gebhart/patrepeval huggingface dataset:
Bourgueticrinus utriculatus (Valette) from the Senonian ofthe Cracow Upland (1972)

In the paper a description is given of a calyx of the so far unknown in Poland crinoid Bourgueticrinus utriculatus (Valette) collected in the Lower Campanian marls of the Cracow Upland.
Abdominal Pain After Roux-En-Y Gastric Bypass for Morbid Obesity (2018)

Abdominal pain should be part of follow-up consultations post Roux-en-Y gastric bypass. Future studies should focus on combined evaluations before and after surgery to enlighten potential casual relationships between abdominal pain and Roux-en-Y gastric bypass.
Purification of Ribonucleic Acid Polymerase from SP82-infected Bacillus subtilis (1974)

A procedure for the isolation of RNA polymerase fromBacillus subtilis is presented which yields enzyme with very high specific activity.RNA polymerases isolated from uninfected and from SP82-infected B. subtilis
Extensive Fungal Diversity in Plant Roots (2002)

Fungi play crucially important roles in the biosphere, mediating many ecological processes. Despite this, the fungal diversity in natural habitats is poorly known. Using newly designed fungal-specific polymerase chain reaction (PCR) primers ([1][1]), we examined total fungal diversity in a
Antiviral Activity of <i>N</i> -Phenyl- <i>N</i> ′-Aryl- or Alkylthiourea Derivatives in Coxsackie Virus Infections in Mice (1974)

The effect of five derivatives of N-phenyl-N'-aryl- or alkylthiourea-inhibitors of the multiplication of Coxsackie virus B1 and other picornaviruses in vitro-was tested in experimental infections with Coxsackie viruses B1, B3, A6, and A7 in newborn mice. Under the action of N-phenyl-N'-3-hydroxyphenylthiourea (no. 23) and N-phenyl-N'-4-carboxy-5-hydroxyphenylthiourea (no. 20) a two- to threefold reduction in mortality was observed, as well as an appreciable delay in the course of the disease (mean effective dose, lengthening by 2 to 6 days) after infection with Coxsackie viruses B1, B3, and A7. The infection with Coxsackie virus A6 was affected only by compound no. 23 and, at that, to a low degree. If the antiviral effect is to be obtained, the compounds must be applied daily (once subcutaneously) from the 24th to the 144th h after virus inoculation, a period which corresponds to the incubation period and the beginning of the manifested infection. On the basis of these data, as well as of the relatively high selectivity (therapeutic index of 3 to 20), the two indicated substances may be considered to be reliable antiviral chemotherapeutic agents.
Effect of protein kinase Cgamma on gap junction disassembly in lens epithelial cells and retinal cells in culture. (2002)

PKCgamma can be co-immunoprecipitated with Cx43 from lens epithelial cells using phorbol ester activation. PKCgamma phosphorylates Cx43 on serine and this causes disassembly and loss of gap junction Cx43 from the cell surface. Overexpression of PKCgamma confirmed that only this PKC isoform caused the loss of cell surface Cx43. Overexpression of PKCalpha, the other major lens PKC isoform, caused an increase in cell surface Cx43. The presence of PKCgamma and loss of surface Cx43 from two retinal cell lines, WERI and Y79, upon phorbol ester activation further suggests that activation of PKCgamma may be a common mechanism for control of cell surface Cx43.
Microfluidic blood plasma separation via bulk electrohydrodynamic flows (2006)

An effective mechanism for rapid and efficient microfluidic particle trapping and concentration is proposed without requiring any mechanically moving parts. When a voltage beyond the threshold atmospheric ionization value is applied on a sharp electrode tip mounted at an angle above a microfluidic liquid chamber, the bulk electrohydrodynamic air thrust that is generated results in interfacial shear and, hence, primary azimuthal liquid surface recirculation. This discharge driven vortex mechanism, in turn, causes a secondary bulk meridional liquid recirculation, which produces an inward radial force near the bottom of the chamber. Particles suspended in the liquid are then rapidly convected by the bulk recirculation toward the bottom, where the inward radial force causes them to spiral in a helical swirl-like fashion toward a stagnation point. In particular, we show that these flows, similar to Batchelor flows occurring in a cylindrical liquid column between a stationary and rotating disk, can be used for the separation of red blood cells from blood plasma in a miniaturized device.
DIPLOID SPORE FORMATION AND OTHER MEIOTIC EFFECTS OF TWO CELL-DIVISION-CYCLE MUTATIONS OF <i>SACCHAROMYCES CEREVISIAE</i> (1980)

