gebhart/patrepeval huggingface dataset:
Isolation and characterization of a 125 kDa rapidly labeled nucleolar phosphoprotein (1985)

A 125-kilodalton (kDa) phosphoprotein was isolated from nucleoli of Novikoff hepatoma cells in the presence of various inhibitors of proteases, alkaline phosphatase, and RNase. This protein was the most highly phosphorylated protein found thus far in the nucleolus. The half-life of [32P]phosphate in the 125-kDa phosphoprotein was approximately 60 min. Amino acid analysis of the protein showed it had a high serine content (15.5 mol %), a high glutamine plus glutamic acid content (15.5 mol %), and a high lysine content (10.3 mol %). Phosphoserine was the only phosphorylated amino acid identified. After alkaline hydrolysis of the 32P-labeled protein, ribonucleotides were found which accounted for approximately 8.5% of the [32P]phosphate. After cytidine 3',5'-[32P]diphosphate ([32P]pCp) labeling by RNA ligase, several oligoribonucleotide sequences were purified including GGGCOH and GGGGCOH. The binding of oligonucleotides to peptides was stable under denaturing fractionation conditions including 6 M urea treatment and incubation at 100 degrees C for 10 min in sodium dodecyl sulfate and beta-mercaptoethanol. Furthermore, when nucleotide-peptide complex was treated with ribonuclease T2 followed by snake venom phosphodiesterase, the junctional nucleotide pCp was released. These results suggest that one or more ribonucleotides are covalently bound to the 125-kDa phosphoprotein.
Hybridized Nanowires and Cubes: A Novel Architecture of a Heterojunctioned TiO<sub>2</sub>/SrTiO<sub>3</sub> Thin Film for Efficient Water Splitting (2010)

Abstract A unique morphology of SrTiO 3 nanocubes precipitated on TiO 2 nanowires is successfully synthesized in the form of a thin‐film heterojunctioned TiO 2 /SrTiO 3 photocatalyst using facile hydrothermal techniques. The formation mechanisms of the synthesized photocatalysts are meticulously studied and described. Growth of SrTiO 3 single crystal nanocubes (≈50 nm in width) on anatase polycrystalline nanowires follows an in situ dissolution‐precipitation pathway. This is consonant with the classic LaMer model. By analyzing the results of field emission scanning electron microscopy (FESEM), field emission transmission electron microscopy (FETEM), X‐ray diffraction (XRD), energy dispersive X‐ray (EDX) spectroscopy, X‐ray photoelectron spectroscopy (XPS), and UV‐vis spectrophotometry, a comprehensive structural and morphological characterization of the photocatalysts is established. FESEM images reveal that the anatase film comprises mainly of nanowires bristles while the tausonite film is primarily made up of nanocube aggregations. In comparison to the respective pristine semiconductor photocatalysts, the heterostructured photocatalyst demonstrates the highest efficiency in photocatalytic splitting of water to produce H 2 , 4.9 times that of TiO 2 and 2.1 times that of SrTiO 3 . The enhanced photocatalytic efficiency is largely attributed to the efficient separation of photogenerated charges at heterojunctions of the two dissimilar semiconductors, as well as a negative redox potential shift in the Fermi level.
Impact of Discrete Raman Amplifier Architecture on Nonlinear Impairments (2002)

We compare the nonlinear pulse distortion of single and dual-stage discrete Raman amplifiers and confirm reduced nonlinearity for the single-stage design in an all-Raman WDM transmission experiment in the S-band over 12×l00 km.
Induction of interleukin 1 beta expression from human peripheral blood monocyte-derived macrophages by 9-hydroxyoctadecadienoic acid. (1992)

