gebhart/patrepeval huggingface dataset:
Differential Immune Modulatory Activity of <i>Pseudomonas aeruginosa</i> Quorum-Sensing Signal Molecules (2004)

Pseudomonas aeruginosa releases a spectrum of well-regulated virulence factors, controlled by intercellular communication (quorum sensing) and mediated through the production of small diffusible quorum-sensing signal molecules (QSSM). We hypothesize that QSSM may in fact serve a dual purpose, also allowing bacterial colonization via their intrinsic immune-modulatory capacity. One class of signal molecule, the N-acylhomoserine lactones, has pleiotropic effects on eukaryotic cells, particularly those involved in host immunity. In the present study, we have determined the comparative effects of two chemically distinct and endobronchially detectable QSSM, N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) and 2-heptyl-3-hydroxy-4 (1H)-quinolone or the Pseudomonas quinolone signal (PQS), on human leukocytes exposed to a series of stimuli designed to detect differential immunological activity in vitro. 3-Oxo-C12-HSL and PQS displayed differential effects on the release of interleukin-2 (IL-2) when human T cells were activated via the T-cell receptor and CD28 (a costimulatory molecule). 3-Oxo-C12-HSL inhibited cell proliferation and IL-2 release; PQS inhibited cell proliferation without affecting IL-2 release. Both molecules inhibited cell proliferation and the release of IL-2 following mitogen stimulation. Furthermore, in the presence of Escherichia coli lipopolysaccharide, 3-oxo-C12-HSL inhibited tumor necrosis factor alpha release from human monocytes, as reported previously (K. Tateda et al., Infect. Immun. 64:37-43, 1996), whereas PQS did not inhibit in this assay. These data highlight the presence of two differentially active immune modulatory QSSM from P. aeruginosa, which are detectable endobronchially and may be active at the host/pathogen interface during infection with P. aeruginosa, should the bronchial airway lymphoid tissues prove to be accessible to QSSM.
Oscillator strength dependence of cavity-polariton mode splitting in semiconductor microcavities (1996)

We demonstrate the continuous alteration of the exciton-polariton mode splitting in semiconductor microcavities, through the change of the exciton oscillator strength induced by quantum confined Stark effect (QCSE). This is clear evidence of the direct influence of the oscillator strength on the mode splitting. A wide range variation, from 0 to 2 nm, of the splitting is obtained by the tuning of the cavity resonance based on the in-plane dispersion of the photon mode as well as the tuning of exciton energy by QCSE. The influence of the field-induced exciton line broadening on the mode splitting is also observed for the applied electric field as high as 90 kV/cm.
Laser Capture Microdissection of Cells from Plant Tissues (2003)

Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.
Direct recruitment of CRK and GRB2 to VEGFR-3 induces proliferation, migration, and survival of endothelial cells through the activation of ERK, AKT, and JNK pathways (2005)

Vascular endothelial growth factor receptor-3 (VEGFR-3) plays a key role for the remodeling of the primary capillary plexus in the embryo and contributes to angiogenesis and lymphangiogenesis in the adult. However, VEGFR-3 signal transduction pathways remain to be elucidated. Here we investigated VEGFR-3 signaling in primary human umbilical vein endothelial cells (HUVECs) by the systematic mutation of the tyrosine residues potentially involved in VEGFR-3 signaling and identified the tyrosines critical for its function. Y1068 was shown to be essential for the kinase activity of the receptor. Y1063 signals the receptor-mediated survival by recruiting CRKI/II to the activated receptor, inducing a signaling cascade that, via mitogen-activated protein kinase kinase-4 (MKK4), activates c-Jun N-terminal kinase-1/2 (JNK1/2). Inhibition of JNK1/2 function either by specific peptide inhibitor JNKI1 or by RNA interference (RNAi) demonstrated that activation of JNK1/2 is required for a VEGFR-3-dependent prosurvival signaling. Y1230/Y1231 contributes, together with Y1337, to proliferation, migration, and survival of endothelial cells. Phospho-Y1230/Y1231 directly recruits growth factor receptor-bonus protein (GRB2) to the receptor, inducing the activation of both AKT and extracellular signal-related kinase 1/2 (ERK1/2) signaling. Finally, we observed that Y1063 and Y1230/Y1231 signaling converge to induce c-JUN expression, and RNAi experiments demonstrated that c-JUN is required for growth factor-induced prosurvival signaling in primary endothelial cells.
Extraction and Assembly of Tissue-Derived Gels for Cell Culture and Tissue Engineering (2008)

