gebhart/patrepeval huggingface dataset:
Detection of Single-Nucleotide Polymorphisms by PCR with Universal Energy Transfer–Labeled Primers: Application to Folate- and Cobalamin-Related Genes (2005)

Many methods for detection of single-nucleotide polymorphisms (SNPs) are based on fluorescence resonance energy transfer (1). One of these, the Amplifluor SNP genotyping system, combines the use of universal energy transfer-labeled primers (uniprimers) (2) with allele-specific amplification (3). The uniprimer is a modification of the beacon concept (1)(4); it uses the beacon’s hairpin structure, but instead of acting as a probe, the hairpin is part of the primer, and when incorporated into the amplicon, it opens and starts to fluoresce (see Fig. S1 in the Data Supplement that accompanies the online version of this Technical Brief at http://www.clinchem.org/content/vol51/issue9/) (2). The advantage of the technology is that one pair of uniprimers is used for any SNP. Several variants of the uniprimer system for SNP detection are available (5)(6)(7). We modified the method so that it became faster and less expensive and then used it to identify 3 polymorphisms related to homocysteine and B vitamins (8): transcobalamin 2 (TCN2) 776C>G, methylenetetrahydrofolate reductase (MTHFR) 677C>T, and reduced folate carrier 1 (SLC19A1) 80G>A.

For each SNP, 5 primers were used: 2 uniprimers, 2 allele-specific primers, and 1 common primer for the opposite direction. The uniprimers (Amplifluor™; Chemicon International) were labeled with either fluorescein or sulforhodamine and were provided by Flowgen. The other primers (Sigma) were designed by use of Amplifluor Assay-Architect (Serological Corporations) and Molecular Beacon Design software (Premier Biosoft International). The nonlabeled primers are listed in Table⇓ S1 in the online Data Supplement.

Purified DNA was obtained from EDTA-blood samples by use of the Wizard DNA Purification Kit (Promega Corporation). The genotype distribution was investigated in 500 EDTA-blood samples from Norwegian blood donors. For these samples, we used boiled blood supernatant as the DNA source (9). Briefly, 50 μL of whole blood was mixed with 250 …
Compensatory negotiation for agent-based project schedule coordination (2002)

The paper presents a compensatory negotiation methodology for agent based project schedule coordination; a framework wherein a project can be rescheduled dynamically by all of the concerned project participants. The compensatory negotiation methodology is developed to allow agents to transfer utility to other agents for compensation of disadvantageous agreements through a multi linked negotiation process. The multi-agent system based on this negotiation methodology will allow software assistant agents to evaluate the impact of their changes and advise the human participants.
UNDERSTANDING THE MOBILE USER’S PURCHASE-DECISION INVOLVEMENT (2015)

With smart devices starting to become truly context-aware and mobile devices establishing themselves within the on-line purchasing cycle we are finding m-commerce becoming more astute to our needs. In our efforts to facilitate this we investigate the relationship between physical and modal contexts and levels of purchase-decision involvement. We aim to increase knowledge and establish methods for increasing the level of engagement of consumers and a specific product. Previous research has shown a positive correlation between purchase-decision involvement and positive-negative user emotions. We aim to extend this by not only demonstrating that different situations can reduce or increase this correlation but that other aspects of affective state are also important when considering the mobile device user. We introduce a new term unfavourable physical situations and explore how environment stressors and level of familiarity with people and environments impact on the user’s purchase-decision involvement. Results demonstrate that extreme situations influence the relationship between different aspects of affective state and show potential in informing context-aware systems for optimising engagement with the consumer’s decision processes.
Dynamic MBSFN subframe allocation algorithm for bursty video traffic in LTE-Advanced network (2018)

