gebhart/patrepeval huggingface dataset:
The Bacterial Fimbrial Tip Acts as a Mechanical Force Sensor (2011)

There is increasing evidence that the catch bond mechanism, where binding becomes stronger under tensile force, is a common property among non-covalent interactions between biological molecules that are exposed to mechanical force in vivo. Here, by using the multi-protein tip complex of the mannose-binding type 1 fimbriae of Escherichia coli, we show how the entire quaternary structure of the adhesive organella is adapted to facilitate binding under mechanically dynamic conditions induced by flow. The fimbrial tip mediates shear-dependent adhesion of bacteria to uroepithelial cells and demonstrates force-enhanced interaction with mannose in single molecule force spectroscopy experiments. The mannose-binding, lectin domain of the apex-positioned adhesive protein FimH is docked to the anchoring pilin domain in a distinct hooked manner. The hooked conformation is highly stable in molecular dynamics simulations under no force conditions but permits an easy separation of the domains upon application of an external tensile force, allowing the lectin domain to switch from a low- to a high-affinity state. The conformation between the FimH pilin domain and the following FimG subunit of the tip is open and stable even when tensile force is applied, providing an extended lever arm for the hook unhinging under shear. Finally, the conformation between FimG and FimF subunits is highly flexible even in the absence of tensile force, conferring to the FimH adhesin an exploratory function and high binding rates. The fimbrial tip of type 1 Escherichia coli is optimized to have a dual functionality: flexible exploration and force sensing. Comparison to other structures suggests that this property is common in unrelated bacterial and eukaryotic adhesive complexes that must function in dynamic conditions.
Pharmacologically active 4-oxo-4H-chromen-2-carboxylic acids. Part II. The synthesis of 4-oxo-4H-chromen-2-carboxylic acids containing a fused imidazole or oxazole ring (1970)

The synthesis of various 2-carboxychromones containing a fused imidazole or oxazole ring is described. The imidazolochromones were prepared either from appropriately substituted vic-acetylhydroxybenzimidazoles or from vic-diaminochromones; the oxazolochromones were obtained either from vic-aminohydroxychromones, or by decomposition of azidochromones in the presence of polyphosphoric acid and a carboxylic acid.
Automated biomanipulation of single cells using magnetic microrobots (2013)

Transport of individual cells or chemical payloads on a subcellular scale is an enabling tool for the study of cellular communication, cell migration, and other localized phenomena. We present a magnetically actuated robotic system capable of fully automated manipulation of cells and microbeads. Our strategy uses autofluorescent robotic transporters and fluorescently labeled microbeads to aid tracking and control in optically obstructed environments. We demonstrate automated delivery of microbeads infused with chemicals to specified positions on neurons. This system is compatible with standard upright and inverted light microscopes and is capable of applying forces less than 1 pN for precision positioning tasks.
X-ray all-sky monitoring and transient detection using a codedsphere telescope (1996)

We present a design for a hard X-ray all-sky monitor which consists of a spherical structure whose surface is partially covered by circular units which serve the dual purpose of detector and mask elements, forming a coded aperture telescope with full steradian coverage. We demonstrate that a simple practical implementation of this geometry results in an instrument which is capable of measuring the fluxes in the 10 -20 keV bandpass of the hundred brightest sources in the sky at least every two days. We also show how this instrument can be used to image burst and transient sources; obtain their positions with arcminute accuracy; and follow their light curves throughout their lifetimes.
Handbook of Antenna Design, Vol. 2 (1983)

This book presents the fundamental background theory and analytical techniques of antenna design. It deals with a very wide range of antenna types, operating from very low frequencies to millimetre waves.
A CSMA/CD compatible MAC for real-time transmissions based on varying collision intervals (2002)

This paper suggests a CSMA/CD compatible MAC protocol for real-time transmissions in a home or small office local area network. It has the key advantage of allowing devices implementing the existing Ethernet protocol such as PCs, printers and ISDN routers to operate with devices implementing the new MAC. The new MAC enables real-time traffic such as digital video and audio to be transmitted with low delay and jitter.
HEPSIN inhibits cell growth/invasion in prostate cancer cells. (2002)

