gebhart/patrepeval huggingface dataset:
TrkB Agonist Antibody Pretreatment Enhances Neuronal Survival and Long-Term Sensory Motor Function Following Hypoxic Ischemic Injury in Neonatal Rats (2014)

Perinatal hypoxic ischemia (H-I) causes brain damage and long-term neurological impairments, leading to motor dysfunctions and cerebral palsy. Many studies have demonstrated that the TrkB-ERK1/2 signaling pathway plays a key role in mediating the protective effect of brain-derived neurotrophic factor (BDNF) following perinatal H-I brain injury in experimental animals. In the present study, we explored the neuroprotective effects of the TrkB-specific agonist monoclonal antibody 29D7 on H-I brain injury in neonatal rats. First, we found that intracerebroventricular (icv) administration of 29D7 in normal P7 rats markedly increased the levels of phosphorylated ERK1/2 and phosphorylated AKT in neurons up to 24 h. Second, P7 rats received icv administration of 29D7 and subjected to H-I injury induced by unilateral carotid artery ligation and exposure to hypoxia (8% oxygen). We found that 29D7, to a similar extent to BDNF, significantly inhibited activation of caspase-3, a biochemical hallmark of apoptosis, following H-I injury. Third, we found that this 29D7-mediated neuroprotective action persisted at least up to 5 weeks post-H-I injury as assessed by brain tissue loss, implicating long-term neurotrophic effects rather than an acute delay of cell death. Moreover, the long-term neuroprotective effect of 29D7 was tightly correlated with sensorimotor functional recovery as assessed by a tape-removal test, while 29D7 did not significantly improve rotarod performance. Taken together, these findings demonstrate that pretreatment with the TrkB-selective agonist 29D7 significantly increases neuronal survival and behavioral recovery following neonatal hypoxic-ischemic brain injury.
Direct-Tissue SELDI-TOF Mass Spectrometry Analysis: A New Application for Clinical Proteomics (2006)

Direct tissue proteomic analysis is an original application of SELDI-TOF MS technology that can expand the use of clinical proteomics as a complement to the anatomopathological diagnosis.
High fat diet induces dysregulation of hepatic oxygen gradients and mitochondrial function<i>in vivo</i> (2008)

NAFLD (non-alcoholic fatty liver disease), associated with obesity and the cardiometabolic syndrome, is an important medical problem affecting up to 20% of western populations. Evidence indicates that mitochondrial dysfunction plays a critical role in NAFLD initiation and progression to the more serious condition of NASH (non-alcoholic steatohepatitis). Herein we hypothesize that mitochondrial defects induced by exposure to a HFD (high fat diet) contribute to a hypoxic state in liver and this is associated with increased protein modification by RNS (reactive nitrogen species). To test this concept, C57BL/6 mice were pair-fed a control diet and HFD containing 35% and 71% total calories (1 cal approximately 4.184 J) from fat respectively, for 8 or 16 weeks and liver hypoxia, mitochondrial bioenergetics, NO (nitric oxide)-dependent control of respiration, and 3-NT (3-nitrotyrosine), a marker of protein modification by RNS, were examined. Feeding a HFD for 16 weeks induced NASH-like pathology accompanied by elevated triacylglycerols, increased CYP2E1 (cytochrome P450 2E1) and iNOS (inducible nitric oxide synthase) protein, and significantly enhanced hypoxia in the pericentral region of the liver. Mitochondria from the HFD group showed increased sensitivity to NO-dependent inhibition of respiration compared with controls. In addition, accumulation of 3-NT paralleled the hypoxia gradient in vivo and 3-NT levels were increased in mitochondrial proteins. Liver mitochondria from mice fed the HFD for 16 weeks exhibited depressed state 3 respiration, uncoupled respiration, cytochrome c oxidase activity, and mitochondrial membrane potential. These findings indicate that chronic exposure to a HFD negatively affects the bioenergetics of liver mitochondria and this probably contributes to hypoxic stress and deleterious NO-dependent modification of mitochondrial proteins.
Inhibition of bradykinin- and kallikrein-induced cerebral arteriolar dilation by a specific bradykinin antagonist. (1987)

