gebhart/patrepeval huggingface dataset:
The Role of Polyamine Architecture on the Pharmacological Activity of Open Lactone Camptothecin−Polyamine Conjugates (2008)

A series of camptothecin open-ring lactone tripartate conjugates were synthesized, in which polyamine side chains with different architecture (ethane-1,2-diamine, spermidine, homospermidine, spermine, and 4,8,13,17-tetraza-icosane-1,20-diamine) are linked to the 21-carboxylic function through an amidic bond, while the 17-CH(2)OH is acetylated. The rationale for the synthesis of these compounds was to explore the influence of the polyamine architecture on the activity of these CPT conjugates into cells, since the positively charged ammonium cations would favor interaction through electrostatic binding to the negatively charged DNA backbone. Topoisomerase I-mediated DNA cleavage assay was used to investigate the ability of these compounds to stimulate the DNA damage. The cleavage pattern was found to be similar to that of SN38 for all the new CPTs. The CPT tripartates were tested for growth inhibition ability against the human non-small-cell lung cancer carcinoma NCI-H460 cell line. Although these compounds were less potent than topotecan, SN38, and CPT after 1 h of treatment, the antiproliferative effects greatly increased after 72 h of exposure. The growth inhibition potency during long-term exposure is correlated with the number of charges of the 21-amide substituent. Both cleavage assay and in vitro effects support the interpretation that the compounds may have inhibitory activity also in the open-ring form. The architecture of the polyamine moiety is important for antiproliferative activity, and a balance between the hydrophilic and lipophilic properties of the polyamine is critical for CPT potency.
Extraction of Indigo dye from Couroupita guianensisand its application on cotton fabric (2014)

Abstract Couroupita guianensis (Cannon ball tree) flowers and fruits are known to contain indigotin and indirubin. In this study an attempt is made to optimize the period for effective extraction of indigo from Couroupita guianensis fruit and application of the crude extract on the cotton fabric. Fermentation of the Couroupita guianensis fruit pulp for 5 days gave intense bluer pigment and hence was selected as the optimum period for fermentation. Major colouring components from Couroupita guianensis fruit extract were separated by thin layer chromatography using single solvent system, chloroform; which gives three pigments, violet, blue and pink with the R f values of 0.88, 0.57, and 0.25 respectively. The R f values, the wavelength of maximum absorption from UV-Visible spectroscopy, Fourier Transform Infrared (FTIR) spectrum and 1 H Nuclear magnetic resonance ( 1 H NMR) spectrum of blue pigment matched with that of synthetic indigo standard and that of the pink pigment matched with reported values of indirubin which is an isomer of indigotin. The % purity of indigo dye was found to be 26.46%. Dyeing of cotton fabric was carried out with the crude dye powder which gave comparable fastness properties vis-a-vis synthetic indigo.
LibriTTS: A Corpus Derived from LibriSpeech for Text-to-Speech (2019)

This paper introduces a new speech corpus called "LibriTTS" designed for text-to-speech use. It is derived from the original audio and text materials of the LibriSpeech corpus, which has been used for training and evaluating automatic speech recognition systems. The new corpus inherits desired properties of the LibriSpeech corpus while addressing a number of issues which make LibriSpeech less than ideal for text-to-speech work. The released corpus consists of 585 hours of speech data at 24kHz sampling rate from 2,456 speakers and the corresponding texts. Experimental results show that neural end-to-end TTS models trained from the LibriTTS corpus achieved above 4.0 in mean opinion scores in naturalness in five out of six evaluation speakers. The corpus is freely available for download from http://www.openslr.org/60/.
Laser annealing of silicon on sapphire (1979)

Silicon-implanted silicon-on-sapphire wafers have been annealed by 50-ns pulses from a Q-switched Nd : YAG laser. The samples have been analyzed by channeling and by ω-scan x-ray double diffraction. After irradiation with pulses of a fluence of about 5 J cm−2 the crystalline quality of the silicon layer is found to be better than in the as-grown state.
Specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses (2008)

