gebhart/patrepeval huggingface dataset:
An acoustic wave biosensor for probing the viscoelastic properties of living cells (2006)

The thickness shear mode (TSM) resonator attached with living cells has been shown to be an effective functional biosensing device to monitor the process of cell adhesion to a surface. In this study, we first monitored the dynamic process of cell attachment and spreading as a function of cell seeding densities. Based on the steady state of cell adhesion to the substrate, a multilayer sensor structure model including a quartz substrate, a cell-substrate interfacial layer and a cell layer was constructed. The thickness of cell-substrate interfacial layer and the viscoelastic properties of human skin fibroblasts (HSF) were then determined by fitting experimental results with the theoretical model. It has been obtained that the thickness of the cell-substrate interfacial layer is 60-80 nm, and the elastic module and viscosity of cell layers are about 13 KPa and 3-4 mPa's respectively. These results are in a good agreement with those measured by other techniques, such as magnetic bead microrheometry, atomic force microscopy (AFM) and Surface Plasmon Resonance Microscopy (SPRM). In addition, knowing that the actin cytoskeleton is important for the mechanical properties of living cells, we investigated the motional resistance change caused by the disruption of actin cytoskeleton induced by fungal toxin Cytochalasin D in the human skin fibroblasts. The results indeed indicate the direct correlation between resistance changes and the disruption of actin cytoskeleton, which are again consistent with the results observed by fluorescence images.
PROSPECTIVE EXPLORATION OF BIOCHEMICAL TISSUE COMPOSITION VIA IMAGING MASS SPECTROMETRY GUIDED BY PRINCIPAL COMPONENT ANALYSIS (2006)

MALDI-based Imaging Mass Spectrometry (IMS) is an analytical technique that provides the opportunity to study the spatial distribution of biomolecules including proteins and peptides in organic tissue. IMS measures a large collection of mass spectra spread out over an organic tissue section and retains the absolute spatial location of these measurements for analysis and imaging. The classical approach to IMS imaging, producing univariate ion images, is not well suited as a first step in a prospective study where no a priori molecular target mass can be formulated. The main reasons for this are the size and the multivariate nature of IMS data. In this paper we describe the use of principal component analysis as a multivariate pre-analysis tool, to identify the major spatial and mass-related trends in the data and to guide further analysis downstream. First, a conceptual overview of principal component analysis for IMS is given. Then, we demonstrate the approach on an IMS data set collected from a transversal section of the spinal cord of a standard control rat.
La historiografía regional y local en América Latina y el Caribe (una visión desde Cuba) (1999)

Los estudios históricos regionales y locales con una óptica contemporánea han tenido en el último cuarto de siglo un notable aunque variado desarrollo a nivel internacional, pero en los últimos años han adquirido otras dimensiones que, muchas veces, han escapado de la acuciosa mirada de nuestros colegas. Estas dimensiones están directa, aunque no exclusivamente, entroncadas con los procesos de globalización, cuyos primeros ecos llegaron a nosotros desde la lejana región (¿macrorregión?) de Nagorni Karabaj, donde armenios y adzerbaizhanos dirimieron sus diferencias regionales, nacionales, culturales, religiosas y étnicas.
Modification of NR/BR blend with unsaturated thermo plastic polyurethane; polyurethane synthesis, blend morphology, vulcanization, and vulcanizate properties (2006)

Abstract The unsaturated polyurethane (PU) was synthesized, characterized, and used in rubber compound. To study the effect of PU incorporation in the blend a systematic approach was used where PU was added to the natural rubber (NR), butadiene rubber (BR), and NR/BR blends. Different un-vulcanized and vulcanized compounds were characterized and the vulcanization process was investigated. It was observed that the synthesized PU was able to crosslink with the rubber compounds during the vulcanization process. The lowered temperature of melting indicated a better processability of the composites containing thermoplastic PU which melted at processing temperature, thereby allowing improvement in blend viscosity mold flow and to some extent in its homogeneity, although the mechanical properties such as hardness, tensile strength, modulus, and elongation at break did not differ significantly from the properties of elastomer blends without PU. The thermal stability of compounds with PU did not significantly worsen.
Mycobacterium tuberculosis Type VII Secreted Effector EsxH Targets Host ESCRT to Impair Trafficking (2013)

