gebhart/patrepeval huggingface dataset:
Interference-Free Detection of Genetic Biomarkers Using Synthetic Dipole-Facilitated Nanopore Dielectrophoresis (2016)

The motion of polarizable particles in a nonuniform electric field (i.e., dielectrophoresis) has been extensively used for concentration, separation, sorting, and transport of biological particles from cancer cells and viruses to biomolecules such as DNAs and proteins. However, current approaches to dielectrophoretic manipulation are not sensitive enough to selectively target individual molecular species. Here, we describe the application of the dielectrophoretic principle for selective detection of DNA and RNA molecules using an engineered biological nanopore. The key element of our approach is a synthetic polycationic nanocarrier that selectively binds to the target biomolecules, dramatically increasing their dielectrophoretic response to the electric field gradient generated by the nanopore. The dielectrophoretic capture of the nanocarrier–target complexes is detected as a transient blockade of the nanopore ionic current, while any nontarget nucleic acids are repelled from the nanopore by electrophoresis and thus do not interfere with the signal produced by the target’s capture. Strikingly, we show that even modestly charged nanocarriers can be used to capture DNA or RNA molecules of any length or secondary structure and simultaneously detect several molecular targets. Such selective, multiplex molecular detection technology would be highly desirable for real-time analysis of complex clinical samples.
Sequence and genetic map of <i>Meloidogyne hapla</i> : A compact nematode genome for plant parasitism (2008)

We have established Meloidogyne hapla as a tractable model plant-parasitic nematode amenable to forward and reverse genetics, and we present a complete genome sequence. At 54 Mbp, M. hapla represents not only the smallest nematode genome yet completed, but also the smallest metazoan, and defines a platform to elucidate mechanisms of parasitism by what is the largest uncontrolled group of plant pathogens worldwide. The M. hapla genome encodes significantly fewer genes than does the free-living nematode Caenorhabditis elegans (most notably through a reduction of odorant receptors and other gene families), yet it has acquired horizontally from other kingdoms numerous genes suspected to be involved in adaptations to parasitism. In some cases, amplification and tandem duplication have occurred with genes suspected of being acquired horizontally and involved in parasitism of plants. Although M. hapla and C. elegans diverged >500 million years ago, many developmental and biochemical pathways, including those for dauer formation and RNAi, are conserved. Although overall genome organization is not conserved, there are areas of microsynteny that may suggest a primary biological function in nematodes for those genes in these areas. This sequence and map represent a wealth of biological information on both the nature of nematode parasitism of plants and its evolution.
Frequency-domain analysis of biomolecular sequences (2000)

We introduce new computational and visual tools for biomolecular sequences analysis. In particular, we provide an optimization procedure improving upon traditional Fourier analysis performance in distinguishing coding from noncoding regions in DNA sequences. We also show that the phase of a properly defined Fourier transform is a powerful predictor of the reading frame of protein coding regions. Resulting color maps help in visually identifying not only the existence of protein coding areas for both DNA strands, but also the coding direction and the reading frame for each of the exons. Furthermore, we demonstrate that color spectrograms can visually provide, in the form of local 'texture', significant information about biomolecular sequences, thus facilitating understanding of local nature, structure and function.
Large-Scale System Configuration with LCFG and SmartFrog (2003)

This is the third report from the GridWeaver project. It describes how the LCFG and SmartFrog technologies have been combined, in prototype form, to create a largescale configuration system. First, we focus on the configuration aspects that must be modelled and examine how these are represented in the SmartFrog 2 language. Secondly, we discuss how the LCFG and SmartFrog systems have been combined to create a dynamic configuration deployment environment.
Thermal Conductivity Bridge for Gas Analysis (1951)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThermal Conductivity Bridge for Gas AnalysisC. C. Minter and L. M. J. BurdyCite this: Anal. Chem. 1951, 23, 1, 143–147Publication Date (Print):January 17, 1951Publication History Published online1 May 2002Published inissue 17 January 1951https://pubs.acs.org/doi/10.1021/ac60049a029https://doi.org/10.1021/ac60049a029research-articleACS PublicationsRequest reuse permissionsArticle Views53Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Adrenal aldosterone-producing adenoma: use of colonic potential in diagnosis and subtraction scanning technique for localisation. (1976)

Primary hyperaldosteronism is a potentially curable cause of hypertension, and much interest has been shown in methods of diagnosing the associated hypokalaemic hypertension and localising the adrenal adenoma. In two patients the diagnosis of primary aldosteronism was confirmed by colonic potential measurement and the adenoma localised by a new subtraction technique for early adrenal imaging applied to the use of 131I-19-iodocholesterol. Both patients underwent adrenalectomy and in each case an adenoma was removed. Blood pressure and electrolyte levels returned to normal after operation. In one patient bilateral adrenal phlebography had failed to show the tumour, and sampling of aldosterone concentrations in the adrenal veins had been unsatisfactory.
Synthesis and Characterization of Glycerol Dendrons, Self-Assembled Monolayers on Gold: A Detailed Study of Their Protein Resistance (2009)

