gebhart/patrepeval huggingface dataset:
The binding pattern of two carbohydrate-binding modules of laminarinase Lam16A from Thermotoga neapolitana: differences in β-glucan binding within family CBM4 (2001)

Carbohydrate-binding modules (CBMs) are often part of the complex hydrolytic extracellular enzymes from bacteria and may modulate their catalytic activity. The thermostable catalytic domain of laminarinase Lam16A from Thermotoga neapolitana (glycosyl hydrolase family 16) is flanked by two CBMs, 148 and 161 aa long. They share a sequence identity of 30%, are homologous to family CBM4 and are thus called CBM4-1 and CBM4-2 respectively. Recombinant Lam16A proteins deleted for one or both binding modules and the isolated module CBM4-1 were characterized. Proteins containing the N-terminal module CBM4-1 bound to the soluble polysaccharides laminarin (1,3-beta-glucan) and barley 1,3/1,4-beta-glucan, and proteins containing the C-terminal module CBM4-2 bound additionally to curdlan (1,3-beta-glucan) and pustulan (1,6-beta-glucan), and to insoluble yeast cell wall beta-glucan. The activity of the catalytic domain on soluble 1,3-beta-glucans was stimulated by the presence of CBM4-1, whereas the presence of CBM4-2 enhanced the Lam16A activity towards gelatinized and insoluble or mixed-linkage 1,3-beta-glucan. Thermostability of the catalytic domain was not affected by the truncations. Members of family CBM4 can be divided into four subfamilies, members of which show different polysaccharide-binding specificities corresponding to the catalytic specificities of the associated hydrolytic domains.
Reversed‐phase monoliths prepared by UV polymerization of divinylbenzene (2011)

Many different techniques have been developed to prepare monolithic materials specifically for chromatographic techniques. The two most popular polymerization techniques being thermal or via ultra violet (UV) light. Whereas thermal polymerization is easily employed for a whole variety of monomer and porogen systems, UV polymerization has been limited to methacrylate-based systems, and styrenic systems have been avoided due to their strong absorbance at low wavelengths. By careful consideration of wavelength, initiator and other system components, it was proven that reversed-phase columns for the separation of proteins and peptides can be prepared using divinylbenzene through UV initiation of 2-methyl-4'-(methylthio)-2-morpholinopropiophenone at a wavelength of 350 nm.
Small-Area Estimation of Economic Statistics (1990)

A ratio-correlation (multiple regression) approach for estimating key economic statistics for small areas is proposed and compared with several synthetic estimation methods using various measures of performance. Using published data that are easily accessible, the methods provide a means of estimating statistics not currently published with a reasonable amount of error.
Posttranslational regulation of nitrogenase in Rhodospirillum rubrum strains overexpressing the regulatory enzymes dinitrogenase reductase ADP-ribosyltransferase and dinitrogenase reductase activating glycohydrolase (1995)

Rhodospirillum rubrum strains that overexpress the enzymes involved in posttranslational nitrogenase regulation, dinitrogenase reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase activating glycohydrolase (DRAG), were constructed, and the effect of this overexpression on in vivo DRAT and DRAG regulation was investigated. Broad-host-range plasmid constructs containing a fusion of the R. rubrum nifH promoter and translation initiation sequences to the second codon of draT, the first gene of the dra operon, were constructed. Overexpression plasmid constructs which overexpressed (i) only functional DRAT, (ii) only functional DRAG and presumably the putative downstream open reading frame (ORF)-encoded protein, or (iii) all three proteins were generated and introduced into wild-type R. rubrum. Overexpression of DRAT still allowed proper regulation of nitrogenase activity, with ADP-ribosylation of dinitrogenase reductase by DRAT occurring only upon dark or ammonium stimuli, suggesting that DRAT is still regulated upon overexpression. However, overexpression of DRAG and the downstream ORF altered nitrogenase regulation such that dinitrogenase reductase did not accumulate in the ADP-ribosylated form under inactivation conditions, suggesting that DRAG was constitutively active and that therefore DRAG regulation is altered upon overexpression. Proper DRAG regulation was observed in a strain overexpressing DRAT, DRAG, and the downstream ORF, suggesting that a proper balance of DRAT and DRAG levels is required for proper DRAG regulation.
Distributing US Health Aid (2009)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA HomeNew OnlineCurrent IssueFor Authors Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) Podcasts Clinical Reviews Editors' Summary Medical News Author Interviews More JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA journal
The Maillard Reaction for Sunlight Protection (2005)

