gebhart/patrepeval huggingface dataset:
The Pathogenesis of Pneumococcal Otitis Media in Chinchillas and the Efficacy of Vaccination in Prophylaxis (1981)

Acute pneumococcal otitis media was produced experimentally in 100% of chinchillas whose middle ear cavities were inoculated with Streptococcus pneumoniae. Although intranasal inoculation with S. pneumoniae led to otitis media in 21% of animals, 66% of the colonized chinchillas that had negative pressure in the middle ear developed otitis media. Intranasal inoculation with S. pneumoniae followed by intranasal inoculation with influenza A virus led to otitis media in 73% of chinchillas, while inoculation with infleunza A virus alone produced otitis media in 4% of animals. Measurement of middle ear pressure by tympanometry showed the influenza virus-infected chinchillas developed negative middle ear pressure before the appearance of clinical signs of otitis media. While direct inoculation of the middle ear with pneumococci produced a purulent effusion within days after inoculation, intranasal inoculation with both pneumococci and influenza A virus resulted in the rapid appearance of a serous effusion, which became culture-positive for S. pneumoniae during the second week after inoculation. Spontaneous resolution of middle ear infection occurred within three to eight weeks after inoculation and was associated with an increase in the level of type-specific pneumococcal antibody in serum and middle ear effusion. The histopathology of pneumococcal otitis media included purulent middle ear effusion, epithelial metaplasia, and subepithelial edema and hypercellularity. The initial infiltration of the subepithelial space with polymorphonuclear leukocytes was superceded by infiltration with mononuclear leukocytes in animals that were observed for longer than eight weeks. Among untreated animals observed for long periods, production of granulation tissue, development of submucosal fibrosis, and osteoneogenesis were observed. Systemically administered pneumococcal capsular polysaccharide vaccine was effective for prevention of type-specific otitis media in chinchillas when vaccinated animals were challenged by intranasal inoculation with pneumococci followed by deflation of the middle ear. Animals that seroconverted with at least a twofold increase in the level of antibody in serum after vaccination had an 87% lower incidence of otitis media than did unvaccinated animals. Protection was associated with high levels of antibody in serum before intranasal inoculation, and higher antibody levels were found in sterile middle ear effusions than in effusions that contained pneumococci.
Significantly Greater Expression of ER, PR, and ECAD in Advanced-Stage Low-Grade Ovarian Serous Carcinoma as Revealed by Immunohistochemical Analysis (2007)

A 2-tier system that classifies ovarian serous carcinoma (OSC) as low grade or high grade is gaining acceptance. Women with low-grade OSC generally have higher 5-year survival rates than do women with high-grade OSC. We examined the expression of various markers to further understand the molecular differences between low-grade and high-grade OSCs: the potential therapeutic targets or prognostic markers Her-2/neu, estrogen receptor, and progesterone receptor (PR); the metastasis-associated markers cyclin D1 (BCL1), E-cadherin, matrix metalloproteinase (MMP) 2, and MMP-9; and the cell proliferation-associated markers BCL1, Ki-67 antigen (Ki-67), and p53. For this immunohistochemical analysis, we used paraffin-embedded specimens from 47 patients with advanced-stage low-grade OSC and from 49 patients with advanced-stage high-grade OSC. Our results showed that low-grade tumors expressed significantly higher levels of estrogen receptor, PR, and E-cadherin than did high-grade tumors, suggesting the involvement of gonadal steroid hormones, especially in the pathogenesis of low-grade OSC; the PR positivity was also observed in the stromal component of these low-grade tumors. On the other hand, high-grade tumors trended toward increased expression of MMP-9, BCL1, p53, and Ki-67, and robust MMP-9 positivity was observed in the stromal component of these high-grade tumors. These differences may lead to the development of different therapeutic strategies for women with either the low-grade or the high-grade form of OSC.
Iatrogenic Coronary Ostial Stenosis (1969)

