gebhart/patrepeval huggingface dataset:
Species-dependent post-translational modification and position 2 allelism: effects on streptococcal superantigen SSA structure and V beta specificity (1996)

Epidemiologic and molecular population genetic analyses support a role for superantigens (SAg) in the pathogenesis of severe staphylococcal and streptococcal infections. To investigate how variations in SAg structure influence immunomodulatory activity, we examined the biochemical and functional properties of two allelic variants of streptococcal SAg SSA that differ at position 2. Mass spectrometry revealed both recombinant (Escherichia coli) and native (Streptococcus pyogenes) SSA allelic variants to have significantly larger molecular masses than predicted by primary sequence alone and provided evidence that the proteins were modified by the addition of biochemical moieties, a phenomenon that has not been described for related SAg. Furthermore, the molecular masses of native and recombinant SSA were not the same; SSA was differentially post-translationally modified by the two bacterial genera. The substitution of E. coli-dependent processing for that of S. pyogenes altered both protease digestion and V beta specificity, suggesting that recombinant SAg from E. coli may not accurately represent the native toxin. In addition, the observation that SSA allelic variants differed in V beta specificity supports a role for position 2 in SSA-TCR interactions. That SSA position 2 contributes to V beta specificity could not have been predicted from functional or crystallographic studies of other SAg and suggests that SSA may adopt unique interactions with TCR and/or MHC class II molecules. Determining the structural basis for these differences should offer additional clues to the manner in which SAg exert their effects on the immune system during infection and may allow the designing of SAg mutants with specific quantitative and qualitative immunomodulatory properties.
Solution of Large-Scale Optimal Unit Commitment Problems (1982)

This paper is concerned with the solution of large-scale unit commitment problems. An optimization model has been developed for these problems that incorporates minimum up and down time constraints, demand and reserve constraints, cooling-time dependent startup-costs, and time varying shutdown costs, as well as other practical considerations. A solution methodology has been developed for the optimization model that has two unique features. First, computational requirements grow only linearly with the number of units. Second, performance of the algorithm can be shown (rigorously) to actually improve as the number of units increases. With a preliminary computer implementation of the algorithm, we have been able to reliably solve problems with 250 units over 12 (2-hour) time periods, and we expect to be able to easily double these numbers.
Self-Assembly of a Diblock Copolymer on a Patterned Surface with Low-Energy Electron Beam (2006)

A pattern was generated by 500 eV electron beam irradiation on benzaldimine monolayer through a grid and subsequent hydrolysis of nonirradiated regions. While we tried to assemble a block copolymer, polystyrene-block-poly(4-vinylpyridine) (PS-b-P4VP), on the pattern, we observed that the polarity difference between the two different regions was not right for discriminating the wetting behavior of two blocks of the polymer. Among various modifications of the retrieved amine, it was found that tribromoacetaldehyde was suitable for this end. Surprisingly, treatment of the aldehyde gave a surface preferring the polystyrene block to poly(4-vinylpyridine) block, while the irradiated section favored the latter block. As a result, island morphology was observed on the tribromoacetaldimine region and hole morphology on the irradiated region when the film thickness was 1.3Lo. Contact angle data were consistent with the observed symmetric wetting on the former region and the asymmetric one on the latter.
Immune Responses Induced by Replication-Defective Adenovirus Expressing the C-Terminal Portion of the<i>Mycoplasma hyopneumoniae</i>P97 Adhesin (2007)

Mycoplasma hyopneumoniae, the causative agent of porcine enzootic pneumonia, colonizes the respiratory cilia of affected swine, causing significant economic losses to swine production worldwide. Vaccination is the most cost-effective strategy for the control and prevention of this disease. The goal of this study was to design and evaluate a replication-defective recombinant adenovirus, rAdP97c, expressing the C-terminal portion of P97 adhesin (P97c), an important pathogenesis-associated protein of M. hyopneumoniae, as a new vaccine candidate against M. hyopneumoniae infection. P97c-specific immune responses were evaluated in BALB/c mice following intranasal and intramuscular inoculation with rAdP97c. Mice inoculated by both routes of immunization produced significant levels of specific immunoglobulin G (IgG) antibodies in the serum and in bronchoalveolar lavage fluids (BALs). Animals immunized intranasally also produced a significant level of P97c-specific IgA in BALs. Intramuscular inoculation of rAdP97c induced a systemic and mucosal Th1-type biased response, evidenced by the predominance of IgG2a in the serum and BALs, whereas intranasal inoculation resulted in a mixed Th1/Th2-type response (balanced levels of IgG1 and IgG2a) in both sytemic and mucosal compartments. P97c-specific antibodies were able to inhibit the growth of M. hyopneumoniae cells in vitro. These data suggest that rAdP97c vaccine may represent a new strategy for controlling infection by M. hyopneumoniae.
Astrocytes are central in the pathomechanisms of vanishing white matter (2016)

