gebhart/patrepeval huggingface dataset:
Coproporphyrinogen oxidase. I. Purification, properties, and activation by phospholipids. (1980)

Coproporphyrinogen oxidase (EC 1.3.3.3), which converts coproporphyrinogen III into protoporphyrinogen IX, was purified about 3,200-fold from bovine liver. The most purified preparation had a specific activity of 6,920 units/mg of protein, the highest value so far reported, and was homogeneous in the polyacrylamide gel electrophoresis. The enzyme was monomeric and its molecular weight was approximately 71,600. The purified enzyme was analyzed for the amino acid composition and shown to have an abundance of aromatic amino acid residues amounting to 12% of the total residues. Spectral examination did not reveal the presence of heme and flavin. No metals were detected by atomic absorption spectroscopy either. Sulfhydryl reagents, metals, and metal chelators did not affect the enzyme activity to any significant extent. On the contrary, the purified enzyme was activated by crude phospholipid extracts from liver mitochondria and commercially available phospholipids about 2- to 5-fold. An increase in Vmax by the phospholipid extract as well as phosphatidylethanolamine accompanied a decrease in Km for coproporphyrinogen III from 48 microM to 18 to 25 microM. Synthetic nonionic detergents also exhibited an activation effect, although ionic detergents diminished the activity.
Delayed and Progressive Brain Injury in Closed-Head Trauma (1993)

The importance of delayed or secondary brain insults in the eventual outcome of closed-head trauma has been documented in experimental models. To understand this phenomenon in the clinical setting, we studied a series of head-injured patients in whom multiple cranial computed tomographic (CT) scans were obtained. Patients whose follow-up CT studies revealed new intracranial lesions or worsening, compared with admission findings, were considered to have delayed cerebral injury. One hundred forty-nine (44.5%) of 337 consecutively studied patients developed delayed brain injury. There were highly significant associations (P < 0.001) between the appearance of delayed cerebral insults and the severity of the initial brain injury, the need for cardiopulmonary resuscitation in the field, the presence of coagulopathy at admission, and subdural hematoma on the initial CT scan. In addition, delayed injury was associated (P < 0.001) with higher mortality, slowed recovery, and poorer outcome at 6 months. Delayed brain injury was not significantly associated with patient age, sex, injury mechanism, associated injury, the need for endotracheal intubation in the field, early talking, CT abnormality other than intracranial hematoma, or type of residual neurological deficits. We used multiple regression analysis to explore the relationship between severity of injury, delayed insults, and outcome. As expected, the severity of the initial brain trauma contributed significantly to neurological outcome. The presence of delayed cerebral injury makes the outcome dramatically worse for each category of initial injury severity. The relationship between initial and secondary brain injury is discussed.
A facile cesium fluoride-mediated synthesis of aromatic polyethers from bisphenols and activated aromatic dihalides (1997)

A facile method for the synthesis of high-molecular-weight aromatic polyethers was developed with the use of cesium fluoride as a base. The high-temperature solution polycondensation between bisphenols and activated aromatic dihalides with cesium fluoride in polar aprotic solvents readily afforded a series of aromatic polyethers having inherent viscosities of 0.5–1.0 dL/g under essentially neutral and milder reaction conditions, compared with the conventinal method using alkali hydroxides or alkali carbonates. © 1997 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 35: 2055–2061, 1997
Gene regulation in the white–opaque transition of<i>Candida albicans</i> (1995)

Most strains of Candida albicans switch frequently and reversibly among a number of different phenotypes distinguishable by colony morphology. Previous experiments indicated that switching involved differential gene expression. Using the white–opaque transition as a model switching system, we have cloned two opaque-specific genes, PEP1 and OP4, and one white specific gene, WH11. Differential transcription of these genes suggested that switching involves the coordinate regulation of batteries of unlinked phase-specific genes. It has been demonstrated that the frequency of integration at phase specific loci is a function of the transcriptional state of the phase-specific genes. In addition, a functional dissection of the 5′-upstream region of the WH11 gene has identified two major domains containing cis-acting regulatory sequences that are involved in phase-specific transcription. Gel retardation experiments provide evidence for white phase-specific trans-acting factors which form complexes with both domains. The regulation of the switching event is discussed. Key words: Candida albicans, phenotypic switching, white–opaque transition, phase-specific genes, integrative transformation, promoter regions, WH11 gene.
Wavelet Leaders in Multifractal Analysis (2007)

