gebhart/patrepeval huggingface dataset:
Trehalose synthase of <i>Mycobacterium smegmatis</i> (2004)

Trehalose synthase (TreS) catalyzes the reversible interconversion of trehalose (glucosyl-alpha,alpha-1,1-glucose) and maltose (glucosyl-alpha1-4-glucose). TreS was purified from the cytosol of Mycobacterium smegmatis to give a single protein band on SDS gels with a molecular mass of approximately 68 kDa. However, active enzyme exhibited a molecular mass of approximately 390 kDa by gel filtration suggesting that TreS is a hexamer of six identical subunits. Based on amino acid compositions of several peptides, the treS gene was identified in the M. smegmatis genome sequence, and was cloned and expressed in active form in Escherichia coli. The recombinant protein was synthesized with a (His)(6) tag at the amino terminus. The interconversion of trehalose and maltose by the purified TreS was studied at various concentrations of maltose or trehalose. At a maltose concentration of 0.5 mm, an equilibrium mixture containing equal amounts of trehalose and maltose (42-45% of each) was reached during an incubation of about 6 h, whereas at 2 mm maltose, it took about 22 h to reach the same equilibrium. However, when trehalose was the substrate at either 0.5 or 2 mm, only about 30% of the trehalose was converted to maltose in >or= 12 h, indicating that maltose is the preferred substrate. These incubations also produced up to 8-10% free glucose. The K(m) for maltose was approximately 10 mm, whereas for trehalose it was approximately 90 mm. While beta,beta-trehalose, isomaltose (alpha1,6-glucose disaccharide), kojibiose (alpha1,2) or cellobiose (beta1,4) were not substrates for TreS, nigerose (alpha1,3-glucose disaccharide) and alpha,beta-trehalose were utilized at 20 and 15%, respectively, as compared to maltose. The enzyme has a pH optimum of about 7 and is inhibited in a competitive manner by Tris buffer. [(3)H]Trehalose is converted to [(3)H]maltose even in the presence of a 100-fold or more excess of unlabeled maltose, and [(14)C]maltose produces [(14)C]trehalose in excess unlabeled trehalose, suggesting the possibility of separate binding sites for maltose and trehalose. The catalytic mechanism may involve scission of the incoming disaccharide and transfer of a glucose to an enzyme-bound glucose, as [(3)H]glucose incubated with TreS and either unlabeled maltose or trehalose results in formation of [(3)H]disaccharide. TreS also catalyzes production of a glucosamine disaccharide from maltose and glucosamine, suggesting that this enzyme may be valuable in carbohydrate synthetic chemistry.
Monoglucose Derivatives of Gentisic Acid (1964)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMonoglucose Derivatives of Gentisic AcidA. Zane and S. H. WenderCite this: J. Org. Chem. 1964, 29, 7, 2078–2079Publication Date (Print):July 1, 1964Publication History Published online1 May 2002Published inissue 1 July 1964https://pubs.acs.org/doi/10.1021/jo01030a547https://doi.org/10.1021/jo01030a547research-articleACS PublicationsRequest reuse permissionsArticle Views144Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Identification of Components of Osage Orange Fruit (<i>Maclura pomifera</i>) and Their Repellency to German Cockroaches (2002)

Abstract The fruit of the Osage orange tree (Maclura pomifera (Raf.) Schnied.) was extracted by several methods: solid phase microextraction (SPME) volatile trapping of the headspace, solvent extraction with hexane, soxhlet extraction with hexane or methylene chloride and steam distillation. The isolates from each technique were subjected to GC/MS to compare extract components. Ripe and unripe fruits were compared using SPME. Several compounds of terpenoid structure, mostly sesquiterpenoids, were determined as present in the isolates. Among these were elemol/hedycaryol, α-copaene, α-cubebene, δ-cadinene, β-elemene, β-caryophyllene, α-ylangene/valencene, (Z,E)-farnesol and the green volatile hexyl hexanoate. The behavioral response of German cockroaches [Blattella germanica (L.)] to the oil and extracts was examined in a choice arena, and the extracts were found to be repellent. Individual compounds, including the isoflavones osajin and pomiferin, were purchased or isolated and tested directly, and several were found to be repellent to the cockroaches. Key Word Index: Madura pomifera MoraceaeOsage orangeessential oil compositionextract compositionheadspace analysiselemol/hedycaryolα-cubebeneγ-cadineneβ-elemene(Z,E)-farnesolhexyl hexanoateosajinpomiferin Blattella germanica cockroach repellency
Single-crystalline CdTe nanowire field effect transistors as nanowire-based photodetector (2014)

