gebhart/patrepeval huggingface dataset:
Peripheral Blood Mononuclear Phagocyte Subpopulations as Cellular Markers in Hypercholesterolemia (1996)

Mononuclear phagocytes play a major role in the development of vascular lesions in atherogenesis. The goal of our study was to characterize circulating blood monocyte subpopulations as potential cellular markers of systemic immunological abnormalities in hypercholesterolemia. In normal subjects, three-parameter immunophenotyping of whole blood revealed that 61.3 +/- 6.0% of monocytes showed "bright" expression of the lipopolysaccharide receptor (LPSR: CD14) and Fc gamma receptor I (RI: CD64) without expression of Fc gamma-RIII (CD16). Other monocyte subsets (populations 2, 3, 4, and 5) were characterized by the simultaneous expression of both Fc gamma-R's (25.6 +/- 5.0%), isolated expression of Fc gamma-RIII (9.4 +/- 1.7%), or high expression of CD33 (3.7 +/- 1.1%) with only dim expression of CD14, respectively. The smallest subset of monocytes (population 5: 2.1 +/- 0.8%) differed from the predominant population of CD14brightCD64+CD16- monocytes by additional expression of neural cell adhesion molecule (N-CAM: CD56). In a group of hypercholesterolemic patients (n = 19), high density lipoprotein cholesterol levels were negatively correlated to the population size of CD64-CD16+ monocytes. In both healthy subjects (n = 55) and hypercholesterolemic patients, the rare apolipoprotein E3/E4 and E4/E4 phenotypes were associated with a tendency toward a larger population of CD64-CD16+ monocytes. Expression of the variant activation antigen CD45RA by peripheral blood mononuclear phagocytes showed a positive correlation to plasma levels of the atherogenic lipoproteins low density lipoprotein and lipoprotein(a). These data suggest that systemic abnormalities in mononuclear phagocyte subpopulations may play a role in the pathogenesis of atherosclerosis.
Intelligent Mechatronics and Robotics (2018)

Intelligent mechatronics is a machine system that has its own entity and equips human-like or creature-like smart abilities. The concept of “intelligent” is, however, ambiguous; hence, there is no clear definition because the meanings and the nuance are modified according to individual intention of a designer who deals with the concept. Intelligent mechatronics basically consists of the electronics-mechanism unit and the information processing unit for control of the motion. The basic strategy of the adaptation is to modify the control law and algorithms according to the state of affairs around the machine. Kawato-Ito model is often utilized to build logical structures of the adaptation ability in many types of intelligent mechatronics. This model was originally proposed to explain human brain function for the voluntary motion control. The network intelligent mechatronics is often called networked robot. The intelligent abilities are created by cooperation with other factors via network.
Aqueous humor neutrophil gelatinase-associated lipocalin levels in patients with idiopathic acute anterior uveitis. (2010)

The aqueous humor NGAL level is increased in eyes with idiopathic AAU. These results imply that NGAL is associated with the regulation of inflammation in patients with AAU and could be used as a biomarker of ocular inflammation and immunomodulatory treatment response.
Kinetic Studies on Gluc-amylase (1966)

Journal Article Kinetic Studies on Gluc-amylase: III. The Influence of pH on the Rates of Hydrolysis of Maltose and Panose Get access KEITARO HIROMI, KEITARO HIROMI Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka PiefectureSakai Search for other works by this author on: Oxford Academic PubMed Google Scholar KATSUTADA TAKAHASHI, KATSUTADA TAKAHASHI Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka PiefectureSakai Search for other works by this author on: Oxford Academic PubMed Google Scholar ZEN-ICHIRO HAMAUZU, ZEN-ICHIRO HAMAUZU Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka PiefectureSakai Search for other works by this author on: Oxford Academic PubMed Google Scholar SÔZABURO ONO SÔZABURO ONO Laboratory of Biophysical Chemistry, College of Agriculture, University of Osaka PiefectureSakai Search for other works by this author on: Oxford Academic PubMed Google Scholar The Journal of Biochemistry, Volume 59, Issue 5, May 1966, Pages 469–475, https://doi.org/10.1093/oxfordjournals.jbchem.a128329 Published: 01 May 1966 Article history Received: 03 December 1965 Published: 01 May 1966
True-Breeding Targeted Gene Knock-Out in Barley Using Designer TALE-Nuclease in Haploid Cells (2014)

