gebhart/patrepeval huggingface dataset:
Modalities and future prospects of gene therapy in heart transplantation (2009)

Heart transplantation is the treatment of choice for many patients with end-stage heart failure. Its success, however, is limited by organ shortage, side effects of immunosuppressive drugs, and chronic rejection. Gene therapy is conceptually appealing for applications in transplantation, as the donor organ is genetically manipulated ex vivo before transplantation. Localised expression of immunomodulatory genes aims to create a state of immune privilege within the graft, which could eliminate the need for systemic immunosuppression. In this review, recent advances in the development of gene therapy in heart transplantation are discussed. Studies in animal models have demonstrated that genetic modification of the donor heart with immunomodulatory genes attenuates ischaemia-reperfusion injury and rejection. Alternatively, bone marrow-derived cells genetically engineered with donor-type major histocompatibility complex (MHC) class I or II promote donor-specific hyporesponsiveness. Genetic engineering of naïve T cells or dendritic cells may induce regulatory T cells and regulatory dendritic cells. Despite encouraging results in animal models, however, clinical gene therapy trials in heart transplantation have not yet been started. The best vector and gene to be delivered remain to be identified. Pre-clinical studies in non-human primates are needed. Nonetheless, the potential of gene therapy as an adjunct therapy in transplantation is essentially intact.
Identification of an Arabidopsis Feruloyl-Coenzyme A Transferase Required for Suberin Synthesis     (2009)

All plants produce suberin, a lipophilic barrier of the cell wall that controls water and solute fluxes and restricts pathogen infection. It is often described as a heteropolymer comprised of polyaliphatic and polyaromatic domains. Major monomers include omega-hydroxy and alpha,omega-dicarboxylic fatty acids, glycerol, and ferulate. No genes have yet been identified for the aromatic suberin pathway. Here we demonstrate that Arabidopsis (Arabidopsis thaliana) gene AT5G41040, a member of the BAHD family of acyltransferases, is essential for incorporation of ferulate into suberin. In Arabidopsis plants transformed with the AT5G41040 promoter:YFP fusion, reporter expression is localized to cell layers undergoing suberization. Knockout mutants of AT5G41040 show almost complete elimination of suberin-associated ester-linked ferulate. However, the classic lamellar structure of suberin in root periderm of at5g41040 is not disrupted. The reduction in ferulate in at5g41040-knockout seeds is associated with an approximate stoichiometric decrease in aliphatic monomers containing omega-hydroxyl groups. Recombinant AT5G41040p catalyzed acyl transfer from feruloyl-coenzyme A to omega-hydroxyfatty acids and fatty alcohols, demonstrating that the gene encodes a feruloyl transferase. CYP86B1, a cytochrome P450 monooxygenase gene whose transcript levels correlate with AT5G41040 expression, was also investigated. Knockouts and overexpression confirmed CYP86B1 as an oxidase required for the biosynthesis of very-long-chain saturated alpha,omega-bifunctional aliphatic monomers in suberin. The seed suberin composition of cyp86b1 knockout was surprisingly dominated by unsubstituted fatty acids that are incapable of polymeric linkages. Together, these results challenge our current view of suberin structure by questioning both the function of ester-linked ferulate as an essential component and the existence of an extended aliphatic polyester.
GPS code and carrier multipath mitigation using a multiantenna system (2001)

