gebhart/patrepeval huggingface dataset:
The three genes lipB, lipC, and lipD involved in the extracellular secretion of the Serratia marcescens lipase which lacks an N-terminal signal peptide (1995)

The extracellular lipase of Serratia marcescens Sr41, lacking a typical N-terminal signal sequence, is secreted via a signal peptide-independent pathway. The 20-kb SacI DNA fragment which allowed the extracellular lipase secretion was cloned from S. marcescens by selection of a phenotype conferring the extracellular lipase activity on the Escherichia coli cells. The subcloned 6.5-kb EcoRV fragment was revealed to contain three open reading frames which are composed of 588, 443, and 437 amino acid residues constituting an operon (lipBCD). Comparisons of the deduced amino acid sequences of the lipB, lipC, and lipD genes with those of the Erwinia chrysanthemi prtDEC, prtEEC, and prtFEC genes encoding the secretion apparatus of the E. chrysanthemi protease showed 55, 46, and 42% identity, respectively. The products of the lipB and lipC genes were 54 and 45% identical to the S. marcescens hasD and hasE gene products, respectively, which were secretory components for the S. marcescens heme-binding protein and metalloprotease. In the E. coli DH5 cells, all three lipBCD genes were essential for the extracellular secretion of both S. marcescens lipase and metalloprotease proteins, both of which lack an N-terminal signal sequence and are secreted via a signal-independent pathway. Although the function of the lipD gene seemed to be analogous to those of the prtFEC and tolC genes encoding third secretory components of ABC transporters, the E. coli TolC protein, which was functional for the S. marcescens Has system, could not replace LipD in the LipB-LipC-LipD transporter reconstituted in E. coli. These results indicated that these three proteins are components of the device which allows extracellular secretion of the extracellular proteins of S. marcescens and that their style is similar to that of the PrtDEF(EC) system.
Tuning the permeability of permalloy films for on-chip inductor applications (2010)

In this work, we demonstrate that the permeability of 1 μm thick NiFe/Cr multilayer films can be tuned by varying the thickness of individual NiFe layers while keeping the total multilayer film thickness constant. Our results show that the permeability can be increased from 170 to 650 by varying the NiFe thicknesses from 100 to 50 nm. The thickness dependant permeability variation was attributed to the presence of stripe domains, as examined by hysteresis measurements and magnetic force microscope imaging. Films with thinner NiFe layers coupled with on-chip spiral inductors enable an inductance increase by a factor of 6.
Lithium Intercalation Compound Dramatically Influences the Electrochemical Properties of Exfoliated MoS<sub>2</sub> (2014)

MoS2 and other transition metal dichalcogenides (TMDs) have recently gained a renewed interest due to the interesting electronic, catalytic, and mechanical properties which they possess when down-sized to single or few layer sheets. Exfoliation of the bulk multilayer structure can be achieved by a preliminary chemical Li intercalation followed by the exfoliation due to the reaction of Li with water. Organolithium compounds are generally adopted for the Li intercalation with n-butyllithium (n-Bu-Li) being the most common. Here, the use of three different organolithium compounds are investigated and compared, i.e., methyllithium (Me-Li), n-butyllithium (n-Bu-Li) and tert-butyllithium (t-Bu-Li), used for the exfoliation of bulk MoS2 . Scanning transmission electron microscopy (STEM), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and cyclic voltammetry (CV) are adopted for a comprehensive characterization of all materials under investigation. In addition, catalytic properties towards the hydrogen evolution reaction (HER) and capacitive properties are also tested. Different organolithium compounds exhibit different extent of Li intercalation resulting in different degrees of exfoliation. The inherent electrochemical behavior of MoS2 consisting of significant anodic and cathodic peaks as well as its capacitive behavior and catalytic properties towards hydrogen evolution reaction are strongly connected to the exfoliation compound used. This research significantly contributes to the development of large-scale synthesis of electrocatalytic MoS2 -based materials.
Synthesis and Photoluminescent Properties of (La,Ca)<sub>3</sub>Si<sub>6</sub>N<sub>11</sub>:Ce<sup>3+</sup> Fine Powder Phosphors for Solid-State Lighting (2011)

