gebhart/patrepeval huggingface dataset:
Excitons and the Absorption Edge in ZnSe (1967)

The optical absorption threshold at about 2.7 eV, near the lowest-energy fundamental edge, was investigated for cubic ZnSe single crystals. Absorption spectra are reported for temperatures between 2 and 200\ifmmode^\circ\else\textdegree\fi{}K, and absorption coefficients between 4 and 600 ${\mathrm{cm}}^{\ensuremath{-}1}$. Although contributions associated with defects dominated the results in most crystals, intrinsic absorption could be observed for $T\ensuremath{\gtrsim}60$\ifmmode^\circ\else\textdegree\fi{}K in the purest available crystals. This intrinsic absorption results from longitudinal-optical-phonon-assisted creation of excitons, as is shown by the good agreement between the observed magnitude, the temperature, and photon-energy dependence, and the absorption calculated for this mechanism. The relevant exciton states involve electrons and holes from band extrema near the center of the Brillouin zone; when created without phonon assistance, excitons from these same extrema also give the relatively very intense "direct transition" absorption lines. No evidence is found that any band gap is smaller than that at the zone center.
Generation of uniform lines with variable size using a beam shaping hybrid zoom optical system (2003)

A state-of-the-art laser beam shaper zoom optical system has been developed. This optical device, efficiently converts a 532 nm single-mode Gaussian intensity profile into top hat lines of variable size with peak-to-valley non-uniformities of ≤±8%. This hybrid zoom system is capable of generating line lengths ranging between 0.16 mm to 1 mm, and line widths of up to 15 μm. The lines are of square-like shape in the long axis while they remain Gaussian in the short axis. The system was designed, fabricated, and tested using a laser with a nominal input Gaussian beam waist of 2.25 mm at the 1/e<sup>2</sup> intensity points, and a nominal M<sup>2</sup> of less than 1.1. Techniques associated with the system alignment during the optical assembly and system integration are discussed. The optical design and test results of the diffractive-optical system are also presented.
Proton and carbon‐13 NMR studies of 2‐aminothiazoles and 2‐iminothiazolines (1987)

Abstract Proton and 13 C NMR chemical shifts and coupling constants of 2‐aminothiazoles and 2‐iminothiazolines are reported. The one‐bond CH, vicinal H‐H, geminal CH and vicinal CH coupling constants of 2‐aminothiazoles and 2‐iminothiazolines are discussed in terms of tautomerism between amino forms and imino forms. The site of charge centered either on the ring nitrogen or the exocyclic nitrogen atom in the protonated 2‐aminothiazole is also discussed.
Evidence for nitrate reductase expression during initiation of lateral roots by NAA in chicory (1998)

The supply of α-naphthaleneacetic acid (NAA), to excised chicory roots induced the formation of lateral root meristems mainly localized proximal to the pre-existing apical root meristem, in a region which does not initiate any lateral roots in control conditions. Inhibition of root elongation and concomitant enlargement of the apices were also observed. Quantification of IAA and cytokinin levels showed that the most reproducible and significant changes occurring after the NAA treatment consisted of a decrease in the level of zeatin-O-glucoside conjugates. Hydrolysis of these conjugates might deliver free zeatin-type compounds which were consumed during the lateral root growth. After 5 d, control excised roots contained a high level of amino acids, mainly as asparagine and arginine, probably issued from proteolysis associated to a senescent-like process. Conversely, in the presence of NAA, neither accumulation of amino acids nor a decrease of the total protein content of the tissue could be detected. Newly initiated meristems expressed the nia gene which encodes nitrate reductase, the first enzyme of the nitrate assimilatory pathway. Thus the increased expression of nitrate reductase which was observed in excised roots of chicory supplied with NAA (Vuylsteker et al., 1997b) may be ascribed to lateral root formation and development. The reinduction of nitrate reduction activity was driven by the increased demand for reduced nitrogen. Thus, the nia gene is one of the genes expressed during the early stages of root meristem formation.
Performance of personal access communications system-unlicensed B (1996)

