gebhart/patrepeval huggingface dataset:
Isolation and identification of muscle-derived stem cells in the rat penile corpus cavernosum (2012)

Objective To investigate the methods for isolation and identification of muscle-derived stem cells (MDSCs) in vitro.Methods The penile corpus cavemosums,from 2-month-old Sprague-Dawley rats,were digested and separated with0.5％ type Ⅰ collagenase and 0.1％ trypsin.Stem cells were isolated by the modified Preplate technique.The cells were cultured for 1 h,and the adherent cells were named PP1.The non-adherent cells were cultured in the other flask for 2 h,and adherent cells named PP2.Non-adherent cells were cultured again in the other flask for 18 h,and the adherent cells named PP3.Thereafter,non-adherent cells were cultured at intervals of 24 h in order to obtain the adherent cells PP4,PP5,PP6.The morphological changes of the cells were observed.The expression of stem cell marker (Sca-1) and desmin was detected by flow cytometry in PP3,PP6 cells; The positive expression of Sca-1 and desmin was detected by Western blotting in PP1-PP6 cells ; The expression of Sca-1,Desmin,Sca-1/ Desmin was detected by immunofluorescence cytochemistry in PP6 cells.Results The adherence of PP1- PP6 cells was decreased gradually.PP6 cells were smaller and round floating on the culture medium.Adherend cells could be seen and shaped round or spindly from day two to three.The positive rate of Sca-1 ( ± ) in PP3 cells was (0.3 ± 0.2) ％ ( P ＞ 0.05 ),and that of Desmin ( + ) was ( 15.3 ± 1.5 ) ％ ( P ＜0.05 ) in comparison to the controls.The positive rate of Sca-1 ( + ) in PP6 cells was (5.7 ±0.7)％,and that of Desmin ( + ) was (41.1 ± 1.6)％ (P＜0.05) in comparison to PP3 cells.Western blotting showed that there was no obvious expression of Sca-1 protein in PP1 -PP5 cells,but there was in pp6 cells.Desmin protein was detected in pp1-pp6 cells and increased gradially.Positive immunofluorescence outcomes were found with the stem cell markers and myoblast markers.The cells which expressed Sca-1 scattered in microscopic field of vision.Positive staining of Sca-1 mainly distributed in the cytoplasm.The cells expressing Desmin aggregated,and the positive staining of Desmin distributed in the cytoplasm.It was also found that very few cells were double-positive staining of Sca-1 and Desmin,which were stained in the cytoplasm simultaneously.Conclusion We separated the cells with phenotype of stem cells and myoblasts from rat penile corpus cavernosum.

Key words: 
Penile corpus cavernosum;  Muscle-derived stem cells;  Stem cell marker; Desmin
Complete experimental toolbox for alignment-free quantum communication (2012)

Quantum communication employs the counter-intuitive features of quantum physics for tasks that are impossible in the classical world. It is crucial for testing the foundations of quantum theory and promises to revolutionize information and communication technologies. However, to execute even the simplest quantum transmission, one must establish, and maintain, a shared reference frame. This introduces a considerable overhead in resources, particularly if the parties are in motion or rotating relative to each other. Here we experimentally show how to circumvent this problem with the transmission of quantum information encoded in rotationally invariant states of single photons. By developing a complete toolbox for the efficient encoding and decoding of quantum information in such photonic qubits, we demonstrate the feasibility of alignment-free quantum key-distribution, and perform proof-of-principle demonstrations of alignment-free entanglement distribution and Bell-inequality violation. The scheme should find applications in fundamental tests of quantum mechanics and satellite-based quantum communication.
The effects of pulsed introduction of buffer gas on ion storage and detection efficiencies in a quadrupole ion trap (1997)