Vanishing white matter (VWM) is a fatal leukodystrophy that is caused by mutations in genes encoding subunits of eukaryotic translation initiation factor 2B (eIF2B). Disease onset and severity are codetermined by genotype. White matter astrocytes and oligodendrocytes are almost exclusively affected; however, the mechanisms of VWM development remain unclear. Here, we used VWM mouse models, patients' tissue, and cell cultures to investigate whether astrocytes or oligodendrocytes are the primary affected cell type. We generated 2 mouse models with mutations (Eif2b5Arg191His/Arg191His and Eif2b4Arg484Trp/Arg484Trp) that cause severe VWM in humans and then crossed these strains to develop mice with various mutation combinations. Phenotypic severity was highly variable and dependent on genotype, reproducing the clinical spectrum of human VWM. In all mutant strains, impaired maturation of white matter astrocytes preceded onset and paralleled disease severity and progression. Bergmann glia and retinal Müller cells, nonforebrain astrocytes that have not been associated with VWM, were also affected, and involvement of these cells was confirmed in VWM patients. In coculture, VWM astrocytes secreted factors that inhibited oligodendrocyte maturation, whereas WT astrocytes allowed normal maturation of VWM oligodendrocytes. These studies demonstrate that astrocytes are central in VWM pathomechanisms and constitute potential therapeutic targets. Importantly, astrocytes should also be considered in the pathophysiology of other white matter disorders.
Autologous Hematopoietic Stem-Cell Transplantation for Children With Acute Myeloid Leukemia in First or Second Complete Remission: A Prognostic Factor Analysis (2004)

Duration of first CR seems to be the most important determinant of outcome. Results in children who experience treatment failure with conventional chemotherapy support the use of autologous transplantation as salvage therapy if such patients achieve a subsequent CR.
Learning from Source Code History to Identify Performance Failures (2016)

Source code changes may inadvertently introduce performance regressions. Benchmarking each software version is traditionally employed to identify performance regressions. Although effective, this exhaustive approach is hard to carry out in practice. This paper contrasts source code changes against performance variations. By analyzing 1,288 software versions from 17 open source projects, we identified 10 source code changes leading to a performance variation (improvement or regression). We have produced a cost model to infer whether a software commit introduces a performance variation by analyzing the source code and sampling the execution of a few versions. By profiling the execution of only 17% of the versions, our model is able to identify 83% of the performance regressions greater than 5% and 100% of the regressions greater than 50%.
A heterotrimeric G protein complex couples the muscarinic m1 receptor to phospholipase C-beta. (1996)

We addressed the question as to which subtypes of G protein subunits mediate the activation of phospholipase C-beta by the muscarinic m1 receptor. We used the rat basophilic leukemia cell line RBL-2H3-hm1 stably transfected with the human muscarinic m1 receptor cDNA. We microinjected antisense oligonucleotides into the nuclei of the cells to inhibit selectively the expression of G protein subunits; 48 hr later muscarinic receptors were activated by carbachol, and the increase in free cytosolic calcium concentration ([Ca2+]i) was measured. Antisense oligonucleotides directed against the mRNA coding for alpha(q) and alpha11 subunits both suppressed the carbachol-induced increase in [Ca2+]i. In cells injected with antisense oligonucleotides directed against alpha(o1) and alpha14 subunits, the carbachol effect was unchanged. A corresponding reduction of Galpha(q), and Galpha11 proteins by 70-80% compared to uninjected cells was immunochemically detected 2 days after injection of a mixture of alpha(q) and alpha11 antisense oligonucleotides. Expression of Galpha(q) and Galpha11 completely recovered after 4 days. Cells injected with antisense oligonucleotides directed against the mRNAs encoding for beta1, beta4, and gamma4 subunits showed a suppression of the carbachol-induced increase in [Ca2+]i compared to uninjected cells measured at the same time from the same coverslip, whereas in cells injected with antisense oligonucleotides directed against the beta2, beta3, gamma1, gamma2, gamma3, gamma5, and gamma7 subunits, no suppression of carbachol effect was observed. In summary, the results from RBL-2H3-hm1 cells indicate that the m1 receptor utilizes a G protein complex composed of the subunits alpha(q), alpha11, beta1, beta4, and gamma4 to activate phospholipase C.
Substance P and Affective Disorders: New Treatment Opportunities by Neurokinin 1 Receptor Antagonists? (2002)

