gebhart/patrepeval huggingface dataset:
Online Hydrophobic Interaction Chromatography–Mass Spectrometry for Top-Down Proteomics (2016)

Recent progress in top-down proteomics has led to a demand for mass spectrometry (MS)-compatible chromatography techniques to separate intact proteins using volatile mobile phases. Conventional hydrophobic interaction chromatography (HIC) provides high-resolution separation of proteins under nondenaturing conditions but requires high concentrations of nonvolatile salts. Herein, we introduce a series of more-hydrophobic HIC materials that can retain proteins using MS-compatible concentrations of ammonium acetate. The new HIC materials appear to function as a hybrid form of conventional HIC and reverse phase chromatography. The function of the salt seems to be preserving protein structure rather than promoting retention. Online HIC-MS is feasible for both qualitative and quantitative analysis. This is demonstrated with standard proteins and a complex cell lysate. The mass spectra of proteins from the online HIC-MS exhibit low charge-state distributions, consistent with those commonly observed in native MS. Furthermore, HIC-MS can chromatographically separate proteoforms differing by minor modifications. Hence, this new HIC-MS combination is promising for top-down proteomics.
Endoplasmic reticulum-tethered transcription factor cAMP responsive element-binding protein, hepatocyte specific, regulates hepatic lipogenesis, fatty acid oxidation, and lipolysis upon metabolic stress in mice (2011)

All together, our study shows that CREBH plays a key role in maintaining lipid homeostasis by regulating the expression of the genes involved in hepatic lipogenesis, FA oxidation, and lipolysis under metabolic stress. The identification of CREBH as a stress-inducible metabolic regulator has important implications in the understanding and treatment of metabolic disease.
Sonochemical and electrochemical synthesis of tetramesityldisilene (1982)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSonochemical and electrochemical synthesis of tetramesityldisilenePhilip Boudjouk, Byung Hee Han, and Kevin R. AndersonCite this: J. Am. Chem. Soc. 1982, 104, 18, 4992–4993Publication Date (Print):September 1, 1982Publication History Published online1 May 2002Published inissue 1 September 1982https://pubs.acs.org/doi/10.1021/ja00382a062https://doi.org/10.1021/ja00382a062research-articleACS PublicationsRequest reuse permissionsArticle Views282Altmetric-Citations66LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Multi-task Prediction of Disease Onsets from Longitudinal Lab Tests (2016)

Disparate areas of machine learning have benefited from models that can take raw data with little preprocessing as input and learn rich representations of that raw data in order to perform well on a given prediction task. We evaluate this approach in healthcare by using longitudinal measurements of lab tests, one of the more raw signals of a patient's health state widely available in clinical data, to predict disease onsets. In particular, we train a Long Short-Term Memory (LSTM) recurrent neural network and two novel convolutional neural networks for multi-task prediction of disease onset for 133 conditions based on 18 common lab tests measured over time in a cohort of 298K patients derived from 8 years of administrative claims data. We compare the neural networks to a logistic regression with several hand-engineered, clinically relevant features. We find that the representation-based learning approaches significantly outperform this baseline. We believe that our work suggests a new avenue for patient risk stratification based solely on lab results.
A Java compatible virtual machine for wireless sensor nodes (2008)

The Java programming language has potentially significant advantages for wireless sensor nodes but there is currently no feature-rich, open source virtual machine available. In this paper we present Darjeeling, a system comprising offline tools and a memory efficient run-time. The offline post-compiler tool analyzes, links and consolidates Java class files into loadable modules. The runtime implements a modified Java VM that supports multithreading and is designed specifically to operate in constrained execution environments such as wireless sensor network nodes and supports inheritance, threads, garbage collection, and loadable modules. We have demonstrated Java running on AVR128 and MSP430 microcontrollers at speeds of up to 70,000 JVM instructions per second.
Silicate Nephrotoxicity in the Experimental Animal: The Missing Factor in Analgesic Nephropathy (1982)

