gebhart/patrepeval huggingface dataset:
A novel spin-labeled amino acid derivative for use in peptide synthesis: (9-fluorenylmethyloxycarbonyl)-2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid (1993)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA novel spin-labeled amino acid derivative for use in peptide synthesis: (9-fluorenylmethyloxycarbonyl)-2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acidReinaldo Marchetto, Shirley Schreier, and Clovis R. NakaieCite this: J. Am. Chem. Soc. 1993, 115, 23, 11042–11043Publication Date (Print):November 1, 1993Publication History Published online1 May 2002Published inissue 1 November 1993https://pubs.acs.org/doi/10.1021/ja00076a093https://doi.org/10.1021/ja00076a093research-articleACS PublicationsRequest reuse permissionsArticle Views782Altmetric-Citations139LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
The Upper Limit of Cerebral Blood Flow Autoregulation in Acute Intracranial Hypertension (1998)

The present series of experiments was performed to investigate the influence of acute intracranial hypertension on the upper limit (UL) of cerebral blood flow (CBF) autoregulation. Three groups of eight rats each--one with normal intracranial pressure (ICP) (2 mmHg), one with ICP = 30 mmHg, and one with ICP = 50 mmHg--were investigated. Intracranial hypertension was maintained by continuous infusion of lactated Ringer's solution into the cisterna magna, where the pressure was used as ICP. Cerebral perfusion pressure (CPP), calculated as mean arterial blood pressure (MABP)-ICP, was increased stepwise by continuous intravenous infusion of norepinephrine. CBF was calculated by the intracarotid 133Xe method. In all three groups the corresponding CBF/CPP curve included a plateau where CBF was independent of changes in CPP, showing intact autoregulation. At normal ICP the UL was found at a CPP of 141 +/-2 mmHg, at ICP = 30 mmHg the UL was 103+/-5 mmHg, and at ICP = 50 mmHg the UL was found at 88+/-7 mmHg. This shift of the UL was more pronounced than the shift of the lower limit (LL) of the CBF autoregulation found previously. We conclude that intracranial hypertension is followed by both a shift toward lower CPP values and a narrowing of the autoregulated interval between the LL and the UL.
Redox‐Responsive Self‐Healing for Corrosion Protection (2013)

Raspberry-shaped redox-responsive capsules for storing corrosion inhibitors are introduced, targeted to solve the drawbacks of conducting-polymer-based coating systems for corrosion protection. These capsules synthesized via the miniemulsion technique have a remarkable release property upon reduction (onset of corrosion) and cease release upon reoxidation (passivation of the defect). The self-healing capability is demonstrated by application of these capsules as part of a composite coating on zinc.
Plexiform angiomyxoid myofibroblastic tumour: differential diagnosis of gastrointestinal stromal tumour in the stomach (2008)

Mesenchymal tumours other than gastrointestinal stromal tumours are rare in the stomach. Nevertheless it is important to incorporate them into the differential diagnosis. Plexiform angiomyxoid myofibroblastic tumour is a recently described new entity of a presumably benign mesenchymal gastric tumour. This report presents what is believed to be the third case of this tumour. The tumour is characterised by bland spindle cells in a plexiform pattern, a mucinous extracellular matrix and a network of thin blood vessels. These findings are completely in line with the two previous reported cases. There was a strong positivity for alpha-smooth muscle actin and a low proliferation index (<2%). The tumour had no C-KIT or CD34 expression and no mutation in the C-KIT and PDFGRalpha genes. Plexiform angiomyxoid myofibroblastic tumour may present a new mesenchymal tumour entity in the stomach.
Structural comparison of four different antibodies interacting with human papillomavirus 16 and mechanisms of neutralization (2015)

