gebhart/patrepeval huggingface dataset:
Human β1,4 galactosyltransferase and α2,6 sialyltransferase expressed in <i>Saccharomyces cerevisiae</i> are retained as active enzymes in the endoplasmic reticulum (1994)

Biosynthesis and intracellular transport of recombinant human full-length beta 1,4 galactosyltransferase (GT) and full-length alpha 2,6 sialyltransferase (ST) were investigated in Saccharomyces cerevisiae. Recently, enzymic activity of recombinant GT (rGT) in crude homogenates of S. cerevisiae could successfully be demonstrated [Krezdorn, C., Watzele, G., Kleene, R. B., Ivanov, S. X. & Berger, E. G. (1993) Eur. J. Biochem. 212, 113-120]. In the present work, we show that, in yeast strains transformed with plasmid pDPSIA containing the cDNA coding for human ST, rST enzymic activity using asialo-fetuin or N-acetyllactosamine as acceptor substrates could readily be detected. Analysis by 1H-NMR spectroscopy of the disaccharide product of rGT, as recently reported, and the trisaccharide product of rST demonstrated that only the expected glycosidic linkages were formed. Following mechanical disruption of yeast cells, both enzymes sedimented with a fraction enriched in membranes of the endoplasmic reticulum (ER) and were activated by Triton X-100 3-5-fold. rGT and rST could be immunoprecipitated from their [35S]Met-labelled transformed yeast extracts using polyclonal antibodies raised against fusion proteins consisting of beta-galactosidase-GT or beta-galactosidase-ST, respectively, expressed in Escherichia coli. For rGT a single glycosylated form of apparent molecular mass 48 kDa was reported, but for rST two main bands corresponding to apparent molecular masses of 48 kDa and 44 kDa, respectively, were detected. Immunoprecipitation from either tunicamycin-treated [35S]Met-labelled transformed yeast cells or labelling with radio-active sugars both indicated that the 44-kDa form of rST was non-glycosylated and that the 48-kDa form of rST was core N-glycosylated. In addition, core glycosylation of both recombinant enzymes demonstrated that they were competent for translocation across the ER membranes. However, the 44-kDa form of rST was converted to the 48-kDa glycosylated form only slowly, suggesting a mechanism of posttranslational translocation. Absence of hyperglycosylation of rST and rGT in wild type and lack of the Golgi-specific man-alpha 1,6-man epitope suggest that the recombinant enzymes did not enter the yeast Golgi apparatus. These results indicated that both rGT and rST are retained as enzymically active enzymes in the ER of yeast and suggest a ribonucleoprotein-independent import of rST into the ER.
Lipid microspheres for drug delivery from the pharmaceutical viewpoint. (1994)

Lipid microsphere (LM) preparations of corticosteroid, nonsteroid, anti-inflammatory drugs and prostaglandins all showed more potent activity than free drugs. The LM, with a diameter of 0.2 microns, were drug carriers prepared from soybean oil and lecithin, with the drug being incorporated within the LM. This study suggests that the LM is a very promising carrier in a drug delivery system. Future trends in this field are outlined, followed by a discussion of physicochemical properties in the evaluation of novel emulsion delivery systems.
Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. (1975)

We described previously a simple test on petri plates for detecting chemical carcinogens as mutagens, using an especially sensitive set of bacterial strains to detect mutagenic acitivty and a mammalian liver extract for carcinogen activity. We now extend the utility of the method by introducing two new bacterial strains which can detect with great sensitivity many carcinogens which we did not detect before or detected with less sensitivity. Among these carcinogens are aflatoxin B-1, sterigmatocystin, benzyl chloride, benzo[a]-pyrene, 7,12-dimethylbenzanthracene, 1'-acetoxysafrole, and the nitrofuran food additive furylfuramide (AF-2). The new strains TA100 and TA98 contain an R factor plasmid, pKM101, in our standard tester strains TA1535 and TA1538. The R factor increases mutagenesis with certain mutagens, but not others. We present evidence that the mutagens that become more effective work through an error-prone recombinational repair.
Reducing Ammonia Loss from Urea by Mixing with Humic and Fulvic Acids Isolated from Coal (2009)

