gebhart/patrepeval huggingface dataset:
Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli (1989)

The sequence of 4,362 nucleotides encompassing the proU operon of Escherichia coli was determined. Three open reading frames were identified whose orientation, order, location, and sizes were in close accord with genetic evidence for three cistrons (proV, proW, and proX) in this operon. Similarities in primary structure were observed between (i) the deduced sequence of ProV with membrane-associated components of other binding-protein-dependent transport systems, in the nucleotide-binding region of each of the latter proteins, and (ii) that of ProW with integral membrane components of the transport systems above. The DNA sequence data also conclusively established that ProX represents the periplasmic glycine betaine-binding protein. Two copies of repetitive extragenic palindromic sequences were identified beyond the 3' end of the proX gene. The primer extension technique was used to identify the 5' ends of proU mRNA species that are present in cells grown at high osmolarity; the results suggest that at least some of the osmotically induced proU transcripts have a long leader region, extending as much as 250 base pairs upstream of the proV gene. Evidence was also obtained for the existence of a sequence-directed bend in DNA in the upstream regulatory region of the proU operon.
Identification of novel Prominin-1/CD133 splice variants with alternative C-termini and their expression in epididymis and testis (2004)

Prominin-1/CD133 is a five-membrane-span glycoprotein that is thought to act as an organizer of plasma-membrane protrusions. Here, we report the molecular and cell-biological characterization of four novel prominin-1 splice variants isolated from a mouse testis cDNA library and referred to as prominin-1.s3 to prominin-1.s6. Compared with kidney-derived prominin-1.s1, the s3, s4 and s5 variants contain a distinct cytoplasmic C-terminal domain. The s4 and s5 variants bear, in addition, two and one inframe deletion(s), respectively, in the extracellular domains. The s6 variant displays a truncated C-terminal domain caused by a premature termination resulting from intron retention. Upon their ectopic expression in Chinese hamster ovary cells, the s3 and s6 variants were found to be concentrated in plasma-membrane protrusions, whereas the s4 and s5 variants did not reach the cell surface. Biochemical analyses suggest that most of the prominin-1 in the adult male reproductive system is expressed as the s6 variant. Immunohistological and electron microscopic analyses show that prominin-1 is: (1) confined to the apical surface of the epithelium all along the epididymal duct, with the exception of the initial segment; (2) concentrated in stereocilia of the epididymal duct epithelium; and (3) found on the tail of developing spermatozoa in seminiferous tubules. Our data suggest that prominin-1 is involved in the formation and/or stabilization of epididymal stereocilia and the tail of spermatozoa, and hence might play a dual role in the biogenesis of spermatozoa.
Tracking a moving target in cluttered environments with ranging radios (2008)

In this paper, we propose a framework for utilizing fixed, ultra-wideband ranging radio nodes to track a moving target node through walls in a cluttered environment. We examine both the case where the locations of the fixed nodes are known as well as the case where they are unknown. For the case when the fixed node locations are known, we derive a Bayesian room-level tracking method that takes advantage of the structural characteristics of the environment to ensure robustness to noise. We also develop a method using mixtures of Gaussians to model the noise characteristics of the radios. For the case of unknown fixed node locations, we present a two-step approach that first reconstructs the target node's path and then uses that path to determine the locations of the fixed nodes. We reconstruct the path by projecting down from a higher-dimensional measurement space to the 2D environment space using non-linear dimensionality reduction with Gaussian Process Latent Variable Models(GPLVMs). We then utilize the reconstructed path to map the locations of the fixed nodes using a Bayesian occupancy grid. We present experimental results verifying our methods in an office environment. Our methods are successful at tracking a moving target node and mapping the locations of fixed nodes using radio ranging data that are both noisy and intermittent.
Cellular mechanisms of netrin function: Long-range and short-range actions (2000)

Netrins are secreted proteins that direct axon extension and cell migration during neural development. They are bifunctional cues that act as an attractant for some cell types and as a repellent for others. Several lines of evidence suggest that two classes of receptors, the deleted in colorectal cancer (DCC) family and the UNC-5 family, mediate the attractant and repellent response to netrin. Although netrins were first identified as diffusible long-range cues for developing axons, recent findings provide evidence that they also function as short-range cues close to the surface of the cells that produce them. This short-range function of netrin contributes to guiding neurite outgrowth and mediating cell-cell interactions during development and perhaps also in adults.
Angiogenesis Induced by "Normal" Human Breast Tissue: A Probable Marker for Precancer (1982)

Normal human breast lobules, freshly isolated by precision microdissection of tissue stained with methylene blue chloride, were assayed for their ability to induce neovascularization (angiogenesis) in rabbit irises. Histologically, normal lobules from cancerous breast induced angiogenesis twice as often as lobules from noncancerous breasts, suggesting that preneoplastic transformation is diffuse.
An Electrochemical Theory for Oxygen Reboil (1966)

