gebhart/patrepeval huggingface dataset:
Optimization of Human Endothelial Cell Attachment to Vascular Graft Polymers (1991)

Endothelial cells (EC) covering the blood-contacting surface of a prosthetic material could potentially enhance the subsequent nonthrombogenicity of the surface. In order to create such a surface, the EC must become attached to the surface, spread and ultimately form a monolayer. In this study we examined several factors that influence these processes. On ePTFE surfaces, surface pretreatment with human serum for 30 minutes at a concentration of 1.4 gm percent protein resulted in significantly more attached EC when compared to other concentrations or when compared to fetal calf serum or human serum albumin. The rate of EC spreading was strongly influenced by temperature, with a maximum occurring at 37 degrees C. During real-time video microscopy, it was noted that the rate of EC attachment and spreading was primarily dependent on arrival of the EC to the surface rather than attachment and spreading. Thus as a method of increasing EC delivery, the concept of filtering EC onto the graft lumenal surface was tested by pressurizing the graft lumen to speed EC delivery to the surface. This technique produced a 2 to 5-fold increase in EC attachment when compared to gravity forced cell deposition. We conclude that an ePTFE graft can be rapidly endothelialized using these simple measures.
Modeling of the acoustic wave equation with transform methods (1981)

Abstract Numerical methods are described for the simulation of wave phenomena with application to the modeling of seismic data. Two separate topics are studied. The first deals with the solution of the acoustic wave equation. The second topic treats wave phenomena whose direction of propagation is restricted within + or -90 degrees from a given axis.In the numerical methods developed here, the wave field is advanced in time by using standard time differencing schemes. On the other hand, expressions including space derivative terms are computed by Fourier transform methods. This approach to computing derivatives minimizes truncation errors. Another benefit of transform methods becomes evident when attempting to restrict propagation to upward moving waves, e.g., to avoid multiple reflections. Constraints imposed on the direction of the wave propagation are accomplished most precisely in the wavenumber domain.The error analysis of the algorithms shows that truncation errors are due mainly to time discretization. Such errors can be limited by the choice of the time step. Perhaps the most significant error phenomenon is related to aliasing. This becomes noticeable when a narrow pulse traverses a region with strong velocity variations. It is shown that aliasing errors can be limited by the choice of the pulse width. The feasibility of these modeling methods is demonstrated on numerical examples.
Structural Response of Piping to Internal Gas Detonation (2006)

Detonation waves in gas-filled piping or tubing pose special challenges in analysis and prediction of structural response. The challenges arise due the nature of the detonation process and the role of fluid-structure interaction in determining the propagation and arrest of fractures. Over the past ten years, our laboratory has been engaged in studying this problem and developing methodologies for estimating structural response. A brief overview of detonation waves and some key issues relevant to structural waves is presented first. This is followed by a summary of our work on the elastic response of tubes and pipes to ideal detonation loading, highlighting the importance of detonation wave speed in determining flexural wave excitation and possibility of resonant response leading to large deformations. Some issues in measurement technique and validation testing are then presented. The importance of wave reflection from bends, valves and dead ends is discussed, as well as the differences between detonation, shock wave, and uniform internal pressure loading. Following this, we summarize our experimental findings on the fracture threshold of thin-walled tubes with pre-existing flaws. A particularly important issue for hazard analysis is the estimation of loads associated with flame acceleration and deflagration-to-detonation transition. We give some recent results on pressure and elastic strain measurements in the transition regime for a thick-wall piping, and some remarks about plastic deformation.
Fabrication of MEMS ZnO dome-shaped-diaphragm transducers for high frequency ultrasonic imaging (2005)

