gebhart/patrepeval huggingface dataset:
DEVELOPMENTAL GENETICS OF THE 2C-D REGION OF THE DROSOPHILA <i>X</i> CHROMOSOME (1985)

We have conducted a genetic and developmental analysis of genes within the 2C-D area of the X chromosome. Phenotypes of 33 mutations representing nine adjacent complementation groups including eight recessive lethals and one visible homeotic mutation (polyhomeotic) are described. Germline clonal analysis of the eight zygotic lethals has revealed three types of gene requirements: normal activity at two pupal lethal loci (corkscrew and C204) and one larval lethal locus (ultraspiracle) is required for normal embryogenesis; normal activity at three larval lethal loci (DF967, VE651 and Pgd) is required for normal oogenesis; and activity at only one locus (EA82), a larval lethal, appears to have no maternal requirement. Ambiguous results were obtained for the GF316 lethal complementation group. Analysis of mitotic figures of the pupal lethals indicates that C204 disrupts an essential mitotic function. This result correlates with the preblastoderm arrest observed among embryos derived from germline clones of C204. Embryos derived from germline clones of corkscrew (csw) exhibit a "twisted" phenotype. The recessive lethal ultraspiracle (usp) disrupts the organization of the posterior tip of the larval both zygotically and maternally: second instar usp/Y larvae derived from heterozygous usp/+ mothers possess an extra set of spiracles, whereas usp/Y embryos derived from females possessing a germline clone (usp/usp) exhibit a localized ventral defect in the ninth or posterior eighth abdominal segment. Analysis of the phenotypes of deficiency-hemizygous embryos indicates the presence of an embryonic zygotic lethal locus, as yet unidentified, which produces central nervous system and ventral hypoderm degeneration. Additional information on the genetic organization of loci within the adjacent 2E area are also described.(ABSTRACT TRUNCATED AT 250 WORDS)
Functional Domains of the SYT and SYT-SSX Synovial Sarcoma Translocation Proteins and Co-Localization with the SNF Protein BRM in the Nucleus (1999)

The t(X;18)(p11.2;q11.2) chromosomal translocation commonly found in synovial sarcomas fuses the SYT gene on chromosome 18 to either of two similar genes, SSX1 or SSX2, on the X chromosome. The SYT protein appears to act as a transcriptional co-activator and the SSX proteins as co-repressors. Here we have investigated the functional domains of the proteins. The SYT protein has a novel conserved 54 amino acid domain at the N-terminus of the protein (the SNH domain) which is found in proteins from a wide variety of species, and a C-terminal domain, rich in glutamine, proline, glycine and tyrosine (the QPGY domain), which contains the transcriptional activator sequences. Deletion of the SNH domain results in a more active transcriptional activator, suggesting that this domain acts as an inhibitor of the activation domain. The C-terminal SSX domain present in SYT-SSX translocation protein contributes a transcriptional repressor domain to the protein. Thus, the fusion protein has transcriptional activating and repressing domains. We demonstrate that the human homologue of the SNF2/Brahama protein BRM co-localizes with SYT and SYT-SSX in nuclear speckles, and also interacts with SYT and SYT-SSX proteins in vitro. This interaction may provide an explanation of how the SYT protein activates gene transcription.
Identification and characterization of 4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-<i>5H</i>-pyrimido[5,4-b]indole derivatives as<i>Salmonella</i>biofilm inhibitors (2012)

A screening of a small-molecule library was conducted, in search of Salmonella biofilm inhibitors active in a broad temperature range, both in prevention and in eradication of biofilms. Moreover, the inhibitors were selected not to influence the planktonic growth of Salmonella to diminish the selective pressure and to prevent or slow down resistance development. Out of the 20,014 compounds screened at 16 and 37 °C, 140 hits were identified. After characterization of the most promising hits at a broader set of temperatures (16, 25, 30 and 37 °C), we identified 7-methoxy-4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-5H-pyrimido[5,4-b]indole as an interesting preventive anti-biofilm compound. A first structure-activity relationship of this compound was delineated, revealing 8-fluoro-4-[4-(3-phenyl-2-propen-1-yl)-1-piperazinyl]-5H-pyrimido[5,4-b]indole as a promising analogue in the prevention of Salmonella biofilms.
Increased Functional Half-life of Fibroblast Growth Factor-1 by Recovering a Vestigial Disulfide Bond (2013)

