gebhart/patrepeval huggingface dataset:
A consumer participant's perspective of the inaugural consumers reforming health conference 18-20 July 2011 (2011)

Representing Central Bayside Community Health Services, I shared the conference with Shirley Hynes, the other consumer representative on the Consumer, Carer and Community Participation committee. She attended Monday and I went Tuesday and Wednesday. The conference area at the Melbourne Exhibition and Convention Centre buzzed with activity as I collected a bag of information including the 85-page program with presenters' bios and abstracts. I immediately thought what an ambitious program of high quantity and quality - but perhaps too much for three days! Like many others, I had difficulty making a choice between the concurrent sessions but chose The Case For Consumer Participation and Learning From Research Involving Consumers. Living with breast cancer, I'm involved with Lifepool and with two daughters the continuing of research is important to me, and consumer involvement must not just be about securing funding.
Osteoclast differentiation and activity (1998)

Conference Article| February 01 1998 Osteoclast differentiation and activity G. D. Roodman G. D. Roodman 1The Audie Murphy V. A. Hospital and the Department of Medicine, Division of Hematology, University of Texas Health Science Center, San Antonio, TX 78284, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1998) 26 (1): 7–13. https://doi.org/10.1042/bst0260007 Article history Received: July 21 1997 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation G. D. Roodman; Osteoclast differentiation and activity. Biochem Soc Trans 1 February 1998; 26 (1): 7–13. doi: https://doi.org/10.1042/bst0260007 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: CFU-GM, granulocyte-macrophage progenitor cell, M-CSF, macrophage colony stimulating factor, GM-CSF, granulocyte-macrophage colony stimulating factor, PTH, parathyroid hormone, PTHrP, parathyroid hormone related protein, TNF, tumour necrosis factor, TGFβ, transforming growth factor-beta, γ-IFN, gamma interferon, OIP, osteoclast inhibitory peptide, IL, interleukin This content is only available as a PDF. © 1998 Biochemical Society1998 Article PDF first page preview Close Modal You do not currently have access to this content.
Advances in Computation of the Maximum of a Set of Random Variables (2006)

This paper quantifies the approximation error in Clark's approach presented in C. E. Clark (1961) to computing the maximum (max) of Gaussian random variables; a fundamental operation in statistical timing. We show that a finite look up table can be used to store these errors. Based on the error computations, approaches to different orderings for pair-wise max operations on a set of Gaussians are proposed. Experiments show accuracy improvements in the computation of the max of multiple Gaussians by up to 50% in comparison to the traditional approach. To the best of our knowledge, this is the first work addressing the mentioned issues.
Characterization of four nucleic acid-binding single-chain Fv fragments by direct and competitive solid-phase radioimmunoassays (1994)

The heavy- and light-chain variable region genes of four different nucleic acid-binding antibodies (Jel 274 and Jel 72 (specific for duplex DNA), Jel 103 (specific for poly(rI)), and Jel 318 (specific for DNA triplexes)) were cloned. Single-chain Fv fragments (scFv) in which the heavy and light chains were joined by a linker were constructed by polymerase chain reaction. The linker of 21 amino acids also served as a tag since it consisted of a repeating heptapeptide that was recognized by a specific antipeptide antibody. scFv were expressed in the cytoplasm of Escherichia coli as inclusion bodies. After purification and renaturation, a cross-linking assay was used to demonstrate that > 90% of scFv were in the form of monomers. The specificity of scFv was analyzed by both direct and competitive solid-phase radioimmunoassays. scFv.103 retained its specificity for poly(rI), whereas the other three scFv still bound the original antigen, but subtle changes in the overall specificity were noted. Thus, in some cases, the conformation of the binding site may be different in the context of an scFv compared with the original IgG.
Formation of Ni silicide from Ni(Au) films on (111)Si (1996)

