gebhart/patrepeval huggingface dataset:
Cross‐talk between epidermal growth factor receptor and protein kinase C during calcium‐induced differentiation of keratinocytes (2000)

The induction of epidermal differentiation by extracellular Ca2+ involves activation of both tyrosine kinase and protein kinase C (PKC) signaling cascades. To determine if the differentiation-dependent activation of tyrosine kinase signaling can influence the PKC pathway, we examined the tyrosine phosphorylation status of PKC isoforms in primary mouse keratinocytes stimulated to terminally differentiate with Ca2+. Elevation of extracellular Ca2+ induced tyrosine phosphorylation of PKC-delta, but not the other keratinocyte PKC isoforms (alpha, epsilon, eta, zeta). We have previously demonstrated that activation of the epidermal growth factor receptor (EGFR) pathway induces PKC-delta tyrosine phosphorylation in basal keratinocytes (Denning M F, Dlugosz A A, Threadgill D W, Magnuson T, Yuspa S H (1996) J Biol Chem 271: 5325-5331). When basal keratinocytes were stimulated to differentiate by Ca2+, the level of cell-associated transforming growth factor-alpha (TGF-alpha) increased 30-fold, while no increase in secreted TGF-alpha was detected. Furthermore, Ca2+-induced tyrosine phosphorylation of PKC-delta and phosphotyrosine-association of the receptor adapter protein Shc was diminished in EGFR -/- keratinocytes, suggesting that EGFR activation may occur during keratinocyte differentiation. Tyrosine phosphorylated PKC-delta was also detected in mouse epidermis, suggesting that this differentiation-associated signaling pathway is physiological. These results establish a requirement for the EGFR in Ca2+-induced tyrosine phosphorylation of PKC-delta, and document the production of cell-associated TGF-alpha in differentiated keratinocytes which may function independent of its usual mitogenic effects.
Influence of Ion Channel Modulation on in Vitro Interferon-γ Induced MHC Class I and II Expression on Macrophages (1995)

The in vitro effect of K+ channel blockers quinidine and verapamil, anion channel blocker SITS and K+ channel openers diazoxide, pinacidil, and BRL 38227 on interferon-gamma (IFN-gamma) induced MHC class I and II expression of Lewis rat peritoneal macrophages was investigated by cell ELISA assay. MHC class I expression was significantly enhanced by diazoxide at concentrations of 10(-5)M to 10(-6)M and by pinacidil and BRL 38227 at the concentration of 10(-6)M. MHC class II expression was enhanced by pinacidil and BRL 38227 at concentrations of 10(-5)M to 10(-6)M. The enhancing effect of pinacidil could be blocked by inhibitors of the protein kinases PKA and PKC suggesting that activation of both is required for optimum induction of MHC molecule expression. K+ and anion channel blockers were less active in modulation of MHC molecule expression. Verapamil had no influence, quinidine suppressed MHC class I expression at concentrations of 10(-4)M to 10(-5)M, and SITS suppressed MHC class I expression at the concentration of 10(-3)M. Since MHC class II expression is essential for efficient antigen presentation to T helper cells and MHC class I expression is required for target cell lysis by cytotoxic T cells, ion channel modulating drugs may be potential candidates for immunopharmacological intervention in inflammatory diseases.
The Application of a Microwave Concentrator/Biofilter Integrated System to Treat Paint Booth Emissions (2000)