Abstract: The fibroblast growth factor (FGF) family of proteins contains an absolutely conserved Cys residue at position 83 that is present as a buried free cysteine. We have previously shown that mutation of the structurally adjacent residue, Ala66, to cysteine results in the formation of a stabilizing disulfide bond in FGF-1. This result suggests that the conserved free cysteine residue at position 83 in the FGF family of proteins represents a vestigial half-cystine. Here, we characterize the functional half-life and mitogenic activity of the oxidized form of the Ala66Cys mutation to identify the effect of the recovered vestigial disulfide bond between Cys83 and Cys66 upon the cellular function of FGF-1. The results show that the mitogenic activity of this mutant is significantly increased and that its functional half-life is greatly extended. These favorable effects are conferred by the formation of a disulfide bond that simultaneously increases thermodynamic stability of the protein and removes a reactive buried thiol at position 83. Recovering this vestigial disulfide by introducing a cysteine at position 66 is a potentially useful protein engineering strategy to improve the functional half-life of other FGF family members.
Ultra-low-power stress-optics modulator for microwave photonics (2017)

In this work, we demonstrate the first stress-optic modulator in a silicon nitride-based waveguide platform (TriPleX) in the telecommunication C-band. In our stress-optic phase modulator the refractive index of the waveguiding materials is controlled by the stress-optic effect induced by actuating a 2 μm thick PZT layer on top of the TriPleX waveguide geometry. The efficiency of the modulator is optimized by, amongst others, focusing the applied stress in the waveguide core region through a local increase of the top cladding. Using a Mach-Zehnder interferometer, we measured a half-wave voltage, V<sub>π</sub>, at 34 V at a wavelength of 1550 nm using a modulator with a total length of 14.8 mm. The measured static power consumption of our stress-optic modulator is in the μW-region as it is only determined by small leakage currents (< 0.1 μA), while the dynamic power consumption at a rise time of 1 ms (1 kHz excitation) is less than 4 mW per modulator. The stress optical modulator goes with an excess loss of 0.01 dB per modulator only. This is in line with the typical low loss characteristics of TriPleX waveguides, being < 0.1 dB/cm at a wavelength of 1550 nm. These specifications make stress-optic modulators an excellent choice for next generation optical beam forming networks with a large number of actuators in silicon photonics in general and in the TriPleX platform in particular.
Embossing of optical document security devices (2004)

Embossing in the transparent window area of polymer banknotes, such as those seen on the Australian, New Zealand and Romanian currencies, have enormous potential for the development of novel optical security devices. The intaglio printing process can provide an efficient means for embossing of optical security structures such as micro lenses. Embossed micro lens arrays in the transparent window of a polymer banknote can be folded over a corresponding printed image array elsewhere on the note to reveal a series of moire magnified images. Analysis of samples of embossed micro lenses showed that the engraving side and impression side had a similar embossed profile. The embossed micro lens profiles were modelled using Optalix-LX commercial optical ray tracing software in order to determine the focal length of the lenses and compare with the focal length of desired embossed lenses. A fundamental understanding of how the polymer deforms during the embossing process is critical towards developing a micro lens embossing tool which can achieve the desired embossed micro lenses. This work also looks at extending the early research of the Intaglio Research Group (IRG) to better understand the embossibility of polymer substrates such as biaxially oriented polypropylene (BOPP).
Chromatin structure is required to block transcription of the methylated herpes simplex virus thymidine kinase gene. (1987)

