gebhart/patrepeval huggingface dataset:
Systematic review: interactions between aspirin, and other nonsteroidal anti‐inflammatory drugs, and polymorphisms in relation to colorectal cancer (2014)

Knowledge of underlying biological effects of NSAIDs in relation to CRC is scarce and the basis for stratifying the patients for preventive treatment is not yet available. Further studies assessing interactions between long-term NSAID exposure and genetic variation in relation to CRC are warranted in large well-characterised prospective cohorts.
Expression of human terminal deoxynucleotidyl transferase in Escherichia coli. (1985)

A cloned DNA fragment related to pT17 containing a partial cDNA sequence of human terminal deoxynucleotidyl transferase was used as a probe to screen for the full length cDNA sequence of the enzyme in a lambda gt11 library constructed from human lymphoblastoid KM-3 cDNA. A recombinant containing a 2068-base pair insert was isolated and recloned into the EcoRI site of the sequencing plasmic pUC-8 as two subclones, pT711 and pT106. DNA sequencing and hybridization studies showed that pT711 contains the pT17 sequence and an additional 172 upstream nucleotides. pT711 represents the coding sequence for the carboxyl half of the terminal transferase protein. pT106, containing a 965-base pair insert, hybridizes to the same mRNA as pT711 on Northern blots and contains an open reading frame that is in phase with the reading frame of the insert in pT711. Amino acid sequencing of the 58-kDa peptide of the calf thymus terminal transferase failed, indicating that the N terminus is blocked. N-Terminal sequencing of a 56-kDa form of the protein produced 24 amino acids corresponding to the translated human cDNA coding sequence starting at residue 398 of the insert in pT106 with 83% homology between bovine and human sequence. The initiation codon is assigned to an ATG sequence at nucleotide 329 of the insert in pT106. Comparison of the translated human terminal transferase sequence with peptides from the calf thymus enzyme showed that the homology between the human and bovine enzyme is better than 90% among 263 amino acids determined. The coding sequences in pT106 and pT711 were recloned into an expression plasmid pUC-19 downstream from the lac promoter and in phase with the coding sequence of the lac Z gene. Lysates of bacteria carrying the reconstructed coding sequence of human terminal transferase contain a fused protein of 60 kDa that reacts with rabbit antibody to terminal transferase on immunoblots and exhibits enzyme activity. Isolation of this fused protein from bacterial lysates with mouse monoclonal antibody to human terminal transferase produces the expected protein of 60 kDa.
The Level of Intracellular Glutathione Is a Key Regulator for the Induction of Stress-Activated Signal Transduction Pathways Including Jun N-Terminal Protein Kinases and p38 Kinase by Alkylating Agents (1997)

Monofunctional alkylating agents like methyl methanesulfonate (MMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) are potent inducers of cellular stress leading to chromosomal aberrations, point mutations, and cell killing. We show that these agents induce a specific cellular stress response program which includes the activation of Jun N-terminal kinases/stress-activated protein kinases (JNK/SAPKs), p38 mitogen-activated protein kinase, and the upstream kinase SEK1/MKK4 and which depends on the reaction mechanism of the alkylating agent in question. Similar to another inducer of cellular stress, UV irradiation, damage of nuclear DNA by alkylation is not involved in the MMS-induced response. However, in contrast to UV and other inducers of the JNK/SAPKs and p38 pathways, activation of growth factor and G-protein-coupled receptors does not play a role in the MMS response. We identified the intracellular glutathione (GSH) level as critical for JNK/SAPK activation by MMS: enhancing the GSH level by pretreatment of the cells with GSH or N-acetylcysteine inhibits, whereas depletion of the cellular GSH pool causes hyperinduction of JNK/SAPK activity by MMS. In light of the JNK/SAPK-dependent induction of c-jun and c-fos transcription, and the Jun/Fos-induced transcription of xenobiotic-metabolizing enzymes, these data provide a potential critical role of JNK/SAPK and p38 in the induction of a cellular defense program against cytotoxic xenobiotics such as MMS.
Focused ion beam milling of vitreous water: prospects for an alternative to cryo‐ultramicrotomy of frozen‐hydrated biological samples (2006)

