gebhart/patrepeval huggingface dataset:
Functional Studies on the C-Type Lectin Receptor CD302 Present on Dendritic Cells and Macrophages (2015)

Abstract Introduction: C-type lectin receptors (CLR) play an important role in the immune system by recognising molecular patterns expressed by exogenous and endogenous threats. They have been shown to capture and internalise antigens and to mediate other important immune cell functions. DEC205 and CLEC9A are being actively investigated as targets for clinical therapeutic cancer vaccines. We discovered CD302 as a new CLR expressed on human dendritic cells (DC), monocytes and macrophages (J Immunol 2007;179:6052). Our initial studies suggested the molecule could play a role in cell adhesion or migration due to its co-localisation with migratory structures on macrophages. Our study set out to investigate the potential immunological function of CD302 using mouse models and to define its wider tissue expression in man. Methods: We generated CD302 knockout (KO) mice lacking exon 1 of its gene, abrogating transcription, for functional studies. We characterised the transcriptional expression of CD302 in mouse immune cells using real-time PCR. We developed monoclonal mAb to mCD302. Human studies utilized the anti-CD302 mAbs, MMRI-20 & 21 in flow cytometry and confocal microscopy studies of human immune cell populations. Results: CD302 was primarily expressed in mouse liver, lungs, lymph nodes (LN) and spleen. In spleen, macrophages, granulocytes and dendritic cells (DC) expressed CD302. Analysis of LN DC subsets revealed 2.5-fold higher CD302 mRNA expression in migratory compared to resident DC populations. Enumeration of various immune populations in lymphoid organs by flow cytometry uncovered a modest deficiency in migratory DC number and proportion within LN of CD302 KO mice compared to wild-type (WT) mice. In vitro studies showed CD302 KO and WT DC had an equivalent capacity to be activated by various stimuli, prime T cells and migrate towards the lymphoid-homing chemokines CCL19/CCL21. CD302 KO migratory DC exhibited a reduced in vivo migratory capacity to LN after FITC skin-painting. However, CD302 KO macrophages migrated similarly to WT macrophages in vivo in response to thioglycollate. In man, CD302 was present in high density in liver and peripheral blood monocytes and myeloid but not plasmacytoid DC. Current studies are aimed at clarifying its distribution on tissue DC and macrophage subsets. Anti-CD302 coated microbeads were taken up by human monocyte derived macrophages and anti-CD302 mAb was also internalized by DC. Confocal studies showed that CD302 co-localized with F-actin structures at the near basal surface such as filopodia and lamellipodia and podosomes of human macrophages and EGFP tagged CD302 expressed in COS-1 cells associated with F-actin. Conclusion: Our data suggests that CD302 may play a specialist role in DC and macrophage membrane functions. This appears to relate to its ability to associate with F-actin and may contribute to the membrane interactions required for DC to migrate towards the draining LN. Disclosures Hart: DendroCyte BioTech Pty Ltd: Equity Ownership. Clark:DendroCyte BioTech Pty Ltd: Equity Ownership.
Optical approach to resin formulation for 3D printed microfluidics (2015)

Microfluidics imposes different requirements on 3D printing compared to many applications because the critical features for microfluidics consist of internal microvoids. Resins for general 3D printing applications, however, are not necessarily formulated to meet the requirements of microfluidics and minimize the size of fabricated voids. In this paper we use an optical approach to guide custom formulation of resins to minimize the cross sectional size of fabricated flow channels as exemplars of such voids. We focus on stereolithgraphy (SL) 3D printing with Digital Light Processing (DLP) based on a micromirror array and use a commercially available 3D printer. We develop a mathematical model for the optical dose delivered through the thickness of a 3D printed part, including the effect of voids. We find that there is a fundamental trade-off between the homogeneity of the optical dose within individual layers and how far the critical dose penetrates into a flow channel during fabrication. We also experimentally investigate the practical limits of flow channel miniaturization given the optical properties of a resin and find that the minimum flow channel height is ~3.5-5.5<i>h<sub>a</sub></i> where <i>h<sub>a</sub></i> is the optical penetration depth of the resin, and that the minimum width is 4 pixels in the build plane. We also show that the ratio of the build layer thickness to <i>h<sub>a</sub></i> should be in the range 0.3-1.0 to obtain the minimum flow channel height for a given resin. The minimum flow channel size that we demonstrate for a custom resin is 60 <i>μ</i>m × 108 <i>μ</i>m for a 10 <i>μ</i>m build layer thickness. This work lays the foundation for 3D printing of <100 <i>μ</i>m microfluidic features.
An approach to environmental psychology (1974)

