gebhart/patrepeval huggingface dataset:
Conserved valproic-acid-induced lipid droplet formation in <i>Dictyostelium</i> and human hepatocytes identifies structurally active compounds (2011)

Lipid droplet formation and subsequent steatosis (the abnormal retention of lipids within a cell) has been reported to contribute to hepatotoxicity and is an adverse effect of many pharmacological agents including the antiepileptic drug valproic acid (VPA). In this study, we have developed a simple model system (Dictyostelium discoideum) to investigate the effects of VPA and related compounds in lipid droplet formation. In mammalian hepatocytes, VPA increases lipid droplet accumulation over a 24-hour period, giving rise to liver cell damage, and we show a similar effect in Dictyostelium following 30 minutes of VPA treatment. Using (3)H-labelled polyunsaturated (arachidonic) or saturated (palmitic) fatty acids, we shown that VPA treatment of Dictyostelium gives rise to an increased accumulation of both types of fatty acids in phosphatidylcholine, phosphatidylethanolamine and non-polar lipids in this time period, with a similar trend observed in human hepatocytes (Huh7 cells) labelled with [(3)H]arachidonic acid. In addition, pharmacological inhibition of β-oxidation in Dictyostelium phenocopies fatty acid accumulation, in agreement with data reported in mammalian systems. Using Dictyostelium, we then screened a range of VPA-related compounds to identify those with high and low lipid-accumulation potential, and validated these activities for effects on lipid droplet formation by using human hepatocytes. Structure-activity relationships for these VPA-related compounds suggest that lipid accumulation is independent of VPA-catalysed teratogenicity and inositol depletion. These results suggest that Dictyostelium could provide both a novel model system for the analysis of lipid droplet formation in human hepatocytes and a rapid method for identifying VPA-related compounds that show liver toxicology.
A spreadsheet method for studying neural networks (2002)

A unified framework and method for studying small neural networks (up to 75 neurons) using a computer spreadsheet is described. Neural networks actually resemble spreadsheets in several ways. A neural network consists of many simple computational units, highly interconnected and operating in parallel. Each unit has a numerical value (an output), which it communicates to other units along connections of varying strength. Similarly, a spreadsheet contains several thousand cells, arranged in rows and columns, appearing to perform in parallel. The numerical values of certain cells (i.e. their outputs) become parameters for calculating the values of others linked to them via suitable formulas. Just as the units of most networks are identical to each other, the formulas of spreadsheet cells are often highly repetitive, except for the relative location of cells which they reference. The authors do not advocate that all artificial neural networks be implemented on a spreadsheet. However, the spreadsheet is a valuable research tool and learning aid.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Comparison of Two Major Emergency Department-Based Free-Text Chief-Complaint Coding Systems (2004)

Although this analysis revealed optimal overall agreement between the two systems evaluated, substantial differences in classification schemes existed, highlighting the need for a consensus regarding chief-complaint classification.
Multilevel asymmetric power converters for switched reluctance machines (2002)

This paper presents a new family of multilevel asymmetric power converters which are suitable for unipolar current loads such as a switched reluctance motor drive. The fundamental operation of each topology is reviewed and some simulation results are presented showing the potential system performance improvements that can be realised by operating with intermediary voltages rather than the full DC-link.
Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children (2006)

