gebhart/patrepeval huggingface dataset:
A High Throughput Screen for RGS Proteins Using Steady State Monitoring of Free Phosphate Formation (2013)

G-protein coupled receptors are a diverse group that are the target of over 50% of marketed drugs. Activation of these receptors results in the exchange of bound GDP for GTP in the Gα subunit of the heterotrimeric G-protein. The Gα subunit dissociates from the β/γ subunits and both proceed to affect downstream signaling targets. The signal terminates by the hydrolysis of GTP to GDP and is temporally regulated by Regulators of G-protein Signaling (RGS) proteins that act as GTPase Activating Proteins (GAPs). This makes RGS proteins potentially desirable targets for "tuning" the effects of current therapies as well as developing novel pharmacotherapies. Current methods for evaluating RGS activity depend on laborious and/or expensive techniques. In this study we developed a simple and inexpensive assay for the steady state analysis of RGS protein GAP activity, using RGS4, RGS8 and RGS17 as models. Additionally, we report the use of RGS4 as a model for high throughput assay development. After initial setup, this assay can be conducted in a highly parallel fashion with a read time of less than 8 minutes for a 1536-well plate. The assay exhibited a robust Z-factor of 0.6 in a 1536-well plate. We conducted a pilot screen for inhibitors using a small, 2320 compound library. From this screen, 13 compounds were identified as compounds for further analysis. The successful development of this assay for high-throughput screening provides a low cost, high speed, simple method for assessing RGS protein activity.
Infrared sensor by inkjet printing cytochrome c on suspending aluminum electrodes of post CMOS process (2016)

Cytochrome c protein thin film possesses a high temperature coefficient of resistance. In this paper, we systematically investigated the characteristics of cytochrome c, whose absorption coefficient is 65% at wavelengths of 8~12 μm. We found that the changes in resistance resulted from surface roughness. We also discovered that, while cytochrome c improves the temperature coefficient of resistance, a pure protein solution does not conduct well. It needs a buffer solution, acting as an electrolyte, to increase electrical conductance. However, the buffer solution decreases the temperature coefficient. Therefore, optimization of the ratio of cytochrome c protein to buffer solution is required. We determined the best mixing ratio of the protein solution for a sensing material. We then designed a chip for an infrared microbolometer with a MEMS structure of suspended aluminum electrodes. The protein solution was deposited on the sensing pixel using an inkjet printer. The temperature coefficient of resistance, thermal conductance, time constant and responsivity were 25.98%/K, 7.96 × 10-5 W/K, 1.094 ms and 2.57 × 105 V/W at 2 μA bias current, respectively. We experimentally demonstrated integrating cytochrome c protein with a CMOS circuit as a sensing pixel for a longwavelength infrared microbolometer. Based on our experimental results, such a microbolometer array holds promise for the future.
Angiopoietin 2 Is a Partial Agonist/Antagonist of Tie2 Signaling in the Endothelium (2009)

Angiopoietin 2 (Ang2) was originally shown to be a competitive antagonist for Ang1 of the receptor tyrosine kinase Tie2 in endothelial cells (ECs). Since then, reports have conflicted on whether Ang2 is an agonist or antagonist of Tie2. Here we show that Ang2 functions as an agonist when Ang1 is absent but as a dose-dependent antagonist when Ang1 is present. Exogenous Ang2 activates Tie2 and the promigratory, prosurvival PI3K/Akt pathway in ECs but with less potency and lower affinity than exogenous Ang1. ECs produce Ang2 but not Ang1. This endogenous Ang2 maintains Tie2, phosphatidylinositol 3-kinase, and Akt activities, and it promotes EC survival, migration, and tube formation. However, when ECs are stimulated with Ang1 and Ang2, Ang2 dose-dependently inhibits Ang1-induced Tie2 phosphorylation, Akt activation, and EC survival. We conclude that Ang2 is both an agonist and an antagonist of Tie2. Although Ang2 is a weaker agonist than Ang1, endogenous Ang2 maintains a level of Tie2 activation that is critical to a spectrum of EC functions. These findings may reconcile disparate reports of Ang2's effect on Tie2, impact our understanding of endogenous receptor tyrosine kinase signal transduction mechanisms, and affect how Ang2 and Tie2 are targeted under conditions such as sepsis and cancer.
Development of water slurry gasification systems for high-moisture biomass (1986)