The solid state reaction between a Ni (7 at. % Au) film and a Si substrate at temperatures ranging from 250 to 800 °C is examined by scanning electron microscopy, x-ray diffraction, and Rutherford backscattering spectrometry. Compared to the usual features for thin film reaction of Ni with Si, we observed the following. (i) The simultaneous growth of Ni2Si and NiSi, and the growth of NiSi at the expense of both Ni2Si and Ni. This is related to Au accumulation in the metal layer. (ii) Au precipitation at 300 °C followed by the dissolution of the clusters thus created above the Au–Si eutectic temperature (370 °C). (iii) A decrease of the temperature of formation of NiSi2 and the appearance of thickness oscillations that are characteristic of nucleation. These different effects are interpreted by taking into account the metallurgy of the system: segregation of Au in the Ni film, Au solubility in the different silicides, change in surface and interface energies, and chemical interactions with Si.
NetQuery (2011)

This paper presents the design and implementation of NetQuery, a knowledge plane for federated networks such as the Internet. In such networks, not all administrative domains will generate information that an application can trust and many administrative domains may have restrictive policies on disclosing network information. Thus, both the trustworthiness and accessibility of network information pose obstacles to effective reasoning. NetQuery employs trustworthy computing techniques to facilitate reasoning about the trustworthiness of information contained in the knowledge plane while preserving confidentiality guarantees for operator data. By characterizing information disclosure between operators, NetQuery enables remote verification of advertised claims and contractual stipulations; this enables new applications because network guarantees can span administrative boundaries. We have implemented NetQuery, built several NetQuery-enabled devices, and deployed applications for cloud datacenters, enterprise networks, and the Internet. Simulations, testbed experiments, and a deployment on a departmental network indicate NetQuery can support hundreds of thousands of operations per second and can thus scale to large ISPs.
Joint Collaborative Representation With Multitask Learning for Hyperspectral Image Classification (2014)

In this paper, we propose a joint collaborative representation (CR) classification method with multitask learning for hyperspectral imagery. The proposed approach consists of the following aspects. First, several complementary features are extracted from the hyperspectral image. We next apply these features into the unified multitask-learning-based CR framework to acquire a representation vector and adaptive weight for each feature. Finally, the contextual neighborhood information of the image is incorporated into each feature to further improve the classification performance. The experimental results suggest that the proposed algorithm obtains a competitive performance and outperforms other state-of-the-art regression-based classifiers and the classical support vector machine classifier.
Comprehensive Structure–Activity Relationship of Triantennary<i>N</i>-Acetylgalactosamine Conjugated Antisense Oligonucleotides for Targeted Delivery to Hepatocytes (2016)

The comprehensive structure-activity relationships of triantennary GalNAc conjugated ASOs for enhancing potency via ASGR mediated delivery to hepatocytes is reported. Seventeen GalNAc clusters were assembled from six distinct scaffolds and attached to ASOs. The resulting ASO conjugates were evaluated in ASGR binding assays, in primary hepatocytes, and in mice. Five structurally distinct GalNAc clusters were chosen for more extensive evaluation using ASOs targeting SRB-1, A1AT, FXI, TTR, and ApoC III mRNAs. GalNAc-ASO conjugates exhibited excellent potencies (ED50 0.5-2 mg/kg) for reducing the targeted mRNAs and proteins. This work culminated in the identification of a simplified tris-based GalNAc cluster (THA-GN3), which can be efficiently assembled using readily available starting materials and conjugated to ASOs using a solution phase conjugation strategy. GalNAc-ASO conjugates thus represent a viable approach for enhancing potency of ASO drugs in the clinic without adding significant complexity or cost to existing protocols for manufacturing oligonucleotide drugs.
Cloth animation with adaptively refined meshes (2005)

