gebhart/patrepeval huggingface dataset:
Functional Properties of a <i>Drosophila</i> Homolog of the E2F1 Gene (1994)

A variety of studies have now implicated the cellular transcription factor E2F as a key participant in transcription control during the cell growth cycle. Although the recent isolation of molecular clones encoding proteins that are components of the E2F activity (E2F1 and DP-1) provides an approach to defining the specific involvement of E2F in these events, definitive experiments remain difficult in the absence of appropriate genetic systems. We have now identified a Drosophila equivalent of E2F1 that we hope will allow an eventual genetic approach to the role of E2F in cellular regulatory events. A cDNA clone was isolated from a Drosophila cDNA library by using a probe containing sequence from the E2F1 DNA binding domain. The sequence of the clone, which we term drosE2F1, demonstrates considerable homology to the human E2F1 sequence, with over 65% identity in the DNA binding region and 50% identity in the region of E2F1 known to interact with the retinoblastoma gene product. A glutathione S-transferase-drosE2F1 fusion protein was capable of binding specifically to an E2F recognition site, and transfection assays demonstrated that the drosE2F1 product was capable of transcription activation, dependent on functional E2F sites as well as sequences within the C terminus of the protein. Finally, we have also identified E2F recognition sequences within the promoter of the Drosophila DNA polymerase alpha gene, and we demonstrate that the drosE2F1 product activates transcription of a test gene under the control of this promoter. We conclude that the drosE2F1 cDNA encodes an activity with extensive structural and functional similarity to the human E2F1 protein.
Inorganic environmental analysis by capillary electrophoresis (1999)

A. R. Timerbaev, E. Dabek-Zlotorzynska and M. A. G. T. van den Hoop, Analyst, 1999, 124, 811 DOI: 10.1039/A901059I
Blood glucose monitoring using microwave cavity perturbation (2012)

An outline is presented of the design and preliminary tests of a method for tracking blood glucose levels using dielectric spectroscopy. The system designed uses a microwave cavity to measure the concentration of aqueous solutions of glucose, as a change in complex permittivity. The results of this research are very promising, finding frequency regions where only a single frequency needs to be applied to measure a change, allowing easy implementation of a practical system.
Analysis of ripple size evolution in the LT process (2018)

This study introduces a novel framework and analysis tool to monitor the evolution of the ripple size during the Luby‐transform (LT) decoding process. It provides a closed‐form probability expression to statistically describe the behaviour of the ripple size at each step of the decoding. The probability function is conditioned on the size of the ripple in the previous step, the number of recovered source symbols and the encoded symbols' degree distribution. The authors further derive a closed‐form expression for the ripple size after new encoded symbols are received by a decoder with an empty ripple. This relates to the activation of belief propagation decoding attempts once premature terminations occur. They validate their analyses through numerical examples of an LT‐coded system. The results show that their analyses precisely coincide with the numerical results.
Vermicompost and farmyard manure effects on biogeochemical performances of Withania somnífera (2011)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
A Novel Frequency Tuning System Based on Movable Plunger for SPIRAL2 High-Beta Superconducting Quarter-Wave Resonator (2008)

