gebhart/patrepeval huggingface dataset:
Speciation without chromatography : Part I. Determination of tributyltin in aqueous samples by chloride generation, headspace solid-phase microextraction and inductively coupled plasma time of flight mass spectrometry (2001)

An analytical procedure was developed for the determination of tributyltin in aqueous samples. The relatively high volatility of the organometal halide species confers suitability for their headspace sampling from the vapour phase above natural waters or leached solid samples. Tributyltin was collected from the sample headspace above various chloride-containing matrices, including HCl, sodium chloride solution and sea-water, by passive sampling using a polydimethylsiloxane/divinylbenzene (PDMS/DVB)-coated solid-phase microextraction (SPME) fiber. Inductively coupled plasma time-of-flight mass spectrometry (ICP-TOFMS) was used for detection following thermal desorption of analytes from the fiber. A detection limit of 5.8 pg ml−1 (as tin) was realized in aqueous samples. Method validation was achieved using NRCC PACS-2 (Sediment) certified reference material, for which reasonable agreement between certified and measured values for tributyltin content was obtained.
Cermets: II, Wettability and Microstructure Studies in Liquid‐Phase Sintering (1957)

The microstructures of polyphase, heterogeneous systems, sintered in the presence of a liquid phase, are analyzed on the basis of wettability and surface‐energy relations. General principles relating to the primary factors affecting the microstructure are discussed. The application of these principles to such diverse systems as metal‐carbide, metal‐glass, and metal‐metal show that the microstructure and distribution of the solid in the liquid is primarily determined by various surface‐energy relationships. A general “coalescence hypothesis” based on this work is proposed.
CNN Features Off-the-Shelf: An Astounding Baseline for Recognition (2014)

Recent results indicate that the generic descriptors extracted from the convolutional neural networks are very powerful. This paper adds to the mounting evidence that this is indeed the case. We report on a series of experiments conducted for different recognition tasks using the publicly available code and model of the OverFeat network which was trained to perform object classification on ILSVRC13. We use features extracted from the OverFeat network as a generic image representation to tackle the diverse range of recognition tasks of object image classification, scene recognition, fine grained recognition, attribute detection and image retrieval applied to a diverse set of datasets. We selected these tasks and datasets as they gradually move further away from the original task and data the OverFeat network was trained to solve. Astonishingly, we report consistent superior results compared to the highly tuned state-of-the-art systems in all the visual classification tasks on various datasets. For instance retrieval it consistently outperforms low memory footprint methods except for sculptures dataset. The results are achieved using a linear SVM classifier (or L2 distance in case of retrieval) applied to a feature representation of size 4096 extracted from a layer in the net. The representations are further modified using simple augmentation techniques e.g. jittering. The results strongly suggest that features obtained from deep learning with convolutional nets should be the primary candidate in most visual recognition tasks.
Large-Scale Identification of Virulence Genes from <i>Streptococcus pneumoniae</i> (1998)

Streptococcus pneumoniae is the major cause of bacterial pneumonia, and it is also responsible for otitis media and meningitis in children. Apart from the capsule, the virulence factors of this pathogen are not completely understood. Recent technical advances in the field of bacterial pathogenesis (in vivo expression technology and signature-tagged mutagenesis [STM]) have allowed a large-scale identification of virulence genes. We have adapted to S. pneumoniae the STM technique, originally used for the discovery of Salmonella genes involved in pathogenicity. A library of pneumococcal chromosomal fragments (400 to 600 bp) was constructed in a suicide plasmid vector carrying unique DNA sequence tags and a chloramphenicol resistance marker. The recent clinical isolate G54 was transformed with this library. Chloramphenicol-resistant mutants were obtained by homologous recombination, resulting in genes inactivated by insertion of the suicide vector carrying a unique tag. In a mouse pneumonia model, 1.250 candidate clones were screened; 200 of these were not recovered from the lungs were therefore considered virulence-attenuated mutants. The regions flanking the chloramphenicol gene of the attenuated mutants were amplified by inverse PCR and sequenced. The sequence analysis showed that the 200 mutants had insertions in 126 different genes that could be grouped in six classes: (i) known pneumococcal virulence genes; (ii) genes involved in metabolic pathways; (iii) genes encoding proteases; (iv) genes coding for ATP binding cassette transporters; (v) genes encoding proteins involved in DNA recombination/repair; and (vi) DNA sequences that showed similarity to hypothetical genes with unknown function. To evaluate the virulence attenuation for each mutant, all 126 clones were individually analyzed in a mouse septicemia model. Not all mutants selected in the pneumonia model were confirmed in septicemia, thus indicating the existence of virulence factors specific for pneumonia.
Gas-liquid chromatographic determination of solution properties of oxygenated compounds in water (1969)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGas-liquid chromatographic determination of solution properties of oxygenated compounds in waterDaniel L. Shaffer and Thomas E. DaubertCite this: Anal. Chem. 1969, 41, 12, 1585–1589Publication Date (Print):October 1, 1969Publication History Published online1 May 2002Published inissue 1 October 1969https://pubs.acs.org/doi/10.1021/ac60281a016https://doi.org/10.1021/ac60281a016research-articleACS PublicationsRequest reuse permissionsArticle Views92Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Glass Transition and Infrared Spectra of Low‐Alkali, Anhydrous Lithium Phosphate Glasses (1993)

