gebhart/patrepeval huggingface dataset:
Efficient one‐step production of astaxanthin by the microalga <i>Haematococcus pluvialis</i> in continuous culture (2005)

The performance of Haematococcus pluvialis in continuous photoautotrophic culture has been analyzed, especially from the viewpoint of astaxanthin production. To this end, chemostat cultures of Haematococcus pluvialis were carried out at constant light irradiance, 1,220 microE/m2.s, and dilution rate, 0.9/d, but varying the nitrate concentration in the feed medium reaching the reactor, from 1.7 to 20.7 mM. Both growth and biomass composition were affected by the nitrate supply. With saturating nitrate, the biomass productivity was high, 1.2 g/L.d, but astaxanthin accumulation did not take place, the C/N ratio of the biomass being 5.7. Under moderate nitrate limitation, biomass productivity was decreased, as also did biomass concentration at steady state, whereas accumulation of astaxanthin developed and the C/N ratio of the biomass increased markedly. Astaxanthin accumulation took place in cells growing and dividing actively, and its extent was enhanced in response to the limitation in nitrate availability, with a recorded maximum for astaxanthin cellular level of 0.8% of dry biomass and of 5.6 mg/L.d for astaxanthin productivity. The viability of a significant continued generation of astaxanthin-rich H. pluvialis cells becomes thus demonstrated, as also does the continuous culture option as an alternative to current procedures for the production of astaxanthin using this microalga. The intensive variable controlling the behavior of the system has been identified as the specific nitrate input, and a mathematical model developed that links growth rate with both irradiance and specific nitrate input. Moreover, a second model for astaxanthin accumulation, also as a function of irradiance and specific nitrate input, was derived. The latter model takes into account that accumulation of astaxanthin is only partially linked to growth, being besides inhibited by excess nitrate. Simulations performed fit experimental data and emphasize the contention that astaxanthin can be efficiently produced under continuous mode by adjustment of the specific nitrate input, predicting even higher values for astaxanthin productivity. The developed models represent a powerful tool for management of such an astaxanthin-generating continuous process, and could allow the development of improved systems for the production of astaxanthin-rich Haematococcus cells.
Reflective electron beam lithography: lithography results using CMOS controlled digital pattern generator chip (2013)

Maskless electron beam lithography can potentially extend semiconductor manufacturing to the 10 nm logic (16 nm half pitch) technology node and beyond. KLA-Tencor is developing Reflective Electron Beam Lithography (REBL) technology targeting high-volume 10 nm logic node performance. REBL uses a novel multi-column wafer writing system combined with an advanced stage architecture to enable the throughput and resolution required for a NGL system. Using a CMOS Digital Pattern Generator (DPG) chip with over one million microlenses, the system is capable of maskless printing of arbitrary patterns with pixel redundancy and pixel-by-pixel grayscaling at the wafer. Electrons are generated in a flood beam via a thermionic cathode at 50-100 keV and decelerated to illuminate the DPG chip. The DPG-modulated electron beam is then reaccelerated and demagnified 80-100x onto the wafer to be printed. Previously, KLA-Tencor reported on the development progress of the REBL tool for maskless lithography at and below the 10 nm logic technology node. Since that time, the REBL team has made good progress towards developing the REBL system and DPG for direct write lithography. REBL has been successful in manufacturing a CMOS controlled DPG chip with a stable charge drain coating and with all segments functioning. This DPG chip consists of an array of over one million electrostatic lenslets that can be switched on or off via CMOS voltages to pattern the flood electron beam. Testing has proven the validity of the design with regards to lenslet performance, contrast, lifetime, and pattern scrolling. This chip has been used in the REBL demonstration platform system for lithography on a moving stage in both PMMA and chemically amplified resist. Direct imaging of the aerial image has also been performed by magnifying the pattern at the wafer plane via a mag stack onto a YAG imaging screen. This paper will discuss the chip design improvements and new charge drain coating that have resulted in a functional DPG chip and will evaluate the current chip performance on the REBL system. Print results for line/space and device test patterns at the 100nm node will be presented.
Enhancing wireless information and power transfer by exploiting multi-antenna techniques (2015)

