gebhart/patrepeval huggingface dataset:
Ribonuclease H-mediated inhibition of translation and reverse transcription by antisense oligodeoxynucleotides (1992)

Conference Article| November 01 1992 Ribonuclease H-mediated inhibition of translation and reverse transcription by antisense oligodeoxynucleotides Claudine Boiziau; Claudine Boiziau 1Laboratoire de Biophysique Moléculaire, INSERM CJF 90–13, Université de Bordeaux II, 33076 Bordeaux Cedex, France Search for other works by this author on: This Site PubMed Google Scholar Béatrice Larrouy; Béatrice Larrouy 1Laboratoire de Biophysique Moléculaire, INSERM CJF 90–13, Université de Bordeaux II, 33076 Bordeaux Cedex, France Search for other works by this author on: This Site PubMed Google Scholar Serge Moreau; Serge Moreau 1Laboratoire de Biophysique Moléculaire, INSERM CJF 90–13, Université de Bordeaux II, 33076 Bordeaux Cedex, France Search for other works by this author on: This Site PubMed Google Scholar Christian Cazenave; Christian Cazenave 1Laboratoire de Biophysique Moléculaire, INSERM CJF 90–13, Université de Bordeaux II, 33076 Bordeaux Cedex, France Search for other works by this author on: This Site PubMed Google Scholar David Shire; David Shire *Sanofi Elf BioRecherches, 31676 Labège, France Search for other works by this author on: This Site PubMed Google Scholar Jean-Jacques Toulmé Jean-Jacques Toulmé † 1Laboratoire de Biophysique Moléculaire, INSERM CJF 90–13, Université de Bordeaux II, 33076 Bordeaux Cedex, France †Correspondence to Dr. Jean-Jacques Toulmé, Laboratoire de Biophysique Moléculaire, Université de Bordeaux II, Bât. 3a, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1992) 20 (4): 764–767. https://doi.org/10.1042/bst0200764 Article history Received: July 22 1992 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share MailTo Twitter LinkedIn Cite Icon Cite Get Permissions Citation Claudine Boiziau, Béatrice Larrouy, Serge Moreau, Christian Cazenave, David Shire, Jean-Jacques Toulmé; Ribonuclease H-mediated inhibition of translation and reverse transcription by antisense oligodeoxynucleotides. Biochem Soc Trans 1 November 1992; 20 (4): 764–767. doi: https://doi.org/10.1042/bst0200764 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: AMV, avian myeloblastosis virus, PS, phosphorothioate, PO, phosphodiester, MP, methylphosphonate, HIV, human immunodeficiency virus This content is only available as a PDF. © 1992 Biochemical Society1992 Article PDF first page preview Close Modal You do not currently have access to this content.
PAIN (1981)

Assistant Professors, Clinical Pharmacy, Philadelphia College of Pharmacy and Science, Philadelphia, Pennsylvania RENEE CANTWELL, RN, BS, Critical Care Nursing MAUREEN ROGERS, RN, BSN, Nursing Education ROSE HOLLIS, RN, MEd, Nursing Education Instructors, Veterans Administration Medical Center, Philadelphia, Pennsylvania
Thermus aquaticus YT - 1 의 내열성 프로테아제 aqualysin I 의 구조와 특징 (1988)

