gebhart/patrepeval huggingface dataset:
The Genes Coding for Enterocin EJ97 Production by <i>Enterococcus faecalis</i> EJ97 Are Located on a Conjugative Plasmid (2003)

Enterococcus faecalis EJ97 produces a cationic bacteriocin (enterocin EJ97) of low molecular mass (5,327.7 Da). The complete amino acid sequence of enterocin EJ97 was elucidated after automated microsequencing of oligopeptides generated by endoproteinase GluC digestion and cyanogen bromide treatment. Transfer of the 60-kb conjugative plasmid pEJ97 from the bacteriocinogenic strain E. faecalis EJ97 to E. faecalis OG1X conferred bacteriocin production and resistance on the recipient. The genetic determinants of enterocin EJ97 were located in an 11.3-kb EcoRI-BglII DNA fragment of pEJ97. This region was cloned and sequenced. It contains the ej97A structural gene plus three open reading frames (ORFs) (ej97B, ej97C, and ej97D) and three putative ORFs transcribed in the opposite direction (orfA, orfB, and orfC). The gene ej97A translated as a 44-amino-acid residue mature protein lacking a leader peptide with no homology to other bacteriocins described so far. The product of ej97B (Ej97B) shows strong homology in its C-terminal domain to the superfamily of bacterial ATP-binding cassette transporters. The products of ej97C (Ej97C) and ej97D (Ej97D) could be proteins with 71 and 64 residues, respectively, of unknown functions and with no significant similarity to known proteins. There are two additional ORFs (ORF1 and ORF6) flanking the ej97 module, which have been identified as a transposon-like structure (tnp). ORF1 shows similarities to transposase of the Lactococcus lactis element ISS1 and is up to 50% identical to IS1216. This is flanked by two 18-bp inverted repeats (IRs) that are almost identical to those of ISS1 and IS1216. ORF6 (resEJ97) shows strong homology to the resolvase of plasmid pAM373 and up to 40 to 50% homology with the recombinase of several multiresistant plasmids and transposons from Staphylococcus aureus and E. faecalis. These data suggest that EJ97 could represent a new class of bacteriocins with a novel secretion mechanism and that the whole structure could be a composite transposon. Furthermore, two additional gene clusters were found: one cluster is probably related to the region responsible for the replication of plasmid pEJ97, and the second cluster is related to the sex pheromone response. These regions showed a high homology to the corresponding regions of the conjugative plasmids pAM373, pPD1, and pAD1 of E. faecalis, suggesting that they have a common origin.
Phosphate Is Essential for Inhibition of the Mitochondrial Permeability Transition Pore by Cyclosporin A and by Cyclophilin D Ablation (2008)

Energized mouse liver mitochondria displayed the same calcium retention capacity (a sensitive measure of the propensity of the permeability transition pore (PTP) to open) irrespective of whether phosphate, arsenate, or vanadate was the permeating anion. Unexpectedly, however, phosphate was specifically required for PTP desensitization by cyclosporin A (CsA) or by genetic inactivation of cyclophilin D (CyP-D). Indeed, when phosphate was replaced by arsenate, vanadate, or bicarbonate, the inhibitory effects of CsA and of CyP-D ablation on the PTP disappeared. After loading with the same amount of Ca(2+) in the presence of arsenate or vanadate but in the absence of phosphate, the sensitivity of the PTP to a variety of inducers was identical in mitochondria from wild-type mice, CyP-D-null mice, and wild-type mice treated with CsA. These findings call for a reassessment of conclusions on the role of the PTP in cell death that are based on the effects of CsA or of CyP-D ablation.
One-Health: a Safe, Efficient, Dual-Use Vaccine for Humans and Animals against Middle East Respiratory Syndrome Coronavirus and Rabies Virus (2016)

Rabies virus-based vectors have been proven to be efficient dual vaccines against rabies and emergent infectious diseases such as Ebola virus. Here we show that inactivated rabies virus particles containing the MERS-CoV S1 protein induce potent immune responses against MERS-CoV and RABV. This novel vaccine is easy to produce and may be useful to protect target animals, such as camels, as well as humans from deadly MERS-CoV and RABV infections. Our results indicate that this vaccine approach can prevent disease, and the RABV-based vaccine platform may be a valuable tool for timely vaccine development against emerging infectious diseases.
A New Prototype Modulator for the European XFEL Project in Pulse Step Modulator Technology (2010)

