gebhart/patrepeval huggingface dataset:
Robust and real-time multi-cue map verification for the road ahead (2014)

Digital maps are a key information for road course estimation in large distances, especially for automated driving with higher speeds or during nighttime. However, digital maps may contain errors caused by road changes or inaccurate recordings. As these errors may lead to severe application level failures, early and reliable detection of these errors, generally termed map verification, is a must. This work presents an online approach to verification of digital maps for automotive use based on neural multi-sensor fusion. Unlike previous works, the system is able to verify the digital map integrity ahead of the vehicle, before the error position is reached. Thus, appropriate countermeasures to mitigate possible effects can be initiated upfront. On the basis of a large database of map geometry and sensor information, a neural network is trained to classify the digital map integrity by optimally fusing sensor information at different abstraction levels. Using this technique, our system is able to verify the map integrity 100 meters ahead of the vehicle with an error rate of less than 3.4 %, while being real-time capable at the same time. Experiments validate the performance and robustness of this approach.
Characteristics of tertiary lymphoid structures in primary cancers (2013)

Tumors are sustained by complex networks of interactions between malignant cells, stromal cells and tumor-infiltrating immune cells. These networks differ from patient to patient in terms of nature, composition and organization as well as with regard to the precise localization of tumor-infiltrating cells. Of note, the heterogeneity of the immunological component of the tumor microenvironment, as opposed to its mere abundance, has been shown to influence disease outcome. However, a key question remains: where does the activation of tumor-specific T cells take place? The recently described, tumor-associated lymph node-like entities termed "tertiary lymphoid structures" exhibit a structural organization that is reminiscent of secondary lymphoid organs, and thus may imprint the local immune contexture. Here, we discuss how cancer-associated tertiary lymphoid structures impact on the tumor micro-architecture, immune microenvironment, and ultimately, patient survival.
Packaging designs for ultraviolet light emitting diodes (2012)

Aluminum Gallium Nitride (AlGaN) / Gallium Nitride (GaN) based deep ultraviolet (DUV) light emitting didoes (LEDs) with emission wavelengths between 200-280 nm enable key emerging technologies such as water/air purification and sterilization, covert communications and portable bio-agent detection/identification systems for homeland security, and surface and medical device sterilization. These devices produce a large amount of undesired heat due to low quantum efficiencies in converting electrical input to optical output. These low efficiencies are attributed to difficulties in the growth&doping of AlₓGa₁₋ₓN materials and UV absorbing substrates leading to excessive joule heating, which leads to device degradation and a spectral shift in the emission wavelength. With this regard, effective thermal management in these devices depends on the removal of this heat and reduction of the junction temperature. This is achieved by decreasing the package thermal resistance from junction-to-air with cost-effective solutions. The use of heat sinks, thermal interface materials, and high conductivity heat spreaders is instrumental in the reduction of the overall junction-to-air thermal resistance.\nThis thesis work focuses on thermal modeling of flip-chip packaged deep UV LEDs to gain a better understanding of the heat propagation through these devices as well as the package parameters that have the biggest contributions to reducing the overall thermal resistance. A parametric study focusing on components of a lead frame package is presented to ascertain the thermal impacts of various package layers including contact metallizations, thermal spreading sub-mounts, and thermal interface materials. In addition the use of alternative thermal interface materials such as phase change materials and liquid metals is investigated experimentally.
Hybridization of bacterial luciferase with a variant produced by chemical modification (1971)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHybridization of bacterial luciferase with a variant produced by chemical modificationE. A. Meighen, M. Ziegler Nicoli, and J. W. HastingsCite this: Biochemistry 1971, 10, 22, 4062–4068Publication Date (Print):October 1, 1971Publication History Published online1 May 2002Published inissue 1 October 1971https://pubs.acs.org/doi/10.1021/bi00798a008https://doi.org/10.1021/bi00798a008research-articleACS PublicationsRequest reuse permissionsArticle Views71Altmetric-Citations38LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
CRISPR/dCas9‐Mediated Multiplex Gene Repression in <i>Streptomyces</i> (2018)