Three silicon-containing compounds (magnesium trisilicate BP, crushed quartz and crushed Arran granite) were added to the drinking water (250 mg/l) of three groups of male guinea pigs for four months. At autopsy all animals receiving magnesium trisilicate showed a focal tubulo-interstitial nephritis mainly affecting the distal nephron. Similar but less intense lesions were encountered in two animals receiving crushed quartz. No renal lesions were found in the control group or in animals receiving crushed Arran granite. The concentration of soluble silicates in drinking water, measured as elemental silicon (Si) by atomic absorption spectroscopy (AAS) was determined for twenty-eight locations in different regions of Britain. Following ingestion of magnesium trisilicate, significant increases in urinary excretion of Si were demonstrated in two healthy adults using atomic absorption spectroscopy.
Efficiency Enhancement of a Piezoelectric Energy Harvesting Device in Pulsed Operation by Synchronous Charge Inversion (2005)

This paper presents a new application of the ‘Synchronized Switch Harvesting on Inductor’ (SSHI). This nonlinear technique results in a significant increase of the electromechanical conversion capability of piezoelectric materials. Previous studies have shown the interest of this technique on steady state excited structures equipped with piezoelectric elements where the harvested power may be increased nearly tenfold compared to the standard technique. It is herein demonstrated that this technique is also very effective for pulsed excitation. It is shown that the SSHI technique increases the harvesting process efficiency between 250 and 450%, depending on the electromechanical coupling coefficient of the structure.
Red emissive AIE nanodots with high two-photon absorption efficiency at 1040 nm for deep-tissue in vivo imaging (2015)

Deep-tissue penetration is highly required in in vivo optical bioimaging. We synthesized a type of red emissive fluorophore BT with aggregation-induced emission (AIE) property. BT molecules were then encapsulated with amphiphilic polymers to form nanodots, and a large two-photon absorption (2PA) cross-section of 2.9 × 10(6) GM at 1040 nm was observed from each BT nanodot, which was much larger than those at the wavelengths of 770 to 860 nm. In addition, 1040 nm light was found to have better penetration and focusing capability than 800 nm light in biological tissue, according to the Monte Carlo simulation. The toxicity and tissue distribution of BT nanodots were studied, and they were found to have good biocompatibility. BT nanodots were then utilized for in vivo imaging of mouse ear and brain, and an imaging depth of 700 μm was obtained with the femtosecond (fs) excitation of 1040 nm. The red emissive AIE nanodots with high 2PA efficiency at 1040 nm would be useful for deep-tissue functional bioimaging in the future.
The use of APACHE II scores to select candidates for left ventricular assist device placement. Acute Physiology and Chronic Health Evaluation. (1998)

LVAD placement for severe heart failure (not restricted to cardiogenic shock) improves survival. APACHE II can aid in deciding the timing of LVAD placement in patients with heart failure who may not have attained conventional hemodynamic criteria for LVAD placement. Patients who had APACHE II scores between 11 and 20 derived the greatest benefit from LVAD placement.
Surface free technology by laser annealing (SUFTLA) (2003)

A new technology, which enables the transfer of thin films or thin film devices from original substrate to any substrate by using laser annealing, has been investigated. This technology was named SUFTLA which stands for surface free technology by laser annealing. Low temperature (/spl les/425/spl deg/C) polycrystalline-silicon thin film transistors (poly-Si TFTs) and TFT circuits could be successfully transferred from glass or quartz substrate to plastic film. The circuit functionalities of CMOS ring oscillators were not affected by this transfer.
Toward Vibrational Dynamics at Liquid–Liquid and Nano-Interfaces: Time-Resolved Sum-Frequency Scattering (2014)

Interfacial molecular dynamics are key to understanding many phenomena in technology and life sciences. We demonstrate a first step toward accessing vibrational dynamics at liquid-liquid and nanoscopic (bio)-interfaces using time-resolved sum-frequency scattering to probe the interfaces of a nanodroplet platform. The free induction decay of the vibrational modes of dodecylsulfate amphiphiles from the interface of dispersed nanoscopic oil droplets was measured. We probed the vibrational response of both the functional headgroup and the alkyl tail of the amphiphiles. In the molecular fingerprint region, a beating of vibrational modes was observed. Simultaneous modeling of the time- and frequency-resolved response revealed modes at ∼995 and ∼1065 cm(-1) that can be assigned to C-O-S and SO3 stretch vibrations.
Time-Dependent Superconductivity and Quantum Dissipation (1972)

