gebhart/patrepeval huggingface dataset:
Identification of a 2 base pair nonsense mutation causing a cryptic splice site in a DMD patient (1992)

Journal Article Identification of a 2 base pair nonsense mutation causing a cryptic splice site in a DMD patient Get access A.V. Winnard, A.V. Winnard 2Department of Medical Biochemistry, College of Medicine, The Ohio State UniversityColumbus, OH Search for other works by this author on: Oxford Academic PubMed Google Scholar Y. Jia-Hsu, Y. Jia-Hsu 4Institute for Molecular Genetics, Howard Hughes Medical Institute, Baylor College of MedicineHouston, TX, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar R.A. Gibbs, R.A. Gibbs 4Institute for Molecular Genetics, Howard Hughes Medical Institute, Baylor College of MedicineHouston, TX, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar J.R. Mendell, J.R. Mendell 1Department of Neurology, The Ohio State UniversityColumbus, OH Search for other works by this author on: Oxford Academic PubMed Google Scholar A.H.M. Burghes A.H.M. Burghes * 1Department of Neurology, The Ohio State UniversityColumbus, OH2Department of Medical Biochemistry, College of Medicine, The Ohio State UniversityColumbus, OH3Department of Molecular Genetics, College of Biological Sciences, The Ohio State UniversityColumbus, OH *To whom corrspondence should be addressed at: Department of Neurology, 452 Means Hall, 1654 Upham Drive, The Ohio State University, Columbus, OH 43210, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Human Molecular Genetics, Volume 1, Issue 8, October 1992, Pages 645–646, https://doi.org/10.1093/hmg/1.8.645 Published: 01 October 1992 Article history Received: 27 July 1992 Revision received: 10 September 1992 Accepted: 10 September 1992 Published: 01 October 1992
A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm (2005)

Thrombin-inhibiting DNA aptamers have already been obtained through the systematic evolution of ligands by exponential enrichment (SELEX). However, SELEX is a method that screens DNA aptamers that bind to their target molecules, and it sometimes fails to screen good inhibitors. Therefore, it is necessary to develop a method of screening DNA aptamers based on their inhibitory effects on the target molecules. We developed a novel method of detecting aptamers using an evolution-mimicking algorithm, and we applied it to the search of new aptamers which inhibit thrombin. First, we randomly designed and synthesized ten 15mer oligonucleotides presumed to form G-quartet structures, and then measured their thrombin-inhibiting activities. The aptamers showing high inhibitory activity were selected, and we shuffled and mutated those sequences in silico to generate 10 new sequences of next-generation aptamers. After repeating the cycle five times, we successfully obtained the same aptamers reported previously, and they showed high inhibitory activity. In addition, we added 8mer oligonucleotides to both the 5' and the 3' end of the selected 15mer aptamers, and then repeated the evolution in silico. After two cycles, we were able to obtain aptamers with higher inhibitory activity than that of the 15mer aptamers.
MicroRNA-99a-5p in circulating immune cells as a potential biomarker for the early diagnosis of ischemic stroke (2017)

This study demonstrated that miR-99a-5p levels in circulating immune cells might facilitate the diagnosis of ischemic stroke.
The global burden and impact of chronic obstructive pulmonary disease worldwide. (2001)

A prerequisite for fighting chronic obstructive pulmonary disease (COPD) world-wide is accurate data on death and illness. Such data are not available in many countries and surveys with spirometry of forced expiratory volumes should be performed world-wide. Severe COPD, with one second FEV1 below 40% of predicted value, has been estimated to be below 1% in the general population. Mild to moderate COPD can be 10 times more frequent. The prevalence of COPD increases heavily with age. The Global Burden of Disease study, undertaken by the World Bank and World Health Organisation, concluded that COPD worldwide will increase from 1990 to 2020 its rank number of death from rank 6 to rank 3, and its rank number of disability-adjusted life years lost from rank 12 to rank 5. Reduction of total personal exposure to tobacco smoke and occupational and other indoor or outdoor dusts are important goals to prevent the onset and progression of COPD.
An Improved Deep Ultra Violet (DUV) Multilayer Resist Process For High Resolution Lithography (1984)

An improved DUV multi-layer resist process is developed by using a spun-on anti-reflective coating (ARC) that has been used between the top layer resist and PMMA. By using this structure, significant advantages over the basic structure can be realized such as an elimination of interface mixture, elimination of surface reflection problems, no degradation of PMMA sensitivity, and it also eliminates the resist transmission peak at 250nm so that marginal resists such as AZ2400 and K-820 can be used. By using this improved resist structure and a developer that retains the top layer resist, plasma etching capabilities have been demonstrated on Si0<sub>2</sub>, polysilicon and aluminum. Due to the planarization effect of PMMA and elimination of surface reflection, excellent pattern fidelity can be obtained down to submicron features. This improved bi-layer resist process provides a low cost, reliable method to extend the capability of current generation optical lithographic equipments. This improved process was used for high resolution device fabrication including contact holes and aluminum metallization.
Cardiac-specific attenuation of natriuretic peptide A receptor activity accentuates adverse cardiac remodeling and mortality in response to pressure overload (2005)