Multipath is a major source of error in high precision Global Positioning System (GPS) static and kinematic differential positioning. Multipath accounts for most of the total error budget in carrier phase measurements in a spacecraft attitude determination system. It is a major concern in reference stations, such as in Local Area Augmentation Systems (LAAS), whereby corrections generated by a reference station, which are based on multipath corrupted measurements, can significantly influence the position accuracy of differential users. Code range, carrier phase, and signal-to-noise (SNR) measurements are all affected by multipath, and the effect is spatially correlated within a small area. In order to estimate and remove code and carrier phase multipath, a system comprising a cluster of five GPS receivers and antennas is used at a reference station location. The spatial correlation of the receiver data, and the known geometry among the antennas, are exploited to estimate multipath for each satellite in each antenna in the system. Generic receiver code and carrier tracking loop discriminator functions are analyzed, and relationships between receiver data, such as code range, carrier phase, and SNR measurements, are formulated and related to various multipath parameters. A Kalman filter is described which uses a combination of the available information from the antennas (receivers) in the multiantenna cluster to estimate various multipath parameters. From the multipath parameters, the code range and carrier phase multipath is estimated and compensated. The technique is first tested on simulated data in a controlled multipath environment. Results are then presented using field data and show a significant reduction in multipath error.
TIME OPERATING SYSTEM IME OPERATING SYSTEM IME OPERATING SYSTEM IME OPERATING SYSTEM (2012)

This paper investigates the sensitivity of real-tim e systems running applications under operating systems that are subject to soft-errors. A procedure for characterizing the soft error susceptibility of nod es in a logic circuit, and a heuristic procedure for select ing the set of nodes for partial duplication are described. Third, the correctness of embedded systems is currently jeopardized by soft errors that may render control systems inoperable. In general, soft errors are increasingly a problem due to smaller fabrication s izes and deployment in harsh environments. A full set of experimental results demonstrate the cost-effective tradeoffs that can be achieved. First, architects m ust understand the impact of soft errors on their desig ns. Second, they must select judiciously from among available techniques to reduce this impact in order to meet their reliability targets with minimum overhea d Two reduction heuristics, cluster sharing reduction and dominant value reduction, are used to reduce the so ft error failure rate significantly with a fraction of the overhead required for conventional TMR. However, FPGA's vulnerability to hard and soft errors is a m ajor weakness to robust configurable system design. To eliminate the soft memory errors that are induced b y cosmic rays, memory manufacturers must either produce designs that can resist cosmic ray effects or else invent mechanisms to detect and correct the errors.
Adults and children with small non-cleaved-cell lymphoma have a similar excellent outcome when treated with the same chemotherapy regimen. (1996)

Adults and children with SNCL have a similar prognosis when treated with the same chemotherapy. EFS in high-risk patients has been markedly improved by including IVAC in protocol 89-C-41, and excellent results can be achieved with only four cycles of therapy. In protocol 89-C-41, GM-CSF was not beneficial.
Electric properties of unipolar GaAs structures with ultrathin triangular barriers (1982)

Unipolar GaAs structures with built-in planar-doped triangular barriers (n-i-p+-i-n) have been investigated experimentally. Current-voltage characteristics of ultrathin (400 Å on each side) barriers have been measured at temperatures ranging from 77 to 450 K. It is found that the characteristic activation energy Ea for current of both polarities strongly depends on temperature, changing from 1.05 eV at room temperature to 0.4 eV at 77 K. Such variation of the activation energy is interpreted as a transition from the thermionic mechanism of current to thermally assisted tunneling.
MS2: Practical data privacy and security framework for data at rest in cloud (2014)

Security and performance are two major concerns in cloud, to manage balance between security and performance is really another big practical challenge for researchers today. In cloud computing, cloud consumers' or clients' data is kept on cloud service provider's premises which raises the data privacy and integrity concerns, consequently decreases degree of trust on cloud computing paradigm. In this paper, we proposed the unified data encryption architecture which ensures the data security and privacy with reasonable performance overhead of computing system. Our proposed system is practically viable and based on multilevel identity encryption approach with two level/factor identity verification process. Proposed data security architecture includes encryption and verification services both at file and block storage level to satisfy the data protection needs of different cloud service models, especially computing service (IaaS) model. In IaaS model, elastic block storage (EBS) volumes are dynamically provisioned to full-fill the additional storage requirement of running computing Virtual servers (VMs).While in storage service, data objects (files) are stored directly on shared storage media. Our solution facilitates cloud consumers to store their sensitive information and application data objects in corresponding storage devices with complete data privacy and security. It also leverages both CSP and cloud vendors for achieving transparency in security processes of cloud.
The mechanism of HAMLET-induced cell death - cellular signalling, oncogenes and clinical perspectives (2012)

