gebhart/patrepeval huggingface dataset:
Split-flow Control and Optimization of Campus Network Traffic (2014)

Taking the campus network of Longdong University in Gansu province as research object, and aiming at the existing problems such as unreasonable bandwidth usage and link congestion, this article proposed to deploy professional flow control equipment to implement split-flow, optimize the application strategy and improve the network security protection. It has been proved that, using this scheme could significantly improve the campus network bandwidth, which strongly ensured the key network applications including teaching, research and management.
The adenosinergic system in cancer (2013)

High amounts of adenosine are released in the tumor mass. Depending on the levels of adenosine, as well as on the receptor subtypes that are expressed by immune cells, adenosine can affect tumor growth in different fashions. Specifically targeting CD73, the rate-limiting enzyme for the extracellular generation of adenosine, or the A3 receptor offers new therapeutic strategies to limit tumor progression.
Screw Thread Machining of Composite Materials Using Abrasive Waterjet Cutting (1994)

Abstract An investigation was performed to study the mechanisms of abrasive wateijet cutting of unidirectional carbon/epoxy composite rods. The objective of the study was to determine the feasibility of using abrasive waterjet cutting to machine high quality, standard dimension screw threads. Experimental trials were performed to identify suitable cutting conditions for machining threads in unidirectional, carbon/epoxy rods. Parameters including traverse speed, crossfeed and nozzle / orifice dimensions were varied and conditions providing high quality screw threads were determined. Scanning electron microscope (SEM) examinations of the machined threads revealed different surface characteristics at different locations along the thread profile. Evidence of several composite failure mechanisms, including fiber-matrix interface failure, fiber pullout and fiber failure, was observed. It is concluded that through careful control of the abrasive waterjet machining parameters, high quality screw threads can be obtained.
Ubiquitination of α-Synuclein (2004)

Filamentous alpha-synuclein depositions are the defining hallmarks of a subset of neurodegenerative diseases including Parkinson's disease (PD), dementia with Lewy bodies, and multiple system atrophy. We previously reported that alpha-synuclein in those brains are extensively phosphorylated at Ser129 [Fujiwara et al. (2002) Nat. Cell Biol. 4, 160-164] and also partially ubiquitinated [Hasegawa et al. (2002) J. Biol. Chem. 277, 49071-49076]. Here, we investigate ubiquitination of alpha-synuclein in vitro and in vivo and report the ubiquitination sites and the effects of familial PD-linked mutations, phosphorylation, and fibril formation on ubiquitination. Protein-sequence analysis revealed that Lys21, Lys23, Lys32, and Lys34 within the repeats in the amino-terminal half are liable to ubiquitination in vitro. A site-directed mutagensis study confirmed that these are the major ubiquitination sites. A53T and A30P mutations had no significant effect on ubiquitination. Similarly, phosphorylation of alpha-synuclein at Ser129 did not affect ubiquitination. Notably, we show that assembled, filamentous alpha-synuclein is less ubiquitinated than the soluble form and that the major ubiquitination sites are localized to Lys6, Lys10, and Lys12 at the amino-terminal region of filamentous alpha-synuclein. Furthermore, we successfully detected ubiquitination of alpha-synuclein in 293T cells by cotransfection with alpha-synuclein and ubiquitin. The in vivo ubiquitination sites were found to be identical to those in filamentous alpha-synuclein. PD-linked mutations and phosphorylation at Ser129 had no effects on ubiquitination of alpha-synuclein in vivo. These data may have implications for the mechanisms of the formation of alpha-synuclein deposits in alpha-synucleinopathy brains.
Heparan sulfate proteoglycans mediate Aβ-induced oxidative stress and hypercontractility in cultured vascular smooth muscle cells (2016)

Taken together, our data indicate that HSPG are critical mediators of Aβ-induced oxidative stress and Aβ(1-40⁻)induced VSMC dysfunction.
Characterization of distinct types of KRAS mutation and its impact on first‑line platinum‑based chemotherapy in Chinese patients with advanced non‑small cell lung cancer (2017)

