gebhart/patrepeval huggingface dataset:
<i>Drosophila</i> Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules (2002)

Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.
Stable transduction of quiescent CD34<sup>+</sup>CD38<sup>−</sup>human hematopoietic cells by HIV-1-based lentiviral vectors (1999)

We compared the efficiency of transduction by an HIV-1-based lentiviral vector to that by a Moloney murine leukemia virus (MLV) retroviral vector, using stringent in vitro assays of primitive, quiescent human hematopoietic progenitor cells. Each construct contained the enhanced green fluorescent protein (GFP) as a reporter gene. The lentiviral vector, but not the MLV vector, expressed GFP in nondivided CD34(+) cells (45.5% GFP+) and in CD34(+)CD38(-) cells in G0 (12.4% GFP+), 48 hr after transduction. However, GFP could also be detected short-term in CD34(+) cells transduced with a lentiviral vector that contained a mutated integrase gene. The level of stable transduction from integrated vector was determined after extended long-term bone marrow culture. Both MLV vectors and lentiviral vectors efficiently transduced cytokine-stimulated CD34(+) cells. The MLV vector did not transduce more primitive, quiescent CD34(+)CD38(-) cells (n = 8). In contrast, stable transduction of CD34(+)CD38(-) cells by the lentiviral vector was seen for over 15 weeks of extended long-term culture (9.2 +/- 5.2%, n = 7). GFP expression in clones from single CD34(+)CD38(-) cells confirmed efficient, stable lentiviral transduction in 29% of early and late-proliferating cells. In the absence of growth factors during transduction, only the lentiviral vector was able to transduce CD34(+) and CD34(+)CD38(-) cells (13.5 +/- 2.5%, n = 11 and 12.2 +/- 9.7%, n = 4, respectively). The lentiviral vector is clearly superior to the MLV vector for transduction of quiescent, primitive human hematopoietic progenitor cells and may provide therapeutically useful levels of gene transfer into human hematopoietic stem cells.
An Approach to the Classification of Unknown Biological Agents with Cell Based Sensors (1998)

channels; and the fi nal repolarization due to the closure of Ca 2+ The broad-spectrum sensitivity of cell based biosensors offers channels and the prolonged opening of K + channels [6]. The the capability for detecting previously unknown biological agents.
Energy-Aware Mobile Edge Computing for Low-Latency Visual Data Processing (2017)

New opportunities exist for applications such as disaster incident response that can benefit from the convergence of Internet of Things (IoT) and cloud computing technologies. Particularly, new paradigms such as Mobile Edge Computing (MEC) are becoming feasible to handle the data deluge occurring in the network edge to gain insights that assist in real-time decision making. In this paper, we study the potential of MEC to address application issues related to energy management on constrained IoT devices with limited power sources, while also providing low-latency processing of visual data being generated at high resolutions. Using a face recognition application that is important in disaster incident response scenarios, we analyze the tradeoffs in computing policies that offload visual data processing (i.e., to an edge cloud or a core cloud) at low-to-high workloads, and their impact on energy consumption under different visual data consumption requirements (i.e., users with thick clients or thin clients). From our empirical results obtained from experiments with our face recognition application on a realistic edge and core cloud testbed, we show how MEC can provide flexibility to users who desire energy conservation over low-latency or vice versa in the visual data processing.
Reduced Steady State Heating and Air Conditioning Loads via Reduced Glazing Thermal Conductivity (2011)

