gebhart/patrepeval huggingface dataset:
Enhanced anti‐tumor effects of the PD‐1 blockade combined with a highly absorptive form of curcumin targeting STAT3 (2020)

PD-1/PD-L1 immune checkpoint inhibitors are promising cancer immunotherapies however responses are still limited and the development of more effective combination immunotherapy is needed. We previously reported that STAT3 activation in cancer cells and immune cells was involved in immune-resistant mechanisms. In this study, we evaluated the effect of highly absorptive forms of curcumin extracts and synthetic curcumin on anti-tumor T cell responses. The curcumin po administration resulted in the significant augmentation of in vivo induction of tumor antigen-specific T cells through restoration of dendritic cells (DCs) by inhibiting directly STAT3 in DCs and indirectly via reduced IL-6 production from STAT3 activated cancer cells in 2 syngeneic MC38 and CT26 murine tumor models. Curcumin also showed direct DC enhancing activity and enhanced T cell induction for the immunized antigens in non-tumor-bearing mice and human hosts. Curcumin restored DC functions in xenogeneic nude mouse model implanted with high IL-6-producing human clear cell ovarian cancer cells. The combination of curcumin and PD-1/PD-L1 Abs demonstrated a synergistic anti-tumor activity in MC38 murine tumor models. These results indicated that curcumin augments the induction of tumor antigen-specific T cells by restoring the T cell stimulatory activity of DCs targeting activated STAT3 in both cancer cells and immune cells. Combination immunotherapy with curcumin and PD-1/PD-L1 Ab is an attractive strategy in the development of effective immunotherapy against various cancers.
Advertising alcohol when the best isn't good enough (2006)

Since 1998 alcohol advertising in Australia has been governed by the Alcohol Beverages Advertising Code (ABAC). ABAC is a voluntary, self-regulatory convention administered by four industry associations: the Distilled Spirits Industry Council, Australian Associated Brewers, the Liquor Merchants' Association and the Winemakers' Federation.
Mechanisms of TGF-β-Induced Cell Cycle Arrest (1998)

Mitogenic growth factors stimulate cell growth by initiating a signaling cascade leading to the activation of the cyclin-dependent kinases (cdks), phosphorylation of pRb, and subsequent entry of the cell into the S phase. Transforming growth factor-beta (TGF-beta) is a potent antimitogen in a wide variety of cells and is postulated to inhibit cell cycle progression by blocking the late G1 activation of the cdks, thereby preventing pRb phosphorylation and S phase entry. The loss of TGF-beta sensitivity in many transformed cells coupled with recent data demonstrating a deregulation of cyclins, cdks, and cdk inhibitors in many types of cancer has attracted much attention to the molecular mechanism of TGF-beta-mediated growth arrest. Despite these recent advances, further research is required to elucidate how these effects of TGF-beta on the cyclins, cdks, and cdk inhibitors are linked to the TGF-beta receptor complex and the Smad proteins.
Micromanipulation Studies of Chromatin Fibers in <i>Xenopus</i> Egg Extracts Reveal ATP-dependent Chromatin Assembly Dynamics (2006)

We have studied assembly of chromatin using Xenopus egg extracts and single DNA molecules held at constant tension by using magnetic tweezers. In the absence of ATP, interphase extracts were able to assemble chromatin against DNA tensions of up to 3.5 piconewtons (pN). We observed force-induced disassembly and opening-closing fluctuations, indicating our experiments were in mechanochemical equilibrium. Roughly 50-nm (150-base pair) lengthening events dominated force-driven disassembly, suggesting that the assembled fibers are chiefly composed of nucleosomes. The ATP-depleted reaction was able to do mechanical work of 27 kcal/mol per 50 nm step, which provides an estimate of the free energy difference between core histone octamers on and off DNA. Addition of ATP led to highly dynamic behavior with time courses exhibiting processive runs of assembly and disassembly not observed in the ATP-depleted case. With ATP present, application of forces of 2 pN led to nearly complete fiber disassembly. Our study suggests that ATP hydrolysis plays a major role in nucleosome rearrangement and removal and that chromatin in vivo may be subject to highly dynamic assembly and disassembly processes that are modulated by DNA tension.
Iridium‐dispersed carbon paste enzyme electrodes (1996)

