gebhart/patrepeval huggingface dataset:
Ketone Synthesis by the Grignard Reaction with Acid Chlorides in Presence of Ferric Chloride (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTKetone Synthesis by the Grignard Reaction with Acid Chlorides in Presence of Ferric ChlorideJAMES CASON and KENNETH W. KRAUSCite this: J. Org. Chem. 1961, 26, 6, 1768–1772Publication Date (Print):June 1, 1961Publication History Published online1 May 2002Published inissue 1 June 1961https://pubs.acs.org/doi/10.1021/jo01065a018https://doi.org/10.1021/jo01065a018research-articleACS PublicationsRequest reuse permissionsArticle Views646Altmetric-Citations13LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Traumatic Brain Injury in Adult Rats Causes Progressive Nigrostriatal Dopaminergic Cell Loss and Enhanced Vulnerability to the Pesticide Paraquat (2011)

Parkinson's disease (PD) is a neurodegenerative disorder characterized by the loss of nigrostriatal dopaminergic neurons and the accumulation of alpha-synuclein. Both traumatic brain injury (TBI) and pesticides are risk factors for PD, but whether TBI causes nigrostriatal dopaminergic cell loss in experimental models and whether it acts synergistically with pesticides is unknown. We have examined the acute and long-term effects of TBI and exposure to low doses of the pesticide paraquat, separately and in combination, on nigrostriatal dopaminergic neurons in adult male rats. In an acute study, rats received moderate TBI by lateral fluid percussion (LFP) injury, were injected with saline or paraquat (10 mg/kg IP) 3 and 6 days after LFP, were sacrificed 5 days later, and their brains processed for immunohistochemistry. TBI alone increased microglial activation in the substantia nigra, and caused a 15% loss of dopaminergic neurons ipsilaterally. Paraquat increased the TBI effect, causing a 30% bilateral loss of dopaminergic neurons, reduced striatal tyrosine hydroxylase (TH) immunoreactivity more than TBI alone, and induced alpha-synuclein accumulation in the substantia nigra pars compacta. In a long-term study, rats received moderate LFP, were injected with saline or paraquat at 21 and 22 weeks post-injury, and were sacrificed 4 weeks later. At 26 weeks post injury, TBI alone induced a 30% bilateral loss of dopaminergic neurons that was not exacerbated by paraquat. These data suggest that TBI is sufficient to induce a progressive degeneration of nigrostriatal dopaminergic neurons. Furthermore, TBI and pesticide exposure, when occurring within a defined time frame, could combine to increase the PD risk.
Effect of Placing a Diffuser around a Wind Turbine (2000)

Abstract An actuator disc CFD model of the flow through a wind turbine in a diffuser is developed and validated. Further, it is shown theoretically from a 1D analysis that the Betz limit can be exceeded by a factor proportional to the relative increase in mass flow through the rotor induced by the diffuser. The theoretical 1D result is verified by the CFD model. Copyright © 2000 John Wiley & Sons, Ltd.
Deposition of idiotype-anti-idiotype immune complexes in renal glomeruli after polyclonal B cell activation. (1982)

We investigated the possible role of idiotypic interactions in the pathogenesis of the glomerular lesions observed in mice undergoing polyclonal B cell activation. BALB/c mice were studied for the presence of renal deposits of T15 idiotype-anti-T15 idiotype-immune complexes (IC) after injection of bacterial lipopolysaccharides (LPS). The T15 idiotype is the major idiotype of BALB/c mice anti-phosphorylcholine (PC) antibodies, which are cross-reactive with the idiotype of the TEPC-15 myeloma protein. This model was used because T15 idiotype-anti-T15 idiotype IC have been detected in the circulation of BALB/c mice after polyclonal B cell activation. First, an idiotype-specific immunofluorescence technique allowed us to detect T15 idiotype-bearing immunoglobulins in glomeruli from day 6 to day 28 after LPS injection. Second, fluorescein isothiocyanate-conjugated TEPC-15 myeloma protein was found to localize in the glomeruli after in vivo injection 18 d after LPS administration. This renal localization was shown to be idiotype-specific and could be quantified in a trace-labeling experiment. Third, kidney-deposited immunoglobulins of mice injected with LPS were eluted, radiolabeled, and analyzed by radioimmunoassay. Both T15 idiotype-bearing immunoglobulins and anti-T15 idiotype antibodies were detected in the eluates, providing further evidence for a renal deposition of T15 idiotype-anti-T15 idiotype IC. Polyclonal B cell activation is likely to result in a simultaneous triggering of many idiotypic clones and of corresponding anti-idiotypic clones represented in the B cell repertoire. This could lead to the formation of a variety of idiotype-anti-idiotype IC that could participate in the development of glomerular lesions.
Isolation of an immunosuppressive peptide fraction from the serum of cancer patients. (1975)

