gebhart/patrepeval huggingface dataset:
Human leukocyte function‐associated antigens on lympho‐hemopoietic precursor cells (1986)

The expression of human leukocyte function-associated antigens (HLFA, equivalents of murine LFA-1) was studied on early lympho-hemopoietic cells in infant thymus and normal bone marrow by double immunofluorescence methods, cell sorting and colony-forming assays. Monoclonal antibodies MHM-24 recognizing the alpha chain (180 kDa) and 60.3 and 60.1 identifying the beta chain (95 kDa) were used. The vast majority of thymocytes including large terminal deoxynucleotidyl transferase (TdT)-positive blast cells are HLFA positive. On the other hand, most B cell precursors in the bone marrow, identified by the expression of nuclear TdT, do not show surface HLFA which appears at the pre-B cell stage (cytoplasmic mu positive, surface Ig negative). Cell sorting experiments revealed an enrichment of early myeloid cells (myeloblasts and colony-forming unit cells) in the HLFA-negative fraction. Erythroid cells appeared to be completely negative from the burst-forming unit erythrocyte stage onwards. Anti-HLFA-antibodies, capable of blocking T cell and natural killer function, may have therapeutic potential without interfering with precursor cell development in man.
Photoreceptor Protection after Photodynamic Therapy Using Dexamethasone in a Rat Model of Choroidal Neovascularization (2008)

Systemic administration of dexamethasone combined with PDT reduces photoreceptor apoptosis, increases angiographic closure, and reduces CNV size compared with PDT alone in a rat model.
Development of a Carbon Film Electrode Ferrocene‐Mediated Glucose Biosensor (2005)

Abstract An amperometric ferrocene‐mediated glucose biosensor was developed using the cross‐linking method with glutaraldehyde and bovine serum albumin (BSA) on carbon film electrodes fabricated by pyrolytic deposition of carbon. Ferrocene was mixed with the enzyme mixture or incorporated afterwards by potential cycling; the addition of Nafion to the mixture or coating the biosensor assembly with Nafion was also tested. The optimized operating potential for ferrocene‐mediated catalysis of the generated hydrogen peroxide was 0.25 V vs. Ag/AgCl, an improvement on sensors reported in the literature. The best assembly in terms of sensitivity and detection limit of 66 µM was found to be one using an external Nafion coating. Interferences were generally small. This sensor was applied to the determination of glucose in sweet wine samples by the standard addition method. Financial support from Fundação para a Ciência e Tecnologia (FCT), ICEMS (Research Unit 103) and European Project HPRN‐CT‐2002‐00186 is gratefully acknowledged. M.E.G. thanks FCT for a Ph.D. grant (SFRH/BD/14014/2003). Prof. H.‐D. Liess is thanked for the gift of the electrical resistors. Keywords: Amperometric glucose sensorferrocenecarbon film electrodeNafion Notes Financial support from Fundação para a Ciência e Tecnologia (FCT), ICEMS (Research Unit 103) and European Project HPRN‐CT‐2002‐00186 is gratefully acknowledged. M.E.G. thanks FCT for a Ph.D. grant (SFRH/BD/14014/2003). Prof. H.‐D. Liess is thanked for the gift of the electrical resistors.
Mechanism of Inorganic Phosphate Interaction with Phosphate Binding Protein from<i>Escherichia coli</i> (1998)

