gebhart/patrepeval huggingface dataset:
The penicillin regulator PENR1 of <i>Aspergillus nidulans</i> is a HAP‐like transcriptional complex (1998)

In Aspergillus nidulans, a DNA-binding complex, PENR1, was shown to bind to two CCAAT-box-containing DNA elements located in the promoter regions of the bidirectionally oriented penicillin biosynthesis genes acvA and ipnA, and of the aat promoter. Here, partial purification of PENR1 and western blotting using anti-HAPC sera indicated that the previously identified HAPC protein, which was suggested to be part of the CCAAT-binding complex AnCF, is also part of PENR1. This was confirmed by band shift assays using protein extracts of a delta hapC strain which exhibited no PENR1 DNA-binding activity. Supershift assays and immunoprecipitation analysis using anti-HAPC sera provided evidence that HAPC is part of the PENR1 complex. In delta hapC strains, penicillin production was reduced, as was expression of both an ipnA-lacZ and aat-lacZ gene fusion. Hence, HAPC-containing PENR1 appears to act as an activator on ipnA and aat expression. However, deletion of hapC had little effect on acvA expression during a fermentation run in fermentation medium. Previous results which had shown that specific deletion of the PENR1-binding site between acvA and ipnA resulted in a strong increase of expression of an acvA-uidA gene fusion, together with the present data, suggest the possibility of the existence of a repressor protein that binds close to or overlaps the PENR1-binding site. It is also shown that binding of PENR1 induced bending of a DNA fragment spanning the PENR1-binding site between acvA and ipnA in vitro.
Effects of Various Iron Fortificants on Sensory Acceptability and Shelf-Life Stability of Instant Noodles (2007)

Iron fortification of wheat flour used to make instant noodles is feasible. NaFeEDTA is the preferred fortificant because of its nonsignificant effect on the color and sensory quality of the products.
Effects of Single Oral Intake of Kaempferia parviflora Extract on Energy Metabolism―A Randomized Double—blind Crossover Study― (2016)

Objective The present study examined the effects of Kaempferia parviflora extract （KPE） supplementation on energy metabolism and fat utilization rate in healthy subjects. Methods A randomized double-blind crossover study was conducted to assess the effect of a single oral intake of KPE on 15 healthy volunteers. Respiratory quotient（RQ）and parameters of heart rate variability were determined during constant low-intensity exercise（30％ of maximal oxygen consumption）after intake of the test food. Results After excluding outliers, the number of subjects analyzed was twelve. There were no significant differences in any of the parameters of heart rate variability between the placebo and KPE groups. Single oral intake of KPE significantly attenuated the increase of RQ by exercise compared to the placebo. Conclusion Our data suggest that a single oral intake of KPE increases the rate of fat utilization during exercise at a constant intensity, and is a potential tool for the prevention and improvement of obesity and metabolic syndrome.
New Insights Regarding the Autoxidation of Polyunsaturated Fatty Acids (2004)

Free radical-initiated autoxidation of polyunsaturated fatty acids (PUFAs) has been implicated in numerous human diseases, including atherosclerosis and cancer. This review covers the free radical mechanisms of lipid oxidation and recent developments of analytical techniques to analyze the lipid oxidation products. Autoxidation of PUFAs generates hydroperoxides as primary oxidation products, and further oxidation leads to cyclic peroxides as secondary oxidation products. Characterization of these oxidation products is accomplished by several mass spectrometric techniques. Ag+ coordination ion spray mass spectrometry has proven to be a powerful tool to analyze the intact lipid peroxides. Monocyclic peroxides, bicyclic endoperoxides, serial cyclic peroxides, and a novel class of endoperoxides (dioxolane-isoprostane peroxides) have been identified from the oxidation of arachidonate. Electron capture atmospheric pressure chemical ionization mass spectrometry has been applied to study lipid oxidation products after derivatization. All eight possible diastereomeric isoprostanes are observed from the oxidation of a single hydroperoxide precursor. 5- and 15-series isoprostanes are more abundant than the 8- and 12-series because the precursors that lead to 8- and 12-series compounds can undergo further oxidation and form dioxolane-isoprostane peroxides. Furthermore, formation of isoprostanes from 15-hydroperoxyeicosatetraenoate occurs from beta-fragmentation of the corresponding peroxyl radical to generate a pentadienyl radical rather than a "dioxetane" intermediate, as previously suggested.
Polynucleotide Sequence Relatedness Among Three Groups of Pathogenic <i>Escherichia coli</i> Strains (1972)

