gebhart/patrepeval huggingface dataset:
Glucocorticoid inhibition of lymphokine secretion by alloreactive T lymphocyte clones. (1984)

The effect of glucocorticoids on lymphokine production by T lymphocytes was examined by using long-term alloreactive T cell clones that secreted one or more of the lymphokines interleukin 2 (IL 2), interferon-gamma, macrophage-activating factor (MAF), and colony-stimulating factor when stimulated by an antigen or a mitogen. Production of all of these four lymphokines was inhibited when glucocorticoids were added at physiologic concentrations (10(-8) to 10(-6) M) to clones stimulated with concanavalin A (Con A). Clones were heterogeneous with respect to their sensitivity to glucocorticoid inhibition of MAF production; cytolytic clones were generally more resistant than noncytolytic clones. The glucocorticoid dexamethasone (Dex) and an IL 2-containing supernatant exerted opposing effects on clonal MAF production. Kinetics experiments showed that Dex inhibited MAF production by reducing the rate of secretion without causing a compensatory increase in the duration of secretion, whereas the IL 2 source increased the rate and the total amount of MAF secretion. Dex abrogated the effect of IL 2. Inhibition by Dex was apparent from the earliest time of detectable MAF production (about 4 hr after stimulation) and increased with longer exposure until production ceased (12 to 24 hr). Pre-exposure and removal of Dex before Con A stimulation also inhibited MAF release. Effects of Dex on lymphokine secretion by clones could be dissociated from effects on their growth in response to stimulator cells and IL 2. Factor production by the 16 clones tested was inhibited to some degree. Proliferation, however, by two of these clones (both cytolytic) was unaffected by Dex, whereas proliferation of two noncytolytic clones was strongly inhibited even in the presence of a saturating dose of IL 2.
SARS-CoV-2–specific T cells are rapidly expanded for therapeutic use and target conserved regions of the membrane protein (2020)

T-cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been described in recovered patients, and may be important for immunity following infection and vaccination as well as for the development of an adoptive immunotherapy for the treatment of immunocompromised individuals. In this report, we demonstrate that SARS-CoV-2-specific T cells can be expanded from convalescent donors and recognize immunodominant viral epitopes in conserved regions of membrane, spike, and nucleocapsid. Following in vitro expansion using a good manufacturing practice-compliant methodology (designed to allow the rapid translation of this novel SARS-CoV-2 T-cell therapy to the clinic), membrane, spike, and nucleocapsid peptides elicited interferon-γ production, in 27 (59%), 12 (26%), and 10 (22%) convalescent donors (respectively), as well as in 2 of 15 unexposed controls. We identified multiple polyfunctional CD4-restricted T-cell epitopes within a highly conserved region of membrane protein, which induced polyfunctional T-cell responses, which may be critical for the development of effective vaccine and T-cell therapies. Hence, our study shows that SARS-CoV-2 directed T-cell immunotherapy targeting structural proteins, most importantly membrane protein, should be feasible for the prevention or early treatment of SARS-CoV-2 infection in immunocompromised patients with blood disorders or after bone marrow transplantation to achieve antiviral control while mitigating uncontrolled inflammation.
Plasma formation of buffer layers for multilayer resist structures (1981)

Recently, Li and Richards described a plasma treatment which may be used to prevent intermixing of successive layers of positive resists. We have studied the characteristics of the fluorinated polymer layers produced by this process, and have used a modification of the process to perform multilayer lithography for device fabrication.
A Novel Drive Strategy for PMSM Compressor (2010)

Based on the analysis of compressor load behaviors, a new three-phase startup strategy for permanent magnet synchronous motor (PMSM) compressor is presented, which includes parking, open-loop startup and closed-loop operation. When the speed loop closes, an improved method of flux estimation for PMSM compressor sensorless control is proposed, which accomplishes parking, efficient speed estimation and disturbance eliminating, it is caused by integrating element of back electromotive force (BEMF). A laboratory prototype has been fabricated using this strategy and system analysis, measured experimental results are presented.
Darstellung und spektroskopische Eigenschaften von Alkoxyaminosilanen (RO)<sub>n</sub>Me<sub>3–n</sub>SiNHC<sub>6</sub>H<sub>4</sub>X (1983)