We have previously shown that topical brain application of kallikrein, an enzyme which converts kininogen to bradykinin, induces rabbit pial arteriole dilation. The purpose of the present investigation was to utilize a newly developed competitive kinin receptor antagonist to test the hypothesis that kallikrein-induced dilation was due to the conversion of brain kininogen to vasoactive kinins. As in our previous study, we measured rabbit pial arteriole diameter with a microscope using the closed cranial window technique. The kinin antagonist (6 microM) reduced the dose-dependent dilation produced by bradykinin and blocked the dilation induced by kallikrein. In addition, the kinin antagonist was specific since it did not alter the cerebral arteriole dilation produced by adenosine, acetylcholine, or vasoactive intestinal polypeptide. These experiments provide further evidence for a possible role of the endogenous brain kallikrein-kinin system in the modulation of the cerebral circulation and provide the necessary pharmacologic foundation for future use of this antagonist in testing the role of kinins in the normal or altered cerebral circulation.
A novel trajectory restoration algorithm for high-resolution SAR imaging (2014)

The quality of high-resolution SAR imaging is strictly related with the precision of the platform trajectory measurements. This is one of the crucial points in the case of image formation from light-weight aircrafts and UAV platforms, which undergone significant trajectory instabilities in real flight conditions. In the paper, a new approach for the residual range-dependent phase error restoration is proposed. According to the method, the cross-track aircraft accelerations are evaluated via a specifically developed weighted estimation procedure. The reconstructed residual trajectory deviations are used for the range-dependent motion errors compensation. The corresponding experimental results are also demonstrated.
Different roles of interleukin 6 and interleukin 11 in the liver: implications for therapy (2020)

The interleukin 6 (IL6) family of proteins regulate important cellular processes and act through a variety of signaling pathways via a shared gp130 receptor. In the liver, there is a large body of evidence showing a protective and pro-regenerative role for IL6 <i>cis</i> and <i>trans</i> signaling. While a few studies suggest a pathological role for IL6 <i>trans</i>-signaling in the liver. IL11 is often thought of as similar to IL6 and redundancy has been inferred. However, recent studies reveal that IL6R and IL11RA are expressed on dissimilar cell types and these cytokines actually have very different roles in biology and pathology. In the liver, IL6R is mostly expressed on immune cells, whereas IL11RA is highly expressed on hepatocytes and hepatic stellate cells, both of which exhibit autocrine IL11 activity. In contrast to the beneficial effects of IL6 in the liver, IL11 causes liver disease and its expression in stromal and parenchymal cells leads to fibrosis, inflammation, steatosis and hepatic failure. In this review, we address IL6 and IL11 in the context of liver function. We end by discussing the possibility of IL6 gain-of-function versus IL11 inhibition as therapeutic approaches to treat liver disease. <sup>1,2</sup>.
Broadband 90$^{\circ}$ Differential Phase Shifter Constructed Using a Pair of Multisection Radial Line Stubs (2012)

The current paper proposes a broadband 90 <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">°</sup> differential phase shifter using a pair of multisection radial transmission-line (TL) stubs. The scattering parameters of the differential phase shifter are calculated based on the radial TL theory to evaluate the differential phase shifter's performance. Global optimization is performed using the TL model followed by a local optimization based on a more accurate electromagnetic simulation. The measured operating bandwidth of the circuit is 101.5%, which is larger than many designs in the literature.
Metagenome Mining Reveals Polytheonamides as Posttranslationally Modified Ribosomal Peptides (2012)

It is held as a paradigm that ribosomally synthesized peptides and proteins contain only l-amino acids. We demonstrate a ribosomal origin of the marine sponge-derived polytheonamides, exceptionally potent, giant natural-product toxins. Isolation of the biosynthetic genes from the sponge metagenome revealed a bacterial gene architecture. Only six candidate enzymes were identified for 48 posttranslational modifications, including 18 epimerizations and 17 methylations of nonactivated carbon centers. Three enzymes were functionally validated, which showed that a radical S-adenosylmethionine enzyme is responsible for the unidirectional epimerization of multiple and different amino acids. Collectively, these complex alterations create toxins that function as unimolecular minimalistic ion channels with near-femtomolar activity. This study broadens the biosynthetic scope of ribosomal systems and creates new opportunities for peptide and protein bioengineering.
Reduced transcription of mitochondrial DNA in the senescent rat (1990)

