gebhart/patrepeval huggingface dataset:
COI1 links jasmonate signalling and fertility to the SCF ubiquitin–ligase complex in <i>Arabidopsis</i> (2002)

Summary Jasmonates (JAs) regulate Arabidopsis thaliana wound and defence responses, pollen development, and stress‐related growth inhibition. Significantly, each of these responses requires COI1, an F‐box protein. Other F‐box proteins interact with SKP1 and cullin proteins to form SCF complexes that selectively recruit regulatory proteins targeted for ubiquitination. To determine whether COI1 also functions in an SCF complex, we have characterized Arabidopsis proteins that bind to COI1. An Arabidopsis cDNA expression library was screened in yeast for clones that produce proteins which can bind to COI1. We recovered two SKP1 homologues and a histone deacetylase. The Arabidopsis F‐box protein TIR1 interacted with SKP1 proteins, but not with the histone deacetylase. Mutant COI1 proteins revealed that the F‐box is required for interaction with SKP1s, but that sequences in leucine‐rich repeat domains are required for interaction with the histone deacetylase. Epitope‐tagged COI1 was introduced into Arabidopsis plants and cell cultures. Co‐immunoprecipitation experiments confirmed the interaction in planta of COI1 with SKP1‐like proteins and histone deacetylase, and also indicated that COI1 interacted with cullin. These results suggest that COI1 forms an SCF COI1 complex in vivo. COI1 is therefore expected to form a functional E3‐type ubiquitin ligase in plants and to regulate expression of jasmonate responsive genes, possibly by targeted ubiquitination of a histone deacetylase.
Evaluating the impact of job scheduling and power management on processor lifetime for chip multiprocessors (2009)

Temperature-induced reliability issues are among the major challenges for multicore architectures. Thermal hot spots and thermal cycles combine to degrade reliability. This research presents new reliability-aware job scheduling and power management approaches for chip multiprocessors. Accurate evaluation of these policies requires a novel simulation framework that can capture architecture-level effects over tens of seconds or longer, while also capturing thermal interactions among cores resulting from dynamic scheduling policies. Using this framework and a set of new thermal management policies, this work shows that techniques that offer similar performance, energy, and even peak temperature can differ significantly in their effects on the expected processor lifetime.
Kinetics of epidermis and adnexa following vitamin A acid in the human. (1976)

The effects of topically applied vitamin A acid and benzoyl peroxide were investigated. Short-term (a few days) and long-term (up to 800 days) treatment was analyzed histologically and autoradiographically. Labelled and unlabelled biopsies at various time intervals were taken from face, back, and acne lesions. Kinetics were followed with 3H-TdR and 3H-histidine. All keratinizing epithelia of epidermis and adnexa are stimulated by vitamin A acid. Labelling is high (up to 20-70% L.I) and occurs within a few days. Follicles and comedones can also be stimulated, but respond less and much later than epidermis. Transit time is shortened to 3-4 days. Lose, incoherent horny cells are produced, explaining the therapeutic benefits of vitamin A acid in acne. Benzoyl peroxide is less effective in stimulating epidermopoieses in epidermis, follicles, and comedones. This autoradiographic analysis parallels therapeutic results with these two exfoliants.
Smart Cities Mobility Monitoring through Automatic License Plate Recognition and Vehicle Discrimination (2021)

This work deals with vehicular monitoring within smart cities through Automatic License Plate Recognition (ALPR) techniques and vehicle discrimination object detection (YOLO), in order to obtain timely statistical data. The combined use of the two techniques allows to obtain much more refined data than a simple vehicle counter. Moreover, the collected data undergoes a process of anonymization in accordance with the European regulations for the protection of personal data (GDPR). The use of convolutional neural networks (CNN) made it possible to obtain vehicle tracking statistics, returning daily, weekly, monthly and yearly habits with the ultimate goal of allowing a monitoring and control of the city traffic conditions. The results obtained showed a high accuracy in the classification of vehicles and a wide range of statistics concerning the occurrences of each vehicle within the area of interest.
<i>Trypanosoma cruzi</i>highjacks TrkC to enter cardiomyocytes and cardiac fibroblasts while exploiting TrkA for cardioprotection against oxidative stress (2013)