In theory, a coronary artery is liable to damage each time a catheter or a cannula is inserted into it. Actually, such complications occur only infrequently. When they do occur, the effects are immediate and easily recognized since the vessel wall is penetrated or perforated.<sup>1</sup>Now, in a recent issue of the<i>Archives of Pathology</i>, Silver et al report on three patients who took months to manifest signs of coronary artery damage induced during cannulation and perfusion.<sup>2</sup>At autopsy the ostia and first 1 to 2 cm of one or both of their coronary arteries were severely stenosed by thickened intima. Wide discontinuities of medial elastic fibers were related to some zones of intimal thickening. The incidence and nature of such late complications is unknown. Silver and associates suggest that a high perfusion pressure caused injury to the vessels of their patients by overdistension; the media was destroyed
The Breadth and Titer of Maternal HIV-1-Specific Heterologous Neutralizing Antibodies Are Not Associated with a Lower Rate of Mother-to-Child Transmission of HIV-1 (2012)

It has been hypothesized that neutralizing antibodies (NAbs) should have broad specificity to be effective in protection against diverse HIV-1 variants. The mother-to-child transmission model of HIV-1 provides the opportunity to examine whether the breadth of maternal NAbs is associated with protection of infants from infection. Samples were obtained at delivery from 57 transmitting mothers (T) matched with 57 nontransmitting mothers (NT) enrolled in the multicenter French perinatal cohort (ANRS EPF CO1) between 1990 and 1996. Sixty-eight (59.6%) and 46 (40.4%) women were infected by B and non-B viruses, respectively. Neutralization assays were carried out with TZM-bl cells, using a panel of 10 primary isolates of 6 clades (A, B, C, F, CRF01_AE, and CRF02_AG), selected for their moderate or low sensitivity to neutralization. Neutralization breadths were not statistically different between T and NT mothers. However, a few statistically significant differences were observed, with higher frequencies or titers of NAbs toward several individual strains for NT mothers when the clade B-infected or non-clade B-infected mothers were analyzed separately. Our study confirms that the breadth of maternal NAbs is not associated with protection of infants from infection.
Identification and Quantification of the Oxidation Products Derived from α-Acids and β-Acids During Storage of Hops (Humulus lupulus L.) (2013)

α-Acids and β-acids, two main components of hop resin, are known to be susceptible to oxygen and degraded during hop storage, although the oxidation products in stored hops have not been fully identified. In this study, we developed a high-performance liquid chromatography (HPLC) analysis method suitable for separation and quantification of the oxidation products. This HPLC analysis clearly proved, for the first time, that humulinones and hulupones are major products in oxidized hops. We are also the first to identify novel 4'-hydroxy-allohumulinones, suggested to be oxidative products of humulinones, by means of NMR spectroscopy and high-resolution mass spectrometry. Using the developed analytical method, changes in α- and β-acids and their oxidation products during hop storage were clearly revealed for the first time.
Validation of the p21-Activated Kinases as Targets for Inhibition in Neurofibromatosis Type 2 (2008)

