gebhart/patrepeval huggingface dataset:
Photocrosslinking analysis of protein-RNA interactions in E. coli transcription complexes. (1993)

Regulation of transcription involves numerous specific protein-nucleic acid interactions. We have utilized photochemical crosslinking to identify interactions between Escherichia coli transcription proteins and the nascent RNA in several transcription complexes, including initiation, elongation, and antitermination complexes. We have developed new nucleotide analogs, 5-APAS-UTP and 5-APAS-CTP, which are tagged with photocrosslinking groups on base positions that do not interfere with normal Watson-Crick base-pairing. These analogs are incorporated at internal positions in RNA by E. coli RNA polymerase without disrupting RNA secondary structures. We have also used 8-azido-ATP, which can be incorporated uniquely into the 3' end of the RNA, to analyze interactions at the enzyme active site. Interactions between the RNA and the polymerase subunits, and the effect of various transcription factors, including NusA, NusB, NusE, and NusG, have been examined in complexes containing RNAs from 4 to approximately 80 nucleotides. At almost every RNA position examined, both the beta and beta' subunits are contacted, but never the alpha subunit or NusA. An effect of NusA on the core labeling has been observed in some complexes, however. Sigma is contacted by nucleotides within three nucleotides of the +1 position on the DNA.
A Novel Mobile Phone Application for Pulse Pressure Variation Monitoring Based on Feature Extraction Technology: A Method Comparison Study in a Simulated Environment (2016)

Our findings show that the Capstesia PPV calculation is a dependable substitute for standard manual PPV determination in a highly controlled environment (simulator study). Further studies are warranted to validate this mobile feature extraction technology to predict fluid responsiveness in real conditions.
One-Step Synthesis of Oxodimethylenemethane−Transition Metal Complexes and Palladium-Catalyzed Cycloaddition Reaction (1996)

Some alkyl allyl carbonates and an allylammonium chloride bearing (1-(butyloxy)ethyl)oxy group at the 2-position of the allyl group were synthesized and successfully transformed to oxodimethylenemethane−palladium and −platinum complexes in one step by mixing with a transition metal(0) and triphenylphosphine. On the basis of the confirmation of vinyl ether formation by 1H NMR, the generation of oxodimethylenemethane complexes was rationalized to occur through abstraction of the β-hydrogen on the acetal carbon by an alkoxide ion which was generated from the allyl carbonate upon oxidative addition of the transition metal. The palladium-catalyzed cycloaddition of the acetonylidene group to strained olefins also proceeded successfully by using these alkyl allyl carbonates.
Geo Viser. Geographic visualization of search engine results (1999)

Successful navigation of the web for relevant information is facilitated by keyword and subject based search tools. To the best of our knowledge, most tools which achieve this goal are not designed to find the geographic location of information sources. In many instances such information could prove to be extremely useful to answer queries with certain geo-spatial elements. The main contribution of this paper is the idea of discovering the geographic location of webpages obtained via a search engine query and visualizing the results by placing them on a map in an attempt to convey spatial attributes such as location and distance. Current search engines ignore the existence of such geographic meta-data and therefore are unable to process queries which are inherently geo-spatial in nature. Our system (Geo Viser) integrates this functionality to supplement the current capabilities of a search engine.
Ambulatory Blood Pressure Monitoring In A Nonacademic Setting: Effects of Age and Sex (1992)

