gebhart/patrepeval huggingface dataset:
Major and Minor Capsid Proteins of Human Polyomavirus JC Cooperatively Accumulate to Nuclear Domain 10 for Assembly into Virions (2004)

The human polyomavirus JC (JCV) replicates in the nuclei of infected cells. Here we report that JCV virions are efficiently assembled at nuclear domain 10 (ND10), which is also known as promyelocytic leukemia (PML) nuclear bodies. The major capsid protein VP1, the minor capsid proteins VP2 and VP3, and a regulatory protein called agnoprotein were coexpressed from a polycistronic expression vector in COS-7 cells. We found that VP1 accumulated to distinct subnuclear domains in the presence of VP2/VP3 and agnoprotein, while VP1 expressed alone was distributed both in the cytoplasm and in the nucleus. Mutation analysis revealed that discrete intranuclear accumulation of VP1 requires the presence of either VP2 or VP3. However, VP2 or VP3 expressed in the absence of VP1 showed diffuse, not discrete, nuclear localization. The C-terminal sequence of VP2/VP3 contains two basic regions, GPNKKKRRK (cluster 1) and KRRSRSSRS (cluster 2). The deletion of cluster 2 abolished the accumulation of VP1 to distinct subnuclear domains. Deletion of the C-terminal 34 residues of VP2/VP3, including both cluster 1 and cluster 2, caused VP1 to localize both in the cytoplasm and in the nucleus. Using immunoelectron microscopy of cells that coexpressed VP1, VP2/VP3, and agnoprotein, we detected the assembly of virus-like particles in discrete locations along the inner nuclear periphery. Both in oligodendrocytes of the human brain and in transfected cells, discrete nuclear domains for VP1 accumulation were identified as ND10, which contains the PML protein. These results indicate that major and minor capsid proteins cooperatively accumulate in ND10, where they are efficiently assembled into virions.
The chicken B‐cell receptor complex and its role in avian B‐cell development (2000)

The bursa of Fabricius is critical to normal B-lymphocyte development in birds. During embryonic life, B-cell precursors migrate to the bursal rudiment and those which have undergone productive V(D)J recombination colonize lymphoid follicles and undergo immunoglobulin V gene diversification by gene conversion. The chicken surface IgM complex appears structurally and functionally equivalent to its mammalian counterpart, with homologs to CD79a and CD79b. Expression of a truncated Igmu chain is sufficient to drive the early stages of B-cell development in the embryo bursa. Bursal cells expressing the truncated mu receptor complex proliferate in bursal follicles, and those which contain V gene rearrangements undergo V gene diversification by gene conversion. The bursa is a gut-associated organ and antigen is focused to bursal lymphoid follicles after hatch. While expression of the truncated mu chain is sufficient to support B-cell development in the embryo, B cells expressing this receptor are rapidly eliminated after hatch. We suggest the possibility that B-cell development in the bursa after hatch is driven by encounter with antigen leading to redistribution of B cells within the lymphoid follicle, B-cell proliferation and V gene repertoire development by gene conversion.
A Security Framework for Input Validation (2008)

Input manipulation attacks are becoming one of the most common attacks against Web applications and Web services security. As the use of firewalls and other security mechanisms are not effective against application-level attacks, new means of defense are needed. This paper presents a framework proposal to solve this problem, securing applications against input manipulation attacks. The proposed mechanism offers a reusable approach by the use of XML files and a XML Schema for security parameters specification. Furthermore, a case of study and experiment results are presented. The experiment demonstrates how common input manipulation flaws could be observed.
New synthesis of maltol (1974)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew synthesis of maltolRajender K. Chawla and William E. McGonigalCite this: J. Org. Chem. 1974, 39, 22, 3281–3282Publication Date (Print):November 1, 1974Publication History Published online1 May 2002Published inissue 1 November 1974https://pubs.acs.org/doi/10.1021/jo00936a026https://doi.org/10.1021/jo00936a026research-articleACS PublicationsRequest reuse permissionsArticle Views428Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Chirped imaging pulses in four-dimensional electron microscopy: femtosecond pulsed hole burning (2012)

