gebhart/patrepeval huggingface dataset:
Functional Role of Lanthanides in Enzymatic Activity and Transcriptional Regulation of Pyrroloquinoline Quinone-Dependent Alcohol Dehydrogenases in <i>Pseudomonas putida</i> KT2440 (2017)

The oxidation of alcohols and aldehydes is crucial for detoxification and efficient catabolism of various volatile organic compounds (VOCs). Thus, many Gram-negative bacteria have evolved periplasmic oxidation systems based on pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs) that are often functionally redundant. Here we report the first description and characterization of a lanthanide-dependent PQQ-ADH (PedH) in a nonmethylotrophic bacterium based on the use of purified enzymes from the soil-dwelling model organism <i>Pseudomonas putida</i> KT2440. PedH (PP_2679) exhibits enzyme activity on a range of substrates similar to that of its Ca<sup>2+</sup>-dependent counterpart PedE (PP_2674), including linear and aromatic primary and secondary alcohols, as well as aldehydes, but only in the presence of lanthanide ions, including La<sup>3+</sup>, Ce<sup>3+</sup>, Pr<sup>3+</sup>, Sm<sup>3+</sup>, or Nd<sup>3+</sup> Reporter assays revealed that PedH not only has a catalytic function but is also involved in the transcriptional regulation of <i>pedE</i> and <i>pedH</i>, most likely acting as a sensory module. Notably, the underlying regulatory network is responsive to as little as 1 to 10 nM lanthanum, a concentration assumed to be of ecological relevance. The present study further demonstrates that the PQQ-dependent oxidation system is crucial for efficient growth with a variety of volatile alcohols. From these results, we conclude that functional redundancy and inverse regulation of PedE and PedH represent an adaptive strategy of <i>P. putida</i> KT2440 to optimize growth with volatile alcohols in response to the availability of different lanthanides.<b>IMPORTANCE</b> Because of their low bioavailability, lanthanides have long been considered biologically inert. In recent years, however, the identification of lanthanides as a cofactor in methylotrophic bacteria has attracted tremendous interest among various biological fields. The present study reveals that one of the two PQQ-ADHs produced by the model organism <i>P. putida</i> KT2440 also utilizes lanthanides as a cofactor, thus expanding the scope of lanthanide-employing bacteria beyond the methylotrophs. Similar to the system described in methylotrophic bacteria, a complex regulatory network is involved in lanthanide-responsive switching between the two PQQ-ADHs encoded by <i>P. putida</i> KT2440. We further show that the functional production of at least one of the enzymes is crucial for efficient growth with several volatile alcohols. Overall, our study provides a novel understanding of the redundancy of PQQ-ADHs observed in many organisms and further highlights the importance of lanthanides for bacterial metabolism, particularly in soil environments.
Rheology of Liquid Crystalline Elastomers in Their Isotropic and Smectic A State (1999)

The dynamics of two different side-chain liquid crystalline elastomers (SCLCE) exhibiting a smectic A phase is investigated by low-frequency shear and compression experiments. We find that both the isotropic and the smectic A phase have their own characteristic viscoelastic behavior, which seems to be independent of the compound under investigation and thus universal. The relaxation in the isotropic phase shows a distribution of relaxation times, which gives rise to a scaling law of the elastic moduli with an exponent of 0.5. The viscoelastic response in the smectic A phase, however, exhibits a much broader spectrum of relaxation times containing very long-lived modes. It appears that there exists a low-frequency scaling law with an exponent of 0.3 characterizing the smectic A phase. We propose that this result stems from a transient smectic network with a very long lifetime that was set up by the smectic domains. At the phase transition, this transient smectic network disappears, which leads to a sharp decrease of the dynamic shear as well as the compression modulus.
Characterization of the Metal Phase in NM/Ce<sub>0.68</sub>Zr<sub>0.32</sub>O<sub>2</sub> (NM:  Pt and Pd) Catalysts by Hydrogen Chemisorption and HRTEM Microscopy:  A Comparative Study (2001)