Typical spray paint booth emissions have been shown to be readily treatable using biofilter treatment systems. However, if constant organic loading is not introduced to a biofilter, an effective microbial population may not be maintained. Typical spray paint booths provide a transient, unsteady-state organic load. Thus, the addition of a concentrator provided upstream of a biofilter can eliminate these unsteady loading conditions so that the biofilter microbial population can thrive. In cooperation with the Air Force Research Laboratory (AFRL/MLQ) and Tyndall Air Force Base (Panama City, FL), Envirogen (Lawrenceville, NJ) and CHA Corporation (Laramie, WY) have recently begun the operation of a pilot-scale integrated microwave concentrator/biofilter system for the treatment of solvents discharged from spray paint booth operations. In the first phase of the treatment process, a ventilated air stream (2,000 SCFM) containing the solvents methyl ethyl ketone, 2-pentanone, and toluene from spray painting operations is passed continuously through a granular activated carbon (GAC) moving-bed adsorber. The solvent- saturated GAC is regenerated via microwave energy in a separate regeneration vessel. The solvent vapors are removed from the regenerator by a small stream of nitrogen purge gas and transferred to storage tanks. On a continuous basis, solvent-laden nitrogen from the storage tanks is supplied to an Envirogen biofilter along with dilution air. Envirogen's biofilter is an air pollution control system in which contaminated air is passed through a bed of organic filter material containing a natural flora of microorganisms. The contaminants are degraded by the microbes on the filter bed into innocuous products of carbon dioxide and water. Critical findings at the pilot-scale, overall system performance, and the potential applicability at the full- scale system will be discussed at the conference.
Compaction mechanism and tablet strength of unlubricated and lubricated (silicified) microcrystalline cellulose (2004)

This paper describes the differences in compaction properties between microcrystalline cellulose (MCC) and microcrystalline cellulose co-processed with colloidal silicon dioxide (SMCC). The different compaction parameters are not only compared for the pure materials, but also for the lubricated powders with magnesium stearate. Neither magnesium stearate, nor colloidal silicon dioxide, facilitates extensively the densification of (silicified) microcrystalline cellulose during compaction. The difference in tablet relaxation of MCC and SMCC indicates a small negative effect of colloidal silicon dioxide on the interparticle bonding strength of unlubricated MCC. However, for lubricated MCC a larger increase in tablet relaxation at a high compression speed was found than for lubricated SMCC tablets. Accordingly, the decrease in tablet strength was larger for the MCC tablets than for the SMCC tablets when lubrication was applied. The examination of the tablet strengths of tablets compressed from physical mixtures of MCC with increasing concentrations of colloidal silicon dioxide proved the slightly negative influence of silicon dioxide on the tablet strength of unlubricated MCC tablets and the positive effect of colloidal silicon dioxide addition on the tensile strength of lubricated MCC tablets. Co-processing of MCC with colloidal silicon dioxide showed no extra contribution on the tablet strength of lubricated tablets above the physical mixtures. The interactions between the different materials were further supported by the interaction parameters based on partial solubility parameters.
Investigation of Inter-Cell Transmission Power Control Using Overload Indicator for Selected Users for Evolved UTRA Uplink (2009)

This paper proposes the use of inter-cell transmission power control (TPC) with overload indicator (OLI) signaling to user equipment (UE) in addition to intra-cell TPC for the Evolved UTRA uplink. In the proposed inter-cell OLI transmission method, a cell site (Node B) selects UEs offering high-level interferences to the cell site based on the measured path loss difference, and then, the cell site transmits the OLI signal to the selected UEs. The simulation results show that the inter-cell TPC improves both the average user throughput and cell-edge user throughput at 5% in the cumulative distribution function (CDF) curve, assuming the same sector throughput. For instance, when the sector throughput is 1Mbps using 1.08MHz bandwidth, the inter-cell TPC with the proposed UE-common OLI scheme increases the average user throughput and the 5%-cell edge user throughput by approximately 41% and 53%, respectively, compared to the case with intra-cell TPC only. Furthermore, when the inter-cell TPC with the proposed UE-individual OLI is employed, the corresponding average user throughput and the 5% user throughput are increased by approximately 87% and 94%, respectively.
A Luminescent Mixed-Lanthanide-Organic Framework Sensor for Decoding Different Volatile Organic Molecules (2014)