Escherichia coli strains that cause dysentery-like disease, parenteral infection, and infantile diarrhea form specific groups based on mobility of O and K antigens in immunoelectrophoresis. Members from each of these groups were assayed for gross nucleotide sequence relatedness. The method used was interspecific deoxyribonucleic acid (DNA) reassociation reactions carried out free in solution. Reassociated DNA was separated from unreacted DNA by passage through hydroxyapatite. DNA relatedness between these groups was approximately 80%. The groups containing those strains causing parenteral infection and those responsible for dysentery-like disease showed preferentially high intragroup DNA relatedness. The group containing strains responsible for infantile diarrhea did not show preferentially high intragroup DNA relatedness with the reference strain employed. These strains, however, did exhibit preferentially high DNA relatedness to a second reference strain.
Fexapotide triflutate: results of long-term safety and efficacy trials of a novel injectable therapy for symptomatic prostate enlargement (2018)

FT 2.5 mg is a safe and effective transrectal injectable for LT treatment of BPH. FT treated patients also had reduced need for BPH intervention, and reduced incidence of PCa and AUR.
The sufficient minimal set of miRNA seed types (2011)

We identified a set of canonical seed types based on a transcriptome wide analysis of experimentally verified functional target sites. We confirmed the specificity of long seeds but we found that the majority of functional target sites are formed by less specific seeds of only 6 nt indicating a crucial role of this type. A substantial fraction of genuine target sites arenon-conserved. Moreover, the majority of functional sites remain uncovered by common prediction methods.
Multiple mycobacterial antigens are targets of the adaptive immune response in pulmonary sarcoidosis (2010)

Together these results reveal that antigen-specific CD4+ and CD8+ T cells responses to multiple mycobacterial epitopes are present within sites of active sarcoidosis involvement, and that these antigen-specific responses are present at the time of diagnosis.
A Comparative Analysis of Enterprise Architecture Frameworks Based on EA Quality Attributes (2009)

Many Enterprise Architecture Frameworks (EAFs) are in use today to guide or serve as a model for the enterprise architecture development. Researchers offer general comparative information about EAF. However, there has been little work on the quality of Enterprise Architecture (EA). This study provides the characteristics of the five EAFs using comparative analysis based on Enterprise Architecture Quality Attributes (EAQAs). The attribute of EA quality was extracted from the EAFs and the definition of EAQA was defined from EA user viewpoint. The criteria for each quality attribute were developed to compare EAFs using four dimensional concepts. This paper compared several frameworks using the criteria to provide guidance in the selection of an EAF that meets the quality requirement of EA.
Temperature dependence of the thermodynamic functions of strongly interacting liquid alloys (1992)

A model is proposed to obtain expressions for thermodynamic functions like the heat of mixing (HM), excess specific heat ( Delta CP), volume of mixing (VM) and isothermal compressibility ( chi T) of liquid binary alloys with a strong compound-forming tendency in order to cover a large range of temperature. This has been used to analyse the temperature dependence of the free energy of mixing (GM), HM and Delta CP for liquid Li-Sn alloys. The specific heat of the liquid Li7Sn2 alloy has been determined experimentally by drop calorimetric heat content measurements and is found to support the theoretical value, which is based on EMF analysis. The results indicate self-coordination towards the Sn-rich end and strong hetero-coordination in the region 0.33<or=cLi<or=1.0, being maximum around Li3Sn. The influence of the chemical short-range order (CSRO) on the concentration fluctuations, Scc(0), chemical diffusion, D, and order parameter, alpha 1, has been considered. Scc(0) registers a dominant temperature effect in the self-coordinated region, whereas the effect of temperature on the diffusion coefficient is most visible in the hetero-coordinated region.
High-Throughput Measurements of Hydrogel Tissue Construct Mechanics (2009)