Oxidatively modified low density lipoproteins (LDL) have recently been proposed to play a role in atherogenesis by promoting foam cell formation and endothelial cell toxicity. The purpose of the present study was to determine whether modified LDL could also induce macrophage release of interleukin 1 beta (IL-1 beta), a cytokine which enhances vascular smooth muscle cell proliferation, another feature of the atherosclerotic process. LDL were oxidatively modified by incubation with either Cu2+ (Cu(2+)-LDL) or human peripheral blood monocyte-derived macrophages (M-LDL). Incubation of these modified LDL with macrophages (6 x 10(6) cells/culture) resulted in a dose-dependent induction of IL-1 beta release. At 300 micrograms protein/ml, Cu(2+)-LDL and M-LDL induced 422 and 333 pg of IL-1 beta/culture, respectively. Saponified Cu(2+)-LDL and M-LDL were shown to contain 9- and 13-hydroxyoctadecadienoic acid (HODE), lipid oxidation products of linoleate. When tested for activity in macrophage culture (3 x 10(6) cells/culture), it was found that 9-HODE and 13-HODE (final concentration 33 microM) induced the release of 122 and 43 pg of IL-1 beta/culture, respectively, whereas untreated cells released only 4 pg of IL-1 beta/culture. Incubation of macrophages with cholesteryl-9-HODE also induced IL-1 beta release; however, the degree of induction of IL-1 beta release by 9-HODE or its cholesteryl ester relative to modified LDL suggests that other components in oxidized LDL may also contribute to IL-1 beta induction. 9-HODE was rapidly taken up by macrophages, and the kinetics were similar to IL-1 beta release. A 1.5- to 6-fold increase in the level of IL-1 beta mRNA was detected as little as 3-h post-9-HODE treatment. The induction of IL-1 beta release from human monocyte-derived macrophages by 9-HODE and cholesteryl-9-HODE suggests a role for modified LDL, and its associated linoleate oxidation products, in vascular smooth muscle cell proliferation.
Influence of polymer structural parameters and experimental conditions on metal anion sorption by chitosan (1999)

Chitosan is effective at removing molybdate from acidic solutions, especially when it is stabilized by crosslinking treatment. The influence of structural and physicochemical properties of chitosan and the glutaraldehyde crosslinking step on molybdate has been studied. This study shows that the maximum sorption capacity mainly depends on the crystallinity and degree of deacetylation. Hydration and accessibility to internal sites are controlled by these parameters, which may explain the intraparticle diffusion control on mass transfer. Chitosan gel beads can be used instead of flaked particles to decrease sorbent crystallinity. These sorbents were used in column systems, and the breakthrough curves obtained under several experimental conditions (flow velocity and column depth) were modelled using numerical analysis. Molybdate-impregnated chitosan beads (MICB) can be used for As(V) removal: sorption isotherms show high sorption levels even at low arsenic concentrations, and at pH 2–3, arsenic removal is optimal and molybdenum release is minimal. The desorption and regeneration of the sorbent was effective using phosphoric acid solutions. © 1999 Society of Chemical Industry
Improving NAND flash read performance through learning (2015)

Two important performance metrics for a storage system are the latency associated with retrieving data from its storage medium and the effective lifetime of its storage medium. Both metrics are directly affected by the number of raw read errors (i.e. errors prior to exploiting error-correction mechanisms). This paper focuses on NAND flash memories, where a read is performed by comparing stored voltages with a threshold voltage. The unwanted variation of stored voltages causes read errors. This paper identifies number of flash program-erase (PE) cycles, time elapsed between writing and reading, and the page number (physical location) as the main sources of voltage variations. It then proposes a method for learning how read thresholds should vary with these parameters such that the storage controller can dynamically vary thresholds and minimize read errors. Lab experiments show that at the flash end-of-life, the proposed method lowers the raw bit-error-rate up to a factor of 6, as compared to manufacturer's default read settings.
L’art et l’institution. Variations sur un même thème (2007)

peer reviewed
Transient Behavior and Analysis of Non-Darcy Flow in Porous and Fractured Reservoirs According to the Barree and Conway Model (2010)

