gebhart/patrepeval huggingface dataset:
Establishment of <scp><i>TSH </i></scp>β real‐time monitoring system in mammalian photoperiodism (2013)

Organisms have seasonal physiological changes in response to day length. Long-day stimulation induces thyroid-stimulating hormone beta subunit (TSHβ) in the pars tuberalis (PT), which mediates photoperiodic reactions like day-length measurement and physiological adaptation. However, the mechanism of TSHβ induction for day-length measurement is largely unknown. To screen candidate upstream molecules of TSHβ, which convey light information to the PT, we generated Luciferase knock-in mice, which quantitatively report the dynamics of TSHβ expression. We cultured brain slices containing the PT region from adult and neonatal mice and measured the bioluminescence activities from each slice over several days. A decrease in the bioluminescence activities was observed after melatonin treatment in adult and neonatal slices. These observations indicate that the experimental system possesses responsiveness of the TSHβ expression to melatonin. Thus, we concluded that our experimental system monitors TSHβ expression dynamics in response to external stimuli.
Tasco-Forage: I. Influence of a seaweed extract on antioxidant activity in tall fescue and in ruminants. (2001)

Seaweed (Ascophyllum nodosum) is a known source of plant growth regulators, and application to turfgrasses has increased activity of the antioxidant superoxide dismutase (SOD) and specific vitamin precursors. Increased antioxidant activity in both plants and animals diminishes oxidative stress. Two pasture experiments investigated effects of Tasco-Forage (a proprietary seaweed-based product) applied to tall fescue (Festuca arundinacea) on antioxidant activity in plants and in ruminants that grazed the forage. In Exp. 1, fescue was 70 to 100% infected with the endophyte fungus Neotyphodium coenophialum ([Morgan-Jones and Gams] Glenn, Bacon, and Hanlin). Twenty-four wether lambs (initial BW 41 kg; SD = 5) grazed fescue treated with 0, 1.7, or 3.4 kg Tasco/ha applied in April and July, 1994, with four replications per treatment. Grazing occurred for 26 d beginning April 21 and for 22 d beginning July 19. In July, there was a linear increase in daily gains (P < 0.05), and serum vitamin A (P < 0.13) and whole-blood Se (P < 0.10) tended to increase in lambs grazing Tasco-treated fescue. In Exp. 2,48 Angus and Angus x Hereford steers (initial BW 245 kg; SD = 20) grazed infected or uninfected tall fescue in Virginia that was treated (3.4 kg/ ha) or untreated with Tasco in April and July, 1995. Steers that grazed infected tall fescue had lower (P < 0.02) serum vitamin A and E and tended (P < 0.07) to have lower whole-blood Se in September compared with steers that grazed uninfected tall fescue. Tasco decreased (P < 0.06) serum vitamin E but tended to increase whole-blood Se (P < 0.10) in September and serum vitamin A in July (P < 0.12). During 1996 and 1997, the experiment was repeated in Virginia with Angus steers and was replicated at Prairie, MS, where 1/4 Brahman x 3/4 Angus steers were used. Forty-eight steers were included at each location in each year (n = 192 total steers for 1996 and 1997). Steers that grazed infected tall fescue in Mississippi had lower (P < 0.05) serum vitamin E by the end of the grazing season. At both locations Tasco increased (P < 0.05) activity of superoxide dismutase in both infected and uninfected fescue. The endophyte in tall fescue seemed to decrease antioxidant activity in grazing steers, whereas Tasco seemed to increase antioxidant activity in both the forage and the grazing ruminant. Tasco may provide opportunities to reduce oxidative stress in plants and animals.
Superresolved spatially multiplexed interferometric microscopy (2017)