Abstract An highly selective first‐generation glucose biosensor, based on an iridium‐dispersed carbon‐paste transducer, is described. The iridium‐on‐carbon particles provide excellent electrocatalysis for both the reduction and oxidation of hydrogen peroxide. Such unique catalytic action offers convenient biosensing of glucose at very low operating potentials, where interfering reactions are negligible. Factors influencing the electrocatalytic and biocatalytic actions are elucidated, along with the analytical performance. Implications to other oxidase‐based amperometric electrodes are discussed.
Micronized Palmitoylethanolamide Reduces the Symptoms of Neuropathic Pain in Diabetic Patients (2014)

The present study evaluated the effectiveness of micronized palmitoylethanolamide (PEA-m) treatment in reducing the painful symptoms experienced by diabetic patients with peripheral neuropathy. PEA-m, a fatty acid amide of the N-acylethanolamine family, was administered (300 mg twice daily) to 30 diabetic patients suffering from painful diabetic neuropathy. Before treatment start, after 30 and 60 days the following parameters were assessed: painful symptoms of diabetic peripheral neuropathy using the Michigan Neuropathy Screening instrument; intensity of symptoms characteristic of diabetic neuropathic pain by the Total Symptom Score; and intensity of different subcategories of neuropathic pain by the Neuropathic Pain Symptoms Inventory. Hematological and blood chemistry tests to evaluate metabolic control and safety were also performed. Statistical analysis (ANOVA) indicated a highly significant reduction in pain severity (P < 0.0001) and related symptoms (P < 0.0001) evaluated by Michigan Neuropathy Screening instrument, Total Symptom Score, and Neuropathic Pain Symptoms Inventory. Hematological and urine analyses did not reveal any alterations associated with PEA-m treatment, and no serious adverse events were reported. These results suggest that PEA-m could be considered as a promising and well-tolerated new treatment for symptomatology experienced by diabetic patients suffering from peripheral neuropathy.
Recycling of waste printed circuit boards (2010)

Purpose – The purpose of this paper is to present an update and the latest results from work on a project aimed at enabling waste printed circuit board (WPCB) recycling to become more environmentally friendly and efficient.
Reduction of Protein Tyrosine Phosphatase 1B Increases Insulin-Dependent Signaling in <i>ob/ob</i> Mice (2003)

Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin receptor (IR) signal transduction and a drug target for treatment of type 2 diabetes. Using PTP1B antisense oligonucleotides (ASOs), effects of decreased PTP1B levels on insulin signaling in diabetic ob/ob mice were examined. Insulin stimulation, prior to sacrifice, resulted in no significant activation of insulin signaling pathways in livers from ob/ob mice. However, in PTP1B ASO-treated mice, in which PTP1B protein was decreased by 60% in liver, similar stimulation with insulin resulted in increased tyrosine phosphorylation of the IR and IR substrate (IRS)-1 and -2 by threefold, fourfold, and threefold, respectively. IRS-2-associated phosphatidylinositol 3-kinase activity was also increased threefold. Protein kinase B (PKB) serine phosphorylation was increased sevenfold in liver of PTP1B ASO-treated mice upon insulin stimulation, while phosphorylation of PKB substrates, glycogen synthase kinase (GSK)-3alpha and -3beta, was increased more than twofold. Peripheral insulin signaling was increased by PTP1B ASO, as evidenced by increased phosphorylation of PKB in muscle of insulin-stimulated PTP1B ASO-treated animals despite the lack of measurable effects on muscle PTP1B protein. These results indicate that reduction of PTP1B is sufficient to increase insulin-dependent metabolic signaling and improve insulin sensitivity in a diabetic animal model.
Differential expression of multiple genes during articular chondrocyte redifferentiation (2001)

