gebhart/patrepeval huggingface dataset:
Variations in the relative proportions of microheterogeneous forms of plasma glycoproteins in pregnancy and disease. (1982)

Using lectin affinity crossed immunoelectrophoresis with concanavalin A in the first dimension and electroendosmotic elution with sugar in the second dimension, the microheterogeneity of a range of plasma proteins was examined. Of the five chosen proteins, alpha 1-protease inhibitor and caeruloplasmin displayed complex patterns, with more than four components. Alpha 1-Antichymotrypsin was composed of three or four components whilst alpha 1-acid glycoprotein and alpha 2-HS glycoprotein displayed two, three or four components. The number of components seen in these proteins depended on the serum sample origin. In pregnancy and in patients receiving exogenous aestrogen the relative proportions of the components of all five proteins were altered in the direction of less con A binding; however alpha 1-acid glycoprotein and alpha 1-antichymotrypsin showed the greater change. In acute disorders the proportions of protein components of alpha 1-antichymotrypsin and alpha 1-acid glycoprotein were altered towards a higher level of con A binding components. There is no significant alteration in con A binding associated with the chronic inflammatory response to cancer and rheumatoid arthritis. There was a general reduction of con A binding in all five plasma proteins in conditions when there was a high blood aestrogen level. This decreased affinity for con A was independent of the overall effect of the aestrogen on the serum concentration of the plasma protein. These results suggest that the glycosylation of plasma proteins is probably under the same regulatory system.
Purification and properties of squirrel monkey (Saimiri sciureus) corticosteroid binding globulin (1988)

Corticosteroid binding globulin (CBG), a serum glycoprotein which binds glucocorticoids and progestins with high affinity, is widely distributed throughout the animal world. Although its charge and size characteristics have largely been conserved across species, we found the behavior of CBG in squirrel monkey (Saimiri sciureus) serum during fractionation by polyacrylamide gel electrophoresis or Sephadex chromatography was consistent with a molecule about twice the size of that found in most species. To more fully understand the basis for this difference, we purified the protein by sequential affinity and DEAE-Sepharose chromatographies. The final product was obtained in greater than 60% yield and was found to migrate as a single homogeneous band when examined by electrophoresis at pH 8.3 in polyacrylamide gels varying total acrylamide concentration or under conditions of severe protein overload. The steroid binding specificity of the purified protein was identical with that of the protein in the starting serum. The ultraviolet absorption spectrum of the isolated CBG-steroid complexes revealed that the protein had no pyridine nucleotide cofactor or nucleic acid. Amino acid analyses showed that the composition of the squirrel monkey protein is quite similar to that of CBG molecules from other species but distinct from albumins, hemoglobin, or rabbit progesterone receptor. In contrast to the single protein band observed following electrophoresis under normal conditions, separations in the presence of sodium dodecyl sulfate (SDS) resolved the pure protein into two bands: one at 54,000 daltons and one at 57,000 daltons.(ABSTRACT TRUNCATED AT 250 WORDS)
A method of redundant clocking detection and power reduction at RT level design (1997)

Article Free Access Share on A method of redundant clocking detection and power reduction at RT level design Authors: Mitsuhisa Ohnishi Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, Japan Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, JapanView Profile , Akihisa Yamada Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, Japan Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, JapanView Profile , Hiroaki Noda Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, Japan Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, JapanView Profile , Takashi Kambe Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, Japan Precision Technology Development Center, Sharp Corporation, 2613-1, Ichinomoto, Tenri, Nara 632, JapanView Profile Authors Info & Claims ISLPED '97: Proceedings of the 1997 international symposium on Low power electronics and designAugust 1997 Pages 131–136https://doi.org/10.1145/263272.263307Published:01 August 1997Publication History 23citation177DownloadsMetricsTotal Citations23Total Downloads177Last 12 Months6Last 6 weeks2 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
A microchannel plate intensified, subnanosecond, X-ray imaging camera (1989)

We have developed a microchannel plate intensified, subnanosecond X-ray detector for X-ray imaging experiments. It consists of an X-ray photocathode, a microchannel plate, electrostatic focusing optics and a subnanosecond phosphor. The detector is used for one dimensional imaging and spectroscopic measurements. Signals are recorded using either a one dimensional Reticon camera or a streak camera. The microchannel plates employ an X-ray photocathode (CuI or CsI) deposited on the front surface of the microchannel plate to enhance their soft X-ray efficiency. Electrostatic focusing of the electrons exciting the microchannel plate also enhances the gain of the detector by an order of magnitude. The electrons are accelerated to 20 kV before striking a fast phosphor (indium doped cadmium sulfide). The detector output is coupled to a Reticon camera or a streak camera with a fiber optic array. We have built and calibrated more than twenty microchannel plate intensified detectors. Efficiencies in excess of 1000 (w/cm2 output per w/cm2 input) have been demonstrated. The time response of the detector is less than 500 ps. The efficiency if the CuI and CsI X-ray photocathodes have been measured from 450 eV to 1300 eV at the Stanford Synchrotron Radiation Laboratory. Data are presented on the efficiency, time response, spectral response and the spatial resolution of the detectors.
A Novel Dual-Lidar Calibration Algorithm Using Planar Surfaces (2019)

