gebhart/patrepeval huggingface dataset:
Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo (2018)

Cancer cells and embryonic tissues share a number of cellular and molecular properties, suggesting that induced pluripotent stem cells (iPSCs) may be harnessed to elicit anti-tumor responses in cancer vaccines. RNA sequencing revealed that human and murine iPSCs express tumor-associated antigens, and we show here a proof of principle for using irradiated iPSCs in autologous anti-tumor vaccines. In a prophylactic setting, iPSC vaccines prevent tumor growth in syngeneic murine breast cancer, mesothelioma, and melanoma models. As an adjuvant, the iPSC vaccine inhibited melanoma recurrence at the resection site and reduced metastatic tumor load, which was associated with fewer Th17 cells and increased CD11b&#43;GR1hi myeloid cells. Adoptive transfer of T cells isolated from vaccine-treated tumor-bearing mice inhibited tumor growth in unvaccinated recipients, indicating that the iPSC vaccine promotes an antigen-specific anti-tumor T cell response. Our data suggest an easy, generalizable strategy for multiple types of cancer that could prove highly valuable in clinical immunotherapy.
Oncogenicity of the Developmental Transcription Factor Sox9 (2012)

SOX9 [sex-determining region Y (SRY)-box 9 protein], a high mobility group box transcription factor, plays critical roles during embryogenesis and its activity is required for development, differentiation, and lineage commitment in various tissues including the intestinal epithelium. Here, we present functional and clinical data of a broadly important role for SOX9 in tumorigenesis. SOX9 was overexpressed in a wide range of human cancers, where its expression correlated with malignant character and progression. Gain of SOX9 copy number is detected in some primary colorectal cancers. SOX9 exhibited several pro-oncogenic properties, including the ability to promote proliferation, inhibit senescence, and collaborate with other oncogenes in neoplastic transformation. In primary mouse embryo fibroblasts and colorectal cancer cells, SOX9 expression facilitated tumor growth and progression whereas its inactivation reduced tumorigenicity. Mechanistically, we have found that Sox9 directly binds and activates the promoter of the polycomb Bmi1, whose upregulation represses the tumor suppressor Ink4a/Arf locus. In agreement with this, human colorectal cancers showed a positive correlation between expression levels of SOX9 and BMI1 and a negative correlation between SOX9 and ARF in clinical samples. Taken together, our findings provide direct mechanistic evidence of the involvement of SOX9 in neoplastic pathobiology, particularly, in colorectal cancer.
THE EPIDEMIOLOGY AND GENETICS OF MULTIPLE SCLEROSIS (1999)

Epidemiological data suggest an aetiological role for both genetic and environmental factors in multiple sclerosis (MS), but these have yet to be fully characterized. It is probable that MS susceptibility is determined by polygenes but specific loci need to be identified.
Robust pose estimation through visual/GNSS mixing (2016)

The current paper is dealing with the use of a novel system that takes advantage of several sensors (Global Navigation Satellite System-GNSS, Inertial Measurement Units-IMU and Digital Imaging Sensors - DIS) that are integrated on a single platform to visualize and provide augmented pictures of underground infrastructure networks (electricity, water, district heating, irrigation, sewerage and communication networks) in a city to help their better management and maintenance. A special tablet equipped with GNSS and IMU sensors theoretically is able to give precise information of the position (X, Y, Z) and attitude (ω,φ,κ) of the camera embedded on the system. The offset (from the GNSS antenna) of the centre of the lens and the rotation of the camera coordinate system can be determined through a system calibration procedure therefore the pose estimation parameters of the image taken from the Digital Imaging Sensor can be determined. In the case where the 3D GIS is available, that is mapping except from the city's roads, pavements and buildings the underground facilities networks, the image obtained by the camera of the system integrating the GNSS and IMU sensors can provide an augmented image of the facilities networks. This image will then be used for better management and maintenance purposes of the underground features in the city. There is always the possibility that the GNSS system is not working properly due to satellite invisibility and multi path error which is common during its operation in the city centre. Additionally, the magnetometer embedded in IMU is prone to environmental noise and interference and produces erroneous results. In this case two alternative methods are used to determine the exact exterior orientation of the camera: conventional photogrammetric pose estimation ; vanishing lines pose estimation All the methods are available in the system and depending on the GNSS signal quality and the adequacy of the control conditions the user can switch from one method to the others.
Therapeutic angiogenesis for myocardial ischemia (2004)

