gebhart/patrepeval huggingface dataset:
Human fibrinogen specifically binds hyaluronic acid. (1986)

Fibrin and hyaluronic acid (HA) are macromolecules whose concentrations are elevated at the same time in the extracellular space of damaged tissues.We have investigated whether HA can bind to fibrinogen using solid phase and soluble assays.Purified human fibrinogen specifically bound to HA-Sepharose to a greater extent (>b-fold) than did ul-acid glycoprotein, DNaseI, ovalbumin, haptoglobin, or lysozyme.Fibrinogen did not bind to ethanolamine-Sepharose, a control chromatographic support.Treatment of HA-Sepharose containing bound '"I-fibrinogen with ovine testicular hyaluronidase released 44% of the lz61 radioactivity, indicating that fibrinogen was specificaliy bound to HA.Moreover, 12'I-fibrinogen bound to HA-Sepharose could be displaced by free HA but not by either of the monosaccharide components of this polymer, glucuronic acid, or N-acetylglucosamine. Chondroitin sulfate and polygalacturonic acid competed only weakly for bound '2aI-fibrinogen.Bound "4-fibrinogen was also not released by high concentrations of NaCl (up to 4 M), indicating that the interaction is not simply ionic.The apparent affinity of fibrinogen for HA covaried with the molecular weight of the HA.Small HA oligosaccharides (M, = 3900) were only 50% as effective as larger HA (Mr = 8 X 10") in eluting bound '261-fibrinogen from HA-Sepharose.The optimal oligosaccharide size for displacement of bound "'I-fibrinogen was 2200 monosaccharides.Additionally, the amount of "1-fibrinogen bound to HA-Sepharose was directly related to the size of the HA-amine linked to the affinity support.The affinity constant for fibrinogen binding to 12'I-HA (-150 monosaccharides) is estimated to be at least 2 X lo7These results demonstrate for the first time a specific, reversible binding between HA and fibrinogen.Proteolytic activation of the soluble plasma protein fibrinogen transforms it into fibrin, which forms an insoluble polymeric gel and aids in the prevention of blood loss (1).Although fibrin(ogen) has received little attention as a structural or an organizational component of the ECM' in repairing
Exploration of suitable dry etch technologies for directed self-assembly (2012)

Directed self-assembly (DSA) has shown the potential to replace traditional resist patterns and provide a lower cost alternative for sub-20-nm patterns. One of the possible roadblocks for DSA implementation is the ability to etch the polymers to produce quality masks for subsequent etch processes. We have studied the effects of RF frequency and etch chemistry for dry developing DSA patterns. The results of the study showed a capacitively-coupled plasma (CCP) reactor with very high frequency (VHF) had superior pattern development after the block co-polymer (BCP) etch. The VHF CCP demonstrated minimal BCP height loss and line edge roughness (LER)/line width roughness (LWR). The advantage of CCP over ICP is the low dissociation so the etch rate of BCP is maintained low enough for process control. Additionally, the advantage of VHF is the low electron energy with a tight ion energy distribution that enables removal of the polymethyl methacrylate (PMMA) with good selectivity to polystyrene (PS) and minimal LER/LWR. Etch chemistries were evaluated on the VHF CCP to determine ability to treat the BCPs to increase etch resistance and feature resolution. The right combination of RF source frequencies and etch chemistry can help overcome the challenges of using DSA patterns to create good etch results.
Performance analysis of a multicast switch (1991)

The performance of a multicast switch, a space division switch in which a packet at an input port is routed to a subset of the output ports, is discussed. The delay under the assumption of input port buffering is determined. The system is modeled as an independent set of M/G/1 queues. A key assumption in the analysis is that output port contention is settled by random selection among the contending input ports. In order to justify this as well as the other assumptions, a Monte-Carlo simulation of the system is devised. Numerical results from the analysis and simulation of the system are presented.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Neutrophils lacking phosphoinositide 3-kinase γ show loss of directionality during <i>N</i> -formyl-Met-Leu-Phe-induced chemotaxis (2002)