An immunosuppressive peptide fraction was isolated by means of gel filtration, membrane partition, and ion-exchange chromatography from the sera of patients hospitalized for cancer. The resulting peptide fraction, which was heterogeneous as judged by high-voltage electrophoresis, was found to suppress both phytohemagglutinin-induced proliferation of lymphocytes in vitro and the in vivo induction of splenic plaque-forming cells in mice. The specific activity of the peptide fraction, which was isolated from the sera of cancer patients, was significantly increased over that of the unfractionated starting material. Moreover, in control experiments, when the sera of normals or non-cancer-bearing hospitalized individuals were subjected to the same chromatographic techniques, no active peptide fraction could be obtained.
Meningeal cells induce dopaminergic neurons from embryonic stem cells (2008)

Neural induction of midbrain dopaminergic (DA) neurons from embryonic stem (ES) cells can be achieved by culturing them on a bone marrow-derived stromal cell line, PA6, which possesses stromal cell-derived inducing activity (SDIA). The mechanism of SDIA is unknown, but clinical application of ES cell transplantation requires the use of defined factors for DA neuron induction. Here, we demonstrate that meningeal cells harvested from the developing dura can induce DA neuron differentiation from mouse and human ES cells, as assessed by midbrain DA marker expression and secretion of DA in response to potassium stimuli. Intriguingly, the inductive strength of meningeal cells depends on their developmental stage, with those harvested from embryonic day 18 embryos showing the highest activity. Among six soluble factors known to be involved in DA neuron differentiation, only Wnt-5a and transforming growth factor-beta3 were expressed by both meningeal and PA6 cells, and the expression of Wnt-5a correlated with the DA neuron induction activity of these cells. Furthermore, the induction of DA neuron differentiation by PA6 cell-conditioned medium was reversed by addition of a Wnt-5a neutralizing antibody, whereas recombinant Wnt-5a promoted DA neuron induction when cells were cultured on Matrigel. These results indicate that meningeal cells can be used as feeders to induce DA neurons from ES cells, and that Wnt-5a plays an important role in DA neuron induction by SDIA.
A large size MEMS scanning mirror for speckle reduction application (2017)

Based on the micro electronic mechanical system (MEMS) processing, a large size 2-D scanning mirror driven by electromagnetic force was designed and implemented in this paper. Here we fabricated micromirror with the silicon wafer and electroplated soft Ni film on the backside of mirror. The no-coil structural micro-mirror prevented heat produced on the mirror effectively. The scanning resonant frequency for the mirror is 674Hz along slow axis, and 1870Hz along fast axis. Finally, the MEMS mirror was used in laser display system to reduce the laser speckle. With the 2-D scanning of the MEMS mirror, the speckle contrast could be reduced to 4.2%. We demonstrated that the image quality of a laser display system could be greatly improved with the MEMS mirror.
Gene Expression Profile Identifies Tyrosine Kinase c-Met as a Targetable Mediator of Antiangiogenic Therapy Resistance (2013)

These findings support the role of c-Met in survival in hypoxia and invasion, features associated with antiangiogenic therapy resistance, and growth and therapeutic resistance of xenografts resistant to antiangiogenic therapy. Therapeutically targeting c-Met could prevent or overcome antiangiogenic therapy resistance.
The role of a liquid crystal oligomer in stabilizing blue phases (2007)

