gebhart/patrepeval huggingface dataset:
Ex Utero Culture and Live Imaging of Mouse Embryos (2011)

Mouse genetic approaches when combined with live imaging tools are revolutionizing our current understanding of mammalian developmental biology. The availability and improvement of a wide variety of genetically encoded fluorescent proteins have provided indispensable tools to visualize cells and subcellular features in living organisms. It is now possible to generate genetically modified mouse lines expressing several spectrally distinct fluorescent proteins in a tissue-specific or -inducible manner. Such reporter-expressing lines make it possible to image dynamic cellular behaviors in the context of living embryos undergoing normal or aberrant development. As with all viviparous mammals, mouse embryos develop within the uterus, and so live imaging experiments require culture conditions that closely mimic the in vivo environment. Over the past decades, significant advances have been made in developing conditions for culturing both pre- and postimplantation-stage mouse embryos. In this chapter, we discuss routine methods for ex utero culture of preimplantation- and postimplantation-stage mouse embryos. In particular, we describe protocols for collecting mouse embryos of various stages, setting up culture conditions for their ex utero culture and imaging, and using laser scanning confocal microscopy to visualize live processes in mouse embryos expressing fluorescent reporters.
Real‐time control of shunt active power filter under distorted grid voltage and unbalanced load condition using self‐tuning filter (2014)

In this paper, an alternative control method is proposed to improve the harmonic suppression efficiency of the active power filter in a distorted and an unbalanced power system to compensate for the perturbations caused by the unbalanced non‐linear loads. The proposed method uses a self‐tuning filter (STF) to process the grid voltage in order to provide a uniform reference voltage to obtain the correct angular position of the phase locked loop. Moreover, the required compensation currents are obtained by implementing another STF in the transformed set of currents in order to separate the fundamental and the harmonic currents. This allows the calculation of a precise reference current for the unbalanced, the non‐linear and the variable load conditions. The proposed control method gives an adequate compensating current reference even for a non‐ideal voltage and unbalanced current conditions. The real‐time control of the filter under the distorted and the unbalanced power system is developed in an RT‐LAB real‐time platform. The results obtained in the software‐in‐the‐loop configuration are presented to verify the effectiveness of the proposed control technique.
Decay of the New Nuclide<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">Ne</mml:mi></mml:mrow><mml:mrow><mml:mn>24</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math> (1956)

The nuclide ${\mathrm{Ne}}^{24}$ has been produced by bombarding a neon gas target with 1.5-Mev tritons, the reaction being ${\mathrm{Ne}}^{22}(t, p){\mathrm{Ne}}^{24}$. Beta and gamma scintillation spectrometers with coincidence circuits were used to study this nuclide. ${\mathrm{Ne}}^{24}$ decays with a half-life of 3.38\ifmmode\pm\else\textpm\fi{}0.02 min, emitting negative beta groups of 1.98\ifmmode\pm\else\textpm\fi{}0.05 and 1.10\ifmmode\pm\else\textpm\fi{}0.05 Mev. This decay is also accompanied by gamma rays of 472\ifmmode\pm\else\textpm\fi{}5 and 878\ifmmode\pm\else\textpm\fi{}9 kev with relative intensities of 100 to 8, respectively. The excited levels in ${\mathrm{Na}}^{24}$ corresponding to the above transitions are at 0.472 and 1.35 Mev. The 0.472-Mev level is an isomeric state with a half-life of the order of 20 milliseconds. The spins and parities of the ${\mathrm{Na}}^{24}$ ground state, 0.472-Mev, and 1.35-Mev levels are 4+, 1+, and 1+, respectively. The ${\mathrm{Ne}}^{24}$-${\mathrm{Na}}^{24}$ energy difference is 2.449\ifmmode\pm\else\textpm\fi{}0.035 Mev, and the ${\mathrm{Ne}}^{24}$ mass is 24.001195\ifmmode\pm\else\textpm\fi{}0.000040 atomic mass units.
Double blind, randomized controlled trial, to evaluate the effectiveness of a controlled nitric oxide releasing patch versus meglumine antimoniate in the treatment of cutaneous leishmaniasis [NCT00317629] (2006)

