gebhart/patrepeval huggingface dataset:
Organotypic Culture of Human Skin to Study Melanocyte Migration (1994)

An ex vivo model system was developed to investigate melanocyte migration. Within this model system, melanocytes migrate among other epidermal cells in the epibolic outgrowth of skin explants. This process is initiated by loss of contact inhibition of epidermal cells at the rim of the explants and by locally produced chemotactic factors. Punch biopsies provided explants of reproducible diameter. Optimal culture conditions include medium consisting of Dulbecco's Minimal Essential Medium containing 10% inactivated normal human serum and placement of explants epidermal side up at the air-liquid interphase. Within 7 days, epidermal cells completely surround the explant. Approximately 3 days after the onset of keratinocyte migration, melanocytes distribute themselves within the newly formed epidermis. Throughout the 7-day culture period, melanocytes and keratinocytes show maintenance of subcellular morphology, and the dermo-epidermal junction remains intact. Melanocyte migration was quantified using immunoperoxidase staining in combination with light microscopy and computer-aided image analysis. Preliminary results using the model system to compare migration in control and nonlesional vitiligo skin indicate that no inherent migration defect is responsible for impaired repigmentation of vitiligo lesions. The organotypic culture model system allows for investigations on melanocytes within their environment of autologous epidermal and dermal components, closely resembling in vivo circumstances in human skin.
Organometallic nonlinear optical polymers. 3. Copolymerization of bridged bis(ferrocenyl) and bis(cyanoacetate) monomers via the Knoevenagel condensation (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTOrganometallic nonlinear optical polymers. 3. Copolymerization of bridged bis(ferrocenyl) and bis(cyanoacetate) monomers via the Knoevenagel condensationMichael E. Wright and Matthew S. SigmanCite this: Macromolecules 1992, 25, 22, 6055–6058Publication Date (Print):October 1, 1992Publication History Published online1 May 2002Published inissue 1 October 1992https://pubs.acs.org/doi/10.1021/ma00048a031https://doi.org/10.1021/ma00048a031research-articleACS PublicationsRequest reuse permissionsArticle Views256Altmetric-Citations53LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts
Tertiary mammal localities (2010)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Regulation of Channels by the Serum and Glucocorticoid-Inducible Kinase - Implications for Transport, Excitability and Cell Proliferation (2003)

The serum and glucocorticoid-inducible kinase SGK1 stimulates the Na+ channels ENaC and SCN5A, the K+ channels ROMK1, Kv1.3, and KCNE1/KCNQ1, the cation conductance induced by 4F2/LAT1 and the chloride conductance induced by CFTR. The isoforms SGK2 and SGK3 have similarly been shown to regulate ENaC, SCN5A, Kv1.3 and KCNE1/KCNQ1. The kinases regulate channel abundance in the plasma membrane in part by inhibition of the ubiquitin ligase Nedd4-2 and in part by interaction with trafficking molecules such as the Na+/H+ exchanger regulating factor NHERF2. An in vivo role of SGK1 mediated ENaC channel regulation in renal salt excretion and blood pressure control is documented by the impaired ability of SGK1 knockout mice to adequately reduce renal Na+ output and maintain blood pressure during dietary salt restriction and by enhanced blood pressure in individuals carrying certain polymorphisms in the SGK1 gene. The in vivo physiological significance of SGK dependent regulation of the other channels remains to be shown even though circumstantial evidence points to involvement in the regulation of epithelial transport, cell volume, cell proliferation, cardiac action potential and neuroexcitability. There is little doubt that further channels will be identified which are modulated by the SGKs and that further in vivo physiological functions will be defined where channel regulation by the SGKs plays a critical role.
Pedestrian Detection via Classification on Riemannian Manifolds (2008)

We present a new algorithm to detect pedestrian in still images utilizing covariance matrices as object descriptors. Since the descriptors do not form a vector space, well known machine learning techniques are not well suited to learn the classifiers. The space of d-dimensional nonsingular covariance matrices can be represented as a connected Riemannian manifold. The main contribution of the paper is a novel approach for classifying points lying on a connected Riemannian manifold using the geometry of the space. The algorithm is tested on INRIA and DaimlerChrysler pedestrian datasets where superior detection rates are observed over the previous approaches.
Commercial Excimer Lasers (1986)