Expression of HEPSIN, a type II transmembrane serine protease in prostate cancer (CaP), has been highlighted by several studies analyzing CaP-specific gene expression alterations by cDNA microarray. Evaluations of the biological functions of HEPSIN in CaP cells are warranted for better assessment of its utility as a biomarker and/or therapeutic target. In stable clones of PC-3/HEPSIN transfectants, there was a dramatic reduction in the cell growth, cell invasion, and soft agar colony formation. A higher proportion of PC-3/HEPSIN cells were in the G(2)-M phase of the cell cycle, and there was also an increase in the cell population undergoing apoptosis. Preliminary analysis of HEPSIN transfections into LNCaP and DU145 cells further revealed cell growth-inhibitory effects. These results underscore that exogenous HEPSIN expression negatively regulates cell growth in metastatic CaP cell lines. Although the cause of the biological consequence of HEPSIN overexpression in primary CaP remains to be determined, the negative cell growth-regulatory effects of HEPSIN in metastatic CaP cells reported here have unraveled possible cellular and molecular mechanisms underlying observations that link decreased/loss of HEPSIN expression with poor prognosis of CaP.
The effects of precision constraints in a backpropagation learning network (1989)

Summary form only given, as follows. A study is presented of precision constraints imposed by a hybrid chip architecture with analog neurons and digital backpropagation calculations. Conversions between the analog and digital domains and weight storage restrictions impose precision limits on both analog and digital calculations. It is shown through simulations that a learning system of this nature can be implemented in spite of limited resolution in the analog circuits and using fixed-point arithmetic to implement the backpropagation algorithm.< <ETX>></ETX>
Structural Optimization of 4-Chlorobenzoylpiperidine Derivatives for the Development of Potent, Reversible, and Selective Monoacylglycerol Lipase (MAGL) Inhibitors (2016)

Monoacylglycerol lipase (MAGL) inhibitors are considered potential therapeutic agents for a variety of pathological conditions, including several types of cancer. Many MAGL inhibitors are reported in literature; however, most of them showed an irreversible mechanism of action, which caused important side effects. The use of reversible MAGL inhibitors has been only partially investigated so far, mainly because of the lack of compounds with good MAGL reversible inhibition properties. In this study, starting from the (4-(4-chlorobenzoyl)piperidin-1-yl)(4-methoxyphenyl)methanone (CL6a) lead compound that showed a reversible mechanism of MAGL inhibition (K<sub>i</sub> = 8.6 μM), we started its structural optimization and we developed a new potent and selective MAGL inhibitor (17b, K<sub>i</sub> = 0.65 μM). Furthermore, modeling studies suggested that the binding interactions of this compound replace a structural water molecule reproducing its H-bonds in the MAGL binding site, thus identifying a new key anchoring point for the development of new MAGL inhibitors.
A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2 (2005)

Zinc finger-containing Gli proteins mediate responsiveness to Hedgehog (Hh) signaling, with Gli2 acting as the major transcriptional activator in this pathway in mice. The discovery of disease-associated mutations points to a critical role for GLI2 in human Hh signaling as well. Here, we show that human GLI2 contains previously undescribed 5' sequence, extending the amino-terminus an additional 328 amino acids. In vitro, transcriptional activity of full-length GLI2 is up to 30 times lower than that of GLI2DeltaN (previously thought to represent the entire GLI2 protein), revealing the presence of an amino-terminal repressor domain in the full-length protein. GLI2DeltaN also exhibits potent transcriptional activity in vivo: overexpression in mouse skin leads to the formation of Hh-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive Hh signaling. The discovery of this additional, functionally relevant GLI2 sequence led us to re-examine several pathogenic human GLI2 mutants, now containing the entire amino-terminal domain. On the basis of the functional domains affected by the mutations, mutant GLI2 proteins exhibited either loss-of-function or dominant-negative activity. Moreover, deletion of the amino-terminus abrogated dominant-negative activity of mutant GLI2, revealing that this domain is required for transcriptional repressor activity of pathogenic GLI2. Our results establish the presence of an amino-terminal transcriptional repressor domain that plays a critical role in modulating the function of wild-type GLI2 and is essential for dominant-negative activity of a GLI2 mutant associated with human disease.
Solar Heat Reflecting Coatings (1969)

