gebhart/patrepeval huggingface dataset:
A Smart-Home System to Unobtrusively and Continuously Assess Loneliness in Older Adults (2016)

Loneliness is a common condition in older adults and is associated with increased morbidity and mortality, decreased sleep quality, and increased risk of cognitive decline. Assessing loneliness in older adults is challenging due to the negative desirability biases associated with being lonely. Thus, it is necessary to develop more objective techniques to assess loneliness in older adults. In this paper, we describe a system to measure loneliness by assessing in-home behavior using wireless motion and contact sensors, phone monitors, and computer software as well as algorithms developed to assess key behaviors of interest. We then present results showing the accuracy of the system in detecting loneliness in a longitudinal study of 16 older adults who agreed to have the sensor platform installed in their own homes for up to 8 months. We show that loneliness is significantly associated with both time out-of-home (β = -0.88 andp <; 0.01) and number of computer sessions (β = 0.78 and p <; 0.05). R <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> for the model was 0.35. We also show the model's ability to predict out-of-sample loneliness, demonstrating that the correlation between true loneliness and predicted out-of-sample loneliness is 0.48. When compared with the University of California at Los Angeles loneliness score, the normalized mean absolute error of the predicted loneliness scores was 0.81 and the normalized root mean squared error was 0.91. These results represent first steps toward an unobtrusive, objective method for the prediction of loneliness among older adults, and mark the first time multiple objective behavioral measures that have been related to this key health outcome.
Variations on loop and Adcock array topologies for improved radio direction finding performance (2012)

We present what we believe to be two novel and related antenna topologies that find application in radio direction finding (DF). A topology for a stabilised anti-phase dual-fed loop antenna is presented that reduces sensitivity to cross-polarised wave incidence, increases loop gain at higher frequencies and provides improved pattern stability when compared to traditional loop antenna designs. Additionally, we present a hybrid Adcock/loop topology that provides the desirable qualities of Adcock arrays at high frequencies whilst substantially improving the low frequency sensitivity of electrically small Adcock arrays. Design and measurements are presented for both variations.
Human major histocompatibility complex class II‐negative colon carcinoma cells present staphylococcal superantigens to cytotoxic T lymphocytes: evidence for a novel enterotoxin receptor (1991)

The staphylococcal enterotoxins (SE) bind to major histocompatibility complex (MHC) class II molecules on target cells and activate T cells expressing particular T cell receptor V beta sequences. In this report we demonstrate that SE bind to the MHC class II- SW620, Colo320DM and WiDr human colon carcinoma cell lines and direct cytotoxic T lymphocytes (CTL) to mediate strong target cell killing. Flow cytometry analysis, immunoprecipitation and Northern blotting experiments failed to demonstrate any surface expression of HLA-DR, HLA-DP and HLA-DQ isotypes on the SW620 colon carcinoma cell line, whereas abundant expression of these isotypes was seen on Raji cells, SEB and SEC1 were efficiently presented at picomolar concentration by the MHC class II- colon carcinoma cells and MHC class II+ Raji cells, whereas SEA and SED were preferentially presented on the MHC class II+ Raji cells. An anti-HLA-DR monoclonal antibody inhibited SEB-induced CTL targeting to Raji, but did not influence the killing of SW620 cells. Our data suggests the existence of functionally active SE-binding structures on human colon carcinoma cells which are distinct from the conventional MHC class II molecules. The possibility that these putative new SE receptors play a role in the enterotoxin action of SE must be considered.
Chk1 Targeting Reactivates PP2A Tumor Suppressor Activity in Cancer Cells (2013)

Abstract Checkpoint kinase Chk1 is constitutively active in many cancer cell types and new generation Chk1 inhibitors show marked antitumor activity as single agents. Here we present a hitherto unrecognized mechanism that contributes to the response of cancer cells to Chk1-targeted therapy. Inhibiting chronic Chk1 activity in cancer cells induced the tumor suppressor activity of protein phosphatase protein phosphatase 2A (PP2A), which by dephosphorylating MYC serine 62, inhibited MYC activity and impaired cancer cell survival. Mechanistic investigations revealed that Chk1 inhibition activated PP2A by decreasing the transcription of cancerous inhibitor of PP2A (CIP2A), a chief inhibitor of PP2A activity. Inhibition of cancer cell clonogenicity by Chk1 inhibition could be rescued in vitro either by exogenous expression of CIP2A or by blocking the CIP2A-regulated PP2A complex. Chk1-mediated CIP2A regulation was extended in tumor models dependent on either Chk1 or CIP2A. The clinical relevance of CIP2A as a Chk1 effector protein was validated in several human cancer types, including neuroblastoma, where CIP2A was identified as an NMYC-independent prognostic factor. Because the Chk1–CIP2A–PP2A pathway is driven by DNA-PK activity, functioning regardless of p53 or ATM/ATR status, our results offer explanative power for understanding how Chk1 inhibitors mediate single-agent anticancer efficacy. Furthermore, they define CIP2A-PP2A status in cancer cells as a pharmacodynamic marker for their response to Chk1-targeted therapy. Cancer Res; 73(22); 6757–69. ©2013 AACR.
Selection of aptamers for signal transduction proteins by capillary electrophoresis (2010)

