gebhart/patrepeval huggingface dataset:
T-DNA organization in tumor cultures and transgenic plants of the monocotyledon <i>Asparagus officinalis</i> (1987)

Asparagus officinalis was the first monocotyledonous plant from which hormone-independent and opine-producing crown gall tissue could be isolated. We confirm by DNA hybridization that tumor lines obtained after infection of this plant by Agrobacterium strains harboring wild-type nopaline and octopine tumor-inducing (Ti) plasmids are stably transformed and contain transferred DNA (T-DNA) segments identical to the T-DNA found in dicotyledonous plants. We have also infected Asparagus with a nononcogenic T-DNA vector that carries a chimeric aminoglycoside phosphotransferase [NOS-APH(3')II] gene and selected transformed tissues on kanamycin-containing medium. The transformed status of these tissues was then confirmed by DNA hybridization. From these calli we regenerated kanamycin-resistant shoots that were subsequently rooted. Thus we report the isolation of transgenic monocotyledonous plants engineered via the Agrobacterium vector system.
Adaptive dynamic memory allocators by estimating application workloads (2012)

Modern applications are becoming more complex and dynamic and try to efficiently utilize the amount of available resources on the computing platforms. Efficient memory utilization is a key challenge for application developers, especially since memory is a scarce resource and often becomes systems bottleneck. Thus, the developers can resort to dynamic memory management, i.e., dynamic memory allocation and de-allocation, to efficiently utilize the memory resources. A high-performance adaptive memory allocator is presented in this paper. A memory allocator helps applications to manage more efficiently the memory space that operating systems bestow to them. In our approach, we tune the memory allocator at runtime by predicting the amount of memory to be requested. Experimental results obtained using applications from the PARSEC benchmark suite and dmmlib, a memory allocator framework written in C. Results show that adaptive memory allocators can improve the fragmentation problems leading to a more efficient memory usage.
Distill Knowledge from NRSfM for Weakly Supervised 3D Pose Learning (2019)

We propose to learn a 3D pose estimator by distilling knowledge from Non-Rigid Structure from Motion (NRSfM). Our method uses solely 2D landmark annotations. No 3D data, multi-view/temporal footage, or object specific prior is required. This alleviates the data bottleneck, which is one of the major concern for supervised methods. The challenge for using NRSfM as teacher is that they often make poor depth reconstruction when the 2D projections have strong ambiguity. Directly using those wrong depth as hard target would negatively impact the student. Instead, we propose a novel loss that ties depth prediction to the cost function used in NRSfM. This gives the student pose estimator freedom to reduce depth error by associating with image features. Validated on H3.6M dataset, our learned 3D pose estimation network achieves more accurate reconstruction compared to NRSfM methods. It also outperforms other weakly supervised methods, in spite of using significantly less supervision.
N- and O-Glycosylation Analysis of Etanercept Using Liquid Chromatography and Quadrupole Time-of-Flight Mass Spectrometry Equipped with Electron-Transfer Dissociation Functionality (2013)

Etanercept is a highly glycosylated therapeutic Fc-fusion protein that contains multiple N- and O-glycosylation sites. An in-depth characterization of the glycosylation of etanercept was carried out using liquid chromatography/mass spectrometry (LC/MS) methods in a systematic approach in which we analyzed the N- and O-linked glycans and located the occupied O-glycosylation sites. Etanercept was first treated with peptide N-glycosidase F to release the N-glycans. The N-glycan pool was labeled with a 2-aminobenzamide (2-AB) fluorescence tag and separated using ultraperformance liquid chromatography-hydrophilic interaction liquid chromatography (UPLC-HILIC). Preliminary structures were assigned using Glycobase. These assignments, which included monosaccharide sequence and linkage information, were confirmed by exoglycosidase array digestions of aliquots of the N-glycan pool. The removal of the N-glycans from etanercept facilitated the selective characterization of O-glycopeptides and enabled the O-glycans to be identified. These were predominantly of the core 1 subtype (HexHexNAc O-structure) attached to Ser/Thr residues. α2→3,6,8,9 sialidase was used to remove the sialic acid residues on the O-glycans allowing the use of an automated LC/MS(E) protocol to identify the O-glycopeptides. Electron-transfer dissociation (ETD) was then used to pinpoint the 12 occupied O-glycosylation sites. The determination of N- and O-glycans and O-glycosylation sites in etanercept provides a basis for future studies addressing the biological importance of specific protein glycosylations in the production of safe and efficacious biotherapeutics.
Finding topic-centric identified experts based on full text analysis (2007)

