gebhart/patrepeval huggingface dataset:
Nicotinamide mononucleotide adenylyltransferase. Molecular and enzymic properties of the homogeneous enzyme from bakers' yeast (1986)

Nicotinamide mononucleotide (NMN) adenylyltransferase has been purified to homogeneity from baker's yeast crude extract. The purification procedure is relatively simple and consists of high-salt extraction of enzyme activity and precipitation with poly(ethylenimine), followed by ion-exchange and dye ligand chromatography separations. The final enzyme preparation is homogeneous as judged by a single Coomassie blue stainable band when run on nondenaturating and denaturating polyacrylamide gels. The native enzyme shows a molecular weight of about 200 000, calculated by gel filtration and sucrose gradient centrifugation. The protein possesses quaternary structure and is composed of four apparently identical Mr 50 000 subunits. The absorption spectrum shows a maximum at 280 nm and a minimum at 253 nm. The isoelectric point is 6.2. Amino acid composition analysis shows the presence of 28 half-cystine residues. The same result has been obtained by titrating the enzyme in denaturating conditions with Ellman's reagent after incubation with sodium borohydride. NMN adenylyltransferase is a glycoprotein containing 2% sugar, 2 mol of alkali-labile phosphate per mole of enzyme, and 1 mol of adenine moiety per mole of enzyme. Therefore, the possibility that the enzyme is ADP-ribosylated exists. The Km values for ATP, NMN, and nicotinate mononucleotide are 0.11 mM, 0.19 nM, and 5 mM, respectively. Kinetic analysis reveals a behavior that is consistent with an ordered sequential Bi-Bi mechanism. The pH optimum is in the range 7.2-8.4.
Contact-aware nonlinear control of dynamic characters (2009)

Dynamically simulated characters are difficult to control because they are underactuated---they have no direct control over their global position and orientation. In order to succeed, control policies must look ahead to determine stabilizing actions, but such planning is complicated by frequent ground contacts that produce a discontinuous search space. This paper introduces a locomotion system that generates high-quality animation of agile movements using nonlinear controllers that plan through such contact changes. We demonstrate the general applicability of this approach by emulating walking and running motions in rigid-body simulations. Then we consolidate these controllers under a higher-level planner that interactively controls the character's direction.
Two Subsets of Naive T Helper Cells with Distinct T Cell Receptor Excision Circle Content in Human Adult Peripheral Blood (2002)

During ageing thymic function declines and is unable to meet the demand for peripheral T helper (Th) cell replenishment. Therefore, population maintenance of naive Th cells must be at least partly peripherally based. Such peripheral postthymic expansion of recent thymic emigrants (RTEs) during ageing consequently should lead to loss or dilution of T cell receptor excision circles (TRECs) from a subset of naive T cells. We have identified two subsets of naive Th cells in human adult peripheral blood characterized by a striking unequal content of TRECs, indicating different peripheral proliferative histories. TRECs are highly enriched in peripheral naive CD45RA(+) Th cells coexpressing CD31 compared with peripheral naive CD45RA(+) Th cells lacking CD31 expression, in which TRECs can hardly be detected. Furthermore we show that CD31(-)CD45RA(+) Th cells account for increasing percentages of the naive peripheral Th cell pool during ageing but retain phenotypic and functional features of naive Th cells. As CD31 is lost upon T cell receptor (TCR) engagement in vitro, we hypothesize that TCR triggering is a prerequisite for homeostatically driven peripheral postthymic expansion of human naive RTEs. We describe here the identification of peripherally expanded naive Th cells in human adult blood characterized by the loss of CD31 expression and a highly reduced TREC content.
Quantitative Characterization and Prediction of On-Line Purchasing Behavior: A Latent Variable Approach (2000)