Twenty-four-hour ambulatory blood pressure measurements (ABPM) are likely to eliminate the stress of visits and observer bias in office blood pressure (BP) recordings, allow consideration of the circadian variability in BP, and correlate well with target organ damage. To define the prevalence of "white coat" hypertension in a rural community to a nonacademic setting, and to assess age and sex related differences, we studied 131 patients who had more than two prior office diastolic BP measurements greater than 90 mm Hg and less than 115 mm Hg. Blood pressure was measured every 10 to 60 min for 24 h using the SpaceLabs 90207 device. Office BP readings were higher than ABPM in the group as a whole, in individual age groups, and in both sexes. The differences were more pronounced at night. Average differences between office and ambulatory BP ranged between 14.4 +/- 1.7/2.9 +/- 2.0 (ABPM at 10:00), and 33.8 +/- 2.3/22.8 +/- 1.5 mm Hg (systolic/diastolic +/- SE) (ABPM at 01:00). The nighttime drop in systolic BP was not apparent in subjects more than 65 years old. Women had a proportionately higher mean office BP than men (115.0 +/- 0.9 office v 110.2 +/- 1.3 mm Hg ABPM in women and 112.3 +/- 0.9 v 104.3 +/- 1.1 mm Hg in men) (P = .013), and the elderly did not display the relationship between ambulatory and office mean BP seen in younger subjects (r = 0.15, P = .30 v r = 0.36, P = .0004, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
Elevated Fibrin <i>D</i>-Dimer Fragment in Sickle Cell Anemia: Evidence for Activation of Coagulation during the Steady State as well as in Painful Crisis (1989)

The fibrin D-dimer degradation fragment is produced by plasmin degradation of cross-linked fibrin. Elevated plasma D-dimer levels indicate increased plasmin degradation of cross-linked fibrin, and are therefore an indirect indication of increased thrombin activity and fibrin formation. With an ELISA assay sensitive to plasma D-dimer levels of less than 50 ng/ml, elevated levels of D-dimer were found in 23 of 25 subjects with sickle cell disease tested during the steady state, and in all 21 subjects tested serially while hospitalized for painful crisis (total of 40 samples). Mean D-dimer was 79 +/- (SD) 25 ng/ml in 35 normal subjects, 566 +/- 739 ng/ml in sickle cell disease subjects in the steady state, and 1,038 +/- 1,010 ng/ml in sickle cell subjects during painful crisis; these differences were highly significant (p less than 0.001). No correlation was observed between levels of D-dimer in the steady state and the time since the last painful crisis; D-dimer was elevated in all 7 patients tested more than 6 months after their last painful crisis. Most steady-state patients did not have other microvascular occlusive manifestations, such as active leg ulcers or aseptic necrosis of bone, which could explain the increased D-dimer levels observed. It is concluded that increased thrombin activity and fibrin formation are features of steady-state sickle cell disease, and that they further increase during painful crisis. Possible causes and implications of increased thrombin activity and fibrin formation in subjects with sickle cell disease are discussed.
The Chromosomal Arsenic Resistance Genes of <i>Thiobacillus ferrooxidans</i> Have an Unusual Arrangement and Confer Increased Arsenic and Antimony Resistance to <i>Escherichia coli</i> (2000)

The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, and a putative arsR gene, were identified. The T. ferrooxidans arsB (arsenite export) and arsC (arsenate reductase) gene products were functional when they were cloned in an Escherichia coli ars deletion mutant and conferred increased resistance to arsenite, arsenate, and antimony. Therefore, despite the fact that the ars genes originated from an obligately acidophilic bacterium, they were functional in E. coli. Although T. ferrooxidans is gram negative, its ArsC was more closely related to the ArsC molecules of gram-positive bacteria. Furthermore, a functional trxA (thioredoxin) gene was required for ArsC-mediated arsenate resistance in E. coli; this finding confirmed the gram-positive ArsC-like status of this resistance and indicated that the division of ArsC molecules based on Gram staining results is artificial. Although arsH was expressed in an E. coli-derived in vitro transcription-translation system, ArsH was not required for and did not enhance arsenic resistance in E. coli. The T. ferrooxidans ars genes were arranged in an unusual manner, and the putative arsR and arsC genes and the arsBH genes were translated in opposite directions. This divergent orientation was conserved in the four T. ferrooxidans strains investigated.
Reinforcement Learning for Mixing Loop Control with Flow Variable Eligibility Trace (2019)