High-affinity aptamers for important signal transduction proteins, i.e. Cdc42-GTP, p21-activated kinase1 (PAK1) and MRCK (myotonic dystrophy kinase-related Cdc42-binding kinase) alpha were successfully selected in the low micro- to nanomolar range using non-systematic evolution of ligands by exponential enrichment (SELEX) with at least three orders of magnitude enhancement from their respective bulk affinity of naïve DNA library. In the non-SELEX procedure, CE was used as a highly efficient affinity method to select aptamers for the desired molecular target through a process that involved repetitive steps of partitioning, known as non-equilibrium CE of equilibrium mixtures with no PCR amplification between successive steps. Various non-SELEX conditions including the type, concentration and pH of the run buffer were optimized. Other considerations such as salt composition of selection buffer, protein concentration and sample injection size were also studied for high stringency during selection. After identifying the best enriched aptamer pool, randomly selected clones from the aptamer pool were sequenced to obtain the individual DNA sequences. The dissociation constants (K(d)) of these sequences were in the low micromolar to nanomolar range, indicating high affinity to the respective proteins. The best binders were also subjected to sequence alignment to generate a phylogenetic tree. No significant consensus region based on approximately 50 sequences for each protein was observed, suggesting the high efficiency of non-SELEX for the selection of numerous unique sequences with high selectivity.
Ion selective electrodes, potentiometry, and potentiometric titrations (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIon selective electrodes, potentiometry, and potentiometric titrationsRichard P. BuckCite this: Anal. Chem. 1974, 46, 5, 28–51Publication Date (Print):April 1, 1974Publication History Published online1 May 2002Published inissue 1 April 1974https://pubs.acs.org/doi/10.1021/ac60341a020https://doi.org/10.1021/ac60341a020research-articleACS PublicationsRequest reuse permissionsArticle Views998Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Systèmes aromatiques à 10 électrons π dérivés de l'aza‐3a pentalène. XXXII. Recherches dans la série du pyrazolo[1,5‐a]benzimidazole (1978)

Abstract Le probleme de l'aromatiuité d'un dérivé aza‐3a pentalénique, le méthyl‐2 pyrazolo[1,5‐a]‐benzimidazole, a été abordé par 1′étude de sa réactivité comparée à celle d'un produit modèle, le phényl‐1 méthyl‐3 amino‐5 pyrazole. La mono‐ et diprotonation, l'alcoylation, la quater‐narisation, la formylation, l'acétylation et le comportement des dérivés N ‐acétylés ont été examinés. On eonclue à une structure aromatique fortement polarisée.
Investigation of spurious emissions from cellular phones and the possible effect on aircraft navigation equipment (2003)

The study described in this paper investigated the possible interference of spurious emissions from cellular phones on aircraft navigation and communication equipment. Six wireless phone technologies were investigated over three frequency ranges, which covered the receiving ranges of five aircraft systems. The aircraft systems examined were very-high frequency omnidirectional range (VOR), localizer (LOC), very high frequency communication (VHF), glide slope (GS), and global positioning system (GPS). Research was performed in a semi-anechoic chamber (SAC) and a reverberation chamber (RC), with the phone at a distance of 1 m from the receiving antenna. Two antenna orientations were tested in the SAC. Initial tests yielded the appropriate height from the receiving antenna above the ground plane that would produce a maximum field measurement. Keypad programming was used to simulate worst-case phone transmissions. Radiated power measurements were performed and analyzed taking into consideration path loss results from the RTCA/DO-233 study. None of the six phone technologies investigated exhibited a power level greater than a 38-dBm path loss level above the maximum sensitivity of the aircraft system antennae when tested 1 m away from the antenna. These results indicate that, for the aircraft systems tested (VOR, LOC, VHF, GS, and GPS), the antenna of these systems would not have detected the emissions of the phones used in this study (CDMA-cellular, TDMA-11 Hz, TDMA-50 Hz cellular and PCS, GSM, and DCS-1800).
A cluster-based approach for routing in dynamic networks (1997)

