gebhart/patrepeval huggingface dataset:
Detection of Conformational Changes along the Kinetic Pathway of Protein Kinase A Using a Catalytic Trapping Technique (1999)

The dissociation rate constants for the two products of the reaction catalyzed by protein kinase A, ADP and phosphopeptide, were measured using a catalytic trapping technique to determine the role of product release in enzyme turnover. The enzyme was preequilibrated with ADP, and the reaction was initiated with a peptide substrate, LRRASLG, and ATP in a rapid quench flow instrument. At high, free magnesium concentrations (>2 mM), the large 'burst' in phosphopeptide production disappears, and, at low concentrations of free magnesium (0.5-1 mM), the kinetic transients become sigmoidal prior to the linear turnover phase. Increasing the concentrations of ATP or ADP did not influence the shape of the kinetic transients in the first 20 ms. ADP preequilibration protects the enzyme from inhibition by the covalent inactivator p-fluorosulfonylbenzoyl 5'-adenosine at 0.5 mM free magnesium, indicating that a competent E. ADP complex forms at low metal concentrations and the sigmoidal behavior in the catalytic trapping experiment is not due to free enzyme at high ATP concentrations. Simulations of the data indicate that ADP release is rate-limiting for turnover at high magnesium concentrations, but, at lower physiological levels of 0.5 and 1 mM, the off rate of ADP is 3- and 2-fold higher than k(cat), respectively. In contrast, the initial portions of the kinetic transients at 0.5 mM free magnesium were unaffected by phosphopeptide preequilibration, indicating that the release rate of this product is significantly larger than turnover. The transient kinetic data, coupled with a previous report [Shaffer and Adams (1999) Biochemistry 38, 5572-5581], support a phosphorylation mechanism under physiological magnesium concentrations that incorporates two partially rate-determining conformational changes, one prior to and one after the phosphoryl transfer step. We propose that the initial step activates the enzyme through key positioning of one or more active-site residues and the second step relaxes this conformation, a prerequisite for a subsequent catalytic cycle.
Progress towards a 1300 nm fibre amplifier (1998)

Optical fibre amplifiers are key devices for increasing the transmission distance, speed and capacity of optical communication systems. We report on current worldwide progress in the development of glass fibre amplifiers for amplification in the 1.3 micron transmission window. There are three candidates for a practical device for this application, all of which require a glass host which is not silica based. As a consequence, performance and application of these devices does not yet match that of the erbium doped fibre amplifier operating at 1.55 microns. However, the majority of land-based optical fibre operates at wavelengths around 1300 nm therefore there remains considerable interest for a practical amplifier. Recent trends, such as the development of wavelength-division multiplexing (WDM) systems, has meant that fibre amplifiers must meet a variety of requirements. These include high gain, high output power, a low noise figure, a broad and flat gain spectrum, high reliability, low cost, and compactness. Candidates for a 1.3 micron fibre amplifier include praseodymium doped fluoride (PDFA) or a similar device based on chalcogenide glasses, both of which have demonstrated amplification though not yet at practical levels; neodymium doped fluoride glasses for which signal gain is possible but severely limited by competing transitions; and dysprosium doped chalcogenide glasses, from which only fluorescent has been demonstrated. In this presentation we review progress in these three variations of the ubiquitous 1300 nm optical fibre amplifier
Antibodies against gonadotropin-releasing hormone (GnRH) and destruction of enteric neurons in 3 patients suffering from gastrointestinal dysfunction (2010)

Autoantibodies against GnRH can be detected independently on treatment of GnRH analogue. Whether the generation of the antibody is directly linked to neuron degeneration and chronic gastrointestinal symptoms in patients with intestinal dysmotility, remains to be answered.
Building cloud-ready video transcoding system for Content Delivery Networks (CDNs) (2012)

