gebhart/patrepeval huggingface dataset:
Identification of a Novel Antiapoptotic Human Protein Kinase C δ Isoform, PKCδVIII in NT2 Cells (2007)

Protein kinase C (PKC) delta plays an important role in cellular proliferation and apoptosis where it is involved in the caspase-3 mediated apoptotic pathway. Cleavage of PKCdeltaI by caspase-3 releases a catalytically active C-terminal fragment that is sufficient to induce apoptosis. In this paper, we identified a novel human PKCdelta isozyme, PKCdeltaVIII (Genbank accession number DQ516383) in human teratocarcinoma (NT2) cells that differentiate into hNT neurons upon retinoic acid (RA) treatment. Expression of PKCdeltaVIII was confirmed by real-time RT-PCR analysis, and we observed that after an initial peak at 24 h following RA treatment, its expression gradually declined with prolonged RA treatment. PKCdeltaVIII is generated via the utilization of an alternative 5' splice site, and this results in an insertion of 31 amino acids in the caspase-3 recognition sequence DMQD. The function of PKCdeltaVIII was examined by subcloning it into an expression vector and raising an antibody specific to PKCdeltaVIII. Using in vivo and in vitro assays, we demonstrated that PKCdeltaVIII is resistant to caspase-3 cleavage. Next, we sought to determine the role of PKCdeltaVIII in apoptosis in NT2 cells. Overexpression of PKCdeltaVIII and knockdown using PKCdeltaVIII siRNA suggest an antiapoptotic function for the PKCdeltaVIII isozyme. We demonstrate that antisense oligonucleotides (ASO) directed toward the 5' splice site I promote the expression of the PKCdeltaVIII isozyme. Our results indicated that ASO mediated PKCdeltaVIII expression rescued NT2 cells from etoposide-induced apoptosis. We conclude that the novel human PKCdeltaVIII splice variant functions as an antiapoptotic protein in NT2 cells.
Mechanistic investigation of the copper-catalyzed reactions of diphenyliodonium salts (1983)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMechanistic investigation of the copper-catalyzed reactions of diphenyliodonium saltsThomas P. LockhartCite this: J. Am. Chem. Soc. 1983, 105, 7, 1940–1946Publication Date (Print):April 1, 1983Publication History Published online1 May 2002Published inissue 1 April 1983https://pubs.acs.org/doi/10.1021/ja00345a045https://doi.org/10.1021/ja00345a045research-articleACS PublicationsRequest reuse permissionsArticle Views1696Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Percutaneous absorption of hydroquinone in humans: Effect of 1‐dodecylazacycloheptan‐2‐one (azone) and the 2‐ethylhexyl ester of 4‐(dimethylamino)benzoic acid (escalol 507) (1988)

Hydroquinone was found to penetrate readily human forehead skin in vivo following a single topical exposure, in an alcoholic vehicle, of 24 h duration. Percutaneous absorption was estimated using radiotracer methodology and 14C-labeled hydroquinone. The effects of a penetration enhancer, 1-dodecylazacycloheptan-2-one, and a sunscreen, the 2-ethylhexyl ester of 4-(dimethylamino)benzoic acid, on the percutaneous absorption of hydroquinone were investigated. In vivo penetration of hydroquinone was significantly decreased (a less than 0.05) by the addition of the 2-ethylhexyl ester of 4-(dimethylamino)benzoic acid (3% w/w) to the vehicle. The penetration enhancer, 1-dodecylazacycloheptan-2-one (0.5% w/w), did not significantly increase (a greater than 0.05) the absorption of hydroquinone. From all hydroquinone preparations, percutaneous absorption was rapid and peak elimination occurred within the first 12 h following application. Elimination was complete within 5 d.
[Alcohol and neoplasms of the brain]. (1989)

The incidence of cerebral tumors among mentally ill patients was 5.3 per 10,000. In a verified series of brain diseases (131 observations) alcoholism was revealed in 40 cases, which either modified the clinical picture of the disease or provoked a number of mental disorders, such as focal epileptic seizures, Korsakoff, or "frontal" syndromes. Alcohol abuse was regarded as a sign of cerebral tumor in a some patients. Reducing the central nervous system compensation abilities, alcohol promoted the malignant development of species of cerebral tumors causing skyrocketing rapid course.
Aromatization of dihydrothiophenes. Thiophenesaccharin: a sweet surprise (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAromatization of dihydrothiophenes. Thiophenesaccharin: a sweet surprisePhillip A. Rossy, Werner Hoffmann, and Norbert MuellerCite this: J. Org. Chem. 1980, 45, 4, 617–620Publication Date (Print):February 1, 1980Publication History Published online1 May 2002Published inissue 1 February 1980https://pubs.acs.org/doi/10.1021/jo01292a012https://doi.org/10.1021/jo01292a012research-articleACS PublicationsRequest reuse permissionsArticle Views807Altmetric-Citations60LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Optimal posture of a human operator and CAD in robotics (2002)