:Realizing the full potential of the on-line consumer market requires careful identification of customer needs and expectations. As research on Internet consumer behavior is still in its infancy, a quantitative framework to characterize user profiles for e-commerce has not yet been established. This study proposes a quantitative framework that uses factor analysis to identify latent factor descriptors of Internet users’ opinions on Web vendors and on-line shopping. Predictive models based on logistic discrimination and neural networks then select the factors most predictive of the propensity to buy on-line and classify Internet users accordingly. The application of this framework shows that the obtained latent factors agree in general with the major indicators identified in previous qualitative research. A small subset of the obtained factors is shown to retain the predictive power of the whole set. Neural networks are found to perform only marginally better than logistic discrimination in the task of classification.
PREPARATION OF ZnO THIN FILMS BY SPUTTERING OF THE COMPOUND IN OXYGEN AND ARGON (1966)

Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation G. A. Rozgonyi, W. J. Polito; PREPARATION OF ZnO THIN FILMS BY SPUTTERING OF THE COMPOUND IN OXYGEN AND ARGON. Appl. Phys. Lett. 1 May 1966; 8 (9): 220–221. https://doi.org/10.1063/1.1754564 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioApplied Physics Letters Search Advanced Search |Citation Search
Role for Recombinant γ-Glutamyltransferase from <i>Treponema denticola</i> in Glutathione Metabolism (2002)

Volatile sulfur compounds, including hydrogen sulfide (H(2)S), have been implicated in the development of periodontal disease. Glutathione is an important thiol source for H(2)S production in periodontal pockets. Our recent studies have delineated a pathway of glutathione metabolism in Treponema denticola that releases H(2)S. In this pathway, gamma-glutamyltransferase (GGT) has been proposed to catalyze the first step of glutathione degradation. We have cloned the gene of GGT from T. denticola, which contains an open reading frame of 726 bp encoding a protein of 241 amino acids. Transformation of this gene into Escherichia coli led to the expression of a recombinant protein. After purification by chromatography, the recombinant protein showed enzymatic activity typical of GGT, catalyzing the degradation of Na-gamma-glutamyl-4-nitroaniline (GNA) and the hydrolysis of glutathione, releasing glutamic acid or glutamine and cysteinylglycine. L-Cysteine is not a substrate of GGT. Importantly, GNA, when added to T. denticola, was able to compete with glutathione and inhibit the production of H(2)S, ammonia, and pyruvate. This was accompanied by the suppression of hemoxidative and hemolytic activities of the bacteria. Purified GGT was inactivated by TLCK (Nalpha-p-tosyl-L-lysine chloromethyl ketone) and proteinase K treatment. However, higher enzymatic activity was demonstrated in the presence of 2-mercaptoethanol and dithiothreitol. Our further experiments showed that the addition of recombinant GGT to Porphyromonas gingivalis, a bacterium without significant glutathione-metabolizing capacity, drastically increased the utilization of glutathione by the bacterium, producing H(2)S, ammonia, and pyruvate. This was again accompanied by enhanced bacterial hemoxidative and hemolytic activities. Together, the results suggest an important role for GGT in glutathione metabolism in oral bacteria.
Silane primers for epoxy adhesives (1988)

Abstract A rapid screening test is described for measuring the water resistance of epoxy bonds to glass and metals. Thin films of epoxy, cured on glass or metal coupons, were soaked in water until the films could be loosened with a razor blade. Penetration of water to the interface was almost immediate, magnifying the differences in times to failure between primed and unprimed surfaces. Comparative qualitative ratings are given for standard silane primers with typical epoxy formulations. Additional modifications of primers are suggested for optimizing the water resistance of the bonds. Test results show that proper formulation of silane adhesion promoters may provide very water-resistant bonds between epoxies and metals or glass. Keywords: Epoxiesmetalsglasssilane coupling agentsstructural adhesives
Preparation of Magnetic Fe3O4 Microspheres Using Different Surfactant and Silica-coated Magnetic Particles (2015)

Magnetic nanomaterials have received tremendous attention in the past years due to their unique magnetic responsiveness and corresponding wide applications in many fields. Herein this paper presents several methods to prepare monodisperse magnetic microspheres through a facile hydrothermal technique. The added surfactants were sodium dodecyl benzene sulfonate (SDBS), poly(vinylpyrrolidone) (PVP), trisodium citrate (TSC), and polyethylene glycol (PEG). Transmission electron microscopy (TEM) reveals the monodispersed magnetite (Fe 3 O 4 ) microspheres with narrow size distribution can be obtained using SDBS as the surfactant. In addition, Fe 3 O 4 @SiO 2 was prepared by modified Stber methods resulting in the microspheres with more smooth surface and better dispersion.
Hlf, a novel hepatic bZIP protein, shows altered DNA-binding properties following fusion to E2A in t(17;19) acute lymphoblastic leukemia. (1992)