The European XFEL project at DESY in Germany requires 27 RF stations capable of 10 MW RF power each. Each RF station needs one high voltage modulator that generates pulses up to 12 kV and 2 kA with a duration of 1.7 ms and a nominal repetition rate of 10 Hz. DESY decided to investigate new modulator prototypes and Thomson has been awarded to design and build one of these prototype modulators. The Thomson modulator is based on the pulse step modulator (PSM) principle. This technology allows the regulation of the pulse voltage during the pulses and by this achieving a good flatness. In addition to the common PSM technology this modulator design includes additional features. The first one is a constant power regulation system in order to prevent a 10 Hz loading of the mains. The second one is the extension of a part of the system to allow 2-quadrant mode in order to demagnetise the core of the pulse transformer between the pulses. The modulator has been delivered to DESY in July 2008 and is under testing at the modulator test facility in Zeuthen. The paper will give a detailed overview on the system and shows the results of the factory testing and of the testing at DESY.
Effects of motor skills training and daily physical education: Research results in the Swedish Bunkeflo project (2014)

Effects of motor skills training and daily physical education : research results in the Swedish Bunkeflo project
Activation of T cells in vivo using anti-CD3 and staphylococcal enterotoxins. (1992)

One of the major problems in clinical immunity is that neoplastic and virally infected cells are insufficiently immunogenic to trigger an immune response. During the past several years, our laboratory has explored the use of T-cell-specific reagents including monoclonal antibodies (MAbs) and staphylococcal enterotoxins to amplify immune responses. This report summarizes our efforts to augment immunity with these agents.
Fretting Fatigue (1996)

Abstract Fretting is a special wear process that occurs at the contact area between two materials under load and subject to slight relative movement by vibration or some other force. This article focuses on measures to avoid or minimize crack initiation and fretting fatigue. It lists the factors that are known to influence the severity of fretting and discusses the variables that contribute to shear stresses. These variables include normal load, relative displacement (slip amplitude), and coefficient of friction. The article describes the general geometries and loading conditions for fretting fatigue. It presents the types of fretting fatigue tests and the effect of variables on fretting fatigue from different research test programs. The article also lists the general principles and practical methods for the abatement or elimination of fretting fatigue.
Permanent volume Bragg grating fabrication in pure lithium niobate crystal using direct laser writing technique (2011)

Transparent material modification with ultrafast laser pulses has attracted great scientific interest in the recent decade [1]. Depending on the laser pulse properties, homogeneous zones of modified refractive index can be implemented in dielectric material and with appropriate patterning algorithm, various photonic devices could be fabricated. Many different elements have been demonstrated in various types of glasses such as waveguides, diffractive gratings, microchannels and etc.
Generation of CD8 suppressor factor and β chemokines, induced by xenogeneic immunization, in the prevention of simian immunodeficiency virus infection in macaques (1998)

Previous xenogeneic immunization experiments in rhesus macaques with simian immunodeficiency virus (SIV) grown in human CD4(+) T cells consistently elicited protection from challenge with live SIV. However, the mechanism of protection has not been established. We present evidence that xenogeneic immunization induced significant CD8 suppressor factor, RANTES (regulated upon activation, normal T cell expressed and secreted), macrophage inflammatory protein (MIP) 1alpha, and MIP-1beta (P < 0.001 - P < 0.02). The concentrations of these increased significantly in protected as compared with infected macaques (P < 0.001). Xenogeneic stimulation in vitro also up-regulated CD8 suppressor factors (SF; P < 0.001) and the beta chemokines which were neutralized by antibodies to the 3 beta chemokines. Recombinant human RANTES, MIP-1alpha and MIP-1beta which bind to simian CCR5, suppressed SIV replication in a dose-dependent manner, with RANTES being more effective than the other two chemokines. The results suggest that immunization with SIV grown in human CD4(+) T cells induces CD8-suppressor factor, RANTES, MIP-1alpha and MIP-1beta which may block CCR5 receptors and prevent the virus from binding and fusion to CD4(+) cells.
Dimensions of the human adult mitral valve in the embalmed cadaver (2013)