SPIRAL2 aims at building a multi-purpose facility dedicated to nuclear physics studies, including the production of rich-neutrons isotopes. The multi-beam linear accelerator is composed of superconducting accelerating modules and warm focusing magnets. IPN Orsay is in charge of the high energy accelerating modules, each hosting two superconducting 88 MHz quarter-wave resonators operating at an accelerating field of 6.5 MV/m (β = 0.12). The static and dynamic frequency tuning is achieved by the insertion and displacement of a niobium plunger into the magnetic field area. The efficiency of the tuning (1 kHz/mm) has been validated during the tests of the cryomodule. In this paper we discuss the impact of such a tuning system, based on experimental results on SPIRAL2 cavities, on the different aspects: maximum accelerating field, Qo slopes, quench, multipacting and microphonics. INTRODUCTION The multi-purpose linear accelerator for SPIRAL2 has entered the construction phase since April 2006 [1]. Niobium quarter-wave resonators, composing the high energy part of the linac, have already been prototyped and qualified thanks to the construction of 2 cavities. These high-beta (β = 0.12) cavities resonating at 88 MHz, contrary to the low-beta ones (β = 0.07), are not tuned with a classical wall-deformation system due to the too low frequency shift versus the force applied [2]. The solution retained is the frequency tuning with a niobium movable plunger inserted into the magnetic field part of the cavity. This paper will present the full mechanism in the first part and then discuss the different results obtained with electromagnetic simulations and tests.
Site‐specific <i>N</i> ‐glycosylation analysis: matrix‐assisted laser desorption/ionization quadrupole‐quadrupole time‐of‐flight tandem mass spectral signatures for recognition and identification of glycopeptides (2004)

Abstract The identification of glycosylation sites in proteins is often possible through a combination of proteolytic digestion, separation, mass spectrometry (MS) and tandem MS (MS/MS). Liquid chromatography (LC) in combination with MS/MS has been a reliable method for detecting glycopeptides in digestion mixtures, and for assigning glycosylation sites and glycopeptide sequences. Direct interfacing of LC with MS relies on electrospray ionization, which produces ions with two, three or four charges for most proteolytic peptides and glycopeptides. MS/MS spectra of such glycopeptide ions often lead to ambiguous interpretation if deconvolution to the singly charged level is not used. In contrast, the matrix‐assisted laser desorption/ionization (MALDI) technique usually produces singly charged peptide and glycopeptide ions. These ions require an extended m/z range, as provided by the quadrupole‐quadrupole time‐of‐flight (QqTOF) instrument used in these experiments, but the main advantages of studying singly charged ions are the simplicity and consistency of the MS/MS spectra. A first aim of the present study is to develop methods to recognize and use glycopeptide [M+H] + ions as precursors for MS/MS, and thus for glycopeptide/glycoprotein identification as part of wider proteomics studies. Secondly, this article aims at demonstrating the usefulness of MALDI‐MS/MS spectra of N ‐glycopeptides. Mixtures of diverse types of proteins, obtained commercially, were prepared and subjected to reduction, alkylation and tryptic digestion. Micro‐column reversed‐phase separation allowed deposition of several fractions on MALDI plates, followed by MS and MS/MS analysis of all peptides. Glycopeptide fractions were identified after MS by their specific m/z spacing patterns (162, 203, 291 u) between glycoforms, and then analyzed by MS/MS. In most cases, MS/MS spectra of [M+H] + ions of glycopeptides featured peaks useful for determining sugar composition, peptide sequence, and thus probable glycosylation site. Peptide‐related product ions could be used in database search procedures and allowed the identification of the glycoproteins. Copyright © 2004 John Wiley & Sons, Ltd.
A comparator for materials of low magnetic susceptibility (1954)

This paper describes an instrument for comparing the magnetic states of weakly magnetic materials, such as phosphor bronze, copper, filled rubber, etc. The force generated between a suspended magnet and the sample under test causes deflexion of a light beam. The instrument, as used at present, is suitable for comparisons in the range μ = 1 ± 0.00001 to μ = 1 ± 0.0002, but it can be adjusted to cover other values of μ. It has been found particularly useful for detecting magnetic contamination in fine gauge copper wire for instrument work.
Requirements of a coherent laser pulse-Doppler radar (1963)

The use of coherent detection can theoretically allow optical radar systems employing laser transmitters to achieve considerably improved receiver sensitivity, particularly in conditions of high background radiation. However, there are many practical factors that can limit sensitivity in coherent optical detection, which are described. It is shown that to achieve an efficient coherent optical radar, one would generally like a pulse width less than 10 µsec and a spectral line width less than 10 Mc.
A virtual 3D blackboard: 3D finger tracking using a single camera (2002)

