gebhart/patrepeval huggingface dataset:
Enhancement of Endotoxicity and Reactivity with Carbocyanine Dye by Sonication of Lipopolysaccharide (1984)

The specificity of endotoxin (lipopolysaccharide, LPS) in the carbocyanine dye reaction was investigated, and then a stoichiometric study of the dye-LPS interaction was conducted with attention to the relationship of biological activities of LPS to the reactivity with the dye. Absorption maxima of some bacterial components in the dye reaction were as follows; LPS from both Escherichia coli and Pseudomonas aeruginosa and lipid A from E. coli LPS, 465 nm; Shigella flexneri LPS, 460 nm; Salmonella minnesota R595 glycolipid, 470 nm; polysaccharide from E. coli LPS, 650 nm; yeast RNA, 620 nm; streptococcal M protein and pyrogenic exotoxin, 610 nm; and free fatty acids, 445-450 nm. The absorbance at 465 nm was increased approximately threefold by sonicating LPS for 1-3 min, which roughly paralleled the decrease in turbidity of the LPS aqueous solution. The Limulus amoebocyte lysate (LAL) gelation activity of LPS increased 10-fold when LPS was sonicated for 0.5-5 min, but it decreased to the control level after further treatment. This decrease, however, was overcome by sonication in the presence of 5 mmol of L-ascorbic acid used as an antioxidant. The LAL gelation activity of LPS was inactivated in parallel with an increase in the ratio (w/w) of dye to LPS from 1.73 to 6.90 in the dye-LPS mixture. Pyrogenicity of LPS was also clearly inactivated when the ratio was over 1.73. The ratios of the height of the beta band at 465 nm (dye-LPS complex) to that of the alpha band at 510 nm (free dye) were increased by sonicating LPS, indicating that the binding character, or stacking tendency, was increased by sonicating LPS.
Binding of the Vesicle Docking Protein p115 to Golgi Membranes Is Inhibited under Mitotic Conditions (1996)

The vesicle docking protein p115 showed saturable, high affinity binding to interphase Golgi membranes. The affinity of binding was up to 20-fold lower using membranes preincubated with mitotic cytosol. In contrast, binding was not affected by mitotic pretreatment of p115. The reduction in p115 binding was mediated by phosphorylation, could be induced by a cyclin-dependent kinase, and was fully reversible. A shift of p115 from membranes to cytosol was also found after fractionating mitotic cells. The functional significance of the decreased binding was addressed by in vitro mitotic incubations which disassemble Golgi cisternae, predominantly producing transport vesicles. The addition of excess p115 decreased loss of membrane from cisternae, indicating that p115's action is limiting while transport vesicles accumulate. The cessation of intra-Golgi traffic in mitosis has been hypothesized to result from an inhibition of membrane fusion while budding of transport vesicles continues. This process also contributes to mitotic Golgi disassembly. Our results imply that there is a mitotic modification to Golgi membranes leading to a reduction in the affinity of the p115 receptor. Reduced p115 binding may play a part in the inhibition of membrane fusion by preventing prior vesicle docking.
Enzymatic Cross-Linking of Resilin-Based Proteins for Vascular Tissue Engineering Applications (2016)

Protein-based biomaterials have received significant attention for tissue engineering applications. For example, resilin-based protein gels have been produced with different cross-linking chemistries for applications in cartilage, cardiovascular, and vocal fold engineering. In this study, we investigate an alternative cross-linking chemistry to form resilin-based protein hydrogels and demonstrate the versatility of the gels for investigating cell response to matrix stiffness. Specifically, transglutaminase was used to cross-link proteins and resulted in gel surfaces more suitable for long-term cell attachment compared to those formed by a Mannich-type condensation reaction. Since matrix stiffness is an important determinant in modulating cell response, we first tuned matrix stiffness by varying total protein concentration. Next, we observed that matrix stiffness modulated cell spreading and endothelial differentiation of human mesenchymal stem cells. In particular, our results show that cells differentiated on our matrices, which have a stiffness similar to subendothelial layers, had statistically equivalent endothelial function compared to cells differentiated on hard glass surfaces. Thus, our protein-based matrix system is a promising tool to provide substrates favorable for long-term cell attachment and better mimics the native subendothelial environment compared to conventional hard culture substrates.
Highly Sensitive Glucose Sensor Based on Pt Nanoparticle/Polyaniline Hydrogel Heterostructures (2013)

