gebhart/patrepeval huggingface dataset:
Physical map of the Listeria monocytogenes chromosome (1992)

The circular physical map of the pathogenic bacterium Listeria monocytogenes LO28 (serovar 1/2c) was established by using pulsed-field gel electrophoresis. The L. monocytogenes chromosome contains eight NotI fragments of 1,100, 940, 400, 335, 280, 45, 30, and 20 kb in size and eight Sse8387I fragments of 860, 680, 680, 370, 335, 130, 70, and 25 kb. Therefore, the total length of the genome is 3,150 kb. To order the NotI fragments on the chromosome, we used a strategy which can be of general use. We first cloned chromosomal HindIII or EcoRI fragments in pBR322. DNA extracted from the total libraries was digested by NotI and ligated to a NotI-kanamycin resistance cassette obtained by cutting Tn5 with NotI. After transformation in Escherichia coli, kanamycin-resistant clones originating from NotI-containing EcoRI or HindIII fragments were isolated. The two EcoRI-NotI or HindIII-NotI fragments of each recombinant plasmid were isolated and used as probes on Southern blot hybridizations to identify and link the corresponding NotI fragments. Seven NotI fragments were ordered in this way. The last junction was demonstrated by partial digest analysis. All L. monocytogenes genes identified so far as well as the six rRNA operons were localized on the NotI map. Regions homologous to genes from closely related bacteria were also detected and localized. Southern blot analysis of simple Sse8387I digests or double Sse8387I-NotI digests probed with the various NotI probes allowed us to align the Sse8387I fragments and localize the single SfiI site, resulting in the establishment of the first genetic and physical map of the L. monocytogenes chromosome.
Identifying the root cause of video streaming issues on mobile devices (2015)

Video streaming on mobile devices is prone to a multitude of faults and although well established video Quality of Experience (QoE) metrics such as stall frequency are a good indicator of the problems perceived by the user, they do not provide any insights about the nature of the problem nor where it has occurred. Quantifying the correlation between the aforementioned faults and the users' experience is a challenging task due the large number of variables and the numerous points-of-failure.
Management of the acute burn wound: an overview. (1997)

Goals for managing an acute burn wound are similar to those of other wounds such that infection and scar formation are minimized, a moist wound environment is provided, and the surrounding tissue is protected from trauma. A variety of cleansing techniques are used with burn wounds, including local wound care and nonsubmersion and immersion hydrotherapy. Topical agents have significantly decreased the development of burn wound sepsis since the 1960s, and now various experimental agents are being investigated to improve wound healing. The choice of dressings depends on many patient and wound-related factors, and synthetic, biologic, and biosynthetic dressings are used to treat the different depths of burn wounds. However, skin grafts and the newer cultured skin substitutes remain the mainstay for healing a full-thickness burn wound.
Detection of the color adulteration of green table olives with copper chlorophyllin complexes (E-141ii colorant) (2011)

The present work proposes a procedure for the detection of the color adulteration of green table olives with E-141ii colorant. Addition of this colorant to table olives to obtain a green permanent color in the product is a fraudulent practice, because the FDA allows its use only in citrus-based dry beverage mixes and European Union regulations do not permit the addition of colorants to table olives. The method consists of a pigment extraction in liquid phase and subsequent analysis by HPLC-DAD. The fraudulent addition of E-141ii is determined from the detection in fruit and/or packing solution, of peaks identified as copper chlorophyllin complexes, which are compounds completely foreign to the natural pigment profile of green table olives. The proposed methodology allows controlling the color quality of green table olives.
Chapter 2. (2007)

Abstract In 2002, the first in a series of working conferences was held as a collaboration between the counseling service of Bennington College and the Austen Riggs Center's Erikson Institute. The title of the first conference was The Escalating Use of Medications by College Students: What Are They Telling Us, What Are We Telling Them?. It was meant to provide a space to explore the meaning and context of this phenomenon. The primary contexts examined were the student's familial/ cultural context and then the institutional context from which the counseling staff was attempting to respond to the student. This paper describes that initial conference and the emergent themes over the next few years.
Constitutive RNA synthesis for the yeast activator ADR1 and identification of the ADR1-5c mutation: implications in posttranslational control of ADR1. (1986)