Articular chondrocytes undergo a rapid change in phenotype and gene expression, termed dedifferentiation, when isolated from cartilage tissue and cultured on tissue culture plastic. On the other hand, "redifferentiation" of articular chondrocytes in suspension culture is characterized by decreased cellular proliferation and the reinitiation of synthesis of hyaline articular cartilage extracellular matrix molecules. The molecular triggers for these events have yet to be defined. Subtracted cDNA libraries representing genes involved in the early events of adult human articular chondrocyte redifferentiation were generated from human articular chondrocytes that were first cultured in monolayer, and subsequently transferred to suspension culture at 10(6) cells/ml for redifferentiation. Differential regulation of genes involved in cellular organization, nuclear structure, cellular growth regulation, and extracellular matrix deposition and remodeling were observed within 48 hr of this transfer. Many of these genes had not been previously identified in the chondrocyte differentiation pathway and a number of the isolated cDNAs did not have homologies to sequences in the public data banks. Genes involved in IL-6 signal transduction including acute phase response factor (APRF), Mn superoxide dismutase, and IL-6 itself were up-regulated in suspension culture. Membrane glycoprotein gp130, a component of the IL-6 receptor, was down-regulated. Other genes involved in cell polarity, cell adherence, apoptosis, and possibly TGF-beta signaling were differentially regulated. The differential regulation of the cytokine connective tissue growth factor (CTGF) during the early stages of articular chondrocyte redifferentiation, decreasing within 48 hours of transfer to suspension culture, was particularly interesting given its reported role in the stimulation of cellular proliferation. CTGF was highly expressed in proliferative monolayer culture, and then greatly reduced by redifferentiation in standard high-density suspension culture. When articular chondrocytes were seeded in suspension at low-density (10(4) cells/ml), however, high levels of CTGF were observed along with increased levels of mature articular cartilage extracellular matrix protein RNAs, such as type II collagen and aggrecan. Although the role of CTGF in articular cartilage biology remains to be elucidated, the results described here demonstrate the potential utility of subtractive hybridization in understanding the process of articular chondrocyte redifferentiation.
Downregulation of L-arginine metabolism in dendritic cells induces tolerance to exogenous antigen (2016)

Dendritic cells (DC) are potential tools for therapeutic applications and several strategies to generate tolerogenic DCs are under investigation. When activated by cytokines and microbial products, DCs express mediators that modulate immune responses. In this regard, the metabolites generated by the activities of inducible nitric oxide synthase (iNOS) and arginase in DCs seem to play important roles. Here, we evaluated the effects of adoptive transfer of DCs generated in vitro from bone marrow precursors (BMDC) modulated with L-NAME (Nω-nitro-L-arginine methyl ester) and NOHA (NG-Hydroxy-L-arginine), inhibitors of iNOS and arginase, respectively, upon the immune response of the wild type (BALB/c) and OVA-TCR transgenic (DO11.10) mice. The modulation with L-NAME increased CD86 expression in BMDC, whereas treatment with NOHA increased both CD80 and CD86 expression. Adoptive transfer of either L-NAME- or NOHA-modulated BMDCs to BALB/c mice reduced the plasma levels of ovalbumin-specific antibody as well as proliferation and cytokine secretion in cultures of spleen cells in comparison adoptive transfer of non-modulated DCs. Conversely, transfer of both modulated and non-modulated BMDCs had no effect on immune response of DO11.10 mice. Together, these results show that the treatment with iNOS and Arg inhibitors leads to increased expression of co-stimulatory molecules in DCs, and provides evidences that L-arginine metabolism may be an important therapeutic target for modulating immune responses in inflammatory disorders.
Phospholipases A<sub>2</sub>: structure and function (2005)

Phospholipases A2 (PLA2) are an example of peripheral membrane proteins that must first bind to the phospholipid interface to allow phospholipid hydrolysis to occur. The interfacial (membrane) binding step plays a crucial role in the biological function of the enzyme and membrane affinity may be determined both by the phospholipid composition of the membrane and the properties of the interfacial binding surface of the protein. There are now three major categories of these enzymes, secreted PLA2 (sPLA2), cytosolic PLA2 (cPLA2) and Ca2+-independent PLA2 (iPLA2). The structure and function of each category is discussed highlighting how both membrane binding and phospholipid substrate specificity may contribute to the overall functions of these enzymes.
Preparation and characteristics of calcium halophosphates (1955)

