gebhart/patrepeval huggingface dataset:
Use of a plant enzyme preparation (Coronase) instead of hyaluronidase for cumulus cell removal before intracytoplasmic sperm injection (1998)

The aim of this study was to compare the efficiency of a plant enzyme preparation (Coronase) with animal extracted hyaluronidase to remove cumulus cells before intracytoplasmic sperm injection (ICSI). The first part of the study was performed on mouse oocytes and embryos. Coronase displayed a similar efficiency to that of hyaluronidase for removing cumulus cells and the same percentage of activated oocytes was obtained with both techniques. However, prolonged incubation in Coronase, 120 min, led to a degeneration of oocytes. Incubation of 2-cell mouse embryos for 10 min with Coronase did not affect their subsequent in-vitro development to blastocyst. Coronase was then compared to hyaluronidase in the treatment of human oocytes prior to ICSI. The time required for total denudation was slightly longer using Coronase (98 s +/- 25 s versus 84 s +/- 24 s respectively for Coronase and hyaluronidase; P < 0.01). However, the two pronuclear (2PN) fertilization rate (70/103 versus 63/107 respectively for Coronase and hyaluronidase, not significant) and the percentage of embryos with a good morphology (39/74 versus 32/67 respectively for Coronase and hyaluronidase, not significant) were identical with both treatments. In conclusion, Coronase displays an efficiency close to that of hyaluronidase, without any adverse effect on oocytes, and may be preferable for human use.
Stray signal requirements for compact range reflectors based on RCS measurement errors (1991)

The authors present a performance criterion for compact range reflectors such that their edge diffracted stray signal levels meet a reasonable radar cross section (RCS) measurement error requirement. It is shown by example that one of the significant error sources is the diffracted fields emanating from the edges or junctions of the reflector. This measurement error is demonstrated by placing a diagonal square flat plate in the target zone and rotating it to appropriate angles. These angles are determined by bisecting the plane wave and stray signal directions. This results in a peak bistatic measurement of the edge diffracted stray signal. It is proposed that the diagonal flat plate be used to evaluate new reflector designs as well as existing systems. A reasonable stray signal performance level has been developed so that new reflector systems can be characterized in terms of an RCS measurement error requirement.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
A Block Interconnection Algorithm for Hierarchical Layout System (1987)

A block interconnection algorithm for a general cell VLSI is described, which consists of a number of procedures such as a global router with signal delays taken into account, a router for power and ground, a block positioning scheme to minimize the chip size, a channel construction scheme with the use of L-shaped channels, and a grid-free channel router. The algorithm has been employed in a layout design system SMILE for general cell VLSI's for more than one year. Some of the experimental results are also shown.
InP HBT IC Technology for Terahertz Frequencies: Fundamental Oscillators Up to 0.57 THz (2011)

We report on the development of a 0.25-μm InP HBT IC technology for lower end of the THz frequency band (0.3-3 THz). Transistors demonstrate an extrapolated f <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">max</sub> of >;800 GHz while maintaining a common-emitter breakdown voltage (BVCEO) >;4 V. The transistors have been integrated in a full IC process that includes three-levels of interconnects, and backside processing. The technology has been utilized for key circuit building blocks (amplifiers, oscillators, frequency dividers, PLL, etc), all operating at ≥300 GHz. Next, we report a series of fundamental oscillators operating up to 0.57 THz fabricated in a 0.25-μm InP HBT technology. Oscillator designs are based on a differential series-tuned topology followed by a common-base buffer, in a fixed-frequency or varactor-tuned scheme. For ≥400 GHz designs, a subharmonic down-conversion mixer is integrated to facilitate spectrum measurement. At optimum bias, the measured output power was -6.2, -5.6, and -19.2 dBm, for 310.2-, 412.9-, and 573.1-GHz designs, respectively, with P <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">DC</sub> ≤ 115 mW. Varactor-tuned designs demonstrated 10.6-12.3 GHz of tuning bandwidth up to 300 GHz.
Transforming growth factor <i>β</i> (TGF<i>β</i>) causes a persistent increase in steady-state amounts of type I and type III collagen and fibronectin mRNAs in normal human dermal fibroblasts (1987)