Environmental psychology, though a fast-growing field, is one of the most difficult to fit into the confines of scientific inquiry. Measuring such subjective data as reactions to color, heat, light, and sound would seem to be an almost impossible task; indeed, until now there has been no theory around which the research in this field could be organized. This volume represents a preliminary effort to identify the relevant variables involved and fit them into a systematic framework. Furthermore, it presents extensive sets of measures for investigating the theory and implementing it in a variety of everyday environments.Basically, the framework outlined here proposes that environmental stimuli are linked to behavioral responses by the primary emotional responses of arousal, pleasure, and dominance. By considering the impact of the environment on these basic emotional responses, the effects of diverse stimulus components within or across sense modalities can be readily compared. An additional concept, information rate, is used to compare the effects of different environments, each with stimulation in many sense modalities. In the final chapters the authors present a series of hypotheses which relate the emotional response variables to a diversity of behaviors such as physical approach, performance, affiliation, and verbally or nonverbally expressed preference.
Use of Amine Additives to Prevent Drying Loss on Aging (1950)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of Amine Additives to Prevent Drying Loss on AgingA. C. Zettlemoyer and Donald M. NaceCite this: Ind. Eng. Chem. 1950, 42, 3, 491–495Publication Date (Print):March 1, 1950Publication History Published online1 May 2002Published inissue 1 March 1950https://pubs.acs.org/doi/10.1021/ie50483a027https://doi.org/10.1021/ie50483a027research-articleACS PublicationsRequest reuse permissionsArticle Views57Altmetric-Citations8LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
The Application of High-Modulus Fibers to Ballistic Protection (1973)

Abstract A new family of experimental organic fibers (X-500) produced by the Monsanto Research Corporation has been evaluated ballistically. The development of this family of fibers made available for the first time a series of organic fibers with moduli and heac resistance more nearly comparable to inorganic fibers such as glass. Tensile analysis of three material types tested in yarn form at conventional loading rates showed physical properties ranging from typically brittle (10.1 g, den tenacity, 2.4% extension, and 550 g, den modulus) to moderately ductile (5.4 g, den tenacity, 15.4% extension, and 135 g/den modulus). The three samples were evaluated ballistically in fabric, felt, and laminate form. The most ductile sample (Type III) showed considerable promise with a ballistic resistance significantly greater than normal for a material of such modest tensile strength. Tensile recovery tests on this Type-III material showed a large amount of permanent set compared to most commercially available yarns. It appears that this capacity for plastic flow, combined with the inherent high modulus, act in combination to provide good energy absorption. Scanning electron micrographs of ballistic missile impact areas showed for the more brittle yarns, longitudinal splitting with little evidence of plastic deformation, or fusion of fibers around the hole produced by the missile. The more ductile yarns exhibited a combination of some longitudinal splitting with plastic deformation and fusion around the hole. This latter combination of energy absorbing mechanisms proved to more effective.
The Effects of Allopurinol and Superoxide Dismutase in a Rat Model of Skin Flap Necrosis (1989)

