gebhart/patrepeval huggingface dataset:
Cloning and Thyroid Hormone Regulation of Albumin mRNA in<i>Rana catesbeiana</i>Tadpole Liver (1990)

Thyroid hormones are responsible for the specific biochemical and structural changes that occur during amphibian metamorphosis. In this study we screened a series of cDNAs from a library constructed from T4-treated premetamorphic tadpole liver poly(A)+ RNA in order to identify a clone that could be used to study the influence of T3 on liver-specific gene expression during Rana catesbeiana metamorphosis. The cDNA from one clone exhibited a greater degree of hybridization to liver RNA from thyroid hormone-treated tadpoles than untreated tadpoles and no hybridization to RNA from tail fins of tadpoles of either group. On Northern blots, the mRNA to which the cDNA hybridized was 2.3 kilobases in size. The pattern of hybridization to genomic DNA digested by various restriction enzymes was consistent with the presence of a single gene. Using slot blot analysis we found that the mRNA levels first rose above basal levels only after 5 days of immersion of tadpoles in 12.5 micrograms/liter T3. The mRNA levels increased approximately 10-fold after 7 and 9 days of treatment. Frog livers had mRNA levels that were intermediate between those in untreated tadpoles and tadpoles immersed in T3 for 7 days. Sequence analysis revealed a significant degree of homology to serum albumin and alpha-fetoprotein. While it is known that serum albumin levels rise dramatically during metamorphosis in Rana species, presumably playing a critical role in maintaining water and electrolyte balance during the animals' terrestrial phase, the molecular basis of the induction has not been fully explained.
Prospects for combined use of oncolytic viruses and CAR T-cells (2017)

With the approval of talimogene laherparepvec (T-VEC) for inoperable locally advanced or metastatic malignant melanoma in the USA and Europe, oncolytic virotherapy is now emerging as a viable therapeutic option for cancer patients. In parallel, following the favourable results of several clinical trials, adoptive cell transfer using chimeric antigen receptor (CAR)-redirected T-cells is anticipated to enter routine clinical practice for the management of chemotherapy-refractory B-cell malignancies. However, CAR T-cell therapy for patients with advanced solid tumours has proved far less successful. This Review draws upon recent advances in the design of novel oncolytic viruses and CAR T-cells and provides a comprehensive overview of the synergistic potential of combination oncolytic virotherapy with CAR T-cell adoptive cell transfer for the management of solid tumours, drawing particular attention to the methods by which recombinant oncolytic viruses may augment CAR T-cell trafficking into the tumour microenvironment, mitigate or reverse local immunosuppression and enhance CAR T-cell effector function and persistence.
Cytochrome <i>c</i> release and caspase activation in hydrogen peroxide‐ and tributyltin‐induced apoptosis (1998)

The ability of H2O2 and tributyltin (TBT) to trigger pro-caspase activation via export of cytochrome c from mitochondria to the cytoplasm was investigated. Treatment of Jurkat T lymphocytes with H2O2 resulted in the appearance of cytochrome c in the cytosol within 2 h. This was at least 1 h before caspase activation was observed. TBT caused cytochrome c release already after 5 min, followed by caspase activation within 1 h. Measurement of mitochondrial membrane potential (delta psi(m)) showed that both H2O2 and TBT dissipated delta psi(m), but with different time courses. TBT caused a concomitant loss of delta psi(m) and release of cytochrome c, whereas cytochrome c release and caspase activation preceded any apparent delta psi(m) loss in H2O2-treated cells. Thus, our results suggest that different mechanisms are involved in triggering cytochrome c release with these apoptosis-inducing agents.
Performance validation of a functionally distributed energy management architecture (1992)

The authors propose a functionally distributed computer (FDS) system for an energy management system (EMS) and a large-scale supervisory control and data acquisition (SCADA) system. The FDS consists of small-scale functional units executing simple functions and is characterized by both parallel processing and independence of subsystems. These units are mostly composed of microcomputers. The feasibility of such a system was investigated and results are reported. Such a system would improve performance, increase the flexibility of expansion, improve reliability, and reduce costs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Approximate geometric pattern matching under rigid motions (1999)

