gebhart/patrepeval huggingface dataset:
Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 (2014)

The aromatic compound catechol is used as a precursor of chemical products having multiple applications. This compound is currently manufactured by chemical synthesis from petroleum-derived raw materials. The capacity to produce catechol is naturally present in several microbial species. This knowledge has been applied to the generation of recombinant Escherichia coli strains that can produce catechol from simple carbon sources. Several strains derived from E. coli W3110 trpD9923, a mutant that overproduces anthranilate, were modified by transforming them with an expression plasmid carrying genes encoding anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. The additional expression of genes encoding a feedback inhibition resistant version of 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase and transketolase from E. coli, was also evaluated. Generated strains were characterized in complex or minimal medium in shake-flask and fed-batch bioreactor cultures and incubation temperatures ranging from 37 to 28°C. These experiments enabled the identification of culture conditions for the production of 4.47 g/L of catechol with strain W3110 trpD9923, expressing 1,2-dioxygenase, DAHP synthase and transketolase. When considering the amount of glucose consumed, a yield of 16% was calculated, corresponding to 42% of the theoretical maximum as determined by elementary node flux analysis. This work demonstrates the feasibility of applying metabolic engineering for generating E. coli strains for the production of catechol from glucose via anthranilate. These results are a starting point to further optimize environmentally-compatible production capacity for catechol and derived compounds.
<title>Optical performance of the Grating Light Valve technology</title> (1999)

The objective of this paper is to detail the grating light valve technology and demonstrate its flexibility in attaining high performance in a variety of optical systems and applications, concentrating particularly on its application toward projection display systems. The GLV technology represent a unique approach to light modulation and offers remarkable performance in terms of contrast, efficiency, switching speed, and cost. The electro- mechanical response of the GLV device can be tuned through various design and operational modes to deliver desired performance for a given application. The design and fabrication of a linear array module of 1,088 GLV pixels is described. This module enables a scanned linear GLV architecture for HDTV projection products. The flexibility of the GLV technology and the scanned linear GLV architecture can support line sequential and frame sequential color, as well as 3-valve color systems. System level optical design either include embedded scanners to emulate 2D film source planes or external scanner elements for greater system simplicity. Results with actual projection display system yield unparalleled on-screen performance, having uniformity greater than 99 percent corner-to-corner, high contrast, 10-bits of grayscale per color, and no visible pixel boundaries.
Validity of the parenteral magnesium load test for mature mammals. (1989)

Because of the difficulty in accurately assessing the Mg status of a hospitalized patient, the parenteral Mg retention test may be a valuable diagnostic tool. In former studies in animal models, this test has reliably identified adult rats fed two extremes of dietary Mg, but we found no tests of intermediate levels of Mg in adult animals. As a means of assessing the validity of the parenteral Mg retention tests in adult rats, the present study was conducted to learn (1) the relationship between Mg in plasma and bone in adult mammals that had optimal nutrition at the onset of the experiment, and (2) the relationship between Mg retention and Mg in plasma and bone in those animals. Animals were fed five levels of dietary Mg from 0 to 150 mg/100 g purified diet. Parenteral Mg retention tests were conducted after 2 weeks of dietary treatment, and 18 h after the completion of the tests, plasma and bone were analyzed for Mg. We found that plasma and femur levels of Mg varied linearly under conditions of Mg deficit, indicated by a high retention of the Mg load. As the deficit diminished, both plasma and femur levels approached a limit and the slope of the curve tends to zero. Mg retention and plasma or femur Mg level was approximated by a negative exponential curve. Stated another way, the logarithmic values of Mg retention decreased approximately linearly with increase in bone or plasma Mg. It was concluded that the parenteral Mg retention test is a valid test to evaluate Mg deficiency and to identify Mg sufficiency in adult mammals.
Parametric design of power-split HEV drive train (2009)

In this paper, a design methodology is introduced for determining the major system parameters of a power-split hybrid vehicle drive train. The system parameters mainly includes the engine power rating, transmission gear ratios, alternator power and proper speed-torque characteristics, traction motor power rating and battery power and energy capacities. The design target is ensuring the vehicle to meet the specified vehicle performance, such as speed and acceleration, and at the same time, operate the engine within an expected speed range and torque range.
A multi-layer area routing algorithm with optimized pin mapping strategy (2003)

In this paper, we present a new multi-layer area routing algorithm with optimized pin mapping strategy. The algorithm can handle large routing problems, in which terminals and obstacles arbitrarily located in the routing area. An optimized pin mapping strategy is proposed in this paper as a pre-process before routing. We use the best matching of a bipartite graph to solve the pin-mapping problem. We tested our algorithm on a set of benchmark examples and compared our routing results with the results without pin-optimized mapping. Experiment results show that, the algorithm efficiently shortens the total wire length greatly and increases the routing completion rate.
Triassic sedimentation and palaeogeography in Central Skye (1975)

