gebhart/patrepeval huggingface dataset:
ALCOHOL DOES NOT MODULATE THE AUGMENTED ACETYLCHOLINE-INDUCED VASODILATORY RESPONSE IN HEMORRHAGED RODENTS (2009)

Our previous studies have shown that acute alcohol intoxication (AAI) decreases blood pressure, exacerbates hypotension after hemorrhagic shock, impairs the pressor response to fluid resuscitation, and blunts neuroendocrine activation. We hypothesized that impaired hemodynamic compensation during and after hemorrhagic shock in the acute alcohol-intoxicated host is the result of blunted neuroendocrine activation or, alternatively, of an impaired vascular responsiveness to vasoactive agents. The aim of this study was to examine the effects of AAI, AAI and hemorrhagic shock, and AAI and hemorrhagic shock and resuscitation on reactivity of isolated blood vessel rings to phenylephrine and acetylcholine. Chronically instrumented, conscious male Sprague-Dawley rats (300-350 g) received a primed continuous 15-h intragastric alcohol infusion (2.5 g x kg(-1) + 300 mg x kg(-1) x h(-1)), and time-matched controls received an isocaloric-isovolumic dextrose infusion. At completion of infusions, animals were randomized to sham, 60-min fixed-pressure hemorrhage, or hemorrhagic shock followed by resuscitation with lactated Ringer's solution. At the completion of the experimental protocols, animals were killed, and thoracic aorta and mesenteric artery ring segments (1-2 mm) were prepared and studied in myograph baths. Acute alcohol intoxication did not produce significant alterations in either pressor or dilator responses in aortic or mesenteric rings. These findings suggest that impaired hemodynamic counterregulation during hemorrhagic shock in AAI is not due to decreased vasopressor responsiveness. However, our results suggest a role for accentuated vasodilatory responses that may be central in progression to decompensatory shock.
Diversity of the envelope glycoprotein among human immunodeficiency virus type 1 isolates of clade E from Asia and Africa (1996)

Human immunodeficiency virus type 1 isolates of clade E, known to be largely responsible for the fulminating epidemic in Southeast Asia, have been derived exclusively from Asia and Africa. Here we provide full or partial sequences of the envelope glycoprotein gene from 13 additional clade E isolates from Asia representing patients in both early and late stages of disease. More extensive comparison of isolates within clade E by geographic locale, stage of disease, and year of isolation is now possible. The genetic diversity of clade E isolates from Asia, particularly among those derived from early-stage patients, is restricted compared with African isolates (mean interisolate distances in gp120, 5.4 and 20.2%, respectively). However, patients hospitalized with AIDS-related illnesses in Thailand harbored clade E isolates exhibiting broader interisolate diversity and with highly heterogeneous third hypervariable loop sequences. An additional pair of cysteine residues, predicting a novel disulfide bridge and present in 80% of clade E isolates from Asia, was uniformly absent from six African isolates. Clade E isolates in Thailand from early-stage subjects continue to be genetically similar to potential vaccine prototype strains, providing a favorable environment for the evaluation of genotype E candidate vaccines. However, evidence of increasing interisolate diversity is appearing among late-stage patients in Asia. This diversification of the clade E virus, if sustained, may impact preventive vaccine development strategies.
<i>Luc</i> genes: Introduction of colour into bioluminescence assays (1990)

Luminescence assays are generally based on measurements of light intensity alone. Inclusion of colour as an additional parameter of the assay could increase the information content. Colour variation in luminescence is particularly prevalent among beetle luciferases. To study the relationship between enzyme structure and colour, luciferases from a Jamalcan click beetle were examined as a model system. These luciferases emit light ranging from green to orange, though their amino acid sequences differ by less than 5%. Through mutation of their respective cDNA clones, the amino acids responsible for the colour variation were identified. These specific amino acids are few, and they act upon colour independently with respect to the enzyme structure. Analysis of their effects indicates that the potential for colour variation among beetle luciferases is greater than is evident among the click beetle luciferase. Because of the subtle changes of enzyme structure that effect colour, luciferases that emit different colours may be useful as paired genetic reporters. They should interact equivalently with the intracellular environment of a host, but could be distinguished by colour in their assay. Such paired reporters could be used to observed simultaneous events, or to provide internal control for luminescence measurements.
The Pros and Cons of Anti-Aging Medicine (2004)

