gebhart/patrepeval huggingface dataset:
Nuclear Targeting Is Required for Hepatoma-derived Growth Factor-stimulated Mitogenesis in Vascular Smooth Muscle Cells (2001)

We recently identified hepatoma-derived growth factor (HDGF) as a nuclear targeted vascular smooth muscle cell (VSM) mitogen that is expressed in developing vascular lesions. In the present study, VSM in culture express endogenous HDGF only in the nucleus and target a green fluorescent protein (GFP)-HDGF fusion to the nucleus. To define the features of the HDGF molecule that are essential for nuclear localization and mitogenic function, deletion and site-directed mutagenesis were performed. Deletion analysis identified the carboxyl-terminal half of HDGF to be responsible for nuclear targeting in VSM. Overexpression of tagged HDGF proteins with point mutations in the putative bipartite nuclear localization sequence in the carboxyl terminus demonstrated that single Lys --> Asn mutations randomized HDGF expression to both the nucleus and cytoplasm similar to the empty vector. Importantly, the Lys --> Asn mutation of all three lysines blocked nuclear entry. Point mutation of a p34(cdc2) kinase consensus motif within the nuclear localization sequence had no effect on nuclear targeting. Moreover, nuclear entry was essential for the HDGF mitogenic effect, as transfection with the triple Lys --> Asn mutant HA-HDGF significantly attenuated bromodeoxyuridine uptake when compared with transfection with wild type HA-HDGF. We conclude that HDGF contains a true bipartite nuclear localization sequence with all three lysines necessary for nuclear targeting. Nuclear targeting of HDGF is required for HDGF stimulation of DNA replication in VSM.
Mixing Media: Microsoft's 'Movie Maker', Montage and War Poetry in the English Classroom (2005)

The montage task was developed for a Year 10 intermediate class to allow students to experiment with the technique of montage as a vehicle for composing their own war poetry. The task was divided into five stages, which also included collecting still images from Internet search engines and used Microsoft's 'Movie Maker' software to complete their montage sequence, using it as a stimulus to compose their own anthology of war poetry.
Recovery Boiler Superheater Corrosion Field Study (2010)

Abstract With the trend towards increasing the energy efficiency of black liquor recovery boilers in North America, there is a need to utilize superheater tubes with increased corrosion resistance that will permit operation at higher temperatures and pressures. In an effort to identify alloys with improved corrosion resistance under more harsh operating conditions, a field exposure was conducted that involved the insertion of an air-cooled probe, containing six candidate alloys, into the superheater section of an operating recovery boiler. A metallographic examination, complete with corrosion scale characterization using electron microprobe spectroscopy, was conducted after a 1,000 hour exposure period. Based on the results, a ranking of alloys based on corrosion performance was obtained.
Investigation of the Differences in Activity between Hydroxycycloalkyl N1 Substituted Pyrazole Derivatives As Inhibitors of B-Raf Kinase by Using Docking, Molecular Dynamics, QM/MM, and Fragment-Based<i>De Novo</i>Design: Study of Binding Mode of Diastereomer Compounds (2011)

N1 substituted pyrazole derivatives show diverse B-Raf kinase inhibitory activities when different hydroxy-substituted cycloalkyl groups are placed at this position. Docking, molecular dynamics (MD) simulations, and hybrid calculation methods (Quantum Mechanics/Molecular Mechanics (QM/MM)) were performed on the complexes, in order to explain these differences. Docking of the inhibitors showed the same orientation that X-ray crystal structure of the analogous (1E)-5-[1-(4-piperidinyl)-3-(4-pyridinyl)-1H-pyrazol-4-yl]-2,3-dihydro-1H-inden-1-one oxime. MD simulations of the most active diastereomer compounds containing cis- and trans-3-hydroxycyclohexyl substituents showed stable interactions with residue Ile463 at the entrance of the B-Raf active site. On the other hand, the less active diastereomer compounds containing cis- and trans-2-hydroxycyclopentyl substituents showed interactions with inner residues Asn580 and Ser465. We found that the differences in activity can be explained by considering the dynamic interactions between the inhibitors and their surrounding residues within the B-Raf binding site. We also explained the activity trend by using a testing scoring function derived from more reliable QM/MM calculations. In addition, we search for new inhibitors from a virtual screening carried out by fragment-based de novo design. We generated a set of approximately 200 virtual compounds, which interact with Ile463 and fulfill druglikeness properties according to Lipinski, Veber, and Ghose rules.
Checking in on Cds1 (Chk2): A checkpoint kinase and tumor suppressor (2002)