Chronic Chagas cardiomyopathy (CCC), caused by the obligate intracellular protozoan parasite Trypanosoma cruzi, is a major cause of morbidity and mortality in Latin America. CCC begins when T. cruzi enters cardiac cells for intracellular multiplication and differentiation, a process that starts with recognition of host-cell entry receptors. However, the nature of these surface molecules and corresponding parasite counter-receptor(s) is poorly understood. Here we show that antibodies against neurotrophin (NT) receptor TrkC, but not against family members TrkA and TrkB, prevent T. cruzi from invading primary cultures of cardiomyocytes and cardiac fibroblasts. Invasion is also selectively blocked by the TrkC ligand NT-3, and by antagonists of Trk autophosphorylation and downstream signalling. Therefore, these results indicate that T. cruzi gets inside cardiomyocytes and cardiac fibroblasts by activating TrkC preferentially over TrkA. Accordingly, short hairpin RNA interference of TrkC (shTrkC), but not TrkA, selectively prevents T. cruzi from entering cardiac cells. Additionally, T. cruzi parasite-derived neurotrophic factor (PDNF)/trans-sialidase, a TrkC-binding protein, but not family member gp85, blocks entry dose-dependently, underscoring the specificity of PDNF as TrkC counter-receptor in cardiac cell invasion. In contrast to invasion, competitive and shRNA inhibition studies demonstrate that T. cruzi-PDNF recognition of TrkA, but not TrkC on primary cardiomyocytes and the cardiomyocyte cell line H9c2 protects the cells against oxidative stress. Thus, this study shows that T. cruzi via PDNF favours neurotrophin receptor TrkC for cardiac cell entry and TrkA for cardiomyocyte protection against oxidative stress, and suggests a new therapeutic opportunity in PDNF and/or fragments thereof for CCC therapy as entry inhibitors and/or cardioprotection agonists.
An immunity test method using electromagnetic wave of rotating polarization (2004)

An immunity/susceptibility test method using electromagnetic waves of rotating polarization is presented. Low-rate rotation of the field polarization is controlled electrically and continuously in a two-dimensional plane, so that radiated immunity/susceptibility characteristics depicted three-dimensionally can be obtained by using a turntable together with the method. This paper describes how to generate the rotating fields, and some experimentally measured characteristics of some examples. The results show the effectiveness of the proposed method to reveal the weak/strong immunity/susceptibility directions at a glance, so that it would be advantageous for trial phase of manufactured products.
Complete Generic-Level Phylogenetic Analyses of Palms (Arecaceae) with Comparisons of Supertree and Supermatrix Approaches (2009)

Supertree and supermatrix methods have great potential in the quest to build the tree of life and yet they remain controversial, with most workers opting for one approach or the other, but rarely both. Here, we employed both methods to construct phylogenetic trees of all genera of palms (Arecaceae/Palmae), an iconic angiosperm family of great economic importance. We assembled a supermatrix consisting of 16 partitions, comprising DNA sequence data, plastid restriction fragment length polymorphism data, and morphological data for all genera, from which a highly resolved and well-supported phylogenetic tree was built despite abundant missing data. To construct supertrees, we used variants of matrix representation with parsimony (MRP) analysis based on input trees generated directly from subsamples of the supermatrix. All supertrees were highly resolved. Standard MRP with bootstrap-weighted matrix elements performed most effectively in this case, generating trees with the greatest congruence with the supermatrix tree and fewest clades unsupported by any input tree. Nonindependence due to input trees based on combinations of data partitions was an acceptable trade-off for improvements in supertree performance. Irreversible MRP and the use of strictly independent input trees only provided no obvious benefits. Contrary to previous claims, we found that unsupported clades are not infrequent under some MRP implementations, with up to 13% of clades lacking support from any input tree in some irreversible MRP supertrees. To build a formal synthesis, we assessed the cross-corroboration between supermatrix trees and the variant supertrees using semistrict consensus, enumerating shared clades and compatible clades. The semistrict consensus of the supermatrix tree and the most congruent supertree contained 160 clades (of a maximum of 204), 137 of which were present in both trees. The relationships recovered by these trees strongly support the current phylogenetic classification of palms. We evaluate 2 composite supertree support measures (rQS and V) and conclude that it is more informative to report numbers of input trees that support or conflict with a given supertree clade. This study demonstrates that supertree and supermatrix methods can provide effective, explicit, and complimentary mechanisms for synthesizing disjointed phylogenetic evidence while emphasizing the need for further refinement of supertree methods.
Phase-Lock Loop Design for Coherent Angle-Error Detection in the Telstar Satellite Tracking System (1963)

