gebhart/patrepeval huggingface dataset:
The<i>Pseudomonas aeruginosa</i>Autoinducer<i>N</i>-3-Oxododecanoyl Homoserine Lactone Accelerates Apoptosis in Macrophages and Neutrophils (2003)

Quorum-sensing systems are critical regulators of the expression of virulence factors of various organisms, including Pseudomonas aeruginosa. Las and Rhl are two major quorum-sensing components, and they are regulated by their corresponding autoinducers, N-3-oxododecanoyl homoserine lactone (3-oxo-C(12)-HSL) and N-butyryl-L-homoserine lactone (C(4)-HSL). Recent progress has demonstrated the potential of quorum-sensing molecules, especially 3-oxo-C(12)-HSL, for modulation of the host immune system. Here we show the specific ability of 3-oxo-C(12)-HSL to induce apoptosis in certain types of cells. When bone marrow-derived macrophages were incubated with synthetic 3-oxo-C(12)-HSL, but when they were incubated not C(4)-HSL, significant loss of viability was observed in a concentration (12 to 50 micro M)- and incubation time (1 to 24 h)-dependent manner. The cytotoxic activity of 3-oxo-C(12)-HSL was also observed in neutrophils and monocytic cell lines U-937 and P388D1 but not in epithelial cell lines CCL-185 and HEp-2. Cells treated with 3-oxo-C(12)-HSL revealed morphological alterations indicative of apoptosis. Acceleration of apoptosis in 3-oxo-C(12)-HSL-treated cells was confirmed by multiple criteria (caspases 3 and 8, histone-associated DNA fragments, phosphatidylserine expression). Structure-activity correlation experiments demonstrated that the fine structure of 3-oxo-C(12)-HSL, the HSL backbone, and side chain length are required for maximal activity. These data suggest that Pseudomonas 3-oxo-C(12)-HSL specifically promotes induction of apoptosis, which may be associated with 3-oxo-C(12)-HSL-induced cytotoxicity in macrophages and neutrophils. Our data suggest that the quorum-sensing molecule 3-oxo-C(12)-HSL has critical roles in the pathogenesis of P. aeruginosa infection, not only in the induction of bacterial virulence factors but also in the modulation of host responses.
Monolithic TV IF System TBA 440 (1972)

TBA 440 resembles a sensitive IF amplifier, a double balanced detector and an AGC system for IF and RF with adjustable threshold and tuner delay in a comon 16 lead DIP-case. The IF-amplifier is dc-coupled from input to the video outputs and gain controlled in two stages by variable emitter-degeneration. Thus the number of external components could be minimized. The circuit offers excellent large signal capability and low noise level over the whole AGC-range. The dc-level of the video outputs and of the AGC threshold are temperature compensated.
Validation of the UCLA Integrated Staging System for Patients With Renal Cell Carcinoma (2001)

Article Tools SPECIAL DEPARTMENTS Article Tools OPTIONS & TOOLS Export Citation Track Citation Add To Favorites Rights & Permissions COMPANION ARTICLES No companion articles ARTICLE CITATION DOI: 10.1200/JCO.2001.19.17.3792 Journal of Clinical Oncology - published online before print September 21, 2016 PMID: 11533107 Validation of the UCLA Integrated Staging System for Patients With Renal Cell Carcinoma Amnon ZismanxAmnon ZismanSearch for articles by this author , Allan J. PantuckxAllan J. PantuckSearch for articles by this author , Robert A. FiglinxRobert A. FiglinSearch for articles by this author , Arie S. BelldegrunxArie S. BelldegrunSearch for articles by this author Show More UCLA School of Medicine, Los Angeles, CA https://doi.org/10.1200/JCO.2001.19.17.3792 First Page Full Text PDF Figures and Tables © 2001 by American Society of Clinical Oncology
The central role of platelet activation in determining the severity of acute coronary syndromes: Table 1 (2003)

ecent observational studies in acute coronary syndromes have shown that factors influencing the degree of platelet activation also influence the mode of presentation. Prior aspirin treatment and smoking-with directionally opposite effects on platelet function-were found to be independently associated with unstable angina and myocardial infarction, respectively. 1 However, the few studies involving direct measurement of activation parameters in acute coronary syndromes have not provided a consensus regarding this. 2 3 To clarify whether there is differential activation in unstable angina and acute myocardial infarction we have prospectively assessed platelet activation in a cohort of patients presenting with acute coronary syndromes by using a light scattering technology to determine mean platelet component, a marker of platelet degranulation.
Racial Transgression (1911)

JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive.We use information technology and tools to increase productivity and facilitate new forms of scholarship.
A VLSI robotics vector processor for real-time control (2003)

A VLSI robotics vector processor (RVP) for real-time control is described. Hardware parallelism and pipelining is used to exploit potential concurrency in the low-level matrix/vector operations characteristic of the kinematics and dynamics computations required for control. Three floating point processors (FPP), each with a adder, multiplier, and register file, all operating in parallel in an SIMD (single-instruction, multiple data stream) fashion are incorporated. Data exchange between the FPPs is facilitated by a dual shift-broadcast network. High-speed dual I/O channels are provided so that input/output bottlenecks are avoided. The RVP uses a RISC (reduced-instruction-set-computer) architecture with seven basic instructions. Composite vector operations such as matrix-vector multiply and vector cross product may be readily programmed using the basic instruction set to given considerable overlap. The RVP can be implemented on a single VLSI chip using 1.2- mu m CMOS.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Effect of the compression of the depth map image on depth-fused 3D image quality (2003)

A depth-fused 3-D (DFD) display, which is composed of two 2-D images displayed at different depths, is a new 3-D display proposed recently and enables an observer using no extra equipment to perceive an apparent 3-D image. The original data for it are a 2-D image of objects and a depth map image of objects. The two 2-D images are formed by dividing the luminance of an original 2-D image between the two 2-D images according to a depth data of objects at each pixel. This paper presents the effect of the compression of the depth map image on a DFD image. We studied on still pictures using JPEG as an algorithm for compression. After decoding the depth map image, 3-D images were displayed forming the two 2-D images. The main result obtained from subjective evaluations is that the effect of the compression noises appearing on its decoded image appears as errors of position in depth on DFD image, however, a higher compression rate is possible for depth map image than for conventional 2-D image. This result shows that is is advantageous to transmit or store the original data before forming the two 2-D images.
Nanotopography as a trigger for the microscale, autogenous and passive lysis of erythrocytes (2014)

Microscale devices are increasingly being developed for diagnostic analysis although conventional lysis as an initial step presents limitations due to its scale or complexity. Here, we detail the physical response of erythrocytes to the surface nanoarchitecture of black Si (bSi) and foreshadow their potential in microanalysis. The physical interaction brought about by the spatial convergence of the two topologies: (a) the nanopillar array present on the bSi and (b) the erythrocyte cytoskeleton present on the red blood cells (RBCs), provides spontaneous stress-induced cell deformation, rupture and passive lysis within an elapsed time of ∼3 min from immobilisation to rupture and without external chemical or mechanical intervention. The mechano-responsive bSi surface provides highly active yet autogenous RBC lysis and a prospect as a front-end platform technology in evolving micro-fluidic platforms for cellular analyses.
Enrichment of Bovine X-and Y-Chromosome-Bearing Sperm with Monoclonal H-Y Antibody-Fluorescence-Activated Cell Sorter (1990)

Bovine spermatozoa were assessed indirectly for the presence of a Y chromosome by monitoring expression of the H-Y antigen. Spermatozoa labeled with a monoclonal H-Y antibody (MoAb) and fluorescein-conjugated goat antibody to mouse F(ab)2 were counted with both a fluorescent microscope and a fluorescence-activated cell sorter (FACS). Of ejaculated spermatozoa, 40% to 60% fluoresced by this procedure compared to 1% to 15% of sperm reacted with nonimmune serum. Semen from three bulls was exposed to nonimmune serum (control) or MoAb, sorted by FACS, and analyzed for DNA content with a scanning microdensitometer. Control samples showed two distinct peaks with a mean difference in DNA content of 3.95%; these peaks were assumed to represent Y- and X-chromosome-bearing spermatozoa populations, respectively. DNA analyses of the MoAb-treated sperm of three bulls that sorted positively for H-Y antigen (fluorescent sperm) yielded ratios of Y- to X-chromosome-bearing spermatozoa of 76 : 24, 88 : 12, and 77 : 23, and those sorted negatively for H-Y antigen (nonfluorescent sperm) yielded ratios of 26 : 74, 35 : 65, and 23 : 77. The proportions of Y- and X-chromosome-bearing spermatozoa in nonsorted samples were not different from 50 : 50. Suitable MoAbs can be used in conjunction with FACS to enrich the proportion of Y- or X-chromosome-bearing spermatozoa in bovine semen.
A Series of 6- and 7-Piperazinyl- and -Piperidinylmethylbenzoxazinones with Dopamine D4 Antagonist Activity:  Discovery of a Potential Atypical Antipsychotic Agent (1999)

