gebhart/patrepeval huggingface dataset:
Biochemical studies of mammalian oogenesis: Protein synthesis during oocyte growth and meiotic maturation in the mouse (1977)

ABSTRACT Using oocytes isolated from juvenile and adult mice, we have examined the qualitative patterns of protein synthesis during growth and during meiotic maturation of these oocytes. Oocytes were cultured in a defined medium in the presence of [35S]methionine and radioactively labelled proteins were separated by SDS-polyacrylamide gel electrophoresis and detected by fluorography. The results of these studies demonstrate that: (i) the patterns of protein synthesis are very similar in individual oocytes which are at the same stage of growth or of meiotic maturation, indicating a high degree of biochemical homogeneity in a given population of isolated mouse oocytes, (ii) the linear increase in protein content of growing mouse oocytes (with respect to oocyte volume) is accompanied by significant qualitative changes in the size classes of proteins synthesized, and (iii) meiotic maturation (germinal vesicle dissolution and nuclear progression to the second metaphase) is characterized by several discrete qualitative changes in the pattern of protein synthesis in the oocyte, especially during the period following germinal vesicle breakdown. Experiments carried out with oocytes cultured in the presence of drugs which have been shown to inhibit meiotic maturation at specific stages of nuclear progression suggest that: (i) protein synthesis is not required for germinal vesicle breakdown to take place; (ii) mixing of the oocyte’s nucleoplasm and cytoplasm must occur in order for those changes in the pattern of protein synthesis which characterize meiotic maturation to take place; and (iii) failure of nuclear progression to proceed beyond the circular bivalent stage does not prevent those changes in the pattern of protein synthesis which characterize meiotic maturation from taking place. The latter observations suggest that there are basic differences in the control of meiotic maturation in oocytes isolated from mammalian, as compared to non-mammalian, animal species.
Fauna de Coleoptera Lamellicornia de la región de Tépic, Nayarit, México. (1998)

Se realizo un estudio comparativo de la fauna do coleopteros Melolonthidae, Scarabaeidae, Trogidae y Passalidae en la zoria coniprendida entre Tepic, Conipostela y San José Mojarras, Nayant, México, Los datos se obtuvieron entre 1994 y 1996 principalmente con trampas de luz instaladas en los campos cultivados con caña de azúcar rodeados por remanentes de bosque tropical caducifolio y en lugares establecidos entre los 850 y 1,200 m de altitud. Se registran 11 subfamilias, 22 tribus, 52 generos y 122 especies. La mayor diversidad corresponde a Melolonthidae (36 spp.), Dynastinae (20 spp.), Scarabaeinae (17 spp.), Aphodiinae (17 spp.) y Rutelinae (14 spp.). El 50% do las especies se encuentra en los géneros phyllophaga ( 23 spp.) Diplotaxis (11 spp.), Anomala (9 spp.), Cyclocephala c) spp) y Ataenius (10spp,) Las especies más abundantes son; Phyllophaga dentex, Ph. ravida, Ph Ienis, Ph lalanza, Ph brebidens, Ph. obsoleta. Ph. fulviventris, Ph. pruinosa. Dlplotaxi superflua, Anomala subaenea, Cyclocephala freudei y C. lunulata. Esta fauna tiene un .35% de similitud específica con Chamela, Jalisco y 33%)o de similirud específica con la región sur del estado de Morelos, y representa al 8.3% de las especies de Lamellicornia citadas para México.
Analysis on network performance of container virtualization on IoT devices (2017)

Container-based virtualization offers several advantages such as high performance, resource efficiency, and agile environment, which can make IoT device management easy. Though previous studies introduce container-based virtualization to IoT devices, they do not address different network modes of containers and the performance issues. Because the network performance is an important factor in IoT, analysis on the network performance of containers is essential. In this paper, we analyze the network performance of containers by deploying container-based virtualization on an actual IoT device, Raspberry pi3.
Investigation of the ring-closing metathesis of peptides in water (2012)