Cryo-electron microscopy (cryo-EM) was used to solve the structures of human papillomavirus type 16 (HPV16) complexed with fragments of antibody (Fab) from three different neutralizing monoclonals (mAbs): H16.1A, H16.14J, and H263.A2. The structure-function analysis revealed predominantly monovalent binding of each Fab with capsid interactions that involved multiple loops from symmetry related copies of the major capsid protein. The residues identified in each Fab-virus interface map to a conformational groove on the surface of the capsomer. In addition to the known involvement of the FG and HI loops, the DE loop was also found to constitute the core of each epitope. Surprisingly, the epitope mapping also identified minor contributions by EF and BC loops. Complementary immunological assays included mAb and Fab neutralization. The specific binding characteristics of mAbs correlated with different neutralizing behaviors in pre- and post-attachment neutralization assays.
Experiences with the Alternate Reality Kit: An Example of the Tension between Literalism and Magic (1987)

This article presents an overview of the Alternate Reality Kit, an animated environment for creating interactive simulations. ARK is built upon a physical-world metaphor: All objects have an image, a position, a velocity, and the ability to experience forces. Users manipulate objects with a mouse-operated "hand," which enables them to carry and throw objects, press buttons, and operate sliders. The interface features are discussed in light of a general user interface tension between literalism and magic. Literal features-defined as those that are true to the interface's metaphor-enhance an interface's learnability. Magical features are defined as those capabilities that deliberately violate the metaphor in order to provide enhanced functionality. Discussion of each ARK feature includes informal observations of early ARK users, and an assessment of the feature's learnability, its usefulness, and its position on the magical-literal axis. Despite ARK's magical features, applications-level users have been trained in a few minutes. Although this article is about ARK, the tension between literalism and magic raises some interesting questions on its own. Some of these questions are presented briefly in the conclusion.
Antitumor activity of SPM VIII, a derivative of the nucleoside antibiotic spicamycin, against human tumor xenografts. (1994)

The antitumor activity of spicamycin analogue SPM VIII against human stomach, breast, lung, colon and esophageal cancers was compared to that of mitomycin C (MMC) in the human tumor-nude mice xenograft model. Comparative studies of SPM VIII given i.v. at 6 mg/kg/day daily for 5 days and MMC given i.v. at 6.7 mg/kg on day 1 revealed that the antitumor spectrum of SPM VIII showed a different pattern from that of MMC and that SPM VIII caused tumor mass reductions in more tumors than did MMC in colon cancers (4/12 versus 1/11). In addition to this study, a comparative study of SPM VIII given i.v. at 12 mg/kg/day 8 times at 3- or 4-day intervals and 5'-deoxy-5-fluorouridine (5'-DFUR) given po at 185 mg/kg/day 5 days per week for 4 weeks showed that SPM VIII had the highest effect on SC-9 human stomach cancer and COL-1 human colon cancer among the 3 compounds, resulting in a significant reduction of tumor mass. Although other pharmacological studies are in progress, these results suggest that SPM VIII might be a novel antitumor compound effective for human cancers including cancer of the digestive organs.
Phonetic recognition in a segment-based HMM (1993)

The author describes a segment-based HMM (hidden Markov model) recognizer and presents phonetic recognition results achieved with the system. As opposed to a conventional frame-based HMM, measurements in such a system are made on variable-duration segments. The key experimental result is that inclusion of measurements made beyond segment boundaries improves phonetic recognition performance significantly. On a set of nine male test speakers from the VOYAGER corpus, the system obtained a phonetic recognition accuracy of 59% (95% confidence interval of 53-65%) on a 39-class phonetic recognition task. Although little attempt was made to optimize system parameters, this result is competitive with existing systems of comparable complexity.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The Effects of Maternally Administered Methadone, Buprenorphine and Naltrexone on Offspring: Review of Human and Animal Data (2008)

