gebhart/patrepeval huggingface dataset:
Correlates and outcomes related to periprocedural myocardial injury during percutaneous coronary intervention for chronic total occlusion: Results from a prospective, single center PCI registry (2016)

In this unique Chinese cohort, PMI post CTO PCI was associated with several clinical and angiographic factors and exerts an adverse effect on 1-year clinical outcomes.
Generation of lymphokine-activated killer cell activity from human thymocytes. (1987)

We have generated lymphokine-activated killer (LAK) cells from human thymocytes in order to assess the relationship between LAK cells and T cells. Fresh thymocytes lack natural cytotoxic activity, and cytotoxicity cannot be stimulated by short term (1 hr) incubation with interferon or recombinant interleukin 2 (rIL-2). In addition, thymocytes are phenotypically devoid of cells bearing the natural killer (NK)-associated markers cluster designation (CD) 16 and NKH-1. After culture for 5 to 8 days with rIL-2, thymocytes display high levels of cytotoxic activity against both NK-sensitive and NK-resistant targets. Thymocytes require slightly more IL-2 than do peripheral blood lymphocytes to generate LAK activity. We have examined the phenotype of the thymocyte LAK precursor and effector cells. Thymocyte LAK precursors are of low to medium density, CD1-negative, and predominantly CD3-negative. Although CD3-positive cells proliferate in response to rIL-2, they are low in cytolytic capabilities. The effector cells, like the LAK precursors, are low to medium density lymphocytes. The cytotoxic cells are predominantly CD3-negative, and cytotoxic activity cannot be blocked with the use of anti-CD3 monoclonal antibodies. The effector cells also lack most NK-associated markers (HNK-1, and the CD16 markers Leu-11b and B73.1) but possess the NK-associated marker NKH-1 (N901). The responsive cell appears to be at a very early stage of thymic development, and it does not appear to either require or express the CD3-T cell receptor complex.
Aggregation-Mediated Macromolecular Uptake by a Molecular Transporter (2013)

Endocytosis is a key process in cellular delivery of macromolecules by molecular transporters, although the mechanism of internalization remains unclear. Here, we probe the cellular uptake of streptavidin using biotinylated guanidinoneomycin (biotinGNeo), a low molecular weight guanidinium-rich molecular transporter. Two distinct modes were explored: (i) incubation of cells with a preformed tetravalent streptavidin-(biotinGNeo)4 conjugate and (ii) preincubation of cells with the biotinGNeo before exposure to streptavidin. A significant enhancement in uptake was observed after preincubation with biotinGNeo. FRET studies showed that the enhanced uptake was accompanied by extensive aggregation of streptavidin on the cell surface. Because guanidinylated neomycin was previously found to exclusively bind to heparan sulfate, our observations suggest that heparan sulfate proteoglycan aggregation is a pivotal step for endocytic entry into cells by guanidinoglycosides. These observations put forward a practical and general pathway for the cellular delivery of diverse macromolecules.
Asymmetric Synthesis of .alpha.-Aminophosphonates via Diastereoselective Addition of Lithium Diethyl Phosphite to Chelating Imines (1994)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAsymmetric Synthesis of .alpha.-Aminophosphonates via Diastereoselective Addition of Lithium Diethyl Phosphite to Chelating IminesKraig M. Yager, Carol M. Taylor, and Amos B. Smith IIICite this: J. Am. Chem. Soc. 1994, 116, 20, 9377–9378Publication Date (Print):October 1, 1994Publication History Published online1 May 2002Published inissue 1 October 1994https://doi.org/10.1021/ja00099a084RIGHTS & PERMISSIONSArticle Views673Altmetric-Citations88LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (277 KB) Get e-AlertsSupporting Info (2)»Supporting Information Supporting Information Get e-Alerts
Spontaneous four-wave mixing in microring resonators (2010)