Mycobacterium tuberculosis (Mtb) disrupts anti-microbial pathways of macrophages, cells that normally kill bacteria. Over 40 years ago, D'Arcy Hart showed that Mtb avoids delivery to lysosomes, but the molecular mechanisms that allow Mtb to elude lysosomal degradation are poorly understood. Specialized secretion systems are often used by bacterial pathogens to translocate effectors that target the host, and Mtb encodes type VII secretion systems (TSSSs) that enable mycobacteria to secrete proteins across their complex cell envelope; however, their cellular targets are unknown. Here, we describe a systematic strategy to identify bacterial virulence factors by looking for interactions between the Mtb secretome and host proteins using a high throughput, high stringency, yeast two-hybrid (Y2H) platform. Using this approach we identified an interaction between EsxH, which is secreted by the Esx-3 TSSS, and human hepatocyte growth factor-regulated tyrosine kinase substrate (Hgs/Hrs), a component of the endosomal sorting complex required for transport (ESCRT). ESCRT has a well-described role in directing proteins destined for lysosomal degradation into intraluminal vesicles (ILVs) of multivesicular bodies (MVBs), ensuring degradation of the sorted cargo upon MVB-lysosome fusion. Here, we show that ESCRT is required to deliver Mtb to the lysosome and to restrict intracellular bacterial growth. Further, EsxH, in complex with EsxG, disrupts ESCRT function and impairs phagosome maturation. Thus, we demonstrate a role for a TSSS and the host ESCRT machinery in one of the central features of tuberculosis pathogenesis.
Detection and sizing of particulate contamination in rigid disk drives: instruments and sampling techniques (1999)

Aerosol sampling instruments, their operating principles, availability, applicability with respect to head-disk interface (HDI) research and manufacturing, and price are presented. Experiments were conducted using a laser particle counter to address sampling issues of background elimination, sampling frequency and sampling location for HDI tests. Results show that continuous sampling was acceptable and sampling at either leading or trailing edge of the interface did not affect sampling performance appreciably.
Induced Pluripotent Stem Cells: Problems and Advantages when Applying them in Regenerative Medicine (2010)

Induced pluripotent stem cells (iPSCs) are a new type of pluripotent cells that can be obtained by reprogramming animal and human differentiated cells. In this review, issues related to the nature of iPSCs are discussed and different methods of iPSC production are described. We particularly focused on methods of iPSC production without the genetic modification of the cell genome and with means for increasing the iPSC production efficiency. The possibility and issues related to the safety of iPSC use in cell replacement therapy of human diseases and a study of new medicines are considered.
Cooperation among Wirelessly Connected Static and Mobile Sensor Nodes for Surveillance Applications (2013)

This paper presents a bio-inspired networking strategy to support the cooperation between static sensors on the ground and mobile sensors in the air to perform surveillance missions in large areas. The goal of the proposal is to provide low overhead in the communication among sensor nodes, while allocating the mobile sensors to perform sensing activities requested by the static ones. Simulations have shown that the strategy is efficient in maintaining low overhead and achieving the desired coordination.
Antineoplastic effects of melatonin on a rare malignancy of mesenchymal origin: melatonin receptor‐mediated inhibition of signal transduction, linoleic acid metabolism and growth in tissue‐isolated human leiomyosarcoma xenografts (2009)