The design and analysis of routing protocols is an important issue in dynamic networks such as packet radio and ad-hoc wireless networks. Most conventional protocols exhibit their least desirable behavior for highly dynamic interconnection topologies. We propose a new methodology for routing and topology information maintenance in dynamic networks. The basic idea behind the protocol is to divide the graph into a number of overlapping clusters. A change in the network topology corresponds to a change in cluster membership. We present algorithms for creation of clusters, as well as algorithms to maintain them in the presence of various network events. Compared to existing and conventional routing protocols, the proposed cluster-based approach incurs lower overhead during topology updates and also has quicker reconvergence. The effectiveness of this approach also lies in the fact that existing routing protocols can be directly applied to the network --- replacing the nodes by clusters.
1851 (2005)

Freilich stehen in der Nachbildung der hebräischen Gesänge, namentlich derer, die nach arabischem Muster in künstlichen Versmaßen gedichtet sind, dem Uebersetzer fast unübersteigliche Hindernisse entgegen, und die Kürze und Gedrungenheit des Ausdrucks sowie der Reiz der eingelegten Bibelverse ist nicht wieder zu geben, so daß der des Hebräischen Unkundige nicht leicht einen Begriff von der Schönheit und dem Zauber des Originals empfängt.
THE DNA REPLICATION FORK IN EUKARYOTIC CELLS (1998)

Replication of the two template strands at eukaryotic cell DNA replication forks is a highly coordinated process that ensures accurate and efficient genome duplication. Biochemical studies, principally of plasmid DNAs containing the Simian Virus 40 origin of DNA replication, and yeast genetic studies have uncovered the fundamental mechanisms of replication fork progression. At least two different DNA polymerases, a single-stranded DNA-binding protein, a clamp-loading complex, and a polymerase clamp combine to replicate DNA. Okazaki fragment synthesis involves a DNA polymerase-switching mechanism, and maturation occurs by the recruitment of specific nucleases, a helicase, and a ligase. The process of DNA replication is also coupled to cell-cycle progression and to DNA repair to maintain genome integrity.
Exploring protein orthogonality in immune space: a case study with AAV and Cas9 orthologs (2018)

ABSTRACT A major hurdle in protein-based therapeutics is the interaction with the adaptive immune system, which can lead to neutralization by circulating antibodies and clearance of treated cells by cytotoxic T-lymphocytes. One method of circumventing these issues is to use human or humanized proteins which avoid the immune response by self-recognition. However, this approach limits potential protein therapeutics to those of human origin, excluding many exciting effectors and delivery vehicles such as CRISPR-Cas9 and adeno-associated viruses (AAVs). To address this issue, we propose here the sequential use of orthologous proteins whose function is constrained by natural selection, but whose structure is subject to diversification by genetic drift. This would, in principle, allow for repeated treatments by ‘immune orthogonal’ orthologs without reduced efficacy due to lack of immune cross-reactivity among the proteins. To explore and validate this concept we chose 91 Type II CRISPR-Cas9 orthologs and 167 AAV capsid protein orthologs, and developed a pipeline to compare total sequence similarity as well as predicted binding to class I and class II Major Histocompatibility Complex (MHC) proteins. Interestingly, MHC binding predictions revealed wide diversity among the set of Cas9 orthologs, with 83% of pairs predicted to have non cross-reacting immune responses, while no global immune orthogonality among AAV serotypes was observed. To confirm these findings we selected two Cas9 orthologs, from S. pyogenes and S. aureus , predicted to be orthogonal in immune space, and delivered them into mice via multiple AAV serotypes. We observed cross-reacting antibodies against AAV but not Cas9 orthologs in sera from immunized mice, validating the computationally predicted immune orthogonality among these proteins. Moving forward, we anticipate this framework can be applied to prescribe sequential regimens of immune orthogonal protein therapeutics to circumvent pre-existing or induced immunity, and eventually, to rationally engineer immune orthogonality among protein orthologs.
<i>In vitro</i>studies on the evaluation of mycotoxin detoxifying agents for their efficacy on deoxynivalenol and zearalenone (2004)

A simple in vitro system was developed to study the efficacy of commercially available mycotoxin detoxifying agents and adsorbing substances as feed additives to detoxify deoxynivalenol (DON) and zearalenone (ZON) in situ. The in vitro model simulates the conditions (pH, temperature and transit time) of the porcine gastrointestinal tract, as pigs react most sensitively to these mycotoxins. The commercially available products were not effective in detoxifying DON and ZON under the applied conditions, while activated carbon was able to bind both toxins and cholestyramine, and a modified aluminosilicate showed good adsorption abilities for ZON. Data obtained in dose dependency studies showed an estimated adsorption capacity of cholestyramine and the modified aluminosilicate of 11.7 and 5.7 g ZON/kg detoxifying agent. The in vitro system deployed in the present study was demonstrated to be a simple, helpful tool in screening substances for their ability to detoxify DON and ZON under the simulated conditions of the porcine gastrointestinal tract. Nonetheless in vivo experiments are indispensable to proof the efficacy.
Experimental Assessment of the Backoff Behavior of Commercial IEEE 802.11b Network Cards (2007)

