gebhart/patrepeval huggingface dataset:
Gene therapy: what have we accomplished and where do we go from here? (2005)

As a potential treatment for arthritis, gene transfer should be viewed within the context of biological therapy. Its particular strengths include the ability to deliver therapeutic gene products, both RNA and protein, to specific cells or tissues in a targeted, sustained, and potentially regulated, cost-effective fashion. An expanded definition of gene therapy includes the delivery of noncoding nucleotide sequences that act, for example, as decoy molecules. Considerable experimental progress has been made in the preclinical development of gene therapies for arthritis. Indeed, there is overwhelming proof of principle in animal models of rheumatoid arthritis (RA) and accumulating evidence of efficacy in animal models of osteoarthritis (OA). Early-phase human clinical trials have been successfully conducted and others are in progress. Additional research is necessary to optimize gene transfer technologies and achieve regulated transgene expression. However, the most urgent need is for interventional studies in human disease and the funding with which to implement them.
The code of codes: scientific and social issues in the human genome project (1993)

Reviewsmarriage certificate or birth certificates for their children, but from the rational explanation this equivocal
PRELIMINARY INVESTIGATION OF CALCIUM ALGINATE GEL AS A BIOCOMPATIBLE MATERIAL FOR ENDOVASCULAR ANEURYSM EMBOLIZATION IN VIVO (2007)

Calcium alginate was an effective endovascular occlusion material that filled the aneurysm and provided an effective template for tissue growth across the aneurysm neck after 30 days and up to 90 days. Complete filling of the aneurysm with calcium alginate ensures stability, biocompatibility, and optimal healing for up to 90 days in swine.
Spin Transport in Single- and Multilayer Graphene (2010)

Abstract not Available.
Molecular and functional analysis of the mariner mutator element Mos1 in Drosophila. (1991)

The white-peach allele in Drosophila results from insertion of the transposable element mariner. The particular copy that is inserted in white-peach is an inactive copy referred to as the peach element. The peach element is excised at a high rate in the presence of active copies of mariner located elsewhere in the genome, and the excision of peach in somatic cells is recognized phenotypically by the occurrence of eye-color mosaicism in white-peach flies. Active mariner elements identified by their ability to induce high levels of white-peach mosaicism are denoted Mos (Mosaic) factors. We have sequenced and functionally analyzed the factor Mos1 originally identified in Drosophila mauritiana. The Mos1 element is 1286 base pairs in length, the same length as the peach element. It differs from the peach element in 11 nucleotide positions distributed throughout its length, including four amino acid replacements in the long open reading frame. Analysis of chimeric constructs between Mos1 and peach implies that functionally important differences occur in both the 5' and 3' halves of Mos1. A mariner element identical in sequence to Mos1 yields lower levels of mosaicism in transformants, implying that adjacent flanking sequences have important effects on Mos1 activity. Another mariner element, designated Ma351, isolated from a nonmosaic strain of D. mauritiana, differs from Mos1 in just three nucleotide positions. When introduced into the germline, Ma351 yields various levels of white-peach mosaicism depending on insertion site. These results imply that the activity of mariner elements is determined jointly by their own nucleotide sequences, by the effects of adjacent flanking sequences, and by longer-range position effects.
Palladium-Catalyzed Enantioselective Synthesis of Carbanucleosides (2000)

A general strategy has been developed for enantioselective synthesis of diverse carbanucleosides. The key step is a Pd(0)-catalyzed enantioselective allylic amination of cis-3,5-dibenzoyloxycyclopent-2-ene 10a with the nucleobase. With guanine-derived nucleobase 13 and chiral ligand 9, a 93−96% ee was obtained, while 6-chloropurine and chiral ligand 8 gave 94% ee. The reaction was followed by a second Pd(0)-catalyzed allylic alkylation with phenylsulfonyl(nitro)methane 6. The nitrosulfone, thus obtained, served as a versatile intermediate for divergent synthesis in which the phenylsulfonyl(nitro)methyl group is a surrogate for the hydroxymethyl side chain. With the guanine-derived nucleobase 13, (−)-carbovir was obtained in only four steps from 10a. With 6-chloropurine as an adenine equivalent, the obtained nitrosulfone intermediate 26 could be converted into both (−)-aristeromycin and (−)-neplanocin A as well as their 2‘,3‘-diepi isomers.
Use of a dynamic <i>in vitro</i> attachment and invasion system (DIVAS) to determine influence of growth rate on invasion of respiratory epithelial cells by group B <i>Streptococcus</i> (2001)