A flexible tripodal polyaromatic acid (4,4',4″-(((2,4,6-trimethylbenzene-1,3,5-triyl)-tris(methylene))-tris(oxy))tribenzoic acid, H3TCM) was used to adapt the coordination sites of lanthanide ions for the construction of microporous lanthanide-organic frameworks (LOFs) [LnTCM(H2O)2]·3DMF·H2O (Ln-TCM; Ln = La, Eu, and/or Tb). In these LOFs, the emission band of TCM matches well with the excitation energy of lanthanide ions (Eu(3+) and Tb(3+)) which results in high-efficient resonance energy transfer from TCM to lanthanide ions. Moreover, the mixed EuxTb1-x-TCM has tunable pores to adapt different induced-fit-type host-guest interactions which can modulate both the energy transfer efficiency from TCM to Ln(3+) ions and the energy allocation between Eu(3+) and Tb(3+) ions in the luminescence spectra. We demonstrate that the Eu(x)Tb(1-x)-TCM sensor has the capability of decoding different volatile organic molecules (VOMs) with a clearly differentiable and unique emission intensity ratio of (5)D0 → (7)F2 (Eu(3+), 614 nm) to (5)D4 → (7)F5 (Tb(3+), 545 nm) transitions for every different VOM. Compared with the traditional absolute emission intensity method, such a self-referencing emission intensity strategy has generated self-calibrating, highly differentiable, and very stable luminescent signals for decoding different VOMs from the unique Eu(x)Tb(1-x)-TCM platform, which has great potential for practical applications.
A Novel Mechanism of Retinoic Acid–Enhanced Interleukin-8 Gene Expression in Airway Epithelium (2000)

A 3- to 8-fold stimulation of interleukin (IL)-8 gene expression by all-trans-retinoic acid (ATRA) was demonstrated in primary cultures of human and monkey tracheobronchial epithelial cells and BEAS-2B serum-sensitive cell line. The effect of ATRA on IL-8 gene expression is dose- and time-dependent. Using cycloheximide, it was observed that new protein synthesis was required for the stimulation. ATRA had no effect on IL-8 messenger RNA stability. A difference in nuclear run-on activity suggests that a transcriptional mechanism is involved in ATRA-enhanced IL-8 gene expression. Promoter-reporter gene transfection studies demonstrated ATRA enhanced IL-8 promoter activity, especially when cells were cotransfected with retinoic acid nuclear receptor-alpha expression vector. Deletion and site-directed mutagenesis analysis revealed the involvement of nuclear factor (NF)-kappaB binding site of the IL-8 gene in ATRA-enhanced promoter activity. Electrophoretic mobility shift assay (EMSA) demonstrated that ATRA enhanced DNA-NF-kappaB complex formation, especially with the p65 subunit. Western blot analysis demonstrated that ATRA did not enhance the protein amount of both the p50 and the p65 subunits in the nuclei. Because ATRA also enhances thioredoxin (TRX) gene expression, the effect of TRX on IL-8 gene expression was examined. IL-8 promoter activity was enhanced in transfected cells by the addition of TRX protein. Treatment of nuclear extracts with TRX also enhanced DNA- NF-kappaB complex formation as observed by EMSA, particularly the p65 subunit. Taking these data together, a novel mechanism is proposed in which ATRA activates promoter activity of IL-8 gene through TRX-dependent NF-kappaB activation.
Purification and characterization of epidermal growth factor receptor/protein kinase from normal mouse liver. (1982)

We have purified the epidermal growth factor (EGF) receptor/protein kinase from the livers of normal mice by affinity chromatography. The biochemical properties of the liver receptor are very similar to those of the EGF receptor previously prepared from the human tumor cell line A-431 [Cohen, S., Ushiro, H., Stoscheck, C. & Chinkers, M. (1982) J. Biol. Chem. 257, 1523-1531]. The liver receptor for EGF is a glycoprotein of Mr 170,000. It binds 125I-labeled EGF and possesses an EGF-stimulable protein kinase activity specific for tyrosine residues. Both autophosphorylation and kinase activity toward exogenous substrates are demonstrable. The EGF receptor purified from normal mouse liver is antigenically related to the receptor purified from human A-431 cells.
Estimating nonnegative matrix model activations with deep neural networks to increase perceptual speech quality (2015)

As a means of speech separation, time-frequency masking applies a gain function to the time-frequency representation of noisy speech. On the other hand, nonnegative matrix factorization (NMF) addresses separation by linearly combining basis vectors from speech and noise models to approximate noisy speech. This paper presents an approach for improving the perceptual quality of speech separated from background noise at low signal-to-noise ratios. An ideal ratio mask is estimated, which separates speech from noise with reasonable sound quality. A deep neural network then approximates clean speech by estimating activation weights from the ratio-masked speech, where the weights linearly combine elements from a NMF speech model. Systematic comparisons using objective metrics, including the perceptual evaluation of speech quality, show that the proposed algorithm achieves higher speech quality than related masking and NMF methods. In addition, a listening test was performed and its results show that the output of the proposed algorithm is preferred over the comparison systems in terms of speech quality.
Sonography of the Sole of the Foot (1986)

