gebhart/patrepeval huggingface dataset:
Effects of the tom1 mutation of Arabidopsis thaliana on the multiplication of tobacco mosaic virus RNA in protoplasts (1993)

For the multiplication of RNA viruses, specific host factors are considered essential, but as of yet little is known about this aspect of virus multiplication. To identify such host factors, we previously isolated PD114, a mutant of Arabidopsis thaliana, in which the accumulation of the coat protein of tobacco mosaic virus (TMV) in uninoculated leaves of an infected plant was reduced to low levels. The causal mutation, designated tom1, was single, nuclear, and recessive. Here, we demonstrate that the tom1 mutation affects the amplification of TMV-related RNAs in a single cell. When protoplasts were inoculated with TMV RNA by electroporation, the percentage of TMV-positive protoplasts (detected by indirect immunofluorescence staining with anti-TMV antibodies) was lower (about 1/5 to 1/10) among PD114 protoplasts than among wild-type protoplasts. In TMV-positive PD114 protoplasts, the amounts of the positive-strand RNAs (the genomic RNA and subgenomic mRNAs) and coat protein reached levels similar to, or slightly lower than, those reached in TMV-positive wild-type protoplasts, but the accumulation of the positive-strand RNAs and coat protein occurred more slowly than with the wild-type protoplasts. The parallel decrease in the amounts of the coat protein and its mRNA suggests that the coat protein is translated from its mRNA with normal efficiency. These observations support the idea that the TOM1 gene encodes a host factor necessary for the efficient amplification of TMV RNA in an infected cell. Furthermore, we show that TMV multiplication in PD114 protoplasts is severely affected by the coinoculation of cucumber mosaic virus (CMV) RNA. When PD114 protoplasts were inoculated with a mixture of TMV and CMV RNAs by electroporation, the accumulation of TMV-related molecules was approximately one-fifth of that in PD114 protoplasts inoculated with TMV RNA alone. No such reduction in the accumulation of TMV-related molecules was observed when wild-type protoplasts were inoculated with a mixture of TMV and CMV RNAs or when wild-type and PD114 protoplasts were inoculated with a mixture of TMV and turnip crinkle virus RNAs. These observations are compatible with a hypothetical model in which a gene(s) that is distinct from the TOM1 gene is involved in both TMV and CMV multiplication.
Gene expression profiling using a novel method: amplified differential gene expression (ADGE) (2001)

Amplified differential gene expression (ADGE) is a novel technique, designed to profile gene expression of the whole transcriptome or to compare expression of a set of genes between two samples. ADGE employs hybridization to quadratically amplify the ratio of an expressed gene between control and tester samples before displaying. The subtle structures of adapters and primers are designed for displaying the amplified ratio of an expressed gene between two samples. Four selective nucleotides at the 3' end of primers are used to increase PCR efficiency for targeted molecules and to improve detection of PCR products. Double PCR with the same pair of primers expands the detection range, especially for genes of low abundance. Integration of these steps makes ADGE sensitive and accurate. Application to drug resistant human tumor cell lines showed that ADGE accurately profiled expression levels for induced, repressed or unchanged genes. The qualitative expression patterns for ADGE were verified with RT-PCR.
A STUDY ON THE CEMENTLIKE PROPERTIES OF MUNICIPAL WASTE INCINERATION ASHES (2003)

Combustion of municipal solid waste gives rise to four residues, including a semidry scrubber residue called reaction product (RP) and bag filter precipitator ash called fly ash (FA). This study explores the reaction of RP and FA with cement. The relations of ash wastes' alkalinity and salinity with lead leachability are correlated with composition. It is found that these residues approximate in some respects the composition of Portland or high-alumina cements. A linear dependent relationship between lead leachability and CaO/SiO2 ratios is found in FA, but leachability of RP has a linear dependence on CaO/Al2O3.
Detection of metastatic breast carcinoma with monoclonal antibodies to cytokeratins. (1989)

The presence of axillary metastases in carcinoma of the breast is of major prognostic significance. The avidin-biotin complex immunohistochemical method was used to determine if a monoclonal antibody cocktail (AE1/AE3) to cytokeratins was as specific and sensitive in detecting metastases as routine light microscopic examination of hematoxylineosin (HE)-stained sections. This study was unique in that identical sections were examined by both standard HE and immunohistochemical methods. Ninety hyperplastic axillary lymph nodes, removed from 14 female patients for a variety of diagnostic reasons, demonstrated no epithelial cells by either technique. Six of 42 nodes removed from five patients with breast cancer and known axillary metastases demonstrated tumor cells when examined with HE, whereas 13 of these nodes demonstrated cytokeratin-positive metastases. The immunohistochemical detection of cytokeratin-positive axillary metastases is both specific and sensitive.
Inferential Theory of Learning and Inductive Databases (2003)