The Pros and Cons of Anti-Aging Medicine Get access Robert.B Hudson Robert.B Hudson Editor Search for other works by this author on: Oxford Academic Google Scholar Public Policy & Aging Report, Volume 14, Issue 2, Spring 2004, Page 2, https://doi.org/10.1093/ppar/14.2.2 Published: 01 March 2004
Friction at a Lubricated Line Contact Operating at Oscillating Sliding Velocities (1990)

Although many contacts operate under unsteady loading and sliding conditions, friction behavior under such conditions is still not well understood. In this paper we report on a series of experiments that were run to examine friction-velocity characteristics of line contacts operating under unsteady sliding velocities in the mixed, elastohydrodynamic and hydrodynamic lubrication regimes. A periodic, time-varying velocity component was superimposed on a steady sliding speed in such a way that all three lubrication regimes could be covered in a cycle. It was found that as the frequency of oscillation was increased, a multi-valued friction coefficient appeared as a loop about the average (steady state) friction-velocity relation. It is shown that this behavior can be modeled by a characteristic time lag between a changing velocity and the corresponding steady state friction. The latter is described by a single equation that was matched to measured average friction data. In the mixed lubrication regime, which is where this lag most significantly affects friction behavior, the lag time increases with normal load and lubricant viscosity. It is shown that the time shift is not associated with a fixed characteristic distance. The observed delay arises due to entrainment and normal approach, which includes squeeze-films combined with rough surface contact deformations.
Quantum Confined Stark Effect in Single CdSe Nanocrystallite Quantum Dots (1998)

The quantum-confined Stark effect in single cadmium selenide (CdSe) nanocrystallite quantum dots was studied. The electric field dependence of the single-dot spectrum is characterized by a highly polarizable excited state (∼10 5 cubic angstroms, compared to typical molecular values of order 10 to 100 cubic angstroms), in the presence of randomly oriented local electric fields that change over time. These local fields result in spontaneous spectral diffusion and contribute to ensemble inhomogeneous broadening. Stark shifts of the lowest excited state more than two orders of magnitude larger than the linewidth were observed, suggesting the potential use of these dots in electro-optic modulation devices.
Jugular Bulb Oxygen Saturation Monitoring for Evaluating Cerebral Ischemia (1994)

Traumatic brain injury challenges the health care team to employ treatment modalities to prevent secondary brain damage. Initially, the focus was on controlling intracranial pressure and maintaining cerebral perfusion pressure. It is now known it is more critical to determine whether flow matches the brain's metabolic needs as indicated by the cerebral metabolic rate of oxygen. Measuring cerebral metabolic rate of oxygen, the brain's oxygen consumption, is not attained easily due to technical reasons; therefore, it is calculated from more easily attained values (cerebral blood flow x arteriovenous oxygen content difference [AVDO2]). Continuous monitoring of cerebral venous O2 saturation is accomplished via the jugular vein. In most incidences where arterial O2 saturation is stable, then AVDO2 is inversely proportional to the jugular bulb O2 saturation. Jugular bulb O2 saturation, and therefore AVDO2, can be used to evaluate cerebral O2 supply and demand balance as it correlates with cerebral blood flow, with important exceptions as discussed. The most immediate value of jugular bulb O2 saturation monitoring lies in its ability to guide the effects of therapy on O2 supply and demand balance.
Therapeutic Monitoring of Tacrolimus Concentrations in Blood: Semi-Automated Extraction and Liquid Chromatography-Electrospray Ionization Mass Spectrometry (2001)