The quadrupole ion trap is commonly operated with a constant background pressure of an inert, low molecular weight buffer gas. This inclusion of a buffer gas has been shown to increase the sensitivity and mass resolution of the instrument. Research to gain an understanding of these effects, both experimental and through simulations, has typically assumed that it is optimal to maintain a constant buffer gas pressure throughout the entire experiment This article describes the effects of the pulsed introduction of buffer gas at strategic points within the analytical scan and evaluates those events during which the presence of buffer gas is critical. By incorporating a pulsed valve within the ion trap manifold, both the presence and pressure of the buffer gas can be controlled and varied during the individual steps of the scan. The presence of helium buffer gas just before the ion ejection and detection event showed a greater increase in intensity of the ion signal than at any other time in the analytical scan. In addition, this increase in intensity upon pulsed introduction of buffer gas prior to detection is constant over a wide range of pulsed valve open times (i.e., pressures), whereas the signal enhancement upon pulsed introduction of the buffer gas before ionization is observed only over a narrow range of pulsed valve open times.
Development of a Need-Oriented Steam Turbine Cycle Simulation Toolbox (2005)

We call an electric generation mechanism, whose working fluid is water, as a steam turbine cycle or Rankine cycle. A steam turbine cycle has been adopted in fossil-fuel or nuclear power plants because it is suitable for large-scale and continuous operation. To check the performance level of a steam turbine cycle, performance tests are carried out according to the performance test codes (PTCs) provided by the authorized institute. However, authors found that it was too difficult to follow the PTCs in actual plants. The reason is signal reliability and the prerequisites of the PTCs. The effort to overcome these shortcomings already started and some commercial solutions were also developed. These solutions include a general-purpose simulation code with signal validation techniques. Despite these achievements, performance engineers are suffering from the inconsistency between their previous performance analysis work and new performance analysis tools. This study proposed the solution, a need-oriented turbine cycle simulation toolbox, which is another general-purpose simulation code with the signal validation method based on multivariate statistics. This toolbox removed the inconsistency by customizing the actual needs of performance engineers. The accuracy of the developed simulation code was validated with other commercial turbine cycle simulation codes.
Ultrasound Assessment of Residual Urine: A Quantitative Method (1985)

A method of measuring residual urine volume using ultrasound is described. The volume is computed from serial parallel sections of the bladder. This method is found to be significantly more accurate than previously reported techniques and is quick and easy to perform.
Intraoperative Functional MRI Using a Real-Time Neurosurgical Navigation System (1997)

A 42-year-old-man had focal left hand motor seizures. MR studies demonstrated a right posterior frontal brain tumor. Functional MRI was performed, localizing the motor cortex posterior to the lesion. The functional images were integrated with a neurosurgical navigation computer. A real-time intraoperative display of the anatomic and functional images was produced, registered to a neurosurgical probe. Excellent correlation was demonstrated between the functional maps and invasive electrophysiologic mapping performed at the time of craniotomy.
Concrete at the beginning of 21st century (2002)

The paper represents an overview of new trends in development and use of concrete with main characteristics being functionality and increased durability. This mainly refers to the high strength concrete. Such concrete is ensuring several times longer life of constructions than concrete used in the past. This is why theoretical and practical achievements of the so called Self-Compacting Concrete (SCC) worldwide should be analyzed. This above mentioned type shows better characteristics in comparison with the concrete used in the past when use of vibration was needed for its preparation and implementation. SCC eliminates the human factor while ensuring that concrete has designed characteristics. Self Compacting Concrete is a type to be used in the near future and should be introduced into civil engineering practice in the country. Furthermore this overview analyses world tendencies in utilization of the so called ecological (green) concrete, with reduced percentage of cement and therefore more environment friendly, while bonding characteristics of the pure cement are maintained by use of industrial waste, such as flying ash, slag, silica fume, etc.
Facilitated Catecholamine Transport through Bulk and Polymer-Supported Liquid Membranes (1996)