Multiple lidars are prevalently used on mobile vehicles for rendering a broad view to enhance the performance of localization and perception systems. However, precise calibration of multiple lidars is challenging since the feature correspondences in scan points cannot always provide enough constraints. To address this problem, the existing methods require fixed calibration targets in scenes or rely exclusively on additional sensors. In this paper, we present a novel method that enables automatic lidar calibration without these restrictions. Three linearly independent planar surfaces appearing in surroundings is utilized to find correspondences. Two components are developed to ensure the extrinsic parameters to be found: a closed-form solver for initialization and an optimizer for refinement by minimizing a nonlinear cost function. Simulation and experimental results demonstrate the high accuracy of our calibration approach with the rotation and translation errors smaller than 0.05rad and 0.1m respectively.
Modal Logic and its Applications. (1972)

Journal Article Book Reviews Get access Modal Logic and its Applications. By D. Paul Snyder. (New York: Van Nostrand Reinhold. 1971. Pp. xiv + 335. Price £6.25) M. J. Cresswell M. J. Cresswell Search for other works by this author on: Oxford Academic Google Scholar The Philosophical Quarterly, Volume 22, Issue 89, October 1972, Pages 369–370, https://doi.org/10.2307/2218320 Published: 01 October 1972
Interfacial roughness effects on interlayer coupling in spin valves grown on different seed layers (2000)

We have studied the behavior of interlayer coupling in giant magnetoresistance spin valves as a function of seed layer composition and spacer layer thickness. Using in situ scanning tunneling microscopy, we have measured directly the roughness of the top surface of the lower ferromagnetic layer. We find that the seed layer composition is correlated to the roughnesses of the interfaces inside the spin valve. Interlayer coupling increases with decreasing Cu spacer layer thickness and with increasing interfacial roughness. Results favorably compare to a topographically derived magnetostatic interaction as described by a modified version of Néel’s “orange peel” model.
Role of O-Acetylation in the Immunogenicity of Bacterial Polysaccharide Vaccines (2018)

The incidence of infectious diseases caused by several bacterial pathogens such as <i>Haemophilus influenzae</i> type b, <i>Streptococcus pneumoniae</i>, and <i>Neisseria meningitidis</i>, has been dramatically reduced over the last 25 years through the use of glycoconjugate vaccines. The structures of the bacterial capsular polysaccharide (CPS) antigens, extracted and purified from microbial cultures and obtained with very high purity, show that many of them are decorated by O-acetyl groups. While these groups are often considered important for the structural identity of the polysaccharides, they play a major role in the functional immune response to some vaccines such as meningococcal serogroup A and <i>Salmonella typhi</i> Vi, but do not seem to be important for many others, such as meningococcal serogroups C, W, Y, and type III Group B <i>Streptococcus</i>. This review discusses the O-acetylation status of CPSs and its role in the immunological responses of these antigens.
Dietary supplementation with very long‐chain n‐3 fatty acids in man decreases expression of the interleukin‐2 receptor (CD25) on mitogen‐stimulated lymphocytes from patients with inflammatory skin diseases (1994)

T-cell activation and cytokine production play an important role in several chronic inflammatory diseases. Because n-3 fatty acids exert beneficial effects on the clinical state of some of these diseases, we examined the effect of dietary supplementation of n-3 fatty acids on T-cell proliferation, expression of CD25 (interleukin-2 receptor alpha-chain), secretion of interleukin-2, interleukin-6 and tumour necrosis factor from T-cells from patients with psoriasis and atopic dermatitis. During 4 months, 21 patients supplied 6 g of highly concentrated ethyl esters of EPA and DHA in gelatin capsules daily to their diet. In the control group 20 patients supplied 6 g per day of corn oil in gelatin capsules to their diet. Eicosapentaenoic acid (20:5, n-3) of serum phospholipids increased from 14 (min 4-max 42) to 81 (min 59-max 144) mg l-1 (P < 0.01) in patients with atopic dermatitis receiving n-3 fatty acids, and from 25 (min 7-max 66) to 74 (min 46-max 142) mg l-1 (P < 0.01) in patients with psoriasis, whereas docosahexaenoic acid (22:6, n-3) increased from 65 (min 46-max 120) to 92 (min 54-max 121) mg l-1 (P < 0.05) and from 81 (min 38-max 122) to 92 (min 63-max 169) mg l-1 (NS) in atopic and psoriatic patients, respectively. The changes in the serum phospholipid fatty acid profile in the groups receiving n-3 fatty acids, correlate to the dietary intake of corresponding fatty acids.(ABSTRACT TRUNCATED AT 250 WORDS)
On the Lubrication of Crosshead-pin Bearing with Eccentric Journal (1982)