Excimer lasers form a group of pulsed high-pressure gas lasers which emit at four wave-lengths, namely at 193nm (ArF*), 248nm (KrF*), 308nm (XeCl*) and 351nm (XeF*) in the ultraviolet spectral region. Unlike the infrared radiation from lasers like e.g. Nd:YAG and CO<sub>2</sub>, the high energy photons from excimer lasers typically interact with the electronic excitation levels of an absorber and this can lead to chemical bond breaking, ionization or electronic excitation. The excellent focusability of their short wave-length radiation can further be exploited to generate submicron structures.
Design Study of BOP Shear Rams Based on Validated Simulation Model and Sensitivity Studies (2014)

Blowout Preventers (BOP) are safety devices used to prevent uncontrolled flow of liquids and gases during a blowout. Blind Shear Rams (BSR) is one of the critical components of a BOP responsible for shearing the drill pipe and sealing the wellbore during a blowout scenario. Tests were conducted by shearing a drill pipe under non-flowing conditions to obtain the maximum shearing force, shape of sheared drill pipes, shearing time. A FE methodology has been developed to model the shearing process using Abaqus Explicit finite element solver. Simulations were performed to replicate the shop test and the results are compared with the shop test and found to be in good agreement. The current study uses a validated model for evaluating some of the challenges being faced by the BOP shear ram technology. These include drill pipe centralization in the well bore, shearing of a drill pipe subjected to axial tension, compression and buckling, and shearing in flowing well conditions. All these studies are performed and their effect on shearing process is discussed. The effect of high velocity formation fluid through the drill pipe and annulus in the localized shearing region is also assessed separately by performing Computational Fluid Dynamics (CFD) simulations and it is found that the resistance offered by flowing fluid is not significant compared to the high pressure from accumulators required to shear the pipe. A shear ram design accommodating the results of the study is verified for increased efficiency of the shearing process. The study is conducted as part of a Technology Assessment Programs (TAP) for the Bureau of Safety and Environmental Enforcement (BSEE) in the areas of BOP stack sequencing, monitoring and kick detection.
Über die Beziehungen zwischen der Konstitution und den Eigenschaften von Alkylbenzolsulfonaten mit jeweils einer geraden oder verzweigten Alkylkette bis zu 18 Kohlenstoff‐Atomen I (1955)

Abstract Technisch wichtige Eigenschaften definierter, reiner Alkylsulfonate und einiger binärer Gemische wurden eingehend untersucht: Kritische Micellbildungskonzentration, Erniedrigung der Oberflächenspannung, Benetzungs‐, Schaum‐ und Waschvermögen. Die erhaltenen Ergebnisse werden ausführlich diskutiert.
Whole-Surface Analysis of Semiconductor Wafers by Accumulating Short-Time Mapping Data of Total-Reflection X-ray Fluorescence Spectrometry (2002)

Total-reflection X-ray fluorescence (TXRF) spectrometry with no chemical preconcentration, often called "straight-TXRF", is now widely used in the semiconductor industry. The small detection area of TXRF enablesmapping measurement of contamination of the semiconductor surface, which is very useful in process characterization. However, the small detection area had been believed to limit rapid whole-surface analysis. Contrary to this general understanding, in this study we demonstrated that a new method, called "sweeping-TXRF", which is essentially short-time multipoint mapping by straight-TXRF, can rapidly provide an average concentration. A considerable problem of this method is the contribution of errors in glancing angle and areal element distribution to the fluorescence. Using statistics, we examined the errors and demonstrated that most of them are canceled and are not significant in actual semiconductor applications. The results of an experiment that measured localized 6 x 10(10) atoms cm(-2) nickel contamination supported the above conclusion. Applying sweeping-TXRF to existing TXRF instruments is easy-the only requirement is a small software modification. We believe that sweeping-TXRF will be utilized for rapid whole-surface analysis in many fields, especially in the semiconductor industry.
Nefopam and morphine in man (1975)

