gebhart/patrepeval huggingface dataset:
Identification, Isolation and Biochemical Characterization of a Phosphopantetheine:Protein Transferase that Activates the Two Type‐I Fatty Acid Synthases of <i>Brevibacterium Ammoniagenes</i> (1997)

Upon heterologous expression of the Brevibacterium ammoniagenes type-I fatty acid synthase FAS-A in Escherichia coli, only the pantetheine-free apoenzyme is synthesized. Activation of FAS-A to its holoform was achieved by transformation with a second B. ammoniagenes gene, PPT1, encoding a type-I FAS-specific phosphopantetheine transferase. PPT1 was identified as a coding sequence located immediately downstream of the second FAS gene present on the B. ammoniagenes genome, fasB. Due to this linkage, PPT1 was part of the cloned fasB DNA region and, consequently, FAS-B but not FAS-A was synthesized as holoFAS in E. coli. PPT1 encodes a protein of 153 amino acids and has a calculated molecular mass of 16,884 Da. The PPT1 gene product contains 25% identical and 42% conserved amino acids compared with the type-II acyl-carrier-protein-activating enzyme of E. coli. Although there is essentially no intergenic region between fasB and PPT1, the PPTase gene is autonomously expressed in E. coli if flanked by 200 bp of its endogenous 5' DNA. The structural independence of Ppt1p was confirmed immunologically, as specific antibodies react with the purified PPTase but not with FAS-B. Overexpression and purification of the His-tagged Ppt1p allowed the in vitro activation of apoFAS-A. This holoenzyme synthesis requires, in addition to Ppt1p, CoA and Mg2+ and leads to a specific FAS activity comparable to that of natural B. ammoniagenes FAS-A. The reactivity of the in vitro-activated FAS-A was verified by the optical FAS assay and by analysis of its in vitro products. In agreement with the known overall colinearity of B. ammoniagenes FAS-B and the Saccharomyces cerevisiae FAS1 and FAS2 gene products, a PPT1-like sequence is also observed at the C terminus of FAS2. However, in contrast to B. ammoniagenes PPT1, this sequence is an integral part of the yeast FAS2 gene. Thus, activation of type-I fatty acid synthases may be accomplished by distinct trans-acting PPTase enzymes and by intrinsic cis-acting PPTase domains.
Right-weight kernels (2006)

DOE has put forth a considerable effort into light-weight kernels for high performance computing, yet there has been a lack of acceptance and use, perhaps due to the limited support for hardware and software. The arguments for light-weight kernels have been based on the problem of interference , i.e. changes in application performance that occur when the operating system pre-empts the application. Nevertheless, using existing, well supported operating systems for HPC systems has been quite successful. The problems with the standard operating systems remain, however, although their impact on applications is still not quantified. At LANL, we have undertaken a research program to determine whether Linux and/or Plan 9 can be used to realize the benefits of light-weight kernels while maintaining the benefits of a full-featured operating system. Specifically, we are evaluating measures that quantify what is "good" in a Light-Weight Kernel. We are using this knowledge to modify Linux and Plan 9 to make them competitive with custom light weight operating systems, in essence, a Right-Weight Kernel. This paper represents both a summary of early results as well as a description of work in progress.
Flux pinning and phase separation in oxygen-rich<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" display="inline"><mml:mrow><mml:msub><mml:mrow><mml:mtext>La</mml:mtext></mml:mrow><mml:mrow><mml:mn>2</mml:mn><mml:mo>−</mml:mo><mml:mi>x</mml:mi></mml:mrow></mml:msub><mml:msub><mml:mrow><mml:mtext>Sr</mml:mtext></mml:mrow><mml:mi>x</mml:mi></mml:msub><mml:msub><mml:mrow><mml:mtext>CuO</mml:mtext></mml:mrow><mml:mrow><mml:mn>4</mml:mn><mml:mo>+</mml:mo><mml:mi>y</mml:mi></mml:mrow></mml:msub></mml:mrow></mml:math> (2008)