Superresolution capability by angular and time multiplexing is implemented onto a regular microscope. The technique, named superresolved spatially multiplexed interferometric microscopy (S2MIM), follows our previously reported SMIM technique [Opt. Express22, 14929 (2014)OPEXFF1094-408710.1364/OE.22.014929, J. Biomed. Opt.21, 106007 (2016)JBOPFO1083-366810.1117/1.JBO.21.10.106007] improved with superresolved imaging. All together, S2MIM updates a commercially available non-holographic microscope into a superresolved holographic one. Validation is presented for an Olympus BX-60 upright microscope with resolution test targets.
Poultry at high altitudes: Chicken colony at White Mountain Research Station is used for study of the adaptation of animals to climatic stress (1959)

Physiological adaptation–the ability to change so as to live more or less normally in a previously unfavorable or stressing environment–is one of the most universal properties of living things. However, the native ability of animals to adjust to variations in their environment has to a large extent been sacrificed in breeding animals for modern high standards of production.
Xerostomia and hyposalivation: a preliminary report of their prevalence and associated factors in Brazilian elderly diabetic patients. (2010)

The prevalence of dry mouth and hyposalivation was high in elderly diabetics. Only at-home jobs were observed to be associated with dry mouth. There was no association between hyposalivation and the variables assessed, with regard to either unstimulated or stimulated saliva. Further studies, involving larger samples, are required to confirm the present findings.
Optimizing sort order query execution in balanced and nested grid files (1995)

Disk input/output (I/O) efficient query execution is an important topic with respect to DBMS performance. In this context, we elaborate on the construction of disk access plans for sort order queries in balanced and nested grid files. The key idea is to use the order information contained in the directory of the multiattribute search structure. The presented algorithms are shown to yield a significant decrease in the number of disk I/O operations by appropriate use of the order information. Two algorithms for the construction of appropriate disk access plans are proposed, namely a greedy approach and a heuristic divide-and-conquer approach. Both approaches yield considerable I/O savings compared to straightforward query processing without consideration of any directory order information. The former performs well for small buffer page allocations, i.e., for a small number of buffer pages relative to the number of data buckets processed in the query. The latter is superior to the greedy algorithm with respect to the total number of I/O operations and with respect to the overall maximum of buffer pages needed to achieve the minimal number of disk I/O operations. Both approaches rely on a binary trie as a temporary data structure. This trie is used as an explicit representation of the order information. The storage consumption of the temporary data structure is shown to be negligible in realistic cases, Even for pathological cases with respect to degenerated balanced and nested grid files, reasonable upper bounds can be given.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Oxygen adsorption on Ag powder (1989)

Oxygen adsorption on a Ag powder sample is different from that on a supported Ag catalyst. The powder shows the presence of a very high temperature desorption state (at 900 K) which diminishes adsorption into the state which is common to all types of silver samples (desorption peak at 560 K). This behaviour, together with the increased sticking probability which is observed on the powder, indicates that the powder has a very much rougher surface structure than a catalyst, which consists of mostly (111) planes. The powder probably consists of a high proportion of (111) planes with a high density of steps and defects since the high-temperature desorption state has been seen on a stepped single-crystal plane [Ag(331)].
The ligand for c-kit, stem cell factor, stimulates the circulation of cells that engraft lethally irradiated baboons (1992)

Recombinant human stem cell factor (SCF), the ligand for c-kit, has been shown to stimulate increased numbers of hematopoietic progenitor cells of multiple types to circulate in the blood of baboons, but it was not known if the cells stimulated to circulate by SCF contained cells capable of engrafting and rescuing lethally irradiated baboons. Peripheral blood mononuclear cells (PBMNC) were collected by leukapheresis from four untreated control baboons and from three baboons on the 10th or 11th day of treatment with SCF (200 micrograms/kg/d). All animals were transplanted with 1.00 to 1.04 x 10(8)/kg of cryopreserved autologous PBMNC after treatment with a single dose of 1,020 cGy total body irradiation (TBI). Three animals were transplanted with PBMNC that had been collected during SCF treatment, 24 to 38 days after the last dose of SCF. Rapid trilineage engraftment was documented by bone marrow biopsy in all three. The mean time to a total white blood cell count (WBC) > or = 500/microL, WBC > or = 1,000/microL, and an absolute neutrophil count (ANC) > or = 500/microL was 15 +/- 3 (mean +/- SD), 19 +/- 1, and 19 +/- 2 days, respectively. Two animals remain alive with stable engraftment more than 180 and 245 days posttransplant. The third died of sepsis 32 days posttransplant with a hypercellular marrow showing trilineage engraftment. The surviving animals were transfusion independent by 10 and 59 days posttransplant. Four control animals were transplanted with PBMNC collected in the absence of SCF stimulation. One was treated for 11 days with SCF (200 micrograms/kg/d) after PBMNC were collected. This animal was transplanted 25 days after the last dose of SCF. None of the four control animals engrafted and they died 13, 16, 28, and 38 days posttransplant with marrow aplasia. Treatment with SCF stimulates the circulation of cells that engraft and rescue lethally irradiated baboons. The characteristics of the transplantable cells present in the circulation are now amenable to direct study.
Tunable Nanoscale Graphene Magnetometers (2009)

