gebhart/patrepeval huggingface dataset:
Epigenetic Silencing of IRF7 and/or IRF5 in Lung Cancer Cells Leads to Increased Sensitivity to Oncolytic Viruses (2011)

Defective IFN signaling results in loss of innate immunity and sensitizes cells to enhanced cytolytic killing after Vesticular Stomatitis Virus (VSV) infection. Examination of the innate immunity status of normal human bronchial epithelial cells Beas2B and 7 lung cancer cells revealed that the abrogation of IFN signaling in cancer cells is associated with greater sensitivity to VSV infection. The disruption of the IFN pathway in lung cancer cell lines and primary tumor tissues is caused by epigenetic silencing of critical interferon responsive transcription factors IRF7 and/or IRF5. Although 5-aza-29deoxycytidine treatment fails to reactivate IRF7 and IRF5 expression or protect cells from VSV infection, manipulating IFN signaling by altering IRF expression changes the viral susceptibility of these cells. Lung cancer cells can be partially protected from viral killing using IRF5+IRF7 overexpression, whereas IFN pathway disruption by transfection of siRNAs to IRF5+IRF7 increases cells' vulnerability to viral infection. Therefore, IRF5 and IRF7 are key transcription factors in IFN pathway that determine viral sensitivity of lung cancer cells; the epigenetically impaired IFN pathway in lung cancer tissues provides potential biomarkers for successful selective killing of cancer cells by oncolytic viral therapy.
Asymmetrically designed siRNAs and shRNAs enhance the strand specificity and efficacy in RNAi. (2007)

RNAi can mediate allele-specific silencing, and offers an attractive approach for treatment of human diseases caused by dominant, gain-of-function gene mutations. However, allele-specific silencing requires that the RNAi target the mutated region of the mRNA, limiting the choices of the target sequences. This often results in the use of a suboptimal siRNAs or shRNAs. Unfavorable strand asymmetry, which leads to the sense strand rather than the antisense strand to be incorporated into RNA-induced silencing complex (RISC), can cause poor RNAi efficacy. We systematically tested an approach that places mismatches at or near the 5' of the antisense strand to create favorable strand asymmetry. Here we show that this approach can enhance the RNAi efficacy of not only siRNAs but also shRNAs synthesized from genes, which can be placed in various viral vectors. Thus, this design of asymmetric shRNAs could be potentially used in silencing dominant, gain-of-function gene mutations for gene therapy.
Immunization against Leishmania Donovani: Glucan as an Adjuvant with Killed Promastigotes (1981)

Mice were immunized by a series of intravenous injections of formalin-killed Leishmania donovani promastigotes alone and combined with glucan, a beta 1,3 polyglucose derivative of baker's yeast. In three separate experiments animals were challenged with viable parasites on day 21, 40 or 80 after immunization. Mice which received dead parasites and glucan exhibited resistance against challenge up to 80 days after immunization. Animals which had been injected with glucan alone exhibited a lesser degree of resistance but injections of killed promastigotes alone conferred no measurable resistance against infection.
High-Frequency Modeling of the Long-Cable-Fed Induction Motor Drive System Using TLM Approach for Predicting Overvoltage Transients (2010)

Induction motor drive systems fed with cables are widely used in many industrial applications. Accurate prediction of motor terminal overvoltage, caused by impedance mismatch between the long cable and the motor, plays an important role for motor dielectric insulation and optimal design of dv/dt filters. In this paper, a novel modeling methodology for the investigation of long-cable-fed induction motor drive overvoltage is proposed. An improved high-frequency motor equivalent circuit model is developed to represent the motor high-frequency behavior for the time- and frequency-domain analyses. The motor equivalent circuit parameters for the differential mode (DM) and common mode (CM) are extracted based on the measurements. A high-frequency cable model based on improved high-order multiple- <formula formulatype="inline" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex Notation="TeX">$\pi$</tex></formula> sections is proposed. The cable model parameters are identified from the DM impedances in open circuit (OC) and short circuit (SC). To obtain a computationally efficient solution that could potentially be integrated with the motor drive controller, the system equations are discretized and solved using transmission-line modeling (TLM) approach. The proposed methodology is verified on an experimental 2.2-kW ABB motor drive benchmark system. The motor overvoltage transients predicted by the proposed model is in excellent agreement with the experimental results and represents a significant improvement compared with the conventional models.
A chimeric live attenuated vaccine against Japanese encephalitis (2004)

