gebhart/patrepeval huggingface dataset:
Differential in vitro activities of ionophore compounds against Plasmodium falciparum and mammalian cells (1996)

Twenty-two ionophore compounds were screened for their antimalarial activities. They consisted of true ionophores (mobile carriers) and channel-forming quasi-ionophores with different ionic specificities. Eleven of the compounds were found to be extremely efficient inhibitors of Plasmodium falciparum growth in vitro, with 50% inhibitory concentrations of less than 10 ng/ml. Gramicidin D was the most active compound tested, with 50% inhibitory concentration of 0.035 ng/ml. Compounds with identical ionic specificities generally had similar levels of antimalarial activity, and ionophores specific to monovalent cations were the most active. Compounds were further tested to determine their in vitro toxicities against mammalian lymphoblast and macrophage cell lines. Nine of the 22 compounds, i.e., alborixin, lonomycin, nigericin, narasin, monensin and its methylated derivative, lasalocid and its bromo derivative, and gramicidin D, most specific to monovalent cations, were at least 35-fold more active in vitro against P. falciparum than against the two other mammalian cell lines. The enhanced ability to penetrate the erythrocyte membrane after infection could be a factor that determines ionophore selectivity for infected erythrocytes.
The N‐terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide (2006)

Autotaxin, also known as ENPP2, was originally isolated from the culture medium of melanoma cells as a cell-motility promoting protein. It regulates cell growth, motility, and angiogenesis through the production of lysophosphatidic acid and sphingosine 1-phosphate. Because autotaxin shows overall structural similarity to the well-characterized PC-1, it has been assumed to be a type II transmembrane protein that is expressed on the cell surface and is released into the extracellular space after proteolytic cleavage. We found, however, that while autotaxin was efficiently secreted into the extracellular space both in vitro and in vivo, it was expressed neither on the surfaces of autotaxin-transfected cells nor on those of the autotaxin-expressing choroid plexus epithelium cells. N-terminal sequencing of the secreted autotaxin revealed that it was cleaved at two N-terminal sites that match the consensus sequences for cleavage by a signal peptidase and furin. In addition, when translated in vitro, autotaxin was co-translationally translocated into microsome membranes, and its N-terminal 3-kDa fragment corresponding to a signal sequence was cleaved. These data demonstrate that the N-terminal hydrophobic sequence of autotaxin functions as a signal peptide, not as a transmembrane segment, and thus autotaxin is synthesized as a secreted protein.
An Adenoviral Platform for Selective Self‐Assembly and Targeted Delivery of Nanoparticles (2008)

Metallic nanoparticles (NPs) can be used for the diagnosis, imaging, and therapy of tumors and cardiovascular disease. However, targeted delivery of NPs to specific cells remains a major limitation for clinical realization of these potential treatment options. Herein, a novel strategy for the specific coupling of NPs to a targeted adenoviral (Ad) platform to deliver NPs to specific cells is defined. Genetic manipulation of the gene-therapy vector is combined with a specific chemical coupling strategy. In particular, a high-affinity interaction between a sequence of six-histidine amino acid residues genetically incorporated into Ad capsid proteins and nickel(II) nitrilotriacetic acid on the surface of gold NPs is employed. The selective self-assembly of gold NPs and Ad vectors into multifunctional platforms does not negatively affect the targeting of Ad to specific cells. This opens the possibility of using Ad vectors for targeted NP delivery, thereby providing a new type of combinatorial approach for the treatment of diseases that involves both nanotechnology and gene therapy.
Evicted variables and the interaction of global register allocation and symbolic debugging (1993)

A symbolic debugger allows a user to display the values of program variables at a breakpoint. However, problems arise if the program is translated by an optimizing compiler. This paper addresses the effects of global register allocation and assignment: a register assigned to a variable V may not be holding V's value at a breakpoint since the register can also be assigned to other variables. We define the problem of determining whether a variable is in its assigned register as the residence problem. Prior work on debugging of optimized code has focused on the currency problem; detecting whether a variable's run-time value is the expected value. Determining residence is a more serious problem than currency detection. We present a data flow algorithm that accurately computes a variable's residency, by determining when a variable becomes evicted from its register. We measure the effectiveness of different approaches to determine variable residence for three C programs from the SPEC suite.
Empirical Research of the Impact of Capital Structure on Agency Cost of Chinese Listed Companies (2013)