Confocal imaging and time-lapsed videomicroscopy were used to study the directionality, motility, rate of cell movement, and morphologies of phosphoinositide 3-kinase gamma (PI3K)gamma(-/-) neutrophils undergoing chemotaxis in Zigmond chambers containing N-formyl-Met-Leu-Phe gradients. Most of the PI3Kgamma(-/-) neutrophils failed to translocate up the chemotactic gradient. A partial reduction in cell motility and abnormal morphologies were also observed. In the wild-type neutrophils, the pleckstrin homology domain-containing protein kinase B (AKT) and F-actin colocalize to the leading edge of polarized neutrophils oriented toward the gradient, which was not observed in PI3Kgamma(-/-) neutrophils. In PI3Kgamma(-/-) neutrophils, AKT staining consistently failed to perfectly overlap with the F-actin. This failure was observed as an F-actin-filled region of 2.3 +/- 0.5 microm between AKT and the cell membrane. These data suggest that PI3Kgamma regulates neutrophil chemotaxis primarily by controlling the direction of cell migration and the intracellular colocalization of AKT and F-actin to the leading edge.
A Leakage Current Replica Keeper for Dynamic Circuits (2007)

We present a leakage current replica (LCR) keeper for dynamic domino gates that uses an analog current mirror to replicate the leakage current of a dynamic gate pull-down stack and thus tracks process, voltage, and temperature. The proposed keeper has an overhead of one field-effect transistor per gate plus a portion of a shared current mirror. Techniques for properly sizing LCR keepers are presented. Using these sizings, LCR keepers allow design of and-or circuits with 30% more legs than conventional keepers at the same noise margin in a 90-nm, 1.2-V CMOS logic process. Furthermore, 16-24-leg dynamic AO circuits are 25%-40% faster when using the replica keeper. We demonstrated the circuit operation on a 1024 words times 72 bits, 3W/4R embedded SRAM macro using a four-stage LCR-keeper domino structure for a read-out circuit
Shaped Electroluminescent GaAs Diodes (1964)

Electroluminescent GaAs diodes have been shaped as truncated cones with the p-n junction at the apex to direct a large fraction of the radiation toward the base of the cone within the critical angle for transmission. Devices having an efficiency at 77°K of 8% have been fabricated. Efficiency is defined here as the radiant power out the base per unit electrical power input. It is calculated that improved devices of this type will have efficiencies of about 18%.
Sex Differences in Cholinergic Analgesia I  (1999)

These data demonstrate a large sex difference in antinociception to intrathecal neostigmine that is primarily the result of a nicotinic component in females. Phentolamine reversal suggests that part of this nicotinic component may rely on spinal norepinephrine release. A better understanding of this sex difference could lead to development of novel pain therapy for women.
Photochemistry of cycloalkenes. 9. Photodimerization of cyclohexene (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemistry of cycloalkenes. 9. Photodimerization of cyclohexenePaul J. Kropp, John J. Snyder, Peter C. Rawlings, and Harold G. Fravel Jr.Cite this: J. Org. Chem. 1980, 45, 22, 4471–4474Publication Date (Print):October 1, 1980Publication History Published online1 May 2002Published inissue 1 October 1980https://pubs.acs.org/doi/10.1021/jo01310a039https://doi.org/10.1021/jo01310a039research-articleACS PublicationsRequest reuse permissionsArticle Views360Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Stimulation of phospholipase C by guanine‐nucleotide‐binding protein βγ subunits (1992)