The meiotic effects of two cell-division-cycle mutations of Saccharomyces cerevisiae (cdc5 and cdc14) have been examined. These mutations were isolated by L.H. HARTWELL and his colleagues and characterized as defective in mitosis, causing a temperature-sensitive arrest in late nuclear division. When subjected to the restrictive temperature in meiosis, diploid cells homozygous for either of these mutations generally proceeded through premeiotic DNA synthesis and commitment to meiotic levels of recombination, but then arrested at a stage following spindle pole body (SPB) duplication and separation. The two SPBs lacked the interconnection by spindle microtubules typical of the complete meiosis I spindle. Challenge of these homozygotes by a semi-restrictive temperature often caused the production of asci containing two diploid spores. Genetic analysis of the viable pairs of spores revealed that each spore had become homozygous for centromere-linked markers significantly more frequently than for distal markers, indicating that the two spores each contained pairs of sister centromeres that had co-segregated in the reductional division of meiosis I. Ultrastructural analysis of the cdc5 homozygote demonstrated that these cells had completed meiosis I and formed two meiosis II spindles, but that the latter remained unusually short. This resulted in the encapsulation of both poles of each spindle within a single spore wall. These mutations therefore are defective in both meiotic divisions, as well as in the mitotic division described originally.
ATUAÇÃO DO ENFERMEIRO NO TRATAMENTO DE RECÉM-NASCIDO PORTADOR DE DEISCÊNCIA DE SUTURA EM FERIDA CIRÚRGICA PARA CORREÇÃO DE MIELOMENINGOCELE (2005)

The aim of this study was to describe the treatment and evolution of a newborn's myelomeningocele closure wound. The neonate was admitted on the 18th postoperative day, when poor perfusion, dermis' total compromising, necrosis, unstuck edges, muscle exposure and exudation at the suture line were observed. Calcium alginate, essential fatty acids and papain were used. Total scarring was obtained on the 46th day of treatment.
Pupil size reveals preparatory processes in the generation of pro‐saccades and anti‐saccades (2015)

The ability to generate flexible behaviors to accommodate changing goals in response to identical sensory stimuli is a signature that is inherited in humans and higher-level animals. In the oculomotor system, this function has often been examined with the anti-saccade task, in which subjects are instructed, prior to stimulus appearance, to either automatically look at the peripheral stimulus (pro-saccade) or to suppress the automatic response and voluntarily look in the opposite direction from the stimulus (anti-saccade). Distinct neural preparatory activity between the pro-saccade and anti-saccade conditions has been well documented, particularly in the superior colliculus (SC) and the frontal eye field (FEF), and this has shown higher inhibition-related fixation activity in preparation for anti-saccades than in preparation for pro-saccades. Moreover, the level of preparatory activity related to motor preparation is negatively correlated with reaction times. We hypothesised that preparatory signals may be reflected in pupil size through a link between the SC and the pupil control circuitry. Here, we examined human pupil dynamics during saccade preparation prior to the execution of pro-saccades and anti-saccades. Pupil size was larger in preparation for correct anti-saccades than in preparation for correct pro-saccades and erroneous pro-saccades made in the anti-saccade condition. Furthermore, larger pupil dilation prior to stimulus appearance accompanied saccades with faster reaction times, with a trial-by-trial correlation between dilation size and anti-saccade reaction times. Overall, our results demonstrate that pupil size is modulated by saccade preparation, and neural activity in the SC, together with the FEF, supports these findings, providing unique insights into the neural substrate coordinating cognitive processing and pupil diameter.
Identification of a mutation in the Indian Hedgehog (<i>IHH</i>) gene causing brachydactyly type A1 and evidence for a third locus (2003)

Brachydactyly (BD) is a term used to describe inherited anomalies of the hands generally characterised by shortened phalanges or metacarpals. Initially, the brachydactylies were grouped into five different classes (A-E), with three subtypes of A.1 Later work revised and extended the classification of BD.2,3 In type A, shortening is primarily confined to the middle phalanges. Subtype A1 (BDA1, OMIM 112500) is distinguished by hypoplastic middle phalanges (especially the 2nd and 5th digits), with either distal or terminal symphalangism depending on the severity. In addition, shortening of the proximal phalanges of the thumb, the metacarpals, metatarsals, or the big toe are also observed in these patients. Short stature is often associated with BDA1 patients.