Conventional fluorescent-antibody (FA) methods were compared to real-time PCR assays for detection of respiratory syncytial virus (RSV), influenza virus type A (FluA), parainfluenza virus types 1, 2, and 3 (PIV1, PIV2, and PIV3), human metapneumovirus (MPV), and adenovirus (AdV) in 1,138 specimens from children with respiratory illnesses collected over a 1-year period. At least one virus was detected in 436 (38.3%) specimens by FA and in 608 (53.4%) specimens by PCR (P<0.001). Specimen quality was inadequate for FA in 52 (4.6%) specimens; 13 of these (25%) were positive by PCR. In contrast, 18 (1.6%) specimens could not be analyzed by PCR; 1 of these was positive by FA. The number of specimens positive only by PCR among specimens positive by PCR and/or FA was 18 (7.0%) of 257 for RSV, 18 (13.4%) of 134 for FluA, 25 (64.1%) of 39 for PIV1, 8 (88.9%) of 9 for PIV2, 17 (30.1%) of 55 for PIV3, and 101 (76.5%) of 132 for AdV. MPV was detected in 6.6% of all specimens and in 9.5% of the 702 specimens negative by FA. The mean number of virus copies per milliliter in specimens positive by both PCR and FA was significantly higher, at 6.7x10(7), than that in specimens positive only by PCR, at 4.1x10(4) (P<0.001). The PCR assays were significantly more sensitive than FA assays for detecting respiratory viruses, especially parainfluenza virus and adenovirus. Use of real-time PCR to identify viral respiratory pathogens in children will lead to improved diagnosis of respiratory illness.
A novel prognostic model in elderly patients with acute myeloid leukemia: results of 909 patients entered into the prospective AML96 trial (2010)

We present an analysis of prognostic factors derived from a trial in patients with acute myeloid leukemia older than 60 years. The AML96 trial included 909 patients with a median age of 67 years (range, 61-87 years). Treatment included cytarabine-based induction therapy followed by 1 consolidation. The median follow-up time for all patients is 68 months (5.7 years). A total of 454 of all 909 patients reached a complete remission (50%). Five-year overall survival (OS) and disease-free survival were 9.7% and 14%, respectively. Multivariate analyses revealed that karyotype, age, NPM1 mutation status, white blood cell count, lactate dehydrogenase, and CD34 expression were of independent prognostic significance for OS. On the basis of the multivariate Cox model, an additive risk score was developed that allowed the subdivision of the largest group of patients with an intermediate-risk karyotype into 2 groups. We are, therefore, able to distinguish 4 prognostic groups: favorable risk, good intermediate risk, adverse intermediate risk, and high risk. The corresponding 3-year OS rates were 39.5%, 30%, 10.6%, and 3.3%, respectively. The risk model allows further stratification of patients with intermediate-risk karyotype into 2 prognostic groups with implications for the therapeutic strategy.
XrML -- eXtensible rights Markup Language (2002)

XrML (eXtensible rights Markup Language) is an XML-based language for digital rights management (DRM), providing a universal method for specifying rights and conditions associated with the use and protection of digital content and services. Originally developed at Xerox's Palo Alto Research Center (PARC), the specification facilitates the creation of an open architecture for digital rights management of content or services. It can be integrated with both existing and new DRM systems. XrML is a general-purpose rights language, agnostic to the type of resource, platform, media, or business applications. The latest release, XrML 2.0, expands the capabilities of a digital rights language to enable developers to establish the rights and conditions needed to access web services in addition to digital content. It also contains additional capabilities in the areas of extensibility, security, and life cycle management. Recent actions in several standards bodies, most notably MPEG, OeBF and OASIS, have positioned XrML to become the world wide industry standard for a digital rights language. This paper provides a brief introduction to XrML, its data model, usage and extensibility as it applies to digital media, multi-tier content distribution and XML-based healthcare information.
Cheese Making in France and Italy (1967)

This article is based on a lecture given by Dr. Frank Kosikowski at the Fourth Annual Mar schall Invitational Italian Cheese Seminar, held in April 1967 at Madison, Wisconsin. Dr. Kosi kowski, an international authority on cheeses and cheese making, is the author of "Cheese and Fermented Milk Foods" ( Edward Brothers, Inc., Ann Arbor, Michigan), published in 1966.
Estradiol differentially regulates lipocalin-type prostaglandin D synthase transcript levels in the rodent brain: Evidence from high-density oligonucleotide arrays and<i>in situ</i>hybridization (2002)

