gebhart/patrepeval huggingface dataset:
Genetic analysis of fusion recombinants in Bacillus subtilis: function of the recE gene. (1990)

Bacillus subtilis protoplast fusion allows the study of the genetic recombination of an entire procaryotic genome. Protoplasts from bacterial strains marked genetically by chromosomal mutations were fused using polyethylene glycol and the regenerated cells analyzed. Recombinants represent 19.3% of heterozygotic cells; they are haploids. Individual characterization of clones show a unique particular phenotype in each colony suggesting that recombination takes place immediately after fusion, probably before the first cellular division. Recombination occurs in the whole chromosome; in one-third of the cases both reciprocal recombinants could be shown in the colony. The genetic interval that includes the chromosome replication origin shows the highest recombination level. Our results suggest that the RecE protein accounts for most of the fused protoplast recombination; however, some "replication origin-specific" recombination events were independent of the recE gene product.
Quantitative Measurement of mRNA Levels by RT-PCR: Studies of ECE-1 Isoforms (2003)

The original description of endothelin-1 (ET-1) included the concept that a novel processing enzyme, referred to as endothelin-converting enzyme (ECE), was required for cleavage of the Trp21–Val22 bond in the biosynthetic intermediate big ET-1 (1). Initially it was thought that ECE could be a chymotrypsinlike enzyme because of the nature of the peptide bond being cleaved (1). The challenge of identifying ECE was soon taken up by many groups and a variety of different proteolytic activities were proposed as potential ECEs (2). By 1990, studies of endogenous ET-1 synthesis by cultured endothelial cells and investigations of the systemic pressor effect of intravenously administered big ET-1 indicated that the physiological ECE was a phosphoramidon-sensitive enzyme (3–5). Phosphoramidon was also shown to inhibit hydrolysis of exogenous big ET-1 by cultured vascular smooth muscle and endothelial cells (6). The pursuit of an ECE that could selectively hydrolyze big ET-1 and was inhibited by phosphoramidon led quickly to the purification and cloning of a metallopeptidase called endothelin-converting enzyme-1 (ECE-1) (7–9). Shortly after this, a second phosphoramidon-sensitive peptidase, with ∼59% structural homology to ECE-1, was cloned and called ECE-2 (10). ECE-1 and ECE-2 are members of a family of type II integral membrane peptidases that also includes neutral endopeptidase 24.11 and the KELL and PEX proteins (11).
API Tank Vapors Project (1993)

Abstract The E&P industry has tens of thousands of field storage tanks in produced oil service. Most of the tanks do not have vapor recovery and the evolved gases are vented to the atmosphere. With the implementation of the 1990 Clean Air Act Amendments (CAAA), the industry is faced with the need to quantify its emissions. These emissions inventories must be broken down by components due to the distinction placed upon the category of Hazardous Air Pollutant (HAP). In addition to HAPs, Volatile Organic Compound (VOC) emissions also need to be quantified for purposes of regulatory reporting and as design basis for vapor recovery units (VRU). Many operators have attempted to measure emissions directly. However high cost and erratic results that can be directly attributed to the nature of the tank system have discouraged the use of field measurement as a viable option for estimating emissions. In June of 1993, an American Petroleum Institute (API) project was initiated to develop an estimation method for HAP and VOC emissions from oil field storage tanks. The method is based on the use of computer process simulation techniques, predicated on minimal and easy-to-acquire field data and laboratory analyses. This paper describes the methodology that will be used and the deliverables that are expected from the joint industry effort.