Despite being the first syndrome described with Mendelian autosomal dominant inheritance in 1903,4, the genetic aetiology of BDA was not reported until 2001.5 Suspecting that cell proliferation or differentiation factors could be the culprit causing BDA1, our group screened markers near several candidate genes in two families diagnosed with BDA1. No linkage was observed.6 We also chose to look for mutations of the PAX3 genes in these families by direct sequencing of all the exons, but no significant mutation was identified (unpublished data).

In 2000, a locus for BDA1 was mapped to 2q35-q36 in two unrelated Chinese families.7 Refined mapping and mutation screening of candidate genes in the region by the same group identified missense mutations in the Indian Hedgehog gene ( IHH) of the affected subjects in three unrelated families.5 The missense mutations, located at the amino terminus of the IHH protein, are conserved among the Hedgehog family proteins. Local and long range cell proliferation signalling functions were suggested to reside within the amino-terminus domain. Interestingly, ROR2 and CDMP1 have been identified to cause brachydactyly types B and …
Specific enolates from .alpha.-amino ketones (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSpecific enolates from .alpha.-amino ketonesMichael E. Garst, John N. Bonfiglio, David A. Grudoski, and Jeffrey MarksCite this: J. Org. Chem. 1980, 45, 12, 2307–2315Publication Date (Print):June 1, 1980Publication History Published online1 May 2002Published inissue 1 June 1980https://pubs.acs.org/doi/10.1021/jo01300a009https://doi.org/10.1021/jo01300a009research-articleACS PublicationsRequest reuse permissionsArticle Views1253Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Binding of actinomycin D to DNA: evidence for a nonclassical high-affinity binding mode that does not require GpC sites. (1989)

We have employed a combination of temperature-dependent UV absorption spectroscopy, circular dichroism, and batch calorimetry to characterize the binding of actinomycin D to a series of oligomeric DNA duplexes. We find the duplex [d(CGTCGACG)]2 to be unique in its ability to bind actinomycin D strongly despite the absence of a classic GpC site. We present evidence that this non-GpC-containing duplex binds two actinomycin D molecules in an apparently cooperative manner to form a complex that exhibits aberrant spectroscopic and calorimetric behavior. We propose that these observations are consistent with actinomycin D exhibiting a high-affinity, sequence-dependent DNA-binding mode distinct from its classic binding to isolated GpC sites.
Quantification in cardiac MRI: advances in image acquisition and processing (2010)

Cardiac magnetic resonance (CMR) imaging enables accurate and reproducible quantification of measurements of global and regional ventricular function, blood flow, perfusion at rest and stress as well as myocardial injury. Recent advances in MR hardware and software have resulted in significant improvements in image quality and a reduction in imaging time. Methods for automated and robust assessment of the parameters of cardiac function, blood flow and morphology are being developed. This article reviews the recent advances in image acquisition and quantitative image analysis in CMR.
Functional mapping of anti-factor IX inhibitors developed in patients with severe hemophilia B (2001)

Development of inhibitory antibodies is a serious complication of treatment with repeated factor IX infusions in a minority of patients with hemophilia B. Such antibodies detected in 8 patients have been characterized. Typing studies revealed that patients' immune response toward factor IX is highly heterogeneous and involves immunoglobulin G (IgG) antibodies, preferentially IgG1 and IgG4. The preservation of the sequence and the 3-dimensional orientation of the amino acids constituting one epitope are highly important for the assembly of an antibody-antigen complex. To localize the epitopes on the factor IX molecule, an original approach was designed using a set of factor X chimeras carrying regions of factor IX. Results showed that some patients' antibodies were directed against both the domain containing the gamma-carboxy glutamic acid residues (Gla domain) and the protease domain of factor IX. In contrast, no binding was observed to the epidermal growth factor-like domains or to the activation peptide. Functional characterization showed that the purified IgG from patients' serum inhibited the factor VIIIa-dependent activation of factor X. Moreover, patients' IgG directed against the Gla domain inhibited the binding of factor IX to phospholipids as well as the binding of factor VIII light chain to factor IXa. These data demonstrate that inhibitors appearing in patients with severe hemophilia B display specificity against restricted functional domains of factor IX.
Planning stable paths for concentric tube robots (2013)