It has been previously shown that transforming growth factor beta (TGF beta) is capable of stimulating fibroblast collagen and fibronectin biosynthesis. The purpose of this study was to examine the mechanisms involved in TGF beta stimulation of fibroblast biosynthetic activity. Our results indicate that TGF beta causes a marked enhancement of the production of types I and III collagens and fibronectin by cultured normal human dermal fibroblasts. The rate of collagen production by fibroblasts exposed to TGF beta was 2-3-fold greater than that of control cells. These effects were associated with a 2-3-fold increase in the steady-state amounts of types I and III collagen mRNAs and a 5-8-fold increase in the amounts of fibronectin mRNAs as determined by dot-blot hybridization with specific cloned cDNA probes. In addition, the increased production of collagen and fibronectin and the increased amounts of their corresponding mRNAs remained elevated for at least 72 h after removal of TGF beta. These findings suggest that TGF beta may play a major role in the normal regulation of extracellular matrix production in vivo and may contribute to the development of pathological states of fibrosis.
The Influence of Avatars on Online Consumer Shopping Behavior (2006)

An impediment to Web-based retail sales is the impersonal nature of Web-based shopping. A solution to this problem is to use an avatar to deliver product information. An avatar is a graphic representation that can be animated by means of computer technology. Study 1 shows that using an avatar sales agent leads to more satisfaction with the retailer, a more positive attitude toward the product, and a greater purchase intention. Study 2 shows that an attractive avatar is a more effective sales agent at moderate levels of product involvement, but an expert avatar is a more effective sales agent at high levels of product involvement.
Boron-Bridged Group-4ansa-Metallocene Complexes (2001)

Boron-bridged group-4 ansa-metallocene complexes are newcomers to the field of metallocene-mediated olefin polymerization catalysis. Because of the Lewis acidity of the boron bridge, it is more reactive than the more common carbon and silicon bridges. It is also more vulnerable to cleavage from the ligand framework by nucleophilic agents. A primary goal of our work is to involve the boron bridge more actively in the reaction chemistry of the metallocene while keeping the bridge intact.
DEPLETION OF GLUTATHIONE S -TRANSFERASE P1 INDUCES APOPTOSIS IN HUMAN LUNG FIBROBLASTS (2003)

Glutathione S-transferase P1 (GSTP1) is one of the xenobiotic-metabolizing and antioxidant enzymes, identified in the peripheral lungs. Recently, the authors reported the association between GSTP1 gene polymorphism and susceptibility to chronic obstructive pulmonary disease (COPD), and protective effect of GSTP1 against cigarette smoke in human lung fibroblasts in vitro. In this study, the authors investigated that depletion of GSTP1 by itself could induce cell death, including apoptosis, in human lung fibroblast-derived HFL-1 cells. The level of apoptosis and necrosis was increased significantly with GSTP1 antisense vector transfection. It was also observed that the transfection efficiency and the expression level of the vector were weaker in the transfectant of the antisense vector than in those of the sense and control vectors, which is also thought to indicate that inhibition of GSTP1 expression by the antisense vector alone affects cellular viability. However, there was no difference among these transfectants neither on glutathione (GSH) level nor on c-Jun NH2-terminal kinase (JNK) activation. Therefore, the authors report here that underexpression of GSTP1 appeared to induce apoptosis on lung fibroblasts, which suggests that GSTP1 may have protective effects against apoptosis in the airway cells, though the mechanism of this apoptotic pathway is still to be elucidated.
107. The stereochemistry of hydroxyproline (1945)

A. Neuberger, J. Chem. Soc., 1945, 429 DOI: 10.1039/JR9450000429
Immunocytochemical localization of cathepsin D in rat ventral prostate: Evidence for castration‐induced expression of cathepsin D in basal cells (1991)