This paper describes the microfabrication technique for a dome-shaped-diaphragm transducer (DSDT) for 200 MHz cellular microstructure imaging. The DSDT uses a piezoelectric ZnO film to generate acoustic waves and is fabricated on a Si substrate. The DSDTs have been tested with a pulse echo method using a quartz target, and shown to produce an echo signal at 210 MHz with 20% bandwidth. The DSDT fabrication uses spherical balls to precisely shape wax molds, onto which parylene is deposited as a support layer for the DSDT. The ZnO film is sputter-deposited on the parylene dome diaphragm. E-solder silver epoxy is placed and cured on the back surface to function both as an acoustic backing and as a structural support. Quarter wavelength thick parylene is deposited on the front side of the wafer for acoustic matching. The fabrication technique for the DSDTs is meant for low-cost mass production of the devices for HF biomedical imaging. Moreover, the technique allows for a precise control of the radius and curvature of the DSD by adjusting the size of the front-to-backside thru holes using different radii of the spherical balls.
The molecular and biochemical characterization of mutant monoclonal antibodies with increased antigen binding (1991)

Mutant mAb with increased Ag binding were generated from a hybridoma cell line, 36-65, that secretes an IgG1,kappa anti-p-azophenylarsonate-(Ars) specific antibody. The mutant antibodies were identified using an Ars-specific ELISA and sib selection so that approximately 10(6) cells could be analyzed. The ELISA used as Ag a low ratio of Ars coupled to BSA and was set up so that only those antibodies that had higher binding than the parent would be detected. Seven mutant producing cell lines were isolated from five independent clones of 36-65. The mutant antibodies bind Ag 20 to more than 200-fold better than the parent and have wild type V region sequences. All have C region mutations that result in an increased avidity. At least five different genetic events are responsible for the C region mutations.
Reflectivity-induced variation in implant layer lithography (2008)

Scaling of designs to the 45nm and future nodes presents challenges for block level lithography. Shrinking distances between devices drive aggressive resist placement tolerances, challenging the ability to control critical dimension (CD). In particular, the potential variation in shallow trench isolation oxide may result in variation of resist profile and CD, thereby affecting edge placement accuracy. Potential sources of this include wafer-to-wafer or within-wafer STI trench depth variations, and STI CMP variations that may be induced by active area pattern density fluctuations. Some other potential sources of CD fluctuation include oxide sidewall variation, and implant level overlay or CD errors modulating the proximity to the oxide sidewall. Depending on the actual variation of isolation oxide and the exposure latitude of the resist, the CD variations simply from oxide variation may consume a large portion of the CD budget. Several examples are given of variations in resist profile and CD arising from these substrate effects. The CD uniformity of a test structure was shown to decrease dramatically with the addition of a BARC to the resist stack, most likely due to the suppression of substrate reflectivity variations. Simulations performed using Panoramic Technologies software demonstrated the potential sensitivity of the factors outlined above on CD and profile errors. A comparison of simulated vs. experimental results is made for a case of intentional overlay error, showing the failure mode of the resist profile as the mask edge passes from STI to the active area. The simulations using a full physical model provided with the simulation software predict a resist foot forming over the active area, which was confirmed experimentally.
Dynamic and stochastic models with freight distribution applications (1996)

Thanks to my family; my father Robert and my mother Johanna, and my sisters Maryke and Annaleen, for their encouragement, correspondence and visits, and for having me in their thoughts so often. It is their love and support through the years that made this achievement possible. ii
Vascular Smooth Muscle Cell Endovascular Therapy Stabilizes Already Developed Aneurysms in a Model of Aortic Injury Elicited by Inflammation and Proteolysis (2004)

VSMCs endovascular seeding restores the healing capabilities of proteolytically injured extracellular matrix in aneurysmal aortas, and stops expansion.
Inkjet Printing of Phase-Change Materials With Xaar1001 Printheads (2010)

Inkjet printing of phase-change or hot melt materials allows to produce etch masks by digital and additive processing, thus enabling highly efficient fabrication of printed circuit boards, solar cells etc. Previously the binary inkjet printing with drop volumes of 50 pL was demonstrated with Xaar126 end-shooter printheads. The present work describes new results on printing with Xaar1001 printheads. These printheads offer both, grayscale printing with a subdrop volume of 6 pL and excellent reliability by way of their unique ‘true throughflow’ capability, respectively.While the advantage of grayscale printing for high-resolution patterning is obvious and straight forward, the complexity lies in the recirculating ink systems that provide the constant ink flow through the printhead channels. A narrow temperature range has to be established throughout the total ink path, which avoids hot spots that would damage the inks as well as cold spots that counteract the ink flow rate. Print results with Sunjet Crystal HEP9520, operating between 80 °C and 90 °C, clearly show good droplet formation for 7 distinct gray levels spanning a range from 5 to 37 pL with increments of 5.3 pL at a frequency of 5 kHz.
Orthogonal Self-Assembly of Low Molecular Weight Hydrogelators and Surfactants (2003)

