gebhart/patrepeval huggingface dataset:
Clinical Comparison of the Midvastus and Medial Parapatellar Surgical Approaches (1999)

The medial parapatellar approach and the midvastus approach are the two most commonly used surgical approaches in total knee replacement. This study compared surgical and clinical parameters associated with both surgical approaches in primary total knee replacement. One hundred nine patients who underwent bilateral primary total knee replacements had a medial parapatellar approach to one knee and a midvastus approach to the opposite knee. The prosthetic design and physical therapy were identical in all 109 patients. The patients and physical therapists were blinded to the type of approach used on each knee. The comparison included the surgical parameters of difficulty of exposure, surgical time, incidence of lateral retinacular release, and total blood loss. The clinical parameters of pain, range of motion, ability to perform a straight leg raise, and complications were compared at 8 days, 6 weeks and 6 months. The comparison between the two surgical approaches showed a statistically significant difference in four parameters, all of which favored the midvastus approach. The patients who had the midvastus approach required fewer lateral retinacular releases, had less pain at 8 days, had less pain at 6 weeks, and had a higher incidence of ability to straight leg raise at 8 days. There was no statistical difference between the two surgical approaches in all other surgical and clinical parameters. There was no increased difficulty of exposure using the midvastus approach when compared with the medial parapatellar approach even in patients with severe varus or valgus deformities. Notably, all clinical parameters were equal at 6 months. From a clinical standpoint, the midvastus approach had an advantage over the medial parapatellar approach because the patients had significantly less pain and had the ability to straight leg raise at 8 days. Because the managed care environment dictates a shorter hospital stay, patients who have the midvastus surgical approach have less pain and earlier control of the operative leg, and may be discharged from the hospital earlier. However, the clinical results at 6 months based on the patient's pain relief, range of motion, and ability to straight legraise were identical between the two surgical approaches.
Introduction: The Broader View (2000)

Abstract This introduction to the book discusses the reasons for the expansion of economic thinking into the other social sciences, and argues that an integrated concept of economics and social sciences is not only possible but is indispensable. This is because there is no other way to do justice to the integrated lives of individuals and their choices in trading off objectives in the traditional domains of different disciplines, and no other way to understand all the options for (and implications of) human ambition. Thomas Carlyle's attitude to economics as the ‘dismal science’ is then outlined. The chapter goes on to discuss the content of each of the ten succeeding chapters in detail, under five main headings: broader theories of the firm and the state; the structure of incentives in the modern welfare state; ethnic conflict, discrimination, and coordination in social groups; the state versus the market; and theory, ideology, and cumulative research.
Generation of Human Fab Antibody Libraries: PCR Amplification and Assembly of Light- and Heavy-Chain Coding Sequences (2011)

INTRODUCTION The development of therapeutic antibodies for use in the treatment of human diseases has long been a goal for many researchers in the antibody field. One way to obtain these antibodies is through phage-display libraries constructed from human lymphocytes. This protocol describes the construction of human Fab (fragment antigen binding) antibody libraries. In this method, the individual rearranged heavy- and light-chain variable regions are amplified separately and are linked through a series of overlap polymerase chain reaction (PCR) steps to give the final Fab products that are used for cloning.
Water-borne Polyimides via Microwave-assisted Polymerization (2009)

In the present study, the synthesis of high molecular weight polyimides was conducted in water using microwave irradiation. High molecular weight polymers were obtained after only 5 min of reaction time. The influence of different experimental parameters is discussed in connection with a thorough characterization of the synthesized polymers. From these polymers, self-supporting films were obtained.
A 20 bit logarithmic number system processor (1988)