Together, DNA repair and checkpoint responses ensure the integrity of the genome. Coordination of cell cycle checkpoints and DNA repair are especially important following genotoxic radiation or chemotherapy, during which unusually high loads of DNA damage are sustained. In mammalian cells, the checkpoint kinase, Cds1 (also known as Chk2) is activated by ATM in response to DNA damage. The role of Cds1 as a checkpoint kinase depends on its ability to phosphorylate cell cycle regulators such p53, Cdc25 and Brca1. A role for Cds1 in repair is suggested by the finding that it interacts with the Holliday junction resolving activity Mus81. This review focuses on the many questions generated by recent progress in understanding the function and regulation of human Cds1.
Velocity estimation from post‐stack seismic data and density log by a linearized inversion (2002)

PreviousNext No AccessSEG Technical Program Expanded Abstracts 2002Velocity estimation from post‐stack seismic data and density log by a linearized inversionAuthors: Chengshu WangMrinal K. SenKirk D. McIntoshChengshu WangThe University of Texas at Austin, Mrinal K. SenThe University of Texas at Austin, and Kirk D. McIntoshThe University of Texas at Austinhttps://doi.org/10.1190/1.1817417 SectionsAboutPDF/ePub ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InRedditEmail Permalink: https://doi.org/10.1190/1.1817417FiguresReferencesRelatedDetails SEG Technical Program Expanded Abstracts 2002ISSN (print):1052-3812 ISSN (online):1949-4645Copyright: 2002 Pages: 2478 publication data© 2002 Copyright © 2002 Society of Exploration GeophysicistsPublisher:Society of Exploration Geophysicists HistoryPublished: 03 Jan 2005 CITATION INFORMATION Chengshu Wang, Mrinal K. Sen, and Kirk D. McIntosh, (2002), "Velocity estimation from post‐stack seismic data and density log by a linearized inversion," SEG Technical Program Expanded Abstracts : 934-937. https://doi.org/10.1190/1.1817417 Plain-Language Summary PDF DownloadLoading ...
Aminoalkenylbenzenesulfonamides with hypotensive and histamine-releasing properties (1970)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAminoalkenylbenzenesulfonamides with hypotensive and histamine-releasing propertiesMarion T. Dorsett, J. Martin Grisar, Kenneth R. Hickey, Rudolph L. Pohl, William J. Hudak, and Richard E. LewisCite this: J. Med. Chem. 1970, 13, 5, 895–900Publication Date (Print):September 1, 1970Publication History Published online1 May 2002Published inissue 1 September 1970https://pubs.acs.org/doi/10.1021/jm00299a023https://doi.org/10.1021/jm00299a023research-articleACS PublicationsRequest reuse permissionsArticle Views56Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
[Heat production by breast neoplasms. IV. Influence of growth rate and probability of lymphatic dissemination during spontaneous development]. (1975)

Growth rate and heat production of breast carcinomas have been investigated during the natural evolution of 21 untreated cancers, all with small tumour size (greater than or equal to 3 cm) and most of them were treated by surgery (excision of growth, mastectomy). Three original facts have thus been discovered : a) the specific heat power 1 (i.e. the heat quantity generated by the tumour per untis of volume and time, computed from from intramammary temperature and thermal conductivity measurements made using of fluvographic needle probes), is typical of each cancer and re7ains remarkably constant during the growth in spite of themorphological and of the morphological and circulatory changes; b) the tumour doubling time tau2v (calculated from measurements of the tumour size effected at various stages of the evolution by assuming an exponential growth), is univocally related to 1 by a hyperbolic law so that the faster the tumour is growing themore heat generates; c) q is significanty higher and tau2v shorter in all cases where the histological examination has revealed signs of lymphatic dissemination (carcinomatous lymphangitis, lymph node metastases,...). Practically q represents a quantitative criteria useful for setting with objectivity the pre-therapeutical prognosis of cancers clinically classified T1 or T2 and of circumscribed no palpable cancers. Similar but less accurate prognostic information may also be obtained by cutaneous thermography (infrared, liquid crystals) considering the intensity, extent and topography of the skin hyperthermia frequently elicited by the cancer. Thid aid to prognosis of the thermal methods is all the more valuable as the tumour doubling time cannot be evaluated under the customary clinical conditions.
Coercivity enhancement in Dy-free Nd–Fe–B sintered magnets by using Pr-Cu alloy (2014)