The feasibility of using a focused ion beam (FIB) for the purpose of thinning vitreously frozen biological specimens for transmission electron microscopy (TEM) was explored. A concern was whether heat transfer beyond the direct ion interaction layer might devitrify the ice. To test this possibility, we milled vitreously frozen water on a standard TEM grid with a 30-keV Ga(+) beam, and cryo-transferred the grid to a TEM for examination. Following FIB milling of the vitreous ice from a thickness of approximately 1200 nm to 200-150 nm, changes characteristic of heat-induced devitrification were not observed by TEM, in either images or diffraction patterns. Although numerous technical challenges remain, it is anticipated that 'cryo-FIB thinning' of bulk frozen-hydratred material will be capable of producing specimens for TEM cryo-tomography with much greater efficiency than cryo-ultramicrotomy, and without the specimen distortions and handling difficulties of the latter.
Technical overview of IBM's Java initiatives (1998)

This paper gives an overview of some of IBM's major Java™ efforts and sets forth a structure that relates the individual Java efforts to one another. The paper describes IBM's overall approach toward Java and describes how IBM is exploiting Java to answer customer requirements in such areas as server platforms, reusable components, and tools. This paper will serve as an introduction to some of the other papers that are included in this issue of the IBM Systems Journal and that detail individual areas of IBM's focus on Java.
Search Engine (2011)

This chapter presents technologies and approaches for information retrieval in a knowledge base. We intend to show that the use of ontology for domain representation and knowledge search offers a more efficient approach for knowledge management. This approach focuses on the meaning of the word, thus becoming an important element in the building of the Semantic Web. The search based on both keywords and ontology allows more effective information retrieval exploiting the Semantic of the information in a variety of data. We present a method for taxonomy building, annotating, and searching documents with taxonomy concepts. We also describe our experience in the creation of an informal taxonomy, the automatic classification, and the validation of search results with traditional measures, such as precision, recall and f-measure.
627. Organosilicon compounds. Part VI. The kinetics of the acid-catalysed cleavage of p-methoxyphenyltrimethylsilane (1953)

C. Eaborn, J. Chem. Soc., 1953, 3148 DOI: 10.1039/JR9530003148
An insight of traditional plasmid curing in Vibrio species (2015)

As the causative agent of foodborne related illness, Vibrio species causes a huge impact on the public health and management. Vibrio species is often associated with seafood as the latter plays a role as a vehicle to transmit bacterial infections. Hence, antibiotics are used not to promote growth but rather to prevent and treat bacterial infections. The extensive use of antibiotics in the aquaculture industry and environment has led to the emerging of antibiotic resistant strains. This phenomenon has triggered an alarming public health concern due to the increase number of pathogenic Vibrio strains that are resistant to clinically used antibiotics and is found in the environment. Antibiotic resistance and the genes location in the strains can be detected through plasmid curing assay. The results derived from plasmid curing assay is fast, cost effective, sufficient in providing insights, and influence the antibiotic management policies in the aquaculture industry. This presentation aims in discussing and providing insights on various curing agents in Vibrio species. To our best of knowledge, this is a first review written discussing on plasmid curing in Vibrio species.
Low-latency asynchronous FIFO's using token rings (2002)

This paper presents several new asynchronous FIFO designs. While most existing FIFO's trade higher throughput for higher latency, our goal is to achieve very low latency while maintaining good throughput. The designs are implemented as circular arrays of cells connected to common data buses. Data items are not moved around the array once they are enqueued. Each cell's input and output behavior is dictated by the flow of two tokens around the ring: one that allows enqueuing data and one that allows dequeuing data. Two novel protocols are introduced with various degrees of parallelism, as well as four different implementations. The best simulation results, in 0.6 /spl mu/, have a latency of 1.73 ns and throughput of 454 MegaOperations/second for a 4-place FIFO.
Panning of Antibody Phage-Display Libraries: Standard Protocols (2003)