Pt and Pd particles supported on a texturally stable Ce0.68Zr0.32O2 mixed oxide were investigated by means of H2 chemisorption and high-resolution transmission electron microscopy (HRTEM). The comparison of the metal dispersion data as determined by HRTEM with those estimated from H2 volumetric adsorption reveals that, in the case of Pt catalysts reduced at temperatures below 350 °C, the chemisorption isotherms recorded at −80 °C provide reliable dispersion data. For catalysts reduced at, or above, 350 °C, some platinum deactivation occurred. However, H2 chemisorption capability was recovered by reoxidation at 427 °C and further reduction at 150 °C. For catalysts reduced at 700 °C or 900 °C, the recovery of the platinum chemisorptive capability was only partial, even if a more severe reoxidation treatment (700 °C) was applied. To prevent hydride formation, H2 chemisorption on the Pd/Ce0.68Zr0.32O2 was investigated at low H2 pressures and at room temperature. Under these experimental conditions, which are often employed for characterizing Pd catalysts, hydrogen spillover cannot be blocked, thus preventing a reliable estimate of the metal dispersion in our ceria−zirconia -supported palladium catalysts.
Production planning in integrated maintenance context for multi-period multi-product failure-prone single-machine (2013)

In this paper, a multi-period, multi-product single randomly failing and repairable machine under a combined approach of maintenance management and production control is studied. The production unit aims to satisfy the deterministic market demands with variable production rates for non-homogeneous delivery periodicities without postponement or backlog. We consider production cost depends on the using rate of machine. Also, we consider a production rate dependent failure rate. The aim of this study is to determine the optimal production rate and the production period duration for each product order those minimizing the expected total cost, including holding, shortage, production, setup and maintenance costs. We propose a mathematical model and we develop a genetic algorithm based optimization procedure. We discuss and conduct computational experiments to study the managerial insights for the developed strategy.
PACS sans RIS. (2004)

Sutter Gould Medical Foundation (SGMF) is a multi-specialty physician practice located in Modesto, CA, and is an affiliate of Sutter Health. In May 2003, SGMF embarked on the journey to install a picture archiving and communication system (PACS) in the main facility. Unique to the project was bringing the PACS live sans (from the French "without") a radiology information system (RIS) and having the PACS driven by an electronic medical record (EMR). Other challenges were present in terms of interfaces for the dictation system, delivering images to the providers on their desktop, and mimicking the referring providers workflow as much as possible in the digital environment to which they were accustomed in the "paper and film" environment.
Simultaneous Localization and Mapping for Forest Harvesters (2007)

A real-time SLAM (simultaneous localization and mapping) approach to harvester localization and tree map generation in forest environments is presented in this paper. The method combines 2D laser localization and mapping with GPS information to form global tree maps. Building an incremental map while also using it for localization is the only way a mobile robot can navigate in large outdoor environments. Until recently SLAM has only been confined to small-scale, mostly indoor, environments. We try to addresses the issues of scale for practical implementations of SLAM in extensive outdoor environments. Presented algorithms are tested in real outdoor environments using an all-terrain vehicle equipped with the navigation sensors and a DGPS receiver.
A 95%-Efficient 48V-to-1V/10A VRM Hybrid Converter Using Interleaved Dual Inductors (2018)

This paper presents a new 48 V-to-1 V hybrid converter. The converter utilizes two interleaved inductors to achieve complete soft-charging of flying capacitors to efficiently support high output currents. This dual inductor hybrid converter (DIHC) features fewer number of switches and more effective switch utilization than a recently reported hybrid Dickson converter, leading to substantially less conduction losses presented by a smaller equivalent output impedance. Experimental results verify the converter's operation principles and advantages in a 300-kHz 20-W prototype achieving 95.02% peak efficiency and 225 W/in3 power density. Its advantages and performance promise a good candidate converter architecture for applications that require large conversion ratios and high output currents, such as data centers and high-performance digital systems.
Photoactivity of Cinnamate-Intercalates of Layered Double Hydroxides (1992)

