gebhart/patrepeval huggingface dataset:
Laminin peptide‐conjugated chitosan membrane: Application for keratinocyte delivery in wounded skin (2006)

Tissue engineering requires the delivery and survival of cells to organ sites needing repair. Previously, we showed that an active laminin peptide (AG73: RKR-LQVQLSIRT)-conjugated chitosan membrane promoted cell adhesion and spreading in vitro. Here, we seeded human keratinocytes onto AG73-chitosan membranes and found that nearly 80% of the cells were attached to the membranes within 2 h. The membranes carrying the keratinocytes were inverted and placed onto exposed muscle fascia on the backs of nude mice. After 3 days, the keratinocytes had migrated from the membrane and established a stratified epidermis-like structure on the fascia. Cells recognize the AG73 through transmembrane proteoglycan syndecans, which recognition system has not previously been tested in tissue engineering applications. We suggest that the AG73-chitosan membrane is useful as a therapeutic formulation and is applicable as a cell delivery system such as delivering keratinocytes to a wound bed.
Routing bandwidth model for feed forward neural networks on multicore neuromorphic architectures (2013)

Specialized multi-core architectures can provide significant speedups for neural network applications. In this study, we examined the on-chip routing network bandwidth requirements for such architectures processing large multi-layered feed forward neural networks in a pipelined manner. Two on-chip routing network topologies were examined: mesh networks and hybrid bus-mesh networks. Two routing bandwidth models were developed for each network topology: one examined sending neuron outputs from one layer to the next, while the other examined the streaming of synaptic weights from off-chip memory. The model was validated through several simulations studies. For both mesh and bus-mesh interconnection area and power of the on-chip routing network was estimated using the Orion on-chip network tool. Our results show that in multi-core neuromorphic architectures, a bus-mesh interconnection requires less routing area and power compared to a mesh interconnection. We also observed that the accumulated bandwidth requirement in the on-chip network to access off-chip data is much greater than bandwidth required to send neuron outputs between cores.
Induction of IL-8 expression in T cells uses the CD28 costimulatory pathway. (1994)

IL-8, a potent chemotactic factor for neutrophil granulocytes and lymphocytes, is a proinflammatory cytokine secreted by a variety of cell types, including T cells. Stimulation of the CD28 cell surface molecule delivers costimulatory signals essential for lymphokine production in activated T cells via a conserved sequence element found in the promoter of several lymphokine genes. Anti-CD28-stimulated T cells produced significant amounts of IL-8; additionally, costimulation with anti-CD3 and anti-CD28 Abs resulted in a synergistic induction of IL-8 secretion. Sequence homology, single nucleotide mutations, and anti-CD28 Ab stimulation studies established that the NF-kappa B-like sequence in the promoter of the IL-8 gene functioned as a CD28 response element. Furthermore, cyclosporin A, but not rapamycin, blocked the synergistic induction of IL-8 expression achieved with anti-CD3 and anti-CD28 costimulation. The involvement of a CD28 response element in the induction of IL-8 expression in activated T cells may provide new insights into the pathogenesis and persistence of immune disorders characterized by increased levels of IL-8, such as psoriasis and rheumatoid arthritis.
Modified taxols. 2. Oxidation products of taxol (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTModified taxols. 2. Oxidation products of taxolNeal F. Magri and David G. I. KingstonCite this: J. Org. Chem. 1986, 51, 6, 797–802Publication Date (Print):March 1, 1986Publication History Published online1 May 2002Published inissue 1 March 1986https://pubs.acs.org/doi/10.1021/jo00356a006https://doi.org/10.1021/jo00356a006research-articleACS PublicationsRequest reuse permissionsArticle Views470Altmetric-Citations48LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
EFFECTS OF EURO-COLLINS, UNIVERSITY OF WISCONSIN, AND NEW EXTRACELLULAR-TYPE TREHALASE-CONTAINING KYOTO SOLUTIONS IN AN EX VIVO RAT LUNG PRESERVATION MODEL (1996)