Interactions with the extracellular matrix (ECM) play an important role in regulating cell function. Cells cultured in, or on, three-dimensional ECM recapitulate similar features to those found in vivo that are not present in traditional two-dimensional culture. In addition, both natural and synthetic materials containing ECM components have shown promise in a number of tissue engineering applications. Current materials available for cell culture and tissue engineering do not adequately reflect the diversity of ECM composition between tissues. In this paper, a method is presented for extracting solutions of proteins and glycoproteins from soft tissues and inducing assembly of these proteins into gels. The extracts contain ECM proteins specific to the tissue source with low levels of intracellular molecules. Gels formed from the tissue-derived extracts have nanostructure similar to ECM in vivo and can be used to culture cells as both a thin substrate coating and a thick gel. This technique could be used to assemble hydrogels with varying composition depending upon the tissue source, hydrogels for three-dimensional culture, as scaffolds for tissue engineering therapies, and to study cell-matrix interactions.
E4orf4, a Novel Adenovirus Death Factor That Induces p53-independent Apoptosis by a Pathway That Is Not Inhibited by zVAD-fmk (1998)

In the absence of E1B, the 289-amino acid product of human adenovirus type 5 13S E1A induces p53-independent apoptosis by a mechanism that requires viral E4 gene products (Marcellus, R.C., J.C. Teodoro, T. Wu, D.E. Brough, G. Ketner, G.C. Shore, and P.E. Branton. 1996. J. Virol. 70:6207-6215) and involves a mechanism that includes activation of caspases (Boulakia, C.A., G. Chen, F.W. Ng, J. G. Teodoro, P.E. Branton, D.W. Nicholson, G.G. Poirier, and G.C. Shore. 1996. Oncogene. 12:529-535). Here, we show that one of the E4 products, E4orf4, is highly toxic upon expression in rodent cells regardless of the p53 status, and that this cytotoxicity is significantly overcome by coexpression with either Bcl-2 or Bcl-XL. Conditional expression of E4orf4 induces a cell death process that is characterized by apoptotic hallmark features, such as externalization of phosphatidylserine, loss of mitochondrial membrane potential, cytoplasmic vacuolation, condensation of chromatin, and internucleosomal DNA degradation. However, the wide-spectrum inhibitor of caspases, tetrapeptide zVAD-fmk, does not affect any of these apoptogenic manifestations, and does not alter the kinetics of E4orf4-induced cell death. Moreover, E4orf4 expression does not result in activation of the downstream effector caspase common to most apoptosis-inducing events, caspase-3 (CPP32). We conclude, therefore, that in the absence of E1A, E4orf4 is sufficient by itself to trigger a p53-independent apoptosis pathway that may operate independently of the known zVAD-inhibitable caspases, and that may involve an as yet uncharacterized mechanism.
Redox chemiluminescence detector: application to gas chromatography (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRedox chemiluminescence detector: application to gas chromatographyStefan A. Nyarady, Robert M. Barkley, and Robert E. SieversCite this: Anal. Chem. 1985, 57, 11, 2074–2079Publication Date (Print):September 1, 1985Publication History Published online1 May 2002Published inissue 1 September 1985https://pubs.acs.org/doi/10.1021/ac00288a016https://doi.org/10.1021/ac00288a016research-articleACS PublicationsRequest reuse permissionsArticle Views146Altmetric-Citations30LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Autonomous navigation based on a Q-learning algorithm for a robot in a real environment (2008)