Atrial (ANP) and brain (BNP) natriuretic peptides are hormones of myocardial cell origin. These hormones bind to the natriuretic peptide A receptor (NPRA) throughout the body, stimulating cGMP production and playing a key role in blood pressure control. Because NPRA receptors are present on cardiomyocytes, we hypothesized that natriuretic peptides may have direct autocrine or paracrine effects on cardiomyocytes or adjacent cardiac cells. Because both natriuretic peptides and NPRA gene expression are upregulated in states of pressure overload, we speculated that the effects of the natriuretic peptides on cardiac structure and function would be most apparent after pressure overload. To attenuate cardiomyocyte NPRA activity, transgenic mice with cardiac specific expression of a dominant-negative (DN-NPRA) mutation (HCAT D 893A) in the NPRA receptor were created. Cardiac structure and function were assessed (avertin anesthesia) in the absence and presence of pressure overload produced by suprarenal aortic banding. In the absence of pressure overload, basal and BNP-stimulated guanylyl cyclase activity assessed in cardiac membrane fractions was reduced. However, systolic blood pressure, myocardial cGMP, log plasma ANP levels, and ventricular structure and function were similar in wild-type (WT-NPRA) and DN-NPRA mice. In the presence of pressure overload, myocardial cGMP levels were reduced, and ventricular hypertrophy, fibrosis, filling pressures, and mortality were increased in DN-NPRA compared with WT-NPRA mice. In addition to their hormonal effects, endogenous natriuretic peptides exert physiologically relevant autocrine and paracrine effects via cardiomyocyte NPRA receptors to modulate cardiac hypertrophy and fibrosis in response to pressure overload.
Solvent‐Stimulated Luminescence from the Supramolecular Aggregation of a Trinuclear Gold(<scp>I</scp>) Complex that Displays Extensive Intermolecular AuċAu Interactions (1997)

Striking yellow luminescence is observed upon addition of a drop of solvent to colorless crystals of [Au3(CH3NCOCH3)3] that were previously irradiated with near-visible light. The trigonal prismatic stacking of individual molecules of the triangular cluster in the solid state (shown on the right) sheds light on the possible mechanisms for this luminescence.
Analytical approach to grey levels in ferroelectric liquid crystal displays (2001)

Grey levels in ferroelectric liquid crystal displays can be obtained through 'continuous director rotation' in monostable modes. Displays can only be actively addressed. A very simple description of this continuous director rotation can be given in the so-called uniform φ theory, where it is assumed that the azimuthal angle φ of the director does not vary throughout the thickness of the liquid crystal. It requires however that the equilibrium state at zero voltage corresponds to the director making a maximum angle, i.e. the cone angle, with the glass substrates, the polarization being parallel to the glass substrates. It is shown that the electrooptic response can be given an analytic treatment which reduces to the uniform φ theory with the appropriate zero voltage equilibrium state. It accounts entirely for the observed V-shaped switching in high-P materials. In particular an exact expression for the saturation voltage and its dependence on the P value and on the alignment layer thickness is given.
The Effect of Dynamic Copies Model in Teaching Recursive Programming (1998)

The copies model of recursion was implemented in two versions of computer-based instruction (dynamic vs. static) in this study. For the immediate effects, dynamic copies model was more effective than static copies model in teaching recursion. High prior knowledge students performed better than low prior knowledge students no matter they were instructed with the dynamic or static copies model. For the delayed effects, ATI was found. High prior knowledge students benefited from the static copies model instruction more than from the dynamic copies model. In contrast, low prior knowledge students benefited from the dynamic copies model more than from the static copies model.
Monogenic Human Obesity Syndromes (2004)

Over the past decade, we have witnessed a major increase in the scale of scientific activity devoted to the study of energy balance and obesity. This explosion of interest has, to a large extent, been driven by the identification of genes responsible for murine obesity syndromes and the novel physiological pathways revealed by those genetic discoveries. We and others recently have identified several single-gene defects causing severe human obesity. Many of these defects have occurred in molecules identical or similar to those identified as a cause of obesity in rodents. This chapter will consider the human monogenic obesity syndromes that have been characterized to date and discuss how far such observations support the physiological role of these molecules in the regulation of human body weight and neuroendocrine function.
Special Compensation for Salaried Chemists and Rewards for Inventors (1977)