In complex mechanical systems, the important features to be considered in the earliest phases of assembly design are: the operators accessibility for assembly operations and maintenance checking. We present in this paper a new approach for solving this problem by considering the human operator as a manipulator, taking into account the geometrical and biomechanical constraints. They are integrated by penalizing a global criterion. The solution of the associated optimization problem is obtained with simulated annealing algorithm.
Experimental entangled photon pair generation using crystals with parallel optical axes (2018)

We present an optical design where polarization-entangled photon pairs are generated within two β-Barium Borate crystals whose optical axes are parallel. This design increases the spatial mode overlap of the emitted photon pairs enhancing single mode collection without the need for additional spatial walk-off compensators. The observed photon pair rate is at least 65 000 pairs/s/mW with a quantum state fidelity of 99.53 ± 0.22% when pumped with an elliptical spatial profile.
Off-resonant manipulation of spins in diamond via precessing magnetization of a proximal ferromagnet (2014)

Photoluminescence measurements show that the spin state of an $N\phantom{\rule{0}{0ex}}V$ center can be modified nonresonantly (i.e. at frequencies far from the $N\phantom{\rule{0}{0ex}}V$ Larmor frequencies) via coupling to the dynamic magnetization of a ferromagnet---an unexpected result that presents a new approach to transferring ferromagnetic spin information into a paramagnet.
Autofocusing and Polar Body Detection in Automated Cell Manipulation (2016)

Autofocusing and feature detection are two essential processes for performing automated biological cell manipulation tasks. In this paper, we have introduced a technique capable of focusing on a holding pipette and a mammalian cell under a bright-field microscope automatically, and a technique that can detect and track the presence and orientation of the polar body of an oocyte that is rotated at the tip of a micropipette. Both algorithms were evaluated by using mouse oocytes. Experimental results show that both algorithms achieve very high success rates: 100% and 96%. As robust and accurate image processing methods, they can be widely applied to perform various automated biological cell manipulations.
TAG-72 (CA 72-4 assay) as a complementary serum tumor antigen to carcinoembryonic antigen in monitoring patients with colorectal cancer (1993)

The findings support previous observations of complementary expression of TAG-72 and CEA and indicate that a significant advantage could be gained in the detection of primary and, perhaps, recurrent colorectal carcinoma by incorporating the measurement of serum TAG-72 with that of CEA.
Secure identification documents via pattern recognition and public-key cryptography (1998)

We present an approach to authenticating photo-ID documents that relies on pattern recognition and public-key cryptography and has security advantages over physical mechanisms that currently safeguard cards, such as optical laminates and holograms. The pattern-recognition component of this approach is based on a photo signature that is a concise representation of the photo image on the document. This photo signature is stored in a database for remote authentication or in encrypted form on the card for stand-alone authentication. Verification of the ID document entails scanning both the photo image and a machine-readable form of the text information, determining the photo signature, and comparing this information against the reference. In this paper, we describe the method and present results of testing a large database of images for photo-signature match in the presence of noise.
Enzymatic Hydrolysis of Sphingolipids. VI. Hydrolysis of Ceramide Glycosides by Calf Brain β-N-Acetylhexosaminidase<sup>*</sup> (1967)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzymatic Hydrolysis of Sphingolipids. VI. Hydrolysis of Ceramide Glycosides by Calf Brain β-N-Acetylhexosaminidase*Yaacov Zvi Frohwein and Shimon GattCite this: Biochemistry 1967, 6, 9, 2783–2787Publication Date (Print):September 1, 1967Publication History Published online1 May 2002Published inissue 1 September 1967https://pubs.acs.org/doi/10.1021/bi00861a019https://doi.org/10.1021/bi00861a019research-articleACS PublicationsRequest reuse permissionsArticle Views49Altmetric-Citations47LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Science versus Pseudoscience, Truth versus Lies (2006)