Streptomycetes are Gram-positive bacteria with the capacity to produce copious bioactive secondary metabolites, which are the main source of medically and industrially relevant drugs. However, genetic manipulation of Streptomyces strains is much more difficult than other model microorganisms like Escherichia coli and Saccharomyces cerevisiae. Recently, CRISPR/Cas9 or dCas9-mediated genetic manipulation tools have been developed and facilitated Streptomyces genome editing. However, till now, CRISPR/dCas9-based interference system (CRISPRi) is only designed to repress single gene expression. Herein, the authors developed a novel CRISPRi system for multiplex gene repression in the model strain Streptomyces coelicolor. In this system, the integrative plasmid pSET152 is used as the backbone for the expression of the dCas9/sgRNA complex and both dCas9 and sgRNAs are designed to be under the control of constitutive promoters. Using the integrative CRISPRi system, the authors achieved efficient repression of multiple genes simultaneously; the mRNA levels of four targets are reduced to 2-32% of the control. Furthermore, it is successfully employed for functional gene screening, and an orphan response regulator (RR) (encoded by SCO2013) containing an RNA-binding ANTAR domain is identified being involved in bacterial growth. Collectively, this integrative CRISPRi system is very effective for multiplex gene repression in S. coelicolor, which could be extended to other Streptomyces strains for functional gene screening as well as for metabolic engineering.
Production of 3-hydroxypropionic acid from glycerol by acid tolerant<i>Escherichia coli</i> (2014)

The biological production of 3-hydroxypropionic acid (3-HP) has attracted significant attention because of its industrial importance. The low titer, yield and productivity, all of which are related directly or indirectly to the toxicity of 3-HP, have limited the commercial production of 3-HP. The aim of this study was to identify and select a 3-HP tolerant Escherichia coli strain among nine strains reported to produce various organic acids efficiently at high titer. When transformed with heterologous glycerol dehydratase, reactivase and aldehyde dehydrogenase, all nine E. coli strains produced 3-HP from glycerol but the level of 3-HP production, protein expression and activities of the important enzymes differed significantly according to the strain. Two E. coli strains, W3110 and W, showed higher levels of growth than the others in the presence of 25 g/L 3-HP. In the glycerol fed-batch bioreactor experiments, the recombinant E. coli W produced a high level of 3-HP at 460 ± 10 mM (41.5 ± 1.1 g/L) in 48 h with a yield of 31 % and a productivity of 0.86 ± 0.05 g/L h. In contrast, the recombinant E. coli W3110 produced only 180 ± 8.5 mM 3-HP (15.3 ± 0.8 g/L) in 48 h with a yield and productivity of 26 % and 0.36 ± 0.02 g/L h, respectively. This shows that the tolerance to and the production of 3-HP differ significantly among the well-known, similar strains of E. coli. The titer and productivity obtained with E. coli W were the highest reported thus far for the biological production of 3-HP from glycerol by E. coli.
Optical time-division channel drop and demultiplexing at 4:1 bit rate using a single Mach-Zehnder modulator in a fiber loop (2007)

A new optical time division demultiplexing technique is presented using a Mach-Zehdner modulator in a fiber loop mirror. Bit-error rate measurements show high- performance 4:1 demultiplexing of an 80 Gb/s optical signal to 20 Gb/s channels.
Plasma Deposited Silicon Nitride for Indium Phosphide Encapsulation (1989)

