gebhart/patrepeval huggingface dataset:
In vitro cytotoxicity following specific activation of amygdalin by β-glucosidase conjugated to a bladder cancer-associated monoclonal antibody (1998)

We describe a novel version of antibody-directed enzyme prodrug therapy (ADEPT), with the use of amygdalin as prodrug. Amygdalin is a naturally occurring cyanogenic glycoside, which can be cleaved by sweet almond beta-glucosidase to yield free cyanide. If amygdalin could be activated specifically at the tumour site, then malignant cells would be killed without the systemic toxicity usually associated with chemotherapy. To this end, we have conjugated beta-glucosidase to a tumour-associated monoclonal antibody (MAb) (HMFG1) and the conjugate has been tested in vitro for specificity and cytotoxicity subsequent to activation of amygdalin. Amygdalin was cytotoxic to HT1376 bladder cancer cells only at high concentrations, whereas the combination of amygdalin with HMFG1-beta-glucosidase enhanced the cytotoxic effect of amygdalin by 36-fold. When 2 concentrations of HMFG1-beta-glucosidase were compared, the toxic effect was dose dependent. The cytotoxicity of amygdalin was also enhanced by the MAb-enzyme conjugate even when the unbound conjugate was removed from the medium prior to exposure to amygdalin and the cells were washed. In addition to the cytotoxic effect, we also demonstrated specificity, using a MAb-enzyme conjugate that does not recognise the HT1376 bladder cancer cells. Finally, we studied the cytotoxic effect of the conjugate in co-culture of HMFG1-positive and-negative cell lines (HT 1376 and U87MG cells). We demonstrated that the rate of surviving cells corresponds well to the percentage of U87MG (HMFG1-negative) cells in the flask. Our findings indicate that ADEPT is more effective than non-directed enzyme activation of a prodrug and can result in a non-toxic cancer therapy.
Oral Mycobiome Analysis of HIV-Infected Patients: Identification of Pichia as an Antagonist of Opportunistic Fungi (2014)

Oral microbiota contribute to health and disease, and their disruption may influence the course of oral diseases. Here, we used pyrosequencing to characterize the oral bacteriome and mycobiome of 12 HIV-infected patients and matched 12 uninfected controls. The number of bacterial and fungal genera in individuals ranged between 8-14 and 1-9, among uninfected and HIV-infected participants, respectively. The core oral bacteriome (COB) comprised 14 genera, of which 13 were common between the two groups. In contrast, the core oral mycobiome (COM) differed between HIV-infected and uninfected individuals, with Candida being the predominant fungus in both groups. Among Candida species, C. albicans was the most common (58% in uninfected and 83% in HIV-infected participants). Furthermore, 15 and 12 bacteria-fungi pairs were correlated significantly within uninfected and HIV-infected groups, respectively. Increase in Candida colonization was associated with a concomitant decrease in the abundance of Pichia, suggesting antagonism. We found that Pichia spent medium (PSM) inhibited growth of Candida, Aspergillus and Fusarium. Moreover, Pichia cells and PSM inhibited Candida biofilms (P = .002 and .02, respectively, compared to untreated controls). The mechanism by which Pichia inhibited Candida involved nutrient limitation, and modulation of growth and virulence factors. Finally, in an experimental murine model of oral candidiasis, we demonstrated that mice treated with PSM exhibited significantly lower infection score (P = .011) and fungal burden (P = .04) compared to untreated mice. Moreover, tongues of PSM-treated mice had few hyphae and intact epithelium, while vehicle- and nystatin-treated mice exhibited extensive fungal invasion of tissue with epithelial disruption. These results showed that PSM was efficacious against oral candidiasis in vitro and in vivo. The inhibitory activity of PSM was associated with secretory protein/s. Our findings provide the first evidence of interaction among members of the oral mycobiota, and identifies a potential novel antifungal.
High-efficiency super-gain antenna arrays (2010)