Glucose enzyme biosensors have been shown useful for a range of applications from medical diagnosis, bioprocess monitoring, to beverage industry and environmental monitoring. We present here a highly sensitive glucose enzyme sensor based on Pt nanoparticles (PtNPs)-polyaniline (PAni) hydrogel heterostructures. High-density PtNPs were homogeneously loaded onto the three-dimensional (3D) nanostructured matrix of the PAni hydrogel. The PtNP/PAni hydrogel heterostructure-based glucose sensor synergizes the advantages of both the conducting hydrogel and the nanoparticle catalyst. The porous structure of the PAni hydrogel favored the high density immobilization of the enzyme and the penetration of water-soluble molecules, which helped efficiently catalyze the oxidation of glucose. In addition, the PtNPs catalyzed the decomposition of hydrogen peroxide that was generated during the enzymatic reaction. The transferred charges from these electrochemical processes were efficiently collected by the highly conducting PtNP/PAni hydrogel heterostructures. The glucose enzyme sensor based on this heterostructure exhibited unprecedented sensitivity, as high as 96.1 μA·mM(-1)·cm(-2), with a response time as fast as 3 s, a linear range of 0.01 to 8 mM, and a low detection limit of 0.7 μM.
Preparation and Assay of a Red Cell Fraction of the Rh Factor (1951)

Journal Article Preparation and Assay of a Red Cell Fraction of the Rh Factor Get access Bettina B. Carter, M.S. Bettina B. Carter, M.S. From the Inslilute of Pathology, Western Pennsylvania Hospital, Pittsburgh, Pennsylvania Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 21, Issue 6, 1 June 1951, Pages 561–565, https://doi.org/10.1093/ajcp/21.6.561 Published: 01 June 1951 Article history Received: 06 November 1950 Published: 01 June 1951
ChemInform Abstract: A New and Versatile Synthesis of 5‐Substituted Pyrrolo[2,3‐d]pyrimidines. (2001)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Using Lean-Based Systems Engineering to Increase Capacity in the Emergency Department (2014)

In this single center trial, a focused Lean-based reorganization of patient flow improved Fast Track ED performance measures and capacity, without added expense. Broad multi-centered application of systems engineering science might further improve ED throughput and capacity.
Tracking and Recognising Hand Gestures using Statistical Shape Models. (1995)

Hand gesture recognition from video images is of considerable interest as a means of providing simple and intuitive man-machine interfaces. Possible applications range from replacing the mouse as a pointing device to virtual reality and communication with the deaf. We describe an approach to tracking a hand in an image sequence and recognising, in each video frame, which of five gestures it has adopted. A statistically based Point Distribution Model (PDM) is used to provide a compact parameterised description of the shape of the hand for any of the gestures or the transitions between them. The values of the resulting shape parameters are used in a statistical classifier to identify gestures. The model can be used as a deformable template to track a hand through a video sequence but this proves unreliable. We describe how a set of models, one for each of the five gestures, can be used for tracking with the appropriate model selected automatically. We shown that this results in reliable tracking and gesture recognition for two 'unseen' video sequences in which all the gestures are used.
Advances in Content Management and Protection (1999)

The need for content management and protection has resulted in significant progress being made in this area for a flexible, low cost open system that is applicable to a wide range of consumer electronics equipment. — Examples are presented that show how the new Content Management and Protection Technology can be applied to: • DVD Disks • Digital TV Broadcasting • CD Games • Electronic Banking • Internet Content Delivery
Peptidergic Nociceptors of Both Trigeminal and Dorsal Root Ganglia Express Serotonin 1D Receptors: Implications for the Selective Antimigraine Action of Triptans (2003)