Abstract Cathepsin D (EC3.4.23.5) is an aspartyl endopeptidase involved in lysosomal proteolysis. Its functional role is uncertain. This study was undertaken to determine the cellular and subcellular distribution of cathepsin D in the normal rat ventral prostate and its possible role in the castration‐induced atrophy of the gland. Cathepsin D was localized immunohistochemically to perinuclear lysosomes in secretory cells, in capillary endothelial cells, and, occasionally, in stromal cells of the untreated animal. Castration resulted in an increased number of cathepsin D‐positive cells in the stroma within 24 hr. By 48 hr after castration autophagolysosomes formed in secretory cells and apoptotic bodies appeared in the epithelium. Although apoptotic bodies generally contained immunoreactive cathepsin D, a subpopulation of larger apoptotic bodies, which commonly rested on the basement membrane and contained multiple inclusions, were more variable in cathepsin D expression. The induction of cathepsin D in dendritic cells basally oriented in the epithelium was noted at 4 days of castration. These cells had a phagocytic phenotype, were distributed periodically along the basement membrane, and were not found in ductal epithelia. Treatment with actinomycin D or hydrocortisone to reduce the rate of regression of the ventral prostate blocked the appearance of these cathepsin D‐positive, basally oriented epithelial cells. Our data indicate that this cathepsin D‐positive, phagocytic cell differentiates from a cell resident in the prostatic epithelium. We suggest that it differentiates from basal cells in the secretory tubuloalveolar portion of the gland and that it is involved in the destruction of regressed secretory cells.
DDA: Cross-Session Throughput Prediction with Applications to Video Bitrate Selection (2015)

User experience of video streaming could be greatly improved by selecting a high-yet-sustainable initial video bitrate, and it is therefore critical to accurately predict throughput before a video session starts. Inspired by previous studies that show similarity among throughput of similar sessions (e.g., those sharing same bottleneck link), we argue for a cross-session prediction approach, where throughput measured on other sessions is used to predict the throughput of a new session. In this paper, we study the challenges of cross-session throughput prediction, develop an accurate throughput predictor called DDA, and evaluate the performance of the predictor with real-world datasets. We show that DDA can predict throughput more accurately than simple predictors and conventional machine learning algorithms; e.g., DDA's 80%ile prediction error of DDA is > 50% lower than other algorithms. We also show that this improved accuracy enables video players to select a higher sustainable initial bitrate; e.g., compared to initial bitrate without prediction, DDA leads to 4x higher average bitrate.
Distributed algorithms for guiding navigation across a sensor network (2003)

We develop distributed algorithms for self-organizing sensor networks that respond to directing a target through a region. The sensor network models the danger levels sensed across its area and has the ability to adapt to changes. It represents the dangerous areas as obstacles. A protocol that combines the artificial potential field of the sensors with the goal location for the moving object guides the object incrementally across the network to the goal, while maintaining the safest distance to the danger areas. We give the analysis to the protocol and report on hardware experiments using a physical sensor network consisting of Mote sensors.
Tryptic digestion of the alpha subunit of human choriogonadotropin. (1986)

In order to further characterize chemical, physicochemical, and immunochemical properties, as well as structure-function relationships, of the common alpha subunit of human glycoprotein hormones, a tryptic core was prepared from the alpha subunit of human choriogonadotropin. The core was purified in greater than 80% yield using gel permeation and anion-exchange chromatography, and, following reduction and S-carboxymethylation, the constituent peptides were purified by gel permeation and high performance liquid chromatography. The disulfide-bridged peptides comprising the alpha core were identified as residues 1-35 and residues 52-91 by amino acid composition and amino acid carboxyl sequence analyses of the reduced, S-carboxymethylated peptides. The alpha tryptic core contained both N-asparagine carbohydrate moieties, but was devoid of residues 36-51 and the carboxyl-terminal serine at position 92. The small peptides cleaved from residues 36-51, a known potential O-glycosylation region of the alpha subunit, were purified and identified. The tryptic core retained full immunopotency relative to the intact subunit in the binding to polyclonal and monoclonal antibodies directed against the alpha subunit. The region consisting of residues 36-51 is not part of the epitope recognized by these antibodies. With antisera generated to the reduced, S-carboxymethylated subunit, peptide 1-35, but not 52-91, was immunoreactive. This finding is consistent with the known dominant antigenicity of the amino-terminal region in the reduced, S-carboxymethylated molecule. The core exhibited no appreciable interaction with the complementary beta subunit, and, not surprisingly, was unable to compete with intact hormone binding in a radioreceptor assay using rat testicular homogenates. Circular dichroic spectroscopy was used to probe gross features of tertiary structure (240-300 nm) and secondary structure (190-240 nm). The tryptic core and each of the two constituent peptides exhibited spectra above 240 nm that resembled that of the reduced, S-carboxymethylated subunit more than that of the native material, thus suggesting a significant loss of tertiary structure in the core and isolated peptides. This finding is unexpected in consideration of the full retention of immunopotency by the alpha core although consistent with failure of the core to combine with intact complementary beta subunit. The intact subunit as well as the isolated constituent peptides exhibit little if any helicity in aqueous solution. Interestingly, the reduced, S-carboxymethylated chain and peptide 52-91 displayed helicity in 80% trifluoroethanol, a helicogenic solvent.
An improved, low-cost, hydroponic system for growing Arabidopsis and other plant species under aseptic conditions (2014)