Microarrays comprise an efficient approach to discovering large numbers of differentially expressed mRNA transcripts in the CNS resulting from changes in hormonal milieu. We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E(2)) on the transcriptomes from the medial basal hypothalamus and other brain regions of E(2)-treated (10 microg) adult female mice. Our results have revealed several unanticipated gene regulations. Most striking is lipocalin prostaglandin D(2) synthase (L-PGDS), which catalyzes the conversion of prostaglandin (PG) H(2) to PGD(2), a neuromodulator involved in a variety of functions, including sleep, pain, and odor responses. In situ hybridization revealed significant increases in L-PGDS expression in the arcuate and ventromedial nucleus of the medial basal hypothalamus compared with vehicle controls. The magnitude of these changes is approximately 2-fold and suggests a modulatory role for PGD(2) in E(2)-controlled neuroendocrine secretions and behaviors. Surprisingly, L-PGDS gene expression is reduced 2-fold after E(2) treatment in the ventrolateral preoptic area (VLPO), the suspected site of action for the sleep-promoting effects of PGD(2). Finally, whereas L-PGDS has been reported to be expressed primarily in oligodendrocytes of the adult rodent brain, we demonstrate, immunocytochemically, that L-PGDS is also expressed in a population of VLPO neurons. Thus, our data suggest the intriguing possibility that E(2) modulation of L-PGDS plays a role in the regulation of sleep-wake states through hitherto unknown mechanisms in VLPO neurons and through hormone-dependent neuronal-glial cooperation.
MR Imaging of the Breast for the Detection, Diagnosis, and Staging of Breast Cancer (2001)

With the introduction of contrast agents, advances in surface coil technology, and development of new imaging protocols, contrast agent-enhanced magnetic resonance (MR) imaging has emerged as a promising modality for detection, diagnosis, and staging of breast cancer. The reported sensitivity of MR imaging for the visualization of invasive cancer has approached 100%. There are many examples in the literature of MR imaging--demonstrated mammographically, sonographically, and clinically occult breast cancer. Often, breast cancer detected on MR images has resulted in a change in patient care. Despite these results, there are many unresolved issues, including no defined standard technique for contrast-enhanced breast MR imaging, no standard interpretation criteria for evaluating such studies, no consensus on what constitutes clinically important enhancement, and no clearly defined clinical indications for the use of MR imaging. Furthermore, this technology remains costly, and issues of cost-effectiveness and cost competition from percutaneous biopsy have yet to be fully addressed. These factors along with the lack of commercially available MR imaging--guided localization and biopsy systems have slowed the transfer of this imaging technology from research centers to clinical breast imaging practices. Technical requirements, potential clinical applications, and potential pitfalls and limitations of contrast-enhanced MR imaging as a method to help detect, diagnose, and stage breast cancer will be described.
A Monomer-Dimer Equilibrium of a Cellular Prion Protein (PrPC) Not Observed with Recombinant PrP (2000)

Both the purified normal (protease-sensitive) isoform of the prion protein (PrP(C)) (Pergami, P., Jaffe, H., and Safar, J. (1996) Anal. Biochem. 236, 63-73) and recombinant prion protein (PrP) have been found to be in monomeric form (Mehlhorn, I., Groth, D., Stockel, J., Moffat, B., Reilly, D., Yansura, D., Willet, W. S., Baldwin, M., Fletterick, R., Cohen, F. E., Vandlen, R., Henner, D., and Prusiner, S. B. (1996) Biochemistry 35, 5528-5537; and this paper), and therefore PrP(C)-PrP(C) interactions were previously unknown. In this report we confirm recombinant PrP to be a monomer by analytical ultracentrifugation. However, by three lines of evidence (enzyme-linked immunosorbent assay (ELISA), cross-linking experiments, and size exclusion chromatography) we could also demonstrate that, under native conditions, at least part of the native bovine PrP(C) exists as a monomer-dimer equilibrium. A bovine PrP(C)-specific immuno-sandwich ELISA was developed and calibrated with recombinant PrP (Meyer, R. K., Oesch, B., Fatzer, R., Zurbriggen, A., and Vandevelde, M. (1999) J. Virol. 73, 9386-9392). By this ELISA we identified a distinct PrP(C) fraction and partially purified this protein. When serial dilutions of brain homogenate or partially purified PrP(C) were measured, using the peptide antibody C15S, a nonlinear dose-response curve was obtained. This nonlinearity was shown not to be due to an artifact of the procedure but to a monomer-dimer equilibrium of PrP(C) with preferential binding of the antibody to the dimer. From the curvature we could deduce the association constant (3.9 x 10(8) M(-1) at 37 degrees C). Accordingly, DeltaG degrees of the reaction was calculated (-48.6 kJ M(-1)), and DeltaH degrees (9.5 kJ M(-1)) as well as DeltaS degrees (0.2 kJ K(-1) M(-1)) were extrapolated from the van't Hoff plot. When serial dilutions of monomeric recombinant PrP were tested, only a straight line was obtained, supporting our hypothesis. Additional evidence of dimer formation was revealed by Western blotting of partially purified PrP(C) cross-linked by the homobifunctional cross-linker BS(3). Finally, size exclusion chromatography of partially purified PrP(C) fractions revealed an additional shoulder not observed with recombinant PrP. The difference in respect of dimer formation between native PrP(C) and recombinant PrP could be explained by the lack of glycosylation of the latter.
A Frequency-Controlled Beam-Steering Array With Mixing Frequency Compensation for Multichannel Applications (2004)

