gebhart/patrepeval huggingface dataset:
Suppression of the Vacuolar Invertase Gene Prevents Cold-Induced Sweetening in Potato       (2010)

Potato (Solanum tuberosum) is the third most important food crop in the world. Potato tubers must be stored at cold temperatures to prevent sprouting, minimize disease losses, and supply consumers and the processing industry with high-quality tubers throughout the year. Unfortunately, cold storage triggers an accumulation of reducing sugars in tubers. High-temperature processing of these tubers results in dark-colored, bitter-tasting products. Such products also have elevated amounts of acrylamide, a neurotoxin and potential carcinogen. We demonstrate that silencing the potato vacuolar acid invertase gene VInv prevents reducing sugar accumulation in cold-stored tubers. Potato chips processed from VInv silencing lines showed a 15-fold acrylamide reduction and were light in color even when tubers were stored at 4°C. Comparable, low levels of VInv gene expression were observed in cold-stored tubers from wild potato germplasm stocks that are resistant to cold-induced sweetening. Thus, both processing quality and acrylamide problems in potato can be controlled effectively by suppression of the VInv gene through biotechnology or targeted breeding.
Lack of Insulin-Like Growth Factor I Exaggerates the Effect of Calcium Deficiency on Bone Accretion in Mice (2003)

Recent studies provide evidence that the GH/IGF-I axis plays a critical role in the regulation of bone accretion that occurs during puberty and that the peak bone mineral density (BMD) is dependent on the amount of dietary calcium intake during the active growth phases. To evaluate whether IGF-I deficiency exaggerates the effect of calcium deficiency on bone accretion during active growth phases, IGF-I knockout (KO) and wild-type (WT) mice were fed with low calcium (0.01%) or normal calcium (0.6%) for 2 wk during the pubertal growth phase and were labeled with tetracycline. The low calcium diet caused significant decreases in endosteal bone formation parameters and a much greater increase in the resorbing surface of both the endosteum and periosteum of the tibia of IGF-I KO mice compared with WT mice. Accordingly, femur BMD measured by dual energy x-ray absorptiometry or peripheral quantitative computed tomography increased significantly in IGF-I WT mice fed the low calcium diet, but not in IGF-I KO mice. IGF-I-deficient mice fed the normal calcium diet showed elevated PTH levels, decreased serum 1,25-dihydroxyvitamin D and serum calcium levels at baseline. Serum calcium changes due to calcium deficiency were greater in IGF-I KO mice compared with WT mice. PTH levels were 7-fold higher in IGF-I KO mice fed normal calcium compared with WT mice, which was further elevated in mice fed the low calcium diet. Treatment of IGF-I-deficient lit/lit mice with GH decreased the serum PTH level by 70% (P < 0.01). Based on these and past findings, we conclude that: 1) IGF-I deficiency exaggerates the negative effects of calcium deficiency on bone accretion; and 2) IGF-I deficiency may lead to 1,25-dihydroxyvitamin D deficiency and elevated PTH levels even under normal calcium diet.
Computational Geometry with the Rotating Calipers (1999)

The Rotating Calipers is a paradigm used to solve a number of problems in the field of Computational Geometry. The original algorithm was presented by Shamos in 1978 in order to find the diameter of a convex polygon; a few years later, Toussaint showed that the essence of the algorithm can be used to solve a number of other problems. In the past twenty years, research done by Toussaint and others has shown the Rotating Calipers to be a paradigm in Computational Geometry, applicable to a variety of problems. The resulting algorithms are often optimal in their time complexity, and always efficient and easy to implement.
Hair Transplantation Update (1998)

Hair transplantation is a time-tested, highly effective, permanent and natural method of improving male-pattern baldness and female alopecia. Improvements in technique continue to be developed, requiring one to keep abreast of changes in the field in order to provide patients with the best hair coverage possible while maintaining a natural appearance.
Advanced television systems for terrestrial broadcasting: Some problems and some proposed solutions (1995)