The properties of several multifractal formalisms based on wavelet coefficients are compared from both mathematical and numerical points of view. When it is based directly on wavelet coefficients, the multifractal formalism is shown to yield, at best, the increasing part of the weak scaling exponent spectrum. The formalism has to be based on new multiresolution quantities, the wavelet leaders, in order to yield the entire and correct spectrum of Hölder singularities. The properties of this new multifractal formalism and of the alternative weak scaling exponent multifractal formalism are investigated. Examples based on known synthetic multifractal processes are illustrating its numerical implementation and abilities.
Portable Nanoporous Electrical Biosensor for Ultrasensitive Detection of Troponin-T (2015)

This novel technology has promising preliminary results for rapid and sensitive detection of cTnT.
Randomized, Controlled Trial of Clonidine for Smoking Cessation in a Primary Care Setting (1989)

Clonidine hydrochloride has been reported to reduce tobacco withdrawal symptoms and facilitate smoking cessation. We enrolled 185 subjects, 92 receiving clonidine and 93 receiving placebo, in a randomized, double-blind study of clonidine for smoking cessation in a primary care setting. Clonidine had no demonstrable effect on withdrawal (8 of 11 measures favoring placebo). At 4 weeks, 17 (18%) subjects receiving clonidine had quit compared with 13 (14%) receiving placebo (chi 2 = 0.7; 90% confidence interval of benefit from clonidine, -4% to 13%). At 4 weeks, the mean number of cigarettes smoked was 17.7 for those receiving clonidine and 17.5 for those receiving placebo (t = 0.1; 90% confidence interval of benefit from clonidine, -4.1 to 3.7 cigarettes per day). These results provide little support for a beneficial effect of clonidine on tobacco withdrawal symptoms, quitting, or smoking reduction in a primary care setting.
Synthesis of Retinoic Acid by Rat Ovarian Cells That Express Cellular Retinoic Acid-Binding Protein-II1 (1999)

The induction of pseudopregnancy by the injection of eCG in rats results in the appearance of cellular retinoic acid-binding protein type II (CRABP[II]) in the granulosa cells of the ovary and the lining epithelium of the uterus within 48 h. This expression pattern is also seen in the normal mature female rat, in which CRABP(II) is expressed in the uterine epithelium during estrus (but not diestrus) and in the granulosa and luteal cells of the ovary. We have previously demonstrated that the uterine epithelial cells from the pseudopregnant rat have gained the ability to synthesize retinoic acid from retinol, in correlation with the induced expression of CRABP(II). If this is true for other sites of CRABP(II) expression, then local production of retinoic acid is intimately connected with various stages of reproduction in the female. Here we report that granulosa cells from the ovary of the eCG-treated immature rat and luteal cells from the ovary of the eCG/hCG-treated immature rat (both of which express CRABP[II]) synthesized markedly higher amounts of retinoic acid when cultured, compared to granulosa cells cultured from the ovary of the prepubertal rat treated with control vehicle. Culturing the granulosa cells from either control or eCG-treated animals had no effect on the expression of CRABP(II) cells. These data are consistent with our hypothesis that CRABP(II) expression is associated with retinoic acid synthesis and strengthen the case that local generation of retinoic acid plays an important role in reproduction.
Germline Mutations in <i>SUFU</i> Cause Gorlin Syndrome–Associated Childhood Medulloblastoma and Redefine the Risk Associated With <i>PTCH1</i> Mutations (2014)

We demonstrate convincing evidence that SUFU mutations can cause classical Gorlin syndrome. Our study redefines the risk of medulloblastoma in Gorlin syndrome, dependent on the underlying causative gene. Previous reports have found a 5% risk of medulloblastoma in Gorlin syndrome. We found a < 2% risk in PTCH1 mutation-positive individuals, with a risk up to 20× higher in SUFU mutation-positive individuals. Our data suggest childhood brain magnetic resonance imaging surveillance is justified in SUFU-related, but not PTCH1-related, Gorlin syndrome.
Surface characterization and antibacterial activity of chitosan‐grafted poly(ethylene terephthalate) prepared by plasma glow discharge (2001)