This research was performed at the Machine Learning and Inference Laboratory at George Mason University. Laboratory's research activities are supported in part by the National Science Foundation under Grants No. IIS-9906858 and IIS-0097476, and in part by the UMBC/LUCITE #32 grant.
Sylos Labini Oligopoly Theory: Diversity of Interpretations and Possible Extensions (2007)

This paper looks at the reception and interpretation of Paolo Sylos Labini's theory of oligopoly since its inception. The most striking feature of the history of this reception is that, following a series of successive reinterpretations, the scope of his approach was gradually reduced to the point that his original message was practically forgotten, and eventually lost, notably in the context of modern approaches to microeconomic industrial theory. Contrary to what a naa¯ve observer might assume, this disappearance is not the result of progress in economic analysis, but rather of the evolution, and eventual consolidation, of the analysis of oligopolies around a specific research program
Recovery of Renal Function among ESRD Patients in the US Medicare Program (2013)

Renal recovery rates in the US Medicare ESRD program are higher than previously reported and appear to have significant geographic variation. Patients with diagnoses associated with acute kidney injury who are initiated on long-term hemodialysis have significantly higher rates of renal recovery than the general ESRD population and lower rates of permanent access placement.
Nucleophilic and radical addition of H<sub>2</sub>S to methyl linolenate and linseed oil (1976)

Abstract Hydrogen sulfide was added to methyl linolenate and linseed oil to yield mercapto, thio, and thiolan derivatives. Nucleophilic conditions at low temperatures gave faster and more complete reactions than did free radical conditions. Major reaction products were identified by gas liquid chromatography, mass spectrometry, and nuclear magnetic resonance. Nucleophilic addition of hydrogen sulfide to the methyl esters of hydrogenated nonconjugatable linseed oil was made at −70 C in the presence of boron trifluoride, and thiolan derivatives appeared to form in preference to thiane derivatives.
Focal Adhesion Kinase Activity Is Required for Bone Morphogenetic Protein—Smad1 Signaling and Osteoblastic Differentiation in Murine MC3T3-E1 Cells (2001)

Cell-matrix interactions via integrins are essential for osteoblastic differentiation. We have shown that signals activated by aggregation of alpha2beta1-integrin with type I collagen are involved in the differentiation of osteoblastic MC3T3-E1 cells. Focal adhesion kinase (FAK) is an immediate downstream signal of the beta1-integrin, and inactivation of FAK has been shown to disrupt osteoblastic differentiation. To elucidate roles of FAK in osteoblastic cells, we examined MC3T3-E1 cells stably expressing antisense FAK (asFAK) messenger RNA (mRNA). Alkaline phosphatase (ALP) activity, an osteoblastic marker, did not increase in asFAK cells with a long-term culture until 21 days or in response to bone morphogenetic protein 2 (BMP-2). Treatment with BMP-2 also failed to stimulate the expression of osteocalcin in asFAK cells. In control MC3T3-E1 cells, BMP-2 induced translocation of Smad1 into nuclei to stimulate transcriptional activity of the Smad6 promoter gene that contains a Smad1 response element. In contrast, BMP-2 failed to increase transcriptional activity of Smad6 promoter gene in asFAK cells, although it induced nuclear translocation of Smad1. These results indicate that FAK was involved in Smad1-dependent transcriptional activity but not in nuclear translocation of Smad1 in osteoblastic cells. Hence, FAK activation by integrins might converge transcriptional activation by BMP of its target genes in osteoblastic cells. These observations suggest that FAK activity is essential for BMP-Smad signaling to stimulate osteoblastic differentiation.
Effect of bran particle size on stool weight. (1978)

The increase in stool weight after feeding 20 g (dry weight) of bran daily was measured when this was of large particle size and after milling to small particle size. Twenty-eight investigations were carried out in 21 normal subjects. With coarse bran, stool weight was significantly greater than with the fine bran (mean 219.4 g/d coarse bran, 199-0 g/d fine bran: difference 20.4 g SE +/-6.4, P less than 00.1). The coarse bran also had a greater water-holding capacity (7.3 g water/g coarse bran, 3.9 water/g fine bran). Coarse bran was 2 1/2 times the volume of fine gran for a given weight and more fine bran will, therefore, be taken when bran is prescribed by the spoonful.
Hyperspectral imaging of cells: toward real-time pathological assessment (2003)

The goal of this work is to develop micro-scale noninvasive photonic instrumentation and techniques that will enable real-time imaging and monitoring of microstructures and cells in tissues. We utilize a hyperspectral microscope to explore the differences of native fluorescence and polarized light scattering from cellular components using various excitation wavelengths. The key optical “signature” characteristics that differentiate the various cellular components are used to obtain composite images that highlight their presence and the relative concentration of various tissue chromophores. The sensitivity has been optimized in our specially designed instrumentation so that image acquisition times are very short for real-time application in a clinical setting. This technology is not invasive to the cells and therefore it can be used to monitor their function while they are still alive.
Mucinous Cystadenomas and Intraductal Papillary Mucinous Tumors of the Pancreas in Magnetic Resonance Cholangiopancreatography (2000)

