gebhart/patrepeval huggingface dataset:
BamE Modulates the Escherichia coli Beta-Barrel Assembly Machine Component BamA (2011)

Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins BamA and BamD and three nonessential lipoproteins, BamB, BamC, and BamE. Both BamC and BamE are important for stabilizing the interaction between BamA and BamD. We used comprehensive genetic analysis to clarify the interplay between BamA and the BamCDE subcomplex. Combining a ΔbamE allele with mutations in genes that encode other OMP assembly factors leads to severe synthetic phenotypes, suggesting a critical function for BamE. These synthetic phenotypes are not nearly as severe in a ΔbamC background, suggesting that the functions of BamC and BamE are not completely overlapping. This unique function of BamE is related to the conformational state of BamA. In wild-type cells, BamA is sensitive to externally added proteinase K. Strikingly, when ΔbamE mutant cells are treated with proteinase K, BamA is degraded beyond detection. Taken together, our findings suggest that BamE modulates the conformation of BamA, likely through its interactions with BamD.
The Meanings of Medicating: Pills and Play (2005)

The author uses Winnicott's theory involving transitional objects, the therapeutic value of play, and the vital role of danger to elucidate the psychotherapeutic aspects of medication visits. A case vignette illustrates growth of the possibility of play in the course of visits whose overt agenda is "meds only."
Antigenic role of single residues within the main immunogenic region of the nicotinic acetylcholine receptor (1990)

The target of most of the autoantibodies against the acetylcholine receptor (AChR) in myasthenic sera is the main immunogenic region (MIR) on the extracellular side of the AChR alpha-subunit. Binding of anti-MIR monoclonal antibodies (mAbs) has been recently localized between residues alpha 67 and alpha 76 of Torpedo californica electric organ (WNPADYGGIK) and human muscle (WNPDDYGGVK) AChR. In order to evaluate the contribution of each residue to the antigenicity of the MIR, we synthesized peptides corresponding to residues alpha 67-76 from Torpedo and human AChRs, together with 13 peptide analogues. Nine of these analogues had one residue of the Torpedo decapeptide replaced by L-alanine, three had a structure which was intermediate between those of the Torpedo and human alpha 67-76 decapeptides, and one had D-alanine in position 73. Binding studies employing six anti-MIR mAbs and all 15 peptides revealed that some residues (Asn68 and Asp71) are indispensable for binding by all mAbs tested, whereas others are important only for binding by some mAbs. Antibody binding was mainly restricted to residues alpha 68-74, the most critical sequence being alpha 68-71. Fish electric organ and human MIR form two distinct groups of strongly overlapping epitopes. Some peptide analogues enhanced mAb binding compared with Torpedo and human peptides, suggesting that the construction of a very antigenic MIR is feasible.
T <sub>reg</sub> Cells in Cancer: A Case of Multiple Personality Disorder (2012)

Foxp3(+) RORγt-expressing T cells expand in colorectal cancer and contribute to pathogenesis in a mouse model of polyposis.
Hepatic lipase facilitates the selective uptake of cholesteryl esters from remnant lipoproteins in apoE-deficient mice (1998)

We have investigated the role of hepatic lipase (HL) in remnant lipoprotein metabolism independent of lipolysis by using recombinant adenovirus to express native and catalytically inactive HL (HL-145G) in apolipoprotein (apo)E-deficient mice characterized by increased plasma concentrations of apoB-48-containing remnants. In the absence of apoE, the mechanisms by which apoB-48-containing remnants are taken up by either low density lipoprotein (LDL)-receptor or LDL-receptor-related protein (LRP) remain unclear. Overexpression of either native or catalytically inactive HL in apoE-deficient mice led to similar reductions (P > 0.5) in the plasma concentrations of cholesterol (41% and 53%) and non high density lipoprotein (HDL)-cholesterol (41% and 56%) indicating that even in the absence of lipolysis, HL can partially compensate for the absence of apoE in this animal model. Although the clearance of [3H]cholesteryl ether from VLDL was significantly increased (approximately 2-fold; P < 0. 02) in mice expressing native or inactive HL compared to luciferase controls, the fractional catabolic rates (FCR) of [125I-labeled] apoB- very low density lipoprotein (VLDL) in all three groups of mice were similar (P > 0.4, all) indicating selective cholesterol uptake. Hepatic uptake of [3H]cholesteryl ether from VLDL was greater in mice expressing either native HL (87%) or inactive HL-145G (72%) compared to luciferase controls (56%). Our combined findings are consistent with a role for HL in mediating the selective uptake of cholesterol from remnant lipoproteins in apoE-deficient mice, independent of lipolysis. These studies support the concept that hepatic lipase (HL) may serve as a ligand that mediates the interaction between remnant lipoproteins and cell surface receptors and/or proteoglycans. We hypothesize that one of these pathways may involve the interaction of HL with cell surface receptors, such as scavenger receptor (SR)-BI, that mediate the selective uptake of cholesteryl esters.
Designing multiplane computer-generated holograms with consideration of the pixel shape and the illumination wave (2008)