Problem statement: Ammonia volatilization is a major pathway for nitrogen loss from surface applied urea. While all top-dressed ammonia and ammonium based N fertilizers can volatilize, the potential loss is greatest with urea and fluids containing urea. As much as 20-50% of N applied to soils is lost through volatilization alone. Thus, the objective of this laboratory study was to reduce ammonia loss from urea via mixing with humic and fulvic acids isolated from coal. Approach: This study compared four different types of treatments which were urea without additives (T1), urea with humic acid-powdered form (T2), urea with fulvic acid-liquid form (T3) and urea with humic and fulvic acids-liquid form (T4). Comparisons were made based on ammonia loss, soil NH4 and NO3- contents as well as exchangeable cations in the treated soils. Soil samples from typic paleudults (Bekenu series) were used. Humic substances were isolated using standard procedures. Daily ammonia loss from soil was measured using a modified closed-dynamic air flow system method. Results: All of the treatments with humic substances significantly reduced ammonia loss ranging between 13 and 25% compared to urea alone. The treatment with both humic and fulvic acids (T4) showed pronounced ammonia loss reduction. All treatments with humic substances significantly increased NH4+ and NO3- content in soil samples compared to urea alone except for treatment having humic acid alone (T2). Treatments with fulvic acid (T3 and T4) also showed significant increase in exchangeable K+ and Na+ compared to urea alone. The increase in the formation of NH4+ over NH3, soil exchangeable cations and temporary reduction of soil pH may had retarded urea hydrolysis in the immediate vicinity of the fertilizer. Conclusion: Surface applied urea fertilizer efficiency could be increased if applied together with humic and fulvic acids.
Center wordline cell: A new symmetric layout cell for 64 Mb SRAM (2002)

A new symmetric memory cell, in which one wordline is placed at the center, has been developed for 64 Mb SRAM. This new center wordline cell has the benefits of a small cell size, good stability with operation voltages as low as 1.7 V, and suitability for implementation of phase shift lithography. A high performance TFT, which has on/off ratio of 7 orders even at 2.5 V operation, is mounted in this cell.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
ELECTROCHEMICAL PREPARATION OF ERYTHROSIN AND EOSIN (1987)

The paper highlights the preparation of erythrosin (tetra iodofluorescein) and eosin (tetra bromofluorescein) by electrolytic method at a graphite anode using a solution of sodium carbonate containing iodine and fluorescein for erythrosin and sodium bromide and fluorescein for eosin at a divided cell. The yield is compared with that of chemical method.
The influence of clinical information on the reporting of CT by radiologists. (2000)

The aim of the study was to determine whether clinical information alters the CT report. This prospective blinded study consisted of 50 consecutive patients who attended a Department of Radiology for CT. Each study was interpreted by two of three consultant radiologists, before and after knowledge of the clinical information. 19 reports were changed after clinical information was known. Clinical follow-up was available in 15 cases. In ten cases the reports were more accurate after clinical information and in five cases the reports were less accurate. In three of the five cases where accuracy was reduced, the clinical information was incorrect. It was concluded that clinical information affects the CT report. If the information is accurate it has a beneficial effect; if it is inaccurate it has a detrimental effect. The more complex the investigation, the more important the clinical information. There was a correlation between readers regarding the influence of clinical information. Correct clinical information therefore improves the radiology report. It is the responsibility of the clinician to provide this information in an accurate and legible form.
Powering Virtual Try-On via Auxiliary Human Segmentation Learning (2019)

Image-based virtual try-on for fashion has gained considerable attention recently. This task requires to fit an in-shop cloth image on a target model image. An efficient framework for this is composed of two stages: (1) warping the try-on cloth to align with the body shape and pose of the target model, and (2) an image composition module to seamlessly integrate the warped try-on cloth onto the target model image. Existing methods suffer from artifacts and distortions in their try-on output. In this work, we propose to use auxiliary learning to power an existing state-of-the-art virtual try-on network. We leverage prediction of human semantic segmentation (of the target model wearing the try-on cloth) as an auxiliary task and show that it allows the network to better model the bounds of the clothing item and human skin, thereby producing a better fit. Using exhaustive qualitative and quantitative evaluation we show that there is a significant improvement in the preservation of characteristics of the cloth and person in the final try-on result, thereby outperforming the existing state-of-the-art virtual try-on framework.
High-Viscosity HPMC as a Film-Coating Agent (1993)