Human metapneumovirus (HMPV) and avian metapneumovirus (AMPV) have a similar genome organization and protein composition, but a different host range. AMPV subgroup C (AMPV-C) is more closely related to HMPV than other AMPVs. To investigate the specificity and functional interaction of the polymerase complex proteins of human and avian metapneumoviruses, a minireplicon system was generated for AMPV-C and used in combination with minireplicon systems for HMPV lineages A1 and B1. Viral RNA-like molecules representing HMPV-A1 and -B1, AMPV-A and -C and human respiratory syncytial virus were replicated efficiently by polymerase complexes of HMPV-A1 and -B1 and AMPV-C, but not by polymerase complexes of bovine parainfluenza virus 3. Upon exchange of HMPV and AMPV-C polymerase complex components, all chimeric polymerase complexes were functional; exchange between HMPVs did not result in altered polymerase activity, whereas exchange between HMPVs and AMPV-C did. Recombinant HMPV-B1 viruses in which polymerase genes were exchanged with those of HMPV-A1 replicated with normal kinetics in vitro, whilst replacement with AMPV-C genes resulted in moderate differences in virus replication. In hamsters, recombinant HMPV-B1 viruses in which individual polymerase genes were exchanged with those of AMPV-C were attenuated, irrespective of the results obtained with minireplicon systems or in vitro replication assays. This study provides insight into the specificity and functional interaction of polymerase complex proteins of human and avian metapneumoviruses, but neither minireplicon systems nor in vitro replication kinetics were found to be predictive for attenuation in permissive animals.
A prostate-specific antigen doubling time of <6 months is prognostic for metastasis and prostate cancer-specific death for patients receiving salvage radiation therapy post radical prostatectomy (2013)

A pre-SRT PSADT <6 months was a strong predictor of outcomes in our data set, including PCSM. The most common nomogram for SRT uses a 10-month PSADT threshold for assigning points used to assess BF following SRT. If validated, our findings suggest that a PSADT threshold of <6 months should be considered for stratification of patients in future clinical trials in this setting.
Essential fatty acid deficiency ameliorates acute renal dysfunction in the rat after the administration of the aminonucleoside of puromycin. (1990)

The administration of the aminonucleoside of puromycin (PAN) to rats causes the nephrotic syndrome that is associated with an acute decline in renal function, and an interstitial infiltrate. We examined whether essential fatty acid deficiency (EFAD), which inhibits macrophage infiltration in glomerulonephritis, affects PAN-induced renal dysfunction. Both control and EFAD rats developed proteinuria that resolved over 28 d. After PAN administration, there was a prominent infiltration of macrophages in rats fed a normal diet. The infiltrate was prevented by the EFAD diet. The absence of a macrophage interstitial infiltrate was associated with a significantly higher Cin in the EFAD rats than in controls at 7 d (5.21 +/- 1.19 versus 0.39 +/- 0.08, P less than 0.002 ml/min/kg BW). In addition, CPAH fell to less than 10 ml/min/kg BW by day 7 in controls, but remained the same as normal in the EFAD. After administration of PAN to control rats, there was no increase in urinary thromboxane excretion or an increase in glomerular thromboxane production. Furthermore, the effect of EFAD could not be mimicked by the administration of a thromboxane synthase inhibitor. Irradiation-induced leukopenia in rats on a normal diet markedly improved glomerular filtration and renal blood flow in acutely nephrotic rats. EFAD prevents the interstitial cellular infiltrate and the renal ischemia associated with experimental nephrosis. The recruitment of mononuclear cells into the kidney following PAN directly contributes to the decline in renal function.
A Software Approach to Improving SEM Resolution, Image Quality, and Productivity (2017)

