gebhart/patrepeval huggingface dataset:
Latent Membrane Protein-1 as a Vacciine Adjuvant (2014)

of a dissertation at the University of Miami. Dissertation supervised by Professor Geoffrey Stone. No. of pages in text. (87) Dendritic cells are a promising cell type for immunotherapy due to their potent immunostimulatory potential. Nevertheless, improper maturation may account for the limited efficacy of dendritic cells in immunotherapy clinical trials. Latent Membrane Protein-1 (LMP1) of the Epstein-Barr virus was examined for its ability to mature and activate dendritic cells as a gene-based molecular adjuvant. Dendritic cells were transduced with an adenoviral vector expressing LMP1 under the control of a Tetinducible promoter. LMP1 was found to mature and activate both human and mouse dendritic cells. LMP1 enhanced in vitro migration of dendritic cells toward CCL19, as well as in vivo migration of dendritic cells to the inguinal lymph nodes of mice following subcutaneous injection. LMP1-transduced dendritic cells increased T cell proliferation in a Pmel-1 adoptive transfer model and enhanced survival in a B16-F10 melanoma tumor model. LMP1 also enhanced protection in a vaccinia-gag viral challenge model. Surprisingly, LMP1 generated high levels of IL-12p70 secretion in mouse dendritic cells and failed to induce dendritic cell exhaustion when compared to Mimic maturation. LMP1-transduced human dendritic cells were able to secrete IL-12p70 and TNF alpha in response to restimulation. In contrast, Mimic matured DC were impaired in IL-12p70 secretion following restimulation. We propose that LMP1 is a promising molecular adjuvant for dendritic cell therapy, able to mature and activate dendritic cells while allowing IL-12p70 secretion in response to restimulation in the draining lymph node.
A Novel 5-Lipoxygenase-Activating Protein Inhibitor, AM679, Reduces Inflammation in the Respiratory Syncytial Virus-Infected Mouse Eye (2009)

Respiratory syncytial virus (RSV) is an important cause of viral respiratory disease in children, and RSV bronchiolitis has been associated with the development of asthma in childhood. RSV spreads from the eye and nose to the human respiratory tract. Correlative studies of humans and direct infection studies of BALB/c mice have established the eye as a significant pathway of entry of RSV to the lung. At the same time, RSV infection of the eye produces symptoms resembling allergic conjunctivitis. Cysteinyl leukotrienes (CysLTs) are known promoters of allergy and inflammation, and the first step in their biogenesis from arachidonic acid is catalyzed by 5-lipoxygenase (5-LO) in concert with the 5-LO-activating protein (FLAP). We have recently developed a novel compound, AM679, which is a topically applied and potent inhibitor of FLAP. Here we show with the BALB/c mouse eye RSV infection model that AM679 markedly reduced the RSV-driven ocular pathology as well as the synthesis of CysLTs in the eye. In addition, AM679 decreased the production of the Th2 cell cytokine interleukin-4 but did not increase the viral load in the eye or the lung. These results suggest that FLAP inhibitors may be therapeutic for RSV-driven eye disease and possibly other inflammatory eye indications.
Dynamic spectrum management: the technology harvesting the underutilized spectrum resource [Message from the Editor-in-Chief] (2010)

Dear Readers, I hope you have endured the hot summer weather. Well, this has given you more opportunity to read our magazine! Just because you stay inside the cooling zones, this may leave much more spectrum underutilized in some outdoor areas. If the idling licensed spectra can be opportunistically utilized by others, we could enrich people's lives by using the unused spectrum resource for other good cause; for example, we might gather more data with better communications systems to resolve the recent oil spill problem in the Gulf!
Quantitative conversion of carboxylic acids and phenols to esters and ethers by reaction of their salts with alkyl halides (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative conversion of carboxylic acids and phenols to esters and ethers by reaction of their salts with alkyl halidesJames E. Shaw and Dennis C. KunerthCite this: J. Org. Chem. 1974, 39, 13, 1968–1970Publication Date (Print):June 1, 1974Publication History Published online1 May 2002Published inissue 1 June 1974https://pubs.acs.org/doi/10.1021/jo00927a048https://doi.org/10.1021/jo00927a048research-articleACS PublicationsRequest reuse permissionsArticle Views561Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Metabolomics strategy reveals therapeutical assessment of limonin on nonbacterial prostatitis (2015)

