gebhart/patrepeval huggingface dataset:
Molecular cloning and expression of the Escherichia coli dimethyl sulfoxide reductase operon (1988)

The dimethyl sulfoxide (DMSO) reductase operon coding for a membrane-bound iron-sulfur, molybdoenzyme, which functions as a terminal reductase in Escherichia coli, has been isolated and cloned from an E. coli gene bank. Two clones, MV12(pLC19-36) and MV12(pLC43-43), overexpressed both DMSO and trimethylamine N-oxide (TMAO) reductase activities 13- to 15-fold compared with wild-type cells. Amplification was highest in cells grown anaerobically on fumarate, while cells grown on DMSO or TMAO displayed reduced levels of enzyme amplification. Growth on nitrate or aerobic growth repressed expression of the enzyme. A 6.5-kilobase-pair DNA restriction endonuclease fragment was subcloned from pLC19-36 into the vector pBR322, yielding a recombinant DMSO reductase plasmid, pDMS159. Two polypeptides were amplified and identified on sodium dodecyl sulfate-polyacrylamide gels of proteins from E. coli HB101 harboring pDMS159: a membrane-bound protein with molecular weight 82,600 and a soluble polypeptide with molecular weight 23,600. Three plasmid-encoded polypeptides with molecular weights of 87,500, 23,300, and 22,600 were detected by in vivo transcription/translation studies. The smallest subunit was poorly defined and not detectable by Coomassie blue staining. The DMSO reductase operon was localized to the 20.0-min position on the E. coli linkage map.
Field emission from teepee-shaped carbon nanotube bundles (2004)

Carbon nanotubes were fabricated close together so that their separation is smaller than their height and field enhancement effects at the apexes of the tubes are diminished due to electrostatic shielding. Bundling was achieved by embedding the tubes into a PCVD silicon dioxide, followed by HF etching, water rinsing, and nitrogen drying. Emission measurements were performed in a vacuum system at a pressure of 4 /spl times/ 10/sup -9/ Torr. Current-voltage characteristics were obtained at room temperature, 120 /spl deg/C and 170 /spl deg/C. Macroscopic current densities of /sup 2/0 mA/cm/sup 2/ were obtained with a 0.5 mm diameter tungsten anode. The turn-on fields are about 15 V//spl mu/m.
Long‐term survival and concomitant gene expression of ribozyme‐transduced CD4+ T‐lymphocytes in HIV‐infected patients (2005)

The proof of principle results reported here demonstrate safety and feasibility of this type of gene transfer approach. While not specifically tested, T-lymphocytes containing an anti-HIV gene construct may impact on HIV-1 viral load and CD4+ T-lymphocyte count, potentially representing a new therapeutic modality for HIV-1 infection.
Intra-Specific Diversity in a Natural Population of Trifolium Repens (1980)

SUMMARY (1) The results of an investigation of the range of diversity found in a single population of the obligate outbreeding species Trifolium repens L. in North Wales are presented, together with a consideration of the ways in which the variability uncovered may be maintained in the face of potential habitat dominance by a single successful genotype replicating rapidly through clonal growth. (2) The degree of variability found within this single population for a range of biochemical, morphological and phenological characters was of a magnitude more commonly encountered in comparisons between populations taken from distinctly different environments. Nearly every clone was found to differ significantly from every other clone in at least one character. (3) The applicability of the concept of sisyphean fitness, and the suggestion that the genotypes successful in the field are those which have achieved near-maximum fitness through an interaction of a momentarily effective combination of genes and the individual's history, are considered with respect to T. repens.
Opportunistic transmission scheduling with resource-sharing constraints in wireless networks (2001)

We present an "opportunistic" transmission scheduling policy that exploits time-varying channel conditions and maximizes the system performance stochastically under a certain resource allocation constraint. We establish the optimality of the scheduling scheme and also that every user experiences a performance improvement over any nonopportunistic scheduling policy when users have independent performance values. We demonstrate via simulation results that the scheme is robust to estimation errors and also works well for nonstationary scenarios, resulting in performance improvements of 20%-150% compared with a scheduling scheme that does not take into account channel conditions. Last, we discuss an extension of our opportunistic scheduling scheme to improve "short-term" performance.
Capsaicin induces cell cycle arrest and apoptosis in human KB cancer cells (2013)