This article reviews an emerging wireless information and power transfer (WIPT) technique with an emphasis on its performance enhancement employing multi-antenna techniques. Compared to traditional wireless information transmission, WIPT faces numerous challenges. First, it is more susceptible to channel fading and path loss, resulting in a much shorter power transfer distance. Second, it gives rise to the issue of how to balance spectral efficiency for information transmission and energy efficiency for power transfer in order to obtain an optimal tradeoff. Third, there exists a security issue for information transmission in order to improve power transfer efficiency. In this context, multi-antenna techniques, e.g. energy beamforming, are introduced to solve these problems by exploiting spatial degree of freedom. This article provides a tutorial on various aspects of multi-antenna based WIPT techniques, with a focus on tackling the challenges by parameter optimization and protocol design. In particular, we investigate the WIPT tradeoffs based on two typical multi-antenna techniques: the limited feedback multi-antenna technique for short-distance transfer; and the large-scale multiple-input multiple-output (LS-MIMO, also known as massive MIMO) technique for long-distance transfer. Finally, simulation results validate the effectiveness of the proposed schemes.
Assembly of biomacromolecule loaded polyelectrolyte multilayer capsules by using water soluble sacrificial templates (2012)

In the quest for greater control over biomacromolecular loading and higher encapsulation efficiencies for biomacromolecule loaded microcapsules we devised a novel approach employing water soluble sacrificial templates. In traditional layer by layer (LbL) methods, aqueous solutions of polyelectrolyte salts in combination with water insoluble sacrificial template materials are used to prepare polyelectrolyte microcapsules that can be loaded with biomacromolecules. Here, we replaced the aqueous phase with pure aliphatic alcohols (Reversed-Phase) to greatly enhance the retention of biomacromolecular cargo close to 100% during microcapsule preparation in this Reverse-Phase Layer by Layer (RP-LbL) process. Formation of stable multilayered polyelectrolyte membranes onto water soluble template materials by sequential deposition of polystyrenesulfonic acid (PSS) and polyallylamine (PA) from pure 1-butanol is reported for the first time. The challenge to exert control over the biomacromolecule concentration within the template material and the resulting microcapsules was addressed by sacrificial template materials. Sacrificial template materials are water soluble and comprise of biomacromolecules embedded into a matrix of small molecular weight molecules such as glucose. Control over the concentration of biomacromolecules in the template material and microcapsules is conveniently exerted by adjusting weight ratios of bimacromolecules to sacrificial template material. This approach is envisioned to be applied alternatively to traditional polyelectrolyte microcapsule preparation techniques in cases where minute losses of expensive biomacromolecules are unfavorable or when accurate control over biomacromolecule concentration is important.
GP96 and the immune response (2001)

Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Biology, 2001.
Variable Hypocoagulant Effect of Fish Oil Intake in Humans (2004)

Dietary omega-3 PUFAs provoke a hypocoagulant, vitamin K-independent effect in humans, the degree of which may depend on fibrinogen level. Intake of fish oil reduced fibrinogen and factor V levels as well as thrombin generation in plasma. The effects on thrombin generation and fibrinogen clustered around subjects with high fibrinogen carrying alpha-chain fibrinogen polymorphism. Thus, dietary fish oil can provoke a hypocoagulant effect depending on the fibrinogen level.
Preclinical and clinical profile of imatinib mesilate, a potent protein-tyrosine kinase inhibitor for CML therapy (2003)

Imatinib mesilate (Glivec) is a protein-tyrosine kinase inhibitor that potently inhibits the Bcr-Abl tyrosine kinase as well as the receptors for platelet-derived growth factor (PDGF) and stem cell factor (SCF), c-Kit, at in vitro and cellular kinase assay levels. Since Bcr-Abl tyrosine kinase plays a key role in chronic myelogenous leukemia (CML) patients, treatment with imatinib mesilate that potently inhibits Bcr-Abl tyrosine kinase could be a promising therapeutic approach to CML. Imatinib mesilate was shown to inhibit proliferation of bcr-abl-positive cell lines and suppress the formation of bcr-abl-positive colonies in cells derived from bone marrow of CML patients. This compound induced apoptosis in a variety of bcr-abl-positive cells. Moreover, in vivo data indicated that imatinib mesilate suppress growth and formation of bcr-abl-positive tumors in mice. As the profile expected from the preclinical studies, imatinib mesilate showed impressive hematological and cytogenic responses in the clinical trials, including interferon-alpha-resistant or intolerant patients.
Extension of the Low Pressure Range of the Ionization Gauge (1950)