It is an intriguing fact that the array gain of densely packed antenna arrays can be much larger than the number of antennas which comprise the antenna array. However, their large array gain seems to be inaccessible in practice, for it tends to be all eaten up by a loss of efficiency that accompanies such super-gain effects. In this paper, the authors argue that the assertion given above is based on a less than optimum choice of antenna separation inside the array as well as on less than optimum antenna excitation currents. We demonstrate that if both those issues are addressed optimally super-gain actually can be obtained with high efficiency. Compact antenna arrays therefore deserve to be given more attention in both the antenna-, and the signal processing layers of abstraction, for the successful application of such arrays requires optimum design in both layers.
Tessutivo (2019)

We present Tessutivo, a contact-based inductive sensing technique for contextual interactions on interactive fabrics. Our technique recognizes conductive objects (mainly metallic) that are commonly found in households and workplaces, such as keys, coins, and electronic devices. We built a prototype containing six by six spiral-shaped coils made of conductive thread, sewn onto a four-layer fabric structure. We carefully designed the coil shape parameters to maximize the sensitivity based on a new inductance approximation formula. Through a ten-participant study, we evaluated the performance of our proposed sensing technique across 27 common objects. We yielded 93.9% real-time accuracy for object recognition. We conclude by presenting several applications to demonstrate the unique interactions enabled by our technique.
Isolation and Characterization of a Full-Length Molecular DNA Clone of Ghanaian HIV Type 1 Intersubtype A/G Recombinant CRF02_AG, Which Is Replication Competent in a Restricted Host Range (2001)

We have isolated a replication-competent, full-length molecular clone of HIV-1 CRF02_AG, designated p97GH-AG1, by reconstituting two separately amplified genomic regions of an HIV-1 provirus of a 1997 Ghanaian isolate. The phylogenetic and recombination breakpoint analyses revealed that 97GH-AG1 had an A/G recombinant structure similar to that of prototype Nigerian isolate IbNG. The 17-nucleotide insertion downstream of the primer-binding site appeared to be a common sequence signature specific to most CRF02_AG strains, including 97GH-AG1. 97GH-AG1 showed an R5 phenotype and exerted productive infection in both HOS and NP2 cell infectivity assays, whereas it failed to show a detectable level of progeny production in peripheral blood mononuclear cells (PBMCs). The data may suggest the presence of unknown determinant(s) that dictate efficient replication in PBMCs, but that are not required for replication in immortalized cell lines.
DNA topoisomerase II is the molecular target of bisdioxopiperazine derivatives ICRF-159 and ICRF-193 in Saccharomyces cerevisiae. (1995)

Bisdioxopiperazines such as ICRF-159 and ICRF-193 have been shown to inhibit DNA topoisomerase II. To determine the molecular target of these compounds in vivo, we utilized a yeast genetic system in which the topoisomerase II activity can be modulated. To reduce topoisomerase II activity, we used top2-1 mutant yeast cells that have normal DNA topoisomerase II activity at 25 degrees C but greatly reduced enzyme activity at 30 degrees C, a temperature that is semipermissive for growth. At 25 degrees C top2-1 cells are as sensitive to the ICRF compounds as the wild-type strain; at 30 degrees C the cells became hypersensitive to these agents. In contrast, top2-1 strains become very resistant to the class of topoisomerase II inhibitors such as amsacrine and etoposide, which stabilize the covalent enzyme-DNA intermediate of the enzyme reaction. Overexpression of topoisomerase II from a plasmid-born TOP2 gene results in lower susceptibility to ICRF compounds and higher susceptibility to amsacrine than the parental strain exhibits. These results support the hypothesis that the main cellular target of ICRF compounds is DNA topoisomerase II, and that these compounds, unlike amsacrine and etoposide, inhibit topoisomerase II activity without stabilizing an enzyme-DNA covalent complex.
Properties of a calcitonin receptor and adenylate cyclase in BEN cells, a human cancer cell line. (1980)