Abstract The synthesis of photoactive (cinnamate) intercalates of layered double hydroxides is described. Corresponding single-crystal data on pure sodium salts enables information concerning the arrangement of the guest molecules within the hydroxide to be obtained.
Genetic correction of canine dystrophic epidermolysis bullosa mediated by retroviral vectors (2003)

We have assessed the suitability of retroviral vectors for gene therapy of recessive dystrophic epidermolysis bullosa (RDEB) in dogs expressing a mutated collagen type VII. Isolation and analysis of the 9 kb dog collagen type VII cDNA identified the causative genetic mutation G1906S and disclosed the interspecies conservation of collagen type VII. Highly efficient transfer of the wild-type collagen type VII cDNA to both dog RDEB and human primary RDEB collagen type VII-null keratinocytes using recombinant vectors derived from LZRS-Ires-zeo and MSCV retroviruses achieved sustained and permanent expression of the transgene product. The expression and post-translational modification profile of the recombinant collagen type VII was comparable to that of the wild-type counterpart. The recombinant canine collagen type VII in human RDEB keratinocytes and dog cells corrected the observable defects caused by RDEB keratinocytes in cell cultures and in vitro reconstructed skin. Hypermotility was fully reverted in human RDEB keratinocytes, and strongly reduced in the dog RDEB cells. This observation suggests that not only infection efficiency but also high expression levels are required to ensure therapeutic efficacy in the presence of mutated gene products. Our results set the basis for preclinical gene therapy assays in the first immune-competent large animal model for an inherited skin disease and broaden the spectrum of preclinical and clinical applications of retroviral vectors in the transfer of large recombinant genes in epithelial cells.
Nucleoplasmic lamins and their interaction partners, LAP2α, Rb, and BAF, in transcriptional regulation (2007)

Lamins are major structural components of the nuclear envelope in multicellular eukaryotes. Particularly A-type lamins are also located in the nucleoplasm, likely involving a specific binding partner, lamina-associated polypeptide 2alpha (LAP2alpha). LAP2alpha-lamins A/C complexes in the nucleoplasm have been implicated in the regulation of gene expression by various means. They bind chromatin proteins and chromatin modifying enzymes, and can thus participate in epigenetic control pathways. Furthermore, binding of lamins A/C complexes to specific transcription factors and repressors may directly affect their transcriptional activity. LAP2alpha-lamins A/C also regulate retinoblastoma protein and influence cell cycle progression and differentiation, which could have important implications for molecular mechanisms of laminopathic diseases, linked to lamins A/C mutations.
Suppressor T cells prevent experimental autoimmune encephalomyelitis in mice. (1977)

Immune suppression (immunoprotection) in experimental autoimmune encephalomyelitis (EAE) was studied in (SJL X BALB/c)F1 mice using inocular of mouse spinal cord homogenate (MSCH), or mouse basic protein of myelin (M-BPM), in Freund's incomplete adjuvant (FIA). Such immunization specifically recruited lymphoid cells which markedly suppressed the capacity of effector lymph node cells from appropriately immunized syngeneic mice to transfer adoptively EAE. Suppression was demonstrable with transfer of bone marrow and spleen cells, but not with lymph nodes or thymus cells. Adoptively transferred suppression was maximal when cells were injected 9-30 days after the suppressive injection. Inhibition of EAE by suppressor cells was specific for the relavant antigen BPM, and required viable cells. Treatment of cells with anti-Thy-1 serum before transfer abolished their suppressor activity. After adoptive transfer of suppressor cells into syngeneic recipients subsequently immunized for EAE, there was inhibition of EAE and reduced cell-mediated immune response to BPM as judged by macrophage migration inhibition assays. Hence, in mice at least, immuno-protection against EAE is explicable by recruitment of suppressor T lymphocytes with the dual capacities of inhibiting development of effector T cells after antigenic stimulation, and of blocking their damaging effects on the antigen in the central nervous system.
Mistranslation of the mRNA encoding bacteriophage T7 0.3 protein. (1984)