Concentric tube robots are continuum robots that can navigate natural pathways to reach locations deep inside the human body. Their operation is based on rotating and telescopically actuating concentric tubes to achieve robot tip pose control. During tube manipulation, the elastic energy stored in the robot structure may give rise to unstable robot configurations and loss of control. This can occur, in particular, for highly curved and elongated tubes that are required for certain surgical interventions. This paper presents a path planning methodology that allows the utilization of such generally unstable concentric tube robots by ensuring that they operate in their stable configuration regions.
A Two-in-One antibody engineered from a humanized interleukin 4 antibody through mutation in heavy chain complementarity-determining regions (2014)

A mono-specific antibody may recruit a second antigen binding specificity, thus converting to a dual-specific Two-in-One antibody through mutation at the light chain complementarity-determining regions (CDRs). It is, however, unknown whether mutation at the heavy chain CDRs may evolve such dual specificity. Herein, we examined the CDRs of a humanized interleukin 4 (IL4) antibody using alanine scanning and structural modeling, designed libraries of mutants in regions that tolerate mutation, and isolated dual specific antibodies harboring mutation at the heavy chain CDRs only. We then affinity improved an IL4/IL5 dual specific antibody to variants with dissociation constants in the low nanomolar range for both antigens. The results demonstrate the full capacity of antibodies to evolve dual binding specificity.
Formation of Core/Shell Nanoparticles with a Lipid Core and Their Application as a Drug Delivery System (2005)

A novel preparation method for core/shell nanoparticles with a drug-loaded lipid core was designed and characterized. The lipid core is composed of lecithin and a drug, and the polymeric shell is composed of Pluronics (poly(ethylene oxide)-poly (propylene oxide)-poly(ethylene oxide) triblock copolymer, F-127). For the formation of stabilized core/shell nanoparticles, freeze-drying was performed in the presence of trehalose used as a cryoprotectant. Cryogenic transmittance electron microscopy (cryo-TEM), differential scanning calorimetry (DSC), and a particle size analyzer were used to observe the formation of the stabilized core/shell nanoparticles. For the application of the core/shell nanoparticles as a drug carrier, paclitaxel, a potent anticancer drug, was loaded into the core/shell nanoparticles, and the drug loading amount and the drug release pattern were observed.
Re-evaluating the role of BCR/ABL in chronic myelogenous leukemia (2014)

Chronic myelogenous leukemia (CML) requires the BCR/ABL tyrosine kinase for disease onset and maintenance. As a result, CML can be successfully treated with tyrosine kinase inhibitors (TKIs) such as imatinib. Most patients are maintained in a disease-suppressed state on daily TKI therapy for several years and in many cases this treatment prevents progression to the blast phase. If the TKI is discontinued, CML redevelops in 95% of patients as a result of persisting leukemia initiating cells (LICs). There are several hypotheses that describe the potential mechanism(s) responsible for LIC persistence in CML, but supporting evidence is limited. Furthermore, of the few patients who discontinue TKI therapy and are "cured" (i.e., in treatment-free remission), most have residual BCR/ABL-expressing cells in their hematopoietic tissues. There are also healthy individuals without a CML diagnosis who express the BCR/ABL mutation in a fraction of their hematopoietic cells. Finally, mice that express BCR/ABL from the Bcr locus as a knockin mutation do not develop CML. These mice have lower BCR/ABL levels than retroviral or transgenic models of BCR/ABL that do develop CML. Understanding why mice with BCR/ABL expressed from the Bcr locus and some people that express BCR/ABL are not afflicted with CML will provide insights into therapies to prevent or cure this disease.
The constitutive and stress inducible forms of hsp 70 exhibit functional similarities and interact with one another in an ATP-dependent fashion. (1993)

