gebhart/patrepeval huggingface dataset:
Mertk on tumor macrophages is a therapeutic target to prevent tumor recurrence following radiation therapy (2016)

Radiation therapy provides a means to kill large numbers of cancer cells in a controlled location resulting in the release of tumor-specific antigens and endogenous adjuvants. However, by activating pathways involved in apoptotic cell recognition and phagocytosis, irradiated cancer cells engender suppressive phenotypes in macrophages. We demonstrate that the macrophage-specific phagocytic receptor, Mertk is upregulated in macrophages in the tumor following radiation therapy. Ligation of Mertk on macrophages results in anti-inflammatory cytokine responses via NF-kB p50 upregulation, which in turn limits tumor control following radiation therapy. We demonstrate that in immunogenic tumors, loss of Mertk is sufficient to permit tumor cure following radiation therapy. However, in poorly immunogenic tumors, TGFβ inhibition is also required to result in tumor cure following radiation therapy. These data demonstrate that Mertk is a highly specific target whose absence permits tumor control in combination with radiation therapy.
Double Minute Chromosomes in Glioblastoma Multiforme Are Revealed by Precise Reconstruction of Oncogenic Amplicons (2013)

DNA sequencing offers a powerful tool in oncology based on the precise definition of structural rearrangements and copy number in tumor genomes. Here, we describe the development of methods to compute copy number and detect structural variants to locally reconstruct highly rearranged regions of the tumor genome with high precision from standard, short-read, paired-end sequencing datasets. We find that circular assemblies are the most parsimonious explanation for a set of highly amplified tumor regions in a subset of glioblastoma multiforme samples sequenced by The Cancer Genome Atlas (TCGA) consortium, revealing evidence for double minute chromosomes in these tumors. Further, we find that some samples harbor multiple circular amplicons and, in some cases, further rearrangements occurred after the initial amplicon-generating event. Fluorescence in situ hybridization analysis offered an initial confirmation of the presence of double minute chromosomes. Gene content in these assemblies helps identify likely driver oncogenes for these amplicons. RNA-seq data available for one double minute chromosome offered additional support for our local tumor genome assemblies, and identified the birth of a novel exon made possible through rearranged sequences present in the double minute chromosomes. Our method was also useful for analysis of a larger set of glioblastoma multiforme tumors for which exome sequencing data are available, finding evidence for oncogenic double minute chromosomes in more than 20% of clinical specimens examined, a frequency consistent with previous estimates.
Digital planar holography and multiplexer/demultiplexer with discrete dispersion (2003)

Digital Planar Holography (DPH) has arrived due to progress in microlithography, planar waveguide fabrication, and theoretical physics. A computer-generated hologram can be written by microlithography means on the surface of a planar waveguide. DPH combines flexibility of digital holograms, superposition property of volume (thick) holograms, and convenience of microlithographic mass production. DPH is a powerful passive light processor, and could be used to connect multiple optical devices in planar lightwave circuits (PLCs), and if combined with active elements on the same chip, may perform not only analog operations but also logical ones. A DPH implementation of a multiplexer/demultiplexer with discrete dispersion is proposed and demonstrated, avoiding communication signal distortion inherent in multiplexers/demultiplexers with continuous dispersion. The concept of discrete dispersion leads to a device with a flat top transfer function without a loss penalty. The dispersion is created with custom-designed bandgaps for specific directions. A DPH hologram resembles a poly-crystal with long-range correlations, and it exhibits the properties of a quasi-crystal. Unlike photonic crystals, light in quasi-crystal may propagate in almost any direction. Single mode planar waveguides are specially designed to suppress parasitic reflections that appear due to mixture of TE-modes, TM-modes, and cladding modes. Demultiplexers with 2-32 channels were demonstrated on planar waveguides with binary single-layer lithography.
Coronary Blood Flow Produced by Muscle Contractions Induced by Intracardiac Electrical CPR during Ventricular Fibrillation (2009)

