gebhart/patrepeval huggingface dataset:
Clinical trials with staphylococcal protein A. (1984)

Several reports have indicated antitumor effects from the immunoadsorption of plasma from tumor-bearing animals and humans with protein A-containing Staphylococcus aureus. The columns used for these treatments have varied widely in design. In a Phase I trial at the University of Washington, we used protein A chemically linked to crystalline silica for continuous immunoadsorption of plasma in 20 patients with advanced malignancies. Separated blood was perfused continuously over columns containing 50-100 g of immunoadsorbent with 100-200 mg protein A. One calculated plasma volume was perfused over the columns for each treatment. Patients received between 1 and 22 treatments, in total, at one to three treatments per week. Two patients had in vitro treatment of plasma separated from a unit of whole blood obtained by phlebotomy. Side effects were common, were usually manageable, and included chills and fever, nausea, tumor pain, hypotension, and respiratory symptoms. The observed antitumor responses were modest. Three of seven patients with melanoma had indications of responses less than 50%. One of three patients with breast cancer had a response less than 50%. The above studies demonstrate the feasibility of using staphylococcal protein A immunoadsorption in a larger group of patients with a more favorable disease stage.
Lack of mitochondrial and nuclear-encoded subunits of complex I and alteration of the respiratory chain in <i>Nicotiana sylvestris</i> mitochondrial deletion mutants (1997)

We previously have shown that Nicotiana sylvestris cytoplasmic male sterile (CMS) mutants I and II present large mtDNA deletions and that the NAD7 subunit of complex I (the main dehydrogenase of the mitochondrial respiratory chain) is absent in CMS I. Here, we show that, despite a large difference in size in the mtDNA deletion, CMS I and II display similar alterations. Both have an impaired development from germination to flowering, with partial male sterility that becomes complete under low light. Besides NAD7, two other complex I subunits are missing (NAD9 and the nucleus-encoded, 38-kDa subunit), identified on two-dimensional patterns of mitochondrial proteins. Mitochondria isolated from CMS leaves showed altered respiration. Although their succinate oxidation through complex II was close to that of the wild type, oxidation of glycine, a priority substrate of plant mitochondria, was significantly reduced. The remaining activity was much less sensitive to rotenone, indicating the breakdown of Complex I activity. Oxidation of exogenous NADH (coupled to proton gradient generation and partly sensitive to rotenone) was strongly increased. These results suggest respiratory compensation mechanisms involving additional NADH dehydrogenases to complex I. Finally, the capacity of the cyanide-resistant alternative oxidase pathway was enhanced in CMS, and higher amounts of enzyme were evidenced by immunodetection.
On the Security of Data Access Control for Multiauthority Cloud Storage Systems (2015)

Data access control has becoming a challenging issue in cloud storage systems. Some techniques have been proposed to achieve the secure data access control in a semitrusted cloud storage system. Recently, K. Yang et al. proposed a basic data access control scheme for multiauthority cloud storage system (DAC-MACS) and an extensive data access control scheme (EDAC-MACS). They claimed that the DAC-MACS could achieve efficient decryption and immediate revocation and the EDAC-MACS could also achieve these goals even though nonrevoked users reveal their Key Update Keys to the revoked user. However, through our cryptanalysis, the revocation security of both schemes cannot be guaranteed. In this paper, we first give two attacks on the two schemes. By the first attack, the revoked user can eavesdrop to obtain other users' Key Update Keys to update its Secret Key, and then it can obtain proper Token to decrypt any secret information as a nonrevoked user. In addition, by the second attack, the revoked user can intercept Ciphertext Update Key to retrieve its ability to decrypt any secret information as a nonrevoked user. Secondly, we propose a new extensive DAC-MACS scheme (NEDAC-MACS) to withstand the above two attacks so as to guarantee more secure attribute revocation. Then, formal cryptanalysis of NEDAC-MACS is presented to prove the security goals of the scheme. Finally, the performance comparison among NEDAC-MACS and related schemes is given to demonstrate that the performance of NEDAC-MACS is superior to that of DACC, and relatively same as that of DAC-MACS.
DNA Staining for Fluorescence and Laser Confocal Microscopy (1997)

