gebhart/patrepeval huggingface dataset:
“Click Chemistry” for the Simple Determination of Fatty‐Acid Uptake and Degradation: Revising the Role of Fatty‐Acid Transporters (2015)

Fatty acids (FAs) have numerous functions in all living organisms, ranging from structural roles and energy production to the biosynthesis of secondary metabolites. Because of the high energy content of exogenous FAs, their acquisition is central of metabolism, and several biological systems are known, although their precise roles are not yet entirely clear. We investigated the roles of FadD (CoA ligase) and FadL (FA transporter) in different bacterial strains by using an improved version of click-chemistry-assisted labelling of azido-FAs. The high sensitivity of this method allows a direct and precise assessment of FA metabolism, and is thus far better suited than growth experiments. Our results show that although FA activation is indeed essential for FA degradation, their transport can be independent of transporters like FadL.
Three-dimensional amorphous tungsten-doped nickel phosphide microsphere as an efficient electrocatalyst for hydrogen evolution (2014)

Amorphous tungsten-doped nickel phosphide (a-WNP) microspheres with three-dimensional (3D) ravine-like nanostructures on the surface were successfully fabricated via a fast and facile grain-mediated electroless method. The resulting W-doped NixP exhibits an outstanding electrocatalytic activity for hydrogen evolution reaction with a low onset potential of −50 mV, overpotential of 110 mV at 20 mA cm−2, and small Tafel slope of 39 mV dec−1. The amorphous architecture and the doping of W are considered to be major contributions for the improvement of catalytic performance. The results in this paper might promote a new route to gain both amorphous and doped materials with special electrochemical properties for advanced catalysts and other devices.
Integrated Sample Cleanup−Capillary Electrophoresis Microchip for High-Performance Short Tandem Repeat Genetic Analysis (2008)

An integrated PCR sample cleanup and preconcentration process is developed for forensic short tandem repeat (STR) analysis using a streptavidin-modified photopolymerized capture gel injector for microchip capillary electrophoresis (microCE). PCR samples generated with one biotinylated primer and one fluorescent primer provide the input to the streptavidin-based affinity capture-microCE device. Monoplex PCR samples processed by the device exhibited approximately 10- to 50-fold increased fluorescence intensities, and DNA profiles generated using 9-plex STR samples displayed approximately 14- to 19-fold higher signal intensities compared to those analyzed using traditional cross injection. Complete STR profiles were obtained with as few as 25 copies of DNA template using the capture-microCE device. Four DNA samples with various degrees of degradation were also tested. Samples analyzed using the capture-microCE device resulted in a significant increase of successful allele detection. The ability of our capture-microCE device and method to remove contaminating ions, to concentrate the sample injection plug, and to eliminate electrokinetic injection bias provides a powerful approach for integrating sample cleanup with DNA separation.
Myoglobin: Inherited Structural Variation in Man (1963)

Ultrafiltrates of human myoglobin from 200 individuals were examined by starch-gel electrophoresis. Only two variants, MbAberdeen and MbAnnapolis, of the commonly occurring myoglobin Mb+ were encountered. Each variant is apparently inherited as an autosomal codominant. Heterozygotes for either mutation lack apparent muscular disease. Combined electrophoresis and chromatography of MbAnnapolis indicate a loss of arginine at a position near the C-terminal portion of the molecule.
Emotions Don't Lie: An Audio-Visual Deepfake Detection Method Using Affective Cues (2020)

We present a learning-based method for detecting real and fake deepfake multimedia content. To maximize information for learning, we extract and analyze the similarity between the two audio and visual modalities from within the same video. Additionally, we extract and compare affective cues corresponding to perceived emotion from the two modalities within a video to infer whether the input video is "real" or "fake". We propose a deep learning network, inspired by the Siamese network architecture and the triplet loss. To validate our model, we report the AUC metric on two large-scale deepfake detection datasets, DeepFake-TIMIT Dataset and DFDC. We compare our approach with several SOTA deepfake detection methods and report per-video AUC of 84.4% on the DFDC and 96.6% on the DF-TIMIT datasets, respectively. To the best of our knowledge, ours is the first approach that simultaneously exploits audio and video modalities and also perceived emotions from the two modalities for deepfake detection.
Tailoring Treatment of Depression for Women Across the Reproductive Lifecycle:<i>The Importance of Pregnancy, Vasomotor Symptoms, and Other Estrogen-Related Events in Psychopharmacology</i> (2008)