The possibility of using high viscosity grades of hydroxypropylmethylcellulose (HPMC) as a film forming agent in a conventional coating process has been investigated. Ethanol/water mixtures having different weight ratios (5 to 18) and containing up to 5 % of HPMC revealed to be suitable.The influence of some formulation additives (talc, PVP and plasticizers) on both the overall coating process and the in-vitro release profiles of ketoprofen containing coated tablets was also investigated and discussed.
Abbe's Sine Condition (1998)

Get PDF Email Share Share with Facebook Tweet This Post on reddit Share with LinkedIn Add to CiteULike Add to Mendeley Add to BibSonomy Get Citation Copy Citation Text MASUD MANSURIPUR, "Abbe's Sine Condition," Optics & Photonics News 9(2), 56-60 (1998) Export Citation BibTex Endnote (RIS) HTML Plain Text Citation alert Save article
Scalable data clustering using GPUs (2010)

The computational demands of multivariate clustering grow rapidly, and therefore processing large data sets, like those found in flow cytometry data, is very time consuming on a single CPU. Fortunately these techniques lend themselves naturally to large scale parallel processing. To address the computational demands, graphics processing units, specifically NVIDIA's CUDA framework and Tesla architecture, were investigated as a low-cost, high performance solution to a number of clustering algorithms. C-means and Expectation Maximization with Gaussian mixture models were implemented using the CUDA framework. The algorithm implementations use a hybrid of CUDA, OpenMP, and MPI to scale to many GPUs on multiple nodes in a high performance computing environment. This framework is envisioned as part of a larger cloud-based workflow service where biologists can apply multiple algorithms and parameter sweeps to their data sets and quickly receive a thorough set of results that can be further analyzed by experts. \t Improvements over previous GPU-accelerated implementations range from 1.42x to 21x for C-means and 3.72x to 5.65x for the Gaussian mixture model on non-trivial data sets. Using a single NVIDIA GTX 260 speedups are on average 90x for C-means and 74x for Gaussians with flow cytometry files compared to optimized C code running on a single core of a modern Intel CPU. Using the TeraGrid Lincoln high performance cluster at NCSA C-means achieves 42% parallel efficiency and a CPU speedup of 4794x with 128 Tesla C1060 GPUs. The Gaussian mixture model achieves 72% parallel efficiency and a CPU speedup of 6286x.
Technetium-99m-tetrofosmin: retention of nitrogen atmosphere in kit vial as a cause of poor quality material (2000)

Technetium-99m (99Tc(m))-tetrofosmin was prepared using four different reconstitution methods. The radiochemical purity (RCP) of these products was assessed 8 h later using thin layer chromatography (TLC). Material produced using the original method supplied by the manufacturer and using an newer method, which involves the use of a vent needle and the addition of air, had acceptable RCP (mean +/- SD 94.2 +/- 1.1% and 94.7 +/- 1.7%, respectively) and similar chromatograms. In addition, both products showed good clinical efficacy and exhibited normal biodistribution behaviour. Preparing 99Tc(m)-tetrofosmin using the two other methods, one using a high radioactive concentration and the other maintaining the nitrogen content of the kit vial, gave rise to chromatograms with reduced RCP (63.5 +/- 10.9% and 61.9 +/- 7.6%, respectively) and greater levels of impurities. Although neither of these last two preparations was used clinically, we suggest that reports of poor quality images may be the result of administration of materials similar to these. Results for the high radioactive concentration method were as expected and are consistent with the restrictions imposed by the manufacturer. However, results using the last method are surprising and would suggest that the production of good quality 99Tc(m)-tetrofosmin is dependent on the quantity of nitrogen in the kit vial. We believe that the amount of nitrogen removed from the kit vial during the process of reconstitution is critical. If too much nitrogen is present this will result in poor quality material. In practice it is conceivable that there could be occasions when insufficient nitrogen is removed when following the manufacturer's original guidance, thereby leading to low RCP material. To ensure adequate nitrogen is removed during reconstitution, adoption of the manufacturer's revised method, involving the deliberate introduction of air, is therefore appropriate.
10-Gb/s high-speed monolithically integrated photoreceiver using InGaAs p-i-n PD and planar doped InAlAs/InGaAs HEMTs (1992)

