gebhart/patrepeval huggingface dataset:
[Expression of Chemokine receptor CXCR6 and its significance in breast cancer cell lines]. (2014)

Different invasive breast cancer cell lines express CXCR6 at different levels, positively correlated with its invasive ability.
Tuning Biosensor Cross-Reactivity Using Aptamer Mixtures (2020)

It is challenging to tune the response of biosensors to a set of ligands, for example, cross-reactivity to a given target family while maintaining high specificity against interferents, due to the lack of suitable bioreceptors. We present a novel approach for controlling the cross-reactivity of biosensors by employing defined mixtures of aptamers that have differing binding properties. As a demonstration, we develop assays for the specific detection of a family of illicit designer drugs, the synthetic cathinones, with customized responses to each target ligand and interferent. We first use a colorimetric dye-displacement assay to show that the binding spectra of dual-aptamer mixtures can be tuned by altering the molar ratio of these bioreceptors. Optimized assays achieve broad detection of synthetic cathinones with minimal response toward interferents and generally demonstrate better sensing performance than assays utilizing either aptamer alone. The generality of this strategy is demonstrated with a dual-aptamer electrochemical sensor. Our approach enables customization of biosensor responsiveness to an extent that has yet to be achieved through any previously reported aptamer engineering techniques such as sequence mutation or truncation. Since multiple aptamers for the designated target family can routinely be identified via high-throughput sequencing, we believe our strategy offers a generally applicable method for generating near-ideal aptamer biosensors for various analytical applications, including medical diagnostics, environmental monitoring, and drug detection.
Male and Female Genomes Associated in a Single Pronucleus in Human Zygotes (1995)

The ploidy of single-pronucleated human zygotes obtained after conventional in vitro fertilization was determined by fluorescent in situ hybridization (FISH) using multiple simultaneous probes for gonosomes and autosomes. After zona removal the single-pronucleated zygotes were exposed to cytochalasin B, and the pronucleus, surrounded by scant cytoplasm and the plasma membrane (karyoplast), was divided from the rest of the egg (cytoplast). The karyoplasts and the corresponding cytoplasts were analyzed separately by FISH. Of the 16 zygotes analyzed, 10 had haploid pronuclei and 6 were diploid. Four diploid pronuclei contained XY chromosomes, and 2 contained XX chromosomes. These results suggest that during the course of their interaction, human gamete nuclei can associate together and form diploid, single-pronucleated zygotes. These findings confirm a newly recognized variation of human pronuclear interaction during syngamy.
Altered Activation of Protein Kinase PKR and Enhanced Apoptosis in Dystonia Cells Carrying a Mutation in PKR Activator Protein PACT (2015)

PACT is a stress-modulated activator of the interferon-induced double-stranded RNA-activated protein kinase (PKR). Stress-induced phosphorylation of PACT is essential for PACT's association with PKR leading to PKR activation. PKR activation leads to phosphorylation of translation initiation factor eIF2α inhibition of protein synthesis and apoptosis. A recessively inherited form of early-onset dystonia DYT16 has been recently identified to arise due to a homozygous missense mutation P222L in PACT. To examine if the mutant P222L protein alters the stress-response pathway, we examined the ability of mutant P222L to interact with and activate PKR. Our results indicate that the substitution mutant P222L activates PKR more robustly and for longer duration albeit with slower kinetics in response to the endoplasmic reticulum stress. In addition, the affinity of PACT-PACT and PACT-PKR interactions is enhanced in dystonia patient lymphoblasts, thereby leading to intensified PKR activation and enhanced cellular death. P222L mutation also changes the affinity of PACT-TRBP interaction after cellular stress, thereby offering a mechanism for the delayed PKR activation in response to stress. Our results demonstrate the impact of a dystonia-causing substitution mutation on stress-induced cellular apoptosis.
Intranasal vaccination of recombinant H5N1 HA1 proteins fused with foldon and Fc induces strong mucosal immune responses with neutralizing activity: Implication for developing novel mucosal influenza vaccines (2015)