Inhibition of herpes simplex virus (HSV) thymidine kinase (TK) gene transcription (pHSV-106, pML-BPV-TK4) by DNA methylation is an indirect effect, which occurs with a latency period of approximately equal to 8 hr after microinjection of the DNA into TK- rat 2 and mouse LTK- cells. We have strong evidence that chromatin formation is critical for the transition of the injected DNA from methylation insensitivity to methylation sensitivity. Chromatin was reconstituted in vitro by using methylated and mock-methylated HSV TK DNA and purified chicken histone octamers. After microinjection, the methylated chromatin was always biologically inactive, as tested by autoradiography of the cells after incubation with [3H]thymidine and by RNA dot blot analysis. However, in transformed cell lines, reactivation of the methylated chromatin occurred after treatment with 5-azacytidine. Furthermore, integration of the TK chromatin into the host genome is not required to block expression of the methylated TK gene. Mouse cells that contained the pML-BPV-TK4 chromatin permanently in an episomal state also did not support TK gene expression as long as the TK DNA remained methylated.
Plasma phospho‐tau181 increases with Alzheimer's disease clinical severity and is associated with tau‐ and amyloid‐positron emission tomography (2018)

Plasma pTau181 may have utility as a biomarker of AD pathophysiology and as a noninvasive screener for elevated brain Aβ.
Matrix Metalloproteinase-9 Facilitates Remyelination in Part by Processing the Inhibitory NG2 Proteoglycan (2003)

Remyelination is a critical repair process that is initiated after a demyelinating insult. The failure to remyelinate contributes to neurological diseases such as multiple sclerosis. Here, we test the hypothesis that proteinase activity is required for the extensive remodeling of the extracellular matrix that occurs during remyelination. We show that mice lacking matrix metalloproteinase (MMP)-9 are impaired in myelin reformation after lysolecithin-induced demyelination. This deficiency may be explained at least in part by the failure to clear the accumulation of NG2, an inhibitory proteoglycan that retards the maturation and differentiation of oligodendrocytes that are needed for remyelination. These results emphasize for the first time that upregulation of MMP activity can be important for facilitating regeneration from some types of CNS injury.
Optical-parametric-amplification imaging of complex objects (2011)

We used ultrafast Fourier-plane optical-parametric-amplification (OPA) imaging to simultaneously image, wavelength-shift, and amplify complex two-dimensional objects with spatial features from 1.1 to 10.1 line pairs/millimeter (lp/mm) in the vertical dimension and from 2.0 to 16.0 lp/mm in the horizontal dimension, corresponding to a two-dimensional space-bandwidth product (SBP) of ~46,000. This represents an increase in image complexity over previous analogous OPA imaging systems by over three orders of magnitude. We observe both wavelength-shifting the image from 930 nm to a wavelength of 700 nm and image amplification by two orders of magnitude. Our wavelength-shifted image has a SBP of ~30,000.
Experimental and Numerical Investigation of Structural Damage Detection Using Changes in Natural Frequencies (2007)

A robust iterative algorithm is used to identify the locations and extent of damage in beams using only the changes in their first several natural frequencies. The algorithm, which combines a first-order, multiple-parameter perturbation method and the generalized inverse method, is tested extensively through experimental and numerical means on cantilever beams with different damage scenarios. If the damage is located at a position within 0–35% or 50–95% of the length of the beam from the cantilevered end, while the resulting system equations are severely underdetermined, the minimum norm solution from the generalized inverse method can lead to a solution that closely represents the desired solution at the end of iterations when the stiffness parameters of the undamaged structure are used as the initial stiffness parameters. If the damage is located at a position within 35–50% of the length of the beam from the cantilevered end, the resulting solution by using the stiffness parameters of the undamaged structure as the initial stiffness parameters deviates significantly from the desired solution. In this case, a new method is developed to enrich the measurement information by modifying the structure in a controlled manner and using the first several measured natural frequencies of the modified structure. A new method using singular value decomposition is also developed to handle the ill-conditioned system equations that occur in the experimental investigation by using the measured natural frequencies of the modified structure.
Next Generation Light Sources for Biomedical Applications (2008)

Abstract Lasers are increasingly important components within biological imaging and analysis systems. Applications range from flow cytometry to confocal and multi‐photon microscopy. However, many commercial imaging systems are still limited in performance by the availability of suitable laser sources‐limited in the availability of wavelengths and in the size, cost, power and reliability of conventional laser technologies. Ultrafast fibre lasers offer turnkey, compact and reliable solutions for next generation biomedical imaging systems.
Renal cell carcinoma metastatic to the thyroid (1990)