A long-wavelength monolithically integrated photoreceiver which is capable of operation at a 10-Gb/s NRZ light signal is described. The photoreceiver was fabricated using an Si-planar-doping technique to enhance the uniformity and transconductance of HEMTs and using Be-ion implantation technique. The circuit consists of a p-i-n PD, a transimpedance amplifier, and a buffer stage to provide an output impedance of 50 Omega . The photoreceiver demonstrated a 3-dB down bandwidth of 8 GHz and the responsivity of 158 V/W. The measured input noise current was 20 pA/ square root Hz and the calculated sensitivity at 10-Gb/s NRZ signal was -16.5 dBm. The receiver is the first monolithically integrated p-i-n-HEMT receiver capable of receiving a 10-Gb/s NRZ light signal.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Further descriptions of new birds from El Salvador (1928)

(Uploaded by Plazi from the Biodiversity Heritage Library) No abstract provided.
The effect of oral N-acetylcysteine on lung glutathione levels in idiopathic pulmonary fibrosis (1994)

Idiopathic pulmonary fibrosis (IPF) is characterized by an increased oxidant burden and by a deficiency of glutathione, a major antioxidant, in the lung epithelial lining fluid (ELF). Therefore, a rational therapeutic approach is to reverse the imbalance between oxidants and antioxidants in the lung by enhancing the antioxidant screen. With this background, the aim of our study was to evaluate oral N-acetylcysteine (NAC) as a strategy to augment lung glutathione levels in patients with IPF. Concentrations of total glutathione in bronchoalveolar lavage fluid (BALF) were quantified spectrophotometrically, before and following oral therapy with 3 x 600 mg NAC per day for 5 days, in 17 nonsmoking patients with biopsy-proven IPF. The volume of ELF recovered by BAL was determined using the urea method. Pretherapy, total glutathione levels in ELF in IPF patients were significantly less than normal (187 +/- 36 vs 368 +/- 60 microM), in contrast to levels in BALF (0.99 +/- 0.12 vs 1.18 +/- 0.19 microM). Following therapy with oral NAC, glutathione levels in BALF were 1.54 +/- 0.24 microM (a significant increase compared to pretherapy), whereas the increase in ELF levels (319 +/- 92 microM) did not reach significance. The therapy was well-tolerated, and all routine clinical and bronchoscopic parameters remained unchanged. It is thus feasible and safe to augment deficient lung glutathione levels in patients with IPF; thereby, potentially augmenting pulmonary antioxidant protection.
Responsiveness of isolated adrenocortical cells from lean and obese Zucker rats to ACTH (1988)

Morning plasma corticosterone concentrations have been reported to be elevated in obese Zucker rats compared with lean rats. The aim of this study was to determine if differences in adrenal sensitivity or maximal responsiveness to adrenocorticotropic hormone (ACTH)-(1-24) could account for this disparity. Serum and adrenal glands were collected from lean and obese, male and female Zucker rats (10-13 wk old) between 10:00 and 11:00 A.M. Adrenocortical cells were isolated and challenged with ACTH-(1-24). The serum corticosterone and ACTH concentrations were significantly greater in obese males compared with lean males (45.3 +/- 10.3 vs. 23.2 +/- 1.45 ng/ml and 156.6 +/- 15.3 vs. 113.3 +/- 9.4 pg/ml, respectively). Although serum corticosterone concentrations were similar in female rats, serum ACTH concentrations tended (P = 0.07) to be lower in obese female rats than in lean female rats (67.6 +/- 9.3 vs. 103.5 +/- 15.1 pg/ml, respectively). The median effective concentration (EC50) and the maximal corticosterone response per microgram of DNA of dose-response curves derived from lean and obese rats were not significantly different. Additionally, a morphometric evaluation of adrenal tissue from lean and obese rats suggested that cells of the zona glomerulosa were smaller in obese rats than in lean rats. Our data confirm that morning serum corticosterone concentrations are elevated in 10- to 13-wk-old male Zucker rats. This difference does not appear to be due to differences in the sensitivity or maximal secreting capacity of adrenocortical cells to ACTH.
Role of SERPINB3 in hepatocellular carcinoma (2014)