Abstract. This paper shows a method for finding topic-centric experts from open access metadata and full text documents. Topic-centric information including experts is served on OntoFrame, which is a Semantic Web-based academic research information service supporting R&amp;D activities. URI schemebased OntoFrame provides three entity pages: topic, person, and event. ‘Persons by Topic ’ in topic page lists up topic-centric identified experts. SPARQL query is used to retrieve them from RDF triple store through backward chaining. We gathered CiteSeer open access metadata and full text documents with the amount of about 110,000 papers. Using about 160,000 abundant topics, OntoFrame now serves topic-centric identified experts and relevant information acquired by full text analysis. 1
Studies on the syntheses of heterocyclic compounds. 657. Total synthesis of angustine, nauclefine, and gentianine (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on the syntheses of heterocyclic compounds. 657. Total synthesis of angustine, nauclefine, and gentianineTetsuji Kametani, Mitsuhiro Takeshita, Masataka Ihara, and Keiichiro FukumotoCite this: J. Org. Chem. 1976, 41, 15, 2542–2545Publication Date (Print):July 1, 1976Publication History Published online1 May 2002Published inissue 1 July 1976https://pubs.acs.org/doi/10.1021/jo00877a007https://doi.org/10.1021/jo00877a007research-articleACS PublicationsRequest reuse permissionsArticle Views259Altmetric-Citations15LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Two-year-outcomes analysis of Crohn's disease treated with rifabutin and macrolide antibiotics (1997)

Fifty-two patients with severe Crohn's disease were enrolled in this study. Six (11.5%) were intolerant of the medication and had to be excluded. The remaining 46 patients were treated with rifabutin in combination with a macrolide antibiotic (clarithromycin or azithromycin). Patients were treated for a mean of 18.7 (range 6-35) months and followed up for 25.1 (range 7-41) months. Of the 19 patients who were steroid dependent at the start of this study, only two continued to require steroids when treatment was established. A reduction in the Harvey-Bradshaw Crohn's disease activity index occurred after 6 months' treatment (P = 0.004, paired Wilcoxon test) and was maintained at 24 months (P < 0.001). An improvement in inflammatory parameters was observed as measured by a reduction in erythrocyte sedimentation rate (P = 0.009) and C-reactive protein (P = 0.03) at 18 months compared with pretreatment levels, and an increase in serum albumin at 12 months (P = 0.04). When subsets of the study population were analysed, patients with pan-intestinal disease achieved better remission at 2 years than did those with less extensive involvement (P = 0.04, Mann-Whitney U-test). No difference in treatment response by age, disease duration, the presence of granulomas on histology, or the occurrence of drug-induced side-effects, was observed. These data suggest that treatment with rifabutin and clarithromycin or azithromycin may result in a substantial clinical improvement in Crohn's disease and justify the conduct of a randomized controlled trial.
Clad through Clad: A Mechanical Water Shut Off Solution for Commingled Production from Multilayered Reservoirs (2014)

A casing or tubing clad is a metal sleeve that can be run by several intervention means into a well to isolate an area of interest, be it to provide a permanent seal over splits, holes, or perforations in tubing and casing or for water shut off. Once it is on depth, the clad or metal sleeve is expanded out against the tubing or casing wall to form a seal. Taking the example of water shut-off isolation, conventional clad technology only permits a ‘bottom up isolation’, the current technology does not have the ability to pass through an expanded clad of the same size thus it is not possible at a later date to install a second clad below an existing clad. To resolve this issue, in 2006 TOTAL instigated the development of a “Clad thru Clad” technology through a JIP (Joint Industrial Program) Water Management R&D Project with Maersk, Statoil, Chevron, and BP as partners. Meta was selected to develop the clad through clad technology. The project objective was to design an electric line conveyed clad able to pass through other similarly sized clads previously installed in a well incorporating a metal-to-metal interference fit with elastomer seal against the 5-1/2” production casing or tubing wall. In addition to the dimensional, pressure rating (5000 psi absolute, 2000 psi differential) and temperature rating (125 degC) constraints, one of the major challenges was to achieve sufficient collapse rating for the clad once expanded. The Internal Clad™ is deployed using a hydraulic expansion system. The setting tool consists of a standard electric line cable head, an electronic section (consisting of gamma ray (GR), a casing collar locator (CCL), a motor control module and pressure sensors), a down-hole reservoir, hydraulic module (consisting of motor, a pump, an intensifier and valves), and the setting tool itself. The first trial was performed in Total Indonesia’s Peciko field; well C, a gas well producing from a multilayered reservoir. The field trial was SUCCESSFUL although a number of improvements for future operations were identified.
Cache Predicting Algorithm Based on Context-Aware in Pervasive Computing (2007)