Nonfouling surface coatings are of great interest for the development of advanced biomaterials used in biomedical and marine applications. Therefore, a lot of effort has been made to design new biocompatible materials and to understand the mechanisms of the protein repulsion. This study examines a series of polyglycerol (PG) dendrons modified by alkanethiols for their interactions with biofouling relevant proteins: fibrinogen (Fib), lysozyme (Lys), albumin (Alb), and pepsin (Pep). All polyglycerol dendrons [G1.0]-[G3.0] self-assembled monolayers with different terminal functionality (-OH, -OCH(3)) were prepared by applying simple Williamson ether formation followed by radical thiol addition to the alkene. Surface modification was performed by chemisorption of the different dendritic PG derivatives onto gold chips from ethanolic solution and then directly used in a screening with the respective proteins applying SPR spectroscopy. The effective and time-dependent SAM formation on gold was also revealed by X-ray photoelectron spectroscopy. It was demonstrated that the all polyglycerol dendrons [G1.0]-[G3.0] possess excellent resistance to the test proteins. Surprisingly, the SAMs of easily accessible [G1.0] dendron (M(w) = 426 g/mol) modified alkanethiol show the same high protein resistance as we could achieved for high molecular weight polymers (e.g., hyperbranched PG with M(n) = 2500 g/mol). However, significant changes in the amount of adsorbed proteins within the studied time frame of 24 h was not observed. Therefore, these oligoglycerol dendrons are a good alternative for the commonly used poly(ethylene glycol) (PEG).
Repairing Effects of Diglucosyl Gallic Acid on Coloring-Damaged Hair (2004)

The hair of consumers using hair colors frequently suffers from serious damage to silky feeling on their hair, of which surface turns hydrophilic with hair dye and hair bleach. The damaged hair can be dramatically recovered with applying diglucosyl gallic acid (DGA) and alkyl quaternary ammonium salt on the colored hair. These compounds form a hydrophobic layer (artificial F-layer) on the damaged surface of cuticle, and anchor so firmly that the artificial F-layer is hardly washed off with shampoo. DGA is proposed to be a new cosmetic ingredient, which is a very useful agent for the recovery of damaged hair due to coloring.
Regulation by interleukin-3 of human monocyte pro-inflammatory mediators. Similarities with granulocyte-macrophage colony-stimulating factor. (1990)

Urokinase-type plasminogen activator (u-PA), tumour necrosis factor-alpha (TNF-alpha) and interleukin-1 (IL-1) activities were measured for highly purified human monocytes cultured for 18 hr with recombinant human interleukin-3 (IL-3). IL-3 alone stimulated monocyte u-PA activity, but not TNF-alpha or IL-1 activity. However, IL-3, together with interferon-gamma (IFN-gamma), stimulated the TNF-alpha, but not IL-1, activities of monocytes from several donors. In parallel cultures, granulocyte-macrophage colony-stimulating factor (GM-CSF) behaved similarly. IL-3, like GM-CSF, synergized weakly and sometimes irregularly with lipopolysaccharide (LPS) for increased TNF-alpha and IL-1 activities. Thus, IL-3 can selectively stimulate monocyte mediator production depending on the costimulus present; however, the stimulatory properties of IL-3 vary from those of IFN-gamma and IL-4. The similarities in activity between IL-3 and GM-CSF may be explained by a common or associated IL-3/GM-CSF receptor(s), as suggested by biochemical studies.
Smart Contract-Based Access Control for the Internet of Things (2018)

This paper investigates a critical access control issue in the Internet of Things (IoT). In particular, we propose a smart contract-based framework, which consists of multiple access control contracts (ACCs), one judge contract (JC), and one register contract (RC), to achieve distributed and trustworthy access control for IoT systems. Each ACC provides one access control method for a subject-object pair, and implements both static access right validation based on predefined policies and dynamic access right validation by checking the behavior of the subject. The JC implements a misbehavior-judging method to facilitate the dynamic validation of the ACCs by receiving misbehavior reports from the ACCs, judging the misbehavior and returning the corresponding penalty. The RC registers the information of the access control and misbehavior-judging methods as well as their smart contracts, and also provides functions (e.g., register, update, and delete) to manage these methods. To demonstrate the application of the framework, we provide a case study in an IoT system with one desktop computer, one laptop and two Raspberry Pi single-board computers, where the ACCs, JC, and RC are implemented based on the Ethereum smart contract platform to achieve the access control.
Multiple wavelet threshold estimation by generalized cross validation for images with correlated noise (1999)