Limonin has been found to possess significant anti-inflammatory properties in animal tests and with, human cells, however, its precise metabolism mechanism has not been well explored. The aim of this study was to investigate the anti-inflammatory effects of limonin in a nonbacterial prostatitis (NBP) animal model. Global metabolite profiling was performed by ultra-high-performance liquid chromatography combined with time-of-flight mass spectrometry (UPLC/ESI-TOFMS) and in conjunction with multivariate data analysis and pathway analysis which were integrated to explore differentiating metabolites and clarify the mechanism of limonin against capsaicin-induced NBP. Limonin has a potential protective function revealed by the metabolic profiling of limonin-treated rats located closer to the normal group. Twenty potential biomarker candidates and several key metabolic pathways contributing to the treatment of NBP were discovered and identified. Among the pathways, the related glycine, serine and threonine metabolism, glycerophospholipid metabolism were acutely perturbed. The changes in metabolites were restored to their base-line levels after limonin treatment, which might be through regulating the perturbed pathways to the normal state. The results indicate that changed biomarkers and pathways may provide evidence and insight into limonin action mechanisms and enable us to increase research productivity toward metabolomics in therapeutical assessment and drug discovery.
Polymer Injection in Deep Offshore Field: The Dalia Angola Case (2008)

Abstract Whereas on-shore polymer injection may be qualified as a mature EOR technique, considering the hundreds of operations that have been conducted all over the world, only one polymer pilot has been implemented offshore, and none in deep offshore conditions. A very thorough feasibility study of polymer injection has been made on the Dalia field in Angola, a typical deep-offshore high permeability (&gt;1D as an average) sandstone reservoir containing medium viscosity oil (3 to 7cP under reservoir conditions). The study has demonstrated that high molecular weight hydrolyzed polyacrylamides could be used under a wide range of salinities covering sea water and a mixture of sea water and produced water. Additional recoveries in the range of 3 to 7 % can be expected in this particular context of large well-spacing development of a medium viscosity field. Powder polymer supply is achievable for deep offshore fields either with a specific bulk carrier or using standard international containers to transport big bags (750 kg). Although the on-deck option is simpler, even in the case where no room is left on the existing FPSO, marine options can be found to safely process the polymer on a barge connected to the FPSO (depending on the sea conditions), but the on-deck option is more simple. The need for an injectivity pilot is compulsory to demonstrate the operability of the facilities, and the injectivity of the polymer solution. A single well test has been designed and is planned fall 2008 in Luanda on one of the well of the Camelia reservoir. A skid dedicated to the injectivity test has been designed, assembled and constructed to prepare a mother solution of polymer from powder. Tested in France (no injection), the skid has been shipped to Luanda for installation on the FPSO during summer 2008. Future of the project will depend on the injectivity tests results and on-going studies on a phased approach.
Current Methods for Fluorescence-Based Universal Sequence-Dependent Detection of Nucleic Acids in Homogenous Assays and Clinical Applications (2013)

The current methods for USD NA testing are cost-effective and flexible and have concordant analytical performance in comparison with common probe-based techniques. They can detect any target sequence by the simple use of a label-free, low-cost primer or probe combined with a universal fluorogenic reporter. The methods differ in the number of target specificities, capability of multiplexing, and incubation requirements (isothermal/thermocycling). Extensive clinical applications comprise detection of single-nucleotide polymorphisms, study of gene expression, in situ PCR, and quantification of pathogen load.
Hydrogen localization near boron in silicon (1985)