The hypothesis that loss of tissue mass in the foot contributes to foot ulceration in diabetics has never been quantitated. We developed normal criteria for the thickness of the sole of the foot at the heel, and the five metatarsal heads of both feet, using high-resolution ultrasound (10 MHz). We studied 24 normal patients ranging in weight from 125 to 250 lbs. We examined the soles of the feet of 38 diabetics without foot ulcers and 11 diabetics with foot ulcers or a history of foot ulcers who were in the same weight range as the normals. Of statistical significance, the heel thickness in controls was greater than that of the diabetics, which in turn was greater than that of the diabetics with foot ulcers. The thickness of the sole over the first and second metatarsals was also greater in the controls compared with diabetics. We conclude that high-resolution ultrasound is an effective determinant of the thickness of the sole of the foot and that diabetics have variations from the norm in the heel and at the first and second metatarsal heads.
Two apical multidrug transporters, P-gp and MRP2, are differently altered in chronic renal failure (2001)

Tubular function is altered in chronic renal failure (CRF). Whether drug secretion by renal tubules is modified in CRF is questioned because of frequent accumulation of various toxins in CRF. This function mainly involves ATP-dependent drug transporters, particularly P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) 2, both present in apical membrane of epithelial cells. The present study was aimed at determining the changes in P-gp and MRP2 expression induced by experimental CRF in kidney and liver. The relationship between MRP2 and glutathione metabolism changes was examined because MRP2 transports GSSG and glutathione conjugates. Rats underwent either 80% subtotal nephrectomy (Nx) or sham operation, and determinations were performed 3 and 6 wk later. CRF induced a 70--200% rise in protein and mRNA expression of MRP2 after 3 and 6 wk post-Nx in remnant kidney and after 6 wk in liver. However, P-gp expression was unchanged by CRF. Relative to whole kidney mass, total MRP2 levels decreased by only 27% in Nx rats whereas total P-gp levels were reduced by 60%. Renal GSSG and total glutathione levels were increased by 30% in Nx rats, but glutathione-S-transferase (GST) activity was normal; liver GSSG levels and GST activity were reduced in Nx rats. In conclusion, CRF resulted in specific overexpression of MRP2 in kidney and liver. This could be an adaptative response to some elevated circulating toxins. The later MRP2 induction and different glutathione changes in liver compared with kidney suggest different mechanisms for MRP2 induction and/or action in these two tissues.
Paralytic Shellfish Poison. VI. A Procedure for the Isolation and Purification of the Poison from Toxic Clam and Mussel Tissues (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTParalytic Shellfish Poison. VI. A Procedure for the Isolation and Purification of the Poison from Toxic Clam and Mussel TissuesEdward J. Schantz, James D. Mold, D. Warren Stanger, John Shavel, Frank J. Riel, John P. Bowden, Joseph M. Lynch, Ruth Savage Wyler, Byron Riegel, and Hermann SommerCite this: J. Am. Chem. Soc. 1957, 79, 19, 5230–5235Publication Date (Print):October 1, 1957Publication History Published online1 May 2002Published inissue 1 October 1957https://pubs.acs.org/doi/10.1021/ja01576a044https://doi.org/10.1021/ja01576a044research-articleACS PublicationsRequest reuse permissionsArticle Views588Altmetric-Citations180LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Whole-cell-analysis of live cardiomyocytes using wide-field interferometric phase microscopy (2010)

We apply wide-field interferometric microscopy techniques to acquire quantitative phase profiles of ventricular cardiomyocytes in vitro during their rapid contraction with high temporal and spatial resolution. The whole-cell phase profiles are analyzed to yield valuable quantitative parameters characterizing the cell dynamics, without the need to decouple thickness from refractive index differences. Our experimental results verify that these new parameters can be used with wide field interferometric microscopy to discriminate the modulation of cardiomyocyte contraction dynamics due to temperature variation. To demonstrate the necessity of the proposed numerical analysis for cardiomyocytes, we present confocal dual-fluorescence-channel microscopy results which show that the rapid motion of the cell organelles during contraction preclude assuming a homogenous refractive index over the entire cell contents, or using multiple-exposure or scanning microscopy.
Variable birefringent beam splitter (1981)

