gebhart/patrepeval huggingface dataset:
Comparison of the endotoxin-binding capacity of human transferrin and a human applicable immunoglobulin preparation. (1988)

Data obtained by in vitro experiments concerning the binding kinetics of the transferrin-endotoxin interactions are provided. The separation of protein-bound and free tritiated endotoxin of E. coli O 111:B4 is achieved by a simple precipitation method using ethanol. The main features of this reaction are the velocity of the saturation--saturation is reached after 2-3 min--and the requirement of divalent cations. EDTA (ethylenediaminetetraacetic acid) strongly inhibits the binding reaction of endotoxin to transferrin. The specificity of this interaction as well as the physiological importance can be demonstrated by the replacement of the radioactively labelled lipopolysaccharide preparation of E. coli 0 111:B4, by non-radioactive preparations of Salmonella minnesota, Serratia marcescens, E. coli 0 55:B5 and E. coli 0 111:B4. The described ethanol precipitation procedure for separating protein-bound and free endotoxin was applied to a commercially available immunoglobulin preparation. Although this preparation is known to exhibit high antibody titers against the lipid A moiety of lipopolysaccharides, no specific binding with the intact lipopolysaccharide can be found.
Antitumor Activity of Extracts from Medicinal Basidiomycetes Mushrooms (2016)

Aqueous extracts from the vegetative submerged mycelia of cultivated Basidiomycetes Ganoderma lucidum, Lentinus edodes, and Grifola frondosa, as well as from the fruiting bodies of G. lucidum, were found to have antitumor activity. The antitumor effect of the mycelial extracts from all 3 fungal species was ascertained in vivo in rats with implanted kidney cancer. Dystrophic changes in tumor cells and tumor necrosis (up to 90%) were noted. In vitro cytotoxicity studies of the A549 human lung adenocarcinoma cell line and HEp-2 human laryngeal epidermoid carcinoma cells showed that the extracts from the G. lucidum fruiting bodies and from the L. edodes vegetative mycelium were the most effective. The animals' immune systems were activated, and the fungal extracts displayed no toxicity when administered orally.
Long-term results and risk factor analysis for recurrence after curative endoscopic therapy in 349 patients with high-grade intraepithelial neoplasia and mucosal adenocarcinoma in Barrett’s oesophagus (2008)

This study showed that endoscopic therapy was highly effective and safe, with an excellent long-term survival rate. The risk factors identified may help stratify patients who are at risk for recurrence and those requiring more intensified follow-up.
Insulin Controls Subcellular Localization and Multisite Phosphorylation of the Phosphatidic Acid Phosphatase, Lipin 1 (2006)

Brain, liver, kidney, heart, and skeletal muscle from fatty liver dystrophy (fld/fld) mice, which do not express lipin 1 (lipin), contained much less Mg(2+)-dependent phosphatidic acid phosphatase (PAP) activity than tissues from wild type mice. Lipin harboring the fld(2j) (Gly(84) --> Arg) mutation exhibited relatively little PAP activity. These results indicate that lipin is a major PAP in vivo and that the loss of PAP activity contributes to the fld phenotype. PAP activity was readily detected in immune complexes of lipin from 3T3-L1 adipocytes, where the protein was found both as a microsomal form and a soluble, more highly phosphorylated, form. Fifteen phosphorylation sites were identified by mass spectrometric analyses. Insulin increased the phosphorylation of multiple sites and promoted a gel shift that was due in part to phosphorylation of Ser(106). In contrast, epinephrine and oleic acid promoted dephosphorylation of lipin. The PAP-specific activity of lipin was not affected by the hormones or by dephosphorylation of lipin with protein phosphatase 1. However, the ratio of soluble to microsomal lipin was markedly increased in response to insulin and decreased in response to epinephrine and oleic acid. The results suggest that insulin and epinephrine control lipin primarily by changing localization rather than intrinsic PAP activity.
Travel agents : Threatened intermediaries ? (1997)

In the past five years, direct electronic communication with consumers has become possible through the Internet, online services, and the use of telephones to communicate directly with computers. The case has been made that it will be possible to bypass intermediaries, as they will no longer be necessary for the coordination and management of information. As the role of intermediaries declines, profit margins along the value chain will be handed on to producers and consumers. Travel agents represent an important example of an intermediary between producers (such as airlines, hotels, or rental car firms) and consumers (prospective travelers). This article deals with the relationships between travel agents and those carriers, although agencies also represent other providers such as railroads, hotels, cruise lines, and rental car firms.
Pethidine as a spinal anaesthetic agent--a comparison with plain bupivacaine in patients undergoing transurethral resection of the prostate. (1992)