The FCC has allocated the band between 1920 MHz and 1930 MHz for unlicensed personal communications services (UPCS) using isochronous or circuit operation. The UPCS spectrum is between the licensed PCS spectrum bands of 1850-1910 MHz and 1930-1990 MHz. Terminal interoperability in both the UPCS spectrum on private indoor wireless systems and the licensed spectrum on public PCS systems is desirable and encouraged by the FCC. This paper presents a port channel assignment process for the personal access communications system-unlicensed B (PACS-UB) which abides by the FCC etiquette for UPCS and discusses the corresponding uplink and downlink performance. Uplink power control is employed to improve the uplink performance. PACS-UB has a high degree of commonality with licensed PACS to permit economical licensed/unlicensed terminals and common network services. The results of our simulation show that, at 1% to 2% blocking probability, 99% of downlink local-mean signal-to-noise plus interference ratio (SINR) values are above 17 dB for a 10 to 20 m port separation. For a three-dimensional office environment, the uplink limits the SINR performance, however, with uplink power control, a 5.5 to 7 dB improvement in the uplink SINR can be achieved even for high traffic load.
Agents in an Integrated System Architecture (1997)

This paper presents research findings from development of an expert system and its
integration into an integrated environment. Expert systems has proven hard to integrate
because of their interactive nature. A prototype environment was developed using new
integration technologies, and research findings concerning the use of OLE technology to
integrate stand alone applications are discussed. The prototype shows clear advantages of
using OLE technology when developing integrated environments.
Teleconferencing comes of age: Electronic meetings enter a new era, thanks to personal computers, video printers, slide projectors, and bright graphics, among other tools (1984)

Recent improvements in teleconferencing are described. These include: a vastly improved microphone; echo cancellation; on-premises audio bridges; audio network bridges; and improved interactive graphics. Computer conferencing and interactive video conferencing are also discussed.
Fabrication and bonding of thiol-ene-based microfluidic devices (2013)

In this work, the bonding strength of microchips fabricated by thiol-ene free-radical polymerization was characterized in detail by varying the monomeric thiol/allyl composition from the stoichiometric ratio (1:1) up to 100% excess of thiol (2:1) or allyl (1:2) functional groups. Four different thiol-ene to thiol-ene bonding combinations were tested by bonding: (i) two stoichiometric layers, (ii) two layers bearing complementary excess of thiols and allyls, (iii) two layers both bearing excess of thiols, or (iv) two layers both bearing excess of allyls. The results showed that the stiffness of the cross-linked polymer plays the most crucial role regarding the bonding strength. The most rigid polymer layers were obtained by using the stoichiometric composition or an excess of allyls, and thus, the bonding combinations (i) and (iv) withstood the highest pressures (up to the cut-off value of 6 bar). On the other hand, excess of thiol monomers yielded more elastic polymer layers and thus decreased the pressure tolerance for bonding combinations (ii) and (iii). By using monomers with more thiol groups (e.g. tetrathiol versus trithiol), a higher cross-linking ratio, and thus, greater stiffness was obtained. Surface characterization by infrared spectroscopy confirmed that the changes in the monomeric thiol/allyl composition were also reflected in the surface chemistry. The flexibility of being able to bond different types of thiol-enes together allows for tuning of the surface chemistry to yield the desired properties for each application. Here, a capillary electrophoresis separation is performed to demonstrate the attractive properties of stoichiometric thiol-ene microchips.
Study of bending-induced strain effects on MuGFET performance (2006)

The impact of stress induced by biaxial mechanical bending on multiple-gate FET (MuGFET) performance is studied. For relatively low levels of bending-induced surface strain (/spl sim/ 0.1%), significant enhancements in the driving current can be achieved and maintained with gate-length scaling. This makes package strain a potentially attractive approach to enhancing MuGFET-based CMOS performance at low cost. For bending-induced strain, the enhancements in electron mobility and [110] hole mobility are well predicted by the piezoresistance model using the coefficients for bulk-Si, but the impact of stress on (100) hole mobility is more complex.
SL1 trans-splicing specified by AU-rich synthetic RNA inserted at the 5' end of Caenorhabditis elegans pre-mRNA. (1995)

In Caenorhabditis elegans, pre-mRNAs of many genes are trans-spliced to one of two spliced leaders, SL1 or SL2. Some of those that receive exclusively SL1 have been characterized as having at their 5' ends outrons, AU-rich sequences similar to introns followed by conventional 3' splice sites. Comparison of outrons from many different SL1-specific C. elegans genes has not revealed the presence of any consensus sequence that might encode SL1-specificity. In order to determine what parameters influence the splicing of SL1, we performed in vivo experiments with synthetic splice sites. Synthetic AU-rich RNA, 51 nt or longer, placed upstream of a consensus 3' splice site resulted in efficient trans-splicing. With all sequences tested, this trans-splicing was specifically to SL1. Thus, no information beyond the presence of AU-rich RNA at least as long as the minimum-length C. elegans intron, followed by a 3' splice site, is required to specify trans-splicing or for strict SL1 specificity.
The prognostic value of the hypoxia markers CA IX and GLUT 1 and the cytokines VEGF and IL 6 in head and neck squamous cell carcinoma treated by radiotherapy ± chemotherapy (2005)

