gebhart/patrepeval huggingface dataset:
Duocalins: Engineered Ligand-Binding Proteins with Dual Specificity Derived from the Lipocalin Fold (2001)

Anticalins comprise a novel class of receptor proteins with predetermined ligand specificities which were engineered using the lipocalin fold. Attractive features of these artificial ligand-binding proteins include their small size and monomeric nature, being composed of a single polypeptide chain. Here we report the construction of a functional fusion protein from two independent anticalins, a so-called duocalin. The gene for the fusion protein was assembled from nucleotide sequences encoding an anticalin with fluorescein specificity on the one hand and an anticalin with digoxigenin specificity on the other. Both engineered lipocalins were previously selected from a random library prepared on the basis of the bilin-binding protein, a natural lipocalin abundant in insects. The corresponding fusion protein was expressed in a secretable form in E. coli cells and isolated from the periplasmic fraction using the Strep-tag method. The major fraction of the purified protein appeared to possess the proper pattern of altogether four disulphide bonds. The ligand-binding behaviour of the fusion protein was investigated both by solid phase ELISA and in fluorescence titration experiments. Our results demonstrate that the novel fusion protein has retained both ligand specificities. Up to now, dimerized ligand-binding proteins were mostly derived from recombinant antibody fragments. Compared with those constructs the duocalins, either with bispecific or with bivalent target recognition properties, should provide useful reagents for various purposes in biotechnology.
Detecting global motion patterns in complex videos (2008)

Learning dominant motion patterns or activities from a video is an important surveillance problem, especially in crowded environments like markets, subways etc., where tracking of individual objects is hard if not impossible. In this paper, we propose an algorithm that uses instantaneous motion field of the video instead of long-term motion tracks for learning the motion patterns. The motion field is a collection of independent flow vectors detected in each frame of the video where each flow is vector is associated with a spatial location. A motion pattern is then defined as a group of flow vectors that are part of the same physical process or motion pattern. Algorithmically, this is accomplished by first detecting the representative modes (sinks) of the motion patterns, followed by construction of super tracks, which are the collective representation of the discovered motion patterns. We also use the super tracks for event-based video matching. The efficacy of the approach is demonstrated on challenging real-world sequences.
EMI Failure Analysis Techniques: II. Joint Time-Frequency Analysis (2010)

Time domain and frequency domain are two extremes of a large set of signal analysis techniques. Time domain gives the best time resolution, but no direct frequency information. The best frequency resolution is obtained in the frequency domain where,
Development of an opto-chemical carbon dioxide sensor for aquaculture and oceanography applications (2011)

In this paper an optical carbon dioxide (CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ) lifetime-based sensor designed for the continuous monitoring of low CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> levels in aqueous solution in fields of fish farming and in oceanography is described. The presented optical measurement unit consists of a CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> sensitive layer mounted onto the end of sensing probe and an optoelectronic interrogation unit in order to convert the measurement signal into detectable signal. The measurement technique is the phase modulation fluorometry using Resonance Energy Transfer (RET) as sensing scheme. RET measurements were made by incorporation of ruthenium-(II)-tis-4,7-diphenyl-1,10-phennathroline (Rudpp) <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> - together with pH indicator ion-paired with tetraoctylammonium (TOA-OH) as the cunterion in a highly gas-permeable matrix. As a protective coating a high gas permeable Teflon with added graphite particles was used to eliminate interferences by ionic species. The performance of the whole sensor system is presented with respect to spectral characteristics, dynamic range (0 - 60 mg/l of dissolved CO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> ), required sensor system specifications, temperature behavior and long term stability. First field tests performed in real fish farming conditions are presented as well.
Structure-Activity–Dependent Regulation of Cell Communication by Perfluorinated Fatty Acids using <i>in Vivo</i> and <i>in Vitro</i> Model Systems (2008)

The in vitro analysis of GJIC, an epigenetic marker of tumor promoters, can also predict the in vivo activity of PFOA, which dysregulated GJIC via ERK and PC-PLC.
Oxygen Electroreduction Catalyzed by Gold Nanoclusters: Strong Core Size Effects (2009)

Bigger isn't better: Gold nanoclusters exhibit enhanced electrocatalytic activity in oxygen reduction when the core size decreased from Au(140) to Au(11), as reflected by the onset potential E(onset) and peak current density J(K) (see picture). The increasing fraction of surface atoms with low coordination numbers and the shift of the d-band center to the Fermi level facilitate oxygen adsorption.
Polycythemia Vera: The Natural History of 1213 Patients Followed for 20 Years (1995)

Cytoreduction favorably affects the incidence of thrombotic events, but aggressive treatment seems to be associated with increased risk for neoplasm. These results provide a basis for reevaluating the therapeutic strategy in patients with polycythemia vera and for estimating the size of clinical trials aimed at testing new therapeutic approaches.
Murine Stromal Cells Counteract the Loss of Long-Term Culture-Initiating Cell Potential Induced by Cytokines in CD34+CD38low/neg Human Bone Marrow Cells (1999)