The development of a new biomass gasification system by Pacific Northwest Laboratory is described. The system promises to allow the efficient thermochemical conversion of high-moisture biomass feedstocks. The reactor operates at 400 to 450/sup 0/C and 4000 to 5000 psig to allow the gasification of water-biomass slurries containing up to 95% moisture. Initial results of gasification studies are presented. Raw product gases containing up to 95% moisture. Initial results of gasification studies are presented. Raw product gases containing up to 40% methane have been obtained with carbon conversions in excess of 95%. Feedstocks being studied include aquatic plants, agricultural residues, food processing waste and effluent from an anaerobic methane digestor. 11 refs., 4 figs., 4 tabs.
Making distributed applications manageable through instrumentation (2002)

The goal of a management system in a distributed computing environment is to provide a centralized and coordinated view of an otherwise distributed and heterogeneous collection of hardware and software resources. Management systems monitor, analyse and control network resources, system resources, and distributed application programs. Many organizations currently depend on mission-critical distributed applications, a trend that will increase as software engineering tools emerge that make it easier to construct distributed applications. We believe that manageability must be built in to distributed applications from the beginning rather than added in an ad hoc fashion after they have been developed. Just as designing software for usability, testability and maintenance are being addressed in the development process, so must designing for manageability. Application manageability is a research issue of particular interest to us. The work described in this paper focuses on instrumenting processes to allow them to respond to management requests, generate management reports, and maintain information required by the management system. We present an instrumentation architecture to support this, a prototype implementation which includes a class library of standard instrumentation, and a methodology for instrumentation.
Estrogen Activity and Novel Tissue Selectivity ofΔ 8,9-Dehydroestrone Sulfate in Postmenopausal Women (1999)

Recent basic and clinical advances have consolidated the concept of tissue-selective estrogens, i.e. molecules that express different degrees of partial agonist, full agonist or antagonist activity in different tissues or cells. Delta8,9-Dehydroestrone sulfate (delta8,9-DHES) is a conjugated estrogen and a component of conjugated equine estrogens (CEE). It is metabolized in the human in at least a 1:1 ratio to its 17beta form, 17beta-delta8,9-DHES. To evaluate its activity in different clinical and biochemical parameters, a clinical research study was conducted with delta8,9-DHES and estrone sulfate as a comparator in postmenopausal women. Delta8,9-DHES was given orally at a daily dose of 0.125 mg for 12 weeks in a group of 10 women. Two additional groups of women received either estrone sulfate alone (1.25 mg/day) or the combination of delta8,9-DHES and estrone sulfate at the previously specified doses. A significant and consistent suppression of hot flushes (number, severity, and total score) was observed with delta8,9-DHES, reaching more than 95% suppression in all parameters of vasomotor symptoms. This level of activity was equal to that obtained with the much higher dose of estrone sulfate, and it was sustained for the duration of the treatment period (12 weeks). Measurements of a bone resorption marker, i.e. urinary excretion of N-telopeptide, demonstrated that delta8,9-DHES at 8 weeks produced a degree of suppression (40%) similar to that observed with the higher dose of estrone sulfate. Gonadotropin secretion (FSH and LH) was significantly suppressed in women receiving delta8,9-DHES, similar to that observed with estrone sulfate alone or with the combination of the two. Other parameters, such as total cholesterol, low density lipoprotein cholesterol and high density lipoprotein cholesterol were not modified significantly, whereas serum globulins (sex hormone-binding globulin and corticosteroid-binding globulin) showed only marginal increases after delta8,9-DHES administration. Taken together with preclinical data, it is found that delta8,9-DHES is an active estrogen with a distinct pharmacological profile that results in significant clinical activity in vasomotor, neuroendocrine (gonadotropin and PRL) and bone preservation parameters, whereas displaying little or no efficacy, at the dose tested, on other peripheral parameters normally affected by estrogens. Collectively, this information supports the concept that delta8,9-DHES is an integral component of CEE, with distinct tissue selectivity contributing to the CEE's overall clinical activity, and places this estrogen as a distinct member of a novel class of centrally active molecules with unique peripheral tissue selectivity.
Compression, Permeation and Flow Behavior of Wet Nanoparticle Cakes, in situ Tested with a Press-Shear Cell (2002)

A new test apparatus for in situ simulation of the expression operation and rheological testing of compressed cake was developed to combine the compression-permeability cell with a high-performance ring shear tester. The yield loci for water-saturated titania (dS = 200 nm) are measured. These flow parameters can be used to determine in situ the shear strength and compressive strength of a consolidated cake after the pressure filtration steps. The permeability of the compressed cake is characterized by the conventional filter cake resistance versus particle pressure and, for comparison, by an averaged pore size.
Energy absorption mechanism by biological bodies in the near field of dipole antennas above 300 MHz (1992)