Our data demonstrate that capsaicin modulates cell cycle progression and induces apoptosis in human KB cancer cells through mitochondrial membrane permeabilization and caspase activation. These observations suggest an anti-cancer activity of capsaicin.
A New One-Step Method for the Functional Assay of the Fourth Component (C4) of Human and Guinea Pig Complement (1974)

Abstract A simple one-step method for the molecular titration of functional C4 has been developed. This method utilizes serum from the C4D strain of guinea pigs as a reagent supplying all components except C4 in relative excess. Guinea pig C4 titers obtained with this method are slightly higher than titers obtained with classical functional method. In assay of human C4, titers obtained are more than 4-fold higher than those obtained with the classical functional method. The correlation between the classical and C4D methods of C4 titration is excellent and the C4D method has less inherent variability as shown by an analysis of variance. Some of the factors which determine the end point in C4 titration by this method have been examined in detail as has been the usefulness of the method for sera of various species. The method has been used in a longitudinal study of C4 levels in nine normal subjects. In this group the range of normal titers was wide. Most, but not all, individuals showed remarkable stability in their C4 titers over long periods of time.
Seminario - Os 50 Anos da Pos-graduação no Brasil (2016)

Inauguramos esta nova secao da Revista Meta: Avaliação, com o registro dos textos base das apresentacoes feitas por ocasiao do Seminario Os 50 anos da Pos-Graduação no Brasil, realizado em 16 de dezembro de 2016, em comemoração à aprovação do Parecer no 977, de 1965, marco conceitual e regulatorio da Pos-Graduação brasileira.O Seminario foi promovido pelo Professor Carlos Alberto Serpa de Oliveira, Presidente da Fundação Cesgranrio e da Academia Brasileira de Educação. Ocorreu em 16 de dezembro de 2015, nas dependencias do Curso de Mestrado Profissional em Avaliação, no Rio de Janeiro. Contou, na Comissao Organizadora, com a participação das Professoras Maria Judith Sucupira da Costa Lins e Ligia Gomes Elliot, de Nilma Goncalves Cavalcante, Secretaria do Mestrado e egressa do Curso, Valmir Marques de Paiva, Mestrando do Curso, alem do apoio da Fundação Cesgranrio.A importância historica do distinto Relator do Parecer no 977, Professor Newton Buarque Sucupira, seu proeminente papel na Educação do pais como pensador, politico e ainda articulador da Pos-Graduação, entre outros relevantes cargos desempenhados ao longo de sua vida, e tambem a sua estreita relação com a criação da Fundação Cesgranrio, vao ser destacados nas apresentacoes daqueles que tiveram o privilegio de serem seus colegas de trabalho, alunos, orientandos e seguidores.
Effects of Long-Term Testosterone, Gonadotropin-Releasing Hormone Agonist, and Pimozide Treatments on Gonadotropin II Levels and Ovarian Development in Juvenile Female Striped Bass (Morone saxatilis)1 (1998)

The ability of the juvenile female reproductive axis to respond to hormonal stimulation was investigated in a Perciform fish, the striped bass (Morone saxatilis) using various combinations of testosterone (T), GnRH agonist (GnRHa), and pimozide. A long-term treatment with T alone, or T in combination with GnRHa, increased pituitary gonadotropin II (GtH II) levels 2- and 3-fold, respectively, suggesting that T and GnRHa each stimulate GtH II accumulation. Release of the accumulated GtH II could be induced only by high doses of GnRHa in combination with T, indicating that GtH II synthesis and release require different levels of GnRH stimulation. The addition of the dopamine antagonist pimozide did not affect pituitary and plasma GtH II levels but, in response to an additional acute GnRHa challenge, inhibited the release of GtH II. Although ovarian development was slightly stimulated by a combined T and GnRHa treatment, vitellogenesis was generally not initiated. The present study demonstrated that the juvenile striped bass pituitary is responsive to hormonal stimulation, resulting in elevated levels of GtH II in the pituitary and plasma. However, increased plasma levels of GtH II did not result in precocious puberty, suggesting that additional factors are required for the initiation of ovarian development in this teleost.
Coefficients of Coupled-Mode Equations for a Natural-Single-Phase-Unidirectional Transducer and an Electrode-Width-Difference-Reversal-of-Directivity Transducer on a 50° Y-24° X La<sub>3</sub>Ga<sub>5</sub>SiO<sub>14</sub> Substrate (2000)