We present techniques for matching point-sets in two and three dimensions under rigid-body transformations. We prove bounds on the worst-case performance of these algorithms to be within a small constant factor of optimal and conduct experiments to show that the average performance of these matching algorithms is often better than that predicted by the worst-case bounds.
Comparison of biodegradable and metallic tension-band fixation for patella fractures: 38 patients followed for 2 years (1998)

We compared the outcome of patella fractures fixed by biodegradable tension-band (B) with self-reinforced polyglycolide or self-reinforced poly-L-lactide plugs and polyester ligaments or by metallic tension-band (M) with Kirschner wires and metallic cerclage wire in a randomized study. 38 fractures (18 with B and 20 with M) were treated. The follow-up time was 24 (14-32) months. The fractures healed in all patients after a medium of 8 weeks. In the B group, the clinical outcome was good in 13, fair in 4, and poor in 1 patient. In the M group, the corresponding figures were 15, 3 and 2. There were no clinical or radiographic differences between the two methods. Patella fractures can be treated, successfully using biodegradable tension-band fixation with no need for a second operation to remove the implants after bone union.
Voltage Imbalance Compensation for Doubly Fed Induction Generator Using Direct Resonant Feedback Regulator (2016)

This paper presents a resonant feedback regulator-based control strategy to compensate the point of common coupling (PCC) voltage imbalance for doubly fed induction generator (DFIG). The proposed control strategy utilizes a resonant compensator to directly generate the rotor voltage reference, rather than conventionally generating the negative sequence rotor current references. Thus, the sequence decomposition and DFIG parameter dependency can be avoided. Impacts of the resonant parameters and the network resistance/reactance (R/X) ratio on the control system performances, including the system stability, the rejection capability of the PCC voltage imbalance and the dynamic response of the control system, are theoretically analyzed. The experimental results validate the effectiveness of the proposed control strategy. The proposed voltage imbalance compensation strategy can be applied under any network R/X ratio condition.
Opportunities for the genetic manipulation of antioxidants in plant foods (1996)

Conference Article| August 01 1996 Opportunities for the genetic manipulation of antioxidants in plant foods P. M. Mullineaux; P. M. Mullineaux 1John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, U.K. Search for other works by this author on: This Site PubMed Google Scholar G. P. Creissen G. P. Creissen 1John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, U.K. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Received: March 18 1996 Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1996 Biochemical Society1996 Biochem Soc Trans (1996) 24 (3): 829–835. https://doi.org/10.1042/bst0240829 Article history Received: March 18 1996 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation P. M. Mullineaux, G. P. Creissen; Opportunities for the genetic manipulation of antioxidants in plant foods. Biochem Soc Trans 1 August 1996; 24 (3): 829–835. doi: https://doi.org/10.1042/bst0240829 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: γ-EC, γ-glutamylcysteine, GR, glutathione reductase, MDAR, monodehydroascorbate free radical reductase This content is only available as a PDF. © 1996 Biochemical Society1996 Article PDF first page preview Close Modal You do not currently have access to this content.
A New Family of Giardial Cysteine-Rich Non-VSP Protein Genes and a Novel Cyst Protein (2006)