The postpreservation pulmonary functions with the new ET-K solution were better than those with the EC solution, and were equal to those with the UW solution. This new solution is expected to contribute to the increase in donor lungs for clinical lung transplantation. In addition, this ex vivo rat reperfusion model is simple and highly reliable, and can be widely used in the studies of pulmonary preservation.
Detection of Chlamydia trachomatis Antigens by Enzyme Immunoassay and Immunofluorescence in Genital Specimens from Symptomatic and Asymptomatic Men and Women (1986)

Chlamydia trachomatis antigens were detected in populations with the following infection prevalences: 26.5% (36 of 136) of men and 27.7% (48 of 173) of women attending a sexually transmitted disease clinic, 16.3% (53 of 324) of women attending a Planned Parenthood clinic, and 3.4% (4 of 117) of an obstetrics and gynecologic practice. Compared with cell culture of the combined female cervical specimens (15.8% prevalence), the respective sensitivities of Chlamydiazyme (Abbott Laboratories, North Chicago, Illinois) and Microtrak (Syva, Palo Alto, California) were 98.3% and 87.9%, specificities were 97.5% and 98.4%, positive predictive values were 87.7% and 92.7%, and negative predictive values were 99.7% and 97.5%. Both assays were 70.0% sensitive with male urethral specimens, and the other parameters of performance ranged between 84.0% and 97.2%. The antigen detection assays, compared with culture, performed equally well in subjects without or with clinical signs.
Network-aware coordination of virtual machine migrations in enterprise data centers and clouds (2013)

Virtual machine(VM) migration usually requires a considerable amount of system resources such as the network bandwidth. In the case of multiple simultaneous migrations, such resource demands will increase dramatically and are difficult to be satisfied immediately. This paper proposes a scheduling method for multiple VM migrations to guarantee the fast completion of those tasks and hence the reduced impacts on system performance. We discover the best bandwidth sharing policy for each network link, and further propose a bin-packing algorithm to organize bandwidth resources from all the network links. As a result, the migration tasks can fully utilize available resources in the whole network to achieve the fast completion.
Protein adsorption and endothelial cell attachment and proliferation on PAPI‐based additive modified poly(ether urethane ureas) (1993)

To better understand vascular interactions with poly(ether urethane urea) (PEUU) materials, protein adsorption, and endothelial cell attachment and proliferation assays were performed on a base PEUU formulation, on PEUU formulations loaded with hydrophobic and amphiphilic poly(methylene-[polyphenyl isocyanate]) (PAPI) based additives, and on PEUU formulations in which some of the polymer chains had been endcapped with either diisopropylaminoethyl (DIPAA) or decyl (DA) moieties. Protein adsorption experiments with PAPI-based additives showed that additive loaded PEUU formulations adsorbed significantly lower amounts of the studied proteins than did the unloaded PEUU. Protein adsorption to the DA and DIPAA endcapped PEUU films was found not to vary consistently from that of the unloaded PEUU film. Endothelial cell attachment and proliferation experiments with PAPI-DA and polyethylene glycol-PAPI-DA (PEG-PAPI-DA) loaded PEUU films showed that many of the films exhibited attachment and proliferation that was significantly enhanced compared to PEUU A' and that approached or equaled that of the tissue culture polystyrene control. Experiments with PAPI-DIPAA and PEG-PAPI-DIPAA loaded PEUU films exhibited attachment and proliferation data that was often below 10% of the tissue culture polystyrene control values. Experiments with the DA and DIPAA endcapped PEUU films showed endothelial cell attachment and proliferation that was statistically indistinguishable from the PEUU A' values. Contact angle analysis was carried out on the endcapped PEUU films, on the PAPI-based additive loaded PEUU films, and on PEUU A' using the sessile drop method. The advancing and receding contact angle behavior of the PAPI-based additive loaded PEUU films deviated markedly from the behavior of PEUU A', suggesting that the additives were present at the PEUU-water interface. The contact angle behavior of the endcapped PEUUs was similar to that of PEUU A', suggesting that the DA and DIPAA endcap moieties did not exist at the hydrated PEUU surface in appreciable quantities. To explain the differences in protein adsorption and endothelial cell behavior on the air side of additive loaded PEUUs when compared to the base PEUU, it was assumed that the additives near this region of the solvent swollen PEUU matrix may have migrated to, at, or near the PEUU-air interface during film formation, creating an additive enriched PEUU surface region.(ABSTRACT TRUNCATED AT 400 WORDS)
Isolation and characterization of the QM promoter (1996)