The highly pathogenic avian influenza (HPAI) H5N1 virus remains a threat to public health because of its continued spread in poultry in some countries and its ability to infect humans with high mortality rate, calling for the development of effective and safe vaccines against H5N1 infection. Here, we constructed 4 candidate vaccines by fusing H5N1 hemagglutinin 1 (HA1) with foldon (HA1-Fd), human IgG Fc (HA1-Fc), foldon and Fc (HA1-FdFc) or His-tag (HA1-His). We then compared their ability to induce mucosal immune responses and neutralizing antibodies in the presence or absence of Poly(I:C) and CpG adjuvants via the intranasal route. Without an adjuvant, HA1-FdFc could elicit appreciable humoral immune responses and local mucosal IgA antibodies in immunized mice, while other vaccine candidates only induced background immune responses. In the presence of Poly(I:C) and CpG, both HA1-Fd and HA1-Fc elicited much higher levels of serum IgG and local mucosal IgA antibodies than HA1-His. Poly(I:C) and CpG could also augment the neutralizing antibody responses induced by these 4 vaccine candidates in the order of HA1-FdFc > HA1-Fc > HA1-Fd > HA1-His. These results suggest that both Fd and Fc potentiate the immunogenicity of the recombinant HA1 protein and that Poly(I:C) and CpG serve as efficient mucosal adjuvants in promoting efficacy of these vaccine candidates to induce strong systemic and local antibody responses and potent neutralizing antibodies, providing a useful strategy to develop effective and safe mucosal H5N1 vaccines.
<i>Saccharomyces cerevisiae</i> -Based Molecular Tool Kit for Manipulation of Genes from Gram-Negative Bacteria (2006)

A tool kit of vectors was designed to manipulate and express genes from a wide range of gram-negative species by using in vivo recombination. Saccharomyces cerevisiae can use its native recombination proteins to combine several amplicons in a single transformation step with high efficiency. We show that this technology is particularly useful for vector design. Shuttle, suicide, and expression vectors useful in a diverse group of bacteria are described and utilized. This report describes the use of these vectors to mutate clpX and clpP of the opportunistic pathogen Pseudomonas aeruginosa and to explore their roles in biofilm formation and surface motility. Complementation of the rhamnolipid biosynthetic gene rhlB is also described. Expression vectors are used for controlled expression of genes in two pseudomonad species. To demonstrate the facility of building complicated constructs with this technique, the recombination of four PCR-generated amplicons in a single step at >80% efficiency into one of these vectors is shown. These tools can be used for genetic studies of pseudomonads and many other gram-negative bacteria.
Axial mode selectivity in active distributed-reflector for dynamic-single-mode lasers (1985)

The theory for design of active distributed-reflector lasers is discussed from the point of view of dynamic-single-mode (DSM) characteristics. Optimum conditions and fabrication tolerances of coupling coefficient, cavity length, length and location of phase-shifted region, and the effect of the reflection at the end mirrors are discussed. Asymmetric active distributed-reflector laser with different coupling coefficients and lengths of two corrugation regions is also proposed to improve the efficiency of the front facet output.
Effect of the clay mineral zeolite on ammonia inhibition of anaerobic thermophilic reactors treating cattle manure (1996)

Abstract Addition of zeolite counteracted to some extent the inhibitory effect of ammonia during thermophilic anaerobic digestion of cattle manure. In continuously‐fed reactor experiments, addition of zeolite delayed the onset of the inhibition and aided process recovery after initial inhibition. The effect was observed mainly when the ammonia concentration was increased gradually, indicating that the major effect of zeolite was not through a direct antagonistic effect towards ammonia but through an increased process resistance to toxic compounds. In batch experiments zeolite had a similar stimulatory effect leading to a decreased lag phase and increased methane production rate in ammonia inhibited reactors. Key words: Anaerobic digestionCattle manureZeoliteAmmonia inhibitionThermophilic reactors.
(2E)-N,N-dibutyl-3-(4-hydroxy-3-methoxyphenyl)acrylamide induces apoptosis and cell cycle arrest in HL-60 cells. (2007)

These results enhance our understanding of the mechanisms of action of compound 8-mediated anticancer effects.
Identification and Characterization of UDP-GalNAc: NeuAcα2–3Galβ1–4Glc(NAc) β1–4(GalNAc to Gal)N-Acetylgalacto saminyltransferase in Human Blood Plasma1 (1987)