Anhydrous glasses in the series x Li 2 O + (1 ‐ x )P 2 O 5 have been prepared and characterized in the range 0 ≤ x ≤ 0.5. FT‐IR spectroscopy and glass transition temperature measurements have been used to explore the structure and a key physical property of the low‐alkali phosphate glasses. The structure of v ‐P 2 O 5 is proposed to consist of a 3‐D network of trigonally connected tetrahedra decorated with a P=O unit. Contrary to what has long been proposed for these glasses, the addition of alkali degrades the 3‐D network through the generation of nonbridging oxygens rather than strengthen the network through the proposed alkali ion bridging. The T g of v ‐P 2 O 5 is ∼653 K and decreases some 130 K with the addition of 10 mol% Li 2 O. T g then reaches a minimum value at 20 mol% Li 2 O and increases with further Li 2 O additions. The increase in T g , even though the fraction of nonbridging oxygens is still increasing, is interpreted in terms of an increasing entanglement of long‐chain PO 2 groups in the glass. Such a structural transition from a 3‐D network of interconnected PO 4 groups for P 2 O 5 to a 1‐D chain structure for LiPO 3 is one of the first examples of the importance of intermediate‐range order in governing the properties of glass.
Biotransformation, a new approach to aminoglycoside biosynthesis. II. Gentamicin. (1976)

Utilizing a paromamine-producing mutant of Micromonospora purpurea blocked in the production of gentamicin, bioconversion of various minor gentamicin components into the gentamicin C complex was demonstrated. The compounds tested Were structurally related to the gentamicin C's and are found as minor components in the gentamicin fermentation. Based upon the bioconversions detected, a branched pathway for the biosynthesis of the gentamicin C components is proposed.
Salivary proteins associated with hyperglycemia in diabetes: a proteomic analysis (2013)

Effective monitoring of glucose levels is necessary for patients to achieve greater control over their diabetes. However, only about a quarter of subjects with diabetes who requires close serum glucose monitoring, regularly check their serum glucose daily. One of the potential barriers to patient compliance is the blood sampling requirement. Saliva and its protein contents can be altered in subjects with diabetes, possibly due to changes in glycemic control. We propose here that salivary proteomes of subjects with diabetes may be different based on their glycemic control as reflected in A1C levels. A total of 153 subjects with type 1 or 2 diabetes were recruited. Subjects in each type of diabetes were divided into 5 groups based on their A1C levels; <7, 7-8, 8-9, 9-10, >10. To examine the global proteomic changes associated with A1C, the proteomic profiling of pooled saliva samples from each group was created using label-free quantitative proteomics. Similar proteomic analysis for individual subjects (N=4, for each group) were then applied to examine proteins that may be less abundant in pooled samples. Principle component analysis (PCA) and cluster analysis (p<0.01 and p<0.001) were used to define the proteomic differences. We, therefore, defined the salivary proteomic changes associated with A1C changes. This study demonstrates that differences exist between salivary proteomic profiles in subjects with diabetes based on the A1C levels.
AnglePose (2011)