Expression of capsular polysaccharide (CPS) and some surface proteins by group B Streptococcus (GBS) is regulated by growth rate. We hypothesized that precise control of GBS growth, and thus surface-expressed components, could modulate the ability of GBS to invade eukaryotic cells. To test this hypothesis, a dynamic in vitro attachment and invasion system (DIVAS) was developed that combines the advantages of bacterial growth in continuous culture with tissue culture. Tissue culture flasks were modified with inlet and outlet ports to permit perfusion of GBS. Encapsulated type III GBS strains M781 and COH1 and strains COH1-11 and COH1-13 (transposon mutants of COH1 that express an asialo CPS or are acapsular, respectively) were grown in continuous culture in a chemically defined medium at fast mass doubling time (t(d) = 1.8 h) and slow (t(d) = 11 h) growth rates, conditions previously shown to induce and repress, respectively, type III CPS expression. Encapsulated GBS strains invaded A549 respiratory epithelial cells 20- to 700-fold better at the fast than at the slow growth rate, suggesting a role for CPS. However, unencapsulated GBS were also invasive but only when cultured at the fast growth rate, which indicates that GBS invasion is independent of CPS expression and can be regulated by growth rate. Growth rate-dependent invasion occurred when GBS was grown in continuous culture under glucose-defined, thiamine-defined, and undefined nutrient limitations. These results suggest a growth rate-dependent regulation of GBS pathogenesis and demonstrate the usefulness of DIVAS as a tool in studies of host-microbe interactions.
Materials for Optical Instrumentation (1966)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text William L. Wolfe, "Materials for Optical Instrumentation," Appl. Opt. 5, 1871-1872 (1966) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
A CONVENIENT SYNTHESIS OF OXETENE VIA [2<b>+</b>2]CYCLOADDITION REACTION UNDER MICROWAVE IRRADIATION (2002)

ABSTRACT A facile and fast preparation of oxetene has been reported by a microwave mediated [2+2]cycloaddition reaction of steroidal formyl enamides and alkynes in a solventless condition.
Use of Fourier synthesis holography to image through inhomogeneities (1993)

A method for image formation through inhomogeneities is demonstrated. A broad spectral source is decomposed into its Fourier components, and a hologram is recorded at each wavelength through a diffusing medium. When the holograms are synthesized in a computer, a clear image can be formed of the obscured object.
Enhanced cell‐seeding into 3D porous scaffolds by use of magnetite nanoparticles (2005)

To engineer functional tissues, a large number of cells must be successfully seeded into scaffolds. We previously proposed a methodology for tissue engineering using magnetite nanoparticles and magnetic force, which we termed "Mag-TE." In the present study, we applied the Mag-TE technique to a cell seeding process and have termed the technique "Mag-seeding." The cell-seeding efficiency of NIH/3T3 fibroblasts (FBs) by Mag-seeding was investigated using six types of commercially available scaffolds (5 collagen sponges and 1 D,D-L,L polylactic acid sponge) having various pore sizes. FBs were magnetically labeled with our original magnetite cationic liposomes (MCLs), which have a positive surface charge, to improve adsorption onto the cell surface. FBs labeled with MCLs were seeded onto a scaffold, and a magnet (4 kG) was placed under the scaffold. Mag-seeding facilitated successful cell seeding into the deep internal space of the scaffolds. Cell-seeding efficiency increased significantly in all scaffolds when compared to those without magnetic force. Moreover, when a high-intensity magnet (10 kG) was used, cell-seeding efficiency was significantly enhanced. These results suggest that Mag-seeding is a promising approach for tissue engineering.
ppGpp and DksA likely regulate the activity of the extracytoplasmic stress factor σ<sup>E</sup> in <i>Escherichia coli</i> by both direct and indirect mechanisms (2007)