<div class="section abstract"><div class="htmlview paragraph">Heat transfer between the ambient and the air in a vehicle cabin determines the nominal steady state load on the vehicle's heating, ventilation and air conditioning (HVAC) system, a significant factor for vehicle efficiency and greenhouse gas emissions. This paper highlights the effect of glazing (i.e. window) thermal conductivity on steady state heat transfer, with high and low thermal conductivities represented respectively by monolithic glass and standard polycarbonate. Computational fluid dynamics simulations are summarized for a model car cabin including HVAC vents, interior seating, and a rooflite. Passenger and moisture effects are not included. Monthly temperature and radiation data for Phoenix, Arizona and Minneapolis, Minnesota are used to define hot and cold climate scenarios. The simulations yield the heat transfer and its sensitivity to glazing thermal conductivity and ambient conditions, for both stationary and moving cars, when cabin air is maintained at a comfortable temperature. The results show that the inherently lower thermal conductivity of polycarbonate relative to glass, with common optical transmission parameters, affords reduced steady state heat transfer, and thus reduced nominal HVAC load, in all of the climate and state of motion scenarios. Summarized for selected scenarios and glazing locations are breakdowns of the heat transfer into radiation and convection components, and the average inside glazing surface temperature. The latter is found to be closer to the steady state cabin air temperature for polycarbonate than for glass, in both hot and cold climates.</div></div>
Dissection of a class II tRNA synthetase: Determinants for minihelix recognition are tightly associated with domain for amino acid activation (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleDissection of a class II tRNA synthetase: Determinants for minihelix recognition are tightly associated with domain for amino acid activationDouglas D. Buechter and Paul SchimmelCite this: Biochemistry 1993, 32, 19, 5267–5272Publication Date (Print):May 18, 1993Publication History Published online1 May 2002Published inissue 18 May 1993https://pubs.acs.org/doi/10.1021/bi00070a039https://doi.org/10.1021/bi00070a039research-articleACS PublicationsRequest reuse permissionsArticle Views109Altmetric-Citations23LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Development of Vi conjugate – a new generation of typhoid vaccine (2013)

Typhoid fever remains to be a serious disease burden worldwide with an estimated annual incidence about 20 million. The licensed vaccines showed moderate protections and have multiple deficiencies. Most important of all, none of the licensed typhoid vaccines demonstrated protection for children under 5 years old. These limitations impeded successful implementation of typhoid vaccination programs. To improve immunogenicity Vi was conjugated to rEPA, a recombinant exoprotein A from Pseudomonas aeruginosa. Vi-rEPA showed higher and longer lasting anti-Vi IgG in adults and children than Vi alone in high endemic areas. In school-age children and adults, the immunity persisted more than 8 years. In a double-blind, placebo-controlled and randomized efficacy trial in 2- to 5-year-old children, Vi-rEPA conferred 89% protective efficacy against typhoid fever and the protection lasted at least 4 years. When given concomitantly with infant routine vaccines, Vi-rEPA was safe, immunogenic and showed no interference with the routine vaccines. Vi conjugate vaccine was also attempted and successfully demonstrated by several other laboratories and manufactures. Using either rEPA or different carrier proteins, such as diphtheria or tetanus toxoid, recombinant diphtheria toxin (CRM197), the Vi conjugates synthesized was significantly more immunogenic than Vi alone. Recently, two Vi-tetanus toxoid conjugates were licensed in India for all ages, starts as young as 3 month old. This new generation of typhoid vaccine opens up a new era for typhoid prevention and elimination.
Novel anti‐inflammatory chalcone derivatives inhibit the induction of nitric oxide synthase and cyclooxygenase‐2 in mouse peritoneal macrophages (1999)

In a previous work, we tested a series of chalcone derivatives as possible anti-inflammatory compounds. We now investigate the effects of three of those compounds, CHI, CH8 and CH12, on nitric oxide and prostanoid generation in mouse peritoneal macrophages stimulated with lipopolysaccharide and in the mouse air pouch injected with zymosan, where they showed a dose-dependent inhibition with inhibitory concentration 50% values in the microM range. This effect was not the consequence of a direct inhibitory action on enzyme activities. Our results demonstrated that chalcone derivatives inhibited de novo inducible nitric oxide synthase and cyclooxygenase-2 synthesis, being a novel therapeutic approach for inflammatory diseases.
Achieving significantly enhanced visible-light photocatalytic efficiency using a polyelectrolyte: the composites of exfoliated titania nanosheets, graphene, and poly(diallyl-dimethyl-ammonium chloride) (2015)