In pervasive computing, mobile device needs to make data access continuously, but in the influence of network and other factors, it could be disconnected. In order to support the continuous data access in case of disconnection, it needs to predict the possible access of data made by users and cache these data on the mobile client. Through data cache, it could store the data that the user could access in future on the client in advance, therefore the effects of disconnected mobile device on data access could be avoided and the quality of service could be accordingly raised. Among the present buffer management algorithms, the computing process either takes no account of context, or divides the data in groups to make a forecast separately according to the context information. These two methods could not fully consider the trend of the whole user access and the impact of user context on the predicted consequences. This paper puts forward a buffer management algorithm, which establishes the association between various data on the basis of data access records and makes data group; after computing the accessing frequency of data sets over the current contexts, it makes cache replacement of the results in terms of cache residence time and accessing frequency. The results of simulation tests show that this kind of algorithm could effectively improve the cache hit rate in the case of disconnected operation for handheld mobile devices, and better support the disconnected operation of mobile devices.
Hierarchical IP distribution mechanism for VANET (2010)

Vehicular Ad Hoc Network (VANET) is characterized by high speed, high efficient addressing mechanism is required for vehicles to access to Internet or communicate with each other for some practical or emergent information. The existing methods of addressing for VANET usually do not cope with instant IP address assignment and recycling. This paper proposed an AP-based centralized IP distribution system using a hierarchical Dynamic Host Configuration Protocol (DHCP) mechanism to tackle efficient addressing and timely IP recycling for reuse, and the malfunctioning APs can be detected via hierarchical mechanism. The simulation results proved that it outperformed the existing addressing mechanism.
Computer mission simulation for parametric design of undersea vehicles (1975)

The next generation of deep submersible ocean vehicles will require that more effective design approaches be utilized to achieve the full performance potential of this type of vehicle. Possible design shortcomings can be anticipated and corrected for through the exercise of a properly constructed computer program that models the salient features of the vehicle, its subsystems, the environment, and any specific operational mission requirements. A model has been developed by Sperry to assist in the design of deep submersible vehicles and their major subsystems, including sensors, propulsion unit, energy package, and vehicle structures. A variety of vehicle types - manned or unmanned, tethered or independent, small or large - can be modeled, as well as a variety of target and ocean bottom terrain features and mission profiles. The model consists of a 3700-statement Fortran V program having 34 subroutines. The program can provide an evaluation of the sensitivity of the significant vehicle performance measures to variations in design parameters. Computer generated graphs are presented to illustrate the utility of the model in several design problems including parameter determinations of propulsion system rating, weight, and size and navigation sensor accuracy requirements.
ChemInform Abstract: Microgel‐Supported Oxazaborolidines: Novel Catalysts for Enantioselective Reductions. (2000)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Comparison of sperm preparation methods. Wash and concentration, swim-up, migration-gravity sedimentation, 80% Percoll and semen filtration column. (1995)

The removal of all debris and background, as well as antifertility factors present in seminal fluid, may increase the fertilizing ability of spermatozoa. A number of semen manipulative techniques are currently available to remove the undesirable spermatozoa, debris and other factors and to increase sperm quality before performance of the various assisted reproductive technologies. We compared the impact of the wash and concentration method, swim-up method, 80% Percoll method, migration-gravity sedimentation method and SpermPrep method on postmanipulation sperm quality.
Δ<sup>9</sup>‐Fatty acid desaturase from arachidonic acid‐producing fungus (1999)

Based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cDNA and genomic gene were cloned from the fungal strain, Mortierella alpina 1S-4, which industrially produces arachidonic acid. There was a cytochrome b5-like domain linked to the carboxyl terminus of this Mortierella desaturase, as also seen in the yeast delta 9-desaturase. The Mortierella delta 9-desaturase genomic gene had only one intron, in which a novel phenomenon was observed: there was a GC-end at the 5'-terminus instead of a GT-end that is, in general, found in introns of eukaryotic genes. The full-length cDNA clone was expressed under the control of an amyB promoter in a filamentous fungus, Aspergillus oryzae, resulting in drastic changes in the fatty acid composition in the transformant cells; the contents of palmitoleic acid (16:1) and oleic acid (18:1) increased significantly, with accompanying decreases in palmitic acid (16:0) and stearic acid (18:0). These changes were controlled by the addition of maltose as a carbon source to the medium. Also, the expression of the gene caused a significant change in the lipid composition in the Aspergillus transformant. Genomic Southern blot analysis of the transformant with the Mortierella delta 9-desaturase gene as a probe confirmed the integration of this gene into the genome of A. oryzae.
Characterization of electric field-induced fusion in erythrocyte ghost membranes. (1984)