Metastatic renal cell carcinoma to the thyroid is an uncommon occurrence that can cause clinical and pathologic problems in diagnosis. The authors report seven cases from the files of The University of Texas M.D. Anderson Cancer Center, Houston, Texas. Each presented clinically as a palpable thyroid nodule months or years after the primary renal cell carcinoma had been resected. Although renal cell carcinoma is more common in men, we found a female predominance of 6:1 in this series. These lesions appear as solitary "cold" nodules on iodine 131 scans and may be misdiagnosed as primary thyroid neoplasms, especially if the renal primary is still unrecognized. A correct diagnosis is important because surgical management can be curative. The diagnosis is facilitated by the use of fat stains, electron microscopic study, and immunoperoxidase stains.
Multi-Partition RAID: A New Method for Improving Performance of Disk Arrays under Failure (1997)

Disk arrays have been proposed as a way of improving I/O performance by using parallelism among multiple disks. This paper focuses, however, on improving the performance of disk array systems in the presence of disk failures, which are significant for applications where continuous operation is of concern. Although several approaches have been explored, the goals of achieving high performance and storage efficiency often conflict. In this paper, we propose a new variation of RAID organization, multi-partition RAID (mP-RAID), to improve storage efficiency and reduce performance degradation when disk failures occur. The idea is to recognize that frequently demanded data dominate the degree of the performance degradation when disk failures occur. mP-RAID subdivides a disk array into several partitions associated with different block organizations. Based upon data popularity, we assign data to appropriate partitions so that high performance and better storage efficiency can be achieved simultaneously.
Evaluation of proton conductivity of sulfonated polyimide/dihydroxy naphthalene charge-transfer complex hybrid membranes (2014)

Sulfonated polyimide (SPI)/dihydroxynaphthalene (DHN) charge-transfer (CT) complex hybrid films were investigated as possible alternative for polymer electrolyte membranes in polymer electrolyte fuel cells. SPI/DHN CT complex hybrid films include CT complexes, which might work as electronic conductors, and sulfonic acid units, which could work as proton conductors. Therefore, the origin of the conductivity of SPI/DHN complex hybrid films was evaluated by four-probe impedance measurements in the through-plane direction of the films. The obtained conductivity of the CT complex hybrid films increased with the increase of ion exchange capacity of the CT films and the decrease of CT complex concentration in the films. These results indicated that proton transfer dominantly occurred in the CT complex hybrid films. Proton conductivity of the CT complex hybrid films consisting of 2,6- or 1,5-DHN showed the similar values, although the molecular geometries of the CT complex were different. The activation energy values for proton conductivity in the CT films were approximately the same as that of Nafion 212. Water uptake (WU) results were also conducted and suggest that CT complex formation could control the degree of WU of the films and prevent dissolution of SPI. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 2991–2997
China in 1985: The Greening of the Revolution (1986)

Research Article| January 01 1986 China in 1985: The Greening of the Revolution Richard Baum Richard Baum Search for other works by this author on: This Site PubMed Google Scholar Far Eastern Survey (1957) 26 (1): 30–53. https://doi.org/10.2307/2644092 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter LinkedIn Tools Icon Tools Get Permissions Cite Icon Cite Search Site Citation Richard Baum; China in 1985: The Greening of the Revolution. Far Eastern Survey 1 January 1957; 26 (1): 30–53. doi: https://doi.org/10.2307/2644092 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAsian Survey Search This content is only available via PDF. Copyright 1986 The Regents of the University of California Article PDF first page preview Close Modal You do not currently have access to this content.
Prevention of Escherichia coli infection in broiler chickens with a bacteriophage aerosol spray (2002)