This study compares the anaesthetic effects of intrathecal pethidine or bupivacaine in patients undergoing surgery for transurethral resection of the prostate. Patients were randomly allocated to receive 3.5 ml of 0.5% plain bupivacaine or 1 mg kg-1 of 5% pethidine. The onset, extent and duration of sensory and motor blockade were studied. The cardiovascular and respiratory variables and frequency of side-effects were noted, both in the intra-operative period and the first 24 h of the post-operative period. There were no significant differences in intra-operative conditions or post-operative complications. The duration of block was significantly shorter with pethidine but was not associated with a difference in post-operative pain or analgesic requirements; this may have advantages when early post-operative mobility is indicated. We conclude that spinal pethidine is a satisfactory agent for transurethral resection of the prostate.
Liver Abscess Caused by Infection with Community-Acquired<i>Klebsiella quasipneumoniae</i>subsp.<i>quasipneumoniae</i> (2016)

We report a case of pyogenic liver abscess caused by community-acquired Klebsiella quasipneumoniae subsp. quasipneumoniae. The infecting isolate had 2 prominent features of hypervirulent K. pneumoniae strains: the capsular polysaccharide synthesis region for K1 serotype and the integrative and conjugative element ICEKp1, which encodes the virulence factors yersiniabactin, salmochelin, and RmpA.
Lipid and Lipoprotein Profiles in Youth With and Without Type 1 Diabetes (2008)

Youth with type 1 diabetes have abnormal lipid levels and atherogenic changes in lipoprotein composition, even after a relatively short disease duration. As in adults, glycemic control is an important mediator of these abnormalities.
Intestinal Colonization by a <i>Lachnospiraceae</i> Bacterium Contributes to the Development of Diabetes in Obese Mice (2014)

The aim of the present study was to identify bacteria that may contribute to the onset of metabolic dysfunctions. We isolated and identified a candidate bacterium belonging to Lachnospiraceae (strain AJ110941) in the feces of hyperglycemic obese mice. The colonization of germ-free ob/ob mice by AJ110941 induced significant increases in fasting blood glucose levels as well as liver and mesenteric adipose tissue weights, and decreases in plasma insulin levels and HOMA-β values. These results indicated that the specific gut commensal bacterium AJ110941 influenced the development of obesity and diabetes in ob/ob mice with genetic susceptibility for obesity.
A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics (2011)

The fast-growing biopharmaceutical industry demands speedy development of highly efficient and reliable production systems to meet the increasing requirement for drug supplies. The generation of production cell lines has traditionally involved manual operations that are labor-intensive, low-throughput and vulnerable to human errors. We report here an integrated high-throughput and automated platform for development of manufacturing cell lines for the production of protein therapeutics. The combination of BD FACS Aria Cell Sorter, CloneSelect Imager and TECAN Freedom EVO liquid handling system has enabled a high-throughput and more efficient cell line development process. In this operation, production host cells are first transfected with an expression vector carrying the gene of interest (1), followed by the treatment with a selection agent. The stably-transfected cells are then stained with fluorescence-labeled anti-human IgG antibody, and are subsequently subject to flow cytometry analysis (2-4). Highly productive cells are selected based on fluorescence intensity and are isolated by single-cell sorting on a BD FACSAria. Colony formation from single-cell stage was detected microscopically and a series of time-laps digital images are taken by CloneSelect Imager for the documentation of cell line history. After single clones have formed, these clones were screened for productivity by ELISA performed on a TECAN Freedom EVO liquid handling system. Approximately 2,000 - 10,000 clones can be screened per operation cycle with the current system setup. This integrated approach has been used to generate high producing Chinese hamster ovary (CHO) cell lines for the production of therapeutic monoclonal antibody (mAb) as well as their fusion proteins. With the aid of different types of detecting probes, the method can be used for developing other protein therapeutics or be applied to other production host systems. Comparing to the traditional manual procedure, this automated platform demonstrated advantages of significantly increased capacity, ensured clonality, traceability in cell line history with electronic documentation and much reduced opportunity in operator error.
Supporting collaboration and multiple views of building models in virtual worlds (2005)

Collaboration in virtual environments has the potential to support communication and collaboration in multi-disciplinary teams. Current software for documenting and developing models of buildings focuses on supporting a single user who is a specialist in the specific software used within their own discipline. In this paper we report on the research, and a proposed approach, in supporting multiple disciplines in virtual worlds. We emphasise that the views and models are founded on the functional concerns of designers and the concerns are discipline specific. An agentbased approach to representing multiple views is described, along with a virtual world environment that supports communication and collaboration. In order to prototype and develop a collaborative environment, we consider simple models in an inherently multi-user virtual world.
Parallel canonical recoding (1996)