During seven months of a clinical trial in spring, summer, and fall, 30 UVA/B/Soret band-photosensitive patients used sequential topical applications of dihydroxyacetone (DHA) followed by naphthoquinone only at bedtime and received excellent photoprotection without a single therapeutic failure or loss of any patient to follow-up. Eighteen of the 30 patients extended the limits of their photoprotection repeatedly over a seven-month period to tolerate without sunburns six to eight hours of midday sunlight under all kinds of occupational and recreational environmental conditions. Previously, the use of 3% DHA topically in earlier studies gave only a sun protection factor (SPF) of 3. In this reanalysis of the original notes of a previous clinical study of the melanoidins produced by DHA followed by naphthoquinone in the keratin layers of the epidermis of minimally pigmented Caucasian photosensitive patients, it is determined that these patients received a minimal UVB photoprotection of SPF 18 or more. This represents at least a sixfold amplification of the UVB photoprotective effect over the use of only dihydroxyacetone in the Maillard reaction.
Effects of vasopressin antagonist on vasopressin binding, adenylate cyclase activation, and water flux. (1985)

We studied the effect of an arginine vasopressin (AVP) analogue, (1-[beta-mercapto-beta, beta-cyclopentamethylenepropionic acid],2-O-ethyltyrosine, 4-valine)AVP(d[CH2]5Tyr[Et]VAVP), on the stimulation of adenylate cyclase by various hormones in the isolated nephron segments and 3H-AVP binding to renal papillary membranes from the rat. The net water flux across the renal cortical collecting tubules of the rabbit was also examined. We found that d(CH2)5Tyr(Et)VAVP significantly inhibited adenylate cyclase activation by AVP in cortical, medullary, and papillary collecting tubules and in the medullary thick ascending limb. In contrast, the AVP analogue did not alter the stimulation of adenylate cyclase by parathyroid hormone in the cortical thick ascending limb, by glucagon in the medullary thick ascending limb, and by calcitonin in cortical collecting tubules. In addition, d(CH2)5Tyr(Et)VAVP blocked [3H]AVP binding to renal papillary membranes. The enhanced net water transport induced by AVP in isolated, perfused rabbit cortical collecting tubules also was completely blocked by this AVP analogue. These results indicate that d(CH2)5Tyr(Et)VAVP specifically antagonizes the cellular action of AVP on the medullary thick ascending limb and on the cortical, medullary, and papillary collecting tubules. Evidence is also presented for competitive antagonism as the cellular mechanism of action.
The risk management system as a necessary factor for Innovation Company (2017)

Innovation activity of companies takes place in conditions of high uncertainty and is associated with high risks. Therefore, under current conditions, a comprehensive and complete system of risk management is becoming an indispensable tool company, providing measures for the entire spectrum of threats activity. In this regard, a detailed analysis of the risk management system of elements for example a gas company. The necessity of integrating risk management into the overall enterprise management system as the main instrument to minimize the risk and consequences of risk situations, as well as for the successful implementation of innovation in general, and enhance the competitiveness of enterprises. It proposed to conduct its own risk register for the largest innovative companies.
Weld strength and crack growth ductility from the Lazy-L test. (1995)

Fully plastic fracture of fillet welded T-joints is characterized by the limit moment, slip line displacement to crack initiation, and crack growth ductility, defined as the slip line displacement per unit crack advance. A novel fillet weld fracture experiment, the Lazy-L Test, is developed as a simple, inexpensive method of measuring data used to calculate these parameters. Fully plastic fracture mechanics provides an extrapolation of Lazy-L Test results to other fillet weld failure modes observed in ship grounding, including peeling of the shell plating. The Lazy-L Test is introduced as an alternative to more costly and difficult experimental and computer methods, and suggested for consideration as a standard test in the design and qualification of fillet welds for service. The Lazy-L is used to test single fillets in predominant bending (opening from the root of the weld), single fillets in predominant transverse shear, and double fillets in predominant bending The plastic flow field for each configuration is modeled by single arcs of sliding in the fillet selected to yield a least upper bound to the limit moment. Load-displacement data for each configuration determine an experimental limit moment,
Design and implementation of anti-theft ATM machine using embedded systems (2015)

Automated Teller Machines (ATMs) security is the field of study that aims at solutions that provide multiple points of protection against physical and electronic theft from ATMs and protecting their installations. From anti-skimming defend systems to silent indicate systems, integrated ATM video surveillance cameras and ATM monitoring options, security specialists are ready to help the people get more out of the ATM security and ATM loss prevention systems. The implementation is achieved with the use of Machine-to-machine (M2M) communications technology. M2M communications is a topic that has recently attracted much attention It provides real-time monitoring and control without the need for human intervention. The idea of M2M platform suggests new system architecture for positioning and monitoring applications with wider coverage and higher communication efficiency. The aim of the proposed work is to implement a low cost stand-alone Embedded Web Server (EWS) based on ARM11 processor and Linux operating system using Raspberry Pi. It offers a robust networking solution with wide range of application areas over internet. The Web server can be run on an embedded system having limited resources to serve embedded web page to a web browser. The setup is proposed for ATM security, comprising of the modules namely, authentication of shutter lock, web enabled control, sensors and camera control.
Tumor Cell Targeting by Iron Oxide Nanoparticles Is Dominated by Different Factors In Vitro versus In Vivo (2015)

