contextual information to facilitate word spotting, and utilize user&apos;s attention as deictic reference to discover temporal correlations of data from different modalities to build lexical items. We report the results of a series of experiments that demonstrate the effectiveness of our approach.
Organic Dyes Incorporating the Benzo[1,2-<i>b</i>:4,5-<i>b</i>′]dithiophene Moiety for Efficient Dye-Sensitized Solar Cells (2011)

A new class of organic sensitizers incorporating a benzo[1,2-b:4,5-b']dithiophene (BDT) unit as conjugated spacer has been synthesized and successfully used for dye-sensitized solar cells (DSSCs). The length of the π-conjugated spacers has a strong impact on electro-optical properties of these dyes, leading to the conversion efficiencies ranging from 4.17 to 5.68% under AM 1.5 G irradiation. This result indicates that the BDT unit is a promising candidate in organic sensitizers.
Establishment of persistent infection with non-cytopathic bovine viral diarrhoea virus in cattle is associated with a failure to induce type I interferon (2001)

The establishment of persistent infections with non-cytopathic bovine virus diarrhoea virus (ncpBVDV) is crucial for the maintenance of BVDV in cattle populations. Also, super-infection of persistently infected individuals with antigenically homologous cytopathic BVDV (cpBVDV) results in fatal mucosal disease. Persistent infection with ncpBVDV is established by infection of the foetus during the first trimester of pregnancy. It has been shown previously that foetal infection with cpBVDV does not result in persistent infection. Infection of cells in vitro has demonstrated that cpBVDV induces type I interferon (IFN), whereas ncpBVDV fails to induce IFN. In this study we demonstrate that foetal challenge with cpBVDV results in IFN production, whereas ncpBVDV does not. These findings strongly suggest that the ability of ncpBVDV to inhibit the induction of type I IFN has evolved to enable the virus to establish persistent infection in the early foetus.
Defining Binding Efficiency and Specificity of Auxins for SCF<sup>TIR1/AFB</sup>-Aux/IAA Co-receptor Complex Formation (2013)

Structure-activity profiles for the phytohormone auxin have been collected for over 70 years, and a number of synthetic auxins are used in agriculture. Auxin classification schemes and binding models followed from understanding auxin structures. However, all of the data came from whole plant bioassays, meaning the output was the integral of many different processes. The discovery of Transport Inhibitor-Response 1 (TIR1) and the Auxin F-Box (AFB) proteins as sites of auxin perception and the role of auxin as molecular glue in the assembly of co-receptor complexes has allowed the development of a definitive quantitative structure-activity relationship for TIR1 and AFB5. Factorial analysis of binding activities offered two uncorrelated factors associated with binding efficiency and binding selectivity. The six maximum-likelihood estimators of Efficiency are changes in the overlap matrixes, inferring that Efficiency is related to the volume of the electronic system. Using the subset of compounds that bound strongly, chemometric analyses based on quantum chemical calculations and similarity and self-similarity indices yielded three classes of Specificity that relate to differential binding. Specificity may not be defined by any one specific atom or position and is influenced by coulomb matrixes, suggesting that it is driven by electrostatic forces. These analyses give the first receptor-specific classification of auxins and indicate that AFB5 is the preferred site for a number of auxinic herbicides by allowing interactions with analogues having van der Waals surfaces larger than that of indole-3-acetic acid. The quality factors are also examined in terms of long-standing models for the mechanism of auxin binding.
Spectral Line Elimination and Spontaneous Emission Cancellation via Quantum Interference (1996)

Quantum interference in spontaneous emission from a four-level atom is investigated. The atom has two upper levels coupled by the same vacuum modes to a common lower level and is driven by a coherent field to an auxiliary level. Interference can lead to the elimination of a spectral line in the spontaneous emission spectrum and spontaneous emission cancellation in steady state.
Improved vocal tract reconstruction and modeling using an image super-resolution technique (2013)

Magnetic resonance imaging has been widely used in speech production research. Often only one image stack (sagittal, axial, or coronal) is used for vocal tract modeling. As a result, complementary information from other available stacks is not utilized. To overcome this, a recently developed super-resolution technique was applied to integrate three orthogonal low-resolution stacks into one isotropic volume. The results on vowels show that the super-resolution volume produces better vocal tract visualization than any of the low-resolution stacks. Its derived area functions generally produce formant predictions closer to the ground truth, particularly for those formants sensitive to area perturbations at constrictions.
Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome (2016)