We have developed a new Ce(3+)-activated nitride phosphor, (La,Ca)(3)Si(6)N(11):Ce(3+), using the gas-reduction-nitridation method. The synthesized (La,Ca)(3)Si(6)N(11):Ce(3+) possesses tunable yellow broadband emission with the dominant wavelength of 577-581 nm and the external quantum efficiency up to ∼42%, under an excitation of 450 nm. Precise steady-state and time-resolved photoluminescence analyses revealed that the only one type of Ce(3+) center is active under the blue-light excitation. By combining the synthesized (La,Ca)(3)Si(6)N(11):Ce(3+) phosphors with the 450-nm InGaN chip, a broad range of white light with the correlated color temperatures of ∼2600-3800 K can be created, demonstrating their promising applicability to the warm-white light-emitting diodes.
A simulation study of the mechanisms to enhance TCP protocol in wide area computer networks (1996)

Article Free Access Share on A simulation study of the mechanisms to enhance TCP protocol in wide area computer networks Authors: K. M. Khalil Bellcore BellcoreView Profile , Y. S Sun National Taiwan University National Taiwan UniversityView Profile , M. S. Obaidat City University of New York City University of New YorkView Profile Authors Info & Claims SAC '96: Proceedings of the 1996 ACM symposium on Applied ComputingFebruary 1996 Pages 77–81https://doi.org/10.1145/331119.331149Published:18 February 1996Publication History 0citation306DownloadsMetricsTotal Citations0Total Downloads306Last 12 Months6Last 6 weeks1 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Neuroprotective activity of a new class of steroidal inhibitors of the <i>N</i> -methyl- <scp>d</scp> -aspartate receptor (1997)

Release of the excitatory neurotransmitter glutamate and the excessive stimulation of N-methyl-D-aspartate (NMDA)-type glutamate receptors is thought to be responsible for much of the neuronal death that occurs following focal hypoxia-ischemia in the central nervous system. Our laboratory has identified endogenous sulfated steroids that potentiate or inhibit NMDA-induced currents. Here we report that 3alpha-ol-5beta-pregnan-20-one hemisuccinate (3alpha5betaHS), a synthetic homologue of naturally occurring pregnanolone sulfate, inhibits NMDA-induced currents and cell death in primary cultures of rat hippocampal neurons. 3alpha5betaHS exhibits sedative, anticonvulsant, and analgesic properties consistent with an action at NMDA-type glutamate receptors. Intravenous administration of 3alpha5betaHS to rats (at a nonsedating dose) following focal cerebral ischemia induced by middle cerebral artery occlusion significantly reduces cortical and subcortical infarct size. The in vitro and in vivo neuroprotective effects of 3alpha5betaHS demonstrate that this steroid represents a new class of potentially useful therapeutic agents for the treatment of stroke and certain neurodegenerative diseases that involve over activation of NMDA receptors.
Transport of anti-sperm monoclonal IgA and IgG into murine male and female genital tracts from blood. Effect of sex hormones (1996)

Ab levels in the genital tract may be important in fertility and in preventing sexually transmitted diseases. In this study, 125I-labeled polymer or monomer mAb IgA (C4pIgA or C4mIgA) and IgG2b (C4IgG) to murine lactate dehydrogenase C4 and a polymer mAb IgA (nplgA) not cross-reacting with mouse sperm were intravenously injected into BALB/c mice, and the relative distribution of these Abs was determined. Polymer IgA was transported much more efficiently into the genital tract, trachea, and duodenum of both sexes than C4IgG and C4 mIgA (p < 0.01). The transport of polymer IgA (C4pIgA and npIgA) into the male genital tract greatly increased following orchiectomy (p < 0.01); this change was not affected by testosterone, suggesting that the unknown regulatory factor(s) from the testis may suppress polymer IgA transport. However, the transport of polymer IgA into female genital tissues was significantly decreased by ovariectomy (p < 0.01); this decline can be rectified by beta-estradiol but not progesterone treatment, suggesting that estradiol may stimulate polymer IgA transport. Furthermore, the transport of C4IgG into tissues of the Fallopian tubes and the uterus was significantly decreased by treatment with progesterone (p < 0.01). Together, these findings indicate that serum polymer IgA can be transported selectively into the genital tracts of both sexes, that this transport is strongly under the control of gonads, and that transport of IgG into the Fallopian tubes and uterus is down-regulated by progesterone.
Current challenges of interactive digital television (2017)

