gebhart/patrepeval huggingface dataset:
Towards high-throughput automated targeted femtosecond laser-based transfection of adherent cells (2011)

Femtosecond laser induced cell membrane poration has proven to be an attractive alternative to the classical methods of drug and gene delivery. It is a selective, sterile, non-contact technique that offers a highly localized operation, low toxicity and consistent performance. However, its broader application still requires the development of robust, high-throughput and user-friendly systems. We present a system capable of unassisted enhanced targeted optoinjection and phototransfection of adherent mammalian cells with a femtosecond laser. We demonstrate the advantages of a dynamic diffractive optical element, namely a spatial light modulator (SLM) for precise three dimensional positioning of the beam. It enables the implementation of a "point-and-shoot" system in which using the software interface a user simply points at the cell and a predefined sequence of precisely positioned doses can be applied. We show that irradiation in three axial positions alleviates the problem of exact beam positioning on the cell membrane and doubles the number of viably optoinjected cells when compared with a single dose. The presented system enables untargeted raster scan irradiation which provides transfection of adherent cells at the throughput of 1 cell per second.
The Electrodeposition of Platinum and Platinum Alloys (1988)

Work on the electrolytic deposition of platinum and some platinum alloys is reviewed, and the results of our recent work on the deposition and the properties of the layers produced from some promising electrolytes are briefly discussed. In general, studies of plating solutions are restricted by the economic availability of the relevant platinum salts. On the other hand the hydrolysis of the platinum salts in solution, and the incorporation of decomposition products are also critical factors, especially for their influence on internal stress. Recent work has shown that it is possible to deposit platinum-cobalt alloys which have excellent magnetic and mechanical properties, and these alloy deposits look very promising for data storage applications and for small permanent magnet layers.
Protection from HIV-1 Infection of Primary Cd4 T Cells by Ccr5 Silencing is Effective for the Full Spectrum of Ccr5 Expression (2003)

Stable gene silencing by RNA interference (RNAi) can be achieved by expression of small hairpin RNAs (shRNAs) from RNA polymerase III promoters. We have tested lentiviral vectors expressing shRNAs targetting CCR5 in primary CD4 T cells from donors representing various CCR5 and CCR2 genetic backgrounds covering the full spectrum of CCR5 expression levels and permissiveness for HIV-1 infection. A linear decrease in CCR5 expression resulted in a logarithmic decrease in cellular infection, giving up to three logs protection from HIV-1 infection in vitro. Protection was maintained at very high multiplicity of infection. This and other recent reports on RNAi should open a debate about the use of RNAi gene therapy for HIV infection.
Automated bone density calculation using feature extraction by deformable templates (2002)

The authors propose a method for detecting and describing features in medical images by using deformable templates for the purpose of diagnostic analysis of these features. The feature of interest can be described by a parameterized template. A gradient maximization technique is used to change the parameters so that the template fits onto the desired anatomical feature. The final parameter values can be used as descriptors for the feature. Measurements of intensity values within a region of the template can be used as inputs to a medical diagnostic system. A picture-archive-and-communication-system-based image analysis program, which uses the technique of deformable templates to localize features in dual energy computer tomography (CT) images, is discussed. Statistical comparisons between the system and previously used manual techniques show that the performances are practically equivalent and that the system has several advantages over the human operator, for example, consistency, accuracy, and cost.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Read performance enhancement in data deduplication for secondary storage (2013)

University of Minnesota M.S. thesis. May 2013. Major: Computer science. Advisor: David Hung-Chang Du. 1 computer file (PDF); vii, 41 pages.
The Rule in Shelley's Case in Pennsylvania (1915)