Substance P (SP) is a neuropeptide which is abundant in the periphery and the central nervous system, where it is colocalized with other neurotransmitters such as serotonin or dopamine. SP has been proposed to play a role in the regulation of pain including migraine and fibromyalgia, asthma, inflammatory bowel disease, emesis, psoriasis as well as in central nervous system disorders. This review summarizes our current knowledge of the role of SP in the pathogenesis of neuropsychiatric disorders with special emphasis on affective disorders including bipolar disorders. It also reviews current treatment approaches with neurokinin 1 receptor antagonists which appear to be promising drugs for the future treatment of affective disorders.
Increased In-Shoe Lateral Plantar Pressures with Chronic Ankle Instability (2011)

These findings may have implications in the etiology and treatment of chronic ankle instability.
Topologically protected elastic waves in phononic metamaterials (2015)

Surface waves in topological states of quantum matter exhibit unique protection from backscattering induced by disorders, making them ideal carriers for both classical and quantum information. Topological matters for electrons and photons are largely limited by the range of bulk properties, and the associated performance trade-offs. In contrast, phononic metamaterials provide access to a much wider range of material properties. Here we demonstrate numerically a phononic topological metamaterial in an elastic-wave analogue of the quantum spin Hall effect. A dual-scale phononic crystal slab is used to support two effective spins for phonons over a broad bandwidth, and strong spin-orbit coupling is realized by breaking spatial mirror symmetry. By preserving the spin polarization with an external load or spatial symmetry, phononic edge states are shown to be robust against scattering from discrete defects as well as disorders in the continuum, demonstrating topological protection for phonons in both static and time-dependent regimes.
June UK Council (2004)

Council held its third meeting of the year on Saturday 19 June. As usual, I am writing to let you know the main areas of discussion and some of the decisions we reached at the meeting.

Dr Mayur Lakhani was formally announced as Chairman elect to take over from Professor David Haslam in November. A number of other posts in networks and committees will become vacant in November when the present Chairs complete their term of office. Full details of all posts will be coming to Council in September and an election for these posts will take place as soon as possible following the September meeting. I hope a number of Council members will take the …
Supporting social presence through lightweight photo sharing on and off the desktop (2004)

Lightweight photo sharing, particularly via mobile devices, is fast becoming a common communication medium used for maintaining a presence in the lives of friends and family. How should such systems be designed to maximize this social presence while maintaining simplicity? An experimental photo sharing system was developed and tested that, compared to current systems, offers highly simplified, group-centric sharing, automatic and persistent people-centric organization, and tightly integrated desktop and mobile sharing and viewing. In an experimental field study, the photo sharing behaviors of groups of family or friends were studied using their normal photo sharing methods and with the prototype sharing system. Results showed that users found photo sharing easier and more fun, shared more photos, and had an enhanced sense of social presence when sharing with the experimental system. Results are discussed in the context of design principles for the rapidly increasing number of lightweight photo sharing systems.
High β-Glucosidase Secretion in Saccharomyces cerevisiae Improves the Efficiency of Cellulase Hydrolysis and Ethanol Production in Simultaneous Saccharification and Fermentation (2013)

Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this study, three β-glucosidases from different organisms were respectively expressed in Saccharomyces cerevisiae and the β-glucosidase from Saccharomycopsis fibuligera showed the best activity (5.2 U/ml). The recombinant strain with S. fibuligera β-glucosidase could metabolize cellobiose with a specific growth rate similar to the control strain in glucose. This recombinant strain showed higher hydrolysis efficiency in the cellulose simultaneous saccharification and fermentation, when using the Trichoderma reesei cellulase, which is short of the β-glucosidase activity. The final ethanol concentration was 110% (using Avicel) and 89% (using acid-pretreated corncob) higher than the control strain. These results demonstrated the effect of β-glucosidase secretion in the recombinant S. cerevisiae for enhancing cellulosic ethanol conversion.
The Endothelial Glycocalyx: Visualization and Measurement (2017)

The endothelial glycocalyx is a hair-like surface layer mainly consisted of glycosaminoglycans (GAGs) that includes heparan sulfate (HS), chondroitin sulfate (CS), and hyaluronic acid (HA) and its bearing core proteins (syndecan, glypican, and perlecan). This mini review presents the visualization and measurement techniques of the endothelial glycocalyx up to now, providing insight to the diagnosis, treatment, and protection of the cardiovascular disease.
Studies on the carotenoid pigments of paprika (Capsicum annuum L.var Sz-20) (1989)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on the carotenoid pigments of paprika (Capsicum annuum L.var Sz-20)Peter A. Biacs, Hussein G. Daood, Anna Pavisa, and Felix HajduCite this: J. Agric. Food Chem. 1989, 37, 2, 350–353Publication Date (Print):March 1, 1989Publication History Published online1 May 2002Published inissue 1 March 1989https://pubs.acs.org/doi/10.1021/jf00086a017https://doi.org/10.1021/jf00086a017research-articleACS PublicationsRequest reuse permissionsArticle Views810Altmetric-Citations84LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Computer-assisted trajectory planning for percutaneous needle insertions (2011)