Intracardiac applied electrical CPR produced observable skeletal muscle contractions, measurable pressure pulses, and coronary perfusion pressures similar to MCC during a brief episode of untreated VF.
Chorionic gonadotrophin beta subunit mRNA but not luteinising hormone beta subunit mRNA is expressed in the pituitary of the common marmoset (Callithrix jacchus) (2004)

The pituitary gonadotrophins LH and FSH are responsible for regulation of gametogenesis in the testis and ovary. Chorionic gonadotrophin (CG), a third closely related glycoprotein hormone derived by gene duplication of the LHbeta gene and secreted by the placenta in primates, is essential for the rescue of the corpus luteum and maintenance of pregnancy. We have recently shown that marmoset (m) CGbeta mRNA is highly expressed in the pituitary of the common marmoset (Callithrix jacchus) and that LH is less active than human CG in activating the human LH receptor lacking exon 10. To investigate further which gonadotrophin is the actual ligand of the LH receptor (LHR) of the marmoset monkey that naturally lacks exon 10, we identified and characterised the genomic organisation of the mLHbeta gene and its expression. Intergenic PCR amplification of the region encompassing the mLHbeta and the mCGbeta genes revealed that, surprisingly, mCGbeta is located 20 kbp upstream of the LHbeta gene, whereas in other species the intergenic distance is approximately 2-3 kbp. Sequence analysis of the mLHbeta coding region showed 70% identity to mCGbeta and 90% identity to human LHbeta at the amino acid level. Both gonadotrophin beta subunits are present at the genomic level, but RT-PCR of pituitary and placental total RNA using specific oligonucleotides for mCGbeta and mLHbeta showed high expression of mCGbeta mRNA in both tissues, whereas LHbeta was expressed neither in the pituitary nor in the placenta. Thus mLHbeta mRNA is lacking in the marmoset pituitary. Immunohistochemistry of marmoset pituitaries showed that mCG was confined to the gonadotrophes, and partly co-localised in cells stained positively for FSH. Western blot analysis confirmed the presence of mCG in the pituitary. Northern blot analysis using mCGbeta as a probe displayed one transcript of 0.7 kb in the pituitary and detected two transcripts of 1.1 kb and 2 kb in the marmoset placenta. Our results suggest that, in the common marmoset, CG is the only gonadotrophin with luteinising function that is present in the pituitary. We postulate that, owing to an unknown mutational event in evolution, expression of mLH was completely abolished, and CG - which, unlike LH, acts normally even when exon 10 is missing from the LHR - took over its function.
Al<i>x</i>Ga1−<i>x</i>As1−<i>y′</i>P <i>y′</i>–GaAs1−<i>y</i>P<i>y</i> HETEROSTRUCTURE LASER AND LAMP JUNCTIONS (1970)

A method is described to grow successfully from solution AlxGa1−xAs1−y′P y′ (p-type) on GaAs1−yPy (n-type), to preserve the lattice match (y′ ≈ y), and to obtain the improved heterostructure junction devices previously realized only in the AlGaAs/GaAs system. Although not optimized, these structures have been operated as pulsed room-temperature lasers, and because of an inherent wide-gap window can be used conveniently for optical purposes and for excess carrier lifetime measurements. Carrier lifetime measurements, for example, indicate freedom from defects at the AlGaAsP/GaAsP barrier.
Synthesis and Structures of Nickel Halide Complexes Bearing Mono- and Bis-coordinated N-Heterocyclic Carbene Ligands, Catalyzing Grignard Cross-Coupling Reactions (2006)

Substitution of phosphine ligands in nickel(II) halide complexes by now attractive N-heterocyclic carbene (NHC) ligands is one of the well-known organometallic reactions. New, simple, and easy-to-prepare nickel(II) halides bearing both a phosphine and an NHC ligand, [NiX2(PPh3)(NHC)], were synthesized by the reaction of [NiX2(PPh3)2] (X = Cl and Br) with 1 equiv of a bulky NHC ligand. Rather small NHC ligands did not form NHC/PR3 mixed complexes. Controlling the amount of the NHC ligand and purification led to successful isolation in good to moderate yields and structural determination of these carbene complexes. Studies on catalytic Grignard cross-coupling reactions using three complexes, the NHC/PPh3 mixed complex, a “bis”-carbene complex, and [NiCl2(PPh3)2], revealed that the monocarbene complex catalyzes reactions with the highest activity, but, in comparison, catalysis does not proceed well using the latter two complexes.
Transient Tyrosine Phosphorylation of Human Ryanodine Receptor upon T Cell Stimulation (2001)

