gebhart/patrepeval huggingface dataset:
Alloantigen-specific suppressor T cells are not inhibited by cyclosporin A, but do require IL 2 for activation. (1986)

Alloantigen-specific suppressor T cells are activated from normal murine spleen cells in mixed lymphocyte reactions (MLR). These T cells are radioresistant and suppress the activation of cytotoxic T lymphocytes (CTL) in second primary MLR cultures. This report demonstrates that cyclosporin A (CsA) blocks the activation of these suppressor cells at a dose of 1 microgram/ml. However, reconstitution of CsA blocked cultures with IL 2 restores the activation of the suppressor T cells, but fails to significantly restore the activation of CTL in these same cultures. This differential activation requirement was used to establish T cell lines that demonstrate enriched suppressor cell activity but depletion of CTL activity. These findings are discussed in terms of the mechanism of action of CsA in these distinct T cell subsets and the relevance to models of allograft unresponsiveness.
Resonant tunneling device with multiple negative differential resistance: Digital and signal processing applications with reduced circuit complexity (1987)

A new approach to obtain multiple peaks in the current-voltage characteristic of a resonant-tunneling (RT) device is demonstrated. The peaks are generated using only the ground state resonance of the quantum well rather than several states, as in conventional RT devices. The separation between the peaks is voltage tunable and also the peak currents can be made nearly equal, which is necessary to use the device in a variety of circuit applications. A functional device operating at 100 K, with two peaks in the I-V has been fabricated. The first practical demonstration of circuits for frequency multiplication by a factor of five, a three-state memory and a 4-bit parity generator, using a single functional RT device each, is also reported. The use of multiple-peak RT devices in these circuits results in an order of magnitude reduction in the number component per function over conventional techniques.
Prosthetic Rehabilitation of a Patient with Gastroesophageal Reflux Disease: 4-Year Followup (2014)

The gastroesophageal reflux disease (GERD) is one of the main causes of dental erosion. The aim of this case presented is to describe the prosthetic rehabilitation of a patient with GERD after 4 years of followup. A 33-year-old male patient complained about tooth sensitivity. The lingual surface of the maxillary anterior teeth and the cusps of the upper and lower posterior teeth presented wear. It was suspected that the feeling of heartburn reported by the patient associated with the intake of sports supplements (isotonics) was causing gastroesophageal changes. The patient was referred to a gastroenterologist and was diagnosed with GERD. Dental treatment was performed with metal-free crowns and porcelain veneers after medical treatment of the disease. With the change in eating habits, the treatment of GERD and lithium disilicate ceramics provided excellent cosmetic results after 4 years and the patient reported satisfaction with the treatment.
Dose response to inhaled salbutamol in chronic obstructive airways disease (1991)

High dose inhaled salbutamol is increasingly used in the management of chronic obstructive airways disease. To determine the range of doses to achieve optimal bronchodilatation and the proportion of patients requiring high dose therapy we have studied 23 patients with chronic obstructive airways disease. Cumulative dose responses were measured to six incremental doses of salbutamol (0.2 to 1.2 mg) delivered by metered dose inhaler. Results were analysed by polynomial regression to calculate the smallest dose required to produce 90% maximal bronchodilatation in each patient. While 5/23 (22%) required greater than 1 mg the majority, 14/23 (61%), achieved 90% maximal bronchodilation with salbutamol 0.6 mg or less. The 8 patients with severe airflow limitation (FEV1 less than or equal to 1 litre) showed a similar pattern of response. We conclude that in chronic obstructive airways disease there are wide individual variations in the dose of inhaled salbutamol producing 90% maximal bronchodilatation with only a minority requiring high dose therapy.
Microstructural Transformations of an Amorphous Fe<sub>90</sub> Zr<sub>10</sub> Alloy Induced by High-Energy Ball-Milling (2006)

This DOI is not currently attached to any metadata records. DOIs can’t actually ever be deleted (they’re persistent), but sometimes our members create DOIs in error. We do have a process to approximate deletion which we follow only in rare cases where the DOI has been genuinely created in error, and most crucially, if the DOI has never been published anywhere online or in print and never otherwise distributed to or communicated with anyone (authors, readers, reviewers, etc.
Chemistry of isoprenylated cysteinyl containing peptides. [2,3]. Sigmatropic rearrangement of S-farnesylcysteinyl sulfoxides. Studies toward a mild method of deprenylating lipopeptides. (1992)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChemistry of isoprenylated cysteinyl containing peptides. [2,3]. Sigmatropic rearrangement of S-farnesylcysteinyl sulfoxides. Studies toward a mild method of deprenylating lipopeptides.Eric M. Gordon and Jelka PluscecCite this: J. Am. Chem. Soc. 1992, 114, 4, 1521–1523Publication Date (Print):February 1, 1992Publication History Published online1 May 2002Published inissue 1 February 1992https://pubs.acs.org/doi/10.1021/ja00030a081https://doi.org/10.1021/ja00030a081research-articleACS PublicationsRequest reuse permissionsArticle Views171Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Kinetic Studies of Lysine-Sensitive Aspartate Kinase Purified from Maize Suspension Cultures (1990)

