gebhart/patrepeval huggingface dataset:
Monovision Laser in situ Keratomileusis for Pre-presbyopic and Presbyopic Patients (2004)

ABSTRACT PURPOSE: To evaluate the outcome and success of refractive surgery in pre-presbyopic and presbyopic patients with monovision after laser in situ keratomileusis (LASIK). METHODS: We performed a retrospective analysis of 748 eyes of 374 patients over 35 years of age treated in one or both eyes for a monovision outcome using the Alcon LADARVision 4000 laser. Analysis of age at the time of surgery, gender, bilateral versus unilateral treatment, targeted outcome, and patient acceptance are reported. RESULTS: The success rate of monovision patients in this study was 92.5%. Acceptance rate was higher in older patients, although there was no significantly greater acceptance of monovision for any of the variables studied (P values >.26). CONCLUSIONS: Monovision is a valuable option for pre-presbyopic and presbyopic patients considering refractive surgery. [J Refract Surg 2004;20: 325-328]
Antifungal activity of volatile metabolites emitted by mycelial cultures of saprophytic fungi (2011)

The antifungal activity of molecular identified Chilean saprobiontic fungi, Trichoderma viride, Schizophyllum commune and Trametes versicolor, on the fungal plant pathogens Botrytis cinerea and Fusarium oxysporum, and the saprotrophic mould Mucor miehei was investigated using two types of inhibition bioassay: (1) bi-compartmented Petri dishes and (2) two Erlenmeyer flasks connected by their upper parts. The chemical composition of volatile organic compounds (VOCs) released by saprobiontic fungi was also investigated using headspace solid-phase microextraction (SPME) and gas chromatography/mass spectrometry (GC-MS) analysis. Of the saprobiontic fungi evaluated, one isolate of S. commune showed the highest inhibitory activity against B. cinerea and M. miehei, 86.0±5.4 and 99.5±0.5% respectively. The volatile profiles of fungal isolates were shown to contain a different class of compounds. The major components in the headspace of mycelial cultures were 6-pentyl-α -pyrone (T. viride), ethanol and β -bisabolol (S. commune), and a sesquiterpene alcohol (Tr. versicolor). This is the first study reported on the release of VOCs by Chilean native fungi and their antifungal activity wrt. plant pathogenic fungi.
Irradiance from Distinct Wavelength Light-emitting Diodes Affect Secondary Metabolites in Kale (2008)

The use of light-emitting diodes (LEDs) for plant production is a new field of research that has great promise to optimize wavelength-specific lighting systems for precise management of plant physiological responses and important secondary metabolite production. In our experiment, hydroponically cultured kale plants ( Brassica oleracea L. var. acephala D.C.) were grown under specific LED wavelength treatments of 730, 640, 525, 440, and 400 nm to determine changes in the accumulation of chlorophylls, carotenoids, and glucosinolates. Maximum accumulation, on a fresh mass basis, of chlorophyll a and b and lutein occurred at the wavelength of 640 nm, whereas β-carotene accumulation peaked under the 440-nm treatment. However, when lutein was measured on a dry mass basis, maximum accumulation was shifted to 440 nm. Sinigrin was the only glucosinolate to respond to wavelength treatments. Wavelength control using LED technology can affect the production of secondary metabolites such as carotenoids and glucosinolates with irradiance levels also a factor in kale. Management of irradiance and wavelength may hold promise to maximize nutritional potential of vegetable crops grown in controlled environments.
Endotoxin-Induced Maturation of MyD88-Deficient Dendritic Cells (2001)