Japanese encephalitis is a disease of the CNS, endemic in Asia and Oceania. The disease is refractory to drug treatments and whilst the rural economies remain heavily dependent on agriculture, conditions for propagation of the disease will persist. Thus, there is a need for effective vaccines. Although some currently exist, they have their shortcomings. ChimeriVax™-JE (Acambis Inc.) is a chimeric, live attenuated vaccine which expresses protective Japanese encephalitis antigens and to date has proven to be safe, effective and well-tolerated in clinical trials. It therefore appears to be a cost-effective prophylactic vaccine against this debilitating disease.
ChemInform Abstract: Asymmetric Construction of Functionalized Bicyclic Imides via [3 + 2] Annulation of MBH Carbonates Catalyzed by Dipeptide‐Based Phosphines. (2012)

Abstract Due to the biological importance of the products, the annulation is investigated on a gram scale: it takes place without loss of reactivity and enantioselectivity.
Evaluation of Sex Differences in Embryonic Heart Rates (1987)

Adult female chickens are known to have higher heart rates than adult males. Previous evidence suggested that sex-related differences in heart rates exist in embryos. The objective of the present study was to determine if differences in heart rate can be used for sexing chickens during embryonic development. The effects of hyper and hypothermia also were evaluated. On Day 14 of incubation, Single Comb White Leghorn embryos were candled and three surface electrodes were placed on the eggshell. The electrodes were attached to an electrocardiograph. Heart rates were recorded from Days 15 to 20 of incubation. Female embryos exhibited higher mean heart rates than males (2 to 4 beats/min) for Days 15 to 19 of incubation. Average heart rates of male and female embryos decreased and were significantly different when exposed to hypothermia (26.1 C for 2 h) on Day 15 of incubation. Hyperthermia (39.4 C for 45 min) on Day 15 of incubation increased embryonic heart rates, but male and female heart rates did not differ significantly. Extensive overlap between male and female heart rates under normal and hypothermic conditions makes this technique an industrially impractical method for determining embryonic sex.
Anti-Candida Activity of Some of the Iranian Medicinal Plants (2009)

In this study, anti-Candida activities of extract and essential oil of nine Iranian folklore plants including, Satureja bachtiarica Bunge., Thymus daenensis
Future Electric Supply and Demand: An Overview (1987)

The future electric supply and demand picture is marked by uncertainty. Forecasts now assume electric demands will be reduced by load management, conservation, and customer-owned generation. Will they be right? Could demand growth outstrip available supply? What must be done to protect the reliability of our vitally important electric supply?
Antifouling Potential of Lubricious, Micro-engineered, PDMS Elastomers against Zoospores of the Green Fouling Alga<i>Ulva (Enteromorpha)</i> (2004)

The settlement and release of Ulva spores from chemically modified, micro-engineered surface topographies have been investigated using poly(dimethyl siloxane) elastomers (PDMSe) with varying additions of non-network forming poly(dimethyl siloxane) based oils. The topographic features were based on 5 microns wide pillars or ridges separated by 5, 10, or 20 microns wide channels. Pattern depths were 5 or 1.5 microns. Swimming spores showed no marked difference in settlement on smooth surfaces covered with excess PDMS oils. However, incorporation of oils significantly reduced settlement density on many of the surfaces with topographic features, in particular, the 5 microns wide and deep channels. Previous results, confirmed here, demonstrate preferences by the spores to settle in channels and against pillars with spatial dimensions of 5 microns, 10 microns and 20 microns. The combination of lubricity and pillars significantly reduced the number of attached spores compared to the control, smooth, unmodified PDMSe surfaces when exposed to turbulent flow in a flow channel. The results are discussed in relation to the energy needs for spores to adhere to various surface features and the concepts of ultrahydrophobic surfaces. A factorial, multi-level experimental design was analyzed and a 2nd order polynomial model was regressed for statistically significant effects and interactions to determine the magnitude and direction of influence on the spore density measurements between factor levels.
Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. (1994)