Several models of boron neutralized by atomic hydrogen in silicon were tested by secondary ion mass spectrometry and infrared spectrometry. The hydrogen concentration is comparable to that of boron. Boron neutralization appears as a drop in free-carrier absorption and as an increase in resistivity. A new infrared vibrational mode attributed to 〈111〉 vibrations of H tied to Si appears at 1875 cm−1.
Droplet microfluidics for amplification-free genetic detection of single cells (2012)

In this article we present a novel droplet microfluidic chip enabling amplification-free detection of single pathogenic cells. The device streamlines multiple functionalities to carry out sample digitization, cell lysis, probe-target hybridization for subsequent fluorescent detection. A peptide nucleic acid fluorescence resonance energy transfer probe (PNA beacon) is used to detect 16S rRNA present in pathogenic cells. Initially the sensitivity and quantification abilities of the platform are tested using a synthetic target mimicking the actual expression level of 16S rRNA in single cells. The capability of the device to perform "sample-to-answer" pathogen detection of single cells is demonstrated using E. coli as a model pathogen.
Uptake and metabolism of polyprenols by animal cells cultured in vitro (1981)

Several mammalian, chicken, and mosquito cells grown in vitro take up tritiated dolichol supplied to the incubation medium. The extent of labelling varied markedly between different cell cultures. After 20 h incubation most of the dolichol taken up was unchanged and the major product of metabolism of dolichol was identified as its fatty acid esters. Green-monkey kidney cells were tested with 8 fully unsaturated and 6 alpha-saturated polyprenols ranging from C35 to C105. In general the uptake of alpha-saturated polyprenols (dolichol type, was higher. Considerable differences were found between the uptake of polyprenols of differing chain lengths. Less than 1% of the polyprenols taken up was converted into more polar product, mainly polyprenyl phosphates and polyprenyl phosphate sugars. The short-chain polyprenols, from C35 to C65, were metabolized more rapidly than the long-chain polyprenols, as judged from the amount of polar products and fatty acid esters of polyprenols.
Under-diagnosed psychiatric syndrome. II: Pathologic skin picking. (1999)

Pathologic skin picking (PSP), previously known as neurotic excoriation, is characterised by habitual and repetitive self-excoriation of skin. Affecting mainly women with onset in the 20s and 30s, it can lead to significant distress, dysfunction and disfigurement. The extent and degree of self-excoriation is reported to be proportional to the distortion of the underlying personality. Because of the close similarity to obsessive compulsive disorder in phenomenology and pharmacological response to the serotonin re-uptake inhibitor (e.g. clomipramine, fluoxetine and sertraline), PSP has been considered as an obsessive compulsive spectrum disorder.
‘Fingerprints’ of Central Stimulatory Drug Effects by Means of Quantitative Radioelectroencephalography in the Rat (Tele-Stereo-EEG) (1986)

The new electrophysiological model earlier described as stereo-EEG is extended now to allow recording from the freely moving rat by means of a telemetric device. Chronic implantation of 4 electrodes into the brain allows simultaneous transmission of field potentials from frontal cortex, hippocampus, striatum and reticular formation. Frequency analysis of these potentials results in a drug-specific 'fingerprint' which cannot only be used to compare different chemicals with each other but also to detect onset and time dependence of drug actions. Application of the model to the question if fenetylline has its own intrinsic mode of action or merely develops its stimulatory effect after metabolic separation into its molecular moieties amphetamine and theophylline (prodrug hypothesis) revealed that fenetylline indeed displays its own stimulatory effect to the same extent and at a similar time course as amphetamine and theophylline. The 'fingerprint' as obtained by the analysis of the action of fenetylline in the rat resembles closely that obtained after the application of theophylline with respect to decreased alpha activity, but resembles amphetamine with respect to beta 1 activity. Thus the applied method allows studying structure function relationships as the action of fenetylline seems to reflect both its molecular moieties.
Online Antenna Tilt-Based Capacity and Coverage Optimization (2014)

