gebhart/patrepeval huggingface dataset:
Nonvolatile static random access memory based on spin-transistor architecture (2009)

The authors proposed and computationally analyzed nonvolatile static random access memory (NV-SRAM) architecture using a new type of spin transistor comprised of a metal-oxide-semiconductor field-effect transistor (MOSFET) and magnetic tunnel junction (MTJ) that is referred to as a pseudo-spin-MOSFET (PS-MOSFET). The PS-MOSFET is a circuit approach to reproduce the functions of spin transistors, based on recently progressed magnetoresistive random access memory technology. The proposed NV-SRAM cell can be simply configured by connecting two PS-MOSFETs to the storage nodes of a standard SRAM cell.
Spectrometric Study on the Interaction of Dodecyltrimethylammonium Bromide with Curcumin (2011)

The interaction between dodecyltrimethylammonium bromide (DTAB) and curcumin has been studied in pH 5.0 sodium phosphate buffer using absorption and fluorescence measurements. With increasing DTAB concentration (C(DTAB)) from 0 to 20 mM, the absorption peak of curcumin at 430 nm, corresponding to the conjugated structure of curcumin, first weakens gradually into a shoulder but increases back into one peak with much higher absorption intensity. On the contrary, as C(DTAB) increases, the initial small absorption shoulder of curcumin at 355 nm, corresponding to the feruloyl unit of curcumin, first increases gradually into a clear peak but decreases back into one shoulder until almost disappeared finally. By remaining at nearly the same wavelength, the fluorescence of curcumin first decreases at C(DTAB) lower than 5 mM and then increases gradually up to C(DTAB) = 10 mM, which is followed by sharp increases of fluorescence intensity with marked blue-shifts at higher C(DTAB). The values of anisotropy and microviscosity of curcumin obtained from the fluorescence polarization technique also showed pronounced changes at different surfactant concentrations. The interaction mechanisms of DTAB with curcumin have been presented at low, intermediate, and high surfactant concentrations, which is relating to interaction forces, surfactant aggregations, as well as structural alterations of curcumin.
Laboratory Diagnosis of Mycobacterial Infections: New Tools and Lessons Learned (2001)

Even in the 21st century, tuberculosis continues to be a problem. Although the number of cases continues gradually to decrease in the United States, cases get more difficult to treat, specifically those that are multiple-drug resistant. Infection of one-third of the world's population ensures that tuberculosis will not disappear in the near future. In light of this, it will be useful to know the goals for the health care system and how these goals may be accomplished. Laboratory testing in the mycobacteriology field is experiencing more changes today than ever before. Determining what assays will be most useful to the clinician is a challenge, and acceptance of the new technology by the medical community an even greater one. Clinicians must use the best available resources to determine the most appropriate care for their patients and work together with the laboratory to ensure that the communication channels are open. This review focuses on current state-of-the-art resources useful for accurate and rapid laboratory diagnosis of mycobacterial infections.
A cohort mortality study of forestry workers exposed to phenoxy acid herbicides. (1991)

A cohort mortality study was undertaken of forestry workers at a public electrical utility who had worked for six months or more during 1950-82 and who were routinely exposed to herbicides including phenoxy acids. A total of 1222 men with 25 274 years at risk experienced 80 deaths. Ascertainment of vital state at the end of follow up was 95.5%. The male population of the province (Ontario) was used as the reference group. Overall, no excess mortality was found in this cohort relative to the reference population. A statistically significant increase in deaths occurred, however, due to suicide (SMR = 210, 95% confidence interval, 95% CI 105-376) for the cohort as a whole. No deaths were seen due to cancers such as soft tissue sarcoma and non-Hodgkin's lymphoma that have been cited by other authors as being associated with exposure to phenoxy acid herbicides. Although the cohort is not large, the absence of deaths due to these cancers is consistent with findings from other studies with sufficient numbers to allow examination of specific risks. The cohort is still young, however, and at the end of follow up most had not reached an age when increased incidence of cancer would normally be expected.
Genomic organization and chromosomal localization of the TAPA-1 gene (1991)