Special compensation for the employed inventor has been mandated by statute for many years in a number of countries foreign to the United States. In this country such compensation, if any, is customarily provided by individual employers at their discretion. While a bill to provide mandatory compensation has been introduced with modifications by Rep. Moss of California at each session of Congress since 1970, no action has yet been taken. During this period the ACS Committee on Patent Matters and Related Legislation, jointly with the Economic Status Committee, has been studying the various provisions of the bill in order to adopt an official ACS position on it. The relationships between this bill and existing legislation in other countries are discussed. Proposals and procedures other than legislative are outlined for on-going discussion.
Omega-3 fatty acids improve the diagnosis-related clinical outcome* (2006)

Administration of omega-3 fatty acid may reduce mortality, antibiotic use, and length of hospital stay in different diseases. Effects and effect sizes related to fish oil doses are diagnosis dependent. In view of the lack of substantial study literature concerning diagnosis-related nutritional single-substrate intervention in the critically ill, the present data can be used in formulating hypotheses and may serve as reference doses for randomized, controlled studies, which may, for instance, confirm the value of omega-3 fatty acid in the adjunctive therapy of peritonitis and abdominal sepsis.
Clipping error rate-adaptive color compensation based on gamut extension for a low-performance camera in a projector-camera system (2011)

As projectors become smaller and less expensive, the use of mobile (or portable) projectors is significantly increasing in environments where an ideal white screen is not readily available. Images from mobile projectors in this environment are often reproduced with visually distracting color distortion due to the use of arbitrary colored projection surfaces. In this paper, we propose an adaptive color compensation method that compensates for color distortion occurring in images projected onto an arbitrary colored projection surface without requiring use of a high-performance camera in a projector-camera system. The proposed method is based on gamut extension of a low-performance camera for device characterization and uses an adaptive clipping error reduction algorithm. Experimental results show that the proposed method effectively compensates for color distortion with an affordable low-performance camera, making the projector-camera system far more practical and marketable.
Minority report in fraud detection (2004)

This paper proposes an innovative fraud detection method, built upon existing fraud detection research and Minority Report , to deal with the data mining problem of skewed data distributions. This method uses backpropagation (BP), together with naive Bayesian (NB) and C4.5 algorithms, on data partitions derived from minority oversampling with replacement. Its originality lies in the use of a single meta-classifier (stacking) to choose the best base classifiers, and then combine these base classifiers' predictions (bagging) to improve cost savings (stacking-bagging). Results from a publicly available automobile insurance fraud detection data set demonstrate that stacking-bagging performs slightly better than the best performing bagged algorithm, C4.5, and its best classifier, C4.5 (2), in terms of cost savings. Stacking-bagging also outperforms the common technique used in industry (BP without both sampling and partitioning). Subsequently, this paper compares the new fraud detection method (meta-learning approach) against C4.5 trained using undersampling, oversampling, and SMOTEing without partitioning (sampling approach). Results show that, given a fixed decision threshold and cost matrix, the partitioning and multiple algorithms approach achieves marginally higher cost savings than varying the entire training data set with different class distributions. The most interesting find is confirming that the combination of classifiers to produce the best cost savings has its contributions from all three algorithms.
The Preparation and Some Properties of Crystalline Glucose 6-Phosphate Dehydrogenase from Leuconostoc mesenteroides<sup>*</sup> (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Preparation and Some Properties of Crystalline Glucose 6-Phosphate Dehydrogenase from Leuconostoc mesenteroides*Charles Olive and H. Richard LevyCite this: Biochemistry 1967, 6, 3, 730–736Publication Date (Print):March 1, 1967Publication History Published online1 May 2002Published inissue 1 March 1967https://pubs.acs.org/doi/10.1021/bi00855a012https://doi.org/10.1021/bi00855a012research-articleACS PublicationsRequest reuse permissionsArticle Views208Altmetric-Citations76LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Fibrin Gel-Immobilized Primary Osteoblasts in Calcium Phosphate Bone Cement: In vivo Evaluation with Regard to Application as Injectable Biological Bone Substitute (2005)