Bacteriophage to an Escherichia coli isolate that is pathogenic in poultry were isolated from municipal sewer treatment facilities or poultry processing plants. Three studies were conducted to determine the efficacy of aerosol administration of bacteriophage to prevent an E. coli respiratory infection in broiler chickens. In all three studies the experimental design consisted of nine treatments with three replicate pens of 10 birds. Three treatments were not challenged with E. coli and consisted of unsprayed birds, birds sprayed with a diluent control, and birds sprayed with a combination of two bacteriophages. Six treatments were challenged with E. coli by injecting 10(4) cfu into the thoracic air sac when birds were 7, 8, or 10 d of age after being sprayed at 7 d of age with either a diluent control or a combination of two bacteriophages. In Studies 1 and 2, BW at 2 wk of age of all the birds challenged with E. coli, regardless of spray treatment, were decreased significantly from the unchallenged controls, except in Study 2 for the birds sprayed with bacteriophage and challenged at 10 d of age. There was a significant decrease in mortality in Studies 1 and 2 when the birds were challenged with E. coli immediately after bacteriophage administration and in Study 2 in birds challenged at 10 d of age. In Study 3 a suspected pre-existing E. coli infection resulted in mortality in the unchallenged, unsprayed controls, and in the diluent sprayed controls of 20 and 27%, respectively. The mortality in the unchallenged bacteriophage sprayed birds was 3%, representing a significant decrease. Mortality in Study 3 was significantly decreased in the bacteriophage-sprayed birds challenged with E. coli immediately or 1 d later but not 3 d after bacteriophage administration. The decrease in BW at 2 wk of age in challenged birds indicates that bacteriophage treatment did not provide complete protection; however, in all three studies mortality was significantly decreased, indicating that aerosol spray of bacteriophage may be practical for administration of bacteriophage and may provide an alternative to the use of antibiotics in poultry production.
Comprex® Pressure-Wave Supercharging for Automotive Diesels-State-of-the-Art (1984)

<div class="htmlview paragraph">THE COMPREX PRESSURE WAVE SUPERCHARGER (PWS), developed for passenger car diesel engines, is now in production. Those of its properties which influence road performance, fuel consumption, and emission behavior of the PWS charged engine are described on the basis of the maps of the production models. The design for high volume production is presented and substantiated by test results.</div> <div class="htmlview paragraph">6 types are available, covering an engine range from one to three litr swept volume with and without charge air cooling and optional waste gate. Instant response and high efficiency allow downsizing and downspeeding and lead to improved fuel economy, low emission and low noise level.</div>
Binding Site for Blood Coagulation Factor Xa Involving Residues 311–325 in Factor Va (1998)

Factor Va inactivation by activated protein C is associated with cleavages at Arg306, Arg506, and Arg679 with Arg306 cleavage causing the major activity loss. To study functional roles of the Arg306 region, overlapping 15-mer peptides representing the sequence of factor Va residues 271-345 were synthesized and screened for anticoagulant activities. The peptide containing residues 311-325 (VP311) noncompetitively inhibited prothrombin activation by factor Xa, but only in the presence of factor Va. Fluorescence studies showed that VP311 bound to fluorescence-labeled 5-dimethylaminonaphthalene-1-sulfonyl-Glu-Gly-Arg factor Xa in solution with a Kd of 70 microM. Diisopropylphosphoryl factor Xa and factor Xa but not factor VII/VIIa or prothrombin bound to immobilized VP311 with relatively high affinity. These results support the hypothesis that residues 311-325, which are positioned between the A1 and A2 domains of factor Va and likely exposed to solvent, contribute to the binding of factor Xa by factor Va. Based on this hypothesis, it is suggested that cleavage by activated protein C at Arg306 in factor Va not only severs the covalent connection between the A1 and A2 domains but also disrupts the environment and structure of residues 311-325, thereby down-regulating the binding of factor Xa to factor Va.
Regularized image up-sampling using a new observation model and the level set method (2004)

This paper presents a new formulation of the regularized image up-sampling problem that incorporates models of the image acquisition and display processes. This approach leads to a new data fidelity term that has been coupled with a bounded-total-variation regularizer to yield our objective function. This objective function is minimized using the level-set method with two types of motion that interact simultaneously. The method was implemented and has been verified to provide good results, yielding crisp edges without introducing ringing or other artifacts.