LPS, a major component of the cell wall of Gram-negative bacteria, can induce a variety of biological responses including cytokine production from macrophages, B cell proliferation, and endotoxin shock. All of them were completely abolished in MyD88-deficient mice, indicating the essential role of MyD88 in LPS signaling. However, MyD88-deficient cells still show activation of NF-kappaB and mitogen-activated protein kinase cascades, although the biological significance of this activation is not clear. In this study, we have examined the effects of LPS on dendritic cells (DCs) from wild-type and several mutant mice. LPS-induced cytokine production from DCs was dependent on MyD88. However, LPS could induce functional maturation of MyD88-deficient DCs, including up-regulation of costimulatory molecules and enhancement of APC activity. MyD88-deficient DCs could not mature in response to bacterial DNA, the ligand for Toll-like receptor (TLR)9, indicating that MyD88 is differentially required for TLR family signaling. MyD88-dependent and -independent pathways originate at the intracytoplasmic region of TLR4, because both cytokine induction and functional maturation were abolished in DCs from C3H/HeJ mice carrying the point mutation in the region. Finally, in vivo analysis revealed that MyD88-, but not TLR4-, deficient splenic CD11c(+) DCs could up-regulate their costimulatory molecule expression in response to LPS. Collectively, the present study provides the first evidence that the MyD88-independent pathway downstream of TLR4 can lead to functional DC maturation, which is critical for a link between innate and adaptive immunity.
Composite Piezoelectric Ceramics of (LiBi)<sub>1/2</sub>-Modifed PZT System (1989)

Composite piezoelectric ceramics of (LiBi) 1/2 -modified Pb(Zr y Ti 1- y )O 3 (PZT) system are sutudied to stabilize the temperature dependence of frequency constant, N , by the mixed sintering method with the granule. Composite ceramics, with the measured density ratio over 95%, have a high electromechanical coupling factor, k p (> 50%). The temperature coefficient, TCN P , of the N p , from room temperature to 130°C, is +11 ppm/°C which is more stable than that of conventional PZT ceramics.
Terfenadine, an H <sub>1</sub> Antihistamine, Inhibits Histamine Release <i>In Vivo</i> in the Human (1990)

A double-blind, placebo-controlled clinical trial was performed on 12 subjects with allergic rhinitis to evaluate the effect of 1-wk pretreatment with terfenadine (60 mg twice daily or 300 mg twice daily) on the response to sequential nasal challenges with allergen and histamine. The response to allergen challenge was monitored by counting sneezes and by quantifying the levels of histamine, kinins, TAME-esterase activity, and albumin in recovered nasal lavages. Following histamine challenges, sneezing and the levels of albumin were recorded. Terfenadine significantly reduced sneezing and the levels of histamine, kinins, TAME-esterase activity, and albumin seen after antigen challenge. In addition, terfenadine reduced the increase in sneezing and vascular permeability, as measured by albumin levels, following challenge with histamine. No significant differences were found between the two doses of terfenadine. Our data suggest that terfenadine not only antagonizes the H1 activity of released histamine during the immediate allergic reaction but also, at currently prescribed doses, inhibits the release of histamine.
Attainable and Relevant Moral Exemplars Are More Effective than Extraordinary Exemplars in Promoting Voluntary Service Engagement (2017)

The present study aimed to develop effective moral educational interventions based on social psychology by using stories of moral exemplars. We tested whether motivation to engage in voluntary service as a form of moral behavior was better promoted by attainable and relevant exemplars or by unattainable and irrelevant exemplars. First, experiment 1, conducted in a lab, showed that stories of attainable exemplars more effectively promoted voluntary service activity engagement among undergraduate students compared with stories of unattainable exemplars and non-moral stories. Second, experiment 2, a middle school classroom-level experiment with a quasi-experimental design, demonstrated that peer exemplars, who are perceived to be attainable and relevant to students, better promoted service engagement compared with historic figures in moral education classes.
Isolation and stabilisation of natural pigments for food applications (2006)

