gebhart/patrepeval huggingface dataset:
Nanoparticle‐filled capillary electrophoresis for the separation of long DNA molecules in the presence of hydrodynamic and electrokinetic forces (2005)

We report the analysis of long DNA molecules by nanoparticle-filled capillary electrophoresis (NFCE) under the influences of hydrodynamic and electrokinetic forces. The gold nanoparticle (GNP)/polymer composites (GNPPs) prepared from GNPs and poly(ethylene oxide) were filled in a capillary to act as separation matrices for DNA separation. The separations of lambda-DNA (0.12-23.1 kbp) and high-molecular-weight DNA markers (8.27-48.5 kbp) by NFCE, under an electric field of -140 V/cm and a hydrodynamic flow velocity of 554 microm/s, were accomplished within 5 min. To further investigate the separation mechanism, the migration of lambda-DNA was monitored in real time using a charge-coupled device (CCD) imaging system. The GNPPs provide greater retardation than do conventional polymer media when they are encountered during the electrophoretic process. The presence of interactions between the GNPPs and the DNA molecules is further supported by the fluorescence quenching of prelabeled lambda-DNA, which occurs through an energy transfer mechanism. Based on the results presented in this study, we suggest that the electric field, hydrodynamic flow, and GNPP concentration are the three main determinants of DNA separation in NFCE.
Estrogen Pharmacology. II. Suppression of Experimental Immune Polyarthritis (1964)

SummaryEstradiol and its transformation products, estrone and estriol, markedly diminish the incidence and severity of the inflammatory polyarthritis induced by adjuvant injection in rats. These natural estrogens confer protection whether administered before inoculation or during the latent period of the disease, suggesting that they may act in part to suppress the capacity of the involved tissues to react to the immunologic inflammatory stimulus in this disorder. These observations are consistent with others which indicate that estrogens and/or pregnancy may alter certain expressions of immunoogical responsiveness in man and experimental animals.
High Temperature Superconductivity (1990)

Like astronomers tweaking images to gain a more detailed glimpse of distant stars, physicists at Brookhaven National Laboratory have found ways to sharpen images of the energy spectra in high-temperature superconductors.
Labeling of the glycoprotein subunit of sodium-potassium ATPase with fluorescent probes (1985)

Sodium plus potassium activated adenosinetriphosphatase [(Na,K)ATPase] is composed of a catalytic subunit (alpha) and a glycoprotein subunit (beta) of unknown function. A method has been developed to label the beta subunit of purified dog kidney (Na,K)ATPase with fluorescent probes. The method consists of oxidation of beta-subunit oligosaccharides, reaction of the resulting aldehydes with fluorescent hydrazides, and reduction of the hydrazones and unreacted aldehydes with NaBH4. Two oxidation methods were compared. Simultaneous treatment with neuraminidase and galactose oxidase did not inhibit significantly (Na,K)ATPase activity and allowed insertion of up to 11 mol of probe per mol of beta. In contrast, oxidation of (Na,K)ATPase oligosaccharides with periodate resulted in 50-80% inhibition of the (Na,K)ATPase activity with low or undetectable labeling. Eleven commercial probes and two novel hydrazides were tested for labeling of (Na,K)ATPase treated with galactose oxidase and neuraminidase. Eight probes did not label (Na,-K)ATPase but labeled red cell ghosts oxidized with periodate. Four probes labeled beta specifically but either adsorbed to the membrane tightly, or cross-linked the beta subunits, or formed unstable adducts. Lucifer yellow CH labeled beta specifically without membrane adsorption. Labeling stoichiometries from 1 to 11 mol of lucifer yellow CH per mol of beta were obtained without inhibition of (Na,K)ATPase activity and without significant alteration of the anthroylouabain binding capacity or its association and dissociation kinetics. Anthroylouabain specifically bound to the lucifer-labeled (Na,K)ATPase had a decreased quantum yield, probably due to resonance energy transfer. This suggests that the sites of lucifer attachment on beta are within energy transfer distance from the cardiac glycoside site on alpha.(ABSTRACT TRUNCATED AT 250 WORDS)
Ursolic Acid as a Trypanocidal Constituent in Rosemary. (2002)