The majority of image-generating computer-generated holograms (CGHs) are calculated on a discrete numerical grid, whose spacing is defined by the desired pixel size. For single-plane CGHs the influence of the pixel shape and the illumination wave on the actual output distribution is minor and can be treated separately from the numerical calculation. We show that in the case of multiplane CGHs this influence is much more severe. We introduce a new method that takes the pixel shape into account during the design and derive conditions to retain an illumination-wave-independent behavior.
Localization of glycosylation sites in the golgi apparatus using immunolabeling and cytochemistry (1991)

This review summarizes data on the distribution of certain glycosylation steps in the Golgi apparatus as revealed by immunolabeling and lectin techniques. The methodical basis for such investigations was provided by the introduction of the colloidal gold marker system for immunolabeling and the development of new means of tissue processing such as the low-temperature embedding technique using Lowicryl K4M. The application of these techniques together with highly specific antibodies has provided much of the basis for our current understanding of the Golgi apparatus in functional terms. Thus, in many cell types, three Golgi apparatus compartments can be distinguished, whereas in others no such functional subdivision is evident. Investigations on sialyltransferase distribution have also provided direct evidence that GERL is structurally and functionally part of the Golgi apparatus.
An in vitro model of lymphocyte homing. I. Characterization of the interaction between thoracic duct lymphocytes and specialized high-endothelial venules of lymph nodes. (1977)

An in vitro system was used to study lymphocyte interaction with high-endothelial venules (HEV) in rat lymph nodes. The results show that thoracic duct lymphocytes (TDL) are capable of selective adherence to the endothelium of HEV when overlaid onto glutaraldehyde-fixed sections of lymph nodes. At optimal TDL concentration (30 × 10 6 /ml) and incubation temperature (7°C) binding occurs rapidly and the majority of HEV in each section usually shows adherent lymphocytes, the number attaching to each vessel being proportional to the endothelial area. TDL do not bind to other vascular structures in the node and ordinarily there is little adherence to other cells in the section. The results indicate therefore that TDL have surface membrane structures specific for HEV recognition. Although TDL adhere to glutaraldehyde-fixed HEV, lymphocytes pretreated with fixative lose their capacity to bind. Also incubation performed at 1°C produces a fairly marked reduction in binding capacity of normal TDL. These observations indicate that endothelium of HEV are passive participants in the in vitro reaction whereas metabolic activity is important on the part of lymphocytes. In addition, when incubation is performed at 24°C, binding is severely reduced and at 37°C few, if any, HEV exhibit adherent TDL. It is suggested that lymphocytes that adhere to HEV are capable of rapid dissociation under physiologic conditions, a property that could facilitate entry of lymphocytes into the lymph node after their interaction with the specialized endothelium in vivo .
Spatio-temporal Compressed Sensing with Coded Apertures and Keyed Exposures (2011)