We consider spontaneous four-wave mixing in a microring resonator, presenting photon-pair generation rates and biphoton wave functions. We show how generation rates can be simply predicted from the performance of the device in the classical regime and that a wide variety of biphoton wave functions can be achieved by varying the pump pulse duration.
Thioether Complexes of Palladium(II) and Platinum(II) as Artificial Peptidases. Residue-Selective Peptide Cleavage by a Palladium(II) Complex (2005)

We report the synthesis and characterization of perchlorate salts containing the following three novel complex cations each with a bidentate thioether ligand: binuclear cis-[Pt(CH3SCH2CH2CH2SCH3)(mu-OH)]22+, mononuclear cis-[Pt(CH3SCH2CH2CH2SCH3)(H2O)2]2+, and mononuclear cis-[Pd(CH3SCH2CH2CH2SCH3)(H2O)2]2+. Despite their analogous compositions, the mononuclear Pt(II) and Pd(II) complexes differ in the selectivity with which they promote the hydrolysis of polypeptides. The complex cis-[Pt(CH3SCH2CH2CH2SCH3)(H2O)2]2+ promotes slow but selective cleavage of Met-Pro peptide bonds at pH 2.0. The selectivity of the complex cis-[Pd(CH3SCH2CH2CH2SCH3)(H2O)2]2+ is pH-dependent. At pH 2.0, this Pd(II) complex promotes residue-selective hydrolysis of the X-Y bond in X-Y-Met and X-Y-His sequences; the rate is enhanced when residue Y is proline. At pH 7.0, this kinetic preference becomes sequence-selective in that the Pd(II) complex exclusively cleaves the X-Pro bond in X-Pro-Met and X-Pro-His sequences. The enhanced reactivity of the X-Pro amide group is attributed to the high basicity of its carbonyl oxygen atom. Binding of the metal(II) atom enhances the electrophilicity of the carbonyl carbon atom and promotes nucleophilic attack by a solvent water molecule. The bidentate thioether ligand disfavors the formation of hydrolytically unreactive complexes, allowing the Pd(II) complex to promote the cleavage reaction.
Model-driven development of large-scale Web applications (2004)

This paper describes our approach to support the development of large-scale Web applications. Large development efforts have to be divided into a number of smaller tasks of different kinds that can be performed by multiple developers. Once this process has taken place, it is important to manage the consistency among the artifacts in an efficient and systematic manner. Our model-driven approach makes this possible. In this paper, we discuss how a metamodel is used to describe part of the specification as a central contract among the developers. We also describe a tool that we implemented on the basis of the metamodel. The tool provides a variety of code generators and a mechanism for checking whether view artifacts, such as JavaServer Pages™, are compliant with the model. This feature helps developers manage the consistency between a view artifact and the related business logic—HyperText Transfer Protocol request handlers.
In vitro metabolism of indinavir in the human fetal liver microsomes. (1997)

In vitro microsomal formation of primary metabolites of indinavir (CRIXIVAN, MK-0639, L-735,524), an HIV protease inhibitor, were qualitatively similar among the different developmental stages in humans, although the fetal liver had a lower capability to form the metabolites than the pediatric and adult liver. The lower activity of fetal liver was mainly owing to a decrease in the Vmax values. The Vmax value in the fetus was about one-third of that in the adult human, while no significant difference was found in Km values between groups. The liver microsomes were also characterized using P450 markers to examine the development-associated alteration in P450 functional activities. Debrisoquine 4-hydroxylase activity was comparable among the three age groups. In contrast, tolbutamide methyl hydroxylase activity, as well as the CYP3A marker, testosterone 6beta-hydroxylase activity, in the fetal liver microsomes was much lower than in the pediatric and adult by more than 40-fold. However, the difference in testosterone 2beta-hydroxylase and nifedipine N-oxidase activities between fetus and adult was markedly smaller. The ratio of indinavir metabolism in pediatric or adult liver to fetus was 1.7 for pediatric and 3.6 for adult liver microsomes. Similarly, testosterone 2beta-hydroxylase and nifedipine N-oxidase activities showed smaller differences between adult (or pediatric) and fetal liver microsomes than testosterone 6beta-hydroxylase activity. The reason for the observed marked differences in the development-associated alteration may lie in the differences of substrate specificities between CYP3A isoforms.
Effect of Endotoxin on Granulopoiesis and the In Vitro Colony-forming Cell (1973)