A new analgesic, nefopam, is chemically distinct and pharmacologically unrelated to any presently known analgesic. A comparison was made of morphine and nefopam in 74 patients who required parenteral analgesia for moderate to severe postoperative and somatic pain, using a single administration, 2-dose level, double-blind design. A significant dose-response curve was obtained with nefopam and with morphine, and there was no significant deviation from parallelism. The time-effect curves for the 2 drugs were similar. The estimated relative potency of nefopam to morphine indicates that 20 mg of nefopam HCl is the approximate analgesic equal of 12 mg of morphine SO4. There were no adverse effects with nefopam and one adverse reaction to morphine.
<title>Acquisition time calculation and influence of vibrations for microsatellite laser communication in space</title> (2001)

The first step in creating an optical link between two LEO satellites is acquisition. In this process one of the satellites finds the maximal power of a received beam and locks on to it. This starts the tracking. In this paper we examine the time needed to finish the acquisition process and start tracking. The parameters included are the distribution function of satellite position, the size of the uncertainty area, the number of possible satellite positions, and the detection ability of a CCD. A model for the distribution function of position is given for two types of distribution: Gaussian and Uniform. Also considered the vibrations that come from internal systems of satellite, and from external sources. The characteristics of vibrations are considered and their influence on the scanning pattern that can deviate from the original path. A method of filtering the vibrations and compensating for them is suggested. The pointing system must be updated continuously from the star tracker with internal calculations of position, speed, velocity and vibrations characteristics. Examined also are several scanning methods: raster, spiral, Lissajo, Rose. Each method has its own possibilities and advantages, which are compared.
Nicotine Induces Negative Energy Balance Through Hypothalamic AMP-Activated Protein Kinase (2012)

Smokers around the world commonly report increased body weight after smoking cessation as a major factor that interferes with their attempts to quit. Numerous controlled studies in both humans and rodents have reported that nicotine exerts a marked anorectic action. The effects of nicotine on energy homeostasis have been mostly pinpointed in the central nervous system, but the molecular mechanisms controlling its action are still not fully understood. The aim of this study was to investigate the effect of nicotine on hypothalamic AMP-activated protein kinase (AMPK) and its effect on energy balance. Here we demonstrate that nicotine-induced weight loss is associated with inactivation of hypothalamic AMPK, decreased orexigenic signaling in the hypothalamus, increased energy expenditure as a result of increased locomotor activity, increased thermogenesis in brown adipose tissue (BAT), and alterations in fuel substrate utilization. Conversely, nicotine withdrawal or genetic activation of hypothalamic AMPK in the ventromedial nucleus of the hypothalamus reversed nicotine-induced negative energy balance. Overall these data demonstrate that the effects of nicotine on energy balance involve specific modulation of the hypothalamic AMPK-BAT axis. These targets may be relevant for the development of new therapies for human obesity.
Donor activating KIR3DS1 is associated with decreased acute GVHD in unrelated allogeneic hematopoietic stem cell transplantation (2010)

The natural killer cell receptor KIR3DS1 is associated with improved outcome in malignancies, infections, and autoimmune diseases, but data for the impact of KIR3DS1 in HSCT are inconsistent. Using genomic DNA from the National Marrow Donor Program, we performed donor KIR genotyping for 1087 patients who received an unrelated hematopoietic stem cell transplantation. A total of 33% of donors were KIR3DS1(+). Compared with KIR3DS1(-) donors, donor KIR3DS1 was associated with lower-grade II-IV acute graft-versus-host disease (GVHD; odds ratio = 0.71; 95% confidence interval, 0.55-0.92; P = .009), but not with relapse (hazard ratio = 0.97; 95% confidence interval, 0.73-1.29; P = .82). Furthermore, grade II-IV acute GVHD, overall mortality, and transplantation-related mortality all decreased as the number of copies of donor KIR3DS1 increased (P = .007, P = .03, and P = .02, respectively), with the lowest failure rate occurring among patients homozygous for donor KIR3DS1. Selection of donors with KIR3DS1 may decrease acute GVHD without compromising relapse-free survival, separating the graft-versus-tumor effect from unwanted GVHD.
Hard Disk Drive Spindle Motor System Design For Data Recording With Ultrahigh TPI (2009)