The MeOH extract of the leaves of rosemary (Rosmarinus officinalis L.) completely inhibited the motility of cultured epimastigotes of Trypanosoma cruzi at the concentration of 2 mg/ml after 2 h of incubation. Activity-guided fractionation of the MeOH extract has resulted in the isolation of three triterpene acids, betulinic, oleanolic and ursolic acids. Ursolic acid stopped the movement of all T. cruzi epimastigotes at the minimum concentration (MC(100)) of 40 micro g/ml (88 micro M) after 48 h of incubation. Oleanolic acid was less active (MC(100): 250 micro g/ml, 550 micro M) and betulinic acid was practically inactive.
Caroli's disease and congenital hepatic fibrosis associated with polycystic kidney disease. A case presenting with acute focal bacterial nephritis. (1992)

Congenital cystic dilatation of the intrahepatic biliary ducts (Caroli's disease), until recently, has been infrequently recognized. It is often associated with autosomal recessive polycystic kidney disease (ARPKD) and congenital hepatic fibrosis (CHF). We hereby report a case with Caroli's disease, polycystic kidney disease (PKD), and CHF: This 24-year-old female patient initially presented with acute bacterial nephritis (ABN). Renal ultrasonography revealed bilateral enlarged kidneys with multiple cysts. Because her parents showed no renal cyst on ultrasonographic examination, she received further studies. Abdominal ultrasonography showed cystic dilatation of the biliary tree. Computed tomography (CT) with meglumine lotroxinate (biliscopin) infusion study and hepatobiliary scintigraphy confirmed the diagnosis of Caroli's disease. Liver biopsy revealed CHF: The radiographic and scintigraphic pictures are hereby illustrated and CT with biliscopin infusion study is emphasized. We conclude that if radiologic evidence of renal cystic lesions is absent in the parents of patients with PKD, the coexistence of Caroli's disease and CHF should be considered. The clinical pictures of ABN in this patient are also discussed. As far as we know, this is the first reported case of ABN in a patient with PKD and Caroli's disease, and it showed good response to antibiotic therapy.
Development of vocal tract length during early childhood: A magnetic resonance imaging study (2005)

Speech development in children is predicated partly on the growth and anatomic restructuring of the vocal tract. This study examines the growth pattern of the various hard and soft tissue vocal tract structures as visualized by magnetic resonance imaging (MRI), and assesses their relational growth with vocal tract length (VTL). Measurements on lip thickness, hard- and soft-palate length, tongue length, naso-oro-pharyngeal length, mandibular length and depth, and distance of the hyoid bone and larynx from the posterior nasal spine were used from 63 pediatric cases (ages birth to 6 years and 9 months) and 12 adults. Results indicate (a) ongoing growth of all oral and pharyngeal vocal tract structures with no sexual dimorphism, and a period of accelerated growth between birth and 18 months; (b) vocal tract structure's region (oral/anterior versus pharyngeal/posterior) and orientation (horizontal versus vertical) determine its growth pattern; and (c) the relational growth of the different structures with VTL changes with development-while the increase in VTL throughout development is predominantly due to growth of pharyngeal/posterior structures, VTL is also substantially affected by the growth of oral/anterior structures during the first 18 months of life. Findings provide normative data that can be used for modeling the development of the vocal tract.
Reduced systemic drug exposure by combining intraarterial cis-diamminedichloroplatinum(II) with hemodialysis of regional venous drainage. (1987)