Objectives: In human heart left atrioventricular orifice is guarded by mitral valve, which lies at the junction
of the left atrium and the left ventricle of the heart controlling and directing unidirectional blood flow during
ventricular diastole. Irrepairable damage to the mitral valve calls for its replacement using either a valve made
up of biological tissue or metal, pyrolytic carbon, vulcanised silicon rubber and similar materials. Prostheses
of these synthetic valve needs detailed knowledge of mitral orifice and valve dimensions. In the present study
an attempt was made to study the dimensions of mitral orifice and mitral valve in formalin fixed adult human
heart specimens. Methods: Mitral orifice was cut opened in these specimens and photographed with a scale.
This image was used for Scion image analysis for measurement of (i) Length of free margin and area of each
leaflet, (ii). Inner circumference of the mitral valve and (iii) Surface area of the mitral valve leaflets. Results: It
was found that circumference of annulus of mitral valve was 8-10 cm, length of free margin of anterior leaflet
was 5-7 cm, length of free margin of posterior leaflet was 7-9 cm, area of anterior leaflet was 1-3 cm2, and
area of posterior leaflet was 2-4 cm2. Conclusion: These data may be useful in designing the prosthetic valves.
Chemical synthesis and binding activity of the trypanosomatid cap-4 structure (2004)

Leishmania and other trypanosomatids are early eukaryotes that possess unusual molecular features, including polycistronic transcription and trans-splicing of pre-mRNAs. The spliced leader RNA (SL RNA) is joined to the 5' end of all mRNAs, thus donating a 5' cap that is characterized by complex modifications. In addition to the conserved m7GTP, linked via a 5'-5'-triphosphate bound to the first nucleoside of the mRNA, the trypanosomatid 5' cap includes 2'-O methylations on the first four ribose moieties and unique base methylations on the first adenine and the fourth uracil, resulting in the cap-4 structure, m7Gpppm3(6,6,2')Apm2'Apm2' Cpm2(3,2')U, as reported elsewhere previously. A library of analogs that mimic the cap structure to different degrees has been synthesized. Their differential affinities to the cap binding proteins make them attractive compounds for studying the molecular basis of cap recognition, and in turn, they may have potential therapeutic significance. The interactions between cap analogs and eIF4E, a cap-binding protein that plays a key role in initiation of translation, can be monitored by measuring intrinsic fluorescence quenching of the tryptophan residues. In the present communication we describe the multistep synthesis of the trypanosomatid cap-4 structure. The 5' terminal mRNA tetranucleotide fragment (pm3(6,6,2')Apm2'Apm2'Cpm2(3,2')U) was synthesized by the phosphoramidite solid phase method. After deprotection and purification, the 5'-phosphorylated tetranucleotide was chemically coupled with m7GDP to yield the cap-4 structure. Biological activity of this newly synthesized compound was confirmed in binding studies with eIF4E from Leishmania that we recently cloned (LeishIF4E-1), using the fluorescence time-synchronized titration method.
On the multiresolution enhancement of document images using fuzzy logic approach (2002)

Presents a method for enhancing document images that is based on the wavelet transform and fuzzy logic approach. The document image to be enhanced is obtained as a blurred binary image from a scanner and corrupted by additive noise. This type of image occurs in many situations when the quality of the documents including checks, hand-written text, credit card receipts become noisy and low-contrast images after scanning. Our problem is to improve such images by reducing noise and increasing the contrast of the text. This can be achieved by incorporating the wavelet transform, fuzzy logic and a contrast enhancement operator. The improvement is demonstrated by using simulation examples and compared in terms of visual enhancement with the more conventional approaches.
Analysis of human tear proteins by two‐dimensional electrophoresis (1992)