SERPINB3 (formerly known as squamous cell carcinoma antigen-1 or SCCA1) is a member of the family of serine-protease inhibitors. SERPINB3 protects cells from oxidative stress conditions, but in chronic liver damage this serpin may lead to hepatocellular carcinoma through different strategies, including inhibition of apoptosis, induction of epithelial to mesenchymal transition and decrease of desmosomal junctions, cell proliferation and invasiveness. SERPINB3 may also contribute to tumor cell resistance to anti-neoplastic drugs through its binding to the respiratory Complex I, protecting cells from the pro-oxidant action of chemotherapeutic agents. Mechanisms of tumor growth promotion induced by SERPINB3 include the inhibition of intratumor infiltration of natural killer cells, up-regulation of Myc oncogene and the recent identification of this serpin as a Ras-responsive factor. In the liver SERPINB3 and SERPINBB4 isoforms (known as squamous cell carcinoma antigen or SCCA) are undetectable in normal hepatocytes, but their expression progressively increases in chronic liver diseases, dysplastic nodules and hepatocellular carcinoma. High SERPINB3 levels have been recently detected in HCC tissue of patients with early tumor recurrence after surgical resection. In serum SERPINB3/4 isoforms (or SCCA) are detectable bound to IgMs (SCCA-IgM) in the majority of HCV infected patients with HCC and in patients with cirrhosis their levels and/or the progressive increase have been found correlated to the risk of HCC development. Preliminary findings in patients with HCC revealed that SCCA-IgM was predictive of HCC prognosis, since low levels of this biomarker were able to identify HCC patients with long overall and progression-free survival.
Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody‐coated gold nanoparticles as nanoprobes (2013)

A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N = 3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N = 3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n = 11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results.
Maillard Reactions in Chemistry, Food and Health (1994)

Oral Presentations: Plenary Lectures Chemistry Food Health: Poster Presentations - Abstracts: Chemistry Pharmacology/Biochemistry Toxicology/Aging Subject Index.
α<sub>1B</sub> N‐Type Calcium Channel Isoforms with Distinct Biophysical Properties (1999)

N-type calcium channels both generate the initial calcium signal to trigger neurotransmitter release and also interact with synaptic release proteins at many mammalian central nervous system synapses. Two isoforms of the alpha 1B N-type channel from rat brain (alpha 1B-I and alpha 1B-II) were found to differ in four regions: (1) a glutamate (Glu) to glycine (Gly) substitution in domain I S3; (2) a Gly to Glu substitution in the domain I-II linker; (3) the insertion or deletion of an alanine (Ala) in the domain I-II linker; and (4) the presence or absence of serine/phenylalanine/methionine/glycine (SFMG) in the linker between domain III S3-S4. Comparison of the electrophysiological properties of the alpha 1B-I and alpha 1B-II N-type channels shows that they exhibit distinct kinetics as well as altered current-voltage relations. Utilizing chimeric alpha 1B-I and alpha 1B-II cDNAs, we show that: (1) the Glu 177 to Gly substitution in domain I S3 increases the rate of activation by approximately 15-fold; (2) the presence or absence of Ala 415 in the domain I-II linker alters current-voltage relations by approximately 10 mV but does not affect channel kinetics; (3) the substitution of Gly 387 to Glu in the domain I-II linker also has no effect on kinetics; and (4) the presence or absence of SFMG (1236-1239) in domain III S3-S4 did not significantly affect channel current-voltage relations, kinetics, or steady state inactivation. We conclude that molecularly distinct alpha 1B isoforms are expressed in rat brain and may account for some of the functional diversity of N-type currents in native cells.