gebhart/patrepeval huggingface dataset:
Components of Promyelocytic Leukemia Nuclear Bodies (ND10) Act Cooperatively To Repress Herpesvirus Infection (2012)

Upon the entry of the viral genome into the nucleus, herpes simplex virus type 1 (HSV-1) gene expression is rapidly repressed by constitutively expressed cellular proteins. This intrinsic antiviral defense is normally counteracted by ICP0, which allows virus infection to proceed efficiently. Replication of ICP0-null mutant HSV-1, however, is severely repressed by mechanisms that are conferred, at least in part, by nuclear domain 10 (ND10) components, including hDaxx, the promyelocytic leukemia (PML) protein, and Sp100. To investigate if these ND10 components repress viral gene expression in a cooperative manner, we simultaneously depleted host cells for hDaxx, PML, and Sp100 by multiple short hairpin RNA (shRNA) knockdown from a single lentivirus vector. We found that replication and gene expression of ICP0-null mutant HSV-1 were cooperatively repressed by hDaxx, PML, and Sp100 immediately upon infection, and all stages of virus replication were inhibited. Plaque-forming efficiency was enhanced at least 50-fold in the triple-depleted cells, a much larger increase than achieved by depletion of any single ND10 protein. Similar effects were also observed during infection of triple-depleted cells with human cytomegalovirus (HCMV). Moreover, using a cell culture model of quiescent infection, we found that triple depletion resulted in a much larger number of viral genomes escaping repression. However, triple depletion was unable to fully overcome the ICP0-null phenotype, implying the presence of additional repressive host factors, possibly components of the SUMO modification or DNA repair pathways. We conclude that several ND10 components cooperate in an additive manner to regulate HSV-1 and HCMV infection.
Decomposition of Silver Carbonate; the Crystal Structure of Two High-Temperature Modifications of Ag<sub>2</sub>CO<sub>3</sub> (2002)

High-resolution powder diffraction was used to study the thermal transformation of silver carbonate. A sample of Ag(2)CO(3) was heated in a capillary under 4.5 atm CO(2) pressure. The decomposition temperature of silver carbonate to silver oxide is thereby increased, allowing high-resolution synchrotron X-ray powder diffraction patterns of the two high-temperature phases of Ag(2)CO(3) to be collected. The structure of the low-temperature (lt) phase was confirmed, and the structures of the two high-temperature phases were determined by direct methods and refined using the Rietveld method: lt-Ag(2)CO(3) (295 K) P2(1)/m, z = 2, a = 4.8521(2) A, b = 9.5489(4) A, c = 3.2536(1) A, beta = 91.9713(3) degrees; beta-Ag(2)CO(3) (453 K) P31c, z = 6, a = 9.1716(4) A, c = 6.5176(3) A; alpha-Ag(2)CO(3) (476 K) P6 macro 2m, z = 3, a = 9.0924(4) A, c = 3.3249(1) A. In addition, thermal expansion properties, anisotropic microstrain distributions, and thermal transformations of the three silver carbonate phases and silver oxide are described.
Combined topological and functionality based delay estimation using at layout-driven approach for high level applications (1992)

The problem of accurate delay estimation of cell-based designs, prior to any physical design tasks, is discussed. For this purpose, accurate wire-length estimates are required, since wire delays contribute significantly to the overall delay. A new technique is presented for wire-length estimation based on a combination of analytical and constructive approaches. Given these wire-length estimates and the cell delays, it is possible to provide worst case delay paths in the design based on the circuit topology. The technique is extended to consider false paths, which provides a more accurate functionality based estimate which takes into account the estimated layout information. The technique is validated using standard MCNC benchmarks. Results indicate a 7% or better accuracy in the worst case delay predictions for designs with up to 1800 cells. >
Protein kinase C phosphorylation of cardiac troponin I or troponin T inhibits Ca2(+)-stimulated actomyosin MgATPase activity. (1991)