Normal human plasma was found to contain beta 1-4N-acetylgalactosaminyltransferase catalyzing the transfer of N-acetylgalactosamine from UDP-GalNAc to 3'-sialyl-lactose, NeuAc alpha 2-3Gal beta 1-4Glc. The transferred N-acetylgalactosaminyl residue was cleaved from the desialylated reaction product by the beta-N-acetylhexosaminidase from jack beans. Methylation and hydrolysis of the desialylated reaction product yielded only 2,3,6-tri-O-methylgalactose and 2,3,6-tri-O-methylglucose as neutral sugars, indicating that the N-acetylgalactosaminyl residue was introduced at position C-4 of the galactosyl residue of 3'-sialyllactose. The enzyme required Mn2+ ions for its activity and showed a pH optimum between 6.5 and 8.5. By using a wide variety of oligosaccharides and glycoconjugates, the acceptor specificity of the beta 1-4N-acetylgalactosaminyltransferase was investigated. No detectable amount of N-acetylgalactosamine was transferred to either 6'-sialyllactose or lactose. The enzyme did not act on ganglioside GM3, NeuAc alpha 2-3Gal beta 1-4Glc-ceramide, suggesting that the hydrophobic ceramide portion of GM3 interferes with the enzyme reaction. On the other hand, glycoproteins carrying terminal NeuAc alpha 2-3Gal beta 1-4GlcNAc structures on their N-linked oligosaccharide chains, e.g. Tamm-Horsfall glycoprotein, were efficient acceptors.
Peroxyoxalate chemiluminescence detection of polycyclic aromatic amines in liquid chromatography (1984)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPeroxyoxalate chemiluminescence detection of polycyclic aromatic amines in liquid chromatographyKenneth W. Sigvardson, John M. Kennish, and John W. BirksCite this: Anal. Chem. 1984, 56, 7, 1096–1102Publication Date (Print):June 1, 1984Publication History Published online1 May 2002Published inissue 1 June 1984https://pubs.acs.org/doi/10.1021/ac00271a011https://doi.org/10.1021/ac00271a011research-articleACS PublicationsRequest reuse permissionsArticle Views292Altmetric-Citations118LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Characteristics of motion artifacts in cardiac optical mapping studies (2009)

Motion artifacts are one of the issues in cardiac optical mapping studies. This paper is focused on the description of the motion artifacts caused by planar movement. The theory of its origin and possibilities of its suppression is described. The suppression of the motion artifacts is based on image registration techniques. There are introduced the characteristics about influence of the weighted area averages on presence of these artifacts. In this study conventional pharmacological or mechanical ways of motion artifacts suppression were not involved.
High current stress effects in amorphous-InGaZnO4 thin-film transistors (2013)

Amorphous-InGaZnO4 (a-IGZO) thin-film transistors (TFTs) on glass undergo large positive threshold voltage shifts (ΔVTH) under high current stress (HCS)—a consequence of Joule heating of the active-layer. Here, we show that when the active layer is split into smaller parts, HCS induces negligible ΔVTH. When the active layer heats up during HCS, conducting electrons in the channel gain enough energy to surmount the energy barrier at the active-layer/gate-insulator interface and become trapped into deep states inside the gate-insulator. Splitting the active-layer into smaller parts increases the surface area for heat dissipation, resulting in the elimination of these self-heating effects.
FREQUENT ANTIBIOTIC THERAPY IMPROVES SURVIVAL OF CYSTIC FIBROSIS PATIENTS WITH CHRONIC <i>PSEUDOMONAS AERUGINOSA</i> INFECTION (1983)