Recombinant antibody (Ab) libraries have been constructed from a wide range of B-lymphocyte sources using a number of different approaches. Sizes of the libraries that have been produced vary considerably, from small libraries of 106 up to large libraries>1010. Often an Ab with the desired specificity exists at low frequencies in the recombinant Ab repertoire. It is therefore necessary to have an effective technique for the enrichment and identification of a desired Ab from a heterogenous repertoire.
Arabia and the gulf: from traditional society to modern states. Essays in honour of M. A. Shaban's 60th birthday (16th November 1986). Edited by Ian Richard Netton. pp. xviii, 259, 1 map. London and Sydney, Croom Helm, 1986. £ 29.95. (1988)

Arabia and the gulf: from traditional society to modern states. Essays in honour of M. A. Shaban's 60th birthday (16th November 1986). Edited by Ian Richard Netton. pp. xviii, 259, 1 map. London and Sydney, Croom Helm, 1986. £ 29.95. - Volume 120 Issue 1
Viscosity and Diffusion Constants Calculation of n-Alkanes by Molecular Dynamics Simulations (2003)

In this paper we have presented the results for viscosity and self-diffusion constants of model systems for four liquid n-alkanes (C-12, C-20, C-32, and C-44) in a canonical ensemble at several temperatures using molecular dynamics (MD) simulations. The small chains of these n-alkanes are clearly <Ree(2)>/6 <R-g(2)>>(1), which leads to the conclusion that the liquid n-alkanes over the whole temperatures considered are far away from the Rouse regime. Calculated viscosity eta and self-diffusion constants D are comparable with experimental results and the temperature dependence of both 17 and D is suitably described by the Arrhenius plot. The behavior of both activation energies, E-eta and E-D, with increasing chain length indicates that the activation energies approach asymptotic values-as n increases to the higher value, which is experimentally observed. Two calculated monomeric friction constants zeta and zeta(D) give a correct qualitative trend: decrease with increasing temperature and increase with increasing chain length n. Comparison of the time auto-correlation functions of the end-to-end vector calculated from the Rouse model for n-dodecane (C-12) at 273 K and for n-tetratetracontane (C-44) at 473 K with those extracted directly from our MD simulations confirms that the short chain n-alkanes considered in this study are far away from the Rouse regime.
Class E tuned power amplifier with nonsinusoidal output voltage (1986)

An analysis is presented of the amplifier for a limiting case when the load network does not contain a series-resonant output circuit and the output voltage is non-sinusoidal. For optimum operation with any switch-duty ratio, the author has determined the current and voltage waveforms, the collector current and collector-emitter peak values, the output power, the power-output capability, and the load-network component values. The spectrum of the output voltage is given for a switch-duty ratio of 0.25, 0.5, and 0.75. Close-approximation equations are given for transistor power losses and collector efficiency. The experimental and theoretical results are in very good agreement. The measured collector efficiency is 95%. The circuit has practical applications, e.g., in high-efficiency switching-mode DC-to-DC converters used in DC power supplies for microcomputers or communication equipment.
VASCULAR REMODELING IN EXPERIMENTALLY INDUCED SUBACUTE CANINE PULMONARY HYPERTENSION (2001)

We quantified in vivo pulmonary vascular remodeling in a large animal model of pulmonary hypertension (PH). In group PH (n = 6), 3 mg/kg dehydromonocrotaline (DHMC) was administered to 12-week-old beagles via a right atrial injection. Eight weeks after DHMC in group PH, pulmonary artery pressure increased significantly (P < .05) from 18 +/- 2 mm Hg at baseline to 30 +/- 4 mm Hg. Medial wall thickness and medial wall area as a percentage of total vessel diameter or area was significantly higher (P < .05) in group PH (29 +/- 9% and 48 +/- 12%) than in a control group (n = 5) (7 +/- 1% and 14 +/- 1%). Neointimal proliferation was observed in 42% of pulmonary arterioles in the PH group but never in the control group. We conclude that a single injection of DHMC in young beagles, in addition to the development of moderate degrees of PH after 8 weeks, causes significant pulmonary vascular remodeling, with features similar to those observed in patients with primary PH.
Polymerization of Lyotropic Liquid Crystals (1989)

