gebhart/patrepeval huggingface dataset:
Capillary-Scale Monolithic Immunoaffinity Columns for Immunoextraction with In-Line Laser-Induced Fluorescence Detection (2005)

A bimodal meso/macroporous monolithic silica capillary column containing an entrapped antibody was prepared by a biocompatible sol-gel process and used for nanoflow immunoaffinity chromatography and immunoextraction studies. Stationary phases were prepared by combining the protein-compatible silane precursor diglycerylsilane with an aqueous solution containing 10,000 Da poly(ethylene glycol) and the antibody. An analytical method was developed that was capable of determining both the dissociation constant and binding site content for the anti-fluorescein antibody within the stationary phase. The assay showed that while the antibody residing in macropores was easily removed, approximately 20% of initially loaded antibody remained active and accessible after several washes, consistent with the antibody being entrapped within the mesopores of the sol-gel matrix. The dissociation constants for fluorescein binding to the anti-fluorescein antibody were similar in solution and in the meso/macroporous silica, indicating that the entrapped antibody retained its native conformation within such a matrix. The mixture was loaded into a 250-microm-i.d. fused-silica capillary where the polymer phase separated from the silica followed by gelation of the silica. The capillary-scale immunoaffinity columns could be operated at low back pressure using a syringe pump and were capable of performing chromatographic separations that were dependent on the presence of the antibody within the stationary phase. Such columns could also be operated using in-line laser-induced fluorescence detection. The use of the capillary-scale monolithic columns for on-column immunoextraction and preconcentration is also demonstrated.
35 Translational Control in Poxvirus-infected Cells (2000)

As might be expected, regulation of gene expression occurs at many levels in poxvirus-infected cells. Regulation of the translational machinery primarily involves control of the double-stranded (ds) RNA-activated regulators of translation, PKR and the 2′, 5′ oligoadenylate system; the selective translation of viral mRNA in some cells; and the selective inhibition of host protein synthesis. These aspects of translational control of gene expression in poxvirus-infected cells are covered in this chapter. THE POXVIRIDAE: A BRIEF OVERVIEW Classification and replication of poxviruses has been reviewed recently (Fields et al. 1996). The poxviridae family is made up of large viruses with a (ds)DNA genome 130–300 kbp in length. Poxviral genomes code for more than 100 polypeptides, with the central portion coding for proteins required for virus replication, and the termini coding for proteins involved in virus/host interactions. The family is subdivided into the chordopoxviridae and entomopoxviridae, which infect chordates and arthropods, respectively. The chordopoxviridae are separated into eight recognized genera, based on morphology, antigenic cross-reactivity, and host range. The largest genus is the orthopoxviruses, which contains closely related viruses infecting buffalo, camel, monkey, mouse (ectromelia), rabbit, raccoon, vole, cattle, and humans (smallpox). Vaccinia virus, the type virus of the orthopoxviruses, is the virus that was used to immunize against smallpox in the 20th century. Whereas the original virus used for smallpox “vaccination” by Jenner was derived from a poxvirus that infected cattle (the terms vaccinia and vaccination come from the Latin, vacca , for cow), the virus we now call vaccinia virus...
Proliferation of Rat Pleural Mesothelial Cells in Response to Hepatocyte and Keratinocyte Growth Factors (2000)

The proliferative response of cultured pulmonary mesothelial cells (MCs) to epithelial cell mitogens such as keratinocyte growth factor (KGF) and hepatocyte growth factor (HGF) is investigated. A cell line of rat pleural MCs and freshly prepared rat visceral and parietal MCs were studied. Both KGF and HGF stimulated thymidine uptake in the cell line when cultured for 2 d in serum-free conditions; the growth increase was magnified when tumor necrosis factor (TNF)-alpha was also added to the cultures. Adding asbestos fibers alone to MCs in culture did not enhance DNA synthesis by these cells. The MCs were also shown to synthesize significant amounts of HGF but much less KGF when cultured for 2 d. When freshly prepared MCs were examined, normal cell growth was more rapid in the parietal cells, which also had a more epithelial-type morphology. The addition of HGF and KGF resulted in increased DNA synthesis in each cell type, but no effect of added TNF-alpha was found. The results indicate that pulmonary MCs have the potential to proliferate in response to cytokines such as HGF and KGF that are usually associated with epithelial cell regeneration after injury.
Commentary: Beyond “TRIM” Benefits of β-Glucan by Blood Glucose and Lipid Balancing Potentials in Its Defense Against COVID-19 (2021)