Mixing Loops are often used for proper pressurization and temperature control in building thermal systems. Optimal control of the mixing loop maximizes comfort while minimizing cost. To ensure optimal control for mixing loops in a wide range of different buildings with different load conditions, a self learning controller is here proposed. The controller uses Reinforcement Learning with flow variable eligibility trace. The controller is shown to improve performance of the mixing loop control compared to state of the art reinforcement learning and industrial grade controllers. The controller is tested on a hardware in the loop setup for rapid testing of mixing loop control used in building heating.
Secure and privacy-preserving information aggregation for smart grids (2011)

In this paper, we present a distributed incremental data aggregation approach, in which data aggregation is performed at all smart meters involved in routing the data from the source meter to the collector unit. With a carefully constructed aggregation tree, the aggregation route covers the entire local neighbourhood or any arbitrary set of designated nodes with minimum overhead. To protect user privacy, homomorphic encryption is used to secure the data enroute. Therefore, all the meters participate in the aggregation, without seeing any intermediate or final result. In this way, our approach supports efficient data aggregation in smart grids, while fully protecting user privacy. This approach is especially suitable for smart grids with repetitive routine data aggregation tasks.
Carbon Dioxide Based Metal Working Fluids. (2008)

Metalworking fluids (MWFs) are necessary for most machining operations to ensure proper cooling and lubrication. Conventional mixtures of water, emulsified oils and additives can lead to significant environmental impacts and worker health risks. Delivery of MWFs in minimum quantities using compressed air reduces many of these impacts but the cooling capacity of these sprays is lower than aqueous MWFs. In this research, a novel class of MWFs delivered in carbon dioxide was developed that is environmentally preferable and capable of providing cooling on par with conventional fluids. Above its critical point, CO2 dissolves many common MWF lubricants and can produce a spray of frozen lubricant and dry ice when delivered through a nozzle. The goal of this research was to evaluate the feasibility of supercritical CO2 (scCO2)-based MWFs for manufacturing applications. A prototype for delivery of scCO2-based MWF was developed and proved capable of reducing friction significantly relative to conventional MWFs. Research on the delivery of scCO2–based MWFs indicated that the lubricating potential is easily “tunable” to meet the needs of different machining operations. To test the cooling capacity of scCO2-based MWFs, heat-induced diffusive tool wear was evaluated in high-speed cutting of hard metals. The results yielded compelling reductions in tool flank wear provided by scCO2-based sprays when delivered directly to the flank surface. The results also illustrated the importance of nozzle geometry, proximity of tool and workpiece, and gas pressure for effective heat dissipation. To evaluate the environmental impacts associated with switching to scCO2-based MWFs, a life cycle analysis (LCA) was performed to compare them with water- and air-based systems. It was found that dramatic reductions are possible in aquatic toxicity, water use, and solid waste when switching from water to scCO2-based MWFs. The switch results in increased greenhouse gas emissions but the magnitude of the increase is small compared to other factory operations and can vary depending on allocation strategies. Taken together, the research reveals that scCO2-based MWFs have great potential to improve manufacturing process performance, while reducing tooling costs and the most prominent worker health risks and environmental impacts associated with MWFs today.
Warped discrete-Fourier transform: Theory and applications (2001)

In this paper, we advance the concept of warped discrete-Fourier transform (WDFT), which is the evaluation of frequency samples of the z-transform of a finite-length sequence at nonuniformly spaced points on the unit circle obtained by a frequency transformation using an allpass warping function. By factorizing the WDFT matrix, we propose an exact computation scheme for finite sequences using less number of operations than a direct computation. We discuss various properties of WDFT and the structure of the factoring matrices. Examples of WDFT for first- and second-order allpass functions is also presented. Applications of WDFT included are spectral analysis, design of tunable FIR filters, and design of perfect reconstruction filterbanks with nonuniformly spaced passbands of filters in the bank. WDFT is efficient to resolve closely spaced sinusoids. Tunable FIR filters may be designed from FIR prototypes using WDFT. In yet another application, warped PR filterbanks are designed using WDFT and are applied for signal compression.
Development of Silica Based Microgels and Evaluation of Their Performance in Microparticle Retention System (2000)