Aqualysin I is an alkaline serine protease which is secretet into the culture medium by Thermos aquaticus YT-1, an extreme thermophile. Aqualysin I was purified, and its partial amino acid sequence was determined. The gene encoding aqualysin I was cloned into E. coli using synthetic oligodeoxyribonucleotides as hybridization probes. The nucleotide sequence of the cloned DNA was determined. The primary structure of aqualysin I, deduced from the nucleotide sequence, agreed with the determid amino acid sequences, including the NH₂- and COOH terminal sequence of the tryptides derived from aqualysin I. Aqualysin I comprised 281 amino acid residues and its molecular mass was determined to 1)e 28350. On alignment of the whole amino acid sequence, aqualysin I showed high sequence homology with the subtilisin type serine protease, and 43% identity with proteinase K, 37-30% with subtilisins and 34% with thermitase. Extremely high sequence identity was observed in the regions containing the active-site residues, corresponding to Asp32, His64 and Ser221 of subtilisin BPN`. Aqualysin I contains two disulfide bonds, Cys67-Cys99 and Cys163-Cysl94, and these disulfide bonds seem to contribute to the heat stability of the enzyme. The determined positions of the twe disulfide bonds of aqualysin I agreed with those predicted previously on the basis of computer graphics of the crystallographic data for subtilisin BPN`. Therefore, these findings sugests that the three-dimensional structure of aqualysin I is similar to that of subtilisin BPN` Aqualysin I is produced as a lage precursor, which contains NH₂- and COOH- terminal portions besides the mature protease sequence.
Increased expression of c‐Ski as a co‐repressor in transforming growth factor‐β signaling correlates with progression of esophageal squamous cell carcinoma (2004)

Transforming growth factor-beta (TGF-beta) regulates cell growth inhibition, and inactivation of the TGF-beta signaling pathway contributes to tumor development. In our previous study, altered expression of TGF-beta, TGF-beta-specific receptors and Smad4 was shown to correlate with tumor progression in esophageal squamous cell carcinoma (SCC). These components, however, were maintained normally in some patients with esophageal SCC. In our study, the mechanism by which aggressive esophageal SCC maintains these components was investigated, with particular emphasis on the participation of c-Ski and SnoN as transcriptional co-repressors in TGF-beta signaling. Immunohistochemistry for c-Ski and SnoN was carried out on surgical specimens obtained from 80 patients with esophageal SCC. The expression of c-Ski and SnoN was also studied in 6 established cell lines derived from esophageal SCC and compared to an immortalized human esophageal cell line by Western blotting. High levels of expression of c-Ski, detected immunohistologically, were found to correlate with depth of invasion (p = 0.0080) and pathologic stage (p = 0.0447). There was, however, no significant correlation between expression of SnoN and clinicopathologic characteristics. A significant correlation between c-Ski and TGF-beta expression was observed. Moreover, in patients with TGF-beta negative expression, the survival rates of patients with c-Ski positive expression were significantly lower than those of patients with c-Ski negative expression (p = 0.0486). c-Ski was expressed at a high level in 5 of 6 cell lines derived from esophageal SCC compared to immortalized esophageal keratinocytes. Furthermore, the cyclin-dependent kinase (CDK) inhibitor, p21 that was up-regulated by TGF-beta signaling was expressed at a low level in the 5 cell lines. The expression of c-Ski protein as a transcriptional co-repressor in TGF-beta signaling seems to be correlated with tumor progression of esophageal SCC.
A multipole analysis of a dielectric loaded coaxial rectangular waveguide (1992)

A multipole analysis of a coaxial rectangular waveguide whose inner conductor is circular is made in order to determine the TE and TM modes of the system. The analysis is based on using multipole (dipole, quadrupole etc.) electric and magnetic current sources to generate field solutions in the waveguide. These solutions are used to match the electromagnetic boundary condition in a homogeneous coaxial rectangular waveguide and to determine the TE and TM eigenvalues of the waveguide system. Eigenvalue results are compared with results of the generalized spectral domain method and to eigenvalue results for a ridged waveguide. Propagation in a coaxial rectangular waveguide is also studied when the coaxial rectangular waveguide is loaded with lossy inhomogeneous dielectric material. A variational formula is used to relate the TEM, TE, and TM modes of an empty coaxial rectangular waveguide to the propagation in the loaded inhomogeneous dielectric waveguide.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Unexpected similarity between the cytosolic West Nile virus NS3 and the secretory furin-like serine proteinases (2005)