Transcription activator-like effector nucleases (TALENs) are customizable fusion proteins able to cleave virtually any genomic DNA sequence of choice, and thereby to generate site-directed genetic modifications in a wide range of cells and organisms. In the present study, we expressed TALENs in pollen-derived, regenerable cells to establish the generation of instantly true-breeding mutant plants. A gfp-specific TALEN pair was expressed via Agrobacterium-mediated transformation in embryogenic pollen of transgenic barley harboring a functional copy of gfp. Thanks to the haploid nature of the target cells, knock-out mutations were readily detected, and homozygous primary mutant plants obtained following genome duplication. In all, 22% of the TALEN transgenics proved knocked out with respect to gfp, and the loss of function could be ascribed to the deletions of between four and 36 nucleotides in length. The altered gfp alleles were transmitted normally through meiosis, and the knock-out phenotype was consistently shown by the offspring of two independent mutants. Thus, here we describe the efficient production of TALEN-mediated gene knock-outs in barley that are instantaneously homozygous and non-chimeric in regard to the site-directed mutations induced. This TALEN approach has broad applicability for both elucidating gene function and tailoring the phenotype of barley and other crop species.
Indication for <i>CDKN2A</i>-mutation analysis in familial pancreatic cancer families without melanomas (2012)

CDKN2A-mutation can be found in a considerable proportion of families with FPC. CDKN2A-mutation analysis should therefore be included in genetic testing in FPC families, even in the absence of reported melanomas. This strategy will enhance the recognition of individuals at risk for PC and facilitate the early detection of melanomas.
Presence of abscisic acid, a phytohormone, in the mammalian brain. (1986)

This paper reports the presence of abscisic acid, one of the most important phytohormones, in the central nervous system of pigs and rats. The identification of this hormone in brain was made after extensive purification by using a radioimmunoassay that is very specific for (+)-cis-abscisic acid. The final product of purification from mammalian brain has the same properties as authentic abscisic acid: it crossreacts in the radioimmunoassay for the phytohormone and it has the same retention properties and the same gas chromatography/mass spectrometry characteristics. Moreover, like (+)-cis-abscisic acid itself, the brain factor inhibits stomatal apertures of abaxial epidermis strips of Setcreasea purpurea Boom (Commelinaceae). The presence of abscisic acid conjugates that are present in plants has also been identified in brain.
Interactions between Viruses and Bacteria in Patients with Chronic Bronchitis (1976)

The possibility that viral infections of the respiratory tract might predispose to bacterial colonization or infection was studied in 120 patients with chronic obstructive pulmonary disease and 30 control subjects; these individuals were observed for seven years. The ratio of the number of observed to the number of expected associations between viruses and bacteria was 2.43 (P = 0.037) for the pair influenza virus and Streptococcus pneumoniae and was 2.06 (P = 0.056) for influenza virus and Haemophilus influenzae. Consistently positive, but not significant, associations were detected between rhinovirus and herpes simplex virus infections and isolations of S. pneumoniae and H. influenzae. In contrast, isolations of the nonpathogenic Haemophilus parainfluenzae could not be related to prior viral infections. Significant rises in titer of antibody to H. iniluenzae were detected on 76 occasions, and 20 (26%) of these antibody rises were associated with viral or mycoplasmal infections during the preceding 120 days. The expected number of such associations was 8.34 (ratio of number observed to number expected, 2.40; P = 0.08). These results suggest that viral infections of the respiratory tract in patients with chronic obstructive pulmonary disease are associated with increased colonization by potentially pathogenic bacteria and may also predispose to infection with H. injluenxae.
A Study on the Water Balance of the Kumgang Estuary Barrage (1997)

The major objective of this study is to evaluate the water balance of the Kumgang estuary barrage, one oof the regions with the most complex water utilization system. For the purpose of present study, two approaches are proposed which consider both the natural flow and low flow conditions, respectively. In order to make the evaluation of the low flow sequences, the flood frequency of the sequences was removed. According to the results, water deficiency is expected on the target year of 1996 in case of low inflow.
Complementary DNA Cloning and Kinetic Characterization of a Novel Intracellular Serine Proteinase Inhibitor: Mechanism of Action with Trypsin and Factor Xa as Model Proteinases (1994)

