gebhart/patrepeval huggingface dataset:
Coordinated control of two robot arms (1986)

A new control method is presented for the coordinated control of two robot arms based on exact system linearization by appropriate non-linear feedback. The two arms are assumed to be working on the same object simultaneously. The control method uses a dynamic coordinator acting on relative position and velocity error and/or on relative force-torque errors between the two arms. Simulation results are presented for the coordinated control of two PUMA 560 robot arms using an optimal dynamic coordinator. For completeness, the paper also presents (i) a new revised set of inertia parameters for all six links of PUMA 560 robot arm, (ii) the form of nonlinear feedback and diffeomorphic state transformation which linearizes the control of PUMA 560 working in all six degrees of freedom, and (iii) the reduced numeric form of all dynamic equations for PUMA 560 working in three (positioning) degrees of freedom.
Expression of Fc <i>mu</i> receptors by human T lymphocytes: effects of enzymes, metabolic inhibitors, and X-irradiation. (1981)

Abstract The expression of Fcµ receptors by purified human peripheral blood T lymphocytes during culture was studied using a rosette technique with rabbit IgM antibody-coated ox erythrocytes (EOx-IgM). Unbound Fcµ receptors were not detected on freshly isolated purified T cells before or after treatment with 50 U/ml neuraminidase (Vibrio comma). Optimal detection of unbound receptors required culture of viable T cells for 24 hr at 37°C in RPMI-1640 medium with FCS, or in Iscove’s medium without FCS. Freshly isolated T cells treated with sodium azide did not express Fcµ receptors, nor did T cells cultured at 4°C. A reduced number of T cells expressed Fcµ receptors after incubation in RPMI-1640 medium without FCS. A direct influence of T cell protein synthesis on Fcµ receptor expression is likely, since T cells cultured with puromycin or cycloheximide or briefly treated with actinomycin D or mitomycin C prior to culture displayed parallel temporal inhibition of EOx-IgM rosette formation and 3H-leucine incorporation. Freshly isolated T cells irradiated with 500 to 5000 R before culture also displayed parallel temporal inhibition of EOx-IgM rosette formation and 3H-leucine incorporation. No impairment of optimal EOx-IgM rosette formation occurred in control T lymphocytes cultured for 24 hr then treated with metabolic inhibitors or x-irradiation before rosetting, which suggests that their effects did not directly influence the receptor-ligand interaction itself. However, treatment of these cultured control T cells with trypsin or IgM but not neuraminidase before rosetting subsequently decreased these cells’ EOx-IgM rosette formation. These findings suggest the expression of Fcµ receptors by cultured T cells as detected by EOx-IgM rosette formation is critically dependent on intracellular events, principally protein synthesis, whereas the interaction of receptor and ligand leading to rosette formation is principally dependent on the absence of a competitive ligand, i.e., unbound IgM.
Assist-as-needed training paradigms for robotic rehabilitation of spinal cord injuries (2006)

This paper introduces a new "assist-as-needed" (AAN) training paradigm for rehabilitation of spinal cord injuries via robotic training devices. In the pilot study reported in this paper, nine female adult Swiss-Webster mice were divided into three groups, each experiencing a different robotic training control strategy: a fixed training trajectory (fixed group, A), an AAN training method without interlimb coordination (Band Group, B), and an AAN training method with bilateral hind-limb coordination (Window Group, C). Fourteen days after complete transection at the mid-thoracic level, the mice were robotically trained to step in the presence of an acutely administered serotonin agonist, quipazine, for a period of six weeks. The mice that received AAN training (Groups B and C) show higher levels of recovery than Group A mice, as measured by the number, consistency, and periodicity of steps realized during testing sessions. Group C displays a higher incidence of alternating stepping than Group B. These results indicate that this training approach may be more effective than fixed trajectory paradigms in promoting robust post-injury stepping behavior. Furthermore, the constraint of interlimb coordination appears to be an important contribution to successful training
Surface-roughness-induced contradirectional coupling in ring and disk resonators (1997)

Reflections that are due to random surface roughness in periodic structures such as dielectric rings and disks inherently phase match forward- and backward-propagating modes. Small reflections are thus considerably enhanced and may impair the performance of traveling-wave resonators. In addition, such contradirectional coupling leads to a splitting of the resonant peak. These effects are studied analytically.
Conditional ligands for <scp>A</scp>sian <scp>HLA</scp> variants facilitate the definition of <scp>CD</scp>8<sup>+</sup><scp>T</scp>‐cell responses in acute and chronic viral diseases (2012)