A double-blind, randomized, double-masked, placebo-controlled clinical trial, including 620 patients from endemic areas for Leishmaniasis in Colombia was designed to investigate whether this patch is as effective as meglumine antimoniate for the treatment of cutaneous leishmaniasis but with less adverse events. Subjects with ulcers characteristic of cutaneous leishmaniasis will be medically evaluated and laboratory tests and parasitological confirmation performed. After checking the inclusion/exclusion criteria, the patients will be randomly assigned to one of two groups. During 20 days Group 1 will receive simultaneously meglumine antimoniate and placebo of nitric oxide patches while Group 2 will receive placebo of meglumine antimoniate and active nitric oxide patches. During the treatment visits, the medications will be daily administered and the presence of adverse events assessed. During the follow-up, the research group will visit the patients at days 21, 45, 90 and 180. The healing process of the ulcer, the health of the participants, recidivisms and/or reinfection will also be assessed. The evolution of the ulcers will be photographically registered. In case that the effectiveness of the patches is demonstrated, a novel and safe therapeutic alternative for one of the most important public health problems in many countries will be available to patients.
Advantages and Disadvantages of Driving Simulators: A Discussion (2012)

For half a century at least, each new generation of computer chip, appearing every two years or so, has provided twice as many transistors per unit cost (as predicted by Moore’s law [1]). Extrapolating the accelerating pace of technological potential, logic dictates that computational devices will be tiny and powerful by the early 2020s, giving rise to virtual-reality applications such as displays built into our eyeglasses [2]. By the 2030s, going to a website could mean entering a totally realistic and compelling virtual environment facilitated by miniature computers that interact with brain cells, and people will probably spend most of their time in virtual reality [3]. Although these prospects may sound farfetched, we can already see that virtual-reality-based applications are increasingly used in such common tasks as driving. We use driving simulators for assessment, rehabilitation, learner driver training, race-car driver training, research into safety, and for at-home entertainment and in amusement halls.
Electrophoretic Characterization of the Human Sperm—Specific Enolase at Different Stages of Maturation (2004)

The presence of a sperm-specific enolase isoform (ENO-S) in human ejaculated spermatozoa was previously demonstrated. The objective of this study was to characterize this ENO-S in spermatozoa at different steps of maturation. Sperm ENO-S was characterized in testicular, epididymal, and ejaculated spermatozoa to determine whether any change occurred in the isoform patterns of this enzyme during epididymal maturation. In testicular sperm, ENO-S was present under 2 main bands named S1 and S3. In epididymal sperm, S1 and S3 bands and a prominent additional S2 band, with the same electrophoretic properties as the S isoform of ejaculated sperm, were visualized. In the testicular extracts obtained from testes in which no spermatozoa were visualized by histologic analysis, none of the 3 ENO-S bands was found. ENO-S exists as different isoforms (electrophoretic variants) in the different stages of sperm maturation. Passage through the epididymis seems to play a major role in the maturational process of this sperm-specific enolase.
A Dynamic Model of Customers’ Usage of Services: Usage as an Antecedent and Consequence of Satisfaction (1999)

As firms seek ways to manage customer relationships over the long term, understanding the dynamics of the service provider–customer relationship becomes a key priority. In this article, the authors develop and test a dynamic model of customer usage of services, identifying causal links between customer's prior usage levels, satisfaction evaluations, and subsequent service usage. The authors quantify the (heretofore anecdotal) relationship between customer satisfaction and subsequent service usage and provide new theoretical insights into the antecedents of customer satisfaction by introducing the concept of perceived payment equity. The primary contribution of the article is an understanding of how service usage changes over time, and particularly, how the consumer's view of the fairness or “equity” of the exchange over time affects the consumer's usage of services. Specifically, payment equity represents the customer's changing evaluation of the fairness of the level of economic benefits derived from usage in relation to the level of economic costs, that is, the customer's evaluation of the fairness of the exchange. By examining the antecedents of payment equity, it can be determined how customers use price and usage over time to update their evaluations of the fairness of the exchange. This evaluation affects overall satisfaction, which in turn affects future usage. The results suggest that customers’ usage levels can be managed through pricing strategies, communications, and more generally dynamic customer satisfaction management.
Visual support for version management (1992)

