gebhart/patrepeval huggingface dataset:
Validation and Application of an Assay for Deoxyribonucleic Acid to Estimate Concentrations of Bull Sperm (1990)

Spectrophotometers are used for estimating sperm concentrations from raw ejaculates in semen processing laboratories. Unfortunately, these instruments have a limited detection spectrum and do not permit accurate quantification of sperm numbers in highly diluted or concentrated samples. The objectives of this study were to validate a DNA assay for quantification of sperm numbers in extended or undiluted semen samples and to determine precision of the assay. The principle of the assay is based upon a fluorescent dye that binds to adenine-thymine base pairs in double-stranded DNA. Semen samples and calf thymus DNA standards were sonicated in 2 M NaCl buffer with 1 mM EDTA. The DNA content of samples was compared to standards of calf thymus DNA using fluorometry. Sensitivity of the assay was determined to be 1.4 x 10(5) sperm cells. Concentrations of sperm estimated from DNA assay values did not differ from flow cytometric cell counts. Assays were performed in three different laboratories, using different equipment, to assess the assay's repeatability. Estimates of sperm concentrations determined by the DNA assay were similar, regardless of location and source of equipment used to perform the assays. This assay fulfills statistical criteria for being sensitive, accurate, and repeatable, and it can be employed in laboratories processing semen for artificial insemination as a tool for spectrophotometer calibration, a check for straw filling accuracy, or to quantify sperm numbers in extended, packaged semen.
What's new in the literature: An update of new research since the original <scp>WHS</scp> diabetic foot ulcer guidelines in 2006 (2014)

The objective of the paper was to update the diabetic foot ulcer guidelines that were previously published in 2006. We performed a key word search using MEDLINE and Cochrane reviews for publication between January 2006 and January 2012. Articles that fit the inclusion criteria were reviewed and the previous guidelines were updated.
Bifunctional superparamagnetic surface molecularly imprinted polymer core-shell nanoparticles (2009)

Superparamagnetic surface molecularly imprinted polymer core-shell nanoparticles were prepared via surface atom transfer radical polymerization (ATRP) using the ATRP agent functionalized Fe3O4 support as the chain transfer agent.
Inactivation of Two <i>Dictyostelium discoideum</i> Genes, <i>DdPIK1</i> and <i>DdPIK2,</i> Encoding Proteins Related to Mammalian Phosphatidylinositide 3-kinases, Results in Defects in Endocytosis, Lysosome to Postlysosome Transport, and Actin Cytoskeleton Organization (1997)

Phosphatidylinositide 3-kinases (PI3-kinases) have been implicated in controlling cell proliferation, actin cytoskeleton organization, and the regulation of vesicle trafficking between intracellular organelles. There are at least three genes in Dictyostelium discoideum. DdPIK1, DdPIK2, and DdPIK3, encoding proteins most closely related to the mammalian 110-kD PI-3 kinase in amino acid sequence within the kinase domain. A mutant disrupted in DdPIK1 and DdPIK2 (delta ddpik1/ddpik2) grows slowly in liquid medium. Using FITC-dextran (FD) as a fluid phase marker, we determined that the mutant strain was impaired in pinocytosis but normal in phagocytosis of beads or bacteria. Microscopic and biochemical approaches indicated that the transport rate of fluid-phase from acidic lysosomes to non-acidic postlysosomal vacuoles was reduced in mutant cells resulting in a reduction in efflux of fluid phase. Mutant cells were also almost completely devoid of large postlysosomal vacuoles as determined by transmission EM. However, delta ddpik1/ddpik2 cells functioned normally in the regulation of other membrane traffic. For instance, radiolabel pulse-chase experiments indicated that the transport rates along the secretory pathway and the sorting efficiency of the lysosomal enzyme alpha-mannosidase were normal in the mutant strain. Furthermore, the contractile vacuole network of membranes (probably connected to the endosomal pathway by membrane traffic) was functionally and morphologically normal in mutant cells. Light microscopy revealed that delta ddpik1/ddpik2 cells appeared smaller and more irregularly shaped than wild-type cells; 1-3% of the mutant cells were also connected by a thin cytoplasmic bridge. Scanning EM indicated that the mutant cells contained numerous filopodia projecting laterally and vertically from the cell surface, and fluorescent microscopy indicated that these filopodia were enriched in F-actin which accumulated in a cortical pattern in control cells. Finally, delta ddpik1/ddpik2 cells responded and moved more rapidly towards cAMP. Together, these results suggest that Dictyostelium DdPIK1 and DdPIK2 gene products regulate multiple steps in the endosomal pathway, and function in the regulation of cell shape and movement perhaps through changes in actin organization.
ELPIDA: a general architecture for medical imaging systems supporting telemedicine applications (1995)

