gebhart/patrepeval huggingface dataset:
Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors (2007)

Previous compositional studies of pre-mRNA processing complexes have been performed in vitro on synthetic pre-mRNAs containing a single intron. To provide a more comprehensive list of polypeptides associated with the pre-mRNA splicing apparatus, we have determined the composition of the bulk pre-mRNA processing machinery in living cells. We purified endogenous nuclear pre-mRNA processing complexes from human and chicken cells comprising the massive (>200S) supraspliceosomes (a.k.a. polyspliceosomes). As expected, RNA components include a heterogeneous mixture of pre-mRNAs and the five spliceosomal snRNAs. In addition to known pre-mRNA splicing factors, 5' end binding factors, 3' end processing factors, mRNA export factors, hnRNPs and other RNA binding proteins, the protein components identified by mass spectrometry include RNA adenosine deaminases and several novel factors. Intriguingly, our purified supraspliceosomes also contain a number of structural proteins, nucleoporins, chromatin remodeling factors and several novel proteins that were absent from splicing complexes assembled in vitro. These in vivo analyses bring the total number of factors associated with pre-mRNA to well over 300, and represent the most comprehensive analysis of the pre-mRNA processing machinery to date.
<sup>1</sup>H NMR Investigation of Solvent Effects in Aromatic Stacking Interactions (2001)

One of the marquis challenges in modern Organic Chemistry concerns the design and synthesis of abiotic compounds that emulate the exquisite complex structures and/or functions of biological macromolecules. Oligomers possessing the propensity to adopt well-defined compact conformations, or foldamers, have been attained utilizing hydrogen bonding, torsional restriction, and solvophobic interactions.(1) In this laboratory, aromatic electron donor--acceptor interactions have been exploited in the design of aedamers--foldamers that adopt a novel, pleated secondary structure in aqueous solution. Herein is reported detailed (1)H NMR binding studies of aedamer monomers that were carried out in solvents and solvent mixtures covering a broad polarity range. Curve-fitting analysis of the binding data using a model that incorporated the formation of higher order and self-associated complexes yielded a linear free energy relationship between the free energy of complexation and the empirical solvent polarity parameter, E(T)(30). From these studies, the association of electron-rich and electron-deficient aedamer monomers was seen to be driven primarily by hydrophobic interactions in polar solvents. However, the magnitude of these interactions is modulated to a significant extent by the geometry of the donor--acceptor complex, which, in turn, is dictated by the electrostatic complementarity between the electron-deficient and electron-rich aromatic faces of the monomers.
my2cents &#x2014; Digitizing consumer opinions and comments about retail products (2010)

Consumers seek for trusted advice during buying decision. Brand owners and retailers invest large sums in marketing and market research, trying hard to find out what customers really want. my2cents is a mobile application for reading and sharing comments and ratings on retail products. Consumers have access to comments about a product via their mobile phone and can share their own product experience with other consumers and within their social networks. They benefit from the experience of other consumers and trusted friends. Brand owners and retailers benefit from tracking consumer opinions and brand recognition at low cost. This paper describes the development of this application and first results from deployment in practice.
Adaptive reduction of heart sounds from lung sounds using fourth-order statistics (1997)

When recording lung sounds, an incessant noise source occurs due to heart sounds. This noise source severely contaminates the breath sound signal and interferes in the analysis of lung sounds. In this paper, an adaptive heart-noise reduction method, based on fourth-order statistics (FOS) of the recorded signal, without requiring recorded "noise-only" reference signal, is presented. This algorithm uses adaptive filtering to preserve the entire spectrum. Furthermore, the proposed filter is independent of Gaussian uncorrelated noise and insensitive to the step-size parameter. It converges fast with small excess errors and, due to the narrow-band nature of heart noise (HN), it requires a very small number of taps. Results from experiments with healthy subjects indicate a local HN reduction equal to or greater than 90%.
Polymeric Gas Separation Membranes (2012)