A systematic study of the ring-closing metathesis (RCM) of unprotected oxytocin and crotalphine peptide analogues in water is reported. The replacement of cysteine with S-allyl cysteine enables RCM to proceed readily in water containing excess MgCl(2) with 30% t-BuOH as a co-solvent. The presence of the sulfur atom is vital for efficient aqueous RCM to occur, with non-sulfur containing analogues undergoing RCM in low yields.
Role of Molecular Order and Solid-State Structure in Organic Field-Effect Transistors (2011)

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRole of Molecular Order and Solid-State Structure in Organic Field-Effect TransistorsMarta Mas-Torrent* and Concepció Rovira*View Author Information Institut de Ciència de Materials de Barcelona (ICMAB), Consejo Superior de Investigacions Científicas (CSIC), Campus UAB, 08193 Bellaterra, Spain Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), ICMAB-CSIC, 08193 Bellaterra, SpainPhone: +34 935801853. Fax: +34 935805729. E-mail: [email protected] (M.M.-T.); [email protected] (C.R.).Cite this: Chem. Rev. 2011, 111, 8, 4833–4856Publication Date (Web):March 18, 2011Publication History Received14 May 2010Published online18 March 2011Published inissue 10 August 2011https://pubs.acs.org/doi/10.1021/cr100142whttps://doi.org/10.1021/cr100142wreview-articleACS PublicationsCopyright © 2011 American Chemical SocietyRequest reuse permissionsArticle Views11605Altmetric-Citations492LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Crystal structure,Crystals,Mobility,Molecules,Thin films Get e-Alerts
Enhanced Electrical Properties of SrTiO3 Thin Films Grown by Plasma-Enhanced Atomic Layer Deposition (2011)

SrTiO3 (STO) thin films were grown on a Si substrate and a TiN electrode by a plasma-enhanced atomic layer deposition (PEALD) process using oxygen plasma. The amorphous STO thin films deposited by the PEALD process showed excellent step coverage, in excess of 97%, and no interfacial layer was detected. The electrical properties of the TiN/STO/TiN capacitor structures were measured by capacitance-voltage (C-V) testing after low temperature annealing in an oxygen atmosphere at 500°C for 10 min. These conditions resulted in a product with the highest dielectric constant value (∼130).
Non-linear control of a grid-connected multi-cell photovoltaic inverter that operates under variable temperature and irradiance (2015)

This work evaluates the performance of a nonlinear control strategy of a modular photovoltaic (PV) plant that is used to support grid frequency control. The PV plant is designed based on a real study case and it has a modular structure consisting in several generating units. Each generating unit consists in PV arrays interfaced to the grid with a three-phase three-level cascaded H-bridge converter based on single-phase current-source inverters. It is shown that, under changing temperature and irradiance conditions, the output dc currents of the PV arrays become unbalanced. A non-linear strategy is proposed to control the output voltages of the H-bridges and to overcome the problem of unbalanced dc currents. Additionally a control technique called virtual synchronous generator (VSG) is applied to the PV plant in order to support the control of the grid frequency emulating an inertial response and providing damping and droop power. For the simulated cases, the nonlinear strategy was always capable of keeping the output voltages balanced. However, when the PV plant performed the VSG functions, the nonlinear strategy demonstrated a poor tracking capability of the output power reference. These results suggest that higher order controllers (instead of only gains) have to be tested in the nonlinear strategy in order to improve the tracking of the VSG power reference.
Major histocompatibility complex class II<sup>–</sup> fetal skin dendritic cells are potent accessory cells of polyclonal T‐cell responses (2000)

