gebhart/patrepeval huggingface dataset:
Antimicrobial Activity of Intrinsic Antimicrobial Polymers Based on Poly((tertbutyl-amino)-methyl-styrene) Against Selected Pathogenic and Spoilage Microorganisms Relevant in Meat Processing Facilities (2017)

Objective: Antimicrobial materials are used as a possible approach to improve hygienic conditions in the food industry. The aim of this study was the investigation of the antimicrobial activity of the homopolymer of poly((tertbutyl- amino)-methyl-styrene) (poly(TBAMS)) and of the copolymer poly(TBAMS:acrylonitrile) [1:1] against microorganism present on meat processing equipment. Method: Antimicrobial polymers were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry analysis. The antimicrobial activity against various pathogenic and spoilage bacteria (S. aureus, E. coli, L. monocytogenes, Salmonella spp., Pseudomonas spp., B. thermosphacta) was determined using a modified test method based on the Japanese Industrial Standard JIS Z 2801: 2000. Furthermore, the influence of high initial bacterial counts (up to 8.9 log10 cfu/ml) as well as the exposure of bacteria in mixed cultures on the antimicrobial activity was evaluated. Results: Spectroscopy identified the homopolymer poly(TBAMS) as well as a successful copolymerization with acrylonitrile. Results of antimicrobials tests showed significant reductions of bacterial counts on both polymers compared with the reference material of microorganisms in pure culture after 2 hour at 35A‚Â°C. L. monocytogenes, E. coli and S. aureus were reduced to the detection limit (>4.2 log10-units). P. fluorescens was less sensitive to poly(TBAMS)-based films, especially to the copolymer. The homopolymer offers slightly higher activity than the copolymer, but glass transition temperature was lower. Tests with mixed cultures affirmed the dependency of activity on bacteria species. A tendency of higher antimicrobial activity against gram-positive was observed, if high initial counts were used; however, significant reduction of gram-negative were still determined. Conclusion: Poly(TBAMS)-films show excellent antimicrobial properties against microorganisms relevant in meat processing facilities, and the implementation of those surfaces could contribute to improving the hygienic conditions during production and processing.
Novel donors of nitric oxide derived of S-nitrosocysteine possessing antioxidant activities (1999)

Novel S-nitrosothiols possessing a phenolic function were investigated as nitric oxide (NO) donors. A study of NO release from these derivatives was carried out by electron spin resonance (ESR). All compounds gave rise to a characteristic three-line ESR signal in the presence of the complex [Fe(II)(MGD)2], revealing the formation of the complex [Fe(II)(MGD)2(NO)]. Furthermore, tests based on cytochrome c reduction were performed in order to study the ability of each phenolic disulfide, the final organic decomposition product of S-nitrosothiols, to trap superoxide radical anion (O2-). This study revealed a high reactivity of 1b and 3b towards O2-. For these two compounds, the respective inhibitory concentration (IC) 50 values were 92 microM and 43 microM.
Ethyl esters from the single‐phase base‐catalyzed ethanolysis of vegetable oils (2003)

Abstract The effects of alcohol/oil molar ratio, base concentration, and temperature on the single‐phase base‐catalyzed ethanolyses of sunflower and canola oils were determined. The use of tetrahydrofuran as co‐solvent, as well as higher than usual alcohol/substrate molar ratios, prevented glycerol separation. This allowed each reaction to reach equilibrium rather than just steady‐state conditions. High conversions of oil lowered the concentrations of MG and DG surfactants in the products, and thereby mitigated the formation of emulsions usually associated with ethanolysis reactions. An alcohol/oil molar ratio of 25∶1, together with the necessary amount of cosolvent, gave optimal results. At this molar ratio, despite equilibrium being achieved, ethanolysis, unlike methanolysis, did not quite produce biodiesel‐standard material, the MG content being approximately 1.5 mass%. For methanolysis and 1‐butanolysis, the corresponding values were 0.6 and 2.0 mass%, respectively. The use of 1.4 mass% KOH (equivalent to 1.0 mass% NaOH) led to ethanolysis equilibrium within 6–7 min at 23°C rather than 15 min when only 1.0 mass% was used. At 60°C, equilibrium was reached within only 2 min. Soybean and canola oils behaved the same.
Sequence requirements for incorporation of human immunodeficiency virus Gag-?-galactosidase fusion proteins into virus-like particles (1999)