Purpose of review: The review attempts to highlight the relevant aspects of the isolation and stabilisation techniques used for recovering natural pigments with excellent stability for myriad food applications. Recent findings: With the recent understanding of the harmfulness of synthetic food colours, natural pigments are being increasingly emphasised and have achieved commercial significance due to the fact that consumers perceive them as safe additives. Besides aesthetics, pigments have potential physiological effects due to their potent antioxidant properties. Nevertheless, these exempt colours need to be used at higher levels than their synthetic counterparts, and their low stability in food applications pose a challenge to food manufacturers. The process of selecting of a natural colourant is most often influenced by factors such as the target shade, the physical/chemical attributes of the food matrix, stability to processing and storage conditions, and regulatory issues. Directions for future work: Consideration needs to be given to the demands imposed on the industry by any changes to regulations with respect to safety issues. Continued advances in ingredient and processing technologies should see a vast array of stabilised colours in the future. Microencapsulation has great potential for protecting natural pigments, especially those that are sensitive to light. Stabilised pigments should be aimed at higher cellular uptake for effective nutraceutical benefits. Improved solubility, targetability and adhesion to tissues arise from nanosizing. In the case of plants that cannot be successfully cultivated or propagated, plant tissue cultures should be considered as an alternative method for the production of pigments. Exploring fungal chemical diversity might also be worthwhile for identifying new pigments. An intelligent screening approach, partly based on chemotaxonomy, will provide a platform for the future construction of cell factories for the production of natural water-soluble food colourants.
Influence of Porcine Spermadhesins on the Susceptibility of Boar Spermatozoa to High Dilution1 (2003)

The effect of heparin-binding and non-heparin-binding spermadhesins on the viability, motility, and mitochondrial activity of boar spermatozoa at the high dilution (300,000 sperm/ml) to which sperm are exposed during the process of sex sorting by flow cytometry was investigated. Incubation of spermatozoa with heparin-binding spermadhesins caused a time- and dose-dependent decrease in the percentage of functional spermatozoa. The percentage of viable spermatozoa incubated at 38 degrees C with heparin-binding spermadhesins diluted in PBS (1 mg/ml) dropped from 75% (0.5 h) to 4% (5 h), whereas the percentage of viable spermatozoa incubated in PBS without proteins (control) decreased from 85% (0.5 h) to 19% (5 h). Addition of non-heparin-binding PSP-I/PSP-II spermadhesin to the PBS resulted in a concentration-dependent increment of the percentage of viable cells (65% after 5-h incubation), with maximum effect at 1.5 mg/ml. The heparin-binding spermadhesins totally suppressed sperm motility and mitochondrial activity after 5 h of incubation. The same parameters of sperm incubated in the presence of 1.5 mg/ml of PSP-I/PSP-II were 50% and 58%, respectively, and the percentages of control sperm displaying motility and mitochondrial activity were 21% and 26%, respectively. Moreover, the viability, motility, and mitochondrial activity all decreased on incubation of spermatozoa with mixtures of PSP-I/PSP-II and heparin-binding spermadhesins as the concentration of the latter increased. We conclude that PSP-I/PSP-II and the heparin-binding spermadhesins exert antagonistic effects on the functionality of highly diluted boar spermatozoa. The finding that PSP-I/PSP-II contributes to maintaining sperm with high viability, motility, and mitochondrial activity for at least 5 h at physiological temperature points to its potential use as an additive for sperm preservation, specifically of highly diluted, flow-sorted spermatozoa for sex preselection.
Metabolic engineering of cobalamin (vitamin B <sub>12</sub> ) production in <i>Bacillus megaterium</i> (2009)

Cobalamin (vitamin B(12)) production in Bacillus megaterium has served as a model system for the systematic evaluation of single and multiple directed molecular and genetic optimization strategies. Plasmid and genome-based overexpression of genes involved in vitamin B(12) biosynthesis, including cbiX, sirA, modified hemA, the operons hemAXCDBL and cbiXJCDETLFGAcysG(A)cbiYbtuR, and the regulatory gene fnr, significantly increased cobalamin production. To reduce flux along the heme branch of the tetrapyrrole pathway, an antisense RNA strategy involving silencing of the hemZ gene encoding coproporphyrinogen III oxidase was successfully employed. Feedback inhibition of the initial enzyme of the tetrapyrrole biosynthesis, HemA, by heme was overcome by stabilized enzyme overproduction. Similarly, the removal of the B(12) riboswitch upstream of the cbiXJCDETLFGAcysG(A)cbiYbtuR operon and the recombinant production of three different vitamin B(12) binding proteins (glutamate mutase GlmS, ribonucleotide triphosphate reductase RtpR and methionine synthase MetH) partly abolished B(12)-dependent feedback inhibition. All these strategies increased cobalamin production in B. megaterium. Finally, combinations of these strategies enhanced the overall intracellular vitamin B(12) concentrations but also reduced the volumetric cellular amounts by placing the organism under metabolic stress.
Estimation and correlation of salivary thiocyanate levels in periodontally healthy subjects, smokers, nonsmokers, and gutka-chewers with chronic periodontitis (2016)