A human lung cancer cell line (BEN cells) was found to have a calcitonin-responsive adenylate cyclase. Various calcitonins and synthetic analogs stimulated adenylate cyclase activity withe same relative potency as they show in lowering blood calcium in the rat. Preincubation of the cells with calcitonin, followed by washing, led to loss of subsequent adenylate cyclase response to hormone. This was a dose-dependent phenomenon. The binding of [125I]salmon calcitonin to freshly subcultured cells was studied. The ability of calcitonins and analogs to compete for binding paralleled their efficacies in stimulating adenylate cyclase. Binding was saturable, reversible, and consisted of a single class of noninteracting sites with a mean Kd of 10.75 X 10(-10) M, K of 0.93 X 10(9)/M, and mean receptor number of 2.71 X 10(4)/cell. It is not known whether the calcitonin receptor is inappropriate to the cell of origin of the tumor. The BEN cells provide a means of isolating and studying the properties of the calcitonin receptor and of evaluating the significance for the tumor of a hormone-responsive adenylate cyclase.
A low power, wide linear-range CMOS voltage-controlled oscillator (2002)

A low power CMOS voltage-controlled oscillator (VCO) is presented. Its simple and symmetric structure can provide low-power and low-noise operation, which is comparable to existing architectures in CMOS. The proposed VCO is digitally programmable to have different center frequencies while maintaining its linear voltage-to-frequency characteristic and a desirable VCO gain at tuned frequency. Simulation result shows that the output frequency of 1 GHz is achieved at the power consumption of 3.5 mW.
Physiological stresses inhibit guanine‐nucleotide‐exchange factor in Ehrlich cells (1988)

Previously, we have shown that phosphorylation of the eukaryotic initiation factor eIF-2 alpha increases under several physiological stresses in which protein synthesis is inhibited in Ehrlich ascites tumor cells. As phosphorylated eIF-2 [eIF-2(alpha P)] is a potent inhibitor of guanine nucleotide exchange factor (GEF), it seemed likely that it was responsible for the inhibition. We have assayed GEF activity levels in extracts prepared from Ehrlich cells exposed to three such stresses, namely heat shock, serum deprivation and glutamine deprivation. Activity was estimated by the ability of GEF to enhance the release of [alpha-32P]GDP from purified eIF-2 [a modification of the reticulocyte lysate assay of Matts, R. L. & London, I. M. (1984) J. Biol. Chem. 259, 6708]. GEF activity was reduced from control values in extracts of heat-shocked cells and serum-deprived cells, concomitant with increased eIF-2 alpha phosphorylation. Inhibition of GEF activity in heat-shocked and serum-deprived cells was reversed to control levels by increasing the concentration of purified eIF-2.GDP added as substrate in the GEF assay. Since we have shown elsewhere that eIF-2(alpha P).GDP inhibits GEF by competition with eIF-2.GDP, the complete reversal of inhibition of GEF activity in heat-shocked and serum-deprived cells indicates that inhibition is due solely to phosphorylation of eIF-2 alpha. In glutamine-deprived cells phosphorylation of eIF-2 alpha was increased modestly and GEF activity was reduced but GEF activity could not be fully reversed by addition of eIF-2.GDP, suggesting that GEF may also be regulated in other ways. There are greater amounts of GEF relative to eIF-2 in Ehrlich cells (approximately 50%) compared with rabbit reticulocytes (approximately 20%). This explains the efficient rates of protein synthesis in control Ehrlich cells even though they have 30% of their eIF-2 phosphorylated which is enough to inhibit GEF and initiation in reticulocytes completely but only enough to trap approximately 60% of the GEF in Ehrlich cells.
Determining specific gravity of urine with reagent sticks. (1984)

Journal Article Determining specific gravity of urine with reagent sticks. Get access F C Luft, F C Luft Search for other works by this author on: Oxford Academic Google Scholar G R Aronoff, G R Aronoff Search for other works by this author on: Oxford Academic Google Scholar N A Walker, N A Walker Search for other works by this author on: Oxford Academic Google Scholar R S Sloan, R S Sloan Search for other works by this author on: Oxford Academic Google Scholar N S Fineberg N S Fineberg Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 30, Issue 4, 1 April 1984, Pages 582–583, https://doi.org/10.1093/clinchem/30.4.582 Published: 01 April 1984
The nucleocytoplasmic shuttling of E2F4 is involved in the regulation of human intestinal epithelial cell proliferation and differentiation (2003)

