gebhart/patrepeval huggingface dataset:
Acrolein Application To Mitigate Biogenic Sulfides and Remediate Injection-Well Damage in a Gas-Plant Water-Disposal System (2006)

Abstract The unique and reactive chemistry of acrolein enables a 3-fold approach to oilfield problems stemming from sulfate reducing bacteria (SRB): 1) it is an effective biocide, 2) it scavenges H2S, and 3) it dissolves iron sulfide. In this study, the utility of acrolein for remediation of problems with SRB and biogenic sulfides in a gas plant water disposal system at an facility near Mobile, Alabama is presented. The disposal system, designed to handle produced water from offshore, process water from gas plant operations, and surface water drainage within the plant, was losing capacity due to iron sulfide fouling of the surface equipment and plugging of the disposal wells. Bacterial monitoring indicated that SRB originating from the drain water had become established in the tanks and flowlines. These SRB were generating 8-10 ppm dissolved H2S that was ultimately responsible for the iron sulfide formation. The iron sulfide resulted in poor water quality (Millipore® rates = 200-300 ml/5 min), tank fouling, filter plugging, injection line scaling, and loss of injectivity due to near well bore damage in the disposal wells. The treatment design consisted of 1) initial remediation of injection wells by acrolein squeeze stimulations using a coiled tubing unit, 2) removal of iron sulfide pads from the tankage and iron sulfide build up in the disposal lines, and mitigation of SRB populations via batch treatments with acrolein, and 3) preventive maintenance using a continuous and batch treatment program with acrolein. Immediately following squeeze treatment of the disposal wells, a 30% increase in capacity was observed. The topside treatment program has resulted in a 400 to 500% improvement in Millipore® rates, decreased levels of SRB, greater than 90% decrease in soluble H2S and a 65% decrease in filter replacements. Overall benefits of the program include acceptable injection rates and pressures and reduced operating costs through decreased filter changes, remedial expenses to maintain the disposal wells, and water trucking expenses.
tRNA-Derived Fragments (tRFs): Emerging New Roles for an Ancient RNA in the Regulation of Gene Expression (2015)

This review will summarise the recent discoveries and current state of research on short noncoding RNAs derived from tRNAs-known as tRNA-derived fragments (tRFs). It will describe the features of the known subtypes of these RNAs; including sequence characteristics, protein interactors, expression characteristics, biogenesis, and similarity to canonical miRNA pathways. Also their role in regulating gene expression; including mediating translational suppression, will be discussed. We also highlight their potential use as biomarkers, functions in gene regulation and links to disease. Finally, this review will speculate as to the origin and rationale for the conservation of this novel class of noncoding RNAs amongst both prokaryotes and eukaryotes.
The Clinical Significance of the Dense Fine Speckled Immunofluorescence Pattern on HEp-2 Cells for the Diagnosis of Systemic Autoimmune Diseases (2012)

Antinuclear antibodies (ANAs) are a serological hallmark in the diagnosis of systemic autoimmune rheumatic diseases (SARD). The indirect immunofluorescence (IIF) assay on HEp-2 cells is a commonly used test for the detection of ANA and has been recently recommended as the screening test of choice by a task force of the American College of Rheumatology. However, up to 20% of apparently healthy individuals (HI) have been reported to have a positive IIF ANA test, primarily related to autoantibodies that target the dense fine speckles 70 (DFS70) antigen. Even more important, the DFS IIF pattern has been reported in up to 33% of ANA positive HI, but not in ANA positive SARD sera. Since the intended use of the ANA HEp-2 test is to aid in the diagnosis and classification of SARD, the detection and reporting of anti-DFS70 antibodies and their associated pattern (DFS) as a positive test significantly reduce the specificity and the positive likelihood of the ANA test. This has significant implications for medical management and diagnostic algorithms involving the detection of ANA. Recently, a novel immunoadsorption method has been developed that specifically blocks anti-DFS70 antibodies and, therefore, significantly increases the specificity of the ANA test for SARD. This immunoadsorption method has the potential to overcome a significant limitation of the ANA HEp-2 assay. The present paper summarizes the current knowledge about anti-DFS70 antibodies and their clinical impact on ANA testing.
Facile One-Pot Synthesis of α-Chloro Sulfoxides from Sulfides (1993)