The grain boundary phase of Dy-free sintered Nd-Fe-B magnets is modified by using Pr68Cu32 eutectic alloy. The coercivity of the modified magnets reaches 21 kOe, which is the highest value in Dy-free Nd-Fe-B sintered magnets. Microstructural investigations show that a smooth and thick grain boundary layer is formed, and the content of the ferromagnetic elements in the grain boundary layer decreases from 65 at. % to 9 at. %. In addition, the mean grain size (4.5 μm) in the doped sintered magnets is smaller than that (6.5 μm) in the original sintered magnets. The modification in grain boundary and grain size reduces the magnetic interactions among grains and hinders nucleation of reversed magnetic domains, resulting in a coercivity enhancement.
How strong is carbon cycle‐climate feedback under global warming? (2004)

The behavior of the coupled carbon cycle and physical climate system in a global warming scenario is studied using an Earth system model including the atmosphere, land, ocean, and the carbon cycle embedded in these components. A fully coupled carbon‐climate simulation and several sensitivity runs were conducted for the period of 1860–2100 with prescribed IPCC‐SRES‐A1B emission scenario. Results indicate a positive feedback to global warming from the interactive carbon cycle, with an additional increase of 90 ppmv in the atmospheric CO 2 , and 0.6 degree additional warming, thus confirming recent results from the Hadley Centre and IPSL. However, the changes in various carbon pools are more modest, largely due to the multiple limiting factors constraining terrestrial productivity and carbon loss. The large differences among the three models are manifestations of some of the poorly constrained processes such as the global strength of the CO 2 fertilization effect and the turnover time and rates of soil decomposition.
The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice (1996)

The human cytomegalovirus (HCMV) major immediate-early promoter (MIEP) is one of the first promoters to activate upon infection. To examine HCMV MIEP tissue-specific expression, transgenic mice were established containing the lacZ gene regulated by the MIEP (nucleotides -670 to +54). In the transgenic mice, lacZ expression was demonstrated in 19 of 29 tissues tested by histochemical and immunochemical analyses. These tissues included brain, eye, spinal cord, esophagus, stomach, pancreas, kidney, bladder, testis, ovary, spleen, salivary gland, thymus, bone marrow, skin, cartilage, and cardiac, striated and smooth muscles. Although expression was observed in multiple organs, promoter activity was restricted to specific cell types. The cell types which demonstrated HCMV MIEP expression included retinal cells of the eye, ductile cells of the salivary gland, exocrine cells of the pancreas, mucosal cells of the stomach and intestine, neuronal cells of the brain, muscle fibers, thecal cells of the corpus luteum, and Leydig and sperm cells of the testis. These observations indicate that the HCMV MIEP is not a pan-specific promoter and that the majority of expressing tissues correlate with tissues naturally infected by the virus in the human host.
New Anticoagulants (2010)