The energy and time correlation, i.e. the chirp, of imaging electron pulses in dispersive propagation is measured by time-slicing (temporal hole burning) using photon-induced near-field electron microscopy. The chirp coefficient and the degree of correlation are obtained in addition to the duration of the electron pulse and its energy spread. Improving temporal and energy resolutions by time-slicing and energy-selection is discussed here and we explore their utility in imaging with time and energy resolutions below those of the generated ultrashort electron pulse. Potential applications for these imaging capabilities are discussed.
Laboratory diagnosis of<i>Mycoplasma pneumoniae</i>infection. 4. Antigen capture and PCR-gene amplification for detection of the mycoplasma: problems of clinical correlation (1992)

Direct detection assays for Mycoplasma pneumoniae were established by PCR amplification of short sequences within the foot protein/adhesin (P1) gene and the 16S ribosomal RNA gene. Specificity and sensitivity was excellent, no hybridization was observed with M. genitalium and other human Mycoplasma species. In nose and throat washings from subjects with respiratory infection a pattern of high counts (c.f.u./ml) of M. pneumoniae (deduced from the amount of amplified PCR product), and a positive antigen capture assay, was found in 83% of subjects with serological evidence of current infection with M. pneumoniae. A small proportion of subjects with serological patterns suggesting infection in the more distant past had positive PCR assays. This was considered to represent either persistence of the organism from a previous infection or perhaps transient carriage during a reinfection, without substantial change in antibody response. PCR-based assay of M. pneumoniae offers a powerful, rapid, and sensitive substitute for culture of the mycoplasma. Antigen capture, while less sensitive than PCR, offers the advantage that it is more often positive with samples from current infection and requires less stringent laboratory organization to contain false positive results. We conclude however that the laboratory diagnosis of a chosen clinical episode should not rest on the PCR or Ag-EIA assays alone, but must also include antibody assays to confirm whether infection is current or represents persistence from past exposure.
Experimental WDM upgrade of a PON using an arrayed waveguide grating (1996)

An all-optical passive WDM high capacity upgrade of a passive optical network (PON) is proposed and experimentally verified, using an arrayed waveguide grating (AWG) and direct detection receivers. The unmodified outside fiber plant and the AWG can be supervised from the central office (CO).
Hydrazine and Thermal Reduction of Graphene Oxide: Reaction Mechanisms, Product Structures, and Reaction Design (2009)

The density functional theory method (M05-2X/6-31G(d)) was used to investigate reaction mechanisms for deoxygenation of graphene oxides (GOs) with hydrazine or heat treatment. Three mechanisms were identified as reducing epoxide groups of GO with hydrazine as a reducing agent. No reaction path was found for the hydrazine-mediated reductions of the hydroxyl, carbonyl, and carboxyl groups of GO. We instead discovered the mechanisms for dehydroxylation, decarbonylation, and decarboxylation using heat treatment. The hydrazine de-epoxidation and thermal dehydroxylation of GO have opposite dependencies on the reaction temperature. In both reduction types, the oxygen functionalities attached to the interior of an aromatic domain in GO are removed more easily, both kinetically and thermodynamically, than those attached at the edges of an aromatic domain. The hydrazine-mediated reductions of epoxide groups at the edges are suspended by forming hydrazino alcohols. We provide atomic-level elucidation for the deoxygenation of GO, characterize the product structures, and suggest how to optimize the reaction conditions further.
A modified quasi-Yagi planar antenna with wideband characteristics in C-band (2002)

The planar microstrip-fed quasi-Yagi antenna has shown excellent radiation characteristics over a wide frequency band. In this paper, we report a modified version of the quasi-Yagi antenna to further improve its performance. The C-band prototype antenna measures 50% bandwidth (S/sub 11/ < -10 dB) and higher gain (6.5 dB) than the original quasi-Yagi design. The antenna radiates a well-defined endfire beam, with a front-to-back ratio > 20 dB and cross polarization level below -18 dB. The enhanced performances have been confirmed by both FDTD simulations and measurements.
Synthesis of Non-nucleoside Analogs of Toyocamycin, Sangivamycin, and Thiosangivamycin:  Influence of Various 7-Substituents on Antiviral Activity (1996)