The full-length cDNA encoding a novel human intracellular serine proteinase inhibitor has been sequenced and found to encode a 376 amino acid protein (M(r) approximately 42.5K) that we designate as cytoplasmic antiproteinase. Analysis of the primary structure revealed that the cytoplasmic antiproteinase has the majority of structural motifs conserved among the greater superfamily of serine proteinase inhibitors, or serpins. On the basis of several criteria such as amino acid identity and the absence of a classical N-terminal signal peptide, the cytoplasmic antiproteinase represents a new member of the intracellular serpin family. Further inspection of the cytoplasmic antiproteinase amino acid sequence identified three potential N-glycosylation sites and Arg341-Cys342 as the reactive site P1-P1' residues, respectively. We have also employed the slow binding kinetic approach to detail the mechanism of bovine trypsin and human factor Xa inhibition by the novel cytoplasmic antiproteinase. Inhibition of trypsin by the cytoplasmic antiproteinase was preceded by a two-step mechanism corresponding to the formation of an initial loose complex, followed by an isomerization step to a more stable, tight complex. The binding of the cytoplasmic antiproteinase to trypsin occurred with a second-order association rate constant of 2.8 x 10(6) M-1 s-1 and an overall equilibrium constant of 22.5 pM, demonstrating that the factor is a potent inhibitor of this proteinase. Under the appropriate conditions, the tight complex between trypsin and the cytoplasmic inhibitor was reversible, indicated by an exponential regeneration of proteinase amidolytic activity from the preformed complex. Therefore, the tight complex appears to be stabilized predominantly by reversible bonds that form between trypsin and the cytoplasmic inhibitor. In contrast to the inhibition of trypsin, the inhibition of factor Xa amidolytic activity by the cytoplasmic antiproteinase followed a single-step binding mechanism. The apparent first-order rate constant for factor Xa inhibition was found to increase as a linear function of the inhibitor concentration range studied. Formation of the inhibitory complex between factor Xa and the cytoplasmic antiproteinase occurred with a second-order association rate constant of approximately 1.3 x 10(5) M-1 s-1 and a equilibrium constant of 3.7 nM. These findings suggests that the cytoplasmic inhibitor may initially encounter significant energy barriers for proper alignment with the substrate binding cleft of factor Xa. However, once aligned, the reaction proceeds rapidly to a tight factor Xa.inhibitor complex that dissociates at a slow rate.
Dinutuximab (2016)

Dinutuximab is the first anti-GD2 monoclonal antibody approved in combination with GM-CSF, IL-2, and RA for maintenance treatment of pediatric patients with high-risk neuroblastoma who achieve at least a partial response to first-line multiagent, multimodality therapy. Ongoing research will determine if dinutuximab could be used earlier in treatment, in nonresponders to initial therapies, in combination with chemotherapy, or in other cancers.
A single capsaicin injection partially depletes neuropeptides but does not ameliorate inflammation severity in established feline antigen induced arthritis. (1997)

These data indicate that SP and CGRP immunoreactivity is maintained in synovial and nonsynovial articular tissues of non-capsaicin treated knees during subacute joint inflammation. In the capsaicin treated AIA knees, however, there was partial neuropeptide depletion. Nerve fibers containing SP were more sensitive to the peptide depleting effects of capsaicin than were CGRP positive fibers. Depletion of SP and CGRP from some joint tissues did not correlate with a decrease in joint inflammation. Studies incorporating a broad range of dosages will be required to determine whether intraarticular treatment with capsaicin can effectively deplete neuropeptides and thereby ameliorate established inflammatory arthritis.
Synthesis of 7-Amino-6-nitrobenzodifuroxan (2006)