The ryanodine receptor of Jurkat T lymphocytes was phosphorylated on tyrosine residues upon stimulation of the cells via the T cell receptor/CD3 complex. The tyrosine phosphorylation was transient, reaching a maximum at 2 min, and rapidly declined thereafter. In co-immunoprecipitates of the ryanodine receptor, the tyrosine kinases p56(lck) and p59(fyn) were detected. However, only p59(fyn) associated with the ryanodine receptor in a stimulation-dependent fashion. Both tyrosine kinases, recombinantly expressed as glutathione S-transferase (GST) fusion proteins, phosphorylated the immunoprecipitated ryanodine receptor in vitro. In permeabilized Jurkat T cells, GST-p59(fyn), but not GST-p56(lck), GST-Grb2, or GST alone, significantly and concentration-dependently enhanced Ca(2+) release by cyclic ADP-ribose. The tyrosine kinase inhibitor PP2 specifically blocked the effect of GST-p59(fyn). This indicates that intracellular Ca(2+) release via ryanodine receptors may be modulated by tyrosine phosphorylation during T cell activation.
The T-cell antigen CD5 acts as a receptor and substrate for the protein-tyrosine kinase p56lck. (1994)

CD5 is a T-cell-specific antigen which binds to the B-cell antigen CD72 and acts as a coreceptor in the stimulation of T-cell growth. CD5 associates with the T-cell receptor zeta chain (TcR zeta)/CD3 complex and is rapidly phosphosphorylated on tyrosine residues as a result of TcR zeta/CD3 ligation. However, despite this, the mechanism by which CD5 generates intracellular signals is unclear. In this study, we demonstrate that CD5 is coupled to the protein-tyrosine kinase p56lck and can act as a substrate for p56lck. Coexpression of CD5 with p56lck in the baculovirus expression system resulted in the phosphorylation of CD5 on tyrosine residues. Further, anti-CD5 and anti-p56lck coprecipitated each other in a variety of detergents, including Nonidet P-40 and Triton X-100. Anti-CD5 also precipitated the kinase from various T cells irrespective of the expression of TcR zeta/CD3 or CD4. No binding between p59fyn(T) and CD5 was detected in T cells. The binding of p56lck to CD5 induced a 10- to 15-fold increase in p56lck catalytic activity, as measured by in vitro kinase analysis. In vivo labelling with 32P(i) also showed a four- to fivefold increase in Y-394 occupancy in p56lck when associated with CD5. The use of glutathione S-transferase-Lck fusion proteins in precipitation analysis showed that the SH2 domain of p56lck could recognize CD5 as expressed in the baculovirus expression system. CD5 interaction with p56lck represents a novel variant of a receptor-kinase complex in which receptor can also serve as substrate. The CD5-p56lck interaction is likely to play roles in T-cell signalling and T-B collaboration.
Administrative Databases Provide Inaccurate Data for Surveillance of Long-Term Central Venous Catheter-Associated Infections (2003)

Claims data from different sources do not identify the same CVC insertion procedures. Current administrative databases are not ready to be used for electronic surveillance of CVC-associated complications without extensive modification and validation.
Characterization of metals emitted from motor vehicles. (2006)

