gebhart/patrepeval huggingface dataset:
Stimulation of Transcription in Cultured Cells by High Mobility Group Protein 1: Essential Role of the Acidic Carboxyl-Terminal Region (1994)

Several in vitro studies have suggested that high mobility group (HMG) protein 1 has a role in gene regulation as a trans activator or quasi-transcription factor. However, data on the molecular functions of HMG1 protein in these reactions are contradictory or obscure. In order to assess whether HMG1 protein does, in fact, have transcriptional activation potential, two assay systems in cultured cells were employed. HMG1 protein introduced into COS-1 cells as a complex with a reporter plasmid carrying the lacZ gene enhanced the level of the gene expression. Cotransfection of an expression plasmid carrying HMG1 cDNA into the cells with the reporter plasmid enhanced the activity of beta-galactosidase 2-3-fold in comparison with that of the control effector plasmid. The enhancement was proved to be dependent not on the replication but on the transcription of the reporter plasmid. In the cotransfection experiments, an expression plasmid the HMG1 molecule lacking the acidic carboxyl terminus repressed the expression of the reporter gene. The binding of an HMG1 protein variant lacking the acidic carboxyl terminus to DNA gave an extremely large shift of gel retardation in comparison with the complete HMG1 molecule. Together, these results indicate that HMG1 protein can enhance expression in cells in culture at the step of gene transcription and that the DNA binding domains comprising two-thirds of the HMG1 protein molecule are responsible for the inhibition property. Also, the acidic terminus of the HMG1 molecule is essential for the enhancement of gene expression in addition to elimination of the repression caused by the DNA binding. (ABSTRACT TRUNCATED AT 250 WORDS)
HLPR Chair: A Novel Indoor Mobility-Assist and Lift System (2007)

This paper describes a novel Home Lift, Position, and Rehabilitation (HLPR) Chair, designed at National Institute of Standards and Technology (NIST), to provide independent patient mobility for indoor tasks, such as moving to and placing a person on a toilet or bed, and lift assistance for tasks, such as accessing kitchen or other tall shelves. These functionalities are currently out of reach of most wheelchair users. One of the design motivations of the HLPR Chair is to reduce back injury, typically, an important issue in the care of this group. The HLPR Chair is currently being extended to be an autonomous mobility device to assist cognition by route and trajectory planning. This paper describes the design of HLPR Chair, its control architecture, and algorithms for autonomous planning and control using its unique kinematics.
Rapid Simple and Nested Polymerase Chain Reaction for the Diagnosis of Pneumocystis carinii pneumonia (1995)

We have developed a rapid and easy extraction procedure for polymerase chain reaction (PCR) protocols. Using this simplified step, we evaluated the sensitivity and the specificity of a simple PCR using the primers of Wakefield et al, and of a nested PCR, using new internal primers selected by us, in a total of 89 bronochoalveolar lavage (BAL) fluid samples from 43 immunosuppressed patients. In 13 patients, Pneumocystis carinii pneumonia (PCP) was diagnosed by immunofluorescent antibody (IFA) staining performed on BAL cells cytospun on microscope slides. In seven of these patients we attempted to estimate the post-treatment persistence of P. carinii in BAL, by PCR. After a rapid 2-h extraction procedure, simple and nested PCR were positive in all cases of PCP. SImple and nested PCR both had a 100% sensitivity and a 98 and 84% specificity respectively, compared to IFA. After completion of treatment, BAL liquids from asymptomatic patients were no longer positive by both PCR techniques, whereas the BAL fluid of a patient who was still symptomatic was positive by simple and nested PCR. In follow-up BAL fluids of patients with proven PCP, persistence of P. carinii was detected for a longer period by nested PCR than by simple PCR. Simple PCR is a very rapid and sensitive assay for the diagnosis of PCP in BAL fluid and gives clear-cut results in the case of doubtful IFA staining results. Nested PCR seems to improve the sensitivity of the detection of P. carinii in BAL fluid, but the clinical relevance of a positive result remains to be investigated..
A restoration methodology based on pre-planned source routing in ATM networks (2002)