Abstract The effect of endotoxin on the tibial in vitro colony-forming cell (CFC) and the differentiated granulocyte compartment was evaluated. The injection of 5 µg of Salmonella typhosa endotoxin into CF1 mice leads to elevated serum colony-stimulating factor (CSF) levels along with a transient granulocytopenia followed by the release of granulocytes from the marrow. The present study demonstrates that the number of tibial CFC, as assayed on soft agar, decreases within 20 min after endotoxin, reaches a nadir at 6 hr (65.8 ± 4.9% of control), and then returns to control values by 48 hr. Concomitantly, over the 48 hr after endotoxin, there are sequential increases in the marrow myeloblast-promyelocyte compartment (MPC), myelocyte compartment (MC), and the PMN compartment. Radioautographic studies utilizing 3HTdR showed increased labeling indices and grain counts in the MPC and MC at 24 or 48 hr after endotoxin, indicating a shortening of generation time and perhaps of the DNA synthetic period in these compartments. The present data suggest that elevated CSF levels seen after endotoxin administration may lead to differentiation of the marrow CFC into the granulocyte pathway. There are also changes in the differentiated granulocyte compartment, suggesting a decreased generation time that may be due to CSF or a separate regulator such as antichalone.
Active Components of Ginger Potentiate β-Agonist–Induced Relaxation of Airway Smooth Muscle by Modulating Cytoskeletal Regulatory Proteins (2013)

β-Agonists are the first-line therapy to alleviate asthma symptoms by acutely relaxing the airway. Purified components of ginger relax airway smooth muscle (ASM), but the mechanisms are unclear. By elucidating these mechanisms, we can explore the use of phytotherapeutics in combination with traditional asthma therapies. The objectives of this study were to: (1) determine if 6-gingerol, 8-gingerol, or 6-shogaol potentiate β-agonist-induced ASM relaxation; and (2) define the mechanism(s) of action responsible for this potentiation. Human ASM was contracted in organ baths. Tissues were relaxed dose dependently with β-agonist, isoproterenol, in the presence of vehicle, 6-gingerol, 8-gingerol, or 6-shogaol (100 μM). Primary human ASM cells were used for cellular experiments. Purified phosphodiesterase (PDE) 4D or phospholipase C β enzyme was used to assess inhibitory activity of ginger components using fluorescent assays. A G-LISA assay was used to determine the effects of ginger constituents on Ras homolog gene family member A activation. Significant potentiation of isoproterenol-induced relaxation was observed with each of the ginger constituents. 6-Shogaol showed the largest shift in isoproterenol half-maximal effective concentration. 6-Gingerol, 8-gingerol, or 6-shogaol significantly inhibited PDE4D, whereas 8-gingerol and 6-shogaol also inhibited phospholipase C β activity. 6-Shogaol alone inhibited Ras homolog gene family member A activation. In human ASM cells, these constituents decreased phosphorylation of 17-kD protein kinase C-potentiated inhibitory protein of type 1 protein phosphatase and 8-gingerol decreased myosin light chain phosphorylation. Isolated components of ginger potentiate β-agonist-induced relaxation in human ASM. This potentiation involves PDE4D inhibition and cytoskeletal regulatory proteins. Together with β-agonists, 6-gingerol, 8-gingerol, or 6-shogaol may augment existing asthma therapy, resulting in relief of symptoms through complementary intracellular pathways.
Measuring intrinsic quality of semantic search based on feature vectors (2010)