The mechanism of Pi interaction with phosphate binding protein of Escherichia coli has been investigated using the A197C mutant protein labeled with a coumarin fluorophore (MDCC-PBP), which gives a fluorescence change on binding Pi. A pure preparation of MDCC-PBP was obtained, in which the only significant inhomogeneity is the presence of equal amounts of two diastereoisomers due to the chiral center formed on reaction of the cysteine with the maleimide. These diastereoisomers could not be separated, but Pi binding data suggest that they differ in affinity and fluorescence change. When Pi binds to MDCC-PBP, the fluorescence quantum yield increases 8-fold and the fluorescence intensity at 465 nm increases 13-fold. The kinetics of Pi binding show saturation of the rate at high Pi concentrations, and this together with other information suggests a two-step mechanism with the fluorescence change after binding, concomitant with a conformational change of the protein that closes the cleft containing the Pi binding site. Cleft closure has a rate constant of 317 s-1 (pH 7.0, 5 degrees C), and opening has a rate constant of 4.5 s-1. The fluorescence increase is likely to arise from a change in the hydrophobic environment during this closure as the steady state fluorescence emission (lambdamax and intensity) on Pi binding is mimicked by the addition of ethanol to aqueous solutions of an MDCC-thiol adduct. Fluorescence lifetimes in the absence and presence of Pi were 0.3 and 2.4 ns, respectively, consistent with the change in quantum yield. The rotational correlation time of the coumarin increases only 2-fold from 15 to 26 ns on binding Pi as measured by time-resolved polarization, consistent with the main rotation being determined by the protein even in the open conformation, but with greater local motion. Circular dichroism of the coumarin induced by the protein is weak in the absence of Pi and increases strongly upon saturation by Pi. These data are also consistent with an open to closed conformational model.
Mathematics and Statistics for use in Pharmacy, Biology and Chemistry. By L. Saunders and R. Fleming. Pp. x, 257. 27s. 6d. 1957. (The Pharmaceutical Press) (1959)

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Expression and activation of protein kinase C isoforms in a human megakaryocytic cell line. (1996)

Megakaryocytes undergo a unique differentiation program, becoming polyploid through repeated cycles of DNA synthesis without concomitant cell division. We have shown previously that phorbol 12-myristate 13-acetate (PMA) induces the Dami human megakaryocytic cell line to become polyploid and to express platelet-specific proteins, including von Willebrand factor (vWF) and glycoprotein Ib (GpIb). Phorbol esters are thought to regulate gene expression principally through the activation of protein kinase C (PKC), a family of structurally related kinases with potentially unique activation requirements and substrate specificities. A survey of PKC isoforms in Dami cells revealed that, by both Western and Northern analyses, PKC isoforms alpha, beta, delta, epsilon, eta, theta, and zeta were reproducibly detected. PKC-gamma was not detected. In order to define the role of individual PKC isoforms in megakaryocytic maturation, PMA and 2-deoxyphorbol 13-phenylacetate 20-acetate (dPPA), a putative selective activator of the PKC-beta 1 isotype, were compared for their effects on Dami cell maturation. Treatment with either dPPA or PMA caused Dami cells to cease proliferating, to become polyploid, and to express vWF. We also examined dPPA and PMA for their ability to activate and to downregulate expression of different PKC isoforms. Fifteen-minute treatment with PMA resulted in the translocation of PKC isoforms alpha, epsilon, and theta from the cytosolic to the membrane fraction; twenty-four hour treatment resulted in the downregulation of these isoforms. In contrast, dPPA was found to be a potent activator of PKC-epsilon alone and exhibited weaker effects on alpha and theta. These data suggest that PKC isoforms beta, delta, eta, and zeta, which appear not to be activated by either phorbol ester, are unlikely to be primarily involved in megakaryocytic maturation in response to these agents. The isoforms that are translocated by both phorbol esters-PKC isoforms alpha and theta, and particularly epsilon-are more likely to transduce the signals that stimulate Dami cell differentiation.
Continuous metal-insulator transition in the pyrochlore<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Cd</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">Os</mml:mi></mml:mrow><mml:mrow><mml:mn>2</mml:mn></mml:mrow></mml:msub></mml:mrow><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">O</mml:mi></mml:mrow><mml:mrow><mml:mn>7</mml:mn></mml:mrow></mml:msub></mml:mrow></mml:math> (2001)