The energy absorption mechanism in the close near field of dipole antennas is studied by numerical simulations. All computations are performed and validated applying the three-dimensional multiple multipole software package. The numerical model of the plane phantom is additionally checked by accurate as possible experimental measurements. For the plane phantom, the interaction mechanism can be described well by H-field induced surface currents. The spatial peak specific absorption rate can be approximated within 3 dB by a formula given here based on the incident H-field or antenna current and on the conductivity and permittivity of the tissue. These findings can be generalized to heterogeneous tissues and larger biological bodies of arbitrary shape for frequencies above 300 MHz. The specific absorption rate is mainly proportional to the square of the incident H-field, which implies that in the close near field, the spatial peak specific absorption rate is related to the antenna current and not to the input power.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Electrokinetic Nanoparticle Treatment of Hardened Cement Paste for Reduction of Permeability (2006)

Colloidal nanoparticles were electrokinetically transported into hardened cement paste pores where they underwent chemical reactions resulting in reduced permeability. 20nm silica and 2nm alumina particles were combined with simulated pore fluids to assess precipitate production. One precipitate formed was C-S-H, the binder material native to Portland cement paste. Permeability tests were conducted to study the effect of these processes on hardened cement pastes of high water/cement ratio and of high and low alkali contents. It was observed that 5min treatments using 5V of potential applied over a span of 0.15m is sufficient to drive nanoparticles into the pore system, enabling them to react and produce precipitates. The coefficient of permeability for each paste was reduced by 1–3 orders of magnitude. These findings indicate that reactive nanoparticles can be electrokinetically inserted to reduce the permeability of hardened cement paste, even in the presence of an opposing hydraulic flow.
HeapMon: A helper-thread approach to programmable, automatic, and low-overhead memory bug detection (2006)

The ability to detect and pinpoint memory-related bugs in production runs is important because in-house testing may miss bugs. This paper presents HeapMon, a heap memory bug-detection scheme that has a very low performance overhead, is automatic, and is easy to deploy. HeapMon relies on two new techniques. First, it decouples application execution from bug monitoring, which executes as a helper thread on a separate core in a chip multiprocessor system. Second, it associates a filter bit with each cached word to safely and significantly reduce bug checking frequency—by 95% on average. We test the effectiveness of these techniques using existing and injected memory bugs in SPEC®2000 applications and show that HeapMon effectively detects and identifies most forms of heap memory bugs. Our results also indicate that the HeapMon performance overhead is only 5%, on average—orders of magnitude less than existing tools. Its overhead is also modest: 3.1% of the cache size and a 32-KB victim cache for on-chip filter bits and 6.2% of the allocated heap memory size for state bits, which are maintained by the helper thread as a software data structure.
Consequences of Partial and Total Plantar Fascia Release: A Finite Element Study (2006)

The FE model suggested that plantar fascia release may provide relief of focal stress and therefore could relieve associated heel pain. However, these operative procedures may pose a risk to arch stability and clinically may produce dorsolateral midfoot pain. The initial strategy for treating plantar fasciitis should be nonoperative. If surgery is necessary, partial release of less than 40% of the fascia is recommended to minimize the effect on arch instability and maintain normal foot biomechanics.
A knowledge‐based system to support procurement decision (2005)

Purpose – To propose an infrastructure of a knowledge‐based system to capture and maintain the procurement information and purchasers' knowledge, regarding how to choose partners in the supply chain network, with the adopting of the neural networks that mimic the operation of human brain to generate solution systematically.
Short Sleep Duration Is Associated with Reduced Leptin, Elevated Ghrelin, and Increased Body Mass Index (2004)

Participants with short sleep had reduced leptin and elevated ghrelin. These differences in leptin and ghrelin are likely to increase appetite, possibly explaining the increased BMI observed with short sleep duration. In Western societies, where chronic sleep restriction is common and food is widely available, changes in appetite regulatory hormones with sleep curtailment may contribute to obesity.
People monitoring using face recognition with observation constraints (2005)

We propose a people monitoring system to recognize people by probabilistic inference methods exploiting low-level facial feature and high-level domain knowledge. In particular, the faces of people in the view of a monitoring camera are first detected and modeled. Optimal recognition of people leaving and entering a closed-room is accomplished by exploiting temporal correlation and constraints among the observed face sequence. The optimality is achieved in the sense of maximizing a joint posterior probability of multiple observations. Experimental results of real and synthetic data suggest the efficacy of the proposed system.
Rusia ante la crisis nuclear iraní (2006)

En este ARI se analiza la posicion de Rusia en la crisis nuclear irani para dirimir cuales pueden ser sus posibles mecanismos de resolucion.
Routing Protocols for Delay Tolerant Networks: A Reference Architecture and a Thorough Quantitative Evaluation (2016)