Various sulfides 1 reacted with sulfuryl chloride in the presence of metal nitrates in acetonitrile under mild conditions to give the corresponding α-chloro sulfoxides 2 in good yields.
True time-delay phased-array antenna feed system based on optical heterodyne techniques (1996)

We demonstrate a true-time delay phased-array antenna feed system based on multi-channel optical heterodyne detection techniques. The system is relatively insensitive to laser linewidth and IF frequency instabilities. We demonstrate accurate true-time delay from 0.8 to 1.5 GHz. The dynamic range for one channel is 52 dB for 1 MHz bandwidth. For a very narrow channel spacing of 13 GHz (/spl sim/1 /spl Aring/ at a transmission wavelength of 1.55 μm), the interchannel interference is negligible. Such an architecture has potential for large scale system implementations.
Clean Catheterization (1951)

MBRxISOH 529 no further Herxheimer reaction was noted and that the temperature remained normal and the pulse at 80 or below.
Intelligent telemonitoring of critical-care patients (1999)

Sutil+ is an intelligent monitoring system that is under development. The aim is to endow the system with more intelligent behaviour and to make the presentation of information to the user as flexible as possible, in space, time, and form. We present two telemonitoring solutions that enable access to information resulting from the monitoring of patients in coronary care units, independent of the location of the system user. We concentrate on aspects of data acquisition and storage and, above all, interaction with the user.
Efficient internet traffic delivery over wireless networks (2003)

The high demand for wireless Internet connectivity has driven the development of highly efficient radio link technologies. However, their performance can be compromised by inadvertent interactions with the higher-layer TCP flow control protocol. Maximizing the performance of wireless links requires that mechanisms operating at every layer of the protocol stack interact efficiently. This article provides a brief tutorial of some of these radio link enhancements. It then outlines how higher-layer flow control protocols should behave, and provides techniques for taming the behavior of TCP, to ensure that the performance of lower-layer enhancements is not compromised.
Diverse Origin and Function of Cells With Endothelial Phenotype Obtained From Adult Human Blood (2003)

Cells with endothelial phenotype generated from adult peripheral blood have emerging diagnostic and therapeutic potential. This study examined the lineage relationship between, and angiogenic function of, early endothelial progenitor cells (EPCs) and late outgrowth endothelial cells (OECs) in culture. Culture conditions were established to support the generation of both EPCs and OECs from the same starting population of peripheral blood mononuclear cells (PBMCs). Utilizing differences in expression of the surface endotoxin receptor CD14, it was determined that the vast majority of EPCs arose from a CD14+ subpopulation of PBMCs but OECs developed exclusively from the CD14- fraction. Human OECs, but not EPCs, expressed key regulatory proteins endothelial nitric oxide synthase (eNOS) and caveolin-1. Moreover, OECs exhibited a markedly greater capacity for capillary morphogenesis in in vitro and in vivo matrigel models, tube formation by OECs being in part dependent on eNOS function. Collectively, these data indicate lineage and functional heterogeneity in the population of circulating cells capable of assuming an endothelial phenotype and provide rationale for the investigation of new cell-therapeutic approaches to ischemic cardiovascular disease.
Advances in metabolic engineering of yeast<i>Saccharomyces cerevisiae</i>for production of chemicals (2014)