Search is probably the most frequent activity on the web. Yet, it is not effortless, mainly due to heterogeneous information resources. Semantic search is a means to tackle the problem of ambiguity. In this paper, we analyse a process of constructing semantic-linguistic Feature Vectors (FVs) used in our semantic search approach. These FVs are built based on domain semantics encoded in an ontology and enhanced by relevant terminology from web documents. Since FVs are central building blocks of the approach, we investigate the quality of FVs. We take a closer look at the process of FV construction and the impact of chosen techniques on the quality of FVs. We report on a set of laboratory experiments and analyse aspects affecting the FV quality and the FV construction error rates.
Run-Time Reconfiguration of Tandem Inverter for Induction Motor Drives (2006)

The paper presents a short introduction to reconfigurable systems and why are they used or should be used in control of AC drives. The reconfigurable vector control system is introduced and there are treated the reconfiguration problems. There is analyzed the reconfigurable control system and it is motivated the need for reconfiguration in motor control. The control of the induction motor fed by the tandem inverter needs reconfiguration if the supply is made only from one inverter instead of the both component ones. Finally a simulation and fast prototyping method is shown using Field Programmable Gate Arrays.
Cell-Cell Transmission Enables HIV-1 to Evade Inhibition by Potent CD4bs Directed Antibodies (2012)

HIV is known to spread efficiently both in a cell-free state and from cell to cell, however the relative importance of the cell-cell transmission mode in natural infection has not yet been resolved. Likewise to what extent cell-cell transmission is vulnerable to inhibition by neutralizing antibodies and entry inhibitors remains to be determined. Here we report on neutralizing antibody activity during cell-cell transmission using specifically tailored experimental strategies which enable unambiguous discrimination between the two transmission routes. We demonstrate that the activity of neutralizing monoclonal antibodies (mAbs) and entry inhibitors during cell-cell transmission varies depending on their mode of action. While gp41 directed agents remain active, CD4 binding site (CD4bs) directed inhibitors, including the potent neutralizing mAb VRC01, dramatically lose potency during cell-cell transmission. This implies that CD4bs mAbs act preferentially through blocking free virus transmission, while still allowing HIV to spread through cell-cell contacts. Thus providing a plausible explanation for how HIV maintains infectivity and rapidly escapes potent and broadly active CD4bs directed antibody responses in vivo.
Disposition of the psychotropic drugs buspirone, MJ-13805 and piribedil, and of their common active metabolite 1-(2-pyrimidinyl)-piperazine in the rat (1985)

1-(2-Pyrimidinyl)-piperazine (PmP) is a common metabolite of the structurally related drugs buspirone, MJ-13805 and piribedil. After i.v. injection (25 mumol/kg) to rats, all three parent drugs are rapidly cleared with a t 1/2 (beta) of about 30 min. The metabolite t 1/2 is about four times that of its parent drugs. About 25, 23 and 2% of buspirone, MJ-13805 and piribedil, respectively, reaches the systemic circulation as PmP. The metabolite concentrates in the brain where its A.U.C. is about twice those of buspirone and MJ-13805. Results indicate that PmP formation is a pharmacologically significant process for both buspirone and MJ-13805 but it is probably less important for piribedil.
Advances in the theory and application of BSF cells (1975)

The characteristics and behavior of p(+), p solar cells were investigated. The p(+), p cells were made by the removal of the n(+) surface layers from n(+), p p(+), BSF cells followed by application of a suitable contact to the resultant p(+), p structures. The open circuit voltage of p(+), p cells was found to increase with increasing 'p' bulk resistivity. The measured open circuit velocity-temperature coefficients were positive and increased with increasing resistivity. An outline of prior limitations in solar cell design is presented, and the removal of these limitations through use of BSF effects is pointed out. The study of BSF effects made feasible production of very thin high efficiency silicon cells as well as high resistivity-high efficiency cells, two desirable types of silicon cells which were previously impossible to make.
Accurate Compensation of Attitude Angle Error in a Dual-Axis Rotation Inertial Navigation System (2017)