Yeast mutants that are defective in acidification of the lysosome-like vacuole are able to grow at pH 5.5, but not at pH 7. Here, we present evidence that endocytosis is required for this low pH-dependent growth and use this observation to develop a screen for mutants defective in endocytosis. By isolating mutants that cannot grow when they lack the 60-kD vacuolar ATPase subunit (encoded by the VAT2 gene), we isolated a number of vat2-synthetic lethal (Vsl-) mutant strains. Seven of the Vsl- mutants are defective in endocytosis. Four of these mutant strains (end8-1, end9-1, end10-1, and end11-1) show altered uptake of the endocytosed ligand, alpha-factor, and three (end12-1, end12-2, and end13-1) are probably defective in transfer of internalized material to the vacuole. Most of the mutations also confer a strong Ts- growth defect. The mutants defective in uptake of alpha-factor sort newly synthesized vacuolar proteins correctly, while those which may be defective in subsequent transport steps secrete at least a fraction of the newly synthesized soluble vacuolar proteins. The mutations that result in a defect in alpha-factor uptake are not allelic to any of the genes previously shown to encode endocytic functions.
High-Power, Long-Lifetime InGaN Multi-Quantum-Well-Structure Laser Diodes (1997)

The continuous-wave operation of InGaN multi-quantum-well structure laser diodes (LDs) was demonstrated at room temperature with a high output power of 50 mW, a high operating temperature of 100° C and a long lifetime of 300 hours. The characteristic temperature of the threshold current was as high as 170 K. The emission wavelength of the LDs was 416 nm.
Implication of Mammalian Ribosomal Protein S3 in the Processing of DNA Damage (1995)

A human apurinic/apyrimidinic endonuclease activity, called AP endonuclease I, is missing from or altered specifically in cells cultured from Xeroderma pigmentosum group-D individuals (XP-D cells) (Kuhnlein, U., Lee, B., Penhoet, E. E., and Linn, S. (1978) Nucleic Acids Res. 5,951-960). We have now observed that another nuclease activity, UV endonuclease III, is similarly not detected in XP-D cells and is inseparable from the AP endonuclease I activity. This activity preferentially cleaves the phosphodiester backbone of heavily ultraviolet-irradiated DNA at unknown lesions as well as at one of the phosphodiester bonds within a cyclobutane pyrimidine dimer. The nuclease activities have been purified from mouse cells to yield a peptide of M(r) = 32,000, whose sequence indicates identity with ribosomal protein S3. The nuclease activities all cross-react with immunopurified antibody directed against authentic rat ribosomal protein S3, and, upon expression in Escherichia coli of a cloned rat cDNA for ribosomal protein S3, each of the activities was recovered and was indistinguishable from those of the mammalian UV endonuclease III. Moreover, the protein expressed in E. coli and its activities cross-react with the rat protein antibody. Ribosomal protein S3 contains a potential nuclear localization signal, and the protein isolated as a nuclease also has a glycosylation pattern consistent with a nuclear localization as determined by lectin binding. The unexpected role of a ribosomal protein in DNA damage processing and the unexplained inability to detect the nuclease activities in extracts from XP-D cells are discussed.
CFO tracking for Direct RF Sampling architecture applied to VHF avionic radios (2017)