TAPA-1 is a 26-kDa integral membrane protein expressed on many human cell types. Antibodies against TAPA-1 induce homotypic aggregation of cells and can inhibit their growth. The murine homologue of TAPA-1 was cloned from both cDNA and genomic DNA libraries. A very high level of homology was found between human and mouse TAPA-1. The 5' untranslated region of the TAPA-1 gene resembles housekeeping gene promoters with respect to G + C content and the presence of potential Sp1 binding sites. The chromosomal localization of human and murine TAPA-1 genes was determined by Southern blot experiments using DNA from somatic cell hybrids. The genes were found to be part of a conserved syntenic group in mouse chromosome 7 and the short arm of human chromosome 11. The organization of the TAPA-1 gene and the projection of the exon boundaries on the proposed protein structure are presented.
DAMN: a distributed architecture for mobile navigation (1997)

An architecture is presented in which distributed taskachieving modules, or behaviors, cooperatively determine a mobile robot's path by voting for each of various possible actions. An arbiter then performs command fusion and selects that action which best satisfies the prioritized goals of the system, as expressed by these votes, without the need to average commands. Command fusion allows multiple goals and constraints to be considered simultaneously. Examples of implemented systems are given, and future research directions in command fusion are discussed.
Enzyme-linked immunosorbent assay for rubella immunoglobulin G: new method for attachment of antigens to microtiter plates (1983)

Many of the enzyme-linked immunosorbent assay (ELISA) techniques previously described for detection of rubella-specific antibodies employ complex technology not available in routine diagnostic laboratories. The method described allows the use of commercially available rubella hemagglutination inhibition (HI) antigen. Passive adsorption of these antigens to plastic is variable, but with the use of albumin as a bridge, it is possible to attach the antigen reliably to the plastic wells. Over 1,500 sera were tested by both HI and ELISA techniques to detect the presence of rubella antibodies. These sera were selected with a bias towards those with low levels of rubella-specific antibody, since it has been demonstrated that it is in this range that discrepancies are more likely to occur between HI and ELISA techniques. In 99% of the sera tested, the results of both techniques were in agreement. On the basis of these results, the technique offers a useful alternative to the routine rubella HI test and other ELISA techniques which need sophisticated antigen preparations.
Regulation of Human Vascular Endothelial Growth Factor mRNA Stability in Hypoxia by Heterogeneous Nuclear Ribonucleoprotein L (1999)

A 126-base region of human vascular endothelial growth factor (VEGF) 3'-untranslated region, which we identified as the hypoxia stability region, forms seven hypoxia-inducible RNA-protein complexes with apparent molecular masses ranging from 40 to 90 kDa in RNA-UV-cross-linking assays. In this study, we show that proteins that form the 60-kDa RNA-protein complex with the hypoxia stability region were present in both cytoplasmic and nuclear compartments. We purified the protein associated in the 60-kDa complex and identified it as heterogeneous nuclear ribonucleoprotein L (hnRNP L) by protein sequencing. Removal of hnRNP L by immunoprecipitation specifically abolished formation of the 60-kDa complex. Synthetic deoxyribonucleotide competition studies defined the RNA-binding site of hnRNP L as a 21-base-long sequence, 5'-CACCCACCCACAUACAUACAU-3'. Immunoprecipitation of hnRNP L followed by reverse transcription-polymerase chain reaction showed that hnRNP L specifically interacts with VEGF mRNA in hypoxic cells in vivo. Furthermore, when M21 cells transfected with antisense oligodeoxyribonucleotide to the hnRNP L RNA-binding site, the VEGF mRNA half-life was significantly reduced under hypoxic conditions. Thus, we propose that specific association of hnRNP L with VEGF mRNA under hypoxia may play an important role in hypoxia-induced post-transcriptional regulation of VEGF mRNA expression.
Acoustic emission from phase transition of some chemicals (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAcoustic emission from phase transition of some chemicalsTsuguo. Sawada, Yohichi. Gohshi, Chikako. Abe, and Keiichi. FuruyaCite this: Anal. Chem. 1985, 57, 8, 1743–1745Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/ac00285a054https://doi.org/10.1021/ac00285a054research-articleACS PublicationsRequest reuse permissionsArticle Views130Altmetric-Citations25LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Polymerase chain reaction amplification of bovine β‐lactoglobulin genomic sequences and identification of genetic variants by RFLP analysis (1990)