Commentary: Beyond "TRIM" Benefits of β-Glucan by Blood Glucose and Lipid Balancing Potentials in Its Defense Against COVID-19
A System for Detection and Tracking of Human Movements Using RSSI Signals (2018)

A device-free human detection and tracking system using a received signal strength indicator (RSSI) for an indoor environment is presented in this paper. The proposed system has two major functions: a wireless communication system and a human detection and tracking system. The first function is developed for measuring and collecting RSSI signals affected by human presence and movement, while the second function is developed for detecting and tracking the human using a predefined threshold and a zone selection method. The novelty of our proposed system is that the communication protocol can avoid signal interference and packet loss in the network, and the detection and tracking method can specify an actual zone that the human is present by taking an optimal predefined threshold and a level of RSSI variation in each zone into consideration. The proposed system is verified by experiments, and various human movement patterns with different directions and speeds are tested. The experimental results show that the proposed communication protocol can significantly provide communication reliability, and the proposed method can properly detect and track human movements. The packet delivery ratio indicating communication reliability is almost 100%. Detection and tracking accuracy measured by the number of times the method can detect and track the human with the correct zone is almost 100% in all cases of one man movements.
A Fusion Health Monitoring Using ECG and Accelerometer sensors for Elderly Persons at Home (2007)

An ECG and tri-axial accelerometer signal monitoring and analysis method for the homecare of elderly persons or patients, using wireless sensors technology was design and implemented. This paper presents a prototype of wellness monitoring system capable of recording, and analyzing continuous ECG and accelerometer data received from the human body. The system provides an application for recording activities, events and potentially important medical symptoms. The ECG features are used to detect life-threatening arrhythmias, with an emphasis on the software for analyzing the P-wave, QRS complex, and T-wave in ECG signals at server connected to base station which is receiving data from the wireless sensor on the patient body. Activity such as walking and running are detected from the body movements recorded by the accelerometer sensor. IEEE802.15.4 is used for wireless communication between sensor and base station. If any abnormality occurs at server then the alarm condition sends to the doctor' Personal Digital Assistant (PDA).
THE PERCHLORATES OF THE ALKALI AND ALKALINE EARTH METALS AND AMMONIUM. THEIR SOLUBILITY IN WATER AND OTHER SOLVENTS<sup>1</sup> (1923)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE PERCHLORATES OF THE ALKALI AND ALKALINE EARTH METALS AND AMMONIUM. THEIR SOLUBILITY IN WATER AND OTHER SOLVENTS1H. H. Willard and G. Frederick SmithCite this: J. Am. Chem. Soc. 1923, 45, 2, 286–297Publication Date (Print):February 1, 1923Publication History Published online1 May 2002Published inissue 1 February 1923https://pubs.acs.org/doi/10.1021/ja01655a004https://doi.org/10.1021/ja01655a004research-articleACS PublicationsRequest reuse permissionsArticle Views1258Altmetric-Citations63LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
A calculus of atomic actions (2009)

We present a proof calculus and method for the static verification of assertions and procedure specifications in shared-memory concurrent programs. The key idea in our approach is to use atomicity as a proof tool and to simplify the verification of assertions by rewriting programs to consist of larger atomic actions. We propose a novel, iterative proof style in which alternating use of abstraction and reduction is exploited to compute larger atomic code blocks in a sound manner. This makes possible the verification of assertions in the transformed program by simple sequential reasoning within atomic blocks, or significantly simplified application of existing concurrent program verification techniques such as the Owicki-Gries or rely-guarantee methods. Our method facilitates a clean separation of concerns where at each phase of the proof, the user worries only about only either the sequential properties or the concurrency control mechanisms in the program. We implemented our method in a tool called QED. We demonstrate the simplicity and effectiveness of our approach on a number of benchmarks including ones with intricate concurrency protocols.
Fusion and amplification of two originally non‐syntenic chromosomal regions in a mammary carcinoma cell line (1992)