The present study revealed a significant increase in SCN levels in smokers with periodontitis as compared to nonsmokers.
Observation of surface undulation due to single-atomic shear of a dislocation by reflection-electron holography (1989)

A spirally deformed surface due to a single-atomic shear of a screw dislocation emerging on a GaAs(110) surface has, for the first time, been observed in the precision of \ensuremath{\sim}0.01 A\r{} by interferometrically measuring the phase of the reflected electrons. In the case of an obliquely emerging dislocation, the surface around the dislocation core was observed to be deformed rather asymmetrically than uniformly. The result can be explained by considering surface stress relaxation.
Image Sequence Denoising via Sparse and Redundant Representations (2008)

In this paper, we consider denoising of image sequences that are corrupted by zero-mean additive white Gaussian noise. Relative to single image denoising techniques, denoising of sequences aims to also utilize the temporal dimension. This assists in getting both faster algorithms and better output quality. This paper focuses on utilizing sparse and redundant representations for image sequence denoising. In the single image setting, the K-SVD algorithm is used to train a sparsifying dictionary for the corrupted image. This paper generalizes the above algorithm by offering several extensions: i) the atoms used are 3-D; ii) the dictionary is propagated from one frame to the next, reducing the number of required iterations; and iii) averaging is done on patches in both spatial and temporal neighboring locations. These modifications lead to substantial benefits in complexity and denoising performance, compared to simply running the single image algorithm sequentially. The algorithm's performance is experimentally compared to several state-of-the-art algorithms, demonstrating comparable or favorable results.
Mapping of distinct von Willebrand factor domains interacting with platelet GPIb and GPIIb/IIIa and with collagen using monoclonal antibodies (1986)

We have used monoclonal antibodies (M Abs) and proteolytic fragmentation to localize structurally the functional sites of human von Willebrand factor (vWF) responsible for interaction with membrane glycoproteins GPIb, GPIIb/IIIa, and with collagen. SpII (215 kd) and SpIII (320 kd), the S aureus V-8 protease homodimeric fragments representing the carboxy-terminal and amino-terminal segments of the vWF subunit, competitively inhibited the binding of multimeric vWF to thrombin-stimulated or ristocetin-stimulated platelets, respectively. Specific saturable binding of each fragment was observed to stimulate platelets appropriately and was inhibited only by selected M Abs that both bound to the specific fragment and inhibited the corresponding function. M Ab 9, which blocks thrombin-induced binding of vWF to platelets, inhibited binding of SpII to platelets and bound to SpII as well as to a dimeric, 86-kd thermolysin fragment composed of 42-kd and 23-kd subunits, each possessing the epitope. Binding of SpII was also inhibited by a M Ab to GPIIb/IIIa. Thus, it appears that a portion of the carboxy-terminal end of vWF contains the ligand site for the GPIIb/IIIa receptor. In contrast, M Ab H9, which blocks ristocetin-induced binding of vWF to platelets, inhibited binding of SpIII to platelets and bound to SpIII as well as to monomeric 33-kd and 28-kd subtilisin fragments. Binding of SpIII to platelets was also inhibited by a M Ab to GPIb. Thus, it appears that a small segment of the amino-terminal part of vWF contains the ligand for the platelet GPIb receptor. The collagen binding site of vWF was localized with M Ab B203, which inhibits vWF interaction with collagen. This M Ab also bound to SpIII as well as to monomeric 26-kd and 23-kd subtilisin fragments. Thus, the third functional site responsible for collagen binding appears to be localized on the amino-terminal portion of vWF, in a linear sequence different from those responsible for interaction with either of the platelet receptors. These assignments of functional sites should facilitate the localization of structural defects of vWF in the various forms of vWD and support the role of vWF as an adhesive protein with multiple interactive sites.
Learning physics-based motion style with nonlinear inverse optimization (2005)