A series of crown boronic acids, 1−4, were synthesized and studied as carriers for catecholamine transport through bulk liquid membranes (BLMs) and supported liquid membranes (SLMs). Carrier 1 greatly facilitated the transport of primary catecholamines through BLMs; whereas, the more lipophilic analogues 3 and 4 were less effective. A combination of kinetic, mass spectral, and NMR evidence suggests that the transported species in BLMs is the cyclic, zwitterionic, 1:1 complex 7. The SLM transport studies used a liquid membrane of 2-nitrophenyl octyl ether supported by a thin, flat sheet of porous polypropylene. In the absence of carrier there was neglible dopamine transport (<5 × 10-9 mol/m2·s) at pH 7.2. When the membrane contained carrier 3 (33 mM or about 2% wt), facilitated catecholamine transport was observed in the order of dopamine (5 × 10-7 mol/m2·s) > epinephrine (1.5 × 10-7 mol/m2·s) > norepinephrine (0.8 × 10-7 mol/m2·s). SLMs containing carrier 3 were stable, implying that carrier 3 is a very good candidate for transport mechanism studies. Crown boronic acid 4 was an even better transport carrier of primary catecholamines with a transport order of norepinephrine (4.7 × 10-6 mol/m2·s) > dopamine (3.5 × 10-6 mol/m2·s) ≫ epinephrine (3 × 10-8 mol/m2·s). It is 10 times more effective than an equimolar mixture of boronic acid 5 and crown 6, which is one of best examples of ditopic cooperativity yet observed in SLM transport. SLMs containing 4, however, did not exibit long-term stability. Overall, it is possible that a device based on SLMs containing crown boronic acid carriers can be developed to selectively extract catecholamines from clinical samples.
Observation of an AB Phase in Bidisperse Nanocrystal Superlattices (2004)

Nanocrystal metamaterials: The self- assembly of ordered solids comprised of nanocrystals with different properties is of great interest. Ordered two- and three-dimensional nanocrystal superlattices with a rock salt structure (see graphic) were formed by drying a dispersion containing large magnetic (iron) and small noble metal (gold) nanocrystals with carefully controlled size ratios.
Force field for simulations of 1,2-dimethoxyethane and poly(oxyethylene) based upon ab initio electronic structure calculations on model molecules (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTForce field for simulations of 1,2-dimethoxyethane and poly(oxyethylene) based upon ab initio electronic structure calculations on model moleculesGrant D. Smith, Richard L. Jaffe, and Do Y. YoonCite this: J. Phys. Chem. 1993, 97, 49, 12752–12759Publication Date (Print):December 1, 1993Publication History Published online1 May 2002Published inissue 1 December 1993https://pubs.acs.org/doi/10.1021/j100151a021https://doi.org/10.1021/j100151a021research-articleACS PublicationsRequest reuse permissionsArticle Views866Altmetric-Citations140LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
AIT-082 as a potential neuroprotective and regenerative agent in stroke and central nervous system injury (1999)

The synthetic purine 4-[[3-(1,6 dihydro-6-oxo-9-purin-9-yl)-1-oxypropyl] amino] benzoic acid (AIT-082, Neotrofin, leteprinim potassium) possesses several biological properties of note: it stimulates outgrowth of neurites from PC12 cells and neurones, stimulates synthesis and/or release of neurotrophic factors from astrocytes, enhances nerve fibre regeneration in vivo and enhances of memory in animals and humans. AIT-082 also protects against glutamate neurotoxicity in vitro and in vivo, which has led to successful tests of AIT-082 in animal models of acute central nervous system injury. In such cases, AIT-082 probably functions by both acutely reducing glutamate excitotoxicity and, over a longer period, by enhancing neuronal sprouting and functional recovery.
An 80nm 4Gb/s/pin 32b 512Mb GDDR4 Graphics DRAM with Low-Power and Low-Noise Data-Bus Inversion (2007)

A 4Gb/s/pin 32b parallel 512Mb GDDR4 SDRAM is implemented in an 80nm DRAM process. It employs a data-bus inversion coding scheme with an analog majority voter insensitive to mismatch, which reduces peak-to-peak jitter by 21 ps and voltage fluctuation by 68mV. A dual duty-cycle corrector is proposed to average duty error, and tuning is added to the auto-calibration of driver and termination impedance.
Inhibition of cytochrome systems of heart muscle and certain bacteria by the antagonists of dihydrostreptomycin: 2-alkyl-4-hydroxyquinoline <i>N</i>-oxides (1956)