The architecture and performance of a 20-bit arithmetic processor based on the logarithmic number system (LNS) is described. The processor performed LNS multiplication and division rapidly and with a low hardware complexity. Addition and subtraction in the LNS require the support of a table lookup unit. A scheme is proposed to minimize this complexity using a partitioned memory (ROM) and a PLA (programmable logic array). For performance evaluation, the target technology is integrated Schottky logic. The processor is shown to compare well with, if not to outperform, existing floating point (FLP) processors of equivalent range and precision. The speed-power-product ratio of an equivalent FLP processor, compared with that of the LNS processor, is reported to be 20 to 1 in the case of the square and square-root operation and 1 to 1 in the case of addition and subtraction. For multiplication and division, this ratio is about 5 to 1.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Peptides actively transported across the tympanic membrane: Functional and structural properties (2017)

Otitis media (OM) is the most common infectious disease of children under six, causing more antibiotic prescriptions and surgical procedures than any other pediatric condition. By screening a bacteriophage (phage) library genetically engineered to express random peptides on their surfaces, we discovered unique peptides that actively transport phage particles across the intact tympanic membrane (TM) and into the middle ear (ME). Herein our goals were to characterize the physiochemical peptide features that may underlie trans-TM phage transport; assess morphological and functional effects of phage peptides on the ME and inner ear (IE); and determine whether peptide-bearing phage transmigrate from the ME into the IE. Incubation of five peptide-bearing phage on the TM for over 4hrs resulted in demonstrably superior transport of one peptide, in level and in exponential increase over time. This suggests a preferred peptide motif for TM active transport. Functional and structural comparisons revealed unique features of this peptide: These include a central lysine residue, isoelectric point of 0.0 at physiological pH and a hydrophobic C-terminus. When the optimal peptide was applied to the TM independent of phage, similar transport was observed, indicating that integration into phage is not required. When 109 particles of the four different trans-TM phage were applied directly into the ME, no morphological effects were detected in the ME or IE when compared to saline or wild-type (WT) phage controls. Comparable, reversible hearing loss was observed for saline controls, WT phage and trans-TM peptide phage, suggesting a mild conductive hearing loss due to ME fluid. Perilymph titers after ME incubation established that few copies of trans-TM peptide phage crossed into the IE. The results suggest that, within the parameters tested, trans-TM peptides are safe and could be used as potential agents for noninvasive delivery of drugs, particles and gene therapy vectors to the ME.
Cell- and ligand-specific dephosphorylation of acid hydrolases: evidence that the mannose 6-phosphatase is controlled by compartmentalization. (1991)

Mouse L cells that possess the cation-independent mannose 6-phosphate (Man 6-P)/insulin-like growth factor (IGF) II receptor change the extent to which they dephosphorylate endocytosed acid hydrolases in response to serum (Einstein, R., and C. A. Gabel. 1989. J. Cell Biol. 109:1037-1046). To investigate the mechanism by which dephosphorylation competence is regulated, the dephosphorylation of individual acid hydrolases was studied in Man 6-P/IGF II receptor-positive and -deficient cell lines. 125I-labeled Man 6-P-containing acid hydrolases were proteolytically processed but remained phosphorylated when endocytosed by receptor-positive L cells maintained in the absence of serum; after the addition of serum, however, the cell-associated hydrolases were dephosphorylated. Individual hydrolases were dephosphorylated at distinct rates and to different extents. In contrast, the same hydrolases were dephosphorylated equally and completely after entry into Man 6-P/IGF II receptor-positive Chinese hamster ovary (CHO) cells. The dephosphorylation competence of Man 6-P/IGF II receptor-deficient mouse J774 cells was more limited. beta-Glucuronidase produced by these cells underwent a limited dephosphorylation in transit to lysosomes such that diphosphorylated oligosaccharides were converted to monophosphorylated species. The overall quantity of phosphorylated oligosaccharides associated with the enzyme, however, did not decrease within the lysosomal compartment. Likewise, beta-glucuronidase was not dephosphorylated when introduced into J774 cells via Fc receptor-mediated endocytosis. The CHO and J774 cell lysosomes, therefore, display opposite extremes with respect to their capacity to dephosphorylate acid hydrolases; within CHO cell lysosomes acid hydrolases are rapidly and efficiently dephosphorylated, but within J774 cell lysosomes the same acid hydrolases remain phosphorylated. This difference in processing indicates that lysosomes themselves exist in a dephosphorylation-competent and -incompetent state. Man 6-P-bearing acid hydrolases endocytosed by the L+ cells in the absence of serum were not distributed uniformly throughout the lysosomal compartment. The change in the dephosphorylation competence of L cells in response to serum suggests, therefore, that these cells contain multiple populations of lysosomes that differ with respect to their content of a mannose 6-phosphatase, and that serum factors affect the distribution of hydrolases between the different compartments.
INTERNET MESSAGE ACCESS PROTOCOL - VERSION 4rev1 (2003)