This paper describes a restoration methodology for ATM networks that is built on top of a pre-planned source routing system in the network and operates on a connection-by-connection basis. Unlike most of the existing proposals that use dedicated spare facilities to protect network traffic against network fault conditions, this scheme simply takes advantage of unused network capacity to redirect traffic from the problem area. This restoration methodology requires the upstream neighboring switches to send messages back to the corresponding sources of the affected connections to find an alternative route. Given that this capability is being incorporated for new call setup in the signalling protocol for private ATM networks by the ATM Forum, the additional development cost and operations overhead associated with this restoration methodology is relatively insignificant. The performance of the proposed restoration methodology is discussed based on simulation studies for video on demand (VoD) applications.
Leveraging Semantic Web Techniques to Gain Situational Awareness (2007)

An invited talk at the Cyber Situational Awareness Workshop, Nov14-15, 2007 in Fairfax, VA.
The neural representation of visually evoked emotion is high-dimensional, categorical, and distributed across transmodal brain regions (2019)

Summary Central to our subjective lives is the experience of different emotions. Recent behavioral work mapping emotional responses to 2185 videos found that people experience upwards of 27 distinct emotions occupying a high-dimensional space, and that emotion categories, more so than affective dimensions (e.g., valence), organize self-reports of subjective experience. Here, we sought to identify the neural substrates of this high-dimensional space of emotional experience using fMRI responses to all 2185 videos. Our analyses demonstrated that (1) dozens of video-evoked emotions were accurately predicted from fMRI patterns in multiple brain regions with different regional configurations for individual emotions, (2) emotion categories better predicted cortical and subcortical responses than affective dimensions, outperforming visual and semantic covariates in transmodal regions, and (3) emotion-related fMRI responses had a cluster-like organization efficiently characterized by distinct categories. These results support an emerging theory of the high-dimensional emotion space, illuminating its neural foundations distributed across transmodal regions.
The multicast dissemination protocol (MDP) toolkit (2003)

The multicast dissemination protocol (MDP) provides reliable multicast file and data delivery on top of the generic UDP/IP multicast transport. Early work on MDP was deployed across the global Internet multicast backbone (Mbone) as part of the publicly available image multicaster (IMM) application. This paper describes more recent work on MDP resulting in the MDP version 2 (MDPv2) toolkit. This effort has significantly modified and generalized the MDP protocol and the associated software interface. Enhancements made to the protocol are suitable for a wide range of network environments. Additionally, integrated erasure-based repairing improves reliable multicast efficiency and robustness. We briefly discuss the protocol design, general performance characteristics, and ongoing MDP work including: initial rate-based congestion control design and results, network simulation and modeling, and asymmetric satellite operation.
Quality Parameters of Six Cultivars of Blueberry Using Computer Vision (2013)

Background. Blueberries are considered an important source of health benefits. This work studied six blueberry cultivars: "Duke," "Brigitta", "Elliott", "Centurion", "Star," and "Jewel", measuring quality parameters such as °Brix, pH, moisture content using standard techniques and shape, color, and fungal presence obtained by computer vision. The storage conditions were time (0-21 days), temperature (4 and 15°C), and relative humidity (75 and 90%). Results. Significant differences (P < 0.05) were detected between fresh cultivars in pH, °Brix, shape, and color. However, the main parameters which changed depending on storage conditions, increasing at higher temperature, were color (from blue to red) and fungal presence (from 0 to 15%), both detected using computer vision, which is important to determine a shelf life of 14 days for all cultivars. Similar behavior during storage was obtained for all cultivars. Conclusion. Computer vision proved to be a reliable and simple method to objectively determine blueberry decay during storage that can be used as an alternative approach to currently used subjective measurements.
Discovery of Diagnostic Biomarkers for Legionnaires' Disease: Virulence Gene Expression Profiling of Legionella pneumophila serogroup 1 in A/J Mouse Model (2009)

Background: Legionella pneumophila is the causative agent of Legionnaires' disease, a severe form of pneumonia. After L. pneumophila is inhaled through contaminated aerosols, it is phagocytized by alveolar macrophages, multiplies in a specialized phagosome approximately 10 h postinfection, and event...
World cup 1966 england (1966)