The regulation of mRNA production for the yeast positive activator ADR1, a gene required for the expression of the glucose-repressible alcohol dehydrogenase (ADH II), was studied. ADR1 mRNA levels did not vary when yeasts were switched from glucose- to ethanol-containing medium, while ADH II expression increased 100-fold. The mRNA for the ADR1-5c allele, which augments ADH II expression 60-fold during glucose repression, was not present in greater abundance than ADR1 mRNA. Additionally, the ccr1-1 allele, which blocks ADH2 mRNA formation and partially suppresses the ADR1-5c phenotype, did not alter the levels of ADR1 mRNA. These results indicate that ADR1 is not transcriptionally controlled. To determine the character of the ADR1-5c mutation, the region containing the mutation was identified and sequenced. At base pair +683 a G-to-A transition was detected in the ADR1 coding sequence which would result in the substitution of a lysine residue for an arginine at amino acid 228. The location of the ADR1-5c mutation in the interior of the ADR1 coding sequences suggests that it enhances the activity of an extant but inactive ADR1 protein rather than increases the abundance of ADR1 by altered translation of its mRNA. The ADR1-5c mutation occurs in a region of the polypeptide corresponding to a cyclic AMP-dependent protein kinase phosphorylation recognition sequence. The potential role of reversible phosphorylation in the posttranslational regulation of ADR1 is discussed.
The Synthesis and Hydrolysis of Sulfamic Acid Derivatives (1968)

スルファミン酸アンモニウムとアルデヒド類との反応を行ない,縮合生成物の生成しやすさと構造との関係を検討した。芳香族アルデヒド類はいずれもベンジリデン型の縮合生成物を与えるが,脂肪族アルデヒド(プロピオン-,n-ブチル- ,β-フェニルブロピオン-,およびシンナムアルデヒド)類ではシンナムアルデヒドの場合をのぞき縮合生成物を与えなかった。また,シンナミリデンスルファミン酸からr-フェニルプロピルスルファミン酸[12]への還元において,[12]の収率が還元に使用した触媒量の増加につれて増すことがわかった。これらのことから縮合生成物が単離しうる程度の安定性をもつための一つの条件として,生成物が共役系の構造をもつことが必要と考えられる。ベンギリデン型の縮合生成物の還元によりベンジル型のスルファミン酸誘導体をアルデヒド類に対し48～83%の収率で合成した。また,ベンジル[6],pクロルベンジル-[10]のスルファミン酸は,加水分解により対応する第一アミンだけを与えるが,ピペロニル[7],ベラトリル-[8]のスルファミン酸はおもに第ニアミンを生ずることから,[7],[8]の加水分解にお小ては,中間に置換ベンジルヵルポニウムイオンが生成する機構が考えられる。
Phenotypic susceptibility and virological outcome in nucleoside-experienced patients receiving three or four antiretroviral drugs (2003)

Reduced phenotypic susceptibility was a significant independent risk factor for virological failure. The presence of efavirenz hypersusceptibility appeared to enhance virological responses during treatment with efavirenz in combination with NRTIs. The retrospective calculation of continuous PSS accurately identified treatment regimens containing sufficient drug activity to prevent virological failure.
A Study on Heat Transfer in Sand Molds (1982)

In order to investigate the relationship between the thermal characteristics of the various molds as green sand mold, dry sand mold, mold and shell mold, and the solidification characteristics of molten metal, the thermal analysis of rarious molds and melt were performed. The structure of Al-Castings was a/so observed. Results obtained in this experiment were as follows : 1) The heating rate of the molds was increased in the order of green sand mold, mold, dry sand mold and shell mold, On the other hand the solidification time of the melts was shortened in the order of dry sand mold castings, mold castings, green sand mold castings and shell mold castings. 2) The arrest temperature period in the heating curve of the green sand mold was resulted from the eraporation of moisture contained in mold, which was transfered to the outer side of the mold. 3) The temperature fluctuation of the melt in the shell mold was considered to be resulted from the combution heat of resin contained in the mold. 4) The amounts of heat absorption of the molds were increased in the order of dry sand mold, mold, green sand mold and shell mold. 5) The higher the solidification rate was, the longer was its shrinkage pipe and the finer its grain size.
Play Therapy: A Non-Directive Approach for Children and Adolescents (1992)