Since the Giardia lamblia cyst wall is necessary for survival in the environment and host infection, we tested the hypothesis that it contains proteins other than the three known cyst wall proteins. Serial analysis of gene expression during growth and encystation revealed a gene, "HCNCp" (High Cysteine Non-variant Cyst protein), that was upregulated late in encystation, and that resembled the classic Giardia variable surface proteins (VSPs) that cover the trophozoite plasmalemma. HCNCp is 13.9% cysteine, with many "CxxC" tetrapeptide motifs and a transmembrane sequence near the C-terminus. However, HCNCp has multiple "CxC" motifs rarely found in VSPs, and does not localize to the trophozoite plasmalemma. Moreover, the HCNCp C-terminus differed from the canonical VSP signature. Full-length epitope-tagged HCNCp expressed under its own promoter was upregulated during encystation with highest expression in cysts, including 42 and 21 kDa C-terminal fragments. Tagged HCNCp targeted to the nuclear envelope in trophozoites, and co-localized with cyst proteins to encystation-specific secretory vesicles during encystation. HCNCp defined a novel trafficking pathway as it localized to the wall and body of cysts, while the cyst proteins were exclusively in the wall. Unlike VSPs, HCNCp is expressed in at least five giardial strains and four WB subclones expressing different VSPs. Bioinformatics identified 60 additional large high cysteine membrane proteins (HCMp) containing > or = 20 CxxC/CxC's lacking the VSP-specific C-terminal CRGKA. HCMp were absent or rare in other model or parasite genomes, except for Tetrahymena thermophila with 30. MEME analysis classified the 61 gHCMp genes into nine groups with similar internal motifs. Our data suggest that HCNCp is a novel invariant cyst protein belonging to a new HCMp family that is abundant in the Giardia genome. HCNCp and the other HCMp provide a rich source for developing parasite-specific diagnostic reagents, vaccine candidates, and subjects for further research into Giardia biology.
Sampling and Reconstructing Manifolds Using Alpha-Shapes (1997)

There is a growing interest for the problem of reconstructing the shape of an object from multiple range images. Several methods, based on heuristics, have been described in the literature. We propose the use of alpha-shapes, which allow us to give a formal characterization of the reconstruction problem and to prove that, when certain sampling requirements are satisfied, the reconstructed alpha-shape is homeomorphic to the original object and approximate it within a fixed error bound. In a companion paper, we describe practical methods to automatically select an optimal alpha value, to deal with less-than-ideal scans, and to fit smooth piecewise algebraic surface to the data points. 1 Introduction Cheaper, easier-to-use 3D digitizers are fostering a growing interest for the problem of shapereconstruction. Automatic methods for reconstructing an accurate geometric model of an object from a set of digital scans have applications in reverse engineering, shape analysis, virtual worlds aut...
Retinoids Induce Fibroblast Growth Factor-2 Production in Endothelial Cells via Retinoic Acid Receptor α Activation and Stimulate Angiogenesis In Vitro and In Vivo (2001)

The effect of retinoic acid (RA) on endothelial cells is still controversial and was examined in the present study. In bovine aortic endothelial cells (BAECs), all-trans RA (ATRA) and 9-cis RA (9CRA), but not 13-cis RA (13CRA), induced fibroblast growth factor-2 (FGF-2) production and exhibited a biphasic dose-dependent effect to enhance BAEC proliferation and differentiation into tubular structures on reconstituted basement membrane proteins (Matrigel); both processes were inhibited by FGF-2-neutralizing antibody. The pan RA receptor (RAR)-selective ligand (E)-4-[2-(5,5,8,8,-tetramethyl-5,6,7,8-tetrahydro-2-naphtalenyl)-1-propenyl] benzoic acid and the RARalpha-selective ligand 4-[1-(3,5,5,8,8-pentamethyl-5,6,7,8-tetrahydro-2-naphtyl)-ethenyl] benzoic acid stimulated the production of FGF-2, whereas the addition of the RARalpha-antagonist RO 41-5253 inhibited this effect. In BAECs, the forced expression of RARalpha, but not RARbeta or RARgamma, enhanced FGF-2 production, whereas the RARalpha-dominant negative, Delta403, blocked this effect. Furthermore, RARalpha overexpression directly stimulated BAEC differentiation on Matrigel and potentiated the effects of ATRA in this assay. Finally, ATRA-treated BAECs coinjected with Matrigel subcutaneously in mice induced neovascularization within the Matrigel plug, and ATRA also enhanced angiogenesis in the chicken chorioallantoic membrane assay. In conclusion, RA can stimulate endothelial cell proliferation and differentiation in vitro via enhanced RARalpha-dependent FGF-2 production, and it can also induce angiogenesis in vivo. The full text of this article is available at http://www.circresaha.org.
History of the MAA Since World War II (1967)