One of the major signalling pathways responsible for intercompartmental communication between the cell envelope and cytoplasm in Escherichia coli is mediated by the alternative sigma factor, sigmaE. sigmaE has been studied primarily for its role in response to the misfolding of outer membrane porins. This response is essentially reactionary; cells are stressed, porin folding is disrupted, and the response is activated. sigmaE can also be activated following starvation for a variety of nutrients by the alarmone ppGpp. This response is proactive, as sigmaE is activated in the absence of any obvious damage to the cell envelope sensed by the stress signalling pathway. Here we examine the mechanism of regulation of sigmaE by ppGpp. ppGpp has been proposed to activate at least two alternative sigma factors, sigmaN and sigmaS, indirectly by altering the competition for core RNA polymerase between the alternative sigma factors and the housekeeping sigma factor, sigma70. In vivo experiments with sigmaE are consistent with this model. However, ppGpp and its cofactor DksA can also activate transcription by EsigmaEin vitro, suggesting that the effects of ppGpp on sigmaE activity are both direct and indirect.
Probiotic microbes: do they need to be alive to be beneficial? (2009)

An essential symbiotic relationship exists between intestinal cells and commensal bacteria within the human gastrointestinal tract. Alteration or absence of this interaction may play a role in the development of human disease. Use of probiotic organisms has yielded improvement of certain medical conditions, such as inflammatory and infectious gastrointestinal disease, although the mechanisms of benefit remain poorly defined. The administration of live organisms is not without risk, both potential and realized, particularly in certain populations. Therefore, it is of considerable interest to determine if the health benefits of probiotics can be attained without the risks associated with administration of a live organism. Reviewed here is the evidence that heat-killed, ultraviolet-inactivated, and even components of these agents may be just as effective and considerably safer for the host.
Mechanisms of pulmonary vasoconstriction and bronchoconstriction produced by PAF in the guinea‐pig: role of platelets and cyclo‐oxygenase metabolites (1995)

1. The mechanisms of action of platelet activating factor (PAF) in the bronchial and cardiovascular systems have not yet been fully elucidated. In order to characterize better and to ascertain whether the effects of PAF in both these systems may be ascribed to the same mechanisms, we examined the actions of PAF in the heart-lung preparation of guinea-pig (HLP). The role of platelets and of cyclo-oxygenase metabolites was investigated. 2. In HLPs perfused with autologous blood, bolus injections of PAF (4-32 ng) produced major effects at the pulmonary vascular and bronchial levels. Both dose-related pulmonary vascular hypertension and bronchoconstriction produced by PAF were diminished to the same extent (46% and, respectively, 47%) when HLPs were perfused with a medium consisting of homologous red blood cells suspended in physiological solution containing 3.5% dextran (RBC). This suggests that the effects of PAF partially depend on the presence of formed elements. 3. When indomethacin (30 microM) was added to the perfusing blood, the dose-response curve for the pulmonary hypertensive responses produced by PAF was strongly reduced (90%) in comparison to control preparations, whereas the bronchoconstrictor effects of PAF were only partially diminished (23%). These data constitute direct evidence that products of the cyclo-oxygenase pathway exert a major role in the vascular, rather than in the bronchial actions of PAF. 4. In HLPs perfused with RBC containing indomethacin (30 microM), the pulmonary vascular hypertensive responses produced by PAF were almost completely abolished, thus indicating that cyclo-oxygenase products from tissues are involved in these effects. Conversely, PAF administration continued to cause dose-related bronchoconstrictor responses that were reduced only partially in comparison with HLPs perfused with RBC in the absence of the cyclo-oxygenase inhibitor. This implies that PAF also has direct action on the bronchoconstriction evoked.5. At the cardiac level, administration of PAF in HLPs perfused with blood caused a dose-related increase in right atrial pressure accompanied by a decrease in left atrial pressure and cardiac output,which were completely suppressed or attenuated by the absence of formed elements and the addition of indomethacin. This suggests that the progressive heart impairment is secondary to the severe pulmonary hypertension induced by PAF.6. The results of this study performed in the heart-lung preparation of the guinea-pig, which made it possible to simultaneously record cardiovascular and bronchial parameters, indicate that various components are involved in the responses produced by PAF. It is suggested that different mechanisms depending on the relative contribution of these components may account for the PAF-induced effects at the pulmonary vascular and airway levels.
FGF-21 enhances islet engraftment in mouse syngeneic islet transplantation model (2010)