Whereas dendritic cells (DC) and Langerhans cells (LC) isolated from organs of adult individuals express surface major histocompatibility complex (MHC) class II antigens, DC lines generated from fetal murine skin, while capable of activating naive, allogeneic CD8+ T cells in a MHC class I-restricted fashion, do not exhibit anti-MHC class II surface reactivity and fail to stimulate the proliferation of naive, allogeneic CD4+ T cells. To test whether the CD45+ MHC class I+ CD80+ DC line 80/1 expresses incompetent, or fails to transcribe, MHC class II molecules, we performed biochemical and molecular studies using Western blot and polymerase chain reaction analysis. We found that 80/1 DC express MHC class II molecules neither at the protein nor at the transcriptional level. Ultrastructural examination of these cells revealed the presence of a LC-like morphology with indented nuclei, active cytoplasm, intermediate filaments and dendritic processes. In contrast to adult LC, no LC-specific cytoplasmic organelles (Birbeck granules) were present. Functionally, 80/1 DC in the presence, but not in the absence, of concanavalin A and anti-T-cell receptor monoclonal antibodies stimulated a vigorous proliferative response of naive CD4+ and CD8+ T cells. Furthermore, we found that the anti-CD3-induced stimulation of naive CD4+ and CD8+ T cells was critically dependent on the expression of FcgammaR on 80/1 DC and that the requirement for co-stimulation depends on the intensity of T-cell receptor signalling.
Integrating Reservoir and Geomechanical Models to Compare the Productivity of Shale Reservoirs Using Different Fracture Techniques (2013)

Abstract The ultra-tight nature of shale gas reservoirs has made hydraulic fracturing inevitable. Whilst most of the gas industry is driven by the success of hydraulic fracturing, alternate fracturing techniques have repeatedly been overlooked. In hydraulic fracturing, only 20–30% of the injected water is recovered during clean-up, and this causes a concern over the fracture efficiency. Additionally, the overbalanced pressure during fracturing poses a threat towards damaging the fracture sand face. A potential alternative to eliminate these drawbacks is a forgotten technology-pulsed gas fracturing. The pulsed fracturing (through high pressure gas) also makes the clean-up much faster/simpler. Although the stimulated reservoir volume (SRV) resulting from pulsed fracturing might not be comparable to the one resulting from hydraulic fracturing, it is suspected that the former technique would be more efficient especially in ductile shale where the industry lacks efficient stimulation technique as hydraulic fracturing fails to be successful. Hydraulic fracturing involves a relatively slow loading rate and this usually results in bi-wing fracture geometries. On the other hand, explosive fracturing involves very rapid loading of formation that can trigger multiple fractures. However, due to extreme stress/heat that is generated during the explosion, near wellbore region may reach the plastic limit which inhibits fracture growth away from the wellbore. The pulsed fracturing loading rate and peak load (via high energy gas or propellants) lies in between hydraulic and explosive fracturing processes. This technique has the potential to shatter ductile shale(s): in particular by triggering a ductile to brittle transition: at an optimized pulse rate at a certain depth. Through coupling of geomechanical and reservoir models, a workflow to predict the generated fracture networks and to quantify the productivity/efficiency of the same has been established. Additionally, in order to account for the various phenomena that are expected to occur during transient production, parameters such as desorption and stress dependent permeability have been considered and are presented in this paper. This paper serves as a platform to compare the models depicting the established process of hydraulic fracturing versus an alternate fracturing technique like pulsed gas fracturing.
The clarification of a discrepancy in descriptions of maxillary musculature in larval Simuliidae (1968)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
A Set of Measures of Centrality Based on Betweenness (1977)

A family of new measures of point and graph centrality based on early intuitions of Bavelas (1948) is introduced. These measures define centrality in terms of the degree to which a point falls on the shortest path between others and there fore has a potential for control of communication. They may be used to index centrality in any large or small network of symmetrical relations, whether connected or unconnected.
Women at the top (1989)

As a woman and a nurse in what is often a demanding job I can feel empathy with Shirley Goodwin’s decision to resign from the Health Visitors Association. Shirley has been frank and honest in airing her feelings about women in key positions forced to leave because of the immense workload and stress associated with their jobs.
A Large Scale Process for the Preparation of Thymitaq (2008)