Therapeutic angiogenesis offers promise as a novel treatment for ischemic heart disease, particularly for patients who are not candidates for current methods of revascularization. The goal of treatment is both relief of symptoms of coronary artery disease and improvement of cardiac function by increasing perfusion to the ischemic region. Protein-based therapy with cytokines including vascular endothelial growth factor and fibroblast growth factor demonstrated functionally significant angiogenesis in several animal models. However, clinical trials have yielded largely disappointing results. The attenuated angiogenic response seen in clinical trials of patients with coronary artery disease may be due to multiple factors including endothelial dysfunction, particularly in the context of advanced atherosclerotic disease and associated comorbid conditions, regimens of single agents, as well as inefficiencies of current delivery methods. Gene therapy has several advantages over protein therapy and recent advances in gene transfer techniques have improved the feasibility of this approach. The safety and tolerability of therapeutic angiogenesis by gene transfer has been demonstrated in phase I clinical trials. The utility of therapeutic angiogenesis by gene transfer as a treatment option for ischemic cardiovascular disease will be determined by adequately powered, randomized, placebo-controlled Phase II and III clinical trials. Cell-based therapies offer yet another approach to therapeutic angiogenesis. Although it is a promising therapeutic strategy, additional preclinical studies are warranted to determine the optimal cell type to be administered, as well as the optimal delivery method. It is likely the optimal treatment will involve multiple agents as angiogenesis is a complex process involving a large cascade of cytokines, as well as cells and extracellular matrix, and administration of a single factor may be insufficient. The promise of therapeutic angiogenesis as a novel treatment for no-option patients should be approached with cautious optimism as the field progresses.
A Principal Component Analysis for the Morphological Characters of Diploid and Triploid Populations of Lilium lancifolium in Korea (2006)

To clarify the morphological and geographical differentiation among the polyploid complexes of L. lancifolium collections in Korea, the mo게hological variation of 173 accessions were analyzed by ANOVA (one-way analysis of variance) and PCA (principal component analysis) on the basis of 38 morphological characters. 173m accessions were grouped into 78 diploids and 95 triploids by ploid levels and the triploids separated into 75 inland triploids (all around the Korea) and 20 island triploids (Backryung-do and Sochung-do, westemmost and northernmost islands of Korea) by geographic distribution and morphology. Island triploids showed significant morphological differences with inland triploids in ANOVA by many floral and leaf characters. In PCAs, diploids were separated from inland triploids by having longer plant height, smaller flower characters, higher pollen fertility and more stomata. The first four principal components accounted for 44.1% of the total variation. Plots of the island and inland groups for the first and second principal components separated each other with slight overlapping. Although the ploid forms are different between diploid and island triploid, island triploids were more closely overlapped with diploids by principal component 1 and 2 than inland triploids. This reflects that the whole external morphology of island triploids are similar to that of diploids. This, the phenotypic differentiation between inland and island triploids seems to be partly related to their geographical origins.
Kinetic and hydrodynamic effects in the activity testing of hydrodesulfurization catalysts in packed-bed reactors (1978)

In pilot-plant tests, an equation derived for the kinetic ''forcing function'' on the assumption of 1.65 order kinetics correlated well with the downflow hydrodesulfurization of a 529/sup 0/-1039/sup 0/F light Arabian vacuum gas oil containing 2.347Vertical Bar3< by wt of sulfur over 0.062 in. extrudate Exxon RT-2 catalyst in a 94.0 cm long fixed bed, over the same catalyst diluted with an equal amount of 46 mesh carborundum in a 146.0 cm long bed, or over 14-35 mesh crushed catalyst, again diluted, in a 166.0 cm long bed. Hydrodynamic effects had a significant effect on the results of the activity tests in downflow trickle beds. Catalyst wetting and/or liquid holdup effects influenced the apparent catalyst activity in all cases. Space velocity had the greatest influence on activity results, and particle size also had an effect; bed length was of lesser importance. However, three upflow tests over extrudate catalyst in beds 18.3-94.0 cm long indicated that consistent hydrodesulfurization activity measurements are obtained in long bed upflow reactors.
Arterial Stiffness as Underlying Mechanism of Disagreement Between an Oscillometric Blood Pressure Monitor and a Sphygmomanometer (2000)