A high-performance visible-light-active photocatalyst is prepared using the polyelectrolyte/exfoliated titania nanosheet/graphene oxide (GO) precursor by flocculation followed by calcination. The polyelectrolyte poly(diallyl-dimethyl-ammonium chloride) serves not only as an effective binder to precipitate GO and titania nanosheets, but also boosts the overall performance of the catalyst significantly. Unlike most titania nanosheet-based catalysts reported in the literature, the composite absorbs light in the UV-Vis-NIR range. Its decomposition rate of methylene blue is 98% under visible light. This novel strategy of using a polymer to enhance the catalytic performance of titania nanosheet-based catalysts affords immense potential in designing and fabricating next-generation photocatalysts with high efficiency.
Predictive digital filtering of sinusoidal signals (1998)

The p-step ahead predictive filters for narrowband waveforms with m distinct spectral peaks are considered. By minimization of the noise gain, the coefficients of the optimal Lth order FIR predictor is derived, where L>2m-1. The minimum-length FIR predictor is given by L=2m-1, and the feedback extension of this predictor is studied. Design of feedback gains subject to different optimization criteria is studied in detail. The generalization to complex-valued signals, cascaded predictors, and adaptive predictors are also included. Several design and simulation examples are presented.
Formaldehyde scavenging from peritoneal dialysis solutions using reduced aminothiol compounds (2004)

Scavenging with aminothiol compounds masked the destructive carbonyl group (C = O) of formaldehyde and formed a compound that has antioxidant properties. The addition of aminothiol compounds may improve the biocompatibility of commercial PD solutions.
Cannulation of simple bone cysts (2002)

We describe a consecutive series of 26 patients with simple bone cysts who were treated by curettage, multiple drilling and continuous decompression by the insertion of either a cannulated screw or a pin. In the first 15 patients we used titanium cannulated screws (group 1) and in the next 11 a cannulated hydroxyapatite pin (group 2). Satisfactory healing was achieved in 12 patients in group 1 (80%) and in all in group 2. This technique seems to be a promising option for the treatment of simple bone cysts. The cannulated hydroxyapatite pin is recommended because of its higher success rate and the fact that it does not need to be removed.
Vein authentication using color information and image matching with high performance on natural light (2009)

In the past, we have made use of infrared CCD camera to take pictures of vein for vein authentication. However, mounting infrared CCD camera for electrical device is dangerous due to the problem of privacy invasions. In addition, there have been registration problem which lower the correlation. Then, we propose a method that visualizes vein using color information subpixel level image registration technique. These methods do not require infrared CCD camera, and furthermore. Consequently, we create a high-accuracy authentication system without the use of infrared CCD camera.
Drake's Drum' (1940)

Journal Article Drake's Drum' Get access Harold S. Rogers Harold S. Rogers 1Oxford Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume 178, Issue 18, 4 May 1940, Page 318, https://doi.org/10.1093/nq/178.18.318b Published: 04 May 1940
Large-bandwidth flat cylindrical array with circular polarisation and omnidirectional radiation (1979)

Experimental properties of a flat cylindrical array with circular polarisation and omnidirectional radiation are presented. The thickness of the array wrapped on a perfect conducting cylinder is 0.04λ0. A bandwidth of 10% has been obtained. It is now possible to decrease the thickness by a factor 2 (i.e. to 0.02λ0), when using a short-circuited elementary source instead of a flat folded dipole, and yet retain the same band-width.
A Shape Index from Molecular Graphs (1985)

Abstract A numerical index of molecular shape has been derived from the graph of the non‐hydrogen molecular skeleton. The index is based on the count of 2‐bond fragments in a graph relative to the maximum number possible in the isomeric star graph and the minimum number in the isomeric linear graph. In this initial study all non‐hydrogen atoms and all bonds are considered equal. The 2 κ index describes the molecular shape in relationship to the star and linear graph and is normalized to the number of atoms. Applications are presented for shape similarity estimation, cavity filling ability and QSAR analyses.
Small Pore Aluminum Phosphate Molecular Sieves with Chabazite Structure: Incorporation of Cobalt in the Structures -34 and -44 (1995)