The performance of spindle motor system is crucial to that of the hard disk drive (HDD). The spindle motor system faces tremendous challenges as the HDD industry keeps pushing up magnetic recording areal density. This paper analyzes several attributes of the spindle motor system which affect the recording density of the hard disk drive. Some of the attributes have not been highlighted and studied before. The present analysis shows that the performance of spindle motor system can be improved through the integration of optimal electromagnetic design, mechanical system enhancement and advanced power drive to meet the requirements of the next-generation hard disk drive with ultrahigh areal density.
RNA and DNA Hydrolysis Are Catalyzed by the Influenza Virus Endonuclease (2000)

The influenza virus polymerase complex contains a metal ion-dependent endonuclease activity, which generates short capped RNA primer molecules from capped RNA precursors. Previous studies have provided evidence for a two-metal ion mechanism of RNA cleavage, and the data are consistent with a direct interaction of a divalent metal ion with the catalytic water molecule. To refine the model of this active site, we have generated a series of DNA, RNA, and DNA-RNA chimeric molecules to study the role of the 2'-hydroxy groups on nucleic acid substrates of the endonuclease. We could observe specific cleavage of nucleic acid substrates devoid of any 2'-hydroxy groups if they contained a cap structure (m7GpppG) at the 5'-end. The capped DNA endonuclease products were functional as primers for transcription initiation by the influenza virus polymerase. The apparent cleavage rates were about 5 times lower with capped DNA substrates as compared with capped RNA substrates. Cleavage rates with DNA substrates could be increased to RNA levels by substituting the deoxyribosyl moieties immediately 5' and 3' of the cleavage site with ribosyl moieties. Similarly, cleavage rates of RNA substrates could be lowered to DNA levels by exchanging the same two ribosyl groups with deoxyribosyl groups at the cleavage site. These results demonstrate that the 2'-hydroxy groups are not essential for binding and cleavage of nucleic acids by the influenza virus endonuclease, but small differences of the nucleic acid conformation in the endonuclease active site can influence the overall rate of hydrolysis. The observed relative cleavage rates with DNA and RNA substrates argue against a direct interaction of a catalytic metal ion with a 2'-hydroxy group in the endonuclease active site.
Mean Platelet Volume. The Inverse Relation of Platelet Size and Count in Normal Subjects, and an Artifact of Other Particles (1981)

We measured whole-blood mean platelet volume (MPV) and platelet count, determined by the Coulter Counter model S-Plus, in 683 normal subjects. There was nonlinear, inverse relation between MPV and platelet count throughout the normal range of platelet count: the change in MPV was most pronounced at the lower platelet counts. Because of this non-linear relation, "platelet-crit," the product of platelet count and MPV, was not constant, but varied directly with platelet count. Thus, the definition of "normal" values for MPV requires simultaneous reference to platelet count. In the whole blood of 13 patients, non-platelet cell fragments spuriously increased the automated platelet count. Seven patients with erythrocyte fragmentation had spuriously high MPV also: correct MPV and platelet count could be obtained from platelet rich plasma (PRP). In five patients with leukemic blast fragmentation, MPV remained spuriously low and platelet count spuriously high in PRP: only phase count allowed correct count. A patient with megakaryoblastic leukemia had megakaryoblast fragments confused for platelets.
P(PhCH<sub>2</sub>NCH<sub>2</sub>CH<sub>2</sub>)<sub>3</sub>N Catalysis of Mukaiyama Aldol Reactions of Aliphatic, Aromatic, and Heterocyclic Aldehydes and Trifluoromethyl Phenyl Ketone (2009)