Abstract Durch Reaktion von Chlorsilanen mit Anilin‐Derivaten und durch Umaminierung von Silylaminen wurden Alkoxyaminosilane der Reihen (RO) n Me 3–n SiNHC 6 H 4 X, n = 0, 1, 2, 3, darunter 22 neue Verbindungen, erhalten. Ihre IR‐ und 1 H‐NMR‐Spektren wurden vermessen, die NH‐Aciditäten ermittelt und der Einfluß der variablen Polarisierbarkeitseffekte der (RO) n Me 3‐n Si‐Gruppierung auf die Hammettschen Beziehungen von Δ v und δ der NH‐Protonen diskutiert.
A feature‐based approach to facial expression cloning (2005)

Abstract In this paper, we present a feature‐based approach to cloning facial expressions from an input face model to an output model, using predefined source key‐models and the corresponding target key‐models. Adopting a scattered data interpolation technique, our approach consists of two parts: analysis of face key‐models and synthesis of facial expressions. In the analysis part carried out once at the beginning, key‐models are segmented automatically into five regions, each containing one of five facial features, that is, eyes, cheeks, and the mouth, which give rise to five sets of source key‐shapes and the corresponding sets of target key‐shapes. Using the key‐shapes of each source feature, those of the corresponding target feature are parameterized. In the synthesis part, given a sequence of face models comprising an input animation, five output features are obtained separately by blending their own target key‐shapes. These separately produced features are combined to synthesize the output face model at each frame. Our feature‐based approach enables cloning of diverse expressions including asymmetric ones convincingly with a small number of face key‐models while exhibiting an on‐line, real‐time performance. Copyright © 2005 John Wiley & Sons, Ltd.
Use of Monoclonal Antibodies To Identify Serotypes of Enterovirus Isolates (1998)

Nonpoliovirus enteroviruses cause a variety of diseases that are common in young children and adults. The "gold standard" for laboratory diagnosis of enteroviruses is cell culture isolation, followed by serotype identification by neutralization assay. These procedures are time-consuming and expensive. Rapid serotype identification of enteroviruses is important in differentiating nonpoliovirus enterovirus pathogens from vaccine strain polioviruses that can be shed for some time after vaccination. In the present investigation, we evaluated a rapid method for serotype identification of enteroviruses by indirect immunofluorescence assay (IFA) using commercially available monoclonal antibodies for polioviruses, coxsackieviruses type B, and six serotypes of commonly circulating echoviruses. Of 291 isolates of enteroviruses included in the study, 95 were polioviruses and 196 were nonpoliovirus enteroviruses. Two hundred thirty-four of these (38 polioviruses and 196 nonpoliovirus enteroviruses) were consecutively grown in the laboratory over a 5-year period. IFA identified the serotypes of 74% of the consecutive isolates and 71% of all enterovirus isolates by yielding a positive staining result. The levels of agreement in the identification of the enterovirus group between IFA and neutralization tests were 92% for consecutively grown isolates and 85% for all enterovirus isolates. The sensitivity of the IFA for the detection of viruses for which specific monoclonal antibodies were applied was 73% for polioviruses, 85% for coxsackieviruses type B, and 94% for echoviruses. Specificity was near 100% for polioviruses and coxsackieviruses type B and 94% for echoviruses. We conclude that IFA can be helpful as a preliminary test for serotype identification of enteroviruses. The results are most accurate when the test identifies the isolate as a poliovirus.
Real-Time Droplet DNA Amplification with a New Tablet Platform (2012)

We present a novel droplet-based tablet platform for temporal polymerase chain reaction (PCR) in microliter droplets. The simple design of the device does not require extensive processing or external equipment, which allows for greater ease of use and integration as a point-of-care diagnostic. We demonstrate its functionality to perform both PCR and reverse-transcription PCR for λ phage DNA and H3 influenza RNA with ramp rates and cycle times consistent with traditional PCR thermal cyclers. We additionally investigate the effect of performing PCR in small volumes on the reaction performance by specifically examining adsorption of reagents at the oil/water interface. We determined that adsorption of Taq polymerase at the biphasic interface reduces yield and impairs reaction performance at standard concentrations. Thus, microdroplet PCR reactions require additional polymerase to achieve sufficient amplification and we project for applications utilizing nanodroplets or picodroplets like digital applications, even greater concentrations of polymerase are required to achieve desired results. Following the adsorption investigation, we evaluated the sensitivity of λ phage PCR on our platform to be less than 2.0 copies/μL with an efficiency of 104.4% and similar sensitivity for reverse-transcription PCR for influenza H3 RNA.
Longitudinal Ion Oscillations in a Hot Plasma (1961)