The FLG/FGFRI gene, encoding a receptor for members of the FGF family, is located at 8p11.2-p12. It is amplified, overexpressed, and not grossly rearranged in the MDA-MB-134 breast carcinoma cell line, whereas other genes from the pericentromeric 8p region are not amplified. The FGF4/HSTFI gene, located at 11q13, is also amplified with a substantial portion of the 11q13 region, but is not overexpressed in MDA-MB-134 cells. In this cell line, amplified sequences constitute a large homogeneously staining region (HSR) which is part of a marker chromosome containing chromosome 8 and chromosome 11 sequences. Using probes for the FGF4/HSTFI and the FLG/FGFRI genes in fluorescence chromosomal in situ hybridization, we show that the HSR contains de novo fused and amplified 11q13 and 8p11-p12 sequences associated in a complex structure containing approximately the same number of FGF4 and FGFRI genes. The significance of this genetic abnormality for MDA-MB-134 cells, and for breast carcinogenesis in general, is unknown, but may underlie a particular type of oncogene activation.
Endotaxial Silicide Nanowires (2004)

We demonstrate the growth of self-assembled nanowires of cobalt silicide on Si(111), (100), and (110) substrates during deposition of Co onto a heated Si substrate. Silicide islands form via an endotaxial mechanism, growing into the substrate along inclined Si{111} planes, which breaks the symmetry of the surface and leads to a long, thin nanowire shape. During growth, both the length and width of the islands increase with time in a fixed proportion that varies strongly with growth temperature, which shows that the nanowire shape is kinetically determined. It is expected that nanowires could form in many other overlayer/substrate systems via this mechanism.
Pulse-time-modulated electron cyclotron resonance plasma etching for highly selective, highly anisotropic, and notch-free polycrystalline silicon patterning (1994)

Highly selective, highly anisotropic, and notch-free etching for polycrystalline silicon is achieved in a few tens of μs pulse-time-modulated electron cyclotron resonance plasma. In the pulsed plasma, the selectivity ratio of polycrystalline silicon etching rate to the SiO2 etching rate is drastically improved with high etching rate, as compared with the ratio with a continuous discharge. Furthermore, vertical and notch-free phosphorus-doped polycrystalline silicon etching profiles can be achieved even with 100% over etching. These etching characteristics are achieved by controlling ion energy distributions through the duty ratio, maintaining a high ion current density, generating a collimated ion flux and eliminating surface charging with a pulsed discharge of a few tens of μs.
Network structures and algorithms in Bioconductor (2004)

Packages graph, Rgraphviz, RBGL of Bioconductor (www.bioconductor.org).
DESARROLLAN EN MEDICINA PRUEBA PARA DETECTAR EL MAL DE CHAGAS (2005)