During the period 1971-75, 51 cystic fibrosis (CF) patients who contracted chronic P. aeruginosa infection were treated at the Danish CF centre with anti-pseudomonas chemotherapy only when their clinical condition deteriorated considerably. During the period 1976-80, 58 CF patients who contracted chronic P. aeruginosa infection were treated at the Danish CF centre with anti-pseudomonas chemotherapy on a regular basis every 3 months. Each routine 24 day-course of chemotherapy consisted of tobramycin in combination with carbenicillin or other beta-lactam antibiotics with activity against P. aeruginosa. In case of allergy or resistant strains monotherapy with tobramycin was used. The 5-year survival of CF patients from the time of the onset of the chronic P. aeruginosa infection increased from 54% in the first period to 82% in the second period (p less than 0.05), and lung function (peak expiratory flow rate) also improved significantly. It is concluded that intensive "maintenance" chemotherapy against P. aeruginosa improves survival and quality of life of CF patients although permanent eradication of P. aeruginosa is not accomplished.
Anomalous dependence of threshold current on stripe width in gain-guided strained-layer InGaAs/GaAs quantum well lasers (1989)

An anomalous dependence of the threshold current on the stripe width is observed for gain-guided strained-layer InGaAs/GaAs quantum well lasers. The threshold current increases strongly as the stripe width is reduced from relatively large values. This is attributed to the huge lateral loss caused by an unusually large index antiguide which manifests itself in the far-field behavior. This large loss also leads to a population of higher quantized energy levels in the InGaAs quantum well strongly reducing the lasing wavelength by as much as 61 nm.
Reference-free objective quality metrics for MPEG-coded video (2005)

With the growth of digital video delivery, there is an increasing demand for better and more efficient ways to measure video quality. Most existing video quality metrics are reference-based approaches that are not suitable to measure the video quality perceived by the end user without access to reference videos. In this paper, we propose a reference-free video quality metric for MPEG coded videos. It predicts subjective quality ratings using both reference-free MPEG artifact measures and MPEG system parameters (known or estimated). The advantage of this approach is that it does not need a precise separation of content and artifact or the removal of any artifacts. By exploring the correlations among different artifacts and system parameters, our approach can remove content dependency and achieve an accurate estimate of the subjective ratings.
Scaling of Multimillion-Atom Biological Molecular Dynamics Simulation on a Petascale Supercomputer (2009)

A strategy is described for a fast all-atom molecular dynamics simulation of multimillion-atom biological systems on massively parallel supercomputers. The strategy is developed using benchmark systems of particular interest to bioenergy research, comprising models of cellulose and lignocellulosic biomass in an aqueous solution. The approach involves using the reaction field (RF) method for the computation of long-range electrostatic interactions, which permits efficient scaling on many thousands of cores. Although the range of applicability of the RF method for biomolecular systems remains to be demonstrated, for the benchmark systems the use of the RF produces molecular dipole moments, Kirkwood G factors, other structural properties, and mean-square fluctuations in excellent agreement with those obtained with the commonly used Particle Mesh Ewald method. With RF, three million- and five million-atom biological systems scale well up to ∼30k cores, producing ∼30 ns/day. Atomistic simulations of very large systems for time scales approaching the microsecond would, therefore, appear now to be within reach.
CO Oxidation on Positively and Negatively Charged Ag<sub>13</sub>Nanoparticles (2011)

Utilizing density functional theory calculations and a modified kinetic model, we report the CO oxidation reactivity of negatively and positively charged isolated cuboctahedron (COh) and icosahedron (Ih) Ag13 nanoparticles. Charging the nanoparticles modifies the electron distribution in the core and shell atoms as well as the structural stability of the Ag nanoparticles. During the reaction, Ih Ag13 nanoparticles can be easily deformed into an amorphous or COh structure, which is more stable than the Ih structure. However, it does not function well as a renewable catalyst. Although a neutral COh Ag13 nanoparticle exhibits relatively poor reactivity, the reactivity is enhanced significantly by excess electrons. This study provides fundamental insight into how the electronic and structural interaction between an oxide support and the supported nanoparticle affects the catalytic activity of the general nanocatalyst.
ASM engineering materials reference book (1994)