Yeast Saccharomyces cerevisiae is an important industrial host for production of enzymes, pharmaceutical and nutraceutical ingredients and recently also commodity chemicals and biofuels. Here, we review the advances in modeling and synthetic biology tools and how these tools can speed up the development of yeast cell factories. We also present an overview of metabolic engineering strategies for developing yeast strains for production of polymer monomers: lactic, succinic, and cis,cis-muconic acids. S. cerevisiae has already firmly established itself as a cell factory in industrial biotechnology and the advances in yeast strain engineering will stimulate development of novel yeast-based processes for chemicals production.
Wide Operational Speed Range of Five-Phase Permanent Magnet Machines by Using Different Stator Winding Configurations (2011)

The winding changeover technique is an effective method for extending the operational speed range of permanent magnet synchronous machines (PMSMs). Unlike three-phase machines which could be wound in wye or delta connections, there are three possible connections, i.e., star, pentagon, and pentacle for five-phase electric machines. In this work, the effects of these three winding connections on a five-phase PMSM performance are studied. First, based on the mathematical model and finite-element analysis, the effects of different stator winding connections on torque-speed and efficiency characteristics are investigated. Then, the complete electric machine drive system including the winding changeover mechanism is simulated to present its dynamic performance. Finally, experimental results are provided to show the transient and steady-state operation of a five-phase PMSM with star, pentagon, and pentacle winding connections.
Novel noncontact thickness metrology for backend manufacturing of wide bandgap light emitting devices (2005)

Low coherence optical interferometry (D. Huang et al., Science 254, 1178 (1991) [1]) has been proven to be an effective tool for characterisation of thin and ultra-thin semiconductor Si wafers (W. J. Walecki et al., 3rd International Workshop on Thin Semiconductor Devices – Manufacturing and Applications, Munich, Germany, November 25, 2002; Q. Wang et al., phys. stat. sol. (a) 155, 289 (1996) [2]). Purpose of this paper is to present an extension of this method to characterisation of ultra-thin compound wide bandgap wafers mounted on insulating carriers. We present alternative technique, which does not suffer from above discussed limitations, and can be easily integrated in the production tools, and provide accurate measurement of samples while they are being processed. (© 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
Sulfobenzoic Acid Esters. III. The Correct Structures of the Aryl Esters (1962)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSulfobenzoic Acid Esters. III. The Correct Structures of the Aryl EstersBernard Loev and Minerva KormendyCite this: J. Org. Chem. 1962, 27, 7, 2448–2452Publication Date (Print):July 1, 1962Publication History Published online1 May 2002Published inissue 1 July 1962https://pubs.acs.org/doi/10.1021/jo01054a038https://doi.org/10.1021/jo01054a038research-articleACS PublicationsRequest reuse permissionsArticle Views166Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Single stranded adeno-associated virus achieves efficient gene transfer to anterior segment in the mouse eye (2017)

Adeno-associated viruses (AAVs) are used extensively as a gene delivery vehicle for retinal gene therapy, yet its ability to target the anterior segment of the eye, critical to unlocking therapeutic opportunities, is less characterized. Previously, self-complimentary (sc) AAV was shown to be necessary for transduction of the cornea and trabecular meshwork (TM), limiting the size of the gene transfer cassette, likely due to a block in second strand synthesis thought to be required for functional transduction. Here, we evaluated several AAV capsids in a single stranded (ss) genome conformation for their ability to overcome the need for scAAV for targeting corneal endothelium and TM. AAV2, 8, and a recently synthetically developed AAV called Anc80L65 were evaluated in vitro and in vivo by intracameral injection in mice. Results show that although scAAV2 demonstrated superior infectivity in vitro including Human Trabecular meshwork (HTM) immortalized cell lines; Anc80L65 transduced following a single intracameral injection efficiently all components of the mouse anterior segment, including the TM, corneal stroma, and endothelial cells. These results suggest that Anc80L65 is able to overcome the requirement for scAAV genomes to enable TM and corneal targeting, expanding the potential experimental and therapeutic use of AAV gene transfer in the anterior segment of the eye.
Improved MLLR speaker adaptation using confidence measures for conversational speech recognition (2000)