The incorporation of human immunodeficiency virus type 1 (HIV-1) Gag-beta-galactosidase (Gag-beta-gal; GBG) fusion proteins into HIV virus-like particles in the presence of HIV Gag proteins was studied. HIV Gag-beta-gal fusion constructs were cotransfected individually into COS7 cells with or without an HIV Gag protein expression plasmid. Release of HIV GBG fusion proteins from the cells were measured by assay of the medium versus intracellular beta-gal activities. Analysis indicates that fusion proteins (constructs HIVGBG, GBG 1919 and 1877) retaining the C-terminal portion of the CA and the adjacent NC domains were efficiently assembled into virus-like particles. Fusion proteins with deleted sequences covering the N-terminal portions of the gag sequences (GBG 831, 1147, 1419, 1447, 1511, 1552, 1600, 1630, 1684, 1715, and 1752) were impaired in entry into virus-like particles. The presence of CA major homology region (MHR) in the fusion proteins had no significant effects on inducing fusion protein incorporation when the C-terminal CA sequences in the fusion proteins were truncated (GBG 1841 and 1801). Subcellular fractionation studies indicated that most fusion proteins including the nonmyristylated one were enriched in the crude membrane fraction. Exceptions to this rule were fusion proteins with intact MHR but truncated C-terminal CA sequences, which possessed low levels of membrane association. However, assembly of fusion proteins into HIV Gag particles did not correlate with their subcellular fractionation or immunofluorescence localization patterns. Overall, the studies suggest that the very C-terminal CA and adjacent NC sequences are the primary determinants for incorporation of HIV Gag-beta-gal fusion proteins into virus particles.
Lipoid pH indicators as probes of electrical potential and polarity in micelles (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLipoid pH indicators as probes of electrical potential and polarity in micellesMarta S. Fernandez and Peter FromherzCite this: J. Phys. Chem. 1977, 81, 18, 1755–1761Publication Date (Print):September 1, 1977Publication History Published online1 May 2002Published inissue 1 September 1977https://pubs.acs.org/doi/10.1021/j100533a009https://doi.org/10.1021/j100533a009research-articleACS PublicationsRequest reuse permissionsArticle Views1291Altmetric-Citations536LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Historical and projected coastal Louisiana land changes: 1978-2050 (2003)

An important component of the Louisiana Coastal Area (LCA) Comprehensive Coastwide Ecosystem Restoration Study is the projection of a “future condition” for the Louisiana coast if no further restoration measures were adopted. Such a projection gives an idea of what the future might hold without implementation of the LCA plan and provides a reference against which various ecosystem restoration proposals can be assessed as part of the planning process. One of the most fundamental measures of ecosystem degradation in coastal Louisiana has been the conversion of land (mostly emergent vegetated habitat) to open water. Thus, the projection of the future condition of the ecosystem must be based upon the determination of future patterns of land and water. To conduct these projections, a multidisciplinary LCA Land Change Study Group was formed that included individuals from agencies and academia with expertise in remote sensing, geographic information systems (GIS), ecosystem processes, and coastal land loss. Methods were based upon those used in prior studies for Coast 2050 (Louisiana Coastal Wetlands Conservation and Restoration Task Force [LCWCRTF] and the Wetlands Conservation and Restoration Authority 1998, 1999) and modified as described here to incorporate an improved understanding of coastal land loss and land gain processes with more advanced technical capabilities. The basic approach is to use historical data to assess recent trends in land loss and land gain and to project those changes into the future, taking into account spatial variations in the patterns and rates of land loss and land gain. This approach is accomplished by developing a base map, assessing and delineating areas of similar land change (polygons), and projecting changes into the future. This report describes the methodology and compares the current land change projection to previous projections.
A convolutional neural network based 3D ball tracking by detection in soccer videos (2019)