First Page
Acid−Base Equilibria in Nonpolar Media. 1. A Spectrophotometric Method for Acidity Measurements in Heptane (1997)

A UV-vis spectrophotometric method for measurements of relative acidities in heptane has been developed. The phosphazene base t-BuP(4) is used as the deprotonating agent. Its protonated form is a good counterion for the anions of the acids because it is bulky, has delocalized charge, and therefore does not have specific interactions with the anions. A self-consistent scale of relative acidities in heptane spanning for 3 pK(a) units has been constructed.
ChemInform Abstract: A Cytotoxic Lipopeptide from the Sponge‐Derived Fungus Aspergillus versicolor (2010)

Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) Suppresses TKR-Growth Factor Signaling Independent of Metalloproteinase Inhibition (2001)

The tissue inhibitors of metalloproteinases (TIMPs) block matrix metalloproteinase (MMP)-mediated increases in cell proliferation, migration, and invasion that are associated with extracellular matrix (ECM) turnover. Here we demonstrate a direct role for TIMP-2 in regulating tyrosine kinase-type growth factor receptor activation. We show that TIMP-2 suppresses the mitogenic response to tyrosine kinase-type receptor growth factors in a fashion that is independent of MMP inhibition. The TIMP-2 suppression of mitogenesis is reversed by the adenylate cyclase inhibitor SQ22536, and implicates cAMP as the second messenger in these effects. TIMP-2 neither altered the release of transforming growth factor alpha from the cell surface, nor epidermal growth factor (EGF) binding to the cognate receptor, EGFR. TIMP-2 binds to the surface of A549 cells in a specific and saturable fashion (K(d) = 147 pm), that is not competed by the synthetic MMP inhibitor BB-94 and is independent of MT-1-MMP. TIMP-2 induces a decrease in phosphorylation of EGFR and a concomitant reduction in Grb-2 association. TIMP-2 prevents SH2-protein-tyrosine phosphatase-1 (SHP-1) dissociation from immunoprecipitable EGFR complex and a selective increase in total SHP-1 activity. These studies represent a new functional paradigm for TIMP-2 in which TIMP suppresses EGF-mediated mitogenic signaling by short-circuiting EGFR activation.
Multi-modal optical microcavities for loss avoidance (2013)

We demonstrate optical microcavities wherein multiple guided modes interfere to avoid scattering loss at sidewall contacts. Cavities with 62 direct silicon contacts show resonances with intrinsic quality factors near 40,000 across an 80nm spectral range.
A highly convenient procedure for the synthesis of 5-(2-acylvinyl)uracils, a group of novel 5-substituted uracils (1990)

N. G. Kundu, L. N. Chaudhuri and S. K. Dasgupta, J. Chem. Soc., Perkin Trans. 1, 1990, 1822 DOI: 10.1039/P19900001822
Imaging distance measurements using TOF lidar (1998)

This paper presents a new time-of-flight-based imaging lidar for measurements in the 5-100 m range. The paper describes the concept of the device, shows the design of the time-to-digital converter for parallel time-interval measurements and presents the sensor breadboard. Test results showing sensor performance are also discussed. Although the device is developed for future space applications it can also be used for numerous industrial measurements.
Implication of Galectin-3 in Wnt Signaling (2005)

Galectin-3 (gal-3), a member of the beta-galactoside-binding proteins family, was identified as a binding partner of beta-catenin. Analysis of the human gal-3 sequence reveled a structural similarity to beta-catenin as it also contains the consensus sequence (S92XXXS96) for glycogen synthase kinase-3beta (GSK-3beta) phosphorylation and can serve as its substrate. In addition, Axin, a regulator protein of Wnt that complexes with beta-catenin, also binds gal-3 using the same sequence motif identified here by a deletion mutant analysis. The data presented here give credence to the suggestion that gal-3 is a key regulator in the Wnt/beta-catenin signaling pathway and highlight the functional similarities between gal-3 and beta-catenin.
Reliability of Metallized Ceramic/Polyimide Substrates (1986)