Many viral proteins undergo proteolytic processing events that are required for virus infection and virion assembly. In this issue of Biochemical Journal, Strongin and co-workers report that the NS3 protease from West Nile virus unexpectedly cleaves certain substrates at pairs of basic residues, a specificity that resembles that of the furin-like PCs (proprotein convertases). This led to the demonstration that furin/PC inhibitors containing poly(D-arginine) are also potent inhibitors of NS3, and that anthrax toxin protective antigen and myelin basic protein are potential NS3 substrates. Structural modelling based on Dengue virus NS3 provided a possible rationale for the observed cleavage specificity of West Nile virus NS3.
THE MECHANISM OF CARBOHYDRATE OXIDATION. VII. THE ACTION OF POTASSIUM HYDROXIDE ON DIHYDROXY ACETONE (1928)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE MECHANISM OF CARBOHYDRATE OXIDATION. VII. THE ACTION OF POTASSIUM HYDROXIDE ON DIHYDROXY ACETONEWilliam Lloyd Evans and William Robert CornthwaiteCite this: J. Am. Chem. Soc. 1928, 50, 2, 486–492Publication Date (Print):February 1, 1928Publication History Published online1 May 2002Published inissue 1 February 1928https://doi.org/10.1021/ja01389a036RIGHTS & PERMISSIONSArticle Views116Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (377 KB) Get e-Alerts Get e-Alerts
Kinetics of Bacterial Growth: Relationship between Population Density and Specific Growth Rate of Continuous Cultures (1959)

SUMMARY: Results from studies of continuous cultures of Aerobacter aerogenes growing in chemically denned media indicate that specific growth rate (R) is a function of population density (P) as well as the concentration of the limiting nutrient (S). From these observations, and those of others, the following model for bacterial growth is derived: R = u m S B P + S where um and B are growth parameters that are constants under defined conditions. This model is believed to have general applicability and to account for bacterial growth in both batch and continuous cultures.
Nucleophilicity towards a saturated carbon atom: rate constants for the aminolysis of methyl 4-nitrobenzenesulfonate in aqueous solution. A comparison of the n and N + parameters for amine nucleophilicity (1994)

Second-order rate constants (kNu) have been measured in aqueous solution (I= 0.1 mol dm –3, 25 °C) for the SN2 reactions of methyl 4-nitrobenzenesulfonate with ammonia, 41 primary amines, 20 secondary amines. 29 tertiary amines and 7 anionic nucleophiles. For the aminolysis reactions, Brønsted-type correlations of nucleophilicity with basicity require the classification of all amines in terms of strictly defined structural classes with βnuc in the range 0.15–0.39. Swain–Scott plots indicate that simple amines, water and other light-atom nucleophiles (hydroxide, azide and cyanide, anions) are five times more reactive than heavy-atom nucleophiles (thiosulfate, thiocyanate, iodide and bromide ions). For amine nucleophiles there is a close linear correlation (of slope 0.44, and including both primary and secondary amines) between log kNu for the aminolysis of methyl 4-nitrobenzenesulfonate and log kNu for amine addition to the 1-methyl-4-vinylpyridinium cation. This correlation demonstrates a close linear relationship between the Swain–Scott n parameter and Ritchie's N+parameter for amine nucleophiles in aqueous solution (N+= 2.1n– 4.3).
Further Studies of Iodination in Liquid Ammonia (1934)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFurther Studies of Iodination in Liquid AmmoniaThomas H. Vaughn and J. A. NieuwlandCite this: J. Am. Chem. Soc. 1934, 56, 5, 1207–1209Publication Date (Print):May 1, 1934Publication History Published online1 May 2002Published inissue 1 May 1934https://pubs.acs.org/doi/10.1021/ja01320a065https://doi.org/10.1021/ja01320a065research-articleACS PublicationsRequest reuse permissionsArticle Views183Altmetric-Citations24LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Through-Silicon-Via-Based Decoupling Capacitor Stacked Chip in 3-D-ICs (2013)