The authors describe a highly selective liquid chromatographic/mass spectrometric (LC/MS) method for measurement of the immunosuppressive drug tacrolimus. A protein-free supernatant of a patient's whole-blood sample (500 microL) is applied to an Empore styrene-divinylbenzene (SDB-XC) disk cartridge (Varian Sample Preparation Products; Harbor City, CA) to isolate tacrolimus and the internal standard, ascomycin. The entire extraction is automated on the Gilson ASPEC XL4 (Gilson; Middleton, WI). Separation takes place on an octyldecyl (C18) high-performance liquid chromatographic (HPLC) column maintained at 75 degrees C with a simple mobile phase of acetonitrile/water (90/10, v/v). An atmospheric pressure ionization (API)-electrospray ionization(ESI)-mass spectrometer (MS) is used for analysis. Detection is by selected ion monitoring (SIM) of the positively charged sodium adduct of tacrolimus and ascomycin, m/z 826.5 and 814.5, respectively. Collision-induced dissociation (CID) fragment ions of tacrolimus and ascomycin (m/z 616.4 and 604.4, respectively) are also monitored to enhance overall selectivity. Total run time is 1 minute per injection. A plot of chromatographic peak height ratio of m/z 826.5 to m/z 814.5 vs tacrolimus blood concentration is linear from the lowest limit of quantitation (0.3 microg/L) to at least 120.0 microg/L. Metabolites of tacrolimus and other immunosuppressive and commonly prescribed drugs do not interfere. Analytical recovery is 94% to 113% over a range of 4.4 to 41.0 microg/L. Between-run precision coefficients of variation (CV) are 3.6% to 4.2% over a range of 8.1 to 32.4 microg/L. Comparison data (n = 156) of the LC/MS method versus a commercial immunoassay (Abbott Tacrolimus IMx MEIA II) (Abbott; Chicago, IL) demonstrated that the tacrolimus concentration as measured by LC/MS = (0.912 x MEIA concentration) - 0.049; r2 = 0.968. This validated LC/MS method demonstrates significant advantages over conventional immunoassays and improves on the lower limit of detection, sensitivity, accuracy, and range of linearity. In the authors' laboratory, this method is approximately 60% less costly to perform than the most commonly implemented immunoassay. Together, the authors' daily clinical experience during 1 year and participation in a proficiency testing program has demonstrated that the method is robust and suitable for routine therapeutic monitoring of tacrolimus.
Open access and beyond (2006)

Uncensored exchange of scientific results hastens progress. Open Access does not stop at the removal of price and permission barriers; still, censorship and reading disabilities, to name a few, hamper access to information. Here, we invite the scientific community and the public to discuss new methods to distribute, store and manage literature in order to achieve unfettered access to literature.
Intrathecal neuropeptide Y reduces behavioral and molecular markers of inflammatory or neuropathic pain (2007)

Our previous work indicates that the intrathecal administration of neuropeptide Y (NPY) acts at its cognate receptors to reduce behavioral signs of nociception in several models of inflammatory pain, including the formalin test. The present study extends these findings to a rat model of peripheral neuropathic pain, and then evaluates the hypothesis that NPY inhibits inflammation- and nerve injury-induced activation of spinal nociceptive transmission. Here we show that NPY dose-dependently reduced behavioral signs of mechanical and cold hypersensitivity in the spared nerve injury (SNI) model. Intrathecal administration of either a Y1 (BIBO3304) or a Y2 (BIIE0246) receptor antagonist dose-dependently reversed the anti-allodynic actions of NPY. To monitor the effects of NPY on the stimulus-induced activation of spinal nociresponsive neurons, we quantified protein expression of the immediate-early gene c-fos in lamina I-VI of the L4-L5 dorsal horn, with special attention to the mediolateral pattern of Fos immunohistochemical staining after SNI. Either tactile stimulation of the hindpaw ipsilateral to nerve injury, or intraplantar injection of noxious formalin, increased the number of Fos-like immunoreactive profiles. Tactile stimulation evoked a mediolateral pattern of Fos expression corresponding to the innervation territory of the uninjured (sural) nerve. We found that intrathecal NPY reduced both formalin- and SNI-induced Fos expression. NPY inhibition of SNI-induced Fos expression was localized to the sural (uninjured) innervation territory, and could be blocked by intrathecal BIBO3304 and BIIE0246. We conclude that NPY acts at spinal Y1 and Y2 receptors to reduce spinal neuron activity and behavioral signs of inflammatory or neuropathic pain.
HMG-CoA reductase inhibition induces IL-1β release through Rac1/PI3K/PKB-dependent caspase-1 activation (2008)