We performed this retrospective study to investigate whether the KRAS mutation status and its subtypes could predict the effect of first-line platinum-based chemotherapy in Chinese patients with non-small cell lung cancer (NSCLC). Patients received who had KRAS mutations were enrolled. Correlations between KRAS mutations, specific mutant subtypes and responses to chemotherapy were analyzed using Kaplan-Meier and Cox proportional hazard methods. A total of 2,183 cases who received KRAS mutation detection were included. A total of 218 of these cases were indicated to have KRAS mutations. KRAS mutations were identified more commonly in males compared with females (P=0.035). The most common subtypes were G12C, G12D and G12V. Among 73 KRAS mutant patients and 100 EGFR/ALK/KRAS wild-type patients with advanced NSCLC, KRAS-mutant NSCLC patients had a significantly shorter progression-free survival (P=0.007) compared with NSCLC patients with KRAS wild-type. In addition, there was a shorter but marginally statistically significant progression-free survival (PFS) in KRAS mutant patients with adenocarcinoma compared with those with non-adenocarcinoma (P=0.051). In the KRAS mutant group, patients with the KRAS G12V mutation had the poorest PFS compared with non-G12V mutant cases (P=0.045). In conclusion, KRAS mutation was a negative predictive factor of PFS in Chinese patients with advanced NSCLC who received first platinum-based chemotherapy. Patients with KRAS G12V mutations exhibited the poorest PFS compared with those with other KRAS mutant types.
Selective modulation through the glucocorticoid receptor ameliorates muscle pathology in <i>mdx</i> mice (2013)

The over-expression of NF-κB signalling in both muscle and immune cells contribute to the pathology in dystrophic muscle. The anti-inflammatory properties of glucocorticoids, mediated predominantly through monomeric glucocorticoid receptor inhibition of transcription factors such as NF-κB (transrepression), are postulated to be an important mechanism for their beneficial effects in Duchenne muscular dystrophy. Chronic glucocorticoid therapy is associated with adverse effects on metabolism, growth, bone mineral density and the maintenance of muscle mass. These detrimental effects result from direct glucocorticoid receptor homodimer interactions with glucocorticoid response elements of the relevant genes. Compound A, a non-steroidal selective glucocorticoid receptor modulator, is capable of transrepression without transactivation. We confirm the in vitro NF-κB inhibitory activity of compound A in H-2K(b) -tsA58 mdx myoblasts and myotubes, and demonstrate improvements in disease phenotype of dystrophin deficient mdx mice. Compound A treatment in mdx mice from 18 days of post-natal age to 8 weeks of age increased the absolute and normalized forelimb and hindlimb grip strength, attenuated cathepsin-B enzyme activity (a surrogate marker for inflammation) in forelimb and hindlimb muscles, decreased serum creatine kinase levels and reduced IL-6, CCL2, IFNγ, TNF and IL-12p70 cytokine levels in gastrocnemius (GA) muscles. Compared with compound A, treatment with prednisolone, a classical glucocorticoid, in both wild-type and mdx mice was associated with reduced body weight, reduced GA, tibialis anterior and extensor digitorum longus muscle mass and shorter tibial lengths. Prednisolone increased osteopontin (Spp1) gene expression and osteopontin protein levels in the GA muscles of mdx mice and had less favourable effects on the expression of Foxo1, Foxo3, Fbxo32, Trim63, Mstn and Igf1 in GA muscles, as well as hepatic Igf1 in wild-type mice. In conclusion, selective glucocorticoid receptor modulation by compound A represents a potential therapeutic strategy to improve dystrophic pathology.
The maize RNA‐binding protein, MA16, is a nucleolar protein located in the dense fibrillar component (1994)