Two scientific presentations are outlined, one which gave impressive insights, and another that provided alarming conclusions. The second presentation indicated that the future of energy is hydrogen, derived from nuclear fusion. However that approach is completely wrong since globalisation of trade and profit opportunities requires that responsibilities for a sustainable future and for environmental conservation be shared globally. In this light surely it is reasonable to expect that the nations, which are the highest per capita consumers of fossil fuels, ought to bear the greatest responsibilities and set the best examples.
TAUX D'UREE DU LAIT, ALLANTOINE URINAIRE, TEMOINS DE LA NUTRITION AZOTEE CHEZ LA CHEVRE EN LACTATION (1984)

not available
Extracellular nucleases of Pseudomonas BAL 31. III. Use of the double-strand deoxyriboexonuclease activity as the basis of a convenient method for the mapping of fragments of DNA produced by cleavage with restriction enzymes (1978)

We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished without the introduction of detectable scissions away from the ends of the duplexes. When this nuclease is used to produce a series of progressively shortened samples from a linear duplex DNA, subsequent digestion of these samples with a site-specific restriction endonuclease and analysis of the resulting fragments by gel electrophoresis permits the rapid establishment of the order of the restriction enzyme fragments through the entire genome. This is accomplished by noting from the electropherograms the order in which the various restriction enzyme fragments become noticeably shortened or disappear. Using this method, the five cleavage sites for the endonuclease Hpa I and the single cleavage sites for the nucleases Hpa II and Pst I have been mapped in PM2 bacteriophage DNA. In a more stringent test of the method, 18 of the 24 fragments produced by cleavage of coliphage lambdab2b5c DNA with the Pst I nuclease have been mapped, and five of the six remaining fragments have been assigned to small regions of the genome.
The effects of genistein, a tyrosine kinase inhibitor on acute and chronic inflammation in diabetic mice (2006)

The effects of tyrosine kinases on acute and chronic inflammation during diabetes are not fully determined. Therefore, the present study focuses on the effects of genistein, a tyrosine kinase inhibitor, on acute and chronic inflammation in diabetic mice. The mice either received normal saline (control, 0.1 ml, i.p., n=144) or streptozotocin (diabetic, STZ, 200 mg kg-1, i.p., n=144). A week after injection of saline or STZ acute and chronic inflammation was induced by injecting carrageenan and implanting 2 cotton pellets. Before injection carrageenan or 5 day after implantation, 9 mice from each group (control or diabetic) received genistein (10 mg kg-1, i.p.), indomethacin (2 mg kg-1, i.p.) or L-NAME (0.1 mg kg-1, i.p.). Paw edema and the weight of cotton pellets were significantly higher in diabetic mice. Pretreatment with either indomethacin or L-NAME significantly reduced the acute and chronic inflammation in the diabetic group. Genistein reduced chronic inflammation significantly. These results suggest that activation of tyrosine kinases as well as prostaglandins and nitric oxide pathways are involved in the increased chronic inflammatory responses observed in the diabetic animals.
Zum Problem der <i>pn</i>‐Übergänge zwischen organischen und anorganischen Photoleitern (1965)

Abstract In Fortsetzung der Untersuchungen über organische und organisch/anorganische pn ‐Übergänge wird das photoelektrische Verhalten von Silberhalogenid/Farbstoff‐Systemen geprüft. Die Eigenschaften des photovoltaischen Effekts dieser Systeme werden beschrieben sowie einige Regeln diskutiert, die die Richtung der Photospannungen und Photoströme in verschiedenen Kombinationen aus n ‐ oder p ‐Halbleitern und p ‐ oder n ‐leitenden Farbstoffen angeben.
Identification of a GBR12935 homolog, LR1111, which is over 4,000‐fold selective for the dopamine transporter, relative to serotonin and norepinephrine transporters (1993)

The di-substituted piperazines, GBR12909 (1-[2-[bis(4-fluorophenyl)-methoxy]ethyl]-4-[3- phenylpropyl]piperazine) and GBR12935 (1-[2-(diphenyl-methoxy)-ethyl]-4-(3-phenylpropyl)piperazine), are potent and selective (20-to 100-fold) inhibitors of [3H]dopamine reuptake, relative to [3H]5-HT and [3H]norepinephrine uptake. The GBR12935 analog, 1-(2-(diphenylmethoxy)ethyl)-4-(3-phenylpropyl)homopiperazine (LR1111), was synthesized as part of a systematic structure-activity study of analogs of GBR12935 and GBR12909. LR1111 differs from GBR12935 by the addition of a methylene group into the piperazine ring to yield a compound with a seven-member homopiperazine ring. The IC50 values for LR1111 at the dopamine, norepinephrine, and serotonin transporters were 7.2 nM, 34,072 nM, and greater than 20,000 nM, respectively, whereas the IC50 values of GBR12935 were 3.7 nM, 289 nM, and 1261 nM for these same transporters. This demonstrates that the addition of a single methylene group in the piperazine ring results in a compound with similar affinity but significantly higher selectivity for the dopamine transporter. LR1111 increased motoric activity in rats after intravenous administration. These indicate that LR1111 is a potent and highly selective inhibitor of the dopamine transporter.
Une approche à la SQL du traitement de données intensif dans Gaspard (1999)