The proposed computer-assisted trajectory planning system is able to detect unsafe and propose safe insertion trajectories and may especially be helpful for interventional radiologist at the beginning or during their interventional training.
Low-noise, broadband Er <sup>3+</sup> -doped silicafibre amplifiers (1998)

A broadband, low-noise silica-based erbium-doped fibre amplifier with a wide amplification bandwidth of 70 nm and a low noise figure of < 5 dB has been developed, by employing a hybrid configuration and adjusting the length of the Er3+-doped silica fibres used in the amplifier.
Facile Synthesis of 2-Bromo-3-fluorobenzonitrile:  An Application and Study of the Halodeboronation of Aryl Boronic Acids (2003)

A scaleable synthesis of 2-bromo-3-fluorobenzonitrile via the NaOMe-catalyzed bromodeboronation of 2-cyano-6-fluorophenylboronic acid was developed. The generality of this transformation was demonstrated through the halodeboronation of a series of aryl boronic acids. Both aryl bromides and aryl chlorides were formed in good to excellent yields when the corresponding aryl boronic acid was treated with 1,3-dihalo-5,5-dimethylhydantoin and 5 mol % NaOMe.
Ueber die Verbindungen der Elemente der Stickstoffgruppe mit den Radicalen der aromatischen Reihe (1876)

phenylirtr Phosphorchlorur , PC6H&12 , addirt dagegen eben so leicht Brom t i e Phosphorchloriir Chtor und diese Verbindung ist so bestiindig,
Lensless Diffractive Imaging Using Tabletop Coherent High-Harmonic Soft-X-Ray Beams (2007)

We present the first experimental demonstration of lensless diffractive imaging using coherent soft x rays generated by a tabletop soft-x-ray source. A 29 nm high harmonic beam illuminates an object, and the subsequent diffraction is collected on an x-ray CCD camera. High dynamic range diffraction patterns are obtained by taking multiple exposures while blocking small-angle diffraction using beam blocks of varying size. These patterns reconstruct to images with 214 nm resolution. This work demonstrates a practical tabletop lensless microscope that promises to find applications in materials science, nanoscience, and biology.
The Use of Substituted Phenols in the Mannich Reaction and the Dehalogenation of Aminomethylhalophenols (1959)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Use of Substituted Phenols in the Mannich Reaction and the Dehalogenation of AminomethylhalophenolsF. F. BLICKE and F. J. McCARTYCite this: J. Org. Chem. 1959, 24, 8, 1061–1069Publication Date (Print):August 1, 1959Publication History Published online1 May 2002Published inissue 1 August 1959https://pubs.acs.org/doi/10.1021/jo01090a008https://doi.org/10.1021/jo01090a008research-articleACS PublicationsRequest reuse permissionsArticle Views1220Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
DOSIMETRY BASED ON EPR SPECTRAL ANALYSIS OF FINGERNAIL CLIPPINGS (2010)

Exposure of fingernails and toenails to ionizing radiation creates radicals that are stable over a relatively long period (days to weeks) and characterized by an isotropic EPR signal at g = 2.003 (so-called radiation-induced signal, RIS). This signal in readily obtained fingernail parings has the potential to be used in screening a population for exposure to radiation and determining individual dose to guide medical treatment. However, the mechanical harvesting of fingernail parings also creates radicals, and their EPR signals (so-called mechanically-induced signals, MIS) overlap the g approximately 2.0 region, interfering with efforts to quantify the RIS and, therefore, the radiation dose. Careful analysis of the time evolution and power-dependence of the EPR spectra of freshly cut fingernail parings has now resolved the MIS into three major components, including one that is described for the first time. It dominates the MIS soon after cutting, but decays within the first hour and consists of a unique doublet that can be resolved from the RIS. The MIS obtained within the first few minutes after cutting is consistent among fingernail samples and provides an opportunity to achieve the two important dosimetry objectives. First, perturbation of the initial MIS by the presence of RIS in fingernails that have received a threshold dose of radiation leads to spectral signatures that can be used for rapid screening. Second, decomposition of the EPR spectra from irradiated fingernails into MIS and RIS components can be used to isolate and thus quantify the RIS for determining individual exposure dose.
GONADOTROPHIC ACTIVITY IN THE PITUITARY AND URINE OF THE MALE RABBIT AFTER OXYTOCIN TREATMENT (1971)

ABSTRACT The effects of treatment with synthetic oxytocin on the urinary and pituitary gonadotrophins in male rabbits were evaluated. Oxytocin was injected twice (2 × 1 IU per kg body weight) every third day for a period of 18 days. The amounts of total urinary gonadotrophins were significantly increased for 1–3 days after each day of oxytocin injection. The total pituitary gonadotrophins and FSH were reduced to about 20% of the control values, 2 hours after the last of the oxytocin injections.