Compared with men, women are at increased risk of depression, especially at several reproductive-related lifecycle points. This may be partially due to changing levels of estrogen, a hormone that can affect levels of neurotransmitters and neural proteins. As estrogen levels vary throughout the lifespan, risk of depression in women also varies, and not all treatments are appropriate or effective at all times. In adolescence, onset of depression may be associated with onset of puberty, but treating underage girls with antidepressants can risk suicidality. In females of childbearing age, mood disturbances associated with menstrual cycles signal a risk for later full-blown major depressive disorder. In depressed pregnant and postpartum women, risks of treatment versus risks of nontreatment are intricate and require case-by-case evaluation. In perimenopause, vasomotor symptoms may be harbingers of oncoming depression and also may signal the presence of dysregulated hormones and neurotransmitters. Relieving vasomotor symptoms may be a necessary dimension of treating depression. In postmenopause, response to selected antidepressants may depend on whether the patient is also taking hormone-replacement therapy. To attain optimal outcomes, modern psychopharmacologists must tailor treatment of depression to a woman's reproductive stage of life.
Acrylate based anticorrosion films using novel bis‐phosphonic methacrylates (2008)

Abstract Two novel phosphonated methacrylate monomers were successfully synthesized and subsequently incorportated into adhesion/anticorrosive coatings. Specifically, they were propyl N , N ‐tetramethyl‐bis(phosphonate)‐2‐hydroxyl‐bis(methylene) amine methylmethacrylate (MAC 3 NP 2 ) and 2‐[2,2‐bis(diisopropoxyphosphoryl)ethoxy]‐methylmethacrylate, (MAC 3 P 2 ). The phosphonic forms of each monomer were blended with ∼85% w.w acrylates (tripropyleneglycol‐diacrylate and hexanediol‐diacrylate) and 6% w.w of the photo sensitive initiator Darocur®. Along with a monophosphonic monomer synthesized in a previous publication (MAC 3 P), they were polymerized on Q‐panels under ultraviolet light, and then subject to the salt spray test (ca. 0.5 mol/L NaCl at 35 °C) for a duration of up to 50 days. The results indicate that acrylate blends with low concentration of the bisphosphonic compound MAC 3 P 2 have excellent resistance to corrosion, thus excellent adhesive properties. Importantly, these coatings were formed without the use of a hydrophobic polymer matrix or solvents. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 7972–7984, 2008
Glucocorticoid-mediated Repression of Intercellular Adhesion Molecule-1 Expression in Human Monocytic and Bronchial Epithelial Cell Lines (1993)

Increased expression of the intercellular adhesion molecule-1 (ICAM-1) on cells present in the airways has been suggested to play a role in the pathogenesis of asthma by enhancing airway inflammation. We used the monocytic U937 cell line, both undifferentiated and differentiated to a macrophage-like phenotype, and the bronchial epithelial cell line NCI-H292 as cellular model systems for human monocytes/macrophages and bronchial epithelial cells, respectively, and studied the effects of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and dexamethasone on ICAM-1 expression. Both cell lines expressed the ICAM-1 protein constitutively. In addition, TPA- or 1,25-dihydroxyvitamin D3-mediated differentiation of the U937 cell line into a macrophage-like phenotype was associated with increased expression of ICAM-1. In both cell lines, two ICAM-1 mRNA transcripts were found, and expression was stimulated to a similar degree within 1 to 2 h after addition of TPA. In both cell lines, the anti-inflammatory corticosteroid dexamethasone repressed both constitutive and TPA-stimulated ICAM-1 expression, within 3 h of its addition. In the presence of cycloheximide, a marked superinduction of ICAM-1 was observed, while the repressive effect of dexamethasone remained, supporting the hypothesis that dexamethasone acts directly at the transcriptional level.
The PHA Depolymerase Engineering Database: A systematic analysis tool for the diverse family of polyhydroxyalkanoate (PHA) depolymerases (2009)