Oncogenic conversion of transcription factors by chromosomal translocations is implicated in leukemogenesis. We report that the t(17;19) in acute lymphoblastic leukemia produces a chimeric transcription factor consisting of the amino-terminal portion of HLH proteins E12/E47 (products of the E2A gene) fused to the basic DNA-binding and leucine zipper dimerization motifs of a novel hepatic protein called hepatic leukemia factor (Hlf). Hlf, which is not normally transcribed in lymphoid cells, belongs to the recently described PAR subfamily of basic leucine zipper (bZIP) proteins, which also includes Dbp and Tef/Vbp. Wild-type Hlf is able to bind DNA specifically as a homodimer or as a heterodimer with other PAR factors. Structural alterations of the E2a-Hlf fusion protein markedly impair its ability to bind DNA as a homodimer compared with wild-type Hlf. However, E2a-Hlf can bind DNA as a heterodimer with other PAR proteins, suggesting a novel mechanism for leukemogenic conversion of a bZIP transcription factor.
Results of the automated power systems management /APSM/ program and future technology implementation (1982)

The APSM program was initiated in 1975. The purpose of this program was to develop and demonstrate the technology and benefits of autonomous operation of planetary spacecraft power systems to meet the projected requirements of future missions. Development of the APSM program was based on implementing a selected set of autonomous functions in a state-of-the-art breadboard power system. A distributed microcomputer system was developed to implement the functions. Several critical programmatic elements were identified as necessary to implement autonomous functions. These elements, including proper skill combination, well defined autonomous functions, and management of the software design and development task, were found to be more significant than hardware management. The incorporation of APSM technology in future space programs is also discussed.
Inhibition of protein synthesis <i>in vitro</i> by crotins and ricin. Effect on the steps of peptide chain elongation (1976)

The effects of crotin I and crotin II on the partial reactions of polypeptide chain elongation were investigated and compared with the known effects of ricin. Crotin II was a more powerful inhibitor than crotin I, but no qualitative differences between the two crotins were found. Rat liver ribosomes, preincubated with crotins and washed through sucrose gradients, remained inactive in protein synthesis. Among the individual steps of elongation, the peptidyltransferase reaction was unaffected by crotins, but some of the reactions that involve the interaction of elongation factors 1 and 2 with ribosomes were modified. A strong inhibition of the binding of elongation factor 2 to ribosomes and a stimulation of the elongation factor2-dependent GTP hydrolysis were observed; this indicates the formation of a very unstable elongation factor 2--GDP--ribosome complex, which, however, allows a single round of translocation to take place in the presence ofelongation factor 2 and added GTP. The elongation factor 1-dependent GTP hydrolysis was inhibited by crotins, whereas the enzymic binding of aminoacyl-tRNA, to both rat liver and Artemia salina ribosomes, was scarcely affected. In a protein-synthesizing system the inhibition by crotins and by ricin leads to a block of the nascent peptides on the ribosomal aminoacyl-tRNA site, an effect consistent with inhibition at the level of translocation. The mechanism of action of crotins appears to be very similar to that of ricin.
Reducing the overhead of dynamic compilation (2001)