Effects of troponin phosphorylation on Ca2(+)-stimulated MgATPase activity of bovine cardiac actomyosin were examined. Phosphorylation by protein kinase C of troponin I and troponin T subunits in troponin or troponin-tropomyosin complex resulted in a decreased Ca2(+)-stimulated MgATPase activity in reconstituted actomyosin, and this effect was reversed by subsequent dephosphorylation by protein phosphatase 1. It was further observed that protein kinase C phosphorylation of either troponin I or troponin T subunits led to a similar inhibition of Ca2(+)-stimulated actomyosin MgATPase activity. In all cases, EC50 values (concentrations causing 50% stimulation) for Ca2+ were not appreciably affected by troponin phosphorylation by protein kinase C. Data from phosphorylation site analysis suggests that phosphorylation of threonine 144 in troponin I and possibly threonine 280 or threonine 199 in troponin T might be important for the observed decrease of Ca2(+)-stimulated actomyosin MgATPase. It is suggested that inhibition of actomyosin MgATPase caused by protein kinase C phosphorylation of troponin I and/or troponin T represents a new mechanism that can account for in part the reported negative inotropic effect of phorbol esters on various cardiac preparations.
Multi objective optimization of part orientation in stereolithography (2009)

This paper presents a Pareto Based Optimization Algorithm to determine the best part orientation in Stereolithography systems. The previous methods could either handle simple parts or limited Objective Functions (OF). Moreover the methods with multiple OFs, had abstracted them into a single fitness function. A single fitness function never reflects the characteristics of individual OFs properly. The method proposed here handles several OFs individually. The objective functions are minimum build time and minimum support volume under desired surface finish. The optimization is done using Genetic Algorithm (GA). At each GA step, the surface finish is achieved applying adaptive layer thickness method. Pareto Based Optimization (PBO) finds the best part orientation with minimum build time and volume support. PBO is a multi objective optimization to handle complex CAD files. The algorithm is developed by MATLAB. The codes are run for some case studies. The results are very promising.
Honoring Students (2011)

Abstract Starting school for children marks the first time they will be evaluated outside the home. Infancy and early childhood tend to be spent with family or in daycare settings where people know you as an individual, and aren't especially judgmental. In contrast, school subjects children to expectations and evaluations, evidenced by graded schoolwork and report cards. Most family members marvel at how much small children learn during their preschool years. But school brings together lots of these children — many regarded as wonderfully bright by their families — and the school's teachers don't have the same intense emotional ties to those kids. Suddenly, it becomes much easier to compare children, and parents are confronted with how teachers — representatives of the larger world — evaluate their youngsters. School, then, is an arena where children can gain status, not as family members who are known and loved as unique individuals but as students.
Reviews (1966)

Journal Article Reviews Get access CONRAD'S POLISH BACKGROUND: LETTERS TO AND FROM POLISH FRIENDS , edited by Zdzislaw Najder and translated by Halina Carroll . London : Oxford University Press , 1964 ; pp. viii, 313 ; 45 s . NORMAN SHERRY NORMAN SHERRY Search for other works by this author on: Oxford Academic Google Scholar Notes and Queries, Volume 13, Issue 3, March 1966, Pages 109–110, https://doi.org/10.1093/nq/13-3-109 Published: 01 March 1966
Reviewing the health of software ecosystems – a conceptual framework proposal (2013)

Abstract. The health of a software ecosystem is an indication of how well the ecosystem is functioning. The measurement of health can point to issues that need to be addressed in the ecosystem and areas for the ecosystem to improve. However, the software ecosystem field lacks an applicable way to measure and evaluate health. In this work, we review the literature related to the concept of software ecosystem health and the literature that inspired the software ecosystem health literature (a total of 23 papers) and (i) identify that the main source of inspiration is the health of business ecosystems while also influenced by theories from natural ecosystems and open source, (ii) identify two areas where software ecosystems differ from business and natural ecosystems, and (iii) propose a conceptual framework for defining and measuring the health of software ecosystems. Key words: software ecosystems, ecosystem health, software ecosystem health framework, software ecosystem health measurement
[Construction of eukarocyte expression recombinant of human thymosin alpha1 and its influnce to immune activity of PBLs transfected with the recombinant pBK-T alpha1] (2002)