The DED is a valuable tool which can be applied to identify new PHA depolymerase sequences from complete genomes in silico, to classify PHA depolymerases, to predict their biochemical properties, and to design enzyme variants with improved properties.
Chemical kinetics of water‐rock interactions (1984)

The recent literature on the kinetics of water‐rock interactions is reviewed. The data are then extended to provide a quantitative framework for the description of weathering and alteration. The available experimental data on dissolution of silicates verifies quantitatively the usual mineral stability series in sedimentary petrology. The rate of hydration of carbonic acid is shown to be a possible limiting factor in water‐rock interactions. The framework is developed to enable use of laboratory dissolution experimental results and thermodynamics to arrive at a rate law applicable up to equilibrium and therefore applicable to natural systems. The kinetic justification for the significance of a water‐rock ratio is discussed. With a proper treatment of fluid flow, the equations are applied to the weathering profile leading to the development of bauxites from nepheline syenites.
Perceived Discrimination and Subjective Well-being among Rural-to-Urban Migrants in China (2013)

Using data from a 2009 national household survey (N = 2,866), this study investigates the differential experience of perceived institutional and interpersonal discrimination among rural-to-urban migrants in China, and the consequences of these two types of discrimination on measures of subjective well-being. The results indicate that rural-to-urban migrants perceive institutional discrimination more frequently than interpersonal discrimination. However, perceived interpersonal discrimination has a more detrimental effect than perceived institutional discrimination for rural-to-urban migrants, and this effect takes the form of self-rated physical health and depressive distress. The research calls for a more equitable social environment and equal distribution of resources and opportunities in China.
The Bluetooth technology: merits and limitations (2002)

Bluetooth/sup TM/ wireless technology is a proposed publicly available specification for radiofrequency (RF), short-range, point-to-multipoint voice and data transfer. It also supports point-to-point connections. It operates in the 2.4 GHz ISM (industrial, scientific and medical) band and is based on a low-cost, short-range radio link that facilitates ad-hoc connections for stationary and mobile communication environments. There has been much interest in the Bluetooth technology since it could enable users to connect to a wide range of computing and telecommunications devices without the need to buy, carry, or connect cables. It delivers opportunities for rapid, ad-hoc connections, and in the future, could possibly enable automatic, unconscious connections between devices. In this paper, we discuss the merits and limitations of the Bluetooth technology and address challenging problems that may arise in the future when using Bluetooth wireless communication. We also present new and interesting open research problems in this area.
Fenomena Peranan Masyarakat dalam Penyelesaian Tindak Pidana di Sulawesi Selatan dalam Perspektif Hukum Progresif dan Pasal 49 Ayat (1) KUHP (2011)

Titik balik dari pertentangan antara hukum secara normatif (dalam arti luas) dengan aplikasinya, gerakan massa hanyalah variabel. Gerakan massa tersebut menunjukkan dan menuntut bahwa tidak ada elemen masyarakat yang harus bebas sebagai pengecualian hukum keadilan. Demikian pula, tidak ada elemen masyarakat yang bersifat elementer bilamana hukum ingin diangkat ke tingkat dignity. Sebab semua elemen masyarakat adalah sama kedudukannya pada tingkat hukum yang dignity itu. Inilah salah satu inti hukum progresif. Yang ada dan yang berbeda adalah ada yang mengatur dan ada yang diatur. Artinya hukum itu mengatur yang mengatur demi keteraturan yang diatur dan konsisten pengatur. Apabila yang yang terjadi adalah ketidakaturan yang disengaja berarti terjadi ketidakadilan. Maka secara normatif dalam arti luas, masyarakat memiliki hak eksepsional untuk melindungi diri sendiri. Karena kosensus hukum antara rakyat (masyarakat negara). Negara dan pemerintah tidak boleh terabaikan apalagi tereliminasikan.
Silicon-Based Plasmonics for On-Chip Photonics (2010)