We have previously shown that soluble fractions obtained from human HL-60 granulocytes contain a phospholipase C which is markedly stimulated by the stable GTP analogue guanosine 5'-[3-O-thio]triphosphate (Camps, M., Hou, C., Jakobs, K. H. and Gierschik, P. (1990) Biochem. J. 271, 743-748]. To investigate whether this stimulation was due to a soluble alpha subunit of a heterotrimeric guanine-nucleotide-binding protein or a soluble low-molecular-mass GTP-binding protein, we have examined the effect of purified guanine-nucleotide-binding protein beta gamma dimers on the phospholipase-C-mediated formation of inositol phosphates by HL-60 cytosol. We found that beta gamma subunits, purified from bovine retinal transducin (beta gamma t), markedly stimulated the hydrolysis of phosphatidylinositol 4,5-bisphosphate by this phospholipase C preparation. The stimulation of phospholipase C by beta gamma t was not secondary to a phospholipase-A2-mediated generation of arachidonic acid, was prevented by the GDP-liganded transducin alpha subunit and was additive to activation of phospholipase C by guanosine 5'-[3-O-thio]triphosphate. Beta gamma t also stimulated soluble phospholipase C from human and bovine peripheral neutrophils, as well as membrane-bound, detergent-solubilized phospholipase C from HL-60 cells. Stimulation of soluble HL-60 phospholipase C was not restricted to beta gamma t, but was also observed with highly purified beta gamma subunits from bovine brain. Fractionation of HL-60 cytosol by anion-exchange chromatography revealed the existence of at least two distinct forms of phospholipase C in HL-60 granulocytes. Only one of these forms was sensitive to stimulation by beta gamma t, demonstrating that stimulation of phospholipase C by beta gamma subunits is isozyme specific. Taken together, our results suggest that guanine-nucleotide-binding protein beta gamma subunits may play an important and active role in mediating the stimulation of phospholipase C by heterotrimeric guanine-nucleotide-binding proteins.
Tau in Alzheimer neurofibrillary tangles. <i>N</i>- and <i>C</i>-terminal regions are differentially associated with paired helical filaments and the location of a putative abnormal phosphorylation site (1991)

To investigate the extent to which whole tau proteins, structurally abnormal tau and fragments of tau are incorporated into neurofibrillary tangles in Alzheimer's disease, an immunocytochemical mapping study using a panel of antibodies to several synthetic human tau peptides has been performed. Neurofibrillary tangles were immunolabelled in situ, and paired helical filaments (PHF), the principal structural component of tangles, were immunolabelled after isolation and Pronase treatment. N-Terminal and C-terminal domains of tau were found to be present in tangles in situ. SDS-treated PHF were found to contain most of the C-terminal half of tau and were also labelled by antibodies to ubiquitin. Only some of these PHF were labelled by antisera to tau sequences towards the N-terminus, and this enabled the identification of a region of tau in which proteolytic cleavage may occur. The ultrastructural appearance of the immunolabelling suggested that both the N- and C-terminal domains of tau extend outwards from the axis of PHF. After Pronase treatment. PHF were strongly labelled only by an antiserum to PHF and by the antiserum to the most C-terminal tau synthetic peptide. The latter antiserum also strongly labelled extracellular tangles in situ, whereas these extracellular tangles were poorly labelled by the antisera to the other synthetic peptides. One anti-(tau peptide) serum labelled a population of neurofibrillary tangles in situ only after alkaline phosphatase pretreatment of tissue sections. Our results show that, although peptides along the length of the tau molecule are associated with neurofibrillary tangles in situ, only the C-terminal one-third of the molecule is tightly associated with PHF, since this region of tau is resistant to SDS treatment of PHF. We also report the existence in PHF in situ of a masked tau epitope which is partially unmasked by dephosphorylation. These results are indicative of post-translational changes in tangle-associated tau in degenerating neurons in Alzheimer's disease.
Moisture‐induced effects on the tensile mechanical properties and glass‐transition temperature of poly(vinyl alcohol) films (2011)

Abstract In this study, the effect of sorbed water on the tensile mechanical properties of noncrosslinked, thermally treated poly(vinyl alcohol) (PVA) films was studied. The Young's modulus, elongation at break, and tensile strength of the PVA films equilibrated at different relative humidities (0–86%) are reported, together with the depression of the glass transition of the polymer at each equilibrating humidity, as determined by temperature‐modulated differential scanning calorimetry. The results indicate that drastic changes in the tensile properties were correlated with the transition of the hydrated polymer from the glassy to the rubbery state. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Digital holographic data storage prototype (2002)