A quantitative study on the effect of senescence on mitochondrial DNA expression has been carried out by measuring the levels of the 12S rRNA and of the mRNA for the subunit I of cytochrome oxidase in several tissues of adult and senescent rats. The concentration of both RNA species/mitochondrial DNA molecule is significantly reduced in senescent brain and heart, as opposed to the respective adult tissues. No appreciable variation occurs in the liver. A 1-h pretreatment with acetyl-L-carnitine brings back the level of senescent brain and heart transcripts to that of adult tissues. The same treatment of adult rats does not cause significant changes in mitochondrial RNA content. These results suggest that the age-dependent impairment of both heavy-strand mitochondrial DNA transcription units is related to altered environmental conditions which acetyl-L-carnitine, a substance which acts by stimulating, directly or indirectly, the energy metabolism, is able to remove.
Bacteremia associated with Enterobacter sakazakii (yellow, pigmented Enterobacter cloacae) (1979)

A case report of bacteremia due to Enterobacter sakazakii, listed previously as yellow-pigmented Enterobacter cloacae (R. Sakazaki, in R. E. Buchanan and N. E. Gibbons, ed., Bergey's Manual of Determinative Bacteriology, 8th ed., p. 325, 1974), occurred in a 7-day-old, Caucasian male who responded successfully to ampicillin therapy. The source of the infection was not known; however, because of the time lapse between birth and the onset of symptoms, the infection was thought to have occurred postnatally.
Joint channel estimation across multiple cells in coordinated multi-point (2012)

Coordinated multi-point transmission (CoMP) allows to deal with interference limitation in cellular systems by acting as a distributed antenna system across cell boundaries. In order to successfully enable CoMP, accurate channel knowledge is required. In our work we focus on uplink joint reception, using linear MMSE combining, suppressing interference. We use LTE-Advanced signal formats together with realistic time-varying frequency-selective channel models. We investigate the performance of different channel estimators in conjunction with several types of Zadoff-Chu-based pilot sequence assignments and different operation points for interference-over thermal noise (IoT) and SIR. The channels are either estimated jointly or separately for all users within the coordinated cell set. We show for Zadoff-Chu sequences that the necessity for joint estimation is much higher for the case of separation by different roots than for the case of separation by different shifts. We also show dependencies of joint estimation gains over IoT and SIR and the interconnection with different approaches for the receive combiner.
Efficient architectures for soft-output algorithms (2002)

Algorithms which compute either the a posteriori probability (APP) or the minimum sequence metric (MSM) of an input symbol to a finite state machine based on some portion of the observation sequence are developed. Full-record (i.e., type-I) and fixed-delay algorithms are both considered and related to existing algorithms. In particular, the fixed-delay APP and MSM algorithms developed are shown to be significantly less complex than the equivalent algorithms of Li, Vucetic, and Sato (1995) i.e. the optimal soft-output algorithm (OSA) and the simplified version (SSA), respectively.
Identification and Characterization of High and Low Affinity Binding Sites for Growth Hormone in Rabbit Liver* (1979)

The specific binding of rat (r) and rabbit (rb) [l25I]iodo-GH was studied in liver membrane preparations isolated from male, virgin female, late pregnant, and midlactating rabbits. Scatchard plots of dose-response displacement curves were consistently nonlinear and suggested the existence of at least two classes of binding sites for both GHs. The average binding affinity constants (Ka) for rGH on the low affinity (1.7 × 108 M-1) and high affinity sites(s) (1.0 × 1010 M-1) were similar to those for the rbGH. Displacement of [125I]iodo-rGH with preparations of GH, PRL, and placental lactogen (PL) from different species demonstrated that the potencies and slopes of the dose-response curves varied considerably, indicating that the displacement activities of many hormones differ at the two sites. Neither the potencies nor the slopes of the dose-response curves correlated with the growth-promoting activities of the various hormones. On the bases of parallelism and potency, rbGH, equine GH, and bovine (b) PRL were the hormones most closely related to the rGH standard. Human (h), ovine (o), and bGH and oPL exhibited steeper slopes of displacement than did rGH and, with the exception of oPL, they had greater potencies. Inhibition of [125I]iodo-rGH binding by oPRL and hPL exhibited lower slope values and lesser potencies than rGH. hPRL, rbPRL, rPRL, and equine PRL effected minimal displacement of [l25]iodo-rGH. The potencies of different hormones relative to the rGH standard varied significantly among different rabbit liver preparations. This variation in apparent potency correlated with the proportion of high affinity sites on the membranes and with the relative affinities of the high and low affinity sites for the different hormones. Neither the proportion of high affinity sites nor the total binding capacity appeared to correlate with the sex or physiological state of the rabbits.
Cloning and expression of 2·5 kb enhancin gene from Trichoplusia ni granulosis virts in Escherichia coli (2001)