During the next years, profound changes are expected in computer and communication technologies that will offer the medical imaging systems (MIS) industry a challenge to develop advanced telemedicine applications of high performance. Medical industry,
vendors, and specialists need to agree on a universal MIS structure that will provide a stack of functions, protocols, and interfaces suitable for coordination and management of high-level image consults, reports, and review activities. Doctors and engineers have worked together to determine the types, targets, and range of such activities within a medical group working domain and to posit their impact on MIS structure. As a result, the fundamental MIS functions have been posed and organized in the form of a general MIS architecture, denoted as ELPIDA. The structure of this architecture was kept as simple as possible to allow its extension to diverse multimode operational schemes handling medical and conversational audiovisual information of different classes. The fundamentals of ELPIDA and pulmonary image diagnostic aspects have been employed for the
development of a prototype MIS.
In Vivo Evaluation of Coralline Hydroxyapatite and Direct Current Electrical Stimulation in Lumbar Spinal Fusion (1999)

Direct-current electrical stimulation increased fusion rates in a dose-dependent manner in a rabbit spinal fusion model. Coralline hydroxyapatite is an osteoconductive bone graft substitute, and thus requires an osteoinductive stimulus to ensure reliable fusion rates. Furthermore, coralline hydroxyapatite and direct current electrical stimulation can be used together to increase fusion rates in a rabbit spinal fusion model while avoiding the morbidity associated with harvesting iliac crest bone.
Self-electrochemiluminescence of CdTe nanocrystals capped with 2-diethylaminoethanethiol (2017)

We successfully synthesized 2-diethylaminoethanethiol (DEAET)-protected CdTe nanocrystals (CdTe-DEAET NCs) by exchanging DEAET onto 3-mercaptopropionic acid (MPA)-capped CdTe NCs (CdTe-MPA NCs) for the first time. Noticeable blue-shifts on the UV-vis absorption and photoluminescence spectra, the decrease of particle sizes and strong anodic ECL emission from CdTe-DEAET NCs were observed.
Switchable Adhesion: On‐Demand Bonding and Debonding (2022)

Adhesives have a long and illustrious history throughout human history. The development of synthetic polymers has highly improved adhesions in terms of their strength and environmental tolerance. As soft robotics, flexible electronics, and intelligent gadgets become more prevalent, adhesives with changeable adhesion capabilities will become more necessary. These adhesives should be programmable and switchable, with the ability to respond to light, electromagnetic fields, thermal, and other stimuli. These requirements necessitate novel concepts in adhesion engineering and material science. Considerable studies have been carried out to develop a wide range of adhesives. This review focuses on stimuli-responsive material-based adhesives, outlining current research on switchable and controlled adhesives, including design and manufacturing techniques. Finally, the potential for smart adhesives in applications, and the development of future adhesive forms are critically suggested.
Optimization of nucleated red blood cell (NRBC) recovery from maternal blood collected using both layers of a double density gradient (2001)

The isolation of fetal nucleated red blood cells (NRBC) from maternal blood represents a promising approach to non-invasive prenatal diagnosis. However, the number of fetal NRBC in maternal circulation is quite low and therefore difficult to isolate. An enrichment procedure in which both layers from a double density 1.077/1.107 g/ml gradient are collected was optimized, followed by MACS selection using non-commercial monoclonal antibodies. The influence of the delay in processing maternal blood on the NRBC distribution in both interfaces of the gradient was also studied in cord blood and peripheral maternal blood samples. A significant increase in the number of NRBC isolated from maternal blood was achieved by collecting both layers of the double density gradient compared with the previous protocol in which only the lower layer was recovered. Cord blood samples showed significant differences in the number of NRBC recovered when processed at 24 instead of within 3 h. This effect was also observed in the number of NRBC collected only from the upper layer of peripheral maternal blood samples. Therefore, in order to minimize the target cell losses, it is advisable to process the maternal blood samples as soon as possible.
Development of a Mouse Monoclonal Antibody Cocktail for Post-exposure Rabies Prophylaxis in Humans (2009)