The detection of magnetic fields with nanoscale resolution is a fundamental challenge for scanning probe magnetometry, biosensing, and magnetic storage. Current technologies based on giant magnetoresistance and tunneling magnetoresistance are limited at small sizes by thermal magnetic noise and spin-torque instability. These limitations do not affect Hall sensors consisting of high mobility semiconductors or metal thin films, but the loss of magnetic flux throughout the sensor's thickness greatly limits spatial resolution and sensitivity. Here we demonstrate graphene extraordinary magnetoresistance devices that combine the Hall effect and enhanced geometric magnetoresistance, yielding sensitivities rivaling that of state of the art sensors but do so with subnanometer sense layer thickness at the sensor surface. Back-gating provides the ability to control sensor characteristics, which can mitigate both inherent variations in material properties and fabrication-induced device-to-device variability that is unavoidable at the nanoscale.
The role of histones in chromatin remodelling during mammalian spermiogenesis (2004)

One of the most dramatic chromatin remodelling processes takes place during mammalian spermatogenesis. Indeed, during the postmeiotic maturation of male haploid germ cells, or spermiogenesis, histones are replaced by small basic proteins, which in mammals are transition proteins and protamines. However, nothing is known of the mechanisms controlling the process of histone replacement. Two hints from the literature could help to shed light on the underlying molecular events: one is the massive synthesis of histone variants, including testis-specific members, and the second is a stage specific post-translational modification of histones. A new testis-specific 'histone code' can therefore be generated combining both histone variants and histone post-translational modifications. This review will detail these two phenomena and discuss possible functional significance of the global chromatin alterations occurring prior to histone replacement during spermiogenesis.
Nanooxidation of silicon with an atomic force microscope: A pulsed voltage technique (1999)

The use of an atomic force microscope (AFM) as an active tool to realize silicon nanolithography is now well known, using a continuous voltage applied between the AFM tip and the surface. The main drawback of this technique is the poor reliability of the tip due to the strong tip-surface interaction. An original way which both increases the reliability and improves the nanolithography resolution is the use of pulsed voltages instead of continuous polarization. In such a case, the interaction time of the tip with the surface under electric field decreases. The frequency oscillation (in noncontact mode) of the cantilever is taken as a reference, and pulsed voltages with variable phase and duty cycle are used. We show that the variation of the phase allows a 100% modulation of the oxide width. Finally, combining this lithography technique with wet etching, a 17.5 nm wide and 5.5 nm height nanowire has been obtained starting from a silicon-on-insulator substrate.
An efficient fault detection and diagnosis protocolfor vehicular networks (2013)

Over the recent years, with the rapid growth of wireless communication technology, vehicular networks have gained a great deal of attention by the research community. In VANETs, vehicles can communicate with each other or with roadside units that allow access to back-end systems; this can provide drivers with useful information and enable them access to different Intelligent Transportation Systems (ITS) applications. For most these applications, the main goal is to guarantee safety on the road and passenger comfort. Road components are vulnerable to faults such as defected devices or failure in controllers. Therefore, an efficient fault detection and diagnostic model is most needed to mitigate communication problems among VANET components. In this paper, we propose a fault detection and diagnosis protocol for vehicular networks using the comparison based diagnosis approach. We present the protocol and report on its performance evaluation using an extensive set of simulation experiments.
Supramolecular shape memory hydrogels: a new bridge between stimuli-responsive polymers and supramolecular chemistry (2017)