A strategy was devised for identifying regions of the mouse genome that are transcriptionally active in a temporally and spatially restricted manner during development. The approach is based on the introduction into embryonic stem cells of two types of lacZ reporter constructs that can be activated by flanking mouse genomic sequences. Embryonic stem cells containing the lacZ constructs were used to produce chimaeric mice. Developmental regulation of lacZ expression occurred at a high frequency. Molecular cloning of the flanking endogenous genes and introduction of these potential insertional mutations into the mouse germ line should provide an efficient means of identifying and mutating novel genes important for the control of mammalian development.
Evaluation of a New Regularization Prior for 3-D PET Reconstruction Including PSF Modeling (2012)

<?Pub Dtl=""?> The response of a PET system can be described by its characteristic Point Spread Function (PSF) representing the spatial degradation of a point source due to physical effects and system design. If the PSF is accounted for in the reconstruction algorithm, better image quality and spatial resolution may be achieved. Unfortunately, a common behaviour of unregularized iterative reconstruction techniques is represented by the increase of noise as the iterations proceed, while—on the other hand—a high number of iterations is usually needed to recover a significant percentage of the signal and to reach convergence, especially when resolution modelling is used in the reconstruction to recover the degraded signal. Moreover, a recognized effect of PSF-based reconstructions is the overenhancement of sharp transitions (edges) in the reconstructed images.
Airborne LiDAR In-flight Accuracy Estimation (2009)

A flexible quality-monitoring tool that assesses data quality in-flight avoids costly problems that are currently detected only in post-processing.
Negative Group Velocities of Lamb-Type Waves in a Glass/Water/Glass Structure Controlled by the Thickness of Water Layer (2007)

In this paper, we described the characteristics of negative group velocities of Lamb-type waves in a glass/water/glass structure controlled by the thickness of the water layer. Lamb waves in an elastic plate have negative group velocities. It is known that the range of existence of negative group velocities depends on the Poisson's ratio of the elastic plate. However, in the case of Lamb waves, their negative group velocities depend on the physical parameters of each material; therefore, we fabricated a structure such that the negative group velocities of Lamb waves are controllable without the limitation of the parameters of each material. The waves in such a structure are referred to as the Lamb-type waves. Using numerical calculations, we determined that the negative group velocities of the Lamb-type waves can be varied by changing the geometric parameters, and the negative group velocities are changed although the frequencies are fixed. Additionally, we found that the Lamb-type waves have various characteristics corresponding to the propagation modes.
Event-Driven Semantic Concept Discovery by Exploiting Weakly Tagged Internet Images (2014)

Analysis and detection of complex events in videos require a semantic representation of the video content. Existing video semantic representation methods typically require users to pre-define an exhaustive concept lexicon and manually annotate the presence of the concepts in each video, which is infeasible for real-world video event detection problems. In this paper, we propose an automatic semantic concept discovery scheme by exploiting Internet images and their associated tags. Given a target event and its textual descriptions, we crawl a collection of images and their associated tags by performing text based image search using the noun and verb pairs extracted from the event textual descriptions. The system first identifies the candidate concepts for an event by measuring whether a tag is a meaningful word and visually detectable. Then a concept visual model is built for each candidate concept using a SVM classifier with probabilistic output. Finally, the concept models are applied to generate concept based video representations. We use the TRECVID Multimedia Event Detection (MED) 2013 as our video test set and crawl 400K Flickr images to automatically discover 2, 000 visual concepts. We show significant performance gains of the proposed concept discovery method over different video event detection tasks including supervised event modeling over concept space and semantic based zero-shot retrieval without training examples. Importantly, we show the proposed method of automatic concept discovery outperforms other well-known concept library construction approaches such as Classemes and ImageNet by a large margin (228%) in zero-shot event retrieval. Finally, subjective evaluation by humans also confirms clear superiority of the proposed method in discovering concepts for event representation.
Xyloglucan as a Novel Vehicle for Timolol: Pharmacokinetics and Pressure Lowering Activity in Rabbits (2000)