Mammalian cells constitutively express a cytosolic and nuclear form of heat shock protein (hsp) 70, referred to here as hsp 73. In response to heat shock or other metabolic insults, increased expression of another cytosolic and nuclear form of hsp 70, hsp 72, is observed. The constitutively expressed hsp 73, and stress-inducible hsp 72, are highly related proteins. Still unclear, however, is exactly why most eukaryotic cells, in contrast to prokaryotic cells, express a novel form of hsp 70 (i.e., hsp 72) after experiencing stress. To address this question, we prepared antibodies specific to either hsp 72 or hsp 73 and have compared a number of biological properties of the two proteins, both in vivo and in vitro. Using metabolic pulse-chase labeling and immunoprecipitation analysis, both the hsp 72 and hsp 73 specific antibodies were found to coprecipitate a significant number of newly synthesized proteins. Such interactions appeared transient and sensitive to ATP. Consequently, we suspect that both hsp 72 and hsp 73 function as molecular chaperones, interacting transiently with nascent polypeptides. During the course of these studies, we routinely observed that antibodies specific to hsp 73 resulted in the coprecipitation of hsp 72. Similarly, antibodies specific to hsp 72 were capable of coprecipitating hsp 73. Using a number of different approaches, we show that the constitutively expressed, pre-existing hsp 73 rapidly forms a stable complex with the newly synthesized stress inducible hsp 72. As is demonstrated by double-label indirect immunofluorescence, both proteins exhibit a coincident locale within the cell. Moreover, injection of antibodies specific to hsp 73 into living cells effectively blocks the ability of both hsp 73 and hsp 72 to redistribute from the cytoplasm into the nucleus and nucleolus after heat shock. These results are discussed as they relate to the possible structure and function of the constitutive (hsp 73) and highly stress inducible (hsp 72) forms of hsp 70, both within the normal cell as well as in the cell experiencing stress.
La Trombina más allá de la coagulación (Revisión) (2015)

espanolLa trombina es una proteasa de serina que resulta de la activacion proteolitica de protrombina, y cuya funcion principal en la coagulacion es la escision del fibrinogeno para formar el coagulo de fibrina. Paradojicamente, tambien interviene en la activacion del inhibidor de fibrinolisis activado por trombina y liberacion de activadores del plasminogeno, para la generacion de plasmina, con consiguiente degradacion del coagulo; estableciendose un equilibrio entre ambos mecanismos. Estudios tanto in vivo como in vitro han demostrado efectos de trombina mas alla del proceso de la coagulacion; ejerciendo acciones sobre monocitos, celulas endoteliales, celulas musculares lisas vasculares, plaquetas y neuronas, entre otras; que a su vez inciden directa o indirectamente sobre mecanismos inflamatorios, de proliferacion celular y nocicepcion. En ese sentido, la presente revision describe los efectos pleiotropicos o no hemostaticos de trombina relacionados con patologias inflamatorias, proliferativas; y percepcion del dolor nociceptivo y neuropatico. EnglishThrombin is a serine protease which results from the proteolytic activation of prothrombin, and whose main function in the process of clotting is the cleavage of fibrinogen to form the fibrin clot. Paradoxically, it is also involved in the activation of the fibrinolysis inhibitor activated by thrombin and the release of plasminogen activators to plasmin generation, with consequent degradation of the clot; establishing a balance between the two mechanisms. Studies both in vivo and in vitro have shown effects beyond the thrombin clotting process; exerting actions on monocytes, endothelial cells, vascular smooth muscle cells, neurons and platelets, among others; which in turn act directly or indirectly on inflammatory mechanisms of cell proliferation and nociception. In that sense, the present review describes the pleiotropic effects or related thrombin hemostatic inflammatory, proliferative diseases; and perception of nociceptive and neuropathic pain.
Bacterial DNA Translocation Is Associated With Systemic Circulatory Abnormalities and Intrahepatic Endothelial Dysfunction in Patients With Cirrhosis (2010)

Presence of bacterial DNA, a marker of bacterial translocation, is associated with aggravation of peripheral vasodilation and with worsening of intrahepatic endothelial dysfunction.