Oxygen-free radicals have been implicated as mediators of ischemic damage in a number of tissues, including heart, kidney, small intestine, and skin. Superoxide dismutase, a free radical scavenger, and allopurinol, an inhibitor of xanthine oxidase (a catalyst in the formation of superoxide) have been shown separately to decrease ischemic damage to tissue. Sixty-two male Sprague-Dawley rats (220 to 280 g) were divided into five groups: control, superoxide dismutase only, allopurinol only, high-dose combined allopurinol and superoxide dismutase, and low-dose combined allopurinol and superoxide dismutase. An 18-cm2 ventral island skin flap, based on a single inferior epigastric vessel, was raised and replaced. Blood flow was assessed with a perfusion fluorometer immediately after the flap was replaced, and on postoperative days 1 and 3. Gross necrosis was assessed on postoperative day 7. Gross necrosis was reliably reduced in all groups as compared with the control; however, necrosis in any one experimental group was not significantly different from any other experimental group. Necrosis (expressed as a percentage of the area of the random [distal] half of the flap) was as follows for each group: control, 74%; superoxide dismutase, 43%; allopurinol, 43%; high-dose combined, 48%; low-dose combined, 33%. Blood flow, as represented by the dermofluorescence index, was not changed by any of the treatments. Blood flow was also related to the eventual survival or necrosis for any one portion of tissue. All experimental groups survived with significantly less blood flow than the control.(ABSTRACT TRUNCATED AT 250 WORDS)
Absorption and scattering in ground‐penetrating radar: Analysis of the Bishop Tuff (2006)

Ground‐penetrating radar (GPR) signals are attenuated by both absorption and scattering. We performed low‐frequency (<100 MHz) GPR surveys at the Volcanic Tableland of the Bishop (California) Tuff to evaluate the factors that control GPR depth of investigation and to develop insight into the capabilities of such radars for Mars. The subsurface reflection character was very different for two different commercial systems used; together, they revealed both internal welding contacts in the tuff and an abundance of discrete scatterers. Attenuation coefficients were computed from profiles that showed distributed scattering: the semilogarithmic signal decay is directly analogous to seismic coda. The absorption (intrinsic loss) was determined to be ∼1 dB/m from low‐frequency vertical‐electric soundings. The residual attenuation (that is, the attenuation in the absence of absorption) is attributed to scattering. Scattering attenuation of ∼1 dB/m at 25–50 MHz corresponds to mean‐free paths as short as 4 m, a fraction of the two‐way propagation distances of 20–40 m. Therefore the Bishop Tuff is formally a strong scatterer to GPR. The mean‐free path is also comparable to the subsurface radar wavelength in this case, maximizing scattering loss. The scatterers themselves likely originate as welding heterogeneities; contrasts in dielectric constant due to density differences may be supplemented by moisture variations. On Mars, scattering is likely to contribute significant losses to GPR signals in all but the most uniform materials, and unfrozen thin films of water in the lower cryosphere could influence both absorption and scattering.
Tempo-Spatial Dynamics of Adult Plum Curculio (Coleoptera: Curculionidae) Based on Semiochemical-Baited Trap Captures in Blueberries (2017)

Plum curculio, Conotrachelus nenuphar (Herbst), has become an important pest of highbush blueberries in the northeastern United States. Here, we conducted experiments in 2010-2013 to compare the efficacy of semiochemical-baited traps for C. nenuphar versus conventional (beating cloth) sampling methods in blueberries, and to understand the seasonal abundance and distribution of C. nenuphar adults within and among blueberry fields using these traps. Black pyramid traps baited with the C. nenuphar aggregation pheromone grandisoic acid and the fruit volatile benzaldehyde caught three to four times more adults than unbaited traps without causing an increase in injury to berries in neighboring bushes. Numbers of adult weevils caught in traps correlated with those on bushes (beating cloth samples), indicating that trap counts can predict C. nenuphar abundance in the field. Early in the season, traps placed 20 m from field edges near a forest caught higher C. nenuphar numbers than traps placed at farther distances, suggesting movement of overwintered weevils from outside fields. Using a trapping network across multiple fields in an organic farm, we found evidence of C. nenuphar aggregation in "hotspots"; early in the season, C. nenuphar numbers in traps were higher in the middle of fields, and there was a correlation between these numbers and distance from the forest in 2013 but not in 2012. These results show that semiochemical-baited traps are effective in capturing C. nenuphar adults in blueberries, and that these traps should be placed in the interior of fields preferably, but not exclusively, near wooded habitats to maximize their efficacy.
Influence of Microbubbles on Free Radical Generation by Ultrasound in Aqueous Solution: Dependence of Ultrasound Frequency (2015)