Supramolecular shape memory hydrogels (SSMHs) refer to shape memory polymers, in which temporary shapes are stabilized by reversible crosslinks such as supramolecular interactions and dynamic covalent bonds. Following a brief introduction of the conventional shape memory polymers (SMPs), this tutorial review is focused to summarize the recent advancement in various reversible crosslinks employed to construct SSMHs (supramolecular interactions and dynamic covalent bonds) and different shape memory behaviors (dual and triple shape memory effects). In addition, current challenges and future perspectives in this field are also discussed to suggest a new developing direction.
Spinal neurological complications of achondroplasia (1980)

Spinal neurological complications caused the admission of 17 patients with achondroplasia to the UCLA affiliated hospitals between 1955 and 1979. These patients constituted 41% of all achondroplastic patients admitted during that period. The spinal stenotic syndromes could be divided into three groups: Group I: thoracolumbar stenosis (10 patients); Group II: foramen magnum and upper cervical stenosis (five patients); and Group III: generalized spinal stenosis (two patients). Eleven patients underwent a total of 18 decompressive operative procedures for treatment of paraparesis, quadriparesis, sensory deficits, and sphincter dysfunction. Excellent results were obtained with patients in Group I and II, 77% of whom were ambulatory and continent postoperatively. Two patients in Group III fared less well, showing steady neurological deterioration despite multiple operative procedures. The spectrum of spinal neurological manifestations secondary to achondroplasia is reviewed. Problems with conventional radiological studies and the potential role of computerized tomographic analysis of such patients are discussed. Recommendations for surgical technique are made. Early recognition, prompt clinical evaluation, and safe and accurate radiological analysis of spinal neurological complications of achondroplasia will allow appropriate decompressive surgical procedures to be performed. Excellent results may be anticipated in the reversal and prevention of neurological deficit secondary to achondroplasia with such an approach.
Cardiovascular Applications of Fluorocarbons in Regional Ischemia/Reperfusion (1994)

Potential cardiovascular therapeutic uses for perfluorochemicals include oxygen delivery distal to an occluding balloon during high risk coronary angioplasties, treatment for acute myocardial infarction with or without concomitant reperfusion, cardioplegia, and preservation of donor hearts for transplant. Infusions of oxygenated perfluorochemicals during brief coronary occlusions, as occurs with angioplasties, preserves cardiac ultrastructure and cardiac function. Fluosol is currently approved in the U.S. for angioplasty procedures. Experimental studies have suggested that perfluorochemicals reduce myocardial infarct size during permanent coronary occlusion or temporary coronary occlusion. One school of thought suggests that these agents work by reducing reperfusion injury. By inhibiting neutrophil function, including adherence to endothelial cells and release of toxic substances, perfluorochemicals may preserve the endothelium and prevent no-reflow. However, one might argue that any agent which reduces infarct size by any mechanism would result in less neutrophil infiltration and smaller no-reflow areas. One pilot study suggested that intracoronary Fluosol administered at the time of reperfusion, reduced infarct size and improved regional ventricular function in patients. However, preliminary results of a large multicenter study in which this agent was given along with thrombolysis, were largely negative. Whether perfluorochemicals will become an important adjunctive agent along with reperfusion for acute myocardial infarction remains to be determined.
Performance of Thermal Cements with Different Weighting Materials (2013)