We present a method for tracking the 3D position of a finger, using a single camera placed several meters away from the user. After skin detection, we use motion to identify the gesticulating arm. The finger point is found by analyzing the arm's outline. To derive a 3D trajectory, we first track 2D positions of the user's elbow and shoulder. Given that a human's upper arm and lower arm have consistent length, we observe that the possible locations of a finger and elbow form two spheres with constant radii. From the previously tracked body points, we can reconstruct these spheres, computing the 3D position of the elbow and finger. These steps are fully automated and do not require human intervention. The system presented can be used as a visualization tool, or as a user input interface, in cases when the user would rather not be constrained by the camera system.
Evaluation of 16 SNPs allele-specific to quantify post hSCT chimerism by SYBR green-based qRT-PCR (2013)

The importance of monitoring post haematopoietic stem cell transplantation (hSCT) chimerism has been defined in numerous publications. Single-nucleotide polymorphisms (SNPs) are molecular markers that vary significantly among different populations. Allied to a very sensible technique, SNP assays seem to be very sensitive (0.001%) when post hSCT chimerism is measured. However, well known SNP frequencies are limited to certain populations, mainly in countries where there is a high level of diversity in its population, therefore restricting their use worldwide. Amplification by SYBR green based quantitative real time PCR of eight pairs of allele-specific SNPs (MLH-1, PECAM-1, ICAM-1, SUR-1, HA-1, rs715405, rs713503, rs2296600) was conducted in 88 patient/donor pairs, who underwent allogeneic myeloablative or non-myeloablative hSCT. One informative allele was detected in at least 42% (n=37) of the samples; 20% (n=18) had at least two informative alleles; 10% (n=9) had at least three informative alleles; 9% (n=8) had more than three informative alleles and 18% (n=16) showed no informative allele at all. Overall, the frequency of informative alleles for these SNPs in the Brazilian population was very low. Consequently, the amount of information attained reached 9% of those expected, being able to discriminate only eight pairs of donor/recipient samples with more than three informative alleles, making them useless for the quantification of chimerism in our routine.
IL-10 suppresses bactericidal response of macrophages against Salmonella Typhimurium (2011)

We report, herein, an attempt to determine whether an IL-10-induced immunological state affects the response of macrophages against Salmonella Typhimurium (ST). Pretreatment with mrIL-10 induced the intracellular invasion of ST into macrophages in a dose-dependent manner. It also activated AKT phosphorylation, cyclin D1, Bcl-X(L), and COX-2 upon ST infection, which may correlate with Salmonella's survival within the macrophages. However, I-κB phosphorylation was shown to be inhibited, along with the expression of TNF-α and MIP-2α mRNA. Therefore, IL-10 not only suppresses the bactericidal response of macrophages against ST, but also ultimately causes infected macrophages to function as hosts for ST replication.
Long-Term Safety of Remoxy<sup>®</sup>(Extended-Release Oxycodone) in Patients with Moderate to Severe Chronic Osteoarthritis or Low Back Pain (2011)

Objective. Evaluate the long-term safety, tolerability, and efficacy of Remoxy® (extended-release oxycodone) in patients with chronic pain related to osteoarthritis of the hip and/or knee or chronic low back pain.
Botulinum toxin enhancement of postoperative immobilization in patients with cervical dystonia (1992)

Postoperative immobilization in patients with cervical dystonia requiring fusion presents a unique management problem. Two patients with severe degenerative cervical spine disease secondary to chronic repetitive motion are reported. Both required a surgical fusion and postoperative immobilization. Botulinum toxin was injected intramuscularly to assist in immobilization. The technique used is described.
Text prediction for translators (2002)