Summary A developmentally and environmentally regulated gene in maize, MA 16, encoding an RNA‐binding protein that binds preferentially to uridine and guanosine‐rich RNAs has previously been described. To gain some insight into the function of MA 16 the distribution of MA 16 mRNA and protein during maize development was investigated using in situ hybridization, RNA and protein gel blot analysis and immunocytochemistry. The results show that MA16 is expressed throughout development of the embryo and seedling in different tissues and at different levels. The level of MA 16 mRNA is higher in developing and expanding structures such as the root elongation zone and young leaves. After stress treatment MA 16 mRNA increases in total and polysomal RNA, but no significant change in the level of the protein was detected. MA16 is a non‐ribosomal nucleolar protein. Using immunoelectron microscopy the MA16 protein has been located in the dense fibrillar component and to a lesser extent in the granular component of the nucleolus. It was found that MA16 contains the conserved sequence motifs R(G) n Y(G) n R and RR(E/D)(G) n Y(G) n repeated in the C‐terminal of the molecule that conforms imperfectly to the GAR motif proposed for nucleolar proteins. In light of these results the stress regulation of MA 16 and a likely role for this protein in pre‐rRNA processing and/or ribosome assembly is discussed.
Changes and Structure-New Drives,New BaLance,New Orientation (2012)

Global textile and apparel industry has been experiencing a worldwide structural adjustment.The textile and apparel industry in China not only would confront with the comparatively-lower cost in the south-east Asian countries, but also would cope with
Order forecasts, retail sales, and the marketing mix for consumer durables (1998)

The paper examines the problem of forecasting ongoing factory orders and monitoring retail demand, with specific reference to high-technology consumer durables. We present evidence of the managerial importance of the problem and, using a case study of a computer peripheral manufacturer, we describe how different data sources and models can be used to increase prediction accuracy. First we examine the order placement and retail demand process using extrapolative methods that focus on identifying short- versus long-run movements in orders. We then introduce marketing-mix data for improved retail demand tracking and forecasting, and we propose the use of conjoint measurement data to simulate a product's utility over time and include that information in the demand model. Lastly, we describe the forecasting and marketing planning use of these models and discuss their implications. © 1998 John Wiley & Sons, Ltd.
Preparation of transparent conducting B-doped ZnO films by vacuum arc plasma evaporation (2007)

Highly transparent and conductive B-doped ZnO (BZO) thin films have been prepared by a newly developed vacuum arc plasma evaporation method that provided high-rate film depositions using sintered BZO pellets and fragments. The obtained electrical and optical properties of the deposited BZO thin films were considerably affected by the deposition conditions as well as the preparation method of the BZO pellets and fragments used. The lowest thin film resistivity was obtained with a B doping content [B∕(B+Zn) atomic ratio] of approximately 1at.%. A resistivity as low as 5×10−4Ωcm and an average transmittance above about 80% in the wavelength range of 400–1300nm were obtained in BZO films prepared with a thickness above approximately 400nm at a substrate temperature of 200°C. In addition, a low resistivity of 7.97×10−4Ωcm and average transmittances above about 80% in the visible wavelength range were obtained in a BZO film prepared at a substrate temperature of 100°C and an O2 gas flow rate of 10SCCM (SCCM denotes cubic centimeter per minute at STP). The deposition rate of BZO films was typically 170nm∕min with a cathode plasma power of 4.5kW.
The CRISPR endoribonuclease Csy4 utilizes unusual sequence- and structure-specific mechanisms to recognize and process crRNAs (2012)