This article presents a high level overview of the current challenges that Interactive Digital Television involves due to the changes in user's behaviour under the current TV watching experience. We present a short review of multiple works found in the literature which have addressed specific issues that arise on converting an only-watching setting onto an interactive experience. Moreover, we have envisioned a system architecture that evidence how second screen accompanying devices, such as mobile phones or tablets, can be used to facilitate the interactivity process from the technical and operative viewpoints. In particular, this architecture supports innovative interactive applications for automatic ad recognition utilizing a second screen device as a friendly user interface.
Synthesis, Characterization, Antimicrobial Activity, and Adhesive Property of Amino Benzyl Group Substituted Acrylic/Methacrylic Polymers and Their Copolymers (2013)

The monomers 2-aminobenzylacrylate (2-ABA) and 2-aminobenzyl methacrylate (2-ABMA) were synthesized. The homopolymers and various compositions of copolymers with ethoxylated bisphenol-A-dimethacrylate (EBPADMA) were synthesized using free radical polymerization. The polymers were characterized by FT-IR, 1H NMR, and 13C NMR spectral techniques. The thermal property of the polymers was determined by thermogravimetric analysis and differential scanning calorimeter. The prepared adhesives were tested on leather and metal surfaces using single-lap-joint shear test method. The antimicrobial activity of polymers and copolymers has been studied using Mueller Hinton Broth method.
Comparative Antibody-Mediated Phagocytosis of<i>Staphylococcus epidermidis</i>Cells Grown in a Biofilm or in the Planktonic State (2006)

Staphylococcus epidermidis is an important cause of nosocomial infections. Virulence is attributable to elaboration of biofilms on medical surfaces that protect the organisms from immune system clearance. Even though leukocytes can penetrate biofilms, they fail to phagocytose and kill bacteria. The properties that make biofilm bacteria resistant to the immune system are not well characterized. In order to better understand the mechanisms of resistance of bacteria in biofilms to the immune system, we evaluated antibody penetration throughout the biofilm and antibody-mediated phagocytic killing of planktonic versus biofilm cells of S. epidermidis by using a rabbit antibody to poly-N-acetylglucosamine (PNAG). These antibodies are opsonic and protect against infection with planktonic cells of PNAG-positive Staphylococcus aureus and S. epidermidis. Antibody to PNAG readily penetrated the biofilm and bound to the same areas in the biofilm as did wheat germ agglutinin, a lectin known to bind to components of staphylococcal biofilms. However, biofilm cells were more resistant to opsonic killing than their planktonic counterparts in spite of producing more PNAG per cell than planktonic cells. Biofilm extracts inhibited opsonic killing mediated by antibody to PNAG, suggesting that the PNAG antigen within the biofilm matrix prevents antibody binding close to the bacterial cell surface, which is needed for efficient opsonic killing. Increased resistance of biofilm cells to opsonic killing mediated by an otherwise protective antibody was due not to a biofilm-specific phenotype but rather to high levels of antigen within the biofilm that prevented bacterial opsonization by the antibody.
Glycoproteomics for Prostate Cancer Detection: Changes in Serum PSA Glycosylation Patterns (2008)

Currently, serum prostate-specific antigen (PSA) is used for the early detection of prostate cancer despite its low specificity in the range of 4-10 ng/mL. Because aberrant glycosylation is a fundamental characteristic of tumor genesis, the objective of this study was to investigate whether changes in PSA glycosylation may be used to improve the cancer specificity of PSA. We developed five lectin immunosorbant assays to analyze the glycosylation patterns of PSA in serum. Each assay sandwiches serum PSA between a PSA monoclonal antibody and a biotinylated lectin and then tags the biotin complex using a streptavidin SULFO TAG for electrochemiluminescence detection. Low limits of detection (0.04-1.35 ng/mL), good reproducibility (%CVs < 10%), and direct analysis of PSA glycosylation in sera suggest these assays may have a potential role in improving PSA's cancer specificity. Clinical performance was evaluated in 52 human subjects (26 cancer and 26 noncancer). ROC analysis showed that the total SNA assay (AUC = 0.71) appeared to perform better than percent free PSA (AUC = 0.54) in its diagnostic gray zone between 10 and 20% in a subset of 21 subjects. A separate study of 16 additional subjects showed similar findings.
Physically Unclonable Functions Using Foundry SRAM Cells (2018)