Demand for the services of translators is on the increase, and consequently so is the demand for tools to help them improve their productivity. This thesis proposes a novel tool intended to give a translator interactive access to the most powerful translation technology available: a machine translation system. The main new idea is to use the target text being produced as the medium of interaction with the computer. In contrast to previous approaches, this is natural and flexible, placing the translator in full control of the translation process, but giving the tool scope to contribute when it can usefully do so. 
A simple version of this idea is a system that tries to predict target text in real time as a translator types. This can aid by speeding typing and suggesting ideas, but it can also hinder by distracting the translator, as previous studies have demonstrated. I present a new method for text prediction that aims explicitly at maximizing a translator's productivity according to a model of user characteristics. Simulations show that this approach has the potential to improve the productivity of an average translator by over 10%. 
The core of the text prediction method presented here is the statistical model used to estimate the probability of upcoming text. This must be as accurate as possible, but also efficient enough to support real-time searching. I describe new models based on the technique of maximum entropy that are specifically designed to balance accuracy and efficiency for the prediction application. These outperform equivalent baseline models used in prior work by about 50% according to an empirical measure of predictive accuracy, with no sacrifice in efficiency.
Molecular mechanisms underlying lymphocyte recirculation I. Functional, phenotypical and morphological characterization of high endothelial cells culturedin vitro (1988)

Large-scale extravasation of lymphocytes takes place in vivo under physiological conditions in lymph nodes at very specialized vascular segments called high endothelial venules (HEV). When circulating lymphocytes leave the blood, they first bind to endothelial cells of HEV (HE cells) and subsequently enter lymph nodes by crossing the endothelial lining of HEV. Although the lymphocyte-HEV interaction has recently been the subject of intense research by many laboratories, it has been studied almost exclusively by the use of the lymphocyte-binding assay in which lymphocyte binding is examined on nonviable HEV present on frozen sections and, hence, no dynamic interaction between HE cells and lymphocytes has been studied. We report herein that endothelial cells of rat HEV can be grown in vitro and that the lymphocyte-HEV interaction can be studied dynamically using viable cells in culture vessels. The identification of the cultured line, termed Ax, as HE cells was based on their phenotypic, morphological, cytochemical and biochemical characteristics, and most importantly on its in vitro behavior, particularly in terms of its specific ability to interact with mature lymphocytes. Phenotypic analysis demonstrated that not only did monoclonal antibodies, known to react with HE cells, recognize the Ax but also a monoclonal antibody raised against the Ax specifically recognized HE cells in vivo, as determined by an immunoperoxidase staining of frozen sections, supporting the notion that the cell strain, Ax, is derived from HEV. This Ax, even after long-term culture (greater than 50 passages), allowed mature, but not immature, lymphocytes to bind to the cell surface and subsequently transport bound cells underneath their cytoplasm. This phenomenon was inhibited in a dose-dependent manner by various reagents known to inhibit lymphocyte recirculation in vivo. The cultured line derived from HE cells should provide a means to investigate the biochemical nature of lymphocyte-HEV interaction, and to understand the molecular mechanisms underlying the large-scale lymphocyte traffic taking place in vivo.
Alkoxy enediolates (1977)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAlkoxy enediolatesLouis J. Ciochetto, David E. Bergbreiter, and Martin NewcombCite this: J. Org. Chem. 1977, 42, 17, 2948–2950Publication Date (Print):August 1, 1977Publication History Published online1 May 2002Published inissue 1 August 1977https://pubs.acs.org/doi/10.1021/jo00437a044https://doi.org/10.1021/jo00437a044research-articleACS PublicationsRequest reuse permissionsArticle Views218Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Vision-guided robotic leaf picking (2018)

The key focus of precision agriculture is the ability to monitor the health of individual plants. While visual monitoring was made easier by incorporating autonomous ground and aerial vehicles, regular sampling of leaves and soil to perform chemical analysis is still necessary. The focus of this work is to propose a leaf sampling system that uses a monocular camera and a 6-DOF robot arm to detect, track, and pick healthy and unhealthy leaves without prior knowledge about the plant. The approach focuses on the challenges of operating in the real world with the aim of increasing the robustness of the system.
Acute Humoral Rejection of Renal Allografts in CCR5–/– Recipients (2008)