The Biochemistry of Plants: A Comprehensive Treatise, Volume 6, Proteins and Nucleic Acids (1982)

Book Review| June 01 1982 The Biochemistry of Plants: A Comprehensive Treatise, Volume 6, Proteins and Nucleic Acids The Biochemistry of Plants: A Comprehensive Treatise, Volume 6, Proteins and Nucleic Acids. P. K. STUMPF, E. E. CONN, A. MARCUS. Academic PressNew York and London 1981, pp. 658, £44.35 J. A. BRYANT J. A. BRYANT Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1982 Biochemical Society1982 Biochem Soc Trans (1982) 10 (3): 193–194. https://doi.org/10.1042/bst0100193b Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation J. A. BRYANT; The Biochemistry of Plants: A Comprehensive Treatise, Volume 6, Proteins and Nucleic Acids. Biochem Soc Trans 1 June 1982; 10 (3): 193–194. doi: https://doi.org/10.1042/bst0100193b Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1982 Biochemical Society1982 Article PDF first page preview Close Modal You do not currently have access to this content.
Mucilages and Pectins of Opuntia, Tamarindus and Cydonia (1980)

~~~~~~~~~~~~~~~~ ~~~~~~~~-medica Abstract The polysaccharides of stems of Opuntia Jicusindica, seeds of T amarin­ dus indica and seeds of Cydonia oblonga were found to consist of a mixture of mucilage and pectin. The preparation of each polysaccharide could be achieved in an almost pure state. The mucilage could be extracted with cold aci<lulated water while the pectin was obtained by heating the mare successively at 90° C with water (2 hours), ammonium oxalate solution (1 hour) and acidulated water (2 hours). In each case the polysaccharide was precipa­ tated with ethano l. Each plant organ was specially treated according to its content of foreign sub­ stances prior to extraction of mucilage and pectin. The chemical composition of mucilage and pectin hydrolysates was analysed qualitatively by TLC and quan­ titatively by G LC Differentiation betwe­ en mucilage and pectin of the same plant organ was effected by studying their rela­ tive viscosity, reaction with potassium hydroxide, gel formation and determina- tion of methoxyl group and galacturonic acid contents.
Tuning Forks in Silicon (1989)

The authors describe microfabricated tuning forks in silicon, which are excited electrodynamically. The pressure dependence of the resonance frequency and the Q-factor was measured by an interferometer over a range of pressure 1 mu bar to 2 bar. It is shown that micromachined structures in silicon have Q-factors which are comparable to those of similar quartz structures. >
An Evaluation of the Efficacy and Safety of Tetrahydroaminoacridine (THA) without Lecithin in the Treatment of Alzheimer's Disease (1993)

Seventy-nine patients with probable Alzheimer's disease were enrolled into a double-blind, placebo-controlled cross-over study to assess the therapeutic effect and safety of THA (tetrahydroaminoacridine; tacrine) without concomitant lecithin administration. Forty-one patients completed the trial which consisted of two 12-week treatment phases separated by a 4-week wash-out period. Twenty-six subjects were withdrawn during the active treatment phase, mostly because of elevated transaminases or cholinergic side-effects, and ten during treatment with placebo. Statistical analyses were conducted on two groups of patients; those completing the cross-over and those with at least one evaluation in the first treatment period. This latter analysis, using the last observation carried forward was used to approximate an intention-to-treat analysis. THA was favoured over placebo in all three primary outcome measures (MMSE, ADAS Non-cognitive Scale, and the Functional Life Scale), but the results did not reach statistical significance. THA was favoured over placebo in five of the seven secondary outcome measures, but for only two of these was statistical significance attained. In terms of a three-point or greater increase in MMSE score, three to four times as many subjects improved on THA as on placebo.