Cloth animation is a very expensive process in terms of computational cost, due to the flexible nature of cloth objects. Since wrinkles and smooth areas co-exist commonly in cloth, it is tempting to reduce computational cost by avoiding redundant tessellation at the smooth areas. In this paper we present a method for dynamic adaptation of triangular meshes suitable for cloth simulation. A bottom-up approach is used for mesh refinement, which does not require precomputation and storage of multiresolution hierarchy. The hierarchy is constructed in runtime and allows reverting of the refinement locally. Local mesh refinement and simplification are triggered by curvature-induced criterion, where the curvature is estimated using methods of discrete differential geometry. The results presented are the realistic animation of garment worn by a walking mannequin generated with Baraff-Witkin type cloth solver enhanced with the mesh adaptation scheme.
Development and demonstration of a precision latch for deployable optical systems (2003)

The Lockheed Martin/Advanced Technology Center (LM/ATC) developed a lightweight, compact, high-load capable and yet high precision latch for use on deployable optical systems such as the Next Generation Space Telescope (NGST). The design allows precise self-centering and control of the stiffness at the latch interface. It also incorporates unique capabilities to evaluate the effects of gravity loads, latch preload level, creep, and very low vibration loads on the dynamics and microdynamics of the deployed instrument. The stiffness, nonlinearity and hysteresis characteristics of the latch and its catch flexure assembly were thoroughly tested in 6 axes down to the nanometer level at room temperature using the LM/ATC Compliance Measurement Device. The latch is stiff enough to hold an NGST-size mirror segment cantilevered against gravity allowing only small gravity sag when the primary mirror is horizontal, thus enabling end-to-end performance verification in 1-G in that orientation. The latch hysteresis is less than 1.0 nm/N under mechanical loads less than 25 N, which meets the NGST stability requirements with significant margin (20 nm at the tip of the petal in space environment). Several of these latches were integrated and demonstrated at the petal assembly level on a Single Petal Test-bed and the experimental results obtained on that test-bed are consistent with the component level results described in this report. We experimentally demonstrated that the latch engagement performance is not affected by exposure to cryogenic temperatures down to 20K, as required for use of the device on cryogenic infrared optical instruments such as NGST. A structural model of the latch was developed using Finite Element Analysis. Good correlation was obtained between the linear components of the analytical and of the experimental results: the model can therefore reliably be used in future NGST or other mission design efforts. This paper includes a brief description of the LM/ATC latch hardware and its principle of operation as well as the results of the modeling and the experimental characterization work performed on that hardware in the NGST Phase I formulation.
CD34 and CD49f Double-Positive and Lineage Marker-Negative Cells Isolated from Human Myometrium Exhibit Stem Cell-Like Properties Involved in Pregnancy-Induced Uterine Remodeling1 (2015)

Repeated and dramatic pregnancy-induced uterine enlargement and remodeling throughout reproductive life suggests the existence of uterine smooth muscle stem/progenitor cells. The aim of this study was to isolate and characterize stem/progenitor-like cells from human myometrium through identification of specific surface markers. We here identify CD49f and CD34 as markers to permit selection of the stem/progenitor cell-like population from human myometrium and show that human CD45(-) CD31(-) glycophorin A(-) and CD49f(+) CD34(+) myometrial cells exhibit stem cell-like properties. These include side population phenotypes, an undifferentiated status, high colony-forming ability, multilineage differentiation into smooth muscle cells, osteoblasts, adipocytes, and chondrocytes, and in vivo myometrial tissue reconstitution following xenotransplantation. Furthermore, CD45(-) CD31(-) glycophorin A(-) and CD49f(+) CD34(+) myometrial cells proliferate under hypoxic conditions in vitro and, compared with the untreated nonpregnant myometrium, show greater expansion in the estrogen-treated nonpregnant myometrium and further in the pregnant myometrium in mice upon xenotransplantation. These results suggest that the newly identified myometrial stem/progenitor-like cells influenced by hypoxia and sex steroids may participate in pregnancy-induced uterine enlargement and remodeling, providing novel insights into human myometrial physiology.
A Hybrid Approach to Detect and Localize Texts in Natural Scene Images (2010)