Conditional ligands have enabled the high-throughput production of human leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring platforms typically concentrate on restriction elements associated with European ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel irradiation-sensitive ligands, specifically targeting South East Asian populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, respectively. Unique ligands for all 16 HLA types were constructed to provide the desired soluble HLA product in sufficient yield. Peptide exchange was accomplished for all variants as demonstrated by an ELISA-based MHC stability assay. HLA tetramers with redirected specificity could detect antigen-specific CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this population-centric HLA library was demonstrated with the characterization of seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, HBV, and DENV. Posthoc analysis revealed that the majority of responses would be more readily identified by our unbiased discovery approach than through the application of state-of-the-art epitope prediction. This flow cytometry-based technology therefore holds considerable promise for monitoring clinically relevant antigen-specific T-cell responses in populations of distinct ethnicity.
Dyspepsia world-wide and the role of stress (1997)

From the Division of Gastroenterology, Ottawa Civic Hospital, University of Ottawa, Ontario, Canada. Requests for reprints to W. Grant Thompson, Division of Gastroenterology, A1, Ottawa Civic Hospital, 1053 Carling Avenue, Ottawa, Ontario, Canada K1Y 4E9. Date received: 20 October 1996.
EFFECTS OF DIMETHYL SULFOXIDE ON SUBUNIT PROTEINS* (1975)

The effects of DMSO are thought to result from the formation of hydrogen bonds with proton-donor groups on biopolymers, which are stronger than those formed with water. Since DMSO contains methyl groups, however, effects on hydrophobic bonding in proteins could be expected at higher DMSO levels. Our studies of the effects of DMSO on model subunit proteins can be interpreted in the above terms. At a concentration of 20% or less, DMSO changed glutamate dehydrogenase into the inactive monomer and the effects were fully reversible with the activator (ADP). Higher DMSO levels resulted in irreversible inactivation. The predominant effect noted on beta-glucuronidase was irreversible inactivation by 20% or more DMSO at 37 degrees C. Purified beta-glucuronidase exhibited an activation in 20% DMSO at high substrate levels; this resulted from an apparent substrate inhibition in the absence of DMSO. DMSO inhibited the clotting of fibrinogen by purified thrombin, but the major effect appeared to be due to competition between thrombin and DMSO for binding sites on fibrinogen. These effects appear to be largely due to interactions between DMSO and hydrophobic bonding in fibrinogen, although DMSO also appears to interfere with the aggregation of fibrin monomers through its effects on hydrophilic groups. These results suggest that reversible alterations in protein structure are the major effect of exposure of subunit proteins to low DMSO levels at low temperatues, while irreversible denaturation of subunit proteins may be an appreciable effect a higher temperatures and higher DMSO concentrations.
Infant intestinal Enterococcus faecalis down-regulates infl ammatory responses in human intestinal cell lines (2008)

These data provide a case for the modulation of intestinal mucosal immunity in which specific strains of E. faecalis have uniquely evolved to maintain colonic homeostasis and regulate inflammatory responses.
State estimation via multiple observer with unknown inputs : Application to the three tank system (2003)

In this paper, we consider a nonlinear system represented by a multiple model, where a part of its inputs is unknown. Our objective is to estimate the state variables of this system. For that, we propose the synthesis of a multiple observer based on the elimination of these unknown inputs. It is shown how to determine the gains of the local observers, these gains being solutions of a set of linear matrix inequalities (LMI). The model of an hydraulic system with three tanks is used to test the suggested procedure.
Ultramicrodetermination of Arsenic by Gutzeit Spot-Filtration under Vacuum. A Rapid Technique Employing Photometric Calibration and Permanent Photographic Standards (1944)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUltramicrodetermination of Arsenic by Gutzeit Spot-Filtration under Vacuum. A Rapid Technique Employing Photometric Calibration and Permanent Photographic StandardsHenry S. Satterlee and Gertrude. BlodgettCite this: Ind. Eng. Chem. Anal. Ed. 1944, 16, 6, 400–407Publication Date (Print):June 1, 1944Publication History Published online1 May 2002Published inissue 1 June 1944https://pubs.acs.org/doi/10.1021/i560130a027https://doi.org/10.1021/i560130a027research-articleACS PublicationsRequest reuse permissionsArticle Views61Altmetric-Citations11LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Amino acid sequences of α-helical segments from <i>S</i>-carboxymethylkerateine-A. Tryptic and chymotryptic peptides from a type-II segment (1978)