Increasing detection of acute humoral rejection (AHR) of renal allografts has generated the need for appropriate animal models to investigate underlying mechanisms. Murine recipients lacking the chemokine receptor CCR5 reject cardiac allografts with marked C3d deposition in the parenchymal capillaries and high serum donor-reactive antibody titers, features consistent with AHR. The rejection of MHC-mismatched renal allografts from A/J (H-2(a)) donors by B6.CCR5(-/-) (H-2(b)) recipients was investigated. A/J renal allografts survived longer than 100 days in wild-type C57BL/6 recipients with normal blood creatinine levels (28 +/- 7 micromol/L). All CCR5(-/-) recipients rejected renal allografts within 21 days posttransplant (mean 13.3 +/- 4 days) with elevated creatinine (90 +/- 31 micromol/L). The rejected allografts had neutrophil and macrophage margination and diffuse C3d deposition in peritubular capillaries, interstitial hemorrhage and edema, and glomerular fibrin deposition. Circulating donor-reactive antibody titers were 40-fold higher in B6.CCR5(-/-) versus wild-type recipients. Depletion of recipient CD8 T cells did not circumvent rejection of the renal allografts by CCR5-deficient recipients. In contrast, microMT(-/-)/CCR5(-/-) recipients, incapable of producing antibody, did not reject most renal allografts. Collectively, these results indicate the rapid rejection of renal allografts in CCR5(-/-) recipients with many histopathologic features observed during AHR of human renal allografts.
Advanced ANS COBOL with structured programming (1977)

Introduction. Criteria. General Language Rules. Basic COBOL statements. Structured Programming. Numeric Data. Character Data. Data Descriptions and Records. Tables. System/37O Job Control Language. Program Organization. Input/Output Concepts and Devices. Sequential Input/Output. Printed Output. Report Writer. Sorting. Relative. Direct, and Indexed Files. Communications Facility. Beyond COBOL. Appendixes. Index.
The human embryo produces basement membrane collagen (type IV collagen)-degrading protease activity (1989)

The biochemical basis and mechanism of embryo implantation is poorly understood. The human embryo has to penetrate the endometrial basement membrane and the thick decidual wall during implantation, a process which resembles the active spreading of invasive tumour cells where the degradation of type IV collagen (basement membrane collagen) plays an important role. This study reports that human embryos produce type IV collagen-degrading enzyme activity and the secretion of this enzyme increases with time in culture. The type IV collagen-degrading enzyme activity might facilitate the penetration of the embryo through the decidua, thus emphasizing an active role of the embryo in implantation. On the other hand, unfertilized oocytes secrete low, stable amounts of type IV collagen-degrading enzyme activity in vitro. It was also found that follicular granulosa cells secrete high amounts of type IV collagenolytic activity in culture. It has been previously shown that there is a pre-ovulatory peak in type IV collagenolytic activity in follicular fluid, and it can be assumed that the appearance of this enzyme in the follicular fluid is probably connected to follicular rupture and that it is produced by granulosa cells.
Chemical modification of peptides by hydrazine (1981)

High pressure ('performance') liquid chromatography on reverse-phase supports has been used to characterize the products arising from the hydrazine treatment of peptides. In addition to converting arginine residues into ornithine, the reaction was found to cleave predominately Gly-Xaa, Xaa-Gly, Asn-Xaa and Xaa-Ser peptide bonds. Peptide-bond cleavage and deguanidation was studied as a function of time of exposure to hydrazine, hydrazine concentration and temperature. The convenience of this method of chromatography for the rapid low-cost separation and isolation of peptides, as well as their reaction products, is illustrated at the level of material required for solid-phase microsequencing.