PARA CONTRARRESTAR LOS INDICES DE FALLECIMIENTOS OCASIONADOS POR EL MAL DE CHAGAS, QUE AFECTA A CASI 18 MILLONES DE PERSONAS AMERICA LATINA, CIENTIFICOS DEL LABORATORIO DE BIOLOGIA DE PARASITOS, DEL DEPARTAMENTO DE MICROBIOLOGIA Y PARASITOLOGIA DE LA FACULTAD DE MEDICINA, PRODUJERON UNA SUSTANCIA (ANTIGENO) PARA DIAGNOSTICAR CON TOTAL CERTEZA DICHO PADECIMIENTO. PAZ MARIA SALAZAR SCHETTINO, JEFA DE DICHO LABORATORIO, INFORMO QUE INCLUSO YA PATENTARON ESTA INNOVACION Y YA SE ENCUENTRA PROCESO DE VENTA. LOS LABORATORIOS RESPONSABLES DE ELLO HICIERON UNA TIRA DE DIAGNOSTICO RAPIDO Y AHORA SE ELABORARA LA PRUEBA DE ELISA. SENALO QUE A DIFERENCIA DE OTRAS PRUEBAS DE DIAGNOSTICO EL MERCADO, LA DESARROLLADA LA FACULTAD DE MEDICINA NO CONFUNDE LOS RESULTADOS CON LA ENFERMEDAD DE LEISHMANIA, MUY SIMILAR AL MAL DE CHAGAS. LA ESPECIALISTA EXPLICO QUE LA ACTUALIDAD EXISTEN ENTRE 16 Y 18 MILLONES DE HABITANTES INFECTADOS CON TRYPANOZOMA CRUZI (EL PROTOZOO QUE PRODUCE ESE MAL), Y DE ESTOS, ENTRE 10 Y 40 POR CIENTO TIENEN MANIFESTACIONES CLINICAS. ACLARO QUE EL MAL DE CHAGAS -QUE SOLO SE PRESENTA AMERICA, DESDE MEXICO HASTA ARGENTINA- ES OCASIONADO POR UN MICROORGANISMO PARASITO TRANSMITIDO A LOS HUMANOS A TRAVES DE UN INSECTO CONOCIDO COMO CHINCHE HOCICONA O BESUCONA, MEDIANTE TRANSFUSION SANGUINEA Y, AUNQUE NUMERO REDUCIDO, POR TRASPLANTE DE ORGANOS. REFIRIO QUE ESE ORGANISMO LLEGA A LA SANGRE, GANGLIOS Y OTRAS ZONAS, COMO EL CORAZON, DONDE ANIDA Y PUEDE OCASIONAR COMPLICACIONES, COMO UNA MIOCARDIOPATIA CHAGASICA, DESENCADENANTE DE UNA MUERTE PLENA EDAD PRODUCTIVA, POR LO GENERAL A LOS 40 ANOS, SU MAYOR PERIODO DE INCIDENCIA. DIJO QUE LOS HOMBRES SON MAS PROPENSOS A PADECER LA ENFERMEDAD. EL LABORATORIO SE HICIERON ESTUDIOS CON RATONES, COMPARANDO TESTOSTERONA CONTRA PROGESTERONA Y SE ENCONTRO QUE LOS MACHOS TENIAN MAS LESIONES LOS ORGANOS. ESTE MAL, AGREGO, AFECTA SOBRE TODO A QUIENES VIVEN LA ZONA DEL PACIFICO (OAXACA, GUERRERO Y CHIAPAS). EN EL SUR ES UN PROBLEMA, PERO DESCONOCEMOS LA SITUACION EL NORTE; FALTA MUCHO ESTUDIO, RECONOCIO. SU COMBATE ES CARO, TANTO LO ECONOMICO COMO LO SOCIAL, PORQUE SOLO HAY FARMACOS UTILES PARA LA FASE AGUDA DEL MAL. PARA ENFRENTAR ESTE PADECIMIENTO, PAZ SALAZAR INDICO QUE HAY TRES FACTORES FUNDAMENTALES: EL USO DE INSECTICIDAS, MEJORAR LA VIVIENDA Y EDUCACION PARA LA SALUD.
Comparisons of stuttering frequency during and after speech initiation in unaltered feedback, altered auditory feedback and choral speech conditions (2009)

Altered auditory feedback effectively inhibits stuttering immediately after speech has been initiated. However, unlike a true choral signal, which is exogenously initiated and offers the most complete fluency enhancement, AAF requires speech to be initiated by the user and 'fed back' before it can directly inhibit stuttering. It is suggested that AAF can be a viable clinical option for those who stutter and should often be used in combination with therapeutic techniques, particularly those that aid speech initiation. The substantially higher rate of stuttering occurring on initiation supports a hypothesis that overt stuttering events help 'release' and 'inhibit' central stuttering blocks. This perspective is examined in the context of internal models and mirror neurons.
Bispecific Monoclonal Antibody Therapy of B-Cell Malignancy (1995)