Tracking of ball in sports videos is one of the most challenging tasks in computer vision and video processing domain. Recent ball tracking approaches fail to handle tracking of a small size and fast moving ball. Inaccurate 2D ball detection leads to further deterioration of 3D ball tracking results. This paper presents a soccer ball tracking by detection approach using a pre-trained Convolutional Neural Network (CNN). The proposed algorithm used CNN for identifying ball from background and other moving objects like players and referees. The 2D ball detection results are fine-tuned for identifying true ball positions. True ball positions, from cameras shooting the scene from different angle, are further mapped on ground plane. The actual ball movement is tracked in 3D from top-view. Experiments show that the proposed algorithm can tackle challenges like small ball size, shape changes, occlusion and tracking high-speed balls.
Niraparib in ovarian cancer: results to date and clinical potential (2017)

Ovarian cancer is the first cause of death from gynaecological malignancy. Germline mutation in <i>BRCA1</i> and <i>2</i>, two genes involved in the mechanisms of reparation of DNA damage, are showed to be related with the incidence of breast and ovarian cancer, both sporadic and familiar. PARP is a family of enzymes involved in the base excision repair (BER) system. The introduction of inhibitors of PARP in patients with <i>BRCA</i>-mutated ovarian cancer is correlated with the concept of synthetic lethality. Among the PARP inhibitors introduced in clinical practice, niraparib showed interesting results in a phase III trial in the setting of maintenance treatment in ovarian cancer, after platinum-based chemotherapy. Interestingly, was niraparib showed to be efficacious not only in <i>BRCA</i>-mutated patients, but also in patients with other alterations of the homologous recombination (HR) system and in patients with unknown alterations. These results position niraparib as the first PARP-inhibitor with clinically and statistically significant results also in patients with no alterations in <i>BRCA 1/2</i> and other genes involved in the DNA repair system. Even if the results are potentially practice-changing, the action of niraparib must be further studied and deepened.
Fe-Co and Fe-V Alloys for Pressure Calibration in the 130- to 300-kbar Region (1967)

Fe-Co and Fe-V alloys, tested in static ultrahigh pressure apparatus, have been found to have resistance jumps resulting from the α to ε transformation, as in pure Fe. The pressure of initiation of the transformation increases with the alloy content; from 131 kbar for pure Fe up to 290 kbar for 20 wt% Co. The V alloys rise much faster: 280 kbar at 6 wt%. The Fe-Co alloys can serve as convenient samples to pressure-calibrate apparatus in the 160 to 300-kbar region. The difference between the pressures of initiation of transformation under static conditions, compared to shock conditions, is zero for pure Fe and progressively greater as the alloy content increases.
Acid hydrolases in leukocytes and platelets of normal subjects and in patients with Gaucher's and Fabry's disease. (1976)

Lymphocytes, monocytes, neutrophilic granulocytes and platelets were each separated to greater than 95% purity from six normal subjects, three patients with Gaucher's disease, two heterozygotes for Gaucher's disease, and one patient with Fabry's disease. Activities of the following acid hydrolases were determined: "acid" (pH 4.0) beta-glucosidase, pH 5.0 beta-glucosidase, alpha-galactosidase, alpha-arabinosidase, alpha-mannosidase, alpha-glucosidase, beta-glucuronidase, beta-galactosidase, beta-hexosaminidase, and acid phosphatase. Enzymatic activity varied greatly with cell type and the enzyme being measured; the importance of assaying pure preparations especially for heterozygote detection is emphasized. Gaucher's disease patients' cells were found to be deficient in the pH 4.0 acid beta-glucosidase, variable in the pH 5.0 beta-glucosidase, and normal in all other acid hydrolases tested, including acid phosphatase, the activity of which is known to be elevated in plasma. Blood cells of a patient with Fabry's disease were deficient in alpha-galactosidase and normal in all other acid hydrolases tested.
Subdiffusive motion of bacteriophage in mucosal surfaces increases the frequency of bacterial encounters (2015)