Article Free Access Share on Visual support for version management Authors: M. Wein National Research Council of Canada National Research Council of CanadaView Profile , Wm Cowan University of Waterloo University of WaterlooView Profile , W. M. Gentleman National Research Council of Canada National Research Council of CanadaView Profile Authors Info & Claims SAC '92: Proceedings of the 1992 ACM/SIGAPP symposium on Applied computing: technological challenges of the 1990'sMarch 1992 Pages 1217–1223https://doi.org/10.1145/130069.130151Published:01 March 1992Publication History 5citation241DownloadsMetricsTotal Citations5Total Downloads241Last 12 Months5Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Calcium-dependent chloride secretion across cultures of human tracheal surface epithelium and glands (1993)

Surface epithelium and gland cells from human trachea were cultured on porous-bottom inserts and loaded with fura 2 to permit measurement of the intracellular calcium concentration ([Ca2+]i). Short-circuit current (Isc), an index of transepithelial active ion transport, was measured on cells from the same cultures. Surface epithelial [Ca2+]i of 82 +/- 15 nM was increased transiently by isoproterenol, histamine, and bradykinin with maximal increases of 88 +/- 17, 480 +/- 149, and 978 +/- 214 nM (n = 15), respectively. Baseline [Ca2+]i in cultured gland cells of 68 +/- 11 nM was increased transiently by isoproterenol, histamine, methacholine, and bradykinin with maximal increases of 105 +/- 19, 233 +/- 47, 327 +/- 121, and 634 +/- 151 nM (n = 17-21), respectively. In both cell types, mediators that increased [Ca2+]i also increased Isc with a time course identical to the increase in [Ca2+]i. Pretreatment with the calcium chelator, 1,2-bis-(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid, acetoxymethyl ester (BAPTA-AM), had no effect on basal Isc or transepithelial resistance but markedly inhibited both the Isc and [Ca2+]i responses to agonists. Forskolin (10(-5) M), 3-isobutyl-1-methylxanthine (10(-3) M), dibutyryl adenosine 3',5'-cyclic monophosphate (10(-3) M), and 8-(4-chlorophenylthio)-cAMP (10(-3) M) had no or only trivial effects on Isc and [Ca2+]i. We suggest that mediators increase Isc across human airway epithelium by activating Ca-dependent basolateral K channels, resulting in hyperpolarization and an increased driving force for Cl exit through apical membrane Cl channels.
Integrated acoustooptic and magnetooptic devices for optical information processing (1996)

Acoustooptic (AO) Bragg interactions between guided optical waves and surface acoustic waves (SAW's) and the resulting AO Bragg cell modulators have evolved in integrated AO technology in recent years. A variety of hybrid multichannel integrated AO device modules including power and interferometric RF spectrum analyzers, space switches, tunable filters, frequency shifters and modulators, analog algebraic processors, programmable correlators and other multichannel device modules have been realized in common LiNbO/sub 3/ substrate up to 1.0/spl times/3.7 cm/sup 2/ in maximum size. The prospects for realization of their counterparts in monolithic integrated format in a common substrate of InGaAsP material system are also significantly enhanced recently. These AO device modules have shown a high degree of modularity and suggested applications in optical information processing and communications. In the meantime, the magnetooptic (MO) Bragg interactions between guided optical waves and magnetostatic waves (MSWs) in yttrium iron garnet-gadolinium gallium garnet (YIG-GGG) waveguides have resulted in miniaturized MO Bragg cell modulators in analogy with the guided-wave AO Bragg cell modulators. Most recently the performance of such MO Bragg cell modulators has been significantly improved and the modulators have been integrated with ion-milled waveguide lenses to realize hybrid integrated MO device modules, and have been utilized to demonstrate a number of novel applications. In this paper, a review of some of these integrated AO and MO device modules and applications together with some unpublished new results are presented.
Characterization and photoaffinity labeling of receptor sites for the Ca2+ channel inhibitors d-cis-diltiazem, (+/-)-bepridil, desmethoxyverapamil, and (+)-PN 200-110 in skeletal muscle transverse tubule membranes. (1986)