With the invention of new multiplexing and system techniques combined with a new type of storage material, Bell Labs has made significant strides towards establishing the commercial feasibility of holographic data storage. A density of 48.6 bits//spl mu/m/sup 2/ has been achieved in a photopolymer medium. Materials in hand will result in 5 1/4 inch disks with 125 GB of user capacity, >40 MB/s read rates, random access, and low cost removable media.
Coronary Artery Spasm Demonstrated by Coronary Angiography in a Patient with Acute Myocarditis Resembling Acute Myocardial Infarction; A Case Report. (1991)

A 59-year-old male with acute myocarditis presenting symptoms resembling acute myocardial infarction underwent urgent coronary angiography due to difficulties in determining a diagnosis. Coronary artery spasm was demonstrated in the proximal right and left anterior descending coronary arteries. The spasm varied from time to time and lesion to lesion. But no fixed occluded lesion was observed. Predischarge coronary angiography showed no abnormal findings. Histological findings of the biopsy specimen from the left ventricle were consistent with myocarditis. The findings of this case indicate that a coronary artery spasm may aggravate the myocardial damage occurring in acute myocarditis. Urgent coronary angiography and endomyocardial biopsy are important to make the diagnosis of myocarditis in patients presenting symptoms resembling acute myocardial infarction.
Measurement of nonlinear absorption and refraction in titanium dioxide single crystal by using a phase distortion method (1995)

A phase distortion method is applied to examine the optical nonlinearity in titanium dioxide single crystal. The two-photon absorption coefficient obtained agrees with that reported previously, while the nonlinear index of refraction is larger than that predicted theoretically by a factor of 10. One of the reasons for the discrepancy is given as the applicable limit of the simple theory in which a two-parabolic band model has been assumed in the analysis.
Evaluation of methods for shaded surface display of CT-volumes (2003)

Twelve methods are evaluated to analyze an ideal sphere and a real human skull. They are different combinations of principles for detection of the surface to be displayed (gray-value threshold, gradient threshold, and zero-crossing of the second derivative), localizing this surface in space (including both grid-point accuracy and subvoxel accuracy), and finally estimating the direction of the surface normal (from the gradient in the 2-D depth image and from the gradient in the 3-D volume). The best quality is obtained by zero-crossing detection, subvoxel localization, and 3-D gradient orientation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Disinfection of Male Luer Style Connectors for Prevention of Catheter Related Bloodstream Infections Using an Isopropyl Alcohol Dispensing Cap (2010)

Bacterial colonization of needleless injection sites (NISs) frequently results in catheter related bloodstream infections (CRBSIs). Hospitals have instituted protocols aimed at disinfecting NIS prior to access. Furthermore, several manufactures have developed devices that facilitate disinfection of NIS. Despite these steps, the incidence of CRBSI is still alarmingly high. Currently, there is no protocol or device intended to disinfect male luer connectors such as those found on IV tubing that are commonly coupled and decoupled from the NISs. Since these IV tubing connectors directly contact the NIS (which have been repeatedly shown to have varying levels of bacterial colonization), it is highly likely that they, too, will have varying levels of contamination. In order for disinfection of the NIS to be effective, the IV tubing connector must also be disinfected. Our design goal was to develop a device that could be used to disinfect a male luer style connector without allowing antiseptic into the inner lumen of the male luer. We designed a three component system that utilizes a silicone sealing cone to seal the male luer, a reservoir foam that holds 70% isopropyl alcohol (IPA), and a reaction force foam that increases the seal pressure of the sealing cone while the reservoir foam is compressed delivering the IPA to the outside surface of the male luer post. Sealing cone geometry was optimized using a custom built seal pressure test apparatus. Reservoir and reaction force foam functional parameters were assessed using an Instron test apparatus. A two phase compression stroke was designed into the device to allow for sealing and dispensing of IPA. An IPA transfer test was used to assess the transfer of disinfectant from the reservoir foam to a liquid filled male luer connector (modeling an IV tubing connector). No disinfectant was found to be transferred from the device to the inner lumen of the IV tubing connector model (n=30). To test the efficacy of the device on reducing bacterial count on the male luer, a disinfection study was performed using the optimized device. Male luers were immersed in bacterial suspensions of S. aureus, S. epidermis, P. aerginosa, and E. coli. A 4 log reduction compared with a positive control was found in each sample treated with our disinfection cap (n=120). In conclusion, we developed a device that effectively delivers an antiseptic to a male luer style connector without leaking any antiseptic to the inner lumen of the luer post
Beta-sheet assembly of Tau and neurodegeneration in Drosophila melanogaster (2018)