After a brief survey of the characteristics of the important antimony/manganese activated calcium halophosphates, a more detailed description is given of the dry and wet syntheses. The colour characteristics and the theoretical and attained efficiencies are discussed with reference to three halophosphates, the light blue, the daylight and the warm white. Maintenance of light output of fluorescent lamps, and the improvement of lamp colour by admixture with other phosphors, are also considered.
4th International Conference on Gynecology and Obstetrics Pathology (2019)

Awards 2020
We are pleased to announce 4th International Conference on Gynecology and Obstetrics Pathology which is going to be held in Munich, Germany on May 11-12, 2020. Conference aims to bring together the prominent researchers, academic scientists, and research scholars to exchange and share their experiences on all aspects of Gynecology and Obstetrics.
Generation of Rx <sup>+</sup> /Pax6 <sup>+</sup> neural retinal precursors from embryonic stem cells (2005)

We report directed differentiaion of retinal precursors in vitro from mouse ES cells. Six3+ rostral brain progenitors are generated by culturing ES cells under serum-free suspension conditions (SFEB culture) in the presence of Wnt and Nodal antagonists (Dkk1 and LeftyA), and subsequently steered to differentiate into Rx+ cells (16%) by treatment with activin and serum. Consistent with the characteristics of early neural retinal precursors, the induced Rx+ cells coexpress Pax6 and the mitotic marker Ki67, but not Nestin. The ES cell-derived precursors efficiently generate cells with the photoreceptor phenotype (rhodopsin+, recoverin+) when cocultured with embryonic retinal cells. Furthermore, organotypic culture studies demonstrate the selective integration and survival of ES cell-derived cells with the photoreceptor phenotype (marker expression and morphology) in the outer nuclear layer of the retina. Taken together, ES cells treated with SFEB/Dkk1/LeftyA/serum/activin generate neural retinal precursors, which have the competence of photoreceptor differentiation.
Performance of Hydraulic Gas Compressors (1976)

A hydraulic gas compressor has no moving parts and compresses gas at the expense of hydraulic energy by means of entrainment in a downward moving column of liquid with subsequent separation of gas and liquid at depth. Documentation is cited giving the history and performance data for large hydraulic air compressors built around 1900. An analysis is presented based on one-dimensional bulk parameter modeling of the flow, which enables computerized calculation of the performance of hydraulic gas compressors. The results of small-scale experiments are presented showing agreement with analytical results. It is further shown that the analytical results agree with reported performance data for old large-scale compressors. Unique capabilities of the compressor for transducing and transforming energy are described and application studies are suggested in several areas. Calculated results are presented as an example, for the design and performance of a large air compressor, showing the effect on performance of varying the applied hydraulic head.
A comparison between the M-algorithm and the list Viterbi algorithm (2008)

In this paper we compare the performances of the M-algorithm (MA) and the list Viterbi algorithm (LVA) for a concatenated coding scheme with outer cyclic redundancy code (CRC) and inner convolutional code. Even though the Viterbi algorithm (VA) performs the optimum decoding for convolutional codes, it is sub-optimum for the concatenated system. The LVA provides an ordered list of decoded bit sequences by embedding limited list search in its trellis structure. On the contrary, the MA is a list-based algorithm which can be viewed as a constrained tree search decoder. Our results show that for the considered concatenated system, list-based algorithm outperforms trellis-based algorithm for equivalent complexity. A set of simulation results are presented comparing these algorithms for different constraint lengths, code rates and channel conditions.
Collagens and proteoglycans of the corneal extracellular matrix (2003)