We examined five nucleic acid binding fluorescent dyes, propidium iodide, SYBR Green I, YO-PRO-1, TOTO-3, and TO-PRO-3, for nuclear DNA staining, visualized by fluorescence and laser confocal microscopy. The optimal concentration, co-staining of RNA, and bleaching speeds were examined. SYBR Green I and TO-PRO-3 almost preferentially stained the nuclear DNA, and the other dyes co-stained the cytoplasmic RNA. RNAse treatment completely prevented the cytoplasmic RNA staining. In conventional fluorescence microscopy, these dyes can be used in combination with fluorescence-labeled antibodies. Among the dyes tested, TOTO-3 and TO-PRO-3 stained the DNAs with far-red fluorescence under red excitation. Under Kr/Ar-laser illumination, TOTO-3 and TO-PRO-3 were best suited as the nuclear staining dyes in the specimens immunolabeled with fluorescein and rhodamine (or Texas red).
Isolation of anti-colorectal tumour antibodies from a phage-display library (1997)

Conference Article| May 01 1997 Isolation of anti-colorectal tumour antibodies from a phage-display library KATHERINE P TOPPING; KATHERINE P TOPPING 1Academic Surgical Unit, University of Hull, Hull, HU6 7RX, UK Search for other works by this author on: This Site PubMed Google Scholar JOHN GREENMAN; JOHN GREENMAN 1Academic Surgical Unit, University of Hull, Hull, HU6 7RX, UK Search for other works by this author on: This Site PubMed Google Scholar JOHN R T MONSON JOHN R T MONSON 1Academic Surgical Unit, University of Hull, Hull, HU6 7RX, UK Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1997) 25 (2): 268S. https://doi.org/10.1042/bst025268s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation KATHERINE P TOPPING, JOHN GREENMAN, JOHN R T MONSON; Isolation of anti-colorectal tumour antibodies from a phage-display library. Biochem Soc Trans 1 May 1997; 25 (2): 268S. doi: https://doi.org/10.1042/bst025268s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1997 Biochemical Society1997 Article PDF first page preview Close Modal You do not currently have access to this content.
Achilles Tendon Rupture (2009)

Sports participation has undergone an increase in recent decades. Injury due to sporting activity has also recently risen. The Achilles tendon has been one of the most common sports-related injuries. A 2 in 100,000 individual Achilles tendon injury rate increased to a 12 in 100,000 individual injury rate in less than 10 years. The injury is typically observed in men in the fourth to fifth decades of life. Male to female injury ratios range from 2:1 to 12:1. Running, jumping, and agility activities involving eccentric loading and explosive plyometric contractions are usual mechanisms. Natural aging allows predisposing chronic degeneration of the tendon. Blood flow decreases and stiffness increases with aging to decrease the ability to withstand stress. Noninflammatory tendinosis and chronic tendinopathy are 2 separate processes proposed for tendon degeneration and subsequent rupture. Rupture typically occurs 2 to 6 cm proximal to the calcaneal insertion. Predisposing factors are grouped into 2 categories: intrinsic and extrinsic risk factors. Avoidance of degenerative changes within the tendon is the primary method to prevent rupture. Regular physical activity as athletes age also promotes tendon hypertrophy, increases nutrient delivery, and reduces collagen fiber fatigue.
Synthetic Antenna Functioning As Light Harvester in the Whole Visible Region for Enhanced Hybrid Photosynthetic Reaction Centers (2016)

The photosynthetic reaction center (RC) from the Rhodobacter sphaeroides bacterium has been covalently bioconjugated with a NIR-emitting fluorophore (AE800) whose synthesis was specifically tailored to act as artificial antenna harvesting light in the entire visible region. AE800 has a broad absorption spectrum with peaks centered in the absorption gaps of the RC and its emission overlaps the most intense RC absorption bands, ensuring a consistent increase of the protein optical cross section. The covalent hybrid AE800-RC is stable and fully functional. The energy collected by the artificial antenna is transferred to the protein via FRET mechanism, and the hybrid system outperforms by a noteworthy 30% the overall photochemical activity of the native protein under the entire range of visible light. This improvement in the optical characteristic of the photoenzyme demonstrates the effectiveness of the bioconjugation approach as a suitable route to new biohybrid materials for energy conversion, photocatalysis, and biosensing.
Laboratory simulation studies of the recycle oil pyrolysis and extraction concept (1989)