A novel liquid crystal oligomer, 4‐(4‐cyanophenyl)phenyl 4‐octyloxy‐2‐{7‐{4‐[4‐(4‐hexylphenyl)‐2,3‐difluorophenyl]phenyloxy}heptanoyloxy}benzoate, was prepared and found to exhibit a wide temperature range of a nematic phase in spite of being far from a rod‐like structure. Furthermore, the compound induced blue phases in the mixture with a chiral smectic liquid crystal. The molecular shape and its 2,3‐difluorophenyl unit play a role in stabilizing the blue phases.
Human Thyroid-stimulating Hormone (hTSH) Subunit Gene Fusion Produces hTSH with Increased Stability and Serum Half-life and Compensates for Mutagenesis-induced Defects in Subunit Association (1997)

The human thyroid-stimulating hormone (hTSH) subunits alpha and beta are transcribed from different genes and associate noncovalently to form the bioactive hTSH heterodimer. Dimerization is rate-limiting for hTSH secretion, and dissociation leads to hormone inactivation. Previous studies on human chorionic gonadotropin (hCG) and human follicle-stimulating hormone had shown that it was possible by subunit gene fusion to produce a bioactive, single chain hormone. However, neither the stability nor the clearance from the circulation of such fused glycoprotein hormones has been studied. We show here that genetic fusion of the hTSH alpha- and beta-subunits using the carboxyl-terminal peptide of the hCG beta-subunit as a linker created unimolecular hTSH whose receptor binding and bioactivity were comparable to native hTSH. Interestingly, the fused hTSH had higher thermostability and a longer plasma half-life than either native or dimeric hTSH containing the hCG beta-subunit-carboxyl-terminal peptide, suggesting that dimer dissociation may contribute to glycoprotein hormone inactivation in vivo. In addition, we show for the first time that synthesis of hTSH as a single polypeptide chain could overcome certain mutagenesis-induced defects in hTSH secretion, therefore enabling functional studies of such mutants. Thus, in addition to prolongation of plasma half-life, genetic fusion of hTSH subunits should be particularly relevant for the engineering of novel analogs where desirable features are offset by decreased dimer formation or stability. Such methods provide a general approach to expand the spectrum of novel recombinant glycoprotein hormones available for in vitro and in vivo study.
The effect of sorbed penetrants on the aging of previously dilated glassy polymer powders. I. Lower alcohol and water sorption in poly(methyl methacrylate) (1987)

Abstract Sorption kinetics and equilibria for methanol, ethanol, and n ‐propanol in 0.544 μm diameter poly(methyl methacrylate) microspheres were determined at 35°C over a wide range of relative pressures. Sorption isotherms were concave to the pressure axis at low relative pressures and convex to the pressure axis at higher relative pressures. These results, considered in the context of recently reported data for high pressure sorption of gases in polymeric glasses, suggest that the S‐shaped isotherms reported here are examples of a generalized isotherm which describes sorption behavior of all penetrants in glassy polymers if an appropriate range of concentration is traversed by the experimental protocol. The effects of dialating the microspheres by preswelling with methanol were studied by subsequent low pressure sorption of water, methanol, ethanol, and n ‐propanol at 35°C. The preswollen microspheres exhibited initially higher sorption capacities than the as‐Received samples, but tended to consolidate with time following the preswelling treatment. The aging process, monitored by periodic short‐term sorption with the various penetrant probes, was arrested by contacting the microspheres with an activity of n ‐propanol sufficient to maintain a sorbed concentration of approximately 1 wt %. The aging was significantly retarded by the presence of low concentrations of water and ethanol. Conversely, the aging process appeared to be essentially unaffected by the presence of correspondingly low concentrations of methanol. The complex kinetics describing the sorption of the various penetrants ranged from Fickian diffusion to polymer relaxation‐controlled absorption, depending upon penetrant, relative pressure, and prior exposure history. The low temperature preswelling of the microspheres markedly increased the rate of sorption as well as the respective apparent equilibrium sorption.
Image quality analysis for dual energy subtraction imaging with a femtosecond laser-based hard X-ray source (2001)

We present a study of image quality for dual energy subtraction imaging using an iodinated contrast agent and a femtosecond laser-based hard X-ray source. The INRS CPA laser (400 fs pulse focused on solid targets in a 3 /spl mu/m spot at 4 /spl times/ 10/sup 18/ W cm/sup -2/) was used to create a bright hard X-ray source (conversion efficiency of 10/sup -5/ in the characteristic K/sub /spl prop// line emission, 12 /spl mu/m X-ray source diameter). A model of image quality has been developed and been benchmarked with specific experiments using specially made angiography phantoms.
M2 Growth in 1995: A Return to Normalcy? (1995)