Over the years, significant advances have been made in SEM resolution largely attributable to hardware improvements, including higher brightness sources, better electron optics, and more efficient detectors. The underlying rationale is that reduced probe diameter leads to better resolution. These changes are significant, but they are generally accompanied with increased complexity and cost. While a small probe diameter may be a necessary condition for high resolution, it may not be sufficient for a number of reasons. First, although the probe diameter may be minimized at a particular point of impact, it will not be constant along the beam axis leading to depth of focus issues at very high magnification. Second, the signal measured may originate from some depth below the point of impact, and the actual excitation volume may have dimensions that greatly exceed the minimum probe diameter. This difficulty may be partially overcome by choosing a particular signal type like secondary electrons (SE) over backscattered electrons (BSE) at high accelerating voltages, however even SE images may have limitations if the mean-free path of the secondary electrons is significantly larger than the probe diameter. Gold on carbon is often the standard of choice for resolution tests because both the excitation volume and mean-free path of SEs can be small relative to the probe diameter, however this would not be the case for a fine carbon structure on gold. It is now well recognized that issues relating to excitation volume, particularly for BSEs, can be reduced by going to low beam energies, often 2 keV or less, and this is particularly important in the examination of biological samples and microelectronic devices. Low-voltage operation is, however, often accompanied by larger probe sizes and reduced probe currents. A third critical factor, therefore, is probe current. It must be large for a high signal-to-noise ratio (S/N). If the S/N is too low, fine details may be lost in the noise. Although the S/N of an image can be improved by increasing the image collection time, this can lead to decreased productivity as well as other problems including sample drift, contamination, and vibrations.
Act1 Adaptor Protein Is an Immediate and Essential Signaling Component of Interleukin-17 Receptor (2006)

Interleukin (IL)-17, the founding member of the IL-17 cytokine family, is the hallmark of a novel subset of CD4+ T cells that is regulated by TGFbeta, IL-6, and IL-23. IL-17 plays an important role in promoting tissue inflammation in host defense against infection and in autoimmune diseases. Although IL-17 has been reported to regulate the expression of proinflammatory cytokines, chemokines, and matrix metalloproteinases, the signaling mechanism of IL-17 receptor has not been understood. An earlier study found that IL-17 activates NF-kappaB and MAPK pathways and requires TRAF6 to induce IL-6. However, it is unknown what molecule(s) directly associates with IL-17 receptor to initiate the signaling. We demonstrate here that IL-17 receptor family shares sequence homology in their intracellular region with Toll-IL-1 receptor (TIR) domains and with Act1, a novel adaptor previously reported as an NF-kappaB activator. MyD88 and IRAK4, downstream signaling components of TIR, are not required for IL-17 signaling. On the other hand, Act1 and IL-17 receptor directly associate likely via homotypic interaction. Deficiency of Act1 in fibroblast abrogates IL-17-induced cytokine and chemokine expression, as well as the induction of C/EBPbeta, C/EBPdelta, and IkappaBzeta. Also, absence of Act1 results in a selective defect in IL-17-induced activation of NF-kappaB pathway. These results thus indicate Act1 as a membrane-proximal adaptor of IL-17 receptor with an essential role in induction of inflammatory genes. Our study not only for the first time reveals an immediate signaling mechanism downstream of an IL-17 family receptor but also has implications in therapeutic treatment of various immune diseases.
OmniDepth: Dense Depth Estimation for Indoors Spherical Panoramas (2018)

Recent work on depth estimation up to now has only focused on projective images ignoring 360 content which is now increasingly and more easily produced. We show that monocular depth estimation models trained on traditional images produce sub-optimal results on omnidirectional images, showcasing the need for training directly on 360 datasets, which however, are hard to acquire. In this work, we circumvent the challenges associated with acquiring high quality 360 datasets with ground truth depth annotations, by re-using recently released large scale 3D datasets and re-purposing them to 360 via rendering. This dataset, which is considerably larger than similar projective datasets, is publicly offered to the community to enable future research in this direction. We use this dataset to learn in an end-to-end fashion the task of depth estimation from 360 images. We show promising results in our synthesized data as well as in unseen realistic images.
Advanced Cold Rolled Steels for Automotive Applications (2009)

Advanced high-strength steels offer a great potential for the further development of automobile bodies-in-white due to their combined mechanical properties of high formability and strength. They represent the first choice in material selection for strength and crash-relevant parts with challenging geometries. The intensive development of multiphase steels by ThyssenKrupp Steel has led to hot dip galvanizing concepts with an outstanding forming potential. Hot rolled, hot dip galvanized complex-phase steels are currently produced in addition to cold rolled dual phase (DP) and retained austenite (RA) or transformation induced plasticity (TRIP) steels.