Linearized, longitudinal waves in a hot plasma include, besides the familiar electron plasma oscillations, in which the frequency ω is of order ωp = (4πne2/m)½, also ion plasma oscillations with ω ≈ ωp(m/M)½. The properties of the latter are explored using a Vlasov equation description of the plasma. For equal ion and electron temperatures, Te = Ti, there exists a discrete sequence of ion oscillations, but all are strongly damped, i.e., have -Im ω/Re ω ⪞ 0.5, and hence are not likely to be observable. The ratio Im ω/Re ω can be made to approach zero (facilitating detection of the waves) by either increasing Te/Ti or by producing a current flow in the plasma. In the latter case, Im ω can even be made positive (corresponding to growing waves), the current required for this being smaller the larger the value of Te/Ti. This growing wave is just the familiar two-stream instability which is thus seen to be an unstable ion oscillation. It is also noteworthy that the ion oscillations, which for small k have the properties usually associated with an acoustic wave (longitudinal polarization, ω ∝ k), are obtained using a formalism which is sometimes designated as ``collisionless.''
Exploring the Performance Fluctuations of HPC Workloads on Clouds (2010)

Clouds enable novel execution modes often supported by advanced capabilities such as autonomic schedulers. These capabilities are predicated upon an accurate estimation and calculation of runtimes on a given infrastructure. Using a well understood high-performance computing workload, we find strong fluctuations from the mean performance on EC2 and Eucalyptus-based cloud systems. Our analysis eliminates variations in IO and computational times as possible causes, we find that variations in communication times account for the bulk of the experiment-to-experiment fluctuations of the performance.
Serotonin uptake in blood platelets of psychiatric patients (1982)

• Platelet serotonin (5-HT) uptake was determined in 72 newly admitted, unmedicated psychiatric patients. Decreased maximum velocity (V max ) of 5-HT uptake was present in unipolar and bipolar depressed patients as well as schizoaffective depressed patients. The apparent Michaelis constant (k m ) of 5-HT uptake was normal in these groups, as was V max and K m in manicdepressive and chronic schizophrenic patients. Treatment of depressed patients with notriptyline hydrochloride or imipramine hydrochloride increased K m significantly. There was a trend for the increase in K m in the nortriptyline-treated patients to correlate with clinical improvement. Decreased 5-HT uptake in platelets provides additional evidence for the role of 5-HT in the pathophysiologic process of some forms of depression.
Suspension polymerization of acrylamide in an oscillatory baffled reactor: From drops to particles (2001)

Abstract Droplet and particle‐size distribution of inverse phase suspension polymerization of acrylamide was studied in an oscillatory baffled reactor (OBR). A discretized population balance model developed estimates the coalescence droplet rate and the type of droplet interactions contributing to the coalescence process in the system. In an OBR, fluid mixing is achieved by eddies generated when a set of prespecified orifice baffles moves periodically through liquid. These vortices can be controlled by a combination of geometrical and operational parameters, such as orifice diameter, baffle spacing, oscillation frequency, and oscillation amplitude. This type of mixing, combining with a close‐to‐constant level of turbulence intensity in the reactor, has led to a high degree of controllability and repeatability for both droplet and particle size, and a fine particle level significantly lower than a conventional stirred‐tank reactor. Generic correlation is established linking the final bead size and Sauter mean droplet size with power dissipation.
Binding and functional properties of doxazosin in the human prostate adenoma and canine brain (1990)