Bacteriophages (phages) defend mucosal surfaces against bacterial infections. However, their complex interactions with their bacterial hosts and with the mucus-covered epithelium remain mostly unexplored. Our previous work demonstrated that T4 phage with Hoc proteins exposed on their capsid adhered to mucin glycoproteins and protected mucus-producing tissue culture cells in vitro. On this basis, we proposed our bacteriophage adherence to mucus (BAM) model of immunity. Here, to test this model, we developed a microfluidic device (chip) that emulates a mucosal surface experiencing constant fluid flow and mucin secretion dynamics. Using mucus-producing human cells and Escherichia coli in the chip, we observed similar accumulation and persistence of mucus-adherent T4 phage and nonadherent T4∆hoc phage in the mucus. Nevertheless, T4 phage reduced bacterial colonization of the epithelium >4,000-fold compared with T4∆hoc phage. This suggests that phage adherence to mucus increases encounters with bacterial hosts by some other mechanism. Phages are traditionally thought to be completely dependent on normal diffusion, driven by random Brownian motion, for host contact. We demonstrated that T4 phage particles displayed subdiffusive motion in mucus, whereas T4∆hoc particles displayed normal diffusion. Experiments and modeling indicate that subdiffusive motion increases phage-host encounters when bacterial concentration is low. By concentrating phages in an optimal mucus zone, subdiffusion increases their host encounters and antimicrobial action. Our revised BAM model proposes that the fundamental mechanism of mucosal immunity is subdiffusion resulting from adherence to mucus. These findings suggest intriguing possibilities for engineering phages to manipulate and personalize the mucosal microbiome.
Delaware Valley Schweitzer Fellows Program Seeking Sponsors (2006)

academic medical center, Jefferson has been a leader in healthcare issues involving public policy and reducing disparities in health and healthcare, which is
Implementation of atomic primitives on distributed shared memory multiprocessors (2002)

In this paper we consider several hardware implementations of the general-purpose atomic primitives fetch and /spl Phi/, compare and swap, load linked, and store conditional on large-scale shared-memory multiprocessors. These primitives have proven popular on small-scale bets-based machines, but have yet to become widely available on large-scale, distributed shared memory machines. We propose several alternative hardware implementations of these primitives, and then analyze the performance of these implementations for various data sharing patterns. Our results indicate that good overall performance can be obtained by implementing compare and swap in the cache controllers, and by providing an additional instruction to load an exclusive copy of a cache line.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Role of the Catalytic Base in the Protein Tyrosine Kinase Csk (1995)

A potential distinguishing feature between protein tyrosine kinases and homologous serine/threonine kinases is the function of the catalytic base in these enzymes. In this study, we show that a peptide containing the unnatural amino acid trifluorotyrosine shows remarkably similar efficiency as a substrate of the tyrosine kinase Csk (C-terminal Src kinase) compared with the corresponding tyrosine-containing peptide despite a 4-unit change in the phenolic pKa. These results argue against the importance of early tyrosine deprotonation by a catalytic base in Csk. To further explore the role of the proposed catalytic base, the Csk mutant protein D314E was produced. This mutant displayed a significant reduction in kcat (approximately 10(4)) but relatively little effect on substrate Km values compared with wild-type Csk. Examination of the thio effect (kcat-ATP/kcat-adenosine 5'-O-(thiotriphosphate)) for D314E Csk led to the suggestion that a role of aspartate 314 may be to enhance the reactivity of the gamma-phosphate of ATP toward electrophilic attack. These results may have significant impact on protein tyrosine kinase inhibitor design.
Audiovisual SlowFast Networks for Video Recognition (2020)