Child Therapy and Non-directive Play Therapy. Symbolic Play: Its Role in Mental Development and Play Therapy. The Process of Play Therapy. Assessment and Planning for Play Therapy. Emotional/Social Development and Play Therapy . Practice Skills and Issues. Working with Children in Statutory Settings . References
A Simple Combinatorial Method Aiding Research on Single-Walled Carbon Nanotube Growth on Substrates (2010)

Establishing fabrication methods of carbon nanotubes (CNTs) is essential to realize many applications expected for CNTs. Catalytic growth of CNTs on substrates by chemical vapor deposition (CVD) is promising for direct fabrication of CNT devices, and catalyst nanoparticles play a crucial role in such growth. We have developed a simple method called “combinatorial masked deposition (CMD)”, in which catalyst particles of a given series of sizes and compositions are formed on a single substrate by annealing gradient catalyst layers formed by sputtering through a mask. CMD enables preparation of hundreds of catalysts on a wafer, growth of single-walled CNTs (SWCNTs), and evaluation of SWCNT diameter distributions by automated Raman mapping in a single day. CMD helps determinations of the CVD and catalyst windows realizing millimeter-tall SWCNT forest growth in 10 min, and of growth curves for a series of catalysts in a single measurement when combined with real-time monitoring. A catalyst library prepared using CMD yields various CNTs, ranging from individuals, networks, spikes, and to forests of both SWCNTs and multi-walled CNTs, and thus can be used to efficiently evaluate self-organized CNT field emitters, for example. The CMD method is simple yet effective for research of CNT growth methods.
Preparation of Small‐Particle‐Size, Semiconductor CdS‐Doped Silica Glasses by the Sol–Gel Process (1990)

The sol–gel process has been applied successfully to the preparation of small‐particle‐size CdS‐doped silica glasses with a significant quantum size effect. Gels prepared through the hydrolysis of a complex solution of Si(OC 2 H 5 ) 4 and Cd(CH 3 COO) 2 ·2H 2 O were heated at 500°C, then reacted with H 2 S gas to form fine, hexagonal, CdS‐microcrystal‐doped glasses. The optical absorption edge is blue shifted by ∼0.4 eV compared with the bulk absorption value of CdS crystal. This result is interpreted in terms of a quantum‐confinement effect of small crystal size.
A dipole moment study of the electrical effect of the trifluoromethyl group (1972)

Apparent dipole moments in benzene of a series of substituted trifluoromethylbenzenes and related compounds have been determined. The magnitude of the differences between the observed moments and those calculated by vector analysis are most appropriately explained, principally, in terms of a π electron inductive effect of the trifluoromethyl group.
Enantioselective Iridium‐Catalyzed Allylic Arylation (2008)

We describe herein the development of the first iridium-catalyzed allylic substitution using arylzinc nucleophiles. High enantioselectivities were obtained from the reactions, which used commercially available Grignard reagents as the starting materials. This methodology was also shown to be compatible with halogen/metal exchange reactions. Its synthetic potential is demonstrated by its application towards the formal synthesis of (+)-sertraline.
Oxygen distribution in sputtered Nb-Ge films (1976)

A quantiative determination of the distribution of oxygen in a high-Tc (22 K) Nb-Ge film has been made using a nuclear backscattering method. The Nb/Ge ratios as a function of depth have also been determined. An apparent correlation between oxygen concentration and Nb/Ge ratio is observed.
Constructive Feedforward Neural Networks Using Hermite Polynomial Activation Functions (2005)

In this paper, a constructive one-hidden-layer network is introduced where each hidden unit employs a polynomial function for its activation function that is different from other units. Specifically, both a structure level as well as a function level adaptation methodologies are utilized in constructing the network. The functional level adaptation scheme ensures that the "growing" or constructive network has different activation functions for each neuron such that the network may be able to capture the underlying input-output map more effectively. The activation functions considered consist of orthonormal Hermite polynomials. It is shown through extensive simulations that the proposed network yields improved performance when compared to networks having identical sigmoidal activation functions.
Development and Application of the Multiwell Productivity Index (MPI) (2000)