Oscillometric blood pressure devices tend to overestimate systolic blood pressure and underestimate diastolic blood pressure compared with sphygmomanometers. Recent studies indicate that discrepancies in performance between these devices may differ between healthy and diabetic subjects. Arterial stiffness in diabetics could be the underlying factor explaining these differences. We studied differences between a Dinamap oscillometric blood pressure monitor and a random-zero sphygmomanometer in relation to arterial stiffness in 1808 healthy elderly subjects. The study was conducted within the Rotterdam Study, a population-based cohort study of subjects aged 55 years and older. Systolic and diastolic blood pressure differences between a Dinamap and a random-zero sphygmomanometer were related to arterial stiffness, as measured by carotid-femoral pulse wave velocity. Increased arterial stiffness was associated with higher systolic and diastolic blood pressure readings by the Dinamap compared with the random-zero sphygmomanometer, independent of age, gender, and average mean blood pressure level of both devices. The beta-coefficient (95% CI) was 0.25 (0.00 to 0.50) mm Hg/(m/s) for the systolic blood pressure difference and 0.35 (0.20 to 0.50) mm Hg/(m/s) for the diastolic blood pressure difference. The results indicate that a Dinamap oscillometric blood pressure device, in comparison to a random-zero sphygmomanometer, overestimates systolic and diastolic blood pressure readings in subjects with stiff arteries.
Use of methyl salicylate as a simulant to predict the percutaneous absorption of sulfur mustard (2001)

Exposure to chemical vesicants such as sulfur mustard (HD) continues to be a threat to military forces requiring protectant strategies to exposure to be evaluated. Methyl salicylate (MS) has historically been the simulant of choice to assess HD exposure. The purpose of this study was to compare the percutaneous absorption and skin deposition of MS to HD in the isolated perfused porcine skin flap (IPPSF). The HD data were obtained from a previously published study in this model wherein 400 microg cm(-2) of ](14)C[-MS or ](14)C[-HD in ethanol were topically applied to 16 IPPSFs and experiments were terminated at 2, 4 or 8 h. Perfusate was collected at increasing time intervals throughout perfusion. Radioactivity was determined in perfusate and skin samples. Perfusate flux profiles were fitted to a bi-exponential model Y(t) = A(e(-bt) - e(-dt)) and the area under the curve (AUC), peak flux and time to peak flux were determined. Sulfur mustard had more pronounced and rapid initial flux parameters (P < 0.05). The AUCs determined from observed and model-predicted parameters were not statistically different, although the mean HD AUC was 40--50% greater than MS. The HD skin and fat levels were up to twice those seen with MS, but had lower stratum corneum and residual skin surface concentrations (P < 0.05). Compared with other chemicals studied in this model, HD and MS cutaneous disposition were very similar, supporting the use of MS as a dermal simulant for HD exposure.
Multipath Ghosts in Radar Imaging: Physical Insight and Mitigation Strategies (2014)

Most algorithms commonly exploited for radar imaging are based on linear models that describe only direct scattering events from the targets in the investigated scene. This assumption is rarely verified in practical scenarios where the objects to be imaged interact with each other and with surrounding environment producing undesired multipath signals. These signals manifest in radar images as “ghosts" that usually impair the reliable identification of the targets. The recent literature in the field is attempting to provide suitable techniques for multipath suppression from one side and from the other side is focusing on the exploitation of the additional information conveyed by multipath to improve target detection and localization. This work addresses the first problem with a specific focus on multipath ghosts caused by target-to-target interactions. In particular, the study is performed with regard to metallic scatterers by means of the linearized inverse scattering approach based on the physical optics (PO) approximation. A simple model is proposed in the case of point-like targets to gain insight into the ghosts problem so as to devise possible measurement and processing strategies for their mitigation. Finally, the effectiveness of these methods is assessed by reconstruction results obtained from full-wave synthetic data.
Recent Patents on Electrospun Biomedical Nanostructures: An Overview (2008)