Video streaming traffic of both VoD (Video on Demand) and Live is exploding. Various types of businesses and many people are relying on video streaming to attract customers/users and for other purposes. Given the vast number of video stream formats (e.g., MP4, FLV) and transmission protocols (e.g., HTTP, RTMP, RTSP) for supporting varying types of playback terminals (particularly mobile devices such as iphone/ipad and Android phones), video content providers often need to transcode videos to multiple formats in order to stream to different types of users. Being time-sensitive and requiring high bandwidth, video streaming exerts high pressure on underlying delivery networks. Content Delivery Network (CDN) providers can help their customers quickly and reliably distribute stream contents to end users. In addition to distributing video streams, CDN providers typically allow their customers to perform video transcoding on CDN platforms. With the high volume of video streams and the bursty transcoding workload, CDN providers are eager to deploy elastic and optimized cloud-based transcoding platforms. In this paper, we design and build such a transcoding platform.
Fischer−Tropsch Aqueous Phase Refining by Catalytic Alcohol Dehydration (2007)

Polar products from Fischer−Tropsch synthesis, such as short-chain alcohols, carbonyl compounds, and carboxylic acids, on condensation, dissolve in the water formed in the Fischer−Tropsch process to form an aqueous product phase. Recovery of the organic compounds from this aqueous product phase involves complex separations, and catalytic dehydration of alcohols to olefins was investigated as a means to simplify the separations. Dehydration of alcohol mixtures (some containing water) was investigated to determine whether C2−C8 alcohols can be coprocessed and whether branched alcohols can be selectively dehydrated in the presence of linear alcohols. In the presence of an η-alumina catalyst, the ease of alcohol dehydration increased with increasing carbon number, and mixtures could be dehydrated completely with >95% selectivity to olefins. The molar ratio of secondary and tertiary alcohol dehydration to primary alcohol dehydration obtained with a sulfonic acid resin catalyst (Amberlyst 15) at 100−115 °C was >50:1, with a supported phosphoric acid catalyst at 170−180 °C, it was <10:1, and with an η-alumina catalyst at 275 °C, it was <2:1. A processing scheme that simplified the recovery of organic compounds from the aqueous product produced by Fischer−Tropsch synthesis is suggested.
Dielectric constant and electrical conductivity of contaminated fine-grained soils and barrier materials (1997)

Characterization of contaminated fine-grained soils and tracking of contaminant migration within barriers have been challenging because current methods and/or procedures are labor and time-intensive, and destructive. To demonstrate the effective use of both dielectric constant and electrical conductivity in the characterization of contaminated fine-grained soils, pore fluids were prepared at different ionic strengths, and were used as permeates for kaolinite, bentonite and a local soil. Then, both dielectric constant and electrical conductivity of the soils were measured by means of a capacitor over a wide range of frequencies and moisture content. It was observed that although each soil has its unique dielectric constant and electrical conductivity at a given moisture content, increases in ionic strength cause a decrease in the dielectric constant of the system at very high frequencies (MHZ), whereas the dielectric constant increases at low frequencies (kHz). Electrical conductivity of a soil-water system is independent of frequency. However, it is a function of ionic strength of the pore fluid. It is clearly demonstrated that dielectric constant and electrical conductivity of soils are functions of both moisture content and ionic strength, and can be used to characterize the spatial and temporal levels of contamination. This method/procedure can be usedmore » in estimating the level of contamination as well as the direction of contaminant movement in the subsurface without the use of extensive laboratory testing. Based on obtained results, it was concluded that the proposed method/procedure is promising because it is non-destructive and provides a quick means of assessing the spatial distribution of contaminants in fine-grained soils and barriers.« less
Bioluminescent assay of ATPase activity in embryonic material using firefly luciferase (1988)

The continuous bioluminescent assay of ATP has been adapted to the study of Mg2+-dependent ATPases, including the (Na+; K+) pump, in amphibian tissues. A discrete bioluminescent assay procedure for ATPase has also been developed. Components of the firefly luciferase assay reagent modify the observed ATPase activity but this can be circumvented by performing discrete instead of continuous measurements of enzyme activity. In assays with commercial ATPase preparations the continuous bioluminescent assay procedure gave ATPase activities 2.2-fold lower than obtained with the discrete procedure. In Xenopus oocyte or egg homogenates, in contrast, the total ATPase activity measured is stimulated eight times by the luciferase reagent, mainly through an unexplained activation of a Mg2+-independent ATPase. In other tissues, such as Xenopus brain homogenates, both the continuous and discrete monitoring procedures are equally suitable for the determination of ATPase activity.
Receptor and voltage-operated ion channels in the central nervous system. (1996)