Steady state substrate kinetics and feedback regulation properties were determined for lysine-sensitive aspartate kinase (AK) purified from Black Mexican Sweet maize (Zea mays L.) cell suspension cultures. Two AK isoforms (AK Early and AK Late) were separated by two passages through an anion exchange column as the final steps in a procedure giving 1200-fold purification. Kinetic properties were determined for the major AK Late eluting isoform. Assays were conducted at the pH activity maximum (8.0) and with excess Mg(2+) to favor a two-substrate reaction involving aspartate and complexed MgATP. AK catalyzed a sequential reaction in which MgATP and aspartate both bind to the enzyme complex before the ADP and aspartyl-phosphate products are released. The K(m) value calculated for MgATP was 0.43 millimolar and for aspartate was 1.04 millimolar. Cooperativity in substrate binding was not observed and was not induced by lysine. The lysine concentration required for 50% inhibition of AK activity was 7 micromolar. An apparent Hill coefficient of 1.4 indicated a minimum of two lysine-binding sites on the active AK complex. At nonsaturating substrate concentrations, lysine inhibition was characteristic of an S-parabolic, I-parabolic noncompetitive allosteric inhibitor. The parabolic inhibitor replot, Hill coefficients > 1, and the lack of substrate cooperativity were consistent with a model for multiple lysine-binding sites per active AK subunit. Similar kinetic properties were observed for the AK Early isoform.
Jim Campbell (2005)

Article Jim Campbell Share on Author: Jim Campbell San Francisco, California San Francisco, CaliforniaView Profile Authors Info & Claims SIGGRAPH '05: ACM SIGGRAPH 2005 Electronic Art and Animation CatalogAugust 2005 Pages 10–13https://doi.org/10.1145/1086057.1086062Online:01 August 2005Publication History 3citation351DownloadsMetricsTotal Citations3Total Downloads351Last 12 Months1Last 6 weeks0 Get Citation AlertsNew Citation Alert added!This alert has been successfully added and will be sent to:You will be notified whenever a record that you have chosen has been cited.To manage your alert preferences, click on the button below.Manage my AlertsNew Citation Alert!Please log in to your account Save to BinderSave to BinderCreate a New BinderNameCancelCreateExport CitationPublisher SiteGet Access
Statistical Analysis of Molecular Signal Recording (2013)

A molecular device that records time-varying signals would enable new approaches in neuroscience. We have recently proposed such a device, termed a "molecular ticker tape", in which an engineered DNA polymerase (DNAP) writes time-varying signals into DNA in the form of nucleotide misincorporation patterns. Here, we define a theoretical framework quantifying the expected capabilities of molecular ticker tapes as a function of experimental parameters. We present a decoding algorithm for estimating time-dependent input signals, and DNAP kinetic parameters, directly from misincorporation rates as determined by sequencing. We explore the requirements for accurate signal decoding, particularly the constraints on (1) the polymerase biochemical parameters, and (2) the amplitude, temporal resolution, and duration of the time-varying input signals. Our results suggest that molecular recording devices with kinetic properties similar to natural polymerases could be used to perform experiments in which neural activity is compared across several experimental conditions, and that devices engineered by combining favorable biochemical properties from multiple known polymerases could potentially measure faster phenomena such as slow synchronization of neuronal oscillations. Sophisticated engineering of DNAPs is likely required to achieve molecular recording of neuronal activity with single-spike temporal resolution over experimentally relevant timescales.
Switching losses of IGBTs under zero-voltage and zero-current switching (2002)

Turn-off switching losses of punch-through (PT) and nonpunch-through (NPT) IGBTs under hard switching (HS) and zero-voltage switching (ZVS) are presented and evaluated at 25/spl deg/C, 75/spl deg/C and 125/spl deg/C. A comparison between PT and NPT devices based on their internal device characteristics is given for HS and ZVS. The low emitter efficiency NPT IGBTs used in this paper have smaller switching losses under HS and ZVS at 125/spl deg/C than PT devices. Zero-current switching (ZCS) techniques are also evaluated for both IGBT structures. PT IGBTs are found to have lower turn-off losses than NPT IGBTs.
Chemiluminescent detection of DNA in low- and medium-density arrays. (1998)

DNA microarray technology is revolutionizing the analysis of genetic information by enabling the high-throughput analysis of gene mutations and gene expression. This has been made possible by the development of technologies that can synthesize thousands of oligonucleotide probes in situ (1) , and high-speed robotic “printing” of cDNA probes (2) on a glass chip coupled with fluorescent labeling and detection of hybridized nucleic acids.