Most women using heroin are of reproductive age with major risks for their infants. We review clinical and experimental data on fetal, neonatal and postnatal complications associated with methadone, the current "gold standard", and compare these with more recent, but limited, data on developmental effects of buprenorphine, and naltrexone. Methadone is a micro-opioid receptor agonist and is commonly recommended for treatment of opioid dependence during pregnancy. However, it has undesired outcomes including neonatal abstinence syndrome (NAS). Animal studies also indicate detrimental effects on growth, behaviour, neuroanatomy and biochemistry, and increased perinatal mortality. Buprenorphine is a partial micro-opioid receptor agonist and a kappa-opioid receptor antagonist. Clinical observations suggest that buprenorphine during pregnancy is similar to methadone on developmental measures but is potentially superior in reducing the incidence and prognosis of NAS. However, small animal studies demonstrate that low doses of buprenorphine during pregnancy and lactation lead to changes in offspring behaviour, neuroanatomy and biochemistry. Naltrexone is a non-selective opioid receptor antagonist. Although data are limited, humans treated with oral or sustained-release implantable naltrexone suggest outcomes potentially superior to those with methadone or buprenorphine. However, animal studies using oral or injectable naltrexone have shown developmental changes following exposure during pregnancy and lactation, raising concerns about its use in humans. Animal studies using chronic exposure, equivalent to clinical depot formulations, are required to evaluate safety. While each treatment is likely to have maternal advantages and disadvantages, studies are urgently required to determine which is optimal for offspring in the short and long term.
Fullerene doped glasses (1994)

Optical glasses doped with a fullerene mixture of C60/C70 were made in our lab. Three peaks, corresponding to C60, C70, and a modified fullerene (possibly its oxide), respectively, were found in the chromatogram of high performance liquid chromatography (HPLC) analysis of the 0.1-wt % fullerene doped phosphate glass. Depending on the doping procedure and impurity, weak to strong interacting between C60 and its environment was found by the nuclear magnetic resonance study. For both phosphate and fluorophosphate glasses with 0.01-wt % fullerene mixture doping, the eigen UV absorption edge showed a significant blueshift, indicating that the bonding between the nonbridging oxygen in these glasses was subjected to a strong change due to the fullerene doping.
Motivation for Contemporary Tattoo Removal (2008)

In both the 1996 and the 2006 studies, a shift in identity occurred, and removal centered around dissociating from the past. However, in the 2006 study, more women than men were notably affected by possession risks. Societal support for women with tattoos may not be as strong as for men. Rather than having visible tattoos, women may still want to choose self-controlled body site placement, even in our contemporary society.
Pepsin−Gold Colloid Conjugates:  Preparation, Characterization, and Enzymatic Activity (2001)

Pepsin−colloidal gold conjugates were prepared by a simple protein-friendly process and the enzymatic activity of the bioconjugates is reported. The pepsin−gold conjugates are obtained by mixing colloidal gold and protein solutions at pH = 3 and, thereafter, centrifugation, washing, and redispersion of the pepsin−gold conjugate material in water. The bioconjugates in solution were characterized by UV−vis spectroscopy, fluorescence spectroscopy, and biocatalytic activity measurements while films of the bioconjugate material obtained by solvent evaporation on suitable substrates were further analyzed by scanning electron microscopy (SEM), energy dispersive analysis of X-rays (EDAX), transmission electron spectroscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). While TEM and SEM measurements showed aggregates of the enzyme/colloidal gold conjugates, the intactness of secondary and tertiary structures of the enzyme, as determined by FTIR and fluorescence spectroscopies and confirmed by biocatalytic activity measurements, clearly indicates that the enzyme is stable in its natural state and is possibly stabilized by the colloidal gold particles. The enzyme in the pepsin−Au bioconjugate retained substantial biocatalytic activity and was more stable than the free enzyme in solution.
High-Throughput Cancer Cell Sphere Formation for Characterizing the Efficacy of Photo Dynamic Therapy in 3D Cell Cultures (2015)