Melatonin provides a circadian signal that regulates linoleic acid (LA)-dependent tumor growth. In rodent and human cancer xenografts of epithelial origin in vivo, melatonin suppresses the growth-stimulatory effects of linoleic acid (LA) by blocking its uptake and metabolism to the mitogenic agent, 13-hydroxyoctadecadienoic acid (13-HODE). This study tested the hypothesis that both acute and long-term inhibitory effects of melatonin are exerted on LA transport and metabolism, and growth activity in tissue-isolated human leiomyosarcoma (LMS), a rare, mesenchymally-derived smooth muscle tissue sarcoma, via melatonin receptor-mediated inhibition of signal transduction activity. Melatonin added to the drinking water of female nude rats bearing tissue-isolated LMS xenografts and fed a 5% corn oil (CO) diet caused the rapid regression of these tumors (0.17 +/- 0.02 g/day) versus control xenografts that continued to grow at 0.22 +/- 0.03 g/day over a 10-day period. LMS perfused in situ for 150 min with arterial donor blood augmented with physiological nocturnal levels of melatonin showed a dose-dependent suppression of tumor cAMP production, LA uptake, 13-HODE release, extracellular signal-regulated kinase (ERK 1/2), mitogen activated protein kinase (MEK), Akt activation, and [(3)H]thymidine incorporation into DNA and DNA content. The inhibitory effects of melatonin were reversible and preventable with either melatonin receptor antagonist S20928, pertussis toxin, forskolin, or 8-Br-cAMP. These results demonstrate that, as observed in epithelially-derived cancers, a nocturnal physiological melatonin concentration acutely suppress the proliferative activity of mesenchymal human LMS xenografts while long-term treatment of established tumors with a pharmacological dose of melatonin induced tumor regression via a melatonin receptor-mediated signal transduction mechanism involving the inhibition of tumor LA uptake and metabolism.
Multilingual extraction and mapping of dictionary entry names in business schema integration (2010)

Being a research field for many years, natural language processing (NLP) has gained a lot of attention in recent times due to the quality of translation software like Google Translator or Babylon. In the context of the iGreen project, we are developing a schema integration service (working title: Warp 10), which helps to generate business transformations from individual business schemata. To match the different schemas natural language processing plays an important role. Information coming from user input or files is extracted and mapped to a canonical data model (CDM) based on the CCTS standard. In this paper, we illustrate the use of NLP for the extraction of dictionary entry names (DENs) and indicate some of the problems of NLP and term extraction. Furthermore, we describe the NLP-supported mapping of DENs and outline the problems and approaches in a multilingual setting.
Mapping the genome (1993)

Article Free Access Share on Mapping the genome: some combinatorial problems arising in molecular biology Author: Richard M. Karp View Profile Authors Info & Claims STOC '93: Proceedings of the twenty-fifth annual ACM symposium on Theory of ComputingJune 1993 Pages 278–285https://doi.org/10.1145/167088.167170Published:01 June 1993Publication History 65citation1,101DownloadsMetricsTotal Citations65Total Downloads1,101Last 12 Months34Last 6 weeks9 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Familial influence on plaque formation in the beagle brain (1992)

Aged canines exhibit central neuropathological changes strikingly similar to those seen in patients with Alzheimer's disease. In this study, brain tissue from pure bred beagles raised in a controlled environment were examined for Alzheimer-like pathology. The mean age of the animals was 15.6 years. The incidence of plaques among these 29 dogs was 65.5%. Of the 19 samples that demonstrated Alzheimer-like pathology, 18 were characterized as diffuse and one as neuritic. Plaque density was found to be independent of age. Plaque numbers were highest in the perirhinal cortex and the adjacent temporal cortex. Familial influence on plaque development is supported by congruence within 15 of the 16 litters examined (p < 0.001). In this environmentally controlled group the diffuse plaques were rarely converted to the dense neuritic plaques found in Alzheimer's disease.
Incompatibility of Ifosfamide with Benzyl-Alcohol-Preserved Bacteriostatic Water for Injection (1988)