${\mathrm{Cd}}_{2}{\mathrm{Os}}_{2}{\mathrm{O}}_{7}$ crystallizes in the pyrochlore structure and undergoes a metal-insulator transition (MIT) near 226 K. We have characterized the MIT in ${\mathrm{Cd}}_{2}{\mathrm{Os}}_{2}{\mathrm{O}}_{7}$ using x-ray diffraction, resistivity at ambient and high pressure, specific heat, magnetization, thermopower, Hall coefficient, and thermal conductivity. Both single crystals and polycrystalline material were examined. The MIT is accompanied by no change in crystal symmetry and a change in unit-cell volume of less than 0.05%. The resistivity shows little temperature dependence above 226 K, but increases by 3 orders of magnitude as the sample is cooled to 4 K. The specific heat anomaly resembles a mean-field transition and shows no hysteresis or latent heat. ${\mathrm{Cd}}_{2}{\mathrm{Os}}_{2}{\mathrm{O}}_{7}$ orders magnetically at the MIT. The magnetization data are consistent with antiferromagnetic order, with a small parasitic ferromagnetic component. The Hall and Seebeck coefficients are consistent with a semiconducting gap opening at the Fermi energy at the MIT. We have also performed electronic structure calculations on ${\mathrm{Cd}}_{2}{\mathrm{Os}}_{2}{\mathrm{O}}_{7}.$ These calculations indicate that ${\mathrm{Cd}}_{2}{\mathrm{Os}}_{2}{\mathrm{O}}_{7}$ is metallic, with a sharp peak in the density of states at the Fermi energy. We interpret the data in terms of a Slater transition. In this scenario, the MIT is produced by a doubling of the unit cell due to the establishment of antiferromagnetic order. A Slater transition---unlike a Mott transition---is predicted to be continuous, with a semiconducting energy gap opening much like a BCS gap as the material is cooled below ${T}_{\mathrm{MIT}}.$
Objective evaluation of fatigue by EEG spectral analysis in steady-state visual evoked potential-based brain-computer interfaces (2014)

The experiment results demonstrate the feasibility and effectiveness of the proposed method as an objective and real-time evaluation of the fatigue in SSVEP-based BCIs. This method would be helpful in understanding the fatigue problem and optimizing the system design to alleviate the fatigue in SSVEP-based BCIs.
Somatic Cell Genetics of Human Interferon Production in Human-Rodent Cell Hybrids (1979)

Forty-two primary human-mouse cell hybrids, derived in two separate experiments, were treated with Newcastle disease virus (NDV): eight hybrids were found to produce human interferon and this was shown in every case to be predominantly of the fibroblast type. An extensive analysis was made in terms of karyotype and marker enzymes on all the eight hybrids producing interferon and also on five hybrids which did not produce interferon, five randomly selected hybrids and eleven subclones resistant to diphtheria toxin. The results suggest that, contrary to previous reports, a gene on chromosome 5 is not involved in production of human interferon. Its production was however correlated with the presence of chromosome 9 in the hybrids. Analyses of two sets of human-Chinese hamster hybrid subclones from two different crosses were also consistent with the assignment of a human interferon gene to chromosome 9.
Immunohistochemical localization of placental-like alkaline phosphatase in testis and germ-cell tumors using monoclonal antibodies. (1983)

Six monoclonal antibodies raised against the human placental alkaline phosphatase (ALP) recognizing distinct antigenic determinants on the surface of this isozyme were used for immunohistochemical studies of adult and fetal human testes and testicular germ-cell tumors. ALP reacting with all six antibodies was defined as placental, whereas ALP reacting with some but not all antibodies was labeled as placental-like. ALP reacting with one of the monoclonal antibodies that recognizes a determinant common to intestinal and placental ALP was tentatively considered probably intestinal, unless it reacted with any other monoclonal placental specific antibody. Using this approach, the authors have identified placental ALP in 4 of 7 seminomas, 3 of 7 tumors composed in part or fully of embryonal carcinoma, and 1 yolk sac carcinoma. Placental-like ALP was identified in 2 additional seminomas and 4 embryonal carcinoma-containing tumors, whereas 1 seminoma and 1 benign teratoma were devoid of either placental or placental-like ALP. Trophoblastic giant cells in 2 seminomas and 3 teratocarcinomas expressed only the antigenic determinant common to placental and intestinal ALP. The authors thus show that testicular tumor cells may express either placental or placental-like ALP and that in some instances, the tumor isozyme is antigenically different from ALP found on either fetal or adult testicular germ cells.
Molecular genetics in basic myology: A rapidly evolving perspective (1988)