We have studied the magnetic characteristics of a series of superoxygenated ${\text{La}}_{2\ensuremath{-}x}{\text{Sr}}_{x}{\text{CuO}}_{4+y}$ samples. As shown in previous work, these samples spontaneously phase separate into an oxygen-rich superconducting phase with a ${T}_{C}$ near 40 K and an oxygen poor magnetic phase that also orders near 40 K. The oxygenated samples studied are highly magnetically reversible even to low temperatures. Although the internal magnetic regions of these samples might be expected to act as pinning sites, our present study shows that they do not favor flux pinning. Flux pinning requires a matching condition between the defect size and the superconducting coherence length. Thus, our results imply that the magnetic regions are too large to act as pinning centers. This also implies that the much greater flux pinning in typical ${\text{La}}_{2\ensuremath{-}x}{\text{Sr}}_{x}{\text{CuO}}_{4}$ materials is the result of nanoscale inhomogeneities that grow to become the large magnetic regions in the superoxygenated materials. The superconducting regions of the phase separated materials are in that sense cleaner and more homogenous than in the typical cuprate superconductor.
Capturing multiview 3D using a lenticular lens (2011)

When creating a multi-view image for multi-view 3D display with one camera, the camera has to be moved from one viewpoint to another viewpoint. However, this method takes long time and can not get images of a moving object. In this paper, we proposed the method for capturing a multi-view image by one step. In order to capture a multi-view image, the lenticular lens is used in our proposed system. The captured multi-view image becomes reverse by using the lenticular lens. Before calculating the best observation distance, a reversal processing is performed. To display a multi-view 3D image by the multi-view image, the same lenticular lens is used. As an experiment result, we got the multi-view image by our proposal system. When we laid the multi-view image under the lenticular lens, a multi-view 3D image with deep feeling was confirmed.
서비스산업의 서비스품질에 관한 연구 (1999)

The aim of this article is to emphasize the importance of quality improvement and suggest the future task of service quality research in our service industry market. To this end, First, literature related to the model about the behavioral consequences of service qualify at the individual customer level and the tool for measuring perceived service quality in the service industry are revisited. Second, the SERVQUAL model is used as a central framework for defining the real meaning and drawing out problems of perceived service quality model in the service industry Third, this article concludes that service quality must be retested in view of developing the theory and practical instruction for our service industry, especially for the new millennium. Finally, this article suggest a research agenda whereby information about individual-level behavioral consequences and business-level service quality can be monitored and linked from customer satisfaction, service quality, and behavioral intention to profit variables.
On the Nonlinear Inexact Uzawa Algorithm for Saddle-Point Problems (2000)

This paper discusses convergence behavior of the nonlinear inexact Uzawa algorithm for solving saddle-point problems presented in a recent paper of Bramble, Pasciak, and Vassilev ( SIAM J. Numer. Anal., 34 (1997), pp. 1072--1092). It is shown that this algorithm converges under a condition weaker than that stated in their paper.
Oriented Molecular Sieve Membranes by Heteroepitaxial Growth (2002)

Heteroepitaxial growth of titanosilicates (ETS-10 and ETS-4) is reported. Using this heteroepitaxial growth, oriented ETS-10/-4 membranes have been fabricated, demonstrating a novel way to achieve preferred orientation of molecular sieve films.
Human HIV Vaccine Trials: Does Antibody-Dependent Enhancement Pose a Genuine Risk? (1992)

Antibody-dependent enhancement is a general in vitro property of enveloped viruses. In certain circumstances, antibody-dependent enhancement is a bona fide pathophysiologic mechanism in vivo. There are several examples of virus disease of humans and animals in which incomplete or partial immunity can lead to enhanced infection and/or disease. In some cases, this appears to be attributable to antibody-dependent enhancement. Conversely, there are several examples of viruses for which in vitro antibody-dependent enhancement has been demonstrated, but for which vaccines have been used safely in millions of persons for decades. Finally, antibody-dependent enhancement of HIV is a genuine concern. However, to date there is no direct clinical, experimental, or epidemiological evidence that HIV enhancement can be operative in vivo. Such evidence should be actively sought.
Contribution of Protease-activated Receptors 1 and 4 and Glycoprotein Ib-IX-V in the Gi-independent Activation of Platelet Rap1B by Thrombin (2004)