Abstract This study introduces the usage of the Barree and Conway flow model in well testing pressure-transient applications for a single-phase non-Darcy flow in porous and fractured reservoirs. The non-Darcy flow behavior is handled using a three-dimensional, general purpose reservoir simulator. This numerical model incorporates non-Darcy flow effect according to the Barree and Conway flow model and the developed numerical model is capable of simulating all near wellbore effects, such as the wellbore storage and skin effects, coupled with the non-Darcy flow behavior. In addition, a steady-state non-Darcy radial flow solution is derived analytically according to the Barree and Conway model. The numerical simulation results are verified with the analytical solution. The numerical model is used to model and interpret the radial flow pressure-transient responses for both pressure buildup and drawdown well tests in porous and fractured reservoirs. In simulating pressure drawdown tests with the non-Darcy flow effects according to Barree and Conway model and assuming no skin and wellbore storage effects, the permeability, estimated using the standard straight-line analysis, is an apparent permeability and not the Darcy's constant permeability. The estimated permeability ranges from the minimum permeability and to less than the Darcy's permeability. Thus in pressure drawdown tests the standard straight-line analysis techniques underestimate the Darcy's permeability when non-Darcy flow behavior exists. A pressure buildup test, following non-Darcy flow drawdown tests, may be good for determining Darcy's permeability values using standard straight-line analysis when non-Darcy flow effect is insignificant. The Barree and Conway non-Darcy flow model parameters may not be directly estimated from the pressure-transient well tests with a simple graphic approach. However, they can be estimated by a matching process, based on non-linear optimization algorithm incorporated into the developed numerical model. The type curves generated by the numerical model are provided to demonstrate a methodology for modeling single phase transient non-Darcy flow behavior in porous and fractured rocks. The developed numerical model in this study is used to interpret and match actual pressure drawdown and buildup well tests from wells of high production rates in Kuwait.
A comparative study of medetomidine/ketamine and xylazine/ketamine anaesthesia in dogs. (1990)

The anaesthetic and physiological effects of a combination of 40 micrograms medetomidine with 2.5 ketamine, 5.0 or 7.5 mg/kg administered intramuscularly were compared with the effects of a combination of 1 mg/kg xylazine and 15 mg/kg ketamine. All the combinations rapidly induced an anaesthetic state that permitted endotracheal intubation, with the absence of the pedal reflex and with good muscle relaxation, and induced bradycardia that was less pronounced as the dose of ketamine was increased. All the combinations produced a decrease in respiratory rate. Increasing the dose of ketamine combined with medetomidine resulted in a very significant prolongation of the duration of anaesthesia, the duration of muscle relaxation and the arousal time. The duration of the anaesthetic effects of 40 micrograms/kg medetomidine with 5 mg/kg ketamine was comparable to that provided by the recommended xylazine/ketamine combination but the period of muscle relaxation was significantly longer. The recovery from medetomidine/ketamine took longer than recovery from xylazine/ketamine but there were fewer side effects.
Japan's Role in the Asian Environmental Crisis: Comparingthe Critical Literature and the Environmental Agency's White Papers (2001)

The late 1980s saw the development of two bodies of literature addressing Japan’s relationship to the Asian environmental crisis.The Japanese government’s statements were concerned primarily with the contributions Japan could make to solving that crisis, while critical scholars and NGOs focused on ways in which Japanese activity exacerbated it. Using the annual White Papers of the Japan Environment Agency as a guide, this survey article inquires into the extent to which the government has come to engage with the arguments of the critical literature during the 1990s, a decade in which governments and multilateral lending agencies have been forced to deal more closely with environmental concerns. In particular I discuss (1) the extent to which the white papers address Japan’s negative impacts on Asia; (2) the ways they treat Japan as a model for Asian environmental policy; and (3) their approach to conflict over policy-making. Protecting the global environment became a major theme of Japanese diplomacy at the turn of the 1990s. Prompted by growing international concern over global environmental problems and the opportunity to use these new issues for political benefit, a number of actors within the LDP and the bureaucracy began to stress Japan’s potential contribution to solving problems such as global warming (Schreurs 1997). Japan’s combination of deep pockets and abundant experience with antipollution technology were presented in government rhetoric as giving the country both the ability and the responsibility to tackle these issues (Japan Environment Agency (JEA) 1990; Government of Japan 1991). It was also argued that Japan’s close relationship with Asia meant that its efforts at environmental protection should be concentrated there. The years before and after the 1992 United Nations Conference on Environment and Development (UNCED) thus saw the announcement of a series of expensive new environmental initiatives in research and development, education and Official Development Assistance (ODA).
Verification oriented development of a scalable battery management system for lithium-ion batteries (2017)