Ion entry into neurons occurs either through receptor-operated channels (ROC) or voltage-operated channels (VOC). The function of ROC depends crucially on the action of agonists, antagonists or compounds modulating particular types of receptors (GABA A, NMDA, Ach N receptors). The function of VOC is closely connected with the activity of protein kinases and the processes of phosphorylation of membrane proteins (K+, Na+, Ca2+ channels). Gamma aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the vertebrate brain. The GABA A receptor is a oligomeric complex of multiple binding sites and chloride channel. This complex contains recognition sites for GABA, anxiolytics such as benzodiazepine, anxiogenic--beta-carboline, and convulsant such as picrotoxin. Chloride ion channel plays a crucial role in anxiogenic, anxiolytic and convulsant activities. Glutamic acid is the main endogenous neurotransmitter for N-methyl-D-aspartate (NMDA)-type excitatory amino acid receptor. NMDA receptors connected with Ca2+ channel, have multiple modulatory sites which are affected by a wide range of compounds. There are NMDA and competitive NMDA antagonists site, the glycine site, the phencyclidine (PCP) site and the binding site of Mg2+ ions in this receptor complex. Calcium entry through NMDA receptors may be important in the etiology of many psychiatric disorders. VOC mediate rapid, voltage-gated changes in ion permeability during action potentials in neurons. Electrophysiological studies indicate the existence of three types of VOC (K+, Na+, Ca2+ channels). In number of neurons various subtypes of Ca2+ channels (P, T, N and L-type) occur together. Among them, the L-type calcium channel has been first described and most thoroughly studied. The L-type calcium channel is localized on nerve terminals in the pre and postsynaptic parts, as well as on cell bodies and may be involved in the mechanism of action of psychotropic drugs. Chronic treatment with various psychotropic drugs changes the density of voltage-dependent Ca2+ channels in the central nervous system. Thus calcium entry through both VOC and ROC may be important in the etiology of many psychiatric disorders.
A Clinical Study to Evaluate the Efficacy of ELA-Max (4% Liposomal Lidocaine) as Compared With Eutectic Mixture of Local Anesthetics Cream for Pain Reduction of Venipuncture in Children (2002)

This study demonstrates that a 30-minute application of ELA-Max without occlusion is as safe and as effective for ameliorating pain associated with venipuncture as a 60-minute application of the prescription product EMLA requiring occlusion.
Model for a 15 ns 16K RAM with Josephson junctions (1979)

An experimental memory model for investigating the feasibility of a 16K RAM memory with Josephson junctions was fabricated and tested. There are nearly 4500 Josephson junctions in the design which includes array, line drivers, and address decoders. Storage element is a single flux-quantum (SFQ) cell arranged in a 2K array. Drivers and decoders are based on the principle of current steering in superconducting loops, which is a medium speed but low power approach. The measured read-access time of the model is approximately 10 ns. Power dissipation of the unselected chip is zero, whereas for a read/write cycle time of 30 ns, it amounts to about 10 /spl mu/W. Results indicate that a 16K chip is feasible electrically. The estimated access time and power dissipation are 15 ns and 40 /spl nu/W, respectively.
Substituted tetralins. 5. Analgesic properties of some diastereoisomeric N,N-dimethyl-4-phenyl-1,2,3,4-tetrahydro-2-naphthylamines (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstituted tetralins. 5. Analgesic properties of some diastereoisomeric N,N-dimethyl-4-phenyl-1,2,3,4-tetrahydro-2-naphthylaminesE. M. Kandeel and A. R. MartinCite this: J. Med. Chem. 1973, 16, 8, 947–948Publication Date (Print):August 1, 1973Publication History Published online1 May 2002Published inissue 1 August 1973https://pubs.acs.org/doi/10.1021/jm00266a020https://doi.org/10.1021/jm00266a020research-articleACS PublicationsRequest reuse permissionsArticle Views112Altmetric-Citations5LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Exfoliation of Layered Silicates through Immobilization of Imidazolium Groups (2010)