The composition and annealing characteristics of plasma deposited silicon nitride encapsulating films on ion implanted indium phosphide have been investigated. X‐ray photoelectron spectroscopy was employed to study the surface of substrates cleaned with organic solvents and or solutions prior to encapsulation. The composition of silicon nitride films deposited with 13.56 MHz RF excitation was determined through Auger electron spectroscopy and infrared spectroscopy both before and after annealing. The hydrogen concentration decreased from 21 to 9% during annealing. substrates implanted with silicon at 100 keV to a dose of and 150 keV to a dose of had activations of approximately 60% after annealing at 700°C. X‐ray photoelectron spectroscopic measurements were performed at five depths through the silicon nitride/indium phosphide interface of an annealed and an unannealed sample. No chemical interaction between the film and the substrate was observed before or after annealing. However, a change in the composition of the interfacial native oxides upon annealing is suggested from differences between the oxygen peaks for the unannealed and annealed samples.
The construction of <i>Bacillus thuringiensis</i> strains expressing novel entomocidal δ-endotoxin combinations (1990)

Using our recently reported method of electroporation to transform Bacillus thuringiensis [Bone & Ellar (1989) FEMS Microbiol. Lett. 58, 171-178], cloned B. thuringiensis entomocidal delta-endotoxin genes have been introduced into several native B. thuringiensis strains. In many cases the resulting transformants expressed both their native toxins and the cloned toxin, producing strains with broader toxicity spectra. The introduction of the var. tenebrionis toxin gene into B. thuringiensis var. israelensis resulted in a strain with activity against Pieris brassicae (cabbage white butterfly), an activity which neither parent strain possesses. We discuss further the possibility of synergism and also the problems associated with introducing cloned DNA by this method.
Ag<sub>44</sub>(SeR)<sub>30</sub>: A Hollow Cage Silver Cluster with Selenolate Protection (2013)

Selenolate protected, stable and atomically precise, hollow silver cluster was synthesized using solid state as well as solution state routes. The optical absorption spectrum shows multiple and sharp features similar to the thiolated Ag44 cluster, Ag44(SR)30 whose experimental structure was reported recently. High-resolution electrospray ionization mass spectrometry (HRESI MS) shows well-defined molecular ion features with two, three, and four ions with isotopic resolution, due to Ag44(SePh)30. Additional characterization with diverse tools confirmed the composition. The closed-shell 18 electron superatom electronic structure, analogous to Ag44(SR)30 stabilizes the dodecahedral cage with a large HOMO-LUMO gap of 0.71 eV. The time-dependent density functional theory (TDDFT) prediction of the optical absorption spectrum, assuming the Ag44(SR)30 structure, matches the experimental data, confirming the structure.
Effects of Interleukin-1 Blockers on Ophthalmic Wound Healing in a Rabbit Model of Trabeculectomy (1995)

A trabeculectomy model was used to test the efficacy of some Interleukin-1 blockers on the modulation of ophthalmic wound healing in the pigmented rabbit. The corticosteroid, methyl prednisolone, was used as a positive control and was effective in prolonging the time to failure of the trabeculectomy by 21%. The experimental compounds CK-17, CK-101A, CK-102 and CK-103A were more efficacious than vehicle control at prolonging the number of days before failure of the trabeculectomy by 55%, 55%, 30% and 79%, respectively. The CK- compounds increased the amount of time before failure of the fistula, with no side effects at the doses tested to date.
Pyocin inhibition of Neisseria gonorrhoeae: mechanism of action (1980)

Purified R-type pyocins (611 131) from Pseudomonas aeruginosa PA103 exhibited bactericidal activity against Neisseria gonorrhoeae. Killing of gonococci was a single-hit process requiring as few as 1 pyocin per colony-forming unit. Deoxyriboinucleic acid, ribonucleic acid, protein, and lipid syntheses were rapidly and completely inhibited. Oxygen uptake was also inhibited, but occurred after the inhibition of macromolecular synthesis. The cell lysis which occurred after pyocin inhibition of gonococcal growth was the result of endogenous gonococcal autolysin activity.
Evaluation of Lactic Acid Bacteria for the Resistance to Endocrine Disruptors (2002)