Human tear proteins in the conjunctival sac were separated on the basis of the differences in their isoelectric points and molecular weights using micro two-dimensional electrophoresis combined with immunoblotting. The two-dimensional electrophoretic patterns of tear proteins from patients with conjunctivitis were compared with those from normal individuals. We also measured integrated intensities of seven protein spots, lactoferrin (LF), albumin and five specific tear proteins (STP), to examine differences in the amounts of these proteins in tears from normal individuals of different sexes. In the tears from patients with conjunctivitis, secretory immunoglobulin A (IgA), LF and STP spots were stained more weakly, whereas the albumin spot was stained more strongly as compared with those from normal individuals. Furthermore, haptoglobin and IgG spots appeared in the tears from patients with conjunctivitis. These were more prominent in the tears from patients with severe conjunctivitis. There were significant differences in the amounts of LF and two kinds of STPs in the different sexes. The amounts of these proteins were larger in females.
연구논문(硏究論文) : 고스 하위문화 스타일의 미적 특성 (2013)

Subcultural style is the center of subcultural identity and the clothes and adornments are the most visible symbol of membership. The Goth subculture has associated tastes in music, aesthetics, and fashion. The style symbolized the strong subjective subcultural identity held by most Goths and acted as the practical basis to demonstrate their commitment to the subculture. This study investigates the aesthetic and the style of Goth subculture in its heyday of 1980s which has continued to affect mainstream fashion and culture since its birth. In order to inquire the concept of subculture and its style, this study executes literature survey as well as investigates the images of street style magazines to analyze the visual elements. The stylistic and the aesthetic characteristics of Goth subcultural style analyzed in this study are grouped into four categories as follows: first, Victoriana, which embodies the fear of death and the nostalgia for the past using Victorian morning dresses and corsets, second, vampirism, in that some Goths who are fascinated by vampires are costumed in vampire figures to advocate diabolism and decadence, third, sexual ambiguity of male Goths which emphasizes feminine appearance in the pursuit of androgyny, and fourth, sexual fetish of female Goths which represents aggressive eroticism utilizing fetish paraphernalia.
Dataflow-Functional High-Level Synthesis for Coarse-Grained Reconfigurable Accelerators (2018)

Domain-specific acceleration is now a must for all the computing spectrum, going from high performance computing to embedded systems. Unfortunately, system specialization is by nature a nightmare from the design productivity perspective. Nevertheless, in contexts where kernels to be accelerated are intrinsically streaming oriented, the combination of dataflow (DF) models of computation with coarse-grained reconfiguration (CGR) architectures can be particularly handful. In this letter we introduce a novel methodology to assemble and characterize virtually reconfigurable accelerators based on DF and functional programming principles, capable of addressing design productivity issues for CGR accelerators. The main advantage of the proposed methodology is accurate IP-level latency predictability improving design space exploration when compared with state-of-the-art high-level synthesis.
A Reflective, Processed-Kaolin Particle Film Affects Fruit Temperature, Radiation Reflection, and Solar Injury in Apple (2002)

Particle film technology is a new tool for tree fruit production systems. Trials were performed in Santiago, Chile, and Washington and West Virginia to evaluate the effect of particle film treatments on apple [ Malus sylvestris (L.) Mill var domestica (Borkh.) Mansf.] fruit temperature and the incidence of solar injury. Fruit surface temperature was reduced by the application of reflective particles and the amount of temperature reduction was proportional to the amount of particle residue on the fruit surface. Effective solar injury suppression was achieved with spray applications of 45 to 56 kg·ha -1 of a reflective, processed-kaolin particle film material in concentrations ranging from 3% to 12% in some of the locations. The timing of application to suppress solar injury was not clearly defined. The processed-kaolin particle film material was highly reflective to the ultraviolet wavelengths and this characteristic may be important in reducing solar injury to both fruit and leaves.
In Situ PCR techniques (1997)

Review of the PCR Technique. Preliminary Solution--Based Reactions. Preparation of Glass Slides and Tissues. In Situ PCR: DNA and RNA Targets. Special Applications of In Situ Amplification. Hybridization Reactions. Validation and Controls. Materials and Methods. Select Bibliography. Appendices. Index. About the Author.
Active Views for Electronic Commerce (1999)