Text detection and localization in natural scene images is important for content-based image analysis. This problem is challenging due to the complex background, the non-uniform illumination, the variations of text font, size and line orientation. In this paper, we present a hybrid approach to robustly detect and localize texts in natural scene images. A text region detector is designed to estimate the text existing confidence and scale information in image pyramid, which help segment candidate text components by local binarization. To efficiently filter out the non-text components, a conditional random field (CRF) model considering unary component properties and binary contextual component relationships with supervised parameter learning is proposed. Finally, text components are grouped into text lines/words with a learning-based energy minimization method. Since all the three stages are learning-based, there are very few parameters requiring manual tuning. Experimental results evaluated on the ICDAR 2005 competition dataset show that our approach yields higher precision and recall performance compared with state-of-the-art methods. We also evaluated our approach on a multilingual image dataset with promising results.
Validity, reliability, and feasibility of durometer measurements of scleroderma skin disease in a multicenter treatment trial (2008)

Durometer measurements of skin hardness in patients with scleroderma are reliable, simple, accurate, demonstrate good sensitivity to change compared with traditional skin scoring, and reflect patients' self-assessments of their disease. Durometer measurements are valid, objective, and scalable, and should be considered for use as a complementary outcome measure to skin scoring in clinical trials of scleroderma.
The DNA-bending protein HMGB1 is a cellular cofactor of Sleeping Beauty transposition (2003)

Sleeping Beauty (SB) is the most active Tc1/ mariner-type transposon in vertebrates. SB contains two transposase-binding sites (DRs) at the end of each terminal inverted repeat (IR), a feature termed the IR/DR structure. We investigated the involvement of cellular proteins in the regulation of SB transposition. Here, we establish that the DNA-bending, high-mobility group protein, HMGB1 is a host-encoded cofactor of SB transposition. Transposition was severely reduced in mouse cells deficient in HMGB1. This effect was rescued by transient over-expression of HMGB1, and was partially complemented by HMGB2, but not with the HMGA1 protein. Over-expression of HMGB1 in wild-type mouse cells enhanced transposition, indicating that HMGB1 can be a limiting factor of transposition. SB transposase was found to interact with HMGB1 in vivo, suggesting that the transposase may recruit HMGB1 to transposon DNA. HMGB1 stimulated preferential binding of the transposase to the DR further from the cleavage site, and promoted bending of DNA fragments containing the transposon IR. We propose that the role of HMGB1 is to ensure that transposase-transposon complexes are first formed at the internal DRs, and subsequently to promote juxtaposition of functional sites in transposon DNA, thereby assisting the formation of synaptic complexes.
Efficient Enzymatic Process for the Production of (2<i>S</i>)-4,4-Difluoro-3,3-dimethyl-<i>N</i>-Boc-proline, a Key Intermediate in the Synthesis of HIV Protease Inhibitors (2006)

(2S)-4,4-Difluoro-3,3-dimethyl-N-Boc-proline (3) is a key intermediate for the synthesis of HIV protease inhibitors. Here, several approaches for the preparation of enantiopure 3 and its analogues are disclosed. Among these methods, one strategy relies on resolving the racemic methyl ester of 3 through a protease-catalyzed enantioselective hydrolysis. Despite the fact that this resolution was applied to prepare kilogram quantities of optically pure acid 3 for clinical trials, this process suffered from low efficiency, high cost and difficulties in improvement by medium engineering. An alternative much more efficient and cost-effective enzymatic process was therefore developed by switching the protective group of the proline esters from a Boc to a benzyl moiety. This new process has a much higher throughput (6.3 mmol/h/L vs 0.11 mmol/h/L), and the cost of the process was also dramatically reduced to only 5% of the protease resolution process.
Reversed phase ion-pairing HPLC-ICP-MS for analysis of organophosphorus chemical warfare agent degradation products (2006)