The Western Research Institute is currently developing a process for the recovery of distillable liquid products from alternate fossil fuel sources such as tar sand and oil shale. The processing concept is based on recycling a fraction of the product oil back into the reactor with the raw resource. This concept is termed the recycle oil pyrolysis and extraction (ROPE {copyright}) process. The conversion of the solid resource to a liquid fuel is performed in two stages. The first recovery stage is performed at moderate temperatures (325--420{degree}C (617--778{degree}F)) in the presence of product oil recycle. The second stage is performed at higher temperatures (450--540{degree}C (842--1004{degree}F)) in the absence of product oil recycle. The experiments were performed using Asphalt Ridge tar sand in an all-glass reactor at the bench-scale level and simulated three of the five conceptual processing zones. The three zones that were simulated include (1) the tar sand preheat zone, (2) the recycle oil pyrolysis, and (3) the recycle oil reflux zone. The evaluation of the data from these experiments provide a description of the processes that are occurring in each zone. 4 refs., 6 figs., 29 tabs.
Structural health and prognostics management for offshore wind turbines : (2013)

Operations and maintenance costs for offshore wind plants are significantly higher than the current costs for land-based (onshore) wind plants. One way to reduce these costs would be to implement a structural health and prognostic management (SHPM) system as part of a condition based maintenance paradigm with smart load management and utilize a state-based cost model to assess the economics associated with use of the SHPM system. To facilitate the development of such a system a multi-scale modeling approach developed in prior work is used to identify how the underlying physics of the system are affected by the presence of damage and faults, and how these changes manifest themselves in the operational response of a full turbine. This methodology was used to investigate two case studies: (1) the effects of rotor imbalance due to pitch error (aerodynamic imbalance) and mass imbalance and (2) disbond of the shear web; both on a 5-MW offshore wind turbine in the present report. Based on simulations of damage in the turbine model, the operational measurements that demonstrated the highest sensitivity to the damage/faults were the blade tip accelerations and local pitching moments for both imbalance and shear web disbond. The initial cost model provided a great deal of insight into the estimated savings in operations and maintenance costs due to the implementation of an effective SHPM system. The integration of the health monitoring information and O&M cost versus damage/fault severity information provides the initial steps to identify processes to reduce operations and maintenance costs for an offshore wind farm while increasing turbine availability, revenue, and overall profit.
El cerebro adicto (2017)

Cada vez sabemos mas acerca de esa compulsion que nos aboca a unos habitos autodestructivos y sobre como la ciencia podria ayudarnos a combatirla
Coulomb Barrier for Charge Separation at an Organic Semiconductor Interface (2008)

Charge transfer (CT) excitons across donor-acceptor interfaces are believed to be barriers to charge separation in organic solar cells, but little is known about their physical characteristics. Here, we probe CT excitons on a crystalline pentacene surface using time-resolved two-photon photoemission spectroscopy. CT excitons of 1s, 2s, and 3s characters are bound by Coulomb energies of 0.43, 0.21, 0.12 eV, respectively, in agreement with quantum mechanical modeling. The large binding energy of the 1s CT exciton excludes its participation in photovoltaics. Efficient charge separation in organic heterojunction solar cells must involve a series of hot CT excitons.
Effect of Volume Concentration Ratio of Cell - free Medium on Tetramethylpyrazine Production by Lactococcus lactis subsp . lactis biovar . diacetilactis FC1 (1994)

Acetoin and ammonia, the precursors of tetramethylpyrazine (TMP) having nutty or roasted flavors, were produced by cultivating Lactococcus lactis ssp. lactis biovar. diacetilactis FC1. The effects of the volume concentration ratio (VCR) of cell-free medium on the formation of TMP were investigated using a rotary evaporator at . As the VCR increased, the formation of TMP and the conversion ratio of acetoin to TMP increased. More TMP were formed at . As the VCR increased, the concentration of acetoin decreased implying the formation of TMP from acetoin and ammonia.
High Carbon Dioxide Atmospheres Affect Stored 'Thompson Seedless' Table Grapes (2002)