A sapphire disk is used as a variable birefringent beam splitter for the linearly polarized pump beam in an oscillator-amplifier dye laser system. Rotation of the disk varies the extent of retardation. The induced elliptical polarization results in a variable back surface reflectance between approximately 0.02-0.15 for the green spectral region. Reflectances are measured on two disks with different thicknesses and compared with an approximate model describing the reflectance as a function of angle of rotation of the disk.
Design and Application Criteria for Connecting Couplings (1986)

A classification of couplings as rigid, misalignment-compensating, torsionally flexible, and combination purpose is proposed. Selection criteria for two basic subclasses of misalignment-compensating couplings are derived, some standard designs are analyzed, and modifications of Oldham and gear couplings in which compensatory motion is accommodated by internal shear in thin elastomeric layers instead of reciprocal sliding are described. The new designs demonstrate very high efficiency and exert substantially reduced forces on the connected shafts. Factors determining the influence of torsionally flexible couplings on transmission dynamics are formulated as reduction of torsional stiffness, enhancement of damping, modification of nonlinearity, and inertia distribution. Compensation properties of combination purpose couplings are investigated analytically and a “design index” is introduced. A comparison of important characteristics of some commercially available types of combination purpose couplings is performed, to facilitate an intelligent comparison and selection of various coupling types. A line of approach for the improvement of torsionally flexible / combination purpose couplings by using highly nonlinear elastomeric elements is suggested.
Borges y el universo de los signos (1974)

A 73-year-old woman with a history of paroxysmal atrial fibrillation, sinus node dysfunction, bilateral breast cancer, and extensive chest radiation developed progressive edema, dyspnea, and recurrent pleural effusions soon after single-chamber pacemaker implantation. Thoracentesis yielded a diagnosis of chylothorax, and progressive refractory anasarca developed. A computed tomography angiogram suggested obstruction of the superior vena cava and left subclavian vein despite outpatient therapeutic anticoagulation. Autopsy confirmed venous thrombosis, along with mediastinal fibrosis. The presumed etiology of the chylothorax and anasarca was obstruction of the atretic central venous structures following pacemaker implantation, critically impairing the already tenuous venous and lymphatic drainage. (PACE 2010; 520-524).
X-ray Bursting Neutron Stars (1987)

The maximum peak luminosity of the X-ray bursts from a burster is most likely interpreted as the Eddington luminosity of a helium-rich envelope surrounding a neutron star. If this interpretation is true, we can obtain a relation between the mass and the radius of the neutron star in terms of the maximum effective temperature of bursts. On the other hand, the most naive understanding of the origin of the 4.1 keV absorption line often detected in X-ray burst spectra gives us another relation of the neutron star mass with its radius. By solving two simultaneous equations, we can determine the values of the mass and the radius of the neutron star, respectively. However, the result is critical to every neutron star model currently considered. The persistent emissions from X-ray bursters are also discussed.
Power-cell Switching-Cycle Capacitor Voltage Control for the Modular Multilevel Converters (2014)

This paper presents a power-cell Switching-Cycle Capacitor Voltage Control (SCCVC) approach to control the capacitor voltage ripple for the Modular Multilevel Converters (MMC). The capacitor voltage will be following the reference in one switching cycle by taking advantage of the degree of freedom in the control, the circulating current. The capacitor voltages resonate with the inductors in the converter circuit when the capacitors are inserted into the converter arm, and can reach the reference voltage at the end of the insertion time interval by proper control of the initial arm current. The SCCVC decouples the capacitor voltage ripple magnitude apart from the line frequency to certain extent, which not only provides a significant reduction of the capacitance value in designing the MMC parameters, but also enable the MMC to deliver very low frequency or even DC line currents. The control principle of the SCCVC and the resonance behavior between the capacitors and inductors are shown in this paper and finally the simulation results validate the control feasibility.