A time-dependent Ginzburg-Landau theory is used to calculate the time-varying currents and potentials in a one-dimensional superconductor undergoing quasicontinuous phase slip. To account for the irreversible nature of this process, the concept of superconducting dissipation is introduced. This analysis is consistent with many observations on "weak links" and provides an adequate model for at least their low-frequency behavior.
The BioWatch Program: Detection of Bioterrorism (2003)

This report discusses some aspects of the BioWatch Program which may be of particular interest to policymakers. For example, Congress may be interested in whether these types of detection systems can substitute for or supplement other mechanisms in protecting the general populace; whether this detection system was implemented optimally; how the success of this system is to be evaluated; whether the implementation, operational, and expansion costs for the BioWatch Program make it a cost-effective federal investment; and how to optimize and streamline performance in the future. Since the BioWatch Program is a federal program implemented using state infrastructure, Congress may wish to examine how this new program coordinates with already existing public health and counterterrorism programs, as well as consider the roles and responsibilities of the federal government and coordination with state governments in an actual bioterrorism event.
Introduction of correlative light and airSEMTM microscopy imaging for tissue research under ambient conditions (2014)

A complete fingerprint of a tissue sample requires a detailed description of its cellular and extracellular components while minimizing artifacts. We introduce the application of a novel scanning electron microscope (airSEM™) in conjunction with light microscopy for functional analysis of tissue preparations at nanometric resolution (<10 nm) and under ambient conditions. Our metal-staining protocols enable easy and detailed visualization of tissues and their extracellular scaffolds. A multimodality imaging setup, featuring airSEM™ and a light microscope on the same platform, provides a convenient and easy-to-use system for obtaining structural and functional correlative data. The airSEM™ imaging station complements other existing imaging solutions and shows great potential for studies of complex biological systems.
Characterization of In Vitro DNA Binding Sites of the EUO Protein of <i>Chlamydia psittaci</i> (2000)

The EUO gene of chlamydia is highly expressed early in the developmental cycle, relative to other genes, but continues to be expressed throughout the active growth phases. The precise function of EUO protein is not known, but it binds to DNA in vitro. In this study, we developed a selection and amplification scheme for identifying chlamydial genomic fragments to which EUO preferentially binds in vitro. The scheme involved mixing recombinant EUO with a Chlamydia psittaci genomic library in a pBluescript plasmid vector in vitro, trapping EUO-bound plasmid clones on filters, and amplifying the clones in Escherichia coli. After nine rounds of enrichment, the EUO binding sites of the three most highly enriched clones were identified by DNase I footprint analysis. All three clones had multiple binding sites of various sizes with no clear distinguishing feature other than they were AT-rich and were usually not located in putative promoter regions. We used limited site-specific mutagenesis to characterize the strongest binding site of the most-highly-enriched clone, which represented about 50% of the population after nine rounds. This mutagenesis identified a core binding site of 15 nucleotides (nt) whose sequence was used to find related sequences within each of the strong binding sites in the other two clones. Using the frequency of bases at specific positions within this group of sequences as a guide, we carried out trial-and-error searching with many related sequences, eliminating those which identified nonfootprinted sites. This process led us to the consensus 15-nt sequence AHGAAAWVTYTWDAY, which, when allowing two mismatches, picked out all of the strong binding sites and no nonfootprinting sites within the three enriched clones. This sequence may be useful for predicting additional possible EUO binding sites in the chlamydial genome.
Ultra-long glass tips for atomic force microscopy (1996)