It has been observed that different IEEE 802.11 commercial cards produced by different vendors experience different performance, either when accessing alone the channel, as well as when competing against each other. These differences persist also when thorough measurement methodologies (such as RF shielding, laptop rotation, etc) are applied, and alignment of the environmental factors (same laptop models, traffic generators, etc) is carried out. This paper provides an extensive experimental characterization of the backoff operation of six commercial NIC cards. It suggests a relevant methodological approach, namely a repeatable, well defined, set of experiments, for such a characterization. Low level backoff distribution measurements are taken through a custom equipment developed in our laboratory. Our work allows to detect both a non-standard backoff behavior of some commercial cards (in terms of minimum contention window size and neglection of EIFS times), as well as potential implementation limits (in either the card hardware/firmware and/or the software driver) which appear to severely alter the card performance in challenging conditions.
Sensitivity to Change and the Effect of Mode of Administration on Health Status Measurement (1987)

A measure of global health status, the McMaster Health Index Questionnaire (MHIQ), was assessed to determine two important measurement properties related to its reliability and validity--sensitivity to change and the effect of mode of administration. Ninety-six patients in a physiotherapy clinic were randomly assigned to three mode-of-administration study groups, administered the MHIQ at four points in time including at admission and discharge from the clinic, and assessed for change by their physiotherapist. MHIQ physical function, social function, and emotional function retest scores obtained by self-completion within a 1-week interval were most stable. Physical function scores by any of self-completion, telephone interview, or personal interview were sensitive to change, that is, they improved dramatically by the time of discharge from the clinic. Mode of administration did not affect the size of the change scores. Changes reflected by the physical function scores correlated with changes in physical function reported by a patient's physiotherapist. No systematic changes occurred with social and emotional function scores; this is not surprising in a group of patients with predominantly physical function problems.
RPFF: A Remote Page-Fault Filter for Post-copy Live Migration (2015)

Live migration of virtual machine has attracted significant attention in recent years. It facilitates system online maintenance, load balancing, fault tolerance and power management. Existing pre-copy live migration approach has to iteratively copy redundant memory pages, which causes high network overhead and slow migration. Another post-copy live migration approach can provide quick migration with low network overhead but would lead to a lot of remote page faults which greatly degrade application performance. In this paper, we improve the post-copy approach by eliminating unnecessary remote page faults. In our improved post-copy approach, page faults caused by guest's overwriting operations on remote memory pages are intercepted and handled by allocating new local memory pages, instead of fetching memory pages from the source host across the network. We have implemented our approach into the KVM/QEMU hypervisor and ran a series of experiments with Linux guests. The experimental results demonstrate that our improved approach performs much better than traditional post-copy approach.
SODIUM ZEOLITE ZK-4, A NEW SYNTHETIC CRYSTALLINE ALUMINOSILICATE (1961)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSODIUM ZEOLITE ZK-4, A NEW SYNTHETIC CRYSTALLINE ALUMINOSILICATEGeorge T. Kerr and George T. KokotailoCite this: J. Am. Chem. Soc. 1961, 83, 22, 4675Publication Date (Print):November 1, 1961Publication History Published online1 May 2002Published inissue 1 November 1961https://pubs.acs.org/doi/10.1021/ja01483a052https://doi.org/10.1021/ja01483a052research-articleACS PublicationsRequest reuse permissionsArticle Views422Altmetric-Citations65LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Link-based and content-based evidential information in a belief network model (2000)

This work presents an information retrieval model developed to deal with hyperlinked environments. The model is based on belief networks and provides a framework for combining information extracted from the content of the documents with information derived from cross-references among the documents. The information extracted from the content of the documents is based on statistics regarding the keywords in the collection and is one of the basis for traditional information retrieval (IR) ranking algorithms. The information derived from cross-references among the documents is based on link references in a hyperlinked environment and has received increased attention lately due to the success of the Web. We discuss a set of strategies for combining these two types of sources of evidential information and experiment with them using a reference collection extracted from the Web. The results show that this type of combination can improve the retrieval performance without requiring any extra information from the users at query time. In our experiments, the improvements reach up to 59% in terms of average precision figures.