During cancer chemotherapy toxicity to normal tissues often limits the tolerable dose. To increase drug delivery to tumor while maintaining tolerable systemic exposure, regional treatments, such as intraarterial drug delivery, have been used. Despite intraarterial delivery, systemic toxicity often remains the dose-limiting sensitivity. If systemic drug exposure could be reduced after intraarterial infusion, the intraarterial dose could be increased, which should increase the therapeutic response. We compared the pharmacokinetic advantage after cisplatin infusion into the internal carotid artery to that obtained after infusing cisplatin into the internal carotid artery during extracorporeal removal of cisplatin from the jugular blood by hemodialysis. Four patients with malignant gliomas received intracarotid cisplatin, 100 mg/m2 over 60 min, every 4 weeks. During one treatment, while cisplatin was infused into the internal carotid artery, the jugular blood was dialyzed extracorporeally at 300 ml/min and returned to the inferior vena cava. Seventy to 96% of the free platinum that entered the dialyzer was removed. By aspirating blood from the jugular vein at 300 ml/min, 30-79% of the ipsilateral carotid blood was collected for extracorporeal circulation. Hemodialysis of the cerebral venous drainage during intracarotid infusion reduced the systemic exposure to cisplatin by 51-61% when compared to the exposure from internal carotid artery infusion without hemodialysis. The pharmacokinetic advantage (brain/body exposure ratio) was increased from 3 to 5/1 during internal carotid artery infusion alone to as much as 15/1 during treatment combining intracarotid infusion with hemodialysis of the jugular blood. Systemic toxicity now limits the dose of cisplatin that can be administered safely. Increased tumor exposure without increased systemic toxicity may be possible with the technique described and greater doses of cisplatin. Assuming no associated local toxicities, the results of the current study indicate that the dose of intracarotid cisplatin can be increased while maintaining tolerable systemic exposure.
Safe-Keeping Digital Evidence with Secure Logging Protocols: State of the Art and Challenges (2009)

While log data are being increasingly used as digital evidence in court, the extent to which existing secure logging protocols used to collect log data fulfill the legal requirements for admissible evidence remain largely unclear. This paper elucidates a subset of the necessary secure requirements for digital evidence and extensively surveys the state of the art secure logging protocols, thereby demonstrating that none of the current protocols completely fulfills the elucidated requirements for admissible evidence. In analyzing the shortcoming of logging protocols, the paper also elaborates on the related research challenges.
Molecular Basis of Different Forms of Metachromatic Leukodystrophy (1991)

Like many lysosomal storage disorders, metachromatic leukodystrophy shows clinical heterogeneity that seems to reflect genetic heterogeneity. One of the known alleles (allele I) is associated with earlier and more severe disease than the other (allele A).
Elongation Factor-2 Kinase: Its Role in Protein Synthesis and Autophagy (2006)

Elongation factor-2 kinase (eEF-2 kinase; Ca(2+)/calmodulin-dependent kinase III) controls the rate of peptide chain elongation. The activity of eEF-2 kinase is increased in many malignancies, yet its precise function in carcinogenesis remains unknown. Autophagy, a well-defined survival pathway in yeast, may also play an important role in oncogenesis. Furthermore, the autophagic response to nutrient deprivation is regulated by the mammalian target of rapamycin (mTOR). eEF-2 kinase lies downstream of mTOR and is regulated by several kinases in this pathway. Therefore, we studied the role of eEF-2 kinase in autophagy. Knockdown of eEF-2 kinase by RNA interference inhibited autophagy in several cell types as measured by light chain 3 (LC3)-II formation, acidic vesicular organelle staining, and electron microscopy. In contrast, overexpression of eEF-2 kinase increased autophagy. Furthermore, inhibition of autophagy markedly decreased the viability of glioblastoma cells grown under conditions of nutrient depletion. These results suggest that eEF-2 kinase plays a regulatory role in the autophagic process in tumor cells and may promote cancer cell survival under conditions of nutrient deprivation. Therefore, eEF-2 kinase activation may be a part of a survival mechanism in glioblastoma, and targeting this kinase may represent a novel approach to cancer treatment.
Probability of valid gait data acquisition using currently available force plates. (1997)