1. Amino acid-sequence studies were done on a peptide of mol.wt. approx. 12500 that was isolated from the highly helical fragments obtained by partial chymotryptic digestion of the low-sulphur proteins (S-carboxymethylkerateine-A) from wool. 2. The peptides obtained by tryptic and chymotryptic digestion of this large peptide were separated by ion-exchange chromatography on DEAE-cellulose at pH8.5 with an (NH4)(2)CO(3) concentration gradient and, where necessary, purified further by paper electrophoresis. 3. Determination of the sequences of many of these peptides showed that a high proportion of the cationic residues occurs in pairs. 4. Although two of the four S-carboxymethylcysteine residues are located in what appears to be a non-helical region near the N-terminus the other two S-carboxymethylcysteine residues occur in or near sequences suggesting a helical conformation. 5. Some peptides were obtained, in low yields, that appeared to be homologues of more major ones. These suggest either homologies in the helical portions of the low-sulphur proteins or the presence of closely related amino acid sequences in helical regions of completely different origins. 6. A partial sequence of the complete peptide is proposed.
Trypanosoma theileri: antibody-dependent killing by purified populations of bovine leucocytes. (1982)

Bovine leucocytes were assayed for their cytotoxic activity against Trypanosoma theileri, a large haemoflagellate parasite of cattle. Cytotoxicity was assessed by 3H-uridine release from pre-labelled parasites and also by light microscopy. Cytotoxicity proved to be totally dependent upon the presence of specific antibody. Serum and the immunoglobulin fraction of colostrum from normal adult cattle and serum from normal colostrum fed calves mediated cytotoxicity; serum from SPF colostrum deprived calves possessed no cytotoxic activity. Neutrophils, eosinophils, monocytes and macrophages obtained from both the peripheral blood and mammary gland of heifers were cytotoxic to T. theileri epimastigotes. Lymphocytes failed to mediate cytotoxicity either in the presence or absence of specific antibody. Despite the large size of this trypanosome all effector cells phagocytosed T. theileri. Phagocytosis by macrophages and eosinophils preceded specific isotope release, however neutrophils mediated 50% specific isotope release during the phagocytic period, suggesting extracellular cell-mediated lysis.
Scaffolding pre-assembled contigs using SSPACE (2010)

De novo assembly tools play a main role in reconstructing genomes from next-generation sequencing (NGS) data and usually yield a number of contigs. Using paired-read sequencing data it is possible to assess the order, distance and orientation of contigs and combine them into so-called scaffolds. Although the latter process is a crucial step in finishing genomes, scaffolding algorithms are often built-in functions in de novo assembly tools and cannot be independently controlled. We here present a new tool, called SSPACE, which is a stand-alone scaffolder of pre-assembled contigs using paired-read data. Main features are: a short runtime, multiple library input of paired-end and/or mate pair datasets and possible contig extension with unmapped sequence reads. SSPACE shows promising results on both prokaryote and eukaryote genomic testsets where the amount of initial contigs was reduced by at least 75%.
A new digital correlator for bandlimited direct sequence spread spectrum signals (2002)

A new digital correlator for the reception of bandlimited direct sequence (DS) spread spectrum (SS) signals is described. This correlator could be used in CDMA applications. The correlator is simple in its implementation, using a sign-controlled switch instead of the multiplier and a delta-sigma converter with up/down counter as an integrator. This A/D is simple in its realization, and does not require high-precision elements. Simulation is used to find the effects of the delta-sigma converter parameters on the system performance. Simulation results show that the new correlator, which is much simpler and has a lower cost than the matched filter receiver, has an error probability nearly equal to that of the matched filter for different types of interfering signals and multiuser interference for users using orthogonal signatures.
Chemically Cross‐Linked Poly(2‐hydroxyethyl methacrylate)‐Supported Deep Eutectic Solvent Gel Electrolytes for Eco‐Friendly Supercapacitors (2017)