Photodynamic therapy (PDT), wherein light sensitive non-toxic agents are locally and selectively activated using light, has emerged as an appealing alternative to traditional cancer chemotherapy. Yet to date, PDT efficacy has been mostly characterized using 2D cultures. Compared to 2D cultures, 3D sphere culture generates unique spatial distributions of nutrients and oxygen for the cells that better mimics the in-vivo conditions. Using a novel polyHEMA (non-adherent polymer) fabrication process, we developed a microfluidic sphere formation platform that can (1) generate 1,024 uniform (size variation <10%) cancer spheres within a 2 cm by 2 cm core area, (2) culture spheres for more than 2 weeks, and (3) allow the retrieval of spheres. Using the presented platform, we have successfully characterized the different responses in 2D and 3D cell culture to PDT. Furthermore, we investigated the treatment resistance effect in cancer cells induced by tumor associated fibroblasts (CAF). Although the CAFs can enhance the resistance to traditional chemotherapy agents, no significant difference in PDT was observed. The preliminary results suggest that the PDT can be an attractive alternative cancer therapy, which is less affected by the therapeutic resistance induced by cancer associated cells.
5‐HT<sub>1A</sub>receptors are involved in the cannabidiol‐induced attenuation of behavioural and cardiovascular responses to acute restraint stress in rats (2008)

The results suggest that CBD can attenuate acute autonomic responses to stress and its delayed emotional consequences by facilitating 5-HT(1A) receptor-mediated neurotransmission.
The SpiNNaker Project (2014)

The spiking neural network architecture (SpiNNaker) project aims to deliver a massively parallel million-core computer whose interconnect architecture is inspired by the connectivity characteristics of the mammalian brain, and which is suited to the modeling of large-scale spiking neural networks in biological real time. Specifically, the interconnect allows the transmission of a very large number of very small data packets, each conveying explicitly the source, and implicitly the time, of a single neural action potential or “spike.” In this paper, we review the current state of the project, which has already delivered systems with up to 2500 processors, and present the real-time event-driven programming model that supports flexible access to the resources of the machine and has enabled its use by a wide range of collaborators around the world.
Solubilization of rat lung vasoactive intestinal peptide receptors in the active state. Characterization of the binding properties and comparison with membrane-bound receptors. (1988)

We demonstrate here that rat lung membrane vasoactive intestinal peptide (VIP) receptors can be extracted in the active state using digitonin. Sepharose 4B gel filtration chromatography was utilized to demonstrate the formation of specific binding complexes between 125I-VIP and solubilized receptors. A rapid soluble receptor assay was established to separate 125I-VIP-receptor complexes from free 125I-VIP, which entailed differential precipitation of the 125I-VIP-receptor complex with polyethylene glycol and bovine gamma-globulin. Using this assay, several detergents were tested for their suitability to extract active VIP receptors, and most favorable results were obtained with digitonin, as judged by specific binding of 125I-VIP to the solubilized receptors. Time course studies indicated that the binding of 125I-VIP to digitonin extract was more rapid than to rat lung membranes. Scatchard analyses of competitive binding data indicated the presence of two classes of binding sites in the digitonin extract, as in the membrane. The values for the dissociation constants (Kd) were 200 pM for Class I and 8 nM for Class II receptors while the values for binding capacity (Bmax) were 200 and 2300 fmol/mg for Class I and II sites, respectively. Although the binding parameters of the two classes were similar to those in the membrane, the pharmacological properties were different, as evidenced by the inability of rat growth hormone releasing factor, a potent VIP agonist in the membrane, to displace specifically bound 125I-VIP from solubilized receptors. The ability to solubilize active VIP receptors represents an important step toward purification of the functional protein.
A 2-nW 1.1-V self-biased current reference in CMOS technology (2005)

This work presents the design of an ultra-low-power self-biased 400-pA current source. We propose the use of a very simple topology along with a design methodology based on the concept of inversion level. An efficient design methodology has resulted in a cell area around 0.045 mm/sup 2/ in the AMI 1.5-/spl mu/m CMOS technology and power consumption around 2 nW for 1.2-V supply. Simulated and experimental results validate the design and show that the current source can operate at supply voltages down to 1.1 V with a good regulation (<6% /V variation of the supply voltage) in a 1.5-/spl mu/m technology.
Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone (2001)

We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histones, preferentially to histone H3, to mediate formation of nucleosome, and to decondense sperm chromatin. These activities of B23 were dependent on its acidic regions as other histone chaperones, suggesting that B23/nucleophosmin is a member of histone chaperone proteins.
In re Visbeck. (1986)