To our knowledge, we are the first to report a link in HNSCC between IL-6 pretreatment serum levels and radioresistance in vivo. This link is supported by the strong prognostic association of pretreatment IL-6 with local control, known to be the most important parameter to judge radiotherapy responses. Furthermore, the combined assessment of CA IX and GLUT-1 correlated independently with prognosis. This is a valuable indication that a combined approach is important in the investigation of prognostic markers.
Multi-Step Adaptive Sensor Scheduling for Target Tracking in Wireless Sensor Networks (2006)

Sensor scheduling is essential to collaborative target tracking in Wireless Sensor Networks (WSNs). In this paper, we present a Multi-step Adaptive Sensor Scheduling algorithm (MASS) by selecting the next tasking sensor and its associated sampling interval based on the prediction of tracking accuracy and energy cost over a finite horizon of steps. MASS adopts alternative tracking mode for each prediction step, i.e., the fast tracking mode (FTM) or the tracking maintenance mode (TMM) dependent on whether the estimated or predicted tracking accuracy is satisfactory. The Best Sensor Schedule Sequence (BSSS) is found by searching and comparing the Candidate Sensor Schedule Sequences (CSSSs) at two levels, i.e., the logical tracking mode level which is simplely defined on multi-step tracking modes and the physical quantity performance level by considering the tradeoff between tracking accuracy and energy cost. MASS employs the extended Kalman filter (EKF) algorithm to predict the tracking accuracy and an energy consumption model to predict the energy cost. Simulation results show that, compared with the traditional non-adaptive sensor scheduling algorithm and the single-step adaptive sensor scheduling algorithm, MASS can achieve fast tracking speed and superior energy efficiency without degrading the tracking accuracy.
Characterization of CD44 splicing patterns in normal keratinocytes, dysplastic and squamous carcinoma cell lines (2001)

The CD44 glycoprotein is spliced from a complex gene of 10 constitutive and 10 variant exons. In this study, CD44 splicing patterns and intron 9 retention were investigated by exon-specific RT-PCR for variant exons v1-v10 and intron 9 in normal, immortalized, dysplastic and malignant keratinocytes. Expression of product was determined immunohistochemically for some of the exons. Normal keratinocytes showed one major transcript including exons v2-v10 and 3 minor transcripts. No lines showed a normal CD44 splicing pattern but rather a variety of truncated transcripts of contiguous variant exons which overall correlated with expression. Squamous cell carcinoma (SCC)-4 and SCC-9 lines showed relatively normal transcripts although protein was expressed only by SCC-9. SCC-12B2, SCC-15, SCC-25 and SCC-27 showed a series of shorter overlapping transcripts, with loss of exons v8-v10 in the major transcripts. Intron 9 was not retained in normal keratinocytes or cell lines. Despite the fact that keratinocytes constitutively express all variant exons, splicing patterns are distinctly abnormal and merit investigation as potential markers for epidermal and oral squamous malignancy.
Discovery of Cdc25A Lead Inhibitors with a Novel Chemotype by Virtual Screening: Application of Pharmacophore Modeling Based on a Training Set with a Limited Number of Unique Components (2017)

Cdc25 phosphatase was studied as an attractive target for cancer therapy. Multiple pharmacophore models with the unique core features of classic quinone inhibitors and those of novel inhibitors were used to discover a novel lead inhibitor. A total of 21 compounds with qualified physical properties were screened from the Maybridge HitFinder database containing 14 400 compounds by pharmacophore models. Four compounds were found to inhibit Cdc25A activity by more than 50 % at a concentration of 100 μm. Among these compounds, KM10389 (N-{2-[(furan-2-ylmethyl)thio]ethyl}-2-[(4-hydroxy-6-propylpyrimidin-2-yl)thio]acetamide) showed high inhibitory activity with an IC<sub>50</sub> value of 7.9 μm. Selective cytotoxicity toward HeLa cells was observed with an IC<sub>50</sub> value of 66.3 μm, whereas the IC<sub>50</sub> value for HEK293 cells was higher than 100 μm. Blocking of the G1/S transition was also observed for HeLa cells in the presence of the compound by increasing the G1 phase by 16.15 %. Together with compounds HTS02435 and HTS01205, a novel lead inhibitor structure was identified and analyzed by a molecular docking study. The implication of virtual screening by using different pharmacophore models representing the different features is fully discussed.
Essential activation of PKC-δ in opioid-initiated cardioprotection (2001)