Computed results of all the coefficients of coupled-mode equations are presented for a natural-single-phase-unidirectional transducer (NSPUDT) and an electrode-width-difference-reversal-of-directivity transducer on a 50° Y -24° X La 3 Ga 5 SiO 14 substrate. In order to determine all the coefficients, edge frequencies of a stop-band, standing-wave distributions at these frequencies on a substrate surface, and static capacitances of a transducer are calculated using the hybrid finite-element method. Therefore, all the effects of piezoelectric perturbation, mechanical perturbation, and energy storage due to nonradiated bulk waves are taken into account. The aluminum electrode width dependences of the coefficients are investigated in detail. Numerical results reveal that we can choose pairs of width and height of the active electrodes for a distance of λ/8 with λ being the surface acoustic wave wavelength at the center frequency between the reflection center and the transduction center. Our results of the transducer loss for NSPUDT agree well with previous experimental ones.
Considering Support and Encouragement for the Activities of Volunteer Handlers of Retired Guide Dogs (2010)

引退盲導犬飼育ボランティア活動の促進とサポートのための視点を得るために、5名の引退犬飼育ボランティアにボランティアの動機や引退犬との生活経験についてインタビューした。修正版グラウンデット・セオリー・アプローチ（ Modified Grounded Theory Approach ）による質的分析の結果、引退犬飼育過程でボランティアは＜ボランティア開始へのステップ＞、＜引退犬の立場で考えた生活＞、＜盲導犬特性の発見＞、＜ボランティア活動の意味の広がり＞、＜病と老い、死の過程に寄り添う＞という5つのカテゴリーにまとめられる体験をしていた。活動の促進とサポートとして、一般の人々が盲導犬や引退犬に触れる機会を多く提供すること、引退犬飼育は介護や看取りだけではない充実感や親密感があることを知らせること、ボランティアが訓練センターなどとの社会的連携のなかで安心して飼育出来るように相談窓口設置をすることなどが示唆された。
Tomato Transformation: Figure 1. (2008)

INTRODUCTIONTransformation is an essential technique to analyze the function of genes. Tomato can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. This protocol describes an Agrobacterium-mediated transformation method for tomato.
Generative adversarial network for denoising in dual gated myocardial perfusion SPECT using a population of phantoms and clinical data (2019)

Previously we proposed to use a generative adversarial network (GAN) in denoising dual respiratory-cardiac gating (DG) images for myocardial perfusion SPECT. In this study we further compared the use of various training datasets and demonstrated the GAN denoising effectiveness on clinical DG data. Five 4D Extended Cardiac Torso phantoms with cardiac motion, different anatomies, respiratory characteristics and activity uptakes were used in the simulation, modeling 6 respiratory and 8 cardiac gates, i.e., a total of 48 DGs. One hundred and twenty noisy LEHR projections were generated analytically and then reconstructed by the OS-EM algorithm with 6 subsets and 5 iterations. A clinical dataset for a patient who underwent SPECT/CT 1 hr post injection of 1332 MBq Tc-99m sestamibi was re-binned into 7 respiratory and 8 cardiac gates, and then reconstructed by the ML-EM algorithm with 24 iterations. The GAN was implemented using Torch. Using patients' own data, eighteen DG images were paired with the corresponding cardiac gate for training. We also evaluated the use of other patients' datasets for training by increasing the patient database from 1 to 4. The noise level measured as the normalized standard deviation (NSD) on a 2D uniform region of liver and the FWHM on the image profile drawn across the left ventricle wall were compared. In simulations, the NSD/FWHM (cm) of before and after cardiac GAN training were 0.298/1.373 and 0.083/1.489. They were 0.153/3.132, 0.118/2.646, 0.112/1.652 and 0.105/1.375 for training using one to four patients' datasets respectively. The clinical data showed that the use of GAN can lower the noise (NSD 0.246 vs 0.110) with minimal degradation of resolution (FWHM 1.682 vs 1.912). The use of patient's own data for training provide superior denoising results. More phantom and patient data are warranted to confirm our findings.
Major improvement in the detection of microsatellite instability in colorectal cancer using HSP110 T17 E-<i>ice</i>-COLD-PCR (2017)