Synopsis The Triassic sediments are described including a number of new localities which extend the known area of cover. A new map shows the Mesozoic/Palaeozoic boundary at Camas Malag on Loch Slapin, where the basal Mesozoic sediments provide some critical evidence about the position of the latest Triassic and/or earliest Jurassic upland areas in central Skye. Lateral and vertical variation in the Triassic facies, pebble size and composition of the conglomerates are plotted and show that there were two distinct physiographic environments: an alluvial fan system which drained local Palaeozoic and Torridonian areas between Beinn an Dubhaich and Ben Suardal, and dispersed eastwards. a floodplain system which drained a metamorphic region north-east of Broadford and dispersed south-westwards. Their age relationship is uncertain, but local evidence suggests that they may have been penecontemporaneous. The succession is seen to represent the youngest and the most easterly Triassic sedimentation in the Minch region. Regional thickness changes suggest that the Minch basin-complex enlarged eastwards, towards the Scottish mainland, through the Triassic Period.
Chemical Mutagenesis of an Emissive RNA Alphabet (2015)

An evolved fluorescent ribonucleoside alphabet comprising isomorphic purine ((tz)A, (tz)G) and pyrimidine ((tz)U, (tz)C) analogues, all derived from isothiazolo[4,3-d]pyrimidine as a common heterocyclic core, is described. Structural and biochemical analyses illustrate that the nucleosides, particularly the C-nucleosidic purine analogues, are faithful isomorphic and isofunctional surrogates of their natural counterparts and show improved features when compared to an RNA alphabet derived from thieno[3,4-d]-pyrimidine. The restoration of the nitrogen in a position equivalent to the purines' N7 leads to "isofunctional" behavior, as illustrated by the ability of adenosine deaminase to deaminate (tz)A as effectively as adenosine, the native substrate.
Design and 3D printing of a stainless steel reactor for continuous difluoromethylations using fluoroform (2017)

The design and 3D printing of a stainless steel reactor for reaction with a gaseous reagent is described.
Condición física, percepción subjetiva del esfuerzo y rendimiento académico en educación primaria (2019)

Existen estudios que han conseguido establecer una relación entre el rendimiento cognitivo y académico de los alumnos y sus niveles de condición física. Los objetivos de esta investigación son estudiar la correlación existente entre el nivel de condición física y la composición corporal con el rendimiento académico, así como analizar la correlación entre dichas variables y la percepción subjetiva del esfuerzo (RPE) por género en alumnos de Educación Primaria. Un total de 69 alumnos de Educación Primaria participaron en el estudio (edad= 12,33±0,47 años, 55,9% niñas y 44,1% niños). La condición física y la composición corporal se evaluaron siguiendo los test de la ALPHA Fitness test Battery para niños y adolescentes. El rendimiento académico se estudió mediante el promedio de notas de las asignaturas instrumentales de Educación Primaria. La RPE se registró en las clases de EF y actividades físicas escolares mediante el uso de la escala RPE de Borg (0-10). Se establecieron correlaciones significativas entre la RPE y el peso de los chicos (r= 0,406, p= 0.02) y las chicas (r= 0,643, p< 0.001) y también con respecto al IMC, chicos (r= 0,508, p= 0.004), chicas (r= 0,621, p< 0.001). Existió una correlación negativa significativa entre el test de la Course Navette y la RPE en el caso de los niños (r= -0,633, p< 0.001) y de las niñas (r= -0,452, p= 0.004). La RPE se correlacionó significativamente con los palieres del test de Course Navette (correlación inversa) y las variables antropométricas, como son el peso y el IMC.
Eccentric Loading of Fluorogen with Aggregation‐Induced Emission in PLGA Matrix Increases Nanoparticle Fluorescence Quantum Yield for Targeted Cellular Imaging (2013)