As the demand for rabies post-exposure prophylaxis (PEP) treatments has increased exponentially in recent years, the limited supply of human and equine rabies immunoglobulin (HRIG and ERIG) has failed to provide the required passive immune component in PEP in countries where canine rabies is endemic. Replacement of HRIG and ERIG with a potentially cheaper and efficacious alternative biological for treatment of rabies in humans, therefore, remains a high priority. In this study, we set out to assess a mouse monoclonal antibody (MoMAb) cocktail with the ultimate goal to develop a product at the lowest possible cost that can be used in developing countries as a replacement for RIG in PEP. Five MoMAbs, E559.9.14, 1112-1, 62-71-3, M727-5-1, and M777-16-3, were selected from available panels based on stringent criteria, such as biological activity, neutralizing potency, binding specificity, spectrum of neutralization of lyssaviruses, and history of each hybridoma. Four of these MoMAbs recognize epitopes in antigenic site II and one recognizes an epitope in antigenic site III on the rabies virus (RABV) glycoprotein, as determined by nucleotide sequence analysis of the glycoprotein gene of unique MoMAb neutralization-escape mutants. The MoMAbs were produced under Good Laboratory Practice (GLP) conditions. Unique combinations (cocktails) were prepared, using different concentrations of the MoMAbs that were capable of targeting non-overlapping epitopes of antigenic sites II and III. Blind in vitro efficacy studies showed the MoMab cocktails neutralized a broad spectrum of lyssaviruses except for lyssaviruses belonging to phylogroups II and III. In vivo, MoMAb cocktails resulted in protection as a component of PEP that was comparable to HRIG. In conclusion, all three novel combinations of MoMAbs were shown to have equal efficacy to HRIG and therefore could be considered a potentially less expensive alternative biological agent for use in PEP and prevention of rabies in humans.
Multiresponsive Rolling Circle Amplification for DNA Logic Gates Mediated by Endonuclease (2014)

Rolling circle amplification (RCA), an efficient isothermal amplification method allowing the polymerase-mediated generation of long single-stranded DNA molecules made of tandem repeats, has been widely used in biomedical and nanotechnology fields due to structural and compositional versatility of its components. In this work, we confer multiresponsiveness to RCA reactions by designing dumbbell-shaped DNA templates and hairpin probes containing different endonuclease cleavage sites. Endonucleases trigger the release of RCA primers or the cleavage of DNA templates, which controls subsequent RCA reactions. A set of one-input and two-input DNA logic gates, which use endonucleases or hairpin probes as inputs, including YES, NOT, AND, OR, NOR, and INHIBIT, are constructed on the basis of our proposed multiresponsive RCA reactions. We demonstrate flexibility and scalability of these logic gates by integrating them to fabricate more complex three-input logic circuits (AND-OR and NOR-AND circuits). Moreover, our strategy is used to construct an assay system for endonuclease activity. Our proposed method might be applicable in the multichannel detection of endonucleases, nucleic acids, and other biomolecules.
Multi-touch gesture interactions and deformable geometry for 3D edition on touch screen (2015)