The concurrent self-assembly of new 1,3,5-trisamide-cyclohexane-based low molecular weight hydrogelators and various surfactants in water leads to the formation of self-assembled fibrillar networks with encapsulated micelles. This prototype system presents an example of orthogonal self-assembly, that is, the independent formation of two different supramolecular structures, each with their own characteristics that coexist within a single system.
On the Evaluation of VM Provisioning Time in Cloud Platforms for Mission-Critical Infrastructures (2014)

Cloud Computing has risen a great interest over the last years as it represents an enabling technology for flexible and ubiquitous access over the network to a set of shared computing resources. This work comes from an industrial experience aiming at exploiting the cloud potential for virtualizing complex infrastructures such as an entire Air Traffic Center (ATC), a clear example of complex SoS (System of Systems). The use of virtualization is convenient because industry can leverage in-house test-beds to perform distributed testing campaigns in pre-operational phases or to design new automatic fail-over mechanisms for fully distributed systems. In order to realize such mitigation and recovery techniques in the ATC field, indeed, a cloud platform is required to guarantee a low VM provisioning time with the objective of minimizing the service disruption. In this perspective, after having introduced the principal concepts and factors of the provisioning time, we propose a deep analysis and comparison for two different Infrastructure-as-a-Service (IaaS) platforms, namely Open Stack and Open Nebula (using KVM as hypervisor), that were selected through a preliminary scouting phase.
Turbulent Flow Measurements by Laser-Doppler Anemometry in Motored Piston-Cylinder Assemblies (1979)

Laser-Doppler anemometry has been used to quantify the mean velocity and turbulence characteristics of the isothermal, incompressible flow within a piston-cylinder arrangement motored without compression at 200 rpm and with idealized inlet geometries corresponding to a pipe and to an annular port located in the centre of the cylinder head. The results indicate that the pipe entry gives rise to a strong vortex near the piston as the indrawn air is deflected radially along the piston face and cylinder wall; this, in turn, gives rise to a weaker, counter-rotating vortex near the cylinder head which grows appreciably as the piston approaches bottom-dead-centre. With the annular-port entry, the inlet jet is angled and results in a flow pattern with a large vortex occupying nearly all of the flow space with much smaller vortices at the corners between the wall and the piston and cylinder heads. The effect of a piston bowl was also investigated for the port entry and is shown to be small.
Studies Using an in Vitro Model Show Evidence of Involvement of Epithelial-Mesenchymal Transition of Human Endometrial Epithelial Cells in Human Embryo Implantation (2011)

Human embryo implantation is a critical multistep process consisting of embryo apposition/adhesion, followed by penetration and invasion. Through embryo penetration, the endometrial epithelial cell barrier is disrupted and remodeled by an unknown mechanism. We have previously developed an in vitro model for human embryo implantation employing the human choriocarcinoma cell line JAR and the human endometrial adenocarcinoma cell line Ishikawa. Using this model we have shown that stimulation with ovarian steroid hormones (17β-estradiol and progesterone, E2P4) and suberoylanilide hydroxamic acid (SAHA), a histone deacetylase inhibitor, enhances the attachment and adhesion of JAR spheroids to Ishikawa. In the present study we showed that the attachment and adhesion of JAR spheroids and treatment with E2P4 or SAHA individually induce the epithelial-mesenchymal transition (EMT) in Ishikawa cells. This was evident by up-regulation of N-cadherin and vimentin, a mesenchymal cell marker, and concomitant down-regulation of E-cadherin in Ishikawa cells. Stimulation with E2P4 or SAHA accelerated Ishikawa cell motility, increased JAR spheroid outgrowth, and enhanced the unique redistribution of N-cadherin, which was most prominent in proximity to the adhered spheroids. Moreover, an N-cadherin functional blocking antibody attenuated all events but not JAR spheroid adhesion. These results collectively provide evidence suggesting that E2P4- and implanting embryo-induced EMT of endometrial epithelial cells may play a pivotal role in the subsequent processes of human embryo implantation with functional control of N-cadherin.
Layered Paving of Vesicular Nanoparticles Formed with Cerasome as a Bioinspired Organic−Inorganic Hybrid (2002)