A simple strategy is developed to prepare eccentrically or homogeneously loaded nanoparticles (NPs) using poly (DL-lactide-co-glycolide) (PLGA) as the encapsulation matrix in the presence of different amounts of polyvinyl alcohol (PVA) as the emulsifier. Using 2,3-bis(4-(phenyl(4-(1,2,2-triphenylvinyl)-phenyl)amino)-phenyl)-fumaronitrile (TPETPAFN), a fluorogen with aggregation-induced emission (AIE) characteristics, as an example, the eccentrically loaded PLGA NPs show increased fluorescence quantum yields (QYs) as compared to the homogeneously loaded ones. Field emission transmission electron microscopy and fluorescence lifetime measurements reveal that the higher QY of the eccentrically loaded NPs is due to the more compact aggregation of AIE fluorogens that restricts intramolecular rotations of phenyl rings, which is able to more effectively block the non-radiative decay pathways. The eccentrically loaded NPs show far red/near infrared emission with a high fluorescence QY of 34% in aqueous media. In addition, by using poly([lactide-co-glycolide]-b-folate [ethylene glycol]) (PLGA-PEG-folate) as the co-encapsulation matrix, the obtained NPs are born with surface folic acid groups, which are successfully applied for targeted cellular imaging with good photostability and low cytotoxicity. Moreover, the developed strategy is also demonstrated for inorganic-component eccentrically or homogeneously loaded PLGA NPs, which facilitates the synthesis of polymer NPs with controlled internal architectures.
Review of hardware-in-the-loop simulation and its prospects in the automotive area (2006)

<i>Hardware-in-the-loop (HIL) simulation</i> is rapidly evolving from a control prototyping tool to a system modeling, simulation, and synthesis paradigm synergistically combining many advantages of both physical and virtual prototyping. This paper provides a brief overview of the key enablers and numerous applications of HIL simulation, focusing on its metamorphosis from a control validation tool into a system development paradigm. It then describes a state-of-the art <i>engine-in-the-loop (EIL) simulation</i> facility that highlights the use of HIL simulation for the system-level experimental evaluation of powertrain interactions and development of strategies for clean and efficient propulsion. The facility comprises a real diesel engine coupled to accurate real-time driver, driveline, and vehicle models through a highly responsive dynamometer. This enables the verification of both performance and fuel economy predictions of different conventional and hybrid powertrains. Furthermore, the facility can both replicate the highly dynamic interactions occurring within a real powertrain and measure their influence on transient emissions and visual signature through state-of-the-art instruments. The viability of this facility for integrated powertrain system development is demonstrated through a case study exploring the development of advanced High Mobility Multipurpose Wheeled Vehicle (HMMWV) powertrains.
Fluorescent Labeling of Sugar-carboxylate with Water-soluble Carbodiimide (1992)

Journal Article Fluorescent Labeling of Sugar-carboxylate with Water-soluble Carbodiimide Get access Mikihiko Kobayashi, Mikihiko Kobayashi National Food Research Institute, Tsukuba 305, Japan Search for other works by this author on: Oxford Academic Google Scholar Eiji Ichishima Eiji Ichishima National Food Research Institute, Tsukuba 305, JapanDepartment of Agricultural Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Miyagi 981 , Japan Search for other works by this author on: Oxford Academic Google Scholar Bioscience, Biotechnology, and Biochemistry, Volume 56, Issue 2, 1 January 1992, Pages 186–189, https://doi.org/10.1271/bbb.56.186 Published: 01 January 1992 Article history Received: 28 May 1991 Published: 01 January 1992
The pathology of the feline model of mucopolysaccharidosis I. (1980)

Five cats with feline alpha-L-iduronidase-deficient mucopolysaccharidosis were studied. Membrane-bound cytoplasmic inclusions were present in central nervous system neurons, hepatocytes, chondrocytes, vascular and splenic smooth muscle cells, bone marrow leukocytes, and fibroblasts of the skin, eye, and cardiac valves. The lesions in these cats closely resemble those described in human patients with mucopolysaccharidosis I H (Hurler syndrome).
Thesaurus-based efficient example retrieval by generating retrieval queries from similarities (1994)

In example-based NLP, the problem of computational cost of example retrieval is severe, since the retrieval time increases in proportion to the number of examples in the database. This paper proposes a novel example retrieval method for avoiding full retrieval of examples. The proposed method has the following three features, 1) it generates retrieval queries from similarities, 2) efficient example retrieval through the tree structure of a thesaurus, 3) binary search along subsumption ordering of retrieval queries. Example retrieval time drastically decreases with the method.
Model compounds for R-state and T-state hemoglobins (1978)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModel compounds for R-state and T-state hemoglobinsJon Geibel, John Cannon, Dwane Campbell, and T. G. TraylorCite this: J. Am. Chem. Soc. 1978, 100, 11, 3575–3585Publication Date (Print):May 1, 1978Publication History Published online1 May 2002Published inissue 1 May 1978https://pubs.acs.org/doi/10.1021/ja00479a047https://doi.org/10.1021/ja00479a047research-articleACS PublicationsRequest reuse permissionsArticle Views461Altmetric-Citations78LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of Functional Residues in a 2-Hydroxymuconic Semialdehyde Hydrolase (1995)