The separation and analysis of three organophosphorus chemical warfare degradation products is described. Ethyl methylphosphonic acid (EMPA, the major hydrolysis product of VX), isopropyl methylphosphonic acid (IMPA, the major hydrolysis product of Sarin (GB)), and methylphosphonic acid (MPA, the final hydrolysis product of both) were the analytes and were separated by reversed phase ion-pairing high-performance liquid chromatography (RP-IP-HPLC) with the use of myristyl trimethylammonium bromide as ion-pairing reagent and an ammonium acetate–acetic acid buffer system (pH 4.85). An Agilent 7500ce inductively coupled plasma mass spectrometer (ICP-MS) equipped with collision/reaction cell technology was coupled to the chromatographic system for detection of 31P and 47PO+. Historically, ICP-MS detection of phosphorus has been limited due to its high first ionization potential (10.5 eV) and the presence of severe nitrogen polyatomic interferences (such as 14N16O1H+ and 15N16O+) overlapping its only isotope at m/z = 31. Implementation of an octopole reaction cell with helium as the cell gas allowed for removal of the nitrogen polyatomic interferences and reduction of background signal. Detection limits for EMPA, IMPA, and MPA were found to be 263, 183 and 139 pg mL−1, respectively, with separation in less than 15 min. The developed method was successfully applied to the analysis of spiked environmental water and soil samples.
Quantitative two-dimensional echocardiographic measurements are major predictors of adverse cardiovascular events after acute myocardial infarction. The protective effects of captopril. (1994)

Two-dimensional echocardiography provides important and independent prognostic information in patients after infarction. Left ventricular enlargement and function after infarction are associated with the development of adverse cardiac events. Attenuation of ventricular enlargement with captopril in these patients was associated with a reduction in adverse events. This study demonstrates the linkage between attenuation of left ventricular enlargement by captopril after infarction and improved clinical outcome.
Potential 129Xe-NMR biosensors based on secondary and tertiary complexes of a water-soluble pillar[5]arene derivative (2013)

We report on the first secondary and tertiary complexes of the pillar[5]arene derivative with xenon in water. We show that the chemical shift of the encapsulated xenon provides information on the type of the formed complex suggesting that has the potential to be used as a platform for NMR biosensors.
Locally excitatory globally inhibitory oscillator networks: theory and application to pattern segmentation (2002)

An novel class of locally excitatory, globally inhibitory oscillator networks (LEGION) is proposed and investigated analytically and by computer simulation. The model of each oscillator corresponds to a standard relaxation oscillator with two time scales. The network exhibits a mechanism of selective gating, whereby an oscillator jumping up to its active phase rapidly recruits the oscillators stimulated by the same pattern, while preventing other oscillators from jumping up. We show analytically that with the selective gating mechanism the network rapidly achieves both synchronization within blocks of oscillators that are stimulated by connected regions and desynchronization between different blocks. Computer simulations demonstrate LEGION's promising ability for segmenting multiple input patterns in real time. This model lays a physical foundation for the oscillatory correlation theory of feature binding, and may provide an effective computational framework for pattern segmentation and figure/ground segregation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Probabilistic Top-Down Parsing and Language Modeling (2001)

This paper describes the functioning of a broad-coverage probabilistic top-down parser, and its application to the problem of language modeling for speech recognition. The paper first introduces key notions in language modeling and probabilistic parsing, and briefly reviews some previous approaches to using syntactic structure for language modeling. A lexicalized probabilistic top-down parser is then presented, which performs very well, in terms of both the accuracy of returned parses and the efficiency with which they are found, relative to the best broad-coverage statistical parsers. A new language model that utilizes probabilistic top-down parsing is then outlined, and empirical results show that it improves upon previous work in test corpus perplexity. Interpolation with a trigram model yields an exceptional improvement relative to the improvement observed by other models, demonstrating the degree to which the information captured by our parsing model is orthogonal to that captured by a trigram model. A small recognition experiment also demonstrates the utility of the model.