Myology has greatly benefited from the recent unification of concepts in molecular, cellular, and developmental biology. The interplay between intrinsic and extrinsic factors in determining the physiologic characteristics of individual myofibers has emerged as an important theme. Of special note is the manner in which the study of contractile protein gene structure and expression has contributed to our understanding of the development and ultimate plasticity of the contractile apparatus. As mechanistic models of normal myogenesis achieve increasing sophistication, the opportunities for understanding the pathogenesis of progressive muscle disfunction improve. In this article we review recent progress in basic myology which will be of interest to clinicians studying the heritable neuromuscular disorders.
Reflections on Teaching System Dynamics Modeling to Secondary School Students for over 20 Years (2018)

This paper contains the description of a successful system dynamics (SD) modeling approach used for almost a quarter-century in secondary schools, both in algebra classes and in a year-long SD modeling course. Secondary school students have demonstrated an ability to build original models from the news, write technical papers explaining their models, and present a newfound understanding of dynamic feedback behavior to an audience. The educational learning theory and instructional methods used for both the algebra and modeling courses are detailed, with examples. Successful student SD modeling experiences suggest the SD approach can expand the sophistication of topics that secondary school students can understand.
Design and modeling of microstrip-microslot applicators with several patches and apertures for microwave hyperthermia (1997)

This article describes external microstrip-microslot applicators with several patches radiating through one or several apertures that have been developed for microwave hyperthermia controlled by microwave radiometry. The possibility of obtaining larger hearing patterns than with the single microstrip-microslot applicators (one patch radiating through one aperture) is clearly focused by the theoretical results that are presented and verified by experimental measurements. © 1997 John Wiley & Sons, Inc.Microwave Opt Technol Lett 14, 121–126, 1997
Summary resettlement action plan (2009)

The objective of the Nanning Urban Environment Project for China is to assist the Nanning Municipality in arresting further deterioration of water quality in selected rivers by expanding coverage of wastewater treatment services, carrying out environmental rehabilitation of river courses, and providing technical assistance for development of the integrated mini-river basin management systems. Negative impacts of the project are: (i) land acquisition; (ii) demolition of residential houses and land attachments; (iii) impacts of demolition of non-residential houses; and (iv) demolition of land attached facilities. Mitigation measures include: 1) compensation shall include compensation for land, resettlement subsidies and attachments and young crops on the acquired land; 2) land compensation shall be owned by collective organization; and crops compensation and resettlement subsidies belong to the land contractor; 3) gain industrial reserved land, in-kind subsidies, project employment, employment training, social insurance for displaced farmers etc ; 4) the compensation for the temporary use of agricultural land shall be calculated as per the multiplier of average annual production value for the past three years before the land is temporally used by the years of temporary use. Necessary crops compensation shall be also paid according to actual situation; 5) house demolition compensation at replacement cost; and 6) the dislocated people shall be exempt from any charges and administration fees involving grievance against resettlement.
Automated wound identification system based on image segmentation and Artificial Neural Networks (2012)

A system that can automatically and accurately identify the region of a chronic wound could largely improve conventional clinical practice for the wound diagnosis and treatment. We designed a system that uses color wound photographs taken from the patients, and is capable of automatic image segmentation and wound region identification. Several commonly used segmentation methods are utilized with their parameters fine-tuned automatically to obtain a collection of candidate wound regions. Two different types of Artificial Neural Networks (ANNs), the Multi-Layer Perceptron (MLP) and the Radial Basis Function (RBF) with parameters determined by a cross-validation approach, are then applied with supervised learning in the prediction procedure for the wound identification, and their results are compared. The satisfactory results obtained by this system make it a promising tool to assist in the field of clinical wound evaluation.
Experimental Autoimmune Myocarditis Produced by Adoptive Transfer of Splenocytes After Myocardial Infarction (1998)