A new variety of ultra-long tips for atomic force microscopy has been fabricated using photoetchable glass. Photoetchable glass is structured by UV exposure through a quartz mask followed by a heat treatment and etch process in hydrofluoric acid. The process is based on different etching rates with a ratio of about 1:20 between unexposed and exposed parts of the glass. Tips longer than with a tip radius down to 20 nm are possible. The cantilevers are made from silicon carbide. The mechanical properties of such long tips are compared, in theory and in practice, with conventional cantilevers from silicon and silicon nitride. Their strong sensitivity to friction forces for contrast formation in atomic force microscopy is discussed.
Traumatic Migraine Versus Concussion (2013)

Trauma-triggered migraine should be considered in the differential for recurrent trauma-induced headaches. There is a lack of research evaluating efficacy and side effects of treatment of trauma-triggered migraine, and no information was found directing when an athlete can safely return to play.
A new calibration phantom for quantitative computed tomography (1987)

We report on a new calibration phantom for quantitative computed tomography which has been improved with respect to reference materials and geometrical setup. Instead of liquid calibration solutions, we use polyethylene-based water- and bone-equivalent plastics. The size of the phantom is considerably reduced by using only two samples. This design guarantees long-term stability and it offers advantages with respect to radiation geometry.
A mutant cytochrome b5 with a lengthened membrane anchor escapes from the endoplasmic reticulum and reaches the plasma membrane. (1996)

Many resident membrane proteins of the endoplasmic reticulum (ER) do not have known retrieval sequences. Among these are the so-called tail-anchored proteins, which are bound to membranes by a hydrophobic tail close to the C terminus and have most of their sequence as a cytosolically exposed N-terminal domain. Because ER tail-anchored proteins generally have short (< or = 17 residues) hydrophobic domains, we tested whether this feature is important for localization, using cytochrome b5 as a model. The hydrophobic domain of cytochrome b5 was lengthened by insertion of five amino acids (ILAAV), and the localization of the mutant was analyzed by immunofluorescence in transiently transfected mammalian cells. While the wild-type cytochrome was localized to the ER, the mutant was relocated to the surface. This relocation was not due to the specific sequence introduced, as demonstrated by the ER localization of a second mutant, in which the original length of the membrane anchor was restored, while maintaining the inserted ILAAV sequence. Experiments with brefeldin A and with cycloheximide demonstrated that the extended anchor mutant reached the plasma membrane by transport along the secretory pathway. We conclude that the short membrane anchor of cytochrome b5 is important for its ER residency, and we discuss the relevance of this finding for other ER tail-anchored proteins.
A practice-based analysis of combinations of diseases in patients aged 65 or older in primary care (2014)

This study confirms the high prevalence of multimorbidity in patients aged over 65 in primary care. Hypertension-osteoarthritis is defined as a frequent combination however 94% of these patients have more than two disorders. The low prevalence of specific combinations, the high prevalence of psychiatric and social problems and the general complexity of multimorbidity will hamper the usefulness of randomized trials or guidelines at practice level. There is a need to explore new paradigms for addressing multimorbidity.
Identification of epoxide metabolites of tamoxifen by on-line liquid chromatography-electrospray ionization mass spectrometry (1994)

Conference Article| May 01 1994 Identification of epoxide metabolites of tamoxifen by on-line liquid chromatography-electrospray ionization mass spectrometry CHANG KEE LIM; CHANG KEE LIM 1MRC Toxicology Unit, Hodgkin Building, University of. Leicester, P.O. Box 138, Lancaster Road, Leicester LE1 9HN, U.K. Search for other works by this author on: This Site PubMed Google Scholar JOHN H. LAMB; JOHN H. LAMB 1MRC Toxicology Unit, Hodgkin Building, University of. Leicester, P.O. Box 138, Lancaster Road, Leicester LE1 9HN, U.K. Search for other works by this author on: This Site PubMed Google Scholar ZHI XIN YUAN; ZHI XIN YUAN 1MRC Toxicology Unit, Hodgkin Building, University of. Leicester, P.O. Box 138, Lancaster Road, Leicester LE1 9HN, U.K. Search for other works by this author on: This Site PubMed Google Scholar LEWIS L. SMITH LEWIS L. SMITH 1MRC Toxicology Unit, Hodgkin Building, University of. Leicester, P.O. Box 138, Lancaster Road, Leicester LE1 9HN, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1994) 22 (2): 165S. https://doi.org/10.1042/bst022165s Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation CHANG KEE LIM, JOHN H. LAMB, ZHI XIN YUAN, LEWIS L. SMITH; Identification of epoxide metabolites of tamoxifen by on-line liquid chromatography-electrospray ionization mass spectrometry. Biochem Soc Trans 1 May 1994; 22 (2): 165S. doi: https://doi.org/10.1042/bst022165s Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search © 1994 Biochemical Society1994 Article PDF first page preview Close Modal You do not currently have access to this content.
Immunologic Properties of Bacterial Lipopolysaccharide (LPS): Correlation between the Mitogenic, Adjuvant, and Immunogenic Activities (1975)