The polymerization of one or more components of a lyotropic liquid crystal in such a way as to preserve and fixate the microstructure has recently been successfully performed. This opens up new avenues for the study and technological application of these periodic microstructures. Of particular importance are the so-called bicontinuous cubic phases, having triply-periodic microstructures in which aqueous and hydrocarbon components are simultaneously continuous. It is shown that the polymerization of one of these components, followed by removal of the liquid components, leads to the first microporous polymeric material exhibiting a continuous, triply-periodic porespace with monodisperse, nanometer-sized pores. It is also shown that proteins can be immobilized inside of polymerized cubic phases to create a reaction medium allowing continuous flow of reactants and products, and providing a natural lipid environment for the proteins.
Oligoclonal Antibody Targeting Ghrelin Increases Energy Expenditure and Reduces Food Intake in Fasted Mice (2011)

Ghrelin, an enteric peptide hormone linked to the pathophysiology of obesity has been a therapeutic target of great interest over the past decade. Many research efforts have focused on the antagonism of ghrelin's endogenous receptor GHSR1a, which is found along ascending vagal afferent fibers, as well as in the arcuate nucleus of the hypothalamus. Additionally, peptidic inhibitors of ghrelin O-acyltransferase, the enzyme responsible for the paracrine activation of ghrelin, have recently been studied. Our research has taken an alternative immunological approach, studying both active and passive vaccination as a means to sequester ghrelin in the periphery, with the original discovery in rat of decreased feed efficiency and adiposity, as well as increased metabolic activity. Using our previous hapten designs as a stepping-stone, three monoclonal antibodies (JG2, JG3, and JG4) were procured against ghrelin and tested in vivo. While mAb JG4 had the highest affinity for ghrelin, it failed to attenuate the orexigenic effects of food deprivation on energy metabolism or food intake in mice. However, animals that were administered a combination of JG3:JG4 (termed a doublet) or JG2:JG3:JG4 (termed a triplet) demonstrated higher heat dispersion and rate of respiration (higher CO(2) emission and O(2) consumption) during a 24 h fast refeed. Mice administered the triplet cocktail of JG2:JG3:JG4 also demonstrated decreased food intake upon refeeding as compared to control animals. Recently, Lu and colleagues reported that a passive approach using a single, high affinity N-terminally directed monoclonal antibody did not abrogate the effects of endogenous ghrelin. Our current report corroborates this finding, yet, refutes that a monoclonal antibody approach cannot be efficacious. Rather, we find that a multiple monoclonal antibody (oligoclonal) approach can reproduce the underlying logic to previously reported efficacies using active vaccinations.
Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg (1990)

Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in the genome-linked protein VPg-coding region. The transfecting RNA was transcribed in vitro from full-length poliovirus type 1 (Mahoney) cDNA containing a VPg mutagenesis cartridge. Hybridization experiments using ribonucleotide probes specific for the 3' end of positive- and negative-sense poliovirus RNA indicated that all mutant RNAs encoding a linking tyrosine in position 3 or 4 of VPg were replicated even though no virus was produced. VPg, but no VPg precursor, was found to be linked to the 5' end of the newly synthesized RNA. Encapsidated mutant RNAs were not found in transfected-cell lysates. After extended maintenance of transfected HeLa cells, a viable revertant of one of the nonviable RNAs was recovered; the revertant lost the lethal lesion in VPg by restoring the wild-type amino acid, but it retained all other nucleotide changes introduced during construction of the mutagenesis cartridge. Mutant RNA encoding phenylalanine or serine rather than tyrosine, the linking amino acid in VPg, was not replicated in transfected cells. A chimeric mutant containing the VPg-coding region of coxsackievirus within the poliovirus genome was viable but displayed impaired multiplication. A poliovirus-coxsackievirus chimera lacking a linking tyrosine in VPg was nonviable and replication-negative. The results indicate that a linkage-competent VPg is necessary for poliovirus RNA synthesis to occur but that a step in poliovirus replication other than initiation of RNA synthesis can be interrupted by lethal mutations in VPg.
New polynuclear carbonyl complexes containing iron with cobalt or rhodium (1970)