A major difficulty in using force plates for gait analysis is to have a foot fall completely on the instrument while not having the other foot in contact with the same device. This translates into more trials (and time) required to obtain valid data. In order to estimate the probability of a successful trial, which is related to the overall number of trials required, the dimensional relationships between force plate, foot and gait cycle were investigated. In particular, it was analyzed how the dimensions of the plate, the size of foot, the step length and width affect the probability of having a successful trial when acquiring one or two subsequent foot falls using one or two force plates respectively. The equations obtained can also be used to estimate the force plate dimensions that allow for the minimum number of trials for a specific group of subjects. To illustrate this approach, commercially available force plate sizes were statistically evaluated using gait data collected in recent years at The Ohio State University Gait Laboratory from patients with different pathologies. The force plate length maximizing the probability of a successful trial was identified for this specific population.
Ovarian Cancer Screening (2012)

Annual screening of asymptomatic postmenopausal women using a combination of transvaginal sonography and serum Ca-125 has been evaluated in 4 major clinical trials as a means to facilitate earlier detection of ovarian cancer. In several trials, screening has caused a decrease in stage at detection. These ovarian cancer screening trials are reviewed. The effect of screening on ovarian cancer mortality varies in different trials and is the subject of further investigation.
Acid Labile Crosslinked Units: A new Concept for Deep-UV Photoresists. (1996)

A new type of acetal protected hydroxystyrene polymers with acid labile crosslinked units has been developed for KrF excimer laser photoresists. Deep-UV photoresist formulations based on these new polymers provide better resolution and higher thermal flow stability than conventional systems. The polymer structure was evaluated by 13C-NMR spectroscopy and gel permeation chromatography. Factors controlling the crosslinking reactions and the impact of the crosslinking degree on lithographic properties are discussed.
<i>Haemophilus haemolyticus:</i> A Human Respiratory Tract Commensal to Be Distinguished from <i>Haemophilus influenzae</i> (2006)

Standard methods do not reliably distinguish H. haemolyticus from H. influenzae. H. haemolyticus is a respiratory tract commensal. The recognition that some strains of apparent H. influenzae are H. haemolyticus substantially strengthens the association of true H. influenzae with clinical infection.
Application-aware deadlock-free oblivious routing (2009)

Conventional oblivious routing algorithms are either not application-aware or assume that each flow has its own private channel to ensure deadlock avoidance. We present a framework for application-aware routing that assures deadlock-freedom under one or more channels by forcing routes to conform to an acyclic channel dependence graph. Arbitrary minimal routes can be made deadlock-free through appropriate static channel allocation when two or more channels are available. Given bandwidth estimates for flows, we present a mixed integer-linear programming (MILP) approach and a heuristic approach for producing deadlock-free routes that minimize maximum channel load. The heuristic algorithm is calibrated using the MILP algorithm and evaluated on a number of benchmarks through detailed network simulation. Our framework can be used to produce application-aware routes that target the minimization of latency, number of flows through a link, bandwidth, or any combination thereof.
Orthosteric/Allosteric Bitopic Ligands: Going Hybrid at GPCRs (2009)

G protein-coupled receptors (GPCRs) can adopt multiple biologically active states that can be differentially stabilized by ligands that bind to topographically distinct sites (e.g., orthosteric ligands and allosteric modulators). Recent studies in the field are now demonstrating the utility of linking orthosteric and allosteric pharmacophores to yield hybrid, or bitopic, ligands, with improved affinity and/or receptor subtype selectivity. Interestingly, this approach can also engender functional selectivity in the actions of orthosteric ligands, highlighting a viable means of further sculpting GPCR ligand responses. Indeed, some previously identified functionally selective agonists may actually represent hitherto unappreciated bitopic ligands.
Finite Element Analysis for Temperature Distribution of Breast (2007)

A multi-dimension comprehensive thermal model is presented to simulate the steady temperature distribution of breast, which is based on the breast anatomical structure and physiological characteristics. This model takes the metabolic heat generation, blood perfusion and vascular thermal interaction into account. Finite element analysis method is used to solve the heat diffusion equation. The simulated results show that the temperature profiles of breast can be significantly affected by the metabolic heat generation and blood perfusion. Furthermore, the temperature distribution of abnormal breast with tumor is also discussed. The results seem very valuable in the interpretation of thermograms.
HyBi WebSocket Requirements and Features (2011)