The large scale manufacturing of the anticancer agent 2-amino-6-methyl-5-(pyridin-4-ylsulfanyl)-3H-quinazolin-4-one dihydrochloride (thymitaq) from 6-bromo-5-methylanthranilic acid is described. The chemical route consists of two chemical steps: formation of a bromoquinazolinone and a copper-mediated Ullman-like coupling between 4-mercaptopyridine and the bromoquinazolinone. During process development, sodium hydride was replaced with sodium hydroxide and a method for removal of copper, based on 2,4,6-trimercapto-s-triazine, was developed. A number of purification operations were performed to ensure a product of pharmaceutical quality.
Measurement of inter and intra fraction organ motion in radiotherapy using cone beam CT projection images (2008)

A method is presented for extraction of intra and inter fraction motion of seeds/markers within the patient from cone beam CT (CBCT) projection images. The position of the marker is determined on each projection image and fitted to a function describing the projection of a fixed point onto the imaging panel at different gantry angles. The fitted parameters provide the mean marker position with respect to the isocentre. Differences between the theoretical function and the actual projected marker positions are used to estimate the range of intra fraction motion and the principal motion axis in the transverse plane. The method was validated using CBCT projection images of a static marker at known locations and of a marker moving with known amplitude. The mean difference between actual and measured motion range was less than 1 mm in all directions, although errors of up to 5 mm were observed when large amplitude motion was present in an orthogonal direction. In these cases it was possible to calculate the range of motion magnitudes consistent with the observed marker trajectory. The method was shown to be feasible using clinical CBCT projections of a pancreas cancer patient.
Air Quality Monitoring using a Whole-Cell based Sensor System (2011)

In this work, a novel whole-cell based biosensor for the detection of toxic substances in air was established. Due to their basic necessity of liquid environment, cell-based biosensors were underrepresented in the field of gas sensors in the last decades. The adaption of a commercial sensor chip (Bionas®) for the measurement of pollutants in liquids enables the direct exposure of cells with air. Cells of the respiration tract (A549, RPMI2650, V79), which tend to survive at a gas phase, are used as biological receptors. Three physiological cell parameters are monitored continuously in parallel (acidification, respiration, morphology). Water insoluble gases (e.g. CO) as well as water soluble gases (e.g. NH3) were used as model gases to test the feasibility of the novel sensor system. MIR-measurements proofed the reproducibility of the draining method. This sensor system provides a basis for many sensing applications such as environmental monitoring, building technology and public security. © 2011 Published by Elsevier Ltd.
The Structure and Specificity of <i>Escherichia coli </i>Maltose Acetyltransferase Give New Insight into the LacA Family of Acyltransferases<sup>,</sup> (2003)

The crystallographic three-dimensional structure of the Escherichia coli maa gene product, previously identified as a maltose O-acetyltransferase (MAT) [Brand, B., and Boos, W. (1991) J. Biol. Chem. 266, 14113-14118] has been determined to 2.15 A resolution by the single anomalous dispersion method using data from a crystal cocrystallized with trimethyllead acetate. It is shown here that MAT acetylates glucose exclusively at the C6 position and maltose at the C6 position of the nonreducing end glucosyl moiety. Furthermore, MAT shows higher affinity toward artificial substrates containing an alkyl or hydrophobic chain as well as a glucosyl unit. The presence of a long hydrophobic patch near the acceptor site provides the structural explanation for this preference. The three-dimensional structure reveals the expected trimeric left-handed parallel beta-helix structure found in all other known hexapeptide repeat enzymes. In particular, the structure shows similarities both overall and at the putative active site to the recently determined structure of galactoside acetyltransferase (GAT), the lacA gene product [Wang, X.-G., Olsen, L. R., and Roderick, S. L. (2002) Structure 10, 581-588]. The structure, together with the new biochemical data, suggests that GAT and MAT are more closely related than previously thought and might have similar cellular functions. However, while GAT is specific for acetylation of galactosyl units, MAT is specific for glucosyl units and is able to acetylate maltooligosaccharides, an important property for biotechnological applications. Structural differences at the acceptor site reflect the differences in substrate specificity.
Iron-regulated outer membrane protein of Bacteroides fragilis involved in heme uptake (1990)