The yeast 2-micron plasmid epitomizes the evolutionary optimization of selfish extra-chromosomal genomes for stable persistence without jeopardizing their hosts' fitness. Analyses of fluorescence-tagged single-copy reporter plasmids and/or the plasmid partitioning proteins in native and non-native hosts reveal chromosome-hitchhiking as the likely means for plasmid segregation. The contribution of the partitioning system to equal segregation is bipartite- replication-independent and replication-dependent. The former nearly eliminates 'mother bias' (preferential plasmid retention in the mother cell) according to binomial distribution, thus limiting equal segregation of a plasmid pair to 50%. The latter enhances equal segregation of plasmid sisters beyond this level, elevating the plasmid close to chromosome status. Host factors involved in plasmid partitioning can be functionally separated by their participation in the replication-independent and/or replication-dependent steps. In the hitchhiking model, random tethering of a pair of plasmids to chromosomes signifies the replication-independent component of segregation; the symmetric tethering of plasmid sisters to sister chromatids embodies the replication-dependent component. The 2-micron circle broadly resembles the episomes of certain mammalian viruses in its chromosome-associated propagation. This unifying feature among otherwise widely differing selfish genomes suggests their evolutionary convergence to the common logic of exploiting, albeit via distinct molecular mechanisms, host chromosome segregation machineries for self-preservation.
Phase‐field boundary conditions for the voxel finite cell method: Surface‐free stress analysis of CT‐based bone structures (2017)

The voxel finite cell method uses unfitted finite element meshes and voxel quadrature rules to seamlessly transfer computed tomography data into patient-specific bone discretizations. The method, however, still requires the explicit parametrization of boundary surfaces to impose traction and displacement boundary conditions, which constitutes a potential roadblock to automation. We explore a phase-field-based formulation for imposing traction and displacement constraints in a diffuse sense. Its essential component is a diffuse geometry model generated from metastable phase-field solutions of the Allen-Cahn problem that assumes the imaging data as initial condition. Phase-field approximations of the boundary and its gradient are then used to transfer all boundary terms in the variational formulation into volumetric terms. We show that in the context of the voxel finite cell method, diffuse boundary conditions achieve the same accuracy as boundary conditions defined over explicit sharp surfaces, if the inherent length scales, ie, the interface width of the phase field, the voxel spacing, and the mesh size, are properly related. We demonstrate the flexibility of the new method by analyzing stresses in a human femur and a vertebral body.
Molecular-timetable methods for detection of body time and rhythm disorders from single-time-point genome-wide expression profiles (2004)

Detection of individual body time (BT) via a single-time-point assay has been a longstanding unfulfilled dream in medicine, because BT information can be exploited to maximize potency and minimize toxicity during drug administration and thus will enable highly optimized medication. To achieve this dream, we created a "molecular timetable" composed of >100 "time-indicating genes," whose gene expression levels can represent internal BT. Here we describe a robust method called the "molecular-timetable method" for BT detection from a single-time-point expression profile. The power of this method is demonstrated by the sensitive and accurate detection of BT and the sensitive diagnosis of rhythm disorders. These results demonstrate the feasibility of BT detection based on single-time-point sampling, suggest the potential for expression-based diagnosis of rhythm disorders, and may translate functional genomics into chronotherapy and personalized medicine.
Varvighed i danske og skaanske senglaciale Aflejringer. Med særlig Hensyntagen til Egernsund Issøsystemet. Atlas (1940)

Atlas med 45 tavler og 2 kort.
Cyclic AMP increases the survival of ganglion cells in mixed retinal cell cultures in the absence of exogenous neurotrophic molecules, an effect that involves cholinergic activity (2001)