Recently, many efforts have been made to respond to the increasing demands of a new RF avionic generation, in which Direct RF Sampling (DRFS) has been considered as a promising solution to satisfy the Size, Weight, Power and Cost (SWaPC) constraints. In this architecture, Direct Digital Synthesizer (DDS) plays a key role, significantly affecting the overall performance of the whole system. Theoretically, the output of DDS should have alias frequency of the Signal of Interest (SOI) in the first Nyquist zone. However, due to the resolution of the tuning word and the difference in frequency of the transmitter from the assigned frequency, this signal might differ from the target carrier, resulting in an inaccuracy shift of the digital mixer. Furthermore, this issue will distort the output signal and deteriorate the performance of the receiver. The work presented in this paper introduces an approach to solve this problem. Based on the conventional Automatic Frequency Control (AFC), the configurable Fast Fourier Transformation (FFT) based Carrier Frequency Offset (CFO) Tracking System has been designed and implemented to meet the new challenges of DRFS in avionic receiver design. The experimental results show that the integration of this module increases the sensitivity of the DRFS architecture for avionic applications, such as VHF Omnidirectional Range (VOR), Instrument Landing System (ILS) and VHF audio communication.
Study of vancomycin susceptibility in methicillin-resistant Staphylococcus aureus isolated from clinical samples (2012)

Background : Staphylococcus aureus is one of the most commonly isolated organisms in nosocomial infections. While the prevalence of methicillin--resistant Staphylococcus aureus (MRSA) continues to increase worldwide, there is a concern about an increase in vancomycin minimum inhibitory concentrations (MIC,s) among S. aureus strains. An attempt was, therefore, made to study vancomycin susceptibility in 156 MRSA isolates from various clinical samples during the period of February 2008 to January 2009. Materials and Methods: A total of 156 isolates of S. aureus were collected from various clinical specimens. MIC of vancomycin was detected by agar dilution method. Results : Out of 156 MRSA isolates all were susceptible to vancomycin by disc diffusion method. By agar dilution method, 138 isolates were susceptible to vancomycin (VISA MIC 0.5-2 μgm/ml) and 18 isolates showed intermediate susceptibility to vancomycin (VISA MIC 4-8 μgm/ml). No vancomycin resistant S. aureus (VRSA) with MIC ≥ 16 μgm/ml was detected. Conclusion : The present study reveals the emergence of MRSA with reduced susceptibility to vancomycin and indicates the magnitude of antibiotic resistance in and around Davangere.
Three Abnormal Fibrinogen Variants with the Same Amino Acid Substitution (γ 275 Arg → His): Fibrinogens Bergamo II, Essen and Perugia (1986)

We report on three unrelated individuals with the same uncommon type of dysfibrinogenemia, originating from Bergamo, Essen and Perugia. None of them showed bleeding symptoms while the Bergamo patient and members of her family presented with a thrombotic tendency. The presence of a defective fibrinogen was suggested by prolonged thrombin and reptilase times. Furthermore, fibrinogen concentrations of less than 0.28 g/L were determined by the functional assay whereas values of 1.5-2.4 g/L were measured by heat precipitation or electroimmunoassay. Fibrinogen was isolated by affinity chromatography on insoluble fibrin monomer. The rate of fibrinopeptide release by thrombin was normal while the fibrin polymerization reaction was strongly delayed. An abnormal peptide (gamma 265-310) was isolated by high-performance liquid chromatography after cyanogen bromide cleavage of the purified gamma-chain of fibrinogen Bergamo II and Essen. The same peptide was also isolated following cyanogen bromide treatment of the intact fibrinogen Perugia. Sequence analyses of these peptides demonstrated the same amino acid exchange in all three fibrinogens: gamma 275 arginine----histidine. The described fibrinogen variants appear to possess a molecular defect which has thus far only been observed in fibrinogen Haifa.
Out-of-focus blur estimation and restoration fordigital auto-focusingsystem (1998)

A fully digital auto-focusing system based on point-spread function (PSF) estimation is proposed. To increase accuracy in estimating the PSF of the defocused image, the input image is subdivided, and the step responses of edge-containing subimages are averaged. The PSF, which results from out-of-focus blur, is estimated by differentiating the averaged step response along the appropriately estimated edge direction under the assumption that an image usually contains a piece-wise linear boundary between an object and background. By using the estimated PSF, the corresponding image restoration filter is realised, based on the constrained least squares approach.
Scene-Based Nonuniformity Correction with Reduced Ghosting Using a Gated LMS Algorithm (2009)