One possible mechanism for neurohumoral activation after myocardial infarction may be the generation of an immune response against cardiac self-antigens. We hypothesize that if there is a T cell-mediated reaction to self-antigens, the transfer of splenic lymphocytes from postinfarct rats into syngeneic rats with normal hearts should result in a T cell-mediated autoimmune myocarditis in the healthy syngeneic rats. Rats were killed 6 weeks after coronary ligation. Splenocytes from animals with large and small infarcts were purified from spleens, activated with concanavalin A, and injected in varying doses into normal syngeneic rats. These recipient rats were killed 6 weeks later, and histopathological studies were performed. Our results demonstrate in vivo evidence of lymphocyte-mediated myocardial injury by adoptive transfer of sensitized lymphocytes from rats who developed congestive heart failure after acute myocardial infarction. The amount of infiltrate and necrosis in the recipient rats appeared directly related to the size of the infarct from the donor rats. This suggests that larger infarcts lead to a greater inflammatory response as well as a greater propensity for alteration of cardiac surface antigens or the emergence of previously sequestered antigens. None of the other organs (kidney, liver, lung, or brain) had evidence of infiltrates. Two-dimensional echocardiography did not reveal systolic dysfunction. This study provides direct evidence of autoimmune myocardial injury produced by adoptive transfer of concanavalin A-activated splenocytes after myocardial infarction. We propose that neurohumoral activation early in the postinfarction period triggers a series of specific inflammatory and immunological events that lead to formation of specific clones of T cells. When these are activated and transferred into normal rats, cardiac-specific cellular infiltration occurs, occasionally accompanied by myocardial necrosis. This model should help to further explore the link between neurohumoral activation after myocardial infarction and the subsequent immune alterations that might be associated with the development and/or progression of congestive heart failure. Additionally, this might be a useful model in which to study other immune-mediated cardiomyopathies.
"Sabbath Is a Happy Day!" What Does Isaiah 58:13-14 Mean? (2002)

As a Seventh-day Adventist who worships on the Sabbath and considers the fourth commandment Gods will for us, Gods call in Isa 58:1314 for us to avoid doing your pleasure on the Sabbath resonates, but what does it mean?I want to do what God asks, but what does He mean by what He asks?Many denominations over the centuries have at times had strict rules against Sabbathbreaking, though theyve defined it in various ways, whether carrying a purse, attending plays, purchasing liquor, or mowing the lawn. 1 Have they been correct in this?Generations of young people have found the Sabbath a burden and moaned about the many pleasures forbidden them on that day. 2 Just how happy a day should Sabbath be?The fourth commandment does not forbid pleasure on Sabbath, but only work.The Hebrew word translated you shall labor, ta{booe d (dO bS oA ; t), is sweat of your brow work, like that done by an {ebed, a servant or slave.The Hebrew word translated your work, m la}kttekaoe ( KR ; tV kaA lV m), especially suggests occupations, such as shopkeeper or craftsman, the work of commerce, though it also means all work. 3The Old Testament says very little about Sabbath worship, but it strongly emphasizes Sabbath rest.The Hebrew word for Sabbath, s abbat (tD ; bA vA h) is a noun.The verb it is derived from, s abat (tA bD v), means to cease, primarily from work.While liberal and secular scholars tend to doubt that Isaiah had anything to do with Isa 58, I do not find their arguments convincing, and it seems irrelevant 1 See, for example, the tractate Shabbat in the Mishnah. 2 I dedicate this article to my thirteen-year-old son Paul, whose frequent question, Whats wrong with doing it on Sabbath? encouraged me to search the Scriptures.
Randomized comparison of the effects of tamoxifen, megestrol acetate, or tamoxifen plus megestrol acetate on treatment response and survival in patients with metastatic breast cancer (1993)

Summary Background The antioestrogen tamoxifen and progestins act via different receptors and may therefore have complementary effects against human breast cancer. This possibility was tested in a randomized study which compared the effects of tamoxifen, standard-dose megestrol acetate, and these two agents in combination, in patients with metastatic breast cancer. Patients and methods 184 post-menopausal patients with metastatic breast cancer were randomized to initial treatment with either tamoxifen (TAM) 40 mg daily, megestrol acetate (MA) 160 mgm daily, or the combination of the two administered simultaneously. Patients crossed over to the alternative single agent on relapse or disease progression. Patients were evaluated for response, time to initial and ultimate treatment failure, and survival. Results There were no significant differences between the three groups with respect to response rates, nor the other parameters. Patient survival was significantly associated with age >60 years, ER positive status, and the absence of visceral metastases. Conclusions TAM and MA are both equally effective in response induction as initial treatments and the combination has no advantage. Sequential treatment is still optimal, TAM being the preferred initial agent in view of the reported side effects with MA.