Evidence has been provided recently that shows that high concentrations of cytokines can fulfill functions previously attributed to stromal cells, such as promote the survival of, and led to a net increase in human primitive progenitors initiating long-term cultures in vitro (LTC-IC) or engrafting NOD-SCID (nonobese diabetic severe-combined immunodeficient) recipients in vivo. These data prompted us to re-evaluate whether stromal cells will further alter the properties of primitive progenitor cells exposed to cytokines. Single CD34+CD38low and CD38neg cells were incubated 10 days in serum-containing or serum-free medium in the presence or in the absence of murine marrow-derived stromal cells (MS-5). Recombinant human cytokines stem cell factor (SCF), pegylated-megakaryocyte growth and differentiation factor (PEG–MGDF), FLT3-L, Interleukin (IL)-3, IL-6, and granulocyte-macrophage colony-stimulating factor (GM–CSF) were systematically added at various concentrations (10 to 300 ng/mL). Cell proliferation and LTC-IC potential were evaluated in each clone after 10 days. A striking and consistent observation was the retention of a high LTC-IC potential in clones exposed to cytokines in the presence of stromal feeders, whereas clones exposed to cytokines alone in the absence of stromal feeders rapidly lost their LTC-IC potential as they proliferated. This was reflected both by the higher proportion of wells containing LTC-IC and by the high numbers of CFC produced after 5 weeks in clones grown with MS-5 during the first 10 days. We further showed by analyzing multiple replicates of a single clone at day 10 that MS-5 cells promoted a net increase in the LTC-IC compartment through self-renewal divisions. Interestingly, these primitive LTC-IC were equally distributed among small and large clones, as counted at day 10, indicating that active proliferation and loss of LTC-IC potential could be dissociated. These observations show that, in primitive cells, stromal cells counteract differentiation events triggered by cytokines and promoted self-renewal divisions. Furthermore, the almost identical distribution of the size of the clones with or without MS-5 suggests that proliferation and function of human primitive cells may be independently regulated by external signals, and that the former is primarily under the control of cytokines.
An amino-terminal fragment of LCRF, LCRF-(1-35), has the same activity as the natural peptide (1997)

A cholecystokinin (CCK)-releasing peptide, luminal CCK-releasing factor (LCRF), has been purified from rat jejunal secretion. Amino acid analysis and mass spectral analysis showed that the purified peptide is composed of 70-75 amino acid residues and has a mass of 8,136 Da. Microsequence analysis of LCRF yielded an amino acid sequence for the amino-terminal 41 residues. To determine the biologically active region of the molecule, a peptide was synthesized consisting of the amino-terminal 35 amino acids of LCRF. In this study, intraduodenal infusion of LCRF-(1-35) significantly stimulated pancreatic secretion in conscious rats. The dose-response curves to LCRF-(1-35) and to monitor peptide were similar and biphasic, with higher doses producing submaximal pancreatic secretory responses. The CCK-A receptor antagonist MK-329 abolished the pancreatic secretory response to intraduodenally infused LCRF-(1-35). These results demonstrate that LCRF biological activity is contained within the amino-terminal 35-amino acid portion of LCRF, and this fragment may be useful for investigating the role of LCRF in gastrointestinal function.
A Delphi Study of RFID Applicable Business Processes and Value Chain Activities in Retail (2011)

For this research we use Delphi technique to identify the key business processes and value chain activities that are improved by RFID. Our Delphi study involves 74 experts from different domains such as consulting, retail, academia, and third party service providers. We also explored whether there is any difference in expert perceptions about RFID applicable business processes and value chain activities across different business associations.
Inhibitory effects of 2-<i>O</i>-octadecylascorbic acid and other vitamin C and E derivatives on the induction of enzyme-altered putative preneoplastic lesions in the livers of rats fed a choline-deficient, L-amino acid-defined diet (1994)