The efficiency and functionality of our proposed hydroponic system is demonstrated in nutrient deficiency and pathogenesis experiments. Hydroponically grown Arabidopsis seedlings under long-time inorganic phosphate (Pi) deficiency showed typical changes in root architecture and high expression of marker genes involved in signaling and Pi recycling. Genome-wide transcriptional analysis of gene expression of Arabidopsis roots depleted of Pi by short time periods indicates that genes related to general stress are up-regulated before those specific to Pi signaling and metabolism. Our hydroponic system also proved useful for conducting pathogenesis essays, revealing early transcriptional activation of pathogenesis-related genes.
연구논문 : 소비자 참여에 대한 감정적 반응의 차원과 영향에 관한 연구: 정보교환 참여행동을 중심으로 (2012)

서비스 산업에서 소비자의 역할이 증가함에 따라 소비자 참여의 중요성이 부각되고 있다. 이처럼 서비스의 생산과 전달과정에서 소비자 참여에 관심을 가지는 것은 소비자 참여가 소비자들이 지각하는 서비스 품질뿐만 아니라 소비자 만족과 같은 서비스 결과 향상에 중요한 영향을 미치기 때문이다. 그동안 선행연구들에서는 소비자 참여가 서비스 결과에 미치는 영향을 고려함에 있어서 감정적인 측면을 간과하고 있었다. 최근 소비자 참여와 관련한 연구들이 증가하였으나 소비자 참여와 서비스성과사이의 관계에서 감정적인 부분을 함께 고려한 연구는 없었다. 감정은 서비스 품질의 평가와 만족에 영향을 줄 수 있는 중요한 변수이므로 이에 대한 검토는 긴요한 과제이다. 이러한 문제의식을 토대로 본 연구는 소비자 참여와 서비스성과의 관계에서 소비자들이 느끼는 다양한 감정차원이 어떠한 영향을 미치는지를 검토해 보았다. 실증분석 결과 서비스 접점에서 적극적인 참여를 하는 동안에 느끼는 감정은 정서적 공감, 예방적 감정과 향상적 감정의 3가지 유형으로 구분되었다. 먼저, 소비자 참여는 3가지 유형의 감정차원에 모두 긍정적인 영향을 미치는 것으로 나타났으며, 서비스 품질에도 긍정적인 영향을 미치는 것으로 나타났다. 그리고 3가지 유형의 감정차원은 서비스 품질에는 모두 유의한 영향을 미치는 것으로 나타났으나 소비자 만족에는 감정 차원별로 영향이 다른 것으로 나타났다.
Modification of Silicone Elastomer Surfaces with Zwitterionic Polymers: Short-Term Fouling Resistance and Triggered Biofouling Release (2015)

We present a method for dual-mode-management of biofouling by modifying surface of silicone elastomers with zwitterionic polymeric grafts. Poly(sulfobetaine methacrylate) was grafted from poly(vinylmethylsiloxane) elastomer substrates using thiol-ene click chemistry and surface-initiated, controlled radical polymerization. These surfaces exhibited both fouling resistance and triggered fouling-release functionality. The zwitterionic polymers exhibited fouling resistance over short-term (∼hours) exposure to bacteria and barnacle cyprids. The biofilms that eventually accumulated over prolonged-exposure (∼days) were easily detached by applying mechanical strain to the elastomer substrate. Such dual-functional surfaces may be useful in developing environmentally and biologically friendly coatings for biofouling management on marine, industrial, and biomedical equipment because they can obviate the use of toxic compounds.
Privacy-Preserving Decentralized Key-Policy Attribute-Based Encryption (2012)