Automatic recognition of conversational speech tends to have higher word error rates (WER) than read speech. Improvements gained from unsupervised speaker adaptation methods like Maximum Likelihood Linear Regression (MLLR) [1] are reduced because of their sensitivity to recognition errors in the first pass. We show that a more detailed modeling of adaptation classes and the use of confidence measures improve the adaptation performance. We present experimental results on the VERBMOBIL task, a German conversational speech corpus.
Accurate Prediction of Peptide Binding Sites on Protein Surfaces (2009)

Many important protein-protein interactions are mediated by the binding of a short peptide stretch in one protein to a large globular segment in another. Recent efforts have provided hundreds of examples of new peptides binding to proteins for which a three-dimensional structure is available (either known experimentally or readily modeled) but where no structure of the protein-peptide complex is known. To address this gap, we present an approach that can accurately predict peptide binding sites on protein surfaces. For peptides known to bind a particular protein, the method predicts binding sites with great accuracy, and the specificity of the approach means that it can also be used to predict whether or not a putative or predicted peptide partner will bind. We used known protein-peptide complexes to derive preferences, in the form of spatial position specific scoring matrices, which describe the binding-site environment in globular proteins for each type of amino acid in bound peptides. We then scan the surface of a putative binding protein for sites for each of the amino acids present in a peptide partner and search for combinations of high-scoring amino acid sites that satisfy constraints deduced from the peptide sequence. The method performed well in a benchmark and largely agreed with experimental data mapping binding sites for several recently discovered interactions mediated by peptides, including RG-rich proteins with SMN domains, Epstein-Barr virus LMP1 with TRADD domains, DBC1 with Sir2, and the Ago hook with Argonaute PIWI domain. The method, and associated statistics, is an excellent tool for predicting and studying binding sites for newly discovered peptides mediating critical events in biology.
Advances in Solid-State Transformations of Coordination Bonds: From the Ball Mill to the Aging Chamber (2017)

Controlling the formation of coordination bonds is pivotal to the development of a plethora of functional metal-organic materials, ranging from coordination polymers, metal-organic frameworks (MOFs) to metallodrugs. The interest in and commercialization of such materials has created a need for more efficient, environmentally-friendly routes for making coordination bonds. Solid-state coordination chemistry is a versatile greener alternative to conventional synthesis, offering quantitative yields, enhanced stoichiometric and topological selectivity, access to a wider range of precursors, as well as to molecules and materials not readily accessible in solution or solvothermally. With a focus on mechanochemical, thermochemical and "accelerated aging" approaches to coordination polymers, including pharmaceutically-relevant materials and microporous MOFs, this review highlights the recent advances in solid-state coordination chemistry and techniques for understanding the underlying reaction mechanisms.
Extinction of fear-potentiated startle: blockade by infusion of an NMDA antagonist into the amygdala (1992)

Data derived from in vitro preparations indicate that NMDA receptors play a critical role in synaptic plasticity in the CNS. More recently, in vivo pharmacological manipulations have suggested that an NMDA-dependent process may be involved in specific forms of behavioral plasticity. All of the work thus far has focused on the possible role of NMDA receptors in the acquisition of responses. However, there are many examples in the behavioral literature of learning-induced changes that involve the reduction or elimination of a previously acquired response. Experimental extinction is a primary example of the elimination of a learned response. Experimental extinction is well described in the behavioral literature, but has not received the same attention in the neurobiological literature. As a result, the neural mechanisms that underlie this important form of learning are not at all understood. In the present experiments, the fear-potentiated startle paradigm was employed to begin to investigate neural mechanisms of extinction. The results show that infusion of the NMDA antagonist D,L-2-amino-5-phosphonovaleric acid (AP5) into the amygdala, a limbic structure known to be important for fear conditioning, dose-dependently blocked extinction of conditioned fear. Control experiments showed that the blockade of extinction was neither the result of the permanent disruption of amygdaloid function nor the result of decreased sensitivity of the animals to the conditioned stimulus. Infusion of AP5 into the interpositus nucleus of the cerebellum, a control site, did not block extinction. Finally, intra-amygdala infusion of a selected dose of the non-NMDA antagonist 6-cyano-7-nitroquinoxaline-2,3-dione did not block extinction of conditioned fear. These results, together with a previous report from our laboratory (Miserendino et al., 1990), demonstrate the importance of the amygdala in the elaboration of conditioned fear and suggest that an NMDA-dependent process might underlie the extinction of conditioned fear.
The Synthesis and Evaluation of Some Coumarin Derivatives as Fluorescent Indicators of Nitroreductase Activity. (2006)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.
Characterization of the local temperature in space and time around a developing Drosophila embryo in a microfluidic device (2006)