The electronic and photoconductive characteristics of CdTe nanowire-based field effect transistors were studied systematically. The electrical characterization of a single CdTe nanowire FET verifies p-type behavior. The CdTe NW FETs respond to visible-near infrared (400–800 nm) incident light with a fast, reversible and stable response characterized by a high responsivity (81 A W−1), photoconductive gain (∼2.5 × 104%) and reasonable response and decay times (0.7 s and 1 s, respectively). These results substantiate the potential of CdTe nanowire-based photodetectors in optoelectronic applications.
Acoustic lance: New <i>in</i> <i>situ</i> seafloor velocity profiles (1996)

The acoustic lance is an instrument developed to obtain in situ compressional wave velocity and attenuation (Q−1) profiles for a sedimentary layer of several meters thickness at the sediment–seawater interface. The self-contained instrument consists of ten independent recording channels with a linear array of receivers embedded in the seafloor below a broadband acoustic source. It provides in situ recording of full waveforms to determine interval velocity and attenuation. The system can be attached to a gravity corer or to a specially designed probe. A comprehensive experiment was carried out in Mid-Atlantic Ridge sediment ponds where the lance made in situ measurements, and core samples were recovered. Core data agree well with in situ data in one location, but disagree in other locations. Lance data indicate that the sediment ponds have similar in situ velocity distributions, with an acoustic channel much thinner than that predicted by earlier investigators.
Optimization design method of throttle slice thickness of telescopic shock absorber (2009)

Throttle slice thickness is the key factor that affects the characteristic of telescope-damper, but there is no any accurate and reliable design method. This paper analyzed the various damping factors which affect the design of throttle slice thickness, and the mathematics model that for throttle slice thickness by single velocity value is found with the analytic formula of throttle slice deformation and the relation formula of pressure with flux. Based on this, the target function of curve fitting optimization design for throttle slice thickness is established. A practical design example of throttle slice thickness of telescope-damper is given, the designed values is compared with that by single velocity and shown on the telescope-damper blueprint of factory. The performance test was conducted for the telescope-damper developed with the new design methods, and compared with the desired values, the tested values approach that desired. The experiment results show that the method of curve fitting optimization design for the throttle slice thickness is accurate, and the designed value is reliable and precision.
Association between diabetes, family history of diabetes, and risk of nonalcoholic steatohepatitis and fibrosis (2012)

Diabetes is strongly associated with risk of NASH, fibrosis, and advanced fibrosis. Family history of diabetes, especially among nondiabetics, is associated with NASH and fibrosis in NAFLD.
Improved magnetic voltage stabilizer employing silicon-controlled rectifiers (1961)

This paper will describe an improved stabilizer illustrated in Figure 1. Inductor L and capacitor C are the same as in conventional stabilizers, while the heavy saturable reactor is replaced as follows:
Los obreros en la Revolución mexicana: Los "Batallones Rojos" (1971)

-
Serving Justice: How to Give a Deposition (1991)

1Barbarn Jo Anderson, RN, MS, is clinical care coordinator of the Head Injury Treatment Programs of the Hillhaven Corporation, Lexington, MA. 2Niki Ayers, RN, MS, CS, is medical/gerontogical clinilcal nurse specialist, University Hospital, Boston, MA. 3Alexandra B. Harvey, Esq., is an associate at the litigation firm Taylor, Anderson, and Travers, Boston
Daylight fluorescent pigments and their applications (1978)