This paper describes a low voltage physically unclonable function (PUF) implemented with SRAM circuits. The approach allows the use of foundry cells, which are used in this paper, and requires very minor modifications to standard SRAM arrays. The PUF functionality is designed into large 1M-bit SRAM arrays fabricated on a 55-nm process using the foundry supplied SRAM cell layouts. The low variability foundry process produces good PUF results, demonstrating that the approach should also be good on conventional processes, since greater mismatch should positively impact PUF performance as measured by code word stability. The impact of process corners is also experimentally determined. Unstable bits, which we attribute to random telegraph noise is shown to be at manageable levels. We describe the circuit operation, statistical behavior, and suggest helper data functions that allow operation without error correction. This is important since error correction necessarily allows some leakage of the underlying secret codes.
Current and Emerging Azole Antifungal Agents (1999)

Major developments in research into the azole class of antifungal agents during the 1990s have provided expanded options for the treatment of many opportunistic and endemic fungal infections. Fluconazole and itraconazole have proved to be safer than both amphotericin B and ketoconazole. Despite these advances, serious fungal infections remain difficult to treat, and resistance to the available drugs is emerging. This review describes present and future uses of the currently available azole antifungal agents in the treatment of systemic and superficial fungal infections and provides a brief overview of the current status of in vitro susceptibility testing and the growing problem of clinical resistance to the azoles. Use of the currently available azoles in combination with other antifungal agents with different mechanisms of action is likely to provide enhanced efficacy. Detailed information on some of the second-generation triazoles being developed to provide extended coverage of opportunistic, endemic, and emerging fungal pathogens, as well as those in which resistance to older agents is becoming problematic, is provided.
Polymorphisms of co-inhibitory molecules (CTLA-4/PD-1/PD-L1) and the risk of non-small cell lung cancer in a Chinese population. (2015)

Lung cancer is a leading cause of cancer-related death in China, with non-small cell lung cancer (NSCLC) comprises the most common form. Co-inhibitory molecules, such as cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), programmed death 1 (PD-1) and its ligand PD-L1, play a key roles in the physiopathological process of tumorigenesis. To investigate whether genetic variations of co-inhibitory molecules are associated with the risk of NSCLC, we analyzed polymorphisms of CTLA-4 (-318, +49), PD-1 (PD-1.1, PD-1.3, PD-1.5, PD-1.9) and PD-L1 (+8293) in a cohort of 528 NSCLC subjects and 600 healthy controls. By restriction fragment length polymorphism (RFLP) method, we found that the distributions of the CTLA-4 and PD-1 gene polymorphisms were similar between NSCLC patients and healthy controls. However, for the PD-L1 8923 A/C polymorphism, frequencies of the AC genotype and C-allele were significantly higher in NSCLC patients than in healthy controls (odds ratio [OR] =1.55; 95% confidence interval [CI] 1.13-2.13; P=0.006; OR=1.52; 95% CI 1.14-2.04; P=0.004, respectively). Stratification analysis revealed that prevalence of the 8923C allele was significantly increased in NSCLC patients who smoke compared to those non-smoking patients (OR=1.51; 95% CI 1.00-2.28; P<0.05). Moreover, NSCLC patients carrying the C-allele had higher risk of regional lymph node metastasis than those carrying the A-allele (OR=5.65; 95% CI 2.45~13.03; P<0.001). These data suggest that PD-L1+8293A>C polymorphism may play a role in the development and progression of NSCLC.
Clustered ARAIM (2016)

Modernization of existing and deployment of new GNSS constellations requires re-evaluation of current integrity monitoring schemes. The baseline ARAIM algorithm, which is being developed to become the next-generation standard method to ensure enough confidence in the computed position, requires significantly higher computational effort than the current integrity monitoring methods. This paper presents a method which greatly reduces this complexity while preserving the original performance in terms of protection levels. That is achieved by creating sub-solutions that cover several fault-modes at the time, reducing the total number of sub-solutions to be assessed. Moreover, an additional method to streamline a different extensively used part of the ARAIM algorithm is introduced.
Expression of the symbiotic plasmid from <i>Rhizobium leguminosarum</i> biovar <i>trifolii</i> in <i>Sphingobacterium multivorum</i> (1994)