Electronic commerce is emerging as a major Websupported application. In this paper we argue that database technology can, and should, provide the backbone for a wide range of such applications. More precisely, we present here the ActiveViews system, which, relying on an extensive use of database features including views, active rules (triggers), and enhanced mechanisms for notification, access control and logging/tracing of users activities, provides the needed basis for electronic commerce. Based on the emerging XML standards (DOM, query languages for XML, etc.), the system offers a novel declarative view specification language, describing the relevant data and activities of all actors (e.g. vendors and clients) participating in electronic commerce activities. Then, acting as an application generator, the system generates an actual, possibly customized, Web application that allows users to perform the given set of controlled activities and to work interactively on ...
Implications of sleep mode on activation and ranging protocols in PONs (2008)

A sleep mode protocol for ONUs is proposed and its impact on re-activation procedures for GPON is examined. The new protocol does not disrupt in-service ONUs.
The Rhetoric of Music in Dante’s Purgatorio (2009)

Dante's Purgatorio is the only canticle of his Divine Comedy in which souls develop.Those appearing in the Inferno undergo constant and unchanging torture, while those in the Paradiso spend eternity gazing at God, neither needing nor wishing for change.By contrast, the preparatory stage of Purgatory contains souls aimed entirely at development: from sin to spotlessness, from distracted and tainted desire to longing for God alone.Dante names Purgatory "that second realm / where man's soul goes to purify itself / and become worthy to ascend to Heaven" (Purgatorio 1.4-6).It is a stage of education, whose every aspect should accelerate souls toward their meeting with the Divine in the Paradiso.Spiritual exercises such as penance, benediction, and prayer contribute to this education, but another more subtle influence present throughout is music.Music is an expression of praise that would benefit any spiritual journey, but perhaps more important is its potential for beauty which, as Plato explains, has a profound effect on the soul.In beauty the soul gets a glimpse of the transcendent and begins to grow in its desire for the truth, goodness, and beauty of its ultimate goal, the Divine.In his dialogue The Phaedrus, Plato explains the power of beauty.It reminds the soul, he says, of the perfection of pure reality, and inspires it to "swel[l] up and ach[e]" with desire (Phaedrus 251c).Beauty, by introducing us to the transcendent, cultivates our longing to approach it further.It has the ability to sharpen the soul's desire for reality, making it an essential part of any truth-seeking education.
Application of Thermoelectric Modules Fabricated with (Bi,Sb)2(Te,Se)3 using Rapid Solidification Technique for the Projector System (2006)

A thermoelectric generator system was developed to convert low grade waste heat from a bulb inside an optical projection system into electricity. A new type of thermoelectric module with (Bi,Sb) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> (Te,Se) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> compounds and intended for consumer use was installed in the system. Bismuth-telluride-based thermoelectric compounds with fine grains and unidirectional crystal orientation prepared using a rapid solidification technique, exhibited high performance at temperatures ranging from 298 to 473 K. Thermoelectric properties were optimized by controlling the microstructure and oxygen content of the specimens. A maximum conversion efficiency of 5.7% was obtained from the developed thermoelectric module at a temperature differential of 150 K (Tc = 323 K, Th = 473 K). A thermoelectric power generating system using a new type of aluminum alloy reflector was developed and evaluated for the prototype projection system
Thermal decay in high density disk media (1998)

An experimental technique has been developed to accurately measure the time dependence of playback voltage due to the decay of recorded magnetization patterns. A "calibration" procedure is utilized to minimize the effect of magnetoresistive head sensitivity variations. This technique is applied to a systematic study of the dependence of the signal decay on recording bit density and magnetic layer thickness in longitudinal thin film media. A Neel-Arrhenius type model is introduced to calculate the degradation of a square wave magnetization pattern subject to thermal agitation. Because the equivalent external field which reduces the energy barrier is the time and spatially varying magnetostatic field produced by magnetic transitions, an iterative procedure is utilized to obtain the time dependent magnetization. The model takes into account distributions of grain volume and anisotropy axis. The calculation results are compared with the experimental data and good agreement is obtained.
Dis Marie c'était comment rue du Méridien 79 (1994)

info:eu-repo/semantics/published