The 2. 5 kb fragment of enhancin gene in 3' terminal from Trichoplusia ni granulosis virus was inserted into vector pQE-31 and expressed successfully in Escherichia coli M15 (p RE P_(4)).The synergy of the expression product(P96) on Helicoverpa armiyera nuclear polyhedrosis virus(HaNPV)against the 3 rd instar larvae of H.armigera was also studied.The results indicated that the percentage of accumulated mortality of the larvae increased by 27. 40%-34. 50%on the 11 th day postinfection and the median lethal time decreased at least by 1.9 days in HaNP V+P96 treated compared with those in HaNPV treated.
MODEL FORMULATION FOR FLEET SIZE ANALYSIS OF A UNIVERSITY MOTOR POOL (1979)

ABSTRACT A GPSS/360 model was developed to investigate the behavior of a university's motor pool dispatch fleet. Time‐series data were collected and frequency distributions were constructed for vehicle request patterns and trip‐duration times. Regression analysis was performed to formulate trip mileage generators as functions of service time. Appropriate statistical goodness‐of‐fit tests were conducted to ascertain the extent of congruence between actual system behavior and expectations based on a number of theoretical distributions. Analysis of simulated response variables indicates that the model's internal structure reproduces reality to a high degree. Fifteen years of simulated experience were generated for six alternative fleet size configurations; results suggest that reductions in existing fleet capacity could be realized without effecting undue impairment in service levels.
Alternative splicing variant of the hypoxia marker carbonic anhydrase IX expressed independently of hypoxia and tumour phenotype (2007)

CA IX is a hypoxia-induced, cancer-associated carbonic anhydrase isoform with functional involvement in pH control and cell adhesion. Here we describe an alternative splicing variant of the CA9 mRNA, which does not contain exons 8-9 and is expressed in tumour cells independently of hypoxia. It is also detectable in normal tissues in the absence of the full-length transcript and can therefore produce false-positive data in prognostic studies based on the detection of the hypoxia- and cancer-related CA9 expression. The splicing variant encodes a truncated CA IX protein lacking the C-terminal part of the catalytic domain. It shows diminished catalytic activity and is intracellular or secreted. When overexpressed, it reduces the capacity of the full-length CA IX protein to acidify extracellular pH of hypoxic cells and to bind carbonic anhydrase inhibitor. HeLa cells transfected with the splicing variant cDNA generate spheroids that do not form compact cores, suggesting that they fail to adapt to hypoxic stress. Our data indicate that the splicing variant can functionally interfere with the full-length CA IX. This might be relevant particularly under conditions of mild hypoxia, when the cells do not suffer from severe acidosis and do not need excessive pH control.
Deposition Strategies and Resulting Part Stiffnesses in Fused Deposition Modeling (1999)

In the Fused Deposition Modeling (FDM) process, the choice of deposition strategy plays an important role. In this paper, the effects of different deposition paths on this deposition based LM process are investigated. Some variations on the current deposition strategies are also proposed. The stiffness of parts manufactured by the different strategies is experimentally determined. It is then compared with an analytical model developed using laminate analysis. A good conformance of the laminate model to the experimental results suggests that the laminate model can be used as a design aide to help the designer tailor the deposition strategy to the stiffness requirements.
“GOD TALK” IS IT HEALTHYOR UNHEALTHY? (1986)

Most Christians, at least initially, tend to think that all talk about God is healthy and an open door to share their own faith. Many non-Christian therapists believe that all talk
Mujer y vivienda: una aproximación al problema de la vivienda desde una perspectiva de género (2003)