A number of 7-substituted 4-aminopyrrolo[2,3-d]pyrimidine-5-carbonitrile, -5-carboxamide, and -5-thiocarboxamide derivatives related to the nucleoside antibiotics toyocamycin and sangivamycin were prepared and tested for their activity against human cytomegalovirus (HCMV) and herpes simplex virus type-1 (HSV-1). Treatment of 2-amino-5-bromo-3,4-dicyanopyrrole (1) with triethyl orthoformate followed by alkylation via the sodium salt method with a variety of alkylating agents furnished the corresponding 1-substituted pyrroles 2a-k. Ring annulation was achieved with methanolic ammonia affording the 7-substituted 4-amino-6-bromopyrrolo++-[2,3-d]pyrimidine-5-carbonitrile derivatives 3a-k. Debromination of 3a-k, via catalytic hydrogenation, gave the corresponding 7-substituted 4-aminopyrrolo[2,3-d]pyrimidine-5-carbonitrile analogs 4a-j,l. A selective reduction of 4-amino-6-bromo-7-allylpyrrolo[2,3-d]-pyrimidine-5-carbon ril e (3k) in zinc and acetic acid furnished 4-amino-7-allylpyrrolo-[2,3-d]pyrimidine-5-carbonitrile (4k). Conventional functional group transformations involving the 5-cyano group of 4 furnished the 5-carboxamide derivatives 5a-1 and the 5-thio-amide analogs 6a-l. A similar transformation of the aglycone of toyocamycin (4m) furnished the corresponding aglycone of thiosangivamycin (6m). Several of the new compounds (4-6a-ej-l) were evaluated for their ability to inhibit the growth of L1210 murine leukemic cells. Whereas a number of the carboxamide (5) and thioamide (6) derivatives had modest activity, the corresponding nitrile analogs (4) were all inactive. All compounds were tested for activity against HCMV and HSV-1. The non-nucleoside nitrile analogs 4a-m and carboxamide derivatives 5a-l were, with a few exceptions, essentially inactive against HCMV and HSV-1 and relatively nontoxic. In direct contrast, nearly all of the thioamide derivates 6a-1, including the aglycone of thiosangivamycin (6m), were good inhibitors of HCMV and HSV-1. Most were noncytotoxic in their antiviral concentration range. Cytotoxicity which was observed appeared to be a consequence of DNA synthesis inhibition. Several of these compounds, such as 6b,e, were particularly interesting inhibitors of HCMV with IC(50)'s ranging from 0.1 to 1.3 muM. The antiviral activity of both compounds was well separated from cytotoxicity in KB, HFF, and L1210 cells.
Effect of the Interfacial Layer Composition on the Properties of Emulsion Creams (2007)

We have quantified observed differences in the microstructure and rheology of creaming emulsions stabilized by protein and low molecular weight surfactants. In this study, we made two sets of emulsions from a single parent emulsion, which differed only in their interfacial composition (i.e., either protein or surfactant). The protein studied was whey protein isolate. The zeta potential of the surfactant-stabilized emulsion was controlled by mixing anionic (SDS) and nonionic (Brij 35) surfactants to match the zeta potential of the protein-stabilized emulsion. Despite this, ultrasonic creaming measurements and confocal microscopy showed that the structures within the cream layers were different between the two sets of emulsions. The protein-stabilized emulsions appeared to slow or arrest the packing within the cream, leading to a lower density network of emulsion droplets, whereas the surfactant emulsion droplets rearranged more quickly into a well-packed, concentrated cream layer. Rheological analysis of the creams showed that despite the protein-stabilized emulsions having a lower dispersed phase volume fraction, their elastic modulus was approximately 30 times greater than that of a comparable surfactant-stabilized emulsion. These differences were caused by the ability of the protein to form a highly viscoelastic interfacial network around the droplets which may include intermolecular covalent cross-links. At close range the adhesive nature of the interaction between the layers contributes to the microstructure and rheology of concentrated emulsions. This is the first time that such well-defined emulsion systems have been studied in detail both noninvasively to look at the impact on creaming and also invasively to look at the impact on bulk rheological properties.
Edge Self-Monitoring for Wireless Sensor Networks (2010)