A three-dimensional packed vesicular assembly was successfully prepared by using an organic-inorganic hybrid, the Cerasome. This assembly was achieved by using an alternate layer-by-layer adsorption technique with the anionic and the cationic Cerasome derived from corresponding organoalkoxysilane amphiphiles. Adsorption quantities of each Cerasome layer were evaluated by employing a quartz crystal microbalance. The surface structure of the Cerasome paving on a substrate obtained in this way was observed by atomic force microscopy. The Cerasome particles closely packed like a stone pavement were clearly observed in both layers. In addition, the difference in the particle size for each layer indicates the cationic and anionic Cerasomes undoubtedly formed the layer-by-layer assembly. The layered paving of the vesicular nanoparticles was seen in every layer at least up to ten adsorption steps.
Role of interceptive orthodontics in early mixed dentition. (2004)

Early orthodontic interventions are often initiated in the developing dentition to promote favourable developmental changes and remove or suppress those that are unfavourable. Early interceptive orthodontics can eliminate or reduce the severity of a developing malocclusion, the complexity of orthodontic treatment, overall treatment time and cost. It also improves self-esteem in the subjects and parental satisfaction. Early detection and appropriate referral of cases requiring interceptive orthodontics are important. However, lack of awareness among school children, parents and primary-care personnel (dental nurses and dental officers) may result in patients not being referred for timely interceptive intervention. This article presents a general view of the scope of interceptive orthodontics that can be carried out in early mixed dentition, i.e. when the permanent incisors and molars are erupting into the oral cavity.
Possible His to Asp phosphorelay signaling in an <i>Arabidopsis</i> two‐component system (2000)

We have so far cloned a cDNA encoding a hybrid-type histidine kinase (ATHK1), three cDNAs encoding phosphorelay intermediates (ATHP1-3), and four cDNAs encoding response regulators (ATRR1-4) from Arabidopsis thaliana. To determine which molecules constitute a His to Asp phosphorelay pathway, we examined protein-protein interactions between them using a pairwise yeast two-hybrid analysis, as an initial step. We detected a specific interaction between ATHK1 and ATHP1. We further examined protein-protein interactions between ATHP1-3 and other histidine kinases. We detected interactions between ETR1 and all ATHPs, and between CKI1 and ATHP1 or ATHP2. Interestingly, ERS1 could not interact with any ATHPs. We also examined protein-protein interactions between ATHP1-3 and ATRR1-4. The results indicated that ATHP2 could interact with ATRR4, and that ATHP3 could interact with ATRR1 or ATRR4. However, ATHP1 could not interact with any ATRRs. On the basis of these results, we discuss the possible phosphorelay networks in an Arabidopsis two-component system.
Screening for Breast Cancer: U.S. Preventive Services Task Force Recommendation Statement (2016)

The USPSTF recommends biennial screening mammography for women aged 50 to 74 years. (B recommendation) The decision to start screening mammography in women prior to age 50 years should be an individual one. Women who place a higher value on the potential benefit than the potential harms may choose to begin biennial screening between the ages of 40 and 49 years. (C recommendation) The USPSTF concludes that the current evidence is insufficient to assess the balance of benefits and harms of screening mammography in women aged 75 years or older. (I statement) The USPSTF concludes that the current evidence is insufficient to assess the benefits and harms of digital breast tomosynthesis (DBT) as a primary screening method for breast cancer. (I statement) The USPSTF concludes that the current evidence is insufficient to assess the balance of benefits and harms of adjunctive screening for breast cancer using breast ultrasonography, magnetic resonance imaging (MRI), DBT, or other methods in women identified to have dense breasts on an otherwise negative screening mammogram. (I statement).
Insulin inhibits vascular smooth muscle contraction at a site distal to intracellular Ca<sup>2+</sup>concentration (1998)