Abstract The execution model for mobile, dynamically‐linked, object‐oriented programs has evolved from fast interpretation to a mix of interpreted and dynamically compiled execution. The primary motivation for dynamic compilation is that compiled code executes significantly faster than interpreted code. However, dynamic compilation, which is performed while the application is running, introduces execution delay. In this paper we present two dynamic compilation techniques that enable high performance execution while reducing the effect of this compilation overhead. These techniques can be classified as (1) decreasing the amount of compilation performed, and (2) overlapping compilation with execution. We first present and evaluate lazy compilation , an approach used in most dynamic compilation systems in which individual methods are compiled on‐demand upon their first invocation. This is in contrast to eager compilation , in which all methods in a class are compiled when a new class is loaded. In this work, we describe our experience with eager compilation, as well as the implementation and transition to lazy compilation. We empirically detail the effectiveness of this decision. Our experimental results using the SpecJVM Java benchmarks and the Jalapeño JVM show that, compared to eager compilation, lazy compilation results in 57% fewer methods being compiled and reductions in total time of 14 to 26%. Total time in this context is compilation plus execution time. Next, we present profile‐driven, background compilation, a technique that augments lazy compilation by using idle cycles in multiprocessor systems to overlap compilation with application execution. With this approach, compilation occurs on a thread separate from that of application threads so as to reduce intermittent, and possibly substantial, delay in execution. Profile information is used to prioritize methods as candidates for background compilation. Methods are compiled according to this priority scheme so that performance‐critical methods are invoked using optimized code as soon as possible. Our results indicate that background compilation can achieve the performance of off‐line compiled applications and masks almost all compilation overhead. We show significant reductions in total time of 14 to 71% over lazy compilation. Copyright © 2001 John Wiley & Sons, Ltd.
Synthesis and Evaluation of TaC:C Low-Friction Coatings (2004)

In the large family of carbon-based coatings there are members capable of providing a rare and very desirable combination of high wear resistance and low friction. The field of application for this kind of coatings is steadily increasing which, naturally, give raise to new questions and demands. Coatings of this type can be produced in a number of different ways, but the parameter influencing their properties most is the chemical composition. To facilitate investigations of the compositional impact on the synthesis and properties of coatings, a way to alloy magnetron-sputtered thin films was developed in this thesis. It does not involve the use of reactive gases or additional material sources; instead metallic foils are attached onto the magnetron target surface and thus sputtered alongside the target material. This co-sputtering route was later used to synthesize carbon coatings alloyed with Ta, Zr, W and Al in various amounts and configurations. It was shown that the co-sputtering method could be used to alloy coatings in a very simple and straightforward manner, with excellent possibilities of controlling the amount of alloying elements. The process temperature could be kept as low as 70 °C. Carbon coatings alloyed with transition metals (MeC:C) displayed polycrystalline nanocomposite structures with 5 nm metal carbide crystallites in a matrix of near-amorphous carbon. Alloying with Ta resulted in a radical 80 % reduction in friction coefficient during dry sliding in air, from 0.22 for pure carbon to 0.04 for TaC:C. This was found to be due to facilitated graphitization and the formation of lubricating surface oxides. Al additions to TaC:C resulted in a transformation of the TaC phase to a metastable, previously unreported Ta1-χAlχC. The coefficient of friction remained unaltered, but the oxidation rate compared to TaC:C was significantly reduced due to the formation of AlTaO4 instead of Ta2O5.
Improved Sap Flow Gauge for Woody and Herbaceous Plants (1990)

Abstract The internal wiring of an existing stem or trunk flow gauge was redesigned to obtain greater accuracy of the gauge itself, eliminate errors due to signal loss in connecting cables, and reduce the number of channels and of the computing required of the datalogger. Tests of the gauge conducted on bald cypress ( Taxodium distichum ) and Ficus retusa (L.) Nitida trees, in a greenhouse and in an urban backyard, and under well‐watered and dry conditions gave daily sap mass flow rates that were within 5% of those obtained by direct weighing.
Langevin dynamcis simulations of driven polymer translocation into a cross‐linked gel (2017)

We investigate the dynamics of driving a polyelectrolyte such as DNA through a nanopore and into a cross-linked gel. Placing the gel on the trans-side of the nanopore can increase the translocation time while not negatively affecting the capture rates. Thus, this setup combines the mechanics of gel electrophoresis with nanopore translocation. However, contrary to typical gel electrophoresis scenarios, the effect of the field is localized in the immediate vicinity of the nanopore and becomes negligible inside the gel matrix. Thus, we investigate the process by which a semiflexible polymer can be pushed into a gel matrix via a localized field and we describe how the dynamics of gel penetration depends upon the field intensity, polymer stiffness, and gel pore size. Our simulation results show that a semiflexible polymer enters the gel region with two distinct mechanisms depending upon the ratio between the bending length scale and the gel pore size. In both regimes, the gel fibers cause a net increase in the mean translocation time. Interestingly, the translocation rate is found to be constant (a potentially useful feature for many applications) during the predominant part of the translocation process when the polymer is stiff over a length scale comparable to the gel pore size.
Potential of Dental Mesenchymal Cells in Developing Teeth (2006)