Interactions gestuelles multi-point et géométrie déformable pour l'édition 3D sur écran tactile: Malgré les progrès en capture d'objets réels et en génération procédurale, la création de contenus pour les mondes virtuels ne peut se faire sans interaction humaine. Cette thèse propose d'exploiter les nouvelles technologies tactiles (écrans "multi-touch") pour offrir une interaction 2D simple et intuitive afin de naviguer dans un environnement virtuel, et d'y manipuler, positionner et déformer des objets 3D. En premier lieu, nous étudions les possibilité et les limitations gestuelles de la main et des doigts lors d'une interaction sur écran tactile afin de découvrir quels gestes semblent les plus adaptés à l'édition des environnements et des objets 3D. En particulier, nous évaluons le nombre de degré de liberté efficaces d'une main humaine lorsque son geste est contraint à une surface plane. Nous proposons également une nouvelle méthode d'analyse gestuelle par phases permettant d'identifier en temps réel les mouvements clés de la main et des doigts. Ces résultats, combinés à plusieurs études utilisateur spécifiques, débouchent sur l'identification d'un patron pour les interactions gestuelles de base incluant non seulement navigation (placement de caméra), mais aussi placement, rotation et mise à l'échelle des objets. Ce patron est étendu dans un second temps aux déformations complexes (ajout et suppression de matière ainsi que courbure ou torsion des objets, avec contrôle de la localité). Tout ceci nous permet de proposer et d'évaluer une interface d'édition des mondes 3D permettant une interaction tactile naturelle, pour laquelle le choix du mode (navigation, positionnement ou déformation) et des tâches correspondantes est automatiquement géré par le système en fonction du geste et de son contexte (sans menu ni boutons). Enfin, nous étendons cette interface pour y intégrer des déformations plus complexe à travers le transfert de vêtements d'un personnage à un autre, qui est étendu pour permettre la déformation interactive du vêtement lorsque le personnage qui le porte est déformé par interaction tactile.
Colonial Ambition and City Development: The Influence of Commissioners for the 1879 International Exhibition on Sydney's Architectural Identity (2008)

The second-half of the Nineteenth Century saw Sydney transformed from aconvict settlement into a commercial city. A representative cross-section of themen behind this transformation were appointed as Commissioners for the 1879Sydney International Exhibition: elites who were influential in all aspects ofcolonial activity, and who, in various capacities, advocated for the development ofthe city and drove the building of its public institutions, commercial premises andprivate dwellings. This paper attempts a brief characterisation of theCommissioners, connecting their occupations, interests and ambitions withSydneys urban development. They were educated in the classics andsubscribed to Old World views, whereas the International Exhibition they hostedtransmitted new ideologies that favoured utilitarianism and material progress andchallenged the classical basis of colonial taste. The tension between classicismand utilitarianism became evident in subsequent city development: there was anincreasing architectural eclecticism in Sydney as the century progressed.<br> </br>The methodology is based on careful archival research, but there are also somespeculations based on historical probability. What can be inferred as having beenthought by individuals is considered alongside what has been officially recordedor can be read from the urban fabric. Considering the voices of theCommissioners adds to the heritage significance of buildings; and understandingthe social, political and economic circumstances of their conception broadens thecultural significance of their place in the urban landscape. The Commissioners,by organising the Sydney International Exhibition, imposed their value systemsand world-views on the general population, promoting cultural and citydevelopment. They organised the Exhibition in their own image as a means ofshaping a better society. But the outcome was a society that, having benefitedfrom the Exhibition, continued to evolve and develop its own identity one thatpossibly differed from their expectations.
AN APPROACH TO TIME SERIES SMOOTHING AND FORECASTING USING THE EM ALGORITHM (1982)

Abstract. An approach to smoothing and forecasting for time series with missing observations is proposed. For an underlying state‐space model, the EM algorithm is used in conjunction with the conventional Kalman smoothed estimators to derive a simple recursive procedure for estimating the parameters by maximum likelihood. An example is given which involves smoothing and forecasting an economic series using the maximum likelihood estimators for the parameters.
Bounded expectations: resource analysis for probabilistic programs (2018)