This paper describes the model-based development and validation of a flexible scalable battery management system (BMS) for lithium-ion batteries (LiFePO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> ) using a verification-oriented development methodology. The BMS consists of a central control unit and decentral cell modules. It measures voltages, currents and temperatures to ensure safe and slow aging operating states. Furthermore, appropriate algorithms estimate the state of charge and predict the maximum battery power in a defined prediction horizon Δt, based on the measurement data. The entire capacity of the production-related slightly differing battery cells can be optimally used by using passive or active load balancing methods which equalize the cell charging levels. The BMS also has a CAN interface for integration into a vehicle ECU network. The development of an Extended Kalman Filter (EKF) as a state of charge (SOC) estimator will be shown as an example for the model-based design process.
Molecular imaging. A new approach to nuclear cardiology. (2005)

Nuclear cardiology has historically played an important role in detection of cardiovascular disease as well as risk stratification. With the growth of molecular biology have come new therapeutic interventions and the requirement for new diagnostic imaging approaches. Noninvasive targeted radiotracer based as well as transporter gene imaging strategies are evolving to meet these new needs, but require the development of an interdisciplinary approach which focuses on molecular processes, as well as the pathogenesis and progression of disease. This progress has been made possible with the availability of transgenic animal models along with many technological advances. Future adaptations of the developing experimental procedures and instrumentation will allow for the smooth translation and application to clinical practice. This review is intended as a brief overview on the subject molecular imaging. Basic concepts and historical perspective of molecular imaging will be reviewed first, followed by description of current technology, and concluding with current applications in cardiology. The emphasis will be on the use of both single photon emission computed tomography (SPECT) and positron emission tomography (PET) radiotracers, although other imaging modalities will be also briefly discussed. The specific approaches presented here will include receptor-based and reporter gene imaging of natural and therapeutic angiogenesis.
Effect of pH and Ionic Strength on the Physicochemical Properties of Coconut Milk Emulsions (2008)

Coconut milk (16% to 17% fat, 1.8% to 2% protein) was extracted from coconut (Cocos nucifera L.) endosperm and diluted in buffer to produce natural oil-in-water emulsions (10 wt% oil). The effect of pH (3 to 7) and NaCl (0 to 200 mM) on the properties and stability, namely, mean particle size, zeta-potential, viscosity, microstructure, and creaming stability, of the natural coconut milk emulsions was investigated. At pH values close to the isoelectric point (IEP) of the coconut proteins (pH 3.5 to 4) and in the absence of NaCl, coconut milk flocculated, but did not coalesce. Flocculation corresponded to low surface charges and was accompanied by an increase in emulsion viscosity. Adding up to 200 mM NaCl to those flocculated emulsions did not change the apparent degree of flocculation. Coconut milk emulsion at pH 6 was negatively charged and not flocculated. Upon addition of salt, the zeta-potential decreased from -16 to -6 mV (at 200 mM NaCl) but this was not sufficient to induce flocculation in coconut milk emulsions. At low pH (< IEP), the positively charged droplets of coconut milk emulsions only flocculated when the NaCI concentration exceeded 50 mM, as the zeta-potential approached zero.
Quantifying 3-D Vascular Structures in MRA Images Using Hybrid PDE and Geometric Deformable Models (2004)