Abstract As the world's demand for energy continues to grow, exploration and production activity increasingly involves operations in high-pressure, high-temperature (HPHT) conditions. HPHT domains include geothermal, steam-injection, and ultradeep wells. These environments introduce difficult technical challenges. A major concern is the set-cement integrity. To maintain zonal isolation throughout the life of an HPHT well, the cement sheath must perform reliably at temperatures that can exceed 315°C [600°F]. When exposed to temperatures higher than 110°C [230°F], set Portland cement undergoes strength retrogression unless additional silica is incorporated in the cement formulation. This phenomenon has been studied extensively. However, less attention has been paid to the effects of other additives on the stability of set Portland cement in an HPHT environment—weighting materials in particular. Weighting materials have largely been assumed to be inert with respect to set Portland cement; however, the present study reveals that this assumption may be false in HPHT environments in which well temperature exceeds 260°C [500°F]. Some weighting agents may react with the set Portland cement, causing strength loss and increased permeability. Fortunately, this effect can be prevented, and the set-cement integrity can be preserved.
Platelet-Derived Mitochondria Display Embryonic Stem Cell Markers and Improve Pancreatic Islet β-cell Function in Humans (2017)

Diabetes is a major global health issue and the number of individuals with type 1 diabetes (T1D) and type 2 diabetes (T2D) increases annually across multiple populations. Research to develop a cure must overcome multiple immune dysfunctions and the shortage of pancreatic islet β cells, but these challenges have proven intractable despite intensive research effort more than the past decades. Stem Cell Educator (SCE) therapy-which uses only autologous blood immune cells that are externally exposed to cord blood stem cells adhering to the SCE device, has previously been proven safe and effective in Chinese and Spanish subjects for the improvement of T1D, T2D, and other autoimmune diseases. Here, 4-year follow-up studies demonstrated the long-term safety and clinical efficacy of SCE therapy for the treatment of T1D and T2D. Mechanistic studies found that the nature of platelets was modulated in diabetic subjects after receiving SCE therapy. Platelets and their released mitochondria display immune tolerance-associated markers that can modulate the proliferation and function of immune cells. Notably, platelets also expressed embryonic stem cell- and pancreatic islet β-cell-associated markers that are encoded by mitochondrial DNA. Using freshly-isolated human pancreatic islets, ex vivo studies established that platelet-releasing mitochondria can migrate to pancreatic islets and be taken up by islet β cells, leading to the proliferation and enhancement of islet β-cell functions. These findings reveal new mechanisms underlying SCE therapy and open up new avenues to improve the treatment of diabetes in clinics. Stem Cells Translational Medicine 2017;6:1684-1697.
Nuclear Translocation and Activation of the Transcription Factor NFAT Is Blocked by Herpes Simplex Virus Infection (2001)

Transcription factors of the NFAT (nuclear factor of activated T cells) family are expressed in most immune system cells and in a range of other cell types. Signaling through NFAT is implicated in the regulation of transcription for the immune response and other processes, including differentiation and apoptosis. NFAT normally resides in the cytoplasm, and a key aspect of the NFAT activation pathway is the regulation of its nuclear import by the Ca(2+)/calmodulin-dependent phosphatase calcineurin. In a cell line stably expressing green fluorescent protein (GFP)-NFAT, this import can be triggered by elevation of intracellular calcium and visualized in live cells. Here we show that the inducible nuclear import of GFP-NFAT is efficiently blocked at early stages of herpes simplex virus (HSV) infection. This is a specific effect, since we observed abundant nuclear accumulation of a test viral protein and no impediment to general nuclear localization signal-dependent nuclear import and retention in infected cells. We show that virus binding at the cell surface is not itself sufficient to inhibit the signaling that induces NFAT nuclear translocation. Since the block occurs following infection in the presence of phosphonoacetic acid but not cycloheximide, we infer that the entry of the virion and early gene transcription are required but the effect is independent of DNA replication or late virus gene expression. A consequence of the block to GFP-NFAT import is a reduction in NFAT-dependent transcriptional activation from the interleukin-2 promoter in infected cells. This HSV-mediated repression of the NFAT pathway may constitute an immune evasion strategy or subversion of other NFAT-dependent cellular processes to promote viral replication.