Agonists at serotonin 1D (5-HT1D) receptors relieve migraine headache but are not clinically used as general analgesics. One possible explanation for this difference is that 5-HT1D receptors are preferentially expressed by cranial afferents of the trigeminal system. We compared the distribution of 5-HT1D receptor-immunoreactive (5-HT1D-IR) peripheral afferents within the trigeminal ganglion (TRG) and lumbar dorsal root ganglion (DRG) of the rat. We also examined the neurochemical identity of 5-HT1D-IR neurons with markers of primary afferent nociceptors, peripherin, isolectin B4, and substance P, and markers of myelinated afferents, N52 and SSEA3. We observed a striking similarity in the size, distribution, and neurochemical identity of 5-HT1D-IR neurons in TRG and lumbar DRG afferents. Furthermore, the vast majority of 5-HT1D-IR neurons are unmyelinated peptidergic afferents that distribute peripherally, including the dura, cornea, and the sciatic nerve. In the central projections of these afferents within the trigeminal nucleus caudalis and the spinal cord dorsal horn, 5-HT1D-IR fibers are concentrated in laminas I and outer II; a few axons penetrate to lamina V. At the ultrastructural level, 5-HT1D receptors in the spinal cord dorsal horn are localized exclusively within dense core vesicles of synaptic terminals. We observed scattered 5-HT1D-IR neurons in the nodose ganglia, and there was sparse terminal immunoreactivity in the solitary nucleus. The visceral efferents of the superior cervical ganglia did not contain 5-HT1D immunoreactivity. Our finding, that 5-HT1D receptors are distributed in nociceptors throughout the body, raises the possibility that triptans can regulate not only headache-associated pain but also nociceptive responses in extracranial tissues.
HBase fine grained access control with extended permissions and inheritable roles (2015)

HBase is a widely used distributed and column-oriented database based on HADOOP and HDFS. But it still has some shortcomings in storing and sharing data. In order to upgrade the security level of HBase, this paper proposes a fine-grained access control framework which extends the access control permissions according to the atomic operations of HBase. Meanwhile, we use XACML for permissions implementation which provides RBAC and Role-Inheritance and guarantees permissions management more conveniently. From the experiment, it shows that the fine grained access control for HBase is practical and the increased overload can almost be ignored while executing the permissions matching.
Imaging Properties of a Series of Magnetic Electron Lenses (1951)

The paraxial lens data and the more important first order lens aberrations were determined for a series of symmetrical magnetic lenses of equal lens diameter D but variable air gap width S for a wide range of lens excitations. The range of gap widths covered was S/D=0.2 to S/D=2 The field distribution within the lenses was measured to a high degree of accuracy with a resistance network analogue, and the lens data were then obtained through numerical trajectory tracing. The results are represented as a series of graphs These results are representative of the most common magnetic electron lenses, and should have useful applications in lens design. An approximate formula for the focal length is fapproximately equal to50(DS)1/2Vr/(NI)2. A few design examples are discussed.
Hormone concentrations in synovial fluid of patients with rheumatoid arthritis. (2005)

Besides the steroids also insulin, c-peptide, GH and FT3 were found in synovial fluid. The elevated ALD and GH levels in synovial fluid of RA patients and the presence of INS in synovial fluid with increase of INS binding to plasma membranes of cells from synovial fluid of RA patients suggest that besides the gonadal steroids also these hormones may affect the local inflammatory processes.
The Role of Endothelial Nitric Oxide Synthase in the Cerebral Hemodynamics after Controlled Cortical Impact Injury in Mice (2003)