The first part of this paper discusses the requirements that must be met by a new television broadcasting system to maximize its acceptability to the various stakeholders, including broadcasters, equipment manufacturers, program producers, regulatory authorities, and viewers. The most important performance factors are efficient use of over-the-air spectrum, coverage versus quality, cost, interoperability, and the existence of a practical transition scenario. It is concluded that all receivers need not have the same peformance, and that low-cost receivers must be available for noncritical locations in the home. If this variation in price and performance is made possible by appropriate system design, then interoperability is facilitated and nondisruptive improvement over time is made possible, as desired by the Federal Communications Commission. In the second part of the paper, techniques that may permit meeting these requirements are discussed. These include joint multiresolution source and channel coding, multicarrier modulation, and hybrid analog/digital coding and transmission. The analog transform coefficients are subjected to spread-spectrum processing, and coded orthogonal frequency-division multiplex (COFDM) is applied to the complex hybrid symbols to be transmitted through the channel. Various methods of equalization and of improving noise, interference, and multipath rejection are compared. Finally, an example is given of a system that meets the various requirements by making use of a number of the techniques discussed. The system provides extended coverage, albeit at lower quality than currently proposed all-digital systems, and equal or higher quality than such systems in much of their service area. It also features self-optimization at each receiver depending on signal quality and receiver characteristics, and facilitates the design of receivers of lower cost and performance for less-critical applications.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
IL-7 stimulates protective immunity in mice against the intracellular pathogen, Toxoplasma gondii (1995)

Cytokines, in particular IFN-gamma and IL-12, are important in host protection against infection with Toxoplasma gondii. This parasite is a major cause of congenital infection and morbidity in immunosuppressed persons, especially those with AIDS. IL-7, a monomeric protein produced by bone marrow stromal cells and fetal thymus, is able to induce the proliferation of pro-B cells and CD4+ and CD8+ T cells, and to enhance cytotoxicity of CTL and NK cells. Inbred mice were infected with a lethal dose of T. gondii and given IL-7 twice daily. Mice treated with IL-7 beginning at the time of infection survived, whereas mice either treated after infection or not treated died. Phenotypic analysis of splenocytes identified an expansion of NK (asialo GM1+) cells and CD8+ T cell populations. In vivo depletion of NK (asialo GM1+) and CD8+ T cells showed that cells expressing these phenotypes were important for maintaining protection against the parasite. IFN-gamma depletion resulted in complete reversal of the protective effect of IL-7 administration. In vivo depletion of endogenous IL-7 enhanced susceptibility to infection. Cytokine analysis by semiquantitative reverse-transcriptase PCR showed that IL-7 enhances the IFN-gamma response and furthermore reverses the parasite-mediated down-regulatory response on IL-2. These observations indicate that exogenous administration of human rIL-7 is able to protect mice against acute parasite challenge by stimulating IFN-gamma production and augmenting the CD8+ T cell-mediated CTL response.
Multimode optical evanescent-wase acoustic sensor (1980)

An acoustic sensor using evanescent optical coupling between multimode optical fibers has been successfully demonstrated to have a detector shot-noise limited sensitivity at a sound pressure level of about 50 dB re 1 μPa/(Hz)12. The bandwidth of the sensor is dc to 8000 Hz. Construction of the device is described, along with a theoretical description of its operation compared to observed performance.
Batch-processed vacuum-sealed capacitive pressure sensors (2001)

This paper reports two multitransducer vacuum-sealed capacitive barometric pressure sensors, one using single-lead and the other using multiple-leads to transfer the electrical signal out of the vacuum-sealed reference cavity. The first device operates with a resolution of 37 mtorr over a pressure range from 600 to 800 torr. The sensitivity is 27 fF/torr (3000 ppm/torr). The TCO at 750 torr is 3900 ppm//spl deg/C and the TCS is 1000 ppm//spl deg/C. The second device has a resolution of 25 mtorr over a range from 500 to 800 torr, with individual transducer sensitivity of 39 fF/torr. The TCO at 750 torr is 1350 ppm//spl deg/C and TCS is 1000 ppm//spl deg/C. Both devices have an on-chip compensation capacitor and are read out using an electronically-trimmed switched-capacitor charge integrator.
Hif-1α regulates differentiation of limb bud mesenchyme and joint development (2007)