A frequency-controlled beam-steering planar array with mixing frequency compensation is presented for cost-effective multichannel phased array applications. The new feed networks for frequency compensation not only operate in wide band but also ensure radio-frequency (RF) amplitude imbalance cancellation and progressive phase distribution. The parallel equal power dividers installed in both LO and intermediate-frequency (IF) feed networks provide uniform amplitude and phase distribution, while the fixed delay lines installed in the LO feed network exhibit precise phase progression, compared to a series feed structure. The LO power imbalance caused by the unequal delay line loss between elements is corrected by pumping each mixer into the LO saturation region, leading to linear IF-RF response. Thus, sidelobe degradation and pattern distortion caused by the RF amplitude imbalance, as well as the beam-steering error and beam squint caused by the phase errors of the delay lines, are removed. The proposed feed networks combined with quasi-Yagi antenna arrays and microwave monolithic integrated circuit mixers realize a broad bandwidth of more than 3 GHz in K band for multichannel wireless applications. The K band transmitter/receiver pair proposed in this paper successfully demonstrates two-channel simultaneous RF transmission and single channel 50-Mb/s data communication with 40/spl deg/ scanning. This simple, compact, yet cost-effective planar array ensures multichannel broadband wireless communications with beam-steering capability.
A 20-ns 256 K*4 FIFO memory (1988)

A 256 K*4 FIFO (first-in-first-out) CMOS memory with 20-ns access time and 30-ns cycle time is described. To accomplish full static and asynchronous operation, signal synchronizer and arbiter circuits have been developed and implemented into the device. A pair of 120-word*4-bit static memories are furnished to provide 20-ns data access from the very first read cycle. The average current measured at 30-ns read/write operation and the standby current are typically 23 and 1.2 mA, respectively.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Neuroinflammation: the devil is in the details (2016)

Abstract There is significant interest in understanding inflammatory responses within the brain and spinal cord. Inflammatory responses that are centralized within the brain and spinal cord are generally referred to as ‘neuroinflammatory’. Aspects of neuroinflammation vary within the context of disease, injury, infection, or stress. The context, course, and duration of these inflammatory responses are all critical aspects in the understanding of these processes and their corresponding physiological, biochemical, and behavioral consequences. Microglia, innate immune cells of the CNS , play key roles in mediating these neuroinflammatory responses. Because the connotation of neuroinflammation is inherently negative and maladaptive, the majority of research focus is on the pathological aspects of neuroinflammation. There are, however, several degrees of neuroinflammatory responses, some of which are positive. In many circumstances including CNS injury, there is a balance of inflammatory and intrinsic repair processes that influences functional recovery. In addition, there are several other examples where communication between the brain and immune system involves neuroinflammatory processes that are beneficial and adaptive. The purpose of this review is to distinguish different variations of neuroinflammation in a context‐specific manner and detail both positive and negative aspects of neuroinflammatory processes. image In this review, we will use brain and spinal cord injury, stress, aging, and other inflammatory events to illustrate the potential harm and benefits inherent to neuroinflammation. Context, course, and duration of the inflammation are highly important to the interpretation of these events, and we aim to provide insight into this by detailing several commonly studied insults. This article is part of the 60th anniversary supplemental issue .
Human subcommissural organ, with particular emphasis on its secretory activity during the fetal life (2001)