The Superior Court of New Jersey ordered the appointment of a guardian to consent to implantation of a feeding tube for a 90-year-old patient who had suffered a severely disabling stroke, though her son had not agreed to the proposed procedure. The court reasoned that a patient who becomes incompetent does not lose the right to accept or refuse medical treatment; instead, a substitute or surrogate decision maker exercises the patient's right of consent. Without the surgical implantation of a feeding tube in the patient's stomach, she would have suffered death from dehydration and starvation.
Expression of two different forms of fibroblast growth factor receptor 1 in different mouse tissues and cell lines. (1991)

The fibroblast growth factor receptors (FGFRs) form a multigene family of at least four members, all having extracellular regions consisting of either two or three immunoglobin-like (Ig-like) domains. By RNase protection analysis we have analyzed the expression of FGFR-1 mRNA in various tissues and cell lines and demonstrated that all of the cell lines studied expressed at least two different forms of the FGFR-1 at similar levels. Although muscle and heart express forms having either two [FGFR-1 short (FGFR-1S)] or three [FGFR-1 long (FGFR-1L)] Ig-like domains, the developing brain and adult brain express only mRNA encoding the longer form. The two forms of the receptor were characterized further by stably introducing expression vectors expressing them into Rat-2 fibroblasts and FDC-P1 myeloid cells. Treatment of the transfected Rat-2 cells with acidic FGF (aFGF) or basic FGF (bFGF) resulted in focus formation. The transformed phenotype was observed even without addition of ligand after growth in culture for greater than 2 months. Cross-linking of 125I-labeled bFGF (125I-bFGF) to Rat-2 cells expressing either FGFR-1L or FGFR-1S yielded two similar complexes of 150 and 110 kDa. Although Rat-2 cells expressing FGFR-1L yielded similar complexes with 125I-labeled aFGF (125I-aFGF), only the 150-kDa complex was formed with cells expressing FGFR-1S. The 150-kDa complex was also observed when 125I-aFGF or 125I-bFGF was cross-linked to FDC-P1 cells expressing FGFR-1L. Significantly, these complexes were only observed when heparin was present in the cross-linking reaction. FDC-P1 cells expressing FGFR-1 bound aFGF and bFGF with high affinity but only in the presence of heparin. The factor dependence of these cells could be switched from interleukin 3 to FGF in the presence of heparin.
Doping Effect of Tetrathianaphthacene Molecule in Organic Semiconductors on Their Interfacial Electronic Structures Studied by UV Photoemission Spectroscopy (2005)

The n-type doping ability of the electron-donating organic molecule tetrathianaphthacene (TTN) was examined by ultraviolet photoemission spectroscopy (UPS) for two kinds of matrix organic semiconductors, hexadecafluorophthalocyaninatozinc (F 16 ZnPc) and tris(8-hydroxyquinoline)aluminum (Alq 3 ). The observed dependence of the UPS spectra suggests that TTN acts as a good donor in F 16 ZnPc, while the effect was not so significant for Alq 3 . These results and the consideration of the energy parameters of these molecules indicate that a close match between the highest occupied molecular orbital of the dopant and the lowest unoccupied molecular orbital of the matrix is important for efficient n-type doping.
Signal fluctuations due to individual droplets in inductively coupled plasma atomic emission spectrometry (1989)

Large emission intensity fluctuations are observed from analyte species in inductively coupled plasmas. Relative standard deviations are as large as 71% when emission is viewed with time resolution of 10 microseconds. Low in the plasma, peaks in atom emission intensity are accompanied by depressions in ion emission. This behavior appears to be due to local cooling by aerosol droplets. High in the plasma, peaks in atom emission are followed by peaks in ion emission. These emission spikes result from atomization and ionization of analyte from vaporizing particles. Laser light scattering experiments show that droplets or particles exist in a conventional 1.0-kW plasma up to 20 mm above the load coil. Emission signals detected high in the plasma correlate with laser light scattering signals below.