The Rule in Shelley's Case is one of the most ancient and renowned in the law of real property.Although the earliest instance of its application is to be found in the year 1326,1 and several instances occur during the reign of Edward III, and also during the succeeding centuries, yet it was not until the twenty-third year of the reign of Elizabeth (i579) that the case arose which gave the rule its familiar appellation. 2As Mr.Hargrave, who was one of the editors of Coke-Littleton, tells us, it was due to the circumstance that the report of Shelley's Case by Lord Coke, who argued the case for the defendant, con-' tains the best known depository of the rule and also the fullest and most correct expression of the doctrine meant to be conveyed, that, for the sake of shortness, lawyers and judges have in all succeeding times spoken of it as the Rule in Shelley's Case.3The rule, as reported by Coke, is as follows:-"That when the ancestor by any gift or conveyance takes an estate of freehold, and in the same gift or conveyance an estate is limited either mediately or immediately to his heirs in fee, or in tail, always in such cases heirs are words of limitation of the estate, and not words of purchase."Just before the outbreak of the American Revolution, the case of Perrin v. Blake arose, in which Lord Chief Justice Mansfield, supported by two of his colleagues, Aston, and Willes, held that the intention of a testator, if clearly expressed, could control the rule, while Justice YAtes, the sole dissentient, held that a rule of law could not be destroyed by a testator.In the Exchequer Chamber the judgment of the King's Bench was reversed, and it is interesting to note that the opinion of Blackstone, sitting as a member of the Appellate Court, was opposed 'Year Book, i8th Edw.II, fol.577, Sergeant Maynard's Edition. 2 Shelley's Case, i Coke's Rep. 94-io4-'Hargrave: Jurisconsult Exercitations, Vol.III, p. 317.
Recovery protocol using a common log (1982)

The authors describe a method for minimizing synchronous writing to a shared log among concurrent referencing nodes (tasks) while preserving independent of node (task) leading order of the log.
Socially inspired data dissemination for vehicular ad hoc networks (2014)

People have routines and their mobility patterns vary during the day, which have a direct impact on vehicular mobility. Therefore, proto- cols and applications designed for Vehicular Ad Hoc Networks need to adapt to these routines in order to provide better services. With this issue in mind, in this work, we propose a data dissemination solution for these networks that considers the daily road traffic variation of large cities and the relationship among vehicles. The focus of our approach is to select the best vehicles to rebroadcast data messages according to social metrics, in particular, the clustering coefficient and the node degree. Moreover, our solution is designed in such a way that it is completely independent of the perceived road traffic density. Simulation results show that, when compared to related protocols, our proposal provides better delivery guarantees, reduces the network overhead and possesses an acceptable delay.
<i>Cecropia pachystachya</i>: A Species with Expressive<i>In Vivo</i>Topical Anti-Inflammatory and<i>In Vitro</i>Antioxidant Effects (2014)

Cecropia pachystachya is a species traditionally used in Brazil to treat inflammation. This work aims to evaluate the topical anti-inflammatory and antioxidant activities of the methanolic extract of C. pachystachya (CPM) and to perform its chemical fingerprint by HPLC-DAD. The topical anti-inflammatory activity was evaluated using the mouse models of acute ear inflammation induced by croton oil, arachidonic acid, capsaicin, EPP, phenol, and chronic inflammation induced by multiple application of croton oil. The in vitro antioxidant effect of CPM was investigated using DPPH, reducing power, β -carotene bleaching, and TBARS assays. HPLC analysis was performed to quantify the antioxidant phenolics orientin, isoorientin, and chlorogenic acid previously identified in CPM. CPM exhibited significant anti-inflammatory effect in the acute models, in some cases comparable to the reference drugs. Histopathological analysis showed a moderate chronic skin anti-inflammatory effect with decrease in vasodilation, edema, cell infiltration, and epidermal hyperproliferation. It also showed strong in vitro antioxidant activity. The contents of orientin, isoorientin, and chlorogenic acid were 66.5 ± 1.8, 118.8 ± 0.7, and 5.4 ± 0.2 µg/mg extract, respectively. The topical anti-inflammatory activity of CPM could be based on its antioxidant properties, although other effects are probably involved, including COX inhibition and other mechanisms.
Production of ML-236B, and Inhibitor of 3-Hydroxy-3-methylglutaryl CoA Reductase, by<i>Penicillium citrinum:</i>Improvements of Strain and Culture Conditions (1993)