We have devised an experimental system using the T7 phage 0.3 protein to accurately quantitate in vivo errors in protein synthesis. The 0.3 protein is well suited for mistranslation studies because it is easy to purify, its precise amino acid and RNA sequences are known, and it contains no cysteine. Utilizing [35S]cysteine as a precursor we found an average of 1 cysteine residue misincorporated for every 43.5 molecules of 0.3 protein synthesized. Since there are 116 amino acids in 0.3 protein, 1 cysteine residue was misincorporated /5000 codons translated. If all 20 amino acids were misincorporated at the same frequency, the overall frequency of misincorporation of amino acids into 0.3 protein would be 4 X 10(-3)/codon translated. Parallel experiments measuring [35S]methionine incorporation into 0.3 protein supported the accuracy of our findings for cysteine misincorporation. We found an average of 5.7 methionine residues incorporated/molecule of 0.3 protein synthesized; the actual number from sequence data is known to be 6. Antibiotics which stimulate mistranslation (gentamicin and streptomycin) caused a modest increase in the number of cysteine residues misincorporated into 0.3 protein. The use of Escherichia coli strains, identical except for mutations in ribosomal protein genes known to affect the fidelity of translation, supported the contention that the errors being quantitated were mainly due to mistranslation rather than mistranscription .
Processing optimization of solution derived PbZr<sub>1−x</sub>Ti<sub>x</sub>O<sub>3</sub> thin films for piezoelectric applications (2001)

Abstract Processing optimization allowed the sol-gel fabrication of 1 μm thick, phase pure perovskite thin films with identical grain size and controlled texture. This made it possible to fabricate stress compensated beams to measure the transverse piezoelectric coefficient over the whole composition range of PbZr1−xTixO3. The highest value of -12.1 C/m2 was measured for (100)/(001) textured PZT53/47. For (111) textured films the maximum value of -8.7 C/m2 was found to be in the tetragonal phase field at 55% Ti.
High Organochlorine Body Burden in Women With Estrogen Receptor-Positive Breast Cancer (1994)

Journal Article High Organochlorine Body Burden in Women With Estrogen Receptor-Positive Breast Cancer Get access Éric Dewailly, Éric Dewailly Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Sylvie Dodin, Sylvie Dodin Hopital Saint-Francois d'Assise Research Center, Quebec Search for other works by this author on: Oxford Academic PubMed Google Scholar René Verreault, René Verreault Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Pierre Ayotte, Pierre Ayotte Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Louise Sauvé, Louise Sauvé Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada Search for other works by this author on: Oxford Academic PubMed Google Scholar Jacques Morin, Jacques Morin Hopital Saint-Francois d'Assise Research Center, Quebec Search for other works by this author on: Oxford Academic PubMed Google Scholar Jacques Brisson Jacques Brisson * Department of Social and Preventive Medicine, Faculty of Medicine, Laval University, Quebec, Canada * Correspondence to: Eric Dewailly, Department of Social and Preventive Medicine, faculty of Mdeicine. Laval University, G1K 7P4. Quebed, Canada. Search for other works by this author on: Oxford Academic PubMed Google Scholar JNCI: Journal of the National Cancer Institute, Volume 86, Issue 3, 2 February 1994, Pages 232–234, https://doi.org/10.1093/jnci/86.3.232 Published: 02 February 1994 Article history Received: 17 August 1993 Revision received: 03 November 1993 Accepted: 09 November 1993 Published: 02 February 1994
Consensus for Crosschain Communications (2020)

Crosschain communications allows information to be communicated between blockchains. Consensus, in the context of crosschain communications, relates to how participants are able to agree on the state of one blockchain and communicate that information to another blockchain such that the information is trusted. This chapter reviews the usage scenarios of crosschain communications: value transfer and atomic swaps, reading, writing, and state pinning. It analyses what attributes must be maintained for permissionless and permissioned blockchains using a crosschain consensus mechanism such that the properties of each blockchain are not compromised. Finally, this chapter reviews the main categories of crosschain communications techniques: Hash Time Locked Contracts, block header relaying (BTC Relay, Pegged Sidechains and Ion), relay chains (Polkadot and Cosmos), and coordination chains with threshold schemes (Atomic Crosschain Transaction).
In Ga N ∕ Ga N light emitting diodes on nanoscale silicon on insulator (2007)