Despite recent advances in cancer treatment, truly innovative approaches are required to move beyond the modest benefits achieved to date. HAMLET is a human protein-lipid complex originally discovered in breast milk able to kill a wide range of tumour cells while leaving healthy, differentiated cells unaffected. The aim of this thesis was to identify mechanisms dictating HAMLET sensitivity and to define the events that lead to cell death in response to HAMLET. In Paper I we show that the properties sensitising cells to HAMLET coincide with the ‘’Hallmarks of cancer’’. Using a combination of shRNA screens, proteomic and metabolomic technology, we identified the c-Myc oncogene and hexokinase as essential determinants of HAMLET sensitivity. HAMLET sensitivity was modified by the glycolytic state of tumor cells and HAMLET induced a metabolic paralysis in tumour cells. In paper II the initiating events for tumour cell death are elucidated. By rapid activation of a single ion channel HAMLET disturbs the flux of cations in tumour cells and subsequent cellular responses are shown to rely on this mechanism for their initiation. Ion fluxes activated p38 MAPK signalling, which was found to be crucially involved in tumour cell death. Paper III identifies oleate as the functional ligand in HAMLET formation and shows that oleate actively contributes to the induction of the cellular response to HAMLET. Both protein and lipid are needed for HAMLET’s tumoricidal effect, however. Finally, in paper IV we identify HAMLET as a new therapeutic agent in colon cancer. HAMLET caused a significant reduction in tumour numbers as well as mortality in mice carrying a human APC mutation. In tumours surviving HAMLET challenge a reduction in onco-protein expression was detected as well as an increase in expression of glycolic enzymes. By long-term prophylaxis, prevention of tumour development was achieved.
A further study on the regulation of microbial proteases. (1990)

Various agents were tested for their effects on microbial proteases, which activity was monitored by the analysis of cleaved peptide bands in SDS-polyacrylamide gel electrophoresis. Using casein as a substrate, fungal protease (type XIX) was inhibited by the phenyl methyl sulphonyl fluoride, chymostatin, antipain and leupeptin, while bacterial protease (type XXVI) was inhibited by phosphatidyl glycerol, phosphatidyl inositol and sphingosine. MS2 RNA exerted minor inhibition on the bacterial proteolysis of regulatory subunits of cyclic AMP-dependent protein kinase (A-PK). The cleavage of DNA binding protein by both proteases was inhibited, in the presence of MS2 RNA and lambda DNA. In comparison, phosphatidyl serine slightly stimulated the fungal protease on the cleavage of ribonuclease T1. RNA polymerase is a good substrate of the bacterial protease as indicated by the generation of multiple cleaved peptide fragments, whereas alkaline phosphatase is not susceptible to proteolysis.
Purinergic receptors in the brainstem mediate hypotension and bradycardia. (1988)

Adenosine acts at many sites to modulate neuronal activity. The purpose of this study was to investigate a possible role for adenosine as a neuromodulator of brainstem cardiovascular control. Microinjections of adenosine (0-2.3 nmol) were made stereotaxically into various brainstem sites. Injection of adenosine into the nucleus tractus solitarii (NTS) produced dose-related decreases in heart rate and systolic and diastolic blood pressures. Maximal changes occurred 90 seconds after injection. Injection into the area postrema also produced decreased heart rate and systolic and diastolic blood pressures. No significant effect occurred following injection into the C1 area. Adenosine 5'-triphosphate and its analogue, beta, gamma-methylene adenosine 5'-triphosphate also produced dose-related and potent vasodepressor and bradycardia effects in the NTS. Injection of 1,3-dipropyl-8-p-sulfophenylxanthine (0.92 nmol), a potent adenosine receptor antagonist, produced no effect itself, but abolished for 45 minutes the actions of further injections of adenosine and adenosine 5'-triphosphate (but not L-glutamate) in both the NTS and area postrema. Thus, NTS and area postrema injections of adenosine decrease blood pressure and heart rate in anesthetized normotensive rats through adenosine receptors located in these areas. These findings support a role for endogenous adenosine as a central modulator in cardiovascular control.
Adaptive spatial-temporal resolution optical vehicular communication system using image sensor (2017)