Denoising algorithms based on wavelet thresholding replace small wavelet coefficients by zero and keep or shrink the coefficients with absolute value above the threshold. The optimal threshold minimizes the error of the result as compared to the unknown, exact data. To estimate this optimal threshold, we use generalized cross validation. This procedure does not require an estimation for the noise energy. Originally, this method assumes uncorrelated noise. In this paper, we describe how we can extend it to images with correlated noise.
<b>Heat‐washout: a new method for measuring cutaneous blood flow rate in areas with and without arteriovenous anastomoses</b> (1996)

A new method, the heat-washout method, for measuring total cutaneous blood flow rate is introduced. The measurements were performed with a transcutaneous (tc) PO2-electrode that is capable of heating and measuring local temperature, and it is constructed with a thermostatically controlled cap. The probe was heated electrically to a selected temperature 2-10 degrees above normal skin temperature. When the temperature was stable, the heating element was turned off, and the temperature was registered every 10 s until a stable baseline temperature, Tb, was obtained. Tb was subtracted from the registered temperatures giving deltaTs that were plotted in a semilogarithmic diagram. The heat-washout was monoexponential, and the slope was used for calculating blood flow rate in accordance with the principle of Kety, using a known partition coefficient. The method was applied to the forearm in two subjects, and the results were compared to blood flow rates obtained simultaneously by the 133Xe-washout method in the same area. The equation of the regression line was y = 2.5 + 0 x 968X and the correlation coefficient was 0 x 986 at temperature levels of 37-45 degrees C. In the pulp of the thumb, blood flow rates, in arteriovenous anastomoses, were estimated in two subjects by subtracting the capillary blood flow rate, measured by 133Xe-washout, from the total cutaneous blood flow rate, measured by heat-washout. Due to a relatively low diffusions coefficient for 133Xe compared to heat, 133Xe cannot be used for measurement of blood flow rate in arteriovenous anastomoses.
Milliwatt‐class GaN‐based blue vertical‐cavity surface‐emitting lasers fabricated by epitaxial lateral overgrowth (2016)

We have achieved continuous‐wave (CW) operation of gallium nitride (GaN)‐based vertical‐cavity surface‐emitting lasers (VCSELs) fabricated by epitaxial lateral overgrowth (ELO) using dielectric distributed Bragg reflectors (DBRs) as masks for selective growth. The GaN VCSELs exhibited CW operation at a wavelength of 453.9 nm, and the maximum output power was 1.1 mW, which is the highest value reported to date. GaN‐based materials have presented challenges for obtaining DBRs with high reflectivity and a wide stopband, precise control of the cavity length and a lateral confinement structure to provide laser operation. The proposed VCSEL is immune to these concerns. Its two dielectric DBRs were obtained free from cracks. A high reflectance of more than 99.9% and a stopband with a width of 80–97 nm were obtained for both DBRs. The cavity length was controlled by epitaxial growth to as short as 4.5 µm. An ITO contact electrode on p‐type GaN, which is required for a lateral confinement structure, showed electrical reliability under a high current density of 59.6 kA cm −2 . The present data demonstrate that the fabrication process adopted here overcomes the shortcomings that have prevented the widespread use of GaN‐based VCSELs.
Nanostructured molybdenum carbides supported on carbon nanotubes as efficient catalysts for one-step hydrodeoxygenation and isomerization of vegetable oils (2011)

Nanostructured molybdenum carbides supported on multi-walled carbon nanotubes (Mo2C/CNTs) with different loadings were prepared by carbothermal hydrogen reduction method and characterized with SEM, Raman, HAADF-STEM and XRD. Raman spectra showed that the specific G-band structure of carbon nanotubes promoted the formation of molybdenum carbide at lower temperatures. Compared with noble metals, molybdenum carbide exhibited better catalytic activity and resistance to leaching. The Mo2C/CNTs catalyst also showed high activity and selectivity for one-step conversion of vegetable oils into branched diesel-like hydrocarbons, which provided a promising approach to prepare high-grade diesel fuels from renewable resources.
An alternative front-end for the AT&amp;T WATSON LV-CSR system (2011)

In previously published work, we have proposed a novel feature extraction algorithm, based on the Teager-Kaiser energy estimates, that approximates human auditory characteristics and that is more robust to sub-band noise than the mean-square estimates of standard MFCCs. We refer to the novel features as Teager energy cepstrum coefficients (TECC). Herein, we study the TECC performance under additive noise and suggest how to predict the noisy TECC deviations by estimating the subband SNR values. Then, we report on the effectiveness of the TECCs when they are used hi the acoustic front-end of the state-of-the-art AT&T WATSON large-vocabulary recognizer. The TECC front-end is tested in the real-life voice-search Speak4it application for mobile devices. It provides a 6% relative word error rate reduction w.r.t. the MFCC front-end, using the same high performance language model, lexicon and acoustic model training.
Motion segmentation and pose recognition with motion history gradients (2002)