To clarify the effect of fibroblast growth factor-21 (FGF-21) on islet transplantation, a suboptimal number of islets were transplanted into streptozotocin (STZ)-induced diabetic mice with or without FGF-21 treatment. Three-day treatment with FGF-21 contributed to restoration of normoglycemia by suppressing islet graft loss. The FGF-21-treated mice showed lower glycemic levels despite similar insulin content in the graft than that in untreated mice on day 3, indicating that FGF-21 not only has a cytoprotective effect but also decreases β-cell load by increasing insulin sensitivity. These results suggest that FGF-21 may be useful as a treatment to improve islet engraftment rates in clinical islet transplantation.
Long-Term Neuroprotection Achieved with Latency-Associated Promoter-Driven Herpes Simplex Virus Gene Transfer to the Peripheral Nervous System (2005)

We examined the ability of the herpes simplex virus (HSV) latency-associated promoter (LAP2) to drive biologically relevant prolonged transgene expression in the peripheral nervous system. Rat dorsal root ganglia were transduced in vivo by subcutaneous inoculation of replication-incompetent HSV-based vectors containing nerve growth factor (NGF) or neurotrophin-3 (NT-3) under the control of LAP2 (vectors SLN and QLNT3, respectively) and vector SHN expressing NGF under the control of the human cytomegalovirus immediate early promoter. Twenty-four weeks later a pure sensory neuropathy was induced by overdose of pyridoxine (PDX), and the animals were assessed 6 months after inoculation. Inoculation of SLN, but not SHN, attenuated the nerve damage caused by PDX and protected foot sensory amplitude, H-wave amplitude, and behavioral measures of proprioceptive function. QLNT3 was more effective than SLN in preserving the largest myelinated fibers from degeneration. These results indicate that expression of NGF or NT-3 driven by LAP2 is sufficient to prevent the development of neuropathy 6 months after vector inoculation in rats.
Quantitative Analysis of (-)-Epigallocatechin Gallate in Tea Leaves by High-Performance Liquid Chromatography (1991)

The quantitative analysis of (-)-epigallocatechin gallate (EGCG) in tea (Camellia sinensis L.) was performed by high-performance liquid chromatography (HPLC) with a C-18 reversed-phase column. EGCG was then eluted within 20 min by using methanol-water-acetic acid (20 : 75 : 5 (v/v/v)) as an eluent. As an internal standard, tryptophan was used. The content of EGCG in five kinds of green tea (sencha, gyokuro, bancha, matsucha and oolong tea) and in a cup of those was determined by both the extraction method with 50% (v/v) methanol and the infusion method with water. The largest amount of EGCG was obtained from matsucha by the extraction method, or from sencha by the infusion method. Furthermore, EGCG contents in various parts of the tea plant were examined. The first leaf had the highest concentration of EGCG, and the concentration of EGCG decreased with the aging of the leaf.
Sliding Window-Based Erasure Correction Using Biased Sampling (2009)