This paper, selecting 775 listed companies from Shanghai and Shenzhen stock markets during three years from 2010 to 2012 as samples, studies the relationship between agency cost and capital structure, using two econometrics methods which are ordinary least squares (OLS) and panel data respectively. Capital structure is calculated by debt-to-asset ratio and long-term liability rate while agency cost is measured by overhead expenses rate and asset turnover rate. The result shows agency cost has a slightly negative correlation to debt-to-asset ratio and there is a positive and insignificant correlation relationship between long-term liability rate and agency cost.
Support Vector Machines for Transient Stability Analysis of Large-Scale Power Systems (2004)

The pattern recognition approach to transient stability analysis (TSA) has been presented as a promising tool for online application. This paper applies a recently introduced learning-based nonlinear classifier, the support vector machine (SVM), showing its suitability for TSA. It can be seen as a different approach to cope with the problem of high dimensionality. The high dimensionality of power systems has led to the development and implementation of feature selection techniques to make the application feasible in practice. SVMs' theoretical motivation is conceptually explained and they are tested with a 2684-bus Brazilian system. Aspects of model adequacy, training time, classification accuracy, and dimensionality reduction are discussed and compared to stability classifications provided by multilayer perceptrons.
Autonomous Vehicle Technology: A Guide for Policymakers (2016)

Self-driving vehicles offer the promise of significant benefits to society, but raise several policy challenges, including the need to update insurance liability regulations and privacy concerns such as who will control the data generated by this technology.
Depth Video Coding Using Adaptive Geometry Based Intra Prediction for 3-D Video Systems (2011)

Depth video coding is an essential part of 3-D video processing systems. Specifically, object boundary regions are important in depth video coding since these regions significantly affect the visual quality of a synthesized view. In this paper, we propose an efficient depth video coding method to determine precise intra prediction modes and thereby reduce the loss of boundary information. To achieve this objective, we analyze and exploit statistical and geometric characteristics of the depth video. Experimental results subsequently show that the proposed method performs better than the original intra prediction of H.264/AVC in terms of bit savings and rendering quality.
High-speed low-power silicon MESFET parallel multipliers (1982)

The design of fast low-power silicon LSI MESFET parallel multipliers is studied. The architecture of the multipliers and the designs of the functional blocks are discussed. The overall performance of the multipliers is estimated from the simulated performances of the functional blocks and from system simulations with a logic simulator. The actual performance of 8/spl times/8 and 10/spl times/10 bit TTL-compatible multipliers, fabricated with a 2.5 /spl mu/m silicon MESFET technology (1.5-2 /spl mu/m effective dimensions) is compared to the simulations.
Catalytic etching of platinum foils and thin films in hydrogen-oxygen mixtures (1988)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCatalytic etching of platinum foils and thin films in hydrogen-oxygen mixturesVictor W. Dean, Michael Frenklach, and Jonathan PhillipsCite this: J. Phys. Chem. 1988, 92, 20, 5731–5738Publication Date (Print):October 1, 1988Publication History Published online1 May 2002Published inissue 1 October 1988https://pubs.acs.org/doi/10.1021/j100331a037https://doi.org/10.1021/j100331a037research-articleACS PublicationsRequest reuse permissionsArticle Views114Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
CCD structures implemented in standard 0.18 μm CMOS technology (2008)

A novel concept of implementing charge-coupled devices (CCDs) in a 0.18 µm CMOS technology is developed. This structure is targeted towards large pixels for medical applications. No major process modifications are necessary for implementing this structure.
Channel protein engineering: synthetic 22-mer peptide from the primary structure of the voltage-sensitive sodium channel forms ionic channels in lipid bilayers. (1988)