The 2-hydroxymuconic semialdehyde hydrolase, XylF, of the Pseudomonas putida TOL plasmid-encoded pathway for the catabolism of toluene and xylenes, catalyzes one of the rarest types of enzyme reaction (EC 3.7.1.9), the hydrolysis of a carbon-carbon bond in its substrate, the ring-fission product of 3-alkyl-substituted catechols. In this study, amino acid sequence comparisons between XylF and other hydrolases, and analysis of the similarity between the predicted secondary structure of XylF and the known secondary structure of the haloalkane dehalogenase from Xanthobacter autotrophicus strain GJ10, led us to identify several conserved residues likely to have a functional role in the catalytic center of XylF. Three amino acids, Ser107, Asp228, and His256, were found to be arranged in a sequential order similar to that in alpha/beta hydrolase-fold enzymes. Investigations of the potential functional role of these and other residues through amino acid modification and in vitro site-directed mutagenesis experiments provided evidence in support of the hypothesis that XylF is a serine hydrolase of the alpha/beta hydrolase-fold family of enzymes, and pointed to the residues identified above as the catalytic triad of XylF. These studies also provided information on other conserved residues in XylF-related enzymes. Interestingly, the substitution of Phe by Met in position 108 of XylF created an enzyme with increased thermostability and altered substrate specificity.
Silicon Surface Contamination: Polishing and Cleaning (1973)

A Rutherford backscattering study using 2 MeV He+ ions has shown that the principal contaminants heavier than Si remaining on silica‐sol polished silicon surfaces are , and a heavy metal in the range . Preoxidation cleaning using and was found to be erratic in the ability to yield surfaces free of Ca and Cu and always left heavy metal contaminants. Cleaning with and solutions always removed all elements heavier than chlorine to below the level of detectability. Both cleaning treatments left . Breakdown voltage measurements indicated that junction quality was not predominantly controlled by surface impurities present after preoxidation cleaning, although the diode quality on cleaned wafers was much higher than on as‐polished wafers.
On the Mechanism of ITO Etching: The Specificity of Halogen Acids (1993)

Tin‐doped indium oxide films, prepared by dc magnetron sputtering, have been investigated with respect to their etching behavior in a large number of acids. The etch rate in acids other than the halogen acids is extremely low. The films dissolve at a rate convenient for practical use in concentrated aqueous solutions of halogen acids. Experiments in solutions diluted with solvents with a low dielectric constant show that the undissociated halogen acid is the active agent for the etching process. A mechanism is proposed to explain the observed results. Addition of or to solutions increases the etch rate substantially. Voltammograms and etching experiments under potentiostatic control show that this is a nonelectrochemical effect.
Aurora Kinases as Druggable Targets in Pediatric Leukemia: Heterogeneity in Target Modulation Activities and Cytotoxicity by Diverse Novel Therapeutic Agents (2014)

Leukemia is the most common pediatric malignancy, constituting more than 30% of all childhood cancers. Although cure rates have improved greatly, approximately one in five children relapse and poor survival rates post relapse remain a challenge. Given this, more effective and innovative therapeutic strategies are needed in order to improve prognosis. Aurora kinases, a family of serine/threonine kinases essential for the regulation of several mitotic processes, have been identified as potential targets for cancer therapeutics. Elevated expression of Aurora kinases has been demonstrated in several malignancies and is associated with aberrant mitotic activity, aneuploidy and alterations in chromosomal structure and genome instability. Based on this rationale, a number of small molecule inhibitors have been formulated and advanced to human studies in the recent past. A comparative analysis of these agents in cytotoxicity and target modulation analyses against a panel of leukemia cells provides novel insights into the unique mechanisms and codependent activity pathways involved in targeting Aurora kinases, constituting a distinctive preclinical experimental framework to identify appropriate agents and combinations in future clinical studies.
Barrier Enabled IO Stack for Flash Storage (2017)

This work is dedicated to eliminating the overhead of guaranteeing the storage order in modern IO stack. The existing block device adopts prohibitively expensive resort in ensuring the storage order among write requests: interleaving successive write requests with transfer and flush. Exploiting the cache barrier command for the Flash storage, we overhaul the IO scheduler, the dispatch module and the filesystem so that these layers are orchestrated to preserve the ordering condition imposed by the application can be delivered to the storage. Key ingredients of Barrier Enabled IO stack are Epoch based IO scheduling, Order Preserving Dispatch, and Dual Mode Journaling. Barrier enabled IO stack successfully eliminates the root cause of excessive overhead in enforcing the storage order. Dual Mode Journaling in BarrierFS dedicates the separate threads to effectively decouple the control plane and data plane of the journal commit. We implement Barrier Enabled IO Stack in server as well as in mobile platform. SQLite performance increases by 270% and 75%, in server and in smartphone, respectively. Relaxing the durability of a transaction, SQLite performance and MySQL performance increases as much as by 73X and by 43X, respectively, in server storage.