We study a packet erasure correction method based on biased sampling in a sliding window. This kind of coding works especially well for streaming data, where the data have real-time requirements and expire after sometime. The coding method resembles fountain coding but we allow biased sampling of the source blocks inside the window. We present an exact Markov model for analyzing the erasure correction performance and use Wallenius' noncentral hypergeometric distribution for calculating the erasure correction probability of a specified sampling pattern. The model is derived for independent packet erasures, and the sender is assumed to have an estimate on the erasure probability. We truncate the state space in order to lower the computational complexity by limiting the number of allowable erasures within the window, and study scenarios with low channel erasure probability and small window size. The optimal sampling weights are searched and the results suggest that a deterministic sampling pattern isoptimal in the depicted scenario. A comparison to state-of-the-art Raptor codes for a similar setting reveals improved erasure correction performance with the proposed method.
Compress-Store on Blockchain: A Decentralized Data Processing and Immutable Storage for Multimedia Streaming (2019)

Decentralization for data storage is a challenging problem for blockchain-based solutions as the blocksize plays the key role for scalability. In addition, specific requirements of multimedia data calls for various changes in the blockchain technology internals. Considering one of the most popular applications of secure multimedia streaming, i.e., video surveillance, it is not clear how to judiciously encode incentivization, immutability and compression into a viable ecosystem. In this study, we provide a genuine scheme that achieves this encoding for a video surveillance application. The proposed scheme provides a novel integration of data compression, immutable off-chain data storage using a new consensus protocol namely, Proof-of-WorkStore (PoWS) in order to enable fully useful work to be performed by the miner nodes of the network. The proposed idea is the first step towards achieving greener application of blockchain-based environment to the video storage business that utilizes system resources efficiently.
In vitro solar conversion after the primary light reaction in photosynthesis. Reversible photogalvanic effects of chlorophyll-quinhydrone half-cell reactions (1976)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn vitro solar conversion after the primary light reaction in photosynthesis. Reversible photogalvanic effects of chlorophyll-quinhydrone half-cell reactionsFrancis K. Fong and Nicholas WinogradCite this: J. Am. Chem. Soc. 1976, 98, 8, 2287–2289Publication Date (Print):April 1, 1976Publication History Published online1 May 2002Published inissue 1 April 1976https://pubs.acs.org/doi/10.1021/ja00424a048https://doi.org/10.1021/ja00424a048research-articleACS PublicationsRequest reuse permissionsArticle Views208Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
PRODUCTION IN MONKEYS OF COMPLEMENT-FIXING ANTIBODIES WITHOUT ACTIVE IMMUNITY BY INJECTION OF KILLED PLASMODIUM KNOWLESI (1939)

Injection into rhesus monkeys of Plasmodium knowlesi killed by heat, formalin, drying, or freezing and thawing stimulates the production of complement-fixing antibodies, but no demonstrable agglutinating or protective antibodies are formed. Possible differences in the immunity mechanisms concerned in active infection and in artificial immunization are discussed.
Accurate dispensing system for single oocytes using air ejection (2013)

In this study, we propose a new approach to increase the success rate of single-oocyte dispensing and investigate the subsequent viability of the dispensed oocytes. We used a pair of capacitance sensors placed in a microfluidic chip to detect the oocyte, and custom-designed a special buffer zone in the microchannel to decelerate the flow velocity and reduce the hydraulic pressure acting on the oocyte. In the buffer zone, a semicircular bay, formed by equally spaced micro-pillars, is used to stop the oocyte at the dispensing nozzle hole. Finally, the oocyte is ejected by airflow to the culture array. The novel feature of the developed microfluidic system is that the extraordinary improvement in success rate is accompanied by a lack of change in oocyte survival rate (as assessed by a comparison of survival rates before and after the dispensing procedure). By using this device, we achieved a highly accurate single-oocyte dispensing process with a success rate of 100%. The oocyte survival rate is approximately 70%, regardless of whether or not the oocyte is dispensed. The newly proposed system has the advantages of high operation speed and potential usage for two-dimensional micropatterning.