Silicon-based photonic devices dissipate substantially less power and provide a significantly greater information bandwidth than electronic components. Unfortunately, large-scale integration of photonic devices has been limited by their large, wavelength-scale size and the weak optical response of Si. Surface plasmons may overcome these two limitations. Combining the high localization of electronic waves with the propagation properties of optical waves, plasmons can achieve extremely small mode wavelengths and large local electromagnetic field intensities. Si-based plasmonics has the potential to not only reduce the size of photonic components to deeply subwavelength scales, but also to enhance the emission, detection, and manipulation of optical signals in Si. In this paper, we discuss recent advances in Si-based plasmonics, including subwavelength interconnects, modulators, and emission sources. From scales spanning slab waveguides to single nanocrystals, we show that Si-based plasmonics can enable optical functionality competitive in size and speed with contemporary electronic components.
Mechanism of action of hammerhead ribozymes and their applications <i>in vivo</i>: rapid identification of functional genes in the post-genome era by novel hybrid ribozyme libraries (2002)

A hammerhead ribozyme was demonstrated to be a metalloenzyme. By controlling the metal-binding ability of the hammerhead ribozyme in the presence or absence of a specific sequence of interest, we engineered an allosterically controllable ribozyme, designated the maxizyme. Hybrid ribozymes were then constructed by coupling the site-specific cleavage activity of a hammerhead ribozyme with the unwinding activity of an endogenous RNA helicase. This leads to extremely efficient cleavage of target mRNA, not only in vitro, but also in vivo, and eliminates one of the major problems arising in the application of ribozymes for cleavage of mRNA in vivo: that many target sites on the RNA were previously inaccessible to cleavage owing to secondary and/or tertiary structure formation. Since hybrid ribozymes can efficiently attack target sites within mRNA, libraries were made of hybrid ribozymes with randomized binding arms, which were then introduced into cells. This procedure made it possible to readily identify the relevant genes associated with a specific phenotype, such as in apoptosis and cancer metastasis pathways. This application of a randomized library of hybrid ribozymes represents a simple, yet powerful, method for the identification of genes associated with specific phenotypes in the post-genome era. Moreover, vector-based siRNA (short-interfering RNA for RNA interference, RNAi) can also be used for the creation of the libraries and for the subsequent confirmation of the identified genes, relevant in the examined phenotype.
Thymine methyls and DNA–protein interactions (1987)

Evidence is summarized showing that thymine methyls are as important in the recognition of specific sequences by proteins as are the more widely recognized hydrogen bonding sites of bases in the major groove (1). Strongest evidence has come from experiments using functional group mutagenesis (2) in which thymines in a specific recognition sequence (e.g., promoters, operators and restriction sites) are replaced by oligonucleotide synthesis with methyl-free uracil or cytosine and 5-methylcytosine. Such experiments have shown that thymine methyls can provide contact points via van der Waals interactions with amino acid side chains of specific DNA binding proteins. Actual contact between a thymine methyl and carbons of a glutamine side chain has been observed in a cocrystal of the phage 434 repressor and its operator by X-ray analysis. The issue of why thymine occurs in DNA is discussed in light of these findings.
<title>Investigations on polarization schemes for use in wireless environments</title> (2002)

This paper presents a summary of polarization diversity schemes used in wireless communications. Results of various polarization schemes using dipoles, evaluated with respect to parameters such as SINR, mean effective gain, correlation coefficient etc. are presented. Analytical results show a noticeable improvement of the performance of the polarization diversity scheme when combined with the space diversity scheme.
Detecting treatment response in a model of human breast adenocarcinoma using hyperpolarised [1-13C]pyruvate and [1,4-13C2]fumarate (2010)

The recent introduction of a dynamic nuclear polarisation technique has permitted noninvasive imaging of tumour cell metabolism in vivo following intravenous administration of 13C-labelled cell substrates. Changes in hyperpolarised [1-13C]pyruvate and [1,4-13C2]fumarate metabolism were evaluated in both MDA-MB-231 cells and in implanted MDA-MB-231 tumours following doxorubicin treatment. Treatment of MDA-MB-231 cells resulted in the induction of apoptosis, which was accompanied by a decrease in hyperpolarised 13C label flux between [1-13C]pyruvate and lactate, which was correlated with a decrease in the cellular NAD(H) coenzyme pool. There was also an increase in the rate of fumarate conversion to malate, which accompanied the onset of cellular necrosis. In vivo, the decrease in 13C label exchange between pyruvate and lactate and the increased flux between fumarate and malate, following drug treatment, were shown to occur in the absence of any detectable change in tumour size. We show here that the early responses of a human breast adenocarcinoma tumour model to drug treatment can be followed by administration of both hyperpolarised [1-13C]pyruvate and [1,4-13C2]fumarate. These techniques could be used, therefore, in the clinic to detect the early responses of breast tumours to treatment.
Exosomes Communicate Protective Messages during Oxidative Stress; Possible Role of Exosomal Shuttle RNA (2010)