This document considers the requirements and resulting features needed
for the design of the WebSocket Protocol. The goal of the document is
to provide a stable base for protocol design and related discussion.
Azithromycin versus tetracycline in the treatment of acne vulgaris (2006)

Azithromycin is a safe and effective alternative in the treatment of inflammatory acne.
Minimizing the required memory bandwidth in VLSI system realizations (1999)

In this paper, we present the problem of storage bandwidth optimization (SBO) in VLSI system realizations. Our goal is to minimize the required memory bandwidth within the given cycle budget by adding ordering constraints to the flow graph. This allows the subsequent memory allocation and assignment tasks to come up with a cheaper memory architecture with less memories and memory ports. The importance and the effect of SBO is shown on realistic examples both in the video and asynchronous transfer-mode (ATM) domains. We show that it is important to take into account which data is being accessed in parallel, instead of only considering the number of simultaneous memory accesses. Our problem formulation leads to the optimization of a conflict (hyper) graph. For the target domain of ATM, only flat graphs without loops have to be treated. For this subproblem, a prototype tool has been implemented to demonstrate the feasibility of automating this important system design step.
Characterization of the growth hormone-binding protein of human serum using a panel of monoclonal antibodies (1989)

A panel of monoclonal antibodies (MAbs) reactive with distinct epitopes on the rabbit liver GH receptor and rabbit serum GH-binding protein (GHBP) were tested for cross-reactivity with the GHBP from human serum. Four of seven MAbs reacted with the human serum GHBP. Immunoprecipitation of the human binding protein enabled hormonal specificity identical to that previously reported for human GH receptors to be demonstrated. Scatchard analyses of 125I-labelled human GH binding to the serum GHBP were carried out with correction made for endogenous human GH which was measured by radioimmunoassay of each serum sample. This approach yielded the first reliable estimates of the affinity and capacity of the human GHBP. The binding capacity (mean +/- S.E.M.) of female sera (804 +/- 126 pmol/l; n = 6) was greater than that of male sera (505 +/- 36 pmol/l; n = 9; P less than 0.02). The affinity of the GHBP was 0.91 +/- 0.10 litres/nmol (n = 15). The presence of multiple epitopes common to the human serum GHBP and the rabbit liver GH receptor is consistent with identity between the extracellular domains of the human GHBP and the human GH receptor, as is the case for the rabbit GHBP and GH receptor.
An Analytic Equation for the Volume Fraction of Condensationally Grown Mixed Particles and Applications to Secondary Organic Material Produced in Continuously Mixed Flow Reactors (2014)

Secondary condensation of organic material onto primary seed particles is one pathway of particle growth in the atmosphere, and many properties of the resulting mixed particles depend on organic volume fraction. Environmental chambers can be used to simulate the production of these types of particles, and the optical, hygroscopic, and other properties of the mixed particles can be studied. In the interpretation of the measured properties, the probability density function <i>p</i>(<i>ϵ</i>;<i>d</i>) of volume fraction <i>ϵ</i> of the condensing material for particle diameter <i>d</i> in the outflow of the chamber is typically needed. In this article, analytic equations are derived <i>p</i>(<i>ϵ</i>;<i>d</i>) for condensational growth in a continuously mixed flow reactor. The equation predictions are compared to measurements for the condensation of secondary organic material on quasi-monodisperse sulfate seed particles. Equations are presented herein for discrete, Gaussian, and triangular distribution functions for the seed particle number–diameter distributions, including generalization to any linearly segmented distributions. The analytic equations are useful both for the interpretation of laboratory data from environmental chambers, such as the construction of probability density functions for use in interpretation of hygroscopic growth data, cloud–condensation–nuclei data, or other laboratory data sets dependent on organic volume fraction, as well as for understanding atmospheric processes at times that condensational growth processes prevail.Copyright 2014 American Association for Aerosol Research