This paper presents a new static analysis for deriving upper bounds on the expected resource consumption of probabilistic programs. The analysis is fully automatic and derives symbolic bounds that are multivariate polynomials in the inputs. The new technique combines manual state-of-the-art reasoning techniques for probabilistic programs with an effective method for automatic resource-bound analysis of deterministic programs. It can be seen as both, an extension of automatic amortized resource analysis (AARA) to probabilistic programs and an automation of manual reasoning for probabilistic programs that is based on weakest preconditions. An advantage of the technique is that it combines the clarity and compositionality of a weakest-precondition calculus with the efficient automation of AARA. As a result, bound inference can be reduced to off-the-shelf LP solving in many cases and automatically-derived bounds can be interactively extended with standard program logics if the automation fails. Building on existing work, the soundness of the analysis is proved with respect to an operational semantics that is based on Markov decision processes. The effectiveness of the technique is demonstrated with a prototype implementation that is used to automatically analyze 39 challenging probabilistic programs and randomized algorithms. Experiments indicate that the derived constant factors in the bounds are very precise and even optimal for some programs.
Immune Dysregulation and Tumor-Associated Gene Changes in Recurrent Respiratory Papillomatosis: A Paired Microarray Analysis (2008)

Recurrent respiratory papillomas (RRP) are benign airway tumors, caused primarily by human papillomaviruses (HPV) types 6 and 11. The disease is characterized by multiple recurrences after surgical removal, with limited effective therapy. To identify novel targets for future therapy, we established transcriptional profiles for actively growing papillomas compared with autologous, clinically normal, laryngeal epithelia (adjacent tissue). Total ribonucleic acid (RNA) from 12 papillomas and 12 adjacent tissues were analyzed by microarray, and the matched sets of tissues compared by paired t test, to identify differentially expressed genes in papilloma tissues while minimizing variations intrinsic to individual patients. Quantitative polymerase chain reaction (PCR) was used to confirm the relative expression levels for a subset of genes. Within the 109 differentially expressed transcripts whose expression varied at least three-fold were two large groups of genes with related functions. The first group consisted of 18 genes related to host defense, including both innate and adaptive immunity. The second group contained 37 genes that likely contribute to growth of papillomas as benign tumors, since the altered pattern of expression also had been reported previously in many cancers. Our results support our previous studies that document a systemic T(H)2-like adaptive immune response in RRP, and suggest that there is a role for altered innate immunity in RRP as well. We propose that HPV 6 and 11 infection establishes a tumorigenic microenvironment characterized by alteration of both innate inflammatory signals and adaptive immune responses that prevent effective T(H)1-like response, in conjunction with altered expression of numerous genes that regulate cellular growth and differentiation.
Advanced Development of a Helicopter Rotor Isolation System for Improved Reliability. Volume II. Detailed Report. (1977)

Abstract : This report includes the results of the analytical and experimental phases of a helicopter rotor isolation reliability and maintainability (R and M) program on a UH-1 helicopter, which was modified through the addition of a Dynamic Antiresonant Vibration Isolator (DAVI). The final flight test phase demonstrated that the DAVI-modified vehicle had substantially lower vibration levels than the standard vehicle. Vertically, the two-per-rev vibration level was reduced to less than one-fifth that of the standard vehicle through the transition speed range and less than one-half at high speeds. An R and M analysis indicated the Army could realize an annual cost savings of approximately $12,000,000 if 1000 Army UH-1Hs were equipped with DAVI isolation systems. This savings is predicated on the following assumptions: (1) vibration-induced failures will be reduced in proportion to the vibration reduction afforded by the DAVI isolation system, and (2) the UH-1Hs are utilized at the rate of 20 flight-hours per month.
Wave‐length dependencies of light scattering in normal and cold swollen rabbit corneas and their structural implications* (1973)

1. The studies described herein involve the use of light scattering measurements to characterize the ultrastructural arrangement of the constituent collagen fibrils in rabbit corneal stromas.2. Theoretical light scattering techniques for calculating the scattering to be expected from the structures revealed by electron micrographs are discussed, and comparison with the experimental light scattering tests the validity of these structures.3. The wave-length dependence of light transmission and of angular light scattering from normal corneas is in agreement with the short range ordering of collagen fibrils depicted in electron micrographs.4. The transmission measurements on oedematous rabbit corneas indicate that transmission decreases linearly with the ratio of thickness to normal thickness.5. The wave-length dependence of transmission through cold swollen corneas indicates that the increased scattering is caused by large inhomogeneities in the ultrastructure. Electron micrographs do, indeed, reveal the presence of such inhomogeneities in the form of large regions completely devoid of fibrils.