This paper uses a simple method for representing motion in successively layered silhouettes that directly encode system time termed the timed Motion History Image (tMHI). This representation can be used to both (a) determine the current pose of the object and to (b) segment and measure the motions induced by the object in a video scene. These segmented regions are not "motion blobs", but instead motion regions naturally connected to the moving parts of the object of interest. This method may be used as a very general gesture recognition "toolbox". We use it to recognize waving and overhead clapping motions to control a music synthesis program.
Ultrametric Semantics of Reactive Programs (2011)

We describe a denotational model of higher-order functional reactive programming using ultra metric spaces and non expansive maps, which provide a natural Cartesian closed generalization of causal stream functions and guarded recursive definitions. We define a type theory corresponding to this semantics and show that it satisfies normalization. Finally, we show how to efficiently implement reactive programs written in this language using an imperatively updated data flow graph, and give a separation logic proof that this low-level implementation is correct with respect to the high-level semantics.
In vitro characterization of the mesangial phenotype in a proliferative glomerulonephritis of the rat (1997)

Our data suggest that mesangial cells from nephritic animals show a different phenotype in vitro in several subcultures compared with cells from control animals. This difference can be demonstrated in the patterns of proliferation and biosynthesis of prostaglandins and MMP-2, while immunohistological characteristics of mesangial cells were unchanged between nephritic animals and controls. This experimental in vivo-in vitro approach may serve as a model to study the mesangial phenotype in glomerular diseases.
MaxSMT-Based Type Inference for Python 3 (2018)

We present Typpete, a sound type inferencer that automatically infers Python 3 type annotations. Typpete encodes type constraints as a MaxSMT problem and uses optional constraints and specific quantifier instantiation patterns to make the constraint solving process efficient. Our experimental evaluation shows that Typpete scales to real world Python programs and outperforms state-of-the-art tools.
Multiscale Exploration of Mouse Brain Microstructures Using the Knife-Edge Scanning Microscope Brain Atlas (2011)

Connectomics is the study of the full connection matrix of the brain. Recent advances in high-throughput, high-resolution 3D microscopy methods have enabled the imaging of whole small animal brains at a sub-micrometer resolution, potentially opening the road to full-blown connectomics research. One of the first such instruments to achieve whole-brain-scale imaging at sub-micrometer resolution is the Knife-Edge Scanning Microscope (KESM). KESM whole-brain data sets now include Golgi (neuronal circuits), Nissl (soma distribution), and India ink (vascular networks). KESM data can contribute greatly to connectomics research, since they fill the gap between lower resolution, large volume imaging methods (such as diffusion MRI) and higher resolution, small volume methods (e.g., serial sectioning electron microscopy). Furthermore, KESM data are by their nature multiscale, ranging from the subcellular to the whole organ scale. Due to this, visualization alone is a huge challenge, before we even start worrying about quantitative connectivity analysis. To solve this issue, we developed a web-based neuroinformatics framework for efficient visualization and analysis of the multiscale KESM data sets. In this paper, we will first provide an overview of KESM, then discuss in detail the KESM data sets and the web-based neuroinformatics framework, which is called the KESM brain atlas (KESMBA). Finally, we will discuss the relevance of the KESMBA to connectomics research, and identify challenges and future directions.
Ultra-High Density Content Addressable Memory Based on Current Induced Domain Wall Motion in Magnetic Track (2012)

A new path to frame low power, high-density and fast integrated circuits has been rolled out by the observation of current-induced domain wall (DW) motion in magnetic track. As an advanced extension of this mechanism, high performance racetrack memory can be built up combining with magnetic tunnel junction (MTJ) read and write heads. The rapid progress of CoFeB/MgO perpendicular magnetic anisotropy (PMA) shows that the PMA MTJ can be scaled down to 20 nm while keeping fast data access. These recent discoveries allow us to design an ultra-high density content addressable memory (CAM), one of the most important applications of MRAM. The mainstream CAMs suffer from high power and large area as its conventional structure is composed of numerous large-capacity SRAM blocks in order to provide fast data access. MRAM based non-volatile CAMs have been proposed to relive the power consumption, however the density issue cannot be surmounted due to the large switching currents. In this paper, we present a design of NOR-type CAM based on DW motion in PMA magnetic tracks. The CMOS switching and sensing circuits are globally shared to optimize the cell area down to 6 F <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> /bit; the complementary dual track allows the local sensing and faster data search speed while keeping low power. By using an accuracy spice model of PMA racetrack memory and CMOS 65 nm design-kit, mixed simulations have been performed to demonstrate its functionality and evaluate its high performance.