Abstract Nonvolatile, ion‐dense electrolytes, such as ionic liquids and deep eutectic solvents (DESs) are attractive candidates for safe, high‐performance electrochemical/electrostatic energy storage devices. In this report, an inexpensive and eco‐friendly DES (1 : 2 mixture of choline chloride/ethylene glycol) has been incorporated into a solid‐state gel electrolyte using a chemically cross‐linked polymer scaffold formed through in situ UV copolymerization of 2‐hydroxyethyl methacrylate (HEMA) and poly(ethylene glycol) diacrylate. A high room‐temperature ionic conductivity of 5.7 mS cm −1 has been obtained for a DES gel containing 13.2 vol % polymer scaffold. Tunable DES gel compressive elastic modulus values, between 14 kPa and 1.0 MPa, could be obtained by varying the polymer content. Clear evidence of hydrogen‐bonding interactions between the poly(HEMA) scaffold and the DES anion was revealed through species self‐diffusivity measurements as well as by FTIR spectroscopy. Using a 22.9 vol % polymer‐supported DES gel as electrolyte/separator in a supercapacitor prototype with activated carbon fabric electrodes, a specific capacitance of 33.3 F g −1 and an energy density of 15.8 Wh kg −1 (based on the mass of two electrodes) were obtained during discharge at a current density of 0.01 A g −1 . This work supports the notion that chemically cross‐linked polymer‐supported DES gel electrolytes are suitable for solid‐state, flexible supercapacitor applications.
Acyclovir in hepatitis B antigen—positive chronic liver disease: inhibition of viral replication and transient renal impairment with iv bolus administration (1983)

Six patients with hepatitis B virus (HBV) related chronic liver disease were treated with acyclovir, 5-15 mg/kg 8 hourly, given as an iv bolus or iv infusion over 1 h for up to 7 days. Two patients treated with 10 and 15 mg/kg 8 hourly showed a decrease in HBV-DNA polymerase and HBV-DNA when mean trough acyclovir plasma concentrations of 5.0 +/- 0.6 and 13.2 +/- 3.0 microM were attained. Inhibition of viral replication was not seen in patients treated with lower doses. Transient renal impairment was seen in two patients who received high dosage by the iv bolus mode of administration. This complication may be prevented by a high oral fluid intake or iv infusion of the drug over 1 h. Further study with acyclovir 15 mg/kg 8-hourly given as an iv infusion for longer periods is warranted.
Low cost illumination computation using an approximation of light wavefronts (1994)

We present an efficient method to simulate the propagation of wavefronts and approximate the behavior of light in an environment of freeform surfaces. The proposed method can emulate the behavior of a wavefront emanating from a point or spherical light source, and possibly refracted and/or reflected from a freeform surface. Moreover, it allows one to consider and to render images with extreme illumination conditions such as caustics.
한중수교 20년과 한중군사관계 발전: 회고와 전망 (2012)

군사관계는 국가관계의 우호수준을 가늠할 수 있는 척도가 된다. 지난 20년을 회고해 볼 때 한중 군사관계는 정치, 외교, 경제 및 사회관계에 비해 그 발전 정도 가 훨씬 뒤떨어져 있었다. 한중 군사관계에는 여러 제약요인이 작용한다. 우선 한 반도를 둘러싼 냉전의 잔재가 아직 강하게 남아 있어 한중 양국 정부 간에 불협화 음을 일으키는 요인으로 작용한다. 2010년 있었던 천안함 피격 사건과 연평도 포 격 사건에 대한 한중 간의 이견과 외교적 마찰이 그 대표적인 사례이다. 한중 군사 협력을 `전략적 협력`의 수준으로까지 촉진시키기 위해서는 다음과 같은 방안을 고려할 수 있다. 첫째, 양국의 관계발전을 위한 접근방식으로 지금까지 사용되었던 `구동존이(求同存異)`는 `구동존리(求同尊利)`로 전환되어야 한다. 즉, 서로 다른 견해를 묻어두는 소극적 방식에서 벗어나, 상대방의 이익을 존중해주는 보다 적극 적인 태도를 견지해야 한다. 둘째로 한중 양국은 군 고위급 교류시 논의할 수 있는 의제를 보다 다양화해야 한다. 그래서 보다 전략적 가치를 갖는 현안에 대해 견해 를 교환할 필요가 있다. 셋째, 한중 양국은 지역내 다자협력을 확대해 나가야 한다. 가령 한국과 중국 정부는 양국 군간의 협력을 활성화 한 후 이를 확대하여 `동북아 국방대화체`를 만들기 위해 공동으로 힘을 합할 수 있다. 한중 군사관계 발전은 비단 양국관계 발전뿐만 아니라 동북아 지역차원에서의 평화와 안정에 밑거름을 제공할 것이다.
ChemInform Abstract: Anion‐Receptor Molecules: Synthesis of a Chiral and Functionalized Binding Subunit, a Bicyclic Guanidinium Group Derived from L‐ or D‐Asparagine. (1988)