Antagonism of streptomycin activity by culture filtrates of Pseudononas aerugino8a was first reported by Sureau, Arquie, Boyer & Saviard (1948). The factor responsible for the antagonism was isolated The present paper describes an investigation of some of the biological properties of these compounds; part of this work has already been briefly reported (Lightbown & Jackson, 1954; Jackson & Light- bown, 1955).
Watermarks and Text Transformations in Visual Document Authentication (2007)

Integrity of digital documents is a very important issue if they are to be legally binding. Common solutions, like digital signatures or message authentication codes, are based on cryptography and require computers or similar hardware to be produced. They can be trusted only as long as the employed hardware can be trusted. We present a complementary scheme for document authentication in untrusted environment. The scheme combines visual authentication, human- recognizable watermarks, and image transformations, and is suitable also for larger text document. Because it relies on visual cryptography, it requires practically no computational power on the receiver side. To prevent potential attackers from obtaining signatures without author’s knowledge, we propose using a simple challenge-response protocol.
Methods of producing plasma enhanced chemical vapor deposition silicon nitride thin films with high compressive and tensile stress (2008)

Various methods of generating high stress in thin plasma enhanced chemical vapor deposition (PECVD) silicon nitride (SiN) films are reported. Besides the mainstream variation of plasma power and other process parameters, novel techniques such as creation of high density layers in multilayer PECVD structures or exposure of SiN films to ultraviolet radiation are shown to increase intrinsic film stress. Thin PECVD SiN films have been analyzed by a variety of analytical techniques including Fourier transform infrared spectroscopy, x-ray reflectivity (XRR), time of flight secondary ion mass spectrometry, and transmission electron microscopy to collect data on bonding, density, chemical composition, and film thickness. The level of bonded hydrogen as well as film density has been found to correlate with film stress. Creation of multilayer structures and high density layers help to build up more stress compared to a standard single layer film deposition. Both the density and number of layers in a film, characterized by XRR, affect the stress. Higher density layers affect diffusion profiles and show impurity oscillations corresponding to a multilayer film structure. Ultraviolet cure allows the film to achieve higher level of tensile stress at relatively low temperatures (400–500°C), comparable to the result of film high temperature annealing.
[Use of 2-mercaptoethanol in cell culture]. (1992)

Survival and growth in in vitro cultivation of lymphocytes, lymphoma cells and some other cells including human carcinomas are profoundly improved by 2-mercaptoethanol. These cells hardly take up cystine, an essential nutrient in the culture medium, but in the presence of 2-mercaptoethanol they can utilize cystine. Recently it has been found that 2-mercaptoethanol is effective in the in vitro cultivation of pathogenic trypanosomes and in the in vitro development of bovine embryos. The mechanisms by which 2-mercaptoethanol improves the survival and growth of these cells are described.
Classification of seismic waveforms by integrating ensembles of neural networks (2002)

The problem considered is the discrimination between natural and artificial seismic events, based on their waveform recording. We build a classification environment consists of several ensembles of neural networks trained on boot-strap sample sets, using various data representations and architectures. The integration of the different ensembles is made in a nonconstant signal adaptive manner, using a posterior confidence measure based on the agreement (variance) within the ensembles. The proposed integrated classification machine achieved 92.1% correct classification on the seismic test data. Cross validation tests and comparisons indicate that such integration of a collection of ANN's ensembles is a robust way for handling high dimensional problems with a complex nonstationary signal space as in the current seismic classification problem.
Some optical properties of KTP, LiIO/sub 3/, and LiNbO/sub 3/ (1988)