We present a finger-tracking system for touch-based interaction which can track 3D finger angle in addition to position, using low-resolution conventional capacitive sensors, therefore compensating for the inaccuracy due to pose variation in conventional touch systems. Probabilistic inference about the pose of the finger is carried out in real-time using a particle filter; this results in an efficient and robust pose estimator which also gives appropriate uncertainty estimates. We show empirically that tracking the full pose of the finger results in greater accuracy in pointing tasks with small targets than competitive techniques. Our model can detect and cope with different finger sizes and the use of either fingers or thumbs, bringing a significant potential for improvement in one-handed interaction with touch devices. In addition to the gain in accuracy we also give examples of how this technique could open up the space of novel interactions.
UEFI Firmware Storage System Analysis (2016)

The analysis of UEFI firmware storage system is rare in available papers about UEFI.We introduce the Logical and physical structure of UEFI firmware. Then we analyse the elements -firmware volume, firmware file, firmware segment --that composed the UEFI firmware and give related code define and pay additional attention to the typical firmware segment. In the experiment, we analyse a typical UEFI BIOS firmware with binary viewer and introduce the nested firmware segment.We also create a program prototype witch can resolve and extract modules included in UEFI firmware to verify the analysis correctness.
The Reaction of Sulfenyl Chlorides with Trialkyl Phosphites<sup>1</sup> (1955)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Reaction of Sulfenyl Chlorides with Trialkyl Phosphites1D. C. MorrisonCite this: J. Am. Chem. Soc. 1955, 77, 1, 181–182Publication Date (Print):January 1, 1955Publication History Published online1 May 2002Published inissue 1 January 1955https://pubs.acs.org/doi/10.1021/ja01606a062https://doi.org/10.1021/ja01606a062research-articleACS PublicationsRequest reuse permissionsArticle Views499Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of a rapid positive/absent test for coliforms using sensitive bioluminescence assay (2000)

We have developed a sensitive bioluminescence assay for beta-galactosidase using a luminescent substrate, D-luciferin-O-beta-galactopyranoside (LuGal). The detection limit for beta-galactosidase was 3 x 10-20 mol per assay, which was approximately 50-fold more sensitive than the test using a fluorescent substrate. This assay was applied to a positive/absent (P/A) test for coliforms. Observations made after 7 h of culture followed by a 10-min enzyme assay using LuGal were comparable to those made after a 22-24-h culture by the current method. Therefore, the LuGal method allows a rapid P/A test for coliforms.
Reactions of diaryl disulfides with active, nonnucleophilic alkylating agents (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of diaryl disulfides with active, nonnucleophilic alkylating agentsBernard Miller and Chung-Hwa HanCite this: J. Org. Chem. 1971, 36, 11, 1513–1519Publication Date (Print):June 1, 1971Publication History Published online1 May 2002Published inissue 1 June 1971https://pubs.acs.org/doi/10.1021/jo00810a017https://doi.org/10.1021/jo00810a017research-articleACS PublicationsRequest reuse permissionsArticle Views107Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
14-3-3 proteins are promising LRRK2 interactors (2010)

Mutations in LRRK2 (leucine-rich repeat kinase 2) are the most common cause of familial PD (Parkinson's disease). Mutations that cause PD are found in either the GTPase or kinase domains of LRRK2 or an intervening sequence called the COR [C-terminus of ROC (Ras of complex proteins)] domain. As well as the two catalytic domains, LRRK2 possesses several protein-protein interaction domains, but their function and the proteins with which they interact are poorly understood. In this issue of the Biochemical Journal, Nichols et al. study the interaction of the N-terminal region of LRRK2 with 14-3-3 proteins, regulatory proteins that often bind to phosphorylated regions of components of cell signalling pathways. Using a combination of techniques, Nichols et al. have identified two residues (Ser910 and Ser935) that are critically responsible for 14-3-3 binding. The interaction of LRRK2 with 14-3-3 proteins can prevent dephosphorylation of Ser910/Ser935 and stabilize LRRK2 structure, perhaps by influencing the dimerization of LRRK2. The ability to interact with 14-3-3 correlates with the pattern of intracellular LRRK2 distribution. Collectively, these new results identify a potentially important regulatory mechanism of this complex protein and might provide ways to think about therapeutic opportunities for PD.
Aqueous-filled polymer microcavity arrays: versatile & stable lipid bilayer platforms offering high lateral mobility to incorporated membrane proteins (2015)