1. Preamble. Perhaps I may be permitted a personal remark which explains why I am so pleased to be given an opportunity to participate in this celebration: my father, a charter member of the Association, received his Ph.D. here at Cornell. I early came to know the mathematical names in Ithaca: Carver, Gillespie, Ranum, Silverman, Snyder, . . . and Hurwitz, who directed my father's thesis. It is a widely held opinion that mathematicians are especially attracted by two activities -music making and punning. It was Hurwitz, I have been told, who nicknamed a colleague with a reputation for making off with the magazines from the common room of Willard Straight: Procrastination, he called him, the thief of Time. The summer meeting just 20 years ago was cancelled because of the war, and the first big post-war meeting was here at Cornell in August of 1946, with 277 M\4AA members in attendance. It's interesting to note some of the names: W. D. Cairns of Oberlin; Newsom, also of Oberlin; Allendoerfer of Haverford (before Winger persuaded him to defect to the West); Carver, Hurwitz, Agnew, and Walker of Cornell; Feller, Jones, Kac, LeVeque, and Rosser, also of Cornell. It looks as though fewer than half of us stay put. But there were (and are) stalwarts, like Gehman, of Buffalo; McShane, of Virginia; Meder, of Rutgers; Tucker, of Princeton. The war was still with us in various ways: The Reverend J. T. O'Callahan of the College of the Holy Cross was awarded the Medal of Honor; the mathematics departments of several overseas colleges for servicemen were staffed by MAA members; the NRC offered fellowships to promising young people whose work had been interrupted by the war; the problems of rebuilding faculties, of allocating manpower, were pressing, especially in the face of droves of veterans returning to college under the G.I. bill. We were on the threshold of a new era, involving dramatic changes for the role of mathematics in our culture and of mathematicians in our society, and the MAA was bound to be deeply affected. I shall not attempt to chronicle the details of these changes, but rather to sort out some of the dominant features of the period. My notions of historiography have been influenced by Carlyle's On Heroes and Hero-Worship, and I shall continue with reference to personalities. Mathematics, after all, is made by mathematicians; and our Association bears the imprint of their interests and enthusiasms, their devotion and hard work, their prudence and logic, their emotions and prejudices. I can select only some examples and will of necessity omit acknowledgement of the equally important contributions of many others. One of our heroes is the embodiment of our virtues, although he might not appear heroic in other subcultures. I refer to Paul Erdos, whose contributions
Cross Plane Scattering Correction - Point Source Deconvolution In PET (2005)

transform to solve the deconvolution problem. However, using We propose a new point Source scattering deconvolution the Fourier transform limits the possibility Of COrRCting for the method (2-D). The cross-plane scattering is incorporated into position dependence of 2-D scattering. In our paper we intend to the algorithm by modelling a scattering point Source function. solve this problem and apply the 2-D method to the PET In the model, the scattering dependence both on axial and transaxial directions is reflected in the exponential fitting parameters and these parameters are directly estimated from a limited number of measured point response functions. Our results comparing the standard in-plane line source Scattering in PET is due to interactions of the 51IKeV deconvolution to our cross-plane point source deconvolution gamma rays with the medium, which causes the gamma rays to show that for a small source, the former technique change direction and energy. If we have a uniform medium overestimates the scatter fraction in the plane of the source and around a point source, the scattering will occur isotropically. A underestimate the scatter fraction in adjacent planes. In addition, point source scattering profile with an ideal detector system is we also propose a simple approximation technique for shown in Fig 1-a. The recorded 2-D scattering profile is deconvolution. projected onto the axial and transaxial directions as shown in Fig 1-b. Depending upon the distribution of activity and the medium, the scattering profile will vary.
Experimental validation of an intrasubject elastic registration algorithm for dynamic‐3D ultrasound images (2012)