Local monitoring is an effective mechanism for the security of wireless sensor networks (WSNs). Existing schemes assume the existence of sufficient number of active nodes to carry out monitoring operations. Such an assumption, however, is often difficult for a large-scale sensor network. In this work, we focus on designing an efficient scheme integrated with good self-monitoring capability as well as providing an infrastructure for various security protocols using local monitoring. To the best of our knowledge, we are the first to present the formal study on optimizing network topology for edge self-monitoring in WSNs. We show that the problem is NP-complete even under the unit disk graph (UDG) model and give the upper bound on the approximation ratio in various graph models. We provide polynomial-time approximation scheme (PTAS) algorithms for the problem in some specific graphs, for example, the monitoring-set-bounded graph. We further design two distributed polynomial algorithms with provable approximation ratio. Through comprehensive simulations, we evaluate the effectiveness of our design.
Regulation of the production of the RANTES chemokine by endothelial cells. Synergistic induction by IFN-gamma plus TNF-<i>alpha</i> and inhibition by IL-4 and IL-13. (1995)

Production by endothelial cells of the regulated on activation normal T expressed and secreted chemokine (RANTES) has recently been evidenced during delayed-type hypersensitivity (DTH) reactions and may contribute to the local accumulation of macrophages and CD4+ memory T lymphocytes. To document the mechanism inducing RANTES production in this condition, we analyzed the effect of cytokines known to influence the formation of DTH granulomas. Little or no RANTES was produced after stimulation of HUVEC with IFN-gamma, IL-1 beta, or TNF-alpha. However, the combination TNF-alpha+IFN-gamma induced a strong RANTES production. In situ hybridization experiments with a RANTES probe showed that this synergy was also observed at the mRNA level and that the effect of the combination was mainly to increase the amount of RANTES mRNA per cell. The expression of the luciferase gene under the control of the RANTES gene regulatory elements was analyzed; TNF-alpha and the combination TNF-alpha+IFN-gamma activated the regulatory elements. Sequential treatment of HUVEC with TNF-alpha and IFN-gamma showed that IFN-gamma sensitized HUVEC to the stimulating effect of TNF-alpha. The production of RANTES induced by TNF-alpha+IFN-gamma was partly but significantly inhibited by the Th2-type cytokines IL-4 and IL-13. In contrast, IL-10 had no effect. These results indicate that the microenvironment of DTH granulomas, containing high levels of both TNF-alpha and IFN-gamma, may be responsible for RANTES production by perigranulomatous endothelial cells. Inhibition of this production by Th2-type cytokines may be a mechanism by which these cytokines interfere with the formation of DTH granulomas.
13 September 2014 Field Notes (2015)

Field notes including image and/or video records.
Prototyping digital clay as an active material (2009)

This paper describes a shape input device that extends the behavior of clay as a general model of shape interaction and supports arbitrary material deformation, addition and subtraction at interactive rates. These attributes are implemented in a prototype interface consisting of clay composed of wireless position trackers held together by a binding agent. These trackers provide position data in real time while isosurface extraction reconstructs the surface of the model as the user deforms it. Results are shown of this system behaving as an active material by performing geometry capture, and its utility in an augmented reality environment is discussed.
Physical characteristics of the reconstitution intermediates (RI30 and RI30*) from the 30S ribosomal subunit of Escherichia coli (1981)

The isolated reconstitution intermediates (RI30 and RI30*) from the 30S ribosomal subunit of Escherichia coli were found to contain ten proteins. The sedimentation coefficients, diffusion coefficients, density increments, extinction coefficients, and molecular weights were determined for the reconstitution particles and compared with those obtained from the 16S rRNA under identical buffer conditions. The results show that the binding of the proteins on the 16S rRNA at 4 degrees C does not markedly affect the folding of the RNA molecule. However, upon heating the RI30 particle at 40 degrees C to form the RI30* particle, significant folding of the RNA took place, giving a structure considerably more compact than that of the 16S rRNA or the RI30 particle.
Elektronische und strukturelle Eigenschaften von Plasma-Siliziumnitrid zur Oberflächenpassivierung von siebgedruckten, bifazialen Silizium-Solarzellen (2002)