Since the earliest man first started to decorate cave walls with coloured materials produced from earth pigments and vegetable binders, colour has experienced the most profound influence on contemporary living.
Efficient Labeling Mechanism for Dynamic XML Document Trees (2010)

There are two main problems about the existing labeling mechanism: low space-time efficiency and insufficient supporting for dynamic update of XML document.Combining with the prime and IBSL labeling mechanism,this paper presents a novel XML tree labeling called Prime-based Binary String Labeling(PBSL),which has high efficiency in query,can support update operation for XML document,and greatly reduce the storage space.
<title>Fiber optic ice sensors for refrigerators</title> (1991)

In any kind of refrigerator, frost and ice formation has to be limited by defrosting of the evaporator surface. This paper proposes the use of fiber-optic sensor technology to successfully achieve the goal of sensing and controlling ice formation inside refrigerators, by monitoring ice thickness and servocontrolling the defrosting cycle. The main advantages of such a choice are discussed. Different fiber optic sensors are analyzed. An experimental investigation on a test bench and inside a real refrigerator is performed. The problem of cost of the sensor is also addressed.
Introduction (1996)

I am very concerned about the careless use of words such as 'care', 'counselling', 'service' and 'empowerment', and a cluster of similar expressions that are now being applied, and abused, everywhere. I want to show how this has led to sloppy thinking, misplaced emotion and expectation, and sheer nonsense in care sector markets. Nowhere is this worse, I believe, than in the particular growth areas of counselling and psychotherapy, variations of which are springing up everywhere. These will be the main focus of attention in the following pages, but an understanding of the booming growth of counselling, and counsellor training, requires a glance at the kind of society and economy that has created these supposedly new forms of care.
Lossless compression of VLSI layout image data (2006)

We present a novel lossless compression algorithm called Context Copy Combinatorial Code (C4), which integrates the advantages of two very disparate compression techniques: context-based modeling and Lempel-Ziv (LZ) style copying. While the algorithm can be applied to many lossless compression applications, such as document image compression, our primary target application has been lossless compression of integrated circuit layout image data. These images contain a heterogeneous mix of data: dense repetitive data better suited to LZ-style coding, and less dense structured data, better suited to context-based encoding. As part of C4, we have developed a novel binary entropy coding technique called combinatorial coding which is simultaneously as efficient as arithmetic coding, and as fast as Huffman coding. Compression results show C4 outperforms JBIG, ZIP, BZIP2, and two-dimensional LZ, and achieves lossless compression ratios greater than 22 for binary layout image data, and greater than 14 for gray-pixel image data.
Filter Selection for Multispectral Color Image Acquisition (2004)

The quality of a multispectral color image acquisition system depends on many factors, the spectral sensitivity of the different channels being one of them. In a relatively common setup, a multispectral camera is being implemented by coupling a monochrome digital camera with a set of optical filters, typically mounted on a filter wheel. The properties of these filters is an important component of the system design. Different methods have been proposed for the design or selection of appropriate filters. In this article we review several methods used for selection of an optimal subset of filters from a set of available filters. The different filter selection methods are subjected to a comprehensive evaluation procedure, in which their quality is evaluated mainly in terms of the ability of the resulting system to reconstruct scene spectral reflectances.
The mTOR kinase inhibitor Everolimus decreases S6 kinase phosphorylation but fails to reduce mutant huntingtin levels in brain and is not neuroprotective in the R6/2 mouse model of Huntington's disease (2010)

Our data suggests that beneficial behavioral effects of everolimus in R6/2 mice result primarily from effects on muscle. Even though everolimus significantly modulated its target brain S6 kinase, this did not decrease mutant huntingtin levels or provide neuroprotection.
Roles of Gangliosides in Hypothalamic Control of Energy Balance: New Insights (2020)