Summary In this article we generalize the concept of the pseudosteady-state productivity index for the case of multiple wells producing from or injecting into a closed rectangular reservoir of constant thickness. The work complements the analytical study by Rodríguez and Cinco-Ley1 for systems produced at constant flowing pressures. Wells are represented by fully penetrating vertical line sources located arbitrarily in a homogeneous and isotropic reservoir. The multiwell productivity index (MPI) is a square matrix of dimension n, where n is the number of wells. The MPI provides a simple, reasonably accurate and fast analytical tool to evaluate well performance without dividing the cluster into single-well drainage areas. The MPI approach is used to obtain approximate analytical solutions for constant (but possibly different) wellbore flowing pressures, and to visualize the resulting pressure field. In addition, the skin factor trace technique is introduced as a tool to monitor a cluster of wells. The MPI technique is illustrated using a synthetic example taken from Ref. 2, as well as two field cases.
The Spectra and Structure of Simple Free Radicals (1972)

It is a very great pleasure to welcome another book by Dr Herzberg on to the market. On the evidence of his previous books one can be confident in expecting comprehensive coverage, a high standard of accuracy and, above all, lucidity.
Effect of Inoculum Rate on Competitive Nodulation of <i>Glycine max</i> L. Merrill. II. Field Studies<sup>1</sup> (1974)

Abstract To provide a scientific basis for predicting successful competitive nodulation of soybeans ( Glycine max L. Merr.) by Rhizobium japonicum , the quantitative relationship between soil and inoculum rhizobia, in nodule formation, was determined in the field. Soybean seeds were planted at a rate of 1 seed/2.5 cm of row and were inoculated with up to 3.3 ✕ 10 3 8 R. japonicum cells/2.5 cm of row. Field plots were grown on six soils in Iowa having a range of soil rhizobia populations of 1.2 ✕ 10 1 to 2.3 ✕ 10 5 /g of soil. Numbers of nodules on tap and lateral roots of soybeans grown on soils containing fewer than 12 rhizobia/g were increased when inoculation rates exceeded 3.3 ✕ 10 4 cells/seed. Neither numbers of nodules nor total nodule mass was increased by inoculation of soybeans grown on soils containing more than 1 ✕ 10 3 rhizobia/g. When an inoculation rate of 3.3 ✕ 10 6 rhizobia/seed was used on soybeans grown in soils with fewer than 12 rhizobia/g and in soils with approximately 1 ✕ 10 3 rhizobia/g, the inoculum strain produced, respectively, 65% and 35% of the nodules. From our results it may be predicted that if the inoculum rhizobia are to form 50% or more of the nodules an inoculum rate of at least 1,000 times the soil population (per g soil) must be used. Commercial inoculants in the United States probably are not supplying adequate numbers of rhizobia for successful competitive nodulation of soybeans grown on land previously cropped to soybeans.
Structural basis of YAP recognition by TEAD4 in the Hippo pathway (2010)

The Hippo signaling pathway controls cell growth, proliferation, and apoptosis by regulating the expression of target genes that execute these processes. Acting downstream from this pathway is the YAP transcriptional coactivator, whose biological function is mediated by the conserved TEAD family transcription factors. The interaction of YAP with TEADs is critical to regulate Hippo pathway-responsive genes. Here, we describe the crystal structure of the YAP-interacting C-terminal domain of TEAD4 in complex with the TEAD-interacting N-terminal domain of YAP. The structure reveals that the N-terminal region of YAP is folded into two short helices with an extended loop containing the PXXPhiP motif in between, while the C-terminal domain of TEAD4 has an immunoglobulin-like fold. YAP interacts with TEAD4 mainly through the two short helices. Point mutations of TEAD4 indicate that the residues important for YAP interaction are required for its transforming activity. Mutagenesis reveals that the PXXPhiP motif of YAP, although making few contacts with TEAD4, is important for TEAD4 interaction as well as for the transforming activity.
Inclusion of nanosized silicon grains in hydrogenated protocrystalline silicon multilayers and its relation to stability (2006)