The influence of microbubbles on sonochemical efficiencies has been investigated under 28, 45, and 100 kHz ultrasound irradiation. For the 28 and 100 kHz ultrasound frequencies, microbubbles suppressed the I3(-) formation from KI solution as well as the 7-hydroxycoumarin formation from coumarin solution caused by the ultrasonic irradiation. On the other hand, for the 45 kHz ultrasound frequency, microbubbles enhanced the I3(-) formation from KI solution as well as 7-hydroxycoumarin formation from coumarin solution caused by the ultrasonic irradiation. Detection of H2O2 after the irradiation of ultrasound in the presence or absence of microbubbles was also performed, and it was found that H2O2 formation was enhanced only when microbubbles were introduced under the 45 kHz ultrasonic irradiation, which was in good agreement with the results of KI oxidation dosimetry measurements and of coumarin fluorescent probe measurements. Based on these present results, plausible mechanisms that explain the dependence of the ultrasound frequency on the enhancement and suppression of free radical formation in the presence of MBs were proposed.
Industrial potential of lipoxygenases (2015)

Lipoxygenases (LOXs) are iron- or manganese-containing oxidative enzymes found in plants, animals, bacteria and fungi. LOXs catalyze the oxidation of polyunsaturated fatty acids to the corresponding highly reactive hydroperoxides. Production of hydroperoxides by LOX can be exploited in different applications such as in bleaching of colored components, modification of lipids originating from different raw materials, production of lipid derived chemicals and production of aroma compounds. Most application research has been carried out using soybean LOX, but currently the use of microbial LOXs has also been reported. Development of LOX composition with high activity by heterologous expression in suitable production hosts would enable full exploitation of the potential of LOX derived reactions in different applications. Here, we review the biological role of LOXs, their heterologous production, as well as potential use in different applications. LOXs may fulfill an important role in the design of processes that are far more environmental friendly than currently used chemical reactions. Difficulties in screening for the optimal enzymes and producing LOX enzymes in sufficient amounts prevent large-scale application so far. With this review, we summarize current knowledge of LOX enzymes and the way in which they can be produced and applied.
15N and 18O kinetic isotope effects in the thermal decomposition of N2O catalyzed by bromine (1993)

15N and 18O kinetic isotope effects (KIEs) in the thermal decomposition of N2O catalyzed by bromine were experimentally determined in the temperature range 773–873 K, resulting in KIE (15N)=−2.07+4020/T and KIE (18O)=−0.41+3290/T. For theoretical interpretation, based on the Bigeleisen formalism, the following planar transition states were taken into account: trans (N–N–O–Br)‡, trans (Br–N–N–O)‡, and branched (N–N&lt;OBr)‡. In addition to KIE, activation energy according to the Sanderson bond-energy–bond-order relationship and pre-exponential factor were calculated as subsidiary parameters in selecting an appropriate transition state among all probable configurations. The result reveals that it is meaningless to speculate whether the Br atom approaches the central or the terminal N atom of the N2O molecule, since both transition states could generate acceptable values of the kinetic isotope effects, activation energy, and pre-exponential factor.
<title>Synthetic vision as an integrated element of an enhanced vision system</title> (2002)

Enhanced Vision Systems (EVS) and Synthetic Vision Systems (SVS) have the potential to allow vehicle operators to benefit from the best that various image sources have to offer. The ability to see in all directions, even in reduced visibility conditions, offers considerable benefits for operational effectiveness and safety. Nav3D and The Boeing Company are conducting development work on an Enhanced Vision System with an integrated Synthetic Vision System. The EVS consists of several imaging sensors that are digitally fused together to give a pilot a better view of the outside world even in challenging visual conditions. The EVS is limited however to provide imagery within the viewing frustum of the imaging sensors. The SVS can provide a rendered image of an a priori database in any direction that the pilot chooses to look and thus can provide information of terrain and flight path that are outside the purview of the EVS. Design concepts of the system will be discussed. In addition the ground and flight testing of the system will be described.
Inhibition studies of some serine and thiol proteinases by new leupeptin analogs (1993)