Mevalonate kinase deficiency (MKD) is an autoinflammatory disorder characterized by recurring fever episodes and results from disturbed isoprenoid biosynthesis. Lipopolysaccharide-stimulated peripheral blood mononuclear cells from MKD patients secrete high levels of interleukin-1beta (IL-1beta) because of the presence of hyperactive caspase-1, and this has been proposed to be the primary cause of recurring inflammation. Here we show that inhibition of HMG-CoA reductase by simvastatin treatment, mimicking MKD, results in increased IL-1beta secretion in a Rac1/PI3K-dependent manner. Simvastatin treatment was found to activate protein kinase B (PKB)/c-akt, a primary effector of PI3K, and ectopic expression of constitutively active PKB was sufficient to induce IL-1beta release. The small GTPase Rac1 was activated by simvastatin, and this was required for both PKB activation and IL-1beta secretion. IL-1beta release is mediated by caspase-1, and simvastatin treatment resulted in increased caspase-1 activity in a Rac1/PI3K-dependent manner. These data suggest that, in MKD, dysregulated isoprenoid biosynthesis activates Rac1/PI3K/PKB, resulting in caspase-1 activation with increased IL-1beta release. Importantly, inhibition of Rac1 in peripheral blood mononuclear cells isolated from MKD patients resulted in a dramatic reduction in IL-1beta release. These data suggest that pharmacologic inhibition of Rac1 could provide a novel therapeutic strategy for treatment of MKD.
Banded Iron Formation-Related High-Grade Iron Ore (2008)

Abstract The spark to put together this volume on banded iron formation (BIF)-related high-grade iron ore was born in 2005 during a steamy night in Carajás where the iron research group from the Universidade Federal Minas Gerais, Vale geologists, Carlos Rosière and Steffen Hagemann, were hotly debating the hypogene alteration genesis for the high-grade, jaspilite-hosted Serra Norte iron ore deposits. A couple of caipirinhas later we decided that the time was opportune to put together a volume that captured the new and innovative research that was being conducted on BIF-related high-grade iron ores throughout the world. We had little problem convincing our South African colleagues Jens Gutzmer and Nic Beukes to join the effort and decided that the 2008 biannual Society of Economic Geologists' (SEG) meeting in South Africa would be the perfect place to present this project through a combined field trip and workshop near Sishen. The enthusiastic support that we received from the research community, SEG, and industry to put this volume together was generated by the significant increase in exploration activity, and with it the need for more detailed information on what exactly controls the location of high-grade iron orebodies, and renewed research interest around the world in models for the genesis of BIF-related high-grade iron ore, and particularly the relative importance of hypogene and supergene processes in formation of high-grade ore. This volume concentrates on new research on the characteristics and metallogenesis of BIF-related high-grade iron ores. It contains a state of the art series of papers on established and new iron ore districts and deposits, the different components of the BIF iron mineral system, and how to best explore for this ore type. Although the emphasis of many of the contributions to this volume is on the hypogene aspect of high-grade iron ore formation, it is important to note that most BIF-related iron ore districts have a very pronounced supergene overprint due to deep lateritic weathering. The transformation of many hypogene iron orebodies of reasonable grade and size to the giant deposits exploited today can be related to this geologically recent supergene overprint; most of the past and still much of the present mining of high-grade iron ore relates to soft ore interpreted in most cases to be the direct result of supergene processes. Also mentioned here should be the recent resurgence of a syngenetic model that advocates the formation of chert-free BIF
Signal Quality Evaluation Method for Over-30-GB Blu-ray Discs (2006)