This short Perspective conveys to the general reader of Macromolecules basic approaches of materials science of polymeric membranes for gas and vapor separation. The relations between the polymer structure and transport properties of rubbery and glassy membrane materials are considered. On the basis of acquired information, several methods for quantitative prediction of permeability were developed, and their comparative analysis is given in the Perspective. The past decade was marked by the appearance of a number of novel interesting membrane materials, which will be briefly described in the text. In conclusion, novel approaches for achieving highly permeable and permselective materials (e.g., mixed matrix membranes) will be considered as well as several relevant but not solved so far problems of membrane gas separation.
The mechanism of sperm–oocyte fusion in mammals (2004)

Sperm-oocyte fusion is one of the most impressive events in sexual reproduction, and the elucidation of its molecular mechanism has fascinated researchers for a long time. Because of the limitation of materials and difficulties in analyzing membrane protein-protein interactions, many attempts have failed to reach this goal. Recent studies involving gene targeting have clearly demonstrated the various molecules that are involved in sperm-oocyte binding and fusion. Sperm ADAMs (family of proteins with a disintegrin and metalloprotease domain), including fertilin alpha, fertilin beta and cyritestin, have been investigated and found to be important for binding rather than for fusion and painstaking studies have raised suspicions that their putative receptors, oocyte integrins, are necessary for the sperm-oocyte interaction. Recently, several studies have focused the spotlight on CD9 and glycosylphosphatidylinositol (GPI)-anchored proteins on oocytes, and epididymal protein DE on sperm, as candidate molecules involved in sperm-oocyte fusion. Lack of, or interference with the function of, these proteins can disrupt the sperm-oocyte fusion without changing the binding. In this review we highlight the candidate molecules involved in the sperm-oocyte interaction suggested from the recent progress in this research field.
A new charge-trapping technique to extract SILC-trap time constants in SiO/sub 2/ (2006)

A new experimental technique for investigating the trapping-detrapping time constants for electrons at stress-induced defects in the silicon oxide is presented. The new technique is based on the gate-stress measurement for flash memories, with the application of a pulsed gate voltage. Data for 512 Kbit NOR-flash arrays are presented, and analyzed by analytical and Monte Carlo models for the trap-assisted tunneling mechanism under pulsed conditions. Comparison between experimental data and calculations for selected cells allows for an estimation of the energy and spatial depth of the oxide traps
Natural Symbols: Explorations in Cosmology. (1972)

Book Reviews Get access Natural Symbols : Explorations in Cosmology. By Mary Douglas. (London : Barrie & Bockliff/The Cresset Press. 1970. Pp. xvii + 177. Price £2.25) David R. Bell David R. Bell Search for other works by this author on: Oxford Academic Google Scholar The Philosophical Quarterly, Volume 22, Issue 88, July 1972, Pages 280–282, https://doi.org/10.2307/2218248 Published: 01 July 1972
The Citicoline Brain Injury Treatment (COBRIT) Trial: Design and Methods (2009)

Traumatic brain injury (TBI) is a major cause of death and disability. In the United States alone approximately 1.4 million sustain a TBI each year, of which 50,000 people die, and over 200,000 are hospitalized. Despite numerous prior clinical trials no standard pharmacotherapy for the treatment of TBI has been established. Citicoline, a naturally occurring endogenous compound, offers the potential of neuroprotection, neurorecovery, and neurofacilitation to enhance recovery after TBI. Citicoline has a favorable side-effect profile in humans and several meta-analyses suggest a benefit of citicoline treatment in stroke and dementia. COBRIT is a randomized, double-blind, placebo-controlled, multi-center trial of the effects of 90 days of citicoline on functional outcome in patients with complicated mild, moderate, and severe TBI. In all, 1292 patients will be recruited over an estimated 32 months from eight clinical sites with random assignment to citicoline (1000 mg twice a day) or placebo (twice a day), administered enterally or orally. Functional outcomes are assessed at 30, 90, and 180 days after the day of randomization. The primary outcome consists of a set of measures that will be analyzed as a composite measure using a global test procedure at 90 days. The measures comprise the following core battery: the California Verbal Learning Test II; the Controlled Oral Word Association Test; Digit Span; Extended Glasgow Outcome Scale; the Processing Speed Index; Stroop Test part 1 and Stroop Test part 2; and Trail Making Test parts A and B. Secondary outcomes include survival, toxicity, and rate of recovery.
Toward a framework for preparing and executing adaptive grid programs (2002)