Layered octosilicate immobilized covalently with butylimidazolium groups was fully exfoliated into monolayer nanosheets in water. The thickness of the nanosheets was 1.9 nm (atomic force microscopy (AFM)). The colloidal aggregates of the nanosheets did not show X-ray diffraction (XRD) peaks at lower angles, suggesting the absence of layer stacking. After drying of the colloidal aggregates, a sharp peak at 1.9 nm was observed. This d-value agreed well with the thickness observed by AFM. The in-plane crystal structure of octosilicate was retained after exfoliation because of the presence of the XRD peak at 0.19 nm assignable to the (400) plane of octosilicate. The interlayer surface of Bim-Oct immobilized with butylimidazolium groups is suggested to be easily hydrated, which leads to the swelling and the following exfoliation into nanosheets. The significant change of the silicate surface by the immobilization is novel, which reflects the unique property of butylimidazolium groups. A transparent and colorless film was successfully obtained by spin-coating the colloidal aggregates of nanosheets on a glass substrate. An anionic dye of Orange II was intercalated into the interlayer of restacked nanosheets in the film. The immobilization of imidazolium groups on layered silicates is innovative for the preparation of nanosheets which are designable for various applications.
New perspectives for articular cartilage repair treatment through tissue engineering: A contemporary review (2014)

In this paper review we describe benefits and disadvantages of the established methods of cartilage regeneration that seem to have a better long-term effectiveness. We illustrated the anatomical aspect of the knee joint cartilage, the current state of cartilage tissue engineering, through mesenchymal stem cells and biomaterials, and in conclusion we provide a short overview on the rehabilitation after articular cartilage repair procedures. Adult articular cartilage has low capacity to repair itself, and thus even minor injuries may lead to progressive damage and osteoarthritic joint degeneration, resulting in significant pain and disability. Numerous efforts have been made to develop tissue-engineered grafts or patches to repair focal chondral and osteochondral defects, and to date several researchers aim to implement clinical application of cell-based therapies for cartilage repair. A literature review was conducted on PubMed, Scopus and Google Scholar using appropriate keywords, examining the current literature on the well-known tissue engineering methods for the treatment of knee osteoarthritis.
Structures and Functional Properties of Starch From Seeds of Three Soybean (<b><i>Glycine max</i></b> (L.) Merr.) Varieties* (2006)

Abstract Structures and functional properties of starch from high‐protein, lipoxygenase‐free and low‐linolenic acid soybean variety seeds collected 20 d prior to harvest were investigated. Soybean starches exhibit C B ‐type X‐ray diffraction patterns, and granule diameters were very small (0.7 to 4 µm). Soybeans, 20 d prior to harvest contained 10.9–11.7% starch (dry basis). Apparent amylose content was 19–22% and absolute amylose content was 11.8–16.2%. Amylopectin weight‐average molar mass ranged from 5.1 to 11.3×10 8 g/mol. Amylopectin average branch chain‐length, determined by anion‐exchange chromatography with an amyloglucosidase post‐column and pulsed amperometric detector, was very short relative to other starches (20.4–20.9). Onset gelatinization temperature ranged from 52–54°C, and Δ H was 12–13 J/g. Paste viscosity was low relative to other starches, especially peak (81–93 RVU) and final (93–106 RVU) viscosity. The apparent amylose content of the low‐linolenic acid soybean starch was significantly higher than that of high‐protein soybean starch, and absolute amylose content of low‐linolenic acid soybean starch was significantly higher than that of lipoxygenase‐free soybean starch. Based on our results, investigations on whether soybeans with different fatty acid oil composition have different starch structures would be worthwhile. Field replicates for each soybean variety exhibited high variation in starch characteristics, with further differences in starch structures and functional properties likely to be determined once variation is minimized.
Why Is Plasmodium vivax a Neglected Tropical Disease? (2011)