The Internet Message Access Protocol, Version 4rev1 (IMAP4rev1) allows a client to access and manipulate electronic mail messages on a server. IMAP4rev1 permits manipulation of mailboxes (remote message folders) in a way that is functionally equivalent to local folders. IMAP4rev1 also provides the capability for an offline client to resynchronize with the server.
THE REDUCTION OF NITRITES TO HYDROXYLAMINE BY SULFITES (1932)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTHE REDUCTION OF NITRITES TO HYDROXYLAMINE BY SULFITESG. K. Rollefson and C. F. OldershawCite this: J. Am. Chem. Soc. 1932, 54, 3, 977–979Publication Date (Print):March 1, 1932Publication History Published online1 May 2002Published inissue 1 March 1932https://pubs.acs.org/doi/10.1021/ja01342a019https://doi.org/10.1021/ja01342a019research-articleACS PublicationsRequest reuse permissionsArticle Views491Altmetric-Citations35LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Biomarkers in Natural Fish Populations Indicate Adverse Biological Effects of Offshore Oil Production (2011)

It is most remarkable to obtain biomarker responses in natural fish populations in the open sea that are similar to the biomarker responses in fish from highly polluted areas close to a point source. Risk assessment of various threats to the marine fish populations in the North Sea, such as overfishing, global warming, and eutrophication, should also take into account the ecologically relevant impact of offshore oil production.
Heme binding to murine erythroleukemia cells. Evidence for a heme receptor. (1985)

Friend virus transformed murine erythroleukemia (MEL) cells are known to take up heme from the surrounding medium and to incorporate it into newly synthesized hemoglobin (Granick, J. L., and Sassa, S. (1978) J. Biol. Chem. 253, 5402-5406), but the mechanism of its uptake is unknown. We hypothesized the existence of a specific receptor for heme in the plasma membrane. Using [55Fe]heme, we examined the characteristics of its interaction with MEL cells at 4 degrees C. [55Fe]heme binding reached equilibrium within 4 h, was 80% dissociable by 16 h, and was independent of pH over the range 7.0-8.2. Specific heme binding was linear with cell number, and competitive binding studies with various heme analogues, such as free protoporphyrin IX, metal-substituted protoporphyrin IX, Fe-mesoporphyrin IX, and Fe-deuteroporphyrin IX, revealed significant stereospecificity for Fe-protoporphyrin IX. The dissociation constant of the interaction was 0.03 nM-1 with no evidence of cooperativity or multiple classes of sites. The average number of sites/cell was approximately 10,300. Reduction of binding following preincubation with trypsin, in conjunction with the above data, suggests that this cell type may display a receptor for heme which is comprised, as least in part, of protein.
Application of PFBC's in rural locations (1993)

The high cost of providing power in remote regions of Alaska through the traditional use of diesel electric generator sets has prompted the Alaska Energy Authority (AEA) to evaluate Pressurized Fluidized-Bed Combustors (PFBC's) as a replacement technology. The Morgantown Energy Technology Center (METC) of the US Department of Energy (DOE) has been supplying AEA with data to further their analysis of the application of PFBC technology to power generation on village-scale systems. Starting with the results from studies managed by a review of how modular PFBC construction could be applied to remote villages was undertaken. Results of this study have applications to remote micro-grid village sites in Alaska as well as to off-grid located in other regions of the world.
Legal Issues in Medicine edited by Sheila McLean Aldershot: Gower Publishing Co Ltd 1981, xiv + 219 pp (hardback £13.50) (1982)