Abstract Poly(ethylene terephthalate) (PET) texture was exposed to oxygen plasma glow discharge to produce peroxides on its surfaces. These peroxides were then used as catalysts for the polymerization of acrylic acid (AA) in order to prepare a PET introduced by a carboxylic acid group (PET‐A). Chitosan and quaternized chitosan (QC) were then coupled with the carboxyl groups on the PET‐A to obtain chitosan‐grafted PET (PET‐A‐C) and QC‐grafted PET (PET‐A‐QC), respectively. These surface‐modified PETs were characterized by attenuated total reflection Fourier transform IR spectroscopy, electron spectroscopy for chemical analysis, and a contact angle goniometer. The amounts of AA, chitosan, and QC grafted on the PET surfaces as determined by the gravimetric method were about 6, 8, and 9 μg/cm 2 , respectively. The antibacterial activity of the surface‐modified PET textures was investigated using a shake flask method. After 6 h of shaking, the growth of bacteria was markedly inhibited by PET with ionically (86% in PET‐A − ‐C + ) and covalently (75% in PET‐A‐C) grafted chitosan and with covalently grafted QC (83% in PET‐A‐QC). After the laundering the inhibition of the growth of the bacteria was maintained in the range of 48–58%, showing the fastness of the chitosan‐grafted PET textures against laundering. © 2001 John Wiley & Sons, Inc. J Appl Polym Sci 81: 2769–2778, 2001
Expression of Collagen XVIII and Localization of Its Glycosaminoglycan Attachment Sites (2003)

Collagen XVIII is the only currently known collagen that carries heparan sulfate glycosaminoglycan side chains. The number and location of the glycosaminoglycan attachment sites in the core protein were determined by eukaryotic expression of full-length chick collagen XVIII and site-directed mutagenesis. Three Ser-Gly consensus sequences carrying glycosaminoglycan side chains were detected in the middle and N-terminal part of the core protein. One of the Ser-Gly consensus sequences carried a heparan sulfate side chain, and the remaining two had mixed chondroitin and heparan sulfate side chains; thus, recombinant collagen XVIII was a hybrid of heparan sulfate and chondroitin proteoglycan. In contrast, collagen XVIII from all chick tissues so far assayed have exclusively heparan sulfate side chains, indicating that the posttranslational modification of proteins expressed in vitro is not entirely identical to the processing that occurs in a living embryo. Incubating the various mutated collagen XVIIIs with retinal basement membranes showed that the heparan sulfate glycosaminoglycan side chains mediate the binding of collagen XVIII to basement membranes.
Micro Flows: Fundamentals and Simulation (2002)

Basic Concepts and Technologies * Governing Equations and Slip Models * Shear-Driven and Separated Micro Flows * Pressure-Driven Micro Flows: Slip Flow Regime * Pressure-Driven Flows: Transition and Free- Molecular Regimes * Thermal Effects in Micro Scales * Prototype Applications of Gas Micro Flows * Electrokinetically-Driven Liquid Micro Flows * Numerical Methods for Continuous Simulation * Numerical Methods for Atomistic Simulation
Oxidation of glutathione during hydroperoxide metabolism (1984)

In the present study freshly isolated rat hepatocytes treated with the glutathione reductase inhibitor BCNU (1,3-bis(2-chloroethyl)-1-nitrosourea) were used to investigate the metabolism of tert-butyl hydroperoxide and of hydrogen peroxide formed in different intracellular compartments. Glycolate, benzylamine and hexobarbital were used to stimulate H2O2 production in the peroxisomal, mitochondrial and endoplasmic reticular/cytosolic compartments, respectively. Our results support previous findings that catabolism of H2O2 formed in the mitochondrial and cytosolic compartments occurs predominantly by the glutathione peroxidase system, whereas H2O2 generated within the peroxisomes is metabolized by catalase. They further reveal that the capacity of uninhibited glutathione reductase to reduce glutathione disulfide, formed during hydroperoxide metabolism by glutathione peroxidase, is high and that a decreased NADPH/NADP+ redox level, rather than insufficient reductase activity, is responsible for the accumulation and subsequent excretion of cellular glutathione disulfide observed during hydroperoxide metabolism. Finally, our results demonstrate that H2O2 generated during cytochrome P-450-mediated drug oxidation is metabolized primarily by the glutathione peroxidase system.
A dermoscopy survey to assess who is using it and why it is or is not being used (2009)