This paper describes the program execution framework being developed by the Grid Application Development Software (GrADS) Project. The goal of this framework is to provide good resource allocation for Grid applications and to support adaptive reallocation if performance degrades because of changes in the availability of Grid resources. At the heart of this strategy is the notion of a configurable object program, which contains, in addition to application code, strategies for mapping the application to different collections of resources and a resource selection model that provides an estimate of the performance of the application on a specific collection of Grid resources. This model must be accurate enough to distinguish collections of resources that will deliver good performance from those that will not. The GrADS execution framework also provides a contract monitoring mechanism for interrupting and remapping an application execution when performance falls below acceptable levels.
Ka-band compact integrated high power amplifiers for VSAT satellite communication ground terminal (2000)

This paper describes the design and performance of new compact and low cost Ka-band power amplifiers. The main features of the power amplifiers are: 27 dBm to 35 dBm minimum output power for -16 dBm input power over the frequency range of 27.5-31 GHz. To reduce losses MIC, MMIC and waveguide technologies are employed in the development and fabrication of this set of power amplifiers. Employing waveguide technology has minimized losses in the power combiner. Three power amplifiers are described in this paper. The first amplifier is a 0.5 W power amplifier, the second is a 1.5 W power amplifier, and the third amplifier is a 3.2 W power amplifier.
한약재 유해물질 모니터링 사업 (1) - 유통 한약재의 중금속에 관한 연구 - (2002)

Objectives : This study was performed to determine the harmful heavy metal ions(Pb, Hg, As, Cd, Cr) in twenty species of natural medicines circulated in Korea and China. Methods : The levels of heavy metal ions(Pb, As, Cd, Cr) were processed using kjeldahl digestion and ICP(Inductively Coupled Plasma spectometer). Mercury was determined by the amalgamation method. Results : Lead was detected less than 0.01(㎍/g) in most samples but in Achyranthes Root it was detected more than 1.0(㎍/g). The amount of Mercury was 0.01(㎍/g) in Trichosanthis Radix and 0.14(㎍/g) in Lonicera Flower. However, in Osterici Koreani Radix of Seong-do(China), Coix Seed of Jecheon(Korea) and Seoul(Korea) and Cimicifuga Rhizome of Seong-do(China), it was detected more than 1.2(㎍/g). The amount of Arsenic was determined 0.12(㎍/g) in Peach Kernel and 0.89(㎍/g) in Cimicifuga Rhizome. Cadmium was detected similar levels in most samples. Cadmium levels in Asiasarum Root was higher than any other samples and its value was 0.40(㎍/g). The amount of Chromium was detected 0.26(㎍/g) in Coix seed and 4.1(㎍/g) in Asiasarum Root. Chromium levels in Osterici Koreani Radix, Achyranthes Root and Asiasarum Root were higher than others. Hazardous heavy metals except Cr were detected less than 1.0(㎍/g) in most samples. Conclusion : The results mean that natural medicines were exposed to risk-hazardous environment.
Dissolution method development and validation for combination of meloxicam and paracetamol tablets (2014)

The aim of this investigation was to develop and validate a dissolution method for combination of ibuprofen and paracetamol tablets using UV spectrophotometric method. The analytical method was developed by UV spectrophotometry using absorbance ratio method which involves the measurement of absorbance at two wavelengths 243 nm as the λmax of ibuprofen and 213.8 as isoabsorbtive point. The method was validated according to ICH guidelines which include accuracy, precision, specificity, linearity and analytical range. In addition, stability and solubility of both the drugs in different media i.e., water and phosphate buffer of different pH were studied. Based on this, the established dissolution conditions were 900 ml of 0. 2 M phosphate buffer pH 7.2 as dissolution medium at 37±0.5 °C, using USP apparatus II at a stirring rate of 75 rpm for 1 hr. The corresponding dissolution profiles were constructed and all the selected brands showed more than 80% drug release within 45 minutes. Thus, the proposed dissolution method and analytical method can be applied successfully for the Quality control of ibuprofen and paracetamol in marketed tablets.
Planar Star-Shaped Organic Semiconductor with Fused Triphenylamine Core for Solution-Processed Small-Molecule Organic Solar Cells and Field-Effect Transistors (2012)