In the field of self-organizing networks (SONs), the use case of concurrent capacity and coverage optimization (CCO) is challenging since the objectives are typically conflicting and adequate solutions often require interaction between a multitude of network sites. As a consequence, solving the use case of CCO is particularly difficult. This letter proposes an efficacious modification of the antenna tilt-based SON algorithm by Berger et al. We propose using a probability distribution function (PDF) of throughput measurements and an estimate of the number of covered and uncovered users of each cell considered for optimization in order to construct an objective function that is better suited to jointly maximize throughput and coverage. Due to the limited amount of data required for computing the algorithm's objective function, the SON solution proposed can be implemented not only following a centralized but also following a distributed architecture. Simulations show that the SON algorithm can exploit this additional information to increase its performance in terms of throughput gains.
Mucositis (2015)

Oral mucositis is a common complication of cancer treatment that may negatively impact the patient's cancer treatment outcome. Despite its frequency and consequences, the lack of effective interventions has frustrated patients and caregivers. Fortunately, a broad range of mechanistically targeted compounds are being developed.
Retina and Optic Nerve (1962)

Our website uses cookies to enhance your experience. By continuing to use our site, or clicking "Continue," you are agreeing to our Cookie Policy | Continue JAMA Ophthalmology HomeNew OnlineCurrent IssueFor Authors Podcast Publications JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry (1919-1959) JN Learning / CMESubscribeJobsInstitutions / LibrariansReprints & Permissions Terms of Use | Privacy Policy | Accessibility Statement 2023 American Medical Association. All Rights Reserved Search All JAMA JAMA Network Open JAMA Cardiology JAMA Dermatology JAMA Forum Archive JAMA Health Forum JAMA Internal Medicine JAMA Neurology JAMA Oncology JAMA Ophthalmology JAMA Otolaryngology–Head & Neck Surgery JAMA Pediatrics JAMA Psychiatry JAMA Surgery Archives of Neurology & Psychiatry Input Search Term Sign In Individual Sign In Sign inCreate an Account Access through your institution Sign In Purchase Options: Buy this article Rent this article Subscribe to the JAMA Ophthalmology journal
Immunoreactive forms of caldesmon in cultivated human vascular smooth muscle cells (1987)

150 kDa caldesmon was shown to be characteristic of vascular smooth muscle cells in normal tissue rather than in subculture. Subcultured smooth muscle cells from human aorta contained only the 70 kDa immunoreactive form of caldesmon. During the course of primary culture the amount of 150 kDa caldesmon as well as metavinculin decreased significantly whilst 70 kDa caldesmon became the predominant form, and by the onset of cell division the 150 kDa form was practically substituted by 70 kDa caldesmon. The data show that the predominance of 150 kDa caldesmon is characteristic of contractile smooth muscle cells, while in proliferating cells 70 kDa caldesmon is expressed.
Normalization of oligonucleotide arrays based on the least-variant set of genes (2008)

We show that LVS normalization outperforms other normalization methods when the standard assumptions are not satisfied. In the complex spike-in study, LVS performs similarly to the ideal (in practice unknown) housekeeping-gene normalization. An R package called lvs is available in http://www.meb.ki.se/~yudpaw.
Neutrophil Proteases Can Inactivate Human PAR3 and Abolish the Co-receptor Function of PAR3 on Murine Platelets (2001)