Every colorectal cancer (CRC) patient should be tested for microsatellite instability (MSI) to screen for Lynch syndrome. Evaluation of MSI status involves screening tumor DNA for the presence of somatic deletions in DNA repeats using PCR followed by fragment analysis. While this method may lack sensitivity due to the presence of a high level of germline DNA, which frequently contaminates the core of primary colon tumors, no other method developed to date is capable of modifying the standard PCR protocol to achieve improvement of MSI detection. Here, we describe a new approach developed for the ultra-sensitive detection of MSI in CRC based on E-ice-COLD-PCR, using HSP110 T17, a mononucleotide DNA repeat previously proposed as an optimal marker to detect MSI in tumor DNA, and an oligo(dT)<sub>16</sub> LNA blocker probe complementary to wild-type genotypes. The HT17 E-ice-COLD-PCR assay improved MSI detection by 20-200-fold compared with standard PCR using HT17 alone. It presents an analytical sensitivity of 0.1%-0.05% of mutant alleles in wild-type background, thus greatly improving MSI detection in CRC samples highly contaminated with normal DNA. HT17 E-ice-COLD-PCR is a rapid, cost-effective, easy-to-implement, and highly sensitive method, which could significantly improve the detection of MSI in routine clinical testing.
Structure and Activity of V-Sn-W Oxide Catalyst for Butene Oxidation to Acetic Acid (1974)

The relationship between structure and activity of V-Sn-W oxide catalysts for butene oxidation to acetic acid was investigated, employing differential thermal analysis, I. R. -spectrometry, X-ray diffractometry and chemical analysis.An optimal calcination temperature of the catalyst was found in the range from 450 to 500°C, and the calcined catalyst was a mixture containing pure oxides of each element. The contribution of SnO2 and WO3 to the activity was thought of as promoting the reactivity of oxygen in V2O5 and as increasing the surface area of the catalyst, respectively. In the absence of oxygen, butene reduced only the V2O5 in the V-Sn-W oxide to V4O9, and in the presence of oxygen, that is, in the catalytic oxidation, the atomic ratio of V5+/VTotal(VTotal=V5++V4+) of the catalyst approached to 65% with reaction time, and there the highest activity was obtained. From these results, it is considered that activity of the catalyst is increased effectively when V2O5 and V4O9 exist simultaneously in the catalyst.Acidity of the catalyst was also determined, and it was found to be correlated to the extent of V2O5 reduction and catalytic activity.
A diet-induced animal model of non-alcoholic fatty liver disease and hepatocellular cancer (2016)

We have developed a diet-induced mouse model of non-alcoholic steatohepatitis (NASH) and hepatic cancers in a cross between two mouse strains (129S1/SvImJ and C57Bl/6J). This model mimics all the physiological, metabolic, histological, transcriptomic gene signature and clinical endpoints of human NASH and can facilitate preclinical development of therapeutic targets for NASH.
A high-efficiency tunable cw Raman oscillator (1977)

Continuously tunable cw Raman oscillation has been achieved in the visible by pumping a single-mode fused silica fiber with several lines from an argon ion laser. The 100-m-long fiber has a core diameter of 3.3 μm and a loss of 17 dB/km at 5145 Å. Threshold was achieved with ∼1.0 W of pump power in the fiber. With 4 W of 5145-Å power coupled into the fiber, the Raman oscillator was prism tuned over 80 Å. Typical linewidths were ∼2 Å. At 5280 Å, net input to output power conversion efficiencies of ∼9% and pump depletion of over 80% have been achieved. The simplicity, efficiency, and broadband tunability of fiber Raman oscillators both in the visible and in the near infrared make them potential rivals to other coherent sources of tunable radiation, such as dye lasers and parametric oscillators.
A novel smooth transition strategy for BEMF-based sensorless drive startup of PMSM (2014)