Abstract Bacterial lipopolysaccharide (LPS) was demonstrated to have the capacity in mice to enhance the response to soluble bovine serum albumin (BSA) and to interfere with the induction of tolerance to human γ-globulin (HGG). These adjuvant activities were shown to occur under conditions in which LPS could also function as a B cell mitogen. This positive correlation was established by utilizing two experimental situations in which LPS was non-mitogenic for spleen cells. Thus, on the one hand, it was found that LPS did not function as an adjuvant in C3H/HeJ mice, a unique strain whose spleen cells were also unresponsive to LPS-induced mitogenesis. On the other hand, in strains which did respond to LPS mitogenically, LPS failed to function as an adjuvant when it was chemically altered to reduce its in vitro mitogenic activity. A correlation was also observed between mitogenesis and the capacity of LPS to function as a specific immunogen in mice. In contrast to the sustained and prolonged plaque-forming cell response that was observed in mice whose spleen cells were also responsive to LPS-induced mitogenesis, the response was relatively transient in the C3H/HeJ strain. These results are discussed in view of the possible in vivo modes of action of LPS.
A 150-V multiple up-drain VDMOS, CMOS, and bipolar process in 'direct-bonded' silicon on insulator on silicon (1992)

Silicon on insulator on silicon (SOIS) has been produced with silicon direct bonding (SDB). Within a silicon film of 15- mu m thickness, islands with ubiquitous oxide isolation have been formed for the simultaneous integration of 150-V power VDMOS transistors, CMOS circuits in a channelless sea-of-gates array with 2- mu m gates, and bipolar transistors. The up-drain VDMOS transistors with 2- Omega -mm/sup 2/ specific on-resistance allow multiple isolated outputs, so high-voltage push-pull drivers can be fabricated in a single chip. The bipolar transistors are comparable to those of a 60-V standard process with vertical n-p-n and lateral p-n-p current gains of 80.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Optical properties of epitaxial CoSi2/Si and CoSi2 particles in Si from 0.062 to 2.76 eV (1992)

We have measured the optical properties of epitaxial CoSi2 films on Si from 0.062 to 2.76 eV by ellipsometry and spectrophotometry. The energy dependencies of the dielectric constants show Drude behavior at energies lower than ∼0.2 eV with Drude parameters ℏωp=(5.8±0.2) eV and ℏ/τ=(0.09±0.02) eV. Using the measured optical constants, the CoSi2 film is shown to have maximum absorptance at a thickness of ∼20 nm for λ≳1.4 μm. Finally, we have calculated the absorptance of a composite film of CoSi2 particles embedded in Si and found that the absorptance peak due to a surface plasmon resonance in the CoSi2 particles shifts to higher energy as the ellipsoidal particles become more elongated, in agreement with recent observations by Fathauer et al. [Phys. Rev. B 44, 1345 (1991)].
Interactions of Calcium, pH, Temperature, and Chymosin During Milk Coagulation (1985)

Holstein milk samples with good and poor chymosin-coagulation characteristics were coagulated in the Formagraph using different combinations of five levels of chymosin, three pH, and three temperatures in the presence and absence of .02% added calcium chloride.