Neurofibromatosis type 2 (NF2) is a dominantly inherited cancer disorder caused by mutations at the NF2 gene locus. Merlin, the protein product of the NF2 gene, has been shown to negatively regulate Rac1 signaling by inhibiting its downstream effector kinases, the p21-activated kinases (Pak). Given the implication of Paks in tumorigenesis, it is plausible that merlin's tumor suppressive function might be mediated, at least in part, via inhibition of the Paks. We present data indicating this is indeed the case. First, analysis of primary schwannoma samples derived from NF2 patients showed that in a significant fraction of the tumors, the activity of Pak1 was highly elevated. Second, we used shRNAs to knockdown Pak1, 2, and 3 in NIH3T3 cells expressing a dominant-negative form of merlin, NF2(BBA) (NIH3T3/NF2(BBA)), and find that simultaneous knockdown of Pak1-3 in these cells significantly reduced their growth rates in vitro and inhibited their ability to form tumors in vivo. Finally, while attempting to silence Pak1 in rat schwannoma cells, we found that these cells were unable to tolerate long-term Pak1 inhibition and rapidly moved to restore Pak1 levels by shutting down Pak1 shRNA expression through a methylation-dependent mechanism. These data suggest that inhibiting Pak could be a beneficial approach for the development of therapeutics toward NF2. In addition, the finding that the shRNA-mediated Pak1 suppression was silenced rapidly by methylation raises questions about the future application of such technologies for the treatment of diseases such as cancer.
THE USE OF PLATINUM-OXIDE PLATINUM BLACK IN THE CATALYTIC REDUCTION OF AROMATIC HYDROCARBONS. XVII<sup>1</sup> (1928)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE USE OF PLATINUM-OXIDE PLATINUM BLACK IN THE CATALYTIC REDUCTION OF AROMATIC HYDROCARBONS. XVII1Roger Adams and J. R. MarshallCite this: J. Am. Chem. Soc. 1928, 50, 7, 1970–1973Publication Date (Print):July 1, 1928Publication History Published online1 May 2002Published inissue 1 July 1928https://pubs.acs.org/doi/10.1021/ja01394a026https://doi.org/10.1021/ja01394a026research-articleACS PublicationsRequest reuse permissionsArticle Views198Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Multicolor upconverted luminescence-encoded superparticles via controlling self-assembly based on hydrophobic lanthanide-doped NaYF4 nanocrystals (2011)

We demonstrated the conceptual advantage of encoded superparticles (SPs) based on the upconverted luminescence (UCL) spectral signature. In this research, mass fabrication of UCL-encoded SPs has been achieved by employing a facile, robust, and low-cost self-assembly in microemulsion droplets, which offered a convenient, straightforward, and reproducible technique. The barcode information can be predicted and designed by controlling lanthanide (Ln) composition, dopant concentration, and mass content inside SPs. Furthermore, the SPs were encapsulated with an amphiphilic polymer poly(maleic anhydride-alt-1-octadecene) (PMAO), which not only enhances the stability of the UCL-encoded SPs but also provides the biocompatibility for immobilization of biomolecules. In addition, their surface bioactivity was investigated by using protein immunoassay, which indicates the potential application of the UCL-encoded SPs in biodetection and biomedicine.
Wettability Index Determination by Nuclear Magnetic Resonance (2005)

Abstract Knowing the wetting condition of a reservoir at an early stage is crucial for selecting optimum field development options. Paying insufficient attention to the wetting condition e.g. assuming water wet behavior) may result in incorrect STOIIP estimates, and in unexpected dynamic behavior, e.g. under water flooding. A novel method is presented to determine the in-situ wettability of rocks from NMR logs. The method is based on the fact that fluids experience additional relaxation when in direct contact with the rock surface. A reduction of the oil relaxation time away from its bulk value is generally known as a qualitative wettability indicator. Through detailed modeling of the NMR response, this concept has been further developed to provide a quantitative wettability index. It is based on a model for the microscopic distribution of the crude oil and the wetting state of the rock at any given overall saturation. The method requires an NMR measurement on a sample containing two reservoir fluids, i.e. brine and crude oil. Multi-acquisition schemes including diffusion effects make the interpretation more robust, but a normal NMR acquisition suffices and can be made with all available NMR tools (wireline and LWD). The new NMR based method has been extensively verified on core data against standard wettability tests. Application to NMR logs from several fields, and in particular carbonates, show continuous profiles of varying wettability, ranging from fairly oil-wet, through intermediate, to waterwet, all in line with the available core data.
Galectin-3 Is Not an Universal Marker of Malignancy in Thyroid Nodular Disease in Children and Adolescents (2002)