A systematic approach was used to quantify the metals present in particulate matter emissions associated with on-road motor vehicles. Consistent sampling and chemical analysis techniques were used to determine the chemical composition of particulate matter less than 10 microm in aerodynamic diameter (PM10*) and particulate matter less than 2.5 microm in aerodynamic diameter (PM2.5), including analysis of trace metals by inductively coupled plasma mass spectrometry (ICP-MS). Four sources of metals were analyzed in emissions associated with motor vehicles: tailpipe emissions from gasoline- and diesel-powered vehicles, brake wear, tire wear, and resuspended road dust. Profiles for these sources were used in a chemical mass balance (CMB) model to quantify their relative contributions to the metal emissions measured in roadway tunnel tests in Milwaukee, Wisconsin. Roadway tunnel measurements were supplemented by parallel measurements of atmospheric particulate matter and associated metals at three urban locations: Milwaukee and Waukesha, Wisconsin, and Denver, Colorado. Ambient aerosol samples were collected every sixth day for one year and analyzed by the same chemical analysis techniques used for the source samples. The two Wisconsin sites were studied to assess the spatial differences, within one urban airshed, of trace metals present in atmospheric particulate matter. The measurements were evaluated to help understand source and seasonal trends in atmospheric concentrations of trace metals. ICP-MS methods have not been widely used in analyses of ambient aerosols for metals despite demonstrated advantages over traditional techniques. In a preliminary study, ICP-MS techniques were used to assess the leachability of trace metals present in atmospheric particulate matter samples and motor vehicle source samples in a synthetic lung fluid.
Using Angle of Arrival (Bearing) Information for Localization in Robot Networks (2007)

In this paper, we consider using angle of arrival information (bearing) for localization in robot networks. The essential property we require in this paper is that a node can infer heading information from its neighbors. We address the uniqueness of network localization solutions by the theory of globally rigid graphs. We show that while the parallel rigidity problem for formations with bearings is isomorphic to the distance case, the global rigidity of the formation is simpler (in fact identical to the simpler rigidity case) for a network with bearings, compared to formations with distances. We provide the conditions of localization for networks in which the neighbor relationship is not necessarily symmetric.
Open source virtual private network experience in classroom (2009)

Open VPN is an Open Source software, which is freely available for a number of different operating systems. The software is used to create a virtual private network (VPN), connecting a remote user or a fixed site to another remote network using the public Internet. Strong encryption is used to ensure that data passing back and forth over the public Internet is secure. The first part of this paper will focus on the basic concept of Open VPN software. Then the technologies and technical issues will be discussed in detail. The second part of the paper will focus towards the classroom experiment of Open VPN. This is done by using minimal hardware and available open-source software required to demonstrate Open VPN communications in action. As a result, students will learn (1) how to set up an Open VPN laboratory, (2) how to install Open VPN, (3) how to configure VPN client and server, (3) how to set up the network with the static key and dynamic key configuration files, and (4) how to test the connections stability and security in a wired and wireless environment as a home and mobile user.
The topoisomerase IIβ circular clamp arrests transcription and signals a 26S proteasome pathway (2003)

It has been proposed that the topoisomerase II (TOP2)beta-DNA covalent complex arrests transcription and triggers 26S proteasome-mediated degradation of TOP2beta. It is unclear whether the initial trigger for proteasomal degradation is due to DNA damage or transcriptional arrest. In the current study we show that the TOP2 catalytic inhibitor 4,4-(2,3-butanediyl)-bis(2,6-piperazinedione) (ICRF-193), which traps TOP2 into a circular clamp rather than the TOP2-DNA covalent complex, can also arrest transcription. Arrest of transcription, which is TOP2beta-dependent, is accompanied by proteasomal degradation of TOP2beta. Different from TOP2 poisons and other DNA-damaging agents, ICRF-193 did not induce proteasomal degradation of the large subunit of RNA polymerase II. These results suggest that proteasomal degradation of TOP2beta induced by the TOP2-DNA covalent complex or the TOP2 circular clamp is due to transcriptional arrest but not DNA damage. By contrast, degradation of the large subunit of RNA polymerase II is due to a DNA-damage signal.
Experimental study of spiral resonators for acceleration of low velocity ions (1975)

The performance of copper spiral resonators operating at'room temperature has been studied for possible applications in the acceleration of heavy ions.Many resonator models containing one, two, or three axially spaced spiral structures have been constructed and characterized at frequencies in the range 45-55,80-84, and 99-106 MHz.Resonators with a variety of drift tube diameters have shunt impedance values in the range 30 to 74 megohms per meter.Six resonators have been tested at power levels of 10-20 kilowatts, and accelerating fields up to 2.6 megavolts per meter.The 100 MHz power source used in the experiments and the feedback control for amplitude, phase, and frequency are described.Natural alpha particles were accelerated with a three-spiral resonator and their energy gain was measured as a function of their detection time relative to the radiofrequency phase.The results of these tests show that spiral resonators have improved characteristics compared to short helix resonators of the same phase velocity.
Antigenic response to topically applied proteins (1975)