<div class="htmlview paragraph">Tests conducted at the U. S. Army Coating and Chemical Laboratory, Aberdeen Proving Ground, indicate that some pigments are better than others in reducing heat build-up on painted surfaces from solar radiation. Special tests were also conducted to check the application of paint on automobile surfaces, using 12 automotive finishes found on 1969 models. Results reveal that both light and dark colored paints can be formulated to reduce solar radiation heat build-up without impairing the durability of the finish or adding to production costs.</div>
Endothelial cell adhesion on RGD‐containing methacrylate terpolymers (2004)

Hexyl methacrylate (HMA), methyl methacrylate (MMA), and methacrylic acid (MAA) were used as comonomers to produce a low glass transition temperature material, potentially useful in fabricating a small diameter vascular graft. Because it has been shown that grafts seeded with endothelial cells have better resistance to thrombosis, RGD-based peptide sequences were incorporated into the terpolymer. The two methods used for incorporating peptide sequences were a chain transfer reaction during polymerization, and a coupling reaction between the amine terminus of the peptide and the carboxyl groups of the MAA. Polymers were synthesized using the chain transfer reaction with peptide concentrations ranging from 1.7 to 7.0 micromol/g. Weight-average molecular weights decreased with increasing peptide concentration from 310,000 g/mol for the terpolymer without peptide, to 110,000 g/mol for a peptide concentration of 7.0 micromol/g. As a result, Young's modulus decreased with increasing peptide concentration. Terpolymers with peptides attached through a coupling reaction showed no decrease in molecular weight or mechanical properties. Confocal microscopy showed cells seeded on the RGD surfaces adhered and spread, while terpolymers with RGE sequences showed cells that were rounded and not spreading. Cell density on RGD surfaces increased with increasing peptide concentration up to a bulk peptide concentration of approximately 5 micromol/g and reached a plateau, which indicated the minimum peptide concentration necessary for maximum cell adhesion.
Pharmacology of L-660,711 (MK-571): a novel potent and selective leukotriene D<sub>4</sub> receptor antagonist (1989)

L-660,711 (3-(3-(2-(7-chloro-2-quinolinyl)ethenyl)phenyl) ((3-dimethyl amino-3-oxo propyl)thio)methyl)thio)propanoic acid is a potent and selective competitive inhibitor of [3H]leukotriene D4 binding in guinea pig (Ki value, 0.22 nM) and human (Ki value, 2.1 nM) lung membranes but is essentially inactive versus [3H]leukotriene C4 binding (IC50 value in guinea pig lung, 23 microM). Functionally it competitively antagonized contractions of guinea pig trachea and ileum induced by leukotriene (LT) D4 (respective pA2 values, 9.4 and 10.5) and LTE4 (respective pA2 values, 9.1 and 10.4) and contractions of human trachea induced by LTD4 (pA2 value, 8.5). L-660,711 (5.8 x 10(-8)M) antagonized contractions of guinea pig trachea induced by LTC4 in the absence (dose ratio = 28) but not in the presence of 45 mM L-serine borate (dose ratio less than 2). L-660,711 (1.9 x 10(-5)M) did not block contractions of guinea pig trachea induced by histamine, acetylcholine, 5-hydroxytryptamine, PGF2 alpha, U-44069, or PGD2. In the presence of atropine, mepyramine, and indomethacin, L-660,711 (1.9 x 10(-5)M) inhibited a small component of the response to antigen on guinea pig trachea but completely blocked anti-IgE-induced contractions of human trachea. L-660,711 (i.v.) antagonized bronchoconstriction induced in anesthetized guinea pigs by i.v. LTC4, LTD4, and LTE4 but did not block bronchoconstriction to arachidonic acid, U-44069, 5-hydroxytryptamine, histamine, or acetylcholine. Intraduodenal L-660,711 antagonized LTD4 (0.2-12.8 micrograms/kg)-induced bronchoconstriction in guinea pigs, and p.o. L-660,711 blocked LTD4- and Ascaris-induced bronchoconstriction in conscious squirrel monkeys and ovalbumin-induced bronchoconstriction in conscious sensitized rats treated with methysergide (3 micrograms/kg). The pharmacological profile of L-660,711 indicates that it is a potent, selective, orally active leukotriene receptor antagonist which is well suited to determine the role played by LTD4 and LTE4 in asthma and other pathophysiologic conditions.
Quantitative structure-activity relationship of photosystem II inhibitors in chloroplasts and its link to herbicidal action (1986)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTQuantitative structure-activity relationship of photosystem II inhibitors in chloroplasts and its link to herbicidal actionKenichiro Mitsutake, Hajime Iwamura, Ryo Shimizu, and Toshio FujitaCite this: J. Agric. Food Chem. 1986, 34, 4, 725–732Publication Date (Print):July 1, 1986Publication History Published online1 May 2002Published inissue 1 July 1986https://pubs.acs.org/doi/10.1021/jf00070a034https://doi.org/10.1021/jf00070a034research-articleACS PublicationsRequest reuse permissionsArticle Views96Altmetric-Citations26LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Neurotoxina tetánica: características e importancia (2010)