MRCP provides visualization of pancreatic ducts, extraductal variations, and cystic formations more completely than ERCP does. It avoids complications seen in ERCP. MRCP may replace ERCP in the evaluation of mucin-producing tumors of the pancreas.
A Method for Constructing the C2−C12 Dispiroacetal Moiety of Altohyrtin A (1996)

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTA Method for Constructing the C2−C12 Dispiroacetal Moiety of Altohyrtin AMichelle M. Claffey and Clayton H. HeathcockView Author Information Department of Chemistry, University of California, Berkeley, California 94720 Cite this: J. Org. Chem. 1996, 61, 22, 7646–7647Publication Date (Web):November 1, 1996Publication History Received23 July 1996Published online1 November 1996Published inissue 1 January 1996https://pubs.acs.org/doi/10.1021/jo9613904https://doi.org/10.1021/jo9613904rapid-communicationACS PublicationsCopyright © 1996 American Chemical SocietyRequest reuse permissionsArticle Views489Altmetric-Citations59LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-AlertscloseSupporting Info (1)»Supporting Information Supporting Information SUBJECTS:Aldehydes,Anions,Ethers,Ketones,Molecular structure Get e-Alerts
The Cu–Sn phase diagram, Part I: New experimental results (2012)

Phase diagram investigation of the Cu-Sn system was carried out on twenty Cu-rich samples by thermal analysis (DTA), metallographic methods (EPMA/SEM-EDX) and crystallographic analysis (powder XRD, high temperature powder XRD). One main issue in this work was to investigate the high temperature phases beta (W-type) and gamma (BiF<sub>3</sub>-type) and to check the phase relations between them. In the high temperature powder XRD experiments the presence of the two-phase-field between the beta- and the gamma-phase could not be confirmed. Detailed study of primary literature together with our experimental results leads to a new phase diagram version with a higher order transformation between these two high temperature phases. The present work is designated as part I of our joint publication. The new findings described here have been included into a completely new thermodynamic assessment of the Cu-Sn phase diagram which is presented in part II.
Surface plasmon resonance for characterization of large-area atomic-layer graphene film (2016)

Characterization of large-area thin films with atomic-scale resolution is challenging but in great demand for diverse applications (e.g., nanotechnology and sensing). Here, we use the Surface Plasmon Resonance (SPR) method to characterize both the thickness and refractive index of chemical-vapor-deposition (CVD)-grown graphene films. The measured refractive index and extinction coefficient values of the CVD-grown graphene monolayer at 670 nm wavelength are 3.135 and 0.897, respectively. Our results demonstrate that SPR shifts generated by graphene films are large (i.e., ∼1°/nm), almost tenfold larger than that observed in SPR measurements of organic monolayers. We find that this significantly large SPR shift easily enables the thickness of a large area sample (i.e., ∼mm2) to be determined with subnanometer-scale resolution. We show that the SPR method can identify thickness of different graphene layers and give an estimate of ∼0.37  nm for the thickness of the CVD-grown graphene layer, which agrees extremely well with the 0.335 nm reported for layer-to-layer carbon atom distance of graphite crystals. Our results open the avenue to fast and cost-effective simultaneous characterization of various parameters (including thickness and optical constants) of thin films at the atomic-scale resolution. The presented characterization method can be applied both to physical characterizations of various two-dimensional layered materials as well as to the use of these layered materials for biosensing applications as shown earlier, due to the favorable properties of graphene plasmons.
A Collection of Papers from FirstPerson, Inc. * (1995)

The papers included in this collection are the results of work conducted at the prior FirstPerson, Inc., the group which later produced the interactive browser, HotJava. The five papers report on experiments conducted: to determine which of several candidate user interfaces for panning us most usable and intuitive; to determine the smallest touchpad that subjects could accurately use to point to objects on a TV-sized video display; to determine the effects of various factors on users' ability to hit an animated character (a sprite) moving across the TV screen, using a touchpad separate from the display; to determine how accurately subjects could use a touch-sensitive remote control to hit targets on a TV screen; and to determine which of several candidate pointing devices is most usable for controlling interactive TV applications. Because FirstPerson, Inc. is no longer an active business unit, and because of its close association with the Labs, this collection was printed as a courtesy by Sun Microsystems Laboratories to document the results of these experiments. *FirstPerson, Inc. existed from 1993 to 1994.
Atacicept in patients with rheumatoid arthritis and an inadequate response to methotrexate: Results of a phase II, randomized, placebo-controlled trial (2011)