In this paper, we present a scene-based nouniformity correction (NUC) method using a modified adaptive least mean square (LMS) algorithm with a novel gating operation on the updates. The gating is designed to significantly reduce ghosting artifacts produced by many scene-based NUC algorithms by halting updates when temporal variation is lacking. We define the algorithm and present a number of experimental results to demonstrate the efficacy of the proposed method in comparison to several previously published methods including other LMS and constant statistics based methods. The experimental results include simulated imagery and a real infrared image sequence. We show that the proposed method significantly reduces ghosting artifacts, but has a slightly longer convergence time.
Cotransport of water by the Na <sup>+</sup> /glucose cotransporter (1996)

Water is transported across epithelial membranes in the absence of any hydrostatic or osmotic gradients. A prime example is the small intestine, where 10 liters of water are absorbed each day. Although water absorption is secondary to active solute transport, the coupling mechanism between solute and water flow is not understood. We have tested the hypothesis that water transport is directly linked to solute transport by cotransport proteins such as the brush border Na+/glucose cotransporter. The Na+/glucose cotransporter was expressed in Xenopus oocytes, and the changes in cell volume were measured under sugar-transporting and nontransporting conditions. We demonstrate that 260 water molecules are directly coupled to each sugar molecule transported and estimate that in the human intestine this accounts for 5 liters of water absorption per day. Other animal and plant cotransporters such as the Na+/CI-/gamma-aminobutyric acid, Na+/iodide and H+/amino acid transporters are also able to transport water and this suggests that cotransporters play an important role in water homeostasis.
T-cell apoptosis in situ in experimental autoimmune encephalomyelitis following methylprednisolone pulse therapy (2000)

The apoptosis-inducing effects of i.v. methylprednisolone were investigated as a possible method of controlling inflammation in the CNS in adoptive transfer-experimental autoimmune encephalomyelitis (AT-EAE) in Lewis rats. Two pulses of methylprednisolone were given at the peak of mild and of severe disease. T-cell apoptosis was assessed on spinal cord cross-sections by morphology and TUNEL staining. Concentrations of methylprednisolone were measured in serum, CSF and spinal cord tissue by high-pressure liquid chromatography (HPLC). In severe EAE, 10 mg/kg methylprednisolone increased T-cell apoptosis significantly and T-cell infiltration was marginally decreased. A maximal dose of 50 mg/kg methylprednisolone was superior in both respects and, in contrast to 10 mg/kg methylprednisolone, was also effective in mild EAE. A dose of 1 mg/kg methylprednisolone did not produce notable changes compared with controls treated with phosphate-buffered saline. Serum, CSF and spinal cord concentrations of methylprednisolone measured by HPLC 2 h after a single i.v. injection of 10 or 50 mg/kg methylprednisolone revealed significantly higher methylprednisolone concentrations in severe EAE compared with mild disease. With 50 mg/kg methylprednisolone, we obtained serum and CSF concentrations in the region of 10(-5) M methylprednisolone. We also studied the expression of bcl-2, a typical anti-apoptotic regulatory protein, in T cells, and found no change after methylprednisolone treatment compared with controls. Methylprednisolone did not induce apoptosis of oligodendrocytes, which would have been an unwanted side effect in CNS cells. This study provides evidence that methylprednisolone dose-dependently augments T-cell apoptosisin situ in AT-EAE. Our results may have implications for the use of glucocorticosteroids at very high doses in the treatment of inflammatory disorders of the CNS, such as multiple sclerosis, or of other target organs.
Pharmacological treatment of erectile dysfunction (1999)

There is growing evidence that the field of pharmacotherapy, particularly oral drugs, will be dominant in the future management of sexual dysfunction. Basic research has led to the understanding of the intracellular mechanisms that control penile smooth muscle contractility and therefore erection, opening a vast area for pharmacological intervention. Moreover, the importance of central neurohormonal mechanisms has made these pathways the target for new centrally acting drugs. Given these trends most patients suffering from erectile dysfunction will respond to pharmacological agents in the not so distant future.