gebhart/patrepeval huggingface dataset:
Complex with a Phage Display-Derived Peptide Provides Insight into the Function of Insulin-like Growth Factor I (2003)

The dramatic improvement in the NMR spectra of insulin-like growth factor I (IGF-I) in the presence of a peptide identified from a phage display library has allowed for the first time the determination of a high-resolution solution structure for much of IGF-I. The three helices of IGF-I in this complex have an arrangement similar to that seen in high-resolution crystal structures of IGF-I and insulin, although there are differences in the conformation and precise location of helix 3. A cluster of hydrophobic and basic side chains within the turn-helix motif of the peptide contact a hydrophobic patch on helices 1 and 3 of IGF-I. The importance of this patch for tight binding was verified using alanine scanning mutagenesis of the peptide in two different phage display formats. Consistent with its antagonistic activity, the peptide binds to a region implicated by mutagenesis studies to be important for association with IGF binding proteins (IGFBPs). The ability of the peptide to also inhibit signaling has important implications for the manner in which IGF-I interacts with its receptor. Interestingly, the peptide uses the same binding site as detergent and a fragment of IGFBP-5 identified in other IGF-I complexes. The ligand-induced structural variability of helix 3 in these complexes suggests that exchange between such conformations may be the source of the dynamic nature of free IGF-I and likely has functional significance for the ability of IGF-I to recognize two signaling receptors and six binding proteins with high affinity.
A 0.65-V 2.5-GHz Fractional-N Synthesizer With Two-Point 2-Mb/s GFSK Data Modulation (2009)

We present ultra-low-voltage circuit design techniques for a fractional-N RF synthesizer with two-point modulation which was realized in 90-nm CMOS using only regular V <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">T</sub> devices.; the voltage controlled oscillator, phase-frequency detector and charge pump operate from a 0.5 V supply while the divider uses a 0.65 V supply. The frequency synthesizer achieves a phase noise better than -120 dBc/Hz at 3 MHz, while consuming 6 mW. A calibration technique to equalize the gain between the two modulation ports is introduced and enables phase/frequency modulation beyond the loop bandwidth of the phase-locked loop. Measurement results for 2-Mb/s GFSK modulation are presented.
Comparative actions of adrenomedullin and nitroprusside: interactions with ANG II and norepinephrine (2001)

The role of adrenomedullin (ADM) in volume and pressure homeostasis remains undefined. Accordingly, we compared the biological responses to infusions of ADM and nitroprusside (NP; matched for reduction of arterial pressure) and assessed their effects on responses to ANG II and norepinephrine in eight conscious sheep. During matched falls in arterial pressure (8-10 mmHg, both P < 0.001) ADM and NP induced similar increases in heart rate. ADM increased cardiac output (P < 0.001), and the fall in calculated peripheral resistance was greater with ADM than NP (P = 0.013). ADM infusions raised plasma ADM levels (P < 0.001), plasma renin activity (P = 0.001), and ANG II (P < 0.001) but tended to blunt any concurrent rise in aldosterone compared with NP (P = 0.056). ADM maintained both urine flow (P < 0.001) and sodium excretion (P = 0.01) compared with falls observed with NP. ADM attenuated the vasopressor actions of exogenous ANG II (P = 0.006) but not norepinephrine. In addition, ADM antagonized the ANG II-induced rise in plasma aldosterone (P < 0.001). In conclusion, ADM induces a different spectrum of hemodynamic, renal, and endocrine actions to NP. These results clarify mechanisms by which ADM might contribute to volume and pressure homeostasis.
High throughput screening of a small molecule one-bead-one-compound combinatorial library to identify attenuators of p21 as chemotherapy sensitizers (2008)