A synthetic 22-mer peptide that mimics the sequence of a putative pore segment of the voltage-dependent sodium channel forms transmembrane ionic channels in lipid bilayers. Several features of the authentic sodium channel are exhibited by the synthetic peptide: (i) The single channel conductance of the most frequent event is 20 pS in 0.5 M NaCl. (ii) The single channel open and closed lifetimes are in the ms time range. (iii) The synthetic channel discriminates cations over anions but is nonselective between Na+ and K+. However, the synthetic channel displays no significant voltage dependence. Energetic considerations suggest a bundle of four parallel amphipathic alpha-helices as the most plausible channel structure. The synthetic 22-mer channel-forming peptide allows study of the mechanisms of ion permeation through sodium channels by protein engineering techniques.
Brain and heart sodium channel subtype mRNA expression in rat cerebral cortex. (1991)

The expression of mRNAs coding for the alpha subunit of rat brain and rat heart sodium channels has been studied in adult and neonatal rat cerebral cortex using the reverse transcription-polymerase chain reaction (RT-PCR). Rat brain sodium channel subtype I, II, IIA, and III sequences were simultaneously amplified in the same PCR using a single oligonucleotide primer pair matched to all four subtype sequences. Identification of each subtype-specific product was inferred from the appearance of unique fragments when the product was digested with specific restriction enzymes. By using this RT-PCR method, products arising from mRNAs for all four brain sodium channel subtypes were identified in RNA extracted from adult rat cerebral cortex. The predominant component was type IIA with lesser levels of types I, II, and III. In contrast, the type II and IIA sequences were the predominant RT-PCR products in neonatal rat cortex, with slightly lower levels of type III and undetectable levels of type I. Thus, from neonate to adult, type II mRNA levels decrease relative to type IIA levels. Using a similar approach, we detected mRNA coding for the rat heart sodium channel in neonatal and adult rat cerebral cortex and in adult rat heart. These results reveal that mRNAs coding for the heart sodium channel and all four previously sequenced rat brain sodium channel subtypes are expressed in cerebral cortex and that type II and IIA channels may be differentially regulated during development.
Thermal Conductivity of poly-<i>p</i>-phenylene-2,6-benzobisoxazole Film along the In-Plane Axis in the 10–300 K Temperature Range (2018)

The thermal conductivities of compression molded thin films of poly-p-phenylene-2,6-benzobisoxazole (PBO) were measured in directions along an in-plane axis in the 10–300 K temperature range by a steady-state heat flow method, with interest in the use of the material for superconductivity applications. The thermal conductivities of the PBO films increased from 0.3 W/mK to 9.0 W/mK with increasing temperature from 10 K to 300 K and these were much higher than those of polyimide films, epoxy resin and glass fiber reinforced plastics at all temperatures. The 9.0 W/mK at 300 K was 60% of that of stainless steel (SUS304). It was 6 W/mK at 150 K, which was half that of SUS304 and was 3.3 W/mK at 77 K, which was 33% of that of SUS304. The thermal conductivities of the PBO films were lower than those of a cloth of high strength ultrahigh molecular weight polyethylene fiber reinforced plastics in the 30 K–180 K temperature range and were almost equivalent to its values in the 180 K–300 K temperature range. The main contribution to the thermal conduction in the PBO films was from thermal phonon conduction along the molecular chains. Although many kinds of high thermal conductivity polymeric materials have been prepared by a uni-directional drawing process or by adding high thermal conductive additives, the PBO film showed high thermal conductivity without a uni-directional drawing process or high thermal conductive additive.
Combined CO<sub>2</sub>-philicity and Ordered Mesoporosity for Highly Selective CO<sub>2</sub> Capture at High Temperatures (2015)