T hromboembolism involving the arterial or venous circu- lation or arising from the heart is a common cause of morbidity and mortality. Rapidly acting parenteral anticoagulants, such as heparin, are used for the prevention and initial treatment of thromboembolism and during revascularization procedures, 1 whereas the slower-acting vitamin K antagonists (VKAs) are used for long-term therapy. Development of new oral anticoagulants to replace VKAs has been slower than that of parenteral agents. Ximelagatran, an oral thrombin inhibitor, was briefly licensed in Europe but was withdrawn in 2006 because of potential hepatic toxicity. lthough this set the field back for several years, the situation has changed with the recent introduction of dabigatran etexilate, a new oral thrombin inhibitor, and rivaroxaban, an oral factor Xa (fXa) inhibitor. Licensed in Europe and Canada for prevention of venous thromboembolism (VTE) in patients undergoing hip or knee arthroplasty, dabigatran etexilate and rivaroxaban streamline out-of-hospital thromboprophylaxis because the drugs can be given once daily in fixed doses without coagulation monitoring. The greater unmet medical need, however, is to find a replacement for VKAs for long-term therapy, particularly stroke prevention in patients with atrial fibrillation (AF). The results of the Randomized Evaluation of Long-Term Anticoagulant Therapy (RE-LY) trial, which compared dabigatran etexilate with warfarin for stroke prevention in patients with AF, demonstrate that the new oral anticoagulants have the potential to be more effective and safer than VKAs.
Secular trends in the growth of children aged 0–6 years in a rural Nigerian community (1996)

Cross-sectional anthropometric measurements were taken between 1987 and 1990 in 673 children aged 0-6 years in Imesi Ile village, Nigeria. The height and weight curves obtained fall behind corresponding growth curves in the same community three decades previously. Mid-upper arm circumference values indicated moderate to severe malnutrition in 25.8% of children aged 1-5 years. The heights of 67.1% and weights of 59.7% of the children fell below the third percentile of a Nigerian equivalent of an international reference population standard. Possible reasons for deterioration of the nutritional status of children in the community include the prevailing adverse socio-economic conditions, changes in child rearing practices over the years, persistence of unsatisfactory living conditions and failure to sustain the implementation of innovative programmes previously introduced into the community. It is suggested that present programmes aimed at promoting the health of rural communities should be reviewed to improve sustainability and should be backed up by measures to improve socio-economic and living conditions.
Inhibitory effect of gymnemic acid on intestinal absorption of oleic acid in rats (1998)

Gymnemic acid, a mixture of triterpene glycosides extracted from the leaves of Gymnema sylvestre, is known to inhibit the intestinal absorption of glucose in human and rats. This work examined the effect of gymnemic acid on oleic acid absorption by the method of intestinal perfusion in rats. The results showed the following. (i) Gymnemic acid potently inhibited the absorption of oleic acid in intestine. (ii) This inhibition was dose dependent and reversible. (iii) The extent of inhibition and the recovery progress were extremely similar to that of glucose absorption. (iv) Taurocholate did not affect the inhibitory effect of gymnemic acid on oleic acid absorption, but lowering its concentration facilitated the recovery from the inhibition. (v) The absorption of oleic acid was not affected by other glycosides such as phloridzin, stevioside, and glycyrrhizin. These new findings are important for understanding the roles of gymnemic acid in therapy of diabetes mellitus and obesity.Key words: gymnemic acid, oleic acid, glucose, intestinal absorption, rat.
Modelling mutational and selection pressures on dinucleotides in eukaryotic phyla –selection against CpG and UpA in cytoplasmically expressed RNA and in RNA viruses (2013)

Methods we have developed identify mutational processes and selection pressures in organisms that provide new insights into nucleotide compositional constraints and a wealth of biochemical and evolutionarily testable predictions for the future.
Development of a new interchangeable virtual instrument class specification (2006)

In today's world, rapidly changing technology and high costs associated with developing and maintaining ATE software push the need for reusability and ease of upgrading or replacing components in test systems intended to be used over a long period of time. The interchangeable virtual instrument (IVI) foundation is tackling these issues by: "Promoting specifications for programming test instruments that simplify interchangeability, provide better performance, and reduce program development and maintenance cost". An interchangeable virtual instrument (IVI) class specification defines the base capabilities and extensions for an instrument type (such as a function generator or oscilloscope). This specification defines the application programming interface (API) used to develop an IVI class-compliant driver. Systems & Electronics Inc. and Boeing are jointly chairing an IVI foundation working group to define an IVI class specification for a counter/timer. Based on the experiences of characterizing the counter/timer specification, this paper explores the process of creating a new class specification for an instrument type that does not already have a specification defined. Topics such as steps to effective specification development, obstacles encountered, and how to keep the specification moving forward are discussed
III–V surface plasma nitridation: A challenge for III–V nitride epigrowth (1999)