(In French)
A facile solvent‐free synthesis of 1‐alkyl/aralkyl‐2‐(1‐arylsulfonyl alkyl) benzimidazoles using “TBAB” as surface catalyst (2010)

Abstract magnified image Reaction 2‐(α‐chloroalkyl)benzimidazoles 1 with aryl sulphinate sodium salt 2 under solvent‐free conditions in the presence of tetrabutylammonium bromide as surface catalyst, by simple physical grinding using mortar and pestle, gives 1 H ‐2‐(α‐arylsulfonylalkyl)benzimidazoles 3 . The latter on treatment with alkylating agents under solvent‐free conditions results in 1‐alkyl/aralkyl‐2‐(α‐arylsulfonylalkyl)benzimidazoles 4 . Alternatively, 4 can also be prepared directly from 1‐alkyl/aralkyl‐2‐(α‐chloroalkyl)benzimidazoles 5 by reaction with 2 , which in turn could be prepared by reaction of 1 with alkylating agents under solvent‐free conditions and all these reactions are free from organic solvents including experimental procedures. J. Heterocyclic Chem., (2010).
The state of prostate MRI in 2013. (2013)

Compared with earlier prostate MRI techniques that suffered from relatively poor sensitivity and specificity for detecting prostate cancer because of their reliance predominantly on morphology, multiparametric prostate MRI (mpMRI) in 2013 provides a wealth of functional information that has made possible vastly improved detection and characterization of prostate cancer. Our aims in this article are to describe the various imaging sequences that comprise the mpMRI exam, as well as to review current literature on the strengths/weaknesses of these sequences; to delineate strategies for standardizing interpretation and reporting of MRI results; and finally, to expound on the role that prostate MRI currently does and potentially can play in clinical practice.
Morphology and fertilizability of frozen human oocytes (1987)

Human oocytes were frozen and thawed by four methods previously used for cryopreservation of human embryos. Most of these oocytes were inseminated after thawing to assess their capacity to fertilize and form pronuclear ova. Their morphology was assessed by phase-contrast microscopy used in routine IVF. Twenty-three oocytes were examined by electron microscopy to critically evaluate the effects of cooling and cryopreservation and to confirm fertilization. Morphological survival was observed in more than 60% of the oocytes examined after freeze-thawing. The main features of cryoinjury were cracks in the zona pellucida, disruption of the plasma membrane and extensive disorganization of the ooplasm. Subtle changes in the cytosol of cumulus cells was also observed. Cooling to 0 degrees C or -6 degrees C had little effect on cytoplasmic structure. Spindles were damaged in two frozen oocytes. Cumulus cell activity, sperm binding to the zona, sperm penetration of the zona seem to be largely unaffected by freeze-thawing. Fertilization was observed in eight oocytes after postthaw insemination and three embryos (8-cell to morula stages) were developed from pronuclear ova on further culture. Both monospermic and polyspermic fertilization were confirmed by electron microscopy and micronuclei were detected in three pronuclear ova. The genetic implications of these nuclear aberrations are discussed. These preliminary studies indicate that oocyte freezing needs to be integrated cautiously with clinical IVF by further assessment of embryos developed from frozen oocytes.
Investigation of the Crystallization Process of SAPO-35 and Si Distribution in the Crystals (2013)

The hydrothermal crystallization of SAPO-35 molecular sieve is examined in the present study. Many characterization methods including XRD, SEM, XRF, XPS, EDS, and various solid-state NMR techniques are applied to monitor the formation process of SAPO-35 and the development of the local environments of Si, P, and Al atoms as a function of crystallization time. The results show that an intermediate phase with ordered structure and AlPO compositions first forms. Subsequently, SAPO-35 crystallizes at the expense of the intermediate phase. Si takes part in the formation of SAPO-35 since the initial crystallization. The Si concentration in SAPO-35 shows an increasing trend with time, which leads to the change of both Si and Al coordination environments. More information is obtained from two-dimensional 27Al MQ MAS and 27Al → 31P MQ-MAS HETCOR NMR. Moreover, XPS analysis reveals the enrichment of Si on the crystal surface. On the basis of the characterization results, it is supposed that the distribution of Si in the SAPO-35 crystals is not uniform, showing a gradual increase from the core to the external surface.