[no abstract]
Overview of PDEs and Their Regulation (2007)

Contraction and relaxation of vascular smooth muscle and cardiac myocytes are key physiological events in the cardiovascular system. These events are regulated by second messengers, cAMP and cGMP, in response to extracellular stimulants. The strength of signal transduction is controlled by intracellular cyclic nucleotide concentrations, which are determined by a balance in production and degradation of cAMP and cGMP. Degradation of cyclic nucleotides is catalyzed by 3',5'-cyclic nucleotide phosphodiesterases (PDEs), and therefore regulation of PDEs hydrolytic activity is important for modulation of cellular functions. Mammalian PDEs are composed of 21 genes and are categorized into 11 families based on sequence homology, enzymatic properties, and sensitivity to inhibitors. PDE families contain many splice variants that mostly are unique in tissue-expression patterns, gene regulation, enzymatic regulation by phosphorylation and regulatory proteins, subcellular localization, and interaction with association proteins. Each unique variant is closely related to the regulation of a specific cellular signaling. Thus, multiple PDEs function as a particular modulator of each cardiovascular function and regulate physiological homeostasis.
How Secure is TextSecure? (2016)

Instant Messaging has gained popularity by users for both private and business communication as low-cost short message replacement on mobile devices. However, before releases about mass surveillance performed by intelligence services such as NSA and GCHQ and Facebook's acquisition of WhatsApp, most mobile messaging apps did not protect confidentiality or integrity of the messages. A messaging app that claims to provide secure instant messaging and has attracted a lot of attention is TextSecure. Besides numerous direct installations, its protocol is part of Android's most popular aftermarket firmware Cyanogen-Mod. TextSecure's successor Signal continues to use the underlying protocol for text messaging. In this paper, we present the first complete description of TextSecure's complex cryptographic protocol, provide a security analysis of its three main components (key exchange, key derivation and authenticated encryption), and discuss the main security claims of TextSecure. Furthermore, we formally prove that - if key registration is assumed to be secure - TextSecure's push messaging can indeed achieve most of the claimed security goals.
White paint on a hot tin roof (1995)

Cities have a tendency to get warmer in summer as buildings and roads cover the countryside in urban sprawl. Scientists in California have carried out a giant computer simulation which demonstrates that areas of Los Angeles could be cooled by as much as 4{sup o}C by replacing black asphalt roads with paler coloured surfaces, painting roof tops white and planting trees to shade houses. Such a reduction in temperature would significantly reduce air-conditioning bills and air pollution. Although there are still many problems to be answered there is growing interest in taking these kinds of measures to reduce the ``urban island heat effect`` in large cities in the USA. It is not clear at present that all cities would respond in the same way and planting the wrong kinds of trees, those which are high emitters of terpenes and isoprene, could exacerbate pollution problems. Nevertheless, large-scale planting schemes are being planned and put into operation. In Sacramento, for example, a programme to plant 500,000 low-emitting trees by the year 2000 is well under way. In addition, a keener interest is being taken in the solar reflectance of roofing and road building materials. Implemented countrywide it is estimated these measures could save billions of dollars in energy costs. (UK)
Extending YANG with Language Abstractions (2011)

YANG - the NETCONF Data Modeling Language - supports modeling of a
tree of data elements that represent the configuration and runtime
status of a particular network element managed via NETCONF. This memo
suggests to enhance YANG with supplementary modeling features and
language abstractions with the aim to improve the model extensibility
and reuse.
An in vitro model of chronic wound biofilms to test wound dressings and assess antimicrobial susceptibilities (2010)

This wound model emphasizes the potential role of the biofilm phenotype in the observed resistance to antibiotic therapies that may occur in chronic wounds in vivo.