Endocrine disruptors are chemicals which can be found in our normal daily life. They can be easily ingested through plastic food containers, pesticides, etc. They include DDT, bisphenol A, benzophenone and phenylphenol, etc. Endocrine disrupter can be very harmful and toxic because it disrupts the normal function of the endogenous endocrine system. It has been reported that endocrine disruptor can cause the fatal strike in reproductive system, central nervous system and the other part of the body. We have examined if the growth of lactic acid bacteria could be resistant to the endocrine disruptor. We have used Lactobacillus delbruekii as an experimental strain and benzophenone and phenylphenol for the comparison purpose. Experiments included the evaluation of turbidity, absorbance and actual cell counts. Although Lactobacillus delbruekii showed the higher resistance to benzophenone than phenylphenol it was still resistant to both benzophenone and phenylphenol. Because the experimental concentrations of benzophenone and phenylphenol were so high to compare with the actual concentration we meet in daily life, Lactobacillus delbruekii was considered to be sufficient to survive in the environmental concentration of these endocrine disruptors. This study should contribute to the development of fermented beverage with beneficial effect by lactic acid bacteria.
Sequential high-dose methotrexate and fluorouracil combined with doxorubicin--a step ahead in the treatment of advanced gastric cancer: a trial of the European Organization for Research and Treatment of Cancer Gastrointestinal Tract Cooperative Group. (1991)

In a prospective phase III multicenter trial, 213 patients with advanced measurable or nonmeasurable gastric cancer were randomized to receive methotrexate (MTX), fluorouracil (5-FU), and Adriamycin (doxorubicin; Farmitalia Carlo Erba, Milan, Italy) (FAMTX) or 5-FU, Adriamycin, and mitomycin (FAM). The results show a significantly superior response rate (41% v 9% [P less than .0001]), and survival (median, 42 weeks v 29 weeks [P = .004]) for FAMTX. There was a cumulative thrombocytopenia in FAM and not in FAMTX. The FAMTX protocol should be the reference treatment in future clinical trials that seek to improve the therapeutic outcome in advanced gastric cancer.
Capillary Electrophoretic Enzyme Immunoassay for Digoxin in Human Serum (1995)

The combined use of capillary electrophoretic (CE) separation and homogeneous enzyme immunoassay for analyzing drugs in hemolyzed, lipemic, or icteric serum samples was investigated. An FDA-approved EMIT assay kit for digoxin in human serum was used. After the enzyme immunoassay, the enzymatic reaction product (NADH) and remaining substrate (NAD+), together with internal standard (p-nitrophenol, NP), were electrokinetically injected into a polyacrylamide-coated electrophoresis capillary and separated under applied potential. Detection was made by monitoring the UV adsorption at wavelength of 260 nm. The digoxin level in human serum was determined by comparing the peak area ratio of NADH and NP to the ratios established by the known digoxin standards. In this study, the factors that influence the CE separation were also investigated. Under the optimum conditions, NADH, NAD+, and NP were separated at electric field strength of 438 V/cm in the coated capillary (100 microns x 57 cm) with 200 mM Tris-borate buffer (pH 7.9) containing 0.2% hydroxypropyl methylcellulose. CE analyses of serum samples spiked with NADH standards at concentrations of 100 and 400 microM resulted in detection variabilities of less than 2% and analytical recoveries of 98-102%. Both an internal calibration plot for NADH and a dose-response curve for digoxin in serum were constructed. Calibrator serum, patients' sera with hemolyzed, lipemic, and icteric interference factors, and other pigmented blood components (e.g., serum albumin, bilirubin, hemoglobin, uric acid, coproporphyrin, melanin, protoporphyrin IX, and uroprophyrin) demonstrated no interference in this method. The authors believed that this method is useful for analyzing digoxin in hemolyzed, lipemic, and icteric blood samples that are known to create problems in conventional EMIT assays and may be applicable to other EMIT-based assays for monitoring drugs in complex biological matrices.
Visible light communications in intelligent transportation systems (2012)