Optical systems which measure independent random projections of a scene according to compressed sensing (CS) theory face a myriad of practical challenges related to the size of the physical platform, photon efficiency, the need for high temporal resolution, and fast reconstruction in video settings. This paper describes a coded aperture and keyed exposure approach to compressive measurement in optical systems. The proposed projections satisfy the Restricted Isometry Property for sufficiently sparse scenes, and hence are compatible with theoretical guarantees on the video reconstruction quality. These concepts can be implemented in both space and time via either amplitude modulation or phase shifting, and this paper describes the relative merits of the two approaches in terms of theoretical performance, noise and hardware considerations, and experimental results. Fast numerical algorithms which account for the nonnegativity of the projections and temporal correlations in a video sequence are developed and applied to microscopy and short-wave infrared data.
Tyrosine 92 of aristolochene synthase directs cyclisation of farnesyl pyrophosphateElectronic supplementary information (ESI) available: GC-profiles of co-injections of 4 from ASY92V and authentic material and mass spectra of 4 produced by ASY92V and an authentic sample. See http://www.rsc.org/suppdata/cc/b2/b206517g/ (2002)

A mutant of Aristolochene Synthase (AS), in which Tyr 92 was replaced by Val, produced the alicyclic beta-(E)-farnesene as the major product, indicating that cyclisation of FPP is controlled by Tyr 92 in AS.
Neural network step-frequency fault locator (1995)

A step-frequency fault locator was constructed as a new diagnostic tool for integrated optics devices. The wavelength of the light was swept stepwise from 1.5 to 1.6 μm in steps less than 1 nm. The image of the target was processed using a neural network algorithm. Detailed comparisons between the results using the neural network algorithm and FFT algorithm have been presented. Obtained resolution was of the order of microns. The overall dimensions were reduced to 7 x l0 x 2 in. by replacing bulk optic elements with fiber-optic elements.
Catalytic Properties of Silica-supported 12-Molybdophosphoric Acid in the Conversion of 2-Propanol (1995)

A technical silica gel was impregnated with a solution of the titled heteropoly acid (1–20 w/w%) and calcined at 400, 600 or 800 °C. Dehydration of the alcohol generally increased with the loaded acid, and decreased almost linearly with the reaction temperature (360–260°C). The best alcohol decomposition was achieved over the 600°C-calcined catalysts when over 90% conversion to propene was attained. The apparent activation energies decreased with the degree of loading and attained a value of 22 kJ/mol on the 600°C-calcined 20% HPMo-supported catalyst (half that on the solids calcined at 400°C and 800°C). The catalytic dehydration cannot be described by means of a proton-involved mechanism on the severely treated catalysts at ≥600°C. An outer surface mechanism might be effective involving terminal oxygens and MoV ions.
Spin Labeling and Kinetic Studies of a Membrane‐Immobilized Proteolytic Enzyme (1992)

Membrane-based bioreactors can greatly influence the rate and extent of chemical reactions and consequently lower the costs associated with the corresponding engineering processes. However, in order to progress in this area, greater understanding of the relationship of the structure and function of bioreactor systems is required. In this study, a proteolytic enzyme, papain (EC 3.4.22.2), was covalently coupled onto the surface of a vinyl alcohol/vinyl butyral copolymer (PVB) membrane employing either glutaraldehyde (GA) or 1,1'-carbonyldiimidazole (CDI). Various kinetic and performance properties of the immobilized papain were studied. It was found that these characteristics of the membrane-bound papain depended on the immobilization method. The CDI-immobilized papain bioreactor was used, although the apparent Michaelis constant, Km, of the CDI-immobilized papain was larger than that of the GA-immobilized enzyme. In separate experiments, a six-carbon spacer was also used between the membrane support and the covalently-linked enzyme. It was found that the insertion of the spacer reduced the disturbance of the enzyme system, resulting in a decreased Km, which was now closer to the value for the free enzyme. Electron paramagnetic resonance (EPR) techniques of spin labeling were used for the first time to examine the conformational change and the active site structure of an enzyme covalently immobilized to a membrane. The structural changes of the active site of papain upon immobilization with and without a spacer were in agreement with the functional properties of the enzyme.
Simultaneous Synthesis of Ozone and Hydrogen Peroxide in a Proton‐Exchange‐Membrane Electrochemical Reactor (1994)