Recent evidence suggests that low oxygen tension (hypoxia) may control fetal development and differentiation. A crucial mediator of the adaptive response of cells to hypoxia is the transcription factor Hif-1alpha. In this study, we provide evidence that mesenchymal condensations that give origin to endochondral bones are hypoxic during fetal development, and we demonstrate that Hif-1alpha is expressed and transcriptionally active in limb bud mesenchyme and in mesenchymal condensations. To investigate the role of Hif-1alpha in mesenchymal condensations and in early chondrogenesis, we conditionally inactivated Hif-1alpha in limb bud mesenchyme using a Prx1 promoter-driven Cre transgenic mouse. Conditional knockout of Hif-1alpha in limb bud mesenchyme does not impair mesenchyme condensation, but alters the formation of the cartilaginous primordia. Late hypertrophic differentiation is also affected as a result of the delay in early chondrogenesis. In addition, mutant mice show a striking impairment of joint development. Our study demonstrates a crucial, and previously unrecognized, role of Hif-1alpha in early chondrogenesis and joint formation.
Turbulent drag reduction using compliant surfaces (1997)

Over the past forty years intensive investigations into the use of compliant surfaces have been undertaken, both theoretically and experimentally, in order to obtain turbulent drag reduction in boundary–layer flows. Although positive results were found in some of the studies, none of these had been successfully validated by independent researchers. In this paper the results are reported of a recent investigation carried out by the authors to verify the experimental results of Semenov in 1991 and Kulik and co–workers in 1991, who successfully demonstrated the ability of compliant surfaces to reduce the skin–friction drag and surface–flow noise in a turbulent boundary layer. A strain–gauge force balance was used in the present study to directly measure the turbulent skin–friction drag of a slender body of revolution in a water tunnel. Changes in the structure of turbulent boundary layer over a compliant surface in comparison with that over a rigid surface were also examined. The results clearly demonstrate that the turbulent skin friction is reduced for one of the two compliant coatings tested, indicating a drag reduction of up to 7 per cent within the entire speed range of the tests. The intensities of skin–friction and wall–pressure fluctuations measured immediately downstream from the compliant coating show reductions in the intensities of up to 7 and 19 per cent, respectively. The results also indicate reductions in turbulence intensity by up to 5 per cent across almost the entire boundary layer. Furthermore, an upwards shift of the logarithmic velocity profile is also evident indicating that the thickness of the viscous sublayer is increased as a result of turbulent drag reduction due to the compliant coating. It is considered that the results of the present experimental investigation convincingly demonstrate for the first time since the earlier work in Russia (by Semenov and Kulik) that a compliant surface can indeed produce turbulent drag reduction in boundary–layer flows.
AWS certified solutions architect official study guide: associate exam (2017)

This is your opportunity to take the next step in your career by expanding and validating your skills on the AWS cloud. AWS has been the frontrunner in cloud computing products and services, and the AWS Certified Solutions Architect Official Study Guide for the Associate exam will get you fully prepared through expert content, and real-world knowledge, key exam essentials, chapter review questions, access to Sybex's interactive online learning environment, and much more. This official study guide, written by AWS experts, covers exam concepts, and provides key review on exam topics, including: * Mapping Multi-Tier Architectures to AWS Services, such as web/app servers, firewalls, caches and load balancers * Understanding managed RDBMS through AWS RDS (MySQL, Oracle, SQL Server, Postgres, Aurora) * Understanding Loose Coupling and Stateless Systems * Comparing Different Consistency Models in AWS Services * Understanding how AWS CloudFront can make your application more cost efficient, faster and secure * Implementing Route tables, Access Control Lists, Firewalls, NAT, and DNS * Applying AWS Security Features along with traditional Information and Application Security * Using Compute, Networking, Storage, and Database AWS services * Architecting Large Scale Distributed Systems * Understanding of Elasticity and Scalability Concepts * Understanding of Network Technologies Relating to AWS * Deploying and Managing Services with tools such as CloudFormation, OpsWorks and Elastic Beanstalk. Learn from the AWS subject-matter experts, review with proven study tools, and apply real-world scenarios. If you are looking to take the AWS Certified Solutions Architect Associate exam, this guide is what you need for comprehensive content and robust study tools that will help you gain the edge on exam day and throughout your career.
Aromatic Polyamidines Inhibiting the Tat-Induced HIV-1 Transcription Recognize Structured TAR-RNA (2001)