Oxygen reboil often occurs when glass is melted in air in platinum containers. Oxygen blisters develop even when well‐fined glass is remelted under similar conditions. Experiments show that this reboil is prevented or greatly reduced by: (1) Eliminating electron conductors from the system, (2) removing oxygen from the atmosphere over the melt, (3) applying an external bucking potential, (4) reversing concentration gradients, or (5) removing temperature gradients. These results suggest that oxygen reboil, which occurs at a platinum‐glass interface, results from the discharge of a concentration cell or a thermal cell. The proposed mechanism is: O 2− →½ O 2 + 2e − at the anode and ½ O 2 + 2e − → O 2− at the cathode, with electron transport in the platinum and, to complete the circuit, alkali ions in the glass.
Nonsmooth H<sub>∞</sub>Synthesis (2006)

We develop nonsmooth optimization techniques to solve H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> synthesis problems under additional structural constraints on the controller. Our approach avoids the use of Lyapunov variables and therefore leads to moderate size optimization programs even for very large systems. The proposed framework is versatile and can accommodate a number of challenging design problems including static, fixed-order, fixed-structure, decentralized control, design of PID controllers and simultaneous design and stabilization problems. Our algorithmic strategy uses generalized gradients and bundling techniques suited for the H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">∞</sub> norm and other nonsmooth performance criteria. We compute descent directions by solving quadratic programs and generate steps via line search. Convergence to a critical point from an arbitrary starting point is proved and numerical tests are included to validate our methods. The proposed approach proves to be efficient even for systems with several hundreds of states.
Programmable Remote Traffic Monitoring Method Using Active Network Approach (2001)

As the Internet has become an infrastructure for the global communication, a network failure and a quality degradation have become a serious problem. In order to solve the problem, a network monitoring system which monitors the traffic of Internet in real time is strongly desired. Traffic monitors which collect the statistics from captured packets play a key roll in the system; however, they are not flexible enough for being used in the rapidly changing Internet. The traditional approach such that a new traffic monitor is developed for a new requirement results in a long turn around time of the development. Therefore, we have proposed a flexible network monitoring system which consists of programmable traffic monitors. Traffic monitors are made programmable by introducing active network techniques; therefore, we call the network monitoring system as the active monitor network. This paper describes the implementation and evaluation of the active monitor network.
Reactions of 2-acyloxyisobutyryl halides with nucleosides. II. Reactions of adenosine (1973)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReactions of 2-acyloxyisobutyryl halides with nucleosides. II. Reactions of adenosineA. F. Russell, S. Greenberg, and J. G. MoffattCite this: J. Am. Chem. Soc. 1973, 95, 12, 4025–4030Publication Date (Print):June 1, 1973Publication History Published online1 May 2002Published inissue 1 June 1973https://pubs.acs.org/doi/10.1021/ja00793a032https://doi.org/10.1021/ja00793a032research-articleACS PublicationsRequest reuse permissionsArticle Views621Altmetric-Citations75LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Analysis of the expression of NB1 antigen using two monoclonal antibodies (1996)

NB1 specificity is made up of at least two separate epitopes. The expression of NB1 varied among antigen-positive individuals. While NB1 is expressed by a 56- to 64-kDa glycoprotein, the structure of this protein on antigen-negative cells has not been determined.
Survival from alcoholic hepatitis has not improved over time (2018)

There has been no improvement in mortality from AH. This is not explained by changes in severity of disease. This emphasises the urgent need for effective treatments for this alcoholic hepatitis.
Effect of vulcanizing system on the crosslink density of nitrile rubber compounds (2005)

The synergistic activity of binary accelerator systems in rubber vulcanization is well known. Binary accelerator systems are being widely used in industry and are becoming increasingly popular because of the fact that such mixed systems can produce a vulcanizate with superior mechanical properties compared to those of stock cured with a single accelerator. The authors have studied the performance of a binary accelerator system based on cyclohexyl benzothiazole sulfenamide (CBS), tetramethyl thiuram disulphide (TMTD) in the sulfur vulcanization of nitrile rubber. The amount of sulfur and accelerator was varied to change the network crosslink density of vulcanizates. The observed mutual activity has been discussed based on the mechanical properties and crosslink density. The physical crosslink density of the various nitrile rubber mixes was estimated using the Kinetic Theory of Elasticity. The mechanical properties of the various rubber compounds were related to the corresponding crosslink density estimated for each compound. © 2005 Wiley Periodicals, Inc. J Appl Polym Sci 96: 2440–2445, 2005
Group Contribution Model for Predicting Viscosity of Fatty Compounds (2007)