Journal Article Incompatibility of Ifosfamide with Benzyl-Alcohol-Preserved Bacteriostatic Water for Injection Get access Robert J. Behme, Robert J. Behme Research Scientist Pharmaceutical Research and Development Division, Bristol-Myers Company, Evansville, IN. Address reprint requests to Mr. Behme at Bristol-Myers Company, 2404 Pennsylvania Avenue, Evansville, IN 47721-0001. Search for other works by this author on: Oxford Academic Google Scholar Dana Brooke, Ph.D., Dana Brooke, Ph.D. Associate Director of Pharmaceutical Product Development and Adjunct Professor Pharmaceutical Research and Development Division, Bristol-Myers Company, School of Pharmacy, Purdue University, West Lafayette, IN. Search for other works by this author on: Oxford Academic Google Scholar Terry T. Kensler, Ph.D., Terry T. Kensler, Ph.D. Associate Director of Pharmaceutical Product Development Bristol-Myers Company Search for other works by this author on: Oxford Academic Google Scholar Joseph A. Scott, Ph.D. Joseph A. Scott, Ph.D. Manager Non-Oral Products Group, Pharmaceutical Research and Development Division, Bristol-Myers Company Search for other works by this author on: Oxford Academic Google Scholar American Journal of Hospital Pharmacy, Volume 45, Issue 3, 1 March 1988, Pages 627–628, https://doi.org/10.1093/ajhp/45.3.627 Published: 01 March 1988
Multi-view Transfer Learning with Adaboost (2011)

Transfer learning, serving as one of the most important research directions in machine learning, has been studied in various fields in recent years. In this paper, we integrate the theory of multi-view learning into transfer learning and propose a new algorithm named Multi-View Transfer Learning with Adaboost (MV-TL Adaboost). Different from many previous works on transfer learning, we not only focus on using the labeled data from one task to help to learn another task, but also consider how to transfer them in different views synchronously. We regard both the source and target task as a collection of several constituent views and each of these two tasks can be learned from every views at the same time. Moreover, this kind of multi-view transfer learning is implemented with adaboost algorithm. Furthermore, we analyze the effectiveness and feasibility of MV-TL Adaboost. Experimental results also validate the effectiveness of our proposed approach.
A thermal porosimetry method to estimate pore size distribution in highly porous insulating materials (2012)

Standard pore size determination methods such as mercury porosimetry, nitrogen sorption, microscopy, or x-ray tomography are not always applicable to highly porous, low density, and thus very fragile materials. For this kind of materials, a method based on thermal characterization is proposed. Indeed, the thermal conductivity of a highly porous and insulating medium is significantly dependent on the thermal conductivity of the interstitial gas that depends on both gas pressure and size of the considered pore (Knudsen effect). It is also possible to link the pore size with the thermal conductivity of the medium. Thermal conductivity measurements are realized on specimens placed in an enclosure where the air pressure is successively set to different values varying from 10(-1) to 10(5) Pa. Knowing the global porosity ratio, an effective thermal conductivity model for a two-phase air-solid material based on a combined serial-parallel model is established. Pore size distribution can be identified by minimizing the sum of the quadratic differences between measured values and modeled ones. The results of the estimation process are the volume fractions of the chosen ranges of pore size. In order to validate the method, measurements done on insulating materials are presented. The results are discussed and show that pore size distribution estimated by the proposed method is coherent.
ASIC-based development of cellular neural networks (2002)

The design and realization of cellular neural networks is discussed. An extension to the development environment NNSIM (neural network simulator) supports the creation of cellular networks. Furthermore the IC/sup 3/ ASIC cell library is introduced, allowing fast turn-around mixed hardware/software prototyping to detail real-time effects. A typical ASIC contains 150 neurons and 750 synapses.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Link Analysis in Web Information Retrieval. (2000)

The analysis of the hyperlink structure of the web has led to significant improvements in web information retrieval. This survey describes two successful link analysis algorithms and the state-of-the art of the field.
Diffractive-MEMS implementation of a hadamard near-infrared spectrometer (2005)

This paper describes the implementation of an optically efficient near-infrared spectrometer using a diffractive-MEMS chip in combination with digital-transform methods. This spectrometer architecture offers potential advantages over conventional detector-array-based designs because the modulation inherent in the use of digital-transform spectroscopy not only permits the normal signal-to-noise improvements associated with modulation of the light source, but it also creates an inherent insensitivity to stray light. Sample applications, illustrated here for the specific case of the Hadamard digital transform, include material inspection and identification and the monitoring of dynamic processes, such as epoxy-resin cure.
Route Development and Multikilogram GMP Delivery of a Somatostatin Receptor Antagonist (2012)