In order to increase the yield of ML-236B, an intermediate for pravastatin Na (an inhibitor of cholesterol synthesis) production, the improvements of an ML-236B producing strain of Penicillium citrinum, the medium composition, and the culture conditions were studied. A mutant strain S-5808, which produces ML-236B 20 times more than the original strain does in flask culture, was isolated. As the cells of S-5808 required a large amount of the carbon source substrate to produce a lot of ML-236B, a fed-batch culture method was developed. The continuous feeding of carbon source (glycerol or mixture of glycerol and maltose) was very effective for the increase of ML-236B production. ML-236B in the culture broth became oily with lowering of pH during cultivation, adhered to the cell surface, decreased the carbon source consumption and finally stopped the ML-236B production at a late period of fermentation. The addition of a polypropylene glycol type surfactant enhanced the ML-236B productivity. The addition of the surfactant released oily ML-236B from the cell surface, therefore oxygen uptake, carbon source consumption, and ML-236B production continued throughout the fermentation.
Wave field reconstruction from multiple plane intensity-only data: augmented Lagrangian algorithm (2011)

A complex-valued wave field is reconstructed from intensity-only measurements given at multiple observation planes parallel to the object plane. The phase-retrieval algorithm is obtained from the constrained maximum likelihood approach provided that the additive noise is gaussian. The forward propagation from the object plane to the measurement plane is treated as a constraint in the proposed variational setting of reconstruction. The developed iterative algorithm is based on an augmented lagrangian technique. An advanced performance of the algorithm is demonstrated by numerical simulations.
Palmitoylation at Cys <sup>574</sup> is essential for MT1‐MMP to promote cell migration (2005)

MT1-MMP is a type I transmembrane proteinase that promotes cell migration and invasion. Here, we report that MT1-MMP is palmitoylated at Cys574 in the cytoplasmic domain, and this lipid modification is critical for its promotion of cell migration and clathrin-mediated internalization. The palmitoylation-defective mutant (C574A) failed to promote cell migration and was not internalized through clathrin pathway like wild-type, but it was internalized through the caveolae pathway. Reintroducing a cysteine at different positions in the cytoplasmic tail of the C574A mutant revealed that the position of the palmitoylated cysteine relative to LLY573, a motif that interacts with mu2 subunit of adaptor protein 2, is critical for the cell motility-promoting activity of MT1-MMP and its clathrin-mediated internalization. Taken together, palmitoylation of MT1-MMP is one of the key posttranslational modifications that determines MT1-MMP-dependent cell migration.
Adaptive Planning and Prediction in Agent-Supported Distributed Collaboration. (2004)

Agents that act as user assistants will become invaluable as the number of information sources continue to proliferate. Such agents can support the work of users by learning to automate time-consuming tasks and filter information to manageable levels. Although considerable advances have been made in this area, it remains a fertile area for further development. One application of agents under careful scrutiny is the automated negotiation of conflicts between different user's needs and desires. Many techniques require explicit user models in order to function. This dissertation explores a technique for dynamically constructing user models and the impact of using them to anticipate the need for negotiation. Negotiation is reduced by including an advising aspect to the agent that can use this anticipation of conflict to adjust user behavior.
PVDF-Based Nanocomposite Solid Polymer Electrolytes; the Effect of Affinity Between PVDF and Filler on Ionic Conductivity (2009)

Nanocomposite solid polymer electrolytes (NSPEs) based on poly(vinylidene fluoride) (PVDF) were prepared by dispersing two kinds of organoclay (Cloisite® 30B, Cloisite® 15A) consisting of silicate layers in the polymer matrix. The effect of affinity between PVDF and organoclay as the filler on ionic conductivity was investigated in relation to its content, dispersed condition of organoclay, and structural changes of nanocomposites. The characterizations of PVDF-based nanocomposites with various organoclay contents were carried out by XRD, TEM, DSC, and DMA. In order to confirm the ion conduction properties of NSPEs with LiCF3SO3 at room temperature, ac impedance analyzer and FT-IR spectrometer were used. As a result, a higher ionic conductivity appeared in the case of NSPE with C15A than that with C30B and the maximum conductivity was 1.04 × 10–3 S/cm for the NSPE containing 5 wt% of C15A and 40 wt% of LiCF3SO3.
Threshold-based dynamic replication in large-scale video-on-demand systems (2002)

