gebhart/patrepeval huggingface dataset:
Median charge and charge heterogeneity of human pituitary FSH, LH and TSH (1985)

The median charge and the degree of charge heterogeneity of FSH, LH and TSH in extracts from pituitaries of 63 individuals were determined by electrophoresis in 0.17% agarose suspension at pH 8.6 followed by hormone analyses by radioimmunoassay. Charge was expressed as migration rate in relation to that of human serum albumin. FSH, LH and TSH showed similar degrees of charge heterogeneity within the pituitary, and this degree of heterogeneity was not related to sex or age. There was a sex- and age-related variation in median charge of FSH and LH but not of TSH. The forms of FSH and LH were more acidic at higher ages and the most acidic forms were found in elderly women. Women 32 to 47 years old had more acidic forms of FSH than those 14 to 20 years of age. The median charge of LH was similar in young men and women, while that of FSH was more basic in young women than in young men. These results indicate that the effect of gonadal feedback on the molecular charge is different for FSH and LH. Studies with neuraminidase-treated pituitary extracts indicated that the sex- and age-dependent variations of the charge on FSH were due to differences in the sialic acid content. The amount of FSH in the pituitary was significantly smaller in men than in women. The contents of TSH and LH in the pituitary were lower in the higher age groups. No such decrease with age was found for FSH.
N-Substituted cyclopropylamines as monoamine oxidase inhibitors. Structure-activity relations. Dopa potentiation in mice and in vitro inhibition of kynuramine oxidation (1968)

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTN-Substituted cyclopropylamines as monoamine oxidase inhibitors. Structure-activity relations. Dopa potentiation in mice and in vitro inhibition of kynuramine oxidationJack Mills, Richard W. Kattau, Irwin H. Slater, and Ray W. FullerCite this: J. Med. Chem. 1968, 11, 1, 95–97Publication Date (Print):January 1, 1968Publication History Published online1 May 2002Published inissue 1 January 1968https://pubs.acs.org/doi/10.1021/jm00307a021https://doi.org/10.1021/jm00307a021research-articleACS PublicationsRequest reuse permissionsArticle Views112Altmetric-Citations18LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts
Structural characterization of a biologically active human lipocortin 1 expressed in <i>Escherichia coli</i> (1993)

Lipocortin or annexin 1 is a calcium-dependent phospholipid-binding protein which probably acts as a glucocorticoid- regulated anti-inflammatory factor. cDNA for human lipocortin 1 was cloned in the pT7.7 expression plasmid under the control of the inducible bacteriophage T7 RNA polymerase promoter. Upon induction with isopropyl thio-beta-D-galactoside, large amounts of the protein were produced and accumulated in Escherichia coli in a soluble form. The recombinant protein was purified to homogeneity by means of two subsequent ion-exchange chromatographic steps. The final yield was about 30 mg/l bacterial culture. Electrospray mass spectrometric analysis of the purified protein demonstrated that the recombinant product corresponds to the native human lipocortin 1, without the initial methionine and with a free N-terminal alanine; tryptic peptide mapping by fast-atom-bombardment mass spectrometry showed that the recombinant protein contains cysteine residues at positions 263 and 324 with free thiol groups, whereas Cys270 and Cys343 are probably involved in an intrachain disulfide bridge. Recombinant human lipocortin 1 reduces the carrageenin-induced paw oedema in rat in vivo and inhibits porcine pancreatic phospholipase A2 activity in vitro; in both cases, a dose-related response is observed.
Down-regulation of terminal deoxynucleotidyl transferase by Ig heavy chain in B lineage cells (1997)

The enzyme terminal deoxynucleotidyl transferase (TdT) adds nontemplate-derived nucleotides (N regions) to the junctions between recombining variable, diversity, and joining segments of Ig genes. The relative paucity of N regions in Ig light chains, together with the down-regulation of TdT transcription in pre-B cells (prior to light chain production), suggested that production of IgM heavy chain (mu) protein might negatively regulate TdT expression. In this study, we examined the effect of mu production on TdT gene expression in B lineage subsets from normal mice, from recombination-deficient mice (SCID and Rag-1-) carrying mu transgenes, and in transformed pro-B cell lines transfected with mu constructs. In normal mice, TdT is sharply down-regulated at the early pre-B stage in which cells have just completed productive mu rearrangement. Furthermore, the expression of mu transgenes in pro-B stage cells from recombination-deficient mice results in a similar decrease. Finally, transfection of genomic constructs encoding mu into pro-B cell lines results in a marked reduction of TdT expression. Taken together, these findings indicate that mu protein production results in the down-regulation of TdT. The ability of mu transgenes to alter TdT expression in cell lines also suggests that signaling through the pre-B receptor does not necessarily require interaction with an external stromal cell-derived ligand.
Modulation of stimulatory action of follicle stimulating hormone (FSH) and inhibitory action of epidermal growth factor (EGF) on aromatase activity in Sertoli cells by calcium (1987)