A key prerequisite in an ideal supported lipid bilayer based cell membrane model is that the mobility of both the lipid matrix and its components are unhindered by the underlying support. This is not trivial and with the exception of liposomes, many of even the most advanced approaches, although accomplishing lipid mobility, fail to achieve complete mobility of incorporated membrane proteins. This is addressed in a novel platform comprising lipid bilayers assembled over buffer-filled, arrays of spherical cap microcavities formed from microsphere template polydimethoxysilane. Prior to bilayer assembly the PDMS is rendered hydrophilic by plasma treatment and the lipid bilayer prepared using Langmuir Blodgett assembly followed by liposome/proteoliposome fusion. Fluorescence Lifetime Correlation Spectroscopy confirmed the pore suspended lipid bilayer exhibits diffusion coefficients comparable to free-standing vesicles in solution. The bilayer modified arrays are highly reproducible and stable over days. As the bilayers are suspended over deep aqueous reservoirs, reconstituted membrane proteins experience an aqueous interface at both membrane interfaces and attain full lateral mobility. Their utility as membrane protein platforms was exemplified in two case studies with proteins of different dimensions in their extracellular and cytoplasmic domains reconstituted into DOPC lipid bilayers; Glycophorin A, and Integrin αIIbβ3. In both cases, the proteins exhibited 100% mobility with high lateral diffusion coefficients.
Prototype fiber-optic-based pressure probe with built-in temperature compensation with signal recovery by coherence reading (1993)

A prototype multimode fiber-based Fabry-Perot interferometric pressure probe utilizing a corrugated diaphragm with built-in temperature compensation, with signal recovery by coherence reading, has been constructed and demonstrated. A separate fiber-optic-based temperature sensor was incorporated into the pressure sensor to permit the pressure measurement to be corrected for the temperature dependence of the pressure probe. A measurement range to resolution of 3.6 × 10(4) - 1 and an overall measurement accuracy of ±0.15% have been achieved. This system represents a practical approach for industrial use.
Inhibition of Nitric Oxide Synthesis Causes Myocardial Ischemia in Endotoxemic Rats (1995)

Inhibitors of nitric oxide (NO) synthesis have been used in the treatment of septic and endotoxic shock. However, several studies question the beneficial effect of inhibiting NO production in sepsis and endotoxemia. We have investigated the effect of inhibition of NO synthesis after endotoxemia in the isolated perfused rat heart. In hearts from endotoxin-treated animals, coronary flow was elevated 64% and oxygen consumption was elevated 20% compared with control hearts. NADH fluorescence imaging was used as an indicator of regional hypoperfusion. A homogeneous low-surface NADH fluorescence, indicative of adequate tissue perfusion, was observed in both control and endotoxin-treated hearts. The increase in coronary flow and oxygen consumption could only partially be prevented by pretreatment of the animals with dexamethasone. Addition of N omega-nitro-L-arginine (NNLA), an inhibitor of NO synthesis, to the perfusion medium eliminated differences in coronary flow and oxygen consumption between normal and endotoxin-treated hearts. However, NADH surface fluorescence images of endotoxin-treated hearts after NNLA revealed areas of high fluorescence, indicating local ischemia, whereas the control hearts remained without signs of ischemia. The ischemic areas were present at various perfusion pressures and disappeared after the infusion of L-arginine, the natural precursor of NO, or the exogenous NO donor sodium nitroprusside. Methylene blue (MB), an inhibitor of soluble guanylate cyclase, the effector enzyme of NO, also eliminated differences in coronary flow and produced similar areas of local myocardial ischemia in endotoxin-treated hearts but not in control hearts.(ABSTRACT TRUNCATED AT 250 WORDS)
Identifying user behavior in online social networks (2008)

Online social networks pose an interesting problem: how to best characterize the different classes of user behavior. Traditionally, user behavior characterization methods, based on user individual features, are not appropriate for online networking sites. In these environments, users interact with the site and with other users through a series of multiple interfaces that let them to upload and view content, choose friends, rank favorite content, subscribe to users and do many other interactions. Different interaction patterns can be observed for different groups of users. In this paper, we propose a methodology for characterizing and identifying user behaviors in online social networks. First, we crawled data from YouTube and used a clustering algorithm to group users that share similar behavioral pattern. Next, we have shown that attributes that stem from the user social interactions, in contrast to attributes relative to each individual user, are good discriminators and allow the identification of relevant user behaviors. Finally, we present and discuss experimental results of the use of proposed methodology. A set of useful profiles, derived from the analysis of the YouTube sample is presented. The identification of different classes of user behavior has the potential to improve, for instance, recommendation systems for advertisements in online social networks.