This paper explores autonomous navigation and obstacle avoidance techniques based on Q-learning for a mobile robot in a real environment. The implemented algorithm focuses on simplicity and efficiency. The learning process takes place in both simulation and real world allowing the combination of a longer learning time in the simulator with a more accurate knowledge from the real world. After learning is completed in simulation and in the real world, the robot was able to navigate without hitting obstacles and able to generate control law for complex situations such as corners and small objects.
Picosecond temporal resolution photoemissive sampling (1987)

Sampling measurements of electrical transients generated photoconductively on a 5-μm gold coplanar transmission line on GaAs were performed via multiphoton photoemission. A temporal resolution of 5 ps was achieved, with a voltage sensitivity of 10 mV/(Hz)1/2. These results confirm that the temporal resolution attainable by this technique is enhanced when the dimensions of the structure under examination are reduced.
Scalable compositional minimization via static analysis (2005)

State-equivalence based reduction techniques, e.g. bisimulation minimization, can be used to reduce a state transition system to facilitate subsequent verification tasks. However, the complexity of computing the set of equivalent state pairs often exceeds that of performing symbolic property checking on the original system. We introduce a fully-automated efficient compositional minimization approach which requires only static analysis. Key to our approach is a heuristic algorithm that identifies components with high reduction potential in a bit-level netlist. We next inject combinational logic which restricts the component's inputs to selected representatives of symbolically-computed equivalence classes thereof. Finally, we use existing transformations to synergistically exploit the dramatic netlist reductions enabled by these input filters. Experiments confirm that our technique is able to efficiently yield substantial reductions on large industrial netlists.
In Situ Detection of Live Cancer Cells by Using Bioprobes Based on Au Nanoparticles (2008)

We fabricate the high-performance probes based on Au nanoparticles (AuNP) for detection of live cancer cell. AuNP were synthesized with narrow sized distribution (ca. 10 nm) by Au salt reduction method and deposited onto the aminated substrate as a cross-linker and hot spot. Herein, AuNP has enabled the easy and efficient immobilization of the antibody (Cetuximab), which can selectively interact with epidermal growth factor receptor (EGFR) on the surface of epidermal cancer, as detecting moiety onto the AuNP-deposited substrate without nanolithography process. After conjugation of Cetuximab with AuNP-deposited substrate, Cetuximab-conjugated probe as a live cancer cell detector (LCCD) could detect EGFR-highexpressed A431 cells related to epithelial cancer with 54-times larger specificity and sensitivity in comparison with EGFR-deficient MCF7 cells. This implies that AuNP-based probes demonstrate abundant potentials for detection and separation of small biomolecules, cells and other chemicals.
A variational formulation for frame-based inverse problems (2007)

A convex variational framework is proposed for solving inverse problems in Hilbert spaces with a priori information on the representation of the target solution in a frame. The objective function to be minimized consists of a separable term penalizing each frame coefficient individually and of a smooth term modeling the data formation model as well as other constraints. Sparsity-constrained and Bayesian formulations are examined as special cases. A splitting algorithm is presented to solve this problem and its convergence is established in infinite-dimensional spaces under mild conditions on the penalization functions, which need not be differentiable. Numerical simulations demonstrate applications to frame-based image restoration. 1
Giant spontaneous Hall effect in zero-moment Mn2Ru<i>x</i>Ga (2015)