Realizing the full potential of iron oxide nanoparticles (IONP) for cancer diagnosis and therapy requires selective tumor cell accumulation. Here, we report a systematic analysis of two key determinants for IONP homing to human breast cancers: (i) particle size and (ii) active vs passive targeting. In vitro, molecular targeting to the HER2 receptor was the dominant factor driving cancer cell association. In contrast, size was found to be the key determinant of tumor accumulation in vivo, where molecular targeting increased tumor tissue concentrations for 30 nm but not 100 nm IONP. Similar to the in vitro results, PEGylation did not influence in vivo IONP biodistribution. Thus, the results reported here indicate that the in vitro advantages of molecular targeting may not consistently extend to pre-clinical in vivo settings. These observations may have important implications for the design and clinical translation of advanced, multifunctional, IONP platforms.
Pyridines in foods (1981)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPyridines in foodsJoseph A. MagaCite this: J. Agric. Food Chem. 1981, 29, 5, 895–898Publication Date (Print):September 1, 1981Publication History Published online1 May 2002Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/jf00107a001https://doi.org/10.1021/jf00107a001research-articleACS PublicationsRequest reuse permissionsArticle Views473Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A Vehicle Detection Technique Using Binary Images for Heterogeneous and Lane-Less Traffic (2021)

Nowadays, providing a low-cost traffic management system in developing countries or in heterogeneous and lane-less traffic conditions is highly essential. It can help to manage traffic congestion, save fuel, save travel time, and enhance user safety. By keeping these as an objective, this article presents a vehicle detection method in heterogeneous and lane-less traffic by extracting a <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">binary image</i> from a discrete sensor array. The <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">binary image</i> is formed with a logic 1 or 0, which are recorded based on the occupancy status of the vehicles in an observed zone. The proposed method is demonstrated with virtual loops in video and with an array of micro-LiDARs. The width and length of the vehicle are obtained from the <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">binary image</i> , which is extracted from virtual loops in a video recording and classified the vehicles. Similarly, the width and height information is obtained using an array of micro-LiDARs and classified the vehicles. The proposed method can easily be implemented with minimal storage, minimum cost, less bandwidth, and less computation complexity than the conventional methods, such as image processing or video processing-based vehicle classification. The proposed classification methods are mathematically derived, implemented, and measured performance over real traffic scenarios. It can be adopted automatically to high or light traffic scenarios by adjusting the distance between observation zones. The detection accuracy of 98% is observed while extracting data from video and 91.3% while using micro-LiDARs. The proposed works are compared with existing techniques.
Loss of the SdhB, but Not the SdhA, Subunit of Complex II Triggers Reactive Oxygen Species-Dependent Hypoxia-Inducible Factor Activation and Tumorigenesis (2007)

Mitochondrial complex II is a tumor suppressor comprised of four subunits (SdhA, SdhB, SdhC, and SdhD). Mutations in any of these should disrupt complex II enzymatic activity, yet defects in SdhA produce bioenergetic deficiency while defects in SdhB, SdhC, or SdhD induce tumor formation. The mechanisms underlying these differences are not known. We show that the inhibition of distal subunits of complex II, either pharmacologically or via RNA interference of SdhB, increases normoxic reactive oxygen species (ROS) production, increases hypoxia-inducible factor alpha (HIF-alpha) stabilization in an ROS-dependent manner, and increases growth rates in vitro and in vivo without affecting hypoxia-mediated activation of HIF-alpha. Proximal pharmacologic inhibition or RNA interference of complex II at SdhA, however, does not increase normoxic ROS production or HIF-alpha stabilization and results in decreased growth rates in vitro and in vivo. Furthermore, the enhanced growth rates resulting from SdhB suppression are inhibited by the suppression of HIF-1alpha and/or HIF-2alpha, indicating that the mechanism of SdhB-induced tumor formation relies upon ROS production and subsequent HIF-alpha activation. Therefore, differences in ROS production, HIF proliferation, and cell proliferation contribute to the differences in tumor phenotype in cells lacking SdhB as opposed to those lacking SdhA.
THE BEHAVIOR OF VIRULENT AND AVIRULENT STAPHYLOCOCCI IN THE TISSUES OF NORMAL MICE (1956)