Deionized water was oxidized to form ozone at the anode while oxygen was reduced to hydrogen peroxide at the cathode in a proton‐exchange‐membrane electrochemical flow reactor. The conditions for simultaneous generation of these oxidants were determined as a function of the applied voltage, electrode materials (lead dioxide for ozone evolution; gold, carbon, or graphite for peroxide evolution), and electrode configurations. Measured and calculated quantities included cell current, liquid‐ and gas‐phase ozone concentrations, hydrogen peroxide concentrations, current efficiency for ozone and peroxide evolution, and ozone and peroxide production rates. An applied potential of 4.5 V resulted in a current density of 2 A/cm2, yielding maximum gas‐ and liquid‐phase ozone concentrations of 60 and 3.1 mg/liter at the anode (4.5% current efficiency) and hydrogen peroxide concentrations between 3 and 5 mg/liter at the cathode (0.8% current efficiency).
An advanced multiplexed streamer system (1982)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 1982An advanced multiplexed streamer systemAuthors: Byron CainDale EzellJim SweetmanJack ThomsonLynn HeitmanByron CainGeophysical Service Inc., Dale EzellGeophysical Service Inc., Jim SweetmanGeophysical Service Inc., Jack ThomsonGeophysical Service Inc., and Lynn HeitmanGeophysical Service Inc.https://doi.org/10.1190/1.1826851 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1826851FiguresReferencesRelatedDetails SEG Technical Program Expanded Abstracts 1982ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 1982 Pages: 520 publication data© 1982 Copyright © 1982 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished: 24 Mar 2005 CITATION INFORMATION Byron Cain, Dale Ezell, Jim Sweetman, Jack Thomson, and Lynn Heitman, (1982), "An advanced multiplexed streamer system," SEG Technical Program Expanded Abstracts : 134-136. https://doi.org/10.1190/1.1826851 Plain-Language Summary PDF DownloadLoading ...
Helicobacter pylori as a protective factor against food allergy. (2008)

We conclude that: 1) Hp infection is associated with a decreased risk for food allergy; 2) presence of Hp in food allergy patients has ameliorating effect on the production of allergy mediators such as ECP and mast cell tryptase and 3) Hp infection appears to be a protective factor against food allergy.
Transmit Power Optimization and Feasibility Analysis of Self-Backhauling Full-Duplex Radio Access Systems (2018)

We analyze an inband full-duplex access node that is serving mobile users while simultaneously connecting to a core network over a wireless backhaul link, utilizing the same frequency band for all communication tasks. Such wireless self-backhauling is an intriguing option for the next generation wireless systems since a wired backhaul connection might not be economically viable if the access nodes are deployed densely. In particular, we derive the optimal transmit power allocation for such a system in closed form under quality-of-service (QoS) requirements, which are defined in terms of the minimum data rates for each mobile user. For comparison, the optimal transmit power allocation is solved also for two reference scenarios: a purely half-duplex access node, and a relay-type full-duplex access node. Based on the obtained expressions for the optimal transmit powers, we then show that the systems utilizing a full-duplex capable access node have a fundamental feasibility boundary, meaning that there are circumstances under which the QoS requirements cannot be fulfilled using finite transmit powers. This fundamental feasibility boundary is also derived in closed form. The feasibility boundaries and optimal transmit powers are then numerically evaluated in order to compare the different communication schemes. In general, utilizing the purely full-duplex access node results in the lowest transmit powers for all the communicating parties, although there are some network geometries under which such a system is not capable of reaching the required minimum data rates. In addition, the numerical results indicate that a full-duplex capable access node is best suited for relatively small cells.
Topical Conference on High Temperature Plasma Diagnostics, 7th, Napa, CA, Mar. 13-17, 1988, Proceedings (1988)

Various papers concerning scientific instruments are presented. The general topics addressed include: laser scattering and optical diagnostics, collective scattering and interferometry, millimeter wave and current profile measurements, particle-based diagnostics, data acquisition and analysis, X-ray diagnostics, and particle- and photon-based diagnostics. Individual subjects discussed include: atomic hydrogen density measurements in an ion source plasma using VUV absorption spectrometer, resonant diagnostics of laser-produced Ba plasmas, radiative and diffusional effects to the population densities of the excited-state atoms in hydrogen plasma, and Watt-level millimeter-wave monolithic diode-grid frequency multipliers.
The Use of Microorganisms To Increase the Recovery of Oil From Cores (1985)