In order to further understand the molecular nature of the voltage-sensitive Ca2+ channel in skeletal muscle, we have performed classical radioligand binding studies and photoaffinity labeling with different types of tritiated inhibitors of the Ca2+ channel. The equilibrium dissociation constants (KD) for (-)-[3H]desmethoxyverapamil, d-cis-[3H]diltiazem, and (+/-)-[3H]bepridil at their receptor sites in skeletal muscle transverse tubule membranes are: 1.5 +/- 0.5, 50 +/- 5, and 20 +/- 5 nM, respectively. Maximum binding capacities in picomoles/milligram of protein were: 70 +/- 10 for (-)-[3H]desmethoxyverapamil, 50 +/- 15 for d-cis-[3H]diltiazem, and 75 +/- 15 for (+/-)-[3H]bepridil. The kinetics of association at 10 degrees C for the three types of tritiated compounds were relatively slow (3 X 10(5) M-1 S-1 for (-)-[3H]desmethoxyverapamil, 8 X 10(3) M-1 S-1 for d-cis-[3H]diltiazem, and 4.2 X 10(5) M-1 S-1 for (+/-)-[3H]bepridil). The dissociation of (-)-[3H]desmethoxyverapamil and d-cis-[3H]diltiazem from their receptor sites was also a slow process with half-lives of dissociation of 33 and 36 min, respectively. Competition studies using the three tritiated ligands suggest that they bind to the same receptor site which appears to be in a 1:1 stoichiometry with the dihydropyridine receptor. Photoaffinity labeling with high intensity ultraviolet light in the presence of (+/-)-[3H]bepridil or d-cis[3H]diltiazem resulted in the specific covalent incorporation of radioactivity into a polypeptide of Mr 170,000 +/- 10,000. A polypeptide of Mr 170,000 was also specifically labeled in photoaffinity labeling experiments using the high affinity dihydropyridine derivative (+)-[3H]PN 200-100.
SIP signaling in Mobile electronic Transaction (2002)

WAP and SIP are two different technologies to provide telephone services at present. Both technologies employ different approaches to establish connection, transmit data and terminate connection.\n\nThis thesis focuses on establishment of SIP signaling for Mobile electronic Transaction and tearing off the connections. WAP and SIP protocols have been analyzed and finally the possible compatibility and pair wise mapping between both have been discussed. Features of both the protocols have been discussed and uses of a service broker between them are outlined.
Oxidative Desulfurization:  Oxidation Reactivity of Sulfur Compounds in Different Organic Matrixes (2003)

Sulfur oxidation appears a very promising route for obtaining ultralow-sulfur fuels requested worldwide by the new regulation mandates. In this work, the oxidizing system constituted by hydrogen peroxide and formic acid was used to study the influence of the solvent on the oxidation rate of sulfur compounds in the organic phase. The experimentation was performed on organic sulfur compounds selected as representative of those contained in crude distillates. The organic solvent seems to play an important role in the reaction kinetics, indicating a strong influence of its aromaticity on the oxidation rate. A different kinetic was found for heterocyclic sulfur compounds such as benzo- and dibenzothiophene when compared with thiols and sulfides, indicating for the last ones a more complex reaction path.
Calculating the Surface Seismic Signal from a Trapped Miner (2012)