Various dry sorbents have been lately introduced as promising media to capture carbon dioxide (CO2). However, it is still desirable to further improve their performance in diverse aspects, and high temperature selectivity of CO2 over other gases is clearly one of them. Here, we report a co-assembly approach to turn nonporous melamine resin to a highly ordered mesoporous polymeric network (space group: Im3̅m) containing high nitrogen content (∼18 at%). This mesoporous network shows anomalously increasing CO2/N2 selectivity with temperature rise, with the selectivity at 323 K reaching 117 (Henry method). This selectivity behavior is attributed to a combined effect of the high nitrogen content allowing for high binding affinity with CO2 and well-defined mesopores (2.5-2.9 nm) accelerating release of N2 with temperature rise. The given orthogonal approach suggests a new direction in designing dry sorbents with excellent selectivities at high temperatures.
Comparison of screening methods for high-throughput determination of oil yields in micro-algal biofuel strains (2012)

The phenotypic and phylogenetic diversity of micro-algae capable of accumulating triacylglycerols provides a challenge for the accurate determination of biotechnological potential. High-yielding strains are needed to improve economic viability and their compositional information is required for optimizing biodiesel properties. To facilitate a high-throughput screening programme, a very rapid direct-derivatization procedure capable of extracting lyophilized material for GC analysis was compared with a scaled-down Folch-based method. This was carried out on ten micro-algal strains from 6 phyla where the more rapid direct-derivatization approach was found to provide a more reliable measure of yield. The modified Folch-based procedure was found to substantially underestimate oil yield in one <i>Chlorella</i> species (<i>P</i> < 0.01). In terms of fatty acid composition however, the Folch procedure proved to be slightly better in recovering polyunsaturated fatty acids, in six out of the ten strains. Therefore, direct-derivatization is recommended for rapid determination of yields in screening approaches but can provide slightly less compositional accuracy than solvent-based extraction methods.
Visual Simulation of Heat Shimmering and Mirage (2006)

We provide a physically-based framework for simulating the natural phenomena related to heat interaction between objects and the surrounding air. We introduce a heat transfer model between the heat source objects and the ambient flow environment, which includes conduction, convection, and radiation. The heat distribution of the objects is represented by a novel temperature texture. We simulate the thermal flow dynamics that models the air flow interacting with the heat by a hybrid thermal lattice Boltzmann model (HTLBM). The computational approach couples a multiple-relaxation-time LBM (MRTLBM) with a finite difference discretization of a standard advection-diffusion equation for temperature. In heat shimmering and mirage, the changes in the index of refraction of the surrounding air are attributed to temperature variation. A nonlinear ray tracing method is used for rendering. Interactive performance is achieved by accelerating the computation of both the MRTLBM and the heat transfer, as well as the rendering on contemporary graphics hardware (GPU).
Topical Treatment of Onychomycosis with Amorolfine 5% Nail Lacquer: Comparative Efficacy and Tolerability of Once and Twice Weekly Use (1992)

456 patients with onychomycosis were treated once or twice weekly for up to 6 months with amorolfine 5% nail lacquer in an open, randomized study. The patients were examined at monthly intervals during treatment and followed-up 1 and 3 months after completion of treatment. Slightly better cure rates were achieved with twice weekly use than with once weekly use (overall cure rates 54.2 vs. 46.0%, p = 0.4). An overall cure or improvement was achieved in 74 and 68% of patients receiving twice- and once-weekly treatment, respectively. The mycological cure rate was 76.1% for twice-weekly treatment and 70.6% for once-weekly treatment. The nail lacquer was extremely well tolerated; 4 out of 456 patients reported mild local irritation. Plasma levels of amorolfine were determined in 19 patients and found to be below the detection limit of 0.5 ng/ml in all cases.
Analysis of a <i>Clostridium josui</i> Cellulase Gene Cluster Containing the <i>man5A</i> Gene and Characterization of Recombinant Man5A (2010)

