gebhart/patrepeval huggingface dataset:
Preparation of tissues for DNA flow cytometric analysis (1980)

A method for measuring DNA in tissue cells by flow cytometry utilizing a one step combination nuclear isolation-DNA fluorochrome staining procedure is described. A variety of cells and tissues, both in vivo and in vitro, was used to illustrate the universal nature of this technique. These included murine bone marrow, liver testicle, sarcoma brain tumor, rat pancreatic islets, human peripheral blood, colon mucosa, colon cancer, sarcoma and brain tumor tissues. A special nuclear isolation medium, which contained either of the DNA fluorochromes, 4',6-diamidino-2 phenylindole-2 HCl or propidium iodide, was utilized successfully to isolate single suspensions of DNA fluorochrome stained nuclei in a rapid (5-10 min), consistent manner from a variety of tissues and cells. Multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates showed that a representative sample was being obtained.
Experimental Measurement of Axial and Transaxial Resolutions of a Slit-Slat Collimator and Comparison to Theoretical Expectations (2006)

A slit-slat collimator combines a slit parallel to the axis of rotation of a gamma camera with a set of parallel septa evenly spaced in the axial direction. Since the system geometry is similar to pinhole and parallel beam, it is expected that transaxial resolution is described by pinhole relationships and axial resolution is described by parallel-beam relationships. Axial and transaxial resolutions were investigated analytically, through Monte Carlo simulations, and experimentally. A prototype slit-slat collimator was constructed from pre-existing materials. Point source data were collected over a range of source positions, and for values of the slit width, septal spacing, and septal height that varied by factors of two. Experimental FWHM resolutions agree with the theoretical and MC results.
Coordinator's Column (2018)

No AccessSIG 14 Cultural and Linguistic DiversityCoordinator’s Column1 Jan 2018Coordinator's Column Melissa White Melissa White Google Scholar More articles by this author https://doi.org/10.1044/persp3.SIG14.28 SectionsAboutFull TextPDF ToolsAdd to favoritesDownload CitationTrack Citations ShareFacebookTwitterLinked In "Coordinator's Column." Perspectives of the ASHA Special Interest Groups, 3(14), pp. 28–29 References American Speech-Language-Hearing Association. (n.d.). Advocacy. Retrieved from https://www.asha.org/ADVOCACY/ Google Scholar Additional Resources FiguresReferencesRelatedDetails Volume 3Issue 14January 2018Pages: 28-29 Get Permissions Add to your Mendeley library History Published in issue: Jan 1, 2018 Metrics Topicsasha-topicsasha-sigsasha-article-typesleader-topicsCopyright & PermissionsCopyright © 2018 American Speech-Language-Hearing AssociationPDF downloadLoading ...
Peptide mass mapping of acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with histone deacetylase (HDACs) inhibitors (2005)

The acetylated isoforms of histone H4 from mouse lymphosarcoma cells treated with HDAC inhibitors trichostatin A (TSA) and depsipeptide (DDP) were separated by acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE), in-gel digested, and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS/MS). The acetylation pattern of histone H4 in mouse lymphosarcoma cells induced by TSA was established in which acetylation initially occurred at K16 followed by K12 and then K8 and/or K5. An identical order of acetylation was found for cells treated with DDP.
Risk Factors for Severe Respiratory Syncytial Virus Lower Respiratory Tract Infection (2011)

RSV infection is a leading cause of lower respiratory tract infection, especially in High-risk infants with a history of prematurity, bronchopulmonary dysplasia (BPD), congenital heart disease (CHD), neuromusculair impairment, immunodeficiency, and Down syndrome. Host related risk factors that have been identified to be associated with severe RSV related lower respiratory tract infection include young age below 6 months at the beginning of RSV season, multiple birth, male sex, low socioeconomic status and parental education, crowded living conditions, young siblings, maternal smoking and indoor smoke pollution, malnutrition/small for gestational age, family history of atopy or asthma, low cord serum RSV antibody titers, and living at altitude.Risk factors increasing the risk of acquisition of RSV have been identified to be birth before and/or during RSV season, day care attendance, presence of older siblings in school or day-care, and lack of breast feeding. Some of these risk factors are discussed controversially and some of them are found continuously throughout the literature.Given the high cost of RSV prophylaxis, especially for the large population of late preterm infants, algorithms and risk score systems have been published that could identify high-risk infants for treatment with palivizumab out of this gestational age group. Several models reported on an average sensitivity and specificity of 70 percent and, thus, are helpful to identify infants at high risk for severe RSV infection and need for prophylaxis with palivizumab.
Spo0A Differentially Regulates Toxin Production in Evolutionarily Diverse Strains of Clostridium difficile (2013)