Two fibers or one? (a comparison of two-fiber and one-fiber star architectures for fiber-to-the-home applications) (1989)

Issues for consideration when deciding between the two-fiber (2-F) and one-fiber (1-F) architectures for fiber deployment in the distribution portion of the loop are presented. A link-loss assessment of both architectures is presented. 1-F star transmission concerns, namely, directivity and wavelength isolation, are discussed. System upgrade of both architectures is considered. Testing and maintenance are addressed. A cost analysis is presented. The 2-F star is conceptually a simple architecture offering maximum upgrade potential and few transmission and testing concerns. However, its distribution link and life cycle costs exceed that of the 1-F star. The 1-F star offer potential cost savings in fiber cable, splicing, and long-term maintenance. The 1-F star has transmission concerns, however, that could impose limitations on the type of transceivers and other loop components being deployed, and this could result in higher system costs.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Phase III Controlled Evaluation of Glutamine for Decreasing Stomatitis in Patients Receiving Fluorouracil (5-FU)-Based Chemotherapy (1999)

Mucositis is a prominent dose-limiting toxicity associated with 5-FU-based chemotherapy. On the basis of preliminary data suggesting that the amino acid glutamine could alleviate this problem, the authors developed this trial. Patients scheduled to receive their first 5-FU-based chemotherapy regimen were selected for study. Following stratification, patients were randomized, in a double-blind manner, to receive oral glutamine or a placebo preparation in a prophylactic manner. Patients in both groups were given oral cryotherapy before chemotherapy and were evaluated for mucositis by standard physicians' evaluation and by a self-report instrument. Sixty-six patients were randomized to receive glutamine and 68 to receive the placebo preparation. There were no significant differences or substantial trends in the mucositis scores between the two study arms as measured by either the physicians or the patients. It was concluded that the dose and schedule of glutamine used in this clinical trial does not alleviate 5-FU-induced mucositis.
On-chip interconnect modeling by wire duplication (2002)

In this paper, we present a novel wire duplication-based interconnect modeling technique. The proposed modeling technique exploits the sparsity of the L−1 matrix, where L is the inductance matrix, and constructs a sparse and stable equivalent RLC circuit by windowing the original inductance matrix. The model avoids matrix inversions. Most important, it is more accurate and more efficient than many existing techniques.
Leukocyte Extravasation: An Immunoregulatory Role for α- <scp>l-</scp> Fucosidase? (2008)

Fucosylated oligosaccharides and glycoconjugates have been implicated in several biological events, including the cell-cell adhesion processes that mediate inflammation. Alpha-L-fucosidase (ALF) is an exoglycosidase that is involved in the hydrolytic degradation of alpha-L-fucose from glycoconjugates. In this study, we investigated the potential role of ALF in regulation of leukocyte migration. Measurement of transendothelial migration in response to CCL5 demonstrated that pretreatment of monocytic cells with ALF reduced migration (p = 0.0004) to a greater extent than treatment of the endothelial monolayer (p = 0.0374). Treatment with ALF significantly reduced the adhesion of monocytic cells to immobilized P-selectin.Fc. A murine model of experimental autoimmune uveitis was then used to show that treatment of splenic cells with ALF produced an 8.6-fold decrease in rolling and a 3.2-fold decrease in cell migration across the retinal vasculature. Further in vitro studies demonstrated that treatment of monocytes with the chemokines CCL3 or CCL5 increased the level of mRNA encoding ALF; this was accompanied by the detection of significant increases in both the 51- and 56-kDa components of ALF by Western blotting. Treatment of monocytic cells with ALF for 2 h significantly reduced the cell surface expression of CD31, with a further decrease in expression observed after 5 h (p = 0.002). Thus, CD31 and fucosylated ligands of P-selectin seem to be the candidates through which ALF mediates its effect in vitro. These data identify a previously unrecognized immunoregulatory role for ALF in late stages of inflammation.
[Examination of the antioxidative and antidiabetic effect of pomiferin in alloxan-induced diabetes mellitus in an experiment (a pilot study)]. (2007)

The antioxidative and antidiabetic effect of pomiferin was monitored under the experimental conditions of alloxan-induced diabetes mellitus. The animals were divided by random selection into 2 groups (n=7). The treated group was administered pomiferin in peroral doses of 10 mg/kg in Avicel, the placebo group was given only a solution of Avicel, and the last group was intact. Selected laboratory parameters (glucose, urea, cholesterol, antioxidative enzymes, total antioxidative capacity, malondialdehyde in serum: diuresis, total glucose and protein losses through urine) were determined. Kidney tissue and pancreas samples were taken for histopathological analysis. The findings included a statistically significant decrease (p < or = 0.01) in blood glucose level, a significant increase (p < or = 0.01) in glutathione peroxidase, total antioxidative capacity (p < or = 0.05) and a significant decrease (p < or = 0.01) in malondialdehyde level in the treated group compared to the placebo group. A highly significant decrease (p < or = 0.01) in diuresis, glucose and protein losses through urine were identified in the treated group compared to the placebo group. The superoxide dismutase catalytic activity, urea and cholesterol levels involved nonsignificant changes. The results of biochemical examination show a protective antioxidative and antidiabetic effect of pomiferin. The results of histopathological examination correlate with them only partially.