Spin-dependent transport properties of Mn2RuxGa thin-films are studied as function of the Ru concentration and the substrate-induced strain. The large spontaneous Hall angle of 7.7% twenty times bigger than in other 3d metals is a signature of its half-metallicity. The compensation temperature where the magnetization of the two inequivalent antiferromagnetically coupled Mn sublattices cancel can be tuned by varying x or the biaxial strain. This zero-moment half metal is free from demagnetizing forces and creates no stray field, effectively removing two obstacles to integrating magnetic elements in densely packed, nanometer-scale memory elements, and millimeter-wave generators.
A monoclonal antibody-enzyme immunoassay for serum carcinoembryonic antigen with increased specificity for carcinomas. (1983)

A two-site monoclonal antibody-enzyme immunoassay (MEIA) for carcinoembryonic antigen (CEA) was developed that uses two monoclonal anti-CEA antibodies, which recognize two different epitopes in the peptide moiety of CEA. The assay was sensitive to 0.5 micrograms/liter and had a measuring range of 0.5-200 micrograms of CEA per liter. It was highly specific inasmuch as none of three known CEA-related substances, "nonspecific crossreacting antigens 1 and 2" (NCA-1 and NCA-2) and biliary glycoprotein I (BGP I), reacted in the assay. NCA-2 (meconium antigen) is very similar to CEA. None of five commercially available CEA assays were able to differentiate between CEA and NCA-2. With one exception (colon), normal tissue extracts did not react in the MEIA even when tested at very high concentrations. Sera from a total of 180 healthy individuals and patients with malignant and nonmalignant disease were analyzed for CEA levels by using the MEIA and in parallel a conventional radioimmunoassay. A significant increase in specificity for carcinomas was obtained with the MEIA. This was essentially due to a decrease of MEIA CEA values in sera from patients with nonmalignant disease. The CEA values in the group of carcinoma patients (colon, pancreas, lung, and breast) were the same in the two assays.
Workshop on Computational Personality Recognition: Shared Task (2021)

In the Workshop on Computational Personality Recognition (Shared Task), we released two datasets, varying in size and genre, annotated with gold standard personality labels. This allowed participants to evaluate features and learning techniques, and even to compare the performances of their systems for personality recognition on a common benchmark. We had 8 participants to the task. In this paper we discuss the results and compare them to previous literature.
Development of compact broadband receivers at submillimeter wavelengths (2004)

The development of compact broadband fixed-tuned receivers at millimeter and submillimeter wavelengths is discussed. These components rely on a fusion between novel integrated planar diode technology and innovative broadband circuit topologies. A 500 GHz InGaAs subharmonic mixer has been demonstrated that has the same sensitivity as a GaAs mixer (T/sub MIX//spl sim/1500 K (DSB)) while using less than half the LO power. In addition, an integrated tripler-mixer has been developed at 180 GHz that exhibits state-of-the-art sensitivity (T/sub MIX//spl sim/550 K (DSB)) using 125 mW of LO power at 30 GHz. The ultimate goal of this research is to develop a compact, highly sensitive radiometer at 874 GHz to meet upcoming critical NASA Earth Science requirements.
Improved performance of sulfonated polyarylene ethers for proton exchange membrane fuel cells (2006)

Abstract The performances of polyarylene ethers and sulfonated polyarylene ethers having different backbone structures and sulfonated position were comparatively investigated focusing on the determination of the proton conductivity, thermal and chemical stability. The results indicated that the sulfone groups in polymer backbones are highly thermal stable. The sulfonic acid group at the meta ‐sulfone position had better thermal, chemical stability and higher proton conductivity than those at the ortho ‐ether and ortho ‐sulfone position. The series of stability is meta ‐sulfone sulfonated polyphenyl sulfone (SPSU) > ortho ‐sulfone SPSU ≫ ortho ‐ether SPSU. The knowledge of these parameters will lead to the synthesis of sulfonated arylene main‐chain ionomers that are optimized for application in fuel cells. Also different sulfonated polyphenyl sulfone‐4,4′‐biphenol/polybenzimidazole (SPSU‐BP/PBI) acid‐base crosslinked blend membranes have been studied. The SPSU/PBI crosslinked blend membranes had better mechanical stability than their parent membrane while maintaining good thermal stability and having suitable proton conductivity. Copyright © 2006 John Wiley & Sons, Ltd.
Reaching the next level of indoor human presence detection: An RF based solution (2013)