Stimulation of the delta(1)-opioid receptor confers cardioprotection to the ischemic myocardium. We examined the role of protein kinase C (PKC) after delta-opioid receptor stimulation with TAN-67 or D-Ala(2)-D-Leu(5)-enkephalin (DADLE) in a rat model of myocardial infarction induced by a 30-min coronary artery occlusion and 2-h reperfusion. Infarct size (IS) was determined by tetrazolium staining and expressed as a percentage of the area at risk (IS/AAR). Control animals, subjected to ischemia and reperfusion, had an IS/AAR of 59.9 +/- 1.8. DADLE and TAN-67 administered before ischemia significantly reduced IS/AAR (36.9 +/- 3.9 and 36.7 +/- 4.7, respectively). The delta(1)-selective opioid antagonist 7-benzylidenenaltrexone (BNTX) abolished TAN-67-induced cardioprotection (54.4 +/- 1.3). Treatment with the PKC antagonist chelerythrine completely abolished DADLE- (61.8 +/- 3.2) and TAN-67-induced cardioprotection (55.4 +/- 4.0). Similarly, the PKC antagonist GF 109203X completely abolished TAN-67-induced cardioprotection (54.6 +/- 6.6). Immunofluorescent staining with antibodies directed against specific PKC isoforms was performed in myocardial biopsies obtained after 15 min of treatment with saline, chelerythrine, BNTX, or TAN-67 and chelerythrine or BNTX in the presence of TAN-67. TAN-67 induced the translocation of PKC-alpha to the sarcolemma, PKC-beta(1) to the nucleus, PKC-delta to the mitochondria, and PKC-epsilon to the intercalated disk and mitochondria. PKC translocation was abolished by chelerythrine and BNTX in TAN-67-treated rats. To more closely examine the role of these isoforms in cardioprotection, we utilized the PKC-delta selective antagonist rottlerin. Rottlerin abolished opioid-induced cardioprotection (48.9 +/- 4.8) and PKC-delta translocation without affecting the translocation of PKC-alpha, -beta(1), or -epsilon. These results suggest that PKC-delta is a key second messenger in the cardioprotective effects of delta(1)-opioid receptor stimulation in rats.
Ligand-Promoted <i>meta</i>-C–H Amination and Alkynylation (2016)

Using a modified norbornene (methyl bicyclo[2.2.1]hept-2-ene-2-carboxylate) as a transient mediator, meta-C-H amination and meta-C-H alkynylation of aniline and phenol substrates have been developed for the first time. Both the identification of a monoprotected 3-amino-2-hydroxypyridine/pyridone-type ligand and the use of a modified norbornene as a mediator are crucial for the realization of these two unprecedented meta-C-H transformations. A variety of substrates are compatible with both meta-C-H amination and meta-C-H alkynylation. Amination and alkynylation of heterocyclic substrates including indole, indoline, and indazole afford the desired products in moderate to high yields.
A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex (2000)

The microtubule motor cytoplasmic dynein performs multiple cellular functions; however, the regulation and targeting of the motor to different cargoes is not well understood. A biochemical interaction between the dynein intermediate chain subunit and the p150-Glued component of the dynein regulatory complex, dynactin, has supported the hypothesis that the intermediate chain is a key modulator of dynein attachment to cellular cargoes. In this report, we identify multiple intermediate chain polypeptides that cosediment with the 19S dynein complex and two differentially expressed transcripts derived from the single cytoplasmic dynein intermediate chain (Cdic) gene that differ in the 3' untranslated region sequence. These results support previous observations of multiple Cdic gene products that may contribute to the specialization of dynein function. Most significantly, we provide genetic evidence that the interaction between the dynein intermediate chain and p150-Glued is functionally relevant. We use a genomic Cdic transgene to show that extra copies of the dynein intermediate chain gene act to suppress the rough eye phenotype of the mutant Glued(1), a mutation in the p150-Glued subunit of dynactin. Furthermore, we show that the interaction between the dynein intermediate chain and p150-Glued is dependent on the dosage of the Cdic gene. This result suggests that the dynein intermediate chain may be a limiting component in the assembly of the dynein complex and that the regulation of the interaction between the dynein intermediate chain and dynactin is critical for dynein function.
Identification of a pathogenic epitope involved in initiation of Heymann nephritis. (1992)

