gebhart/patrepeval huggingface dataset:
Inhibition of Inositol Phosphorylceramide Synthase by the Cyclic Peptide Aureobasidin A (2008)

By using a detergent-washed membrane preparation, the interaction of the fungal natural product inhibitor aureobasidin A (AbA) with inositol phosphorylceramide synthase (IPC synthase) was studied by kinetic analysis of wild-type and mutant enzyme-catalyzed reactions. AbA inhibited the wild-type enzyme from both Candida albicans and Saccharomyces cerevisiae in an irreversible, time-dependent manner, with apparent K(i) values of 183 and 234 pM, respectively. Three synthetic chemistry-derived AbA derivatives, PHA-533179, PHA-556655, and PHA-556656, had affinities 4 to 5 orders of magnitude lower and were reversible inhibitors that competed with the donor substrate phosphatidylinositol (PI). AbA was a reversible, apparently noncompetitive inhibitor, with a K(i) of 1.4 microM, of the IPC synthase from an AbA-resistant S. cerevisiae mutant. The K(m) values for both substrates (ceramide and PI) were similar when they interacted with the mutant and the wild-type enzymes. By contrast, the V(max) for the mutant enzyme was less than 10% of that for the wild-type enzyme. A comparison of the results obtained with AbA with those obtained with two other natural products inhibitors, rustmicin and khafrefungin, revealed that while rustmicin appeared to be a reversible, noncompetitive inhibitor of the wild-type enzyme, with a K(i) of 16.0 nM, khafrefungin had the kinetic properties of a time-dependent inhibitor and an apparent K(i) of 0.43 nM. An evaluation of the efficiencies of these compounds as inhibitors of the mutant enzyme revealed for both a drop in the apparent affinity for the enzyme of more than 2 orders of magnitude.
Selected Information Retrieval Abstracts (1990)

article Free Access Share on Selected Information Retrieval Abstracts Reviewer: Gerry Salton View Profile Authors Info & Claims ACM SIGIR ForumVolume 24Issue 3Fall 1990 pp 76–85https://doi.org/10.1145/101306.1096782Published:01 November 1990Publication History 0citation157DownloadsMetricsTotal Citations0Total Downloads157Last 12 Months0Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my Alerts New Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteeReaderPDF
Composition and Structure of the Martian Atmosphere: Preliminary Results from Viking 1 (1976)

Results from the aeroshell-mounted neutral mass spectrometer on Viking I indicate that the upper atmosphere of Mars is composed mainly of CO(2) with trace quantities of N(2), Ar, O, O(2), and CO. The mixing ratios by volume relative to CO(2) for N(2), Ar, and O(2) are about 0.06, 0.015, and 0.003, respectively, at an altitude near 135 kilometers. Molecular oxygen (O(2)(+)) is a major component of the ionosphere according to results from the retarding potential analyzer. The atmosphere between 140 and 200 kilometers has an average temperature of about 180 degrees +/- 20 degrees K. Atmospheric pressure at the landing site for Viking 1 was 7.3 millibars at an air temperature of 241 degrees K. The descent data are consistent with the view that CO(2) should be the major constituent of the lower martian atmosphere.
Detroit: Contracting for Quality (1993)

The American automotive industry is a pyramid of responsibility. The manufacturers do just one thing: they build vehicles. The tools of automation which make this possible are developed and installed by a large population of independent contractors. These shops in turn rest on a flexible base of workers with a broad range of skills. In the final analysis, it always comes down to people.
The Effect of Secretory Factors of Adipose-Derived Stem Cells on Human Keratinocytes (2012)

AAPE activates HK proliferation and migration. These results highlight the potential of the topical application of AAPE in the treatment of skin regeneration.
Multi-Q:  A Fully Automated Tool for Multiplexed Protein Quantitation (2006)