GaAs (001), GaP (001), and InP (001) surfaces were exposed to N2 and N2–H2 plasmas in order to investigate the substrate nitridation process. In situ real time ellipsometry was used to assess the nitridation self-limiting kinetics. Ex situ x-ray photoelectron spectroscopy and atomic force microscopy fingerprint the surface composition and morphology, respectively. For GaAs, N2 plasma nitridation forms a GaN layer whose thickness is limited by the As segregation at the GaAs/GaN interface. For GaP and InP substrates, a phosphorous nitride capping layer was formed which inhibits further nitridation. For all substrates, the H2 addition to N2 plasmas results in an increase of the nitridation depth since hydrogen favors the desorption of AsHx and PHx species thereby reducing the inhibition effect of both the As segregation and phosphorus nitride formation.
Plasmin-mediated Release of the Guidance Molecule F-spondin from the Extracellular Matrix (2001)

Serine proteases are implicated in a variety of processes during neurogenesis, including cell migration, axon outgrowth, and synapse elimination. Tissue-type plasminogen activator and urokinase-type activator are expressed in the floor plate during embryonic development. F-spondin, a gene also expressed in the floor plate, encodes a secreted, extracellular matrix-attached protein that promotes outgrowth of commissural axons and inhibits outgrowth of motor axons. F-spondin is processed in vivo to yield an amino half protein that contains regions of homology to reelin and mindin, and a carboxyl half protein that contains either six or four thrombospondin type I repeats (TSRs). We have tested F-spondin to see whether it is subjected to processing by plasmin and to determine whether the processing modulates its biological activity. Plasmin cleaves F-spondin at its carboxyl terminus. By using nested deletion proteins and mutating potential plasmin cleavage sites, we have identified two cleavage sites, the first between the fifth and sixth TSRs, and the second at the fifth TSR. Analysis of the extracellular matrix (ECM) attachment properties of the TSRs revealed that the fifth and sixth TSRs bind to the ECM, but repeats 1-4 do not. Structural functional experiments revealed that two basic motives are required to elicit binding of TSR module to the ECM. We demonstrate further that plasmin releases the ECM-bound F-spondin protein.
Using Moving Average Method to Recognize Systole and Diastole on Seismocardiogram without ECG Signal (2018)

Seismocardiogram (SCG) is a cardio-mechanical signal generated by the heart activities and it can be obtained by placing an accelerometer on the chest. Recently, SCG was used to estimate heart operation by evaluating systolic and diastolic events but the SCG must be coupled to the ECG timing in order to analyze and recognize the events on the SCG waveform. In this study, a low complex algorithm is proposed to identify the regions containing systolic and diastolic points in real time without referencing to the ECG. The method uses the slope, a moving average threshold, and systolic interval constraint to identify the systoles and diastoles. The ECG signal was also collected for manual annotation and comparison. This moving average method has an average error rate of 4% for systolic detection and 9% for diastolic detection on the eight testing subjects. The average processing time of the moving average method is 75.2ms for one-minute data which is suitable for realtime wearable device for healthcare applications.
Zn, Cu and Co in cyanobacteria: selective control of metal availability (2003)

Homeostatic systems for essential and non-essential metals create the cellular environments in which the correct metals are acquired by metalloproteins while the incorrect ones are somehow avoided. Cyanobacteria have metal requirements often absent from other bacteria; copper in thylakoidal plastocyanin, zinc in carboxysomal carbonic anhydrase, cobalt in cobalamin but magnesium in chlorophyll, molybdenum in heterocystous nitrogenase, manganese in thylakoidal water-splitting oxygen-evolving complex. This article reviews: an intracellular trafficking pathway for inward copper supply, the sequestration of surplus zinc by metallothionein (also present in other bacteria) and the detection and export of excess cobalt. We consider the influence of homeostatic proteins on selective metal availability.