The cornea is a curved and transparent structure that provides the initial focusing of a light image into the eye. It consists of a central stroma that constitutes 90% of the corneal depth, covered anteriorly with epithelium and posteriorly with endothelium. Its transparency is the result of the regular spacing of collagen fibers with remarkably uniform diameter and interfibrillar space. Corneal collagen is composed of heterotypic fibrils consisting of type I and type V collagen molecules. The cornea also contains unusually high amounts of type VI collagen, which form microfibrillar structures, FACIT collagens (XII and XIV), and other nonfibrillar collagens (XIII and XVIII). FACIT collagens and other molecules, such as leucine-rich repeat proteoglycans, play important roles in modifying the structure and function of collagen fibrils.Proteoglycans are macromolecules composed of a protein core with covalently linked glycosaminoglycan side chains. Four leucine-rich repeat proteoglycans are present in the extracellular matrix of corneal stroma: decorin, lumican, mimecan and keratocan. The first is a dermatan sulfate proteoglycan, and the other three are keratan sulfate proteoglycans. Experimental evidence indicates that the keratan sulfate proteoglycans are involved in the regulation of collagen fibril diameter, and dermatan sulfate proteoglycan participates in the control of interfibrillar spacing and in the lamellar adhesion properties of corneal collagens. Heparan sulfate proteoglycans are minor components of the cornea, and are synthesized mainly by epithelial cells. The effect of injuries on proteoglycan synthesis is discussed.
Un enfoque clínico terapéutico para la alopecia androgenética: una descripción general (2022)

Introducción: La alopecia es una entidad importante por su frecuencia y ser origen de conflictos emocionales. La alopecia androgenética es la progresión fisiológica de los folículos pilosos del cuero cabelludo. Consti- tuye la principal indicación del trasplante capilar tanto en hombres como en mujeres. En los últimos años el hombre se ha preocupado más por su apariencia física y ha aumentado el número de pacientes del sexo mascu- lino que acuden a consulta en búsqueda de corrección de la calvicie que en su mayoría es de causa androgénica, esto ha llevado al perfecciona- miento de las técnicas de trasplante capilar. Objetivo: Profundizar en el diagnóstico de la alopecia androgenética, las opciones de tratamiento y sus resultados. Desarrollo: Existen varias opciones de tratamiento como medicamentos tópicos o infiltrativos, terapia regenerativa con plasma rico en plaquetas y células madre mesenquimales, fototerapia con LED tem- poral o permanente, crioterapia, micropigmentación capilar, dispositivos como pelucas y extensiones, y técnicas quirúrgicas que incluyen escisio- nes múltiples, rotación de colgajos, expansión tisular y el trasplante capi- lar. Conclusiones: La alopecia androgenética, es una de las consultas en Cirugía Plástica más solicitada en los hombres, superada solo por otros procedimientos como la rinoplastia y la blefaroplastia.
Three-stage birefringent filter tuning smoothly over the visible region: theoretical treatment and experimental design (1980)

The Jones transformation matrix, which describes the arbitrary incidence of polarized light on a thin uniaxial birefringent plate, is determined. With the optic axis presumed to lie in the plane of the plate, digital solutions to the resulting formulas are presented for several different quartz plates to depict the tuning characteristics of a birefringent plate functioning as a single-stage birefringent filter. A simplified model is used to generalize these results to describe a multistage birefringent filter. This theoretical study forms the basis for the design and construction of a three stage, quartz, birefringent filter to be used as the coarse tuning element in a visible cw dye laser. A relatively simple and straightforward procedure for the assembly of the three-stage filter is given. The performance of this newly constructed filter is found to be superior to currently available commercial models, tuning smoothly over the 4400-6600-A wavelength region.
Worthy of Attention: Accounting Manual for Colleges (1938)

"Worthy of Attention: Accounting Manual for Colleges." The Journal of Higher Education, 9(5), pp. 288–289
Uninterruptible power supplies: classification, operation, dynamics, and control (2003)

This paper presents a comprehensive review of uninterruptible power supply (UPS) systems in terms of topologies, operation, dynamics and control. UPS systems are classified with emphasis on static systems. This paper also addresses fundamental problems faced in these systems in different distributed and centralized applications. In addition, a brief description of the conventional UPS systems, their disadvantages, scope for improvement, advanced architectures, role of power electronics and current trends in UPS research are explained. Negative impedance dynamics in UPS systems with constant power loads are also introduced. Finally, this paper concludes with a brief outline of the advancement to be made in the future.