The assembly of Tau into abundant β-sheet-rich filaments characterizes human tauopathies. A pathological pathway leading from monomeric to filamentous Tau is believed to be at the heart of these diseases. However, in Drosophila models of Tauopathy, neurodegeneration has been observed in the absence of abundant Tau filaments. Here we investigated the role of Tau assembly into β-sheets by expressing wild-type and Δ306-311 human Tau-383 in the retina and brain of Drosophila. We analyzed both lines for eye abnormalities, brain vacuolization, Tau phosphorylation and assembly, as well as climbing activity and survival. Flies expressing wild-type Tau-383 showed MC-1 staining, Tau hyperphosphorylation, and neurodegeneration. By contrast, flies expressing Δ306-311 Tau-383 had less MC-1 staining, reduced Tau hyperphosphorylation, and no detectable neurodegeneration. Their climbing ability and lifespan were similar to those of nontransgenic flies. Fluorescence spectroscopy after addition of Thioflavin T, a dye that interacts with β-sheets, showed no signal when Δ306-311 Tau-383 was incubated with heparin. These findings demonstrate that the assembly of Tau into β-sheets is necessary for neurodegeneration.
Piece by piece a picture (2013)

Making mosaics can give children an idea of how small parts can be used together to form a new piece, develops physical skills and allows them to be creative and imaginative, using materials in unusual ways.
FRET Quenching of Photosensitizer Singlet Oxygen Generation (2009)

The development of activatable photodynamic therapy (PDT) has demonstrated a utility for effective photosensitizer quenchers. However, little is known quantitatively about Forster resonance energy transfer (FRET) quenching of photosensitizers, even though these quenchers are versatile and readily available. To characterize FRET deactivation of singlet oxygen generation, we attached various quenchers to the photosensitizer pyropheophorbide-alpha (Pyro) using a lysine linker. The linker did not induce major changes in the properties of the photosensitizer. Absorbance and emission wavelength maxima of the quenched constructs remained constant, suggesting that quenching by ground-state complex formation was minimal. All quenchers sharing moderate spectral overlap with the fluorescence emission of Pyro (J > or = 5.1 x 10(13) M(-1) cm(-1) nm4) quenched over 90% of the singlet oxygen, and quenchers with weaker spectral overlap displayed minimal quenching. A self-quenched double Pyro construct exhibited intermediate quenching. Consistent with a FRET deactivation mechanism, extension of the linker to a 10 residue polyproline peptide resulted in only the quenchers with spectral overlap almost 2 orders of magnitude higher (J > or = 3.7 x 10(15) M(-1) cm(-1) nm4) maintaining high quenching efficiency. Overall, there was good correlation (0.98) between fluorescence quenching and singlet oxygen quenching, implying that fluorescence intensity can be a convenient indicator for the singlet oxygen production status of activatable photosensitizers. Uniform singlet oxygen luminescence lifetimes of the compounds, along with minimal triplet state transient absorption were consistent with quenchers primarily deactivating the photosensitizer excited singlet state. In vitro, cells treated with well-quenched constructs demonstrated greatly reduced PDT induced toxicity, indicating that FRET-based quenchers can provide a level of quenching useful for future biological applications. The presented findings show that FRET-based quenchers can potently decrease singlet oxygen production and therefore be used to facilitate the rational design of activatable photosensitizers.