CoAPO and CoSAPO with -34 and -44 framework structures have been synthesized. The presence of cobalt in the framework has been established by a number of experimental techniques. Distribution of cobalt in the crystal was heterogeneous for the CoAPSOs. Using electron microprobe analysis, it was observed that cobalt is homogeneously distributed in the CoAPOs while in the CoSAPOs it has higher concentration near the center of the crystal that progressively decreases toward the edges.
Sequence diversity in the major fimbrial subunit gene (<i>flp‐1</i>) of <i>Actinobacillus actinomycetemcomitans</i> (2002)

Cells of the periodontal pathogen Actinobacillus actinomycetemcomitans exhibit tight adherence to surfaces such as glass, plastic and hydroxyapatite, a property that probably plays an important role in the ability of this bacterium to colonize teeth and other surfaces. Tight adherence is mediated by long fibrils of bundled pili (fimbriae) that form on the surface of the cell. The flp-1 gene encodes the major pilin protein component of A. actinomycetemcomitans fimbriae. In this study we compared flp-1 DNA sequences from 43 strains of A. actinomycetemcomitans isolated in Europe, Japan and the United States and identified seven distinct flp-1 allelic classes. DNA and predicted protein sequences were almost completely conserved within each flp-1 class but were highly divergent between classes. Most amino acid substitutions occurred in the C-terminus of the pilin protein, a region that has been shown to be important for the bundling and adhesive properties of the pili. flp-1 classes correlated with serotypes and 16S rRNA genotypes in most strains. At least five strains showed evidence of horizontal transfer of flp-1 between strains of different serotypes and 16S rRNA genotypes. Four of the seven flp-1 classes were present in geographically diverse isolates. Strains representing all seven flp-1 classes, but not a strain carrying a transposon insertion in flp-1, bound avidly to polystyrene in an in vitro adherence assay. Strains representing six of the seven flp-1 classes were isolated from localized juvenile periodontitis patients, suggesting that phylogenetically diverse strains carry pathogenic potential. Our findings provide a framework for future biochemical, immunological and genetic studies of A. actinomycetemcomitans fimbriae.
Secretory Phospholipase A2 Receptor-Mediated Activation of Cytosolic Phospholipase A2 in Murine Bone Marrow-Derived Mast Cells (2000)

The current study examined the signal transduction steps involved in the selective release of arachidonic acid (AA) induced by the addition of secretory phospholipase A2 (sPLA2) isotypes to bone marrow-derived mast cells (BMMC). Overexpression of sPLA2 receptors caused a marked increase in AA and PGD2 release after stimulation of BMMC, implicating sPLA2 receptors in this process. The hypothesis that the release of AA by sPLA2 involved activation of cytosolic PLA2 (cPLA2) was next tested. Addition of group IB PLA2 to BMMC caused a transient increase in cPLA2 activity and translocation of this activity to membrane fractions. Western analyses revealed that these changes in cPLA2 were accompanied by a time-dependent gel shift of cPLA2 induced by phosphorylation of cPLA2 at various sites. A noncatalytic ligand of the sPLA2 receptor, p-amino-phenyl-alpha-D-mannopyranoside BSA, also induced an increase in cPLA2 activity in BMMC. sPLA2 receptor ligands induced the phosphorylation of p44/p42 mitogen-activated protein kinase. Additionally, an inhibitor of p44/p42 mitogen-activated protein kinase (PD98059) significantly inhibited sPLA2-induced cPLA2 activation and AA release. sPLA2 receptor ligands also increased Ras activation while an inhibitor of tyrosine phosphorylation (herbimycin) inhibited the increase in cPLA2 activation and AA release. Addition of partially purified sPLA2 from BMMC enhanced cPLA2 activity and AA release. Similarly, overexpression of mouse groups IIA or V PLA2 in BMMC induced an increase in AA release. These data suggest that sPLA2 mediate the selective release of AA by binding to cell surface receptors and then inducing signal transduction events that lead to cPLA2 activation.
Hydrophilic analogs of (<i>R</i>,<i>R</i>)-diaminocyclohexane dichloroplatinum (DACH) and the influence of relative stereochemistry on antitumor activity (1993)