Optimal Design and Performance Analysis of Permanent Magnet Assisted Synchronous Reluctance Portable Generators (2013)

In this paper, design and performance analysis of robust and inexpensive permanent magnet-assisted synchronous reluctance generators (PMa-SynRG) for tactical and commercial generator sets is studied. More specifically, the optimal design approach is investigated for minimizing volume and maximizing performance for the portable generator. In order to find optimized PMa-SynRG, stator winding configurations and rotor structures are analyzed using the lumped parameter model (LPM). After comparisons of stator windings and rotor structure by LPM, the selected stator winding and rotor structure are optimized using a differential evolution strategy (DES). Finally, output performances are verified by finite element analysis (FEA) and experimental tests. This design process is developed for the optimized design of PMa-SynRG to achieve minimum magnet and machine volume as well as maximum efficiency simultaneously.
The Theory of Hurricanes (1991)

The field of fluid mechanics is rapidly advancing, driven by unprecedented volumes of data from experiments, field measurements, and large-scale simulations at multiple spatiotemporal scales. Machine learning (ML) offers a wealth of techniques to extract ...Read More
High sensitivity laser induced fluorescence detection in capillary zone electrophoresis (1988)

Abstract Laser induced fluorescence is used for the detection of labeled amino acids. A preliminary comparison is made of three fluorescence pre‐column labeling reagents, ortho ‐phthaldialdehyde, naphthalene dicarboxaldehyde, and fluorescein isothiocyanate, and data on phenylalanine detection limits are given.
Synthesis of a Soluble n-Type Cyano Substituted Polythiophene Derivative:  A Potential Electron Acceptor in Polymeric Solar Cells (2007)

A novel, easy processable n-type polythiophene derivative poly(3-cyano-4-hexylthiophene) (P3CN4HT) was synthesized and characterized with different spectroscopic techniques such as 1H NMR, size exclusion chromatography, Fourier transformed infrared spectroscopy (FT-IR), UV−vis, photoluminescence, and cyclic voltammetry. P3CN4HT is very soluble in common organic solvents (tetrahydrofyran, chloroform) and has high electron affinity. Systematic photoluminescence measurements were used to characterize several electron donating polymers such as poly(2-methoxy-5-[3‘,7‘-dimethyloctyloxy]-p-phenylene vinylene) (MDMO-PPV), regioregular poly(3-octylthiophene) (P3OT), and poly(4,4‘-dihexylcyclopentadithiophene) (PCPDT). When P3CN4HT was employed in blends as the electron acceptor, we observed complete photoluminescence quenching for both MDMO-PPV:P3CN4HT and P3OT:P3CN4HT mixtures. Preliminary photovoltaic measurements demonstrated power conversion efficiency as high as 0.014% for the MDMO-PPV:P3CN4HT blend without any solvent screening, thickness optimation, or post-fabrication annealing of the devices.