Abstract The optically active (S,S)‐title compound (IX) is synthesized as its hydrochloride from the asparagine derivative (II) in a total yield of 5.1% from L‐asparagine (I).
Circulating Tumor-reactive CD8(+) T cells in melanoma patients contain a CD45RA(+)CCR7(-) effector subset exerting ex vivo tumor-specific cytolytic activity. (2002)

To defend the host from malignancies, the immune system can spontaneously raise CD8(+) T-cell responses against tumor antigens. Investigating the functional state of tumor-reactive cytolytic T cells in cancer patients is a key step for understanding the role of these cells in tumor immunosurveillance and for evaluating the potential of immunotherapeutic approaches of vaccination against cancer. In this study we identified a subset of circulating tumor-reactive CD8(+) T lymphocytes, which specifically secreted IFN-gamma after exposition to autologous tumor cell lines in stage IV metastatic melanoma patients. Additional phenotypic characterization using multicolor flow cytometry revealed that a significant fraction of these cells were CD45RA(+)CCR7(-), a phenotype that has been proposed recently to characterize cytolytic effectors potentially able to home into inflamed tissues. In the case of an HLA-A2-expressing patient, the antigen specificity of this population was identified by using HLA-A2/peptide multimers incorporating a tyrosinase-derived peptide. Consistently with their phenotypic characteristics, A2/tyrosinase peptide multimer(+) CD8(+) T cells, isolated by cell sorting, were directly lytic ex vivo and able to specifically recognize tyrosinase-expressing tumor cells. Overall, these results provide the first evidence that a proportion of melanoma patients have circulating tumor-reactive T cells, which are lytic effectors cells.
Multiplex coherent anti-Stokes Raman scattering (MCARS) for chemically sensitive, label-free flow cytometry (2009)

Flow cytometry is an ever-advancing high-throughput multivariate analysis tool that natively provides size and morphological information. To obtain molecular information, however, typically requires the addition of fluorophores, which are limited by spectral overlap, nonspecific binding, available conjugation chemistries, and cellular toxicity. A complementary or alternative, label-free approach to molecular information is through multiplex coherent anti-Stokes Raman scattering (MCARS), which is a coherent, nonlinear optical method that provides a wealth of molecular information by probing the Raman energies within a molecule. In this work, we demonstrate the unique capability of our MCARS flow cytometer to distinguish flowing particles and discuss system performance capabilities and possibilities.
Chemical Composition and Antimicrobial Variability of<i>Satureja montana</i>L. Essential Oils Produced During Ontogenesis (2004)

Abstract The essential oils obtained from Satureja montana L. harvested in central part of Dalmatia (Croatia) at three ontogenical stages were evaluated for their chemical composition and antimicrobial activity. GC/MS analyses revealed the presence of 33 compounds in the oils. Carvacrol (52.4>26.2>16.1%) was found to be the main constituent especially before flowering while p-cymene (3.8<15.2<25.6%) increased through flowering. The antimicrobial activity of S. montana oils against five Gram-negative bacteria (Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi), four Gram-positive bacteria (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Streptococcus faecalis) and five pathogenic fungi (Aspergillus fumigatus, Aspergillus niger, Candida albicans, Candida rugosa and Saccharomyces cerevisiae) was evaluated using the agar-plate dilution assay. The oil showed the strongest activity against all tests strains, with the exception of Pseudomonas aeruginosa. The maximum activity was observed against E. coli, S. aureus and fungal organisms. Escherichia coli had the lowest MIC of 0.06% with oils produced before and during the flowering period. These results support the notion that S. montana oil has a role as both a pharmaceutical and a preservative.
Whole Body Vibration Effects on Body Composition in the Postmenopausal Korean Obese Women: Pilot Study (2011)

WBV might display a weak but positive effect on body weight and waist circumference reduction in healthy postmenopausal obese women. However, attention must be given to avoid a decrease of muscle mass.