The limited radio-frequency spectrum is a major problem for wireless communications, especially for the 4G and 5G systems which are expected to offer very high transmission rates. One of the most prominent solutions for this problem is to utilize efficiently the limited resources to transmit various services including the high bandwidth-consuming audio and video applications. Multimedia broadcast/multicast service (MBMS) is actually a point-to-multipoint service allowing efficient usage of network resources. The current MBMS specification uses a semi-static resource allocation mechanism for Multicast services, which is not suitable for services with Variable Bit Rate (VBR) traffic profiles, such as live video streaming. In this paper, we propose a dynamic subframe allocation algorithm which can dynamically adjust the resource allocation for MBMS transmission to adapt to the fluctuation of the VBR traffic within MBMS Single-Frequency Networks (MBSFN). The basic idea of the proposed mechanism is to reallocate periodically the MBSFN resources based on a linear prediction model of the required resources. The simulation results show that this proposed algorithm can reduce the unused MBSFN resources which could be used for unicast transmissions, without violating the fairness constraint. Moreover, we show that our algorithm outperforms the static allocation procedure in terms of unused MBSFN resources.
Concentrating solar thermal power and thermochemical fuels (2012)

Concentrated solar energy provides a virtually unlimited source of clean, non-polluting, high-temperature heat. This article reviews the underlying principles of concentrating solar radiation and describes the latest technological advances and future prospects of solar thermal power and thermochemical fuel production.
Extended conjugation in di- and tri-arylmethanes. Part I. Electronic absorption spectra of 9,9-dimethylfluorene analogues of Crystal Violet and Malachite Green (1970)

9,9-Dimethylfluorene analogues of some basic triphenylmethane dyes have been prepared and their absorption spectra have been determined. The extended quinonoid system in 9,9-dimethylfluorene is more energetically favoured than that in biphenyl, but in these dyes the mesomeric effect is not readily transmitted through the fluorenyl system.The preparations of several 9,9-dimethylfluorene intermediates are described.
Molybdenum etching with chlorine atoms and molecular chlorine plasmas (1988)

Thin films of molybdenum were etched both within and downstream from a Cl2 plasma at 200-mTorr pressure and temperatures below 180 °C. When samples were positioned downstream from the discharge, etching proceeded solely by chemical reaction of the film with chlorine atoms. Without a discharge, molecular chlorine did not etch molybdenum. Downstream and in-discharge etch rates were &lt;20 nm/min and etching by atoms was not observed below 100 °C. The chemical reaction between chlorine atoms and molybdenum was proportional to the gas phase Cl atom mole fraction.
Quantitative Extraction Using an Internally Cooled Solid Phase Microextraction Device (1995)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative Extraction Using an Internally Cooled Solid Phase Microextraction DeviceZhouyao. Zhang and Janusz. PawliszynCite this: Anal. Chem. 1995, 67, 1, 34–43Publication Date (Print):January 1, 1995Publication History Published online1 May 2002Published inissue 1 January 1995https://pubs.acs.org/doi/10.1021/ac00097a007https://doi.org/10.1021/ac00097a007research-articleACS PublicationsRequest reuse permissionsArticle Views840Altmetric-Citations271LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Three-dimensional spatiotemporal behaviors of light emission from discharge plasma of alternating current plasma display panels (2008)

Test panel for three-dimensional observation in ac plasma display panel (PDP) has been fabricated and the spatiotemporal behavior of infrared and visible emissions has been experimentally investigated in sustain discharges. Considering the infrared and visible emissions from the cathode, the cathode fall region in ac PDP could be within 70μm in thicknesses. We measured the propagation speed of the sheath plasma to be 1.8mm∕μs. In addition, the experimental result showed that an increasing sustain frequency from 100to250kHz improved the plasma efficiency.
Hydrophobic Sponge Structure‐Based Triboelectric Nanogenerator (2014)

Hydrophobic sponge structure-based triboelectric nanogenerators using an inverse opal structured film for sustainable energy harvesting over a wide range of humid atmosphere have been successfully demonstrated. The output voltage and current density reach a record value of 130 V and 0.10 mA cm(-2) , respectively, giving over 10-fold power enhancement, compared with the flat film-based triboelectric nanogenerator.
Fas and Mutant Estrogen Receptor Chimeric Gene: A Novel Suicide Vector for Tamoxifen‐inducible Apoptosis (1998)