The recent Sago mine disaster has highlighted the need to fundamentally analyze the practical detection limitations of a mine rescue seismic system (Heasley, 2006). In various field tests, it was discovered that the surface peak particle velocity resulting from the trapped miner who pounds on the mine roof becomes undetectable past a certain distance from the surface geophones. In order to provide the mine rescuer the understanding of the exact extent of this distance; and the foreknowledge about the excepted magnitude of the surface peak particle movement in terms of: depth, geology and offset angle, this study was performed.;To achieve this goal, the following three major components were investigated: 1) the roof displacement resulting from the momentum of the impact of the signaling device when it hits the roof, 2) the pounding factor that relates the pounding instruments (sledgehammer or crib block) to the frequency and amplitude of the seismic signal, and 3) the attenuation of the displacement pulse as it moves through the overburden to the surface. To obtain these values, a parallel study of theoretical and experimental work was done (because of the uncertainties still associated with the mechanics of the roof momentum). In the theoretical analysis, a numerical model of the expected seismic attenuation of a given mine site was developed using an EXCEL spreadsheet. This model uses mine specific geology with the appropriate seismic parameters and vertical and horizontal offset distances to determine the expected attenuation. For the estimate of the pounding factor, the numerical modeling of the 4-West Mine where a field test took place was done to determine the seismic attenuation of the mine; and then the peak particle movements that were recorded during the seismic field experiment were back-analyzed to estimate the pounding factor.;With this simplified numerical analysis of the seismic attenuation and the representative' values for the pounding device pounding factor; the mine rescuer will be able to determine: 1) the expected magnitude of the surface peak particle movement, 2) the limiting distance where the signal would likely become undetectable, and 3) the chance of detecting a miner's signal with respect to the background noise level, and the appropriate steps to follow (either to implements appropriate procedures in order to reduce the noise level or to redirect the rescue efforts). It is believed that the information itemized above will be very useful to facilitate the optimization of the future mine rescue seismic
Configurable and networked web-based vertical applications (2002)

As network and mobile computing technologies evolving, the enterprise applications are also expanded to web-based platforms facilitated by these enabling technologies. The progress is further propelled by large-scale e-commerce activities such as B2C (business-to-consume) and B2B (business-to-business) applications. This paper proposes the use of observations and models of brain development in the early childhood for e-commerce and WWW growth models, particularly for B2C and B2B activities. Biological models enlighten the effectiveness of business cluster models. Based on these models, we propose a configurable and networked architecture and present a cost-effective implementation for supporting business collaboration by emerging Internet frameworks and AI technologies such as rule-based systems and neural networks.
ChemInform Abstract: Chemical RNA Modifications for Studies of RNA Structure and Dynamics (2010)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Long and entangled native cellulose I nanofibers allow flexible aerogels and hierarchically porous templates for functionalities (2008)

Recently it was shown that enzymatic and mechanical processing of macroscopic cellulose fibers lead to disintegration of long and entangled native cellulose I nanofibers in order to form mechanically strong aqueous gels (Pääkkö et al., Biomacromolecules, 2007, 8, 1934). Here we demonstrate that (1) such aqueous nanofibrillar gels are unexpectedly robust to allow formation of highly porous aerogels by direct water removal by freeze-drying, (2) they are flexible, unlike most aerogels that suffer from brittleness, and (3) they allow flexible hierarchically porous templates for functionalities, e.g. for electrical conductivity. No crosslinking, solvent exchange nor supercritical drying are required to suppress the collapse during the aerogel preparation, unlike in typical aerogel preparations. The aerogels show a high porosity of ∼98% and a very low density of ca. 0.02 g cm−3. The flexibility of the aerogels manifests as a particularly high compressive strain of ca. 70%. In addition, the structure of the aerogels can be tuned from nanofibrillar to sheet-like skeletons with hierarchical micro- and nanoscale morphology and porosity by modifying the freeze-drying conditions. The porous flexible aerogel scaffold opens new possibilities for templating organic and inorganic matter for various functionalities. This is demonstrated here by dipping the aerogels in an electrically conducting polyaniline–surfactant solution which after rinsing off the unbound conducting polymer and drying leads to electrically conducting flexible aerogels with relatively high conductivity of around 1 × 10−2 S cm−1. More generally, we foresee a wide variety of functional applications for highly porous flexible biomatter aerogels, such as for selective delivery/separation, tissue-engineering, nanocomposites upon impregnation by polymers, and other medical and pharmaceutical applications.
ASETS - An Academic Trading Simulation Platform (2010)

This paper is intended to present the results of our academic research upon a distributed computing environment dedicated to trading simulation. Our research has been conducted with the aim of creating a trading simulation platform, that would provide both the foundation for future experiments with trading systems architectures, components, APIs, and the framework for research on trading strategies, trading algorithms design, and equity markets analysis tools.