Better than ever! The all-in-one reference book on physical and mechanical properties, processing parameters, and design and machining data of engineered materials Heavily revised: includes greatly expanded Ceramics coverage and two new sections on Traditional Ceramics and Glasses Engineering Plastics coverage includes a new section on Structure and Properties and expanded information on Production and Machining Electronic Material coverage has been reorganized for faster reference Alloy phase diagrams have been omitted to provide more of the information requested nu users. This popular reference contains four major areas on Composites, Ceramics, Engineering Plastic and Electronic materials. Each area compiles and organizes a tabular information and molecular structures where applicable. A complete table of contents guides you quickly to the information for which you are looking. The greatly expanded Ceramics area contains 61 tables of information for the following categories: standards and general applications thermodynamic and solid state data physical and mechanical properties high temperature applications friction, wear and abrasion environmenta effects manufacturing and production. A new section, Traditional Ceramics, contains 48 tables covering the areas of whitwares and clay-based products tile and brick cements and concretes refractories glazes, enamels and coatings processing. A second new section on Glasses contains 50 tables for the areas of composition and applications (for various categories of glasses), properties, glass-ceramics and glass-metal seals. In the Plastics area, the section on Thermoplastics contains 167 tables and 23 figures, as well as a new section on Structures and properties. Coverage of production and Machining has been expanded to include moulding and forming processes, processing parameters for specific materials, and secondary operations (including machining).
Computer-aided Diagnosis System on Breast Ultrasound (2007)

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Representation Flow for Action Recognition (2019)

In this paper, we propose a convolutional layer inspired by optical flow algorithms to learn motion representations. Our representation flow layer is a fully-differentiable layer designed to capture the `flow' of any representation channel within a convolutional neural network for action recognition. Its parameters for iterative flow optimization are learned in an end-to-end fashion together with the other CNN model parameters, maximizing the action recognition performance. Furthermore, we newly introduce the concept of learning `flow of flow' representations by stacking multiple representation flow layers. We conducted extensive experimental evaluations, confirming its advantages over previous recognition models using traditional optical flows in both computational speed and performance. The code is publicly available.
Simulation of Transverse Modes in Vertical-Cavity Surface-Emitting Lasers (1998)

An approximation to the Maxwell-Semiconductor Bloch equations is solved for the transverse modes of vertical-cavity surface-emitting lasers. A finite-difference algorithm will be used to calculate the detailed time evolution of the transverse mode profiles of VCSELs. Effects of pumping current and refractive index profiles will be investigated.
Aqueous <i>vs.</i> nonaqueous Zn-ion batteries: consequences of the desolvation penalty at the interface (2018)

Comparison of intercalation of Zn<sup>2+</sup> in layered V<sub>3</sub>O<sub>7</sub>·H<sub>2</sub>O in non-aqueous and aqueous electrolytes reveals a much higher desolvation penalty at the non-aqueous interface, a major factor in dictating the kinetics.
Preparation and Characterization of Polyaniline Nanotubes Doped with Different Acid (2013)

Polyaniline (PANI) nanotubes were synthesized separately using amino acetic acid (AA), ethylenediamine tetraacetic acid (EDTA), oxalic acid (OA) as dopant and ammonium persulfate (APS) as oxidant by a self-assembly method. SEM, TEM,FTIR and X-ray diffraction (XRD) and applying the 4 probes method characterized the morphology, structure and property of the product. It was found that nanotubes morphology were synthesized when the [Aci/[A ratio is 1:2.The room template conductivity of the products were studied.
Catalytic oxidation of methanol by 12-molybdosilicic acid supported on silica: dispersion effect (1988)

J. Tatibouët, M. Che, M. Amirouche, M. Fournier and C. Rocchiccioli-Deltcheff, J. Chem. Soc., Chem. Commun., 1988, 1260 DOI: 10.1039/C39880001260
Activation of CD4 cells by fibronectin and anti-CD3 antibody. A synergistic effect mediated by the VLA-5 fibronectin receptor complex. (1989)

In this study, fibronectin synergized with anti-CD3 antibody to promote CD4 cell proliferation in a serum-free culture system. The cell-adhesive domain plus additional regions of the fibronectin molecule are involved in this synergy. Anti4B4(CDw29) antibody blocked the activation of CD4 cells in this system. Furthermore, it is the VLA-5 protein within the set of molecules recognized by anti-4B4 that serves as a fibronectin receptor on the CD4 lymphocytes. The VLA-5 fibronectin receptor was mainly expressed on CD4+ CD45R-CDw29+ cells and may in part contribute to the unique function of these cells.