Usually the lubrication of conventional type crosshead-pin bearings in 2-stroke cycle diesel engines is in quite severe condition due to an oscillating movement of the bearings where the load is always in a downward direction, i.e. on the bottom half bearings. This paper refers to an analysis on the lubrication performance of a special type crosshead-pin bearing equipped with eccentric journals which is expected to have the oil-pumping ability. From the analyses of both the journal motion and the oil-film pressure distribution, it is cleared that the oil-feeding action to each sliding surface from a circumferential oil groove is promoted by the alternate unloading of each bearing. The results of the analyses also show that, for the optimum performance without loss of the above effect, the distance between journal centers should be kept at from 0.75 to 1.25 times the radial clearance, and that the width ratio of the main journal to the whole bearing should be kept at from 0.7 to 0.8.
Investigation of minimum inventory variability scheduling policies in a large semiconductor manufacturing facility (1997)

This paper describes some problems and investigations encountered when implementing new resource scheduling policies in a large semiconductor manufacturing facility (FAB). The FAB described here uses a product release policy based on customer orders and a work-in-progress (WIP) chart. The scheduling of resource tools is done on a first in, first out (FIFO) basis on high speed tools and due date first (DDF) at bottleneck tools, except for high priority LOTS, called MAXIs. This presentation describes briefly the theory behind minimum inventory variability scheduling policies. A heuristic explanation of the minimum inventory variability for resource scheduling policies is given here. Finally a large semiconductor manufacturing facility is discussed in generic terms, including (sanitized) data collection. The results of the baseline output and historical data are compared to MIVSP.
Chemical composition of atmospheric nanoparticles during nucleation events in Atlanta (2005)

We report the first direct, in situ measurements of the chemical composition of size‐segregated atmospheric nanoparticles in the 6–15 nm diameter range. These measurements were made of ambient aerosol directly following nucleation events in Atlanta, Georgia, during the 2002 Aerosol Nucleation and Real‐time Characterization Experiment (ANARChE). The recently developed Thermal Desorption Chemical Ionization Mass Spectrometer (TDCIMS) was used to make these measurements and featured a new inlet that delivers mass of charged and size‐segregated nanoparticles at sufficiently high rates to enable analysis at a typical time resolution of 10 min. Measurements in both the positive and negative ion spectra revealed that particles formed recently from nucleation events have enhanced concentrations of ammonium and sulfate and that to within the uncertainty of our measurements, ammonium sulfate could account for all of the sampled nanoparticle mass. No other compounds were detected in the particles during these events. Concurrent measurements of particle hygroscopicity and volatility, made using a Nanometer Tandem Differential Mobility Analyzer, support the conclusion that ammonium and sulfate are primary components of these newly formed particles.
Disease-related phenotypes in a Drosophila model of hereditary spastic paraplegia are ameliorated by treatment with vinblastine (2005)

Hereditary spastic paraplegias (HSPs) are a group of neurodegenerative diseases characterized by progressive weakness and spasticity of the lower limbs. Dominant mutations in the human SPG4 gene, encoding spastin, are responsible for the most frequent form of HSP. Spastin is an ATPase that binds microtubules and localizes to the spindle pole and distal axon in mammalian cell lines. Furthermore, its Drosophila homolog, Drosophila spastin (Dspastin), has been recently shown to regulate microtubule stability and synaptic function at the Drosophila larval neuromuscular junction. Here we report the generation of a spastin-linked HSP animal model and show that in Drosophila, neural knockdown of Dspastin and, conversely, neural overexpression of Dspastin containing a conserved pathogenic mutation both recapitulate some phenotypic aspects of the human disease, including adult onset, locomotor impairment, and neurodegeneration. At the subcellular level, neuronal expression of both Dspastin RNA interference and mutant Dspastin cause an excessive stabilization of microtubules in the neuromuscular junction synapse. In addition, we provide evidence that administration of the microtubule targeting drug vinblastine significantly attenuates these phenotypes in vivo. Our findings demonstrate that loss of spastin function elicits HSP-like phenotypes in Drosophila, provide novel insights into the molecular mechanism of spastin mutations, and raise the possibility that therapy with Vinca alkaloids may be efficacious in spastin-associated HSP and other disorders related to microtubule dysfunction.
Spatial resolution enhancement of a Brillouin-distributed sensor using a novel signal processing method (1999)