The binding and functional properties of doxazosin were characterized in the canine brain and human prostate. 3H-Doxazosin binding sites were characterized in canine brain and human prostate homogenates using saturation experiments. The binding of 3H-doxazosin in the canine brain was consistently saturable and of high affinity. The mean equilibrium dissociation constant (Kd) and density (Bmax) of 3H-doxazosin binding sites in the canine brain were 0.19 nM and 2.17 fmol/mg wet wt, respectively. The binding of 3H-doxazosin in human prostate homogenates was not consistently linear owing to a relatively high proportion of nonspecific doxazosin binding sites. The mean Kd and Bmax of 3H-doxazosin binding sites in the prostate determined from the saturation experiments yielding linear Scatchard plots were 0.2 nM and 0.51 fmol/mg wet wt. The pharmacology of doxazosin binding sites was further characterized in the canine brain using competitive binding experiments. The rank order of IC50corr values for norepinephrine, clonidine, yohimbine, terazosin, and prazosin indicated that doxazosin binds selectively to alpha 1 and alpha 2 adrenergic binding sites. The relative affinity of unlabeled doxazosin for alpha 1 and alpha 2 binding sites in the human prostate was determined by displacing 125I-Heat or 3H-rauwolscine with varying concentrations of unlabeled doxazosin. The affinity of doxazosin for alpha 1 binding sites in the prostate adenoma was approximately 100-fold greater than its affinity for alpha 2 binding sites. The potency of doxazosin for inhibiting phenylephrine-induced contractions in the prostate indicated that prostate smooth muscle contraction is mediated by alpha 1 adrenoceptors.
Plan-Per-Tuple Optimization Solution - Parallel Execution of Expensive User-Defined Functions (1998)

Object-Relational database systems allow users to define new user-defined types and functions. This presents new optimizer and run-time challenges to the database system on shared-nothing architectures. In this paper, we describe a new strategy we are exploring for the NCR Teradata Multimedia Database System; our focus is directing research for real applications we are seeing. In doing so, we will briefly describe optimizer challenges particularly related to predicate use of large multimedia objects, such as video/audio clips, images, and text documents. The motivation for this work is based on database tuning [SD961 for diverse queries related to multimedia objects. Most notably, expensive and/or high variant user defined functions [He198]. Our approach is referred to as plan-per-tuple. The
Enhancement of Catalytic Properties for the Electrooxidation of Polyols on Bi-Modified Pt and Pd Nanoparticles (2012)

Abstract not Available.
DENV-2 subunit proteins fused to CR2 receptor-binding domain (P28)-induces specific and neutralizing antibodies to the Dengue virus in mice (2013)

Domain III (DIII) of the dengue virus (DENV) envelope (E) protein induces strong neutralizing type-specific antibodies. In addition, a region near the fusion loop in domain II (DII) induces the production of cross-reactive antibodies with neutralizing potential. Thus, this study aimed to generate DENV-2 recombinant fusion proteins (i.e., rEII*EIII and rEII*EIII/NS1*) either alone or fused to 3 copies of P28, the minimum CR2-binding domain of the complement protein C3d. The 4 recombinant proteins were generated in a Drosophila melanogaster Schneider 2 (S2) cell system. The expression and secretion of the recombinant proteins were confirmed in vitro using immunofluorescence (IF) and western blot (WB) analyses. Human dengue immune serum samples recognized recombinant proteins. The immunogenicity of the 4 proteins in BALB/c mice was analyzed using ELISA, and the results revealed that the induced specific antibody response was higher in the groups of mice immunized with the P28 fusion proteins. Interestingly, although the 4 recombinant proteins were able to elicit high levels of neutralizing antibodies in BALB/c mice; no adjuvant effect was observed in terms of neutralizing antibodies in the groups immunized with proteins containing P28. Thus, ELISA and PRNT50 assays may evaluate different epitopes and responses, where ELISA showed a wider response that did not always correlate with neutralization. Furthermore, the elicited antibodies were able to recognize the immobilized E glycoprotein of DENV. All mice vaccinated with the DENV-2 recombinant proteins showed induction of higher levels of IgG1 antibodies than of IgG2a antibodies.
Ccalf Coefficient Derivation Using Combined Neighbors (2020)

Loop filtering is widely used in recent video coding standards to reduce coding artifacts introduced by prediction and residual coding process. Versatile Video Coding (VVC) standard employs Cross Component Adaptive Loop Filter (CCALF) to improve chroma fidelity. This paper describes CCALF coefficient derivation that uses combined luma neighboring pixels. With the proposed combination, more correlated pixels provide less contribution to CCALF process resulting in improving performance. Simulation results show the proposed method has combined BD-rate gains of -1.51%, -2.01%, -2.40% for all intra (AI), random Fig. 1: Block diagram of VVC showing interactions between access (RA), and low delay (LB) conditions, respectively, CCALF and other loop filtering tools [1] compared to VTM-7.0 reference software.
Holographic characterization of epoxy resins at 351.1 nm (1998)