Kidney cancer is notoriously difficult to treat when metastatic due to its resistance to conventional chemotherapy. p21 is a cyclin kinase inhibitor which, in many tumor cell lines, conveys an antiapoptotic function through its induction by the DNA damage responsive p53 pathway, such that attenuation of p21 sensitizes several disparate cancer cell lines to DNA-damaging chemotherapy. Since clinical applications with therapeutic antisense and siRNA approaches are problematic, we sought to discover other methods to inhibit p21 which are more readily translatable to the clinic. Utilizing an on-bead enzyme-linked colorimetric binding assay, we screened a diverse one-bead-one-compound combinatorial small molecule library and identified 12 candidate compounds which bind p21. Each of the 12 candidate compounds was synthesized and tested individually and three ligands were found which had the highest p21 binding affinity and yielded similar chemical structure. These three compounds caused dose-dependent cytotoxicity as well as apoptosis when exposed to two RCC cell lines. In addition, these compounds sensitized cells to apoptosis when incubated with doxorubicin such that a lower dose of doxorubicin was required in the presence of the compounds for equivalent cell killing. Interestingly, a representative of the three compounds decreased p21 levels by specific induction of ubiquitin-dependent proteosome degradation. Thus, by high throughput screening of thousands of candidate small molecules, we have identified compounds which attenuate p21, cause RCC cell apoptosis and sensitize RCC cells to DNA-damaging chemotherapy. These compounds are currently being evaluated in in vivo assays as potential novel therapeutic for RCC.
Sensory Characteristics, Shear Values and Cooking Properties of Ground Beef Patties Extended with Iron‐ and Zinc‐Fortified Soy Isolate, Concentrate or Flour (1985)

ABSTRACT Ground beef patties made from 100% beef or beef extended with 20% rehydrated soy isolate, concentrate, or flour, with or without iron and zinc fortification, were evaluated for sensory, shear and cooking properties. With the exception of patties formulated with soy isolate, soy‐added patties were rated as more tender (P <0.05) than all‐beef patties. Soy isolate imparted textural characteristics to patties that were more similar to those of all‐beef patties than to those of soy concentrate or soy flour extended patties. Patties made with soy flour had the highest cooking yields. Also, patties extended with soy flour had lower incidences of rancid flavor, but higher incidences of soy flavor compared with all other beef‐soy formulations. Iron and zinc fortification produced a higher frequency of rancid flavor when used with soy isolate and concentrate.
Determination of Oceanic Carbon Dioxide Using a Carbonate-Selective Electrode (2002)

Potentiometric properties of the PVC membrane-based electrodes prepared with molecular tweezer-type neutral carriers, 3,12-bis(TFAB)CA and deoxy-3,12-bis(TFAB)CA, and trifluoroacetyl-p-decylbenzene (TFADB) were measured in buffered electrolytes (0.1 M Tris-H2SO4, pH 8.6 and 8.0) and artificial seawater. It was observed that the deoxy-3,12-bis(TFAB)CA-based electrode provides greatly enhanced carbonate selectivity over chloride (log K(CO3(2-), Cl-)POT approximately -6) and other minor anions present in seawater. Thus, we explored the possibility of applying this new carbonate-selective electrode for direct determination of oceanic carbon dioxide. The total carbon dioxide (TCO2) level in surface Yellow Sea water was determined with the deoxy-3,12-bis(TFAB)CA-based electrode, Severinghaus-type CO2 gas sensor, and the traditional potentiometric titration methods. The results showed that the carbonate-selective electrode provides accurate oceanic TCO2 determination comparable to that obtainable with the other two methods. The analytical procedure based on a carbonate-selective electrode is clearly advantageous over other conventional methods: it does not require any sample pretreatment and extra reagents other than the standard calibration solutions, while providing the measured results directly and immediately.
A universal computer language translator (1958)

Can Univac talk to Bizmac? As more and more electronic data-processing systems are put into operation, the need for rapid and efficient information interchange becomes important. Many organizations have evolved data-processing systems using a combination of different computer types, together with a variety of input and output equipment. The use of different computers in a single organization does not necessarily represent poor planning, since the requirements of one group may dictate the use of a particular type of computer which is inadequate for the requirements of a different group.
Monitoring conceptual development with text mining technologies: CONSPECT (2010)