Decentralized attribute-based encryption (ABE) is a variant of a multiauthority ABE scheme where each authority can issue secret keys to the user independently without any cooperation and a central authority. This is in contrast to the previous constructions, where multiple authorities must be online and setup the system interactively, which is impractical. Hence, it is clear that a decentralized ABE scheme eliminates the heavy communication cost and the need for collaborative computation in the setup stage. Furthermore, every authority can join or leave the system freely without the necessity of reinitializing the system. In contemporary multiauthority ABE schemes, a user's secret keys from different authorities must be tied to his global identifier (GID) to resist the collusion attack. However, this will compromise the user's privacy. Multiple authorities can collaborate to trace the user by his GID, collect his attributes, then impersonate him. Therefore, constructing a decentralized ABE scheme with privacy-preserving remains a challenging research problem. In this paper, we propose a privacy-preserving decentralized key-policy ABE scheme where each authority can issue secret keys to a user independently without knowing anything about his GID. Therefore, even if multiple authorities are corrupted, they cannot collect the user's attributes by tracing his GID. Notably, our scheme only requires standard complexity assumptions (e.g., decisional bilinear Diffie-Hellman) and does not require any cooperation between the multiple authorities, in contrast to the previous comparable scheme that requires nonstandard complexity assumptions (e.g., q-decisional Diffie-Hellman inversion) and interactions among multiple authorities. To the best of our knowledge, it is the first decentralized ABE scheme with privacy-preserving based on standard complexity assumptions.
Menu Auctions, Resource Allocation, and Economic Influence (1986)

In many examples of competitive bidding (e.g., government construction contracting) the relevant object is either partially divisible or ill-defined, in contrast to much of the recent theoretical work on auctions. In this paper we consider a more general class of auctions, in which bidders name a "menu" of offers for various possible actions (allocations) available to the auctioneer. We focus upon "first-price" menu auctions under the assumption of complete information, and show that, for an attractive refinement of the set of Nash Equilibria, an efficient action always results. Our model also has application to situations of economic influence, in which interested parties independently attempt to influence a decision-maker's action.
Health hazards and nitrous oxide: a time for reappraisal. (1992)

Recent adoption by the American Conference of Governmental Industrial Hygienists of a Threshold Limit Value of 50 ppm for an 8-hour average exposure to nitrous oxide (N2O) increases the likelihood for its regulation by state and federal occupational health agencies. This review outlines current information on the health risks of N2O inhalation to provide a basis from which safe and reasonably attainable exposure limits can be proposed. Although N2O was for many years believed to have no toxicity other than that associated with its anesthetic action, bone marrow depression in patients administered N2O for extended periods of time and neurological abnormalities in health care workers who inhaled N2O recreationally have disproved this notion. Retrospective surveys of dental and medical personnel have also linked occupational exposure to N2O with a number of health problems and reproductive derangements. Nitrous oxide reacts with the reduced form of vitamin B12, thereby inhibiting the action of methionine synthase, an enzyme that indirectly supports methylation reactions and nucleic acid synthesis. Many, if not all, of the nonanesthetic-related adverse effects of N2O may be ascribed to this action. Animal and human studies indicate that the toxic effects of N2O are concentration- and time-dependent. It is suggested that a time-weighted average of 100 ppm for an 8-hour workday and/or a time-weighted average of 400 ppm per anesthetic administration would provide adequate protection of dental personnel and be achievable with existing pollution control methods.
Gold Nanohole Array Substrates as Immunobiosensors (2008)

A gold nanohole array is functionalized with a cortisol thiol derivative, and binding to a monoclonal antibody is conveniently detected using the sensitive shift in the 1060 nm transmission peak of the array. Detection is also enhanced 3-fold by the application of a secondary antibody-gold nanoparticle conjugate. This regenerable response represents a more sensitive shift than that obtained previously for higher affinity binding and opens the way to application of nanohole arrays in immunobiosensing of important biomolecules.