In the present work, a group contribution model is proposed for estimating the dynamic viscosity of fatty compounds. For the major components involved in the vegetable oil industry (such as fatty acids, esters and alcohols, triacylglycerols, and partial acylglycerols), the optimized parameters are reported. In order to improve the data bank gathered from the literature, viscosity data were measured as a function of temperature for three saturated and three unsaturated fatty esters, one unsaturated fatty acid, and one unsaturated triacylglycerol. These last two are unavailable data in the literature (linolenic acid and trilinolenin). A simple method of calculation is also proposed to predict the dynamic viscosity of vegetable oils as an extended applicability of our equation. The model can be a valuable tool for designing processes and equipment for the oil industry, including edible and non-edible compounds, such as biodiesel.
Front-View Vehicle Damage Detection using Roadway Surveillance Camera Images (2019)

Vehicle body damage detection from still images has received considerable interest in the computer vision community in recent years. Existing methods are typically developed towards the auto insurance industry to minimize the claim leakage problem. Earlier studies utilized images taken from short proximity (< 3 meters) to the vehicle or to the damaged region of vehicle. In this study, we investigate the vehicle frontal body damage detection using roadway surveillance camera images. The proposed method utilizes deep learning based object detection and image classification methods to determine damage status of a vehicle. The proposed method combines the symmetry property of vehicles’ frontal view and transfer learning concept in its inference process. Experimental results show that the proposed method achieves 91 % accuracy on a test dataset.
DYNAMIC SHORT-TERM TRAFFIC FLOW FORECASTING FOR CONGESTED FREEWAY (2006)

A new approach is proposed by integrating time series model with nonparametric approach to predict short-term congested freeway traffic flows. The key difference between the paper and the previous researches is that the proposed model can dynamically adapt to different traffic scenarios with a new introduced variable z(sub t) to represent the interactions of exogenous unquantifiable or non-easily quantifiable impact factors to the traffic flow pattern. Two variants, a single-step prediction model and multi-step model of the proposed approach are studied and compared. The application results of these two models to a real world traffic network showed that the multi-step model is better than the single-step model. Furthermore, the built-in B+ tree structure of this approach improves the computation and searching speed, making the future real time application possible. For the covering abstract see ITRD E134653.
New Carrier Method for Propellants Offers Flexibility, Simplicity, and Performance (2008)

Abstract Propellant-based well stimulation is an accepted technology in the oil industry; a number of papers have been published regarding its advantages and advancements (Folse et al. 2001; Gilliat et al. 1999; Yang et al. 1992). The additions of propellant stimulation modeling software and high-speed pressure recorders have greatly assisted in the development and maturing of the technology (Schatz et al. 1999). The most commonly used propellant for near wellbore stimulation is the propellant sleeve. Propellant sleeve is simply a hollow tube of propellant that is positioned outside of a standard perforating gun and held into place by retaining rings. The detonation of the perforating charges ignites the propellant. The internal ballistic train of the perforating gun is not affected by the propellant because of the sleeve's external placement. This system is both reliable and effective. A second and less frequently used technique places the propellant inside a vented hollow steel carrier, allowing propellant stimulation without the need of introducing new perforations. Unlike the propellant sleeve system, this system uses detonating cord to ignite the propellant. This method has the ability to stimulate virtually an unlimited amount of borehole by simply interconnecting the vented propellant carriers. However, the ballistic train is exposed to wellbore fluids and pressures. Therefore, when deploying longer intervals (greater than 7 m), the propellant system must be able to reliably continue the ballistics train between interconnected propellant carriers. In the past, the wet-connect system used was unreliable and limited the system to low wellbore pressure applications. Consequently, this method has struggled to gain acceptance primarily due to a lack of a reliable ignition system combined with limited stimulation pressure data. This paper discusses a new propellant stimulation system that addresses reliability issues that have plagued propellant stimulation systems in the past. It also offers case studies that demonstrate the effectiveness of the new system, performance of the optimized propellant formula as well as the value created from the system's performance and flexibility.
Improvement in Usual Gait Speed Predicts Better Survival in Older Adults (2007)

Improvement in usual gait speed predicts a substantial reduction in mortality. Because gait speed is easily measured, clinically interpretable, and potentially modifiable, it may be a useful "vital sign" for older adults. Further research is needed to determine whether interventions to improve gait speed affect survival.
[Experimental evidence of an antihypertensive and hypocholesterolemic effect of oil of argan, Argania sideroxylon]. (2000)