7-Amino-6-nitrobenzodifuroxan(ANBDF) was prepared through a four-step reaction process using 3,5-dichloroaniline as starting material.3,5-dichloro-N-chloroacetaniline(Ⅰ) was prepared in 97.7% yield by the reaction of chloro-acetic chloride with 3,5-dichloroaniline in the media of THF.Nitration of the compound(Ⅰ) with a mixture of fuming nitric acid(98%) and concentrated sulphuric acid(98%) at room temperature for 2 h gave 3,5-dichloro-2,4,6-trinitro-N-chloroacetaniline (Ⅱ) in 92.3% yield.7-Chloroacetamide-6-nitrobenzodifuroxan(Ⅲ) was obtanined in(82.5%) yield from (Ⅱ) and sodium azide in acetic acid through denitrogenization at 110 °C.The title compound(Ⅳ) was prepared by hydrolyzing of(Ⅲ) in basic media with a yield of 66%.The melting point of Ⅳ is 204～205 °C.The factors affecting the key reactions were discussed.The structures of 7-amino-6-nitrobenzodifuroxan and its three intermediates have all been determined by IR,~1H NMR,MS and elemental analysis.
G Protein Subunit Gα <sub>13</sub> Binds to Integrin α <sub>IIb</sub> β <sub>3</sub> and Mediates Integrin “Outside-In” Signaling (2010)

Integrins mediate cell adhesion to the extracellular matrix and transmit signals within the cell that stimulate cell spreading, retraction, migration, and proliferation. The mechanism of integrin outside-in signaling has been unclear. We found that the heterotrimeric guanine nucleotide-binding protein (G protein) Galpha13 directly bound to the integrin beta3 cytoplasmic domain and that Galpha13-integrin interaction was promoted by ligand binding to the integrin alphaIIbbeta3 and by guanosine triphosphate (GTP) loading of Galpha13. Interference of Galpha13 expression or a myristoylated fragment of Galpha13 that inhibited interaction of alphaIIbbeta3 with Galpha13 diminished activation of protein kinase c-Src and stimulated the small guanosine triphosphatase RhoA, consequently inhibiting cell spreading and accelerating cell retraction. We conclude that integrins are noncanonical Galpha13-coupled receptors that provide a mechanism for dynamic regulation of RhoA.
Use of a KIT-specific monoclonal antibody to bypass imatinib resistance in gastrointestinal stromal tumors (2013)

Acquired resistance to imatinib is a significant problem for the clinical management of gastrointestinal stromal tumor (GIST) patients, and second-line small molecules have shown limited efficacy in this setting. We have recently demonstrated that a monoclonal antibody targeting KIT could potentially bypass imatinib resistance in preclinical models of GIST.
Thermoelectric Materials Under Pressure (2018)

Recent advances in high‐pressure technology provide access both to novel materials and to exotic properties of known materials, thus opening exciting opportunities for fundamental as well as applied research. This review summarizes for various classes of materials the existing knowledge on the thermoelectric behavior under hydrostatic pressure to identify promising directions for future developments.
Fast Perspective Views of Images Using One-Dimensional Operations (1987)

Generating arbitrary perspective views of images portraying 3D surfaces can involve extensive computation and data I/O time because of the problems in determining visibility, and performing hidden-point removal. Appropriate 1D transforms of an image can allow hidden-point removal and perspective projection to be performed on scan lines or columns of these transforms. Perspective view generation then reduces to a series of extremely fast 1D operations. As a result, exact perspective views of 3D surfaces of unlimited size can be generated very much more rapidly than hitherto possible, making this a feasible interactive tool in image analysis. Maximum speed is possible when entire data sets can be stored in random access memory (RAM). However, the scan-line nature of the algorithm also allows sequential processing of data stored on disk, and fast image transposition methods allow the generation of views of surfaces much larger than available memory.
Electronic energy state of a periodic porous nanoscale graphite (2000)

The electronic energy states of periodic porous nanoscale graphites with acene and phenanthrene edge structures are studied with a two-dimensional, tight-binding crystal orbital method in the framework of the Hückel approximation. The electronic energy scheme of the periodic porous graphite is not only determined by the edge shape of the pore in the short-range order from the viewpoint of the calculated unit cell with an acene- or phenanthrene-edge structure, but also by the long-range order from the viewpoint of the graphite plane with an acene- or phenanthrene-edge structure. The appearance of flat bands in periodic porous nanostructured graphite have been theoretically demonstrated.
Preferential Distribution of the Fluorescent Phospholipid Probes NBD-Phosphatidylcholine and Rhodamine-Phosphatidylethanolamine in the Exofacial Leaflet of Acetylcholine Receptor-Rich Membranes from Torpedo marmorata (1995)