This paper presents a novel physics-based representation of realistic character motion. The dynamical model incorporates several factors of locomotion derived from the biomechanical literature, including relative preferences for using some muscles more than others. elastic mechanisms at joints due to the mechanical properties of tendons, ligaments, and muscles, and variable stiffness at joints depending on the task. When used in a spacetime optimization framework, the parameters of this model define a wide range of styles of natural human movement.Due to the complexity of biological motion, these style parameters are too difficult to design by hand. To address this, we introduce Nonlinear Inverse Optimization, a novel algorithm for estimating optimization parameters from motion capture data. Our method can extract the physical parameters from a single short motion sequence. Once captured, this representation of style is extremely flexible: motions can be generated in the same style but performing different tasks, and styles may be edited to change the physical properties of the body.
Selenium Enrichment of Pleurotus cornucopiae (Paulet) Rolland and Grifola frondosa (Dicks.:Fr.) S.F. Gray Mushrooms (2006)

The objective of this study was to determine if Pleurotus cornucopiae (Paulet) Rolland and Grifola frondosa (Dicks.:Fr.) S.F. Gray mushrooms could be enriched with selenium by addition of sodium selenite to the growth substrate. Separate crops were grown using a cottonseed-hullor sawdust-based synthetic medium for each mushroom with various levels of selenium added by addition of appropriate amounts of sodium selenite prior to pasteurization and inoculation. Basidiomata were harvested from the treated substrates, weighed for yield determination, freeze-dried for solids determination, and then analyzed for selenium content using graphite-furnace atomic absorption spectrophotometry. Yield of P. cornucopiae was not significantly affected by addition of selenium to the substrate, while yield and growing period of G. frondosa. was reduced when higher levels of selenium were added. Selenium increased in basidiomata of both mushrooms in direct response to levels added to the substrate, but greater uptake of selenium occurred with P. cornucopiae compared to G. frondosa. These results indicate that both mushrooms can be predictably enriched with selenium to become an excellent nutritional source of selenium but more selenium would need to be added to the substrate, used to grow G. frondosa., with the same level of selenium present in the basidiomata.
Fabricating spatially-varying subsurface scattering (2010)

Many real world surfaces exhibit translucent appearance due to subsurface scattering. Although various methods exists to measure, edit and render subsurface scattering effects, no solution exists for manufacturing physical objects with desired translucent appearance. In this paper, we present a complete solution for fabricating a material volume with a desired surface BSSRDF. We stack layers from a fixed set of manufacturing materials whose thickness is varied spatially to reproduce the heterogeneity of the input BSSRDF. Given an input BSSRDF and the optical properties of the manufacturing materials, our system efficiently determines the optimal order and thickness of the layers. We demonstrate our approach by printing a variety of homogenous and heterogenous BSSRDFs using two hardware setups: a milling machine and a 3D printer.
Improved T2* Imaging without Increase in Scan Time: SWI Processing of 2D Gradient Echo (2013)

2D SWI and associated phase unwrapped images processed from standard 2D gradient-echo images were more sensitive in detecting T2* lesions and delineating normal venous structures and nonpathologic mineralization, and they also helped distinguish calcification at no additional scan time. SWI processing of 2D gradient-echo images may be a useful adjunct in cases in which longer scan times of 3D SWI are difficult to implement.
Production, characterization and use of five monoclonal antibodies to human IL-4. (1990)

We describe five murine monoclonal antibodies that are specific for human IL-4. At least three spatially distinct epitopes on the hIL-4 molecule are recognized by this panel of antibodies which allowed development of a sensitive sandwich EIA specific for hIL-4. The EIA is capable of detecting 200 pg/ml of hIL-4 and exhibits no detectable crossreactivity to seven other human cytokines examined. In addition to the EIA these antibodies are also useful in a number of other techniques for investigating hIL-4. Recombinant hIL-4 can be easily and efficiently purified by affinity chromatography using these mAbs. Western blotting with two of the antibodies detects as little as 1.8 ng/ml of hIL-4 in a 50 microliters sample. Finally, we present a method for cytoplasmic localization of hIL-4 by a fluorescence staining method utilizing a hIL-4 specific mAb.