Several cancer gene therapy strategies involve suicide genes to kill the neoplasm, or to regulate effector cells such as lymphocytes. We have developed an inducible apoptosis system with a Fas-estrogen receptor fusion protein (MfasER) for rapid elimination of transduced cells. In the present study, we further improved this molecular switch for estrogen-inducible apoptosis to overcome concerns with the wild-type estrogen receptor and its natural ligand, 17beta-estradiol (E2). The ligand-binding domain of MfasER was replaced with that of a mutant estrogen receptor which is unable to bind estrogen yet retains affinity for a synthetic ligand, 4-hydroxytamoxifen (Tm). The resultant fusion protein (MfasTmR) and MfasER were expressed in L929 cells for examination of their ligand specificities. Tm induced apoptosis in MfasTmR-expressing cells (L929MfasTmR) at 10(-8) M or higher concentrations, but induced no apoptosis in MfasER-expressing cells (L929MfasER) at up to 10(-6) M. On the other hand, E2 induced apoptosis in L929MfasER at concentrations as low as 10(-10)-10(-9) M, while it did so partially in L929MfasTmR at concentrations greater than 10(-7) M. Thus, L929MfasTmR cells were highly susceptible to Tm, but refractory to E2, with 100-1,000 times more tolerance than L929MfasER. These results suggest that the MfasTmR/Tm system would induce apoptosis in the target cells more safely in vivo, working independently of endogenous estrogen.
Multichannel Urodynamic Evaluation of Laparoscopic Burch Colposuspension for Genuine Stress Incontinence (1998)

The 2-year cure rate and multichannel urodynamics findings for laparoscopic Burch repair are similar to those reported for laparotomy. Early results of prophylactic apical vault suspension suggest that the incidence of prolapse observed after routine retropubic urethropexies may be reduced.
Evaluation of volume imaging with the HEAD PENN-PET scanner (2002)

A volume-imaging PET scanner for brain, animal, and pediatric imaging has been designed and built to achieve high performance, specifically in sensitivity and spatial resolution. The scanner is unique in its use of a single annular crystal of NaI(Tl), which allows a 30 cm diameter patient port and an axial field-of-view of 25.6 cm with a maximum axial acceptance angle a of /spl plusmn/26/spl deg/. By using a narrow photopeak energy window, this leads to high sensitivity with relatively low scatter and randoms. Image resolution is 3.5 mm in both the transverse and axial directions. Images are reconstructed with 128 slices, and are presented for phantom and patient studies (FDG) with both the multi-slice rebinning and the 3D-reprojection algorithm.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis (2003)

This is the first report to describe the release into SF of soluble molecular fragments specific for the degradation of mature, crosslinked, type II collagen (CII) in human OA and joint injury. The results provide strong evidence that the integrity of the CII network of cartilage is compromised soon after joint injury and in arthritis. This early degradation of CII may represent an important treatment target.
Super Super decoupled Loadflow (2009)

A generalized Super Super decoupled Loadflow referred to as SSDL-X'X' method is presented along with its special case manifestations leading to the proposed SSDL-YY method. Gain matrices of the SSDL-YY method are defined independent of rotation angles and they differ only in their diagonal terms. The diagonal terms in the gain matrix of Q-V sub-problem are characterized by the presence of transformed specified/known quantities. Rotation angles are limited to the maximum value of -48°, and the slack-start as a guess solution is suggested for increased efficiency. These along with simple modification of real power mismatches at PV-nodes yields a robust and fast loadflow method. An advanced version of the General-purpose Fast Decoupled Loadflow (GFDL) is manifested as SSDL-BX method. The proposed method is tested and compared with Fast Super Decoupled Loadflow (FSDL), SSDLBX, and GFDL methods under wide range of simulated system operating conditions and network parameters. The proposed method is superior to the FSDL method under all most all conditions and can be more than twice faster than the SSDL-BX, and GFDL methods for systems having large R/X ratio branches.
The effects of desipramine and iprindole on levels of enantiomers of fluoxetine in rat brain and urine (1994)