In this paper, a new decoupling capacitor stacked chip (DCSC) based on extra decoupling capacitors and through-silicon-vias (TSVs) is proposed to overcome the narrow-bandwidth limitation of the conventional decoupling capacitor solutions in three-dimensional-integrated circuits (3-D-ICs), as exhibited by expensive on-chip metal-oxide-semiconductor (MOS) decoupling capacitors and inductive off-chip discrete decoupling capacitors. In particular, in comparison to the on-chip decoupling solutions, such as MOS, metal-insulator-metal and deep trench capacitors, the proposed TSV-based DCSC represents several advantages, such as small leakage currents, large capacitances ranging from tens of nF to a few μF, low equivalent series inductance (ESL) with tens of pH, and high flexibility in TSV arrangements. The proposed TSV-based DCSC can be applied by mounting decoupling capacitors, such as Si-based MOS capacitors and discrete capacitors, on the backside of a chip and connecting the capacitors to the on-chip power delivery network (PDN) through TSVs. To demonstrate the performance of the proposed DCSC structure, a segmentation method was applied to compare the PDN impedance (Z11) of the TSV-based DCSC with those of the well-known conventional decoupling capacitor methods. The TSV-based DCSC was found to exhibit the advantages of both low on-chip level ESL (under several tens of pH) and high off-chip level capacitance (up to several μF). Additionally, the PDN impedance properties of the TSV-based DCSC were analyzed with respect to the variations in the number of power/ground TSV pairs, on-chip PDN size, and capacitance values of the stacked off-chip discrete decoupling capacitors using the segmentation method.
The Common Vaccine Adjuvant Aluminum Hydroxide Up-Regulates Accessory Properties of Human Monocytes via an Interleukin-4-Dependent Mechanism (2001)

Aluminum adjuvants are widely used in human vaccines based on their ability to enhance antibody production. However, the mechanisms underlying these effects remain unknown. In the present study we assessed the direct in vitro effect of aluminum hydroxide on human peripheral blood monocytes, specifically with regard to its impact on the phenotype and functional properties of this cell population. Our results revealed significant changes in the accessory properties of monocytes following short-term exposure of cultured cells to aluminum hydroxide. Thus, flow cytometry analyses showed an increase in the expression of major histocompatibility complex (MHC) class II, CD40, CD54, CD58, CD83, and CD86 molecules on the monocytes. In addition, many cells in the cultures containing aluminum hydroxide acquired typical dendritic morphology. Increased synthesis of interleukin-4 (IL-4) mRNA, but not gamma interferon mRNA, was also noted after exposure to aluminum hydroxide. The increase in cell surface expression of MHC class II did not occur in the presence of neutralizing IL-4 antibody or in cultures of highly purified monocytes or CD4-depleted mononuclear cells. Our findings suggest that aluminum hydroxide directly stimulates monocytes to produce proinflammatory cytokines activating T cells. Activated Th2 cells release IL-4, which in turn can induce an increase in the expression of MHC class II molecules on monocytes. The increase in the expression of antigen-presenting and costimulatory molecules leads to enhanced accessory functions of monocytes. These properties of aluminum hydroxide observed in vitro may explain its potent in vivo adjuvant effect.
gamma-Glutamyltranspeptidase from Escherichia coli K-12: formation and localization (1986)

Escherichia coli cells showed maximum activity of gamma-glutamyltranspeptidase (EC 2.3.2.2) when they were grown at 20 degrees C, 14% of maximum activity at 37 degrees C, and none at 43 degrees C. The enzyme activity of intact cells grown at 20 degrees C was stably maintained after the temperature was changed to 45 degrees C. The activity increased during the exponential phase, and maximum activity was found at stationary phase. Its intracellular localization in the periplasmic space was confirmed.
Frizzled-7 as a Potential Therapeutic Target in Colorectal Cancer (2008)