Gangliosides are essential components of cell membranes and are involved in a variety of physiological processes, including cell growth, differentiation, and receptor-mediated signal transduction. They regulate functions of proteins in membrane microdomains, notably receptor tyrosine kinases such as insulin receptor (InsR) and epidermal growth factor receptor (EGFR), through lateral association. Studies during the past two decades using knockout (KO) or pharmacologically inhibited cells, or KO mouse models for glucosylceramide synthase (GCS; <i>Ugcg</i>), GM3 synthase (GM3S; <i>St3gal5</i>), and GD3 synthase (GD3S; <i>St8sia1</i>) have revealed essential roles of gangliosides in hypothalamic control of energy balance. The a-series gangliosides GM1 and GD1a interact with leptin receptor (LepR) and promote LepR signaling through activation of the JAK2/STAT3 pathway. Studies of GM3S KO cells have shown that the extracellular signal-regulated kinase (ERK) pathway, downstream of the LepR signaling pathway, is also modulated by gangliosides. Recent studies have revealed crosstalk between the LepR signaling pathway and other receptor signaling pathways (e.g., InsR and EGFR pathways). Gangliosides thus have the ability to modulate the effects of leptin by regulating functions of such receptors, and by direct interaction with LepR to control signaling.
Application of data mining techniques to identify structural congestion problems under uncertainty (2002)

This paper proposes a novel methodology to identify congestion problems under both "traditional" and "new" uncertainties such as generation costs, location and size of new generators, retirement of old ones, generation patterns, etc. The methodology allows not only identifying the transmission paths and corridors which will have congestion problems, but also the scenarios producing these critical situations. Thus, it can be used not only to simplify the study of new investments (reinforcement of existing lines), but also to facilitate the evaluation of hedging strategies and the design of proactive policies to avoid the detected congestion.
HIP diffusion bonding of FGH96-DD6 dual alloys (2014)

Diffusion bonding of powder metallurgy superalloy FGH96 to single crystal superalloy DD6 by hot iso-static pressing (HIP) was studied. The microstructure and elements diffusion at FGH96-DD6 interface were discussed. The influence of HIP process on the microstructure and mechanical properties of FGH96 and DD6 was investigated. Tensile tests of FGH96-DD6 diffusion joints performed at 750°C showed that metallurgical diffusion bonding of FGH96 to DD6 could be achieved by the appropriate HIP process. The γ′ phase of DD6 grew up with increase of HIP temperature. The tensile mechanical properties of DD6 could also be maintained up to the desired levels with the appropriate HIP process. For FGH96, the variation of HIP temperature in the experimental range did not affect obviously the tensile mechanical property at 750°C.
Pathogenic role of Fgf23 in <i>Hyp</i> mice (2006)

Inactivating mutations of the PHEX (phosphate-regulating gene with homologies to endopeptidases on the X chromosome) endopeptidase, the disease-causing gene in X-linked hypophosphatemia (XLH), results in increased circulating levels of fibroblastic growth factor-23 (FGF23), a bone-derived phosphaturic factor. To determine the causal role of FGF23 in XLH, we generated a combined Fgf23-deficient enhanced green fluorescent protein (eGFP) reporter and Phex-deficient Hyp mouse model (Fgf23(+/-)/Hyp). eGFP expression was expressed in osteocytes embedded in bone that exhibited marked upregulation of eGFP in response to Phex deficiency and in CD31-positive cells in bone marrow venules that expressed low eGFP levels independently of Phex. In bone marrow stromal cells (BMSCs) derived from Fgf23(-/-)/Hyp mice, eGFP expression was also selectively increased in osteocyte-like cells within mineralization nodules and detected in low levels in CD31-positive cells. Surprisingly, eGFP expression was not increased in cell surface osteoblasts, indicating that Phex deficiency is necessary but not sufficient for increased Fgf23 expression in the osteoblast lineage. Additional factors, associated with either osteocyte differentiation and/or extracellular matrix, are necessary for Phex deficiency to stimulate Fgf23 gene transcription in bone. Regardless, the deletion of Fgf23 from Hyp mice reversed the hypophosphatemia, abnormal 1,25(OH)(2)D(3) levels, rickets, and osteomalacia associated with Phex deficiency. These results suggest that Fgf23 acts downstream of Phex to cause both the renal and bone phenotypes in Hyp mice.
User-managed access to web resources (2010)