This paper characterizes a microfluidic platform that differentially controls the temperature of each half of a living Drosophila melanogaster fruitfly embryo in space and time (E. M. Lucchetta, J. H. Lee, L. A. Fu, N. H. Patel and R. F. Ismagilov, Nature, 2005, 434, 1134-1138). This platform relies on laminar flow of two streams of liquid with different temperature, and on rapid prototyping in polydimethylsiloxane (PDMS). Here, we characterized fluid flow and heat transport in this platform both experimentally and by numerical simulation, and estimated the temperature distribution around and within the embryo by numerical simulation, to identify the conditions for creating a sharper temperature difference (temperature step) over the embryo. Embryos were removed from the device and immunostained histochemically for detection of Paired protein. Biochemical processes are sensitive to small differences in environmental temperature. The microfluidic platform characterized here could prove useful in understanding dynamics of biochemical networks as they respond to changes in temperature.
A phase I clinical trial of novobiocin, a modulator of alkylating agent cytotoxicity. (1991)

Antineoplastic drug resistance is a major obstacle to improved treatment of most adult cancers in humans. Novobiocin, an antibacterial agent which inhibits the eukaryotic topoisomerase II enzyme, increases the cytotoxicity of several alkylating agents in vitro by the formation of lethal DNA-DNA interstrand cross-links, perhaps by decreasing the repair of drug monoadducts. In murine tumors treated in vivo novobiocin markedly potentiates alkylating agent cytotoxicity without concomitant increases in host toxicity. With this background, a Phase I trial of novobiocin and cyclophosphamide was performed in refractory cancer patients. Novobiocin was given p.o. for 96 h; 750 mg/m2 of i.v. cyclophosphamide was administered at 48 h. Thirty-four patients received 65 courses. The dose-limiting toxicity of novobiocin in this trial was vomiting. The maximum tolerated dose was 6 g/day. Six of 34 patients had Grade III or IV mylosuppression but no dose escalation effect was noted. Three patients developed allergic reactions which resolved completely. No other significant toxicity occurred. While no dose-dependent effect on serum novobiocin levels occurred, 18 of 19 patients treated at greater than or equal to 4 g daily had serum levels greater than or equal to 100 micrograms/ml at steady state, a level which corresponds to levels used in vitro and seen in vivo where the murine novobiocin half-life of 82 min is far less than that seen in humans (6.0 h). Two of 30 evaluable patients had partial responses. Four other patients had stable disease. Four of six had prior disease progression on cyclophosphamide combination therapy. Novobiocin is well tolerated in patients receiving cyclophosphamide and blood levels are in the drug-potentiating range. Phase II trials in cyclophosphamide refractory patients are anticipated.
Surface-Charge Lithography for Direct PDMS Micro-Patterning (2008)

Direct patterning of PDMS films is achieved by modulating the wettability of polar dielectric substrates. Periodic array structures of microbumps can be madeup by functionalizing periodically poled lithium niobate crystals. The modulation of surface wettability is obtained through the spatial distribution of the surface electric charges generated by the pyroelectric effect under electrode-less configuration. An appropriate thermal treatment of the substrates assures both the wettability patterning and the fast cross-linking of the PDMS film.