Effects of a lipophilic derivative of vitamin C, 2-O-octadecylascorbic acid (CV-3611), as well as its parent L-ascorbic acid (AscA), DL-alpha-tocopherol (alpha-T) and its hydrophilic derivative, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), on the number and size of gamma-glutamyltransferase (GGT)-positive putative preneoplastic lesions were examined and compared with their influences on 8-hydroxyguanine formation in DNA and 2-thiobarbituric acid-reacting substance generation in the livers of rats fed a choline-deficient, L-amino acid-defined (CDAA) diet for 12 weeks. A total of 90 male Fischer 344 rats, 6 weeks old, were divided into 18 groups each consisting of five rats. Group 1 received the CDAA diet alone; Groups 2, 3 and 4 received the CDAA diet containing respectively 0.01, 0.05 and 0.10% CV-3611; Groups 5-7, 8-10 and 11-13 similarly received the CDAA diet containing AscA, alpha-T and Trolox, respectively, at these same low, middle and high concentrations; Group 14 received a choline-supplemented, L-amino acid-defined (CSAA) diet alone; Groups 15-18 were given the CSAA diet containing CV-3611, AscA, alpha-T and Trolox, respectively, all at the 0.10% level. While all four vitamin derivatives exerted inhibitory effects on all four parameters, in each case dose-dependently, CV-3611 demonstrated the most pronounced effects. The present results indicated that lipophilic vitamin C derivatives may be particularly effective chemopreventive agents against CDAA diet-associated, oxidative stress-related hepatocarcinogenesis via its superior antioxidative properties.
An Effective Missing-Data Estimation Approach for Small-Size Image Sequences (2015)

Missing data is a frequently encountered problem for structure-from-motion (SFM) where the 3D structure of an object is estimated based on 2D images. In this paper, an effective approach is proposed to deal with the missing-data estimation problem for small-size image sequences. In the proposed method, a set of sub-sequences is first extracted. Each sub-sequence is composed of the frame to be estimated and a part of the original sequence. In order to obtain diversified estimations, multiple weaker estimators are constructed by means of the column-space-fitting (CSF) algorithm. The various sub-sequences are in turn used as the inputs to the algorithm. As the non-missing entries are known, the estimation errors of these entries are computed so as to select weaker estimators with better estimation performances. Furthermore, a linear programming based weighting model is established to compute the weights for the selected weaker estimators. After the weighting coefficients are obtained, a linear weighting estimation which is used as the final estimation of the missing entries is computed based on the outputs of the weaker estimators. By applying the strategies of weaker-estimator selection and the linear programming weighting model, the proposed missing entry estimation method is more accurate and robust than the existing algorithms. Experimental results on several widely used image sequences demonstrate the effectiveness and feasibility of the proposed algorithm.
Renal progenitors derived from human iPSCs engraft and restore function in a mouse model of acute kidney injury (2015)

Acute kidney injury (AKI) is one of the most relevant health issues, leading to millions of deaths. The magnitude of the phenomenon remarks the urgent need for innovative and effective therapeutic approaches. Cell-based therapy with renal progenitor cells (RPCs) has been proposed as a possible strategy. Studies have shown the feasibility of directing embryonic stem cells or induced Pluripotent Stem Cells (iPSCs) towards nephrogenic intermediate mesoderm and metanephric mesenchyme (MM). However, the functional activity of iPSC-derived RPCs has not been tested in animal models of kidney disease. Here, through an efficient inductive protocol, we directed human iPSCs towards RPCs that robustly engrafted into damaged tubuli and restored renal function and structure in cisplatin-mice with AKI. These results demonstrate that iPSCs are a valuable source of engraftable cells with regenerative activity for kidney disease and create the basis for future applications in stem cell-based therapy.
Comparison of the Antimanic Efficacy of Carbamazepine and Lithium Carbonate by Double-Blind Controlled Study (1990)

A multi-institutional study comparing the antimanic effect of carbamazepine (CBZ) and lithium carbonate (Li) was performed using a double-blind group comparison design in a series of 105 patients with bipolar disorders. CBZ and Li were given for four weeks using a fixed-flexible method at an equipotent dose ratio of 1:1, starting from an initial dosage of 400 mg with a maximum dosage of 1200 mg. The final global improvement rate, based on the number of cases showing moderate to marked amelioration of manic symptoms, was 62% in the CBZ group and 59% in the Li group, with no significant difference being found between the two groups. Incidence of cutaneous side-effects was significantly higher in the CBZ group. The mean daily dosage and serum level of CBZ in the fourth week were 674 +/- 239 mg and 7.3 +/- 2.4 micrograms/ml respectively; these were within the therapeutic range. The daily dose and serum level of Li, however, were 710 +/- 239 mg and 0.46 +/- 0.22 mEq/l, and the Li level seemed to be too low to compare its therapeutic effect with that of CBZ. Prior to the present study, approximately 80% of the patients in both groups had been receiving antipsychotic medication, equivalent to 8.0 mg of haloperidol on average, without favorable response. This medication was maintained unchanged during treatment. While the shortcomings of the present study limit the interpretation of the data, it may be suggested that the usefulness of CBZ as a drug for the treatment of manic states is comparable to that of Li.
<title>DLP technolgy: applications in optical networking</title> (2001)