The symmetrical planar star-shaped organic semiconductor DMM-TPA-[DTS(BTTh(3))](3) with fused TPA core donor and three branch motifs, DTS(BTTh(3)), exhibited efficient p-type semiconducting performance in solution-processed SMOSCs and OFETs compared to the tilted star-shaped TPA-[DTS(BTTh(3))](3); a noteworthy PCE value of 4.16% was observed, with a hole mobility and on/off ratio of 7.6 × 10(-3) cm(2) V(-1) s(-1) and 5 × 10(6), respectively.
Cast Films from Soy Protein Isolates and Fractions (1997)

ABSTRACT Glycerol‐plasticized soy protein films were cast from alkaline aqueous film‐forming solutions of laboratory‐prepared 7S, 11S, and soy isolate (LSI) fractions and from commercial soy isolate (CSI). Tensile strength (TS), elongation at break (E), water vapor permeability (WVP), total soluble matter (TSM), protein solubility (PS), and Hunter L, a , and b color values of these films were determined. The 11S films had greater TS than 7S films ( P < 0.05), while LSI films had greater TS than CSI films ( P < 0.05). No significant differences were detected among mean E values and among mean WVP values of all films ( P > 0.05). The 7S films had higher TSM and PS values than 11S films ( P < 0.05). CSI films were significantly darker (lower L value) and more yellow (greater positive b value) than LSI films ( P < 0.05).
Generation of biologically active anti-<i>Cryptococcus neoformans</i> IgG, IgE and IgA isotype switch variant antibodies by acridine orange mutagenesis (1996)

Administration of MoAbs to Cryptococcus neoformans capsular glucuronoxylomannan (GXM) can alter the course of infection in mouse models. However, the effectiveness of these antibodies appears to depend on isotype and specificity. Comparison of isotype protection efficacy requires families of MoAbs with identical fine specificity and different constant region domain. The generation of such families by hybridoma technology is not always possible because the immune response produces MoAbs of limited classes or subclasses. In these instances isotype switch variants can be isolated in vitro. Unfortunately, standard methods of recovering spontaneous switch variants are often unsuccessful, mainly because of the low frequency of switching. In this study we demonstrate that acridine orange stimulation of an IgG3 anti-C. neoformans-producing hybridoma can be used to recover the entire set of isotype switch variants: IgGl, IgG2b, IgG2a, IgE and IgA. All isotype switch variants bind to GXM; fine specificity mapping, using an 11 amino acid peptide polysaccharide mimetope, revealed conservation of binding site specificity. Furthermore, all isotype switch variants reacted with an anti-idiotopic MoAb. The functional activity of this set of MoAbs was demonstrated by their ability to enhance phagocytosis and antifungal efficacy of human macrophage-like THP-1 cells, with IgG3 being the most effective and IgE being the least effective.
ENTUSIASMO ESTUDIANTIL EN EL II RALLIE ATLETICO CULTURAL A PIE (1991)

CON LA PARTICIPACION DE 75 EQUIPOS, DE CUATRO ELEMENTOS CADA UNO, SE LLEVO A CABO POR SEGUNDO ANO CONSECUTIVO, EN CIUDAD UNIVERSITARIA, EL RALLIE ATLETICO CULTURAL A PIE, COMPETENCIA ESPECIAL INCLUIDA EN EL PROGRAMA DEPORTE Y RECREACION PARA LA FORMACION INTEGRAL DEL ESTUDIANTE UNIVERSITARIO.
Dielectric Properties of (Ba, Sr)TiO<sub>3</sub> Thin Films Deposited by RF Sputtering (1993)

Thin films of (Ba 0.65 Sr 0.35 )TiO 3 (BST) have been prepared by an rf-sputtering method at substrate temperatures of 500 to 700°C. The dielectric constant of these films ranges from 190 to 700 at room temperature. This value changes with the grain size rather than the film thickness. The dielectric constant of about 300 and leakage current density of about 1×10 -8 A/cm 2 are obtained in the 65-nm-thick film deposited at a substrate temperature of 600°C. This shows the BST film can be applied to dielectrics of dynamic random access memory (DRAM) capacitors.
Studies on the Polymorphism of L-Glutamic Acid (1963)