The fate of hemolytic staphylococci injected intravenousiy into albino mice was followed by determining quantitatively the numbers of living organisms present in the various tissues at different intervals of time after infection. Irrespective of the strain of staphylococcus used, most of the organisms disappeared rapidly from the blood, liver, spleen, and kidneys. This was true even when the infective dose consisted of large numbers of virulent, coagulase-positive staphylococci, capable of producing a fatal disease in a high percentage of the infected mice. The initial rate of removal or destruction of staphylococci was particularly high in the lungs and kidneys. In all cases on the other hand, a few living staphylococci persisted in the various organs for several weeks after infection, even when the organisms were non-virulent and coagulase-negative. Although virulent as well as avirulent staphylococci were eliminated extremely rapidly and efficiently from the kidneys during the initial stage of infection, the microorganisms soon began to multiply in this organ, causing abscesses first detected in the cortex. Death of the animals infected with virulent cultures appeared to be due to the destruction of renal tissue by these abscesses. The abscesses caused by the avirulent strains eventually became sterile, and healed. No convincing difference could be recognized amongst seven strains in their resistance to the bactericidal power of the mouse tissues during the initial phase of the infection. In contrast, marked quantitative differences came to light in their subsequent behavior in the kidneys. The multiplication of the coagulase-negative staphylococci in this organ soon came to an end in all animals and never proceeded far enough to result in fatal disease. The staphylococci of a weakly coagulase-positive strain multiplied somewhat more extensively in the kidneys than did the coagulase-negative, but never sufficiently to cause the death of any animal within the period of observation of 1 month. The three coagulase-positive strains tested yielded the largest bacterial population in the kidneys and caused the death of many of the infected animals. These three virulent strains differed quantitatively amongst themselves with regard to both the rapidity and extent of their multiplication in the kidneys and the lethal power of a given infective dose. Taken together, the findings indicate that the hemolytic strains of staphylococci can be arranged in a continuous spectrum according to their ability to cause disease in albino mice. Although virulence for these animals appeared to be correlated with the production of coagulase, it did not seem to depend upon the ability of this substance to interfere with the bactericidal mechanisms of the mouse organs during the early phase of the infection. Virulence manifested itself chiefly by the production in the kidneys of progressive abscesses originating from the few staphylococci which were not destroyed during the initial bactericidal reaction.
RadHard 16Mbit Monolithic SRAM for Space Applications (2007)

Aeroflex Colorado Springs reported on a new error detection and correction (EDAC) based monolithic 16 Mbit static random access memory (SRAM) at the IEEE Aerospace Conference in 2006 by C. Hafer et al. (2006). This paper is an update on the 16 Mbit SRAM with new proton test data to supplement the heavy ion test data reported in 2006. A review of the 16 Mbit monolithic SRAM includes the SRAM architecture, a product/process description, radiation hardening techniques, electrical performance, total ionizing dose data, single event effects data, and single event upset error-rate calculations. The proton single event upset testing results will be shown at the conference. Since the heavy ion onset LET of the device is around 1 MeV-cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /mg, the device is expected to be sensitive to protons by F.W. Sexton (1992). Proton data, therefore, is critical to a comprehensive understanding of the SEU performance of the device.
Epitaxial growth of GaN/AlN heterostructures (1983)

GaN/AlN heterostructures were grown on sapphire by reactive molecular beam epitaxy. The electrical and luminescent properties of the GaN have been studied. The GaN films on AlN have larger Hall mobilities and show more intense cathodoluminescence at the peak of 360 nm, compared with GaN films grown on sapphire. This suggests that the crystalline quality of the GaN is improved by making GaN/AlN heterostructures. The improvement is thought to result from the small mismatch of lattices and the small difference of thermal expansion coefficients between GaN and AlN.
Boundary Layer Meshing for Viscous Flows in Complex Domains. (1998)

. High reynolds number flow simulations exhibit strong gradients normal to walls and across shear layers requiring much finer resolution of the solution in some directions compared to others. To keep mesh sizes manageable for such problems, meshes with highly anisotropic elements are necessary. In this paper, a method for generating boundary layer meshes for arbitrarily complex non-manifold geometric domains is discussed. A popular strategy for generation of boundary layers meshes is generalized to deal with nonmanifold model topology as well as to generate better shaped elements. Results on complex configurations are presented to demonstrate the capabilities of the mesh generator. 1 Introduction High Reynolds numbers flow simulations exhibit strong gradients near walls and in free shear layers requiring fine resolution of the solution normal to the boundary layer and across the free shear layers. Refining a finite element mesh isotropically to capture strong gradients in some directi...