Resumen El Clostridium tetani es una bacteria Gram positiva, estrictamente anaerobia y no invasiva. Este microorganismo produce la enfermedad llamada tetanos, la cual se presenta cuando la toxina tetanica, producida por la bacteria en laceraciones provocadas en piel o mucosas, viaja por via hematogena o linfatica hasta el Sistema Nervioso. La neurotoxina actpua en las interneuronas GABAergicas y glicinergicas ubicadas a nivel medular en donde se une a una proteina de las vesiculas sinapticas, la sinaptobrevina II(VAMP II), inhibiendo su funsion con la membrana de la neurona presinaptica y evitando de esta manera la liberacion del neurotransmisor. Palabras clave: Tetanos, toxina tetanica, Clostridium tetani, sinapsis, interneuronas.
Neutral glycosphingolipid expression in B‐cell neoplasms (1991)

The expression of neutral glycosphingolipids (GSL) in 37 B-cell neoplasms [7 acute lymphocytic leukemia (ALL), 5 Burkitt's lymphoma (BL), 7 chronic lymphocytic leukemia (CLL), 5 diffuse, poorly differentiated lymphoma (DPDL), 6 diffuse histiocytic lymphoma (DHL), 3 hairy-cell leukemia (HCL), and 4 multiple myeloma (MM)] was examined. Patterns of expression of simple (GlcCer, LacCer) and globo-series GSL (Gb3, Gb4) were found for each tumor type. In addition, pre-B ALL expressed the neo-lacto series GSL, paragloboside, which was not significantly seen at later stages of maturation. As a group, leukemias expressed about 10 times higher ratios of simple GSL to Globo-series GSL as compared to lymphomas, regardless of stage of differentiation. Significant amounts of GSL of other series were not found except in one CLL which contained asialo-GM2. GSL phenotype in these cells was not grossly affected by cell genotype since pre-B ALL containing Philadelphia chromosome t(9q;22q) translocations were similar to other ALL; and DHL with t(8q;14q) translocations had GSL patterns similar to other DHL samples and dissimilar to GSL patterns found in Burkitt's lymphomas with t(8q;14q). Differences in GSL expression among the different types of B-cell neoplasm suggested that GSL patterns form a phenotypic map that may complement the traditional glycoprotein immunophenotypic map and contribute to our understanding of the biology of these diseases and B-cell differentiation.
Characterization of As-P double diffused drain structure (1984)