In this paper, we propose a reference architecture for Delay-Tolerant Networking (DTN) routing protocols and a thorough quantitative evaluation of many protocols proposed in the literature. We categorize DTN protocols according to their use of the three techniques that are the key elements of our reference architecture: queue management, forwarding and replication. Queue management orders and manages the messages in the node’s buffer; forwarding selects the messages to be delivered when there is a contact; and finally, replication bounds the number of replicas in the network. Contrary to most previous papers, where either only qualitative comparisons have been presented or only a single category of protocols has been analyzed, in our work, we discuss the results of our experimental activity on many of the DTN protocols in the literature. Our results, which have been obtained both using synthetic and real mobility traces, show that an effective combination of the proposed techniques can significantly improve the performance of the protocols in terms of delivery ratio, overhead and delay.
Quality monitoring for real-time IP media transport over multi-point delivery environment (2008)

As the demand for real-time IP media transport over multi-point delivery environments increases, it is essential to monitor the time-varying status of both network metrics (i.e., delay, jitter, and loss) and system metrics (i.e., CPU and memory utilization). In this paper, we propose and implement a new monitoring system for the high-quality real-time media delivery over multicast-enabled network. It monitors the time-varying conditions of both the multicast network and the node system. When the media performance degrades, the proposed monitoring system attempts to pinpoint the cause of performance degradation to improve the end-to-end performance. The experimental results show that we can effectively infer the cause of performance degradation using the implemented prototype of the proposed monitoring system.
Probabilistic Multiple Cue Integration for Particle Filter Based Tracking (2003)

Abstract. Robust visual tracking has become an important topic in the field of computer vision. The integration of cues such as color, edge strength and motion has proved to be a promising approach to robust visual tracking in situations where no single cue is suitable. In this paper, an algorithm is presented which integrates multiple cues in a probabilistic manner. Specifically the likelihood of each cue is calculated and weighted before Bayes ’ rule is applied to obtain the resultant posterior. This posterior is generally not well represented analytically, and is therefore represented as a set of weighted particles, which is updated at each frame by a particle filter. This paper demonstrates how the combination of multiple cue integration and particle filtering results in a robust tracking method. We also demonstrate how each cue’s weight can be adapted on-line during the tracking procedure. 1
Comment on Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist Size (2008)

ADVERTISEMENT RETURN TO ISSUEPREVCorrespondenceNEXTComment on Safety and Antioxidant Activity of a Pomegranate Ellagitannin-Enriched Polyphenol Dietary Supplement in Overweight Individuals with Increased Waist SizeAna Faria*†§, Rosário Monteiro†#, Isabel Azevedo†, and Conceição Calhau†View Author Information Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; Chemistry Investigation Centre (CIQ), Department of Chemistry, Faculty of Sciences, University of Porto, 4169-007 Porto, Portugal; and Faculty of Nutrition and Food Sciences, University of Porto, 4200-465 Porto, Portugal* Author to whom correspondence should be addressed (e-mail [email protected]).†Department of Biochemistry.§Chemistry Investigation Centre.#Faculty of Nutrition and Food Sciences.Cite this: J. Agric. Food Chem. 2008, 56, 24, 12143–12144Publication Date (Web):November 3, 2008Publication History Received18 February 2008Published online3 November 2008Published inissue 24 December 2008https://pubs.acs.org/doi/10.1021/jf802383qhttps://doi.org/10.1021/jf802383qarticle-commentaryACS PublicationsCopyright © 2008 American Chemical Society. This publication is available under these Terms of Use. Request reuse permissions This publication is free to access through this site. Learn MoreArticle Views1029Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail PDF (57 KB) Get e-AlertscloseSUBJECTS:Beverages,Food,Peptides and proteins,Phytochemistry,Rodent models Get e-Alerts
Subchip multipath delay estimation for downlink WCDMA system based on Teager-Kaiser operator (2003)

Accurate detection and estimation of overlapping fading multipath components is vital for many communication systems, particularly for positioning technologies. Traditional approaches used for channel estimation generally fail in estimating closely-spaced multipath components in code-division multiple access (CDMA) systems. Here, we present a highly efficient technique for asynchronous downlink WCDMA multipath delay estimation with subchip resolution capability based on nonlinear Teager-Kaiser operator concept. The behavior of this technique is influenced considerably by the pulse shape waveform. Both rectangular and root raised cosine pulse shaping filters are considered.
New Strategies for the Development of an Asymmetric Version of the Baylis–Hillman Reaction (2000)

The development of an efficient, asymmetric variant of the Baylis–Hillman reaction for the synthesis of enantiomerically pure α-methylene-β-hydroxycarbonyl compounds (see scheme) is still a very rewarding goal. Interesting recent approaches for the solution of this problem include the use of chiral auxiliaries and bases and the thorough optimization of reaction conditions.