Dermoscopy is a widely used tool for the diagnosis of pigmented skin lesions. The use of dermoscopy is increasing in popularity amongst dermatologists, making it necessary to better understand dermoscopy and to analyse why physicians use it or do not use it. The survey indicated that the majority of physicians used dermoscopy in order to reduce patient anxiety and to detect melanoma early. The main reason why dermatologists found dermoscopy to be ineffective was that they felt that it was not useful in detecting melanoma earlier than traditional methods; 35% of dermatologists surveyed believed that dermoscopy required excessive training. These results imply that current training methods need to be modified. Training is limited to large dermatology centers and is not being transferred to general centers, which would allow it to be more accessible to a larger group of dermatologists in training. There is also a need to make the learning of dermoscopy easier and to establish a universal method of teaching. Overall, there is a need for improvement in the education of dermoscopy, ranging from training to information on the basics of dermoscopy. This would include aspects such as how long the average examination takes and dermoscopy's effectiveness compared with alternate methods.
Ultraviolet B-induced Activated Protein-1 Activation Does Not Require Epidermal Growth Factor Receptor but Is Blocked by a Dominant Negative PKCλ/ι (1996)

The exposure of mammalian cells to UV irradiation leads to the activation of transcription factors such as activated protein-1 (AP-1) and NFkappaB. It is postulated that epidermal growth factor (EGF) receptor, but not protein kinase C (PKC), is the major membrane mediator in UV-induced signal transduction. Since UVB is responsible for most of the carcinogenic effects of sun exposure, we investigated the role of EGF receptors and PKC in UVB-induced AP-1 activation. Our results indicated that while the down-regulation of novel PKC (nPKC) and conventional PKC (cPKC) by pretreatment of cells with 12-O-tetradecanoyl phorbol-13-acetate cannot block UVB-induced AP-1 activity, it can block 12-O-tetradecanoyl phorbol-13-acetate-induced AP-1 activity. Further, the dominant negative mutant PKClambda/iota blocked UVB-induced AP-1 activity in all doses and time courses studied. In contrast, UVB-induced AP-1 activity from cells devoid of EGF receptor (B82) was not significantly different from that of the stable transfectants with a kinase-deficient EGF receptor (B82M721) or those with a wild-type EGF receptor (B82L) at all UVB irradiation doses and time courses studied. All of this evidence indicated that aPKC, but not EGF receptor, is involved in UVB-induced AP-1 activation.
Cross-talk analysis of time-division multiplexing of polarization-insensitive fiber-optic Michelson interferometric sensors with a 3 × 3 directional coupler (1997)

A time division multiplexing of polarization-insensitive fiber-optic Michelson interferometric sensors (TDM-PIMI's) with a 3 x 3 directional coupler is presented. The elimination of polarization-induced fading and the output intensities of the TDM-PIMI system are described and demonstrated. The output intensity of each sensor of the system can be demodulated by a passive homodyne method to increase the sensor bandwidth significantly. The sensor cross talk of the system having an optical gate with a finite extinction ratio is analyzed. The use of a laser source with an adequate coherence length to reduce the sensor cross talk is suggested. The delay-fiber cross talk of the system by Rayleigh backscattering is analyzed and demonstrated. We further suggest some methods that could possibly reduce the effect of the Rayleigh backscattered light. Finally a sophisticated design of a TDM-PIMI system with a 3 x 3 directional coupler is described.
Regulation of Chronic Relapsing Experimental Allergic Encephalomyelitis by Endogenous and Exogenous Glucocorticoids (1997)