In the dual-axis rotation inertial navigation system (INS), besides the gyro error, accelerometer error, rolling misalignment angle error, and the gimbal angle error, the shaft swing angle and the axis non-orthogonal angle also affect the attitude accuracy. Through the analysis of the structure, we can see that the shaft swing angle and axis non-orthogonal angle will produce coning errors which cause the fluctuation of the attitude. According to the analysis of the rotation vector, it can be seen that the coning error will generate additional drift velocity along the rotating shaft, which can reduce the navigation precision of the system. In this paper, based on the establishment of the modulation average frame, the vector projection is carried out, and then the attitude conversion matrix and the attitude error matrix mainly including the shaft swing angle and axis non-orthogonal are obtained. Because the attitude angles are given under the static condition, the shaft swing angle and the axis non-orthogonal angle are estimated by the static Kalman filter (KF). This kind of KF method has been widely recognized as the standard optimal estimation tool for estimating the parameters such as coning angles (α₁ , α₂), initial phase angles (ϕ₁,ϕ₂), and the non-perpendicular angle (η). In order to carry out the system level verification, a dual axis rotation INS is designed. Through simulation and experiments, the results show that the amplitudes of the attitude angles' variation are reduced by about 20%-30% when the shaft rotates. The attitude error equation is reasonably simplified and the calibration method is accurate enough. The attitude accuracy is further improved.
Carbon-to-Nitrogen Ratio, Carbon Concentration, and Amino Acid Composition of Growth Media Influence Conidiation of Helminthosporium solani (1998)

Silver scurf, caused by Helminthosporium solani, is an important storage disease of potatoes. Experiments designed to evaluate control alternatives are limited by difficulty in producing conidial inoculum. In an effort to better understand how this difficulty could be overcome this study evaluated the influence of various carbon-to-nitrogen (C:N) ratios, carbon concentrations, and amino acids on conidial germination, colony diameter, and conidiation of Helminthosporium solani grown on solid-phase basal salts media. Under the conditions tested, the highest concentrations of conidia were produced with 1.25 to 2.5 g of carbon/L at a C:N ratio of 10:1. Higher C:N ratios or higher carbon concentrations reduced conidiation. Total conidia production was improved by use of tyrosine or arginine as the sole nitrogen source. Use of leucine, lysine, methionine, phenylalanine, or threonine severely inhibited H. solani conidia production. Use of a nitrogen source containing a mixture of amino acids resulted in a defined medium that permitted conidiation and growth of H. solani that was similar to or better than that obtained with standard V8 Juice medium.
PITUITARY‐OVARIAN FUNCTION BEFORE, DURING AND AFTER THE MENOPAUSE: A LONGITUDINAL STUDY (1982)

The excretion of FSH, LH, oestrogens and pregnanediol was monitored once weekly in urine samples collected from eight peri-menopausal women aged 44-55 years (median, 52 years). Observations were commenced between 5 and 15 weeks before the menopause and were continued for 22-30 weeks after final menstruation. Amenorrhoea of greater than 2 years duration in association with a persistent elevation in gonadotrophin output was considered proof of the post-menopausal state. No clear hormonal change occurred at the time of the menopause. During the peri-menopause there is a transition from the regular ovulatory cycles of pre-menopausal women to the unvarying high gonadotrophin and low oestrogen excretion which is generally regarded as being characteristic of post-menopausal women. In the group studied, post-menopausal levels of FSH and LH were common before and episodes of high oestrogen excretion were not uncommon after final menstruation. Menstrual failure appeared to occur spontaneously at some stage during the transition from the pre- to the post-menopausal state, and not to be associated with its conclusion. From the hormonal point of view the immediately post-menopausal period could not be distinguished from the long cycles of peri-menopausal women. This suggests that an endometrial rather than a hormonal event might determine the time at which menstruation stops during the menopausal transition.
"Close"and"open"in traditional Chinese gardens (2004)

Chinese attitude and thinking about the dialectical uniform relationship between human beings would conquer nature and people are a part of nature made the traditional Chinese gardens the shapetaking base.By analyzing the thought,overall plan,space form,architecture,et al.the contrast and connection between close and open in traditional Chinese gardens was expounded as well as the effects which had been produced in society and in esthetics .