The effectiveness of silica-based microgels prepared through the reaction of sulfuric acid and sodium silicate as a component of Compozil system has been evaluated . Silica based microgels with better performance in retention and drainage than a commercial colloidal silica sol have been successfully prepared. Silica gels with the highest charge density were obtained when product pH was controlled to 9. And highly charged silica-based microgels showed greater retention and freeness performance than a commerical product. In particular the difference in retention, turbidity , and freeness between these microgels and a commercial product was eminent at low addition rate. The effects of reaction conditions including reaction temperature, process water quality and feeing rate on product efficiency in improving retention and drainage were also investigated and discussed.
Silk fibroin linked Zn/Cd-doped SnO<sub>2</sub>nanoparticles to purify the organically polluted water (2018)

Photocatalytic degradation of toxic organic pollutant is a challenging tasks for environmental protection. Recently most of the researchers are focusing on metal oxide semiconductors, such as TiO2, ZnO, and SnO2 as efficient photocatalysts for photocatalytic degradation of organic pollutant. In this regard, we are eager to increase the photocatalytic activity with the help of organic molecule (Silk Fibroin (SF)). Metal oxides are attached on the surface of SF with the help of polyvinyl alcohol (PVA). In this present work, pure SnO2, (Cd/Zn) doped SnO2 and SF linked nanoparticles have been successfully synthesized by microwave technique. The synthesized samples were investigated by XRD, FTIR and UV-DRS techniques. The surface morphology of all the samples were investigated by FESEM analysis. Photocatalytic activities of Cd doped SnO2 nanoparticles and SF attached Cd/Zn doped SnO2 nanoparticles were studied on methylene blue (MB) dye under UV–vis light illumination.
Role of Methanol Sacrificing Reagent in the Photocatalytic Evolution of Hydrogen (2012)

The contribution of methanol (CH3OH) sacrificing reagent to the photocatalytic evolution of H2 from aqueous solutions has been studied by tracing the reaction of D2O over a Cu/S-TiO2 catalyst under UV illumination. Use of D2O/CH3OH produced higher formation rates of HD and D2 than that of H2. The low H2 formation rates indicate that the direct reaction of CH3OH with photogenerated holes does not proceed to an appreciable extent in the presence of high concentrations of D2O. The role of CH3OH in accelerating hydrogen formation can be attributed to its ability to produce an electron donor, injecting its electrons to the conduction band.
Designing an audio channel for low-bandwidth mobile optical networks (2009)

Free-space optical communication links are well established as high bandwidth data transfer facilities. They also provide secure communication in hostile environments where radio frequency-based communication can be easily tapped into or jammed by a hostile agent. Mobile optical communication will thus provide a highly desirable system in such an environment, e.g., a battlefield. This paper presents the design and performance of a system for providing mobile voice communication that is based on optical links. The voice channel is created over two field programmable gate array (FPGA) development boards which allow parameters to be changed as needed. These parameters are chosen so that the voice channel emulates the G.711 ITU-T standard. Several configurations with different data rates can be obtained by varying the sampling rate and the number of bits representing each sample so as to optimize the transmission parameters from both fidelity and dynamic range perspectives. The Quality of the audio channel for each configuration is determined by measuring the Mean Opinion Score (MOS) using an Agilent Voice Quality Tester (VQT). Other parameters such as echo and delay are also measured.
Endoscopic ultrasound‐guided ethanol injection in the pancreas in a porcine model: A preliminary study (2007)

EUS-guided ethanol injection in the pancreas seems to be technically simple. More detailed assessments of the safety and dose-effect relationship issues associated with this procedure are required.
Selection and Characterization of Hepatitis C Virus Replicons Dually Resistant to the Polymerase and Protease Inhibitors HCV-796 and Boceprevir (SCH 503034) (2008)