Under iron starvation, Bacteroides fragilis expresses various iron-regulated outer membrane proteins. In this study, a deferrated minimal medium was used in growth experiments, and the role of one of these iron-regulated outer membrane proteins (a 44-kDa protein) in an iron uptake mechanism which acquires iron from heme compounds was elucidated. When a specific 44-kDa protein antiserum was used in a medium with heme as the only iron source, growth inhibition was observed. These results demonstrate that the 44-kDa outer membrane protein plays an important role in the uptake of heme in B. fragilis.
Secure architecture for RFID enabled supply chain hierarchy (2010)

Radio Frequency Identification (RFID) tags possess the ability to uniquely identify every individual item at low cost. This emerging RF technology is well suited for supply chain management and is expected to replace barcodes in the near future. However, unlike barcodes, these tags have an extended range in which they are allowed to be queried by a reader. This may result in different attack scenarios such as unauthorized scanning by malicious readers Therefore, a security protocol for RFID enabled supply chain is necessary to ensure the privacy and authentication between each tag, the corresponding reader and the backend server. In order to counter the evolving security issues, we propose a secure RFID based architecture for supply chain hierarchy. This architecture comprises of four different phases depicting the movement of a tagged item from source to destination. There are four different protocols supporting this architecture. Although this architecture was designed for use in supply chains, it can also be used for other RFID enabled applications by incurring minute changes.
Retorno absoluto: entre el mito y la realidad (2011)

The development of microwave absorbing materials with strong absorption capacity, wide bandwidth and light weight has always been a topic of concern. Herein, one-dimensional (1D) M (Co, Ni)@polyaniline (PANI) nanochains (NCs) with adjustable thickness have been successfully synthesized by reducing the mental ions under a parallel magnetic field, pretreating metal nanochains with KH550 and pre-oxidization of aniline monomer. It is found that Co has a more favorable absorption width for electromagnetic waves (EMW) and Ni aims at the absorption intensity. Furthermore, the effect of metal elements on adjusting impedance matching is more significant than their magnetic loss for composites. The minimum reflection loss (RLmin) of CoP2 can be up to -73.16 dB at 4.63 mm and the effective absorption bandwidth (EAB) is 4.98 GHz at 2.17 mm, while those of NiP2 are -65.06 dB at 3.88 mm and 5.02 GHz at 2.05 mm. The increase of PANI content can significantly reduce the matching thickness. And the RLmin of CoP3 and NiP3 can reach -58.72 dB at 2.32 mm and -65.96 dB at 1.59 mm, respectively. The absorption mechanism reveals that the matching thickness of the quarter-wavelength determines frequency location. And high absorption intensity is attributed to the synergistic effects of impedance matching, conduction loss, polarization loss, and magnetic loss. This work provides a theoretical basis for designing PANI or other conducting polymers coating magnetic nanochains for electromagnetic absorbing materials with strong absorption capacity, wide bandwidth and light weight.
The red cell membrane and its cytoskeleton (1981)

Research Article| July 15 1981 The red cell membrane and its cytoskeleton W B Gratzer W B Gratzer Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1981) 198 (1): 1–8. https://doi.org/10.1042/bj1980001 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Cite Icon Cite Get Permissions Citation W B Gratzer; The red cell membrane and its cytoskeleton. Biochem J 15 July 1981; 198 (1): 1–8. doi: https://doi.org/10.1042/bj1980001 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu nav search search input Search input auto suggest search filter All ContentAll JournalsBiochemical Journal Search Advanced Search This content is only available as a PDF. © 1981 London: The Biochemical Society1981 Article PDF first page preview Close Modal You do not currently have access to this content.
Transient expressions of synthetic biology in plants (2014)

Recent developments in transient expression methods have enabled the efficient delivery and expression of multiple genes within the same plant cell over a timescale of days. In some cases, the vectors deployed can be fine-tuned to allow differential expression of the various genes. This has opened the way to the deployment of transient expression for such applications as the production of macromolecular complexes and the analysis and manipulation of metabolic pathways. The ability to observe the effect of gene expression in a matter of days means that transient expression is becoming the method of choice for many plant-based synthetic biology applications.