Nanostructures in the form of tubes, wires, crystals, rods, spheres, and fibers have been fabricated and assembled into various macrostructures for a variety of high technology applications. Nanofeatures impart several amazing properties to these macrostructures including high surface area, surface functionality, and superior mechanical, optical, electrical, and magnetic properties over the parent bulk material. Polymeric nanofibers in the form of nonwoven cloth, membrane, braids and tubes are extensively used for daily needs, and in addition used as filters, protective clothing, and for a variety of industrial and biomedical applications. Electrospinning or electrostatic spinning has emerged as a very popular technique to fabricate polymeric nanofiber matrices. More than 100 different polymers of natural, synthetic origin, their blends and composites have been electrospun into different three dimensional (3-D) macrostructures. Electrospinning provides opportunities to manipulate and control surface area, fiber diameter, porosity and pore size of nanofiber matrices. These nanofiber matrices closely mimic the structure of extracellular matrix (ECM) and influence cellular activities both in vitro and in vivo. Nanofiber macrostructures have been used as a vehicle to deliver therapeutic agents, as scaffolds for engineering various tissues and also serve as an integrated part of biomedical implants. Present review will cover some of the recent important patents that use electrospun nanofiber matrices for various biomedical applications.
Better alkylation control (1986)

The regulatory proteins coded by the human immunodeficiency virus, (HIV)-1 genome are expressed by the infected cells before the initiation of the synthesis of structural proteins and thus immune response directed against these proteins could destroy infected cells before the release of infectious virions. The evaluation of T-lymphocyte responses toward Tat, one of the main HIV-1 regulatory proteins, is therefore of interest. We selected a group of HIV-infected patients with retained response to the recall antigen purified protein derivative and tested their CD4+ helper T-cell response toward recombinant Tat and toward 12 soluble synthetic partially overlapping 15-16-mer Tat peptides in a proliferation assay. Three peptides (amino acids 17-32, 33-48, and 65-80) were significantly recognized by the helper T-cells from infected individuals but not by the nine HIV-1-negative control persons. Nine of the 14 patients (64%) responded to at least one of these Tat peptides. Of the identified immunodominant peptides containing T-cell epitopes, one (aa 65-80) was recognized in association with human leukocyte antigens DR-2 allele, while the others appeared to be promiscuous and were equally recognized in association with several DR molecules. The identified immunogenic peptides were analyzed for the predicted presence of T-cell antigenic sites by several algorithms and positive correlation was detected for each peptide. Our results thus indicate that Tat protein can induce a cell-mediated immune response and identify three peptides containing T-cell epitopes that may be of importance in vaccine development.
Degradation of Pentoses and Hexouronic Acids in Subcritical Water (2007)

Abstract The degradation of arabinose, xylose, ribose and lyxose in subcritical water was measured at 200, 220 and 240 °C. Ribose was the most rapidly degraded among the pentoses tested. The degradation of the glucuronic and galacturonic acids proceeded at lower temperatures than that of the pentoses, and was measured at 140, 150 and 160 °C. The degradation processes of the pentoses and hexouronic acids could be expressed by the Weibull model, and the kinetic parameters were then estimated. The activation energy and frequency factor for the degradation of each substrate were estimated from the temperature dependence of the rate constant. The enthalpy‐entropy compensation held for the degradation of the pentoses as well as the hexoses, which suggests that the degradation of the hexouronic acids proceeded through a mechanism different from that for the pentoses and hexoses. The molar yield of a pentose to furfural was ca. 0.3 at any temperature, irrespective of the pentose type. Acidic compounds were also formed from the pentoses in proportion to the amount of consumed substrates. The formation of acidic compounds resulted in a rapid decrease in pH.
Identification and characterization of a homologue of the pro‐inflammatory cytokine Macrophage Migration Inhibitory Factor in the tick, <i>Amblyomma americanum</i> (2001)