The technologies involved in such analyses are out of the reach of low-budget research and clinical laboratories. Here we demonstrate the use of chemiluminescent detection in a simple and inexpensive method of detecting plasmid DNA and oligonucleotide arrays on nylon membranes and glass slides.

A new method for rapid chemiluminescent detection of DNA arrays on nylon membranes and glass slides was developed using an ultrasensitive chemiluminescent reagent, LumigenTM APS-5 (Fig. 1⇓ A). The speed, sensitivity, and resolution of DNA spotted in low- and medium-density arrays was assessed with this reagent using several solid supports.

Lumigen APS-5 Chemiluminescent Substrate (Lumigen, Inc.) is supplied as an optimized formulation containing the substrate Lumigen APS-5 and lucigenin in pH 8.8 Tris buffer. DNA was spotted manually with fine-tipped pipet prepared by drawing out a Pasteur pipet over a flame. Higher density arrays were applied under a dissecting microscope. Nylon membranes from several commercial suppliers were evaluated, and polylysine-coated glass slides were from Sigma Chemical Co. The spotting was done in rows or in 1 × 1 …
High heat from a small package (1986)

Circuitry contained in a single 100-chip IBM Thermal Conduction Module (TCM) would occupy a space as large as the Empire State Building if it were built with the vacuum-tube technology of the 1950's. The amount of power needed to energize the vacuum tubes would be enormous compared to the 580 W dissipated in a TCM in today's IBM 3090 computer. One might suppose, therefore, that thermal management in computers would be relatively simpler than in the past. But with each new system that is introduced, engineers are faced with greater challenges in the cooling of microelectronic packages. A case in point is the recently announced Fujitsu system that will have 336 chips mounted on both sides of a printed circuit board of 54 x 49 cm. Each chip dissipates approximately 10 W, and the whole board is believed to dissipate more than 3 kW. Computers are becoming increasingly harder to cool, in part because microelectronic devices are becoming functionally denser. The printed features on Very Large Scale Integration (VLSI) devices are shrinking to less than a micron in size, and a minimum size of 1000 Angstroms is not far in the future. With this miniaturization, the scale of circuit integrationmore » has increased from one transistor per circuit in 1958 and fewer than 100 components (transistors, diodes, resistors, or capacitors) per chip in the early 1960's, to approximately 100,000 in 1980. In 1982, chip-gate density jumped to 450,000 components in the Hewlett-Packard 32-bit CPU chip and to 460,000 in Texas Instruments' static RAM chip. This five-orders-of-magnitude increase in circuit integration in the past 25 years is also the result of successive revolutions in device technology, from transitor/transistor logic (TTL), JTOS emitter-coupled logic (ECL), to negative metal-oxide semiconductors (NMOS), and most recently, to complementary metal-oxide semiconductors (CMOS).« less
The Synthesis of Sodium Ethylenesulfonate from Ethylene (1957)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe Synthesis of Sodium Ethylenesulfonate from EthyleneDavid S. Breslow and Robert R. HoughCite this: J. Am. Chem. Soc. 1957, 79, 18, 5000–5002Publication Date (Print):September 1, 1957Publication History Published online1 May 2002Published inissue 1 September 1957https://pubs.acs.org/doi/10.1021/ja01575a046https://doi.org/10.1021/ja01575a046research-articleACS PublicationsRequest reuse permissionsArticle Views216Altmetric-Citations16LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Retrovirus-mediated gene transfer of human pyruvate kinase (PK) cDNA into murine hematopoietic cells: implications for gene therapy of human PK deficiency (1994)

With the advent of recent molecular studies, nonspherocytic hemolytic anemia caused by red blood cell pyruvate kinase (PK) deficiency is now considered to be caused by a structural mutation of the PK-LR gene. Because PK deficiency is a monogenic disorder, the introduction of the normal PK gene into a patient's bone marrow stem cells should cure the disorder. To study the feasibility of gene therapy for PK deficiency, we first constructed the PK retrovirus pMNSM-hPK using human liver-type PK (LPK) cDNA and obtained a producer cell line of E86/AmPK. By using the supernatant of this virus-producer cell, we transduced NIH/3T3 cells, mouse leukemic cells (NFS60, FDCP-2), and human leukemic cells (K562, HEL). The expression of human LPK enzyme activity was ascertained from the retrovirally transduced NIH/3T3 cells. Northern blot analysis demonstrated the expression of the human LPK mRNA in each transduced cell line. Furthermore, bone marrow stem cells (c-kit+, Lin-, Thy-1lo) sorted by fluorescence-activated cell sorting were also transduced by the producer cells in the presence of interleukin-3 and interleukin-6, and were transplanted into lethally irradiated C57BL/6 mice. Polymerase chain reaction analysis demonstrated the expression of human LPK mRNA in both the peripheral blood and hematopoietic organs on day 30 and on day 135 of bone marrow transplantation.
High-energy electron gun with gas amplification of the filament current (1976)