Several hypertensive states are associated with resistance to insulin-induced glucose disposal and insulin-induced vasodilation. Insulin can inhibit vascular smooth muscle (VSM) contraction at the level of the VSM cell, and resistance to insulin's inhibition of VSM cell contraction may be of pathophysiological importance. To understand the VSM cellular mechanisms by which insulin resistance leads to increased VSM contraction, we sought to determine how insulin inhibits contraction of normal VSM. It has been shown that insulin lowers the contractile agonist-stimulated intracellular Ca2+ (Ca2+i) transient in VSM cells. In this study, our goal was to see whether insulin inhibits VSM cell contraction at steps distal to Ca2+i and, if so, to determine whether the mechanism is dependent on nitric oxide synthase (NOS) and cGMP. Primary cultured VSM cells from canine femoral artery were bathed in a physiological concentration of extracellular Ca2+ and permeabilized to Ca2+ with a Ca2+ ionophore, either ionomycin or A-23187. The resultant increase in Ca2+i contracted individual cells, as measured by photomicroscopy. Preincubating cells with 1 nM insulin for 30 min did not affect basal Ca2+i or the ionomycin-induced increase in Ca2+i, as determined by fura 2 fluorescence measurements, but it did inhibit ionomycin- and A-23187-induced contractions by 47 and 51%, respectively (both P < 0.05). In the presence of 1.0 microM ionized Ca2+, ionomycin-induced contractions were inhibited by insulin in a dose-dependent manner. In the presence of ionomycin, insulin increased cGMP production by 43% (P < 0.05). 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (10 microM), a selective inhibitor of guanylate cyclase that blocked cGMP production in these cells, completely blocked the inhibition by insulin of ionomycin-induced contraction. It was found that the cells expressed the inducible isoform of NOS. NG-monomethyl-L-arginine or NG-nitro-L-arginine methyl ester (0.1 mM), inhibitors of NOS, did not affect ionomycin-induced contraction but prevented insulin from inhibiting contraction. We conclude that insulin stimulates cGMP production and inhibits VSM contraction in the presence of elevated Ca2+i. This inhibition by insulin of VSM contraction at sites where Ca2+i could not be rate limiting is dependent on NOS and cGMP.
Incremental tools for the design and verification of VLSI circuits (1993)

Although shrinking geometries and larger VLSI chips have produced faster systems, they have also imposed greater demands on the speed of the design tools. These demands are exacerbated by the iterative nature of the design process whereby circuits are repeatedly modified and verified.
The VLSI design tools described in this thesis reduce design time by operating incrementally. An incremental tool takes advantage of the fact that most design changes affect only part of the circuit; thus, many of the computations performed while verifying a modified design are identical to those performed during previous verification runs. By reusing previous results, incremental tools can confine their work to the sections of the design affected by the changes, thereby allowing modifications to be verified in a time proportional to the size of the changes rather than the size of the design.
The incremental system described in this thesis consists of two components: simulator and circuit extractor. The simulator, Irsim, is a logic plus timing simulator that employs a switched-resistor MOSFET model that accurately simulates circuits at the transistor level. Irsim allows designers to modify the circuit being simulated and then incrementally update the waveforms of the sections of the circuit whose behavior is altered by the changes. To accomplish this, Irsim maintains a history of circuit activity during simulation and only resimulates the sections of the circuit that deviate from their history.
The circuit extractor is embedded within a hierarchical layout editor; it operates by identifying circuit changes from the layout modifications, and producing the circuit-level transformations needed to update the circuit's network. To accomplish this, the extractor keeps track of the modified layout areas and then extracts only the circuits contained within the modified areas from the layouts before and after the modifications; it then compares the graphs of the extracted subcircuits and reports the differences as network transformations.
Applicability of the system is demonstrated by stepping through the final phases of a large VLSI design. The results show that the incremental tools reduced design cycle runtime by an average of two orders of magnitude.