The β-crystal formation of L-glutamic acid in the seeded solution was investigated; and it was found that the growth rate of the seed crystals in a-axis direction was nearly as large as that of the α-crystal, but the growth rate in b- and c-axes was little recognized. The activation energy of the crystallization process of the β-crystal in a-axis direction was calculated from the growth rate constants determined at various temperatures, and 6-7kcal/mol was obtained. On the assumption that the crystallization of β-crystal growth was controlled by the diffusional operation, the thickness of the laminar film was calculated from the growth rate constant and the estimated value of the diffusional constant. The calculated value of the thickness was much greater than the value reported by Nernst; therefore, the crystallization process should be controlled by the surface reaction. The co-existence of a small quantity of amino acids caused a great reduction in the growth rate of the β-crystal.
[3H]biotin-labeled proteins in cultured human skin fibroblasts from patients with pyruvate carboxylase deficiency. (1983)

Biotin containing carboxylases in cultured human skin fibroblasts were radioactively labeled by addition of [8,9-3H]biotin to biotin-depleted cell cultures. Three major bands were visualized by fluorography after sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the fibroblast proteins. These bands corresponded to pyruvate carboxylase (Mr = 125,000), the biotin-containing subunit of methyl crotonyl-CoA carboxylase (Mr = 75,000) and the biotin-containing subunit of propionyl-CoA carboxylase (Mr = 73,000) as judged by molecular weight markers, purified carboxylase protein standards, and interaction with monospecific antisera. Four out of 5 cell lines from patients with classical pyruvate carboxylase deficiency (less than 5% of normal activity) labeled with this technique displayed a normal band in the position of pyruvate carboxylase while one cell line showed complete absence of any labeled protein in this area. These results demonstrate heterogeneity in the etiology of pyruvate carboxylase deficiency.
A Systematic Approach to Efficiently Managing the Effects of Retroactive Updates of Time-varying Data in Multiversion XML Databases (2017)

A retroactive update is an update that changes a past data. It is a common operation in both conventional and temporal databases. However, in temporal databases, a retroactive update is challenging since it could lead to data inconsistencies if the retroactively updated data were used for creating other data (like social contributions and taxes which are calculated based on the salaries of the employees). Such data inconsistencies must be repaired in order to preserve the database consistency. In this paper, we extend our previous approach on detecting and repairing automatically data inconsistencies that result from retroactive updates of multiversion temporal XML databases. The extension consists in: 1) providing an enhanced version of the architecture of our approach and explaining the process of handling a retroactive update; 2) showing how to extract data dependencies and how to use them in order to repair detected inconsistencies; 3) proposing a new log structure ensuring a complete and useful history of the executed transactions; 4) presenting a tool, named Retro-Update-Manager, that we have developed to prove technically our approach.
Spatial multiplex in downlink multiuser multiple-antenna wireless environments (2004)

The paper studies the optimal spatial multiplexing scheme in a downlink multiple-antenna environment with perfect transmitter and receiver channel knowledge. Using recent results on the sum capacity of the Gaussian vector broadcast channel, the optimal number of precoded data streams in a downlink channel is characterized. The main result is the following: the sum-capacity achieving transmission strategy in a random downlink channel with n transmit antennas at the base-station and K receivers each equipped with m antennas involves between n and 1/2 n(n+1) data streams in total, with each user receiving between m and 1/2 m(m+1) data streams. This gives a dimension counting interpretation for multiuser diversity. In particular, it shows that the throughput maximizing transmission strategy in a downlink channel with n transmit antennas should involve between n and 1/2 n(n+1) active users at any time.
Optimal Consumption for General Diffusions with Absorbing and Reflecting Barriers (1984)

Two stochastic control problems of the storage or inventory type are considered for general diffusion processes. In the absorption problem, a diffusion process is controlled by subtracting a nondecreasing withdrawal process. The controlled process is absorbed if it reaches zero. The objective function to be maximized is the expected discounted value of the withdrawals plus a discounted penalty for absorption. In the reflection problem, the process can be controlled by subtracting a withdrawal process and adding a deposit process, and the controlled process must be nonnegative. One seeks to maximize an expected discounted weighted difference in withdrawals and deposits. The value function is computed, and a necessary and sufficient condition for the existence of an optimal policy is given. When they exist, optimal policies are found to be the minimal processes which keep the controlled process inside an interval.