The primary end point (ACR20-CRP response) was not met despite significant biologic effects of atacicept that were consistent with its proposed mechanism of action. Modest effects of atacicept were seen for some secondary efficacy end points. Treatment with atacicept raised no new safety concerns.
Haemodialysis increases QTc interval but not QTc dispersion in ESRD patients without manifest cardiac disease (2002)

Haemodialysis increases the QTc interval in ESRD patients, mainly related to rapid changes in electrolyte plasma concentrations. However, the impact on QTc dispersion is less important in the absence of significant coexisting cardiac disease.
In Vitro Multilineage Differentiation and Self-Renewal of Single Pancreatic Colony-Forming Cells from Adult C57Bl/6 Mice (2013)

In a previous study we established colony assays suitable for studying murine adult (2-4 months) pancreatic progenitor cells plated in semisolid media containing methylcellulose and extracellular matrix proteins. Using these assays, we found robust in vitro progenitor cell activities (multilineage differentiation and self-renewal) from pancreatic cells of adult mice in the CD-1 outbred background. However, it was not clear whether progenitor cell activities can be detected from inbred mice, a preferred mouse model for various genetic studies. It was also not clear whether a single cell is sufficient to self-renew. Here, we show that fluorescent activated cell sorting pancreatic CD133(+) but not CD133(-) cells from adult C57Bl/6 inbred mice are enriched for progenitor cells that self-renew and give rise to multilineage colonies in vitro. The number of cells in a colony is in proportion to its diameter. Around 60% of single handpicked 3-week-old colonies express trilineage markers, indicating most progenitors are tripotent for ductal, acinar, and endocrine lineage differentiation. Approximately 80% of primary (freshly sorted) colony-forming progenitor cells are capable of giving rise to secondary progenitors in vitro, indicating that a majority of the primary progenitors self-renew. A single cell is sufficient for self-renewal and a Wnt agonist, R-Spondin1, enhances the number of secondary progenitors from the primary progenitors. Together, our pancreatic colony assays allow quantitative analyses of progenitors at a single-cell level from inbred mice. These assays will be useful for elucidating in vitro mechanisms of pancreatic progenitor cell biology.
Secretion of nerve growth factor in cultures of glial cells and neurons derived from different regions of the mouse brain (1989)

The regional ability of central neurons and glial cells to produce nerve growth factor (NGF) was studied in vitro. NGF secretion was compared in cultures of perinatal astrocytes or embryonic neurons that were derived from various mouse brain structures. No regional differences were detected among cultures of post-natal day 2 glial cells of hippocampal, cortical, striatal, or mesencephalic origin. In all cases, levels of NGF released by the cells were very similar. They were closely correlated to the growth rate as shown by the fact that exponentially growing cells produced relatively more factor than did confluent cells, a finding in agreement with previous observations. Unlike growth-phase cells, primary astrocytes immediately plated at high cell density did not secrete any assayable factor before the 7th day of culture. Levels of NGF found during the following days remained low. In contrast, striking differences were observed among cultures of embryonic neurons. NGF was found in relatively large amounts in cultures of embryonic day 17 or 19 striatal neurons, whereas media conditioned by neurons from the mesencephalon, cortex, or septum contained much less factor. Amounts of NGF assayed in cultures of hippocampal neurons varied with the time of sampling of this brain structure. Levels of factor were significantly higher in media conditioned by embryonic day 19 neurons than in media of embryonic day 17 neurons. However, amounts of NGF found in supernatants of hippocampal neurons remained smaller than those present in cultures of striatal nerve cells. Altogether, the results suggest that, in addition to astrocytes, central neurons may also synthesize and secrete NGF in vitro and that this phenomenum is dependent on both the origin and the developmental stage of the neuronal population.
Beneficial effects of catalase or pyruvate in a most-probable-number technique for the detection of Staphylococcus aureus (1977)

The effects of the addition of catalase (EC 1.11.1.6) or pyruvate on the enumeration of Staphylococcus aureus in Trypticase soy broth with 10% NaCl were examined using a most-probable-number technique. Addition of catalase or pyruvate to the broth increased enumeration of all heat-stressed S. aureus strains tested. Increases were also observed with nonstressed cells. Catalase and pyruvate were similarly effective when added to Trypticase soy broth-10% NaCl in enumerating staphylococci naturally present in low-temperature-rendered ground-beef samples.
Time dependent expressions for delay, stop rate and queue length at traffic signals (1980)

This report is related to the intersection analysis methodology used in the SIDRA INTERSECTION software. Since the publication of this report, many related aspects of the traffic model have been further developed in later versions of SIDRA INTERSECTION. Though some aspects of this report may be outdated, this reprint is provided as a record of important aspects of the SIDRA INTERSECTION software, and in order to promote software assessment and further research. This report was originally published by the Australian Road Research Board.