This study was aimed at verifying the performances of a mucoadhesive polysaccharide from tamarind seed (xyloglucan or TSP, tamarind seed polysaccharide) as an adjuvant for ophthalmic vehicles containing timolol. Three formulations (one experimental vehicle based on TSP and two reference commercial eye drops) containing 5 mg/ml timolol base equivalents were administered to the eyes of pigmented rabbits. Drug concentrations in tear fluid, cornea, iris-ciliary body, aqueous humor and plasma were determined, as well as intraocular pressure. The polymer under investigation, in spite of a comparatively low viscosity, produced high timolol concentrations in the ocular tissues and a low systemic absorption. The performances of the TSP vehicle were comparable to those of a reference "in situ" gelling formulation (Timoptic XE). The results point to TSP as a potentially useful adjuvant for ophthalmic delivery systems.
The 'Heiner Affair': A Matter of Professional Concern (2010)

A statement expressing concern over the unresolved Heiner Affair has been signed by the Australian Society of Archivists Inc and the Records Management Association of Australasia, together with academics, legal practitioners and former judges. Their aim is to seek from this inquiry the establishment of an independent State Archivist, that report directly to Parliament on matters relating to the creation and disposal of public records.
An efficient GPU out‐of‐core framework for interactive rendering of large‐scale CAD models (2016)

Abstract Real‐time rendering of large‐scale engineering computer‐aided design (CAD) models has been recognized as a challenging task. Because of the constraints of limited graphics processing unit (GPU) memory size and computation capacity, a massive model with hundreds of millions of triangles cannot be loaded and rendered in real‐time using most of modern GPUs. In this paper, an efficient GPU out‐of‐core framework is proposed for interactively visualizing large‐scale CAD models. To improve efficiency of data fetching from CPU host memory to GPU device memory, a parallel offline geometry compression scheme is introduced to minimize the storage cost of each primitive by compressing the levels of detail (LOD) geometries into a highly compact format. At the rendering stage, occlusion culling and LOD processing algorithms are integrated and implemented with an efficient GPU‐based approach to determine a minimal scale of primitives to be transferred for each frame. A prototype software system is developed to preprocess and render massive CAD models with the proposed framework. Experimental results show that users can walkthrough massive CAD models with hundreds of millions of triangles at high frame rates using our framework. Copyright © 2016 John Wiley & Sons, Ltd.
Carbapenem-Resistant Isolates of <i>Klebsiella pneumoniae</i> in China and Detection of a Conjugative Plasmid ( <i>bla</i> <sub>KPC-2</sub> plus <i>qnrB4</i> ) and a <i>bla</i> <sub>IMP-4</sub> Gene (2007)

Wei et al. ([13][1]) recently described the emergence of plasmid-mediated KPC-2 carbapenemase in a Klebsiella pneumoniae isolate from China. Interestingly, KPC-producing isolates, which were initially restricted to hospitals located in the New York City area ([2][2]), have recently been detected in
Circadian periodicity of cerebral blood flow revealed by laser-Doppler flowmetry in awake rats: relation to blood pressure and activity (2005)

Cardiovascular parameters such as arterial blood pressure (ABP) and heart rate display pronounced circadian variation. The present study was performed to detect whether there is a circadian periodicity in the regulation of cerebral perfusion. Normotensive Sprague-Dawley rats (SDR, approximately 15 wk old) and hypertensive (mREN2)27 transgenic rats (TGR, approximately 12 wk old) were instrumented in the abdominal aorta with a blood pressure sensor coupled to a telemetry system for continuous recording of ABP, heart rate, and locomotor activity. After 5-12 days, a laser-Doppler flow (LDF) probe was attached to the skull by means of a guiding device to measure changes in brain cortical blood flow (CBF). After the animals recovered from anesthesia, measurements were taken for 3-4 days. The time series were analyzed with respect to the midline estimating statistic of rhythm (i.e., mean value of a periodic event after fit to a cosine function), amplitude, and acrophase (i.e., phase angle that corresponds to the peak of a given period) of the 24-h period. The LDF signal displayed a significant circadian rhythm, with the peak occurring at around midnight in SDR and TGR, despite inverse periodicity of ABP in TGR. This finding suggests independence of LDF periodicity from ABP regulation. Furthermore, the acrophase of the LDF was consistently found before the acrophase of the activity. From the present data, it is concluded that there is a circadian periodicity in the regulation of cerebral perfusion that is independent of circadian changes in ABP and probably is also independent of locomotor activity. The presence of a circadian periodicity in CBF may have implications for the occurrence of diurnal alterations in cerebrovascular events in humans.
Macrophage migration inhibitory factor expression is increased in pituitary adenoma cell nuclei (2003)