The iTRAQ labeling method combined with shotgun proteomic techniques represents a new dimension in multiplexed quantitation for relative protein expression measurement in different cell states. To expedite the analysis of vast amounts of spectral data, we present a fully automated software package, called Multi-Q, for multiplexed iTRAQ-based quantitation in protein profiling. Multi-Q is designed as a generic platform that can accommodate various input data formats from search engines and mass spectrometer manufacturers. To calculate peptide ratios, the software automatically processes iTRAQ's signature peaks, including peak detection, background subtraction, isotope correction, and normalization to remove systematic errors. Furthermore, Multi-Q allows users to define their own data-filtering thresholds based on semiempirical values or statistical models so that the computed results of fold changes in peptide ratios are statistically significant. This feature facilitates the use of Multi-Q with various instrument types with different dynamic ranges, which is an important aspect of iTRAQ analysis. The performance of Multi-Q is evaluated with a mixture of 10 standard proteins and human Jurkat T cells. The results are consistent with expected protein ratios and thus demonstrate the high accuracy, full automation, and high-throughput capability of Multi-Q as a large-scale quantitation proteomics tool. These features allow rapid interpretation of output from large proteomic datasets without the need for manual validation. Executable Multi-Q files are available on Windows platform at http://ms.iis.sinica.edu.tw/Multi-Q/.
Dynamo: a model for a distributed multi-media information processing environment (2003)

Dynamo is an object-oriented information model that underlies the Dynamo system, a general-purpose system for information management in a shared, distributed environment. The aim of the Dynamo system is to serve as a software platform for supporting a broad range of multimedia information processing activities than can be undertaken on a technical workstation. The Dynamo model synthesizes concepts from object-oriented programming languages and databases, hypermedia, CAD, (computer-aided design), and simulation. The core of the model consists of a very simple notion of active objects of which virtual time is an integral element. Objects are defined incrementally using cloning and delegation, and realize hypertextlike nodes and links with more expressive power than the usual passive links of hypertext. Virtual time provides the basis for a uniform approach to synchronization, versioning, and configuration control.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Coagulation Disorders and Tumor Markers in the Diagnosis of Pancreatic Cancer (1991)

Twenty patients (42-80 years old of whom 9 women) affected by instrumentally ascertained pancreatic cancer (7 cases were operated on) were studied. In all of them the following coagulation indices (fibrinopeptide A, FpA; beta-thromboglobulin, BTG; platelet factor IV, PF4; fibrinogen degradation products, XDP) and tumor markers (gastrointestinal cancer associated antigen, GICA; tissue polypeptide antigen, TPA; carcinoembryonic antigen, CEA; alpha-fetoprotein, or AFP) were assessed at the time of diagnosis, and 10 and 30 days after diagnosis, to test whether and which of the above parameters are more sensitive for entertaining the underlying affection. In both operated and nonoperated patients FpA was shown to be the most sensitive index. Lesser sensitivity was shown by XDP, GICA, and BTG. AFP proved to be quite useless as its serum levels constantly fell within the normal range.
Abnormal metabolic network activity in REM sleep behavior disorder (2014)

Latent network abnormalities in subjects with idiopathic RBD are associated with a greater likelihood of subsequent phenoconversion to a progressive neurodegenerative syndrome.
Calibrating Growth in the Ambitious SME: Growth Mapping as a New Direction in Business Model Visualisation (2013)

Research relating to management assumptions1 has largely being confined to mature industries where they are readily recognisable as influential business variables in finance, economics, organisational and business development. These studies have primarily considered senior managerial assumptions as part of the cognitive architecture of the firm, driving many developmental and growth processes of the organisation. This perspective of assumptions acting as intellectual assets of the organisation supporting the firm and its development is however largely absent from entrepreneurial and small business research. This is somewhat surprising given that research on the small firm suggests that entrepreneurs tend to operate on a high assumption to knowledge ratio. This paper, which introduces assumptive-led ‘growth maps’ attempts to address this apparent deficiency and considers how entrepreneurial assumptions impact upon the planning and development of ambitious, owner-managed, indigenous, Irish small and medium enterprises (SMEs).
G2 repair and evaluation of the cytogenetic damage induced by low doses of X-irradiation during G0 in human lymphocytes. (1995)