These results argue that the exosomal shuttle of RNA is involved in cell-to-cell communication, by influencing the response of recipient cells to an external stress stimulus.
La Minéralisation au Katanga méridional (1930)

info:eu-repo/semantics/published
Molecular analysis of the <i>Corynebacterium glutamicum gdh</i> gene encoding glutamate dehydrogenase (1992)

The Corynebacterium glutamicum gdh gene encoding NADP-dependent glutamate dehydrogenase (GDH) has been isolated by complementation of the Escherichia coli gdh mutant PA340. The gdh gene was subcloned into the E. coli/C. glutamicum shuttle vector pEK0 and introduced into C. glutamicum. Recombinant strains showed approximately eightfold higher specific GDH activity (15U mg protein-1) relative to the wild type (1.8U mg protein-1). Physiological studies with wild-type and recombinant C. glutamicum strains revealed no indication of significant regulation of gdh expression. The DNA sequence of 2082 bp, including the gdh gene, 5'-, and 3'-flanking regions, was determined. The structural gene consists of 1344 bp and codes for a polypeptide of 448 amino acid residues (Mr 49,152) showing up to 53.6% identity with reported amino acid sequences of glutamate dehydrogenases from other organisms. Northern blot hybridization revealed a 1.65kb mRNA transcript, indicating that the gdh gene of C. glutamicum is monocistronic. Transcription occurred from a G residue located 284 bp upstream of the AUG considered to be the translational initiation codon.
Vaccination of Colorectal Cancer Patients with Modified Vaccinia Ankara Encoding the Tumor Antigen 5T4 (TroVax) Given Alongside Chemotherapy Induces Potent Immune Responses (2007)

Potent 5T4-specific cellular and/or antibody responses were induced in all evaluable patients and were still detectable during the period in which chemotherapy was administered. These results suggest that TroVax can be added to chemotherapy regimens without any evidence of enhanced toxicity or reduced immunologic efficacy and may provide additional clinical benefit.
Some condensation reactions between aromatic aldehydes and 1,1-bisdialkylaminoalkenes and related compounds (1969)

Condensation of 1,1-bisdialkylaminoethenes with aromatic aldehydes gives the corresponding trans-cinnamoyl amides in fair yield. Salicylaldehyde affords coumarin. 1,1-Bisdialkylamino-1,3-dienes and aromatic aldehydes produce 5-arylpenta-cis-2,trans-4-dienoyl dialkylamides. The mechanisms of these reactions have been explored. Some related condensations using 1-alkoxy-1-dialkylaminoethenes and 1-alkylthio-1-dialkylaminoethenes have also been examined.
Free Vibration Analysis of PZT Glide Heads (1999)

This paper studies natural frequencies and mode shapes of a glide head with a piezoelectric transducer (PZT) through calibrated experiments and a finite element analysis. In the experiments, the PZT transducer served as an actuator exciting the glide head from 100 kHz to 1.3 MHz, and a laser Doppler vibrometer (LDV) measured displacement of the glide head at the inner or outer rail. The natural frequencies were measured through PZT impedance and frequency response functions from PZT to LDV. In the finite element analysis, the glide head was meshed by brick elements. The finite element results show that there are two types of vibration modes: slider modes and PZT modes. Only the slider modes are important to glide head applications. Moreover, natural frequencies predicted from the finite element analysis agree well with the experimental results within 5% of error. Finally, the finite element analysis identifies four critical slider dimensions whose tolerance will significantly vary the natural frequencies: PZT bonding length, wing thickness, slider thickness, and air bearing recess depth.
ChemInform Abstract: Nitrogen Vacancy as the Donor: Experimental Evidence in the Ammonia‐Assisted Molecular Beam Epitaxy of GaN. (1998)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.