This paper evaluates CONSPECT, a service that analyses states in a learner’s conceptual development. It combines two technologies – Latent Semantic Analysis to analyse text and Network Analysis (NA) to provide visualisations – into a technique called Meaningful Interaction Analysis (MIA). CONSPECT was designed to help both online learners and their tutors monitor their conceptual development. This paper reports on the validation experiments undertaken to determine how well LSA matches first year medical students in clustering concepts and in annotating text. The validation used several techniques, including card sorting and Likert scales. CONSPECT produces almost ‘peer’ quality results and what remains to be tested is whether it improves with more advanced learners. One of the experiments showed an average 0.7 correlation between humans and CONSPECT.
Intrathecal Morphine???An Underused Combination for Postoperative Pain Management (1989)

Department of Anesthesia, Indian University School of Medicine, Indianapolis, IN 46223. Accepted for publication December 12, 1988.
KF19514, a Phosphodiesterase 4 and 1 Inhibitor, Inhibits PAF-lnduced Lung Inflammatory Responses by Inhaled Administration in Guinea Pigs (1997)

Phosphodiesterase (PDE) 4 inhibitors are well known for their inhibitory effect on bronchoconstriction and inflammation and may be promising anti-asthma drugs. Platelet-activating factor (PAF) has been proposed as an inflammatory mediator to be relevant to asthma. It causes bronchoconstriction, airway microvascular leakage, inflammatory cell accumulation in the lung and hyperresponsiveness. In this study, we therefore have investigated the anti-asthmatic effects of the inhaled KF19514 [5-phenyl-3'-(3-pyridyl)methyl-3H-imidazo(4,5-c)(1,8) naphthyridin-4(5H)-one], a PDE 4 and 1 inhibitor, on PAF-induced lung inflammatory responses in guinea pigs. The inhaled KF19514 (0.0001-0.01%) significantly inhibited PAF-induced eosinophil and neutrophil accumulation into the airway and hyperresponsiveness in guinea pigs. The IC50 value of KF19514 against eosinophil accumulation was 14.8 microM (0.00063%). Moreover, the effect of KF19514 on the electrical field stimulation-induced bronchial contraction was examined using the main bronchi of guinea pigs in vitro. KF19514 inhibited both cholinergic and tachykininergic contraction and, in particular, produced a potent inhibitory effect on tachykininergic contraction (IC50 = 0.49 microM). The mechanism by which KF19514 inhibited the PAF-induced hyperresponsiveness may in part be the suppression of the tachykinin release. Based on these results, it was demonstrated that the inhaled KF19514 might have a significant potential effect on the inflammatory cell accumulation and hyperresponsiveness induced by PAF.
Psychopharmacology in fragile X syndrome—Present and future (2004)

In addition to cognitive disability, fragile X syndrome (FXS) is associated with behavioral problems that are often functionally limiting. There are few controlled trials to guide treatment; however, available information does suggest that medications can be quite helpful for a number of categories of behavioral disturbance in FXS. Specifically, stimulants appear to be quite useful for management of distractibility, hyperactivity, and impulsive behavior; antidepressants help with anxiety, obsessive-compulsive behaviors and mood dysregulation; and antipsychotics can reduce aggression. These medications are supportive and help minimize dysfunctional behaviors and maximize functioning. As more is learned about the neural functions of FMRP, medications in the future will be expected to target specific synaptic mechanisms dysregulated in FXS brain and thus ameliorate the cognitive deficit with resultant behavioral improvements. This article summarizes knowledge about effectiveness and approaches to management of currently available psychopharmacology for behavior in FXS and discusses early leads to future treatments for cognition.
QUANTITATIVE MEASUREMENT OF SPLICED XBP1 mRNA AS AN INDICATOR OF ENDOPLASMIC RETICULUM STRESS (2006)