In the present study two cytogenetic parameters were used to evaluate the DNA damage induced by low doses (1 up to 40 rad) of X-ray irradiation in G0 human lymphocytes. These parameters were the frequency of chromosomal lesions in G2 and the length of this cell cycle phase. The frequency of chromosomal lesions in G2 was determined by scoring the number of chromosomal aberrations in G0 irradiated lymphocytes post treated with two inhibitors of G2 repair mechanisms: caffeine and 3-aminobenzamide. A dose-dependent increase in chromosomal aberrations yield was detected in G0 lymphocytes X-ray irradiated with or without post treatment with these two DNA repair inhibitors during G2. Nevertheless, the dose response in this latter condition was higher than the one detected in control cells, indicating that the increase of irradiation dose in G0 lymphocytes produces an increment in the number of DNA lesions arriving to be repaired in G2. The analysis of the dose-response relationships for G2 length showed an statistically significant X-ray dose-dependent increase (G2 delay) from 2.5 up to 40 rad and a positive correlation between G2 delay and the frequency of chromosomal lesions in G2. These results suggest that the DNA lesions induced by low doses of X-irradiation in G0 lymphocytes may be higher than that detected by the standard method (control conditions) and may be responsible for an increase in G2 length. We propose, therefore, that an analysis of these two cytogenetic parameters can improve the evaluation of the DNA damage induced by low doses of X-rays irradiation in G0 cells.
A Jig for Pin Insertion in the Performance of High Tibial Osteotomy (1975)

The crucial step in high tibial osteotomy is to translate the computed angle of correction into a bone wedge outlined by 2 Kisrchner wires to guide the surgery. The wedge can be excised with precision in 2 planes. A jig facilitates accurate placement of the pins to correspond exactly to the predetermined angles.
Complete amino acid sequence of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. (1988)

The complete amino acid sequence of 6-phospho-fructo-2-kinase/fructose-2,6-bisphosphatase from rat liver was determined by direct analysis of the S-carboxamidomethyl protein. A complete set of nonoverlapping peptides was produced by cleavage with a combination of cyanogen bromide and specific proteolytic enzymes. The active enzyme is a dimer of two identical polypeptide chains composed of 470 amino acids each. The NH2-terminal amino acid residue of the polypeptide chain was shown to be N-acetylserine by fast atom bombardment mass spectrometry of the purified N-terminal tetradecapeptide isolated after cleavage of the intact S-carboxamidomethylated protein with lysyl endoproteinase (Achromobacter protease I). Alignment of the set of unique peptides was accomplished by the analysis of selected overlapping peptides generated by proteolytic cleavage of the intact protein and the larger purified cyanogen bromide peptides with trypsin, Staphylococcus aureus V8 protease, and lysyl endoproteinase. Four nonoverlapping peptides were aligned by comparison with the amino acid sequence predicted from a partial cDNA clone encoding amino acid positions 166-470 of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (Colosia, A.D., Lively, M., El-Maghrabi, M. R., and Pilkis, S. J. (1987) Biochem. Biophys. Res. Commun. 143, 1092-1098). The nucleotide sequence of the cDNA corroborated the peptide sequence determined by direct methods. A search of the Protein Identification Resource protein sequence database revealed that the overall amino acid sequence appears to be unique since no obviously homologous sequences were identified. However, a 100-residue segment of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (residues 250-349), including the active site histidine residue of the bisphosphatase domain, was found to be homologous to the active site regions of yeast phosphoglycerate mutase and human bisphosphoglycerate mutase.
A Study of Geothermal Heat Pump and Standing Column Well Performance (2004)

Standing column wells can be used as highly efficient ground heat exchangers in geothermal heat pump systems, where hydrological and geological conditions are suitable. A numerical model of groundwater flow and heat transfer in and around standing column wells has been developed. This model has been used in a parametric study to identify the most significant design parameters and their effect on well performance. For each case in the study, performance has been evaluated in terms of minimum and maximum annual temperatures and design well depth. Energy consumption and annual costs have also been calculated. Groundwater “bled” from the system is one of the most significant parameters a system designer can use to improve well performance for a given load. The effects of bleed rate, well depth, and rock properties on heat transfer and energy consumption are discussed.
Learning SQL (2005)