The proposed feature-based registration technique has the potential for real-time implementation on a computationally inexpensive platform and has the capability of recovering nonrigid deformations in tissue with reasonable accuracy.
Structural studies of gel phases. Part 1.—Infrared spectroscopic study of silica monoliths; the effect of thermal history on structure (1991)

Fourier-transform mid-infrared (FTIR) and near-infrared (NIR) spectroscopies have been used to investigate the effects of thermal history on the bulk and surface structure of monolithic silica gels and glasses prepared by the sol–gel route. Effective dehydroxylation occurs above 700 °C with consolidation of the bulk structure leaving some residual silanol groups and water molecules within glasses treated above 1000 °C. Above this temperature, both functionalities are perturbed by the silica matrix and do not undergo rehydration. New assignments of bands in the first and second overtone regions have been made and used to assess silanol levels and functionality (first overtone region) and water levels (second overtone region).
Acyl protein thioesterase inhibitors as probes of dynamic<i>S</i>-palmitoylation (2013)

Protein palmitoylation describes the hydrophobic post-translational modification of cysteine residues in certain proteins, and is required for the spatial organization and composition of cellular membrane environments. Certain palmitoylated proteins are processed by acyl protein thioesterase (APT) enzymes, which catalyze thioester hydrolysis of palmitoylated cysteine residues. Inhibiting APT enzymes disrupts Ras trafficking and attenuates oncogenic growth signaling, highlighting these enzymes as potential therapeutic targets. As members of the serine hydrolase enzyme family, APT enzymes can be assayed by fluorophosphonate activity-based protein profiling (ABPP) methods, allowing rapid profiling of inhibitor selectivity and potency. In this review, we discuss recent progress in the development of potent and selective inhibitors to APT enzymes, including both competitive and non-competitive chemotypes. These examples highlight how ABPP methods integrate with medicinal chemistry for the discovery and optimization of inhibitors in complex proteomes.
Introduction (1997)

In the progress of any branch of science there are highlights of development and enlightenment, and signposts to future directions: these are usually recognised as single papers or series of papers in a scientific journal. The celebration of the first 50 years of publication of the British Journal of Pharmacology presented a golden opportunity to acknowledge some of the many such landmarks in the progress of pharmacology which have appeared in the Journal. This book contains a selection of facsimile reprints to represent papers that would be widely acknowledged to be among the long list of papers published in the Journal which have proved to have been significant. By that, it is meant that they have subsequently been widely cited, started a new area of interest, were a significant technical (method) advance, or were, in some way, a foundation stone in a pharmacological edifice (not necessarily the first publication on the topic, that may have been in another journal, but nearly the first). The selection ends at 1986 as the last 10 years seem to be too recent to assess yet by these criteria. The strict format of scientific publication usually conceals a more personal account of work; the passage of time allows the contribution to be seen in later years in the context of subsequent progress. So the opportunity also was taken to ask one of the original authors, or, where this was no longer possible, another appropriate pharmacologist, to write a Commentary to precede the facsimile of the paper. The commentators were invited to consider such aspects as the contemporary background to the stage of pharmacological knowledge at the time, how the idea for the work arose, the technical difficulties surmounted, the seeming impact (or perhaps lack of it) at the time, an assessment of the subsequent significance of the paper in terms of what it led to, and an assessment of how the development of the topic in later years relates to the paper. When David Brown was elected to succeed me as chairman of the Editorial Board he readily agreed to help me with this enterprise and was immediately an enthusiastic supporter of the project. Together, we expanded and refined the list of papers and commentators. I am greatly indebted to David for his support and expertise. Of course, the list of papers included is selective; we have had to leave out many other important papers, but we believe that those we have chosen will meet with general agreement. We thank Professor Tom Bolton for giving us access to his complete collection of the Journal (given to him by Professor Edith Bülbring) for the facsimile reproduction of the papers. I record my thanks to colleagues on the Executive Committee of the Editorial Board who encouraged me to go ahead with the project, especially to Nigel McNeil-Smith of Stockton Press for his expertise on production matters.