Heymann nephritis is an experimental autoimmune disease model for human membranous nephropathy. We have recently identified a pathogenic epitope, clone 14 (C14), responsible for formation and deposition of glomerular immune complexes that is contained within the small subunit of the Heymann nephritis antigenic complex (HNAC). HNAC is a heterodimer composed of a large subunit designated gp330 and a smaller (44 kDa) subunit, which is immunologically identical to the receptor-associated protein. In this study, we prepared antibodies to fusion proteins with C-terminal deletions in the C14 sequence and assessed their ability to promote formation of immune deposits (IDs). When IgG specific for the shortest truncated fusion protein (C14/delta 3; 86 amino acids) was injected into rats, small IDs developed. In contrast, when IgG raised against the full-length C14 sequence was depleted of its reactivity toward the C14/delta 3 fusion protein (C14/delta 3-fp), no IDs could be detected. These data indicate that at least one pathogenic epitope is contained within the N-terminal 86 amino acids of C14. Since the IDs induced with the C14/delta 3-fp-specific IgG are smaller than those induced with the poly-epitope-specific anti-gp330 antibodies, it is likely that other epitopes in addition to those expressed by the C14/delta 3-fp are required for formation and growth of immune complexes.
Statistical learning approach for predicting specific pharmacodynamic, pharmacokinetic, or toxicological properties of pharmaceutical agents (2005)

Abstract Pharmaceutical agents have been developed and tested for possessing desirable pharmacodynamic, pharmacokinetic, and minimal level of toxicological properties. Computational methods have been explored for predicting these properties aimed at the discovery of promising leads and the elimination of unsuitable ones in early stages of drug development. Statistical learning methods have shown their potential for predicting these properties for structurally diverse sets of agents by using both conventional (quantitative structure–activity and structure–property relationships) and more recently explored (such as neural networks and support vector machines) statistical models. These methods have been used for predicting agents of a variety of pharmacodynamic (such as inhibitors or agonists of a therapeutic target), pharmacokinetic (such as P‐glycoprotein substrates, human intestine absorption, and blood–brain barrier penetrating capabilities), and toxicological (such as genotoxicity) properties. The strategies, current progresses, and the underlying difficulties and future prospects of the application of the recently explored statistical learning methods are discussed. Drug Dev. Res. 66:245–259, 2006. © 2006 Wiley‐Liss, Inc.
In Vitro Replication Kinetics of Human Immunodeficiency Virus Type 1 (HIV‐1) Variants in Relation to Virus Load in Long‐Term Survivors of HIV‐1 Infection (1998)

In 7 long-term survivors (LTS) and 8 progressors, all carrying solely non-syncytium-inducing variants, a possible correlation between in vitro virus replicative capacity, virus load, and clinical course of human immunodeficiency virus type 1 (HIV-1) infection was analyzed. Late in infection, 3 LTS and 7 progressors had a high virus load, which coincided with the presence of rapid-replicating viruses. In contrast to progressors, LTS maintained relatively high and stable CD4 T cell counts. Four LTS persistently had relatively slow-replicating viruses and a low virus load, even after 6.6-9 years of seropositive follow-up. All virus isolates from 1 of these LTS had a 4-aa deletion in nef. These results suggest a correlation between the in vitro replicative capacity of non-syncytium-inducing HIV-1 variants and virus load. The presence of HIV-1 variants with relatively low replicative capacity throughout infection may have contributed to the beneficial clinical course in half of the LTS in this study.
A fully integrated multi-output CMOS frequency synthesizer for channelized receivers (2004)

A fully integrated frequency synthesizer that generates multiple frequencies for use in frequency channelized receivers is designed in 0.25 /spl mu/m CMOS technology. Many of the unique features of this receiver have been exploited to design a highly integrated and power efficient multi-output frequency synthesizer, including poly-phase filters. The synthesizer generates 4, 5, 6, and 7 GHz signals while dissipating 120 mW.