The unfolded protein response (UPR) events triggered by the accumulation of unfolded protein in endoplasmic reticulum (ER) activate the three UPR signaling pathways mediated by IRE1, ATF6 and PERK. Spliced XBP1 mRNA induced by activated IRE1 is translated to the protein, a potent transcription factor that induces BiP expression. XBP1 is also induced by activated ATF6. It is thus thought to be an important marker reflecting both IRE1 and ATF6 signaling in response to ER stress. For quantitative measurement of XBP1 gene expression, it is important to distinguish between the spliced and non-spliced form of XBP1 mRNA. We have developed a new method to detect the spliced XBP1 mRNA by means of real-time PCR and we compared the result with measurements of the expression of the ER stress inducible gene BiP. A good correlation was found between spliced XBP1 expression and BiP expression. Thus, our method may be useful for simple and quantitative evaluation of ER stress.
A match made at a toxic dump (1993)

Probiotics modulate the gut microbiota, which in turn regulate immune responses to maintain balanced immune homeostasis in the host. However, it is unclear how probiotic bacteria regulate immune responses. In this study we investigated the immunomodulatory effects of heat-killed probiotics, including <i>Lactiplantibacillus plantarum</i> KC3 (LP3), <i>Lactiplantibacillus plantarum</i> CKDB008 (LP8), and <i>Limosilactobacillus fermentum</i> SRK414 (LF4), via phagocytosis, nitric oxide (NO), and pro-inflammatory cytokine production in macrophages. We thus found that heat-killed LP8 could promote the clearance of foreign pathogens by enhancing the phagocytosis of macrophages. Treatment with heat-killed LP8 induced the production of NO and pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β. In addition, heat-killed LP8 suppressed the production of NO and cytokines in LPS-induced RAW264.7 cells, suggesting that heat-killed LP8 exerts immunomodulatory effects depending on the host condition. In sum, these results indicate that heat-killed LP8 possesses the potential for immune modulation while providing a molecular basis for the development of functional probiotics prepared from inactivated bacterial cells.
Fluorine Fluorosulfonate (1956)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFluorine FluorosulfonateFrank B. Dudley, George H. Cady, and David F. Eggers Jr.Cite this: J. Am. Chem. Soc. 1956, 78, 2, 290–292Publication Date (Print):January 1, 1956Publication History Published online1 May 2002Published inissue 1 January 1956https://pubs.acs.org/doi/10.1021/ja01583a011https://doi.org/10.1021/ja01583a011research-articleACS PublicationsRequest reuse permissionsArticle Views240Altmetric-Citations31LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Detection of Circulating Tumor Cells in Early-Stage Breast Cancer Metastasis to Axillary Lymph Nodes (2007)

The detection of CTCs offers a novel means to assess the presence of systemic disease spreading relative to SLN and ALN histopathology status.
Beyond the Desktop Metaphor: Designing Integrated Digital Work Environments (2007)

The computer's metaphorical desktop, with its onscreen windows and hierarchy of folders, is the only digital work environment most users and designers have ever known. Yet empirical studies show that the traditional desktop design does not provide sufficient support for today's real-life tasks involving collaboration, multitasking, multiple roles, and diverse technologies. In Beyond the Desktop Metaphor, leading researchers and developers consider design approaches for a post-desktop future. The contributors analyze the limitations of the desktop environment--including the built-in conflict between access and display, the difficulties in managing several tasks simultaneously, and the need to coordinate the multiple technologies and information objects (laptops, PDAs, files, URLs, email) that most people use daily--and propose novel design solutions that work toward a more integrated digital work environment. They describe systems that facilitate access to information, including Lifestreams, Haystack, Task Gallery, GroupBar, and Scalable Fabric, and they argue that the organization of work environments should reflect the social context of work. They consider the notion of activity as a conceptual tool for designing integrated systems, and point to the Kimura and Activity-Based Computing systems as examples. Beyond the Desktop Metaphor is the first systematic overview of state-of-the-art research on integrated digital work environments. It provides a glimpse of what the next generation of information technologies for everyday use may look like--and it should inspire design solutions for users' real-world needs.
Biliary Excretion of Contrast Media (1970)