We have investigated the effects of aromatic polyamidines on HIV-1 transcription. We found a block to Tat-induced HIV-1 transcription assessed by inhibition of CAT activity in HL3T1 cells at a concentration lower than the IC50 value, suggesting that molecules with three (TAPB) and four (TAPP) benzamidine rings could be useful against HIV-1. In contrast, aromatic polyamidines with only two benzamidine rings (DAPP) did not block Tat-induced transcription. We reasoned that this effect could be due to binding of TAPB and TAPP to HIV-1 TAR RNA. By EMSA and filter binding assays, we studied possible interactions of aromatic polyamidines with HIV-1 TAR RNA. Wild-type TAR RNA or TAR RNA with mutations in the stem or bulge sequences, but retaining the stem-loop structure, was used to define the RNA-binding activities of these compounds. Our data suggest that aromatic polyamidines with two (DAPP) and four (TAPP) benzamidine rings, respectively, do not bind to TAR RNA or bind without sequence selectivity. Interestingly, an aromatic polyamidine with three benzamidine rings (TAPB) recognizes the wild-type TAR RNA in a specific manner. Furthermore, we found that introduction of one halogen atom into the benzamidine rings strongly increases the RNA-binding activity of these compounds.
[Oxidative refolding of proteins]. (2003)

The mechanism of oxidative refolding of proteins was elucidated in more detail from the intensive and extensive studies in the past decades. 1. Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory. 2. It is the native interactions rather than the non-native interactions that direct the folding process. This is not necessarily incompatible with the importance of the non-native disulfide intermediates in the bovine pancreatic trypsin inhibitor (BPTI) pathway, which are just a chemical necessity in the intramolecular arrangement to facilitate native disulfide formation. 3. Based on the BPTI refolding it was suggested that disulfide bonds have a stabilizing effect on the native state without determining either the folding pathway or the final three-dimensional structure of the protein. This point of view is not applicable to other proteins. Studies on the refolding of prochymosin unequivocally demonstrated that the formation of native disulfides is the prerequisite to the recovery of the native conformation. It is more likely that the interdependence between the native disulfide formation and the formation of native structure is a general rule. 4. At the early stage of oxidative refolding disulfide formation is essentially a random process, with the progress of refolding further disulfide formation is increasingly dependent on the conformations of the intermediates. Enhancing the renaturation yield of recombinant proteins is a major challenge in biotechnology. In addition to aggregation, the formation of species with mispaired disulfide bonds is a leading cause of decreased yield. Progress in understanding the mechanism of oxidative refolding has provided insight into how to solve this problem. As described above, at the later stage of refolding disulfide formation depends on the conformations of intermediates. The intermediates with native-like and flexible structure favourable for native disulfide formation and correct refolding are productive intermediates, while the unproductive intermediates tend to adopt stable conformations, which render the thiol groups and disulfide bond(s) inaccessible and further folding unfavourable energetically. Therefore, the principle to enhance the renaturation yield of disulfide-containing proteins is to cause the productive intermediates to predominate by destabilizing the unproductive intermediates. To approach this, alkaline pH, low temperature, labilizing agents, protein disulfide isomerase and its analogues and alteration of primary structure have been proved useful to adjusting the structure of the unproductive intermediates so as to facilitate thiol/disulfide interchange and in turn the native disulfide formation. The prospects for the oxidative refolding of proteins both in basic and applied researches are discussed in this review article.
Diagnostic applications of monoclonal antibodies to human cancer. (1984)