Emerging intelligent transportation systems (ITS) are based on several technologies. A new concept of integrating visible light communications (VLC) in ITS is introduced in this paper. VLC in ITS is a cost effective method of implementation. This paper presents a VLC broadcast system considering LED-based traffic lights. It discusses the integration of traffic light road side unit (RSUs) within the existing ITS architecture. A prototype demonstrator of the designed VLC systems is also presented. A robust modulation technique based on direct sequence spread spectrum (DSSS) sequence inverse keying (SIK) has been implemented to minimize the effect of noise sources. Experimental results show data communication range of over 40m with 200mm custom designed traffic light, even during bright sun light.
Miniaturized fiber‐optic transmission system for MRI signals (2007)

Conventional MRI instruments transmit received MRI signals through electrical cables. Although this design has proved to be effective over the years, we report a fiber-optic system that addresses the needs of recent developments in MRI technology. One of these technologies is phased array coils with a high number of elements, where total size of interconnections is a primary problem, and other problem is internal MRI coils, where there is a need for improvements in safety. The Miniature Fiber-Optic Transmission (FOT) System was developed to address these issues. The system consists of a receiver coil with active detuning, a low-noise preamplifier, and a laser diode connected to a photodetector with fiber-optic cabling. The overall noise figure of the system is lower than 1 dB. Total power consumption is 50 mW, and the device is switchable with another fiber-optic line, which can also control active detuning. A prototype device was tested in a GE 1.5 Tesla MRI scanner, and several images were acquired with a signal to noise ratio similar to coaxial cabling. We believe that this design will reduce the cabling problems of arrays and enable placement of internal coils into body cavities with no safety hazard to the patient, such as electrical shock or burns.
Distinct patterns of short-chain fatty acids during flare in patients with ulcerative colitis under treatment with mesalamine or a herbal combination of myrrh, chamomile flowers, and coffee charcoal: secondary analysis of a randomized controlled trial (2019)

Patients who received the herbal preparation did not show a significant decline of total short-chain fatty acids in the event of a flare. Since a decline of short-chain fatty acids might lead to unfavorable health impairments, a combination of the two treatments should be further investigated.
Ink-jet printing of polymers ? from single dots to thin film libraries (2004)

Recent results on the ink-jet printing of polymer films are presented. Films that were printed using an ink-jet process based on one single solvent always suffered from ring formation, i.e. the coffee-drop effect. This drawback could be overcome by the use of solvent mixtures. The effects of solvent mass ratio, print head velocity, dot spacing and printing method were investigated and optimized to reproducibly obtain homogeneous polymer films (6 × 6 mm size). The results obtained represent an important step towards the application of ink-jet printing for the controlled deposition of polymer dots and libraries.
A Randomized, Placebo-Controlled Trial of Zoledronic Acid in Patients With Hormone-Refractory Metastatic Prostate Carcinoma (2002)

Zoledronic acid at 4 mg reduced skeletal-related events in prostate cancer patients with bone metastases.
<i>Mas</i> Deficiency in FVB/N Mice Produces Marked Changes in Lipid and Glycemic Metabolism (2007)

These results show that Mas deficiency in FVB/N mice leads to dramatic changes in glucose and lipid metabolisms, inducing a metabolic syndrome-like state.
Pharmacokinetic and pharmacodynamic modeling of the effects of oral and intravenous administrations of dofetilide on ventricular repolarization* (1995)

We conclude that dofetilide prolongs QTc interval duration in a concentration-dependent manner in normal volunteers during sinus rhythm and that pharmacokinetic-pharmacodynamic modeling is useful for examination of maximum QTc prolongation induced by dofetilide.
Individual Identification Using Images of Eyes (1997)

Individual identification is required in various fields such as credit business, security business, information industry and crime investigation. This paper describes the individual identification system using images of eyes. With this system having used images data of 20 registered persons and 20 unregistered persons, we could obtain a high recogniniton rate and showing how efficient this system is.