Traumatic brain injury causes a reduction in cerebral blood flow, which may cause additional damage to the brain. The purpose of this study was to examine the role of nitric oxide produced by endothelial nitric oxide synthase (eNOS) in these vascular effects of trauma. To accomplish this, cerebral hemodynamics were monitored in mice deficient in eNOS and wild-type control mice that underwent lateral controlled cortical impact injury followed by administration of either L-arginine, 300 mg/kg, or saline at 5 min after the impact injury. The eNOS deficient mice had a greater reduction in laser Doppler flow (LDF) in the contused brain tissue at the impact site after injury, despite maintaining a higher blood pressure. L-Arginine administration increased LDF post-injury only in the wild-type mice. L-Arginine administration also resulted in a reduction in contusion volume, from 2.4 ± 1.5 to 1.1 ± 1.2 mm3 in wild-type mice. Contusion volume in the eNOS deficient mice was not significantly altered by L-arginine administration. These differences in cerebral hemodynamics between the eNOS-deficient and the wild-type mice suggest an important role for nitric oxide produced by eNOS in the preservation of cerebral blood flow in contused brain following traumatic injury, and in the improvement in cerebral blood flow with L-arginine administration.
Evolutionary, Structural and Biochemical Evidence for a New Interaction Site of the Leptin Obesity Protein (2003)

The Leptin protein is central to the regulation of energy metabolism in mammals. By integrating evolutionary, structural, and biochemical information, a surface segment, outside of its known receptor contacts, is predicted as a second interaction site that may help to further define its roles in energy balance and its functional differences between humans and other mammals.
Supporting ARINC 653-based Dynamic Reconfiguration (2012)

As the software for avionics becomes more complex, the challenge to provide the required reliability and safety mechanisms becomes also more complex. Embracing the ARINC 650 and 653 standards to provide a means to embark several systems into a single hardware cabinet opens the door to the development of even more elaborated software avionics systems, by overcoming the space constraints once found in this kind of system. Nevertheless, ARINC 653 exhibits also some limitations regarding fault redundancy management, especially when handling redundant applications with spares that back up other applications. In this paper, a framework to support fault tolerance and reconfiguration in avionics systems under the umbrella of ARINC 653 standard is described. This paper represents part of the results of the research projects carried out during the last two years by Eurocopter Espana in collaboration with the University of Castilla-La Mancha.
Indexing and Querying XML Data for Regular Path Expressions (2001)

With the advent of XML as a standard for data representation and exchange on the Internet, storing and querying XML data becomes more and more important. Several XML query languages have been proposed, and the common feature of the languages is the use of regular path expressions to query XML data. This poses a new challenge concerning indexing and searching XML data, because conventional approaches based on tree traversals may not meet the processing requirements under heavy access requests. In this paper, we propose a new system for indexing and storing XML data based on a numbering scheme for elements. This numbering scheme quickly determines the ancestor-descendant relationship between elements in the hierarchy of XML data. We also propose several algorithms for processing regular path expressions, namely, (1) ##-Join for searching paths from an element to another, (2) ##-Join for scanning sorted elements and attributes to find element-attribute pairs, and (3) ##-Join for finding Kleene-Closure on repeated paths or elements. The ##-Join algorithm is highly effective particularly for searching paths that are very long or whose lengths are unknown. Experimental results from our prototype system implementation show that the proposed algorithms can process XML queries with regular path expressions by up to an or- # This work was sponsored in part by National Science Foundation CAREER Award (IIS-9876037) and Research Infrastructure program EIA-0080123. The authors assume all responsibility for the contents of the paper. Permission to copy without fee all or part of this material is granted provided that the copies are not made or distributed for direct commercial advantage, the VLDB copyright notice and the title of the publication and its...
Un modello per la diffusione di co prodotto da traffico autoveicolare per la Città di Bari (1999)

In several towns the control of pollution produced by vehicular traffic represents a problematic task when models reported in litterature are commercially avalaible software are used. The difficulties are due both to the not availability of all meteorologic input needing, and, mainly, to lack of up-to-date and suitable traffic volumes and average speed in the main roads, essential parameters In this sort of models. On this basis it rised the nacessity of realizing simple models, such as ATDL (Atmospheric Turbolence and Diffusion Laboratory) by Gifford & Hanna, properly modified to work In short time scale.
II. Zur Lehre vom Tutor suspectus (1914)

absentia des Vormunds (D. h.t. 7 § §2.3; C. 5, 43, 3; D. h.t. 8
Antenna downtilt effects on CDMA cell-site capacity (2003)