A series of analogs of (R,R)-1,2-diaminocyclohexane dichloroplatinum(II) (DACH) containing stereochemically defined hydroxy groups and appropriate acidic leaving groups were synthesized and tested as antitumor agents. The (1α,2β,3α,4β)-1,4-dihydroxy-2,3-diaminocyclohexane analog showed the highest potency against P388 leukemia in mice. The results indicate that increasing the hydrophilicity of the platinum complex to a certain extent could improve the antitumor activity of the drug. It is also likely that the stereochemical disposition of the substituents on the cyclohexane ring affects the antitumor activity.
GroEL Heat Shock Protein of <i>Haemophilus ducreyi</i> : Association with Cell Surface and Capacity To Bind to Eukaryotic Cells (1998)

The Haemophilus ducreyi homolog of GroEL, a 58.5-kDa heat shock protein (Hsp), is a dominant protein produced not only in response to heat stress but also under in vitro growth conditions. Extracellular localization of the 58.5-kDa Hsp was investigated by whole-cell enzyme-linked immunosorbent assay (ELISA) and immunoelectron microscopy and in supernatants of washed bacteria by immunoblotting with a Haemophilus ducreyi GroEL-specific mouse monoclonal antibody (BB11). To investigate binding of the Hsp to eukaryotic cells, the 58.5-kDa Hsp was purified by ion-exchange and size exclusion chromatography; incubated with HEp-2 cells, HeLa cells, and human fibroblasts; and then analyzed by immunoblotting. Direct involvement of the 58.5-kDa Hsp in the adherence of H. ducreyi to HEp-2 cells was investigated by using an inhibition assay. An epitope of the 58.5-kDa Hsp was detected by whole-cell ELISA on all of the strains tested, suggesting that it is associated with the cell surface. This was also supported by immunoelectron microscopy results. In supernatants of washed bacteria, the 58.5-kDa Hsp was detected by immunoblotting after 10 h of cultivation. The 58.5-kDa Hsp bound to the eukaryotic cells tested but exerted only limited (about 20%) inhibition of H. ducreyi adherence to HEp-2 cells. These results demonstrate that the 58.5-kDa Hsp of H. ducreyi is associated with the bacterial surface, binds to eukaryotic cells, and partially influences H. ducreyi adherence to HEp-2 cells, indicating possible involvement of the 58.5-kDa Hsp in the attachment of bacteria to host cells and to each other.
Synthesis and Antiviral Bioactivity of Chiral Thioureas Containing Leucine and Phosphonate Moieties (2010)

A series of novel chiral thioureas 3a-n bearing leucine and phosphonate moieties were synthesized in excellent yields. The structures of the compounds were completely characterized by elemental analysis, IR, 1H-, 13C-, 31P- and 19F-NMR spectral data. A half-leaf method was used to determine the in vivo protective and curative efficacies of the title products against tobacco mosaic virus (TMV). The compounds 3l and 3n displayed good in vivo protection and curative effects against TMV with inhibitory rates of 60.1, 62.8% (protection) and 56.7, 53.6% (curative) at 0.5 mg/mL, respectively. To the best of our knowledge, this is the first report on the antiviral activity of chiral thioureas containing leucine and phosphonate moieties.
An evaluation of the noise performance of Fe, Co, Si, and B amorphous alloys in ring-core fluxgate magnetometers (1985)

The work described herein has addressed two questions. Can amorphous metal alloys be used as core material for high-performance fluxgate magnetometers, and do these alloys provide a means for further understanding the noise mechanism(s) of fluxgate magnetometers?We have prepared and studied a suite of fourteen alloys of content (Fe, Co) 70 , (Si, B) 30 . Noise levels of the as-cast alloys correlate with coercivity and hysteresis losses and have a local minimum at zero magnetostriction. However, our overall noise minimum coincides with the minimum measured core losses—not at zero magnetostriction.Our best cores perform favourably when compared with the best commercially available Permalloy cores and outperform all cores of the same dimensions that we have examined. Thus we are able to conclude that amorphous metal alloys are a likely source of high-quality material for fluxgate magnetometers.