An inoculant strain of Rhizobium leguminosarum biovar trifolii containing a Tn5 marked symbiotic plasmid transferred this plasmid by conjugation to Sphingobacterium multivorum, an organism that can be found in soil. The transconjugant bacteria nodulated the roots of white clover (Trifolium repens) seedlings but did not fix atmospheric nitrogen. Microscopic examination revealed abnormal nodule structures. Bacteria isolated from the nodules were shown to be closely related to the recipient S. multivorum and Southern blots of genomic digests probed with nodA DNA confirmed that the transconjugants contained symbiotic genes. This is the first report of the spontaneous transfer, by conjugation, of a symbiotic plasmid from R. leguminosarum biovar trifolii to S. multivorum.
Object-oriented software: theory, practice, and implementation (2002)

There are several themes underlying the object-oriented paradigm. Although these themes are not unique to object-oriented technology, they are particularly well supported in object oriented systems. The paper discusses some of these themes, including: abstraction, encapsulation, combining data and behavior, data and behavior sharing, emphasis on object structure rather than procedure structure, and software synergy.
Stylized facts of daily return series and the hidden Markov model (1998)

In two recent papers, Granger and Ding (1995a,b) considered long return series that are first differences of logarithmed price series or price indices. They established a set of temporal and distributional properties for such series and suggested that the returns are well characterized by the double exponential distribution. The present paper shows that a mixture of normal variables with zero mean can generate series with most of the properties Granger and Ding singled out. In that case, the temporal higher-order dependence observed in return series may be described by a hidden Markov model. Such a model is estimated for ten subseries of the well-known S&P 500 return series of about 17,000 daily observations. It reproduces the stylized facts of Granger and Ding quite well, but the parameter estimates of the model sometimes vary considerably from one subseries to the next. The implications of these results are discussed. © 1998 John Wiley & Sons, Ltd.
Boosting SVM Classifiers with Logistic Regression (2003)

The support vector machine classifier is a linear maximum margin classifier. It performs very well in many classification applications. Although, it could be extended to nonlinear cases by exploiting the idea of kernel, it might still su#er from the heterogeneity in the training examples. Since there are very few theories in the literature to guide us on how to choose kernel functions, the selection of kernel is usually based on a try-and-error manner.
<i>Bacillus cereus</i>, the causative agent of an emetic type of food‐borne illness (2004)

Bacillus cereus is the causative agent of two distinct forms of gastroenteritic disease connected to food-poisoning. It produces one emesis-causing toxin and three enterotoxins that elicit diarrhea. Due to changing lifestyles and eating habits, B. cereus is responsible for an increasing number of food-borne diseases in the industrial world. In the past, most studies concentrated on the diarrhoeal type of food-borne disease, while less attention has been given to the emetic type of the disease. The toxins involved in the diarrhoeal syndrome are well-known and detection methods are commercially available, whereas diagnostic methods for the emetic type of disease have been limited. Only recently, progress has been made in developing identification methods for emetic B. cereus and its corresponding toxin. We will summarize the data available for the emetic type of the disease and discuss some new insights in emetic strain characteristics, diagnosis, and toxin synthesis.
1 /spl mu/m MOSFET VLSI technology. III. Logic circuit design methodology and applications (1979)

For pt. II see ibid., vol.SC14, no.2, p.247 (1979). Logic circuits were designed and fabricated in a 1 /spl mu/m silicon-gate MOSFET technology. First, conventional random logic chip images using the largely one-dimensional `Weinberger' layout are examined. The image is able to provide chips with an average circuit delay of 3 ns at the 8000 circuit level of integration. Second, two forms of PLA and PLA-based macros are discussed. A dynamic PLA, used in a microprocessor cross section and including 105 product terms, which achieves a 56 ns cycle time is described. A static PLA, designed for 21-ns delay and achieving measured delays from 13 to 21 ns, is also described. Extensions, particularly into low-temperature operation, are discussed.