Monoclonal antibodies elicited to surface antigens on a wide variety of normal and neoplastic cell types have been used with increasing frequency in the diagnosis and classification of cancer. Their widest application so far has been in the in vitro analysis of leukemia and lymphoma using blood or bone marrow samples or tissue biopsy specimens. Although no true leukemia-specific marker has been found, the complex biologic nature of this group of tumors has been shown by monoclonal antibody studies. More recently, these antibodies were produced against solid tumor-associated antigens. The antibodies have helped to identify the tissue of origin of malignant neoplasms and, in some cases, to monitor the course of disease. Carefully chosen panels of monoclonal antibodies will become valuable additions to the diagnostic pathologist's arsenal.
Failure-Tolerant Transaction Routing at Large Scale (2010)

Emerging Web2.0 applications such as virtual worlds or social networking websites strongly differ from usual OLTP applications. First, the transactions are encapsulated in an API such that it is possible to know which data a transaction will access, before processing it. Second, the simultaneous transactions are very often commutative since they access distinct data. Anticipating that the workload of such applications will quickly reach thousands of transactions per seconds, we envision a novel solution that would allow these applications to scale-up without the need to buy expensive resources at a data center. To this end, databases are replicated over a P2P infrastructure for achieving high availability and fast transaction processing thanks to parallelism. However, achieving both fast and consistent data access on such architectures is challenging at many points. In particular, centralized control is prohibited because of its vulnerability and lack of efficiency at large scale. Moreover dynamic behavior of nodes, which can join and leave the system at anytime and frequently, can compromise mutual consistency. In this article, we propose a failure-tolerant solution for the distributed control of transaction routing in a large scale network. We leverage a fully distributed approach relying on a DHT to handle routing metadata, with a suitable failure management mechanism that handles nodes dynamicity and nodes failures. Moreover, we demonstrate the feasibility of our transaction routing implementation through experimentation and the effectiveness of our failure management approach through simulation.
Spatiotemporal Control of Migration of Single Cells on a Photoactivatable Cell Microarray (2007)

Spatiotemporal control of cell migration was achieved on a photoactivatable cell-culturing substrate. Single cells were micropatterned on the substrate and were induced to extend protrusions led by lamellipodia or filopodia alternatively by the subsequent formation of wide or narrow paths in their surroundings, respectively. By tracking the migration of single cells in a microarray format, we performed quantitative analysis of the migration rates of single cells.
Inhibition of the metabolism of the colon carcinogen, azoxymethane, by pyrazole. (1978)

Abstract Alterations of [ 14 C]azoxymethane metabolism by pretreatment with pyrazole, an inhibitor of alcohol dehydrogenase, were studied in male F344 rats. Whereas control animals exhaled approximately 36% of the [ 14 C]azoxymethane dose (10.5 mg/kg) in 6 hr as 14 CO 2 , rats pretreated with pyrazole (40 mg/kg) exhaled approximately 3.5%. Pretreatment with pyrazole (360 mg/kg) caused a complete inhibition of exhaled 14 CO 2 for at least 6 hr. After 48 hr the amount of exhaled 14 CO 2 comprised approximately 50% of the dose in the controls, and 26 and 20% for the 40- and 360-mg/kg pyrazole-pretreated rats, respectively. [ 14 C]Methylamine, [ 14 C]urea, and [ 14 C]methylazoxymethanol were detected in the urines of control rats. Pretreatment with pyrazole (40 mg/kg) caused a 2.5-fold increase in urinary methylazoxymethanol; pretreatment with pyrazole (360 mg/kg) increased the urinary level of azoxymethane some 2.5- to 3-fold. In contrast to controls and 40-mg/kg pyrazole-pretreated rats, 360-mg/kg pyrazole-pretreated animals showed increased urinary excretion of [ 14 C]methylamine and continued excretion of [ 14 C]methylazoxymethanol and unmetabolized [ 14 C]azoxymethane during the 24- to 48-hr period after [ 14 C]azoxymethane administration. Washed liver microsomes from pyrazole-treated rats converted azoxymethane to methylazoxymethanol at the same rate as controls, but the hydroxylation could be inhibited by the addition of pyrazole to the incubation system. These results show that pyrazole inhibits the metabolism of azoxymethane at at least two steps. At 40 mg/kg, pyrazole inhibits the oxidation of methylazoxymethanol, presumably by blocking alcohol dehydrogenase. At 360 mg/kg, pyrazole blocks this reaction and inhibits the hydroxylation of azoxymethane.