We present Audiovisual SlowFast Networks, an architecture for integrated audiovisual perception. AVSlowFast has Slow and Fast visual pathways that are deeply integrated with a Faster Audio pathway to model vision and sound in a unified representation. We fuse audio and visual features at multiple layers, enabling audio to contribute to the formation of hierarchical audiovisual concepts. To overcome training difficulties that arise from different learning dynamics for audio and visual modalities, we introduce DropPathway, which randomly drops the Audio pathway during training as an effective regularization technique. Inspired by prior studies in neuroscience, we perform hierarchical audiovisual synchronization to learn joint audiovisual features. We report state-of-the-art results on six video action classification and detection datasets, perform detailed ablation studies, and show the generalization of AVSlowFast to learn self-supervised audiovisual features. Code will be made available at: https://github.com/facebookresearch/SlowFast.
The sirtuins, oxidative stress and aging: an emerging link (2013)

Reactive oxygen species (ROS) are a family of compounds that can oxidatively damage cellular macromolecules and may influence lifespan. Sirtuins are a conserved family of nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylases that regulate lifespan in many model organisms including yeast and mice. Recent work suggests that sirtuins can modulate ROS levels notably during a dietary regimen known as calorie restriction which enhances lifespan for several organisms. Although both sirtuins and ROS have been implicated in the aging process, their precise roles remain unknown. In this review, we summarize current thinking about the oxidative stress theory of aging, discuss some of the compelling data linking the sirtuins to ROS and aging, and propose a conceptual model placing the sirtuins into an ROS-driven mitochondria-mediated hormetic response.
Qualifying composition dependent <i>p</i> and <i>n</i> self-doping in CH3NH3PbI3 (2014)

We report the observation of self-doping in perovskite. CH3NH3PbI3 was found to be either n- or p-doped by changing the ratio of methylammonium halide (MAI) and lead iodine (PbI2) which are the two precursors for perovskite formation. MAI-rich and PbI2-rich perovskite films are p and n self-doped, respectively. Thermal annealing can convert the p-type perovskite to n-type by removing MAI. The carrier concentration varied as much as six orders of magnitude. A clear correlation between doping level and device performance was also observed.
Hydrolysis of toxic organophosphorus compounds by o-iodosobenzoic acid and its derivatives (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHydrolysis of toxic organophosphorus compounds by o-iodosobenzoic acid and its derivativesPhilip S. Hammond, Jeffry S. Forster, Claire N. Lieske, and H. Dupont DurstCite this: J. Am. Chem. Soc. 1989, 111, 20, 7860–7866Publication Date (Print):September 1, 1989Publication History Published online1 May 2002Published inissue 1 September 1989https://pubs.acs.org/doi/10.1021/ja00202a029https://doi.org/10.1021/ja00202a029research-articleACS PublicationsRequest reuse permissionsArticle Views286Altmetric-Citations56LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A System to Generate Test Data and Symbolically Programs ; CU-CS-060-75 (1975)

This paper describes a system that is currently being implemented to generate test data for programs written in ANSI FORTRAN. Given a path, the system symbolically executes the program and creates a set of constraints on the programâs input variables. If the set of constrains is linear, linear programming techniques are employed to solve the system of inequalities. A solution to the system of constrains is then a set of test data that will drive execution down the given path. If it can be determined that the set of constraints is inconsistent then the given path is shown to be nonexecutable. To increase the chance of detecting some of the more common programming errors, artificial constraints are temporarily created that simulate the error condition and an attempt is made to solve the augmented set of constraints. A symbolic representation of the programâs output variables in terms of the programâs input variables is also created. The symbolic representation is in a human readable form that facilitates error detection as well as being a possible aid in allegation generation and automatic program documentation..