A discussion of M2's demise as a reliable indicator of financial conditions in the economy, and a look at recent evidence suggesting that even though the aggregate has been behaving more normally over the past year or so, it is unlikely to regain its status as a key policy guide any time soon.
Six-Step MMC-Based High Power DC-DC Converter (2018)

Both DC collection and transmission grids have enjoyed a lot of popularity lately. In order to successfully take advantage of the benehts offered by DC systems, reliable connection between two networks of different voltage levels has to be obtained. This paper presents high step-down ratio, isolated DC-DC converter intended to connect high/medium voltage grid with a low voltage DC grid. Proposed converter combines two Modular Multilevel Converters in series at high/medium voltage side with conventional Six-Step Converter at low voltage side, interfaced by means of Three Winding Three-Phase transformer. Basic operating principles, along with design and sizing rules, are presented and supported by simulation results.
Cytotoxicity and metabolism of alkyl phospholipid analogues in neoplastic cells. (1986)

The cytotoxic response of several types of neoplastic cells to analogues of unnatural alkyl phospholipids (e.g., rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine) has been partially attributed to their accumulation as a result of the low activity of the alkyl cleavage enzyme (a tetrahydropteridine-dependent monooxygenase) in tumor cells. We tested this possibility by comparing the alkyl cleavage enzyme activity in cells that exhibit differences in sensitivity toward the cytotoxic effects of the rac-1-hexadecyl-2-methoxy-glycero-3-phosphocholine. Human promyelocytic leukemia cells (HL-60), a cell line highly sensitive to the cytotoxic alkyl phospholipid analogue, possessed an alkyl cleavage enzyme activity (0.25 pmol/min/microgram protein) similar to that found in three cell types known to be relatively resistant to the cytotoxic activity of the analogue: immature human promyeloblastic leukemia cells (K562) (0.22 pmol/min/microgram protein), human polymorphonuclear neutrophils (0.34 pmol/min/microgram protein), and Madin-Darby canine kidney cells (0.37 pmol/min/microgram protein). Moreover, our results indicate that the cytotoxic rac-1-octadecyl-2-methoxy-glycero-3-phosphocholine analogue is not a substrate for the alkyl cleavage enzyme with an active microsomal preparation of the enzyme from rat liver; cleavage of this analogue was 200-fold less than the rate obtained with 1-octadecylglycerol as substrate. In cultures of either sensitive or resistant type cells, approximately 90% of the added rac-1-[9',10'-3H]octadecyl-2-methoxy-glycero-3-phosphocholine was not metabolized during a 24-h incubation. The amount of radiolabel in fatty acids, a major product of alkyl cleavage activity, was small, and essentially identical amounts were produced in all four cell types [3.1 +/- 0.2% (SD)]. These data indicate that differences in the cellular activities of the alkyl cleavage enzyme are not responsible for the differential cytotoxic responses between normal and specific types of neoplastic cells toward rac-1-octadecyl-2-methoxy-glycero-3-phosphocholine. On the other hand, the cellular uptake of the alkyl phospholipids could be a factor in explaining the cytotoxic response of certain tumor cells, since more radiolabeled 1-octadecyl-2-methoxy-glycero-3-phosphocholine was associated with the susceptible HL-60 cells than with the resistant cell types. Autoradiography revealed that the radiolabeled 2-methoxy analogue accumulates at the periphery of HL-60 leukemia cells, whereas the label was more uniformly distributed in polymorphonuclear neutrophils and K562 cells.(ABSTRACT TRUNCATED AT 400 WORDS)
Effect of diverse categories of drugs on human colon tumour cell proliferation. (1991)

The effect of a number of drugs belonging to different therapeutic categories on cell proliferation in a human colon cancer cell line (HT 29) was studied. Generally, drugs classified as calcium antagonists had a moderate to strong inhibitory effect on cell growth. The EC50 value for prenylamine, perhexiline and bepridil was 10 microM. The so-called calcium channel blockers (verapamil, nifedipine, diltiazem) were less effective. With the exception of the sodium selective ionophore, monensin, which was extremely potent (EC50 less than 0.1 microM), calmodulin antagonism appeared to be a common feature of compounds inhibiting cell proliferation of human colon tumour cells.
Early commitment of naïve human CD4<sup>+</sup> T cells to the T follicular helper (T<sub>FH</sub>) cell lineage is induced by IL‐12 (2009)