A cellulase gene cluster of Clostridium josui was sequenced, and was found to encode 11 proteins responsible for cellulosome (cellulolytic complex) formation, viz., cipA, cel48A, cel8A, cel9A, cel9B, orfX, cel9C, cel9D, man5A, cel9E, and cel5B, in order from the upstream side. All the predicted enzymes had a dockerin module, suggesting that these proteins are members of the C. josui cellulosome. Among these genes, the man5A gene encoding β-mannanase was expressed in Escherichia coli and the recombinant enzyme (rMan5A) was characterized. rMan5A showed strong activity toward carob galactomannan and low activity toward guar gum, suggesting that it prefers non-galactosylated mannan to galactomannan. This enzyme hydrolyzed ivory nut mannan to produce mainly mannotriose and larger mannooligosaccharides, and was not active toward mannotriose. An antiserum raised against the recombinant enzyme detected Man5A in the culture supernatants of C. josui, which was grown on either ball-milled cellulose or glucose as a carbon source.
Interactions and Oligomerization of Hantavirus Glycoproteins (2009)

In this report the basis for the structural architecture of the envelope of hantaviruses, family Bunyaviridae, is systematically studied by the interactions of two glycoproteins N and C (Gn and Gc, respectively) and their respective disulfide bridge-mediated homo- and heteromeric oligomerizations. In virion extracts Gn and Gc associated in both homo- and hetero-oligomers which were, at least partially, thiol bridge mediated. Due to strong homo-oligomerization, the hetero-oligomers of Gn and Gc are likely to be mediated by homo-oligomeric subunits. A reversible pH-induced disappearance of a neutralizing epitope in Gc and dissociation of the Gn-Gc complex at pH values below 6.2 provide proteochemical evidence for the fusogenicity of Gc. Incomplete inactivation of virions at acidic pH indicates that additional factors are required for hantavirus fusion, as in the case of pestiviruses of the Flaviviridae. Based on similarities to class II fusion proteins, a structure model was created of hantavirus Gc using the Semliki Forest virus E1 protein as a template. In total, 10 binding regions for Gn were found by peptide scanning, of which five represent homotypic (Gn(I) to Gn(V)) and five represent heterotypic (Gc(I) to Gc(V)) interaction sites that we assign as intra- and interspike connections, respectively. In conclusion, the glycoprotein associations were compiled to a model wherein the surface of hantaviruses is formed of homotetrameric Gn complexes interconnected with Gc homodimers. This organization would create the grid-like surface pattern described earlier for hantaviruses in negatively stained electron microscopy specimens.
Dye-less color filter fabricated by roll-to-roll imprinting for liquid crystal display applications (2009)

A diffractive grating is promising for color separation to effectively replace conventional absorptive dye color filter in liquid crystal displays. In this paper, we demonstrated a color separation module consisting of an aspheric-lenticular lens array and a blazed grating to substitute for the dye color filter. Each component was designed to match the recent fabrication ability of our roll-to-roll imprinting. The measurement results of a prototype module showed a gain factor of transmission efficiency three times more than that of conventional color filters.
Hypoxia-inducible factor–1 and associated upstream and downstream proteins in the pathophysiology and management of glioblastoma (2014)

Glioblastoma multiforme (GBM) is a highly aggressive brain tumor with an exceptionally poor patient outcome despite aggressive therapy including surgery, radiation, and chemotherapy. This aggressive phenotype may be associated with intratumoral hypoxia, which probably plays a key role in GBM tumor growth, development, and angiogenesis. A key regulator of cellular response to hypoxia is the protein hypoxia-inducible factor–1 (HIF-1). An examination of upstream hypoxic and nonhypoxic regulation of HIF-1 as well as a review of the downstream HIF-1– regulated proteins may provide further insight into the role of this transcription factor in GBM pathophysiology. Recent insights into upstream regulators that intimately interact with HIF-1 could provide potential therapeutic targets for treatment of this tumor. The same is potentially true for HIF-1–mediated pathways of glycolysis-, angiogenesis-, and invasion-promoting proteins. Thus, an understanding of the relationship between HIF-1, its upstream protein regulators, and its downstream transcribed genes in GBM pathogenesis could provide future treatment options for the care of patients with these tumors.