Antenna downtilt is often used in cellularized mobile wireless communications systems to reduce inter-cell interference, particularly in a CDMA-based network where all call traffic in all cell sites is simultaneously supported using the same carrier frequency. While the inter-cell interference is indeed reduced through such downtilt the sectorization efficiency may also be reduced with increased downtilt. For a CDMA system, interference reduction increases cell-site capacity whereas reduced sectorization efficiency decreases cell-site capacity. Since the net effect on capacity is of interest, an analytical treatment of the effects of antenna downtilt on the reverse-link capacity for a CDMA cell site has been performed. Results from a parametric study for various cell-site configurations are presented in this paper.
Optically Flat Micromirror Using Stretched Membrane with Crystallization-Induced Stress (2007)

The flat and light-weighted micromirror is realized using the tense poly-Si film across a rigid c-Si drum. The tensile stress of ~600 MPa is obtained using the crystallization of a-Si film. Compared to the research carried out by Nee et al., the initial film has the purer amorphous phase and generates larger stress. The mirror satisfies the better optical flatness <lambda/10 for the visible light. The peak-to-valley distance of the micromirror is ~20 nm. The membrane profile is found to depend on the process sequence relating to the timing of the annealing. The mirror shape is stable against the high temperature.
Chromatic Changes in Polyaniline Films (1990)

The reflection intensities of polyaniline films (PANI) in the range of 250–800 nm have been determined using a reflection spectrometer at different potentials and pHs. The effect of potential and pH on the colors of PANI and the mechanism of the redox reactions have been discussed.
Inclusion of Multiple Fragment Types in the Site Identification by Ligand Competitive Saturation (SILCS) Approach (2013)

The site identification by ligand competitive saturation (SILCS) method identifies the location and approximate affinities of small molecular fragments on a target macromolecular surface by performing molecular dynamics (MD) simulations of the target in an aqueous solution of small molecules representative of different chemical functional groups. In this study, we introduce a set of small molecules to map potential interactions made by neutral hydrogen bond donors and acceptors and charged donor and acceptor fragments in addition to nonpolar fragments. The affinity pattern is obtained in the form of discretized probability or, equivalently, free energy maps, called FragMaps, which can be visualized with the target surface. We performed SILCS simulations for four proteins for which structural and thermodynamic data is available for multiple diverse ligands. Good overlap is shown between high affinity regions identified by the FragMaps and the crystallographic positions of ligand functional groups with similar chemical functionality, thus demonstrating the validity of the qualitative information obtained from the simulations. To test the ability of FragMaps in providing quantitative predictions, we calculate the previously introduced ligand grid free energy (LGFE) metric and observe its correspondence with experimentally measured binding affinity. LGFE is computed for different conformational ensembles and improvement in prediction is shown with increasing ligand conformational sampling. Ensemble generation includes a Monte Carlo sampling approach that uses the GFE FragMaps directly as the energy function. The results show that some but not all experimental trends are predicted and warrant improvements in the scoring methodology. In addition, the potential utility of atom-based free energy contributions to the LGFE scores and the use of multiple ligands in SILCS to identify displaceable water molecules during ligand design are discussed.
The Immunogenicity of New Insulins (1985)

Treatment with conventional insulin preparations results in appreciable insulin antibody formation in nearly all subjects. High titers of insulin antibodies are undesirable. They might induce insulin allergy and insulin resistance, and they influence metabolic regulation. Also, lipoatrophy is related to the immunogenicity of insulin preparations. Insulin antibodies are transferred from insulin-treated diabetic mothers to the fetus and contribute to the increase of free plasma insulin in the fetus, thus influencing the development of macrosomia and neonatal hypoglycemia. Therefore, the risk of insulin antibody formation should be reduced. This is possible by using purified, nonbeef insulin preparations. The risk of insulin allergy, insulin resistance, lipoatrophy, and prolonged hypoglycemia in mother and child is reduced by treating pregnant diabetic subjects with purified pork or human insulin preparations. The reduction of insulin requirement that accompanies the change from conventional insulin to purified nonbeef insulin treatment will compensate to some extent for the higher costs of new insulins.