This report describes the isolation, sequencing and preliminary characterization of the first 1 kb of the 5'-regulatory region of the human QM gene. This region and the 5' -half of the transcribed region of the QM gene are enriched for C and G nucleotides with no bias against CpG dinucleotides--indicative of a CpG island. Several consensus GC boxes are present within the sequence. Most are clustered at the distal end, with one site present in the proximal 200 bp of the promoter. Electrophoretic mobility shift experiments and luciferase assays done in insect cells transfected with an Sp1 expression construct suggest that most of these sites can bind Sp1 or a closely related factor. In addition, the promoter is shown to be responsive to cAMP via a response element (CRE) in the proximal promoter. Studies with 5'-end and internal deletion mutants suggest that elements in the distal promoter exert their positive effect through interactions with a proximal element(s). Candidate proximal elements include the proximal GC box and a 43 bp region between a KpnI site (at -182) and a Smal site (at -139).
Design of transceiver circuits for NRZ signaling in inductive inter-chip wireless superconnect (2005)

Recently, a 3-dimensional integrated circuits (3D-ICs) are appeared for high density packaging. High speed interconnection is expected in 3D-ICs. A wireless bus for stacked chips is presented in this paper. The interface utilizes inductive coupling with metal spiral inductors. Transceiver circuits for non-return-to-zero (NRZ) signaling were developed to reduce the power and achieve high data rate. Receiver circuit's timing margin and sensitivity is discussed. Test chip was fabricated and timing margin was measured. The result agrees very well with simulation. Then crosstalk reduction techniques for channel array are proposed and its efficiency is demonstrated by test chip. The chip has 3/spl times/65 channel array, each channel's performance is 1 Gbps, and maximum data rate of 195 Gbps are achieved by using proposed cross talk reduction techniques.
Effects of Yeast Hydrolysate on Hepatic Lipid Metabolism in High-Fat-Diet-Induced Obese Mice: Yeast Hydrolysate Suppresses Body Fat Accumulation by Attenuating Fatty Acid Synthesis (2012)

YH supplementation suppressed body fat accumulation by attenuating fatty acid synthesis through the downregulation of hepatic G6PD and ME activities.
Continuously Microscopically Observed and Process-Controlled Cell Culture Within the<i>SlideReactor:</i>Proof of a New Concept for Cell Characterization (2007)

Certain cell types, especially primary human cells, favor a well-defined culture environment offering continuous supply of nutrients and oxygen and waste product removal. Several bioreactors based on special matrices or hollow fibers have been developed that provide such conditions. However, characterization of matrix re-organization or growth of tissue within these systems is possible only after culture termination. Evaluation of the influence of certain medium additives or culture conditions (e.g., temperature, oxygenation) on cell viability, expansion, and differentiation within these systems remains a challenging task. The SlideReactor, a miniaturized hollow fiber–based bioreactor, was developed to enable the observation of cells during culture. An operation concept offering predefined conditions for various cell types has been designed. For proof of concept, primary human cells (hepatocytes, fibroblasts, keratinocytes) and cell lines (HepG2, HuH7, C3A, WiDr, SkHep1) were cultured and observed. A series of experiments (n = 40) showed the feasibility of the set-up; determination of process parameters and continuous observation is possible. The SlideReactor may serve as a simple and cost-efficient tool for cell characterization and optimization of cell-culture conditions.
Intracellular citrate or externally applied tetraethylammonium ions produce calcium‐dependent action potentials in an insect motoneurone cell body (1979)