Engineered tissues represent a natural environment for studying cell physiology, mechanics, and function. Cellular interactions with the extracellular matrix proteins are important determinants of cell physiology and tissue mechanics. Dysregulation of these parameters can result in diseases such as cardiac fibrosis and atherosclerosis. In this report we present a novel system to produce hydrogel tissue constructs (HTCs) and to characterize their mechanical properties. HTCs are grown in custom chambers and a robotic system is used to indent them and measure the resulting forces. Force measurements are then used to estimate HTC pretension (cellular contractility). Pretension was reduced in a dose-dependent manner by cytochalasin D (CD) treatment; the highest concentration (2microM) resulted in 10-fold decrease. On the other hand, treatment with fetal bovine serum (20%) resulted in approximately threefold increase in pretension. Excellent repeatability and precision were observed in measurements from replicate HTCs. The coefficient of statistical variance of quantified pretension ranged from 7% to 15% (n=4). Due to the small size (4x4x0.8mm) of the HTCs, this system of profiling HTC mechanics can readily be used in high-throughput applications. In particular, it can be used for screening chemical libraries in search of drugs that can alter tissue mechanics.
Cyclodextrin Inclusion Complex Formation and Solid-State Characterization of the Natural Antioxidants α-Tocopherol and Quercetin (2009)

Cyclodextrin (CD) complexation procedures are relatively simple processes, but these techniques often require very specific conditions for each individual guest molecule. Variations of the coprecipitation from aqueous solution technique were optimized for the CD complexation of the natural antioxidants alpha-tocopherol and quercetin. Solid inclusion complex products of alpha-tocopherol/beta-CD and quercetin/gamma-CD had molar ratios of 1.7:1, which were equivalent to 18.1% (w/w) alpha-tocopherol and 13.0% (w/w) quercetin. The molar reactant ratios of CD/antioxidant were optimized at 8:1 to improve the yield of complexation. The product yields of alpha-tocopherol/beta-CD and quercetin/gamma-CD complexes from their individual reactants were calculated as 24 and 21% (w/w), respectively. ATR/FT-IR, 13C CP/MAS NMR, TGA, and DSC provided evidence of antioxidant interaction with CD at the molecular level, which indicated true CD inclusion complexation in the solid state. Natural antioxidant/CD inclusion complexes may serve as novel additives in controlled-release active packaging to extend the oxidative stability of foods.
Einleitung (1996)

Die kommunistischen Staaten Mittel- und Osteuropas waren in den achtziger Jahren neben den repressiven politischen Verhältnissen durch ein zunehmendes Wohlstandsgefälle zu den marktwirtschaftlich orientierten Ländern des Westens gekennzeichnet. Die Systembedingtheit dieser Entwicklung erschien evident, weshalb im Anschluß an die politischen Umwälzungen seit dem Jahre 1989 die Wirtschaftsreformen mit großen Hoffnungen und Ambitionen in Angriff genommen wurden. Wenngleich dabei der Bevölkerung als Korrelat zu politischen auch ökonomische Freiheiten gewährt wurden, blieben die an die Wirtschaftsreformen geknüpften Erwartungen doch (zunächst) unerfüllt. Die Volkswirtschaften Mittel- und Osteuropas erlebten vielmehr eine Wirtschaftskrise, die durch ein fallendes Bruttosozialprodukt, steigende Arbeitslosigkeit und eine andauernde Inflation geprägt war. Die Enttäuschung über die bisherigen Ergebnisse der Systemtransformation wirkte auch auf die politische Landschaft zurück: nationalistische und sozialistische Kräfte — soweit überhaupt je richtig entmachtet — gewinnen an Einfluß. Ein Umschlagen des politischen Gestaltungswillens ist demnach nicht unwahrscheinlich, was ein Scheitern der marktwirtschaftlichen Reformen oder gar eine Restauration ehemaliger Verhältnisse bedeuten würde. Sind also die tiefgreifenden Reformen in den jungen, sich entwickelnden, demokratischen Systemen aufgrund politischer Gegenbewegungen zum Scheitern verurteilt?
Improving Thrust by Suppressing Charge Build-Up in Pulsed DBD Plasma Actuators (2009)