Clostridium difficile is an important pathogen of humans and animals, representing a significant global healthcare problem. The last decade has seen the emergence of epidemic BI/NAP1/027 and ribotype 078 isolates, associated with the onset of more severe disease and higher rates of morbidity and mortality. However, little is known about these isolates at the molecular level, partly due to difficulties in the genetic manipulation of these strains. Here we report the development of an optimised Tn916-mediated plasmid transfer system, and the use of this system to construct and complement spo0A mutants in a number of different C. difficile strain backgrounds. Spo0A is a global regulator known to control sporulation, but may also be involved in the regulation of potential virulence factors and other phenotypes. Recent studies have failed to elucidate the role of Spo0A in toxin A and toxin B production by C. difficile, with conflicting data published to date. In this study, we aimed to clarify the role of Spo0A in production of the major toxins by C. difficile. Through the construction and complementation of spo0A mutants in two ribotype 027 isolates, we demonstrate that Spo0A acts as a negative regulator of toxin A and toxin B production in this strain background. In addition, spo0A was disrupted and subsequently complemented in strain 630Δerm and, for the first time, in a ribotype 078 isolate, JGS6133. In contrast to the ribotype 027 strains, Spo0A does not appear to regulate toxin production in strain 630Δerm. In strain JGS6133, Spo0A appears to negatively regulate toxin production during early stationary phase, but has little effect on toxin expression during late stationary phase. These data suggest that Spo0A may differentially regulate toxin production in phylogenetically distinct C. difficile strain types. In addition, Spo0A may be involved in regulating some aspects of C. difficile motility.
2.07-kV AlGaN/GaN Schottky Barrier Diodes on Silicon With High Baliga’s Figure-of-Merit (2015)

In this letter, we demonstrate high-performance AlGaN/GaN Schottky barrier diodes (SBDs) on Si substrate with a recessed-anode structure for reduced turn-on voltage VON. The impact of the surface roughness after the recessed-anode formation on device characteristics is investigated. An improved surface condition can reduce the leakage current and enhance the breakdown voltage simultaneously. A low turn-on voltage of only 0.73 V can be obtained with a 50-nm recess depth. In addition, the different lengths of Schottky extension acting like a field plate are investigated. A high reverse breakdown voltage of 2070 V and a low specific ON-resistance of 3.8 mΩ · cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> yield an excellent Baliga's figure of merit of 1127 MW/cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> , which can be attributed to the low surface roughness of only 0.6 nm and also a proper Schottky extension of 2 μm to alleviate the peak electric field intensity in the SBDs.
Preliminary report on the use of oral tacrolimus (FK506) in the treatment of complicated proximal small bowel and fistulizing Crohn's disease. (1997)

Oral tacrolimus (0.15-0.29 mg/kg/day) is well absorbed in patients with Crohn's disease with proximal small bowel involvement or fistulae and appears to be of clinical benefit as a rapidly acting "bridge" to long-term therapy with methotrexate or 6-mercaptopurine.
Calorimetric and Structural Studies of Tetrabutylammonium Bromide Ionic Clathrate Hydrates B (2013)

In the present work, characteristic properties of tetrabutylammonium bromide (TBAB) ionic clathrate hydrates structures were studied by single-crystal X-ray structure analysis. The structures of three different tetragonal TBAB ionic clathrate hydrates that were formed in our experiments were based on the same water lattice of tetragonal structure I (TS-I) differing in the ways of including bromide anions and arranging tetrabutylammonium cations. We demonstrated that (1) Br– can be included into the water lattice, replacing two water molecules, (2) the butyl group of the cation can be inserted not only in large T and P cavities but also in small D cavities of the water lattice TS-I, and (3) one of the reasons for polytypism of ionic clathrate hydrates on the basis of TS-I is the occurrence of alternative modes of arrangements of four-compartment cavities in adjacent layers of the water framework. The compositions of three TBAB ionic clathrate hydrates TBAB·38.1H₂O, TBAB·32.5H₂O, and TBAB·26.4H₂O were determined by chemical analysis, and their enthalpies of fusion were measured by differential scanning calorimetry (DSC). From the obtained results, the enthalpies of the TBAB hydrate formation from TBAB and water were calculated thermodynamically.
AN ADAPTATION OF THE CASSON EQUATION FOR THE RHEOLOGY OF CHOCOLATE (1991)