Abstract Genetic variants A and B of the bovine β‐lactoglobulin are common in all breeds of cattle and β‐lactoglobulin is an important factor in protein recovery and cheese yield from milk. Early and accurate identification of β‐lactoglobulin genotypes in animals of both sexes could have a direct impact on the genetic improvement of milk composition. We report a rapid method for differentiating bovine β‐lactoglobulin AA, AB or BB genotypes by using the polyrnerase chain reaction technique and restriction fragment length polymorphism.
Anisotropic Field-of-Views in Radial Imaging (2008)

Radial imaging techniques, such as projection-reconstruction (PR), are used in magnetic resonance imaging (MRI) for dynamic imaging, angiography, and short-T(2) imaging. They are robust to flow and motion, have diffuse aliasing patterns, and support short readouts and echo times. One drawback is that standard implementations do not support anisotropic field-of-view (FOV) shapes, which are used to match the imaging parameters to the object or region-of-interest. A set of fast, simple algorithms for 2-D and 3-D PR, and 3-D cones acquisitions are introduced that match the sampling density in frequency space to the desired FOV shape. Tailoring the acquisitions allows for reduction of aliasing artifacts in undersampled applications or scan time reductions without introducing aliasing in fully-sampled applications. It also makes possible new radial imaging applications that were previously unsuitable, such as imaging elongated regions or thin slabs. 2-D PR longitudinal leg images and thin-slab, single breath-hold 3-D PR abdomen images, both with isotropic resolution, demonstrate these new possibilities. No scan time to volume efficiency is lost by using anisotropic FOVs. The acquisition trajectories can be computed on a scan by scan basis.
The additive and synergistic antimicrobial effects of select frankincense and myrrh oils - a combination from the pharaonic pharmacopoeia (2012)

Frankincense and myrrh essential oils have been used in combination since 1500 bc; however, no antimicrobial investigations have been undertaken to confirm their effect in combination. This study validates the enhanced efficacy when used in combination against a selection of pathogens.
Subchondral pre-solidified chitosan/blood implants elicit reproducible early osteochondral wound-repair responses including neutrophil and stromal cell chemotaxis, bone resorption and repair, enhanced repair tissue integration and delayed matrix deposition (2013)

Pre-solidified implants elicit stromal cells and vigorous bone plate remodeling through a phase involving neutrophil chemotaxis. Pre-solidified chitosan implants are tunable by molecular mass, and could be beneficial for augmented marrow stimulation therapy if the recruited stromal cells can progress to bone and cartilage repair.
IoTrace: A Flexible, Efficient, and Privacy-Preserving IoT-Enabled Architecture for Contact Tracing (2021)

Contact tracing promises to help fight the spread of COVID-19 via an early detection of possible contagion events. To this end, most existing solutions share the following architecture: smartphones continuously broadcast random beacons that are intercepted by nearby devices and stored into their local contact logs. In this article, we propose an IoT-enabled architecture for contact tracing that relaxes the smartphone-centric assumption, and provides a solution that enjoys the following features: it reduces the overhead on the end user to the bare minimum - the mobile device only broadcasts its beacons; it provides the user with a degree of privacy not achieved by competing solutions - even in the most privacy adverse scenario, the solution provides k-anonymity; and it is flexible: the same architecture can be configured to support several models - ranging from fully decentralized to fully centralized ones - and the system parameters can be tuned to support the tracing of several social interaction models. What is more, our proposal can also be adopted to tackle future human-proximity transmissible diseases. Finally, we also highlight open issues and discuss a number of future research directions at the intersection of IoT and contact tracing.
Fab Integrated Packaging (FIP): a new concept for high reliability wafer-level chip size packaging (2000)