This paper proposes a novel smooth transition method, which can be applied to back electromotive force (BEMF)-based permanent magnet synchronous motors (PMSM) sensorless vector control drive. The problem with BEMF-based sensorless control of PMSM control is the inherent BEMF estimation precision before reaching a certain rotation velocity. A position open loop constant current (I-F) method, which is compared with the classical voltage-frequency (V-F) method of asynchronous motors control, is employed by applying the torque current feedback control technique to drive the motor from standstill to switch frequency. I-F strategy is analyzed first, the critical element load angle, which can influence motors switch process performance, is studied. To eliminate the switch physical parameters disturbance and achieve smooth transition, a novel strategy, that can transit the PMSM drive state from I-F, which is used during standstill and low speed rotation region, to BEMF-based sensorless FOC control smoothly, are presented. The transition strategy is furnished and its performance is shown and analyzed based on the PMSM dynamic model. At the end, an experimental platform, which is built by a high speed PMSM spindle and corresponding sensorless drive, is introduced to realize the supposed method, which can show the switch smoothness and illustrate the theoretical analysis rationality.
A dispersion compensator using coupled defects in a photonic crystal (2002)

We propose a new type of dispersion compensator that uses the characteristics of light traveling in a coupled defect waveguide (CDW) in a photonic crystal. By using a theoretical computation based on the plane-wave method, we show that the CDW band appears within the bandgap and its characteristics are well reproduced by the tight-binding (TB) model. We calculate the wavelength dispersion of light propagating in the CDW using TB formalism. The calculated result shows an inherently large dispersion of the CDW, which enables the realization of an extremely small dispersion compensator of a few tens of millimeters in size.
Achieving information flow security through monadic control of effects (2009)

This paper advocates a novel approach to the construction of secure software: controlling information flow and maintaining integrity via monadic encapsulation of effects. This approach is constructive, relying on properties of monads and monad transf
King Lear: (II) Manipulating Our Sympathies (1967)

King Lear: (II) Manipulating Our Sympathies Get access H. A. MASON H. A. MASON Search for other works by this author on: Oxford Academic Google Scholar The Cambridge Quarterly, Volume II, Issue 2, Spring 1967, Pages 148–166, https://doi.org/10.1093/camqtly/II.2.148 Published: 01 March 1967
Transient Thermal Response of the PB-AHTR to Loss of Forced Cooling (2007)

In liquid-salt cooled high temperature reactors, such as the Pebble Bed Advanced High Temperature Reactor (PB-AHTR), natural circulation of the liquid salt provides the primary decay heat removal mechanism during transients such as the loss of forced cooling (LOFC) transient. Because this natural circulation is highly effective, for LOFC the temperature differences across the core of the PB-AHTR are much lower than in modular helium reactors (MHR), where decay heat removal occurs primarily by thermal radiation and conduction. This allows the PB-AHTR to operate with much higher power density than a comparable MHR, which in turn provides the potential for attractive economics. This paper reports RELAP5-3D simulations for the PB-AHTR that verify its excellent response to LOFC transients. (authors)
A comparison of voting algorithms for n-version programming (2002)

One approach to software fault-tolerance is to develop three versions of a program from a common specification, execute these versions in parallel, and vote on the output. If two versions agree, this majority vote is taken as the correct result. Errors in a single version are thus masked out. If there is no majority, a 3-version failure occurs. In earlier experimental work, groups of versions were tested in 3- version programming mode and the resulting average failure probability compared with the average failure probability of the individual versions. This paper describes the use of 5 alternative voting algorithms and compares the resulting failure probabilities against the failure probability for standard 3-version programming. All algorithms produced an improvement over standard 3-version programming, and one algorithm was sometimes able to produce entirely correct values even when none of the three versions has done so.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The antimicrobial effect of chlorhexidine varnish on <i>mutans streptococci</i> in patients with fixed orthodontic appliances: a systematic review of clinical efficacy (2015)

A limited number of studies with low risk of bias were available to address the antimicrobial efficacy of CHX-V on MS in patients during orthodontic treatment with fixed appliances. Therefore, while the majority of studies found CHX-V to be an effective antimicrobial against MS at an interval of 3-4 weeks, the strength of the recommendation is weak. Longitudinal studies are needed to determine whether this antimicrobial effect will contribute to clinically significant caries reduction in patients undergoing orthodontic treatment with fixed appliances.
Novel metabotropic glutamate receptor 2/3 antagonists and their therapeutic applications: a patent review (2005 – present) (2014)

Recent advances in the prodrug approach of novel mGlu2/3 orthosteric antagonists combined with the design of novel mGlu2/3 and mGlu2 negative allosteric modulators provide new therapeutic opportunities for neurologic and psychiatric disorders.