Macrophage migration inhibitory factor (MIF) is an essential regulator of the macrophage responses to endotoxin. MIF also has the ability to override the anti-inflammatory actions of glucocorticoids during an immune response, and is thus an important pro-inflammatory factor. The presence of MIF in cells of the anterior pituitary has been described, and high levels of MIF in other rapidly proliferating tIssues have also been demonstrated. It has been hypothesised that MIF release from these cells is influenced by the hypothalamo-pituitary-adrenal axis, and that ACTH and MIF are released simultaneously to exert counter-regulatory effects on cortisol. However, another intracellular role for MIF has also been suggested as it has been shown that MIF exerts an effect on the inhibitory cell cycle control protein p27 through an interaction with Jab1, a protein implicated in p27 degradation. We studied MIF expression in different normal and adenomatous human pituitary samples using immunohistochemistry and RT-PCR. There was evidence of co-immunoprecipitation of MIF with Jab1, suggesting an interaction of the two proteins. Our results showed that there is increased expression of MIF protein in the nuclei of all pituitary adenomas compared with normal tIssue (P=0.0067), but there was no statistically significant difference in nuclear MIF expression between the different adenoma types. Nuclear MIF expression correlated positively with p27 and its phosphorylated form in normal tIssue (P=0.0028 and P<0.0001); however, this relationship was not seen in the adenoma samples. Cytoplasmic expression of MIF was found to be variable both in normal and adenomatous samples, with no consistent pattern. MIF mRNA was demonstrated to be present in all tumour and normal samples studied. Somatotroph tumours showed higher MIF mRNA expression compared with normal pituitary or other types of adenomas. In conclusion, MIF is expressed in cell nuclei in pituitary adenomas to a greater extent than in normal pituitary tIssue. We speculate that it may play a role in the control of the cell cycle, but whether its higher level in adenomas is a cause or a consequence of the tumorigenic process remains to be clarified.
Characterization and transcriptional analysis of the gene cluster for coronafacic acid, the polyketide component of the phytotoxin coronatine (1995)

Coronafacic acid (CFA), the polyketide component of the phytotoxin coronatine (COR), is activated and coupled to coronamic acid via amide bond formation, a biosynthetic step presumably catalyzed by the CFA ligase (cfl) gene product. The COR biosynthetic gene cluster in Pseudomonas syringae pv. glycinea PG4180 is located within a 32-kb region of a 90-kb plasmid designated p4180A. In the present study, a cloned region of p4180A complemented all CFA- mutants spanning an 18.8-kb region of the COR biosynthetic cluster. The genetic evidence presented in this study indicates that cfl and the CFA biosynthetic gene cluster are encoded by a single transcript and that transcription of all of the genes in this operon is directed by the cfl promoter. The cfl promoter was localized to a 0.37-kb region upstream of the transcriptional start site by progressive subcloning in pRG960sd, a vector containing a promoterless glucuronidase gene. Transcription of the cfl/CFA operon was temperature sensitive and showed maximal glucuronidase activity at 18 degrees C. Furthermore, transcription of the cfl/CFA operon was dependent on the functional activity of a modified two-component regulatory system located within the COR biosynthetic gene cluster. Thermoregulation of the cfl/CFA operon and the coronamic acid biosynthetic gene cluster via the modified two-component regulatory system is discussed.