The authors report on the fabrication of InGaN∕GaN-based light emitting diodes (LEDs) on nanoscale silicon-on-insulator (SOI) substrates. The LED structures are grown on (111)-oriented 45nm thick SOI overlayer by metal organic chemical vapor deposition. Square-shaped mesa patterns are created by standard LED processing steps including multiple-mask photolithography, inductive coupled plasma etching, and contact metallization. Due to the high reflective Si∕SiO2 beneath AlN buffer and high refractive contrasts at the interfaces, the authors observed multiple interference peaks from LEDs on SOI and such effect resulted in an increased integrated electroluminescence intensity when compared to LED structures fabricated on bulk Si(111).
제 8 회 학술발표대회 발표 논문 / 한자병용에 따른 한자한문교육의 방향 : 대학에서의 한문교육 현황과 개선방안 (2001)

Nowadays Korean society including colleges and universities is paying great attention to teaching such practical subjects as English and Computer under the banner of internalization or globalization. In this stream of the modern age Chinese (literacy) programs are losing their ground day after day. Hence the author diagnosed the present status and problems of Chinese education executed in two colleges to locate the correct place of college Chinese education and to come up with the outline of the lessons that the students really want to take. A survey showed that 90 percent of the students agreed to the necessity of Chinese education, that their own knowledge of Chinese were limited, and that they were making efforts to improve their proficiency through individual study and by attending the classes offered. However, their basic interest in and knowledge of Chinese didn`t go beyond knowing the names of the works that were printed in the highschool textbooks, and they showed much interest in currently used characters or the old phraseology rather than the sentences. Some question items of the survey revealed that the students wanted to learn as much Chinese as they could read the newspapers. They simply want to learn words of Chinese characters, not the sentences from which we can discover the ideal or the view of value of our ancestors. This means that the present curriculum of Chinese education which focuses on sentence reading doesn`t meet the students` needs. In this respect, the author has thought about the lessons or materials that will fit into the stream of the age and meet the challenges. First of all we offer a curriculum with special focus on idiomatic Chinese and lead the students in the classroom to work on currently used Chinese words, not the sentences from the past literature. Second, we utilize the format of a special lecture series such as English TOEFL instead of offering a regular course. Third, we let the students have a broad choice toward Chinese literacy by offering subdivided courses like Chinese Novel, Chinese Poetry, Old Phraseology, Confucian Bible, and so on. We teachers should never forget that the fast-changing society and the new generation of students require us to adapt our Chinese education in colleges to the new stream and to the new tastes.
Two-photon-induced photochromic reactions in spirobenzopyran-doped poly(methyl methacrylate) thin-film waveguides (1996)

We present experimental results of photochromic reactions induced by single- and two-photon excitations in a poly(methyl methacrylate) thin film doped with spirobenzopyran. We also demonstrate the operation of a spirobenzopyran-doped poly(methyl methacrylate) planar waveguide, prepared by cast spinning the media onto fused-quartz slides. The following phenomena were shown to take place in this waveguide: single-twophoton coloring, f luorescing, and bleaching. The results indicate that such devices may be suitable for many applications, including optical waveguides, volume optical storage, and optical sensors.
Ultra-low power nonlinear optics in a high Q CMOS compatible integrated micro-ring resonator (2017)

We demonstrate efficient, low power, continuous-wave four-wave mixing in the C-band, using a high index doped silica glass micro ring resonator having a Q-factor of 1.2 million. A record high conversion efficiency for this kind of device is achieved over a bandwidth of 20nm. We show theoretically that the characteristic low dispersion enables phase-matching over a bandwidth > 160nm.