Many prokaryotes contain clustered regularly interspaced short palindromic repeats (CRISPRs) that together with CRISPR-associated (<italic>cas</italic>) genes confer resistance to invasive genetic elements. Central to this immune system is the production of CRISPR-derived RNAs (crRNAs) via enzymatic cleavage of CRISPR locus transcripts. These crRNAs serve as guides for foreign nucleic acid targeting and degradation. Here we identify Csy4 as the endoribonuclease responsible for CRISPR transcript processing in <italic>Pseudomonas aeruginosa</italic> UCBPP-PA14. Biochemical assays and six co-crystal structures of Csy4 bound to substrate and product crRNAs reveal the complex mechanisms Csy4 utilizes to recognize, position, and cleave its cognate RNA substrate in order to generate mature crRNAs. Csy4 makes sequence-specific contacts to the major groove of its cognate RNA stem-loop and makes extensive electrostatic interactions with the phosphate backbone that are highly sensitive to the helical geometry of the substrate, resulting in an extremely high affinity binding interaction (<italic>K</italic><sub>d</sub> &#8776; 50 pM). Csy4 has equally tight affinity for both its substrate and product RNAs and therefore functions <italic>in vivo</italic> as a single turnover catalyst. Phylogenetically conserved serine and histidine residues constitute a catalytic dyad in which the serine pins the ribosyl 2&#8242;-hydroxyl nucleophile in place, allowing the histidine to deprotonate the active site 2&#8242;-hydroxyl, leading to nucleophilic attack on the scissile phosphate. The Csy4 active site lacks a general acid to protonate the leaving group and positively charged residues to stabilize the transition state, explaining why the observed catalytic rate constant is ~10<super>4</super>-fold slower than that of RNase A. The RNA cleavage step carried out by Csy4 is essential for assembly of the Csy protein-crRNA complex that facilitates target recognition. Considering that Csy4 recognizes a single cellular substrate and subsequently sequesters the cleavage product, evolutionary pressure has likely selected for substrate specificity and high-affinity crRNA interactions at the expense of rapid cleavage kinetics.A major goal of synthetic biology is to construct reliable and predictable genetic circuits. However, synthetic genetic systems often perform unpredictably due to structural interactions between DNA, RNA, and protein components. Here we present a novel synthetic RNA processing platform utilizing Csy4 and its cognate target RNA to physically separate otherwise linked genetic elements such as promoters, ribosome binding sites, <italic>cis</italic> regulatory elements, and riboregulators. Implementation of this platform provides a general approach for creating context-free standard genetic elements that can be readily applied to the bottom-up construction of increasingly complex biological systems in a plug-and-play manner.
A Study of Corrugated Shelf-Ready Packaging (SRP) Appropriate to Colgate Palmolive Products (2007)

At the time of this study, each time a customer in Europe requested that Colgate Palmolive (CP) implement a Shelf Ready Packaging (SRP) solution for a product, CP did not have the data and tools necessary to weigh the impact of the request. Since most retail stores in Europe use a high level of SRP, it was important for Colgate Palmolive to master this type of packaging. The challenge of this thesis project was to address this situation by developing a set of practical guidelines for use by CP packaging engineers when developing SRP packaging solutions that met retailers' requirements and addressed CP's manufacturing constraints\nTo do this, it was first necessary to define the functional requirements of Shelf-Ready Packaging according to manufacturers and retailers in Europe. Next, existing SRP concepts used by Colgate Palmolive (CP) in Europe were mapped and benchmarked with the competition. The effectiveness of CP's use of SRP for selected SKUs in specific categories was then assessed using the Score Cards Assessment Tool (version 10) issued by ECR Europe in January 2007. The best calibration, providing the largest range of percentage of adherence to SRP, was recommended and applied in the rest of the study.\nTo build up a complete and useful database of SRP information, actual case studies involving five existing SRP concepts used by CP in Europe were conducted. Three major corrugate suppliers in Europe were each asked to rethink the concepts and to present their ideas for improvement and/or innovation. For each new proposal, an SRP card was created to easily identify its advantages and drawbacks and to document the technical recommendations. Finally, where possible, industrials trials for the more relevant proposals were conducted to determine the readiness of the proposed solutions for immediate implementation.\nThe knowledge gained from the research conducted and the case studies resulted in the creation of a practical manufacturing SRP bundle book, containing SRP Cards and Best Practice Guidelines, to be used by CP packaging engineers in future SRP development efforts.
Electronic portal imaging with an avalanche‐multiplication‐based video camera (2000)