In this article, we introduce an image sensor–based optical vehicular communication system for performing vehicular condition resolution adaptively in the spatial domain and the temporal domain. To validate the proposed system, a dual camera–based approach is presented here, where a vision camera is used to detect multiple vehicles of interest (i.e. spatial condition), a communication link is set up, and then, a high-speed camera adapts its resolution to ensure fast processing and high-rate data communication (i.e. temporal condition). We suggest an adaptive algorithm that supports the detection of and communication multiple vehicles simultaneously in spatial-temporal condition. Moreover, we propose a combination of scale-invariant feature transform and iterative closest point for detection and classification of multiple vehicles. To prove the feasibility of our proposed scheme, we present simulation results.
Interaction between aliphatic and aromatic nitro compounds in basic media (1968)

The interaction between aromatic and aliphatic nitro compounds in basic media has been investigated. Evidence based mainly on nuclear magnetic resonance spectroscopy suggests that the brightly colored species formed are sigma complexes. The generality of the reaction is investigated and its relation to the Janovsky reaction discussed.
Genetic Parameters for Clinical Mastitis, Somatic Cell Score, Production, Udder Type Traits, and Milking Ease in First Lactation Holsteins (1999)

Genetic parameters were estimated by restricted maximum likelihood with an animal model on first lactation data of 29,284 French Holstein cows for clinical mastitis, lactation somatic cell score, milking ease, production, and nine udder type traits. The heritability was low for clinical mastitis (0.024), moderate for lactation somatic cell score (0.17) and milking ease (0.17), and ranged from 0.17 to 0.30 for type traits. A high (0.72) but lower than unity genetic correlation was found between clinical mastitis and lactation somatic cell score and indicated that both traits were genetically favorably associated. The antagonism with production was stronger for clinical mastitis than for lactation somatic cell score (genetic correlations 0.45 and 0.15, respectively). Udder depth, fore-udder attachment, and udder balance were favorably associated with lactation somatic cell score and clinical mastitis with genetic correlations ranging from -0.29 to -0.46, whereas low correlations were found with teat length. Milking ease was found to be unfavorably correlated with lactation somatic cell score (genetic correlation 0.44) but not with clinical mastitis.
Encapsulation-free hybrid organic-inorganic light-emitting diodes (2006)

The authors have fabricated encapsulation-free hybrid organic inorganic light-emitting diodes (HOILEDs), with improved air stability performance by incorporating two different metal oxide layers. One metal oxide layer is a titanium dioxide layer that provides the electron-injection layer, and the other is a molybdenum oxide that injects holes. While our HOILED device exhibits a lower threshold voltage and a higher air stability than conventional devices using a Ca∕Al cathode, a similar luminance output was observed.
E mu-bcl-2 transgene facilitates spontaneous transformation of early pre-B and immunoglobulin-secreting cells but not T cells. (1993)

To assess the lymphoid tumorigenic potential of bcl-2, mice of five independent strains expressing a bcl-2 transgene in B and/or T cells were monitored for disease up to 12 months of age. Lymphoma prevalence was minimal in the T lineage but significant, although low (3-15%), in the B lineage. The principal types of tumors were plasmacytomas secreting immunoglobulin and novel lymphomas that expressed markers such as Sca-1, CD4, Thy-1, CD34 and CD45(B220), consistent with an origin very early in B-lymphoid development. Rearrangement of the c-myc gene was common in the plasmacytomas, implying a synergistic role for myc and bcl-2 in their etiology, but was not detected in the lymphomas.
Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples after Heat Treatment (1998)