The function of the angle-error detector is to provide pointing-error signals to the ground antenna control system, which allows operation in the autotrack mode once the satellite beacon has been acquired. The limitations on the accuracy of this system imposed by noise, phase jitter and Doppler effects are evaluated and the optimum design in terms of minimum mean-square error is developed. Design examples are given for both the horn-reflector antenna autotrack system and the precision tracker antenna system
Development of contactless measuring method of transient voltage on cable sheath (2002)

This paper proposes a contactless measuring method of a transient voltage on a cable sheath. The proposed method consists of quite simple elements, i.e. detecting and common electrodes and a lumped capacitor, based on capacitive coupling between the electrodes. The sheath voltage is measured as a dividing ratio between the sheath voltage and a voltage across the lumped detecting capacitor. The common electrode, covering the cable at a measuring part can reduce the effect of a stray capacitance. An experiment has been carried out on a scaled-down model of a cross-bonded cable. It has been confirmed from the experimental results that the proposed method has a sufficient accuracy.
Kinematics of Robot Fingers with Circular Rolling Contact Joints (2003)

Abstract This paper examines the kinematics of robot fingers that use RR subassemblies coupled by rolling contact to provide versatile one degree‐of‐freedom mechanical joints. A three degree‐of‐freedom robot finger constructed with these joints is a 6R planar chain with the R‐joints coupled together in pairs. The focus is on coupling based on pure rolling of circular cylinders which can be realized by friction contact, gearing, or cable tendons. We derive the forward, inverse and rate kinematics for this 3(RR) open chain. We then focus on the two degree‐of‐freedom 2(RR) case to illustrate the geometry of the system. An example design of a robot finger that incorporates these joints in order to provide compact movement is provided. © 2003 Wiley Periodicals, Inc.
The Home School (1982)

With regard to the editorial in the October/November 1981 issue of The Aboriginal Child at School concerning successful approaches to the education of Aboriginal children, I thought it may be of some interest to readers of the journal (especially those teachers transferring to schools serving significant numbers of Aboriginal students) to read about The Home School’s approach to education. This approach does not cater for any special needs of Aboriginal (and Islander) students – indeed all of its students are treated and taught in the same way – but for some reason Aboriginal children appear to be happy at this school, want to continue their education there next year and/or in years to come, and are generally motivated enough to achieve at least a pass level in most of their subjects. The Home School is a small, non-denominational, private, secondary boarding school and it has had fifteen students of Aboriginal descent enrolled as full boarders since 1978. They have mostly come from Aurukun, Laura and Mornington Island, and a grant from the Commonwealth Government covers their school fees. Eight of the 1981 Aboriginal students intend to return to this school in 1982, two of them to do Year 12 and four to do Year 11. All of these students are quite definite and enthusiastic about returning in 1982, which, in itself, is a success story, especially as many of them have been ‘cast-offs’ from the large state secondary schools in the local area. Either they had been truanting (in one case two girls had been at a local hostel for eight weeks before the hostel management found out that the girls had never attended the high school in which they had been enrolled) or they had been problem students and The Home School had been approached as a last chance for their successful education. (One boy who had been expelled from one of the local state secondary schools gave, a year later, The Home School’s major speech at the annual Speech Night, and a very impressive speech it is reported to have been.)
Determinants of Neutralization Resistance in the Envelope Glycoproteins of a Simian-Human Immunodeficiency Virus Passaged In Vivo (1999)