As part of a program to develop dopamine D4 antagonists for the treatment of schizophrenia, we discovered a series of 6- and 7-(phenylpiperazinyl)- and -(phenylpiperidinyl)methylbenzoxazinones through mass screening of our compound library. A structure−activity relationship SAR study was carried out involving substituents on the phenyl ring, and several selective D4 antagonists were identified. The 7-substituted benzoxazinones showed more activity in neurochemical and behavioral tests than the 6-substituted series. One of the most potent and selective compounds (26) was found to have potent activity in animal tests predictive of antipsychotic activity in humans after oral administration. This paper describes the SAR of the benzoxazinone series and the preclinical characterization of 26.
<i>Bordetella</i> PlrSR regulatory system controls BvgAS activity and virulence in the lower respiratory tract (2017)

Bacterial pathogens coordinate virulence using two-component regulatory systems (TCS). The <i>Bordetella</i> virulence gene (BvgAS) phosphorelay-type TCS controls expression of all known protein virulence factor-encoding genes and is considered the "master virulence regulator" in <i>Bordetella pertussis</i>, the causal agent of pertussis, and related organisms, including the broad host range pathogen <i>Bordetella bronchiseptica</i> We recently discovered an additional sensor kinase, PlrS [for persistence in the lower respiratory tract (LRT) sensor], which is required for <i>B. bronchiseptica</i> persistence in the LRT. Here, we show that PlrS is required for BvgAS to become and remain fully active in mouse lungs but not the nasal cavity, demonstrating that PlrS coordinates virulence specifically in the LRT. PlrS is required for LRT persistence even when BvgAS is rendered constitutively active, suggesting the presence of BvgAS-independent, PlrS-dependent virulence factors that are critical for bacterial survival in the LRT. We show that PlrS is also required for persistence of the human pathogen <i>B. pertussis</i> in the murine LRT and we provide evidence that PlrS most likely functions via the putative cognate response regulator PlrR. These data support a model in which PlrS senses conditions present in the LRT and activates PlrR, which controls expression of genes required for the maintenance of BvgAS activity and for essential BvgAS-independent functions. In addition to providing a major advance in our understanding of virulence regulation in <i>Bordetella</i>, which has served as a paradigm for several decades, these results indicate the existence of previously unknown virulence factors that may serve as new vaccine components and therapeutic or diagnostic targets.
A NEW TRAFFIC MODEL FOR CURRENT USER WEB BROWSING BEHAVIOR (2007)

Given the wide use of HTTP traffic models to model user web browsing behaviour, it is important that the model be representative of a large variety of traffic and be continually updated to reflect the constantly evolving nature of web content and the exponential growth in number of users. In this paper, we analyzed an extensive set of proxy web server logs to understand changes in network traffic patterns. We found significant gaps in the methods previously proposed, specifically the major one being that it is almost impossible to detect a web request generated from a user click from one generated from various embedded scripts and frames. As a result, we modified the definition of a web request boundary. Due to the presence of large numbers of embedded objects from several different off-site sources, which cannot be traced back to the original request through following TCP/IP headers source addresses alone, newer heuristics need to be devised. We present our methodology for analyzing the squid proxy log in a way that preserves user privacy, and propose a new HTTP traffic model and traffic generator to represent current user web browsing behaviour. Comparison of independent statistics from the trace and the model shows a fair match.
3D imaging of myocardial perfusion and coronary tree morphology from a single rotational angiogram (2011)