Aromatization of testosterone by cultured Sertoli cells isolated from immature rats was stimulated more than 7-fold by follicle stimulating hormone (FSH) or dcAMP. The effects of FSH and dcAMP could be partly inhibited by epidermal growth factor (EGF) in a dose-dependent manner (ID500.5 nM). The phorbol ester 4 beta-phorbol-12-myristate-13-acetate (PMA) could also inhibit aromatase activity in a fashion similar to EGF. When 3 mM EGTA was present in the culture medium, the inhibitory effect of EGF was abolished but the stimulatory effect of FSH or dcAMP was magnified. These results suggest that EGF exerts a negative control on aromatase via calcium and protein kinase C. The abolishment of the inhibitory effect of EGF and the enhancement of the stimulatory effect of FSH or dcAMP by a calcium deficiency may be an indication that growth factors produced by Sertoli cells negatively controls FSH-induced responses in an autocrine fashion.
Dynamics of the Vascular System (2004)

Historical Background and Book Content Vascular Biology, Structure and Function Physical Concepts and Basic Fluid Mechanics Hemodynamics of Large Arteries Vascular Branching The Venous System The Microcirculation The Integrated Vascular System and Diseased Conditions.
Patient data synchronization process in a continuity of care environment. (2005)

In a distributed patient record environment, we analyze the processes needed to ensure exchange and access to EHR data. We propose an adapted method and the tools for data synchronization. Our study takes into account the issue of user rights management for data access and decreasing the amount of data exchanged over the network. We describe a XML-based synchronization model that is portable and independent of specific medical data models. The implemented platform consists of several servers, of local network clients, of workstations running user's interfaces and of data exchange and synchronization tools.
Chemical Bonding and Physical Trapping of Sulfur in Mesoporous Magnéli Ti<sub>4</sub>O<sub>7</sub> Microspheres for High‐Performance Li–S Battery (2016)

Various host materials have been investigated to address the intrinsic drawbacks of lithium sulfur batteries, such as the low electronic conductivity of sulfur and inevitable decay in capacity during cycling. Besides the widely investigated carbonaceous materials, metal oxides have drawn much attention because they form strong chemical bonds with the soluble lithium polysulfides. Here, mesoporous Magnéli Ti 4 O 7 microspheres are prepared via an in situ carbothermal reduction that exhibit interconnected mesopores (20.4 nm), large pore volume (0.39 cm 3 g −1 ), and high surface area (197.2 m 2 g −1 ). When the sulfur cathode is embedded in a matrix of mesoporous Magnéli Ti 4 O 7 microspheres, it exhibits a superior reversible capacity of 1317.6 mA h g −1 at moderate current (C/10) and a low decay in capacity of 12% after 400 cycles at C/5. Strong chemical bonding of the lithium polysulfides to Ti 4 O 7 , as well as effective physical trapping in the mesopores and voids in the matrix are considered responsible for the improved electrochemical performance. A mechanism of the physical and chemical interactions between mesoporous Magnéli Ti 4 O 7 microspheres and sulfur is proposed based on systematic investigations.
NuGO contributions to GenePattern (2008)

NuGO, the European Nutrigenomics Organization, utilizes 31 powerful computers for, e.g., data storage and analysis. These so-called black boxes (NBXses) are located at the sites of different partners. NuGO decided to use GenePattern as the preferred genomic analysis tool on each NBX. To handle the custom made Affymetrix NuGO arrays, new NuGO modules are added to GenePattern. These NuGO modules execute the latest Bioconductor version ensuring up-to-date annotations and access to the latest scientific developments. The following GenePattern modules are provided by NuGO: NuGOArrayQualityAnalysis for comprehensive quality control, NuGOExpressionFileCreator for import and normalization of data, LimmaAnalysis for identification of differentially expressed genes, TopGoAnalysis for calculation of GO enrichment, and GetResultForGo for retrieval of information on genes associated with specific GO terms. All together, these NuGO modules allow comprehensive, up-to-date, and user friendly analysis of Affymetrix data. A special feature of the NuGO modules is that for analysis they allow the use of either the standard Affymetrix or the MBNI custom CDF-files, which remap probes based on current knowledge. In both cases a .chip-file is created to enable GSEA analysis. The NuGO GenePattern installations are distributed as binary Ubuntu (.deb) packages via the NuGO repository.
TOPS: an architecture for telephony over packet networks (1999)