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Fingerprint biometric based Access Control and Classroom Attendance Management System (2015)

Fingerprint is a reliable biometric feature having a wide range of applications that require authentication. Person-specific verification is needed in many scenarios such as access-control, classroom attendance and financial transactions etc. In this paper, two applications of fingerprint biometric are proposed. An Access Control System (ACS) prototype is demonstrated for person-specific door access, using a fingerprinting device. Another prototype of a Classroom Attendance Management System (CAMS) is developed that uses fingerprint as biometric feature for classroom attendance. The CAMS consists of modules for database, web-user interface and views at multiple levels of access. Both systems are expected to mitigate the shortcomings of alternative existing systems, and eliminate the possibilities of spoofing or proxy. These systems store fingerprints along with the date/time-stamp for each user. Fingerprints are stored dynamically in a database for computing the different statistics, e.g., month-wise or semester-wise trends in the case of CAMS. The CAMS can also provide a solution to the problem of late-coming. Experiments are conducted for measuring the recognition accuracy, i.e., fingerprint match. The initial results of recognition accuracy at 87% for ACS and 92% for CAMS are encouraging. The proposed systems can be further scaled-up for real-time deployment, in applications such as employee attendance and controlled access to high-security areas etc.
Class I Histone Deacetylase-Selective Novel Synthetic Inhibitors Potently Inhibit Human Tumor Proliferation (2004)

We have developed previously a class of synthetic hybrid histone deacetylase (HDAC) inhibitors, which were built from hydroxamic acid of trichostatin A and pyridyl ring of MS-275. In this study we evaluated the antitumor effects of these novel hybrid synthetic HDAC inhibitors, SK-7041 and SK-7068, on human cancer cells. Both SK-7041 and SK-7068 effectively inhibited cellular HDAC activity at nanomolar concentrations and induced the time-dependent hyperacetylation of histones H3 and H4. These HDAC inhibitors preferentially inhibited the enzymatic activities of HDAC1 and HDAC2, as compared with the other HDAC isotypes, indicating that class I HDAC is the major target of SK-7041 and SK-7068. We found that these compounds exhibited potent antiproliferative activity against various human cancer cells in vitro. Growth inhibition effect of SK-7041 and SK-7068 was related with the induction of aberrant mitosis and apoptosis in human gastric cancer cells. Both compounds induced the accumulation of cells at mitosis after 6 h of treatment, which was demonstrated by accumulation of tetraploid cells, lack of G(2) cyclin/cyclin-dependent kinase inactivation, and higher mitotic index. After 12 h of treatment, apoptotic cells were increased through mitochondrial and caspase-mediated pathway. Finally, in vivo experiment showed that SK-7041 or SK-7068 was found to reduce the growth of implanted human tumors in nude mice. Therefore, based on isotype specificity and antitumor activity, SK-7041 and SK-7068 HDAC inhibitors are expected to be promising anticancer therapeutic agents and need additional clinical development.
Fluorescence-Linked Immunosorbent Assay (FLISA) for Quantification of Antibodies to Food Antigens (1987)

Serum antibodies of the IgG type from rabbits immunized with food antigens (beta-lactoglobulin, ovalbumin and gliadin) have been quantified using a fluorescence-linked immunosorbent assay (FLISA), with the antigens adsorbed as round spots (about 8 mm in diameter) on glass or plastic microscope slides. The indirect immunofluorescence intensities were determined using a microscope fluorometer, and compared to enzyme-linked immunosorbent assay (ELISA) in microtiter plates and diffusion-in-gel-ELISA (DIG-ELISA) in plastic petri dishes. It was found that FLISA in general became more sensitive when the antigens had been adsorbed onto a plastic (Nunclon) than onto a glass surface. When the antigens were adsorbed to the plastic slides, the relative sensitivity order (maximum serum dilution) of the assays was in general the following, ELISA greater than FLISA greater than DIG-ELISA. The fluorescence-linked method appeared to require equal or less antigen and conjugated antiserum per sample. Due to the visual inspection of the surface, inhomogeneities of the antigen-coating could be readily discovered and evaluated by several measurements within the field of antigen-antibody reaction. It is proposed that spot FLISA may be an alternative to ELISA especially when the amount of antigen or antiserum is limited.
StoArranger: Enabling Efficient Usage of Cloud Storage Services on Mobile Devices (2017)