The reaction of Fe2(CO)9 with (π-C5H5)M(CO)2](M = Co or Rh) has led to the preparation of five new polynuclear carbonyl complexes containing iron with cobalt or rhodium. The structure of these new ‘mixed-metal’ complexes has been inferred from a combination of i.r., n.m.r., and Mössbauer spectroscopic data and is discussed in relation to the structures of known polynuclear carbonyls such as Fe3(CO)12 and (π-C5H5)3Rh3(CO)3.
Long-period gratings in wavelength-scale microfibers (2009)

We report the fabrication of long-period gratings (LPGs) in wavelength-scale microfibers with diameters from 1.5 to 3 microm. The LPGs were fabricated by use of a femtosecond IR laser to periodically modify the surface of the fibers. These LPGs have grating periods of a few tens of micrometers, much smaller than those in conventional optical fibers. A compact 10-period LPG with a device length of only approximately 150 microm demonstrated a strong resonant dip of >20 dB around 1330 nm. These microfiber LPGs would be useful in-fiber components for microfiber-based devices, circuits, and sensors.
Neutrophil labeling with indium-111: tropolone vs. oxine. (1983)

This study was undertaken to compare tropolone with oxine (8-hydroxy-quinoline) for labeling human neutrophils with In-111. Exposure of neutrophils to tropolone at concentrations required for efficient labeling resulted in a marked impairment of chemotaxis. In contrast, no impairment of neutrophil chemotaxis was observed using In-111 oxine. Labeling efficiencies obtained with In-111 tropolone under optimal conditions were consistently less than those obtained with In-111 oxine. We evaluated cells labeled by the two methods using chemotaxis radioassay to assess the chemotatic potential of labeled cells. The results led to the conclusion that the oxine technique is preferable to tropolone for labeling human neutrophils with In-111.
[Texto 3] (1999)

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In Situ Infrared Spectroelectrochemistry (1985)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIn Situ Infrared SpectroelectrochemistryJohn Κ. Foley and Stanley PonsCite this: Anal. Chem. 1985, 57, 8, 945APublication Date (Print):July 1, 1985Publication History Published online29 May 2012Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/ac00285a833https://doi.org/10.1021/ac00285a833research-articleACS PublicationsRequest reuse permissionsArticle Views232Altmetric-Citations21LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Heart Rate Measurement Using Video in Different User States for Online HCI Applications (2014)

Abstract In this paper we implemented an unobtrusive and non-invasive method to measure pulse rate and heart rate variability. A Ballistocardiography technique has been used which describes ballistic force applied by heart on blood vessels. Ballistocardiography depicts repetitive motion in human body against blood flow because of the ballistic force. In this process videos of human head have been recorded and feature points using Lucas - Kanade point tracker are calculated. After filtering the resultant signal to remove noise (unwanted signals) trajectories are decomposed into elementary components. Component that best corresponds to pulse frequency is selected. Detected peaks correspond to beats in the signal and hence the Heart Rate Variability is calculated. The algorithm was tested with many videos of 12 subjects (10 males and 2 females) with variations in skin color, sex, subject's state. We found that user state and activities affect Heart Rate Variability which can be calculated through our algorithm. It is concluded that extensive research and development of this unobtrusive and non-invasive technique can yield new tools for various online and adaptive HCI applications.