For the past five years, Digital Light Processing (DLP) technology from Texas Instruments has made significant inroads in the projection display market. With products encompassing the world's smallest data & video projectors, HDTVs, and digital cinema, DLP is an extremely flexible technology. At the heart of these display solutions is Texas Instruments Digital Micromirror Device (DMD), a semiconductor-based light switch array of thousands of individually addressable, tiltable, mirror-pixels. With success of the DMD as a spatial light modulator in the visible regime, the use of DLP technology under the constraints of coherent, infrared light for optical networking applications is being explored. As a coherent light modulator, the DMD device can be used in Dense Wavelength Division Multiplexed (DWDM) optical networks to dynamically manipulate and shape optical signals. This paper will present the fundamentals of using DLP with coherent wavefronts, discuss inherent advantages of the technology, and present several applications for DLP in dynamic optical networks.
Second quadrant effects in silicon solar cells (1980)

A simple model describing the behavior of a solar cell in the second quadrant of the I-V curve is described. In this mode of operation appreciable power is dissipated in the cell leading to thermal breakdown and the formation of hot spots. These hot spots will be stable in a current limited mode of operation. In addition a simple method is described to locate the hot spots.
A New Mask-To-Wafer Alignment Technique For Synchrotron Radiation X-Ray Lithography (1987)

A new interferometric optical-heterodyne method has been developed for detection of displacement between a mask and a wafer. This method uses three symmetrically-arranged gratings and detects the displacement from the phases of beat signals. Using a 0.76-μm-period-grating system and a He-Ne transverse-mode Zeeman laser( wave length = 0.6328 μm ), sensitivity better than 1° /0.01 μm was obtained and displacement smaller than 5nm was detected independently of the mask-wafer gap variations. With this method, a prototype alignment system having vertical mask and wafer stages was constructed for synchrotron x-ray lithography. The alignment accuracy better than 0.01 μm was achieved. Effects of several factors ( dimension of grating, resist coating, etc. ) influencing the alignment accuracy are discussed.
Constructing Campus Cultural Values and Promoting Soft Power of University (2014)

Campus cultural values, is soul of soft power of university. Cultivating, compacting and constructing campus cultural values are essential requirements for promoting soft power, consolidating Marxist guiding status and forming teachers and students’ common ideal faith, strengthening their patriotism education and forming their common goal purist, and carrying out the education of socialist outlook for honor and dishonor and forming campus civilized new trend. The soft power of university is promoted by deeply excavating and researching historical and cultural remains of university, systematically summarizing and compacting historical and cultural traditions, and energetically inheriting and developing great culture of university. Furthermore, cultural environment including “soft”, “hard”, “virtual” and “realistic” environment that are run through campus cultural values should be powerful built.
973. The synthesis of emetine and related compounds. Part III. The reaction of some isoquinoline derivatives with acrylonitrile (1961)

H. T. Openshaw and N. Whittaker, J. Chem. Soc., 1961, 4939 DOI: 10.1039/JR9610004939
Electrochemical Formation of a Phthalocyanine Thin Film by Disruption of Micellar Aggregates (1988)

Abstract Phthalocyanine (1) was dissolved to the micellar solution formed by non-ionic surfactants with ferrocenyl moiety. A 1 thin film was prepared on an indium tin oxide (ITO) electrode by the controlled-potential electrolysis of this solution. This film consisted of rod-like crystals of 500 nm in length.
High-resolution analysis of epigenetic changes associated with X inactivation (2009)

Differentiation of female murine ES cells triggers silencing of one X chromosome through X-chromosome inactivation (XCI). Immunofluorescence studies showed that soon after Xist RNA coating the inactive X (Xi) undergoes many heterochromatic changes, including the acquisition of H3K27me3. However, the mechanisms that lead to the establishment of heterochromatin remain unclear. We first analyze chromatin changes by ChIP-chip, as well as RNA expression, around the X-inactivation center (Xic) in female and male ES cells, and their day 4 and 10 differentiated derivatives. A dynamic epigenetic landscape is observed within the Xic locus. Tsix repression is accompanied by deposition of H3K27me3 at its promoter during differentiation of both female and male cells. However, only in female cells does an active epigenetic landscape emerge at the Xist locus, concomitant with high Xist expression. Several regions within and around the Xic show unsuspected chromatin changes, and we define a series of unusual loci containing highly enriched H3K27me3. Genome-wide ChIP-seq analyses show a female-specific quantitative increase of H3K27me3 across the X chromosome as XCI proceeds in differentiating female ES cells. Using female ES cells with nonrandom XCI and polymorphic X chromosomes, we demonstrate that this increase is specific to the Xi by allele-specific SNP mapping of the ChIP-seq tags. H3K27me3 becomes evenly associated with the Xi in a chromosome-wide fashion. A selective and robust increase of H3K27me3 and concomitant decrease in H3K4me3 is observed over active genes. This indicates that deposition of H3K27me3 during XCI is tightly associated with the act of silencing of individual genes across the Xi.
When the workplace and social media collide (2013)

Rather than simply banning social media in the office, smart firms should utilise it for the mutual benefit of the business and employees, write Nichola Constant and Misa Han.