A new packaging technology has been developed to allow increased switching speeds, lower noise levels, and increased circuit density. The package consists of a ceramic substrate with two layers of copper circuitry separated by a polyimide layer. Extensive accelerated stress testing was performed on the chip carrier to determine the reliability exposures. We found no dielectric or interlayer failures after 2000 h at 85°C/80 percent relative humidity (RH). Almost no detectable resistance drift was observed in the interlayer connections. It was concluded that the polyimide chip carrier is very stable and will meet or exceed all design objectives.
Polybutenes—a New Control for Phytophagous Mites (1959)

Journal Article Polybutenes—a New Control for Phytophagous Mites Get access R. W. Fisher R. W. Fisher Entomology Labaratary, Research Branch, Canada Department of Agriculture, Vineland Station, Ontario Search for other works by this author on: Oxford Academic PubMed Google Scholar Journal of Economic Entomology, Volume 52, Issue 5, 1 October 1959, Page 1015, https://doi.org/10.1093/jee/52.5.1015 Published: 01 October 1959 Article history Accepted: 30 April 1959 Published: 01 October 1959
Optical properties of quasi-periodic (self-similar) structures (1998)

The optical properties of layered materials of a fractal quasiperiodic structure are reviewed considering both the case of wavelength smaller than the thickness of the thinner layer and the reverse case in which the whole structure is much smaller than the wavelength.
On coding for 'stuck-at' defects (Corresp.) (1987)

Additive linear codes for use on the defect channel--a model for computer memories with stuck-at defects--are studied. Basic properties of both block and convolutional codes are given. Error probabilities are carefully defined and bounded. A reasonably practical convolutional coding scheme is described and simulated. Finally, some codes for a bursty defect channel are described.
Comparison of Three Techniques for Swine Temporomandibular Joint Space Injection (2016)

The PI technique was more accurate than the LI or ASI techniques in accessing the swine superior TMJ space.
PORCINE THYMUS SUPPORTS DEVELOPMENT OF FUNCTIONAL HUMAN T CELLS (1998)

40 Since immune responses toward xenogeneic organs tend to be greater than those to allografts, unacceptably high levels of immunosuppression would likely be required for their success. A state of donor-specific tolerance would obviate the risk of both acute and chronic rejection while eliminating the need for chronic immunosuppressive therapy, thereby permitting normal immunocompetence. We now demonstrate that fetal porcine thymus grafts can reconstitute a polyclonal human T cell repertoire when co-transplanted with fetal human liver under the kidney capsule of SCID mice (SW/HU). Single positive CD4 and CD8 human T cells repopulated the peripheral lymphoid organs of the grafted SCID mice. To determine the functional status of T cells developing n SW/HU SCID thymic grafts, human T cells were stimulated with Concavalin A and phytohematoagglutinin. Human lymphocytes in porcine thymus grafts or mouse spleens showed substantial proliferative responses to lectin stimulation (stimulation indices >50). Con A-stimulated thymocytes from HU/HU and SW/HU grafts showed similar upregulation of the early activation markers CD25 and CD69 at 24 hours. In order to further assess the function of human T cells that developed in thymic xenografts, we performed allogeneic anti-human mixed lymphocyte reactions (MLR). Human lymphocytes that developed in porcine thymic grafts mounted comparable MLR alloresponses to those elicited from human lymphocytes that developed in HU/HU grafts. No evidence of graft-versus-host disease, autoimmune disease, wasting syndrome or rejection of swine grafts was detected in any SW/HU SCID mice, presumably due to tolerance of human and swine lymphocytes towards mouse, swine and human antigens. Consistent with this possibility, porcine and murine class I+ and class II+ cells with a dendritic morphology were detected by immunohistochemistry, mainly in the corticomedullary junction and the medulla of SW/HU grafts. In summary, this study provides the first evidence that the swine thymus can support normal maturation and export to the periphery of functional polyclonal human lymphocytes in vivo. In addition to inducing xenogeneic transplantation tolerance, the xenogeneic thymus grafting approach could be utilized in the treatment of patients with HIV-associated thymic atrophy, given the HIV resistance of swine tissues.
A high-precision mechanical absolute-rotation sensor (2014)