The aim of this paper is to present a hybrid approach to accurate quantification of vascular structures from magnetic resonance angiography (MRA) images using level set methods and deformable geometric models constructed with 3-D Delaunay triangulation. Multiple scale filtering based on the analysis of local intensity structure using the Hessian matrix is used to effectively enhance vessel structures with various diameters. The level set method is then applied to automatically segment vessels enhanced by the filtering with a speed function derived from enhanced MRA images. Since the goal of this paper is to obtain highly accurate vessel borders, suitable for use in fluid flow simulations, in a subsequent step, the vessel surface determined by the level set method is triangulated using 3-D Delaunay triangulation and the resulting surface is used as a parametric deformable model. Energy minimization is then performed within a variational setting with a first-order internal energy; the external energy is derived from 3-D image gradients. Using the proposed method, vessels are accurately segmented from MRA data.
Normalizing DNA Microarray Data (2002)

DNA microarrays are a powerful tool to investigate differential gene expression for thousands of genes simultaneously. Although DNA microarrays have been widely used to understand the critical events underlying growth, development, homeostasis, behavior and the onset of disease, the management of the resulting data has received little attention. Presently, the fluorescent dyes Cy3 and Cy5 are most often used to prepare labeled cDNA for microarray hybridizations. Raw microarray data are image files that have to be transformed into gene expression formats--a process that requires data manipulation due to systematic variations which may be attributed to differences in the physical and chemical dye applications is to identify differences in transcript levels calculated from fluorescence ratios it is necessary to normalize fluorescence signals to compensate for systematic variations. Here, we will review current normalization strategies applied to cDNA microarrays and discuss their limits. We will show that experimental design determines normalization success.
New device for saphenous vein-to-aorta proximal anastomosis without side-clamping (2007)

The object was named "Slit Device" and is not a routine instrument. It would be only an alternative to other anastomotic devices with the same surgical indications. In the case of ascending aortic disease and saphenous vein grafting, the Slit Device avoids aortic clamping thereby preventing atheroembolism and also avoiding the need for hypothermic circulatory arrest in patients with unclampable aorta.
Proteomics with Mass Spectrometry (2011)

This chapter provides a rudimentary review of the field of proteomics as it applies to mass spectrometry, data handling, and analysis. It points out the potential significance of the field suggesting that the study of nuclei acids has its limitations and that the progressive field of proteomics with spectrometry in tandem with transcription studies could potentially elucidate the link between RNA transcription and concomitant protein expression. Furthermore, we describe the fundamentals of proteomics with mass spectrometry and expound the methodology necessary to manage the vast amounts of data generated in order to facilitate statistical analysis. We explore the underlying technologies with the intention to demystify the complexities of the nascent field and to fuel interest by readers in the near future.Request access from your librarian to read this chapter's full text.
Synthesis of Antimalarials. VI.<sup>1</sup> Synthesis of Certain 1,5- and 1,8-Naphthyridine Derivatives<sup>2</sup> (1946)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of Antimalarials. VI.1 Synthesis of Certain 1,5- and 1,8-Naphthyridine Derivatives2Joe T. Adams, Charles K. Bradsher, David S. Breslow, S. Thomas Amore, and Charles R. HauserCite this: J. Am. Chem. Soc. 1946, 68, 7, 1317–1319Publication Date (Print):July 1, 1946Publication History Published online1 May 2002Published inissue 1 July 1946https://pubs.acs.org/doi/10.1021/ja01211a064https://doi.org/10.1021/ja01211a064research-articleACS PublicationsRequest reuse permissionsArticle Views462Altmetric-Citations36LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Randomized Assessment of the Safety and Efficacy of Intra-Arterial Infusion of Autologous Stem Cells in Subacute Ischemic Stroke (2018)

Intra-arterial infusion of stem cells can be carried out safely in the subacute stage of ischemic stroke. Improved clinical outcomes were observed with intra-arterial stem cell therapy; however, studies with larger cohorts are needed to validate the results.