Advances in high speed networking technologies and video compression techniques have made video-on-demand (VOD) services feasible. A large-scale VOD system imposes a large demand on bandwidth and storage resources, and therefore, parallel disks are typically used for providing VOD service. Although striping of movie data across a large number of disks can balance the utilization among these disks, such a striping technique can exhibit additional complexity, for instance, in data management, such as synchronization among disks during data delivery, as well as in supporting fault tolerant behavior. Therefore, it is more practical to limit the extent of data striping, for example, by arranging the disks in groups (or nodes) and then allowing intra-group (or intra-node) data striping only. With multiple striping groups, however, we may need to assign a movie to multiple nodes so as to satisfy the total demand of requests for that movie. Such an approach gives rise to several design issues, including: what is the right number of copies of each movie we need so as to satisfy the demand and at the same time not waste storage capacity; how to assign these movies to different nodes in the system; and what are efficient approaches to altering the number of copies of each movie (and their placement) when the need for that arises. We study an approach to dynamically reconfiguring the VOD system so as to alter the number of copies of each movie maintained on the server as the access demand for these movies fluctuates. We propose various approaches to addressing the above stated issues, which result in a VOD design that is adaptive to the changes in data access patterns. Performance evaluation is carried out to quantify the costs and the performance gains of these techniques.
Viewable scene modeling for geospatial video search (2008)

Video sensors are becoming ubiquitous and the volume of captured video material is very large. Therefore, tools for searching video databases are indispensable. Current techniques that extract features purely based on the visual signals of a video are struggling to achieve good results. By considering video related meta-information, more relevant and precisely delimited search results can be obtained. In this study we propose a novel approach for querying videos based on the notion that the geographical location of the captured scene in addition to the location of a camera can provide valuable information and may be used as a search criterion in many applications. This study provides an estimation model of the viewable area of a scene for indexing and searching and reports on a prototype implementation. Among our objectives is to stimulate a discussion of these topics in the research community as information fusion of different georeferenced data sources is becoming increasingly important. Initial results illustrate the feasibility of the proposed approach.
Prevention of postoperative capsular bag opacification using intraocular lenses and endocapsular devices maintaining an open or expanded capsular bag (2016)

None of the authors has a financial or proprietary interest in any material or method mentioned.
A Novel Intravenous Iron Formulation for Treatment of Anemia in Inflammatory Bowel Disease: The Ferric Carboxymaltose (FERINJECT<sup>®</sup>) Randomized Controlled Trial (2008)

FeCarb is effective and safe in IBD-associated anemia. It is noninferior to FeSulf in terms of Hb change over 12 wk, and provides a fast Hb increase and a sufficient refill of iron stores.
Pigment Epithelium-Derived Factor Enhances Differentiation and Mineral Deposition of Human Mesenchymal Stem Cells (2013)

Pigment epithelium-derived factor (PEDF) is a potent antiangiogenic factor found in a wide variety of tissues. Recent findings indicated that lack of PEDF leads to osteogenesis imperfecta type VI whose hallmark is a defect in mineralization. We investigated the effects of PEDF on human mesenchymal stem cells (hMSCs) and signaling pathways through which PEDF displays its activities in hMSCs. hMSCs incubated in a medium supplemented with PEDF induced expression of osteoblastic-related genes. In addition, PEDF induced alkaline phosphatase (ALP) activity in MSCs at 14 days of incubation in maintenance medium; hMSCs incubated in osteogenic medium in presence of PEDF expressed 19% more ALP activity (35.655 ± 1.827 U/mg protein, p = .041 than cells incubated in the same medium without PEDF supplementation (29.956 ± 2.100 U/μg protein). hMSCs incubated in osteogenic medium in presence of PEDF deposited 50% more mineral (2.108 ± 0.306 OD/ml per well per 1 × 10(4) cells per square centimeter, p = .017) than MSCs incubated in absence of the protein (1.398 ± 0.098 OD/ml per well per 1 × 10(4) cells per square centimeter) as determined by Alizarin Red quantitation. Reduction in PEDF expression in MSCs by siRNA led to decreased ALP activity (33.552 ± 2.009 U/ng protein of knockdown group vs. 39.269 ± 3.533 U/ng protein of scrambled siRNA group, p = .039) and significant reduction in mineral deposition (0.654 ± 0.050 OD/ml per well per 1 × 10(4) cells per square centimeter of knockdown group vs. 1.152 ± 0.132 OD/ml per well per 1 × 10(4) cells per square centimeter of wild-type group, p = .010). Decreased ALP activity and mineral deposition were restored by supplementation with exogenous PEDF protein. PEDF activated ERK and AKT signaling pathways in MSCs to induce expression of osteoblastic-related genes. These data suggest that PEDF is involved in MSCs osteoblastic differentiation.
Do Stemmed Tibial Components in Total Knee Arthroplasty Improve Outcomes in Patients With Obesity? (2016)