Efficacy of controlled atmosphere (CA) conditions for decay control in 'Thompson Seedless' table grapes was evaluated during the 1998-2000 seasons. During the 1998 season, early (16.5% soluble solids concentration = SSC) and late harvested (19% SSC) grapes were exposed to 5%, 10%, 15%, 20%, or 25% CO 2 combined with 3%, 6%, and 12% O 2 . In 1999 and 2000, 10% or 15% CO 2 combined with 3%, 6%, or 12% O 2 were used. In all trials, fruit were initially SO 2 fumigated and air-stored grapes were used as controls. Storage atmospheres did not affect SSC, titratable acidity (TA), or sugar-to-acid ratio (SSC: TA). The main storage limitations for early harvested 'Thompson Seedless' table grapes were “off flavor” and rachis and berry browning development, which resulted from exposure to >10% CO 2 . However, ≥15% CO 2 was needed to control total decay and nesting development independent of O 2 concentrations. High carbon dioxide atmospheres (15% to 25%) were more effective in decay control without detrimental effects on quality when late harvested grapes were used. The combination of 15% CO 2 with 3%, 6%, or 12% O 2 is suggested for up to 3 months storage only for late harvested 'Thompson Seedless' table grapes; it should not be used for early harvested grapes.
Antiangiogenic therapy: a novel approach to overcome tumor hypoxia (2010)

Hypoxia is a common phenomenon in solid tumors. Resistance of hypoxic tumor cells to radiation is a significant reason of failure in the local control of tumors. The growth and metastasis of solid tumors rely on blood vessels. Antiangiogenic agents mainly target tumor blood vessels, and radiation therapy mainly targets tumor cells. Combination of antiangiogenic treatment and radiation exhibits synergistic effect, which improves the response of tumors to radiation therapy. The mechanisms of interaction between antiangiogenic agents and ionizing radiation are complex and involve interactions between tumor cells and tumor microenvironment, including tumor oxygenation, stroma, and vasculature. The original mechanism of antiangiogenesis is to induce ischemia and hypoxia in tumors, thereby, "starve" the tumors. However, recently, emerging data suggest that antiangiogenic agents could reduce the proportion of hypoxic cells through normalizing tumor vasculature, decreasing oxygen consumption, and other mechanisms. The use of antiangiogenic agents provides a new approach to overcome the hypoxia problem, and ultimately improves the efficacy of radiation therapy. In this review, we discuss tumor hypoxia, tumor angiogenesis and its regulation, mechanisms of antiangiogenic therapy combined with radiation therapy, and how antiangiogenic therapy overcomes tumor hypoxia.
Membrane protein structure determination — The next generation (2013)

The field of Membrane Protein Structural Biology has grown significantly since its first landmark in 1985 with the first three-dimensional atomic resolution structure of a membrane protein. Nearly twenty-six years later, the crystal structure of the beta2 adrenergic receptor in complex with G protein has contributed to another landmark in the field leading to the 2012 Nobel Prize in Chemistry. At present, more than 350 unique membrane protein structures solved by X-ray crystallography (http://blanco.biomol.uci.edu/mpstruc/exp/list, Stephen White Lab at UC Irvine) are available in the Protein Data Bank. The advent of genomics and proteomics initiatives combined with high-throughput technologies, such as automation, miniaturization, integration and third-generation synchrotrons, has enhanced membrane protein structure determination rate. X-ray crystallography is still the only method capable of providing detailed information on how ligands, cofactors, and ions interact with proteins, and is therefore a powerful tool in biochemistry and drug discovery. Yet the growth of membrane protein crystals suitable for X-ray diffraction studies amazingly remains a fine art and a major bottleneck in the field. It is often necessary to apply as many innovative approaches as possible. In this review we draw attention to the latest methods and strategies for the production of suitable crystals for membrane protein structure determination. In addition we also highlight the impact that third-generation synchrotron radiation has made in the field, summarizing the latest strategies used at synchrotron beamlines for screening and data collection from such demanding crystals. This article is part of a Special Issue entitled: Structural and biophysical characterisation of membrane protein-ligand binding.
Direct synthesis and characterization of crosslinked polysiloxanes via anionic ring‐opening copolymerization with octaisobutyl‐polyhedral oligomeric silsesquioxane and octamethylcyclotetrasiloxane (2006)