Three members of the protease-activated receptor family, PAR1, PAR3 and PAR4, are activated when thrombin cleaves the receptor N-terminus, exposing a tethered ligand. Proteases other than thrombin can also cleave PAR family members and, depending upon whether this exposes or removes the tethered ligand, either activate or disable the receptor. For example, on human platelets PAR1 is disabled by cathepsin G, although aggregation still occurs because cathepsin G can activate PAR4. The present studies examine the interaction of cathepsin G and a second neutrophil protease, elastase, with PAR3 using two model systems: COS-7 cells transfected with human PAR3 and mouse platelets, which express PAR3 and PAR4, but not PAR1. In contrast to human platelets, cathepsin G did not aggregate murine platelets, and prevented their activation only at low thrombin concentrations. Elastase had no effect on thrombin responses in mouse platelets, but when added to COS cells expressing human PAR3, both cathepsin G and elastase prevented activation of phospholipase C by thrombin. Notably, this inhibition occurred without loss of the binding sites for two monoclonal antibodies that flank the tethered ligand on human PAR3. We therefore conclude that 1) exposure to cathepsin G disables signaling through human PAR3, and prevents murine PAR3 from serving its normal role, which is to facilitate PAR4 cleavage at low thrombin concentrations, 2) elastase disables human, but not murine, PAR3, 3) in contrast to human PAR4, mouse PAR4 will not support platelet aggregation in response to cathepsin G, and 4) the inactivation of human PAR3 by cathepsin G and elastase involves a mechanism other than amputation of the tethered ligand domain. These results extend the range of possible interactions between PAR family members and proteases, and provide further support for species-specific differences in the interaction of these receptors with proteases other than thrombin.
Synthesis and anticonvulsant and sedative-hypnotic activity of 4-(alkylimino)-2,3-dihydro-4H-1-benzopyrans and benzothiopyrans (1990)

A series of 4-(alkylimino)-5-hydroxy-7-alkyl-2,3-dihydro-4H-1-benzopyrans and -thiopyrans were synthesized and evaluated for anticonvulsant activity. Preliminary screening of these compounds revealed that 2,2-dimethyl-4-[(2-hydroxyalkyl)imino]-5-hydroxy-7-pentyl-2,3- dihydro-4H-1-benzopyrans 19 and 29, the 7-butyl analogue 34, and the corresponding 7-pentyl-4H-1-benzothiopyrans 38 and 39 had the most promising anticonvulsant activity. Synthesis of both enantiomers of 29 and 39 indicated that the R isomers 30 and 40 were the most active and showed very good protection against MES, pentylenetetrazole, and mercaptopropionic acid induced seizures after oral administration in mice. In the Irwin test these compounds showed a generalized depressant activity but at dosages higher than those showing anticonvulsant activity, whereas acute toxicity after oral administration was low (LD50 higher than 400 mg/kg).
Copper-mediated C–H(sp<sup>2</sup>)/C–H(sp<sup>3</sup>) coupling of benzoic acid derivatives with ethyl cyanoacetate: an expedient route to an isoquinolinone scaffold (2014)

A facile, copper-mediated, direct C-H(sp(2))/C-H(sp(3)) bond coupling of benzoic acid derivatives with ethyl cyanoacetate by the deployment of an 8-aminoquinoline moiety as a bidentate directing group is disclosed. Such a unique transformation provides a new strategy for the construction of an isoquinolinone scaffold as one of the privileged cores.
Effect of Scale Dependent Data Correlations in an Integrated History Matching Loop Combining Production Data and 4D Seismic Data (2003)

Abstract The use of time-lapse data, or 4D seismic, in conjunction with production data in computer aided history matching of reservoir models requires that the various types of data are incorporated in a single objective function measuring the mismatch between the simulated and measured data. In the context of linear maximum likelihood estimation, the contribution of seismic data and production data to the objective function can be balanced on the basis of the sum of the inverse of the data and model error covariance matrices. Methods to estimate these covariance matrices for the combined set of production data and seismic impedance are presented. Generally, the seismic data will be correlated leading to a nondiagonal error covariance matrix. This matrix will also be very large, and efficient methods to invert this matrix are required. This is done using a very fast discrete convolution inverse based on multiplication of block Toeplitz matrices. It is shown that the regression may converge to a wrong solution if incorrect values for the data correlations are used. The presented methodology is applied to an actual history-matching project using data from a North Sea oil field, and a procedure for mapping the time-lapse seismic data and covariance matrix from the seismic grid to the simulation grid is presented. The data seems to contain information about distribution of gas and oil not recovered by history matching to production data only. However, it turned out to be difficult to obtain a good match to the time-lapse seismic data in the regression. It is not clear whether this is due to large uncertainties in the data, incorrect petroelastic model, or incorrect parameterization. This should be investigated in future work.