In order to improve the source-drain gated breakdown and hot electron injection in submicron channel length MOSFET devices, an As-P double-diffused (DD) drain structure is implemented. The feasibility of this device is investigated, comparing it to conventional As only devices. Oxide sidewall spacers have been successfully fabricated to insure the formation of the lightly doped for region. The fabrication process is similiar to conventional gate processing except a conformal CVD-SiO <inf xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</inf> layer is deposited after polysilicon gate definition. This layer is then reactive ion etched to form the sidewall spacers. Extensive device characterization shows reduction of gate and substrate hot electron injection, overlap capacitance, and gated breakdown. Transconductance degradation is shown to be approximately 10% for the double-diffused device at an Leff = 1.0 µm when compared to the conventional device. However, modeled results of ring oscillator performance predict an overall performance improvement of 10% for the graded junction.
The Design of Crankshaft Vibration Dampers (1990)

The crankshaft in automotive engines often requires an auxiliary damper to control resonant torsional modes of vibration. This paper outlines modern procedures and techniques used in the design of elastomer torsional vibration dampers. The design of a damper encompasses free vibration analysis, forced vibration analysis, dampers parameter optimisation as well as geometric and material specification. The contribution of the damping in the elastomer to the complex modes is illustrated by an example. The factors affecting the selection and compounding of the dampers elastomer are discussed.
0EISUA: Zero Effort Indoor Secure User Authentication (2020)

Two-factor authentication (2FA) systems implement by verifying at least two factors. A factor can be one or more of something a user knows (password, or phrase), something a user possesses (smart card, or smartphone), something a user is (fingerprint, or iris), something a user does (keystroke), or somewhere a user is (location). In a conventional 2FA system, a user is required to interacts (e.g., typing a passcode) in order to implement the second layer of authentication, which is not very user-friendly. Nowadays, smart devices (phones, laptops, tablets, etc.) can receive signals from different radio frequency technologies within range. As these devices move among networks (Wi-Fi access points, cell phone towers, etc.), they receive broadcast messages, some of which can be used to collect information. This information can be utilized in a variety of ways, such as establishing a connection, sharing information, locating devices, and, most appropriately, identifying users in range. The principal benefit of broadcast messages is that the devices can read and process the embedded information without being connected to the broadcaster. Moreover, the broadcast messages can be received only within a range of the wireless access point sending the broadcast, thus inherently limiting access to those devices in close physical proximity and facilitating many applications dependent on that proximity. In this paper, 0EISUA is proposed, a zero-effort two-factor authentication scheme based on something that is in the user's environment (ambient access points). In our research, data from the broadcast messages are utilized to implement the second authentication factor by determining whether two devices are proximate or not to ensure that they belong to the same user, in a way that requires zero interaction from the user. The new proposed system introduced in this paper is experimentally tested.
Effect of actinomycin D and cycloheximide on replication of Sindbis virus in Aedes albopictus (mosquito) cells (1988)

Production of Sindbis virus in the presence of transcription and translation inhibitors was examined in three Aedes albopictus cell lines. Addition of cycloheximide to heat-resistant Sindbis virus (SVHR)-infected mosquito cells arrested viral RNA synthesis completely, in contrast to the effects of this drug on virus-infected vertebrate cells. Production of mature virus by both SVHR (a variant commonly used as a wild-type virus) and SBamr (a mutant which is resistant to the effects of 18 h of pretreatment of vertebrate cells with actinomycin D) in mosquito u4.4, C6-36, and C7-10 cells was inhibited by 2 h of pretreatment with actinomycin D. Pretreatment with this drug for 2 h slightly enhances virus production in vertebrate cells. Treatment of mosquito cells with actinomycin D resulted in shutoff of SVHR RNA synthesis. The mutant SBamr was able to overcome the effects of actinomycin D on viral RNA synthesis and produced both 26S and 49S RNAs, even though no viral structural proteins or mature particles were produced in the presence of the drug. This result suggests that, in the presence of actinomycin, the nonstructural genes of SBamr are translated sufficiently to allow for RNA synthesis but that 26S RNA may not be translated to an extent that allows significant virus production. These data demonstrate that host components are involved in at least two distinct steps in the production of Sindbis virus in mosquito cells: (i) production of viral RNA and (ii) synthesis of viral structural polypeptides.