The presence of a human in the vicinity of radio transceivers results in radio signal strength variations at the receiver's input. Therefore, human presence in an indoor environment can be recognized by analyzing and quantifying irregularities in the radio signature. In order to quantify the information in terms of human presence, we present a novel human presence detection method based on information entropy extracted from a sequence of received signal strength samples. As opposed to existing smart home solutions that incorporate a complex set of sensors for human detection, the proposed method is solely based on radio irregularity phenomenon, without modifying the original environment. An additional level of sensor intelligence is introduced without sensors and installation costs, or specific training procedures for end-consumers.
Machine quantification of text-based economic reports for use in predictive modeling (2004)

To quantify text-based unstructured information, we propose a method called the direct scoring algorithm (DSA). DSA uses keywords in the document, subjectively-determined numerical weights, and subjectively-designed grammar rules to score individual sentences. We use our methods to score the Beige books produced by the U.S. Federal Reserve, which contain subjective text-based commentary on state of the economy. To assess whether our scores have value in a predictive sense, we use them to construct a linear regression model of future growth in U.S. gross domestic product (GDP). We then compare the performance characteristics of this model with those a similar model based on scores of the same documents produced though subjective reading by professional economists. The comparison demonstrates that the DSA model using the Beige book significantly contributes to the prediction of GDP growth, explaining as much as 69% of the variance compared to the scores created by economic experts. We also add the extracted section scores to a GDP time series prediction model, which uses only structured data as input. The results of this experiment suggest the unstructured information in the Beige books has predictive value that goes beyond that of the structure information used in the time series model, and that our approach has some potential as a means of extracting this information in a semi-automated fashion.
3K-4 Acoustic Wave Generation and Detection in Non-Piezoelectric High-Q Resonators (2006)

Acoustic sensing is traditionally based on the piezoelectric effect and thus limited in the selection of transducer materials and options for sensor fabrication. Another excitation method is the principle of magnetic direct generation of acoustic waves, which has been employed for non-destructive evaluation (NDE) of materials as electromagnetic-acoustic transducer (EMAT) sensors for several decades. With a suitable high-g mechanical resonator the lower transduction efficiency can be overcome. The advantages of remote excitation, i.e., inexpensive fabrication of many different resonator materials and a wide variety of possible vibration modes, can be utilized to suit the specific needs of an application. Latest developments concentrate on the characterization of different excitation setups to generate and compare different modes of vibration
Web Infrastructure for the 21st Century (2009)

The Web success in leading the information technology revolution has relied on an enormous computing infrastructure. In recent years, both cloud computing as well as social networks are putting even more burden in such infrastructure. The cloud computing paradigm is creating a massive shift in computing – from PC-based applications to cloud-based applications. Cloud computing frees users from having to remember where the data resides, gives users access to information anywhere, and provides fast services through essentially infinite online computing. Social networks, on the other hand, emerge the social aspects of the Web where the social interactions put demands on Web applications, and in turn further demands in the Web's infrastructure. 

The Internet, which was mostly developed for interactive applications between humans and computers, has struggled to handle the necessities of a Web designed around content. For instance, as Web content moves from one place to another, Web pointers are broken and so do search ranking algorithms. Similarly, content is often not where it should be when you need it and routers waste capacity copying the same content millions of times. As a result, we have seen the emergence of Internet systems that were not planned for from the beginning, e.g. Web Caching, Content Distribution Networks, or P2P networks.

In this talk I will discuss the challenges that the Web is posing in today's Internet infrastructure, and argue about various solutions to cope with them. In particular, I will argue how to re-think the Internet to do networking at the content/information layer, and the underlying architectural system design principles needed to guide the efficient engineering of new Web infrastructure services.