The specific mechanisms controlling the transition from proliferation to terminal differentiation in human intestinal epithelial cells (HIEC) remain largely undefined. Herein, we analyzed the expression and localization of Rb and E2F proteins in well-established normal intestinal epithelial cell models which allow for the re-enactment of the crypt-villus axis in vitro as well as in intact epithelium and in colon cancer cells. We report that (1) expression of E2F1 is down-regulated while E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluency, and terminal differentiation of intestinal cells; (2) concurrently, there is an accumulation of the hypophosphorylated form of the pocket proteins into the nucleus with an increased association of E2F4 with pRb and p130; (3) cells which expressed high levels of nuclear E2F4 are all positive for Ki67 staining in human fetal intestine; (4) activation of HIEC crypt cells by growth factors leads to an increase in the nuclear localization of E2F4 which may be attributable to a decrease in the serine/threonine phosphorylation of this transcription factor; (5) inhibition of p38 MAP kinase with alpha/beta inhibitor SB203580 induces E2F4 translocation into the nucleus and its transcriptional activity. In conclusion, our data suggest a key role for E2F4 in proliferation of human intestinal crypt cells and that its cytoplasmic retention as well as its sequestration by Rb proteins may represent a critical step in initiating cell-cycle exit.
Time Reversal Beamforming for Microwave Breast Cancer Detection (2007)

Microwave radiation is well known as a diagnostic imaging method for many medical applications, for example, early stage breast cancer detection. Microwave detection of breast tumors is a non-ionising, potentially low cost, in vivo modality that relies on the dielectric contrast between healthy and malignant breast tissues. The scattering environment in a breast often appears to be inhomogeneous due to changing dielectric properties of the breast tissues. Time reversal (TR) is an adaptive waveform transmission scheme that utilizes the rich scattering medium to best match to the target response. In this paper, we develop the microwave time reversal beamformer for breast tumor detection. The TR beamforming scheme is examined based upon a breast model using the two-dimensional finite-difference time-domain (FDTD) method. We show that time reversal microwave beamforming is a more robust, higher resolution imaging scheme than conventional beamforming schemes.
Injecting RBAC to secure a Web-based workflow system (2000)

Web-based workflow systems have recently received much attention because they can support dynamic business processes over heterogeneous computing systems. Most existing web-based workflow systems, however, provide minimal security services such as authentication of users and network security. In this paper we describes an experiment in injecting role-based access control (RBAC) into an existing web-based workflow system. Specifically, we ensure that each task can only be executed by users belonging to a specific role. In order to achieve this, we define a simplified RBAC model to meet our needs and describe the security architecture to be applied to an existing web-based workflow system. We describe our implementation using commercial off-the-shelf (COTS) technology to demonstrate the feasibility of this approach. Our implementation uses X.509v3 certificates with role attribute, and employs a user-pull style where the client requests a client certificate from the role-server and presents it to the workflow system. A major goal of our implementation is to have minimal changes to the existing web server and no changes to the browser. We also discuss alternative architecture such as server-pull with LDAP (Lightweight Directory Access Protocol).
Multihop Frontiernet using generalised perfect shuffleinterconnection topology (1995)

A photonic packet switch that uses a multihop frequency-routing interconnection network called Frontiernet is described. A generalised shuffle connectivity is applied to the proposed multihop network where each tunable frequency convertor transmits far fewer consecutive frequency-channels than the number of input/output (I/O) highways. Furthermore, the frequency router can reduce the number of frequency channels used in the network.
Automated verification and test case generation for input validation (2006)

Input validation is essential for any software that deals with input from its external environment. It forms a major part of such software that has intensive interaction with its environment. Through the integration of invariant and empirical properties for implementing input validation, this paper proposes a novel approach for the automation of the following tasks from processing the source code of a program: (1) verification of existence of input validation; (2) generation of test cases to test and demonstrate all the input validations; (3) classification of each validation into the various types defined along with its test case generated. All the empirical properties in the theory have been validated statistically based on open source systems. Our evaluation shows that the proposed approach can help in both testing of input validation features and verifying the adequacy of input control.
In vivo modulation of myelopoiesis by prostaglandin E2. IV. Prostaglandin E2 induction of myelopoietic inhibitory activity. (1988)