Bispecific monoclonal antibodies (bsAbs) that recognize CD3 with one arm and a tumor associated antigen with the other arm can retarget T-cells toward tumor cells in an MHC independent manner, thereby combining the specificity of monoclonal antibodies with the power of the cellular immune system. B-cell malignancies are particularly attractive as targets for anti-CD3-based bsAb therapy because of their sensitivity to other forms of antibody therapy, and the extent to which B-cells and T-cells communicate at the molecular level. BsAbs that recognize CD3 and a number of antigens on malignant B-cells have been shown in vitro to be capable of retargeting T-cells. In animal models of B-cell malignancy, bsAb can eliminate tumor loads that are resistant to unmodified monoclonal antibody therapy. Ongoing early clinical trials in advanced B-cell lymphoma indicate CD3-based bsAbs have significant biologic effects, and suggest they have anti-tumor activity as well. A number of significant questions relating to bsAb therapy of B-cell malignancies remain. It is unclear what role both endogenously produced and exogenously administered cytokines are likely to play. Further exploration of whether bsAb can induce T-cells to target to tumor will also be required before the true promise of this novel form of immunotherapy can be determined.
Protection of the Lung from Blast Overpressure by Thoracic Stress Wave Decouplers (1996)

(C)British Crown copyright 1996/DERA--published with the permission of the Controller of Her Majesty's Stationery Office. From the Medical Division, Chemical and Biological Defence Establishment, Salisbury, United Kingdom. Presented at the 7th International Symposium of Weapons Traumatology and Wound Ballistics, St. Petersburg, Russia, September 1994. Address for reprints: G. J. Cooper, Trauma Section, Chemical and Biological Defence Establishment, Porton Down, Salisbury, Wiltshire, United Kingdom SP4 0JQ.
Keeping data secret under full compromise using porter devices (2010)

We address the problem of confidentiality in scenarios where the attacker is not only able to observe the communication between principals, but can also fully compromise the communicating parties (their devices, not only their long term secrets) after the confidential data has been exchanged. We formalize this problem and explore solutions that provide confidentiality after the full compromise of devices and user passwords. We propose two new solutions that use explicit key deletion and forward-secret protocols combined with key storage on porter devices. Our solutions provide the users with control over their privacy. We analyze the proposed solutions using an automatic verification tool. We also implement a prototype using a mobile phone as a porter device to illustrate how the solution can be realized on modern platforms.
Methods for reducing the fresnel reflection in an optical‐fiber connector with index matching material (1986)

Abstract If there is a discontinuity of the refractive index in optical fibers connected with an optical connector, the light is reflected from this part to the light source, creating a problem in its light transmission characteristic. The main causes of the discontinuity are: (1) the gap between the endfaces of the optical fibers; and (2) the change of the refractive index at the end‐face of the fibers due to their polishing. Solutions to these problems are investigated in this paper, using silica‐based graded index multimode optical fibers. For (l), a refractive‐index matching material is employed, and the method for selecting the central value of the refractive index and the temperature coefficient from the reflected light in the visual‐light wavelength region is investigated. It has been confirmed that the refractive‐index matching material made of silicone resin and chosen by this method can obtain the required reflection loss greater than 40 dB, in each of the transmission wavelengths of 0.85 pm and 1.3 μm, and in the temperature range of −30°C to +60°C. For (2), the relationship between the polishing conditions and the reflected light power was investigated. It has been found that the refractive index of the endface of an optical fiber changes slightly when polished. The conditions of polishing for suppressing this change have been determined, and the property of the polished endface has been clarified.
Dynamic impact response characteristics of a helmeted Hybrid III headform using a centric and non-centric impact protocol (2012)

A linear impactor system was used to apply a condensed version of the University of Ottawa Test Protocol, employing five centric and non-centric impact conditions, to a Hybrid III headform fitted with six certified ice hockey helmets. None of the helmeted conditions exceeded linear acceleration thresholds for traumatic or mild traumatic brain injury; however, five of the six helmets had angular acceleration results that were above the 80% risk of mild traumatic brain injury threshold proposed by Zhang et al. High risk of mild traumatic brain injury was associated with non-centric impact conditions and peak angular accelerations, supporting the need for improved three-dimensional helmet certification standards.