In vivo passage of a simian-human immunodeficiency virus (SHIV-89.6) generated a virus, SHIV-89.6P, that exhibited increased resistance to some neutralizing antibodies (G. B. Karlsson et al., J. Exp. Med. 188:1159-1171, 1998). Here we examine the range of human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies to which the passaged virus became resistant and identify envelope glycoprotein determinants of antibody resistance. Compared with the envelope glycoproteins derived from the parental SHIV-89.6, the envelope glycoproteins of the passaged virus were resistant to antibodies directed against the gp120 V3 variable loop and the CD4 binding site. By contrast, both viral envelope glycoproteins were equally sensitive to neutralization by two antibodies, 2G12 and 2F5, that recognize poorly immunogenic structures on gp120 and gp41, respectively. Changes in the V2 and V3 variable loops of gp120 were necessary and sufficient for full resistance to the IgG1b12 antibody, which is directed against the CD4 binding site. Changes in the V3 loop specified complete resistance to a V3 loop-directed antibody, while changes in the V1/V2 loops conferred partial resistance to this antibody. The epitopes of the neutralizing antibodies were not disrupted by the resistance-associated changes. These results indicate that in vivo selection occurs for HIV-1 envelope glycoproteins with variable loop conformations that restrict the access of antibodies to immunogenic neutralization epitopes.
MLH1 -93G>A Promoter Polymorphism and the Risk of Microsatellite-Unstable Colorectal Cancer (2007)

In two patient populations, the MLH1 -93G>A polymorphism was associated with an increased risk of MSI-H colorectal cancer.
PASA: Framework for partitioning and scheduling automation applications on multicore controllers (2014)

With multicore controllers becoming available for industrial automation applications, new tools and algorithms to compute efficient partitioning and scheduling solutions for control applications need to be developed. Optimizing the deployment and the schedule of a set of Function Block Diagrams on a parallel architecture are both NP hard. Additionally, control engineers need help to shift from the single core towards the multicore paradigm. By taking advantage of the parallelism inside the control applications it is effectively possible to decrease the finish times of the applications which enables to decrease their cycle times and improve the quality of service of the controller processes. This paper presents a practical solution to this problem that consists in a framework, called PASA, designed for partitioning and scheduling control applications modeled as function block diagrams. It enables new algorithms tailored to solve these optimization problems. This paper presents an extension of list-based DAG scheduling algorithms designed to compute a deployment and schedule for several control applications with different cycle times. The different variants of this algorithm are compared against each other as well as against some other existing solutions on a set of randomly generated examples.
The Indirect Hemagglutination Test for Malaria (1975)

Soluble antigens were prepared from Plasmodium falciparum and P. vivax and were evaluated in the indirect hemagglutination test. These antigens, attached to aldehyde-fixed type "O" erythrocytes, detected antibodies in more than 91% of infections with the homologous Plasmodium species. Detection rates in infections caused by the heterologous species ranged from 72% to 76%. Positive reactions occurred in less than 2% of sera from persons without malaria infection.
Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewis<sup>x</sup>and Lewis<sup>x</sup> (1999)