ABSTRACT The generalized Casson equation with exponent m was considered. The plastic viscosities and yield values corresponding to different ranges of shear rate D were calculated for different values of exponent m between 0.5 and 1. All the plastic viscosities tend to about the same value at high shear rates, while at low shear rates all the yield values tend to the same value which is supposed to be the “true” yield value. For the exponent m = 0.6, the yield value is least dependent on the shear rate range and the yield value calculated for D > 5 sec −1 is generally much closer to the “true” yield value than the Casson value (m = 0.5). Therefore, choice of the exponent m = 0.6 is proposed.
Expression of heme oxygenase-1 by endothelial cells: a protective response to injury in transplantation (2000)

Endothelial cells (EC) play a pivotal role in the regulation of inflammation by expressing a series of pro- and anti-inflammatory genes that are associated with the activation of these cells. The nature of these genes and the regulation of their expression may be particularly important for the outcome of immediately vascularised transplants. We refer to the set of anti-inflammatory genes that are expressed during EC activation as protective genes because they can block the expression of pro-inflammatory genes associated with EC activation and prevent EC apoptosis. In this review we discuss data that supports the hypothesis that expression of these protective genes in a transplanted organ can promote its survival. We will focus on the description of one such protective gene, heme oxygenase-1 (HO-1). The first part of the review discusses the potential role of EC activation in regulating inflammatory responses such as those associated with the rejection of transplanted organs. The second part discusses the molecular mechanisms that regulate the expression of HO-1 in EC as well as the molecular mechanism by which the expression of this gene can regulate EC activation. The third part discusses potential mechanisms by which HO-1 may contribute to suppress different phases of the rejection of transplanted organs, e.g., ischaemia reperfusion injury, acute rejection and chronic failure. In the last part we discuss the role of HO-1 in establishing long-term survival of organs that are transplanted across different species, an approach referred to as xenotransplantation.
Tuning Response Curves for Synthetic Biology (2013)

Synthetic biology may be viewed as an effort to establish, formalize, and develop an engineering discipline in the context of biological systems. The ability to tune the properties of individual components is central to the process of system design in all fields of engineering, and synthetic biology is no exception. A large and growing number of approaches have been developed for tuning the responses of cellular systems, and here we address specifically the issue of tuning the rate of response of a system: given a system where an input affects the rate of change of an output, how can the shape of the response curve be altered experimentally? This affects a system's dynamics as well as its steady-state properties, both of which are critical in the design of systems in synthetic biology, particularly those with multiple components. We begin by reviewing a mathematical formulation that captures a broad class of biological response curves and use this to define a standard set of varieties of tuning: vertical shifting, horizontal scaling, and the like. We then survey the experimental literature, classifying the results into our defined categories, and organizing them by regulatory level: transcriptional, post-transcriptional, and post-translational.
A Low-Cost Construction Material (1960)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTA Low-Cost Construction MaterialAlan MichaelsCite this: Ind. Eng. Chem. 1960, 52, 9, 785–788Publication Date (Print):September 1, 1960Publication History Published online1 May 2002Published inissue 1 September 1960https://pubs.acs.org/doi/10.1021/ie50609a031https://doi.org/10.1021/ie50609a031research-articleACS PublicationsRequest reuse permissionsArticle Views43Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
Nonlinear feature based classification of speech under stress (2001)