Current methods of research into thyroid nodular disease (TND) based on the polymerase chain reaction (PCR) and reverse-transcription (RT) permit the detection of some markers, even in poorly cellular biological material. The findings from fine-needle aspiration biopsy (FNAB), the most commonly used procedure in TND, do not always correlate with the postoperative histopathological diagnosis, sometimes giving a false negative result. The aim of this present study was to improve the classical cytological evaluation of the material obtained with ultrasound-guided biopsy with a RT-PCR based technique in order to detect carcinoma even in a minimally invasive form. Aspirate from the 30 patients included in the study was smeared for conventional cytology (H+E and MGG staining) and the leftover material in the needle was frozen for subsequent PCR analysis. Fine-needle aspiration specimens were evaluated for the presence of galectin-3 (GAL-3), the most promising molecular marker of malignancy. As a positive control for cells of follicular origin, thyroglobulin (Tg) gene expression was performed. RT-PCR was performed on extracted RNA and with specific primers for the screened genes, based on a one-step reaction with a Biometra PCR machine. Tg expression was observed in 23 aspirates, among which 10 were positive for the expression of GAL-3 [3 cases of PTC, 1 an oxyphilic variant of FTC, 1 an oxyphilic variant of follicular adenoma (FA), 1 a foetal variant of FA, 2 of Hashimoto thyroiditis (HT) and 2 of HT with coexisting FA]. Our results are the first evidence that GAL-3 expression, previously documented in thyroid carcinoma of follicular origin, is also present in Hashimoto thyroiditis. This study reveals some limitations in nodule or multiple nodules of benign character. If the diagnosis of HT is excluded, then the usefulness of the method in the diagnosis of malignancy may still be very high.
Observation of Floquet-Bloch States on the Surface of a Topological Insulator (2013)

The unique electronic properties of the surface electrons in a topological insulator are protected by time-reversal symmetry. Circularly polarized light naturally breaks time-reversal symmetry, which may lead to an exotic surface quantum Hall state. Using time- and angle-resolved photoemission spectroscopy, we show that an intense ultrashort midinfrared pulse with energy below the bulk band gap hybridizes with the surface Dirac fermions of a topological insulator to form Floquet-Bloch bands. These photon-dressed surface bands exhibit polarization-dependent band gaps at avoided crossings. Circularly polarized photons induce an additional gap at the Dirac point, which is a signature of broken time-reversal symmetry on the surface. These observations establish the Floquet-Bloch bands in solids and pave the way for optical manipulation of topological quantum states of matter.
Shared lateral control with on-line adaptation of the automation degree for driver steering assist system: A weighting design approach (2015)

This paper addresses the shared lateral control for both lane-keeping and obstacle avoidance tasks of a driver steering assist system (DSAS). In this work, we propose a novel approach to deal with the interactions between the human (driver) and the machine (DSAS) by introducing into the vehicle system a fictive nonlinear term representing the driver activity. In this way, the actions of the DSAS are computed according to the driver behaviors (actions and intentions). Based on Takagi-Sugeno control technique together with Lyapunov stability tools, the designed controller is able to handle a large range of variation of vehicle longitudinal speed. In particular, this controller can deal with the system state constraints and also the control input saturation. As will be discussed later, the consideration of these constraints into the control design improves significantly the closed-loop performance under various driving situations. The interests of the proposed method are validated by simulations.
<i>Rapid Communication</i> : Somatic Cell Nuclear Transfer in Humans: Pronuclear and Early Embryonic Development (2001)

Human therapeutic cloning requires the reprogramming of a somatic cell by nuclear transfer to generate autologous totipotent stem cells. We have parthenogenetically activated 22 human eggs and also performed nuclear transfer in 17 metaphase II eggs. Cleavage beyond the eight-cell stage was obtained in the parthenogenetic-activated eggs, and blastocoele cavities were observed in six. Three somatic cell-derived embryos developed beyond the pronuclear stage up to the six-cell stage. The ability to create autologous embryos represents the first step towards generating immune-compatible stem cells that could be used to overcome the problem of immune rejection in regenerative medicine.
Analysis of programs with exception-handling constructs (2002)

Analysis techniques, such as control flow, data flow, and control dependence, are used for a variety of maintenance tasks, including regression testing, dynamic execution profiling, and static and dynamic slicing. To be applicable to programs in languages, such as Java and C++ however, these analysis techniques should, to the extent possible, account for the effects of exception occurrences and exception handling constructs. The paper presents techniques to construct intraprocedural and interprocedural representations on which existing techniques can be performed and demonstrates their applicability to several maintenance tasks.
Purification of a Nuclease from <i>Penicillium citrinum</i> (1974)

Abstract A nuclease was purified about 1500-fold with a recovery of 20% from an aqueous extract of culture of a pigmentless mutant VI–10–14 of Penicillium citrinum on wheat bran. The purified preparation was homogeneous on the basis of the criteria of ultracentrifugation and disc gel electrophoresis. The preparation was essentially free of 5′-nucleotidase, non-specific phosphomonoesterase, non-specific phosphodiesterase and 3′-monoester forming nuclease. The preparation hydrolyzed phosphodiester bonds in RNA and DNA to yield 5′-mononucleotides, and also the phosphomonoester bond in 2′- and 3′-AMP to yield nucleoside and inorganic phosphate. The enzyme activities toward these substrates were not separated and relative ratio of their specific activities remained constant throughout the purification, suggesting that a single enzyme was responsible for these activities.
MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation (1996)

A subtractive hybridization strategy was used to isolate putative genes involved in the development of mouse primordial germ cells (PGC). Complimentary DNA was amplified on RNA isolated from the base of the allantois where PGC are located in the 7.5 days post coitum (dpc) mouse embryo. It was then subtracted by hybridization with cDNA amplified on RNA of the anterior region where PGC are absent. A novel gene thus isolated is designated as Mesp1 and encodes a possible transcription factor MesP1 containing a basic helix-loop-helix motif. Its earliest expression was observed at the onset of gastrulation, as early as 6.5 dpc, in the nascent mesodermal cells that first ingressed at the end of the primitive streak. These expressing cells in the lateral and extraembryonic mesoderm showed a wing-shaped distribution. Its initial expression was soon down-regulated at 7.5 dpc before the completion of gastrulation, except at the proximal end of the primitive streak which included the extraembryonic mesoderm and the base of allantois. At 8 dpc, the expression at the base of the allantois moved laterally. This distribution between 7.0 and 8.0 dpc was similar to that of PGC detected by the alkaline phosphatase activity. However, the expression of Mesp1 was down-regulated thereafter, when PGC entered in the migration stage. After birth, Mesp1 expression was detected only in mature testes, but in a different isoform from that expressed in the embryo. Mesp1 was mapped to the mid region of chromosome 7, near the mesodermal deficiency gene (mesd). However, a Southern hybridization study clearly showed that Mesp1 was distinctly different from mesd. The amino acid sequence and its expression pattern suggest that MesP1 plays an important role in the development of the nascent mesoderm including PGC.
PLZF-RAR alpha fusion proteins generated from the variant t(11;17)(q23;q21) translocation in acute promyelocytic leukemia inhibit ligand-dependent transactivation of wild-type retinoic acid receptors. (1994)

Recently, we described a recurrent variant translocation, t(11;17)(q23;q21), in acute promyelocytic leukemia (APL) which juxtaposes PLZF, a gene encoding a zinc finger protein, to RARA, encoding retinoic acid receptor alpha (RAR alpha). We have now cloned cDNAs encoding PLZF-RAR alpha chimeric proteins and studied their transactivating activities. In transient-expression assays, both the PLZF(A)-RAR alpha and PLZF(B)-RAR alpha fusion proteins like the PML-RAR alpha protein resulting from the well-known t(15;17) translocation in APL, antagonized endogenous and transfected wild-type RAR alpha in the presence of retinoic acid. Cotransfection assays showed that a significant repression of RAR alpha transactivation activity was obtained even with a very low PLZF-RAR alpha-expressing plasmid concentration. A "dominant negative" effect was observed when PLZF-RAR alpha fusion proteins were cotransfected with vectors expressing RAR alpha and retinoid X receptor alpha (RXR alpha). These abnormal transactivation properties observed in retinoic acid-sensitive myeloid cells strongly implicate the PLZF-RAR alpha fusion proteins in the molecular pathogenesis of APL.