Wafer Level Packaging has the highest potential for future single chip packages. The package is completed directly on the wafer then singulated by dicing for the assembly in a flip chip fashion. All packaging and testing operations of simulated dice will be replaced by whole wafer fabrication and wafer level testing. The result is a technology which leads the way to Fab Integrated Packaging (FIP). An evaluation of the reliability of a new Wafer-Level Chip Scale Package (WL-CSP) was done in the FIP program, a joint development program between Fraunhofer IZM and Motorola. As a CSP the FIP-CSP eliminates underfill operation during flip-chip bonding using high through-put SMT assembly lines. The technological structure of this FIP-CSP is a pad redistributed die with a solder ball array. A stress compensation layer (SCL) embeds the solder balls before second solder balls are stencil printed or placed on top of embedded balls. The reliability of this wafer-level CSP presented here was simulated and evaluated by test samples. The test chip was a 1 cm/spl times/1 cm square chip which was redistributed to an 14/spl times/14 ball array with a pitch of 0.5 mm. JEDEC Level 3, 1000 cycles AATC (-55/spl deg/C/+125/spl deg/C) and 48 h Autoclave on component level were passed. On board level 1000 hours humidity storage at 85/spl deg/C (85/85 test) and 1000 cycles -55/+125/spl deg/C were passed.
Circulatory Assistance With a Permanent Implantable IABP: Initial Human Experience (2002)

Mean bypass time was 157 minute (range 120 to 196 minute); mean cross-clamp time was 101 minute (range 69 to 144). Patient 1 died intra-operatively. Compared with preoperative values, at 1 month, cardiac index increased (1.7 to 2.6 L/min/m(2)) and there were significant decreases in creatinine (2.6 to 1.5 mg/dL), pulmonary capillary wedge pressure (PCWP) (32 to 14 mm Hg), and right atrial pressure (RA) (19 to 9 mm Hg). NYHA class improved (IV to II). The mean increase in cardiac index with the KCV OFF to ON was 0.53 L/min/m(2) (36%). Two patients were discharged home. The device was used intermittently without thromboembolic complications. The only device related complications were attributed to PAD design and have been corrected. CONCLUSION Our initial human trial demonstrates successful implantation of the KCV in end-stage patients, the ability of the device to be used intermittently without anticoagulation, and documents hemodynamic and functional improvement in the status of these patients.
Chronic Thromboembolic Pulmonary Hypertension (CTEPH) – Potential Role of Multidetector-Row CT (MD-CT) and MR Imaging in the Diagnosis and Differential Diagnosis of the Disease (2014)

Chronic thromboembolic pulmonary hypertension (CTEPH) can be defined as pulmonary hypertension (resting mean pulmonary arterial pressure of 25 mm Hg or more determined at right heart catheterization) with persistent pulmonary perfusion defects. It is a rare, but underdiagnosed disease with estimated incidences ranging from 0.5% to 3.8% of patients after an acute pulmonary embolism (PE), and in up to 10% of those with a history of recurrent PE. CTEPH is the only form of pulmonary hypertension that can be surgically treated leading to normalization of pulmonary hemodynamics and exercise capacity in the vast majority of patients. The challenges for imaging in patients with suspected CTEPH are fourfold: the imaging modality should have a high diagnostic accuracy with regard to the presence of CTEPH and allow for differential diagnosis. It should enable detection of patients suitable for PEA with great certainty, and allow for quantification of PH by measuring pulmonary hemodynamics (mPAP and PVR), and finally, it can be used for therapy monitoring. This overview tries to elucidate the potential role of ECG-gated multidetector CT pulmonary angiography (MD-CTPA) and MR imaging, and summarizes the most important results that have been achieved so far. Generally speaking, ECG-gated MD-CTPA is superior to MR in the assessment of parenchymal and vascular pathologies of the lung, and allows for the assessment of cardiac structures. The implementation of iodine maps as a surrogate for lung perfusion enables functional assessment of lung perfusion by CT. MR imaging is the reference standard for the assessment of right heart function and lung perfusion, the latter delineating typical wedge-shaped perfusion defects in patients with CTEPH. New developments show that with MR techniques, an estimation of hemodynamic parameters like mean pulmonary arterial pressure and pulmonary vascular resistance will be possible. CT and MR imaging should be considered as complementary investigations providing comprehensive information in patients with CTEPH.
Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands (2009)