Experimental data are reported concerning the biliary excretion, urinary excretion and plasma protein-binding of a new organic iodine compound, coded B 9720, versus the corresponding values of two known contrast media for cholangiography—ioglycamic acid and iodipamide. In dogs, the biliary excretion of all three products is determined exclusively by active transport processes; the maximum transport value for ioglycamic acid proved greater than the corresponding values for iodipamide and B 9720. In rats, instead, the excretion of these products into the bile is a nonsaturable process. From these experimental data it appears that there is no direct relationship between the maximum biliary excretion velocity (Tm) and the degree of affinity for plasma proteins of these compounds. A strong protein-binding, nevertheless, precludes not only the urinary but also the biliary excretion at low plasma levels, when all molecules of the contrast medium are bound to plasma proteins.
Inherent and Acquired Resistance to Paclitaxel in Hepatocellular Carcinoma: Molecular Events Involved (2013)

Hepatocellular carcinoma (HCC) is a primary malignancy of the liver and is a major cause of cancer related deaths worldwide. Only 10 to 20% of HCC can be surgically excised. Therefore, chemotherapeutic intervention and treatment is essential for achieving favorable prognosis. However, therapeutic outcome of chemotherapy is generally poor owing to inherent resistance of cancer cells to the treatment or due to development of acquired resistance. To differentiate and delineate the molecular events, we developed drug resistant Hep3B cells (DRC) by treating cells with the increasing concentration of paclitaxel. We also developed a unique single cell clone of Hep3B cells (SCC) by selecting single cell colonies and screening them for resistant phenotype. Interestingly, both DRC and SCC were resistant to paclitaxel in comparison to parental Hep3B cells. We analyzed the contributory factors that may be involved in the development of resistance. As expected, level of P-glycoprotein (P-gp) was elevated in DRC. In addition, Caveolin-1 (Cav-1), Fatty acid synthase (FASN) and Cytochrome P450 (CYP450) protein levels were elevated in DRC whereas in SCC, FASN and CYP450 levels were elevated. Downregulation of these molecules by respective siRNAs and/or by specific pharmacological inhibitors resensitized cells to paclitaxel. Interestingly, these drug resistant cells were also less sensitive to vinblastine, doxorubicin and methotrexate with the exception of cisplatin. Our results suggested that differential levels of P-gp, Cav-1 and FASN play a major role in acquired resistant phenotype whereas FASN level was associated with the presentation of inherent resistant phenotype in HCC.
A Systems Biology-Based Gene Expression Classifier of Glioblastoma Predicts Survival with Solid Tumors (2009)

Accurate prediction of survival of cancer patients is still a key open problem in clinical research. Recently, many large-scale gene expression clusterings have identified sets of genes reportedly predictive of prognosis; however, those gene sets shared few genes in common and were poorly validated using independent data. We have developed a systems biology-based approach by using either combined gene sets and the protein interaction network (Method A) or the protein network alone (Method B) to identify common prognostic genes based on microarray gene expression data of glioblastoma multiforme and compared with differential gene expression clustering (Method C). Validations of prediction performance show that the 23-prognostic gene classifier identified by Method A outperforms other gene classifiers identified by Methods B and C or previously reported for gliomas on 17 of 20 independent sample cohorts across five tumor types. We also find that among the 23 genes are 21 related to cellular proliferation and two related to response to stress/immune response. We further find that the increased expression of the 21 genes and the decreased expression of the other two genes are associated with poorer survival, which is supportive with the notion that cellular proliferation and immune response contribute to a significant portion of predictive power of prognostic classifiers. Our results demonstrate that the systems biology-based approach enables to identify common survival-associated genes.