A fast, sensitive, and target contaminant-modulable method was developed to detect viable bacteria, molds, and yeasts after heat treatment. By reverse transcriptase PCR with elongation factor gene (EF-Tu or EF-1 alpha)-specific primers, the detection level was 10 cells ml of milk-1. The simplicity and rapidity (4 h) of the procedure suggests that this method may be easily transposable to other foods and other contaminants.
DNA Nanoparticles: Detection of Long-Term Transgene Activity in Brain using Bioluminescence Imaging (2011)

In this study, we used bioluminescence imaging (BLI) to track long-term transgene activity following the transfection of brain cells using a nonviral gene therapy technique. Formulations of deoxyribonucleic acid (DNA) combined with 30-mer lysine polymers (substituted with 10 kDa polyethylene glycol) form nanoparticles that transfect brain cells in vivo and produce transgene activity. Here we show that a single intracerebral injection of these DNA nanoparticles (DNPs) into the rat cortex, striatum, or substantia nigra results in long-term and persistent luciferase transgene activity over an 8- to 11-week period as evaluated by in vivo BLI analysis, and single injections of DNPs into the mouse striatum showed stable luciferase transgene activity for 1 year. Compacted DNPs produced in vivo signals 7- to 34-fold higher than DNA alone. In contrast, ex vivo BLI analysis, which is subject to less signal quenching from surrounding tissues, demonstrated a DNP to DNA alone ratio of 76- to 280-fold. Moreover, the ex vivo BLI analysis confirmed that signals originated from the targeted brain structures. In summary, BLI permits serial analysis of luciferase transgene activity at multiple brain locations following gene transfer with DNPs. Ex vivo analysis may permit more accurate determination of relative activities of gene transfer vectors.
The Effects of Available Information on Responses to School Writing Tasks (1984)

Preview this article: The Effects of Available Information on Responses to School Writing Tasks, Page 1 of 1 < Previous page | Next page > /docserver/preview/fulltext/rte/18/1/researchintheteachingofenglish15685-1.gif
Solvent deuteration enhancement of hydrophobicity: DSC study of the inverse temperature transition of elastin-based polypeptides (1991)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSolvent deuteration enhancement of hydrophobicity: DSC study of the inverse temperature transition of elastin-based polypeptidesChi Hao Luan and Dan W. UrryCite this: J. Phys. Chem. 1991, 95, 20, 7896–7900Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October 1991https://pubs.acs.org/doi/10.1021/j100173a063https://doi.org/10.1021/j100173a063research-articleACS PublicationsRequest reuse permissionsArticle Views290Altmetric-Citations45LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
A review of the anti-inflammatory properties of clindamycin in the treatment of acne vulgaris. (2010)

This article reviews anti-inflammatory properties of clindamycin, which is often used topically for the management of acne vulgaris, usually in combination with other agents. The efficacy of clindamycin in acne treatment has been shown to be sustained for more than 3 decades. It is likely that anti-inflammatory effects play an important role in the therapeutic activity of topical clindamycin.
Debris clearance by microglia: an essential link between degeneration and regeneration (2008)

Microglia are cells of myeloid origin that populate the CNS during early development and form the brain's innate immune cell type. They perform homoeostatic activity in the normal CNS, a function associated with high motility of their ramified processes and their constant phagocytic clearance of cell debris. This debris clearance role is amplified in CNS injury, where there is frank loss of tissue and recruitment of microglia to the injured area. Recent evidence suggests that this phagocytic clearance following injury is more than simply tidying up, but instead plays a fundamental role in facilitating the reorganization of neuronal circuits and triggering repair. Insufficient clearance by microglia, prevalent in several neurodegenerative diseases and declining with ageing, is associated with an inadequate regenerative response. Thus, understanding the mechanism and functional significance of microglial-mediated clearance of tissue debris following injury may open up exciting new therapeutic avenues.
Dynamics (2013)

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