Osteogenic injectable bone substitutes may be useful for many applications. We developed a novel injectable bone substitute based on osteoblast-fibrin glue suspension and calcium phosphate bone cement (BC). Human osteoblasts were isolated from trabecular bone samples and cultured under standard conditions. Osteoblasts were suspended in fibrinogen solution (FS). BC was cured with thrombin solution. 8 x 4 mm injectable bone discs were prepared using silicon molds and a custom-made applicator device. Discs containing BC, BC/FS, or BC/FS/osteoblasts were implanted subcutaneously into athymic nude mice. After 3, 9 and 24 weeks, specimens were explanted and subjected to morphologic and biomechanical evaluation. In vitro fibrin gel-embedded osteoblasts displayed a differentiated phenotype as evidenced by alkaline phosphatase, collagen type 1 and von Kossa stains. A proportion of osteoblasts appeared morphologically intact over a 3-day in vitro period following application into the BC. BC/FS and BC/FS/osteoblast discs were sparsely infiltrated with vascularized connective tissue. There was no bone formation in implants from all groups. However, positive von Kossa staining only in BC/FS/osteoblast groups suggests engraftment of at least some of the transplanted cells. Biomechanical evaluation demonstrated initial stability of the composites. Young's modulus and maximal load did not differ significantly in the BC/FS and BC/FS/osteoblast groups. The practicability of osteoblast-containing injectable bone could be demonstrated. The dense microstructure and the suboptimal initial vascularization of the composites may explain the lack of bone formation. Modifications with regard to enhanced osteoblast survival are mandatory for a possible application as injectable osteogenic bone replacement system.
Targeting of PML/RAR Is Lethal to Retinoic Acid–Resistant Promyelocytic Leukemia Cells (1998)

Acute promyelocytic leukemia (APL) cells, containing the t(15;17) rearrangement, express the fusion protein, PML/RAR. Clinically, patients respond to all-trans retinoic acid (ATRA) through complete remissions associated with myeloid maturation of leukemic cells. This clinical ATRA response of APL is linked to PML/RAR expression. Unfortunately, these remissions are transient and relapsed APL is often ATRA-resistant. The role PML/RAR plays in the growth and maturation of these APL cells with acquired ATRA resistance has not been fully explored. This study uses an ATRA-resistant NB4 cell line (NB4-R1) to investigate the contribution of PML/RAR expression to ATRA resistance. Targeting of PML/RAR in NB4-R1 cells was undertaken using two approaches: homologous recombination and hammerhead ribozyme-mediated cleavage. Reducing PML/RAR protein in NB4-R1 cells rendered these cells more sensitive to ATRA. These cells were growth-inhibited in ATRA, apoptosis was induced, and there was no apparent signaling of differentiation. Sequence analysis identified a mutation in the ligand binding domain (LBD) of the RAR portion of PML/RAR. Results show that these retinoid-resistant NB4 cells require persistent PML/RAR expression for leukemic cell growth. Taken together, these findings can account for why these cells do not respond to ATRA and how reduction of PML/RAR abrogates the antiapoptotic effect it confers to these leukemic cells. © 1998 by The American Society of Hematology.
The evidence of mycorrhizal fungi and dark septate endophytes in roots of Chlorophytum borivillianum (2008)

Mycorrhizal and dark septate endophyte in Chlorophytum borivillianum cultivated in different parts of south-west India was found.Dark septate fungi with melanized hyphae and microsclerotia were documented.During culturing of the microsclerotia-containing roots, fungi with `Rhizoctonia-like' characters developed.The fungi showed reluctance in sporulation but produced microsclerotia in the medium.Specific taxa and their ecological role can be attributed only after detailed studies as different strains of single taxa may vary greatly in their physiology.
Welfare Administration and the Rights of Welfare Recipients (1977)

Public assistance programs and the rights of welfare recipients are controversial subjects confronting the legislature and the judiciary. Justice Tobriner has actively ensured that assistance programs are properly administered and that welfare recipients are accorded proper respect and constitutional protection. In this Article, the author surveys the development of the law of public assistance and Justice Tobriner's contributions in this field.
Fast analytical scatter estimation using graphics processing units (2015)

The analytical scatter estimator, implemented using graphics processing units, provides rapid and accurate estimates of single scatter and with further acceleration and a method to account for multiple scatter may be useful for practical scatter correction schemes.
Heart failure and the aging population: an increasing burden in the 21st century? (2002)

Unless rapid and major changes occur in the incidence of heart failure, the burden of this disorder will continue to increase in both primary and secondary care over the next two decades. The greatest increase is likely to occur in men. Future health service planning must take this into account.
Breast cancer-specific mutations in CK1ε inhibit Wnt/β-catenin and activate the Wnt/Rac1/JNK and NFAT pathways to decrease cell adhesion and promote cell migration (2010)

In summary, these data suggest that the mutations of CK1epsilon found in breast cancer can suppress Wnt/beta-catenin as well as promote the Wnt/Rac-1/JNK and Wnt/NFAT pathways, thus contributing to breast cancer development via effects on cell adhesion and migration. In terms of molecular mechanism, our data indicate that the breast cancer point mutations in the N-terminal lobe of CK1epsilon, which are correlated with decreased phosphorylation activities of mutated forms of CK1epsilon both in vitro and in vivo, interfere with positive autophosphorylation at Thr 44.