Web 2.0 technologies have made it possible to migrate traditional desktop applications to the Web, resulting in a rich and dynamic user experience and in expanded functionality. Individuals can create and manage their content online, and they are not only consumers of Web services, but also active participants on the Web platform. As a result, potentially large amounts of personal, sensitive, and valuable data is put online, spread across various Web services. Users sometimes share this data with other users and services on the Web, but are also concerned about maintaining privacy and sharing their data securely.
Time Course of <i>de Novo</i> Adipogenesis in Matrigel by Gelatin Microspheres Incorporating Basic Fibroblast Growth Factor (2002)

Controlled release of basic fibroblast growth factor (bFGF) from gelatin microspheres achieved de novo adipogenesis at the implanted site of a basement membrane extract (Matrigel). Following subcutaneous co-implantation of Matrigel and gelatin microspheres incorporating 0.1 microg of bFGF into the back of mice, adipose tissue was formed at the implanted site after 4 weeks postoperatively although the extent increased with implantation time. Formation of adipose tissue was significantly faster than the co-implantation of Matrigel, and 0.1 microg of free bFGF while a larger volume of the adipose tissue formed was retained 15 weeks later. When measured in Matrigel co-implanted with the gelatin microspheres incorporating bFGF, the number of cells infiltrated into Matrigel increased to a significantly high extent compared with the bFGF co-implantation. Matrigel alone was much less effective in inducing formation of adipose tissue. We conclude that gelatin microspheres incorporating bFGF enable Matrigel to efficiently induce de novo adipogenesis at the implanted site in respect to the formation rate and volume of adipose tissue.
FOLLOWING IN ZOLA'S FOOTSTEPS, London, 26 September 1993 (1994)

FOLLOWING IN ZOLA'S FOOTSTEPS, London, 26 September 1993 Get access The Emile Zola Society is based at the Institut Francais, 17 Queensbury Place, London SW72DT 071 5896211. Secretary Chantal Morel. 1992. ISBN 071 5896211. JO STANLEY JO STANLEY Search for other works by this author on: Oxford Academic Google Scholar History Workshop Journal, Volume 37, Issue 1, SPRING 1994, Pages 239–240, https://doi.org/10.1093/hwj/37.1.239 Published: 01 March 1994
Connections between the processing and nuclear export of mRNA: Evidence for an export license? (2000)

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
The PDB data uniformity project (2001)

The Protein Data Bank (PDB; http://www.rcsb.org/pdb/) is the single worldwide archive of structural data of biological macromolecules. This paper describes the data uniformity project that is underway to address the inconsistency in PDB data.
Building Classifiers to Identify Split Files. (2009)

Abstract. We apply machine-learning techniques to help automate the process of mining the version history of software projects. Analysis of version histories is important in the study of software evolution. One of the associated problems is tracing program elements which have changed or moved as the result of file restructuring. As an initial application, we have developed classifiers to identify one such type of file change, ‘split files’. Our process involves extracting features through syntactic analysis of the original source code, and then training and evaluating classifiers against a set of data assessed by visual inspection. We analysed 266K files from 84 open-source projects, filtering out a set of candidate files for which our classifiers achieve either 89 % overall accuracy, or a false positive rate of 5%.
Generation of near-infrared light pulses from ZnS:Cr under laser-enhanced cathode-beam excitation (1995)

The method of additional heating of a tungsten hair-pin cathode by laser pulses for generating strong and short electron-beam pulses is applied to cathodoluminescence measurements with the aim of examining effects of high excitation densities. A change in the risetime of the Cr2+(d4) luminescence at different current densities is observed in ZnS. The mechanism of this effect is discussed.