Studies have shown that variability introduced by stress or emotion can severely reduce speech recognition accuracy.Techniques for detecting or assessing the presence of stress could help improve the robustness of speech recognition systems.Although some acoustic variables derived from linear speech production theory have been investigated as indicators of stress, they are not always consistent.In this paper, three new features derived from the nonlinear Teager energy operator (TEO) are investigated for stress classification.It is believed that the TEO based features are better able to reflect the nonlinear airflow structure of speech production under adverse stressful conditions.The features proposed include TEO-decomposed FM variation (TEO-FM-Var), normalized TEO autocorrelation envelope area (TEO-Auto-Env), and critical band based TEO autocorrelation envelope area (TEO-CB-Auto-Env).The proposed features are evaluated for the task of stress classification using simulated and actual stressed speech and it is shown that the TEO-CB-Auto-Env feature outperforms traditional pitch and mel-frequency cepstrum coefficients (MFCC) substantially.Performance for TEO based features are maintained in both text-dependent and text-independent models, while performance of traditional features degrades in text-independent models.Overall neutral versus stress classification rates are also shown to be more consistent across different stress styles.
Morphology, ultramorphology and histology of ovaries of workers and mated queens of Pachycondyla striata ants (Hymenoptera : Ponerinae) (1999)

The aim of the present work is to perform morphological and histological studies of the ovaries of workers and mated queens of Pachycondyla striata ants, which belong to the subfamily Ponerinae. The ovaries, after being removed, were schematized. Next, historesin and electronic scanning microscopy techniques were applied, making it possible to note that the left ovary owns a greater number of ovarioles when compared to the right one (workers - 7 to the right and 8 to the left; queens - 6 to the right and 7 to the left) and that the ovarioles of workers present a rather wrinkled aspect due to the fact that they are not developed. The same situation does not occur in mated queens once they always present oocytes in distinct development phases in their ovarioles. Histologically it was observed that the ovarioles are of the meroistic polytrophic kind. Inside the ovarioles of workers, germinative cells were observed in their distal portion, but their lumen were empty. on the other hand, ovarioles of mated queens presented the germinative cells as well as oocytes in different degrees of development, although more than one developing oocyte was never observed in the interior of each ovariole. It was possible to note the presence of follicular epithelium, chorion and vitellin membrane in oocytes of mated queens, which change morphologically according the oocyte development stage.
IL-4 regulates differentiation and proliferation of human precursor B cells. (1988)

The mechanism by which precursor and pre-B cells undergo differentiation is unclear; however, it is known that growth factors play an important role in this maturation process. The lymphokine, IL-4 has been shown to increase expression of class II Ag on B cells and induce B cell proliferation. In the murine system, IL-4 induced differentiation of precursor B cells into pre-B cells. In order to analyze growth factors on B cell development we have established an in vitro culture system for human bone marrow cells. We found that in the presence of IL-4, normal human precursor and pre-B cells can be induced to differentiate in the absence of cell proliferation with four days of culture. Furthermore, IL-4 depressed proliferation induced by supernatant from a T cell line. The differentiation was measured by an increase in both the number of cytoplasmic mu and surface IgM-positive cells. The effect of IL-4 on precursor and pre-B cell differentiation was detected as soon as 14 h of exposure to the lymphokine in the absence of an adherent feeder layer. These data suggest that IL-4 directly affects the differentiation process of normal human precursor and pre-B cells, and may antagonistically affect cell proliferation.
Cracking and hydrogenation of low-temperature coal tars and alkyl phenols (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCracking and hydrogenation of low-temperature coal tars and alkyl phenolsMulpuri JanardanaraoCite this: Ind. Eng. Chem. Prod. Res. Dev. 1982, 21, 3, 375–390Publication Date (Print):September 1, 1982Publication History Published online1 May 2002Published inissue 1 September 1982https://pubs.acs.org/doi/10.1021/i300007a007https://doi.org/10.1021/i300007a007research-articleACS PublicationsRequest reuse permissionsArticle Views273Altmetric-Citations12LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options Get e-Alerts
THE ISOMERIZATION OF HUMULONE (1967)

Certain aspects of the degradation of isohumulone have been studied utilizing the techniques of gas-liquid chromatography, mass spectrometry and ozonolysis. The modes of degradation and techniques employed are discussed.
Synthetic furocoumarins. 10. Synthesis of .alpha.-methylbenzo[b]furano compounds (1980)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthetic furocoumarins. 10. Synthesis of .alpha.-methylbenzo[b]furano compoundsKurt D. Kaufman and Larry E. HewittCite this: J. Org. Chem. 1980, 45, 4, 738–740Publication Date (Print):February 1, 1980Publication History Published online1 May 2002Published inissue 1 February 1980https://pubs.acs.org/doi/10.1021/jo01292a045https://doi.org/10.1021/jo01292a045research-articleACS PublicationsRequest reuse permissionsArticle Views149Altmetric-Citations7LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts