gebhart/patrepeval huggingface dataset:
Hox-2.3 upstream sequences mediate lacZ expression in intermediate mesoderm derivatives of transgenic mice (1990)

The mouse Hox-2.3 gene contains an Antp-like homeobox sequence and is expressed in a spatially restricted anteroposterior domain during development. To study the molecular basis of this differential gene regulation, we set out to characterize the cis-regulatory elements mediating Hox-2.3 expression during embryogenesis. We show that a fragment extending 1316 base pairs (bp) upstream of the transcription start site, thus corresponding to the Hox-2.4/Hox-2.3 intergenic sequences is capable of mediating luciferase gene transcription in transfected cells in vitro and lacZ expression in transgenic mice. The beta-galactosidase-staining pattern in embryos was found to be strikingly similar to the Hox-2.3 in situ hybridization pattern in intermediate mesoderm derivatives: high levels of both Hox-2.3 transcripts and beta-galactosidase activity were found in the mesonephric duct-derived epithelium of the meso- and metanephric kidney and associated ducts, from the time these structures first appeared on throughout development. The transgene apparently lacks sequences needed for correct Hox-2.3 expression in somitic and lateral plate mesoderm and in neurectoderm. These results document the involvement of distinct regulatory elements in Hox gene expression in subsets of cells with distinct developmental fate, situated at similar positions along the anteroposterior axis of the embryo.
Exposure to strong static magnetic field slows the growth of human cancer cells in vitro (1996)

Proposals to enhance the amount of radiation dose delivered to small tumors with radioimmunotherapy by constraining emitted electrons with very strong homogeneous static magnetic fields has renewed interest in the cellular effects of prolonged exposures to such fields. Past investigations have not studied the effects on tumor cell growth of lengthy exposures to very high magnetic fields. Three malignant human cell lines, HTB 63 (melanoma), HTB 77 IP3 (ovarian carcinoma), and CCL 86 (lymphoma: Raji cells), were exposed to a 7 Tesla uniform static magnetic field for 64 hours. Following exposure, the number of viable cells in each group was determined. In addition, multicycle flow cytometry was performed on all cell lines, and pulsed-field electrophoresis was performed solely on Raji cells to investigate changes in cell cycle patterns and the possibility of DNA fragmentation induced by the magnetic field. A 64 h exposure to the magnetic field produced a reduction in viable cell number in each of the three cell lines. Reductions of 19.04 +/- 7.32%, 22.06 +/- 6.19%, and 40.68 +/- 8.31% were measured for the melanoma, ovarian carcinoma, and lymphoma cell lines, respectively, vs. control groups not exposed to the magnetic field. Multicycle flow cytometry revealed that the cell cycle was largely unaltered. Pulsed-field electrophoresis analysis revealed no increase in DNA breaks related to magnetic field exposure. In conclusion, prolonged exposure to a very strong magnetic field appeared to inhibit the growth of three human tumor cell lines in vitro. The mechanism underlying this effect has not, as yet, been identified, although alteration of cell growth cycle and gross fragmentation of DNA have been excluded as possible contributory factors. Future investigations of this phenomenon may have a significant impact on the future understanding and treatment of cancer.
Microbubble dynamics around laser heated microparticles (2003)

Selective tissue damage on the cellular level can be achieved due to microbubble formation around laser heated intracellular pigments. To get an estimate on damage ranges we investigated microbubble dynamics (lifetime, size) around isolated melanosomes of the retinal pigment epithelium in aqueous suspensions by fast flash photography and probe laser transmission. For short pulsed laser irradiation (12ns@532nm) an increasing radiant exposure results in larger bubble sizes. Bubble diameters ranging from 1μm to 10μm were observed. Applying longer pulses (1.8μs@527nm), increasing radiant exposure leads to an earlier onset of bubble growth relative to the pulse. In this case, the diameter of the bubble is nearly independent of the radiant exposure, whereas the number of bubbles during the pulse increases towards higher radiant exposures.
The C-Terminal TDP-43 Fragments Have a High Aggregation Propensity and Harm Neurons by a Dominant-Negative Mechanism (2010)