Natural cell death is a well-known degenerative phenomenon occurring during development of the nervous system. The role of trophic molecules produced by target and afferent cells as well as by glial cells has been extensively demonstrated. Literature data demonstrate that cAMP can modulate the survival of neuronal cells. Cultures of mixed retinal cells were treated with forskolin (an activator of the enzyme adenylyl cyclase) for 48 h. The results show that 50 microM forskolin induced a two-fold increase in the survival of retinal ganglion cells (RGCs) in the absence of exogenous trophic factors. This effect was dose dependent and abolished by 1 microM H89 (an inhibitor of protein kinase A), 1.25 microM chelerythrine chloride (an inhibitor of protein kinase C), 50 microM PD 98059 (an inhibitor of MEK), 25 microM Ly 294002 (an inhibitor of phosphatidylinositol-3 kinase), 30 nM brefeldin A (an inhibitor of polypeptide release), and 10 microM genistein or 1 ng/ml herbimycin (inhibitors of tyrosine kinase enzymes). The inhibition of muscarinic receptors by 10 microM atropine or 1 microM telenzepine also blocked the effect of forskolin. When we used 25 microM BAPTA, an intracellular calcium chelator, as well as 20 microM 5-fluoro-2'-deoxyuridine, an inhibitor of cell proliferation, we also abolished the effect. Our results indicate that cAMP plays an important role controlling the survival of RGCs. This effect is directly dependent on M1 receptor activation indicating that cholinergic activity mediates the increase in RGC survival. We propose a model which involves cholinergic amacrine cells and glial cells in the increase of RGC survival elicited by forskolin treatment.
A space-frequency-time diversity scheme for MIMO-OFDM systems (2006)

In this paper, a new coding scheme, for MIMO-OFDM systems that tends to exploit both space, time, and frequency diversity is introduced. The code, partially based on the well-known Alamouti scheme, works with a configuration of two transmit and n/sub r/ receive antennas and requires a channel with coherent impulse response during two OFDM signaling intervals. Monte Carlo simulations have been run to compare the new scheme with other designs. Simulations have shown that this scheme performs better than direct MIMO-OFDM transposition of space-time codes (e.g. transpositions of Alamouti scheme). Despite the reduced code rate 1/2 of this scheme, its performances show a superior diversity order characterized by a steeper slope of the bit error rate curves. Simulations were also run to gauge the effects of errors on channel parameters estimation. These errors were simulated through, a model derived from a MIMO-OFDM channel estimation technique.
Organocatalyzed Decarboxylation of Naturally Occurring Cinnamic Acids: Potential Role in Flavoring Chemicals Production (2011)

The mechanism and the final outcome of the Knoevenagel-Doebner reaction are discussed. The condensation reaction between different hydroxy-substituted aromatic aldehydes and malonic acid is performed using piperidine as organocatalyst. The key role of the catalyst is clearly pointed out during the decarboxylation of ferulic acid, without the use of a strong decarboxylating agent, leading to a 4-vinylphenol derivative. Based on the results obtained, the studied pathway may be important in the understanding of vinylphenol production during malting and brewing of wheat and barley grains. Finally, changing the solvent of the reaction from pyridine to water in the Knoevenagel-Doebner reaction of 4-hydroxybenzaldehydes, dimerization of resulting styrene derivatives is observed. These results can be of interest also in the field of food chemistry, since cinnamic acids are frequently found in fruits and vegetables used for human consumption.
Characterization of the lipopolysaccharide and β‐glucan of the fish pathogen <i>Francisella victoria</i> (2006)

Lipopolysaccharide (LPS) and beta-glucan from Francisella victoria, a fish pathogen and close relative of highly virulent mammal pathogen Francisella tularensis, have been analyzed using chemical and spectroscopy methods. The polysaccharide part of the LPS was found to contain a nonrepetitive sequence of 20 monosaccharides as well as alanine, 3-aminobutyric acid, and a novel branched amino acid, thus confirming F. victoria as a unique species. The structure identified composes the largest oligosaccharide elucidated by NMR so far, and was possible to solve using high field NMR with cold probe technology combined with the latest pulse sequences, including the first application of H2BC sequence to oligosaccharides. The non-phosphorylated lipid A region of the LPS was identical to that of other Francisellae, although one of the lipid A components has not been found in Francisella novicida. The heptoseless core-lipid A region of the LPS contained a linear pentasaccharide fragment identical to the corresponding part of F. tularensis and F. novicida LPSs, differing in side-chain substituents. The linkage region of the O-chain also closely resembled that of other Francisella. LPS preparation contained two characteristic glucans, previously observed as components of LPS preparations from other strains of Francisella: amylose and the unusual beta-(1-6)-glucan with (glycerol)2phosphate at the reducing end.
New acoustic resonator for liquids in the 0.2− to 2−MHz range (1975)

A new acoustic resonator with nonplanar quartz transducers for liquids (sample volume about 20 ml) in the 0.2− to 2−MHz range is described. The application of static overpressure onto the liquid cavity causes concave bending of both quartz transducers. This reduces loss contributions from diffraction and ’’side−wall effects’’ considerably, resulting in higher Q values for this resonator; moreover, air inclusions in the cavity are suppressed. Subject Classification: 35.24, 35.80.