Conditioned medium (CM) prepared from bone marrow (BM) or spleen (SPL) cells from mice injected with PGE2 in doses ranging from 0.0001 to 10 micrograms was found to contain an activity inhibitory to the proliferation of granulocyte-monocyte progenitor cells (CFU-GM). This activity was found in medium conditioned for 24 to 48 h, but was not present in medium conditioned for longer time intervals. BM cells from PGE2-treated mice incubated over a concentration range of 0.1 to 1.0 x 10(6) cells/ml and SPL cells over a range of 1.0 to 10 x 10(6) cells/ml produced CM with equivalent degrees of inhibition for CFU-GM proliferation. Titration of this activity revealed a significant inhibitory effect still present at a 1/256 dilution. Inhibitory activity was similar whether or not CM was prepared in the presence or absence of FCS. Inhibition of CFU-GM development was approximately equal in the presence of either PWM SPL CM or L cell CM as sources of CSF activity. Morphologic analysis of CFU-GM revealed an equivalent inhibition of monocyte, monocyte-neutrophil, and neutrophil CFU-GM by the PGE2-stimulated inhibitory activity. Equivalent picogram amounts of PGE were measured in CM derived from BM or SPL cells from either control or PGE2-treated mice, indicating a low probability that injected PGE2 was carried over in the CM and contributed to CFU-GM inhibition. Protease digestion of BM or SPL cell CM from PGE2-treated mice revealed a loss of inhibitory activity after trypsin, chymotrypsin, pronase, and neuraminidase treatment. Inhibitory activity was also ablated by heat treatment at 56 degrees C for 30 min and 100 degrees C for 5 min. Acrylamide-agarose gel filtration of BM CM revealed an active inhibitory fraction in the Mr range of 5.5 to 8.0 kDa. The results of the present study suggest that one of the mechanisms by which PGE2 exerts its in vivo myelopoietic inhibitory action may be by stimulating the production of an inhibitory factor or factors from BM and SPL cells.
Preparation and Characterization of Monodisperse Fe Nanoparticles (2003)

Fe nanoparticles prepared by iron carbonyl decomposition using different methods are compared structurally, chemically, and magnetically. The specific magnetization of the particles was determined from the magnetic moment, the particle size observed by transmission electron microscopy, and the total iron concentration found from calibrated X-ray fluorescence. The volume fraction of oxide is reported for particles of different sizes and for particles made by slightly different techniques.
Integrated ultrasound transducers (1994)

Piezoelectric polymer materials are of interest for high frequency transducer array applications primarily because the array geometry can be achieved using photolithography. The UMIST research team have fabricated up to 64-element polymer transducer arrays. They have adopted a unique approach whereby the pulse control electronics can be mounted in close physical proximity with the transducer array. In order to accomplish this both transmit and receive circuitry has been integrated onto in-house custom designed silicon chip sets. A notable achievement is the design of a monolithic 16-channel programmable pulse generator chip fabricated in 1.5 μm CMOS technology. By employing novel design techniques time delays with 1 ns time resolution and a dynamic range of 2 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">19</sup> have been achieved. A description of the system is provided and preliminary results of beam plotting measurements are given
Glomerular basement membrane-containing immune complexes stimulate tumor necrosis factor and interleukin-1 production by human monocytes. (1989)

The ability of human peripheral blood monocytes to produce tumor necrosis factor (TNF) and interleukin-1 (IL-1) in an in vitro model of immune complex-mediated glomerulonephritis was investigated. When isolated monocytes were incubated with human glomerular basement membrane (GBM) containing anti-GBM immune complexes, both TNF and IL-1 were produced and secreted into the medium. The time course of secretion differed, with IL-1 production being maximal after approximately 8 hours, whereas TNF levels continued to rise for 30 hours. The activities of the monocyte-derived TNF and IL-1 were inhibitable by specific antibodies. No effect was seen when monocytes were incubated separately with either GBM alone or anti-GBM IgG. The levels of TNF and IL-1 released were comparable with those induced by high concentrations of LPS, indicating that production was close to the maximal levels reported for these cells. High levels of TNF and IL-1 also were produced in response to soluble immune complexes. The results show that monocytes can produce significant levels of TNF and IL-1 in response to both surface-bound and soluble immune complexes and provide support for the participation of these monokines in glomerulonephritis.