New continuously annealed grades of steel are being developed with tensile strength levels of up to 1000 MPa in combination with sufficient ductility for the high demands of structural automobile components. These steels make use of the classic advantages of microalloying as well as the principles of DP steels and RA / TRIP steels. Further improvement of properties will be reached by the new class of high manganese alloyed steels.
Potent sirtuin inhibition bestowed by l-2-amino-7-carboxamidoheptanoic acid (l-ACAH), a Nε-acetyl-lysine analog (2011)

Inhibitors of sirtuin-catalyzed NAD+-dependent proteinlysine deacetylation reaction possess great value for a fuller exploration of sirtuin biology/pharmacology and as potential therapeutic agents for metabolic and age-related diseases and cancer. In the current study, we discovered that L-2-amino-7-carboxamidoheptanoic acid (L-ACAH), a Nε-acetyl-lysine analog, could confer potent SIRT1 inhibition in both the peptidic and the peptidomimetic inhibitors. This inhibition was demonstrated to be mechanism-based since the in vitro studies with the peptidic inhibitor revealed that (i) the inhibitor was competitive versus the acetyl-lysine substrate, (ii) the inhibitor was of the slow, tight-binding type, and (iii) the inhibitor was able to be converted to at least one longer-lived catalytic intermediate and perhaps all the way to the product (similar to 2′-O-acetyl-ADP-ribose) as well. The in vivo characterization of the peptidomimetic inhibitor revealed its capability of inhibiting the SIRT1 inside the human colon cancer cell line HCT116. This peptidomimetic compound was also shown to be an in vitroinhibitor against SIRT2 and SIRT3 with comparable potency to that against SIRT1. These results point to the potential of developing further potent cell permeable L-ACAH-based inhibitors for sirtuins.
Development and Evaluation of Real-Time Volumetric Compton Gamma-Ray Imaging (2016)

An approach to gamma-ray imaging has been developed that enables near real-time volumetric (3D) imaging of unknown environments thus improving the utility of gamma-ray imaging for source-search and radiation mapping applications. The approach, herein dubbed scene data fusion (SDF), is based on integrating mobile radiation imagers with real-time tracking and scene reconstruction algorithms to enable a mobile mode of operation and 3D localization of gamma-ray sources. The real-time tracking allows the imager to be moved throughout the environment or around a particular object of interest, obtaining the multiple perspectives necessary for standoff 3D imaging. A 3D model of the scene, provided in real-time by a simultaneous localization and mapping (SLAM) algorithm, can be incorporated into the image reconstruction reducing the reconstruction time and improving imaging performance. The SDF concept is demonstrated in this work with a Microsoft Kinect RGB-D sensor, a real-time SLAM solver, and two different mobile gamma-ray imaging platforms. The first is a cart-based imaging platform known as the Volumetric Compton Imager (VCI), comprising two 3D position-sensitive high purity germanium (HPGe) detectors, exhibiting excellent gamma-ray imaging characteristics, but with limited mobility due to the size and weight of the cart. The second system is the High Efficiency Multimodal Imager (HEMI) a hand-portable gamma-ray imager comprising 96 individual cm$^{3}$ CdZnTe crystals arranged in a two-plane, active-mask configuration. The HEMI instrument has poorer energy and angular resolution than the VCI, but is truly hand-portable, allowing the SDF concept to be tested in multiple environments and for more challenging imaging scenarios. An iterative algorithm based on Compton kinematics is used to reconstruct the gamma-ray source distribution in all three spatial dimensions. Each of the two mobile imaging systems are used to demonstrate SDF for a variety of scenarios, including general search and mapping scenarios with several point gamma-ray sources over the range of energies relevant for Compton imaging. More specific imaging scenarios are also addressed, including directed search and object interogation scenarios. Finally, the volumetric image quality is quantitatively investigated with respect to the number of Compton events acquired during a measurement, the list-mode uncertainty of the Compton cone data, and the uncertainty in the pose estimate from the real-time tracking algorithm. SDF advances the real-world applicability of gamma-ray imaging for many search, mapping, and verification scenarios by improving the tractiblity of the gamma-ray image reconstruction and providing context for the 3D localization of gamma-ray sources within the environment in real-time.
Insulins in equine urine: qualitative analysis by immunoaffinity purification and liquid chromatography/tandem mass spectrometry for doping control purposes in horse‐racing (2008)