The antidepressant fluoxetine (FLU) and its N-demethylated metabolite, norfluoxetine (NFLU), each contains a chiral center. The combination of FLU and desipramine (DMI), another antidepressant, has been reported to be useful in treatment of depression, to dramatically increase plasma levels of DMI and also to produce more rapid beta-adrenergic receptor down-regulation in brain than caused by DMI alone. We have now begun studies on the effects of this drug combination on the levels of FLU and NFLU enantiomers in the rat. In addition, the combination of FLU and iprindole (IPR) was also investigated. Male Sprague-Dawley rats were treated intraperitoneally with either normal saline vehicle, DMI (5 mg/kg/day), (R,S)-FLU (10 mg/kg/day) or DMI (5 mg/kg/day) + (R,S)-FLU (10 mg/kg/day) for 4 days. Following the last treatment, 24 h urine samples were collected. Rats were sacrificed and brains were removed. For the IPR study, rats were pretreated with either saline or IPR-HCl (11.2 mg/kg) and then treated 1 h later with (R,S)-FLU. After 5 h, the rats were sacrificed and brains were removed. Brain and urine samples were analyzed by gas chromatography with electron-capture detection for free (R)-and (S)-FLU and (R)- and (S)-NFLU after extraction and reaction with (-)-(S)-N-(trifluoroacetyl)prolyl chloride. The results from the brains of the rats treated with DMI/FLU indicate that levels of enantiomers of both FLU and NFLU were significantly increased over those seen in the animals receiving (R,S)-FLU alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Collaboration in Deep-sea Biodiversity - Expanding the value from Deepwater E&P Operations (2004)

Abstract Oil and Gas Exploration relies heavily upon the use of Remotely Operated Vehicles and data collection in many deep-water production areas. Through collaboration projects between scientific instiutions and production companies it has been possible to gain great ecological value from existing data and new data routinely collected as part of normal offshore exploration activities.
Pharmacological Effects of the Spin Trap Agents<i>N</i>-t-Butyl-Phenylnitrone (PBN) and 2,2,6,6-Tetramethylpiperidine-<i>N</i>-Oxyl (TEMPO) in a Rabbit Thromboembolic Stroke Model (2001)

This study suggests that certain spin trap agents may have deleterious effects when administered after an embolic stroke. However, spin trap agents such as PBN or TEMPO, when administered in combination with tPA, may improve the safety of tPA by reducing the incidence of tPA-induced hemorrhage. Overall, the therapeutic benefit of spin trap agents for the treatment of ischemic stroke requires additional scrutiny before they can be considered "safe" therapeutics.
Glass‐Forming Systems Involving GeO <sub>2</sub> with Bi <sub>2</sub> O <sub>3</sub> , Ti <sub>2</sub> O, and PbO (1982)

The previously studied system GeO 2 ‐Bi 2 O 3 ‐TI 2 O was extended with the addition of PbO using air‐ and water‐quenched melted samples. Large areas of glass formation were found in the systems GeO 2 –Bi 2 O 3 –PbO and GeO 2 –PbO–Tl 2 O at all but the lowest GeO 2 contents. Glasses were examined by powder X‐ray diffraction, differential thermal analysis, thermomechanical analysis, and Archimedes’technique to obtain glass transition and crystallization exotherm temperatures, thermal expansion coefficients, and densities, which are presented in diagrams for the GeO 2 ‐PbO binary and for the two ternary systems. Based on calculated values of λ 0 , the wavelength for zero material dispersion, compositions in this system may be useful for construction of ultralow‐loss optical waveguides in the μm region.
Molecular Characterization of Antigen-Peptide Pulsed Dendritic Cells: Immature Dendritic Cells Develop a Distinct Molecular Profile when Pulsed with Antigen Peptide (2014)