The chronic ingestion of 5 ml/kg/d of Argan oil by spontaneously hypertensive rats restores normal blood pressure and induces hypocholesterolaemia. In order to confirm these results Meriones shawi, a rodent of the Gerbillideae family, was tested as a second animal model. Meriones submitted to a hypercaloric diet and physical inactivity became fat, and exhibited hypertension, dyslipidaemia and hyperinsulinaemia. When treated for two months with the same dosage regimen, decreases in glycaemia, total plasmatic cholesterol, LDL, insulinaemia and systolic and diastolic blood pressures of 4.4 per cent, 14.4 per cent, 32.5 per cent, 26.8 per cent, 28.8 per cent and 30.5 per cent were simultaneously observed. Increases in LDH and of TG of 27.9 per cent and 16.2 per cent respectively were also observed. No effect on body weight occurred. The action of polyunsatured fatty acids of the Argan oil is predominant, but in addition, other constituents play an active part.
The Evolution of Urology in Dallas: A Historical Perspective (1990)

Important events in Texas surgical history are mostly unrecognized, such as the first thoracic surgery performed by a member of the Cabeza de Vaca expedition in 1535 (Thompson JE: Sagittectomy, first recorded surgical procedure in the American Southwest, 1535: the journey and ministrations of Alvar Nuñez Cabeza de Vaca, N Engl J Med 289: 1403–1407, 1973). Dallas urological surgery has developed to world-class recognition since I came to the University of Texas Southwestern Medical School as a student and Dr. A. I. Folsom was the Chief of Urology. Accordingly, I asked the most knowledgeable person available to do a short viewpoint review of urologic surgery growth and development in Dallas. This was ably done by Dr. Harry Spence who, in addition to his role in urology, served as President of the Medical Staff of Baylor University Medical Center in 1966.
Imágenes-tipo y "modus orandi": las variantes iconográficas del santo en la pintura del Renacimiento español (1989)

Almost all transfection protocols for mammalian cells use a drug resistance gene for the selection of transfected cells. However, it always requires the characterization of each isolated clone regarding transgene expression, which is time-consuming and labor-intensive. In the current study, we developed a novel method to selectively isolate clones with high transgene expression without drug selection. Porcine embryonic fibroblasts were transfected with pCEIEnd, an expression vector that simultaneously expresses enhanced green fluorescent protein (EGFP) and endo-b-galactosidase C(EndoGalC; an enzyme capable of digesting cell surface a-Gal epitope) upon transfection. After transfection, the surviving cells were briefly treated with IB4SAP (a-Gal epitope-specific BS-I-B4 lectin conjugated with a toxin saporin). The treated cells were then allowed to grow in normal medium, during which only cells strongly expressing EndoGalC and EGFP would survive because of the absence of a-Gal epitopes on their cell surface. Almost all the surviving colonies after IB4SAP treatment were in fact negative for BS-I-B4 staining, and also strongly expressed EGFP. This system would be particularly valuable for researchers who wish to perform large-scale production of therapeutically important recombinant proteins.
AIR-2: An Aurora/Ipl1-related Protein Kinase Associated with Chromosomes and Midbody Microtubules Is Required for Polar Body Extrusion and Cytokinesis in <i>Caenorhabditis elegans</i> Embryos (1998)

An emerging family of kinases related to the Drosophila Aurora and budding yeast Ipl1 proteins has been implicated in chromosome segregation and mitotic spindle formation in a number of organisms. Unlike other Aurora/Ipl1-related kinases, the Caenorhabditis elegans orthologue, AIR-2, is associated with meiotic and mitotic chromosomes. AIR-2 is initially localized to the chromosomes of the most mature prophase I-arrested oocyte residing next to the spermatheca. This localization is dependent on the presence of sperm in the spermatheca. After fertilization, AIR-2 remains associated with chromosomes during each meiotic division. However, during both meiotic anaphases, AIR-2 is present between the separating chromosomes. AIR-2 also remains associated with both extruded polar bodies. In the embryo, AIR-2 is found on metaphase chromosomes, moves to midbody microtubules at anaphase, and then persists at the cytokinesis remnant. Disruption of AIR-2 expression by RNA- mediated interference produces entire broods of one-cell embryos that have executed multiple cell cycles in the complete absence of cytokinesis. The embryos accumulate large amounts of DNA and microtubule asters. Polar bodies are not extruded, but remain in the embryo where they continue to replicate. The cytokinesis defect appears to be late in the cell cycle because transient cleavage furrows initiate at the proper location, but regress before the division is complete. Additionally, staining with a marker of midbody microtubules revealed that at least some of the components of the midbody are not well localized in the absence of AIR-2 activity. Our results suggest that during each meiotic and mitotic division, AIR-2 may coordinate the congression of metaphase chromosomes with the subsequent events of polar body extrusion and cytokinesis.