Surface charge buildup and its effect on performance of DBD plasma actuators driven by repetitive voltage pulses added to dc bias were studied. A simplified numerical model showed a good agreement with the experimental data. This confirms that the surface charge is deposited by the ions which drift from the plasma region to the dielectric surface in the applied bias field . Based on the results of surface charge studies, a new configuration of the DBD plasma actuators was proposed – an asymmetric DBD plasma actuator with a slightly conductive dielectric that would deplete the surface charge between the pulses. Two materials that have been considered as slightly conductive dielectrics – zinc oxide and linen based phenolic, showed some promise, but one was too conductive, and the other too resistive. Further search for suitable materials could potentially improve performance of plasma actuators.
Real Time Eye Tracking and Blink Detection with USB Cameras (2005)

A human-computer interface (HCI) system designed for use by people with severe disabilities is presented. People that are severely paralyzed or afflicted with diseases such as ALS (Lou Gehrig's disease) or multiple sclerosis are unable to move or control any parts of their bodies except for their eyes. The system presented here detects the user's eye blinks and analyzes the pattern and duration of the blinks, using them to provide input to the computer in the form of a mouse click. After the automatic initialization of the system occurs from the processing of the user's involuntary eye blinks in the first few seconds of use, the eye is tracked in real time using correlation with an online template. If the user's depth changes significantly or rapid head movement occurs, the system is automatically reinitialized. There are no lighting requirements nor offline templates needed for the proper functioning of the system. The system works with inexpensive USB cameras and runs at a frame rate of 30 frames per second. Extensive experiments were conducted to determine both the system's accuracy in classifying voluntary and involuntary blinks, as well as the system's fitness in varying environment conditions, such as alternative camera placements and different lighting conditions. These experiments on eight test subjects yielded an overall detection accuracy of 95.3%.
Computation of Adalines' Sensitivity to Weight Perturbation (2006)

In this paper, the sensitivity of Adalines to weight perturbation is discussed. According to the discrete feature of Adalines' input and output, the sensitivity is defined as the probability of an Adaline's erroneous outputs due to weight perturbation with respect to all possible inputs. By means of hypercube model and analytical geometry method, a heuristic algorithm is given to accurately compute the sensitivity. The accuracy of the algorithm is verified by computer simulations.
Control strategy for the double-boost converter in continuous conduction mode applied to power factor correction (2002)

This paper deals with the control strategy for the double boost converter in power factor correction (PFC) application, working in continuous conduction mode (CCM). This converter is based on the classic boost converter split into two parallel modules of power processing; each one of these modules process half of the nominal power of the converter. Technical literature concerning this converter does not analyze in detail some very important control aspects and it seems that it is still lacking a general control strategy for such converter. This subject is developed in the present paper. This is done by converter modeling based upon the PWM switch model. The model obtained, verified by simulations, points to a multivariable control system, since the converter output voltages and the control variables (duty cycle of the two active switches) are strongly coupled. The dynamic uncoupling technique is applied in order to overcome this undesirable characteristic and to design the voltage and current compensation loops. Each module of power processing has a current mode controlled PFC circuit with a current-regulation inner loop and a voltage-regulation outer loop and the dynamic uncoupling is performed over the former. Numerical simulation results of the behavior of the double-boost converter for power factor correction application are presented.
Future Fuel Heat Sink Thermal Management System Technologies Tracking Number: 59586 (2006)