Route development and demonstration on multikilogram scale for the first GMP delivery of MK-4256 are described. Key aspects of the convergent route include a regioselective green iodination, one-pot oxadiazole synthesis, and an efficient ketone Pictet–Spengler reaction with diastereomeric upgrade via crystallization to afford 6 kg of API. A recycle procedure augmented the yield of desired diastereomer in the Pictet–Spengler reaction from a mixture of diastereomers heavily enriched in the undesired diastereomer.
Protein kinase A RII‐like (R2D2) proteins exhibit differential localization and AKAP interaction (2008)

A-kinase anchoring proteins (AKAPs) bind to protein kinase A (PKA) via an amphipathic helix domain that interacts with a dimerization/docking domain on the regulatory (R) subunit of PKA. Four other mammalian proteins (ROPN1, ASP, SP17, and CABYR) also contain a highly conserved RII dimerization/docking (R2D2) domain, suggesting all four proteins may interact with all AKAPs in a manner similar to RII. All four of these proteins were originally detected in the flagellum of mammalian sperm. In this report, we demonstrate that all four R2D2 proteins are expressed in a wide variety of tissues and three of the proteins SP17, CABYR, and ASP are located in motile cilia of human bronchus and fallopian tubes. In addition, we detect SP17 in primary cilia. We also provide evidence that ROPN1 and ASP bind to a variety of AKAPs and this interaction can be disrupted with anchoring inhibitor peptides. The interaction of SP17 and CABYR with AKAPs appears to be much more limited. None of the R2D2 proteins appears to bind cAMP, a fundamental characteristic of the regulatory subunits of PKA. These observations suggest that R2D2 proteins utilize docking interactions with AKAPs to accomplish their function of regulating cilia and flagella. Based on location, affinity for AKAPs and lack of affinity for cAMP, it appears that each R2D2 protein has a unique role in this process.
Indoleamine 2,3-dioxygenase activity and clinical outcome following induction chemotherapy and concurrent chemoradiation in Stage III non-small cell lung cancer (2013)

Indoleamine 2,3-dioxygenase (IDO) has recently been proposed to account for tumor-induced immunosuppression by influencing the conversion of tryptophan (Trp) into kynurenine (Kyn). The objective of our study was to correlate IDO activity with disease outcome in non-small cell lung cancer (NSCLC) patients treated with multimodal combination therapy. In a single-arm Phase II trial involving induction gemcitabine and carboplatin followed by concurrent paclitaxel, carboplatin and 74 Gy thoracic radiation in stage III NSCLC patients, plasma was drawn at baseline, post-induction, and post-concurrent therapy. The mean plasma Kyn/Trp ratio was used as a surrogate indicator of IDO activity. The 33 participants were distributed as follows: 15 females, 18 males; median age = 62; median overall survival (OS) = 22.4 (95% CI 19.3-25.1) months; median progression-free survival (PFS) = 11.5 (95% CI 6.7-16.3) months. The mean Kyn/Trp ratio at baseline (4.5 ± 2.8) was higher than that of healthy controls (2.9 ± 1.9, p = 0.03) and increased after induction therapy (5.2 ± 3.2, p = 0.08) and chemoradiation (5.8 ± 3.9, p = 0.01). The post-treatment Kyn/Trp ratio and radiologic responses were not significantly associated at any time point. No significant correlation was found between baseline Kyn/Trp ratios and OS (HR = 1.1, 95% CI 0.45-2.5) or PFS (HR = 0.74, 95% CI 0.30-1.82). A post-induction chemotherapy increase in IDO activity portended worse OS (HR = 0.43, 95% CI 0.19-0.95, p = 0.037) and PFS (HR = 0.47, 95% CI 0.22-1.0, p = 0.055). This observed increase in IDO transcription may be a means for tumors to evade immunosurveillance.