The optical characteristics of two commercially available UV curable epoxy resins are investigated using nondegenerate four-wave mixing. The materials assessed are optimized for use with a UV argonion laser. The holographic gratings were written at a wavelength of ? 5351.1 nm for an irradiance range 0.5 to 3.0 W/cm2 and read at ? 5632.8 nm to compare the reactivity, curing speed, shrinkage, and resolution of the resins. These experiments were carried out to prove the suitability of the photopolymerization systems for microstereolithography.
Inactivation of the p16 (INK4A) tumor-suppressor gene in pancreatic duct lesions: loss of intranuclear expression. (1998)

Pancreatic adenocarcinoma develops from histologically identifiable intraductal lesions that undergo a series of architectural, cytological, and genetic changes. Limited genetic evidence recently suggested that the p16 gene plays a role in the progression of these "duct lesions." Duct lesions were identified in pancreata from 33 pancreaticoduodenectomies performed for infiltrating adenocarcinoma. All of these infiltrating adenocarcinomas were previously shown to contain alterations in the p16 gene or its promoter. Monoclonal and polyclonal anti-p16 antibodies were used for histological immunodetection. One hundred twenty-six duct lesions were identified. Nine (30%) of 30 flat, 4 (27%) of 15 papillary, 37 (55%) of 67 papillary with atypia, and 10 (71%) of 14 carcinoma in situ duct lesions showed loss of p16 expression. These included 30% of the flat lesions versus 53% of the nonflat lesions and 29% of the nonatypical lesions versus 58% of the atypical lesions. For both comparisons, the differences were statistically significant (P = 0.036 and P = 0.003, respectively). Loss of p16 expression occurs more frequently, but not exclusively, in higher-grade duct lesions. These data support the hypothesis that pancreatic duct lesions are neoplastic and that they represent the precursors of infiltrating adenocarcinoma. Immunohistochemical detection of p16 provides a new technology to study the genetic alterations in and stages of progression of large numbers of morphologically defined pancreatic duct lesions.
Hemi-alkoxides from reactions of trimethylaluminium with aldehydes or ketones (1970)

One mole of an aldehyde or ketone reacts stoicheiometrically with one mole of trimethylaluminium dimer at ambient temperature in hydrocarbon solvents to form a "hemi-alkoxide" in which an alkoxy group and a methyl group bridge two aluminium atoms. Benzaldehyde, acetone, acetophenone, benzophenone, and cyclohexanone give p-alkoxy-p-methyl-tetramethyldialuminiums (Me2A1.OR.Me.AlMe2; R = CHPhMe, But, CphMea2 CPh2Me, and 1-methylcyclohexyl, respectively). Such compounds can also be prepared in low yields starting from alcohols, rather than aldehydes or ketones. The analogous compound, NezAl.N=CPhMe.Me.AlMez, is formed by reaction of benzonitrile with one mole of trimethylaluminium dimer in the absence of solvent at 100º. Exchange of methyl groups between bridge and terminal sites in these new compounds has been studied by proton magnetic resonance spectroscopy.
Incidence and prevalence of neurological disorders associated with HIV since the introduction of highly active antiretroviral therapy (HAART) (2000)

The prevalence of the most frequent HIV associated neurological disorders and incidence of toxoplasma encephalitis decreased since the introduction of HAART. This may be due to the improvement of immunostatus by HAART as demonstrated by the higher CD4+ cell count in the later time interval. Direct antiretroviral effects within the nervous system may be considered causative as well. The prevalence and incidence of HIV associated neurological disorders and opportunistic CNS infections decreased after introduction of HAART.
Coexistence of different pathways in the metabolism of n-propylbenzene by Pseudomonas sp (1979)

Pseudomonas desmolytica S449B1 and Pseudomonas convexa S107B1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. The same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. A ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. Together with the results of oxygen uptake experiments, the data obtained suggested not only the existence of a reductive step to form 2-hydroxy-6-oxononanoic acid, but also the coexistence of two different pathways in the metabolism of n-propylbenzene by the strains used.