A long -range air vehicle was modeled via the integration of various component systems, including an integrated propulsion system comprised of engine, power, thermal management, a nd fuel subsystems, and linked to an airframe model in order to evaluate the vehicle as a system via trade studies. A baseline thermal management system model was constructed using Modelogics’ Inc. Model Engineer, a Visual Basic (VB6) based thermal modelin g toolkit that facilitates the building, running, and analysis of custom component and system models. This baseline thermal management system was defined such that the fuel temperature remained within current JP -8 fuel specification limits, and a resulting air vehicle mission range was determined. The baseline integrated fuel thermal management subsystem model consisted of a fuel tank system and heat exchangers for sinking waste heat loads into the fuel flow stream from the environmental control system, pow er generators, the engine lubrication system, and the fuel pumps. Given increasing future thermal load requirements (i.e., high energy weapon , long duration, high altitude, high Mach flight, etc.), it is critical to develop technologies that will enable hi gher fuel operating temperatures to ensure operational readiness and increased time on station. Trade studies were run with the baseline model in order to compare and determine those technologies that could influence areas of concern relating to high tempe rature fuel stability. Some of these new enabling technologies are examined in this paper, including the use of an in -line fuel deoxygenation unit to allow for higher fuel burn temperatures without increasing engine deposits (coking); new thermally efficie nt fuel pump designs which can reduce waste heat entering the fuel heat sink flow stream while delivering the required flow rates and pressures; and the use of an electric vapor cycle system to allow for a fuel heat sink to cool the environmental control system for the cockpit, avionics and electronics, by eliminating typically used compressor bleed air. These specialized components were modeled and developed into the baseline thermal management system in order to assess the benefits relating to operability and increased vehicle range as determined by this improved system architecture. Those specific temperature and vehicle range deltas from the baseline model were the underlying determination that showed that these new technologies are critical to the succe ss of future, long duration, high speed flight. This paper will further discuss the trade studies and the thought process as to how these enabling technology concepts were developed from the baseline model, as well as some limited details behind each of th e new technologies themselves, including addressing problematic issues specific to increased system component operating temperatures.
NMR Studies Reveal an Unexpected Binding Site for a Redox Inhibitor of AP Endonuclease 1 (2011)

AP endonuclease 1 (APE1) is a multifaceted protein with essential roles in DNA repair and transcriptional regulation. APE1 (ref-1) activates many transcription factors (TF), including AP-1 and NF-κB. While the mechanism of APE1 redox activity remains unknown, it may involve reduction of an oxidized Cys in the TF DNA-binding domain. Several small molecules inhibit APE1-mediated TF activation, including the quinone derivative E3330. It has been proposed some inhibitors bind near C65, a residue suggested to be important for TF activation, but the binding site has not been determined for any inhibitor. Remarkably, NMR and molecular docking studies here reveal E3330 binds in the DNA repair active site of APE1, far removed from C65. Accordingly, AP endonuclease activity is substantially inhibited by E3330 (100 μM), suggesting that E3330 may not selectively inhibit APE1 redox activity in cells, in contrast with previous proposals. A naphthoquinone analogue of E3330, RN7-60, binds a site removed from both C65 and the repair active site. While a detailed understanding of how these inhibitors work requires further studies into the mechanism of redox activity, our results do not support proposals that E3330 binds selectively (and slowly) to locally unfolded APE1 or that E3330 promotes formation of disulfide bonds in APE1. Rather, we suggest E3330 may suppress a conformational change needed for redox activity, disrupt productive APE1-TF binding, or block the proposed redox chaperone activity of APE1. Our results provide the first structural information for any APE1 redox inhibitor and could facilitate development of improved inhibitors for research and perhaps clinical purposes.
The Duke Health Profile (1990)

The Duke Health Profile (DUKE) is a 17-item generic self-report instrument containing six health measures (physical, mental, social, general, perceived health, and self-esteem), and four dysfunction measures (anxiety, depression, pain, and disability). Items were derived from the 63-item Duke-UNC Health Profile, based upon face validity and item-remainder correlations. The study population included 683 primary care adult patients. Reliability was supported by Cronbach's alphas (0.55 to 0.78) and test-retest correlations (0.30 to 0.78). Convergent and discriminant validity were demonstrated by score correlations between the DUKE and the Sickness Impact Profile, the Tennessee Self-Concept Scale, and the Zung Self-Rating Depression Scale. Clinical validity was supported by differences between the health scores of patients with clinically different health problems. Patients with painful physical problems had a DUKE physical health mean score of 58.1, while patients with only health maintenance problems had a mean score of 83.9 (scale: 0.0 = poorest health and 100.0 = best health). Patients with mental health problems had a DUKE mental health mean score of 49.2, in contrast to 75.7 for patients with painful physical problems and 79.2 for those with health maintenance. The DUKE is presented as a brief technique for measuring health as an outcome of medical intervention and health promotion.