We have developed a mechanical absolute-rotation sensor capable of resolving ground rotation angle of less than 1 nrad/√Hz above 30 mHz and 0.2 nrad/√Hz above 100 mHz about a single horizontal axis. The device consists of a meter-scale beam balance, suspended by a pair of flexures, with a resonance frequency of 10.8 mHz. The center of mass is located 3 μm above the pivot, giving an excellent horizontal displacement rejection of better than 3 × 10(-5) rad/m. The angle of the beam is read out optically using a high-sensitivity autocollimator. We have also built a tiltmeter with better than 1 nrad/√Hz sensitivity above 30 mHz. Co-located measurements using the two instruments allowed us to distinguish between background rotation signal at low frequencies and intrinsic instrument noise. The rotation sensor is useful for rotational seismology and for rejecting background rotation signal from seismometers in experiments demanding high levels of seismic isolation, such as Advanced Laser Interferometer Gravitational-wave Observatory.
The Transcriptome of the Intraerythrocytic Developmental Cycle of Plasmodium falciparum (2003)

Plasmodium falciparum is the causative agent of the most burdensome form of human malaria, affecting 200-300 million individuals per year worldwide. The recently sequenced genome of P. falciparum revealed over 5,400 genes, of which 60% encode proteins of unknown function. Insights into the biochemical function and regulation of these genes will provide the foundation for future drug and vaccine development efforts toward eradication of this disease. By analyzing the complete asexual intraerythrocytic developmental cycle (IDC) transcriptome of the HB3 strain of P. falciparum, we demonstrate that at least 60% of the genome is transcriptionally active during this stage. Our data demonstrate that this parasite has evolved an extremely specialized mode of transcriptional regulation that produces a continuous cascade of gene expression, beginning with genes corresponding to general cellular processes, such as protein synthesis, and ending with Plasmodium-specific functionalities, such as genes involved in erythrocyte invasion. The data reveal that genes contiguous along the chromosomes are rarely coregulated, while transcription from the plastid genome is highly coregulated and likely polycistronic. Comparative genomic hybridization between HB3 and the reference genome strain (3D7) was used to distinguish between genes not expressed during the IDC and genes not detected because of possible sequence variations. Genomic differences between these strains were found almost exclusively in the highly antigenic subtelomeric regions of chromosomes. The simple cascade of gene regulation that directs the asexual development of P. falciparum is unprecedented in eukaryotic biology. The transcriptome of the IDC resembles a "just-in-time" manufacturing process whereby induction of any given gene occurs once per cycle and only at a time when it is required. These data provide to our knowledge the first comprehensive view of the timing of transcription throughout the intraerythrocytic development of P. falciparum and provide a resource for the identification of new chemotherapeutic and vaccine candidates.
IMAGENS DA COLONIZAÇÃO: LEITURA DE “BRASIL DE MADRUGADA” E “BANZO” (2013)

Normal 0 21 false false false MicrosoftInternetExplorer4 Estudo sobre a producao poetica de Luis da Câmara Cascudo.  A leitura dos poemas estabeleceu pontos de interesse comuns entre o poeta e outros que estavam situados historicamente em realidades consideradas perifericas do complexo cultural ocidental, cujos sistemas literarios tambem podem ser estudados do ponto de vista adotado. O artigo realiza uma leitura de poemas cuja tematica gira em torno de aspectos da colonizacao do Brasil, estabelecendo um dialogo com as vozes que construiram essa historia. Verificou-se a contribuicao do poeta ao movimento modernista brasileiro e foi demonstrada a forca da poesia como polo atrativo da percepcao de mundo do intelectual que produziu uma das mais importantes obras da cultura brasileira.
La literatura yidish de Cuba (2002)

The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.
Molecular mechanics across chemistry (1997)

1. Overview 2. Organics 3. Peptides and Proteins 4. Drug Design 5. DNA 6. Synthetic Polymers 7. Inorganics 8. Force Fields Appendices Index
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions (2003)

Our assay has important clinical applications, providing accurate diagnostic results in less time, from less biopsy material and at less cost than assays currently employed such as FISH or Southern blotting.