A high-energy electron beam is generated using gas-ionizing collisions to amplify a small cathode filament current. The technique makes use of a crossed magnetic and electric field to reduce the effective mean free path of electrons at the cathode. At a pressure of 5×10−5 Torr helium, for example, a filament current of 0.4 mA will produce a uniform beam of 100 mA. Accelerating voltages up to 100 kV are used.
Skin Toxicity Evaluation Protocol With Panitumumab (STEPP), a Phase II, Open-Label, Randomized Trial Evaluating the Impact of a Pre-Emptive Skin Treatment Regimen on Skin Toxicities and Quality of Life in Patients With Metastatic Colorectal Cancer (2010)

The pre-emptive skin treatment regimen was well tolerated. The incidence of specific >or= grade 2 skin toxicities during the 6-week skin treatment period was reduced by more than 50% in the pre-emptive group compared with the reactive group. Patients in the pre-emptive group reported less QOL impairment than patients in the reactive group.
Lucid Data Dreaming for Multiple Object Tracking (2017)

Convolutional networks reach top quality in pixel-level object tracking but require a large amount of training data (1k ~ 10k) to deliver such results. We propose a new training strategy which achieves state-of-the-art results across three evaluation datasets while using 20x ~ 100x less annotated data than competing methods. Instead of using large training sets hoping to generalize across domains, we generate in-domain training data using the provided annotation on the first frame of each video to synthesize ("lucid dream") plausible future video frames. In-domain per-video training data allows us to train high quality appearance- and motion-based models, as well as tune the post-processing stage. This approach allows to reach competitive results even when training from only a single annotated frame, without ImageNet pre-training. Our results indicate that using a larger training set is not automatically better, and that for the tracking task a smaller training set that is closer to the target domain is more effective. This changes the mindset regarding how many training samples and general "objectness" knowledge are required for the object tracking task.
Pi-Pi contacts are an overlooked protein feature relevant to phase separation (2018)

Protein phase separation is implicated in formation of membraneless organelles, signaling puncta and the nuclear pore. Multivalent interactions of modular binding domains and their target motifs can drive phase separation. However, forces promoting the more common phase separation of intrinsically disordered regions are less understood, with suggested roles for multivalent cation-pi, pi-pi, and charge interactions and the hydrophobic effect. Known phase-separating proteins are enriched in pi-orbital containing residues and thus we analyzed pi-interactions in folded proteins. We found that pi-pi interactions involving non-aromatic groups are widespread, underestimated by force-fields used in structure calculations and correlated with solvation and lack of regular secondary structure, properties associated with disordered regions. We present a phase separation predictive algorithm based on pi interaction frequency, highlighting proteins involved in biomaterials and RNA processing.
Skill acquisition from human experts through pattern processing of teaching data (2003)

An approach to the teaching of manipulative skills is developed and applied to a deburring robot. Teaching data acquired from a human expert, who can perform an efficient job, are processed on a computer to attain his skilful manipulation strategies. The strategies are described by a group of control laws that relate sensor signals to motion commands. Sensor signals are processed by using pattern recognition techniques to interpret sensor information and to allow the robot to recognize the state of the process. The control laws designate which control action the robot should take in response to each signal pattern generated in the process. A method for driving a compact set of discrimination functions for real-time recognition of signal patterns is also discussed. The method is implemented on a direct-drive deburring robot. It is demonstrated that the robot can mimic the skilful manipulation of the human expert and perform the task efficiently.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
FCC regenerator oxygen enrichment (1995)

A direct, cost-effective method to debottleneck cracking units, which are constrained by air-blower capacity, velocity or pressure considerations in the regenerator or downstream units, or coke combustion kinetics, is to enrich the combustion air with a higher purity oxygen gas stream. Presented in this paper are: (1) a summary of the recent advances in air separations technology for the production of medium-purity oxygen, which combined with the increasing requirement for higher sulfur recovery in refineries, have considerably improved the economic benefits of using oxygen-enriched air, (2) a summary of materials combustibility studies targeted at safety considerations for refining application, especially catalytic cracking, and (3) a summary of laboratory studies which were aimed at understanding the effect of oxygen enrichment on regenerator nitrogen and sulfur chemistry.
Representations and modules (2010)

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