Level I, therapeutic study.
Method for the determination of effective loss impairment factor when sending short messages over the internet (2011)

In this paper we present the method for the determination of Effective Loss Impairment Factor in the case of sending short voice messages over the Internet. The method can be used for random packet loss and for burst packet loss. In the paper, the method is illustrated for the group packet loss. The method provides a possibility to determine the distribution of quality of different short-term voice connections.
Based on ZigBee wireless sensor network the monitoring system design for chemical production process toxic and harmful gas (2010)

Aiming at monitoring information of HCHO, CH4, LPG and other toxic and harmful gas concentrations in the chemical production plant, the monitoring system designed a ZigBee wireless sensor network composed of CC2430, MC114 and MS1100 gas sensor, which built a safe, low power consumption, flexible detection system of toxic and harmful gas concentrations. As the system data transmission with the mode of sensor test data in a short distance, multi-node, self-organizing wireless network, it could use the USB interface gateway to connect the WSN and internet to implement the data transmission with internet.
EM Localization and Separation using Interaural Level and Phase Cues (2007)

We describe a system for localizing and separating multiple sound sources from a reverberant two-channel recording. It consists of a probabilistic model of interaural level and phase differences and an EM algorithm for finding the maximum likelihood parameters of this model. By assigning points in the interaural spectrogram probabilistically to sources with the best-fitting parameters and then estimating the parameters of the sources from the points assigned to them, the system is able to separate and localize more sound sources than there are available channels. It is also able to estimate frequency-dependent level differences of sources in a mixture that correspond well to those measured in isolation. In experiments in simulated anechoic and reverberant environments, the proposed system improved the signal-to-noise ratio of target sources by 2.7 and 3.4dB more than two comparable algorithms on average.
Superhydrophobic TiO<sub>2</sub>–Polymer Nanocomposite Surface with UV-Induced Reversible Wettability and Self-Cleaning Properties (2013)

Multifunctional superhydrophobic nanocomposite surfaces based on photocatalytic materials, such as fluorosilane modified TiO2, have generated significant research interest. However, there are two challenges to forming such multifunctional surfaces with stable superhydrophobic properties: the photocatalytic oxidation of the hydrophobic functional groups, which leads to the permanent loss of superhydrophobicity, as well as the photoinduced reversible hydrolysis of the catalytic particle surface. Herein, we report a simple and inexpensive template lamination method to fabricate multifunctional TiO2-high-density polyethylene (HDPE) nanocomposite surfaces exhibiting superhydrophobicity, UV-induced reversible wettability, and self-cleaning properties. The laminated surface possesses a hierarchical roughness spanning the micro- to nanoscale range. This was achieved by using a wire mesh template to emboss the HDPE surface creating an array of polymeric posts while partially embedding untreated TiO2 nanoparticles selectively into the top surface of these features. The surface exhibits excellent superhydrophobic properties immediately after lamination without any chemical surface modification to the TiO2 nanoparticles. Exposure to UV light causes the surface to become hydrophilic. This change in wettability can be reversed by heating the surface to restore superhydrophobicity. The effect of TiO2 nanoparticle surface coverage and chemical composition on the mechanism and magnitude of wettability changes was studied by EDX and XPS. In addition, the ability of the surface to shed impacting water droplets as well as the ability of such droplets to clean away particulate contaminants was demonstrated.