Fusion has been reported to occur in a variety of membrane systems in response to the application of certain electric currents to the medium (Zimmermann, U., 1982, Biochim. Biophys. Acta., 694:227-277). The application of a weak but continuous alternating current causes the membranes in suspension to become rearranged into the "pearl-chain" formation. Fusion can then be induced by one or more strong direct current pulses that cause pore formation. This results in the conversion of individual membranes in the "pearl-chain" formation to a single membrane with one or more hourglass constrictions that form lumens which connect the cytoplasmic compartments. As the diameter of the lumens increases, the overall membrane shape grows to one large sphere. To further characterize electric field-induced fusion, experiments were conducted using the erythrocyte ghost as a model membrane, and a new combination of electrical circuit and fusion chamber that is simpler and improved over previous systems. All odd-shaped ghosts (collapsed or partly collapsed spherical shapes, echinocytes, discocytes, and stomatocytes) in 30 mM phosphate buffer was first converted to spherocytes and then fused with increasing yields by increasing the number of pulses. After fusion, the lateral diffusion of a fluorescent lipid soluble label (Dil) from labeled to unlabeled membranes was observed to occur both with and without the appearance in phase-contrast optics of distinct communication (lumens) between cytoplasmic compartments of the fused membranes. Connections between cytoplasmic compartments, however, were unmistakable with the instant transfer of a fluorescent water-soluble label (fluorescein isothiocyanate-dextran) from labeled to unlabeled cytoplasmic compartments upon fusion. Although pulses still resulted in the lateral diffusion of Dil to unlabeled membranes, the presence of glycerol in the medium strongly reduced the yield of lumens observable by phase-contrast optics in fusion events. The presence of glycerol also inhibited the conversion of membranes to spherocytes, but did not inhibit the lateral diffusion of Dil from labeled to unlabeled membranes.
Dinitrogen Complexes Supported by Tris(phosphino)silyl Ligands (2009)

The tetradentate tris(phosphino)silyl ligand [SiP(iPr)(3)] ([SiP(iPr)(3)] = [Si(o-C(6)H(4)P(i)Pr(2))(3)](-)) has been prepared, and its complexation with iron, cobalt, nickel, and iridium precursors has been explored. Several coordination complexes have been thoroughly characterized and are described. These include, for example, the divalent trigonal bipyramidal metal chlorides [SiP(iPr)(3)]M-Cl (M = Fe, Co, Ni), as well as the monovalent dinitrogen adducts [SiP(iPr)(3)]M-N(2) (M = Fe, Co, Ir), which are compared with related [SiP(Ph)(3)]M-Cl and [SiP(Ph)(3)]M-N(2) species (M = Fe, Co). Complexes of this type represent the first examples of terminal dinitrogen adducts of monovalent iron, and the ligand architecture allows examination of a unique class of dinitrogen adducts with a trans-disposed silyl donor. Oxidation of the appropriate [SiP(R)(3)]M-N(2) precursors affords the divalent iron triflate [SiP(Ph)(3)]Fe(OTf) and trivalent cobalt triflate {[SiP(iPr)(3)]Co(OTf)}{OTf} complexes, which are of interest for group transfer studies because of the presence of a labile triflate ligand. Comparative electrochemical, structural, and spectroscopic data are provided for these complexes.
Purification and Properties of a Folate-catabolizing Enzyme (2000)

We have identified and purified to homogeneity an enzyme from rat liver that catalyzes the oxidative catabolism of 5-formyltetrahydrofolate to p-aminobenzoylglutamate and a pterin derivative. Purification of the enzyme utilized six column matrices, including a pterin-6-carboxylic acid affinity column. Treatment of crude rat liver extracts with EDTA or heat decreased the specific activity of the enzyme by up to 85%. Peptides generated from the purified protein were sequenced and found to be identical to primary sequences present within rat light chain or heavy chain ferritin. Commercial rat ferritin did not display catabolic activity, but activity could be acquired with iron loading. The purified enzyme contained 2000 atoms of iron/ferritin 24-mer and displayed similar electrophoretic properties as commercial rat liver ferritin. The ferritin-catalyzed reaction displayed burst kinetics, and the enzyme catalyzed only a single turnover in vitro. Expression of rat heavy chain ferritin cDNA resulted in increased rates of folate turnover in cultured Chinese hamster ovary cells and human mammary carcinoma cells and reduced intracellular folate concentrations in Chinese hamster ovary cells. These results indicate that ferritin catalyzes folate turnover in vitro and in vivo and may be an important factor in regulating intracellular folate concentrations.