One limitation in the development of biosensors for the early detection of disease is the availability of high specificity and affinity ligands for biomarkers that are indicative of a pathogenic process. Within the past 10 years, biopanning of phage displayed peptide libraries on intact cells has proven to be a successful route to the identification of cell-specific ligands. The peptides selected from these combinatorial libraries are often able to distinguish between diseased cells and their normal counterparts as well as cells in different activation states. These ligands are small and chemical methodologies are available for regiospecific derivatization. As such, they can be incorporated into a variety of different diagnostic and therapeutic platforms. Here we describe the methods utilized in the selection of peptides from phage displayed libraries by biopanning. In addition, we provide methods for the synthesis of the selected peptides as both monomers and tetramers. Downstream uses for the peptides are illustrated.
Stay off the lawn - Creating smooth paths based on region preferences (2013)

Modern virtual environments can contain a variety of characters and traversable regions. Each character may have different preferences for the traversable region types. Pedestrians may prefer to walk on sidewalks, but they may occasionally need to traverse roads and dirt paths. By contrast, wild animals might try to stay in forest areas, but they are able to leave their protective environment when necessary. This paper presents a novel path planning method named MIRAN (Modified Indicative Routes and Navigation) that takes a character’s region preferences into account. Given an indicative route as a rough estimation of a character’s preferred route, MIRAN efficiently computes a visually convincing path that is smooth, keeps clearance from obstacles, avoids unnecessary detours, and allows local changes to avoid other characters. To the best of our knowledge, MIRAN is the first path planning method that supports the above features while using an exact representation of the navigable space. Experiments show that with our approach a wide range of different character behaviors can be simulated. It also overcomes problems that occur in previous path planning methods such as the Indicative Route Method. The resulting paths are well-suited for real-time simulations and gaming applications.
Biological Properties of Conjugates of Mitomycin C with Estradiol Benzoate and Estradiol: Their Stability Characteristics in Biological Media and Their Binding Abilities to Estrogen Receptor. (1997)

Conjugates of mitomycin C (MMC) with estradiol benzoate and estradiol via glutaric acid, abbreviated to EB-glu-MMC and E-glu-MMC, respectively, were investigated in vitro to determine their stability and MMC regeneration properties in biological media and on their binding to estrogen receptor. EB-glu-MMC and E-glu-MMC were added into a mixture of 1/15 M phosphate buffer, pH 7.4 (ionic strength = 0.3), propylene glycol (PG) and rat plasma (4:5:1, v/v/v), named 10% plasma, or into a mixture of the buffer, PG and rat liver homogenate (9:10:1, v/v/w), named 5% liver homogenate, and each was incubated at 37 degrees C. The conversion characteristics of EB-glu-MMC and E-glu-MMC were compared with those in the buffer-PG (1:1, v/v) mixture previously reported. In 10% plasma, the change of EB-glu-MMC to E-glu-MMC was accelerated enzymatically to some extent, while the enzymatic degradation of E-glu-MMC was not accelerated at all. In 5% liver homogenate, EB-glu-MMC changed quickly to E-glu-MMC, whereas the degradation of E-glu-MMC was accelerated very little. E-glu-MMC was considered to be rather stable against enzyme in the biological media. Competitive binding studies using the rat uterine estrogen receptor showed that the specific binding affinity of E-glu-MMC was 0.81% to that of estradiol, while EB-glu-MMC hardly exhibited specific binding. E-glu-MMC was regarded as a hormone-drug conjugate showing a small specific binding affinity to the estrogen receptor. E-glu-MMC is considered to be an effective antitumor agent which gradually generates MMC in the body, and its receptor-mediated action to target cells such as estrogen receptor-positive tumor cells might be possible.