Abstract The crosslinked polysiloxanes were directly synthesized by anionic ring‐opening copolymerization of octaisobutyl‐polyhedral oligomeric silsesquioxane (POSS) as a multifunctional monomer with octamethylcyclotetrasiloxane (D 4 ) under base catalysts such as potassium hydroxide (KOH) or tetramethylammonium hydroxide (Me 4 NOH) siloxanolate. The mechanism of anionic ring‐opening copolymerization of octaisobutyl‐POSS and D 4 was discussed and the influences of the polar additive N , N ‐dimethylacetamide on gelation time at different temperatures were investigated. The results of gel content and swelling ratio, GPC, solid‐state 29 Si and 13 C NMR, FTIR, XRD show that octaisobutyl‐POSS is reacted and most of the product is crosslinked. The DSC and TG results indicate that the crosslinked polysiloxanes exhibit distinct glass transition temperatures ( T g ) and excellent thermal stability. Compared to that under KOH siloxanolate, the crosslinked polysiloxane synthesized with Me 4 NOH siloxanolate has better preferable thermal stability. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 3848–3856, 2006
A direct matrix approach to 3-D antenna radiation-pattern estimation from partially-scanned spherical near-field data (2011)

Far-field radiation-pattern estimation is explored for the case of partially-scanned spherical near-field data resulting from physical limitations of an antenna under test or associated measurement systems. A direct matrix approach, based on spherical-wave expansions, is found to be simple and effective for constructing the 3-D radiation pattern from partially-scanned near-field data. Criteria to determine the minimum near-field data for full 3-D pattern reconstruction is explained. The concept of the direct matrix approach is demonstrated for a mobile antenna and a horn antenna. An additional benefit of this approach is the reduction of the scan time for antenna measurements.
PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity. (1992)

PU.1 is a B-cell- and macrophage-specific transcription factor. By an electrophoretic mobility shift assay and dimethyl sulfate methylation interference assays, we show that PU.1 binds to DNA sequences within the immunoglobulin kappa 3' enhancer (kappa E3'). Binding of PU.1 to the kappa E3' enhancer assists the binding of a second tissue-restricted factor, NF-EM5, to an adjacent site. Binding of NF-EM5 to kappa E3' DNA sequences requires protein-protein interaction with PU.1 as well as specific protein-DNA interactions. This is the first known instance of PU.1 interacting with another cellular protein. NF-EM5 does not cofractionate with PU.1, suggesting that it is a distinct protein and is not a posttranslational modification of PU.1. UV-crosslinking studies and elution from sodium dodecyl sulfate-polyacrylamide gels indicate that NF-EM5 is a protein of approximately 46 kDa. Site-directed mutagenesis studies of the PU.1- and EM5-binding sites indicate that these sites play important roles in kappa E3' enhancer activity. By using a series of PU.1 deletion constructs, we have identified a region in PU.1 that is necessary for interaction with NF-EM5. This segment encompasses a 43-amino-acid region with PEST sequence homology, i.e., one that is rich in proline (P), glutamic acid (E), serine (S), and threonine (T).
Miniature Rectifier Computing and Controlling Circuits (1952)

General types of rectifier computing circuits are described and analyzed. Stress is placed upon the realization of the highest speed of operation. Selenium-rectifier elements are used throughout, although the same analysis applies to all other types of rectifiers. A speed of 100 kc obtlainable from selenium rectifiers. Megacycle operation is possible. Special techniques of circuit construction are developed which result in a simple, compact, and economical system, applicable not only to large-scale digital computing machines but also to complicated control systems.