HCV-796 is a nonnucleoside inhibitor of the hepatitis C virus (HCV) nonstructural protein 5B (NS5B) polymerase, and boceprevir is an inhibitor of the NS3 serine protease. The emergence of replicon variants resistant to the combination of HCV-796 and boceprevir was evaluated. Combining the inhibitors greatly reduced the frequency with which resistant colonies arose; however, some resistant replicon cells could be isolated by the use of low inhibitor concentrations. These replicons were approximately 1,000-fold less susceptible to HCV-796 and 9-fold less susceptible to boceprevir. They also exhibited resistance to anthranilate nonnucleoside inhibitors of NS5B but were fully sensitive to inhibitors of different mechanisms: a pyranoindole, Hsp90 inhibitors, an NS5B nucleoside inhibitor, and pegylated interferon (Peg-IFN). The replicon was cleared from the combination-resistant cells by extended treatment with Peg-IFN. Mutations known to confer resistance to HCV-796 (NS5B C316Y) and boceprevir (NS3 V170A) were present in the combination-resistant replicons. These changes could be selected together and coexist in the same genome. The replicon bearing both changes exhibited reduced sensitivity to inhibition by HCV-796 and boceprevir but had a reduced replicative capacity.
Quality of speech services in GSM-definition of a universal quality parameter for quality comparison and evaluation (2002)

Users of mobile communication networks tend to make higher demands upon quality of mobile communication services. Therefore it is important for GSM network operators to know the factors that determine the quality perceived by users of GSM speech services. Quality factors of GSM speech services are presented and a set of quality parameters belonging to these is given. Also a universal combined quality parameter, the quality of service index is proposed. This parameter eases quality comparison of different GSM network options and it enables objective quality evaluation in operational GSM networks.
Electron–channelling imaging in scanning electron microscopy (1979)

Abstract Electron–channelling imaging is a new method for observing defects in solid crystalline samples. The images are observed by scanning electron microscopy with backscattered electrons and have the same origin as the contrast observed in electron–channelling patterns. The observations reported here concern dislocations in silicon. The explored depth benoath the surface is around 1000 A and the resolution around 100 A.
Functional Analysis of Troponin I Regulatory Domains in the Intact Myofilament of Adult Single Cardiac Myocytes (1999)

Troponin I is the putative molecular switch for Ca(2+)-activated contraction within the myofilament of striated muscles. To gain insight into functional troponin I domain(s) in the context of the intact myofilament, adenovirus-mediated gene transfer was used to replace endogenous cardiac troponin I within the myofilaments of adult cardiac myocytes with the slow skeletal isoform or a chimera of the slow skeletal and cardiac isoforms. Efficient expression and myofilament incorporation were observed in myocytes with each exogenous troponin I protein without detected changes in the stoichiometry of other contractile proteins and/or sarcomere architecture. Contractile function studies in single, permeabilized myocytes expressing exogenous troponin I provided support for the presence of a Ca(2+)-sensitive regulatory domain in the carboxyl terminus of troponin I and a second, newly defined Ca(2+)-sensitive domain residing in the amino terminus of troponin I. Additional experiments demonstrated that the isoform-specific, acidic pH-induced contractile dysfunction in myocytes appears to lie in the carboxyl terminus of troponin I. Functional results obtained from adult cardiac myocytes expressing the chimera or isoforms of troponin I now define multiple troponin I regulatory domains operating in the intact myofilament and provide new insight into the Ca(2+)-sensitive properties of troponin I during contraction.
Chemoembolization with cisplatin-microcapsule. (1985)

Cisplatin (CDDP) was microcapsulated with ethylcellulose. These CDDP-microcapsules (CDDP-m. c.) were prepared based on the principles of coacervation, with modification. Sustained release of CDDP from the microcapsule, especially non-protein-bound CDDP which has antitumor activity, was clearly demonstrated by an invitro test. By a bioassay method, it was proved that biological activity of CDDP was not affected by the micro-encapsulation process here employed. When CDDP-m. c. were infused into the maxillary artery of patients with carcinoma of maxillary sinus or oral cavity, CDDP level in the circulating blood was significantly lower than those of patients administered non-encapsulated CDDP intravenously. However, significantly high CDDP concentration in tumor tissue was found in the patients treated with CDDP-m. c. These results suggest that selective arterial infusion of CDDP-m. c. could exerts an intensive topical antitumor effects through microinfarction effect and prolonged drug release with decreased systemic side effect.