TAR DNA binding protein 43 KD (TDP-43) is an essential gene that regulates gene transcription, mRNA splicing and stability. In amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), two fatal neurodegenerative diseases, TDP-43 is fragmented, generating multiple fragments that include the C-terminal fragment of ∼25 KD. The role of these fragments in the pathogenesis of ALS and FTD is not clear. Here we investigated the aggregation propensity in various polypeptide regions of TDP-43 in mammalian cells and the effect of these fragments on cultured neurons. By expressing the full length and various TDP-43 fragments in motor neuron-derived NSC-34 cells and primary neurons, we found that both N- and C-terminal fragments of TDP-43 are prone to aggregate and the C-terminal end of RRM2 region is required, though not sufficient, for aggregation. The aggregation of the TDP-43 fragments can drive co-aggregation with the full-length TDP-43, consequently reducing the nuclear TDP-43. In addition, the TDP-43 fragments can impair neurite growth during neuronal differentiation. Importantly, overexpression of the full-length TDP-43 rescues the neurite growth phenotype whereas knockdown of the endogenous TDP-43 reproduces this phenotype. These results suggest that TDP-43 fragments, particularly the pathologically relevant C-terminal fragments, can impair neuronal differentiation by dominant-negatively interfering with the function of the full length TDP-43, thus playing a role in pathogenesis in ALS and FTD.
Influence of reductants upon optical characteristics of the DNA–Cu<sup>2+</sup> complex (1967)

Abstract The addition of reducing agents, i.e., ascorbic acid or sodium borohydride, to a DNA solution containing Cu 2+ ions causes changes in the DNA absorption spectra which are due to a new absorption band with a maximum at 280 mμ assigned to a DNA base–Cu 1+ complex. The stoichiometry of the complex is one Cu 1+ ion per four bases of DNA. The DNA–Cu 1+ complex has an increased melting temperature and rather different circular dichroism curve as compared with DNA itself. It is inferred that the above effects are caused by proton transfer along the hydrogen bond from guanine to cytosine under complexing of Cu 1+ ions with the N 7 atom of the guanine of DNA.
Development of a magnetically supported intelligent hand for automated precision assembly (1990)

Abstract The magnetically supported intelligent hand (MSIH) is a unique robot end-effector for automating precise insertion operations. This paper illustrates its structure, control system, and capability. It also presents the experimental system and the experimental results of automated insertion by the MSIH, The MSIH has a friction-free and backlash-free structure with precision 5-DOF (degrees of freedom) control due to the magnetically contactless support. The levitated unit, which holds a work-piece, is supported by the attractive magnetic forces of electromagnets. The attractive forces are controlled based on the outputs of gap sensors measuring the 5 DOF of the levitated unit. The structure allows the MSIH to have three basic functions: (1) programmable compliance; (2) precision 5-DOF actuation; and (3) 5-axis force sensing. By using these functions, the MSIH can achieve precision insertion. It can work as a programmable remote centre compliance (RCC) and can overcome the limitations of such mechanical elastic devices as the RCC. The experimental system has been developed to demonstrate the functions and capability of the MSIH for assembly automation. The MSIH has succeeded in automating precision insertion with clearance of 8
Anne Connor and John E. Tibbett, Social Workers and Health Care in Hospitals, Her Majesty's Stationery Office, London, 1988, 97 pp., £6.00, ISBN 0 114 49345 4. (1989)

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Fast mode selection algorithm based on texture analysis for 3D-HEVC intra prediction (2015)

The increasing availability of 3D video systems and applications has attracted more consumers for 3D viewing experiences and, consequently, the demand for storage and transmission of 3D video content is growing. An interesting alternative for this need is the transmission of 3D video based on the Multiview Video plus Depth (MVD) format. The upcoming 3D High Efficiency Video Coding (3D-HEVC) standard will adopt this format, which associates a depth map to each texture frame. This paper presents a fast mode decision algorithm, which analyses the texture frames and depth maps to detect the edge orientation of the prediction units (PUs), optimizing the intra prediction process and reducing the 3D-HEVC computational complexity. The experimental results show that the proposed algorithm achieves an average processing time reduction of 26.2%, with a small degradation in encoding efficiency (BD-rate increase of 0.3% on average).
Volume dependence of the elastic equation of state. 3. Bulk-cured poly(dimethylsiloxane) (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTVolume dependence of the elastic equation of state. 3. Bulk-cured poly(dimethylsiloxane)R. W. Brotzman and B. E. EichingerCite this: Macromolecules 1982, 15, 2, 531–535Publication Date (Print):March 1, 1982Publication History Published online1 May 2002Published inissue 1 March 1982https://pubs.acs.org/doi/10.1021/ma00230a061https://doi.org/10.1021/ma00230a061research-articleACS PublicationsRequest reuse permissionsArticle Views117Altmetric-Citations50LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Evaluation of the Antifungal Activity of Natural Xanthones from <i>Garcinia mangostana</i> and Their Synthetic Derivatives (1997)

The antifungal activity of several xanthones isolated from the fruit hulls of Garcinia mangostana and some derivatives of mangostin against three phytopathogenic fungi, Fusarium oxysporum vasinfectum, Alternaria tenuis, and Dreschlera oryzae, has been evaluated. The natural xanthones showed good inhibitory activity against the three fungi. Substitution in the A and C rings has been shown to modify the bioactivities of the compounds.
Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools (2018)