Packet telephony is of increasing interest in both the telecommunications and Internet communities. The emergence of packet telephony will create new services, and presents an opportunity to rethink how conventional telephony services are implemented. In this paper, we present an architecture for telephony over packet networks (TOPS). TOPS allows users to move between terminals or to use mobile terminals while being reachable by the same name. TOPS users can have multiple terminals and control how calls are routed to them. TOPS allows for terminals with a range of capabilities such as support for video, whiteboard, and other media with a variety of coding formats. TOPS retains the necessary information on terminal capabilities to determine the appropriate type of communication to be established with the remote terminal. The architecture assumes that the underlying network supports the establishment of end-to-end connectivity between terminals, with an appropriate quality of service. The components of TOPS are a directory service, an application layer signaling protocol, and a logical channel abstraction for communication between end-systems. The directory service maps a user's name to a set of terminals where the user may be reached. A user can control the translation operation by specifying profiles that customize how his name is mapped to a set of terminals where he can be reached. Terminal capabilities are also stored in the directory service. The application layer signaling protocol establishes and maintains call state between communicating terminals. The logical channel abstraction provides a shared end-to-end context for a call's constituent media and control streams, while isolating the applications from the details of the network transport mechanisms. In addition to supporting simple point-to-point calls, the architecture supports both centralized and decentralized conferencing. We also introduce a simple encapsulation format for voice.
A novel micromachined magnetic-field sensor (1999)

A novel micromachined magnetic-field sensor (MFS) using the Lorentz force is presented. Lateral displacement of surface micromachined structures is generated by the interaction of a defined current through a conducting beam and an external magnetic field. The horizontal displacement of the beam is converted into a capacitance change by comb-electrodes which form a differential capacitor. Electronic circuitry is described that measures the magnetic field by detecting the displacement of the conducting beam. If operated in vacuum at mechanical resonance, a large displacement amplitude is achieved. This magnification of the physical effect by resonant operation has been realized by a new vacuum encapsulation process. Measurements show a substantial magnification of amplitude as characterized by Q-factors above 50. First measurements with prototypes yield magnetic sensitivities of 60000 /sup V///sub A/spl middot/T/ with a detection limit in the dual digit /spl mu/T-range. With improvements in the measurement setup, lower detection limits in the single digit /spl mu/T range (that is one order of magnitude smaller than the earth's magnetic field) are expected. Possible applications are magnetic-field measurement devices and navigation systems.
NGS catalog: A database of next generation sequencing studies in humans (2012)

Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research since its advent only a few years ago, and they are expected to advance at an unprecedented pace in the following years. To provide the research community with a comprehensive NGS resource, we have developed the database Next Generation Sequencing Catalog (NGS Catalog, http://bioinfo.mc.vanderbilt.edu/NGS/index.html), a continually updated database that collects, curates and manages available human NGS data obtained from published literature. NGS Catalog deposits publication information of NGS studies and their mutation characteristics (SNVs, small insertions/deletions, copy number variations, and structural variants), as well as mutated genes and gene fusions detected by NGS. Other functions include user data upload, NGS general analysis pipelines, and NGS software. NGS Catalog is particularly useful for investigators who are new to NGS but would like to take advantage of these powerful technologies for their own research. Finally, based on the data deposited in NGS Catalog, we summarized features and findings from whole exome sequencing, whole genome sequencing, and transcriptome sequencing studies for human diseases or traits.
<title>Timing high-speed microprocessor circuits using picosecond imaging circuit analysis</title> (2001)

IBM Research has developed a time resolved imaging technique, Picosecond Imaging Circuit Analysis (PICA), which uses single photon events to analyze signals in modern microprocessors on a picosecond time scale. This paper will describe the experimental setup as well as the data management software. A case study of a particularly hard debug problem on a state of the art microprocessor will demonstrate the application of the PICA method.
Combinations of genetic algorithms and neural networks: a survey of the state of the art (2003)

Various schemes for combining genetic algorithms and neural networks have been proposed and tested in recent years, but the literature is scattered among a variety of journals, proceedings and technical reports. Activity in this area is clearly increasing. The authors provide an overview of this body of literature drawing out common themes and providing, where possible, the emerging wisdom about what seems to work and what does not.< <ETX xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">></ETX>
Pigs as ‘Mixing Vessels’ for the Creation of New Pandemic Influenza A Viruses (1990)

Human influenza A viruses do not spread in the bird population and vice versa. The viral component involved in keeping influenza A viruses of these two independently evolving reservoirs apart is the nucleoprotein. However, reassortants with a mixed genome are created through antigenic shift. This kind of recombination between avian and human influenza A strains seems to occur during double infection, specifically of pigs which are tolerant of becoming infected with influenza A viruses from both reservoirs. To minimize the probability of such antigenic shift, it is recommended that pigs are kept in closed farms, especially in southeast Asia, the starting point of most influenza pandemics.
CDDO-Me reveals USP7 as a novel target in ovarian cancer cells (2016)