1. Electrophysiological observations have been made upon the cell body of an identified motoneurone of the cockroach, Periplaneta americana. Normal responses were compared with those observed after intracellular injection of citrate anions or when the preparation was bathed in solutions containing tetraethylammonium ions (TEA+).2. Normally when depolarized, the motoneurone soma gave a series of damped oscillations; the amplitude of these responses increased with increase in the applied current.3. After citrate ions had been injected into the neurone soma, all-or-none action potentials were evoked by depolarization; such responses appeared about 5-10 min after the onset of citrate injection. Injection of EGTA produced similar effects. Citrate and EGTA probably produce their effect through a reduction in the intracellular free calcium concentration.4. When preparations were bathed in saline solution containing 50 mM-TEA+, soma depolarization produced prolonged all-or-none action potentials (up to approximately 100 msec duration).5. The action potentials produced by citrate injection or externally applied TEA+ appeared to have a similar ionic mechanism; they were not depressed by sodium-free solutions or by tetrodotoxin (4 x 10(-6)M) but were reversibly blocked in saline solution containing 40 mM-manganous chloride.6. The overshoot amplitude of action potentials recorded after injection of citrate anions or in solutions containing TEA+ showed a 22.5 mV change for a ten-fold change in the external calcium concentration.7. Both intracellular citrate and external TEA+ caused a significant increase in the input resistance and membrane time constant of the motoneurone.8. It is concluded that action potentials generated under various experimental conditions in the soma of this insect motoneurone map have differing ionic mechanisms.
Assessment of Multipath TCP (MPTCP) Protocol on Lebanese Access Networks (2018)

Multipath TCP protocol (MPTCP) is an evolution of TCP protocol that can effectively use multiple paths within a single transport connection. It is implemented in the kernel of devices operating system (OS) and is transparent for applications. It has different implementations for Linux, Android and iOS. In this paper, we assess the performance, in terms of throughput, obtained by using MPTCP protocol over Lebanese access networks. We consider ADSL and mobile connections. We show that the MPTCP protocol allows to aggregate the throughput of both ADSL and 4G connections when using Alfa operator, while Touch does not permit the use of MPTCP protocol. With MPTCP, the client can benefit from the summation of throughputs on ADSL and LTE connections. Furthermore, if one connection fails on a link, the connection pursue without any interruption on the other links. Thanks to MPTCP, a Lebanese client/company can benefit from a good internet connection with a throughput of up to 90 Mpbs by aggregating several moderate connections.
Flow cytometric detection of most proteins in the cell surface proteome is unaffected by trypsin treatment (2018)

Flow cytometry is often performed on adherent cells or solid tissues that have been released from their growth substrate or disaggregated by enzymatic digestion. Although detection of strongly expressed cell surface proteins following such procedures indicates that many survive treatment with proteolytic enzymes, applications such as cell surface proteomics involve assessment of the expression of more than 200 proteins and it is important to know how to interpret negative results. To address this problem, we performed flow cytometry-based cell surface proteomic analysis on two non-adherent cell lines, THP1 and K562, after mock and authentic trypsin treatment, according to a widely used protocol to remove adherent cells (0.25% trypsin, 2.21 mM EDTA, 37°C, 5 min). In a single screening experiment, we examined the effect of treatment on mean fluorescence intensity and on the percent of positive cells and determined the false negative rate. Of 164 determinations that were ≥20% positive after mock treatment, 13 (7.9%) were <20% positive after trypsin treatment. Four proteins were chosen for time-course studies (performed in triplicate), confirming initial sensitivity results but revealing significant variability in the magnitude of the trypsin effect. When trypsin sensitivity of individual proteins was examined as a function of the number of predicted high probability extracellular trypsin cleavage sites, we found that the markers that yielded false negatives all had high numbers of sites (>30), but even so, the majority of proteins with high numbers of trypsin sites could still be detected after mild trypsin treatment. We conclude that the great majority of cell surface proteins can be detected after mild trypsin treatment, but that negative results should not be over-interpreted, due to the possibility of false negatives.
Molecular Cloning and Expression of Mouse GD1α/GT1aα/GQ1bα Synthase (ST6GalNAc VI) Gene (2000)