The subcommissural organ (SCO) is a conserved brain gland present throughout the vertebrate phylum. During ontogeny, it is the first secretory structure of the brain to differentiate. In the human, the SCO can be morphologically distinguished in 7- to 8-week-old embryos. The SCO of 3- to 5-month-old fetuses is an active, secretory structure of the brain. However, already in 9-month-old fetuses, the regressive development of the SCO-parenchyma is evident. In 1-year-old infants, the height of the secretory ependymal cells is distinctly reduced and they are grouped in the form of islets that alternate with cuboid non-secretory ependyma. The regression of the SCO continues during childhood, so that at the ninth year of life the specific secretory parenchyma is confined to a few islets of secretory ependymal cells. The human fetal SCO shares the distinct ultrastructural features characterizing the SCO of all other species, namely, a well-developed rough endoplasmic reticulum, with many of its cisternae being dilated and filled with a filamentous material, several Golgi complexes, and secretory granules of variable size, shape, and electron density. The human fetal SCO does not immunoreact with any of the numerous polyclonal and monoclonal antibodies raised against RF-glycoproteins of animal origin. This and the absence of RF in the human led to the conclusion that the human SCO does not secrete RF-glycoproteins. Taking into account the ultrastructural, lectin-histochemical, and immunocytochemical findings, it can be concluded that the human SCO, and most likely the SCO of the anthropoid apes, secrete glyco- protein(s) with a protein backbone of unknown nature, and with a carbohydrate chain similar or identical to that of RF-glycoproteins secreted by the SCO of all other species. These, as yet unidentified, glycoprotein(s) do not aggregate but become soluble in the CSF. Evidence is presented that these CSF-soluble proteins secreted by the human SCO correspond to (1) a 45-kDa compound similar or identical to transthyretin and, (2) a protein of about 500 kDa.
Symbol-Wise Beamforming with Limited Feedback for MIMO-OFDM Systems (2011)

In this paper, we consider the symbol-wise beamforming with limited feedback for MIMO-OFDM systems in the presence of co-channel interference. We propose a non-iterative algorithm for the symbol-wise beamforming with limited feedback, which provides the considerable performance with low computational complexity. We also propose the codebook for the symbol-wise beamforming with limited feedback under spatially correlated frequency-selective channel. It is clear from our simulation that our proposed scheme outperforms the symbol-wise beamforming with limited feedback based on the iterative algorithm. We also confirm that our proposed codebook shows a better performance compared to the random vector quantization (RVQ) codebook.
Effect of Laser Preheating the Workpiece on Micro-End Milling of Metals (2005)

Micro-end milling is a fast and direct method of creating net-shaped functional micro parts, micro molds, and prototypes. However, the materials that can be micro machined efficiently are limited by the small flexural stiffness, strength, and hardness of the tool. The small flexural stiffness and strength of micro-end mills limits the size of cut that can be taken and the productivity. The flexural stiffness and strength are a strong function of the tool diameter. It is not expected that in the near term a new material will be developed that offers a combination of hardness and yield strength that will result in a significantly improved strength for tools with diameters measured in the 10s and 100s of microns. To enable a significant increase in performance requires higher spindle speeds and increased chip loads. Laser-assisted micro-end milling has the potential to increase the chip load and productivity by reducing the yield strength at the cutting location. This study examines the effect of laser preheating on micro-end milling of 6061-T6 aluminum and 1018 steel. 300 micron-diameter, two-flute, carbide end mills are used to cut 85 micron-deep slots at a constant 40,000 rpm spindle speed. The laser power and chip load are varied, including no preheating, to show their effect on cutting forces, specific cutting energy, burr formation, surface finish, and temperature. The results are related to average material removal temperature by predictions from a heat transfer model of the workpiece undergoing laser preheating. Results indicate that chip load and productivity can be significantly increased during dry machining of 6061-T6 aluminum and 1018 steel by locally preheating the workpiece immediately ahead of the micro-end mill.