mAbs against tumor-associated carbohydrate antigens have the potential to play a prominent role in cancer immunotherapy. However, it has not been possible to fully exploit the clinical utility of such antibodies primarily, because those of adequate affinity could be derived only from murine sources. To address this problem, we prepared a single-chain Fv (scFv) antibody library from the peripheral blood lymphocytes of 20 patients with various cancer diseases. Completely human high-affinity scFv antibodies were then selected by using synthetic sialyl Lewisx and Lewisx BSA conjugates. These human scFv antibodies were specific for sialyl Lewisx and Lewisx, as demonstrated by ELISA, BIAcore, and flow cytometry binding to the cell surface of pancreatic adenocarcinoma cells. Nucleotide sequencing revealed that at least four unique scFv genes were obtained. The Kd values ranged from 1.1 to 6.2 x 10(-7) M that were comparable to the affinities of mAbs derived from the secondary immune response. These antibodies could be valuable reagents for probing the structure and function of carbohydrate antigens and in the treatment of human tumor diseases.
The Knoevenagel Reaction and the Synthesis of Unsaturated Nitro Compounds (1934)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Knoevenagel Reaction and the Synthesis of Unsaturated Nitro CompoundsDavid E. WorrallCite this: J. Am. Chem. Soc. 1934, 56, 7, 1556–1558Publication Date (Print):July 1, 1934Publication History Published online1 May 2002Published inissue 1 July 1934https://pubs.acs.org/doi/10.1021/ja01322a030https://doi.org/10.1021/ja01322a030research-articleACS PublicationsRequest reuse permissionsArticle Views744Altmetric-Citations20LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Pairings for Cryptographers. (2006)

Abstract. Many research papers in pairing based cryptography treat pairings as a “black box”. These papers build cryptographic schemes making use of various properties of pairings. If this approach is taken, then it is easy for authors to make invalid assumptions concerning the properties of pairings. The cryptographic schemes developed may not be realizable in practice, or may not be as efficient as the authors assume. The aim of this paper is to outline, in as simple a fashion as possible, the basic choices that are available when using pairings in cryptography. For each choice, the main properties and efficiency issues are summarized. The paper is intended to be of use to non-specialists who are interested in using pairings to design cryptographic schemes. 1
Nutrition, ageing and GFR: is age-associated decline inevitable? (1996)

Due to the paucity of long-term observational data, it is still unclear whether the decreased glomerular filtration rate (GFR) noted in older humans is a results of true changes in renal function over time. The fact that a carefully characterized subpopulation of subjects showed no decrease in GFR over time suggests that the 'physiological decrease' in GFR noted in the elderly is not inevitable. In studies in our patient population, there was a significant correlation between creatinine clearance and nutritional protein intake in elderly patients without renal disease. In our studies, elderly subjects without renal disease who ingested > 1 g/kg day of protein had creatinine clearances in the range of 90-100 ml/min/1.73 m2, while those with lower protein intakes had lower creatinine clearances. Our studies suggest that GFR is not a fixed function, and that its value may change both over short periods and over decades in humans, with these changes being associated with changes in nutritional protein intake. Low levels of GFR are not necessarily equivalent to a diagnosis of renal disease. Diet must be considered in the assessment of renal function in elderly patients before a diagnosis of renal insufficiency can be made. Decreased GFR is not an inevitable consequence of ageing in humans.
A real-time, large area, high space resolution particle radiography system (2014)

In this paper we describe a new detection system for the high resolution measurement of the residual range of charged particles, designed and developed with the aim of achieving real-time data acquisition and large detection areas. A prototype of the residual range detector, with a sensitive area of about 4 × 4 cm2, consisting of a stack of sixty ribbons of scintillating fibers (Sci-Fi) has been designed and tested. Each layer is read-out by two wavelength shifter (WLS) fibers and a position sensitive photomultiplier (PSPM). The Bragg peak shape is calculated real-time by the time over a suitable threshold for each channel. The results of the measurements taken using the prototype and a 62 MeV proton beam and a comparison with the GEANT4 simulations of the detector are presented. The main concepts on which the prototype is based have been used to demonstrate the technique patented by the INFN. The next step will be to design and validate the final detector which will have 30 × 30 cm2FOV and cover the 250 MeV proton range with about 150 micron range resolution. These performances are suitable for almost all medical imaging applications.