Herein we find that proazaphosphatrane 1c is a very efficient catalyst for Mukaiyama aldol reactions of aldehydes with trimethylsilyl enolates in THF solvent. Only the activated ketone 2,2,2-trifluoroacetophenone underwent clean aldol product formation with a variety of trimethylsilyl enolates under similar conditions as the aldehydes. The reactions were carried out at room temperature using (1-methoxy-2-methyl-1-propenyloxy)trimethylsilane, whereas the temperature was -15 degrees C in the case of 1-phenyl-1-(trimethylsilyloxy)ethylene. The reaction conditions are mild and operationally simple, and a variety of aryl functional groups, such as nitro, amino, ester, chloro, trifluoromethyl, bromo, iodo, cyano, and fluoro groups, are tolerated. Product yields are generally better than or comparable to those in the literature. 1-Phenyl-1-(trimethylsilyloxy)ethylene, 1-(trimethylsilyloxy)cyclohexene, and 2-(trimethylsilyloxy)furan underwent clean conversion to beta-hydroxy carbonyl compounds under our reaction conditions. In the case of bulky (2,2-dimethyl-1-methylenepropoxy)trimethylsilane, only alpha,beta-unsaturated esters were isolated. Heterocyclic aldehydes, such as pyridine-2-carboxaldehyde, benzofuran-2-carboxaldehyde, benzothiophene-2-carboxaldehyde, and 1-methyl-2-imidazolecarboxaldehyde, gave good yields of Mukaiyama products. An optimized synthesis for the catalyst 1c is also reported herein.
A Loss of Function Mutant of the Presenilin Homologue SEL-12 Undergoes Aberrant Endoproteolysis in Caenorhabditis elegans and Increases Aβ42 Generation in Human Cells (2000)

The familial Alzheimer's disease-associated presenilins (PSs) occur as a dimeric complex of proteolytically generated fragments, which functionally supports endoproteolysis of Notch and the beta-amyloid precursor protein (betaAPP). A homologous gene, sel-12, has been identified in Caenorhabditis elegans. We now demonstrate that wild-type (wt) SEL-12 undergoes endoproteolytic cleavage in C. elegans similar to the PSs in human tissue. In contrast, SEL-12 C60S protein expressed from the sel-12(ar131) allele is miscleaved in C. elegans, resulting in a larger mutant N-terminal fragment. Neither SEL-12 wt nor C60S undergo endoproteolytic processing upon expression in human cells, suggesting that SEL-12 is cleaved by a C. elegans-specific endoproteolytic activity. The loss of function of sel-12 in C. elegans is not associated with a dominant negative activity in human cells, because SEL-12 C60S and the corresponding PS1 C92S mutation do not interfere with Notch1 cleavage. Moreover, both mutant variants increase the aberrant production of the highly amyloidogenic 42-amino acid version of amyloid beta-peptide similar to familial Alzheimer's disease-associated human PS mutants. Our data therefore demonstrate that the C60S mutation in SEL-12 is associated with aberrant endoproteolysis and a loss of function in C. elegans, whereas a gain of misfunction is observed upon expression in human cells.
The perceived impact of Australian community banks on quality of life (2008)

The article examines the perceived impact of the community banks on the well-being and quality of life of individuals, and whether the perceived impacts differ in any way from those of more conventional banks. It notes that community banks represent a unique partnership between commercial interests and the members of communities in which they are established. It contends that the socio-economic impact of community banks has been found to be very positive in terms of retained capital, spending and employment.
First Trimester Biochemical Screening for Trisomy 21: The Role of Free Beta hCG, Alpha Fetoprotein and Pregnancy Associated Plasma Protein A (1994)

The potential efficacy of screening for trisomy 21 in the first trimester, using maternal serum markers alpha fetoprotein, free beta human chorionic gonadotropin, unconjugated oestriol and pregnancy associated plasma protein A, was studied in an unselected population of women between the seventh and fourteenth week of gestation. Using a combination of alpha fetoprotein and free beta human chorionic gonadotropin, 53% of affected pregnancies could be identified at a false positive rate of 5%. Unconjugated oestriol and pregnancy associated plasma protein A levels were lower in cases of trisomy 21, but their inclusion with other markers did not significantly improve detection rate. Monitoring the same pregnancies also in the second trimester showed that screening in the first trimester identified the same cases as in the second. We conclude that first trimester screening using free beta human chorionic gonadotropin and alpha fetoprotein, is a viable possibility and will lead to detection rates in excess of 50%. Prospective studies are needed to confirm these observations.