The author introduces a parallel algorithm for generating the canonical signed-digit expansion of an n-bit number in O(logn) time using O(n) gates. The algorithm is similar to the computation of the carries in a carry look-ahead circuit. It is also proven that if the binary number x + ⌋x/2⌊ is given, then the canonical signed-digit recoding of x can be computed in O(1) time using O(n) gates.
Defining the target specificity of ABT-737 and synergistic antitumor activities in combination with histone deacetylase inhibitors (2008)

The apoptotic and therapeutic activities of the histone deacetylase inhibitor (HDACi) vorinostat are blocked by overexpression of Bcl-2 or Bcl-X(L). Herein, we used the small molecule inhibitor ABT-737 to restore sensitivity of Emu-myc lymphomas overexpressing Bcl-2 or Bcl-X(L) to vorinostat and valproic acid (VPA). Combining low-dose ABT-737 with vorinostat or VPA resulted in synergistic apoptosis of these cells. ABT-737 was ineffective against Emu-myc/Mcl-1 and Emu-myc/A1 cells either as a single agent or in combination with HDACi. However, in contrast to the reported binding specificity data, Emu-myc/Bcl-w lymphomas were insensitive to ABT-737 used alone or in combination with HDACi, indicating that the regulatory activity of ABT-737 is restricted to Bcl-2 and Bcl-X(L). Emu-myc lymphomas that expressed Bcl-2 throughout the tumorigenesis process were especially sensitive to ABT-737, while those forced to overexpress Mcl-1 were not. This supports the notion that tumor cells "addicted" to ABT-737 target proteins (ie, Bcl-2 or Bcl-X(L)) are likely to be the most sensitive target cell population. Our studies provide important preclinical data on the binding specificity of ABT-737 and its usefulness against primary hematologic malignancies when used as a single agent and in combination with HDACi.
An Investigation of Different Molting Techniques with an Emphasis on Animal Welfare (2002)

We experimented to evaluate the effect of a number of molting techniques that appeared to be less stressful than the conventional feed withdrawal (FW) method on postmolt performance. The molting techniques involved a continuous FW (T1, control group), 1 d FWfollowed by feeding a grape pomace (GP) diet containing 10 ppm thyroxine (T2) ad libitum, feeding a corn diet (T3) ad libitum, or the corn diet with an initial FW of 1 d (T4) or 2 d (T5), or regimens similar to T3, T4, and T5, respectively, in which the corn diet contained 10 ppm thyroxine (T6, T7, and T8). Induction of molting started at 66 wk of age and lasted until 30% body weight reduction (BWR) or 28 d—whichever came first. Postmolt performance information was collected up to 98 wk of age. The 30% BWR was obtained after 14 d FWwith T1 and after 16 d FWwith T2. Hens exposed to T6, T7, and T8 had a BWR of 26 to 29% after 28 d, and those exposed to T3, T4, and T5 had a BWR of 16 to 18% after 28 d from the start of induction of molt. Postmolt egg production (EP) was consistently greater for T1 and T2 than the other molting techniques. Other production traits, egg quality, and serum corticosterone, for the most part, were not different among various molting techniques. The results indicated that use of a GP diet plus thyroxine could support a similar postmolt performance as the conventional method of continuous FW. Unfortunately, the physiological response did not indicate that the use of a GP diet plus thyroxine was less stressful than the conventional method of continuous FW. Nevertheless, because use of the GP diet plus thyroxin supplies the hens continuously with some nutrients such as energy, protein, vitamins and minerals, etc., during induction of molt, this approach seems preferable due to ever-increasing public concern surrounding the hen welfare and the long duration of FW in a conventional FW technique.
Deposition of Conducting Polyaniline Patterns with the Scanning Electrochemical Microscope (1997)

Conducting polyaniline patterns were deposited on gold, platinum, and carbon surfaces with the use of the scanning electrochemical microscope (SECM). The patterns were deposited in the "microreagent" mode in which a local pH change caused by proton reduction at the SECM tip allowed deposition to occur at the substrate. The effect of tip and substrate potential, tip‐substrate separation, and deposition time were studied in order to produce well‐resolved patterns of the desired thickness. Lateral resolution of the deposited polymer was as low as 3 μm. Conductivity of the film was verified by SECM imaging.
Automatic Music Mood Detection through Musical Structure Analysis (2009)