Insulin is a peptide hormone consisting of two peptide chains (A- and B-chain) that are cross-linked by two disulfide bonds. To obtain improved pharmacokinetic onset of action profiles of insulin treatment in diabetic patients, recombinant long-, intermediate-, and rapid-acting insulin analogs are produced, in which the C-terminal end of the B-chain plays an especially important role.A review of the veterinary literature reveals the low prevalence of equine type I diabetes mellitus, which indicates that the therapeutic use of insulin in racing horses is unlikely. Although there is no unequivocal evidence of an overall performance-enhancing effect of insulin, in human sports the misuse of insulin preparations is reported among elite athletes. The desired effects of insulin include the increase of muscular glycogen prior to sports event or during the recovery phase, in addition to a chalonic action, which increases the muscle size by inhibiting protein breakdown. In the present study urinary insulin was detected in equine samples and differences between equine insulin, human insulin, as well as rapidly acting recombinant insulin variants were examined. The method was based on sample purification by solid-phase extraction (SPE) and immunoaffinity chromatography (IAC), and subsequent analysis by microbore liquid chromatography (LC) and tandem mass spectrometry (MS/MS) using top-down sequencing for the determination of various insulins. Product ion scan experiments of intact proteins and B-chains enabled the differentiation between endogenously produced equine insulin, its DesB30 metabolite, human insulin and recombinant insulin analogs, and the assay allowed the assignment of individual product ions, especially those originating from modified C-termini of B-chains.
1/F Noise and Auditory Aesthetics: Sonification of a Driven Bead Pile (2011)

Presented at the 17th International Conference on Auditory Display (ICAD2011), 20-23 June, 2011 in Budapest, Hungary.
Preliminary DTV field test results and their effects on VSB receiver design (1999)

The Federal Communication Commission (FCC) has selected the Advanced Television System Committee (ATSC) digital television (DTV) standard (except for video formats) as the new terrestrial standard for the United States. The U.S. has begun the transition from the 50+-year old analog NTSC system to the new digital ATSC system. A brief overview of ATSC's digital vestigial sideband (VSB) transmission system is covered, as well as the methodology used in field-test data gathering and data analysis. Results from DTV field tests in several U.S. cities by early adopter DTV broadcasters are summarized. Finally, FCC planning factors for consumer DTV receivers that are used in allocating spectrum for the 1600+ broadcast stations based on NTSC replication are discussed along with digital VSB receiver target design parameters that were developed as a result of these VSB field tests.
Draft Genome Sequences of Paenibacillus polymyxa NRRL B-30509 and Paenibacillus terrae NRRL B-30644, Strains from a Poultry Environment That Produce Tridecaptin A and Paenicidins (2015)

Paenibacillus polymyxa NRRL B-30509 and Paenibacillus terrae NRRL B-30644 produce tridecaptin A that is inhibitory to Campylobacter jejuni, as well as lantibiotics in the paenicidin family. Here, we report the draft genome sequences of P. polymyxa NRRL B-30509 and P. terrae NRRL B-30644 that contain gene clusters for various nonribosomal lipopeptides.