SQL (Structured Query Language) is a standard programming language for generating, manipulating, and retrieving information from a relational database. If you're working with a relational database--whether you're writing applications, performing administrative tasks, or generating reports--you need to know how to interact with your data. Even if you are using a tool that generates SQL for you, such as a reporting tool, there may still be cases where you need to bypass the automatic generation feature and write your own SQL statements. To help you attain this fundamental SQL knowledge, look to Learning SQL, an introductory guide to SQL, designed primarily for developers just cutting their teeth on the language. Learning SQL moves you quickly through the basics and then on to some of the more commonly used advanced features. Among the topics discussed: The history of the computerized database SQL Data Statements--those used to create, manipulate, and retrieve data stored in your database; example statements include select, update, insert, and delete SQL Schema Statements--those used to create database objects, such as tables, indexes, and constraints How data sets can interact with queries The importance of subqueries Data conversion and manipulation via SQL's built-in functions How conditional logic can be used in Data Statements Best of all, Learning SQL talks to you in a real-world manner, discussing various platform differences that you're likely to encounter and offering a series of chapter exercises that walk you through the learning process. Whenever possible, the book sticks to the features included in the ANSI SQL standards. This means you'll be able to apply what you learn to any of several different databases; the book covers MySQL, Microsoft SQL Server, and Oracle Database, but the features and syntax should apply just as well (perhaps with some tweaking) to IBM DB2, Sybase Adaptive Server, and PostgreSQL. Put the power and flexibility of SQL to work. With Learning SQL you can master this important skill and know that the SQL statements you write are indeed correct.
Single modulator joint transform correlator architectures (1989)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text Bahram Javidi, Don A. Gregory, and Joseph L. Horner, "Single modulator joint transform correlator architectures," Appl. Opt. 28, 411-413 (1989) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Über Antamanid, XVI. all‐D‐retro‐Antamanid und D‐Tyr<sup>6</sup>‐all‐D‐retro‐antamanid (1972)

Abstract Die im Titel genannten Verbindungen 2a und 2b werden aus den entsprechenden linearen, automatisch auf der Festphase synthetisierten Decapeptiden durch Cyclisierung mit Dicyclohexylcarbodiimid und N ‐Hydroxy‐succinimid hergestellt. 2a und 2b sind in Wasser so schwer löslich, daß ihre Schutzwirkung nicht untersucht werden kann, doch bewirkt der wasserlösliche Schwefelsäureester von 2b mit 2 mg pro kg völligen Schutz der weißen Maus vor Vergiftung mit 5 mg Phalloidin.
Free Energy Barriers to Evaporation of Water in Hydrophobic Confinement (2012)

We use umbrella sampling Monte Carlo and forward and reverse forward flux sampling (FFS) simulation techniques to compute the free energy barriers to evaporation of water confined between two hydrophobic surfaces separated by nanoscopic gaps, as a function of the gap width, at 1 bar and 298 K. The evaporation mechanism for small (1 × 1 nm(2)) surfaces is found to be fundamentally different from that for large (3 × 3 nm(2)) surfaces. In the latter case, the evaporation proceeds via the formation of a gap-spanning tubular cavity. The 1 × 1 nm(2) surfaces, in contrast, are too small to accommodate a stable vapor cavity. Accordingly, the associated free energy barriers correspond to the formation of a critical-sized cavity for sufficiently large confining surfaces, and to complete emptying of the gap region for small confining surfaces. The free energy barriers to evaporation were found to be of O(20kT) for 14 Å gaps, and to increase by approximately ~5kT with every 1 Å increase in the gap width. The entropy contribution to the free energy of evaporation was found to be independent of the gap width.
Use of Intelligent Techniques for Analysis of Faults and Protective Relay Operations (2007)

Analysis of faults and protective relay operations is crucial for understanding the fault clearing sequences. Today, this analysis is performed off line and mostly through manual inspection of different data records captured by digital protective relays (DPRs), digital fault recorders (DFRs), Sequence of Events Recorders (SERs), and other substation IEDs. This paper introduces several tools developed for automated analysis of faults and protective relay operations. The tools are implemented using intelligent techniques based on synchronized sampling, expert systems, neural networks, and fuzzy logic.
Phenotypic features of breast cancer cells overexpressing ornithine‐decarboxylase (1995)