ABSTRACT A method for oil recovery enhancement by stimulating the growth of indigenous microbial populations in sandstones by nutrient injection was investigated. Incremental oil recovery from the cores ranged from 10–38% of the original oil in place. Volumetric and microscopic sweep efficiency was improved by the microbial selective plugging process. Biogenic gas production accompanied the oil produced from the cores. The combination of stratification correction, gas production, and pressure increase assisted in recovery of previously trapped oil.
Improved Corners with Multi‐Channel Signed Distance Fields (2017)

Abstract We propose an extension to the state‐of‐the‐art text rendering technique based on sampling a 2D signed distance field from a texture. This extension significantly improves the visual quality of sharp corners, which is the most problematic feature to reproduce for the original technique. We achieve this by using a combination of multiple distance fields in conjunction, which together provide a more thorough representation of the given glyph's (or any other 2D shape's) geometry. This multi‐channel distance field representation is described along with its application in shader‐based rendering. The rendering process itself remains very simple and efficient, and is fully compatible with previous monochrome distance fields. The introduced method of multi‐channel distance field construction requires a vector representation of the input shape. A comparative measurement of rendering quality shows that the error in the output image can be reduced by up to several orders of magnitude.
The dynamics of DNA methylation fidelity during mouse embryonic stem cell self-renewal and differentiation (2014)

The faithful transmission of DNA methylation patterns through cell divisions is essential for the daughter cells to retain a proper cell identity. To achieve a comprehensive assessment of methylation fidelity, we implemented a genome-scale hairpin bisulfite sequencing approach to generate methylation data for DNA double strands simultaneously. We show here that methylation fidelity increases globally during differentiation of mouse embryonic stem cells (mESCs), and is particularly high in the promoter regions of actively expressed genes and positively correlated with active histone modification marks and binding of transcription factors. The majority of intermediately (40%-60%) methylated CpG dinucleotides are hemi-methylated and have low methylation fidelity, particularly in the differentiating mESCs. While 5-hmC and 5-mC tend to coexist, there is no significant correlation between 5-hmC levels and methylation fidelity. Our findings may shed new light on our understanding of the origins of methylation variations and the mechanisms underlying DNA methylation transmission.
Antihypertensive effects of the essential oil of <i>Alpinia zerumbet</i> and its main constituent, terpinen‐4‐ol, in DOCA‐salt hypertensive conscious rats (2003)

The present study investigated the hypotensive responses to intravenous (i.v.) treatment with the essential oil of Alpinia zerumbet (EOAZ) and its main constituent, terpinen-4-ol (Trp-4-ol), in the experimental model of deoxycorticosterone-acetate (DOCA)-salt hypertensive rat. In both DOCA-salt hypertensive and uninephrectomized, normotensive rats, i.v. bolus injections of EOAZ (1-20 mg/kg) or Trp-4-ol (1-10 mg/kg) decreased mean aortic pressure (MAP) in a dose-related manner. However, hypotensive responses to Trp-4-ol were significantly greater than those evoked by the same doses of EOAZ (1-10 mg/kg). Treatment with DOCA-salt significantly enhanced the maximal percentage decreases in MAP evoked by EOAZ or Trp-4-ol. Likewise, both maximal percentage and absolute decreases in MAP elicited by i.v. injection of the ganglion blocker, hexamethonium (30 mg/kg), were significantly greater in DOCA-salt hypertensive than in control rats. In DOCA-salt hypertensive rats, neither hexamethonium (30 mg/kg, i.v.) nor methylatropine (1 mg/kg, i.v.) pretreatment affected the enhancement of EOAZ-induced hypotension. These results show that i.v. treatment with either EOAZ or Trp-4-ol dose-dependently decreases blood pressure in conscious DOCA-salt hypertensive rats, and this action is enhanced when compared with uninephrectomized controls. This enhancement could be related mainly to an increase in EOAZ-induced vascular smooth muscle relaxation rather than to enhanced sympathetic nervous system activity in this hypertensive model. The data further support our previous hypothesis that hypotensive effects of EOAZ are partially attributed to the actions of Trp-4-ol.