The tooth, composed of dentin and enamel, develops through epithelium-mesenchyme interactions. Neural crest (NC) cells contribute to the dental mesenchyme in the developing tooth and differentiate into dentin-secreting odontoblasts. NC cells are known to differentiate into chondrocytes and osteoblasts in the craniofacial region. However, it is not clear whether the dental mesenchymal cells in the developing tooth possess the potential to differentiate into a lineage(s) other than the odontoblast lineage. In this study, we prepared mesenchymal cells from E13.5 tooth germ cells and assessed their potential for differentiation in culture. They differentiated into odontoblasts, chondrocyte-like cells, and osteoblast-like cells. Their derivation was confirmed by tracing NC-derived cells as LacZ(+) cells using P0-Cre/Rosa26R mice. Using the flow cytometry-fluorescent di-beta-D-galactosidase system, which makes it possible to detect LacZ(+) cells as living cells, cell surface molecules of dental mesenchymal cells were characterized. Large number of LacZ(+) NC-derived cells expressed platelet-derived growth factor receptor alpha and integrins. Taken together, these results suggest that NC-derived cells with the potential to differentiate into chondrocyte-like and osteoblast-like cells are present in the developing tooth, and these cells may contribute to tooth organogenesis.
Targeted Deletion of the Kynurenine Aminotransferase II Gene Reveals a Critical Role of Endogenous Kynurenic Acid in the Regulation of Synaptic Transmission via<i>α</i>7 Nicotinic Receptors in the Hippocampus (2004)

It has been postulated that endogenous kynurenic acid (KYNA) modulates alpha7* nicotinic acetylcholine receptor (nAChR) and NMDA receptor activities in the brain.a To test this hypothesis, alpha7* nAChR and NMDA receptor functions were studied in mice with a targeted null mutation in the gene encoding kynurenine aminotransferase II (mKat-2-/- mice), an enzyme responsible for brain KYNA synthesis. At 21 postnatal days, mKat-2-/- mice had lower hippocampal KYNA levels and higher spontaneous locomotor activity than wild-type (WT) mice. At this age, alpha7* nAChR activity induced by exogenous application of agonists to CA1 stratum radiatum interneurons was approximately 65% higher in mKat-2-/- than WT mice. Binding studies indicated that the enhanced receptor activity may not have resulted from an increase in alpha7* nAChR number. In 21-d-old mKat-2-/- mice, endogenous alpha7* nAChR activity in the hippocampus was also increased, leading to an enhancement of GABAergic activity impinging onto CA1 pyramidal neurons that could be reduced significantly by acute exposure to KYNA (100 nM). The activities of GABA(A) and NMDA receptors in the interneurons and of alpha3beta4* nAChRs regulating glutamate release onto these neurons were comparable between mKat-2-/- and WT mice. By 60 d of age, KYNA levels and GABAergic transmission in the hippocampus and locomotor activity were similar between mKat-2-/- and WT mice. Our findings that alpha7* nAChRs are major targets for KYNA in the brain may provide insights into the pathophysiology of schizophrenia and Alzheimer's disease, disorders in which brain KYNA levels are increased and alpha7* nAChR functions are impaired.
The synthesis of angiotensin-converting enzyme (ACE) inhibitors (1991)

The ACE-inhibitors constitute an important family of antihypertensive drugs. They are all chiral molecules and are all marketed as single optical isomers
Single-crystal growth by the submerged heater method (1990)

A novel method of single-crystal growth from the melt is proposed. Single crystals are grown by directional solidification in vertical bottom-seeded crucibles. The heat is supplied to the melt by an axial submerged heater located close to the growth interface. The crucible is insulated radially. The portion of the melt enclosed between the heater and the growing crystal is thermally stratified. The Rayleigh number of the enclosed melt is of the order Ra approximately 103 and remains constant during the growth process.