Plasmodium vivax malaria is a debilitating, sometimes life-threatening, and economically repressive disease of many tropical and temperate countries outside Africa, and yet it is perceived as relatively benign. The estimated cost of the global burden of vivax malaria is US$1,400,000,000-$4,000,000,000 per year [1], and more people worldwide live at risk from P. vivax than P. falciparum All age groups suffer P. vivax infections and endure repeated, incapacitating febrile attacks, severe anemia, and respiratory distress, with poor outcomes in pregnancy and learning impairment in children also apparent. Similar to other neglected diseases, P. vivax affects primarily poor people lacking access to affordable health care, trapping many in a relentless cycle of poverty because of loss of adult productivity and depletion of meager financial reserves Global malaria elimination programs are mobilized against P. falciparum, most likely because of the greater mortality rates associated with it, and draw resources away from P. vivax even though vivax malaria is harder to prevent, diagnose, and treat, and both species are co-endemic. There is a consensus among malaria experts that eliminating P. vivax will prove more technically challenging than eliminating P. falciparum [6], and that there exist fewer tools and a weaker knowledge base from which to start an effective global elimination program
Multiple scattering technique lidar (1992)

The Bernouilli-Ricatti equation is based on the single scattering description of the lidar backscatter return. In practice, especially in low visibility conditions, the effects of multiple scattering can be significant. Instead of considering these multiple scattering effects as a nuisance, we propose here to use them to help resolve the problems of having to assume a backscatter-to-extinction relation and specifying a boundary value for a position far remote from the lidar station. To this end, we have built a four-field-of-view lidar receiver to measure the multiple scattering contributions. The system has been described in a number of publications that also discuss preliminary results illustrating the multiple scattering effects for various environmental conditions. Reported here are recent advances made in the development of a method of inverting the multiple scattering data for the determination of the aerosol scattering coefficient.
[The effect of transforming growth factor-beta on collagen expression by human embryonic fibroblasts]. (1995)

Transforming growth factor-beta (TGF-beta) is a cytokine with diverse biological activity. It can regulate the metabolic function of extracellular matrix (ECM) and play an important role in the development of pulmonary fibrosis. Collagen are major ECM components that are responsible for normal lung structure and function. Collagen accumulation is a major feature of pulmonary fibrosis. In order to study the mechanism of pulmonary fibrosis, we examined the effects of TGF-beta on the production, mRNA expression of type I collagen and type IV collagen by cultures of human embryonic lung fibroblasts. The results indicated that TGF-beta can induce collagen formation and type I collagen, type IV collagen mRNA expression without affecting cell proliferation. We concluded that TGF-beta plays a role in collagen accumulation of pulmonary fibrosis and the changes in collagen production appear to be mediated on collagen mRNA level.
Broadband 60-W HF linear amplifier (1971)

The design, construction and performance of a solid-state untuned 2-30 MHz amplifier is described. Operation is from 28 V dc. Overall CW efficiency is greater than 47 percent at 60-w unfiltered output. Amplifier efficiency for two-tone 60-W PEP output is greater than 31 percent. two-tone linearity over the frequency range is -30 dB or better at output powers from 5-60 W. Broadband transmission-line transformers wound on ferrite toroids are used throughout for impedance matching. Hybrids of similar construction are used for output-power adding.
The flash memory read path: building blocks and critical aspects (2003)

The ever-increasing demand for portable equipment is leading to the use of flash memory devices for nonvolatile storage. Fast access time and low power consumption are obviously key requirements. Moreover, multilevel storage techniques are mandatory to reduce the cost per megabit. As a consequence, the READ operation becomes more and more critical, and optimized solutions are needed for almost all circuits involved in the read path. This paper examines the whole read path from addresses to data output, and describes several schemes developed to achieve the required high performances.