Measurements of the absorption coefficient for KTP, LiIO/sub 3/, and LiNbO/sub 3/ are discussed. The variation of the refractive index with temperature has been measured for KTP and LiIO/sub 3/. It is necessary to know both the absorption coefficient beta and the variation in the indexes of refraction with temperature change dn/dT to determine the average power limit of a nonlinear interaction. With the dn/dT information, it is also possible to estimate the temperature half width of any nonlinear interaction by calculating the variation of the phase-matching condition with temperature.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A North American Survey of Intravenous Regional Anesthesia (1997)

One thousand questionnaires concerning the techniques and complications of intravenous regional anesthesia (IVRA) were sent to 900 American and 100 Canadian anesthesiologists.Of the 321 respondents, 86% perform IVRA regularly. A wide variation in devicerelated and clinical aspects was found, ranging from acceptable to falling outside published guidelines. Anesthesiologists perform a median of four upper-limb IVRA procedures per month, most often using 50 mL of lidocaine 0.5% at tourniquet pressures of 250 mm Hg or 100 mm Hg greater than the systolic blood pressure. Forearm, thigh, and calf IVRA are occasionally used. Complications, reported infrequently in the literature, were reported by respondents, including mistaken deflation of the cuff; dysphoria, dizziness, or facial tingling; seizures; cardiac arrests; and deaths. Although there was no correlation between complications and deviation from traditional practice, we recommend that IVRA be performed following recognized protocols by anesthesiologists who are familiar with the technique and trained to treat its potential complications. We recommend a protocol for IVRA. Implications: Intravenous regional anesthesia is a widely used anesthetic technique. A survey of 321 American and Canadian anesthesiologists indicates a wide variation in technique. Despite no correlation between complications and technique, the authors recommend that recognized protocols be used for this technique. (Anesth Analg 1997;85:858-63)
Facial landmark localization based on hierarchical pose regression with cascaded random ferns (2013)

The main challenge of facial landmark localization in real-world application is that the large changes of head pose and facial expressions cause substantial image appearance variations. To avoid high dimensional regression in the 3D and 2D facial pose spaces simultaneously, we propose a hierarchical pose regression approach, estimating the head rotation, facial components and landmarks hierarchically. The regression process works in a unified cascaded fern framework. We present generalized gradient boosted ferns (GBFs) for the regression framework, which give better performance than traditional ferns. The framework also achieves real time performance. We verify our method on the latest benchmark datasets. The results show that it outperforms state-of-the-art methods in both accuracy and speed.
Physical mapping of human insulin-like growth factor-I using specific monoclonal antibodies (1997)

The primary structure of recombinant human (h) insulin-like growth factor-I (IGF-I) epitopes recognized by a panel of 28 monoclonal antibodies (mAbs) is characterized. Pairwise mAb epitope mapping defines eight 'epitopic clusters' (I-VIII) which cover nearly the entire solvent-exposed IGF-I surface. Monoclonal antibody reactivity with 32 overlapping synthetic peptides and with IGF-I mutants is used to associate these epitopic clusters with the probable primary IGF-I sequences recognized. Epitopic cluster I involves residues in the C-domain and the first alpha-helix of the A-domain; clusters II, V and VII involve principally the B-domain; clusters III and IV map to amino acid sequences (55-70) and (1-13) respectively; cluster VI includes the A- and B-domains; and cluster VIII involves mainly the C-terminal part of the B-domain. Data indicate that this mAb panel defines 14 distinct IGF-I epitopes. The specific inhibition of HEL 92.1.7 IGF-I-promoted proliferation by these mAbs was explored. Direct correlation between mAb affinity and inhibitory activity was observed except in the case of clusters III- and VIII-specific mAbs. Finally, the combination of epitopic cluster I and II mAbs detect 0.5-10 ng/ml hIGF-I in a sandwich immunoassay, with no IGF-II crossreactivity. These anti-IGF-I mAbs are, therefore, useful for both the inhibition of IGF-I mitogenic activity and for the quantification of this growth factor. The potential use of this mAb panel in tumor cell growth control is discussed.