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.
Safety and Efficacy of Fedratinib in Patients With Primary or Secondary Myelofibrosis (2015)

clinicaltrials.gov identifier: NCT01437787.
Studies on Human Hemoglobin Treated with Various Sulfhydryl Reagents (1966)

Abstract The reactive sulfhydryl groups of human oxyhemoglobin (at position 93 of the β chains) form compounds with cystine, cystamine, dimercaptoethanol, and iodoacetamide. No reaction occurs with dithioglycolic acid, dithiobutyric acid, or diformylcystine. Some reaction occurs with iodoacetic acid but only under special conditions. The general properties (stability, spectra, molecular weight) of the modified hemoglobins described in this paper (except perhaps for the hemoglobin treated with iodoacetic acid) are very similar to those of unreacted hemoglobin. The oxygen equilibrium of these compounds has been studied. In all cases, the value of n is similar to that of normal hemoglobin (namely about 2.9) and is approximately invariant with pH. The oxygen Bohr effect is substantially reduced, and the pattern of all the results is similar in spite of the different chemical structures of the blocking reagents. The hemoglobin treated with cystine shows differential binding of the HPO4=ion as between the oxy and deoxy forms at concentrations of HPO4= in the neighborhood of 0.3 m. This explains the discrepancy between the directly measured Bohr effect for this compound and for hemoglobin treated with cystamine and that calculated from differential titrations. On the other hand, in the case of hemoglobin treated with iodoacetamide, where the newly introduced group carries no charge, there is good agreement between the Bohr effect measured by the two methods. The kinetics of combination of deoxyhemoglobins with carbon monoxide and of the dissociation of O2 from oxyhemoglobins has been studied. The kinetic behavior of all the compounds described in the present work is qualitatively similar to that of normal hemoglobin, but different from that of hemoglobin treated with p-mercuribenzoate.
A Problem-Posing Pedagogy: ‘Paths of Pleasantness and Peace’ (1999)

SCHOOLING REMAINS DOMINATED by the modernist paradigm, in which theoretical understanding is elevated above other ways of knowing, not only in respect to content but also to methodology. A Biblical wisdom perspective suggests an alternative approach, in which concrete experience is the context of learning and active engagement by learners in situations of puzzlement its motor. This hypothesis is tested in the context of philosophical and psychological explorations of related options. These contribute important insights, but are considered inadequate to the extent that they remain within the theory-practice polarity. When a variety of ways of knowing is acknowledged, the gifts of individuals can be nurtured in a cooperative environment.
Experimental test of an alignment-sensing scheme for a gravitational-wave interferometer (1998)

An alignment-sensing scheme for all significant angular degrees of freedom of a power-recycled Michelson interferometer with Fabry-Perot cavities in the arms was tested on a tabletop interferometer. The response to misalignment of all degrees of freedom was measured at each sensor, and good agreement was found between measured and theoretical values.
Endofungal bacterium controls its host by an <i>hrp</i> type III secretion system (2010)

Burkholderia rhizoxinica and Rhizopus microsporus form a unique symbiosis in which intracellular bacteria produce the virulence factor of the phytopathogenic fungus. Notably, the host strictly requires endobacteria to sporulate. In this study, we show that the endofungal bacteria possess a type III secretion system (T3SS), which has a crucial role in the maintenance of the alliance. Mutants defective in type III secretion show reduced intracellular survival and fail to elicit sporulation of the host. Furthermore, genes coding for T3SS components are upregulated during cocultivation of the bacterial symbiont with their host. This is the first report on a T3SS involved in bacterial-fungal symbiosis. Phylogenetic analysis revealed that the T3SS represents a prototype of a clade of yet uncharacterized T3SSs within the hrp superfamily of T3SSs from plant pathogenic microorganisms. In a control experiment, we demonstrate that under laboratory conditions, rhizoxin production was not required for establishment of the symbiotic interaction.
Nonlinearity and Asymmetry in the Human Vestibulo-ocular Reflex (1989)

The horizontal vestibulo-ocular reflex was studied in normal and unilaterally vestibulopathic human subjects; the latter before and after surgical vestibular ablation. Subjects were oscillated at 0.025-4 Hz, 50 o/s peak head velocity, and for 0.025 and 0.25 Hz, at 50-300 o/s peak head velocity. Gain (peak eye/head velocities), phase (delay in degrees between peak eye and head velocities), and asymmetry (including d.c. bias; the average eye velocity over the cycle) were assessed for each rotation. In patients preoperatively, gain was subnormal while phase lead was greater than normal only at lowest frequencies. As head velocity increased at 0.025 and 0.25 Hz, gain dropped in patients, but not in normals. Furthermore, d.c. bias at 0.25 Hz tended to shift abnormally toward the lesioned side in most patients. After surgery, gain dropped and phase lead rose for all stimuli, but showed varied recovery over 4 months. d.c. bias also rose after surgery, and showed an enhancement with increasing head velocity at 0.25 Hz. d.c. bias remained unchanged even after 4 months for 300 o/s rotations, but disappeared at low head velocity. d.c. bias (and other asymmetry measures) represents a lateralizing sign which can be improved by utilizing high head velocities at moderate frequencies.
Effect of Amino Acids and Oxygen on Chemotaxis in <i>Escherichia coli</i> (1966)