Objective: To construct pBK Tα1, the eukarocyte expression recombinant of human thymosinα1 and to study its influence to immune activity of human peripheral blood lymphocytes(PBLs) transfected with pBK Tα1 . Methods: Tα1 gene was prepared by digesting pBK Tα1 with EcoR I and Hind Ⅲ. Then, pBK Tα1 was produced by inserting Tα1 into pBK CMV. Finally PBLs were transfected with pBK Tα1 by FUGENETM 6 transfection method. The pBK Tα1 transcript in PBLs was confirmed by RT PCR. The erythrocyte rosette test (Et test) was used to detected E surface receptors on T lymphocytes. IFN γ and IL 2 were then measured in the supernant of PBLs transfected with pBK Tα1. Results: pBK Tα1, the eukarocyte expression recombinant of human thymosinα1, has been successfully constructed. At the transcription level, the recombinant pBK Tα1 can transcript mRNA of Tα1. The transfected PBLs with pBK Tα1 increased the expression of IFN γ, IL 2 and the production of E surface receptors on T lymphocytes. Conclusion: The expression of pBK Tα1 may promote immune function of PBLs.
Chronic cryptococcal meningitis: a new experimental model in rabbits. (1980)

This paper describes the salient features of a new model for chronic cryptococcal meningitis in cortisone-treated rabbits. Normal rabbits soon recovered after intracisternal inoculation of Cryptococcus neoformans, but cortisone-treated animals developed chronic progressive meningitis that was fatal in 2-12 weeks. Incidence and severity of infection was related to cortisone dose, not to inoculum size. The number of mononuclear cells that migrated into the subarachnoid spaces and cerebrospinal fluid of infected rabbits was strikingly reduced by cortisone treatment. Rabbits with cryptococcal meningitis were febrile; their high body temperature did not confer resistance to this infection. Cortisone-treated rabbits provide a new and expedient laboratory model for cryptococcal disease. Potential applications include study of the pathogenesis of cryptococcosis, investigation of the immunobiology of the CNS in chronic meningitis, and in vivo evaluation of newer anticryptococcal treatment regimens.
Phase-based Binding Conditions with special reference to Control and Obviation in Spanish and Korean (2012)

On the basis of obviation and control effects in Spanish and Korean finite clauses, I argue in this paper that the pronominal coreference relations are not all accounted for by the Binding Principle B, defined in terms of the relevant local domain D (i.e. TP). To resolve this, I propose a Phase-based Binding Conditions, in which the local domain D is restated in terms of phase domain. It is argued that the volitional subjunctives in the obviation and control structures have defective T, since they are sharply dependent on the matrix T. I assume that the CP with defective T is a weak phase CP, which cannot act as a phase for the binding interpretation. According to the Condition B, the null pronominal pro in obviation structure must be interpreted as disjoint from every c-commanding phrase in its phase, the matrix vP. The null anaphoric pro in control structure must be interpreted as coreferential with a c-commanding phrase in its phase, the matrix vP, as well, in compliance with the Condition A. In sum, this paper tries to account for the anaphoric property of the controlled pro (or PRO), reducing the distribution of pro (or PRO) to the Condition A by considering them just as a null anaphor.
Germ Line Polymorphism in Metastatic Progression (2006)

Somatic genetic analysis of tumors and metastases has yielded a plethora of information regarding genes associated with cancer progression. However, somatic alterations in tumor cells are only one source of variability. Genetic polymorphism may also play a significant role in person-to-person variability in metastasis frequency, raising the intriguing possibility that some individuals could be predisposed to secondary tumor development. The identification and characterization of these polymorphisms may have significant implications for the development of tailored treatment or prevention of recurrent disease.
Study on the p53 gene in human hepatoma cell line QGY-7703 and its expression (1997)

p53 gene in human hepatoma cell line QGY-7703 was studied with following methods: 17p alleles analysis, PCR-SSCP, RT-PCR/sequencing, Northern blot, immunohistochemistry, Western blot and immunoprecipitation. There may exist allelic loss in the vicinity of p53 gene on chromosome 17p. Whereas no any point mutations were found in the coding region of p53 gene in the cell, Northern blot and immunoprecipitation experiments showed its expression is quite low, which may be relative with the malignancy of the cell.
First Synthesis of 2‐Vinylindole and its <i>diels‐Alder</i> Reactions with CC‐Dienophiles (1988)

Abstract By means of an intramolecular Wittig process, 2‐vinylindole ( 2 ) was prepared. The indole 2 functions as a heterocyclic, donor‐activated 1,3‐diene and undergoes [4+2] cycloaddition reactions with dimethyl acetylenedicarboxylate, N ‐phenylmaleimide, and p ‐benzoquinone leading to the novel carbazole dervatives 3 , 4 , 5c , 6 and 7 respectively. The reaction of 2 with acceptor (A)‐Substituted dienophiles ( e.g. ) ACHCH 2 , AC≡(CH) does not yield products that can be isolated.
Site-directed mutagenesis of amino acid residues of protein phosphatase 1 involved in catalysis and inhibitor binding (1997)

Site-directed mutagenesis of selected residues of mammalian protein phosphatase 1 (PP-1) has been carried out to further define the mechanism of catalysis, activation by divalent cations, and inhibition by toxins and inhibitory proteins. Mutation of active site residues predicted to bind metals (N124D and H248N) resulted in a large loss of enzyme activity and decreased affinity for metal ions; mutation of residues predicted to bind phosphosubstrate (R96A or R221S) led to a large loss of enzyme activity; and mutation of active site residues (D95A and D208A) resulted in a large loss of enzyme activity. Mutants N124D, H248N, R96A, and R221S exhibited large decreases in sensitivity to the toxins calyculin A, okadaic acid, and microcystin and to thiophospho-DARPP-32. Mutation of Y272 (Y272F) had little effect on activity but resulted in a large decrease in sensitivity to okadaic acid and calyculin A. Mutant D208A exhibited a decrease in sensitivity to okadaic acid and calyculin A, but, paradoxically, the sensitivity to inhibition by thiophospho-DARPP-32 was increased. Mutation of acidic groove residues (E256R, E275R, E252A:D253A, and E252A:D253A:E256R) exhibited little change in enzyme activity and no change in sensitivity to toxins, but increased sensitivity to thiophospho-DARPP-32. These results suggest that toxins and phospho-DARPP-32 interact at the active site of PP-1 in a similar fashion despite their differences in structure. In addition, acidic groove residues appear to influence the interaction of the phosphoinhibitor with the active site of PP-1.
The effect of graded inclusion of baked beans (Phaseolus vulgaris) on plasma and liver lipids in hypercholesterolaemic pigs given a Western-type diet (1993)

The aim of the present study was to measure the effect of graded inclusion of baked beans (Phaseolus vulgaris) on plasma and liver lipids in hypercholesterolaemic pigs fed on a Western-type diet. Twenty-four Large White x Landrace pigs of about 30 kg body weight were made hypercholesterolaemic by feeding a semi-purified diet, high in saturated fat and supplemented with 10 g cholesterol/kg, for 14 d. After that, six pigs were randomly assigned to one of the four experimental groups. They received their respective diets, containing 0, 100, 200 or 300 g baked beans/kg, on a dry-matter basis, for a further 28 d. Fasting blood samples were taken and analysed for total plasma cholesterol, lipoproteins and triacylglycerols. After the pigs were slaughtered at the end of the study, livers were analysed for their cholesterol content. Consumption of baked beans at 100, 200 and 300 g/kg reduced plasma total cholesterol by 5.3, 20.2 and 35.6% respectively. However, only the diet with 300 g baked beans/kg showed a significant reduction (P < 0.05) compared with the control (without baked beans). The level of low-density-lipoprotein-cholesterol was also significantly (P < 0.05) reduced by 48% at 300 g baked beans/kg. Plasma very-low-density-lipoprotein-cholesterol, high-density-lipoprotein-cholesterol and triacylglycerol contents were not affected by bean consumption. The supplements of 200 and 300 g baked beans/kg promoted a significant (P < 0.05) reduction of about 50% in cholesterol deposition in the liver, compared with the control.
Mixing machine learning and optimization for the tactical capacity planning in last-mile delivery (2021)

Tactical Capacity Planning (TCP) is becoming a crucial part of logistics in the current environment of demand-driven economics. This paper proposes an innovative approach in the TCP setting, consisting of using the collected historical data of the geographical position and the volume of the orders to plan the capacity requirements for the next day. To this end, the clustering of the city to microzones is introduced using K-means clustering. Then, four different methods (Gaussian Process regression, ARIMA model, Neural Network regression, and Long Short Term Memory network) are used to forecast the next day order volume for each of the clusters. Finally, the Variable Cost and Size Bin Packing problem solved with the predicted demand to outline the usage of a heterogeneous fleet required to serve the next time period. Through experiments on the real data, we conclude, that the proposed algorithm is satisfying the decision safety framework with completely unknown demand and could also be used for other demand forecast applications.
Effects of Imazapic on Target and Nontarget Vegetation during Revegetation (2007)

Medusahead is an introduced, winter-annual grass covering millions of hectares of the semiarid West. It forms exclusive stands and has a dense thatch cover that resists the establishment of desirable species. Prescribed fire can remove medusahead litter and improve plant establishment. Medusahead control is fundamental to establishing desirable species that will, in turn, resist further invasion. Imazapic is an effective herbicide for control of medusahead, but more information is needed on its effects on desirable species. Our objectives were to test how imazapic application rate and timing affected medusahead, seeded desirable species, and other nontarget vegetation on burned and unburned rangeland in southeast Oregon. We burned existing medusahead infestations at two different sites in June 2003. Following the burn, imazapic was applied at rates of 0, 35, 70, 105, 140, 175, and 210 g ai/ha between July and October of 2003 in a randomized strip-plot design. In November 2003, monocultures of seven desirable species were drill-seeded across the imazapic treated areas. Data on cover and density of medusahead and seeded species were collected in 2004 and 2005. Cover data of nontarget species were collected in the summer of 2005. Medusahead cover was highest in control plots and lowest in plots that received the highest herbicide application rates. Medusahead cover was lower in burned plots. The effect of imazapic on nontarget vegetation was less clear. Seeded species established in the study plots, but their response to herbicide rate showed few consistent patterns; some of the seeded species showed little response to herbicide, whereas others appeared to establish best at different herbicide rates, depending on site and whether the plots were burned or unburned. Site and burn treatment also affected how imazapic rate or application month influenced cover of perennial or annual grasses or forbs.
Synthesis and Properties of Amphiphilic Photoresponsive Gelators for Aromatic Solvents (2012)

A sugar-based photoresponsive supergelator, N-glycosylazobenzene that shows selective gelation of aromatic solvents is described. The partial trans-cis isomerization of the azobenzene moiety allows photoinduced chopping of the entangled gel fibers to short fibers, resulting in controlled fiber length and gel-sol transition. The gelator is useful for the selective removal of toxic aromatic solvents from water.
A blinded international study on the reliability of genetic testing for GGGGCC-repeat expansions in <i>C9orf72</i> reveals marked differences in results among 14 laboratories (2014)

Because of the wide range seen in genotyping results, we recommend using a combination of amplicon-length analysis and RP-PCR as a minimum in a research setting. We propose that Southern blotting techniques should be the gold standard, and be made obligatory in a clinical diagnostic setting.
Using the modified Delphi method to establish clinical consensus for the diagnosis and treatment of patients with rotator cuff pathology (2016)

This consensus guideline will help to standardize care, provide guidance on the diagnosis and treatment of rotator cuff pathology, and assist in clinical decision-making for all healthcare professionals.
An MILP Approach to Automated Wet-Etch Station Scheduling (2003)

Wet-etching in wafer fabrication is an automated process involving a complex interplay of mixed intermediate policies and material-handling constraints. Its operation poses a challenging resource-constrained flowshop scheduling problem that is crucial for enhancing productivity, improving yield, and minimizing wafer contamination. A novel continuous-time mixed-integer linear programming (MILP) formulation is presented for sequencing and scheduling wafer lots in an automated wet-etch station (AWS). Several reformulations and constraints are numerically evaluated to identify the best formulation. On the basis of this formulation, a near-optimum two-step strategy that is robust with respect to lot transfer times is developed for solving moderately sized problems.
Heat Extraction of Corn Fiber Hemicellulose (2007)

Water-soluble hemicellulose was extracted from corn fiber with microwave-assisted heat treatment. The effects of treatment temperature and initial pH of the aqueous extraction media were investigated regarding hemicellulose recovery and molecular mass of the isolated polysaccharides. In treatments carried out at neutral pH (simple water extraction), it has been demonstrated that hemicellulose recovery could be increased by applying higher treatment temperatures. However, the molecular weight of isolated hemicellulose gets significantly lower. For example, 10% of the raw materials’ xylan was extracted at 160°C and about 30% recovery was reached at 210°C. However, the molecular mass of the isolated polysaccharide at 210°C (5.82×104) was about half of that measured at 160°C (1.37×105). Reducing the pH with sulfuric acid resulted in shorter polymer chains (1.7×104) and lower hemicellulose yields (2.2%). Application of sodium hydroxide in the treatment showed that, compared with acid, considerably higher yields (11%) with longer polysaccharide chains (1.3×105) could be obtained.