The distribution of the two fluorescent phospholipid analogs across acetylcholine receptor (AChR)-rich membranes from Torpedo marmorata has been studied by a combination of nonradiative fluorescence resonance energy transfer using fluorescent lipid probes and quenching of their fluorescence with Co2+ and 2,4,6-trinitrobenzenesulfonic acid. The fluorescent lipid analogs were supplied to the AChR-rich membrane or liposome suspension by simply injecting ethanol solutions of the probes into the medium. The efficiency of the fluorescence energy transfer between NBD-labeled phosphatidylcholine and rhodamine-labeled ethanolamine glycerophospholipids was measured in model membranes prepared in such a way that the probes could be targeted at the same or opposite halves of the bilayer, and the results were compared with those obtained for native AChR-rich membranes. It is shown that NBD-PC and Rho-PE can be efficiently (95%) incorporated into AChR-rich membranes and liposomes. On the basis of the comparison with model liposomes, the energy transfer experiments suggest a preferential exofacial location of the parental phospholipids in the native AChR-rich membrane. Fluorescence quenching with Co2+ and TNBS showed these two phospholipid analogs to be located predominantly in the outer leaflet of the bilayer in AChR-rich membranes. From the Co2+ quenching of the lipid analogs, it was also possible to calculate the surface potential of the outer leaflet of the membrane as being on the order of -15 mV.
Methylation of Aromatic Amines by the Wallach Method (1952)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMethylation of Aromatic Amines by the Wallach MethodWalter L. Borkowski and E. C. WagnerCite this: J. Org. Chem. 1952, 17, 8, 1128–1140Publication Date (Print):August 1, 1952Publication History Published online1 May 2002Published inissue 1 August 1952https://pubs.acs.org/doi/10.1021/jo50008a012https://doi.org/10.1021/jo50008a012research-articleACS PublicationsRequest reuse permissionsArticle Views667Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Stability of periodic nonlinear optical structures for limiting and logic (2002)

Summary form only given. Nonlinear periodic structures have been receiving increasing interest because of their potential applications. Previously proposed devices rely on optical bistability. We have proposed all-optical limiters, switches, as well a complete set of logic gates, an analog-to-digital converter, and an all-optical subtractor that rely on the nonlinear but stable response of periodic structures. We apply analytical and numerical techniques to explore the stability of multilayer structures. We investigate how the stability of input output intensity relationships depends on the material nonlinear properties.
Genotype determination of hepatitis C virus from Northern India: Identification of a new subtype (1996)

Hepatitis C virus (HCV) shows substantial nucleotide sequence diversity distributed throughout the viral genome, with many variants showing only 68-79% overall sequence homology. This has led to problems in diagnosis of HCV using commercial immunoassays. Based on clustering of homologous sequences, various genotypes and subtypes of HCV have been described from different geographical regions. In the present study, 11 isolates from India were genotyped using sequence comparison for part of the non-structural (NS5) and structural (core) regions. Parts of the genome covering 451 bp (nt 9-459) of the core gene and a 249 bp fragment (nt 7959-8207) of the NS5 gene were reverse transcribed and amplified using nested polymerase chain reaction (RT-PCR). The amplified fragments were cloned and sequenced. The classification into genotypes was done on the basis of phylogenetic analysis. Four isolates showed sequence homology to type 1b. Two of the isolates were classified as type 3a. One isolate was classified as type 3b and the remaining four isolates were found to be variants of type 3 but did not belong to any designated subtype. On the basis of phylogenetic analysis two of the unclassified isolates were put into a new subtype of 3 named as 3g. In one of these variants, parts of a 5'-noncoding (5' NCR; 204 bp), envelope-E1 (435 bp), and NS3 (502 bp) regions were also amplified, cloned, and sequenced. This study demonstrates the type 3 variants including a new subtype (3g) to be the major cause of HCV infection in India.