Photoluminescence and Fourier transform infrared spectroscopy measured at room temperature produce strong evidence that nanosized silicon (nc-Si) grains embedded in hydrogenated protocrystalline silicon (i-pc-Si:H) multilayers. Thus, we propose the structure of the i-pc-Si:H multilayer possessing isolated nc-Si grains and their wrapping layers with a high hydrogen concentration embedded in highly hydrogen-diluted sublayers. The isolated nc-Si grains may act as radiative recombination centers of photoexcited carriers, and hence suppress the photocreation of dangling bonds caused by the nonradiative recombination in amorphous silicon matrix. Because of the repeatedly layered structure, the i-pc-Si:H multilayers have a fast light-induced metastability with a low degradation.
CVnCoV protects human ACE2 transgenic mice from ancestral B BavPat1 and emerging B.1.351 SARS-CoV-2 (2021)

Abstract The ongoing severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic necessitates the fast development of vaccines as the primary control option. Recently, viral mutants termed “variants of concern” (VOC) have emerged with the potential to escape host immunity. VOC B.1.351 was first discovered in South Africa in late 2020, and causes global concern due to poor neutralization with propensity to evade preexisting immunity from ancestral strains. We tested the efficacy of a spike encoding mRNA vaccine (CVnCoV) against the ancestral strain BavPat1 and the novel VOC B.1.351 in a K18-hACE2 transgenic mouse model. Naive mice and mice immunized with formalin-inactivated SARS-CoV-2 preparation were used as controls. mRNA-immunized mice developed elevated SARS-CoV-2 RBD-specific antibody as well as neutralization titers against the ancestral strain BavPat1. Neutralization titers against VOC B.1.351 were readily detectable but significantly reduced compared to BavPat1. VOC B.1.351-infected control animals experienced a delayed course of disease, yet nearly all SARS-CoV-2 challenged naïve mice succumbed with virus dissemination and high viral loads. CVnCoV vaccine completely protected the animals from disease and mortality caused by either viral strain. Moreover, SARS-CoV-2 was not detected in oral swabs, lung, or brain in these groups. Only partial protection was observed in mice receiving the formalin-inactivated virus preparation. Despite lower neutralizing antibody titers compared to the ancestral strain BavPat1, CVnCoV shows complete disease protection against the novel VOC B.1.351 in our studies.
Substitution Reactions of Dehydroabietic Acid. II (1941)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSubstitution Reactions of Dehydroabietic Acid. IIWilliam P. Campbell and Morley MorganaCite this: J. Am. Chem. Soc. 1941, 63, 7, 1838–1843Publication Date (Print):July 1, 1941Publication History Published online1 May 2002Published inissue 1 July 1941https://doi.org/10.1021/ja01852a012RIGHTS & PERMISSIONSArticle Views110Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (644 KB) Get e-Alerts Get e-Alerts
Accounting for memory bank contention and delay in high-bandwidth multiprocessors (1995)

This paper considers issues of memory performance in shared memory multiprocessors that provide a high-bandwidth network and in which the memory banks are slower than the processors.
The Arsenic Hyperaccumulator Fern<i>Pteris vittata</i>L. (2009)

Arsenic (As) contaminated soils and waters are becoming major global environmental and human health risks. The identification of natural hyperaccumulators of As opens the door for phytoremediation of the arsenic contaminant. Pteris vittata is the first identified naturally evolving As hyperaccumulator. More than a decade after its discovery, we have made great progress in understanding the uptake, transport, and detoxification of As in the fern. The molecular mechanisms controlling As accumulation in P. vittata are now beginning to be recognized. In this review, we will try to summarize what we have learned about this As accumulator, with particular emphasis on the current knowledge of the physiological and molecular mechanisms of arsenic phytoremediation. We also discuss the potential strategies to further enhance phytoextraction abilities of P. vittata.
The amino acid sequences of human and pig <scp>l</scp>‐arginine:glycine amidinotransferase (1994)

We have isolated and sequenced the L-arginine:glycine amidinotransferase of pig kidney mitochondria. Due to endogenous proteolysis, the purified molecules showed some heterogeneity at the N terminus. The longest form recovered had 386 amino acids. Part of the pig amidinotransferase sequence information was used to isolate cDNA clones coding for the human enzyme. The deduced amino acid of the human amidinotransferase was 37 amino acids longer due to the presence of a single sequence. The mature proteins were 94% identical to each other and 36% identical to the sequences of bacterial L-arginine:inosamine phosphate amidinotransferases.