A novel member of the mouse CMP-NeuAc:beta-N-acetylgalactosaminide alpha2,6-sialyltransferase (ST6GalNAc) subfamily, designated ST6GalNAc VI, was identified by BLAST analysis of expressed sequence tags. The sequence of the cDNA clone of ST6GalNAc VI encoded a type II membrane protein with 43 amino acids composing the cytoplasmic domain, 21 amino acids composing the transmembrane region, and 269 amino acids composing the catalytic domain. The predicted amino acid sequence showed homology to the previously cloned ST6GalNAc III, IV, and V, with common amino acid sequences in sialyl motif L and S among these four enzymes. A fusion protein with protein A and extracts from L cells transfected with ST6GalNAc VI in an expression vector showed enzyme activity of alpha2,6-sialyltransferase for GM1b, GT1b, and GD1a but not toward glycoproteins. Thin layer chromatography-immunostaining revealed that the products were GD1alpha, GQ1balpha, and GT1aalpha. Northern blotting revealed that this gene was expressed in a wide range of mouse tissues such as colon, liver, heart, spleen, and brain. It is concluded that this enzyme is a novel sialyltransferase involved in the synthesis of alpha-series gangliosides in the nervous tissues and many other tissues.
The synthesis and thermolysis of imidazole quaternary salts (1977)

The pyrolysis of imidazolium halides substituted on the nitrogen atoms by alkyl or aryl groups leads to 1-substituted imidazoles. Differing substituents cleave at different rates, while the nature of the anion and the influence of substituents at C4 modify the reaction products. Tetraphenylborate and perchlorate salts fail to dealkylate. An SN2 (or SN2?) mechanism appears to be the most likely for the process.
Multibeam Optical System Incorporating a Microlens Array (1996)

Parallel recording using a multibeam laser diode array is effective for recording at a high data transfer rate. Focused spot interval equal to the track pitch is realized effectively in an optical disk drive by tilting the spots away from the track direction. However, this method is not applicable in an optical tape recording system using a rotating optical head. We studied theoretically and experimentally a new multibeam optical system incorporating a microlens array to realize a narrow spot interval. The beams that pass through the microlens can be treated as Gaussian beams. The introduction of the microlens array realized a small spot interval of 4 µm at a transmittance of 40%. This value is larger than that without the microlens array by a factor of about 20.
A new approach to the growth of langasite crystals (2002)

Preparation of the initial charge, crystal growth process and crystal quality assessment of langasite are described. The initial charge preparation is based on an original method named self-distribution high-temperature synthesis (SHS), which is applied for the first time to mixtures of lanthanum and silicon oxides and metallic gallium. Influence of the main growth conditions on the crystal quality is discussed. The advantages of using iridium as crucible material are shown. A value of the crystal size as big as 70 mm of diameter was reached. Crystal quality is evaluated to be high enough to produce monolithic 80 MHz filters.
Anomalous Hall effect in InSb layers grown by metalorganic chemical vapor deposition on GaAs substrates (1993)

InSb epitaxial layers have been grown on GaAs substrates by low-pressure metalorganic chemical vapor deposition. A 3.15-μm-thick film yielded an x-ray full width at half maximum of 171 arcsec. A Hall mobility of 76 200 cm2/V s at 240 K and a full width at half maximum of 174 arcsec have been measured for a 4.85-μm-thick epilayer. Measured Hall data have shown anomalous behavior. A decrease in Hall mobility with decreasing temperature has been observed and room-temperature Hall mobility has increased with thickness. In order to explain the anomalous Hall data, and the thickness dependence of the measured parameters, the Hall coefficient and Hall mobility have been simulated using a three-layer model including a surface layer, a bulklike layer, and an interface layer with a high density of defects. Theoretical analysis has shown that anomalous behavior can be attributed to donorlike defects caused by the large lattice mismatch and to a surface layer which dominates the transport in the material at low temperatures.