Diagnosis and treatment of coronary heart disease are performed in the catheter laboratory using an angiographic X-ray C-arm system. The morphology of the coronary tree and potentially ischemic lesions are determined in 2D projection views. The hemodynamic impact of the lesion would be valuable information for treatment decision. Using other modalities for functional imaging is disrupting the clinical workflow since the patient has to be transferred from the catheter laboratory to another scanner, and back to the catheter laboratory for performing the treatment. In this work a novel technology is used for simultaneous 3D imaging of first pass perfusion and the morphology of the coronary tree from a single rotational angiogram. A selective, single shot of contrast agent of less than 20ml directly into the coronaries is sufficient for a proper contrast resolution. Due to the long acquisition time cardiac motion has to be considered. A novel reconstruction technique for estimation and compensation of cardiac motion from the acquired projection data is used. The overlay of the 3D structure of the coronary tree and the perfusion image shows the correlation of myocardial areas and the associated coronary sections supporting that region. In a case example scar lesions caused by a former myocardial infarct are investigated. A first pass perfusion defect is found which is validated by a late enhancement magnetic resonance image. No ischemic defects are found. The non vital regions are still supported by the coronary vasculature.
Coupled exposure to ingredients of cosmetic products: <scp>III</scp>. Ultraviolet filters (2014)

The frequent co-occurrence of UV filters in cosmetic products possibly facilitates sensitization, and may explain why patients often react to chemically unrelated UV filters.
Switchable 3D liquid crystal grating generated by periodic photo-alignment on both substrates (2015)

A planar liquid crystal (LC) cell is developed in which two photo-alignment layers have been illuminated with respectively a horizontal and a vertical diffraction pattern of interfering left- and right-handed circularly polarized light. In the bulk of the cell, a complex LC configuration is obtained with periodicity in two dimensions. Remarkably, the period of the structure is larger than the period of the interference pattern, indicating that lowering of the symmetry allows a reduction in the elastic energy. The liquid crystal configuration depends on the periodicity of the alignment but also on the thickness of the cell. By applying a voltage over the electrodes, the power going into the different diffracted orders can be tuned. Finite element (FE) simulations based on Q-tensor theory are used to find the 3D equilibrium director distribution, which is used to simulate the near-field transmission profile based on the Jones calculus. A 2D Fourier transform is performed for both the x- and y-component of the transmitted wave to find the diffraction efficiency.
Sarcoidosis and spondylarthropathy. Three case-reports. (1996)

Coexistent sarcoidosis and seronegative spondylarthropathy have rarely been reported. We add three new cases to the nine previously published. Two men and one woman with sarcoidosis met Amor's criteria for spondylarthropathy. The diagnosis of sarcoidosis was based on histologic findings in two cases and on roentgenographic and laboratory test findings in one case. The features of each of the two diseases were unremarkable. The two diagnoses were confirmed at about the same time. Osteoarticular manifestations of sarcoidosis are reviewed. Our case-reports illustrate the diagnostic difficulties raised by discovery of sacroiliitis in a patient with sarcoidosis: sarcoid osteitis, infection, or a spondyloarthropathy can be the cause of the sacroiliac lesions. Moreover, the pelvic and spinal manifestations of sarcoidosis can mimick a spondylarthropathy. Coexistence of sarcoidosis and spondylarthropathy is probably due to chance, since there are no shared predisposing genetic factors and the number of reported cases is small.
Characterization of different promoters for designing a new expression vector in <i>Saccharomyces cerevisiae</i> (2010)

The widely used pESC vector series (Stratagene, La Jolla, CA, USA) with the bidirectional GAL1/GAL10 promoter provides the possibility of simultaneously expressing two different genes from a single vector in Saccharomyces cerevisiae. This system can be induced by galactose and is repressed by glucose. Since S. cerevisiae prefers glucose as a carbon source, and since its growth rate is higher in glucose than in galactose-containing media, we compared and evaluated seven different promoters expressed during growth on glucose (pTEF1, pADH1, pTPI1, pHXT7, pTDH3, pPGK1 and pPYK1) with two strong galactose-induced promoters (pGAL1 and pGAL10), using lacZ as a reporter gene and measuring LacZ activity in batch and continuous cultivation. TEF1 and PGK1 promoters showed the most constant activity pattern at different glucose concentrations. Based on these results, we designed and constructed two new expression vectors which contain the two constitutive promoters, TEF1 and PGK1, in opposite orientation to each other. These new vectors retain all the features from the pESC-URA plasmid except that gene expression is mediated by constitutive promoters.
Myocardial perfusion imaging protocols: is there an ideal protocol? (2007)