Expression, development and resolution of the acute form of experimental allergic encephalomyelitis (EAE), typically induced in the highly susceptible Lewis rat, are closely regulated by endogenous corticosteroids. Administration of synthetic glucocorticoids also efficiently controls the manifestation of disease. The pivotal role played by the corticosteroids in modifying the induction and progression of EAE is further emphasised by a reversal of corticoid-mediated effects through adrenalectomy or treatment with the steroid receptor antagonist RU486 (mifepristone). Chronic relapsing EAE (CREAE) is characterised by acute symptoms, periods of remission and re-emergence of disease. The mechanisms governing the development of CREAE are unclear, but may require the regulatory influence of endogenous glucocorticoids. The current study has monitored circulating corticosteroids throughout the course of CREAE in the Biozzi ABH mouse and found that major fluctuations in systemic levels coincide with the relapsing-remitting phases of the disease. Furthermore, increasing circulating adrenocorticoids through administration of the steroidal compound dexamethasone markedly suppresses the occurrence of acute signs. The importance of the glucocorticoids in controlling CREAE is again highlighted by the intensification of symptoms and reduction in the survival rate of inoculated mice receiving RU486 prior to and during the acute phase of disease. The data reinforce the amelioratory actions of exogenous and naturally occurring glucocorticoids in the pathogenesis of EAE and extend earlier observations in the monophasic disease by demonstrating corticosteroid-dependent effects in a relapsing-remitting mouse model.
Development of an Assay for High-Throughput Energy Expenditure Monitoring in the Zebrafish (2013)

Energy homeostasis is maintained by balancing energy intake and expenditure. Many signals regulating energy intake are conserved between the human and teleost. However, before this work, there was no sensitive high-throughput system to monitor energy expenditure in the teleost. We exploit the nonfluorescent and fluorescent properties of resazurin and its reduced form resorufin (alamarBlue(®)) to monitor energy expenditure responses to drug application and genetic manipulation. We show that leptin, insulin, and alpha-melanocyte-stimulating hormone (α-MSH) increase energy expenditure dose dependently in the larval zebrafish. As previously established in the mouse, etomoxir, a carnitine palmitoyl transferase I inhibitor, blocks leptin-induced energy expenditure in the zebrafish. Metformin, the most commonly prescribed insulin sensitizer, increases the insulin-induced metabolic rate. Using genetic knockdown, we observed that α-MSH treatment increases the metabolic rate, as does knockdown of the melanocortin antagonist, agouti-related protein. The agouti-related protein and multiple melanocortin receptors are shown to be involved in these effects. These studies confirm that aspects of hormonal regulation of energy expenditure are conserved in the teleost, and suggest that this assay may provide a unique tool to perform in vivo screens for drugs or genes that affect the metabolic rate, including insulin or leptin sensitizers.
Small Conductance Ca<sup>2+</sup>-Activated K<sup>+</sup>Channels Modulate Synaptic Plasticity and Memory Encoding (2002)

Activity-dependent changes in neuronal excitability and synaptic strength are thought to underlie memory encoding. In hippocampal CA1 neurons, small conductance Ca2+-activated K+ (SK) channels contribute to the afterhyperpolarization, affecting neuronal excitability. In the present study, we examined the effect of apamin-sensitive SK channels on the induction of hippocampal synaptic plasticity in response to a range of stimulation frequencies. In addition, the role of apamin-sensitive SK channels on hippocampal-dependent memory encoding and retention was also tested. The results show that blocking SK channels with apamin increased the excitability of hippocampal neurons and facilitated the induction of synaptic plasticity by shifting the modification threshold to lower frequencies. This facilitation was NMDA receptor (NMDAR) dependent and appeared to be postsynaptic. Mice treated with apamin demonstrated accelerated hippocampal-dependent spatial and nonspatial memory encoding. They required fewer trials to learn the location of a hidden platform in the Morris water maze and less time to encode object memory in an object-recognition task compared with saline-treated mice. Apamin did not influence long-term retention of spatial or nonspatial memory. These data support a role for SK channels in the modulation of hippocampal synaptic plasticity and hippocampal-dependent memory encoding.
Displacement method to develop highly sensitive and selective dual chemosensor towards sulfide anion (2010)

By utilizing a displacement method, a macrocyclic compound 1, could report the presence of sulfide anion, with the detection limit of 7.0 × 10(-7) mol/L; moreover, no interference was observed from other anions, including SO(3)(2-), HSO(3)(-), SO(4)(2-), ClO(4)(-), I(-), Br(-), Cl(-), F(-), IO(3)(-), HPO(4)(2-), PO(4)(3-), C(2)O(4)(2-), S(2)O(3)(2-), CO(3)(2-), AcO(-), CN(-) and P(2)O(7)(4-), making compound 1 a new, highly sensitive and selective sulfide anion chemosensor.