We investigated whether one of the Wnt receptors, frizzled-7 (FZD7), functions in the canonical Wnt signaling pathway of colorectal cancer (CRC) cells harboring an APC or CTNNB1 mutation and may be a potential therapeutic target for sporadic CRCs. The expression level of FZD gene family members in colon cancer cells and primary CRC tissues were determined by real-time PCR. Activation of the Wnt signaling pathway was evaluated by TOPflash assay. The expression level of Wnt target genes was determined by real-time polymerase chain reaction and/or Western blot analysis. Cell growth and cell invasion were assessed by MTS and matrigel assays, respectively. Among 10 FZD gene family members, FZD7 mRNA was predominantly expressed in six colon cancer cell lines with APC or CTNNB1 mutation. These six cell lines were transfected with FZD7 cDNA together with a TOPflash reporter plasmid, resulting in a 1.5- to 24.3-fold increase of Tcf transcriptional activity. The mRNA expression levels of seven known Wnt target genes were also increased by 1.5- to 3.4-fold after transfection of FZD7 cDNA into HCT-116 cells. The six cell lines were then cotransfected with FZD7-siRNA and a TOPflash reporter plasmid, which reduced Tcf transcriptional activity to 20% to 80%. FZD7-siRNA was shown to significantly decrease cell viability and in vitro invasion activity after transfection into HCT-116 cells. Our present data demonstrated that FZD7 activates the canonical Wnt pathway in colon cancer cells despite the presence of APC or CTNNB1 mutation and that FZD7-siRNA may be used as a therapeutic reagent for CRCs.
Vacuum window optical power induced by temperature gradients (1999)

The Advanced Land Imager (ALI) of the EO-1 satellite has been calibrated under its normal operating conditions in a thermal vacuum chamber. The optical equipment for the calibrations had to be placed outside the chamber. The effects on the calibrating beam of the vacuum chamber window are described here. Since the instrument senses the reflected solar spectrum, the existing fused-silica window was found to have adequate spectral transmission properties. It also was measured to cause little wavefront error by itself. When the chamber is under vacuum, and the interior cold shroud is cooled to approximately 100 K, the window develops significant optical power. This is a result of radiational cooling of the window, coupled with change of its index of refraction with temperature. Such an effect seriously compromises the spatial calibrations of the instrument, particularly MTF measurements. This effect was overcome in two ways: A heating plate placed outside the window was used to alter the temperature distribution until its effect on the wavefront was negligible. The more practical solution was to measure the window power with an interferometer, and compensate for it by shifting the target reticle of the collimator.
<title>Wideband Real-Time Signal Processing Using Integrated Optics</title> (1980)

This paper presents a review of recent progress on planar guided-wave acoustooptics with application to wideband real-time signal processing and communications. Key parameters of an acoustooptic Bragg modulator/ deflector relevant to signal processing and communications are discussed first. A projection of the performance figures obtainable with these parameters using the existing technology is also made. Implementation of the acoustooptic time-integrating correlators using integrated optics techniques is then described.
Protein Adsorption in a Continuous Flow Microchannel Environment (2008)

Protein adsorption is a critical issue in microfluidic devices especially for those reactions depending on proteins like the polymerase chain reaction (PCR). Understanding protein absorption phenomena in different geometry microchannels and evaluating the efficiency of dynamic coating, which has been using as a method to prevent protein adsorption, are important tasks. Two different sets of microchannels were designed and fabricated on polymers. Bovine serum albumin (BSA) was used as a model protein for quantification of and monitoring the protein loss in different microchannel geometries. Up to 58% of the BSA was lost after flowing a 2030 mm long microchannel. The BSA adsorption rate changed along the microchannel. Smaller microchannels required a longer time to achieve protein saturation point. Dynamic coating was shown to be a time consuming and inefficient method to prevent protein adsorption in a continuous flow environment.
Simple Network Management Protocol Distributed Protocol Interface Version 2.0 (1994)

This memo defines an Experimental Protocol for the Internet community. This memo does not specify an Internet standard of any kind.
SYNTHESIS OF THE LINEAR B TYPE 2 TRISACCHARIDE Galα3Galβ4GlcNAcβOTMSEt, AND COUPLING OF THE CORRESPONDING 2-CARBOXYETHYL β-THIOGLYCOSIDE TO SEPHAROSE (2002)

The synthesis of the Linear B type 2 trisaccharide (Galα3Galβ4GlcNAcβOTMSEt) and the corresponding 2-carboxyethyl β-thioglycoside is described, as well as coupling of the latter to Sepharose.