Cloud storage usages are becoming increasingly popular on mobile devices. Through an extensive motivation study, we find that cloud storage accesses from mobile apps suffer from several notable problems that undermine usage experiences. The root cause is that the way of cloud storage providers deploying their services onto mobile devices relies on app developers for the correct and appropriate implementations and lacks the ability of monitoring and servicing client-side cloud storage accesses. We propose StoArranger, a practical system framework that solves the problems by coordinating, rearranging, and transforming cloud storage communications on mobile devices. We have prototyped the proposed system using two different implementation approaches. We discuss our experiences of the implementations in the paper. The real-app evaluation experiments show that StoArranger can significantly improve mobile cloud storage access efficiency with little overheads.
110-GB/s simultaneous bidirectional transceiver logic synchronized with a system clock (1999)

A simultaneous bidirectional transceiver logic (SBTL), for a 0.25 /spl mu/m CMOS embedded array, has a low-voltage-swing input flip-flop circuit and an output flip-flop with a boundary scan to enable a 1.1-Gb/s data transfer per LSI pin with a 550-MHz system clock. Clock skew and jitter minimization enables high bandwidth in a phase-locked system. Measured latency time for transmission is less than 3.0 ns during simultaneous switching mode when the cable length is 18 cm. Average power consumption is 12 mW per pin at 550 MHz. A low-noise output buffer and a controlled collapse chip connection (C4)-based 1595-pin package with on-package capacitors achieve 100-byte data bus. The maximum data bandwidth per LSI is 110 GB/s.
Expression, Purification and Characterization of Soluble Recombinant Periostin Protein Produced by Escherichia coli (2009)

Periostin is a matricellular protein participating in the tissue remodelling of damaged cardiac tissue after acute myocardial infarction and of the periodontal ligament in mice. However, further studies on the periostin protein have been limited by the intrinsic difficulty of purifying this protein produced in Escherichia coli due to its insolubility. Here, we demonstrate the expression of recombinant periostin protein with high solubility and monodispersity in E. coli. Periostin is composed of an amino-terminal EMI domain, a tandem repeat of 4 fas1 domains (RD1-4), and a carboxyl-terminal region (CTR). We expressed the RD4-CTR region tagged with GST at amino-terminal and 6x Histidine at carboxyl-terminal end in E. coli. The recombinant protein was purified by using GSH-Sepharose and nickel chelation affinity chromatography, followed by gel filtration chromatography. The RD4-CTR protein exhibited high solubility and monodispersity. On average, 9.1 mg of purified RD4-CTR was routinely obtained from 1 L of culture media. Furthermore, the RD4-CTR was biochemically active, because it bound to the RD1-4, the same as intact periostin protein that had been purified from mammalian cells. Our results should enable us to produce the periostin recombinant protein in large quantities and facilitate future studies on functional and structural analyses of periostin.
Stability of Photo-Induced Alignment of Azobenzene-Containing Polyimides (2005)

ABSTRACT We have examined the change in the molecular orientation of the photo-aligned film of polyimide containing azobenzene in the backbone structure (Azo-PI) by a washing treatment. The Azo-PI films were washed in 2-propanol with an ultrasonic cleaner for 5 min and then in pure water for 5 min. No noticeable change was observed in the in-plane molecular orientation of the photo-aligned Azo-PI films. On the other hand, the in-plane molecular orientation of a rubbed polyimide (poly[4,4′-oxydiphenylene-1,2,3,4-cyclobutanetetracarboximide]) film was relaxed by washing. From these results we conclude that the molecular orientation of the photo-aligned Azo-PI films is more stable than that of the rubbed polyimide film.