Since music has a close relation with people emotion, many researchers have studied on detecting music mood. Usually, they manually selected the representative segment of music and classified its mood. Although such manual approaches correctly detect the mood, their manual intervention makes their application for new music difficult. Moreover, the mood variation in music makes their application more difficult. To cope with these problems, we present an automatic method to classify the music mood. After the music is segmented into groups having similar characteristics by structural information, the support vector regressors are constructed with musical features from pitch, beat, and spectrum of each segment. The mood of unknown music is predicted by the regressors. Experimental results show that performance of the proposed approach is comparable to the performances of manual approaches.
Polymorphisms of IL-1 beta gene in Japanese patients with Sjögren's syndrome and systemic lupus erythematosus. (2004)

IL-1 beta gene polymorphisms may affect susceptibility to SS, but not SLE.
(Ba,Sr)TiO 3 thin films with conducting perovskite electrodes for dynamic random access memory applications (1999)

Interfaces and hence electrodes determine the performance of (Ba,Sr)TiO3 (BST) capacitors for ultralarge scale integration dynamic random access memories. Electrode materials forming a rectifying contact on BST drastically reduce the dielectric constant and hence the capacitance and charge storage density of the capacitor, when the dielectric thickness is reduced. This can limit the role of Pt as an electrode material for gigabit dynamic random access memories (DRAM). The conducting oxide, La0.5Sr0.5CoO3 (LSCO) with its perovskite structure, has structural and chemical compatibility with BST. Our results in LSCO/BST/LSCO capacitor show that the mechanism of conduction is not interface limited but predominantly bulk limited. A 75 nm BST film with LSCO electrodes shows a leakage current density of 1×10−7 A/cm2 at 1 V, 85 °C. The dielectric constant at 1 V, 105 Hz is 350, making LSCO a potential contact electrode for DRAM memories.
Birefringence and Crystallization in Elastomers (1955)

The semitheoretical expression, fc=Δn0−csFΔnc0−cs,where fc=volume fraction crystalline, Δn0=observed birefringence, c=the stress-optical coefficient, s=the stress on actual cross section, F=a known function of crystallite orientation, and Δnc0=the birefringence of a single polymer crystal, is proposed for relating birefringence, stress, and degree of crystallinity in polycrystalline elastomers under tensile stress. This expression is shown to yield results in substantial agreement with x-ray results for the degree of crystallinity of 5°C polybutadiene when Δnc0=0.157. Birefringencedensity data on natural rubber (due to L. R. G. Treloar) are shown to be a special case of the foregoing expression, yielding a value Δnc0=0.218 for natural rubber which is in good agreement with the theoretical value for this quantity.
Walk and talk (2016)

Use these activities linked to the popular picture book Rosie's Walk to help children explore the use of preposition words and practise using them. It all starts with a walk around a farm..
Reversibly switchable polymer with cationic/zwitterionic/anionic behavior through synergistic protonation and deprotonation (2013)

A polymer capable of fully and reversibly switching throughout the entire charge regime is highly desirable for many applications such as drug and gene delivery, controlled ion and molecular transport and tunable filtration membranes. It is essential that for biologically relevant applications the polymer needs to be nonfouling. However, conventional nonfouling zwitterionic polymers have a permanently positive quaternary nitrogen center, making it impossible to switch between charges. Here, we present a rationally designed polymer with a tertiary amine and a carboxylic acid, which is capable of reversibly switching among three distinct charged states, viz., cationic, zwitterionic and anionic, and importantly maintaining the zwitterionic state under physiological pH conditions. Oppositely charged proteins adsorbed on a charged surface selectively can be completely removed by switching the surface to the zwitterionic state. We have also found that these two moieties (i.e., a tertiary amine and a carboxylate moiety) stimulate each other synergistically to achieve a strongly zwitterionic state under physiological conditions and to resist non-specific protein adsorption from undiluted blood plasma and serum when they are close to each other.
Compounds Affecting the Central Nervous System. 4. 3.beta.-Phenyltropane-2-carboxylic Esters and Analogs (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCompounds Affecting the Central Nervous System. 4. 3.beta.-Phenyltropane-2-carboxylic Esters and AnalogsRobert Clarke, Sol Daum, Anthony Gambino, Mario Aceto, Jack Pearl, Morton Levitt, Wayne Cuminskey, and Eugenio BogadoCite this: J. Med. Chem. 1973, 16, 11, 1260–1267Publication Date (Print):November 1, 1973Publication History Published online20 May 2003Published inissue 1 November 1973https://pubs.acs.org/doi/10.1021/jm00269a600https://doi.org/10.1021/jm00269a600research-articleACS PublicationsRequest reuse permissionsArticle Views751Altmetric-Citations198LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Developing an Augmented Reality Racing Game (2008)

Augmented reality (AR) makes it possible to create games in which virtual objects are overlaid on the real world, and real objects are tracked and used to control virtual ones. We describe the development of an AR racing game created by modifying an existing racing game, using an AR infrastructure t