Este trabajo de investigacion tiene como objetivo la elaboracion de un conjunto de propuestas y lineas de actuacion para introducir la perspectiva de genero en la politica de vivienda espanola. El trabajo se centra en tres campos estrechamente relacionados: las condiciones socioeconomicas y residenciales de las mujeres espanolas, el contexto normativo vigente en Espana en materia de politica de vivienda y de genero, y una revision de las experiencias internacionales mas interesantes que se han llevado a cabo en este campo.
Control and function of lysyl‐tRNA synthetases: diversity and co‐ordination (1993)

Lysyl-tRNA synthetases are synthesized from two distinct genes in Escherichia coli, lysS (constitutively) and lysU (inducibly); however, the physiological significance and the differential control mechanism of these two genes have been a long-standing puzzle. Recent studies have successfully uncovered a significant control mechanism of lysU expression, which involves the leucine-responsive regulatory protein (Lrp) and a translational enhancer element called 'downstream box'. Moreover, it is likely that there is a mechanism underlying co-ordinate expression of lysU with other genes outside the leucine-Lrp regulon under harsh conditions such as low pH and anaerobiosis. A possible mechanism of lysyl-tRNA synthetase expression and function is reviewed.
Intrinsically Microporous Poly(imide)s: Structure−Porosity Relationship Studied by Gas Sorption and X-ray Scattering (2011)

Intrinsically microporous soluble poly(imide)s were prepared via polycondensation from (±)-2,2′-diamino-1,1′-binaphthalene or 4,4′-(9-fluorenylidene)dianiline and four different linear comonomers: pyromellitic dianhydride, 3,3′,4,4′-biphenyltetracarboxylic dianhydride, 4,4′-oxidiphthalic anhydride, and 4,4′-(hexafluoroisopropylidene)diphthalic anhydride. The porosity of the resulting eight poly(imide)s was determined by nitrogen, argon, hydrogen, and carbon dioxide sorption for precipitated and cast samples and compared. While only the stiffest and most porous poly(imide)s were able to adsorb nitrogen and argon, almost all polymers were able to adsorb hydrogen and carbon dioxide. On the basis of the sorption results, it was possible to determine the limit of intrinsic microporosity. The processing of the polymers has a major influence on the porosity as investigated by gas sorption and X-ray scattering. Solvent cast films are denser and can therefore be regarded as thermodynamically stable. Interestingly, we observed selective gas sorption phenomena (high carbon dioxide sorption but low hydrogen sorption) for some of the polymers. Finally, we investigated the response of one poly(imide) thin film to changing gas atmosphere by ellipsometry.
BH InGaAsP Lasers with an LPE Grown Semi-Insulating Layer (1986)

DCPBH lasers with LPE grown cobalt-doped semi-insulating InP layer for lateral current confinement are described. The lasers with this cobalt-InP layer have higher external quantum efficiencies than the ones without. Low threshold current lasers (15–35 mA) are obtained for active region width of 2–3 µm.
E2F and p53 Induce Apoptosis Independently during Drosophila Development but Intersect in the Context of DNA Damage (2008)

In mammalian cells, RB/E2F and p53 are intimately connected, and crosstalk between these pathways is critical for the induction of cell cycle arrest or cell death in response to cellular stresses. Here we have investigated the genetic interactions between RBF/E2F and p53 pathways during Drosophila development. Unexpectedly, we find that the pro-apoptotic activities of E2F and p53 are independent of one another when examined in the context of Drosophila development: apoptosis induced by the deregulation of dE2F1, or by the overexpression of dE2F1, is unaffected by the elimination of dp53; conversely, dp53-induced phenotypes are unaffected by the elimination of dE2F activity. However, dE2F and dp53 converge in the context of a DNA damage response. Both dE2F1/dDP and dp53 are required for DNA damage-induced cell death, and the analysis of rbf1 mutant eye discs indicates that dE2F1/dDP and dp53 cooperatively promote cell death in irradiated discs. In this context, the further deregulation in the expression of pro-apoptotic genes generates an additional sensitivity to apoptosis that requires both dE2F/dDP and dp53 activity. This sensitivity differs from DNA damage-induced apoptosis in wild-type discs (and from dE2F/dDP-induced apoptosis in un-irradiated rbf1 mutant eye discs) by being dependent on both hid and reaper. These results show that pro-apoptotic activities of dE2F1 and dp53 are surprisingly separable: dp53 is required for dE2F-dependent apoptosis in the response to DNA damage, but it is not required for dE2F-dependent apoptosis caused simply by the inactivation of rbf1.