Six different proteins varying widely in molecular weight, ribonuclease, lysostaphin, ovalbumin, penicillinase, collagenase, and Varidase were tested for their ability to induce circulating antibody formation in rabbits after repeated topical application of the proteins in a water-soluble gel vehicle. After a 12-week exposure period, significant hemagglutinin titers were noted in rabbits treated with ovalbumin, lysostaphin, or ribonuclease; markedly elevated, passive cutaneous anaphylaxis-reacting sera were obtained only from collagenase- or lysostaphin-treated animals. Precipitin antibodies as evidenced by gel diffusion were also found in sera from collagenas- and lysostaphin-treated animals. Topical application of penicillinase was only marginally effective and Varidase was totally ineffective in elicting a positive circulating antibody response. In all cases, topical application of proteins for periods in excess of 3 weeks was required for induction of circulating antibody formation.
Photochemical nucleation and growth of palladium on titanium dioxide films studied with electron microscopy and quantitative analytical techniques (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPhotochemical nucleation and growth of palladium on titanium dioxide films studied with electron microscopy and quantitative analytical techniquesJan W. M. JacobsCite this: J. Phys. Chem. 1986, 90, 24, 6507–6517Publication Date (Print):November 1, 1986Publication History Published online1 May 2002Published inissue 1 November 1986https://pubs.acs.org/doi/10.1021/j100282a019https://doi.org/10.1021/j100282a019research-articleACS PublicationsRequest reuse permissionsArticle Views127Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Swine diagnostic pathology (1998)

A 350 sow farrow to finish operation.Farrowing in all-in, all-out groups of 70 every 42 days.All sows have a deliberate 21-day skip heat in this schedule.The breeding and gestation is a total crated facility.The sow herd has a very high total born litter size and excellent 21-day weaning weights, 7150 lb.Nursery and grow/finish are on-site and are all-in, all-out by building.Overall health status is very good.Replacement stock is in the form of grandparent stock, and replacements are made internally.The majority of matings are A.I. with on-farm collecting and processing of semen.Replacements are purebred animals from an SPF source. Clinical signsOccur only in females that are produced from grandparent stock, primarily gilts but occasionally pI or p2's.Animals would eat but became very anxious after eating then would progress to continuous movement in the stall (front of stall to back of stall) and some self mutilation.Animals would not lay down.Body temperatures would be 105-106F.Gilts and sows would abort if not treated. Diagnosis
Renal Denervation: Current Status and Future Applications (2014)

Despite the variety of antihypertensive agents that are available, resistant hypertension remains a significant clinical problem and a substantial economic burden. Over the past several years, renal sympathetic denervation has been introduced as a potential therapeutic option for this clinical problem. It is a catheter-based procedure that is showing promising results and appears to be associated with minimal or low risk. Thus far, two completed clinical trials have demonstrated excellent safety and encouraging outcomes. A review of these trials is the focus of this article, in addition to the analysis of ongoing studies, and the possible future applications of this technique.
Cine: hacia una arqueología de las porterías. (2004)

El presente articulo esta basado en un trabajo de campo llevado a cabo en la ciudad de Barcelona en el marco de una investigacion sobre las porterias y los porteros en dicha ciudad. El trabajo tiene como proposito ilustrar, por medio del cine, la observacion participante llevada a cabo en la investigacion. Para ello, se pone el acento en los conceptos de cuerpo, liminalidad y comunidad, con el objetivo de describir cada una de las figuras de porteros/as que aparecen representadas en las peliculas y que sirven para graficar el trabajo de campo. En el mismo movimiento se sigue una linea temporal que ordena la sucesion del soporte documental, que comienza con la aparicion de la institucion a principios del siglo XX y que finaliza hacia fines de la decada del noventa.