Studying tick feeding and digestion, we discovered in a cDNA library from partially fed Amblyomma americanum ticks the first known arthropod homologue of a human cytokine, the pro-inflammatory Macrophage Migration Inhibitory Factor (MIF). The tick origin of the MIF cDNA clone was confirmed by sequencing a genomic fragment that contained the full-length tick MIF gene with two introns. Antiserum to a tick MIF-specific peptide as well as antiserum to complete tick MIF revealed the expression of tick MIF in the salivary gland and midgut tissues of A. americanum ticks. In an in vitro functional assay, recombinant tick MIF inhibited the migration of human macrophages to the same extent that recombinant human MIF did.
Multi-protocol profiles to support user mobility across network technologies (2004)

Seamless roaming in heterogeneous circuit-switched and IP networks is key for successful migration towards all-IP. The Unified Mobility Manager (UMM) keeps track of users' locations whatever network they are roaming, and that routes calls from any originating to any terminating network. In a UMM enabled network, calls are addressed to users and dynamically routed to the active devices. This notion of user mobility breaks up the traditional coupling of addresses and devices, and replaces it with rules for the dynamic mapping from logical to device addresses. We present the UMM multi-protocol subscriber base that is built upon the concept of Incoming Call Handling (ICH) profiles that are the addressable entities in the UMM. An ICH profile contains all address level service data as well as the routing rules to the actual terminating devices. We also present our implementation of the UMM subscriber base in detail, and compare the UMM approach with two other approaches - a federated approach and an approach that combines ANSI-41 user mobility with GAIT interworking.
Use of the biased estimator in the interpretation of spectroscopic ellipsometry data (1991)

The use of the biased estimator in the fitting of spectroscopic ellipsometry data is examined and applied to data from two-channel polarization modulation ellipsometry experiments. It is pointed out that the use of the biased estimator, as opposed to the unbiased estimator that is usually found in the literature, allows the experimentalist to weight properly the more accurate parts of the spectrum, to switch among different representations of the data, and to calculate a goodness of fit. The fit to data taken on a 59-nm SiO(2) film on Si is examined with both the biased and the unbiased estimators.
Skin inflammation exacerbates food allergy symptoms in epicutaneously sensitized mice (2018)

Even after eliminating exposure to the antigen, inflammation from skin barrier disruption can exacerbate the severity of food allergy symptoms. Serum baseline mmcp1 levels might be an effective marker for predicting the severity of antigen-induced allergic symptoms.
Photocrosslinkable Hyaluronan-Gelatin Hydrogels for Two-Step Bioprinting (2010)

Bioprinting by the codeposition of cells and biomaterials is constrained by the availability of printable materials. Herein we describe a novel macromonomer, a new two-step photocrosslinking strategy, and the use of a simple rapid prototyping system to print a proof-of-concept tubular construct. First, we synthesized the methacrylated ethanolamide derivative of gelatin (GE-MA). Second, partial photochemical cocrosslinking of GE-MA with methacrylated hyaluronic acid (HA-MA) gave an extrudable gel-like fluid. Third, the new HA-MA:GE-MA hydrogels were biocompatible, supporting cell attachment and proliferation of HepG2 C3A, Int-407, and NIH 3T3 cells in vitro. Moreover, hydrogels injected subcutaneously in nude mice produced no inflammatory response. Fourth, using the Fab@Home printing system, we printed a tubular tissue construct. The partially crosslinked hydrogels were extruded from a syringe into a designed base layer, and irradiated again to create a firmer structure. The computer-driven protocol was iterated to complete a cellularized tubular construct with a cell-free core and a cell-free structural halo. Cells encapsulated within this printed construct were viable in culture, and gradually remodeled the synthetic extracellular matrix environment to a naturally secreted extracellular matrix. This two-step photocrosslinkable biomaterial addresses an unmet need for printable hydrogels useful in tissue engineering.