Fifteen tripeptide analogues of leupeptin containing either a C-terminal argininal or lysinal were synthesized. The synthetic analogues were tested, using spectrophotometric assay techniques, as inhibitors of trypsin, kallikrein, thrombin, plasmin, and cathepsin B. The lysinal analogues were fairly selective as inhibitors of cathepsin B activity. Acetyl-L-leucyl-L-valyl-L-lysinal (21) showed a stronger inhibition of cathepsin B (IC50 = 4 nanomolar) than leupeptin. Acetyl-L-phenylalanyl-L-valyl-L-argininal (2i) was found to be a good inhibitor of cathepsin B (IC50 = 0.039 microM), thrombin (IC50 = 1.8 microM), and plasmin (IC50 = 2.2 microM).
Real-Time Temperature Sensor Based on In-Fiber Fabry–Perot Interferometer Embedded in a Resin (2018)

A simple to fabricate, cost-effective, robust, and highly accurate fiber optic temperature sensor is proposed and experimentally demonstrated. The sensor head consists of an in-fiber Fabry-Perot interferometer (FFPI) embedded in a mold made of a polyester resin. FFPI, which in general present good strain sensitivity and low-temperature sensitivity, was assembled by splicing a microsegment of capillary fiber of 14.66 μm between two single-mode fibers. The reflected spectrum of the FFPI exhibited a single interference dip within a span of 80 nm. The dimensions of the resin mold can be modified by changing the ambient temperature, which in turn changes the length of the FFPI air-cavity. The dip of the reflected spectrum is shifted toward shorter (larger) wavelengths when external temperature is decreased (increased). The large free spectral range (FSR) allowed to monitor the temperature changes by either a wavelength or an intensity interrogation scheme. Using a laser and an optical power meter it was possible to demonstrate a real-time temperature sensor with a sensitivity of -60.79 nW/°C and a resolution of 0.5 °C, in the range of 10-30 °C. We believe that the practicality of this real-time sensor can be very attractive for environmental sensing application.
BRAF V600E mutation in early-stage multiple myeloma: good response to broad acting drugs and no relation to prognosis (2015)

In this study, we analyzed the prevalence and clone size of BRAF V600E mutation in 209 patients with multiple myeloma and related the results to clinical phenotype, response and survival. Biopsies were screened for BRAF V600E by allele-specific real-time PCR (AS-PCR). Positive results were confirmed by immunohistochemistry, Sanger sequencing and, in three patients from whom we had stored purified myeloma cells, whole-exome sequencing. Eleven patients (5.3%) were BRAF V600E mutation positive by AS-PCR and at least one other method. The fraction of mutated cells varied from 4 to 100%. BRAF V600E-positive patients had no characteristic clinical phenotype except for significantly higher levels of serum creatinine (125 versus 86 μmol/l) Seven of eleven patients responded with at least very good partial response to alkylators, immunomodulatory agents or proteasome inhibitors. Progression-free and overall survival were similar in patients with and without the mutation. By this integrated approach, we found that patients with BRAF V600E mutation responded very well to broad acting drugs and there was no relation to prognosis in early-stage myeloma. In particular, a large mutated cell fraction did not correlate with aggressive disease.
CN&gt;inf&lt;0&gt;/inf&lt;Estimation and Near-Far Mitigation for GNSS Indoor Receivers (2005)

This paper covers two key points in indoor and dense-urban positioning using Global Navigation Satellite Systems: CN/sub 0/ estimation, and near-far estimation problem. For the former, a novel CN/sub 0/ estimator adequate to deal with the low signal levels encountered in indoor and dense-urban environments is described. For the latter, the results of a novel near-far problem mitigation technique are presented. Both simulations and live data have been used to illustrate the advantages of the proposed algorithms.
Laser Stimulation of Nerve Cells in <i>Aplysia</i> (1971)

Laser radiation at 488 nanometers selectively stimulates neurons in the abdominal ganglion of the marine mollusk Aplysia californica. The laser radiation can be scanned over the surface of the ganglion and can be effectively utilized in mapping cellular interconnections. The laser appears to cause these changes through some mechanism other than damage.