The aim of this study is to investigate the degree to which the imaging quality of an existing (video-based) electronic portal imaging device (EPID) system may be improved by using an avalanche-multiplication-based video camera (called the avalanche-gain method). Due to avalanche multiplication in the target of the video camera tube, the new camera can be made up to several hundred times more sensitive than a camera using a conventional video (e.g., Saticon) tube. As a result, the camera noise which limits the performance of current video-based EPIDs should be overwhelmed and made negligible. The detective quantum efficiency (DQE) of an EPID using the avalanche-gain method has been measured with 6 MV and 18 MV beams obtained using a linear accelerator. It is shown that the camera noise is indeed much smaller than quantum noise and that the DQE of the system is significantly increased compared to conventional video-based EPIDs. Variation of DQE of the avalanche-gain video portal system with a change of demagnification was also investigated. It has been shown that the improvement of optical coupling has less effect in this system than that in a conventional video-based EPID system.
Enteric Viruses and Diarrhea in HIV-Infected Patients (1993)

Diarrhea occurs frequently among persons with the acquired immunodeficiency syndrome, but the cause often remains unknown. We used a group of diagnostic assays to determine which viruses were etiologic agents of diarrhea in a group of persons infected with the human immunodeficiency virus (HIV).
Zinc Iontophoresis to Treat Ischemic Skin Ulcers (1981)

Journal Article Zinc Iontophoresis to Treat Ischemic Skin Ulcers Get access Mark W Cornwall, BS Mark W Cornwall, BS 1Mr. Cornwall was Staff Therapist, Physician's Physical Therapy Service, Gary, IN 46409, when this study was done. He is now a master's degree student in Medical Allied Health Professions, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514 (USA). Search for other works by this author on: Oxford Academic Google Scholar Physical Therapy, Volume 61, Issue 3, 1 March 1981, Pages 359–360, https://doi.org/10.1093/ptj/61.3.359 Published: 01 March 1981 Article history Received: 06 February 1980 Accepted: 02 June 1980 Published: 01 March 1981
ChemInform Abstract: Enzymatic Peptidyl α‐Amidation Proceeds Through Formation of an α‐Hydroxyglycine Intermediate. (1989)

Abstract The capacity of a homogeneous α‐amidating enzyme (not further specified) to convert (I) or its N‐ and α‐hydroxyglycine analogues (III) and (IV) to (II) is examined.
Tissue PCO2 as universal marker of tissue hypoxia. (2000)

The author reviews the relationship between tissue PCO2 and tissue perfusion; the use of gastric tonometry and sublingual tonometry are described.
Optimized Regression for Efficient Function Evaluation (2012)

In many applications of regression, one is concerned with the efficiency of the estimated function in addition to the accuracy of the regression. For efficiency, it is common to represent the estimated function as a rectangular lattice of values-a lookup table (LUT)-that can be linearly interpolated for any needed value. Typically, a LUT is constructed from data with a two-step process that first fits a function to the data, then evaluates that fitted function at the nodes of the lattice. We present an approach, termed lattice regression, that directly optimizes the values of the lattice nodes to minimize the post-interpolation training error. Additionally, we propose a second-order difference regularizer to promote smoothness. We demonstrate the effectiveness of this approach on two image processing tasks that require both accurate regression and efficient function evaluations: inverse device characterization for color management and omnidirectional super-resolution for visual homing.
Feedforward regulation of mRNA stability by prolonged extracellular signal‐regulated kinase activity (2014)

Extracellular signal-regulated kinase (ERK) plays a central role in signal transduction networks and cell fate decisions. Sustained ERK activation induces cell differentiation, whereas transient ERK results in the proliferation of several types of cells. Sustained ERK activity stabilizes the proteins of early-response gene products. However, the effect of ERK activity duration on mRNA stability is unknown. We analyzed the quantitative relationship between the duration of four ERK activity kinetics and the mRNA expression profile in growth factor-treated cells. Time-course transcriptome analysis revealed that the cells with prolonged ERK activity generally showed sustained mRNA expression of late response genes but not early or mid genes. Selected late response genes decayed more rapidly in the presence of a specific ERK inhibitor than a general transcription inhibitor and the decay rate was not related to the number of AU-rich elements. Our results suggest that sustained ERK activity plays an important role in the lifespan of the mRNA encoded by late response genes, in addition to the previously demonstrated role in protein stabilization of early-response genes, including transcription factors regulating the transcription of mid and late genes. This double-positive regulation of ligand-induced genes, also termed feedforward regulation, is critical in cell fate decisions.