Is there an ideal myocardial perfusion imaging protocol? In order to answer this question and choose a protocol for clinical use, one must understand the characteristics of the available radiopharmaceuticals, the protocol variables, and the advantages and disadvantages of each. After reading this article, the technologist should be able to list the ideal characteristics of a myocardial perfusion imaging agent, describe and compare the characteristics of myocardial perfusion imaging agents, discuss the relationship between coronary blood flow and myocardial uptake of various tracers, describe imaging protocols, and discuss the advantages and disadvantages of each protocol.
Bacterial Expression of the Catalytic Domain of 3–Hydroxy‐3–Methylglutaryl‐CoA Reductase (Isoform HMGR1) from <i>Arabidopsis thaliana</i>, and Its Inactivation by Phosphorylation at Ser577 by <i>Brassica oleracea</i> 3‐Hydroxy‐3‐Methylglutaryl‐CoA Reductase Kinase (1995)

The catalytic domain of 3-hydroxy-3-methylglutaryl-CoA reductase isoform 1 (HMGR1cd) from Arabidopsis thaliana has been expressed in Escherichia coli in a catalytically active form and purified. The high efficiency of the bacterial expression system together with the simplicity of the purification procedure used in this study resulted in the attainment of large quantities of pure enzyme (about 5 mg/l culture) with a final specific activity of up to 17 U/mg. This specific activity is higher than that reported to date for any 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) purified from a plant source. HMGR1cd activity was completely blocked by the HMGR inhibitor mevinolin (IC50 = 12.5 nM). No significant differences were observed between the Km values of HMGR1cd for NADPH (71 +/- 7 microM) and (S)-3-hydroxy-3-methylglutaryl-CoA (8.3 +/- 1.5 microM) and those of pure HMGR preparations obtained from different plant sources. The purified HMGR1cd was reversibly inactivated by phosphorylation at a single site by Brassica oleracea HMGR kinase A, which is functionally related to the mammalian AMP-activated protein kinase. The site of phosphorylation is Ser577 in the complete sequence of A. thaliana HMGR1. The results in this paper represent the first evidence that a higher plant HMGR is regulated by direct phosphorylation, at least in a cell-free system. Our results also reinforce the view that the AMP-activated protein kinase/SNF1 family is an ancient and highly conserved protein kinase system.
Monodispersed, ultrathin NiPt hollow nanospheres with tunable diameter and composition via a green chemical synthesis (2014)

Monodisperse NiPt hollow nanospheres with tunable sizes were synthesized by a green chemical synthesis, which have excellent electrocatalytic properties.
Fast search algorithms for vector quantization of images using multiple triangle inequalities and wavelet transform (2000)

The encoding of vector quantization (VQ) needs expensive computation for searching the closest codevector to the input vector. This paper presents several fast encoding algorithms based on multiple triangle inequalities and wavelet transform to overcome this problem. The multiple triangle inequalities confine a search range using the intersection of search areas generated from several control vectors. A systematic way for designing the control vectors is also presented. The wavelet transform combined with the partial distance elimination is used to reduce the computational complexity of the distance calculation of vectors. The proposed algorithms provide the same coding quality as the full search method. The experimental results indicate that the new algorithms perform more efficiently than existing algorithms.
Aggression classification and treatment response. (1998)

This preliminary study investigated whether the aggression subtypes derived from the Aggression Questionnaire (AQ) are related to treatment response. The subjects were 28 aggressive conduct-disordered children (25 males, 3 females), ranging in age from 9.8 to 17.0 years (mean age = 12.69 years), who participated in a double-blind, placebo-controlled study of lithium as a treatment for reducing aggression. We used the Predatory-Affective Index of the AQ to classify subjects into "predatory" (planned) or "affective" (explosive) subtypes of aggression and then related this classification to treatment response. This index did not differentiate placebo baseline responders from nonresponders. However, the Index did significantly differentiate responders and nonresponders during the experimental treatment period, regardless of whether they received lithium or placebo. Treatment response was associated with a more affective and less predatory subtype of aggression. To the best of our knowledge, this is the first study in children to show an association between the aggression subtype and treatment response.