It is known that the ultimate spatial resolution for a Brillouin-based sensor is limited by the lifetime of the phonons in the fiber that mediate the Brillouin loss process. At optical pulse widths less than 10 ns (corresponding to one meter spatial resolution) the Brillouin line width is considerably broadened, causing a severe penalty in resolving the Brillouin frequency shift. Around 5 ns the Brillouin line width is too broad to allow an accurate frequency determination. The fiber optics group at the University of New Brunswick, Canada, has recently developed an automated system for strain measurements in a distributed sensing system that uses a novel signal processing technique to measure strain at resolutions finer than the Brillouin line width limit. Strain has been resolved to 20 /spl mu//spl epsiv/ at 500 mm and to 40 /spl mu//spl epsiv/ at 250 mm.
In vivo or in vitro anti-CD3 epsilon chain monoclonal antibody therapy for the prevention of lethal murine graft-versus-host disease across the major histocompatibility barrier in mice. (1994)

We explored the use of a single in vivo dose of an anti-CD3 epsilon mAb for graft-vs-host disease (GVHD) prevention in mice. Anti-CD3 epsilon (145-2C11) given on the day of bone marrow (BM) transplant was highly effective in preventing GVHD in a dose-dependent manner. However, mice experienced acute toxicity as measured by early post-BM transplantation mortality, severe weight loss, and cachexia, which is likely due to T cell-dependent lymphokine production. Donor T cells were partly responsible because toxicity still occurred in mice injected with an allelic specific anti-CD3 epsilon mAb that only reacted with the donor strain. Toxicity was also dependent on the number of radioresistant host T cells present at the time of transplant, because toxicity still occurred when recipients were depleted of mature T cells in vivo with anti-CD4+ anti-CD8 mAbs, and were given T cell-depleted BM from SCID mice. Toxicity was eliminated by treating BM with anti-CD3 epsilon mAb in vitro before BM transplant instead of administering the mAb in vivo. However, GVHD protection was not complete. Direct comparison of the in vivo and in vitro approaches revealed that the in vivo administration of anti-CD3 epsilon mAb was more effective for GVHD protection and that a combination of the approaches was not beneficial. Flow cytometry studies were performed to assess the degree of CD3 epsilon chain modulation and T cell depletion obtained with in vitro vs in vivo anti-CD3 epsilon chain mAb. In mice given syngeneic BM transplants to avoid the confounding effects of GVHD, we found that in vivo anti-CD3 epsilon mAb administration induced a more prolonged period of CD3 epsilon chain modulation and/or T cell depletion than either the in vitro approach or a third approach that involved anti-CD3 epsilon F(ab')2 fragments administered in vivo. These data indicate that targeting the Ag-specific TCR, especially in such a way as to avoid toxicity associated with T cell activation, may provide an important key for developing future GVHD therapies.
Surface Roughness of Stainless Steel Bender Mirrors for Focusing Soft X-rays (2005)

We have used polished stainless steel as a mirror substrate to provide focusing of soft x-rays in grazing incidence reflection. The substrate is bent to an elliptical shape with large curvature and high stresses in the substrate require a strong elastic material. Conventional material choices of silicon or of glass will not withstand the stress required. The use of steel allows the substrates to be polished and installed flat, using screws in tapped holes. The ultra-high-vacuum bender mechanism is motorized and computer controlled. These mirrors are used to deliver focused beams of soft x-rays onto the surface of a sample for experiments at the Advanced Light Source (ALS). They provide an illumination field that can be as small as the mirror demagnification allows, for localized study, and can be enlarged, under computer control, for survey measurements over areas of the surface up to several millimeters. The critical issue of the quality of the steel surface, polished and coated with gold, which limits the minimum achievable focused spot size is discussed in detail. Comparison is made to a polished, gold coated, electroless nickel surface, which provides a smoother finish. Surface measurements are presented as power spectral densities, as a function of spatial frequency. The surface height distributions measured with an interferometric microscope, and complemented by atomic force microscope measurements, are used to compute power spectral densities and then to evaluate the surface roughness. The effects of roughness in reducing the specular reflectivity are verified by soft x-ray measurements.
Identification of intramolecular interactions in adrenergic receptors. (1992)

Adrenergic receptors are representative of a large family of plasma membrane receptors that interact with G proteins during the process of transmembrane signal transduction. G protein-coupled receptors have a primary structure that is homologous to bacteriorhodopsin and are proposed to have a similar three-dimensional structure; however, it has not yet been possible to examine this hypothesis experimentally. We have used a novel mutagenesis approach to identify intramolecular interactions. Our results indicate that specific amino acids in the seventh hydrophobic segment of alpha 2 and beta 2 adrenergic receptors lie adjacent to the first hydrophobic segment. These studies provide the first experimental evidence defining spatial relationships that exist in the three-dimensional structure of adrenergic receptors.