Deubiquitinating enzyme USP7 has been involved in the pathogenesis and progression of several cancers. Targeting USP7 is becoming an attractive strategy for cancer therapy. In this study, we identified synthetic triterpenoid C-28 methyl ester of 2-cyano-3, 12-dioxoolen-1, 9-dien-28-oic acid (CDDO-Me) as a novel inhibitor of USP7 but not of other cysteine proteases such as cathepsin B and cathepsin D. CDDO-Me inhibits USP7 activity via a mechanism that is independent of the presence of α, β-unsaturated ketones. Molecular docking studies showed that CDDO-Me fits well in the ubiquitin carboxyl terminus-binding pocket on USP7. Given that CDDO-Me is known to be effective against ovarian cancer cells, we speculated that CDDO-Me may target USP7 in ovarian cancer cells. We demonstrated that ovarian cancer cells have higher USP7 expression than their normal counterparts. Knockdown of USP7 inhibits the proliferation of ovarian cancer cells both in vitro and in vivo. Using the cellular thermal shift assay and the drug affinity responsive target stability assay, we further demonstrated that CDDO-Me directly binds to USP7 in cells, which leads to the decrease of its substrates such as MDM2, MDMX and UHRF1. CDDO-Me suppresses ovarian cancer tumor growth in an xenograft model. In conclusion, we demonstrate that USP7 is a novel target of ovarian cancer cells; targeting USP7 may contribute to the anti-cancer effect of CDDO-Me. The development of novel USP7 selective compounds based on the CDDO-Me-scaffold warrants further investigation.
A Monitoring Approach for Safe IPv6 Renumbering (2006)

Network renumbering is a very interesting feature of IPv6.It is also one of the most risky procedure which needs special attention in the management plane.In this paper we identify the challenges of renumbering and demonstrate how monitoring can improve this process.We also present an implementation of a monitoring framework and share the experience gained in its deployment.
Molecular aspects of Mg2+ transport systems. (1993)

The gram-negative bacterium Salmonella typhimurium possesses three distinct Mg2+ transport systems, encoded by the CorA, MgtA, and MgtB loci. The CorA transport system is the constitutive Mg2+ influx system but can also mediate efflux at high extracellular Mg2+ concentrations. The CorA protein lacks homology to any known protein, is an integral membrane protein containing 28% percent-charged amino acids, and has three carboxyl terminal membrane-spanning segments. Its properties indicate that it is a new class of membrane transport protein, likely found in all gram-negative bacteria and possibly other organisms. In contrast, the MgtA and MgtB Mg2+ transport systems are normally expressed only at low extracellular Mg2+ concentrations and can mediate only the influx of Mg2+. Both MgtA and MgtB system are P-type ATPases; they have relatively poor homology to other known prokaryotic P-type ATPases but are highly homologous to mammalian P-type ATPases, particularly reticular Ca(2+)-ATPases. Expression of both MgtA and MgtB is highly regulated by the concentration of extracellular Mg2+. Transcription of MgtB is increased about 1,000-fold by lowering Mg2+ from 1 mM to 1 microM, and, under growth conditions of limiting Mg2+, MgtB becomes the dominant Mg2+ influx system. However, it is unclear why the cells require the use of ATP to mediate the influx of Mg2+ down its electrochemical gradient. Study of these Mg2+ transport systems should lead to further understanding of cellular Mg2+ homeostasis and eventually to characterization of eukaryotic Mg2+ transport systems.
The Virtual Lens (1998)

We describe a new type of feedback display based upon ocular accommodation, called the virtual lens, that maintains a focused projection of a CRT image on the retina independent of changes in accommodation, and that replaces the optical image-processing action of the crystalline lens with an arbitrary computable image transform. We describe some applications of the virtual lens in visual psychophysics and virtual environments.
Magnetic resonance imaging in patients with ICDs and pacemakers. (2005)

Magnetic resonance (MR) imaging has unparalleled soft-tissue imaging capabilities. The presence of devices such as pacemakers and implantable cardioverter/defibrillators (ICDs), however, is historically considered a contraindication to MR imaging. These devices are now smaller, with less magnetic material and improved electromagnetic interference protection. This review summarizes the potential hazards of the device-MR environment interaction, and presents updated information regarding in-vivo and in-vitro experiments. Recent reports on patients with implantable pacemakers and ICDs who underwent MR scan shows that under certain conditions patients with these implanted systems may benefit from this imaging modality. The data presented suggests that certain modern pacemaker and ICD systems may indeed be MR safe. This may have major clinical implications on current imaging practice.
Effects of dietary fucoxanthin on cholesterol metabolism in diabetic/obese KK-A y mice (2012)

Fucoxanthin increased HDL-cholesterol and non-HDL-cholesterol levels in KK-A(y) mice by inducing SREBP expression and reduced cholesterol uptake in the liver via down-regulation of LDLR and SR-B1, resulted in increased serum cholesterol in the mice.