Generic support for caching and disconnected operation (2002)

Current systems use caching to provide high performance and possibly support disconnected operation. However, current solutions are type specific. With the advent of modular distributed operating systems and mobile machines there is a need to provide generic support for implementing cacheable/disconnectable services. We present an architecture that can be used to implement cacheable/disconnectable services of any type. In addition we present the implementation of this architecture on the Spring operating system. We have used this architecture for implementing file caching and are currently working on other caching implementations and providing support for disconnected operation.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
2,5‐diamino‐6‐ribitylamino‐4(3<i>H</i>)‐pyrimidinone 5′‐phosphate synthases of fungi and archaea (2008)

The pathway of riboflavin (vitamin B2) biosynthesis is significantly different in archaea, eubacteria, fungi and plants. Specifically, the first committed intermediate, 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, can either undergo hydrolytic cleavage of the position 2 amino group by a deaminase (in plants and most eubacteria) or reduction of the ribose side chain by a reductase (in fungi and archaea). We compare 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases from the yeast Candida glabrata, the archaeaon Methanocaldococcus jannaschii and the eubacterium Aquifex aeolicus. All three enzymes convert 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate into 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate, as shown by 13C-NMR spectroscopy using [2,1',2',3',4',5'-13C6]2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate as substrate. The beta anomer was found to be the authentic substrate, and the alpha anomer could serve as substrate subsequent to spontaneous anomerisation. The M. jannaschii and C. glabrata enzymes were shown to be A-type reductases catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate. These results are in agreement with the known three-dimensional structure of the M. jannaschii enzyme.
Cross-Linking Density Effect of Fluorinated Aromatic Polyethers on Transport Properties (2009)

Novel sulfonated poly(arylene ether) copolymers containing cross-linking moiety and fluorine atoms were prepared for proton exchange membranes of fuel cells. The copolymers were synthesized using potassium 2,5-dihydroxybenzenesulfonate (SHQ), 4,4′-(hexafluoroisopropylidene)diphenol (6F-BPA), decafluorobiphenyl (DFBP), and 1-ethynyl-2,4-difluorobenzeneand (CM) as a cross-linking moiety through polycondensation. The chemical structures of the cross-linkable copolymers were analyzed by 1H NMR, 19F NMR, and FTIR-ATR spectra. Cross-linked membranes are obtained by thermal curing of the cross-linkable copolymers. The thermal properties of the cross-linked membranes were characterized by TGA and DSC. Intrinsic viscosity and gel fraction were also evaluated in order to measure the molecular weight and cross-linking density, respectively. The cross-linked network structure greatly suppressed the water uptake and swelling of the cross-linked membrane. The proton conductivity and methanol permeability of the membranes decreased with increasing cross-linking moiety. The decreases in the proton conductivity and methanol permeability are mainly caused by the enhanced barrier properties of the membranes due to the introduction of CM. In other words, the membranes blocked the channels for the passage of water and methanol. The proposed membranes showed moderate proton conductivity and significantly lower methanol permeability when compared to Nafion 212. The SFPE90-CM20 membrane in particular showed low water uptake (37.5%), high proton conductivity (0.091 S/cm), and low methanol permeability (37 × 10−8 cm2/s). The selectivities of all the cross-linked membranes, that is, the ratio of proton conductivity to methanol permeability, fell in the range of 122 × 103−587 × 103 S·s/cm3, and they were thus also much higher than the selectivity of Nafion 212 (56 × 103 S·s/cm3).
Dynamic simulation and performance of an electro-rheological clutch based reciprocating mechanism (1999)

A reciprocating mechanism which utilizes two electro-rheological clutches is described. An industrial application of the mechanism is in winding filaments onto bobbins. The required traverse speed is 5 m s-1 with a turn-round period of 10-20 ms, the traverse length is 250 mm and the turn-round position must be electronically controllable and repeatable within the ±1 mm. These combined criteria of high-speed and controllability makes the use of electro-rheological fluids an attractive proposition. The operation of the reciprocating mechanism and the dynamic model used to simulate the performance are outlined. The simulation is verified by comparison with experimental results from a prototype mechanism. Simulations are made to illustrate the effect of various fundamental electro-rheological fluid characteristics, such as electro-shear stress, time delays and viscosity. These simulations are considered in relation to the requirements for the operation of the high-speed mechanism.
An OriP/EBNA‐1‐based baculovirus vector with prolonged and enhanced transgene expression (2006)

The improved baculovirus vector could mediate prolonged and enhanced foreign gene expression in some mammalian cells. Furthermore, an adequate level of the EBNA-1 protein was essential for the maintenance of the OriP-containing baculovirus genome. The new vector has potential for use in gene therapy.