We proposed a new signal quality evaluation method applying a sequenced amplitude margin (SAM) for over 30-GB Blu-ray discs (BDs), where the dominant error pattern differs from lower-density discs (23–27-GB BDs), and conventional signal quality evaluation method such as maximum-likelihood sequence error (MLSE) lose accuracy. We experimentally confirmed the effectiveness of our new method for 30–31.5-GB BDs.
Mean Temperature Difference and Heat Transfer Coefficient in Liquid Heat Exchangers (1933)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMean Temperature Difference and Heat Transfer Coefficient in Liquid Heat ExchangersAllam P. Colburn and E. I. du Pont deCite this: Ind. Eng. Chem. 1933, 25, 8, 873–877Publication Date (Print):August 1, 1933Publication History Published online1 May 2002Published inissue 1 August 1933https://pubs.acs.org/doi/10.1021/ie50284a010https://doi.org/10.1021/ie50284a010research-articleACS PublicationsRequest reuse permissionsArticle Views1339Altmetric-Citations49LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Expression of transforming growth factor‐alpha in regenerating liver and during hepatic differentiation (1992)

Both the level of expression and cellular distribution of transcripts for transforming growth factor-alpha (TGF-alpha) were studied in adult rat liver after partial hepatectomy and during hepatic differentiation in fetal, neonatal, and adult livers by northern blot analysis and in situ hybridization. A marked increase in the expression of TGF-alpha was observed in neonatal livers and in adult livers after partial hepatectomy and during hepatic regeneration following modification of the Solt-Farber protocol. Quantitation of silver grains after in situ hybridization with a TGF-alpha riboprobe revealed a sixfold to eightfold increase in fetal and neonatal hepatocytes. Moreover, the expression of TGF-alpha in the liver 3 wk after birth was still fourfold higher than that of the adult quiescent liver. Both proliferating oval cells and basophilic foci of hepatocytes generated by modification of the Solt-Farber protocol were positive for TGF-alpha transcripts. The level of TGF-alpha transcripts was sixfold higher in the basophilic foci than in the surrounding liver. High concentrations of TGF-alpha transcripts were observed in the oval cells that lined pseudoducts and in the transitional cells proliferating within the ducts. The combination of in situ hybridization and immunocytochemistry using cell-specific antibodies revealed the presence of TGF-alpha transcripts in both oval cells and in perisinusoidal stellate cells. The observation that TGF-alpha transcripts were found both in primitive liver epithelial cells and perisinusoidal stellate cells suggests that this growth factor, in addition to its mitogenic action, may also have other important functions in the liver.
Towards emotion prediction in spoken tutoring dialogues (2003)

Human tutors detect and respond to student emotional states, but current machine tutors do not. Our preliminary machine learning experiments involving transcription, emotion annotation and automatic feature extraction from our human-human spoken tutoring corpus indicate that the spoken tutoring system we are developing can be enhanced to automatically predict and adapt to student emotional states.
Human Action Recognition Using Tensor Principal Component Analysis (2012)

Abstract—Human action can be naturally represented as multidimensional arrays known as tensors. In this paper, a simple and efficient algorithm based on tensor subspace learning is proposed for human action recognition. An action is represented as a 3th-order tensor first, then tensor principal component analysis is used to reduce dimensionality and extract the most useful features for action recognition. So the spatial and temporal correlations of the action are preserved. After then, a nearest neighbor classifier based on tensor distance is used to recognize action, in other words, measuring the similarity between actions using tensor distance in tensor subspace. The proposed method is assessed by using a public video database, namely Weizmann human action data sets. Experimental results reveal that the proposed method performs very well on that data sets, and robustness test has been carried out to testify the effectiveness. Keywords-Tensor Principal Component；Tensor Distance； Human Action Recognition ； Tensor Subspace learning
An optimal selection of regression variables (1981)

An asymptotically optimal selection of regression variables is proposed. The key assumption is that the number of control variables is infinite or increases with the sample size. It is also shown that Mallows's Cp', Akaike's FPE and aic methods are all asymptotically equivalent to this method.