Polyamines (PA) have been shown to be critical mediators of estradiol-induced breast cancer cell proliferation. This finding suggests that constitutive activation of the PA pathway may promote tumor progression, possibly leading to hormone independence. To test this hypothesis, we transfected hormone-responsive MCF-7 breast cancer cells with a complementary DNA coding for ornithine-decarboxylase (ODC), the first rate-limiting enzyme in PA biosynthesis. Marked ODC overexpression observed in stably transfected clones was associated with a selective increase in cellular putrescine content, while spermidine and spermine levels were not altered. ODC-overexpressing MCF-7 cells were resistant to the antiproliferative effects of low but not high concentrations of the enzyme inhibitor, alpha-difluoromethylornithine. In agreement with our hypothesis, sensitivity to the growth-promoting action of estradiol was reduced by approximately one third (P < 0.001) in ODC-overexpressing MCF-7 cells compared with vector-only transfected clones. Basal growth under anchorage-dependent conditions was only marginally increased by ODC overexpression (P = 0.048), while clonogenicity in soft agar was actually reduced. These data suggest that activation of PA biosynthesis may contribute in part to the acquisition of estrogen independence by breast cancer cells. Since only putrescine content was increased as a result of ODC overexpression, these data may underestimate the overall influence of the PA pathway on breast cancer phenotype.
Oxygen induced preferred orientation of dc sputtered platinum (1981)

Platinum thin films have been deposited by dc sputtering on glass substrates. The holding substrate temperature is maintained at 200 °C. The films are studied by x‐ray diffraction with a Bragg–Brentano diffractometer. The crystalline structure of platinum is faced centred cubic (fcc). In pure argon, the orientation is with the (111) plane parallel to the substrate. The orientation is found to change when oxygen is mixed with argon. Factors driving the orientation are the ratio of oxygen partial pressure relative to the deposition rate (R) and the thickness of the film (T). For a thickness larger or equal to 630 Å, the film orientation is (200) when R is around 3.3×10−4 Pa min Å−1, and (220) when R is around 4×10−3. When the films are thinner (270 Å for R?3.3×10−4 and 320 Å for R?4×10−4) the orientation is (111). A correlation is found between orientation and resistivity.
Extensive translation of circular RNAs driven by N6-methyladenosine (2017)

Extensive pre-mRNA back-splicing generates numerous circular RNAs (circRNAs) in human transcriptome. However, the biological functions of these circRNAs remain largely unclear. Here we report that N<sup>6</sup>-methyladenosine (m<sup>6</sup>A), the most abundant base modification of RNA, promotes efficient initiation of protein translation from circRNAs in human cells. We discover that consensus m<sup>6</sup>A motifs are enriched in circRNAs and a single m<sup>6</sup>A site is sufficient to drive translation initiation. This m<sup>6</sup>A-driven translation requires initiation factor eIF4G2 and m<sup>6</sup>A reader YTHDF3, and is enhanced by methyltransferase METTL3/14, inhibited by demethylase FTO, and upregulated upon heat shock. Further analyses through polysome profiling, computational prediction and mass spectrometry reveal that m<sup>6</sup>A-driven translation of circRNAs is widespread, with hundreds of endogenous circRNAs having translation potential. Our study expands the coding landscape of human transcriptome, and suggests a role of circRNA-derived proteins in cellular responses to environmental stress.
Switched capacitor bandgap voltage reference for sub-1-V operation (2006)

A switched capacitor bandgap voltage reference circuit capable of sub-1-V operation is presented. The proposed circuit generates a sub-1-V reference voltage with a switched capacitor operation, offering several performance advantages over the current-mode sub-1-V counterpart. The proposed design provides an improved supply rejection and outperforms in the presence of process variation. In addition, the circuit does not require a start-up circuit and the associating trimming circuitry is simplistic and cost effective. The proposed sub-1-V switched capacitor BGR can be a practical sub-1-V reference candidate in ultra deep sub-micron CMOS processes.