T follicular helper (T(FH)) cells are a specialized subset of CD4(+) T cells that localize to B-cell follicles, where they are positioned to provide help for the induction of optimal humoral immune responses. Key features of T(FH) cells are the expressions of CXCR5, ICOS, interleukin (IL)-21 and BCL-6. The requirements for human T(FH) cell development are unknown. Here we show that IL-6, IL-12, IL-21 and IL-23 are capable of inducing IL-21 expression in naïve CD4(+) T cells isolated from human tonsils, peripheral blood and cord blood. However, only IL-12 induced sustained expressions of CXCR5 and ICOS on these activated naïve CD4(+) T cells, and endowed them with the ability to provide increased help to B cells for their differentiation into immunoglobulin-secreting cells. The effects of IL-12 were independent of interferon-gamma and T-bet, and associated with upregulation of BCL-6 expression. Thus, these cytokines, particularly IL-12, are likely to act at an early stage during dendritic cell-mediated priming of naïve CD4(+) T cells into a T(FH) cell fate, and thus underpin antibody-mediated immunity.
Chemically modified hCFTR mRNAs recuperate lung function in a mouse model of cystic fibrosis (2018)

Gene therapy has always been a promising therapeutic approach for Cystic Fibrosis (CF). However, numerous trials using DNA or viral vectors encoding the correct protein resulted in a general low efficacy. In the last years, chemically modified messenger RNA (cmRNA) has been proven to be a highly potent, pulmonary drug. Consequently, we first explored the expression, function and immunogenicity of human (h)CFTR encoded by cmRNA<sup>hCFTR</sup> in vitro and ex vivo, quantified the expression by flow cytometry, determined its function using a YFP based assay and checked the immune response in human whole blood. Similarly, we examined the function of cmRNA<sup>hCFTR</sup> in vivo after intratracheal (i.t.) or intravenous (i.v.) injection of the assembled cmRNA<sup>hCFTR</sup> together with Chitosan-coated PLGA (poly-D, L-lactide-co-glycolide 75:25 (Resomer RG 752 H)) nanoparticles (NPs) by FlexiVent. The amount of expression of human hCFTR encoded by cmRNA<sup>hCFTR</sup> was quantified by hCFTR ELISA, and cmRNA<sup>hCFTR</sup> values were assessed by RT-qPCR. Thereby, we observed a significant improvement of lung function, especially in regards to FEV<sub>0.1</sub>, suggesting NP-cmRNA<sup>hCFTR</sup> as promising therapeutic option for CF patients independent of their CFTR genotype.
Immunocytochemical localization of albumin in the secretory apparatus of rat liver parenchymal cells. (1981)

The localization of albumin was investigated in rat liver, fixed by perfusion, with peroxidase-labeled monospecific antibodies against rat serum albumin purified by affinity chromatography. By light microscopy, albumin is present uniformly in all parenchymal cells with no difference in the intensity of reaction in the different parts of hepatic lobules. By electron microscopy, albumin is localized in the entire secretory apparatus including the rough and smooth endoplasmic reticulum, Golgi complex, and secretory vacuoles. In the rough endoplasmic reticulum, focal negative segments are interposed between positive regions. In the Golgi region, albumin is found both in stacked cisternae and at the trans aspect in the portion called GERL (Golgi-endoplasmic reticulum--lysosome). Whereas albumin and lipoprotein particles are separated in terminal dilatations of the endoplasmic reticulum and in the cisternae on the cis face of the Golgi apparatus, they are usually intermixed in vacuoles of the trans face. Similarly, most secretion vacuoles below the sinusoidal lining contain albumin and lipoprotein particles together, although a few are also found with only one secretory product. These observations suggest that, after synthesis in the rough endoplasmic reticulum, albumin is segregated into smooth transitional elements and transported to the Golgi region where it is packaged together with other secretory products such as lipoproteins. These secretion vacuoles move up the sinusoidal surface, where they are discharged. The possible involvement of GERL in the proteolytic cleavage of proalbumin to albumin is considered.