As dendritic cells (DCs) are the most potent professional antigen-presenting cells, they are being tested as cancer vaccines for immunotherapy of established cancers. Although numerous studies have characterized DCs by their phenotype and function, few have identified potential molecular markers of antigen presentation prior to vaccination of host. In this study we generated pre-immature DC (piDC), immature DC (iDC), and mature DC (mDC) from human peripheral blood monocytes (PBMC) obtained from HLA-A2 healthy donors, and pulsed them with human papillomavirus E7 peptide (p11-20), a class I HLA-A2 binding antigen. We then characterized DCs for cell surface phenotype and gene expression profile by microarray technology. We identified a set of 59 genes that distinguished three differentiation stages of DCs (piDC, iDC and mDC). When piDC, iDC and mDC were pulsed with E7 peptide for 2 hrs, the surface phenotype did not change, however, iDCs rather than mDCs showed transcriptional response by up-regulation of a set of genes. A total of 52 genes were modulated in iDC upon antigen pulsing. Elongation of pulse time for iDCs to 10 and 24 hrs did not significantly bring further changes in gene expression. The E7 peptide up-modulated immune response (KPNA7, IGSF6, NCR3, TREM2, TUBAL3, IL8, NFKBIA), pro-apoptosis (BTG1, SEMA6A, IGFBP3 and SRGN), anti-apoptosis (NFKBIA), DNA repair (MRPS11, RAD21, TXNRD1), and cell adhesion and cell migration genes (EPHA1, PGF, IL8 and CYR61) in iDCs. We confirmed our results by Q-PCR analysis. The E7 peptide but not control peptide (PADRE) induced up-regulation of NFKB1A gene only in HLA-A2 positive iDCs and not in HLA-A2 negative iDCs. These results suggest that E7 up-regulation of genes is specific and HLA restricted and that these genes may represent markers of antigen presentation and help rapidly assess the quality of dendritic cells prior to administration to the host.
Record Capture and Acceleration Efficiency in the SURF-II 300 MeV Circular Storage Ring (1989)

SURF-II is a 300-MeV, single-magnet, weak-focusing, circular storage ring. It is dedicated to the production of VUV (vacuum ultraviolet) and soft X-ray radiation for research and for absolute radiometry. The injector is a 10-MeV microtron. A single output pulse from the microtron is injected into the storage ring (SR) and accelerated to operating energy., The microtron delivers 1- mu s-long, 80-mA pulses. With multiturn capture, more than 300 mA have been accelerated to full energy. On March 13, 1989, the average beam current was 262 mA and every beam was greater than 250 mA. This capture and acceleration efficiency is being achieved by improved transport line optics, the kicker magnet pulse, the SR's magnetic field gradient, and the RF system. >
Challenges in the design of frequency synthesizers for wireless applications (2002)

This paper describes the challenges in the design of frequency synthesizers used in wireless transceivers. Following a review of design issues and the effect of nonidealities, we present a number of synthesizer architectures along with their merits and drawbacks. We also describe the difficulties in the design of some of the building blocks and consider the role of synthesizers in emerging applications.
Starch Granule Initiation in<i>Arabidopsis</i>Requires the Presence of Either Class IV or Class III Starch Synthases (2009)

The mechanisms underlying starch granule initiation remain unknown. We have recently reported that mutation of soluble starch synthase IV (SSIV) in Arabidopsis thaliana results in restriction of the number of starch granules to a single, large, particle per plastid, thereby defining an important component of the starch priming machinery. In this work, we provide further evidence for the function of SSIV in the priming process of starch granule formation and show that SSIV is necessary and sufficient to establish the correct number of starch granules observed in wild-type chloroplasts. The role of SSIV in granule seeding can be replaced, in part, by the phylogenetically related SSIII. Indeed, the simultaneous elimination of both proteins prevents Arabidopsis from synthesizing starch, thus demonstrating that other starch synthases cannot support starch synthesis despite remaining enzymatically active. Herein, we describe the substrate specificity and kinetic properties of SSIV and its subchloroplastic localization in specific regions associated with the edges of starch granules. The data presented in this work point to a complex mechanism for starch granule formation and to the different abilities of SSIV and SSIII to support this process in Arabidopsis leaves.
Non-contact acoustic radiation force impulse microscopy via photoacoustic detection for probing breast cancer cell mechanics (2014)

We demonstrate a novel non-contact method: acoustic radiation force impulse microscopy via photoacoustic detection (PA-ARFI), capable of probing cell mechanics. A 30 MHz lithium niobate ultrasound transducer is utilized for both detection of phatoacoustic signals and generation of acoustic radiation force. To track cell membrane displacements by acoustic radiation force, functionalized single-walled carbon nanotubes are attached to cell membrane. Using the developed microscopy evaluated with agar phantoms, the mechanics of highly- and weakly-metastatic breast cancer cells are quantified. These results clearly show that the PA-ARFI microscopy may serve as a novel tool to probe mechanics of single breast cancer cells.