Adler, Julius (University of Wisconsin, Madison). Effect of amino acids and oxygen on chemotaxis in Escherichia coli. J. Bacteriol. 92:121-129. 1966.-Motile cells of Escherichia coli placed at one end of a capillary tube containing a mixture of the 20 amino acids commonly found in proteins migrate out into the tube in two bands. The bands are clearly visible to the naked eye, and they can also be demonstrated by microscopy, photography, and densitometry, and by assaying for bacteria throughout the tube. The occurrence of more than one band is not due to heterogeneity among the bacteria, since each band can be used over to give rise to two again. The first band uses all the oxygen to oxidize portions of one or more of the amino acids, including serine, and the second band consumes the residual serine anaerobically. The results are interpreted to mean that E. coli shows chemotaxis toward oxygen and serine. When no energy source is added to the medium, a band of bacteria still appears. It consumes all the oxygen to oxidize an endogenous energy source. The addition of any one of 10 oxidizable amino acids stimulates the rate of travel of this band. Alanine, an example that was studied in detail, supports such a band that consumes all the oxygen to oxidize a portion of the alanine. Serine, the only amino acid that this strain can use either aerobically or anaerobically when grown under the conditions used here, gives rise to two bands.
Power consumption in telecommunication networks: overview and reduction strategies (2011)

One of the main challenges for the future of information and communication technologies is reduction of the power consumption in telecommunication networks. The key consumers are the home gateways at the customer premises for fixed line access technologies and the base stations for wireless access technologies. However, with increasing bit rates, the share of the core networks could become significant as well. In this article we characterize the power consumption in the different types of networks and discuss strategies to reduce the power consumption.
Development of c-MET pathway inhibitors (2011)

The c-MET pathway inhibitors currently being evaluated in the clinic have demonstrated compelling evidence of clinical activity in different cancer types and may provide significant therapeutic opportunities. The challenges, however, are to identify the tumor types and patient populations that benefit most, and find the most effective combinations of therapies while minimizing potential toxicity.
Measurements of heart motion using accelerometers (2006)

We have used accelerometers to measure the heart's motion. A 3-axis sensor prototype was made from two commercially available accelerometers, and tested on anesthetized pigs. The chest wall of the pig was opened, the sensor sutured to the heart, and motion data was recorded over several hours. The heart's own beating is partly concealed by other sources of motion, particularly the respiration. High-pass filtering was used to isolate the heart beating from these other motions. The filter cut-off frequency was set to 1.0 Hz, including the heart rate at 1.5 Hz, while suppressing the respiration at 0.3 Hz. Velocity and position were obtained by numerically integrating the filtered acceleration traces. Using this procedure, the heart motion could be measured in great detail. Motion abnormalities, e.g. arrhythmias and fibrillation, were identified in the motion curves, and confirmed by comparison with synchronously recorded ECG data.
Metaproteomic evidence of changes in protein expression following a change in electrode potential in a robust biocathode microbiome (2015)

Microorganisms that respire electrodes may be exploited for biotechnology applications if key pathways for extracellular electron transfer can be identified and manipulated through bioengineering. To determine whether expression of proposed Biocathode-MCL extracellular electron transfer proteins are changed by modulating electrode potential without disrupting the relative distribution of microbial constituents, metaproteomic and 16S rRNA gene expression analyses were performed after switching from an optimal to suboptimal potential based on an expected decrease in electrode respiration. Five hundred and seventy-nine unique proteins were identified across both potentials, the majority of which were assigned to three previously defined Biocathode-MCL metagenomic clusters: a Marinobacter sp., a member of the family Chromatiaceae, and a Labrenzia sp (abbreviated as MCL). Statistical analysis of spectral counts using the Fisher's exact test identified 16 proteins associated with the optimal potential, five of which are predicted electron transfer proteins. The majority of proteins associated with the suboptimal potential were involved in protein turnover/synthesis, motility, and membrane transport. Unipept and 16S rRNA gene expression analyses indicated that the taxonomic profile of the microbiome did not change after 52 h at the suboptimal potential. These findings show that protein expression is sensitive to the electrode potential without inducing shifts in community composition, a feature that may be exploited for engineering Biocathode-MCL. All MS data have been deposited in the ProteomeXchange with identifier PXD001590 (http://proteomecentral.proteomexchange.org/dataset/PXD001590).