Thrombin activates human platelets through three different membrane receptors, the protease-activated receptors PAR-1 and PAR-4 and the glycoprotein Ib (GPIb)-IX-V complex. We investigated the contribution of these three receptors to thrombin-induced activation of the small GTPase Rap1B. We found that, similarly to thrombin, selective stimulation of either PAR-1 or PAR-4 by specific activating peptides caused accumulation of GTP-bound Rap1B in a dose-dependent manner. By contrast, in PAR-1- and PAR-4-desensitized platelets, thrombin failed to activate Rap1B. Thrombin, PAR-1-, or PAR-4-activating peptides also induced the increase of intracellular Ca(2+) concentration and the release of serotonin in a dose-dependent manner. We found that activation of Rap1B by selected doses of agonists able to elicit comparable intracellular Ca(2+) increase and serotonin release was differently dependent on secreted ADP. In the presence of the ADP scavengers apyrase or phosphocreatine-phosphocreatine kinase, activation of Rap1B induced by stimulation of either PAR-1 or PAR-4 was totally inhibited. By contrast, thrombin-induced activation of Rap1B was only minimally affected by neutralization of secreted ADP. Concomitant stimulation of both PAR-1 and PAR-4 in the presence of ADP scavengers still resulted in a strongly reduced activation of Rap1B. A similar effect was also observed upon blockade of the P2Y12 receptor for ADP, as well as in P2Y12 receptor-deficient human platelets, but not after blockade of the P2Y1 receptor. Activation of Rap1B induced by thrombin was not affected by preincubation of platelets with the anti-GPIbalpha monoclonal antibody AK2 in the absence of ADP scavengers or a P2Y12 antagonist but was totally abolished when secreted ADP was neutralized or after blockade of the P2Y12 receptor. Similarly, cleavage of the extracellular portion of GPIbalpha by the cobra venom mocarhagin totally prevented Rap1B activation induced by thrombin in the presence of apyrase and in P2Y12 receptor-deficient platelets. By contrast, inhibition of MAP kinases or p160ROCK, which have been shown to be activated upon thrombin binding to GPIb-IX-V, did not affect agonist-induced activation of Rap1B in the presence of ADP scavengers. These results indicate that although both PAR-1 and PAR-4 signal Rap1B activation, the ability of thrombin to activate this GTPase independently of secreted ADP involves costimulation of both receptors as well as binding to GPIb-IX-V.
Polystyrene and polystyrene‐butadiene latexes stabilized by poly(N‐isopropylacrylamide) (1988)

Abstract Latexes stabilized by poly(N‐isopropylacrylamide) (polyNIPAM) were prepared by polymerizing NIPAM in the presence of polystyrene and polystyrene‐butadiene latex or by styrene emulsion polymerization in the presence of NIPAM. In 0.01 M CaCl 2 polyNIPAM stabilized latexes exhibited critical flocculation temperatures in the range 32–35°C, which is approximately equal to the lower critical solution temperature of polyNIPAM in water. Partial substitution of NIPAM with some acrylamide (AM) gave higher flocculation temperatures. Coagulation studies with cleaned latex indicated that the polyNIPAM or polyNIPAM‐co‐AM polymer chains were anchored to the latex particle surfaces.
There are many good periodically time-varying convolutional codes (1989)

It is shown empirically that the number of good periodically time-varying convolutional codes increases exponentially with the period for any set of parameters. Hence, they can be used to enhance the security of cryptosystems without sacrificing error correction capability. It is shown that some periodically time-varying convolutional codes improve the free distance compared with fixed codes.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Electrooptically active polymers. Non‐linear optical polymers prepared from maleic anhydride copolymers by polymer analogous reactions (1994)

Abstract The synthesis of new high‐molecular‐weight non‐linear optical (NLO) polymers, prepared by polymer analogous reaction of maleic anhydride copolymers with aminoalkyl functionalized azo dyes is described. The thermal behavior of the resulting copolymers is discussed in terms of molar dye concentration, polymer main‐chain flexibility and spacer length on the glass transition temperature.
On the ability of a Darcy-scale model to capture wormhole formation during the dissolution of a porous medium (2002)

Dissolution of a porous medium creates, under certain conditions, some highly conductive channels called wormholes. The mechanism of propagation is an unstable phenomenon depending on the microscopic properties at the pore scale and is controlled by the injection rate. The aim of this work is to test the ability of a Darcy-scale model to describe the different dissolution regimes and to characterize the influence of the flow parameters on the wormhole development. The numerical approach is validated by model experiments reflecting dissolution processes occurring during acid injection in limestone. Flow and transport macroscopic equations are written under the assumption of local mass non-equilibrium. The coupled system of equations is solved numerically in two dimensions using a finite volume method. Results are discussed in terms of wormhole propagation rate and pore volume injected.
Cerebral blood flow changes in benign aging and cerebrovascular disease (1984)

Cross-sectional analysis of CBF values was carried out among 668 volunteers and patients. Subjects were subdivided according to age, gender, and degree of cerebrovascular disease, ranging from healthy volunteers with or without risk factors for stroke to patients with multi-infarct dementia. Four-year longitudinal analysis was also carried out on 230 individuals from the original sample. Decrements in CBF values were evidenced by both cross-sectional and longitudinal analysis in relation to advancing age, progressive cerebrovascular disease, and dementia. Regional, age-related CBF declines in healthy volunteers were heterogeneous, possibly related to changes in levels of functional activity within different brain regions.
Torque Estimation and Misfire Detection using Block Angular Acceleration (2000)

<div class="htmlview paragraph">This work examines the possibility of detecting misfires via measurements of the angular acceleration of the engine block. Measurements were taken on a production 4-cylinder engine which was modeled as a single degree of freedom torsional oscillator. The torque waveform was estimated and compared to the torque calculated via cylinder pressure measurements. Further analysis was conducted in the frequency domain. Results indicate that metrics based on low frequency information were most reliable, but this is impractical for vehicular applications. The accuracy of high frequency metrics was degraded due to the limitations of the model and the non-rigid behavior of the block at high engine speeds.</div>
Miniaturized video-rate epi-third-harmonic-generation fiber-microscope (2010)

With a micro-electro-mechanical system (MEMS) mirror, we successfully developed a miniaturized epi-third-harmonic-generation (epi-THG) fiber-microscope with a video frame rate (31 Hz), which was designed for in vivo optical biopsy of human skin. With a large-mode-area (LMA) photonic crystal fiber (PCF) and a regular microscopic objective, the nonlinear distortion of the ultrafast pulses delivery could be much reduced while still achieving a 0.4 microm lateral resolution for epi-THG signals. In vivo real time virtual biopsy of the Asian skin with a video rate (31 Hz) and a sub-micron resolution was obtained. The result indicates that this miniaturized system was compact enough for the least invasive hand-held clinical use.
Antimicrobial susceptibility of Pseudomonas aeruginosa to 10 antimicrobial agents (2005)

ObjectiveTo investigate the antimicrobial susceptibility of Pseudomonas aeruginosa isolated from hospitalized patients during 2002 to 2004.MethodsDisk diffusion method was used to determine the susceptibility of 368 strains of P.aeruginosa to 10 antibiotics.ResultsOverall,54.3% and 52.2% of P.aeruginosa isolates from ICU patients were resistant to imipemem and meropemem respectively.But the resistance rate of P.aeruginosa isolated from non-ICU inpatients was 25.5% to imipenem and 18.5% to meropenem.The difference was 28.8 and 33.7 percentage points respectively(P0.01).The resistance rate of isolates from ICU patients to cefepime,ceftazidime,aztreonam was also higher than that from non-ICU inpatients.The difference was between 10.2 and 12.6 percentage points(P0.05).The isolates from respiratory tract were more resistant than those from non-respiratory tract to piperacillin,piperacillin-tazobactam,cefepime,imipenem,meropenem,ciprofloxacin,gentamicin and amikacin.The difference of resistance rate ranged from 12.2 to 25.4 percentage points(P0.01).For ceftazidime,the difference of resistance rate was 10.1 percentage points(P0.05).ConclusionsP.aeruginosa isolated from ICU patients and respiratory tract were more resistant to many antibiotics than those isolated from non-ICU inpatients and non-respiratory tract specimens.
Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins (2002)

Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3'-located conserved sequence 1 and an RNA sequence located in the 5' end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
Thermally tunable silicon racetrack resonators with ultralow tuning power (2010)

We present thermally tunable silicon racetrack resonators with an ultralow tuning power of 2.4 mW per free spectral range. The use of free-standing silicon racetrack resonators with undercut structures significantly enhances the tuning efficiency, with one order of magnitude improvement of that for previously demonstrated thermo-optic devices without undercuts. The 10%-90% switching time is demonstrated to be ~170 µs. Such low-power tunable micro-resonators are particularly useful as multiplexing devices and wavelength-tunable silicon microcavity modulators.
Rapeseed Proteins for Paperboard Coating (2014)

Abstract Protein samples of rapeseed were prepared with different compositions as protein isolate, protein concentrate and acetylated protein concentrate. They showed functional properties which are consolidated in the coating. The manufactured protein samples were tested in a pilot‐scale paperboard coating plant. The results indicated that the use of rapeseed proteins in the coating preparation is technically feasible and that the color and roughness of the coated paperboard show no negative effect on the quality. Industry printing experiments with this coated paperboard achieved the standard quality.
Uterine NK Cells Mediate Inflammation-Induced Fetal Demise in IL-10-Null Mice (2005)

Specialized NK cells are recruited in high numbers to the mammalian embryo implantation sites, yet remain pregnancy compatible. It is not well understood whether uterine NK (uNK) cells become adversely activated and mediate fetal demise, a common complication of early pregnancy. In this study we show that mating of IL-10(-/-) mice resulted in fetal resorption or intrauterine growth restriction in response to very low doses of LPS. Pregnancy in congenic wild-type mice was normal even at 10-fold higher LPS doses. Fetal resorption in IL-10(-/-) mice was associated with a significant increase in uNK cell cytotoxic activation and invasion into the placenta. Depletion of uNK cells, TNF-alpha neutralization, or IL-10 administration rescued pregnancy in LPS-treated IL-10(-/-) animals. Our results identify an immune mechanism of fetal demise involving IL-10 deficiency, NK cells, and inflammation. These results may provide insight into adverse pregnancy outcomes in humans.
Second-harmonic generation from individual surface defects under local excitation (2000)

Enhancement of optical second-harmonic generation (SHG) at individual defects on metal surface has been studied. SHG has been excited locally at chosen defects using a near-field optical microscope with an uncoated fiber tip. SH intensity enhancement up to ten times has been observed at the apex of micron size defects on a gold surface while the average enhancement is of about 1.2 times. Observed SHG enhancement has been described by lightning rod effect. Specific features of SHG enhancement due to local excitation are briefly discussed.
A topographically organized gamma‐aminobutyric acid projection from the ventral pallidum to the nucleus accumbens in the rat (1994)

Anatomical and electrophysiological studies have indicated that a reciprocal projection from the ventral pallidum back to the nucleus accumbens exists and has functional relevance. In this study, the topographical projection from the ventral pallidum to the nucleus accumbens was examined by using retrograde tracing with fluoro-gold iontophoresed in subcompartments of the nucleus accumbens in rats combined with either in situ hybridization for glutamic acid decarboxylase and preproenkephalin mRNA or substance P immunoreactivity. Deposits made into the medial nucleus accumbens preferentially labeled neurons in the medial ventral pallidum, while deposits into the dorsolateral nucleus accumbens, at or lateral to the anterior commissure, labeled primarily cells in the dorsal and lateral ventral pallidum. A mediolateral to rostrocaudal topography was also observed, with the medial deposits preferentially labeling cells in rostral ventral pallidum and the lateral deposits resulting in retrogradely labeled cells in the ventral pallidum below the crossing of the posterior anterior commissure (subcommissural) as well as below the globus pallidus (sublenticular). The majority of cells retrogradely labeled with fluoro-gold were double-labeled for glutamic acid decarboxylase mRNA. In contrast, very few retrogradely labeled neurons in the ventral pallidum were double labeled for mRNA for preproenkephalin. These data demonstrate a topographically organized projection from the ventral pallidum to the nucleus accumbens that is primarily gamma-aminobutyric acid (GABA)-ergic and reciprocal to the GABAergic projection from the nucleus accumbens to the ventral pallidum.
Characterization of Mode Coupling in Few-Mode Fibers Using Optical Low-Coherence Reflectometry (2008)

Optical low-coherence reflectometry is used to characterize mode-coupling in few-mode fibers. Besides identification of modes by relative group delays, the technique makes possible the quantification of power coupling between individual fiber modes.
Myocardial Ischemia During Mental Stress: Role of Coronary Artery Disease Burden and Vasomotion (2013)

The degree of digital microvascular constriction, and not the angiographic burden of CAD, is associated with MSIMI. Varying causes of MSIMI compared with PSIMI may require different therapeutic interventions that require further study.